LOCUS       DASCNS010000001         9267 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000033580, whole genome shotgun sequence.
ACCESSION   DASCNS010000001 DASCNS010000000
VERSION     DASCNS010000001.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000033580"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="QF838_00005"
     CDS             <1..326
                     /locus_tag="QF838_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012982.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HJO59111.1"
                     /translation="SERRRRSLKSEYFRFAITDRGIPDITYMKDLLDLIDSQIESNIP
                     VYIHCFRGLGRTGTVVGCYLVRHGFTGEEALDEIMNLRSGVAGSFRASPETQIQRDFV
                     MDWQE"
     gene            complement(327..821)
                     /locus_tag="QF838_00010"
     CDS             complement(327..821)
                     /locus_tag="QF838_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015999.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HJO59112.1"
                     /translation="MTSAGNIESEIKQTLEILGITYSWIVVDPDFADTENFCRKYDYP
                     MEKSGNTILVASKRGEKKYCACLVLATAKLDVNKKVKELMGVSRLSFANTEETTDLTG
                     MMIGGVTPIGLPDTLSLFIDSDVMASDYVIIGGGSRSGKIQVSPQELLKLPNSKVVPG
                     LSKS"
     gene            complement(853..1881)
                     /locus_tag="QF838_00015"
     CDS             complement(853..1881)
                     /locus_tag="QF838_00015"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO59113.1"
                     /translation="MTRPHGLLRAGYPYSTTLGMRRVTNFPTDIAFGEENILYVLLRS
                     EGVATIRIWSRDDMEELTDELKGFGNFGCEDGQLRWPVNIITDDSGNIFISDEANHRI
                     SKFDPDGGFISKFGRHGQGEGEFDGPSGFALDINEHFIICDSKNSRIQRYTKDGEYIN
                     SFGSYGSEPGQFNLPWGVDCDESGDIYISDWGNHRVQIFSPDGDLKNVVGEFGSGNGQ
                     FNRPTGITVDNHGDFYVADWGNNRVLMYNSKGQYIWSFYGDATLSKVARNYMLTNAVP
                     NRLREMGKLESEKFLRRPRSVRIDDDFRLYIPDFESYRIQVYQKDFIELDQTQYAGPL
                     RNPTLEVT"
     gene            complement(1900..3036)
                     /locus_tag="QF838_00020"
     CDS             complement(1900..3036)
                     /locus_tag="QF838_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HJO59114.1"
                     /translation="MVTEAKEKTLVVIQLTGGNDFINTIVPYNNEHYYDARKKIVITQ
                     TDVLPINEELAINNNARPLKRLFDEGKVAIVQGIGYPNSNRSHFRGMDIWHTCEPDRV
                     GSEGWLGLAIRDLDPKSENVLTGVNIGQGLPRAMSVAGVPITSVGDLEGYGVMNKIEQ
                     ERLKERSLQAFKDIYGQAIGSGQVAEYIGKTGLDVLKGADLLANVATQYKSSVEYADN
                     SIAKSLRDVARVHFANLGTRVFYTNHGGYDTHANEMPTHPKLLSDLSGAISDFMDDLK
                     EHDAADDVAILVFTEFGRRMRDNGSGTDHGSGGGAFLIGNNVKGGLYSEYPSLNPNDW
                     EHGEDLKHTIDFRGIYGTVLEQWLGLDAKPIVKGEFEQIKPFYI"
     gene            complement(3059..4450)
                     /locus_tag="QF838_00025"
     CDS             complement(3059..4450)
                     /locus_tag="QF838_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO59115.1"
                     /translation="MKSDRQLVAHLMRRAGFGATPQDLDSLTSDKSYEEIIDDLVTPE
                     RFEPIDMSYVERYYTGEPVAVHVGKWLYRMANTKRPLEEKMSLFLHHIFPVAWGKSEH
                     GPSLYREIEMFRSTGLTNFKHILLQLSQDPAMLFWLDNHENHKDEINENYGRELLELF
                     SMGVGNYTENDIKNASRAFTGWTFRQPLSLYPYGHHHADFEFLPEDHDYDEKVFLGHV
                     GNFDGEDIIDIIVEQNAAAKFLSRHLYNFFVEDEVQVPSWETIDPKNPQAIEELSKAY
                     MESKGDMRHILKTLFNSDFFKNSLTLSKVKSPTELIVGVIKQTGEFNTPTPGIHEFAV
                     TTLNGSAIEGPLAIMGQRLMNPPTVEGWHTGSEWIDSGTLSERIGFVEKQFADPTKPG
                     VSEMVSRAGNLDDNPSELVDRCLDLLGAVNVRHETYQSLTDYARELKGLEDSAGVHNL
                     IQMTASTVDYQFA"
     gene            4595..5815
                     /locus_tag="QF838_00030"
     CDS             4595..5815
                     /locus_tag="QF838_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HJO59116.1"
                     /translation="MIDTNFDVIKILSAGAKALGAGAIEESRWEIPTDHPDPIDLAVG
                     MPVPEGIPIASLAKHFSKVLSSGNYIEMEYEYGRGKIDLRELLNDPSNGFTSLAQTPD
                     NFILFNGGSGCMESFCATFLNPGDIVLVEGPSFSGTVETILKYQGKIIEVAMNPEGMS
                     YQAIEEIVARCKRSGEIIKGIYTIDDFHNPTGVTMSENNRRTISRLALENGIMILEDS
                     TYSGLFFENAPPLDMYSMNSGEGVLRMGTFSKTIATGLRLGWLQGSTEAIDAIGKTRS
                     DMGNAPFIQTAVSRFIDSSEYSNHVQRMRGLYKSQCKALVTSLTQHCSKYLEFIEPKG
                     GFFLWPKCIGVDSAALTKAAAEEGVIFPDGSLFYQDSALHDNHIRLAFSGNSPEKLGE
                     VGLRLRKAFEKLLD"
     gene            5935..6693
                     /locus_tag="QF838_00035"
     CDS             5935..6693
                     /locus_tag="QF838_00035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59117.1"
                     /translation="MASNGKVALVTGAGTGIGKSSVLQLLGDGYSVVLAGRRIEPLEE
                     TISEAGVDDSRAIAIPTDVGNPDSVAALFEKAEATFGRLDVLFNNAGIGSPRMNMEDL
                     PVEDWQRVVDTNLTGSFVCAQHAIRIMKNQTPQGGRIINNGSISAYTPRPDAVAYTTT
                     KHAITGLTKQISLDGRKYNIACSQVDVGNAATPMTARMQGGVLQANGETIAEPTFNVD
                     HVGETVLYISNLPLDANIQFVTIMATQMPYVGRG"
     gene            complement(6725..8770)
                     /locus_tag="QF838_00040"
     CDS             complement(6725..8770)
                     /locus_tag="QF838_00040"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO59118.1"
                     /translation="MNKEPLLKVENLSVTFFTPRGTVTAVREASFEISRGEVLGIVGE
                     SGCGKSTTAFAVMGYLPTTAETGGSISFEGKDISSLSKEDLRELRGNRIAMVYQDPTT
                     SLNPSMKVGIQVQEVISEHLNLDQTAIDEKVVELFESVGLADADQMGDRYPHELSGGM
                     QQRVMIAMALACDPDLLIMDEPTTGLDVTTEATILDLVASLKERVNAGILYVTHNLGV
                     IARVADRVAVMYAGQTVEQSNVNDLFASPRHPYTSGLLSCVPKPPGTGEVSSRLKRIP
                     GFVYSSQEANVSSCLFAERCPIADSECTTSVPALEFIGQDSQVRCFHSDKVEPDTWGG
                     VEERISRKDVASPIVLTGNEITHQYGKRRKKYMAFGPDIDLPVRALNKIDFEIPQGKT
                     LGIVGESGSGKSTLARAVVGLIKAQDGLLQLHGNELDKTVENRSNEDKSAIQMVFQNP
                     TASLNPKLKIKKTLIRSLMRFSKLTKQGSEERAAALMEEVGLDPSYLERLPSELSGGE
                     QQRVAIAGAFATNPEIVVADEAVSALDVSVQAQVLSLLEDRQQELGNSYLFITHDLGV
                     VRYVSDDIMVVYAGHIAEYGPSEAVLSSPSHPYTEALLSAAPIPDPSAKPNPLRLEGA
                     VPTMREEFPGCFFASRCPRKLGSVCDTEAPPVQKASANENHVINCHIPVKDLEIIQA"
     gene            complement(8770..>9267)
                     /locus_tag="QF838_00045"
     CDS             complement(8770..>9267)
                     /locus_tag="QF838_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59119.1"
                     /translation="FPSLLLALLLITIGLQRTSLDESWLIIAVIGIAFTPNNSRVIRS
                     AALTIKPLEFVQNARLRGEPLYHIVLREILPNIVPVIGVEASLRLSFALLLTASLGFL
                     GLGVQPPTPDWGLMVNESRTHLALAPWASLAPAFAMGTLVIGVNLFADGIRQSLQLPE
                     DRGQK"
BASE COUNT         2470 a         2016 c         1934 g         2847 t
ORIGIN      
        1 attcagaacg gcgtcgccga tcactcaaat cagaatattt tcgatttgct ataactgacc
       61 gaggaatacc tgatatcact tatatgaaag acctattgga tctaatcgat tctcaaatag
      121 aatctaatat acctgtctat atacactgtt ttaggggttt aggtaggact ggcacagtcg
      181 ttggatgtta tctggttaga cacggtttca ctggtgagga agctttagat gaaataatga
      241 atttgaggtc aggagtcgca ggttcttttc gggcatcacc agaaactcaa atacaacgag
      301 atttcgtaat ggattggcaa gagtagttaa gatttagaca accctggaac aactttggag
      361 ttgggtaatt tgagtagttc ttgcgggctt acctgtattt tacctgatct acttcctccg
      421 cctattatga cgtaatcact tgccatcaca tcactgtcta tgaaaagtga tagtgtgtca
      481 ggtagaccaa taggagtcac accgcctatc atcataccag ttaggtcggt tgtctcttca
      541 gtattggcaa aagagagtct ggagactccc atcaattctt taactttttt atttacatca
      601 agtttggcgg tggctaaaac gagacaagcg caatattttt tctcacctct cttagaggct
      661 accaaaatgg tatttcctga tttttccata gggtagtcgt actttctaca gaaattctca
      721 gtgtccgcaa aatccggatc tacaacaatc cagctgtagg ttatgcctaa tatttccaag
      781 gtctgtttaa tttccgattc aatgtttcct gcacttgtca tttaggtatg tcctagttat
      841 aagagtgaag tattaagtta cttctaaagt agggtttcgc aaaggtcctg cgtactgagt
      901 ttggtctagt tctatgaagt ctttttgata aacttggatt ctgtaagatt caaaatcagg
      961 tatatacaat ctgaagtcat cgtcaattct cactgatcgg ggtcttctca aaaatttttc
     1021 agactcgagc tttcccattt cccttaaccg gtttggaaca gcgttggtca acatataatt
     1081 ccgggctacc ttcgataaag tggcgtcacc ataaaaactc catatgtact gaccctttga
     1141 attgtacata agaactctgt tattacccca gtctgccaca taaaaatccc cgtggttgtc
     1201 tacagtaatg ccggttggtc tattgaattg tccattacca cttccaaatt cacccacaac
     1261 gtttttgaga tcaccatctg gcgaaaaaat ttgtactctg tgattacccc aatcagaaat
     1321 atatatatct ccactctcat cacaatctac accccatgga agattaaatt gaccaggttc
     1381 cgagccatag gaaccaaagg aatttatata ttcaccatct tttgtgtatc tctgtatccg
     1441 actatttttc gaatcacata ttatgaaatg ttcgttaatg tccaatgcaa aacctgatgg
     1501 gccatcgaat tctccttcac cttgaccatg cctgccgaat ttgcttatga acccaccgtc
     1561 tggatcaaat ttgcttatcc tgtgattggc ttcatcactg atgaatatgt ttcctgaatc
     1621 gtccgtaatt atgttgacag gccatctcag ttgcccatct tcacatccga aattaccaaa
     1681 tccttttagc tcatctgtga gttcttccat atcgtctcga gaccagattc ggatggtggc
     1741 gacaccttct gatctaagca acacatataa aatattttcc tcgccaaatg cgatatctgt
     1801 aggaaaattg gttactcgtc tcattcctaa agttgtggaa tacggataac ctgctctcaa
     1861 taggccatgt ggtcgagtca tcgtaggtct cctaatgtgt tagatataga aaggttttat
     1921 ctgctcgaat tcgcctttta caattggctt cgcatcgagc cctaaccact gctctaatac
     1981 ggtgccatat attccacgga aatctatagt gtgctttaaa tcctctccat gctcccagtc
     2041 gttaggattt aaggagggat attcggaata aagtcctcct ttcacattat taccaattag
     2101 aaaagctccc ccacctgatc catgatctgt accactaccg ttgtctcgca ttcgtctacc
     2161 aaattctgtg aagaccagaa tggcaacatc gtcggccgca tcatgttctt taagatcatc
     2221 cataaagtca gaaatggctc cactcagatc actcaataac ttgggatgtg taggcatttc
     2281 attggcatgg gtatcgtatc caccatggtt ggtataaaag accctggttc ctaaattagc
     2341 aaaatgtacc cgagccacgt ctctcaaact tttagctata gagttgtcgg catattccac
     2401 agaggatttg tactgagtgg ccacatttgc cagtaagtcg gcccctttta agacatctaa
     2461 tccggttttc ccgatatatt cggctacctg cccacttccg atagcttgcc cgtaaatatc
     2521 tttgaaggct tgcaaggatc tttcctttaa gcgctcttgt tctattttgt tcatcacccc
     2581 atagccttcc aaatctccta ctgaagttat aggtactcct gcaacagaca tagccctggg
     2641 tagcccttgg cctatattaa ctccggtgag gacattttca cttttagggt ctaggtccct
     2701 gatggccaac cccaaccatc cttcactacc aactctgtca ggttcgcacg tatgccatat
     2761 atccattcca cgaaaatgag atctgtttga gtttggatat ccaattcctt gaactatagc
     2821 cacctttcct tcatcaaata gtcttttcaa aggcctggca ttattgttga tagctaattc
     2881 ttcgttaatg ggtagtacat cagtttgagt tattacaatt ttctttcgag catcgtaata
     2941 atgttcgttg ttataaggga ctatggtatt tataaaatcg ttaccgccag tcaattggat
     3001 tacgacaagc gttttttctt tagcttcagt gaccatatta gttccctctc cttatgctct
     3061 atgcaaattg gtaatctacg gtagatgctg tcatctggat gagattatga actccagcag
     3121 aatcctctaa tcctttcagc tctcgagcat aatctgttag ggattggtag gtttcatgcc
     3181 gtacatttac cgccccgagc aaatctaggc acctgtctac taattcagac ggattgtcgt
     3241 ctaaatttcc agctcggctc accatctcgg agactccggg tttcgtagga tctgcaaact
     3301 gtttttctac gaatcctatc ctctcactca acgtgcccga gtcgatccat tcggatccag
     3361 tatgccagcc ttccacagtg ggcggattca taagtctttg gcccatgata gcaagcggcc
     3421 cctcaattgc agatccgtta agggtggtaa ctgcaaactc gtgaattccc ggggtcggag
     3481 tgttgaattc acctgtttgt ttaataaccc caactataag ttctgtggga cttttcactt
     3541 tggacaaggt caaagaattt ttaaaaaagt cagaattaaa caaggtcttt aatatgtgtc
     3601 tcatatcccc tttggattcc atgtatgctt tggatagttc ttcgatggcc tgagggtttt
     3661 tagggtctat tgtttcccat gaaggtacct gaacttcatc ttccacaaaa aaattgtata
     3721 gatgccgtga caggaattta gctgcggcat tttgttcgac aatgatatct attatgtcct
     3781 cgccatcaaa attaccaaca tggcccagga atactttttc atcatagtca tgatcttcag
     3841 gaaggaattc gaaatcagca tgatgatgtc catatgggta aagcgataat ggttgcctga
     3901 aagtccatcc tgtaaatgct ctagaagcat tttttatgtc attttcggtg taattaccaa
     3961 cgcccatgga gaacaactct aaaagttctc ttccataatt ttcatttatt tcatctttgt
     4021 ggttttcatg attgtcgagc caaaaaagca tagctggatc ttgagacagt tggagtagga
     4081 tatgtttaaa atttgtcaat ccagtactac gaaacatttc tatttctctg tataaagatg
     4141 gaccatgctc actcttaccc catgcgacag ggaatatgtg atgtaaaaac agggacattt
     4201 tttcttccaa cggtctctta gtgttggcca ttctgtaaag ccatttcccg acatgaacag
     4261 ctacaggttc acctgtatag tatcgctcaa cataagacat atcgattggt tcaaaccttt
     4321 ctggggtcac caaatcatct atgatttctt cataagattt gtcagatgtc aggctatcta
     4381 aatcttgggg ggtggccccg aaacctgctc tcctcatcaa gtgtgctaca agttgtctgt
     4441 ctgatttcat agcatttccc ttataaattt tcaccattat aacgaaagtg aagatacttt
     4501 ctaattctag aatgtgaggc gtacgtttcc ggtctgtttg attatcaacc cgcgcggtac
     4561 aattatttag ttatttgtag aaatgaggta atccatgata gatactaact ttgatgttat
     4621 aaagattctt tcggcgggag ctaaagcttt aggcgccggc gccatagaag aaagtaggtg
     4681 ggaaatccca actgatcatc ctgatcctat tgatcttgca gttggaatgc ctgtacctga
     4741 agggatacct atagcatctt tggcgaagca tttctctaaa gttttatctt cgggtaacta
     4801 tatagagatg gagtatgagt atggtagagg gaagatcgat ttaagagaac tgttaaatga
     4861 tccaagtaac ggattcacct ctctggctca aactccagac aatttcatac ttttcaacgg
     4921 tggatctggg tgcatggaaa gtttttgcgc aactttttta aatcccggag atatcgtttt
     4981 ggttgaaggc ccaagtttct ctggcacagt tgagactatc cttaagtatc aaggtaaaat
     5041 catagaagtg gcaatgaacc cggaagggat gtcctatcag gctattgagg aaatagtagc
     5101 gaggtgcaag cgaagtgggg agataataaa gggaatatac accatagacg attttcacaa
     5161 ccctacgggt gtaacgatgt ctgaaaataa tcgtcgtacg atcagccgat tagccttaga
     5221 aaatggaatt atgattttag aggactctac atatagtggg ttgttttttg aaaatgcacc
     5281 tccccttgat atgtattcta tgaacagtgg ggaaggggta ttacgtatgg gcactttcag
     5341 caaaactatt gctaccgggc ttagattagg ttggctacaa ggttcaactg aggctatcga
     5401 tgccattgga aaaaccaggt ctgatatggg aaatgctcct ttcatacaga cggcagtaag
     5461 taggtttata gactcatctg aatattcaaa ccacgtgcaa aggatgagag ggctgtacaa
     5521 gtcccagtgc aaagcacttg ttacctctct gacccagcat tgctcaaagt atttggaatt
     5581 tattgagcct aaagggggtt ttttcctctg gccaaagtgt ataggggttg attcagcggc
     5641 attaacaaaa gctgccgctg aagaaggggt aatattccct gatgggtctt tgttttacca
     5701 agattcggct ctccacgata atcacataag gcttgctttt agtggtaact ctccagaaaa
     5761 gctgggcgaa gtagggttac gtttgaggaa agcttttgaa aaattattgg attaatatat
     5821 ttctttgctt ataaatacat atacctgatg accgatgatt tattgtttag gtgaccttcc
     5881 cattaatatc cgttaaaatc cgttccctaa tcacttaata aggagttcta tatcatggca
     5941 tctaatggaa aggtggcgct agtaacaggc gcaggtacag gcatcggtaa aagttcagtg
     6001 ttgcagctgt tgggggatgg ctactccgtt gtgttggctg gccgccgaat agaaccttta
     6061 gaagaaacta tttctgaagc tggcgtggac gattcaaggg ctatcgctat tcctactgat
     6121 gttggtaacc cagactccgt cgctgctctt tttgaaaagg ctgaagctac cttcggcaga
     6181 ttggatgttc ttttcaataa tgctggtatt ggatctccaa gaatgaatat ggaagatctt
     6241 ccagtagaag actggcaacg ggttgtcgat actaatctca ctggtagttt tgtttgtgca
     6301 cagcacgcca taagaataat gaaaaatcaa accccccagg gtggaagaat cataaataat
     6361 ggttcgattt ctgcttatac ccctaggcct gatgccgttg cttacactac gaccaagcat
     6421 gctattacag gccttacaaa gcagatatct ttagacggta ggaaatacaa cattgcgtgt
     6481 agtcaagttg atgttggcaa tgcggctacc cctatgacag ctaggatgca aggcggggta
     6541 ttgcaagcaa acggtgaaac gatcgctgaa ccgactttta atgtggatca tgtgggtgaa
     6601 actgtattgt acatctcaaa cttgcctcta gatgcaaata ttcagttcgt tacgataatg
     6661 gcgacccaga tgccttacgt cggaagaggt tagaaaacta acgtatcaac agtctttttg
     6721 tgttttaggc ttgaattatt tctaaatctt tcacagggat gtgacagttt attacatggt
     6781 tttcattagc agatgctttc tgtactggtg gcgcttctgt gtcacagaca gatcctaatt
     6841 tgcgagggca tcgactagca aagaaacagc cagggaactc ctcacgcata gttgggacgg
     6901 caccttctaa tctgagtgga ttaggtttcg cagaaggatc tggaatggga gctgccgata
     6961 aaagggcttc agtatagggg tgagatggac tgcttaggac agcctctgaa ggtccgtatt
     7021 ctgctatgtg acccgcatag accaccatta tgtcatcgga gacgtatctc acgaccccaa
     7081 ggtcatgagt tatgaagaga tatgaattgc ctaattcctg ctgccgatct tcaagtaggc
     7141 ttaagacttg agcttgtact gaaacgtcca atgctgacac agcttcatca gctactacta
     7201 tttcagggtt cgtagcaaaa gccccagcta tagcaactct ttgttgttct cctccactaa
     7261 gttcggatgg gagtctttcc aaataacttg ggtctaaccc tacttcttcc attaaagctg
     7321 cagctctttc ttcacttccc tgtttagtca actttgaaaa acgcataagt gatcggatta
     7381 gagtcttttt tattttgagt ttagggttta gggaggcagt cgggttttgg aaaaccattt
     7441 gtattgctga tttgtcttcg ttcgagcggt tttccaccgt tttatctaat tcattgccat
     7501 gcaactggag caatccatct tgggccttta tcaggcccac tacggccctg gcgagggtag
     7561 attttccaga accgctttcc cctacgatcc ccaaggtttt accttgcggt atttcaaaat
     7621 ctatcttgtt gagggctctg actggtaaat ctatgtcagg tccaaaagcc atatatttct
     7681 ttcgtcgctt tccatattgg tgtgttattt catttccagt taacacaatt gggctggcta
     7741 catctttgcg gcttattctt tcttctactc ctccccatgt atcaggttcg actttgtcgc
     7801 tatgaaaaca tctcacttga gagtcttgac cgataaattc cagagccgga acagatgtcg
     7861 tgcattctga atctgcaatt gggcaacgtt ccgcaaacaa acaagaacta acgttggctt
     7921 cctgcgatga ataaacaaat ccggggattc tttttaatct tgaggaaact tctccagttc
     7981 ctggaggttt aggaacacac gaaagaagac cagaggtata tgggtgccta ggagaggcaa
     8041 ataaatcatt tacattggat tgctcaaccg tttggccggc atacataact gcgactcggt
     8101 ctgctactct ggcgattact ccaaggttgt gggtaacata aagtatccct gcgtttacac
     8161 gctcttttag gcttgccacg aggtctagta tggttgcttc agtagtcacg tctagcccgg
     8221 ttgtgggctc gtccattatt aacaggtcag ggtcacaggc tagagccatg gcgatcatca
     8281 ctcgttgttg catccctcca cttaattcgt gtggatatct gtctcccatt tggtctgcat
     8341 ccgctaaccc cacactttca aataactcca ccactttttc gtctatagct gtctggtcta
     8401 ggtttaaatg ttccgagatt acttcctgga cctgaattcc gactttcata gaaggattta
     8461 acgatgtagt agggtcctga tataccatgg cgattcggtt gcctctcaac tccctcaaat
     8521 cttctttgga tagggatgat atgtcctttc cctcaaatga tattgagcca cctgtctccg
     8581 ccgtcgttgg taaatatccc attacggcaa atgccgtagt tgatttaccg catcctgact
     8641 ctcctacaat tcctaatacc tcgcctcttg aaatctcaaa agaagcctct cttacggcag
     8701 taaccgtacc tctaggggtg aagaatgtca ctgataaatt ttctacttta agcaatggtt
     8761 ctttattcat tacttctgac ctcgatcctc agggagctga agggattgtc tgataccgtc
     8821 ggcgaataaa ttaaccccta tgacaagcgt gcccatcgcg aaggcgggag ccaaagaggc
     8881 ccaaggtgct agagctaaat gagttcggct ttcattgacc ataagccccc aatctggtgt
     8941 tggtggctgc actccgagtc ctagaaatcc tagagatgcg gtcaacaata aagcgaaact
     9001 gagcctcagg ctagcctcga ctccaattac agggactatg tttggcaaaa tttccctaag
     9061 cacgatgtga tataacggtt cgcctcggag tcgtgcattt tgaacgaatt ctagtggttt
     9121 tattgtgaga gcagcacttc taataacacg gctgttatta ggggtaaatg ctatccctat
     9181 cacagctatt atcagccacg actcgtctag tgacgttctt tgcaggccga tcgttattaa
     9241 caataaagct aacagcaaag atgggaa
//
LOCUS       DASCNS010000002        13118 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000039258, whole genome shotgun sequence.
ACCESSION   DASCNS010000002 DASCNS010000000
VERSION     DASCNS010000002.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13118)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000039258"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="QF838_00050"
     CDS             <1..839
                     /locus_tag="QF838_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJO59120.1"
                     /translation="LVNIIVQPKENKMKNYKNLSFSVNDHVGFLTLDRPEVMNALNRE
                     LTLEFHEILDELPELFPKIRVLVITGNGNAFCSGADLSRMGASGSENRRNKDKSTPGR
                     RRIQELASAIRNLPQPVIAAVNGAAVGAGLSIALASDIRIATEKSRFSAIFVKRGLVP
                     DTAASATIKAIAGHGIAAEMSLTGKIYDAEWALSKGLVNDVVAASDLMDECMKLAIEI
                     AGNPPLAVRQTKQLLRVRMHDWSDIVSDEDSAGEMLYDTEDQKEAVKAFLEKRNPNYI
                     GK"
     gene            839..1933
                     /locus_tag="QF838_00055"
                     /pseudo
     CDS             839..1933
                     /locus_tag="QF838_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            1937..2275
                     /locus_tag="QF838_00060"
     CDS             1937..2275
                     /locus_tag="QF838_00060"
                     /inference="COORDINATES: protein motif:HMM:NF027666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HJO59121.1"
                     /translation="MINIDIPQSVLGKSMTTVIKENEMGPRDYLYHAYKDIQRAVTYQ
                     GWKLIEYNVKGVRSTQLFNLTTDPNESSDLFGHPNYSENIDVMRNKMFQARRDFGDYL
                     DPFAKFWEGF"
     gene            complement(2286..2717)
                     /locus_tag="QF838_00065"
     CDS             complement(2286..2717)
                     /locus_tag="QF838_00065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HJO59122.1"
                     /translation="MKTLSKTNADILGEIVDLLIPAKENLPSPTKINVVEHIDDALSN
                     TSATRRIFVESLGDINNKQFLSLSKMEQIDLLEKYELSNGTFFSQLLRHTYNAYYTNP
                     IVVAGIGMTGKPPQPMGYTLEKGNLTLLDKVIKKGQIWRNP"
     gene            complement(2742..3155)
                     /locus_tag="QF838_00070"
     CDS             complement(2742..3155)
                     /locus_tag="QF838_00070"
                     /inference="COORDINATES: protein motif:HMM:NF017050.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HJO59123.1"
                     /translation="MPEIHDKVTIDPKLTDSFGIPSPKIEYILSENSRRMLDHAIGTA
                     TQVMKAAGAVEITHDPLKREAGWHLMGTAKMGTNKDESVVNKYGQTHDVDNLFIIDGS
                     IFVTAGAVNPTSTIQALALYIADHISSKSRDFLSQ"
     gene            complement(3167..4126)
                     /locus_tag="QF838_00075"
     CDS             complement(3167..4126)
                     /locus_tag="QF838_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012935.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HJO59124.1"
                     /translation="MESPIAPLMYNAVRGSTIHWSAHFPRLKPSDFRVKTLDNIADDW
                     PLSYFDLEPYFNLNDKMMGVAGLDGDPAYPPKSPRQTPPVGLGKLGDKIAAGFDELGW
                     HWCPSDSAINTVEYDGRPGCNNRGPCDLGCSIKAKSSTDVTYWPKALELGAELVTNAR
                     VREITVDRSGKATSAIYYDCNGEVREQYASIIVLASNGVGTPRILLNSTSSRFPNGLA
                     NLSGLAGKNLMFLPYSIVTGLFDEDLEGHKGPTPLNIICSEFYETDPGRNFVRGYAYQ
                     VVRSLAPLATALSGHIPWGENHHDAFKNRFNRTITFAVIGEVP"
     gene            complement(4220..4366)
                     /locus_tag="QF838_00080"
     CDS             complement(4220..4366)
                     /locus_tag="QF838_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013086.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HJO59125.1"
                     /translation="MSINNKKVDVLVIGAGASGAAFTWRLAKSGMKVMCLEQGDWIDP
                     STDY"
     gene            complement(4412..4705)
                     /locus_tag="QF838_00085"
     CDS             complement(4412..4705)
                     /locus_tag="QF838_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59126.1"
                     /translation="MLIVQRTYTPKPGAGGLIGLLKTVSQKTIEVGLPEVKVYRKFLG
                     SHGIIVTTQEWESIEVYNNSRTMVRQTTEITSIFEQIYPLLDSTHVTEILESV"
     gene            4837..5661
                     /locus_tag="QF838_00090"
     CDS             4837..5661
                     /locus_tag="QF838_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HJO59127.1"
                     /translation="MSALTEKEVLEIFSAGGSLAGKDLTGLNLHRSVLSGVDLSGADI
                     RGINFRGANLSGSNLSNVKSSEGILAEANLRDSDLSGSDLSDTYLMEARLYASDLSHC
                     DLTGSNLSGAFMTGGVNLCNSSLVSANLRGADLSGAIIRQADLTMATLRSVKLIGADA
                     SFSDFTSAALNGADMTKTNLSGAKLVNAVLNSSKLEFARLDNANLTQASLRRCILRGA
                     SLQMAKLAKTNLIFTDFQEADLTLADLSESNLRGAILLGANLKWTDLSLTEFDNSN"
     gene            complement(5749..6276)
                     /locus_tag="QF838_00095"
     CDS             complement(5749..6276)
                     /locus_tag="QF838_00095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJO59128.1"
                     /translation="MSDKTYSQPPEFNLDPEKNYKAIIEMDNGNKITIDLLSKEAPKT
                     VNNFVSLAKDGYYDGIMFHRVIPGFMAQSGDPTGTGSGGPGYNFDDEFHDDAKHDSPG
                     ILSMANRGVINGMGTNGSQFFITFAPTPHLDGLHSVFGRVSDGMETVLSISERDPGSA
                     TTAGDAMKSVTIIEE"
     gene            complement(6287..6730)
                     /locus_tag="QF838_00100"
     CDS             complement(6287..6730)
                     /locus_tag="QF838_00100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HJO59129.1"
                     /translation="MSKLDFNFKHTLRVRWKECDIQGIAYYGSYIDFIDVAQAEYFRN
                     LGILTHQADTRKNFDLAAVKVTLEYKSPAKIDELIDIFLRVDKIGRTSIDKRSEIYRS
                     DTDELLCSGQSISVNFDSELGKSRIVSTEIKDIIEQFESTGQINK"
     gene            6985..9846
                     /locus_tag="QF838_00105"
     CDS             6985..9846
                     /locus_tag="QF838_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59130.1"
                     /translation="MREITGGLYNMFKKALMKMSVIGIIGAAFVACGVVDVEQAQGVV
                     DLRARILEIQTKQVDPLVEQINAIEEQVAPIEAEIEALERQKEELYREAEGIGQQFDD
                     EMRERFDTTYMEGDQARQEFENEIQERHRELEEKERSLHEEQQRLHDELEEANQEKRQ
                     AIEDAGQDTWEALEFEMRAKEEEAQKVSKSMWRAWDDERDEAQQQVENLRREFEENNP
                     FNMEMEAIHQAWKDIDDLYFEIDVEQMQIEEARMELHDMMRPLEEQAHQLRREERDIQ
                     EQDPNQFGSSANSGQDANQMIREQHDKLEDLRRQLREATDEQQQAQTAPRQLNWDDHG
                     RKTEEIWADYEARTEEIALKRAQASDTTGAAGDASMAQAALDEININFQDQLAFQEAK
                     LLQAMESLQAFETGAAANDGSALRSQLIANIEGMRSEVNEHQNVMDSLPANLQDELGN
                     PEYHNISGQLADAESALANTPETNTVDTTNADGTTTAQTVENPVYAGALASVENLKAQ
                     LANIPEFILGEPYANPSHLERTISIGSLNQEISILEAELNTLSDQPTTADPAYTAALA
                     EKNSLDSLIHETREQWDAEVAAKQNIIDAGSGGAPDQGQLDAEIQDLMANAEADRDNA
                     LAALDAEFTVTAGTNADDSGGRAEEIRDEIENIENYISDLHNSKDQGHQAQNQALENV
                     RSEVREIEEQMEGMHNFMRELEDQQRPLHEKRMALDKERMVLDQKQREIEDQRGPWEE
                     ERNRYEEDLWSGFDDWQRTRQREIEDQQEAMWELVRVDMDQNRRDTEVGMKQMWKDFE
                     NESRDSHKGADREFKEKRKAIDQERQELEESRDNLGQAFEAEREEAIQEIENMRDELY
                     EERMAPIEEEIAYLDDEIGAKFASIEGLYQQQEDLAGQLEDLEAQVRELDQEAEFGLL
                     SVISGAIDNAEKLEQNPASLAGAAGVSLTDFLPSPPSRE"
     gene            complement(10148..11221)
                     /locus_tag="QF838_00110"
     CDS             complement(10148..11221)
                     /locus_tag="QF838_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HJO59131.1"
                     /translation="MSNLFTPITIRGEKIRNRLFVAPMCQYSANNGDGVATDWHMVHL
                     GTRAVGGAGLVMAEATAVSPEGRISPQDLGIWSDEQATVLQPIASFIDSQGATPSIQL
                     AHAGRKASHKRPWEGGDVLTKKDGGWDIVGPSSLEFDEGWIVPNELTIEDIDSIISDF
                     SVSAVRSVGAGFRVLELHLAHGYLACEFMSPLSNNRKDSYGGTLRNRCRLPLDIVSAV
                     RKVIPESMPLLVRISATEYMSDGWSLEDSIELSGWLQEEGVDMIDCSSGGNSPSQNLD
                     VFPGYQVPFAAAIRREVGILTGAVGLITDASQAEQTLRNGEADVILLGRELLRNPYWP
                     LNAQVELDGQDSVWPPQYVRARR"
     gene            complement(11246..11605)
                     /locus_tag="QF838_00115"
     CDS             complement(11246..11605)
                     /locus_tag="QF838_00115"
                     /inference="COORDINATES: protein motif:HMM:NF021612.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HJO59132.1"
                     /translation="MDFLLSWWLLLILSGCLFFVTSMCFKYKSAASELKSQIRSQSTR
                     YGQITEQFLPLVEAYPWDSKQFRFLGSPIDGIQFEEDKIVLVEFKSSSSQMSAKQRKI
                     KGLVEEGKVEFRLIRAG"
     gene            complement(11640..12794)
                     /locus_tag="QF838_00120"
     CDS             complement(11640..12794)
                     /locus_tag="QF838_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013755.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HJO59133.1"
                     /translation="MSLRAIDEIPKILTSSDDLEKVIEDSLSNRYVSIDIEGNGFFRY
                     PEFVCLIQLCVGEDVYLVDPLAIDDISALGKVLANDKIIKILHAGDYDIRSLNRDYNF
                     SFNNVFDTSLAAALLGSKKLGLDSILKEYIDADVTKDKKLQRSDWSLRPLSKEARKYA
                     ADDVRYLFRAMEVMTNKLENLGRISWLKEECERLQLVKFSPRDEDIAFLDVKGSKILD
                     GKALSVLQSLYSVREDEAIGRDRPPFKIVGDSVLVAIARNPYSNYSEIKGIGMWGRAE
                     VSKRIRKIAIEALSQPPIGRPRRQNTGTNVMSNKEREKANTRLKELKGWRKTKGESIG
                     IDPSLLWPTISLDRLSKDPSRFNEEISEPQVRKWQYLEFGKELEGKLACL"
     gene            12904..>13118
                     /locus_tag="QF838_00125"
     CDS             12904..>13118
                     /locus_tag="QF838_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HJO59134.1"
                     /translation="MVRIPPWSREETILALYAYMNHGRLRENSFEAKELSTYLRHLTD
                     PHTTSDPDKYRNPDAIALKLRKFAALDP"
BASE COUNT         3817 a         2687 c         2866 g         3748 t
ORIGIN      
        1 tcctggtcaa cattattgtg cagccaaagg aaaataaaat gaagaattat aaaaatttat
       61 catttagcgt aaatgatcat gtcggatttt tgacactcga caggcctgaa gtcatgaatg
      121 ctttaaatcg cgaactgacc ttggaattcc atgagatttt agatgaattg ccagagctat
      181 ttcctaaaat tcgtgtgcta gttattacgg ggaacggaaa tgcattttgc tctggcgcgg
      241 atttatctag aatgggtgcc tctggatcgg aaaatagaag gaataaagat aaatctactc
      301 caggacgtcg aaggatacag gaattggcct cagctattcg taatttaccg caaccagtga
      361 ttgctgctgt aaatggggca gcagtaggag ccggattatc tattgcgctt gccagtgata
      421 ttaggattgc tactgagaaa tcaagatttt cagccatttt tgtgaagcgt ggactagtcc
      481 cggatactgc ggcctcagct actatcaaag ctatagctgg ccatggtatt gctgcagaaa
      541 tgtcattgac cgggaagata tatgatgctg aatgggcatt gtcaaaaggc ctggtgaatg
      601 atgttgtcgc tgcttcggat ttaatggatg aatgtatgaa attagcgatt gagattgcag
      661 gaaatccacc tttggcggtt agacaaacta agcaattact tcgggttagg atgcatgatt
      721 ggagcgatat agtgtctgac gaggattcgg cgggggaaat gctttatgac actgaggatc
      781 aaaaggaagc cgtaaaagct ttcttggaga aaagaaaccc aaattacatt ggtaaataat
      841 gtcaaaacca aacattttag taatttttac tgacgaccaa cggtttgata ctgttcacgc
      901 tcttgggaat aagaatattt ccacgcctaa tattgactgg cttgtacaaa atggtacctc
      961 gtttaacaat gcttacatta tgggagggac tgacacggcg gtatgaatgc cgagtagggc
     1021 gatgttgatg acagggcgaa cattatttca ccttgaaaag tctggggagc atataccaga
     1081 ggatcacatt atgctgggag agactttagg tagagctgga taccattgtt gggggactgg
     1141 taaatggcac aatgggacag aatcctatac acgtagtttt caagatggag ccgacatcta
     1201 ttttggaggt ggatacgatc attggatggt acccgttcat tcttacgatc cgtcgggaac
     1261 ctatcctggg cgtatatcca aagatatgat cccggtgcac caatatgaac ctattgatac
     1321 ctatacggag agggtccaaa cttatcgggt tcctgctttg gtggaggaga agattgctga
     1381 tcatgttcat gccggagaac attccagtaa cttatttgct agccaggctg cagacttctt
     1441 tttaaattac gagaaagaag accctttctt ttgctacatt gcttttatgg caccacacga
     1501 tccccgaacc acccctcagg agttttacga tatgtatcct cccgagaaaa taggaatcga
     1561 ggaaaatttc atgagccagc atccttttga caatggggaa ttggatgctc gggatgaact
     1621 tttggtagca attcctagag aggaatttga agtaaaatgc cacatcgctg aatactatgc
     1681 catggtgtct catcttgact cacaggtagg agttattctg gacgcattaa aaacatcaga
     1741 tcagattgat aaaaccatca tagttttcgc gggagacaat ggaattgctt taggaagaca
     1801 tggccttttt gggaaacaaa atttatatga tcatagtgtt catataccaa tgattttttg
     1861 tgggccggga atacccagta atgctgttcg agagtcattg gtttacacaa cggatatata
     1921 cccaactata tgtgacatga tcaatattga tattcctcaa agcgttctgg gcaaaagcat
     1981 gactaccgtt ataaaagaaa atgaaatggg tcctcgcgat tacttatacc atgcatataa
     2041 ggacatccaa agagccgtga catatcaggg ttggaagttg attgaataca acgtaaaagg
     2101 agttcgatca acccaattat tcaacctgac cactgatccc aatgagtcga gcgatttatt
     2161 cggccatcca aattacagtg aaaatattga tgttatgaga aataaaatgt ttcaagctcg
     2221 tcgggacttc ggagactatt tggacccttt tgccaaattt tgggaaggat tttaaccaaa
     2281 tattactaag ggtttctcca aatttgccct ttctttatca ctttatctaa taaggtgagg
     2341 ttgccttttt ctaaagtgta acccatgggt tgaggtggtt ttcctgtcat gccgattccg
     2401 gcgacaacta tcgggtttgt gtaataggcg ttgtaggtat gacgcaggag ttgcgaaaaa
     2461 aaagtcccgt ttgataattc atatttttcc aacaagtcaa tttgttccat ttttgataat
     2521 gataaaaatt gtttattgtt gatatcacca aggctctcga cgaaaattcg tctcgttgca
     2581 gaagtatttg aaagggcatc gtctatgtgt tccacaacat taattttagt aggagagggt
     2641 agattttcct ttgctggtat aagtaaatct actatttcac ctaaaatatc agcattggtt
     2701 ttggatagtg ttttcataaa gtgggttcca ttaaatcctt gctactggct taggaagtct
     2761 ctagatttgg atgagatatg atctgcaatg tatagtgcta gcgcctggat agtggaagtt
     2821 gggttaactg ccccggcggt aacgaatata cttccatcaa tgataaataa gttatcgaca
     2881 tcgtgggttt gaccgtactt gtttactaca gattcatcct tgtttgttcc catttttgct
     2941 gttcccatca ggtgccatcc agcctctcgt tttagagggt catgggtgat ttctaccgca
     3001 cctgccgcct tcatgacttg agtagctgtt ccaatggcgt gatctaacat tcttcgacta
     3061 ttctcactca gaatatactc gattttaggg gagggtatac caaaggaatc tgtaagtttc
     3121 gggtctatcg tgactttatc atgaatttct ggcaaatctt cacctattac ggcacttcac
     3181 ctattacggc aaatgttata gttcgattga atcgattctt gaaagcatca tggtgatttt
     3241 caccccaagg gatgtgacca ctaagagctg ttgccaaagg agctaacgaa cgaacgactt
     3301 gataagcata ccctctcaca aaatttctac caggatctgt ttcgtagaat tctgaacaaa
     3361 ttatattcaa aggggttggt cccttgtgtc cctctaaatc ctcatcaaat agacctgtga
     3421 ctattgaata aggaaggaac atcaagttct tccccgccag gccacttaaa ttggctaggc
     3481 cattagggaa tctggatgat gttgaattta atagaatcct aggcgtacca acaccatttg
     3541 aggcgagtac aatgattgaa gcatattgtt ctcttacttc accattacaa tcataatata
     3601 tggcacttgt tgcctttcca gatctatcaa cagtaatctc tctaacacgg gcgttagtaa
     3661 ccaattcggc gccgagctcc aaagctttag gccagtaggt aacatctgta ctcgatttgg
     3721 cctttatcga acatccaagg tcgcaaggtc cgcggttgtt gcaacctggc cgaccatcat
     3781 actcaaccgt atttattgct gaatctgaag gacaccagtg ccagcccagt tcgtcgaatc
     3841 cggcagcgat tttatcacct agtttcccta agcccacggg aggtgtttgc cggggtgatt
     3901 ttggaggata tgcaggatca ccatcgagtc ccgcaacccc catcattttg tcattcaaat
     3961 taaagtacgg ttcaagatca aaatatgata atggccaatc gtccgcaata ttatccaatg
     4021 ttttaactcg gaaatcagag ggttttagtc taggaaagtg ggcagaccag tggatagtac
     4081 tacccctgac agcgttgtac atcagaggag ctatcgggct ttccatatca tttattgggt
     4141 aatctacatc caacgatcta aaatttgggt tcggattaaa atccgtttgc ctttgtattt
     4201 cccaatcttg actggatgtt taatagtcag ttgaggggtc tatccaatca ccctgttcca
     4261 ggcacatcac tttcattcct gatttggcca gtctccaggt aaaggctgct cccgaggctc
     4321 ctgcgccaat taccaatacg tctacctttt tattgttaat gctcaatcca actctccttc
     4381 cgaccactcg gtgattgttt gggttgctca attacactga ttctaatatc tcagttacgt
     4441 gtgttgaatc taaaagcggg tatatttgct caaagataga agttatttct gtagtttgcc
     4501 taaccatagt tctggagtta ttgtaaacct caatagattc ccattcttgt gtggtcacaa
     4561 taattccatg tgatcccaaa aatttccggt aaacttttac ttctggcaat ccgacttcga
     4621 tggtcttttg acttacagtt ttcagaaggc caatcaatcc tcccgcacca ggttttggtg
     4681 tgtatgttct ctgtacgatc aacatattta ctccctgttt tttaacgcat taatattttt
     4741 cgagattatt tacagcctat tataagtttt taacaaattg aattatcatt tgcgaaccgc
     4801 tacatattta gaaaaagaat aggtacaaac ttaataatgt ctgcgcttac cgaaaaggaa
     4861 gtattagaga ttttctctgc gggagggagt cttgctggaa aagatttaac cggattgaac
     4921 ttgcacagat ccgtgctaag cggtgttgat ttgtcaggtg ctgacattcg aggtattaat
     4981 tttaggggtg ccaatttaag tgggtctaat ctatcaaatg tgaaatcttc agagggcatc
     5041 ctcgctgaag ccaacctcag ggactctgat ttaagtggtt cagatcttag tgatacgtat
     5101 ctgatggaag ctcggttgta tgcttcggac ttatctcact gtgatttgac gggttcgaac
     5161 ctgagtggag cctttatgac aggaggtgtg aacctatgta attcatccct agttagtgcc
     5221 aatttaaggg gagctgattt gtctggtgca ataattaggc aagccgacct aactatggcc
     5281 acgcttagga gtgtgaagtt gataggtgct gatgccagtt tctccgattt tacgtcagcg
     5341 gctttgaatg gggccgatat gactaagaca aatctctctg gtgccaaatt agttaatgct
     5401 gttttaaatt catccaagct agaatttgcg cgattggata acgccaacct gactcaagcc
     5461 agtttgagaa gatgcatcct cagaggcgcc agcctgcaaa tggcgaagct tgccaaaacg
     5521 aacctgatct tcactgattt tcaggaggcc gatcttacct tagcggatct tagcgagtcc
     5581 aaccttagag gggcgattct tctaggtgcc aatttgaaat ggactgactt aagcttgact
     5641 gaatttgata attcaaattg aaagatctta ccttagccgc tgtttttata tctttgttta
     5701 acggaccaat cgtctcatta ttggtactta gcgcttaaat tagaccgtct attcttcaat
     5761 tatagttacg gatttcatag cgtccccagc agtagtagcg cttccaggat ctctttcgct
     5821 tatggagaga actgtctcca tcccatctga aaccctgcca aatacgctgt ggaggccgtc
     5881 gaggtgaggg gtcggtgcga acgttatgaa aaattggctt ccattggttc ccattccgtt
     5941 tataactcca cggtttgcca tggacaatat tccgggagaa tcatgtttgg catcatcatg
     6001 gaattcatca tcaaagttat atccaggccc gccagagccg gttccagtcg gatcccctga
     6061 ctgtgccata aatccaggta ttaccctatg gaacatgatt ccatcgtaat acccgtcttt
     6121 cgccaaggaa acgaaattat ttactgtttt cggagcctct ttggataata aatcgattgt
     6181 gattttattc ccattatcca tttcgattat ggctttgtaa ttcttttctg gatctaggtt
     6241 gaattcggga ggttgactgt aggttttatc gctcattcca cgacctttat ttattgattt
     6301 gccctgttga ttcgaactgt tcgattatat ccttaatttc tgttgagacg atacgagatt
     6361 tccccaattc agaatcaaaa ttcactgaaa tggattgacc agagcagagt aattcgtctg
     6421 tgtcacttct gtaaatttct gatcttttgt caatacttgt tctcccaatt ttatccacgc
     6481 gcaaaaaaat atctataagt tcatcaattt ttgctgggct tttgtattct aaagttacct
     6541 tgacagcggc aagatcaaaa tttttacggg tatctgcttg atgggtaaga attcctaagt
     6601 ttctgaaata ttctgcttgt gcgacatcta tgaaatctat atagctgccg taatatgcga
     6661 ttccttgaat atcacattct ttccaccgca ctcgtaatgt gtgtttgaag ttgaaatcta
     6721 atttgctcaa ttgtatttat cctttagaaa tatatgtttg ttggtactgg gcaaatggat
     6781 atttgctatt ttaattcaat accacaaaag aaattaggtg aaatcaccta atttagtagg
     6841 gtgtcaacac gcatgaatta agtctatact tgtggtctaa ttcccatgta acggttttag
     6901 tttgggtaat tgtctggata tttcctgcga tcccgttcaa gaaatcatta taaagtagta
     6961 aaataaacga gatctttttc aaaaatgaga gaaattactg ggggacttta taacatgttc
     7021 aaaaaagccc tgatgaaaat gtcagttata gggattatcg gagccgcgtt tgtggcttgt
     7081 ggtgttgttg atgtggaaca ggctcaaggc gtcgtggatc tacgagccag aatactcgaa
     7141 atccaaacta aacaagttga tccactcgtg gaacagataa atgcaataga agagcaagta
     7201 gcccctattg aagcggaaat tgaggctcta gaaagacaaa aagaagaact ttacagagag
     7261 gcggaaggga taggtcagca atttgatgat gaaatgcgag agcggtttga cactacctac
     7321 atggaaggtg accaggccag gcaagaattt gaaaacgaga ttcaagaaag acatagagag
     7381 ctggaggaaa aggaaagatc cttacatgaa gagcaacaga gacttcatga tgaattagag
     7441 gaggctaatc aggagaagag gcaggctata gaagatgctg gccaagatac ctgggaagct
     7501 cttgaatttg agatgagagc taaagaagag gaggcccaga aggtatcaaa atctatgtgg
     7561 agggcctggg acgatgaaag ggatgaggct cagcagcagg tggagaattt gagaagggaa
     7621 ttcgaggaaa ataatccttt caacatggaa atggaagcca tacatcaagc ctggaaagat
     7681 attgatgatc tttatttcga gatagatgtc gagcaaatgc aaatcgaaga agcccgtatg
     7741 gaattgcacg acatgatgag acctctggaa gagcaggcgc accagctcag aagggaggaa
     7801 cgcgacatac aggaacaaga tccaaatcaa ttcggtagct ctgcaaactc tggccaagat
     7861 gcaaatcaga tgattagaga acagcatgat aaattggaag atcttcggcg ccaattacgt
     7921 gaagcaaccg atgaacaaca gcaggcacaa actgctccaa ggcaactaaa ttgggatgac
     7981 catggtagga agactgagga gatttgggct gactatgaag cacggacaga agaaatagct
     8041 cttaaaagag ctcaggcttc agataccacc ggagcggctg gagatgccag tatggctcaa
     8101 gctgctttgg atgaaatcaa tatcaatttt caagaccagt tagcattcca agaagctaaa
     8161 ttgcttcagg ctatggagag tttacaagct tttgagacag gggctgcagc caatgatggt
     8221 tcggctctaa gaagccagtt gattgcaaat attgagggaa tgcgaagcga ggttaatgaa
     8281 catcagaatg taatggattc cttgccggca aatcttcagg acgaactggg aaaccctgaa
     8341 taccataata tatcgggtca gttggcagat gcggaatcgg ccttagcaaa tacgcctgag
     8401 actaatacgg ttgacacgac gaatgccgac ggtactacta cggctcaaac ggtagagaat
     8461 ccggtttatg caggtgcttt agcgtcggtt gaaaacctga aagctcagct agctaacatt
     8521 ccagaattca tactcggtga gccttacgct aatccgtccc atcttgagcg tactatatca
     8581 attggttcac ttaaccagga aataagcatt cttgaggcag agctgaacac cctttcagat
     8641 caacccacca ctgcagaccc tgcctacacg gcggctttag cagagaagaa ttcattggac
     8701 agcctgatcc acgagacacg agagcagtgg gatgctgagg ttgcggccaa gcagaacatt
     8761 atcgacgccg ggtctggagg agcacctgat cagggtcaac tcgatgcaga aatccaggat
     8821 ctgatggcaa atgcagaggc tgatagagac aatgcactgg cagccctaga tgctgaattc
     8881 acagtcactg caggcacgaa cgcagatgac tctggaggta gggcagaaga gatacgtgac
     8941 gagatagaga atattgaaaa ctatattagc gaccttcaca actcgaagga tcaagggcat
     9001 caggcccaaa accaggcgct tgaaaatgtt agaagtgaag tgcgagagat agaagagcag
     9061 atggaaggca tgcataattt catgcgagaa ctggaagatc aacagaggcc tctgcatgag
     9121 aagaggatgg cgcttgataa agagagaatg gtgctcgatc aaaaacaaag agagattgag
     9181 gaccagcgtg gaccatggga agaggaaagg aaccgttatg aagaggatct atggtcggga
     9241 ttcgatgact ggcagcgaac tcgacagcgt gagatagagg accaacaaga agcgatgtgg
     9301 gaattagttc gagtcgatat ggaccagaat agaagggata cagaagtagg gatgaagcag
     9361 atgtggaaag attttgaaaa cgaatccaga gattctcaca aaggtgctga tcgcgaattt
     9421 aaagaaaagc gaaaggctat agatcaagag cgacaggagc ttgaagaaag ccgagacaac
     9481 ctaggacagg cttttgaagc tgagcgtgag gaagcaatac aagaaattga aaatatgcgg
     9541 gatgaactct atgaggagcg aatggcccca attgaagagg aaatcgctta tctagatgac
     9601 gagattggtg ctaagtttgc ttcaattgaa ggcttgtacc aacaacaaga ggacctggcc
     9661 ggacagcttg aagacttaga agcacaagtc agggaattgg accaggaagc tgagttcgga
     9721 ttacttagtg tgataagtgg agctatagat aacgccgaga aattggagca gaatccagct
     9781 tcattagctg gagcagcagg agtatcgctc actgacttct taccgtcacc accgtctaga
     9841 gaataaatat agaaaggcta attagtctcg ctaaatacaa attctaggag aatttgtggg
     9901 aaagggtcac caggctggat aagctggtga ccctttcttt tgttcaaata aaagaaagga
     9961 atggttaaat attgttgtta attgtaaatg ctcagcgaca acaaccactt caaatgcacc
    10021 tttaaaacac cccgctagtt gctaatataa gatttttgct gggatgtagt tattcatgaa
    10081 gtagtaaagc cttctctttc tcttcaggct catatagtta cctatcctgg aaatgggcca
    10141 ctacaaacta ccgcctcgcc cgaacatact gaggaggcca tacagaatcc tgaccatcca
    10201 actcaacctg cgcattcaac ggccagtatg ggtttctaag tagttctctt cccagaagaa
    10261 taacatctgc ctccccattt cgaagagttt gctcagcttg agaagcgtca gtaatcaacc
    10321 caactgctcc agttaatatc cctacttctc ttcttatggc agcagcaaac ggtacctgat
    10381 aaccgggaaa tacgtccaaa ttttgagatg gtgaatttcc accagatgaa caatctatca
    10441 tatcgacacc ctcctcctgt agccaccctg agagttctat ggaatcttca agcgaccatc
    10501 cgtctgacat atattccgtt gcagatattc ggaccaatag cggcatagat tctggaataa
    10561 cctttcttac tgctgagaca atatctaagg gtaatcgaca cctgtttctc aatgttcccc
    10621 cgtagctgtc ctttctattg ttggataatg gtgacatgaa ttcacaagcc aagtatccat
    10681 gagctaaatg taattccaaa actctaaatc ccgcccctac agacctaact gcagatacag
    10741 agaagtctga aatgattgaa tctatatcct ctattgttag ttcattaggt acaatccacc
    10801 cttcgtcaaa ttcaagcgaa ctaggtccca ctatgtccca accgccatcc ttttttgtta
    10861 aaacgtctcc tccttcccaa ggccttttgt gtgatgcctt cctacctgca tgagccagtt
    10921 gaatggatgg agttgctcct tgcgaatcaa taaaactggc aatcggctgt aagacagtag
    10981 cttgttcatc agaccaaata cccaaatctt gaggtgatat tcttccttcc ggggataccg
    11041 ccgtcgcctc agccataaca agtccagcac ctcctaccgc cctagtccct aaatgaacca
    11101 tatgccaatc tgtcgccaca ccatctccat tattagccga atattggcac ataggtgcta
    11161 cgaaaagcct gttccgtatt ttttcccctc ttatcgtgat aggggtaaat aaattgctca
    11221 tacgatcaaa acccttttat attttctatc ctgctcttat caatctaaat tcaacttttc
    11281 cttcttcaac caatcctttt attttccttt gtttggcaga catctgacta gaagaagatt
    11341 tgaattccac caacactatt ttgtcttctt caaactgtat tccgtcgatt ggactgccga
    11401 gaaatctaaa ctgtttgcta tcccaagggt aggcttcaac caagggtaaa aactgttcag
    11461 ttatttgtcc gtatcgggtg gactgactcc taatttgtga tttcagttcc gaggctgcag
    11521 atttatattt gaagcacata ctggtaacaa aaaataaaca cccagataaa atgagtaata
    11581 accaccacga gagtaaaaaa tccatgcgta tacctcttag ttaatgatga cgtgggctac
    11641 tacaagcagg ctaacttacc ttccaattct tttccgaatt ctagatattg ccatttcctt
    11701 acttgaggtt ctgaaatctc ttcattaaac ctacttggat ctttggatag tctgtcaagg
    11761 ctaatagttg gccacaaaag actagggtct ataccgatac tctccccctt tgtcttcctc
    11821 catcccttta actctttcaa cctggtatta gccttttctc tttccttgtt cgacattaca
    11881 tttgttcctg tgttttgcct gcggggtcgt ccaattggag gttggctgag ggcttctatc
    11941 gctatttttc taatccgttt tgacacttca gcacgacccc acataccaat acctttaatt
    12001 tcggaataat tggaataagg gtttctagca atagccacta acactgaatc ccctacaatt
    12061 ttaaaaggag gacgatctcg gcctatagcc tcatcttctc ggacagaata tagggattgc
    12121 agcactgaca aagccttacc gtctaggatt ttacttcctt taacatccaa aaaagcgatg
    12181 tcttcatctc taggactaaa ctttacaagt tgcaaccttt cacattcctc tttcaaccat
    12241 gaaatccgtc ccaggttctc gagtttgttt gtcataactt ccatagctcg gaatagatac
    12301 ctaacgtcat ccgctgcata ctttctagcc tctttgctaa gaggtcgtag gctccagtcg
    12361 ctcctctgta gctttttatc tttggtcaca tcggcatcga tatattcttt taaaattgaa
    12421 tcgaggccta atttctttga tcctagaagg gcagctgcaa ggctggtgtc aaacacattg
    12481 ttgaatgaaa aattgtaatc tctatttaga ctacgaatat cataatcacc ggcatgaagg
    12541 attttgataa ttttatcatt agcgagcacc ttgcctagag cggatatgtc atctattgct
    12601 aatggatcga caaggtatac atcttcacct acacaaagct gaatcaaaca tacaaattct
    12661 gggtatctaa aaaacccgtt cccttctatg tcaatgctta cgtaacggtt agacaaggaa
    12721 tcctcaatga ccttttctag atcatctgag gaagttagaa ttttgggtat ctcgtctatg
    12781 gctcttaaac tcatgtatga cttttatttc ttatatgaag tataacctaa tctacctttt
    12841 aatttgcaga atttttgatc tttatgtaag tttcagacct atataacgat caaggacata
    12901 aacatggtaa gaataccccc gtggagtaga gaggaaacaa tcctagcgct ttacgcgtat
    12961 atgaaccatg ggcgtttacg ggaaaatagt tttgaagcaa aggagctaag cacctatttg
    13021 cggcatctta cagaccctca tactacatct gatcctgata agtatagaaa tcctgatgcc
    13081 attgccctaa agctccgtaa atttgcagcc ttggatcc
//
LOCUS       DASCNS010000003         2315 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000040051, whole genome shotgun sequence.
ACCESSION   DASCNS010000003 DASCNS010000000
VERSION     DASCNS010000003.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000040051"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="QF838_00130"
     CDS             complement(<1..2285)
                     /locus_tag="QF838_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015155.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HJO59135.1"
                     /translation="MEKQSYLASYYDFELATQINGIISVLETIEITLANQGLYLESAS
                     EEQIKIAFHHIMSDSRLASVKDLISTQASSTKKIVLGQEIDYWVSPAMVFHVIADNSK
                     LGGAGLEIGMGGGPDVINKEHLNRSVGSVMSGSSQNYEVWGIAIDANLESLDEGKTAG
                     IFIMFTAIAAVLVVGFSLGSYWATAITGVGIAILMVWLKGISALIGLKGGLVIDLVVP
                     ISMISLGVDFIVHAVRRYREELLQGNNPKISFTSGYASVLGALLLAMASDSIAFLSNL
                     SSNIEAVIHFGCAAAIAVISSFWILGVAAPLLTMKVDQLIIQSRYDFQTTRWLTYRIL
                     GSILVASISGISIIMLVAVSRLIGLVLLGGGIFLLILVPIFILARSTSSIVFEDSKNM
                     GMAAPSQDRFAHIVARIVTFAADNSVKVIFLTVLITALSIYSALQLTPSFDVKDFFDS
                     ESEFVVGLDKFDEFIAGGEPGVTFVKGDLTDPTVYDDIKIYIEELRGINLVGETPSGD
                     VTFGLNALNVLTTIMHNPVSVASIEEATGITITDSTSNGIPDTKQQIATIFEHSLQKG
                     VWGDSQNLILRPDQVQGAVYFRRNEEALTTIQFQMPGTRDQAVVTAALKEITPSVLKL
                     ENHPSLSKVALTGSPFQREVQLSESTRTLYTSLPIAIVAAATLLLITMRSFRYAIVTV
                     IPIGLVVAWLYGVMYAFGFSLNFVTAMIGAISIGVGIDYSIHMTIRFREELNRNESRI
                     LAVQKAAGGTGVALVASAASS"
BASE COUNT          718 a          534 c          431 g          632 t
ORIGIN      
        1 atactagatg ctgctgaggc caccaaagcg acacctgttc cccctgccgc tttttgaacc
       61 gctaatattc ttgattcatt tcggtttaat tcctcccgaa accttatggt catgtggata
      121 gaataatcaa ttccaacccc tatggaaatc gcccctatca tagctgtcac aaaatttaag
      181 gagaagccaa acgcatacat aacaccataa agccaagcca caactaatcc tatgggaatt
      241 acagtgacta tcgcataccg aaaagatcgc atagttatta aaagtagtgt cgctgcagcc
      301 actatagcta ttggaagaga agtgtataag gtgcgggtag attcagataa ttgaacctca
      361 cgttggaacg gtgaaccagt caatgccact ttagacaatg atgggtggtt ttctaatttc
      421 agaactgagg gagtaatttc cttaagagca gcagtcacga cagcctgatc cctggtaccg
      481 ggcatctgaa actgaatagt tgttaatgct tcttcattcc ttctgaaata taccgcaccc
      541 tgtacctgat caggtcttag aatcaaattc tgactatccc cccacacacc cttttgtaaa
      601 gaatgctcga atatagtagc tatctgttgt ttcgtatcag ggataccgtt cgaagtggaa
      661 tcggtaattg ttattccggt agcctcctca attgaggcga cagaaactgg attgtgcatt
      721 atggtagtca aaacattgag agcatttaga ccaaaagtaa cgtctccact gggagtttca
      781 ccgactaaat taattcccct cagttcctca atatagatct ttatgtcgtc ataaacagtg
      841 ggatctgtca aatcgccctt aacaaaggta acccctggtt caccaccagc tatgaattca
      901 tcaaatttat ccaatcccac cacaaactct gattcagaat caaaaaaatc ttttacatca
      961 aaagaaggtg tcaactgtaa agcggaataa attgaaagtg cggttatcag caccgttaaa
     1021 aatattactt ttactgaatt atcagctgcg aatgtgacga tcctggcgac gatatgggca
     1081 aacctgtctt gacttggcgc tgccattccc atatttttcg agtcttcaaa aactatacta
     1141 gaagtacttc ttgctaaaat aaatattggt acgagaataa gtaaaaaaat gccccctcct
     1201 agaaggacca accctatcaa cctactgacc gccaccaaca taattatgga tattccagat
     1261 atagaggcaa caaggatgga acctaagatt cgataagtca gccatcttgt agtttgaaaa
     1321 tcatatcgac tttgaataat gagttgatcc actttcattg tgagaagtgg ggccgctaca
     1381 cccaaaatcc agaaggaaga gattactgca atggcagcag cacatccaaa gtgaattacg
     1441 gcttcaatat tggacgataa attcgagagg aaagctatac tatcgctggc catagccaga
     1501 agaagggctc cgagaactga ggcatagccc gaggtgaaac taatcttagg attattacct
     1561 tgaagcaact cctcacggta tctgcgaacc gcatgtacaa tgaaatcgac tcctaatgaa
     1621 atcatactga ttggaaccac tagatctata accagaccgc cttttagccc aattaaggct
     1681 gaaattcctt ttagccacac cattaatatg gcaatcccta ccccggtgat cgctgtagcc
     1741 caatatgaac caaggctaaa cccaactact agaactgcag caatagctgt aaacattata
     1801 aagatgccag ctgtttttcc ttcgtccagt gattccagat ttgcatctat tgcgattccc
     1861 catacttcat aattttggct ggaacccgac ataaccgaac ccacacttcg attcaaatgt
     1921 tccttattaa tgacatctgg accccctccc ataccgatct ccagcccagc tccaccaagt
     1981 ttggaattat ctgctataac gtgaaaaacc attgctggag aaacccagta gtcaatttct
     2041 tgacctagga caattttttt tgtagaagaa gcttgagtcg aaatcaaatc ttttacacta
     2101 gccaggcgag aatcagacat tatgtgatgg aaagctatct ttatttgttc ttcgctagca
     2161 gattccagat acaaaccttg attcgcaagg gtaatctcga tggtttccaa aacagaaatg
     2221 atcccattga tttgcgttgc tagctcaaag tcatagtagc tagcaaggta tgattgtttt
     2281 tccaaagttc cccttggttt accttcttct gtgac
//
LOCUS       DASCNS010000004         2085 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000044181, whole genome shotgun sequence.
ACCESSION   DASCNS010000004 DASCNS010000000
VERSION     DASCNS010000004.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000044181"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /gene="rplF"
                     /locus_tag="QF838_00135"
     CDS             <1..333
                     /gene="rplF"
                     /locus_tag="QF838_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HJO59136.1"
                     /translation="LLSNMVEGVSEGFTKELELVGVGYRVQQKGKGISLSVMLSHTVE
                     IQPDSGAELEVDGNTHIKVSGIDKQVVGQLASQIRKVRPPNVYTGKGIRYAGEVVKLK
                     PGKSARRA"
     gene            337..702
                     /gene="rplR"
                     /locus_tag="QF838_00140"
     CDS             337..702
                     /gene="rplR"
                     /locus_tag="QF838_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HJO59137.1"
                     /translation="MSTSNGKMRRVVRHKRLRSKISGTAERPRLAVYRSISHIYAQLI
                     DDVKGSTIAAASSLENGLNDKEKDAGKISISKVVGEFIGKRAAEKGISEIVFDRGGYK
                     YHGRIKAVAEGAREAGLKF"
     gene            747..1307
                     /gene="rpsE"
                     /locus_tag="QF838_00145"
     CDS             747..1307
                     /gene="rpsE"
                     /locus_tag="QF838_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HJO59138.1"
                     /translation="MQEKQDRRRNKDREEESPITERTVRINRVSKVVKGGRHLSFSAL
                     VVVGDGEGRVGIGMGKASAVPDAITKGSAYAQKNMVDIPLKGDTIAHDVSAKFGGSMV
                     MLKPAPPGTGVIAGESVRAVVELVGVKDIVTKVRRSTNPTNVVKATLKGLQMLRDPEV
                     EISRRREFAQRTALAKNETLSQDPSD"
     gene            1319..1501
                     /gene="rpmD"
                     /locus_tag="QF838_00150"
     CDS             1319..1501
                     /gene="rpmD"
                     /locus_tag="QF838_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HJO59139.1"
                     /translation="MAKISVTWKKSTIGYSQRQRRIIESLGLRKLNHTVVHESSPSIR
                     GMIKKVGHLVEVREGE"
     gene            1536..1985
                     /gene="rplO"
                     /locus_tag="QF838_00155"
     CDS             1536..1985
                     /gene="rplO"
                     /locus_tag="QF838_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HJO59140.1"
                     /translation="MQNHTIKSSGAKSGKRKRIGRGDGSGNGSFSGKGMKGQKSRSGG
                     GVRPGFEGGQLPLIKRMPSLRGFTNIFKTNYHLVNIDTICRVYPDGGEVSPQGLVQSG
                     VIKDEKLPLKILGRGEITVNLKVSAHKFTKSAKNKIETAGGTVQVID"
BASE COUNT          705 a          338 c          559 g          483 t
ORIGIN      
        1 ctgctatcaa atatggtaga aggagtgagt gaaggtttca ctaaagaatt ggaattggta
       61 ggtgttgggt acagggtgca acagaaagga aaaggaataa gtttgagtgt catgttgagc
      121 catacagtcg aaatacaacc ggactcaggt gctgaattag aagtggatgg aaacacgcac
      181 ataaaagtgt caggtattga taaacaggtt gttggtcaat tagcgtcaca gattcgtaag
      241 gtcagaccac caaacgtata tacgggtaaa ggtatcagat atgctggcga ggtggtgaag
      301 ttgaaacctg ggaagagtgc aaggagagcc taaattatgt caacttcaaa tggaaaaatg
      361 agaagggtgg ttcgtcacaa aaggctgagg tctaagatct caggtactgc cgagagaccg
      421 cgtttagcag tctatagaag catttcacat atatacgcgc agttgattga tgatgtgaaa
      481 ggaagcacga ttgcggccgc atcttctttg gaaaatggat taaatgataa agaaaaagac
      541 gcggggaaaa tatcaatatc gaaagtggtc ggagagttta ttggcaaaag agcagccgaa
      601 aaaggaataa gtgaaatagt atttgaccga ggtggatata aataccacgg aagaatcaaa
      661 gcggttgccg aaggagctcg agaagcgggg ttaaaatttt agcaaggtat attaataaaa
      721 tgtgcccaga cttaggtgtt aaatagatgc aggaaaaaca agacagaaga agaaacaaag
      781 accgggaaga agagtctcca ataacagaac gtactgttcg tattaataga gtctcaaagg
      841 tggtgaaagg tgggcgacat cttagtttca gtgcccttgt agtggttggc gatggagaag
      901 gacgagttgg gattggtatg ggaaaagcct cggctgttcc agacgctatt accaaagggt
      961 ctgcatatgc acaaaaaaac atggtggata tccccttaaa gggagatact attgctcatg
     1021 acgtttcagc taaatttggc ggcagtatgg taatgctgaa gccagcccct cctggtacag
     1081 gtgtaatagc gggagaatcc gtaagagcgg ttgttgagtt ggtgggagtg aaggatatcg
     1141 ttactaaagt gaggcgaagc acgaatccta ctaacgttgt taaagcaacc cttaaggggc
     1201 tccaaatgct gagggaccct gaagttgaaa tctctcggag gcgggagttc gcgcagcgaa
     1261 ctgcactagc aaaaaatgaa actctttcac aggacccatc agattaaggt atcgcaaaat
     1321 ggcaaaaatt tcggttacat ggaaaaaaag caccatcggt tattcccaaa ggcaaagaag
     1381 aataatagag agtttgggac ttagaaaact aaaccataca gtggtacatg aatcatcccc
     1441 ttctataagg ggaatgataa agaaagtagg gcatttagtc gaagtcagag agggcgaata
     1501 acagggactg ataatttagg taattagata tgactatgca aaatcacaca ataaaatcct
     1561 caggagccaa atctgggaag cgaaaacgaa ttggccgagg tgatggcagt ggaaatggaa
     1621 gtttttctgg taaagggatg aaaggtcaga aatccagaag tggtggtggt gttagacctg
     1681 ggttcgaagg tggtcaacta cctttgatca aaagaatgcc gtcactacgg ggtttcacaa
     1741 atatttttaa aactaactat catctagtaa atattgatac catctgccgt gtgtacccgg
     1801 acggggggga agtatccccg caaggattgg ttcaaagtgg agttatcaaa gatgagaaat
     1861 tacccctgaa aattttaggt aggggcgaaa taacagtaaa tttgaaggtc tctgctcata
     1921 aattcacgaa atctgctaag aacaaaattg agacggcagg tgggacagta caggtaatag
     1981 actagatggt tcggcaagct caatcgtctc gatctgacca attatcacga cctcaattaa
     2041 tccagtccat gctggatgca atgaaggttc cggatttacg gtata
//
LOCUS       DASCNS010000005         4938 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000058682, whole genome shotgun sequence.
ACCESSION   DASCNS010000005 DASCNS010000000
VERSION     DASCNS010000005.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000058682"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="QF838_00160"
     CDS             <1..408
                     /locus_tag="QF838_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HJO59141.1"
                     /translation="SIYLLVMACAVLCMPTGLSAIRSSMTQISPRYEESSMTLGKSRI
                     KSVITTNVPLLKSGVTMASVTVFLITMKELPAVLLLAPLDFHTLTTAIWSFTSEAFFA
                     RAAIPSLLLILLSSIPLTVIVLKNNIFNNSWGE"
     gene            413..1468
                     /locus_tag="QF838_00165"
     CDS             413..1468
                     /locus_tag="QF838_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO59142.1"
                     /translation="MVDALLKCSDLNIKYGQNHVIKNLNMHLLAGEILSILGPSGCGK
                     TTLLRIIAGLEIPSQGSIFLKNHEIFGPNINLQPSNRNIGMIFQDYALFPHLTVEKNI
                     LFGISNLEQRIQDDNLERVQQLTRLKGLMDRYPHQLSGGQQQRVAIARTLAPSPTVLL
                     MDEPFSNLDASLRLSVRMEMEQILKSSNTATIFVTHDREEAFSISDRVAIMVDGAIQQ
                     VGPPDQIYFWPNSKESALMSGSCDFIKGSVSGGSVNTSIGPLPMRIPEAFSDGDEVEV
                     AIRQTDLSMEPTPTGKNIVVRKDFRGDETIFWVKTPQEEVIQCKHKIHTTLFPGLKVQ
                     LSPVQYTKFNVFNVPRS"
     gene            1570..3054
                     /gene="gatB"
                     /locus_tag="QF838_00170"
     CDS             1570..3054
                     /gene="gatB"
                     /locus_tag="QF838_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HJO59143.1"
                     /translation="MTDKYETVIGLEVHSQLLTLSKMFCPCGSDYQDSEPNTRVCPVC
                     MGMPGVLPVVNKKAVNLVIKTGLALECEISPETKFDRKNYPYPDLMKGYQISQFDIPI
                     ALGGRLSIKTPETGGKYIRITRVHLEEDVAKLVHRKEPNGETYSLLDINRAGVPLMEI
                     VSEPDMNSPEEANEYLTNLHSIIRFVETSTANMEEGSFRCDANISVRPLGSKKLGSKV
                     EIKNVNSFRSVFRALKYEETRQRKLLDAGHNIEQETRGWSDDRGTTLSQRTKEYAADY
                     RYFPEPDLLPILISNEWVKSIQSEMPELPAKKKERYIDLFGLSDYDASVMIGDKESTQ
                     LFENTLPYLSESMDQKDAAKATANWINVDLNRMKNELKYNWPEIKLNPKEFAKLIILV
                     DDGKISNSMAKTVFDDMFVNGGDPIKISEDKGLSQINDASSIQNVVTETLLENQSAVD
                     DYLNGKDSAVRYLVGQVMKKTKGQANPQVASKMVTSALIKQKTQ"
     gene            3089..4030
                     /locus_tag="QF838_00175"
     CDS             3089..4030
                     /locus_tag="QF838_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HJO59144.1"
                     /translation="MNDSSKTKTPHIVAIGGGTGLSVLLKGLKQHTQRITAIIGVADD
                     GGSSGRLRREMGIIPPGDFRNCIVALSDEDSLLKELFQYRFPEGSELQGHSFGNLFIA
                     AMTDVTNSFEDALTESSRILSVTGRVLPATIENVNLSVKLADGRFIKGESNVKESSSE
                     IKELFIDPPDAEASPAAIKAIFGADLIVIGPGSLYTSILPNLMVPGIVDAISGSKGIK
                     YYICNVATEIGETQHFNVVKHIDVLESYLGPGILDMIIANDNIGSIGKQFRGEAVDPN
                     TGSTKHLIVTSDLLDEKHKVRHDSDKLAKFIMRNYLG"
     gene            4072..4290
                     /gene="secG"
                     /locus_tag="QF838_00180"
     CDS             4072..4290
                     /gene="secG"
                     /locus_tag="QF838_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HJO59145.1"
                     /translation="MDTAINIIMISISLLLIAVILMQVKGQGTALFGAAESSFRTRRG
                     IELALFRFTIALVVVFIAVSIISASSWF"
     gene            complement(4312..>4938)
                     /locus_tag="QF838_00185"
     CDS             complement(4312..>4938)
                     /locus_tag="QF838_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013565.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HJO59146.1"
                     /translation="VEPFARYWMHNGTLGYGQEKMSKSLGNVFSIAEALEEFSSDALR
                     MFFLSSHYRTPLVFSNESVKGQQRAMERLKSALESPSGEGTVLDTKTYQNRFKSAMDE
                     DFNTPIAMSVIFDLARDINRESTKGLNVEAAQELLGDLAEVLGLTLKSDDSEFEGNLA
                     PFIDLLLDVREGLRKEKQFELSDRIRDRLDDLGVSIEDTTTGVRWKLD"
BASE COUNT         1588 a          991 c          993 g         1366 t
ORIGIN      
        1 tcaatatatt tattggtgat ggcctgcgct gtcttgtgta tgcctacagg gttgagtgca
       61 ataaggtcat ctatgaccca aataagccca aggtatgagg agtcatcaat gaccctcggt
      121 aaaagccgta ttaaatctgt cattacgacc aatgtgcctc tccttaaatc tggagttaca
      181 atggcttctg tgactgtatt tttaataacg atgaaggagc taccagcggt tcttctacta
      241 gcccctttgg attttcatac cctcactact gcgatttggt catttacttc agaagcattt
      301 ttcgcaagag ccgctatacc ttcacttttg ctaattttac tatcatcgat accgctaact
      361 gtaatagttt tgaaaaacaa catcttcaat aattcatggg gtgaatagat aattggtcga
      421 tgctcttttg aaatgtagtg atctgaacat taaatatggg cagaatcacg ttataaaaaa
      481 cctaaatatg catttgttgg caggtgaaat actttctata cttggtccca gcgggtgcgg
      541 caaaaccaca cttcttagaa taatcgcagg tctagaaatt ccttcccagg gaagcatttt
      601 tctcaaaaat catgaaatct ttgggccaaa tattaattta caaccatcaa atagaaacat
      661 agggatgata ttccaagatt acgccttgtt tcctcattta actgtggaaa aaaatatact
      721 gttcggtatt tcaaatctcg aacagagaat acaagatgat aatttagaaa gggttcaaca
      781 gttaacccgg ctcaagggac taatggatag gtatccacat caactatcag gtggtcaaca
      841 acaaagagtc gccattgcca ggaccctagc tcccagccct actgttttgc tgatggatga
      901 acctttctct aatctcgatg cttccttacg tttatcggta agaatggaaa tggaacaaat
      961 cctaaaatct agtaatacgg ctacgatatt cgtaacccac gatagagaag aggctttttc
     1021 tatttcagat cgggtagcta ttatggttga tggggccatc cagcaagtgg gacctcccga
     1081 tcaaatatat ttctggccca atagtaaaga gtctgccttg atgagtggaa gctgtgattt
     1141 tataaaaggc tccgtctctg gagggtctgt taacacaagc atcggccccc tacccatgag
     1201 aatacctgaa gcgttttccg atggagatga agtagaggtg gcgataagac agacagatct
     1261 ttctatggaa ccaacaccaa ccggcaaaaa tatagtggtg cggaaagatt tcagaggaga
     1321 cgaaacaata ttctgggtca aaacccccca ggaagaggtc attcaatgta aacacaaaat
     1381 acacaccact ctcttccctg gattaaaagt acaattgtct ccggtacaat atacaaaatt
     1441 caatgttttt aacgtccctc gctcctgaag tgtagtatca cattaggttt aatattaatg
     1501 cctttctaag aatttcggtt acgttgtaaa ataaacaaac gtctagtcag ggactggtat
     1561 acaaaaatta tgaccgacaa gtatgaaaca gtaattggtc tggaggttca ttctcaactt
     1621 cttaccttga gcaaaatgtt ctgcccttgt ggttcggatt accaagattc agaaccaaat
     1681 acccgagtct gtcccgtttg catgggtatg cccggagttc taccagtagt gaacaagaaa
     1741 gcggttaatc tagtaattaa aactgggctg gccttagaat gcgagatttc gccagaaaca
     1801 aaatttgacc gaaaaaacta cccatatcct gaccttatga agggatatca aatatctcag
     1861 tttgatattc ctatagccct cggtggaaga ttgtcgataa agactccaga gacggggggg
     1921 aaatacattc gaataacccg agtacatttg gaagaagatg tggctaaact agtacaccga
     1981 aaggaaccga atggagaaac atacagcctg cttgatatta acagagccgg ggttcctttg
     2041 atggaaattg tgagtgaacc agatatgaac agtcctgagg aggcaaacga atatttaact
     2101 aacctacatt cgataataag gttcgtggag acaagcactg caaatatgga ggaaggcagc
     2161 tttaggtgcg atgccaacat tagtgtgaga cctcttggtt caaagaaact tggttctaaa
     2221 gtggaaatta aaaatgtcaa tagtttcaga tctgtattta gggctctgaa atatgaggag
     2281 actagacagc gaaaattatt ggatgcaggc cacaatattg aacaagagac tcgcgggtgg
     2341 tcagacgata gaggtacgac cctgagccaa cgtaccaaag aatacgctgc tgactacaga
     2401 tattttcctg aacctgactt gcttccaata ttaatctcaa atgaatgggt aaaaagtatc
     2461 cagtctgaga tgcccgagct tcccgctaag aaaaaagagc gatatattga tctattcggt
     2521 ttatctgatt acgatgcctc ggtcatgatt ggtgataaag aatcaaccca actatttgaa
     2581 aacaccttac cctacctctc tgaatcgatg gatcagaagg atgcggcaaa ggcgacagcc
     2641 aattggataa atgttgattt aaacaggatg aaaaatgaac tcaaatataa ttggcccgaa
     2701 ataaagctta acccaaaaga attcgccaag ttaattatac ttgtagacga tggcaaaata
     2761 agtaattcga tggcgaaaac tgtatttgac gatatgtttg tcaatggtgg tgatccaata
     2821 aaaatttcag aggataaggg cttatctcaa ataaatgatg cttcctccat tcaaaatgta
     2881 gttacagaaa cacttcttga aaaccaaagt gctgttgacg attatttaaa cggaaaagat
     2941 tccgcagtga gatatttggt aggccaggtc atgaaaaaaa ccaagggtca agctaaccca
     3001 caagttgctt cgaaaatggt tacctcagca ctaattaaac aaaaaaccca gtaagtttag
     3061 ttattccgat cttaattcaa aattaattat gaacgacagc tccaaaacta aaacccctca
     3121 tatagtagca ataggcggtg gcacaggact gtcggtatta ttgaaaggac tcaaacaaca
     3181 cacccaacga ataactgcaa taattggggt tgcagacgat gggggcagtt ctggaagatt
     3241 acgtcgggaa atgggtatta ttccacccgg cgacttcaga aactgtatcg ttgcgttatc
     3301 agacgaagat tccttactaa aagaattatt tcaataccga tttccagaag gatctgaact
     3361 gcaagggcat agttttggca atctttttat agccgcaatg acagatgtaa ctaatagttt
     3421 tgaggatgct ctcacagagt ccagtagaat attatcagtt accggtcggg tactcccagc
     3481 aacaatagaa aatgtgaatt tatccgtgaa gttagccgat ggccgattca taaaagggga
     3541 atcaaacgtc aaagaaagtt catctgaaat caaagagtta tttatagacc cgcctgatgc
     3601 tgaagcctca ccggcagcta ttaaagccat atttggagca gatttgatag tcataggccc
     3661 cggaagctta tatacaagta tattgcccaa tttgatggta ccgggaattg tagatgcaat
     3721 ctccggatca aaaggtatta aatattatat ttgtaatgta gcgaccgaaa tcggggaaac
     3781 ccagcatttc aacgttgtaa aacacataga tgtgctagaa agttatctgg ggccaggtat
     3841 tctggatatg ataatagcca atgataatat tggtagcatc ggtaaacaat tcagaggtga
     3901 ggctgttgac ccaaatactg gctcaactaa gcatttaatt gtaacttctg atttattgga
     3961 cgaaaaacat aaagttagac acgattcaga taaattagca aaattcataa tgagaaatta
     4021 cttagggtaa tgtcctcgca acatcctcca aagtaggagt ataattactg aatggatacc
     4081 gcaataaaca taataatgat ttctatatct ctactgctga ttgccgtgat acttatgcag
     4141 gtaaaaggtc aagggactgc tttattcggg gctgccgaat cttcttttag aacaaggaga
     4201 ggcatagaac tagcattatt tcgatttacc atcgcgttag ttgtcgtctt catcgccgtt
     4261 tccatcatca gcgccagcag ttggttctaa cacccctaac tgctcacaat tttaatccaa
     4321 tttccaccga accccggttg tcgtatcttc tattgatacc cctaggtcgt ccaacctatc
     4381 tctaatacgg tccgataatt caaattgttt ttcttttcta agtccttctc gaacatccaa
     4441 caacaaatca ataaacggtg ctaaattccc ctcaaattcc gaatcatcac tttttaaggt
     4501 caatcccaaa acttcagcca aatcaccaag taattcctgg gccgcttcta catttaatcc
     4561 cttggtagac tctctattaa tgtctctggc aagatcaaaa atcaccgaca tagcaattgg
     4621 agtgttgaaa tcctcatcca tcgctgattt aaatcgattc tggtaggtct ttgtatcaag
     4681 aacagttcct tcaccagaag gcgactccaa ggcagatttc aatctttcca tggcccgttg
     4741 ttgccctttg acagattcat tgctgaacac taatggcgtc ctgtagtgtg aacttaagaa
     4801 aaacattcta agggcgtcag aggagaattc ttctaaggct tctgcaatgg aaaatacatt
     4861 ccctaaagat ttgctcattt tttcttgacc atagcctaaa gtaccattat gcatccaata
     4921 tcgggcaaaa ggttcaac
//
LOCUS       DASCNS010000006         3928 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000067768, whole genome shotgun sequence.
ACCESSION   DASCNS010000006 DASCNS010000000
VERSION     DASCNS010000006.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000067768"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            85..3837
                     /locus_tag="QF838_00190"
     CDS             85..3837
                     /locus_tag="QF838_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HJO59147.1"
                     /translation="MSINTGEVLANVPINIVMNLGDIATAVPTITVADVVTPTSTVSA
                     GATSNSIVEPVNSRSGSDAIDPLIKYSPVSGEKIVDQSILNQYSPDNYLHFDSFDFHG
                     EYQGNNANTSTYNQNLIPKPDPTDTISLPSDFPILGNVFTIEVQVYSSVDHPNMAHRT
                     IIGNDANPKKNDRVRPPTITINKENGINQIRYGFGVGPDSKGKRRVIDTDMAENQWYH
                     IAFTFDGTTTKLFIDGVEIDSSDFASGLTPHPVPLSIIGRQFLGKMDEVRIWSIARTQ
                     EDIQSTLTGELIGNEPGLIAYYPMDVDQNWELIDRGPNNHHATITNVEILQRHSSLDC
                     PNANGTLACPYPTIREALDTAQAGDSIFIKDGRYPEVIFKELFNQSHETNGPKVTLRG
                     ESTNVILDGTVPLIANWQLVNGRYEASVDMNQLSNAAGTKVENIYALWVDDRYMIPAM
                     PVNFTNPTDVSTSTQNNPEIGTVFDLNLTSPYYLPGEQTLEMQDEYIVGDINNLDAIE
                     EWSFDEVNKKLYLIPADKIPDSENVRVRVRTKIVNIEFSDNLEFRNLHFFAGAFNFLQ
                     SSYILFEDSKFSHSWEAGISYLRPGRDEGWMRGNTFKRGTNNIVRNAIFQYINDARAL
                     KFSGSMYPLVENVLFQYNDWFKNTVWSPTSNDNFRGGNKWHDETSIVGGSTWRYVTSQ
                     QNHTGGINPGLNSLVEYARIQDQYINIDGSGIQRTRGNASNSTTRYSWLLNNQRNGMR
                     LDSSCGGTDAVIHNVVSAGNKLAFRLKGDRHRAFHLLGYDTNQNDLSMPRNKYCGDDY
                     VNDTSETMAGNLNSRLLNSVAEKNISAHTPDAGDPNITLGQGDLIAENTSNEFLLNQS
                     GLWYGRALDEDKIAPYTYPHFELQDPWVENRERSDTSLQSQFGLNPFTGGVQGYDFRP
                     RKGSPLVDGGVVISGINNGEDIDPNKAPNHPPSYLGQHRKFVGEAPDIGAYEYGDSVY
                     WIPGYRSYYPTVPIPGNGAVDVPVEYGLAFNYPWKTDYSGITAEVTINGPGLNKTVSL
                     TYPNNVVFETFLPGQTYTWSVEVGEISSQIWSFTVANKIHPLNDRSVDVDDDEKLIPN
                     HNKSLSLSDGVLSFMRFDIPPSINSDYQVFLNLTPETVNSSNGQIMLYKYNYQGWGEK
                     LDSNNLGLLDHSLLTALQSISTIDASSSLSVEITDYIESTGEISFALGVLDPNDQLSF
                     FSKEKMVTDGVDIYVTAGDLLGPSGSGSGYAPQADVWPSISFVKNN"
BASE COUNT         1215 a          763 c          834 g         1116 t
ORIGIN      
        1 gtatctgtga tgcaggggac gggtgagatc cttgcgaacc acacctttac ttcaggcgga
       61 tctttctatg gagagatcag gcttgtgagt ataaataccg gtgaggtgct ggcaaacgtg
      121 ccaataaaca tagtgatgaa ccttggtgac atagcaactg ccgtacccac cattacagtg
      181 gctgacgttg taactccgac ttcgacggtc tctgctggag cgacctcaaa ttcgattgtt
      241 gagcctgtaa acagtagaag tggtagtgat gcaattgatc ctcttatcaa atattctcct
      301 gtatccgggg agaaaattgt tgaccaaagt attttaaatc aatatagccc agacaattac
      361 ctacattttg actcatttga tttccatgga gaataccagg gtaataatgc gaatacatca
      421 acttataatc aaaatttaat acctaagcct gatcctaccg acactatttc tcttccaagt
      481 gattttccaa tcctaggaaa tgtcttcact attgaagttc aagtctactc atcagttgac
      541 cacccaaata tggctcatag aacaatcatt ggtaatgatg ccaatcctaa aaaaaacgac
      601 agggtcagac ccccaactat tacgattaat aaggaaaatg ggataaacca aatcagatat
      661 ggattcggcg ttggacctga ttccaaagga aaacgaaggg ttattgatac tgatatggct
      721 gaaaaccagt ggtatcacat cgcttttacc tttgatggca ccaccaccaa attattcatt
      781 gatggcgtag aaattgacag ctctgatttt gcatcaggtt taacaccaca tccagtacct
      841 ctttcaatta ttggtaggca atttttaggg aaaatggatg aagtccgtat ttggagcata
      901 gccagaactc aagaggatat tcaatccaca ttgacaggtg aactcattgg taacgaaccg
      961 ggactgattg cctattatcc gatggacgtt gatcaaaatt gggaattaat tgatagaggc
     1021 ccaaataatc atcacgccac tattacaaat gtcgaaattc tccagaggca cagctcatta
     1081 gattgcccta acgccaatgg tactttggcc tgtccatatc caacgataag agaagcattg
     1141 gatactgctc aagcaggaga tagcatattt ataaaagatg gtagatatcc tgaggtaatc
     1201 ttcaaagaat tattcaatca atctcacgag acaaatggtc caaaagttac gctcaggggt
     1261 gaaagtacaa atgtcatatt ggacggaact gtccccttaa tagcaaactg gcaactcgtg
     1321 aacggtagat atgaggcttc agttgacatg aaccaactgt caaatgcggc cggcacaaaa
     1381 gttgaaaaca tttatgcttt gtgggtagat gacaggtata tgattccggc aatgcctgtt
     1441 aatttcacca accctacaga tgtttcaaca agcactcaaa acaatccgga aattggcact
     1501 gtatttgatt taaatctaac ttctccttat tacctaccag gagaacagac tttagaaatg
     1561 caagatgagt atatcgttgg cgatataaat aacttggatg ctattgagga atggtcattt
     1621 gatgaagtaa ataagaagtt gtatttgatt ccagcagata agattccgga ttcagaaaat
     1681 gtgcgagtaa gggttcgtac aaaaattgtc aatatagaat tttcggataa tctggaattc
     1741 cgtaatttac atttttttgc cggcgcattt aattttttac agtctagcta tatcctcttt
     1801 gaagattcta agttctccca tagttgggaa gccggaatca gttaccttcg cccaggacgc
     1861 gatgagggct ggatgcgagg gaatactttt aagcgtggaa caaataacat cgtaagaaat
     1921 gctatttttc aatatataaa tgatgcaaga gctcttaagt tcagcgggtc gatgtatcca
     1981 ttagttgaga acgttttatt tcaatataac gactggttta agaacacagt atggtcccct
     2041 acctcgaatg ataatttcag aggaggcaac aaatggcatg acgaaacaag tattgtcggt
     2101 gggagtactt ggcgatatgt cacgagccaa caaaatcata ccggcggcat taatccagga
     2161 ttaaactcac tggttgagta tgcaagaatc caagaccaat atatcaatat cgatgggagt
     2221 ggaattcaga gaaccagagg caatgcatcg aattcaacga ctcgatatag ctggctttta
     2281 aataatcagc gcaacggaat gagacttgat agcagttgcg gtgggactga tgctgttata
     2341 cataatgtgg tatcggcagg gaacaaatta gccttcagat taaaaggcga tagacacaga
     2401 gcattccatt tacttggcta tgataccaat caaaatgatc tttctatgcc tagaaataaa
     2461 tattgcggag atgactatgt aaatgacact tctgaaacta tggcaggcaa tttaaattct
     2521 cgcctactta attcagttgc agaaaagaat atatcggcac atacccctga cgccggtgat
     2581 ccgaatatca ctctaggcca aggcgatttg attgcggaga ataccagtaa tgaatttcta
     2641 cttaaccagt ctgggctttg gtacggaagg gcattggatg aagataaaat agctccttat
     2701 acctatcctc attttgaact acaagatccg tgggttgaaa atcgagaaag atccgataca
     2761 tccttgcaat cacagtttgg attaaatcca tttacagggg gtgtccaagg ttatgatttt
     2821 aggcctagaa agggttcacc gttagtagat ggcggtgtag taatttcagg tattaataat
     2881 ggagaggata ttgacccaaa taaggctcct aaccatcctc catcttatct tggacagcac
     2941 agaaaattcg taggtgaagc accagatata ggtgcctatg agtatggcga ttctgtctac
     3001 tggattccag gatacagatc ttattatcct actgtcccta ttcccgggaa tggggcagtt
     3061 gatgttcctg tggaatatgg tctggcattt aattacccct ggaaaacaga ttattcaggt
     3121 attacagctg aagtcacaat aaatggtcca ggactaaata agaccgtctc tttgacttat
     3181 ccaaataatg tggtttttga aacgtttctg ccaggccaaa cttatacttg gtcggtcgaa
     3241 gttggtgaga ttagcagcca aatatggagc ttcacggtag ctaataaaat tcacccacta
     3301 aatgataggt ctgttgatgt tgatgatgat gaaaaactca ttccaaacca taataaaagt
     3361 ctgagtttgt cggatggggt tttgtctttc atgagatttg atatcccacc ttctattaac
     3421 tctgactacc aggtttttct gaacttaacc ccagaaactg taaactcatc aaatggtcaa
     3481 attatgttat ataaatacaa ttatcagggt tggggagaga agttagattc aaataattta
     3541 ggtttattag atcacagttt attgacagct ttacagagca tttccacgat tgatgcatcc
     3601 tcctccttgt cagtggaaat cacggattat attgaatcaa ccggagaaat ttcgttcgct
     3661 ttaggagtat tggatcccaa tgatcaattg tcctttttca gtaaggaaaa aatggttact
     3721 gatggagttg acatttatgt cacggctggt gaccttcttg ggccgtccgg cagtggaagc
     3781 gggtatgcac cccaggccga tgtgtggcct agcatatcgt tcgtgaaaaa caattagtgc
     3841 agctaacacc gggcagttga ttcgccaaca cctcctgcta tttctgccat actgcgatct
     3901 cctactgtct tatcccactt ttcaggcg
//
LOCUS       DASCNS010000007         5973 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000069018, whole genome shotgun sequence.
ACCESSION   DASCNS010000007 DASCNS010000000
VERSION     DASCNS010000007.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000069018"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /gene="hflX"
                     /locus_tag="QF838_00195"
     CDS             <1..565
                     /gene="hflX"
                     /locus_tag="QF838_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HJO59148.1"
                     /translation="QHRQRYRQRRKRSNLPIVSLVGYTNAGKSTLLNTLASSAVTAEN
                     KLFSTLDPITRKISIRNGKEILLTDTVGFIQKLPTSLIAAFRATLEEAEEADLLIHVS
                     DISHPNRSEHCHAVNEVLGNLGLTDKPKIGVLNKIDLFPKNYQAHEDTSLIKLMDQFD
                     QTVFCSAETGFGIGELINVIECKISEY"
     gene            complement(614..1630)
                     /locus_tag="QF838_00200"
     CDS             complement(614..1630)
                     /locus_tag="QF838_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59149.1"
                     /translation="MNKDGFRRTRRVRGYPAHTIKDTLEIAKAIYHDNAGLPLDRKLL
                     ANHLSISPRSSSFTTLLAASEQYGFTKGRYRDETISLTDLGISISIPKSIEELAESLL
                     KAAYMPEKFRKLNKLLNEGELPKPEFLTNLLVRDLGIAIDQTDEFTRLFTENLSYIED
                     LGVRENAVKGLTSPLTDKTLPNIVENIPVSIDSVIKRPSSMAAKEGYLLFIETTETAK
                     NITSIVISQFRSLGIPTKSIGPYDDVDEPEKAVATVLVLNASEFNEEIQLTEIFAVGV
                     ANGITPGNVILVSESIEFQKYFPASALRQGQLITYQDVNSLVIGLLMHLTTKGSLSVK
                     ISIP"
     gene            1819..3060
                     /locus_tag="QF838_00205"
     CDS             1819..3060
                     /locus_tag="QF838_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HJO59150.1"
                     /translation="MTKLLTLLCVVSFISIPLACSSDTTAEPDVPTPTKYSDSSAPYP
                     MASTIPKEQLGSSEGTPRELEAVWEAWSLLNKEHIDRETFEAGTFEESAIKGLIDSVE
                     DTHTSYVDPLVLQIEQTDLSGQFEGIGAHVRRREDGMIQIISPIEGGPAEAAGILAGD
                     IILAVDGESIEGLELLEAVSKIRGPKGSIVSLTVKHIGELETTIIDVSRDVIALPSVL
                     LRSDPGDDITHIRITEFKGDTPVRLSEILNKELEAGSKGLILDLRSNPGGYLQQVFDI
                     ANMFLDDGVILLEERHTENVTWQASGGGFASTLPMVVLVNKYSASGSEILMGAFQDNG
                     RATVIGEQTFGKGTVNVFRKLSNGGGLYMSVGRWYTPNKRPIEGEGLTPDILVTSRDS
                     KEADIKQVDEAYKEINKLLND"
     gene            3073..3528
                     /gene="smpB"
                     /locus_tag="QF838_00210"
     CDS             3073..3528
                     /gene="smpB"
                     /locus_tag="QF838_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HJO59151.1"
                     /translation="MSDTLIASNRRAYYDYEILETIEAGLVLQSSEIKSIRDHRVNLA
                     GSYAFPSNGELWLNNAHIAQYPYSRGENHHPLRSRKLLLRKQELRKFMAAAQQKGYSI
                     VPLKLYLSGHFAKISIGIGRGKRQYDKRKAIIEKERSRDAQEAIRRELQ"
     gene            complement(3581..3811)
                     /locus_tag="QF838_00215"
     CDS             complement(3581..3811)
                     /locus_tag="QF838_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59152.1"
                     /translation="MATRKTKKMIAVEERFRQPLEKMLPEMVTEQGLTATADYLGVSK
                     ATLAYWLLKFRIDVRRVALAPGDTMHVTRLDG"
     gene            4053..4128
                     /locus_tag="QF838_00220"
     tRNA            4053..4128
                     /locus_tag="QF838_00220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4086..4088,aa:Arg,seq:tct)
     gene            4157..>5973
                     /locus_tag="QF838_00225"
     CDS             4157..>5973
                     /locus_tag="QF838_00225"
                     /inference="COORDINATES: protein motif:HMM:NF015168.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HJO59153.1"
                     /translation="MPPLPHRKIAEPHMTNRLSEESSPYLLQHKDNPVNWFAWGLDAF
                     EEAKARDVPVLLSVGYSSCHWCHVMEYESFENEEIAKAMNDDFVSVKVDREERPDIDS
                     IYMSAVQSMTGQGGWPLTVFLTSEGLPFFGGTYYPPEPRGGMPGFIQILLAISDAYKN
                     RRPEIEESGSYLLEHIRASITETPATNNFEPSLLEQAYEQLLAQCDWEWGGFGNSIKF
                     PQSMALEFLLRYHLNHPDSRALEMVETALQKMASGGMYDHLGGGFHRYSTDKKWLVPH
                     FEKMLYDNALLSRLYIHAFQVTGKNFYLRVAKDIYEYIDRDMTDENGLFYSAEDADSE
                     GEEGTFYLWDPNEFKRHLTDLEMALFKSAFSLGEEGDFEGKNILHYKPKGMDFGFLGS
                     ENPDLLNLRKKLVDIRNSRPKPFKDNKSLLSWNALMSLSFIEGYLVSKEEKLLEIAKL
                     NINSLLDLIEFNSGLFHSSKGGQIGSYGYLEDYSSLILAMLAMHEATLDLEWLAKTEK
                     IADTLVSLFWNSHETRLYDTPEDGDDLIIRPSESYDNATPSGSSMTAEALFKLSLITN
                     KLEYFNIFISLMSSTATIASEHPVSFGNWLCVYDSYIYPT"
BASE COUNT         1795 a         1245 c         1265 g         1668 t
ORIGIN      
        1 acagcataga caaagatatc ggcagcgaag aaagcgatct aacttaccta tagtgtcctt
       61 ggtgggttac acgaatgctg ggaaaagcac cttattaaat acattggcct cctcagcagt
      121 cacagccgaa aataaactat tttcaaccct agaccctatt acaaggaaga tttccataag
      181 gaatggaaaa gagatacttt tgacagacac tgtgggtttc atacaaaaac ttccaacaag
      241 tcttattgcg gccttccggg cgaccttaga ggaagctgaa gaagccgacc ttttgattca
      301 tgtttctgat atttcacacc caaacaggtc tgaacattgt catgccgtaa atgaagtcct
      361 cgggaatcta ggtttaacag acaaacccaa aattggagtt ttaaacaaaa ttgatctctt
      421 tccaaagaac tatcaggcgc atgaagatac atctctcata aaacttatgg atcagtttga
      481 tcaaaccgtg ttctgctcag ctgaaacagg atttggaata ggggaactaa ttaacgtgat
      541 tgaatgcaaa atatctgagt attagtctta tagccacata tttcttattg cgcactataa
      601 atgttatgta aagttagggg atcgaaattt tcactgataa gctgccttta gtggttaaat
      661 gcatcaataa tcctatcacc aagctgttca cgtcctggta tgtaatcaat tgaccttgcc
      721 ttaaagcgga tgccgggaaa tatttctgaa attctataga ttcagaaact aaaataacgt
      781 tcccaggtgt aattccatta gctaccccga ctgcaaatat ttcagtcaat tgaatttcct
      841 cattaaattc agaggcattt aaaaccaata ctgtagccac ggccttttca ggttcatcta
      901 catcatcata tggcccgata cttttagttg gtataccgag gcttctaaat tgggaaatca
      961 caattgaggt aatatttttt gccgtctctg ttgtctcaat aaacagcaga tatccttctt
     1021 tcgcagccat tgaggaaggg cgtttaatta ctgagtcaat ggaaacaggt atattttcaa
     1081 ctatatttgg tagggtttta tctgttagag gtgaagtaag tccttttact gcattttctc
     1141 ttacccctaa atcttctata tatgacaggt tttctgtaaa caaacgggtg aactcatcag
     1201 tttgatcaat agcaatacct agatctctta ccaataaatt agtcagaaat tctggtttag
     1261 gaagttctcc ttcatttagg agtttgttta gttttcggaa tttttcaggc atataggcag
     1321 cttttaaaag agattctgca agttcttcaa tcgacttggg gattgaaatt gaaatcccta
     1381 ggtcagtcaa ggaaatggtc tcgtccctat atctcccttt agtgaatcca tattgctctg
     1441 aagctgctaa gagagtggtg aaactactac ttctagggct gatgctcaaa tggttagcca
     1501 aaagcttcct atctaaaggc aatccggcat tatcgtgata tatcgctttg gcgatttcca
     1561 acgtgtcctt tattgtatgg gccggataac cccgaaccct gcgagtcctg cgaaatccat
     1621 ctttattcat gctatttcaa acttcagcct cttttttgaa atcattttga aataaatata
     1681 cggatcttgc aaaaaatgtc aatcgtcttt ttggtttttt taaccttaac gtctatttcg
     1741 tttcgttaca tattgataaa atagagtctg gatgtattct taactttaat tattttacta
     1801 ggaccaaata gaacaaaaat gacaaaatta ctcactttac tatgtgtagt gtctttcatt
     1861 tcaatccccc tagcgtgttc ttcagataca accgccgaac cagatgtacc gacaccaacc
     1921 aaatactctg atagtagtgc cccctacccg atggcctcta cgattcccaa ggagcaatta
     1981 ggctcctctg aggggacacc cagggaacta gaagcagtat gggaggcctg gtccctttta
     2041 aacaaggaac atattgaccg agaaactttt gaggcgggaa cctttgaaga atccgcaatc
     2101 aaaggattga ttgattctgt tgaagacacc catacttcat atgtggatcc cttagtgtta
     2161 caaattgaac aaacagattt atctggacaa tttgaaggta ttggcgccca tgtgaggaga
     2221 cgagaagacg gcatgataca aattataagc cctatcgaag ggggccctgc agaggccgca
     2281 ggaatcttgg ctggggatat cattttagct gtcgacggag aatcaataga gggactggaa
     2341 ctcttagagg ccgtatcaaa aataaggggc cctaaaggta gtatagtctc cctcacagtg
     2401 aaacatatag gcgaactcga aacaacgatt atagatgtca gcagagacgt tatagcctta
     2461 cccagtgtat tactccggag tgatccgggg gatgacataa cccacatccg gattacagag
     2521 tttaaaggtg acactccagt caggctttcc gaaattttaa ataaggagtt ggaggccggc
     2581 tctaaaggcc taatactcga cttaagaagt aaccctggcg gctatttgca acaggttttt
     2641 gatattgcca acatgttttt agatgacggt gtaatcctcc ttgaggaacg acataccgaa
     2701 aatgtaacat ggcaggcatc gggcggtgga tttgcctcaa ccctaccaat ggtggttctg
     2761 gtaaataagt acagcgccag cgggagtgaa atcctaatgg gggctttcca agataatggg
     2821 agggctacgg taattggaga gcaaaccttt ggtaaaggaa cggtcaatgt cttcagaaaa
     2881 ttaagtaatg gtggcggcct ttatatgtcg gttgggcgtt ggtacactcc taacaaaagg
     2941 ccaatcgaag gggaaggtct cactccagat atattggtca catcacgaga tagcaaggaa
     3001 gcagatataa aacaggttga tgaagcctat aaggaaataa ataaactatt aaatgactaa
     3061 cgagataatc gcatgtcgga taccttaata gcatcaaatc gccgagctta ctatgattat
     3121 gaaatattgg aaactattga ggccggccta gttctccagt cgtcggaaat aaaatcaatc
     3181 cgagatcaca gagtaaattt ggcgggttca tatgcattcc cgtcgaatgg ggaactctgg
     3241 ctgaacaacg cccacattgc ccagtacccc tattccaggg gagaaaacca ccacccactc
     3301 agaagccgga aactgttgtt acgaaagcag gagctcagaa aattcatggc tgcggcacaa
     3361 caaaaaggtt attcgatagt tccattaaag ctttatttgt cagggcattt tgcgaaaatt
     3421 tcaataggga taggtcgcgg caaaagacag tacgacaaga gaaaggcgat tattgagaag
     3481 gaaagatcga gggacgcaca agaggcgatc cgccgtgaat tacaataaga aaagggatgg
     3541 gaactgcgag ttcgcatccc ttttctctgt cccagacagt ttagccgtct agacgagtta
     3601 catgcatggt gtccccagga gcaagggcca ctcttcttac atcgattctg aatttgagta
     3661 accaataagc caatgtggcc ttactgactc caaggtaatc cgctgtggca gtcaaccctt
     3721 gctctgtaac catttcagga agcatttttt ccagtggctg acgaaatctt tcctcaaccg
     3781 cgatcatctt ttttgttttt ctcgttgcca tcaattcctc cgttaatttc gaagactaat
     3841 agatattatt taccaaacca ggcaatgtca acgaagtttt caatcatttc tggctctttt
     3901 ctaatgattt caaaacccgc cgcacctaac gcgccgtgtg cttaacgcta ttaaccttta
     3961 gggacacatt cgcttatacc ttaaggtata gttcgatatc tcttacaatt ctaatttgac
     4021 gtttgccatt caaacttgga aaataattat gtggccctgt agctcagtgg atagagcgac
     4081 tgccttctaa gcagtaagtc gtgggttcga atccctccag ggtcgccacc ctttcattca
     4141 ataaaggtgg gtgggaatgc cccccctacc ccacagaaaa atagcagaac cccacatgac
     4201 aaaccgactt tccgaagaat cgagtcctta tcttttgcaa cataaggaca atcctgtcaa
     4261 ttggtttgcc tggggcctgg acgcctttga agaggctaag gctcgtgatg ttccggtcct
     4321 tttaagcgtc ggctactctt cctgtcactg gtgtcacgtt atggaatacg aatcttttga
     4381 gaatgaagaa atcgcaaaag ctatgaatga tgatttcgta agcgtgaaag tggaccgtga
     4441 agaaaggccc gacatagact ctatctacat gtcggctgtt caatccatga ctggccaggg
     4501 cggctggcct ttgacggttt tcttgacctc tgaagggttg ccgttctttg gaggtacata
     4561 ttacccccca gaaccccgag gaggtatgcc tggtttcatt caaatacttt tggctatatc
     4621 agatgcatac aaaaataggc ggccggagat agaggaatca ggctcgtatc tcctagagca
     4681 tataagagct tcgataacag agactcctgc caccaataat tttgaaccta gtttactgga
     4741 acaggcatac gaacaacttc ttgcacaatg cgattgggaa tggggtgggt tcgggaattc
     4801 gataaaattc cctcagtcca tggccttaga gttcctactc agataccact taaaccaccc
     4861 agattctcga gccctagaaa tggtcgaaac agcccttcaa aaaatggcgt ctggaggtat
     4921 gtacgaccat ttagggggag ggttccacag atattcgact gacaagaaat ggctggtgcc
     4981 tcatttcgaa aaaatgctgt atgacaatgc cttactttcg agactttaca ttcacgcttt
     5041 ccaggttaca ggtaaaaatt tctatcttag ggttgcaaaa gacatatatg aatacattga
     5101 tagggacatg accgatgaaa atggtctgtt ttattccgct gaagatgcgg acagtgaagg
     5161 cgaagaagga accttttacc tatgggaccc taatgaattt aagcgccatt taaccgatct
     5221 agagatggcg ttattcaaat cagcctttag cctaggggaa gaaggtgact tcgaaggcaa
     5281 aaatattctc cactacaaac caaaaggaat ggattttgga tttttgggaa gcgagaaccc
     5341 ggatttactt aatttaagaa agaaattggt tgacataagg aatagtcggc cgaagccatt
     5401 caaagacaat aaaagccttc tatcttggaa tgctctcatg tcactctctt tcatagaagg
     5461 atatcttgta agcaaagagg aaaagttact ggaaatagca aaactaaaca ttaattcatt
     5521 attagacctt attgaattta attccgggct atttcattca tccaagggag gtcaaatagg
     5581 gagttatggc tacctcgagg attattcatc ccttatactg gccatgctgg ctatgcatga
     5641 agcaacatta gacctggaat ggctggctaa aacggaaaaa atagcagaca ctttagtctc
     5701 cttgttctgg aatagtcatg aaacacgact gtacgacact ccagaagacg gtgatgacct
     5761 cattattaga cctagtgaaa gttacgataa cgccacacct tcaggctcat ccatgactgc
     5821 ggaggcatta tttaaattat ctctgataac taacaaatta gaatatttta atatattcat
     5881 atcattaatg agctctactg ccaccatcgc aagtgagcat ccagtctctt tcggtaattg
     5941 gctatgtgtt tacgattctt atatttatcc aac
//
LOCUS       DASCNS010000008         6017 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000074513, whole genome shotgun sequence.
ACCESSION   DASCNS010000008 DASCNS010000000
VERSION     DASCNS010000008.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000074513"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(15..1085)
                     /locus_tag="QF838_00230"
     CDS             complement(15..1085)
                     /locus_tag="QF838_00230"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59154.1"
                     /translation="MTSDKKIRSQQWWQSFQFKDFRYLWVSTFFQSIGFGMDNVALGW
                     IVFEKTDSAFMVGLAAALRMVPLFLLGVFSGLIADNVERRIFLRIFTIVGGGLMALLG
                     VLLIIGNDQIWIIIAIATITGATFAFVLTLRQAYTFDIVGASLSLNGLSMLQIASQTG
                     GVFGALISGILISKLGAGPQFVVIGLMYLISFLVLFGASESGQAASRRRESLNSNFKG
                     YIDLIKEYPVLLILMCLASITEIFGFTHMSLIPVFAKEVLSVGPIGLGILTAIRQVGG
                     FLGLFSLTLLGNYNQKGKLMFYIVVFFGIGQIFLAFSSNVFLFAFFLLVVDACAMSVD
                     TLYKTLMQQNVPKEQRVRAMGS"
     gene            complement(1100..1426)
                     /locus_tag="QF838_00235"
     CDS             complement(1100..1426)
                     /locus_tag="QF838_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019590.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HJO59155.1"
                     /translation="MIYETRIYETHPGKLPNLHDRFRNHTMRIFEKFGIKNIGYWTSE
                     VGEYSDRLTYIIAFENQGHREKAWNDFRNDVEWNKVRTESELEGPIVKRVFNNLLIPT
                     DYSPLQ"
     gene            1622..2554
                     /gene="nudC"
                     /locus_tag="QF838_00240"
     CDS             1622..2554
                     /gene="nudC"
                     /locus_tag="QF838_00240"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HJO59156.1"
                     /translation="MTTHGYGHVFAKNPLNRGEKERRDQSAINSMMTDPQSRFLPLRE
                     LNLLVNTGMESNLFWLSRVQLDEFACKSEPIFLGILDDIYHFAIEVSTDLFSDAVIRS
                     LNPSLRFVDVRSCGEFLDPEEAGIAAQARIQVYWHQRYKFCSACGSKTQMQRAGQVRN
                     CTGCGTDHFPRTDPVIIVVVHDGDKCLLGQSRGRLQRTNTYSALAGFVDQGESIEEAV
                     AREVMEEAGIEVKNVKYHSSQPWPLPYSLMIGCHAEAVTTNINMDEDEMTDVKWFDRK
                     DVISALENNSEVLKVPMKIAIAHHLVKSWALNEI"
     gene            2708..3331
                     /locus_tag="QF838_00245"
     CDS             2708..3331
                     /locus_tag="QF838_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013232.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HJO59157.1"
                     /translation="MLYQDSDLLQFFGGESILYKSHSNSDLVAIEGAISAPIGVSAIQ
                     TAVSRGVKRIFVLGLCGAVDSSLHVGDIVLPTLCEREEGTSFHYLPPEAAASPNYDML
                     QSLTKHLIKYNLPYRAGKTVSTDAPYRQTIETELTWRKDGILGVDMEMSAVFALCEYL
                     TIPAVGLFVVSDAHDLTEKMDWVWNKKLFEENLDKVFTLLLQMTTHF"
     gene            complement(3328..4623)
                     /locus_tag="QF838_00250"
     CDS             complement(3328..4623)
                     /locus_tag="QF838_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59158.1"
                     /translation="MSLPNFNPSSNKFINSFPFFYGWVILFVAALAHFASAPGQTYVS
                     SIFIDPMIDDLGWSRTTFSGLYTGGSMAAAVFMIAVGKMLDKYGARKTLSILCLMMCM
                     ATIWMSGVDSRWKLFLGYAMLRTIGQGSFGLVATTMVSTWVIRIRGRATAISSIGGAA
                     SLAVFPFIVHLLIERFDWRSTWFILGIVVLAVLFIPAITLIRRSPEAVGLKPDGTDHK
                     GTERGDELGKEGSETNFTLIEALKTRSLWMLVFGSTAMPLIMTGLMFHHVSILGAKGI
                     SPGLAALTMGLFGPLVFLGSLGCGYLADRIPNRFLLAAGQLILIVAMLWTIIISAPWQ
                     AIIYIVLASVSMSLMSTPNTVIWANYFGRAYLGSIRGFASTVMVAFSAMGALPFGFIY
                     DRTGSYNDAIFVLLILPVIAGIAYMLALEPKVKPQVNRF"
     gene            4729..5931
                     /locus_tag="QF838_00255"
     CDS             4729..5931
                     /locus_tag="QF838_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO59159.1"
                     /translation="MKITDVKSYLIKMAWDDRPGKDKPRSSIEREFVFVQIDTDEGIT
                     GWGEITNYPGSVGNRAIQKYVMELKDFLIGWDPTKIEEIWTRTFRLFTYTGTRGATTA
                     AISGIDIALWDIFGKSVNLPIYQLLGGSVRDKIPLYTHPPEPGEDIALAIENAREIVE
                     SGHTAFKMDPMMHSSWDGDNGGNIGYLDGEISPEGAYRAEQITAEIRDAVGPDIEILI
                     DAHGKFNVPTAIDLANRLSAYDIHWFEEPVPVESYHALQQVRDSTNVRISVGERLHTR
                     FEFIPVFEKNLADYVMPDVTWTGGISELKKISTMAEAYYIPVSPHDASGPINIMAGAQ
                     VSMTIPNFYKLETIRYDLSGYNCLMDRPLDIRDGNLYLPDEPGLGLNLDEEFLIKHTI
                     PGYGPQNT"
BASE COUNT         1991 a         1281 c         1175 g         1570 t
ORIGIN      
        1 ccaattctta caacctatga tcccatagcc ctaactcgtt gctccttcgg cacattttgc
       61 tgcatcaacg ttttgtacaa agtgtcaaca gacatagcac aggcatcaac aacaagcaga
      121 aaaaatgcaa aaaggaacac gttggacgag aatgccagaa aaatctgacc gataccaaaa
      181 aatacgacga tatagaacat caatttccct ttttgattgt aattgcccaa caaagtaagc
      241 gaaaaaagac ccaaaaaccc accaacttgg cgaatagcag tcaaaattcc caacccaatt
      301 ggtccaacag ataaaacctc cttggcaaaa actgggatta aactcatatg agtgaaacca
      361 aatatttcgg taattgaggc caaacacatc aagataagta aaactggata ttcttttatg
      421 agatcaatgt atcccttaaa attggaatta agactctctc tccttctaga ggctgcttgg
      481 cctgactcag atgctccaaa cagaactaaa aatgaaatca agtacatcaa gcctatgacg
      541 acaaattgtg gtcctgcccc caacttagat atcaatattc cggagatcaa agccccaaaa
      601 acccctcccg tttgagacgc tatttgcaac atagacaaac cattcaatga aagggaagcc
      661 cctacaatat cgaaggtgta tgcctgcctc aaagtaagaa caaatgcaaa tgtggctccg
      721 gtaatagtgg caattgcaat gatgatccat atttggtcgt taccaatgat taggagcacg
      781 ccaagcaagg ccataaggcc cccacctaca atagtaaata tgcgaaggaa tattcgtctt
      841 tcgacattat cagcaatcag gccagaaaaa acaccgagta gaaaaagtgg caccatccta
      901 agagctgctg ctagcccaac catgaatgca gaatcagttt tctcaaaaac aatccagcca
      961 agggctacat tgtccatacc aaacccgata gattggaaaa aagtggacac ccacaaatat
     1021 ctaaaatctt tgaactgaaa ggattgccac cattgttggg atctaatttt tttgtctgag
     1081 gtcaatatct attcacaagc tattgcaaag gagaatagtc ggtcggtatc agcaaattat
     1141 tgaacactct ttttactatt ggtccctcca attctgactc agtccttacc ttgttccact
     1201 caacatcatt cctaaagtca ttccaagctt tctctctatg cccttgattt tcaaaagcaa
     1261 ttatgtaggt caatctatcg ctatattcac caacctctga ggtccaatat ccaatatttt
     1321 ttattccaaa tttttcaaaa atcctcatag tgtggttacg gaatctatca tggaggtttg
     1381 gcaacttacc aggatgggtc tcataaatcc gagtttcata tatcattggt tagtctcctt
     1441 ccatccttag atttcattat tatcccctta ttctgtgttc acaccatcaa gggtgtcaat
     1501 caaaacaact gccccttaga ggacagaata aaaaacttaa gaaagtatcc aacaaaaaat
     1561 agatccacct ttacaacagg aatgtgttct aataacttaa tattcgactt taggagttct
     1621 tatgaccaca catgggtacg gacacgtatt cgcaaagaat ccccttaata ggggagaaaa
     1681 agaaagaaga gaccaatctg caataaacag tatgatgacc gacccacaaa gtcgtttttt
     1741 gcctttaaga gaacttaatt tactggttaa tacaggaatg gaatccaatc tattttggct
     1801 atcacgggtt cagctggatg aattcgcgtg caaatcagag cctatttttt taggcatatt
     1861 ggacgatata tatcacttcg caatagaagt atcgactgat ctcttttcag atgcggtgat
     1921 taggagctta aacccttcac taagattcgt cgacgttagg tcatgtggtg aatttttaga
     1981 tccggaggag gcaggaatcg ccgctcaagc aagaatacaa gtctactggc accaacggta
     2041 caaattctgt tcggcttgcg gatcaaaaac ccaaatgcaa agagctggcc aagtgcgaaa
     2101 ttgtaccgga tgcggtactg atcattttcc aagaaccgat ccagtcataa tagtcgtggt
     2161 acatgatggt gataaatgtt tattaggaca atcaagagga agacttcaaa gaacaaacac
     2221 ctattccgca ttagctggat ttgttgatca aggcgaatca attgaagagg ccgtcgctcg
     2281 agaagtgatg gaagaagctg gaatagaggt taagaatgtt aaataccatt catctcaacc
     2341 ttggccgtta ccttactcct taatgatagg ttgccatgca gaggcagtta ctaccaacat
     2401 caatatggat gaggacgaaa tgactgatgt taaatggttt gataggaaag atgtcatctc
     2461 agcccttgaa aacaacagcg aagtactaaa agtacccatg aaaatagcga ttgctcatca
     2521 tttagtgaaa tcctgggcct taaatgaaat ttgatcttac atgagtagag aaaaatggtt
     2581 taatccaaaa gataaatcac aatgtgtaat aagacccgaa ctacaagata aaacagcatt
     2641 acatttaccc ccaaatctat tggtaatatt ctcgcaaaac tttttgaaaa atttcgttaa
     2701 gagtaatgtg ctttatcaag actcagatct gctccaattc tttggcgggg aatcaatact
     2761 ctataaaagt cacagcaaca gcgatctggt tgccatagaa ggcgccataa gcgctccaat
     2821 aggagtaagc gcgatccaaa cagcggtttc aaggggagtc aaacgaattt ttgtactagg
     2881 cctctgtggt gctgtggata gttctctgca tgtaggggat atagttttac cgacattatg
     2941 tgaaagggaa gagggaacat cttttcacta ccttcctcca gaagcagctg cctcaccgaa
     3001 ctacgatatg ttacaaagtc ttaccaaaca tctcattaaa tataatcttc catatcgtgc
     3061 aggcaaaaca gtaagtaccg atgcaccata tagacaaacg attgaaacag aactcacctg
     3121 gagaaaagac ggaatactgg gggtcgacat ggaaatgtct gccgtctttg cactctgcga
     3181 atacctgaca attccagctg taggtttatt cgtagtttct gacgctcatg acctcacaga
     3241 aaaaatggat tgggtgtgga acaaaaagct atttgaagaa aaccttgata aagtttttac
     3301 tttacttctg cagatgacaa cgcactttta aaaacgattt acttggggct taactttggg
     3361 ttccaaagcc agcatatagg ctattccagc tattacaggc agaatcaata atacaaagat
     3421 cgcatcatta tatgatcctg tcctgtcata tatgaaacca aatggcaaag cacccatcgc
     3481 cgaaaaggcc accataactg tagatgcaaa tcctctgatg cttcccagat aagcccttcc
     3541 aaaataatta gcccaaataa ccgtattagg ggtactcatt aaagacatgc ttactgaagc
     3601 cagaactata taaattattg cttgccatgg ggcagatatt attatggtcc aaagcattgc
     3661 cactatgaga ataagctgac cggcagctag taaaaatcta ttcggaattc tatcggccag
     3721 ataaccgcaa cccaagcttc caagaaaaac taagggtcca aacaatccca ttgtgagtgc
     3781 tgctaagcct ggtgaaattc ctttcgcgcc tagaatagac acatgatgaa acataagccc
     3841 tgtcattatt aacggcattg ccgtgcttcc aaacaccaac atccacaatg atcttgtttt
     3901 gagtgcttca atcaaagtaa agttggtttc tgatccctct tttcctaatt catcaccacg
     3961 ttctgtacct ttatgatcgg tcccatctgg tttcagtcca accgcttctg gactccttct
     4021 gatgagggta atagcaggaa taaaaagaac agccagaacg acaatcccta aaataaacca
     4081 agtactccgc caatcaaagc gttcaattaa caaatgtact ataaaaggga aaactgcaag
     4141 actcgcggct cctccaattg acgatatcgc agttgcacgg cctcttatcc gaataaccca
     4201 agttgaaacc atagtggtgg ctactaatcc aaaggaacct tggccaatag ttctgagcat
     4261 tgcatagccg agaaacaact tccatcgaga atccactcca ctcatccaaa tagtagccat
     4321 acacatcatc aaacataaga tcgacaaagt ttttctcgca ccatatttat ctaacatttt
     4381 cccaacagca atcatgaata ctgctgctgc catggatccc cctgtgtata ggccagaaaa
     4441 agtggtgcgc gaccacccta gatcatcaat catcggatca ataaaaatcg atgagacata
     4501 tgtctgacca ggggccgaag cgaaatgagc caaagcggcg acaaataaaa tgacccatcc
     4561 ataaaaaaaa gggaaagagt ttataaattt gttcgaacta gggttaaaat ttggtaaaga
     4621 cattcatcat cacattaaat attactgaat catgatatac ctttagtaat taagctacga
     4681 tggtaaccgt ggtaaggaat ttattgaagg cagtcagaag gattaaaaat gaaaatcacc
     4741 gatgtgaaaa gctatctcat caaaatggcc tgggatgacc gcccaggaaa agataaaccc
     4801 agatcctcta ttgaacgtga atttgtcttc gtccagattg atacggatga aggaattacc
     4861 ggttggggtg aaattactaa ttatccaggt agtgtcggca atcgagcaat tcaaaaatat
     4921 gttatggaat tgaaggattt tctaatagga tgggatccaa caaagattga agaaatttgg
     4981 actcgcacct ttagactttt cacttatacc ggtactagag gggcaacgac ggcagcaatt
     5041 agtggaatcg atattgcctt gtgggacatc tttggtaaat ccgtgaatct gcctatatat
     5101 cagcttttgg gagggtcagt tagggataaa atacccctct acacccatcc gccagaacct
     5161 ggcgaagaca ttgccctggc aatcgaaaat gctagggaga ttgtggaatc agggcatact
     5221 gccttcaaaa tggatccaat gatgcactct tcatgggatg gcgataatgg cgggaatatc
     5281 gggtatttgg acggagaaat tagtccagaa ggtgcctatc gggccgaaca aattacggca
     5341 gaaattcgag acgcagttgg tccagatatt gagatactta ttgatgctca tggaaaattt
     5401 aatgtcccta cggcaataga cttagccaat cgtctttcgg cctacgacat acattggttt
     5461 gaagaacccg tcccagtcga aagttaccat gccctgcagc aagtaagaga cagtaccaac
     5521 gtaagaataa gcgtaggaga aaggttgcac actagatttg aattcatacc ggtatttgaa
     5581 aaaaacttgg ctgactatgt tatgccagat gttacttgga ctggaggaat atccgaatta
     5641 aagaaaattt ctacaatggc agaggcttat tacataccgg tatctccgca tgacgcgagt
     5701 ggcccaatca acataatggc tggggctcaa gtttctatga caatacctaa tttctataaa
     5761 ttggagacta taaggtacga tctgagtgga tataactgtt taatggacag acctcttgat
     5821 atccgtgatg ggaatttgta tctcccagat gaaccaggtt taggactgaa tttagacgaa
     5881 gagtttctaa tcaaacatac tattccggga tacggtcctc aaaataccta gtctgatggt
     5941 ctcactccca gactattaat agagcccatc aacaccaaat tacctaattc agtattatta
     6001 ttggttgtga gataggg
//
LOCUS       DASCNS010000009         8309 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000095268, whole genome shotgun sequence.
ACCESSION   DASCNS010000009 DASCNS010000000
VERSION     DASCNS010000009.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000095268"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="QF838_00260"
     CDS             <1..147
                     /locus_tag="QF838_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HJO59160.1"
                     /translation="STMYRIHPAYLAWVLDGLLEGVVVNQITVDETTRKYSRIALDRM
                     LSIE"
     gene            227..1072
                     /gene="nadC"
                     /locus_tag="QF838_00265"
     CDS             227..1072
                     /gene="nadC"
                     /locus_tag="QF838_00265"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HJO59161.1"
                     /translation="MQLTPDIEKLIDLAIMEDYSMGDATTDALISNETIGSATVVADE
                     EGTIAGLSLAVRIFQKMDSSLETTVLIPDGSKVTKNDRILEISGYLSSILTAERTALN
                     FLRKLSGVSTETAKYVDEISHTNSRIVDTRKTTPGFRALEKYAVRMGGGHNHRQNLSD
                     GILIKDNHVKTLKLEGLNLTGVIQKAKSNASHTIKVEVEVETVEQAVEAASAGADIIL
                     LDNMSIEEMRKAVESCPDRAVTEASGGINLETIKSIAETGVNLISVGAITHSAPNLDL
                     SLDID"
     gene            complement(1105..1548)
                     /gene="dtd"
                     /locus_tag="QF838_00270"
     CDS             complement(1105..1548)
                     /gene="dtd"
                     /locus_tag="QF838_00270"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HJO59162.1"
                     /translation="MRVILQRVNSANVKVSDAVVGEIQKGLLVLVGIASEDNLEDVDY
                     IVGKTLGLRIFNNQIGKFDFSVQDIEGDLLVVSQFTLYADTRKGKRPSFINAMEPSEA
                     SKLFNITVRKFKESGLKVETGKFQEKMQVSSINDGPVTIFIDSKN"
     gene            1570..1737
                     /locus_tag="QF838_00275"
     CDS             1570..1737
                     /locus_tag="QF838_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59163.1"
                     /translation="MDPDEEELSDEDAVVSFPDLVSVDEDDFESVLDSVVVPSVSEEL
                     MFLPDELPFLP"
     gene            complement(1812..1955)
                     /locus_tag="QF838_00280"
                     /pseudo
     CDS             complement(1812..1955)
                     /locus_tag="QF838_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(2021..4249)
                     /gene="purL"
                     /locus_tag="QF838_00285"
     CDS             complement(2021..4249)
                     /gene="purL"
                     /locus_tag="QF838_00285"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HJO59164.1"
                     /translation="MVLNEDIWIIRNMVFDKEILDELAISPAEYEIIVERLGRVPNHL
                     ELGLFGSLWSEHCGYKHSKPLLKLFASDNPRLLVNPGDENAGVVDIGDGYAIVFKIES
                     HNHPSAIEPYQGAATGIGGIVRDIFAMGARPIALLNSLRFGPLLNDRNKYIFDGVVSG
                     ISGYGNCIGVPNIGGEIVFAECYSGNPLVNAMCIGLLKVDELIKATSGSSGNLLILAG
                     SGTGKDGIHGASGLASRSFEDERELRPTVQVGNPFLEKILIESCLEVAKSGKIDGMQD
                     LGAAGLTSASVECAASGSTGIEIDIDLVPRRDDDMSPYEVMLSETQERMLVSVKPENA
                     ESVKRIFDRWDIDNSIIGKVTTSNRVHIVSGTQLLADLPVGLLTDPPQYVIDSITPPY
                     LRQLQGYDLNRINLPDIAPDMVLMRLLSTSNLTCRQSVYQKYDHQVQTNTVIPPGSDG
                     SLIRIKGTEKGISASTDGNGRLAYLDPYVGGQIAVAEACRNVSCTGAEPIALTNCLNF
                     GNPEKPEVYFQLENCVKGMADASTKFSSPVISGNVSLYNETQNTPIYPTPVVGAVGLT
                     ENVRNYVDMSFKNDDDVALLLGSSEVTCDIGYLAGSEYLEIFHDLIAGQPSIDLEKEI
                     SVQKLCRDLIKHGLVKSAHDCSEGGLAVAIAESCIKGEIGFKGEFEIRGRWDVQLFGE
                     QQSRIVISVKEDDVAEVMRLGIDYQVEVLNLGKVGGERLFVPGLLDVSVADISDAWMV
                     GV"
     gene            complement(4239..4694)
                     /locus_tag="QF838_00290"
     CDS             complement(4239..4694)
                     /locus_tag="QF838_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HJO59165.1"
                     /translation="MNETAKKITYYGFLFAVCAAFLYALSALVGKKITDDFSSPMVAS
                     AFSILFGLVATGSVFFRTVSKEARYLSGRSWVLIGLSGCASALGVSGWYLALNVTQVV
                     VVAPIVAVYPLITILAASLFLRGIEKVTKKTVAGAIIVVIGVLFVGFGT"
     gene            complement(4691..5143)
                     /locus_tag="QF838_00295"
     CDS             complement(4691..5143)
                     /locus_tag="QF838_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HJO59166.1"
                     /translation="MIIYGIKRRLITGILLSLMAALGFGLSAVLVKAGMSSVSVKTAT
                     VISLISSTVVTMIVAFLLHYEEIFSLSPVAFIWFILAGLITFLGGRFLNFHAISLVGA
                     SKASAVVSATPLFAAILAVIFLDENIWVMLGIGTAMIVFGITLVVIQE"
     gene            complement(5118..5816)
                     /gene="purQ"
                     /locus_tag="QF838_00300"
     CDS             complement(5118..5816)
                     /gene="purQ"
                     /locus_tag="QF838_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HJO59167.1"
                     /translation="MKFGVVVFPGTWSDVDCYTVLDEVFNVSVEYVWHKETNLSTFDC
                     IILPGGFSYGDYLRCGAIARFSPVMESLTKFAHSGGLVFGICNGFQILCEAGLLPGVL
                     VRNNHLQFRCQWTDLRVDNTSTPFSSRAKAGQVISVPISHGEGNYFAPDETLRQIENN
                     GQVIFRYSGANGDIDVVNNPNGSINNIAGVTNQEGNVLGMMPHPERCCDSLLGGTDGK
                     LIFGSLVDYLWNKT"
     gene            complement(5822..6268)
                     /locus_tag="QF838_00305"
     CDS             complement(5822..6268)
                     /locus_tag="QF838_00305"
                     /inference="COORDINATES: protein motif:HMM:NF020977.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HJO59168.1"
                     /translation="MLRDTLDSDLKDAMRNKEALKRTVLRTMLSEIRNAEINSQTTLD
                     DEGIISVLTKQVQQRKDSVEAYAAADRQDLVNKETEEINIISVYLPEQLPVEEIEEII
                     KTAISEIGASTLSDMGKVMGLVMPQVRGRADGKIVNTIVTEKLRAL"
     gene            complement(6358..7812)
                     /gene="gltX"
                     /locus_tag="QF838_00310"
     CDS             complement(6358..7812)
                     /gene="gltX"
                     /locus_tag="QF838_00310"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HJO59169.1"
                     /translation="MDFPKIRVRYAPSPTGEPHVGNIRTALFDWLIARNSSGQFIVRV
                     EDTDQARKVEGAIELQKDSLKWLGLDWDEGLGLGGKYGPYTQSERLTLYTDVAERLLQ
                     NGKAYKCFCDGERLSNLRKTQLESKATLVGYDGHCRSLRSDESNSGPFVVRFKMPGSG
                     ISELDDLVRGHLKFDNSLSDDFIILKSDGYPTYHLASVVDDHYMDISHVLRGEEWVSS
                     VPRHVQLYLALEWEMPLFAHLPTILAPDKSKLSKRHGATSVLEYKKAGYLPEAMFNFL
                     SLLGWSIDGDTEIISKEELVSKFDPSRISSASAIFDIEKLNWMNGHYIRSMSSLELSQ
                     ILGNYWREFPPIVFDRTPSPSEIDEVSKLVQERIKVLSEASGLVAFVFKNEITYDSNE
                     LIQKQMDAESTTRILDRVNELVSKIETFSSSAIEVELRALADAMEVKVGTLLGTIRVA
                     TSGQKVSPPLFDSLEVLGRSRVLELLHVAKSKLP"
     gene            complement(7876..>8309)
                     /locus_tag="QF838_00315"
     CDS             complement(7876..>8309)
                     /locus_tag="QF838_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013045.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HJO59170.1"
                     /translation="DKAGSDIGLLFQITDDLIDYTGNTKKAIIDGPIGRDPHDRKKMA
                     IIEKGRDAKTEYAVDERFAQFDCLGVKPKTGRTHQIRVHLCSLGHPVAGDILYGNGVA
                     GLDRQFLHAESLLLAHPSNNSLMEFKSPLPQDLLKFKHSLS"
BASE COUNT         2479 a         1997 c         1491 g         2342 t
ORIGIN      
        1 tctacaatgt atcggattca tccagcctat ctagcctggg tcttggatgg gctactagaa
       61 ggcgtcgtgg taaaccagat aacagtagat gagaccacta gaaaatattc acggatagca
      121 ctggatcgaa tgctatctat agaataaaat acctcccgat tattccatac atactcaaaa
      181 tgccccgccc tcaaacacag gctgggcatt ttgttaggag actaaagtgc aactaacgcc
      241 cgatattgaa aaactcatag acttagcaat catggaagac tactccatgg gtgacgctac
      301 cactgatgca ctgatttcga atgaaactat tggcagtgct acggtagtcg cagacgaaga
      361 aggtaccata gcgggtttaa gccttgctgt gagaatattt caaaaaatgg attcatctct
      421 tgaaaccact gtattgatcc cggacgggtc aaaagtgact aaaaatgacc gtatacttga
      481 aattagtggg tacctatcat caattctgac agctgaaaga actgcactta attttctaag
      541 aaaattgagt ggggtgtcca ctgaaactgc taaatatgtc gatgaaatat ctcatacgaa
      601 ttcccgaatc gtcgatacta gaaaaaccac accagggttc cgagctttgg aaaagtacgc
      661 tgtgagaatg ggtggtggtc ataatcaccg acaaaatctc tctgatggaa tactaataaa
      721 agacaaccat gtaaaaacat taaaactgga gggcttaaac ctgacaggag taatacagaa
      781 agccaaatcc aatgcatctc atactataaa agttgaggtg gaagtcgaaa cggtagaaca
      841 ggctgtagag gctgcatctg ccggagctga cataatattg ttggataata tgtcgattga
      901 agagatgagg aaagccgttg aatcctgccc ggatagagct gtcaccgaag cctccggggg
      961 tattaatttg gaaactatca aatcgatcgc tgaaactggg gtaaatctaa tctcagtagg
     1021 agcgataact cactcggccc cgaacctaga cttgagttta gatattgatt aatagaagga
     1081 ttgcatatta agtcgcatat ctaactagtt ctttgaatca ataaatatag tgacggggcc
     1141 atcattaatc gaagaaactt gcattttctc ttgaaatttt ccagtctcca cctttaatcc
     1201 agattctttg aactttcgaa cggtaatatt gaaaagcttt gatgcctcgc tgggttccat
     1261 agcatttata aaactcggcc ttttaccctt tctggtatcc gcgtataacg taaattggct
     1321 cactactaac aaatcgcctt ctatgtcttg aacagaaaaa tcgaatttac ctatctgatt
     1381 gttgaagatg cgaagcccta gagttttacc tacaatgtag tctacatcct cgaggttatc
     1441 ctcggaggcg attccgacaa gaaccaaaag tcccttttgg atttccccaa caacagcgtc
     1501 cgaaactttt acatttgcag agttaactct ttgcagaatt accctcatta ttccggctga
     1561 gaagtggttt tggatcctga tgaagaagaa ctatccgatg aagacgctgt tgtgtcgttt
     1621 cctgacttag tctctgttga cgaagatgac ttcgagtctg tgctcgattc agtagtagtc
     1681 ccttcagttt cggaggaatt aatgttctta cctgatgagc tacccttttt accgtagtcg
     1741 ttaacatacc aaccgctacc tttaaacact atcggaacta cggaaaattg ccttgtcgcc
     1801 actttgtcac agctgttaca aggttgatcc acagagtcac taaaccgaag cctcagttca
     1861 aattctgagc cacactcttc acatttataa tcatatgttg gcaaacttaa caccgtcccg
     1921 atatcgttat tgcggtcgaa caaataatct agcatcgggt atatgccgtg tcaaacgggc
     1981 ttgcaggtat taccaataaa taaaacaaaa aaacagccct tcaaacccca accatccatg
     2041 catcagaaat atcagcgact gagacgtcca gcaaccccgg cacaaataat cgttcgccac
     2101 caactttacc caaatttagc acttcaacct gataatcaat acctaaacgc atcacctcgg
     2161 caacatcatc ttctttgaca gaaatcacta ttctagattg ttgttcacca aaaagttgaa
     2221 catcccaccg tcctctaatt tcaaattcac ctttgaagcc aatttcacct tttatacaag
     2281 attctgctat tgcgacagcc aatccaccct ctgaacaatc atgagccgat ttgaccaacc
     2341 catgtttaat aagatcccta cataattttt gtaccgagat ttctttctcc aaatctattg
     2401 atggctgtcc agcaattaaa tcgtggaaaa tttcaaggta ttcgcttccg gcaaggtagc
     2461 caatgtcaca ggtaacctcc gaggatccca gcaataacgc tacatcatca tcattcttaa
     2521 agctcatgtc tacataattt ctgacattct cagtgaggcc tacggctcct acgacagggg
     2581 tgggatatat aggagtattt tgtgtttcat tgtataaact cacatttccg cttatcacag
     2641 ggctagaaaa ttttgtacta gcatctgcca tacctttaac acaattctca agttggaaat
     2701 acacctcagg tttttccgga ttaccaaaat tcaaacaatt tgtaagtgct attggttcag
     2761 caccggtaca agacacattc ctgcaagctt cagcgacagc tatttgacca cccacatacg
     2821 ggtccagata cgcaagacgc ccatttccat cagtggaagc cgatatacct ttctccgttc
     2881 cttttatccg gatgagggat ccatcagagc caggtggtat cactgtatta gtttgaacct
     2941 gatgatcata cttttggtaa acggactggc ggcaagtcaa attggaagta gacaaaagcc
     3001 tcattaagac catatccgga gcaatatctg gaagatttat ccgatttaaa tcgtagccct
     3061 gtaattgtcg cagatatgga ggggtaattg aatctatgac atattgcggt gggtctgtaa
     3121 gcaggcctac tggaagatca gccaataatt gagtcccact cactatatga actcgattag
     3181 aagtagtaac tttacctatt atggaattat ctatgtccca tcggtcaaat attcgcttca
     3241 cgctttcagc attctcaggt ttaacggaaa ctaacatacg ctcctgagtc tcagacaaca
     3301 taacctcata tggagacatg tcatcatctc tccgaggtac caaatcaata tcaatctcaa
     3361 taccagtgga tccactagcg gcgcattcca ccgatgcact agtcagaccg gccgccccta
     3421 aatcttgcat tccatcaatt tttccagatt tagcaacttc aagacaagat tcaattagta
     3481 ttttttcgag aaaaggattc ccaacctgga ccgtcggcct caattctctt tcatcttcaa
     3541 agcttctgga agccaagccg gaggctccat gaatcccgtc tttccctgtc ccagaccccg
     3601 ctaggattag taaatttcca ctgcttccag aagtggcttt aatgagctca tctaccttta
     3661 ataaccctat gcacatggca tttactaatg gattgccgga ataacattca gcaaaaacaa
     3721 tttcccctcc tatgttggga acgcctatgc agtttccata cccagaaata cctgatacaa
     3781 ccccgtcaaa aatatattta tttctgtcat ttaacaaagg tccaaacctg agtgagttca
     3841 acagcgcaat aggcctcgca cccatggcaa atatgtcccg cactatacct ccaattccag
     3901 tagcggcccc ctggtatggt tcaatggcag atgggtggtt gtgggactct attttgaaaa
     3961 caattgcata cccatcacct atatcgacta caccggcatt ctcgtctccg ggattcacca
     4021 aaagtctcgg attatctgaa gcaaacaatt taagtagtgg ctttgagtgc ttgtacccgc
     4081 aatgttcgct ccaaagagat ccaaacaatc cgagttctag gtgatttggc acccttccta
     4141 acctttctac gatgatctcg tattcagcgg gggatattgc gagctcgtcc aatatctcct
     4201 tatcaaatac catatttctt ataatccata tatcttcatt aagtaccaaa ccccacaaac
     4261 aatactccaa taactacaat tatcgccccc gcaacagttt tcttggtaac tttttctatc
     4321 cctcgcaaaa atagtgatgc tgccaatatt gttatcaaag gataaacggc tactatggga
     4381 gccacaacca ctacctgagt tacatttagg gccaagtacc accccgaaac acctaaggca
     4441 gaagcacatc cagacaatcc aattagaacc caggatcgac cggacagata tctagcctcc
     4501 ttgcttacag tacgaaaaaa caccgatcct gttgcaacca acccaaacaa aatcgaaaat
     4561 gctgaagcaa ccataggaga ggagaaatca tctgtaattt tcttccccac cagagctgat
     4621 aaagcatata ggaaagccgc acaaacggcg aaaaggaacc catagtacgt tatttttttt
     4681 gcagtctcat tcattcttgt attaccacca aggtaatccc aaaaactatc atagcggtgc
     4741 caatacctaa cattacccaa atattctcat ctaagaaaat tacagccaat atggcggcaa
     4801 ataatggagt cgctgacact actgccgatg cttttgaggc tccaactaag ctaatggcgt
     4861 gaaaattaag aaatcgaccc cccaaaaaag taattaaccc agccaagatg aaccaaataa
     4921 aggccactgg agataaggaa aaaatttctt cataatgcaa taaaaacgct acaatcatgg
     4981 tgacaacagt ggaggaaata agtgatatta ctgtagctgt ttttacactc acagaggaca
     5041 tgcctgcctt tactaaaact gcactcagcc cgaaacctaa ggcggccatc agtgaaagta
     5101 atataccggt aatcaaccta cgttttattc catagataat caaccaggga tccgaatatc
     5161 aatttgccat cagttcctcc cagcagggaa tcacagcaac gttcggggtg aggcatcata
     5221 cctaagacgt ttccttcttg gttagtcact cctgcgatat tatttataga cccgtttggg
     5281 ttattaacga cgtcaatatc accgttcgcg ccactatacc gaaatatcac ctgcccgttg
     5341 ttttcaatct gtctcaaagt ctcatctgga gcaaagtagt ttccttcccc atgcgatatg
     5401 ggaactgaaa ttacttgccc ggccttggct ctactggaaa atggggtgga ggtattatca
     5461 actcttaaat ctgtccattg gcatcgaaat tgcagatggt tgttacgaac caaaaccccc
     5521 gggagtaatc ctgcttcaca caaaatctgg aacccattac atatcccgaa aactaatcct
     5581 ccggaatgag caaatttggt caaagattcc atcaccggag aaaacctcgc tatagccccg
     5641 catcttagat aatcaccgta tgaaaagccc cccggcaata tgatgcagtc aaaggttgaa
     5701 aggttcgtct ccttatgcca aacatattca acggatacgt taaacacctc atctagcacg
     5761 gtgtagcaat ccacgtcgct ccatgtgcct gggaaaacaa caactccaaa tttcacaacg
     5821 tttacaatgc tctcaatttt tcagttacga tcgtattcac aatcttacca tcggcacgac
     5881 ctctaacttg aggcataaca agacccataa ctttccccat atcactcaaa gttgaagccc
     5941 ctatctctga tatggcagtt ttaatgatct cctcgatttc ttctacaggg agctgttccg
     6001 gaagatacac cgatataata ttgatctctt ctgtttcttt attaaccaga tcctgtcgat
     6061 cggcagcagc gtatgcttcc acactatctt ttcgttgctg cacttgctta gtaagaacag
     6121 aaattattcc ctcatcatct aaagttgtct gggaattaat ctcagcgtta cgtatttcag
     6181 atagcattgt tcgtaacact gttcgcttca aagcttcttt gtttctcatc gcgtccttga
     6241 gatctgaatc taaagtatca cgtagcattt ctttgtatat attcctctag gctttggttt
     6301 cgacgtcatg cttctgagta taaattttga ctgccctgct ggtcaatctt aattcgatca
     6361 aggcaatttg gatttggcaa catgcaatag ttccagaact cgactgcgcc ctaacacttc
     6421 caatgagtca aatagaggcg gtgatacctt ctgtccggag gtagcgaccc taatggtacc
     6481 aagcaaggtt ccgactttaa cctccatcgc atcggccagc gcccttaatt caacttcaat
     6541 ggcactagag gaaaaagtct cgatttttga aactaactca ttcactctat ccaatattcg
     6601 ggttgtagat tccgcatcca tctgtttctg tattagctca tttgaatcgt aggtaatttc
     6661 attcttgaaa acaaaggcca ccaaacctga agcttcagaa aggactttga ttctttcttg
     6721 aactaattta gagacctcat ctatctcact cggacttggg gttctatcaa aaactattgg
     6781 agggaattcc ctccaatagt ttccaagtat ttgtgataat tccaagctgc tcattgacct
     6841 aatatagtgt ccattcatcc aatttagctt ttctatatca aaaatggcac ttgcagagct
     6901 tatccggctg ggatcaaatt tactaaccaa ctcttcttta gatattattt cagtgtctcc
     6961 atcaatagac cagccaagta gacttaaaaa attgaacata gcttccggta aatagcctgc
     7021 cttcttatat tccaaaacgg aagttgcccc atggcgctta ctaagtttag atttatcagg
     7081 tgcgagtata gtcggcaaat gagcaaataa cggcatttcc cattccaggg ccaaataaag
     7141 ctggacatgt cgtggcacac ttgacaccca ttcctcaccc ctcaacacat gggatatgtc
     7201 catgtaatgg tcatcaacaa ccgaagctag atggtaagta gggtatccat ctgatttcag
     7261 tattatgaaa tcatcagaaa gggaattatc gaatttcaaa tgacccctca ccagatcatc
     7321 caattctgat atgcccgaac ctggcatctt gaacctcact acgaagggcc cagaattgga
     7381 ttcgtcggat cttagagacc tacaatgccc atcatatcct actagagtcg ctttactttc
     7441 aagttgtgtc tttctcaaat ttgacaacct ctctccatca caaaaacatt tatatgcctt
     7501 cccattttgc aagaggcgct cagccacatc ggtatataaa gttagtcttt ctgactgcgt
     7561 gtaaggacca tacttcccac ccagcccaag tccttcatcc caatccagtc ccaaccattt
     7621 aagagagtct ttttgtaatt ctatagcccc ttccactttc cttgcttgat ctgtatcttc
     7681 aaccctcact ataaattgcc ctgagctgtt tcgagctatc aaccagtcga aaagagccgt
     7741 cctgatattt ccaacatgag gttcgcctgt tggacttgga gcatatctaa ctcttatctt
     7801 gggaaagtcc ataatttaat caaaacctca attattctat caataaccta tttgaatact
     7861 aacactcgct aaattttaac ttagagagtg cttaaattta agcaggtcct gtggtaacgg
     7921 agatttgaat tccataagac tattgttgct agggtgtgct aataaaagac tttctgcatg
     7981 caagaattgc ctgtcaagcc cagcaacccc attgccatat aaaatgtccc ccgctaccgg
     8041 gtgccctaag gaacacaaat gaacccgaat ttgatgagtt cgacctgttt ttggtttcac
     8101 acccaaacaa tcaaattgtg caaatctttc gtctactgca tattctgtct tagcatctcg
     8161 tcctttttcg attattgcca ttttcttcct atcgtgtgga tctcgcccta ttggaccatc
     8221 aattattgct ttttttgtgt tacctgtata atcaataaga tcatcagtta tttgaaataa
     8281 taaaccaata tcagaacctg ctttatcaa
//
LOCUS       DASCNS010000010         1306 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000096567, whole genome shotgun sequence.
ACCESSION   DASCNS010000010 DASCNS010000000
VERSION     DASCNS010000010.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000096567"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="QF838_00320"
     CDS             complement(<1..669)
                     /locus_tag="QF838_00320"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HJO59171.1"
                     /translation="MCPKRLCYTRPKSAHRCQAITTVLPANKDSLEHAANILLEGGVV
                     CIPTDTVYGIAGIATNSIAVSKVFEVKQRDPTTPLPIFIQDISSLDNFSEFVPPLAKI
                     LASNFWPGPLTMVLEKADCIPDVATGGLKTAGFRIPNHEIAVKLCEKTGNPITGTSAN
                     ISGQKAATTADEALRQFKDSSLDLIIDGGESTSSDPSTIVHFENNEVKLIRQGVISMS
                     DIQKL"
     gene            672..>1306
                     /gene="guaA"
                     /locus_tag="QF838_00325"
     CDS             672..>1306
                     /gene="guaA"
                     /locus_tag="QF838_00325"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HJO59172.1"
                     /translation="MNEEQGLDSTRRITSSDDLEVSAYLEISQEKVGNGPATEGEMSP
                     QRESILIVDFGSQYSRLIARRVRESSVYCEIISFNTSWSEVEGLNPKGIILSGGPASV
                     YEETSPLAPLWVFESGLPILGICYGMQVLVHQLGGKVAASTKKEYGHAVLHQNKPNEP
                     LFTGLPQSLPVWMSHADRVTELPPGFVSMAHTDNSPIAVVGNDKGILGIQF"
BASE COUNT          358 a          252 c          305 g          391 t
ORIGIN      
        1 cagtttttgt atgtcagaca tagatataac accttgtctt atcagtttaa cctcattgtt
       61 ttcaaaatgg actatagtgg aaggatctga cgaggtagat tcgccaccat cgataatcag
      121 atctagagag gaatctttaa attggcgtag agcctcatct gctgtggtgg ctgccttttg
      181 gccactgatg tttgcactgg tcccagtgat gggatttcct gttttttcac atagttttac
      241 tgctatctca tggttaggta tacggaaacc agctgttttt aatcctcctg tggccacatc
      301 gggtatgcaa tctgcttttt ccaataccat agtcaacggt ccaggccaga aattagaagc
      361 taaaattttg gctagaggag ggacaaattc cgagaaattg tccagagaag agatatcttg
      421 gatgaaaatt gggagtggtg tggtagggtc tctttgtttc acttcaaaca cttttgagac
      481 tgctatggaa tttgtagcta tacctgcaat tccatatacg gtatcagtgg gtatgcagac
      541 aacaccacct tccaatagta tattagcagc atgttccaaa gaatctttat ttgcgggtaa
      601 tacggtggtt atggcttggc acctgtgagc tgattttggg cgagtataac ataggcgttt
      661 aggacacaat tatgaacgaa gagcaaggat tagatagtac tcgtcgcata acttctagcg
      721 acgaccttga agtttcagcc taccttgaaa tatctcaaga aaaagtaggg aatggaccag
      781 ccactgaagg cgagatgtca ccccagagag aatctatttt aattgtagat tttggatccc
      841 aatacagccg cttaattgct agaagggtca gggaaagtag tgtttattgt gaaatcattt
      901 cctttaacac tagctggagt gaagtcgagg gtttgaaccc aaaagggatc atactctcag
      961 gtggacctgc tagtgtttat gaggagactt ctccgctagc ccctctatgg gtttttgagt
     1021 cagggttacc tattttggga atttgctatg ggatgcaggt tttggttcat caattgggtg
     1081 gtaaagtggc ggcatcaacc aagaaagaat atggacatgc cgtactacat caaaacaaac
     1141 caaatgaacc tctttttact gggctacctc aatcattacc tgtctggatg agtcatgccg
     1201 atcgggttac tgaattacct cccggatttg tatctatggc ccataccgac aattctccaa
     1261 ttgctgtagt tggtaacgac aaaggcattc tagggataca gtttca
//
LOCUS       DASCNS010000011         4062 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000102352, whole genome shotgun sequence.
ACCESSION   DASCNS010000011 DASCNS010000000
VERSION     DASCNS010000011.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000102352"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /gene="argS"
                     /locus_tag="QF838_00330"
     CDS             complement(<1..397)
                     /gene="argS"
                     /locus_tag="QF838_00330"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013565.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HJO59173.1"
                     /translation="MLELYDTLTRKKSALRPHEKGKFSVYTCGPTVYRDAHIGNLRSY
                     LMADWIRRTLEILGHEVTHIKNITDVGHMRQEVLEQGDDKVIAAAIAEGKTPAQIAEF
                     YTQRFLEDESTINILPANEFPKATEHVNEM"
     gene            complement(443..1897)
                     /gene="pyk"
                     /locus_tag="QF838_00335"
     CDS             complement(443..1897)
                     /gene="pyk"
                     /locus_tag="QF838_00335"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HJO59174.1"
                     /translation="MSYTNPVDLYQGKPKTKIVCTLGPSSDSEEVLIELIKTGMNVAR
                     LNMSHGDKNVHEIVFNRVRKVSKELGIPVGIMVDVPGAKYRTGPTDPGALELSEKDKI
                     TLTSRDLIGSKTLVSVAPPGIHLDAEINGTILVDDGNLEFVVEDISGENVNCTVVRGG
                     RLTERRGVVTPGRAPSQKFPDEKAIECLKFAAKFGADFVALSTVTRREDIWTARSILR
                     ENGMNKPLIISKVERAEAIDNLDEVVDASDALMVARGDMGVEIPLHRVPVVQKELIRK
                     SNSAGKPVITATQMLESMVHSSSPTRAEVTDVANAVFDGSDAIMLSGETSIGKFPIEA
                     VKIMVNTSKEAEAALPYHKMMEERFLQLESKTDDVISYAACQTSNLLGSKFIIAFTES
                     GNSAGRVSRYRPESRILALTPWEEVCRVLTLRWGVYPVIVDTPTDVDEFFSVGEEEAR
                     KFMEGDNKGTVVLVAGTPIGVPGSTNMLRVLQLG"
     gene            2080..2790
                     /locus_tag="QF838_00340"
     CDS             2080..2790
                     /locus_tag="QF838_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59175.1"
                     /translation="MYDVSLLLDLQTLDRSISNLRKDLSDTEASLTDDSAVVKAGSIF
                     QQISTAHQTVNKNQREIEREVQRLTDRKDSIESKIYAGEISNPKELTALQGEAANIGD
                     LIEAKEEVLLTSMEETERYNQGLAKATAQLETAEMSHQEKVSGLKSKQQQLINQLHAE
                     EPAQQAARELCSAHLLHIYDRFGKANKGIAIALVESDRCSECRLSIPKKLLEMLKTAS
                     EFVYCNSCGRILYRAVYK"
     gene            2787..>4062
                     /locus_tag="QF838_00345"
     CDS             2787..>4062
                     /locus_tag="QF838_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019149.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HJO59176.1"
                     /translation="MRVIGYCNNLVDSFQDQNDFALSVDNFSDQFNHQISFVIQDEVI
                     PIGSSPNFKSLLDLVEKDSAGYLIIIPDARHIGADLESVARNVISVEKANSSFYCLNN
                     DYPDVLQNALVHINAPGVSVSKSNNIKDGMQNRAMSGKSLGKPPFGYLTNESGEFTIT
                     EPEAVVVRQIFEMYVNQDMGLRKIVQFLNDSNIRTRRGKPWNIVTVRDILKNSSYIGT
                     YQRFGLRLTRNHDPIINPELFRAAQDKVRERRKYKGFPTLEPYLLSGLCQCGYCGNTM
                     MGVTRKQNWRRQGGERIKNTYRYYQCQSKGNQGTCGYHTWTTDKLEARVLKMLTTMAN
                     NGKLEESVSGQRGEAKFRLAAENRFAKVVSAEKRFVEFMKKTARGESVVGRLELYLKA
                     LDNAREQAFISCQPKEISTLIETWNEQPFTRRQ"
BASE COUNT         1189 a          912 c          848 g         1113 t
ORIGIN      
        1 tcatctcgtt tacgtgttca gtcgcctttg gaaattcatt ggcaggtaat atatttatgg
       61 tcgattcgtc ttcgaggaaa cgttgggtat aaaactctgc tatttgggcc ggagtcttac
      121 cctctgctat ggcggctgcg atcactttat catcgccctg ctcgagtacc tcctgcctca
      181 tatgtccaac gtcggttatg ttctttatat gggtcacctc atggccaaga atctccaatg
      241 tccgccgaat ccagtcagcc atcaagtaag atcgcaagtt cccgatatgg gcatcacggt
      301 ataccgttgg cccacatgta tagacactga atttaccttt ttcgtgaggt cttaaggcag
      361 atttttttct tgtgagagtg tcatatagct ctaacactcg cacctccttg tttgtgcagt
      421 aacttagaat ttaatgaacc gactatccca attgtagaac tcttagcata tttgttgaac
      481 ctggcacgcc aataggtgtc ccggcaacta gcacgactgt ccccttatta tccccttcca
      541 taaatttcct agcttcttct tctcccacgg aaaagaattc gtctacgtcc gtaggagtat
      601 ctacaatgac tggatatact ccccatctca gggtcaagac tctgcacact tcttcccaag
      661 gggttaaagc taatatacgc gactctggtc ggtatcgtga cacacgtcct gcagaatttc
      721 ctgactcagt aaatgcaatt atgaattttg aaccaagtaa gttcgaggtc tggcaggccg
      781 cgtagctgat aacatcatcg gtctttgact ccaattggag aaaccgttct tccatcattt
      841 tatggtaggg taatgctgcc tctgcctcct tggacgtatt caccatgatt ttcacggctt
      901 ctatggggaa ttttcctatc gacgtttccc ctgacagcat gatcgcgtct gatccatcaa
      961 agacagcatt ggcaacatca gttacctctg cccgagtcgg ggaagaagaa tgaaccatag
     1021 actcaagcat ttgagttgct gttataacag gtttaccagc agaattactt ttccggatca
     1081 actctttttg gactactgga actcggtgca atggtatttc cacacccatg tcccctctag
     1141 cgaccataag agcatcgctg gcatcgacga cctcatctag attatctatg gcttcagctc
     1201 tctcgacctt tgatattatc aacggtttgt tcataccgtt ctcacggaga attgatctgg
     1261 cagtccagat atcttctctg cgcgttacag tggacaaagc cacaaaatcg gcgccgaatt
     1321 tagctgcaaa tttaaggcat tcgattgctt tttcatcggg aaatttttga gatggagccc
     1381 ggccgggggt aaccacccct cgtctttctg tcagccttcc gccgcggacg acagtacaat
     1441 ttacattctc acccgatatg tcctcgacaa caaactccag gttcccatca tcgaccaaaa
     1501 tagtgccgtt aatttcagcg tctaaatgga tgccgggggg agcaacacta actagggtct
     1561 tactaccaat tagatctcta ctagttaaag tgatcttatc tttttctgac agttccaatg
     1621 ccccaggatc tgtagggccg gtacggtatt tagcacccgg aacatccacc attataccca
     1681 caggtatacc caactccttg gatacttttc taaccctgtt aaaaactatc tcatggacat
     1741 ttttatcccc gtgggacatg ttgaggcggg ccacattcat accagtttta atcaactcta
     1801 ttaaaacttc ctcactatca gaggaggggc cgagagtaca aactattttt gtcttaggtt
     1861 taccttgata aagatccacg ggattcgtat aggacatata aaccttttta attgatctta
     1921 aaccgacatt tttcgggcaa gtttacataa ctatacatta ctactccaac cggtcctcac
     1981 tgttctggtg ggtttaagtt agagtatgga ccccatattt tagatttgcc tgaaacactg
     2041 cgttgaatta aagttcgtct aaaggattcc tggtgaaata tgtatgacgt atctttactc
     2101 ttagacctac aaaccttaga caggtccatt tccaatttac gaaaagacct atcagatact
     2161 gaggcctctc ttaccgacga tagtgctgta gttaaggctg gtagcatatt ccagcagatt
     2221 tcgactgccc atcaaactgt caataaaaat cagagggaaa ttgaacgaga ggtacagcgt
     2281 ttaaccgatc gaaaggattc catcgaatcc aaaatttacg ccggtgaaat aagcaaccct
     2341 aaagagctaa ctgccctaca gggcgaagca gctaatatag gtgatctaat tgaggccaaa
     2401 gaagaagtgt tgctaaccag tatggaagaa accgaaagat acaatcaggg tttagcaaag
     2461 gcaacggcac agttggaaac cgctgaaatg agtcatcagg aaaaagtatc gggtctcaaa
     2521 tcaaaacaac aacaactgat caatcaactc catgcagagg agccagccca acaagctgcc
     2581 cgagagttat gttcagcgca ccttcttcat atttacgaca ggttcggtaa agcaaacaaa
     2641 ggtatagcga tcgccttggt ggaaagcgat aggtgctctg aatgccgtct atctattccc
     2701 aaaaaacttc tggaaatgct taagacggcc agtgaattcg tgtattgcaa ttcttgcgga
     2761 agaatactct atagagccgt atataaatga gggttatcgg atattgcaat aatttagttg
     2821 attcctttca agaccaaaat gactttgctt tatctgttga taatttctcg gatcaattca
     2881 accatcaaat aagttttgtc atccaggacg aggtcattcc tattggatct agtccaaact
     2941 tcaaaagcct cctcgatctt gttgagaaag actctgctgg gtatctaatc ataattcctg
     3001 atgctcgtca tataggagct gatttagaat ctgtagctag aaatgtcatc tctgtagaaa
     3061 aagctaattc ttcattttat tgtttaaata atgattatcc agacgtgtta caaaatgcac
     3121 ttgtacatat aaatgcgccg ggggtttctg tatccaaaag taataacatc aaagatggga
     3181 tgcaaaatag agcgatgtcg ggcaaatcgt taggaaaacc tccctttggt tacctcacca
     3241 atgagtcagg ggaatttaca atcactgaac cagaagcggt ggtggttagg caaatttttg
     3301 aaatgtatgt gaaccaagat atgggtctcc gaaaaatagt ccaattctta aacgactcaa
     3361 atattcgcac gagaagagga aagccatgga atatagtcac cgttcgagat atattgaaga
     3421 attcttcata cataggcacc tatcaacgct tcggactaag acttactaga aaccacgatc
     3481 ccattataaa tccggaatta ttcagagcgg ctcaagacaa agttagagaa agaagaaaat
     3541 ataaaggatt ccctacgctt gaaccttacc tgctttccgg cctgtgtcag tgcggatatt
     3601 gcggaaacac aatgatggga gtcactagaa agcaaaactg gagaaggcaa ggcggcgaga
     3661 ggatcaaaaa tacttatcgg tattaccaat gtcagtcaaa ggggaatcag ggaacctgtg
     3721 gctaccacac ttggaccact gataaattgg aagccagggt cctcaagatg ttaaccacca
     3781 tggcaaataa cgggaaacta gaagaaagtg tctcaggcca aagaggtgag gcaaaattcc
     3841 gattagctgc tgaaaacaga tttgccaaag ttgtaagtgc agagaaaaga ttcgtggaat
     3901 ttatgaagaa gacggctaga ggtgaatctg tagttggccg attggagcta tatctcaaag
     3961 ccttggacaa tgcacgggaa caagctttca ttagctgtca acccaaagaa atctcgacct
     4021 taatcgagac ctggaatgaa caacctttta ccagacgaca gg
//
LOCUS       DASCNS010000012         7752 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000108511, whole genome shotgun sequence.
ACCESSION   DASCNS010000012 DASCNS010000000
VERSION     DASCNS010000012.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000108511"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="QF838_00350"
     CDS             complement(<1..652)
                     /locus_tag="QF838_00350"
                     /inference="COORDINATES: protein motif:HMM:NF020039.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HJO59177.1"
                     /translation="MPILDVKKDVSYCVGSFGKGLDHPECVTWGSDGYAYAGGEGGQL
                     YRINVTENKYEQFSQASHFVGGMCQDGSGNLYFCSGNKVYKTNSKGEISEYSSGTSAV
                     PMVGPNYPAFDTDGNLYVTDSGEWEKDNGRIFKISPSGQTKIWSEALQTFPNGICLSS
                     DGSHLYVAVSLNPPRIERIPIDAEGEPGNPEIVVDMPNSVPDGLAFDSDGNLYISCY"
     gene            1073..1360
                     /locus_tag="QF838_00355"
     CDS             1073..1360
                     /locus_tag="QF838_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59178.1"
                     /translation="MHLLEVSNPVAQQRGMINILKPNPRPSSLDGKTIGLIWSGTHGG
                     DLALNRAGEMIQERFSNVVVNFYTGGNYPTPPAIVEQAAKECDLVIGATAD"
     gene            1391..2710
                     /locus_tag="QF838_00360"
     CDS             1391..2710
                     /locus_tag="QF838_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59179.1"
                     /translation="MIEVEKMGKPAVPIVSGRFEDDALASSRTFGMPDLQFVIVPRIY
                     RNLADDLCVSQTEDAIDDLIASLTSDETQAAQSESDESILRFEGEDRYDAVLKMNQEF
                     TGRDWSDALPIWPPTREAVEKLIEGTNLNADDVVCDMPPGFGIATVEQIAINSAMAGA
                     KPEHMPIIIAAVKCLSVTGGHGGKSMLMSTSPQAPMLVVNGPIARKIGLNGGSALGPG
                     RENEINITIGRAFYLCLKNIGMWYPHKMDMDTIGTTRKFVPCLAENEAASPWASYAAD
                     RGFSDDQSAVSIFTTNGELDVQDQGNTSAEGLLKTLAYGITFGNRSVGHLQSQERLIF
                     MPPDVALPVAAEGWSKEDAKNFIFEHAYGSLGKFIQFMPVEDARVEEEWQWIRDKTEQ
                     ELLETNIPLLADPDKIDIVVLGADRAKTLVFPTGPSAETVGIDQYLP"
     gene            2862..4073
                     /locus_tag="QF838_00365"
     CDS             2862..4073
                     /locus_tag="QF838_00365"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59180.1"
                     /translation="MSTEKNNGGPLSGIRMLDLGRYQAGPRCALMFARMGAEVIKVEA
                     LKGDESRQNGPTVRGQSAYWVQYNSGKKSLAIDLRTDKGKEVLTRLVKKSDVFLQNFR
                     PGTIDIMGFGYDVLKKLNPKIIMINASAYGQDGPHRDKVGFDPIGQAMGGMMSLTGLK
                     GDPPTKTYFPLIDRITSLHATVGALAALWEREISGEGQAIDVSLADTGFTVNEIPLTA
                     YLGDGYITERSTSYGGADNPLGGMYETSDGFVIIAAGNNNIWKRMCEAIDKPEWLTDD
                     RFISRASRAENGAAINDVLSIWFSERKMKDVVDYLSGFSIPCAPVNSVAQAAQESHLH
                     ERGILMEVPDPIAGKIHVAGKMIKFSRTEMVVGSTPTVGQHTEEILRDIAEYTEDEIK
                     ELDDNNVVARS"
     gene            4175..5155
                     /locus_tag="QF838_00370"
     CDS             4175..5155
                     /locus_tag="QF838_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HJO59181.1"
                     /translation="MSHDEINRESIMWMYQKMLTIRRFEEQARREADAGKLRGIHSSI
                     GQEAVPTGVCAHLRDEDFVLGTHRSHHHCIAKGVDLNEMMAELLGKSTGTGKGKGGTM
                     HIADINKGMLGANGIVGSNIPVATGVALTAKVKGTDNVSVVFFGDGASSQGALHESMN
                     LASIWALPVIFVCENNRYAESTPFEYSVAGGSVSNRADGYAIPGVTVDGQSVLDVFEV
                     AKEAVGRARAGEGPTLIEAQTYRYLGHAGHDDPLTYRSEEEQKYYMDRDCITNFKSYI
                     LDSSMANEEELDNIEVACEVAVAKSVEFADNSPFPDPGALTEDVYTFYKN"
     gene            complement(5183..6613)
                     /locus_tag="QF838_00375"
     CDS             complement(5183..6613)
                     /locus_tag="QF838_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO59182.1"
                     /translation="MSKKIKIEAKIIVAYQAGEHRILRDGCVVVEGDHISFVGKTFDQ
                     PVDEVIDAGDRIITPGFINTHCHMAGSPLDKSFIEDVGSRQFYLSGLPVMLRARGGAM
                     RDEDKEACVDYSIVELIRTGTTTIMEQGGMGDYVADAAEQAGLRAYIANMYSSASWIT
                     PSGRQVEYDWNEAAGIEGFKKAVDFVERIDGRANGRIKGFLGPAQIDNCTEELLRMSS
                     EASQEMQVPVSLHTSQAVFEFQEITRRYEKTPVEWLDSIDFLNDWALLGHVIYPAGHS
                     WVNFAGDDLSILERTKASVAHATWVFNRRGIAMESFPGYLDAGVNVCLGTDTCPQSMI
                     EAMRWSAVVGKIISRQTEKSTAADVFNAATLNPAQMLHRDDLGRISAGAKADLLFWDT
                     SSLFMVPLRDPIKNIVYNATADELKDVMIDGEWVMKGREVLKINEQQANEKLQIAGEH
                     VWSSVNPEVASSADELSPQTFSSFKV"
     gene            6970..>7752
                     /locus_tag="QF838_00380"
     CDS             6970..>7752
                     /locus_tag="QF838_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HJO59183.1"
                     /translation="MIKGKQDQVLQQLNSFSPKLIGQLKNLTEEDWSAQSRCDDWTVK
                     DVMAHLSSVVTNYLIIIDSAKNEENILDDPNSSRDEPGSLDGKSLAGRLSERAKEASK
                     NFDSKDALINELELRFQRLLDGFNSCQDNQWNLPAYHPVNWVSIQGILLWTLFESTIH
                     AWDALNAVNEKYLVDKGLAPLLPEYFCNKLINRWFKTADSTETPSQTLAVNFSTSNGL
                     RIASALGELDIEELPINSFDSDAEIHTSPSEFALLITGRKSLS"
BASE COUNT         2319 a         1682 c         1712 g         2039 t
ORIGIN      
        1 tataacatga aatataaagg ttcccgtctg agtcaaacgc caacccatca ggaacagaat
       61 ttggcatatc aacaacaatt tccggattac ccggttcgcc ctctgcgtca atgggtattc
      121 gttcaatacg aggcggattc aaactgacgg cgacatataa atgcgaccca tcggaagaaa
      181 ggcaaatccc atttggaaag gtttgtaacg cttctgacca aatttttgtt tgaccactag
      241 gactgatttt aaatatccgt ccgttatctt tttcccactc gccagagtcc gttacataaa
      301 ggtttccatc cgtatcgaaa gcaggataat taggccccac cattggcaca gccgaggttc
      361 ccgaagagta ctcacttatc tccccttttg aattggtttt ataaactttg ttgccactac
      421 aaaaatatag gttccccgaa ccgtcttggc acatgcctcc cacaaaatgc gaggcctggg
      481 aaaactgctc gtatttattt tctgtaacat ttatacgata taactgacct ccttctccac
      541 ctgcgtatgc atatccatca ctaccccaag tgacacattc agggtgatca agaccttttc
      601 caaaacttcc aacacaatag gatacatcct ttttaacatc tagtatcggc atctctattt
      661 cacctatttt tcaaatattg caaatgtatt ttcaatccta cgttaattac taattcattc
      721 taaagattca atttggtttt acaaatagac tgctgcctta aacaaaaatt attgaatagt
      781 gtgtccgatt ggattttcag acctgattgc aacataaatt tctagggtat caaaggagca
      841 aggaagggat ttagtaggtg aagcccctga aataccaaaa tgcttttcat tctttgtcgt
      901 accaaaaatt cttctccgat ttattcgtca tattttctgt gtcggcatca tggctcccag
      961 caaaaataca cctggttcaa agaagaagga gtatttgaag tcgtttcaat atagtggtaa
     1021 aaatagctaa tgaaattaaa agtttcatat atttaataat taggagttag atatgcatct
     1081 tttggaagtc tctaatcctg tcgctcagca gcgaggtatg atcaatatac tcaaaccgaa
     1141 tcctcggccg agttctctgg acggcaagac gatcggtcta atatggagtg gcactcacgg
     1201 aggagacctt gctctcaacc gggctggtga aatgatacag gaacgcttta gcaacgttgt
     1261 cgttaatttc tacacaggcg gtaactaccc aacccctcct gctatcgtag agcaggcagc
     1321 gaaggaatgt gacctggtaa taggtgccac tgctgattga ggaacctgcg cctcgtggat
     1381 gtgccacgac atgatagaag tcgaaaagat ggggaaaccc gcagtcccga tagtttcggg
     1441 acgttttgag gacgacgcct tggctagtag tagaacattt ggaatgcctg acctgcaatt
     1501 cgtaatagtt cctaggattt atagaaactt ggctgacgac ctttgtgtat cccaaacgga
     1561 agatgctata gacgatttga tcgccagtct gacatcggat gaaactcagg ctgcacaatc
     1621 ggaaagcgat gaatcgattt taagattcga gggagaagat cggtacgatg cagtcctgaa
     1681 gatgaatcaa gaatttacag gccgtgattg gtccgacgct ctgcctattt ggcctcctac
     1741 aagagaggcc gttgaaaagc taatagaggg cactaatttg aatgctgatg atgtggtgtg
     1801 cgacatgccc cccgggttcg ggattgccac agtggaacaa atcgcgatca actcggctat
     1861 ggcaggagca aaacccgaac acatgcccat cattatcgca gctgttaaat gtctatcggt
     1921 aaccggtggt catggtggca aatctatgct gatgtcaacg agcccacagg ccccaatgtt
     1981 ggttgtgaat ggtcctatcg cgaggaaaat aggcctcaat ggaggatcag cattaggacc
     2041 cggtagagaa aatgaaataa atattacaat aggcagggct ttttaccttt gcttaaaaaa
     2101 tataggtatg tggtacccgc ataagatgga catggacacc ataggtacaa ccagaaagtt
     2161 tgtgccatgt ttagccgaaa atgaagccgc cagcccgtgg gcttcatatg cagctgacag
     2221 aggtttctca gatgaccaga gtgccgtttc tattttcacc accaacggag aactagacgt
     2281 gcaagaccaa ggtaatacat ctgcagaagg attacttaag actttggcat atggaatcac
     2341 tttcggtaat cgctcggttg gccatttaca gagccaggaa cgacttatat tcatgccgcc
     2401 tgatgtcgca ttaccggttg ccgctgaagg ttggtcaaaa gaggatgcaa agaattttat
     2461 ttttgagcac gcttacggaa gcctcggtaa attcatacaa ttcatgcctg ttgaagatgc
     2521 tagggtagaa gaagaatggc agtggatacg tgacaaaact gaacaagaat tactggaaac
     2581 caatataccg ctattagccg acccagacaa aatagatatt gtcgtgcttg gtgctgacag
     2641 ggcaaaaaca cttgttttcc cgacgggacc aagtgcagag actgtcggaa tcgatcagta
     2701 tctaccgtaa aatttagcct tcgtgaatga caacgaacac acaattaatc aaaattatca
     2761 accaggtttg tgcttaacgg ttctgtccgt tgcgcaaggg aacgtgcggc cacagattat
     2821 ttctaacaat caacggattg aaaagtaagg taaggtgaaa aatgtcaacc gaaaagaata
     2881 atggtggacc actttccggc atcagaatgc tggatctagg acgctatcaa gcaggtccaa
     2941 gatgtgccct catgttcgcc cgtatgggtg cagaggttat taaagtcgaa gccctcaaag
     3001 gtgatgaaag caggcaaaat ggtccgacag taagaggcca gagtgcctat tgggtacagt
     3061 acaacagtgg taagaaaagc ctagccatag atctccgaac agacaaggga aaagaggttt
     3121 tgacgagatt agtcaaaaag tctgatgtct tccttcagaa ttttcggcct ggaaccatcg
     3181 atattatggg atttgggtat gatgttctca aaaaacttaa cccgaaaatt atcatgatta
     3241 acgcttccgc ctacggccaa gacggtccac accgggataa agttggcttt gaccctatcg
     3301 ggcaagcaat gggcggcatg atgtccctaa cagggctcaa aggggaccct ccaactaaga
     3361 cttacttccc tctaatagat cgaataacct ctctccatgc aaccgtcggg gccttggctg
     3421 ccctgtggga aagggaaatc tcaggagaag gccaagcaat tgacgtatca ctggctgata
     3481 cagggttcac cgtcaatgaa ataccactaa ccgcctatct tggcgatggc tatataactg
     3541 aaagatctac tagctatggt ggtgcagaca acccgcttgg aggaatgtac gagacttcag
     3601 atggatttgt gattattgca gccggaaata acaacatatg gaaaaggatg tgcgaggcga
     3661 tagataagcc agaatggtta acggacgacc ggtttatttc ccgggcgtct agagctgaga
     3721 atggcgcggc aataaatgat gtgctatcta tttggttttc agaaaggaaa atgaaggatg
     3781 tggtggacta cctatctggt tttagtattc cttgcgctcc ggtaaatagt gtggctcagg
     3841 cagcacagga atcccatcta catgaaagag ggattcttat ggaggtccct gacccaatcg
     3901 ctgggaagat acacgttgcc ggtaaaatga ttaagtttag tcggacagaa atggtagtgg
     3961 gatcaacacc tactgtcgga caacataccg aagaaatatt aagggatata gcggaataca
     4021 cggaagatga gattaaagag ttagatgaca ataacgtggt tgctcgaagc taacttattc
     4081 gaaatttaca ctatgataac cttcgtagaa ggcatgaata gatcgtgaga ttactcaagg
     4141 ttacaactaa aaaatttgaa ctgaggtacg tccaatgagt catgatgaga ttaacagaga
     4201 atccataatg tggatgtacc agaagatgct cactattcga cgttttgaag aacaggcaag
     4261 acgtgaagca gatgcgggaa aattaagagg aatacattct tctattggac aggaagcagt
     4321 cccaacagga gtgtgtgctc acctcagaga tgaagacttt gtccttggca ctcacagaag
     4381 ccaccatcac tgcattgcca aaggagtcga tctaaatgaa atgatggcag agttattggg
     4441 taaatctaca ggaaccggga aaggaaaggg cggcacgatg catattgccg acataaataa
     4501 aggaatgctg ggagctaatg gaatagtggg ttccaacatt ccagtggcca caggagtagc
     4561 tttaaccgcc aaagttaagg ggactgacaa tgtgagtgtg gttttcttcg gtgatggtgc
     4621 ttcaagccaa ggggccctcc atgaatcgat gaatttggca agcatatggg ctcttccagt
     4681 catattcgtt tgcgagaaca acaggtatgc cgagtcaact cctttcgaat attccgtcgc
     4741 gggaggatcc gtttctaata gagccgatgg atatgcaatt ccaggtgtaa cagtagatgg
     4801 acaatcagtg ctagatgttt ttgaagttgc aaaagaagct gtaggaagag caagggccgg
     4861 agaagggcct actcttatag aagctcaaac ttaccgatac ctaggacatg caggtcatga
     4921 cgatccgctt acttataggt cagaagaaga gcaaaagtac tacatggatc gggactgtat
     4981 taccaatttt aaaagctaca tattagacag ctctatggcc aacgaagagg aattagataa
     5041 tatcgaagtt gcttgtgaag ttgcggtcgc taaatctgtc gaatttgctg acaacagtcc
     5101 tttccccgac cctggtgctc ttacagagga tgtttataca ttctataaaa actagttaat
     5161 cctggttctg caacagacag aattaaacct taaagctgga gaaagtttga ggagataatt
     5221 cgtccgcgct gctggcgact tctggattaa ctgaagacca cacgtgctct cccgcaattt
     5281 gaagtttctc attggcttgc tgttcattaa ttttcaaaac ttcccttcct ttcataaccc
     5341 attcaccgtc gatcatgaca tcttttaatt cgtccgcagt cgcgttatat acgatgtttt
     5401 taatgggatc gcgtaaggga accataaaca gagatgacgt atcccagaaa agtaggtcag
     5461 ccttagctcc agctgagata cgccctaaat catctcggtg tagcatctga gccgggttga
     5521 gagtagctgc gttaaagacg tctgcagcag tagatttttc agtctgacga cttataattt
     5581 tgcccactac agcagaccaa cgcatcgctt caatcataga ttgagggcat gtatctgtac
     5641 caaggcatac atttactcca gcgtctagat accctgggaa tgattccatc gctatacctc
     5701 gcctgttaaa aacccaggta gcatgggcaa ccgaagcttt cgtccgttcc aaaattgata
     5761 agtcgtctcc tgcaaaattt acccaactat gacctgccgg gtaaatgacg tgtcctaaaa
     5821 gagcccagtc attgagaaaa tcaattgaat ctaaccattc cacgggagtc ttctcgtatc
     5881 ttcgggtaat ttcctgaaat tcaaaaactg cttggctagt atgtaaactt acggggacct
     5941 gcatttcttg ggaggcctca ctactcatac gaagcaactc ttcggtgcag ttatcaattt
     6001 gagcaggacc taaaaatccc tttattctcc cattagcacg tccatctatc cgttctacaa
     6061 aatcgactgc ctttttaaaa ccttcaattc cggctgcttc attccaatcg tattcaacct
     6121 gcctaccgct tggggtaatc caactagcag agctatacat gttagctata taggcccgta
     6181 gtccagcctg ctccgcagcg tcagccacat aatctcccat tccaccttgc tccatgatgg
     6241 ttgtggttcc ggttcttatg agttctacaa tggaataatc cacgcatgcc tctttgtcct
     6301 cgtccctcat tgccccacct cgagctctta acatcactgg caagcctgac aaataaaatt
     6361 gcctgcttcc tacatcttca ataaaggatt tatccaaagg tgaccccgcc atatggcaat
     6421 gggtatttat aaacccagga gttattatcc gatcccctgc atctatgact tcatcgacag
     6481 gttgatcaaa agtcttccct acaaaactta tgtggtcgcc ttcaacgaca acacagccat
     6541 cccgaaggat acgatgttct ccggcctgat aggcaacaat aattttggcc tcaattttta
     6601 tctttttaga caactctcac ctctgcagta tttactaagc tttatagagc atatcggctt
     6661 aactgccgac aaggaccttt atttgcaact gatgcattat ctcttcttta ttcggaaata
     6721 ctatctttac tacgatcagg taatcaatat ccctcctata agaacgaagg ttaagctaaa
     6781 tctcgatgag tttgcagagc ttactaatgg tgccttccga tcaataagca caatagttgc
     6841 cagaaaaatc ccataaatcc cctacaccgt tacaatacca atattaggat tatgaaaaat
     6901 caggataggt ggcgccgcta aggtgctacc tccatcattc aagaagcttc tctgagaaag
     6961 ggggtaggtt tgataaaagg aaaacaggat caggttttgc aacaattaaa tagcttctct
     7021 ccaaaattga tcggccaact aaaaaactta actgaagaag attggtcagc tcaaagtcgc
     7081 tgcgatgact ggacagtgaa agatgtaatg gcacacctat catcagtggt gactaattat
     7141 cttattatta tagattcggc aaaaaatgaa gaaaacattt tagatgatcc caatagttcg
     7201 agagatgaac caggttctct agatggcaaa tcactagctg gacgcctctc agagagagct
     7261 aaggaagcta gtaaaaattt cgattcaaaa gatgcactaa tcaacgaatt agaacttaga
     7321 tttcagagat tattggatgg tttcaattct tgccaagaca accaatggaa tttgccagca
     7381 tatcaccccg taaattgggt ttcgattcag ggaatccttt tatggactct gtttgagtcg
     7441 acaattcatg cctgggatgc cttaaatgct gtaaatgaaa aatatttggt cgacaaagga
     7501 ttagcaccac tactgcctga gtatttctgc aataagctta tcaaccgatg gtttaaaacc
     7561 gctgattcta ctgagacccc ttcacagacg ctagcagtta attttagtac ttcaaatggg
     7621 ctacggatag ccagcgccct aggtgaattg gatatagaag agctcccaat taattccttt
     7681 gattcagatg cggaaataca cacctcgcca agtgaattcg cattgctaat tacaggccgg
     7741 aaaagcctat ca
//
LOCUS       DASCNS010000013        19415 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000122279, whole genome shotgun sequence.
ACCESSION   DASCNS010000013 DASCNS010000000
VERSION     DASCNS010000013.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 19415)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19415)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19415
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000122279"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(111..929)
                     /locus_tag="QF838_00385"
     CDS             complement(111..929)
                     /locus_tag="QF838_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59184.1"
                     /translation="MVTDPGDRVVESYVETDNSVETRKAVSDGLLGVHNVTSNRFWQF
                     AALFGIMFIFGIVGFVMRLSSGFDDKGAWGYYVAVFAFLMTTSSAAPMVAIAPRIANS
                     HWRRPISRAAEIWTLAGCLNLILYIPLIWLLPSLENGRRSLWFYGQQTAQDMDSCWVF
                     CWGGGVPAYSPHIWATLAIVGLVLLGLSLLWLSTLPDFAVLRDHGEGWKKKWAGRLAL
                     GWRGTSAQWNWQHHRMGVVGAFYFMMLVFVHFFISVEFLMVHVPGWIDSLAASR"
     gene            complement(919..1659)
                     /locus_tag="QF838_00390"
     CDS             complement(919..1659)
                     /locus_tag="QF838_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HJO59185.1"
                     /translation="MVDSNKWGMVVDIDRCTGCQGCVVACQSENNIPINLEEHFNQRR
                     AIQWIRIERYWEGEYPDVKARFIPIMCQHCGNAPCEPVCPVYATYHNDEGMNVQVYNR
                     CIGTRFCANGCPYHVRFFNFWEPEWPESLKNQLNPDVTVRSRGIMEKCSFCVQRIQRS
                     TRESERDNEVLEDGDRGLNPACVNACASNSLIFGNFNDPDSTVSKMKEDAMQEGERGY
                     QLMENLGTDTNVIYLKKVDESAEVTHGY"
     gene            complement(1698..3716)
                     /locus_tag="QF838_00395"
     CDS             complement(1698..3716)
                     /locus_tag="QF838_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJO59186.1"
                     /translation="MEGNPDHPLNLGGHSALSEAALQGLYHPDRISAPQVRTGARGSG
                     EYREISWEDAIARLSSSLGELDSSNENNKAVFVSNPTGGHVGLVLEKFTDSLGTRHLS
                     YEALETNVLRTALKAVFGTDSIPEFDIDNADLVLSFGADFLSSWVSPTRYARGYGEFR
                     QGHEHRGKLIHVDSRFSMTAANADQWVHVNPGTEGLLAMSIMQVLSSSHPNAVFGDLD
                     VDGVLDSYSPANVANEIGVSVDTINQIAEDFSHSEHPIALGGGSAGAHTNGTQNLKAI
                     YSLNHVASNLGKKGGVLLNPNSPFDGVGAAPPSNSFSEFHRLAEEMKAGNVKVLMVKD
                     ADFYYGMTDTVDMKAALDSVPLVVSFGGMMDDTTSIADLILPQHHFFEDWGTDVPLAG
                     PGYQMIGFQQPVVRPFFEHRGSNLGTKGFADILMATAQVMGKDLGLDGETFKEVIRND
                     AKRLYELDRGQIVSNSFEGFWNGVLQRGGWWDTSYKVDEVPRVENLAGVASPEFGGLD
                     QEFYIQPFISTLGDGSAASLPWLQAMPDAISTATWQTWVEINHKVAEEKGISEGDVLE
                     IRPSNGRVVKALAMPNPAVPPDTIGIPIGQGHRDGGRYSSGRGANILSILDPKLDSEN
                     GSLAWAATRANVTTTGEWIRVPKFENTVPEFPRDEHHHIIQITSGDNH"
     gene            complement(3688..3945)
                     /locus_tag="QF838_00400"
     CDS             complement(3688..3945)
                     /locus_tag="QF838_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59187.1"
                     /translation="MALTRRQFLTLMGGSAGAAVLFQACGLPEKELLIDSPPAMPEDL
                     VSGIDNWYATTNQQGGSSEGIVVRVMEGRAKKSGRKSRPST"
     gene            complement(3955..4557)
                     /locus_tag="QF838_00405"
     CDS             complement(3955..4557)
                     /locus_tag="QF838_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025875.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HJO59188.1"
                     /translation="MIPGDFQPQKPTGTQLAKLGALGAVLLGGFIGIVLVLTFVISSW
                     LGRPVIFGHDGPDQPIEFPHETHVKELGMDCTFCHRNVEKEAAASVPATGLCMTCHSA
                     VGDELEGITKMRSLYEDGRSIHWVRVHRVPDHVHFVHEAHIRYFSEKDGIEAKDVCAT
                     CHGDVASMDKVQQNRALKMGDCVNCHKQNNAPTDCATCHY"
     gene            complement(4575..4874)
                     /locus_tag="QF838_00410"
     CDS             complement(4575..4874)
                     /locus_tag="QF838_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59189.1"
                     /translation="MEIDPNYINSAVWADIKIFTHNMKFFVLFIIIFAGNMLIGHNAI
                     PSLVKSRHLPASLSKLRPPIYLFAVVSFVAAIYFVVTAFAGGLDAIRAIYPDFWI"
     gene            5170..5925
                     /locus_tag="QF838_00415"
     CDS             5170..5925
                     /locus_tag="QF838_00415"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009504370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO59190.1"
                     /translation="MERLKNKVALITGGSRGMGAAEAKMFAGEGAHVVIADILENEGR
                     KTASEIAASGARCVFTHLDVLKASQWDSAIGTAISIFGKLDVLVNNAGVTSRMMLLDT
                     SEPEWDRVMDINTKGTFLGIKAAIPAIRYSGGGSIINISSQMGLVGADYISPQYQASK
                     GAVRLLTKSVAIQYASDNIRCNSIHPAPIETEMTADVRQDPDAFQDMLRRIPMGRYGK
                     PEEVAYAALYLASDESSFVTGSELVVDGGWTAQ"
     gene            5968..7119
                     /locus_tag="QF838_00420"
     CDS             5968..7119
                     /locus_tag="QF838_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO59191.1"
                     /translation="MKITDISHYIIHPGVGKNLCFVKIDTDEGVEGWGECYTQADRDK
                     QVVSHIDQLKRYLIGRNPMHIKHFLQMSFDDFAGRRGAMDFYCAISGLEQAMWDISGK
                     ICQVPVYMLLGGPCRDKIRVYANGWGGGNWDSDEIAEKAKKIADMGFTAMKFDPIPGP
                     WRTFVDKEVERQAVENVKAVRSAVGPDIDILVEMHRRLAPMHAVKIAKEIEEFNPFWF
                     EEPVLAENVDALASAKKVINIPVVTGEELYTKFEFREIFEKQAADIINPDVCNVGGIL
                     ELKEIAAMAEPYYVAVSPHNYNSTTLGLSATIQVSAAIPNFLITEYFVNFENLGKDIS
                     THPFEVDNGYIKLPTEPGLGMDLIEENLEKYPYKQFPPRNLAQYSDEGP"
     gene            7294..8226
                     /locus_tag="QF838_00425"
     CDS             7294..8226
                     /locus_tag="QF838_00425"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HJO59192.1"
                     /translation="MGLSQELNIKKDLRDSFITIGVFDGVHLGHRHLAKKLIESAMKN
                     GKSSGIITFKNHPAAVINPRFQPNFLTSFETKLDLLKNTGVDFVSAITFDHKVADLTA
                     EDFLSLLKNRLGMKGLVVGPDFQMGKNREADTDRLATLGSKLGFELEVVDVQTKGGDQ
                     VRSTMIRSLISDGDVKNASVMLGRKFSLEGSVITGMKRGKSLGFPTANLKTNEGIAIP
                     SNGIYATVATIKGVRYPAATSVGTNPTFPGKEQTIETYILDFDKNIYGQSLSISFYEK
                     LRNELTFDSVDNLIEQMKSDVSEVRKLNKSGELQ"
     gene            8306..9502
                     /gene="tyrS"
                     /locus_tag="QF838_00430"
     CDS             8306..9502
                     /gene="tyrS"
                     /locus_tag="QF838_00430"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HJO59193.1"
                     /translation="MPLTEKEDILSRGVEEIIVKSEFIDRLNSGQKMRLKMGFDPSAP
                     DIHVGHAVGLRKLRQLQEMGHQVVLIVGDWTAQIGDPSGRSQTRKIISIEEVHSNAQT
                     YLDQFFKIVDKERTEIRWQSEWFGPFTLTNVIELTSKFTVAQILAREDFAKRFGENQP
                     ISITELLYPLLQGYDSVAIESDVEFGGTDQKFNLLVGRELQQMRGMKAQQCFIMPLLP
                     GTDGVQKMSKSLGNYIGIDETPQEIFGKVMSIPDSLILPYFDWLTDTPTKEITEIKNS
                     LETQSVNPIELKKNVAYKLVTDFHDAKSASSSRDFFEKTVQGGQAPDDMPEYVIPSGN
                     AAAGLKLSNILVASGLTSSSGEARRLIEQGAVKINEIQITQNQPVNRLEKGILRAGRR
                     RYVRLI"
     gene            9614..10708
                     /locus_tag="QF838_00435"
     CDS             9614..10708
                     /locus_tag="QF838_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HJO59194.1"
                     /translation="MVNLRIPGPIPVNDDILESMGTPMINHRGPEFKEILYRTTERLK
                     TVFSTKNDVYIITGSGTSAMEAAVVNTLSPGDKAINATVGVFGNRFGVISERYGIDLV
                     SLEFPFGSDVDLDVLRNCLNANPDVKAVMVTHNETSTGVTNNLKGISDVVKKEYDKLI
                     LVDGISSVCSLPLETDLWDLDVVATASQKGWMLPPGLAFISFSEKAWDAHSRSTMPRF
                     YLDMAEYKRYYEIGQPPFTPSVSCMFALDLALDQIITEGMGSVFERHASIGQYTRDCI
                     AELNLNIFPQSNKIASNTCTAVEVPEGVDGSKLVSKMRDEHKVVLAGGQATLSGKIFR
                     IGHMGQTTESDIKEAIDALKIVLPEIGFKN"
     gene            complement(10723..12555)
                     /gene="ftsH"
                     /locus_tag="QF838_00440"
     CDS             complement(10723..12555)
                     /gene="ftsH"
                     /locus_tag="QF838_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HJO59195.1"
                     /translation="MNMRSMRSMFIYLLIAVGIMAIFLTVFKEPFGGSSEIPISRVIS
                     LASENKLESIEISGDSLTAVGSDGSQYTSRKESGSSIVEQLEVAGVRSGAGGVDVSVR
                     GAGGIGSFFGILINFLPLIFFGAILLFMMKQAQGSTSQTFSFGKSKARVFDSDNPTVS
                     FSDVAGVDEAKEELQEVVEFLKSPERFLALGAKIPRGVLLMGPPGVGKTLLARAVAGE
                     AAVPFFSISGSEFVEMFVGVGASRVRDLFDQAKKHAPCIVFVDEIDAVGRHRGAGLGG
                     GHDEREQTLNQILVEMDGFDTGTNVIVLASTNRPDILDPALLRPGRFDRRVTLDSPDV
                     KGREEILNVHSKGKPISEDTDMEAIAKQTAGFSGADLMNLLNESAILAARRDKKTITY
                     DEMAESIDRVIAGPERKNKVVSEREKSMVAHHEAGHALVAHLLPHADPPFKVTIVARG
                     QTGGHTRFIPEEETQLVTQNQLEARLASALGGRVAEELVFFEVTTGAGNDLEQATNIA
                     RAMITRLGMSKKLGPRTFGKREELVFLGREISEQKDYSDTVAQTIDEEVHGLIQHAYE
                     IARNLIDTNMGKLTALASHLIEHETIEGDDLKKLLDEASAVASN"
     gene            12822..13505
                     /locus_tag="QF838_00445"
     CDS             12822..13505
                     /locus_tag="QF838_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HJO59196.1"
                     /translation="MNIAHNKSNGLTRENICQLLAEEMVTPIFVSNSNESETFMGQVD
                     IAEASPLIVMDLGFLNRAQTSKISELCKEKNLPILAIISSEDLMSLDTDMIITDFIVA
                     PFPEEELIVRSIFSVKRATTGPLKEELINRGDLTINPSNYEVLINNSRINLRFKEYEL
                     LLLLASNPGRVYDRATLLNQIWGYDYFGGTRTVDVHIRRLRSKIENNPENPYIETIWN
                     VGYRFRSSS"
     gene            13634..14614
                     /locus_tag="QF838_00450"
     CDS             13634..14614
                     /locus_tag="QF838_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HJO59197.1"
                     /translation="MNTNLKINDALLLQMYKEMVLCRTLDERIWMLNRQGKAAIMASS
                     QGHEAGQIGSVAALRKGYDQCYIYYRDLAVLLTLGLTPAEIIKGFIAKKGEPLSGARQ
                     FPTHGAHPTYGVINLSNVIATHIPQAVGAALTAKIMKEDRVIVAYFGDGASSAGDCHE
                     AMNFAAIHKLPVIFFCENNGYAISVPLSKQMAVDNVAARAEGYGMPGIEIDGCDIAAV
                     YETTIEAVARARAGLGPTLIEANVERYLPHTSDDDDSIYRSSQEIEEAKKRDPLKLLR
                     EQLLERKIMNLKEDTYIHEDAKDIVNKATEEAENAPYPETVDFFKHVYAE"
     gene            14624..15607
                     /locus_tag="QF838_00455"
     CDS             14624..15607
                     /locus_tag="QF838_00455"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HJO59198.1"
                     /translation="MAVITVLEAIREAMREEMRNDERVFIMGEDIGARGGVFLATDGF
                     LSEFGEERVIDTPLAESSIAGIALGAAMSGLRPIAEIEFADFIWPTINQIVGEAAKVR
                     YGTEGKLNAPLVLRAPQGGGVRGALYHSQSVEALFAHTPGLKVVTPSTPYDAKGLLKS
                     AIRDDDPVIFLEHKRTYRLVKGEVPEQEYTIPIGKADVKKPGEDISIITYGLMVHHCL
                     EAAYLASSENISVEVLDLRTVRPLDIESIIGTVEKTGKALIVYEDNQALGIGSEVAAL
                     ISQHAFEYLDAPVIRLGGPEVPAMPFSPPLEDMFMIDPGKISTAIKELAEY"
     gene            15613..16902
                     /locus_tag="QF838_00460"
     CDS             15613..16902
                     /locus_tag="QF838_00460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HJO59199.1"
                     /translation="MKIELPQVGESVTEGIIGRWLKNVGDKVEKYDPLVEVITDKVNM
                     ELPSPVRGVLRKIIANEGETVLMGGFIADIETSENQEGANDYKPEPDTTGQEIGTTGV
                     LLKNTAPVGPTGSGGPIHQDESKSTPDKKQLSKGRYSPAVLRLAEENKIDLSQIAGTG
                     INGRITRKDVAAFIESDLTPPQNKELPPMPANDTNPRKDQEKISLSPVRKMIAANMVK
                     SATLIPQAWSEVAVDVTGMVLKRQSIKKSFMDKEGINITYLPFVVKAVAESLKKNPLL
                     NSSWSEEGIILKKNINISIAVAAPGGLVVPVIHNADLFSIAGIAGIIENLVTKAKNGK
                     LDLPEVQGGTFTLNNTGALGSVSSRPLVNHPQAAIMTTEAIVKHPVVREDAIAIRSIM
                     NICLSFDHRIMDGAEASAFSMTVKNLLETINPETPIY"
     gene            16915..17595
                     /gene="lipB"
                     /locus_tag="QF838_00465"
     CDS             16915..17595
                     /gene="lipB"
                     /locus_tag="QF838_00465"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HJO59200.1"
                     /translation="MLTTYYGLVRYDIGQAIQQKTHAKVAAGGDDQVLIFQHPNVYTL
                     GRMGRLEDILIETDAIRRLNLEIRNTDRGGEVTYHGPGQLVVYPIIKLDRIELTPSAY
                     VNSLKVSIAECLADYGIITNEKNMPTGVWVEDRKIAAIGVRISKKTTSHGLALNVTTD
                     LSYFDHIIPCGIHNCVITSIKEEIGDHLKIEDVAHKLACKLGKALGNRITWKDDSPNQ
                     DSDPSPAI"
     gene            complement(17561..18208)
                     /gene="phoU"
                     /locus_tag="QF838_00470"
     CDS             complement(17561..18208)
                     /gene="phoU"
                     /locus_tag="QF838_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HJO59201.1"
                     /translation="MPREDFENNLKVVQDELIQLSSMVEKAVFKSIDALRNRDINASQ
                     QVIDDDDAIDEKQQDIEDKCIELIALEAPMAGDLRVIISVMMMATELERIGDYAEGIG
                     KISLAMGDLPPLKPLIDIPRMSDLATDMLRRSTEAFINRDTALATSVRDDDDVVDELY
                     HQVYRELLTFMMADPSTIQRATYLIWVAHDLERVADRTTNMSERVIWLVTGQNPD"
     gene            complement(18220..18990)
                     /gene="pstB"
                     /locus_tag="QF838_00475"
     CDS             complement(18220..18990)
                     /gene="pstB"
                     /locus_tag="QF838_00475"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HJO59202.1"
                     /translation="MADSTVISVKGLNVYYEDFQAVKDVDMEIKRESVTALIGPSGCG
                     KSTYLRCLNRMNDLIMGASIQGEVLFENRNIYDSNVDATEIRSRIGMVFQKPNPFPKS
                     IYQNIAFGLKVNGFSGDMEEAVEKSLKQAALWDEVKDRLNDSAFTLSGGQQQRLCIAR
                     ALAVEPEVILMDEPASALDPIATLAIEDLVRELKTDYTIVIVTHNMQQAARVSDETAF
                     FMVGEDRSGYLVEQDDTQKIFTNPTHQRTEEYITGRFS"
     gene            complement(18992..>19415)
                     /locus_tag="QF838_00480"
     CDS             complement(18992..>19415)
                     /locus_tag="QF838_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJO59203.1"
                     /translation="LLVLPIVILASRESIRAVPDSYRQGAYAMGADKWQVIKGAVLPS
                     AFSGILTGTILAMSRAIGEAAPVIAITALVYITYIPAHPFERFTVLPIQIFNWVARPQ
                     DDFRSLAAAGIIVLLVILLTMNAIAVFLRNKYQTRSEE"
BASE COUNT         5997 a         4370 c         3946 g         5102 t
ORIGIN      
        1 cgccacactc gcgactttgg tcagacccat gtaggagatc aacacgacgc cgaccatgga
       61 tacaacaaca tcgccaccct tatgaaagaa cccggagaag tggccggggt ctatctggac
      121 gccgccaaag aatctatcca acctggaacg tgaaccatca aaaactctac actgataaaa
      181 aagtgtacga acacaagcat catgaaataa aaagccccca caacccccat tcgatgatgc
      241 tgccaattcc attgagctga agtgcctctc caacctagtg ctagccttcc agcccacttc
      301 tttttccaac cttccccatg gtcccttaaa accgcgaaat ccggtaaagt cgataaccac
      361 aacaaagaca gccctaataa aaccaatccc actattgcca gcgtggccca tatatgagga
      421 gaataagccg gaacacctcc cccccaacaa aatacccaac aactatccat gtcttgagct
      481 gtttgctgac catagaacca aaggcttctt ctaccatttt ccagggacgg aagtagccaa
      541 attaagggta tgtacagaat taggttcaga cacccggcaa gagtccatat ttcggcagct
      601 cttgatatcg gcctcctcca atgcgaatta gcaatacgag gtgctatggc gaccattggt
      661 gcagccgagg aagtagtcat caagaaggca aatactgcta cgtagtatcc ccaggccccc
      721 ttatcgtcaa atcccgagct caacctcata acaaacccga caatcccgaa tataaacatg
      781 attccaaaca aggccgcaaa ttgccagaat ctattcgagg taacgttatg aactcccaga
      841 aggccatctg atacggcctt tctggtttcc acagaattat ctgtctctac ataagattct
      901 actaccctat caccaggatc agtaaccatg cgtgacctca gcagattcat caactttttt
      961 aaggtatata acgttagtat ctgtacctaa attctccatc aattgatatc cacgttcccc
     1021 ttcctgcatt gcgtcctctt tcattttgga caccgtacta tcagggtcgt taaaattacc
     1081 gaaaataagc gaattactgg cacaggcatt cacgcaggcc ggattaagac ctctgtctcc
     1141 atcctctaaa acctcattat ctctttcaga ttctctggtc gacctttgaa ttctttgaac
     1201 gcaaaaactg catttctcca ttattccgcg acttctaacc gtcacatccg gatttagctg
     1261 attcttgaga ctttcaggcc attctggctc ccagaaattg aaaaacctga catgataagg
     1321 gcatccattc gcacaaaatc ttgttcctat gcagcgatta taaacctgta cgttcattcc
     1381 ctcatcattg tgataggtag cgtagactgg acaaacgggc tcgcaaggtg cgtttccgca
     1441 gtgctgacac ataataggaa taaaccgagc cttcacgtct gggtattcac cttcccaata
     1501 cctttcaatc cgtatccatt gtatagctct gcgctgatta aaatgttctt ctaaattgat
     1561 gggtatattg ttctcagatt gacaggccac aacgcaccct tggcacccag tacaccgatc
     1621 aatatcaaca accatccccc atttattaga atctaccatc gatttgctcc tattgtttcc
     1681 ttaaaggttc taatatccta atgattatca ccagaagtga tttgaataat atggtgatgt
     1741 tcgtcgcgtg gaaattcggg aacagtattt tcaaatttcg gaacgcgaat ccattcaccg
     1801 gttgtagtta cattggctct agttgcagcc catgccaatg acccattttc agagtccagt
     1861 ttaggatcta gaatcgacaa aatatttgca cctctgcctg atgagtacct gcccccatcc
     1921 ctgtggcctt gtccaatagg aatccctata gtgtctggtg gcacagcagg gttaggcatt
     1981 gccaaggctt taactactcg gccatttgat ggtcggatct ccagcacatc cccttccgat
     2041 attccctttt cttcagcaac tttatgattt atttcaaccc aagtttgcca ggtggcagtc
     2101 gatatagcgt ctggcatagc ctgaagccat ggcaaagaag ctgcactacc atcacctaaa
     2161 gtagaaataa atggttgtat ataaaattct tgatcaagcc ccccaaactc tggagaagct
     2221 acaccagcaa gattctcaac tctggggacc tcatctacct tataggatgt gtcccaccaa
     2281 ccgcctcttt gaagaactcc attccaaaag ccctcaaagc tattcgaaac tatctggcct
     2341 cgatccaatt catataacct tttggcatcg tttctaatta cttctttaaa tgtttctcca
     2401 tccaatccta ggtctttacc cattacctgg gcagtagcca taagtatatc ggcgaacccc
     2461 ttggttccaa ggttactacc tctgtgctca aagaaaggtc ttactacggg ctgctgaaat
     2521 cctatcatct gatagccggg gccagcgagt ggaacatccg taccccaatc ttcaaaaaag
     2581 tggtgttgag gcaatattaa gtcagctatg gaagtagtat cgtccatcat acctccaaaa
     2641 ctaacaacca aaggcacgct atccaaggct gctttcatgt cgacggtatc cgtcatccca
     2701 taataaaagt ccgcatcttt aaccatcaaa accttaacat ttccggcttt catttcttca
     2761 gccagccgat gaaattctga aaatgaatta gaagggggcg ccgcaccaac tccatcgaaa
     2821 ggtgagttcg ggttcaaaag aaccccacct ttttttccta ggtttgaagc cacatggttt
     2881 agggaataaa tagcttttag attttgggta ccgttagtat gcgcaccggc agaacctcca
     2941 cccagagcta tcgggtgttc actatgagag aaatcctcgg cgatttgatt gatagtatcg
     3001 acagatactc caatttcatt cgctacattg gcaggtgagt aagaatccag caccccatca
     3061 acatccagat ctccaaaaac cgcattcgga tggctcgaag ataaaacttg catgatactc
     3121 atcgcaagca acccttcagt gccaggatta acatgcaccc attggtcagc atttgcagca
     3181 gtcatggaaa accttgaatc tacatgtatg agcttacccc tgtgctcatg tccctgccta
     3241 aattctccat atcccctggc gtatctcgtg ggagacaccc aagaagacaa gaaatctgct
     3301 ccaaaagaca ggaccaaatc tgcattatca atgtcgaatt caggaatgga atcggtgcca
     3361 aacactgctt taagagcggt tctgagaaca ttcgtctcca atgcctcata cgacaaatga
     3421 cgagtaccga gcgaatcagt gaatttttcc agcacaagcc caacatggcc gccagttggg
     3481 ttactaacaa aaaccgcctt attattttcg ttagaactat caagttcacc caaactcgag
     3541 ctcagtcttg ctatcgcatc ttcccagctt atttctctat actcaccaga acctcttgca
     3601 ccagttctaa cttgtggagc acttatacgg tcaggatgat agagtccttg aagggccgct
     3661 tcagaaaggg cactatgccc accgaggtta agtggatggt ctggatttcc ttccactttt
     3721 ttttgctcta ccctccatta ctcttacaac gatcccctct gagctaccgc cctgctgatt
     3781 tgtagttgcg taccagttat cgattccgga caccaaatcc tcaggcatag caggaggaga
     3841 gtcgattaaa agttctttct caggtaaccc acaagcctgg aacagcacag cagcgccggc
     3901 tgacccaccc atgagagtca aaaattgtcg tctagttaaa gccatggact acctctaata
     3961 gtgacatgta gcacagtccg ttggtgcatt gttttgcttg tgacagttaa cacagtctcc
     4021 cattttgaga gctctatttt gctgaacttt atccatactt gccacatctc cgtgacaagt
     4081 ggcacacaca tctttagctt ctatcccatc tttctcggaa aagtatctta tgtgggcttc
     4141 atgtacaaaa tgaacatgat caggaactct atggactcta acccaatgga tagatcttcc
     4201 atcctcgtaa aggcttctca tttttgttat accttctaat tcgtccccaa cagccgagtg
     4261 gcatgtcatg caaagacctg tggccggaac agatgcagca gcttcttttt cgacattccg
     4321 gtggcaaaag gtgcaatcca taccgagctc tttcacatgg gtttcatgcg gaaattcgat
     4381 tggctggtct ggaccatcgt ggccaaatat aactggccta cccagccatg atgaaatcac
     4441 aaaagttaaa accagaacta tacctatgaa ccccccgagc aagacagcac caagagcccc
     4501 cagcttagca agctgggttc ccgtcggttt ctgaggctgg aaatcgcctg gtatcaattc
     4561 ttcactgtcc tatgttaaat ccaaaaatca gggtatatag ctctaatagc atctaatccg
     4621 ccagcaaatg ctgtgacaac aaagtaaatt gcagcaacga aagacacaac ggcaaacaaa
     4681 tatatagggg gccggagctt tgaaagcgag gctggcagat gacgagattt aaccaaagag
     4741 ggtatcgcgt tgtgacctat cagcatattg ccagcaaaaa tgattataaa caacacaaaa
     4801 aatttcatgt tgtgcgtgaa aatttttata tccgcccaga cggcggagtt aatataattt
     4861 ggatctatct ccaattgcct gtttccacct tttttttccg acgagcatag cctaattatc
     4921 gcctagtcaa tcgggtgaaa attatcacat tccaatattg ggggtgtcaa gggaactctc
     4981 agccaaaaat cgccaaaaat ctgccgagtg agtaaaacaa tgttaataga acctgatcgc
     5041 aggccggtaa aataatacgg caaatttgtt tgtttgtaag tgaagtctcg atgacctaaa
     5101 atcctaattc tgaggcgatt cacctacatt caacgatgct tcagagacaa gaaaccaatg
     5161 aggtgtctaa tggagcgatt aaaaaacaag gtcgcattaa ttactggtgg tagcagaggc
     5221 atgggggccg ccgaagccaa aatgtttgct ggcgaaggcg ctcacgtagt catagcagac
     5281 atactagaaa atgaagggag aaaaacagca tctgaaatag ccgcctctgg tgcaagatgc
     5341 gtattcacac accttgacgt cctcaaagcc tcacaatggg attccgcgat aggcacagcc
     5401 atatcaattt ttggaaaatt agacgtgcta gtaaacaacg caggagttac atctagaatg
     5461 atgttactag acacctcaga accagaatgg gatcgagtaa tggatatcaa cacaaaggga
     5521 acatttttag gaataaaagc tgctatccct gctattcgtt attccggtgg agggtcaata
     5581 atcaatattt cttcgcaaat gggtttagta ggtgctgatt acataagccc ccagtaccaa
     5641 gcttcaaaag gtgctgtgag attattaaca aaatcagtag cgattcagta cgcctcggac
     5701 aacattagat gcaattctat acatcctgct ccgattgaaa cagagatgac tgcagacgtg
     5761 agacaggatc cggatgcctt ccaggatatg cttagaagaa tcccgatggg acgatacggg
     5821 aaacctgaag aggtggccta tgcggcttta tatcttgcct ccgatgaatc atcatttgtg
     5881 acaggtagtg agttggttgt agacggagga tggactgccc agtagtaaca aataaattct
     5941 gattggtaac acagaaaaga ggtctttatg aaaatcacag atattagtca ctacattatc
     6001 catccaggag tcggtaagaa tctatgtttc gtaaaaatcg acactgatga aggggtagaa
     6061 ggatggggag aatgctatac ccaagctgac cgagacaagc aggtagtttc acacatagat
     6121 caattgaaga gatatctcat tggtagaaac cctatgcata taaagcactt cttgcaaatg
     6181 tcttttgatg attttgccgg cagacgagga gctatggatt tttactgtgc aatcagtggt
     6241 ctagaacagg caatgtggga tatatcagga aagatatgcc aggtacctgt ttatatgctg
     6301 ctaggcgggc catgcagaga taagatcaga gtttatgcaa atggttgggg gggaggtaac
     6361 tgggattcgg atgagatagc cgaaaaggca aaaaagattg ctgatatggg ttttactgcc
     6421 atgaaatttg accctatccc tggcccttgg cgtactttcg tcgacaaaga ggtagaaagg
     6481 caggctgtag agaatgtcaa agctgttagg tcagctgttg gtccagatat tgacattctg
     6541 gttgaaatgc ataggaggct agccccaatg catgcagtaa agattgcaaa agaaattgaa
     6601 gaattcaacc cattttggtt tgaagaaccg gtacttgcag aaaatgtaga tgcattagca
     6661 tcagcaaaga aagtcatcaa cataccagta gtgaccggcg aggaactgta tacaaagttc
     6721 gaattcagag aaatttttga aaaacaagcc gcggacatta ttaatcccga tgtgtgtaat
     6781 gttggcggta ttctagaatt aaaggaaatc gctgctatgg ccgaacctta ttatgtcgca
     6841 gtgtcacctc ataattacaa cagtactact ctaggtcttt cagctacaat ccaagtatcg
     6901 gccgcaatac ctaattttct aattaccgaa tatttcgtaa attttgaaaa tcttggaaaa
     6961 gacatatcta cacacccctt tgaggtagat aatgggtaca tcaaattacc cacagaacct
     7021 ggacttggca tggatcttat agaggaaaat ttagaaaaat atccctataa acaatttcct
     7081 cccagaaacc tggcacaata ttcagatgaa gggccatagc ggcaaactca atatcattaa
     7141 ccattgtgga taataatcca atttaaagat ttcttgaccg aggtttagtc gcatgctagt
     7201 ttttatatcg aatatgaata agtagtgggc ggctaggtaa aatagttttc caaaaacctg
     7261 aacctaaagc actaagacta gtaacattgg cgcatgggat tatcgcaaga actaaatata
     7321 aaaaaggatc taagggattc gtttattact ataggtgtat tcgatggagt ccacctgggg
     7381 cacagacacc tagctaaaaa actcatagag tcagcgatga agaacggaaa atcatcaggc
     7441 atcataactt tcaaaaacca ccccgccgcc gtgataaatc cacgatttca accaaatttt
     7501 ttaaccagct ttgaaactaa gttggatttg ttgaaaaata caggagttga tttcgtatcg
     7561 gcaattacat ttgatcataa agtggctgat ctaacggccg aagacttcct gagcctacta
     7621 aagaatagat tgggaatgaa agggttagtt gtaggtcctg actttcaaat gggtaagaat
     7681 agagaggctg atactgatag attggccacc ttgggatcaa aattaggatt tgaattggaa
     7741 gtggtggatg tgcaaacaaa gggaggagat caagttcgga gtaccatgat aagaagtctc
     7801 atatcagacg gcgatgtgaa aaatgcctca gtcatgttag gaagaaaatt ctctctggaa
     7861 ggcagcgtca tcacaggaat gaaaagaggt aagtcactag ggtttccaac agctaattta
     7921 aaaaccaacg aaggcattgc tatcccgtcg aacggcatat atgccaccgt tgcaacgatt
     7981 aagggagtac gatacccagc agccacaagt gtaggaacca accccacttt tcctgggaaa
     8041 gaacaaacta tagagaccta catattggat ttcgacaaga atatatacgg acagagtctg
     8101 tctatatctt tttatgagaa acttagaaat gaacttacat ttgatagcgt tgataatcta
     8161 attgaacaaa tgaaatcgga tgtatcagaa gtaagaaaac taaacaaatc tggtgaactt
     8221 caatgaaaac tcacactcca cccttgttac catttatcct gatctgaaaa tataaaaacc
     8281 acaaacttaa ctaaaggtat ttcaaatgcc tctcacggaa aaggaagaca tacttagtcg
     8341 gggtgtcgaa gagataattg ttaaatccga attcatcgat agattgaatt cagggcaaaa
     8401 aatgcgtctc aaaatgggat ttgatcctag cgcgcccgac attcatgtag gacatgccgt
     8461 aggtttgagg aagctcagac aattacaaga aatgggccac caagttgtat tgatagttgg
     8521 agattggaca gcccaaattg gcgatcccag tggaagatct caaacaagaa aaataataag
     8581 tattgaggaa gttcattcca acgcacagac atacctggac caatttttta aaatcgttga
     8641 caaagaaagg acagaaataa gatggcagag tgaatggttt ggacctttca ctctaactaa
     8701 tgtaatagaa ctaaccagta aatttacggt agcccaaata ttggctaggg aagattttgc
     8761 aaaaagattt ggagaaaacc aaccaatatc tataacagaa ttattgtacc cattgcttca
     8821 gggatacgac tctgtagcca tcgaatcaga tgttgaattt ggtgggacgg atcagaaatt
     8881 taatttgctg gtgggaagag aattgcaaca aatgagaggt atgaaagccc aacaatgttt
     8941 cataatgcct ttgcttccag gaaccgatgg tgtccaaaaa atgagtaaaa gccttggaaa
     9001 ctacattggc atagatgaga cgccgcaaga aatttttggc aaggtcatgt caattccaga
     9061 ttcattgata ttaccttatt tcgactggct tacagacact cctacaaagg aaattactga
     9121 aatcaagaat tcattggaaa cccaatctgt taatccaata gaacttaaaa aaaatgtggc
     9181 atataaattg gtaacagact tccatgatgc aaaatccgca agttcatcta gggatttttt
     9241 tgaaaagacg gtccaaggag gccaggctcc agatgatatg ccagagtacg tcattccttc
     9301 ggggaatgct gccgcaggcc ttaagttgag taatattctt gtcgctagtg gactaacgtc
     9361 cagttctgga gaagccagac gcctcataga acaaggtgcc gtcaagatca atgaaattca
     9421 aataacccaa aaccaaccag taaaccggct tgagaaagga atccttagag caggtcgaag
     9481 aaggtatgtg aggctaatat aaaatagata ttcaccttat ctgttattca agttgacccg
     9541 ttgctgtgat aaaattctcg agcttgtttt tcatatgaaa tttcagcatc aatagctgat
     9601 gcggaggaag aaattggtta atctcagaat ccccggccca attcctgtaa acgacgatat
     9661 attggagtct atgggcaccc caatgataaa ccatcgaggt cctgaattta aggaaatttt
     9721 atatagaact acagaaaggc taaaaacggt tttctctaca aagaatgatg tctacataat
     9781 aactggttcc gggacatcag caatggaagc ggctgtggta aacactttgt cgcccgggga
     9841 caaagcgatt aatgcaactg ttggagtttt cggcaacagg ttcggagtta tatctgaacg
     9901 atatggtata gatctagtga gtttggaatt cccattcggt tcggatgtgg acttagatgt
     9961 cttacgcaat tgtcttaacg caaaccctga cgtgaaagct gtaatggtca cccacaacga
    10021 aacttccact ggtgttacaa acaacctgaa aggcatctct gatgtggtta agaaagaata
    10081 cgataaatta attttggtag acggaataag cagcgtttgc tctctaccgc ttgaaacaga
    10141 cctctgggat ctcgatgtag tcgcaaccgc ttctcaaaaa ggttggatgc ttccaccagg
    10201 actggcattt attagtttta gcgaaaaggc atgggatgca cactcacggt ctactatgcc
    10261 cagattttat ttagacatgg ctgagtacaa acggtactac gagataggcc aacccccttt
    10321 cacgccatca gtttcatgta tgttcgctct tgatctagct cttgaccaaa ttattacaga
    10381 gggcatggga tcggtctttg aaagacacgc atcgattggc caatacacaa gggattgcat
    10441 agcagaattg aacctaaaca tattccccca gagtaataag atagcatcca atacttgcac
    10501 tgcggtggaa gttcctgaag gggtagacgg atctaaactt gtctctaaaa tgagggatga
    10561 gcacaaagtg gttctagcag gaggtcaggc tactctcagt ggaaagattt tcagaattgg
    10621 tcacatgggg caaacaacag aatcagacat aaaagaggcc atagacgctc taaaaatcgt
    10681 tctgccagaa attgggttca agaattagta attaactatc tgttaatttg aagccactgc
    10741 tgaagcttca tccaaaagct tctttaagtc gtccccttct atagtttcat gttctatcaa
    10801 atgagatgcc aatgcggtca atttgcccat gttagtatcg attaaatttc tggcaatttc
    10861 ataggcatgc tgtatcaacc catgtacttc ttcgtcaata gtctgagcta ctgtatcgct
    10921 atagtctttt tgctctgaaa tttctcttcc cagaaatacc aattcttctc gtttaccaaa
    10981 agtcctgggc cccaatttct tactcatacc caaccttgta atcatagccc ttgcaatatt
    11041 tgtggcttgc tccaaatcgt tgcctgctcc tgtggtcacc tcaaaaaata ccaattcttc
    11101 agctactcta cctccgagcg ccgatgccag ccttgcctct aattgatttt gggttacgag
    11161 ttgagtttct tcttctggta taaatcgagt gtgaccccca gtttggcctc tggccactat
    11221 agttacttta aagggagggt ccgcgtgtgg aagtagatgg gccaccaagg catgccctgc
    11281 ttcatgatga gccaccatgc tcttttctct ctcactgacc actttattct ttctttctgg
    11341 accagctatc acacgatcaa tggattctgc catctcatca tatgtaattg tctttttatc
    11401 tcgcctagca gccaagatag ctgattcatt aagtaagttc atgaggtcgg ccccactaaa
    11461 accagcagtc tgcttagcta ttgcctccat gtcggtatcc tcagaaatcg gcttaccttt
    11521 tgaatgaaca ttcaatattt cttccctacc cttcacatct ggactatcca aagttactct
    11581 tctatcaaag cgaccaggcc tcaacaaagc tgggtcaaga atatctggtc ggttcgtaga
    11641 ggccaacaca ataacattcg ttcctgtgtc gaaaccgtcc atttcaacca atatttggtt
    11701 caatgtctgt tctctttcat catgccctcc acccagcccc gcacctcgat gcctaccaac
    11761 agcatcaatt tcatcaacaa aaacaataca gggggcgtgc ttttttgcct gatcaaataa
    11821 atctctgact cgcgatgccc caacgccgac aaacatttca acaaactcgg aaccacttat
    11881 ggaaaaaaaa ggaaccgctg cttccccagc tacagcgcga gccaataagg ttttaccaac
    11941 accaggcggt cccatcaaca aaactcctct gggaatcttg gcacccaaag ctaaaaaccg
    12001 ctctggagat ttcaaaaact ccacaacttc ctgcaactcc tccttagcct catctacgcc
    12061 agcaacatca gagaaactta cagtaggatt atcgctatca aaaacccttg ctttcgattt
    12121 accaaaacta aatgtttggc tagtgctacc ctgcgcctgc ttcatcatga agagtaatat
    12181 ggctccaaaa aaaataagtg gtagaaaatt aattaggatt ccaaaaaagc tcccaatacc
    12241 accagcccca cggacagata cgtccacacc tccagcaccg cttcttactc cggccacttc
    12301 caactgttcg acaatactcg agcccgattc cttcctcgaa gtatactgac taccatcact
    12361 tccaacggcc gttaagctat ccccacttat ctcaatcgat tccaatttat tctctgatgc
    12421 gagcgatata actctactta ttggtatttc tgaagagcct ccaaatggct ccttaaaaac
    12481 cgtcaaaaat atagccatta ttccgacagc tatcagtaaa tatataaaca tgcttctcat
    12541 ggaacgcata ttcatctaac gttctccata tattcttaca cttggataat ttctaaactg
    12601 taccgttttt ctttatcaga ttagggcctt caatagtaca ttacacccct gcgttggagc
    12661 aaagatttaa ggttcagtat caacacaaaa attcacgttt gctataatct acaacctcgg
    12721 aatagcagac tatttaaaac agactttcat aagtctgtta cctttctttg tgtaaacgaa
    12781 taatttgatg ttggtgaaat acgctttaag gctcaagtga aatgaatata gcccacaaca
    12841 aatctaacgg cttaaccaga gaaaacattt gccagcttct agctgaagag atggtgacac
    12901 cgatatttgt gtctaattcc aatgagtccg aaactttcat gggtcaagtc gacatcgccg
    12961 aagccagtcc tttaatagtt atggatttag gcttcctcaa tagggcacaa acgtccaaga
    13021 tatcggaatt atgcaaagaa aaaaaccttc caatactggc aataatttca tctgaagacc
    13081 taatgtcact tgataccgat atgataatta cagattttat tgttgcccca ttccctgagg
    13141 aagaattaat tgtgaggtca atcttttctg taaaaagggc caccaccggt cccttaaagg
    13201 aagagttaat aaatagagga gaccttacga tcaatccctc caattatgag gttttgataa
    13261 acaatagcag gattaatctg aggtttaagg aatatgaatt gttactactt ttggcgtcaa
    13321 accccggtcg ggtttatgac cgagcaacac ttttaaacca aatatggggc tatgattatt
    13381 ttggcggaac ccgaacagta gatgtacaca taagaagact aagaagcaaa atagagaata
    13441 atccagaaaa cccttacatt gaaactatct ggaacgttgg gtataggttt agatcctcta
    13501 gctaggcgtt accgttcatc taccttcagt taaataattt tgacctccct ctgatatcct
    13561 caatataatg ccgttagaaa tttgaacatt tccgtatcga tgatataggc aaaaaattgg
    13621 ctgaaaacaa gcaatgaaca caaacttgaa aattaacgat gccttgcttc tacagatgta
    13681 caaagagatg gttctctgtc gaactctcga cgagcgtatt tggatgctaa atcggcaagg
    13741 taaagcagct attatggctt cttcccaagg acatgaagca gggcagattg gttcagtggc
    13801 ggctctccgg aaaggatacg atcaatgtta catttactat cgggatcttg ccgtgctcct
    13861 cacacttgga ttaaccccag ctgaaataat taaaggtttt atcgcgaaaa aaggagaacc
    13921 attaagcgga gcaaggcaat tcccgaccca tggtgcccat cctacctatg gtgtaatcaa
    13981 tctctcaaat gtcatagcaa cacatatacc tcaagctgtt ggagcagcat taactgcaaa
    14041 gataatgaag gaggacaggg tcatagtggc gtattttgga gatggcgctt ccagcgccgg
    14101 tgattgccac gaagccatga attttgctgc cattcacaag cttccagtca tatttttttg
    14161 tgaaaacaat ggatacgcga tatctgttcc attatctaaa caaatggctg tggataatgt
    14221 cgctgcgagg gctgaagggt acggaatgcc gggaatagaa attgacggct gcgatattgc
    14281 agcagtatac gagaccacca tcgaagcagt tgccagagcc agggccggct tagggcctac
    14341 tctaatagaa gcaaatgtag aaagatatct tcctcatacc agtgacgacg acgactctat
    14401 atatcgatct tctcaagaga ttgaagaagc aaaaaaaaga gatcccttga agttacttcg
    14461 cgaacagctt ttggaacgaa agataatgaa cctaaaagaa gatacatata tccacgaaga
    14521 tgccaaagat atcgtaaata aagcaacgga agaagcagag aatgccccgt accctgaaac
    14581 tgtcgacttt ttcaaacatg tgtatgcgga gtagataaat gaaatggctg taatcaccgt
    14641 attagaagct attagggaag ccatgcgaga agagatgcga aacgatgaac gggtgttcat
    14701 catgggggaa gatattggcg ccaggggggg agtatttctt gccaccgacg gtttcctatc
    14761 tgaattcggt gaagaaagag taatagacac acctcttgcc gaatcttcaa ttgctggaat
    14821 cgccttagga gcagccatga gtgggctccg acctattgca gaaatagaat tcgctgattt
    14881 tatctggccc actataaatc aaattgttgg cgaggcagca aaggttcggt atggtacaga
    14941 aggtaaacta aacgcaccgc tggtgctgag agccccgcaa ggtggaggtg taaggggagc
    15001 cctataccac tctcaaagcg ttgaagctct ttttgcacat acccccggtc taaaagttgt
    15061 gacaccttcc actccgtacg atgcaaaagg cttgttaaag tccgctataa gagacgatga
    15121 ccctgtaatt ttcctcgagc acaaaaggac ataccgactc gtgaaaggtg aagtcccaga
    15181 acaagaatat acaattccca taggaaaagc cgatgtaaag aaacccggtg aagacattag
    15241 tataattact tatggtctaa tggttcatca ttgcttagaa gcagcctatc tagccagttc
    15301 agaaaatatc agtgtagaag ttttagattt aagaacagta agacccttag atatagaaag
    15361 tatcattggc acagtggaaa aaaccggaaa ggccttgata gtttatgagg acaaccaagc
    15421 actcggaatc ggatcggaag tagcggcttt gatttcgcaa cacgcatttg agtacttgga
    15481 tgcaccagtc attagattag gtggccccga agtcccagca atgcccttca gcccaccact
    15541 agaagatatg tttatgatag acccaggaaa aatatcaaca gcaatcaaag aattagcaga
    15601 gtattaggta tcatgaagat cgaacttccg caggtaggcg aatctgtcac agaaggaatc
    15661 ataggcagat ggctaaaaaa tgtcggggac aaagtagaaa agtatgaccc cttggttgaa
    15721 gtcataaccg ataaggtcaa tatggaactt ccctccccag tgagaggagt tcttcggaaa
    15781 ataatcgcta atgaaggtga aactgtccta atgggtggtt ttattgctga cattgaaaca
    15841 agcgaaaatc aagaaggtgc taatgactat aaacctgaac cagatacaac aggtcaggaa
    15901 attggcacga ctggtgttct tctaaaaaat acagctccag tcggaccaac tgggtctgga
    15961 ggtccgatcc accaggatga atccaaatca actcctgata aaaaacaact ctcaaaaggt
    16021 aggtattctc cagctgtttt aaggctagca gaggagaata aaatagattt atctcaaatt
    16081 gctggcactg gaattaacgg aaggatcacc cgtaaagacg tagccgcatt cattgaatca
    16141 gatctgaccc cgccacaaaa caaagaattg ccaccaatgc cagcaaacga caccaacccc
    16201 aggaaagatc aagaaaaaat ttcccttagc ccggtgagaa aaatgatagc ggctaacatg
    16261 gtaaaaagcg caactttaat accccaagct tggagtgaag tagctgtaga tgtcacgggg
    16321 atggtattga aaagacaaag catcaaaaag tccttcatgg ataaagaagg catcaacatc
    16381 acctacttgc cgttcgtagt aaaggcagtt gccgaatcac taaagaaaaa tccattactg
    16441 aattcatcat ggtcagaaga aggaatcatc ctgaaaaaaa acattaatat tagtatagct
    16501 gttgcggccc ctggcgggct ggtagttccc gtcatacaca acgctgatct attcagcata
    16561 gccggaatag ctggaattat agaaaatctt gtaaccaagg caaaaaatgg taagttggac
    16621 ctacctgaag tccaaggtgg tacattcact ttaaataata caggcgcctt gggatctgta
    16681 tcttccaggc cgttagtgaa tcatcctcag gcagctataa tgactacaga ggcaatagtc
    16741 aaacatccgg tggtcagaga ggacgccata gccatcagat ctatcatgaa tatctgctta
    16801 tcttttgatc acagaataat ggatggagca gaagcaagtg cattcagtat gactgtgaag
    16861 aacttgctgg aaactataaa tccagaaaca ccaatttact aacctggggc cagaatgctg
    16921 actacatact atggtttagt caggtatgac ataggtcaag ccattcagca gaaaactcat
    16981 gctaaagtag ctgcaggagg agacgatcaa gttctcattt ttcaacaccc aaatgtatat
    17041 actttgggga ggatgggaag gttggaggac atattaatag aaaccgatgc cattagacgg
    17101 ctgaatcttg aaatccgaaa taccgatcgg ggtggtgaag taacctacca cggtccaggt
    17161 cagctggtag tttacccaat aataaaatta gatcggattg aattaacacc atctgcatat
    17221 gtcaattcac tgaaggtttc tatcgctgaa tgtcttgctg attacggcat catcacgaac
    17281 gagaaaaata tgccgaccgg agtctgggtg gaagatagaa agatcgccgc tattggagtc
    17341 aggataagca aaaaaaccac ctcacatgga ttggccttaa atgtaaccac agatctatcc
    17401 tatttcgatc acattatccc ctgcggcata cataattgcg ttatcacttc cattaaagaa
    17461 gaaattggag atcacctgaa aattgaagat gtcgctcaca aactcgcatg taaattagga
    17521 aaggctttgg gcaatcgaat aacctggaaa gacgattcac ctaatcagga ttctgacccg
    17581 tcaccagcca tataacgcgt tccgacatat ttgttgttct gtctgcgacc ctctcgaggt
    17641 catgagccac ccaaattagg taggtagccc tttgaatggt gctcggatcc gccatcataa
    17701 aagtcaagag ctctcgatat acttggtggt aaagttcatc gactacgtca tcatcatccc
    17761 gaacagacgt agccaaggcg gtatctctat ttatgaaagc ttccgtactc cttctcaaca
    17821 tatcggttgc aaggtcagac atgcgtggta tatctataag tggctttaat ggcgggagat
    17881 ctcccattgc caaactgatt ttgccaatac cttcagcata atcccctatt ctttcaagtt
    17941 ccgtggccat catcatcacg gatataatta ccctcaaatc tcctgccata ggcgcttcca
    18001 aagctatcaa ttcaatgcat ttatcttcaa tatcttgctg cttctcatca atggcatcat
    18061 catcatctat aacctgctga gaggcattaa tatctctgtt acgaagggca tcaattgact
    18121 taaaaaccgc cttctctacc atactggata actgaattaa ctcatcctgg acaaccttaa
    18181 gattgttttc gaaatcttct ctcggcattt ctcacccctc taactgaatc tacccgttat
    18241 gtattcttct gttctctgat gcgtaggatt cgtgaatatt ttttgtgtat catcctgttc
    18301 cacaagatac ccagaacgat cttctcctac catgaagaaa gctgtttcat cagataccct
    18361 cgccgcttgt tgcatattat gggttactat aacgattgta tagtcagtct taagttctct
    18421 caccaaatct tcaatagcta aagtagctat tggatccaat gcagaggcgg gctcgtccat
    18481 taaaattacc tctggttcaa cagcaagggc acgagctatg cataatcgtt gctgctgccc
    18541 accagaaaga gtgaaagcgc tatcattgag cctgtccttc acttcatccc acaaagcagc
    18601 ctgctttagt gatttctcta cagcttcttc catgtcaccg gaaaaaccat tcaccttaag
    18661 accaaaagca atattctggt atatagattt agggaaaggg ttgggttttt gaaaaaccat
    18721 gccaattcta gatcttattt ctgttgcatc cacatttgag tcatatatat tcctattttc
    18781 aaataggact tccccttgga ttgaggcgcc cataatcaaa tcattcattc tgtttaagca
    18841 tcgcaagtag gtgcttttac cgcatcctga aggcccaatt aaagccgtaa cactttctct
    18901 tttaatctcc atatctacgt cttttaccgc ctgaaaatct tcgtaataga cattcaaccc
    18961 tttaacactt ataacagtac tgtctgccaa tttattcctc cgatcgggtt tgatatttat
    19021 ttcttaaaaa aaccgctata gcgttcattg ttagcaatat tacaagtaat actattattc
    19081 ctgcagcagc cagtgatcga aaatcatctt gaggccttgc gacccagttg aatatctgta
    19141 tcggcagcac agtaaaacgt tcaaaaggat gagctggtat gtaggttata tacactagag
    19201 ctgtaatagc aataactgga gccgcctctc caatagctcg agacattgca agaattgtcc
    19261 ctgtcaagat tccagaaaat gccgaaggta atactgcccc tttaatgacc tgccatttgt
    19321 ctgcccccat agcataggcc ccctgacggt atgaatcagg aacagcacga atagattctc
    19381 tcgaagcaag aataacgatt ggaagaacca gcaag
//
LOCUS       DASCNS010000014         6623 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000127217, whole genome shotgun sequence.
ACCESSION   DASCNS010000014 DASCNS010000000
VERSION     DASCNS010000014.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000127217"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="QF838_00485"
     CDS             <1..743
                     /locus_tag="QF838_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HJO59204.1"
                     /translation="VDIVIECTGKFTDSAKASGHLEGGAKKVIITAPAKNEDITIVLG
                     VNEHQYDKTNHHIISNASCTTNCAAPLMKVLDDSFGIEQGMMTTIHSYTNDQQVLDKR
                     HKDKRRARAAAMNIIPTTTGAAKAVGQVIPNLEGKVHGMAFRVPTATVSVTDFVATLK
                     TRATAEEINLAYQEVSQNRLNGILEYSNEPLVSSDYRESKYSCTIDGLSTITLDTNMA
                     KVVGWYDNEWGYSCRTVELAKLVAEAGF"
     gene            841..1740
                     /gene="pdxS"
                     /locus_tag="QF838_00490"
     CDS             841..1740
                     /gene="pdxS"
                     /locus_tag="QF838_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HJO59205.1"
                     /translation="MATEIKPNTGTWTVKSGLAQMLKGGVIMDVVTPDQAKIAEDAGA
                     VAVMALERVPSDIRADGGVARMSDPQMISEIIEAVTIPVMAKARIGHFVEAQVLEALG
                     TDYIDESEVLTPADENNHINKHDFSVPFVCGARDLGEALRRIGEGAAMIRTKGEAGSG
                     NIVEAVRHARKVLGQISHIRGMESDELMATARDLKAPYELVKGIHQSGALPVVNFAAG
                     GISTPADAALMMQIGVDGVFVGSGIFKSETPQVMANAIVKATTHYKDPEMLAAVSKGL
                     GASMPGIDVRTLPESEQFATRGW"
     gene            1743..2321
                     /gene="pdxT"
                     /locus_tag="QF838_00495"
     CDS             1743..2321
                     /gene="pdxT"
                     /locus_tag="QF838_00495"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HJO59206.1"
                     /translation="MAPLGVLAIQGDFLEHRNMLDSIGIESVEVRLPEQLENIDGLII
                     PGGESTTIVQLIDIYEMRQPLIEKVKNGLAVWGTCAGMIVIAKHLKDKRPDPLGLMDI
                     EVSRNAFGRQIDSFETDLDINGIDGPPIHAIFIRAPLVTEIGNGVEILAEVGDGKPVA
                     VKQNKILATSFHPELTEDPRMHALFANIAHSM"
     gene            2348..3925
                     /locus_tag="QF838_00500"
     CDS             2348..3925
                     /locus_tag="QF838_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HJO59207.1"
                     /translation="MIRETDIDLNVLVDVIPERFRSELLAQENVGELLEVVLDLGRQP
                     EARFLGQNKLLEIDEVTKDDLEYAISKIGVFGADNRAGIERTLHRISAIRNRSGEVVG
                     ITFRVGRAVYGTIKIIEDLVLSGESVLLLGKPGVGKTTMLREVARVLADDAGKRVIVV
                     DTSNEIAGDGDIPHKAIGRARRMQVPYPTKQHDVMIEAVENHMPEVIVIDEIGTELEA
                     NAARTIAERGVQLVATAHGNTLENLIMNPTLSDLVGGIQTVTLGDIEARRRRTQKTVL
                     ERNAPPTFEKLVEIQGWNLVTVHSEVALTVDKMLRKQTINSEVRAVEENGNIRKHTSS
                     FKSDEGPQSTDFPNETVEPYRRANSTIPTETAKLTPIMPYGVNKSRFDQVIRSMNAPI
                     TLVADINKAEMLVTTKNYHRRGTQALKSAEKMGKPVFVLRKNTVHQIEQFVKAVTEND
                     TGPMVSESEKSLALLEAEQASQRISGGELKILLRPQNSFVRRLQHQIATEYGLESSSE
                     GKEPRRSVLIHKDTDEY"
     gene            3915..4532
                     /gene="tmk"
                     /locus_tag="QF838_00505"
     CDS             3915..4532
                     /gene="tmk"
                     /locus_tag="QF838_00505"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HJO59208.1"
                     /translation="MNTKNAFIVLEGVEGSGKSTQSQLLSQKLSSEGVSTLLVREPGS
                     TNTGEKIRALILEGSELNPLTELFLFSAARSELIQNEIIPALDSNTIVVCDRYIYSSI
                     AYQGYAKGLSLDTIDEVNSIATSHISPDLVFLLDINPKVAFERKKSDPKDRFERESMG
                     FHNSVRKGYLSLAESNQNAWHIINAEKPTEIISEKIWQKVITFLT"
     gene            complement(4534..5262)
                     /locus_tag="QF838_00510"
     CDS             complement(4534..5262)
                     /locus_tag="QF838_00510"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HJO59209.1"
                     /translation="MIDMYDIHNHLLPGVDDGAEDLLESIQICEIAQNQNTKMIIATP
                     HRKDVIENHSVTLVEDLVSKINENLTQKKADMDVKLGMENHVDLDLIRDIENGRALTL
                     NNSEYILVEMPWEGRPLYIEQVIADLLQSGWIPVLAHPERMRLFENERQLLWEFVQMG
                     CKSQLTASSLYGKFGEKAQRASVDMLTEGLIHVIATDTHMAEGQRCYDIAKGYKFAEQ
                     IIGSYRATQMVQSNPLAIFENQKM"
     gene            5340..6245
                     /locus_tag="QF838_00515"
     CDS             5340..6245
                     /locus_tag="QF838_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO59210.1"
                     /translation="MIKFDNFSTAYQKKIVEFINSVNDSFDSPKKLTDAFFRQTINVP
                     PSIPRKDCYIASINNDILGFLQIVEEKPVSRIVGLQTIREGSNFDEIFQNFINIAVKH
                     ATVTKAKVIHFQISQNDHQSNTILESSGWHKIKTYWNLRFKQPQLEDLVLPDGYSLRH
                     FVKGKDENTLTDLQNQSFGKHWGFSPNDIEQIRYRSQMERTDEEGIIFIAKNEEIAGY
                     NWTMEAHNGPSSIGWVAMTGVHPNYRGLRLGRAVVLAGMHYLKSKNISEIELEVDSEN
                     IPATKLYDSIGFKQINHTYWYEKKM"
     gene            complement(6248..>6623)
                     /locus_tag="QF838_00520"
     CDS             complement(6248..>6623)
                     /locus_tag="QF838_00520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJO59211.1"
                     /translation="GSIPHDALALYYSAADVFVLPSHYESLGFVVIEAMACGTPVVAS
                     RVGGIPSVVEHGSTGYLIPWRCPEAFASQIEVLLKNKDLHRFMSKEAMKKAYSLSWDA
                     SSEKAANLYSKVISGWINTQAI"
BASE COUNT         2132 a         1355 c         1449 g         1687 t
ORIGIN      
        1 gtgtcgatat tgtgattgaa tgcaccggca aattcactga ttctgcaaaa gcgtctggac
       61 atttagaagg cggggcaaaa aaagtgataa ttaccgcccc cgcaaaaaac gaagacatca
      121 ctatagtatt gggtgtcaat gaacatcaat atgacaagac caaccatcac ataatatcaa
      181 atgcttcatg tactaccaat tgcgcagcac cgctgatgaa agtgttggat gattccttcg
      241 gcatagagca aggaatgatg actaccattc attcttatac caacgaccaa caagttcttg
      301 ataaaaggca taaggacaaa cgtcgggcaa gggctgcagc aatgaacata attcccacta
      361 caactggagc tgccaaagca gtaggacaag ttatacctaa tttagaggga aaggttcacg
      421 gcatggcatt cagggtgccc acagcgaccg tatcagttac cgactttgtc gcaactttga
      481 agaccagagc cacagccgaa gaaattaacc tagcgtacca agaggtttcc caaaacaggc
      541 tcaatggcat cctcgaatat tccaacgaac ctttagtaag ctctgactat cgagagagta
      601 aatatagctg cactattgac ggtctttcca ccataactct ggacaccaat atggccaaag
      661 tagtcggctg gtatgataat gaatggggat acagctgcag aacagtagaa ttagcaaagc
      721 tagtcgcaga agcaggcttc taaaagtaag atcggttttc ttaattgaag ctttctagta
      781 tgaagagtta taatttgcat aagtgcatcc agtttgtata actgaaaacg gaggctatat
      841 atggcaacag aaataaaacc taacacagga acttggacag taaagtcagg actggcccag
      901 atgttaaagg gtggcgtgat tatggatgtg gttacccctg accaagcaaa aattgcagaa
      961 gatgctggcg cagtggcggt catggcacta gaaagagttc cctctgatat tagagctgac
     1021 ggtggtgtgg ctagaatgtc agaccctcaa atgatctccg agataattga ggccgtgact
     1081 ataccggtaa tggccaaagc aaggatcggt catttcgtag aagcccaggt tttagaggct
     1141 ctcggaactg attacattga cgaatctgaa gttttaactc cagcggatga aaacaatcac
     1201 attaacaaac atgacttttc ggtacctttc gtgtgcggtg ctagggactt aggagaggct
     1261 ctaagaagaa taggtgaagg cgctgctatg attagaacta agggagaggc cggctcgggg
     1321 aatatagtag aggccgtccg acatgcgcga aaagttctgg gacaaattag ccatattaga
     1381 ggtatggaat cggacgagct catggccacg gcccgagatt tgaaagctcc ttacgaatta
     1441 gtaaaaggca tccaccaaag tggagcctta cctgttgtaa acttcgcggc tggcgggatt
     1501 tctaccccag cggatgctgc attaatgatg caaattggag tagatggtgt atttgttggc
     1561 tctggaatct ttaaatctga aactcctcaa gttatggcta atgcaatagt taaagctacg
     1621 acacattaca aagatccaga aatgcttgcc gccgtctcca aaggattagg ggcatcgatg
     1681 ccaggaatcg atgttagaac tttgcctgaa tcagagcagt ttgctactag gggctggtaa
     1741 atttggcacc tttaggcgtc ttggcaatac aaggcgactt cctagaacat agaaatatgc
     1801 tagattctat tggaattgaa tctgtggaag ttcgattgcc cgaacaatta gaaaacatcg
     1861 atggtttaat catccctggc ggggaaagta ccacgatagt ccagctgata gatatctatg
     1921 aaatgcggca gcctttgatt gaaaaagtaa agaatggact agcggtatgg ggcacctgtg
     1981 ccggtatgat agtcatagcc aagcatttga aggataaacg tcccgacccc ttgggtttga
     2041 tggacataga ggtcagccgc aacgcgtttg gaagacaaat tgatagtttt gaaacagacc
     2101 ttgatatcaa cggaatagac ggccccccga tacatgctat tttcattcgg gcgccactag
     2161 tgactgagat aggaaatggg gttgaaatat tagcagaggt aggtgatggg aaacccgttg
     2221 ctgtaaaaca aaacaaaatt cttgctactt catttcatcc ggaactaact gaagatccta
     2281 gaatgcacgc cctgttcgca aatatcgctc attcaatgta ataagacttg ggaggaccac
     2341 agcaaaagtg atccgagaga cagatataga tctaaacgtc cttgtggacg tgattcctga
     2401 aagattcagg tcagagttgt tggcccagga aaatgtggga gaattgctgg aagtggtcct
     2461 agatttaggc aggcaaccag aagcaaggtt tttgggtcaa aataaactgc tggaaatcga
     2521 tgaagtcacc aaagacgatc ttgaatacgc tataagtaag atcggagtgt ttggtgccga
     2581 taacagagcc ggtatagaac gtaccctcca ccgcatatct gctattcgaa accgtagtgg
     2641 tgaggtagta ggcataacat tccgggtggg gagggccgtc tacgggacta tcaaaataat
     2701 agaggattta gtgttgtctg gtgaaagtgt tctactcctg ggaaaacccg gggtgggaaa
     2761 gacaacaatg ctgcgagagg tagccagagt tttggcagat gatgcaggaa agagagtgat
     2821 cgtagtggat acctcaaatg aaatcgcggg cgatggcgat ataccgcata aagccatcgg
     2881 acgagccaga agaatgcagg taccttaccc gactaaacaa catgacgtaa tgattgaagc
     2941 tgtagaaaac catatgccag aagtcatagt tatagatgaa ataggcaccg aattagaggc
     3001 caatgcggcg agaaccattg cagaacgagg ggttcaattg gtagctaccg ctcacggtaa
     3061 tactcttgaa aatctaatta tgaatccaac gctatcggat ctagtaggtg gaatccaaac
     3121 cgttacactt ggagatattg aggctagaag aagaaggaca caaaaaactg tgttagaaag
     3181 aaatgctcct cctacttttg aaaaattggt agagatacaa gggtggaacc tagtaacagt
     3241 acattcggaa gtagccttaa ctgtagataa gatgttgcgc aaacaaacca taaattctga
     3301 agtgcgagca gtggaagaaa atggcaatat aaggaagcac accagttcct tcaaatctga
     3361 cgaaggaccc caaagtaccg attttccaaa tgaaactgtt gaaccatatc gtagagccaa
     3421 tagtacaatc cctaccgaaa cagcaaaatt gaccccaata atgccatacg gtgttaataa
     3481 aagcagattt gatcaagtca tcaggtctat gaatgcccct atcacattag tggctgacat
     3541 caacaaagca gagatgctcg ttacaacaaa gaattaccat agacggggta ctcaagccct
     3601 gaaaagtgcc gaaaaaatgg gcaaacccgt ctttgtgctc agaaaaaata cagttcacca
     3661 aattgagcag tttgtaaaag ccgttacaga aaatgatacg ggacctatgg tttcagagtc
     3721 ggaaaagtca ttagctctgt tggaggccga gcaggcgtca caaaggatat ccggcggaga
     3781 attaaaaatc ctgttaagac cacaaaactc ctttgtgaga agactccagc atcaaatagc
     3841 cacagaatat gggcttgaat ccagtagcga agggaaagaa ccccgtagat cagtgcttat
     3901 acataaagat accgatgaat actaaaaatg ctttcatcgt acttgaagga gtcgaaggaa
     3961 gtggaaaaag cacacagtcc cagcttcttt cacaaaaact ttcctctgaa ggtgtatcaa
     4021 ctttattggt tagagaacct ggaagcacaa acactggtga aaaaataaga gctcttatat
     4081 tagaggggtc cgagctaaat cctctgacag aattattcct tttttcggcc gctcggtcag
     4141 aacttattca aaatgagata attcctgccc ttgattccaa cactatagtc gtctgtgata
     4201 ggtacattta ctcatctatc gcatatcaag gctatgcaaa agggcttagc ttagacacta
     4261 tagacgaggt caattcgatt gcgacctctc acataagccc ggatttagtg tttttattgg
     4321 acataaaccc aaaagtcgct tttgaaagaa aaaagtcaga tcctaaggat aggttcgaaa
     4381 gggagtctat gggcttccac aacagtgtaa gaaaaggtta tctatcccta gctgagtcaa
     4441 atcagaatgc ctggcatata ataaatgctg aaaagcccac tgaaataatc agtgaaaaaa
     4501 tttggcagaa agtaattact ttccttacat gatttacatt ttttgatttt caaaaatagc
     4561 cagtggattg ctttgcacca tttgagttgc cctgtatgaa ccgatgattt gttctgcgaa
     4621 tttatacccc ttagcaatgt cataacaacg ctggccctct gccatgtgag tatcagtggc
     4681 aatgacgtga atcaatcctt ccgtcagcat gtctactgag gctctttgtg ctttctcacc
     4741 aaacttaccg tacaaactac tcgcggttaa ctgagattta catcccattt gcacaaattc
     4801 ccacaataac tgtcgttcat tttcgaaaag acgcatcctc tcgggatgag ccaagaccgg
     4861 aatccaacca gactgcaaca aatcagcgat gacttgttct atatataaag ggcgtccttc
     4921 ccacggcatt tcaaccaata tgtattcact gttatttaat gtcagagccc taccgttttc
     4981 aatatctcga atcaaatcta agtcaacatg attttccata cctaatttga cgtccatatc
     5041 cgctttcttc tgagtgaggt tttcatttat tttggagacc aaatcttcta ctaaggtcac
     5101 agaatggttc tcaataacat ctttcctatg aggcgttgcg ataatcattt tagtgttttg
     5161 gttttgcgct atttcacata tctgaatgga ctctagtaaa tcttctgctc cgtcatcaac
     5221 accaggtaac aaatggttat gtatatcgta catatcgatc atattattat gttacacctt
     5281 tcaaatattt ttcccttact ctgataaaat cctcccaaat aaacttttct tactgtgaaa
     5341 tgattaaatt cgacaatttt tcgactgctt atcaaaaaaa gatcgtagaa ttcataaatt
     5401 cagtcaacga ctcttttgat tctccgaaaa agttaacgga tgcttttttt aggcaaacga
     5461 ttaacgtacc gccgtcaatc ccccgaaagg actgttatat agcatctata aacaacgaca
     5521 ttttggggtt tcttcaaata gtagaagaaa aacccgtaag ccgaatagtc ggactccaaa
     5581 ccatccgtga agggagtaat tttgacgaaa tattccaaaa tttcataaat attgctgtta
     5641 aacatgccac ggtaacgaag gcaaaagtga tccattttca aatttcgcaa aatgaccatc
     5701 agtcaaacac aattttagaa tcttcaggct ggcacaagat aaaaacgtac tggaatctca
     5761 ggtttaagca accacaatta gaagatttag ttctacctga tggttattct cttcgtcact
     5821 ttgttaaagg aaaagatgaa aatactctta ctgatttaca gaatcaatca ttcggcaaac
     5881 actgggggtt ttctcccaac gacattgaac aaattcgata ccgttcccaa atggaaagaa
     5941 ctgatgagga aggtattata ttcatagcga aaaacgagga aatagccgga tataattgga
     6001 ctatggaagc acacaatggt ccgtcgtcga taggatgggt tgcaatgacc ggagttcatc
     6061 caaattaccg aggtttgagg ctaggtaggg cagtcgtttt ggcgggcatg cactacctca
     6121 aatcgaaaaa tatctccgaa atagaacttg aagtggattc tgagaatata cctgccacca
     6181 aattatacga cagtataggt tttaaacaaa taaaccacac ttactggtat gagaaaaaaa
     6241 tgtaggttca aatagcctgt gtgtttatcc aacctgaaat aactttactg tacaaattag
     6301 cggccttttc tgagctagca tcccaactta gagagtaagc ttttttcatc gcctcttttg
     6361 acatgaacct atgcaaatct ttatttttta acaacacttc aatttggctt gcaaaagcct
     6421 ccgggcacct ccaaggtatc aggtagcctg tggaaccatg ctcaacgacc gacggaatcc
     6481 caccaaccct agatgctact accggggtcc cacatgccat agcctcaatg actacgaaac
     6541 ccaaactctc ataatgagaa ggcaatacaa agacatctgc cgcagagtaa tagagcgcca
     6601 gagcatcatg gggaattgaa cct
//
LOCUS       DASCNS010000015         2629 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000155600, whole genome shotgun sequence.
ACCESSION   DASCNS010000015 DASCNS010000000
VERSION     DASCNS010000015.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000155600"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="QF838_00525"
     CDS             complement(<1..1222)
                     /locus_tag="QF838_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJO59212.1"
                     /translation="MENRTQTGREKDILQRAAKVLPQGSLGNLNYDLIVNRGKGSHIW
                     DESGNEYIDYLLGSGPMVVGHSNPYVMEVVLKQLNSGTTFFATNEKSVELAEEIVKAV
                     PCADKVRFCSTGSEATLYAMRAARAFTKKDKILKFEGGFHGMNDYALMSMSPSKLLDY
                     PQAEPDTAGIPKSLQSEMLIAPFNDLETTSAIVDRYSDELAGVIVEPFQRLLPPKQGF
                     LEGLREITSHYEIPLIFDEVVTGFRFSYGGAQDYYGVVPDICTLGKAIAGGFPLTAVA
                     GRDEIMTHFDSDKVGKEGFMPHIGTLNGNPVACAAGLATLNILKQDGVYDGIFDTGRK
                     VMEGLQDILDKSEIPGKVVGESVLFDVFFTSEEIYDYRSALKADSQKLSRFNQLLLEH
                     GVLKGSTKFYFSTEH"
     gene            1381..>2629
                     /locus_tag="QF838_00530"
     CDS             1381..>2629
                     /locus_tag="QF838_00530"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HJO59213.1"
                     /translation="MDINFQKGTPLEIVGDCLLVGLFEEEEFSSGELNRVNLALDGSL
                     RPLFDEGELVGKSGTSTLIHTLGKIAPKRILFTGLGKRDSVTEKVIKDVFSTAIRKTR
                     GIGLTRVVVAVDTFSSENIASKRVCELITLATINGLYRYEDHISEKSEKNIHEVILNT
                     DTSAEPNVTSYIAIADAINLARDLANAPPNFMTPRILSNIAEKEAKANSIGFERLDED
                     KMRELGMGSLLGVAQGSSEPAHLIVLKYIGDKDNPDDSIALIGKGITFDSGGLSLKPP
                     AGMITMKGDMAGGAAVIGALIAISKLALKINVYGIIAATENMPGGKAQRPGDVVTAMN
                     GTTVEVINTDAEGRLVLADALCYANHLGVSKIVDVATLTGAIRTALGDQIIGAFGNDD
                     EFTKEVIEAGAREGERLWELPADE"
BASE COUNT          786 a          559 c          570 g          714 t
ORIGIN      
        1 cgtgctcagt tgaaaaatag aatttggtgc ttccttttaa aacaccatgt tctagaagta
       61 attgattgaa tctagataat ttttgagaat cagcctttag ggcactccgg taatcataaa
      121 tttcctcaga cgtgaaaaat acatcaaaaa gtaccgattc cccaacaact ttgccgggta
      181 tttccgattt atctaatata tcttgaagcc cttccatgac tttccggcca gtatcaaaga
      241 ttccgtcgta aacaccgtct tgtttaagaa tatttaaagt cgccagtcca gctgcacagg
      301 ctacaggatt tccatttagg gtcccaatat gcggcataaa tccttctttc ccaactttgt
      361 cggaatcaaa atgtgtcatg atctcgtccc gtcctgcaac ggccgtaagt ggaaaacctc
      421 cagcgatggc ctttcctaag gtacaaatat caggtaccac cccataataa tcttgggccc
      481 ctccgtagga aaacctgaat ccagtcacga cttcatcaaa aattaggggt atctcataat
      541 gactagttat ttcccttagc ccttccaaaa aaccctgttt aggtggcaaa aggcgttgga
      601 aaggttccac aatgactcca gccaattcat cgctataccg gtcaactatt gctgatgtgg
      661 tctccaaatc attaaagggg gctattaaca tttctgattg gagtgatttc ggtatccccg
      721 cagtatccgg ctccgcctgt ggataatcta atagcttcga cggtgacatg ctcatcagtg
      781 cataatcatt cataccatga aacccgcctt caaatttaag tatcttgtct ttttttgtga
      841 aagcccttgc agctcgcatg gcgtataaag tggcttccga tcctgtgctg caaaaacgca
      901 ctttatctgc gcatggaacc gccttaacga tctcttcagc caattccacc gatttctcgt
      961 tagtagcgaa aaaggtcgtg ccgctattca actgctttag tactacctcc ataacatagg
     1021 gattagaatg cccaacaacc atggggccag atcctagaag gtaatctata tactcatttc
     1081 cactttcatc ccatatgtgg cttcctttac ctctatttac gataagatca taatttagat
     1141 tacccagcga accttgaggt aaaactttcg ctgctcgttg caaaatatct ttttcacgac
     1201 cggtctgagt tcgattttcc attttaaatc cttctcttcg tagactatcg tttttcacta
     1261 tcttcagggg attatatcaa tggacaactc aaaaaggatc atcctatggg taccgattca
     1321 gaaagtctta atgataaagt gagatggaaa tcacatacta cttaacaagg tgaaattaag
     1381 atggatataa attttcaaaa agggacccca ttagaaatag tcggggactg cttactcgtg
     1441 ggcttatttg aagaggaaga atttagctcg ggggaattga accgcgtgaa cctagcctta
     1501 gatggctctc taagacccct gtttgacgaa ggtgaacttg ttggaaaatc aggaacttct
     1561 accttaattc acacccttgg taagatagcc ccgaagcgaa ttttattcac aggcttgggt
     1621 aaacgcgatt ctgtgactga aaaggtaata aaagatgtgt ttagtaccgc aatcagaaag
     1681 acaagaggaa ttgggttaac cagagtagtt gtcgccgtcg atacgttttc ttcggagaac
     1741 atagcctcca aaagggtatg tgagctaatc acacttgcga ccattaatgg gctttacaga
     1801 tatgaagatc atatttcaga aaaatcagag aaaaatatcc atgaagtcat cctaaacaca
     1861 gatacctctg cggaacctaa tgtgacttct tacatcgcga tagcagatgc aataaacctg
     1921 gctcgagatt tggcaaacgc acctcccaac tttatgacac ccagaatcct ttccaatatt
     1981 gccgagaaag aagctaaagc gaacagcata ggatttgaac gtttggacga ggataaaatg
     2041 agggagttag gaatgggttc cttacttggg gtggcccaag gaagtagtga accggcccat
     2101 ttaattgttt tgaagtatat aggggataag gataatccag atgactcaat cgccctcata
     2161 ggaaaaggaa taactttcga tagtggtggt ttgagtctca aaccgccagc tgggatgatc
     2221 accatgaaag gtgacatggc aggaggagca gccgtaattg gagcactcat tgcaatatct
     2281 aaattagccc taaaaataaa tgtttacgga attattgcag ctactgaaaa tatgccaggc
     2341 gggaaagctc agcgtccagg ggatgtcgtg accgctatga acggaaccac agttgaggtg
     2401 ataaataccg acgctgaagg cagactggta ttagctgatg cattatgcta cgcgaatcat
     2461 ttgggggtaa gcaagatcgt ggatgtggct actttaaccg gagcaataag gacagcattg
     2521 ggggaccaaa ttataggtgc ttttggaaac gatgatgaat tcaccaagga ggttatagaa
     2581 gccggggcca gggaagggga aaggttgtgg gaattacctg ctgatgaat
//
LOCUS       DASCNS010000016         1797 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000168906, whole genome shotgun sequence.
ACCESSION   DASCNS010000016 DASCNS010000000
VERSION     DASCNS010000016.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000168906"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /gene="guaA"
                     /locus_tag="QF838_00535"
     CDS             <1..934
                     /gene="guaA"
                     /locus_tag="QF838_00535"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HJO59214.1"
                     /translation="DSVNRIREQVGKGKVICALSGGVDSAVAATLVHKAVGDQLTCIF
                     VNNGLMRRGEPDRVRETFEKYMDINLIFSESTERFLTGLAGVIDPERKRKIIGEEFIN
                     VFDEEASKLGVVDFLTQGTLYPDVIESKTSDNSVSATIKTHHNVGGLPDHMKMELVEP
                     LRYLFKDEVRQVGLELGLPEEMVFRQPFPGPGLAIRIMGEVTSEKLEILRAADWIVMD
                     EIKSNGLYNELWQTFAVLTGSRSVGVTGDFRTYGHVIAIRAVKSDDAMTADWARLPYD
                     VLAGISSRISNEVPDVNRVVFDITSKPPGTIEWE"
     gene            931..1203
                     /locus_tag="QF838_00540"
     CDS             931..1203
                     /locus_tag="QF838_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59215.1"
                     /translation="MKEEITFTNFRKEHGLEIIQLWRKSFAQAMRIEEDTREEVVNEH
                     LAYLRTYDPKIIRVALEKNSKIVGFMAKEENTIKDLFIHVEYQRRG"
     gene            1252..1443
                     /locus_tag="QF838_00545"
     CDS             1252..1443
                     /locus_tag="QF838_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59216.1"
                     /translation="MNTFQLNRGAQQFYEFHDFVIARVGFAGVEGNAWANSRGQLAGI
                     TLADITYEWKKLQSRYQEP"
     gene            1503..>1797
                     /locus_tag="QF838_00550"
     CDS             1503..>1797
                     /locus_tag="QF838_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HJO59217.1"
                     /translation="MVDNYSDEYLKQILAEVKSIAVVGASPSSHRDSFKVIKRLIDYG
                     YKVIPVNPNEVGNDIFGLCFHADLKSLDEPIDMVDVFRSSDAIMGIAEEAIEIG"
BASE COUNT          558 a          283 c          459 g          497 t
ORIGIN      
        1 agattcagtc aataggatca gagaacaagt tgggaaaggt aaagtgatat gtgctctttc
       61 aggtggagtg gattctgcag tagctgcaac tttggttcac aaggcggtcg gagaccaatt
      121 aacttgcata tttgtaaata acggtttgat gaggagaggt gagccagacc gggtaaggga
      181 aacctttgaa aagtatatgg atatcaatct cattttcagt gaatcgacag aaaggtttct
      241 taccggtttg gccggggtaa tcgaccccga gaggaagaga aagataatag gagaagagtt
      301 cattaatgtt tttgatgaag aagcctcaaa attgggcgtc gtcgattttt tgactcaggg
      361 gaccctatat ccagacgtaa ttgagagtaa gacgagtgac aactccgttt ctgcgaccat
      421 taaaacgcat cacaatgttg gtgggcttcc tgaccacatg aaaatggagc ttgtagagcc
      481 gctaagatac ttattcaaag atgaagttcg acaggttggc ttggaattag gactgcctga
      541 ggaaatggtt tttaggcaac cttttccggg cccaggattg gccattcgga ttatggggga
      601 ggtgacatca gagaagttgg aaattttgag ggccgcagat tggatcgtaa tggatgagat
      661 aaagtcaaac gggctgtaca acgaactatg gcagaccttt gcagtcctta ctggatctag
      721 gtcggtagga gtcaccggtg atttcaggac ctatggccac gtgattgcca tacgggcagt
      781 aaaaagtgat gatgccatga ccgctgactg ggctaggttg ccttatgacg ttttggctgg
      841 tatctccagc cgaatctcaa acgaagtacc tgatgtgaac agagtagttt tcgatataac
      901 gagcaaaccc cccggcacca ttgagtggga atgaaagaag aaattacctt tactaatttt
      961 agaaaagaac atgggcttga gataatccaa ctatggcgta aatcttttgc tcaagctatg
     1021 cgtatagaag aagacaccag agaggaagtg gtaaacgaac atcttgctta tctccgaact
     1081 tatgatccta aaatcataag agtcgcatta gaaaaaaata gtaagattgt tggctttatg
     1141 gcaaaggagg aaaacacgat aaaggatctc tttatccatg ttgaatatca aagaagaggg
     1201 tagggttctt gttttatacg ccaggccaaa gaggaagaag agtttctgac attgaacacc
     1261 tttcagttaa atagaggagc tcaacaattt tatgagtttc atgatttcgt aattgcaaga
     1321 gtcgggtttg ctggtgtaga agggaatgcc tgggcaaata gtagaggaca gcttgctggt
     1381 attactcttg ctgatattac ttatgaatgg aaaaaattac aaagtcgata tcaagagccg
     1441 taaaatattg attttattag ggttttagac aagataattt ttgagtgaga atgaagtgaa
     1501 gaatggttga taattattcc gatgaatact tgaaacagat actcgctgaa gttaagtcta
     1561 ttgcagtggt aggagctagt ccaagttctc atcgtgacag ctttaaagtt ataaaaagac
     1621 taatagatta cggctataaa gtaatacctg tgaaccctaa tgaggttggg aacgatattt
     1681 ttggactatg ttttcatgcc gacttgaaat ccttagatga acctatagat atggtggatg
     1741 tttttcgttc tagtgatgct attatgggta ttgctgagga ggctatagaa ataggag
//
LOCUS       DASCNS010000017         6165 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000171632, whole genome shotgun sequence.
ACCESSION   DASCNS010000017 DASCNS010000000
VERSION     DASCNS010000017.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000171632"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(90..1580)
                     /locus_tag="QF838_00555"
     CDS             complement(90..1580)
                     /locus_tag="QF838_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HJO59218.1"
                     /translation="MKLALLGIYHETNTFSKVVADYAAFNIYRKEEITREYQTSQTTN
                     AGFLQIGEDKDVEVVPLIFAITGPIGTITADAFESISKEMLSLLETHGPWDGVLLSLH
                     GAAVSEHYPDADGEIAERVRTLIGPDVKVGLSVDMHANLSRKMIENIDVATVYRTNPH
                     LDPKIRAFECAEMIRDSIKGKIDPVLWLEIPPVVINIVKQFTDEDPMKSVMEKLDKTL
                     SFPNIIHGSVAEGYPYADVEEMGMGFISVSDGDIEAAKEASQWMAREAWNIRDQFVGD
                     TPSIEDALSYADKKYINQEDGPYVLMDVGDNIGAGSSADSTHILSLAKSMGIKGYLQT
                     LWDPKSVKACVSAGVGSEVTVDVGAKTDDMHGLPVSVTGIVRTLFNGKFEDDRPTHGG
                     FKYYDGGLTAVLDTDDDHTLVLTSLRCGNTSREQMYSVGVYPEKYRVVVAKGVVSPRP
                     AYQPIAKEILLVNTPGVTTSDLNFFEYRMRRSNLYPFEDLASYDPD"
     gene            1689..3008
                     /locus_tag="QF838_00560"
     CDS             1689..3008
                     /locus_tag="QF838_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HJO59219.1"
                     /translation="MDREHSYDISGELYNRALNSLAGGVNSNVRLTETPHPLFYDKAK
                     GSKIYDVDGNQYIDYVLGQGPMIFGHSPDFLLQAVAEASESGQLFAGQHLLELEAAEA
                     VKSLVPNAELVRFASSGTEAVEAAFRLARAFTRRNVIIKFEGQYHGWSDGVLFNTAAP
                     LTPLDSNIFPEVEPMSEGIVDHSESALIILPWNNFEAIDAVFEQRGNEIAAVITEPIM
                     CNTNCIMPKPGYLQHLKKSCERNDSLLIFDEVITGFRVDAGGAQSLFGITPHLATFAK
                     AAAGGFPVSILTGSKEIMSLIGTGTVMHGGTLNANVMSMAATKSSMEHLSDRAIQTNQ
                     KLQDLGKRLMEGLEGINSRLEAGMLIQGPGSVFAVSFTDGREISDYRSHVENSDPDKY
                     ALFVSEMMKKGIRINSRGIWFLSESHTDEDIEATLSAASEVLQLIKL"
     gene            complement(3014..4030)
                     /locus_tag="QF838_00565"
     CDS             complement(3014..4030)
                     /locus_tag="QF838_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013595.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO59220.1"
                     /translation="MTTTQTEKSFLKYSHTIGICVMDGRGFMFPVDTAIGKNGRMHTV
                     SRAYAPAAAQLRITMYDIESEYFGIYGGYGESLGQFRWPTGMASDSMGNIYVSDELNN
                     RINVFSHEGDPIKYWGEAGSGEGELNGPSGIAFDSEGNLFVSDHMNNRIQKFTKDGKF
                     ILSFGNKGRNTLNMPWGVFVSSDGFVYVADWGNDCIAKYDSSGGFIRSFGNRGSEDGQ
                     FKNPSGVAVDKDGYIYVTDWGNERLEILDKNGNFIQTIRGQATISKWADEYFEGNYEE
                     AEPRSRSNLEPNPVQFGGDPHEESAHIEKLFWGPTSVKLDDEGRVYVTESNRHRVQIF
                     ERAV"
     gene            complement(4050..5495)
                     /locus_tag="QF838_00570"
     CDS             complement(4050..5495)
                     /locus_tag="QF838_00570"
                     /inference="COORDINATES: protein motif:HMM:NF020390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO59221.1"
                     /translation="MVSASTDVKLMAHLLRRAGFGATKEQLDQYISIGYDRVVDSLID
                     NEDSSRIDNALVRRYFPDQSVTHDTTGAGSYWLYRMVSTDSPLREKIALFWHNVFATG
                     YTKVTNGKPMSDQLRMFRSHGLAKLDELLLKLSRDPAMIIWLDNVDNHNGAINENYGR
                     ELLELFSMGVGNYNEDDIKECSRAFTGWSIANSDYIKQLAVRNSIMPYGKLAWRYEYK
                     EEDHDEGEKTFLGHKGNFNGEEIIDIICAQPATARFISRHLYHFFVADEPPVPSWPYK
                     EPQDLAAISILEKAYFDSGYDIGEMIRVLFNSDFFKSDTCHYKKIKSPAELAGNVLRT
                     TEEFGTPNIRISERNSQITFMGQQLLNPPSVEGWHQGLEWIETGSLTERVNFASQHLG
                     DLSKPGVRRIVQNVMDDEGECISASVCVDKCLDQLGAIQTSQYIYDTLVEFAQENGIP
                     SINLVKDKENAAKKISELMSVVASVPEFQQS"
     gene            5647..>6165
                     /locus_tag="QF838_00575"
     CDS             5647..>6165
                     /locus_tag="QF838_00575"
                     /inference="COORDINATES: protein motif:HMM:NF023568.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59222.1"
                     /translation="MPFAKLPDSNIHFVRKGSGEITYVFCHGLGGSGSRFEKEDIDWY
                     AEHFDVISWDNRGLGRSGASEKYSLPLYAMDLKNLLHYLKIEKAVIFGVSWGGVLAQR
                     FATMFPETCLALVLDSTSSEVNVKASENWYARGEIARLGEQTALAGKELPEAFSGHKT
                     ASTSTYTEVLTEH"
BASE COUNT         1738 a         1397 c         1210 g         1820 t
ORIGIN      
        1 cgcattagac aaacctaccc tactagaggt cgagatccaa aacatgatgc cgcccttcca
       61 aatagtagaa tagttaatct atatcaagtt caatccgggt cataagaagc taggtcctca
      121 aacgggtata gattgcttcg tctcattcga tattcaaaga agttcaaatc cgaagttgta
      181 acacctgggg tgttaactaa aagaatttcc tttgctatcg gctggtatgc cggccttggc
      241 gagactacgc ctttggccac tacaaccctg tatttttctg gataaactcc gacagaatac
      301 atttgctccc gacttgtatt tccacatctc agagacgtca aaaccaaggt atggtcatcg
      361 tcagtgtcaa gtactgccgt taggccaccg tcataatact tgaatcctcc gtgagtgggg
      421 cggtcgtcct caaatttccc attaaaaagg gtcctaacta ttccagtaac acttacgggc
      481 aacccgtgca tatcatcggt tttagcacct acatccacag tcacttcgga acccaccccg
      541 gcactaacac atgccttcac tgattttggg tcccacaaag tctggaggta ccctttaata
      601 cccatacttt ttgcaagact caatatgtga gtggaatcag cactactgcc cgccccgata
      661 ttatctccga catccattag gacatatgga ccatcttcct gatttatgta ttttttgtct
      721 gcgtatgaca aagcatcttc aattgaaggg gtatctccga caaactggtc cctgatattc
      781 caggcttccc tagccatcca ttgagaagcc tccttggcag cttctatgtc tccatcagat
      841 acagaaataa accccattcc catttcttct acgtcagcat atgggtaacc ttcagcaaca
      901 cttccatgaa ttatgtttgg aaaggaaaga gtcttatcga gtttttccat aacggatttc
      961 atgggatctt catcggtaaa ttgcttaact atattaatta caacaggggg gatttctaac
     1021 cacaggaccg ggtcaatctt tcctttgata gaatctcgta tcatttcggc gcattcaaat
     1081 gcccttattt tcggatctaa atgtggattt gtcctataaa cagtcgcaac gtcaatattt
     1141 tcaatcattt tccgagaaag attagcatgc atatctacac tcaatccaac cttcacgtcg
     1201 gggcctatca aagttctaac cctttcagcg atttcaccat cggcatcagg atagtgttcg
     1261 gacactgctg ctccgtgcaa tgatagtaat actccatccc agggtccatg ggtctccagt
     1321 aaggaaagca tttccttact tatagattcg aaggcatcgg cggttattgt gccaatcggg
     1381 cctgtaatcg caaatatgag aggcactacc tccacatctt tatcctctcc tatctgtaaa
     1441 aatccggcgt tagtagtttg ggatgtttga tattctcggg ttatctcctc ctttctatag
     1501 atgttgaagg cagcatagtc ggcaaccact tttgaaaaag tgtttgtttc atgataaatc
     1561 cccaacaatg caagcttcat ctttgagcac cttcaatttc ttgtttatta agtttgtgta
     1621 ttataggtct ttgtagttgg atataagtca ccgcagctac cagaggcaat ttaaggagag
     1681 acctatatat ggaccgggaa cattcatatg atatcagtgg tgagctatac aacagagctc
     1741 ttaattctct cgcgggtggg gttaattcca atgtacgctt gaccgagaca cctcatcctc
     1801 tgttttacga caaggccaaa ggcagtaaaa tttatgatgt tgatggaaac caatacatcg
     1861 attacgtcct agggcaaggg cctatgattt ttggccactc acctgatttc ctcctacagg
     1921 cggtagcaga agcatcagag tcagggcaac ttttcgcagg ccaacattta ctggaattag
     1981 aagctgctga ggcagtgaaa agcctggttc caaatgctga actggtccga tttgcaagtt
     2041 caggaacaga ggcggttgag gctgcctttc gtctcgcaag ggcatttaca cggaggaacg
     2101 taataataaa atttgaaggc caatatcacg gctggtcaga tggtgtgtta ttcaatacag
     2161 cggctcctct cactcctctt gatagcaata tctttcccga ggttgaacct atgagcgaag
     2221 gaatcgtcga tcattctgaa tccgctctga taattttacc ctggaacaac tttgaagcga
     2281 tagatgcagt ctttgaacaa aggggtaacg aaatcgcagc ggttattaca gaaccaatca
     2341 tgtgcaacac aaattgcata atgccaaaac ctggttacct gcagcactta aaaaaatctt
     2401 gcgaacgtaa tgactcctta cttatatttg atgaggttat aacaggattt agggtcgatg
     2461 caggtggagc tcaatccctt tttggtatca ccccacattt ggccactttt gctaaagctg
     2521 ctgcaggagg attccccgtg tctatactca ctgggagtaa agaaataatg tccttaattg
     2581 gtacaggtac agtcatgcac ggcggaacat taaatgcaaa cgtaatgtct atggctgcga
     2641 ctaaatcatc gatggaacat ttgtcagaca gggcaataca aacgaatcaa aaattacaag
     2701 atcttggaaa aagactaatg gaagggcttg aaggcatcaa ttccagattg gaagcaggaa
     2761 tgctgattca ggggcccggt tcagtcttcg ccgtgagttt caccgatggc agagaaatat
     2821 ctgactatag gagccatgtc gaaaattcag atcccgacaa atacgcccta tttgtatcag
     2881 agatgatgaa aaaaggtatc aggataaatt cacgaggaat ttggtttctt tcagaatctc
     2941 ataccgatga ggatattgaa gctactctat ccgctgcctc ggaagttttg caattgatta
     3001 agttgtgagt tagttataca gccctttcaa atatctgaac tctatgacga ttactctctg
     3061 tcacgtatac cctgccttca tcgtctaatt ttaccgaggt agggccccaa aatagctttt
     3121 caatatgagc agattcttca tgtgggtctc ccccaaattg aacagggttt ggttccaaat
     3181 tcgatctgga tctaggctct gcttcttcat aattaccttc gaagtattca tccgcccatt
     3241 tcgagatcgt ggcttggcct ctaatggttt gtatgaaatt accgttcttg tccaggatct
     3301 caagcctttc attaccccag tcagttacat aaatataacc atccttatct acagcgacac
     3361 ctgatgggtt tttgaactgc ccgtcttctg aacctcggtt tccaaaggat ctaatgaacc
     3421 ctccactaga atcatatttg gctatgcagt cattccccca atcggccaca tacacaaaac
     3481 catcactact aacaaaaacc ccccatggca tattaagagt atttcgacct ttgtttccaa
     3541 agctcaaaat aaatttgcca tcttttgtaa atttttgaat tctattattc atatgatccg
     3601 atacgaaaag attaccctca gaatcgaacg caattccaga aggtccgttt aattcgcctt
     3661 caccggaacc agcttcaccc caatatttaa tggggtcacc ttcatgtgaa aagacgttta
     3721 tacggttatt caattcgtca ctgacgtaga tattgcccat agaatctgat gccataccgg
     3781 tgggccacct aaattggccg agactttctc cgtagccacc gtaaataccg aaatattcac
     3841 tttctatgtc atacatagtt atacgtaact gggcagcagc aggagcatat gccctgctga
     3901 cagtatgcat ccttccgttt ttcccaatag cagtatctac aggaaacatg aatcctctgc
     3961 catccatgac acatattcct atggtatggc tatattttag aaatgatttt tcagtctgcg
     4021 ttgtagtcac caaaaatact ccacgaacat caagattgtt ggaattctgg aacagatgcg
     4081 acaacgctca ttaattcgga aatctttttt gctgcattct ctttgtcttt gactagatta
     4141 atgcttggaa tcccgttttc ttgagcaaat tcaactaagg tatcgtatat atattgggag
     4201 gtttgaatcg ctcccaattg atctaaacat ttatcaacgc acactgaagc ggatatacac
     4261 tccccctcat catccatgac attttgaaca attcttctca ctccaggttt ggataggtca
     4321 ccgagatgct gcgaggcaaa attgacacgc tccgtcaaag aacctgtttc aatccattca
     4381 aggccttggt gccacccttc aacagatgga ggattcagca gttgctgtcc cataaatgtg
     4441 atctgactat tacgttctga aatacgaata ttaggtgtcc caaattcctc agttgtcctc
     4501 aagacattac cagctaattc agcaggactt ttgatttttt tgtaatgaca tgtatcagac
     4561 ttgaaaaagt cagaattgaa aagtacccgg atcatttctc caatatcata cccactgtca
     4621 aaatatgcct tttctaaaat agagatcgct gccaagtcct ggggttcctt atacggccaa
     4681 gatggtacag gtggttcatc ggcgacaaaa aaatgatata ggtgtctaga gatgaatcgg
     4741 gcggtggcgg gttgtgcaca gattatatct ataatctctt cgccattaaa atttcctttg
     4801 tgtcccaaaa aggttttttc tccctcgtca tgatcttctt ctttgtattc atagcgccat
     4861 gccaatttac cgtagggcat tattgaattt cgaacggcca actgttttat ataatctgaa
     4921 ttggcaatac tccaacctgt aaaggctctc gaacattctt taatatcgtc ttcattgtag
     4981 ttaccgactc ccatagaaaa cagttccaga agttctctac cgtaattctc gttaatggca
     5041 ccattatgat tgtctacatt atccaaccaa ataatcattg cggggtccct cgataattta
     5101 agcaacagtt cgtctagttt ggccaggccg tggctgcgaa acatccgtaa ttgatcactc
     5161 attggttttc cgttagttac ttttgtgtac ccagtcgcaa aaacgttgtg ccaaaaaaga
     5221 gcgattttct ctctcaacgg ggaatctgtt gataccatcc tatacaacca ataagatccc
     5281 gccccagttg tatcgtgtgt cacagattga tcaggaaaat atctcctgac caacgcgtta
     5341 tcgatccggg atgaatcctc attatcgatc aaagaatcta cgaccctgtc atatcctatt
     5401 gaaatatatt ggtcgagttg ttcttttgta gcaccaaacc cggctcttct aagtaaatga
     5461 gccatcaatt ttacatcagt actcgcgctt accattggtt tcactcccct cagccatcca
     5521 agtttcaagt aatttatcat aaatcttggt tgtattcata actatcatag gttttttcat
     5581 ctgttcttat gtaatataga gttttcgcta aattatcgga ataaatgatc catctgggag
     5641 ctttgattgc cttttgcaaa attgcccgat tccaacattc atttcgtacg caaaggttca
     5701 ggggagataa cttacgtatt ttgccatggc cttggtggaa gtggatcaag atttgaaaag
     5761 gaagacatcg attggtatgc ggaacatttc gatgtgattt cttgggataa tcgaggacta
     5821 ggtagatcag gggcatctga aaaatattca ctacctcttt atgcaatgga tctgaaaaat
     5881 ttactccact atttgaaaat tgaaaaagcc gttatatttg gggtgtcatg gggaggcgtg
     5941 ctagctcaga ggttcgccac aatgtttcct gaaacatgct tagccttagt attggattcc
     6001 acctcgagcg aggtaaacgt taaggcatca gaaaattggt atgctagggg agaaatagcc
     6061 aggttaggag aacaaacagc cttagcagga aaggaattac cagaggcctt cagtggtcat
     6121 aaaacggctt ctacaagcac atacactgaa gtcttaactg aacac
//
LOCUS       DASCNS010000018        23588 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000173507, whole genome shotgun sequence.
ACCESSION   DASCNS010000018 DASCNS010000000
VERSION     DASCNS010000018.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 23588)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23588)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23588
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000173507"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="coxB"
                     /locus_tag="QF838_00580"
     CDS             <1..1076
                     /gene="coxB"
                     /locus_tag="QF838_00580"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HJO59223.1"
                     /translation="FLLAATLLTLLITACYPDHNQSTFDPQGPVASRQLGIFWWIFIG
                     GMIVFVLVEAALIYAMIKFRARSEFDNPAQTHGNNRLEIIWTAIPAILLLIIMVPTID
                     TLFYMYEAPKLHQPNHTLEVIGHQWWFEFRYPDPNNPEKEIVTANEVYIPVGYPVPIN
                     LGSVDVIHSFWVPKLAGKTDMVPGEENYMWFEADVPGEYYGQCAEYCGTSHANMKFKV
                     IAVPENEFNAWLQTQAASAFESSDALVQEGAGVFKSAGCTGCHATKTVVNKGSTGRVG
                     PNLAHVASRRDLAAGMLSNANENGIVNDALLQENLRTWLQDPNEVKPGNLMSSGAQVY
                     TDPDKKLSEEQISQLVHYLSSLK"
     gene            1114..2970
                     /gene="ctaD"
                     /locus_tag="QF838_00585"
     CDS             1114..2970
                     /gene="ctaD"
                     /locus_tag="QF838_00585"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HJO59224.1"
                     /translation="MSTFPISIPRPVHPTGIWNWVTTIDHKRIGILYGVTAFLMFLSG
                     GVEAILMRVQLAGPELDIVSAAVYNQLFTMHGTTMIFLAVMPLSAAFFNFVIPLQIGA
                     RDVAFPRLNAFSYWTFLAGALMLKLSWIGGATDAGWFGYAPLTSVAQNPGTGIDFWII
                     SLQILGVASLAAAFNFIVTIINMRAPGMTMMRLPLFTWMSFITAILLVLAFPVITVAL
                     IELTFDRYFMFNFFNVANGGSVVLWQHLFWVFGHPEVYILILPAMGIVSEVIPVFSKK
                     PLFGYKTVVLAGAIIAFMGWMVWSHHMFTVGLGAVANAVFTVTTMLIAVPTGIKVFNW
                     LGTIWGGSIRFTTPMLYSLGFIAMFLIGGISGVMHAVSASDAQQQDTYFIPAHLHYVL
                     FGGAIMAILSGIFYWFPKYSGKMFNERQGKISFWLLMIGQNVTFFPMHFVGLDGMPRR
                     IYTYAGNMGWDFWNGVATGGVLILLIGFLLVMDNIGRHWKNGEPAPPDPWDGRTLEWS
                     IPSPPPEYNFEEIPIVRGLDDWWATKQGDSYKEIPASGGSGENGHNIHLPQPSYWPFV
                     SAAGLFIAAYGVVFNDLLIPWGLAAIGLTIGFVGVYAWSLEPVNDPEEDSTH"
     gene            2984..3709
                     /locus_tag="QF838_00590"
     CDS             2984..3709
                     /locus_tag="QF838_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HJO59225.1"
                     /translation="MAQEHAAPIHGEDEGTTTGLNHRKMLMWAFLGSDCMFFGSLIAT
                     YLVYQGKSLEGPLPIDVFDIPVTSISTFVLLMSSMSMVLAYSALTKNNMKGFRIWMIS
                     TAIMGSTFIGFQVYEFSSFATHHVEIDCVNPGELTKYEQHIFDDGCSSGEAHAESHEG
                     LKPQTNLFGTTFYTLTGFHGAHVTLGVVWLLSLLLLSFKKGVITPEKNLDVDLAALYW
                     HFVDVVWIVIFTVVYLFGVFPGF"
     gene            3729..4034
                     /locus_tag="QF838_00595"
     CDS             3729..4034
                     /locus_tag="QF838_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HJO59226.1"
                     /translation="MSQELSAEEHTGHPTAGQYFRIAVILCVITAVEVGVFYVEFLGH
                     WMIPILFILSASKFTLVAMYYMHLKFDHKLFTYLFVAGFILAALIISFLLLLFGVYS"
     gene            4916..5119
                     /locus_tag="QF838_00600"
     CDS             4916..5119
                     /locus_tag="QF838_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59227.1"
                     /translation="MNKVTLALVVPLASFAMIAVFAITLGFTFYQIHHNTGLGTIGVI
                     VIGLALLILTPLVAFLLEKKTSP"
     gene            5248..5772
                     /locus_tag="QF838_00605"
     CDS             5248..5772
                     /locus_tag="QF838_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014667.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HJO59228.1"
                     /translation="MYGTALNDHDGSRFSLLDENAVEVTDQTYMGQVHLITFLFTNCT
                     SLCPLVTASIKQSLERSEDTRNTPVLIISVDPKGDTIESRKAFKNRWGLSSNWRYLNG
                     NKEELNTIWKSFYLNPQEAAIESLNSQMNRKYDIIHSSPVYIVDDEGRPAVVHTNPID
                     PDHLHEDLIRISKR"
     gene            complement(5796..6158)
                     /gene="rplT"
                     /locus_tag="QF838_00610"
     CDS             complement(5796..6158)
                     /gene="rplT"
                     /locus_tag="QF838_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HJO59229.1"
                     /translation="MTRVRTGTTRRRRHKKILNMTKGHQGVRHTLYRRAHESMIHALQ
                     YSYDHRKKKKGDMRRLWNIRINAAARSNEITYSKLIHGLKLAGIDINRKMLAEMAISN
                     PESFTGVVESAKTALEAA"
     gene            complement(6186..6386)
                     /gene="rpmI"
                     /locus_tag="QF838_00615"
     CDS             complement(6186..6386)
                     /gene="rpmI"
                     /locus_tag="QF838_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HJO59230.1"
                     /translation="MPKLKTHKGAKRRFKVTGSGKLMRMKGLRSHLRRKKPKSVKRLY
                     SGTLPVSESDVPRLKKLLPYSK"
     gene            complement(6373..6957)
                     /gene="infC"
                     /locus_tag="QF838_00620"
     CDS             complement(6373..6957)
                     /gene="infC"
                     /locus_tag="QF838_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HJO59231.1"
                     /translation="MKRGSNIPKNYRVNDQITAQTVLVVDNKGNQLGELTTDKALEMA
                     TEKGLDLVEVAPSANPPVCRLLNYGKFRYEATRKEREARKASKTKSNNSVREVRMKTR
                     IGDHDREAKTRLVKRLLTEGSKVRVSVMFRGREVQHPQIGMALLKNVAEDLQEDALLE
                     KAPSFEGKFLAMILAPSPSLKKNKNNREPESAKT"
     gene            complement(7045..8781)
                     /gene="thrS"
                     /locus_tag="QF838_00625"
     CDS             complement(7045..8781)
                     /gene="thrS"
                     /locus_tag="QF838_00625"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HJO59232.1"
                     /translation="MNSEELKNLREKIRHSTAHVMADVVTQLYPEAKLAIGPPTEDGF
                     YYDFMVETPFTDEDLKKIEVAMGKVISEDLPFIYAEYTREQISVMNQSEPFKLEVISD
                     IPEQEVISTYSHGEFEDLCAGPHVESTGKIPAFKLLTVAGAYWRGDENRQMLQRIYGT
                     AFESQDTLDEHLSRLEEAQRRDHRTLGRELDLFSIHEETGPGLIIWHPKGARVRSIFE
                     DFWRKEHYSRGYDLIYTPNIGRENLWQTSGHLDFFQENMFPPAETDGQNYYLKPMNCP
                     FHIMYYKSSLRSYRDLPMKIGELGTVYRYERGGTLHGMLRVRGFTQDDAHIFCTPDQI
                     ETEIDGVLDLTFDLMEAFGFDDFTLNLSTKPDKAVGSDEQWQLAEKSLMETLDSRGLD
                     YQIEEAGGAFYGPKIDVNIRDAIGRSWQCTTVQFDFNLPERFGLTYIGEDGGPHQPFM
                     VHRAIFGSLERFMGILIEHYGGAFPTWLAPTQVQIIPIADRHFDYATFLHSELSESGI
                     RSAIDSRSERMNAKVRDAQIAKVPYMFIVGDREVESESVGVRLRSGEDLGALSLNEIK
                     KRISSEVEDKIT"
     gene            complement(8804..9544)
                     /locus_tag="QF838_00630"
     CDS             complement(8804..9544)
                     /locus_tag="QF838_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014035.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HJO59233.1"
                     /translation="MDSLVVDILLTILAIFVGSLVLVTAGFGIAIGSSPIMLLTLDPK
                     STVIIINTVSLLIFVLMIHQNRRHINYREMTVPVIFGMMGVPVGVYILSNSNPSALRV
                     SIALLIIFLGFVMVLNPTIAAIRHRYTLWGAAFLVSAMITSTGIGGPIMAIAALSREW
                     DRESIRGSLPYYYLFIETTAVIGYFITGMFDSERLILTGVSIFPALLGFFIASMLVKK
                     INQSYYRRLILGIVICAGTVILVKEVFV"
     gene            complement(9550..12294)
                     /locus_tag="QF838_00635"
     CDS             complement(9550..12294)
                     /locus_tag="QF838_00635"
                     /inference="COORDINATES: protein motif:HMM:NF019311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HJO59234.1"
                     /translation="MSILSGLAFIFVSVKLWDHGFYLISLPTFFVGIIAILISANKYA
                     IPISRRDNCPRSTGVSLGSKSSKVLWLKLTEKAAGWFLVMLSAILSISGFMLFDRAAH
                     GAGWACYMFSMLALLAVPFLFRGSAVPVFKPFINSRLTIPVFTTFLITGVVILGFVLR
                     VYNISELPAGLWYDEADNIIRAGQIHASPSNTPVFVPSTHLPSAFLVPIAMLQELTSV
                     SWLNGRLISAGFSVILILAMFYFAKDVFGAFWGLGGAFGIAVMRWSLNWGRIGMHGIT
                     AAVFSALTGLLLWRSLINWSPFWFFWTGMALGVGMWFYAPFRLFPIVILIGVGLRICS
                     GFPGWRRLVDCLVSMALAAIISTVPLLQYSFRNPEIFFKRTEETFILNHLSDVSWFGA
                     IWGNFVEHIFMFHISGDPNPRHNLPFEPMLDDVTGTLFIAGIILIVAQWRRPLFLLFP
                     CWVFVMLTPGIFSVPWESPQSLRSIGVIPAVLIISIVPLVHLLCLFNLNPRDIFRKGG
                     LVFIIVLFGFIGYFNVSMYFGKQASHPDVFADFSTSETLMAKEMVKQSQNGYTLYSSR
                     QFLFSLTASVVSGNVHYEPLFAPRDLPISPNRVLHGAAIYLEPRDAGIYDLLAEYYPS
                     AKFREIMAPHGVDPILYEVLINKQQLVDSLGVEATFKREDVLVKTDKFDIFSYSWAKD
                     LSGVAFPVDFVVQSNLHVREQGLYQFQVSGDGQLYIDGISIQENGDGIKLGVGLHSLR
                     LKGIAHSEAGFVEVLWGRDGNIMEPIPSALLFLGDRSPTGLAAVFYQESQPVVSHVGL
                     TADTFYYAPPIEGPYEAVWAGFVEIPISGEYTFSLTSNSAMKLIVNDVLLAETAWDSE
                     FARSGEISIHGGKTSIELRSLPSTRSPQFKIFWQMKGNDEDVIPVSLIKPDPEFMRIP
                     "
     gene            complement(12346..14634)
                     /locus_tag="QF838_00640"
     CDS             complement(12346..14634)
                     /locus_tag="QF838_00640"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59235.1"
                     /translation="MEVFDGINVIDTTSGKAGAICSMFLSDNGARVLRVPAKQKINRD
                     HPEFATLDRGKELHGLDLNTQYELFEKLVQKCDVIIEDLLPLDTLQKKIGFQTLGKIN
                     SRLLHCSITPYGQKGLLKDHSPISDLIKARTGILDSMPGFEEGSTHVAHPIIDVGAGL
                     LAALGLASGLLYRLKSGAGLKINTSLMAAGLLYMPKAIGDRVRPRPVKVTPVGGGPFY
                     SVYECQDSAWIQLGCIHAGFVDIAATVMGIAEVMINPRYGDGRNPVDEEARSELFDIV
                     KNVMKTRPSHEWAELFESVDVPFAHAATADAAIENQQVVHNQMVQELIDPIYGPMIQH
                     GLPIKFSNTPGSVKGPRVLNSTSGTILKTSLQTENNLENHSVDKLPLNGIKVADITNV
                     IAGPTMGRLLADLGADVLKIEPPYGDISRPSSGRYFHALNANKRSISIDAKNEVAQGV
                     LQNIVGSCDVMVANLRPGATGRMGLDTETLRKFNPKMIEVHVTAFGWDGPFAKRPGVD
                     PLAQAWMGLQVAQGGEGNPPSFLAPVAPTDYTAGCMGALGAVMALYAKEKNGIGQVVN
                     TNLLNAGCLLLEGDFSRYKNKIQRRISDKSQNGLSDFHRLYQTSDGWIYVSAEDSVSG
                     DSLLDVVGLTNRDWNSSRREHSELGSQVEEAIKLHSSRYWLELMLSNGIPCAESITAY
                     EAGFFKDEQAIRNDMISKSVLDDGSEYAFSYNLIQFPDLPIPQVKPTPLLGEHSLEIL
                     KEFQIDESVIDTIFQNGSLVTP"
     gene            complement(14752..14964)
                     /locus_tag="QF838_00645"
     CDS             complement(14752..14964)
                     /locus_tag="QF838_00645"
                     /inference="COORDINATES: protein motif:HMM:NF021758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HJO59236.1"
                     /translation="MAAWMANEFFAEAGKDIAIRITPGTGGIYQVFADGEKIFDKAEE
                     DGLFPNLPRVKELRAIIKEKLATPVA"
     gene            complement(15139..16431)
                     /locus_tag="QF838_00650"
     CDS             complement(15139..16431)
                     /locus_tag="QF838_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024877.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HJO59237.1"
                     /translation="MAKRKVGKIIKRVGLKKGDPDIEIPIAADLLKVDGVPQRDTEVS
                     YYSREFPLESFSIEQSASAVWAQKERAEHTPETEELYKNYQKKITPWIDKIKRSGELA
                     PNVSSQTENSTELIRSKAKELGYSEIGFTKFDRRYIYQSRKTYVRNNLPNVICLAYEQ
                     EYIETQNIPSYSSEIAQGDAYLTQADLSLKLADFINELGFSAQISGPAWHFGPMIPMF
                     VEAGLGQLGVNGQLLSPHFGSRARLQLIITDAHLDYDSPIDYGMYKFCELCQVCFMRC
                     PGKAIQAQRVWFRGVEKNKLIAKRCRPIMARYSGCGICMKVCPIQKYGMQPVMEHYVE
                     TGEVLGKGTDDLESYELENKGYFRSGKVPVFDRDFFDMPEGRTEHNLVEEFKDNLMNM
                     KNSDKQSEYELYLEFKSALDSSKTRKGEVVDMGMDMEF"
     gene            16571..16897
                     /locus_tag="QF838_00655"
     CDS             16571..16897
                     /locus_tag="QF838_00655"
                     /inference="COORDINATES: protein motif:HMM:NF017922.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HJO59238.1"
                     /translation="MTQGLKIEKIGVYGDKILIRWTDGHESTYGAKEIRISCQCAECV
                     EEWSKRQLLDPATVPSDLIVEDHLMVGNYAIQFLWSDAHYTGIFPFDTLRKMCPCNSC
                     ENVPLQ"
     gene            complement(17014..17424)
                     /locus_tag="QF838_00660"
     CDS             complement(17014..17424)
                     /locus_tag="QF838_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HJO59239.1"
                     /translation="MAYNKPLPVPQGESDVYWQKAKQGELWLRNCKSCGNAYFYPRDI
                     SPCCFSKDTDWIQASGKATLFTYGIVQRAPHPGFVDDVPFVTAVVELEEGPKMATNIV
                     IEDPTPENLQIGMALEVVFEDITDELALPKFKPA"
     gene            complement(17441..18583)
                     /locus_tag="QF838_00665"
     CDS             complement(17441..18583)
                     /locus_tag="QF838_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HJO59240.1"
                     /translation="MTDLRKTVAIVGVDESDEIGKVPDKSNLTHHAEAAYNALEDAGM
                     SMKDVNGLFTAGTSTLNLAEYLGIEPSFTDTTAVGGSSFIIHIAHAMAAINAGYCDVA
                     LVTHGEAGRSRRSRPGGDAADPGSQFETPYGFLGAPINYAMAARRYMHEYGEDKTRQA
                     MAEVAVATRKWAQLNPKAYMKDDMSFDDYHNSRWISWPFHLLDCCLVTDAGGAYVITS
                     AERARDAKKKPVWVLGAAESHDHNLISQMPDLTRTVAKNTGPLALERSGVTHDDIDLA
                     MIYDSFTYTVLATLESLGFCGPGEAPDFVANQRTAPGGDFAMNTSGGGLSYTHSGMYG
                     MFLVLEAVRQLRGECGDRQLDDPKLCLINGTGGSLSSTGTTVLAVD"
     gene            complement(18714..19880)
                     /locus_tag="QF838_00670"
     CDS             complement(18714..19880)
                     /locus_tag="QF838_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO59241.1"
                     /translation="MKVTQIKSFPIADEDGRVYYIVKVETDSGIYGLGEVGIRNWGGA
                     IGKAIEHLSEVVIGEDPFSTEKMWQQMFRGGFFPADKVYSCAISAIDIGLWDIKAKAL
                     GMPLYKLLGGPVRDKVICYPHTQGDTMTELLENCKRHIDAGWKFLRWHQPETGPSAAY
                     VDNLNTLEPVESIRIAEQQIATIREAVGPDIQICFDMHTRLDTAHAVAFCKAVEPYRP
                     FFIEDPLRSENTSSYRTLARHTSLPIAAGEQWASKWPFREVIEEELINYARIDLCIVG
                     GITEALKITHWAETHYIDIVPHNPLGPVSAAACVALCMASTNVGVQEMPRRPGSYATD
                     LFPKQIEWEDGYAWCPDEPGMGVDFNEELALDRIVNPTGWPPQLRRNDGSFTNW"
     gene            20016..20357
                     /locus_tag="QF838_00675"
     CDS             20016..20357
                     /locus_tag="QF838_00675"
                     /inference="COORDINATES: protein motif:HMM:NF018021.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HJO59242.1"
                     /translation="MISLSERKFQSALDRAMADLSDALKKCLKEIDIIIYEIAEKSDL
                     LGTNVEKELDLLSLYEIVPISDGYHNERNLRGQLTLFKRALEAICEEESDLVPQLTTI
                     LEEELSYRPSL"
     gene            complement(20397..22256)
                     /gene="selB"
                     /locus_tag="QF838_00680"
     CDS             complement(20397..22256)
                     /gene="selB"
                     /locus_tag="QF838_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HJO59243.1"
                     /translation="MYVIGTAGHVDHGKSTLVESLTGIDPDRFQEEKDRGMTIDIGFA
                     WITLPSGREVSIVDVPGHEKFVSNMLAGVGGVDMALLVVAADESVMPQTREHLAILDL
                     LGVTNGVVVVTKEDLVDKDWLELVYGEVEELLAGTTLEGAEIISASATTGSGISDLMH
                     FIDLALDKTTAKKDFNKPRLPIDRSFSISGFGTVVTGTLIDGFLSVGQEIEIIPIGLK
                     ARIRGLQSHGKSETSFGPGTRVAANLSGIESTQITRGDVLSPPNFIDLSEAFDVRLNV
                     VEDAPNPLRHNMFVTLHTGSSEVVARLRLLEGEEVQPGNSTWAQLKPEKSLPVLRGDH
                     YIIRSNMTTLGGGSIVDTQAKRHRRRHSPTIQKLESLESDIPSQIILNMIESSSPATL
                     SSLLSGSKLQEEEIIPTIDFLIAEKTVMSTSLDVKKSYFFTVKRWEEICDLTKQSLNL
                     FHVQFPLRQGMPREELRNKMTLSSESFNAVVDVLEEIGLLRNFTSIIAFTGHKSHLTE
                     EQKRVSEAYVENIAKDKFSPRTDVEIDEDILSFLVDKGRLVRVSDGIVFSSEAFKEMS
                     DGVRAYISQHGQITVGDFRDLFQTSRKYALGFMDYMDQQQITRRVGDARILRE"
     gene            22398..23585
                     /locus_tag="QF838_00685"
     CDS             22398..23585
                     /locus_tag="QF838_00685"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HJO59244.1"
                     /translation="MNDAVIVSGARTPVGRFGGAFKDIGAPDLGSTAIKAALDRAGVS
                     PDMVDEVVLGNAIQAAEAGYAARLASLQAGIPHDTPTIAVNRQCSSGLEAINMAAQLV
                     RTGEADIVVAGGTENMSQVPYLIDYKARFDGLRMGNSQLIDGLTEGLGCPVNRYHMGV
                     TAENVAERFSVSRKDQDELAVMSHQRASTAISEGKFTNQIVTVSVPQRRGDPIVVMED
                     EGPRADTTIEGLSNLRPVFKKDGTVTAGNASSINDGSAAVVMMSAQKAADLGIKPRMK
                     WHTRGIAGVDPAVMGIGPVPAVQKAINKSGMVVNDIDLIELNEAFASQALYCIRELNL
                     DIEKTNVNGSGISLGHPVGATGAIMTVKLMEEMERSDSTTGLVTMCVGGGQGVATIFE
                     RLN"
BASE COUNT         6748 a         5471 c         4621 g         6748 t
ORIGIN      
        1 cttttctctt agctgcaacc cttttaacct tattaattac agcatgctat cccgaccata
       61 accagtcgac cttcgaccct caaggacccg ttgcaagccg gcaattaggt attttttggt
      121 ggatatttat cgggggcatg atagttttcg tcctagtgga agccgccctt atctacgcaa
      181 tgattaaatt ccgggcaagg agtgagttcg acaacccagc tcagacgcac ggcaacaaca
      241 gattagaaat tatatggaca gctatccctg ccatactctt attaatcatc atggtgccaa
      301 ccatcgacac ccttttctat atgtatgaag ctccgaagct ccatcaacct aatcacaccc
      361 ttgaagttat aggacatcaa tggtggtttg aattcagata cccggatccg aataacccag
      421 agaaagaaat agttactgca aatgaagtct atatacctgt gggatatcct gtgccaataa
      481 atctgggttc cgtcgacgtg attcatagct tctgggtacc taagctggca ggtaaaactg
      541 acatggttcc aggtgaagag aattacatgt ggtttgaggc tgacgtgccc ggagaatatt
      601 acggtcaatg cgccgagtac tgcggaacat cacacgccaa catgaaattc aaggtcatcg
      661 cagttccgga aaatgaattt aatgcatggc tgcagacaca ggctgcctcg gcatttgaat
      721 catctgacgc actagtgcaa gaaggtgctg gggtttttaa gagtgcagga tgcaccggat
      781 gccacgctac aaagactgtt gtaaacaaag gttccacagg aagggtgggc cccaacctgg
      841 cccatgtggc ttctcgcaga gatcttgcag cgggtatgtt gagcaatgcc aacgaaaatg
      901 gaattgttaa cgatgctcta cttcaagaaa acctgcgaac ttggttgcaa gacccaaatg
      961 aagtgaaacc agggaactta atgtctagtg gcgctcaggt atacaccgat cccgacaaga
     1021 aattaagtga agagcagata tcacaattgg tacattatct gtcttcattg aagtagtttt
     1081 ggaattatca cgaatcgagc tggaggccgc taagtgagta catttcctat atcaataccg
     1141 aggccggttc atccgactgg catatggaat tgggttacga cgatcgatca taaacgaatc
     1201 ggtatcttgt atggagtaac cgctttcctc atgttcctct ctggtggcgt agaggcgatt
     1261 ttaatgaggg tgcaattagc cggccctgaa ctggatatag tgagtgcagc tgtctataac
     1321 cagctgttca caatgcacgg tactacaatg atattcctcg ccgttatgcc actctccgca
     1381 gccttcttta acttcgttat accgctgcaa ataggagccc gtgatgtcgc cttccctagg
     1441 ctaaatgctt ttagttattg gactttcctt gcgggagcct tgatgttgaa gcttagttgg
     1501 attggtggtg ctacagatgc gggttggttt ggatatgcac ctctcacctc cgtggcccaa
     1561 aacccaggta ctggaataga cttttggata ataagcttac agatcctcgg agttgcgtca
     1621 ttggccgctg catttaactt tattgtgacg ataattaaca tgagagctcc tgggatgacg
     1681 atgatgcgcc ttcctttatt tacctggatg agcttcatca ctgcaattct tttggtgtta
     1741 gctttcccag ttatcactgt tgcattaatt gaacttacat ttgacaggta tttcatgttc
     1801 aatttcttta atgtggccaa cggagggagt gttgtgttgt ggcaacatct tttctgggta
     1861 tttgggcatc cggaagtgta cattcttatc ctgccagcga tgggtattgt ttctgaagtg
     1921 atacctgtat tctcaaaaaa acctttgttt ggctacaaaa cagtagtttt agcaggagca
     1981 atcatagcgt tcatgggatg gatggtatgg agccatcata tgtttacagt tggactagga
     2041 gctgttgcta atgccgtttt tactgttacg accatgctga ttgcagtacc cactgggatc
     2101 aaagtgttta attggcttgg aacaatttgg ggaggatcca ttagattcac cactccgatg
     2161 ctatactcgc taggttttat cgcgatgttc ctcattggag gaattagtgg agtgatgcat
     2221 gccgtgtcgg cttcagatgc acagcaacag gatacttatt tcatacctgc ccacctgcat
     2281 tacgtgttgt ttggcggggc aataatggcc atcctgtccg gcatcttcta ctggtttccc
     2341 aaatattcgg ggaaaatgtt caacgaacga caagggaaaa tctctttctg gctgctcatg
     2401 attggccaga acgtgacctt cttcccaatg catttcgtcg gattagatgg gatgccgcga
     2461 agaatttata cctatgctgg caacatggga tgggattttt ggaacggtgt ggccaccgga
     2521 ggagtgctta ttctcctaat cgggttcctt ttggtaatgg ataatattgg aaggcactgg
     2581 aaaaacggcg aaccggctcc accggacccc tgggatggta gaactttaga atggtcaatc
     2641 ccatcaccac ctccggaata taacttcgaa gaaatcccga ttgttagagg gctcgatgat
     2701 tggtgggcaa ctaaacaggg agattcttat aaagagattc cggccagtgg aggatcggga
     2761 gaaaacggcc ataatattca cctgccccaa ccttcatatt ggccattcgt ttcggccgcc
     2821 gggctattta tagcagccta tggagttgta ttcaacgatt tgctaatccc gtggggacta
     2881 gcagcaattg gtctgacaat aggatttgtt ggtgtttacg catggtctct tgaaccggta
     2941 aatgatcctg aagaagactc tactcattaa ggaaggttct caattggcac aggaacacgc
     3001 agctccaatt cacggggaag atgaaggtac cacaaccggt cttaatcacc gaaaaatgct
     3061 catgtgggca tttcttggct cagactgcat gttttttggt tccctgatcg caacatattt
     3121 ggtataccaa ggcaagagtc tagagggccc actaccaata gatgtgtttg atataccagt
     3181 tacctccatt agtaccttcg tacttttaat gagtagtatg agtatggtac ttgcctactc
     3241 tgccttaaca aaaaacaaca tgaagggttt caggatatgg atgatatcca cagcaataat
     3301 gggctcaacc tttattggtt ttcaggttta tgaattcagc agttttgcga cccaccatgt
     3361 agagatcgat tgtgtcaatc caggagaatt gaccaaatac gaacaacata tttttgatga
     3421 cggttgttct tccggtgaag cccatgcaga aagtcatgaa gggttaaaac ctcaaacaaa
     3481 tttatttgga acaacttttt acaccctaac gggttttcat ggtgctcacg ttactttggg
     3541 agtagtctgg ctcctttcgt tattactttt atcatttaag aagggtgtca taaccccaga
     3601 gaagaatttg gacgtggatc tggctgcact ctattggcac tttgtcgacg ttgtatggat
     3661 agtcatattc acagtcgtat acttatttgg agtattcccg ggattctaat ggtacattgg
     3721 gagataatat gagtcaggaa ctttctgcag aggaacatac agggcaccca acagctgggc
     3781 aatatttccg catagcagta attttatgtg tcataacagc cgtagaagtc ggagtattct
     3841 atgtcgaatt cttagggcac tggatgattc cgatactgtt tatattgtcg gccagtaaat
     3901 tcaccttggt cgctatgtat tacatgcatt tgaaattcga tcacaagctt tttacctacc
     3961 tgtttgtcgc tggcttcata ttagctgcat taattattag ttttctacta ctgctttttg
     4021 gggtgtattc ttaacctcgc taaacggaaa ccaaacacca aattccattc atgtcgtttt
     4081 taggtttttc tgactcaata tggttgcact ggcaccctca tttagaggtt gtacttggtc
     4141 tgatattggt ccaatgcgcc tacttgtatg gaatcgggcc ataccgaaga agacaaactg
     4201 attctcccga aataaatccc cgccagactg ttatgtttac cctggggacc ttcgttatct
     4261 tcgtctcact tacctcccct ttacacatat taagcgatca ttacttgttt agcgcccata
     4321 tgctgcaaca tgttcttata acacttatat gtcctcctct tctaattgtg ggaacgcctg
     4381 gctggctggc gtcaaagctc ttccaaaata gatggtctat ctcagtcttc aggagaattt
     4441 ttcatccact cggtgctgtt gtgatattca acataatttt ttcaatgtgg catttaccaa
     4501 ttctgtataa tctttcagtc agttaccact gggtacatat cctagaacat tcactcttta
     4561 tatccgcctc tatcgtaatg tggtggccta tttgcagtca ggtccagtct atccctaagc
     4621 tgccctaccc aatacaaatg ttatattttt ttgttatgtc attagctcaa attatagtat
     4681 ttgggataat aacgtttgcc agagaaccaa tttatcaaca ctatattgat gctccacgaa
     4741 tttggaatat aagtccactt gtagatcagc aacttggggg aatcataatg aaggtgggaa
     4801 gtggcttcct cttcctcttc ctgatcataa tcgctttctt caaatggttc gacgaagatt
     4861 ccaattccca ggaaacagcg tataataagc aggattctac agaacgggag ttgcaatgaa
     4921 caaagttacc ttagctctag tggtaccctt agcctctttc gccatgatag cggtatttgc
     4981 cataaccctt ggcttcacct tctatcaaat acatcacaat acgggtttag gaacgatcgg
     5041 agtgatcgtt attgggctag cattattaat cctcactcct ttagtggcct ttcttctcga
     5101 aaaaaagaca tctccataat agctttgttg gtcagggaac tataccccca tagtagtaac
     5161 aaaagcctcc actctctcca tttactattg ttagtggttt tttctctaac ttacctcgtg
     5221 gggtgcacca cctcaggtaa ctctagcttg tacggaacag ctctcaacga tcatgacgga
     5281 tcaagattct ccctcctgga tgagaatgct gttgaagtaa cagaccagac ctacatgggc
     5341 caagtacacc taattacctt tttatttaca aactgcacca gtctttgccc acttgtgacc
     5401 gcaagcatta agcaatcctt ggaaaggtcg gaagacacac gaaatactcc tgtcttaata
     5461 atttcagttg atccaaaggg agacaccatt gaatccagaa aagccttcaa aaatagatgg
     5521 ggcctttcat cgaactggcg ttacctcaac ggaaataaag aggaattaaa cactatctgg
     5581 aagagttttt acctgaatcc acaagaagcc gctatcgaat cccttaacag tcaaatgaat
     5641 agaaagtacg acattatcca ttcatcgcct gtttacatag tagatgatga aggtcgtccc
     5701 gcagtagtac acacaaaccc aatagaccca gatcatttac atgaggatct tatccgaatt
     5761 tcgaaacgtt aagctaaagt cgtcaaacta attatctagg cagcttcaag ggccgttttg
     5821 gcgctttcta ctacacccgt gaatgattca ggattactga tggccatctc agccaacatt
     5881 ttacgattga tatcgatacc tgccaatttt agtccatgaa tcaatttact atatgtgatt
     5941 tcgttcgacc gggcggctgc gtttatccta atattccaca agcgtcgcat atctcccttt
     6001 ttcttctttc tatggtcata tgaatattgc agcgcgtgta tcattgactc atgtgcgcgc
     6061 ctataaaggg tatggcggac accttgatga cctttggtca tattcaatat ctttttatgt
     6121 ctacggcgtc tcgttgtgcc ggttctaact ctagtcacct aaagatctcc tgtctgaaat
     6181 tacgattatt tactgtacgg aagcagtttc ttaagtcgag gtacatcaga ttcgctaaca
     6241 ggcaatgtgc ccgagtacaa tcttttcaca cttttgggtt ttttcctgcg caggtgactt
     6301 cttagacctt tcattctcat caactttcct gatcctgtga ctttgaaacg cctcttggca
     6361 cctttatgag ttttaagttt tggcactttc tggttcccta ttatttttat tcttttttaa
     6421 agatgggctg ggtgccaaaa tcatggctaa aaatttgcct tcaaaagaag gtgctttttc
     6481 caacaatgcg tcttcttgta aatcttctgc cacatttttg agcaaagcca ttcctatttg
     6541 aggatgttgc acctctcgtc ctctaaacat gactgaaaca cgcactttag agccctcagt
     6601 tagaagtctt ttcactaatc tagttttggc ttcccggtcg tggtctccaa ttcgggtctt
     6661 catccgtact tctcgcacag aattattaga tttggttttg gaggcttttc tggcttcacg
     6721 ttctttacga gtggcctcat acctaaattt cccataattc aacaatcgac aaactggggg
     6781 attggccgat ggtgctacct ccacaagatc caaacccttc tcggtcgcca tttctagtgc
     6841 cttgtcagta gtcaactcac caagttgatt tcctttgtta tcaacaacca gcacagtttg
     6901 ggcagttatt tgatcattaa ctcgataatt tttaggtatg ttacttcccc ttttcacaaa
     6961 ataaatagtc cagtgaatac actggaccac aatgaatata gcacacccag tgtacgcgat
     7021 caacaaaatt aggtagcaga tcttttaggt gattttatcc tccacctcag aagaaattct
     7081 tttttttatc tcattcagag aaagggcccc taagtcctct cctgatctca accttacacc
     7141 aacgctttcc gattctacct ctctatctcc cactatgaac atatatggta ctttagctat
     7201 ttgagcatct cgaaccttag cattcattcg ttcacttcga gaatctatgg cggaccttat
     7261 tccactttct gacaattcac tatgcaaaaa agtagcgtag tcaaaatgtc tgtcagcaat
     7321 tgggatgatt tggacttgcg ttggggccag ccacgtagga aaagctccac cgtaatgttc
     7381 gatcaatatt cccataaatc tttccaagga cccgaaaatc gctctatgca ccataaaagg
     7441 ctggtgggga ccaccgtctt ctcctatata agttaatccg aatcgctcag gtaagttaaa
     7501 atcgaattgg accgtagtac actgccagga tctacctatt gcatctctaa tatttacatc
     7561 aattttaggt ccgtagaaag ctcctccagc ctcttcaatt tggtaatcta accccctaga
     7621 atccaaggtt tccataagag atttttctgc caattgccat tgctcatctg accctactgc
     7681 tttatcaggt ttagtggaca aatttaaagt aaagtcatca aaaccaaagg cttccattaa
     7741 atcgaatgta agatccaaaa caccgtctat ttctgtctcg atctgatctg gggtacagaa
     7801 tatatgtgca tcgtcttgag taaatcctct aacacgaagc attccatgaa gtgtacctcc
     7861 tctttcatag cgataaaccg tacctagttc acctattttc atcggtaaat cacgataact
     7921 acgcaaagat gatttgtaat acataatgtg gaaagggcag ttcatgggct ttaaatagta
     7981 attttgccca tctgtttcag caggaggaaa catgttttct tgaaaaaaat ctagatgccc
     8041 gctggtttgc cacaaatttt cccgcccaat attgggcgta tatataaggt catagcccct
     8101 gctatagtgc tccttgcgcc aaaaatcttc aaaaatactt cttaccctgg ctccctttgg
     8161 atgccaaatt atcaaacccg gtccagtttc ctcgtggatg gaaaataaat ctaattctct
     8221 tcctaacgtc ctgtgatccc tacgttgagc ctcctcaagc cttgaaagat gttcatccaa
     8281 agtatcttgg ctttcaaagg ctgtcccata tatccgttgt agcatctgac gattctcgtc
     8341 acccctccag taggctccgg cgacagttag gagcttaaaa gcaggtattt tacccgtaga
     8401 ttccacatgg ggaccagcac atagatcttc gaattctcca tggctgtatg tgctaattac
     8461 ctcttgttcc ggaatatccg atatcacctc taatttgaaa ggttctgact gattcataac
     8521 cgatatctgc tctctagtat attcggcata gataaaaggt aaatcctcag atatgacttt
     8581 tcccatggct acttcgattt ttttaagatc ttcatcagta aaaggggtct caaccatgaa
     8641 gtcataatag aatccatcct cagtgggagg tcctattgca agttttgcct caggataaag
     8701 ttgggtaaca acatctgcca tcacatgggc agtggaatgc ctaatctttt ctctcaaatt
     8761 tttcagttct tcagaattca tgcatacacc taatcaaatt tcatcaaacg aaaacttctt
     8821 tgactaaaat aaccgtaccc gcacatataa ctatacctag gatcaacctt cgatagtagc
     8881 tctgattaat ttttttcaca agcatagaag caataaaaaa accaaggagg gcaggaaaaa
     8941 tacttacacc agtcagtatc agtcgttccg aatcaaacat accagttata aaatagccaa
     9001 tgaccgctgt tgtctcgata aataaataat aatatggcag ggaacctcga attgactccc
     9061 tatcccattc tcgtgacaat gctgcaatgg ccatgattgg ccctccaatc ccagtagagg
     9121 taatcatcgc actcaccaaa aaagcagctc cccacaaggt atatctatgt ctaattgccg
     9181 ctatagttgg atttaagacc ataacaaatc ccagaaaaat aattaaaaga gcaattgaaa
     9241 ctctcagtgc cgaaggattg gagttggaaa gaatgtatac cccaacagga actcccatca
     9301 ttccgaaaat aactgggacc gtcatttccc gataatttat atggcgcctg ttttggtgaa
     9361 tcattaaaac gaagatcaat aatgaaacgg tattgatgat gataacggta gacttcggat
     9421 ccagcgtaag taacatgatg ggagaagacc cgatagctat accaaatcct gctgtcacta
     9481 aaacgaggct gcccacaaat atggccaaaa tcgttagtag aatgtccaca actaaagagt
     9541 ccatttatac tatgggattc tcatgaattc tggatcgggc ttaattagac taacaggaat
     9601 cacatcctca tcatttccct tcatctgcca aaatattttg aattgaggtg aacgtgtcga
     9661 tggtaggctc cgcagttcaa tactggtttt cccgccgtgg atacttattt ctcctgatcg
     9721 agcgaattca ctatcccaag cagtctcagc aagcaaaaca tcattcacaa tcagtttcat
     9781 agcactgttg cttgttaagg aaaatgtgta ttctccagat attggaattt ccacaaatcc
     9841 tgcccatact gcctcatacg gaccttcaat tggcggtgca tagtaaaagg tatcagcagt
     9901 gagtcccaca tgtgaaacaa cgggttgaga ttcctgataa aacactgcag ccaatcccgt
     9961 cggagatcta tcacctaaga aaagtagtgc cgatggtata ggttccatta tattcccatc
    10021 ccgaccccat aacacctcca caaaacctgc ctcagaatgc gcaataccct taaggcgtag
    10081 tgagtgtagt cctacaccca atttaatacc atccccattc tcttgaattg aaataccatc
    10141 gatatatagc tgtccatcac cggacacttg gaattggtaa agtccctgtt cacgcacatg
    10201 taaattactt tggacgacaa aatctacagg aaaagccacc ccagataaat ctttagccca
    10261 cgagtatgaa aaaatatcga atttatctgt ctttacgagg acatcttctc tcttaaacgt
    10321 agcctccact cctaggctat caactaactg ctgcttattt attaggacct cgtataaaat
    10381 tgggtcgact ccatgaggag ccataatttc cctaaatttg gcagacgggt agtattcagc
    10441 caacaggtca taaataccag cgtcgcgggg ttccaaataa attgccgccc catgtagaac
    10501 tcgattggga gaaataggta aatcccttgg agcaaacaac ggctcataat gaacatttcc
    10561 agaaactaca gaagctgtga gactaaaaag aaattgtctt gatgaataca aggtatatcc
    10621 attctgagat tgtttaacca tttcttttgc catcaaggtc tcggaggtag aaaaatctgc
    10681 aaacacgtca gggtgtgatg cttgttttcc aaaatacata ctcacattga aatagcctat
    10741 aaaaccaaac aagacaataa taaaaaccaa acctccttta cgaaaaatat ccctagggtt
    10801 taagttaaaa aggcacagca aatgaactaa tggcacaatg cttataatca gaaccgctgg
    10861 tataactcct attgatcgca acgattgagg tgactcccag ggaaccgaaa atattcctgg
    10921 tgtcaacatt acaaaaaccc agcaaggaaa taataggaac agaggccgtc tccattgggc
    10981 cacaataaga attattccag ctataaacaa tgtacctgta acatcatcca acataggctc
    11041 aaaaggtaag ttatgcctcg gatttgggtc tcccgaaatg tggaacataa atatatgttc
    11101 cacaaaattt ccccatatcg ctccgaacca actcacatct gaaagatgat ttagtatgaa
    11161 tgtttcttca gtccgtttga aaaatatttc tggatttcta aaggaatatt gcagtagggg
    11221 aactgtactg ataattgccg ccaaggccat actgactaaa caatcaacta gccttctcca
    11281 acccggaaac ccggagcata ttctcaatcc tacaccaatc agaataacta tcggaaacaa
    11341 ccgaaacggc gcataaaacc acattcccac ccctagagcc atcccagtcc aaaaaaacca
    11401 aaaagggctc cagttaatta gggatctcca cagcaacaat cctgttagtg cagaaaaaac
    11461 tgctgcagtt ataccgtgca tcccaattcg accccaattc aggctccatc tcataacagc
    11521 gatcccaaaa gctcccccaa gtccccaaaa cgcaccgaat acatcctttg caaaataaaa
    11581 catagccaat atgagaatta cagaaaaccc agccgatatg agtcggccat tcaaccagga
    11641 cacacttgtc aattcctgca acatcgcgat aggaactaag aaagctgatg ggagatgggt
    11701 tgaaggaaca aacactgggg tatttgaagg cgaggcatgt atctggccag ctctgattat
    11761 gttatctgcc tcgtcatacc aaagaccggc gggcaactca cttatgttat aaacacgtaa
    11821 tacaaacccc aaaatcacta cccctgttat taaaaaggtt gtgaaaacag gaatggttaa
    11881 tctggaattt atgaagggtt taaaaaccgg cactgctgac cctctaaaca agaaaggtac
    11941 ggccaataga gccaacatcg aaaacatgta gcaggcccag ccagctccat gggctgccct
    12001 gtcaaataac atgaacccag atatcgataa aatagctgag agcataacta aaaaccagcc
    12061 agcggccttc tcggttaatt tcaaccacag gacttttgaa gatttactgc ctaaactaac
    12121 accggtactt cgagggcaat tatctcttct actaatggga attgcatatt tattagcact
    12181 aattagaata gctatgatcc caacgaagaa ggttgggagt gatatcaaat aaaacccatg
    12241 atcccaaagc ttaacgctga caaagataaa agcgagaccg ctcagtatag acaaaagacg
    12301 aacattgatc tcgtttaatc tctcgatgtg atacatgcct ttatatcacg gtgttactaa
    12361 agatccgttt tggaatatag tatcaatcac agactcatct atttggaatt ccttaagaat
    12421 ttcaaggcta tgttccccta aaaggggggt cggttttacc tgcggtatag gcaaatctgg
    12481 aaattgaatg aggttataag aaaaagcata ttcactacca tcatcaagca cactttttga
    12541 tatcatatcg tttcgaatcg cctgctcatc tttaaagaat cccgcttcat aggcagttat
    12601 tgattccgca catggtatac cgttggaaag catcagttct agccagtatc tagagctatg
    12661 tagctttatg gcctcttcga cctgagaacc taattctgag tgttcccgcc gagaactatt
    12721 ccaatcacga tttgttaatc ccactacatc aagcaagcta tcccctgata ctgaatcctc
    12781 ggcactcaca taaatccatc catcactagt ttgatataac ctgtgaaaat cactaagccc
    12841 attctgggat ttatcggata ttcttctttg tatcttattt ttatatcgtg aaaaatcacc
    12901 ttccaaaagt aagcaccccg cattaagtaa gttagtatta actacttgac caatcccatt
    12961 cttctctttt gcataaaggg ccataactgc ccctagggca cccatacacc cagcggtata
    13021 atcagtggga gcaacaggtg cgagaaaaga tggtggattc ccctcccccc cttgtgcaac
    13081 ttggaggccc atccaagcct gggccaaagg atctaccccg ggcctcttcg caaaagggcc
    13141 atcccaacca aatgcagtca cgtggacttc tatcattttt ggattgaatt ttcttaaggt
    13201 ctcagtatcc aaacccattc tgccggtggc accaggcctt aggttcgcaa ccatgacatc
    13261 gcatgacccg acaatattct gaaggacacc ctgggcaact tcatttttgg catcaataga
    13321 tatcgatctt ttgttggcat ttaaagcatg aaaatatctt ccactagatg gtcgtgagat
    13381 atctccataa ggaggttcta tcttaaggac atcagctcct aaatcagcaa gaagacgccc
    13441 cattgttgga ccggctatca cgttagtgat atcagccact ttgattccat ttaagggtaa
    13501 tttgtcaacg gaatggttct caaggttgtt ttcagtctgt agggatgttt ttagaatagt
    13561 tccactagta gaattcaaca cacgtggccc cttaaccgac cctggtgtat tagaaaactt
    13621 tatgggcagc ccatgttgaa tcattgggcc ataaatcgga tcaattaatt cttgaaccat
    13681 ttggttatgt acgacttgtt gattttcaat cgcggcatct gcggtggcag catgagcaaa
    13741 tggaacatct actgattcaa ataattctgc ccactcatga gatggtcttg ttttcataac
    13801 atttttcact atgtcaaaaa gctcagatct tgcttcttca tccaccgggt tccgcccatc
    13861 tccatatctt ggattaatca taacctcagc tatacccata acagtggcag caatatctac
    13921 gaaccctgcg tgaatgcaac ccagctgaat ccacgcacta tcttggcatt cataaacact
    13981 gtaaaacggc ccacccccca cgggagtaac cttgacaggt cgcggtctaa ctctatcccc
    14041 gatagctttc ggcatataca aaagaccagc agccatcaat gacgtattta tcttcaagcc
    14101 agccccactc ttgagacgat agagcagacc actggccaat cccaacgctg ccaaaagacc
    14161 ggcgcctacg tctatgatgg gatgggcgac atgagttgac ccttcctcaa accctggcat
    14221 gctatctaaa atgcctgttc tagccttgat taaatcagat atcggtgaat gatcctttag
    14281 aagtcctttt tgaccataag gagtgatcga acaatgtaat aatctagagt ttattttccc
    14341 caaagtttga aacccaatct ttttttgcaa tgtatctaag gggagtaaat cctctattat
    14401 gacatcacac ttctgcacaa gtttctcaaa caattcatac tgggtattta gatccaaacc
    14461 gtggagttct tttcccctat ctaaagtggc aaattcagga tgatccctgt tgatcttttg
    14521 ttttgcaggc accctcagaa ctctagctcc attatcactc aaaaacattg agcaaatggc
    14581 tcctgcctta ccagatgtgg tatcgataac attaattccg tcgaatactt ccataattta
    14641 ctaaatttct ccgcaattaa gaaatgtttg acaagcattt taaacaaaaa accccctggg
    14701 ttggatcgaa atctaaccca ggggggttct aaagttcaaa cttattaaag tctaggcaac
    14761 aggggttgct aatttttcct taataatcgc cctcagttct tttactctcg gtaaattcgg
    14821 aaacagacca tcttcctctg ccttatcaaa tatcttctct ccatcagcaa agacctgata
    14881 gatcccaccc gttcccggtg tgattcttat agcaatatct ttccctgctt cagcgaaaaa
    14941 ctcgttggcc atccaggccg ccattccgtg atgcgctcaa gggacgcagt agattatttc
    15001 tacctcaaat tttccttcac ctgccatgtt atcctcctta taagcgtcag ggtggttcac
    15061 taactaactg cgcctaaatt atcacaaatt caaaccatgg gtcaacaagg tttgtaacaa
    15121 cgaaataacc aacaaaagtt aaaattccat atccatgccc atgtcaacta cctctccctt
    15181 ccttgtcttg gaagaatcta atgcactttt aaactccaaa tataactcat actcactttg
    15241 tttatccgag tttttcatgt tcattagatt atctttgaat tcttccacca agttatgttc
    15301 tgtcctccct tcaggcatgt caaagaagtc ccgatcaaat acgggcactt tgccgcttct
    15361 aaaatatcct ttattttcga gttcatacga ttcaagatcg tccgttcctt ttcccaacac
    15421 ttccccagtt tctacataat gttccatcac aggttgcatt ccatattttt gaattggaca
    15481 aactttcata catattccac aacctgaata acgagccatt atcgggcgac aacgcttagc
    15541 aattaattta ttcttctcga ctccccgaaa ccaaaccctt tgtgcttgaa ttgctttgcc
    15601 tggacaccgc ataaagcaaa cttggcaaag ctcacaaaat ttatacatgc cataatcaat
    15661 tggtgaatca tagtcaaggt gtgcatcagt aattatcaac tgtaacctgg cacgagatcc
    15721 aaagtgtggg gaaagcaact ggccgtttac ccccaactga cccaaccctg cctcaacaaa
    15781 catcggaatc atgggaccaa aatgccatgc aggcccagat atttgtgcgc taaaacctaa
    15841 ttcattaatg aaatctgcca gttttaaaga gaggtcagct tgggttaaat aagcatcacc
    15901 ttgtgcaatc tctgaagagt aggaaggtat attttgagtt tcaatatatt cttgttcata
    15961 tgctaaacat ataacattgg gaagattatt tcgaacatag gtcttcctgc tctgataaat
    16021 ataccttcgg tcaaacttcg taaaccctat ctcgctatat ccgagttctt ttgctttact
    16081 tcggattagt tcggtggagt tttccgtctg tgaggaaaca ttgggcgcaa gttcccctga
    16141 tctcttgatt ttgtctatcc agggggtaat tttcttctgg tagtttttgt aaagctcctc
    16201 agtttcaggg gtatgttccg ctctctcttt ttgggcccat acagccgaag cactttgctc
    16261 tattgaaaaa ctctctaaag gaaattctct actgtaataa gaaacctcag tgtctctctg
    16321 cggcactccg tctactttaa ggaggtctgc tgcaatagga atctctatat ccggatcccc
    16381 ctttttaaga cctactcgct ttatgatttt gcctaccttg cgttttgcca aaccctaaaa
    16441 cctctactgt gaatatgcct aatcttgata tgagatggat tataaaacaa gttttccttc
    16501 acaaattctg tgttgtagta aaaatgcaaa taggattatc ctaatatgag tacaaaaaac
    16561 ggggtgaaac ttgactcaag gcctgaagat agaaaaaatc ggtgtatacg gggacaaaat
    16621 tttgatcaga tggacagacg gccacgaaag cacatatggt gccaaagaaa taagaatcag
    16681 ctgtcagtgt gcagaatgtg ttgaagagtg gagtaaaaga caactgttag accctgccac
    16741 cgtaccttca gacctgatag tagaagacca cttaatggtc ggtaattatg caatccagtt
    16801 tttgtggagt gacgcacact atactggtat tttccctttt gacactttgc gaaaaatgtg
    16861 cccctgcaat agttgcgaaa atgtgcccct gcagtagttg cgcttggtta ccaattggtt
    16921 agaaacacac agaactcttt ctgcaatcaa ggaaataaat tctcaaagaa aagggcgagg
    16981 ctattgataa ctcgcccttt tctttgtcac ctcttaggct ggcttgaatt taggaagagc
    17041 tagttcgtct gttatgtctt caaacacaac ttctaaggcc atgccgattt gtaaattctc
    17101 cggcgtggga tcttcaatga caatattagt agccatcttg ggtccttctt ctaattcaac
    17161 aactgccgtc acgaaaggca cgtcatcaac aaaacctgga tggggggctc tttgaactat
    17221 tccgtaagtg aacaaagtag ctttacctga agcttgtatc cagtccgtat ccttcgagaa
    17281 gcagcaagga gaaatatctc gcgggtagaa ataggcattc ccgcaagact tacagttcct
    17341 caaccataat tcaccttgtt ttgccttttg ccaataaaca tccgattccc cttgaggtac
    17401 cggtaatggt ttgttataag ccatgtgtat actccttaga ttaatctact gccagaacag
    17461 tggtgccagt ggaagaaagt gaccctccgg tcccatttat tagacacaat tttggatcgt
    17521 ccaactgtct atcgccacac tctcctctta gctgcctaac cgcttccaaa actaggaaca
    17581 ttccatacat cccagaatgg gtgtaagata gcccgcctcc cgatgtattc atagcaaaat
    17641 ccccacctgg agctgttctt tgattagcta caaagtcagg cgcttcccca ggtccgcaaa
    17701 acccaaggct ttccaaagta gctagtacag tataagtaaa agagtcatat atcattgcga
    17761 gatcaatatc atcgtgagtt acaccagatc tttctaatgc tagtggccca gtgttttttg
    17821 ccactgtacg ggtgagatcc ggcatttgac tgatcagatt gtgatcgtgt gactctgctg
    17881 cacccaaaac ccataccggc tttttctttg catcgcgagc ccgctcagct gatgtgatga
    17941 catatgcccc tccggcatct gtcactaaac aacaatccaa gagatgaaat ggccaagata
    18001 tccatcgtga attatggtaa tcatcaaaag acatatcatc tttcatatag gcttttgggt
    18061 ttagctgggc ccacttgcga gtagccaccg caacctctgc catggcctgg cgggttttat
    18121 cttccccgta ctcgtgcata tatctcctgg ctgccatagc atagttgatg ggcgccccga
    18181 gaaacccata tggtgtttcg aactgtgagc ctggatcagc ggcgtcacca cctgggcgac
    18241 tccttctact ccgacctgcc tcaccgtgag tgacaagtgc cacatcgcaa tatcctgcat
    18301 taatagcagc catggcatga gcgatatgaa ttatgaatga tgagccccca acagcagttg
    18361 tatctgtaaa actcggctca atacccaaat attcagccaa attcaaagta gatgtccccg
    18421 cagtaaaaag cccatttaca tctttcatag acatgcccgc gtcttccaaa gcattgtaag
    18481 ctgcttcagc atggtgagtt agattcgatt tgtcgggaac tttcccgatc tcatcagact
    18541 catccactcc gactatagca accgtttttc ttaaatcggt cattaatttc gtacctccat
    18601 atttagatac aatttagcga atggtactta gcaaccaaat agctgattcg caaagcccac
    18661 tgaggaagaa tacatgtaat tccctatatg cacaaactca ccgaaaccct gatttaccaa
    18721 ttagtgaaag acccatcatt tctcctgagt tgtggaggcc agcctgtagg gttcactata
    18781 cgatccaaag ctaattcttc attgaagtcc actcccatac ccggctcatc aggacaccag
    18841 gcgtaaccgt cttcccattc aatctgcttc ggaaataaat cagttgcata ggacccaggc
    18901 ctcctaggca tttcttgtac acctacattg gttgaggcca tgcatagagc gacacaagcc
    18961 gctgcactca caggtcctaa agggttatga ggcactatgt caatatagtg tgtttctgcc
    19021 cagtgagtta tcttgagagc ttcagttata cctccaacga tgcacaggtc tattcgagca
    19081 taattgatca actcttcctc aatcacttcc ctaaatggcc atttagatgc ccattgctcc
    19141 cccgcggcaa ttggtaaaga tgtatgtctt gccaaagtcc gataactaga tgtgttttct
    19201 gaccttaaag ggtcttctat gaaaaagggt ctgtaaggtt ccacagcttt gcaaaacgca
    19261 accgcgtgtg ctgtatctag tctagtatgc atgtcgaagc aaatctgtat gtcgggacca
    19321 actgcttccc ttatcgtagc gatttgttgt tcagctatac gtatggattc aaccggttcc
    19381 aaggtattta aattatctac atatgcagct gatggacccg tctcaggttg atgccaacgt
    19441 agaaatttcc acccggcatc aatgtgcctt ttacagtttt ccaataactc tgtcattgtg
    19501 tctccttgag tatgaggata gcaaatcact ttatccctta caggacctcc aagcagtttg
    19561 tacaatggca taccaagagc cttggctttg atgtcccaca atccgatatc aattgcacta
    19621 attgcacacg aatatacttt atcggcaggg aaaaatcccc cacgaaacat ttgttgccac
    19681 atcttttcag tgctgaaggg gtcctcccct attaccactt cagatagatg ctcaatagcc
    19741 ttaccaattg ctcctcccca gtttcgtata ccaacttctc ctagaccata aatcccagag
    19801 tctgtctcga cttttacgat gtaatacacc ctgccatctt cgtcagctat agggaagctt
    19861 tttatctgag tcaccttcat ttaattacct cgcagctttc attaactatt tgaatatttt
    19921 ccacatatta gagaaccgta gcgagtacaa tataacaggg caaaatacgg gtcaattgca
    19981 tgtagatatc tgacaaaaaa tgctagtatt tttagatgat ctctttatca gaaagaaaat
    20041 tccaatcagc ccttgatagg gctatggccg atctgtccga tgcgttgaaa aaatgcctta
    20101 aagaaataga catcattatt tacgagatag ccgagaaatc tgatcttcta ggtactaacg
    20161 ttgaaaaaga gctggatttg ctgagccttt atgagatcgt accaatatcc gatggatacc
    20221 acaatgagag gaatttacgc ggtcagctga ctctatttaa aagggcttta gaggcaattt
    20281 gtgaggaaga gtctgacttg gtaccccaat taacaaccat cctagaagaa gaattaagct
    20341 accgaccctc tctctaataa ctcactgttc gaaaatccaa ctccaacagc ctaatatcat
    20401 tccctcaaaa tcctagcatc cccaaccctt ctagtaattt gttgctggtc catgtaatcc
    20461 ataaaaccca aagcatattt tctgcttgtt tgaaaaaggt ctcggaaatc cccaacggtg
    20521 atttgcccat gttgtgatat gtaagcgcgg accccatccg acatttcttt aaatgcttca
    20581 gatgagaaaa ctattccgtc actcacccta actaatcgcc ctttatcaac aaggaaactc
    20641 agaatatctt catctatttc aacatcagtt cttggcgaaa atttgtcttt agctatattt
    20701 tccacataag cttcagacac ccgtttctgt tcttcggtca agtgtgattt atgacctgta
    20761 aaagctatta ttgaggtaaa atttcgcagc aacccgattt cttccaaaac atccacaact
    20821 gcgttaaaac tttctgaaga caaagtcatt ttattcctta attcttctct aggcatacct
    20881 tgccttagag gaaactgtac atgaaacaaa ttaagacttt gtttggtcag atcgcagatc
    20941 tcttcccacc ttttgacagt gaaaaaatac gatttcttaa catctaaaga tgtagacatt
    21001 acggttttct ctgctatcaa aaagtctata gtaggaatta tctcttcttc ttgtagtttc
    21061 gaccctgaaa gtagcgagct caaagtagct ggggaagaag attcaatcat attaagaatt
    21121 atctgcgaag ggatgtctga ttctaaagat tcgagttttt gtatggtagg ggagtgtctt
    21181 ctccgatgtc gtttcgcttg agtatcgaca atactaccac caccaagggt ggtcatattt
    21241 gatcgaataa tgtagtggtc acctctgaga acaggtaatg acttctcagg tttaagttgt
    21301 gcccatgtgc tgttaccagg ttgtacctcc tctccctcca gcaacctcaa acgggctaca
    21361 acttctgaac ttcctgtatg caaggtaaca aacatgttat ggcgtaatgg attaggtgcg
    21421 tcctcaacaa catttagccg aacatcaaaa gcctccgata ggtctataaa atttggggga
    21481 ctcaacacgt ctcctctggt aatttgcgta gattctattc cagatagatt cgctgcaact
    21541 cgtgtaccag gaccaaagct ggtttcggat ttaccgtggg actgaagacc tcttatacga
    21601 gcttttaagc cgatgggaat tatttctatt tcctgaccta ctgacaaaaa tccatcgatt
    21661 aaagtccctg tcaccacagt tccaaatcca ctaatagaga aagatctatc aataggtagt
    21721 ctaggtttat taaagtcctt tttagcagta gttttatcca aggccaaatc tataaaatgc
    21781 ataagatcgg aaatacctga accggtagtt gccgaggcgg atattatttc agctccttct
    21841 agggtggtgc ccgccaataa ttcctctacc tcaccataga ccagctccag ccaatcctta
    21901 tcaaccaaat cttccttagt tacaactacc accccattcg tgacccccaa caagtctaga
    21961 atcgcgaggt gttctctggt ttgcggcatt acagattcat ctgctgctac aacaagcaaa
    22021 gccatgtcga caccgccaac ccccgccagc atattactga caaatttttc atggccggga
    22081 acatcgacta tactgacctc tcttccagac ggaagtgtga tccaagcaaa accaatatca
    22141 atggtcatgc cacggtcctt ctcctcttga aatctgtcag ggtcgatccc tgtaagactt
    22201 tcaacaagtg tagattttcc atgatcaaca tggccggcgg tacctataac gtacacaaca
    22261 aatccttaat ttactgtcaa atacaataca tcaatattac aatagatccg tgcatacata
    22321 tgacgctaaa ttgatacagc cattcggctc tgctaaggta aggtttgata tgaatttaag
    22381 aagtgggagg aaaaatcatg aatgatgcgg tcatagtttc tggggcaaga actccagttg
    22441 gtagattcgg tggagctttc aaagatattg gtgcaccaga tctaggatct actgcaatta
    22501 aggcagctct tgatagagct ggagtatcac ctgatatggt ggatgaagtt gtcttgggaa
    22561 acgcaatcca agctgccgag gccggctatg cagctcgctt agcttcctta caagcaggga
    22621 tccctcatga tacgcccact attgcagtta ataggcaatg ttcatctgga cttgaagcaa
    22681 taaacatggc agctcaatta gtacgaaccg gggaagcgga catcgtggta gcgggcggaa
    22741 cagaaaacat gagccaagta ccctacttaa tagactacaa ggcaaggttt gacggattaa
    22801 gaatgggaaa ttctcaacta atcgatggcc ttaccgaagg cttgggatgt ccagtcaatc
    22861 gatatcacat gggagtcaca gccgaaaatg tcgcagagcg attttccgtg tctcggaaag
    22921 atcaggatga acttgctgtt atgagccatc agcgtgcaag taccgccatt tctgaaggga
    22981 aattcactaa tcaaatagtt actgtatccg tgcctcagag aagaggggac cctatagtag
    23041 taatggaaga tgagggtcct agggcagata caaccataga aggcttatct aatttgaggc
    23101 ctgtttttaa aaaagatggg acagtcacag caggaaatgc ttctagcata aatgatggat
    23161 ctgccgctgt ggtaatgatg tcagctcaaa aagctgctga tttgggtata aaaccaagaa
    23221 tgaagtggca taccagggga atcgcaggag tggacccggc agtaatgggt attgggcctg
    23281 tgcctgccgt ccaaaaggcg atcaataaat caggaatggt cgtaaatgat attgatttaa
    23341 ttgagcttaa tgaggcattt gcatctcagg ctctctactg catccgagag ctaaacctag
    23401 acatagaaaa aaccaatgta aatggcagcg gaataagcct tggacaccca gttggggcca
    23461 ctggcgcaat catgacggta aagttaatgg aagaaatgga aagatccgac tctaccaccg
    23521 gccttgtgac aatgtgtgtc ggaggtggac aaggagtagc caccattttt gaacgcttaa
    23581 attagagg
//
LOCUS       DASCNS010000019         2346 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000180735, whole genome shotgun sequence.
ACCESSION   DASCNS010000019 DASCNS010000000
VERSION     DASCNS010000019.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000180735"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="QF838_00690"
     CDS             <1..348
                     /locus_tag="QF838_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59245.1"
                     /translation="TCEWLTEEELKVYVSEYKRTGFQGGLNWYRSGGSSGNRGNLELF
                     SGMKISKPAMFIAGKQDWGIFQRPGAINKMKNEVCTHMGEIQLVDNAGHWVQQEQPES
                     VLKLLLDFLGGID"
     gene            complement(469..1236)
                     /locus_tag="QF838_00695"
     CDS             complement(469..1236)
                     /locus_tag="QF838_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO59246.1"
                     /translation="MFRNEMKRDLEKGKKVYGTMLQEMVNPTAAQIFKRAGFDFFMVD
                     CEHSPYDQGSVREILRVARLEEICPLVRIRNLDYSLAAGYLDAGAMGLMLPRVENVTD
                     TETLVSFMKYPPEGIRGLSGDAPHSDYRFGDLVEFIEIQNQDTVAIAQIERKAAIENL
                     EEILSVTGIDVALVGPEDLSLSYGVPGQTSHPTVVNAIQKVIDISVKLGVAPGIHMGN
                     IEKLTEWKEKGMQVIMYNSDLGFLIEGAEEGIRALKN"
     gene            1357..2202
                     /locus_tag="QF838_00700"
     CDS             1357..2202
                     /locus_tag="QF838_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024748.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HJO59247.1"
                     /translation="MSRDSLWKQLETILKDKITSFDGVAGLAVKSLTGEKSISINGDE
                     IFPTASTIKIPVLAKLFSLQEEGEIDLSKRIRFTQDMYGAGSGVIHFLENDPEFTVLD
                     VAILMMLASDNTATNTCIDIAGIEEINDLIRDMGLSKTKLRRKMMDPEAINRGDENVS
                     TPNELIMLMDHLHNRKPTPKVSAQVLRIMSKPKSAYLNKAIGSKVVVANKPGGMDKVR
                     GDTGIVYLPNNPYAVSIMSKFSMVDSIAQEGFLIDLARTIHKFMTIVEETNEFGLGLP
                     KTLPL"
BASE COUNT          735 a          497 c          467 g          647 t
ORIGIN      
        1 acctgcgaat ggttaactga agaagagctg aaagtctatg tctcagagta caaacgaaca
       61 ggatttcagg gaggtctaaa ctggtaccga tccgggggaa gtagcgggaa ccggggaaac
      121 ttagaacttt tttcagggat gaaaatatca aaacctgcca tgtttatcgc cggtaagcaa
      181 gattggggaa tattccagag gcccggtgca atcaataaaa tgaaaaatga ggtctgcacc
      241 catatgggtg aaattcaatt agtggataat gctggacatt gggtacaaca agaacaaccc
      301 gaaagtgtac tcaaactcct cttagatttt ttaggtggga ttgattagct tccatccggg
      361 acctacattg ctttcacaac ctcatgacac aggacaaaaa taatcgatca ggctatggaa
      421 gtggaagttt ccactggtct acttatatca aatccatgat cagctatatt aatttttcaa
      481 agcccttatc ccttcttctg caccttcaat caggaatcca aggtccgagt tgtacataat
      541 tacctgcatt cctttttctt tccactcagt aagtttttct atattcccca tatgtatacc
      601 cggtgcgact ccgagtttta ctgaaatatc tataactttt tgaatggcat taaccacagt
      661 ggggtgagag gtctggccgg gtactccata cgatagagat aaatcctcag gtccaacaag
      721 agccacatct atcccagtca ccgaaagtat ttcttccaaa ttttcgattg cggcctttct
      781 ttctatttga gcaatagcca ctgtatcttg attctgaatt tcgataaatt caacaagatc
      841 tccaaacctg tagtcagagt gcggagcatc cccggataga cctctaatcc cttcgggtgg
      901 atacttcata aaactaacta atgtttctgt gtcggtgaca ttctctaccc gtggcagcat
      961 caaccccata gctcctgcat ctagatagcc agccgcaaga ctataatcaa ggtttctaat
     1021 ccggactaaa ggacaaattt cttccagccg agcaaccctt aaaatttccc taacacttcc
     1081 ttgatcgtat ggactatgtt cacaatcaac cataaaaaaa tcaaatccgg ctcgtttaaa
     1141 aatttgagcc gctgtagggt tcaccatctc ttgaagcata gtgccatata cctttttacc
     1201 cttttccaga tctcttttca tttcgttcct aaacattttg tacctctcaa ttcaaactcc
     1261 aagaatagat tcatattgca tatcgtgtcc gattatacgt attagcccat atcctttaaa
     1321 attttatata tcattgaaat ttaaaagagg aattaaatga gcagggattc tctgtggaaa
     1381 caattagaaa ccattttaaa agataaaatt accagttttg acggggtagc agggttagcg
     1441 gtaaaaagtc tcacaggcga aaagagcatt tccattaatg gagatgagat atttcctaca
     1501 gcatcaacaa taaagatacc tgtgttagca aagctgtttt ccttgcaaga agaaggcgaa
     1561 atagacttat ccaaaagaat tcggtttact caggacatgt acggagctgg aagtggggtc
     1621 attcattttc tggagaatga tcccgaattt actgttttgg acgtcgctat actcatgatg
     1681 cttgcatcag ataacactgc taccaacacc tgcatagaca ttgcaggaat tgaagaaatt
     1741 aatgatctaa ttcgagacat gggattatct aaaaccaagc tcagacgcaa aatgatggat
     1801 cctgaggcaa taaacagggg tgatgaaaat gtttctactc cgaacgaatt aatcatgcta
     1861 atggatcact tgcataacag aaagcctact ccaaaagtat ctgcccaagt cttaagaatc
     1921 atgagcaaac caaaatccgc ttatctgaat aaagccattg ggtctaaggt ggtggtggcg
     1981 aataaaccag gaggcatgga caaggttcgc ggcgacacag gcatcgtcta tttaccaaat
     2041 aacccgtatg cagtctccat aatgagcaag ttttccatgg tcgattcaat agcccaggaa
     2101 ggttttctta tcgatcttgc tagaaccatc cacaagttta tgacaattgt ggaagaaacg
     2161 aatgaatttg gactagggtt gccaaaaacc ctaccgttat agaaaaaatg gaaacaagag
     2221 tattaggaaa atctgattta aaagtctcca cggtatgttt tggtgcctgg ccaattggcg
     2281 gtggcatggg aagagtcgac ccccaagaag ctgttaagac tatccacgct gcaataaatg
     2341 ccggca
//
LOCUS       DASCNS010000020         1442 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000188463, whole genome shotgun sequence.
ACCESSION   DASCNS010000020 DASCNS010000000
VERSION     DASCNS010000020.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000188463"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            176..487
                     /locus_tag="QF838_00705"
     CDS             176..487
                     /locus_tag="QF838_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJO59248.1"
                     /translation="MKPVIKNYREHSNPILGKSDGVPNYVMLYVQHAPGESSPFHTHP
                     WEHQAFITEGSGVLFFGGEEYPIKQGDCVHVPEDVEHQFRNTGDVPMNRVTVNPLKSE
                     E"
     gene            complement(490..1380)
                     /locus_tag="QF838_00710"
     CDS             complement(490..1380)
                     /locus_tag="QF838_00710"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HJO59249.1"
                     /translation="MLDSFYGMHWMLATPFDQKEEVDYESIPNLVAEAISSGCTGVVG
                     LGVMGEAARLSDKERTKIAETIIDAAGNLPVTLGTTANGTKAMIERSKEAEQMGAAAV
                     MVSAPSMPKANLDALFGYYYQLAEQISIPIVMQDYPQTSGVEMPVDFVVRVADEIPNV
                     KYLKLEDPPTPTKISAIRNKIQDRLGIFGGLGGVFLLDELRRGSIGAMTGFAYPEVLV
                     QICNHHKAGASNLAEKLFYDHLPLIQFEQQEGIGLAIRKAGIHHRGLISHPIVRHPAG
                     QLAEKTFNELLQIIQRVGLK"
BASE COUNT          394 a          295 c          334 g          419 t
ORIGIN      
        1 caataaaagc tttgatggct cacgtgtctc agatgggtga cagaccatat gatgaggtat
       61 tggaaaggat gatggaatgg cgcggagaaa gaggtaaagg caaaggaatg ccacaggcag
      121 acgcctttaa acggatttat tataggggat aaaaccaaat aacaaaggaa taaaaatgaa
      181 acctgttatt aaaaattaca gagagcactc taacccaatt ttaggtaaga gtgatggagt
      241 cccgaattat gtgatgctct atgttcagca tgcaccaggg gaatcctcac cttttcatac
      301 tcatccatgg gaacaccagg cttttatcac tgaagggtcc ggagtattgt tttttggtgg
      361 cgaggaatat cctataaaac agggtgattg tgtccatgta cctgaggatg ttgagcatca
      421 gtttagaaac acgggggatg tcccgatgaa cagggttacg gtaaacccat taaaatcaga
      481 agagtagatt tattttagtc cgactcgttg aattatttga aggagttcat taaaagtctt
      541 ttcggcaagt tggcctgcag ggtgcctaac aattgggtga ctgattaatc cccgatggtg
      601 gataccggct ttccgaatgg caagaccaat cccttcctgt tgctcgaatt ggataagggg
      661 aagatggtca tagaatagct tttcggcaag attagaagcc cccgctttat gatggttaca
      721 aatttgtacc aacacttcag ggtacgcaaa tccagtcata gctcctatag aacctctcct
      781 tagttcatct agaagaaata ctccgccaag cccgccaaag attcctaacc tatcttgaat
      841 tttattacga atagcactta tttttgttgg ggttggcggg tcttcgagtt tgaggtattt
      901 cacatttggt atttcgtccg ctaccctgac tacaaaatcg acaggcattt ccacacctga
      961 tgtctgcggg tagtcttgca ttacgatggg gatagatatt tgttcggcta attggtagta
     1021 atatccgaat aaggcatcta agttggcctt tggcattgaa ggtgctgaaa ccatgaccgc
     1081 tgctgcaccc atttgttcag cctctttact tctttctatc attgcttttg tcccgttggc
     1141 cgtggttcct aaagttacag gtagattgcc agctgcatct ataattgttt ccgcaatctt
     1201 tgttcgttcc ttatcggaga gacgtgcagc ttcacccatc actcccaaac caacgactcc
     1261 tgtgcagcca gaggatattg cctcggcaac taaattaggt atcgattcat agtcgacctc
     1321 ttctttttgg tcgaaggggg ttgctaacat ccaatgcatt ccgtagaaag agtcaagcat
     1381 atgggtctcc tatgggtagt aaattgttct tatacgatga ttctcgctgt cagctatgaa
     1441 ag
//
LOCUS       DASCNS010000021        18049 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000189586, whole genome shotgun sequence.
ACCESSION   DASCNS010000021 DASCNS010000000
VERSION     DASCNS010000021.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000189586"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /gene="polA"
                     /locus_tag="QF838_00715"
     CDS             <1..2107
                     /gene="polA"
                     /locus_tag="QF838_00715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HJO59250.1"
                     /translation="HLEEVTPPRAKKNLAENKAIADKSQILTRIIRDAPITLDLSAAE
                     FGTFDRDKVVDLFRELEFHSIIPKIPGNKSDFGQNPETQLSFLTEKSETEYILVDNEK
                     SLKELVTKINTDLGFSFDTETTSTDAMNCKLVGISLSIEPSKGWYIPIGHDNGTQLPK
                     GDVLSTLKPIFENPAIPKRAHNANFDIMVLMNDDIRVEGLSFDTMVAAHLSGRRNWGL
                     KELALECFGEEMTSIKNLIGTGKSQITMAEVSIEEAAEYATADADFTERLHDLMKSEL
                     EQKNISNLFYEVEVPLIPILVTMQRNGVKLNENILESMSISLGEQLNQIRSEMFELIG
                     HEFNLNSPKQLSELLFTELNLPPTRKTKSGHSTDAQALEDLKTILDKGDSENADPRSY
                     EVLNRILEHRELAKIKSTYIDALPSMVNKSTGRVHTSYRQTGTTTGRISSNDPNVQNI
                     PVRTELGRKVREAFVTENPDEDVLLAADYSQIELRVLAHLSKDSGLVNAFHRGEDIHS
                     ATSSMVYDIPIDEVTPDMRRVAKVLNFGVIYGLSPHGIARQTDLSQKEGKEFIDIYFN
                     KYPGILNYLEEIKSQCHTSGYIETLLGRRRYLPEINSRNFHLRSQAERAAINMPIQGT
                     AADIIKVAMINIDEQLKVLGMESKMILQVHDELIFEVPQQELQQMESLVGELMPSAVI
                     LDVPLEIEMKTGSDWGNLK"
     gene            complement(2142..3566)
                     /locus_tag="QF838_00720"
     CDS             complement(2142..3566)
                     /locus_tag="QF838_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59251.1"
                     /translation="MNRPLIPFLAISLGVFCMVVDQGGIMLALPSISRFFDSDLSTTQ
                     WVLIGYVLSISASLLPMSRISDLLGHKKIYVWGLTLVAATTIFAGFSNSIGLLIVLSL
                     VRGVGSGMTQGTSMALVLSIFASSQGGKALGLYISVVGIGSGVGPLIAGFILNSYSWR
                     FLYFFLGGLGVISAAAVSLLVRSNHVSSIKFKEFRFDWIGAALFTIGIASLLQTTTWG
                     SRIGFSNPITIGIACLSLAGFAGFIYKEMLVEEPMIDLRLFRNKLFSSGVSAHFLFFA
                     ATSCSFFFMPFFLEIVAGYSPQQIGFIMGAGAISMSIAGAFCGYLSDKFGPFLFSIGG
                     FVVAALGLLVLTNLTVEATWKLAIIGMLSTSMANGIFFGPNNKQILGSVSRSFHGVIS
                     GFMHTTRNSGSAIGISIGTAIATSVMAYMGYEPTLSDLTSDTATSVLDSFVRGIKFVF
                     WGGVVVLILGIFVLVLGGRSKVNN"
     gene            complement(3989..4978)
                     /locus_tag="QF838_00725"
     CDS             complement(3989..4978)
                     /locus_tag="QF838_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59252.1"
                     /translation="MEINLKISFLLPAAFMLLLAACGGGSQIADTPTPETKVPPTATP
                     TATPTPTPSPAPTSVQGVPLTSASAITSPPLKMEGINTHIEMYGTGADPNIITLGPKV
                     GGHPGWEPYLNKTSMEFELNHGVPVLAPIDMVLVGFKNQSAEYRIRSDGQRQTPYNDL
                     LLYFESASSDWPGMIIGSYHLLSSPLLPGHNQNPGCSEIEEWGSRMQAQGHMFLPKED
                     RVMDPTGDALSCDALIGYTVKRGELIGFAGSVGTHSMAPFRFKVSHTSENPTVKRGNR
                     YLHWVQPGSFFYWKCYGAGTEFPSGILAYPFECGGYQLPAEQHDSNFKYSSKK"
     gene            complement(5012..6271)
                     /locus_tag="QF838_00730"
     CDS             complement(5012..6271)
                     /locus_tag="QF838_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HJO59253.1"
                     /translation="MNGIFWVILITLILDFGVGLFSTILNVLSLKSIPPVELEDVYQG
                     EEYRKSQRYTRVQSLFGLTGSTIKFVLLLTFWLSGGFNYIDQTVRLMEWNEIWNGVAF
                     IGILATFGLILNIPLDLYSTFVIEQRFGFNRTTYSTFVLDTLKGVFLYIIIGIPLLTG
                     ILYFFEYSGSLSWLYVWIFLTVISFVISIIGPIWIMPIFNKFTPLESGELRNAIVEYA
                     KKVNFTYGNIYVIDGSKRSAHSNAFFTGFGKTKRIALFDTLIEQLSTDEIVSVIAHEV
                     GHNKKRHVVSGMALSVLHTGVLLFLFSLVMENQSLFEAFSMKSTSIYASIVFFGLLFT
                     PIELIISPVMHFISRRNEYQADQWAVETTTSKNNLISGLKKLAAENLANLSPHPLFVI
                     LNYSHPPLFNRVESVNSLLSCRKESGA"
     gene            complement(6499..7566)
                     /locus_tag="QF838_00735"
     CDS             complement(6499..7566)
                     /locus_tag="QF838_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HJO59254.1"
                     /translation="MKAALFYGGKDIRIEERPTPNPGPGEVLVEIRSAGICGSDLHNY
                     RGNRQSVWENPWEQGHELSGVVSGLGDGVNNLSVGDRVGIEAEHLIGCGSCVQCRRGD
                     YHICSRRGMVNGSRHASHGFSSHDVTFASNCFVLPDGVGFDQAALLDCYACGIHAVNR
                     TAVTPTDTTVIVGAGAIAMTLGQVAKAYGAGRVIMVGTRVETLEVAVRSGAADEFIVN
                     VCNDPVQDVLNLTGATGAEVVFETVGGESQIISQCMQMAGRGGTVSVVGLFTKPQIID
                     SQEYGMAKELRLTWSNSYSSWKGVSEYKSAIDMLGDGLLDANPIITHRFPQRNISEAF
                     AAASDKKSSGAIRVIIDHCLP"
     gene            8219..9700
                     /locus_tag="QF838_00740"
     CDS             8219..9700
                     /locus_tag="QF838_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJO59255.1"
                     /translation="MGNKNQYDVIVIGGGVVGTAITRQLTRYDLNVALIEKNSDVSRG
                     TSGKNSGVVHTGFNVPTGSVKAELNVAGAKHFESLCEELGVPFKRVGKLVVALTKEEI
                     PDLRKLKEIGDANGVPELAIIGEDEVNLREPNVRGIAALHAPTAAITCPYTLTIALAE
                     SAALNGADILLNKEVKKISGVMGGFQIGMGGDSIQSKLIINSAGLYSDQVARLAGVRR
                     YKVWPCRGEYVIIDNAKSNLLTSMVYPVPPKGGSGLGVHITPTIDGNILLGPSAEYVK
                     HKRSDGTSGATVTKLLREAQELLPALDPRDVITSYAGIRPKTVSSSVGGFGDFIIEEA
                     LEVPGMMNLVGIESPGLTAAPAIAEKVSLWVEEKLGLQARSSFVSEKKRSLPFRERSF
                     DCRQDLINEDASYGQIVCRCEMVTRKEIVEAIQNPLGVQTLSGIKYRSRAMMGRCQGG
                     FCSTRIVEIMTGEFKVPSRKITLKGRGSELFIGSTKEARQHED"
     gene            9690..10934
                     /locus_tag="QF838_00745"
     CDS             9690..10934
                     /locus_tag="QF838_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJO59256.1"
                     /translation="MRTEQVDVAVVGGGPAGLAAALSAKESGAEHVLLIERGERMGGI
                     LNQCIHDGFGTKLMGEAFTGPEYAQIFINRLTNTGVNVKLDTMVMDISSDRELVICSS
                     DGLIRVKAGAIVLCTGCRERTRGAIRIPGTRPAGVFTAGVVQEYMNIRNLSVGRKVVI
                     LGSGDIGLIMARRLTLEGVEVECVAEILPYPSGLARNIVQCLEDFEIPLYLSTTVVDI
                     HGIDRVEGITLAKIGPRGGIVRGTKRFVKCDTLLLSVGLIPENELAKKAGIKLSPVTA
                     GPIVDDHFQTSIPGIFCGGNALQVHDLVDYASMESEKAGRSAAFFAGAGKLPSPSIKV
                     LPGQGIRYVIPHEVSGNEPVDFSMRVKSPEADRYIVFKQGEKVIRRMRQIELAPAAMI
                     RMRVRSDRFQRSLDSLTVEVAD"
     gene            10931..11290
                     /locus_tag="QF838_00750"
     CDS             10931..11290
                     /locus_tag="QF838_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019507.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HJO59257.1"
                     /translation="MNEGNIVCTVCPTSCLIDAKWDEKELISIDRAQCKLAWGYISDE
                     IFDPKRVVTTTVPTETGKNPLVSVKTAEPVPKHLVLDIMDTLAVVQAKGLVNAGDVIV
                     ADVLGTGVNIIATSNSD"
     gene            11341..11919
                     /locus_tag="QF838_00755"
     CDS             11341..11919
                     /locus_tag="QF838_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015232707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HJO59258.1"
                     /translation="MLEQNGLAEIRWHGRGGQGVVTAGEIMAEAALDEGRYFQAFPEF
                     GAERTGAPVRAYTRISDEPIDLHCPVSEPSVVVVLDPTLLPSIDVYHGLSNGGVVIIN
                     SPKSPSEIAPQQRLKNHLVATVDATSIAMNTLKRNIPNTAMLGGLLSVTPIVGKDTCL
                     NVIVRRLSMRFPQHIVEANKTAFEQAFSSVQL"
     gene            11965..12282
                     /locus_tag="QF838_00760"
     CDS             11965..12282
                     /locus_tag="QF838_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJO59259.1"
                     /translation="MNPQKKFIAAPGGIIPNPGNTADRQTGDWRVERPVIDMERCTHC
                     MICWIYCPDSAIVAKEGRFIEFDLVHCKGCGICAEECPPNCIDMIEELTFQNINGTSG
                     DEQ"
     gene            12279..13514
                     /gene="porA"
                     /locus_tag="QF838_00765"
     CDS             12279..13514
                     /gene="porA"
                     /locus_tag="QF838_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HJO59260.1"
                     /translation="MKPLPSDSTIEMKDAIGTVKSTAKSLSGGFAVAEAMRQTNPDVV
                     ACYPITPSTLVVEKFSELVANGEVDTEFVAVESEHSALSACLAATAAGVRSQTASSSQ
                     GLAYMWEVLYAVSGLRLPVVLHCANRALSAPINIHGDHTDTMGARDSGWIQLYAENPQ
                     EAYDNALAAVRIAEHPDVLLPVLGSQDGYTVTHSVERVDVLPDKEVKKFVGQYTPKSA
                     LLDIENPVTFGPLVSPKHAFELKLQGIEAMSRAEDVIDSIGEEFAFLTGRPLDSVELY
                     RMEDAELVMVVIGSVAGTARVAIDRLRSDGTKAGLLKIRSYRPFPSTRICEVLGGKKA
                     VAVLDRALSPGAPYSPLYTDICAALYSHGVILPLASFVHGIGGRDTYPKQIIEAFQRI
                     EEKLKVGESMNQTEYLGIT"
     gene            13514..14470
                     /locus_tag="QF838_00770"
     CDS             13514..14470
                     /locus_tag="QF838_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO59261.1"
                     /translation="MLGRQPERARLKLNLREISQEQELLAPGHSLCPGCVESVAVRQI
                     LNVAGPNTVVVTATGCLSVSTSPYPMTAWRNPWIHSLFANAGATVSGIEAAYRSLVKK
                     GVMSDTTPTNFIVFAGDGGTYDIGLQSLSGALERGHRFLYVCLNNEAYMNTGIQRSSA
                     TLPGTWTTTSPFSETVAGKTQARKDMTAIVADHGIPYAAQASPHLWKDLMRKTKKALE
                     SEGPSFLNVLAACPRGWRTEPEIGIRLGKVAVDTCVWPIYEVDDGQWILNSKPREKKP
                     VTEWLELQGRFSHLMRPGREEEVARIQKQIDLLWESLLIRCK"
     gene            14539..15129
                     /locus_tag="QF838_00775"
     CDS             14539..15129
                     /locus_tag="QF838_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59262.1"
                     /translation="MVEDPLETLSQTGMRDQIRSQLLLGELRCWQYVLDLLPNRLKSA
                     GSNLRGPAMFVSKSNSSSTTCGAVMTALMVLGAVRNNGAALQERTIAPMGYLNKQVPS
                     NDIIEIPPSTGITQSDISFGHQLVELISFGAKVDIPIDCQKISGVDWNNPDIETVGAF
                     LAPCGGIAKCTEIIAQAVEGVSQLTSDAAPTDSLQS"
     gene            complement(15523..15687)
                     /locus_tag="QF838_00780"
     CDS             complement(15523..15687)
                     /locus_tag="QF838_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59263.1"
                     /translation="MNSLYPERRYQNEWAVIRGVTLLPPQEGLSIPQLVKVSPAGIDV
                     KHDTYLPIAK"
     gene            complement(15821..16621)
                     /locus_tag="QF838_00785"
     CDS             complement(15821..16621)
                     /locus_tag="QF838_00785"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59264.1"
                     /translation="MSRNRLEGKVAVITGGTSGMGRAAAELFASEGAKVAIAGRRIEK
                     GEMVVNRIRDNEGSAIFIQADVSITKDVITMVDRTMSHYETIDVLFNNAGINNSGDGD
                     PHNEDESVFDEVMGINIKGAFLCTKYVVPHMMNGGGGSIINNSSVLDTRANSSSSTSY
                     HVSKGGMAMLTKKSALSYAKYNIRVNSVQPGAIATEMGGIDWDDLEDTELINKKKKLQ
                     PLERMGHPMDVAYAALYFASDESNFVTGSSLFVDGGKSAVLGEWNENG"
     gene            17061..17753
                     /locus_tag="QF838_00790"
     CDS             17061..17753
                     /locus_tag="QF838_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HJO59265.1"
                     /translation="MNWLARKKRGILQNPIPTGLMGDLKREYKNLAEDKPSLNLKLSD
                     IHRILDHSKGRKQEVIVHLQSLHGLRISEALAIRYEDIDFINNKIIVRNQTSKGRLVP
                     VKTKSARRSIPLQEVTREILENSPNAMTGGFVCKDSNGGPLRYSNYLNRFFTPMIKEL
                     GLRFTTHNLRTFFASWWLVEGKVDVVTVSKWMGHSSPTVTMDIYAKTIEESENRYDNR
                     IGNAVMPPTRAA"
     gene            17881..17956
                     /locus_tag="QF838_00795"
     tRNA            17881..17956
                     /locus_tag="QF838_00795"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17914..17916,aa:Gly,seq:tcc)
BASE COUNT         5376 a         3854 c         4083 g         4736 t
ORIGIN      
        1 ccacttagaa gaagtgaccc ctccaagagc aaaaaaaaat ttagcagaaa ataaagcaat
       61 agctgataaa agccaaattt taaccagaat tattagagat gcgccaataa ccttagactt
      121 atcagcagct gaattcggaa catttgatag agataaggtt gtagatcttt ttagagaact
      181 agaatttcac tcgataatac ccaaaattcc aggtaataaa agcgattttg gccaaaatcc
      241 tgaaacacaa ctgtcgttcc taactgaaaa atccgaaaca gaatatatcc tggttgacaa
      301 tgaaaagtca ttaaaggaat tagttacgaa aataaatact gatttaggat tttcatttga
      361 tacagaaact acttccacag acgccatgaa ttgcaaatta gtgggcatat cactctcaat
      421 tgagcccagc aaaggctggt atatcccaat tggtcatgac aatgggaccc agttacccaa
      481 aggtgatgtt ctatccactt tgaaacctat ttttgaaaat ccggctattc ctaaacgggc
      541 ccataacgcc aattttgaca tcatggtcct aatgaatgat gacattagag tagaaggttt
      601 gtcatttgac actatggttg ctgcccattt atccggtaga aggaactggg gattaaaaga
      661 actggcttta gaatgttttg gagaagaaat gacctctatc aaaaatctga taggaactgg
      721 aaaatcccaa ataacaatgg ctgaagtctc gatcgaggag gcagcagaat acgcaactgc
      781 agacgcggat tttacagagc gtctccatga tttaatgaaa tccgaattag aacaaaaaaa
      841 tatttctaac ctattttatg aagttgaagt tccattaatt cctattctgg tgaccatgca
      901 gcgaaatgga gttaagttga acgaaaatat tttagaaagt atgtcaatct ctttaggtga
      961 acaattaaac caaattagat ctgaaatgtt cgaactaatt ggtcatgaat ttaatttaaa
     1021 ctctccaaaa caactgagtg aattactttt taccgagctt aatctgccac caacaagaaa
     1081 aacaaaaagt ggacactcaa ctgacgccca agcattggaa gatcttaaaa ccatattaga
     1141 taaaggcgac tctgaaaatg ctgacccaag atcatatgaa gtactaaacc gaattttaga
     1201 acatcgtgaa ttagccaaaa ttaagtcaac ctatatagat gcacttccta gcatggttaa
     1261 caaatcaaca ggtcgtgttc acacttccta tagacaaacc ggtacaacca ctgggcgcat
     1321 atctagtaac gatcctaatg tccaaaatat tccggtacga acagaacttg gcagaaaagt
     1381 tcgagaagcc tttgtgactg aaaatccaga tgaagatgtg ttactggcgg ccgactattc
     1441 acagatagag cttagagtgc tagcgcattt atccaaagat tcaggattag taaatgcatt
     1501 tcaccgtggg gaagatatac atagtgcaac tagctcaatg gtttatgaca taccgattga
     1561 cgaagtcaca cctgacatga gacgagtggc caaggttcta aactttggag ttatatatgg
     1621 attaagccca catggaatag caaggcaaac cgatttaagc caaaaagaag gaaaggagtt
     1681 tatagatatt tatttcaata aatatcctgg tatattgaac tacttagagg aaatcaagtc
     1741 acaatgccat acaagtggtt atattgaaac actactcgga agaagaagat atttaccgga
     1801 aataaattct agaaatttcc atctaagaag ccaagctgaa agagctgcca ttaatatgcc
     1861 aatacaagga actgccgccg atattataaa agtagcaatg atcaacattg atgagcagct
     1921 gaaggtgttg gggatggaat caaaaatgat acttcaagtt catgatgaat taatatttga
     1981 agtacctcaa caggaattac agcaaatgga gtccttggtc ggagaattaa tgccttcagc
     2041 agtaattctt gacgtgcctt tagaaatcga aatgaaaacg ggttccgatt ggggtaattt
     2101 aaagtaaacc aatatctaaa taattgactc caatccggaa cctagttatt gactttactc
     2161 ctaccgccga gaactaatac aaatatccca agtatcaaaa ccacaacacc tccccaaaag
     2221 acaaacttga tgcctctgac aaatgaatct aatacagatg tcgcagtgtc actagtcaaa
     2281 tcagacagag taggctcata ccccatataa gccatgactg atgtggctat agccgtgcca
     2341 atggagatac ctatcgcgct gccactattt ctggttgtat gcataaaccc agaaatcacc
     2401 ccatgaaaac tacgggaaac tgatccaagg atctgtttat tattgggccc aaaaaatatc
     2461 ccattggcca tacttgtaga caacattcct ataatcgcta atttccaggt agcctccact
     2521 gtcaaatttg ttaaaactaa aagaccgaga gccgccacaa caaaaccgcc tattgaaaaa
     2581 agaaaagggc caaatttatc ggataaatac ccgcaaaaag cacctgcaat cgacatggat
     2641 attgcccctg cacccataat aaacccaatt tgttgaggag aatatccagc tacgatttcc
     2701 agaaagaatg gcataaagaa aaacgaacaa gaagtcgctg caaaaaaaag gaaatgtgcc
     2761 gatacaccgg aagaaaatag cttgttccga aatagcctca aatcaatcat tggttcttcg
     2821 actaacattt ccttgtaaat gaaaccggcg aatccagcca aggataaaca tgcaatacct
     2881 attgttatcg ggtttgaaaa tccaatccta gaaccccaag tagttgtttg gagtaagctc
     2941 gctattccaa ttgtaaaaag tgccgcgcca atccaatcaa atctaaattc cttgaatttg
     3001 atcgatgaga catgatttga tctgactaac aaagatactg ccgcggccga gatgacaccc
     3061 agtcccccca agaagaaata taaaaacctc caactgtagc tattaagaat gaatcctgct
     3121 attaaaggtc cgactccact accgataccc acaacagaaa tatagagtcc taatgccttt
     3181 cctccttgcg aagaagcaaa aatagacaaa accagggcca tagaggtgcc ttgtgtcata
     3241 ccgcttccta cacctctcac cagactaaga actatcagaa gccctattga attactgaat
     3301 ccagcaaaaa tggtagtcgc agccactaag gtcaatcccc aaacataaat tttcttatgt
     3361 cccaacaaat cagaaatcct gctcatcggc aggagtgagg cgctaatcga caaaacatag
     3421 ccaattaaca cccattgagt cgtagaaaga tctgaatcaa aaaaacgtga gatggaagga
     3481 agagccaaca taattccacc ctggtctacc accatacaaa acacacccaa agaaattgcc
     3541 agaaaaggta ttagcggtct attcattcga actgtatttt aaccaagggg gttgtcacaa
     3601 gagtacattc tatctcaaag ccatcattac agatatttac gattattttg gtttatcgga
     3661 ttattgtcat gacataggta aaaataaggg aagaggaagg agaggaatac tatcaaaaat
     3721 ggaagcacta gctttggtca aggtttgcat gggaggacta tatgagggtc gctgggtaga
     3781 gggctcagca ttaacagggc cgtatgttaa accgctatgc catgggtccg cattcatcta
     3841 tgcccataca agtacatgag ggatgtggca aaaaatgtgg caaaagttaa accccaacta
     3901 tcaatgcttc gtttatatgc tgagcaggtg aatagtgagg atgctattag ggcttacaag
     3961 gctgttgtga ggtaggatgg cacatcagct atttctttga agagtacttg aaattggaat
     4021 catgttgttc tgctggaagt tgataccctc cacactcgaa aggataagca agtatcccac
     4081 tgggaaattc cgtgcctgca ccataacatt tccaatagaa gaatgagcct ggctgaaccc
     4141 aatgaagata gcggtttccc cttttaacgg tcgggttttc tgatgtatgt gatactttga
     4201 accggaaagg ggccatcgag tgagtaccaa cgctaccagc gaatccaatt agttccccac
     4261 gctttactgt gtatccaatc aaagcatcac atgaaagagc gtctcctgta ggatccatga
     4321 ccctgtcctc cttaggtaag aacatgtggc cttgagcctg catcctgctt ccccactctt
     4381 caatttcact acaacctgga ttctggttgt gaccaggcaa cagtggcgaa ctcaataaat
     4441 gatagctacc tataatcatc cctggccaat cagagcttgc agattcgaaa tataggagta
     4501 aatcattgta tggggtctgt cgttgtccgt ctgagcgaat ccgatattcg gcactttgat
     4561 tcttgaaacc taccaacacc atatcaatag gtgcgagtac gggtactccg tgattgagtt
     4621 cgaattccat actggtttta ttgaggtatg gttcccaacc tggatggcca ccaactttgg
     4681 ggccaagcgt gatgatattg gggtcagccc ctgtgccgta catttcaata tgggtattga
     4741 taccttccat ttttaggggc ggtgaagtga ttgcggatgc gctcgtaagt ggaacacctt
     4801 ggacagacgt tggcgctgga ctaggtgtcg gagttggagt agcggtaggt gtagccgtag
     4861 gtggcacctt agtctcaggg gttggggtat cagcaatctg acttccccca ccacaagctg
     4921 ccagcagcaa cataaatgct gcgggtagaa ggaatgatat tttcagatta atctccatac
     4981 ctccccgttg tctctaatcc cccatgggac tctatgcccc agattccttc cggcaactaa
     5041 gcagcgaatt tacggattct accctgttga ataacggtgg atgagagtag ttgagtataa
     5101 caaacagcgg atgtggtgaa agattggcaa ggttttcagc agccaatttt tttagcccag
     5161 atatcaagtt atttttgcta gtggtcgttt ctaccgccca ttgatctgct tggtattcat
     5221 ttcttcttga aataaagtgc atcaccggag aaataattaa ctcaatgggg gtaaacagca
     5281 ggccaaaaaa cacaatactg gcgtatatag acgtgctctt catcgaaaac gcctcaaaca
     5341 atgattgatt ttccatcact agggaaaata ggaatagaag aactcctgtg tgcaaaacac
     5401 ttagagccat tccagacaca acatgtcttt ttttattatg cccaacttca tgagcgatta
     5461 cggatactat ttcatctgtg cttaattgct cgatcaacgt gtcaaaaagg gctatacgct
     5521 tagtttttcc aaaccccgta aagaatgcat ttgaatgagc acttctttta gaaccgtcaa
     5581 tcacatagat gttcccatat gtgaaattca ctttttttgc gtactcgaca atggcatttc
     5641 tcaactcacc ggattcgagt ggagtgaatt tattgaatat cggcataatc catatggggc
     5701 caatgattga aatgacaaac gatataactg ttagaaatat ccatacatac aaccaagata
     5761 aagaacccga atattcgaag aaataaagaa tgcctgttaa gagaggaatg ccaatgatta
     5821 tataaaggaa cacacccttt aacgtatcta atacgaacgt cgagtatgtt gttctgttaa
     5881 acccaaacct ttgttcaatt acaaaagtgg aatatagatc tagtggtata ttcagtatca
     5941 aaccaaacgt tgccaatatt ccgataaatg cgacgccgtt ccagatttca ttccattcca
     6001 tgagacgcac tgtttgatca atataattga accctcctga aagccaaaat gtcagcaaaa
     6061 gtacgaactt aatagtgcta cctgttaatc caaaaagaga ctgtacacgt gtataccttt
     6121 gtgacttgcg atactcttcg ccttgataca cgtcttctaa ttcaaccggt gggattgatt
     6181 tcaaagaaag cacgttgagg atagttgaga ataatccaac gccgaaatcc aatatcaggg
     6241 ttattaagat tacccaaaat attccgttca tattcctccc ttggtgcgaa cagtagtgaa
     6301 agttctcatt agggtggatt atattccctt aacgtagtgg cattttgact gccacacggt
     6361 gggtaaaatc ttcatatggg tatgtgggtg cggtgttata ggggttgtta cagtgtgtga
     6421 ctgagggaga taaagtctat gttgtgatgg gcaaactacc ttttaaaaaa gtacccggtg
     6481 aacttcaaat tcggataact atggcaagca gtgatcgatt atcactctta ttgcaccgga
     6541 agatttcttg tcactagccg ctgcaaatgc ctccgagatg tttcgttgtg ggaatcgatg
     6601 tgtaatgatc ggatttgcat ccaagaggcc atcgccaagc atatcaatgg cagatttgta
     6661 ttcagaaacg cccttccacg acgaataact attggaccag gttagtctga gttctttcgc
     6721 catcccatac tcctgagaat ctataatctg tggcttggtg aaaagcccaa ccacactgac
     6781 tgtaccccct ctgccagcca tttgcataca ctgagaaatg atctggcttt ctccccccac
     6841 tgtttcaaag acgacctcgg ctcccgttgc tcccgtgaga tttagaacat cttggacagg
     6901 atcattacaa acgttcacta tgaattcgtc tgctgcgcca gatctgacgg ctacctccag
     6961 agtctcgacg cgagtgccaa ccatgatcac cctgcccgca ccatatgctt tcgctacctg
     7021 gcccaaggtc atagctatgg cacctgctcc aacaatcacc gtggtgtccg taggtgtgac
     7081 cgcagttcga tttacagcat gaatgccgca tgcataacaa tcgagcaaag ccgcctggtc
     7141 aaaacccact ccatcgggta atacaaaaca attggaagca aaagtaacat catggctcga
     7201 gaatccgtga ctcgcatggc gagacccatt gaccatccct cgtcgagaac atatgtggta
     7261 gtcaccccta cgacactgca cacaactccc gcaaccaatt aaatgttcag cttcaatacc
     7321 tacacgatcg ccaactgaaa ggttgttcac cccgtcccca agaccggaga ccactcctga
     7381 caactcgtgt ccctgctccc aggggttctc ccaaaccgac tggcgattcc ccctgtaatt
     7441 atgaagatcc gagccgcaaa tgcctgcgga tcgtatttca acgagaactt ctcctggacc
     7501 tgggttcggc gtgggcctct cttcgatcct tatatccttt ccgccgtaaa agagggctgc
     7561 tttcattatt tactccacac gtcgctagtt gagctgggat tatagaggca cctagatggt
     7621 atcacctgat actgccaata tcgtagaaca cgaatcggct ataatctagc catgcaaaag
     7681 aacgctggga acagcacgaa acgctactta ataagaggag tcagccaccc ggacagtgct
     7741 gccgaatcgt gcctccagtt taggaattgt actaggggag atttagaagg tcactttaag
     7801 ggttaaattg tcttcatttg tactcaacac aatggctaat cttcactaac agtcttagcc
     7861 cttatgcata catcctcaag ggtttgtgac catttcgtga ccataattta tagtggcacc
     7921 aagagtttaa gatggcgacc ccggccagac tctaactggc gagctcagcc ttaacagggc
     7981 cgtctgttaa cagccgacac cacataacat ttctaatgcg tgagttatta actagtccaa
     8041 gacctcatat agctctcatg tggttagctg ttttaagcca ttgggagtgg ttatagtgtg
     8101 taaaagtaac cgggaaaatc tggaagtata tggtgcttgt aggacgccga ttgaatttca
     8161 ggctcgtata tattcctgaa atctgtggga gtatgaatcg gatgaaagct aataaataat
     8221 gggaaacaaa aatcagtatg atgtcattgt aattggtggt ggtgtggttg ggactgctat
     8281 aacacgtcaa cttacacgat atgatctcaa cgtggcccta atagagaaaa atagcgatgt
     8341 gtcgagaggt actagcggaa aaaacagcgg tgttgtgcac actggtttca atgtaccgac
     8401 gggctctgta aaagccgaat taaacgttgc cggagcgaag catttcgaat cactttgtga
     8461 agaacttggt gttcctttta aaagggtagg taagttggtt gttgctctaa caaaggaaga
     8521 gatacccgat ttacgaaaat tgaaggaaat tggagatgcc aacggcgtcc ctgaactggc
     8581 aattattggg gaggatgagg tcaaccttag ggaaccaaat gtcagaggga tcgctgctct
     8641 gcacgcaccc actgccgcca ttacatgccc gtacaccttg acaatagcct tggcggagtc
     8701 cgcggcattg aatggggctg atattttgct gaataaagag gtgaagaaaa tttcaggggt
     8761 aatgggcggt ttccaaatcg gtatgggcgg ggattctatt caaagcaagc tgattattaa
     8821 ttcagcaggt ctttactctg accaagtcgc aagacttgcg ggtgttcgta gatacaaagt
     8881 gtggccttgc agaggcgaat atgtaattat cgacaatgcg aaaagcaatt tattaactag
     8941 catggtttat cctgtacctc cgaaaggagg atcggggttg ggggtgcaca taacgcccac
     9001 gatcgatggc aacattcttc ttgggccgag cgccgagtac gtaaagcaca agagaagtga
     9061 tgggacaagt ggagcgaccg tgactaaact actgcgagag gcccaggaat tgctgccagc
     9121 ccttgacccc cgcgatgtga taacttctta tgcagggatt cggccaaaaa ccgtaagctc
     9181 ctcagttggt ggttttggcg attttattat agaggaagcc ttggaggttc ccgggatgat
     9241 gaatcttgtc ggtattgaat cacccggatt gacagcagca ccagcaattg cagaaaaggt
     9301 tagcctatgg gttgaggaaa aactaggcct tcaggctagg tcgagttttg tttcagaaaa
     9361 aaagagatcc ctcccgtttc gagagcgatc ttttgattgc cggcaggatt tgatcaatga
     9421 agacgccagc tacggtcaga tagtatgccg gtgcgagatg gttacaagga aggaaatagt
     9481 tgaggcgatt cagaacccgt taggggttca aaccctaagc gggataaagt accggtctcg
     9541 ggccatgatg gggagatgcc aaggaggatt ttgttccacc cgtatagtgg aaataatgac
     9601 gggtgaattc aaggttcctt caaggaaaat cactctcaag ggacgagggt cagagctatt
     9661 tataggatcg accaaagagg caaggcaaca tgaggactga gcaagtagat gtcgctgttg
     9721 taggtggggg gccggcgggt ttagcagctg cattgtccgc caaggaatct ggcgcagaac
     9781 acgttctcct tatagaaaga ggggagagaa tgggtggcat tcttaatcaa tgtattcatg
     9841 atggttttgg cactaaacta atgggagaag ccttcacagg ccctgaatac gctcaaattt
     9901 ttatcaacag gctcaccaac accggagtaa atgtcaaact tgacacgatg gtcatggaca
     9961 tttcatcaga tcgtgagtta gtcatttgtt ccagtgatgg gctaattcga gtcaaggcag
    10021 gcgccattgt tctttgcact ggctgtagag aacgcacacg gggagcaatc aggattcctg
    10081 gaacacgacc cgccggtgtg tttactgccg gtgtggtgca agaatacatg aatatcagaa
    10141 acttatccgt cggtcgtaaa gtagttatac tcggatcggg cgatatcggt cttatcatgg
    10201 ctcgtcgctt gactctcgaa ggtgtggagg tcgagtgtgt tgcagagatc cttccctacc
    10261 ccagtggatt agccagaaat atagtccaat gtttagaaga tttcgaaatt ccactctatt
    10321 taagtaccac ggttgtggat atacatggaa tagatcgggt cgaagggatt accctcgcca
    10381 aaattggtcc tcgtggaggg atagtccgcg gaaccaaaag gtttgtgaag tgcgatacat
    10441 tactgttatc ggtcgggctt attccagaaa atgaattagc aaaaaaagca ggaataaaac
    10501 tcagccccgt tacggcgggc cctatcgtag atgatcattt ccaaacatcg atacctggta
    10561 ttttttgcgg tgggaatgct ctacaagtgc atgatcttgt agattatgca tcaatggaat
    10621 cagagaaagc cgggcgtagt gcagcttttt tcgcgggtgc tggcaaacta ccgtcaccga
    10681 gtattaaagt acttcccggt cagggtatac ggtatgtcat accccatgaa gtgtccggaa
    10741 atgagccggt agatttttcg atgagagtta aaagcccgga ggcggatcgc tatattgttt
    10801 ttaagcaggg agaaaaagtg attcgaagaa tgcgacaaat tgaattggca ccagcagcga
    10861 tgattagaat gcgcgtaaga tcagatcgtt tccaaagaag tctagattcg ctaacagtgg
    10921 aggtagcaga ttgaacgaag ggaatattgt ttgtacggtg tgtcccacaa gttgtctaat
    10981 agacgcgaaa tgggatgaaa aagaattgat cagtatcgac agggcccaat gtaaactggc
    11041 ctggggatat atctctgatg agatattcga tccaaaaagg gtagttacca cgacggtgcc
    11101 tacggaaacg ggcaaaaatc ctttagtgag tgtcaaaaca gctgaaccgg tgcccaaaca
    11161 tctggttcta gatatcatgg atactctagc tgttgttcaa gcaaaagggc tggtgaacgc
    11221 tggtgatgtt attgtagcgg atgttctagg gacaggagtg aatattatag ctacaagtaa
    11281 ttcagattga gagtttaaat gatgcgacag cacacagtta acgatattaa aaagtgttcg
    11341 atgttagaac aaaatggcct cgccgaaatt agatggcatg gcaggggtgg acaaggtgtc
    11401 gtgacggccg gagagataat ggcggaagcg gcattagatg aggggagata cttccaagca
    11461 tttcccgaat ttggagctga gcgaacagga gctcctgtaa gggcatacac tcgtatcagc
    11521 gacgagccca ttgatcttca ttgccccgtt tcagagcctt cagtagtagt cgtgttagat
    11581 cccactttac tgccatccat tgacgtttac catggcctaa gcaatggagg agttgtaata
    11641 attaattcgc cgaaaagccc ttctgaaatt gcacctcaac agagattaaa aaatcacctc
    11701 gttgctacag tagatgctac ctcaattgca atgaacactt taaagcgaaa catcccaaat
    11761 acggctatgc tgggaggatt attgagcgtc acccctatcg tgggcaaaga cacatgttta
    11821 aatgttatcg tacgtcggct tagcatgcga ttccctcagc acattgttga agcaaacaag
    11881 accgcttttg agcaagcctt ttcatctgtt caattgtagt caggttttga ggtatgtgat
    11941 tctaaaaact aatggagata gttaatgaat ccccaaaaaa aatttatagc tgctccgggg
    12001 gggattatcc caaaccctgg taatacggcc gatcgacaaa ccggtgactg gcgggttgag
    12061 cgtcctgtta tagatatgga aaggtgcact cactgtatga tttgttggat ttactgtccc
    12121 gacagtgcta ttgtggcaaa agaaggtaga ttcatcgaat ttgacctcgt ccactgcaaa
    12181 ggatgtggaa tatgtgccga agaatgcccg ccaaactgca ttgatatgat cgaagaattg
    12241 acctttcaga atatcaatgg aacctccggt gacgaacaat gaaacctctg ccctcagatt
    12301 caactattga aatgaaagat gccatcggaa ctgtaaaaag taccgccaaa agcctcagcg
    12361 gaggtttcgc agtggcagag gcaatgcgcc aaaccaatcc ggatgtggtt gcttgctacc
    12421 caattacccc ctcgacgctt gttgtagaaa agttcagtga actcgtagca aatggggaag
    12481 tcgacacaga gttcgttgca gttgagagtg aacattcagc gttgagcgca tgccttgcag
    12541 ccaccgctgc tggtgtacgc tctcagactg cttcttcgag ccaaggactc gcctacatgt
    12601 gggaggtgct ttatgcagtg tccggcctga gattaccggt ggttcttcat tgcgcaaacc
    12661 gcgctctgtc cgctccaatc aacatacatg gcgatcatac tgacactatg ggcgctagag
    12721 atagtggatg gatccagctt tatgctgaaa acccccaaga agcatacgac aatgcgctgg
    12781 cggctgtgag aatcgcagaa cacccggatg ttcttctccc agtattgggc tcgcaagatg
    12841 gctatacggt cactcacagc gtcgaaaggg tggatgtttt acctgataaa gaagtgaaga
    12901 aattcgtcgg tcaatatacg cccaaatcag cgttgctaga tatcgagaac ccggtaactt
    12961 ttggcccttt ggtgtcaccg aaacatgcct ttgagttgaa gcttcaagga atagaggcca
    13021 tgagcagggc tgaagatgtt atcgatagca tcggagagga gtttgccttt cttactgggc
    13081 gtcccttaga ttcagtcgaa ctgtatagaa tggaagatgc ggaattagtt atggttgtga
    13141 ttggatcagt tgcaggaact gcccgcgttg caatagatcg tcttcgatcc gatggcacta
    13201 aagcaggcct attgaaaatt cgctcttatc ggccttttcc atcaaccaga atttgtgaag
    13261 ttttaggtgg gaaaaaagct gtagcagtat tggatcgagc gctatcccca ggtgcaccct
    13321 atagcccact ctatacagat atctgtgcag ctttgtattc tcacggcgtg atattaccgc
    13381 ttgcaagttt tgtgcatggg attgggggcc gtgatactta tcctaaacaa attattgagg
    13441 cttttcagcg cattgaggaa aaattaaaag tgggggagtc catgaatcaa acagaatatt
    13501 taggaataac ctgatgcttg gccgtcagcc ggagagagcc aggttgaaat taaatcttag
    13561 ggaaatttct caggagcagg agcttttggc tcccgggcat agtttgtgtc cggggtgcgt
    13621 tgaatcggtt gctgttaggc agatattgaa cgtagctgga ccaaataccg ttgttgttac
    13681 tgccactgga tgcctttctg tgtctacctc gccttatccc atgacggcat ggcgaaaccc
    13741 ttggatacac agtctatttg ccaatgctgg ggccacggtt tcagggattg aagcagcata
    13801 tcgcagctta gtaaaaaaag gagtaatgag cgacactact ccaactaatt ttatagtttt
    13861 cgccggagat ggtggaacgt atgatatagg actgcaatcc ttgagtggtg cacttgaacg
    13921 aggacaccga tttctatacg tttgtctcaa taatgaggcc tatatgaaca ccggcataca
    13981 gcgctcgagt gcgacattgc caggtacatg gaccaccaca tccccctttt ctgaaactgt
    14041 cgctggaaaa actcaagcgc gaaaagatat gactgccatc gtggcagatc atggaatacc
    14101 atacgcagct caggcgtcac cacatctttg gaaagatctg atgcgaaaaa cgaagaaagc
    14161 tcttgaatct gaaggcccat cctttctcaa cgtcttggct gcttgtccaa ggggctggag
    14221 aaccgagccc gagatcggaa ttcgattagg taaagtggct gttgacacgt gtgtatggcc
    14281 tatttatgaa gtggatgacg gccaatggat tttaaattcg aaacctcgcg aaaaaaaacc
    14341 tgtaactgaa tggcttgaac tacaaggaag attttctcat cttatgcgcc ctggtcgtga
    14401 agaagaggta gcgagaattc agaaacaaat cgatctactt tgggaatctt tgctgatcag
    14461 atgtaaatag tgcaagatct ggaagagaaa accctgtcgc gtaatggata gaggctgact
    14521 gaattggagg aaatattaat ggttgaagac ccattggaaa cgctatctca aactggaatg
    14581 agagatcaaa taaggtctca attgttactt ggtgaacttc gatgctggca gtacgttctt
    14641 gacctgcttc caaaccgact taaatctgct ggatcgaact tgagaggccc agcaatgttt
    14701 gtcagtaaat caaactcttc gtccactact tgtggggctg tcatgacagc tctgatggtt
    14761 ctaggggctg tgagaaataa cggagcagcg ctacaggaac ggaccattgc gccaatgggc
    14821 tatttgaata aacaagtccc atccaacgat attatcgaaa ttcctccttc aacaggtata
    14881 actcaatctg atattagttt tggacatcag ctggttgagt taatttcatt tggtgcaaaa
    14941 gtggatatcc cgatagactg ccaaaagatt tctggcgtgg attggaacaa cccggacatc
    15001 gaaacagtgg gggctttttt ggcaccatgt ggtggcatag ccaaatgcac agaaattatc
    15061 gctcaggctg tggaaggggt ctcacagcta acgtctgatg ctgcccccac tgattctcta
    15121 cagagttgaa ttctgtgggt ttcgtagctg tttctactcg accgaccaca atcccaacgg
    15181 ctttgacata tgtgtctttg aatgagaaaa ccacatcgcc cggtctcgtt tcggtcatgt
    15241 agtcgtaaaa ttgattgcga gcgccattag tgttggcttt tggagaccac atgtatccgc
    15301 cggagacttc aaattggtaa gtttgatttt gattaaccca ccagtactcc ataagcctcc
    15361 ctttataccc ccctgttgta cctaatcccc catgggactc ttagccgtta gattctatct
    15421 ggcttaggtt ggttaactac ataaggatat ctttttatag ggaaatagaa tctaaatata
    15481 attttatata atcacaatcg ttataatttc ctcactttat cactattttg caattggtaa
    15541 ataggtatcg tgtttcacat caataccggc aggacttacc tttactaatt ggggtataga
    15601 caggccttcc tgtgggggta gcagagttac ccctctgatg acagcccatt cattctgata
    15661 tctacgttct ggatacaatg aattcatctt atagagtcca tctccaaccc tcgtaagaag
    15721 cccctgcttt actaaagcag tcagtcggtt atcaaaatat atctggagag gaatatttat
    15781 cttttctttc cactagagag gttcattact tcgcccagct tcacccattt tcattccatt
    15841 ctcctaatac agcgcttttt cctccgtcca caaataggct ggagcctgta acaaaattag
    15901 actcatcaga tgcaaaataa agtgctgcgt aggcaacatc catggggtga cccatacgtt
    15961 caagaggctg taatttcttt tttttattaa ttaattccgt gtcctctagg tcgtcccaat
    16021 ctattcctcc catttcagtc gcaatggcac cgggttgtac gctgttgacc cttatgttgt
    16081 acttggcata tgagagcgcc gattttttcg tcaacatcgc cattccgcct ttcgacacat
    16141 gataagaagt agatgaagaa gaatttgctc tagtgtctag aacagaagaa ttattgatta
    16201 ttgatcctcc tcccccattc atcatgtggg gcacaacata tttggtgcat aaaaatgctc
    16261 ctttaatatt gatccccatt acttcatcga acacagactc atcttcattg tgtgggtcac
    16321 catcacctga gttatttatg ccggcattat tgaataggac atcgatggtt tcatagtggg
    16381 acattgttct atccaccatg gttataacgt ctttggtaat tgagacgtca gcctgtataa
    16441 atattgcgga tccttcattg tccctgattc tgttaaccac catttcacct ttctctatgc
    16501 gtctacctgc tatggcgact ttggctccct cgctggcgaa cagttctgct gcagcgcgtc
    16561 ccataccaga ggtaccacct gtaatgactg ctacctttcc ttctaatcta tttcttgaca
    16621 ttctttacct gctttaattt tgtgagttag tttcaatatg ggaagcggtg cgattgttgg
    16681 atggtggatt cttagtggtg gcgtattaat acactcaggc ataatacagg aataatggtg
    16741 gagctgagga gactcgaact ccctacctct tcaatgccag ttgtagtgag gaatggtgga
    16801 actcctgtct aacagttcaa aaagcctgcg agtttttcgt aaaagaatca aagttacagt
    16861 tgaaaaatgg gattatcaga gaaaagactt ttgaaaaacg tgagtatgtt atccggcgat
    16921 acatacttcc gttaatggga gatatcaagg tctcacgatt agatgtaaat ctagttgagg
    16981 aaacttacaa gaaaattatc ctctctcaga agaacggtct ggggaaatgt gccagaaccc
    17041 acaagattct aaacaagctt atgaactggc tagcgaggaa aaaaaggggc atcctccaga
    17101 atccgatacc gacaggtcta atgggggatt tgaaaaggga atacaagaac ctagctgaag
    17161 acaagccatc acttaattta aagttgagcg atattcacag aattcttgat cactccaaag
    17221 gtcgaaagca agaggtgatc gtccatttac agtcgcttca tgggttgaga attagtgaag
    17281 cactcgccat acgttatgaa gatatagatt ttataaataa taagatcatc gttagaaatc
    17341 aaacaagcaa aggcaggctg gttccagtta agactaaaag tgcccgacgc agtatcccac
    17401 tccaagaagt aactagagaa attcttgaaa actccccgaa tgcaatgact ggtgggtttg
    17461 tatgcaagga tagcaatggc ggaccgttgc gatactctaa ttatttaaac agattcttta
    17521 cgccaatgat caaagaactc ggactcagat tcacgaccca caacttacga accttcttcg
    17581 ctagttggtg gctggttgaa ggcaaggtag acgtggtaac agtgtcgaaa tggatgggcc
    17641 actccagccc tacggttact atggatatat acgcgaaaac catcgaagag agtgagaata
    17701 gatacgacaa tcggataggt aatgcagtaa tgccccccac cagggctgct tagctagtat
    17761 ggacaacaat ccattacaga tgcggcaaaa aatacggcaa atcctaaccg accttcaatt
    17821 cagagttcac caacacttat tggctatagg ttcaaaaacg gttaaaatgg ttgctcgtaa
    17881 gcgggagtaa ctcagtggct agagtatctg ccttccaagc agaaggtcgc cggttcgaat
    17941 ccggtctccc gctccaacct tgttcaaaaa cactccaatt caaccaacca ttatttcagg
    18001 ctccacatga ctgcgactaa ggaagtaggt ttcacagtag aattttggc
//
LOCUS       DASCNS010000022         3307 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000191359, whole genome shotgun sequence.
ACCESSION   DASCNS010000022 DASCNS010000000
VERSION     DASCNS010000022.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000191359"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            116..1333
                     /locus_tag="QF838_00800"
     CDS             116..1333
                     /locus_tag="QF838_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59266.1"
                     /translation="MDHSTSDKFTELHSKMNLRDIFSVGSSGLRTRRVRAALSVTGIT
                     IGIAALVGILGLSQSGSADLLKDLDALGTNLLTVQAGDGFRSQEATLSLDATAMIDRI
                     GPVYQVSATTKITGGVFLNDLIQDGRTKGITIVAADLNLLSAQRGSLKSGKFLDEMTS
                     QFPFVVLGSVAAERLGIRNTSGDQRVWLKEKWFMVIGIMEPLPLAADLDRSAIIGYPA
                     ADKFLGLDEPPEVIYVRAYPEHIYDVRSVMPATVNPENPEEVQVGRASDVLEARIAAS
                     DTFLNLFVGLGAVALLVGTIGIANVMVIAVIERRNEIGLRRALGATKFHIASQFLTES
                     LLLSVLGGITGVAAGMSVTATYAISQDWSIVIPVYVVVGGILCSILIGGIAGFYPAMR
                     AANMSPTEALRTK"
     gene            1647..2897
                     /locus_tag="QF838_00805"
                     /pseudo
     CDS             1647..2897
                     /locus_tag="QF838_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            2894..>3307
                     /locus_tag="QF838_00810"
     CDS             2894..>3307
                     /locus_tag="QF838_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59267.1"
                     /translation="MIEQTFQTPPERTGGALEGITVIELCGARGHFMGKLMGDMGARV
                     IKIEPLGGAMERQIAPFMHDDPHPDRSLFFWAHNTSKESLEINIEAPEGQEVIKKLTA
                     TADVLLEDFEPGYLPSIGLSYEDLSYGNERLIMTSL"
BASE COUNT          968 a          643 c          802 g          894 t
ORIGIN      
        1 tggaacgact gtaattttaa ttacgcatga tttagaagtg gcagaatcag cacgacgaaa
       61 aatcactctt cgtgatggcc gtgtctattc tgatttgagt cggtgaccta atcatatgga
      121 tcattctact agcgataaat ttactgaact acatagcaag atgaatttga gggatatctt
      181 tagtgtcgga agttctgggc ttaggacgcg gagggtcaga gcggccttgt cagtgacagg
      241 tatcacgatt gggatcgcag ctctagttgg tatattggga ctatcgcaat caggaagtgc
      301 agatttattg aaggatcttg atgctttagg gacaaacctt ctaacagtac aggcaggtga
      361 tggctttaga tcccaagaag ccaccttatc gttagacgca acagctatga ttgaccgtat
      421 aggccctgtc tatcaagttt ctgcaacaac gaaaattacc gggggtgttt tcttaaatga
      481 tttaattcaa gacgggagaa caaaagggat tactatagtc gccgctgatc ttaacctact
      541 tagtgcccaa agaggttctt tgaaatctgg gaagtttctt gatgaaatga catcccagtt
      601 tcccttcgta gtactaggtt cggttgctgc cgaaaggctt gggataagaa atacatccgg
      661 cgatcaaagg gtttggttaa aggaaaaatg gtttatggtc atcggtatca tggaaccatt
      721 gcctctagca gctgatttag acagatcagc gatcataggt tatccggcgg cggataaatt
      781 tttgggcctt gatgaacctc cagaggtcat ctacgttcgg gcataccccg aacacatcta
      841 tgacgtacgc tctgtcatgc ctgcgacagt aaatcctgaa aaccccgaag aagttcaggt
      901 tggacgtgcc tctgatgttt tagaagcgag gattgcagct tcagacactt ttttgaacct
      961 tttcgttggc ctcggggccg tagcactttt ggtaggcaca attggtattg cgaatgtcat
     1021 ggtgatagcg gtgattgaac gccgtaatga aatagggtta cgtcgggctt taggggcgac
     1081 aaaatttcat atcgcgagcc aatttctgac tgaatcacta ctattgtcag ttttaggcgg
     1141 aattacagga gtagctgcag gtatgtcagt tactgcaact tatgccattt ctcaagattg
     1201 gtctattgtc atccctgttt atgttgtagt aggggggatt ttatgctcta ttttgatcgg
     1261 cggcatagcg ggattttacc ctgcgatgcg ggcagcaaac atgtctccga cagaagcgct
     1321 gcggactaaa taaagaggta tcttattatc ttctgcgggg agccaatggt tacaatttct
     1381 attacctcca ctttgaaaaa gagtcctctt ggcttggttt aattacagta aaaggcttaa
     1441 tctatcaata ttcttttcaa ggaatcctag cgaaacaccc ctaatacaat tatcaactca
     1501 actagcagat gttagggctc atacgggtaa ttcaaaccat catcccttgg tattcatgtg
     1561 ttgatacagt taaataaatt cactaccatg atatttgttg gaaaatgtac gaatttaaga
     1621 ttaatgaacc agagtagaga gtttctatgc cggaaccgca aaccttatta agaaaaggcg
     1681 cccttgaggg ggtgagagtt attgattttt cttggattgt tgcaggccct caatgtacca
     1741 ggatactggg agactttgga gcagacgtca taaagataga aaatgacgca aacatggatt
     1801 atattcgagc tagtagaccc gtgggagggt caagatcgcc caaccgtagc ccactgttta
     1861 caacactgaa ccgtaataag agaagtctca ctcttaatgt tatgcacccg agaggaatgg
     1921 agttgataaa agatcttata aaaaaatcag atgtaatagt cgaaaacttc agttccagag
     1981 tgctagagaa gtggggattg ggatacgaag agcaaaaaaa gataaatccg aaaataattt
     2041 actgcagtat gtcggggttt ggccacagtg gtcgagatcg tgattttgtg acttggggcc
     2101 cgacagccca ggccatctca ggattgactt atatgtctgg cctgccaggg gaagaatcgg
     2161 ccggatgggg tttttcatat atggatcaca cgggaggatt ttacggaacc attgcgataa
     2221 tgatggctct aattcaccgc aataaaacag gtaaaggtca gcaccttgat ctttctcaag
     2281 tcgaaagcgg tataggtctt acggggactg cgattcttga ttacacagtg aataatcgac
     2341 ctttccgaag agatgaaatg ccaccaggta atagggctcc agagcgaggt attgctcccc
     2401 acaacaccta tccttgtaaa ggtgaagata gatggtgcgt tattgccgta acttcagaag
     2461 atgaatgggg aaattttgta aaagctgtcg gaaatccttc ctgaacaacc gaagaacgct
     2521 ttgaaaacat gcgatctaga ttcgaaaatc aagattctct agatgaattg atagctcaat
     2581 ggacgatcaa ccatactccc catgaagtct tcgaaattct acaagatgct ggtgttcctg
     2641 caggtgccgt ccagactcca atggaaagag cggaaaaaga cccgcaattg aaacatcgaa
     2701 acttcattgc tgaaattccc cacgaagagc ttggtaaagc gaaggtggaa tccatcccta
     2761 tgcggatgtc ggagactcct tggaagcttg agagaccttc ccctttgctt ggtgaacatt
     2821 ccgccgagat atacacagaa ctactggatg tatctccaga agaattgggt actttgtacg
     2881 aagaagggat agcatgattg aacaaacatt tcaaacacct cctgaaagaa ccggaggcgc
     2941 attggaaggt ataacagtca tagaattgtg tggcgctcgg gggcatttta tggggaaact
     3001 tatgggtgat atgggtgcca gggtcataaa aatagaaccc ctaggagggg ctatggaacg
     3061 tcaaatagcc ccatttatgc atgacgatcc tcatcccgac cgtagtttat ttttctgggc
     3121 tcataatacc tcaaaagaaa gtttggaaat aaacatcgaa gcacctgaag gtcaggaagt
     3181 gatcaaaaaa ctaactgcaa ccgcagacgt attactggaa gattttgaac cagggtattt
     3241 gccttccatt ggcttgagtt atgaagattt gtcatatgga aacgaaagat tgattatgac
     3301 ttcattg
//
LOCUS       DASCNS010000023         2551 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000201155, whole genome shotgun sequence.
ACCESSION   DASCNS010000023 DASCNS010000000
VERSION     DASCNS010000023.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000201155"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="QF838_00815"
     CDS             complement(<1..1002)
                     /locus_tag="QF838_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HJO59268.1"
                     /translation="MLEQFKVQPEDQVRVTEDNLRKTVEAVFAKMGLDDSDSALGADV
                     LVKADMRGVDTHGVSNMLRSYVTGYGNGELNSHPNWTITRETPSTANIDGDRGLGIIV
                     TPKAMDIAIEKAKNVGMGVVTIHNSRHLGMASYHALKAVDNDMIGMCMTSCPPGVLPT
                     FGAEPVLGTNPIAIAAPADKEAPFVFDAAMSAVAGNKLGLARRNGTQLLGGWVADHDG
                     SPIMDKVDPPTPGYEGSASSYLLPVGGTRELGSHKGYGMMCMVDILGGILTGGGYGMN
                     PGRPNFGHYVAAYNIEAFMDTKEFKITMDEWINMLNSSKPAPGHDRVMYPGQPEHEAA
                     "
     gene            1229..>2551
                     /locus_tag="QF838_00820"
     CDS             1229..>2551
                     /locus_tag="QF838_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HJO59269.1"
                     /translation="MTLTRPVVINTSMWILIPSLIAAMVCITPLIYLINRVVDSDSSV
                     WAWLMRPKTVMIFTRSIFLAITVTMFSAVIAVPIAWLTIRTNLPYRKLWSVLTFLPLV
                     IPSYVGAYILISFSGNGGLLHEITGTFFDVGSVPTMYGYYGSLVTLSLLNFPYIILTI
                     RSNLSNMDVSGEETAKMMGLSHFQTLIKVTIPSIKPAILSGSLLVTLYTLSDFGAVSL
                     LRYKTFTWSIYTQYESGFDRSTAALLSLTLFIVATGFMFFELIVRGNKKYYKNSSSAS
                     KKLPVIDIGKWRWPAAGLCAVISLLSVAIPILVLLYWVLRGLYHGESAAILSAESNHI
                     LVLNSIMLSVLTAAVVVTLSLPVGYLAIRHSGFLSSTIEKMCYTGYALPSIAVSLALV
                     FLGSKIGSPIYQSIYLLVMACAVLCMPTGLSAIRSSMTQISPRYEESSM"
BASE COUNT          692 a          582 c          487 g          790 t
ORIGIN      
        1 cgctgcctca tgctcgggct ggcccggata cataactcga tcatgaccgg gtgctggttt
       61 agaagaattc agcatattaa tccattcatc catggttatt ttaaattctt ttgtgtccat
      121 aaaggcttcg atgttgtagg cagctacata atgcccaaaa tttggtcggc cagggttcat
      181 cccatacccg cctcctgtca gaatacctcc taatatgtcg accatgcaca tcatcccata
      241 ccctttatgt gagcctaatt ctcgcgtccc tccaacagga agcaaatagg atgaagcaga
      301 accttcatag cctggtgttg gggggtccac tttatccata atagggctac catcatgatc
      361 ggccacccat cctcctaata attgcgtgcc atttcttcta gcaagcccta gtttattgcc
      421 tgcaactgca ctcatggcag catcaaacac aaagggagcc tctttatcag caggagccgc
      481 aatcgctatc ggattcgtac ccaaaacagg ttctgctccg aaggttggta aaaccccagg
      541 agggcacgat gtcatacaca tgcctatcat gtcgttgtcc actgctttca aagcatgata
      601 tgaagccatg ccaagatgcc tcgaattatg aatagtcacg actcccatac ccacattctt
      661 agccttttca atcgctatat ccattgcttt cggagttaca attatcccca agcctctatc
      721 gccatcaata tttgcggtag acggggtttc tcttgttata gtccaattag gatgagaatt
      781 aagttccccg ttcccatacc cggtgacata agaccgcaac atattagaga caccatgagt
      841 gtcaacacct ctcatatctg cttttactaa aacatcagcc cccaatgctg aatcagagtc
      901 gtcaagcccc atttttgcaa atacagcctc caccgttttt ctaaggttat cttcggttac
      961 tctaacttga tcttccggtt gcactttgaa ttgttctaac aatttagtcc cccttcaatg
     1021 ccttctgttt gagagcgcat tctacgttca gtagggaaaa acagcaattc agaagaggaa
     1081 atttaagagt atgttctacg aaactatatt cattagttca tactttctta ttaaattaga
     1141 ctatacttac tataaaattc ttatgattct tcaatcttga catggatact tcaattatcg
     1201 ctataattcc gaggaaaatc agctagcatt gactttaaca agaccagtag ttatcaacac
     1261 ttcaatgtgg attttaatac catcgctaat tgcagctatg gtgtgtataa ccccattaat
     1321 ttatctcata aatcgggttg tggattcaga ctcatctgta tgggcatggc ttatgaggcc
     1381 caaaacggtc atgatcttta cgagatctat attcttagct atcaccgtta ccatgttttc
     1441 ggccgttata gcagtgccaa tcgcgtggtt gacaatccgc actaatttac cataccgcaa
     1501 gctatggtca gttcttacct ttctaccgct cgtgatacct agttatgtcg gagcatacat
     1561 tctcatatcg ttcagcggaa atggaggtct tttacatgaa atcacaggga ctttctttga
     1621 cgttggtagt gtacctacta tgtacgggta ttatggaagc ctagtaactt taagtctact
     1681 caatttcccc tacataatac ttacaattag gtcgaattta tcaaatatgg atgtgtctgg
     1741 ggaagaaact gccaaaatga tgggtttgag tcactttcaa actctaatca aggtaaccat
     1801 accttcaatc aaacctgcga tcctatcagg atcactatta gttactttat atacactgag
     1861 tgattttggt gctgtttctt tacttcgtta caaaactttc acttggtcaa tttatactca
     1921 atatgaatct ggatttgata gaagtacggc cgcattgtta tcgctaacat tgtttattgt
     1981 ggctaccgga tttatgttct ttgaacttat agttagggga aacaaaaaat actacaaaaa
     2041 ttcaagtagc gcatcaaaaa aactccccgt gatagatata ggaaaatggc gatggccggc
     2101 tgccgggttg tgcgctgtga tatctctgct atctgtcgca ataccgatat tagtcctact
     2161 ttattgggta ctcagggggc tgtatcacgg ggagtcagcg gctattttaa gcgcagaatc
     2221 caatcatatt ttagttctga attccatcat gctttctgta ctcacagcag ctgtagtagt
     2281 taccttatcc ttaccggtgg gttatttagc aatcagacat tctggctttt tatcaagtac
     2341 catcgaaaag atgtgttaca caggttatgc attgcccagc atcgcagttt ctttggcact
     2401 cgtttttttg ggatcgaaaa tagggtcccc catctaccaa tcaatatatt tattggtgat
     2461 ggcctgcgct gtcttgtgta tgcctacagg gttgagtgca ataaggtcat ctatgaccca
     2521 aataagccca aggtatgagg agtcatcaat g
//
LOCUS       DASCNS010000024        20173 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000220042, whole genome shotgun sequence.
ACCESSION   DASCNS010000024 DASCNS010000000
VERSION     DASCNS010000024.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 20173)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20173)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20173
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000220042"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(122..1147)
                     /locus_tag="QF838_00825"
     CDS             complement(122..1147)
                     /locus_tag="QF838_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HJO59270.1"
                     /translation="MTSESGALLEIKNLKKHFQVGGGMFGSMTGKKEFLKAVDGISLS
                     IEPGTTYGLVGESGCGKSTTAKMVLMLEEPTEGEILFEGKDAINSDKDAKRYYKSSVQ
                     AVFQDPWASLNPRMKVESIIAEPLEVNTTMTRGEIHTKVGEILEEVGLQQYQAELYPH
                     EFSGGQRQRIGVARSLALNPKLIVLDEPVSALDVSIRAQIMNLLMDLQKEHGLTYLLI
                     AHHLATVRYMCDKMGVMYLGKLVEEASTDQLFDTPTHPYTQALIGAALPYHPDDQKEE
                     MLLEGEVPSPINPPSCCSYAPLCPEVDPLCEANELITREVAPGHHIAHCAHCADEYGC
                     YWFPRER"
     gene            complement(1182..2213)
                     /locus_tag="QF838_00830"
     CDS             complement(1182..2213)
                     /locus_tag="QF838_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO59271.1"
                     /translation="MASDSRIGDVVLEVKDLQTYFVTRWGVTKAVDGVSFNLRKGETL
                     GIVGESGSGKSVTVSSVMRLIPSPPGHIVGGEILLDGENLLELDESDMNKVRGDKIAM
                     VLQDPMTALNPVFDIEDQVGEALKIHEGLKGPTLKETVIDMLRKVKIPAPSTRMKDYP
                     HQLSGGMRQRVVGAIGISCNPSVLIADEPTTSLDATIQAQYLNLLRDLQEDTGVGIIF
                     ITHDFGIVAKMCDRVAVMYAGKIVEQGDVREIFNNPLHPYTEALIRSVPKMEENVERL
                     YAIPGNPPNVTNRPSGCNFQPDCDKDNPNPDCELLEPILQEESPGHWVAYCAACFDTH
                     GCKWFPRDL"
     gene            complement(2396..2716)
                     /locus_tag="QF838_00835"
     CDS             complement(2396..2716)
                     /locus_tag="QF838_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019590.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HJO59272.1"
                     /translation="MIYDMRIYDLKPGSVPEYMSAVEEVALRIRQDYGIKLAGWYYSD
                     IGPLNRIVHIWGYQNYTHFEESRIKVRSDPRWDRDYLPRVKNLILAQQDMIMNGANFF
                     PEPK"
     gene            complement(2770..3159)
                     /locus_tag="QF838_00840"
     CDS             complement(2770..3159)
                     /locus_tag="QF838_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59273.1"
                     /translation="MQKETFLNLGVQEYSSILVFSPPEHHVQEIAQMDLPEGSSVSYV
                     GDGMFDVILIFTRNQRDLSELAAAGVAVLKKTGFLWFAYPSPKSRLATDISPSEGWDI
                     LLDAGWSQKDSFDLDDNWIATKFGEDI"
     gene            3469..3648
                     /locus_tag="QF838_00845"
     CDS             3469..3648
                     /locus_tag="QF838_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59274.1"
                     /translation="MVDYRACPKCRGDMHTRRDTFGDFKECIQCGLMQDLELENKSDA
                     FTVSDGPKNKTGQAA"
     gene            3895..4251
                     /locus_tag="QF838_00850"
     CDS             3895..4251
                     /locus_tag="QF838_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HJO59275.1"
                     /translation="MTTTQGPAPLSITISEEAAYHVKEFSAAEGNPDADLRVGVKGGG
                     CSGLTYMLEIVSDGPRETDKVIDENGVRLFVDKKSYVFLAGTILEYSGGLNGKGFVFN
                     NPNAKTTCGCGTSFSV"
     gene            4264..5163
                     /locus_tag="QF838_00855"
     CDS             4264..5163
                     /locus_tag="QF838_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013720.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59276.1"
                     /translation="MIEKPVFVDLSDYFSRIDIYGEDSLDLLDRLSTNKLDDLTDPFM
                     GMHSVLTTNKGRIIDLLSVNRLPDKVLLMTAGESKNKVIDWIEFYTIMEDVTVKDVST
                     ETCHFRLIGDSWEDLFPRMTDLSIRNSFIWEFEGESFIAIKEEMSSFPCVDIIGTAGS
                     KNKLNQVFSAECKSLTLEEFTQLRLELGIPAFGYELTEDFNPLEAGLISHINFNKGCY
                     IGQEVVARLNTYDKVQRKLVRLSWEGILAEKDITYDGHLVGVITSAMNGAGLGFIRNS
                     HAELSRELVCGNQKVMVTEILAK"
     gene            complement(5164..5934)
                     /locus_tag="QF838_00860"
     CDS             complement(5164..5934)
                     /locus_tag="QF838_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59277.1"
                     /translation="MADTKNKVALITGGSRGIGKAIAMEFGNLGYHIAFNYFRNHDAA
                     EQTQLEIENLGVRCLKLRAHLAEPDQIANMFQSVEQEFSSIDVLVNNAASGVQRNADE
                     LTTKHWDWTMNINARAPWLCSVEATKIMPNGGSIVNITSEGSRKVLPYYLSVGTSKAA
                     LESLTRYLAVELAPKGINVNAVSGGYVETGAFESFPNKQDMVTSAKNTLAGRVITGKD
                     IAKVTAFLCSEDALMVRGQIIIVDGGVTLLAPLSSTDS"
     gene            complement(5958..6704)
                     /gene="fabG"
                     /locus_tag="QF838_00865"
     CDS             complement(5958..6704)
                     /gene="fabG"
                     /locus_tag="QF838_00865"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HJO59278.1"
                     /translation="MSELKGKTALVTGAAKGIGRAAAIRLAEMGANVVVNYNSSQKEA
                     DDTANHLEDFGVESISYQADVGNLSEVTKMVEDVTEQFGQIDILINNAGIIDDGLLLR
                     MSDEAWSRVINTNLNGTFYCSRAVLRTMVRSRWGRIINIGSVVGLRGNIGQTNYTASK
                     AAINGFTFALAKEVATRNITVNTVTPGYIHTDTVDVLSDKQKDTIMTWIPMAHFGEVD
                     DIAHMIAYLASEKAKYITGQIISVDGGMAI"
     gene            complement(6742..7764)
                     /gene="plsX"
                     /locus_tag="QF838_00870"
     CDS             complement(6742..7764)
                     /gene="plsX"
                     /locus_tag="QF838_00870"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HJO59279.1"
                     /translation="MPETQDRLLKVAIDAMGGDNAPSLPVAGAVQAAKSGLAEVFIVG
                     DSDLVQNELAKHETVGLPVHFVPSNGKIEDHEPPALALRQKPTASILVATNLVKNKTA
                     DAVISMGSTGGTMAAAVVLLGTIKGIERPAIGGPIVGSAPNQTILDLGSNLDCKPSQL
                     LGFGVLGSVFSKLLIGVKDPKLGLLSVGSEEGKGNVQSKEASMLFKKSGLNFIGNIEG
                     NDLVTGKADVVVCDGFIGNVVLKLVEGLGRGLATQLSEVLKGKIPDDHLDSLMADLYK
                     KNNVVDARGGGPVFGVNGVSIIGHGNASAAAIERAVGLAKMCVNTNLIEEMNKEVSLV
                     MSTVDD"
     gene            complement(7775..8194)
                     /locus_tag="QF838_00875"
     CDS             complement(7775..8194)
                     /locus_tag="QF838_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024844.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJO59280.1"
                     /translation="MKLGADLIPIKKMITMDQIRMYADASGDYNPIHINEAFGKNSQF
                     GRNIGHGMMAAATISEIMAESFGTAWYNTGKMKMRFRSPVYPGDEIQTSGQIKKLEDT
                     TNSSHITCTVIITRQDGEVIISGEASVKVDKPASAGD"
     gene            complement(8215..8619)
                     /locus_tag="QF838_00880"
     CDS             complement(8215..8619)
                     /locus_tag="QF838_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024844.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJO59281.1"
                     /translation="MPLDYSLLESGNEISNQTYTLGQKEVALYLEAVQDKSSREFDKS
                     GIELSPPMAVAALSLRGVVVDLQIPGGTLHVGQEMEFLNSVKVGETLRCVASLASNNV
                     RGEWRFMVVSLIVLNSSGLSVMKGKSTIMLPA"
     gene            8847..10112
                     /gene="serS"
                     /locus_tag="QF838_00885"
     CDS             8847..10112
                     /gene="serS"
                     /locus_tag="QF838_00885"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HJO59282.1"
                     /translation="MIDIELIREKPEFVIQSFKRRGKELPLTEISDLDQRRRAAITEI
                     DSLRSRRNAVSKDLGQSKDKPAELIEEMRNVGSQIKDLEEEERTISGRLNQVMLDVPN
                     LPSESSPDGPDENSNIVIRTVGSIPKFDFNPKPHWEIGENLGIIDFQRGVKLAKTRFY
                     LLKGQGARLQRALISWMLDLHTEKHGYSELYLPYMVNRDTVTASSHLPHFEENMYHDA
                     EDDLFMVPTAEAPVTGMFRDEILDAETLPLKFVSHTPCWRREKFSAGRDTRGIKRVHQ
                     FDKVEMYKFVEPDKSYEELELLLNDAEAVCVELGISHRILELCTGDLGFAAAKSYDVE
                     MWSPGTQEWLEVSSCSNCTDFQSRRAAIRYKASENSRPRLLHTLNGSGLAVPRVMIAI
                     LENYQLPDGTVKIPDVLKPYAGFDLITGD"
     gene            10156..10530
                     /locus_tag="QF838_00890"
     CDS             10156..10530
                     /locus_tag="QF838_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014376.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJO59283.1"
                     /translation="MYVTRQEDVDPQEAGDAPLFYGGRVTRQPLVGRTEPEDYNFGLV
                     SFYAGAKNYFHTHTSDQILFATKGQGIVANESEEVQMGAGDTAFIPAGEKHWHGAADG
                     HDFSHISLTRPGSVTEMFKPES"
     gene            complement(10533..11516)
                     /locus_tag="QF838_00895"
     CDS             complement(10533..11516)
                     /locus_tag="QF838_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO59284.1"
                     /translation="MLKISPAVIDHLSVTVLMDNHISSLLSDTGPVKRPSTEVEVAAP
                     LTVDGFVKSQLRAEHGFSAWVTIQNNSNQHSVLFDAGVSPMGVSENMRILGISPKDAE
                     AIVFSHGHFDHTMGIDGILNDLGSQNTPIIIHPEFWNKRRIRLPGKTPRILPTASKFA
                     LQEGGLEVIEESRLPSFLLNNSLLITGEVDRVTSYEKGLAGQEVFTEGSWYPDPLTLD
                     DQALIAKIRGKGLIIMTGCGHSGIVNICNYAKKLTAEDKIHAIIGGFHLPDGLDTATI
                     QNTISDLKSLDPDWLVPAHCTGQKAILSLVKELPNSIIQNSVGSQYVFSSF"
     gene            11694..12674
                     /locus_tag="QF838_00900"
     CDS             11694..12674
                     /locus_tag="QF838_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO59285.1"
                     /translation="MFAARITSPKKIEIEDIETPTIKEGQCLIKLERWSVCGSDIRHA
                     YGPVMPEEEYPMRHGGACHECAGTIVESKSDKFKVGQRVIVLPSQDGPGGLVEYYPGD
                     ESRMALVPDHGDLGEWILCQPSGTVLYSCQQMGTILGKDVVVMGQGSIGLSFTAIVAR
                     AGARRVIAVDPLDYRLEWGKKFGATHTINPDRDNVDEALAEITDGKLADISVEASGYP
                     DGLNAAIRGATKHGKVIIFGIQEGPRGKTTEIDTQYLLRNAPTIIPTSGAGSGDAIGH
                     IERMIELKDRGWWNPGEMKTHETNFSDVNDAYDQYEQYQDDIIKVVMNNE"
     gene            12770..13933
                     /gene="dgoD"
                     /locus_tag="QF838_00905"
     CDS             12770..13933
                     /gene="dgoD"
                     /locus_tag="QF838_00905"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HJO59286.1"
                     /translation="MKITSVKAVPASHTTTRAGVSVSRNYVFVKIETDEGLTGWGEAT
                     CGPLSVATMVDEFGEVINGQNPMEIEKHWQTLYHHFHVRGGIVQMSAISGIEIALWDI
                     KGQALGVPVYELLGGKIRDRIWTYGRWDGLTPEAAVENAMFHVEQGLTALKGDPFEHR
                     GIFIDKKSEDLAVSKMRSVREAVGDEVELFVEVHGRLAPSDAIRVANRLEEFRPFFYE
                     EPVPPQNLEALKKVADNVNIPIATGERLLTKFDFADLLPLHAVDLIQPDVVQAGGILE
                     LKKIAAMAEAYYVGFQPHNPYGPFCTIAALHLDACTPNFLIQEGGIHPWFQDATTGNF
                     PIQKNGYFGVPTGIGLGVAMNEDWLKANPWSDNAYPWRPNLGANPSRQDVFWS"
     gene            14033..14353
                     /locus_tag="QF838_00910"
     CDS             14033..14353
                     /locus_tag="QF838_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59287.1"
                     /translation="MTEREKMTMVSQLRIYTINRGMMDSWLNLFHKHLKPNHEKYGIP
                     IESSWVNADRTEFIWVRSFDNVEVIQAKEDEYYSSPERIALGDQPKVHIAKTEVRVVE
                     PTNS"
     gene            14421..16391
                     /locus_tag="QF838_00915"
     CDS             14421..16391
                     /locus_tag="QF838_00915"
                     /inference="COORDINATES: protein motif:HMM:NF040438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HJO59288.1"
                     /translation="MAVTAVDIKSRVEFDSGTSWGKFGPYERIDGVVEFGVDPDNAAN
                     SGIIDLHHSPVDSAGLVNFSSDFVLLTPSTRESSRLLVDVVNRGRKRAISDFNMASPN
                     LTPSSTIEPGDGFLFQRGYTVVSIGWQYDVYRSGSLLGMDPPLVELDGKPVEGTNLVE
                     IRPNERIKNSLLANRAHKPYPASSTNNAKDTMYVKDWEDGPQEEIPRSEWSFSKEQNG
                     TVVPDAEYVYMESGFCPGRIYSVVYEASKAVVSGSTLMSIRDIGSWIKYGGVNSPLKS
                     RPTYAYAYGISQTGRLLRHYLYSGMNLDEKGRQVYDGLLPHVAGGRRGDFNHRFAQPS
                     QQSSPGFGHMYPFADEPTEDPYGRKLEGLQDRQKTLGGLPKVIYTNTSAEYWRGDASL
                     SHIDLRGRQDLDFPQNTRSYLFSGTQHVPRELPQMKDPGPDGSLGLYGFNVVDFRPLL
                     RSALCNLVSWVEEGLEPPESKVPRLADGTASTIPDVLEVFTGTLGLKIPDHSQMWRLR
                     EMDMGLREDIGIATYPIKEGREYPRFVSSVDNDGNEVAGIRMPDISVPVGTHTGWNPR
                     DPSTGAPDQIISMVGFTNYFPATGKSFRPHDDLRNSNNDRYSSKENYLKMVSKAAEKL
                     VEERYLIKEDIDVVLQKCAQRYGEAISRGNQV"
     gene            complement(16447..17406)
                     /locus_tag="QF838_00920"
     CDS             complement(16447..17406)
                     /locus_tag="QF838_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HJO59289.1"
                     /translation="MNINSTVRNWEKDTTPYPEPFVEVIDESFKRYVLGNAGMERLYT
                     GSRWTEGPAWFGDGRYLLFSDIPNNRILRWTEETEIVSEYRNPSNNANGNARDNLGRL
                     ITCEHLTRRVTRTEFDGSITIIADSFKNSKLNAPNDVAIHKDGAIWFTDPGYGILNNY
                     EGDRSEPELPTAVYRIDPSSGAVAVKATGLVRPNGLCFSPDFTDLYVSDSGSNNPNAI
                     YVYQVSNNGDSLAEGKIFTETGEDSTDGMRCDTDGNLWASAQRGTNSITIYNDSSVLI
                     GRIYLPEVPSNLTFGGHKRNRLFITASRSIYSIYTDATGASLS"
     gene            17646..18866
                     /locus_tag="QF838_00925"
     CDS             17646..18866
                     /locus_tag="QF838_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59290.1"
                     /translation="MCSSGVRSSMGVFITPMETDMQWARNDITRVLSLGIFVGALSFI
                     LMGYLYDRYGGRRVITAALIMVGISVFLLSRVNSIFGLILIYGILGSVATSGASFVTI
                     HSLLAKWFYRRRGLAMSLSAAGGSIGPLLFAPFCAFLIEAFDWRTAFLFIGGMVLFIG
                     APLAALFLKDDPLGQIPDSERDETGVQDKEEVQVKMEGPLFTSRWKQALSTSPFWQLS
                     AAYLVCGITTNIISVHFVPFAEDQGVPKITAATIFGIMMGLNSVGVISAGVLSQKFSQ
                     HRVLGFTYALRGIAYLALLTIGGTTGMWAFALIAGMSWIATASVTSSLVADIYGVRNL
                     GTLNGMSNMVHQIGGALSVLVAGELQAWTGSYEIPFAIAGATLLFASIMAFSVNEKKY
                     SYRYSRVPVAAAPS"
     gene            complement(19246..19524)
                     /locus_tag="QF838_00930"
     CDS             complement(19246..19524)
                     /locus_tag="QF838_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59291.1"
                     /translation="MTDLKTLDQLYTHIMKTIVSTGVAPHYTEIATSLGVEVEQARTS
                     LNDLMGPGKMAGYWLVPGTDTIGSFAPFNNIPTHYKITVDGESKWWGQ"
     gene            19747..>20173
                     /locus_tag="QF838_00935"
     CDS             19747..>20173
                     /locus_tag="QF838_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59292.1"
                     /translation="MAIASAVSMGMGSLNFGLYIKPMGEQLGIGRASFGWSSTFRSIS
                     SAATSPIIGRLLDRHGARWILAGSTIITGLCMVGLAYTREAWQLMVLFSIMGLVGMSG
                     PGALTTSVPPSKWFVRNRGKALAVTAVGVSLGALIFVPLT"
BASE COUNT         5570 a         4076 c         4464 g         6063 t
ORIGIN      
        1 caatacaaca aaaatcgcaa gtgaggaacg gaaaatactg gttgatagtt ccagtctagg
       61 gtataaattt tgcggcgcaa acagataaat taagctttgc gccgcaaaaa atgtctatca
      121 attatctttc ccttggaaac caatagcagc cgtattcgtc tgcacagtgg gcgcaatgag
      181 ctatgtggtg gccgggagcc acttctctgg taatgagttc attcgcttca catagagggt
      241 ctacttcagg gcataaggga gcgtagctac agcatgatgg cgggttaatt ggtgaaggaa
      301 cttctccttc caataacatt tcttcttttt gatcatcagg atgatacgga agggccgcac
      361 caattagggc ttgggtatat ggatgagttg gggtgtcgaa tagctgatcg gttgaagcct
      421 cttcaactaa ttttcctaag tacataacgc ccattttgtc acacatgtat cttacggtag
      481 ccaaatggtg ggcaattaga aggtaagtga gcccgtgttc tttttgtaga tccataagca
      541 ggttcattat ttgggcccta atagaaacat ccaaggctga aactggctca tccaacacaa
      601 taagttttgg atttaatgca agggatcttg caacaccgat cctttgtctt tgcccaccgg
      661 aaaactcgtg agggtatagt tcagcctggt attgctgtag cccgacttct tccaagattt
      721 ctcctacctt ggtgtggatc tcacctctgg tcattgtcgt atttacttcg agaggttcgg
      781 cgataatact ctctactttc attcgtggat tcaaagaggc ccaagggtct tgaaacacag
      841 cctgcacact ggatttatag taccttttgg catctttgtc cgagtttata gcgtcctttc
      901 cttcaaatag tatttcccct tcggtcggtt cttctagcat gagaaccatt ttcgctgttg
      961 tggatttacc acacccagac tctccgacca gtccataggt ggtgccgggc tctatgctta
     1021 aacttatacc atctacggct tttaggaatt ctttttttcc tgtcatagaa ccaaacattc
     1081 cacccccgac ctggaaatgt ttcttgagat tcttaatctc tagcagggct ccactttcac
     1141 ttgtcatatt caacctcttt ccatttattc aaactcagat tttataagtc tctcgggaac
     1201 catttgcagc catgtgtatc aaaacatgct gcacagtagg ctacccaatg tcctggagac
     1261 tcttcctgaa gaatcggctc cagcagttca caatctggat tagggttatc cttatcacag
     1321 tctggctgaa aattgcaccc tgaaggtcga tttgtcacgt ttggtgggtt tccaggtata
     1381 gcgtatagcc tttcaacatt ctcttccatt tttggaactg acctgatcaa ggcttctgta
     1441 taaggatgca gagggttgtt gaaaatttcc ctaacatctc cctgctctac aattttccca
     1501 gcatacatca cggcaaccct gtcacacatt tttgcaacta tgccaaagtc gtgtgtaatg
     1561 aaaataattc cgacccctgt atcttcttgg agatctctca gaagattcaa atattgtgcc
     1621 tgtattgtgg catccagaga agttgttggt tcgtcagcaa tcaacactga agggttacaa
     1681 gatataccaa ttgcgccaac gacacgctgc ctcattccgc ctgacagctg atgcgggtag
     1741 tctttcatcc tggtgcttgg tgcgggtatt ttcacttttc ttagcatgtc tataactgtt
     1801 tctttcaggg tgggcccttt aaggccttcg tgaattttaa gagcttcacc gacctgatcc
     1861 tctatatcga aaactggatt caaagcggtc atagggtctt gcagaaccat cgctattttg
     1921 tcacccctca ccttgttcat atcactttcg tctaattcaa gaaggttttc cccatccaag
     1981 agtatttcgc caccgacaat atgaccaggt ggagacggta ttagtctcat tacggaactt
     2041 acggtcacgg attttcctga tcctgattct cctacgatcc ctaatgtttc cccttttcga
     2101 aggttaaaac taactccgtc aactgccttc gtaacacccc aacgtgtcac gaaataagtt
     2161 tgaagatctt tgacctccag tactacatcg ccaattcggc tatctgaagc catgacttga
     2221 actcctatct tcctggtttc ttggtgtttt caaaagagta aaaatttctt gcgaaaacag
     2281 cgcctaattt caggcacacc tatacaaatt aagtacccgc cactataacg aaagggtcag
     2341 gaaaaggcaa taggtccaca ggcatgaatg gggggggggt tgagactaag gggaactatt
     2401 ttggttcagg aaaaaaattc gctccattca taatcatatc ttgttgagcg agaattaaat
     2461 ttttgactcg tggtagataa tctcggtccc accttggatc agaacgcacc tttattcggg
     2521 attcctcaaa atgggtgtag ttttgatacc cccaaatgtg tacgatcctg ttgaggggcc
     2581 ctatgtcgct gtaataccaa ccggctagtt taataccata atcttgccga attcttaatg
     2641 ccacttcttc taccgcgctc atgtattctg ggactgatcc aggcttcaga tcatatattc
     2701 tcatgtcata tatcaaagct tcctccacat catagtttat gtttcaggtc ttctatcaat
     2761 tgcctactat taaatatctt caccaaattt tgtagcgatc caattatcat caaggtcaaa
     2821 ggaatccttt tgggaccagc ctgcatcaag aagaatatcc catccttcag agggagaaat
     2881 atctgttgct aagcgggatt ttggtgacgg gtaggcaaac cataaaaatc ccgttttttt
     2941 aagaacagcc actccagccg ctgctaattc tgatagatcc ctttggttgc gggtgaatat
     3001 taaaatcaca tcgaacatac cgtcgccgac gtatgaaact gaggagcctt cagggagatc
     3061 catctgagct atttcttgga cgtggtgctc aggaggcgaa aatacaagta tggaagaata
     3121 ttcttggaca ccgagattga gaaaagtttc cttttgcata aataagaatt tccttcattt
     3181 aacctctcta aaaggaatct gactaggtaa caatatcata aaatgcgata ctaaacatat
     3241 aattatccac tcttgcaata ttcagtgata tcaatataat tccgctttgg cttcttatgc
     3301 ttggtaacgc gcgagacgaa ctaactgcac tttatctgaa taagtgagtc gaaaaattat
     3361 tgagtaacag aaagagtgac gattaaaaaa atggaagaag ttccgttgat aggtttcatg
     3421 aaatttaaac aaaatgtttt ggaaattgag aatgtgggga attgaaaaat ggtagattat
     3481 agagcgtgcc caaaatgccg tggtgatatg cacacccgcc gagatacctt cggggatttt
     3541 aaggagtgta tccagtgtgg cttaatgcaa gatcttgaac ttgaaaataa atcagatgcc
     3601 tttacggtat ctgatggccc taaaaataaa acaggacaag ctgcttaaag tataggattt
     3661 aatcagtatt tcaggtactg ttaaagcaat cttcacaaat atagaatagt tgaatgtctg
     3721 gtggttcgtt gtgcctaaga catatcgata tgacgtagaa ggcggcttct tccatattga
     3781 ctgactacca ctatatttat agaatcaatt atctaaaagc gttttggaat ggttacgcac
     3841 cattgagtac agtggaaatt agactatgat acatgtagat gatgggaggt ctaaatgaca
     3901 acgactcagg gtccagctcc actttctatt acgataagtg aggaggctgc ttaccacgtt
     3961 aaagaatttt ctgctgctga gggaaaccca gatgctgatt tgcgcgtggg ggtaaaaggt
     4021 ggcggttgct caggactcac ctatatgttg gaaatagtga gtgatggccc aagagaaacc
     4081 gataaagtga tcgatgagaa tggtgttcgg ctttttgttg ataagaagtc gtatgtcttt
     4141 ttagcgggta cgatactcga atattctggg ggacttaacg gaaaggggtt tgtattcaat
     4201 aaccccaatg caaaaacaac ctgtggttgt ggcacttcgt ttagtgttta ggtaacactt
     4261 tatttgattg aaaaaccagt gtttgtagat ctaagtgatt atttcagccg gatagatata
     4321 tacggggagg attcactaga tctcctagat agattgtcca ccaataaact tgatgatctc
     4381 actgacccat ttatgggaat gcactctgtc cttaccacca ataaagggcg aataattgat
     4441 cttctcagtg tgaaccggtt acctgacaaa gttctattga tgacggctgg tgaatctaaa
     4501 aacaaagtca tcgattggat agaattttac acaattatgg aagacgttac agtcaaagat
     4561 gtttcaacag aaacctgtca ctttaggttg atcggtgata gctgggagga tctatttcca
     4621 agaatgactg atctatccat ccgcaattct ttcatttggg aatttgaagg agaatctttt
     4681 attgctataa aagaagagat gtcttctttt ccatgtgttg acattatagg tacagcgggt
     4741 tcaaaaaata aattaaacca ggtattttct gccgagtgca agagtcttac tctagaggaa
     4801 ttcacacaac tgagattgga gcttggaatt ccagcgtttg ggtacgaatt aacagaagat
     4861 tttaatccgc tggaggcagg cctaatatcc catattaact ttaacaaagg gtgttatata
     4921 gggcaggaag tggtagctag attaaacact tatgacaaag ttcaacgtaa gttagttaga
     4981 ttgtcttggg aaggtattct agctgaaaaa gacatcacct acgatggtca tttggtggga
     5041 gtcataacct cggcaatgaa tggcgctgga ttaggattca ttcgtaactc tcatgcggaa
     5101 ctgagtagag agttagtatg cggcaatcaa aaggttatgg ttaccgaaat attggcaaaa
     5161 tagttatgaa tcggtgcttg atagaggtgc tagtaaggta acaccaccat caactattat
     5221 gatctgacct cttaccatca aagcgtcctc actacataaa aaagcggtga ctttggcaat
     5281 gtctttacct gttattaccc ttcctgcaag ggtgtttttc gccgatgtca ccatatcttg
     5341 cttatttggg aatgactcaa aggcaccagt ttccacatat cctcctgata ccgcattgac
     5401 attaatacct tttggagcca attctacagc taggtatctg gttagagatt ctagagctgc
     5461 ttttgaggtc cccacagaga ggtaatatgg aagaaccttt cgggagcctt cagaagtgat
     5521 gtttactata gaaccgccat ttggcattat tttggtggcc tctaccgaac acagccacgg
     5581 ggcccgagca tttatattca ttgtccagtc ccagtgtttt gtcgttaatt cgtctgcatt
     5641 tctctgaaca ccagaggctg cattatttac taagacatca atagaactga attcttgctc
     5701 aactgattga aacatgtttg caatttgatc aggttcagca aggtgggctc gcaatttaag
     5761 gcatcttact cccaggtttt ctatttcgag ttgagtttgt tcggcagcgt cgtggtttct
     5821 gaaataatta aatgcaatgt ggtaccccaa gttgccaaat tccatggcaa tagctttacc
     5881 tatacctctc gaaccgcctg ttatcagggc taccttgttt ttagtatcag ccattaaatt
     5941 attctaggct ctgttcgtta gatagccata cccccgtcaa cgcttattat ttgacccgtt
     6001 atgtattttg ccttttcact tgctaggtat gcaatcatat gagcgatatc atctacctcc
     6061 ccaaaatgag ccattggtat ccaagtcata atggtgtctt tttgtttgtc tgataggaca
     6121 tcgacagtat cagtatggat gtaccctggg gttaccgtgt taacggttat atttctcgta
     6181 gctacttcct ttgcgagggc gaatgtaaaa ccgttaattg cggcttttga agcagtgtag
     6241 ttggtttgac caatgtttcc tcgtagaccg actaccgaac caatattaat gattctcccc
     6301 caccgggacc tcaccatcgt tctaaggacg gccctgctgc agtaaaacgt accgttgaga
     6361 ttggtattta ttactctaga ccatgcctca tctgacattc ttaatagcaa cccatcgtcg
     6421 ataatgccag cgttatttat taatatgtcg atctggccaa actgttcagt tacatcttct
     6481 accattttag ttacttcact caaatttccg acatccgctt gatacgaaat ggattccacc
     6541 ccgaaatcct ccaaatggtt agcagtgtca tcggcttctt tttgggaaga attataattc
     6601 actacaacgt tagcccccat ttcagccaac cttattgcgg cggccctgcc gatccctttg
     6661 gctgcccctg taactagggc tgtttttcct ttgagttcag acacttttac tcctataaac
     6721 ttatgccagt aaccaattta tttaatcatc cacagtggac ataaccaagg atacttcttt
     6781 attcatttct tcaattagat ttgtgttcac acacattttt gccagcccca cagctctttc
     6841 tatggctgca gccgaagcgt taccgtgtcc gattatactc acgccattga ctccgaaaac
     6901 tgggccacca cctcgtgcat caactacgtt gttcttttta tatagatctg ccatgagaga
     6961 atcgagatga tcatcgggta tttttccctt caagacctca cttaactgtg ttgctaatcc
     7021 ccttccaagt ccttctacca atttcagaac gacatttccg atgaatccgt cacagaccac
     7081 gacatctgct ttccctgtga ccaaatcatt tccctcaatg ttaccgatga aattcaagcc
     7141 actttttttg aatagcatgc tggcttcttt ggattgaaca tttccttttc cttcttcgct
     7201 ccccacactc agtaacccta gtttagggtc tttaactcca ataagtaatt tgctaaacac
     7261 agatcctagc accccgaatc ccagtaactg agatggtttg caatcaaggt tggaacccag
     7321 atcaagaata gtttggtttg gggcagatcc tactataggg ccgcctatgg caggcctttc
     7381 gatacccttt attgttccca gcaaaacaac agcggcggcc attgttcccc ctgtggaccc
     7441 catagatatg acggcatcgg cggttttatt cttcaccaaa ttagtagcta ctaagatcga
     7501 cgcggtgggt ttttgccgta aggctaaggc cggcggttca tgatcttcta tctttccatt
     7561 tgaaggtaca aaatgtactg gtaggccaac agtttcgtgt tttgctagtt cattttgaac
     7621 cagatcacta tcaccaacaa taaacacttc tgctaaacca cttttggcgg cttggaccgc
     7681 ccctgctacc gggagggatg gagcgttgtc tccgcccatc gcatctattg cgaccttcaa
     7741 taatcgatct tgtgtttctg gcaaatttag tctcctagtc accagccgat gctggtttat
     7801 ctactttcac tgaagcttca cctgatatga tgacctcccc atcttgtctg gttattatta
     7861 ctgtgcacgt tatgtgacta ctgttggttg tgtcttctaa ttttttaatc tgaccggaag
     7921 tttggatttc atcacccgga tacacgggtg atctgaatct cattttcatt ttgcctgtgt
     7981 tataccaggc tgtgccgaag ctttcagcca ttatttccga aatcgtagct gcagccatca
     8041 taccgtgacc aatattacga ccaaattggg aatttttgcc aaaagcttca ttgatatgaa
     8101 taggattata gtcaccagat gcatccgcat acatacgaat ctggtccatt gttatcattt
     8161 ttttgatagg tattaggtct gcccctagtt tcaagttttc catcctatgt tgttttatgc
     8221 aggaagcata attgtacttt tgcctttcat cactgaaagt ccggaagaat ttaaaacaat
     8281 taagcttact accatgaatc tccattcccc acgcacgtta ttggaagcaa gggaagcaac
     8341 acatcttagt gtctcaccaa cttttacgga attcaggaac tccatttctt gaccaacatg
     8401 tagagttcca ccaggtatct gtaagtcaac aacgactccc ctgaggctta aagctgcaac
     8461 cgccataggc ggtgatagtt ctatacctga tttatcaaat tcacggctcg atttatcttg
     8521 taccgcttcc agatatagag caacttcttt ttgtcctaga gtatatgttt ggttcgatat
     8581 ctcatttccg ctttctagta gagaataatc taatggcaac atctgctcac aagtacttaa
     8641 tttaatccag aattcaggtt aagaaaatcg tggtgtacca ctatataatg aatcttccaa
     8701 agcaattata tagccaccta ctcaaggtaa gtttacctgc cttataaggt agggtccttc
     8761 gagaagatag cataaattat caaaacatgg atgaattcga tcgaatcaaa tagaatttta
     8821 ggcagttccg gaaggagttt tattatttga ttgatataga gctgattaga gaaaaacctg
     8881 aatttgttat ccagtctttc aaaaggcgag gaaaagagct tccactcact gagataagtg
     8941 acttagatca aaggcgcaga gctgctataa ctgagattga ttccttaaga tctaggcgta
     9001 atgctgtctc gaaagattta gggcagtcta aagataaacc tgccgaatta atagaagaaa
     9061 tgaggaatgt gggatctcag atcaaagatt tagaggaaga ggaaaggacg ataagcggga
     9121 gactgaacca agtaatgctt gatgtgccca atcttcctag cgaatcctcg ccagatggcc
     9181 ctgatgagaa tagtaacatt gttattcgaa ccgtcggcag tattcctaaa tttgatttta
     9241 atcctaagcc acactgggag ataggtgaga atctggggat aattgatttt caaaggggag
     9301 ttaaattagc caaaacccga ttctatttac ttaaaggtca gggggcccgc ctccagaggg
     9361 ctcttatctc ttggatgttg gacttacaca ctgagaagca tggatatagt gagctgtact
     9421 tgccttatat ggtaaatagg gacacggtca cagcttctag ccatttgcct cacttcgagg
     9481 aaaacatgta ccacgacgcc gaagatgatc tgttcatggt accaactgct gaagctcctg
     9541 taactggtat gtttagagat gaaattttgg atgccgaaac cttacctttg aaatttgtct
     9601 cgcacacgcc ttgttggcga agagagaaat tttctgctgg tagagataca cgcggaataa
     9661 agagagtgca tcaatttgac aaggttgaaa tgtataaatt tgtggaacca gataaatcct
     9721 atgaggagct tgaattattg ttaaatgatg ctgaagcagt atgcgttgaa ttaggtattt
     9781 cgcataggat tctagagtta tgcacagggg atctcggctt cgcggcagct aaaagctacg
     9841 atgtagagat gtggagtcca ggtactcagg aatggctgga ggtgtcctcc tgttctaatt
     9901 gtaccgactt ccagtctagg agagcggcta ttcgttacaa agcctcagaa aattctcgtc
     9961 caagattgct gcacactttg aatggctcag ggctcgctgt acctcgggtc atgattgcta
    10021 ttcttgaaaa ttatcaactg cctgatggaa cggtaaaaat acccgacgtt cttaaaccct
    10081 acgctggatt cgacctcata actggtgact gagagggata ttgttagtaa aaattgaatt
    10141 taaggagaaa ttagtatgta cgtaacacga caggaagacg ttgatcctca agaagcagga
    10201 gacgctcctc ttttttatgg tgggagagtg acgagacagc cccttgtcgg tcgtacggaa
    10261 cctgaggatt ataactttgg gcttgtttct ttctatgcag gcgcaaaaaa ttattttcac
    10321 acacacacta gtgatcaaat attgtttgcg acgaaggggc aggggatagt agctaatgaa
    10381 tcagaagagg ttcaaatggg agccggagac actgctttta taccggctgg tgaaaagcat
    10441 tggcatggtg ccgcggatgg acatgacttc tctcatattt cattaacacg tccagggagt
    10501 gtgactgaga tgtttaagcc tgaatcgtag tattaaaagc tactgaatac atattggctg
    10561 ccaacactgt tctgtattat tgaattcggt agttccttca caagggataa aattgctttc
    10621 tgacctgtgc agtgggcagg caccaaccag tcaggatcta atgactttaa atcggagata
    10681 gtattttgta tagtggctgt atctagtcca tctggtagat gaaaacctcc gatgattgca
    10741 tggattttgt cttctgctgt taattttttc gcataattgc aaatattaac tattccagaa
    10801 tggccacacc ccgtcattat tattagtccc ttacccctta ttttagctat cagagcttga
    10861 tcatctaacg taagaggatc tggataccat gagccctcag taaatacttc ttgtcccgcc
    10921 aaaccttttt catagctggt gactcgatct acttctcctg ttatgagtaa tgaattgttt
    10981 aataaaaagg atggaagacg tgattcttct atcacttcga ggccaccttc ttgtagagca
    11041 aatttgctgg cggttggtaa aatacgaggg gttttaccgg gaagccggat ccttctttta
    11101 ttccaaaatt cgggatggat tattatcggt gtgttttgtg aacctaaatc atttaaaatc
    11161 ccatcaattc ccattgtgtg atcaaaatga ccatgactaa agacgatggc ctctgcatcc
    11221 ttaggcgata tgcccaatat tctcatattt tctgagacgc ccattggcga tactccggca
    11281 tcaaatagta cggaatgctg attactgtta ttttgaattg tcacccaggc cgaaaaacca
    11341 tgctcagctc taagctgtga tttaacaaaa ccgtctaccg ttaaaggtgc agcaacttct
    11401 acctcggttg aaggtctttt gacgggccct gtatcgctta ggaggctgct aatgtgatta
    11461 tccatcaata cagtgacaga taagtgatct atgactgcag gagatatttt aagcatgggc
    11521 cggctaaccc tccatgatcc aattatttta tgaatttacg gcgtagttta atacgtgagg
    11581 taaagtagat atttttcctt gttgcgtaaa tgtgcttgat atcactagaa tgtgctctat
    11641 tctaagccaa aaaaaattat gatcagaaat atttggaaaa ctggagaagg ttaatgtttg
    11701 ctgcaagaat aacaagtcct aagaaaattg aaatagagga tatagagact ccaaccataa
    11761 aagagggaca atgtttaata aaattggaaa ggtggtcggt ttgtggaagt gacattagac
    11821 acgcttatgg tccagtgatg ccagaagaag aatatcctat gaggcatgga ggtgcgtgcc
    11881 atgaatgtgc aggcactata gttgaaagca agtccgataa atttaaagtg ggtcagaggg
    11941 taatagtctt acctagccaa gacgggccag gcggccttgt tgaatactac ccgggcgatg
    12001 aaagcaggat ggcacttgta cctgatcatg gtgatttagg tgaatggatt ctttgccaac
    12061 cgtcagggac ggttttatat tcgtgccagc aaatgggaac tatattgggt aaagatgttg
    12121 ttgttatggg tcaaggtagt attggtctat ccttcacggc tatcgttgcg agagctggtg
    12181 caaggagagt tattgctgta gacccactgg attaccgatt agagtggggt aaaaagtttg
    12241 gtgccactca caccataaac cctgataggg acaatgtgga tgaagcgctt gctgaaatca
    12301 cagatggcaa acttgctgat atatctgttg aagcctctgg ttatcctgac ggcttgaatg
    12361 cagccattag aggtgcgact aaacatggaa aggtgataat ttttgggatt caggaaggcc
    12421 cacgaggaaa aaccactgaa atagatactc aatacttatt acggaatgcc ccaactatta
    12481 ttccgacctc gggagcagga agtggcgatg ctataggaca tatagagagg atgattgaat
    12541 taaaggatcg aggttggtgg aatccagggg agatgaaaac gcatgagaca aatttctcag
    12601 acgtcaatga tgcttacgac cagtatgaac aataccaaga cgatattatt aaagttgtaa
    12661 tgaacaacga gtaagtttta tttggtaccg aaaaaggggg agactaatat gtctccccct
    12721 tttttttatc catatttatc cgaaaattag gtaaaacagg agtattacca tgaaaatcac
    12781 atcagtaaag gctgtacctg cttcacacac caccactaga gcaggtgttt ctgtttctag
    12841 gaattatgtt ttcgtaaaaa tagaaacaga tgaaggtttg acagggtggg gggaagccac
    12901 ttgcggacct ttaagcgtgg ctacgatggt tgatgaattt ggtgaagtta ttaatggtca
    12961 gaatcctatg gagatcgaga aacactggca gactctctat catcattttc atgtccgtgg
    13021 cggtattgtt cagatgtcgg caatcagtgg aattgaaatt gcattgtggg atataaaagg
    13081 ccaggcacta ggcgtacccg tttatgaact cttaggtgga aaaataagag atagaatttg
    13141 gacctatggc agatgggatg gcttaacccc tgaagcagca gtggagaatg cgatgttcca
    13201 cgtggaacag ggtcttacag ccttgaaggg cgatcccttt gagcatcggg gcatatttat
    13261 tgataagaaa tcagaggatt tagcagtatc aaaaatgcga tcagtgaggg aagctgttgg
    13321 ggatgaagta gaactttttg ttgaagtaca cggaaggttg gcgccctctg atgccatcag
    13381 ggtagccaat aggttggaag aatttagacc ttttttttat gaagaacccg tgccacctca
    13441 aaatttggaa gccctcaaga aggtcgctga taatgtgaat atcccgattg ccactgggga
    13501 aaggcttctg accaaatttg attttgcaga tttattgcct ctgcatgccg tagacctgat
    13561 acaaccagat gttgttcagg cagggggtat tcttgagctc aaaaaaattg cggccatggc
    13621 ggaggcttat tacgtaggct ttcaaccaca taatccgtac ggcccttttt gtacaattgc
    13681 tgcacttcat ttggatgcat gcactccaaa tttcttgatt caagagggtg gtattcatcc
    13741 ttggtttcag gatgcaacga ctggcaattt cccaatccaa aaaaatggct actttggtgt
    13801 gccaacaggc ataggtttag gtgtagcaat gaatgaagat tggttgaagg caaatccctg
    13861 gagcgataat gcgtatcctt ggagacctaa tttgggtgcc aatccgtcac gtcaagatgt
    13921 gttttggtct taaacgataa atctttcccc ccatatttgg ttgcgatttt tgacctcaga
    13981 tttggatata atgccgctgg aagctcattt gcaatttggg ttgtaacaga aaatgacaga
    14041 aagggagaaa atgactatgg tttcacagct acgcatttac actattaacc gaggcatgat
    14101 ggactcttgg ctcaacctgt tccataaaca tcttaagcct aaccatgaga agtatggtat
    14161 accgatcgag tctagttggg ttaacgctga tcggacagag ttcatatggg tgagaagctt
    14221 cgataatgtg gaggtgattc aagctaaaga ggatgagtat tattcatcac ccgagcgtat
    14281 tgcgttggga gaccaaccga aagtccatat tgccaaaacc gaggtccgag tcgtagagcc
    14341 cactaactcc taggttcaaa ttttccacga atatctaatt aagcctagaa ttttgaacaa
    14401 tacgagcagg agaagtggtt ttggcagtta cagcagtaga tataaaaagt cgtgtcgaat
    14461 ttgattcagg gacttcttgg ggtaaatttg gaccttatga aaggatagat ggagttgtgg
    14521 agtttggggt tgatccagat aatgctgcca attccggaat cattgatttg catcattctc
    14581 cggtggatag tgctgggtta gtaaatttca gctctgattt tgttttattg acaccatcga
    14641 ccagggaatc ttctcgtctt cttgtggatg ttgttaatag gggaaggaaa agagcgatat
    14701 ctgattttaa tatggcatct cctaacttga ctccctcgtc caccatcgag cctggagacg
    14761 ggtttctttt tcagcgtggt tacactgtgg tatcgattgg ctggcagtat gatgtgtatc
    14821 gcagtgggtc tctactcgga atggacccac cacttgttga acttgatgga aaacctgttg
    14881 aaggtaccaa cttagtggaa attcggccga atgaaagaat caaaaattcc ttacttgcta
    14941 accgggccca taaaccctat cccgctagtt cgaccaataa tgctaaagac acaatgtatg
    15001 ttaaagattg ggaggatggg ccgcaggaag aaatacctag aagcgaatgg tctttttcta
    15061 aagaacaaaa tggaactgtt gttcccgatg ccgagtatgt atatatggaa tctggatttt
    15121 gcccaggtag aatttatagc gttgtatatg aggcatcaaa ggcagtcgtt tcaggatcca
    15181 ccttaatgtc tattcgagat attggttcct ggatcaaata tggtggtgtg aactccccat
    15241 taaaaagtcg acctacatat gcttatgcct atggaatatc tcaaacggga agacttttga
    15301 gacattattt atattcaggt atgaatttag atgaaaaggg acgccaggta tatgatggac
    15361 ttctgcctca tgtggctggt ggtagacgcg gggatttcaa tcacagattt gcacaacctt
    15421 cacaacaatc ttctcctgga ttcggccaca tgtatccttt tgcagatgaa cctacagagg
    15481 atccgtatgg caggaaattg gaaggacttc aagatagaca gaaaactctt ggtggactgc
    15541 caaaggttat ttataccaat acctcggcgg aatattggag gggtgatgcc tcgttatctc
    15601 atattgatct gagaggtcgc caggatcttg atttccctca aaatactaga agttacctat
    15661 tctcaggaac tcaacatgta ccaagggaac tcccccaaat gaaagatcct ggtccggatg
    15721 gatcgctcgg actttatggg ttcaatgtag ttgacttccg accccttctt agatcagcac
    15781 tttgcaactt agtatcttgg gttgaagagg gtcttgaacc accagaaagt aaggtcccaa
    15841 ggcttgctga tggcactgct agtaccattc ctgatgttct agaagtattc acgggaacgc
    15901 ttggattaaa gatcccagat cattctcaaa tgtggcgtct tagggaaatg gatatgggtt
    15961 tacgagaaga tattggcata gccacatatc caataaagga aggcagagaa tatcctagat
    16021 ttgtttcatc agtggataac gacggaaatg aagtggctgg tattcgaatg cctgatatat
    16081 cggttccagt tggcacccat acgggctgga atcctcgcga cccatcaaca ggagcccctg
    16141 atcagattat ttctatggtc ggattcacaa attattttcc agccactggg aaatctttta
    16201 ggccccatga tgatttaaga aattcaaata atgacaggta ttcgtcaaaa gaaaattatt
    16261 tgaaaatggt ttctaaagcc gcagagaaat tggttgagga aagatatcta attaaggaag
    16321 acattgatgt ggtgctgcaa aaatgtgccc aaaggtatgg cgaggccatt tctagaggga
    16381 atcaagttta agtagctaat atgttggtat tacatcaaat aggtattaaa ttccgatgaa
    16441 cgaattttaa ctaagcgaag cacctgttgc atcggtgtat atgctgtaaa ttgagcgact
    16501 tgctgtaata aataaacgat tgcgtttgtg tcccccaaat gtaagattgg acggtacttc
    16561 cggtaaatat atcctaccaa tcaagacgga tgaatcgtta tatatagtta tagaattagt
    16621 tcctcgctga gcagatgccc acagatttcc gtcagtatca cagcgcatac cgtctgtgga
    16681 atcctcacct gtctctgtaa aaatttttcc ttccgctaag ctgtcgccat tgtttgatac
    16741 ttgataaaca taaatagcat tagggttatt tgagccagaa tccgatacat aaaggtccgt
    16801 gaagtctggt gagaaacaaa gcccgttggg acgaacaaga cctgttgctt ttactgctac
    16861 tgctccggat gaagggtcaa ttctatacac tgcggtgggt aattcaggtt cagatcggtc
    16921 tccttcataa ttatttaaaa ttccatatcc ggggtccgta aaccaaattg caccatcttt
    16981 atgaatcgca acatcattag gggcatttag ttttgaattt ttgaaggagt ccgcaattat
    17041 ggtgattgac ccgtcaaatt ctgttctagt gacccttcta gttaaatgct cgcaggtgat
    17101 gagcctaccc aagttatctc ttgcgttgcc gttagcatta ttggatggat tacgatattc
    17161 actaacgatt tcggtttctt ccgtccatct aaggattcta ttatttggta tatcgctaaa
    17221 cagaagatat cttccatcac caaaccacgc tggtccttcg gtccatctag agcctgtata
    17281 aaggcgctcc attccagcat ttccgagaac atagcgtttg aaactttcgt ctattacctc
    17341 cacaaatggt tctggatacg gggtggtatc tttttcccaa tttcttacgg tactatttat
    17401 attcatgaaa ttgaattcct tatgtatagg tcaatcatga gactgtgggt agtaaatttt
    17461 ttagtagaga ctaattccaa agggaataaa aaatatttag gacagcctta ttttatccaa
    17521 atagctgacc tcagcaagtt tattacacag aacagatagg ttgaagtata ttgaataagt
    17581 tgagaaacga gacaccgaaa ctttattatg gttggctggt tctcgccgta tgtttcttta
    17641 ttgtgatgtg ttcatctggt gttcggtcta gtatgggtgt ttttataacc ccaatggaga
    17701 cggatatgca gtgggcccga aacgatatta ccagggtact ttctttggga atatttgttg
    17761 gggctttgtc ctttatattg atgggctatc tttatgatag atatggtggg cggcgggtga
    17821 taacggctgc tctaataatg gtcgggatat ctgttttttt gctaagtcgg gttaactcca
    17881 ttttcggatt aattttgata tacgggatac ttggaagtgt tgcaactagt ggtgcttctt
    17941 ttgtaactat tcactcgtta ttggcaaaat ggttctacag acggagaggc ttagctatga
    18001 gcctgtctgc tgccggaggc tctatcggcc ctttgctgtt tgcaccattt tgtgcgttcc
    18061 tgatagaagc ttttgattgg aggactgctt ttttattcat aggtggaatg gttttgttta
    18121 taggagctcc tctggcggct cttttcctaa aagatgatcc actgggccag attccagata
    18181 gtgaaaggga tgaaaccggg gttcaagata aagaagaagt gcaggtgaaa atggaaggac
    18241 cattatttac cagtcgttgg aagcaggctt tatccacttc ccctttctgg cagttatctg
    18301 cggcttatct tgtttgtggt attaccacta atatcatttc cgttcacttt gttccatttg
    18361 ctgaagatca aggagttccc aaaataaccg ctgccacgat ttttgggata atgatggggc
    18421 taaattctgt gggggtaatt tctgcaggag tattatcgca aaaattcagt cagcatagag
    18481 tgttaggttt tacatatgca ttgcgtggaa ttgcttattt agccttgtta acaattggtg
    18541 gaaccactgg aatgtgggcg tttgccttaa tagctggaat gtcgtggata gccacggctt
    18601 cagtaacttc ttccctcgta gcagatatat acggggtaag aaatttaggg acattgaatg
    18661 gcatgtctaa tatggttcat cagataggag gcgccctatc ggtacttgtt gccggggaac
    18721 ttcaggcatg gactggatcc tatgagattc cttttgcgat tgctggagca acattactgt
    18781 ttgcaagcat aatggctttt agtgtcaacg agaagaaata ctcatacaga tactctagag
    18841 tgcctgttgc agccgctcct tcttaattta taaactttct ttccatgata tgtagtctga
    18901 ccgtcccctc tctttaaatc tgggattgct gaatctatcc ataaactctt tcaaggtttg
    18961 gatcgcttgt tcattggaat ttggatcgta gtgaatccac ttatctaaat gttcttccga
    19021 ccggaagaaa ttcatgtttg ctcaatttct gccggcgatg tctccgcctc ccaaaaatcc
    19081 gaatccgtaa atggaaacac ccacaattcc tttagcaggt attgaactaa gaatctcgcc
    19141 atccttaact gtcacactta ttggatcatt acaatctagg caaacactat caatttggat
    19201 tgttttacca gggaataacc agcaaactgc tagagattca aaagctcatt gtccccacca
    19261 tttgctttca ccatctacgg taattttgta atgagttggt atgttgttaa acggtgcaaa
    19321 actgccaata gtatctgtgc cggggactag ccagtagcct gccatcttcc ctggacccat
    19381 aagatcattt agagatgttc tcgcctgctc tacctctacc ccgagtgatg tggctatttc
    19441 tgtgtaatga ggagccaccc cagtcgatac gatggttttc attatatggg tgtacagttg
    19501 atcaagagtt tttaaatcgg tcattaataa ttgccttttg ctttcggtta tctgatccag
    19561 gtctatatct ttaaagagta caatagcaat agattggttt gttagcaaaa caaggtcaac
    19621 attaacgatt tatatgagta taatggccat gctattttaa gcagcgacct tagaccatca
    19681 ggagatatcc agatcaaatt actaggacag cgcacgattt tttatggttg gatcgtggtg
    19741 ggtgttatgg caattgctag tgcagttagt atgggcatgg gctcattaaa tttcgggtta
    19801 tatatcaaac caatggggga gcagttaggt attggacggg cttctttcgg ctggtcttca
    19861 acctttagga gcatttcaag tgccgcaacg agccctatta taggcagatt gctcgatagg
    19921 catggtgcca gatggattct tgcaggttca accataatta ctggattgtg catggttggc
    19981 ctcgcctata cacgagaagc atggcaactc atggttttgt tttcaataat ggggttggtc
    20041 ggtatgagtg ggcccggtgc tcttactaca tctgtgccgc catccaagtg gtttgttcga
    20101 aacagaggaa aagctttagc cgttacagca gtaggtgttt ccttaggggc cctaattttt
    20161 gttccgctca ccc
//
LOCUS       DASCNS010000025         3401 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000233314, whole genome shotgun sequence.
ACCESSION   DASCNS010000025 DASCNS010000000
VERSION     DASCNS010000025.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000233314"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(206..787)
                     /locus_tag="QF838_00940"
     CDS             complement(206..787)
                     /locus_tag="QF838_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59293.1"
                     /translation="MTKEQNNIDPEEQNEYASSSDAETNDQSTDEPVEPPQKNNDTLQ
                     VENIPEGSIIFRQSHLSWLIPSSIWIIGLVVVDLQIGLLGGLGILLAPLIAAPRYLRW
                     RQTVYHLSNDSLYLTMAGLPVIQKSKIYQVKFDTIARGENGKVQIGIRHGMFGKTLGY
                     GEVSIVFDDKRVARLSYISEYQSFIDYINERTG"
     gene            complement(854..928)
                     /locus_tag="QF838_00945"
     tRNA            complement(854..928)
                     /locus_tag="QF838_00945"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(894..896),aa:Asn,seq:gtt)
     gene            complement(1037..2071)
                     /locus_tag="QF838_00950"
     CDS             complement(1037..2071)
                     /locus_tag="QF838_00950"
                     /inference="COORDINATES: protein motif:HMM:NF028222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO59294.1"
                     /translation="MKAAFYDGNGTMVVRDYPDLTPGPGEVLIKVRSTGICGSDLNMN
                     LDKSGPDSDPAGHEVAGEIVSVGKGVDRKHISSRVAVEVLGSGRACQNCWYCRQGQYI
                     HCPNRGSGVSGGFAEYIVRKIEGCYPLSDKMNWEEGALVEPLAVSVHGVRIGNMVGGD
                     TVAVLGSGTIGLTAITAARALGAGKIFATARHPQQAAAALSLGADAVGTKGESEFTQM
                     VLDATDGRGADLTIETVGGSRGDTLRQAIEVTRRQGRIVILGVFYGEHSINWTGPVLK
                     EQTIQGSICYGIINGQHDFETAVEILSRPDCNLSQIVTHKYSLEDIDIGFKTAYDKSS
                     GSIKVQVQQT"
     gene            complement(2390..>3401)
                     /locus_tag="QF838_00955"
     CDS             complement(2390..>3401)
                     /locus_tag="QF838_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59295.1"
                     /translation="LVKKLLKKADVFLEDRKPGFLESLGLGYEVLKKTNKKLVMTSLT
                     AFGQDGPYRDYKSNDLVALAMGGIMHSCGYDDVPDSPPIRPSGGHGDFISAHYGLIGT
                     MSALFYRDFTGVGQYLDVSAHEACSSTTEASIPTYLFLNKEVFRQTGRHQGATPTPKT
                     LCKTLDGKYIIVFQLFNNLNSWLSLVKWMSEKGMAEDLEDEKYKDMARHITSNTKDSD
                     HAFEVVRNFILNHTADEIYRGAQGLKYPWGIVRSPEENLDDPHFLDDREMFEDIPHTE
                     FGNEVTFRYAGRPYRFKGTPWNAFRSPLVGEHTAYILKQDLEYSEQEIKNLRLTGIIS
                     TK"
BASE COUNT          905 a          753 c          655 g         1088 t
ORIGIN      
        1 aagtacgtag gtgaccaggc gtcatggaga tcttttctat caaaaatcca ttctccttac
       61 caaattcaat tcttgtccct aggaaattcc gattatttac ttttgaaaaa tctagaagag
      121 attgaacaat tcggaataaa ggtgaaggga atagttgatg attaggtttt caatctggat
      181 caggccttcc cgctcactct ccttgttatc ccgttcgttc gttaatgtaa tctatgaaag
      241 attgatattc tgagatatat gaaagtctcg ctacacgttt gtcatcgaac actatactga
      301 cttccccgta tcctagggtt ttcccaaaca tgccatgcct aatacctatt tgcacctttc
      361 cgttttcacc tcttgctata gtgtcaaatt tgacttggta tattttgctt ttttggatta
      421 caggaagtcc ggccatggtg aggtacagag agtcattgga taaatgataa acagtttgtc
      481 tccatctcaa atatcttgga gctgcaatta gtggggctaa aaggataccc aaccccccca
      541 acagtccaat ttgcaaatcg acgaccacca atcctattat ccagatagag gatggaatta
      601 accaagaaag atgggattgt ctaaatatga ttgatccttc aggaatattt tcgacttgca
      661 aggtatcgtt gtttttctgg gggggttcca cgggttcgtc agttgattga tcgtttgttt
      721 ctgcatcgga acttgatgca tattcgtttt gttcctcggg atcgatatta ttttgttctt
      781 tagtcactta ttcaccagct ctagtacttg agataattag tcgttttaga attcaataaa
      841 tacctaaggt aattggctcc gcaggctgga ttcgaaccag cgaccgatcg gttaacagcc
      901 gattgctcta ccactgagct actgcggaac gtttttagtt cgcttcaact taagttccat
      961 gtgcctgcaa actagtttcc taataaaatc agcatctgta attgtctttt aaggagactt
     1021 tctagtcaag aaaagatcag gtttgttgta cttgaacttt tatggagcca gacgacttgt
     1081 cataagcagt cttgaatcca atgtctatat cttcaagaga atatttatgc gtcactattt
     1141 gactaaggtt gcagtcaggt ctggatagaa tttctacagc agtttcgaaa tcgtgctggc
     1201 cgtttattat tccgtaacat atggatcctt gaatggtttg ctccttaagg accggtcctg
     1261 tccagttgat ggaatgttca ccataaaaca cccctaaaat tactatacga ccttgccttc
     1321 tcgtgacctc aatagcttgt cttaaggtat ctcccctact gccccccact gtctcgatag
     1381 taagatctgc tcccctgccg tcggttgcat caagtaccat ttgagtgaat tcactttctc
     1441 ccttagttcc tacagcatcc gcacctaaag ataaagctgc agctgcctgt tgcgggtgtc
     1501 ttgccgtagc aaaaattttt ccggcgccaa gagccctggc cgctgttatg gctgttagtc
     1561 cgatggtacc tgaaccgagt acggcaacgg tatctccacc caccatgttt cctattcgga
     1621 caccatgaac agaaactgcc aaaggctcca ctaatgcgcc ttcctcccaa ttcattttgt
     1681 ccgaaagtgg ataacaacct tctattttac gaacgatata ttctgcaaat ccaccactta
     1741 cccctgaacc tctattaggg caatgaatat attgtccttg tctgcagtac caacaatttt
     1801 gacaagctct tccgctaccc aacacttcca cggccaccct tgagctaata tgttttcggt
     1861 cgacgccttt tcctacggat actatttcgc ctgctacttc gtgacctgct gggtctgagt
     1921 cgggtcccga tttatctaaa ttcatattta aatcggagcc acaaatccct gttgatctga
     1981 cttttataag tacttctcct gggccgggag ttaaatcagg ataatctcta acaaccatgg
     2041 ttccattgcc atcataaaaa gccgctttca ctttaaaact cctttcactt caaatcgtat
     2101 ttatgcgaaa attttctaac ctaggacgaa agaggctcgg accaggaaga aaaacaacag
     2161 aaaaattatt attactggta ttaggcagcc gaggcaccca gctctccatg tgaaatggaa
     2221 tcctttagaa gcctttttcc ctgagagatt agtggttacc cgtttgtgag aattttcagg
     2281 cagattcatc acttatataa agtcctatga tctggagacg ttggaaattt ttgacatctt
     2341 cgtaaagatc tcatttcttt accgtatcta ataatggcat ttttcaaaat tatttggtag
     2401 aaatgattcc ggttaatcgc aagtttttta tttcttgttc agagtattct aagtcttgtt
     2461 tcaaaatata ggccgtatgt tcgccaacca gtggtgatct gaaagcattc caaggagtgc
     2521 ctttaaatct gtaaggtcgg ccagcatacc taaaggttac ttcatttcca aattcagtat
     2581 gggggatgtc ttcaaacatt tccctgtcgt ccaggaaatg aggatcatct agattttctt
     2641 ctggagatct gacaattccc catgggtatt tcagtccttg agcccctcga tatatctcgt
     2701 ctgctgtgtg attcaatata aaatttctga ctacttcaaa tgcatgatca gagtcctttg
     2761 tatttgaagt aatatgccga gccatgtctt tgtatttttc atcttccaaa tcttctgcca
     2821 tacccttctc agacatccat ttcactagtg aaagccaaga attgaggtta ttaaaaagtt
     2881 ggaaaacgat tatgtattta ccgtccaggg ttttacaaag ggtttttggg gttggggtag
     2941 ctccttgatg tcttcctgtc tgacggaaaa cttctttgtt gaggaataaa tatgtgggaa
     3001 tcgacgcttc tgtagttgag gaacaagcct cgtgggctga aacatctaaa tattgtccaa
     3061 cacctgtgaa atctctgtaa aaaagtgctg acattgtgcc aattagacca tagtgggcgg
     3121 atataaagtc tccatgtcca ccactaggcc tgataggagg gctgtctggc acatcatcgt
     3181 agccacacga atgcattatc cctcccatag ctaaggccac caaatcgtta gatttataat
     3241 ctctataagg gccgtcttgg ccaaaggcag tcaacgaggt catcactaat tttttgttgg
     3301 ttttttttaa tacttcataa cctaaaccta gtgattctag aaaaccgggt tttctgtctt
     3361 ccagaaacac atccgccttt ttaagtagtt tttttactaa g
//
LOCUS       DASCNS010000026         3564 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000241169, whole genome shotgun sequence.
ACCESSION   DASCNS010000026 DASCNS010000000
VERSION     DASCNS010000026.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000241169"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /gene="leuB"
                     /locus_tag="QF838_00960"
     CDS             <1..1098
                     /gene="leuB"
                     /locus_tag="QF838_00960"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HJO59296.1"
                     /translation="PMKISIAVLPGDGIGPDVIAESIKVLESIGSRFGHDFQIKSGRV
                     GGNAIDDYGTPLPQETIDTCSEADAILFGAVGGPKWDDPNFDVRPEDGILAIRKSLGL
                     FANLRPVKVYPSLINSSPLKPEILEGVDMMVLRELTGGLYFGKPKKRWSTSRGRRGID
                     TLKYTEAEIVRILKVGFELAMQRRKKLTSVDKQNVLESSRLWREIAVEVGKGYPEVEL
                     DHILVDNAAMQLIRNPSDFDVIVSENTFGDILSDEASVLSGSMGMLPSASLSNLPGIS
                     GTSSKVGLYEPIHGTAPDIAGKGIANPIGSILSTAMMLRLSFGLHEEADAIEKSIDVA
                     LSEGYRTADISNDGENQISTMVMGDVISGAI"
     gene            complement(1113..2003)
                     /locus_tag="QF838_00965"
     CDS             complement(1113..2003)
                     /locus_tag="QF838_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HJO59297.1"
                     /translation="MDRVKRGHIVPGAEYEVVKDTPTTTVVDGHWGFGYVVTERLMKQ
                     TIEKAKSQNIAAATVYRQSHIGRLAAYPLIAAEAGMIAMITADSGRSAKAVVPFGGRE
                     KRLGTNPISIAMPSHLEGSFFLDMATSAVAAGKINLAQARGDSIPIGWLVKEDGSDTT
                     DPNELKNGGAILPLGGDQGHKGYGLSSIVEIFSGILTGLGFGHDPSGRHNDGCFISVF
                     NVSAFRDLNDFKEEVTEFASYLKSSETAPGFKEILYPGELEHRNEVRQKKEGIFVEES
                     TWENLKSLADSYKITSGLPF"
     gene            complement(2004..2162)
                     /locus_tag="QF838_00970"
     CDS             complement(2004..2162)
                     /locus_tag="QF838_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59298.1"
                     /translation="MPSIMPDDLEKIAVGLLIGAGVSEDEAKVVSRLSIAANLAGHDS
                     HGIIQIPS"
     gene            complement(2178..>3564)
                     /gene="glpK"
                     /locus_tag="QF838_00975"
     CDS             complement(2178..>3564)
                     /gene="glpK"
                     /locus_tag="QF838_00975"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HJO59299.1"
                     /translation="FYPNPGWVEHDPQEIIESVFFCIREVMKVAEVSLNSIISIGITN
                     QRETTLIWDRKTGTPIHKAIVWQCRRTSEICQKLIGTYEAETITKATGLSIDPYFSAT
                     KIKWILDSVPGAYTKAKDGDLAFGTIDTWIIWNMTRGKSHVTDVTNASRTMLFDLETL
                     GWSKQILDIFDIPIELLPEICDSDGQFGLCETDIFREFNLPISGVLGDQHASMFGQAC
                     FSVGSTKCTYGTGAFLLMNTGKDIKISDRGLLSTLAWKVKGEVEYALEGSVFSAGATV
                     QWLRDELELIESSAEVEVLAHSVQGNGGIYLVPAFTGLGAPHWDMNARGTIVGMTRGT
                     NKGHIARAALEAISYQCEEVLKIMQQDSDCEISELKVDGGATGNELLMQMQADISNLR
                     VNRSSVQETTVLGAAFVAGIGSGFWNSKSEISLIWDSQRVYTPLMTNEERSQKLTFWA
                     KAVERSKEWIS"
BASE COUNT         1018 a          821 c          695 g         1030 t
ORIGIN      
        1 cccatgaaaa tatctatagc agtattaccc ggagatggaa ttggacctga tgtaattgct
       61 gaatcgataa aagttttgga atcaataggc agtcggttcg gccacgattt tcaaattaaa
      121 tcagggcggg tagggggaaa tgctattgat gattacggaa caccactgcc tcaggaaaca
      181 atcgacactt gcagtgaggc agatgcaatt ttattcggcg ccgtgggtgg ccctaaatgg
      241 gatgatccaa attttgatgt acgcccagaa gatggcatat tggctattcg gaaatcctta
      301 ggtttatttg ctaatttgag accagttaaa gtttatcctt ccttgataaa ttcaagtccc
      361 ctgaaacccg aaattttgga aggggtggat atgatggtcc tgagagaatt aactggtgga
      421 ttatatttcg gaaaacctaa aaaacgatgg agcacgtcga ggggaagaag gggaatagat
      481 actcttaaat ataccgaagc cgagatcgta agaattttaa aagtagggtt tgagttggct
      541 atgcagcgcc gtaaaaaact aacctctgta gacaagcaaa atgttcttga atcatcaagg
      601 ctctggagag agatagcggt ggaggtggga aaggggtacc cagaggtcga gttggaccat
      661 attttggtcg ataacgcagc gatgcaatta attagaaacc cctcggactt tgacgttatt
      721 gtttcagaaa acacctttgg agacattttg agtgatgagg catcggtttt atctggttct
      781 atggggatgt taccgtcagc cagtctatct aatctcccag gcatttctgg tacaagtagc
      841 aaggtcggcc tgtatgaacc aatacacggt acggcacctg acatagccgg aaaaggaatc
      901 gccaatccga tcggctcaat actcagtact gcaatgatgc ttagactatc atttggattg
      961 catgaagaag cagatgcaat tgagaaatct attgatgtcg ccctttcaga aggctataga
     1021 accgccgaca tttctaatga tggagagaac caaataagca caatggttat gggagacgtt
     1081 atatcaggag ctatttagaa aatgccatgg gactaaaatg gcaaaccaga tgttattttg
     1141 taactatctg ccaaagactt aagattttcc cacgtagact cttcaacaaa aatgccttct
     1201 tttttttgtc tgacttcgtt tctatgctct agttcaccag gatacaaaat ttctttgaac
     1261 ccaggtgccg tttctgaact ttttaggtag gaagcaaatt cagtaacctc ttctttaaaa
     1321 tcattcaaat ccctaaaagc tgacacattg aaaactgata tgaaacaacc gtcattatgt
     1381 ctcccggaag gatcatgtcc gaatcccaac ccagttaata ttcctgaaaa aatttctaca
     1441 attgacgaca aaccataacc cttgtggccc tgatctcctc ctaatggcaa gatagcccct
     1501 ccattcttca actcgtttgg gtccgtagta tcgctcccat cttcctttac aagccagccg
     1561 attggaatag aatctcctct agcttgagct aaatttattt tcccagccgc caccgcgcta
     1621 gtggccatat ccaaaaaaaa ggatccttcc aaatgacttg gcatagcgat agaaatcgga
     1681 ttcgtaccta accgtttctc tcggccacca aagggaacta ccgcttttgc cgaacggcca
     1741 gagtcagctg tgatcatggc aatcatgccg gcttcggccg ctatcagagg gtaggctgca
     1801 agacggccaa tgtgactttg tcgatacaca gtagcagctg ctatattctg agattttgcc
     1861 ttctcaatag tttgcttcat taatcgttca gtgacaacat atccaaatcc ccaatgccca
     1921 tctactaccg tcgtagtagg tgtatctttc acaacctcat attctgcccc tggaactatg
     1981 tggcctctct taaccctgtc aatctaactg ggtatctgaa ttattccgtg tgaatcatgt
     2041 cctgccaagt tggcagcaat agacaaacgt gataccactt tggcttcatc ttctgaaaca
     2101 cctgctccga taagtagacc tacagcaatc ttttctaaat catcaggcat aattgaaggc
     2161 atatttctcc tcagtattta agaaatccat tcttttgacc tttccaccgc cttagcccag
     2221 aaagttaatt tctggcttct ttcctcatta gtcataagag gagtatatac tctctgtgaa
     2281 tcccatatta aagatatctc cgatttggag ttccagaatc ccgaccctat accagccaca
     2341 aatgctgctc caagcacagt agtctcttga acgcttgacc tattcaccct gaggttacta
     2401 atgtccgctt gcatctgcat taggagttca ttcccagtgg caccaccatc caccttaagt
     2461 tcagatattt cacaatcaga atcctgttgc attattttca aaacttcttc gcattggtag
     2521 ctaatagcct ccagggctgc tctggctata tggcctttat tcgtccctct cgtcatacca
     2581 acgatagtgc ctctagcatt catatcccaa tggggagctc ccaaaccagt aaatgcagga
     2641 actaaatata tacctccgtt accttggaca gaatgagcaa gcacctctac ttctgcagaa
     2701 gattcaatga gttccaattc atctctcaac cattgaacgg tcgctccagc tgaaaaaaca
     2761 ctaccctcca aggcatactc aacttcacct ttaaccttcc aagccaatgt agacagcaaa
     2821 cctctatcag aaattttgat gtctttgccg gtattcatca ataaaaaggc ccctgtacca
     2881 taagtacatt tggttgaacc aacggaaaaa caagcctggc caaacataga ggcatgctgg
     2941 tcaccgagga cccctgaaat aggtaaattg aattccctga atatatcggt ctcacataat
     3001 ccgaattggc cgtctgaatc gcagatttca ggtaataatt cgataggtat atcaaaaata
     3061 tccaaaattt gttttgacca accaagggtt tctaaatcaa agagcattgt gcgcgacgca
     3121 tttgtcacat cggttacatg acttttaccc ctcgtcatat tccagattat ccatgtatca
     3181 atagtcccaa aagctaagtc tccgtctttt gccttggtat atgcccctgg cacagagtct
     3241 aatatccatt ttattttggt tgccgaaaaa tatggatcta ttgataatcc tgttgccttg
     3301 gttatcgttt cagcctcata agtaccaatc agtttctggc atatttcgct tgttcttcta
     3361 cattgccata caatcgcttt gtgtattggt gtgcctgttt ttctatccca tatcaaagta
     3421 gtttctcttt ggtttgtaat accaatggaa attatgctat tcaaggatac ttcagccacc
     3481 ttcataacct cacgtatgca gaaaaacaca ctttctatta tttcttgggg gtcatgttct
     3541 acccatcccg gatttgggta aaat
//
LOCUS       DASCNS010000027         1299 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000251724, whole genome shotgun sequence.
ACCESSION   DASCNS010000027 DASCNS010000000
VERSION     DASCNS010000027.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000251724"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="QF838_00980"
     CDS             <1..544
                     /locus_tag="QF838_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59300.1"
                     /translation="LIGATLGHFLRYKYGLDFDLILITLGVATVTGFALGMKSCAEVF
                     VGPFGGYFSDKYGRYKTLVLSSAFTSIGLIGLGNLDSLLGIFSILILVFVMGVMLMMQ
                     LLTLVGIVANDESRSQVFSVYNTFQDFGSALGPLVGLSLITANLLPMVYSISGVCLFL
                     LISGTCLFLNKRRPTSLETT"
     gene            571..>1299
                     /locus_tag="QF838_00985"
     CDS             571..>1299
                     /locus_tag="QF838_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59301.1"
                     /translation="MNSETDSQLALKKIWTFSGMDDSLPQMVELTGSEPVLPSVYKTG
                     VVAQSTIAASALAASEMWRFRTGKIQNVSVDMKHAAIGFRSERYTEVYGGDTSKIFAS
                     SGESGSLSDVHGFYKCGDGGWLQIHANYPAHKQGVLEAFNCESTKKAVQSTLLKMTSL
                     EAETYLTGRALPAAMMRTLDEWSLHPQGMAVSRLPLMEIEKIGEADPLDLSDNPKRPL
                     EGIKVLELTKVLAGPLMGRTLGEHG"
BASE COUNT          345 a          241 c          315 g          398 t
ORIGIN      
        1 ccttatagga gccactttag ggcattttct gagatacaaa tatgggttag atttcgatct
       61 tattttaata actttaggag tggcgactgt caccggcttt gcattaggta tgaaaagctg
      121 tgcagaagta tttgttggac ccttcggggg ttatttttct gataaatacg ggaggtataa
      181 gactctggtt ttatcttcag cattcacttc aattggactt atagggttag gaaaccttga
      241 ttcactgtta ggtattttta gcattcttat attggtgttt gtcatgggtg tcatgttaat
      301 gatgcagctt ttaactttgg ttgggattgt cgccaatgat gaatcacggt cacaggtttt
      361 ttctgtttac aatactttcc aggatttcgg ctctgctctc ggtcccctag tcgggctttc
      421 attaataaca gcaaatttgt tgcctatggt gtattccatt agtggcgttt gcttattcct
      481 tttgatatca gggacatgtc tatttctaaa caaaaggaga ccgacatcac ttgagacaac
      541 ctaaaatcgg acagcagata aggtggttca atgaatagcg aaactgattc tcagttggct
      601 ttgaaaaaaa tatggacttt tagtggtatg gatgattcac tcccccaaat ggttgagttg
      661 actggttcgg aacctgtatt gccatctgtt tacaaaaccg gtgttgttgc ccagtcaact
      721 atcgccgcct cagctttagc tgcttctgaa atgtggagat ttaggactgg caaaatccaa
      781 aatgtgtctg tcgatatgaa gcatgctgct atcggctttc gcagcgaaag atacactgaa
      841 gtatatgggg gtgatacatc aaaaatcttt gctagtagtg gagaaagcgg tagcctttct
      901 gatgttcatg gattttataa atgtggagac ggtggatggc tgcaaataca cgctaattat
      961 ccagcccata aacagggggt actagaggcc tttaattgtg agtcgacaaa aaaagctgtg
     1021 caaagcacac ttttaaaaat gacgtcctta gaagctgaga cttatctgac tggaagagct
     1081 ttaccggccg cgatgatgcg tactcttgat gaatggtctc ttcacccgca aggcatggca
     1141 gtatctaggc ttcctctcat ggagatagaa aaaatagggg aggcggaccc tcttgatctc
     1201 tcagataatc cgaaaagacc ccttgaagga atcaaagttt tggagcttac caaggtattg
     1261 gcagggcccc tgatgggaag gactcttggg gagcacggg
//
LOCUS       DASCNS010000028         2105 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000261567, whole genome shotgun sequence.
ACCESSION   DASCNS010000028 DASCNS010000000
VERSION     DASCNS010000028.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000261567"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="QF838_00990"
     CDS             complement(<1..603)
                     /locus_tag="QF838_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59302.1"
                     /translation="MSSEYFADDLPLNGIKVVETAQGVSGPYAAKLLASLGAEVIKVE
                     LPEGDWSRKAPPFLSETNHTESSALYLYNNTGKKSVVLDWRNSEGLHNLENIIKSADV
                     FIEDWDIAARKKWKLKPGFFIEKNPQLIELSITPFGLSGPYSSYKSTPLIQLALGGIL
                     NLIGNPEQEPLMLPGHQPDYLTGINGSNSVQIALWDRDLLG"
     gene            complement(612..>2105)
                     /locus_tag="QF838_00995"
     CDS             complement(612..>2105)
                     /locus_tag="QF838_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013225.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HJO59303.1"
                     /translation="ERIERQHSGGRYTVRERVEKMLDKGSFVEYGPMIGAAEYDDDGN
                     LIEFSPGAYVMGLGEINGRPVAIGGDDFTISGGSPHNVRKHPIDFMQPLASQYGIPYI
                     QLVEGVGHSAKSDEAKGHMGLPDGALWWRGIELLKKVPVAAGIMGSVAGAPAAFALMS
                     HFTVMVKGQSQIFPSGPPVVKRAIGEDLDKEELGGYKMHVHESGQVDNVADSEEEAFE
                     QIKQFISYLPNTTNDVAPRVETGDPPDRRPEELLNIIPPNRKRSYDPRKLIKLVVDNG
                     EFFEMRRHWAGALITGFARLDGYSVGIIGSDPMFMAGAMDGWAAEKYAHFVDLCDAFN
                     LPVVIFLDMPGFMLGSHSERKATMRRGVRALIASAEAEVPKIEFNVRKAYGVAADAAH
                     SLGHPNGLNLRFGWPAGEWGGIPIEGGVAAAYRREIENAPDPEAHRSMIEDRLLKLRS
                     PFRAAHKGDVVDLIDPRETRRLACRFVKLAQPMLEKLAQKPKRVVRP"
BASE COUNT          523 a          549 c          414 g          619 t
ORIGIN      
        1 ccccaaaaga tccctgtccc atagcgcaat ttgtacagag ttactcccgt taatcccggt
       61 cagataatcc ggttggtgtc ctggcaacat taatggttct tgttcagggt ttccaatcag
      121 gtttagtatg cctccaagag ctagttgtat caacggagtg gatttatagc tgctataagg
      181 cccggataaa ccaaatggag ttatagaaag ttcaattaat tgagggtttt tttctatgaa
      241 aaatccaggc tttagtttcc atttctttct agccgctata tcccagtctt cgatgaatac
      301 atccgctgat ttaattatat tttccagatt gtgaagacct tctgagtttc tccagtcaag
      361 gacgacactt tttttccctg tgttgttgta caaatacagt gcactggatt cagtatggtt
      421 agtttcagac aggaagggtg gggcctttct actccaatct ccctctggta actccacttt
      481 gataacctca gcacctaagg aagccaacag ctttgcagca tacggacctg aaacaccctg
      541 tgctgtttcc acgaccttta ttccatttaa tggcagatcg tccgcaaaat attcagagga
      601 catattttga cctaaggtct gacaaccctc ttcggttttt gggctaattt ctccaacata
      661 ggttgggcta atttcacaaa tctacaagca agccgccggg tctctcttgg atctatcaaa
      721 tctactacat cacccttatg tgcagctctg aacggcgatc gaagtttcaa taatctatcc
      781 tctatcattg acctgtgggc ctcagggtcc ggtgcatttt ctatttctct tcgataagct
      841 gcggcgacac caccttctat cggtatgccg ccccattccc cggcaggcca tccaaatctc
      901 aaatttaaac cattggggtg gccaagacta tgagctgcat cggccgctac cccgtaggcc
      961 ttcctaacat taaattcaat cttaggaacc tctgcttctg cacttgctat taaggctctc
     1021 actcctcttc tcatggtcgc tttcctttct gagtgggaac caagcataaa acctggcatg
     1081 tccaaaaata tgacaaccgg gaggttgaac gcatcgcaaa gatccacaaa atgtgcatat
     1141 ttttctgcag cccaaccgtc cattgcaccg gccatgaaca taggatcgct gccgattatc
     1201 ccaacactat aaccatccaa tcgagcgaaa cccgtgatga gtgccccagc ccaatgcctc
     1261 ctcatttcaa aaaattcacc attgtcgaca actaatttaa tcaatttcct aggatcataa
     1321 gatctctttc tatttggagg aatgatgttt aataattcct caggtcttcg gtctggagga
     1381 tcgcctgttt caactcttgg agctacgtca ttggtggtat ttgggagata agaaatgaat
     1441 tgtttgattt gttcaaaggc ttcttcttcg gaatctgcga cattatcgac ctgcccactt
     1501 tcatgtacat gcattttgta gccacccaac tcttctttgt cgagatcctc tccgatggcc
     1561 cttttaacaa ctggtggacc ggaagggaaa atttgagatt gtcctttcac catgactgtg
     1621 aaatgagaca tcaaagcaaa ggcagctggt gctcctgcta cagaccccat tattcccgcg
     1681 gcaaccggta ctttttttaa gagttcgatt cctctccacc agagggcacc atccggcagc
     1741 cccatatgac ctttggcttc gtctgacttt gcactgtgcc cgactccctc tacaagttga
     1801 atgtagggga taccgtactg tgaggcgagt ggttgcatga aatcaattgg atgctttcta
     1861 acgttatggg gactaccgcc cgagatagtg aaatcatctc cgccaatcgc aactggccta
     1921 ccgtttattt caccgagtcc cataacatag gcaccgggag aaaattcaat taagttgcca
     1981 tcatcatcgt attctgcggc tccaatcatc ggcccatatt ctacgaaact ccctttgtct
     2041 aacatttttt cgacccgctc ccggacagtg tatctaccac cactatgctg tcgctctatc
     2101 ctctc
//
LOCUS       DASCNS010000029         8831 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000291008, whole genome shotgun sequence.
ACCESSION   DASCNS010000029 DASCNS010000000
VERSION     DASCNS010000029.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000291008"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2325
                     /locus_tag="QF838_01000"
     CDS             <1..2325
                     /locus_tag="QF838_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HJO59304.1"
                     /translation="KLRISGLEGDIYRNLFAIGEKNREEILARYPKIQRRVSGYNLDE
                     FVGGSNFNMARFVVGSEGTLLTITEAKLKVVPIPKIKGLAVLHCNDLNESMEATVAAI
                     EMEPAAVELIGSMIIKQAQSNLAYSRLTSFIDGSPEALLVIELIADTDLELQSKFDEL
                     EARMQKGGWGYKLLRMTNSADQQKVWDVRKAGLGLMMNVPGDAKPLPFVEDTAVSPDV
                     LPEFVKRFDQIIKKNGTEAGYYGHASVGCLHIRPLVNLKNQEGIDRMVAISEEISDLV
                     LEFGGSLSGEHGDGLVRSGFNRKMFGDQLYEAFREVKSTFDPQGIMNPGKIVDSPSMT
                     DNLRYGTNYHTLSPDTGFAYRTEGGSFASAIEMCNGQGACRKVIGGTMCPSYMATRDE
                     EHSTRGRANALRATISGALPPQAVTQERMYEVMNLCLECKGCKAECPSNVDMAKLKYE
                     FLNIYHKENGYHLKNRFFGNVASLSKLGAFLAPLSNWMMSIPGSKQLLEQSVGIDSRR
                     PLPKFASQTFAQWFKARGGSPQKDVVKGDVVLFSDTTTNFNHPELGIAAVEVIEKLGY
                     QVILPNLKCCGRPMLSNGMIDAAKDNIAFNVEQIYPYVKNGAKLVGIEPSCVLGFLGD
                     FGDLMEDEKKSSEIAQSTMLIEEFFLYALENNPDIVFENPPENQNAVLHGHCHQKALV
                     GIEAAMKVLEHIPGLHASEVKSGCCGMAGSFGYLKDHYEISMQIGEETLFPQLRTEQE
                     DTLVITEGVSCREQIEQGTGRKSKHLVQVIADYL"
     gene            2616..3515
                     /locus_tag="QF838_01005"
     CDS             2616..3515
                     /locus_tag="QF838_01005"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO59305.1"
                     /translation="MKVGFIGIGLMGQHMVKHIINAGHDLFVYDINKEAANEIVAMGA
                     IFAENPAQVARSTEVVFTSLPRPQDVEEVTLGEGGLLSGASQNTTHFDLSTTDPDTIR
                     NVASLYEQKGATILDAPVSGGTVGSEKGTLCIMVGGDQTKYEKYKPILDVMGSEIMYC
                     GEMGSGAVCKIANNLIGMTLGVVLSEALSMGVKAGVDPMILYEAISASTGNTAHMKSY
                     PDTLFKGNFEPGFKLDLGAKDVGLATDLGRKLKVPMKVSNLIQQTYINAQNKGLGQES
                     TLAIAKLQEEISGVEIRNKDKAN"
     gene            3571..4809
                     /locus_tag="QF838_01010"
     CDS             3571..4809
                     /locus_tag="QF838_01010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HJO59306.1"
                     /translation="MRLVTQNMPHSESVSIFISIKAGSRDETRDEFGVAHFSEHMLFK
                     GTKDRPSPLLVTSPIESTGGMLNASTGYESTTYWCKIPKNDFSIGIEILLDMIQNSLL
                     KEDDIDSERGVIIEEIKSIEDYPDSKAALNLDKLMWGNNALGRDIAGSMQSVANITQE
                     TISSFIKNFYLPTSTVVSIAGNIDHDTLIQSVDQSLLIHPLTGYQPKFSSIKVVQNSV
                     RLDIEERDIEQIHIHIGYPGVSRNHPDRYSLDILDVILGDGMSSRLFQEIRERLGLVY
                     DIESGITHLQDVGDFRITLAVDKNKYIDAIKAVLIEVENLKTKLEDSEVVRAKRILKG
                     RMGLRMDDSQAVAAWNAQQELFNKRIVSESVIRKHIDEHDISTLQKAASQYLDISKLN
                     IAIVGDIESKEPILNSIAHL"
     gene            4954..6183
                     /gene="thrC"
                     /locus_tag="QF838_01015"
     CDS             4954..6183
                     /gene="thrC"
                     /locus_tag="QF838_01015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HJO59307.1"
                     /translation="MPHVKCLQCRECKSEYPIEPLNACEFCFGPLEVSYDYDSVAKAV
                     SRKSIESGPNTMWRYHDFLPVEKDSAVDISTGFTPLIHAKNLGRQLGLDHLYIKNDSV
                     NPTFSFKDRPVSVTTTKALEFEFEVLACVSTGNLMGSVAAHGAKAGMQTMVFYPADLE
                     QGKIQGAAVYGPTMVAVEGTYDQANRFCSELADAHRWAFVNINMRPYYAEGSKTLGYE
                     IAEQLGWKAPDHCVVPGASGELHTKIWKGLEEFEGAGLIDKAKTKMHLAQAEGCSPIV
                     EAFESGNNQVKPVRPQTIAKSLAIGNPAAGPYSLEVLRETGGTGVFAYENSIIDGIRL
                     LAETEGIFTETAGGVVISSLKRLVKQGKIKKDEVTVAFVTGNGLKTLEAMGDFVVPIE
                     TKPDLDTFQKKLSNQKD"
     gene            6198..6440
                     /locus_tag="QF838_01020"
     CDS             6198..6440
                     /locus_tag="QF838_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HJO59308.1"
                     /translation="MATKRVKFTFPQELIKEPVIYKLGVDFGIVTNIRRADIRDDMGW
                     VVLELEGESNVVDEGLAWVISTGVRVDPIAGDIIEG"
     gene            6482..6760
                     /locus_tag="QF838_01025"
     CDS             6482..6760
                     /locus_tag="QF838_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HJO59309.1"
                     /translation="MSVSVRIPTPLRKVTNGEDKANADGNTVSAIIASLDSQFPGIKS
                     RLCDDNGELRSFVNIYVNGEDIRFMEGVGSGVNSGDEISIVPAVAGGR"
     gene            complement(6771..7928)
                     /gene="tgt"
                     /locus_tag="QF838_01030"
     CDS             complement(6771..7928)
                     /gene="tgt"
                     /locus_tag="QF838_01030"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HJO59310.1"
                     /translation="MFNFTLEHSDGFARAGCIKTSHGEIRTPVFMPVATQGSVKALDR
                     IDLECLGTQIILGNAYHLYLRPGSKTIKELGGLHSFMNWNKPILTDSGGFQGFSLQHL
                     RKITDDGIIFKSHIDGSTHELTPESVVEIQTDIGSDVIMPLDVCLPTAASKNDLLDAM
                     NLTSTWLGRSIRSLDSKSDQSLFGIVQGGLHIDLRQKSADFLSSLNLPGYAIGGLSVG
                     ETKEKMYPTVEITSQLLPIDKPRYLMGVGSPEDLVEGVVRGIDMFDCVLPTRVARKGA
                     MYTKYGRRNVTRSEYKISDDSFDADCDCYACNHFSAGYIHHLFKAKEYLAYRLASIHN
                     LRFLMQTMADLREAIQQGLIKEYRETFWENYQPADQSARTNQSLHQVKRFE"
     gene            complement(7940..>8831)
                     /locus_tag="QF838_01035"
     CDS             complement(7940..>8831)
                     /locus_tag="QF838_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014035.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HJO59311.1"
                     /translation="DIHLIETILFTLGVATGIYGVLVGAGGGFILAPVLILFFSKDPE
                     IAAGTSLALVSINSISGFIAYRKSGFIDVRSGFLFAIAAVPGSVLAPFILDVMPGNIF
                     RTLFGVLLLSLAIHMFFRGSKAEDDSGYTSDDITLKPALPKWMSFTVKERRITAFDGT
                     IFNYRFNESMATIFNLILGFVSSFFGTGGGFLRTPILVSAFSFPVKVAVATSIFSLSF
                     YATIGAAAHAFNHNIEWYPTLVWGGLGLVLGGQIGARLAQRIKSFWILKLLLLVVLLL
                     GLQLLLEGLWPGLLNLPSAH"
BASE COUNT         2830 a         1729 c         1939 g         2333 t
ORIGIN      
        1 aagttgcgaa tatcaggact agaaggagat atttacagaa acctcttcgc tatcggagaa
       61 aaaaacagag aagaaatttt ggctcgatat ccgaaaatac aacgaagggt atccgggtat
      121 aacctggatg agtttgttgg aggaagcaac ttcaatatgg ccaggtttgt ggtcggatca
      181 gaaggtactc tcctcaccat tacggaagcg aaattaaagg tagtcccgat ccccaaaata
      241 aaggggttgg cggtcctgca ttgcaatgat ttgaatgaat ccatggaagc aacagtggca
      301 gcaatagaaa tggaaccggc tgccgttgaa ttaattggaa gcatgataat caaacaagct
      361 caatccaact tggcctattc caggcttacc agttttatag acggatcacc cgaagctctc
      421 ctagtaatag aattgatagc tgacacggac ttagaactac aatccaaatt tgacgaattg
      481 gaagcacgaa tgcaaaaagg tggttgggga tacaaacttt tgagaatgac caattcagca
      541 gatcaacaaa aagtttggga tgtgaggaag gcaggattag ggttaatgat gaatgttcct
      601 ggtgatgcta aacctttgcc atttgtcgaa gacactgcag tatctcctga tgtacttcca
      661 gagtttgtaa aaagatttga ccaaataata aaaaagaacg ggactgaagc cggttactac
      721 ggccatgcta gtgttggatg cctccacatt cgacctttgg ttaacttaaa gaaccaggaa
      781 ggcatcgaca ggatggtggc aatatctgaa gagataagtg acctggttct tgaatttggc
      841 ggttctttga gtggtgaaca tggtgatgga ttggttcgaa gtggatttaa tcgcaaaatg
      901 tttggtgacc agttatacga agcattccgg gaagttaaat caacctttga cccacaaggt
      961 attatgaatc ctggcaaaat agttgattcc ccctctatga ctgataattt aaggtacggt
     1021 actaactacc acaccttaag tcctgataca ggattcgcct acagaacaga aggtggaagt
     1081 tttgccagcg caatagaaat gtgcaatgga caaggggcct gtaggaaggt tataggtggc
     1141 actatgtgtc cttcatatat ggctactaga gatgaagaac actctacaag gggaagggcc
     1201 aacgcattaa gggcaaccat atcaggtgca ctgcctccac aagcggtcac tcaggaacga
     1261 atgtatgagg taatgaatct ctgcttagaa tgcaagggct gcaaagctga atgcccctcc
     1321 aacgttgata tggcaaaact taaatatgaa ttccttaaca tataccacaa agagaacggc
     1381 taccatctaa aaaataggtt ttttggaaat gttgcttctc taagtaaatt gggcgccttt
     1441 ttagcacctc tgtccaattg gatgatgtcc attccaggct ccaagcagct gctggagcaa
     1501 tctgtcggaa tcgattccag aaggcctctc cccaaattcg catcacagac attcgcccaa
     1561 tggtttaaag caagaggtgg ttcacctcag aaggatgttg ttaaaggcga tgttgtctta
     1621 ttctcagata ctacaactaa ttttaaccac ccagaattgg gaattgcagc agtcgaagta
     1681 attgaaaaac tgggctacca ggttatccta cccaacctga agtgttgtgg tcgccccatg
     1741 ttgtcaaatg gaatgataga tgcagctaaa gacaacatcg cctttaacgt agagcagata
     1801 tatccttacg tgaaaaacgg ggcaaaatta gtcggtattg agccgagctg tgtccttgga
     1861 tttctgggag attttgggga tcttatggag gatgagaaaa aatcctctga gatagctcaa
     1921 agtactatgt tgattgaaga attttttcta tacgctttgg aaaataatcc tgatattgtt
     1981 tttgaaaatc ccccggaaaa ccaaaatgct gttctccatg gccattgcca ccagaaagcc
     2041 ttggtaggaa ttgaggctgc aatgaaggtg ttggagcaca taccgggcct tcacgcatct
     2101 gaggttaaat ctggttgttg tggtatggca ggatcttttg ggtacctaaa agatcattat
     2161 gaaatctcca tgcaaatagg tgaagaaacg ctgtttccac aattaagaac agagcaagaa
     2221 gacactctag ttatcacgga aggtgtgtcc tgccgtgaac aaatagaaca aggaaccggc
     2281 cgaaaatcca aacaccttgt tcaagttata gccgattatc tataagcctc ttttaatccc
     2341 agtcttctaa acgattccca aactactacg catataagcc accaacaata tacttgattt
     2401 ggtcgccatg cttactagaa aaatccaacc gtttaaagga atcagagcca aaatgtccac
     2461 cgttataagt gatttactcc tccatgactc ctagcggttc tagatacatc cagttaagtg
     2521 atatccatga aacggattga ttcttttagg ctgtgctata aaatcaaatc tcattacgaa
     2581 ttatcagcca atccttttca aagggagagt accaaatgaa agtaggattc ataggcatag
     2641 gattaatggg tcagcacatg gtcaaacaca taataaatgc aggtcatgac ctgtttgtgt
     2701 atgacataaa taaggaagcg gcaaatgaaa ttgtagccat gggtgcgatt tttgctgaaa
     2761 atccagctca agtcgcccga agcacagaag tagtgtttac atctttacct agaccacaag
     2821 atgttgaaga agttaccttg ggtgagggag ggttactatc tggagcatcc caaaatacca
     2881 cccattttga tctaagcact actgatcctg acactattcg caatgtagcc tcactatacg
     2941 aacaaaaagg tgcaactata ttagatgctc ctgtaagcgg tggtacggtt gggtctgaaa
     3001 aagggacatt atgcatcatg gtcggagggg atcaaacaaa atacgaaaaa tacaaaccaa
     3061 tcttggacgt gatgggatca gagattatgt attgcggaga gatgggatct ggtgctgtct
     3121 gcaaaattgc caataatcta attggtatga ccctcggagt ggttttatca gaagctttaa
     3181 gtatgggggt gaaagcaggg gtagacccga tgatcctgta cgaagctata tcagccagta
     3241 cgggaaatac tgcccacatg aaatcttacc cggatacact tttcaaaggc aactttgaac
     3301 caggttttaa gctcgacctg ggagccaaag atgtggggct cgccacagat ctgggacgaa
     3361 aactaaaagt tcctatgaag gtatcaaacc ttatccagca gacttacatt aatgcacaga
     3421 ataagggctt aggacaggaa tcaacgttgg caatagctaa actacaggaa gaaatttctg
     3481 gggtggaaat tcgcaataaa gataaagcga attgatgaaa ggtttaatgt ttcattggta
     3541 aaacacaaga aatctatact agattctggg ttgcgattag taacccagaa tatgccccat
     3601 tctgagtctg tttcaatatt tatatcgatc aaagccgggt ctcgtgacga aacccgagat
     3661 gaatttgggg tggctcattt ctcagaacat atgttgttta aggggactaa ggatcgacct
     3721 tccccattgc tagttacctc ccctattgag tccacaggtg gcatgttaaa tgcgagcact
     3781 ggatatgaat cgacgaccta ttggtgcaag attcccaaaa acgatttctc aattggaata
     3841 gaaatcttac tcgacatgat tcaaaactca ttattgaaag aagatgatat tgattcagaa
     3901 cgtggagtga taattgaaga aataaaatcc attgaggatt atccggattc caaagcagcc
     3961 ttgaatttag ataaattgat gtgggggaat aatgccttag gtcgtgacat tgctggttct
     4021 atgcaaagtg ttgcaaatat cactcaagaa acaattagtt cgtttattaa aaatttttat
     4081 ctcccgacaa gcactgtagt cagcatcgct ggaaacatag atcatgacac tttaattcaa
     4141 tcggttgatc aatcattgct aattcaccca ttgactggtt accagccaaa gtttagttca
     4201 attaaggttg ttcaaaattc agtgagattg gatatagagg aaagagatat agaacaaatt
     4261 catattcata tcggatatcc gggggtctcc agaaaccatc ccgatagata ttcactagac
     4321 attttggatg tcattttagg agatggaatg agtagcagac tattccaaga aataagagaa
     4381 cgactcggtt tggtctatga catagagtcc ggaatcaccc atttacaaga tgtcggagat
     4441 ttcagaataa ctttggcggt cgataaaaat aaatacattg atgctattaa agctgtcctt
     4501 atagaggtag agaacttaaa aacaaaatta gaggattcag aggtcgttcg tgccaaacga
     4561 atcttaaaag ggagaatggg gttaagaatg gatgattcac aggctgtagc tgcatggaat
     4621 gcccaacagg agttattcaa taaacggata gtgagtgaaa gtgtcattag aaaacatata
     4681 gatgaacatg acatatcaac attgcaaaaa gccgcttccc aatatttgga tatttccaaa
     4741 ctaaatatag ctattgttgg agacatagag tcaaaagaac caatattgaa ctcaatagcc
     4801 catttataag gaaatgaact tgaaatcgtt tgttatttcc ttgacactga aatcactaca
     4861 ttgctaacat ttcctgcctg atcttgattt caattggtgg ctcaagttat acggttttag
     4921 acaactgtat tggaaaaact gaggagcaaa agattgccgc acgtaaaatg cctgcaatgc
     4981 agagaatgta aaagcgaata cccaatcgag ccgttaaatg cgtgtgagtt ttgttttggc
     5041 cctttggaag tttcatatga ctatgactct gtagctaaag ctgtctccag aaaaagtatt
     5101 gaatctgggc caaatactat gtggagatac catgatttct taccagtaga aaaggattct
     5161 gcagtcgata tatctactgg atttactcct ctaattcatg cgaaaaatct gggtcgccaa
     5221 ctcggtttgg atcaccttta cataaaaaat gacagtgtga atcctacttt ctcttttaag
     5281 gacaggcctg tgtctgtcac aacaacaaag gctcttgaat ttgagtttga agttctagca
     5341 tgtgtgtcta ctggtaactt aatggggtct gttgcagctc acggtgccaa ggcagggatg
     5401 caaacgatgg tattttatcc agctgatctt gaacaaggaa aaattcaagg tgcggctgtt
     5461 tatggcccta ctatggtagc agtagaaggt acatatgatc aggctaacag attctgcagc
     5521 gaactcgcag acgctcatag atgggcgttt gtaaatataa acatgcgccc atactatgcc
     5581 gaagggagta aaactctagg gtatgaaatc gctgaacaat taggctggaa agctcctgac
     5641 cactgtgtag tacctggagc atcaggagaa ctacacacaa aaatttggaa aggcctggaa
     5701 gaattcgaag gagctggctt aattgacaaa gccaaaacaa aaatgcacct tgcccaagca
     5761 gaaggatgtt cacctatcgt agaagcattt gaatctggta ataaccaagt caagcctgtt
     5821 cggcctcaaa ctattgcaaa atctttggct attggaaacc ctgccgctgg accctattca
     5881 cttgaagttc ttcgtgaaac tggaggcacc ggagtttttg cctacgaaaa ttcgataatt
     5941 gatggcatac gtttgttagc tgaaacagaa ggaatattta ccgaaacagc aggcggagtg
     6001 gtaatatcca gcctaaaaag gttggtcaaa caaggtaaga ttaaaaaaga cgaagtcact
     6061 gtggcctttg tcacaggaaa tggtctgaaa actttagagg caatgggaga ttttgtggtc
     6121 ccaattgaaa ccaaacctga tttagacacc ttccaaaaga agctgtctaa tcaaaaggat
     6181 tgaggtggta taagattatg gcaacgaaac gtgtcaaatt tactttccca caagaactta
     6241 ttaaagaacc ggtgatatat aaacttggtg ttgatttcgg tatagtaacc aacattagga
     6301 gagcagatat aagagacgac atggggtggg ttgtgttgga attagaagga gaaagtaatg
     6361 tggtagatga agggcttgca tgggtaattt ccacaggtgt gagggtcgac ccaatagctg
     6421 gcgacatcat tgaaggttaa ccagcctgaa ccggacgatt taaaaatcaa ggagaaaata
     6481 aatgagtgtg agcgtaagaa tccccacgcc gttgcggaag gtaaccaacg gagaagataa
     6541 agcaaatgca gatgggaata cagtaagtgc aataattgcg tcactggatt ctcagttccc
     6601 tggcattaaa agtcgtctat gcgatgacaa cggagaatta cgaagctttg taaatatcta
     6661 cgtcaatggg gaagatatta gattcatgga aggggtagga agtggagtga attcgggaga
     6721 cgagataagt atcgtgccgg cagtagctgg aggaagatag taaggattta ctattcgaac
     6781 cgctttacct gatggagtga ttgattagtc cttgctgatt ggtctgcggg ttgataattt
     6841 tcccaaaatg tttccctata ctcctttatt agtccctgct ggattgcctc tcgtaaatca
     6901 gccatggttt gcataagaaa tctcaggttg tgtatcgagg ctaatcgata agccaaatat
     6961 tccttcgcct tgaacaaatg gtgaatatag cctgccgaaa aatgattgca agcataacaa
     7021 tcacaatcag cgtcaaaaga atcatcactt attttgtatt cagatctcgt tacattacgt
     7081 ctaccatatt tagtgtacat cgcacctttc cttgcaaccc gcgtgggaag tacgcagtca
     7141 aacatatcga taccacgtac gacaccctcc accaagtcct ctggcgatcc tacgcccata
     7201 agatatcttg gtttgtcaat tggcaacaat tgggaagtga tttccactgt tgggtacatt
     7261 ttttcctttg tctcccctac actcaatcca ccaatcgcat atcccggcag attaaggctt
     7321 gaaaggaaat cagcagattt ctgcctaagg tcaatatgta gtccgccttg cacaattcca
     7381 aacaaagact ggtcggattt actatccaag gaacgtattg agcgacccaa ccaggtagaa
     7441 gtcaagttca tcgcgtctaa taaatcattt ttagatgccg cagttgggag gcatacatct
     7501 agaggcataa tgacatcaga cccaatgtcg gtttgtattt cgaccacaga ttccggtgtt
     7561 aattcatggg ttgaaccatc aatatgagat ttgaaaataa taccgtcgtc agttatcttt
     7621 cttaaatgtt gtaggctaaa accctgaaat cccccactgt cggtcaagat cggcttgttc
     7681 cagttcataa aagagtgcaa ccctcctaat tcttttattg ttttagaccc aggtcgcaag
     7741 tagagatggt aagcattgcc aagaattatt tgagttccca agcattccaa atctattctg
     7801 tctagagcct tgacagagcc ttgcgtggcg acgggcataa agactggggt acgtatctca
     7861 ccgtgggaag tcttaataca tcctgcccga gcaaatccat cgctatgttc caaagtgaaa
     7921 ttgaacattt ctattttctt taatgcgctg atggcagatt caataaacct ggccataatc
     7981 cttctaaaag taactgtaat cccaaaagta aaaccactaa aagaagtaat ttcagaatcc
     8041 aaaaactctt gattctctgc gctagtcttg cgccaatctg ccccccgaga accaatccta
     8101 atccacccca aaccaatgtt ggataccact ctatattatg attgaaagca tgcgctgcgg
     8161 caccaatcgt ggcataaaat gatagcgaga aaatagaagt agccactgcc acttttactg
     8221 gaaaggaaaa agcggacacc aaaatggggg tcctcaaaaa accgccacct gtgccaaaaa
     8281 aactagatac aaatcctagg atcaaattaa atatagttgc catggattca ttgaaacgat
     8341 agttgaatat cgtcccatca aacgctgtta ttctcctttc tttgacagta aatgacatcc
     8401 atttaggaag ggcaggtttt aaggttatat catctgaggt atagccggaa tcgtcctctg
     8461 ccttgctacc cctaaagaac atgtgaatag ccaatgacaa tagtaaaacc ccgaaaagtg
     8521 tcctgaaaat attacctggc attacatcca atatgaaagg ggctagtaca gaacccggaa
     8581 cagcagcaat ggcaaataaa aacccactcc tcacgtcaat aaaaccagat tttctgtatg
     8641 ctataaaccc ggaaatgcta ttgatggata ctaatgccaa ggaagtacca gcagctattt
     8701 cagggtcttt tgaaaaaaaa agtatcaata ctggagctaa aatgaatccc cctccagccc
     8761 ccaccaaaac cccgtaaatt ccggtagcta caccaagtgt aaaaagaatt gtttcaatca
     8821 agtgtatatc t
//
LOCUS       DASCNS010000030         6029 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000293237, whole genome shotgun sequence.
ACCESSION   DASCNS010000030 DASCNS010000000
VERSION     DASCNS010000030.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000293237"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="QF838_01040"
     CDS             complement(<1..374)
                     /locus_tag="QF838_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HJO59312.1"
                     /translation="MAEMLLRDAMSKALQEALDEDDRSFLMGEDIGAYGGAYAVTRGF
                     LEKYGEERVRDTPISESVFVGAGVGSAMIGMRPIVEVMTINFTLVAMDQIINHAAKLS
                     YMSNGQLQVPLIIRTVTGGGGQL"
     gene            complement(377..1375)
                     /gene="pdhA"
                     /locus_tag="QF838_01045"
     CDS             complement(377..1375)
                     /gene="pdhA"
                     /locus_tag="QF838_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HJO59313.1"
                     /translation="MATTSNIENIEKQEALGIFSQMLLIRRFEEEAERLYRKGLVRGF
                     LHTYIGEEAIAVGAIPTLREDDYVIGHYRDHGHALARGIEPKAAMAELCGKSTGCSGG
                     KGGSMHLFDIEKHFMGGHAIVGGQLPLAIGIALGLKLKKLDSVVMCFFGDGAVNEGEF
                     HEAMNIASLWKLPVVFMLENNLYGMGTDVNRSRAGGSSIYGAAEPYMIPAVQIDGMDV
                     MVVRETVTEGVAKVREGQGPVFIESMAYRFHGHSMADPSNYRDNEEVIENRTRDPIDK
                     FKDDCLKAGKIDDNDIALVAEQIDLLVQEAREFAEQSPEPTVEDLYTHIYAEPEGN"
     gene            complement(1389..2771)
                     /gene="lpdA"
                     /locus_tag="QF838_01050"
     CDS             complement(1389..2771)
                     /gene="lpdA"
                     /locus_tag="QF838_01050"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HJO59314.1"
                     /translation="MADFDVVVLGAGPGGYVAAIRAAQLGLKTAIIERDSVGGVCLNW
                     GCIPSKSLLRNAEVVNLVNNAEEFGITFENLTYDFSKAISRSREVVDKLTSGVQYLLK
                     KNQVELIEGTGKLTGPHELEIMENSRKITSENIIIATGARQRDIDSMPIDHHVVLNSR
                     DALNMSEIPEDITIIGAGATGVEFAHIYKSYGAKVTLVEMMDRIIPNEDSEISESLKK
                     SFTEREIDIRVGVAVKSIQASNGTALVTLIAKEEEHLITSNKVLVAVGVQPNTDKIGL
                     EKVGITTNNGFISISENMETNVSGVYAIGDVTGKMLLAHVASAQAVTAVEYIAGLNPA
                     PLDYQLMPRAIYCEPQVASFGMTEAQAKDNGIETQIGRFPFSASGKAIALGHTEGIVK
                     LVIDPEIGEILGAHMIGPEVTELLAELSMTKLLEGTTTELGWLVHPHPTISETLKEAA
                     LDSTNEAIHI"
     gene            3029..3101
                     /locus_tag="QF838_01055"
     tRNA            3029..3101
                     /locus_tag="QF838_01055"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3062..3064,aa:Phe,seq:gaa)
     gene            3142..3216
                     /locus_tag="QF838_01060"
     tRNA            3142..3216
                     /locus_tag="QF838_01060"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3174..3176,aa:Gly,seq:gcc)
     gene            complement(3227..3643)
                     /locus_tag="QF838_01065"
     CDS             complement(3227..3643)
                     /locus_tag="QF838_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013920.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HJO59315.1"
                     /translation="MTSAYDKPIPIPSNEDLTKPFWEATKRGELIIPYCNSCSNLFFY
                     PREVCPNCFSKDLTWKKMSGKGHVYTYTHVRQAVHPSFQSANRHIYAIVQLDEGPKIP
                     SGITGCEPEEVYVDMPVSVVFDDITEDYTLVKFQKS"
     gene            complement(3733..4845)
                     /locus_tag="QF838_01070"
     CDS             complement(3733..4845)
                     /locus_tag="QF838_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HJO59316.1"
                     /translation="MSVRGRGAIVGIGEVPTRRIYEGRSMYGLCAEAAGLAIADAGIS
                     KSDIDGLVTDGVALPPAIAEYLDIKPNFATGVSMQGASGATATMVATSAVSSGICNTV
                     LVVMGNSRQDAERAGRPAGRGASGPSVRSEWEEPHGMAPGANTGYGLMYQRHIHEYGT
                     TEEQLAKMAADQRFNALENENAVFNGQPISAEDVLNSRYINEPLKLLESVMPCDGAAA
                     LIITTPERAKNMKNTPAYVLGVGLKQGEANIWQRDKFTETPAVDSVRRALEMAGYTPS
                     DIEFAEFYDCYTILAAMCIEDAGLVEKGEIGPFYEETDTTYKGAFPINTDGGQLSGGQ
                     PGLAGGFRHVIEGARQIMGKAGSRQVEKSDLCLVNG"
     gene            complement(4874..5680)
                     /locus_tag="QF838_01075"
     CDS             complement(4874..5680)
                     /locus_tag="QF838_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HJO59317.1"
                     /translation="MGLRSGDGSPSIYNRDRVIIIVAIVIITCVSWMYLFNLSSDMDE
                     MSSLESTTGLSRAMPNMVPWGFSDWISMFVMWFVMMVGMMVPTASPMILMFSTVNQRK
                     KEQNQPFVATAIFLSGYVLVWLGFSIGATATNWILHSNTLLSGIMGESTNNLFGGVLL
                     ISAGIFQWTPIKKACLNNCRTPMGFLMTEWKNGFYGALKMGLNHGIYCLGCCWLLMAL
                     LFVLGVMNLLWIAVLTTIVLFEKVAPKGTWVSRLTGIGFAVWGVMLLFQI"
     gene            complement(5698..>6029)
                     /locus_tag="QF838_01080"
     CDS             complement(5698..>6029)
                     /locus_tag="QF838_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018712.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HJO59318.1"
                     /translation="QIFSGQGGGHFEVLSGFIGEILGVASASIDYRSDGKKRSVTIGG
                     IAEIEIEAIPGVEEEDVTIAGNPLGVVPGVPLHIAQSSVSKFSDHGLTWDNAGNNGYF
                     SAFSYEN"
BASE COUNT         1502 a         1536 c         1195 g         1796 t
ORIGIN      
        1 agttgacctc cgcctccagt aactgttcgt ataataagtg gaacttgcag ttgaccatta
       61 gacatataac ttaattttgc cgcatggttt attatttgat ccattgctac cagcgtgaaa
      121 tttatcgtca tcacctccac tattggtcgc atgccaatca ttgccgaacc gactccggcg
      181 ccgacaaata ctgattcaga tatcggggta tctcttactc tctcttctcc gtatttttcc
      241 aaaaaccctc gggtaaccgc ataagcccct ccataggcgc ctatgtcttc acccataagg
      301 aacgatctgt catcctcgtc gagagcttct tgcaaagctt tcgacatagc atctctcaat
      361 agcatctctg ccatctctaa tttccctccg gttccgcgta aatgtgggta tagaggtctt
      421 caacggtagg ctctgggctt tgttcagcaa attctctcgc ctcttgaact aggagatcta
      481 tctgctctgc tacaagtgcg atatcattat cgtctatctt gcccgccttt aggcaatcgt
      541 ctttaaattt gtcaattgga tcacgggttc tgttttctat aacttcttcg ttatctctgt
      601 aatttgatgg atcggccatt gaatgtccat gaaatctata ggccatggat tcaatgaata
      661 ctggaccttg cccttcccgc acttttgcga caccttccgt gactgtttct ctgaccacca
      721 tcacatccat tccgtctatt tgaacggctg ggatcatata aggttcagcg gccccgtaga
      781 tactcgaacc accggctcga gaacgattga catctgtacc catgccataa agattgtttt
      841 ccagcataaa caccactgga agtttccata atgaggctat gttcatcgct tcatggaatt
      901 caccttcatt caccgcccca tctccaaaga agcacatcac gacactatct aattttttta
      961 atttcagtcc aagtgctatg ccgattgcca atggaagttg gcccccaacg atcgcatgac
     1021 cgcccatgaa atgtttttcg atatcgaata agtgcataga gccacccttg ccaccactgc
     1081 atccagtaga tttcccacat aactcggcca tggcggcctt tggttctatt cctctcgcta
     1141 gcgcgtggcc gtggtctcta taatgaccta ttacgtaatc atcttcccgc agggttggta
     1201 tagctcctac cgctatcgcc tcttcaccta tatatgtatg taagaaaccc cttacaagac
     1261 cctttctata caagcgttca gcttcctctt caaaacgtct tattaaaagc atttggctga
     1321 aaatacccaa cgcttcttgt ttttcaatgt tttctatatt tgaggtagtg gccactttca
     1381 attcttcttt atatgtggat cgcttcgttg gttgagtcta aagccgcttc cttcaaagtc
     1441 tcagatattg ttgggtgggg gtgaactaac caaccaagct ctgtggttgt tccctccaat
     1501 aattttgtca ttgataattc cgcaaggagt tctgtcactt ccggccctat catatgagcc
     1561 ccgagtatct ctccgatttc cgggtctatg actaatttaa caattccctc tgtgtggccg
     1621 agagcaatag ctttgccact agcagaaaaa gggaatcttc ctatttgggt ttcgatacca
     1681 ttgtctttgg cctgggcttc ggtcatccca aaactggcga cttgaggttc acaatatatc
     1741 gcccttggca ttaattgata gtcaagtggt gcaggattta atcccgctat atattcaaca
     1801 gcggtcacag cttgagcaga ggccacgtgt gccaaaagca tcttcccggt cacatcccca
     1861 atggcgtaca cacctgagac gttggtttcc atattttcac taattgagat gaagccgtta
     1921 ttggtggtta ttcccacctt ctccagacct attttatctg tgttgggttg gactcctacc
     1981 gcaactagta ctttgttgga agtgattaaa tgttcttctt cttttgctat taacgtgacc
     2041 aaagctgttc catttgaagc ctgtatagat tttacagcta cccctactct tatatctatt
     2101 tctctttctg tgaaagattt ttttagggac tcacttattt cagagtcttc gttagggata
     2161 attctgtcca tcatttcaac taaggtcact ttcgcaccat agcttttata tatgtgtgcg
     2221 aattctaccc ccgtggcacc cgcacctatt atcgtaatat cttcaggtat ttccgacatg
     2281 tttaacgcgt cgcggctatt caatactacg tgatggtcaa tcggcataga atcgatatct
     2341 ctttgtctag cgccggttgc tattattata ttttcagagg taatttttcg actattctcc
     2401 atgatttcta gctcatgggg cccagttaat tttccggttc cttcaataag ttcaacctgg
     2461 ttttttttca acaaatattg aaccccggag gtcagcttat ccaccacttc tctgctgcgt
     2521 gagatagcct tagaaaaatc gtatgttaaa ttttcgaagg taattccgaa ttcttcggcg
     2581 ttgttaacca agttaacaac ttcggcgttt ctgagcagcg atttgcttgg tatacagccc
     2641 caattaagac aaaccccgcc aacggaatct ctttctataa ttgctgtttt taagccaagc
     2701 tgggccgctc ttatggcagc cacatacccc ccagggcctg cacctaaaac aactacgtca
     2761 aaatcagcca aatcatcacc tctcagagcc ttaagcattt tggtcaacta attatagtca
     2821 tgaaatgcct aaaaatgctg ccctaagtaa ttgagcggct attgtacacc attagctgac
     2881 tggtggctga ctgggcaagg taatgctaat taccctccgc ccaaacataa gaaaatgtgt
     2941 ttgggcggag ggtaattaaa tcacttttaa ttaaattcct tgacactgat aattctcggc
     3001 aataggatgt aatctcggct tgatatgggc cgaggtagct cagtcggtag agcacagcac
     3061 tgaaaatgct ggtgtcgcca gttcgattct ggccctcggc acgccctaag gcccagatct
     3121 gtattgattt tgcggtgact agcggaagtg gctcagtggt agagcatctc cttgccaagg
     3181 agaaggtcgc gggttcgaat cccgtcttcc gctccaaatt taactttcag gacttttgga
     3241 atttgactag agtgtaatcc tcagttatgt cgtcaaagac caccgaaacg ggcatatcca
     3301 cgtacacttc ttccggttca caccccgtta tgcctgaagg aattttaggg ccttcatcca
     3361 actggactat ggcataaata tgacggtttg ctgattgaaa ggatgggtga accgcctgcc
     3421 tgacatgggt gtaagtgtac acatgcccct taccagacat ctttttccaa gtaagatcct
     3481 tggaaaagca gtttgggcaa acttctctag ggtagaagaa aaggtttgaa catgaattgc
     3541 aatacgggat aatcaattcg ccgcgcttcg tagcttccca aaaaggcttg gtcaaatcct
     3601 cattggatgg tatgggtatt ggcttatcgt atgctgaagt catcttaatt aaacctcgat
     3661 tttgttttta atggattaga gtgtgttctc ggacccgaga actaacgagc acatttcgct
     3721 cgctgtgccc cctcaaccat tcaccaggca taagtcactt ttctctacct gtcttgatcc
     3781 agccttgccc attatttgtc gtgcaccctc tatcacatgt ctgaatccac cagccaaacc
     3841 tggttgccca ccagaaagtt gaccaccgtc cgtgtttatt gggaacgcac ctttatatgt
     3901 tgtgtctgtt tcttcgtaaa agggacctat ttcccctttt tctacaaggc cggcatcttc
     3961 aatacacata gctgccagta tggtgtagca gtcgtagaat tccgcgaatt ctatgtcgct
     4021 tggggtgtaa ccggccattt ctaaggctct ccttacggag tcaactgctg gggtttctgt
     4081 aaatttatcc cgttgccaaa tattggcctc accttgcttc agtcctacgc caagtacata
     4141 tgcaggggta tttttcatgt ttttggcccg ttcaggagta gttattatca aggccgcagc
     4201 accgtcacac ggcatgacag actccaacag ctttaagggc tcgttaatat atcttgaatt
     4261 taaaacatct tcagcgctta tgggttggcc attaaaaaca gcattttcat tctctagggc
     4321 attgaatctt tggtctgctg ccattttggc tagttgctct tcagttgtgc catactcatg
     4381 gatatgccgt tgatacatta gaccgtaacc agtattggct cctggagcca tgccatgagg
     4441 ttcttcccac tcagatctca cactgggacc agaggctccg cggccggctg gccgtccggc
     4501 cctttcagca tcctgtcggg aattccccat caccaccaaa acggtgttac aaattccgct
     4561 actgactgca gaggtcgcta ccattgtagc cgtcgccccg ctggcacctt gcattgaaac
     4621 tcccgtcgca aaattaggtt taatgtccaa atattcagct attgcaggtg ggagtgccac
     4681 tccatcggtc actagaccgt ctatatcact tttagagatg ccggcatctg ctattgccaa
     4741 gccagcggct tccgcacata gtccgtacat gcttctccct tcataaatgc ggcgagtcgg
     4801 cacttcccca ataccaacta tggctcctct tcctctgacg ctcatgtatc ttcctcctat
     4861 aattcaaccg tggttaaatt tgaaatagca gcataactcc ccaaactgca aaccctattc
     4921 ctgtgagtcg agacacccag gttcctttgg gcgctacttt ttcaaaaagg actatagtag
     4981 tcagtactgc aatccaaagt aggttcatta cacctagcac gaataataat gccattagca
     5041 accaacagca acctaaacaa tagatgccat ggttgagacc cattttgaga gctccataaa
     5101 agccgttttt ccattcagtc atcaaaaacc ccatcggagt tctgcaattg ttcagacaag
     5161 ctttttttat cggggtccat tgaaaaatcc cagccgaaat taacaagaca cctccgaata
     5221 aattgtttgt tgactcaccc attattccgg ataggagggt attggaatgt agaatccagt
     5281 tggtagctgt agctccgatg gaaaaaccta gccagactaa aacatatccg gaaagaaaaa
     5341 tagcagtcgc cacaaacggc tgattttgtt ccttctttcg ttgattgact gttgaaaaca
     5401 ttagaatcat aggggaggcg gttggtacca tcatgcccac catcattaca aaccacatca
     5461 caaacattga tatccagtcg gaaaatcccc acggcaccat gtttggcata gcccgtgaca
     5521 atcctgtcgt gctttctagg cttgacatct catccatatc actggatagg ttaaatagat
     5581 acatccacga cacacaggtt attatcacga ttgcgacaat aattattacc ctgtcacgat
     5641 tgtaaatcga cggcgagccg tcacccgacc tcaggcccat cacagcaagc ctttaatcta
     5701 gttttcgtaa ctaaatgcgg aaaaatatcc gttgttaccc gcgttatccc aggttaaacc
     5761 gtggtcgctg aatttactca cactggattg ggctatatgt agaggaaccc caggaactac
     5821 acctaagggg ttgccggcta ttgtcacgtc ttcctcctca actcccggta tggcttctat
     5881 ctctatttcc gcgatgccgc caatggtaac tgaacgtttt tttccgtccg atcggtaatc
     5941 tatggaagcc gatgctaccc ctagaatctc tccaataaat cctgaaagta cttcaaaatg
     6001 tccaccacct tggccgctaa atatttgac
//
LOCUS       DASCNS010000031        22155 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000294416, whole genome shotgun sequence.
ACCESSION   DASCNS010000031 DASCNS010000000
VERSION     DASCNS010000031.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 22155)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22155)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22155
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000294416"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="QF838_01085"
     CDS             complement(<1..599)
                     /locus_tag="QF838_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJO59319.1"
                     /translation="MNKDKFITGNINPNRRFRKIIGGCVYILFLLSTCVGIIGLGLLI
                     FDVLKEGIPWINWQFLTDFPSRDPKEAGLLSALFGTIWLMFLTALFTVPIGVGAAIYL
                     EEYAPKNLLTRVIEINVSNLAGVPSIVYGLLGMGIFVYRFGSDEMFLDRVLIAGALTL
                     SLLVLPIVILASRESIRAVPDSYRQGAYAMGADKWQVIKG"
     gene            complement(601..1569)
                     /gene="pstC"
                     /locus_tag="QF838_01090"
     CDS             complement(601..1569)
                     /gene="pstC"
                     /locus_tag="QF838_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HJO59320.1"
                     /translation="MDGAIDDAPHEIPPKETITEVSGLMDPKKKLWVKLEGSIVRWLF
                     MSCALVSILTTVGILIILLYQGYGFFKEINLWEFLTGTRWTPILKPRSYGVLPLVSGT
                     LIVTVIAASVALPVGLMTAIFLSEYAADRVRRVIKPILEILAGIPTVVYGYFALTFVT
                     PLLQNVLDDLLIFNALSAGLVMGIMIIPMVSSLSEDAMISVPRNLREAAYALGATRYE
                     VSLRVVVPAALSGIIASFIIALSRAIGETMLVTIAAGATPKMTLNPLESVQTMTAFIA
                     QTSMGEQAVGSFEYNTIFAVGLLLFCMTLLMNLLGFWIVRRFREAY"
     gene            complement(1634..2611)
                     /locus_tag="QF838_01095"
     CDS             complement(1634..2611)
                     /locus_tag="QF838_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007127416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HJO59321.1"
                     /translation="MFTLESITQLGRGLVITIGLSLISIMMSCGSSSSEELVAPATEP
                     ASKLKGTIEIDGSSTVYPISEAVAEEFNKIFPKVRVNVGVSGTGGGFKRFTVGETDIS
                     DASRLMKNPEETSKAEENEVTYDELRLGTDGLSIMVNPDNNFVDCLTVEELKAIWNPE
                     STITNWNQVQSGFPDQKIRLYGPDSDSGTFDYFTEEINGEAQASRADYTASADDNVLV
                     QGIAGDKGSLGYFGYAYYAENKDKLKLVAVDSGSGCVEPSLETIPSGKYTPLSRPLFI
                     YVNEKSYQRPEVKAFVNFYMEQGPNLTNEVGYIASGNATYTDNKDKLAK"
     gene            complement(2939..4303)
                     /locus_tag="QF838_01100"
     CDS             complement(2939..4303)
                     /locus_tag="QF838_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJO59322.1"
                     /translation="MTFVVIFSSVYIFNRLDEVYSGQASELVVVVILVGLLTTFAALL
                     VILYTSRRINRAINKLITGVSLISEGKLDHRVTSISKDETSDLADAFNHMAETISGMV
                     IALSSERNTLSALLDTMGDGVIVTDENGTITLLNKTAQAIFNLENSPYLGLRLVDVIR
                     DFEIIRMASDCFEGKESIQDEIEIPEIRRYLSVTTTPVGSGSEAGVLITVHDLTNIRQ
                     LENTRKEFVSNVSHELRSPLASVKAMIGTLENGAYEDPRIAGDFLKRIDRDVDRMTSL
                     VADLLELSRLESGQENIELEPVEVRSLVAETISIILDQIENCPAISSNIIDTVEVLGD
                     KQKITQILINLLQNSAKFTSHEGKVTVSAKSDEEFLEISVQDTGSGIGTEHLPHLFER
                     FYKVDRSRRDGGTGLGLAIVKHIVQAHGGVVYVDSIEGEGSNFHFTLPLLNRLHVVRN
                     FGSN"
     gene            complement(4386..5075)
                     /locus_tag="QF838_01105"
     CDS             complement(4386..5075)
                     /locus_tag="QF838_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HJO59323.1"
                     /translation="MSSEKILIIEDEENILEAVKYSLTQEGYDVFTSIDGEEGLEKAQ
                     EIRPDLVLLDIMLPKMDGFEVCRILRKNLDMPVFMISAKIEEIDRVVGLEIGADDYLT
                     KPFSMRELVARVRNVLRRAALNPVENNLEDIKYDELEIHLTSHRVIVRGEEISMKPKE
                     FDLLYFLASHKGRAFTRDQILEKLWGNEFIGDIRTVDVHVRWIREKIEANPSQPKKLV
                     TIRGLGYRFDE"
     gene            complement(5269..6378)
                     /locus_tag="QF838_01110"
     CDS             complement(5269..6378)
                     /locus_tag="QF838_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HJO59324.1"
                     /translation="MLDRFKVPKEDIVKVDHESLQETVTQIFGKMGVVPEDAKEGASV
                     LVSTDLRGVETHGVSNMLRSYVERYNAGDLKPRPDWKIERETQGTATIDADNGLAVIL
                     GPKAMRIAIEKAKTVGVGVVTMHNAGHSGAIGHHAMLAAKEDMIGMTATAGGMSVLPT
                     FGAEPRLGTNPIAIAAPARNNPPFLFDAATSAIAGNKLGLAARVGANLLPNWISQMDG
                     SPIGDEVPMPDRGDFHQLPLGGTREQGSHKGYGFGLMSEILATGLSGAISAMVIGSGK
                     TSRTGGTSCHYFCAYNIEAFTDVELFKDNMDNMLQALKDTPPAPGQDRVIYPGLSEYE
                     EEEDRKANGIPLHSEVVEWFTDITSELSVPMVKIL"
     gene            complement(6411..7541)
                     /locus_tag="QF838_01115"
     CDS             complement(6411..7541)
                     /locus_tag="QF838_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015780.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJO59325.1"
                     /translation="MYSPGKDILESLGLSPLISATGTVTAYGGSRLRPEVMDAMNRAS
                     NTMVNMDELNLAVGKQIAALTGAESGMVTSGAAGGLILQAAAVIAGNDPIKMKKLPDA
                     NGMKNEIVIHRSHRFPYDQCYLSVGAKFVEIGDGRRCQPWELEGSLTENTAAVAYLFS
                     PFITRRALPFEYVCEVAHSKGIPVIVDAASYLPPRANITRFIKEGADMVVYSGGKGIR
                     GPQGTGVLCGRSDLIEAAYANASPHQFIGRGLKVAKEEIIGLSKALEIFLSEDEEKEN
                     ERYQQMCDQIVDALIEIPGVGLQTKFDPYDYLIPTALITFDPNRNGLSRDQVWEQMVA
                     GDPPIYLSNLGTPDELAVDPFNIADSELEIVIARLRELLIQN"
     gene            complement(7606..8634)
                     /gene="queA"
                     /locus_tag="QF838_01120"
     CDS             complement(7606..8634)
                     /gene="queA"
                     /locus_tag="QF838_01120"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HJO59326.1"
                     /translation="MKTADFDYKLPEKLIAQSPLEPRDHSRLMILDRINGRIKHRNFY
                     DITEYLDHGDVLVFNDSKVIPARMYGSTVDSGSRVELLLLSQISSNVWKTLVKPGRRM
                     RKGAKFIINGINGKQLIGRILKEHLDGSREVEFEGELNFEYFGSLPLPPYIHETAVPG
                     DRYQTVYAEKEGSIAAPTAGLHFTKKLLNRISEKGVLLEFVTLHVGWDSFRPLKTNNP
                     LEHKMHSEYWELTREACDRINLAKSEGRRVISVGTTAVRLLENAARISLSSTLSPGSG
                     WADIFIKPGYQFRVVDSLITNFHLPKSTLLMLTSALAGRDNILKAYEEAVQNQYRFYS
                     FGDAMFIV"
     gene            8786..9658
                     /locus_tag="QF838_01125"
     CDS             8786..9658
                     /locus_tag="QF838_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59327.1"
                     /translation="MTQFYPEDKWIVANDLNIHYLDWGGDKTKKPLILMHGIGGHAHL
                     FDDISSEFFDKWHVVAIDARGCGDSDWSRGGYSVQNFASDVAAFAKVTGLYPFDYYGH
                     SQGSRIGIALGAYYGDFISHLALGDYGPMPDPSPAGKATANQRMTKGAKEKPRGFFTP
                     EQAFDWHKESEPTLTDEQIWQTVKYTYRTNWDGILIPKTDPEMKWLLGRTALKEGEFL
                     WDCVPKISCSTLIIRGETSTVLDLNQANEMAAKIPSNKGIVKEIDGAGHGLHSENREG
                     TVKALREFFSGQNN"
     gene            9670..10581
                     /locus_tag="QF838_01130"
     CDS             9670..10581
                     /locus_tag="QF838_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HJO59328.1"
                     /translation="MSLNNIINFLYEAVTDFLSKNCVFMAAAISFYALFSMFPLGLAV
                     VSVMAFIIGPDSDRTELAIQMAEVIPVSSELIGSTMEGIVKARTITGIASFLGLIWAS
                     SAAFGAIRRGINTAWGVTRPRPFLRERLIDIGLVFGAGILVLIILLTAPIISVVIDLF
                     GYFDPDGDFRIDFVLDLIAQLISPILAFISILILYAWMPNVKVRMQYVIPGALIATFG
                     FIAGQWCFIWVVSTFSLYNAVYGSVGALLALQAWLYISAIILLFGAQLTSMFHKYGVA
                     EDEKSKLDTMFLGLKRRGNLIVRSIKP"
     gene            10559..11656
                     /locus_tag="QF838_01135"
     CDS             10559..11656
                     /locus_tag="QF838_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HJO59329.1"
                     /translation="MFVRLNLKKVRPLLLLITILATHACSADKTPYSVIDSNDFTQKI
                     SSGNIHLNKSPIGTQNLFPLADLVETVQQSVVSISVDIVSRGRFFDFTDEGSGTGIII
                     SNDGYIITNYHVIQNTSDIEVSLADGRNYSAEIVGLDVLTDLAVIKIEADNLSTIKFG
                     DSSGLRPGDWVFTIGNALGLRGGPSVTFGIISGVGRTVQTERGDLYDMIQTDAAINQG
                     NSGGPLVSDEGEVIGINTAVYSGAQGIGFSVSSSVAQPVIDSLIKEGKVTRPLIGLRG
                     INTTNTIAKRYDLGVSKGIFVTHISPNGPADHGNLKVGDVITAINGTATPTMSEFLPL
                     LWGYNVGDIVTVTYISEKSPLMTEIVLDVRP"
     gene            complement(11658..12374)
                     /locus_tag="QF838_01140"
     CDS             complement(11658..12374)
                     /locus_tag="QF838_01140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HJO59330.1"
                     /translation="MVQLKGNNRSAFVASMFGRISRRYDLLNTLMTVGMHYIWRRKAA
                     KLAATHVTGDILDVASGTGDFAFDLLKHSNVKIVVGLDFSPEMLDIAIGKSVRLGFEG
                     SFIPVMGDAHFLPFPDERFVSTTVGFGARNFSDLPKALADIVRVTRRGGKVTVLEIVR
                     PQGKLASVLFLGYFGWVTPKLGRFFAGDYEAYNYLPESVQNFMSATQLSEILEGAGLR
                     NLVVETKGMGSIAIISGKRP"
     gene            complement(12382..13938)
                     /locus_tag="QF838_01145"
     CDS             complement(12382..13938)
                     /locus_tag="QF838_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HJO59331.1"
                     /translation="MKILSSHQMRDLEAKSETLGISTNELMNNAGFSIASHIAKNMGP
                     LKGLKVVSLVGTGNNGSDCLIASGHLAKWGASVTALILKPRAEPDLRSQETLESGISL
                     IDIGEGKGNISFSSVHSLLKNAHIIIDGILGIGTSLPIRDPFKTVLSDIRDLNGKKAK
                     VFSIDVPSGVDSDTSAVDPLAVKPDVTLALGYLKPCHANQPAANFCGEIIICDIGLPS
                     HLSGEMDTELLSDDYVRPILPARPISSHKGSYGKGMVVGGSDNYIGAPVLAASSAMKT
                     GLGLVTIATFEGLVNPMANMFPEATFLPLKEAEMDIGSGQHMARQIFEAVEDYKVLLI
                     GCGFGTSRTADRIFQNLVLSNIKLPQLVLDADGLNMLSKISSWWNKIPFNSILTPHPK
                     EMSRLTRLSVSEIQSDRLNVAKRFSEQWGVTVVLKGANTVICDPNGSSTISPYANPIL
                     SSAGTGDVLAGLITGFIGQGNSPLEASSLGVYIHGKTADSVSLQIGSSGLLATELANN
                     VPFTIKYLRN"
     gene            complement(13952..14671)
                     /locus_tag="QF838_01150"
     CDS             complement(13952..14671)
                     /locus_tag="QF838_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015956.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HJO59332.1"
                     /translation="MANSRAITSAVFNTSRLSMAMENYLLSILRLEEQQTKVSVAQLS
                     EHFKTLPLGEGLGTSLPSVSAMIRRMSREKLVGISTDKEIVLTKNGRQYAEVILRRHR
                     LAERLLVDVLNIDLEFAHIEAHRLEHAMSPYLEQKIVDLLGSPQTCPFGHPIPGSDYT
                     VPANAVTLDKCRPGNKYVLDRVPEDNQDLLAYFVEKGFVPETSIKIIDSSLPRGIVTL
                     ECNQDELVFSTVVAKLIWVKS"
     gene            complement(14721..15119)
                     /gene="rpsI"
                     /locus_tag="QF838_01155"
     CDS             complement(14721..15119)
                     /gene="rpsI"
                     /locus_tag="QF838_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HJO59333.1"
                     /translation="MVEQQYTYAVGRRKTSVAQVRLYQDAGPVVVEDKLIEDAFPFST
                     WQQIINEPFTVTNTQGKFRVVAKVKGGGISGRAGAIRHGISRALAEIDNGRYRDELKR
                     AGLLTRDSRVKERKKPGLKGARKAKQYTKR"
     gene            complement(15157..15591)
                     /gene="rplM"
                     /locus_tag="QF838_01160"
     CDS             complement(15157..15591)
                     /gene="rplM"
                     /locus_tag="QF838_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HJO59334.1"
                     /translation="MTIKQKMEQPNISELKPGWHVIDAEGKTLGRISSEIAILLQGKH
                     KPGYVPYLNTGDYVVVVNAEKIHVTGKKVEQKKYYRHSGYHGGLTERTLQQMLDKHPD
                     RVIKQSVKGMLPKNIPGRRMLGRLKVYPGSEHPHQAQINSAE"
     gene            complement(15603..16361)
                     /gene="truA"
                     /locus_tag="QF838_01165"
     CDS             complement(15603..16361)
                     /gene="truA"
                     /locus_tag="QF838_01165"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HJO59335.1"
                     /translation="MKLALTIEYQGTGYHGFQYQTNANTVQEELENSIERLTGRKTRI
                     TAAGRTDAGVHARNQIATFDTNSDLETDQIAKGMNHYLPDDIVVKKVVQVGKKFDPRR
                     DAKSRVYRYSILNSNFRSPLKRLYTHSVIGSLDRDLMCEGAELFLGEHDFSKFAGKVE
                     NNKTTVRMIYRSEWTYGHETLTYEVEGNAFLPHQVRRMVGSLVDLGRGNLKLQDVQDL
                     INGEGSARSNAMPSKGLCLEKIIYENYCDLTPLN"
     gene            complement(16383..16736)
                     /gene="rplQ"
                     /locus_tag="QF838_01170"
     CDS             complement(16383..16736)
                     /gene="rplQ"
                     /locus_tag="QF838_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HJO59336.1"
                     /translation="MRHQLSGRKLGRPTGHRLLMLRNMSTDLLRNERMKTTDSKAKEV
                     RKMAEKIITLGKKGSLHHRRQAAGLLTDEAVVKKVFDELGDRYQDRNGGYIRITKLGP
                     RKGDASEMAILELVE"
     gene            complement(16755..17804)
                     /locus_tag="QF838_01175"
     CDS             complement(16755..17804)
                     /locus_tag="QF838_01175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HJO59337.1"
                     /translation="MVTDFTAMSILDAPEEEEEAIVANPRISVAEASETYGKFVIGPL
                     DPGYGVTLGNPLRRVLYNSLSGSAVTWVKIEGTLHEYSNIDNVKEEVSDILLNVKNLR
                     IRTESESVGQLRLVTRGEGKITAGDITPSSEITVVNTDQHIATLDSADAELDIVLNVD
                     HGKGYKVADSSEGHPIGVIPIDAVFTPVRKVNYTINQTRVGQRTDFEELHLEVWTDGS
                     KYPVEAVIEAANILVNQFFLFTKAEDAQEGSDGVSMDIPAEHYNMTVEELELSSRTLN
                     CLKRAALNKVGEVMEKSKGELLEIRNFGEKSYRELYDRFRELEILPEHLDPELNANQK
                     EQIAESNPEIEVDEG"
     gene            complement(17823..18422)
                     /gene="rpsD"
                     /locus_tag="QF838_01180"
     CDS             complement(17823..18422)
                     /gene="rpsD"
                     /locus_tag="QF838_01180"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HJO59338.1"
                     /translation="MARYTGPIQKKLRSLGLDNFGAYKVRKENKSNQGFTRRRRVSAY
                     GLQLKEKQKARFLYGIMEKQFKNYYEKALYMDGITGDNLMVLLEKRLDNALYRTGMFF
                     SRKQSRQAVNHGHFMVNGAKVDIPSYQVGQGDEISWGPKGQKTELFEIAKSNCKSIQS
                     PHWINLDQDKMTATIVTEPGAEDGEIVIDTVQIVEYYSK"
     gene            complement(18480..18866)
                     /gene="rpsK"
                     /locus_tag="QF838_01185"
     CDS             complement(18480..18866)
                     /gene="rpsK"
                     /locus_tag="QF838_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HJO59339.1"
                     /translation="MARRPRTRRRDRGPVPLGRAYIQSTFNNTIITLTDPTGNTVAWG
                     SAGTAGFRGSRKSTAFAAQRAGEDAAKKAIDEGVRQVDVFVRGPGAGRESAIRAIQGA
                     GIVVSSIKDVTPIPHNGCRPPKRRRV"
     gene            complement(18909..19286)
                     /gene="rpsM"
                     /locus_tag="QF838_01190"
     CDS             complement(18909..19286)
                     /gene="rpsM"
                     /locus_tag="QF838_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HJO59340.1"
                     /translation="MAIVAGVNIPDAKRVETALTSIYGIGPAVALDVLNQAGIIDNPR
                     VRDLSDEDLNRVREIIDKGRLVEGDLRREVNQNIRRLIDIGCYRGTRHRRGLPARGQR
                     TKTNARAKRGRKMAVSRKHSTTR"
     gene            complement(19296..19409)
                     /gene="rpmJ"
                     /locus_tag="QF838_01195"
     CDS             complement(19296..19409)
                     /gene="rpmJ"
                     /locus_tag="QF838_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HJO59341.1"
                     /translation="MKVSASVKVRCAKCRVIRRKGRVRIICENPKHKQRQG"
     gene            complement(19460..19684)
                     /gene="infA"
                     /locus_tag="QF838_01200"
     CDS             complement(19460..19684)
                     /gene="infA"
                     /locus_tag="QF838_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HJO59342.1"
                     /translation="MTKKEKESIEVEGTVLEALPNASFKVELANGHEVLAYISGKLRV
                     NYIRVLPGDRVLVDLSPYDLTRGRITYRFR"
     gene            complement(19716..20501)
                     /gene="map"
                     /locus_tag="QF838_01205"
     CDS             complement(19716..20501)
                     /gene="map"
                     /locus_tag="QF838_01205"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HJO59343.1"
                     /translation="MTAGNANTKGINVKSPREINLMREAGRIIAEAKSAVKDALAPGV
                     TSAEMDALAEEIIKKRGAIPSFKGYQPSPHMPPFPATICFSFNEEIVHGIPSDRKMRD
                     GDIVSVDFGAIVEGYHGDSAFTSGVGLVKDTAQKLIDVTEESLFAGIAKAMPGNRLTD
                     ISNAIQVYAEAEGFSIVREYVGHGIGRALHEEPQIPNYGSPGRGPLLQEGMVLAIEPM
                     LNIGTWKTKIMPDGWTVITEDGELSAHSEHTVVINSEGPEVLT"
     gene            complement(20570..21208)
                     /locus_tag="QF838_01210"
     CDS             complement(20570..21208)
                     /locus_tag="QF838_01210"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016645164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HJO59344.1"
                     /translation="MKIILMGPPGSGKGTQASFVASLLQVNSVSSGDLFRENLANNTE
                     LGKLAKTYMDQGKYVPDDVTINMVLSWIENPVNNGGFILDGFPRTLSQAQALDDRLGE
                     VAGIDKVILFKVPELELIKRLSGRILCRKCQTPYHKEFSPPTNDGTCDKCGGDLFQRD
                     DDSEVVVKERLKVYRQETDPVVERYKEIGNILEIDGSLPIGVVEKSLRKALG"
     gene            complement(21242..>22155)
                     /locus_tag="QF838_01215"
     CDS             complement(21242..>22155)
                     /locus_tag="QF838_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HJO59345.1"
                     /translation="WGQLTLLRSSGVLPGVDFGLNLHTFAMMLSLVAGTMFLVWLGEL
                     ITERGLGNGVSLIIFGGIVAGLPQMLGRGFLENDYVSTLIMLGIIGFALIYIIVFFNE
                     AQRRVPVQYGRSVFRGGRMQRQSGSSFLPIRVNTAGMIPLIFAFSIIILPGTIASYFN
                     DPLSDGFLNSIASFFASTLDPSNLPYWIAVFILVVMFTFFYTLVIFQQQNLAETLQRN
                     GGFIPGIRPGLPTQEYLNRVIIRITWGGALFLGGIAIVPYFVSELTGIQALTLSSTSL
                     LIMVGVALDTMRQLESQLLMRNYEGFL"
BASE COUNT         6246 a         5286 c         3936 g         6687 t
ORIGIN      
        1 cctttaatga cctgccattt gtctgccccc atagcatagg ccccctgacg gtatgaatca
       61 ggaacagcac gaatagattc tctcgaagca agaataacga ttggaagaac cagcaaggat
      121 aaagttaaag caccggctat cagaacccta tccaaaaaca tttcgtcact cccaaaacga
      181 tagacgaaaa tccccatacc caggagacca taaactatgg aaggtactcc tgctaagttt
      241 gaaacattta tctcaatgac cctggtaagg agattctttg gcgcatattc ttccagatat
      301 atggcggctc ccaccccgat aggtacggtg aatagcgccg tcagaaacat taaccatatg
      361 gttccaaaca aggcggataa aagaccagct tccttagggt ctcttgaagg gaaatccgtc
      421 aaaaattgcc agtttatcca aggtattcct tcttttaaaa catcaaagat taaaaggcct
      481 aatccaataa ttcccacaca agttgaaaga agaaacagta tatatacaca acctccaatt
      541 atttttcgga accttctatt aggatttata ttccctgtaa taaatttgtc tttgttcata
      601 tcagtaagcc tcccgaaacc ttcttacgat ccagaatcca agtaggttca tcaaaagcgt
      661 catacaaaac aaaagtagcc caaccgcaaa tattgtgttg tactcaaaag agcccacagc
      721 ttgctctccc atactagttt gggctatgaa agccgtcatt gtctgcacac tctccaatgg
      781 atttaaagtc attttaggag tagctccagc agctatggtt actagcattg tctcaccaat
      841 tgctctcgat aacgcaatga tgaaagaagc tattattcca gagagagcag cgggaactac
      901 taccctaaga ctgacctcat atcttgtggc tcccaatgca taagcggcct ctctcaaatt
      961 ccttggcact gaaatcatgg catcttcact gagggaagaa accatcggta tgatcataat
     1021 gcccatcacg agccctgcac tcaacgcatt aaatatcagt aaatcatcca gtacattttg
     1081 taaaagtggc gtgacaaatg taagggcaaa atacccataa acaacagttg gtataccagc
     1141 gagaatctcc aatatcggct taataaccct cctaactcta tccgccgcat attctgacaa
     1201 aaatatagca gtcataagac caacaggcag agctactgaa gcggcaatca cagttactat
     1261 taaggttcct gaaacgagag gcaaaacccc gtatgaccta ggctttaata ttggagtcca
     1321 tcgagtacct gtgagaaatt cccaaaggtt tatttcctta aaaaacccat agccttgata
     1381 caacaagatt ataagaatgc ccactgtcgt caatattgac accaatgcac atgacataaa
     1441 taaccatcta actatagatc cttcaagttt tacccataac ttttttttag gatccatgag
     1501 ccctgacact tcggttatag tctcctttgg cggtatttca tgtggggcat catctatagc
     1561 tccatccatt atggccccct actcccaagc ctacaaatat aacttgaaaa caactaccgt
     1621 aggagatcct tagttacttc gctaacttat ctttgttgtc agtataagtc gcatttcccg
     1681 aggctatata tccgacttca ttagtgaggt ttgggccctg ctccatgtag aaatttacaa
     1741 aagctttaac ttctggccgt tggtagcttt tctcatttac ataaatgaac agtggtcgcg
     1801 acagtggtgt gtatttaccg gagggtattg tttccaggct tggttctaca cagcctgatc
     1861 cactatcaac ggcaacaagc ttcagcttat ctttgttttc agcatagtaa gcatacccaa
     1921 aataacctaa agaacctttg tcacctgcga tgccctgcac cagaacattg tcatctgcac
     1981 tggcagtgta atcagctcta gaagcctgag cctctccatt tatttcttcc gtgaagtaat
     2041 caaaggtccc ggagtcactg tcaggtccat acaaccttat cttctgatca ggaaacccac
     2101 tctgtacctg attccaattt gtaatcgttg actcaggatt ccaaattgct tttaattcct
     2161 caactgtcaa acaatcaaca aagttattat cgggattaac cattatggaa agtccatccg
     2221 taccaagtct gagctcatca taagtaacct cgttttcctc agctttacta gtttcttcag
     2281 ggtttttcat tagacgagaa gcatcagata tatctgtttc cccaactgta aacctcttaa
     2341 accctccccc tgtaccagaa acccctacat ttaccctaac tttcggaaaa attttgttaa
     2401 attcttctgc tacagcctct gaaatcggat aaacagtaga agaaccgtct atctcaatcg
     2461 tcccttttaa cttagatgcc ggctcagttg ctggagctac aagctcttct gaagaactac
     2521 ttccacaaga catcattatt gaaattaagg ataaccctat tgtgattact aaacccctac
     2581 ccagctgggt aatgctttct agagtaaaca tcaatttata taacacccgt aatattcaca
     2641 atattcttct tatgttttca aataattatt taacattttt aaatatatct acacataagt
     2701 tgaccttgat gtacgaggct tgttttagga gaatcactta aacttaaaat atctttagtc
     2761 tttgatttat attcaaggta aaaatcctgc cttcataaat ctgaatattt tgagtttaat
     2821 tcgaaccgag cgtctcgtgc cgataattta cttacaaata aatgttagtg gactaagacg
     2881 aggctgagtt taacgaaagg ttaacgaaaa attaaaatac ggttattaag aagaggtctt
     2941 aattactacc gaaattccta accacgtgaa gccggttcaa taatggcagg gtaaagtgaa
     3001 aattactccc ctcaccttca atgctgtcaa catagaccac tccgccgtga gcctggacta
     3061 tatgcttaac aatggccaag ccgagcccag taccaccatc tcttcgagac cgatcaactt
     3121 tatagaaccg ttcaaacaaa tgaggtaagt gttcagtccc aataccggaa ccagtatctt
     3181 gcactgaaat ttctaaaaat tcctcatcag acttagcact tactgttacc ttgccttcat
     3241 gtgaagtgaa ttttgcagaa ttctgaagca aattaatcag tatttgagta atcttctgtt
     3301 tatccccaag tacctccact gtatctatta tattggaaga tatggcggga caattttcaa
     3361 tctgatctaa aattatggaa atagtctcag ctactaaaga tcttacttcg acaggctcga
     3421 gttcaatatt ttcctgacca ctttctagtc tagaaagctc cagtaaatct gctaccaaag
     3481 aggtcatcct atcaacgtcc ctatcgattc ttttgaggaa atcaccagca attcttggat
     3541 cttcgtatgc tccattctca agggttccaa tcatagcttt taccgaagct agcggactcc
     3601 tgagttcatg agacacattg ctcacgaatt ctttgcgggt attttctaac tgccgaatat
     3661 ttgttaaatc atgaacagtt attaataccc ctgcctctga acctgaaccc accggtgttg
     3721 tagtcacgct tagataacgt ctaatttctg gtatctcaat ctcatcttgt attgactctt
     3781 tcccttcaaa acaatcagag gccatccgga ttatttcgaa atccctgatc acgtcaacta
     3841 atcgcaaacc caaatatggt gaattttcta aattaaaaat agcttgggca gtcttattta
     3901 gtaaagtaat cgttccattt tcatcagtaa cgatcacccc atcccccata gtgtccagaa
     3961 gggcagacaa agtgtttctt tctgaggaaa gagcaataac catcccagat atagtttccg
     4021 ccatatggtt aaaagcatcc gctaaatctg aggtttcatc ctttgaaatt gaagtgaccc
     4081 tatggtctaa cttcccttca gaaatcaagg aaacccccgt aattaattta tttatcgctc
     4141 ggttgatcct tctactggta taaagaatta ccagcaacgc ggcaaacgtc gtcagtaggc
     4201 caaccagtat cacaacgact actaattcgc tagcctgacc tgagtaaacc tcgtccagcc
     4261 gattaaatat atagacactc gagaaaatga cgacgaaagt caccaaaatt aaaaaacctg
     4321 aaattatttt ccatagaggt gaactaaaaa agcttaacac agtcatcccc tggcaaatct
     4381 attacctact cgtcgaaccg gtaccctagt cctcttattg ttaccaattt tttaggctga
     4441 ctggggttcg cctcaatttt ctctcgaatc catcttacgt gaacatctac ggttctaatg
     4501 tctccaataa attcattacc ccacaatttt tccagaatct gatctcttgt aaaagctctg
     4561 cctttatgtg aagccaaaaa atagagcaaa tcaaattctt tgggcttcat agaaatttcc
     4621 tcacctctga ctatgactct gtgactggtt aagtgaattt ccaattcatc atattttata
     4681 tcttcaagat tattttctac cggatttaaa gccgccctcc tgagaacgtt tctaactctg
     4741 gcaacaagtt ccctcatgct aaaaggttta gttagataat cgtcggctcc aatttcaaga
     4801 ccgacaaccc gatcgatctc ctcaatcttg gctgagatca taaaaactgg catatccaaa
     4861 ttcttacgaa gaattctgca aacctcaaat ccatccattt tgggcagcat aatatccaac
     4921 aacactaaat cgggcctgat ctcttgagcc ttctctaagc cctcttctcc atcaattgag
     4981 gtaaatacat cgtaaccttc ttgagttaag ctatatttca ccgcctccaa aatattttct
     5041 tcatcttcaa taatgagaat tttttcgctg ctcactccaa atctccctgc tatttgtgtg
     5101 atcctcttaa ttaattacta atatcgattc atcaaatatg taagtctcta aataagatta
     5161 cgaagtaaga tgtacattgg atttaaaaaa aatccggaat caaccctaca ttgaatattt
     5221 cctttgctca tcgcatattg gtgcaaaatc acaaaaggac tgtcccagtt acaatatttt
     5281 gaccataggc acagaaagtt cgctggttat atctgtaaac cactccacca cttcagagtg
     5341 aagagggata ccatttgctt ttctatcctc ttcttcctcg tattcagaaa gcccggggta
     5401 tatgacccta tcttgcccag gggcaggtgg agtatccttc aaagcctgaa gcatgttatc
     5461 catgttatct ttgaacaatt caacatctgt aaatgcttct atattgtatg cacaaaaata
     5521 gtggcaactt gttcctccag tcctgctagt tttacccgaa ccgatgacca tagcacttat
     5581 agctccactc aatcccgtag ctaatatttc tgacatcaaa ccaaaaccat aacctttgtg
     5641 ggaaccttgt tctcttgttc cccctagagg gagttgatga aaatctccac gatccggcat
     5701 tggaacttcg tcccctatgg gagacccgtc catctgtgat atccagttag gaagtagatt
     5761 ggctcctaca cgagctgcta gccctagttt attacctgct atagcagagg tggcggcgtc
     5821 aaaaaggaat ggtggattat tcctcgctgg agctgctatc gctatcgggt tagtacctaa
     5881 tctaggctct gcaccgaacg ttggaagtac actcatccct ccagcggtag ccgtcatgcc
     5941 tatcatgtct tccttagccg ctagcattgc gtgatgacca attgcgccgg aatgtccagc
     6001 attgtgcatc gtcacaaccc ctactccaac agttttcgcc ttctcgatgg caatcctcat
     6061 tgccttaggg cccaatataa ccgccaaacc attatccgca tcgatcgtcg cggtaccttg
     6121 ggtctctcgt tcaatcttcc aatctggtcg aggtttcaaa tctcctgcat tgtatctttc
     6181 tacataactc cgaagcatgt tagatactcc gtgtgtttcc actcctcgaa gatctgttga
     6241 tacaagaaca ctagcccctt ccttggcatc ttctggtacg actcccattt ttccaaaaat
     6301 ttgagttaca gtttcctgca aagattcatg atctacttta acgatatctt cttttggaac
     6361 tttaaatcga tctaacatgt tttcctaacc tccgtcatcc caagatgaaa ctaattttga
     6421 atcagtaatt cccgtagtct agcaataaca atttccaatt ctgaatcggc aatgttgaaa
     6481 ggatccactg ctagttcgtc gggtgtgccc aaattgctga gatatatagg cgggtctcct
     6541 gctaccatct gttcccaaac ctgatctcgt gacaggccat ttctattggg gtcaaatgta
     6601 attaaagcag taggtattag ataatcatat ggatcaaatt tagtctgcag cccgacacct
     6661 ggaatttcta tcaaggcatc cacaatttgg tcacacattt gctgatacct ttcgttttct
     6721 ttttcctcat cttcagacag aaatatttct aatgcttttg ataaacctat aatttcttct
     6781 ttcgcaacct tcaaacctct acctataaat tggtgggggc tcgcgttggc atatgcagcc
     6841 tcaatgagat ctgacctacc acatagcaca ccggtacctt gtgggcccct tattcccttg
     6901 ccaccactgt aaacaaccat atcagcccct tcttttataa agcgggtaat gttggctcta
     6961 ggaggcaaat aggaagcggc gtctacaata acaggaatcc ctttagaatg agctacttca
     7021 catacatatt caaaaggcaa ggctcttctg gttataaacg gtgaaaacaa atatgcaaca
     7081 gcagctgtgt tttcagtgag actaccttcc agctcccacg gctggcatct acgaccatct
     7141 ccaatttcta caaatttggc ccccacagac aggtaacatt ggtcataggg aaatctatgg
     7201 cttctatgta tgacaatttc atttttcatg ccatttgcat ctggtagttt tttcatctta
     7261 atagggtcat taccagcaat taccgctgct gcctgcagaa taagaccgcc agctgcacca
     7321 ctagtgacca ttcccgactc tgcaccggtt aaagccgcta tttgtttacc aacagctagg
     7381 ttaagttcat ccatattgac catagtattg gaggcccgat tcatagcatc cattacttcc
     7441 ggtctaagcc tactaccacc ataagcagtc actgtaccag tagcagatat aaggggactg
     7501 agacctagag attctaatat gtctttacct ggagaataca ttaggaaaac ctcacacaat
     7561 tcaataacgg catgtaccgc ggattataca ctgcccattt cgagtttaaa ctatgaacat
     7621 tgcatcacca aaactataaa acctatattg gttctgaacc gcttcttcat acgcctttaa
     7681 aatattatcc cgaccggcaa gggcactcgt taacattagc aaagtagatt taggcaaatg
     7741 gaaatttgtg attaaactat ccacaacgcg aaattgatat cctggtttaa taaaaatgtc
     7801 agcccatcca gaacctggac ttaaagtaga agacaaactg attcttgccg cattttccaa
     7861 aagccgtacc gccgtggtcc ctacagatat aaccctccgc ccttcacttt tcgccagatt
     7921 tattctgtca cacgcctcgc gggtaagttc ccaatattct gaatgcattt tatgttctaa
     7981 aggattgttg gtttttagag gcctgaaaga atcccaacca acatgaaggg ttacaaattc
     8041 tagcaacact cccttctcgg atatccggtt aagtaatttt tttgtgaagt gaagaccagc
     8101 ggtcggggcc gcaatacttc cttctttttc tgcgtataca gtttggtatc ggtcaccagg
     8161 tactgctgtt tcatgtatgt aaggagggag cggcagggac ccaaaatact caaagtttaa
     8221 ctcaccctcg aactcaactt ctcgactacc atcaagatgt tccttcagaa ttcttccaat
     8281 taattgtttc ccattaattc catttattat aaattttgcc ccttttctca ttcttcgtcc
     8341 tggtttaact aaggttttcc aaacattaga actgatctga gatagcaata ggagttcgac
     8401 tcggctaccg ctatctaccg ttgatccata cattcttgct ggtatgacct tactatcatt
     8461 aaagaccaac acatccccgt ggtctaaata ttcagtaata tcataaaaat tacggtgttt
     8521 aattcgacca tttattcgat ccagtatcat aaggcgtgag tgatctcttg gttctaaagg
     8581 agattgggca atcaattttt ctggaagttt gtagtcaaaa tcagcagtct tcaaatttag
     8641 ttcctacaga aatatgatgt ttattcaatt ctataattct acaagagcaa ttcgtttcaa
     8701 aagtaaggtt aggctcccat tattggtaga ataggttcgc cagtttcaac gagtgttgga
     8761 caagtttaaa taagaggtgt tatccatgac ccaattttat cctgaagaca aatggatcgt
     8821 tgcaaatgat ctcaatattc actaccttga ctggggtggc gacaaaacaa agaaacctct
     8881 aatactgatg catggtattg gtggtcatgc tcatcttttt gatgacatat catctgaatt
     8941 ttttgacaag tggcacgttg tagccataga tgctcgaggt tgcggcgata gcgattggag
     9001 cagaggcggt tactcggttc aaaacttcgc ttccgacgta gccgcatttg ccaaagttac
     9061 cggtctatac ccatttgatt attacggcca ttcccaagga tcccgaatag gcatagcttt
     9121 aggagcatat tatggggatt ttatttcgca cctggcattg ggagattatg ggccaatgcc
     9181 ggacccttct cctgccggta aagccacagc taaccaaagg atgacaaagg gggcgaaaga
     9241 aaaacctaga ggctttttca cacctgaaca agcatttgat tggcataagg aatctgaacc
     9301 taccttaacc gacgaacaaa tatggcaaac tgttaagtac acctatcgca ccaattggga
     9361 tggaatactc atacctaaaa ccgatccaga gatgaagtgg cttttaggta gaactgctct
     9421 taaagagggg gaattcctgt gggattgtgt gcccaaaata tcctgcagca cactaattat
     9481 cagaggagaa acctccaccg tactagacct taaccaagcc aatgaaatgg cggctaagat
     9541 tccttctaat aaagggattg ttaaagaaat agatggcgcc ggacacggcc ttcacagtga
     9601 aaatcgcgaa ggaacggtaa aggcattgag ggaatttttc tcaggacaaa acaattaaga
     9661 atcatatcga tgtctctcaa taacatcata aatttccttt atgaagctgt taccgatttt
     9721 ttatccaaaa attgtgtctt tatggcggcg gcgatttctt tctacgcatt gttttcaatg
     9781 tttccactag gtttagctgt ggtatctgta atggcattca tcattggccc agacagtgac
     9841 agaaccgagt tagctatcca gatggcagaa gttatacccg tttcaagtga attgattggt
     9901 tcgaccatgg aaggtatagt gaaagctagg actatcacag gaattgccag ttttcttgga
     9961 ttgatatggg cttcgtctgc tgcttttggg gccattagaa gaggaatcaa tacagcctgg
    10021 ggagtaacac gtcccaggcc tttcctccgt gaaagattga tagatatcgg cttagttttc
    10081 ggtgcaggaa tactggtgtt aataatcctt ttgactgcgc ctatcatctc cgttgtaata
    10141 gatctcttcg gatattttga ccctgacgga gatttccgta tagattttgt ccttgactta
    10201 atagctcaat taatttcacc aatattggct ttcatatcta tactaatact ttatgcatgg
    10261 atgccaaatg taaaagtgag gatgcagtat gtgatacccg gggctcttat tgccactttt
    10321 gggtttattg ctgggcagtg gtgtttcata tgggtagtaa gtacgttctc cctttacaac
    10381 gcagtttacg gatccgtagg agcacttttg gctttacaag catggcttta tatctccgcc
    10441 atcatattac tttttggggc gcaacttaca tcaatgtttc ataaatatgg tgttgcagaa
    10501 gatgaaaaaa gcaaattaga tacaatgttc cttggcttga aaagaagggg taatttgatt
    10561 gttcgttcga ttaaacctta aaaaggtacg ccctctcctc ttattaatca ccattttggc
    10621 aactcatgct tgttctgctg ataagactcc ctattctgtc attgattcga acgactttac
    10681 ccagaaaatt tcatctggaa atattcatct aaataaaagt cccatcggta cacaaaattt
    10741 attccccttg gccgatttag ttgagaccgt ccaacaatct gttgtatcca tatccgtaga
    10801 catagtttca aggggacggt tctttgattt tactgatgag ggatctggaa ctggcataat
    10861 cattagtaat gatggctaca ttattaccaa ttatcatgtg atacaaaata catctgatat
    10921 agaagtatct ctagcagatg gcagaaacta ttcagctgaa atagtaggtc ttgatgtttt
    10981 aactgatctc gccgtaatca aaatagaagc ggacaatctt tctacaatta aatttggaga
    11041 ctccagtggg cttagacccg gagactgggt atttacgatt gggaatgcat taggtttgag
    11101 gggtggccca agcgtgacat tcggcatcat aagtggtgtc ggaagaacag ttcagactga
    11161 aagaggggat ttatacgaca tgatccaaac tgatgccgcc atcaatcaag gtaacagtgg
    11221 tggtccattg gtcagcgacg agggggaagt cattggtata aatactgctg tatacagtgg
    11281 tgcccaagga atagggtttt cagtctcttc ctcggtggca cagccggtga tcgatagtct
    11341 tataaaagaa ggaaaagtaa cccggccatt aataggactc cgtggaataa atacaaccaa
    11401 tacaattgca aaaaggtatg acttaggtgt ctcaaaaggt atattcgtta cccacatatc
    11461 gccgaatggt cccgccgatc acggaaacct taaagtaggg gatgtcatca cagcaattaa
    11521 cggaaccgca actccaacta tgtcagagtt cttacccctc ctttggggtt ataacgtagg
    11581 cgatatagta acggtgacct atatttccga aaaatctccc ttaatgacag aaatagtctt
    11641 agatgtaagg ccttaattta aggacgtttc cccgaaatta tagcgattga acccatccct
    11701 ttagtctcga caaccaagtt tctcaaccct gcaccctcta aaatttcaga aagctgcgtg
    11761 gctgacataa aattctgaac agattccgga agataattat aagcttcgta atccccagcg
    11821 aaaaatcttc ccagcttagg agtcacccac ccgaaataac ctaaaaacaa aacggatgcc
    11881 aatttacctt gaggcctaac gatttccaac actgtaactt taccaccccg ccgagttact
    11941 cttacaatgt ctgccagtgc cttcggtaga tcagaaaaat ttcgtgcccc aaatccaaca
    12001 gtagtactaa caaacctttc atcgggaaat ggcaaaaaat gtgcgtcgcc catcacagga
    12061 ataaagctac cttcaaaccc taatctaaca cttttgccta tagcgatatc taacatttcg
    12121 ggagaaaaat ccaatccaac aacaatcttg acattggagt gcttaagtag gtcaaaggca
    12181 aaatcaccag tgccagaagc tacatctaaa atatcccctg tgacatgagt tgcagctaat
    12241 tttgccgcct ttcttctcca tatgtaatgc attccaactg tcatgagagt atttagcagg
    12301 tcatatcggc gtgaaattct tccaaacatt gatgccacaa aagctgatct attattccct
    12361 tttagctgaa ccaaagtatc tctaattcct caaatattta atcgtaaatg gaacattatt
    12421 cgctaattca gttgcaagta accctgaaga tccaatctga agtgacactg agtcagcagt
    12481 tttaccatga atgtaaactc ccaacgaaga agcttctaac ggactattgc cctgtccgat
    12541 gaaccctgta atcaaccctg ccaacacgtc acctgtacca gcagaagata aaataggatt
    12601 tgcataagga cttattgttg aagaaccatt tggatcacat atcacagtat tggcaccctt
    12661 taaaactaca gtgactcccc attgttctga aaatctcttc gccacattta aacgatccga
    12721 ctgtatttcc gatactgata gcctggttaa acgtgacatt tccttgggat ggggggttaa
    12781 tatactgttg aaaggtatct tgttccacca gctcgaaatt ttcgacaaca tattcaaacc
    12841 gtcggcatct aaaacaagct gaggaagttt aatgttagac agaactaggt tctgaaaaat
    12901 cctatccgct gttcgcgaag ttccaaagcc acatccaatt aataaaactt tatagtcttc
    12961 aactgcttca aaaatttgcc ttgccatatg ttgaccggac cctatatcca tttcggcctc
    13021 tttgagaggt agaaatgtag cttcaggaaa catattagcc ataggattta ctaaaccctc
    13081 aaaggttgcg atagtcacaa ggccaagtcc tgtcttcata gctgatgaag ctgcaagaac
    13141 cggtgcacca atgtagttat ctgacccacc tactaccatc ccttttccgt aactaccttt
    13201 gtgggatgaa atcggtcggg ctggcaaaat tggtcttaca taatcatccg acaacaattc
    13261 ggtatccatc tcaccactta aatgactggg taaaccaata tcacagataa ttatttcccc
    13321 acaaaaattt gctgcaggct ggttagcatg acaaggcttt aaatagccaa gtgcaagggt
    13381 tacatccggt ttcacagcca acggatccac tgcagaggtg tcagaatcta caccagatgg
    13441 gacgtctata gaaaaaacct tcgctttttt tccatttaaa tccctaatat cagaaagaac
    13501 tgttttaaag ggatcacgaa taggtaaact ggtcccaatc cctaaaatgc catcgattat
    13561 gatgtgggca ttttttaaaa gtgaatgcac tgaggaaaaa gagatatttc ctttcccttc
    13621 acctatatct attaatgaaa ttcccgactc gagagtttcc tggcttctta agtcgggttc
    13681 tgcgcgtggt ttgagaatca atgcagtaac tgaagctccc cacttagcta agtggccact
    13741 agctattaag caatccgatc cattgtttcc tgtccccacg agcgaaacca ctttcaagcc
    13801 tttcagcggc cccatatttt ttgctatgtg gcttgctatt gagaatccag cattattcat
    13861 taattcattg gtggatattc caagtgtttc actcttagct tcaagatccc gcatttgatg
    13921 agatgaaagg attttcaaaa atttctactt gttaggactt aacccatata agtttggcca
    13981 ctacagtact aaacacaagt tcatcttgat tgcattcgag ggttacgatc cctcttggca
    14041 aagatgaatc gattatctta atggaagtct ctggaacaaa tcctttttca acaaaatagg
    14101 ctaacaaatc ctgattatcc tcaggaactc tgtccaaaac atatttgttg ccaggtctac
    14161 acttatctaa cgttacagcg ttagctggaa cggtatagtc actccctggt atcggatgtc
    14221 caaaaggaca agtctgaggg ctacctaata agtctacaat cttttgttct aagtatggag
    14281 acatagcgtg ttccagtcga tgtgcctcaa tgtgagcaaa ctctaagtcg atatttaata
    14341 catccaccaa aagtctttca gccagacggt gtcttcgtaa aataacttcc gcatactgac
    14401 ggccgttttt agtcaggaca atttctttgt ctgtggagat accaaccagt ttttctctgg
    14461 acatccttct gatcattgca ctgacagaag gaagtgatgt acctagtcct tcacccagtg
    14521 gcaaagtttt aaaatgctca gacaattggg caacggaaac tttggtctgt tgttcctcaa
    14581 ggcgaagaat agacaaaaga tagttttcca ttgccatgga taatctagaa gtattaaaaa
    14641 cagctgacgt tatggctctt gaatttgcca atctccgcac ctatatcgat agcaccaaga
    14701 aactcaaatt atagtttaag ttaacgctta gtgtactgtt tggcctttcg tgctccctta
    14761 agaccaggtt tttttcgttc ttttacacgt gaatctctgg taagtaaccc tgcccttttc
    14821 agctcatctc tgtatctgcc attgtcaatt tcagcaagag cccttgatat accgtgcctt
    14881 atggctccag cccgtccgct aattccacca cccttcacct tagccacgac cctaaatttc
    14941 ccttgggtat ttgttacagt gaaaggctca tttattattt gttgccatgt actaaaaggg
    15001 aaagcatctt ctattaattt atcttccaca actacaggac cagcatcctg atatagccta
    15061 acctgcgcca ctgaggtctt tctcctacca actgcatatg tgtattgttg ttcaaccaat
    15121 gttttctcct ttccaaatta ggcaattaac ccttcgttat tcagcagaat ttatttgggc
    15181 ctgatgagga tgttcacttc cgggataaac tttcaatcgg cccaacatcc tccttcccgg
    15241 aatgtttttg ggcagcatcc ctttcacaga ctgtttaata acacggtcag gatgtttatc
    15301 cagcatttgc tgtaaagttc tttccgttaa accaccatgg tatccactgt gtcgatagta
    15361 tttcttttgc tctactttct tccctgtaac atgaattttt tccgcattta ctacaactac
    15421 atagtcccca gtgttcaaat agggaacata tccaggttta tgcttgccct gcaaaagtat
    15481 cgcaatttcc gagcttatcc tacccagtgt tttgccttcc gcatcaatta catgccaacc
    15541 tggtttcaat tcacttatat taggctgttc cattttttgt tttatcgtca taggtatatc
    15601 cttcaattga gaggagttaa atcacaatag ttctcataaa ttattttttc gagacacaat
    15661 ccttttgaag gcattgcatt gctcctagca gaaccttcac cgttaattag atcttggaca
    15721 tcctgcaatt ttaagtttcc cctacccaag tccaccaaac ttcctaccat ccgtctcact
    15781 tggtgaggga gaaaggcatt tccttcaact tcataggtaa gcgtttcatg cccataagtc
    15841 cattcagatc gataaatcat tctgacagtg gttttgttat tttccacctt accggcaaat
    15901 ttactaaaat catgttctcc caaaaacaac tcagctccct cacacataag gtctctatca
    15961 agactgccga taacactatg tgtgtacaac ctcttcaaag gcgaacgaaa attggaatta
    16021 agaatcgaat atctgtaaac ccttgatttc gcgtctctcc taggatcaaa tttctttcct
    16081 acttgaacaa cttttttaac aacaatgtcg tctggaaggt aatgattcat tcctttggct
    16141 atctggtccg tttccaaatc ggagtttgtg tcgaatgttg cgatttggtt tctagcatga
    16201 actcctgcgt ccgttctacc tgctgcagta attcttgttt ttctaccggt gagccgttct
    16261 attgagtttt ctaattcctc ctgtacagtg tttgcgttag tttgatattg aaatccatga
    16321 tatccggtcc cttgatattc aattgtaagc gccagtttca tgttacttgg aaagtcttgt
    16381 gcctattcaa ccaactctaa aatagccatt tccgaggcgt cccctttcct tgggcccaat
    16441 ttagttattc tgatgtaacc accattccga tcttgatagc gatctccaag ctcatcaaat
    16501 acctttttca cgacagcctc atctgtgagt aagccggctg cttgtcgcct gtgatgcaag
    16561 ctacctttct tccccaaagt tataatcttc tcagccatct ttctgacttc tttagcctta
    16621 gaatctgtag ttttcatcct ctcatttcgt aacagatcag tcgacatatt cctaagcatg
    16681 agaagtctat gcccggtagg cctacctaat tttcttcctg aaagttgatg tctcacgatg
    16741 aatatctcct attcctatcc ttcatcaact tcgatttcag gattgctttc tgcgatttgt
    16801 tctttctggt tggcattcaa ttccggatct agatgttctg gcagtatttc tagctctcta
    16861 aacctgtcat aaagttccct ataggacttt tctccgaaat tccttatctc caacaattca
    16921 ccctttgact tttccataac ttcgccaacc ttattaaggg ccgcgcgctt gaggcaattc
    16981 aaagttctag atgaaagttc taactcctcc actgtcatgt tgtaatgctc tgccggtata
    17041 tccattgata ctccatcaga gccttcttgg gcatcctccg ctttggtgaa tagaaaaaac
    17101 tgattcacca atatatttgc agcctctata actgcctcca ccggatattt tgacccatct
    17161 gtccatactt ccaggtgaag ttcttcgaag tcagtcctct ggcctactct agtctggttt
    17221 atggtgtaat taacctttct cactggagta aatacggcgt ctataggaat tacccctatg
    17281 gggtgtcctt cactgctgtc ggctacttta tagcctttcc catgatcaac attcaagact
    17341 atatctagtt ccgcatcggc agagtctaag gtggcaatat gctgatctgt atttacgaca
    17401 gtgatttctg aagaaggcgt gatatcccct gccgtaatct taccctctcc tctggtcact
    17461 aatctaagtt gtcctacgga ctctgattcg gttcgtatcc tcagattctt tacattgagc
    17521 aatatatctg aaacctcttc cttcacgttg tcaatatttg aatactcatg cagagtaccc
    17581 tctattttta cccatgtaac agcggatcca gataaggagt tgtataaaac tctcctcaaa
    17641 ggattcccta acgttacgcc gtagccaggg tctaatgggc caattacaaa tttcccatac
    17701 gtttcagatg cttccgccac cgatatccgt ggattggcga caatagcttc ctcttcctct
    17761 tcaggagcat ccaagataga catagcagta aaatccgtta ccatttaaat agcctcctgt
    17821 atttactttg agtaatattc cacaatttga acagtatcga tgactatttc accatcctcc
    17881 gcacctggct cagtgactat tgtggcagtc attttatctt gatccagatt aatccaatga
    17941 ggactttgga tagatttgca attgcttttt gctatctcga acagttctgt cttctgaccc
    18001 tttggccccc acgaaatttc gtctccctgt cctacctgat aagaaggaat atctactttg
    18061 gctccattta ccatgaagtg accatggttg acggcttgcc ttgattgttt tcttgaaaag
    18121 aacatgccgg tcctatacag ggcattgtct agacgtttct caagcaacac catgagatta
    18181 tccccggtta ttccgtccat atataaggct ttttcataat aattcttgaa ctgtttttcc
    18241 ataatcccat ataaaaatct agccttctgt ttttctttta actgcaaccc atatgcagaa
    18301 acacgtcttc tgcgtgtaaa tccttggtta gatttgtttt cttttcgaac tttataggca
    18361 ccaaaattat ctaatcccaa tgatctgagt tttttctgaa tcggtcccgt gtatctcgcc
    18421 atatgtttac gctctatttc tttttgttaa tttcatgttt gggtagtaaa aaatattttc
    18481 taaacccttc ttctcttcgg cggacgacac ccattatggg gtatgggagt aacgtctttt
    18541 atactactaa caactatacc tgctccctgt atggctctaa tcgcagactc tcttcctgct
    18601 cctggtcccc ttacaaaaac atcgacctgt cttacccctt catcgatagc cttcttcgct
    18661 gcatcttctc cagctctttg ggcagcgaaa gctgtcgatt tacgggaacc tcgaaatcct
    18721 gctgttcctg cacttcccca ggcaactgtg ttacctgtcg ggtcagtcaa agttattatt
    18781 gtattattga aggtagattg aatgtacgct ctacccaaag gtacaggtcc tctatctctt
    18841 cttcttgttc tcggccgtct agccattatt taactcctta ggttaccaat gccagtaaga
    18901 aatttcgttt acctagttgt agaatgtttc cttgacacag ccatttttcg gcctctttta
    18961 gcccttgcat tggttttggt cctctgacct cttgcaggta gccctctcct gtgacgtgtt
    19021 cctcgataac acccaatgtc aatcaacctt cgaatgtttt gattgacctc ccttcttaaa
    19081 tctccttcca ctaagcgtcc cttatctatg atttccctca ctcgattcaa atcttcatcc
    19141 gacaagtctc ttactcgagg gttgtcaata attcccgcct ggttaagtac atccaatgcc
    19201 actgctgggc caatcccata gatactggtc agtgcggtct cgacccgttt tgcgtctggt
    19261 atatttactc ctgctactat agccatcagt cacccttatc cttgtctttg cttatgcttg
    19321 gggttttcgc agataatcct tactctacct ttacgacgaa taacccggca cttagcgcac
    19381 ctaactttca cggaagcgct aaccttcata actttctcca ttttgtatta ctatcgcttt
    19441 tctcagatac attgaaaaac tatctaaatc tatatgtgat cctacctctt gttagatcat
    19501 agggagatag gtccaccagc actctatcac caggcaaaac ccgaatgtaa tttaccctca
    19561 acttccccga tatataggct agtacttcat gcccattggc aagttcaact ttaaaagaag
    19621 cattcggcag tgcttctagc actgtacctt ctacttctat tgattccttc tcctttttgg
    19681 tcataaaact cctatagtta gtcttcccgc ccttcttatg tcagaacctc aggaccttca
    19741 gaatttatca ccacagtatg ttctgagtgg gcagataatt ccccatcctc agttatcaca
    19801 gtccagccat ctggcataat tttggttttc catgtgccta tattaagcat cggttcaatc
    19861 gcaagcacca taccttcttg caataaaggc cctctaccgg ggcttccata gttaggaatt
    19921 tgtggttctt catgaagggc tctccctatc ccatgtccca cgtattccct gacgattgaa
    19981 aacccttcag cttcagcata aacctgtatc gcattcgata tatcagtcag ccggttccca
    20041 ggcatagcct ttgctatccc tgcaaataaa gattcctcag tcacatcaat cagtttctga
    20101 gctgtgtcct tcactagccc tacccctgaa gtgaatgcac tatctccatg gtacccttct
    20161 actatcgctc caaaatccac actgactata tcgccatctc tcattttacg atcactcgga
    20221 atgccgtgca caatttcttc gttaaaggag aaacaaatcg tagctggaaa aggcggcata
    20281 tgaggggatg gctgatatcc cttaaatgaa ggaatagctc cccttttttt tattatctcc
    20341 tccgctaaag cgtccatttc tgcgctagtt acccctgggg ccaacgcatc tttcactgct
    20401 gatttggctt cagctataat acgccctgct tctctcatca aattaatctc tcgaggagac
    20461 ttaacattta tccctttggt gttagcattt ccggccgtca taaactaaat tactccaacc
    20521 gtcagcaaaa aagctgaaac actattatac aatgaacgca ccagagtaat catcccaaag
    20581 ctttccttag agatttttca accacgccta ttggaagaga accatctatt tcgagtatat
    20641 tgcctatttc tttatatctt tcaacaactg ggtcagtttc ttgcctgtat accttcagcc
    20701 gctcctttac aacaacctct gaatcgtcat ctcgctggaa taaatcaccg ccacatttat
    20761 cacaagtacc atcgtttgtg ggaggggaga attccttgtg atatggagtt tggcattttc
    20821 tacacaaaat tcttcccgac aacctcttta tcaactccaa ttctggcact ttgaataata
    20881 ttactttatc aattccggct acctcaccca ggcgatcatc taatgcctgt gcttgcgaca
    20941 gtgtccgagg aaacccatct aaaatgaatc ctccattatt tactggattt tctatccacg
    21001 aaagcaccat atttatggtc acatcgtccg gtacatattt gccttgatcc atataagttt
    21061 tggccaattt tcctaactcg gtgttattgg ctagattctc cctaaataga tctccagacg
    21121 acacactatt aacttgcagg agagaagcta cgaatgaagc ctgagtacct ttaccagaac
    21181 ccggtgggcc cataaggatt attttcatac cctctcgcct ttgtactggt acatgtgaac
    21241 cctataaaaa tccttcatag ttacgcatca aaagctgcga ctccaactgc cgcatagtgt
    21301 ccaaggccac cccaaccatg attaacaaac tagtactact caaggttaag gcttgaattc
    21361 cagttagctc agagacaaaa tatgggacta ttgctatacc accgaggaaa agagcccctc
    21421 cccaagtgat cctaatgatc accctattta gatactcttg agtcggtaat cctgggcgta
    21481 ttccgggtat aaacccaccg tttctttgta gggtttcagc aagattttgc tgttgaaaaa
    21541 ttaccagggt atagaaaaag gtaaacatta ctaccaatat aaaaacagct atccaatatg
    21601 gaaggtttga tggatcaagg gtacttgcaa aaaaagacgc gattgagttt aaaaacccat
    21661 cgctcaatgg atcgttgaag tagctagcaa tcgtcccagg caggataatt attgaaaaag
    21721 caaaaatcaa cgggatcata cctgctgtat tcactctgat aggcaaaaaa gaagacccac
    21781 tctgacgttg catccgccct ccacggaaga cactcctgcc atattgcact ggcaccctac
    21841 gctgtgcctc attaaaaaag acgattatat atatcaaggc aaacccaatt attccaagca
    21901 taattagtgt cgatacatag tcattttcaa gaaatcctct tcccagcatt tgtggcagac
    21961 ctgcaacaat gcccccaaat atgattagag aaaccccgtt accaagacct ctctcggtta
    22021 ttaactcccc tagccacacc agaaacatag tacccgcaac tagagacaac atcatggcaa
    22081 aggtatgtaa atttaggcca aaatcaactc caggaagcac tcctgaggat ctgagcaggg
    22141 ttaactgccc ccaac
//
LOCUS       DASCNS010000032         2530 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000303375, whole genome shotgun sequence.
ACCESSION   DASCNS010000032 DASCNS010000000
VERSION     DASCNS010000032.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000303375"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="QF838_01220"
     CDS             complement(<1..511)
                     /locus_tag="QF838_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59346.1"
                     /translation="MGEIAIALLSKELKFCELSLESSNSTRLEVKVGRNKRSKIPNKN
                     LVYRSGIHVSDTVDPQKFSLVVTEISETLDLHLAWELLTQHLIEKETQEIHVCEIADL
                     LDFEKDPYLIAAIYLHIHKDSLFFSISENRLLIHSPQNVESIKATRIRSNRENEDELT
                     LIDAIKNQSI"
     gene            682..1788
                     /locus_tag="QF838_01225"
     CDS             682..1788
                     /locus_tag="QF838_01225"
                     /inference="COORDINATES: protein motif:HMM:NF017181.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59347.1"
                     /translation="MKNIHALALNILKDLGRCPATSFNEYQVSNKLMSILKENSIDYS
                     QDDYGNIVAKISGTEESHRPIAYVAHMDHPGYEIIREDEDFFVGKSLGGVPRAAAMKG
                     VDCFSFDQENNRHPCRIEPWNDGGEGEVKVVSEIKLDVGTPITFNLPDFSLIDNQIEM
                     RALDDLAGCASIMASLIELNRESVVTDIFGIFTRAEEVGLVGAGLIAAEQTIPSNTFV
                     VSVETSSVIPGVEQGMGPVIRTGDASYTFDAEAENILALAKKSLLAQDPEFKCQRQLM
                     AAGSCEATAFAVNGYSTTGIAFPLGNWHNATTKIPDPEGSVGMEYISQSDFLNGIDLI
                     FTSALVGYSNSSSPIKERLFEVQGKLKDRLRRPI"
     gene            complement(1785..2429)
                     /locus_tag="QF838_01230"
     CDS             complement(1785..2429)
                     /locus_tag="QF838_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59348.1"
                     /translation="MVNPIKPAITYFPEFETLYMLIASEIDGLSEEQLDYTSDVWEWA
                     GWSIRNQVSHMASLIPRWLVLRWGSTLFPLEDHGVENIEEISQSKSDRRLDDEVYWRI
                     EDISLMLKKFIDLAIRVLCDNSLEFMASQKIDRDATPQWEMMAKAHPNGVTSEGIPAK
                     GSMTLEATMRHIYFEEVTHLYNIQRLKKAQGLIPKMVVPNVGYWTLDGWDRSQP"
BASE COUNT          702 a          485 c          548 g          795 t
ORIGIN      
        1 ggatcgattg gttttttata gcgtcaatca aggttagttc gtcttcattc tccctatttg
       61 acctgatcct ggtcgctttt attgattcaa cgttttgagg agaatgtatc aataatctat
      121 tttcggatat tgaaaaaaat aatgaatctt tgtgaatatg aagataaata gctgcgatta
      181 agtaggggtc tttctcaaaa tccagtaaat cagctatttc gcaaacatga atttcctggg
      241 tttctttttc aatcaaatgt tgagttagca gttcccaggc gaggtgtaaa tctaaagttt
      301 cagatatctc ggttaccact aaggaaaatt tttgtgggtc aacagtatca cttacatgta
      361 tgccagatct gtataccaaa tttttgtttg gtattttaga tcgtttattc ctgcccactt
      421 ttacttcaag gcgggtgcta ttagaggatt ccaaggacaa ttcacagaac tttagttctt
      481 tactgagtaa ggctatagct atttcaccca tagagctcct tcgaattcag tatctagaaa
      541 agatgttacg gtgtttttag atccaacacc ggaaactatg atctgaggat cgttagtaaa
      601 ggtccatata tttgttgtac atttcctact tattgtgcga atcttctatc agtatatgac
      661 acgccatttt tggaggtgta tatgaaaaac attcatgcct tggctttaaa tattctaaaa
      721 gacttgggcc gttgtcctgc aacttccttt aacgagtatc aagtatccaa caaattaatg
      781 tcgattctga aagaaaattc cattgattat tcccaagatg actatggaaa tattgtggcg
      841 aaaatttctg ggaccgaaga gagtcatcga cccatagcat atgtggccca tatggatcat
      901 ccaggttacg aaattattcg ggaggatgaa gatttttttg ttggaaaatc cttgggaggt
      961 gttccaaggg cggctgcaat gaaaggggta gattgctttt cttttgatca ggaaaataat
     1021 agacatccat gccgtattga accgtggaat gatggcggcg aaggggaagt aaaggtagtt
     1081 tcagaaataa aattggatgt tggaactcca ataacgttca atttgccgga ttttagcctg
     1141 atagataacc aaatagaaat gcgggcatta gatgatcttg ccggatgtgc ttcgataatg
     1201 gcttcattaa tcgagttaaa ccgagagtct gtagtgacag atattttcgg gatttttact
     1261 cgggctgagg aggtagggtt ggtaggagct gggctaattg ccgccgagca gactatacca
     1321 tcaaatactt ttgtggtgtc tgttgaaact agctctgtta ttcccggggt ggaacagggc
     1381 atgggaccgg ttatacggac aggagacgct tcctacacct ttgatgccga agctgaaaac
     1441 attttggcat tggcaaaaaa atctttactt gcgcaggatc cagaatttaa atgccagagg
     1501 caattaatgg ctgcaggatc ttgtgaagct actgcttttg cggtgaatgg ttattcgacc
     1561 acagggattg catttccttt gggcaattgg cataacgcga caacgaagat accagatcca
     1621 gaagggagtg taggtatgga atacatatct caatctgatt ttttaaatgg gatagattta
     1681 attttcacgt cagctctcgt gggttattca aactctagct ctccgattaa ggaacggctt
     1741 tttgaagtgc aggggaaatt aaaagatcga cttaggcgac ctatttaagg ttggctgcga
     1801 tcccatccgt ctagggtcca gtatcccaca tttggcacca ccatttttgg gatcaatcct
     1861 tgggcttttt ttaagcgttg aatattgtat aagtgggtta cttcctcaaa ataaatgtgt
     1921 cgcatcgtgg cttcaagggt catagaaccc tttgcgggga tcccttctga agtcacccca
     1981 ttgggatgag cttttgccat catctcccac tggggagtgg catctctatc gattttttga
     2041 gaagccataa actcaagcga attatcacag agtactcgta tagccaaatc aatgaacttc
     2101 ttcagcataa ggcttatgtc ttcgattctc caataaactt cgtcatcaag acgcctatct
     2161 gatttcgatt gggatatctc ttcaatattt tcgactccat ggtcttctag agggaaaagg
     2221 gttgaccccc accttaaaac tagccacctt ggaattaacg atgccatgtg gctgacttga
     2281 ttgcgaatgc tccatccggc ccattcccaa acatctgaag tgtaatccaa ctgctcttca
     2341 gacagaccat caatctcgct tgcaatcaac atatagaggg tttcgaattc agggaaataa
     2401 gtaattgctg gcttgatagg attcaccata actaatcttt gtacctttcg atggcacgga
     2461 gcaaatgctc cattgataga tttccaccac ttgctattat gaccactttt ttatttctca
     2521 atctttcttt
//
LOCUS       DASCNS010000033         2253 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000312387, whole genome shotgun sequence.
ACCESSION   DASCNS010000033 DASCNS010000000
VERSION     DASCNS010000033.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000312387"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="QF838_01235"
     CDS             complement(<1..729)
                     /locus_tag="QF838_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013811.3"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HJO59349.1"
                     /translation="MIDFQYLSSIRKQTASKIVMIVVDGLGGMTDPSTGNSELETAVL
                     PNLDKLAANSSCGVSTPVLPGITPGSGPGHMALFGYNPIKYLLGRGVLEGLGIGAPIG
                     KGDVAARGNFCLLGDDGKILDRRAGRITTEECVELIELLKQIEIPGFGIEIYPVMDYR
                     FVLVLKGETSSPDVSDTDPQITGIAPLISSPTSERGTGTALAVNLFTQRSMEILTEHK
                     GSANGILLRGFSNIPELPHFGSSYA"
     gene            complement(736..1935)
                     /locus_tag="QF838_01240"
     CDS             complement(736..1935)
                     /locus_tag="QF838_01240"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HJO59350.1"
                     /translation="MSPKLSIEDVELSGQRALVRVDYNVPFLEGTSIISDDTRIKASI
                     PTLRHLLGKGCSLVLCSHLGRPKGKRYPELSLKPIAKRLSEILEQKVQFVEDCVSEEA
                     AATAWKLSEGEILLLENLRFHREEEENNLGFAEQLSKLAEVFINDAFGAAHRAHASTL
                     GVTRFLPSYAGLLLDKEVNYLENALENPKLPFTAILGGAKVSDKIKVVDRLTKIASHI
                     CIGGGMVATFLAASNKEVGNSSIEADFISQAQTLLNENDSKNGNLTLPSDVVVVKTLL
                     KDSDFLTKSVDDLEDGDIIVDIGPATAEFYSSIIRKSNTVLWNGPMGIFEWEKARAGT
                     RSVAKALANGNMLSIIGGGSTVDAVNFFGLQGQMTHVSTGGGASLELLEGRELPGVTS
                     LREKIEG"
     gene            complement(1939..>2253)
                     /locus_tag="QF838_01245"
     CDS             complement(1939..>2253)
                     /locus_tag="QF838_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59351.1"
                     /translation="QIWGSDSNQKIDFLNKKKLFPVIVALEKATPSQKRKLGDVYFKR
                     VLADSDLDAVLDVLSDLGGKEPAELRYATAKDDALSIVQKNLPNDNEENLIDYLEEII
                     EE"
BASE COUNT          607 a          542 c          421 g          683 t
ORIGIN      
        1 agcatatgat gaaccgaaat gtggcaattc aggtatgttt gagaaaccgc gtagtaaaat
       61 gccgttagca cttcctttgt gttccgtaag aatttccata cttctttggg tgaaaagatt
      121 aaccgccaag gctgtgccag tccctctctc agaggttgga ctcgagatca atggtgcaat
      181 acctgtaatc tgaggatcgg tgtctgaaac atcaggagaa gaggtttctc ctttgagaac
      241 caagacgaac cggtaatcca taactggata tatttcgatt ccgaatccag gaatctcgat
      301 ttgtttgagc aactctatta gttccacgca ttcttcagtt gtaattcttc ctgcacgtct
      361 atctaaaatc ttgccatcat cccctaaaag gcaaaaattt cctcttgcgg ctacgtcccc
      421 cttgcctata ggggccccta ttcctagccc ttccagcact cctcgaccca aaagatactt
      481 aatagggttg tatccaaaca aagccatgtg tccggggccg ctgccagggg ttatgccggg
      541 aaggacaggc gtacttacac cgcaggaact attggcagcc aatttgtcca agtttggcaa
      601 cacagcagtc tctaattcag aattacctgt cgaaggatct gtcattccac ctagaccatc
      661 gacaacaatc atgacgattt tggaggcagt ttgttttctg atactagata aatactggaa
      721 atcgatcata atttcttaac cttctatctt ctcacgtagt gaagtaactc ctggtagttc
      781 cctaccttcc aacagctcca aggatgctcc tcctcccgta gacacatgtg tcatctgccc
      841 ttgaaggcca aaaaaattta cggcatccac cgttgaccct ccaccaatta tcgataacat
      901 attcccattt gccaatgctt tcgccactga acgagtgccg gccctggctt tttcccattc
      961 aaatattccc ataggcccat tccaaagaac ggtgttactt tttcgaataa tagacgagta
     1021 aaattccgcc gttgccggtc ctatatccac aataatgtcg ccatcctcta aatcatcaac
     1081 tgattttgtg aggaaatcgg aatctttcag caaagtttta acaactacca catcactggg
     1141 caaagttaaa ttcccgtttt tcgaatcatt ttcatttaac agggtctggg cctggctaat
     1201 aaaatcagcc tctattgagg aatttccgac ttctttattt gaggctgcca aaaaagtggc
     1261 caccatccct cccccaatac aaatgtggct tgctattttt gttagacggt cgacgacctt
     1321 aattttatcc gacaccttcg caccccccaa tatagccgta aagggtaatt tgggattttc
     1381 tagagcattt tctaagtaat tgacctcttt gtctaaaagt agccccgcat atgaaggcaa
     1441 gaatctcgtt acacctaaag tagaggcatg ggcacggtgg gccgcaccaa aggcatcgtt
     1501 tatgaaaact tcggctaatt ttgatagctg ctctgcaaag ccaaggttat tttcttcttc
     1561 ctctctatga aaccgcaaat tttctaatag taggatttcg ccttcactta acttccaggc
     1621 agttgctgct gcttcttctg atacgcaatc ttcaacgaac tgtactttct gttctaggat
     1681 ttcacttaat ctttttgcta ttggctttag agacaattca gggtatcgct tacctttggg
     1741 acgtcctaga tgggagcata ataccaagga acaacctttt cctaataagt gtcttaaagt
     1801 tggtatggaa gccttaatac gggtgtcgtc cgatatgata ctagttcctt ccaagaaagg
     1861 aacattgtag tcaactctca ctaaagccct ttgaccagac agttcaacat cttcaatgct
     1921 tagtttagga ctcatttttc attcctcgat aatttcttct aaataatcga ttaaattctc
     1981 ctcattatca ttcggtaaat tcttctgtac gatggataaa gcatcgtctt tggcggttgc
     2041 atatcggagc tctgctggtt cttttcctcc tagatcacta aggacatcaa gcactgcatc
     2101 aagatcagaa tccgccagaa ctcgtttgaa atacacgtcg cctaattttc ttttctgaga
     2161 aggggtagct ttttccagag caacaatcac aggaaacaat tttttcttgt ttaaaaaatc
     2221 tattttttgg ttggaatcag agccccatat ttg
//
LOCUS       DASCNS010000034         3054 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000317128, whole genome shotgun sequence.
ACCESSION   DASCNS010000034 DASCNS010000000
VERSION     DASCNS010000034.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000317128"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="QF838_01250"
     CDS             <1..992
                     /locus_tag="QF838_01250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59352.1"
                     /translation="NESVELAVHTENLGYSRYWVAEHHNSNSFSGTSPEILIGQIAAK
                     TSTIRVGSGGVMLSHYSALKVAEQFSVLDSFHPNRIDLGIGRAPGSDRRTAAALTYPR
                     PTMDVFSDFPQMVKDLLSFLEDEVEDEHPFAGIRAQSSGKDTYPSVWLLGSSDYSARL
                     AAEMGLPFSFADFFGNTSEYGPQVTDLYRNNFKPSAYLAEPSVNVSLQVICAETEEKA
                     KFVGSSRSINKIISTMGITTKGLLPPEEASVWPLDDQVKAYMEQTTQSYIEGDPNQVK
                     EGILSSSERYQTSDIGIVSNCYYFKDRKKSYSLVAESMGVHPNAKTNIATAEF"
     gene            complement(989..2311)
                     /locus_tag="QF838_01255"
     CDS             complement(989..2311)
                     /locus_tag="QF838_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59353.1"
                     /translation="MFQSAKNKLADSRKNTFYGWYLVPVFGAVMMVATTPLFHAMGIW
                     AVAMEKAFGWNRTQLSLALSFTRVEGGILGPIEGYLVDRFGTKRMVLIGMIIMGFGWI
                     VFSRINHLLIFYFAYLVIALGQGLGSWLALNTMMNNWFVRRRSFAMGLSNSLSRFGAL
                     FFVPLLAWLVDPEFLDRPGWSLTAFILGIVTILLALPLTSLLRNKPEDYGLLPDGDTE
                     EDVERRNKEAAITGVKIEQEYDFTVSQALRSQAFWFISFGHGFTSMVLLAFMLHLAPM
                     MVDQGYTLQTAAFVVSAYTAVSMVFQLIGGYVGDRMPKNVALMMFTLCQALGVFILTF
                     GPPNLFSAYGFALLFGIGFGGRNPISSSIRGDYFGRKNYGKIMGISQVPMNVLLLIGP
                     VFAGLMRDSTGTYQTAFAVLGILAVIGGFCFLFAKKPPHPAAYITNSD"
     gene            complement(2485..>3054)
                     /locus_tag="QF838_01260"
     CDS             complement(2485..>3054)
                     /locus_tag="QF838_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59354.1"
                     /translation="YLSLSSVFRFFILCSSTAPNVVLHPVIFLLPLFTTDVLHRDVDY
                     GGYMMAVNGAGGLLMVFVFSAFGFPKWRGMLCVLSALAGAIMTLLLSQSLWIAPAFLV
                     LAIFGATQTAFRTTNGVLTQTLAEDQYRVRAMSAYRIGMGMVVFFSLFVGWMAGITSV
                     RLTLVFMGALGVVISIAFWIFSPAVRNQE"
BASE COUNT         1022 a          700 c          598 g          734 t
ORIGIN      
        1 taaatgagtc agttgaactg gcagtacata cagaaaacct cgggtattcc agatactggg
       61 tagctgaaca ccacaattcc aactcttttt ctgggactag tccagaaatc ctaatagggc
      121 agatagcagc taaaacctct actattcgtg taggaagtgg cggtgttatg ttgagtcatt
      181 actctgcact taaagtggcc gaacaattca gtgtattaga ttcttttcat cctaatagaa
      241 ttgacctcgg aatcggcagg gcccctggta gtgatagaag gactgccgct gctttaacat
      301 acccaagacc cacaatggac gtattcagtg actttcccca aatggtgaaa gacttgttaa
      361 gttttctaga agatgaagtg gaagatgagc atccattcgc tgggattagg gctcaatctt
      421 caggcaaaga cacatatccg tccgtgtggc ttttgggatc aagtgattat agtgctaggt
      481 tagcagcgga aatgggcctt ccttttagct tcgcagattt tttcggaaat acctcagaat
      541 atggccctca agttactgat ttatacagaa acaacttcaa accatctgcc tatttagcag
      601 aacccagcgt taatgtgagc ctgcaagtga tatgtgccga gacagaggag aaagctaaat
      661 ttgtaggatc ttcgaggagc ataaacaaaa ttatatctac aatgggaatc acaacgaaag
      721 gcttgttgcc tccggaggaa gcgtccgtat ggccattaga tgatcaggtc aaagcctata
      781 tggaacaaac tacccaaagc tacatagaag gtgatcccaa ccaagtaaaa gaaggaatcc
      841 tctcatcatc agaacgctat cagacctctg atatagggat agtatcgaat tgctactact
      901 ttaaagacag aaaaaaatcc tactctttag ttgcagaatc tatgggtgtt catcccaatg
      961 caaaaaccaa tatagctacc gcagaatttt aatctgaatt ggttatatag gcagcaggat
     1021 gaggtggctt ttttgcaaac aggaagcaaa atcctccgat gactgccaat ataccaagta
     1081 cagcaaacgc tgtttgatag gtgcctgtgg agtcacgcat taaacccgca aaaactggtc
     1141 caatcagcaa caacacgttc atgggcacct gagatattcc cattattttg ccataatttt
     1201 ttctgccaaa ataatctcct cgtatggaag aggatatagg attcctacca ccaaatccta
     1261 tcccaaacag taaagcaaac ccgtaggctg aaaaaagatt gggaggtccg aaagttaaga
     1321 taaaaacccc aagagcctga catagggtaa acatcatcaa tgccacattt ttcggcatcc
     1381 gatcccccac ataccctcca atcaattgga ataccataga aaccgctgtg tatgctgaaa
     1441 caacaaatgc agccgtctgc aatgtgtatc cttgatctac catcataggc gcaaggtgca
     1501 gcataaatgc caacaaaacc atcgatgtga atccatggcc aaagcttata aaccaaaagg
     1561 cctgggacct caaagcctgt gacaccgtga aatcatactc ttgctctatt tttacaccag
     1621 tgatcgcagc ttctttgttc cgtctttcaa catcttcttc cgtatctccg tccggaagca
     1681 atccataatc ctccggttta tttctaagaa gactggtcag cggaagggcc agcaatatgg
     1741 tgactatacc gaggatgaac gcagttaaac tccaacccgg cctatctaaa aattctgggt
     1801 ctacaagcca ggctaatagt ggcacaaaaa ataatgcccc aaacctagaa agagaattag
     1861 acagacccat cgcaaaagag cgccttctaa cgaaccaatt gttcatcata gtatttaaag
     1921 ccaaccagct gcctagacct tggccaagag ctataaccaa ataggcaaaa tagaaaatta
     1981 acaaatgatt tatacggcta aacactatcc atccaaaccc catgattatc atcccgatta
     2041 gaaccatgcg tttagtacca aacctatcca ctagataacc ttcgatgggg cctaaaatac
     2101 caccttcgac cctcgtaaat gacaatgcta gagacagttg ggtcctattc cacccaaagg
     2161 ccttttccat tgcaaccgcc cagataccca tagcatggaa gagaggagta gtagctacca
     2221 tcattacagc accaaaaact ggaaccagat accaaccata gaatgtgttc tttcgactgt
     2281 cggctagttt atttttggca ctttgaaaca aagtctaggt actttccaat tacagaatgt
     2341 ttttgatggg aatattagtc taccgcctgc acatgtcatt agatacagga attaagattc
     2401 gcaaatatta actcacaaat actaactggt aaagttttac cccagaggat agtactgaat
     2461 ttgggaggga atttaagcta gaattcactc ctgattccgg actgccggag agaagatcca
     2521 aaaagctatt gatatgacaa ccccaagagc ccccataaac actaaagtta gtcgaacaga
     2581 agtgatacct gccatccagc cgacgaaaag actaaaaaaa acgaccatcc ccataccaat
     2641 tcgataagca ctcatcgccc ggaccctata ttgatcttct gccaatgttt gagttaaaac
     2701 accatttgtg gttcgaaatg cggtttgagt agcaccaaaa attgcgagca ccaaaaaagc
     2761 tggagctatc cacaaagatt ggcttagtaa aagagtcatg atagcgccag caagagcaga
     2821 aagaacacaa agcatgcctc gccattttgg gaagccaaat gcagaaaaca caaaaaccat
     2881 tagcaaacca ccagctccgt taactgccat catataaccg ccataatcaa catctctatg
     2941 aaggacatct gtggtgaaca aaggcaacaa aaatatgaca ggatgcaata caacatttgg
     3001 tgcggttgag gagcataaaa tgaaaaaacg aaaaaccgat gataacgata agta
//
LOCUS       DASCNS010000035         2976 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000323258, whole genome shotgun sequence.
ACCESSION   DASCNS010000035 DASCNS010000000
VERSION     DASCNS010000035.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000323258"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="QF838_01265"
     CDS             <1..1255
                     /locus_tag="QF838_01265"
                     /inference="COORDINATES: protein motif:HMM:NF021351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HJO59355.1"
                     /translation="LSENDRSVHAYAFFVSSVSSDNQRQSAEDLLNEFDRRSHNFSFS
                     FVDPELNRSEALKYNVTTYPSVVFEADDGKFEGVTLLTEQDFVTGILISTGTEQKAIY
                     YLTGHGETSVSRDPMTGAIGLEGLDLAIEGMQRDNYFVMPLNLKQFESVPDDAAVLII
                     AGPKDNKDLDESELTAILKYVRDGGRILMLLDPNPPVKFNGLAALYGIAISSEHVVDA
                     VSNVSGKMLTPMVQKANGQFTTSNSAPGLTIADDISVTIFPDSAAILSPSAEEFALRD
                     HIKFTPLGMTTPASWLTKDPEDVTYSSEKQQGPFPISAVVEATGMYTDLGATHQLAKI
                     VLFSDSDFASNKYFASMDNADIFLNSINWLADDFELISIRPKLLPYRELVVNSRERDF
                     IKWSSWTLPPTFMLFMALVVWWRRR"
     gene            1268..2287
                     /locus_tag="QF838_01270"
     CDS             1268..2287
                     /locus_tag="QF838_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59356.1"
                     /translation="MNLKATVALIILAAVAVVVYLFNPFAQEDKKPPPKPWFYQVSVD
                     DMTSIRITHEDKSESFIKTPSGTWAFDWDVLIPPNHNRWGGIAFILGGPQTKRDLTET
                     TTLIVDPSQYGLDDPHTVVNIGLTLNRTLEFRLGDKTTNGWHHYGQIVGFPQLFLIAD
                     TWGEVLARLAVEPPYPKWYIERPADQISEINIFPKSEDAKTDKAQLNFKREKDGTWRV
                     IDYLVGTESHLVDDTKWQKLLPTIKSPSGVDVAVHKVRDRDYSQWGLGDNSAAVEIRF
                     QNTTEQGTTYTDGSLLLIGDLEPGGQAYYGKSEEEGVTQPVLLFPKLWVETIFGLYEN
                     IPYAD"
     gene            2332..>2976
                     /locus_tag="QF838_01275"
     CDS             2332..>2976
                     /locus_tag="QF838_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HJO59357.1"
                     /translation="MDAEIIAVGTELLMGELVDSNSAFLASELPKLGMSLKIAVKIGD
                     DINDLLKGIESALIRSDVVITTGGLGPTSDDLTRESIAKFFEEEMEVDPTLLNDLEDN
                     FALRGIKMPNPNNKQATLIPSSIPLPNPNGTAPGWFVNKDGKIVIAMPGPPMELIPMW
                     KNHVVEKLAALSGGISIATKNIKTFGRSEGELDEILSHLFGQENPYLGIYSKQDG"
BASE COUNT          934 a          649 c          629 g          764 t
ORIGIN      
        1 cctttccgaa aacgatcgat cagtgcatgc atatgctttt tttgtatctt cagtatcctc
       61 cgacaaccaa cgacaatcag ctgaagacct attaaacgaa tttgacagga gatcccacaa
      121 ttttagtttt tcatttgtgg atccagaact aaacagatca gaagcattga aatataacgt
      181 gacaacttac ccatcagttg tctttgaggc agatgatggc aaatttgaag gcgttaccct
      241 cttaacggaa caagatttcg ttactggtat attaatttca acaggaacag agcaaaaagc
      301 catttactac ctaactgggc atggtgagac atcggtatct agagacccca tgaccggtgc
      361 aattggattg gaaggactag atctagcaat agaaggcatg caaagggaca attattttgt
      421 gatgcctttg aatttgaaac agtttgaatc cgtgcctgac gatgctgcag tattaataat
      481 tgcaggcccc aaagacaata aagacttgga cgagtcagag ttaacggcaa ttctaaaata
      541 tgtccgagac ggcggaagaa tattaatgct actcgatccg aatccaccag taaaatttaa
      601 cggtctagcc gccctttacg gtatagcgat atccagtgaa catgtggtag atgcagtaag
      661 caatgtctca gggaaaatgc tcacccctat ggtgcaaaag gccaatgggc aatttaccac
      721 aagtaactcg gcccctggcc ttacaatagc tgatgacata tcagtaacaa ttttcccaga
      781 ttcagctgcg atactcagcc cttcagctga ggaatttgca ctaagggacc atatcaaatt
      841 cactccactt ggtatgacta ctccagcaag ttggctaacg aaggacccag aagatgttac
      901 atattcttca gagaaacaac agggcccttt tcctatatcg gctgtagttg aggccacagg
      961 aatgtatact gacttaggag ccacccacca actagctaaa atcgttctat tctcggactc
     1021 tgattttgcc agcaataaat acttcgccag catggataat gcagatattt tcctcaattc
     1081 aattaactgg cttgccgatg attttgagtt gatatccata aggcccaaac tactgcccta
     1141 cagggaattg gttgtgaaca gtagagaaag ggatttcatt aagtggtcca gttggacact
     1201 acctccaacc ttcatgctct tcatggcttt ggttgtatgg tggagacgta gataagagag
     1261 atcaaaattg aatcttaaag ccaccgtagc attaataata ctggcagctg ttgctgttgt
     1321 cgtttaccta tttaatcctt ttgcccaaga ggataagaaa cctcctccaa aaccttggtt
     1381 ttaccaagtt tcggtagatg atatgacatc aatccgaatc actcacgagg acaagagtga
     1441 atcatttatc aaaactcctt caggtacctg ggcatttgat tgggatgtat tgatcccccc
     1501 taatcacaat agatggggtg gcattgcttt tatcctagga ggaccgcaaa cgaaacgaga
     1561 tctaaccgaa acaactaccc taatagttga cccgtcccaa tacggcctcg acgatccaca
     1621 cacagtagtt aatatcggcc tcactcttaa taggacattg gaatttagac ttggagacaa
     1681 aaccaccaac ggctggcacc attacggtca gatcgtggga ttccctcaat tgtttcttat
     1741 agccgacacg tggggtgaag tgctagctcg attagcagta gaaccaccct accccaaatg
     1801 gtatatagaa cggcctgcag atcaaatcag cgaaataaat atttttccta aatcggaaga
     1861 tgctaaaacc gacaaggctc agctgaactt caaaagagaa aaagatggga cttggcgagt
     1921 gatagactat ctcgtcggca cggaatccca cttggtcgat gacaccaaat ggcagaaact
     1981 tttacccaca ataaaaagcc cttctggagt tgatgttgcg gtccacaaag ttcgggaccg
     2041 agattattcc caatggggcc taggagacaa ctcagcagca gtagaaatac gtttccaaaa
     2101 cacgacggaa caagggacaa catacaccga cggctcttta ctgctaattg gtgaccttga
     2161 acccggcgga caagcctatt acggaaagtc tgaagaagaa ggagtgactc aaccagtact
     2221 acttttcccc aaactgtggg tagaaaccat atttgggtta tacgaaaata ttccatacgc
     2281 tgattaaatt tgactgagtc gacctcttag aattcgggat ggcttttggt tatggatgct
     2341 gaaattattg cggtcgggac agaactactt atgggagagt tggttgattc aaattcggct
     2401 ttcctagcat ctgaactacc taaattggga atgtcattaa agattgctgt caaaatcgga
     2461 gatgatatta atgaccttct aaaaggcata gagtccgcat taattagatc tgatgtggta
     2521 ataaccactg gcggactcgg accaacatct gacgacctga caagagaatc aatcgccaaa
     2581 ttttttgagg aagaaatgga agtggacccg acacttctca atgatttaga agataatttt
     2641 gcgctgcgcg gcataaaaat gccaaacccc aacaacaaac aagctacttt aataccttca
     2701 tccattccgc tacctaatcc gaatggaacc gccccgggtt ggtttgtaaa taaagacggg
     2761 aaaatagtaa tagcaatgcc cgggcctcca atggaattga ttcctatgtg gaaaaaccat
     2821 gtggtagaaa aacttgcagc actttcaggc ggcatttcca ttgcaacaaa aaatatcaaa
     2881 acattcggcc gatctgaagg agaattagat gaaatactgt cacatctttt cgggcaagaa
     2941 aatccttatc tcggtattta ttctaaacaa gatggc
//
LOCUS       DASCNS010000036        14340 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000323281, whole genome shotgun sequence.
ACCESSION   DASCNS010000036 DASCNS010000000
VERSION     DASCNS010000036.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 14340)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14340)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14340
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000323281"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="ccmA"
                     /locus_tag="QF838_01280"
     CDS             complement(<1..627)
                     /gene="ccmA"
                     /locus_tag="QF838_01280"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HJO59358.1"
                     /translation="MDVLLKVENVTKSYGRNRVLHRLCLSVEPSEVVVIRGINGSGKT
                     TLLSIIASLVSCDYGRVKILGQDLRSNAHRVRSDLAFVAHQPFLYSQLTVKENLEFFG
                     RLNSIDSLDSTILEKAALIGLYDRLDSKIATLSHGYIKRVAIARALLHNPRILLFDEP
                     ESGLDQDSLMLFERIIGDQLEAGKSIVMTTHATQISYRGNVRNQRISKG"
     gene            734..1153
                     /gene="queF"
                     /locus_tag="QF838_01285"
     CDS             734..1153
                     /gene="queF"
                     /locus_tag="QF838_01285"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HJO59359.1"
                     /translation="MPSIEELQTAYESLDDKIEIVSEDSINPELLIAFDYEYSGSDTK
                     VFITTEEFTAVCPWTGLPDFGTLTIEYVPSNLCLELKSLKYYLMGFTGVGIVQEHAAN
                     KIIKDLIKCCKPEEISIELEYKVRGGITTKVSVTHKK"
     gene            1261..2028
                     /locus_tag="QF838_01290"
     CDS             1261..2028
                     /locus_tag="QF838_01290"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HJO59360.1"
                     /translation="MTNYVRFQNNENSSFGVLEGETISEISNAPFSSYETTGNTFSLG
                     NVKLLSPVAPNKIVAIGLNYKSHLGDRPGPEAPEPFLKASTTVVGHGDDIVIPKIAIA
                     EGAKVQPEAELALVIGKECKGATQANALDYVFAYTCGNDVSVRDWQQNDLQWARAKSS
                     DTFGPIGPWMTTDLDPTNIQVICRVNGEEKQNQNTSDLLHPVTKIIEYVSAVITLEVG
                     DVIMTGTPGTPGDIHPGDTVEVEISGVGVLSNPVVSE"
     gene            complement(2085..2807)
                     /locus_tag="QF838_01295"
     CDS             complement(2085..2807)
                     /locus_tag="QF838_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59361.1"
                     /translation="MDVSILFAFTAGTVAAFNPCGAAMFPAYVGFHLSKSSSSLSPLF
                     ILLKGLFIGLTLTIGFVVVFGVFGIFMALGFRLFGSLLPFIGLGIGTLIFLTGLWLLL
                     MNRSLPVSFLGNLSFGSFQGINQIFMFGIAYALASLSCALPVFLSAVGIVVGSGVSVA
                     GVTNVVAGSLAYSLGMGLIMMSVTVAVLYFEDATQRLVGILIPYVELIGKLSMVAAGA
                     YIIVYWLIGDGAELLRYRLGRT"
     gene            complement(2846..3208)
                     /locus_tag="QF838_01300"
     CDS             complement(2846..3208)
                     /locus_tag="QF838_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59362.1"
                     /translation="MSDRYKNEIEKILEDTPELPDAPLEAIGPQESFYDQILSLFHMS
                     RDRKFGSVSAFNMFLLSLIFFAGFFIIKMNLFAVFGISSLIVSYLTFVWPTSLNRPGL
                     FLNRFMTWSGGLFKKKDK"
     gene            3413..4789
                     /gene="tig"
                     /locus_tag="QF838_01305"
     CDS             3413..4789
                     /gene="tig"
                     /locus_tag="QF838_01305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HJO59363.1"
                     /translation="MKITQDDVVDRQTVLHIELEDDDIDPYLDRAYQRVVQRVNIPGF
                     RKGKAPRRIIEQYFGRESMLNEVLDSMLPELTNQAINEKDLDAVGLPSIEMEELDPFQ
                     FSAIVPLRPEVDLGEYSEIRIEKEHPKIEDDAVDSRIEQLRLSVATWEPTERPIEIGD
                     MITAQIKGTVGEKTIFDESDAVYLVNEEIGRPFPGFSEKLVGMEPDKPSQFDLSIPED
                     FADPDLANQDASFDVTIKDIKARVLPEIDDEFAKSIGEGHETLGDLKEEVQKSIQTEA
                     EEEITRTHRESIVEALMDTAKVEMSALLLQHEAEHMVEEQERMVSQANMNMDDYLASL
                     GKTREEFVEESKTEASDRLKRSFVLAKLAEEEGLEVSDEDIDDRITEMFSNAEQEIPE
                     SSQNAQMRDYLSRSLLMEETMKKLEGIAAGESEQELANQEPDVEDVDDGSSNDNKDQE
                     TEEGEVNA"
     gene            4782..5393
                     /gene="clpP"
                     /locus_tag="QF838_01310"
     CDS             4782..5393
                     /gene="clpP"
                     /locus_tag="QF838_01310"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HJO59364.1"
                     /translation="MLDRPENIIPYVVERDPRGERTYDIYSLLLKERIVFLGTGINDQ
                     VANAIIAQLLFLDREDPERDINLYINSPGGVITAGLAIYDTMQLIRPDVSTICVGMAA
                     SMGTVLLCGGADGKRYALPNSTIHMHQALGGAQGQASDIEIAAREIIRMQDKIRNIIS
                     EHTGQSYEKIARDTDRDFYMSSEQAEEYGLIDVVMSSPNNSAE"
     gene            5402..6694
                     /gene="clpX"
                     /locus_tag="QF838_01315"
     CDS             5402..6694
                     /gene="clpX"
                     /locus_tag="QF838_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HJO59365.1"
                     /translation="MSTTQNDYLCSFCSKPQAQVKRLIAGPDRVFICDECVELCQQII
                     SEETPEDIEDLDNPVEKGITPKFIYDQLEEYVIGQEKAKKILSVAVYNHYKRVWSNKH
                     SDEKIELQKANILMYGPSGSGKTHLAQTLAKILDVPFAIVDATSITEAGYVGEDVENI
                     LLRLIQAADYDISKAEHGIIYIDEIDKITRKSPNPSITRDVSGEGVQQALLKIIEGCL
                     ANVPPQGGRKHPQQEFMQIKTDDILFMVGGAFEGMAELLMKRVHKDNYSVGFKQASNH
                     NEGSQSVIDSVRHQLNPDDLMEFGFITEFVGRLPVLVTLDELTKDDLVRILTEPKNAF
                     VEQYKALFRMENVNLNFSKDSLLATAEQAIEQKTGARGLRTILESTLMEVMFELPSMS
                     GITHCDVEKETITGDSPVKLSNDSSEIIELATLEKKSA"
     gene            complement(6708..8516)
                     /gene="aspS"
                     /locus_tag="QF838_01320"
     CDS             complement(6708..8516)
                     /gene="aspS"
                     /locus_tag="QF838_01320"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HJO59366.1"
                     /translation="MLKDISCGALRSQDIGKSVTLAGWVHRRRDHGNLIFIDLRDRDG
                     LVQVVFNPELAEKAHVIAQLLRNEWVIQVSGEVLARPEGTVNEDMLSGAVEVYASELT
                     VLNQSLTPPFYVNEDTTVDENVRLKYRYVDLRREAMKNAMIIRHKVVKYIRDFLDSEG
                     FLEIETPILIKSTPEGARDHIVPSRLYPGNFYALPQSPQQLKQLLMVAGFEKYFQIAR
                     CFRDEDSRADRQPEFTQLDLEMSFVTQEDVLSLTEDLFTGLLESLFPDKNFMKPFPKL
                     SYEQSMEDYGTDKPDLRFEMKMSDLGNTAKKTEFNVFHNVLDNGGIVKGFSAPGCGEF
                     TRGQIDELTEFVKERGASGLIAIGINGAGESLEPIDMDKVRSNITRFLTPENVKDFAI
                     STGAANGDLVLLVAGEPKSTNASLAALRHEMGIRLELADPNMMMFAFVTDFPLFEWNL
                     EERRWDASHHAFCMPKDGFSKYLSNDPGKVIAQSYDLVCNGLEMASGSIRVHNRELQE
                     DIFGVLGYSREEVSERFSQLLDAFEYGAPPHGGIAPGIDRLIMILMGKDSIRDVIAFP
                     KTQSQMDPLFGAPSAVEQSQLEELHIEVVGLEVEEG"
     gene            complement(8547..8987)
                     /locus_tag="QF838_01325"
     CDS             complement(8547..8987)
                     /locus_tag="QF838_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013224.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJO59367.1"
                     /translation="MVDDLDNQIIETLSLDGRMSNASIARNLGVSEGTIRRRLNILKD
                     EGIIDIKVVLNPNYVASETEAIIGIQVDLPVIRQVVLKLNQINEIRWVNITSGSFDIF
                     VNVSTKSLSDLLELLQNKIGNIDGVKKIETFTTLDVPKDKLRGI"
     gene            complement(9080..9757)
                     /locus_tag="QF838_01330"
     CDS             complement(9080..9757)
                     /locus_tag="QF838_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014690.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HJO59368.1"
                     /translation="MNFSQYIVFIAVPCCCYLIGAIPISYLFGRFWAKIDIRTQGSRN
                     AGASNLAVYGGRNIGIIAGLLDCLIKGTFTLLFLDVILGLGPYFLLIASMSLIAGHNW
                     SIFIGLEGGRGISTAFGLLIGFQMWEEILVLTIFLGIIGRLILYKDSGFWCFISFGLL
                     PLLCFAFQEQTHIIIFSVLLVIMLLSKRLMSNGNMIREGSLKSTLLCRLVFDRDILSK
                     TSWLKRG"
     gene            complement(9747..10610)
                     /locus_tag="QF838_01335"
     CDS             complement(9747..10610)
                     /locus_tag="QF838_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014826.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HJO59369.1"
                     /translation="MDHIKVDLHLHTNHSDGDLSPSDLVELCFERGLQTIAVTDHDTT
                     SALQEARECASKFGIRLINGIELGTNYLDDEVHILGYGIDLQDAGFQDKLSIFRKDRL
                     LRGKKIIEKLVSIGLNISWEMVRNIAKGDSVGRPHIAMALVELGYAANIKDAFKKYLN
                     TDSPGFIPREMVRPTEAVEILRDNGALPVLAHPLLSNSKSGRKSIQNLVELIPELCEA
                     GLMGIEVYYGDYDSQQVSRLIELAEKYSLIKCGGSDYHNSENRDDPHPGDVGPPQESV
                     KQIDLAISREF"
     gene            complement(10686..12212)
                     /gene="rny"
                     /locus_tag="QF838_01340"
     CDS             complement(10686..12212)
                     /gene="rny"
                     /locus_tag="QF838_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HJO59370.1"
                     /translation="MDIMTIAIAGLISLIVGTVLGSSFKRFQTYRSKVAARSEAEHML
                     EQALEQQRSILLEAKEEALKLQKNGDEELKERRGEVRRIERRVASREENAEKRSQNLE
                     RRERKLNDRENEIEGLYEEAEGVKSQSIQRLETVADMTMDDAKDVIMATAEDDYRHEL
                     ALKYRDMEESTKNEANDKARMILGQAIQRLASDVVSEATVSTVPIPSDDMKGRLIGRE
                     GRNIRSIERNTGVDLIIDDTPEAITLSCFDPVRREVARLAVSKLVADGRIHPARIEDM
                     VKKSQEEVEETIWKSGESAVLEADVRGLHPELIRLLGRLKYRFSYGENVLMHCLEVAH
                     LAGLMAAEVGANVKVAKVGGLLHDIGKALSHEIEGPHAEIGADIAKKYKVSHRVTTCI
                     GEHHDDEMSSVESFIVAAADALSAARPGSRKDTVENYVKRMEELEEVAGDFAGVEKCF
                     AIQAGREVRIMVEPDSIDDVSATSLARDVVKNIEEKLAYPGQIKVVVIREKRSVEYAR
                     "
     gene            complement(12334..13686)
                     /gene="ffh"
                     /locus_tag="QF838_01345"
     CDS             complement(12334..13686)
                     /gene="ffh"
                     /locus_tag="QF838_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HJO59371.1"
                     /translation="MFETLTDRLTSVIKSISNKGHLTEKDIDTTLKEVRRALLEADVN
                     FKVARSFVASIKEQVIGEEILSSITAGQHVVKVTNDELIKTLGGVSVELTKKEKHNTV
                     LMVGLNGSGKTTTSAKLAKHLKDSGQSVGLVAADVHRPAAIEQLSVLADQIGISLYEA
                     GTEVSAAKVSIDGYEAGKNRNFDWTIIDTAGRFQVDSELMDELEEIQKSINPTEVLLV
                     VDAMTGQESVNVAQNFHDRIGLTGLVLTKMDGDARGGAALSITSVTGIPVKYIGVGER
                     IDALEKFHPDRLASRILGMGDVLTLAEKAQATFDQDQMADLERKIKQSTFDLEDFLNQ
                     MQAVKKMGSMSQILEMVPGFSMMKGKVNADDLDDNHLVKAESIIYSMTPDERSKPEMI
                     GGSRRRRIAKGSGTSPQDVNQLLNQFKQVRKMMKEMSSSGGQKKMMRMLSQKGNPFGF
                     "
     gene            13838..>14340
                     /locus_tag="QF838_01350"
     CDS             13838..>14340
                     /locus_tag="QF838_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024874.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO59372.1"
                     /translation="MEINWLGHSSVSINSRDLVLIADPYDSSDGTFMGPQQADIVTIS
                     HDSVKHSNVDSISGNPRVLDGPGEYEINNFYIKGFGTALTTVDQGSPRKINTIYTIKA
                     EGLSLCHIGALSQKFTPSQIDEIGQTDILFVPVTPPEDQDNTLIQDTITALQPRIVLP
                     IQYTLGNP"
BASE COUNT         4482 a         3245 c         2668 g         3945 t
ORIGIN      
        1 tccttttgat atgcgttggt tgcgaacgtt acctctatat gaaatttgag tggcatgcgt
       61 agtcataaca attgacttgc cagcctctaa ttgatcacca ataattcttt caaatagcat
      121 caaagaatct tgatctaaac ctgattccgg ttcatcaaac aataatattc tagggttatg
      181 aagaagtgcc ctcgcgattg ccactctttt gatgtaacca tgcgacaagg tcgcaatctt
      241 gctatctagt cggtcgtaga gtccgatcag tgctgctttc tctaaaatcg tactatccaa
      301 gctatctatc gaattcaagc gaccaaaaaa ttctaaattt tcctttactg taagttgcga
      361 atatagaaaa ggctgatgtg cgacaaacgc caagtcagat ctaaccctat gagcatttga
      421 ccttaggtct tgcccaagta ttttaaccct accgtaatcg caggagacca aagaggctat
      481 gatcgacagc aatgtagttt tcccagaacc attgataccc ctaataacca ctacttcact
      541 gggttctaca gaaagacaca gtctatgaag aacccggttc ctaccgtacg attttgttac
      601 attttctact tttaaaagta cgtccacgca atttctcgca taacagagta tttatataga
      661 atataccttt aatatggtat ttcgttgtta caattaacag agtaacagag gaatacgata
      721 atggagagta attatgccct cgatagaaga attgcaaacc gcgtacgagt cactcgacga
      781 caaaattgaa atagtttccg aagattcgat aaatcctgag ttgttgatag cgtttgatta
      841 cgaatattcc ggatctgata ccaaagtatt cataacaaca gaagaattca ccgcagtctg
      901 tccctggact ggtcttcccg actttggaac actcaccatt gaatacgtac cgtctaattt
      961 atgcctcgaa ctcaagtcgc tgaagtacta tttaatgggg tttaccgggg ttggaattgt
     1021 ccaagaacac gctgcgaaca aaataatcaa ggatcttata aaatgctgta agcctgaaga
     1081 gatcagtata gaactggaat ataaggtcag gggaggcata accactaaag tttcggtaac
     1141 ccacaaaaaa taaagtcagt tccttggaac tactttaacc tatcagttaa actgaccccc
     1201 atccttttgg attatgatca atataaagtt cataaacaac ctcaatctag ggagagaaat
     1261 atgacaaatt atgtaagatt tcaaaataac gagaattctt ctttcggagt tttagaaggg
     1321 gaaacgatat ccgaaatatc caacgcacct ttcagttctt acgaaacaac aggtaacacc
     1381 ttctctttgg ggaacgtaaa attattatca cctgtagccc ctaacaaaat agttgccatt
     1441 gggctcaact ataaaagtca tcttggagac cggccaggtc ctgaagctcc agaaccattt
     1501 ctcaaggcat ccacaactgt ggtagggcac ggggatgaca ttgtaatacc caaaatcgct
     1561 atcgctgaag gtgccaaagt acaacctgag gcagaattag cattagtaat cggaaaagaa
     1621 tgtaaagggg caactcaggc caacgcgctt gactacgtct ttgcctacac ttgcggcaat
     1681 gatgtttcag ttcgagactg gcaacaaaac gatttgcaat gggcacgggc taaatcttca
     1741 gatacctttg gtccgattgg gccttggatg acaacagacc tcgatccaac aaacatacag
     1801 gttatatgca gggtaaacgg agaagaaaag cagaatcaaa acacatctga tttactacac
     1861 ccagttacca aaatcattga atatgtcagc gcagtcataa ccttagaagt aggggatgtg
     1921 ataatgactg gtactcctgg tactcctgga gacattcatc cgggagacac tgtagaggta
     1981 gaaattagcg gtgtaggagt gctaagcaac ccagtagttt cagaataatc aacataatta
     2041 tggttgatag tacgggatgg cattccggct ccatcccgtc atcttcaagt tcttcccaac
     2101 cgatacctca ataattcagc accatcacca atcagccaat acacgataat ataggcccct
     2161 gctgctacca tcgacaattt acctatcagc tcgacatagg gaattaatat gcctaccaac
     2221 cgttgagtag catcctcaaa atataagacc gctaccgtga cggacatcat tatcaaaccc
     2281 ataccaaggc tataggcaag cgacccggcc acaacatttg ttaccccagc gactgatact
     2341 ccagacccaa ctacgattcc tacagcagat agaaaaacag gcaaagcaca actcaaagag
     2401 gcaagagcgt aagcgatgcc gaacataaat atttggttaa ttccttgaaa ggacccaaag
     2461 gataaattcc ccaaaaaact gacaggcaag ctacgattca tcagaagcaa ccaaagaccg
     2521 gtaaggaaaa ttaacgtccc aatacccaga cctataaaag gcagtagtga cccgaaaagc
     2581 ctaaacccta atgccataaa aattccaaaa acaccaaata ccacaacaaa cccaatggtg
     2641 agcgtaaggc caatgaataa accttttaac aaaataaata agggggatag ggaagaagaa
     2701 gatttactta aatgaaaccc aacataggcc ggaaacatgg cggcgccgca tgggttaaat
     2761 gctgctactg tcccggctgt gaaggcgaat aggatcgata catccataat tcaggtgata
     2821 ccttttatgc tttttgcaaa cccacctatt tatctttctt cttgaacagt cctccactcc
     2881 aagtcatgaa cctgttcaaa aacaaaccag gcctgttaag ggaagttggc caaacaaatg
     2941 ttaggtaaga cacgatgagc gaactaatac caaaaacagc aaataagttc atcttgataa
     3001 taaaaaagcc ggcaaaaaaa atcaaagata aaagaaacat gttaaacgct gaaaccgagc
     3061 caaatttacg gtccctagac atatgaaaca aagaaagtat ctggtcgtaa aatgattcct
     3121 gaggcccaat ggcctccaag ggggcgtctg gaagctctgg ggtatcttcc agtatttttt
     3181 ctatctcatt cttgtatcta tctgacatag gttcaaatag ctccaaaaac taatctcacc
     3241 aaatattaac aacacgtatg aatcgtaatt taggacacga attcctgtca acgataaatg
     3301 tcccaaacat acacatataa aggaggtttt tactggctaa atgccaatcg agtttatcgt
     3361 ggtgatataa ttatcttcct gcttttttaa aaatcactcc taggagacac tggtgaaaat
     3421 aactcaggac gatgtcgtcg acagacaaac agtgcttcac atcgaacttg aagatgatga
     3481 tatcgaccca tatttggata gggcttatca acgggtggtt caacgagtca atatacctgg
     3541 tttcaggaag ggtaaagctc cacgaagaat cattgaacaa tattttggta gagaatcgat
     3601 gctgaatgaa gttctggatt ccatgcttcc agagcttaca aaccaagcaa tcaatgagaa
     3661 agatttggat gctgttggcc tccccagcat agaaatggag gaattagacc cattccaatt
     3721 ctcagcaatt gtgccactta gaccagaagt tgatctgggt gaatactcgg aaatcagaat
     3781 tgaaaaagag caccctaaaa tagaagatga cgctgtagac agccgtattg aacaactccg
     3841 actaagtgtt gccacatggg aacctactga aaggcctatt gagattggag acatgatcac
     3901 agcccaaata aagggaactg ttggggaaaa gacaattttt gatgaaagtg atgctgttta
     3961 tttagtaaac gaagaaatag gccgaccttt ccctggattt tccgaaaagc tggtaggaat
     4021 ggaaccagat aaaccatctc aatttgatct ttcaatccca gaagattttg cagatcctga
     4081 tcttgctaat caagatgctt catttgacgt gactatcaaa gatatcaagg caagagtatt
     4141 accagaaatc gatgatgaat tcgccaaaag catcggagaa ggtcacgaaa ctcttggtga
     4201 tttaaaagaa gaagttcaaa aaagcattca aaccgaagct gaagaagaaa ttacaagaac
     4261 tcacagggaa tccatcgtag aagcattgat ggacacagcc aaagtagaga tgtcagccct
     4321 attattgcaa catgaagcag agcatatggt tgaagaacag gaaagaatgg tttctcaagc
     4381 caatatgaat atggatgatt atctagcctc cttagggaaa acaagagaag aattcgtaga
     4441 agaatcaaaa acagaagctt ccgacagact aaaacggtcc tttgtattag ctaaattagc
     4501 tgaagaagaa gggctagagg tttcggacga agacatagat gacagaataa cagagatgtt
     4561 ttctaacgca gaacaagaaa tacctgaatc aagtcaaaac gcgcagatga gagactactt
     4621 gagtagatca cttctcatgg aagaaaccat gaagaagctg gaaggtatcg ctgctggaga
     4681 atctgaacaa gaacttgcta accaagaacc tgatgtggaa gatgtcgatg atggttctag
     4741 caatgataat aaggaccagg aaacagaaga aggagaagta aatgcttgat agacctgaaa
     4801 atataatccc gtatgtagta gaaagagatc caagagggga gagaacctat gatatttact
     4861 ctcttctact aaaggaaagg atcgtttttc taggaactgg aattaacgac caggtagcaa
     4921 atgcaataat tgctcaatta cttttccttg acagagagga tccagaaaga gacataaatc
     4981 tctatatcaa ctcacccgga ggagtaatta cagcaggtct agcaatatac gacactatgc
     5041 aattgatcag acctgacgtc tccacaatat gtgttggcat ggcagcgagt atgggtacag
     5101 tactgctgtg cggaggggct gacggaaaga gatacgccct acccaattcc acaatacaca
     5161 tgcatcaggc attaggcggt gcacagggtc aagcctcaga tatagaaatt gctgcacgcg
     5221 aaatcatcag aatgcaagac aaaattcgaa atatcatctc tgaacacaca ggccaatcat
     5281 acgaaaagat cgctagagat acagaccgag atttctacat gagttcagaa caagccgaag
     5341 agtacggcct cattgatgtg gtgatgtcct ctcccaataa cagcgctgaa tagaggtata
     5401 aatgtccaca actcagaacg attacctatg ttcattttgt agcaaacctc aggcacaagt
     5461 aaaaaggcta atagcaggac ctgacagggt tttcatatgc gacgaatgtg tcgagttatg
     5521 ccaacaaata atttcagagg aaactccgga agacatagag gatctagata acccggtcga
     5581 aaaaggtatt accccgaaat tcatttatga ccaactggaa gagtacgtaa tcggccaaga
     5641 gaaggcaaag aaaattctaa gcgtggctgt atacaaccac tacaagaggg tttggtcgaa
     5701 taaacattca gatgaaaaaa tagagcttca gaaggcaaat attttaatgt atgggccatc
     5761 tgggtcaggc aaaactcacc ttgcacaaac tctggcaaaa atactagacg taccatttgc
     5821 catcgtagac gcgacttcaa taacagaagc gggttatgtt ggagaagacg ttgaaaatat
     5881 acttttgaga cttatccaag ctgccgacta cgatatttca aaagcagaac acggaatcat
     5941 atacattgat gaaatagata agatcacgag aaaaagtcca aacccttcaa ttactaggga
     6001 tgtatccggc gaaggtgttc agcaggctct tctaaaaatc atagagggat gtcttgctaa
     6061 tgtccctcca caaggtggtc gaaaacaccc tcaacaagaa ttcatgcaaa taaaaactga
     6121 tgacattctc tttatggtag gtggtgcatt tgaaggcatg gctgaactac taatgaaaag
     6181 agtccataaa gataactaca gcgtgggatt caaacaagcc tccaatcata atgagggcag
     6241 ccaaagtgtg atcgattcag ttcgtcacca acttaatcct gatgacttaa tggaatttgg
     6301 tttcatcacc gaattcgtag gtagactacc ggttctagtg actttagatg aactcacaaa
     6361 agacgatttg gtacgaatat tgactgaacc caaaaatgca tttgtagaac aatacaaagc
     6421 cttgttcaga atggaaaatg ttaatttgaa ttttagtaaa gattcattgt tagccacagc
     6481 tgaacaagcc atagaacaaa agaccggagc cagagggtta cgcactattt tggaatcaac
     6541 tctcatggaa gttatgtttg aactacccag catgagtggc ataacacact gtgatgttga
     6601 gaaagagacc attacagggg actctccagt gaaattgtca aacgacagca gcgagataat
     6661 cgaacttgca accctggaaa agaaatctgc ttagctaata tcgcttctta gccttcttca
     6721 acctcaaggc ccactacttc aatatgaagc tcttctaatt ggctttgttc tacagctgaa
     6781 ggagctccaa acaatggatc catctggctt tgcgttttag ggaacgcaat tacatctctt
     6841 atcgaatctt ttcccataag tatcataatg agcctatcta tgcctggtgc gattcccccg
     6901 tgtggaggtg ctccatattc gaaggcatcc aacaattggg aaaacctttc cgatacctct
     6961 tctctagaat agccaagcac tccaaatata tcttcctgca attccctgtt gtgaaccctt
     7021 atacttccac tcgccatttc aagcccgtta caaactaagt catatgactg tgcaataacc
     7081 ttacctgggt cattggataa atacttacta aatccgtcct taggcatgca gaaggcatgg
     7141 tgggatgcat cccatctcct ctcctccaaa ttccattcaa atagcggaaa atctgttaca
     7201 aatgcaaaca tcatcatatt tggatcagcc aactctaacc ttatccccat ttcatgacgt
     7261 aatgcagcta aggacgcatt agtcgatttt ggttcccccg ccacaaggag cactaagtct
     7321 ccgtttgcag caccagtaga aatagcaaaa tctttaacat tctctggagt caaaaatcga
     7381 gtaatatttg atctaacttt atccatatca attggttcca atgattcacc agcaccatta
     7441 ataccaatgg ctatgaggcc acttgctcct cgttccttaa caaattctgt taactcatct
     7501 atttggcctc tggtgaattc tccacaacca ggagcagaaa accccttaac aatcccaccg
     7561 ttatccaaaa cattatgaaa aacattgaat tctgttttct tcgctgtatt tcccaaatct
     7621 gacatcttca tttcaaatct cagatctggt ttgtctgtac cgtaatcttc catactttgt
     7681 tcgtaagata gtttaggaaa aggtttcata aagttcttat cgggaaacaa agactctaac
     7741 aatccagtaa acaaatcttc agtcaaagag agaacatctt cctgggttac aaaactcatc
     7801 tccaaatcta gctgtgtgaa ttctggctgt ctatcagctc tagaatcttc gtctctaaaa
     7861 cacctagcta tctgaaaata tttctcaaac ccagctacca tcaaaagttg cttaagctgc
     7921 tgaggagatt ggggcaaagc ataaaagtta ccagggtata accggctagg aactatgtga
     7981 tctctagctc cttcaggcgt gcttttaatt agtatagggg tttcgatttc caaaaaaccc
     8041 tcagagtcta agaaatcgcg tatatatttc acaactttat gcctaattat catggcattt
     8101 ttcatggctt ctcttctaag gtcaacatac cgatatttga gtcttacatt ctcgtcaact
     8161 gtagtatctt cgttaacata aaagggagga gtcaaagatt ggtttaaaac tgtcaattct
     8221 gaagcatata cttcaacagc tcccgacaac atatcttcat ttacagtgcc ttctggccta
     8281 gccaaaacct cacccgatac ctgtatgacc cattcattac gcaagagttg agcgatgaca
     8341 tgggcctttt ctgccaattc tgggttgaat accacctgaa ctaacccatc tcgatctcgc
     8401 aaatcaataa atatgagatt gccatgatcg cgtcttcggt gaacccatcc tgccaaggtg
     8461 accgattttc caatatcctg agagcgcaaa gctccacaac taatatcttt caacaattaa
     8521 accctccggc ctgccgataa gaaaatttat attcctctta atttatcttt ggggacatct
     8581 agcgtagtaa atgtctctat tttttttacc ccatctatat ttccgatttt gttttgcaaa
     8641 agttccagaa gatcagacaa tgattttgta gaaacgttaa caaaaatatc aaaagatcct
     8701 gatgtgatat taacccagcg aatctcatta atttgattga gtttcaaaac gacttgcctg
     8761 atcacaggga ggtcaacttg tattccaata atagcctcag tttccgaagc tacatagttt
     8821 gggtttagaa ccactttgat atcgataata ccttcatctt tcaagatatt gaggcgacgc
     8881 ctgatggtcc cttcactaac gcctagattt ctagcaatac tggcattaga catcctacca
     8941 tccaaactta gcgtttcaat tatttggtta tctaaatcat caaccataaa agtacccgat
     9001 caactatttg cacaaaaata ttataggctc taaaggtaca tgacaaacgt tgctttagta
     9061 gttgcacaaa atatcaattt catcctctct tgagccaaga cgttttggaa agaatatctc
     9121 tgtcaaaaac aagcctacac aatagtgtag atttcagtga tccttcacga atcatatttc
     9181 cgttcgacat aaggcgtttc gataaaagca tgatcactag caacacgcta aaaattataa
     9241 tatgtgtttg ctcttgaaag gcaaagcata agagtggcag taatccaaaa gaaataaaac
     9301 accaaaatcc gctgtcttta tacagaataa ggcgccctat aataccgaga aatatcgtca
     9361 gaacaagaat ttcttcccac atttgaaatc caatcagcaa tccaaaagct gtggaaattc
     9421 cccgtcctcc ctccaatcca atgaaaatag accaattatg gccggcaatc aaactcatag
     9481 aggcaattaa aagaaaataa gggcctagac caaggataac atcgagaaat agcagagtaa
     9541 aagttccttt tattaagcag tccagcaaac cagctatgat tccgatattt ctgccaccgt
     9601 acactgctaa attggatgct ccagcattcc tagagccctg agtcctaata tctattttgg
     9661 cccaaaacct tccaaataaa tatgaaatgg gaattgcacc tataagatag cagcagcagg
     9721 gaacagcaat aaaaacaata tattggctaa aattcacggg aaatagctag atctatctgc
     9781 ttgactgatt cttgaggggg gcctacgtct ccggggtgtg ggtcgtctct attctccgag
     9841 ttatgataat cactaccacc acatttgatt aaagaatatt tttcggctag ttcaatcaat
     9901 cgggacactt gttgagaatc ataatcacca taataaacct ctatacccat taacccggct
     9961 tcacaaagtt caggtattaa ttcaaccaaa ttctgaatac tttttcgacc agactttgaa
    10021 ttagataaaa gggggtgggc caaaacaggt aatgctccgt tgtccctaag aatttccaca
    10081 gcttctgtag gtctgaccat ctctctggga atgaaccctg gggaatcggt gttcagatac
    10141 tttttaaaag cgtctttaat atttgccgcg taccctaatt ccaccaatgc catcgcaata
    10201 tggggtcttc caacagagtc tccctttgca atattcctaa ccatttccca ggatatatta
    10261 agcccaatag agacaagttt ttcaattatc ttcttccctc tgagtaatcg gtcctttcta
    10321 aatattgaaa gcttatcttg aaaccccgca tcttgtagat ctattccata tccaagaatg
    10381 tgtacttcat cgtcaagata attggtacct agttctattc cgtttatcaa cctgattcca
    10441 aacttcgacg cacattcccg agcctcctgt aaggcggagg tggtgtcatg gtccgtaact
    10501 gctattgtct gaaggcctcg ttcaaaacaa agctcgacaa ggtcgctagg agacaaatct
    10561 ccgtcagaat gattggtatg tagatgtaaa tctactttta tatgatccat tccaatccat
    10621 ctctgggagt caggttatct gacccccagc aggtattgta aatctattag ctatgctacg
    10681 gtcttctatc ttgcgtactc aacagaccgt ttttcgcgta ttaccactac tttaatttga
    10741 cctgggtatg cgagtttttc ttcgatattc ttcacgacat ctcttgcgag agaggtagca
    10801 gaaacatcgt ctatagagtc aggttcaacc attatcctaa cctctctgcc tgcttgaatt
    10861 gcgaagcatt tttcaacacc cgcaaaatcc ccggcaactt cctccaattc ctccatcctc
    10921 ttaacatagt tctcaacagt gtctttccta gaacctggcc tagcagcact taaagcatct
    10981 gcagcggcga ctataaagga ttccactgaa ctcatttcgt catcatggtg ttctcctata
    11041 caagtagtca ccctatgcga aaccttatat ttttttgcta tatctgcacc tatttctgcg
    11101 tgaggtcctt caatctcgtg gcttagtgcc ttacctatat catgaagcag tcccccgacc
    11161 ttcgccacct ttacatttgc cccaacttca gctgccatga gacccgccag atgggctact
    11221 tccagacaat gcattagtac attctcacca tagctgaacc tatatttcag tctacctaat
    11281 aatctaatta attctggatg caggcctcta acgtcagcct caagaactgc tgattcacca
    11341 cttttccata tagtttcttc tacttcttcc tgggacttct taaccatatc ttcgattcta
    11401 gcagggtgta tcctcccgtc ggctacaagt ttagacaccg ccaacctagc aacttccctt
    11461 ctgaccggat cgaagcaaga caaagtgatc gcctcaggag tgtcatcaat tattaaatca
    11521 actccagtgt tccgctctat tgagcgtata tttcgtcctt cccgtcctat taaacgacct
    11581 ttcatatcgt cactaggaat tggcaccgta ctcactgtgg cttctgaaac aacatctgaa
    11641 gctagccttt ggatcgcctg acccaatatc attctagcct tgtcatttgc ctcatttttt
    11701 gtcgactctt ccatatctcg atacttaaga gccagttcat gacgataatc gtcctcagca
    11761 gttgccatga tgacatcctt agcgtcatcc atggtcatgt cagctacagt ttcaagtcgc
    11821 tgaatacttt gagactttac cccttctgct tcttcataga ggccttctat ctcattttca
    11881 cggtcattta atttgcgttc tcttcgctct aaattttgtg acctcttctc cgcattctct
    11941 tctctactag cgactcgacg ttctatcctt cttacttcac cacgtctctc tttgagctct
    12001 tcatctccat ttttttgaag tttcaaagct tcctcttttg cctctaagag tatcgatcgt
    12061 tgctgttcta aagcctgctc cagcatatgt tccgcttcgg agcgggcagc gactttactc
    12121 ctataggttt gaaaacgttt aaagctacta cctaaaactg taccaacaat taaactgatg
    12181 aggccggcga tcgctattgt cataatatcc atgcgatata cctcctcaat attctgtttt
    12241 tacctaggtt cccatttgga aatctaacgt tatgattata tcaggcatcc actgccacag
    12301 gtgatattgg agtacttttg attcagattc caatcaaaat ccgaaagggt ttcccttctg
    12361 agaaagcata cgcatcatct ttttttggcc accagatgat gacatttctt tcatcatttt
    12421 cctaacctgt ttaaactggt ttaaaagctg gtttacgtct tgtggggaag taccactgcc
    12481 tttggcgatt cttctccttc tactaccacc tatcatctcc ggtttggatc tttcgtctgg
    12541 ggtcatcgaa tatattatcg actcagcttt cacaaggtga ttatcatcca aatcatcagc
    12601 attgacttta cctttcatca tggagaaacc tggaaccatt tcaagtattt ggctcataga
    12661 ccccatcttt ttgactgctt gcatttggtt aagaaaatcc tcaagatcaa aagtggattg
    12721 tttaattttg cgttcaaggt ctgccatctg atcctgatca aatgtagcct gggctttctc
    12781 agcaagggtt agaacatcgc ccatacctag tattcgggag gccagtcgat cagggtgaaa
    12841 tttttcaagg gcgtctattc tttcaccgac tccgatgtac ttaaccggta tcccggtcac
    12901 tgacgtgatt gataaagcag ctccaccacg agcatcacca tccatcttcg tcaacaccaa
    12961 acccgtcaaa cctatccgat catggaaatt ttgagccaca ttaacggatt cctgtcccgt
    13021 catagcatca actactaaaa gcacttcagt tggatttatg gatttttgga tttcttccaa
    13081 ttcatccatt aattcactat cgacttgaaa acgtcccgca gtatctataa ttgtccagtc
    13141 aaaattacgg tttttaccgg cctcataccc atctatagac acttttgctg ctgaaacctc
    13201 tgtaccggcc tcatagaggc taatacctat ctgatctgct aaaaccgaca gctgctctat
    13261 agctgcaggc ctatgaacat cggccgcgac caacccaacg gattggccgg aatcttttaa
    13321 gtgtttggca agctttgcag atgtcgttgt ttttccagac ccattgagcc ccaccatcaa
    13381 aacagtatta tgtttttctt ttttagtcag ctctacagaa acaccaccga gagtttttat
    13441 cagctcatca ttagttacct taacaacatg ctggccggca gtgatgctgg ataaaatttc
    13501 ttcacctata acctgctctt taatggatgc cacaaatgat ctggccactt tgaaattcac
    13561 gtcagcttcc agcagcgctc gcctcacttc cttgagggtg gtatcaatat ctttttcggt
    13621 cagatgaccc ttgttggaaa tagatttaat taccgaagta agcctatctg tcaatgtttc
    13681 aaacataata aaacaagatt actcctagat gatttcagcg tctagtaaaa tgagaggcag
    13741 tcataacctc ttaaagatta ctatcaattt gacttgaagc taaaagtgat gggttgttat
    13801 actcaatata atacatttgt tctgaggtta ccttcatatg gaaattaatt ggctcggtca
    13861 ttcgtccgta agtattaaca gtagagattt agtcttgata gccgatcctt atgatagctc
    13921 tgatggaact ttcatgggcc cgcaacaagc tgatatcgtc accataagcc acgacagtgt
    13981 aaaacattca aacgtggatt cgataagcgg gaatcccaga gttctagacg gcccaggaga
    14041 atacgaaata aataatttct acatcaaagg gttcggtaca gcgcttacta ccgtggatca
    14101 aggttcaccc cgcaaaataa acaccattta taccattaag gctgaagggt tatcgctttg
    14161 ccacataggc gctctttcac aaaaatttac cccttcccag atcgatgaaa ttggccaaac
    14221 tgacatcctt tttgtgcctg ttaccccacc tgaggatcaa gacaacactt taatacagga
    14281 cacaataact gctctccaac ccagaatcgt ccttccaatc caatacactc ttggaaatcc
//
LOCUS       DASCNS010000037        21927 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000328385, whole genome shotgun sequence.
ACCESSION   DASCNS010000037 DASCNS010000000
VERSION     DASCNS010000037.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 21927)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21927)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21927
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000328385"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="QF838_01355"
     CDS             complement(<1..1563)
                     /locus_tag="QF838_01355"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HJO59373.1"
                     /translation="MTRFVFVTGGVVSSIGKGVCVGSIGKILKSRGMTVSLLKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGSETDLDLGHYERFVDVELTKASNLTAGQVYSDVIAKE
                     RKGEFLGGTIQVVPHVTDNIKQHILGLAESSGADVIVVEVGGTVGDIEGLPFLEAIRQ
                     MRNEVGRDNVFYMHLTFLPHISSTDELKTKPTQHSVRELRSIGIQPDAIVCRSDEKIP
                     EDLRKKIMVHCDVPLEGVLMVPTVASIYEVPLILEEQGLATLITDAMAIDVEDQKLGL
                     NGWSDLVDLIKSPKERLKIAVVGKYVDLHDSYMSVKEALIHAGLFHSRDIEIDWIGAE
                     DVENDGAAHYLSNVDGIVVPGGFGYRGVEGMIDTVKFARENNVPYLGLCLGMQVMVIE
                     AARNLLGKEGANSSEFDSDTKDPVIDLMLDQVDVKEKGGTMRLGVYPCVIEDGSITSR
                     AYGTKTIMERHRHRFEFNNAYKDDLKSVGLLPTGISPDGQLVEIVEYKSHPFMAGTQF
                     HPEFLSRPTRPHP"
     gene            1895..2803
                     /locus_tag="QF838_01360"
     CDS             1895..2803
                     /locus_tag="QF838_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004956875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HJO59374.1"
                     /translation="MRYYRLVNSTGDLCLSAETGEGLLENLTSVEDDVTDILDLIKAS
                     SITGESIDDLARRILDSGESEQISLSDIIHDNPDVDSEWAIDRPFDPPEVWAAGVTYK
                     NSELERRRESDTPDIYSKVYNADRPEVFFKSTSERCMGPFDDVGIRSDSSWDAPEPEL
                     AFVIYKGEIIGYTIGNDMSSRSIEGENPLYLPQAKMFDNSCSIGPCFATSETISTPQD
                     LSVDCVIVRNGEEVFSGSTSTSEMARTCQEICEWLLKSNFVPDMTTVLTGTSIVPPPD
                     FTLEEGDRVIISIENLGVLENNVIEV"
     gene            complement(2800..3051)
                     /gene="xseB"
                     /locus_tag="QF838_01365"
     CDS             complement(2800..3051)
                     /gene="xseB"
                     /locus_tag="QF838_01365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HJO59375.1"
                     /translation="MAKKQLDALSFEEIMDELEKSVADLENGKLPLNKASEVYEKGMK
                     LAEEANKRLSDTEIKITDIKKAFEEKIEMSSQSEDDLDI"
     gene            complement(3073..4299)
                     /gene="xseA"
                     /locus_tag="QF838_01370"
     CDS             complement(3073..4299)
                     /gene="xseA"
                     /locus_tag="QF838_01370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HJO59376.1"
                     /translation="MPALSVSQVILHLNNLFGSDPILSDVCVIGEVSRATKSASGHAY
                     FTLKDEDGVLDSALFRGGIGVEHLEIGSEVFAFGRVSIYGPSGRLQLIANLIEPAGDG
                     ILQARFEEMKNKLESEGLFDQGRKRPVSSFPSVLGIVTSKDAAAWQDIQQTIARRFPR
                     VELVLAHTPVQGNTAAPLIAESINSLNQVGGIDTIIVARGGGSPEDLWPFNEEIVARA
                     IFASAIPVISGVGHESDWSISDLVSDLRASTPTAAAEQAVPNITELMGRLDYYVSQMH
                     LYCSTKIDTGRNELKYSVAKLDRALPDLVTYKIRLDDLITRANSNIVHKNDLTKEYLK
                     GLAEKMTALAPHTTIARGYAVLQKSLDGKIVNSVDDIGSGENISITVSDGVIDAKTVE
                     DNMGLGVPEEHQLNLL"
     gene            complement(4304..4867)
                     /gene="efp"
                     /locus_tag="QF838_01375"
     CDS             complement(4304..4867)
                     /gene="efp"
                     /locus_tag="QF838_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HJO59377.1"
                     /translation="MTIGYGELKKGMSIELDGEPYSVVDYERSKMQQRAPVMRIRFRN
                     LRSGRIIDKTFSGYDVSFTPASVDRRKAQYIYPDGDFYYFMDNETFDQFPMGTEQIAD
                     SLDFMIEQTEVELIFYEDNPISLELPTTVELKVDDSPPGVKGDTAQGATKLATLETGF
                     ELQVPLFVNQGDVIKVDTRTGSYISRV"
     gene            complement(4916..5980)
                     /locus_tag="QF838_01380"
     CDS             complement(4916..5980)
                     /locus_tag="QF838_01380"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HJO59378.1"
                     /translation="MQSKYRARRDQIKKACNALNADAFLVSSLVNVRYLSGFTGSNGF
                     LLISGNNDFLFTDSRYIEQGREQTCDQITVSLSSGFSAIEEVSRLYSVTDVVFESDQL
                     TYSEFKGMEKLLNPRVNLIPSEGVVEKVRSVKDKDEIDLIRKAAYLADEAVTFAIQES
                     KPGKSEVEIAWVIEKFLRENGADGVAFDTIVATGANSAKPHHRAGSTTIKAGDSLVID
                     MGALLNGYRSDITRTVLVDGENEKFRRVYEVVLEAQITAIKAAKKGVLGKELDLVARE
                     VIANHGFGQNFSHGLGHGVGLDVHEMPMVVPSSEHILEEGMIFTVEPGIYLPGWGGVR
                     IEDMVLLEEAEVSLLTNAPK"
     gene            complement(5970..6410)
                     /gene="aroQ"
                     /locus_tag="QF838_01385"
     CDS             complement(5970..6410)
                     /gene="aroQ"
                     /locus_tag="QF838_01385"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HJO59379.1"
                     /translation="MKILVINGPNLNNLGKRDESHYGAESFSEVITSIEQFSKELGIE
                     VKFSQSNSEGELISFIQKEASTSDGIVINAGALTHYGLSLKDSLIDAHIPLVEIHISN
                     IHQREEYRRTSVVEPVAIGQVAGFGTSGYLYALELLINYLENAI"
     gene            complement(6411..7328)
                     /gene="xerA"
                     /locus_tag="QF838_01390"
     CDS             complement(6411..7328)
                     /gene="xerA"
                     /locus_tag="QF838_01390"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HJO59380.1"
                     /translation="MNSYIEAYLQHMVGYRGLSDATVKTYLQDLKTFKNFLELTGIAD
                     LRDVDRRTVRGFLVYLADKNYQRSSISRKLSVLRGFYRWLVTEDVLAVDPVPRRSTMK
                     KEVKLPRFLTNDEVVRLIDSPKSGTYSETLRNRDISLLELVYASGLRVSEVHGLNIGD
                     VDFHSKTVRVIGKGDKERLTLLGFPAKSTLQRYINLDRPKVKGFDKSDSLFLSKQGSR
                     LSIRSIQNLVKKYSRLAGLGNEVHTHTLRHSFATHLINGGADLRVVQDLLGHSSPSTT
                     QIYTHVTGVEARKSYLNAHPMAKELEEKI"
     gene            complement(7424..9565)
                     /gene="topA"
                     /locus_tag="QF838_01395"
     CDS             complement(7424..9565)
                     /gene="topA"
                     /locus_tag="QF838_01395"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HJO59381.1"
                     /translation="MAKDLVIVESPAKARTVGRFLGKDYDVKASMGHVRDLPRKKLGV
                     EIGDNGFIPTYTVTNRQIVSELKKAAATAGTVYLATDPDREGEAISWHLVEAANIDID
                     KTRRVVFHEITKPAIEEAFANTRDIDVDLVDAQQARRILDRLVGYTLSPVLWSKVKKG
                     TSAGRVQSVALRMVVDRQKEIDAFKPVEYWTIGALLGVKPKENLLEASLVSIEGSKKK
                     PEINNENDAALILTDLKGSGFVVQSYITKEGKRRPSAPFITSTLQQEASRRLRFSASR
                     TMILAQQLYEGIDMGSEGVEGLITYMRTDSTNVSGVALTEASEYIKDKYGSGYALAKP
                     RTFSKRVQGAQEAHEAIRPTSVLRTPETLRDKLDRDQYRLYDLIWKRLVASQMAEAVY
                     DRTTIDFDAKSVSSAKVYTFRTTGSVLKFDGFRVLYTEAKDDETEEEEKGLPILSSGQ
                     EMHCDELSKDQHFTEPPPKFTEASLIRNLESEGIGRPSTYASIIGTIVERDYVRRDRG
                     RFTPTKLGIAVTEFLKEHFEDIVDVGFTAGMEKQLDDIAEGLTQWNPMLQEFYGPFDQ
                     AIETAKVEAVRVPREQIDEESDQVCEKCERPMVIRGGRNGRFIACSGFPDCKNTKPLT
                     IGIKCRECNVGELAERKSKRGIFFGCTNYPDCSFAVNTKPISNPCPDCDGILLPSGRD
                     NVRCPSKECGFRGSITEFEEEPESVSAGLAS"
     gene            complement(9726..9818)
                     /locus_tag="QF838_01400"
     tRNA            complement(9726..9818)
                     /locus_tag="QF838_01400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9782..9784),aa:Ser,seq:gga)
     gene            complement(9846..9932)
                     /locus_tag="QF838_01405"
     tRNA            complement(9846..9932)
                     /locus_tag="QF838_01405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9896..9898),aa:Ser,seq:cga)
     gene            10160..10306
                     /locus_tag="QF838_01410"
     CDS             10160..10306
                     /locus_tag="QF838_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59382.1"
                     /translation="MEPFLDEGGLLFKAEFIILEPTILIAAAPIIPSGIPFKKVLRDK
                     DFFL"
     gene            complement(10404..12851)
                     /locus_tag="QF838_01415"
     CDS             complement(10404..12851)
                     /locus_tag="QF838_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJO59383.1"
                     /translation="MVLSADKFTEQAQEVIAASQDIMNRYRHNQWDSEHLFLALIEQE
                     KGVPSEIFKILGVNLEILHSTLHANLEEGNRVAGQTNQFFITPRLSELFERADEESQR
                     LNDEFIGTEHILIALAGEETGASAEILSGFKVTTEEVYRALHGIRGSHRVTDQRAESR
                     YKSLEKFATDLTLLAENGRLDPIVGRDIEIARVMQTLIRRTKNNPVMIGGAGVGKTAL
                     AEGLAQRIAAGDVPFELRSKKILALDMGSLLAGAKFRGEFEERLKAVMDEVKQSKGQI
                     MLFIDEIHTVVGAGATDGSLDASNMMKPALARGELQCLGATTEEEYTKYIESDAALER
                     RFQPILVEEPDIDTSIEMLKALRPKYEAHHRLKIQDEALSSSAKLSQRYISGRLLPDK
                     AVDLIDEAASKIRIDQQLHPMTLREKESKLRQLQIEEIASSEMGKYEKAAEIKAEIAR
                     IGENYTDERNQLNSLETSERQVTSEDIASLVATWTGIPVDRLLESEADKLLNMESRIQ
                     SRLVGQREAVEAVCEAVRRGRAGIKDENRPIGSFIFLGPTGVGKTELARSLAWYLFDD
                     EEDMVRVDMSEYMESHSVARMIGSPPGYVGYSDGGQLTEAVRKKPFKVILFDEIEKAH
                     PDVFNILLQILEDGRLTDGQGRVVDFKNTVIIMTSNLGSSLTASEPIGFLRDTDSSKH
                     SEKLRISIEDALKKSFRPEFLNRVDEILIFQPISEEDQRQIVDIMIENLRKKLVDHNI
                     DIELSEVAKSWIASDGFDREYGARPLRRSIRKNIENPLSTALINGDFDSGDHIYVDLA
                     EEKIILEKSRTYESIAI"
     gene            13035..13685
                     /locus_tag="QF838_01420"
     CDS             13035..13685
                     /locus_tag="QF838_01420"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HJO59384.1"
                     /translation="MLIDIHTHTYPTSDDSTLTPEELINRAKSIGLDGVCLTDHDRFW
                     TPSEVEKLGKDNDFLVIPGCEVTTEEGHLLVYGLTEYIFGMHKSTFVKDLVDEAGGAI
                     VVAHPYRRVYRETAPQDKASYSEMISRGLRNEVFGMVDGIEVGNGRGTDKENEFSGNL
                     AKELKMPGTGASDAHKLSDIGTYATEFYDKITGSDDLIVSIKSGRYDARKLDIRPV"
     gene            complement(13693..14667)
                     /gene="holA"
                     /locus_tag="QF838_01425"
     CDS             complement(13693..14667)
                     /gene="holA"
                     /locus_tag="QF838_01425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HJO59385.1"
                     /translation="MIVFLEGKDEFRLSERVSEFRLTVTPQEMSDINTTMLDGNIVTI
                     EELLTSLSTVPFMADRRLVIVEGLLNRLGGGDKRSAGSKKDLAEWVNLPDLLKDLPPT
                     ANLLLIERESIPSNKFLSTVLKLGQVEKFSLLRHRDLVEWINARCAKLGLDIERNAVA
                     LIADSVGSELRLIDSELNKIKIYSRGRLITREDITLMVPYVRQQNVFRVVDSVIEGRT
                     RDALNASAMLIGLGESPAGIVRMIERQLRFLILAKHLLGRKIPAGDIGKQINLSGYPL
                     QKTLEMERKISQPKIMSMHDNLLKSNIRVREGKLTEQESFDLLISELH"
     gene            14767..15084
                     /locus_tag="QF838_01430"
     CDS             14767..15084
                     /locus_tag="QF838_01430"
                     /inference="COORDINATES: protein motif:HMM:NF018508.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HJO59386.1"
                     /translation="MGRLPIIFLKLMLDRRIPIKYKLLPALGLAYVILPFDIIPDMLP
                     ILGFLDDIIILVGSIIIFMIFGPLKKLSDTIKNSNMKENRQKETDVVEGEYRIIEEPT
                     DSQ"
     gene            15200..15841
                     /gene="rplI"
                     /locus_tag="QF838_01435"
     CDS             15200..15841
                     /gene="rplI"
                     /locus_tag="QF838_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HJO59387.1"
                     /translation="MRVVFIEDVEGVALGGEIKEVKNGFARNYLIPKNLAAPATHNNL
                     QRIHKLTKDAAISRTYRLDEMKNLAETLDGTEIRIEMRAGSNNRLYGSVTGTMVADAL
                     VEETGIGIERRLVRLDDPIREVGTYEVPLRLYTDVNALIKVTVYATGTDPFEMVAEEN
                     EESNEEKLSPEGDSGSNPEDTSNSDIVEEVSAPSANHVESDSTEAESGEDSQE"
     gene            15942..17312
                     /gene="dnaB"
                     /locus_tag="QF838_01440"
     CDS             15942..17312
                     /gene="dnaB"
                     /locus_tag="QF838_01440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HJO59388.1"
                     /translation="MYTDRTLPHDIEAEHSVIGSLLLDGDSLSKVSSILKPNDFYLEK
                     NRYCFEACLNLAGRNEVINQVTVAHELSLQEQLDSIGGSVHLSDMVNEVPSPAHILHF
                     AQIVQRTSIMRQLVQAAGSIANIGYESSPDTNESLNRAEELLFGIRSERGTRDFAALR
                     DVLDNYMAESLQPDINTLAPVQSGYNQFDQLLGGGMQRSDLLIVAARPSLGKSTLAFN
                     MAAAAAANGNNVGIFSLEMSAEQIALRLLSSEAHVDSHRLRLQLINEMEEQRLFDAIG
                     NLSDLPLYIDDTPFQTIVEMRSKARRLQAERGLDLIIVDYLQLINGSGGRNANRVQEM
                     GEISRSLKGMARDLNVPVLACSQLSRAIEQRPDHRPMLSDLRESGSIEQDADVVAFIH
                     REDRYVDPEAWEKRYPTQEYPANIADIIVAKHRNGRTGGIKLYFQERFVKFENLEIYQ
                     QDSVSA"
     gene            17312..17965
                     /locus_tag="QF838_01445"
     CDS             17312..17965
                     /locus_tag="QF838_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HJO59389.1"
                     /translation="MAFQGFIKGVRYIPVPAPVFGELLQEIQDITELKIILRIIRLLH
                     NKKGLERYVSVDELLADRVLSIGLGSMDLNNRKTNIIQSLESSTKRKILIKINRKDIG
                     TSAYFLNTEFERQSMKKMPDLEHQSDFEPWETGEDRPNIYSLYEQNIGILTPIVAEKI
                     GEAEQKYPVEWIEEAIAEAVSLNSRNWRYISRILERWEIEGKQNGESRKHSGKARYI"
     gene            17922..19274
                     /locus_tag="QF838_01450"
     CDS             17922..19274
                     /locus_tag="QF838_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012234.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJO59390.1"
                     /translation="MENLGSILAKLDTFKANRSNDLSKDIDLDPPINCDICNDKGWLT
                     PKLPTGHQDFGSIKSCACRKNVKQEEMTTRLLSYSNLGSLSRYTFETLDLKNEPTQQN
                     GGDVFREAFVNCSNYSEHPSGWILINGPHGSGKTHLSAAIANRCISTGKPAFFIYVPD
                     LMDHLRASYSNNLDFEYDDLFDQVKNAPILILDGITDHSVSSWSAEKLNQILNHRSNG
                     RLPTVLTTSEEIDSLDPFIKSRIYDSNLCDIVTTDSRISNYDEISSLGAIPKELSQMN
                     FDTFNPKGRRGNSNLDPVYESAKKYSQTPDGWLTFYSDNSGVGKTHLAVAIGNYRKSI
                     GEDVFFAFVPELMDELRSGFSPDSSLNSSRIFDRVKDSSLLILDDLGNEQDTDWSQKT
                     IYHLLVYRHNHRLPTIITTRTDFTDIAGRSAQASRIQDAKIGQILNIQAPDYRMGRTR
                     "
     gene            19296..19661
                     /locus_tag="QF838_01455"
     CDS             19296..19661
                     /locus_tag="QF838_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013401.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HJO59391.1"
                     /translation="MTIDQDCIFCKIISGQEPATFHHEEKDFVVFENNLKWFSTQFLF
                     VPRLHMNQSNLWSSKNLISDIGFLANEIGQKRCPSGYRILSNFGVDGMQSQEHAHLHL
                     VGGSKLGMYVWGKLRKNPL"
     gene            complement(19752..20807)
                     /locus_tag="QF838_01460"
     CDS             complement(19752..20807)
                     /locus_tag="QF838_01460"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HJO59392.1"
                     /translation="MNSQKSHVQGLGFSSNHLFSPLELVDRFPRSDEAASTVQNARRT
                     IKDILHGDDDRVMIITGPCSIHDEEAALDYARRLIELRNRLSKKIFVVMRVYFEKPRT
                     TVGWKGLINDPGLDETFDISQGLQRARRLLQEINDMGMPAACEFLDPVVPHYFEDLVS
                     WAAIGARTTESQTHRQMASAIGIPVGFKNGTDGSFETAVDGILAARNQHSYVGTGPDG
                     HVCVLRTDGNPDGHLVLRGGKLGPNYDSESIKLASEVLSNGDLDQRLVIDCSHGNSNK
                     DHERQPSVFEDVIAQRAGGNNSIVGVMLESNINSGSQKFGNDPGTLEYGVAITDACVG
                     WDKTEEIMLWADNALSS"
     gene            21099..>21927
                     /locus_tag="QF838_01465"
     CDS             21099..>21927
                     /locus_tag="QF838_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HJO59393.1"
                     /translation="MSDIHSEMSPCCTASRESKLPFHPSKIKNTPKSQTKNINPENMA
                     TIPGGTFQMGTDYEFSFPGDREGPIKKVSLNSFLIDITAVTNKNFADFAKETKYKTDA
                     EKFGWSFVFYQFISPKNAGSANQSPPGTPWWRKVDGASWKHPEGSGSNIKTRMNHPAV
                     HISWNDATEFASHYGKRLPTEAEWEFAARGGQEQRLYPWGDELHPGGQHMCNIWQGEF
                     PTTNSKDDGFTGTAPAKRYPPNGYGLYNVVGNVWEWQHDWFSNSLNDIDAKHNPKGPN
                     "
BASE COUNT         6413 a         5094 c         4113 g         6307 t
ORIGIN      
        1 gggatggggc ctggttggac gagacagaaa ttccgggtga aactgtgtgc cggccatgaa
       61 cggatgggat ttgtattcta cgatctcaac caactgcccg tctggtgaaa ttcctgttgg
      121 gagtaatcca accgatttaa gatcatcttt ataggcatta ttaaactcaa accggtggcg
      181 atgtctttcc attatagttt tggtgccata tgctctggaa gtgatacttc catcttcgat
      241 aacacacggg tatacaccca gcctcatcgt tccacctttt tcttttacat caacctggtc
      301 caacataaga tctataacgg ggtcttttgt gtctgagtcg aattcagatg aatttgcccc
      361 ttctttgccc aataaattac gagcggcctc gataaccatg acctgcattc caagacataa
      421 tcccagatag ggcacgttat tttctctggc aaatttcact gtgtctatca tgccctcaac
      481 ccctctatac ccaaacccac ccggaacaac tatcccgtca acatttgaca gataatgggc
      541 tgcgccatcg ttctcaacat cttcagcccc aatccaatct atttcaatat ctctgctgtg
      601 gaataaacct gcatgtatca aagcttcctt gacagacata tacgaatcat ggagatccac
      661 atatttccca acaacagcaa tttttaatcg ttctttgggg ctttttatca aatccaccaa
      721 atctgaccaa ccatttaacc caagtttctg atcttcgacg tcaattgcca tagcatcagt
      781 gatcagagtg gccagacctt gctcttcgag tatcaacgga acttcatata tgctggccac
      841 cgtagggacc attaagaccc cttccagcgg tacatcacaa tggaccatta ttttctttct
      901 caaatcttca ggaatcttct catcgctccg acaaacaata gcgtcaggtt gaatgccaat
      961 gcttctaagt tcccttacgc tgtgttgtgt gggttttgtt ttaagttcat cagtggaaga
     1021 aatatgtgga aggaatgtga gatgcatgta gaagacgtta tctcgaccaa cttcattcct
     1081 catttgcctt atagcctcaa gaaacggtaa cccttcgatg tcaccaacag tacctccaac
     1141 ctccaccacg atcacatctg ccccacttga ttcggccaaa cccaatatat gctgttttat
     1201 attatctgta acgtgaggca cgacctgaat tgtgccaccg aggaattctc ctttccgctc
     1261 cttcgctatc acatcgctat atacctgacc tgctgtgagg ttggaagctt tggtcaattc
     1321 gacatctaca aatctttcat aatggcccaa atcaaggtca gtttcactcc cgtcatctgt
     1381 gacaaacacc tcaccatgtt ggtaaggaga catggtgccg ggatcaacgt ttaagtaagg
     1441 atctaacttc aataaagaaa cagtcatacc tctagatttg agtattttgc caatagaacc
     1501 aacgcaaacc cccttgccta tcgaactcac taccccgcct gtcacaaata cgaatctagt
     1561 cattaataaa ccttttaagt taagaaaaaa gagcggtcaa tttaaacttc cataggccag
     1621 attaacgagg ctggattgtc aacaccaatt tggcaacctc ttgcggaatg attacaaatt
     1681 tgagtaggac agacatctac aggaagagtt cccagttcaa gaattgtccc aaaatttgaa
     1741 tcctggcggt aaacgataac ccagtatccc ctttgtcagt ttctgtagta tagtcaccct
     1801 cttttttcta tgtcaaacaa ggagcctttt aacacaatcg acatcgatct tggtagaatt
     1861 tgtaaaacac cttaaatcag gaggttgaat cactttgcgt tactatcgat tagttaactc
     1921 cactggagac ctgtgtttat cagcggaaac aggtgaaggg ttgctagaga atctgacctc
     1981 tgtggaagat gatgtcactg acattttgga cttgatcaaa gcatcctcta tcactggaga
     2041 gtcgatagat gacttagcaa gaagaattct agattcaggg gagtcggaac aaatatcgct
     2101 ctcagacata attcatgaca accctgatgt cgatagcgag tgggcaattg atagaccttt
     2161 tgatccacca gaagtttggg ctgccggtgt tacatacaaa aacagcgaat tggaacgccg
     2221 ccgtgaaagt gacacacctg atatatattc gaaagtttat aacgcagata gacctgaagt
     2281 tttttttaaa tccacctcgg aaagatgcat gggaccattc gatgatgtgg gcatacgatc
     2341 tgattcctcg tgggacgcgc cagaaccgga gttagccttt gtgatatata aaggcgagat
     2401 tataggttat acgatcggca acgacatgag cagcaggtca attgaaggag aaaatccact
     2461 ctacctaccg caagcaaaga tgtttgataa tagttgttct ataggacctt gttttgccac
     2521 gtcagagact atttcgaccc ctcaagatct cagtgtagat tgtgtgatag taaggaacgg
     2581 ggaagaggtg ttttcaggat ccacttcaac atctgaaatg gcaagaacct gtcaggaaat
     2641 atgtgagtgg ttactcaaaa gcaatttcgt cccagacatg accaccgtcc tgactggtac
     2701 atcaatagta ccaccgccag attttactct tgaagaggga gatagggtga taatatcaat
     2761 agaaaatctg ggtgtattag aaaacaacgt tatcgaggtc taaatatcta aatcgtcttc
     2821 actttgagaa ctcatctcga ttttttcctc aaacgctttt ttgatgtcgg taatctttat
     2881 ctcagtatca gatagtcttt tatttgcttc ttccgctaat ttcatccctt tctcataaac
     2941 ctctgaggct ttatttaaag gtaatttccc gttttccaaa tctgcaacag atttttccaa
     3001 ctcatccatt atctcttcaa aagataaagc gtccaattgt tttttagcca caagtcctcc
     3061 aaaagccaat ttttataaaa gattaagttg atgttcctct ggaactccga gtcccatatt
     3121 atcttcaact gttttggcat caatgacacc atcactcact gtaattgaga tattttcgcc
     3181 actaccaata tcatctacac tgttaacgat cttaccgtcc aaggattttt gaagcacagc
     3241 gtatcctctt gctatggttg tatgaggagc tagagcagtc attttttcgg ccaatccttt
     3301 caagtattcc ttggtcaaat catttttgtg aacaatgttt gaatttgctc tggttatcaa
     3361 atcgtcaagt cttatcttat atgtcacaag atctggcagg gcacgatcaa gcttggccac
     3421 cgaatatttc aattcattcc gcccagtgtc aatcttagtg gaacaataaa ggtgcatctg
     3481 agatacatag tagtctagcc tacccattag ttcagttata tttggaaccg cctgctctgc
     3541 ggcagccgtt ggagtagatg ctcttaagtc agacactaaa tctgatatag accaatctga
     3601 ctcatgtcct accccactaa tcaccggaat agcagaagca aatatagctc tcgctacaat
     3661 ctcttcatta aatggccata aatcctctgg ggagcctcct cctctggcca caatgattgt
     3721 atctatccct cctacctgat ttagcgagtt tatagactca gctatcagtg gagccgctgt
     3781 gttcccctga acaggagtat gcgccaatac tagttcaact cttggaaatc ttcgagcgat
     3841 cgtctgctga atatcctgcc atgctgcggc atccttagag gtgacgatcc ccaatacaga
     3901 ggggaaagaa gaaacgggcc tctttctccc ttgatcaaaa aggccttcag attccaactt
     3961 gtttttcatc tcttcgaacc tggcctgtag gatgccatcc ccggctggtt ctattagatt
     4021 agctatgagc tgcaacctgc cacttggacc atatattgag actctaccaa atgcgaaaac
     4081 ctccgaacct atttccaaat gttcaacacc gatcccccct ctgaataagg cagaatccaa
     4141 cactccatcc tcatccttca aggtgaaata tgcgtgtcca gatgcggact ttgtagctcg
     4201 agaaacctct ccaattacac atacatcact taggatcggg tcggatccga agaggttatt
     4261 gaggtgcaat ataacttgtg aaacacttaa agcaggcatt cggctaaacc ctagaaatat
     4321 aacttccggt cctagtatct actttgataa catcgccttg atttacaaaa aggggcacct
     4381 gaagctcaaa ccctgtttct aatgtagcca atttagtcgc cccctgcgct gtatcgcctt
     4441 taactccagg aggagagtcg tcaactttca attcaactgt ggttggtaac tccaaagaaa
     4501 tgggattatc ttcataaaaa atcaattcca cctctgtttg ttcgatcatg aaatctaagg
     4561 aatcggcgat ttgctctgtg cccatcggaa attgatcaaa tgtttcattg tccatgaaat
     4621 agtaaaaatc tccgtcagga tatatatatt gtgcttttct cctatctaca gacgcaggag
     4681 tgaagctcac gtcgtaccct gagaaagtct tgtcgataat ccgaccagat ctcaaatttc
     4741 tgaaccttat cctcatcacc ggggctctct gctgcatctt acttctttca taatccacaa
     4801 ctgagtatgg ttcaccatcg agttctatcg acatcccttt ttttaattca ccatatccta
     4861 tcgtcattgt gataaccccc atatgaaaaa tatgtagtgc aaagaacctg tctaactatt
     4921 ttggagcatt agttaataaa gatacttcag cttcctcgag caataccatg tcttctattc
     4981 taactccacc ccatccaggc agatagatgc caggttctac agtaaagatc atcccttctt
     5041 ccaaaatatg ctcagaggaa ggaactacca ttggcatctc atgcacatca agaccaacac
     5101 catggcctaa tccatgtgaa aaattttgcc cgaatccatg gtttgctatt acctcccggg
     5161 ctaccaggtc caattcttta ccaagcacac ctttcttggc agcctttatc gcagtgatct
     5221 gagcttccag aaccacctca tatacacgcc gaaatttctc gttctcaccg tcaactaaaa
     5281 cagttcgtgt tatatcgctt ctatacccat ttagcaaagc gcccatgtct ataactagcg
     5341 aatcgcctgc tttaatagtt gtgcttcctg ccctatggtg cggtttagca gaattagcac
     5401 ctgttgcgac tatggtgtca aaggcgacac catcagcccc gttctccctc aagaattttt
     5461 ctataaccca tgctatttca acctcagatt tacctggttt tgattcttga atggcaaatg
     5521 ttacagcttc gtcagcaaga taagctgctt ttcgaatcag atctatctca tctttatctt
     5581 taacagatct gactttttct actaccccct cagagggaat caaatttact ctagggttca
     5641 gcaatttttc catacctttg aattcagagt aggttaattg gtctgattca aatacgacat
     5701 cagttacgga atataatctt gacacttcct ctatcgcact aaatccagac gacaaggaaa
     5761 ctgtaatttg atcacaggtc tgctccctac cttgctctat gtacctagag tcggtaaaaa
     5821 gaaaatcatt atttccagaa atcaatagaa atccattgct tcccgtgaat ccgctcaagt
     5881 aacgaacatt caccaaagat gacactaaga aagcatccgc atttaaggca ttgcaagctt
     5941 tctttatctg atcccgtcta gcccgatatt tagattgcat tttcaagata attgatcaaa
     6001 agttccagag catacaaata tccagaagtg ccaaacccgg ccacttgccc aatagcaacc
     6061 ggttccacta cagaggttct cctatattct tcacgctgat gtatgttgga tatgtgtatc
     6121 tccactaaag gaatatgagc atctatcaat gaatccttaa gagataatcc ataatgagtc
     6181 aaagctccgg cgtttatcac aataccgtct gaggtagatg cttccttttg aataaaacta
     6241 atcagttccc cttccgagtt actttgggaa aattttacct cgatcccaag ctccttcgaa
     6301 aattgttcaa tagatgtaat aacctcacta aatgattcag ccccgtaatg agattcatcc
     6361 cttttcccca aattattcag attaggtcca ttgatgacca atattttcac ttaaattttc
     6421 tcctctaact cctttgccat agggtgtgca ttcaaataac ttttccttgc ttcaacacct
     6481 gtgacatggg tatatatctg agttgtagag gggctggaat gtcccagcaa atcttggaca
     6541 accctgagat cggctcctcc gtttattaga tgagttgcaa atgaatgtct taaggtatga
     6601 gtgtgaactt catttcctaa accggcgagc cgtgaatatt tttttaccaa gttctgtatt
     6661 gacctaatac taagcctaga cccttgttta cttaagaata atgaatcact tttatcaaag
     6721 cccttaactt tcggtctatc caaatttatg tatctttgta gggtagattt agcaggaaat
     6781 ccgagcaatg tcagtctctc tttatcaccc ttaccgatca ctctaacagt cttggaatga
     6841 aaatccacat cacctatatt tagtccatga acctcactta cccgcaggcc tgaagcataa
     6901 actagctcca aaagagaaat atctctattt ctcagtgttt cagaatacgt ccccgattta
     6961 ggtgaatcaa ttagcctgac aacctcgtca ttggtcaaaa atcggggtaa tttcacttcc
     7021 tttttcatgg tagaacgcct aggaacagga tctaccgcca acacatcttc tgttactagc
     7081 cacctataaa atcccctaag gactgagagt tttctagaga tactagatct ttggtaattt
     7141 ttatcggcca aatataccaa aaagcccctc accgtcctac ggtccacgtc tcttagatca
     7201 gcaattcctg tcagctcaag aaaattcttg aatgttttta aatcttgtaa ataggttttg
     7261 actgtcgcat ctgacaagcc gcggtagcct accatatgct gtaaataagc ctcaatatat
     7321 gaattcatgt ctttctcagt tggttcatat cccataaaaa taggatatta acgtctctcg
     7381 atatttacca gcaattaaga cactgattat tcgctatact ggattagctg gctaacccgg
     7441 cactaacgct ctctggctct tcctcaaatt ctgtaataga tccccgaaat ccacactctt
     7501 ttgacggaca cctaacgtta tctctgcctg aaggaagtaa tattccatca cagtcgggac
     7561 acgggttgct aattggtttg gtatttacag caaaactgca atctgggtag ttggtacaac
     7621 cgaagaaaat ccctcttttg cttttgcgtt ctgctaactc gcccacatta cattcacggc
     7681 attttattcc tatggtcaaa ggtttagtgt ttttacaatc tggaaaccct gagcaagcga
     7741 taaacctacc gtttcttcct cctcgtataa ccatgggcct ctcacacttt tcacatacct
     7801 ggtcgctctc ctcatcaatc tgttccctag ggactctcac agcctcgact ttagctgtct
     7861 caatcgcttg gtcaaacggc ccataaaatt cttgcagcat aggattccat tgcgtcaagc
     7921 cttcggctat gtcatcaagc tgcttttcca tacccgcggt aaaaccaaca tctacaatat
     7981 cttcaaaatg ttcctttaaa aactcggtga ccgcaattcc caatttagtg ggggtaaatc
     8041 ggcctcggtc tcggcgaaca tagtctcttt cgacaatagt acctattatc gaagcataag
     8101 tacttggtcg accaattcct tcactctcca aatttcttat taaagatgct tccgtaaact
     8161 ttggaggtgg ctctgtaaaa tgttggtctt tgctaagttc atcacagtgc atttcctgac
     8221 cactggaaag aattgggaga cctttttctt cctcttcggt ttcatcgtcc ttagcctctg
     8281 tataaaggac tctaaaaccg tcgaatttca agacagatcc agtagtcctg aacgtataaa
     8341 ctttcgctga tgacacactt tttgcatcaa aatcgatggt agtcctgtca tatactgcct
     8401 cggccatttg acttgctacc aaccgcttcc atataagatc gtataaacga tattggtctc
     8461 tgtctaattt atcccgtaat gtctccgggg tcctcaacac tgaagtcggt cttatagctt
     8521 catgcgcctc ttgggcaccc tgtactcttt ttgaaaaagt cctaggtttt gcgagagcgt
     8581 aacctgaacc atacttgtct tttatatatt cactcgcctc agtaagcgct acacctgaaa
     8641 cattagtcga gtccgtccgc atgtaggtta tcaacccctc aaccccttcg gatcccatgt
     8701 cgatcccctc atagagttgc tgggcaagta tcatcgtcct tgaggctgaa aaccttaatc
     8761 ttcgggaggc ttcctgctgc aaagtgcttg taataaaagg agctgagggt cttcgtttac
     8821 cttctttggt tatataactt tgaactacaa atccagaacc tttcaagtcg gtaagtatta
     8881 aagcagcgtc gttttcattg tttatttcag gcttcttttt ggacccttct attgagacca
     8941 aagaagcttc cagcaagttt tctttaggtt ttacccccag taaggctccg atagtccaat
     9001 attccaccgg cttgaatgcg tctatttcct tttgccggtc aacaaccatt ctcaaagcga
     9061 cagactggac tcggccagca gaagtacctt tctttacctt gctccacaac actggactca
     9121 acgtgtaccc gaccaaccta tccaggatcc gtctagcttg ttgagcatct accaaatcca
     9181 catcaatatc tcttgtgttc gcaaaagcct cttcaatagc cggtttggtt atctcatgga
     9241 agacaactct tcgagtttta tcaatatcaa tattggctgc ctcaacgaga tgccaagaaa
     9301 tagcttcgcc ctctctatca gggtccgtgg ccaagtaaac tgtaccggct gttgcagccg
     9361 ctttttttaa ttcggaaacg atttgtcggt tggtgacggt gtaggtcgga ataaacccgt
     9421 tatccccaat ttccacacct aattttttcc taggcaggtc tcttacatgc cccatagagg
     9481 ccttcacatc ataatcttta cctagaaacc tacccactgt cctagctttt gcaggagatt
     9541 caactatcac aaggtcttta gccattactc tttatcacca aaattacatt aataaacgac
     9601 ttaattaaac aaaattttca ataagtataa attgcaggcc ctaataatac tttaacgaaa
     9661 cactcaatcg tcaataacaa attattatct gtcaacgaat atccatacaa ccaaaatgct
     9721 ggtcatggcg gagagagtgg gattcgaacc cacggttccc cttgtgaggg aacacgcgat
     9781 ttccagtcgc gcctattcgg ccactctagc atctctccat attgactgtt ccggttccgc
     9841 tgcggcggag agagtgggat tcgaacccac gatgagatta ctcccatact ggttttcgag
     9901 accagcgcct tagtccgctc ggccatctct cccaactctg acaaacgagt atatcagtcg
     9961 atttaaaaca attgaaatct taacccgttt gaaaaaggaa gggattccga aactagctgc
    10021 catctccttc cacacatccc agcaacctac gatcagtcca ctatccagtc cacaattttt
    10081 cgcctgctaa atttattgat acaaacccca agatcccaag gttagcatct agtgagtcta
    10141 agcatctttt ggagaaaaaa tggaaccttt tttagatgaa ggaggcctcc tattcaaagc
    10201 tgaattcatc atcctcgaac ctacaatact tattgctgct gctccgatta tacctagcgg
    10261 tatacctttc aaaaaagttc tccgtgacaa agactttttc ctttgagatg cctggctcat
    10321 ataaccacct taaaataatt tacttagttt ccgttcaaat tataaggata ccaatctaat
    10381 ggatcaagaa taaatcacaa atgttaaatt gcaatcgatt cgtaagttct actcttttca
    10441 agaataattt tttcttccgc taaatcaaca tatatgtggt ctccactatc aaaatcccca
    10501 tttattaggg ctgttgacag aggattttca atattttttc tgatgctgcg cctgaggggc
    10561 ctagcgccat attctctgtc aaatccgtca gaggcaatcc acgatttggc gacttcggac
    10621 aactcaatat cgatattgtg atccacaagt ttttttctta ggttttctat cataatatct
    10681 acaatttgcc tttgatcttc ttctgaaata ggttggaata tcaaaatttc atcgaccctg
    10741 ttcaggaatt caggcctgaa ggattttttt aaggcatctt ctattgaaat tcttaacttt
    10801 tcgctatgtt tcgagctgtc cgtgtctctc aaaaaaccta tgggctcaga agcggtaagt
    10861 gacgacccta gattactggt catgataatt actgtgtttt taaaatctac tactctacct
    10921 tggccatcag taagccttcc atcttcgagt atttgcagca gtatattaaa aacatctgga
    10981 tgggcttttt ctatctcatc aaaaagaata actttgaaag gctttttcct cacggcttct
    11041 gtcaattgac cgccatcaga ataaccaaca tatcctgggg gggagcctat cattctagct
    11101 actgagtgag actccatata ttcactcata tccacacgta ccatatcttc ctcatcgtca
    11161 aataagtacc atgcgagtga cctagccagc tcggtcttgc caactcccgt cggtcctaag
    11221 aatataaagc tccctattgg tctattttcg tccttaatcc cagcacgccc tctcctcaca
    11281 gcttcacaaa ctgcttcaac agcttctcgt tgaccaacga gccgactttg aatgcgagat
    11341 tccatgttaa gaagtttgtc tgcctcactt tctaacaacc tgtcgactgg tatccctgtc
    11401 caagtagcaa cgagagaagc tatatcctcg gaagttactt gccgttcaga ggtttcaagg
    11461 ctgtttaatt ggtttctctc atcagtataa ttttctccga ttcgagctat ttccgccttt
    11521 atttcggctg ctttctcata cttccccatc tcagaggatg ctatctcttc tatctgtagc
    11581 tgacgaagtt tagattcttt ttctctcaaa gtcattgggt ggagctgctg gtcgattcga
    11641 attttacttg ccgcctcatc tatcagatca actgccttat ctggcaataa cctcccagat
    11701 atatatcttt ggctcaactt cgccgaggaa cttagagctt catcttgtat tttcagcctg
    11761 tgatgggctt catatttggg gcgcagagct ttcagcatct ctatacttgt gtctatatct
    11821 ggctcttcaa ccaaaatcgg ctggaatctt ctttcaagag ctgcatcgga ctctatgtat
    11881 ttcgtgtatt cttcctcggt tgtggcccct agacactgca gttcacctct ggcaagagca
    11941 ggtttcatca tgttactggc atcaaggcta ccgtctgtag ccccagcacc aaccactgta
    12001 tgaatttcgt cgataaagag cataatttga cccttcgact gtttgacttc gtccataaca
    12061 gcttttagcc tctcttcaaa ttcaccgcga aattttgccc cagcaagcaa ggatcccata
    12121 tctaatgcaa gaattttctt ggagcgaagt tcaaagggaa catctccagc ggctatcctt
    12181 tgggctaggc cctctgctaa agctgttttg cctacaccag ccccaccgat catcactggg
    12241 ttattttttg ttctccgtat caaagtttgc atgactctag ctatctctat atcacgccca
    12301 acaatagggt ccaagcgacc attttcagcc agcaaagtca aatctgtagc aaatttttct
    12361 aaagacttat atcgactttc agccctttga tcggtaactc tatgggatcc ccgaatacca
    12421 tgaagagctc tgtaaacctc ttcagttgtt actttaaaac cgctgagtat ttcagctgat
    12481 gctcctgtct cttccccagc aagtgctatg agaatgtgtt ctgtcccaat aaattcatca
    12541 tttaaccttt gagattcctc gtccgccctt tcaaacaatt cagataatct gggagtaata
    12601 aaaaattgat ttgtttgacc ggccaccctg ttaccctctt ccaaattggc gtgaagagtg
    12661 gaatgcaaaa tttctagatt cacgcccaaa atcttgaaaa tttccgaagg gacacctttt
    12721 tcctgttcta ttaaagccag gaacaaatgt tcagaatccc attggttgtg tctatacctg
    12781 ttcataatat cctgagaggc ggctatcacc tcttgggcct gttcagtaaa cttgtctgca
    12841 cttaaaacca tgttatcgtc cccactacaa atactgtttt catcgttcga cttatatatt
    12901 atcaataagg ttgatacggt gcaactcaag tttatctaat gtaggcactg atacactttg
    12961 tttaccactg attctaagtt attttgagaa acccatagca ttgccgccga taacatgtaa
    13021 tacaatcaat agacatgcta atcgacattc acacccatac ttacccaacc tcagacgaca
    13081 gcaccctcac cccagaagag ctcattaatc gagcaaaatc gattggattg gatggagttt
    13141 gtttaactga tcacgaccga ttttggaccc cttcagaagt ggagaaactc ggtaaagata
    13201 atgattttct agtaatacca gggtgcgaag tgaccactga agagggtcat ttattggtat
    13261 acggtttgac cgaatatatt ttcggaatgc acaaatcgac ttttgtaaaa gaccttgtag
    13321 atgaggcagg cggggctatt gtggtcgctc atccataccg gagggtgtat cgtgaaactg
    13381 cacctcaaga taaagcttcc tattccgaaa tgataagccg tggattgaga aatgaggtgt
    13441 ttggcatggt ggacggcata gaagtaggaa acggcagggg aacggacaaa gaaaatgaat
    13501 tttctggcaa tttggccaag gaacttaaaa tgcctggaac tggggcaagc gatgcgcaca
    13561 agctatccga tataggcacc tatgccacag aattttacga caaaattact ggatcggatg
    13621 atctcattgt ttcgataaaa tccgggagat atgatgctag aaaattggat attcgccccg
    13681 tataacaggt agttaatgaa gttccgatat cagtaagtca aaggactctt gttctgttaa
    13741 ttttccttcc ctcacacgaa tattagattt caaaagatta tcgtgcatac tcataatctt
    13801 cggctgtgaa atttttcttt ccatctccag agtcttctgc aagggatagc cagacaaatt
    13861 aatttgcttt ccaatatcac ctgctgggat tttccgaccc aatagatgtt tagcaagtat
    13921 aagaaatcga agttgccttt ctatcatcct gacaatccca gcaggtgatt ctcccaaacc
    13981 tataagcatt gccgaagcat tcagggcatc tctagtccga ccttctatta cagaatcgac
    14041 aactctaaag acattttgct gtcgcacgta aggcaccata agggttatgt cttctctagt
    14101 tattaaacga ccacgcgagt agattttgat tttatttaat tcagaatcga ttaaacgcaa
    14161 ttcacttcct acagaatccg ctataagagc tactgcgttg cgttcaatat cgagacccaa
    14221 tttcgcacaa cgggcattta tccattccac taagtcccga tgcctcaaaa gtgaaaattt
    14281 ttcaacctgc ccgagtttaa gaacagtgga caagaactta ttagaaggta ttgattccct
    14341 ttcaattagc agtaaattgg ctgtaggagg cagatccttc aacaaatctg gtaaattaac
    14401 ccactcagct aaatcttttt ttgaacccgc agatctttta tcgccaccgc ccagcctatt
    14461 aagtaatcct tccacaataa ctaaccgcct atcagccata aagggaactg tggataatga
    14521 tgtcaacagc tcctctatgg ttacaatatt cccatctaac atggtggtat taatatctga
    14581 catttcctgc ggggttactg ttaggcgaaa ctccgatact cgctctgaaa gcctgaattc
    14641 atcttttcct tccaaaaaaa ctatcaattt ttcacctaaa tgataacctc aagaagtttt
    14701 aaatccagaa atataataaa atctaagaaa gacataaata aataaataac acccaaagcg
    14761 aaaaacatgg gacgactacc aataattttc ttaaaattaa tgcttgatag aagaatccct
    14821 atcaaataca agcttctacc cgccctaggt cttgcttacg tcatcttacc atttgacatc
    14881 atccctgaca tgctgcccat cctagggttt ttagatgaca ttattatcct cgtgggctcc
    14941 ataataattt ttatgatttt tggcccacta aaaaaattat ccgatacaat aaaaaattcc
    15001 aacatgaaag aaaatcgaca aaaagaaact gatgtcgtag aaggggaata tcgcatcatt
    15061 gaggaaccta ctgactccca gtaattcagt aaaaactata agcctgtatg ttataattcc
    15121 cgctcttgtc aaacacgaag aaccaaacac aaacccgtca taaatacgat ccgacttatg
    15181 ggttatagga atattaataa tgagagttgt ctttattgaa gatgttgaag gggttgcgtt
    15241 agggggagaa attaaggaag tgaaaaatgg gtttgcgagg aattacctta ttcctaaaaa
    15301 tcttgccgca cccgccaccc acaacaacct acaacgaatt cataaattaa caaaggacgc
    15361 tgccatttcc agaacgtatc ggctagatga aatgaaaaat ttggctgaaa ctttagatgg
    15421 cacagaaata cgcattgaaa tgcgagctgg atccaataac cggctttacg gatctgtgac
    15481 tgggactatg gtagctgatg cattggtaga agagactggc ataggaattg aacgaagatt
    15541 agtgcggctt gatgatccga taagagaagt cgggacatat gaagttcccc taaggctata
    15601 caccgatgtg aatgctctaa ttaaagtcac cgtttacgcc accggtaccg acccttttga
    15661 gatggttgct gaagaaaacg aagagtcgaa tgaagaaaaa ctgtcacccg aaggtgattc
    15721 gggtagtaat cccgaagata cctcgaactc agatatagtt gaagaggttt cggcaccttc
    15781 cgctaatcac gtcgaaagtg atagtacaga agccgaatca ggggaagact cccaagagta
    15841 accagaaacc gaattgtcga tagcaattcg acttgattgc gtggaagtga tcatgtcata
    15901 atcgacagcc catacgccaa attggacccc gggagattgg gttgtacacc gatagaactc
    15961 tgccacatga tatcgaagct gaacactcag tcataggatc tttgttacta gacggagatt
    16021 ccttgtcaaa agtatcttct atattgaaac caaatgattt ttacctagaa aaaaaccgct
    16081 actgttttga ggcttgtttg aatttggctg gcagaaatga ggtcataaac caagtaactg
    16141 tagctcatga attatctctc caagaacaat tggattctat aggagggtcg gttcatctat
    16201 cagatatggt caatgaggta ccaagtccag ctcacatact gcatttcgct cagatagtac
    16261 aaagaacatc cattatgcgg cagcttgtgc aggctgccgg ctcaattgcg aacatagggt
    16321 atgaaagttc cccagacacc aacgaatcat taaaccgggc agaagagctt cttttcggca
    16381 ttcgttctga acgaggaaca agggatttcg cagcattacg tgacgtccta gataactaca
    16441 tggccgaaag ccttcaaccg gacataaaca cgttggcacc tgttcaaagt ggttacaatc
    16501 aatttgatca acttttggga gggggaatgc agcgatctga tctattgata gtggctgcga
    16561 ggcctagcct cggaaaaagt acgctggcct ttaacatggc agcagcagca gcggctaatg
    16621 gtaataatgt tggtattttc agtctagaaa tgagtgccga acagatagct ctccggcttc
    16681 tttccagtga agcgcatgta gatagccatc gattgcggct tcagttaatc aatgaaatgg
    16741 aagagcaaag actctttgat gcaattggta atctttccga cctccctcta tatattgacg
    16801 acactccttt tcaaacgatc gttgagatgc gtagcaaagc cagacgcctc caggcggaga
    16861 ggggccttga tttaataata gtggactatt tacagttaat aaatggcagt ggtggtagga
    16921 atgccaacag ggttcaggag atgggtgaaa tatcaagatc attgaaggga atggcgagag
    16981 atctcaacgt gcctgtattg gcttgctcac aactaagtcg agccattgaa cagcgtccag
    17041 atcaccgacc catgttatct gatttacgag aaagtggcag catagagcaa gacgctgatg
    17101 tagttgcttt catacatcgt gaagacaggt atgttgaccc tgaggcttgg gaaaaacgat
    17161 atcccactca agaatatcca gccaacattg cagacataat agtggccaaa catagaaatg
    17221 gtcgaacggg gggaataaag ctttattttc aagaacgttt tgttaaattt gagaatttag
    17281 aaatttatca acaagactca gtaagtgcat aatggcattt caaggtttca taaaaggagt
    17341 gagatacata cccgttcctg ctcctgtatt cggggaatta ttacaggaaa tacaagatat
    17401 aactgagcta aaaataattc tccgaattat ccgtctatta cacaataaaa aagggttaga
    17461 gagatatgta tccgtagacg aattacttgc agatcgtgtt ctttcaatcg gcctgggctc
    17521 gatggatctg aataaccgga aaacaaatat aattcagtct ttggaatctt caacaaaacg
    17581 aaaaatatta attaaaatca acaggaaaga tataggaaca tctgcatact tcctgaacac
    17641 ggaatttgaa agacaatcaa tgaaaaaaat gccggatttg gaacaccaat ccgactttga
    17701 gccctgggaa acgggggaag atagaccaaa tatatattct ctttacgaac agaatattgg
    17761 tatattaact ccgattgtgg cagaaaaaat cggggaagcg gaacaaaaat acccagttga
    17821 atggatagag gaagcgatag cagaagcagt atctctcaat tcaagaaatt ggagatatat
    17881 ttcaagaata ctagaacggt gggaaataga gggaaaacaa aatggagaat ctaggaagca
    17941 ttctggcaaa gctagataca tttaaagcca acagaagcaa cgacctctct aaagatatag
    18001 atctagatcc acctataaat tgcgatatat gtaatgacaa aggatggctt acacccaaac
    18061 ttccaactgg ccaccaagac ttcggatcca taaaatcttg tgcttgccga aaaaatgtaa
    18121 aacaggaaga aatgacgact aggctacttt cctatagtaa cttaggatcc ctctcacgct
    18181 acacctttga aaccttagat ctgaaaaatg agccaactca acaaaatgga ggtgacgtct
    18241 ttcgtgaggc tttcgtaaac tgctccaatt attcagaaca tccgagcggc tggatattga
    18301 tcaatggccc acatggttcc ggcaaaaccc acctttctgc agcgatagcc aacagatgta
    18361 tttcgaccgg caaaccggca ttctttatat atgtgcctga tttaatggat catctcagag
    18421 ctagctattc aaataattta gactttgaat acgatgattt atttgaccag gtaaaaaatg
    18481 ctccaatatt aattttggac ggcataactg accacagcgt aagtagttgg tcggccgaga
    18541 aattgaatca gatcctcaat catcgatcaa acgggagact ccccaccgtt ttgaccacgt
    18601 ctgaagaaat cgattcctta gaccctttta tcaaaagcag aatatacgat tcaaatcttt
    18661 gcgatatagt cacaacggat tcaagaatta gtaattatga cgaaatatct tccctaggag
    18721 ccattcctaa agaactttca cagatgaact tcgatacctt taacccaaaa ggaaggaggg
    18781 gaaattccaa tttagaccca gtgtatgaaa gtgccaagaa atacagtcag accccagatg
    18841 gatggctcac cttctatagt gataactcag gagtgggtaa aacccattta gcagttgcta
    18901 taggaaacta tcggaaatca attggcgagg atgtcttctt tgcattcgta ccagaattga
    18961 tggatgaatt gagatctgga ttctctccag acagttcact taattcatcc cgtatatttg
    19021 acagggttaa ggattcctca ttattgattt tagatgactt gggcaatgaa caagacacag
    19081 attggtctca aaaaacaatt taccatcttt tggtttaccg acataatcac aggcttccaa
    19141 cgattataac cacgaggaca gatttcaccg acatagctgg tagaagtgca caagcttcca
    19201 gaattcaaga cgccaaaata ggccaaattc taaacataca agctccagat tacagaatgg
    19261 gtagaactcg atagacacag gaaccacact tttaaatgac aatagatcaa gactgtattt
    19321 tctgcaaaat catttccggg caggaacctg caactttcca ccatgaggaa aaagattttg
    19381 tggtatttga gaataatctt aaatggtttt caactcaatt cctttttgtt cccagattac
    19441 atatgaacca atctaattta tggtcgtcaa aaaacttgat atctgacatc ggatttcttg
    19501 ccaacgagat aggtcagaaa agatgcccat ctgggtatag gattctatcc aattttggag
    19561 tggacggtat gcaatcccag gagcatgcac acctgcacct agttggtggg agtaaattgg
    19621 gaatgtacgt atggggtaag ctgagaaaaa atccactata acgaggtata gaaaatcacc
    19681 ggaacatctg ttcacacacc aaatgtgaaa ttgcagttat acccctaacc cgtataaatt
    19741 cgtcttcacc gctacgagct aagtgcgtta tcagcccata gcattatttc ttcggtttta
    19801 tcccaaccaa cacaagcatc tgtgatcgct accccatatt ccaatgtacc tgggtcatta
    19861 ccgaatttct ggcttcccga atttatattg ctttcaagca tcactcccac aatactattg
    19921 ttcccgcctg ctcgttgtgc aataacatct tcaaacaccg aaggctgtct ttcatgatcc
    19981 ttattggaat taccgtggct gcaatcaatt actaaccgtt ggtccaaatc tccattggat
    20041 aaaacttcac tggccaactt tattgattct gaatcatagt tgggacctaa tttccccccc
    20101 ctcaacacca gatgaccatc tggattgcca tcagttctta aaacacaaac gtgcccatca
    20161 ggtccagtac caacatatga atgctggttc cgagctgcca atataccatc tactgcagtc
    20221 tcaaaacttc cgtcggtacc attcttgaac ccaaccggaa taccgatagc gctcgccatt
    20281 tgtctatgag tttgactctc agtcgtcctt gcacctattg cagcccaaga gactaaatct
    20341 tcaaaataat gcgggactac aggatccaaa aattcacaag ctgcgggcat tcccatgtca
    20401 ttaatttcct gcagcaatct ccttgctcgt tgtaatcctt gtgagatatc aaatgtctcg
    20461 tcaagtccag ggtcatttat caggcccttc caacctaccg ttgttctggg cttctcaaaa
    20521 tatactctca taacaacaaa tattttctta ctaaggcggt ttctcagctc gattagtctt
    20581 cgagcataat ccagagcagc ctcctcatca tgaatagaac aaggccccgt tattatcatc
    20641 actctgtcgt catccccatg gaggatgtcc tttattgttc ttctagcatt ttgaacggtt
    20701 gaagccgcct catcggatct agggaacctg tccactagct cgagagggct aaacaaatga
    20761 ttagaagaaa atcccaatcc ttggacatga cttttttgac tgttcatttt tatccttaat
    20821 ttttgaattg aatatttttg taatatcttt tgactttatc aataaatcat aaagcaaaaa
    20881 aaaaagcccc aatcgctacc gattgtggcc ttctgtctga ctttggatat attcctagag
    20941 tttacacaag tcctttatcg gcagctgact taagctacta aaataatagt aagacatgtg
    21001 tgaattatca tagagtttat tttccatagc acggcggata gtatactctt tcacaaacag
    21061 tgtcaacaaa tctccaaccc aagctaagaa gcacaaatat gtctgatata cattctgaaa
    21121 tgagcccctg ttgcacggct tcaagggaat ccaaactgcc ctttcatccc tccaaaatta
    21181 aaaacacacc gaaatctcag acgaaaaata ttaatccgga aaacatggcc actattccag
    21241 gtgggacatt tcaaatgggc accgattacg aattcagctt cccgggtgat agggagggac
    21301 cgataaaaaa agtgagtctg aattctttcc taattgatat tacagctgtt acaaataaga
    21361 attttgctga tttcgcaaaa gaaacaaaat ataaaaccga cgctgaaaaa tttggctggt
    21421 catttgtgtt ttaccagttt atttcgccta aaaatgcggg ttcggccaac cagtctcccc
    21481 caggtacccc gtggtggaga aaagttgacg gggcgtcttg gaagcaccca gaaggttcag
    21541 gttccaatat aaaaactaga atgaaccacc ctgcagtcca tatctcatgg aacgacgcaa
    21601 ctgaatttgc atcccattat ggaaaacgtc tgccaactga agctgaatgg gaatttgcag
    21661 caaggggcgg ccaagaacaa cggttatacc cctggggaga cgagctccat ccgggaggcc
    21721 agcacatgtg caatatatgg caaggagaat tcccaaccac aaactcgaaa gatgatggat
    21781 tcacagggac tgctccagcc aaacgttacc ctccaaacgg ctacggtttg tataacgtag
    21841 tcgggaacgt ttgggaatgg cagcacgatt ggttcagtaa ctcactcaac gatatagatg
    21901 ccaaacataa ccctaagggc cctaatt
//
LOCUS       DASCNS010000038         2807 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000331132, whole genome shotgun sequence.
ACCESSION   DASCNS010000038 DASCNS010000000
VERSION     DASCNS010000038.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000331132"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="QF838_01470"
     CDS             complement(<1..620)
                     /locus_tag="QF838_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO59394.1"
                     /translation="MKITKINTYHASDGQRNNIFVEVETDEGITGTGEAYSIGPDKSV
                     ISMLEEMEPWFVGQDPSRIEWLIRRAKNTMRFPLGQVAWSAISSIDIALWDIAGKAAG
                     VPVYVLLGGKTRDKVRVYHAIYGDSPQLLAENAQHLIEEGYSAFKTSPVPSNWRKLPW
                     REVLQMADERMRTIRDAIGNAPDLAVDVHVAIRETSQALELARVITP"
     gene            685..1761
                     /locus_tag="QF838_01475"
     CDS             685..1761
                     /locus_tag="QF838_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014615.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HJO59395.1"
                     /translation="MENNSTSPEETIKMAVYKDLKARLDRGDTIIHDAAVSTELQSMG
                     VPMDFVAWSGPTNYTHPSSVVQMHERHIRAGSDIITTNTWSTLRPTLERAGYGDFVRE
                     INSRAVHLAQKARERASNGRDIYIAGSLSSHITGRDPRTGEIGFGAFSSSPQIPVAEL
                     EQYYGEVTGIMAEAGVDFFIVEAMGRDNEARIIQARAAKETGLPVWIGFNAYIDSKDN
                     VMAGGQQNPTDIAEGTPLEGSRTMKDFTLREAVNEAISVGPDVISIFHTRIANANRAV
                     KVVLEEWSGPVMVYPDAGRQSASLAKWHNHSIENEESVEDYIDAAKAWVDQGVQIVGA
                     CCGFGVQYIEPLRQSVPEKISIPR"
     gene            1793..>2807
                     /locus_tag="QF838_01480"
     CDS             1793..>2807
                     /locus_tag="QF838_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59396.1"
                     /translation="MTRKSINPDIGYELANPTGVSISPDGNLAVYGVSKPDTKSGKLN
                     NSLWIKDIKDSGSAPRKLTSGTTDENPKFSSDASLIAFLRDDSKGKKQIWVLQMSGGE
                     ASMVTSLPESIRDFSWKPNSTGFAAVADVDPDYDGDTDYDEIQVIPVSRIRYRHDELR
                     WRGNSVTQLFDVDIATKESKQLTFDDGDQGIPSWSPDGLKLAFISDAMPERDFHAGNE
                     VKVLDLSSGKIDCWSKGLADACSANWLPDSNSLAVIGSEDFRLHSYYQGSVYILSPGS
                     DPVRVTDDNVAPIGGSHPAKNGPPLFIDENGVILFAGQSGAESHIYEARIGACGQNKV
                     VDLG"
BASE COUNT          786 a          632 c          678 g          711 t
ORIGIN      
        1 ggcgttatta cccttgcaag ttccaaggcc tgtgaagttt ctcttatagc cacgtggaca
       61 tcgaccgcga ggtcaggagc attgccgatt gcatctctga ttgttctcat tctttcatcg
      121 gccatctgaa gaacttcccg ccacggtaac tttctccagt tgcttggtac agggctggtt
      181 ttaaaagcag aatatccttc ttcaataagg tgctgcgcat tttcagcgag tagctgcggg
      241 gaatctccat atattgcatg gtaaacacga actttgtctc tggtcttacc cccgagcaaa
      301 acatagacag ggacccctgc cgccttcccc gcgatgtccc agagagcgat gtcgattgat
      361 gaaatggctg accaggcgac ctgtcctaga ggaaagcgca ttgtgttttt tgcacgtctt
      421 atcagccatt caatgcgaga agggtcctga ccaacaaacc atggctccat ttcttcaagc
      481 atcgatataa cacttttgtc gggaccaatt gagtaggctt caccagtgcc cgtgatacct
      541 tcatccgtct ccacttcgac aaaaatattg ttgcgctgac cgtcgcttgc gtgatacgtg
      601 ttgatttttg taattttcat catctgaatc caaaacttgt gtgtttttag agtatgccat
      661 atatcacccc gataggtatt atgtatggaa aataattcca ccagtcctga ggagacaata
      721 aaaatggcag tctataagga tcttaaggcc cgactagacc gaggggacac gataattcac
      781 gatgcggcgg taagtactga acttcaatcc atgggggtac ctatggattt tgtggcatgg
      841 agtggcccta caaattacac tcatccctca agtgtcgttc agatgcatga acgacatatt
      901 agggccggat cagacattat caccacaaat acctggtcta ccctgcgtcc aacccttgaa
      961 cgcgctggtt acggggactt tgtgagagag ataaatagcc gtgctgttca cctggctcag
     1021 aaagcaagag aaagagcctc caacggtcgg gacatataca ttgccggttc attatccagc
     1081 catataacag gaagagatcc tcgaacaggt gaaattggat ttggggcatt ttcatcgagt
     1141 cctcagatac cggttgcaga attagagcaa tactacggcg aggtcaccgg tatcatggca
     1201 gaagcaggtg tggatttctt catcgtggaa gccatgggac gtgataatga ggccagaatt
     1261 attcaggcaa gagctgcaaa agaaactggc ctaccagttt ggatcggctt caatgcgtat
     1321 attgattcaa aggataatgt tatggcggga gggcagcaaa accccacaga tatcgcagaa
     1381 ggcactcctc ttgagggatc gcgaaccatg aaagatttca cgctgcgtga agctgtaaat
     1441 gaagctattt cggtcggccc agatgtgatc tccatattcc ataccagaat cgccaatgcc
     1501 aacagggcag taaaagtggt tttagaggaa tggtcaggcc ctgtgatggt atatcctgat
     1561 gcaggacggc aatcggcctc cttagctaaa tggcataacc attccataga aaatgaagag
     1621 tcagtggaag actacatcga tgctgcgaaa gcatgggttg atcagggggt tcaaatagtg
     1681 ggagcatgct gtgggtttgg ggtccaatac atagaaccct tgcgccagag tgttccagaa
     1741 aaaatatcta ttccacggta aatatactga caaacacttc agagagggca aaatgacgcg
     1801 aaaatcaata aatccggata tcggttatga actagcaaac cctaccggag tatccatttc
     1861 gcctgatgga aacctagctg tttacggagt ttccaaaccg gacaccaagt caggaaagtt
     1921 aaataattcc ctttggatca aagatataaa ggattcaggt tccgcaccta ggaaattaac
     1981 gagcggtact accgacgaga atcccaaatt ctcatcagac gcctcattaa ttgccttttt
     2041 aagagatgat tcaaaaggaa aaaagcagat ctgggtattg cagatgtctg ggggtgaagc
     2101 tagcatggtc acttcgctgc cagaaagcat tagggatttc tcgtggaagc ctaacagtac
     2161 aggatttgcc gcggtcgcag atgtcgatcc tgattatgac ggggataccg actatgatga
     2221 aattcaggta attcctgtat caaggataag atacaggcac gatgaactgc gctggcgcgg
     2281 taacagtgtt acccaactct ttgacgttga tatagctacc aaagaatcaa aacaactcac
     2341 ttttgatgac ggagatcaag gaataccctc atggtcaccc gatggtttga agctggcctt
     2401 catttcagac gcgatgcctg agcgagattt tcatgccggg aacgaagtaa aggtattaga
     2461 tttatcctca ggcaaaattg attgctggtc taaaggacta gctgatgcgt gctcagctaa
     2521 ctggctccca gacagcaatt cacttgcagt tattgggtct gaggattttc gacttcacag
     2581 ttattaccaa ggctccgtct acatactctc acctggctca gatcctgtca gggttaccga
     2641 cgataacgtc gctcctattg gtgggagcca tcctgcaaaa aatgggcctc ccctatttat
     2701 cgatgaaaat ggagttattt tatttgcagg ccaatctggc gctgaatcac acatttatga
     2761 ggcaagaatc ggcgcatgcg gccagaataa agtagtcgat ctaggag
//
LOCUS       DASCNS010000039        12394 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000343497, whole genome shotgun sequence.
ACCESSION   DASCNS010000039 DASCNS010000000
VERSION     DASCNS010000039.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12394
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000343497"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(111..983)
                     /locus_tag="QF838_01485"
     CDS             complement(111..983)
                     /locus_tag="QF838_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HJO59397.1"
                     /translation="MGGGDTNLLGSKVNSGGAEEARTPDPLLANRITLDSYLNSLKVR
                     NCSDKYIGANKRFLLTLVNTFPIINEESALQYLGTFNDIGANSRARYGGMLKGLLNHM
                     GMSFDTKFKRPKLLPQRVLHEDVEKLKDTIRNKKSHKKSAFRDLILIETAIKTGMRRG
                     ELANLLVSHIAFEANRIIVMNGKGAKDRTIPMGDNLKSQLLELCAGKKTNEKVFGFKA
                     VSLGMKIKEWGDKAGVPIKAHSLRHYFATNLMERGANIKQVQELLGHESLATTQIYLS
                     LMADHLEDAIQLLE"
     gene            1042..1389
                     /locus_tag="QF838_01490"
     CDS             1042..1389
                     /locus_tag="QF838_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59398.1"
                     /translation="MKKMLFIALVLLVKVGCSETEVDTPSFADGQATALTIEAICDGL
                     GYDFAMQEGSPLPLSEFTEEYQGLGVWLVQLVIKNDYKWNVYESTGTVTTAGTYETYM
                     KYGTSRTATRHIC"
     gene            1465..2247
                     /locus_tag="QF838_01495"
     CDS             1465..2247
                     /locus_tag="QF838_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59399.1"
                     /translation="MKKMLLIALVLLVMVGCADTVAAPSEQKLLAVPTAYTAQMPQPN
                     PAQSTSQGDKPGDKYTCAINSSGYCIFSGRPHKTGLKPYTEDIVDADGVFLFTMNEAV
                     AVNSSGEILQAKGTPASNGDELVRYSKGEMTDDEKAFHRVMAFMFPIRNALMYDISEV
                     SQARWEELVSELKMRGVKDKTFTDGPTPKDNYYGRNGIFELAKNPEGKDIHHDVMKFL
                     EEAGLYFLCHVTSDGFGQMLQDTHPEGHNPCEDAKITEKIPF"
     gene            2350..2574
                     /locus_tag="QF838_01500"
     CDS             2350..2574
                     /locus_tag="QF838_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59400.1"
                     /translation="MLREKSLEWFGVLTAIAYSMLVASNTGNEVLGFTLLFISAIAIG
                     VWSFSFKHYGMLFLQIFYAAAGVIGVMRWL"
     gene            2830..3324
                     /locus_tag="QF838_01505"
     CDS             2830..3324
                     /locus_tag="QF838_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJO59401.1"
                     /translation="MQNQNDLLIIYNSKNGRTERMVEPIHQGIRESGLEATVRLVSDV
                     RWEEMLSAKGIIVGTPVRFGGIDWEIKRLFDVTAHQDYPGPLSGKVGGAFTAGASPGS
                     GAELALLSMLHVLLNHGMVIQGNAFSSHYGPIALSESSEDSVVSKCYEWGQHWGRLVR
                     KLHD"
     gene            3449..3643
                     /locus_tag="QF838_01510"
     CDS             3449..3643
                     /locus_tag="QF838_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59402.1"
                     /translation="MKKIVLIALVILVMVGCAKTAQIVPIPDTVNDFSLTDTLNNKVQ
                     LTDLMNTNDGVVIVFFRGHF"
     gene            3647..3982
                     /locus_tag="QF838_01515"
     CDS             3647..3982
                     /locus_tag="QF838_01515"
                     /inference="COORDINATES: protein motif:HMM:NF020123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HJO59403.1"
                     /translation="MCRQQLVELEGVYNEFKKYNAELVAISMDSRGDTLNLIRELGLS
                     YYVLPDPTGKVVKKYDVYNLLGDGVATPSVFIVSSEKSINWKYIGESISDRPDIETLL
                     SEIRKTSGK"
     gene            complement(4139..5035)
                     /locus_tag="QF838_01520"
     CDS             complement(4139..5035)
                     /locus_tag="QF838_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HJO59404.1"
                     /translation="MRGKIVIGSAAKRIYMKAFVITFVVLLSSFFISCSDDGISVSSG
                     TEVARAMSSDYPMSSPAYAPSPEIESYSKGSSEPRIQINGNLSLEVQNVPAALEQARE
                     LVIQYGGSITSSDSGGSLNRYANISALIPRDSFYELIGVMKNIATKVSNENINSNDVT
                     EEFVDTEAKLNVMKETEKRFITLLSETANVEEVMSVERELMRLRGEIDSLEGRMKYLG
                     KTTDNSVLNIYMTEEMPITGSAWSFFDSLDNSVRDMVSFSKHIANFLIGIIVFSPILI
                     GVILIVFLVYKYGKRYVLPRKK"
     gene            5259..5894
                     /locus_tag="QF838_01525"
     CDS             5259..5894
                     /locus_tag="QF838_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59405.1"
                     /translation="MNTRVLTSWLMIICPIGMMVMFAGLEPLIMGAAGEGLGPNERVL
                     AGLETFNDKKVAGYFISIFGLLFMVGMISALALLGKSLKGAGAAIGTLSSLIFTAILT
                     ITVVGMGLSFSANQIFADGYKDVAVSLELISDSAFMGMPVFWGLGLALLGLGMLLEKR
                     PLPTVLSGLLCLIGIIMFPAALILDDIGFAVFAISIVLVASSGGVLLRQQD"
     gene            6107..6475
                     /locus_tag="QF838_01530"
     CDS             6107..6475
                     /locus_tag="QF838_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59406.1"
                     /translation="MNWVTAIKNWMLFGLTGVVSTLVFKLAHDLFTSDSNFSLWEFAI
                     SLIIAGSASLLISKLSHSKSGFFLVVTYMTLLIPVLGALFGSSGSEPLWQFGLLGLFG
                     SLFWSVPFSIWAGWKYGKVQ"
     gene            6609..6914
                     /locus_tag="QF838_01535"
     CDS             6609..6914
                     /locus_tag="QF838_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HJO59407.1"
                     /translation="MTVKVLAEFNCVDGNADEFIEICQNIFPVTREFDGCSHIDISAD
                     QEDPNRLIMTENWASKEHHLKYLEYRTDDGNLEKLVALLESPRRSPTSIYRMHGLIR"
     gene            6949..7887
                     /locus_tag="QF838_01540"
     CDS             6949..7887
                     /locus_tag="QF838_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HJO59408.1"
                     /translation="MIHSFEPDKYYLSIGSHPPALKINSGDTVNTNTVDAGGFDKKGI
                     LVSGRGNPQTGPIYVEGACPGDVLAVRLNNIWPNRGHGFCRPKIASNVLEPGHDSRVT
                     DLDTFDFLLDFDSNTARIENPSEGLTDLSVPIRPMIGCFGVAPDRGQAISTATSGPHG
                     GNMDYRGFESGVTAYFPIFVDGALFHIGDGHAWQSDGEILGTGIEISMDVSFTLTVIP
                     KIEIFWPRGQNEDFIFTAGNVRPLDEALQHSTSEMLRWLVNDYALSWPDANIMLGTYV
                     QYDVGNVFDPAYTMICKIPKSVLPSASPGSLISQSA"
     gene            complement(7897..8763)
                     /locus_tag="QF838_01545"
     CDS             complement(7897..8763)
                     /locus_tag="QF838_01545"
                     /inference="COORDINATES: protein motif:HMM:NF023144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HJO59409.1"
                     /translation="MLTLKDPNTVLAIVAESSNKFITGLADLKPTEWEIPSKCHQWAV
                     KDVSSHTLAIDGFLLNSLSRAIEGDGNPTEGMPNPGTGNARSMADGIASRAIQISETA
                     FPKLEQLLDTLSNMEWDLINAFQNIASENWHIPAYHPAGKFSPYLLLKMKLLELVIHS
                     WDIFSSLNSSYKMQNSEAKLLCEVWKEPNISNWLYTPDLEQIDPTTIDFCFDDSEALR
                     IQTWRGSLHIFDRPIEKATETITTVETTYQDFALLITARRNIHEAYNSNVISVTGDKA
                     KLDSFHKWFRGS"
     gene            complement(8757..9635)
                     /locus_tag="QF838_01550"
     CDS             complement(8757..9635)
                     /locus_tag="QF838_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59410.1"
                     /translation="MLYHDPIFSVKDKTAVITGASSGLGVTFAQILAERGANVVLSAR
                     RIENLNTLKESFDSENFSSMVAQCDVTESTQVANMFTKAEEKFGRVDILVNNAGQIAE
                     AGSVPENVTDYMFEQTVKVNLIGLWYCCKEGGRRMLSDGRGGSIINLSSVAGLNGMRG
                     MPVAYQATKGAVTTLTKSLAANWADRGVRVNAIAPGWFPSEMTGAWVKKGMFLDHISA
                     NSPMGRIGKPEELTGALLLLASNASSFINGEVISVDGGVNASTGLPFFTEEMFSVMEK
                     AVPGDLGKRVKPPEKC"
     gene            9850..10452
                     /locus_tag="QF838_01555"
     CDS             9850..10452
                     /locus_tag="QF838_01555"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HJO59411.1"
                     /translation="MYDTLVGTFIGFLSGSIPWSLICVRTLSGRDVRKVGDGNPGATN
                     AWISSGWMIGSISMLLDVLKAAVPVWVVTNQFTGTSYSEDFLSVTVVALSPIIGHAYS
                     PLLRFRGGKALAPSWGSWIALTSGMAFPVAVLILGVMHLMQRNHAITVTTCLIGFTVI
                     FSIIDSQALIIFGILNLMLVTFKHRSEYQQGIVFRGRERT"
     gene            10458..11624
                     /locus_tag="QF838_01560"
     CDS             10458..11624
                     /locus_tag="QF838_01560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJO59412.1"
                     /translation="MELSTLITFTSGFFAFTSFLITIWNWQYLDRLRLCPSNELFGDT
                     DVSITVLIPARNEGKNIGEVLTALSCQTHINYDVIVLNDGSEDDTAEIVSQFLASDLE
                     IQLIDGEPIPKGWLGKNWACHQLMLKADGNYILFIDADTVLHEDTIKAALNEAILEKS
                     DLLTMIPARCANNFIERNLYGFIDWAIYAWLPLQLAHLSKNSYLSASYGQFMLFKRES
                     YILSGGHRKIKDIAVDDFGLGRLIKSSGLKWALKDGTNMVTSLPYDDILETVNGVSRS
                     LAPALNYRISLILLISIFLMGLFFAPVLNLYFYVANGGQFEFFELLSLVTIFLLLGSY
                     LLSCKRFSHSLFIVVFIHITVIFMILIAYHSLLSNLLRVATWKDRNMVIKKLKI"
     gene            11887..>12394
                     /locus_tag="QF838_01565"
     CDS             11887..>12394
                     /locus_tag="QF838_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJO59413.1"
                     /translation="MAINGLAHAPDVLKRVRKEKGLTQKAVAALVGVNQSVISRIEAG
                     EIGASNRIAEGLEEAYGMPIIDALLYRKSWTGEPEDVTEIKQLIDRYQTVLSDSGIKA
                     IKAYVEHMIGLSNGPDFLPGGSAIPIKSLWGGDWTWGPRELERHITVECAPVMTWNRI
                     SIHRSHPIT"
BASE COUNT         3422 a         2391 c         2745 g         3836 t
ORIGIN      
        1 agttgaactg aatgttgaga agtttctaac cacttttttg aatttcggta agcccatttt
       61 ttgtgccatt cgaagaccta gatgaagact gtggccgtgg atggcgctcc tcattctaat
      121 aattgaatag catcctccag atggtcagcc attaaggata gatatatctg agtggtagcc
      181 aaactctcat gtcccagtag ttcttgtact tgcttaatat ttgcccctcg ctccattaga
      241 ttggtagcaa agtaatgcct caaactatgg gccttaatcg gcactccagc cttatcaccc
      301 cactccttaa tcttcattcc aagacttact gccttgaacc caaatacctt ctcatttgtc
      361 ttcttaccag cacatagttc cagcagttgg gacttgaggt tatcgcccat tggtatggtt
      421 cggtctttag cccctttgcc gttcattacg atgattctat ttgcctcaaa tgcgatatgg
      481 ctcactagaa ggtttgccaa ctctccacgc ctcataccgg tcttgatggc agtctcaatt
      541 agtattaggt ctctaaaggc tgatttcttg tgggacttct tatttcttat agtgtccttc
      601 agcttttcaa catcctcgtg tagtactcgc tgggggagga gtttggggcg cttgaacttg
      661 gtatcaaagg acatgcccat gtgattgagt aatcctttta acatgccccc atatctggct
      721 ctagagttcg ccccaatgtc gttgaaagta ccaagatatt gaagggctga ttcttcgtta
      781 attattggaa aagtgttaac aagtgttaac aagaatcttt tgttggctcc tatatacttg
      841 tctgaacaat tacgaacttt gagggaattt aggtacgaat ctagtgttat ccgatttgca
      901 agcagggggt caggagttcg agcctcctca gctccaccac tattaacttt agaacctaaa
      961 agattcgtat caccaccccc caacccttct actgttttgt tctttttgtc accattatag
     1021 gcatcttggt attatgtgcc catgaaaaag atgctattca tagcgttggt tcttttggtg
     1081 aaggttggct gttcagaaac tgaggtggac accccttcat ttgctgatgg tcaggcgact
     1141 gcactaacaa tagaggccat atgtgatggc ttaggttatg actttgcaat gcaagaaggt
     1201 tcccctcttc ctctgagtga atttactgaa gaatatcaag gtcttggagt atggttagtc
     1261 caactggtca ttaaaaatga ctacaaatgg aatgtttatg agtccacggg tactgtaaca
     1321 actgccggca cttatgaaac ttatatgaaa tatggaacct caagaactgc tacaagacat
     1381 atctgttagc taattcagtc tgtgttgtac tatgctcgcc ggatagagat ggctagggga
     1441 accccctttt ggtattatgt gcctatgaaa aagatgctac tcatagcatt ggttcttctg
     1501 gtgatggttg gttgtgcaga tacggttgct gctccttcag agcaaaaact actggccgtt
     1561 ccaacggctt acacagctca aatgccgcag ccaaatcctg cacaatcaac cagccaggga
     1621 gataagcctg gagataaata tacgtgtgca ataaactcca gcggttattg tattttctca
     1681 gggagacctc ataaaactgg tcttaagcca tatactgaag acatcgtaga tgctgatgga
     1741 gttttccttt ttaccatgaa tgaggctgtg gcagtaaact cctctgggga aattctacag
     1801 gccaaaggaa ctcccgcttc taacggagat gagctcgtga ggtattcaaa aggcgaaatg
     1861 acagatgatg aaaaagcttt tcatagagtg atggccttca tgtttcccat tcgtaacgct
     1921 ctgatgtacg acatttcaga ggtaagccag gctaggtggg aagagctagt ttcagaactc
     1981 aaaatgagag gagttaaaga caaaacattt acagatggcc ctacccccaa agacaactat
     2041 tacggaagga acggtatttt tgaattagct aagaacccag aaggaaaaga tatccaccat
     2101 gatgtaatga aattccttga agaagcaggg ttgtattttc tatgtcacgt taccagcgat
     2161 ggctttgggc aaatgcttca ggatacccac ccagaagggc ataacccttg cgaagacgcg
     2221 aaaataacag agaaaatacc tttctaataa tggggctacg tgcgaattat catccctcct
     2281 accatagtgg gtaagaggca ggggtttacc tattctgtaa tcttgaaaat agtttcgtaa
     2341 ggatgaccaa tgttaagaga aaagtcactg gaatggttcg gagtattgac agctatcgca
     2401 tattcaatgc ttgttgcttc caacactggt aatgaagttc ttggctttac acttttgttt
     2461 atttctgcaa ttgcaattgg agtatggtct ttttcattca agcattacgg catgttgttt
     2521 cttcaaattt tttatgccgc agctggcgtg ataggagtca tgaggtggct ataaagatac
     2581 caacttcatg tgccggcggc gccgccggga cgctccccat catgattatg gctgtaatgc
     2641 ctctgttaat tctcgtatta gttcggtaaa ctcgaccacc cgttcttcag gggaagatat
     2701 tctttcctct gctgtttcac ctgccatacg ctttcaagaa tacagaatac agaatacaga
     2761 atacaagaca agtcgtggtg caattacgtc atactataag aaataaagaa atgtcagaag
     2821 gtactcaaga tgcagaatca gaatgattta ctgataattt ataattccaa aaacgggcgt
     2881 acggaacgta tggtggaacc tattcaccaa gggattcggg aatccgggct agaggcaacc
     2941 gttaggttgg ttagcgacgt tcggtgggaa gaaatgttgt cagctaaagg aataattgtc
     3001 gggaccccag ttaggtttgg aggtatcgat tgggaaatca agcggctttt cgacgtgacg
     3061 gcccatcaag attatccagg cccattgtct ggaaaggtcg gtggtgcatt cactgctgga
     3121 gcaagccctg ggagtggtgc agagttagct ttacttagca tgcttcatgt attgctcaat
     3181 cacggtatgg tcattcaggg aaacgccttt tcgtctcatt acggccctat agcactatct
     3241 gaatcttccg aggattcagt tgtttcaaaa tgctatgagt ggggccaaca ttggggtaga
     3301 ttggttcgca aactccacga ttgaagttat aaataattaa tcaaaaatct gtgtgggact
     3361 atgctcactg gatagagatg gctggggacg aagcccctta ttacgtgcga gttattgcga
     3421 caatatgtca tcttggtatt atgtgcccat gaaaaagata gtactcatag cgttggttat
     3481 tttggtgatg gttgggtgtg ccaaaactgc tcagattgtt cctatacctg acacagtcaa
     3541 tgatttttct ttaacagaca ctctcaacaa taaagttcaa ctcactgatt taatgaacac
     3601 caatgatggt gtagtcattg ttttttttag aggtcatttt tgaggtatct gcaggcaaca
     3661 gttggttgag cttgaagggg tttataatga attcaaaaaa tataatgccg agctagttgc
     3721 cattagtatg gacagcagag gagacacttt gaacctaatt agagagttag ggctaagtta
     3781 ctatgtcctc cccgacccga ccggtaaggt ggtaaaaaaa tatgatgttt acaatcttct
     3841 tggagacggg gtggctactc catcagtatt cattgtgtcc agtgaaaaat caataaactg
     3901 gaaatatatt ggggaaagca ttagtgatag gcctgatatt gagacacttt tgtccgaaat
     3961 tagaaagaca tcgggaaaat agttctaata atgcacaatc tctggaatag tattcaaaaa
     4021 tctgtgtcgg ggtatgctcg ctggatggaa aggcgaggga cgaagccccg gtacgtgcgt
     4081 atggctccct ttaggcacta cacatctgtc agcagtagat acccgtccgg atacgacact
     4141 attttttgcg agggagcacg tatctttttc cgtacttgta gactagaaat acaatgagta
     4201 ttaccccaat taagatgggt gaaaagacta ttatccctat caagaaattt gcgatatgtt
     4261 tcgaaaaaga aaccatatcc cttactgaat tgtctagaga atcaaagaaa ctccaggcac
     4321 tacctgtaat cggcatttct tctgtcatat agatattcaa aacggagtta tctgtagttt
     4381 taccaagata tttcatcctc ccttcgaggc tatctatctc gcccctgagt ctcatcagtt
     4441 ctctctctac actcattact tcttccacat tggctgtttc ggagagaagg gttatgaaac
     4501 gtttctctgt ttccttcatc acattcaatt tcgcttcagt atcaacaaat tcttcggtta
     4561 catcattgga attaatattt tcattggaaa ctttcgtagc gatattcttc atgaccccaa
     4621 ttaattcata gaatgaatct ctcggaataa gagctgatat gttggcgtaa cggttcaagg
     4681 agcctcctga atctgacgat gtgatactac cgccatactg aatgaccagt tccctagctt
     4741 gttctaaagc ggcaggtacg ttttgaactt ctaaagacaa attcccgttt atttggattc
     4801 taggttctga cgaacctttg gaataacttt caatctcggg tgagggagca taggcaggac
     4861 tgctcattgg gtagtcagaa gacatagcgc gagccacttc tgtgccagac gataccgaaa
     4921 taccgtcatc tgaacacgat atgaaaaaac tcgataacaa aactacaaaa gtgataacaa
     4981 aagctttcat gtaaatccgt tttgccgctg acccaataac tatttttcct ctcatcacat
     5041 tgtacgtcac tcctcctaaa aaagagtatt tttacgtagt atgagtggtt actcttacaa
     5101 ttgaatagga ttcttcagat agtcagatgt tatcggttgg cttacgggat gttctatata
     5161 ttttggtgga tctttagtga tttttttatg gcttttttga ttttatctaa ttatcttaga
     5221 ttcagaattt ggtaacaggt gagtggtagg agtagcacgt gaatactaga gttttaactt
     5281 catggttaat gataatctgc ccgataggga tgatggtaat gtttgccggc ttggaacctc
     5341 tgattatggg cgcagccgga gaaggtttag gtcctaacga aagagtgtta gcaggtttgg
     5401 aaactttcaa tgataagaag gtggctggct atttcataag tatttttggg ctattattca
     5461 tggttggtat gatttcagca cttgctctcc ttgggaaatc tttgaaaggg gctggagctg
     5521 cgatagggac tttatcctcg ttgattttca cagcgatatt aacaattact gttgtaggta
     5581 tgggattatc ctttagcgcc aatcaaattt tcgctgatgg gtataaagat gttgcagtgt
     5641 cattggaact tatatccgac tctgccttta tgggaatgcc tgttttctgg ggactgggtt
     5701 tagcactatt agggttggga atgctattag aaaaaagacc tctgccgacc gtattatctg
     5761 gtctcctgtg cttgattggt atcataatgt ttccagcagc acttattctt gacgatatag
     5821 ggttcgcggt attcgcaatc tccatcgtgt tagtggcatc ttctggtggg gttctcctta
     5881 gacaacaaga ttgacacatg gttttagagt tgaaaggaac cccattgtag attaatcaaa
     5941 catatgtgtg ctgataggcc tctgaatgag tagctggaaa gaggaaaccc atttgaaccg
     6001 tgaacctgct gattaagagt cagtaactaa ggagaggtag tttgcggatt actgctcatt
     6061 atcaagactt gtatagaagc ctctatctgc aatcatagaa gatgatatga attgggtcac
     6121 agcaataaaa aattggatgc tttttggcct tactggagtt gtaagtacac tagtattcaa
     6181 acttgcccac gatttgttta cttccgattc taatttcagt ctgtgggaat ttgcgatttc
     6241 tttgattatt gccggatccg cttctttgtt gatatccaaa ttaagccact ctaaaagtgg
     6301 tttttttttg gtagtgactt acatgacatt acttattcca gtgttaggag cacttttcgg
     6361 gtcctcgggt tcagaaccat tatggcaatt tggattgttg ggtttatttg gatctttgtt
     6421 ctggagtgtc ccgttttcta tttgggcggg ttggaaatac ggaaaggtcc agtagctgga
     6481 aggtaatcga tcacctagga aacctcatag catatatttt actcccaatt gtgaacatat
     6541 ttccttttgg gttatcgact ataatgaccg agctaaataa ggacagataa atttatggag
     6601 ccatttccat gactgtaaaa gtacttgctg aattcaattg tgtcgatgga aacgcggatg
     6661 aattcattga aatttgtcaa aatatttttc cagtgacgag ggagtttgat ggttgtagcc
     6721 atatcgatat atcagctgat caggaagatc caaatcgtct tataatgact gaaaattggg
     6781 catcaaaaga acatcatctg aaatatttgg aatataggac agatgacggg aatttggaga
     6841 agctcgtagc tcttttggaa tctcctcgga gatcacctac ttcgatttat cggatgcatg
     6901 gcctcatcag atagagtaca ccggattttt ctttaggaga tgtaccccat gatacattcg
     6961 tttgaaccag ataaatatta tctttcgatt ggctcccatc ctccagcttt gaaaataaat
     7021 agtggggata cagtgaatac aaacactgtt gatgccggtg gatttgataa aaagggaata
     7081 cttgtgtcag gaagaggtaa ccctcaaact gggcccatct atgtggaggg agcttgccct
     7141 ggggatgttt tagccgttag gttaaacaat atttggccta atcggggtca tgggttttgc
     7201 agacccaaaa tagcatccaa tgtgctggaa ccgggtcatg atagtagggt gacagattta
     7261 gacacattcg atttcctttt agattttgat tctaatacag cgaggattga aaatccttct
     7321 gaaggtctta cggatctatc agttccaatt cgtccgatga taggctgttt tggcgtggct
     7381 cctgatcgtg gtcaagccat ttcaactgct acttctggac cccatggtgg caatatggac
     7441 tatagaggtt ttgaaagtgg agtcactgcc tactttccta tttttgttga tggggcgctt
     7501 ttccatattg gagatggcca tgcctggcaa tctgatgggg aaatattggg aaccggaatt
     7561 gagatttcaa tggatgtgag ttttaccctt accgtaattc cgaagataga gatattttgg
     7621 cccagggggc aaaatgaaga ttttattttt actgccggga acgtgagacc attagacgag
     7681 gctctacaac atagtacttc agaaatgttg aggtggcttg ttaatgacta tgcgttgtct
     7741 tggccagacg ctaatatcat gttgggcacg tatgttcagt acgatgtagg gaatgttttt
     7801 gatccggcat acacaatgat ttgtaaaatt cctaaatcag ttctgccaag tgccagcccc
     7861 ggctccctga tatcacaatc tgcatagtta acctgcttaa gagcctctga accatttgtg
     7921 aaaactgtct aatttggctt tatcgcccgt aacagaaatc acgttactgt tgtaagcctc
     7981 gtgtatattt cttctggccg taatcagcaa ggcgaaatcc tggtatgtgg tttcaactgt
     8041 cgtgatagtt tcagtagctt tttcgatagg tcgatcaaat atgtgaagcg agccccgcca
     8101 ggtttgaatc cttaatgctt cactgtcatc aaaacaaaaa tcgattgtgg tagggtcgat
     8161 ttgttcaagg tctggagtgt acagccaatt agatatatta ggctctttcc atacctcaca
     8221 tagtaatttg gcctctgaat tttgcatttt gtacgaagaa tttagagatg aaaatatgtc
     8281 ccaagaatgt ataacaagtt ccagtagttt cattttgagc aataaatatg gggaaaattt
     8341 acctgcaggg tggtaagctg gaatatgcca gttttctgaa gcaatgtttt gaaaagcatt
     8401 tattagatcc cattccatat tggataatgt atcgagcaat tgttctaatt taggaaaagc
     8461 tgtttccgat atctgtatag cccgtgacgc aatcccatct gccatgcttc gagcattacc
     8521 agttcccggg tttggcatgc cttcagttgg atttccgtca ccctctatgg ctctagataa
     8581 agaattcagc aaaaacccat caatagccaa agtatgggaa gagacatctt ttacggccca
     8641 ctggtggcat ttactaggta tttcccattc ggttggtttt aagtctgcta gtccggttat
     8701 aaatttattt gacgattcgg caacgatcgc aagaaccgta ttggggtctt taagggttaa
     8761 catttttctg gtggcttcac tcttttcccc agatcaccag gtaccgcttt ttccattaca
     8821 ctgaacattt cctcagtgaa aaatggaagc cccgtagagg cgttaacacc accgtcaaca
     8881 gaaatgactt ctccatttat aaaactggat gcgtttgagg caagtaataa taaagctccg
     8941 gttagctctt caggtttgcc aattcttccc ataggggaat ttgcgctaat gtgatcgaga
     9001 aacatacctt tcttcaccca tgcaccagtc atctcactag ggaaccatcc aggtgcgatg
     9061 gcatttactc gtacccctcg gtctgcccag ttggctgcta aactttttgt tagtgtggtg
     9121 actgctcctt tagtcgcctg gtaggcaact ggcattcccc gcatgccatt aagaccggca
     9181 acagaagata gattgattat tgaacctcct cgtccatcac taagcattcg ccttcctccc
     9241 tccttacagc aataccacaa cccgattagg ttcactttga cagtttgttc aaacatataa
     9301 tcggtaacgt tttctggaac tgatccagcc tcggctatct gtccggcatt atttaccagt
     9361 atgtctactc ttccgaattt ttcttcagcc tttgtgaaca tattcgccac ttgggttgat
     9421 tctgttacat cgcattgggc caccattgaa gaaaaatttt cagagtcgaa tgattcctta
     9481 agggtgttta agttttcgat tcttcttgca gaaagcacta cattggcacc tctctcagcc
     9541 agtatctggg caaaggtgac tcccaagcca gaagaagcac ctgttattac ggcggtttta
     9601 tccttaacac tgaatatcgg atcatgataa agcatattta ttcccaataa ttcaattaat
     9661 agttatatat tcaataatca cacaatagat tttatcagta gggttaatac ttagaattgg
     9721 aattacctta aacaaagtaa taggcatacc aattcacctt ttgtgatctt gaattaaaga
     9781 tgtatttaac tttgtggtaa tcttccggaa tatcttgtta gctggcttca gatagttgtt
     9841 agtgcactaa tgtatgacac cttagtaggg acatttatag gatttctcag cggttcgata
     9901 ccgtggtctt tgatttgtgt gcgaactctt tcgggtaggg atgttaggaa agtaggggac
     9961 ggaaatccag gggccaccaa cgcatggata tcatcagggt ggatgattgg atcaatatct
    10021 atgctgctgg atgtattaaa agcagcagta cccgtatggg tagtcaccaa ccaatttaca
    10081 gggacctcat attctgagga ttttttgtca gtaacagtgg ttgctttatc tccaataata
    10141 ggacacgcct attccccttt gctgcgcttt agaggcggga aagctctcgc tccaagctgg
    10201 ggatcatgga ttgcccttac ttctggtatg gcgtttcctg tggcagttct cattctgggg
    10261 gtaatgcatc tgatgcagag aaatcatgca ataaccgtga cgacttgcct gatcggattc
    10321 acagttattt tcagtataat tgacagtcag gctttaataa tttttgggat cttgaatctg
    10381 atgttggtga cgttcaagca cagatccgaa tatcaacagg gaatagtttt tagagggcgg
    10441 gaaaggacat aaggtaaatg gaacttagta ccctaattac attcacctcg ggtttttttg
    10501 cctttacctc gtttttaatc acgatatgga attggcaata cctcgatagg ctcagactat
    10561 gccctagtaa tgagcttttt ggtgatactg atgtatcaat aacggtattg attccagcga
    10621 gaaatgaagg gaaaaatatc ggagaagtcc ttactgctct aagctgtcag actcacatta
    10681 attatgacgt tattgtttta aacgacggtt ctgaagacga tactgctgaa atagttagtc
    10741 aattccttgc atcggatctt gaaattcaat tgattgatgg agaaccaatc ccaaagggtt
    10801 ggttagggaa gaactgggct tgtcatcaat tgatgttgaa agccgacggc aactatattt
    10861 tgtttatcga tgcggatacc gttttgcacg aagacaccat caaagcagcc cttaatgaag
    10921 cgatacttga aaaatcggat ttattgacaa tgatccccgc cagatgtgca aacaatttta
    10981 ttgaaagaaa cctctacggg tttatagatt gggccattta tgcctggttg ccattacagt
    11041 tggcacactt gtcgaaaaac tcatatcttt cagctagcta cgggcagttt atgttattca
    11101 agcgagaatc ttatattttg agtggaggac ataggaaaat taaagacatc gccgttgatg
    11161 atttcgggct tgggcgttta attaaaagtt ctggtctgaa gtgggcattg aaagacggga
    11221 ccaatatggt cacttcgcta ccttacgatg acatattgga gactgtgaat ggtgtttctc
    11281 gaagcttggc tcctgcgctg aattatcgta tcagtttgat tttattgata tcaattttct
    11341 taatgggatt atttttcgca ccagttctta atctatattt ctacgttgcc aatggtggtc
    11401 agttcgaatt tttcgagctc ttgtctttgg taacaatatt tctgttactt ggatcatatc
    11461 tcttatcttg caagagattt tctcatagtc tttttattgt tgttttcatt catatcacag
    11521 tcatatttat gatacttatt gcatatcatt cattgttatc aaatttatta cgagttgcta
    11581 cttggaaaga tagaaatatg gtgattaaaa agttaaaaat ttaacaaaat attcacaaaa
    11641 tatgagctca ataacacata aatccccagg tatttatgta tagtggtacc tagtatgttt
    11701 aataatggaa aatatcacca cgcgatagat agatatcgaa cagtgactca agtttatttt
    11761 actgttaata actgttcttg tatgaagtaa caaggggtat ttgcctagtc gctttttggc
    11821 gaaatttaac ccccgccctg atccatagga cgggggtttt ttattgaatg caaaggagaa
    11881 ttggatatgg ctatcaatgg gctggctcat gccccagatg tgctcaagag agtgagaaaa
    11941 gaaaagggac ttacccaaaa agctgtggct gctcttgtgg gagttaatca aagcgtaata
    12001 tcaaggattg aggccggtga aataggcgca agcaatcgaa tagctgaagg gttagaagaa
    12061 gcatatggta tgcctattat agatgccctt ttatatagaa aaagctggac gggtgaacca
    12121 gaagacgtaa cagaaattaa acagcttatt gatcgatacc aaactgtctt aagtgattct
    12181 ggtataaaag ctataaaagc ttatgtggag catatgatag gtttgtctaa tggccctgat
    12241 tttttgccag gagggtcagc tataccaata aaaagtctgt ggggtggcga ctggacctgg
    12301 ggtccgcggg agttggaaag acatatcact gttgaatgtg cacctgtaat gacatggaac
    12361 agaatatcga ttcatcgttc ccaccctatt acag
//
LOCUS       DASCNS010000040         6904 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000359241, whole genome shotgun sequence.
ACCESSION   DASCNS010000040 DASCNS010000000
VERSION     DASCNS010000040.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000359241"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="QF838_01570"
     CDS             <1..712
                     /locus_tag="QF838_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJO59414.1"
                     /translation="MEVFQNSININTRGVLINSPNNPSGIVYDDKTLSEMCQIIKEKE
                     VEFGTEIYLVSDEPYRKIIFDDLEYNHIFNYHDRSIVATSHSKDLALPGERIGYIAVN
                     PSCPDLEELVDGLVFCNRTLGFVNAPALIQHLITHLQDTTIDVKVYEKKRDYLYEELT
                     DMGYSLIKPQGAFYMFPKSPIEDDVKFSEELKENRVLVVPGRGFGLPGYFRISYCMED
                     ATIRGSLEGLEKAISKYR"
     gene            complement(721..1836)
                     /locus_tag="QF838_01575"
     CDS             complement(721..1836)
                     /locus_tag="QF838_01575"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HJO59415.1"
                     /translation="MSHYEFECTECFRSHKADPQTLRCETCFGTLSVKYVNSKKQVNT
                     LVGGGTLEMPIPLCSLSEYLSLGEGNTPVVSMPNISSMFEVEAHAKLEFMNPTGSFKD
                     RGTAMMISALKSKGVHEIVEDSSGNAGASVSAYSTRAGMKAHIFAPLSAPRTKLNQIK
                     IYGSVLHEVDGSRDETTEAAEKFVKNKSIVYASHNLSPFFIEGTKTFGYEIFNDFDRE
                     MPENIVVPVGNGSLYLGIWKALEELKEKRLIKTLPKLHCIQAENVNPIASNNTWNKSE
                     MKSTVAGGIAVGSPPRKLEVQSVLRQSGGTANSVKEASIVEWQIKLAKYEGLFCEPTS
                     AAAFAGLENLINGKQIDKNESVLIPITGSGLKDVFPE"
     gene            complement(1843..3030)
                     /locus_tag="QF838_01580"
     CDS             complement(1843..3030)
                     /locus_tag="QF838_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO59416.1"
                     /translation="MKITDLKVWVTVPEKSSGDGSPSGRSYVFLRIDTNEGISGFGEA
                     TSSGGGGSIVVGNMIEFLRNSTAERDFRETIIGENPEHVELIWHKLFRRFTGGGGFGG
                     FVTTMLSGIDIALWDIKGKAAGKPVYEILGGGPVRKEIDLYTHVAPGDPVKAANQAKM
                     LVKQGFKTLKTDPFMPEMRQHHRKYMQGSISSEGAALATETIAAIREGVGESVDILID
                     CHGNFDVPTAIAMAKKLEPFNIGWYEEPVQPNSNQALKHVKESVSVPICVGERLYTRW
                     DFIPVLKDRLAEFLMPDILWTGGISEFHKIANLAESFYVPVSPHDASGPINIMAGAHT
                     MMTVPNFYKLEFNHAQLDSHNSLIDSPLNIVDGKLKMSEKPGLGIELDVEFIEAHLDE
                     DWK"
     gene            3121..4437
                     /locus_tag="QF838_01585"
     CDS             3121..4437
                     /locus_tag="QF838_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59417.1"
                     /translation="MQLPRHNDTESLEQGSQSKNVFYGWYLVVVGVFLLSISSLGVFR
                     GMSPMMPVLQNTFGWSRTQISLSSLLTRVEGAALGPVEGFLIDRIGARKMVLIGFSIM
                     AVGFVLFSSIQSIWQFYAVFILINLGNGIGGWLAVVTILNSWFKKKRTIAMAGAMSGV
                     LIAGIFVPPYTIALNHSFRLTVFFFGLIIIAIALPCVKIIKNHPEELGLEPDGDTPIS
                     KQLEPSSEVLLTETREDNEVEFTVGQALRTQVFWILTAAHVSSTISLATLSIHLMARL
                     QDIGLSSTTASYIELTSSVVALPSLFAAGWLGDKLSKKYLVAIFLFLQGISTIVLTVA
                     NGLPMALLFAILYGTAFGGRIPLMTAIRGDYFGRKAFASIMGWSMLPNGVLMAIAPVW
                     AAWMFDNYGSYTVPFISYAVINLAGAFVMLLVKKPKLSLSTSSLNS"
     gene            4447..5832
                     /locus_tag="QF838_01590"
     CDS             4447..5832
                     /locus_tag="QF838_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HJO59418.1"
                     /translation="MQQANSRGKGSDASRRDLTRGSVPRNLWNMAWPQMADSALTVVD
                     QIADLFWAGRMGYKTIAGLGVAQTYILIYSTARIGLDAGMRAMISRAIGANDREYANH
                     VLLQALTMTFIWTLVVGGLGIIFTDKLLSMVGVSGAVVESATMYMQLQFVAYSMQNFI
                     RLTSGALNAAGDSINPLKAVTVSRGIHILSSPFFIFGWLWFPDMGIAGAAAANIVAQF
                     LGLVMNLIVLHKGNSGLKLSFKSYRVDVILIWRLFRIGLPAAMTSMQRSVSQLVLLVC
                     VSRFGDAPVAAFALSRRVENIVNNTSRGLGRASAAVAGQNLGAGYLNRAKNSLSWALV
                     YSAILSAIAMTLYLLFTEEIAAVFNGDPNFIGHMTTWLIILAIATFPMSSVQVLTHGI
                     SNTGATVAPMNITLTTMWLIEIPMAFGLAFLTPLDSGGVPWGIACGNVVRCLAFLWYF
                     YRGSWLKPGVI"
     gene            5906..6400
                     /gene="lspA"
                     /locus_tag="QF838_01595"
     CDS             5906..6400
                     /gene="lspA"
                     /locus_tag="QF838_01595"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HJO59419.1"
                     /translation="MVWYRDWVFYSIAIVVFILDQISKEVIRRHLPLYGSWPEDGFLR
                     IVHGLNTGSAFGLFSGFTNLLTIASIVGIGVVLYYFQKQEISVIWYRLSIGLIVGGAL
                     GNLVDRLKDGAVVDFISVGWWPAFNVADSSISVGMVLLIITLLFGERFGWIPTSNESR
                     PDDS"
     gene            6397..>6904
                     /locus_tag="QF838_01600"
     CDS             6397..>6904
                     /locus_tag="QF838_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013045.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HJO59420.1"
                     /translation="MNEIKFVAQRDDIRLDQIVSCVIEAYSRSAARELIKKGLVKVDG
                     KIGKPSTKVAIGQQITAIIPEYQKTSLQPQQIELSVIYEDESLIVIDKPAGLSVHPGP
                     GHPDGTLINAVMALCPDISGVGEENRPGIVHRLDMDTSGVIVVAKNDRSHRSLSDQFK
                     NRTVTKKYV"
BASE COUNT         1824 a         1359 c         1539 g         2182 t
ORIGIN      
        1 tatggaagtt tttcagaact ctattaacat taatacaaga ggtgtattga taaattctcc
       61 aaacaatcca tcagggatcg tttatgacga taaaacctta tccgaaatgt gccagattat
      121 taaagaaaag gaagttgaat tcggtactga aatatatctt gttagtgatg aaccgtacag
      181 aaaaattatt tttgacgatc tcgaatataa tcacatattt aattaccacg ataggagcat
      241 agtcgctact tctcattcca aagatcttgc attgccaggt gaaagaatcg gctatatcgc
      301 agtcaatcca agttgtccag atttagaaga attggtcgat gggttagtgt tctgtaatag
      361 aaccctgggc tttgtgaatg cccctgctct tattcaacat ctgataactc atctacaaga
      421 tactacgata gatgtgaaag tatacgagaa gaaaagagat tatctatatg aagaactaac
      481 ggatatggga tattcattaa tcaagcctca gggtgctttt tatatgtttc caaaatctcc
      541 tatagaggac gatgttaaat tttctgagga gttaaaggag aatagagttc tcgtagtacc
      601 aggtcgcggt tttgggttac ctgggtactt ccgaatatcc tactgcatgg aggacgctac
      661 tatacgggga tctttagagg gtctagaaaa agcaatatca aaatatcggt aattaggaac
      721 ctattcgggg aaaacatctt ttaatcctga accggtaatt gggatcaaga cgctttcatt
      781 tttgtcaatc tgttttccat tgatgagatt ttcaaggcca gcaaatgccg ctgcagaggt
      841 gggttcgcag aagaggccct catattttgc caatttgatc tgccattcca caattgaagc
      901 ctctttaacg gaattagcag ttccccctga ttgacgcaac acactttgaa cctctagctt
      961 tcttggagga gatccgactg ctattcctcc tgccacagtt gacttcattt cagacttatt
     1021 ccaagtgttg tttgacgcta tcggatttac attttcagct tgaatacaat gcagtttagg
     1081 tagagttttg attaagcgtt tttcttttaa ctcttcaagc gctttccaaa ttcctaaata
     1141 aagggaccca tttcctacag gtactacaat gttttccggc atttctctat cgaaatcatt
     1201 gaatatttcg tagccgaatg tttttgtacc ttctatgaaa aaaggactga gattatgcga
     1261 ggcataaaca attgatttgt tcttaacgaa tttttctgcc gcttccgtag tttcatcacg
     1321 actgccgtct acttcgtgta aaactgaacc gtaaattttg atttgattta gctttgtgcg
     1381 tggggctgac aaaggtgcaa atatatgagc tttcatacct gctctggttg aatatgctga
     1441 tacggaggca ccagcattcc cagaagagtc ttccacgatt tcatggacac ccttagattt
     1501 caaagcggat atcatcattg cagtgcctct atccttaaaa gaccccgtgg gattcataaa
     1561 ttcaagcttc gcgtgagctt caacttcgaa cattgaagaa atgttaggca ttgatacaac
     1621 aggggtgttt ccctcaccga gtgagaggta ttcggatagg gagcataaag gaatcggcat
     1681 ctctaaggtc ccaccaccaa ctagggtatt cacctgtttc ttggaattga catatttgac
     1741 tgacaatgtt ccaaaacaag tttcacatct gagtgtttgc ggatcagctt tatgacttct
     1801 aaaacattcg gtacattcaa attcataatg ggacatattt ggttacttcc agtcttcatc
     1861 aaggtgggct tcgatgaatt ctacgtctaa ctcaattcct aatcctggct tttcagacat
     1921 ttttaattta ccgtccacga tattgagggg actgtctatc aaggaattat gagaatcgag
     1981 ttgggcatgg ttgaattcta atttgtaaaa attgggcaca gtcatcattg tgtgagcacc
     2041 ggccataatg ttgatgggcc cactggcatc gtgggggctg acgggtacgt agaatgattc
     2101 agccaggttg gctattttat ggaattcgct aattcctcca gtccatagaa tatcaggcat
     2161 caaaaactct gcaagcctat cttttaggac agggataaaa tcccatctgg tatacaaccg
     2221 ttctcctaca caaataggta ccgatacgga ttccttgacg tgtttcaagg cttggttgct
     2281 attgggttgt acaggttctt cataccaacc tatgttaaat ggctctaatt ttttagccat
     2341 ggctattgct gtaggaacat caaaatttcc gtgacaatct atcaatatat ctacgctctc
     2401 tccaacgcct tctctgatag ctgctattgt ttctgtggcg agtgccgcac cttctgaaga
     2461 tatggaacct tgcatatatt tcctatggtg ttgtcgcatt tcaggcatga aggggtctgt
     2521 tttaagcgtt ttgaatcctt gcttcaccaa catttttgcc tgattcgctg ctttaactgg
     2581 atctcctgga gccacatgag tatagagatc tatttctttt cggactgggc ccccacctaa
     2641 tatttcatat actggtttgc cggcagcttt accttttatg tcccagagcg caatatctat
     2701 tccgctcaac attgtggtaa caaaaccacc gaaccccccc ccacctgtga atcgcctaaa
     2761 taatttatgc caaatcagtt ctacatgttc agggttttca cctatgatcg tttctctgaa
     2821 atccctttca gcagtggaat ttctcagaaa ttcgatcatg ttaccaacta cgatgcttcc
     2881 gccgcctcct gagctggtcg cttccccgaa accagaaata ccttcgttag tgtctattct
     2941 caagaacaca taacttcgcc cactcgggga cccatcaccg gaggattttt ccggaaccgt
     3001 tacccacact tttaaatctg ttattttcat gaataaaagc cttccataaa ccacttaatg
     3061 gtatccaata aaaaggatcc aaatctgtaa cttcaatacc tttctgttac catcttcagg
     3121 atgcaacttc cgagacataa cgacaccgaa tctttagaac agggttctca atcaaaaaat
     3181 gttttctatg gttggtatct tgtcgttgtt ggtgtttttt tactgtccat tagctcactt
     3241 ggtgttttta ggggcatgag tcctatgatg cccgtgcttc agaatacatt tggctggtct
     3301 agaacacaaa tttccttaag ttctcttttg acccgagtag aaggggccgc cctaggccct
     3361 gttgaaggtt ttttaattga tcggattggt gcccgaaaaa tggtactgat tggattttcc
     3421 attatggctg ttggatttgt acttttttca tctatccaat caatatggca attttatgct
     3481 gttttcattt taataaatct tggcaacggt ataggggggt ggttggcggt agttactatt
     3541 ttaaatagtt ggtttaaaaa gaaacgaaca atagcgatgg ctggtgcaat gtcaggggtt
     3601 cttatcgccg gcattttcgt tcctccctac acaatcgcac tgaaccatag ttttcgacta
     3661 accgtgtttt tcttcgggct cattattatt gcgattgcgc taccttgcgt gaaaatcatt
     3721 aagaatcatc cagaggagtt gggtctcgag ccagatggag atactccaat ctcaaagcaa
     3781 ctggaaccct cttcagaggt tttgctaact gaaaccaggg aagacaacga agtggaattt
     3841 acagtagggc aggctctgag gacccaagtt ttttggattc tcacggctgc acatgtatct
     3901 tctactattt ctctggctac tctgtcgatt catttgatgg cccgattgca ggatattggc
     3961 ctaagttcaa ctaccgcgag ttacatagaa ctgacatctt ctgtagttgc cttacctagc
     4021 ctttttgcag ccggatggct gggagacaaa ttatcgaaga aatacttagt ggcgattttt
     4081 ttgtttttgc aagggatatc caccatagta ttgaccgtag cgaacggttt gccgatggcc
     4141 ttattgtttg cgatattgta tggaacagcc tttggaggac gaatacctct catgactgca
     4201 ataaggggtg attattttgg tagaaaagct ttcgcttcca taatgggttg gtctatgcta
     4261 ccgaacggcg ttttaatggc catcgctcca gtatgggccg cttggatgtt tgataattat
     4321 ggcagttata ctgtcccatt tatttcctat gcggtaataa acttagccgg cgcttttgtc
     4381 atgttactag ttaaaaaacc caaattgtca ctctcaacta gttctttgaa ttcctaaagt
     4441 gtcttaatgc agcaagccaa ttcgagaggg aagggcagtg atgcctctag gcgagacctg
     4501 acacgcggta gtgttcccag aaacttgtgg aacatggctt ggcctcaaat ggccgacagt
     4561 gcccttactg ttgtggacca aattgcagat cttttttggg ctggcagaat ggggtacaag
     4621 acaatagcag gtcttggggt agctcaaact tatatattga tttactccac agctaggata
     4681 ggcttggatg ctggcatgag ggctatgata tctcgggcta ttggggcaaa tgatagagag
     4741 tacgcaaacc atgtgttact acaagctttg acaatgactt tcatttggac tttagtggtt
     4801 ggtggattag ggatcatttt cacagataaa ttattgtcga tggttggagt aagcggggct
     4861 gtggttgaat cagcaaccat gtatatgcaa cttcaatttg tggcctattc gatgcaaaat
     4921 tttatacgcc tcaccagtgg ggccttgaac gcagcgggag acagtataaa tccactgaag
     4981 gctgtaactg taagccgtgg aattcacata ttgtcgagcc ctttctttat ttttgggtgg
     5041 ttatggtttc ctgacatggg tatagctggc gctgctgcag caaacatagt ggctcagttc
     5101 ctgggtctgg ttatgaattt aatagtgtta cacaaaggaa attcaggttt aaaactgtcc
     5161 tttaaatcct atcgagttga tgtcattttg atatggagat tgtttcgaat cgggctgcca
     5221 gctgccatga cttctatgca aagatctgtt tctcaattgg tccttttggt ttgcgtctca
     5281 cgctttggtg atgccccagt tgcggccttt gctctgtcaa gaagagttga aaatatagta
     5341 aataatacta gcaggggttt gggtcgagct tcggctgcag tagctgggca gaatttgggg
     5401 gctgggtacc tcaatcgggc taagaactct ttgagctggg cattagtcta ttctgccatt
     5461 ttaagcgcta tagcgatgac tttgtattta ttgtttacag aagaaattgc tgctgtattt
     5521 aatggggacc ccaattttat agggcatatg actacctggt taatcatact tgccatagcc
     5581 acttttccga tgagctcagt ccaagtttta acacacggta taagtaacac tggtgcaact
     5641 gtggctccaa tgaatataac ccttacaact atgtggttaa ttgaaatacc tatggctttt
     5701 gggttggctt ttttaacacc cttggattct ggaggagttc catggggcat cgcttgtggg
     5761 aatgtggtga gatgtttagc gttcctttgg tatttttata gagggagttg gcttaaaccg
     5821 ggtgtaattt aaacccgata caatatgatg taatagcatc agttactact taaggtgctc
     5881 ataagttcat caaagtttaa agaatatggt ttggtataga gactgggttt tctacagcat
     5941 agctatcgta gtgtttattt tggatcaaat ttccaaggag gtgattcgcc gccaccttcc
     6001 cttatatggt tcttggccag aagatgggtt cttaaggatt gttcacggat tgaacacagg
     6061 gtctgcattc gggcttttct ctgggttcac taacctcctg accatagcat ctatagttgg
     6121 gatcggggtg gttttgtatt attttcagaa acaagagatt tcggttatct ggtatcggct
     6181 gagtatagga ctgatagttg gaggggcttt aggaaatttg gtggatcgtt tgaaagatgg
     6241 ggcagttgtg gatttcataa gtgtcggatg gtggcctgct tttaatgtgg cggattcttc
     6301 gataagcgtc ggaatggttt tattgattat taccttgttg tttggagaaa gatttggttg
     6361 gatccccact tcaaatgaaa gtcgacctga tgattcatga atgaaataaa atttgtggcc
     6421 caacgagacg atattagatt ggaccaaatt gtttcgtgtg ttattgaggc ttattctcgt
     6481 tctgcggcaa gggaactgat aaaaaaaggt ttagtgaaag tggatgggaa gataggaaaa
     6541 ccttcgacaa aggtagctat cggacaacaa atcacggcta ttattccgga gtaccaaaag
     6601 acttctctgc agccacagca aatagagtta tcagtcatat atgaggacga atcactcatt
     6661 gttattgata aacctgctgg gctttctgtt cacccaggac ctggccaccc tgatggtacc
     6721 ttgattaatg ctgtaatggc tttatgtcct gacataagcg gagtgggtga agaaaatagg
     6781 ccaggcattg tacatcgact agatatggat acttctgggg taatcgttgt tgccaaaaac
     6841 gaccgaagcc atagatcttt atcagatcag tttaagaatc gcacggttac taaaaagtat
     6901 gtgg
//
LOCUS       DASCNS010000041        11319 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000365380, whole genome shotgun sequence.
ACCESSION   DASCNS010000041 DASCNS010000000
VERSION     DASCNS010000041.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11319)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000365380"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            95..469
                     /gene="folB"
                     /locus_tag="QF838_01605"
     CDS             95..469
                     /gene="folB"
                     /locus_tag="QF838_01605"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HJO59421.1"
                     /translation="MKNQDQIILKGMIFYGYHGVNPEERLIGQKFVVDVTVECSLLQP
                     SLSDMVCDTVSYSDLFKTVKSIVEGPPHNLLESVAKNISDSIILGYDIESISVTIKKP
                     DVPINGANLDYAGVTLTRTKGK"
     gene            493..1893
                     /locus_tag="QF838_01610"
     CDS             493..1893
                     /locus_tag="QF838_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HJO59422.1"
                     /translation="MPANLPETLGALVESGYKSRSVKEEIRTNLVQRISGGLEVFPGI
                     VGFDQSVIPQIQNAILAGQDIILLGERGQAKSRIIRLMVDLLDEHIPFIKGSEINDDP
                     LHPISQYGIEILAEDKNDTKLGWLSRDDRYSEKLATPDVSVADLIGEIDPIKVAEGRY
                     LSDALAIHYGMIPRTNRGLFCINELPDLTERIQVSLFNLMQERDIQIKGHQVRLPLDM
                     IIVATANPEDYTNRGRIITPLKDRYGAQIRTHYPAEVSQEMAIVKQEYKKFSDSDQLV
                     KTPEFMKDIIGEITHLARRSPEINQRSGVSLRVSISNFETMIGQAFRRSLATHSVSSP
                     RISDLSYLIASTIGKVELETVEEGLETKIIGDIVDRAISNVFAKYTDPDEFDFLLAKF
                     EEGLTVLSGSSISDDEYLETIKDCGILEDKLISLCNPMADSSAIISALEFILEGLYLG
                     SKLSKDSHNSTVKYSI"
     gene            1961..4633
                     /locus_tag="QF838_01615"
     CDS             1961..4633
                     /locus_tag="QF838_01615"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HJO59423.1"
                     /translation="MAQNNDSLNMSRAYSPADTESRIYKFWEDSGYFKPDTSSNKPPF
                     VMIMPPPNVTGELHMGHALTVAIEDMIVRWHRMKGEPTLYLPGTDHAGIATQVVVERL
                     LATEGLNRYQLGREKFEARVWDWVETYGTRIYEQLRRLGTSCDWSRSKFTLDEIPRAA
                     VRKTFVDLYEKGLIYRGERIANWCPRCSTALSDLEVKYKEVNGNLYQLRYPFSDGSGF
                     LVVATTRPETMLGDTAVAVNPEDERFKRYIGKEIKLPVADRTIPIVADEAVEMEFGTG
                     ALKVTPAHDPVDFEIGLRNDLPVITVIGNDGKMNSAAGEFQNLDRFDCRKLIVDQLTA
                     QGLLIETESIQHSIGHCDRCDEIIEPLISKQWYISMESLAAPAIKAVKDNQIRIIPER
                     FTNVYFNWMENIRDWPVSRQLWWGHQIPVWYCEDCLSETVTQKDPQTCKSCGSSKLQQ
                     DQDVLDTWFSSGLWTHSTLGWPDDTEDLKSFYPGSVMETGYDILFFWVARMIMLGISN
                     MGQIPFHTVYLHGLVLDTEGVKMSKTKGNVLNPLDLIDEYGADAVRFALTTGTSAGNN
                     LRMNEQKLESSRNFANKLWNASRFVISNINKREDSIKIIKPENISNRHDKWILSRLDQ
                     LTTNVNRLMGDFQFGEAQTHIHDFLWNEYCDWYIELTKDRLRTQNVDSPLSILAYVLE
                     QTLRLLHPFLPFVTEEIWANLCKSLDTPKDWPDSLITANYPVNPETTIDESTIMEMGK
                     LIDFIRAIRNMKSELNIQQRQTIECRIASEVESSLVESEKATLENFAGLAISEISNST
                     LLQMKNGEISIVENLGTAYITLGESVDLSHELERLRTERDELSKYISSLNARLSNQSF
                     VDKAPEEVVEKEKERLKRSNDRETRINEILQTLN"
     gene            complement(4657..7296)
                     /gene="secA"
                     /locus_tag="QF838_01620"
     CDS             complement(4657..7296)
                     /gene="secA"
                     /locus_tag="QF838_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HJO59424.1"
                     /translation="MAFLKAITNILGSSDEKALDKLRPLVEQINALEALYEAKTPEEL
                     KDVANTLRGAFGEGKSLDDLLPDAFAAVREAAKRALSQRHFDVQLLGGIVLHQGKIAE
                     MRTGEGKTLVATLPAFLNSLSGQGVHVVTVNDYLAKRDAEWMGAIYNSLGVSVAALQH
                     DYASLFHPDNFDGTTVKEKGITRKEAYGADITYGTNNDFGFDYLRDNMVDIAERRVQR
                     GRAFAIVDEVDNILVDEARTPLIISGPSQQNPSEYSKYAKIVPSLREEEDYSVEEKHR
                     SVSLSQNGIAKIEKFLKVDNLYDTENFGQAHFVENAVRAEVIYKKDREYVVKDGKVVI
                     VDEFTGRLMEGRRYSDGLHQAIEAKENIVVQRESVTYATITLQNYFRLYSKLSGMTGT
                     AATEAEEFWKIYKLEVLSVPTNNPVMRTDHGDLIYKDQTAKYSAVVKEIKSRSEKGQP
                     VLVGTTDIDRSEVLSSMLKKQGVKHEVLNAKQHDREATIVAQAGRPGAVTVATNMAGR
                     GTDIVLGGAMDKDSEDVDGWEKDHTHVLSVGGLYIIGTERHEARRIDNQLRGRSGRQG
                     DPGETRFFCALDDDLVRRFGGDRIQTIMDWAGMDDETPIENKMISRSIEGAQVKVESF
                     HFDMRKHLVEYDDVVNSHRDVIYEQRNAVLDGVELRPKIQRMIEEECEAIIADGMSSS
                     ATENWDIQGLTTDVQRIISLPGDLIDSEYVFNSGQRNYSDQVREQIEKTYNSFEAVLG
                     DDNARSIERYIMLRTIDANWVQHLTAMENLRQGISLQAVGQRDPLVMYKKEGHELFQN
                     LQSKIIDDVVHTIFKTGTPIDAESGIQPRSNPKIVNQNKSVFKTNDSQKTPKRPVAAK
                     VGRNEQCPCGSGKKYKRCCGLAT"
     gene            complement(7388..8338)
                     /locus_tag="QF838_01625"
     CDS             complement(7388..8338)
                     /locus_tag="QF838_01625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HJO59425.1"
                     /translation="MPSFEDLRIFSGNSHRKLAADICEYLRIPLGECDVFKFSNDNTF
                     VSFKENIREKDVFLLQTFCRPVNDHIMELLVMIDAAKRASAGRITAVIPHYSYGRSDK
                     KDQPRVPITARLVANLLTVAGADRVLTVDLHAGQIQGFFNIPVDELSAVQMLAGYFKS
                     NDMRIEVASATDAGDVKRTKDTARFLNVGMSLVEKHRIGNEDRVEVVSLIGDVENKTA
                     LIVDDELGTGGTVIATAQALKKHGARDIYCAVTHPVLSGEASNLLENSDVIETVVTDT
                     LPVPDEKQGSKLTVLSIAPMLGEAIHRIHGGLSVGAMFES"
     gene            8533..9771
                     /gene="amrB"
                     /locus_tag="QF838_01630"
     CDS             8533..9771
                     /gene="amrB"
                     /locus_tag="QF838_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HJO59426.1"
                     /translation="MPRQLPLKPQLRDLSPQWIQRQDGVFLHLEDDLGLAKTAVQIPQ
                     NLTPILLLCDGTRTLSSINGGLLLQGISIGEERISNLIQQLDDALLLENGKFAGEKDK
                     AIHKYRNSKHRPMSFAGTIYPAITSDLDLFIEESKSKFGPSGQSNQYDGTLQGVLSPH
                     IDFARGYATYAKLWRESEQDLDGIEQVVILGTDHSGGLGSITPTMQNYQTPYSELETD
                     LEGVRLIEAAIVGKDPYNEELHHIKEHSIELALTWLHNSMGGKQFKLLPILCGSFQKF
                     IIDKQQPDSDETIKNIIDSLFEYRKRARTLVIAAGDLAHVGPEFGDATSMDELSLGDL
                     ASKDHISLANIEKGDPDSFFQASAEEGDVRKICGLTPIYLMGKVLSDCKGYSVGYDQC
                     PADQNRTSVVSIAGTLLFGH"
     gene            9784..10107
                     /locus_tag="QF838_01635"
     CDS             9784..10107
                     /locus_tag="QF838_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HJO59427.1"
                     /translation="MKRVGFLLKVREKYIEEYKTHHQNVWEEMKSALVRHGWNNYSLF
                     IRPDGLLFGYFEANESFSDSLTGMSKEQINTEWQELMSPYFEILEGSDPDQSIVELEE
                     VFHLD"
     gene            10124..10885
                     /locus_tag="QF838_01640"
     CDS             10124..10885
                     /locus_tag="QF838_01640"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO59428.1"
                     /translation="MRLKSKKVIVTGGASGIGRAICTVFAAEGASVVIADIDEPGSME
                     TLSLIQSAGGHAAAITTDVSSEESVAKMISASESFLGGIDILVNDAAAFVFGKLEDVS
                     RKDWEVVFGVNVIGASNTVKHSLPFLKKSASPNIVNIASVSGFIAQPAFVPYNASKGA
                     LLQLTKCMAMDLSEFKIRVNSVCPGAIYTPATERHIAFENADRDEFLKKAGEGSFLKR
                     VGRPEEVAYAALFLASDEASFITGTQLVVDGGATV"
     gene            complement(11126..>11319)
                     /locus_tag="QF838_01645"
     CDS             complement(11126..>11319)
                     /locus_tag="QF838_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59429.1"
                     /translation="DYYDGDDDDDDDDADDDDDDKGGDNDDADDDVLLHGLLHYLLGL
                     HVQLWRLGTLCFDKLLATI"
BASE COUNT         3430 a         2416 c         2305 g         3168 t
ORIGIN      
        1 cgacttcttt gaacggtcaa gtggtggacg tgcctgtggt taaaagggcg gaatctctac
       61 ttaacctagc taggcaaatg gatcttgttt cttgatgaaa aatcaagatc aaatcatact
      121 aaaaggtatg atattttacg gatatcatgg tgtaaatcct gaagaacgtt tgatagggca
      181 aaaatttgtt gttgatgtaa cagtcgaatg ttccttattg caaccgtctt tatcagacat
      241 ggtttgcgac acagtcagtt actctgattt attcaaaact gtaaaatcca ttgtggaagg
      301 accacctcac aatctcctag aaagtgttgc gaaaaacatt tcagactcaa taattctcgg
      361 ctatgatatt gaatcaatat ctgtgacaat taaaaaacca gacgtaccaa taaatggagc
      421 aaatctagat tacgcagggg ttaccctaac ccgtaccaaa ggtaaataaa attcatttaa
      481 gaaggtttaa tagtgccagc aaatctacca gaaacattag gtgcattagt agaatcgggt
      541 tacaaatccc gttcagtcaa agaagaaatt agaaccaatt tagttcaaag aattagcgga
      601 ggcttggaag tatttcctgg gatagttggt tttgaccaat ccgttatccc acagatacaa
      661 aacgccattc ttgctggtca ggatattatt ctgttaggtg aacgaggtca ggccaaatca
      721 agaataatcc gccttatggt ggatttgctg gatgagcaca tcccctttat caagggctct
      781 gaaataaatg acgatcctct ccatcctata agccaatacg gtattgaaat tcttgccgaa
      841 gacaaaaatg atacgaaatt aggttggctt tccagagatg accgctattc ggaaaagctt
      901 gccaccccgg atgtttcagt ggcagattta ataggggaga ttgatcctat taaagtagct
      961 gaagggcgat atttatcaga tgcattagca attcattatg gaatgatacc tcggacaaat
     1021 agaggcttat tctgtataaa tgaacttcca gatcttacgg aaaggataca ggttagctta
     1081 ttcaatttaa tgcaagagcg agatatacag atcaaaggtc accaggtgag attgccttta
     1141 gatatgataa tagtagccac tgccaacccc gaagattaca caaacagagg ccgcattatt
     1201 accccgctca aggacaggta cggagcccaa atccgaaccc actatccagc tgaagtttca
     1261 caggaaatgg caatagtaaa gcaagaatat aagaagttct ctgattccga ccaattagtt
     1321 aaaactcctg agttcatgaa ggacattatc ggtgaaataa cacatttagc ccgtaggagt
     1381 ccagaaataa accaaagatc tggggtaagc ctaagagttt cgatttctaa tttcgaaaca
     1441 atgatcggac aagcctttag aagaagtcta gctacccact cagtatcctc acctagaatt
     1501 agtgacctct catacttaat agcatcgact atcggcaaag tggaattaga gactgtggag
     1561 gagggtttgg aaacaaaaat tattggcgat attgtcgata gagcgatttc aaacgtcttc
     1621 gctaaataca cagacccaga tgagtttgac ttcttgttgg cgaaatttga ggaaggatta
     1681 acagtgctat ctggcagttc aatatcagac gatgaatatt tagaaacaat taaagactgt
     1741 ggaatattgg aagataaatt gatttcgctg tgtaatccga tggcagacag cagcgctata
     1801 atttctgcac ttgagttcat tttagaagga ctatatctcg gtagcaagtt aagcaaagac
     1861 tcacacaaca gcacagtgaa gtattcaata taagttatcc gacatatttt acaaaccaat
     1921 ttaaatttct tgatttataa tttgatggtc tggagtaata atggcccaga ataacgattc
     1981 attaaacatg tccagagcat atagccccgc ggatactgag agcaggatct acaaattctg
     2041 ggaggattca gggtacttca aaccagatac cagctccaat aagccacctt ttgtcatgat
     2101 aatgcctccc cctaacgtaa ctggtgaact tcatatgggt catgcactaa cagttgcaat
     2161 tgaagacatg atagtacgct ggcatcgaat gaagggagaa ccaactttat atttgccagg
     2221 tacagatcat gctggtatag ccactcaagt agttgttgaa cgattactag caacggaagg
     2281 acttaatcga taccaacttg gtagagaaaa atttgaggct cgggtatggg actgggttga
     2341 aacatatggg acccgaatct atgagcaact tcgtaggctc ggaacttcat gtgattggtc
     2401 tcggagcaaa tttacacttg atgaaatacc tagagccgca gttcggaaaa cattcgtgga
     2461 tctttatgag aagggattga tttatagggg agaacgaata gctaattggt gcccaaggtg
     2521 ctcaactgcc ttatctgact tagaagtcaa gtataaggaa gtcaacggaa atttatatca
     2581 gctacgatat cctttttcag acggatcagg atttctcgtg gtagctacca cgagacctga
     2641 gacaatgctg ggagacaccg ccgttgcagt caatccagaa gacgaaaggt tcaaaagata
     2701 tataggaaaa gaaataaaat tacccgtggc tgatcggacg atccccatag tagctgatga
     2761 agctgtagag atggagtttg gtacgggagc cttgaaagtc actccagcac acgatccggt
     2821 cgactttgag ataggtctca gaaatgacct tcccgtaata actgttatcg gaaatgatgg
     2881 gaaaatgaat tctgcagcag gggaatttca gaacctagat agatttgact gtagaaaact
     2941 gattgtagat cagttaacgg cccaagggtt attaattgaa acggagtcaa tccaacactc
     3001 tatcggccat tgcgataggt gtgatgaaat aatcgaaccc ttgatatcaa agcaatggta
     3061 tatatcaatg gaatctttgg cagcacctgc aatcaaagcg gtaaaagaca accaaatacg
     3121 gatcattccg gaaaggttca caaacgtata ttttaactgg atggaaaata ttcgcgattg
     3181 gccggtaagt aggcagttat ggtggggcca ccaaatacct gtgtggtatt gcgaagattg
     3241 tttgtccgaa acagtcaccc agaaggatcc acaaacctgt aaatcctgcg gatcatcaaa
     3301 gttacaacaa gaccaagacg tactcgatac ctggtttagt tctggattat ggacacattc
     3361 gacactcgga tggcctgatg acaccgaaga tttaaaatct ttctatcccg gttcggtgat
     3421 ggagacagga tatgatattt tgtttttctg ggtggcaagg atgattatgt taggaatatc
     3481 caatatgggc caaattccat tccacacggt ttaccttcac ggtcttgttc ttgacaccga
     3541 aggcgtgaaa atgagtaaaa ccaaaggtaa tgtgctcaac ccccttgatc ttatcgacga
     3601 atatggagcg gacgcggtga ggtttgcatt gacgacaggg acatctgccg gaaataacct
     3661 aagaatgaat gagcagaaac ttgaatcaag ccgtaatttc gccaataaac tatggaacgc
     3721 ctccagattt gttatttcaa atatcaacaa aagagaagac tctatcaaaa ttatcaaacc
     3781 cgaaaacatc tcaaatcgtc acgataaatg gatactctca cgacttgacc agctaactac
     3841 caatgtcaac aggctgatgg gggattttca atttggtgaa gctcagacac acattcacga
     3901 ctttctatgg aatgaatatt gtgattggta cattgaatta acaaaggaca ggttgagaac
     3961 ccaaaatgtt gattccccat tatctatttt ggcatacgta ttggagcaaa cccttagatt
     4021 attacaccca ttcttgccat ttgttacaga agaaatttgg gctaatttat gtaaatcact
     4081 ggatacacca aaagattggc ccgactcttt aatcacagca aattatccag ttaatcctga
     4141 aacaacaata gatgaatcta ccatcatgga aatgggtaaa ttgatcgatt ttatccgtgc
     4201 gataagaaat atgaaatcag aattgaacat tcaacagaga caaacaatag aatgcagaat
     4261 cgcctctgag gtggaatcaa gcttggtgga atctgaaaaa gccaccctgg aaaacttcgc
     4321 tggccttgct atctcagaaa ttagcaattc aaccttactt cagatgaaaa acggagaaat
     4381 ttccattgtt gagaatctgg gcaccgctta tatcacgtta ggagaaagtg tggatttaag
     4441 ccacgaattg gaacgcttac ggacggaacg ggatgaacta tctaaataca tctcctcttt
     4501 gaatgcacgt ttgtccaatc aatctttcgt cgataaagca ccagaagaag ttgttgaaaa
     4561 agaaaaggaa aggctaaaaa ggtctaatga tcgagaaaca cgaataaatg aaattcttca
     4621 gactctaaat taggtaattg aaaacttgat ttgggcttaa gtcgccaacc cgcagcatcg
     4681 tttgtatttt ttaccacttc cacatggaca ttgttcattt ctccctactt ttgccgccac
     4741 aggacgtttg ggagtttttt gcgaatcatt agttttgaaa actgacttat tctgattgac
     4801 tattttggga ttcgaacggg gttgaatacc tgattcagcg tctattggag ttcctgtttt
     4861 gaatatggtg tgaacaacat catctattat tttggactgt aaattttgga acagctcatg
     4921 accttctttc ttatacataa ctaacgggtc tcgttgcccg acagcctgca ggctaatacc
     4981 ttgacgaagg ttctccatgg cggtgaggtg ttgtacccag tttgcatcga tcgtcctaag
     5041 cattatgtat ctttcaatgg atcgagcgtt atcatctcca agaaccgctt caaaactatt
     5101 gtatgttttt tctatctgtt ctcttacctg atcagaataa tttctttgtc ccgaattgaa
     5161 aacatattcc gaatcgatca aatctccagg aagagaaatt atcctttgaa catccgttgt
     5221 caaaccttga atatcccagt tttctgtagc agaactcgac ataccgtcgg cgattatagc
     5281 ctcacattct tcttcaatca ttcgttgtat cttaggtcgt agttctacac cgtccaaaac
     5341 agcatttctt tgttcataaa tgacatctct atgggagtta actacatcgt catattccac
     5401 taaatgcttc cgcatatcaa aatgaaacga ttcaacttta acttgagcac cttcgatgga
     5461 tctggaaatc atcttatttt caattggggt ttcatcatcc atccctgccc aatccataat
     5521 cgtctgaatc ctatctccac caaatcttct gactaagtca tcatccaagg cacaaaaaaa
     5581 tctggtctct cccggatcac cttgtcgccc agatctaccc ctcaactgat tatctattct
     5641 tcgagcttcg tgcctttctg taccgattat ataaagccca ccgacactca agacgtgggt
     5701 gtgatccttc tcccatccgt ccacatcctc tgaatcctta tccattgcac caccaagaac
     5761 tatatcggtc ccacggccag ccatatttgt tgcaactgtt acagccccag gacggccagc
     5821 ctgggcaaca atcgttgcct ctctatcatg ttgtttcgca ttcaacacct cgtgcttaac
     5881 gccttgtttt ttcagcatag aacttagtac ttccgaccta tctatgtcag tcgtgccaac
     5941 caatacaggt tggccttttt cggatctaga tttgatttct tttactacag cgctatattt
     6001 ggccgtttga tctttgtata tcaaatcccc gtggtcagtt ctcatgaccg gattgttagt
     6061 agggacacta agaacctcca acttataaat cttccaaaat tcctccgcct cagtcgccgc
     6121 agtaccggtc attccggata gtttggaata caatctaaag tagttttgaa gggtaatcgt
     6181 ggcataagtc acagattccc tttgaacaac aatattttcc ttcgcttcga tggcctgatg
     6241 aagtccatcg gaataccttc taccttccat cagacgccct gtaaattcat ccacaatcac
     6301 cactttccca tctttcacca cgtattctct atcttttttg taaatgactt cggcccgcac
     6361 agcattttct acaaaatgag cctgcccgaa attttcagtg tcgtacagat tgtctacttt
     6421 tagaaatttt tcgatctttg ctatcccgtt ttggcttaaa gagaccgatc ggtgcttttc
     6481 ttctacagaa taatcttcct cttctctcag actgggaacg atcttggcat atttggaata
     6541 ttcactagga ttttgttgag atgggccact tattatcaag ggtgtcctag cttcatcaac
     6601 tagaatatta tcaacctcat ctactatcgc gaaggctcgt cctctttgaa ctcttctttc
     6661 ggcaatatcc accatattgt cccgtaggta atcaaatcca aaatcgttat ttgtaccgta
     6721 agttatatct gccccataag cctcttttcg ggtaatccct ttttctttaa cagtagttcc
     6781 atcaaaatta tcaggatgaa ataaggaggc ataatcatgc tgtaaagcag cgacggaaac
     6841 tcccaatgag ttgtagattg ctcccatcca ctctgcatct cttttagcca agtaatcatt
     6901 cactgtgaca acatgaacac cttgcccaga tagtgaattg agaaaagccg gcaaggttgc
     6961 caccaaagtt tttccttcac cagtcctcat ttcggcaatt ttaccttggt gcaaaactat
     7021 cccgcccagt agttgcacgt cgaaatgtct ttgactcaaa gcccttttgg cagcctctct
     7081 taccgcagcg aatgcatcag gtagaaggtc atcaagcgac ttcccttcgc caaaggcacc
     7141 ccttaaggta tttgctacgt ctttcaattc ctcaggcgtc ttcgcctcat acagcgcttc
     7201 taaagcgttt atttgctcca ccaaaggcct aagtttatcc agggcttttt cgtcagagct
     7261 acccagtatg tttgttatcg cttttaaaaa ggccattgtg tgttgttacc agtcccgtat
     7321 ttcaagaaga attatacgac tagcacattt atcagtacca gcggttatga tccaaggtta
     7381 atcaagtcta agactcaaac attgccccaa ctgagaggcc accatggatc ctgtgaatcg
     7441 cctctcccag cataggagct atcgaaagaa ctgttaattt cgagccctgt ttttcatctg
     7501 gtaccggtaa tgtatcagtc accacagttt caatgacgtc gctgttctcc aataaattac
     7561 tggcctcacc tgatagtact ggatgagtta cggcacaata aatgtcccgt gccccgtgtt
     7621 tttttaaagc ctgtgctgtt gctataacag ttccgcctgt acctaactcg tcatcaacga
     7681 tcagggcagt tttattttca acatctccaa tcaaactaac gacttcaact cgatcttcat
     7741 tgcctatcct atgcttctcc accaatgaca taccaacatt caaaaatctt gcagtgtctt
     7801 tagttctttt tacatccccc gcgtcagtag cagaagccac ctcgatcctc atgtcgttgc
     7861 tcttaaagta cccagctagc atttgtacag cggataactc gtcaactggt atattgaaga
     7921 aaccctgtat ctgaccggcg tgaaggtcga ccgtcaaaac cctatccgct ccagcaacgg
     7981 taagtaagtt tgcaaccaga cgagccgtta ttggtaccct aggttgatct ttcttatcgc
     8041 tacgtccata ggaatagtgt ggtataacag ccgttattcg accagcagac gctcttttcg
     8101 cagcatcaat cataaccaaa agttccatta tatggtcatt aactggtcta caaaaagtct
     8161 gcaacaagaa gacatccttt tctctgatat tttctttaaa actcacgaat gtgttgtcat
     8221 tactgaattt aaaaacgtca cattcaccta aaggaatacg taaatattca catatatcag
     8281 ctgcaagttt ccgatgggaa ttaccagaaa aaattcttaa atcttcaaaa gagggcatat
     8341 tacaatcatc tacatagtat tttggtatgg ataacaacct attttggttg tgttcaaccg
     8401 gtcaagcgag gcgaattata gcacagtaaa atacacccaa tcgcacatgt tcactaacta
     8461 gtattgctta ttatgccaag gtaactgata cttttctttc ctattgttag gacgttaatc
     8521 aggacaaaaa gaatgccaag acaattacca ttaaaacctc agttgagaga tttatcacct
     8581 cagtggatcc aaaggcaaga cggagtattt ctgcatctag aagacgacct aggattggct
     8641 aagactgcag tccaaatacc acaaaatcta actccgatac ttctgctatg tgacggtacg
     8701 agaacgctct cgtctattaa tggagggtta ttactgcaag gtatctcaat aggtgaagaa
     8761 agaatttcta accttattca gcagttagat gatgctttgc tattagaaaa cggtaagttc
     8821 gccggagaaa aagacaaggc tattcataaa tatagaaatt ccaaacatcg cccaatgtcc
     8881 ttcgccggaa caatatatcc ggctataact tctgatctgg acctttttat tgaagagagc
     8941 aagtccaaat ttgggccgtc tggccaatcc aaccaatatg acgggaccct gcaaggagtg
     9001 ctatctccac atattgattt cgcccgaggt tatgctactt acgcgaaatt gtggagggag
     9061 agtgaacagg atctggacgg cattgaacag gtggtcattt taggtacgga tcacagcggg
     9121 gggttaggct ccataacccc aactatgcaa aattaccaaa ctccatactc agagctagaa
     9181 acagacctag agggggtaag gttgatagag gcggctattg taggaaaaga tccttacaat
     9241 gaggagctgc atcacattaa agaacattcc atagaactag ccctgacatg gcttcataat
     9301 tccatgggag gcaaacagtt caaattgttg cctatattat gtggttcctt tcagaagttt
     9361 attattgaca aacaacaacc tgacagcgat gaaacaatta aaaatataat tgattctctc
     9421 tttgaatatc gcaaaagagc gcgaacttta gtgatagcgg caggagacct agcacacgtt
     9481 gggccggagt ttggcgatgc cacctcaatg gatgaattat ctcttggaga tctagcctca
     9541 aaagaccata tttctttagc aaatattgaa aaaggagacc ctgacagttt ttttcaagcc
     9601 agtgcggaag aaggagatgt aagaaaaatt tgtggcctta ctcccatata cctaatgggg
     9661 aaagttttga gtgactgtaa gggatacagt gtcggatatg atcaatgtcc agcagatcaa
     9721 aatagaacct ctgttgtgtc tattgcaggc actttgttat tcgggcactg aggcaaagta
     9781 gatatgaaaa gggtagggtt tttactaaaa gtacgagaaa aatatatcga agaatacaag
     9841 acccaccacc aaaatgtctg ggaggaaatg aagagcgcat tggtcagaca tggatggaat
     9901 aactattcct tattcattag accggatggg cttttatttg gttattttga agcaaatgaa
     9961 tctttctcag attccctgac gggaatgtct aaggagcaaa ttaacacaga atggcaagaa
    10021 ctaatgtcgc catattttga aattcttgaa ggctcggacc ctgaccagtc tatcgtggag
    10081 cttgaggaag tttttcatct cgattaagga gtaaaaacat aagatgagac taaagtcgaa
    10141 aaaagtcatc gttactggag gagcttcagg aataggaagg gcgatatgta ctgtcttcgc
    10201 agcagaaggc gcatcggtag tgatagccga catcgatgaa cccggctcta tggaaacttt
    10261 atctctgata cagtcagcag gaggacatgc tgcagctatt accacagacg tatcatcaga
    10321 agaaagcgta gctaaaatga tatcggcctc ggaatcattc ctaggtggga ttgatatact
    10381 cgtaaacgat gctgctgcat ttgtatttgg caaattggaa gatgttagcc gtaaagattg
    10441 ggaagtggtg tttggtgtca atgtgattgg agcctcaaat actgtaaagc actcccttcc
    10501 atttctaaaa aaatctgcgt ccccaaacat cgttaatatt gcgtctgtca gtggttttat
    10561 agctcagccg gcatttgtgc cttacaacgc ttcaaaagga gccttactac agctgaccaa
    10621 atgtatggcg atggaccttt cagaattcaa gataagggtt aacagtgttt gtcccggggc
    10681 catctatacc cctgctactg aacggcatat agccttcgaa aatgcagaca gggatgaatt
    10741 cttaaaaaag gcaggcgaag gaagcttcct taagagagtt ggccgaccag aggaggttgc
    10801 atatgcagcg ctattccttg cgtcagatga agcatctttc atcaccggca cccagctcgt
    10861 cgtggacgga ggggccacag tatgagttcg actggatttg aattcgtaga tgctcacgtg
    10921 catttttacg atatgaaaca tcccacacta cgttatgggc attggcaacc agatgaagac
    10981 catccctttt tgggatcgca aacccgtata ttgggctcca aaaattatct agccgaaaac
    11041 tttctggaag attctcaact gcacggggtg actaaagcgg tacatgtgca agccgctata
    11101 ggttctaaag atcccgtcga agaaactaaa tggttgcaag aagtttatca aaacacaggg
    11161 ttcccaaacg ccatagttgg acatgtaaac ctaagaggta gtgaagcagg ccatgtaata
    11221 gaacgtcatc gtcagcatcg tcattatcac cacccttatc gtcgtcatca tcatcagcgt
    11281 catcgtcatc atcatcatca tcaccatcgt aataatctg
//
LOCUS       DASCNS010000042        22533 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000366051, whole genome shotgun sequence.
ACCESSION   DASCNS010000042 DASCNS010000000
VERSION     DASCNS010000042.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 22533)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22533)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22533
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000366051"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="QF838_01650"
     CDS             complement(<1..875)
                     /locus_tag="QF838_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HJO59430.1"
                     /translation="MSLLVVGSVAYDSVTTAAGSRADSLGGSALYFSASASYFTNVSL
                     VAVVGEDFSKADRKKLSAREVDISGLETAKGKTFRWEGIYDTENLNFRETIDTQLNVF
                     ADFSPSLAKNNMSHPCLFLANIDPTLQHEVLTQMNPKPRIVALDTMDYWINDHKDNLS
                     KTIENIDVLFMDETEIKSFTSKSNIFSAAQAVRDMGPRIVLVKRGEYGAVMFNEGNIF
                     SVPAFPVESVIDPTGAGDSFAGGFMGYLSNMNDFSHETLRRAAVIASVMGSFAVEGFS
                     TDRVGNLNRLEIDDR"
     gene            complement(879..1232)
                     /locus_tag="QF838_01655"
     CDS             complement(879..1232)
                     /locus_tag="QF838_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HJO59431.1"
                     /translation="MPKQIEGEPYVRISVDEAADLHGNEDVVFVDVRRTDEYIEGHVE
                     GALFITVDDVLGRIDELPRDKDLLFICAAGVRSGLACEMAAAMGYDSAKLFNIEEGTP
                     SWIERGYPTSQGTDL"
     gene            complement(1250..2113)
                     /gene="mtnP"
                     /locus_tag="QF838_01660"
     CDS             complement(1250..2113)
                     /gene="mtnP"
                     /locus_tag="QF838_01660"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HJO59432.1"
                     /translation="MIEAAFIGGSGVYELEDLRDIETIEIITPFGNTSAAVTLGSIGH
                     KRVAFIPRHGTDHSLSPSEVPYKANIYALKALGVRKVISVSAVGSLNEAIKPLDIVIP
                     DQLIDRTKSREDTFFGDGLVAHISFANPFCKNLSSMIDSFCESLAIDRHLSGTYLAIE
                     GPQFSTKAESNLYRKWGCDIIGMTAIPEAKLAREAEMCYATIAVVTDYDCWKEDQEEV
                     TASMVINNLKQNVETSKKLISKIAGNINTLGDECLCNASLRDALVTQRILDNQKIAKK
                     LGVLLQKYLPA"
     regulatory      complement(2197..2328)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2382..3578)
                     /locus_tag="QF838_01665"
     CDS             complement(2382..3578)
                     /locus_tag="QF838_01665"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HJO59433.1"
                     /translation="MTISSGNILLVQSGGCTPVMNSTLAGLIKNGQTLYPQSRIYGGI
                     HGIEGIIDGKIPDITELSNSQLKSLSKMPGAALRSSRHKLLPNDIGPLLNTLRAHDIR
                     ILHTIGGNDSAVTGLRVSEAAQEDKYELQVINLPKTIDNDIVLTDHSPGYGSAARFIS
                     QATFGIGKDAEAMGINSPICILEVMGRDSGWLAAASSFYKKTDHDAPHFIGIPEVIFS
                     EEHFMISIESAYRNYGYAIAVVSEKIRSAEGNLGRDNPPTFTDNFGHNYFESPGKYLS
                     NLVEKRLGVRCRWEKPGTIQRSFMDAVSTTDREEAYQIGQKAVEMARSGMNDVIPTLI
                     RSNNQKYEYSIGSASLKDVAGEIRYLPEKYRPDEQGYTSDSFKSYLRPLLGNPIHDTD
                     SIFHLL"
     gene            complement(3599..4333)
                     /locus_tag="QF838_01670"
     CDS             complement(3599..4333)
                     /locus_tag="QF838_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HJO59434.1"
                     /translation="MRGKNLAQVKAMTFDVFGTVVDWRSSIAREIQNVGKTKGFDLDW
                     NSFADEWRSGYAPSMNKVRAGELPWTKIDNLHRMILDELLSKHKISNLSEEETDGLNR
                     AWHRLDPWPDSVEGLTLLKKDYIISSLSNGNVSLLVNMAKYGGLPWDTVLSAEIAKHY
                     KPDPESYLSTSEYLGLPINQVMMVAAHKNDLKAAKSTGMITGYVPRPKEHGENTIVDY
                     AEEEYVDIIADDFIDLSKKMSAIKEV"
     gene            complement(4354..5847)
                     /locus_tag="QF838_01675"
     CDS             complement(4354..5847)
                     /locus_tag="QF838_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HJO59435.1"
                     /translation="MPTLADLTTNHPKNKNAILIPGGPTTTYGEFNDQIEKIAEILSA
                     AGVEKGRTVSIVLGNGLDFMITFLAVTRSGAIAAPLNSAYTNEEFKFYMEDTESQLVI
                     IAPGADVAKQAADELNIPVAEVNLDNRGQLSVSKSGSLLTARSPVSAPSEDDIALFLH
                     TSGTTSRPKGVPLTHGNLIASLRNIVNTYDLTEDDLAMVVMPLFHVHGLIGVSLSTMK
                     SGGSMVIPPRFSASNFWQIQATTNATWYSAVPTIHQILLMRADEDKAPSQSFRFIRSC
                     SAALAPTVFNDLENRFGAPVLEAYGMTEASHQMSSNLLPPGPRNPGTVGKGTGVDIGI
                     MDENGTVLATNQIGEVVIRGSNVTHGYHNNPSANEEAFTNGWFRTGDQGILSEESILT
                     LTGRLKELINRGGEKISPLEVDATILQHPNVSEAVCFGVPDEKYGEAVQAAVVVSSGT
                     SESEIRSFCSQHLADFKIPDRVYIVEELPRTATGKIQRRHVAAKFAE"
     gene            complement(5913..6899)
                     /gene="galE"
                     /locus_tag="QF838_01680"
     CDS             complement(5913..6899)
                     /gene="galE"
                     /locus_tag="QF838_01680"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HJO59436.1"
                     /translation="MKILITGGAGYIGSMVCHSLADHGHEPIIIDSMITGSGQQVDQF
                     PTFTGDIANDDLIKNIIRKHPEIESVVHCAGKILVPESAVHPDQYYYENVSKSLHLFT
                     TLKTVGIKKILFSSSASIYKSQNGGSVDEDSILTPLSPYAKTKFMTELILEDFCNAFE
                     IEGIALRYFNPIGADPKMRCGPSLNNTSHLLGNLVRAAEGFTNNIKITGSDWPTKDGT
                     GVRDYIHVWDLAEAHVAALESFHSILASKSKFEKINIGGGSPTTVKEFIKIFEEVYGC
                     EIATDIAPPRDGDTAGAFANIGKAEKLMNWRPRMSVEQGIADTIKWHRKMAD"
     gene            7051..7665
                     /locus_tag="QF838_01685"
     CDS             7051..7665
                     /locus_tag="QF838_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019590.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HJO59437.1"
                     /translation="MIYEFRTYDIKPRTVDQFGENTKAKLNKRLEYSPLGGFWYTEMG
                     PLNQVVHIWPYEDLNHRSEIRSKAVSDGNWPPDNNDIILNMESEIMLPAPFMRPLEAK
                     TIGPIYEMRIYTYKPGDIPKVLDAWGAAIQKREEYSPLVGCWYSDIGGLNKFIHMWSY
                     DSFERRTSVRTETRAQGVWPPKGGVAPVKQENKILLPFDFSPLQ"
     gene            complement(7674..8792)
                     /locus_tag="QF838_01690"
     CDS             complement(7674..8792)
                     /locus_tag="QF838_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJO59438.1"
                     /translation="MQSNVCVVGGGIVGLSSAYFLAKTGHSVTVIERDSIGSHASGFA
                     YGSLSPLGEAGQADDILPELEIARMGMDIHTQFSKELFESTGINTDHRFRPALDLIFT
                     ASEASEAKNQVEWRSDEKGYRVEWLRGEEALKLVPHISKEVLGAVYTEGVADVEPYKL
                     LLALTQACELLGVNIVHGDVSSVIREGSAISITTQSGVRTCENLVLAMGPWMGKTAEW
                     LGINVPITPLKGQIVRLNAPNVTVSCSVGWKGNYACTKPDGLLWAGTTEENVGFNDIP
                     TTGGRDQVLEALNKMIPNLDDATLVQHTACLRPLPVDGKIIIGSVPNDQNIFLATGTG
                     RKGILLGPAMGKIISQLISGQNPEVEITSFSPARFASF"
     gene            complement(8802..10178)
                     /locus_tag="QF838_01695"
     CDS             complement(8802..10178)
                     /locus_tag="QF838_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HJO59439.1"
                     /translation="MATTTTLSGEVIEELKQQAAEHFWPHARQTNDMFGDNGLKLVSD
                     SKGVWVYDVDGNEWFDTLSGMWLVNIGHGRKEIADAVYEQMQGISYSPGGTLTPVTAE
                     LAGRVAALAPDKQARVYFVSGGSEAVETALKMAKNYQKNIGEGTRYKVISRKGSYHGA
                     THACMSLGGGGVAAPINYGPLMPGNIHIEQPDAYRGRCCSADGGCTLECAKDLERAIL
                     HEGPSTVAAFIGEPISAASGIHIPHPEYWPTIREICDKYGVVMICDEVINAFGRTGKM
                     FATEHWGINPDITTVAKALTSGYLPIGAAIASKKIASAFEGGDDDTFRHLITFGGNPA
                     SCAAAIANLDIMENENIVENAAAMGDYMYERLQSLYDHKIVGDVRGGKGLLSAVELVK
                     DRDTREKFPAELKLADKVTAALQKHMILGRAGDSIPIAPPLCITKNEVDHFVGQLNSA
                     LTEIETTL"
     gene            complement(10206..10352)
                     /locus_tag="QF838_01700"
     CDS             complement(10206..10352)
                     /locus_tag="QF838_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HJO59440.1"
                     /translation="MLEAYCLKCKGPHEIKEPNEITLKNGRAATQGKCGACGSKVFRI
                     GKAS"
     gene            10595..11362
                     /locus_tag="QF838_01705"
     CDS             10595..11362
                     /locus_tag="QF838_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013530.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO59441.1"
                     /translation="MCKKKVLITGVSGLVGSVLAKSLDQDYEITGVDLKECSQVPTVA
                     ADCSILQDILPVFSGIDTVIDLASNPSQYSAWDVIHNVNLKCTSNTLEASKIAGVKRV
                     IFASSNHATGMYEFDSPYSEIVKGSYSGLTADSIPLITTDMPVRPDGPYGIAKAFGEA
                     AGRFYSDQYGISSISVRIGTLNSEGRPTNQRQFATLFSHSDMSNLFGKCIEAPSSLKY
                     GIYYGVSNNKWRFWDISNSERDLGYAPIDDAEIWRNG"
     gene            11438..11932
                     /locus_tag="QF838_01710"
     CDS             11438..11932
                     /locus_tag="QF838_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO59442.1"
                     /translation="MEQVLTPPPIIEQFKKNAVTHVIWLPDSETNFMYESMTREEKLK
                     IVPVCREGESMAIAAGLWVGGKKPVVLIQNTGMFEAGDSIRGLGIDIEFPLVMMIGYR
                     GWTRHGITKDSAAHFTEPILNAYGINYYLIESNDDVDRISSAFEEAERTRRPVACLLG
                     AEYS"
     gene            complement(11940..12392)
                     /gene="rpiB"
                     /locus_tag="QF838_01715"
     CDS             complement(11940..12392)
                     /gene="rpiB"
                     /locus_tag="QF838_01715"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HJO59443.1"
                     /translation="MRISVAADHNGFELKNEILAILKKSGHDVIDIGPHFLDPLDDYP
                     DYAKPLAESVSSGKTDRGIMVCGSGVGASVAANKVKGVRAAVCHDIYSAHQGVEHDDM
                     NVLCLGSRIVGAEVVRELVSAFISAVYTNEERHARRLNKVIEMENDFK"
     gene            12526..14508
                     /locus_tag="QF838_01720"
     CDS             12526..14508
                     /locus_tag="QF838_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HJO59444.1"
                     /translation="MSVSRFEITSKVLLEDGKEYGDIGTYDHMQGTVYFEIDPLLESN
                     ERIVDIQLAPRNPDGKVEFSADFVLLTPSDPDRGNGTMFLDVVNRGNKTVLYGFNSSN
                     RPTDPTSPIESGNGFLMREGYAVMFCGWQADIPDIPGLIGLSVPEAYVDGEQLSGKVM
                     NQYQANVATSVFPLADRYHLKNPAADETDPKAELMVQDQPNGIAELIERDKWALVRVE
                     DSEIEPDVSHVHLQGGFELGRIYKLVYTAKGSRIVGLGFAAVRDICSFIKYASEDEGN
                     PLSGYVDHAISYGVSQTGRFLRQYIYTGMNVDESSRQSMDGIIAHVGGGMRGEFNLRF
                     GQPSKDVCYIIPELFPFTDTEQKDAVTGREDGLLDRMVSQGNVPKIMFTNSSAEYWRG
                     DAALIHTNIENNSDAPEHPNVRRYHFSGTQHGSGKYPLETVRESDSVRGNFPFNGVNY
                     TPILRGCLTNMNNWVKGENDPPPSSHPKHEDGSAIETKDVIGKFSKIPGIRLPDRVLN
                     PMRLDYGDEQHLGRTVKLPPEQGETYPAFVSDVDETLNEIAGIRLPDISVPVATNTGW
                     NTRHSLIGNEGLLIGITGGLAGWTVPLPSTEGEKEKDHDPRPSIESLYSNKQNYMSQI
                     QEAAQKLIEEGYILSEDFQGVVDICEQKYDDITGAE"
     gene            14536..15279
                     /locus_tag="QF838_01725"
     CDS             14536..15279
                     /locus_tag="QF838_01725"
                     /inference="COORDINATES: protein motif:HMM:NF037430.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HJO59445.1"
                     /translation="MQIVQTSDNWLSKEAVFKHLNELAENTYTDRTVYLAANFKESKS
                     VTPKIAGPVIEAVINEVGSNDTGMVIFRRDEELTVIEPPLPFTMDAIMQDQDTLLLED
                     IFDVPKTVAVVLVRLGRYAVALMEGQKLVDTKTEGRQMKNRHKAGGSSQRRFERSRER
                     LIRELYDKVCEVSQRIFEPRINDIDYLFLGGEKHTVNGFKKRCGFIGKFDGKVMSRLI
                     NIDEPNSEALKSVGDEIYKSRVRVFKTVR"
     gene            complement(15288..16061)
                     /locus_tag="QF838_01730"
     CDS             complement(15288..16061)
                     /locus_tag="QF838_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59446.1"
                     /translation="MCAKKNVGRLSGKVALITGAKSGIGEACVERFVEEGARVVAADI
                     SVPDSYVGDTGSILKISGDVTKSGDAAEMVNRTIQTYGKLDILVNSAGISSRNAFEED
                     ADPEKVWDRVIEVNLKGTYLVSRYAVPEMELNGSGSIINLASINGLVGYPVGIGGGFN
                     AYPPSKGGVIQFTKTLANDMASKNVRVNCLCPGYVETNLTESLTNNEPIYQQLKNLHP
                     MGRLGRPEEIANAALFLASDEASFITGSSLVIDGGYTSQ"
     gene            16185..16529
                     /locus_tag="QF838_01735"
     CDS             16185..16529
                     /locus_tag="QF838_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HJO59447.1"
                     /translation="MPEFKNHVLVCDVERDDGIHCGNKGGAEVKRKVVEMLSKYGLRK
                     SVYVSSTGCTSQHRKCETDQCSMIVYGPGNEGTWYIANPDDVETIVKEHLVDGNTVDA
                     LVNDGMKINLDA"
     gene            complement(16538..17197)
                     /locus_tag="QF838_01740"
     CDS             complement(16538..17197)
                     /locus_tag="QF838_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HJO59448.1"
                     /translation="MNNILLVGGGEFQEECLAMDSYLVESLNKKHPRVAIIPTAAANQ
                     RPEKAAENGVRHFDALGANVNPIMILNKAHAEDEIYCNDLNGVDLIYLTGGDPKHLLN
                     TLYQTPFMASITEAVSKGATLAGSSAGAMVLGSKMRYRSWMPAIGLISNTAVLPHHEN
                     SVPETVSSENQSELQSGMEIIGIDSATGVFLGDNGVKIKGKGKAVFYSRSTFKIIESA
                     S"
     gene            complement(17219..17686)
                     /locus_tag="QF838_01745"
     CDS             complement(17219..17686)
                     /locus_tag="QF838_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HJO59449.1"
                     /translation="MGTIEDRIQELGIELPDSPAPMANYVPVVRTGNLIYLSGVGPTP
                     KPDGSAYTGKLGGALGVEEGYNVARLTAINLVARLKGYLGELDRVNQIVKLLSMVNSV
                     PDFTEPPAVTNGCSDLLVEIFGDKGRHARSAVGVATLPGGMPIEIEMIAEISD"
     gene            17783..18178
                     /locus_tag="QF838_01750"
     CDS             17783..18178
                     /locus_tag="QF838_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJO59450.1"
                     /translation="MSLTADHVRLKAVNHVTYNVVDKERATKFWVDVLGVQQIPKQVD
                     AEHIIWLQLPSGAMVHIVETPDGPSTPSHHGAFEVDDIDAAANVVQQKGIETTDITTR
                     NDGQRVFFLNDPEGNRIEICTKSGFGVLV"
     gene            complement(18182..19234)
                     /locus_tag="QF838_01755"
     CDS             complement(18182..19234)
                     /locus_tag="QF838_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HJO59451.1"
                     /translation="MSDPTQKGKIQSVLGLIDPADLGLTMTHEHLLIDFSVMFNPSPD
                     VTTQRLAHIPVSMENLGWIRQYSYSNLDNLLVLDEDTAIEEATLYQRHGGGAIVDATT
                     VGIGRDPLALARISRGAGLHVVMGAGYYVDAAQPNRINEMDENDIYQEIVQDIQIGVG
                     STGIKAGIIGEIGCTWPLMANELKVLKASARAQSETGASILIHPGRDENAPIEILNIL
                     IDSGADISRVIMGHLDRTVDTIEMLGKIADTGCVLEWDLFGNEVSFYQPSDFDMPSDA
                     QRLNFIRQMIDNGLGDRIVISHDICTKHRLVKYGGHGYGYIPEHIIPRMRNRNFHESE
                     IEAITKNTPARLLTIV"
     gene            complement(19547..20830)
                     /locus_tag="QF838_01760"
     CDS             complement(19547..20830)
                     /locus_tag="QF838_01760"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59452.1"
                     /translation="MAQSVKKPNIPCEINSDQTPSKKRRFSNILDALSYRDFRWLWIG
                     SFVSFMAMNMQMITRSWLILRVTDDSPLALTLVTLTFALPMTVVAPFAGALADRFSRK
                     KLIVFSQIGSGVFTLILGTLDLIGIVTFWHIMVIGIFNGSLMSINMPSRQAIISDIVP
                     DSKLMNAISLSNSSMNLTRVAGPALAGFLILLLDTAGVFFVISIIYVFAAVTIGMVHY
                     APNRSARTGKNVGGDVADGIRYAFANPALRGLFIMSFIPVLFGFSYMALVPAWAREAL
                     AIESDGLGILLMLMGIGATIGTLLLSALGNIPRRGVVMLCASLAWGVALAIFAQVTSF
                     SMAIPFLIFIGLTSSMYMSLNMTMVQLKADPEMRGRAMSITMMTFGMMPLSAVPFGTL
                     AEKIGTADSLTISGILLALFTIGFAMSNKTFRKMD"
     gene            20977..22155
                     /locus_tag="QF838_01765"
     CDS             20977..22155
                     /locus_tag="QF838_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59453.1"
                     /translation="MASESSQSLWSRDFVINLLVAHLLLAGFFSLFMVVPGFIDKLGG
                     KEWEIGVIIATFGLAGVIFRPMAGKWILKSGPKRVIMFGILIFVGTTVLYIPLPSEWW
                     IVPVRMAQGVGLAIAPVATSTVVANLAPESRRGEGMSYMGNSISIAQLYAPPIGFFIA
                     DSFSFSLAFIVASAVALVSFFVCLTMSDSRTRMPITPQNENEMSDAPFISRAAIFPTL
                     IFLTYAFTMAPVSTFLPMLSDVRELGVNPGFYFTILSGMTMLTTLVSGRIADKFGRSA
                     VIIPGLTLVSVAMFMLSGSFTSGMFFGAAFFHGVGYGLIYPGINSLVIDRVPANERGS
                     AIGTLQQAWDIGASGGNFIVGPIIGALGVGSAFVVVGAGVATGVVGFITGNRKSKNFS
                     "
     gene            22240..>22533
                     /locus_tag="QF838_01770"
     CDS             22240..>22533
                     /locus_tag="QF838_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO59454.1"
                     /translation="MNGEQLRKKTSSGGIAYGTMLSMGRNPRWSQAISSFGLDYVIID
                     TEHAPRSRAEVADFLAAFTYSGVVPIVRIPIPASHYVTMYIDAGAQGVLAPYCE"
BASE COUNT         6130 a         4725 c         4845 g         6833 t
ORIGIN      
        1 aatctatcgt caatttccaa acggtttagg ttaccgaccc gatctgtgct aaagccttca
       61 actgcgaatg atcccataac agatgcgatc acagcagctc ttctgagggt ttcatgtgaa
      121 aaatcgttca tattggataa atatcccatg aatccaccag caaaagagtc acctgcacca
      181 gtggggtcta tgacactttc cacaggaaag gcaggtactg agaatatatt tccttcatta
      241 aacattacgg ccccgtattc acctcttttc acaaggacta tacgtggtcc catatcccga
      301 acggcttggg ccgcagaaaa tatgttggat tttgaagtga aagatttaat ctctgtttca
      361 tccataaaaa gcacatcgat gttttctatt gttttgctca aattatcttt atggtcattg
      421 atccaataat ccatggtgtc cagtgccaca atcctcggtt taggattcat ctgtgttagc
      481 acctcatgtt gtaatgtagg atcaatattt gcgagaaata agcatgggtg cgacatgtta
      541 ttttttgcca atgagggaga aaaatcggcg aatacattca actgggtatc tattgtctcg
      601 cggaagttta ggttttccgt gtcataaatt ccttcccatc gaaaagtttt gccttttgct
      661 gtttcaagac cgcttatatc gacttcccta gcactcaatt tttttctatc ggcttttgag
      721 aagtcttctc ctacaactgc taccaaacta acatttgtaa aatagcttgc tgaagcgctg
      781 aaatacaacg cggagccacc cagagagtct gctcgcgatc ctgcggcagt tgttactgaa
      841 tcatacgcaa cggacccgac aactaataaa gacatagact ataaatcagt accttggcta
      901 gtcgggtaac cacgttcaat ccatgatggt gtgccttcct caatgttgaa taacttcgct
      961 gagtcatatc ccattgcagc ggccatttca caagcaagac cactcctgac cccagctgcg
     1021 caaatgaaca agagatcttt gtctctagga agctcatcta tgcgtccgag aacatcatct
     1081 accgtgatga acaaggctcc ctctacatga ccttcaatgt attcatcggt tcttcgtacg
     1141 tccacaaaaa ccacatcttc attaccgtgt agatcggccg cttcatccac agagattcta
     1201 acgtaaggtt ccccttcgat ttgtttaggc attacacact ccttcgattt caggctggta
     1261 aatatttttg taacaacaca cctagttttt ttgcgatttt ttggttgtct aggattcttt
     1321 gcgttaccag tgcgtctcgc aaagatgcat tgcataagca ctcatctccc aatgtattaa
     1381 tatttcctgc tattttactg attagttttt ttgaagtctc cacattttgt ttgagattat
     1441 taattaccat gctagcggtt acttcttctt gatcttcctt ccaacagtca taatctgtta
     1501 caacagctat tgttgcgtaa cacatctccg cctcccgagc taatttggcc tctgggatag
     1561 cagtcatccc tatgatgtcg caaccccatt ttctatataa attggactca gctttggtgg
     1621 aaaactgagg gccttctata gccaggtatg taccagataa atgtcgatct attgctaggc
     1681 tttcacaaaa agaatcaatc attgaggaaa ggtttttgca aaatggattt gcaaaactta
     1741 tgtgggctac gagaccatct ccaaagaaag tgtcttctct ggatttggtt cggtctatca
     1801 gttggtccgg aattactata tctaacggtt tgatggcttc attcaagctt cctactgcgg
     1861 acactgatat aacttttctg acccccaaag cctttaaagc ataaatgtta gctttgtaag
     1921 ggacttctga aggggataaa gaatgatcag ttccatgcct tggtatgaac gccactcttt
     1981 tgtgtccaat tgaccctaat gtaactgcgg cacttgtatt accaaaaggg gtgatgattt
     2041 caatggtttc gatgtctctg aggtcttcca actcatagac tccggatcca ccgatgaaag
     2101 cagcttctat catattgtta atttgagttc cgataatttt tcaccaaaaa aaaacttccc
     2161 aaagagagaa gttactgaaa ataattattg tatgttctct catcttccgg gcattatatt
     2221 atcccgctgg aattggcacc gtatttcaaa taagagtgtt tattgatctt catcaaagtc
     2281 ggttgccggg cgtctaaggg ccagtccctc agccactcta gataagttcg ctatatttag
     2341 ttgtggaaaa atcgtacctt agtgcaatag ttgagtcaat ttcaaagtaa gtgaaagatg
     2401 gagtcagtgt cgtgtattgg atttcctaat aaaggcctga ggtaagactt aaatgaatcg
     2461 ctcgtataac cttgttcgtc ggggcggtat ttttcaggta aatatcttat ttctccggct
     2521 acatctttta ggctagccga gcctattgag tattcgtatt tttgattatt ggaacggatt
     2581 aaagtaggga tcacgtcatt cattccagat cgggccattt caactgcctt ttggcctatt
     2641 tgataggcct cttcgcgatc ggttgtagac actgcgtcca taaaggacct ctggatagtt
     2701 cctggttttt cccatctaca cctgactccc aatctttttt caacaagatt tgacaaatat
     2761 ttgccagggc tttcaaaata attatgtccg aaattgtcag tgaaggttgg aggattgtcc
     2821 cgacctaaat tgccctctgc ggaccttatt ttctctgaaa ctactgctat tgcatacccg
     2881 taatttctat atgcactttc aatggaaatc atgaagtgtt cctctgaaaa tataacttct
     2941 ggtattccga tgaaatgtgg ggcgtcatgg tcagttttct tatagaagga actagctgct
     3001 gccaaccatc cagaatctct acccataact tcaagaatac atatcggtga gtttattccc
     3061 atagcttctg cgtctttacc gataccaaaa gtggcttgac ttatgaaacg agcggcgctt
     3121 ccataaccgg gggaatgatc tgtaaggact atgtcgttgt ctatcgtttt tggaaggtta
     3181 atgacttgta gttcatattt atcctcttgg gctgcctctg aaacccttaa tcctgttacg
     3241 gctgaatcat ttccaccgat tgtgtggaga attcttatgt cgtgagccct taatgtattt
     3301 aataaaggtc caatatcatt agggagtaac ttatgcctgc tacttcttaa agcagccccc
     3361 ggcatttttg atagagattt taactgggag ttagataatt ctgtaatatc tggaatttta
     3421 ccgtctataa ttccctcgat tccgtgaatc ccaccgtaaa ttcggctctg tggataaagg
     3481 gtttgcccat tctttatgag tccagctaga gtactgttca tgactggggt gcaccctcca
     3541 gactggacaa gcaggatatt tccagatgaa atggtcatgt ggttcttatg actatcgctc
     3601 agacttcttt tatggcagac atttttttag ataaatctat aaaatcgtcg gctattatgt
     3661 ctacgtattc ctcttcggcg taatcgacaa tcgtattttc tccgtgttct ttgggtcggg
     3721 ggacatatcc tgttatcatt ccagtgcttt tggctgcctt cagatcattt ttgtgggctg
     3781 caaccatcat cacttgattt attgggagac caagatattc acttgtgctc aaataagatt
     3841 caggatctgg tttatagtgt tttgctattt cggcggaaag taccgtatcc catggcaacc
     3901 caccatattt ggccatgttt actaatagag aaacattacc gttagaaagt gagctgataa
     3961 tgtaatcttt tttcaggagt gtgagaccct caaccgagtc aggccacggg tccaatcgat
     4021 gccaggccct attcagacca tctgtttcct cttccgagag attggagatt ttatgctttg
     4081 acaataattc atccaatatc atccggtgca ggttatcaat cttagtccaa ggcagttctc
     4141 cggccctaac cttgttcatt gagggagcgt atcctgacct ccattcatca gcgaacgaat
     4201 tccagtctaa gtcgaatccc tttgttttac ccacattttg tatttctctg gctatactac
     4261 ttcgccaatc aacaacagtg ccgaaaacgt caaaagtcat agcttttact tgagctaggt
     4321 ttttccccct cattaaattc accttgtcta attttattct gcaaacttgg cggctacatg
     4381 tcttcgctga atcttccctg tagctgttct gggtagttcc tcgactatat agacacggtc
     4441 tgggattttg aaatcggcca aatgctggct acagaaagag cgtatctcac tttcacttgt
     4501 ccctgaggaa accactacag ctgcctgtac ggcttccccg tatttctcat ctggaacccc
     4561 gaaacagact gcctctgaaa catttgggtg ctgcagtatc gtcgcgtcaa cttctagtgg
     4621 ggaaattttt tcgcccccac ggttaatgag ttctttaagt cttcccgtca aagtgagtat
     4681 cgactcttcg gacagaattc cctgatcgcc ggttctaaac caaccattag tgaatgcctc
     4741 ttcattggca gaagggttat tgtgatatcc atgggtgaca ttcgaacctc ttataacaac
     4801 ctctccaatt tggtttgttg ctaggaccgt gccattttca tccataattc ctatgtcaac
     4861 accagtccct tttccgacgg tacctggatt tcttggcccg ggaggtaaga ggtttgatga
     4921 catttggtga gacgcctcag tcatgccgta ggcctctagc acgggagccc caaatctatt
     4981 ctcaagatca ttgaaaactg taggggccaa tgctgcagag caagatcgta tgaagcgaaa
     5041 tgattggcta ggtgccttgt cttcatccgc tctcatcaat agtatctggt gaatagtggg
     5101 caccgctgaa taccaagtgg catttgtggt tgcttgtatt tgccaaaaat tgctggcact
     5161 aaatcttggt gggatcacca tgctgccacc actcttcata gtggatagcg aaactcctat
     5221 aagcccatga acatggaaca acggcataac aaccattgct aaatcatctt cagtgagatc
     5281 gtaggtattt actatgtttc ttaaagatgc tatcaaattg ccgtgggtca aaggaactcc
     5341 ctttggcctg ctagtggtgc ctgatgtgtg cagaaaaaga gctatgtcat cttctgaagg
     5401 agccgatacg ggggacctcg ccgttaacaa agatcctgac tttgatactg ataattggcc
     5461 tcgattatct aaattaactt cagccaccgg tatatttaac tcatcagcgg cttgtttcgc
     5521 aacatctgca cctggagcaa taataaccaa ctgggattcg gtatcctcca tataaaattt
     5581 aaattcttcg ttggtataag ctgagttcaa gggagccgca atggcgccgg acctcgttac
     5641 tgcgagaaag gtgatcataa aatctagacc atttccgagt acgatcgaaa cggttcgacc
     5701 tttttctaca ccagcagcag aaagtatttc tgctattttt tctatctggt cgttaaactc
     5761 accgtaagtt gttgttggtc cgccaggtat tagaatggca ttcttattct ttgggtgatt
     5821 tgttgttagg tctgctaatg taggcaactt aatccctcct tgggagctag gtaacagaat
     5881 tgtactgaat gaaatgaata ccgcaacttt gtctaatccg ccatttttct gtgccatttg
     5941 atcgtgtcgg ctattccttg ctcgacagac attcttggtc gccagttcat taatttttcg
     6001 gccttcccta tattagcaaa tgcacctgca gtatccccat cacggggagg agcgatatca
     6061 gttgctattt cgcatccata tacttcctca aaaatcttga taaattcttt tacagttgta
     6121 ggactaccgc ctccaatatt aattttttcg aatttggatt ttgacgctag tattgaatgg
     6181 aaagattcta gcgctgcaac gtgggcctca gctagatccc aaacatgaat ataatcccgt
     6241 actcccgtac cgtctttggt tggccagtca gaaccggtga ttttaatgtt atttgtgaac
     6301 ccttctgcag ctcttactaa gttgcctaat aggtgagagg tgttgtttag gctgggccca
     6361 cacctcattt ttggatcagc accaataggg ttaaaatatc taagcgcaat cccttcaatt
     6421 tcaaaggcat tacaaaaatc ttccaaaatc agttcggtca tgaatttggt tttagcgtaa
     6481 ggactcaatg gagtcaaaat ggaatcctca tcaacactcc ctccattttg agacttgtag
     6541 atggaagcac tagaactaaa aagtattttt ttgataccaa cagtttttag ggttgtaaat
     6601 aaatgaagag atttgctcac attttcataa taatattgat ctgggtgcac agctgattcg
     6661 gggactaaaa ttttccctgc acaatgaacc acactctcaa tctcagggtg ttttctgatt
     6721 atatttttta ttagatcatc attggcgata tcaccagtga atgtagggaa ttgatccacc
     6781 tgttgaccgg accctgtgat catggaatca attattattg gttcatgccc atggtcagct
     6841 agagaatgac aaaccatgct tccaatgtat ccagcgccac cggttatcag tatcttcaac
     6901 tcaaatcctt tcgtgtattt tcttgagatg tgtaaattta aatatcatga actaaggcct
     6961 caaaggtact ttaaatattt tatttcattc tattatgtta ggcttgtgtt ttgagatcat
     7021 tttgagtcgg tttaggcagg agtattagtt atgatatatg agttccggac ctacgatatc
     7081 aaacccagga ccgtggatca gtttggtgaa aacacaaagg ctaaattgaa taaaaggctg
     7141 gaatactctc cgttaggagg tttttggtat accgagatgg gaccgctcaa tcaggtcgtt
     7201 catatttggc catatgaaga tttaaatcac aggtccgaaa ttaggtccaa agcagtatca
     7261 gatggaaatt ggccgccaga taataacgac atcatattaa atatggaaag tgaaataatg
     7321 cttccggcac cttttatgag gcctttagaa gctaagacta taggaccgat ttatgagatg
     7381 agaatttata cttataaacc tggagatatc cccaaagtgc tagacgcttg gggggcagct
     7441 attcaaaaaa gagaagaata ttctcctctg gttggatgtt ggtattctga cattggaggg
     7501 cttaataaat tcatacatat gtggtcatat gattcatttg agcgacggac aagcgttaga
     7561 acggagacga gggcgcaggg cgtgtggcca cctaaaggtg gagttgctcc ggtaaaacaa
     7621 gaaaataaga tactactgcc tttcgatttt tccccgctcc aataaaaaag aatctaaaag
     7681 gaagcgaatc ttgccggact aaacgaggtt atttccactt cagggttttg ccctgatatt
     7741 agctgtgaaa taattttgcc cattgctgga ccgagtagaa ttccttttct gccggtccca
     7801 gtagccaaaa aaatattttg atcgtttggt actgatccta taatgatttt cccatctaca
     7861 ggaagtggac gtagacaagc agtatgttgt actagggttg catcatccaa attagggatc
     7921 attttgttca gtgcctcaag tacttgatct ctaccacccg tagttggtat gtcgttgaaa
     7981 cctacatttt cttcagtggt tccggcccac aaaaggccat ctggttttgt gcatgcgtaa
     8041 tttcctttcc atccaactga acagctgact gtaacattag gtgcattcag tcttactatt
     8101 tggcctttta aaggagtgat gggtacattt ataccaagcc attcggctgt tttacccatc
     8161 caaggaccca tcgccagtac tagattttca caagttctga ccccactttg ggtagttatt
     8221 gaaatagcgg aaccttcacg gatgacacta cttacgtcgc cgtggacaat gttgactcca
     8281 agtaattcgc atgcttgtgt taaagccaat aatagtttgt aaggttctac gtcggctacc
     8341 ccctctgtat agacagcccc gaggacctct tttgaaatat gtgggactag cttcaaagct
     8401 tcctcacccc tgagccattc aacacggtag cctttttcgt cgcttctcca ttcaacttga
     8461 tttttcgctt cgctagcttc agatgcagtg aaaattaagt ctaaggctgg gcgaaaccta
     8521 tggtctgtgt ttattcccgt actttcaaat aattcttttg aaaattgggt gtgtatgtcc
     8581 atgcccattc tggctatttc tagctctggc aaaatatcat cagcctgacc agcttcacct
     8641 agtgggctta gacttccata ggcaaatcca gaagcatggc tgccaatgga atccctttcg
     8701 atcactgtga ctgaatgacc tgtttttgcg agaaaatagg cggaggacag tcctactata
     8761 cctccaccta cgacacaaac gtttgattgc attgcggaga tctataaggt ggtttctatt
     8821 tccgtcaaag ccgagttgag ttgacccaca aagtgatcga cttcattttt cgttatgcaa
     8881 agagggggtg cgattgggat agaatctccc gcacgtccca gtatcatatg tttttgcaat
     8941 gctgcagtga ctttgtcagc taattttaat tcagctggaa atttttctct cgtatcccgg
     9001 tcttttacaa gttctacagc actcagtagt ccttttcctc cacgaacatc cccaactatt
     9061 ttgtgatcat acagtgattg cagtcgttca tacatatagt ctcccatggc agctgcattt
     9121 tccacgatat tttcattttc catgatatct agattcgcga tggctgctgc acatgatgct
     9181 ggatttccac caaatgttat taagtgcctg aatgtgtcgt catcacctcc ctcaaaagcc
     9241 gaggcaatct ttttactggc gatagctgcg ccaataggca ggtatccact agttagtgct
     9301 ttggcgacag tagtaatatc agggttaata ccccaatgct ccgtagcgaa catttttcca
     9361 gtcctgccga aagcatttat aacttcgtcg catatcatca cgacaccgta tttatcgcat
     9421 atttctcgaa ttgtaggcca atactctggg tgaggtatgt ggatacctga tgctgccgaa
     9481 attggttcgc caatgaaggc agctacggtc gacggtccct catgaagtat ggctcgttcc
     9541 aagtcttttg cgcattctag tgtgcatccc ccatcagcac tacagcatct ccctcggtat
     9601 gcatcgggtt gttcgatatg aatgtttcct ggcattagag gaccataatt aataggagca
     9661 gcaacacctc ccccgcctaa actcatgcag gcatgagttg ctccgtggta ggagcctttt
     9721 ctgcttataa ccttgtacct ggtaccttcc cctatattct tctggtaatt cttcgccatt
     9781 ttgagtgcag tctcgactgc ctctgatcca ccgctgacaa aatacactct tgcctgtttg
     9841 tccggtgcaa gagcggcaac tcttcctgcg agttctgcgg ttaccggggt caaagtacca
     9901 ccgggggaat aagaaatccc ttgcatttgt tcataaaccg catcggcaat ttcctttctg
     9961 ccatgtccga tatttaccag ccacatacca gatagggtgt caaaccattc gttcccgtca
    10021 acgtcgtaaa cccatacccc tttcgagtca cttacaagct tgagtccatt atcaccaaac
    10081 atatcgttgg tttggcgggc gtgaggccaa aaatgctcag cagcctgctg ttttagctct
    10141 tcgattacct ctccagacag tgttgttgta gttgccatca gttcacctca tttctcaaac
    10201 tatcactaac tagctttccc aattctaaat acttttgaac cacaagctcc acatttgccc
    10261 tgagtggctg ctcttccatt ttttaatgtg atctcattag gttctttgat ttcatgcggt
    10321 cctttgcatt taaggcagta agcttccaaa atgttgcacc tcgtaatttt aggtaatttc
    10381 ccgttctttt agcttacctt tttttacgta aggatgctaa aaatcagaaa gtttcggaat
    10441 tatatctatc attaacttat ccgcaaaccc cctaaacacg acatcagaga tttagagcag
    10501 ccgaaatgag gtgccagcag ctattgtggc aattaaattt tcctgataaa aagccagtag
    10561 attttctaaa tccatttcat tgaaggtatg caatatgtgt aaaaaaaaag ttttaattac
    10621 tggggtatca ggattggtag ggagtgtgtt ggcaaaatct ctagaccaag actatgagat
    10681 cacgggagtc gacttgaagg aatgtagcca agtcccaacc gtggcggctg actgctctat
    10741 cctccaggat attttgccag ttttttccgg tatagacact gtcatcgatc ttgcaagtaa
    10801 tcccagccaa tacagtgctt gggatgttat tcataatgtc aatttaaaat gcacttccaa
    10861 tactttggag gcttctaaaa ttgcgggagt gaaaagggtt atttttgcta gctcaaatca
    10921 tgccaccggt atgtatgaat ttgatagccc gtatagcgaa atcgtgaaag gtagttatag
    10981 tggtcttaca gctgattcca tcccattaat aaccacagat atgccggtta ggcctgatgg
    11041 gccttatggg attgctaaag cctttggaga agccgcgggc agattttatt cagaccagta
    11101 tggaatcagc tctatatcgg tacgaattgg aactcttaat tctgaaggta gacctacaaa
    11161 ccagaggcaa tttgctaccc tctttagcca tagtgatatg agcaatttat ttggaaagtg
    11221 catagaagcc ccttcaagct tgaaatatgg gatctattat ggggtgtcga ataataaatg
    11281 gagattttgg gatataagta actcggagag agatttaggg tacgctccta ttgatgatgc
    11341 tgagatatgg cgtaatggtt gattgactga gaattagtgt ggttaatata atctgccgta
    11401 ttagcttcat gcgttttatg aaggatgtgt agatttaatg gaacaagttt taacccctcc
    11461 accaataata gaacagttca agaaaaatgc tgtaacccac gttatatggt tgccagatag
    11521 tgaaacgaat ttcatgtatg agtcaatgac gagggaagaa aaattgaaaa tcgtgcctgt
    11581 gtgccgggaa ggggaatcga tggctatagc agctgggctg tgggtaggtg gtaaaaaacc
    11641 cgttgtctta atccaaaata cgggtatgtt tgaagcaggg gattctattc gtggcttggg
    11701 gattgatata gagtttccat tggtgatgat gattggctat cgaggttgga caaggcatgg
    11761 aataaccaaa gattcagctg ctcattttac agaaccaata ttgaatgcct acggcattaa
    11821 ctattattta atagagtcga atgatgatgt tgataggata agttcagctt ttgaggaagc
    11881 agaacgaact agaaggcctg tggcctgttt actgggggcg gaatacagct aatattatct
    11941 tacttaaagt cgttttccat ttcgatcact ttatttaatc ttcgtgcatg tctttcttca
    12001 tttgtatata cagcagaaat aaaggctgaa accagttccc gtactacttc ggcgccgact
    12061 atccgtgatc ctagacagag aacgttcatg tcgtcgtgtt caactccttg atgagccgaa
    12121 taaatatcat ggcatactgc tgcccttacc cctttgactt tgtttgcagc cactgaagct
    12181 cctaccccac ttccgcaaac cattatgcct ctatcagttt ttcctgaaga tacactctcc
    12241 gccagtggtt ttgcgtaatc cggataatca tctaaaggat ccaaaaaatg aggcccaata
    12301 tctatgacat catgcccgct ttttttgaga attgctaata tttcgttttt taattcaaac
    12361 ccattatggt cggcagcgac tgaaattctc ataggtgtta tctccggatg cagtagtatc
    12421 taccattatt agataaagag tttaggattt gacctgccgt acagtcaacg ccctttgaga
    12481 gaaattagaa tgctagaatg aggttcactt tgtgggaggt gcaaaatgtc ggtaagtcga
    12541 ttcgaaataa ccagtaaagt tttgttagaa gatgggaaag aatatggaga tatagggacc
    12601 tatgaccata tgcaaggtac agtctatttt gaaatagacc cattgttgga gtcaaacgaa
    12661 agaatcgtag atattcaatt agctccccgt aacccagatg ggaaagttga attttcagct
    12721 gatttcgttt tgttgacccc ttctgatcca gacagaggta atgggacgat gtttcttgat
    12781 gttgtaaata ggggaaacaa gaccgtactt tatggtttta atagttccaa tagacctaca
    12841 gaccctacct ctccaattga atcgggtaac ggatttttga tgagggaagg atatgcggtg
    12901 atgttctgtg gatggcaagc agatatacca gatatacctg gattgatcgg gctttcagta
    12961 ccggaagcat atgtcgacgg agaacaatta agtggcaagg tcatgaatca atatcaggcg
    13021 aatgttgcaa cttcagtttt tcctttggcc gaccgatatc atttgaaaaa cccggctgcc
    13081 gatgaaactg accctaaggc agaattaatg gtgcaagatc aaccaaacgg gatagcagaa
    13141 ctgatcgaaa gggataaatg ggctcttgtc cgggtggaag attctgaaat tgaaccggac
    13201 gtgtcgcatg ttcatcttca gggaggattc gaattaggac ggatatataa gctggtttat
    13261 acggcaaagg gtagtcgaat tgttgggtta gggtttgccg cggttaggga tatttgctcc
    13321 tttataaaat atgcctcgga agatgaaggt aatccactta gtggttacgt agatcatgcg
    13381 atatcttacg gagtgtcgca gaccggtagg tttcttaggc aatacatata cacgggtatg
    13441 aatgtggatg agtcttctcg tcaatcgatg gatggcataa ttgctcacgt cggcgggggt
    13501 atgagaggag agtttaatct ccgcttcgga caaccttcca aagatgtttg ctacataatt
    13561 ccagaattat tcccattcac tgatactgaa caaaaagatg cggtaacagg tagggaggat
    13621 ggactgttag acaggatggt tagtcaagga aatgtcccga aaataatgtt cacaaatagt
    13681 tctgccgagt actggcgtgg agatgctgct ttaatccaca ctaatataga aaataatagt
    13741 gatgccccag aacaccctaa tgttaggcgt tatcattttt caggaaccca acatggctct
    13801 ggaaaatacc ctttggagac ggtcagagaa tcagattctg taaggggtaa tttccctttt
    13861 aatggtgtca actacactcc gattttgaga ggttgtttaa ctaatatgaa taattgggta
    13921 aaaggcgaaa atgacccgcc gcctagtagt cacccaaaac atgaagatgg ctccgcgatc
    13981 gaaactaaag atgtaatagg aaaattttcg aagattccag gtataagact tcctgatagg
    14041 gtcttaaatc cgatgagatt agattatggc gatgagcagc atttggggag aacagtaaag
    14101 ttgccgccag aacaaggtga aacctatcct gcctttgtat cagatgtaga tgaaacttta
    14161 aatgagatag ctggtatacg tcttccagat ataagcgttc ccgtagctac taataccggg
    14221 tggaacacga gacattccct tattggaaac gaaggacttc ttattggaat taccgggggc
    14281 ttggccgggt ggacggtacc acttccttcc actgaaggtg aaaaggaaaa agatcatgac
    14341 cccagaccat caatcgaaag cctttattcc aataagcaaa attacatgtc ccagatacaa
    14401 gaagccgctc agaaattaat tgaggaagga tatattttga gtgaagattt tcaaggtgtg
    14461 gtggatatat gcgagcagaa gtacgacgat attaccggag ccgagtaata tggataaaat
    14521 atacggacac ttgcaatgca aatagtgcag acaagcgata attggctttc aaaagaagct
    14581 gtttttaaac acttgaatga gttggctgag aatacttaca cggaccgtac tgtctatctt
    14641 gcagctaatt tcaaagagag taaatccgtc actcccaaaa tcgcaggccc cgttatagag
    14701 gctgttataa atgaggttgg ttctaatgac accgggatgg ttatattcag acgtgatgaa
    14761 gaattaactg ttattgaacc acctttacca tttacgatgg atgcaatcat gcaagatcaa
    14821 gataccttgc ttttagaaga catatttgat gtacccaaaa cagttgcagt ggtcctggtt
    14881 agactaggta gatatgctgt tgccctaatg gaaggtcaga aattggtgga cacaaagact
    14941 gaaggtcgtc aaatgaaaaa tcgtcataag gcaggtggat cttcacagag aagatttgag
    15001 agaagcagag aaagattgat tcgagagtta tacgacaagg tctgcgaggt gtctcaacgc
    15061 atatttgaac caaggataaa cgatattgat tatctgtttc tgggtggtga gaaacacact
    15121 gtaaatggat ttaagaaaag gtgcggattt attggaaaat ttgatgggaa ggttatgtct
    15181 aggttgataa atattgatga accaaacagc gaggctctaa agtcggtagg ggatgaaatt
    15241 tataagagta gggtgagagt ctttaagaca gttagatgat tttctactta ttgggaagta
    15301 tatccgccgt cgatgacaag ggaagagcca gtgatgaagg atgcttcgtc agaagcaaga
    15361 aatagagctg cgttagctat ctcttccggt cgtcctaaac gccccatagg gtgcaaattc
    15421 tttaattgtt gataaatggg ctcattgtta gttaaagatt cagttaggtt agtctcgaca
    15481 tatcctgggc atagacagtt gacccgtaca tttttggatg ccatatcatt tgccaaagtc
    15541 tttgtgaatt ggatcacacc accttttgaa ggagggtatg cattaaaccc acctccgatg
    15601 ccgactggat atcctaccaa gccgtttatg gaggctaagt taattatgga tccacttccg
    15661 ttcagttcca tctctggtac tgcataccta gagaccagat aagtaccttt taaatttact
    15721 tctatcaccc tgtcccatac tttctcgggg tctgcatctt cctcaaaagc gtttcggctg
    15781 cttattccag cactgttgac taagatatcg agttttccat acgtttgtat agttcgattc
    15841 accatttcgg cagcatcacc tgatttggta acatcacctg aaatttttaa aatgctgccg
    15901 gtatctccca cgtagctatc cggaactgat atatctgccg cgacaacgcg ggctccctct
    15961 tccacgaatc tctcaacgca tgcttctcca attccagatt tggcacctgt tatgagggct
    16021 acttttccag ataaccttcc tacatttttt tttgcgcaca tatcgaactt cctaatctag
    16081 tattggacaa gattagcatg tatttaatct ttcggtagtt tgtgacagga atataaggct
    16141 gatagaatct ttttgctaga actacggtta ggaggagaag cactatgcct gaatttaaga
    16201 atcatgtcct ggtttgtgat gttgaaagag atgatgggat tcattgtgga aataagggtg
    16261 gcgcggaggt aaaacgaaaa gttgtagaaa tgttgtcgaa gtacggactc agaaaaagcg
    16321 tatatgtttc cagtacgggt tgcacgagtc aacacaggaa atgtgaaact gatcaatgca
    16381 gcatgattgt ctatgggcca ggaaatgaag gcacgtggta cattgcaaac cctgatgacg
    16441 tagaaacaat cgttaaagaa catctcgtcg acggaaatac agttgatgcc ctggtgaatg
    16501 acggaatgaa aataaatttg gatgcctaat tactagttta agaagctgat tctattatct
    16561 tgaaagtgct tctactgtaa aatacggctt tgccttttcc tttgattttg actccgttat
    16621 ctcccagaaa aacgccagta gcggagtcaa ttccaataat ctccattcct gactgtaact
    16681 cggattgatt ttcagacgaa actgtttcag ggacactatt ttcatggtga ggtaggacag
    16741 cagtgtttga tataaggccg atggctggca tccaacttcg gtatctcatt ttgctaccta
    16801 ataccattgc tcctgcactc gaaccggcta gagttgctcc tttcgatacg gcttctgtaa
    16861 tagaggccat gaatggtgtt tggtataaag tatttaacag gtgttttggg tctcctcctg
    16921 ttaaatatat gagatcgact ccatttaggt cattgcagta gatttcatct tctgcgtggg
    16981 ctttgtttaa aatcatgatt gggttgacat ttgctcccag ggcatcaaaa tgccgaaccc
    17041 cattctctgc agctttttca ggccgttggt ttgccgcggc tgtaggaata attgcgacac
    17101 gggggtgctt tttatttaag gattccacta aataagaatc catagcgagg cattcttcct
    17161 gaaattcccc acctccaacc aagagaatat tattcataag tttcttggtt tagactcgtt
    17221 aatctgatat ttctgcaatc atttctattt cgattggcat cccaccaggt agagttgcta
    17281 ctcccaccgc tgacctggca tgacgtcctt tatcaccaaa aatttctact agtaaatcgg
    17341 aacaaccatt tgtcacagct ggaggctccg tgaagtctgg tactgaatta accatcgaga
    17401 gtagcttgac aatctggtta actctatcta gttctcctag gtatcctttt aatctggcta
    17461 ccaagtttat tgctgtcagt ctagcgacat tatagccttc ctccacacca agagctccgc
    17521 cgagttttcc tgtataggca cttccgtcag gtttaggagt gggtcctacc ccggagaggt
    17581 atattaaatt acctgttctg accaccggta cataatttgc cataggagct ggcgaatctg
    17641 gtaattcgat tcccaattcc tgaattctgt cttctatcgt tcccattagg ttctcctagt
    17701 tggacttttc ttatcgttga tactattgtt cactactatg tagggcactt cggcacagag
    17761 cataatatca gaggggatcc aaatgagcct aacggcagat catgttagat tgaaggcggt
    17821 caatcatgtg acttacaacg tggttgataa agagagagca acaaaatttt gggtagacgt
    17881 acttggagta caacaaatac ctaaacaggt tgatgctgaa cacataattt ggctccaatt
    17941 accgagcgga gcgatggtac acattgttga aacccctgat ggcccctcaa ctccatcaca
    18001 tcacggtgct tttgaagtcg atgacataga tgcagcggct aatgtagtgc aacagaaagg
    18061 aatcgagacc actgacatta caaccagaaa tgatgggcag agagtttttt tcttgaatga
    18121 tcctgaaggt aatcgaatag aaatatgtac taaatcggga ttcggtgttc tagtctaatt
    18181 cttatacgat cgtaagtagt ctcgcgggcg tatttttggt tattgcctct atttcagatt
    18241 cgtggaaatt cctgtttcgc attcttggaa ttatgtgttc aggtatgtaa ccgtaaccat
    18301 ggccgccata tttcacaagt cggtgtttag tacaaatgtc atgagatatg acaattcggt
    18361 cacccaaacc attgtctatc atttgtctaa tgaaattgag gcgctgcgca tcactgggca
    18421 tatcaaagtc agaaggctgg taaaaagaca cttcgtttcc gaagagatcc cattccagaa
    18481 cacacccagt atcggctatt tttcctagca tttctattgt atctactgtc ctgtctaaat
    18541 gtcccataat cactctggaa atgtcggctc cggagtcaat gagtatgttg aggatttcaa
    18601 ttggagcgtt ttcgtccctg cccggatgta tcaggatgga ggctccggtc tctgattgag
    18661 ctcgagcaga tgctttcaga acttttaact catttgccat taagggccat gtacagccaa
    18721 tttcgccaat aattccggcc ttaattcctg tgctgcctac ccctatttga atatcctgga
    18781 ctatttcttg ataaatatca ttttcatcca tttcattaat tctgttaggt tgagctgcgt
    18841 cgacataata ccccgctccc atcaccacat ggagaccggc acctcgagat attcttgcaa
    18901 gcgctagtgg atctcttcct attccgacgg tggttgcgtc aacgattgcc ccacctccat
    18961 gcctttgata tagcgtggct tcttctatgg ctgtgtcctc atcaagtacc aagagattgt
    19021 cgagattact gtaggaatac tgtcttatcc accccagatt ctccatggaa accggtatat
    19081 gtgccaatct ttgcgttgtt acatctgggg aagggttgaa catgacagag aaatctataa
    19141 gtagatgttc atgagtcatt gttaaaccaa ggtccgctgg atctattaag cccagtactg
    19201 attgaatttt tcctttttgg gttgggtcac tcataatcct aacctataaa tgcttactga
    19261 gagttactat gtttgttggt catttaaaaa gcacgcattt atttcatgta ctggtccgaa
    19321 atctatctcg tggtcagagc taataaatgt ttctaccatg ccgactactt ttacattcca
    19381 accaacgaga ggtactaggc attgcgcttc actctagcgt ccagcggcat tgggtagttg
    19441 ttaaagtgtt gcaaccgctt atgaagcctc attatacctg cgtttagata atcattatga
    19501 tttaggcgga gagcggaaaa gcaaaagcaa ctttcggttc aatccatcaa tccatttttc
    19561 taaaagtctt gttgctcata gcaaaaccga tggtgaacaa agcaagtaaa ataccgctta
    19621 ttgtcaaaga atcggcagtc cctatttttt cagctaaagt accaaatgga acagcactta
    19681 aaggcatcat accgaaggtc atcatggtta tgctcatagc acggcctctc atctcagggt
    19741 cagctttaag ctgcaccata gtcatgttta gggacatgta cattgagcta gttaaaccga
    19801 taaatatgag aaacgggatt gccatagaga atgaggtgac ctgagcaaat atcgccaaag
    19861 ccacacccca ggctagggat gcacatagca taaccactcc tcttcttgga atattaccca
    19921 aagcagaaag tagaagggtt ccgattgtcg ctccaatacc cattagcatc agtagaattc
    19981 ccaacccatc agattcgata gccaatgctt ctcttgccca tgcaggaacc aaagccatat
    20041 aggaaaaacc aaaaagtact ggtatgaatg acataatgaa tagccctctc agagccgggt
    20101 tggcaaaggc gtaacgtatt ccatctgcca catccccgcc gacatttttg ccagttcttg
    20161 ctgatctgtt aggtgcatag tggaccattc caatagtgac agcagcgaaa acataaataa
    20221 tagaaataac aaaaaatacc ccagcggtat ctaacaataa aattaagaat cctgctaacg
    20281 cagggccagc gactctggtt aagttcatac tggagttact aagagatata gcattcatta
    20341 attttgaatc aggaactatg tcagaaataa ttgcctggcg gcttggcatg tttatggaca
    20401 ttaatgaacc attgaaaatt ccaatcacca taatgtgcca aaaagttaca attccaatca
    20461 aatcaagggt tccgagaatt aatgtaaaga ctccactgcc aatttggcta aaaacaatta
    20521 atttttttct ggaaaaccga tctgctaaag ctccagcaaa aggggccaca accgtcatgg
    20581 gtaaggcaaa ggtcaaagta acaagtgtga gtgccaaagg ggaatcgtct gtcaccctca
    20641 atataagcca acttcttgta atcatttgca tattcattgc catgaaagat acaaatgagc
    20701 ctatccatag ccatctgaag tccctatacg agagggcatc tagtatgttt gagaatcttc
    20761 ttttttttga tggcgtttgg tctgaattta tttcacaagg aatattcggt tttttaacag
    20821 actgggccat tgtgagtcac taagaaaggg ttacaatatc gaggttggaa tatttcagaa
    20881 ggtaacattg cggcatattc acgtcaatcg tttcatgtaa cctaattctg taataatctt
    20941 gtttcgtttg aacttattgt ttggagcgtt tttattatgg cgtcagaatc ttcacagtct
    21001 ttatggtcga gagattttgt tataaatctt ctggtggctc atctacttct cgctggtttt
    21061 ttctctcttt ttatggtggt tccagggttt atagacaaac ttggaggaaa ggaatgggag
    21121 ataggtgtca ttatcgcaac ttttggattg gccggggtaa ttttccgacc tatggcaggt
    21181 aaatggattt taaaatctgg gcccaaaaga gtaatcatgt tcgggatatt aatatttgtg
    21241 ggaaccactg ttttatacat tcccttgcct agtgagtggt ggatagtacc tgttcgtatg
    21301 gcccagggtg taggtcttgc gattgctccg gtggccacga gtaccgtggt tgccaatctg
    21361 gctcctgaat ccaggcgtgg ggagggtatg tcatacatgg gtaactcgat atctatagct
    21421 cagttatatg ccccaccgat cgggtttttc atagctgata gtttcagttt ttctttggct
    21481 tttatagtcg cctctgccgt ggcattagtg tcttttttcg tttgtctaac gatgtcagat
    21541 tctcgaacta ggatgcctat cacaccacag aatgaaaatg aaatgtctga tgcgcccttt
    21601 attagcagag cagcgatatt tcccactcta atctttttga cgtatgcgtt taccatggct
    21661 cctgtaagca ccttccttcc aatgctttcg gatgttaggg aattaggtgt aaatcctgga
    21721 ttttatttca ctatactgtc aggcatgacg atgctcacga ccctggtaag tggtcgtatt
    21781 gcagataaat ttggaagatc cgctgtaatt attcccggcc taacattagt ctctgtagcg
    21841 atgtttatgc tctccggatc ttttacttcg ggcatgtttt ttggtgcggc attttttcac
    21901 ggggttggat atggactgat ttatcctggt attaattctt tggtgataga ccgagttcca
    21961 gcgaatgaaa gaggctctgc tatcggtacc ctccagcaag cctgggatat tggcgcttca
    22021 gggggtaatt ttatagtcgg gccgataatt ggagccctag gggtcggttc cgcgtttgtg
    22081 gtagtgggag ccggggttgc cacgggagtg gttggattca ttactggaaa tcggaaatcc
    22141 aaaaactttt cgtagacctg ttaaatatca ctgagtacaa tgcacaaacg aattttataa
    22201 ttcgatgata gatcgttacg aagcttcagg agagacctaa tgaatggaga gcagttacgg
    22261 aaaaagacga gtagtggcgg tattgcttat ggaacgatgc ttagtatggg acgtaaccct
    22321 aggtggtcac aagcaatctc ttcatttggt ttagattatg taattattga tacagaacac
    22381 gctccccgta gtagggctga agtagcagat ttcttggcag ctttcacata cagcggagtg
    22441 gttcctattg tacggattcc aatccctgcg tctcactatg tgactatgta tattgatgca
    22501 ggtgcccaag gggtactcgc cccatattgt gag
//
LOCUS       DASCNS010000043         1574 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000385867, whole genome shotgun sequence.
ACCESSION   DASCNS010000043 DASCNS010000000
VERSION     DASCNS010000043.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000385867"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="QF838_01775"
     CDS             <1..528
                     /locus_tag="QF838_01775"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HJO59455.1"
                     /translation="SKFGLDNLVEELTNTSPDIAQTIDLDNSRRVMRALEKIHNDDRH
                     TLAAPKPDIQNTLNISVFGIQIPRTLLYPRVDARAEGMIRNGWISETHKLLDYGYGVD
                     LSSMSGIGYREISKYLHGDSTWQFCISKIKSRTHRLIRTQNNWFRRIDSSITWFDSHE
                     LNTFEISNKISCLMN"
     gene            612..1451
                     /gene="dapF"
                     /locus_tag="QF838_01780"
     CDS             612..1451
                     /gene="dapF"
                     /locus_tag="QF838_01780"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HJO59456.1"
                     /translation="MKFTKMHGAGNDYVYVNGFEYDLDWKHISQTVSDRHKGIGADGL
                     IVACPSSNNSDLKMRMFNADGSEGEMCGNGIRCLVGFARDESLINGDLQVITVETMAG
                     NLDVFPILEDSRMIGAKVSMGSPNLVPKNVPVQIGSDEPTLDYSLDVDTHELSLGFVS
                     MGNPHAIAFIEEDVDNFPLELIGPKIENHDIFPNKVNFEIVNALSPSELKVRVWERGS
                     GITMACGSGACAVAVAARLKGITLDDVNLKLPGGDLKVRWDGVGEVELEGPIETVYKG
                     VLQ"
BASE COUNT          519 a          293 c          357 g          405 t
ORIGIN      
        1 tcaaaatttg gtttggataa cctagtagaa gaattaacca atacttcccc agacatagct
       61 cagaccatcg atttagacaa ttcaagaaga gtcatgaggg cactagaaaa aatccacaat
      121 gatgatagac acacacttgc agcaccgaaa cccgacattc aaaatacact aaacatatct
      181 gtgttcggta ttcaaatacc caggaccctg ctatacccca gagttgatgc aagagctgag
      241 ggtatgatta gaaatggatg gatttcggaa acccacaaat tgctagacta tgggtatggt
      301 gtggaccttt cgtccatgag tggtattgga tacagggaaa tatcaaaata cctgcatgga
      361 gattcaacct ggcaattttg tatctcaaaa ataaagtcac gtacccatcg gcttatacgc
      421 acccagaata attggttcag aaggattgat agctcaataa cctggtttga ttcccatgag
      481 ttaaacactt ttgaaatatc caataaaata tcgtgcttaa tgaattgaac aacttcccca
      541 agcctatatg atccattcga tgttagaatt cctaccactt aatgcaatac ccagttagat
      601 agaagttaga tatgaaattc accaaaatgc atggagccgg aaatgactac gtttatgtga
      661 acgggtttga gtatgatctt gactggaaac acatatctca aaccgtgagc gacagacaca
      721 aaggcatagg agcagatggt ctcattgttg catgcccatc gtctaacaac tcagacctaa
      781 aaatgcgtat gttcaacgca gatggttcag aaggagagat gtgtgggaac ggaatacgct
      841 gcctggttgg ttttgctagg gatgaaagtt taataaacgg tgacttacaa gtaatcacag
      901 tggagacaat ggcaggcaat ttggacgttt tcccaatttt ggaagatagt cgaatgatag
      961 gggcgaaagt cagcatggga tctcctaatt tggtcccgaa aaatgtaccc gtgcaaattg
     1021 gctccgatga accaactctc gactattcat tggatgtgga cacacacgaa ttatcccttg
     1081 ggtttgtatc aatgggaaac cctcatgcta ttgcctttat tgaagaagat gtagataact
     1141 tccccttaga attgataggc cctaaaattg aaaatcacga tattttccca aacaaagtga
     1201 attttgagat tgtaaatgcg ctaagcccat cagaactcaa agtgagagtt tgggaaagag
     1261 ggtctgggat cacgatggcc tgtggtagtg gagcctgtgc agttgctgta gcagccaggt
     1321 tgaaaggaat tacattggat gatgtcaact tgaaactacc cggtggagat ttaaaagttc
     1381 gttgggatgg ggtaggggaa gtagaattgg agggaccgat tgaaacagta tacaaaggtg
     1441 ttctccaata ataattatct agggatcggc tggaaacagg agttaaataa aaatggaatt
     1501 cgcaaatcgg ttagacacgg taccaccgta tctttttgta gaaataagca gaaaaatcgc
     1561 tgccaaaaaa gcac
//
LOCUS       DASCNS010000044         1234 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000392393, whole genome shotgun sequence.
ACCESSION   DASCNS010000044 DASCNS010000000
VERSION     DASCNS010000044.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000392393"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="QF838_01785"
     CDS             complement(<1..836)
                     /locus_tag="QF838_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HJO59457.1"
                     /translation="MDGNHLVLASMDGHLMVLDKDTGKHIWKPDLMRVHEEDENKRAV
                     YGLPAVSNGIVFVGVYDGKIQAIEVDTGRIVETEVVSDDFEIVGGPLIHEDNLYIGSG
                     DGILRSYELDFSNQQVTFNDDWSYQLGGEIWSTPVIHDDILIITSLDHHVYALNKDTG
                     ELVWKVKTGGAIAATPVIQEDTVFVGSFDGVFYAFDLNTGDEEWVFEKASNWYWGGAV
                     ISDKTIYIPSLDGKLYALDIRSGKKEWELETNGPIVGSPAIVSDMIAVGSTDGEIRIA
                     ET"
     gene            complement(985..1218)
                     /gene="yidD"
                     /locus_tag="QF838_01790"
     CDS             complement(985..1218)
                     /gene="yidD"
                     /locus_tag="QF838_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013932.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HJO59458.1"
                     /translation="MKLRKDNFVKSTVLGLIKSYKKIISPFLPIACRHIPTCSDYAYT
                     AIHQYGLAVGSGITFKRLLRCRPFGTRGYDPVP"
BASE COUNT          359 a          290 c          209 g          376 t
ORIGIN      
        1 ctcgtttcgg ctatccgaat ctccccatca gtggaaccca cggctatcat gtctgaaaca
       61 atagcaggag acccaacgat tgggccattc gtttctagtt cccattcctt tttaccagac
      121 ctgatatcca aagcatacag ttttccatct aatgacggaa tgtagatagt cttatcacta
      181 attactgcac caccccagta ccagttagag gctttctcaa aaacccactc ttcatcgcct
      241 gtgttcaaat caaatgcgta aaatacaccg tcaaaagacc ccacaaaaac agtatcctct
      301 tgaattacgg gagttgctgc aatggcacca ccagttttta ctttccagac aagttcacct
      361 gtatccttgt tcagagcata tacatgatga tcaagtgaag taattattag gatgtcatca
      421 tgaattactg gggtagacca tatttctcca cctaactgat atgaccagtc atcattaaaa
      481 gtgacttgtt gattggaaaa atccaattca taagatctca atatgccatc gcctgaccca
      541 atgtaaagat tatcctcatg aattaatgga ccgccaacta tttcaaaatc atcgctaacc
      601 acttcagttt caacaattct tcccgtgtcg acttctatag cctgaatctt tccgtcgtac
      661 acccctacaa aaacgattcc attggatact gctggcaaac catatacagc tctcttgttc
      721 tcgtcttctt catggactct cattaaatct ggtttccaga tgtgtttccc tgtatctttg
      781 tctagaacca ttaaatggcc atccatgcta gcaagaacga ggtggttacc gtccaccaga
      841 acacctgacc aaccctgggc gctattaatt cttgagcatc ccaaaatagt gatcaggagt
      901 aaaagtagac acactgatat ttttttgttt ctagaaatca tatttcaatt aaatttggtc
      961 ttcctcagtt ggtatgattt tgagtcacgg aacagggtcg taccctctag ttccaaatgg
     1021 ccgacacctc agtagccgtt taaaggtgat tccactacca acagctagac cgtattgatg
     1081 gatcgctgta taagcataat cggagcatgt cggtatatgt cgacatgcta ttggtaaaaa
     1141 gggtgagatt atttttttat aagattttat taaacctaat actgtggatt taacaaaatt
     1201 atctttgcgg agtttcaatc tatttcacgt ttac
//
LOCUS       DASCNS010000045         5054 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000393289, whole genome shotgun sequence.
ACCESSION   DASCNS010000045 DASCNS010000000
VERSION     DASCNS010000045.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000393289"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(62..1864)
                     /locus_tag="QF838_01795"
     CDS             complement(62..1864)
                     /locus_tag="QF838_01795"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HJO59459.1"
                     /translation="MSTTNKTLLSCIGLIIFVFTSSHSLIQISADQNGQSDHRIIILG
                     IDSNSVVSKKTHMEMVDSIIGTMSTIRQGDDFYLMTMDSPDNYIGPYRAGETDFEEFP
                     GKVQDIISNSEDNSSLGVAVDQTLGESYGLLDIEGAPPGSGIYLISTNEYPDHKDYVG
                     HRLDPLSDLFYERGWHISTFALSGIPTSNLEVSTNLATATEGNIFQIADIRGLKLFTD
                     YLLTTDNRGKLISSGEKKLTFSNLLISDIEILPGTEYVMALFFRESNAGSFRLKNPDG
                     LEASTGDRSSSSVLETPNAVIWIINEPAPGNWNVDVTGISGLVSSWYLASNKYSLNFQ
                     MPEVVPIDEAATISAFITEAQSISIMDKDAVISADVATPDNTSITYTLKDEGVDGDLK
                     SGDGYYSAKIPQPLAEGTYSVSVHLSWPNVKYNLTRDFIFQAQVFPTIEIEPLYTTNL
                     FIGEEYTVATLYTRIKDSPHAVLPADINTQILNDLQFDGTLKINPRSPDSEGKAWMYD
                     VIFSPDKPGQTSAIFNVDLNYANRNYSRATENLILESRVIPHSAAQPYSNVPYQPESN
                     APLPSTNISTIMDIRETIIIFIIIICNISRRNFC"
     gene            2057..2413
                     /locus_tag="QF838_01800"
     CDS             2057..2413
                     /locus_tag="QF838_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59460.1"
                     /translation="MDRGMCKPFFFNVHLMCVLLVIGNLSCGGDRIGISDVSHVEVSG
                     LIIDFEAGSLTDIDHLTIETEVGQIYQLNVGRNFGEFTPSHIRQHMVHGELVEISYFR
                     RDGEYWLRNIRDFTFR"
     gene            complement(2400..2945)
                     /locus_tag="QF838_01805"
     CDS             complement(2400..2945)
                     /locus_tag="QF838_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024515.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HJO59461.1"
                     /translation="MSKLILLNLLAAILVTLWGQTTQLEAHGNMKNIHFGTAGEYDLK
                     VSIIPHEPVSGSVHFHIEPTLSSTTEPLEKAIVTVVIRQGENAFQSRAVNSPSSPTIY
                     DANLTFETPGGWEVEIKISTKPDIEDKISFQLYVLDSGSPTSNAAGIFFLFVFSVLIT
                     SSIFLYIRYGRKPKNKIVNEK"
     gene            complement(2970..3045)
                     /locus_tag="QF838_01810"
     tRNA            complement(2970..3045)
                     /locus_tag="QF838_01810"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3010..3012),aa:Ile,seq:cat)
     gene            3180..3929
                     /locus_tag="QF838_01815"
     CDS             3180..3929
                     /locus_tag="QF838_01815"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO59462.1"
                     /translation="MGRLSGKVAIISGGARGQGAEEARLFASEGAKVIIGDILDSEGI
                     QVESEIVERGGQAKFVRLDVSIEDDWSKAVQLAISEYGKLDILVNNAGILLMKGVEDT
                     SSEEWDYLHNINSKGVFLGSKAVISAMRESGGGSIVNISSIAGLIGSRFSAYGASKGL
                     VRTLTKSIAVNHGHENIRCNSVHPGIIDTDMVSEIIGSKEGRDLQLNRTPLNVIANSR
                     DVAFGVLYLASDESRYVTGSELVIDGGITAQ"
     gene            3907..4833
                     /locus_tag="QF838_01820"
     CDS             3907..4833
                     /locus_tag="QF838_01820"
                     /inference="COORDINATES: protein motif:HMM:NF020123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HJO59463.1"
                     /translation="MVESLPNKFFSLRNPYKVDIFPIWVVLFALLIGCSTGADSASNA
                     IAEPEISKPQVVANNKSADEARDLSASKTVIVKSNALPSKEPEPATHLPTAVPKNAST
                     SLTSLNDKKLATGFSNVVDEIVEDKVTKPYEKEGFYREAKVQIRGKYLPKLVQGYEDS
                     ALGWAAPEVKAVGLDGEEIYIGGEGPTTLIMVLAHWCPHCRNEVRELAPFLNETDSLD
                     RVRMVTLLTSINDERPNFPPHTWLDLEKWPVPSMVDTPSSQIATAYGISSFPFFIVIN
                     DKGEIALRMPGRLGIETLSRLLDAIGDMGEEN"
BASE COUNT         1469 a          906 c         1148 g         1531 t
ORIGIN      
        1 ctggtattta gatgatgatg gtgatggttt aggtaatata acttttacct gggatggatg
       61 ttcagcagaa gttccttctg gatatgttgc aaataataat gatgaagatg ataattgttt
      121 ctctaatatc catgattgtg ctgatgtttg tgcttgggag aggggcattt gattcaggct
      181 gatagggcac attggaatat ggttgtgcag ctgaatgggg aatcaccctt gattccaaaa
      241 tcaaattttc tgttgcccgt gaataattcc tattggcata gtttaaatcc acattaaata
      301 tagctgaagt ttggccaggt ttatctggag agaaaatcac atcatacatc caagctttac
      361 cctcggaatc aggtgatctg ggattgattt tcaaagttcc atcaaattgc aaatcgttaa
      421 gtatttgggt attgatgtca gctggtaaaa cagcgtgagg agaatcttta atccttgtgt
      481 atagagtggc tactgtgtat tcctcgccaa tgaataaatt agtggtgtaa agtggctcga
      541 tctcgattgt tgggaatact tgggcctgaa agataaaatc tcgagtcaga ttgtatttaa
      601 cattgggcca gcttaaatgg actgacaccg aatatgttcc ctctgcaaga ggttggggaa
      661 ttttggctga gtaatacccg tcaccggatt ttagatcacc atcaaccccc tcatctttca
      721 gtgtataggt tatggatgtg ttatccgggg tggcaacatc agcactaata accgcatctt
      781 tgtccattat cgatatcgat tgagcctctg ttatgaaagc agaaatagta gctgcctcat
      841 ctatgggtac gacttcgggc atctgaaaat tcaggctata tttattggat gctaagtacc
      901 atgatgatac aaggccgctt attcctgtta catcgacgtt ccaatttccg ggggcaggtt
      961 cattgattat ccatatcaca gcattgggtg tttctaatac agaagaggaa gacctgtcac
     1021 ccgtggaagc ttcgagtcca tcaggatttt taagcctaaa tgaaccggca ttactttccc
     1081 tgaaaaataa agccattacg tattccgtac caggtaaaat ttctatatct gagataagta
     1141 ggttgctgaa agtcaatttc ttttctccag aagaaatcaa tttccctcgg ttatcagtgg
     1201 ttaataagta gtctgtaaat aatttcaatc ctcggatatc cgctatttgg aatatgttcc
     1261 cctcagtagc agttgcaaga ttggtagaca cttctaaatt ggatgttgga attcctgata
     1321 acgcgaaggt agatatatgc cagccccttt cataaaaaag atctgataaa gggtccaacc
     1381 tatgcccgac ataatcttta tggtctggat attcgttagt actgatgaga tatattccag
     1441 acccaggagg ggcaccttct atatctaaca aaccataaga ttcaccaagc gtctgatcta
     1501 ctgctacgcc caacgaggag ttatcttcgg aatttgaaat gatgtcctgc acttttcctg
     1561 ggaactcttc gaaatcggtt tccccagccc tataagggcc aatataattg tcgggtgagt
     1621 ccattgtcat tagatagaag tcatcccctt gtcttattgt cgacatggtt ccaattattg
     1681 aatccaccat ttccatatgg gtttttttag aaaccacaga attcgagtct atcccgagaa
     1741 tgattatccg atggtctgac tgaccatttt gatcagcgga tatttgtatt aagctgtggg
     1801 aggaggtgaa cacaaagata ataagaccga tacagctcag gagggtctta tttgttgtac
     1861 tcataaacca cttttaactt atacgctttt gagggccttt tttacaggca gcgatttttg
     1921 gatttgtata aaaattatgt tctaacttta tagtaattta tgttagattg aacccgttgc
     1981 aagttcaatc taatttagga actatttttg ccgaacctgt gtgaatgttt acaagtgatg
     2041 cgggtgtaat aaatatatgg atcgtgggat gtgtaaaccg tttttcttta atgtgcatct
     2101 aatgtgcgtt cttttggtga tagggaattt atcctgtggt ggagatcgca ttggtataag
     2161 tgatgtctca catgtggaag tgtctggttt gattattgat ttcgaggccg gctcactcac
     2221 ggatatagat cacctcacca tagagactga ggtagggcaa atctatcaac taaatgttgg
     2281 tagaaatttc ggagaattca caccatccca tatacgacaa catatggttc acggggaatt
     2341 ggtggaaata tcatattttc ggcgtgatgg tgagtactgg ttaagaaata tacgagattt
     2401 cacttttcgt tgacgatttt atttttgggc tttctcccat aacggatata taaaaatatg
     2461 gaactcgtta taagcactga aaatacaaat aggaagaata ttcctgcggc attacttgta
     2521 ggacttccag aatccaaaac atacagttgg aaactgattt tatcttcaat atctggctta
     2581 gtggagattt ttatttccac ttcccatcca ccaggggttt caaaagttaa atttgcgtcg
     2641 tatatagttg gtgaggaagg ggaatttaca gctcttgatt gaaaagcgtt ttctccttgc
     2701 cgaattacaa ccgttacaat ggctttttca agaggttcag ttgtagatga caaggttggt
     2761 tcaatgtgaa agtgaactga acctgatacc ggttcgtgag gaattatact gacttttaaa
     2821 tcatattcac cggcggtacc aaagtggata tttttcatat tgccatgggc ttctaactgg
     2881 gttgtttgtc cccataacgt gaccaaaatg gccgccaata aattaagtaa aattaatttg
     2941 gacaatcaaa actcgttcct tttttgtaat ggtgggcccg gcagggatcg aacctgcgac
     3001 caatcggtta tgagccgacc gctctaacca ctgagctacg ggcccaaatg tgagttgtgc
     3061 ctactaataa tctgtagcac gaacaaaaat ttgcgtcctt aattgtcctg taagcgaagg
     3121 tcgtagtcaa ggaaagcctc tgataatata ggttcaaatt aatccacacg gagaatgaca
     3181 tgggtcggtt atctgggaag gtggcgataa tatccggtgg tgctagaggg cagggtgcag
     3241 aagaggcacg cctttttgca tccgaaggag ctaaagtaat catcggagac atactggatt
     3301 cagaaggaat tcaggtagaa tctgaaatcg ttgagcgagg cgggcaggct aaatttgttc
     3361 gtcttgatgt atcgatagaa gatgactggt caaaagctgt tcagctggct atatctgagt
     3421 atggcaaact agatatattg gttaataatg ccggtatatt gctgatgaaa ggggttgaag
     3481 atactagtag tgaggagtgg gattacctcc ataatatcaa ttctaaagga gtttttttgg
     3541 gttctaaagc agtgatctca gctatgagag aatcgggtgg aggatctata gttaatatat
     3601 cttccatcgc gggtttgatt gggtctagat ttagtgccta tggtgctagt aaggggcttg
     3661 ttaggacatt aacgaaatct atagctgtga atcatggaca tgagaatata aggtgtaatt
     3721 ccgttcatcc tggaattatc gacacagata tggtttcaga aattattggt agtaaagaag
     3781 gtcgagatct tcagttgaat aggacacctc taaatgtcat tgctaattct agagacgtgg
     3841 cttttggggt tttgtatttg gctagtgatg aatctagata tgtcacaggc tcggaattgg
     3901 ttatagatgg tggaatcact gcccaataaa tttttctcgc ttagaaaccc ctataaggtt
     3961 gatatatttc caatatgggt agtcttattc gcgttgctga taggatgcag caccggggca
     4021 gactccgcct ctaatgctat tgccgaaccg gagatttcaa aaccccaggt cgtagctaat
     4081 aacaaatctg ctgatgaggc gagagatttg tcagcctcta aaaccgtcat tgtcaaatct
     4141 aatgcactac catctaaaga gccagaaccg gctactcacc ttccaacggc cgtccctaaa
     4201 aacgcgtcta catctctcac atcacttaac gataaaaaac tcgcaaccgg tttttcaaat
     4261 gttgttgatg aaatcgttga ggataaagta actaaaccct atgaaaaaga agggttttat
     4321 agggaagcca aagtacaaat cagaggaaaa tatctgccaa aattggttca aggctatgaa
     4381 gattcagcat tagggtgggc ggcccccgaa gtaaaagctg ttggcttgga tggggaagaa
     4441 atttatatag gtggtgaggg tccaacgaca ttaataatgg ttctcgcaca ttggtgccct
     4501 cattgccgaa atgaggtgag agaattagcg ccatttttga atgaaactga ttctctagac
     4561 agggttcgga tggttactct attaacttcc attaatgatg aaaggcccaa tttcccacct
     4621 cacacatggc tagatcttga aaaatggcct gttccgtcaa tggtagacac cccctctagc
     4681 caaattgcca ctgcctacgg tataagttct ttcccttttt tcattgtaat aaacgacaaa
     4741 ggtgaaatag cacttagaat gcccggaaga cttggaattg aaactctgag tagattacta
     4801 gatgcgatag gcgatatggg tgaagaaaat tgacggttat aatccaaatt gttcaaaagg
     4861 ttgaatggag ctaacaaaat ggcgatttct aacaaggttc gcgggttcat ggaaaaaggt
     4921 tcttggataa gaaaaatgtt tgaagaaggc atttctctta agcagcaata cggagaggaa
     4981 aatgtttttg atttgtctct aggtaatcct gtgatgaacc cgcctgcaca attttataag
     5041 gagttaaaaa agat
//
LOCUS       DASCNS010000046        26207 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000393400, whole genome shotgun sequence.
ACCESSION   DASCNS010000046 DASCNS010000000
VERSION     DASCNS010000046.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 26207)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26207)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26207
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000393400"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="QF838_01825"
     CDS             complement(<1..273)
                     /locus_tag="QF838_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59464.1"
                     /translation="MFDNPNNDLNVFAYWVSQGKLAAGEYPGNQWSFRPSTTIATIVN
                     SVSGMFKAKFRDKNTPSYKIGNLMECGIGCFIDLTEPGERPDLAPCE"
     gene            340..894
                     /gene="orn"
                     /locus_tag="QF838_01830"
     CDS             340..894
                     /gene="orn"
                     /locus_tag="QF838_01830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HJO59465.1"
                     /translation="MVSKLDKDNNLVWIDLEMTGLEDDHLIIEIASIVTDGNLKELAE
                     GPSLAINRTISEMQNINDWSKEQHQKSGLLKRVRESTVTVSEAENQTLAFLKEWVSVG
                     MSPICGNSIGTDRRFIRREMPQLDIFLHYRMVDVSTIKELARRWYPKTNLPEKEGKHL
                     ALEDIRESIEELRWYRKTFFNIEI"
     gene            915..1862
                     /locus_tag="QF838_01835"
     CDS             915..1862
                     /locus_tag="QF838_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO59466.1"
                     /translation="MIKRILGKTGLEVSLLSLGTGGARQLGQTIGHSIYDQKKLVEAA
                     LDTGINIFDTATHYGNSESILGKCLSDKPRDSFILCTKWPARDELNNTIDDPTKLIKS
                     VEKSLDRLQTDYIDIMLFHGIMPQEYDLIIETLYPTMERLKSEGKIRSIGFSSRFSED
                     PAQEGIKLGLSKHPELWDIVMLKYGILNQSAGENILPLARTHNIGVINMAAVRIKLPD
                     PILLEQLIAEWKYSGLIDKDSLPAHDPFGWLVEGNVKSVIEAGYKFAAEPTTVSTVLT
                     GTASIEHLMANVECLEQPYLPQHHTDRLKTTLSHIIQYA"
     gene            1915..3597
                     /locus_tag="QF838_01840"
     CDS             1915..3597
                     /locus_tag="QF838_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HJO59467.1"
                     /translation="MDENTNQDFIRDIVKEDLASGKHQFITTRFPPEPNGYLHIGHAA
                     SICLNFGIAKENESAKCNLRFDDTNPSKENTEFVEAIKKDIQWLGFDWDEREYFASDY
                     FQKLYGFAMTLINQGKAYVDSQTQDEIRANRGTLTEPGADSPYRDRNPQENTKLLEEM
                     RNGIHRPGSHVLRAKIDMASPNLNMRDPVMYRILDTPHHRTGNEWPIYPMYDFAHGLS
                     DSIEEVTHSLCTMEYEDHRPLYEWFIDELKVFKSRQIEFGKIFLSHTILSKRNLTKLV
                     ENKLVSGWNDPRMPTISGMRRLGYTADSIREFCRRVSITRRWNTAEIELLEHCLREDL
                     NKKSNRALAVLRPIKLVIENYPEDQVEYLEAANNPQDRDSGTRKIPFSKELFIEQDDF
                     MENPPKKFFRLTLGREVRLRYGYLITCTEIIKDPITDEVLELRCTYDPETRGGNAPDG
                     RKVKSTIHWVSANHSINAEVRLYERLCLDSEPDISDGENLETVLNPNSLEIIRSAKVE
                     PSLKESTPGNPMQFERLGYFCLDSEDSKNEGNLVFNRTVSLRDSWAKTQSSN"
     gene            complement(3608..4891)
                     /locus_tag="QF838_01845"
     CDS             complement(3608..4891)
                     /locus_tag="QF838_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59468.1"
                     /translation="MKLTESPTLFSPFKWRHAVFYGWWLVVITIFLNAATGSPVFGGV
                     GVWIDALEQHFGWSRAELSLAFSFGQLESGLAGPVVGILVDRYGPKKIVFSGVTIIGL
                     GFLIFSQTNNLWTFYCAYIVIMLGASAGGWLPMMSVINNWFDKKRTMAMGLGGIGFSV
                     GSFFLVPLLAWLVQPEGVGWRTTSLSLGIFFILIAFPISRLIRNSPEELGEIPDGRVF
                     EVKDSDRSVTTRIDNDFPMMTIIKMPVFWVLAICNGTSAMLIGTMTVHLILALKDQGI
                     SVQTGAWIWGATMLFSGAAQVLGGYLGDRVPKNLSLCVFGIIQALGVIYATFITSVYM
                     ASIFVFIYGLGFGARIPLGTAIRGEYFGRKAFGRVLGVSMLPMMTLMMIGPWVAGFMF
                     DHYGSYDRAFYILASVALVGSVGFLFCKNPNQSRS"
     gene            complement(5022..5876)
                     /locus_tag="QF838_01850"
     CDS             complement(5022..5876)
                     /locus_tag="QF838_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59469.1"
                     /translation="MFYDTHSYREPLEPMRDDRTHALVNILPRESKRLIAKGVLAMPE
                     VERVLKQGWFVISRGITPSYILEELTGQEFDTANCTAGIITEGRMASVLEEDRLGPWV
                     FKDGQLDETPAPQALSEFTAYDVSIKGANAVDSEGNIGVFAADKAGGTVGGIWPTLTA
                     RGAHWVAPVSLERLIPSVIEAAKHCGNHLWDYTMGQSAGFMPVVNAQVITEIQALELL
                     TGVTATHVGSGGVAGSEGAVMLALEGDHDTVLKAFELVESIKGEPQFDSPRLVPYVRE
                     PEATPPAR"
     gene            6023..6541
                     /locus_tag="QF838_01855"
     CDS             6023..6541
                     /locus_tag="QF838_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO59470.1"
                     /translation="MVEQQLQILRPETLVAEFKSIGVTHIITIPDSETNYLYELMEKE
                     DWLDVVPVSREGESMAVALGLNVAGKVPVVLIQNTGMMESGDSIRGMALDAGFPLLMV
                     VGYRGWTRKGHTPDSAAVYTEPFLNAFHLNYYLVETDDDGARITDAYNEAVKTKRPVV
                     VLIGDEYHGFNR"
     gene            6570..7154
                     /locus_tag="QF838_01860"
     CDS             6570..7154
                     /locus_tag="QF838_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO59471.1"
                     /translation="MIESKEVFEAFQEHRGNAIVSVQGTSGKHWADVTTNQNRDISLG
                     GAMGQSTAAIFGLALGLPNDKVVHFDTEGALLMNLGVLASIAGKKPANFVHFLLDNGC
                     YATTGGQPVPNSEDIDYSVIAAGSGYAATYNFDDLEELSTSLDEIMNQDGPVFVAIKV
                     PAEVENLPIGMRERRVSRNRGQTISDLRSELGIS"
     gene            7402..8271
                     /locus_tag="QF838_01865"
     CDS             7402..8271
                     /locus_tag="QF838_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJO59472.1"
                     /translation="MPEFKNILYEIDGPVLTITWNRPDALNAISQNLEREFHHALDIA
                     AENEAIRSIIVTGSGRAFSAGYDISGDRSKRPRLPTSTHLKRQWGSSMLSTGGPTHIM
                     HLMEHPKPIIAAVNGWCIGGGFWYSLACDITIASDQATFGQPEIRMTSNTTVLFALLC
                     GWKNAHRYALTGDHFDAQEALRIGAINEVVPHDQLLEKARALALRLAKVPSESIRFNK
                     AITTYGLDAMGLRSALNMNGALSTIVEAANDGPDVEHLDRAQAEGSFGAFLKARDEPF
                     LPEPFGPRSKPRD"
     gene            complement(8600..8971)
                     /locus_tag="QF838_01870"
     CDS             complement(8600..8971)
                     /locus_tag="QF838_01870"
                     /inference="COORDINATES: protein motif:HMM:NF039961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6027 family protein"
                     /protein_id="HJO59473.1"
                     /translation="MLFDDSVILEQFIWESNVSDKDNNFKNDVALYTQEDPLPTVRRL
                     SRNLDIPIGSVVRYILCKWAMSGSESLLDLGPDMVRKISDIFEDAESDGTDEARLEAY
                     NSVKAIMSWMKVPLDNPNYRS"
     gene            complement(8981..10420)
                     /locus_tag="QF838_01875"
     CDS             complement(8981..10420)
                     /locus_tag="QF838_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013799.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HJO59474.1"
                     /translation="MTKIILAVFDGLQPAQINSVDTPNLFRVSQNGAFFENHHPVFPS
                     VTRVNAASMVTGVNPGKHWLAGNSFVARDYDDSKVILALNVQLSEIRNAGLEVLGVPT
                     LQEIISEKSMEYVAIGVGTSGNAYVHNPLADVYGGATIHPEFTIPHRLHKELEGLFGG
                     WPEEQLPNTPRYKKAVDIFIEYVLGKINPEVALIWSSEPDKSQHASGVGSDAAQAALR
                     QADAEFGRIMEYISTSSQHQNSDLMILSDHGYSTITEVIDIETLLESSNLAGSDGWLL
                     AQNGGCVLFYLKNQEDVYLIPELVEWLSGQVWCGTLCASERLGEVKGTIPLSSIMNEG
                     KRSADIIMSFHWDSSDNVDGYAGHVFSTGGAKNLGQHGSISLHEMNNTLICSGPTFLE
                     GEKILSPTGNTDILPTILSILGEELPAHVEGRILEESFKGSDNEIISEMKIHEASLST
                     EFGTYSQEITISAVGQTQYIEEGRSWLKK"
     gene            10639..11499
                     /locus_tag="QF838_01880"
     CDS             10639..11499
                     /locus_tag="QF838_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HJO59475.1"
                     /translation="MTDSQKRHHFRQILSGNRCVHPGSVFDAISSRIAEDLEYQVGMF
                     AGSIASAVVLGDPDHILITLTEFAEQARRICRASDLPIMVDADHGYGNALNVKRTVEE
                     LENAGVAALTIEDTVLPKQFGKSEPELISLPEGIGKMKAALSGRKDQSLVIAGRTSAF
                     QVTGTEDAIERAKAYESIGVDAIFFAGLTTRSQLEAVKNSIAIPIMLGGTPKDINDDQ
                     YLSSMGVKIALQGHLPFMAAVKATRDTMKALIEGKQPSEVSTTALDSELIDKVTKDQQ
                     FKDWFKEFLS"
     gene            complement(11507..12547)
                     /locus_tag="QF838_01885"
     CDS             complement(11507..12547)
                     /locus_tag="QF838_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59476.1"
                     /translation="MRLALFTHMSWPEGVNQTSVFHDSVEQVKLAESMGYHGAWFAEH
                     HFTRYSMGSSVLLMLGHLAAVTEKIRLGTAVIVPNLHNPIRIAEDSALVDLLSRGRLD
                     VGFGRGTYGYEYGGFGVPAEDSQSRFRETVLAVRDLWCDKDVSVDSPFFSVRNVDLVP
                     SVVQSPHPPIYIAATRSEETLDFMISNQFLLCIAVVQDTDAALALVEKYRRLCHLKDQ
                     SDHMDEVPFFRYVHVADTERKALDNTKKHVDWIQDIMQWRRNLVGTTEVHLPIKKWRN
                     ERDELPPDNRYISGKRAFIGTPEAVAEKIISLKDHGISYFGCNFAMGGLPQEEVIKSM
                     KLFEKKVIPLIS"
     gene            complement(12553..13611)
                     /locus_tag="QF838_01890"
     CDS             complement(12553..13611)
                     /locus_tag="QF838_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59477.1"
                     /translation="MKIAVRSFSGVRSDLSWSDPSDWNQIADFAKEAESLGADSIWTS
                     EAWGTDAITPLAYLAASTTKIRLGTGIVQVGSRTPGNLVMTSLTLQSMSGGRFILGLG
                     TSGPQVIEGFHGVTFGNPVLRTKELLEIIKMGVSGEKLQYQGKYYQLPLDGGEGKSIR
                     TSAKTWHVPVYVASLGPSNLEMTGEKADGWIGGCFIPETGNVFLDKIKEGLFRSGRSI
                     EDIDILIPVSLDFTDDVEETAKRHARGYGFTFGAMGSVKNNFYKNAYARQGYGEIVDV
                     VQKLWVEGRREEARDMVPNELALRSNLIGTDKMIKERLLLYKNLGVSTLKVDLPGDDI
                     ASKSDALGRLMDLVSDLD"
     gene            complement(13637..14155)
                     /locus_tag="QF838_01895"
     CDS             complement(13637..14155)
                     /locus_tag="QF838_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024273.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HJO59478.1"
                     /translation="MTPEEQRVREYLISQGEKYPFKQLWTRVIAARLELIKEVSDVTQ
                     SQADFSFHKDEWAISEILEHVINSTSRVTDLISDLTSGGIGDSSGIDPPRELSHSNID
                     ELRGQILEGAFKWTAMTDNLPDTPNMENNSDHGFFGDLHAGGWYMFQRVHDLDHLNQI
                     RNNKSHADYPNN"
     gene            complement(14177..15394)
                     /locus_tag="QF838_01900"
     CDS             complement(14177..15394)
                     /locus_tag="QF838_01900"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59479.1"
                     /translation="MRKILERAFDSLNVRGFRFMWLGDLALSTAEFMEILILSWYILA
                     QTESPLVLGIFAALRFTGTVVAPFFGVIVDRIGRRRIYLFARISFVILSSSIFLLTVF
                     NFLSVAAVLIIAALVGLSRSLDMIVRQSVLPVIVSPPQLQNGVALLRTGRDMTQIVGP
                     VFGGFLLESVGVGRSYLFIIVLHLLSLLFVFLIPGVPNTAIKTAQSMFENLKGGFSYV
                     KINPFLFGLLAVAFTVNLTAFPLNNTLLAVIARQVMNIDAAGLGWLMGTYSAGALLGS
                     LLIGYFSNMDRAGWAMIVGSILWHIGILIMAYMQWFYVSLPVLFFVGISQSFAMVTMA
                     MMLLKYTSAEMRGRVLGLRQLAVYGLPVGVLISGFIAENSNASLALIFNGLLGLFILV
                     LAIFKWPEMLHRR"
     gene            15541..16092
                     /gene="msrA"
                     /locus_tag="QF838_01905"
     CDS             15541..16092
                     /gene="msrA"
                     /locus_tag="QF838_01905"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HJO59480.1"
                     /translation="MSQIEKATLAAGCFWCIEAVFELVEGVNQVVSGYIGGTVENPSY
                     EEICTGTTDHAEAIEVYYDQTLIDYQTVLEIFFSYHDPTTLNRQGADVGTQYRSAIFF
                     HNETQKQFAEQAIKYLNEEKVFPDPIVTEVTHASKFYEGEDYHQSFFKNNPYQPYCQF
                     IIAPKVTKLREKHAHLLKIAQPH"
     gene            complement(16110..17420)
                     /gene="cofH"
                     /locus_tag="QF838_01910"
     CDS             complement(16110..17420)
                     /gene="cofH"
                     /locus_tag="QF838_01910"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HJO59481.1"
                     /translation="MSDIEAHDMDMDFSGLLGSIDPDISRVLRAAISGEDISASDACL
                     LLDSNGLEYNATVLVADVLRRRTVGDVVTYVVNRNINFTNVCIKRCGFCAFSRDFREE
                     QGYLLPVEEIVRRSKEAWDYGATEVCVQAGLPPQMEGDLYIRLCEAIKAELPDIHIHG
                     FSPEEVLYGSIRSKTSIRQHLSDLKDAGVGSLPGTSAEILDQALRDKISPGRITKDQW
                     IEVITTAHDLGIQTTSTVMFGHVETNLQIAEHIDIIRNIQMETGGFTEFVPLSFVNSE
                     APMFLKGLIPNVRTGPTGMDVVKVHAVARIMLNNWIPNIQASWVKEGDRISQLLLTAG
                     VNDLGGTLINESISTSAGAQHGQLMKPSQFRDMIRDAGRLPAERFTNYSLRKVYDKSD
                     NEKDPLDLLEDNVEEIFGSYNKLIGMENFRFEHPRKAAERTASV"
     gene            complement(17413..18615)
                     /gene="cofG"
                     /locus_tag="QF838_01915"
     CDS             complement(17413..18615)
                     /gene="cofG"
                     /locus_tag="QF838_01915"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HJO59482.1"
                     /translation="MTQHKINTHLTDVIAKASEISEITDEEALLLSRYSEDHLGDLCG
                     AAAKIRNHGKGTTVTFSPKVFIPLTRLCRDYCGYCTFRIDPDQAQSLYMSAVEVLDVA
                     KQGENLGCTEALFTLGERPEQKYPEAKEWLSNNGYDSTLDYLGAMTELVVKETRLLPH
                     ANPGTMTHRELNVLKDSNPSTGIMLESTSELLYQKGGPHEFAPSKRPIVRLKTISIAG
                     ELRIPFTTGLLIGIGETMLDRIESLLEIRSLHRKYNHIQETIIQNFRAKPETPMAESP
                     DAETQEMLWTVAAARIILGPQANIQVPPNLSSENYEMYLAAGINDWGGVSPLTIDYVN
                     PEAPWPLITNLKSKTESAGFELRPRLAVYPEYFLDTTEYLPVDLLTKVRELADDEGYV
                     KEGINRYV"
     gene            complement(18633..21041)
                     /locus_tag="QF838_01920"
     CDS             complement(18633..21041)
                     /locus_tag="QF838_01920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59483.1"
                     /translation="MAPFSNLKVLDLSADFAGSYCSKLLSDFGASVKKIIYEGREDRS
                     ALYGPFINEIRDPKSSAMFHYMNYNKQVIPVSKETQTIEELALSADLLIEDFSSEDFN
                     LTGLDLENIRKLNPDILICSLTPFGRNTKWTGKPWSDLTIQALTGMCSVNGEQGKAPL
                     KEPGTESEFITGANAFVASVASLINRDVTGIGQEVNVSILKSVLHSYSPYLLGALHTR
                     NPRGQQPQGLHFGLIPCKDGYVSLSVRHEPTWEHMWLFFGDPEFSENPKFDTAAKRRN
                     NEDELSEILLPILAQYSRKDLLHGLSPLRILVGIANSVSDLLEDEHLKTRNAFVRYGS
                     EGKSWEMPGPPFQMSLTPWKFEPLPTSEDFQIWDNPMKVTHNTSKSLDTGNKTKGPLS
                     GMRGIVLTQAWAGAYSTQLLSDLGMEIIQVESITRIDPWRGGVPPRLAGLYPDTNPGD
                     NPWDRNALYNGVNRGKKAITLDLAQSEAKDVFMELVSISDVVVENFSGRVISNLGLNY
                     ESLRSVNPSIVMVRMPTYGTSGPYSNYPGNGGTTEPVSGMSFLMGYHDGPPINSGIMH
                     TDAYSGVLACGAAITALRERLMSGVGQEVDISQQEVSMTLLAEYIMEGSISGESPGRQ
                     GNTNRDYAPQGCYLSKDSYWIAISVKTDRQWESFCNSLMLDDLITDEDYSTKDVRKIN
                     SVRLDQEISKNMANLVASEVVENLQAESVPCEFISTLLEAAENDDFHQMELFEQIFHL
                     KSGNFYYPVPPWDFENNSRKPQEPAPTLGEHSDEIFSSLNGWSESEIARFQSLGLTGT
                     DPLI"
     gene            complement(21047..21868)
                     /locus_tag="QF838_01925"
     CDS             complement(21047..21868)
                     /locus_tag="QF838_01925"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HJO59484.1"
                     /translation="MAYTDLGLKQDGAVLKITVDRPEVLNAQSRIMREELDDAFDKAA
                     DDESVKVVIIAGAGSNFSAGHDLGSPQEMADREKRPYQPGTAGEYERSWQQNVANTFR
                     WRDFPKPTIAQVQGVCIMGGIILASSCDLIVASEDARFSDRTVRWGGPHVQYLSLPYE
                     IGVRKAKEYLFTGDWMTASEAEKMGLVNKVVPIEQLEEETMKLAQRIALQDAFALRLS
                     KASLNQVQDQMGYRNSIMSGFHNHALGLSYRREIYDGERVTSIDRARERDKKFGD"
     gene            21907..22851
                     /locus_tag="QF838_01930"
     CDS             21907..22851
                     /locus_tag="QF838_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO59485.1"
                     /translation="MDYTIECNDPICVLISEIKLIAIKTNKLTKKYGNSLAVNGVSLT
                     VNVGEVFALLGPNGAGKTTTVEILEGHRTKTSGEVKVLGMDPSNGSTEFRSRVGIVLQ
                     ETGIEQYLTAYEILEQFTNFYKTPRKISDLLEMTGLTEEKDKRVKKLSGGQKRRLDVA
                     IGLCGNPDLLFLDEPTTGFDPSARRTFWDMIKKLKTDGTTVVLTTHYMEEAEYLADTV
                     ALMNHGELITQGSLSDLRISHNKTFITFKTDEPQLQLPKDIKEIAVMSEGAVSISTEN
                     PTAVLSELTGWAISQGIELSNLEVSRQNLEDIYLGILK"
     gene            22848..23627
                     /locus_tag="QF838_01935"
     CDS             22848..23627
                     /locus_tag="QF838_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59486.1"
                     /translation="MNFRKHLPLFLKQIIYESKSYRRNPAAAFFTLVFPLLFLFMLRI
                     FGNDSVEIDGGIVSRATKYIPMITAFSVIGSSYTGIAMTLTVNRDIGVLKRYKGTPLP
                     LILFLLGKIAHNTIVSFALAVIIAIIAFVVFDVGLPTNSIVAFLLTMILGAATFTSLG
                     IAFTSVIPNSDAAAPMVNASVIPLMFISDVFIPMDSAPNWLNGIAQVFPVRPFSLSLQ
                     ETYSPFSSGLTSNPENFAILFAWLVVGVIASLKFFSWEPRT"
     gene            23752..24948
                     /locus_tag="QF838_01940"
     CDS             23752..24948
                     /locus_tag="QF838_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HJO59487.1"
                     /translation="MTALKDLKVLDLSRILAGPLGTQILADLGAEVWKVESPWGDDTR
                     GWGPPFVGDQSAYYLSANRGKKSIIVNLRDEKGQKIIKELAKKADIFVENFKVGNLAR
                     FGLDYESISSLNPKIIYASVTGFGQTGPRAPEPGYDAALQGMTGVMSVTGETEGGPTK
                     VGAAWIDIMTGLTSTIGILSALHERSLSGKGQHVDVSLFDVGIAGMANIAQSYLASGE
                     APGRIGNAHPQVAPYQDFTASDGDFMAAANNDDQFRRMCETVEAPDLAEDSRFLTNAD
                     RVKNRKELERLLNTQFVQKSKSHWIPTLQEAGLTVSSINTLDEVFKDPQAKARKSLWQ
                     IDHPTLGTIDLLGSALQHLSRTPAQPTNHPPLLGEHTEEVMKNVLGMDAQEIQELISS
                     EVIKGK"
     gene            25018..26199
                     /locus_tag="QF838_01945"
     CDS             25018..26199
                     /locus_tag="QF838_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO59488.1"
                     /translation="MKIKSIKAIAVNLSPNPKTQPRVELDPNGGYFVNPMDRYKYDSK
                     YRSGSNWNRVACVVTAEDGTWGLGTTIHSGPVVSIINDHFSELLVGQNPMATEKIWDM
                     MRRASSPYGTAGVVSYAISALDNAIWDLKGKLLETPVYELLGGPVKDDIFCYASNTML
                     RYKTSDYMDWFLELGFKAVKVFLRHGPDEGLAGLNKNVEIIANTREQIGPDIELAVDS
                     WMSSNIEYAVRVSEALKPYNIKWLEDYMLPEDMDSYSKLRQRVPSQILATGEHWYTIH
                     PFADAAGRGLVDILQPDVQWVGGITASVRICHIAEAHGLTVIGHAGMNYPYGQHLAYS
                     MPVIPWGERSEGVSAPGVPLEEMVALPGTPVIKNGRVKPSDAPGFGIEVDIAWIEERA
                     L"
BASE COUNT         8047 a         5889 c         5143 g         7128 t
ORIGIN      
        1 ctcacaaggt gcaagatctg gtctttcacc aggctctgtc aaatcaatga agcaacctat
       61 accacattcc ataagattcc caattttgta tgatggggtg ttcttatccc taaacttggc
      121 tttaaacata ccggaaacag aattcacaat agttgctata gtggtggacg gtctgaatga
      181 ccactgatta ccgggatatt ccccagccgc caatttaccc tggctaaccc agtaagcaaa
      241 aacattcaaa tcattgttag gattatcaaa catgagtata aaattactcc gtacaaccta
      301 tatattaaag aacaaaattt gtacgccgaa ggtgcattaa tggtatcaaa attggacaag
      361 gacaataatt tagtctggat agatcttgaa atgactggtc tggaggatga ccacttaatt
      421 atagaaattg catctatcgt gactgatggg aatctgaaag agctagcaga aggcccatct
      481 ttagctataa atcgaactat atccgagatg cagaacatca atgattggtc aaaagagcag
      541 caccaaaaat caggactact taaacgggtt agggaatcaa cagtgacggt atctgaggcg
      601 gaaaatcaaa ctctcgcatt tttaaaggaa tgggtgtccg ttggtatgtc tccgatttgc
      661 ggcaattcta tcggtacaga tcggagattt ataagaaggg aaatgccaca gctcgacatt
      721 tttttgcatt acaggatggt tgacgtatca accataaaag aactagcaag aaggtggtat
      781 ccaaagacga atctgcctga aaaagaaggc aaacatcttg cattagagga tattcgagaa
      841 agcattgaag aattgagatg gtacagaaag acgtttttca atattgagat ttgagtctaa
      901 ggagattcaa aaatttgata aaaagaatac ttggaaaaac cggattggaa gtttcattat
      961 tgagcctggg tacaggtggt gcgagacaac tcggtcaaac aatcgggcat tcgatatatg
     1021 atcaaaaaaa actggtggaa gcagcgcttg ataccggcat caatattttc gacactgcta
     1081 cccattatgg taattcagaa tctatattag gaaaatgttt gtcagacaaa cctcgtgatt
     1141 catttatttt gtgtaccaaa tggccagcca gagatgaact gaacaacacc attgatgacc
     1201 ctaccaaatt aatcaaatcc gtagaaaaaa gcctagatag gcttcaaacg gactatatag
     1261 atataatgct tttccacggt attatgcctc aagaatatga cctaatcatt gaaacacttt
     1321 atccaaccat ggaaagatta aaatctgaag gaaaaattag aagtatcggt tttagtagcc
     1381 gcttttctga agatcccgca caagagggta tcaaattagg tttatcgaaa catccggaat
     1441 tgtgggatat cgttatgctt aaatatggga tcttgaacca atctgcaggt gaaaacatat
     1501 tgcctttggc ccgaactcac aatattggag taattaacat ggctgcggtc agaataaaac
     1561 tgcctgatcc tattctttta gagcaattga tcgctgagtg gaaatattcg gggttaatag
     1621 ataaggactc tcttcctgcc catgatccgt ttggatggtt ggttgaggga aatgtgaaat
     1681 ccgtgataga ggccggttac aaatttgctg ccgaaccaac aacagtttca acggtattga
     1741 cagggacagc ctcaattgaa catttaatgg caaatgtaga atgtcttgaa caaccatatt
     1801 taccgcaaca tcacacggat cgacttaaaa ccactttaag ccacatcatc caatacgcat
     1861 aatatacatg ctgctaataa attaaaaaat taggaagaca ggacatctaa tatagtggat
     1921 gaaaatacaa atcaagattt cattagagac attgtcaaag aagatttggc ctcaggcaaa
     1981 caccaattta tcacaacccg atttcctcct gaaccaaacg ggtacctaca catagggcat
     2041 gccgcatcca tttgccttaa ttttggaata gcaaaagaaa atgaatccgc aaagtgcaat
     2101 ctcagatttg atgacactaa cccatcaaaa gaaaataccg aatttgtcga agctatcaaa
     2161 aaggatattc agtggttagg gtttgattgg gacgagcgtg aatattttgc ttcagattat
     2221 tttcaaaaac tttatggatt tgcaatgact ctcataaatc agggtaaagc ctatgttgat
     2281 agccaaaccc aagatgaaat tagagcaaat agaggaacac tcactgagcc cggggcagat
     2341 agcccatacc gggacagaaa ccctcaagag aatacaaagt tgttagagga aatgcgtaac
     2401 gggattcacc ggcctggttc acatgtcttg agagcaaaaa tagacatggc ctcccccaat
     2461 ctaaatatgc gagacccggt aatgtatcga atcttagata cacctcacca tagaactggt
     2521 aatgaatggc ccatatatcc tatgtacgat tttgcacacg gtctgtcaga ctcaattgaa
     2581 gaggttactc attccttatg taccatggag tatgaagatc acaggcctct gtacgaatgg
     2641 ttcatagatg agcttaaagt attcaaatct agacaaattg agtttggaaa aattttcctg
     2701 agccacacca tactaagtaa gcggaattta actaaactgg tggaaaacaa attggttagc
     2761 gggtggaatg acccccgaat gcccacaata tctggtatga gacgactcgg ctacacagca
     2821 gattccataa gggaattttg ccgtagggtg agtataacta gaagatggaa tacggcagaa
     2881 atagaactct tagaacattg tttaagagaa gacttaaata aaaaatcaaa tagagcactc
     2941 gcggttttac ggcctatcaa attagtaata gaaaactacc ctgaagatca ggtcgagtac
     3001 cttgaagctg ccaataatcc tcaagatcgt gacagtggga caagaaaaat accattttca
     3061 aaagaactgt tcatcgagca agacgatttt atggaaaacc ctcctaaaaa attctttcga
     3121 ttaactttag gaagagaagt taggcttcga tacggttact taatcacctg tactgaaatt
     3181 attaaagatc ctataaccga cgaggtttta gaactaagat gcacttatga cccagaaaca
     3241 cggggtggaa atgcaccaga tggaagaaaa gttaaatcaa ccatacactg ggttagtgca
     3301 aatcattcga ttaacgctga ggtgcgactt tacgaaagac tttgcctaga ctcggagcca
     3361 gatatatcgg atggggaaaa tttggaaacg gttctgaatc caaattctct cgaaattatt
     3421 cggtctgcga aagtggagcc aagccttaaa gaatcaaccc cgggaaaccc aatgcaattc
     3481 gaaagactgg gttatttttg cttggattct gaggattcca aaaacgaagg caaccttgtg
     3541 ttcaacagaa cagtttccct tagggatagt tgggccaaaa cccagtcaag taattagata
     3601 caggacatca ggatctactt tgattgggat ttttgcaaaa aaggaagccc acactaccaa
     3661 ctaacgctac cgaggctaga atataaaaag cccgatcgta gctgccgtag tgatcaaaca
     3721 taaatcctgc cacccatggc cctatcatca tcaatgtcat catcggtagc atggacacgc
     3781 ctaaaactct cccaaatgct tttcgtccga aatattctcc ccttatcgct gttccaagtg
     3841 gaattctggc tccaaaacct aatccatata taaaaacaaa tatagaagcc atgtatacac
     3901 tagtaataaa tgttgcatat atgacaccaa gcgcctgtat tatcccaaaa acacaaagag
     3961 ataagttctt aggaactcta tcccctagat agcctccgag aacttgggcc gctccagaaa
     4021 aaagcatagt ggctccccat atccaggctc cggtttgaac tgatattccc tgatccttta
     4081 atgccaaaat cagatgtaca gtcatcgttc caataagcat cgcagatgtg ccattacaaa
     4141 ttgccaaaac ccaaaacacc ggcatcttta taatcgtcat cataggaaag tcattgtcga
     4201 ttcttgtagt tacacttcta tctgaatctt ttacctcaaa aacccttccg tctggaattt
     4261 cgcctaattc ctcaggagaa tttcgtataa gcctactaat gggaaaggct atgagaatga
     4321 aaaatatccc caaagacaat gaagtagttc tccagccaac accctctggc tgaaccagcc
     4381 aagccaatag tggtaccaaa aaaaagctgc ccactgaaaa accaataccg cctagtccca
     4441 ttgccattgt tcttttctta tcaaaccagt tgttgataac agacatcatc ggtagccagc
     4501 ctccagctga ggctcctaac atgatgacaa tatatgcaca ataaaacgtc caaaggttat
     4561 tggtttgact gaatatgagg aatcccaaac caataattgt cacaccagag aacacaatct
     4621 ttttaggacc atagcggtca actagaattc ctaccactgg gccagccagg ccactttcta
     4681 gctgaccaaa ggaaaatgcc aaagaaagct ccgccctact ccagccgaaa tgctgttcca
     4741 aagcatcgat ccatacaccc actccaccaa aaacagggga ccctgtcgca gcattcaaaa
     4801 aaatggttat aacaaccaac caccacccat aaaagacagc gtggcgccat ttaaacggtg
     4861 aaaataaggt aggcgattca gttaatttca tagactccaa ttcagatccc gacaattaca
     4921 aacccaatat atccaaactc agaaatattg gtggtaagtt aatcggaatt catgaattta
     4981 aataatgtaa taaagcgccg attgggtcaa gtatatttaa actaccttgc aggcggggtt
     5041 gcctctggct cacgaacata tggtaccaac cgaggagaat caaattgggg ttcgcctttt
     5101 atagattcca ctaattcaaa agcctttaaa acggtatcgt ggtcaccttc tagagccaac
     5161 atgactgcac cttcagaacc tgcaacgcca ccagaaccaa catgagtagc agtcactccg
     5221 gtcaataact ccaaagcttg tatttccgtg atcacctgag cgttcacaac aggcataaac
     5281 ccagccgatt gtcccatcgt ataatcccat aaatgatttc cacagtgttt ggcagcctcg
     5341 atcaccgatg gtatcaacct ttccaaacta acaggcgcca cccaatgggc acccctggcg
     5401 gtgagagttg gccaaatacc ccccacagta ccaccggcct tgtcggcagc aaaaacgcct
     5461 atattcccct cggaatcaac ggcattggca ccttttattg acacgtcata tgcagtaaat
     5521 tcgcttaatg cttgaggagc gggtgtttca tctagttggc catctttgaa aacccacggt
     5581 ccgagccggt cttcttccaa aacactcgcc attcggcctt cagtaataat cccagcggta
     5641 caattggcgg tatcaaattc ctgacctgtc aactcctcca atatatatga aggtgttata
     5701 cccctggaga ttacaaacca tccttgtttt agaactctct ccacttcggg cattgccaag
     5761 acccctttag caataaggcg cttagattct ctgggaagaa tatttaccaa agcgtgtgtc
     5821 ctgtcatccc tcattggctc caagggttct ctatatgaat gagtatcgta aaacatatgt
     5881 acctcttaat tgaattaata aaaaaaaccg cgtaaataat aactcaaata aatatacatt
     5941 gtgagaagac atgttagttt tagtcagtta tactatcgct cgataaaaga ggctaataac
     6001 acaaaatgga ggtatagagg ccatggtgga acaacagtta cagatactac gacctgagac
     6061 tctcgtagcg gagttcaaga gtatcggtgt tacgcatatc attacgatcc ctgatagtga
     6121 aacaaattat ttatatgaat taatggaaaa agaagattgg ttggatgtag tacctgtatc
     6181 cagagaagga gaatcaatgg ctgtcgcctt gggactcaat gttgcaggca aggtaccggt
     6241 ggttcttatc caaaatacag gcatgatgga atctggagat tcgatcaggg gaatggcgtt
     6301 ggatgccggt tttcctcttc taatggttgt tgggtatcgt gggtggactc ggaaaggaca
     6361 cacgccagac tcagctgccg tttatacaga accatttctc aacgctttcc acctaaatta
     6421 ttatttggtt gaaactgacg acgatggagc tcgaataact gatgcctata acgaagctgt
     6481 taagacaaaa agaccagtgg tcgtactaat tggagatgaa tatcacgggt ttaatagata
     6541 atcgggtttt aaatcgtcag gagtataaaa tgatcgaatc aaaagaagta tttgaagcat
     6601 ttcaagaaca caggggcaat gcaattgtgt cggtccaggg tacgtctgga aaacattggg
     6661 ccgatgtaac gaccaaccaa aatagggaca tctctctggg tggggccatg ggccagtcca
     6721 ccgcagcgat atttggtttg gccttgggcc ttccaaacga caaggtggtt cattttgata
     6781 ccgaaggagc attgctaatg aacctcggtg ttttagcctc aatagccggt aaaaaacctg
     6841 cgaattttgt tcatttcctt ttggacaatg gatgttacgc cactactggt ggtcaaccgg
     6901 tgcctaactc cgaagatatc gactattcag taatagctgc tggatcaggc tacgctgcca
     6961 catataattt cgacgaccta gaggaactct ccaccagcct agacgaaatt atgaaccaag
     7021 atggcccggt ctttgtagcc attaaagttc cggctgaagt tgaaaatctt cctataggaa
     7081 tgcgagaaag aagggtatca aggaacagag ggcaaacgat atctgatttg agatcggaac
     7141 taggaataag ttaaacaggc cctaataatg gtgagggtgg gtacaaagtt taaagaaggt
     7201 tcccattcct ctaaacccca gtaatagcgt agcatttgga acattggcta aataggctat
     7261 aactctgcca attccctaat tatgtaccta tttattaggg ggtttggacg atttattctt
     7321 cgcccagagg cagcaatcgc acagtacaat ggccgcctga aaacaaaaaa tttgaatcgt
     7381 acgcgatgta acgaggtacc aatgcccgaa ttcaagaaca tactttatga aatagacgga
     7441 ccagtcttaa ctattacttg gaaccgacca gacgcactca atgctatttc tcagaatctc
     7501 gagcgcgaat ttcatcatgc gcttgatatt gctgcagaaa acgaagcaat ccgatccatt
     7561 atcgttaccg gcagtggacg tgctttctca gctggctacg acatctcggg tgacagaagc
     7621 aaacgacctc gtcttccaac aagcactcat ttaaagcggc aatggggttc atcgatgctc
     7681 agcacgggcg gacctacgca catcatgcat ctaatggagc atccaaagcc catcatcgct
     7741 gcagttaacg gctggtgtat tggaggaggc ttctggtact cacttgcttg cgacatcacg
     7801 attgcttcgg atcaggcgac tttcggtcag ccggagattc gaatgacgtc aaatacaact
     7861 gttttgtttg cactgctctg tggatggaaa aatgcgcatc gatacgcgct aacaggagat
     7921 catttcgacg cgcaagaggc actgcgcatt ggagcgatca acgaagtggt accgcatgac
     7981 caactgttag aaaaagctcg agcactagca ttgcgacttg caaaagtgcc gtctgaatcg
     8041 atccgattca acaaggcgat caccacatac ggactcgatg caatgggtct acgttctgct
     8101 ctaaatatga atggtgcgct ctccacaatt gttgaggctg cgaacgacgg tccggatgtt
     8161 gaacaccttg atcgcgcgca ggcagaaggt agctttggtg cattccttaa ggcgcgggat
     8221 gaaccattcc taccggaacc gttcggaccg cgaagcaagc ctcgtgattg aagatctggt
     8281 taaaatcgga gcatgttttg gagtacatgc ctccttccat ttatcactcc cctagacaag
     8341 agctatccca taaaacaggg atcaggatcg acaacatccg acctagtgcc cgtgatcaca
     8401 gtgaaatatg gtcgaatgct ctcccatggt tgaagaaagg tgtaggcgta ctttgacctg
     8461 ccaggcctag cgtgcagcaa acatgcagct atgcccttga gtttacctgt aattagttaa
     8521 gtgcttcatc agcgtttgcc cttgtaaata ctgatgagtt gggactaatt gaaccctata
     8581 aaaagaaggt gctgagaatc taactcctgt aattggggtt atctagcggt accttcatcc
     8641 agctcataat ggccttgact gagttgtaag cttccaacct agcttcatca gttccgtccg
     8701 attcggcatc ttcaaaaata tccgatattt ttcttaccat atcaggaccc aaatccaaca
     8761 aagactcact tcctgacata gcccatttac aaagaatgta cctaactact gatccaatag
     8821 gtatatccag atttcgactt aaccgtctta ctgttgggag aggatcttcc tgagtatata
     8881 gagcaacatc atttttgaaa ttattatcct tatcagaaac atttgattcc caaatgaatt
     8941 gctccaaaat aaccgaatca tcaaaaagca taattttttc tcactttttt aaccaagatc
     9001 ttccttcttc gatatattga gtctgaccaa cagctgatat tgtgatttcc tgcgaatagg
     9061 tcccgaattc tgtacttaag gaagcttcat gtattttcat ttctgatatg atctcgttat
     9121 cagatccttt gaagctttct tctaatattc tcccttctac atgagcaggg agctcttctc
     9181 ccaatattga cagtatggtc ggcagaatat ctgtgttacc agtaggtgac aatatttttt
     9241 caccttccag aaatgtaggc ccactgcata tgagagtatt gttcatctca tgtaggctga
     9301 tggaaccgtg ctgtccaaga ttttttgcac caccagttga aaacacatgc cctgcgtatc
     9361 catcaacgtt atcagaggag tcccagtgga aagacattat tatatctgca gacctttttc
     9421 cttcattcat aatagaagac aaaggaatag taccttttac ttctcctaaa cgttccgaag
     9481 cgcataaggt tccacaccat acctgaccag aaagccattc aaccaactcg ggaatcaaat
     9541 aaacatcttc ttgatttttc aaataaaaaa gcacacaacc accattttgt gccaagagcc
     9601 aaccatcgga acctgccaaa ttagaagact ccagaagtgt ttcaatatct atcacttccg
     9661 taatagttga atacccatga tcggacagaa tcattaaatc cgaattctga tgttggctcg
     9721 aagtactgat gtactccata attctgccga attctgcgtc agcctgcctc agtgctgcct
     9781 gggcagcatc acttccaaca ccagatgcat gctgtgattt atctggctct gaagaccata
     9841 ttaaggcaac ttcaggattg attttaccca acacatactc aataaatata tcgactgcct
     9901 ttttgtatct tggagtgtta ggtaattgtt cttctggcca tccaccgaat aagccttcaa
     9961 gttccttatg caatctgtga ggtattgtga attcaggatg aatggtagct ccaccataaa
    10021 catcagcaag agggttatgc acataagcat ttccactggt gcctacacca atcgcgacat
    10081 actccatact tttttccgaa atgatttctt gcaaagttgg tacacctagc acctccaagc
    10141 cagcatttcg aatctcagat aattgaacgt tgagggccag tatcaccttc gaatcatcat
    10201 aatctcttgc tacaaaagag ttaccagcca accaatgttt accaggattc acgcctgtga
    10261 ccattgatgc agcgtttact ctagtaactg acggaaaaac aggatgatgg ttttcaaaaa
    10321 aagcaccatt ctgggatact cggaacaagt taggtgtatc cacactattt atttgagcgg
    10381 gctgcaaacc atcgaagaca gctaatatta tcttagtcat atttctccct ctaaaaattc
    10441 cggctaatca gaaaaccttg aaattattat cttgagccca tcagatgatc tcctaaaatc
    10501 ggtcgcctgt cactccctat gttattccca ctatacccag ccttgacccc tcttgtcgtc
    10561 aacatatcca atcactaacc tatcaaaatc aaggtctgct acaatcgatc gcactcatac
    10621 tgttcatacg aggaagaaat gactgatagc caaaaacggc accacttcag acagatactc
    10681 tcgggtaatc gatgcgtgca tccaggatca gtgtttgacg ccatatcttc tcggattgct
    10741 gaagacttag agtatcaggt gggtatgttc gccggctcga tcgcctcggc tgtagtgtta
    10801 ggtgaccctg accacattct aataactctt actgaatttg cagagcaagc acgaagaata
    10861 tgtcgtgcct cagaccttcc aataatggta gatgcagacc acgggtacgg aaacgcactt
    10921 aacgtcaaaa gaacagtgga agaactagaa aacgcggggg tggctgcact cacgattgaa
    10981 gatactgtcc tcccaaagca gtttgggaaa tctgagccag agttaatatc tttaccagaa
    11041 ggtataggta aaatgaaagc ggcattgtcc ggtagaaaag atcaaagtct agtaatagct
    11101 ggcagaacca gtgcattcca agtaacaggt acggaagatg ctattgaaag ggccaaggca
    11161 tatgaaagca tcggagttga tgcgattttc tttgccgggt taactaccag atctcaacta
    11221 gaagccgtca agaattccat tgcgatacct ataatgctgg gaggtacacc aaaggacata
    11281 aacgacgacc agtatctttc atcaatggga gtgaagatag cgctccaagg acacctacct
    11341 tttatggcag ctgtaaaggc aactagagac acaatgaaag ctcttattga aggcaaacaa
    11401 ccttcagaag tcagcacaac agcgctagat agtgagttaa tagacaaagt gactaaagac
    11461 caacaattta aagactggtt taaagagttc ctatcctaat cattattcat gaaattaaag
    11521 gtatcacttt cttttcgaac aatttcattg acttaattac ctcttcctga gggagtcctc
    11581 ccatcgcaaa attgcatccg aaatacgaaa ttccgtgatc ttttagagaa atgatttttt
    11641 cagcgacagc ctcaggtgtt cctataaatg ctcttttccc agagatatag cggttatctg
    11701 gtggtaactc atctcgctca ttcctccatt tttttatggg taaatgaacc tcagtagtac
    11761 ccactaagtt cctacgccat tgcataatat cctgtatcca atctacatgt tttttggtgt
    11821 tatcaagagc ctttctctcc gtatcagcca catgaacata ccgaaaaaac ggcacttcat
    11881 ccatatgatc cgattgatcc ttcaggtgac ataatcttct atatttttct accaaagcaa
    11941 gcgcagcatc tgtatcttga acaaccgcta tacataaaag aaactggttt gaaatcataa
    12001 aatccaaggt ctcttcagac ctcgttgcag ctatatatat aggaggatga ggtgactgaa
    12061 caacagatgg aaccaaatcc acattgcgaa cagaaaaaaa agggctgtcg acacttacat
    12121 ctttatcaca ccataaatcc cgtactgcta ggacggtttc tctaaaccta gattggctat
    12181 cttccgcggg aactccaaaa ccaccatatt cataaccata tgtacctctt ccgaacccaa
    12241 catctagacg tcctcggctt aggagatcta caagggctga gtcctcagca atcctgatag
    12301 gattatgcaa gtttggaaca attactgcgg tccccaaacg tattttctct gtcactgccg
    12361 ccaaatgccc aagcattagc aacacagaag aacccatact gtatctagtg aagtgatgtt
    12421 ctgcgaacca tgctccgtgg tatcccatag attcagctaa cttaacctgt tcaactgaat
    12481 catgaaacac cgaagtctga ttcactccct ctggccacga catatgtgtg aaaagtgcta
    12541 gcctcatatc tgttaatcca aatcagaaac aagatccatt agccttccta aagcatcgct
    12601 tttactagca atatcgtcac caggtaaatc aactttaaga gtactaacac ccagattctt
    12661 atagagtaaa agtctttcct taatcatttt atctgttcca attaaattgg atctcaaagc
    12721 aagctcatta ggtaccatat ctcgagcctc ttctcttctc ccttcaaccc acaatttttg
    12781 gacaacatct acaatctcgc cataaccttg tcttgcatac gcattcttgt aaaaattgtt
    12841 cttcactgac cccattgccc caaaggtaaa tccatatcca cgagcatgtc tttttgctgt
    12901 ttcttcaacg tcatcggtga aatctaagga aactggtatc aatatatcta tgtcttcaat
    12961 acttctacca gaacgaaaga gcccttcttt gattttatcc agaaaaacgt ttcctgtttc
    13021 tgggataaaa caacctccta tccatccatc ggccttttca ccagtcattt ctaaattgct
    13081 aggaccaaga gaagccacat atactggaac atgccaagtt ttagcgctgg ttcttatgga
    13141 cttaccttct ccgccatcca agggtaattg atagtatttt ccttgatact ggagcttttc
    13201 cccggacaca cccattttga taatttccaa tagttccttc gttcttagta caggattgcc
    13261 aaaagtcacg ccgtgaaatc cttctataac ttgtggccca ctagtaccca gcccaagaat
    13321 aaatctacca cccgacatag attgcaaagt caagctagtc attaccaaat tacctggggt
    13381 ccgactaccg acctgaacaa tccccgtacc caaccgtatc tttgttgtgg aagcagccaa
    13441 ataagctaga ggggtgatcg cgtcggtccc ccaagcttcg gaggtccata tcgaatcagc
    13501 ccctaaagat tcagcttcct ttgcaaaatc tgctatttga ttccaatccg aaggatcaga
    13561 ccaagataag tcagacctaa caccactaaa tgatcgtact gctattttca tgttatctgt
    13621 ctatcacctt tattcactaa ttatttggat aatcagcgtg agatttattg tttcttattt
    13681 ggttcaaatg atctaaatca tgaactcttt gaaacatgta ccaacctcca gcatgcaaat
    13741 cgccaaaaaa accgtgatct gaattgtttt ccatattggg agtatctggc agattatctg
    13801 tcatagcagt ccatttaaat gccccttcca gaatttgtcc gcgcaattca tcaatgttgg
    13861 agtgagaaag ctctcttggg ggatctatac ccgaggaatc ccctatacct ccactcgtga
    13921 gatctgaaat aagatctgtc actctacttg tactattaat tacgtgttcc aatatctctg
    13981 aaatagccca ttcatcttta tgaaaagaaa agtctgcctg actttgagtc acatctgaca
    14041 cttcctttat caattccaag cgagccgcta taactctggt ccataactgt ttaaagggat
    14101 atttttctcc ttgcgaaatt agatactctc tgactctttg ttcttctggg gtcattatct
    14161 ttaatcctct aagcgactac cttctatgca acatctcagg ccatttaaaa atggcgagta
    14221 ccagtataaa caaaccaagt aacccattga aaattaaggc aagtgatgca ttgctatttt
    14281 ccgcaataaa tccagaaatc aacaccccta caggcaaacc gtaaacagct aattggcgaa
    14341 ggccgagaac ccgaccccgc atttctgctg aagtgtattt caacaacatc atagccatcg
    14401 tgaccattgc aaaagactgt gatatgccga caaaaaataa aactggcaaa gatacgtaga
    14461 accattgcat ataagccatg attagaatac ctatatgcca taatattgaa ccgactatca
    14521 ttgcccatcc tgctctatcc atattagaaa aataaccaat aagcaatgaa ccaagtaagg
    14581 ctccagcaga gtatgtaccc atcaaccacc ctaagcccgc tgcatcgata ttcatcacct
    14641 gcctggctat cactgccagg agtgtgttgt ttaatggaaa tgccgttaag ttaacagtaa
    14701 acgcaacagc aagcagacca aacaaaaacg ggtttatttt gacataagaa aatccccctt
    14761 ttaagttttc aaacatagat tgggcagttt ttatagcagt attgggtact ccagggatca
    14821 agaacacaaa gagcaaagat aataagtgca aaacaataat aaaaagatag cttctaccta
    14881 cacctacaga ctccaacaaa aaccctccga aaaccgggcc aacgatttgt gtcatatccc
    14941 ttccggtcct taagagagcg acaccgtttt gcaattgagg cggagaaacg ataactggaa
    15001 gcacagactg tctgacaatc atatccaagg aacgggaaag cccgacaagg gctgcgatga
    15061 ttaaaaccgc tgctacgctt agaaaattaa acacggttaa taaaaatatt gacgaagata
    15121 atatgacaaa tgaaattcgg gcaaacaggt atatcctgcg ccttcctatg cgatcaacta
    15181 tgaccccaaa aaaaggcgct accactgtgc cagtaaacct aagcgcggca aaaatcccca
    15241 gaacaagagg agactcagtt tgcgctaata tataccaaga caagatcagg atttccataa
    15301 attctgcagt tgagagagcc aagtctccta gccacataaa gcgaaatcct cgaacgttta
    15361 gagaatcaaa agccctttct aaaattttcc tcacagaacc attgaaccta ctccacagtt
    15421 actgaacatc agagaatcct acacgaatct ctaacctaat acatatcatt taccataagg
    15481 gtataatccc aatcgaagaa acgtgaatta gtccgaaata gacgtaaggt ggtaaaaagc
    15541 atgagccaaa tagaaaaagc tacactggca gcaggatgct tttggtgtat agaggctgtt
    15601 ttcgagttag ttgaaggagt aaatcaggta gtatcaggtt acataggggg aacagttgag
    15661 aacccatcct atgaagaaat ttgcacaggt actacagacc atgcagaggc aattgaagtt
    15721 tattatgatc aaactttaat tgattatcag actgtcctag aaatattctt cagttaccat
    15781 gatccgacga cactaaacag gcaaggggct gatgtgggga ctcaatacag atctgccatc
    15841 ttctttcaca acgaaaccca gaaacaattc gctgaacagg caataaaata tctaaatgag
    15901 gagaaggtat ttcccgatcc aatcgttaca gaagtgacgc atgcctcgaa attctatgaa
    15961 ggtgaggact atcatcaatc attctttaaa aacaatcctt accaacctta ctgccagttc
    16021 ataatcgcac cgaaggtaac gaagttaaga gagaagcacg cacatctatt gaaaattgcc
    16081 caaccacatt aattcaaatt agcctttcgt catacactag cagtcctttc tgcggcctta
    16141 cgtgggtgtt cgaatcggaa attttccatc ccaataagtt tattgtacga gccaaatatt
    16201 tcctctacgt tatcttccaa tagatctaat gggtctttct cgttatccga tttatcatat
    16261 acctttctca aactataatt tgtgaatctt tctgccggca accttcctgc atcccgtatc
    16321 atatctctga actgtgaagg cttcatcagc tggccatgtt gcgcccctgc ggatgtagaa
    16381 atactttcgt taatcaaagt gccacctaaa tcatttaccc ctgcggtaag aagtaattga
    16441 gagattctgt ctccctcttt aacccaagag gcctgaatgt ttggtatcca attattcaac
    16501 ataatcctcg caacagcatg aaccttcacc acatccatac cggtgggtcc agtcctaaca
    16561 ttagggatta gccctttaag aaacataggg gcctcagaat tcacaaaact caatggtacg
    16621 aattctgtga acccacccgt ttccatctgt atgttccgaa taatatcgat atgttcagca
    16681 atttgcagat ttgtctcgac gtgcccaaac atcacagtag atgtagtttg tatgcccaga
    16741 tcatgggctg tggttatgac ctcgatccat tgatctttag ttatacgacc cggtgatatt
    16801 ttatccctca aggcctgatc caatatctct gctgaggtgc cgggtaaact tccaacacct
    16861 gcgtctttca aatcactaag gtgttgcctt atagatgtct tggatcggat tgatccataa
    16921 agaacctctt ctggggaaaa tccatggatg tgaatatcag gaagttcagc ttttatcgcc
    16981 tcacatagtc gaatgtataa atcgccttcc atttgaggtg gtaggcctgc ctgtacacat
    17041 acctcagtag ccccataatc ccacgcttct ttcgagcgac ggactatttc ctccactggt
    17101 aatagataac cttgttcttc tctgaaatct ctgctaaatg cgcaaaatcc acatctcttt
    17161 atacatacat tagtaaaatt aatgtttcta tttacaacat atgtgacgac atctcccaca
    17221 gtccttctac gcaaaacatc ggcaaccaac acggtagcgt tgtactctaa cccattagaa
    17281 tccagaagca ggcaggcgtc cgatgctgaa atatcttcac cagaaatagc cgcacgtaag
    17341 acgcgggaaa tatctggatc tattgacccc agcaggcccg aaaaatccat gtccatgtca
    17401 tgtgcctcaa tatcagacat atctgttaat accttccttt acatatcctt catcatcagc
    17461 caattctcgt accttcgtca acaagtctac cggtaagtat tcagtagtat caagaaagta
    17521 ctctgggtat acagctaacc ttggtcgcaa ttcaaatccc gctgactctg tttttgattt
    17581 caaattggtt atgagaggcc agggtgcttc gggattaaca taatcgattg tcaaaggaga
    17641 aacaccaccc caatcattta tacctgcagc caaatacatc tcgtaattct cggaactgag
    17701 gtttggcggc acttgtatat tagcttgagg ccccaaaata atacgagccg cagcgaccgt
    17761 ccagagcatt tcttgtgttt cagcatctgg agattctgcc atgggggtct caggttttgc
    17821 cctgaagttc tgaattattg tttcctgtat atgattatat tttcggtgta ggcttctgat
    17881 ttccagtagg gattctattc tgtccaacat cgtttcacct atcccaataa gaaggccggt
    17941 agtgaatgga attcttaatt cacctgctat tgaaattgtc ttcaatctaa caataggtct
    18001 tttactaggg gcaaattcat gaggtccgcc tttttgatat aaaagttcgc tggtactttc
    18061 cagcattatg ccagtagagg gattagagtc ttttaaaaca tttagctctc tgtgagtcat
    18121 ggtaccggga ttcgcgtgag gcaaaagcct ggtctcttta actacaagtt cagtcatggc
    18181 acccagataa tcaagagtac tgtcataacc attatttgaa agccattcct tcgcttccgg
    18241 gtacttctgt tctggccttt ctccgagggt aaataatgct tcggtacagc ctagattttc
    18301 cccttgtttg gccacatcaa gcacctctac tgcactcatg taaagacttt gcgcttggtc
    18361 tgggtctatt ctaaacgtgc agtatccgca gtaatcgcgg cataacctgg tcagagggat
    18421 aaaaactttt ggggaaaaag taaccgttgt cccttttcca tgatttctaa tctttgctgc
    18481 agcaccacac aaatcaccca gatgatcttc tgagtagcga gaaagtaaaa gtgcctcttc
    18541 atcagtaatt tcagaaatct ctgaagcctt agcaattacg tcagtcagat gtgtatttat
    18601 tttatgttga gtcatcattt cacttaagta ttttaaatca aaggatctgt accagttaat
    18661 ccaaggctct gaaatcgggc tatttcactc tcagaccatc cattgaggct actgaaaatt
    18721 tcgtcagaat gctcccctaa tgttggggct ggttcttgag gttttcgaga attattttca
    18781 aaatcccaag gaggcactgg gtaatagaaa ttccctgact tcaaatgaaa tatctgttca
    18841 aataattcca tttgatgaaa gtcatcattt tcagcggctt ccaataaagt ggaaataaat
    18901 tcacatggca ctgactcagc ctgtaaattt tccacgactt cacttgctac caaattagcc
    18961 atattctttg atatttcttg atccaaccga accgaattta ttttcctaac atctttggtg
    19021 gaataatctt catcggtgat caaatcatcc agcattagcg aattacaaaa agattcccat
    19081 tgacggtctg ttttcacaga aatggctatc caataagaat cctttgaaag ataacatcct
    19141 tgaggagcgt agtccctgtt ggtattgccc tgtcttccag gagattcccc tgatattgac
    19201 ccctccataa tatattcggc caataatgtc atgctcacct cttgttggga aatatctact
    19261 tcttgaccca ctccagacat taacctttcc ctcaaggcag ttattgcggc accgcatgct
    19321 aatacaccgc tataagcatc agtatgcatt atgccagaat ttataggagg tccatcatgg
    19381 tatcccatga ggaatgacat gccggagaca ggttctgtag ttcctccatt accagggtaa
    19441 tttgagtaag gtccgcttgt cccataagtt ggcattctaa ccatgacaat tgagggatta
    19501 accgacctaa gagattcgta attcagtccc agattactta ttactctacc ggaaaagttc
    19561 tctactacga catcacttat tgaaacaagt tccataaata cgtcttttgc ttctgactgg
    19621 gctaggtcaa gagttatagc tttcttacct ctatttacac cattgtaaag agcatttcta
    19681 tcccaaggat tatctccagg gttagtatct ggatacaagc ctgccaacct tggaggaacc
    19741 cctcctctcc aaggatctat ccgggtaata gattcaacct gaataatttc catgcctaga
    19801 tctgagagca gttgggtgct gtaggctcca gcccaggcct gtgttaacac aatccctctc
    19861 atcccagaaa ggggtccttt tgttttattg cccgtatcta aacttttaga ggtattatgt
    19921 gtgactttca ttgggttatc ccaaatttga aaatcttccg aagtaggtaa gggttcaaat
    19981 ttccatggtg ttaaagacat ctggaaagga ggtcccggca tttcccatga tttgccttcg
    20041 ctaccatatc ttacaaaagc attccttgtt ttaaggtgct catcttccag taaatctgat
    20101 actgaattgg cgatgccaac caatatccga agaggactca aaccgtgaag gagatcctta
    20161 cgagagtatt gcgcaagtat tggcaaaaga atttccgaaa gctcatcttc attattacgt
    20221 cttttagcgg ctgtatcgaa cttcgggttt tctgagaatt cagggtcccc aaaaaatagc
    20281 cacatatgtt cccatgtagg ttcatgacgc acactgaggg atacataccc atctttgcaa
    20341 ggtatcaggc caaaatggag tccttgtggc tgctgtcccc gagggtttct ggtatgaagt
    20401 gcccctaaca aatatggact ataactatgt aaaacagatt tcagaataga tacattcact
    20461 tcttgcccaa tccctgttac atctcggttg attaaggaag ctacagaagc tacgaatgcg
    20521 tttgctccag ttataaactc actttccgta cctggttcct ttaaaggagc tttaccctgc
    20581 tctccgttta cactgcacat acctgttaaa gcctgaatag ttaggtcgct ccaaggtttt
    20641 cctgtccatt tagtatttct cccaaagggg gtcaaagagc aaattagaat atccgggttt
    20701 aatttacgta tattttctaa atctaaccca gttaaattaa agtcctcact tgaaaaatcc
    20761 tcaattaata gatccgctga aagtgccaat tcctcaattg tctgtgtctc tttggatact
    20821 ggaataacct gtttgttgta attcatataa tggaacatcg cgcttgactt tggatctcga
    20881 atttcgttga tgaacggtcc atataaagca gaacgatctt ctcgtccctc atatatgatc
    20941 tttttgacgg aagcaccaaa atcactaagc aatttgctgc agtaactacc agcaaaatca
    21001 gccgacaaat ccaagacctt caaattagaa aatggcgcca tttgattcaa tcaccaaact
    21061 ttttatcacg ctctctggcg cgatcaatcg aggtgactct ttcaccatca taaatctcac
    21121 gcctgtatga cagtcctaag gcgtgattat gaaacccaga cataatgcta ttccgatagc
    21181 ccatctgatc ctgtacttga ttcagggacg ctttggataa tctcaaagca aaggcatctt
    21241 gcaaagctat tctttgagcc agtttcatag tctcttcctc gagttgctca ataggcacaa
    21301 ctttattaac aagtcccatt ttttcggctt cagaagcagt catccaatcc cctgtgaaaa
    21361 ggtattcctt tgcctttcgt actccaattt cgtagggaag acttagatat tggacgtgcg
    21421 ggcctcccca cctcaccgtt ctatcactaa agcgagcatc ctccgaggca acaattagat
    21481 cacaagatga agccaaaatt attcctccca taatacatac cccttgaacc tgagctattg
    21541 ttggtttggg aaaatctctc cacctgaatg tgttcgccac attttgttgc caactccttt
    21601 cgtactctcc tgctgtaccc ggttgataag ggcgtttttc tcgatcagcc atttcttgag
    21661 ggctacccaa gtcatgacct gcagagaaat tagaccctgc ccctgcaatg atcaccacct
    21721 taactgattc atcatctgct gctttatcaa atgcatcatc aagttcttct cgcattatcc
    21781 tactctgagc attcaaaact tcaggtcgat caactgttat cttaaggact gcaccgtcct
    21841 gctttagtcc tagatccgta taagccatta tcacactcca attctagata cttctccgat
    21901 cacaggatgg attatacaat tgaatgtaat gatccaattt gtgtactcat tagtgaaatt
    21961 aaattgatcg caatcaaaac caataaactg acaaaaaaat acgggaactc tctcgcagta
    22021 aacggagttt ctttgacagt caatgtgggt gaagtgtttg cattactggg gcccaatggg
    22081 gcaggtaaaa ctacaactgt agaaatactg gaaggtcaca gaactaaaac atctggagaa
    22141 gtaaaagtgc taggcatgga tccttccaac ggttccaccg aatttcgctc aagggtaggg
    22201 atcgtcttac aggaaactgg aatagaacaa tacctaacag catacgaaat tttggaacaa
    22261 ttcacaaatt tttacaaaac tcctagaaaa atctccgact tactagaaat gactggcctg
    22321 acagaagaaa aagacaagcg agtcaagaaa ctttcaggtg gtcaaaaacg tcgcctggat
    22381 gtagcaatcg gactttgtgg caatcctgat cttttgttcc tcgacgaacc cactacaggt
    22441 tttgaccctt ccgcccggcg cactttttgg gacatgataa aaaaattaaa gactgatggt
    22501 actactgttg tactcacaac tcattacatg gaagaagctg aatatctagc agacaccgtt
    22561 gctcttatga accatggtga attaattacc cagggatcat taagcgatct cagaatttcg
    22621 cacaataaaa catttatcac ttttaaaaca gatgagccac agcttcaact accaaaggac
    22681 ataaaagaaa tagcagtcat gtctgaaggg gctgtttcaa taagcactga aaatccaact
    22741 gccgttcttt cagaattaac aggatgggca atatcacaag gaatcgaact cagtaatcta
    22801 gaggtatcca gacaaaatct tgaagatatt tatttaggaa ttctcaaatg aattttagaa
    22861 agcatttgcc tcttttccta aaacaaataa tctatgaaag taaatcgtac cgaagaaatc
    22921 cggcggctgc tttttttact cttgtttttc ctcttctttt tctatttatg cttagaattt
    22981 ttgggaatga ttcagtggag atagacggtg gcattgtatc cagagccact aaatatatac
    23041 cgatgataac cgccttttca gtaataggct cctcatacac gggaatcgct atgactttga
    23101 ctgtgaacag ggatatagga gtacttaaac gttacaaagg gacccctttg ccattaatcc
    23161 tattcctcct agggaaaata gcgcataaca caatagtgtc ttttgcctta gctgtcatta
    23221 ttgcgataat agcttttgta gtattcgatg ttggtctacc aaccaattca atagtggcat
    23281 ttttactgac gatgattcta ggagcagcta cttttactag tctgggaatt gcctttacga
    23341 gtgtaatccc caattcagat gctgctgcgc caatggtaaa cgcatctgta atacctctga
    23401 tgttcatatc tgatgtattc atcccaatgg atagtgcacc aaattggtta aatggaatag
    23461 cccaagtatt ccctgtaaga ccattttcac tatcattgca ggaaacctac agcccatttt
    23521 catctggcct cactagcaac ccagaaaatt ttgcaatatt attcgcctgg ctggtagtag
    23581 gtgttatcgc ctctttaaaa tttttttcct gggaaccaag aacataatat tttccccaac
    23641 ataccagatg ccctactaaa cgcaccttcc actagagttg tagaggatat aacaaactat
    23701 taccattcat tctaatacta ttcattatta tttcagaatg atggagatgc tatgactgcc
    23761 ttaaaagact taaaagtatt agatttgtcg agaatccttg cggggccgct tggaacccag
    23821 atactggctg accttggagc tgaggtctgg aaagtggaat ccccgtgggg tgacgacacc
    23881 cgtggatggg gccctccttt tgtgggtgat caaagcgcat attacctttc cgctaacagg
    23941 gggaaaaaaa gtattatagt gaacctaaga gatgaaaagg gtcaaaaaat aatcaaggaa
    24001 ctagccaaaa aggctgatat tttcgtagag aattttaagg taggcaattt agctcgattt
    24061 ggcctagatt acgagtccat ttcttcctta aaccctaaaa taatttatgc ttcagttaca
    24121 gggttcgggc aaaccggccc cagagctcca gaaccaggat atgatgcagc cttgcagggg
    24181 atgacggggg taatgagtgt tactggtgaa actgaaggag gccctactaa agtgggtgct
    24241 gcatggatcg atatcatgac gggtctcaca tctactattg gaattttatc agctcttcat
    24301 gaacgttctt tgagtggtaa aggccagcac gttgacgtct cattgtttga tgtcggaatc
    24361 gcgggtatgg ccaatatagc ccaaagctat ttagctagtg gtgaagcacc gggacgaata
    24421 ggaaacgcac atcctcaagt ggctccttac caagatttta cagcctctga cggtgacttt
    24481 atggctgccg ccaataatga cgaccagttt aggcgaatgt gcgaaacagt agaagccccc
    24541 gacttagcag aagatagcag gtttttaact aacgccgacc gcgtgaaaaa tcgaaaagaa
    24601 cttgaaagac tattgaatac ccaattcgtt cagaaatcca aatctcattg gatacctacg
    24661 ctccaagaag caggattaac agtctcctca ataaacacct tggatgaagt ctttaaggac
    24721 ccccaagcca aagctcgcaa atcactatgg caaattgatc atccgactct aggaacaatt
    24781 gatttgttag gaagtgccct tcaacatcta agcagaactc cggcccagcc aacaaatcac
    24841 cctcctctat tgggagaaca tacggaagag gtgatgaaaa atgtcttggg aatggatgca
    24901 caagaaatcc aagaattaat ttcatcagag gttataaagg ggaaataaat aagtccccat
    24961 tccgtaacgc cataaccaag ggaaaagaaa aatttccgct ttaggagaat agtcaacatg
    25021 aaaatcaaaa gtattaaggc aatagcagta aatctaagcc ccaatcccaa aactcagccg
    25081 agagtagagt tagatccaaa tggtggatat tttgtaaacc ctatggatag gtacaaatat
    25141 gattcgaaat acaggtcggg ctcaaattgg aatcgcgtcg catgcgtggt aacagccgaa
    25201 gacggcacat ggggcttggg aacaacaata cactctggtc cagtggtttc cataataaat
    25261 gatcattttt ctgagctcct agtaggtcaa aatccaatgg ccactgaaaa aatatgggac
    25321 atgatgcgta gagcatcttc accatatggg accgcaggcg tagtaagtta cgccattagt
    25381 gctttagaca atgctatatg ggacctcaaa gggaaacttt tagaaacgcc ggtttatgaa
    25441 cttctaggag gaccggtgaa agacgatata ttttgctatg cctccaacac catgttgagg
    25501 tacaaaacca gtgactatat ggattggttc ctggaattag gtttcaaggc cgttaaggta
    25561 tttctgagac acggacctga cgaaggccta gccggattaa acaaaaatgt ggaaataatc
    25621 gcaaatacta gagaacagat tggaccggat attgaattgg cagtcgattc ctggatgtct
    25681 tctaatatcg agtatgcagt gcgtgtgtca gaagcactca aaccatacaa tatcaaatgg
    25741 ctggaggact atatgcttcc agaggatatg gacagttatt ccaaattaag gcaaagagta
    25801 cctagtcaaa ttttagccac gggagagcat tggtacacaa ttcacccatt cgccgatgca
    25861 gcaggtcggg gactagtaga tatattgcaa cccgatgtcc aatgggtagg aggcatcaca
    25921 gcaagtgttc gaatatgcca tatagccgaa gctcatggcc taacagtcat aggtcacgca
    25981 ggaatgaatt atccatatgg tcagcattta gcatattcaa tgcctgtgat tccgtggggc
    26041 gaaaggtcag aaggagtatc agcgcctgga gttccattag aggaaatggt cgcattaccg
    26101 ggaacaccag ttataaagaa tggtcgagta aaaccttctg atgcacctgg gtttggtata
    26161 gaagtggata tcgcctggat agaagagaga gctctatagc aagaagc
//
LOCUS       DASCNS010000047        10244 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000395762, whole genome shotgun sequence.
ACCESSION   DASCNS010000047 DASCNS010000000
VERSION     DASCNS010000047.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000395762"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="QF838_01950"
     CDS             <1..1938
                     /locus_tag="QF838_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59489.1"
                     /translation="REIFSSTRHERSKYMKQLIMLFSISILSVAIFACGPAVGRAVGD
                     TQVDVDATLAEMKPETESEQELYDMLKKIDRLHKEERAELLKALNSSSEVIDELIVLI
                     GDDVDLGNISVSAKNLTDGGADDSDLSDLINRAKSARDREMEILVALENAEKIPWDRI
                     KFLNDQLDAANAELGTLNEDLAEAAKDLDHADQLEAKLVNGLKGQDSEYVALARRAEE
                     ASEKIDVIGHILKVTFKDDEEETATEKLIRMDGRERMADKLEDCMDDSDSENARVARE
                     TCAKEAIAFHQEIKAMEPENVGEIDNAAMLSNPDTEDAVYGVLECLEDLEGMMFTMDM
                     IEDPVTGEFIGRAELYRRNCYRDVNEDILKNGGADSIIERAAISSSDDAATSLKRKLD
                     SDDMATRQIRKDIREGLDDINDKKFKSSVEDLLYNKSLTGQGVEDLMESILDLAQDDL
                     DCDPSGIKGEPCDYVDQYESREYYLEEIAMRLHEEYRALDGIKEDRWASFEEPIRTIV
                     NETAIRLFDNDDMDRFLINERGEVSIAGGDGFNLEIYGIVREISEAVQSNPDLNDGLN
                     NVDADALDDVVNIVASDLIGGLAVGDNWGMAGEVGGKFESCIDSMIENESMEVGDAAA
                     KCLEFTSSAFSAEFNLDNKKN"
     gene            2094..3188
                     /locus_tag="QF838_01955"
     CDS             2094..3188
                     /locus_tag="QF838_01955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HJO59490.1"
                     /translation="MDIVSLADLKQAAKEKIPSDLWDFIEGAAFDEVTKQRNEEKFLD
                     LTMNPNFLIDVGTRNLSTTVFGKKIDFPIMIAPAGGKRQLHPEGEIAAAKGAGMSGTL
                     YALPTASGFSIEEVAEVASGPLWFQLYHSNDEITEFLVTKAKSAGYSAICLTVDGPTS
                     APKEKDLRNNFRRKPELYNGSFRERPEFARSTDIIVPDFSDFSPEEYQGLTWERLDWL
                     KSLTNLPLVIKGIRTVRDALLCAEYGADGIVVSNHGGRQIDRTLSSIETLYPITQAVG
                     ENLEVYFDSGIRRGSDAFMALALGARGVFVGRPAFWGMAYQGAEGVKLMLDILKSEFD
                     RCLAFCGYRNLSEIEIGSVNIPTHWPVNIP"
     gene            complement(3265..4092)
                     /locus_tag="QF838_01960"
     CDS             complement(3265..4092)
                     /locus_tag="QF838_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59491.1"
                     /translation="MEHLLIYPVKKGYLDRDLISEVGKAAEQAGFYAFLSWDHYMLPD
                     GPETLDAWSILGYLAGQTASIKLGTVVTPIPFRPPSQLAKIVASVDHLSGGRTILGVG
                     AGWHQPEFDGFSCWDSPGQRVTRTEEALDLITRLWTGEKVSFEGNNYKSDGAVISPVP
                     TQNPHPPMWFGVRGKRMLQLAAKYGDAWIPTNISPDIYAEGLSYLREKRSKLGINIPL
                     KGALQNFDVFTEAEPCIETINSYGDAGCEYYGSIWSYPPEEMVSRIGWFAEKVMPNV"
     gene            complement(4117..5085)
                     /locus_tag="QF838_01965"
     CDS             complement(4117..5085)
                     /locus_tag="QF838_01965"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HJO59492.1"
                     /translation="MIRTRLTDFLQIQHPILNAPMATSATAELAGAVSESGGFGMLGM
                     GNTIPDPEWLEFQIKRVREITDKPFGVGFITSVDGIDELVSVAVRNKVDLIGHSFSDP
                     SPYIEDAKNAGIKVLAQVQTLGQAINAKEIGVDLIVAQGTDGGGHTGHIGTISIVPAV
                     ADACPEIPVLAAGGIVDGRGVAAALALGASGVWVGSRFVASNEWFGPEWAKQALTEMT
                     TDETIVTKSYDLATDAPFPYEVGHRVIQNKFTKRWHQNYEELLEQKEDLKTDIALAMK
                     NADLSVAPVNASSASGAITSVDKASEIIRNMMDEAEDVLKNISSVL"
     gene            5233..5655
                     /locus_tag="QF838_01970"
     CDS             5233..5655
                     /locus_tag="QF838_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HJO59493.1"
                     /translation="MNYDEALPFLAKNHKGVISTTRPDGSTHSSIVVCGIYNDKPAFV
                     SVYPKSQKILNLRRNPKCTLLSVTGDWRNYVAVEGLISLTDYGNTDKEIMRTQLREAY
                     MSCSDSPHPNWEEYDRAMVRQEAVIVVLEPTRVYGLLR"
     gene            5785..7200
                     /locus_tag="QF838_01975"
     CDS             5785..7200
                     /locus_tag="QF838_01975"
                     /inference="COORDINATES: protein motif:HMM:NF020390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO59494.1"
                     /translation="MVVSDKEEFELRAHLLRRAGFGSTRSELQRQMENSYEDTVEYLL
                     VPNFTDWMGDHIVRRFDGEASGMINAPGASRNWLYRMISTANPLTEKIPLLWHSIFAT
                     GVPKVINGRVLFDQINMLRKYGTGRLDDLLLQLSKDPAMIVWLDNQENHRDAMNENWG
                     RELLELFSMGVGNYTEEDVKECARAFTGWTIGNTEYMMVRAKRDSDWPYGRIAYHFEY
                     REDDHDMEERTFLGQKGNFNGQDIIRIICEQESTARFISRHLYHFFVADEAPVPSWST
                     VAPRDPEAIDQLVQEYFNSDHSITAMLRTLFNSNFFKSQNVRYQKVKGPAEFAATVLR
                     LGGQFNLPNKDMILKYQQIVWMGQELSNPSSVEGWHEGKEWIDTGTLIERINFASDQL
                     SDYENPGVREIVDTLIKDSAGLLSSEETVSNCLAQLGFLEISAETKYILVRYAQKIDD
                     PVSKITGVLRLLTTSREFQLA"
     gene            7208..8203
                     /locus_tag="QF838_01980"
     CDS             7208..8203
                     /locus_tag="QF838_01980"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO59495.1"
                     /translation="MIEYLLTLGIVAMEGRGFEYPTDSVSAPNARLYVTNKSRNYGER
                     GVRITVLDLDSEFYGTFGTFGTSDGKLQSPSAIEATDDGRILVCDDYTHTVNLFDLEG
                     KFLNRWGIYGQGEGELNGPSGLAVDSDNNVYISDTRNHRIQKFNIDGYHIATFGNQGK
                     NNGQFDLPWGISMTPRNTLCIADWGNNRIQELTIDGDVLSVIHNESNPLYELKYPSGA
                     VVDENGLIYISDWGNERVKIINDEGLMIQNLRGEGTISKWAGEFLNTNVEENDARQAS
                     NMDIELDFLDDNDPHSESAHIEKYFWSPVSIKLDKFNRVYVTETNRHRIQVYQQT"
     gene            complement(8209..9396)
                     /locus_tag="QF838_01985"
     CDS             complement(8209..9396)
                     /locus_tag="QF838_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59496.1"
                     /translation="MSNPQMTPRRILIFGSAMHIWSDLIFALLIPLLPAIKDSLGLTY
                     AEVGLLRSTHSGATAVLQVPAGFLAEYFGEFWMLVTGNLWASAGLIGMGVSKGFWLLF
                     VFAIIGGLGGGTQHPLASSMVSRAYNDSGRSAAVGTVNFSGDLGKMVAPALGGTLLIM
                     FGWGGTMFYIGAAGIFLMLVTAAFNRNLNMGRPKPNVTSLSSGDALGESTQNQAFISL
                     SFIGALDNATRTGVLVFLPFILVGHGMGTGEISTALFLLFSGGAAGKYVCGWLDGKIG
                     TVNIIWITKGMTAVFIVLSLYTPYLLIWPLVLVMGVGLNGTSSALYATVAKFVPTSRR
                     GRYYGFFYTTNEIGTIGAPLLYGFVADAFGLVRSTYVMGLVTFAILPLSLRLRKYLSH
                     DVL"
     gene            9479..>10244
                     /locus_tag="QF838_01990"
     CDS             9479..>10244
                     /locus_tag="QF838_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO59497.1"
                     /translation="MKTSLDTSKIGKTDVSVTRIGMGGAPLGGLDSKTARNTLEYAYA
                     QGIRYFDTAPLYGSGLSEIHNGEFLSTLKRTDFVLSSKVGRLIIPGQDIPFNYTKEGI
                     LRSIDESLTRLNLDSIDIVLIHDPDDHYSSALNEAFPTLAKLREEGVIKAIGAGMNQW
                     EMLRRFAADADFDCFLVAGRYTLLDHSALHELMPLCLEKDISLILGGPYNSGILASDL
                     DTKSTYFYDPSPLEVIERARKIKRVCDRHRIPLKAAA"
BASE COUNT         3209 a         2141 c         2251 g         2643 t
ORIGIN      
        1 agggagatat ttagttccac cagacacgag aggtcaaaat atatgaagca gttgattatg
       61 cttttttcca tttcaatatt atccgtagct atatttgcct gcgggcctgc tgtaggtaga
      121 gccgtgggtg atactcaagt agacgtggat gccacacttg ctgaaatgaa gccagaaaca
      181 gaatcagaac aagagctcta tgacatgctt aaaaagattg atcgcctgca caaggaagag
      241 cgagcagagc tgttaaaagc tcttaacagc tcaagcgaag tgatcgatga acttattgtc
      301 ttgataggcg atgacgtaga cctcggtaat atatcagtgt ctgcaaaaaa tttaaccgac
      361 ggtggagcgg atgattcaga tctttcggat cttataaacc gggccaaatc agccagggat
      421 agggagatgg agattttagt agcacttgaa aatgctgaga aaattccatg ggacaggatt
      481 aaatttttaa atgaccagct agatgccgca aacgccgaac tgggaactct aaatgaggat
      541 cttgccgagg cagctaagga tctagatcac gccgaccaac tggaggcaaa attggtgaat
      601 ggtttaaaag gtcaggatag tgaatacgtt gccctagcaa ggagggcaga agaagcctca
      661 gagaaaatag atgttatcgg tcatatatta aaagtgactt tcaaagatga tgaagaggag
      721 acagccacag aaaaacttat ccgaatggat ggccgagaaa gaatggcaga taagttggaa
      781 gattgtatgg atgactctga ttcagaaaat gctagagtag ccagagaaac ctgcgcaaaa
      841 gaggctattg catttcatca agaaatcaaa gcaatggaac ctgaaaatgt gggtgaaata
      901 gataacgctg ccatgttatc caacccggac actgaagatg ccgtttatgg ggtactagag
      961 tgtttggaag atctggaagg tatgatgttc acaatggaca tgatagaaga tccagtaaca
     1021 ggtgaattca taggtagagc tgagctttac agacgaaatt gctatagaga cgtaaatgaa
     1081 gatattctaa aaaatggtgg ggcagattcc attattgaaa gggcagcgat tagtagtagc
     1141 gatgatgcgg ccacatcact gaagagaaaa ctcgattctg acgacatggc cactagacag
     1201 attaggaagg acataagaga aggtcttgat gacataaacg acaagaaatt caagtccagt
     1261 gtcgaggacc tactttacaa taaatctttg acaggccaag gtgtggaaga ccttatggaa
     1321 agcattcttg acctcgcaca agatgaccta gattgcgacc ctagtggtat caagggagaa
     1381 ccttgcgatt atgttgatca gtatgagtct cgcgaatatt acctggaaga aatagctatg
     1441 agattacatg aagaatatcg tgcccttgac ggtattaagg aagatcgttg ggcatctttc
     1501 gaagaaccaa tccgaactat tgtcaacgaa accgcaatac ggctatttga caatgatgac
     1561 atggatagat ttttgatcaa tgaaagaggc gaagtttcca tagctggcgg cgatggtttt
     1621 aaccttgaga tatatggcat agttcgggag atttcagagg cagtccagtc aaatcccgat
     1681 ttgaatgatg gcttaaataa cgtagatgcc gatgcgcttg atgatgtggt taatattgtt
     1741 gcatcagacc tcattggtgg actcgcagtt ggtgacaact ggggtatggc gggagaagtt
     1801 ggtggcaaat ttgaatcgtg tatagactct atgattgaga acgaaagtat ggaagtcggt
     1861 gatgctgcag caaaatgctt agagtttaca tctagcgctt tttctgctga attcaacctg
     1921 gacaataaga agaactaacc atctctcaca atttatttga ttgaataaag aaacaccggt
     1981 ccaaattatt taagccagtg cttctttatt ggtatcatac cgagattgac ttcattactt
     2041 ccttttagcg aaaattggtc aattctactt ggcaattttt catggagatc taaatggata
     2101 tcgttagcct cgccgaccta aaacaagcag cgaaagaaaa aataccgtca gatctatggg
     2161 attttataga aggtgctgct tttgatgagg tcaccaaaca gaggaatgaa gaaaaatttc
     2221 ttgaccttac catgaatccg aatttcttaa tagatgttgg aactaggaac ctttctacca
     2281 cagtttttgg gaagaaaata gactttccta taatgatcgc tcctgcaggt ggtaaaagac
     2341 agttacaccc tgaaggggag atagccgccg ccaaaggggc cggaatgtcc ggaaccctat
     2401 acgctcttcc cactgcatct ggattcagta tcgaagaagt agcagaagtg gcatcgggac
     2461 ctctttggtt ccagctgtat cactcaaatg atgaaataac agaatttctc gtgacgaagg
     2521 ccaaaagcgc tggatactct gccatttgcc tgaccgtcga tggtccaaca tccgctccta
     2581 aagaaaagga tttacggaac aattttagac gtaaaccaga gctatacaac ggtagtttca
     2641 gggaaaggcc ggaatttgcg agaagcacag atatcatcgt tccagatttt tcagatttta
     2701 gtccggaaga ataccaaggg ttaacctggg aacgattaga ttggcttaaa tcactcacca
     2761 acctaccctt agtaataaag ggaataagga cagttagaga tgctttactc tgcgccgaat
     2821 acggtgccga tggaatagta gtttcaaatc atggaggacg gcaaattgac cggacattat
     2881 cttccataga aaccttatat ccaataaccc aagcggttgg ggaaaactta gaagtatatt
     2941 ttgattctgg gataagacgt gggtcagatg cttttatggc cttagcttta ggagctagag
     3001 gggtatttgt aggtagaccc gcattttggg ggatggcata tcagggcgct gaaggagtca
     3061 aattaatgct tgatatcctc aagtcagaat tcgacagatg tttggctttt tgtggctaca
     3121 gaaacttatc tgagatagaa atcggctccg taaacatacc tactcactgg ccagtgaaca
     3181 taccctagca aaattgagga ggtatccata tacaaatcaa cttgacccat ggggacctca
     3241 gctaatcttt ttcagaaaca atgatcacac attgggcatt actttctcag caaaccatcc
     3301 aattctactg accatctctt caggaggata agaccaaata gaaccataat attcacaccc
     3361 agcgtctccg tatgaattaa tagtttctat acagggctca gcttctgtga aaacatcaaa
     3421 gttttgcaac gctcctttca aaggaatatt tataccgagt tttgaacgct tctctctaag
     3481 ataactaagg ccttccgcgt atatatcagg gctaatattc gttgggatcc aagcatctcc
     3541 gtattttgct gctagttgaa gcattctttt gccccttaca ccaaaccaca taggcgggtg
     3601 tgggttttgc gttggaaccg gggaaattac cgcaccatcc gacttgtaat tattaccttc
     3661 gaaactcacc ttttcacctg tccacagtct tgtaatcaga tctagcgcct cttctgtcct
     3721 tgtcaccctt tggccaggtg aatcccaaca actaaagccg tcaaattcag gctggtgcca
     3781 acctgctcct accccaagta tggttctacc tccagacaag tggtcaacac tagccacgat
     3841 tttcgctaac tgagatgggg ggcgaaaggg aattggtgta actaccgtac caagtttaat
     3901 agacgcagtc tggccagcaa ggtaaccaag tatgctccag gcatctaagg tttccggccc
     3961 atcgggtagc atgtaatggt cccacgacaa gaatgcataa aaccctgctt gttccgccgc
     4021 tttaccgact tcagaaatta ggtctctatc caaatatccc tttttgaccg ggtaaatcaa
     4081 caagtgttcc aactgatgct cctaataaaa tcattgttat agtactgaac ttatattttt
     4141 cagcacatct tctgcttcat ccatcatatt ccgaataatt tcggatgctt tatccacaga
     4201 ggtaattgcc ccagaggcac tactcgcatt taccggagcg acagataaat cggcgttttt
     4261 cattgccagg gctatatctg tctttaaatc ttctttctgt tccagcaatt cttcatagtt
     4321 ttggtgccat cttttagtaa atttattttg gatgactcta tggcctactt cataaggaaa
     4381 gggggcatca gtggccaaat cataggattt agtaacaata gtttcgtccg tggtcatctc
     4441 tgttaaggcc tgtttggccc actcggggcc aaaccattcg ttcgaggcaa cgaatctact
     4501 gccaacccaa accccacttg ctcccaaggc caaagcggca gcgacacccc ttccgtccac
     4561 aataccacca gccgccaaca caggaatttc cggacacgca tcagctaccg caggaacgat
     4621 cgaaattgta cctatatggc cggtatgccc gccaccatcc gtaccttggg ccactatcaa
     4681 atcaacccct atttccttgg cgtttattgc ctgtcccagt gtttgcactt gagccaaaac
     4741 ttttataccc gcatttttag catcctctat gtatggagat ggatctgaaa aggaatggcc
     4801 aatcagatct accttattcc ttactgcgac agaaactagt tcatctatac catcgacact
     4861 agttataaaa cccacaccaa atggcttatc tgtaatttcc cgtacacgtt taatttgaaa
     4921 ttccaaccac tcaggatctg gaatggtgtt gcccattccc agcatcccaa atccacctga
     4981 ctcagatacg gctcctgcca attctgcagt agcactagta gccatcggtg catttaaaat
     5041 cgggtgttgt atctgaagga aatcggtcaa tcttgttcta atcatagaat tagttccata
     5101 atttcattct taaatatatt tccactttac tatatatacc acacagaagc tgtaaatcct
     5161 gtatacacca tcagaaataa catagttgga tagcagcaaa taagttaatt actcggttaa
     5221 tggaggatca aaatgaatta cgatgaagct ctccctttcc tagctaaaaa ccacaaaggt
     5281 gttataagta caacaaggcc ggatggatcc acacattcca gcatcgtagt ttgtgggatt
     5341 tataatgaca aaccagcttt cgtatctgta tatcccaaat cacaaaaaat tttgaactta
     5401 agaagaaatc ccaaatgcac tctcctatca gtaactgggg attggcgaaa ttatgtggca
     5461 gtggaaggat taatttctct cactgattac ggcaatactg ataaagaaat aatgcggacc
     5521 caacttaggg aagcatacat gtcttgcagt gacagcccac atccaaactg ggaagaatac
     5581 gatagagcaa tggtacgaca agaggctgta atagttgttc tggaaccaac acgagtttac
     5641 ggcttgttac gttagataga atactgtgat ataaatactt aagtttttcc aacacctgtt
     5701 ggccccggaa atatgaaggc cccagttggc cttctagggt tttcaagaaa gagcgacaat
     5761 caataaagtc gacagagaaa aaccatggta gtttctgaca aggaagaatt tgagttaaga
     5821 gcccatctac tcagaagagc agggttcgga tcaactaggt cggaacttca gagacaaatg
     5881 gaaaattcct acgaagatac tgttgaatat cttctggtcc caaattttac cgattggatg
     5941 ggagaccata ttgtaagaag attcgatgga gaggcatcag gaatgataaa tgcccctggg
     6001 gcatcccgta attggctata cagaatgatc agcacagcca atccattgac agaaaaaatc
     6061 ccccttctct ggcacagtat ttttgccact ggcgtgccaa aggtcattaa tggaagagtg
     6121 ttatttgatc agataaatat gctccgaaaa tatggtaccg gaaggttgga cgacctattg
     6181 ttacaacttt caaaagaccc cgcaatgatc gtatggttag ataatcaaga gaaccatagg
     6241 gatgcgatga atgaaaattg gggtagagag cttttggaac tgttttccat gggtgttgga
     6301 aattacactg aagaagacgt aaaggaatgt gctagagcat tcacgggctg gactatcgga
     6361 aacactgagt acatgatggt ccgagcaaaa agagactccg attggcctta tggaagaatc
     6421 gcctaccatt tcgaatatcg agaagacgat cacgatatgg aagaacgaac ctttctaggg
     6481 caaaagggaa atttcaacgg tcaggacatt atccgaataa tttgcgaaca agaaagtaca
     6541 gcaagattta tatcaagaca cctttatcac ttttttgtgg cggatgaagc accagtccct
     6601 tcatggtcta cagtagcacc tagagaccca gaggcaattg atcaactagt tcaagagtac
     6661 ttcaactcgg atcacagcat aactgcgatg ctgcgaaccc tcttcaattc aaattttttc
     6721 aaatcccaaa atgtcaggta ccaaaaggtg aaaggacctg ctgaatttgc ggcaactgtt
     6781 ttgagacttg gtgggcaatt caatcttcca aataaagata tgatcctgaa atatcaacag
     6841 attgtttgga tgggtcaaga gttaagcaat ccctcgagcg tggaaggttg gcatgagggg
     6901 aaagaatgga ttgatactgg gacactgata gagagaatta attttgcatc agatcaactg
     6961 agcgattacg aaaatccagg tgttcgcgaa atagtagaca cattgataaa agacagtgct
     7021 ggactcctgt ccagtgagga gacagtctca aattgtcttg cacaactagg cttcctggaa
     7081 atctctgctg agactaaata tattttagtg agatatgctc aaaagattga cgatccagtt
     7141 tccaaaataa ctggtgtact tcgtcttttg accacttcca gagaatttca attggcgtag
     7201 ccggaaaatg attgaatatt tgcttacgct aggaattgtc gcaatggaag gaaggggatt
     7261 tgagtaccca acagactcag tgtcagctcc gaatgcccgt ttgtacgtaa caaataagtc
     7321 gaggaattat ggagaacgag gggttagaat aactgtatta gacctcgaca gtgaatttta
     7381 tgggacattt ggaacttttg gaacctcaga tgggaagctg cagtcccctt cggctattga
     7441 ggctacggac gacgggagaa tccttgtctg cgatgactat actcatactg ttaatttatt
     7501 tgacttagaa ggtaaatttt taaaccgatg ggggatttac ggtcaaggtg aaggtgaact
     7561 taacggtcca tcgggattag ctgttgattc tgataataac gtgtacatat cagatacccg
     7621 caatcacagg atccaaaaat ttaacatcga tggttaccat atagccactt tcggtaacca
     7681 aggaaaaaat aacggtcaat ttgatctacc atggggtata tcaatgactc ctcgaaacac
     7741 attgtgcatc gccgactggg gaaataaccg catacaagaa ttaacgatag acggggatgt
     7801 tttatcggtc atccataacg agtcaaatcc tttatatgaa ttgaaatacc cttctggcgc
     7861 ggtggtggat gagaatgggt taatttacat atcggactgg ggaaatgaaa gggtgaaaat
     7921 aataaatgat gagggcctca tgatccaaaa tttaagaggc gagggaacaa tttcaaaatg
     7981 ggctggtgaa tttctaaaca ctaatgtcga agaaaatgac gcccggcaag catcaaacat
     8041 ggatatagaa ttggatttcc ttgatgataa tgatccacac agcgagtccg ctcatataga
     8101 aaaatatttt tggtccccag tttcaatcaa attggataag tttaaccggg tatacgtaac
     8161 cgaaactaat cgccaccgaa tacaggtata tcaacaaacg taggaaaatt acaagacatc
     8221 atgtgacaag tatttcctca accgaagact taacggaagt attgcaaaag taaccaagcc
     8281 cattacataa gtagaccgca ccaagccaaa agcatccgcc acaaacccgt acaataaagg
     8341 cgccccaatc gtacctattt cgttggtagt ataaaaaaat ccataatacc ttcccctccg
     8401 actggtaggt acaaacttag ctacagttgc atagagggcg ctagaggtac catttaaccc
     8461 aacgcccatc acaagtacaa gaggccaaat aagcagatac ggagtgtaaa gtgataagac
     8521 gataaaaacc gccgtcattc ctttagtgat ccaaataatg ttaaccgtgc caattttgcc
     8581 atccaaccat ccacatacat atttacccgc ggctccaccg gaaaacaaaa gaaataaggc
     8641 agtacttatt tcacccgtcc ccattccgtg accaactaga ataaaaggta ggaaaacgag
     8701 tacgcctgtc cgagtagcat tgtctagtgc ccctatgaaa ctcaatgaaa tgaacgcctg
     8761 gttctgagtt gattctccta aagcatcacc tgaggaaagg ctggtcacat tcggtttagg
     8821 tcgccccata ttaaggttac ggttaaaagc tgcggtcacc aacattaaga aaattcctgc
     8881 agcccctatg tagaacatgg ttccacccca accgaacata attagcaagg ttccgcccaa
     8941 tgctggagca accatttttc ccaaatcccc tgagaaattt acggttccta ccgctgctga
     9001 gcggccagaa tcattatatg cccgagaaac catactggaa gccaatggat gctgggttcc
     9061 ccctccaaga cctccaataa ttgcgaacac aaatagtaac caaaacccct tcgatacccc
     9121 catccctatt aaccctgcag aggcccataa gttacccgtt acaagcatcc aaaattctcc
     9181 gaaatactct gccaaaaaac ctgctggtac ctgcaataca gcggtagccc cactatgagt
     9241 agaccggagt aacccaacct cggcgtaagt caaacctaaa ctgtctttaa tagcaggcag
     9301 gagcggtatc agcagtgcaa atatcagatc gctccatata tgcatagcag atccaaagat
     9361 caaaatacgc cggggagtca tttgtgggtt ggacatgtat agaaatatct cgtggttagg
     9421 taaaatgatt tgtgatgtta tcagggctca cataataaat cacattcagg tgtagcatat
     9481 gaaaactagt ttggatactt caaagatagg gaaaaccgac gttagtgtta ctagaatagg
     9541 tatgggtgga gcccctttag gtggactaga ctctaaaacc gcccgaaaca ccctggaata
     9601 tgcctatgca caaggcatac ggtattttga cacggcacct ttgtatggat cagggctgag
     9661 tgaaatacat aatggggaat ttctttcaac attaaaaaga accgactttg tcctatccag
     9721 taaggtaggt cggttgataa ttcccggcca agatatcccg tttaattaca caaaagaggg
     9781 gattcttagg tccatagatg aaagtctcac aagattgaat ttggattcaa tagatatagt
     9841 gctgatacac gatccagacg accattatag ttctgcgttg aatgaagcat tccccactct
     9901 ggcaaaactt cgagaggaag gagtcataaa agctataggg gcgggtatga atcaatggga
     9961 gatgctgagg cggttcgccg cagatgcaga ttttgattgt tttcttgtgg caggcagata
    10021 cacattgctt gaccactcgg cccttcatga attaatgccc ttgtgtttgg aaaaagatat
    10081 aagcctgata ctcgggggtc cttacaatag cggaatacta gcatctgacc tggatactaa
    10141 aagcacctat ttctatgacc cttcacccct tgaagtaata gaaagggcaa gaaagataaa
    10201 acgggtttgc gatcgtcatc ggatacctct caaagcagct gcac
//
LOCUS       DASCNS010000048         2651 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000399071, whole genome shotgun sequence.
ACCESSION   DASCNS010000048 DASCNS010000000
VERSION     DASCNS010000048.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000399071"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            50..125
                     /locus_tag="QF838_01995"
     tRNA            50..125
                     /locus_tag="QF838_01995"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83..85,aa:Trp,seq:cca)
     gene            139..1827
                     /locus_tag="QF838_02000"
     CDS             139..1827
                     /locus_tag="QF838_02000"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HJO59498.1"
                     /translation="MRLDNPQTTKENRVIGADLVAQILKAEGVDFMGVIPFNRLEEAG
                     AQAGIRPLIFRQERVGVNLADGYSRITNGDKTGVFAMQAGPGAENAFAGVATAYADSV
                     PILLLPASNPRARHGVHPTFSPSETYRTVTKWSGKVAMPESIPDMMRRTFSQLRLGRP
                     GPVLLEIPSDILSDEIPEGMNTSYTNVENRKSAGDPQDIKTAAKMLLGSSRPVIQAGQ
                     GVLYGKASKQLLELAELTRVPVMSSMAGKSSFPETHELALGTRSSSTTDMVVHFIDKA
                     DMIFGIGTSFTRIHYGMNEFDKKTLVQITNSSDDINKDYSPDHAVLGDVQLVLEELIV
                     EVKALLGSSARNESYKFEDEIASVKEDWFKEWMPHLKSDETPISPYRVIWELMQNTNP
                     AETIVTHDSGSPRDQIIPFYNSVNPNGFIAWGKSTQLGYSLGLAMGASMAEPNKVAVN
                     LIGDYGFGMVGLDIETAVREKIPVLTIILNNSAMGIYRPHNFPTANELYGTKYTTGDY
                     AKIADALGSYNEVVDNPNEVGAAILRCVDTVKGGNTAVLEVMTKEEPAIPHRMF"
     gene            complement(1831..>2651)
                     /locus_tag="QF838_02005"
     CDS             complement(1831..>2651)
                     /locus_tag="QF838_02005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HJO59499.1"
                     /translation="MNLPLGPVHSRTNPPESFNKTIHFLNPDFAMLMGRTGLRKGCES
                     YFFLTYDRCLTWQGPYSFPMFGELGIAARTDIIKDKSDTHRAKLFLTSTKLDGDEGRV
                     FCAETQNGGRSFNFISWVRESPEGFDIMPSSLKLPSGRLLTALRCRSSDNNRGWIDLM
                     YSDDEAISWAQLSKPVANTGVGGNPPAMLRLSDSRICLVYGYRDEGSGIRYVVSEDEG
                     ETWGKELIIREDAGSHDIGYPRVVERSDGNLVAVYYHNDAADGERHIVATIWKP"
BASE COUNT          797 a          599 c          608 g          647 t
ORIGIN      
        1 tgtcaatatg atttttgata tagttttata ctctagattc actaaactca ggggtgtagc
       61 tcagttggta gagcgaaggt ctccaaaacc ttaggtcgtg ggttcgatcc ctgccacccc
      121 tgccagtgac cactagaaat ccgcttagat aatcctcaaa caacaaagga aaatagagtg
      181 ataggcgcag atttagttgc ccaaatactt aaagcagaag gtgtcgactt catgggagta
      241 atcccattta ataggttgga agaagctggg gcgcaggcag gcataagacc gctcatattt
      301 agacaggaac gggtcggagt taatcttgcc gacggataca gccgaataac gaacggcgac
      361 aaaactggtg tgttcgcaat gcaagccggg ccaggggcgg aaaatgcctt tgccggagtt
      421 gcaactgcat atgcggattc ggtacctatt ttactgcttc ctgcatcaaa tcctagggct
      481 aggcacgggg ttcacccgac attcagccca agcgaaacat accgaacagt aaccaaatgg
      541 tctggcaaag ttgcaatgcc tgaaagtatt ccagacatga tgaggcgaac attcagtcaa
      601 ctcaggttag gtcggcccgg gcctgtgctt ttagaaatcc ccagtgatat tctaagcgac
      661 gaaatacctg aagggatgaa caccagctat acaaatgtag aaaacaggaa aagcgcagga
      721 gaccctcagg atataaaaac cgcagctaaa atgttgcttg gttctagtag acctgttatc
      781 caggcgggtc aaggggtttt atatgggaaa gcatccaaac aattattaga actcgccgaa
      841 ctaacccggg tcccagtaat gtcatcaatg gcgggcaaaa gctccttccc tgaaacacac
      901 gagttggccc tcggaacccg cagtagctcc acaacggata tggttgttca ttttatcgat
      961 aaggcagaca tgattttcgg cataggtacg agtttcacac gaatccatta tggaatgaat
     1021 gaattcgata aaaaaacact cgttcagatc acaaacagct ctgatgacat aaacaaagat
     1081 tactcaccag accacgcagt tcttggcgat gttcaactag tattagaaga attgatcgtc
     1141 gaggtaaagg cattattagg ttctagcgcc cgaaatgaat catacaaatt tgaagatgaa
     1201 atcgcctcgg taaaagaaga ctggtttaag gaatggatgc ctcacctaaa gtcggatgaa
     1261 actcccatta gtccttatag agttatctgg gaattgatgc aaaacaccaa ccctgcggaa
     1321 actatagtaa ctcacgattc tggtagtcct agagaccaaa taattccctt ttacaattct
     1381 gtgaatccca atgggtttat agcatggggc aaatcgacgc agttggggta cagtcttgga
     1441 ttggccatgg gtgccagtat ggcagagccc aacaaagtgg ctgtcaactt gatcggagac
     1501 tatggtttcg gaatggttgg gctcgatatt gagacagcag tcagggaaaa aatcccagta
     1561 ttaaccataa tcctcaataa ttcagcaatg gggatataca ggccgcataa tttccctacg
     1621 gcaaatgagc tatatggaac taaatacaca acaggtgatt atgccaaaat agctgatgcc
     1681 cttggcagct ataacgaagt agtagataac cctaacgaag tcggagcagc tatcttaaga
     1741 tgcgtagaca ctgttaaagg tggtaatacc gctgtactgg aggttatgac aaaagaagaa
     1801 cctgcaatac cgcacaggat gttttaggca ctaaggcttc catatcgttg ccacgatgtg
     1861 tcgttctccg tccgcagcat cattgtggta atagaccgca actaggtttc catcggatct
     1921 ttccactact cttggatacc cgatgtcgtg gctgcctgcg tcttcccgaa taatgagctc
     1981 ttttccccag gtttcacctt catcttcgct caccacatat ctgatccctg aaccttcatc
     2041 ccggtaacca taaaccaaac agattctgct gtccgaaaga cgaagcatgg ccggaggatt
     2101 accgccaaca cccgtattgg ctacaggttt tgacaattgg gcccacgaaa tagcctcatc
     2161 atctgaatac attaaatcga tccaacctcg attattatca gaggagcggc aacgaagagc
     2221 agtaagtaat ctaccagagg gtaatttcaa ggaagaaggc ataatatcaa agccctccgg
     2281 cgattccctt acccaggaaa taaaattaaa tgaccggccc ccattttgcg tttcagcaca
     2341 aaataccctg ccctcatctc catcaagttt ggtggaagtc agaaataatt tggccctatg
     2401 ggtatctgat ttatccttaa taatatctgt acgggccgct atcccgagct ctccaaacat
     2461 tggaaaggag tagggaccct gccaagtaag acaccgatca tatgtcagaa agaaatatga
     2521 ctcgcaccct tttctcaaac cagttctgcc cataagcatg gcgaaatctg ggtttaaaaa
     2581 atggattgtt ttattgaatg attctggtgg gttcgttcgg gaatgcacgg gaccgagtgg
     2641 gaggttcatg t
//
LOCUS       DASCNS010000049         6224 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000411211, whole genome shotgun sequence.
ACCESSION   DASCNS010000049 DASCNS010000000
VERSION     DASCNS010000049.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000411211"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(24..449)
                     /locus_tag="QF838_02010"
     CDS             complement(24..449)
                     /locus_tag="QF838_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59500.1"
                     /translation="MFIGHFTERPYQDPKSGYFGATGKSIKDLTLSNDIYDPMLGSDL
                     YNRYLDEKIYAEEMGFDGLMLNEHHSSPFCMGSTVNVEASILARITNKAKIVLLGNVL
                     PIWDDPLWLVEQLATIDMISKGRLVFHRGFWRGLYQKLS"
     gene            complement(477..1277)
                     /locus_tag="QF838_02015"
     CDS             complement(477..1277)
                     /locus_tag="QF838_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59501.1"
                     /translation="MAGEISQFEEQEIMIAEGKLQMRIAGEGEPLLLLHGELGFPGWM
                     KYHRALSEQFRVMAPSHPGYDGSDRLDWAMHVSDLAGVYLQVLDDLDLSGIKMVGFSM
                     GGWLAAEMAAMDPGKFSKLVLVGPMGVKPEEGEIYDLFLNLATEFLEESFLDPNSVEE
                     YATLRPDEPSPEQEELWEVGREQSARLAWKPFMYNPTLPYLLRRLKTLQTLIVSGRQD
                     SIVPIDSLRIYNESIPGSSLSLIDQSGHRPEIEKPEDFLKLLINFLKK"
     gene            1445..2569
                     /locus_tag="QF838_02020"
     CDS             1445..2569
                     /locus_tag="QF838_02020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59502.1"
                     /translation="MPNIKSGIFIMPFHPPGKALAQCHDEDMELLIESDKLGIDEFWV
                     GEHHTLSWEPIVSPEMFIAAGFRQTKRIRIGPAPLLLNFHHPAQVANRLSFLDHFSHG
                     RLNIAFGHGGAPSDFELYNLQSSEMQKMSEEAMGMILQMWEKEPPYEITGNYWNITLK
                     SSDEDIGYGKIQKPLQTPYPPISVPITGYNSNSAKVAAKYGFSPFSHSITSLPVLKDN
                     WKTYSSASRLGNTEPDRSNWKLARTIFIADSDKEAIKKARNNSISKAYKYLATAIKKG
                     PGLAIMKSDPNDPDSEITEDYLINELVIAGSVETVISRLQEVTEYLGPFGTLINMSFD
                     WIDESDKRDWLNSMALFQNEVIPKLSSQINQLIAPENSND"
     gene            2562..3374
                     /locus_tag="QF838_02025"
     CDS             2562..3374
                     /locus_tag="QF838_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO59503.1"
                     /translation="MTNNYRQNRTKSNLLKGKTIKVPEINRVFDPTVVETIASVGYEC
                     VWIDMEHSHADFKDLATLVLAARASEIDVFVRIPHGPYNQIIKTLEIGASGLIWPHCK
                     SKKEAEAFVEMAKYHPEGMRGMGGGRDSSFGKSEKPAYYDIANENVLLGVMIEDKEGV
                     EACGEIASVPGIDLLFVGPGDLSQSYGVHRDPGNPISQPIILKAFDKVGSACKLHGKF
                     MGTAVDPGEPMAMAVARGATWLNCCHDVTALTTGYESAMVKTDQIVASTNQI"
     gene            complement(3371..4429)
                     /locus_tag="QF838_02030"
     CDS             complement(3371..4429)
                     /locus_tag="QF838_02030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59504.1"
                     /translation="MGYDEAWIGEHHSAGWETISSPELFIAAVAERTRRISLGTGVVS
                     LPYHHPFMVANRMVQLDHMTRGRVMFGVGPGALPTDAYMMGIDPTTQREKMDEALGVI
                     LRLFTEIEPITYKSDWFELNEALLQLRPYQSPHMPIAVASIQSPSGVQLAGKHGASVL
                     TITVPRDPLADRADYGKLWDIAEESAEQHGKVVKREDWRLVVPVHLAETREKAREQAR
                     EGAAGFLRDYSEGTNGRKPVFDGPRDQVVDWMSDNGFWIVGTPDDCIEGINNLARESD
                     GFGGFLVQTIDWAPSDSILRSYELLARYVMPQFQGSLTGLEVSNQWAKDRVDVLLDGR
                     IRAIEKAKSDYRGSVNLS"
     gene            complement(4435..4563)
                     /locus_tag="QF838_02035"
     CDS             complement(4435..4563)
                     /locus_tag="QF838_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59505.1"
                     /translation="MNLPEKMKFCIFLAPFHKVGENPTLALERDMELIQWLDKLGG"
     gene            4708..5988
                     /locus_tag="QF838_02040"
     CDS             4708..5988
                     /locus_tag="QF838_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59506.1"
                     /translation="MKPKFFYGWWIALTALVVNAVISAPAFGATGLWIDSLETEFGWS
                     RTQLSIAFSLGQLEGSVAAPFVGYLIDKIGGKKVACIGAIASSVGFLCLSQTVPITES
                     RENWLDPAIFYISYVTIMFGVTLGGWIPMTVIINNWFAKHRSLAMSIGSVGFSIGTFA
                     IVPFLAILIESESVGWKTTALGLTLFFPLLIFPITRIIKNTPEEIGEVPDGIEATSTV
                     NSIPDQSVEIGSNDFSLKAALQEKVFWYIAIGHGASAMLTSTMMVHLILAFKHQGISL
                     QTAAFIWGLAMGVGGVSQILGGFLGDRTKKHVAVCIFGCIQAIGVSLAVLVTNTGTAL
                     IFAIVYGIGFGARAPITTSMRGDYFGRKAFGKIMGVSAMSMMLMTMFGPIFAGRLFDL
                     QGNYQNGFLILACCGFIGSLIFLLAKRPVLQKTG"
     gene            complement(6041..>6224)
                     /locus_tag="QF838_02045"
     CDS             complement(6041..>6224)
                     /locus_tag="QF838_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HJO59507.1"
                     /translation="SKVVQQYFTGQIFEYPVVEGIKTHMLLTPLADLHKPKLTIEDLS
                     DLEGTQVIFRDLGILD"
BASE COUNT         1779 a         1428 c         1265 g         1752 t
ORIGIN      
        1 attttaattc atttcatgac gatttaagat aacttttgat acaggcctcg ccagaatcct
       61 cgatgaaaaa ccaaccgacc cttggaaatc atgtcgatgg ttgctagttg ttcaaccaac
      121 caaagtggat catcccatat aggaagaaca ttacccaaaa gtactatctt tgccttattc
      181 gtaatacggg cgagaatgga agcttctacg ttaacggtac tacccatgca gaaagggctg
      241 ctatggtgtt cgtttaacat caagccatca aatcccattt cctccgcata aattttttca
      301 tctaagtacc tgttatacag gtcggatcct aacattggat cgtatatatc attgctaagg
      361 gtcaggtcct tgatagactt gcccgtcgcc ccaaagtagc cggacttggg gtcttgatat
      421 ggcctctccg taaaatgtcc tatgaacata tattcctccc tatgtgggca aatttatcat
      481 ttttttagga aatttattaa taatttaagg aagtcctcag gtttttctat ctccggccta
      541 tgaccactct ggtctattag acttagtgag gatcctggta tagattcgtt atatatcctc
      601 aatgaatcaa taggaactat agaatcttgt cttccagaca cgatcagtgt ttgaagtgtt
      661 ttcaacctcc gcagtaagta aggcaaagtc gggttataca taaaaggttt ccatgctaat
      721 cttgcagact gttcgcgtcc tacttcccac aattcttcct gttccgggga aggctcatcg
      781 ggtcttaaag ttgcatactc ttccactgaa tttgggtcca aaaaactttc ttccaaaaat
      841 tcggtggcta gatttaggaa aagatcgtaa atctcaccct cctctggttt aacacccata
      901 ggtccgacaa gtacaagttt tgaaaactta ccaggatcca tcgcagccat ttccgcagcc
      961 agccaacctc ccatcgaaaa acccaccatc ttgattcccg ataaatcaag gtcatccagc
     1021 acctgaagat atactccggc taaatcactt acatgcatag cccagtccag tctatctgac
     1081 ccgtcatacc ctggatgact tggtgccata actctaaatt gttctgacag tgctcggtga
     1141 tacttcatcc aaccgggaaa acccaactca ccgtggagca acagcaacgg ttctccttcc
     1201 cccgcaatcc gcatctgaag ttttccttcc gcaatcatta tttcttgttc ttcaaattgg
     1261 gagatttcgc ctgccattcc ttattcctct tatggtttcc atcaatcagc acaaagccta
     1321 attacgcgat tatagctgaa aatatgggaa agccggtgtt ttaagaattt gttcgattag
     1381 tttggttgca tttatactgc cgccatgatg aactttagct tatccaatct ggtggggttc
     1441 taacatgcct aacataaaat ctggaatctt tataatgccg ttccatcctc cgggcaaagc
     1501 attggctcaa tgtcacgatg aggacatgga gctgctaatt gagtcggata aactaggaat
     1561 cgatgagttt tgggttggcg agcatcacac tttgtcatgg gaaccgatcg tcagtcctga
     1621 aatgttcata gcggccggat tcaggcaaac taaacgaata agaattggac ctgcacccct
     1681 ccttttaaat tttcatcatc ctgcccaagt cgcaaacaga ctttcgtttt tggatcattt
     1741 ttcccatggc cggcttaaca tcgcctttgg ccatggtgga gcaccgtcag attttgaact
     1801 atacaatctc caaagttcag aaatgcagaa aatgtcagaa gaagcgatgg gcatgatatt
     1861 gcagatgtgg gaaaaagaac ctccctatga aatcaccggt aattattgga acataactct
     1921 caaaagtagt gatgaagata ttggatacgg taaaattcaa aaaccattac aaacacccta
     1981 tcctccgatt tctgttccaa ttacaggata taattcaaat tcggcgaaag tagctgctaa
     2041 atacggattt tcccccttca gccactcaat aacttcctta cccgttctga aagataattg
     2101 gaaaacctat tcaagtgcca gcagattggg aaataccgag cccgacagat ctaattggaa
     2161 acttgcacgg accatattta tcgctgattc tgacaaagaa gcaatcaaaa aggcaagaaa
     2221 taacagtata tccaaggcct ataaatactt ggcaaccgcc ataaagaaag gacccggcct
     2281 agccattatg aaatcagacc caaacgaccc cgatagtgaa ataactgaag attacctcat
     2341 caatgaacta gtcattgctg ggtcagttga aactgtaata agtcgtctgc aagaagtcac
     2401 agaatacctc gggcctttcg gaaccctgat aaatatgtca ttcgactgga tagatgaatc
     2461 tgataaaaga gactggttaa acagtatggc tctttttcaa aatgaagtaa tcccaaagct
     2521 aagttcccaa atcaaccaat taattgctcc ggagaattca aatgactaat aactatcggc
     2581 aaaaccgcac aaaatccaat ttattaaaag gcaaaaccat taaagtaccc gaaataaata
     2641 gggttttcga ccctacggta gtcgaaacaa tcgcatcagt cggttacgag tgcgtttgga
     2701 tagacatgga acacagccac gcagatttca aagacctcgc aacactcgtc ttagcagcgc
     2761 gtgcctcgga aatagatgtg ttcgtaagaa tacctcacgg accttacaac caaataataa
     2821 aaaccttgga aattggcgca tccggtttaa tatggcctca ctgtaaaagt aaaaaagagg
     2881 ccgaagcgtt cgttgaaatg gccaagtatc atccagaagg catgagaggc atgggtggtg
     2941 gcagggattc ctccttcggt aaaagtgaaa agccagcgta ttacgacata gcgaatgaaa
     3001 atgtgctgct tggtgtgatg atagaagaca aggaaggggt ggaggcctgc ggagagatcg
     3061 cttcagttcc tggtattgac ttactttttg tggggcccgg agacctatcc caaagctatg
     3121 gtgtccacag agaccccgga aacccaataa gtcaacctat catattgaag gcttttgaca
     3181 aggttggttc agcctgtaag ttgcacggca agtttatggg cacagcagta gatccagggg
     3241 aaccgatggc tatggcagtg gcaagggggg ccacttggtt aaattgctgc catgacgtaa
     3301 ctgctctcac aaccggttat gaatctgcta tggtaaagac agaccaaatc gtggcttcca
     3361 caaatcagat ttaggaaaga ttcaccgaac ctctatagtc tgatttggct ttttcaatag
     3421 cacggattct accgtccaaa agaacatcta ccctgtcttt ggcccactgg tttgaaactt
     3481 ccaaacctgt aagagaacct tgaaattgag gcatcacata tctcgctaga agctcatagc
     3541 tacgtagaat actgtcactc ggagcccaat caatggtttg taccaggaat cccccaaatc
     3601 catcactctc tcgagccaaa ttattaatgc cttcaataca atcatcaggg gttccgacta
     3661 tccaaaatcc attatcagac atccaatcga cgacttgatc tctaggtccg tcaaagactg
     3721 gcttcctccc attggtacct tcagaataat cccttaaaaa ccctgcagcc ccttccctag
     3781 cttgctcccg ggccttctct ctggtctctg ccaaatgtac cggtactacc aatcgccaat
     3841 cctctctttt cacaactttc ccatgttgtt cagctgactc ttctgctata tcccacaatt
     3901 ttccatagtc tgctcggtcg gccagtggat cccttggtac ggtgatagtc aaaactgaag
     3961 caccgtgttt accggccagc tgaaccccgg agggagattg gatagatgca acggcaatcg
     4021 gcatatgtgg actctgataa ggtctgagct gcagcaaagc ctcatttaat tcaaaccaat
     4081 cgctcttata tgttattggt tcgatttctg taaataacct caatatgacc cctagggcct
     4141 catccatctt ttctctctgg gtagtgggat ctatgcccat catataggca tctgtaggca
     4201 aggccccagg acccacacca aacatgaccc tacctcgagt catatggtcc agctggacca
     4261 ttctattggc gaccataaaa gggtgatggt agggaagact tacgactcca gtaccgaggc
     4321 ttatacgcct cgttctttct gctactgcag ctatgaatag ttcaggagat gaaatagttt
     4381 cccagcccgc actatgatgt tctcctatcc atgcctcatc atatcccagc ttatctagcc
     4441 tcccagctta tctagccatt gaatcaactc catatctctc tctaaggcca aagttggatt
     4501 ttcacccacc ttatggaaag gagccaaaaa tatacaaaac ttcatctttt caggtagatt
     4561 catatcacat atcctccgca cttcattaat cttgatattt cataatatat gagatcatat
     4621 gggagtgcct taatcatgac aaataaaccc gcgcatcgtt ttttaatgac ggatgggctg
     4681 attttaaaag atctctaaga ataatttttg aaacctaaat ttttttacgg ctggtggatt
     4741 gcattaaccg ctttggtggt gaatgctgtg atttctgcac ccgcatttgg ggcaacagga
     4801 ctttggatcg attccctgga gactgaattt gggtggagtc gtactcaact atctatcgct
     4861 ttttcccttg gccaacttga aggaagtgtc gccgcccctt ttgttggcta cctaatcgac
     4921 aaaattggtg gtaagaaagt agcctgcatc ggggcaatcg cttcatccgt gggatttctt
     4981 tgtttgagtc aaacagttcc aataactgaa tccagggaaa attggctcga cccggctata
     5041 ttttacattt catacgtaac aataatgttc ggtgtaactt taggtggttg gataccgatg
     5101 acggtaatca taaacaattg gtttgccaaa cacagaagct tagctatgtc gatcggatct
     5161 gtcggcttct caatcggcac cttcgccatt gttccctttt tggccatctt gattgaatcg
     5221 gaaagtgtcg gctggaaaac gacagcacta ggacttactc tattttttcc tctcctaata
     5281 tttcccatca caagaataat taaaaacact ccagaagaaa ttggagaggt tcccgacgga
     5341 atagaagcta catcgactgt caacagcatc ccagatcaat ctgtggaaat aggatcaaat
     5401 gatttttctt tgaaagctgc tttacaggaa aaagtgtttt ggtacatagc aatcggccac
     5461 ggagcttctg caatgcttac ttccactatg atggttcact tgatcttagc atttaaacat
     5521 caaggcataa gtctacaaac ggcggcattt atttgggggc tggctatggg agtcggcggg
     5581 gtgtcacaaa tcttaggtgg attcttagga gataggacaa aaaaacatgt tgctgtttgt
     5641 atttttggtt gtatccaagc cataggagtc tcccttgctg tcttggtcac aaacacagga
     5701 acggcattaa tatttgcaat cgtctatgga atagggtttg gggctcgtgc cccaataact
     5761 acttctatga gaggtgatta ttttggtcgt aaggcgttcg gcaaaataat gggagtttct
     5821 gccatgtcca tgatgcttat gaccatgttt ggtccaattt ttgcaggaag gctcttcgac
     5881 ctccaaggaa actatcaaaa tggtttcttg atcttggcct gttgcggttt cataggaagt
     5941 ctaattttcc tattagcaaa aaggccggtt ctccaaaaaa caggttagac gaaaacacag
     6001 tatggaatta tgttacggct acgatgcccg gctggttttc ctaatctaat attcccaaat
     6061 ctctaaatat aacttgagtt ccttccaaat ctgataggtc ttctatagtc aactttggct
     6121 tatggagatc agctaagggg gtgagtaaca tgtgagtttt tattccttca acaactgggt
     6181 attcaaatat ttgcccagtg aaatattgct gaacaacttt tgag
//
LOCUS       DASCNS010000050         2606 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000419560, whole genome shotgun sequence.
ACCESSION   DASCNS010000050 DASCNS010000000
VERSION     DASCNS010000050.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000419560"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="QF838_02050"
     CDS             <1..1308
                     /locus_tag="QF838_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HJO59508.1"
                     /translation="PDTAKKISQVLKLARSNSIPVVARGAGTGLSGGAIAEHGGIVLS
                     MARMNQILEVNRSDRYAIVEPGVVNLDLSTHVASEGLYYAPDPSSQRACTIGGNIAEN
                     SGGPHCLAYGVTTNHVLGMEVVLADGTITWFGENERDGIGYDLRGITIGSEGTLAIVT
                     KISVRLLRLPEHVKTMLMSFETIEDASAAVTGIIGAGMVPAALEMMDHFCIKAAEGAV
                     GAGYPEGAGAVLLVELDGLLESVEEESEEIEAICREFSPLEIRVATDSLEREKLWSGR
                     KGVLGALGRLAPNYYLVDGTIPRTKLVEVLKKINEISERSGYPIANLLHAGDGNLHPS
                     ILFDERKPGDVENILAIGAEILRICVDSGGVLSGEHGIGLEKQDQMPLMFSEEDMATM
                     SLLRLAFETEDLLNPGKVFPTGAGCGDISQARAIARVGPGAYI"
     gene            1330..>2606
                     /locus_tag="QF838_02055"
     CDS             1330..>2606
                     /locus_tag="QF838_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJO59509.1"
                     /translation="MEEKHLIDGIQPNQGLIYPEDTNELSSVLSDITEPGRVVVPIGG
                     ATRLQIGAKMPTYDYALDLSNMNHLVAHNSADLTCIVQSGITLHSLQNQLREQEQFLA
                     VDAAFPGKATIGGTLASNAPGYLRWQLAHMRDMVIGMQVVLPSGTVTKSGGPVVKNVS
                     GYDMARLHVGGYGTLGVISEISFKLTPLPRRQGSFLVGFESEHGLQNFSEEIFDSYVM
                     PLALTGMNENLIKMLQIDAPQCQYLMVIRIGGRQMAFDRQLNILNKMVGQYQDSQTDF
                     LEQNASDRLWEKVRDFKGYGAISSITGRVFSTPTRLRGLLRDIDNYVEEIGLTLAICI
                     QPGFGTMEFHIDKVPAESEVRSTQMIERIHDIARQNQSHINYETLPADFKNGFNMWGK
                     VNEGSLGIMRSLRDTYDPGKKLNRGRYLTDKED"
BASE COUNT          772 a          446 c          683 g          705 t
ORIGIN      
        1 ccggatactg cgaagaagat atcccaagtg ttaaagctgg ccaggtcaaa ttcgattcct
       61 gttgtggcca gaggagcagg gactggcctt agtgggggag ctatagcaga acatggtggg
      121 attgttttat ccatggcgag aatgaatcag attttagagg tcaatagaag cgatcgatat
      181 gcaattgtgg agccgggtgt cgtaaaccta gacctatcca ctcatgtggc ttctgagggc
      241 ctttattatg ctcctgaccc atctagtcaa cgtgcatgca caatcggagg aaatattgca
      301 gagaattccg gcggcccaca ctgtttagcg tatggtgtta caacaaatca tgttttaggt
      361 atggaagttg tattagcaga cggaactata acctggttcg gagaaaatga gagagatggg
      421 atcggctatg accttcgagg aataactata gggtcagaag gtaccttggc aatcgtgacg
      481 aaaatatctg ttcgattgtt gagattgcct gagcatgtga agaccatgtt gatgtctttc
      541 gaaacgattg aggatgccag cgcagctgtt accggaataa ttggtgctgg gatggtacca
      601 gctgccttgg aaatgatgga tcatttttgt atcaaggcag ctgagggtgc ggtaggagcg
      661 ggataccctg aaggggccgg ggcagtcctt ttagttgagt tggacggtct gttggaatct
      721 gttgaagagg aatcagaaga aatagaagct atatgccgtg aattcagccc tctggaaatt
      781 agggtggcaa cggattccct cgaaagagaa aaattatggt caggacggaa aggtgtatta
      841 ggagcccttg gtaggttggc accaaattat tatttggttg acgggactat accaaggaca
      901 aaattagtcg aggttttaaa gaaaataaat gaaatatcgg aaagaagcgg gtacccaata
      961 gctaatttgt tacatgcagg cgatgggaac ctccacccgt ctatattgtt tgacgaaagg
     1021 aaacctgggg atgttgaaaa catactagct ataggagcgg aaattctgag gatttgtgtg
     1081 gactcgggag gggttttgtc tggcgaacat ggaattggtc tggagaaaca ggatcaaatg
     1141 cctttgatgt ttagtgaaga agacatggca actatgtccc ttttaagatt ggccttcgaa
     1201 actgaagatc tgcttaaccc gggcaaggtt ttccccactg gggcgggctg tggggatatt
     1261 tctcaagcta gggcaattgc gagggtaggc ccaggcgcct atatatagat agcaggtatc
     1321 caatagaaaa tggaagaaaa acatctaata gatggcattc agcccaacca aggactgatt
     1381 tatccagaag atacaaatga actttcttca gttttgtctg acatcactga accaggcagg
     1441 gtcgtagttc ctattggtgg ggcaaccaga cttcaaatag gtgcgaaaat gccaacctat
     1501 gattatgctt tggacctttc caatatgaac catttggtgg cccataattc tgctgatctt
     1561 acctgcatag tccaatctgg tattacttta catagcttgc aaaatcaact gagggaacaa
     1621 gaacaatttt tggcagttga tgctgctttt ccgggaaagg ccacaatcgg aggtacttta
     1681 gcatccaatg caccaggata cttgcgatgg cagttagctc atatgcgtga catggtaatt
     1741 ggcatgcagg tcgttttacc atcagggaca gtaaccaaaa gtggtggtcc agtggtcaaa
     1801 aacgtatcgg gatacgatat ggcaagatta catgttgggg gctatgggac attaggggta
     1861 atatcggaaa tatcgtttaa attgactccc ctaccccgca ggcaaggttc atttttggtg
     1921 gggtttgaat cagaacatgg gctgcaaaac ttttcagaag aaattttcga tagttatgtg
     1981 atgccgttgg ctttaactgg aatgaatgaa aatttgatta aaatgttaca gattgatgct
     2041 ccccagtgcc aatatttgat ggtcatcaga attggtggga ggcaaatggc atttgatcgc
     2101 caattaaata ttttaaacaa aatggttggt caatatcagg attcccagac agactttctg
     2161 gagcaaaatg catctgatcg gttatgggaa aaagtcagag attttaaagg atacggagca
     2221 atttctagta ttactggaag ggtgttttca accccaacaa gattacgtgg cttattgcga
     2281 gatattgata attatgttga agaaattgga ttgactttgg ctatttgtat tcaacctggg
     2341 tttgggacta tggaatttca tattgataag gttcctgcgg aaagcgaagt taggtcaact
     2401 cagatgatag agcgaattca tgacatagct cggcaaaatc aatcacacat taattatgaa
     2461 acattgccag cagattttaa aaatgggttt aatatgtggg gcaaggtaaa tgaaggctct
     2521 cttgggatta tgcgatctct aagagataca tatgacccag gtaagaaatt aaatagaggc
     2581 cggtacttaa cagataaaga agatat
//
LOCUS       DASCNS010000051         1194 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000438453, whole genome shotgun sequence.
ACCESSION   DASCNS010000051 DASCNS010000000
VERSION     DASCNS010000051.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000438453"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="QF838_02060"
     CDS             <1..449
                     /locus_tag="QF838_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59510.1"
                     /translation="IFFAVQELVHVMMNPFGGRLGDKFGYLLTVVAGMVVIAGAISYI
                     PSAKHSPILLTTASLIGLAQALIFPSTLAMISIRFTGQGIATGMAISGSFKNAGKVAG
                     PIIAGLLITMFDYEVTLRGMGLLLASSALVLLVSLRLGYDPTRIRM"
     gene            complement(478..>1194)
                     /locus_tag="QF838_02065"
     CDS             complement(478..>1194)
                     /locus_tag="QF838_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HJO59511.1"
                     /translation="AANSSPEEEDDQLSIASNIGMNRFIWDMRYPPANKVPDDKTTAD
                     RVVSGPVAPPGRYTVVLKCSGTEKSKTFEIIKDPRVEASQKELEDQFNLLLSIRDRLS
                     ETHDSINRIRSVCSQVDEWAQRAIGHSSETLVAEAAKKIMEKLSTIESELIQTEYQGA
                     RDRLNLPTRLNSKLSEITSVVSAGDFAPTRQSYEVFQVVSDEIEPHLLTLQNTIDEDV
                     KDFDNLVQELGIPAVVVRPQ"
BASE COUNT          285 a          276 c          263 g          370 t
ORIGIN      
        1 ggatattttt tgccgtgcaa gaactagttc atgtaatgat gaaccctttc ggcggtaggt
       61 tgggagacaa gttcggatat ctattgaccg tagtggcagg gatggttgtt attgcaggtg
      121 cgatatctta tataccttca gccaagcact cgcctatcct cttgacgacg gcctcactaa
      181 ttggcttggc ccaagcttta atattccctt caacattagc catgataagt attcgcttca
      241 ccggacaagg catcgccaca ggaatggcga ttagcggctc ttttaagaat gcgggaaagg
      301 tagccggtcc tataattgcc ggtctgctca tcacaatgtt tgattacgag gtgacactac
      361 ggggaatggg attgctccta gcctcatcag cgttggtttt gttggtctca cttagactag
      421 gatacgatcc aaccaggatc cggatgtagt ttgaacccgg atcctgataa ctgtgattta
      481 ctgaggtctc accactactg caggtattcc cagttcttgt acgaggttat caaagtcctt
      541 tacatcttca tcaattgtgt tttgcaaagt gagaaggtga ggttcgatct catccgaaac
      601 tacttgaaat acttcatagg attgtctggt tggggcaaag tccccggccg aaactacact
      661 ggtaatttcg gacagtttgc tattcaatct tgtaggtagg tttagccgat ccctagcacc
      721 ttgatattca gtctggatca attcactctc tatcgtagat aacttttcca ttattttttt
      781 tgcagcctca gctaccaatg tttccgatga atggccgatt gctcgctgtg cccactcatc
      841 tacctgagaa caaacgctcc taatccgatt aattgaatcg tgtgtttcag ataacctgtc
      901 tctgattgac aaaagtaagt tgaattggtc ctcaagttcc ttctggctag cttccactct
      961 cgggtccttt attatctcaa acgtctttga cttctcagta ccggaacact tcaatacgac
     1021 tgtatatctc cctggagggg ctacgggacc agaaacaacc cggtctgcag tggttttatc
     1081 gtccggcact ttatttgctg gtggatatct catgtcccag atgaatcggt tcataccaat
     1141 atttgaagca atagaaagtt ggtcatcttc ctcttctgga ctactgttag ctgc
//
LOCUS       DASCNS010000052        10664 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000439612, whole genome shotgun sequence.
ACCESSION   DASCNS010000052 DASCNS010000000
VERSION     DASCNS010000052.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000439612"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="QF838_02070"
     CDS             <1..342
                     /locus_tag="QF838_02070"
                     /inference="COORDINATES: protein motif:HMM:NF020260.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HJO59512.1"
                     /translation="LNAFGMTPEPFGPNSYLIRNIPKVLAEADPRDSFHKMLSALSEQ
                     IEFKSWEDKAAYSLACHAAIRAGKKMSYREMSLLVKQLEFCLQPNNCPHGRPTMINMN
                     KNHLEREFSRI"
     gene            complement(357..1151)
                     /locus_tag="QF838_02075"
     CDS             complement(357..1151)
                     /locus_tag="QF838_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024091.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJO59513.1"
                     /translation="MLNYIFSIHIISRWKTIVVWSASMFLLALMFSGLYDSFKGEISD
                     MIDNAPKLMEAMIGPLPKDTVTPESWLGIELYGLLFPILLGVIAVSAGASAIGAEEES
                     GTIELILASPISRGRVVLEKSLGIMIQLAIVSGFLWVGIAIGSILFPFDVSLSNVLAA
                     TAMGWIFGTTVAYISMSAQALKGRKGLALGVGSGVVGLSYVMMVMSELLDGLNSLKYI
                     SLFNYYDGPSVLINGLNETSFAVMLVLSGLFLLVSLYGFYNRDVGI"
     gene            complement(1156..2055)
                     /locus_tag="QF838_02080"
     CDS             complement(1156..2055)
                     /locus_tag="QF838_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO59514.1"
                     /translation="MNSIKTDVPVEITGFSKTYGTKIAVDRLDLKVNEGEVFGFLGPN
                     GAGKSTTIRSILNFLTPSDGKISIFGLDSVKNSVAIKNHIGYLAGDISLYDSMSGYQL
                     FKYLTGIGKNTNWDYVNDLANRLQISPQAKIGTLSKGNKQKIGLIQAFMHQPKLLILD
                     EPTSGLDPLMKQVFYEMVLQMKHLGNSVFVSSHDLNEVQHICDKAAFIKSGRLIATEN
                     IRGAFEFSLRRYTITFPKLPELSVFEKIESVSEVTQDENNLTVTVKGDISEFIKTLSD
                     QDPIDLSEEETSLEDIFMKYYED"
     gene            complement(2064..2384)
                     /locus_tag="QF838_02085"
     CDS             complement(2064..2384)
                     /locus_tag="QF838_02085"
                     /inference="COORDINATES: protein motif:HMM:NF020317.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HJO59515.1"
                     /translation="MTLTQEGRHLVNDDPEMVLHAAHSSDLPDVHCMGLYAVLGDHDF
                     ITILEAPDNEAAARFSLELGVKVGVEIQTVPAIPVSRLDHKMEWPPTSQDLPSPSEPE
                     DSET"
     gene            complement(2461..2799)
                     /locus_tag="QF838_02090"
     CDS             complement(2461..2799)
                     /locus_tag="QF838_02090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HJO59516.1"
                     /translation="MISIFVTIQVKDGYAGQFTEASFGDSQGSVRDETGCYRFDILRN
                     AEDPNRFHLYEVYENESALDAHRNAPHYKKWRSTVESWFDGDVERVMMNTVFPSDQGW
                     KDQKPCLLNW"
     gene            complement(2898..4307)
                     /locus_tag="QF838_02095"
     CDS             complement(2898..4307)
                     /locus_tag="QF838_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HJO59517.1"
                     /translation="MNKSPLFKNIRSIAKEIANKNISPIEITKYYLTRLNELGPEYNS
                     VVTVTGELALKQAEAAEKEIMSGLYRGTLHGIPFGAKDLLATDGGIPTTWGAEPFRHQ
                     TFKYNATVIDKLCSSGAILVSKLAMIELAGGMGYRQPNASLTGPCRSPWDKNTWAGGS
                     SSGSGSAVGTGLVPFAIGSETWGSILSPANNCGVSGLRPTFGRVSRYGAMALSWSLDK
                     IGPLCLSADDCGIVLNQIAGPDPKDSSTSDKPYEYSVYSNQRKFKLAVLASASTGIDE
                     EVADNFKKSLNALSQFCEIEEINFPEYPYEAITRTIMLAESGAIFEEFAGNGTATHLT
                     APEDKYGPYARTAVLAKDYLKALRLRDEISQKIDPIMSKYDAVCGPTRGTPASKLEDE
                     FRGAIRGNAKDTLGALGNSVGLPAISVPNGFTKANLPTGIQFIGQVYAENAILNLADR
                     FQQETDWHLKHPAELLEEI"
     gene            complement(4300..4515)
                     /locus_tag="QF838_02100"
     CDS             complement(4300..4515)
                     /locus_tag="QF838_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59518.1"
                     /translation="MTKYGHEREKEIMWDLVNLHFDNLISDDQVEKIKESIAGILTDA
                     NEIRTAKLDDSFPLLNWYQPFRGDPNE"
     gene            complement(4512..5303)
                     /gene="mazG"
                     /locus_tag="QF838_02105"
     CDS             complement(4512..5303)
                     /gene="mazG"
                     /locus_tag="QF838_02105"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HJO59519.1"
                     /translation="MDTSLPFPDTFEGILQLVTQLRSTGGCPWDQEQTLHTLTPLLLE
                     ECYELVEATENEQDSERLEEIGDVLLHIAFQILISEESGSFFRSDVFETTMEKYIRRH
                     PHVFSDQTVDSIEDLKNNWEKIKRDEKGESRQYEMDGIPRNLPGLAYALKIQQRAARC
                     GFDWDDSHEIRQKILEELSEFEAATNCDQQEEEVGDLLFTVANMARHNGIDPEQALRN
                     SNRKFVSRFTNMEKLSEQAGQKFSDLPLKSQDDLWHQVKLEEREP"
     gene            5366..6058
                     /locus_tag="QF838_02110"
     CDS             5366..6058
                     /locus_tag="QF838_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HJO59520.1"
                     /translation="MYTRIFNNILQALFIICVPLLLITTNVRIVLNSATIYDYGFNKY
                     KIEKHTGIEFEQLQLAGKQIRDYFNNDLEWITINISLRGGNVPNLFNEREILHMYDVK
                     NLVKMVYMVQLYSAVFLSLGCVFMLFNSFTNGRILTMKYIGRGGVFAFSLVIAVSILA
                     IIGFDRLFLFFHLVSFSNDLWVLDPRRDYLIAMFPQGFFFDCTVVIAFLTLVEGVILS
                     LIPRAIRLAVKK"
     gene            complement(6070..6498)
                     /locus_tag="QF838_02115"
     CDS             complement(6070..6498)
                     /locus_tag="QF838_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HJO59521.1"
                     /translation="MDISSQEVEHFLNHNHNAVLSTIRLNGATQLSAVTVGFYKGGAI
                     FTTTKSRAKFVNLNRTPECSLLISNSNWRPYLVLEGRAVIFSEDTTDPTILKNILRTA
                     YRSAAGKEHPNWEDYDEAMKKDRRVAIFVTGENIYGTIEI"
     gene            complement(6520..6975)
                     /locus_tag="QF838_02120"
     CDS             complement(6520..6975)
                     /locus_tag="QF838_02120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HJO59522.1"
                     /translation="MRQISIREGSISDTPTISKFQQKMALETESKILDESTVRQGVES
                     VLNCSDKGFYIIAEVGSKIIGSLLVTFEWSDWRNGWFFWIQSVFVDTEYRRQGVYRLM
                     HSEVIRRSKANGNCCGIRLYVEKDNRNAQKVYRTLGMHETDYYLYEEEF"
     gene            7061..7765
                     /locus_tag="QF838_02125"
     CDS             7061..7765
                     /locus_tag="QF838_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008885015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HJO59523.1"
                     /translation="MEKVFSSFDEAVSDIFDGAVIMVSGFGGTGGTAQNLIVALRDHG
                     ASNLTIISNTAGLASLMGFGTLPGHTAVDCGILIDNDQVGKLIASYPVSPSPSRINSF
                     ETHFRKGKIALELVPQGTLAERIRAGGAGIPAFFTPTGVGTELSEGKEVREFDGRSYI
                     METALKADFALIKAHKADTLGNLSYRGTSQNFGAVMVRSSGISIVEVDEIVSPGEIES
                     HLINTPALFVDRIVER"
     gene            7802..8464
                     /locus_tag="QF838_02130"
     CDS             7802..8464
                     /locus_tag="QF838_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HJO59524.1"
                     /translation="MLDRLPREVIAMRVARELKDGDIVNLGLGIPSLCSQYVPEGRTI
                     LYHSESGVLNYGPMAEQGEEDDDLINASGQFLEPTPGMSFFHSADAFGMIRGGHIDVT
                     VLGALQVSEKGDLSNWMLPSRGIGNVGGAMDLAVGAKRVIVAMEHKGREGEPKVVSEC
                     TFPLTSPTCVSLIVTDIAVISVGQKGLILKETAPGWGLEDVQKETEPELIPADDLKEI
                     EL"
     gene            complement(8468..>10664)
                     /locus_tag="QF838_02135"
     CDS             complement(8468..>10664)
                     /locus_tag="QF838_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024947.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HJO59525.1"
                     /translation="VDLLNMDYETFINTSYLKQGDSNRFTSSRPTDRKKILADLLDLS
                     YYERLESASKQHSRQLQNDVAVQRSVIENKSSIVQKKEVLLEKLETYEKESRNLLSEE
                     QSLSSELEDYNLNRQTLLAEISNKKTVSDQIKTFEKEVDLMLVQEMRWKAELDELKTL
                     LSRSEEIQSSYKEYQNLRSEYGKSSTLMSDFHKINEEKIVIEKTIAIEKLKLESQIAN
                     EWNRINTDLQPLVDSIPTLLHEINLANTTLQSLETEIGKREHLLDKANAMSEKVTILQ
                     ISNKTLMTQMSDSRNRFDMINHAEAACPLCLQSVSDGSKNHIRDGLQREGETSKAEYE
                     SNLKKIQTLTERKKELITNNELEQSNLVQKKTGTEKLKFNLMNQLAKSEDSSIELSSI
                     KLNVAQMEEQLNSEQFHSEELKQLKTLGSKLKKLEDTEKKHSHLESRVEQLSPYLELH
                     AQLQGSKDRLISVTQSIEDTKSIREARQTQIENWKLELVRIGKMMAEKGDLEQAINLI
                     QSKIKEVKGQNLNAVTIREQTRYQIEEIYKAEEDIKSMGLEILKSNEEVQLYEELASA
                     FGRNGIQALMIERAVPMLENTAKELLARLSDNKMTIKLELQEGRTDRATGLPSEELTI
                     TISDEQGTRSYETFSGGETFRIDFAIRIAMSKLLASRSGSPLPILFIDEGFGSQDTMG
                     QERLIEVIQSISEDFEKIIVITHIDSMKENFNQQIEISKTESGSVFTLQ"
BASE COUNT         2808 a         2170 c         2170 g         3516 t
ORIGIN      
        1 cttaatgcct ttggaatgac tccagagcca tttgggccca attcctattt aatacggaac
       61 atacctaaag tattagcaga ggccgatcca agagattcat tccataaaat gttatctgct
      121 ttaagtgagc agatcgaatt taaatcctgg gaagacaaag ctgcctactc cttagcatgc
      181 catgcagcta tcagggcagg gaaaaaaatg tcctatcgcg aaatgtctct tcttgtgaaa
      241 caactagaat tctgtttgca acccaataat tgcccgcacg gtcgtccaac tatgatcaat
      301 atgaacaaga accatttaga gcgggaattt tctagaattt aggttaacga gcttggttaa
      361 atacccacat ctctattgta gaaaccgtac aacgaaacga gtaaaaacaa acctgataat
      421 actagcatta cagcgaaact tgtttcattc agcccgttta tcaatactga aggaccatca
      481 taataattaa aaagtgatat atatttcagg gaattcaagc catcgagcaa ctctgacatt
      541 accatcatga catagctaag cccaacaacc ccgcttccaa cccccaaggc aagtcccttt
      601 cgtcccttaa gtgcctgggc gctcatagat atgtaagcga cagttgtacc aaagatccat
      661 cccatagcag ttgcggccaa tacattgctc aaggacacat cgaatggaaa gagaatcgat
      721 cctatcgcaa tacctaccca tagaaatccg ctgacaatcg ccagttggat cattatccct
      781 aaagattttt ccagtaccac tcttcctctg cttattgggc tagccagtat aagttcaata
      841 gtgcctgatt cttcttcggc accgatcgct gaggctcctg ccgatactgc aattactcct
      901 agtaaaatag ggaacaatag tccgtaaagt tctattccca accaactttc gggggtgaca
      961 gtgtcttttg ggagtggtcc tatcatggcc tccattagtt tgggggcatt atctatcata
     1021 tcgctgattt cacctttaaa agagtcatag aggcccgaga acattagggc taaaagaaac
     1081 attgaggcgg accaaactac gatggttttc catcttgaga tgatgtgaat agagaatatg
     1141 tagttaagca tgaggtcagt cctcgtagta cttcataaat atatcttcaa gacttgtttc
     1201 ttcttcagaa aggtcgattg gatcttgatc agaaagggtt ttgatgaatt ctgaaatgtc
     1261 tccttttacg gtcacagtca agttgttctc atcttgggtt acttcagaca ctgattctat
     1321 tttttcaaat acacttagct caggcaattt tggaaacgta atagtgtatc tacggaggct
     1381 aaattcgaaa gctcccctaa tattttcagt tgctatgagt cttcctgact taatgaatgc
     1441 tgctttatcg caaatatgtt gtacttcatt aaggtcatgc gatgagacga aaacactatt
     1501 gccaaggtgt ttcatctgga ggaccatttc atagaaaacc tgcttcatta aaggatctaa
     1561 tccactagta ggttcatcca agatcaacaa ttttggttgg tgcatgaagg cctgaattaa
     1621 accaattttc tgtttattcc cttttgatag tgttcctatt tttgcttgag gcgagatttg
     1681 taaccggttg gccagatcat taacataatc ccaattagtg tttttcccta ttccggtgag
     1741 atatttaaac aattggtatc ctgacatgga atcataaagt gaaatatcac ctgccaaata
     1801 tccgatatga ttcttgattg cgacgctgtt tttcacgctg tctagcccaa atatgctgat
     1861 tttaccgtcg gacggggtta gaaaatttag tatggatctt attgtggtgc ttttccctgc
     1921 tccattcggg ccaagaaatc caaacacttc accttcattc acttttaaat ctagtctgtc
     1981 aacggcaatt tttgtaccgt aggttttgga aaaccccgta atttctacag gaacatcggt
     2041 ttttatcgaa ttcatactag tatttaggtt tcactgtctt cgggctcaga cgggctgggc
     2101 agatcttgac tagtaggggg ccattccatt ttgtgatcga gtctagatac aggtatagct
     2161 ggtactgttt gtatctctac tccgacttta acccctagtt caagagaaaa tctcgctgcg
     2221 gcttcgttgt cgggagcttc aaggatggtt ataaaatcgt gatccccaag aactgcatat
     2281 aaccccatgc aatgaacatc agggaggtcg ctagaatgag ccgcgtgcaa gaccatttct
     2341 gggtcatcat taactaggtg ccttccctct tgtgtaagag tcatcaataa aaaataagta
     2401 gaaattttat taccccctat gttgtacgtt agttaggaat caagggaata gatagggaac
     2461 ctaccaattc aggagacaag gtttttgatc cttccatcct tgatcagaag ggaagacagt
     2521 gttcatcatt actctttcaa cgtctccatc aaaccaggat tccactgttg acctccactt
     2581 tttatagtga ggagcatttc gatgtgcatc cagcgcggat tcattttcat aaacttcgta
     2641 taaatggaat ctattcggat cttcggcatt tcgtaggata tcgaatctgt agcaccctgt
     2701 ttcatctcga acagaccctt ggctgtcacc gaagctggct tcagtaaatt gccctgcata
     2761 accatctttt acttgtatcg tgacaaatat tgaaatcata attttcctcg gtgtaattgt
     2821 agaatttggt ggacctggat agctttagtc cactagatta tatcagcggg gaccatctaa
     2881 tccttgctac ttgtagctta aatttcttct aagagctcag ccgggtgttt taaatgccaa
     2941 tcagtttcct gttgaaatcg atcggctaag ttcaagattg cattttcagc atatacctgc
     3001 cctataaact gaatgcctgt aggtaaattg gctttagtga agccatttgg aactgagata
     3061 gcaggcaatc cgactgaatt tcctagggca ccaagtgtat cttttgcatt gccccgaatg
     3121 gcgcctctaa actcatcttc cagttttgat gccggtgttc ctctagttgg cccacacaca
     3181 gcgtcatatt ttgacattat cggatcaatt ttctgggata tttcgtctct taaccgtaac
     3241 gcttttaaat aatccttggc aagcaccgcg gtacgcgcgt acgggccgta tttatcttcc
     3301 ggcgctgtga ggtgggttgc ggttccatta ccagcaaatt cttcaaaaat ggcaccagac
     3361 tccgctaaca tgatcgttct agttattgct tcataggggt attctggaaa atttatttct
     3421 tctatctcac agaattggct aagagcattc aaactttttt tgaaattgtc cgctacttct
     3481 tcatctatgc cggtagaagc actagctaat actgccagtt tgaatttacg ttgattagaa
     3541 tagacactat attcataggg tttatcgctt gttgaagaat ccttggggtc tggtcccgcg
     3601 atttggttta gcacgatgcc acagtcatca gcagataaac agagtggacc aattttgtct
     3661 agtgaccagc taagggccat tgccccatat ctactcactc tgccaaaagt aggtcttagg
     3721 cctgacactc cacaattatt ggctggtgac agtatggatc cccaagtttc cgagcctata
     3781 gcgaagggta ctaagcctgt cccgactgct gaaccagacc cactggatga accgccggcc
     3841 cacgtattct tgtcccaagg acttctacat ggtccggtaa gggaggcatt tggctgcctg
     3901 taacccattc cgccagcgag ttcaatcata gctaattttg aaactaatat tgcccctgaa
     3961 gagcacaatt tatctatcac tgtagcgttg tatttaaagg tttgatgcct gaatggttca
     4021 gcaccccaag ttgttggaat acctccgtct gtggccagta aatccttcgc tccaaaggga
     4081 atgccatgta aggttcccct atatagaccg gacattattt ctttctcagc cgcttctgcc
     4141 tgcttcagag ctagctcacc tgtgacggta actacggaat tatattccgg ccccagttca
     4201 tttagccgag ttaggtagta ctttgttatt tctataggcg aaatgttttt gtttgctatt
     4261 tcttttgcta tagaccgtat atttttaaac agagggctct tattcatttg gatcgcctct
     4321 gaatggctgg taccaattta gcagtggaaa gctatcatcc aatttggctg ttctaatttc
     4381 attggcgtcg gtaagtatgc cagctatgga ttccttaatc ttctcaactt gatcgtcaga
     4441 aatcaggttg tcgaaatgta gattcaccaa gtcccacatg atttcttttt cgcgctcgtg
     4501 gccatattta gtcatggttc cctttcctct aacttcactt gatgccataa atcgtcctga
     4561 cttttcaatg gcaaatcaga gaatttttgt cctgcctgtt cggataattt ttccatgtta
     4621 gtgaacctgg aaacaaactt tctattggag tttctcaatg cctgttctgg gtctatacca
     4681 ttatggcggg ccatatttgc gactgtaaac aacaaatctc cgacttcttc ttcttgttga
     4741 tcacagttgg tagcggcttc gaattcagat aattcctcca atattttttg acgaatttca
     4801 tgagagtcat cccaatcaaa accgcacctc gccgctcttt gttgtatttt cagtgcatag
     4861 gcaagaccag gcagattgcg aggtatacca tccatttcgt actgtctgga ttctcctttc
     4921 tcgtctcgtt ttattttttc ccagttgttc tttaagtcct cgatagaatc cactgtttgg
     4981 tctgaaaaaa catgagggtg tcgacggatg tatttttcca tagtggtttc aaaaacgtct
     5041 gacctgaaga atgaaccgga ttcttctgag attaggattt ggaacgcaat atgcaacagg
     5101 acatcaccta tttcttcaag tcgctcagag tcttgttcgt tttcggtggc ttctaccaat
     5161 tcgtagcatt cttcaagcaa tagtggagtt aaagtatgta aggtttgctc ttggtcccat
     5221 gggcatccac ccgtggaacg gagttgagtc actaattgga ggattccctc aaaagtatca
     5281 gggaaaggta gtgatgtatc catttttata tcctataggg cctttgttga cactctttag
     5341 ggcaggtcaa taagcttagt accgtatgta cactagaatt ttcaataaca ttctacaggc
     5401 cttgtttatt atttgcgttc cccttttact cataaccacc aatgtcagaa tagtattgaa
     5461 ttcagccaca atatatgact atgggttcaa caagtacaag attgagaaac atacggggat
     5521 agaattcgaa caattacaat tagccgggaa gcaaataaga gattatttca acaatgattt
     5581 ggaatggatc acaattaata tctcgctacg cggagggaat gtccccaatt tgtttaatga
     5641 gagggagatt cttcacatgt atgatgttaa aaatctcgta aaaatggttt acatggtgca
     5701 attatattca gcggtattcc tttcattagg ttgtgttttt atgcttttta attcttttac
     5761 taatggaaga attctgacta tgaagtatat cggcagaggg ggggtgttcg cttttagttt
     5821 ggtgatagca gtttcaattt tggctattat tggatttgat aggttatttc tgtttttcca
     5881 tttggttagt tttagtaatg atttatgggt tttagaccca agacgtgact atttaatagc
     5941 catgtttccg caaggattct tcttcgactg tacggttgtt attgcctttc tcacgttagt
     6001 ggagggagtg attttgagtt taataccccg ggccatccga cttgctgtga aaaaatagtt
     6061 taggcttcct taaatttcga ttgtgccata aatattttct ccagtaacga atatggcgac
     6121 tctcctatct tttttcatag cttcatcgta atcttcccaa tttgggtgtt cttttcctgc
     6181 agctgatctg taggctgtac gaaggatatt tttcaaaatt gtgggatcag ttgtatcttc
     6241 ggaaaagata acagctctac cttccaagac tagatagggt ctccaattcg aattcgaaat
     6301 aagtaaggag cattcaggag tcctatttaa gtttacaaat ttagctctag attttgtggt
     6361 agtgaatatc gcgccgcctt tatagaatcc aactgtgacg gcactaagtt gagttgcacc
     6421 attcaaccgt attgttgaca ggaccgcgtt atggttgtga tttaggaaat gttctacctc
     6481 ctggctactg atatccatta tcctgcctct ttggcatgat taaaattctt cttcgtatag
     6541 gtaataatca gtttcatgca tacctagggt cctatatact ttttgggcgt ttctattatc
     6601 tttctcaaca tacaacctga tcccacagca attcccattc gctttgctac gtcgaatgac
     6661 ttcactatgc ataagccggt atactccctg tcttcgatac tctgtatcga caaacacact
     6721 ttgtatccaa aagaaccaac cattcctcca gtcgctccat tcaaacgtta ctaataagct
     6781 tcctatgatt tttgaaccta cctccgcgat tatatagaag cctttatcag agcagttcaa
     6841 aactgattcg accccttgtc gaactgtcga ttcatctaaa attttagatt ctgtctccaa
     6901 ggccattttc tgctgaaatt tagagatggt aggagtatca gatatacttc cctctcttat
     6961 tgaaatttgc ctcattattg acctctctta ttgaatctgt atattagaca tgattatcgt
     7021 gcatttgctg aaaaccgaat cttctaggag tatacgatag gtggaaaaag tattcagttc
     7081 atttgatgag gctgtatcag atatttttga tggtgctgtc attatggttt ctggctttgg
     7141 aggtacgggt gggacagccc aaaacctaat tgttgctctg agagatcacg gtgcttcaaa
     7201 cctgaccata ataagcaaca ctgcaggtct tgcaagcctt atggggttcg gcactttacc
     7261 ggggcacacg gctgttgact gcggaatctt gatcgataat gatcaagtgg gtaaactcat
     7321 agcttcttat ccggtttctc catctccttc taggataaat tcatttgaaa cccattttcg
     7381 gaaagggaaa attgctttgg aattggtacc acaaggtact ctggctgaac ggatacgagc
     7441 tggaggtgca gggataccgg catttttcac tcctactggt gtgggaaccg aattgtccga
     7501 agggaaagaa gtgagggaat ttgatggtcg cagttatata atggaaactg ctttgaaagc
     7561 agatttcgca ttaattaaag cacataaagc ggatactttg gggaatctgt catacagagg
     7621 cacttcgcag aattttggtg ctgtcatggt cagatcttct ggtatttcta tcgtagaagt
     7681 ggatgagata gtaagccctg gtgaaattga atcacattta ataaatacac cagctttatt
     7741 cgtagataga atagtggaga gatgaagtga actcaatata tttctgagag agttaataac
     7801 gatgcttgat aggttgccga gagaagttat agcaatgaga gtagcccggg aactaaagga
     7861 tggcgatata gtcaacctgg gattgggtat accttcactg tgttctcaat atgttccgga
     7921 agggagaacc attctctatc acagcgaaag tggagtcctt aattacggac ccatggcaga
     7981 gcaaggggaa gaagatgacg acttgattaa tgccagcgga cagtttcttg agcctacccc
     8041 tggaatgtct ttcttccatt ccgccgatgc atttggaatg ataagaggag gtcacattga
     8101 tgtcactgtt ctgggtgccc tacaggtttc agaaaaggga gatctatcaa attggatgct
     8161 tccatctaga gggattggca atgtcggcgg agctatggat ctagccgtag gagcgaaaag
     8221 agtaatcgtt gcaatggaac ataaagggag agagggggaa cctaaggtag tatctgaatg
     8281 tacttttcct ttgactagtc ccacatgcgt cagcctgatt gttacagata ttgctgttat
     8341 ttctgtgggg caaaaaggtc taattttaaa agaaactgcg cctggctggg gattggaaga
     8401 tgttcagaag gagactgaac ccgagttgat tccagccgat gatttgaagg aaatagaatt
     8461 atagctatta ctgcaacgta aaaactgaac cggattctgt ttttgaaatt tcaatctgtt
     8521 ggttgaaatt ttctttcatt gaatcaatgt gagttatgac tatgatcttt tcaaaatctt
     8581 cgctgataga ttgtatgact tcaataagcc tttcctgtcc cattgtgtct tgcgagccaa
     8641 acccctcatc aataaataat attggtaatg gagaccctga cctggaggca agcaattttg
     8701 acattgctat tctgattgca aagtcgattc gaaaagtttc ccctccactg aatgtttcat
     8761 aacttctagt gccttgttcg tcagatatag ttatggttag ttcttctgag ggaagtcctg
     8821 tggccctatc tgttctgcct tcttgcaatt ctaattttat ggtcatttta ttgtcactaa
     8881 ggcgagccaa cagttcttta gcggtatttt ctagcattgg aacagctctt tctatcatta
     8941 atgcttgaat cccattgcga ccaaaagcag aagctaattc ttcgtacaat tgaacttctt
     9001 cattgctttt tagtatttct aatcccattg attttatgtc ttcttcagct ttatagattt
     9061 cctcaatttg gtagcgagtt tgctcacgaa ttgtcaccgc attaagattt tgtcccttca
     9121 cctccttaat ttttgattga attaaattga tcgcctgttc taaatcccct ttttcagcca
     9181 tcattttccc aattctgact agttccaatt tccagttttc tatttgggtt tgtctggcct
     9241 ctctaatgga tttggtgtcc tctatagatt gagttactga tataagcctg tcttttgacc
     9301 cttgaagttg cgcatgaagt tccaaatagg ggctcaattg ttcgactcta gattcaaggt
     9361 gactatgttt tttttcagtg tcttccaatt ttttaagttt agaacccaag gtttttagct
     9421 gctttaattc ttcggagtga aattgttcag aattaagttg ttcttccatt tgggccacat
     9481 ttaatttaat tgaactcagc tcaattgaag aatcctcact tttcgccaac tgattcatca
     9541 gattaaattt tagtttttct gtccctgttt ttttctgaac caaattactc tgctctaatt
     9601 cattgttagt aatgagttct ttttttcttt cagtaagggt ttggattttt tttaagttgg
     9661 attcgtattc agctttgctg gtttccccct cacgttgaag cccgtccctt atgtgatttt
     9721 tgctgccgtc tgaaacagat tgaaggcaaa gcggacaggc tgcctcggca tgatttatca
     9781 tgtcaaacct atttcggctg tcggacatct gcgtcattaa agttttattg gagatttgta
     9841 aaatggttac tttctcggac atagcgttag ccttatctaa taggtgttct cttttgccaa
     9901 tttcggtctc caaactttga agggtggtgt tggcaaggtt gatttcatgt aacaaagtag
     9961 gaatgctgtc cactaagggt tggaggtctg tattgatccg gttccattca ttggcaattt
    10021 gcgattctaa tttcagtttt tcaatcgcta ttgtcttttc tattacaatt ttctcttcgt
    10081 ttattttgtg aaaatcagac attaatgtag aagatttacc atactcagat ctcaagttct
    10141 gatattcctt ataagatgat tgaatctctt cagaacggct gagcaaagtt ttcaattcat
    10201 ctaattctgc tttccaacgc atttcttgga ctagcatcag gtctacttct ttttcgaatg
    10261 ttttaatttg gtcggatacg gtttttttat tggatatttc agctaataag gtctgacggt
    10321 ttaggttata gtcttccaat tcgctactaa ggctttgttc ttctgataat agatttcgag
    10381 actctttttc atacgtctcc aatttttcaa ggagtacttc ttttttttga acaattgaag
    10441 atttattctc gatcactgat cgttgcactg caacatcatt ttgcagttgg cgggaatgct
    10501 gtttagacgc tgattccaga cgttcataat atgagaggtc cagtaagtcg gctaaaatct
    10561 tttttctgtc ggttggcctg gaggatgtga agcgattgga atccccttgt tttagataag
    10621 aagtgtttat gaaagtttca tagtccatat tgagtaagtc gact
//
LOCUS       DASCNS010000053        11458 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000440300, whole genome shotgun sequence.
ACCESSION   DASCNS010000053 DASCNS010000000
VERSION     DASCNS010000053.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000440300"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="QF838_02140"
     CDS             <1..383
                     /locus_tag="QF838_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HJO59526.1"
                     /translation="FFLNPLTQKWEVTEDSITPLSFFDPEKGIEKILSSTTSPKFRSN
                     SEKYWNIEGSMPASSLSNLVGDTSDNNVEIIVWIDKDSLYLTRAIIFGQLNGYDDSEN
                     MNEIQRIIDISRFNEEIVIENPLD"
     gene            404..1939
                     /locus_tag="QF838_02145"
     CDS             404..1939
                     /locus_tag="QF838_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59527.1"
                     /translation="MKITNKNVALIPVFAGIFFAADDQTVVVTILPQIMSDLMVDVTE
                     LDRAMWTVTGYLLGYVSVMPIMGRISDIYGRKFIYVASMSVFLLGSVGTGLTNSMGFL
                     ESYSYHDSILIRSCVSSILETTTSINWVIATRIIQAIGAGALIPITIAIISDLYSGNK
                     RAIPLGLTGASAEAGAVTGPLWGGLITQSFSWQWVFWLNIPIGLLVIAGILFLVPNSK
                     KTTHKIDYLGSGIIVAAICFITLGCSQSGSNTSFLIIFISVGVSLLIGYYLLSKQNDD
                     SVIPIELFKNIRFLAANTLHLFYGAALIISLVTIPLMVNTVLSGSSLEGGLILMRMTI
                     AIPIGALIGGWLSKHYDLRIPAISGLILCFIALLFMSRWNELIGDPLMSYHLLAAGFG
                     FGLVIAPITTSAINSTGTRHQGSAAGIISSSRFLGMTFGIAALSAWGSQRFQDLLLGI
                     NLQIPGTGVLGNPTSGFEDQLIQAGVNLFHHFFIIGSVLCLIGIFPCLLLVKNRTSKD
                     PIK"
     gene            complement(1954..2790)
                     /locus_tag="QF838_02150"
     CDS             complement(1954..2790)
                     /locus_tag="QF838_02150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59528.1"
                     /translation="MEKDFEAELNMINLSGKEAIVTGGGSGIGRGICSVLALAGASVL
                     VTDISGKGAEDTLKHCGYGGKYRARQMDVTNENSIDTIISEHIEDRKKIDILVNNAGI
                     IGSEGWSNREIPNDQDWTEVYEVNVRGVVKVSERVSKEFQKQEHGKIINIASIAARQG
                     SPDSPHYNASKAAVVSWTQSHALRMAAYNVNVNAVCPGLIWTPIWESIARKRAKYGGS
                     LKTYGLEGKKLFDQTVKAWIPLQREQTPEDIGNMVAFLASDLAENITGQSINVDGGRF
                     TN"
     gene            complement(2763..3056)
                     /locus_tag="QF838_02155"
                     /pseudo
     CDS             complement(2763..3056)
                     /locus_tag="QF838_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015893.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(3133..4425)
                     /locus_tag="QF838_02160"
     CDS             complement(3133..4425)
                     /locus_tag="QF838_02160"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HJO59529.1"
                     /translation="MRNSIGVGLIGMGVVGSGVAKTITENSTVLHGHVGLPLNLVKVA
                     VRDTSKQRDFMVDQKILGDSPEDLINDESVNIVVELMGGEYPALEFILAAVKNGKHVV
                     TANKEVISKHGAEIFSLARDNNVRVLFEASVGGGIPIISPLMRDLVANDIKSIKAIIN
                     GTTNYILTRMANEQADYQEVLQEAQKLGYAESDPTSDVEGIDAAYKLSILSTLAFRAS
                     VKDTDIFIEGITNLTDKDFLYARELGYSIKLLAIATRNDSSVLGRVHPALIPEDQMLA
                     KVDDVLNAVEVETDLVDKVLFHGPGAGSLPTSSAVVADIVNIARTIADGTNLLDPVRL
                     SDEIEIKSIDTLVTKYYFRLEAEDQPGVLARIAAVLGDCGISISSFIQKGLGDHSSNA
                     ELVIMTHEASESSVTTAVASLRELDVVKMIGNVIRVVG"
     gene            complement(4435..5544)
                     /locus_tag="QF838_02165"
     CDS             complement(4435..5544)
                     /locus_tag="QF838_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJO59530.1"
                     /translation="MSSGRPLSVDKRRVAMQQPVLRRRQKLTIGLLGLSFLLVVSIFV
                     AGYIVVFVMPHRELVVKVDGVEYTRGDLVELVRIRQKSSEFMGGTFDASSGIFESLQL
                     MVENEVLMQMGPSQGIFVSDEEIDDQIALIMRPEKQMALGKSEEQIVRETSERHLNYL
                     NTIQIDEPTHRLLVQKAILREKFRQQVGESVPFLAEQVHLFRIVMPQTGEMDIMQVKY
                     EDAIRDITDPEQFQLAYIEIAREFSVDIPEKVRLGGDMGWIAKGVIPDYERDFFLLDP
                     GELSDPVKNKDNHTQTFFFMISERQPAKELSSDVRDELKSKALQDWVNSERSNHDVYA
                     IFNSDIYDWVFQQLRLSSRAPTPTPDPLQSILNSR"
     gene            complement(5605..5907)
                     /gene="rpsR"
                     /locus_tag="QF838_02170"
     CDS             complement(5605..5907)
                     /gene="rpsR"
                     /locus_tag="QF838_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HJO59531.1"
                     /translation="MTTERESTPQRRPGGRPRFYSRRKVCQPCVDKKELDYKDVDYIK
                     KFLSERYMIEARRKSGMCAKCQRSLAKAVKKARHLALLPFSPAQKGALPVHYRPRS"
     gene            complement(5961..6413)
                     /locus_tag="QF838_02175"
     CDS             complement(5961..6413)
                     /locus_tag="QF838_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HJO59532.1"
                     /translation="MTMNKILIIGNLGSDPEMRYTPNGNPVTSFTVATNRRYKTSAGE
                     NKEETEWFRISAWNRLAETSNQYLQRGSKVYVEGRLSSRTYVGNDGETRVSLDVNASE
                     VRFIDSRGGNTGSSGGFSEGGSIPDSTGSSPTVPSIEEGGEIEDDLPW"
     gene            complement(6438..6731)
                     /gene="rpsF"
                     /locus_tag="QF838_02180"
     CDS             complement(6438..6731)
                     /gene="rpsF"
                     /locus_tag="QF838_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013419.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HJO59533.1"
                     /translation="MAERKRKYELIMALSPVSTEAEATVIVDSIADVITSSGGEVTSR
                     EEWGVKRLSYLVNNFQEGSYYKADISMDSSDVKELERTLNANQDVLVHLISKM"
     gene            6906..7706
                     /gene="thyX"
                     /locus_tag="QF838_02185"
     CDS             6906..7706
                     /gene="thyX"
                     /locus_tag="QF838_02185"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HJO59534.1"
                     /translation="MAFKENPSVYLVTKPTINWSQIANFFEEENVPPIPDSVRAGDDE
                     SAAVIEISARMCYMSYGRGRRDITDFINNLLSSKDGSVFEHVNYGFIVTGVSRSLTHE
                     LVRHRAGFAYSQRSQRYVDETEGTFVVPPALSSEREFSKEARKVLDDALIHAAESYTE
                     LVTALEKSLPKSIFDSNTDRRKAIRQAARSVLPNATETKVFITANVRALRHFIEMRGA
                     IFADWEIRFLAIEILKLLQVEAPLLFGDFLVEDLDDGTQITYPKYSKV"
     gene            7789..8904
                     /locus_tag="QF838_02190"
     CDS             7789..8904
                     /locus_tag="QF838_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59535.1"
                     /translation="MSEIIEPRSKPKFDWFIPIDGDGEHIGTDKAEIPPTFDYLKKVA
                     LTAEENGFYSLLVPTRFSNGLFDENAPLAETWTTVAALAAVTEKIRFLVAVRPGFISV
                     GLFAQMAATLDQISGGRIDLNIVPGGIEGDMERLGEKADHDTRYERASEFISACKKLW
                     DKPEAINFNGEFINLNGAVCSPGPKGKPAFYLGGASEAALNLSADKADTYLAWILPQE
                     TLKPHLEKAQEKYMAKGRSPRFGLRTHLIVRQSEKEAWEIADELLAQTSKSVLKQRGK
                     SSENTSMVGQRSQSQKYKNNKMGTHLWNGISNVRVNCGTAIVGNVEQVSEELLKYWQL
                     GIDEFILSGFPHVTECNIVSEQLLPILIDKIDQELKI"
     gene            8901..9683
                     /locus_tag="QF838_02195"
     CDS             8901..9683
                     /locus_tag="QF838_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HJO59536.1"
                     /translation="MRWLRCQTKDEQFYGILNETIVSKVHGSPFDGYEQTTKKFPVEE
                     VKILPPVIPKTFYAAGVNYKKHVLEAASLLNREPNLPEKADIGYRANNAIIGHEDPIV
                     IPKDATDKIHYEAELVVVIGKIAKNVSMQDSLKYVLGYTVGNDVSERTWQASDRTLWR
                     AKNTDTFKPMGPWIETEAILDDMTTTVRLNGQETISFDTNDMIFGVEKYISEITRYLT
                     LYPGDVIWMGTDGTSKDMKNGDTCEIEISGVGTLSNPVIRDS"
     gene            9721..10677
                     /locus_tag="QF838_02200"
     CDS             9721..10677
                     /locus_tag="QF838_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HJO59537.1"
                     /translation="MSKIESVLGPMETSDLGFTLSHEHIVVSSAGIPQIYPEFIHRED
                     SIAEAIKVLSEAKDDGLDSIIDVTTLDLGRDIDMLAKVSKESGVNIICATGTWRDIPR
                     VFWNASIDSVASLYKREITTGIEGTNIKAGIIKVANDVGGVTREGEIILRAAARAQKE
                     TGVPISTHTWAPDRVGEQQVRIFEDEKVDLNRVYIGHSNDTMDVHYLESLLDKGVWIG
                     LDRYPGGRAMGTPNWEQRTDTAMKLIADGYGNRIMLGHDWSVRLSIDSKEGQTSRSKY
                     NPDGYLFITRQVIPRLKGLGASKEDIQNIMVDNPRKFFEGKS"
     gene            10911..11270
                     /locus_tag="QF838_02205"
     CDS             10911..11270
                     /locus_tag="QF838_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024093.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJO59538.1"
                     /translation="MIRFSGAIIWTYDLGRLRHFYEIKLGLTPHSVRENFIAYRWGEL
                     RFSIGSHSEITDDSIEPLRIMLNFDVKDIKTFATTLKDNGVKFLREPEEEHWGGWVCT
                     FQDPDGNIIQLLQHPDH"
     gene            complement(11282..11458)
                     /locus_tag="QF838_02210"
     CDS             complement(11282..11458)
                     /locus_tag="QF838_02210"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF019356.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HJO59539.1"
                     /translation="MAARKIGWVNVALMCAHEMKVPGALQKCIRVMVHVNTDKNPTDI
                     VNIYLRDAANLRKR"
BASE COUNT         3414 a         2514 c         2263 g         3267 t
ORIGIN      
        1 cttttttttt aaacccactt actcaaaaat gggaagtcac cgaggattcc ataacccctc
       61 tttccttttt tgaccccgag aaaggaattg agaaaatact atccagcacc acttccccaa
      121 aattcaggtc taatagcgag aaatattgga atatagaagg atctatgccg gcttcatcac
      181 tttctaattt ggtcggagac acaagtgata ataacgttga aataattgtg tggatagaca
      241 aagactccct ctatctaact agagcaatta tttttggtca attaaatgga tatgatgatt
      301 cagaaaatat gaacgaaatc caaagaataa ttgacatatc cagatttaat gaagaaatag
      361 tcattgagaa ccctttagac tagtatttat agctgaatac atgatgaaaa tcaccaataa
      421 aaatgtggct ctgatacctg tttttgcagg tatatttttt gctgcagacg atcaaactgt
      481 agttgttacg atacttcccc agataatgtc agacttaatg gttgatgtga ccgaactaga
      541 tagggctatg tggacagtta caggttacct attaggatac gtatctgtaa tgcctattat
      601 gggtagaatt tctgacatat atggccgcaa atttatctac gtagcttcta tgtcagtttt
      661 tttgctgggg tcggttggta ccgggttgac taactcgatg gggtttttag aatcttattc
      721 ctaccatgac agtatcttaa tcagaagttg tgtatccagc attttagaaa ccacaacgtc
      781 gataaactgg gtcattgcta ctcgaataat ccaagctatc ggggctgggg ccttaattcc
      841 aattacgata gcaatcatca gtgatttata ttctggtaat aaaagagcta tcccattggg
      901 gttgaccggg gcatccgcag aagctggtgc agtcaccggt cctttatggg gaggattaat
      961 cactcagtct ttttcatggc agtgggtatt ttggttaaac ataccaattg gattacttgt
     1021 aatagcggga attttatttc tcgtccctaa ctcgaagaaa accacgcaca aaatagatta
     1081 ccttggctcc ggcataatag tcgcagcaat ctgctttatc acattaggat gctcccaatc
     1141 tggaagtaat acatcatttt tgataatttt tatatctgtt ggggtatctt tgctaatcgg
     1201 ttactaccta ttatcaaagc aaaatgatga ttcagtcata cccattgaac tattcaaaaa
     1261 catccgattc ttagctgcga acaccttaca tttattctat ggagcagccc tcatcatcag
     1321 tttggtgaca ataccactaa tggtaaacac cgtcctatct ggcagctctc ttgaaggggg
     1381 gttaattcta atgagaatga caatcgctat cccaattgga gcgttgatag ggggatggct
     1441 tagcaaacat tatgatctca gaatacccgc gatttctggg cttatacttt gttttatagc
     1501 cctactattt atgagccgct ggaacgagtt aattggggac ccattgatgt cataccattt
     1561 attagcagct gggtttgggt ttggattggt gatagcgccg attacgacat ccgctatcaa
     1621 tagtactggt acaagacacc aaggttcggc agctggaata atcagctctt cacgattcct
     1681 gggaatgact tttggcattg ctgcactatc agcttgggga tctcaaagat tccaagattt
     1741 actactaggt ataaatttac aaattccagg aactggagtt cttggaaatc ccacgtcagg
     1801 gtttgaagat caattaatcc aagcaggggt aaatttattc catcatttct ttataattgg
     1861 ttcagtactc tgcttaatag gaatattccc atgccttcta ttggtaaaaa acaggacatc
     1921 taaagatcct ataaaataac atgggcaaac attttaattg gtaaaccgac caccatccac
     1981 gttaatggac tgccctgtaa tgttttctgc gagatcagat gctaaaaaag ccaccatgtt
     2041 tccgatatcc tctggagtct gctctctttg aagcggaatc cacgctttaa cggtttgatc
     2101 aaaaagtttt ttaccttcaa gaccgtaggt cttaagactg cctccatatt tagctctttt
     2161 tcttgcgatc gattcccata ttggggtcca tatgagcccc ggacaaacgg catttacatt
     2221 cacattatat gcagccattc gtaaagcatg cgattgtgtc caactcacaa ctgccgcctt
     2281 tgaagcattg taatgtggtg aatcggggct tccttgccga gcagctattg aagcgatgtt
     2341 aatgatttta ccgtgttcct gcttctgaaa ctctttgcta accctttcag aaacctttac
     2401 aacccccctt acattaacct catatacttc ggtccagtct tgatcattcg gaatttctct
     2461 atttgaccaa ccttcggatc caattattcc tgcattgttg accaatatat cgatcttttt
     2521 tctatcctcg atgtgttctg aaataattgt atcgattgaa ttttcattag taacatccat
     2581 ttgtcttgct ctatatttac cgccgtaacc acaatgtttc agggtatcct cagcaccttt
     2641 cccagatatg tctgtcacta acacgcttgc ccctgccaag gccaaaaccg aacatatacc
     2701 tctacctata ccgctacctc ctccggtgac gattgcttcc tttccagaca aattaatcat
     2761 atttagttca gcctcaaaat ctttttccat tctgaagtta caaatctttt gccctgatag
     2821 attacgatag gtcttttgat caacctgggt tcggcaatca tcagatccaa catttgagcg
     2881 gcagccacat ttgatttttc caaatctagc tttttaaaag atgggcttct ccaagaaaat
     2941 agctcggatg gatcatctcc taataaaaca ccctggagtt catccaagga aaaaggttca
     3001 ttgaaaaaat cccttacttc acactcaaat ccagcctgcg aaagctccgc cttcgctttt
     3061 gtacaactac ttcaacgatt ccaaccatag aacaacaaat gctgcattca aacctccaac
     3121 gatataagtc ggttacccga ccaccctgat cacattacct atcatcttaa caacatccag
     3181 ttcccttaaa ctcgctactg ccgttgttac agaactttca ctggcttcgt gagtcataat
     3241 gaccaattca gcattcgaag aatgatcacc caatcctttt tgaatgaaag atgaaatact
     3301 aataccgcaa tcccctagca cagcagcaat tctagcaagt actcctggct gatcctcagc
     3361 ttcaagtcta aaatagtatt tggttaccaa agtatctatt gatttaatct ctatctcatc
     3421 agataaacga accgggtcta gtaaattagt gccgtcggca atggttcgag caatgttgac
     3481 gatatctgcc actacggcac tgctggtggg cagggagcct gcgccggggc catgaaacaa
     3541 taccttatct accaaatccg tttctacttc cacggcattc aggacatcat ccactttagc
     3601 taacatttga tcctctggta tcaaagcagg atgaactctt cccagtacac tagaatcgtt
     3661 tcgagtcgct atagccaaca actttataga atatcctaat tctctagcgt ataaaaaatc
     3721 tttatcagta aggttggtta tcccttctat aaatatgtca gtgtctttca cgctagccct
     3781 gaacgctaag gtagataata tcgacaattt atacgccgca tctattccct ctacatcact
     3841 ggttggatct gattcagcat agcccagttt ttgagcctcc tgcagcactt cctgataatc
     3901 agcttgttcg ttggccattc gggtgagaat gtaattggta gttccgttga taattgcttt
     3961 aatagatttg atgtcattgg ccaccaaatc acgcatcaag ggagaaatta ttggtatccc
     4021 cccacccaca cttgcttcga aaagtactct gacattgttg tctctagcca aagaaaatat
     4081 ttcagctcca tgttttgata tgacttcttt gttcgcagta actacatgct tgccattttt
     4141 tactgctgcc agaatgaatt ccaatgctgg atattcacca cccatcaatt ccaccacgat
     4201 atttactgat tcatcattta ttaaatcttc aggactatct cctaaaattt tttgatcaac
     4261 catgaaatct ctttgctttg aggtatcacg aaccgctacc ttcactagat ttagtggcaa
     4321 tcctacgtga ccatgcaata cggtagagtt ttcagttatt gtttttgcca ctcctgatcc
     4381 gaccacaccc atcccaataa gtccaacccc tatagagttt ctcataaggc gcctttacct
     4441 agaattcaat attgactgaa gtgggtccgg tgttggagtt ggtgcacgag atgacaaccg
     4501 cagctgttga aagacccaat cgtaaatatc cgagttaaag atcgcataga catcatgatt
     4561 gcttctttcg ctattaaccc aatcttgtag agcctttgat tttagttcgt cgcgaacatc
     4621 agatgataat tctttcgccg gctgacgctc ggagatcata aaaaagaaag tctgggtgtg
     4681 gttgtctttg tttttgacag ggtcacttaa ttctccagga tctaataaaa aaaagtcccg
     4741 ctcgtaatct gggataactc ctttggcaat ccaacccatg tctcctccca acctgacttt
     4801 ttctgggata tctactgaaa actcgcgtgc aatttcaata taggctaact gaaactgttc
     4861 cggatctgtg atatctctta tggcatcctc atatttgacc tgcattatgt ccatctcacc
     4921 tgtttgtggc atcacaatcc ggaaaaggtg aacttgctct gctaagaatg gaaccgactc
     4981 tccaacctgc tgtctaaatt tttccctaag gatcgccttc tgtaccaaca atctatgtgt
     5041 tggctcatct atttgaatag tattcaagta gttcaaatgt cgctcagagg tttcccgaac
     5101 aatctgttcc tccgatttgc caagagccat ttgtttttcc ggacgcatga tgagagctat
     5161 ttggtcatct atctcctcgt cagaaacaaa aattccctga gaggggccca tttgcatcaa
     5221 aacctcgttt tctaccatca actggagaga ctcaaatatt ccggaactgg catcaaatgt
     5281 gcctcccata aattcggagc ttttttgcct tatcctcacc aattcaacca aatcacctct
     5341 tgtatattcg accccatcca ctttcaccac caactcccta tgtggcatta caaataccac
     5401 gatatatccg gcgacgaata ttgaaaccac caacagaaaa gataatccta agaggccaat
     5461 agttagtttt tgacgacgcc ttagcacagg ttgctgcata gcaacccttc ttttgtctac
     5521 acttaaagga cgaccagaac tcacttgatt taagactttc ctggaatgat taattattta
     5581 cacgagggaa aacatgcggg ctagttatga ccgaggcctg taatgaacag gtaaagcacc
     5641 tttttgggca ggcgaaaaag ggagcagcgc taagtggcgg gcctttttca cggctttggc
     5701 caaagacctt tgacatttcg cacacatacc actttttcga cgagcctcta tcatgtatct
     5761 ttcagaaaga aatttcttaa tataatcgac atccttgtag tccaattcct ttttatccac
     5821 gcaaggttgg caaaccttcc gacgtgaata aaagcgtggg cgacctccag gccttctttg
     5881 tggggtactt tctctttctg ttgtcattaa tagactccga tttagttact aaacttacga
     5941 aaagttaaaa ttctcctcgc ttaccacggc aaatcatctt cgatttcccc accctcttcg
     6001 atcgaaggaa ccgtaggaga agatcctgtg gaatcgggaa ttgatcctcc ctccgaaaac
     6061 cctcctgacg aacccgtatt accaccccta ctatcgatga atcgaacctc agaagcattc
     6121 acgtcaaggg acaccctagt ttctccatca tttccgacat atgttcggct actgaggcgg
     6181 ccttctacgt aaaccttact tccccgttgc aggtattgat tggatgtttc tgccaaccta
     6241 ttccaagcac tgattcgaaa ccactcagtt tcttctttat tctccccagc agaggtttta
     6301 tatcgtcgat ttgttgcaac agtgaatgag gttactgggt ttccgttggg cgtgtacctc
     6361 atctcaggat cggatcccaa gttcccgatt atgagtattt tattcatagt catatcaaac
     6421 cctgcctcta acgagaatta cattttcgat atcaaatgta ccaatacatc ttgatttgcg
     6481 ttcaaagtcc tttccaattc tttaacgtca gatgagtcca ttgaaatatc agctttataa
     6541 tagcttcctt cctgaaaatt gtttaccaag tacgaaagcc ttttcacacc ccattcttcc
     6601 ctgcttgtca cctctccacc tgacgaggtg ataacatccg caatactgtc cacaattact
     6661 gtagcctcag cctcggtcga aaccggggac agggccataa tcaactcata ttttcgtttt
     6721 ctctctgcca aagccagaac actccaatga ttattttttt gccaaccttc attatatcac
     6781 gctgaaggca cgctcaacaa ttctaatttc agcagaaaac cctgtctatt tcacccccct
     6841 gagaatgcaa atttctaagg ctaagtgata agattctttg aaactgaaag ttcctggaga
     6901 tattaatggc cttcaaagaa aatcctagcg tttatttggt cactaaacca actattaact
     6961 ggtcccaaat cgctaatttt tttgaagagg aaaatgtacc tccgattcct gattctgtga
     7021 gggctggtga cgacgaatca gctgctgtta tagagatatc ggcaagaatg tgttacatga
     7081 gctacggcag aggccgtaga gacataactg attttatcaa caatctactt agctctaaag
     7141 atggaagtgt atttgaacac gtaaattacg gtttcatcgt gactggagtt tctagaagcc
     7201 ttactcacga attggttcgg catcgagcgg gattcgctta cagtcaaaga tctcaaagat
     7261 acgtggatga aacagagggc acatttgttg ttcctccagc cctgtcctca gaaagagaat
     7321 tctcgaaaga agcgagaaaa gtattagacg acgcgttaat tcatgcagcc gaaagctaca
     7381 ctgaactagt cactgcttta gaaaaatcat tgccgaaatc aatatttgat agcaataccg
     7441 atagaagaaa agctataaga caagcagcca gatcagtttt gccaaatgca actgaaacga
     7501 aagtttttat taccgcaaat gtgagggcat taagacactt catagaaatg agaggggcca
     7561 ttttcgctga ttgggaaata agattcctcg caattgaaat acttaaatta cttcaggtcg
     7621 aagctccatt attatttggt gattttctag ttgaggacct agacgatggc acccagataa
     7681 catatccaaa atactcgaaa gtttaatcgt ccccatattt aattaggggt ttgagcctag
     7741 ttatgttcca caatgtaaaa aacccaccag ggtagggaga acgtgcagtt gtcagagata
     7801 atagagcccc gcagcaaacc taagtttgat tggttcatac ccatagacgg agatggagaa
     7861 catataggca cagacaaggc agaaatccct ccaacatttg attacctaaa aaaagtggct
     7921 ctcactgcag aagaaaacgg attttactca cttcttgtac ctacaagatt ttcaaacggc
     7981 ctttttgatg aaaatgcacc tcttgccgaa acctggacaa cagttgcggc tttagctgca
     8041 gtcaccgaaa aaataagatt cctcgtcgca gtgagacctg gattcatatc ggtaggatta
     8101 ttcgcacaaa tggccgccac gctggatcaa atctcaggtg gaaggataga tctaaacata
     8161 gtgccaggcg gtatagaggg ggatatggaa agattgggag aaaaggcaga tcatgatact
     8221 aggtacgaaa gagcttctga atttatttct gcatgcaaaa aattatggga caaaccagag
     8281 gcaattaatt ttaatggtga attcatcaat ctgaatggag cggtttgttc tcctggaccg
     8341 aagggtaagc cggcatttta cctcggcggg gcatcagagg ctgcactgaa tttgtcggcc
     8401 gataaggctg atacatattt agcatggatt cttcctcagg aaacactgaa gccccacctg
     8461 gaaaaagcgc aggaaaaata tatggctaaa ggacgatctc ctcgttttgg tctaagaact
     8521 cacttaatag tcagacaatc tgaaaaagaa gcatgggaaa ttgcagatga actactagct
     8581 caaacctcta aatctgtttt gaaacaaaga ggaaagtctt ccgaaaacac cagtatggta
     8641 ggtcagagat ctcaatccca aaaatataaa aataacaaaa tgggcacaca tttgtggaat
     8701 ggcatatcca atgtccgagt gaattgcggg acagccatag ttggaaatgt ggaacaggtc
     8761 tcagaagaat tgctgaaata ttggcagctt ggaatagatg agtttatatt gtccgggttt
     8821 cctcacgtta cagaatgcaa catagtttca gagcaattat tacctatcct tatagataaa
     8881 atagaccagg agttaaagat atgagatggc taagatgcca gacaaaagat gagcaattct
     8941 atggtatttt gaacgaaacc atagtatcca aagttcatgg atcaccattt gatggttatg
     9001 agcaaaccac taaaaaattt ccggttgaag aagtgaaaat actacctcct gttattccga
     9061 aaactttcta cgccgcaggg gtaaattaca agaaacatgt cttagaggca gcatccctgt
     9121 tgaacaggga gcccaattta cctgaaaagg ccgatattgg atatagagcc aataatgcaa
     9181 tcataggcca cgaggatccc atagtcatac ccaaggacgc aacagataaa attcactacg
     9241 aagctgagct ggtagtggtt attgggaaaa tcgccaagaa tgtttcgatg caagattctc
     9301 taaagtacgt tttgggatat accgtgggta atgacgtcag tgagagaaca tggcaagcct
     9361 ctgatagaac tttatggcgc gccaaaaata ctgacacctt taagcctatg ggaccttgga
     9421 ttgaaacaga agcaatcctc gatgacatga caacgaccgt aaggcttaat ggtcaggaaa
     9481 ccatttcgtt tgacaccaat gacatgattt ttggagtaga aaaatatatc tcagaaatta
     9541 cccggtacct aactttgtac cctggagacg tgatctggat gggaactgat ggtacgtcca
     9601 aggatatgaa aaatggcgat acatgtgaaa tcgagatttc gggtgtaggc acgcttagta
     9661 atcccgtgat cagggatagt tagactccgt caaaaaatac gactaaaggg agaaggtact
     9721 atgagcaaaa tagaatcagt tttgggccca atggaaactt ctgacttagg cttcacctta
     9781 tcgcacgaac acatcgtagt cagttctgcc ggtatacccc aaatatatcc agaattcata
     9841 caccgagaag attcaatagc cgaggcaata aaggttcttt ccgaagcaaa agatgacggg
     9901 cttgattcaa taattgatgt gactacactc gacctcggca gggatattga tatgttggca
     9961 aaagtgtcaa aggaatcggg tgtaaatata atatgtgcta ctgggacatg gagggacata
    10021 cctagagtat tttggaatgc ttctatagat agtgttgcgt ctctttacaa gagagaaata
    10081 acaacaggaa ttgaaggtac caatataaaa gctgggataa ttaaagtggc taatgatgtt
    10141 ggcggagtta ctcgagaagg agaaattata ctcagggcag ctgcacgggc ccaaaaagaa
    10201 accggggttc caatatccac ccatacctgg gcccctgatc gtgtcggcga gcaacaggta
    10261 agaatttttg aagatgaaaa ggttgactta aatagggtct acattgggca cagcaacgac
    10321 accatggacg tacattacct agaaagtcta ctcgataaag gtgtttggat agggctggat
    10381 aggtatccag gtggcagagc tatgggaact ccgaattggg agcaaagaac cgatactgcc
    10441 atgaagctga tagcagacgg ctatggaaat agaatcatgc taggacatga ctggtcagtc
    10501 aggctcagca tagattcaaa agaagggcag acgagtcgat cgaagtataa tccggacgga
    10561 tatttattca taacccggca agttattcct agacttaaag gcttgggtgc atcaaaagaa
    10621 gatatacaaa acattatggt tgacaaccca agaaagtttt ttgagggaaa aagctaaaag
    10681 cgcccgcagt agtaacctac tccgagcgct ttacatgtgg aggcgttcca gagaacacct
    10741 acctggtcga cctcctacta ctactatcca acgcgactta cctcctttta aaagaccctc
    10801 tgtaaacttt catccaccca gcgtttctgt cttctattct cattatatac aagggcttga
    10861 tgaaattgga aacgaattat tgactaaatg aagagtaaaa taaaggaatc atgatcagat
    10921 tttcaggggc aatcatatgg acatatgact tagggcgcct aaggcatttt tatgaaatta
    10981 aactaggcct tacccctcac tctgtgagag agaatttcat tgcctacaga tggggggaac
    11041 tcaggtttag cattggatcg cattcagaaa taacagatga ttccatagaa cctctaagaa
    11101 taatgttgaa tttcgatgtc aaagatataa aaacattcgc cactaccctc aaagataatg
    11161 gggtcaagtt tcttagggaa ccagaagagg aacattgggg cgggtgggtc tgcacatttc
    11221 aagacccaga tggaaatatc atccagcttc ttcaacaccc agatcattaa tcatcttcca
    11281 attatcgctt cctgagattg gcagcatctc gaagatatat attcacaata tcagtaggat
    11341 tcttatcagt gttcacgtgc accatgactc ggatgcattt ttgcagtgct ccaggaactt
    11401 tcatttcgtg agcacacatt agggcaacat ttacccatcc aattttccta gcagccac
//
LOCUS       DASCNS010000054         6872 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000460471, whole genome shotgun sequence.
ACCESSION   DASCNS010000054 DASCNS010000000
VERSION     DASCNS010000054.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000460471"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="lepB"
                     /locus_tag="QF838_02215"
     CDS             <1..617
                     /gene="lepB"
                     /locus_tag="QF838_02215"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HJO59540.1"
                     /translation="IYERKIRRAVREIVETSILAMFVFSVLYISIQNYQVEGSSMAPN
                     INQGDCVIANKAAYWKIPEPRLGNFSENNTSALKNRYLFGDPDTGDVIVFTYPEDPNR
                     HFIKRIIATEGDSIHINDGVVFLNSVLLEEPYLDPALIDSSNWGPNVVGIGEYFVLGD
                     NRRASNDSRDWGTLSRKHIIGKYLGRYPSSVCSISPMTQETYDK"
     gene            678..753
                     /locus_tag="QF838_02220"
     tRNA            678..753
                     /locus_tag="QF838_02220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Lys,seq:ttt)
     gene            complement(839..1627)
                     /gene="arsM"
                     /locus_tag="QF838_02225"
     CDS             complement(839..1627)
                     /gene="arsM"
                     /locus_tag="QF838_02225"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HJO59541.1"
                     /translation="MRLRSRCIETDGGKKLVDKELIIESVRYRYGELARGAADCCGER
                     DGFAPGSGVYESTQIKDLPEEALMASAGCGNPNAIGELKQGETVVDLGSGGGIDCFIA
                     ANMVGETGRVIGVDMTPDMINLANENKVKVEAGNVEFRLAQIDKTGIDTDSVDVIISN
                     CVIVLAPDKDAVFRESVRILKKGGRMHISDVMLTSELPDHVSSDMDNWTHCLAGAELI
                     DVYIKRMRESGFSKVEITSNVSYREGLEEDWVSSIRSVNLLATN"
     gene            complement(1587..1934)
                     /locus_tag="QF838_02230"
     CDS             complement(1587..1934)
                     /locus_tag="QF838_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJO59542.1"
                     /translation="MVISNNVLEFDTSLAAEGFSAIGSEPRLNVLIVLVKAGSEGLTV
                     GEVRERIGMPPSTLAHHLRILESEELIRQEKRGRAVLNFAVITHIEALAAFLLKECCQ
                     DEVEIKMHRNRWR"
     gene            2356..3549
                     /locus_tag="QF838_02235"
     CDS             2356..3549
                     /locus_tag="QF838_02235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJO59543.1"
                     /translation="MSISSPVVVVGGGIVGASIAHYLASMGQDGVVLLEKDSIGSGAT
                     GSSGALIRMHYTNPYDAALALKSLHIFENWKDTIGGDSGFRKTGFLAVADKPNAVRLQ
                     NNVEMLKDVGVNTTALSPGDIEDLQPFCNIDDLGAAAYEPDSGYADGYSTVTAMARRA
                     RDLGVSVRQGVSVFEILCENDKVIGVRTSDGDIFTPTVVLAAGAWSHHLGKTAGIQLP
                     VGPQRLTAGLVERPEELKDPHMIFIDHAVGQYFRPDSNNLTLLGIRPGPSVDLQVDPN
                     DYDASVAMEWQVRSLGQLAHRIPAMVDAGWRRSWAHVDGYPPDGHMVLGKEPDIEGMY
                     VAAGMSGSGFKTGPAVGIGMAELILEGESRTVNIDPFRINRFQENEPIISENEYQISE
                     FKFPG"
     gene            complement(3600..4496)
                     /locus_tag="QF838_02240"
     CDS             complement(3600..4496)
                     /locus_tag="QF838_02240"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HJO59544.1"
                     /translation="MRSFIESLTSGKFMVTTELISPKGTHLTPLFEKAEALRDQVDAI
                     IIPDSHAAQMSMSPLAVAHLLINRGIEPILSITCRGRNRIALQSDLLGAHALGIKNIV
                     CMTGDHPAVGDHPEATPVFDLDSISLLRAIASHRAGKDMSGHRLTGISAFSAGAVVDP
                     AITNQNQQMRRMEMKVAAGAQFFFTQAVFDPLLFKSFMRTASRFGVPIIAGLIVLKSG
                     DMARRLNAGLLGITVPDVLIDEMDRAEDPERTGTEIATRTIKDLMSLCQGVHLVSVGR
                     EYQISSVLKAAGIASDSSLRER"
     gene            complement(5031..5417)
                     /locus_tag="QF838_02245"
     CDS             complement(5031..5417)
                     /locus_tag="QF838_02245"
                     /inference="COORDINATES: protein motif:HMM:NF020950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HJO59545.1"
                     /translation="MFFIIGCSVNQGEQASFNGSCDRFLEDKHLTWNVNLQPGDSLLV
                     TLCSNTTTGFQWPESAQIKDQNILQQIGHTFTPPEKSLPGAAGKEEWTLNTIKKGASI
                     VVWEYSRPWAGGEKGEWSLTATVIVE"
     gene            complement(5771..>6872)
                     /locus_tag="QF838_02250"
     CDS             complement(5771..>6872)
                     /locus_tag="QF838_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO59546.1"
                     /translation="DPADVESAIEALKTAEQPLVIFGKGVNNADAASEMREFVERTGI
                     PYLAMPMAKGIIPDDHPQSAAAGRSFVLQNADLVVLVGARLNWMLHFGLPPRFREDVR
                     IIQLDFNPEEIGVNVATEVALIGDAKLTLRQMIDVLDRDQWQFPQDSEWLNEVGAKVR
                     ENSETVQEMMQEETSPLGYYRALRSINESLPDDSVFVAEGASTMDISRTVVNQMQPRT
                     RLDASSYGSMGLGHGFAIAAAVQTGKRVICLQGDAAFGFGGTECEVAVRHNLPITWIV
                     FNNGGIGGHDPEISSSAESGFLPQGAMNFNARYDVMMEGLGGVGYNANNSEELDDHID
                     AALKVDGPSLINVPLAPDAKRKPQKFAWLTRT"
BASE COUNT         1825 a         1662 c         1528 g         1857 t
ORIGIN      
        1 acatttatga gagaaaaatt cgacgggcag ttcgcgaaat tgttgaaaca tcgatattgg
       61 ctatgtttgt gttttctgtt ttgtatattt ctatccaaaa ttaccaggtc gaaggttcca
      121 gcatggctcc aaacataaat caaggagatt gtgtaatagc caataaagct gcctactgga
      181 agataccaga accgcgtttg gggaatttct cagaaaacaa cacttccgca ttaaaaaacc
      241 gatatttatt tggagaccca gatacaggag acgtcatagt attcacttat ccagaagatc
      301 cgaatcgcca ttttataaaa agaattattg ctacggaagg agatagcatt catatcaatg
      361 acggtgtagt tttcttaaat agtgttttat tggaggagcc atatctcgac ccagcattaa
      421 ttgatagttc caattggggt cccaatgttg tcggaatagg agaatatttc gtgcttggtg
      481 ataacagaag ggcaagcaat gactctagag attggggaac gctttcgaga aaacacataa
      541 taggaaaata cctcggacga tatccttcct cggtttgttc gatatcacct atgacacagg
      601 aaacctacga taaataacat tttttgttga agacaggaaa ttctatatat agaattgatg
      661 tttagagaaa ataaaatggg ccggtagctc agttggcaga gcatcggact tttaatccga
      721 gtgtcgtggg ttcgaatccc acccggctca ccattttgta gtcacgaaaa cagtgcattt
      781 gcgttccgtc ggggttatcc aattctcgtc ttgccgacat tttgccgaca cgggctgatc
      841 agttcgtcgc cagtaagttc acactcctga ttgaggatac ccaatcctct tctaatccct
      901 ctctatagga aacattggag gtgatttcca ccttgctaaa tcccgattct ctcattcttt
      961 ttatgtagac gtctattaac tcagctcctg caaggcagtg ggtccaattg tccatgtctg
     1021 acgacacatg gtcaggcagt tcactcgtca gcatgacgtc ggatatgtgc attctgccac
     1081 ctttcttgag aatccttaca gactccctga aaacggcgtc tttatcgggt gcgagtacta
     1141 tgacacagtt ggaaatgatt acatctaccg aatcggtatc aattcccgtt ttatcgatct
     1201 gggctaacct gaattcgacg tttcctgctt cgaccttgac cttattctcg tttgcaagat
     1261 tgatcatgtc aggtgtcatg tcgacaccga ttacacgacc ggtttcgccc accatatttg
     1321 cagcaataaa acagtcgata cctcctccgc tgccaagatc caccaccgtt tctccctgtt
     1381 tcagctctcc aatcgcgttg gggttaccgc agcccgcgga ggccataagt gcctcctccg
     1441 gcaagtcttt aatctgggta gattcataaa ctccggaacc tggtgcgaat ccgtccctct
     1501 caccgcagca atcggcagca cctctcgcaa gctcaccgta tctgtacctt acgctttcta
     1561 tgatcaactc tttatcgacc aacttcttac ctccatctgt ttctatgcat cttgatctca
     1621 acctcatctt ggcagcactc cttcagcaga aatgccgcta gcgcttcaat atgggttatt
     1681 acggcgaaat tcaataccgc acgccctctt ttttcctgcc gaataagctc ttcactttct
     1741 agaattcgca agtgatgggc cagggtagat ggtggcatac caattcgttc tcttacctca
     1801 ccaaccgtga gcccttcact ccctgctttc acgagtacaa taagcacgtt caacctcggt
     1861 tcggaaccga tagccgaaaa tccttcagct gctaatgaag tatcaaactc tagaacattg
     1921 ttgcttataa ccatacaact agtattctag gtttagggat ctgtgtcaac aacctctcca
     1981 ttgttttaag attagcgatt catacttgtc cacagtcggc actagcctga cagaaagggg
     2041 ctggaagccg tacgatacga ggcatccacc agatctaaag gccccctgtc gactggcaga
     2101 gggtccgtct acactttgca ctagggtcaa ggtttgtgaa atttatttga atacacaaat
     2161 gatttccaga agtaaatgga gtttaagccc tattcttgac cccggtctct tattttatgc
     2221 aaggttgtat agcgaatgca tattgaataa aagttcatta tggtgtctga tgcaatggaa
     2281 catttcgtga agagctgtag atttggtaaa tacctcgctc taaacgctgt ccaacaaatt
     2341 gttaggaggt ttggaatgtc gatttcttcg ccagtagtag tcgtaggagg tggcatcgtc
     2401 ggggccagca tagctcacta cctcgcaagt atgggtcaag atggagtggt cttgcttgag
     2461 aaagatagta ttggcagtgg tgccacaggt agttcaggtg ctctgatccg aatgcactac
     2521 accaatccgt acgacgcggc actcgccttg aaaagccttc atatttttga gaactggaag
     2581 gataccattg gtggagattc gggatttcgg aaaacgggtt ttctggcagt tgcagacaag
     2641 cccaacgcag taagacttca aaacaatgtt gaaatgttaa aagatgtagg agtcaatacc
     2701 accgcactct cccctgggga tattgaggat ctccagccgt tttgcaatat agatgacctc
     2761 ggtgcggcgg cttacgagcc ggatagcggg tatgccgatg gatactctac agttacggca
     2821 atggccagaa gggcgcgtga tctgggtgtc tccgttcggc aaggagtttc ggtattcgaa
     2881 atcctttgtg agaacgataa agttatagga gtaagaacgt ccgatgggga tatcttcacc
     2941 ccgacggtag tcttggctgc tggcgcctgg tctcatcact tgggtaagac cgctggaatt
     3001 caactacccg tgggaccgca gcgattaacc gcaggccttg tcgaacggcc tgaggagttg
     3061 aaggatcctc acatgatctt tatcgatcat gccgtaggcc aatattttag gcctgactcg
     3121 aataatctga cattgctggg catacgaccg gggccctcgg ttgatctgca agtagatcca
     3181 aacgactatg atgccagtgt agctatggaa tggcaggtcc gttcgcttgg tcagctcgca
     3241 cacaggattc ccgctatggt cgatgctggc tggcgtagat cttgggcgca tgttgatggc
     3301 tacccaccgg atggccatat ggtcctagga aaagagccgg atattgaagg aatgtatgtt
     3361 gctgctggga tgagtggaag tggattcaaa accggtcccg cagttggaat aggtatggca
     3421 gaacttattt tggagggcga gagtcgaact gtaaatattg atccatttcg aattaatcgc
     3481 tttcaggaaa acgagcctat tattagtgag aatgaatacc aaatatctga attcaaattt
     3541 cctggttaat atgcagttac caacagcaat caccgatcct aacctgtttg caaagttatc
     3601 tacctctctc ttagagatga gtcactagca ataccagcag ccttcagcac gctggatatt
     3661 tgatattctc tcccgactga cactaaatgc acaccctgac acaaggacat tagatctttg
     3721 atggttcgcg tcgctatctc ggtgccagtt ctttcaggat cctctgcccg atccatctcg
     3781 tcgatcagga catctggaac agtgattcca agtagcccgg cgttcaggcg acgggccatg
     3841 tcaccggact tgagcacaat caatcccgcg atgataggaa caccgaacct gcttgcggtg
     3901 cgcatgaatg acttgaaaag gagcggatca aacactgcct gggtaaaaaa aaattgggcg
     3961 ccggcagcca ccttcatctc cattcgtcgc atttgctgat tctggtttgt aatcgccgga
     4021 tcgaccactg caccggctga gaaagcagag attcccgtga gacggtgacc tgacatatcc
     4081 tttccagccc ggtgagaagc aatggccctt agcagagaaa tggaatcgag gtcgaaaacg
     4141 ggagtggcct ccgggtggtc accgacagcc gggtggtcgc cggtcatgca aacgatattt
     4201 ttgataccca aagcatgcgc ccccaagagg tctgactgta gggctattcg gttgcgcccc
     4261 ctacaagtga tactgagaat aggctctata cctcggttga ttagcaaatg agccaccgct
     4321 agtggagaca tactcatctg ggcggcatgg gaatccggga tgatgattgc gtctacctga
     4381 tccctcaacg cttccgcttt ctcaaataga ggtgtgaggt gagtcccttt tggtgaaatc
     4441 agctcggtgg taaccatgaa tttccccgac gtaagggatt cgatgaacga tctcactatt
     4501 acctccgtat gtgtaaattt gtttttcgat gccttttagt accaatggcc ttctgtacaa
     4561 aatcagtatt actatttgta gtaggtttat taacactccg gcaaaattcc aacttagact
     4621 tgtccacact atttcatctc taaatatgta cagagggaag acaagtaccg ttcccaaaac
     4681 agcaaaaaca cgcagccata aaatatccct aaaagtcaat gatattaaga tgatagcgaa
     4741 gcctaaattt gctagtaaaa gaggtatatt tggcatattt atgtaagact aaaatccatt
     4801 aaataccctt taatctgaat cttcagtttc cggcacccct cactaggtat ttcataagtg
     4861 ctcctgatgg aatcaaattt ctcggaatgt attgtgccat gagactagga taacgcttat
     4921 ggtgtccaca ccaacatatc atttctgaac tcatgccatt cttccaccgc cattataggc
     4981 atcttggtat tatgtgcgag ttatcgtgaa agtcacactt tgttgcgagc ctattcaaca
     5041 atgacagttg cagttaatga ccactctccc ttttcaccac cagcccatgg acggctatat
     5101 tcccacacga cgatactggc cccttttttg atcgtattaa gagtccattc ttctttgcca
     5161 gcggctccag ggagactctt ctcaggaggt gtaaaggtgt ggcctatctg ttgcagaatg
     5221 ttctggtcct ttatctgtgc ggactcaggc cattggaagc ctgtggtagt gttggaacaa
     5281 agggttacaa gcagtgagtc acccggttgt aaattaacat tccacgttaa gtgcttgtct
     5341 tccaagaagc ggtcgcaact tccgttgaag gaggcttgtt cgccctgatt aaccgagcat
     5401 cctatgataa aaaacattac caacaacacg tacttcatgg caccattata agtaattcaa
     5461 aaatgtgtgt gggggtatgc tcgctaaata gagataggta gggacgaagc tcctactagg
     5521 gctagtacca ttaactggtc atctcagccc cagtggaggg actgagatga ctctaatcgg
     5581 agtacagatt tttactcctc cattattagt ctgtaactgg ctaagttttc attccattca
     5641 aagacattta aatattcaaa tcctataaca ccaaaaaaat gggccaattg ctgccaggac
     5701 aataaggtac aaatacgatt cgaatctgtt gaggtcgata ttcggaaaaa aactaccctc
     5761 acttctaacc ttatgttcga gtcaaccaag cgaatttctg gggctttcgc tttgcgtcag
     5821 gagccaacgg aacattaatc agagatggcc catccacctt taacgctgcg tcgatatgat
     5881 catctaactc ttctgagttg ttagcattgt aaccgacccc tccaagacct tccatcatca
     5941 cgtcataacg agcgttaaag ttcatggctc cttgtggaag aaaccctgat tcagcgctcg
     6001 aagaaatctc tggatcatgc cccccgattc cgccattgtt gaatacaatc cacgtaatag
     6061 gaaggttatg tcgcaccgcg acctcacact ctgttccgcc aaaaccgaaa gccgcatcac
     6121 cttggagaca aattactcgc ttacctgtct gcaccgcagc tgcaatagcg aacccatgtc
     6181 ctaaccccat agatccatac gaagacgcat ctaaacgcgt tcgtggctgc atctggttga
     6241 caacagtacg tgaaatgtcc atcgtgctgg caccttcagc tacaaagacc gaatcatctg
     6301 gcaaactctc gttgatggaa cgtagcgccc gataatatcc aagaggtgag gtctcctcct
     6361 gcatcatctc ctggacagtc tcagaatttt cccgcacctt ggcacccact tcattcaacc
     6421 attctgaatc ctgtgggaat tgccattgat ctctgtccaa tacatcaatc atctgacgta
     6481 gagtgagttt ggcgtcacca atcaatgcaa cctcagtcgc cacatttact ccgatttcct
     6541 ctgggttaaa atctaactga attattctca catcttctct gaatcgtgga ggaagtccga
     6601 agtgcaacat ccaattcagt cgagctccta ctaacacaac cagatcggca ttttgaagca
     6661 cgaatgaacg accggcagcc gcggattgtg ggtgatcatc agggataata cctttcgcca
     6721 taggcatggc gagataaggt atcccagttc tttcaacaaa ctctctcatc tcgctggccg
     6781 catcagcgtt attcacgccc ttgccgaaaa taaccagtgg ctgctcagcg gtttttaaag
     6841 cctcgattgc ggactcaaca tcagccggat cc
//
LOCUS       DASCNS010000055         3386 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000460930, whole genome shotgun sequence.
ACCESSION   DASCNS010000055 DASCNS010000000
VERSION     DASCNS010000055.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000460930"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            101..949
                     /locus_tag="QF838_02255"
     CDS             101..949
                     /locus_tag="QF838_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJO59547.1"
                     /translation="MASRIIENKVTAYLGHEDSPDISNPIHSSEVAKAYGFPGALVGG
                     VTVWGWATDTILEALGEDWLKVGWSEFSFRQPTIPDDEMLIRATADNGSGSWSVEMIN
                     QDDVICVVGKVGLGKSEWSSEFVRSTSLNPTGDEQPKKSLTLETAEIGSDWAGMKINF
                     TEALANEFTTLKQQTNNPLFVGANPIAHPSWTAGWAEQLMRHNYDIPSSMHTRSRIQH
                     LNVVPTGTQVFGGAHMVAAYERKAHHFVNFDVLLQDQNGIDIAQLRHWTIFKIATPEE
                     RENAIS"
     gene            complement(946..1761)
                     /locus_tag="QF838_02260"
     CDS             complement(946..1761)
                     /locus_tag="QF838_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJO59548.1"
                     /translation="MTDMVQVHDREHVRVIKMNRPAKKNAASLELAWGVIEAIRDAAG
                     EENVWVIGLTGVGDAFCAGLDLTPGEDQEKFIPMSDQDRYLDDLGWVSRFYLTLREEC
                     DKPIVGGINGVAVGAGLALAMATDIRIMATSARLLAGYPRIGGSPDGGLSMTLSQAIG
                     YEQAMKFLLENRTVTGKEAMEIGIVSRVVSDELFEEEFNHYLDSLTSLSPITARTTKR
                     VLRAANRIDIEKQLHYELLNIGKSLGSLDGQEARKAFIEKRPPIFTGKTEQRT"
     gene            complement(1829..2575)
                     /locus_tag="QF838_02265"
     CDS             complement(1829..2575)
                     /locus_tag="QF838_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59549.1"
                     /translation="MDLQFELGKPDAPKGHALIYFNEVSTGSCFASYIVLLPITVDVS
                     KYVPPFLMNQVGEIGPGDMSAFAFPPAPEEVEDSEFLKNLAETRHDDLIFGGDYTASD
                     VTSAMMKVNDVVQAYLELYEEGSGVGIHEQNTAISEKSNFQVNDIIYSMMSESDRLSE
                     LTKLVGRMRYAIESDEESLVVETEADLNAISTYFSEIFQMPILIEWACKEGKRASGIA
                     DLYLKRCYHLSLEEYEDLGRVEEQIKSLGD"
     gene            2722..>3386
                     /locus_tag="QF838_02270"
     CDS             2722..>3386
                     /locus_tag="QF838_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59550.1"
                     /translation="MQLYKKLMRPLLFTLQPEMAWHLSDLGLSQTWFWQMIGNNFQHH
                     NELLEATVAGIPIKNPVGLAAGYDKNCKFLSSLQELGFGYVMGGTITKDARPGNPKPR
                     VIRLKNQSALINALGFPGDGLERALSEISKMVSIPKKSVRIMSVSGTDIPDITECHRQ
                     LEPFCDAMELNISSPNTEGLRIFQDPTKLRSLLAEVNQSRTKPLLVKMPPYKSDEGDH
                     EKTL"
BASE COUNT          995 a          771 c          681 g          939 t
ORIGIN      
        1 attcaacatt gtgagatact gactaaataa aggacacagc cgatatatgt aaagtatcct
       61 acaaacccaa actgaaccag ttaaatcaag aggtgaagga atggcatcga gaatcattga
      121 aaacaaagtc actgcttacc tgggacatga agatagcccg gatatttcta atcccattca
      181 ttcctcagaa gtcgcaaagg catatggttt ccctggagcc cttgtcgggg gggtgactgt
      241 ttggggttgg gctactgaca ctatcctcga ggctttgggg gaagattggt tgaaggttgg
      301 ctggtcagaa ttttctttcc ggcaacctac aataccggat gatgaaatgc tcattagggc
      361 aactgcagat aatgggtcag gatcctggtc tgtggaaatg attaaccaag atgatgttat
      421 atgtgtggtt gggaaagtcg gtttgggaaa atcagagtgg tccagtgagt ttgttcgctc
      481 tacatcctta aatccaactg gagatgagca accaaaaaaa tctttgacac tggaaactgc
      541 cgaaatcgga tccgactggg ctggaatgaa aataaatttc acggaggcgc ttgcaaacga
      601 attcaccaca ttaaagcaac agacaaacaa ccctttgttt gtgggtgcta acccaatcgc
      661 tcacccatcg tggactgcag gatgggccga acagcttatg agacataatt acgatattcc
      721 gagttcaatg cataccagaa gtagaataca acatcttaat gttgtaccta ccggaactca
      781 ggtattcggc ggtgcccata tggttgctgc atatgagaga aaggcccatc attttgttaa
      841 ctttgatgtg cttttacagg atcaaaatgg gatagacata gctcaattaa gacattggac
      901 tatatttaaa atcgctaccc ctgaagaaag agaaaacgca atcagttagg ttctttgttc
      961 agttttccca gtgaaaatag ggggacgttt ttctataaat gcttttctag cttcctggcc
     1021 gtccaaacta cccaatgatt tgcctatgtt gagtagttca tagtgaagtt gtttttcaat
     1081 atcgatccga tttgccgccc tgaggactct ttttgtggtc cttgctgtga tcggagataa
     1141 gctagtcagg gagtctaggt aatgattgaa ctcctcctca aataattcat cagaaacaac
     1201 tcggctaacg atgcctattt ccattgcttc cttacctgta acggttctat tttcaaggag
     1261 aaatttcata gcttgctcat agcctatcgc ttgggacaag gtcatcgata ggccaccgtc
     1321 gggggacccg ccaattcgag gataccctgc caagagacga gcggaggtag ccattattcg
     1381 gatatccgtc gccatagcta aggccaaccc tgccccaacc gcaacgccat taatcccacc
     1441 aacaatcggt ttgtcgcact cttctcttaa agtcaggtag aaacggctaa cccaaccaag
     1501 atcatctaga tatctatcct ggtctgacat tggtatgaat ttttcttgat cttcccctgg
     1561 agtcaggtcc aatccagcgc aaaaagcgtc tcccacaccg gtcagcccga ttacccaaac
     1621 gttttcttcc cctgcagcat ctctaattgc ttctattacg ccccaagcga gttctaaaga
     1681 ggccgcattc ttttttgccg gcctgttcat tttgatcacc cgaacatgct ctctgtcatg
     1741 aacttgaacc atatcagtca ttgaaacgtc tccgttgaag ttatttaaca gtggatttta
     1801 aaccaacgat catactcaaa agtggacact agtcacctaa ggattttatt tgttcttcca
     1861 ctcttcctaa atcttcgtat tcttctaaac ttaggtggta acaccttttc agatataaat
     1921 ctgcgatacc agaggccctt ttcccttctt tacaggccca ttcaatcaaa ataggcatct
     1981 gaaagatttc cgagaaataa gtggagatag catttagatc cgcctctgtt tcaacaacaa
     2041 gggattcttc gtcggattca atagcatatc gcattcgccc aacaagtttc gttaattcgc
     2101 tcaaccggtc agattcactc atcatagagt aaataatatc gtttacttgg aaatttgatt
     2161 tttcagaaat tgcagtgttc tgctcatgaa tacctacacc agaaccctct tcatatagtt
     2221 ctaaataagc ctgcaccaca tcattcacct tcatcatagc ggatgttaca tcggaggctg
     2281 tgtagtctcc accaaatatc aaatcatcgt gtctcgtttc tgccaggttt ttcaaaaact
     2341 ctgaatcctc cacctcttca ggagcaggag ggaatgcaaa ggccgacatg tcgccaggcc
     2401 cgatttcacc tacttgattc atcaaaaatg gcggaacgta cttacttaca tcgacagtga
     2461 tggggagaag gacgatatag gaggcaaaac aagatccagt ggaaacttca ttgaaataaa
     2521 tcaatgcgtg accttttgga gcatcaggct ttccaagctc aaattgcaaa tccatatgta
     2581 ttaaagattc cctttatcta gctattgtcc attcgccgcc accctgacca ttatatgcca
     2641 gatcatagac aacccatata actacactcg tcataatttc aatataatag ggtctgtaac
     2701 tgataggttt aggggcatca tatgcagttg tataagaaat taatgagacc gcttctattc
     2761 acattgcagc cagaaatggc ttggcacctg tcggatcttg gcctctccca aacctggttt
     2821 tggcaaatga ttggaaacaa ttttcaacac cacaacgaac ttttagaagc tacagtggcg
     2881 ggtatcccaa taaaaaaccc agttggtttg gctgcaggct atgataaaaa ttgcaaattt
     2941 ttatcatctc ttcaagagct tggtttcgga tatgtcatgg gtggcacaat caccaaagat
     3001 gcaagaccgg gtaatcctaa accgagagtc attagactga aaaaccaatc agctttaatc
     3061 aatgctttag gcttccctgg agatggtcta gaaagagcct tatccgaaat atccaagatg
     3121 gtatctattc ctaaaaagtc ggtacgcatc atgagtgttt ctggaacaga tattcctgac
     3181 attactgaat gccaccgaca acttgaaccc ttttgtgatg ccatggaatt aaatataagc
     3241 tcacctaaca ctgagggcct cagaattttc caagacccga caaaacttag atcactgtta
     3301 gctgaagtaa accaaagtag aactaaacct ctccttgtaa aaatgcctcc atataagtcc
     3361 gatgaaggtg atcatgaaaa aaccct
//
LOCUS       DASCNS010000056         4508 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000463836, whole genome shotgun sequence.
ACCESSION   DASCNS010000056 DASCNS010000000
VERSION     DASCNS010000056.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000463836"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="cysS"
                     /locus_tag="QF838_02275"
     CDS             complement(<1..847)
                     /gene="cysS"
                     /locus_tag="QF838_02275"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HJO59551.1"
                     /translation="MRLYNTLNRKVEELVVEDNTVNMYVCGITPYAPSHLGHAMCAIV
                     FDVIRRYLEYKGYKVHFIQNFTDIDDKMITAANEEGIEVSALAERNIQAYLSELGQLN
                     VLQASEYPRATEEISSIIAVITDLIEKNYAYAISGDVYFRVKEDEDYGKLSRRNVDEL
                     LAGARLEIEETKENPADFALWKSQKPGEPAWDSPWGQGRPGWHIECTAMSLTYLNNRV
                     DIHGGGQDLVFPHHENEIAQSESYTGVEPFARYWMHNGTLGYGQEKMSKSLGNVFSIA
                     EALEEF"
     gene            complement(844..1329)
                     /gene="ispF"
                     /locus_tag="QF838_02280"
     CDS             complement(844..1329)
                     /gene="ispF"
                     /locus_tag="QF838_02280"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HJO59552.1"
                     /translation="MNIIRTGIGVDIHRLEKGESLILGGVEIPSQYGLLGHSDGDVLI
                     HSIIDGLLGAANLGDIGRYLPHTKAEYKGISSQKLLEIALSRVVEEGWKPVFVDATIV
                     AEKPLVAEFIPQMTFNIAHAINLAPKDISIKATTADGLGYIGRGEGISCQAIVTIQKP
                     E"
     gene            complement(1326..2000)
                     /gene="ispD"
                     /locus_tag="QF838_02285"
     CDS             complement(1326..2000)
                     /gene="ispD"
                     /locus_tag="QF838_02285"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HJO59553.1"
                     /translation="MNGFSAIILGAGTGNRMRGIDKILTTICGLPVILHSVDEFLRSG
                     IFETMTVVANANNISKLEHLMSGPQYRTVQVILGGIRRQDSVKNALATTNTADYVMIH
                     DGARPCVSIDLIERAAKATQTSDAAIPVLPVTDTIKRVKDFKVVSTVDRSNLYLTQTP
                     QVFKYSSIAKFHKMFGANVTDDSLLVEMSETDVSTFEGDPENIKITNPIDIEIAETIL
                     QRRKNT"
     gene            2193..2654
                     /locus_tag="QF838_02290"
     CDS             2193..2654
                     /locus_tag="QF838_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59554.1"
                     /translation="MGSNYWMFVDNSENSAITRKKGYKVFGMSAKYKRRAQRMHAKDR
                     VIFFDRNRKCWTASATIISDYFEDESPIWVPIDKPREYKFRISLKPDYVLEEEQYIDG
                     LQLGPSLEYVKRWAPEDWPLAFWDRIHLLPSRDFKLIEDEMKRAKKQKQAN"
     gene            complement(2672..3652)
                     /locus_tag="QF838_02295"
     CDS             complement(2672..3652)
                     /locus_tag="QF838_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO59555.1"
                     /translation="MKYSDFSVNDINLSVIGFPLWTLATKGWGKKDPSIAIYLLQQAF
                     NQGINFFDTADSYGKGYGEELIREALSPIRKEIVISTKFGFDFYSPQLNVDGGDGKNF
                     DPEYVSYACEQSLRRLGTDYIDMYLVHYPSYTEVENDDLYEVLERLVDTGKILRFGLA
                     IDDRPEATEIASLLLSERELGLIHAPYSLLEQDLVSSLSEIGGDLTVIARRVHAFGLL
                     DDSYDPKMLEGSNLAQQEGHPVFKNVLARRPYYDFLSDSFEENISDIALRFAITNRDI
                     ASVLPNINDIGDLSDYSLSADKSDLDDGLMQMICDVFQDQKESEQKEEYE"
     gene            3792..>4508
                     /gene="selD"
                     /locus_tag="QF838_02300"
     CDS             3792..>4508
                     /gene="selD"
                     /locus_tag="QF838_02300"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3846..3848,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HJO59556.1"
                     /translation="MQHQHEKVRLTELASCAGUASKLSPDDLAQVLSQLPDMTHPNLL
                     VGTKTSDDAAVYKLSDDLAVVLTIDFFPPIVDDPFTFGQIAAANSLSDVYAMGAKPIA
                     ALNIVGFPSDLDMSILAEILKGGASKALEAGIIIAGGHSVVDAEPKYGLSVTGIVNPG
                     SQTANSNSKTGDLLVLTKPIGTGVITTAGKQEKVDDGVLKNAVEIMATLNKTASEAMI
                     SVGVNACSDVTGFGLLGHLRE"
BASE COUNT         1368 a          978 c          846 g         1316 t
ORIGIN      
        1 agaattcttc taaggcttct gcaatggaaa atacattccc taaagatttg ctcatttttt
       61 cttgaccata gcctaaagta ccattatgca tccaatatcg ggcaaaaggt tcaacgcctg
      121 tgtatgactc agattgagca atttcatttt cgtgatgtgg gaacaccaaa tcttgaccac
      181 caccatgaat atcaactcta ttattaaggt acgttaacga catggcagta cattctatgt
      241 gccatcctgg acgtccctgc ccccaagggc tatcccaagc tggttcccct ggtttttgac
      301 ttttccatag agcgaaatct gcagggtttt ccttagtctc ttctatttcc aatctagcgc
      361 cagctaacag ttcatcaaca ttcctcctgc ttaacttccc atagtcttca tcttccttta
      421 ccctgaaata aacgtcacca gaaattgcat atgcatagtt tttctcaatc aagtcagtaa
      481 tgacggctat aatggaactg atctcttcag tagctcttgg gtattctgaa gcctgaagca
      541 cattcagttg tcccaattct gagaggtaag cctgaatatt tcgttctgcc aatgctgaca
      601 cttcaatccc ttcctcattg gcggcggtta tcatcttgtc gtctatatca gtaaagtttt
      661 gtatgaaatg aactttatat cctttgtatt ccagatatcg tctaatgaca tcaaaaacta
      721 tcgcacacat ggcatggccc aaatgagaag gtgcataggg agttataccg caaacataca
      781 tgttaaccgt attgtcttct acaacaagtt cttctacttt cctattcagt gtgttgtaca
      841 gtctcattct ggtttttgga tagtaactat agcctggcag gaaataccct ctcccctacc
      901 gatatatccc aacccatcag ccgtagttgc tttaatactg atgtcttttg gagctaagtt
      961 aatagcatgc gctatgttaa atgtcatttg tggtataaat tccgcgacca gtggtttttc
     1021 ggcaacgatt gtagcatcca caaacacagg cttccatcct tcctctacaa ctctacttaa
     1081 agcaatttct agtaactttt ggctacttat ccctttatat tctgctttag tatgaggtaa
     1141 atacctaccg atatcaccta aatttgccgc acctaaaagc ccatctatga ttgaatgtat
     1201 caggacgtca ccatcactat gccccagcaa accatattga gatgggattt ccaccccccc
     1261 caaaatcaag gactcgcctt tttctaacct gtgtatatca actccaatac cagtgcgaat
     1321 aatattcatg tatttttgcg tctctgtaaa atagtttctg caatttcaat atctattgga
     1381 ttcgttattt ttatattctc aggatctccc tcaaaggtag aaacgtcagt ttcagacatt
     1441 tctaccagca aggagtcatc tgtaacatta gccccaaaca ttttatgaaa tttagctatg
     1501 ctggaatatt tgaagacctg aggtgtttgg gtcaaataaa gattggatct atctacagtg
     1561 gacacaacct taaagtcttt tactctcttt atagtatctg taactggtag cacaggtatc
     1621 gccgcatctg aggtttgagt cgccttagca gcgcgttcta taagatcaat agatacacaa
     1681 ggcctagcac catcatgaat catcacatag tcggcagtat tggtagtagc tagagcattt
     1741 ttaacagaat cttgtctccg aatgcctcct agaatcacct gaaccgtgcg atattggggg
     1801 ccagacatca aatgttccag cttggaaata ttgttcgcgt tagccaccac tgtcattgtt
     1861 tcaaagattc ccgatctaag aaattcatct acagaatgca atatcaccgg cagcccacaa
     1921 atagtggtca gtattttatc gatgccacgc attctgtttc cagtgcccgc acctaaaatt
     1981 atcgctgaaa aaccgttcat agaaattagc cgatctgact taccgagata ttaataatca
     2041 tcacaataca agagtcattt tatacgttca tcctacaagc tcgcatcctc tataaagaga
     2101 cccttcaaaa cagatgtaca ataatgtgaa aaacactccc tctgccaaaa ctaaacatag
     2161 taatctcaga gtagagaaaa taggggaaaa agatgggctc caattattgg atgtttgtgg
     2221 acaacagcga aaattctgct attacacgga agaaaggcta caaggtattt ggcatgagcg
     2281 ctaaatataa aagacgggcc caaagaatgc atgcaaaaga tcgagtgatc ttttttgaca
     2341 ggaatagaaa atgttggacc gcatccgcca ccataatctc cgactatttt gaggatgagt
     2401 ctccaatttg ggttcctata gataaaccca gggaatataa attcaggatc tctttgaaac
     2461 ctgactatgt tttagaggaa gaacaataca tagacggcct tcaattaggg ccaagtttag
     2521 agtacgtgaa gagatgggct cccgaagatt ggcccctagc tttttgggat cgaatacatt
     2581 tactacccag tagagacttc aagttgattg aggacgaaat gaaaagagct aaaaagcaaa
     2641 aacaggccaa ctaacatggg ctcgttagct tttattcata ttcctctttt tgctcacttt
     2701 ctttctggtc ttgaaataca tcacatatca tttgcattag tccgtcatcc aaatctgatt
     2761 tatcggccga caaagagtaa tccgacaagt ctccaatatc attgatgttt ggaagtaccg
     2821 aagcaatatc tcgatttgtt atcgcgaatc tcaaggcgat atctgaaatg ttttcctcaa
     2881 aactatctga aaggaaatcg taatatggtc tccttgctaa gacatttttg aatacaggat
     2941 gaccttcttg ttgagcaagg ttagaacctt cgagcatttt ggggtcgtaa gaatcatcta
     3001 gcagaccgaa agcatgtact cttctggcta tcacagttaa gtcgccgcct atttccgaaa
     3061 gacttgaaac taagtcctgc tccagcaaac tatatggggc atgaattaaa cctaactctc
     3121 tttccgaaag gagcagacta gctatttctg tagcttcagg gcgatcatcg attgctaggc
     3181 caaacctcaa aatcttgcct gtgtctacaa gtctttcgag cacctcgtat aaatcgtcat
     3241 tctcgacctc agtataagac ggataatgga caagatacat atcaatgtaa tcagtcccaa
     3301 ggcggcgtaa agactgttca caggcataag aaacatattc ggggtcaaag tttttgccgt
     3361 ctccaccatc aacgttcaat tgaggtgaat aaaagtcgaa cccaaatttt gtggatataa
     3421 caatttcttt ccttattgga gagagtgctt ctcgaattaa ttcttccccg taccccttac
     3481 cataagaatc tgcagtatca aaaaaattta tgccttgatt aaacgcttgc tgcaataaat
     3541 aaatggcaat gctaggatct tttttccccc agcctttggt cgccaaagtc cacaagggaa
     3601 agcctatgac agacaaatta atatcattaa ctgaaaaatc tgaatatttc atctacaaaa
     3661 tgaaaacctt tttacttact caaaaataat ggtattaaat gatatccgat agtgaatata
     3721 cattttatgc ttcaaagcaa cccggataca gatataatac tcagtaattt gcatcagtca
     3781 ggatattaaa tatgcagcat caacatgaaa aagttaggct cacagaactt gcatcatgtg
     3841 caggttgagc gtctaaacta agtccagacg acttggctca ggtcctgagt caactaccag
     3901 atatgaccca tccaaaccta ttggttggaa caaagactag tgatgatgcc gccgtctaca
     3961 aattaagcga tgatttggct gtcgttttaa caatcgattt ttttcctccg attgtggatg
     4021 atccatttac atttgggcaa atagctgctg ctaactcact aagtgacgtc tacgcaatgg
     4081 gagctaaacc gatagcggcc ctaaatatag tcgggtttcc ttcagatttg gacatgtcta
     4141 tcttggccga aatcttaaaa ggcggagctt caaaagcact ggaagcggga atcattatag
     4201 ctggggggca tagcgtagtg gatgccgaac ccaaatatgg actttcagta acaggaatag
     4261 ttaatccagg atctcaaaca gcaaatagta attcgaaaac tggagactta ttggtattaa
     4321 cgaagccaat aggcaccggc gtgattacca ccgcggggaa acaagaaaaa gtagatgacg
     4381 gtgtactaaa aaatgccgta gaaattatgg caacactcaa taaaacggct tctgaggcta
     4441 tgatttcagt cggggtgaat gcttgttccg acgttacagg atttggttta ttaggacatt
     4501 taagagag
//
LOCUS       DASCNS010000057         3551 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000474471, whole genome shotgun sequence.
ACCESSION   DASCNS010000057 DASCNS010000000
VERSION     DASCNS010000057.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000474471"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="QF838_02305"
     CDS             <1..428
                     /locus_tag="QF838_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HJO59557.1"
                     /translation="NSSGEVIGINTAIIDGSNGIGFAINIDDSHVVVDQILAFGNVLR
                     GFLGITPFNVTDSIREQINLPVSDGVIIAGVVSGFPAEESGLQPEDVIVMLNKTSIEN
                     SGDLSKFLLNNPPGSEVEVRFYRRGKLKNVELVLADKPD"
     gene            585..1052
                     /locus_tag="QF838_02310"
     CDS             585..1052
                     /locus_tag="QF838_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HJO59558.1"
                     /translation="MLFLFLSTACFAANSDFISQEEKKINRLNGVLMCPVCPGESIDQ
                     SQNEIASNMRTIVKGLVEEGKSEDEIKAYFVGRYGPVILLEPATSGINIYAWIIPPVA
                     LGFAIIVVIVALGVMRRKEKSTGSDQPYNLKAEAISIEEKEKYFPVIDRISNE"
     gene            1089..3089
                     /locus_tag="QF838_02315"
     CDS             1089..3089
                     /locus_tag="QF838_02315"
                     /inference="COORDINATES: protein motif:HMM:NF027651.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HJO59559.1"
                     /translation="MVELGQIALILSFMMAGYSTIVGFAGGMLNGRDLAASARWGFYS
                     ISPLLLLATAALIYAFVTNDFSVKYVAENSNIAMPKAYAWVAFYSGNAGSLLYISLAF
                     SIMAGIAIRVVNSKLPYTSPYALGVMSMVLLFFLAVILFLANPLDQHQVPLEDGQGMN
                     PLLIHFGMFIHPPFQMLGLISVALPFSIAIGALLAGRGGRDEWVDQGRLWGMVSWLLL
                     TTGLLLGAWWAYTILGWGGYWAWDPVENSALMPWLAMTAFVHSIMVQKRRGMFRMWNM
                     VLIIVSFAMAQMGMFINRGGPVPSVHSFAESTMGWIFLAVMAATLFISLSIFVFRYDT
                     LKSRSGLDSIISRESAFLAQNVLFLLIAFVTLWGTIFPIFSEAVDDTTMTIGQPFFNK
                     VNGPLMLLLILLMGIGPVLPWRKAGWTSINKMLRLPFVVASLVLVSSLISGITQYWVL
                     ISFVACSVAITTVCQELIRGTRSRHSHGENWVLSFFSLIGANRPRYGGYIVHLSIVML
                     AIGITASSFYDVQKDVVMKPGDSESLGKYTFKYIDVSEKTYSDRMEETAKFEVWSADN
                     YIGYMYPYRAVYPEFQIAATRGAIHSTILEDFYLVPSEFREDGQAVFRVLINPLVLWM
                     WAAGPVLILGTLISLSPNNRSHRKRVSLPSIDVDQNPFGMAK"
     gene            3090..3467
                     /locus_tag="QF838_02320"
     CDS             3090..3467
                     /locus_tag="QF838_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59560.1"
                     /translation="MLVVSAIIISVIPAILIAYPVLKKIASRMPLPEDESSLRADLER
                     RWESALEGIRHAELEMSIGNVSESDYLWLKQVHMAEAANVLKTLEISAEKERLLIEEI
                     SSAANTRSLDSSFENGHEKEDWN"
BASE COUNT          953 a          618 c          849 g         1131 t
ORIGIN      
        1 ccaatagcag tggagaagtc ataggaatca acacggcaat cattgatggg agtaatggaa
       61 ttgggtttgc cataaatatt gatgattcgc atgtggtggt tgatcaaata ttggcttttg
      121 ggaatgtact ccgaggattt ttaggaatta ctccctttaa tgtaaccgat tcaataagag
      181 aacagataaa cttgcccgtt tcagatggtg tgattattgc cggagtagtg agcgggtttc
      241 cagctgagga gtcgggttta caacctgagg atgtgattgt catgttgaat aaaacctcta
      301 tcgagaattc cggagatctt tccaaattcc tattgaataa tcctcctggt tcggaggtag
      361 aggtgcggtt ttatagacgc ggaaaactaa agaacgtgga gttggtttta gcggataagc
      421 cagattaaat aatttgagcc aaatgtgatg gattatttac ccttaatcgt tttagggact
      481 attgttccgc ataatattgg gcgataattg tagaaaagtt tttattgtga aatctccaca
      541 gcttgcgaaa aaatatgcgc cttagtttga taagtcccct gtgtttgtta tttttatttc
      601 ttagtactgc ttgcttcgcc gcaaactctg atttcatctc tcaggaagaa aaaaagataa
      661 atcgccttaa tggtgttctt atgtgcccag tttgccctgg agaatctata gatcaatcac
      721 aaaatgaaat cgcgtcaaat atgagaacga tcgtgaaagg tttagtagag gaaggaaaat
      781 cagaagatga aataaaggct tattttgttg ggagatatgg tcctgtcatt cttctagaac
      841 cagccacatc ggggataaat atctatgctt ggattattcc tccggtggcc ctgggttttg
      901 caattatcgt tgtaatagtt gccttgggcg tgatgaggag aaaagaaaaa tctactggct
      961 cagaccagcc atataatttg aaggcagagg ctatttctat tgaggaaaag gaaaaatatt
     1021 tccccgtgat agaccgaata tctaatgaat gatttacagc taaaattcca cacagtggtg
     1081 tttcttagat ggtagaacta ggacaaattg cgcttattct ttcttttatg atggcaggat
     1141 attccacgat tgtggggttt gctggtggga tgctgaatgg tagggatctt gcagcaagtg
     1201 cacggtgggg attttattcc atatctccgt tgcttctatt agctacagca gccctaattt
     1261 atgcctttgt tacaaatgat ttttctgtta aatatgttgc ggaaaacagc aatatagcaa
     1321 tgcctaaagc ttatgcatgg gttgctttct actctggaaa tgctgggtcc cttttataca
     1381 tatcactggc attttcaata atggctggta ttgccattag agttgttaat agtaagttgc
     1441 catacacatc tccttatgcg ttgggtgtaa tgagtatggt cttgctgttt tttctagcag
     1501 ttattttgtt tttggcgaac cctttagatc aacatcaggt tcctcttgaa gatggacaag
     1561 gaatgaatcc cttgttaatt cattttggca tgtttataca cccacctttt caaatgctgg
     1621 gtcttatttc ggttgcttta ccattttcta tcgcgattgg agctttactg gcaggaaggg
     1681 gaggccgaga tgaatgggtt gaccaaggca ggctttgggg aatggtgtcg tggctattac
     1741 tcacaacggg actattactg ggtgcgtggt gggcatatac catattaggt tggggtggat
     1801 attgggcatg ggatcccgta gaaaattcag ctttgatgcc gtggttagca atgaccgctt
     1861 ttgtccattc aataatggtt cagaagcggc gtggaatgtt tcgcatgtgg aatatggtgc
     1921 taattatcgt aagttttgca atggctcaga tgggcatgtt tattaatcgt ggcggcccgg
     1981 tgccttcggt gcattccttc gcggaatcta ctatgggatg gatttttctt gccgtaatgg
     2041 cggccacttt gttcatttcc ttatctattt ttgtattccg atatgacact ttgaaaagcc
     2101 gatcaggatt ggattcaata atctctcgag aatcggcgtt tttagcccaa aatgtgcttt
     2161 ttctacttat agcttttgta accctctggg gcaccatatt tcccatattc tcggaagctg
     2221 tggatgacac taccatgact attggccagc cattttttaa caaagttaac gggcccctta
     2281 tgctcttgtt aattcttttg atggggattg ggcccgtact tccttggaga aaagcggggt
     2341 ggacatcaat aaacaagatg ttgcggctac ctttcgtggt cgcttccttg gtattggtat
     2401 caagtctgat aagtggaata acgcaatatt gggttcttat atctttcgtg gcatgtagtg
     2461 tagctattac aacagtgtgc caggaattaa taagaggaac tagatcaaga cattcacatg
     2521 gagaaaattg ggttttatcg ttcttctctt tgatcggggc gaatcgccct agatacgggg
     2581 ggtatatagt tcacttgagc atagttatgc tcgctattgg tataacagct tcctcctttt
     2641 atgatgttca aaaagacgtt gttatgaagc cgggggactc tgagagtttg ggaaaatata
     2701 ctttcaaata tattgatgtg tcagaaaaaa catactcgga taggatggag gaaacggcta
     2761 aattcgaagt gtggtcagcc gataattaca ttggatatat gtacccctac cgcgccgttt
     2821 accctgaatt tcaaatagct gctactaggg gggccataca tagcaccata ttggaagatt
     2881 tttatcttgt tccctcagaa tttagagagg atggacaggc agtgtttagg gtattaataa
     2941 acccattggt tttatggatg tgggcagctg gcccggtgtt gattttagga actttgattt
     3001 ccttatcacc taacaatcgg agtcacagga aaagagtaag tttgccatct atcgatgtgg
     3061 atcaaaaccc ttttgggatg gcaaagtaaa tgctggtagt atctgcgatt atcataagtg
     3121 tgattcctgc tattttgatt gcctatccag tgcttaaaaa gattgcctca agaatgccac
     3181 ttcccgaaga tgaatcctct cttcgtgcag atttagagag aaggtgggaa tcggccctgg
     3241 agggaatccg gcatgctgaa cttgagatgt cgataggcaa cgtaagtgaa tcggactacc
     3301 tatggttgaa acaggtacat atggctgagg cagcgaatgt tttaaaaact ttggaaattt
     3361 ctgcggaaaa agaaaggctg ctaatagagg aaatttcttc agcagccaat acaagatcac
     3421 ttgattcttc ctttgaaaat ggtcatgaaa aggaagattg gaattgatta tacgaacatt
     3481 tgctgtagtt gccttgctgc tattttctct ccttcacact gatacttcat ttgctgagga
     3541 aattgaaata g
//
LOCUS       DASCNS010000058         1571 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000482586, whole genome shotgun sequence.
ACCESSION   DASCNS010000058 DASCNS010000000
VERSION     DASCNS010000058.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000482586"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="QF838_02325"
     CDS             <1..314
                     /locus_tag="QF838_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013253.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HJO59561.1"
                     /translation="VDGKAEIVLDGGVQRGSDVVKAVAMGATAVAIGKMQGWGLAAGG
                     VPGMVRVLEILEEEIRIAMGLMGVTAMDQLNPNYVCPADLVTMPHEMSSWVNMSEDRI
                     P"
     gene            complement(319..681)
                     /locus_tag="QF838_02330"
     CDS             complement(319..681)
                     /locus_tag="QF838_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59562.1"
                     /translation="MTTLLLLSNNLMFLPRIQMAAGSSVSVIRVSDLDRLNKLINESD
                     IDFVVVDLEYDQDFWSNALQVFQENETLNPKIIAYGPHEDTTSMEIARSLGCDPVLAK
                     GAFVNNLKSILAGESKNP"
     gene            772..>1571
                     /locus_tag="QF838_02335"
     CDS             772..>1571
                     /locus_tag="QF838_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59563.1"
                     /translation="MLLKIQASDEPGSILGAIFNHFPAASDGKWNILIGFEAFLAVLI
                     SSIIAMSFAIPQAGLASIALACAAIVPRVNQIQRVNRDRIWDLGMSGWEANRKSIISV
                     TILFIGMVLAYAVVGLVLPQATLQEQYTFIMDRGTAYTSTDLSPERFAHGMTFLRINT
                     YVLIVFFIFAFIYRGLGTSMALGWNAGVWAITLVTAVKVNMAAAASPILLALIATVAL
                     SPHVLLEGLAYLCGSLAAIFFSRGVTLYKPTDSRFFKVLNAVVVLAVVS"
BASE COUNT          403 a          285 c          389 g          494 t
ORIGIN      
        1 cggttgatgg taaggccgag atcgtgttgg atggtggggt gcaaagaggt tctgatgttg
       61 tgaaggcggt ggctatggga gccacagctg tcgcaatcgg taagatgcaa ggatggggac
      121 tagcagctgg aggcgttcca ggtatggtga gggtgctaga aatactcgaa gaagagataa
      181 gaatcgctat gggattgatg ggtgtcacgg cgatggatca actcaatcca aattatgttt
      241 gccctgctga cttagtaaca atgccccatg aaatgagttc ctgggtgaat atgtctgaag
      301 atagaattcc ttaaggattc atggattttt agactcgcct gcgagaattg attttaaatt
      361 gttcacgaat gcacctttgg cgagtacggg atcgcaacct agtgaacgag caatttccat
      421 agaagttgtg tcttcgtgtg gcccgtaggc tattatcttg ggattcaggg tttcattttc
      481 ttgaaaaact tgtagtgcat ttgaccagaa atcttggtca tattccaagt ctacaacaac
      541 gaaatcgatg tcagattcgt tgattaattt gtttaaccgg tcaagatcag aaactcttat
      601 tacagaaaca ctggaaccag cagccatttg aattctggga agaaacatca gattgtttga
      661 taacaagagt aatgttgtca ctatttacct ttcgtattaa ttaatttaag cgtattttaa
      721 tacgagtcat atcattttat gtaaataggc tatcaattgg ggtgattgga tatgttatta
      781 aaaatacaag cttcagacga gccgggatct attttagggg caatttttaa ccattttcct
      841 gcggcttctg acggtaaatg gaatatatta attgggtttg aagccttttt ggctgtcctg
      901 attagttcta tcatagctat gtcctttgct attcctcagg cgggattggc ttcaattgca
      961 ctagcgtgtg cagctatagt cccccgcgtt aaccagatac agagagttaa ccgcgatcgt
     1021 atatgggatt taggaatgag tggatgggaa gctaatcgaa agagcattat ttccgtgacg
     1081 atattattta ttggaatggt gctagcctat gccgtggtcg gacttgtttt acctcaagct
     1141 actctccagg aacagtacac ttttatcatg gacagaggca cagcctatac cagtactgac
     1201 ctgtctccgg aaagatttgc tcacggaatg actttcttgc gaatcaatac ttatgtgttg
     1261 atagtttttt tcattttcgc ttttatatac agaggccttg ggaccagtat ggctctaggc
     1321 tggaatgccg gtgtttgggc tataactcta gtaacggcag ttaaagtgaa tatggcggca
     1381 gcggccagcc ctatattact ggctcttatt gcgacagttg ctttgagccc acatgttctg
     1441 ctggaaggcc ttgcttacct gtgtggttct ctagcagcta ttttcttctc taggggagtc
     1501 actttatata agccgacgga ttcaaggttt ttcaaggttc taaatgctgt agttgtcctg
     1561 gcggttgtat c
//
LOCUS       DASCNS010000059         2456 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000483651, whole genome shotgun sequence.
ACCESSION   DASCNS010000059 DASCNS010000000
VERSION     DASCNS010000059.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000483651"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(97..423)
                     /locus_tag="QF838_02340"
     CDS             complement(97..423)
                     /locus_tag="QF838_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59564.1"
                     /translation="MPSKGKQRASRQAQLRNKRRKSSKSQVVDSRVTQNSSSSAQVDS
                     DIEIELIVSTSETKPIRQNTGRPAQVGTNVLRYEYLGSEIKRISVAAAVIVILLVALS
                     FSSITA"
     gene            complement(470..1372)
                     /gene="xerD"
                     /locus_tag="QF838_02345"
     CDS             complement(470..1372)
                     /gene="xerD"
                     /locus_tag="QF838_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HJO59565.1"
                     /translation="MIAELNTITSLIEDFLEYLVVERACSNNTLVSYRSDLNQFNEHV
                     NLTHVNEIAARHIDNYVKYLSYKGYLSTTRSRKLASVRSFVSYLHSEGIIDHDPTDLI
                     PTPKRSQHLPKVLKLSDVKKLLQLPLDVSSAPDAMRDAAMLQIAYASGLRVSEIVGLD
                     VEDVDFTVGSVRCMGKGSKERIVPVHRIALDSIDIYVRRGRVIFNQQQSKALFLNRRG
                     NRLTRQGFWGIVKKYSKLIGLENSMTPHTLRHSFATHLLEGGASIRHVQELLGHASIS
                     TTQIYTHLALEFVRDQYDNAHPRA"
     gene            1493..2368
                     /gene="lgt"
                     /locus_tag="QF838_02350"
     CDS             1493..2368
                     /gene="lgt"
                     /locus_tag="QF838_02350"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HJO59566.1"
                     /translation="MIEIVTGPFLFESSSFLLSWHGVFSFIAVATAVFLVGRWASLRN
                     INPDDVYSIAVWGIICGIIGARLVHVIDNLQTGPNYLDNPLQIFAIWSGGIGVWGGIL
                     GGAIGGITYGLVKKMPVMQIADLTAPAMLMAQSIGRLGDIVNGEHCAKATDYFFGFIW
                     THAQSAAVYCANTGLNASVQPVVAFEIIWNLTSLMLIWNLKDRIKPAGMLFCLYLALY
                     SVGRFLITFLREDRIWGMGLQEAHFIAIAVLAAMIPLLVFKARFGNADQIRNEMIEAV
                     PQPSRAERRRTQNSD"
BASE COUNT          692 a          515 c          506 g          743 t
ORIGIN      
        1 aataaacccc aggaccggtg ggtacatgag ctagtctttc ggtgaatcta tctaacccca
       61 taaattagcc agcttgaacg tactttgagt gtatctctac gccgttatag atgaaaaaga
      121 taacgcaacc agcaatataa ctataacggc cgcggctaca gatatccgct tgatttcact
      181 ccctaggtat tcatatctca acacatttgt acctacttga gccggtctgc cggtattttg
      241 acgtattggt ttcgtttcgg aggtagaaac tattagttca atttcaatat ctgaatcaac
      301 ttgagcggaa gaggaggaat tttgcgtcac tcttgaatca actacctgac tcttcgaaga
      361 ttttcgcctt ttgtttctta gctgcgcttg tctggatgcc ctttgctttc ctttacttgg
      421 cattttgaaa ctcctccgat tctataaatt taatttgctg acaacctgct tatgctcttg
      481 gatgagcatt atcatactga tctctaacaa actcgagcgc caaatgtgtg taaatctgcg
      541 ttgttgaaat actagcatgc ccgagcaatt cttgtacgtg tcttatagac gctccacctt
      601 ccaggaggtg agtagcgaag ctatgcctga gggtatgagg ggtcatggaa ttttctaacc
      661 ctatcaattt tgaatatttc ttcacaatac cccagaagcc ttgtctagtg agtctgttgc
      721 ctcttctatt caaaaataac gccttagatt gttgttgatt gaaaattacc cgacctcttc
      781 gaacataaat atctatacta tccaaggcaa tcctatgaac aggtactatc ctctctttgg
      841 agcctttacc catacatctc accgacccta cagtgaaatc tacgtcctca acatctaggc
      901 ctactatttc gctaaccctt aacccactgg cataagctat ctgaagcata gcagcatctc
      961 gcattgcatc tggggcggaa gacacatcta agggaagttg taatagcttc ttgacgtctg
     1021 ataatttcaa aactttaggt aggtgctgag atctctttgg agttggaatt aggtcagtag
     1081 ggtcatggtc aataatccct tccgaatgta aataagaaac aaatgatctg acggaagcta
     1141 atttcctact cctagttgta gaaagatatc ctttatatga taagtacttt acgtagttgt
     1201 ctatgtgcct ggcagcaatc tcattaacat gggtcaaatt aacatgctca ttaaactgat
     1261 ttagatcgct tctgtaagac accaaggtgt tgttagaaca agccctctca acgaccagat
     1321 attctagaaa atcctcaatt aatgaggtaa tggtatttaa ttccgctatc attaactaat
     1381 ccaaacttac caaccgaaaa tcatttacgt taaatcttac cttgacatac cttctgggca
     1441 ggataagaat taatcctaat tattgaggag ttctaaattt cattgaagca acatgattga
     1501 aatagtaaca ggaccatttt tatttgagtc ttcttcattc ctgcttagtt ggcatggggt
     1561 gttcagcttc atagctgtgg ctacagctgt gtttctcgta gggagatggg cttcactgag
     1621 aaatataaac ccggatgacg tgtattcaat agccgtgtgg ggaattattt gcggaataat
     1681 tggagcaagg ctagtgcatg tgattgacaa cctccaaact ggtccgaatt atttagataa
     1741 tccccttcaa atctttgcta tatggtcagg gggcataggg gtttggggtg gtattttagg
     1801 gggagctatt ggaggtataa cctacggcct tgtaaaaaag atgcctgtaa tgcagatagc
     1861 cgacttaacc gcaccggcaa tgttaatggc ccagtccatc ggtcgcctag gagacatagt
     1921 caatggggag cattgtgcaa aagctaccga ctatttcttt ggattcatat ggactcacgc
     1981 acaaagtgca gctgtatatt gtgccaacac aggcctgaat gcctcagtac agcccgtggt
     2041 cgcgtttgaa ataatctgga atctcaccag tctaatgttg atttggaatt taaaagatcg
     2101 gatcaaacct gctggtatgt tattttgtct ttacctagca ctttactcgg taggaagatt
     2161 cctaattact tttttgagag aggacagaat atggggaatg gggttacagg aagctcattt
     2221 catagccata gctgtacttg ccgccatgat accccttctg gtgtttaaag ccagattcgg
     2281 aaatgcagat caaattagaa atgaaatgat agaggctgta cctcaaccca gtagggcaga
     2341 aagaaggcga actcaaaata gcgattaagc cggtctgaaa tccgatacta taaattgcag
     2401 attgtacttg ccgttccagt aattcatctc cggtttatat gctacgtcta ccaacc
//
LOCUS       DASCNS010000060         8794 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000489127, whole genome shotgun sequence.
ACCESSION   DASCNS010000060 DASCNS010000000
VERSION     DASCNS010000060.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000489127"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="QF838_02355"
     CDS             complement(<1..190)
                     /locus_tag="QF838_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59567.1"
                     /translation="MKSEVSQPLSSIRALELSEIWAGPFCGCLLGDMGAEVIKIESIQ
                     RIGRGPITPPPNAGGYPDD"
     gene            complement(187..1407)
                     /locus_tag="QF838_02360"
     CDS             complement(187..1407)
                     /locus_tag="QF838_02360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59568.1"
                     /translation="MPYGALEEIIVLDLSDRLAASFCAKTLGLYGCEVIKVEPLSGDP
                     LRRWTNSNPDNQSGDSPLFLHLNAGKKSITLNLDEAKGQNILAALMKVCDVVVETQRP
                     GHLDKIGLAYNQIKKSNPSVIFTSITPYGQTGPYKDFHYDELTLFAMTGAMYREGLPE
                     REPLRYSGEIAQYFAGNAAAAATTAALFKRTLTGQGDWLDISIQECMAGHPHQIGRRA
                     PFIYAGEPDPRSQPRTSASGAREPYAVGTFQCKDGYFSFLPLGPRMWPNIAKMIDRED
                     LLVDPKYDDTVKRSQNREELEAIFQAWVDDKTREEIFQAVQAAGVPGSPILQPEEVHK
                     NEQFQERGFFQNVGHPNGQTLRMTGDPFRFSNAPTSSIKAAPKLGQNTNEVLTKLLEL
                     SDDQIEILAMEKII"
     gene            complement(1519..5928)
                     /locus_tag="QF838_02365"
     CDS             complement(1519..5928)
                     /locus_tag="QF838_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HJO59569.1"
                     /translation="MSRKYLKPLLTKGPEKTYWLALILILSIASLLRFNGINWDQGFG
                     FTPHPDERAILMKVWEIEFPSLSNLSVLFDRSASTWNPHWFPYGSLPIYLLEILQSIW
                     AALTGSEIFDPRSMARSLSTLADLGTIAGTALLAKVCFGNRVSLMASLLVSFSVIHIQ
                     LANFFAFDTFVSLFAVWTLFFLYRVAQKGRLQDSVIAGILIGLGLASKISFFPILGVF
                     LFSHVIGSISYYFEPKKILFPISIAIKNLGLGLLLGLIAFVLAEPYAILDWNQFISDT
                     TEQSEMVRRIRDYPYTRQYIDTTPYLYQITQLGRWGLGWPLTIIGLIGVISALVCKRH
                     WALGTFTVATIFAFGLLLTLSNSILMILVASGLAFFILIINFLLRGSKSLETTLILSW
                     VIPYALIVGSFEVKFTRYLLPIIPLLVILGSAFLVQLTRSPQNYIKKLGYLGSILVIF
                     ATITFGLAFQNIYATPHPGVAASDWINQNVPLNSSLLKEHWEESLPNLEKYKVRELPI
                     YDPDTPPKLNKMSESLSETDYLIIFSNRLYGTVTRIPERYPLMSGYYNALFSGDLGFL
                     PVHLENSYMSFANIEIYEDSFSRPNLPSVDNPFFPDDKISINGGFADESFSVYDHPKV
                     IIFLNAEKLESTRLKTIIEQKSMDFISVNQEKGGPPYEENTARLMMSESTKASQEKGG
                     TWSNIIHTDSTSNRYPIFFWIACLTLISLISFPIGYLMFSTFDDKGFLFSKTLGLLMV
                     CFTSWILSSLHIMKFGQLSLWIGIALVSTISICITIKKYREIFKYISANWSKIISLEI
                     LFLGSFLAFTLIRMMNPDLWHPYRGGEKPMDMAYLNAVVKSTYMPPYDPWFSGGYLNY
                     YYWGQFIVASLVHLTGITTEVAYNLAIATFFALTTCSVYSIGRNILSRKKNPNKINPV
                     IAGLFSILFVCVLGNLDGLYQVWDSIKYGSNIFTDFDYWRSSRMMLPDPPGHEITEFP
                     FFTFLFSDLHAHLISIPFTLLVMGLALQITRNDLGNLWWKSLPTLSVLGLSVGCLAAI
                     NTWDVPIYTVIAMGSLLIAELRQIGGLNSLVFFRVIWKSAYVLTIAYFSFLPYHLNSI
                     TFFNWIEKTTNTTTFSQFISINGLFLVIAFSWCLYSVFPFLPHQNVIRLSAKHLPERI
                     YRSHPKFLSLILITVLLFGYLIVALSSGMLGGAIPLSMLMIFVLVVCCFLEFKNSKSS
                     YSDSFFPCLLAIGAFSVVIGVDIWRIEGDIDRMNTVFKFYLHVWIILGIPAAYFLYQL
                     ITQMSFSLKSTLSYCWVVFIFLLISSSLIYTIFGTIDRLQDRFYKSVTTFTLDGYEFV
                     RDGIYRDENGDVNLSADMAAIRWIRDNIQGSPVILEGVTPTYRWGSRISIHTGLPTVV
                     GWEWHQQQQRWGYRNEINSRMADVNAIYTTPGFELAENLIAKYGVKYIVIGEVEKLYY
                     PNSGLRKIYEGLNGKLEKIYEADGVIIMKVRDL"
     gene            6071..6991
                     /locus_tag="QF838_02370"
     CDS             6071..6991
                     /locus_tag="QF838_02370"
                     /inference="COORDINATES: protein motif:HMM:NF017280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HJO59570.1"
                     /translation="MGVDRDYRCQVIKPEDFNEFWGLVLDELSSIELNVKCEKDELRS
                     DSEITVYQVFYDSLDHVRISGWYSVPNTPVNGLPAILLVPGYQSDPPIPKDWSAKGYA
                     CLSVNPRGKVRSRSQFDPGYPGLLTHRIVDKNTYSYRGFYADAWRGIDFLLSRGEVDS
                     ERIGVAGSSQGGGLTITTSAMRKEIKAASAGAPYLCGFMDAIKLTDTYPYQEINDYLR
                     TYPDRLEEVEGTLAYFDGISFADQIDCPIIMNLGLQDNVCPPETGYALFEIIGSKDKK
                     LFEYEGHGHEAGRYVHSGIMDQFFDKHLKG"
     gene            6999..7916
                     /locus_tag="QF838_02375"
     CDS             6999..7916
                     /locus_tag="QF838_02375"
                     /inference="COORDINATES: protein motif:HMM:NF017280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HJO59571.1"
                     /translation="MSDNRPLDFGQFWYSLGKELSEVPIAAETETLPMRSTDFADLYG
                     IHISSIGPYTIYGYLSIPKGDGPFPVIYYVPKNASVLEIIPQGTSNQIRSRYITFSLA
                     CRGMRTADSPYAAMYPGQLTEGIESLSSYIYRGIVADTLRGLDFLLTCPMIDRSAIVA
                     WGNDNALLAGALHGAVTHIVSTPSFLYDTINQASRTSSYPLEEFNDYLRHNPERKGQI
                     SEILSYYDLRFHSSAVTAESLIMADYDGGLFDKKTLRNLTENMNGAVTVHSSERSSYK
                     DGMFCEKWITDKLFGKDATPIVPNHWQLE"
     gene            7934..>8794
                     /locus_tag="QF838_02380"
     CDS             7934..>8794
                     /locus_tag="QF838_02380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59572.1"
                     /translation="MGLKDQLVPSGISVPQSFIDGPVDMDLIKEFVIAAEDLEYQGLW
                     VQEKIIGGISSLEPINLLSYISGLTSVAALGVAVLIGSTRNPILLAKELATLDQMSNG
                     RLILGYALGGNLKNYRLMDGPDSKRVRHFIESLHVIKALWTEDSPNIQGDFWTFDDLP
                     MYPKPVQKPHPPIWFGGRHPAALKRSARYADGWMGAGSTTSGQFIEHVKIIRTELNRL
                     NRSSDDFKISKRVYVAIDDNKSRAEDRLRTWFKANYGNSDLGSQVSVWGPSDEIVPEL
                     QHLIDSGAGML"
BASE COUNT         2669 a         1696 c         1904 g         2525 t
ORIGIN      
        1 catcatctgg ataccctccc gcgttggggg gaggggttat tgggcctctt ccgattctct
       61 gaatggattc aatcttgatt acctcggccc ccatatcacc aagtaggcag ccacagaacg
      121 gtccagccca tatttcagac agctcgagag ctcttatgga agataatggc tgactcacct
      181 ctgatttcat atgatttttt ccatggccag gatttctatt tgatcatcag ataattccaa
      241 taactttgta agcacttcat ttgtattttg gccgagttta ggggccgctt taatcgatga
      301 agtcggcgca ttcgaaaacc ggaaaggatc acctgtcatg cgtagggttt ggccgtttgg
      361 atgacccaca ttttgaaaga atcctctttc ctggaattgt tcatttttgt gaacttcttc
      421 aggctggagg attggtgatc cagggacacc agcagcttgg acggcttgaa aaatttcttc
      481 cctagtttta tcgtccaccc aagcttgaaa aatagcttct aattcttccc tattttgtga
      541 tcttttgacg gtgtcatcat atttcggatc tactaacaga tcttctctgt caatcatttt
      601 ggctatattt ggccacatcc ttgggcctaa aggaagaaaa ctaaaatacc catctttaca
      661 ttggaaagtt cccactgcat atggctcacg ggcaccagat gctgaggttc tcggttgcga
      721 tctcgggtcc ggttcaccgg catatataaa tggtgctctt cttccaattt ggtgagggtg
      781 acccgccatg cactcttgga tggaaatatc taaccagtct ccctgaccgg taagggttct
      841 cttgaataga gccgctgtag ttgcggcggc agcagcgtta cctgcgaaat attgggctat
      901 ttcccctgaa tatcggagag gttctctctc aggtaaacct tctcggtaca tagctcctgt
      961 catagcaaac aaagttaatt catcatagtg aaaatccttg tacggtccgg tttgaccgta
     1021 aggtgtaatc gaagtgaaaa taaccgaggg gttagatttc ttaatttgat tgtatgccag
     1081 cccaatttta tctagatgcc ccgggcgttg ggtttcgact acgacatcgc aaactttcat
     1141 caaagcggcc aaaatatttt ggccctttgc ctcatctaaa tttagggtga tacttttttt
     1201 ccctgcattc aaatggagaa acaaggggct gtcaccactc tgattatctg gattactgtt
     1261 ggtccacctt cggagagggt ccccactaag tggctccact ttaatcacct cacatccgta
     1321 caatccgagt gtttttgcac agaaagaggc agccaacctg tctgacaggt ccaaaactat
     1381 gatttcttcg agggcaccat aaggcatatt ttagatataa actctagcta aatagtaagt
     1441 gccattgtcc atatgtatgt aaggggtgtc aaacttcagg gtctagttca aaaagtggtg
     1501 gcacgaaacc caaatttgtt ataaatcccg caccttcata ataataaccc catcggcttc
     1561 atatattttc tcgagtttcc catttaagcc ttcatatatt tttcttaatc cactgttagg
     1621 gtaatacagt ttttcgacct ctcctatcac tatatatttg actccatatt tggctatgag
     1681 attttctgct aactcaaagc caggtgtggt gtaaatagca tttacatcgg ccattcggga
     1741 gtttatttca ttgcgatatc cccacctttg ttgttgttga tgccattccc atccaacgac
     1801 tgttggaagg ccagtatgga ttgagattct gcttccccac cgataagtcg gggttacacc
     1861 ttccaaaatt actggggatc cttgaatgtt gtctctaatc caacggattg ctgccatatc
     1921 ggcggataaa tttacgtcac cattttcatc cctataaatg ccatctctga cgaattcata
     1981 cccatcaaga gtaaatgttg ttaccgattt gtagaaacga tcttgtaatc ggtcaatcgt
     2041 tccaaaaatt gtataaatca ggctgcttga tataagtaga aagataaaaa cgacccagca
     2101 gtatgagaga gtcgatttaa gtgaaaaaga catttgtgta attaattggt ataagaagta
     2161 ggctgctggt atacctagaa taatccaaac atgcagataa aacttaaata cggtattcat
     2221 cctgtcgata tcaccttcaa ttctccagat atctacacct atcacgactg agaaagcccc
     2281 gatggccagc aggcaaggaa aaaacgaatc agagtaggaa gatttggagt tcttaaattc
     2341 caagaaacaa caaacaacta atacgaatat catcagcata gatagaggaa tagcgcctcc
     2401 caacattccg gatgacaatg cgactatgag atatccgaaa agcaagacag taatcaagat
     2461 caaagaaaga aacttgggat gggatctgta tatcctttct ggtagatgtt tagccgaaag
     2521 tcgaatcacg ttttggtgag gtaggaatgg aaacacggaa taaaggcacc aagagaaggc
     2581 tatgaccaaa aataaaccat ttattgatat aaattgggaa aatgtcgtgg tatttgttgt
     2641 tttctctatc caattgaaaa aagttatcga attaaggtga taaggaagga aggaaaagta
     2701 agctatggtt aatacatatg ccgatttcca aatcacccta aaaaatacta aagaatttaa
     2761 tcctcctatt tgtctgagtt cagctatcaa taaagacccc atagctataa ctgtataaat
     2821 aggtacgtcc cacgtgttta tggccgccaa gcatcctaca gacaggccta ataccgataa
     2881 ggttggcaag gatttccacc ataaattacc gagatcattg cgcgttattt gtagtgctaa
     2941 tcccattact agaagggtaa atggtatgga aattaagtgg gcatgaaggt ctgaaaaaag
     3001 gaatgtaaag aaagggaact ctgtaatttc atggccaggt gggtcgggca gcatcatgcg
     3061 actgcttctc cagtagtcaa agtctgtaaa aatattagat ccatatttta tagaatccca
     3121 aacttggtaa agtccatcaa gattacccaa aacacatacg aaaagaatag aaaataatcc
     3181 ggctatcaca gggttaattt tgttcggatt tttctttctg gaaagaatgt tccttcctat
     3241 tgaatacaca gaacaggtgg ttaaagcgaa gaaagtcgcg atcgctaggt tataagctac
     3301 ttctgtcgta attccggtca gatggaccaa agaggctacg ataaactgac cccagtagta
     3361 gtaattcaag tatcctcctg aaaaccacgg gtcatatggg ggcatatagg tagatttaac
     3421 caccgcattg aggtaggcca tatccattgg tttttccccg cccctgtaag ggtgccagag
     3481 gtcagggttc atcattcgga taagagtaaa tgctaaaaaa gagccgagga ataggatttc
     3541 cagacttatt atttttgacc aattcgctga gatatatttg aaaatttccc gatatttctt
     3601 aattgttata catatcgaaa tcgtagatac caatgctatg ccgatccaga gagacaattg
     3661 accaaatttc attatgtgca gactagataa aatccaggat gtaaagcaca ccattagtaa
     3721 gcccaatgtt ttcgaaaata agaatccttt gtcgtcaaat gtggaaaaca taagataacc
     3781 aataggaaat gaaattaatg aaataagtgt caaacaggct atccaaaaaa agatggggta
     3841 ccggtttgat gtagaatcag tgtgaatgat attagaccag gtgccacctt tctcttgact
     3901 tgctttagtc gattccgaca tcatcaaccg ggctgtattt tcttcgtagg ggggaccgcc
     3961 tttctcttga ttaacagata taaaatccat cgatttttgt tctatgatgg tttttaatct
     4021 agtagattct agtttttcag catttaaaaa aatgattact ttagggtggt catatactga
     4081 aaaactttcg tctgcaaaac caccattgat acttatcttg tcgtcgggaa aaaatgggtt
     4141 atccacagat ggtaaattag gcctggaaaa agaatcttca taaatttcga tgttcgcaaa
     4201 agacatataa gaattttcta aatgaacagg tagaaatcct aaatctcccg agaataaagc
     4261 gttgtagtat ccgctcataa gagggtaacg ttctggaatt cgggtgactg ttccgtagag
     4321 gcgattactg aaaattatca aataatccgt ttctgatagg gattccgaca ttttatttaa
     4381 tttagggggg gtatctgggt cgtatatcgg aagctcacgc accttgtatt tttccaaatt
     4441 gggaaggctt tcttcccaat gttcttttag taaggaagag ttgagtggta cattttggtt
     4501 tatccaatct gaggctgcga ccccaggatg tggtgtggca tagatatttt ggaaggctaa
     4561 cccaaatgta attgtggcaa atatgactaa aatagacccg aggtagccta actttttgat
     4621 gtaattttgg gggctacgtg ttagctgtac taagaaagcg ctcccaagta ttaccaacaa
     4681 tgggattatt gggagtaaat atcttgtgaa tttaacttcg aagctgccaa cgatgagagc
     4741 ataaggaatt acccaagaaa gaattagcgt ggtctctaac gacttggatc cccttaataa
     4801 aaagttgatt attaggatga aaaatgcaag gccagaggca accaaaatca tcaggattga
     4861 attcgataag gttagtaaaa ggccgaaggc gaagattgtt gccactgtga acgttcctaa
     4921 ggcccagtgt cttttacata ccagggccga aatcactcct atcagaccta ttatcgtaag
     4981 cggccatcct agcccccatc gacctaattg agtgatttgg tacaagtaag gagtggtgtc
     5041 tatgtattgc cttgtatatg gataatccct gattctccgt accatttctg attgttctgt
     5101 agtgtcagaa ataaattggt tccaatcaag aattgcatag ggctcggcca atacgaaagc
     5161 aattaagcct agtagcaggc ctaaacccag gtttttaatt gcaatggata tcgggaatag
     5221 aatctttttc ggttcgaaat aataggaaat ggaacctatc acgtgggaga acaagaacac
     5281 acctagaatt ggaaaaaaac ttatctttga tgctaaacct aacccaatca atatcccggc
     5341 tatcacagag tcttgaagcc tacctttttg ggcaacccga taaaggaaaa atagagtcca
     5401 tacagcaaag agagatacaa aagtgtcaaa agcaaaaaaa ttcgctaact gaatgtgtat
     5461 aacagaaaat gacaccaata aagatgccat aaggctaact cgattaccga aacatacctt
     5521 tgctaaaagt gcggttccag ctatggtccc aaggtcagcc aaggtagaaa gagacctggc
     5581 catacttcta ggatcaaata tctcagatcc ggtaagtgct gcccaaattg attgaaggat
     5641 ctctaacaaa taaataggta agcttccgta agggaaccaa tgggggttcc aggtgcttgc
     5701 tgagcggtca aataatactg aaaggttcga aagtgaaggg aactcaattt cccaaacttt
     5761 cattaaaatg gcccgttcat cagggtgggg agtaaaacca aagccttggt cccaattgat
     5821 gccattaaag cgtaaaaggg aggcgatgga tagtattagt atcaaagcca accaatatgt
     5881 tttttcggga cccttagtca ataaaggttt taggtatttt ctactcatta tttcattggt
     5941 tcattctata attctttacc aactcgccac cttatatcag atgattgact gagttgacca
     6001 ttgaggtaaa atcctttgac ttgcgatgtg atggattgcg tccttttctg aatttgggag
     6061 gagtaaaaat atgggagtgg acagagatta tagatgtcag gttatcaaac cagaagattt
     6121 taacgaattt tggggattgg tcctagatga actttcatca atagaactta atgttaagtg
     6181 tgaaaaagat gaactcaggt ccgatagtga aattactgta tatcaagtct tttatgatag
     6241 tttagatcat gtgcgaatat ccggttggta ctctgtccct aatactccag ttaatggatt
     6301 accggccatt ttgctggttc caggttacca aagcgaccca ccaattccta aggattggtc
     6361 tgccaaagga tatgcttgtt tatcagttaa tcccagaggg aaagtcagga gtagaagtca
     6421 atttgatcct gggtatcccg ggttgctaac tcatcggata gtagataaga atacatattc
     6481 ttatcgaggt ttttatgccg atgcttggcg aggaattgat tttttgttga gtagaggaga
     6541 agtggacagt gagaggattg gtgtggctgg tagtagccaa ggcggtggcc taaccattac
     6601 cacatcagct atgagaaaag aaatcaaagc tgcttccgca ggtgctccat atttgtgtgg
     6661 atttatggac gcaatcaagt taacagacac ttatccctac caagagatta atgactatct
     6721 tcgtacttac cctgacagat tagaagaagt tgagggcacc ctggcttatt ttgatggtat
     6781 atcgtttgcc gatcaaattg actgtccaat tatcatgaac ttaggattgc aagataacgt
     6841 gtgccctcct gagacagggt atgcactttt tgagattata ggcagtaaag ataaaaaact
     6901 ttttgaatat gaaggccatg ggcacgaggc aggaagatac gtacattcag gcatcatgga
     6961 tcagtttttt gataagcatt tgaaagggta gtgaagaaat gagtgacaac agaccgttag
     7021 attttggcca attttggtac tccttaggaa aagaattgag tgaggtgccg atagctgcag
     7081 agacggaaac ccttcccatg cgttcaacag attttgcaga tctatacggg attcatattt
     7141 caagcatagg gccatacacg atatatggct acctgagtat tccgaaaggg gacggccctt
     7201 ttccggttat ttattatgtg cctaaaaatg ccagtgtttt ggaaataatc ccacagggaa
     7261 cttccaatca aataagaagt agatatatta ctttttcact agcctgccgt ggaatgagaa
     7321 ctgcagatag cccatatgcg gctatgtatc caggacagct tactgaagga atagaatctc
     7381 tctcttcgta catttacagg ggaatagtcg cggatactct tagaggacta gattttttgt
     7441 tgacatgccc aatgattgat aggtcggcaa ttgtcgcctg gggaaatgac aacgctttgc
     7501 tggcaggagc actacatggc gcagtaacac acatagtttc aactccctca tttctatatg
     7561 acacaattaa ccaggcctcc agaacttcct cataccctct tgaggagttt aatgattatc
     7621 tccggcacaa cccagaaaga aaaggccaaa tatcggaaat tcttagttat tatgatttga
     7681 gatttcatag ttcggctgta accgctgaat ccctcattat ggccgattat gacggaggtc
     7741 tttttgacaa aaaaacttta cggaacctca cagaaaatat gaatggtgcg gttacagttc
     7801 actcctctga acgatccagt tataaagatg gtatgttctg tgagaaatgg atcacagata
     7861 aattatttgg taaagacgca acacccatag ttcctaacca ttggcagcta gaataaattt
     7921 cttggagttt gaaatggggc ttaaagatca attggtacca agtggtattt ccgttcctca
     7981 gtcctttatt gatggtcctg tggatatgga tctcataaag gaatttgtga tcgcagcgga
     8041 agacctggaa tatcaagggc tatgggtaca ggaaaaaatt ataggaggga tatcttccct
     8101 agagcctatt aacctactta gctatatatc tgggttgaca tcagtagcag cactaggagt
     8161 tgctgtactt atagggtcca ctagaaatcc gatcttattg gccaaagaat tggctacatt
     8221 agaccagatg tcgaatggga ggttgatctt aggatatgcc cttggaggta acctgaaaaa
     8281 ttaccgattg atggatggtc cggattccaa gagggtgcgc cattttatag aatcattaca
     8341 tgtaataaag gctttatgga cggaagattc tccgaatatt caaggtgact tttggacttt
     8401 tgatgatctc ccaatgtatc ccaaaccagt acaaaaacct catcccccga tatggtttgg
     8461 tggtcgtcat cctgctgcct tgaaaagatc tgctaggtat gcagatggtt ggatgggggc
     8521 gggttcaacg acatctggcc aatttataga acatgtaaaa attattagga ctgagttgaa
     8581 tcgtcttaat agatcaagtg acgatttcaa aatctctaaa agagtttatg tcgccataga
     8641 tgataataaa tccagagctg aagataggct gaggacctgg tttaaagcaa actatgggaa
     8701 ctcagactta gggtcacaag tatctgtctg gggacctagt gatgaaattg tccctgaact
     8761 acagcaccta attgattcag gtgcaggtat gttg
//
LOCUS       DASCNS010000061        14992 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000499037, whole genome shotgun sequence.
ACCESSION   DASCNS010000061 DASCNS010000000
VERSION     DASCNS010000061.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14992)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000499037"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="QF838_02385"
     CDS             complement(<1..771)
                     /locus_tag="QF838_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59573.1"
                     /translation="MNFSSQLYLHLVFLGLAIFCFIFLSTVTLASDSDIKIKSASAES
                     EFPEGIRFSVQAISNSRISEIAVNFRIGQQVSGVYEYLDFEDALEVEGSLFWKTNTSS
                     KYVPPGTIIEYSFSISNESGNTFDSEKKYLVYSDTRFEWQEVELGTISVAYHGPVKSR
                     AEDLLDAMNQTLYSMEPVFGDVQGDPIRVTMYNNVAEMMGALPPSSSTIRSELITEGQ
                     AYPDIGTILVLGGGRLSRGTASHEVTHILVHRAGDSVFS"
     gene            complement(774..998)
                     /locus_tag="QF838_02390"
                     /pseudo
     CDS             complement(774..998)
                     /locus_tag="QF838_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024590.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(1011..1466)
                     /locus_tag="QF838_02395"
     CDS             complement(1011..1466)
                     /locus_tag="QF838_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59574.1"
                     /translation="MQILNYSDREIVKNRFENELVDEVSITLFTQTDLGGLIVPGREC
                     ETCAPTEQLLREVSETSELINFRKLDIRSDSEEAERCNVSRIPSFLVSKRDETNVRYI
                     GMPAGTEFPVLMEALVNVSSGEPKISEETKGFLEDLEENVSIKTFVTPN"
     gene            complement(1485..2129)
                     /locus_tag="QF838_02400"
     CDS             complement(1485..2129)
                     /locus_tag="QF838_02400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HJO59575.1"
                     /translation="MTARENLRELLYKYYEKPFAAFFDRIGFTPNMVTLLGLLIILIS
                     AIFISVGYFWLGGIIVAIGGALDLIDGALARKKGLVSKFGAMFDSVADRVQEALVLLG
                     LLIYYSLNDCVSTDLVPFNLLEGFNSCAFAQIMTYTAFVGSVMVSYLRSRAEGLGIEC
                     KVGIMTRPERVAIISVGLVIAHWVPIVMIIVLMILTGLGFFTTIQRLMHSSKEL"
     gene            complement(2157..3311)
                     /locus_tag="QF838_02405"
     CDS             complement(2157..3311)
                     /locus_tag="QF838_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HJO59576.1"
                     /translation="MNQPLKVALVSPYDFASPGGVTTHIQNLSVNLEQRGVFTRVFAP
                     VSAMSHNSISNLIPMGVPVSIPIGGSIARISLSVWLAGRLRKSIETENFDIVHVHEPF
                     AGALTLAVLAKQLQNNPLMVATFHSFEGSGLYQIVPNKLLNKYFGKIDGKIAVSSAAR
                     SYVSKYLPADYDIIPNGINTDEILMATPFQNLLDDKVNILFVGRLEKRKGLKYLLASY
                     CDLKWKFPNIRLIIVGAGKMDVESQRILGERNPEDVFIAGSVTHEEKLKYFKTADIFC
                     TPATGQESFGIVLLEAMSAELPIVASGIDGYRSVLDEKEGLFTNIKDSHDLSEKLSRL
                     IANKKMRIQMGKNGREKSKLFSWSIVTDKIMDHYLSLLSLRKTDHIRCID"
     gene            complement(3349..4440)
                     /locus_tag="QF838_02410"
     CDS             complement(3349..4440)
                     /locus_tag="QF838_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HJO59577.1"
                     /translation="MPEIKVAIIGVGNCASSLVQGVAKYADAGPNDTVPGLMHPVLGE
                     YGIGDIKVVAAFDVDANKVGKDLSEAIFADPNNTVKFQDVEETGVVVQRGMTHDGIGR
                     YTSEIITKAEGGTDDVAAILKEREVDVVINYLPVGSEEATKWYVEQVLQAGCGFINCI
                     PVFIASGENDYWQNRFREAGAPIIGDDIKSQVGATITHRVLTRLFMDRGVELQRTYQL
                     NFGGNTDFMNMLDRDRLVSKKISKTQSVTSQVDEFIPDRDVHVGPSDYVPWLTDRKWC
                     YIRMEGKTFGDVPLNMELKLEVWDSPNSAGVVIDAVRCAKIAKDRGISGPVTAPSSYF
                     MKSPLIQYTDDEARQLVEDFIAGEESAQG"
     gene            4651..5928
                     /locus_tag="QF838_02415"
     CDS             4651..5928
                     /locus_tag="QF838_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59578.1"
                     /translation="MNLAKFLSKRTPFYYGWLILGTSGSTQIVRNAAASLTLAVFMYP
                     MAEDLGWSRTVLAGAVSVGGLASTFISPAIGWLIDKYGARTVLTSSIMILGVTTFLTG
                     WATIPITFYVTYGIGRIIFSSPVQIGSSVVVSRWFIQKRGRANGILTFCHSIGMTGFP
                     LMASILIGIYGWQVAWHLLGVGVWVIALLPVFLLLAETPESVGLLPDGDKETSIASTE
                     TNTTAIIEEISWTLKEALKTPALWQLALAGGLLFVIHSGTNTHMAAFFQDAGLTSNQA
                     AAAVSLNAIFTGLGGLGWGWTIEKIKARYCYAMVAALMSVCSILFSTADTVTEAWIYA
                     SLFGTALGGMLVVPSVAMADYFGRSSLGTIRGFTEPFVSFSQAVGALLSGLVFDLTGN
                     YNYAFYTLSIVALMAILLTITATVPIHQDNKKG"
     gene            complement(5970..7028)
                     /locus_tag="QF838_02420"
     CDS             complement(5970..7028)
                     /locus_tag="QF838_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HJO59579.1"
                     /translation="MAKYKVVTQKPAGVTFDLADGQYLLEKESLDSIDAEIIEIDAAT
                     EDEFIASAKDADAVIARGRPITKKIIDALENCKVISLGSVGADTVDVNAATAKGIPVT
                     NVPDTFIEEVADHTMMLLLASYRRLNIMDQMVRENRWSEGRPLLSEFPRLWGQTLGFI
                     AFGHVAKAVSIRAKAFGLRLIAFDPYIEELSMTEYGVQPISTLEEMLSMSDIVSMHAP
                     STEDAFHLMSESQFKSMKSTALFINNGRGPTVDEEALIKALENKWIAGAGLDVFEQEP
                     PDPENPLLHMENVISTPHVASASQRMAPETRRRVGKELSLVLTGRWPRSCVNPAVLAD
                     SGLKRWQPYSMGRGPGSG"
     gene            7630..8934
                     /locus_tag="QF838_02425"
     CDS             7630..8934
                     /locus_tag="QF838_02425"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59580.1"
                     /translation="MITKILESRFLAALQYRDYRVLWTGNMSASAAAWALIVARGWIV
                     WEMSESSLYVGLVTFLAMIPRVIIPPFTGYLADRYERRKVMATIYGLNLTHTLFLAGL
                     LAFGQIEMWHLMILAFVNGAARSAQMPVGQALVPNLVPKDKLLNAIALNQATMHGSRL
                     LGPLTILPLLESTRIEWAFLACSMFYVVSLVQSLRIKTPSTGSMDNKKGFFSNFTQGL
                     PYVYRHPLLKLIVMMAFFHCGFTMSFEAVLPVLSVERFNATDGADFSLMMMSIGGGAL
                     ISLIWLSGVTSETTKGKLFLNLGVISGLAPILLAFSPNIYFAIGSAALMGASQAGYMT
                     LTHTMIQLVTDDSVRGRVGAVYSVHIGGIMASMNLANGAISDFAFLKVDLFNGLRVLL
                     PADTMLTIGGILFIATVFISWFIISLRKIYQTGIPQSSLIQA"
     gene            complement(8955..9899)
                     /locus_tag="QF838_02430"
     CDS             complement(8955..9899)
                     /locus_tag="QF838_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012515.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HJO59581.1"
                     /translation="MKKNIININRHRKEPIDVVCIGMVTPAEVYVVEKLPDWNTGTKW
                     ISRADFISDDAAIVACCIAKWGLSSGLVCNKLGDDVSGNNVFDELEALGVVGGFVKDK
                     NLETPYEIVISDSTGGRTYIWDRREDLLTTLEFADTAVISGAKCAYADWYDYPYNLPA
                     IKKAVEKNVPVLINIEDRPQSTESVKELVELATWVQCTPDQTNNTHNKYETCNELLRL
                     GVKGVLFTGSEEGCFWKSKSESFSVRPPSIDLLDANGAGAVLSSGFLYGISKGWDANV
                     SCRFAVSAASLQCQIVSPSAIPKEEVMKVSQGLEVIIS"
     gene            10029..11162
                     /locus_tag="QF838_02435"
     CDS             10029..11162
                     /locus_tag="QF838_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012546.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59582.1"
                     /translation="MIAIVFLSFLAGIIGITAFVTIYYAIETRRQGFKIFWEKLEYFS
                     SIVSIEKDKITFHPRGRDATDGLLPCVGLIGRDGSILIGGPPYQENGIITREIVSKNG
                     SLGQGARIRTSLFIYETNPKIDLGLDYTDVSFDSELGTLTGWHIPNNSNNWVIGVHGH
                     RSYRTESMRFVSTFRKSGLNVLLSDYRNDQNAPLDKGGYHMFGLTEWRDLESAVKFCQ
                     EKGAEKIFLMGHSMGGAIVVKYLLESSLKSLINGAILESPALNLNKIISMKAAEIPLL
                     PRGAEYPVKKLVSWMVKLNWENLNYLSRSDELTTPILLIHSESDVTVPVSLSDQLAEN
                     RSDIVTYLRLKKAHHAAAWNTNKEIVESSMEAFLNKNISSTDD"
     gene            11325..11990
                     /locus_tag="QF838_02440"
     CDS             11325..11990
                     /locus_tag="QF838_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HJO59583.1"
                     /translation="MAKLKGTGMLAVWSEVASEHEEEFNEWYNQEHISERLSVPGVLS
                     AARYEAVKSGPKHLAVYELEHSSVMKSPEYVELKNNPTDWSKRMSPDVIGTKYIRNVY
                     QMIFPTELTEEVVSSPMAPALQIGRMNIPSNTEDEWNNWYNKIYVPNYETVDGVIRGR
                     RYQTVEGSPKYMTFYELDSANASQTEEWQKQQTAHPDNERMRQEMQHESDSPGIWAKT
                     FEL"
     gene            12041..12946
                     /locus_tag="QF838_02445"
     CDS             12041..12946
                     /locus_tag="QF838_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HJO59584.1"
                     /translation="MEWMWFSLASAFTFALVSVLDKLLISKHVDNAKVFIVTVGVAQI
                     CLGLIVIPISAFSGLTLSTLTVVIFSGISSGMYLVIMFQIMESQDVSRVVPVVSTYPV
                     FVAALAFFILGEQVTIYSLACILITVFGAALVSLSPSGKKALAKSDVTAMLLLFLASI
                     LFGLSQFLAKTIADDVDMWTQYMVRGVSGGLTCFSLILLPGVVKGVTSIFQKPKSLFL
                     IGFTEGFLVFFAILFFFLAIYAGEIYMVSTVMASRPIFVFGIGLILSLPMIKLLNEPL
                     NGRVLAVRATGTFLTVLGVAGVSFL"
     gene            complement(13105..14262)
                     /locus_tag="QF838_02450"
     CDS             complement(13105..14262)
                     /locus_tag="QF838_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO59585.1"
                     /translation="MLDYYNVTELLSTEDRQIQNSARDYLDSEVMPGVKDWWEFGEFP
                     KHLVPEFGEMGFLGSNLPAEYGCPGVNNKSYGLIMYELERIDSGFRSFASVQSALVMY
                     PIYRYGSEDQKKKYLPEMAKGNTIGCFGLTEHDGGSDPGAMKTNAKKDGESYILNGTK
                     MWITNGNIADVALVWAKDETDTVRGFLIPTDTPGFTANKIEHKMSMRASVTSELVLED
                     VRVPATSILPESNGLSAPLSCLTQARYGIAWGALGALESVYEDALRFSKARSTFGKPI
                     ASRQLVQDKLVDMVTDHTQGLLLAWRLGEMKDAGTMDYNHVSVAKRENVRSALKAARS
                     AREVLGGSGITLDYGVIRHMLNLETVDTYEGTRDVHTLIIGRHITGENALV"
     gene            14387..>14992
                     /locus_tag="QF838_02455"
     CDS             14387..>14992
                     /locus_tag="QF838_02455"
                     /inference="COORDINATES: protein motif:HMM:NF023128.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59586.1"
                     /translation="MNKLPKSDRKIIRAWCMYDWANSGYATSGLAAFFPVYYVLLFKN
                     ALGEEAVLWGINFTGSSSWSVGLAISTLIVAITSPILGVIADRIPIKKTLLWIYTVAG
                     SLFAVLGFFSAYTSHPWAWLLGTFILGNIGFAGGLVIYNSFLPHIAPRNLLDEISSRG
                     YAFGYIGGGLLLLFHVIVLVLTSQSDHTDLILRLCIASVGLW"
BASE COUNT         4216 a         3343 c         2939 g         4494 t
ORIGIN      
        1 gctaaacaca ctatccccgg ctcgatggac caatatgtga gtcacttcat gagaggcagt
       61 gcctcttgaa agtctgccac caccgaggac taatatagtg ccaatgtcgg gataggcctg
      121 tccttctgtt atcaattcac ttcttatcgt ggagctgcta ggaggtaacg ctcccatcat
      181 ttctgcaaca ttgttgtaca ttgtcactct gattgggtcc ccttgaacat ccccaaatac
      241 aggctccatt gaatataagg tctggttcat ggcatccaac aaatcttctg ctctactttt
      301 aacaggtcca tggtaagcaa ccgatatcgt acctaattct acttcttgcc attcaaatcg
      361 agtatcggaa tatacgagat actttttctc ggaatcaaag gtgttgcctg attcatttga
      421 aatgctaaaa gaatattcaa taatagttcc tggaggcaca tatttggaac tggtgtttgt
      481 cttccagaaa agactacctt cgacctccaa agcatcctca aaatccaaat attcgtacac
      541 tccagatacc tgttggccaa ttcggaagtt aacggctatt tctgatatac ggctattaga
      601 tatagcctgt acagaaaagc gaatcccctc gggaaattcg ctttccgccg aagctgactt
      661 aattttgatg tcagaatcag atgctagggt gacagtgctc aaaaaaataa aacaaaatat
      721 cgctaatccc agaaatacca aatgcagata taattgggaa ctgaaattca aaattagcta
      781 tctccttcca gatctttcag caacgctatt tttacgtgct ccactagcat ttgttcgttt
      841 gccgcaccag taaagctata tttttcatta attacagtta aagggactcc tctaacttga
      901 tatgccttcc ctaaatccgg aaactcttga acctcaataa cttcagccac tactttctgt
      961 gtgaatgctg ccattgcgtg ggtcaaactg gccacacctg ggcaatacgg tcaatttggt
     1021 gtgacaaatg ttttgattga gacattttct tcaaggtctt ctaaaaaacc tttcgtctct
     1081 tccgatattt tcggttcacc agatgaaaca ttaaccaggg cttccatcaa aaccggaaac
     1141 tcagttccag caggcatgcc aatatatctc acgtttgtct catctctctt cgaaactaaa
     1201 aaggaaggta ttctggaaac attacatcgt tcagcttctt ctgaatcact cctaatatct
     1261 agtttcctaa agtttattag ttcagatgtt tcactaactt ctctcaatag ttgttcagtt
     1321 ggcgcgcaag tctcgcattc acgaccagga actatcagac ctcctaggtc cgtttgagta
     1381 aacaaagtta tacttacctc atctactaat tcgttttcaa atcggttttt gacgatctct
     1441 ctatccgaat agtttagtat ttgcatatcc tataatctcc tgggttatag ctctttagaa
     1501 gaatgcatta atctttgaat cgttgtgaaa aaacccagtc ctgtaagaat cattaagact
     1561 ataatcataa caattggcac ccaatgggct ataacaagcc ctacagatat aatagctact
     1621 cgttcaggcc gagtcataat cccaacttta cactctattc ccaacccttc tgctcgcgac
     1681 ctaagatagc taaccataac tgaccctaca aatgccgtgt aggtcattat ttgagcgaaa
     1741 gcacaagaat taaaaccttc cagtaaatta aaggggacaa gatcggtcga cacgcaatcg
     1801 tttagggaat agtaaattaa taggccgagc agtactaatg cctcttgtac tctatctgca
     1861 actgaatcaa acatagcgcc aaatttcgaa accaaccctt tcttcctagc caaggcacca
     1921 tcaatcagat ctagagcccc tccaatggcc acaataattc cgcctaacca aaaatagcct
     1981 acagaaataa atatcgctga tatcaaaatt atcaggagac cgagcaaggt aaccatgttt
     2041 ggggtaaagc caatccggtc aaaaaaagca gcgaaaggct tttcataata tttataaagc
     2101 agttcccgta gattttctct ggcagtcata ttttctctct gcgcataaaa ataatatcaa
     2161 tcaatgcatc tgatgtgatc tgtctttcga aggctaagta atgataaata atggtccata
     2221 attttatcag tcactattga ccaagaaaat aattttgatt tttctcttcc atttttcccc
     2281 atttgtattc tcattttttt attagcaatc agccgtgaca acttttccga caagtcatga
     2341 gaatctttta tgtttgtaaa caaaccttcc ttttcgtcta gcaccgaccg ataaccatct
     2401 attccggagg ccacaattgg taattcagcc gacatagctt ctaatagcac tattccaaag
     2461 ctttcttgac cagtcgctgg agtacaaaaa atatctgcag ttttaaaata tttcaatttt
     2521 tcttcgtgtg taacagatcc cgcgatgaag acgtcctcag ggtttctttc tcccagaatc
     2581 cgttgtgatt ctacatccat ttttccagcg cccacaatga tgagtcttat attaggaaat
     2641 ttccatttta ggtcacaata cgatgccaga aggtatttta aacccttcct tttctcaagc
     2701 cgccccacaa acaaaatgtt taccttgtca tcgagaaggt tttgaaatgg ggtggccatt
     2761 agaatttcat cagtatttat cccgtttggg ataatgtcgt agtctgctgg taaatattta
     2821 ctaacataag atctagctgc tgatgaaacg gcaattttcc catctatttt tccgaaatat
     2881 ttgtttaata acttattagg aactatctga tataggcctg aaccttcgaa cgaatgaaat
     2941 gtagccacca taagtggatt gttttgtaac tgcttcgcca acactgcgag agtcaaggct
     3001 cctgcaaatg gctcatgaac atgaacaata tcgaaatttt cagtctcaat cgatttcctg
     3061 agcctgcctg ccaaccaaac ggatagggat attctcgcta tcgatccacc tattggtatc
     3121 gaaactggga cccccatagg gattaagttt gaaattgagt tgtgagacat tgccgaaaca
     3181 ggcgcaaaaa cccgggtgaa tacacccctt tgttctaaat taacactcaa attttgtata
     3241 tgcgttgtga caccaccagg tgaagcaaaa tcatacgggg aaactagtgc aaccttaaga
     3301 ggttggttca tataacctcg tctatagaca aaccacggat gggtgaaact aaccttgggc
     3361 ggattcttct ccagcgatga aatcctcaac aagttgccta gcttcatcgt ccgtgtactg
     3421 aatgagcgga gacttcataa aatatgacga aggtgcagtt acagggccgc ttatgcctct
     3481 atcctttgcg attttggcac aacggaccgc atctattacg acccctgctg agttagggct
     3541 atcccaaact tcaagcttta actccatatt taaaggcaca tctccaaaag ttttaccttc
     3601 catcctgata taacaccact tccggtctgt gagccacggc acatagtcac tagggcccac
     3661 atgaacatca cggtcaggta tgaattcgtc aacttgcgat gtaacagatt gcgtttttga
     3721 tattttttta gaaaccagcc tgtctctatc aagcatattc atgaagtctg tgttgccacc
     3781 aaaatttagt tggtatgtcc tttggagttc gacgccacga tccataaata atctagttaa
     3841 cactctgtga gtgatagtgg cacctacctg agatttgata tcatccccaa ttattggtgc
     3901 tccagcctct ctgaacctat tttgccaata atcattttct ccactagcga tgaaaacagg
     3961 tatacagttt ataaacccgc agcctgcctg caacacttgt tcgacatacc attttgtagc
     4021 ctcttcagaa cctaccggca ggtaattaat taccacatct acttcgcgtt ctttcaaaat
     4081 cgcggccacg tcgtcggtac caccctcggc tttagttatg atttcagagg tataacggcc
     4141 tatcccgtca tgggtcattc ctctttggac caccactcca gtttcctcaa catcttgaaa
     4201 ctttacggta ttatttgggt ctgcaaagat agcttctgag agatctttac cgactttatt
     4261 tgcatcaaca tcaaacgcag caacaacttt tatatctccg ataccgtact ctcccaaaac
     4321 aggatgcatc aggccgggaa cagtgtcgtt agggcctgcg tcagcgtact tggcaacccc
     4381 ttgaaccaag gaggacgcac agtttccaac tccgattatc gcaactttta tctcaggcaa
     4441 actaataaaa ctcctcatac tgctttttta gagtgaaaat aaatttctca cccaaaaact
     4501 acggcttcat tcataacaat cgaattaaag aaaaatttta gcattgtggg tgcactatta
     4561 acaatacaac attttgaatg tttgaagcag tatcccctac atggtagcat ccctgcggaa
     4621 tatcacattt tgaatccatg aggatcttca ttgaacctag caaaatttct ttcaaaacgc
     4681 acgccgttct actatggctg gcttatttta ggtacatctg ggtccactca aattgtcagg
     4741 aatgccgcag caagcctaac cctggctgtt tttatgtatc caatggcgga agatttaggg
     4801 tggagtagaa ccgtgttggc tggagcggtc agtgtaggtg gattggcatc taccttcata
     4861 tcacctgcta taggctggtt gattgataaa tatggtgcca gaactgtgct tacctcaagc
     4921 ataatgatat tgggtgtaac taccttcctg acaggatggg ctactatccc gatcaccttc
     4981 tatgttacct acggcattgg aagaattata tttagtagtc ctgttcagat tgggtcttca
     5041 gtagtagttt ctagatggtt tattcagaag cgcggaaggg caaacgggat actcactttc
     5101 tgtcattcca ttgggatgac agggtttcca ctaatggcat ccatactgat aggcatatat
     5161 ggatggcaag ttgcttggca tctactgggt gtgggagttt gggttatagc gttacttcct
     5221 gtttttcttt tacttgcgga aactcctgaa tcagtcggtt tactaccgga tggagataag
     5281 gaaacctcga ttgccagtac tgaaacaaac acaacggcca taattgagga aataagctgg
     5341 actttgaagg aagcgttgaa aactccggct ttatggcagc tggcattggc tggaggtctt
     5401 cttttcgtta ttcattcagg aacaaacact cacatggcag cgtttttcca ggatgccggt
     5461 ctaacttcta accaagcagc agcagctgtg agcttaaacg cgatattcac tggtcttggt
     5521 ggtttaggtt ggggttggac tatcgaaaaa ataaaggcac gctactgtta cgcgatggta
     5581 gctgcattga tgagtgtatg ctctatatta tttagcacag ctgacactgt aacggaagca
     5641 tggatatatg cctcactgtt tggcactgcc ctagggggga tgttagttgt gccatccgtc
     5701 gccatggcag attatttcgg tcgcagttct ttagggacaa tcagaggttt cacagaacca
     5761 tttgtatcct tcagccaagc cgtgggggcc cttctctcgg gtttggtttt tgatctcaca
     5821 ggaaattaca actacgcttt ctataccctt tcgatagtgg ccttgatggc aatactcctc
     5881 acaatcacag ctactgtacc cattcaccaa gacaataaaa aagggtgaca aaatcaccct
     5941 tttctagatc tttatttcag caatgtgtat tagccggaac cggggcctct acccatagaa
     6001 taaggttgcc accgcttaag ccccgaatcc gctaatacag ctggattcac acaacttctt
     6061 ggccatcgtc ctgtaaggac taatgaaagt tccttgccta ctcgtctcct agtctcagga
     6121 gccatccttt gcgatgcaga ggcaacgtga ggagtggata tcacgttttc catatgcagg
     6181 agagggtttt cgggatctgg aggttcttgt tcaaatacat caagtccagc accagctatc
     6241 catttattct caagtgcttt gattaaagcc tcttcatcta ccgttgggcc tcgcccgtta
     6301 ttaataaata gggccgtcga tttcatggat ttgaattgag actcactcat tagatggaaa
     6361 gcatcctctg tggagggagc atgcattgaa actatatctg acatactcaa catctcttcc
     6421 aaggtgctaa taggttgtac cccgtattct gtcatagata gttcttcaat atagggatca
     6481 aaggcaatta atcttagacc aaaagctttg gctctaatcg atactgcttt agctacatgt
     6541 ccaaaggcta taaaacctaa ggtttgcccc cacaatcgtg gaaattctga aagaagaggt
     6601 cggccttctg accacctatt ttctcgtacc atttgatcca taatatttag tcttcgatat
     6661 gaagcaagaa gaagcatcat ggtgtgatca gccacttctt cgataaaggt gtccggaaca
     6721 tttgtgactg gtatgccctt cgccgttgct gcatttacat caacagtatc tgcaccgaca
     6781 ctgccaaggg agataacttt gcaattctcc aaagcatcta tgattttctt ggttatcggt
     6841 cgacctctag cgattacggc gtctgcgtct ttggcagaag ctataaactc gtcctctgtt
     6901 gccgcatcga tttctattat ttctgcatca atggagtcaa gtgattcctt ttctagcaaa
     6961 tattgaccat ctgccaaatc aaaagtaacg ccagctggtt tttgtgttac taccttgtac
     7021 ttagccatac tctttctctc ccctatcatt cattaaatcg ctgactacac ccgtcaacaa
     7081 agggcgtaca ggatatgaaa tatttgaaga ggtgtccagc aatacgtcta aaaaccgtct
     7141 gaacaatggg catgttttga tggttttgag cagtttttcc atcagagtat ggcaattgga
     7201 tttacgggag aatcggtagt atttgttact ttcacaggtg ctatgtttat caaaaattcc
     7261 cacttgctac tcgctgcaca ttattttgct agatcatcta ggttggcatt atctaaaatc
     7321 cacatcccca tagctactat ttacacttgt tgtatgggct gtacaacgtt agggtaaact
     7381 gcctcgttga tcgtctgaac ctcccctgcc ccagttactc aatttttcga aataaaacaa
     7441 aaactctttt tcaataggaa tctcattctt cattctctcc tccactatct ctatttatct
     7501 cttattaatt aaaattatca atgcggtttt acaatactga catattcgca cagtcattag
     7561 tgtttattta gtgagctaag ctctataatg atcgctcatg caacatattg atttcacccc
     7621 cgtggcattt tgataacgaa gattcttgaa agccgcttcc tagctgccct tcaatataga
     7681 gattacaggg tcctatggac aggtaatatg agtgcgagcg ctgcagcctg ggctcttata
     7741 gtcgctcgag ggtggatcgt ttgggaaatg tctgagtcat cactttacgt aggactcgtc
     7801 acctttctag caatgattcc cagggtcata atccctccgt ttacgggata cctggctgat
     7861 cggtacgaaa gacgaaaggt aatggccact atatatggac tcaatctaac acacacatta
     7921 tttcttgcgg ggttattagc ttttggtcag atagaaatgt ggcacctcat gattctcgca
     7981 tttgtgaatg gtgctgcaag atcagcacaa atgcctgttg gccaagcctt agtcccaaat
     8041 ttagtaccca aggacaaatt actaaatgcc attgctttaa atcaagcgac tatgcacggg
     8101 tcaagacttc taggaccatt aaccatactc ccccttcttg aaagcacgcg tatagagtgg
     8161 gccttcctag catgttctat gttttacgtt gttagccttg ttcaatcctt aaggataaaa
     8221 acacctagca caggatcgat ggataacaaa aaagggttct tttctaactt tactcaaggc
     8281 ctaccgtacg tttatagaca tcctttgttg aaattaatag taatgatggc cttctttcat
     8341 tgtggtttca caatgtcatt cgaagctgtt ttacccgtct tgtcagtgga aagatttaat
     8401 gcaactgacg gagcagactt cagcctcatg atgatgtcta tcgggggtgg cgcgttaata
     8461 agtttgatat ggctatctgg ggttactagt gagacaacaa aaggtaaatt attccttaac
     8521 cttggggtaa taagtgggtt agccccaatc ctgttggcct tttccccaaa catatacttc
     8581 gcaataggga gcgctgctct aatgggagcc tcacaagcag gatatatgac cctcactcat
     8641 acaatgattc aattggtaac cgacgactct gtgagagggc gggttggagc agtatattct
     8701 gttcatattg gaggaataat ggcatccatg aatctggcaa atggggcaat ttctgatttt
     8761 gctttcctaa aagtcgacct atttaacgga ttgagagttc ttcttcccgc ggacactatg
     8821 ctcactatag gggggatttt attcatcgca acagttttta taagctggtt cataatttca
     8881 ttgcggaaaa tttatcagac tggcatcccc caatcctcat taattcaggc ttagaatttt
     8941 aagttggaat aaaatcaaga aataataact tcgagacctt gtgacacttt cattacttct
     9001 tcctttggta ttgcagaagg agacactatt tgacactgca aagaggccgc tgacactgca
     9061 aaccggcaag aaacatttgc atcccaacct ttactaattc catacaaaaa tcctgacgac
     9121 aacacagcac cagctccatt agcgtctaac aagtctatcg aaggtggccg gacagagaat
     9181 gattctgact tggatttcca aaaacaaccc tcttctgatc cagtaaagag cactcctttg
     9241 actcctagtc ttaataactc attacacgtt tcatacttat tgtgggtgtt attagtttgg
     9301 tcaggggtgc attgaaccca agtagctagt tctaccagct ccttgactga ttcagtgctt
     9361 tggggtcggt cttctatatt aatgagcacc ggaacatttt tttccacagc tttcttaatt
     9421 gcaggcaaat tgtatggata atcgtaccaa tcagcataag cacatttggc acccgaaatt
     9481 acagcagtat cggcaaactc caaagtagtc agtaaatctt ccctcctatc ccaaatatat
     9541 gtccgtcctc cggtagagtc tgatataaca atttcgtatg gagtttcaag gtttttgtcc
     9601 ttgacaaatc ctcccaccac tcccaatgcc tctaactcgt cgaacacatt atttcctgaa
     9661 acgtcatccc caagtttgtt acataccagg ccgctggaaa ggccccattt tgcaatacaa
     9721 caggcgacga tagcagcatc gtcagaaatg aagtctgcac ggctgatcca tttggtcccc
     9781 gtattccaat ctggaagttt ctcaactaca taaacttcag ccggagtcac catgccaata
     9841 caaacaacat caataggttc tttcctatgc ctattaatat tgattatatt ttttttcatt
     9901 tttcttcctg aggctccgat ttcaaggatg gaagattaac attatattta gaagtgctac
     9961 aacgcgctct cattaggtag taaaatttac tgaaacctga ccaactgttt gcaagaatgt
    10021 ttaccaacat gatagccata gtatttttat catttctagc aggaataata gggataaccg
    10081 cctttgtcac aatttactac gctattgaaa cgaggcggca aggttttaaa atattctggg
    10141 aaaaacttga atatttttcc tccattgtat ccattgaaaa agacaaaata actttccatc
    10201 ctcgtggcag agacgctact gatggcctgt taccttgtgt tggcttaatt ggtcgtgatg
    10261 gttcgatact cataggggga cccccttacc aagaaaatgg gatcataact agagagattg
    10321 tttcaaaaaa tggatctttg ggacaagggg ctaggataag aactagtcta ttcatatatg
    10381 aaaccaaccc taaaattgat cttggacttg actataccga tgtgtctttc gattctgaac
    10441 ttggaactct taccggatgg cacatcccaa ataatagcaa caactgggtc ataggtgttc
    10501 acggccatag atcttataga acagaatcta tgcgatttgt gtcaactttc cgtaaatctg
    10561 gcctgaatgt tctgttgtca gactatagga atgatcaaaa tgcaccgtta gacaaaggtg
    10621 gctaccacat gttcggccta accgaatggc gggatttgga aagtgctgtg aaattctgcc
    10681 aggaaaaggg tgccgaaaaa atatttctga tgggacacag catgggaggc gcaatagtag
    10741 tcaagtattt gttggaatct tctttaaaat cactaataaa cggggcaatt ttagaatctc
    10801 ctgctttgaa tcttaacaaa attatctcaa tgaaggcggc agaaatacca ttgttaccac
    10861 ggggagccga atatcctgta aaaaaattgg tctcttggat ggttaagttg aattgggaga
    10921 acctaaacta cttatcaaga agcgatgaat taacgactcc catcctgctg atacatagtg
    10981 agtccgatgt gacagtccct gtttctctga gtgatcaatt agcggaaaac aggtcggaca
    11041 tagttacgta cctccggctt aaaaaagctc accatgctgc agcctggaat accaataagg
    11101 aaattgttga atcttctatg gaagcatttt taaacaaaaa tataagttct actgatgatt
    11161 gaatgaagta acaaattatg agtttgaaga tttcctccaa gccaaggata caaggaaccc
    11221 gcaaaacctc ttcagcagca attgccatca ccattacaaa aagttatttt cttaaacctg
    11281 tatgttatat tgcctgaatc aaccgtctat gaattgaggt aagcatggcc aagttaaaag
    11341 gaacaggtat gctcgctgtt tggagcgagg tagcctcgga gcatgaagaa gaattcaatg
    11401 agtggtataa ccaggaacac atatcggaaa gattgtcggt tcccggagtc ttaagtgcgg
    11461 ctcgttacga agctgtgaaa agcggcccaa aacacctagc ggtatacgag ctggaacatt
    11521 cttcagttat gaaatctcca gaatatgttg agttgaaaaa caatccgaca gattggtcaa
    11581 aacgaatgtc accagatgtt ataggaacaa aatacatccg aaatgtatat caaatgatat
    11641 tcccaacaga actgacagag gaagtagttt caagccctat ggctccagct ttacaaattg
    11701 gaaggatgaa tattcctagc aacacagaag atgaatggaa caattggtac aacaaaattt
    11761 acgtgccgaa ttatgaaaca gtcgacggcg taatacgagg ccgtcgctat cagactgtcg
    11821 aaggctcacc taaatacatg actttttacg aattagattc cgccaatgct tcgcagactg
    11881 aggagtggca aaaacaacaa actgcccatc ctgacaatga aaggatgcgg caagaaatgc
    11941 agcatgagtc agattcccct ggtatttggg ccaaaacttt tgaactttag atataaacgt
    12001 ttaggatcac caagtaaatc ccaaggacac ctttaacccg atggaatgga tgtggttttc
    12061 attagcgagt gcctttacct ttgcacttgt gtcagtccta gacaaattac tgatttcaaa
    12121 gcatgttgat aacgctaagg tattcatagt cactgtggga gtggcacaaa tctgccttgg
    12181 tcttattgtt attcccatat ccgcattttc gggcctaaca ttaagcaccc tgaccgttgt
    12241 aattttttct ggtatatcct ctgggatgta cctcgttatt atgttccaaa taatggaatc
    12301 gcaggacgtt tcacgggtcg tacccgtggt ctccacctac cctgtctttg ttgccgcatt
    12361 ggcttttttc atattgggag aacaggtcac catatattcc ctagcctgca tattaattac
    12421 agtctttggg gcagccttag tatcgttgtc accctcgggg aaaaaggcgt tagccaaatc
    12481 tgacgttacg gcaatgctgc tcctattcct agcaagtatt cttttcggat taagtcaatt
    12541 cctagctaag acaattgcag atgatgttga tatgtggaca cagtatatgg ttcgcggagt
    12601 atccggaggg cttacctgtt tttcattaat cctgctgcct ggagtagtga aaggtgtcac
    12661 atctatcttt cagaaaccta aatctttgtt tcttattggg tttactgaag gatttctagt
    12721 tttcttcgca atattgtttt tcttcctggc tatatatgca ggagaaattt acatggtgtc
    12781 gacggttatg gccagcaggc ctatctttgt ctttggtata ggccttatcc tcagcctacc
    12841 aatgataaaa ctattaaatg aaccattaaa tgggagagtt ctggctgtca gagcaacagg
    12901 aaccttctta acggtgttag gggttgctgg agtctcattt ctataacctt ctgaccactg
    12961 tatttgattc tttctaacct aatcttaaac acagattaaa agtgggtagc gccttaaaaa
    13021 ccctaccctt catatttatt atgtctgtaa aaaggttcta gttaacccaa aaactattta
    13081 ttaaaatcaa tggagtggca aaaatcaaac cagtgcgttt tcaccggtaa tatgtcgccc
    13141 aattatcaag gtgtgtacat ctctcgtacc ttcataagtg tcgaccgttt ctagattcaa
    13201 catatgtctt atgacaccgt agtccaatgt gattccgctg ccacccaaca cttcccttgc
    13261 acttctggct gccttcaagg cagaacgaac attctctctt tttgcaacgg aaacgtggtt
    13321 ataatccatt gttcctgcat ctttcatttc ccctaacctc caggccaata acagtccctg
    13381 agtatgatct gtaaccatat ctaccaattt atcttgaaca agttgcctgg aggcaatagg
    13441 tttgccaaaa gtgcttcttg ctttagagaa tctaagagca tcttcataaa cactttcaag
    13501 tgcaccaaga gcaccccaag cgatgccata gcgcgcctga gttaggcagg ataaaggagc
    13561 tgataaaccg ttcgattctg gtagaatact ggtagccggc actctcacat cttccagcac
    13621 cagctcactg gtaactgatg ctcgcatgct catcttgtgc tctattttgt tggccgtgaa
    13681 tccaggtgtg tctgtaggaa tcaaaaatcc tctcaccgta tctgtctcat ctttggccca
    13741 aacgagagcc acatcggcaa tatttccgtt ggtaatccac atttttgtac cgtttaatat
    13801 gtaggattca ccatctttct tggcgtttgt cttcattgcc cccgggtctg aaccaccatc
    13861 atgctcggta agtccaaaac atccaatggt attgccttta gccatttcgg gtaaatattt
    13921 tttcttttgg tcttcagaac catatctata tatgggatac ataaccaagg cactctgtac
    13981 actggcaaag cttctgaaac ctgaatctat tctttcgagc tcatacatga tgaggccata
    14041 cgatttgtta ttgactccag ggcaaccata ctccgcaggt agatttgatc ccaagaatcc
    14101 catttccccg aactcaggaa ccaaatgttt gggaaattca ccaaactccc accaatcctt
    14161 tacacctggc attacctcgc tgtccagata atccctagca gaattttgaa tctgcctatc
    14221 ttccgtggaa agaagttcag ttacgttgta gtaatctagc atttagaatc ctcaattatt
    14281 cctgtttttc actaacaggg tagatataaa atttttatga atatagtatg tcgttgtgtc
    14341 aaattaattt ccaccctaag tattgaattt gtcgaagaac ccataattga acaagcttcc
    14401 taaatctgat cgcaaaataa ttagagcctg gtgtatgtat gactgggcga attctggtta
    14461 cgctacaagt gggttagccg catttttccc tgtgtattac gttctcctgt tcaaaaatgc
    14521 cctaggggaa gaagctgttc tatgggggat aaatttcaca ggaagctcat cttggagtgt
    14581 agggttagcc atttccacct tgatagtagc aattacaagt ccaatcctag gagtgattgc
    14641 tgacaggatt ccgatcaaaa aaactttgtt atggatctac accgtagcag gctctctatt
    14701 cgccgtgctc gggtttttct ccgcatacac gtctcatccg tgggcttggc tattaggaac
    14761 attcatattg ggtaatattg ggttcgctgg tgggctagta atttacaatt catttcttcc
    14821 tcatatagcc ccgagaaact tattggatga aatcagcagt cgcggatatg cctttggcta
    14881 tattggcggt ggcctactct tattattcca tgtgattgtt ctggtattga cttctcaaag
    14941 tgatcacact gatttgatcc tgaggctatg tatcgcttcg gtcggcttat gg
//
LOCUS       DASCNS010000062         4711 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000527257, whole genome shotgun sequence.
ACCESSION   DASCNS010000062 DASCNS010000000
VERSION     DASCNS010000062.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000527257"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="QF838_02460"
     CDS             <1..1323
                     /locus_tag="QF838_02460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HJO59587.1"
                     /translation="CIMVRDNPSLGFKGTVKVDDFSLFFKYVIILATGLVILSSTTYA
                     KRFDKHQGEYYALILLSATGMMLLVSAIELITIYIALELTALPTAALAAFIREKKSAE
                     SGIKFLILSAVSSAVLLYGMVLIYGFTGTTHLSEIGTVIANHLSASEKITENGILLFS
                     VILITAGFGFKISAVPFQMWAPDVYEGAPTPITGFLSVASKAAGFAIILRILYAVFEN
                     EALSSDWASMMAVLSAISMSVGNLIAIKQNNIKRMLAYSTIAQAGYIMVGLAAMAGNT
                     TLYTSGPVGILVYLAGYTFTNLAAFAVITAVSEDSGDDSVDSFAGLIKKSPTLAVIMT
                     VSMISLIGIPPTIGFMAKIYLFGAAVETNLTWLAIVAVINSVVSAYYYLRVVKTMFID
                     SPDERQEIHPKFGVLSAAVIAISGTVFFGFFPKPIIELARDAITTIIG"
     gene            1394..2293
                     /locus_tag="QF838_02465"
     CDS             1394..2293
                     /locus_tag="QF838_02465"
                     /inference="COORDINATES: protein motif:HMM:NF039482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HJO59588.1"
                     /translation="MSKNKLIALIYNSQMPATEVKAKEIIDSLGIENVSWLSSAGNLS
                     EYSENLKETSLVIVVGGDGTILRSVRVISPYELPVVGIKMGKVGFIAELAPEEAISAL
                     PKYINYQSEKGNGNGIRTEKRMMLQADIIPTRGGDPRLSVHALNDITVGSRKVSRLVE
                     LETSVNHVHLTNYRADAVIVSTATGSTGYALSAGGPIVFPEAQMMILQPVAAHTGLRD
                     ALVLHPESTVELQASKNYLASISADGFVDSDVDPGEKVIVKKSPYNARFLRSEKPDFF
                     YTALNMRLGLAYRSQRPPEDPED"
     gene            2301..2837
                     /locus_tag="QF838_02470"
     CDS             2301..2837
                     /locus_tag="QF838_02470"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HJO59589.1"
                     /translation="MENIDLDRTIDSKKIYEGSIINVKIDNVLLKTGAKASREVVEHR
                     PAVAIVPMDSENNVLLVRQYRHPAKSDLLEVPAGVIEIGEEPEIAAIRELREEVGYTS
                     RDLRLLFGFWTSPGFTDEYIYGYLAKDLVEGKLPRDFDEDISVEKIPVRRIQKLIRLG
                     EIQDAKSIALLMSLETTI"
     gene            2860..4218
                     /locus_tag="QF838_02475"
     CDS             2860..4218
                     /locus_tag="QF838_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013696.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HJO59590.1"
                     /translation="MTNLDQMLRQIDDLEGDLIALEQALVRIPSVNTGFMPTGDETPV
                     CKYISDWLSKAGIKSEILESSPNRGNIIANIDGTSGKLGLLFMSHTDVVPVEDEDKWT
                     YPPFSAEIHQGRIYGRGASDCKGLLSAQLMAMKVLKQNGVVLKDGLSLISGADEEHGG
                     RYGFGWLAENYPEKLQAPFAVNEGGGSPIEAAGSLTYVLGVGEKGRLQIEIDVKGVSS
                     HASVPWQGTNALFRLSQVLNRIEEYEAERDTSTSLFEHLSTFAIEHKPSSDNVDEIIK
                     DQEEENPRFASMLRALSRMTITPTMINGGIKSNSVPEQIRLTCDVRTLPHQSDDYLRD
                     ELNKILEGIPGVEYSIDYMAVPNSSPFETELMTSLHEATKKAVDREDVQFVPAISTGF
                     TDSRFTRPLGVTTYGFSGSHPDDDPMLSKAHGTDESIGIKSLVSGAKVMLHLAYDMLA
                     EK"
     gene            complement(4236..>4711)
                     /locus_tag="QF838_02480"
     CDS             complement(4236..>4711)
                     /locus_tag="QF838_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO59591.1"
                     /translation="IGWFEEPLDPINYSEGLARIASEIAIPLAGGESGYGEGFFDSLA
                     QLGSVQTIMPDIKYCGGVQEAVRIANNAHNNGINFSPHCPSGPISLLASAHVCASLCK
                     KVLLEHAIEEINYRNELISPREIVRNGRIEMPTGIGLGAALSKTALKKYGEYFSF"
BASE COUNT         1472 a          979 c         1017 g         1243 t
ORIGIN      
        1 tgtattatgg tgcgagataa tccgagcctt ggattcaagg gcaccgtcaa ggtagatgat
       61 ttcagtctat tcttcaaata cgtcattatc ctcgcgacag ggctagtaat cctttcatcc
      121 acgacctatg cgaaaagatt cgacaaacat caaggcgaat actacgcact catcctttta
      181 tcagccaccg gaatgatgtt actagtgtca gccatagaat tgatcactat atacattgcc
      241 cttgaattga ctgccctgcc cacagcagct ctggcagcat ttataaggga gaagaaatca
      301 gctgaatccg ggattaaatt cctcatattg agtgcagtca gctctgcagt ccttctctat
      361 gggatggtcc taatatatgg attcactggc actactcatt tatctgaaat aggaacagta
      421 atagcaaatc acctttccgc ctcagaaaaa ataacagaga acggaattct tctatttagt
      481 gtaattttga taaccgcagg attcggattc aaaatttctg cggtcccatt tcaaatgtgg
      541 gctccagatg tttatgaagg ggcccccacc ccaatcacgg gatttctttc tgtagccagt
      601 aaggcggcgg ggttcgccat aattctcaga atcctatatg cggtttttga aaatgaggcc
      661 ttatcttcgg actgggctag tatgatggcg gttttaagcg cgatttcaat gtctgttgga
      721 aacctaatag ctataaaaca aaacaacatc aagcgaatgt tagcctatag caccatagct
      781 caggcaggct acataatggt gggcttggcg gcgatggccg gcaacacaac actatacacc
      841 tcaggacccg ttggaatcct tgtatatcta gccggataca cgtttactaa cctcgcagcg
      901 tttgcagtaa tcacggctgt ctcagaagat tctggtgatg attcagtcga ctctttcgct
      961 ggccttataa agaagtctcc tactcttgct gtaataatga ctgtttcaat gatatccctt
     1021 attgggatac cccctactat aggatttatg gcaaaaatct atttatttgg agcggctgtt
     1081 gaaaccaacc ttacctggct ggctatagtc gcggtcatta acagcgttgt atcggcctat
     1141 tattacctaa gagtagttaa gactatgttc atagatagtc cagatgagag acaagaaata
     1201 cacccgaaat tcggagttct ctctgctgcg gtcatagcta tttcaggtac tgtgtttttt
     1261 ggttttttcc cgaaacccat tatcgaatta gcccgtgatg ctataaccac cataatcggt
     1321 tagcccaaat aagttagtat ttatactata gatacttaaa aaccgatcaa aacgcatcta
     1381 tctggttgtg ttcatgtcaa aaaataaatt gattgcctta atttacaact ctcaaatgcc
     1441 tgccacagag gtcaaggcta aagaaatcat cgattcatta ggaatagaaa atgtttcatg
     1501 gttaagttca gcaggaaatt tatctgagta ctctgaaaac ctaaaagaaa cgagcctggt
     1561 tattgtggta ggaggtgacg ggacaatcct aagatctgta agagtaataa gtccttatga
     1621 acttcctgta gttggcataa aaatggggaa ggtggggttc atagcggaat tagcacctga
     1681 agaggctatt tcagcacttc ccaaatacat aaattaccaa tcggaaaaag gcaacggtaa
     1741 cggcataaga actgaaaaac gaatgatgct acaagctgac ataatcccca caagaggtgg
     1801 cgatcctcga ctatccgtac atgctctaaa cgacatcaca gttggaagta gaaaagtctc
     1861 cagactggta gaacttgaaa cttctgtaaa ccatgtccat ttaaccaact acagagctga
     1921 cgctgttata gtatcgactg cgactggaag tacaggatac gccctttcag ctggagggcc
     1981 catagttttc cctgaagcgc aaatgatgat attacagcca gtagcagcac atacaggtct
     2041 tcgagatgct ttagtactcc atccggaatc aaccgttgag cttcaggctt caaagaatta
     2101 tttggcatct atcagcgcag acggatttgt cgattcagac gtggatcctg gagaaaaagt
     2161 tatagtaaag aaaagcccct ataatgccag attcttacgt tcagaaaagc cagatttctt
     2221 ttataccgcg ttgaatatgc gacttggcct cgcataccga tcacaacgcc ctccggaaga
     2281 tccagaagat taggcatagt atggaaaata ttgatcttga ccgaactata gacagcaaga
     2341 agatttacga aggtagcatc ataaatgtga aaattgataa tgtgctgcta aaaaccggag
     2401 ccaaagcctc tagagaggtc gtcgaacaca gaccagcggt cgctattgtt ccgatggatt
     2461 cggaaaataa tgtgttactt gtccgccaat atagacaccc ggcaaaaagc gacctattag
     2521 aagtgccagc tggagttata gaaattggag aagaaccgga aatagcagcc atccgagagc
     2581 tccgagaaga agtaggatac acttcaagag atcttaggct attatttgga ttctggacga
     2641 gtcctggctt tacggatgag tacatttatg ggtatttagc taaagattta gtggaaggaa
     2701 aactaccccg agatttcgac gaagatattt cagtagagaa aatacctgtt agaagaatac
     2761 aaaaacttat aaggctggga gaaatacagg acgctaagag catcgcactt ttaatgtctc
     2821 tagaaacaac aatataaaaa attacgtgta ggtgagaaaa tgacaaacct tgatcaaatg
     2881 cttcgacaaa tcgatgactt ggaaggagat cttatagcct tagaacaggc tttagtccga
     2941 attccttcag ttaatacagg gtttatgcca acaggcgacg aaactccagt ctgcaaatac
     3001 atatcagact ggttatctaa agcaggaatt aaatctgaaa tcctggaatc atcaccaaac
     3061 agaggaaata ttattgccaa catcgatggg acttctggca agcttggcct tttattcatg
     3121 tcacacaccg atgttgtccc cgtggaagat gaggataaat ggacctatcc accatttagt
     3181 gcagaaatac atcaaggtcg aatctacggc cggggtgcct cagattgcaa aggcctcctt
     3241 tcagcacaat tgatggccat gaaggtattg aagcaaaatg gagtggtttt aaaagacgga
     3301 ttatccttga tttccggtgc tgacgaagaa catggaggaa gatatggctt tggatggctg
     3361 gcagaaaact acccagaaaa attacaggcc ccgttcgctg ttaacgaggg cgggggatcc
     3421 cccattgagg ctgctggatc attaacgtat gttttagggg taggagaaaa aggccgatta
     3481 caaatcgaga tagatgtgaa aggagtcagt tcacatgcat cggttccatg gcagggaacc
     3541 aacgccctct tcaggttgtc tcaagtactt aatagaatag aagaatacga ggcagagaga
     3601 gatacatcca cgagtttgtt tgaacattta tccacattcg cgattgaaca caaaccatca
     3661 tccgataacg tagatgaaat tattaaagat caagaagaag agaatccaag atttgcatcc
     3721 atgcttcggg ctttatcaag aatgacaata actccaacta tgattaatgg aggaattaaa
     3781 tcaaatagtg tacctgaaca aattaggctc acttgtgatg tgagaacgtt accccatcag
     3841 agtgatgatt acctcagaga tgaattgaat aaaatactag aaggaatccc tggagtggaa
     3901 tattccatag attatatggc tgttcctaat tcatccccct ttgaaactga gctcatgacc
     3961 agcttgcatg aggctactaa aaaagctgtg gaccgagagg acgtacagtt tgttccagct
     4021 attagtactg gattcacaga ttctaggttt acgagacctc tgggggtaac tacttacgga
     4081 ttttcaggat cgcacccaga cgatgaccca atgctcagta aagcccatgg gacagatgaa
     4141 tctataggaa tcaaaagtct agtcagtgga gcaaaagtca tgctgcatct ggcatatgac
     4201 atgctggcag aaaaataacc caattttcac tgtcgctaga aggaaaaata ttccccatat
     4261 tttttaaggg ccgtctttga cagggctgcg cctaatccga tacctgtcgg catctcaata
     4321 cgaccatttc tcacaatctc ccgaggagat atcaattcat tcctatagtt aatttcttca
     4381 attgcgtgtt ccagtaatac ctttttacat aatgacgcac aaacgtgtgc tgaggctaga
     4441 agagatattg ggccagacgg acaatggggc gaaaaattta taccgttatt atgagcatta
     4501 ttggcgattc gaacagcttc ctgaactcca ccacagtatt taatatctgg catgatagtt
     4561 tggaccgatc cgagttgggc gagagaatca aaaaatccct ctccatatcc agattcccct
     4621 cctgctaatg gtatagcgat ttcactagca attctggcta aaccttcaga ataattaata
     4681 ggatctaaag gttcttcaaa ccacccaatc c
//
LOCUS       DASCNS010000063        16790 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000532824, whole genome shotgun sequence.
ACCESSION   DASCNS010000063 DASCNS010000000
VERSION     DASCNS010000063.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 16790)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16790)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16790
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000532824"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            104..1012
                     /locus_tag="QF838_02485"
     CDS             104..1012
                     /locus_tag="QF838_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO59592.1"
                     /translation="MFGAWPIGGGMGRVDPQEAVKTIHAAINAGIAFIDTAEGYQTSE
                     SVLGQALKSRRDSVILATKLSGPDHSASHIRNALENSLSALNTEYIDLYQLHTPQPKW
                     PIENTMEILNDLKEEGKIRHIGLSNFTSGQTDEAMGYGPIVSSQPRYNLLFRQEDPTL
                     AFCEKNEIGVIPHSVLAKGLLGGSYKPGHIFATDDERRLFNFFQGDLFETIYKVTQNL
                     EEWSKNNGRDLIQLAIAWTLANSAITSAIVGMKSVAQVENAAKAATWKLTSTDLSEIE
                     SIIGNLRPAWIKAQTPEDIPYKYGSY"
     gene            complement(1019..2389)
                     /locus_tag="QF838_02490"
     CDS             complement(1019..2389)
                     /locus_tag="QF838_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO59593.1"
                     /translation="MQVNRILIKDAAYVITMDEKRRIISGGSILIENDRIAAVDKTID
                     VSQVDVDTVIEAKDMVITPGFINGHCHISYAHATRGIFPDDLGDDYLVNVFRLQDSMT
                     ADEELWTSLLAITELLSYGTTTIMDPGSTKSVDVCLDAYERSGIRMITGVQLTDTGNP
                     YRMTVYSHDQALRVTESFISQYNGALDGRLYAWAMPFSPEYVAGELLGELKVLSDQFN
                     VGCTLHVNKAGSSMPDGIDEPPIIALERYGFVGSNVTLAHCVGLSDEEINCLASTNTA
                     LITCPTNVVKNGGGYGQNFPLAELIKAKVRVGIGTDAGNNSNLLETNRAMYLAAVLIK
                     DATGDPAQIPAEKALELATIDGAKALGLESQIGSLEVGKKADIVMYDTLRPEWQSLFN
                     PVNSLVYNADGRSVKTVLVDGRVLIEDYVPRFVDTEKLIRRVQAFGSDMMKRNNVSFP
                     PKWPMI"
     gene            complement(2427..3428)
                     /locus_tag="QF838_02495"
     CDS             complement(2427..3428)
                     /locus_tag="QF838_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59594.1"
                     /translation="MEFGVQVNCYETDWSEISKTILAMEKGNWNSLWFADHFLPPHAL
                     SSKDDPVAAEKGNAFEGFTILAAAAGMTRRLRLGHLVLGNTYRNPGLVAKMSTTLDQV
                     SEGRFTLSLGAAWYEREHLAYGWPFPSMKERSDRFEEACELIRLLLTSKIPVDYRGKY
                     YQLEQAPMSPGCYQEPHLPILVGGTGEKRTLLTLAKYGDVFNLDGWSGRGMSMDLYRH
                     KVSVLESHCEKVGRDPDEIKKTLLMPIKITNDSKEARNFVRKLGYRSSDPAHGNYGGK
                     LVEAEESGSVAGSLDYVIERIEEFANEGVTEIMFGGMETGDVDTLEMLDDEIVSHFL"
     gene            3616..4233
                     /locus_tag="QF838_02500"
     CDS             3616..4233
                     /locus_tag="QF838_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59595.1"
                     /translation="MTSALIEIRRVPLPGKEFILSEGVKKSLEVTGKSGIITVTSASS
                     HRESRDVTTAITGLTLDEITELESSILNSPEAQSRLTALEELSVKVTYQTLVILAPPE
                     NTDVAKAQYINRRYLKAKRGQSQLLIEALLEWRDKFNRKPMVTRPLASDLDIVRITSP
                     IETLEELAGNISTINTDPDHKKNRDAVSELTASGFQTNNRIIHRV"
     gene            4282..5085
                     /locus_tag="QF838_02505"
     CDS             4282..5085
                     /locus_tag="QF838_02505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59596.1"
                     /translation="MARLSDKVAVITGGAGGIGKAAGKLFAEEGAHVVLVDLNESALK
                     DVVQSIGDENVSYVAADTTDPGQVQRFVNSTVERHGGIDIFIANAGIEGEVNSIIDTP
                     VEMFDKVIEVNVKGVWLGLKYVMPVMKERAGGSIVITSSTAGVRGSAGMAPYSTSKHA
                     VIGLMRTAAMEGSSMGIRVNTVNPAPIQTRMMRSIEEQRVAEGKQGFNTVEEAQKAVA
                     AGNPLRRYGEPEEVGKLMMFLASDESSFCNGGVYMVDGGTSAGQNSFGR"
     gene            5145..6317
                     /locus_tag="QF838_02510"
     CDS             5145..6317
                     /locus_tag="QF838_02510"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59597.1"
                     /translation="MRDFRLLASASFFESVTRGENVVLGWVILELTDSPFMVGLAMGI
                     RHAPAFFLGLTAGTISDLVDRRTLIRALMATATLVAFLMGLLLASGKSELWHLLIIPA
                     IGGTCHMMINTINRSFVFDLVGAENGLLGMSYLVLAMRSGGLVGALVVGFVLGKWGAG
                     TGYFAISIGCGCALLILALIKSPGQSAPSRKGSLSNGFYEFWQELKRNRTLSALVFIV
                     MSVELLGFTSQALMPSIARDIWDMGPEGLGLLNAFSSAGGIAAIAIVSILGQKGRQGI
                     KFIIVLHVFGIALLLLGLAPTVYIAIIAIFVMSGMMALSDLFSQTLLQRLVPNDLRGR
                     VMGAWTTAVGTSPLGNLEIGALASILGVTAALSLHGSALILLSIVTMLTFRKLREV"
     gene            6406..7593
                     /locus_tag="QF838_02515"
     CDS             6406..7593
                     /locus_tag="QF838_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO59598.1"
                     /translation="MKIKNIRAVQVNIPKSPPTSKPRRPNWNNTSARALPINKYPEFT
                     TAHGQMPGANTNESIWVQVIAEDGTWGLGQTSFGEITASIVDYHFAPLLEGRECFALE
                     FLNDLMWRSTQRFGAGGIATVAQSGIDLALWDLKGKLMQQPVYSLLGGPCREKSLCYV
                     TSDDLDWSIELGFKHFKVSNPAHYDMGIEGLNLVEEKISAARETIGNNAELMYNPVMS
                     FNVEFAVRMAERLRPYGLRWLEEPLIPTDLEGHIELRKAINWVPLATGEDHHGRWTFR
                     QLVENRAVDVLQPDLKWCGGLTEALKIYAIGEAAGLITIPHAAAASPWGQHFAISMPE
                     SPMAEFWMGSDPGIPLEEVCPIPGMPMPKDGYIKPSDAPGFGMEIKDEWIIPWNHSAI
                     SGG"
     gene            7696..8640
                     /locus_tag="QF838_02520"
     CDS             7696..8640
                     /locus_tag="QF838_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016115.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HJO59599.1"
                     /translation="MGLKERTPTTVQEYLDYKTKFSNWGRWGDDDQFGTLNHINPSLV
                     KEAAGLIKTGETVSCANPLATQSVIPDPHRNPKPADHNMSVHERGSGDYIGVSYHGFV
                     NTHIDALCHFFTKSVEEGGRLYNDKDPALVTESGATSHSIENWRDGIVTRGVLYDIPK
                     FRGTDFVEFDKPVEGWELEDWAKSQGITPQRGDAVFIRSGYRSFWEAHPDLEMTFPPN
                     TPGNAPSIIEFLYTTDASLLGWDLQEAGHREHDYPARIAVHEVIIPHMGMPILDNADF
                     DRLANTCEELGEYTFHLTIAPLVVEGGTGSPVNPIATF"
     gene            complement(8674..9702)
                     /locus_tag="QF838_02525"
     CDS             complement(8674..9702)
                     /locus_tag="QF838_02525"
                     /inference="COORDINATES: protein motif:HMM:NF028222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO59600.1"
                     /translation="MEGLFFTGDSVVEAISIDKMAPASGEVLLQMKASGVCGSDLKNF
                     GASKSEKPKLSELKVPGHEPVGVVVELGTNVGGLSIGDRVMMHHYTGCLKCSMCLIGY
                     TQMCLVAHEVYGSTAHGGHQEYMCVPAYTCIPIPEGLDFVSAAACSCGTGTAFHAVKR
                     LNPTGLDTVAIFGQGPVGLSATMFAAQLGARVIAVDISSYRLNLAKELGASEVIDSSS
                     HNAEQVIRELTSGEGAECTLDATGIPEVRIQAVDSAKYWGKVCFVGEGNTTTFDVSAQ
                     IIHKQLTILGSWTFSQAGLSEVANFVIERRSPMDKLITHTFGLKEAQKAYDIFISGET
                     GKVILTMD"
     gene            complement(9771..10142)
                     /locus_tag="QF838_02530"
     CDS             complement(9771..10142)
                     /locus_tag="QF838_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59601.1"
                     /translation="MDTSKLEVGQSARIDVKVTTEMTTNRTGNTGDDVLSTPAMLGLM
                     EGACISQSDHLLDKNASTVGYAVDGLRHVARTDVGETVQISTSLTELDGAKGRLTYEI
                     EVTHNGNTVGKAIHKRAIISR"
     gene            10291..11178
                     /locus_tag="QF838_02535"
     CDS             10291..11178
                     /locus_tag="QF838_02535"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003902310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HJO59602.1"
                     /translation="MSNDKSFTEQYPKHHMTINGKSMAYIDHGEGDPIVLIHGNPTSS
                     YLWRNIIPHVAQLGRVVAPDLIGMGESAKLDNPGPHSYKFQEHTEYLYKLFRNLDLKN
                     IHFVIHDWGSVLGFYWTFLNPEKVKSISYMEAITSPIESWDDWPEVARNIFQAFRSDA
                     GEELILEKNIFVERILAGDGRLSEKDLKIYLEPFKLAGEDRRPTLSWPREIPIAGEPE
                     KIVQLVQEYSDFLRVSDIPKLFINADPGSILVGRQREKARLWNNQQEVTVQGGHFIQE
                     ISPNEIGSHLANFIKSLRN"
     gene            complement(11168..12403)
                     /locus_tag="QF838_02540"
     CDS             complement(11168..12403)
                     /locus_tag="QF838_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59603.1"
                     /translation="MRHYEVKSTVWSNPRLWAVSLGETLAWAGLFYLFPASLIRWKLH
                     FDWSISELSGALMIALIVSGITGIGSGKLIDKGLGRALMSGGVVLGGVCLLFIPMVSS
                     LEEFYILWAVVGIAMGACLYDPCFAILTRHYSSEAKEPIVMVTLFAGFSITFSFILSA
                     LISSVFNWETSFYVFGVLLCFLAAPLFWIGISVDTKVKPLAAGIFSESKSLVSFFRGL
                     IVRPIFWGLSILFTTFALNHIMLISQILPLFESKGASSTSSVLIASAMGPMQVGGRVG
                     LLVVEKRLKREFNIVWVAIFSCLVLVCASGMLYYSETSLLILGVFVLFQGLSFGIINL
                     VKPVIVATLLGESNFGFISAIVGIGYILGFAIAPGVSGLISDLWGLDALIATSFLTAL
                     LGLATFSGVILKSRNVINF"
     gene            12536..13927
                     /locus_tag="QF838_02545"
     CDS             12536..13927
                     /locus_tag="QF838_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HJO59604.1"
                     /translation="MNDLIYGSATKIASEIRQKNVSCLEVIEMHLKRIEVVNPALNAV
                     VQLCSERALAEAKAADVKISKKENIGPLHGVPMTLKDSLDTAEVVSTGGTKGRQNFIP
                     DNDSTVTSRLRNAGAILLGKTNTPEFTLAGETDNLVYGRTNNPYNLTRIPGGSSGGAA
                     AIIASGGSPLDIGSDTGGSIRMPSHFCGITGIKPNSGRIPRTGHIVNHAMGAVDSLTQ
                     NGPMARYVEDLILTLPILCGRDWIDPAIIEMPLGDPYKVKIENLRVAFYTDNGIHEPS
                     QAIQDAVKSAAMDLGDLGATVEEDRPNALGMIPEINNALNGGDGREWVKRLMESANTK
                     EISPFLQKRINNAEPISTAEYTANLERLDQYRSHMLSFMKHYDVIISPTAPFTATDHG
                     ETFSNKNKNAFAYTSAYNMTGWPGTVVRYGTCEENLPIGVQIVSRPWREDVSLAIALE
                     LEKISGGWKNPNI"
     gene            13997..15169
                     /locus_tag="QF838_02550"
     CDS             13997..15169
                     /locus_tag="QF838_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO59605.1"
                     /translation="MRESPVITKIEAIHYSHTLEEVSRDYNTFNMVYKPGGKSKQSGT
                     LVRIHTDQGLTGEYPDVTGPTLAEIKMLSSYLIGKNALTRELIYNDLKRGSRHWAMLG
                     MGIVDICLWDIAGKFYGEPLFRLLGGDLQPLKAYASTLHGDENGGLSTAGDFEDFAHK
                     CLQMGYQAFKVHGWGLARDNINREIENVLNLGNKFEGKLDLLIDPACEIKNFGDALKL
                     GKACDEANFFWWEDPFQDNGVSQFAHRKLRQLVKTPLLQTEHIRLLEQHVDFIVADAT
                     DYVRAGAHEDGGITGAMKIAHASEGFGLDVELHGPGPVHRHIMSSIRNTNFYELGLVH
                     PNLPTTKAPVYLDYSDDLDAIDQEGNVYAPQGPGIGVNLDWDWINFHKTGEEIIAE"
     gene            15208..16419
                     /locus_tag="QF838_02555"
     CDS             15208..16419
                     /locus_tag="QF838_02555"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020053180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59606.1"
                     /translation="MSTTEGALAGVRVVTCSTAQAGTVPYMLMADLGAEVIKIEVPGT
                     GDGSRRAGEIKNGVSSFFETNNRGVKSLTLNLKAEEGKKILHELVKRADVFGQNFRPG
                     AAERNGFGYEELRKINPRLVYASVSAYGLEGPDANLPGTDAVGQALSGITEAFSIPGQ
                     SMRTGVASVADETTAILTFGGVLAALIRARETGKGQKVDTSLVGSAFRLMGWTMATTM
                     WKNQAPITGARINGSRNRAGIAACFNDINGKPLAIQLDARHWISALQILGFHNLMEEK
                     GLLDLGLAFDSENKKNEILDTLSSLFATNTREHWVTILREADMVAAPVNTMLEASNDP
                     NVVANGYVKEIFHPKVGENIKIHGSPWNFSETPSNPGFAPELGQHNDEILSSLGYSES
                     QIKELIDIEVI"
     gene            16452..>16790
                     /locus_tag="QF838_02560"
     CDS             16452..>16790
                     /locus_tag="QF838_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO59607.1"
                     /translation="MADIRKNRAKQKLKNGETVIAVACTDTDMVDFLGSSGAVDVIWI
                     EMEHGEATWSQISDISRACDLWGMTSLVRVNNNDPWLVGRALDRGAQAVVVPHVNTKE
                     EAKRLVQGGKY"
BASE COUNT         5302 a         3606 c         3558 g         4324 t
ORIGIN      
        1 gaagaaacga atgaatttgg actagggttg ccaaaaaccc taccgttata gaaaaaatgg
       61 aaacaagtgt attaggaaaa cctgatttaa aagtctccac ggtatgtttg gtgcctggcc
      121 aattggcggt ggcatgggaa gagtcgaccc ccaagaagct gttaagacta tccacgctgc
      181 aataaatgcc ggcattgcct ttatcgatac tgctgaggga tatcagacta gcgagtcggt
      241 tctcggacaa gctttaaaaa gccggcgaga ttcggtaata ttggcgacca aactctctgg
      301 tccggaccat tctgcatcac atattcgtaa cgcactagaa aacagtcttt ctgcattaaa
      361 caccgaatac atcgatcttt accaactgca cactccacaa cccaaatggc ctattgaaaa
      421 cactatggaa atcttgaatg atttgaagga agaagggaaa atccgccata ttggtctatc
      481 taatttcaca tcaggacaaa cagatgaagc gatgggatat ggtccgattg tcagctcaca
      541 acctcgatat aatctgctct ttcgacagga agaccctaca ttggcttttt gtgagaaaaa
      601 cgaaataggc gtcattcccc attcggtttt agccaaaggt ctattaggtg gcagttacaa
      661 accgggtcac attttcgcta cggatgacga aagaagattg ttcaattttt tccaaggtga
      721 tctttttgaa acgatatata aagtgacaca aaatttagaa gaatggtcca aaaataatgg
      781 aagagactta atacaattag caatcgcatg gaccttggct aattcagcca taacatcagc
      841 catagtagga atgaaatcag tagcccaagt tgaaaatgct gccaaggcgg caacatggaa
      901 acttacttcc acagacctct ctgagataga gtctattatc ggaaatctac ggccggcgtg
      961 gattaaagca caaaccccag aagatatccc ttacaaatat gggagctact gaaagcttct
     1021 aaatcatcgg ccactttgga ggaaaggaca cattattccg tttcatcata tctgacccga
     1081 atgcctgcac tcttcgtatt aacttttcag tgtcaacgaa tctaggtacg taatcctcta
     1141 ttagaacccg cccatccact aataccgttt tcacgcttct accgtcagca ttgtacacaa
     1201 gactattcac aggattgaaa agtgattgcc actcaggtct caatgtatcg tacatcacta
     1261 tatccgcctt cttaccaacc tcaagggacc cgatttgaga ttccaacccc aaggcctttg
     1321 ccccatcgat agtggctaat tccagagcct tttcggctgg aatttgagca ggatcaccag
     1381 ttgcgtcttt tataaggaca gctgctaaat acattgcccg gttagtttct aataaattgg
     1441 aattattccc agcatcagtt cctatgccaa ctcgcacttt agcttttatt aattcagcca
     1501 agggaaagtt ctgaccataa ccaccgccat tttttaccac attcgttgga catgtaatta
     1561 aagccgtatt ggtagatgca agacaattaa tttcctcatc agacaatcca acacaatgag
     1621 caagagtcac attacttcct acaaacccat atctttccaa ggcaataata ggaggttcat
     1681 caataccatc aggcatagag ctgccggcct tatttacgtg tagagtacaa ccgacgttaa
     1741 attggtccga caagaccttt aactcaccca acagttcccc tgcaacatat tctggagaaa
     1801 agggcatggc ccaagcatag agtcttccat caagtgcacc attgtattga gatataaatg
     1861 attctgtgac tcgcaaggct tgatcgtggg aatatactgt catccgatag gggtttcctg
     1921 tgtcggttaa ctgaacacca gtaatcattc gtattccaga tctttcatag gcatccagac
     1981 atacatcaac acttttggta ctccctgggt ccataatagt ggtggtgccg taactcaata
     2041 attcagtgat ggctaagagt gaagtccaca actcttcatc tgctgtcatt gaatcttgta
     2101 gacgaaatac attaacaaga taatcatctc caaggtcgtc tggaaatatc cctcgagtcg
     2161 catgtgcgta acttatatgg caatggccat ttatgaatcc aggtgttatt accatgtctt
     2221 tggcttcaat tactgtatca acatcgactt gagaaacatc aattgtttta tctacagctg
     2281 cgattcgatc attttcgatc aatatactgc caccactaat aatccgtctt ttctcgtcca
     2341 tggtaataac ataggccgca tcttttataa gaattctatt aacctgcata tccaatctcc
     2401 atagttacct caatagaacc ttttctctat aaaaagtggc taacaatctc atcgtccaac
     2461 atttccaggg tatcaacatc ccctgtttcc atacctccaa acatgatttc cgtcactcct
     2521 tcgttagcaa attcttcaat tctctcaatg acataatcca gacttcctgc tacagagcca
     2581 ctttcttccg cttcaaccaa tttacctccg taattgccat gtgcagggtc agatgagcgg
     2641 taacctaatt tccgaacaaa attcctcgct tctttggaat catttgtgat tttgataggc
     2701 atgagcaaag ttttctttat ttcgtctggg tctcgaccta ctttttcaca atggctttcc
     2761 aaaacagaga ccttatgcct atacagatcc atagacattc ccctacccga ccaaccgtcc
     2821 aagttaaaga catccccata tttagctaaa gtcagtaatg ttcttttttc cccagtacca
     2881 cccaccagta tcggtaaatg tggttcttga taacatccag gcgacatcgg agcctgttcc
     2941 aattgataat atttacccct gtaatcaaca gggattttcg atgtcaataa tagcctgatc
     3001 aactcacagg cttcttcaaa tctgtcagaa cgctccttca tggaaggaaa aggccaccca
     3061 taagccagat gttccctttc ataccaagct gcccccaaag ataacgtaaa tctgccttct
     3121 gagacctgat ccaaagtagt agacattttc gcgaccaaac caggatttct atatgtattg
     3181 cctaaaacta aatggccaag tctaagcctt ctagtcatac ctgccgctgc agccaagata
     3241 gtaaatcctt caaaggcatt tcctttttct gcagctactg gatcatcttt tgaacttaaa
     3301 gcatgcggcg gtaaaaaatg atcagcaaac cacagactat tccagttgcc tttctccatc
     3361 gccaaaattg ttttagaaat ttctgaccag tctgtttcgt aacaattaac ttgtaccccg
     3421 aactccatct tcatatcttc aatatacctt tacaccctaa agtgataatc cgttgcaatt
     3481 tatagattaa tgaatcaaat ccggcaactc aagcgagatt ctaatctagt atttatctct
     3541 tatttggtaa gaagtaaacg aattcattac aatcactctc aaattcatca aatttaataa
     3601 actaaggagg tctaaatgac ttcagcctta attgaaatcc gaagagtgcc attaccagga
     3661 aaggaattca tcctgtctga aggggtaaaa aaatcgctag aggtcaccgg gaaatcagga
     3721 atcattacgg tgacaagtgc ttcatctcat cgagaaagca gagatgtcac cactgctatt
     3781 accgggctaa ctttagatga aataacagag ttggaatcct ctatactcaa ttccccggaa
     3841 gctcaatcaa gattgacggc ccttgaggaa ctttcggtaa aagtaaccta ccagactctg
     3901 gtaattctgg cacctccaga aaatacagat gtagcaaaag ctcaatacat caaccgcagg
     3961 tatttgaaag cgaaaagagg tcagtcacaa cttttgatag aagcccttct ggaatggaga
     4021 gataagttca acaggaaacc gatggtaaca cgaccactgg caagtgattt agacattgta
     4081 cgaattactt ctccaataga aacattggaa gaacttgcag gtaatatatc aaccataaac
     4141 actgatcctg atcataagaa aaacagagat gctgtaagtg aacttaccgc aagcggattt
     4201 caaactaaca accgaatcat ccacagagtt taattattct gtagcaaaaa aaagaagtac
     4261 aacacaaacc agaggtgctt tatggcaaga ctttccgata aagttgcagt gataacaggt
     4321 ggtgctggag gtattggaaa agctgcaggg aaacttttcg ctgaggaagg cgcccatgta
     4381 gttctagttg atctgaatga atcggccctg aaagatgttg ttcaatccat tggagacgaa
     4441 aatgtgagct atgtcgcggc tgacaccaca gaccctggtc aggtacaacg atttgttaat
     4501 tccactgtag aacgccatgg tggaattgat atatttatcg ctaatgcggg aattgaaggc
     4561 gaagttaatt caataataga taccccggta gaaatgtttg acaaggtcat tgaagtgaac
     4621 gtcaaagggg tgtggctagg tttaaagtat gtaatgccgg tgatgaaaga aagagctgga
     4681 gggagtattg taatcacttc ctctacagcc ggtgttaggg gatcagcagg catggccccg
     4741 tattccacat caaaacacgc agtcatagga ctgatgcgta ctgctgccat ggaaggctca
     4801 tccatgggta ttcgtgtaaa cacagttaat ccagctccta tacaaacccg aatgatgcga
     4861 tcgattgaag aacaaagagt ggcagaaggg aaacaaggct tcaatacagt tgaagaagca
     4921 cagaaagccg tggccgcagg aaatccccta agacgatatg gagaaccaga ggaagtcggt
     4981 aaactaatga tgtttctagc cagcgatgaa agcagcttct gtaatggtgg cgtctacatg
     5041 gttgatgggg gcacatcggc aggtcaaaac tcctttggaa gataatttgt tcccatttaa
     5101 taactaactt gttaaataaa attaagaatt cgcccgtcct aaggttgcgg gacttcagac
     5161 tactagcttc tgcatctttc tttgagagtg ttactcgcgg tgaaaatgtc gttctaggtt
     5221 gggtgatcct agaattaact gactcaccgt tcatggtagg tctggccatg ggcatacgtc
     5281 atgccccagc attttttcta ggtcttacag ccggtactat ttcagatctt gttgacagaa
     5341 gaaccctcat ccgggcattg atggcaacag ctactttggt cgcattcttg atggggttac
     5401 ttttggcatc tggaaaatca gaattatggc atttactgat aattccagcg atcggaggta
     5461 cttgtcacat gatgatcaat actatcaatc gtagttttgt ttttgatttg gtgggggccg
     5521 aaaatggatt attaggcatg tcctaccttg tcctggccat gcgtagtggt ggactagtcg
     5581 gtgcattagt ggtcggtttc gtactgggca aatggggagc cggaaccgga tattttgcca
     5641 tatctattgg atgtggatgc gctcttttga tattggcctt gatcaaatca cctgggcaat
     5701 cggcgccttc aaggaaaggt agtctttcaa atggtttcta tgaattctgg caagagctaa
     5761 aacggaatcg caccctgtct gcattggttt ttattgtaat gtctgttgaa cttctaggtt
     5821 tcacctctca agctttaatg cccagtatag ccagagacat ctgggatatg ggccctgaag
     5881 gactaggtct ccttaacgca tttagctctg ctggaggtat tgcagctata gctatagttt
     5941 caatattagg acaaaagggc cgccagggaa tcaaatttat catcgttcta catgtatttg
     6001 ggattgctct tctccttctt ggactggccc caacagttta tatagccatc atagctatat
     6061 ttgtgatgag cggaatgatg gctttatctg acttattttc tcagacgttg ctccaaagac
     6121 tcgttcctaa tgatttaagg ggtagagtaa tgggagcgtg gacaacagcc gtgggtactt
     6181 ccccgttagg aaacttagaa atcggtgcct tggcatcaat attgggcgtt actgcagcgc
     6241 tgtccctaca tggaagcgct ttaattctat tgtccatagt aacgatgcta acatttcgaa
     6301 aactcaggga agtataaccc tatagaaaag acatgtatta tgcagcaaag acaatcagct
     6361 cgaacttagc aatttcgaat gcatacacat accgcggaga actgaatgaa aataaagaac
     6421 attagagctg tacaggtaaa tataccaaaa tcacctccta cttccaaacc aagaagacca
     6481 aactggaaca atacctctgc gagagctcta ccgataaata aatacccgga attcacaaca
     6541 gctcacggtc aaatgcccgg tgcgaacacc aacgaaagca tatgggtgca ggttattgca
     6601 gaagatggca cttggggcct gggtcaaaca agctttgggg aaataacggc atcaatcgtt
     6661 gattatcatt ttgcaccttt actggaagga agggagtgtt ttgctttaga gtttctaaac
     6721 gaccttatgt ggcgatctac tcaaagattt ggagctggag gaatagctac ggtagcccag
     6781 tcaggaatcg atcttgctct atgggatcta aaaggcaaat taatgcagca gccagtttac
     6841 agtctactag gagggccttg tagagaaaaa tcactctgct atgtgacatc cgacgattta
     6901 gattggtcaa tagagctggg attcaaacat tttaaggtct caaatccagc ccattatgac
     6961 atgggaatag aaggccttaa tttagtggaa gaaaaaatct ccgctgctag agaaaccata
     7021 ggtaataatg ctgaattaat gtataaccct gtcatgtctt ttaacgtcga atttgccgtg
     7081 agaatggccg aacgccttag accatacggg ttgagatggc tagaagaacc tttaattccg
     7141 acagatttag aaggacatat tgaacttcga aaggcgatca actgggtccc tctcgcgaca
     7201 ggagaagacc accatggaag gtggacattc aggcagttag tagaaaatag agcagttgat
     7261 gtacttcaac cagatctaaa atggtgcggt ggtttgacag aagccctaaa aatatatgcc
     7321 attggagaag ctgcgggtct gattactatt ccacatgccg ctgcggcatc cccatgggga
     7381 caacattttg ctatctctat gccagaatca cctatggcag aattttggat gggatcagat
     7441 cctggtatac cccttgaaga ggtttgccca ataccaggaa tgcccatgcc taaagatggc
     7501 tatatcaaac cctcagatgc acctggtttc ggtatggaaa taaaggacga atggataatc
     7561 ccttggaacc actctgcaat atcgggtggt tgatatttca aattcaagag aaaatataga
     7621 aaccctatga gttcgacaca attcgaatca acaaattaaa tttgattagg aaaaatataa
     7681 ataaagtgag atgacatggg ccttaaagaa cgaacaccaa ccacagtaca agaatatctg
     7741 gattacaaaa ctaaattcag caattggggc agatggggag atgatgatca gtttggaaca
     7801 cttaaccaca taaaccccag tttggtaaaa gaagctgctg gccttattaa aacaggggag
     7861 acagtttcat gtgcgaatcc gttggcaacc cagtctgtga tccccgatcc acatagaaac
     7921 cccaaaccag ccgatcacaa catgtcggtc catgagagag gttccggaga ttacattggc
     7981 gtctcctacc acgggtttgt aaacacacac atcgacgcgc tatgccattt ctttactaaa
     8041 agtgtagaag aaggaggacg tctctataac gataaagacc cagctcttgt aactgaatca
     8101 ggagccacaa gccattcaat agaaaactgg agagacggca tagtgacgag aggggtttta
     8161 tatgacatac caaaatttag aggcactgac tttgttgaat tcgataaacc agtggaaggt
     8221 tgggaactag aagattgggc aaaaagccaa ggaataaccc ctcaaagagg tgatgcagtt
     8281 tttatacggt ctggttatag atcattttgg gaggcacatc cagatttaga aatgacattc
     8341 cctcccaata ctcctggcaa tgcaccttcg ataattgaat ttctttatac tacagatgct
     8401 tcattattgg gctgggacct tcaagaagca ggacatagag agcacgacta tcctgcgaga
     8461 atagctgtgc acgaggtaat aatacctcac atgggaatgc caattctgga caatgcagat
     8521 tttgatcgat tagcaaacac ctgtgaagag ttaggcgaat ataccttcca tctaactata
     8581 gctcctctgg tagtggaagg aggcaccggg tcacccgtaa atccgatcgc tacgttttag
     8641 attacgaacc tttcagattc gtctatcaca gcgtcaatcc attgtaagaa taactttccc
     8701 tgtttcacct gaaataaaaa tatcatacgc cttttgagcc tctttgagcc caaatgtatg
     8761 ggtgattaat ttatccatag gagatctcct ttcgatgaca aagtttgcga cttcgctcag
     8821 accagcctgg ctaaatgtcc aagatcccaa tatagttaat tgcttatgaa ttatttgagc
     8881 actaacgtca aaagtagtgg tattcccttc tcctacaaaa catactttcc cccaatattt
     8941 agcactatca acagcctgaa ttcttacctc tggtattccg gtggcgtcta aggtgcattc
     9001 agcaccttct cctgaagtga gttctctaat aacttgttct gcattgtgtg aagaagaatc
     9061 tattacttcg ctagcaccta attctttagc taaattcaat ctgtatgaag atatatcgac
     9121 cgctataacc ctggcgccca gctgtgcagc aaacatagtc gccgacaggc ccacaggccc
     9181 ttgaccaaat atagctactg tatccaatcc cgtaggattc aaccttttta ctgcatgaaa
     9241 agccgtgccc gtaccacacg aacatgcagc tgcagaaaca aaatctaggc cctctggtat
     9301 gggaatacat gtataagctg gaacgcacat gtattcctga tgcccaccat gtgccgtcga
     9361 cccataaacc tcatgagcaa ccaaacacat ttgagtgtat ccgataagac acatagaaca
     9421 tttcaagcat cccgtatagt gatgcatcat taccctatct ccaatggaga ggccccccac
     9481 atttgtacca agttcaacca ccactccaac gggctcatga ccgggcacct ttagctcaga
     9541 cagtttcggt ttctcagatt ttgaggctcc aaaattcttt aaatcgctac cacacactcc
     9601 cgaagctttc atttgaagta acacttcccc agaggctgga gccattttgt cgatacttat
     9661 agcctccaca acagaatctc cagtaaaaaa caatccttcc ataactggtg ctcctttccc
     9721 gaaactcaat ggcatatatc tctattcggg gtagtgtgaa ataactttta tcatctagaa
     9781 attatcgccc ttttgtgaat cgctttaccc acagtattac cattatgagt gacctcgatt
     9841 tcataggtga gacgaccttt tgctccgtct aattcagtta gagaggtgct gatttgtact
     9901 gtttctccca catcggtcct cgcaacatgc ctgagcccat caaccgcata accgaccgtc
     9961 gatgcatttt tgtccagtaa atggtccgat tgacttatac aagcaccttc cattaggccc
    10021 agcattgccg gagtagacaa tacatcatcg cccgtgtttc cggtcctatt agtcgtcatt
    10081 tcagtagtaa cctttacatc tatacgagct gactgaccaa cttcaagttt actagtgtcc
    10141 aaagtatcct cctaaaacta aatttaatcg cgattatacc taagtgaaaa aaaggtggcg
    10201 taaaggaata tttaaggtta ttttgataat atcgagcaag tgaaattaaa tttactgaaa
    10261 gaaaattaga gaaaagtacc aaggaaaata atgtctaacg ataaatcgtt cacagaacaa
    10321 tatcctaaac atcatatgac tataaacggt aaatctatgg cttatattga ccatggagaa
    10381 ggcgatccca tagtcttgat ccatgggaat ccaacgtcct catatctttg gagaaatata
    10441 ataccccatg ttgcccagct tggaagagtc gtagcaccag acctaatcgg tatgggagaa
    10501 tcagccaaat tggacaatcc cgggcctcat agttacaaat ttcaagagca caccgaatac
    10561 ctatataaac tcttccggaa tttagacctc aagaacatac attttgtgat tcatgactgg
    10621 ggttctgtac ttggctttta ctggacattt ttgaatccgg aaaaagtcaa aagtatttcc
    10681 tacatggaag ccataaccag cccaatagaa agttgggatg actggccaga agtggcaaga
    10741 aatatttttc aagctttcag gtctgatgct ggcgaagagt tgatattaga aaaaaacatc
    10801 tttgtggaaa gaatactggc cggggatggg aggctttcgg agaaagatct caaaatttat
    10861 ttggagccat ttaagttagc aggagaggac aggaggccta ccttgagttg gcctcgagaa
    10921 ataccgattg caggagaacc tgaaaaaatc gtccaattag tacaagaata ttcagacttt
    10981 ttaagggtga gtgatatacc aaaactcttt ataaatgcgg atccagggtc gatattggtc
    11041 ggtaggcaaa gggaaaaggc cagactgtgg aataatcaac aagaagtaac agttcagggg
    11101 gggcatttca ttcaggaaat atcgccaaac gagataggta gtcacctagc aaactttatt
    11161 aaaagtctca gaaattaatt acattacgac tttttagtat tactcctgaa aatgtagcaa
    11221 gccctaacag tgccgttaag aacgaggtag caatcagagc gtcaagaccc cataaatccg
    11281 aaattaatcc ggacacccct ggtgcgatcg caaatcccaa aatataaccg atccctacga
    11341 tcgcagagat aaatccgaaa ttagactcac ccaagagtgt agcaacaatg accggcttga
    11401 caaggtttat tattccaaac gatagtccct gaaataaaac aaaaacacca agaataagaa
    11461 gactggtttc agagtaataa agcattccac ttgcacaaac taaaaccaaa cagctaaata
    11521 tagccaccca aacgatatta aactctctct ttaggcgctt ctcaactacc agtaacccga
    11581 cacgaccacc aacttgcatc ggtcccatag ctgaagcaat caaaacagag gaagtggaag
    11641 aagcgccctt gctttcaaat aatggcagta tctgactgat taacattatg tgatttagtg
    11701 caaaggttgt gaagagaata cttaatcccc agaaaattgg ccttacgatc aagcctcgaa
    11761 agaagctgac aagactcttc gattcagaaa aaatacctgc tgctaaaggc tttacctttg
    11821 tatccactga gatcccaatc cagaagagtg gtgctgccaa aaaacacagt agaacaccaa
    11881 acacataaaa actagtttcc caattaaaaa cactagaaat taaagcagac aaaatgaaac
    11941 tgaaagttat agaaaagcct gcaaaaaggg tgaccattac aataggttct ttggcctcgg
    12001 aagagtaatg tctagtaaga atagcaaaac agggatcata aaggcaggcc cccatagcaa
    12061 tgcccactac agcccacaaa atataaaatt cctccaggga actaaccata ggaataaaca
    12121 aaaggcaaac tcctcctaac actacccctc ccgacatcaa tgcccggcca agtcctttat
    12181 caattaattt cccagaccct atcccagtaa tcccagacac aatcaaggca atcatgagtg
    12241 ctccagacag ttcggatatg ctccaatcaa aatgtaattt ccaccgaatc agagaggcgg
    12301 gaaacaaata gaaaagacct gcccatgcga gagtttcacc tagtgaaaca gcccaaagtc
    12361 gaggattcga ccatacggtt gatttaactt cataatgccg cataagaaga cgcctgttta
    12421 gaaatacgtc aaatatatca ctggtgaaac tcctgtgttt ctgaagaatc aagaaatgct
    12481 ttgtagaaga gaagctagtg atactatgac caatatcgaa gtaaggagca atgcgatgaa
    12541 cgatctaatt tatggatcag caacgaaaat agcaagtgaa atcagacaaa aaaatgtttc
    12601 gtgcctagaa gtaattgaga tgcatttaaa gcgcatcgag gttgtgaatc ctgcactcaa
    12661 tgcagtggtt caattgtgtt ctgaaagagc acttgctgaa gcaaaagcgg cagatgtgaa
    12721 aatatcaaaa aaagaaaata tcggcccatt gcatggagtc cctatgactc taaaagattc
    12781 gctggacact gcagaagtcg tatcgaccgg cgggacaaaa ggacgacaga actttattcc
    12841 agataatgat tcaactgtca cgtccagatt aagaaatgct ggagccatac ttttagggaa
    12901 aacaaatact cctgagttca ctttggctgg ggaaacggac aatctggtct atggacggac
    12961 taacaacccg tataacttga ctagaattcc tggaggatca agcggtggtg ccgcagccat
    13021 catagcctcc ggtggttcac cgctggacat aggaagcgac acaggtggaa gtataagaat
    13081 gccttcacat ttttgcggaa taaccggtat caaacccaat agtggacgga taccacgaac
    13141 tgggcacatt gtgaaccacg ctatgggagc tgtcgattct ctaactcaaa atgggcctat
    13201 ggcccgatac gtagaggatt tgatactaac tctacctatc ctgtgtggcc gagattggat
    13261 agatccagca ataattgaaa tgcccttagg agacccctat aaagttaaga tcgaaaattt
    13321 aagggtagcc ttttacaccg ataacggcat ccacgaaccg tctcaagcaa tccaagacgc
    13381 tgtaaaaagt gcagctatgg acctaggcga tttaggtgct acggttgaag aagatcgtcc
    13441 caatgcattg ggaatgattc ctgaaatcaa taatgcattg aacggaggtg atggaagaga
    13501 atgggtaaaa agattaatgg aatctgccaa tactaaagaa atatctccat ttctgcagaa
    13561 gcgaataaac aatgccgaac ctatatccac agctgaatac acagccaacc ttgagaggtt
    13621 agatcaatat cgcagtcaca tgttgtcctt tatgaaacac tacgacgtta taatcagccc
    13681 cacggctcct ttcactgcca ccgatcacgg ggaaacattc agcaacaaaa acaaaaacgc
    13741 ctttgcttat acaagcgcct acaacatgac ggggtggcct ggtacggtcg tcagatacgg
    13801 aacctgcgaa gagaatttgc caatcggagt gcagatcgtc tcacgaccat ggagagagga
    13861 cgtttcactc gcaatagcac tggaacttga aaaaatttca gggggatgga agaatccgaa
    13921 catctaaact ctgttgggtt caccaaaaaa gaattactca caaggcaaaa aaacaaaaat
    13981 agcagaaggg ggaataatgc gagaatcacc tgttataaca aaaatagaag ctattcatta
    14041 ttctcacaca ttggaagaag tgagtcggga ttacaacact tttaacatgg tgtataaacc
    14101 aggggggaaa tccaaacaat cgggaacttt ggtgagaata cataccgatc aaggtctaac
    14161 cggagagtat cctgatgtta ccggcccaac tctagctgaa ataaaaatgt tgagttcata
    14221 ccttattggg aaaaatgccc taacacgtga actaatatat aacgacttga aaagaggctc
    14281 caggcattgg gcaatgctgg gaatgggaat tgtggatatt tgcctctggg acatagcagg
    14341 caagttttat ggagaaccct tattccgctt actgggaggc gacctgcaac cattaaaagc
    14401 ttatgcatcc accttgcatg gcgatgagaa tggtggtcta tcaacagctg gagatttcga
    14461 agatttcgca cataaatgcc ttcaaatggg ataccaggca ttcaaagtgc atggatgggg
    14521 tttagcacgg gacaatatta atcgtgagat tgaaaatgtt ttaaacctag ggaataaatt
    14581 cgaaggaaaa cttgatctgc ttatagaccc tgcctgtgaa ataaaaaatt ttggtgacgc
    14641 tttaaaactt ggaaaggctt gtgacgaggc caatttcttt tggtgggaag acccgttcca
    14701 agacaatggt gtttctcaat ttgcgcatag gaaattgaga caattggtga aaacgccctt
    14761 gcttcaaact gagcacattc gcctacttga acagcacgtt gatttcatcg tagctgatgc
    14821 taccgattat gtacgagccg gagcacacga agatggagga ataacggggg cgatgaaaat
    14881 agctcatgca tcggaaggat tcgggctcga cgtagagctc cacggtccag gcccagtcca
    14941 taggcatatc atgtcttcta taagaaacac caatttttat gaacttgggc tggtacatcc
    15001 aaatttacca acaacaaaag ctcctgtata tttggattat agtgatgatc ttgatgctat
    15061 agatcaagag ggaaatgttt atgcaccaca aggacctggt attggagtaa acctggattg
    15121 ggactggatt aacttccata aaaccgggga agaaattatc gctgaataat aaaatacaac
    15181 attccgactt aggatgggga aatcaaaatg agcaccacag aaggagcgtt agcaggagta
    15241 cgggtagtaa cttgttctac agcgcaggca gggacagtgc catatatgct gatggcagat
    15301 ctaggagccg aggtcataaa gattgaagtt ccaggaacag gtgacggttc aagacgtgca
    15361 ggcgaaatca aaaatggggt aagttccttt tttgaaacca acaatagggg agtgaaaagc
    15421 ctcactttga atctgaaagc agaagaaggg aaaaaaattc tacatgaact ggtaaagaga
    15481 gcagatgtat ttggacagaa ttttagaccc ggtgctgcag aaagaaacgg gtttggatat
    15541 gaagaattaa gaaagattaa tcctcgacta gtttacgcct ccgtgtcagc ttatggatta
    15601 gaaggtcccg acgcaaatct gccaggaaca gacgccgttg gacaagctct atccgggatc
    15661 actgaagcct tctctatccc agggcaatct atgcgaactg gagtagcctc agtcgcggat
    15721 gaaactacag caattcttac ttttggaggc gttttagctg ccttgattcg tgcaagggag
    15781 accgggaaag gacagaaggt agacacttcc ttagtaggta gtgcttttcg gctaatgggt
    15841 tggactatgg cgacaacaat gtggaaaaac caagctccga ttactggggc tcgaataaac
    15901 ggtagccgta acagggcagg aatcgctgcc tgttttaacg acatcaacgg taagcctcta
    15961 gcaattcaat tagacgcccg gcactggatc tcagctcttc aaattttggg atttcacaac
    16021 ttaatggagg aaaaaggttt gcttgatttg ggactggcct tcgattctga gaacaagaaa
    16081 aatgaaatcc ttgataccct ctcatcgctg tttgcaacaa atacccgcga acattgggta
    16141 accattttgc gagaggcgga tatggttgca gccccagtaa acacaatgct ggaagcatct
    16201 aacgatccta atgttgtcgc caacggctat gttaaagaaa tttttcaccc aaaagtcgga
    16261 gaaaatatca aaatccatgg gtcaccttgg aatttttctg aaactcctag taatccagga
    16321 tttgccccgg aattagggca acataatgat gagattctat cttctttggg ctacagtgaa
    16381 tcccaaataa aagagttgat tgatattgaa gttatataag ggattaattt ttgaggagca
    16441 cataataaaa catggctgat atacgaaaaa acagagccaa acaaaaatta aaaaatggag
    16501 aaactgtcat cgcagttgca tgtacggaca ccgatatggt cgattttcta gggtcatctg
    16561 gagctgtcga tgtcatctgg atagaaatgg aacacgggga agcgacttgg tcacaaataa
    16621 gtgatatatc tagagcgtgt gacctatggg gtatgacatc gcttgtaaga gtaaataaca
    16681 atgatccttg gcttgtgggc agggctttag ataggggagc acaagccgtg gttgtaccgc
    16741 acgtaaacac aaaagaagaa gcgaaaaggc tagttcaagg aggaaaatac
//
LOCUS       DASCNS010000064         1909 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000532831, whole genome shotgun sequence.
ACCESSION   DASCNS010000064 DASCNS010000000
VERSION     DASCNS010000064.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000532831"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            117..1349
                     /locus_tag="QF838_02565"
     CDS             117..1349
                     /locus_tag="QF838_02565"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HJO59608.1"
                     /translation="MSLVVQKYGGSSLATPELIKSVAARIRSAHDSEGNVVVTVSAMG
                     DSTDNLLSIASEISETPDLRELDVLLSTGELQSCALVAMALKSMGKKAVSLSGAQAGI
                     RTDSSYGKAKIAGVDTGRINEELEKGNIVIVAGFQGVTHDSDITTLGRGASDLTAVAL
                     AAGLKADRCEIYTDVEGIYTADPRLVSDARKMTQVGFEEMLELASYGAKMNPRSIELG
                     MVFQVPILVASSFNNEPGTLIHDGDNMDNTVGEIRNRVRGIATDKDVAKITVFSVVDR
                     PGIAAALFVPLAESDISVDVIVQNASVSGTTDLTFTIKQSDVDRAMKVVEGVAEDLGT
                     TNVEKAENLAKISIVGTGMQDSPGYASTMFKSLAEAKINIEMITTSEIRITCIIGSDQ
                     VAKAAEVLHAAFELEKPD"
     gene            1374..>1909
                     /locus_tag="QF838_02570"
     CDS             1374..>1909
                     /locus_tag="QF838_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJO59609.1"
                     /translation="MTDIHLSIVIPAYNESHRIKQTLDAIYEYLGNQNYSYEIILCDD
                     GSHDSTVRLSREFFGSKSNFQILSLPHKGKGSAVKTGMMTAQGQYRFMCDADLAMPLE
                     QIERFLKEMTDGCDIAIGSRELINSKRLGEPILRYFAGRVFNLFVKLIAIRKYQDTQC
                     GFKCFSSQAADKIFPLVRT"
BASE COUNT          568 a          327 c          469 g          545 t
ORIGIN      
        1 acacgatcgg ctatgagatg gctgatgctg ctatgaaatc tggtcttgac ggagagattg
       61 ttttcacgtc tcctacttca aaaggcgcat acatagagaa aaattaaagg attacattgt
      121 cacttgttgt acagaaatac ggtggatcat ccttagctac cccagagttg attaagagcg
      181 tagctgccag aatacgatcg gcacatgact cggaaggaaa tgtggtggtg actgtttctg
      241 ccatgggcga ctccacggat aatttattgt ctattgcttc tgagatttcg gaaacaccgg
      301 acttgaggga acttgatgtg ttgctatcaa caggtgaact gcaatcgtgt gcacttgtgg
      361 ctatggccct taaatctatg ggtaaaaagg ctgtcagttt gagcggtgct caggccggaa
      421 ttcggactga ctcttcttac gggaaagcta aaatcgctgg tgtagatacg ggtaggatta
      481 atgaggaatt agagaaaggc aatattgtaa ttgttgctgg cttccaagga gtgacccatg
      541 atagtgatat tacaactttg ggaagagggg cgtcagattt aaccgctgtc gctttagctg
      601 caggattaaa ggctgatcgt tgtgagattt atactgacgt agagggaata tataccgctg
      661 atcctcgttt agtttcagat gctaggaaaa tgactcaagt tggatttgaa gaaatgctgg
      721 aattggcgag ctatggtgca aaaatgaatc cccgttcaat tgaattgggc atggttttcc
      781 aagtgccaat actagttgct tcaagtttta ataatgaacc gggcactcta attcatgatg
      841 gagacaatat ggataatacg gttggagaaa tacgaaatag agtacggggt atcgctacgg
      901 ataaagatgt tgccaaaata acggtttttt cggtggttga tagaccggga attgcggcgg
      961 cattatttgt tcctttggct gaatcagata taagcgtaga tgtcatagta cagaacgcta
     1021 gtgtctcagg taccacggat ctgacgttca ctataaaaca gtctgatgtg gatagagcaa
     1081 tgaaagtggt tgagggagtc gccgaggatc ttggtacgac taatgttgag aaagctgaaa
     1141 atctagccaa aattagtata gttggtacag gtatgcaaga ttctcctggg tatgcttcga
     1201 ctatgtttaa gtctcttgct gaggctaaga tcaacatcga aatgattacc acctcggaga
     1261 ttaggattac ctgcataatc ggatcggatc aggtagcaaa agcagcagag gtactgcatg
     1321 cagcatttga gttggagaaa cctgattaat attaaaactt agtagttaat gaagtgactg
     1381 atattcacct ttcaatagtg attcccgcct ataacgaatc gcatagaata aagcaaactc
     1441 tcgacgccat ttatgaatat ctgggtaatc aaaactattc ctatgaaatt atattgtgtg
     1501 acgatggtag tcatgattcg accgtcaggc tatcgaggga attctttgga agtaaaagca
     1561 attttcaaat cctatctctt cctcataaag gaaagggatc agctgttaag acaggcatga
     1621 tgactgccca gggccagtac aggtttatgt gtgacgctga tcttgcaatg ccattggaac
     1681 aaattgaaag gttcctcaaa gaaatgacgg atggttgtga tatcgcaata ggatcaaggg
     1741 aattaatcaa ttctaaaaga ttgggagaac ctatacttcg ctattttgca ggcagagttt
     1801 ttaatttatt tgtgaaactt atcgccataa gaaaatatca agacactcaa tgtgggttta
     1861 agtgtttttc ttctcaggct gccgataaaa ttttcccact cgttagaac
//
LOCUS       DASCNS010000065         1957 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000537183, whole genome shotgun sequence.
ACCESSION   DASCNS010000065 DASCNS010000000
VERSION     DASCNS010000065.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000537183"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(60..1211)
                     /locus_tag="QF838_02575"
     CDS             complement(60..1211)
                     /locus_tag="QF838_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HJO59610.1"
                     /translation="MKAVVYKKPFEVAVETVDDPKIKHPNDVIVKITSSCICGSDLHM
                     YEGRTAAEPGIVFGHENMGIIEETGSAVKNLSVGDRVVMPFNVACGFCKNCQRGYTGF
                     CTTVNPGFAGGAYGYVAMGPWVGGQAEYMQVPFADFNCLPLPKGDQFEADFSLLADIF
                     PTGYHGAELADVSPGESVAVFGAGPVGLMAAYSSVIRGAAEVYCIDHVQERLDKAASI
                     GAIPINFDDGNPVEQIKDIRNDTGVDKGIDAVGYQAAAQGSSAAGGEQEEVPNVVLNQ
                     MIDVTKPTGMMGIPGLYVPSDPGGVNESAKSGALLLNFGKLFEKGLRLGTGQCNVKKY
                     NAYLRDLIIAEKASPSFVVSHEVSIDDAPDAYTKFDQRVDGYTKVILHP"
     gene            1400..>1957
                     /locus_tag="QF838_02580"
     CDS             1400..>1957
                     /locus_tag="QF838_02580"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO59611.1"
                     /translation="MKYLKTIGFNSNQPIGRGFNYPYDIAFSEDQRIFVLNRMGGHNS
                     SGIRIQICTYDEDWLGEFATGPGKGDSQFVIPVCMSFNSKDLLYVTDESLNDVKVFDN
                     QGNYITKWTTNITGPQKFAGPSGIVHGSDDSVYLVEQYANKISKFTEDGQFLFSWGSE
                     GEGEGEFNLPWGISKDVEGHLFIADW"
BASE COUNT          562 a          469 c          381 g          545 t
ORIGIN      
        1 catcacattt cctaaggtgt cgtagtgggc tgaaaattta ttttcagccc acagtctatt
       61 taagggtgaa gaattacttt agtgtaacca tccacccttt gatcaaattt cgtgtaggcg
      121 tctggggcat catcaataga aacctcatgc gaaaccacga aactggggct agccttctcg
      181 gcaataatca aatccctgag ataggcgtta tatttcttta cgttgcattg tccagtgccc
      241 aaacgcaaac ctttttcaaa cagtttgcca aaatttaaca gtaaagcgcc cgatttagct
      301 gattcattga ctcctcctgg atcagaaggc acgtacaatc ctggtattcc catcattcct
      361 gtgggtttgg ttacatcaat catttggttc aaaaccacat tgggaacctc ctcctgctct
      421 ccaccagcag cagaggagcc ttgagctgct gcttgatatc cgacagcgtc tattccctta
      481 tcaactcccg tgtcattcct tatgtcttta atctgttcaa caggattacc atcatcgaag
      541 ttgattggaa tcgcaccaat actcgctgct ttatccagcc gctcctgaac gtgatctata
      601 caatacactt cagctgcccc tctaatcact gagctatagg cagccatcaa tcccacaggg
      661 cccgcaccaa acacagccac actctcacct ggactaacat ctgcaagttc ggccccgtga
      721 tatcccgtag gaaatatatc cgccaacaag gagaaatcag cctcaaactg atctccttta
      781 gggagaggca aacagttaaa gtcagcgaaa ggtacttgca tatactcagc ctgtcctcca
      841 acccacggtc ccatggctac ataaccatat gcaccacctg caaatccagg gtttaccgtg
      901 gtgcaaaacc ctgtataacc tctctggcag tttttgcaaa acccacaggc aacattgaaa
      961 ggcatcacga ccctatcgcc aaccgatagg tttttaactg cagagcctgt ttcttcgata
     1021 attcccatat tttcgtggcc aaatacgata cctggctcag cggctgtccg gccttcatac
     1081 atatgcagat cagatccgca aatacaactc gatgtgattt ttacaataac atcgttaggg
     1141 tgctttattt tgggatcatc aaccgtttct acggcgacct caaatggttt tttataaaca
     1201 acggccttca ctgtgtttcg cctccttgtt ttatatggct aacctactca gaacagggca
     1261 catattatta ttaatacagc cctctgacaa atcgttatat gtaataaaac ataagaaaat
     1321 atgttttaat ctctttatta tagttttagg gtgttattat ccgttcaaat aaaaccactt
     1381 ttaagttggc ggtactgaaa tgaaatattt gaaaaccatc ggatttaatt caaatcaacc
     1441 cataggccgt ggcttcaatt atccatatga tatagctttt agcgaagacc agaggatatt
     1501 tgtgctcaac cggatgggag ggcataactc gtcaggtata agaattcaaa tctgcaccta
     1561 cgatgaagac tggttaggtg aatttgctac aggtcccgga aaaggtgata gccagtttgt
     1621 gataccagtt tgtatgtcat tcaacagcaa agatctactg tatgtgactg acgaatcact
     1681 caatgacgtc aaggtatttg ataaccaagg caactacatc accaagtgga ctacaaatat
     1741 tactggacct caaaaatttg cagggcccag tggaattgtg catgggtcag atgactctgt
     1801 ttacctggtt gaacaatacg caaacaaaat aagcaagttc accgaggacg gccaattcct
     1861 cttctcatgg ggttccgaag gtgaaggtga aggtgagttc aatttaccgt ggggaatttc
     1921 taaagatgta gaaggacacc tctttatcgc agattgg
//
LOCUS       DASCNS010000066        12585 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000539800, whole genome shotgun sequence.
ACCESSION   DASCNS010000066 DASCNS010000000
VERSION     DASCNS010000066.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 12585)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12585)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12585
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000539800"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="QF838_02585"
     CDS             <1..218
                     /locus_tag="QF838_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59612.1"
                     /translation="LPMLRFTGLGTESCTVSVARFVVLAWLNLHFQVNVGKLGKRDIN
                     VIRATGFLYALMGKAISHGNKIAIFDN"
     gene            305..1168
                     /locus_tag="QF838_02590"
     CDS             305..1168
                     /locus_tag="QF838_02590"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HJO59613.1"
                     /translation="MKSVLEAINKRRQKPFYLCDFSPPKGGNADLLLESYDLNPDMFL
                     VAYAPGKSVRLNPIFAADWIHSRTNIPSVFTVSTRDMNKSAMQSLLLGAELMGLPNVL
                     IVKGDKFSAEDLNLQTEVNDFTPTELISGVKTMNERRDFRGNELTYPTRFCIGGALDL
                     SRDWGKESWLTKAKITSGCNFIVSQPTFDPELPSKFLSFYEKTIGEQLKIPVMWGIQM
                     IEKDTISFANVPSWVYQELEEGGSSYEITINLVKRYLELGIDTFYLVPTIYKGGRRDY
                     LSAQAVMEDIE"
     gene            1293..1409
                     /locus_tag="QF838_02595"
     CDS             1293..1409
                     /locus_tag="QF838_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59614.1"
                     /translation="MANETGKRYSCGKCGSEFVVTRGADGTVSCCGEPMSQK"
     gene            1457..1600
                     /locus_tag="QF838_02600"
     CDS             1457..1600
                     /locus_tag="QF838_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HJO59615.1"
                     /translation="MSNQLGRRYQCDGCGTTVLCTKAGDGVIQCCDLGMELQQPRKLP
                     SSD"
     gene            complement(1640..2524)
                     /gene="lipA"
                     /locus_tag="QF838_02605"
     CDS             complement(1640..2524)
                     /gene="lipA"
                     /locus_tag="QF838_02605"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HJO59616.1"
                     /translation="MNTERRLPTWLKVKMPSGPNYSKLQTLIKTQGLHTVCEEARCPN
                     IGECWEKETATFMILGDICTRACTYCAVTTGRPTNLDLEEPKRLANTVKILGLKYAVI
                     TSVNRDDLPDGGAFIFAQCIRQIKKRVPTCKVEVLTPDFQGDWKALKIVTDANPETFN
                     HNIETVKRIFPRVRAKGDYQLSLRLLDRVKDFMPDGVTKSGMMVGLGETKTEIIETME
                     DLLQHRCDLLTIGQYLRPSQKHAPISKWYTPEEFADLEKIGMEMGFKHIASGPLVRSS
                     YHADEQHSAAYSKTLTVA"
     gene            2665..3906
                     /locus_tag="QF838_02610"
     CDS             2665..3906
                     /locus_tag="QF838_02610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HJO59617.1"
                     /translation="MVDSGRAVTYSGDPLRNLDPQKEFAELRLLSMGAFGDTVANFED
                     GFIQVFGGIVGEVVKARIYRFKRRGKNKINGMVSEVIKASPHRVDSPCPYFGPCSGCQ
                     WQHVSYEYQLELKRGAVRHVFADYASLSNVHVSKTNPAPEIFNYRNHARFTVRFGGQL
                     GFSNRITRRFVRVDECMLMDSKINSFLSQLQDNSSETTNLSIRVGANTDDYLIQPELS
                     GPDIDFETGQKWYVENMLNREFRVASPSFFQVNTRQAEFMINLVGDKLGLNGDEILVD
                     AYAGVGVFASLLSDRVRKVIAVEESQAAVKDALFGLNDLQNVHYIQGKTEDILEELEE
                     KIDVIILDPPRTGCHPQAIASVLSAEPNQIAYVSCDPPSLARDLDMLVQGGYQIDSVD
                     PIDMFPQTYHTECVATLSRKN"
     gene            3968..4663
                     /locus_tag="QF838_02615"
     CDS             3968..4663
                     /locus_tag="QF838_02615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HJO59618.1"
                     /translation="MDEKNKQKKITVFATSSPRRKQMLHQFGLDFRFVDPIGNERIKH
                     ENESPLEYVQSLATQKARSVFEVVPNNAIIFAADTVVVLGDQVIGKPINRTEAEETLK
                     NLSGSHHQVITAICVFDTAKDVYLEEAVATKVHMRDYCYGEIRSYVETGDPMDKAGSY
                     AIQNDFFNPVEAIEGCYFSVVGLPICTLLKILTQLKLKTIIDVEKHKRDFSKCTGCLM
                     LSPLNRSQIITEL"
     gene            4686..5042
                     /locus_tag="QF838_02620"
     CDS             4686..5042
                     /locus_tag="QF838_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59619.1"
                     /translation="MEILVILLISFLLLGPQKMISGARILGDLISEAKRFAEKIPHID
                     VDNSATTRNNNINEVESDGNKSQLDLGQDTATPVAFQSRKKKTPAPEPPGLLGEEEIN
                     DKKTGTEDESDKSSGE"
     gene            5049..5801
                     /gene="tatC"
                     /locus_tag="QF838_02625"
     CDS             5049..5801
                     /gene="tatC"
                     /locus_tag="QF838_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HJO59620.1"
                     /translation="MRDDSQSLGTHLDELRRRLTWSAIVVVIFTVVAFIFHQQILRLL
                     MEPASGFPGVPNQKPIYTDLTEFIGIAMKASLLAGIFTSLPFLLWQLVMFVSPGLNPT
                     EKKYLYILMPVSVIIFLLGAAFGYFVLFPPAVKFLITFGSDVATPMIRIGNYVGLMLS
                     LLFWMGVIFELPIVLFFLSRIGVVRPAFLSRNRKWAIVLSFVLGALITPTLDPINQTL
                     VAIPVLVLYEVGIILSKLGVKIRYRESPDDFE"
     gene            complement(5894..6067)
                     /gene="tatA"
                     /locus_tag="QF838_02630"
     CDS             complement(5894..6067)
                     /gene="tatA"
                     /locus_tag="QF838_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HJO59621.1"
                     /translation="MPFRFGPMELIIVLVVVLIIFGVGRLPEIGGAMGKALKEFRSTQ
                     KDITEEDSTTKKE"
     gene            6299..6643
                     /gene="rplS"
                     /locus_tag="QF838_02635"
     CDS             6299..6643
                     /gene="rplS"
                     /locus_tag="QF838_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HJO59622.1"
                     /translation="MDAHNLIPVEKNPNIEDFGPGDTVIVNVRVVEGERVRTQAFEGV
                     VLRVRGNGRGETFTVRRITNQVGVERTFLKCSPNVESVKVTRKGKVRRARLYYLRGLS
                     GRAARIKEDRRS"
     gene            6732..9203
                     /gene="priA"
                     /locus_tag="QF838_02640"
     CDS             6732..9203
                     /gene="priA"
                     /locus_tag="QF838_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HJO59623.1"
                     /translation="MKFVEVAVDSPGSGNRTFTYSIPSGLSLELGHSVVVPFGNQTKQ
                     GIVFGVSSTSDLDSVREALELTDRDPLIDPIRLELARWVSQQYFCTLFQACSLMFPPG
                     ARKRKSVWLSMASGVEISELKFGDFQTSVLKYLSSRKAAQLEKVVAHFGERARGSVTR
                     LVEKQVVVRTVKESSVKVRVKYEKLGKITKSGKLALKAGKLLKAPKQLAALTFLTNEE
                     DTVHRLATLRGKFGSSSINSLISRGYLDQEQLEVERDPLNGKSYTPAQPVNLSDSQTY
                     ALEQISELIENPSIKTRAVLLHGVTGSGKTEVYLDAVSLCISRGKNSLVLVPEIALTP
                     QTIERFSSRFPGKIAVLHSGLTVGERFDQWRKIRANEYQVVIGSRSSIFAPLNNLGLI
                     IIDEEHEWTYKQNDGVPRYHARDVAMQLAGLTSAAVILGSASPDIESYRRAKRGRYRL
                     VELHHRYRPDAVNGSSNRDDRLLAEVNIVDMRAELKSGNNDIFSRQLVAELERTISNG
                     NQAILFLNRRGFSSLVQCNSCGYVAKCRNCDVSLTFHAESKLLICHYCAQRRRDIEKC
                     LECGSDSVGKYGVGTQLVTEKLNKLYPHVVIDRWDSDAIAKNSHYGELLDRFRSGRSQ
                     IVVGTQLISKGHHLPNVTLVGVVSADVGLGLPDFRASERVFQTLCQVAGRAGRGTEDG
                     KVIIQTYQPDHYAIKTAATQNYQSFFDIESMNRRNHVQPPYSKIIRLLRQDVDDISGQ
                     REAKQLAKLLEEQRQIWGLSDTEILGPTQAFPSRVRGRYRWQLILRGPNPRTLLETIS
                     LPGVSDERRSMKRGWVLDVDSYLSI"
     gene            9321..10422
                     /gene="prfB"
                     /locus_tag="QF838_02645"
     CDS             join(9321..9386,9388..10422)
                     /gene="prfB"
                     /locus_tag="QF838_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139044423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HJO59624.1"
                     /translation="MQELKTALFDLREKVSNLLVRLDLPSKERKIKELEKESIAPNFW
                     EDSADAQSKMKTLAALNNQVVLWKGFEEQIMTNLELLELANSENDPSFLDEIRLETGR
                     IEKRVGDEEFTLTLSGEHDQRDAILALHAGAGGVDSQDWASMLVRMYTRWCDRKGFGC
                     EVLDLSHGEESGIKSVVMQITGHYAYGHLKAEKGVHRLVRISPFDNDHARHTSFALAE
                     VIPKMDEGTDVEINTDELRVDVFRAGGHGGQNVQKNSTAIRITHEPTGIVVTCQNERS
                     QRQNREIAMRILASRLAELERERKDKEMSDLKGDHVSAEWGNQIRSYVLHPYQMVKDH
                     RTNVETSATDEVLDGNLDVFIKAYLAFQMGSE"
     gene            10556..12277
                     /locus_tag="QF838_02650"
     CDS             10556..12277
                     /locus_tag="QF838_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HJO59625.1"
                     /translation="MINRFALVFSMLILLIGSIACGQNAIGKAVGDSGNSDSAIGTSN
                     VAESIQESTKEKRADLQTEVKTLADVSEAPVGVFNRLWSDPPTLDPHLTSDTTSAGVV
                     VEIFGGLVGFDTSLRLIPDLADSWDISNDGLTYTFKIRNNAVFHDGTKVTAEDFKWSL
                     ERAVNPDTASPTAKTYLGDIVGVAAVLEGKSSEISGVKAVDDSTLQIVIDSPKAYFLA
                     KLTYPTAYVVDRNNVSVGGLNWTDSPNGTGPFKLSDYKIGERIILERNDLYHLDKAKL
                     AKIQMNLAGGSSMAMYENDEIDITGVGLFDLERIKDPVDPLNGDLVVADPGYSVNYIG
                     FNVQEPPFDDSKFRRALNHAIDKELIAKEVYSDLVVPAYGILPPGFPGFNEQLEGLRH
                     DPVLAKKLLSESKYADPGTRPRIVITIPGTGGTPGLDLEVIINMWLETLGVEVEIQQV
                     EWATYLKDLDEEKFQAFGGLGWEADYPDPQDFLDILFHSESENNHGLYSSQKADILLQ
                     DARIETDLVKRIDLYQEAEKIIVNDAAWVPLWFAGERHVLIKPYVKGYELTPMTISKM
                     RYVYIED"
     gene            12395..>12585
                     /locus_tag="QF838_02655"
     CDS             12395..>12585
                     /locus_tag="QF838_02655"
                     /inference="COORDINATES: protein motif:HMM:NF040248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59626.1"
                     /translation="MPALLLMVSAVTFVLGTYGPGDPVRVMLGTKYGEETADRLRSSL
                     GLDRPVIVQYADYISGAIRG"
BASE COUNT         3667 a         2315 c         2923 g         3680 t
ORIGIN      
        1 tgttacccat gttaaggttt accgggttag gaacagagtc atgtacggtg tcagttgccc
       61 gttttgtggt tctggcatgg ctcaatcttc actttcaggt aaacgtaggg aagttaggga
      121 agagagatat aaatgtaatt cgggccacag ggtttcttta tgccctaatg ggaaaggcga
      181 tatcgcatgg aaataagata gccattttcg ataattaaaa aagccctcca tttcaacggg
      241 gggctttttt tattggtata gttgcgaaag aattcttcaa taatttaatg ttccaccctt
      301 ggttatgaaa tctgttttag aagctattaa taaaagaagg cagaaacctt tttatctatg
      361 tgatttttct cctcccaagg gaggaaatgc cgatttgtta ctcgaatcct acgaccttaa
      421 tcctgatatg tttttggtgg cctatgctcc tggtaaatct gttagactaa atcctatatt
      481 tgcggcagat tggattcact ccagaacgaa tatcccatct gtttttaccg tctccacccg
      541 tgatatgaat aaaagtgcga tgcagagtct tttattagga gcagaattga tggggctccc
      601 taacgtttta atagtcaaag gggataaatt ttctgctgag gatctgaatt tacaaactga
      661 agtgaacgac ttcaccccca cggaattgat atccggtgta aaaacaatga atgagcgccg
      721 tgattttcga ggaaatgaat taacttaccc aacacgattc tgtatcggcg gggctttgga
      781 tctttcgaga gattggggaa aggaatcctg gttgactaag gcaaaaatta ctagcggctg
      841 caacttcatt gtttcacaac cgactttcga tcctgaactg ccttctaaat tcctttcatt
      901 ttatgaaaag acaattgggg agcaattgaa aattccggtt atgtggggta ttcaaatgat
      961 agaaaaagat actatttcat ttgcgaatgt tcctagctgg gtgtaccaag aactagaaga
     1021 gggagggtca tcatatgaaa tcacaatcaa tctggttaaa agatacttag aacttgggat
     1081 tgataccttt tatcttgttc caacaatcta taaaggtggg agaagagatt atctgtcagc
     1141 ccaagcggtg atggaagata ttgaataatc tgttccttta cgtgtgatgg tatttaattt
     1201 aggaatgttc ttaggcgaaa cgtcgttgac cttcaacctg tcccatttta taattcgggc
     1261 tcgttatcga tactatgaaa gaggtgaaca ttttggctaa cgagacaggt aagcgttatt
     1321 cttgtggtaa atgcggatcg gaatttgttg tcacaagagg tgcagatggc acggtgtctt
     1381 gttgtggcga accgatgtct caaaaataaa atagtgaatt gtctgattat aaatatgggt
     1441 ttgaggggtt ttacgaatgt ctaatcaact tgggaggcga taccaatgcg acgggtgcgg
     1501 aaccactgtt ttatgtacta aagcagggga tggggtcatt caatgttgcg atctaggtat
     1561 ggagcttcaa cagcccagaa aattgccttc atcagactga ttaattttat gccctagcag
     1621 gcggttctta attcaccagc tatgcaaccg ttaatgtttt tgagtaggca gcactgtgtt
     1681 gttcgtccgc atgatatgaa cttctcacaa gtggtccaga cgctatatgc ttaaatccca
     1741 tttccatacc gattttttcc aaatctgcaa attcttccgg cgtataccat ttcgagatgg
     1801 gagcatgctt ttgggaaggt cttaggtatt gcccgattgt tagtaagtcg catctgtgtt
     1861 ggagcaggtc ttccatagtc tctataatct ctgtctttgt ttctccaagg cctaccatca
     1921 ttcctgattt ggtaactccg tctggcatga agtccttaac tctgtccaac aatctcaaag
     1981 aaagctggta gtcgcctttg gcccgtactc tggggaatat tctttttacg gtttctatat
     2041 tgtgattgaa ggtttccgga tttgcgtcgg tcacaatttt gagtgctttc caatcaccct
     2101 ggaagtcagg tgttaaaacc tcaaccttac atgtgggaac tctttttttg atttgccgaa
     2161 tgcattgagc aaatatgaag gctcccccat ctggtaaatc atctcggttc actgaggtaa
     2221 taaccgcgta cttcaagccg agaattttta cagtgttggc taatctcttt ggttcctcaa
     2281 gatcgagatt ggttggccgt cccgtggtta cggcacaata tgtgcatgct ctggtacaaa
     2341 tgtcccccaa tatcatgaag gtagctgttt ctttttccca gcattcccct atattgggac
     2401 acctggcttc ttcgcaaact gtgtggagac cttgggtttt aattaaagtt tgcaatttag
     2461 aatagtttgg tccactaggc atcttgactt taagccatgt gggtaaccgt ctttcggtat
     2521 tcatcgatgg gagactccac tattttatta accggcctac gtaccatcat gatagcagag
     2581 gtaatagaaa atcacgtaat acactttaca gtttcggcgt ttcgggagac tatcaaatca
     2641 caaatataaa tggttgaggg ttgaatggtt gattcaggca gagcagtaac ctatagcgga
     2701 gatcctttga gaaatctgga cccacaaaag gaatttgcag aattgcgact tctttcaatg
     2761 ggagcttttg gggacaccgt ggccaatttt gaagacggat tcattcaagt ttttggaggc
     2821 atcgttgggg aagttgtgaa agctcgcatt tatagattca agagacgtgg taaaaacaag
     2881 ataaatggta tggtctctga ggttataaaa gcctcgccgc atcgagtcga tagtccctgc
     2941 ccctattttg gtccatgcag tggatgccag tggcagcatg tgtcctatga atatcagcta
     3001 gaactaaaaa gaggtgcggt caggcatgtt tttgcagact atgcctctct ttcaaatgtg
     3061 catgtgtcca aaaccaatcc agctccggaa atttttaatt atcgcaatca tgccagattt
     3121 actgttcggt ttgggggtca gcttggattt tctaaccgga taaccagaag attcgtcaga
     3181 gttgatgaat gtatgttgat ggattcgaaa atcaatagct ttctatccca actacaagac
     3241 aattcctcag agacaaccaa tctatctata agagtgggag cgaatactga tgattatcta
     3301 attcaacctg agttatctgg gccagatata gattttgaaa cgggacagaa gtggtatgta
     3361 gaaaatatgc taaacagaga gtttagagtt gcgtctccat cctttttcca agtaaacacc
     3421 aggcaagcgg aatttatgat aaatcttgtc ggtgataaat tagggttaaa cggggatgaa
     3481 attcttgtcg atgcatacgc aggggttggt gtttttgcct cattactttc ggacagagta
     3541 agaaaagtga ttgcagtgga agagtcccaa gctgctgtaa aagacgcact atttggatta
     3601 aacgacttgc agaatgttca ctatattcag ggaaaaactg aagacatatt ggaagaatta
     3661 gaagagaaaa tagatgtaat tatcctcgac ccaccaagaa ccggatgtca tccccaagcg
     3721 atagcttcgg ttctgtccgc ggagccaaat caaattgctt atgtatcctg tgatccacct
     3781 tctctagcca gagatttgga tatgctggta cagggaggat atcaaatcga cagtgtagat
     3841 cctatagata tgtttccgca gacataccat actgaatgcg tagctacttt gtctcggaaa
     3901 aactaactca taataaattt ttgtaatctg acttccgata ggtcgatacc ctatggatag
     3961 ttttcacatg gatgagaaaa ataaacagaa aaaaataact gttttcgcaa cttcgtcacc
     4021 gagaagaaaa cagatgttgc accaattcgg gttggatttc agatttgtag accctattgg
     4081 aaatgaaaga attaaacatg agaatgaatc tcctcttgaa tatgtccagt cattagcaac
     4141 acaaaaggct agaagcgtat tcgaggtggt tcccaataat gcgataattt ttgcagccga
     4201 tacagtcgtg gtgttagggg atcaggtgat tggtaagcca ataaatagaa ctgaggcaga
     4261 agaaacctta aaaaatttat ctggcagtca tcatcaggtg ataaccgcaa tctgcgtgtt
     4321 cgatacggcc aaagacgtgt atcttgaaga agccgtcgca acaaaagttc acatgaggga
     4381 ttattgctac ggtgaaataa ggtcatacgt agaaacaggg gaccccatgg ataaggccgg
     4441 gtcttatgca attcaaaatg acttttttaa cccggttgag gccatagaag gatgttattt
     4501 ttcagttgtg ggcctcccta tatgcacatt attaaaaatt ttgactcaat tgaaattaaa
     4561 aacaataatt gatgtggaaa aacacaagag agatttctcg aaatgtacgg gatgtttgat
     4621 gttgtcaccc ttaaatagat ctcaaataat tacggagtta taacctcatg ctgggaatgg
     4681 gaagcatgga aattttggtt atattgttaa tatcttttct tttgcttggg ccacaaaaaa
     4741 tgatttctgg agcacggata ctgggagatt taatctcgga ggctaagaga ttcgcagaaa
     4801 aaattcccca tattgatgta gataattctg ccactactag aaataacaat atcaacgagg
     4861 tggaatctga cggtaataag tcacaacttg atttgggcca agatactgca actcctgtgg
     4921 cttttcaatc gcggaagaaa aaaacccctg cacctgaacc gcccggactt ttgggtgaag
     4981 aagagataaa tgacaaaaaa acaggcactg aggatgaatc tgataagagc tcaggggagt
     5041 gatgtccatt gagggatgat tcacagtctc tagggactca ccttgatgaa ctgcgaaggc
     5101 gtctaacgtg gtctgcaatt gttgttgtga tcttcacggt tgtcgctttc atttttcatc
     5161 agcaaatact tcgtttgttg atggagcctg ctagtgggtt tcctggtgtt ccaaaccaaa
     5221 agcctatata cactgatttg accgaattta ttggcatagc tatgaaagca tcactgcttg
     5281 ccggaatttt tacctcattg ccttttcttt tatggcagct agtaatgttc gtttcacccg
     5341 ggctaaatcc cactgaaaaa aaatatttat acatattgat gcctgttagc gtcatcattt
     5401 ttttacttgg tgctgcgttt gggtactttg tattgtttcc accggcggtc aaatttctta
     5461 ttactttcgg tagtgatgtt gctacaccaa tgattaggat tggtaactac gtgggcctga
     5521 tgctttctct tctattctgg atgggagtga tattcgaatt acccatcgta ttgtttttcc
     5581 tatctaggat cggggtagta cgaccagcgt ttttatcaag gaaccggaaa tgggcaattg
     5641 tgctctcatt cgtgttagga gctctaatca cacctacatt ggaccctata aatcagacct
     5701 tggtagctat tccagtttta gttttgtatg aggtagggat tattttgtcc aaattagggg
     5761 tgaaaataag atatagagaa tctccagatg attttgaata aaactggaaa caagggtaat
     5821 tcgtgattcg agtacatttg agtttacatc ctctgaatgt attaaacttt tgcaccatgg
     5881 agtaaacccc aggctattct tttttagtcg ttgaatcctc ttcggtgata tctttttggg
     5941 tggacctgaa ttccttgagg gcttttccca tagcgcctcc aatttccggt aacctgccta
     6001 caccaaatat tattagtaca accaccaaaa cgattattag ttccatgggg ccaaatctga
     6061 aaggcattat ctcctcccca ctttgtgaat gaattgcgtc agtatcgaca gcttcaattc
     6121 tatcaaatac tgtgaatctg ttcccaaatt atttttttag gatgaataca agccaaatgc
     6181 gtatcgaacc tattgccttt gtagcaaata tcacagtaca attttagcct tgtaattcgc
     6241 ctctgaacta ttttattctc aggttaacga acttaaaaaa tatgttggga cacgtaaaat
     6301 ggatgctcat aatctgatac cagtagaaaa aaatccaaac attgaagact ttggccctgg
     6361 agatactgtt attgttaacg tgcgtgtggt tgaaggggaa agggttagga ctcaggcctt
     6421 tgaaggagtc gttctgagag tcagaggaaa tggaagagga gagacattca cagttcgtag
     6481 gattaccaat caagtaggtg tggaacgaac ttttcttaag tgttctccaa acgtagaatc
     6541 tgtcaaagtt acaaggaagg gaaaagtcag aagggctaga ctgtattatt tgagaggtct
     6601 gtcaggtagg gccgccagaa ttaaagaaga cagaaggtct tgatttccta aaccgaagtc
     6661 tcagtttctc cccttctata attgtatact tcaggacaaa tgaccttagt taggtaacga
     6721 ggttgagtct tttgaaattt gtcgaagtag cagtagattc gccgggttct ggcaatagga
     6781 cctttactta ctcgatcccg tcagggcttt ccttggaact aggtcactct gtggttgtac
     6841 cctttggtaa tcagaccaaa caaggaatag tttttggggt ttcaagtaca tccgacttgg
     6901 attcggtacg ggaagcattg gaactcacag acagggaccc tttaattgac ccgattcgct
     6961 tggagttagc ccgttgggtc agccaacagt atttttgtac gctatttcag gcctgttccc
     7021 taatgtttcc cccaggagcc aggaaacgta aatctgtctg gttgtctatg gccagtgggg
     7081 ttgagatatc agaactgaaa tttggagact tccagacttc cgttctgaaa tatttgtctt
     7141 ctaggaaagc tgcacaactg gagaaagtgg tcgcccactt cggagaaaga gctcgagggt
     7201 cggttacccg gcttgttgaa aagcaggtag ttgtccggac tgtgaaagaa tcctcagtca
     7261 aagttcgggt gaagtatgaa aagttaggta aaataaccaa atcaggtaaa ttagctttaa
     7321 aggcggggaa attacttaaa gctccaaaac aattggccgc cctcactttt ctaactaatg
     7381 aagaagatac agtacatcga ctggctaccc taaggggcaa attcgggtct tcttccatta
     7441 attcactaat atccaggggt tatttggacc aggaacaact cgaagttgaa agggaccctc
     7501 tcaacggtaa atcgtataca cccgctcagc cggttaatct ttcagattca caaacttacg
     7561 ctttagaaca aatatctgag ttgatagaga atccttctat taagaccaga gctgtccttc
     7621 tccatggagt taccggtagt ggtaagactg aagtgtattt ggatgctgtg tccttgtgca
     7681 tttcccgggg gaaaaattcc ttggtgcttg ttccagaaat cgcactgacc ccccaaacta
     7741 tagaaaggtt ttcttccagg tttccaggaa aaatagcggt tctgcatagc ggattgacag
     7801 taggggaacg atttgatcag tggaggaaga tcagagctaa tgaatatcaa gttgtgattg
     7861 ggtcaagaag ttccatattt gcccccttaa ataacttagg gttaatcata attgacgagg
     7921 aacatgaatg gacttataaa cagaatgatg gggtgcctag gtatcatgca cgagatgttg
     7981 cgatgcaatt agcaggatta acatcagccg ctgttatttt aggttcagcc tcccctgata
     8041 ttgaatccta ccggcgagcc aagcggggaa gatatcgcct tgtagagtta caccatagat
     8101 atagaccgga tgctgtcaat ggcagttcaa atagagacga cagattgctt gccgaggtca
     8161 atattgtgga tatgagggct gagttaaagt ctggtaataa tgacattttc agcaggcaat
     8221 tagtagccga attagaaaga actatcagta atggaaatca ggccattctt tttctaaaca
     8281 gaagaggttt ctcttctttg gtccaatgca atagctgtgg gtatgtagcc aaatgtagaa
     8341 attgtgacgt gtcactcaca tttcatgctg agtccaaact attaatatgc cactactgtg
     8401 ctcaaaggag aagggatatt gaaaaatgtc tggagtgtgg tagcgattct gttggtaagt
     8461 atggtgtcgg gacacaactt gtgactgaaa aacttaataa attgtaccct cacgtggtaa
     8521 tagataggtg ggatagtgat gctatagcta agaattccca ctacggggaa cttttggaca
     8581 ggttcagatc aggacgatct caaattgttg ttggtactca gttaatatca aaaggacatc
     8641 atctgcctaa cgttacatta gtaggtgttg tatcagctga tgtcggtctt gggctgccag
     8701 atttcagagc atctgagcga gtatttcaaa ctttgtgcca ggtagctggt agagcaggga
     8761 gaggaacgga ggacggtaaa gttattattc aaacctacca accagaccat tacgctataa
     8821 aaactgctgc tacgcagaat taccaatctt tttttgatat agaatcgatg aataggcgta
     8881 accacgttca gccgccttat agcaaaataa ttagattact tagacaagat gtggatgaca
     8941 tttctggaca gagagaggcc aagcagctag cgaagctatt ggaagaacaa agacagatat
     9001 gggggttatc ggataccgaa attcttggcc cgactcaagc ctttccaagt cgagttagag
     9061 gtagatatag atggcaacta atattaagag gcccaaaccc ccgaactttg ctggaaacaa
     9121 taagtttgcc tggtgtttct gatgaacgta ggtctatgaa aagaggatgg gtattagatg
     9181 tggattctta cctgtcgatc tgatcgtttt ggatggcatt gctggtgttt aaattaaata
     9241 gccataccga tccccttggt ataattgttt ttagcgttca gatgtgcttt ttataagaac
     9301 tgtttggaga ggaacccctg atgcaagagt tgaaaactgc tttattcgac ctaagagaaa
     9361 aggtttctaa cctattggtg cgtctttgac cttccttcaa aggaacgaaa aataaaagaa
     9421 ttggaaaaag aatctatagc tccaaatttt tgggaagatt ctgctgatgc ccagtccaaa
     9481 atgaaaacct tggcagcatt aaataatcag gttgttttat ggaaaggatt cgaagaacag
     9541 ataatgacga atctggaatt acttgaactg gctaattcag agaatgatcc aagctttctg
     9601 gatgagattc gcttggaaac gggccgaata gaaaaaaggg ttggagatga ggaatttacc
     9661 cttaccttat ctggtgaaca cgatcaaaga gatgccatac tagccttaca tgcaggggca
     9721 ggaggtgttg attctcaaga ttgggcttct atgttggtcc gtatgtatac cagatggtgt
     9781 gatcggaaag ggtttggctg tgaagttcta gatctttctc acggggaaga atctggtata
     9841 aaaagtgtgg tgatgcaaat aactggccat tatgcatatg gccatctcaa agcggaaaaa
     9901 ggagttcata ggctcgttcg gatttcacca ttcgataacg accatgctag gcatacctca
     9961 tttgctcttg cagaagttat accaaagatg gatgaaggta ccgacgttga aattaataca
    10021 gatgagttga gggtggatgt attcagagcc ggtggacatg gcggacagaa tgtccagaaa
    10081 aattcaacag ctatacgaat aactcatgaa cctactggca tagttgtcac atgtcaaaat
    10141 gaaagatccc aacgccaaaa cagagaaata gctatgagaa tattggcctc tcgtttggca
    10201 gagttagaaa gagagagaaa agataaagaa atgtcggatt tgaaaggcga tcatgtttct
    10261 gccgagtggg gtaatcaaat tcgtagctat gtattgcatc cgtatcagat ggttaaggat
    10321 catcgcacca atgtggagac atcggctaca gatgaagttt tagatggcaa cttggacgtc
    10381 tttataaaag catatttggc cttccaaatg ggttctgagt gacgctttaa ttactcaaga
    10441 tttccgctaa ggtgataata cctaagtgaa attgccgttg ttaatattca agaaagatca
    10501 ctattattaa agagacctgt tttttgttta agattaaatt gattttgagg tatatatgat
    10561 taaccgtttt gcactcgttt tttcaatgct catactttta ataggatcaa tagcgtgtgg
    10621 tcagaatgcc ataggaaagg ctgttggtga ttcaggaaac tcagactcag ccattggaac
    10681 ctctaacgtg gctgagtcga tacaagaatc cacaaaagaa aaaagggcgg atctgcaaac
    10741 tgaagtaaaa accttagctg atgtttccga agcaccagtt ggcgtattta accggctttg
    10801 gtctgaccct ccaacactag atcctcatct cacttcagat accacttctg caggtgttgt
    10861 tgtggaaata tttggcgggt tggtaggatt tgataccagc ttgcgattga ttcctgatct
    10921 cgctgatagt tgggatattt caaatgacgg ccttacttat acctttaaaa tccggaataa
    10981 tgctgtattt catgatggga ctaaagtcac agcagaggat tttaagtggt ctttggaacg
    11041 agccgtaaac ccggatactg cttcgcctac ggccaaaaca tatttaggcg acatagtggg
    11101 agtagccgct gtgctggaag gtaaaagttc tgagataagt ggtgtcaaag ctgtcgacga
    11161 tagcacttta caaatagtga ttgattctcc gaaggcatat tttttagcca aacttactta
    11221 tcctacggca tacgttgttg acagaaataa tgtatccgta ggaggtctta actggaccga
    11281 tagccccaat gggactgggc catttaaatt atcagattat aaaattggag aaaggataat
    11341 attggagcga aacgacctgt atcacctcga taaagcgaag ctagcaaaga tccagatgaa
    11401 tttggccgga ggttcctcca tggccatgta tgaaaatgat gagattgata ttacgggagt
    11461 ggggcttttt gatctggaac gaatcaaaga tccagttgac ccacttaatg gagatctggt
    11521 cgttgctgac ccaggatatt cagttaacta cataggtttt aacgtacaag aacctccttt
    11581 tgatgattct aaatttcgtc gcgcattaaa ccatgcgata gacaaggaat taattgcaaa
    11641 ggaagtgtat tccgatttgg tggttcctgc gtacgggata ttgccaccag gctttcctgg
    11701 gttcaatgag cagctcgagg gtcttcggca tgatcccgtt ctagccaaaa aacttttgtc
    11761 cgaatcaaaa tatgctgatc cggggactag gccaagaatc gtaataacca tcccaggcac
    11821 ggggggtact cctggattag acctggaggt aataataaac atgtggctgg aaaccttggg
    11881 tgttgaggtg gagatacagc aagtcgaatg ggctacatat ctcaaagatt tggacgagga
    11941 aaaattccag gcttttggcg gtttaggttg ggaggccgac tatcctgacc ctcaagattt
    12001 cttggacata ttatttcatt cggaaagtga aaataaccac ggcttatatt ctagtcagaa
    12061 ggctgatatt ttactccagg atgctaggat tgagacagac ttggtgaagc gaatagatct
    12121 ttaccaagaa gcagagaaaa tcattgttaa tgatgctgct tgggttcctt tatggtttgc
    12181 aggagaacgg cacgttttaa taaagccata tgtgaaaggg tatgagttga ccccaatgac
    12241 gatatcaaag atgcgatatg tttatataga agattaaact tgcgattaat cgtacctaaa
    12301 ttattcacag tcctctgatg tcgtttatgc tataacgaat tcagagtaag tttagaggca
    12361 tatgtggata tatataacta gaagattact atggttgccg gcattattgt taatggtgtc
    12421 ggcagttacc ttcgttctcg gaacatatgg gcctggtgat cctgtgaggg tgatgctagg
    12481 aactaaatac ggcgaggaga ctgcagacag acttagatct tcattggggc tggataggcc
    12541 tgttatagtt caatacgctg actacatatc tggggcaatc agggg
//
LOCUS       DASCNS010000067        15270 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000544815, whole genome shotgun sequence.
ACCESSION   DASCNS010000067 DASCNS010000000
VERSION     DASCNS010000067.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 15270)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15270)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15270
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000544815"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="QF838_02660"
     CDS             <1..181
                     /locus_tag="QF838_02660"
                     /inference="COORDINATES: protein motif:HMM:NF018717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HJO59627.1"
                     /translation="GGEILADDTTWQPKRIVILEFPTREDMDKFSNSEEYKPVAALRH
                     KAADTEGFVVLGYDG"
     gene            complement(178..1056)
                     /locus_tag="QF838_02665"
     CDS             complement(178..1056)
                     /locus_tag="QF838_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59628.1"
                     /translation="MIESEEQEPIIPNQEPTFVAVIVPVATAENKQSTLQAPSPIPIS
                     TSVPIPTGTPVTIREIFKSEVEDKTLQKKTFEAVSKINSFKYILEGIARVDAGGIQVG
                     VPLSASGAVTSSVSLSKFEANLLGIGLYVESAKTGDDIYTRESSLEEWKDSRKLGIGH
                     ISDDFLKAVGDSFLKSHLSGSDILTTQEKTIFLFNDDGPVGSVTPLFELIATEEEARR
                     FSPEKWKVNLEVDSDNYEISKIISEFSLSNGGQFLSEVFGMEGLSGVGNTDVELVIKF
                     SQIGEDFSIVIPQPAH"
     gene            complement(1146..2126)
                     /locus_tag="QF838_02670"
     CDS             complement(1146..2126)
                     /locus_tag="QF838_02670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HJO59629.1"
                     /translation="MPEKKVFVTRIIVPDAIAKLKQNFEVEIWDQPTPPPKELVIQKA
                     KECDGMMIESNDIMDDEVFIAAGRLKVVGTRAIGIDNIDLESATKNGVLVGNTPGILH
                     ESCADFTMGLILSLARQITRSNRKVIAGEWKIFDQTPYLGTDVYGKTLGLVGLGLIAT
                     AVAKRAKGFDMEILYHSRTRKPDVEDMYGLKWIPDLDRLLSASDYVSVHVPLGPETQG
                     FIGSKEIGNMNENAFLINTSRGGTVDPDALREALIAGKIAGAALDVTFPEPISPDDPL
                     LHMENVIITPHIASASAATLRRMGLMAADNVIAHLRGETMPACLNPEAVG"
     gene            2291..3496
                     /locus_tag="QF838_02675"
     CDS             2291..3496
                     /locus_tag="QF838_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59630.1"
                     /translation="MKESSQQNIPYRWVMLAGVWLAYFSFGVVQGGIPPLIGPVSQDL
                     GLSRTTMGTVLGAWPLIYILTAIPAGALLDRFNLKFTIMVGVIFIGISGLLRAIAVDY
                     KTILFAVMVFGLGGPFISIGAPKLISTWFSESDRGRAMGIYLTAPAVGRILVLAATNS
                     LLMPLYNNSWRLTLLTLAGVAFITAIIWIFLARDLRPNSLKPSDKKDLLASMKTFPDL
                     VKVREVRLILIIVLGMFLFNHTTNNWLPEILVEKNLSSSKAGLLAAIPVATGAFGTAI
                     IPQFAKPNAHKRIVFTLFWIAAACTICLIYFENSLLLSVLIVLGIASRGVMPIIMLTL
                     ISMVGGEKTGAAGGLYFTAGEVGGVLGPILLGFSADIFNDFTPGLFFLSFLCIALSFG
                     STIVTKKLI"
     gene            complement(3503..3721)
                     /locus_tag="QF838_02680"
     CDS             complement(3503..3721)
                     /locus_tag="QF838_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59631.1"
                     /translation="MKPAVDRLEAQLDEKLIFVRLEVQSHVGKILYSKYEGKLVPTFI
                     IFDQFGAIRYTTNGVPDYTSVNSMFVDN"
     gene            complement(4058..4249)
                     /locus_tag="QF838_02685"
     CDS             complement(4058..4249)
                     /locus_tag="QF838_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010645776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59632.1"
                     /translation="MAKYSCGDCGMEVHPITCGKCGTELTHVNIEKDDGTTVGVAECT
                     NGCGKIKSPMCCGHDMAAA"
     gene            4470..5393
                     /locus_tag="QF838_02690"
     CDS             4470..5393
                     /locus_tag="QF838_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013595.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO59633.1"
                     /translation="MATKPLRVSYGEYTYENVPEWAPLPSGWEWNHVVGVCVDSQDRI
                     FAYNRSEHPMIVLDKNGTILGTWGEDSFSSAHHVQVGPADSIWTTDIGDHTVRKWSAE
                     GDQLMMLGTPNEPAPEQSGEPFNKPTDIAFGLDGSIYISDGYGNSRIHHYTPQGKLIG
                     SWGERGTGPGQFMIPHGVEVDPSGLVYVADRENSRIQIFTSSGQYVSEWRGVHRPNEI
                     LHGSDGAMYVAELGMRQGTSPDADEVTVVTPHSGVKIMTKNGQWLGGWGADTHTAGDL
                     VAAHAVGVDSEGSLYVGETLDGARLQKFIRV"
     gene            complement(5383..6684)
                     /locus_tag="QF838_02695"
     CDS             complement(5383..6684)
                     /locus_tag="QF838_02695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59634.1"
                     /translation="MPSEITQNSSENSPRGIYKGWVVVAVSFLSIGGSIGFAQYGFGL
                     FIVPLQEEFGWTRTQINFAITLGVLGHFVSPLIGRTIDVFGSRYVMAVSLGFIAVGFF
                     LRSMMAELWQFYLFSLLVFTGTPGTGLATGRLVQLWFPETRGRMMGFVTAGNNFGGMI
                     SIPVLTLVIAFGGWRSGFLFTAIFLGVLSIASFIFVRDGIEFVLKEQNKRWAPRGLNQ
                     YSSKKVFDGLSVRSALLTRAFWFIATGMTLQQFVRTTLVTQLAAHLQDIQLSPDKIGL
                     CLSVLAFGGVASKIIFGRVSENTTARWAYAIVVGIQVVGVAAFVFFSSDNFVLIFISL
                     SIFGLGMGGIGALGPLAVTELFGMKNYGTLNGLIRQGVIIPGIVGPLLAGAIYDSQGS
                     YDLAFKIILGFLFLSFLCFVSASPPARKEDFRDQRAVDSKL"
     gene            complement(6745..9828)
                     /locus_tag="QF838_02700"
     CDS             complement(6745..9828)
                     /locus_tag="QF838_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HJO59635.1"
                     /translation="MSYITDLALRRGSVTVLAFIIILGAGIFTFISLPVEVLPRVQFP
                     LLTVNVDYLNAGSEEVVEAVTQPLEQHISGLGNLESIQSTSVEGKSILLLFYEYGSNM
                     DQAKKDLEARLVSLELPDGATVPNVRGIDPGSEPILQLSLTRSEGDFSSLSDVAESQI
                     EPSLNSVQGIQNVAVTGNQIFDLLVALDHDALARFDISMADISKILAESNFSVPSGYL
                     FDGSKMVSVRTSNSVSSIEDVKQISVGSTDGKVITLIDVAIVSLVPSPTNSISRTNGI
                     QSLGISVTKDPDANTVDVSAVALSKVMAIANAEKIQVVEIYNGGPEIESQLNTLKSEG
                     MYGFIFAVIGVFLFLLNVRGGVLKGLTLSLRPTSVIALTIPISILGGVLLMGFTDLTL
                     NIMTLGGLAISVGRVVDDSIVVLENVYRHMQRGESPKVAALNATREVAAPITASTLTT
                     IVVFAPLGFIQGIVGSFFSPFCLSVTFALISSLLVSVTFVPVAGAFLLRLGDLTPGDD
                     TEPSLTTIQRIYKPTLEWALTHKLASVGIALILCVGSLGLLRFIPVTLFPAGPDRYIS
                     IDISVSPSATTEEKDAAVLSVESELERLRIAGNVENYLTTIGGIGIASFPGAGPLESA
                     NIFVNIDNSAPSDIADTLRDALPSTDGRTYRVEEGTGGPPAAGLELQIIGTSFEDVTR
                     ANSQLMEAITTVKGIENIESDLTGLRPEMLLKVDSEAASKIGLTPREVAQRVSEPMKG
                     QTVGNYVIDGKDVKVILRVGRGMYGGMGSFDDVMISGPLGDVSIEELVDPVLQEAPIA
                     VGRTNGKRSATITGMITDEDARAVGLEVEKIRYSVEQNNPGVIIESGGIFADIAEGFQ
                     DIFLAMAISIVLVYLVMVGSLGSLRNPFVILFSLPLAGIGALVGLAVTGSSLGLPSMM
                     GLLLLIGLVVTNAIVLIAFVQQLQDKGMSVRDSLIEGGVVRLRPILMTAITTAAALIP
                     LAVFVGDEGGGIIGSDLAIVVIGGLASSTFLTLLIIPIIYEFMHETIPNLLRLKR"
     gene            10047..11318
                     /locus_tag="QF838_02705"
     CDS             10047..11318
                     /locus_tag="QF838_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59636.1"
                     /translation="MNKSATNSNKYGSTWNPLNPINGWWAVAVCFSISTALVGFSTYT
                     FALFGDEMSAEFGWTRTQINASLSFLAIGQILGPLFGRIIDSKGPRIIVALCVSLTAL
                     SYIMRPMMTELWHWYALSFLQYAGFSATMTPVGRIVGLWFPNTRGRVMGIAMMGNNFG
                     GVVMPGIIGAVIALGGWQSGYYLQGLLTSLVAVAVLLALREPKMGLLTLKVPSNLSST
                     SSEPAQLPGMSISEAARSKTLYILLYIFLVGATPFASLLPQLISHYVNEGVNSTTAAT
                     AMSILALAGMSGKLVFGILGEKITARYAMMVSFFGLSVALLLSVNPTVPGIIWVCSPL
                     WGLCMGAFGTLTNMIVQDYFGLKSFASIMGVAGYGTAVSFAVGPLLAGLSYDNLDSYR
                     PIFYGTVIVMAIGILALNAMGTPERNFMSKN"
     gene            complement(11376..11867)
                     /locus_tag="QF838_02710"
     CDS             complement(11376..11867)
                     /locus_tag="QF838_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HJO59637.1"
                     /translation="MDTEILRLANLTADLLIETNQTIGIAESSSGGLISAHLLAIPGA
                     SKFFMGGSVIYTRVAQRNLLGVNDEEMEGLRASTEQYASLNAKMIRKLLGTTWGLSET
                     GATGPTGNRYGDSAGHSCIGVSGPLSRTKTMETGEEKREENMLSFTKASLVFLIGCLE
                     ENR"
     gene            11978..12127
                     /locus_tag="QF838_02715"
     CDS             11978..12127
                     /locus_tag="QF838_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59638.1"
                     /translation="MSKGWFILVGLFSLSLVLVTGGLVKGNVELTAAGFVIGIGIFFI
                     RNRLK"
     gene            12708..13502
                     /locus_tag="QF838_02720"
     CDS             12708..13502
                     /locus_tag="QF838_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59639.1"
                     /translation="MATFNRKGIHNWESGKRGFQSVLNDCQQIGLLLIWSQNYWKNSV
                     GRQFLESRYRITLPTRTLTAYKTRLKTLWSKGSGALDEPAEWSDFKALLTNGVPERHL
                     YNLHGRWRDIQKICLDVGVTPIRPTYRALKWWAYVSEYYGNNMSAASDRQIIGDQLMV
                     RELISERTQTPFINEDIEMWLIHEPWRGQTNMRKYLNLVAEKKITPIDWGKDGWGLEI
                     ADSLERTDQYPKDYGLSMLNWLLGTSPEPYLLPSQIIERYSIFARP"
     gene            13555..13917
                     /locus_tag="QF838_02725"
     CDS             13555..13917
                     /locus_tag="QF838_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SaoD/DsrE family protein"
                     /protein_id="HJO59640.1"
                     /translation="MKVAYILNTPNAANYKVGTMILPQLEEDRHGVDVLGIFFFDDNT
                     YMLRKGDPKGERLATIAKEKGMLLMMCDQCAIQRELATGEAGGAEPKGTVDGVSVGCF
                     PDLYAALAPNPPDQIISL"
     gene            complement(13924..14835)
                     /locus_tag="QF838_02730"
     CDS             complement(13924..14835)
                     /locus_tag="QF838_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59641.1"
                     /translation="MALHEEDLKKLGLHERPPVVRELRSIWIFIRSWPVIPIVILGLL
                     ILSGLLADVLATQDPRQGGIRDRHIPPAWTEEGTTEHILGTDHAGRDVFTRTLYGARI
                     SLAVAAAALLSGFIIGTTLGIVSGYAGGWIDELITRIVDIWQALPFLMVALVCVMIFG
                     QSTTLLLSLMVLLAWHPFVRVIRSQTLTIKNQPYVDLARVAGASLPRILLRHILPGVI
                     NTAVVIATLSVGSLILAEAALSFLGAGVPAPTPAWGIMVAEARGYLKTAWWPAIFPGM
                     SIFLVVLSLNFMGDWLRDKLDPRLRQL"
     gene            complement(14846..>15270)
                     /locus_tag="QF838_02735"
     CDS             complement(14846..>15270)
                     /locus_tag="QF838_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59642.1"
                     /translation="GNLKFYILPWMVLGWPAAAGIMRLTRSSMLEILDSEYIKLARAK
                     GVARLPLIWKHGLKNAIIPPLTSMLVLLADYLNGALVVEIVFSWPGIGQVALNQAVYD
                     NDWPLLVGTVFSFIGIYVSFAFIADLLYAFIDPRIRYS"
BASE COUNT         4591 a         3419 c         3023 g         4237 t
ORIGIN      
        1 gggaggtgaa attttggcag atgatactac ttggcaaccc aaaagaatag tcatattaga
       61 attccccacg cgggaagata tggataaatt tagtaattca gaagaataca aaccagtagc
      121 agctttgagg cataaagcgg ccgacactga aggattcgtc gtattaggat atgacggcta
      181 atgagctggt tgaggaatca cgatggagaa atcctcacca atttggctaa actttatcac
      241 gagttcaaca tcggtattgc ctacccctga taacccctcc ataccaaata cttctgaaag
      301 gaattgcccg ccgttactta gagaaaattc ggagataatt ttggaaattt cgtagttgtc
      361 agaatctacc tccaaattca ccttccattt ttcaggggag aatcttcgag cctcctcttc
      421 agtggcaatt aattcaaata atggagtgac agaaccaaca ggtccatcat cattaaataa
      481 aaaaatggtt ttttcttgtg ttgtcaaaat atcgcttcca ctaagatgag attttaaaaa
      541 agagtcccca acggccttca aaaaatcatc tgatatatgc ccgataccca actttcgact
      601 atctttccat tcctccaaag aagattccct tgtatagata tcatcacccg tttttgcaga
      661 ttccacatat aatcctatcc ctaacaaatt ggcttcaaat ttggataagg agacggaaga
      721 agttacggct cccgatgctg aaagtggcac acccacctga atacctcccg catcaactcg
      781 agcaatacct tcaagaatat atttaaatga atttatttta gagacagcct caaatgtttt
      841 tttctgaagc gttttgtcct caacttcaga tttaaatatt tccctaatgg ttacaggagt
      901 ccctgtcggt atcggaacgg aggtggaaat tggtatcgga gatggtgcct gaagagtaga
      961 ttgtttattt tcagcagtag cgacaggaac aattaccgcc acaaatgttg gttcctggtt
     1021 gggtattata ggctcctgtt cctcagattc gatcaaaaaa catcccggaa tggtaagtat
     1081 taaaaaaata cccatccatg tatggcttaa aaatcggata ctcatcatca ccaaaaagcc
     1141 acgggctacc cgactgcctc cggatttaaa caggcaggca tcgtctcacc tctaagatgt
     1201 gcaataacat tatcggccgc catcaaaccc attctcctaa gtgtggccgc gctggcgcta
     1261 gcgatatgag gagtgataat cacgttctcc atatggagca aaggatcatc cggcgatatt
     1321 ggttcaggaa aagtaacatc caaagccgct cctgctattt tcccagcgat taaagcttct
     1381 cgtagggcat ctgggtctac cgttcctcct ctggaagtat taattaaaaa agcgttctca
     1441 ttcatattcc caatttcttt tgaaccaata aatccttgag tttccggtcc gaggggaaca
     1501 tgaacagaaa cataatcgga agcagacaaa agtctatcca aatcaggaat ccacttaagt
     1561 ccatacatat cttcaacatc cggttttcgt gtcctggaat gatagaggat ctccatatca
     1621 aatccttttg cccgtttggc gaccgccgta gctatcaacc ccaaaccgac taaacccaaa
     1681 gttttgccgt acacatcggt acccaaatat ggagtctgat caaaaatttt ccattccccc
     1741 gcaataactt tcctgttgga gcgggttatt tgacgggcta aagaaagtat caatcccatt
     1801 gtaaaatcgg cgcacgattc atgtagtata ccgggggtat tacctactag aacaccattt
     1861 ttagtcgctg attcaagatc tatattgtca attcctattg ccctggtccc cacaactttc
     1921 aaacggccag ccgcgataaa cacttcgtca tccatgatgt cattagactc gatcatcatt
     1981 ccatcacatt ctttggcttt ctgtataacc aattcctttg gaggaggtgt tggttgatcc
     2041 cagatctcta cttcaaaatt ttgtttcaac ttggcaattg catctggaac tatgattcta
     2101 gtaacaaata ctttcttttc tggcatatat aaatctccta ttcatcacat gctcgggcga
     2161 aaaagtatac acctatatat caaatactaa actggcactg aagtttatat cttgcactag
     2221 tggggttagt acctcaaact cttattattg cgtgagccgt ttcgcatcgc acaggaaaaa
     2281 acgctcgtta atgaaagaat cttcacaaca aaatattcca tatagatggg taatgctcgc
     2341 aggtgtttgg cttgcctatt tctcatttgg ggtagtgcag ggcggtatac ctcctctaat
     2401 cgggccggtt agtcaggatc taggcttaag ccgcacgacg atggggactg tattgggagc
     2461 atggccatta atttatatat tgaccgcaat tccagccggg gctttattag atagattcaa
     2521 tctgaaattc acaataatgg ttggcgttat ctttattggt atttccggac tccttagggc
     2581 tatcgctgtc gattacaaaa caatactttt tgcggtaatg gtgtttggtt tagggggtcc
     2641 tttcatatcg attggtgcac caaaattaat tagcacatgg ttttctgaat ccgaccgggg
     2701 acgtgcaatg ggaatatatc taactgcccc cgcagtagga cggattcttg tgttagcggc
     2761 taccaacagt cttttaatgc ctctctataa caatagttgg agattaactt tgttgacgct
     2821 ggctggtgtg gcttttatta ccgcaataat ctggatattc cttgcaagag accttagacc
     2881 taacagtttg aaacccagtg ataaaaagga tctcttggca agcatgaaga ctttccctga
     2941 tctagttaag gtgagggaag tgcgcttaat tctaattatc gttcttggaa tgtttttatt
     3001 caatcacacg actaataatt ggcttcctga aattttagtg gaaaaaaatt taagttcatc
     3061 aaaagccgga ttgttagcag caataccggt tgctacaggg gcattcggta cagcaatcat
     3121 cccacagttc gcaaaaccca acgcacataa aagaattgtt tttacccttt tttggatcgc
     3181 cgcagcctgc actatctgtt taatctattt tgaaaactcc ctacttttga gtgtactcat
     3241 cgttctggga attgcatcaa gaggcgttat gccgatcatc atgctcacat taatatcaat
     3301 ggtagggggt gaaaaaacag gtgcggctgg agggctctat ttcacagcag gagaagtagg
     3361 tggagtttta ggtccaattt tattgggctt ttctgctgat atattcaacg attttacccc
     3421 tggattattt tttctttcct ttttgtgtat tgccctttca tttgggtcaa caatagttac
     3481 caaaaaacta atctagccat aatcaattat caacaaacat agaattaact gaagtgtagt
     3541 cagggacacc atttgttgtg taacgaatag ccccaaattg gtcaaaaatt atgaaagttg
     3601 gtaccaactt tccttcgtat ttgctataca aaatcttccc aacatggctt tgcacttcaa
     3661 gcctgacaaa gattaacttc tcatccagtt gtgcttccaa cctgtcgacg gcaggtttca
     3721 tagatagaca tccaattcaa aaatcggaat ataggtacag taatatggga cgatcagaat
     3781 tgtgcaatga gtcccaacca gaaatatcct ttatctcatc agatgagctg gtcaaggaat
     3841 tctggaaaaa aatcacacac accacaagga ttagaacgga caataaagcg atttttatcg
     3901 aaaaagtacg gtaggccaac aaaaaagcta ccgtcaaaac aaaaagagaa atcaatacgt
     3961 atgaatattg atttaagata gtgaacataa gtgatatcaa ataaaaatgg gtcgcttttg
     4021 agcgacccat ttcattactc tgttcaaatt agtccaccta cgcggcagcc atatcatgtc
     4081 cgcaacacat tggtgacttg attttgccac agccgttagt acattctgct acaccaactg
     4141 tagtaccgtc atccttctca atatttacat gggtcaattc tgttccacat ttcccacacg
     4201 taataggatg tacttccatt ccgcaatccc cacaactata tttcgccatt ttttcccctt
     4261 aattcacgct cattaataaa acagtatcaa gtgactcacg ttctctgcaa agagtctatc
     4321 gttcactaaa attactgtca aacaacacaa atggagattc catatattga atacattcaa
     4381 ttaacaaagt atagttccga tcctacaaaa aaacctctat ctaagtaagg cttctgcgat
     4441 cagacaacga atatttcaag gagaaagtaa tggcaactaa acctttacga gtaagttatg
     4501 gcgagtacac atatgaaaat gttcccgaat gggctccttt accaagtggc tgggaatgga
     4561 atcacgtggt gggggtatgt gttgatagtc aagatcgtat tttcgcctac aacagaagcg
     4621 aacacccaat gattgtatta gataaaaacg gaactatttt aggcacgtgg ggtgaggata
     4681 gtttctcatc ggcccatcac gtacaagtcg gccccgccga ttccatatgg acaaccgata
     4741 ttggtgacca tactgtacga aagtggtctg ccgaaggcga tcagttgatg atgctgggta
     4801 ccccaaatga gcccgcgcct gaacaaagtg gcgaaccatt taacaaaccc actgacatag
     4861 cttttggtct tgatggatca atctatattt ctgacggata cggtaactcc agaattcatc
     4921 attacacacc tcaagggaag ttgattggat catggggtga aagaggaacg ggacccggac
     4981 aatttatgat tcctcacggc gttgaagtag acccttctgg tctcgtttat gtggcagacc
     5041 gtgagaacag ccgaattcag attttcacat catcaggaca atatgtctcg gaatggagag
     5101 gcgtccacag accgaatgaa attttgcatg gtagtgacgg agcaatgtac gtggctgaac
     5161 ttggcatgag gcagggaact agtcctgatg cagatgaagt caccgttgtt actcctcatt
     5221 ccggagtcaa aattatgaca aaaaacggac agtggctggg tggatggggc gcagacacac
     5281 atacagctgg tgatttagtg gccgctcacg cagttggggt cgactcagag ggaagcctat
     5341 atgttggcga aactttagac ggtgcccgac ttcaaaaatt tattagagtt tagaatcgac
     5401 agccctctgg tctcggaaat cttctttcct tgccggaggg ctagctgaaa caaaacataa
     5461 gaacgataaa aacaaaaatc ccaaaattat tttgaaagct agatcgtaac ttccctgact
     5521 gtcataaata gccccggcta ataagggacc aactatcccg ggaattataa ctccctgccg
     5581 aatcaaacca ttcagagtcc cgtaattttt catgccaaaa agttcagtta cagccaacgg
     5641 cccgagagca cctatacccc ccattccaag accaaaaatc gataatgaaa taaaaatcaa
     5701 aacaaagtta tcagaggaaa aaaatacaaa ggccgccaca cctacaacct gaatccccac
     5761 aactatcgca taggcccacc tggcagtagt attctcacta acccttccaa atatgatttt
     5821 agaggctact ccaccaaatg ccagaacgga aagacataga cctattttgt caggtgaaag
     5881 ttgaatatct tgtaagtggg cagccagttg tgtgactaga gtggttctca caaactgctg
     5941 tagggtcatc ccggtcgcaa taaaccagaa tgccctggtt agcaaggctg accgaactga
     6001 taacccatca aacacttttt tcgaagaata ctgattaagg cctctggggg cccaacgttt
     6061 attttgttcc tttaacacga attcaatacc gtctcttacg aaaatgaagg aggcaatcga
     6121 caatactccc agaaaaattg cggtaaaaag aaatccgctt ctccaacctc caaaagcaat
     6181 aacaagagtt agtaccggta tcgaaatcat acccccaaaa ttgtttcctg cagtaacaaa
     6241 acccatcatg cgtccacgag tttcaggaaa ccataactgc accaaacgtc cagtggccaa
     6301 tccagtaccg ggggtaccgg taaaaaccag gagagagaac aaataaaatt gccataattc
     6361 agccatcatt gatctgagga aaaaccctac cgctataaag cccaaagaaa ccgccataac
     6421 atacctagat ccaaagacat ctatagtgcg acctattaaa ggtgaaacaa aatgacctaa
     6481 taccccgagt gttatagcaa aattgatctg agttcttgtc catccaaact cttcttgcag
     6541 gggaactata aagagaccaa acccatattg agcaaaacct attgaccctc caatagatag
     6601 aaaggataca gctactacta cccagccctt gtaaatacca cggggggagt tttcagatga
     6661 attctgggtt atttcagaag gcaacagaca ctatcaatcg aaaataaatc atccctgaag
     6721 actaacattt aattgtttaa gcagctatct ttttaatctt aaaagattag gaatcgtttc
     6781 atgcataaac tcataaatta tggggataat gagcaaggtc agaaatgtgg aactagccaa
     6841 gcctccgata acaacaatcg ccaaatcaga ccctataatt cctcctcctt catctccgac
     6901 aaaaaccgca agtggaatca aggccgctgc agtagtgata gcagtcatca atatcgggcg
     6961 caacctcact actccccctt caataaggga atcacgaaca gacataccct tgtcttgtaa
     7021 ttgctgtaca aacgctatca atacgatagc attcgtcacg acaaggccga tcaacaacaa
     7081 caaccccatc attgagggta accccaagga acttccggta acagctagtc caaccaaagc
     7141 acctattcct gccaaaggca agctgaaaag tattacaaac gggttcctca acgaccctaa
     7201 gctaccaacc atcaccaaat acactagaac aatacttata gccattgcta aaaatatatc
     7261 ttgaaacccc tcagctatat ctgcaaatat tccccccgac tcaatgataa ctccagggtt
     7321 attttgctca acagaatacc ttattttctc gacttccaag ccaaccgccc tggcatcctc
     7381 atcggttatc atacctgtta ttgtcgccga tcttttcccg ttggttcttc caaccgcaat
     7441 tggagcctcc tgtaacacag gatcaaccaa ttcctctata gaaacatctc caagaggacc
     7501 tgaaatcatc acatcatcaa aagaacccat acctccatac attcctctac caactctaag
     7561 tattactttt acgtctttac catctatgac atagttacca acagtttgac ctttcatagg
     7621 ttcacttact ctttgtgcta cttctctggg tgtaagtccg attttagaag ctgcttctga
     7681 atcaaccttt aagagcattt ctggtctcaa accagtaagg tcactttcaa tgttctcaat
     7741 tcccttaaca gtcgttatag cttccattaa ttgggaattg gcacgtgtta catcttcaaa
     7801 agaagtgccg attatctgta attcaagccc ggcggccggc ggccctcccg taccttcttc
     7861 aactctgtag gttcggccat cagtcgatgg gagagcatct ctcaaagtat cggcaatatc
     7921 agatggcgcc gaattatcaa tattcacaaa tatatttgca ctttctagtg gccccgcgcc
     7981 aggaaaagat gctatcccta taccgcctat cgtagtcaaa tagttctcga catttcctgc
     8041 tatacgcaat ctctcaagtt ctgattcaac gctcagaacg gcagcatctt tttcttcagt
     8101 agtcgcactg ggtgaaacag aaatgtcgat agaaatatat ctatctggtc ctgccggaaa
     8161 caatgtaacc ggaataaatc gcaaaagacc caagctgcct acacacaata tcaaggcaat
     8221 tccaaccgag gctagcttgt gtgtaagtgc ccactccaaa gtaggtttat atatcctctg
     8281 aattgtcgta agggaaggtt cggtgtcatc acctggggta agatctccca gtctcagtaa
     8341 aaaagcacct gctacgggga cgaaagttac tgaaacaagc agagaggaaa tcagagcaaa
     8401 tgtaaccgac aaacaaaaag gggaaaagaa agaacctact atgccttgaa taaaacccag
     8461 aggagcaaag actacaatcg tagtaagagt cgaagctgtt atcggagcgg caacttccct
     8521 tgttgcattt aaagccgcaa ctttagggct ttctcctctc tgcatgtgtc gatacacatt
     8581 ttcaagtaca actatactat cgtcgactac ccgaccgacg gatatggcca ggccccctaa
     8641 agtcataatg ttcaaagtga ggtcggtaaa acccatcaac agaacgcctc ccaaaatact
     8701 gatgggtatg gtcaatgcta ttactgaagt aggtcttaag gaaagtgtaa ggccttttag
     8761 aacaccacca cgaacgttaa gaagaaaaag aaacactccg ataacggcga aaataaaccc
     8821 gtacatccct tcacttttga gagtgttcaa ttggctttcg atttcaggtc ccccattgta
     8881 gatttccaca acttgaattt tttctgcatt cgcgatagcc ataactttag ataaggcaac
     8941 agccgaaaca tccaccgtgt tagcgtcagg atcttttgtg acacttatac ctaaactctg
     9001 tattccattg gtgcgagata tggaattggt cggggaaggc acaagagata caattgcaac
     9061 gtcaattagg gttatcactt tcccatcagt agaaccaaca gatatttgct ttacatcctc
     9121 gatcgaagaa acactattgc tagttcgaac tgaaaccatt tttgacccat caaataaata
     9181 tccggatgga acagaaaaat tactctctgc aagaattttg gagatatccg ccattgaaat
     9241 atcaaaacgt gccaatgcat catggtccaa cgctactaag aggtcgaata tttgatttcc
     9301 ggtaaccgct acattttgaa ttccctgaac agaattcaaa cttggttcaa tttggctttc
     9361 agccacatca gatagagaag agaaatcacc ttcactccgg gttaagctca actgcaatat
     9421 aggctcgcta cccggatcga ttcctctcac attgggcaca gtggctccat ctggcaattc
     9481 cagtgagacc aatcgtgctt ccaaatcttt tttcgcctga tccatattgg acccatattc
     9541 gtaaaaaagc agaagtatcg atttaccctc cacggaagtg gattgaatag attccaaatt
     9601 acctaaaccg gaaatgtgct gctctaatgg ctgagtcact gcttcaacca cttcctctga
     9661 accagcattc agataatcta cattaactgt taataaggga aactggactc tgggtaatac
     9721 ctcgacaggt aaggaaatga aagtgaatat acccgcacct aaaataataa tgaaggctaa
     9781 aaccgtcacc gaccctctac gaagggccaa atccgtaatg tagctcatat tagtcctttg
     9841 tagaatttac taatttcgtt ccattagcga tgatatgagc aaacagatag aaatcagata
     9901 acattgggta ttttttgtta cataaaacaa tttatattta taaatagtat gttaatgcac
     9961 attttcagtc aaccaattca atttgaggaa tcagactagc ttttgaatat agaaattctg
    10021 ctgacatttc acaataccga gacattttga ataaatcggc aactaactcc aacaaatacg
    10081 gttctacttg gaacccgtta aacccaatta atggatggtg ggcagtggcg gtttgtttca
    10141 gcatatcaac cgctcttgtc gggtttagca cctacacatt cgctttattc ggtgatgaaa
    10201 tgtcagcaga atttggctgg actcgcacgc agatcaacgc ttctctttcc tttctagcaa
    10261 ttggtcaaat cctcggtcct ttatttggtc ggattataga cagtaaaggg ccaaggatca
    10321 tagtcgcttt atgcgtaagt cttacggctt taagttacat catgagacca atgatgaccg
    10381 agctttggca ttggtacgct ttaagttttc tacaatatgc cggcttctct gccacaatga
    10441 ctcctgtcgg ccgaattgtt ggattatggt ttcccaacac acgaggcagg gtcatgggaa
    10501 ttgcaatgat gggtaataat ttcggtgggg tagttatgcc tggaattatt ggagcggtaa
    10561 ttgctctcgg gggatggcaa tccggttatt acctgcaagg attattaact tctttggtag
    10621 cggtggcggt acttctagcg ctacgggaac ccaaaatggg attgctaacc ctaaaagtac
    10681 caagcaacct atcgagcact tcttcagaac cggcacaact cccagggatg tccatatccg
    10741 aggcagcacg gtcaaaaacc ctctatattc ttctatatat atttctcgtg ggagctacac
    10801 catttgcctc tttattaccg cagctcatat ctcattacgt taatgaaggg gtgaattcca
    10861 ctacagctgc cacagctatg agcattttgg cacttgctgg gatgagtgga aaactagtat
    10921 ttggcattct gggcgaaaaa ataaccgccc gctatgccat gatggtatct ttttttggat
    10981 tatcggtagc cctcctattg tcggtaaatc ccactgtccc cggaatcatc tgggtttgct
    11041 ccccactctg gggtctttgc atgggagcat ttggaacact tacaaacatg attgttcagg
    11101 attattttgg cctgaaaagt tttgccagta tcatgggggt tgcgggatac ggtacggctg
    11161 tatcattcgc cgttggccct ttgctagctg gtttatccta cgacaacctt gatagctatc
    11221 gcccgatttt ctatggaact gtgattgtta tggccatagg aatccttgcc ttgaatgcta
    11281 tggggactcc cgaaagaaac tttatgtcca aaaattaaaa taaagacttc gggttaaata
    11341 tcaaattttt gcttctatct cataacgcaa caaacctaac gattctcttc caagcatcct
    11401 attaaaaaca ccaaagaagc cttggtaaaa gagagcatat tttcctctct tttttcctct
    11461 ccggtttcca tagttttagt gcgtgataag ggacccgaaa caccaataca agaatgtcca
    11521 gcggaatctc catatcgatt cccggtagga cccgtagcgc cagtttcact cagtccccat
    11581 gtagtaccca acaatttccg tatcattttg gcatttagag aggcatattg ctctgtggaa
    11641 gctcttaatc cttccatttc ttcatcattc actcccaata aattgcgttg ggctactctg
    11701 gtataaatca ccgatccacc cataaaaaac ttagaagctc cgggtatcgc cagtaaatgc
    11761 gctgaaatga gaccaccaga agatgattcc gcaattccga tagtttgatt agtttcaatt
    11821 agaagatccg ccgttaggtt cgccagtcgc aaaatctcag tatccatgcc tactccttga
    11881 tatacctact gaagactgcc ctaaacaatc tatttgtaat gtcctcttac cggttaagat
    11941 aaacgttaaa gtcgattaat acaaaggaaa gtattctatg agtaaggggt ggtttatcct
    12001 tgtcggtcta ttttctctgt cattggtgct cgttaccgga gggcttgtaa aaggaaatgt
    12061 tgaacttaca gcagccggtt ttgtcattgg gatcggtatt ttttttatcc gaaaccggct
    12121 caaataaatc tatctccaca cccatagttt tcggtaaagt tggctccttg accggagtgg
    12181 gctctactta atcgttttat taataaggca cccaacctct tatttaaaga accctaattg
    12241 ggatttattt cttctatcct ggcaatccgg cggcctaaat ttaccattcc ccaatatttt
    12301 tttcggaacg ttattcaagc aattaccata atgacttctt tactcatttc gttttactcg
    12361 ggtgcattta aagggctggg gccatattaa atgaaagctc aatcccgttt atcctgataa
    12421 ctaatacttg gcacctctgc aataggttgc cgtgaactaa atacaccata attttcgccc
    12481 cctcacccga aattatgaga ccacagttcc atagggtttt aaatacttac ttcgcaattt
    12541 tttttacatt ccccgattaa ttacatgttt aggaaatagc ctaacaaatc tttgaaatga
    12601 gtttttaaat atccttgaaa gatacaggaa tacgtgtata ttatttaagc agtataaata
    12661 ccatttcttt aatctatatt ttaaaatagg caacataaaa ctttaaattg gcaacattta
    12721 atcgtaaagg aattcataac tgggaatcag gtaaaagagg atttcaatcc gttttaaacg
    12781 attgtcaaca aattgggctt ttacttattt ggtcgcagaa ttattggaag aattctgttg
    12841 gtcggcagtt ccttgaaagc cgttatcgga ttacattacc aacaagaaca ttaacggcat
    12901 acaagacacg tcttaaaact ctttggagta aaggatctgg ggccttagat gagcctgcgg
    12961 agtggtccga tttcaaagct ctactcacta atggtgtacc tgaacggcat ttatataact
    13021 tacatggtag gtggagagat attcaaaaaa tttgtttaga tgttggcgtt actccaatta
    13081 ggcccacata tcgagctttg aaatggtggg cctatgtctc ggagtactac ggaaacaata
    13141 tgtcagctgc ttcggatcgt cagatcatag gggatcaact tatggtgaga gaattgatat
    13201 cagaaagaac tcaaacccct tttattaatg aggatattga gatgtggctg atacacgaac
    13261 cctggcgagg acagacaaat atgcggaagt atttaaatct ggtggcagaa aaaaaaataa
    13321 cgcctattga ttggggtaaa gatgggtggg gattagaaat agcagactct cttgagagga
    13381 cagatcaata tcctaaagat tacggtttat ccatgttaaa ttggcttttg ggtacttcac
    13441 ctgagccata tcttttacca tctcagataa tcgaaagata ttcgatattt gctaggccct
    13501 gaactttagg atctgttata catgagatga aaatatttaa ataaaggaat ttatatgaaa
    13561 gttgcctata ttctaaacac tccgaatgct gcaaattata aagtgggtac gatgattttg
    13621 cctcaattag aggaggatcg ccatggggtg gatgtgttag ggatattctt ttttgacgac
    13681 aatacatata tgttgagaaa gggagatcca aagggggaac gattggccac cattgcaaag
    13741 gagaagggaa tgctattaat gatgtgtgat caatgcgcta ttcaaaggga gcttgccaca
    13801 ggtgaagctg gtggtgccga acctaaggga acagttgatg gggtatctgt tggatgtttc
    13861 cccgatttat atgctgccct agcccctaac ccgccggacc agatcatttc tctttaattg
    13921 aacttaaagt tgtctcaacc ttgggtctaa cttgtcacga agccagtcac ccatgaagtt
    13981 aagagaaaga accaccaaaa atatggacat ccctggaaat attgctggcc accaggccgt
    14041 ctttagataa ccccttgctt cagctaccat aatgccccag gcaggggtgg gtgctggaac
    14101 tccagctccc aggaagctca aagctgcctc agctagaatg agggacccga cgctcaaggt
    14161 cgcgataacc actgcggtgt taattactcc tggtagtata tggcgtagca gaattctagg
    14221 gagagatgct cccgccactc ttgccaagtc cacgtaaggt tgatttttaa ttgtcagggt
    14281 ctgacttctt ataaccctga caaaaggatg ccaggctaac agaaccatca aagaaagcaa
    14341 tagggtagta ctttggccaa atatcatcac acacacgagt gcgaccataa ggaaaggtag
    14401 ggcctgccat atgtctacta tccgagtgat aagttcatct atccaacctc cagcgtaccc
    14461 acttacgata cctagagtgg ttccgattat gaagccgctc agcaaggcag cagccgcgac
    14521 agctagtgaa atcctggctc cgtaaagggt tcttgtgaag acatctcggc cagcatggtc
    14581 tgtacctaaa atatgttccg ttgtcccttc ttctgtccat gctggaggaa tatgcctgtc
    14641 tcttattcct ccttgcctag gatcctgagt ggccaggacg tcagccaata atccggataa
    14701 aattaataga ccaaggataa cgataggaat aacaggccaa gacctaatga aaatccatat
    14761 ggatctaagc tctctgacga ccggaggtct ttcatggagt ccaagttttt ttaaatcttc
    14821 ttcatgcaaa gccataaata ttaacctaac tgtaccttat tcttggatct ataaatgcat
    14881 aaagcaagtc agctataaat gcgaaggata catatattcc gataaaggaa aacacggttc
    14941 caacaagcag aggccagtca ttgtcgtaca ccgcctgatt gagtgctacc tggcctatac
    15001 caggccatga aaatactatt tctacgacta acgcaccgtt aagatagtcg gccagtagta
    15061 ctagcattga agtcaacggc ggtatgattg cgtttttcaa tccgtgtttc cagattaacg
    15121 gtagtcttgc tacacctttc gctctagcaa gttttatgta ttctgaatct agtatttcaa
    15181 gcattgatga ccgtgttagt cgcattatgc ctgcagccgc gggccatcct aataccatcc
    15241 atggcagtat ataaaatttg agattacccc
//
LOCUS       DASCNS010000068         9000 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000550462, whole genome shotgun sequence.
ACCESSION   DASCNS010000068 DASCNS010000000
VERSION     DASCNS010000068.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000550462"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /gene="nadA"
                     /locus_tag="QF838_02740"
     CDS             complement(<1..1175)
                     /gene="nadA"
                     /locus_tag="QF838_02740"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HJO59643.1"
                     /translation="MVIKIDKHPIPITEIEQSAFCQTDEELASKTLEQEFNSGVRWQK
                     LPVSYMRTSPEKLEQKISAAKKKLGNKIVILGHHYQRDEVIQFADVRGDSFKLSQHAA
                     ELRDPEYIIFCGVHFMAETADILSSQTQQVILPNLTAGCSMADMAHIDDVMDCWEDLT
                     EILGEGSVVPMTYMNSTAAIKSLCGKNGGIVCTSANASAAFNWAFEKGEKILFLPDQH
                     LGRNTAVTKGVNLNEMIVWNPFKPLGGNSIESIENSKIILWQGHCSVHTRFTVDQIKS
                     ARNSYADVSVVVHPECTLDVVESADYVGSTEYIRQIIDEGEPETTWAVGTEISLVNRI
                     ATENPDKNIFCLDSVVCPCSTMYRIHPAYLAWVLDGLLEGVVVNQITVDETTRKYSR"
     gene            complement(1193..2479)
                     /gene="miaB"
                     /locus_tag="QF838_02745"
     CDS             complement(1193..2479)
                     /gene="miaB"
                     /locus_tag="QF838_02745"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HJO59644.1"
                     /translation="MRSYHIWTTGCQMNKADSERLSSALNQMGLVSTESKEAADIVVL
                     NTCVVRQNAEDKAVGTLTSLKPSKELDPEKVIALMGCMVGPNMSALEKRFPYVDVFMA
                     PQQYSPLIKYLGEKLKIDAEGCIGPLSAAPAISTYIPIIHGCDKFCSFCIIPYRRGRE
                     KSRSVQEIVHETEMLVERGVKEVTLLGQNVDSFGHDLGGTINLSNLLVAVNEVSGLER
                     IRFLTSHPNDMEDSIIEAVNGLEKVCEHINLPFQAGDDEILRTMRRGYTNEEYRRLID
                     KIRNRVSGVSISTDLIVGFCGETDEQFANTLEMIKDIKFDKVHSAAYSTREGTIASRK
                     MIDDVEEDIKKERLKIINAEQEKIVACINSNLKGKTQNILVEGRKKGKWFGRNRNDKL
                     VFLDSDNVNAGEMIDVKIKETSPWYLEGSISNDIRK"
     gene            complement(2512..2603)
                     /locus_tag="QF838_02750"
     tRNA            complement(2512..2603)
                     /locus_tag="QF838_02750"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2567..2569),aa:Ser,seq:gct)
     gene            complement(2911..3360)
                     /locus_tag="QF838_02755"
     CDS             complement(2911..3360)
                     /locus_tag="QF838_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HJO59645.1"
                     /translation="MSKKIGYETFITHNELNIEKLSADVIADRHGAVVVFNGITRDHT
                     DGRKVLFLEYEAYLPMAKAKLNDVAEEMCDKWDVSVVIAHRIGRVDIGESSLIVTVGS
                     PHRASAYQASQYSVDRIKQIVPIWKKEHFDGGEVWIGDAEGFRPLTG"
     gene            complement(3378..3623)
                     /gene="moaD"
                     /locus_tag="QF838_02760"
     CDS             complement(3378..3623)
                     /gene="moaD"
                     /locus_tag="QF838_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HJO59646.1"
                     /translation="MKIKVLYFASYREQCGKNTETFTLPKESTLADLIACVLETNIGI
                     RCSPDTIVAAVNEEYQTHDQVLLAGDIVALIPPVSGG"
     gene            complement(3659..4408)
                     /gene="fabG"
                     /locus_tag="QF838_02765"
     CDS             complement(3659..4408)
                     /gene="fabG"
                     /locus_tag="QF838_02765"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HJO59647.1"
                     /translation="MQNNSSLNGKVALVTGSGRNIGRATALRLASLGAAVAVNARTNE
                     SEAKSVEKEITDNGGQSVAVLGNVGSESEVKSMFEKIEGTLGNVTILVNNAGLREDQF
                     TSQMTTGEWRRVLSVNLDGPFYCSRTAIPGMIEADWGRIINVSGLNSFKGKDGWGHVC
                     ASKMGAIGLTRALSIELAANNILVNHIVPGAFDTSNASGSNPTPVVTNIPVGRLGYPV
                     EIASAVDFLVSPENTFITGQTIHVNGGAFTY"
     gene            complement(4398..5459)
                     /locus_tag="QF838_02770"
     CDS             complement(4398..5459)
                     /locus_tag="QF838_02770"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HJO59648.1"
                     /translation="MKTAAIGVGAIAGTLAGFMAKEGNDVLLIDGWKEHVDSMNEKGL
                     TLNGIVGEHIVKVEAIHTDEIPNISDKFDMIIVGVKSYDTENAITAMLPFMNDETYVV
                     SPQNSINETLIAPLVGAHRTIGCITTISAGLYEPGHITRTGSVSQSLQKEPICFTVGE
                     LNGEITERVQTIADLFSSAGKTMVTDDLWGQRWSKMVTNCMVNATAGITGLLSHEVRA
                     DKETRNQILNLAIETVKVGRTLGYNVKPPMGDFSLEDMEKGAGPEGNEELDRVLQGTP
                     PEVPGRPSLAQDVMKGRKTEIDYLNGLVSDKGREIGVETPYSDAVTQVLKGVESGEFE
                     VGMDNIQRVSNIVRTYYAK"
     gene            complement(5545..6312)
                     /locus_tag="QF838_02775"
     CDS             complement(5545..6312)
                     /locus_tag="QF838_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJO59649.1"
                     /translation="MTYQTILYDTFEGVATITLNRPEVMNALSTTLYRELDRAITAVE
                     SDDEVKAVIITGAGDRAFTAGADIHEMTRNAESDNPPAQDPNRPTYAWHIGSCTKPTI
                     GALNGLAYGGGAVLASSLDIRIGCERTKFRFLAAAYGRVNSTWSLPMQIGWPKAKELL
                     LTARVVEAEESLQIGLINHLVHSTDLLDKANELAKMIVGNDDRMVQGIKKLLIEDIGE
                     GWESTYQKELTAQTNELKPTPVLEGFKPFIDRKGRKA"
     gene            complement(6404..6697)
                     /locus_tag="QF838_02780"
     CDS             complement(6404..6697)
                     /locus_tag="QF838_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015116257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HJO59650.1"
                     /translation="MDTYENIYEVVRQVPFGMVTTYGKVAQLSGMNNARVVGYALSVL
                     QANTEIPWHRIVNRKGAISYREGAGPSLQKTMLESEGVVFNKKGCINLEIYRW"
     gene            6834..8591
                     /gene="acsA"
                     /locus_tag="QF838_02785"
     CDS             6834..8591
                     /gene="acsA"
                     /locus_tag="QF838_02785"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HJO59651.1"
                     /translation="MAEANFKPIKKVSVDKMEIKPNLDLEESYKGFDWESLYKQLDWL
                     PGGGLNKAHEAIDRHANGDKREKIAMIWEGKNGEREDYTFGDMQRLTNKFANVLQSLG
                     IQKGDRVFIFMDRLPELYIAFFGALKAGAVVGPLFSAFGPEPVRDRMQDSGAKVLVTQ
                     PDLRKKIAEIIPELFDLQHILIVNKDFRDAAPIDPQDLSYDEEMDKASEDFTTVVTGQ
                     YDYSIMHYTSGTTGKPKGAVHRHQAVVQQMATGRWALDLHDDDIYWCTADPGWVTGTS
                     YGMLAPWTNGVTQLIYEGGFSASRWYEQIQKHKVTVWYTAPTAIRMLMKAGEKVPAKF
                     ELSSLRYMCSVGEPLNPEAVVWGDDVFGMPFHDNWWQTETGAIMCANYPSMEVRPGSM
                     GKPIPGVQLAIVDEDFNEVPDGEDGNLVVKPGWPSMFHGYWNNAEMYNSRFRKGWYIT
                     GDTARKDSDGYFWFVGRADDVINTAGHLVGPFEVESALIEHPAVAEAGVIGKPDEIAM
                     EIVKAFVSLKDGYEESTKLRNEIMRFAREKLSTAVAPREIDFIPSLPKTRSGKIMRRL
                     LKARELGLPEGDTSTLEDD"
     gene            8632..>9000
                     /locus_tag="QF838_02790"
     CDS             8632..>9000
                     /locus_tag="QF838_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJO59652.1"
                     /translation="MDLERIKSLVCDEIERNGDKLISIAKEILKNPEPGFKEFKTAEI
                     VSREFEAFQIPHEKGIAITGLKSSLETGTPGPNIAVIGELDSLKVLGHPYEDKETTAA
                     HACGHHCQIAMMLGVAVGLKA"
BASE COUNT         2362 a         1975 c         1836 g         2827 t
ORIGIN      
        1 atccgtgaat attttctagt ggtctcatct actgttatct ggtttaccac gacgccttct
       61 agtagcccat ccaagaccca ggctagatag gctggatgaa tccgatacat tgtagagcaa
      121 gggcagacaa ctgaatccag gcaaaaaata tttttatccg gattttcagt ggcaattctg
      181 ttcactaaac ttatttcagt gcctaccgcc caagtcgttt cgggctctcc ttcatcaatt
      241 atttgcctta tgtattctgt ggagcctaca taatctgctg attcgactac atctaatgtg
      301 cattctggat gtaccacgac ggagacatcg gcataagagt ttctggcaga ttttatttga
      361 tcaaccgtaa atctggtgtg aactgaacaa tggccttgcc atagaatgat tttagaattt
      421 tcaatcgact caattgaatt cccccccaaa ggtttaaaag gattccaaac gatcatttca
      481 ttaaggttta cacctttcgt tacagcagta tttcttccca aatgttgatc tggaaggaat
      541 aggattttct cccctttttc aaaagcccaa ttgaaggcag ctgaagcatt tgcagatgta
      601 caaacgatcc caccattttt tccacagagt gattttattg cagcggtgga gttcatatag
      661 gtcattggta caacggaccc ttcgcccagt atttctgtga gatcctccca acaatccatt
      721 acgtcatcaa tatgagccat gtcagccatg gaacacccag cggtgaggtt gggtaggatg
      781 acttgttgtg tttggctact taatatgtca gcagtttcgg ccatgaaatg aacaccacaa
      841 aaaataatat attcggggtc tcttaattcc gcagcatgtt gtgataattt gaaagagtca
      901 cccctaacat ccgcaaactg aatgacctca tccctttgat aatggtgacc aagtattacg
      961 attttattac caagcttctt ttttgctgca gaaatctttt gttcaagttt ttccgggctc
     1021 gttcgcatat aacttactgg aagtttttgc caacgaacac cggaattaaa ctcctgttct
     1081 aaagtttttg aggctagttc ttcgtcagtt tgacagaagg ccgattgttc tatttctgta
     1141 attggtatgg ggtgtttatc tatttttata accatcttct atgtattcct tgttactttc
     1201 ggatgtcgtt acttatagag ccttccaaat accatgggct cgtctctttt attttcacgt
     1261 cgatcatttc gccggcattc acattgtcac tatcaagaaa aaccagctta tcattcctgt
     1321 ttcttccaaa ccatttccct ttttttcgtc cctcgacaag gatgttctga gtctttcctt
     1381 tgaggttgga attgatgcag gccactattt tttcttgttc ggcattaatt attttgaggc
     1441 gctctttttt tatgtcttct tctacgtcgt caatcatttt ccttgaagct atagtacctt
     1501 ctcgggtgga gtacgccgcc gaatgaacct tgtcgaattt tatgtcttta atcatctcaa
     1561 gagtgtttgc aaattgttcg tcagtctccc cgcagaaacc aactattaaa tctgtgctta
     1621 tactcacacc ggacacccta ttcctaattt tatctattag gcgcctatat tcttcgtttg
     1681 tgtaaccgcg tcgcatagtg cgcagaatct cgtcatcccc ggcctgaaaa ggtaaattta
     1741 tatgctcgca aactttttct agaccattca cggcttctat tatgctgtct tccatatcat
     1801 tgggatgtga ggttaagaat cttattcttt ccaagccaga tacttcattt acagctacca
     1861 acaagtttga aaggttaatt gttcccccta gatcgtgacc aaaggaatct acattttggc
     1921 ctagcaatgt aacttcctta actccccttt ctaccaacat ttcggtttca tgaacaatct
     1981 cttgtacaga tctacttttc tcccttccac gacgataagg aataatacaa aatgagcaaa
     2041 atttgtcaca cccatgaatt attgggatgt aggtgcttat tgcgggagca gcgcttaaag
     2101 gacctataca gccttccgca tcaattttta acttttctcc gagatattta atcagtgggc
     2161 tgtactgctg gggtgccata aatacatcta cgtaagggaa acgtttttct aaagcactca
     2221 tgttgggccc aaccatacac cccatcagag caataacttt ttctggatcc agctctttac
     2281 tcggctttaa gctggtcaaa gttcctacgg ctttgtcttc agcgttctgc cggacaacgc
     2341 atgtgttaag cacaactatg tcggctgctt cttttgattc cgttgacaca aggcccatct
     2401 gatttaaagc actacttagg cgctctgaat ctgccttgtt catttggcat cctgtagtcc
     2461 aaatgtgata gcttcgcatc tatttattgc aagccccata attttcagtt gtggcggagg
     2521 gaatgggatt cgaacccatg agaggacttt catcccctaa ccgcttagca ggcggccgca
     2581 ctagacctct atgcgatccc tccaattcat ttatagaact tcaattatag gtttgactat
     2641 ctagtcgtgg caagacaatt aattgtgaaa tccggaacta atctatatat atatacgaat
     2701 accgtaaaaa aattagaaaa tacgcgaatt aacacatagg gtaattgtat aaattatacg
     2761 ataaacgtac tttaacctaa tgtgtatgcc gaaatccccg aaaatgtact ttcataggaa
     2821 aagtaatgaa ttaggaatgc ttttaccttt ctttggtcgc gcccaggtaa atattttcct
     2881 atattttctg ccattaaatt tagagtacgt ttatccagtc agtggtctga atccttctgc
     2941 atcgccaatc cagacttctc cgccatcaaa atgctccttt ttccagatag gaactatttg
     3001 ctttattcta tcgactgaat attggctggc ttgataagca gatgctctgt gtggggaccc
     3061 aactgtaact attaggctag attcccctat atctactctg ccaattctgt gggctatgac
     3121 taccgatacg tcccatttgt cacacatttc ctcggcgacg tcgttaagtt tcgctttggc
     3181 cattggaagg taggcttcat actcgaggaa caaaactttt cttccatctg tatgatctct
     3241 ggttattcca ttgaaaacca caacagcacc gtggcgatct gcaatgacat ctgctgataa
     3301 tttttcaatg ttcaactcat tatgggttat gaatgtttcg tatccaatct tcttagacaa
     3361 actggctcct ggttgattta tccaccactc acagggggta tcagcgccac aatatctcct
     3421 gcaagcagca cctggtcgtg tgtttgatac tcttcattca ccgctgcaac tattgtgtca
     3481 ggggagcatc ttattcctat atttgtttct aaaacacaag caataagatc agccaacgta
     3541 ctttctttag gcaatgtaaa tgtttctgta tttttcccac attgttctct gtaggatgcg
     3601 aaataaagta ctttaatttt cagtttgaat ctccgaaata gtcagtattc gtctctgctt
     3661 aataagtaaa tgccccgcca tttacatgta ttgtttgccc ggtgatgaag gtgttttcag
     3721 gggataccaa aaaatctact gccgaggcta tctcaactgg atatccgagt cggccaacag
     3781 gtatgtttgt aacgaccggt gtcggattgc ttccggatgc attggaagtg tcaaacgcac
     3841 ctgggactat gtggttgacc aatatgttat ttgctgcaag ttcgattgat aaagctcgtg
     3901 ttaaccctat cgctcccatt ttggaagcac agacgtgtcc ccatccatcc tttcctttaa
     3961 atgaatttaa gccagataca tttattatcc ttccccaatc agcttctatc attcctggta
     4021 tagctgttcg gctacagtaa aatgggccat caaggtttac ggataaaacc ctacgccatt
     4081 ccccggttgt catttgggaa gtaaactgat cttctctgag ccctgcgtta tttaccagta
     4141 tggtgacatt tcctaatgtt ccttcaattt tttcaaacat tgatttcact tcactctctg
     4201 atccaacgtt tcccaacact gcaacggact gcccaccatt gtctgtaatt tctttctcca
     4261 ccgacttagc ttctgattca tttgttctgg catttactgc cactgcagcg cccaaagatg
     4321 ccagtcgcaa tgcggttgcc cttcctatat tccggccgga accggttact aaagccactt
     4381 tcccgtttaa agatgaatta ttttgcataa tatgtcctta caatgtttga aactctttgt
     4441 atattatcca tacccacctc aaattccccc gattcaactc cctttagcac ctgggtaact
     4501 gcatcactat agggggtttc tacacctatt tctctacctt tatcagacac gaggccattc
     4561 agataatcaa tttcggtttt tcttcctttc attacatctt gggctaagga tggtcttcct
     4621 ggcacttcgg gtggagttcc ttgtaggact ctatctagtt cttcatttcc ttctggaccg
     4681 gcgccttttt ccatatcttc aagtgaaaag tcgcccatgg gaggtttaac attgtaccca
     4741 agagttcgac cgacttttac cgtttcaata gctaaattga gtatttgatt tctggtttct
     4801 ttatcggctc tcacctcgtg actcaaaagc ccagttatcc ctgccgttgc attgaccata
     4861 cagttagtga ccatttttga ccagcgttga ccccacagat cgtccgtaac catcgtttta
     4921 ccagcagaac tgaatagatc cgctattgtc tggactcttt cggtgatttc tccgttcaat
     4981 tctcctactg tgaagcaaat tggttctttc tgtagagatt gagaaacact gccagtgcgg
     5041 gttatgtggc ccggctcgta aagccctgca gaaatagtag ttatgcatcc gatagttcta
     5101 tgagctccta caagaggggc tatcaaagtt tcgttaatgc tattttgagg gcttaccaca
     5161 taggtttcgt cattcatgaa tggaagcatc gccgtgatag cattctccgt gtcgtaggat
     5221 tttacgccta caataatcat atcgaattta tcacttatgt ttgggatttc atctgtgtgt
     5281 atagcctcga ccttgacgat atgctctccc actatgccgt tcaaggtaag tcctttttca
     5341 ttcatggaat ccacatgttc tttccatcca tctatcagta acacatcatt gccttcctta
     5401 gccataaaac cagctaaggt tcctgcgatt gctcctactc cgatagctgc cgttttcata
     5461 cctaattctc cttacttaaa tcggcgtcga actttattct gttctcataa tttgatatat
     5521 aaccagcgat ttttcgatat tgacctaagc ttttctgcct ttgcggtcta taaaaggttt
     5581 aaatccttct aagactggtg taggtttaag ttcattggtt tgagcagtca actccttttg
     5641 ataggtactc tcccagcctt cccctatgtc ttcgatcaaa agtttcttga tcccttgcac
     5701 catgcgatcg tcgttcccta caatcatttt ggccaattcg ttggctttat ctaagaggtc
     5761 agtagagtgg actagatgat ttatcaatcc aatttggaga ctctcttcgg cttctacaac
     5821 acgggcagtg agtagcaatt ccttggcttt aggccaacct atttgcatag gtaagctcca
     5881 cgttgaattt actctcccat atgcggctgc aagaaatcta aattttgttc tttcgcaccc
     5941 aattcttata tccaaacttg aagccaaaac agcacctcct ccgtaggcca aaccgtttag
     6001 ggcccctatg gttggttttg tgcagcttcc aatgtgccag gcataagtag gtctgttagg
     6061 gtcttgggca ggaggattgt cactttcggc atttcttgtc atctcgtgga tgtctgcccc
     6121 tgcagtgaat gctctgtctc cagcaccagt gattataact gccttgacct catcgtcaga
     6181 ttcgaccgct gttatggctc ggtcaagttc acgataaaga gttgtgctga gggcattcat
     6241 tacctcgggt ctattgaggg ttatagtagc taccccttca aatgtgtcat ataggatcgt
     6301 ttgataggtc aatacatgct ccttcagttc aattgttagt ggtgaagaat tataggaagc
     6361 ttttttccaa agggtcaaga aatcggatgt aggatattga gcactaccat cgatatattt
     6421 ccaagttgat gcaacccttt ttgttaaata ctactccctc tgattccagc atagtttttt
     6481 gcaaacttgg ccctgctccc tctctgtaac ttatcgctcc ctttctgttc acaattctgt
     6541 gccatggaat ttctgtgttg gcttggagga cagaaagggc gtaaccgacc accctggcat
     6601 tattcatccc tgaaagttgc gcgactttac cgtatgtggt caccatacca aacgggacct
     6661 gtctaactac ttcatatata ttttcatagg tatccatgcg aaatatctca aaatattggt
     6721 tgtaaatatt atttccacta tcttctttca ggccaaaaat aaaaagttag cactataaac
     6781 catgatcctg ataatattcg cattctacac aaatgattca cgaggttagt tttatggcag
     6841 aggcaaattt caaaccgata aagaaagttt cagtggataa aatggagatt aagccgaatc
     6901 tcgacctcga agaatcttat aaaggttttg attgggaatc cctttacaaa cagttggatt
     6961 ggctgcccgg aggaggcttg aacaaagctc atgaagctat tgatcgacat gctaatggtg
     7021 ataaacgaga gaaaatagct atgatttggg aaggtaaaaa cggggagcgt gaagattata
     7081 cctttggtga tatgcagcgc ctgactaata aatttgcaaa tgtactgcaa agtcttggaa
     7141 ttcaaaaagg tgaccgggta ttcatattta tggataggct gcctgagctc tatattgcat
     7201 ttttcggggc tttgaaggca ggtgctgttg tagggcctct tttctctgct tttggtccgg
     7261 aaccagttag agataggatg caagatagtg gggctaaagt gttagttacc cagccggact
     7321 tgagaaaaaa aattgctgag atcatcccag aattatttga ccttcagcac atattaatag
     7381 tgaataaaga ttttagagat gctgctccta ttgaccctca ggacctttct tatgatgagg
     7441 aaatggataa ggcttctgaa gatttcacca ctgtagttac aggtcaatac gattattcca
     7501 taatgcatta cacctccgga actaccggga aacccaaagg tgcagttcac aggcatcaag
     7561 ctgtagtgca gcaaatggcc acgggtcgat gggcattgga tctgcatgac gatgacattt
     7621 attggtgcac agcggaccca gggtgggtga ctgggacttc atacgggatg ctagcacctt
     7681 ggacaaatgg tgtaactcag ttgatttacg agggaggatt cagtgctagt agatggtacg
     7741 aacagataca aaagcataaa gtcactgttt ggtatacggc tccaactgcc attcgaatgc
     7801 ttatgaaggc gggagaaaaa gtcccggcca agtttgagct ctcttcattg aggtacatgt
     7861 gtagtgtagg tgaaccatta aaccctgagg cggttgtgtg gggggacgat gttttcggaa
     7921 tgcctttcca tgacaattgg tggcaaacag agactggggc aattatgtgt gccaattatc
     7981 caagtatgga agtgaggcct gggtctatgg gtaaacctat accaggtgtt cagcttgcca
     8041 tagtagatga agatttcaac gaggtgccag atggagaaga cgggaacttg gtcgttaaac
     8101 ccggatggcc gtcaatgttt catggatatt ggaacaatgc tgaaatgtat aattccagat
     8161 ttagaaaagg gtggtatata accggagaca ccgctaggaa agattctgac ggatatttct
     8221 ggtttgttgg aagggctgat gatgtaataa ataccgctgg ccatctcgtc ggaccctttg
     8281 aagttgaaag cgctttgatt gagcatccgg cagtggctga ggccggtgta attgggaaac
     8341 cagacgaaat tgctatggag atagttaagg catttgtatc tcttaaagat gggtatgaag
     8401 aatccactaa acttagaaat gagatcatgc ggttcgccag agagaaacta tcaaccgcag
     8461 tagctcctcg ggaaatagat ttcataccgt cattgccaaa aactaggagt ggaaagatca
     8521 tgcgtcgact tttaaaggct cgcgagcttg gactaccaga aggagatact tcaaccctag
     8581 aagatgatta attttatatc aactggttaa tttaatgagg agtgttggat tttggatcta
     8641 gaaagaatta aatctttggt ttgcgacgaa attgaaagaa atggggataa attaatttcc
     8701 attgccaaag agatactgaa aaaccctgag ccagggttca aggaatttaa gactgcggaa
     8761 atcgtttctc gggaatttga agcttttcag ataccccatg agaaaggtat agcaattaca
     8821 ggtcttaaat cttccttaga aactgggacc cctggtccaa acatagccgt cattggtgaa
     8881 ttagattcac ttaaagtact gggacaccca tatgaggata aagaaactac agctgcgcac
     8941 gcctgcgggc atcattgtca gatagccatg atgttgggtg tcgctgtcgg attgaaggca
//
LOCUS       DASCNS010000069         3397 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000551325, whole genome shotgun sequence.
ACCESSION   DASCNS010000069 DASCNS010000000
VERSION     DASCNS010000069.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000551325"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="QF838_02795"
     CDS             complement(<1..195)
                     /locus_tag="QF838_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HJO59653.1"
                     /translation="MTEKYILAIDQGTTGTTVALVNSRGGLYATSYKEITQFYPNPGW
                     VEHDPQEIIESVFFCIREVMK"
     gene            299..679
                     /locus_tag="QF838_02800"
     CDS             299..679
                     /locus_tag="QF838_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HJO59654.1"
                     /translation="MNERSITINAGNLSLDGTLNESDMAEQIWNSLPLQGTANIWGDE
                     IYFAIPVSTTEHLGASDLVSSGDLAFWPPGNAFCIFFGRTPASTDDQPRAASAVNVFG
                     HISGDEKLFRGVKGGTDISIVAKG"
     gene            692..1354
                     /gene="tenA"
                     /locus_tag="QF838_02805"
     CDS             692..1354
                     /gene="tenA"
                     /locus_tag="QF838_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HJO59655.1"
                     /translation="MSYTKLLREEADHLWEKEYMHPFVQGIGDGTLTLEKFQHYMKQD
                     YLFLIEYSKVISLAIAKSKNLDDMGWFATLLNETLNTEMALHVSFCQDFGIPVEDLLV
                     TKMSPTTHAYTRHLLEVGYSGSTPEIATAILPCSWGYSEIGKRLFEKGVPQNNPLYSR
                     WIQMYNSDEFEELALWVRNFIDREAITMTTANQETLKDIFFTSTRYEYNFWNSAYALE
                     TW"
     gene            1473..1736
                     /locus_tag="QF838_02810"
     CDS             1473..1736
                     /locus_tag="QF838_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59656.1"
                     /translation="MAKIDSIDVAEPVNEKTCFHHWMIGDPDGPTSSGKCKLCGTQRD
                     FMNYFEGSSWGSDIALDQLGKSTGATREASYVSVTPTSKDEEE"
     gene            complement(1737..2498)
                     /gene="cofE"
                     /locus_tag="QF838_02815"
     CDS             complement(1737..2498)
                     /gene="cofE"
                     /locus_tag="QF838_02815"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HJO59657.1"
                     /translation="MGNIEIIGLEGLPEFDTSHDLPKLIYESALSSAGGMKSGDVIVI
                     TQKIISKVEGMVRDLLNVIPTPEALELADKLGKDPRLVQMILEQSSEIIRTDFERGVL
                     ITESIHGFICANAGIDSSNIEGDTKIALLPRDSNVSAKQILKSLKNLSGIDSIGVIIS
                     DTFGRPWRDGHVNFAIGCAGMSPYINYKDTVDAVGKVLKVTTIAVADELAAASELVTG
                     KSLNIPAVLFRGVEFKPESAEPESLLRPKENDLFR"
     gene            2637..>3397
                     /locus_tag="QF838_02820"
     CDS             2637..>3397
                     /locus_tag="QF838_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HJO59658.1"
                     /translation="MLNKAASFSPDAFVPDMEDSVAWDDKSQAREIVSANLGMLANTG
                     KHVIPRINSLDTGLCETDLEAIVGPHIYGVSVGKINTPADVVVISETIARLETEHNIT
                     KGSIKLVLWIETAVGILNAHQILSSDDRIVAAAFGAEDFTNDMGIERNEDDREILFPR
                     SQVAIAARAAGVLALDTPFFGFRDPDSLKLNSEQSKSIGFKGKFAIHPLQVEIINQCF
                     SPSKNEISHAMEVIKVFEEAAASGRGSTSLNGQVV"
BASE COUNT         1013 a          697 c          702 g          985 t
ORIGIN      
        1 cttcataacc tcacgtatgc agaaaaacac actttctatt atttcttggg ggtcatgttc
       61 tacccatccc ggatttgggt aaaattgggt tatctcttta tagctggttg catataatcc
      121 tccacgagaa ttcaccaagg ctactgtcgt accagtggtc ccttgatcga ttgcaagaat
      181 atatttttct gtcatatgca tcactccgtc ctaaagttga aaatttaggt ggaaccatat
      241 ggtatggtga agccggagta tgtcaacttt tttctaccag cagtgaatag gaggtttcat
      301 gaacgaacga tctataacga taaatgccgg taatctttcg cttgatggga ccttgaatga
      361 atcagacatg gcggaacaaa tctggaattc cttaccatta caaggaaccg caaacatatg
      421 gggagatgaa atatactttg ccattcctgt cagtactaca gagcatttag gggcctcaga
      481 tttagtgtca tcaggagatc ttgcattttg gcctccaggc aatgcttttt gtatattctt
      541 tggacggact cctgcaagta ccgatgatca accacgagct gctagtgcag tgaatgtatt
      601 tggtcatatc agcggtgacg agaagctatt cagaggggtg aaaggcggaa cagacatcag
      661 tattgtggct aaaggataat cggatattca aatgagttac acaaagctac ttcgagaaga
      721 agcggatcac ctttgggaaa aggaatacat gcaccccttt gtacaaggaa taggagatgg
      781 cactctcact cttgagaaat ttcaacacta tatgaaacaa gattatttat ttctcattga
      841 atattctaaa gtcatttctc ttgccattgc aaaatcaaaa aaccttgacg acatgggttg
      901 gtttgccaca ctacttaatg aaaccttgaa tactgaaatg gctcttcatg tcagtttttg
      961 ccaagatttc ggtatcccgg tagaagacct tttagtgacc aaaatgtcgc cgaccaccca
     1021 cgcatacaca agacatcttt tggaggtagg atattcggga tcaacacctg agatagccac
     1081 tgctatattg ccatgttcat ggggttattc ggaaattggg aaacgtttat ttgaaaaagg
     1141 ggtcccacag aataatcctc tatattcaag atggatccaa atgtacaact ctgatgaatt
     1201 tgaggaattg gccttgtggg tccgtaattt tattgaccga gaagcaataa ccatgactac
     1261 agctaaccaa gaaaccctaa aagatatttt cttcactagt actcgatacg aatataattt
     1321 ctggaattct gcctacgctt tagagacttg gtgaagatac gtaactaacc ctatattact
     1381 ataaatgata atatttctat aatttatcat cgtatggtat acttatgcct ataaatttat
     1441 gtaatcatta aagaacagca gaggcggtta ctgtggccaa gatagattcg atagatgtag
     1501 ctgaacccgt aaatgaaaaa acatgctttc accattggat gatcggtgat cccgacgggc
     1561 ctactagttc aggtaaatgc aaactttgtg gtactcaaag ggacttcatg aattattttg
     1621 aaggttcatc ttggggctct gatattgcct tagaccagtt aggaaaatct actggtgcca
     1681 ccagagaagc atcttatgtc agtgtaaccc cgaccagtaa agatgaggaa gaataactaa
     1741 cgaaatagat cgttctcctt tggtctcagt agggactcag gctccgcgct ttctggcttg
     1801 aactctactc ctctaaacaa aaccgctgga atgtttaagg actttccagt tactaattca
     1861 gaggctgcgg ctaactcatc ggcaactgct atcgtggtta ctttaagtac cttaccaact
     1921 gcatccacag tatctttgta atttatataa gggctcattc ctgcgcagcc aatggcaaaa
     1981 tttacgtgcc catccctcca aggacggcca aatgtgtctg aaattatgac accgatggag
     2041 tctatacctg ataaattttt cagacttttg agaatttgct ttgcggaaac attggagtct
     2101 ctaggtaaaa gggcaatttt agtatctcct tctatgttag aagaatcaat gcctgcgttg
     2161 gcacaaatga aaccatggat actctcagtt atcaatactc ccctttcaaa gtctgttctt
     2221 atgatttctg atgattgctc caaaatcatt tgaaccaacc ttgggtcttt tccaagttta
     2281 tcagccaact ccaaggcttc aggggtagga atgacattta gtaggtcacg aaccattcct
     2341 tcaactttgg aaatgatttt ctgcgtaatg actatcacat caccggattt catccctcca
     2401 gccgaggaga gggcagattc ataaatcaac tttggcaaat catgtgaagt atcgaattca
     2461 ggaagaccct ctagacctat aatttctata tttcccatta gttaatatcc cctaaattct
     2521 taatgttata ctcccgaaag cattatgatg ttaatttcca atattctcgt aagtgggagg
     2581 tttttgcttt gctttatctg agaagtttac tatttgtccc tggaaaccag acaaacatgt
     2641 taaacaaggc cgcctcattt tctcctgatg ctttcgttcc agatatggaa gattctgtag
     2701 catgggacga taaatcacaa gcgcgagaaa tcgtctcagc caatctaggg atgctagcaa
     2761 atacgggtaa acacgttatt cctagaataa attcgttgga tacaggcctt tgcgaaactg
     2821 atttagaggc tatagtcggg ccacacatat atggcgtgtc tgtcggcaaa atcaacacgc
     2881 ccgctgatgt ggtagtaatc tcagaaacta ttgctcgatt ggaaactgag cacaatataa
     2941 cgaaaggatc tatcaagcta gtgctctgga tagagacggc agtaggcatt ttgaacgccc
     3001 atcaaatatt atcttctgat gaccgtatag tagccgccgc atttggagca gaagatttca
     3061 ccaacgatat gggaattgaa cgaaatgaag acgaccgtga aatacttttc ccgcgaagtc
     3121 aggtagctat agccgctagg gccgctggcg ttttggcctt agacacccca tttttcggct
     3181 ttagagatcc agatagtttg aaattgaatt ccgaacaatc taaatccatc ggctttaaag
     3241 gaaaattcgc aatacatcca ttgcaggtag aaataattaa ccagtgtttc tcaccatcca
     3301 agaacgaaat atcgcatgcc atggaagtta ttaaagtctt cgaggaagca gcagcaagtg
     3361 gcagaggttc gacttctttg aacggtcaag tggtgga
//
LOCUS       DASCNS010000070         1254 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000554514, whole genome shotgun sequence.
ACCESSION   DASCNS010000070 DASCNS010000000
VERSION     DASCNS010000070.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000554514"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="QF838_02825"
     CDS             <1..730
                     /locus_tag="QF838_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HJO59659.1"
                     /translation="IVGMEKIIPSMEDLGTFLRLLIRSATGQWISSYVTMVSGPREST
                     DIDGPEEFHLVIVDNGRSKMLADPYLREALYCLRCGACLNACPVYRKVGGHSYGWVYP
                     GPIGSIVSPILTGLSDAKNLPFASSLCGACKEACPVKIDIPRMLLYLRNQLAEGKDYP
                     DQQSAGLGERVVSKFLSSLLSNNKAIGFLVRSANLIATIAPFVRKPFPPSWTKSRESP
                     TLAKKTFVDQWVSLDLDGSLRKKH"
     gene            complement(737..>1254)
                     /locus_tag="QF838_02830"
     CDS             complement(737..>1254)
                     /locus_tag="QF838_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59660.1"
                     /translation="PEDGDDLIIRPSESYDNATPSGSSMTAEALFKLSLITNKSEYFN
                     IFISLMSSTATIAGEHPVSFGNWLCVYDSYIYPTIEIAIISDNAKQALKMRDCLYESY
                     VPNHIFCGNAYFSHTEKARNKWDTYPLLNDREPKQDIAMAYLCENYVCELPVSSTTEL
                     LRLIRQKTKVS"
BASE COUNT          333 a          229 c          302 g          390 t
ORIGIN      
        1 tattgttggc atggaaaaaa ttattccatc tatggaagat ttgggcacat ttttgcggtt
       61 gcttataagg tcggcaacgg gccagtggat ctcaagttat gtgaccatgg tctcgggacc
      121 acgggaaagt actgatatag acggtccaga agagttccac ttggtcatcg tagacaatgg
      181 gagatctaag atgctggccg atccctattt gagagaggca ttatattgcc tgagatgcgg
      241 ggcatgttta aacgcatgcc ctgtttacag gaaagttggg ggacattcct acggttgggt
      301 ttatccgggg ccaatcgggt ctattgtgtc ccctattttg accgggttgt cagatgctaa
      361 gaatttgcct tttgcctcca gtttgtgtgg ggcttgtaaa gaggcatgtc cggtgaaaat
      421 agatattcct aggatgcttt tgtatttgcg taatcagctt gcggaaggta aagattatcc
      481 agatcagcag tccgctggac taggggagcg agttgtttct aaatttttgt cttctttgtt
      541 atcaaataat aaggcaattg ggtttctcgt acgatcggcc aacttgattg caaccatagc
      601 cccttttgtt cgaaagccat tcccgccttc gtggacgaaa agtagggaat cccctacatt
      661 ggcaaaaaag acatttgtag atcaatgggt ttctttagat ttggacgggt ctttacgtaa
      721 aaagcattaa ctcgatttag gacaccttcg tcttttgtcg tattaaccgc aatagctctg
      781 ttgttgagct gacaggaagt tcgcaaacat aattttcgca taaataagcc attgctatat
      841 cttgtttcgg ttcgcggtca ttaagtaggg gataggtatc ccatttgttc ctagcttttt
      901 ctgtgtggct gaaataggcg ttcccacaaa aaatatgatt agggacatag gactcgtaca
      961 aacaatcacg cattttcaaa gcttgttttg cattatctga aataattgct atttcgatgg
     1021 ttggataaat ataagaatcg taaacacata accaattacc gaaagagact ggatgctcac
     1081 ctgcgatggt ggcagtagag ctcattaatg atatgaatat attaaaatat tctgatttgt
     1141 tagttatcag ggataattta aataatgcct ccgcagtcat ggatgagcct gagggtgtgg
     1201 cgttatcgta actttcacta ggtctaataa tgaggtcatc accgtcttct ggag
//
LOCUS       DASCNS010000071         1133 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000564759, whole genome shotgun sequence.
ACCESSION   DASCNS010000071 DASCNS010000000
VERSION     DASCNS010000071.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000564759"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="QF838_02835"
     CDS             <1..950
                     /locus_tag="QF838_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO59661.1"
                     /translation="LTSLAEFLQAPVIATGEGRGAISDRSYLSIGSFRFKNDTFFENK
                     IQDYDLVVAVGTRLLNNQQFTGQKVLQIDIDDEEIGRNYDNTEGINGDAKKTLTEILN
                     GLKNVMPPRESREEELTKVKAERFNPGTVIEPQESFTKVIRSVMPDDGIFISGMTQIG
                     YYSRNKFEVYEPRTYITSSYYGNLGYAYPTALGAKVAQPDKAVVAVCGDGGFMFNVQE
                     LATAAKHHINVVAVVFNDSAYGNVMRDQVNMFDGREYGAEVHNPDFMKLAEAFGVRGV
                     RVEGGAPELGKAVQESLGIEAPTLIEVPVGPMPNPFRDY"
BASE COUNT          368 a          227 c          260 g          278 t
ORIGIN      
        1 aactcacatc tttagcggaa tttctacaag cccctgttat agcaaccggt gaaggtagag
       61 gggctatttc agacagaagt tacctatcga ttggtagttt ccggttcaaa aacgacacct
      121 tttttgaaaa caaaatccaa gactatgacc tggtcgtagc ggttggaaca aggttattga
      181 acaaccaaca attcacaggg caaaaagtcc tgcaaatcga catagacgat gaagaaatcg
      241 gtcgtaatta tgacaacaca gaaggaataa atggcgacgc caaaaagact ctcacagaga
      301 ttctaaatgg actaaaaaac gtgatgccgc ctcgtgaaag cagagaagaa gaactcacaa
      361 aggtaaaggc cgaacgattt aatcctggca ctgtaattga gccgcaagaa tcttttacca
      421 aagtcattcg aagtgtcatg ccagatgatg gaatattcat ctccggcatg acgcaaattg
      481 gatattacag taggaataaa tttgaagttt atgaaccgag gacctacatt acctcctcct
      541 attacggtaa ccttggatat gcttatccaa ccgcattagg cgctaaagtt gcacaaccag
      601 ataaggcagt cgttgctgtt tgtggggatg gtggatttat gttcaatgta caagaattag
      661 ccacagctgc aaaacaccac attaacgtgg ttgctgttgt gtttaacgat agcgcttacg
      721 gaaatgtaat gagagatcag gtcaacatgt ttgatggaag agaatatgga gctgaggttc
      781 ataacccaga tttcatgaag ctcgctgaag cttttggtgt aaggggtgta agagtcgaag
      841 gtggagcacc tgaattagga aaagctgtgc aagagtccct gggtatagag gctccgacat
      901 tgatagaggt accagtaggg ccaatgccta atcctttcag ggattactaa acaatttcgt
      961 cagggcccaa taaaggagcc ctgacgagta tatctacact aaaccaggga aaagggaata
     1021 gacaattcac cgcatatatc tttaaaccag tctataacct cataatgtaa gggtatacca
     1081 ttttcagatc tttcaatcgc tgcctcatgc tcgggctggc ccggatacat aac
//
LOCUS       DASCNS010000072         1779 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000565900, whole genome shotgun sequence.
ACCESSION   DASCNS010000072 DASCNS010000000
VERSION     DASCNS010000072.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000565900"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(4..1173)
                     /locus_tag="QF838_02840"
     CDS             complement(4..1173)
                     /locus_tag="QF838_02840"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HJO59662.1"
                     /translation="MRSYLKNLECTYCNEIFSADQPRRLCEKCGKVLYPRYDMKEASK
                     YFTKDMLSARAPNMWRYFEMLPVYDESNIVTLGEGFTPIFETKRLGSELGSESLMLKD
                     EGLNPTASFKARGLSAAVSKAKELGLRKLTMPSAGNAAGAMASYAARAGMEAFVFMPQ
                     DAPEANQKEVSVSGGNLFLIDGLINDAGAISREKAAEIGLFDVSTLQEPYRVEGKKTM
                     GYEIAEQLDWELPDNIIYPTGGGTGIVGMWKAFQEMEDLEWISSKRPKMFAVQAENCA
                     PIVRAFQNGDEFAQPWENATTLAAGMRVPSAIGDYLILNAIRDSGGSAITVSDEEMTD
                     MTDLIGKLEGFFVCPEGAATACGARKLIKTGSIDPSEKTLLLNTGSGLKYLDMLP"
     gene            1289..>1779
                     /locus_tag="QF838_02845"
     CDS             1289..>1779
                     /locus_tag="QF838_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJO59663.1"
                     /translation="MADYDVIIIGGGAAGFSAGMYSSRLGMKTLLVERLIPGGQIINV
                     ERIEDYPGLLEGISGADFSSKVQEQAIASGTEFAMSEVTGIRKSGKEWIVETYEGQKT
                     SKSVIIAGGSTLDKLNVPGEDELVGAGVSYCATCDGAFFIDQNVCVVGGGDSALQEAI
                     TLT"
BASE COUNT          506 a          405 c          352 g          516 t
ORIGIN      
        1 ctattagggc aacatatcca aatatttcaa ccctgaccct gtatttaaca aaagggtttt
       61 ttcagatgga tctatggaac cggtttttat caattttctt gctccgcatg cagtggcagc
      121 gccctccggg caaacaaaga aaccttcgag tttaccgata agatcggtca tatcggtcat
      181 ctcttcatct gacaccgtaa tggccgaccc tccactatcc ctgattgcat ttaagatgag
      241 gtaatctcct atagcacttg gcaccctcat accagcggct agtgtagtag cattttccca
      301 aggctgtgcg aactcgtccc cattctgaaa cgccctgact attggagcac aattttcagc
      361 ctgcacagca aacatttttg gtcgttttga cgagatccat tccaaatctt ccatttcttg
      421 aaatgccttc cacattccaa caattcctgt tcctcctcct gttggataga taatgttatc
      481 tggcagttcc caatccaact gttcagcaat ttcatatccc attgttttct ttccttccac
      541 tctgtaaggc tcctgtaaag tggaaacatc aaacaggcca atttctgcag ccttttcccg
      601 cgaaatcgca ccggcatcgt ttatcaaccc atctatcaaa aataaattgc cgcctgaaac
      661 agatacttct ttttggtttg cttcgggagc atcttgcggc ataaacacaa aagcctccat
      721 ccctgcccga gcagcatatg aagccattgc accagcagca tttcctgctg atggcattgt
      781 taattttctt aaccccaatt cttttgcctt agaaacagca gccgacaatc ctcttgcttt
      841 aaatgaggct gttggattta aaccttcatc tttcaacatt agtgattcgg accccaattc
      901 ggaccccaat ctttttgttt caaaaatcgg agtgaaccct tctcctaaag tcacaatatt
      961 agactcgtca tacaccggca acatctcaaa atatctccac atattaggag ctcttgccga
     1021 aagcatatct ttagtaaaat acttggaagc ctccttcatg tcgtatcttg gataaagtac
     1081 ttttccacac ttctcacaaa gtctcctagg ttggtcggct gagaatattt cattgcaata
     1141 tgtacattct aaatttttta ggtagcttcg catttttgat aacctcttgg aaacaattag
     1201 tgtctaattc acttaataac aagacagaag tatgatacaa cggtcaagga aactggtgaa
     1261 ctgttcaaaa gcatgaatga gataaaaaat ggctgattac gacgtaataa taattggggg
     1321 aggcgctgct ggatttagtg caggaatgta ctcatctaga ttaggcatga agacattgtt
     1381 ggttgaaagg ttgattcctg gtggtcaaat tattaatgtc gaaagaatag aagactaccc
     1441 tggattactc gagggaataa gcggagctga tttctccagt aaagttcaag aacaggctat
     1501 tgcttcaggg accgaatttg ccatgtcgga agtaactgga atcagaaaat ctggaaagga
     1561 atggattgtc gagacctatg aagggcagaa aacctcaaaa tcagtgataa tagcaggtgg
     1621 atcaacactg gataaactaa atgttcctgg tgaagatgaa cttgtcgggg ccggcgtctc
     1681 gtactgcgcc acatgtgacg gagcattttt cattgatcaa aatgtgtgtg tggtcggtgg
     1741 tggagattca gctctacagg aagccataac actgacaga
//
LOCUS       DASCNS010000073         3379 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000571662, whole genome shotgun sequence.
ACCESSION   DASCNS010000073 DASCNS010000000
VERSION     DASCNS010000073.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000571662"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="QF838_02850"
     CDS             complement(<1..455)
                     /locus_tag="QF838_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013703.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HJO59664.1"
                     /translation="MSTGSWSVQQFIDRMFLSWYSPDALAAALPTGMLCFTLISLFMG
                     ASGYVSTFVAQYIGASRPERVGPAMWQGIYFSVLGGLLIAFTALFPKTIFSLIGHSPE
                     VQILESEYWVYLTLGGIFPVAQGAVAGFFSGRGKTYPIMWSTFAATAVN"
     gene            739..1752
                     /locus_tag="QF838_02855"
     CDS             739..1752
                     /locus_tag="QF838_02855"
                     /inference="COORDINATES: protein motif:HMM:NF021778.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HJO59665.1"
                     /translation="MRQILYVSLAGENRITCWSIDGDSGRLKHLKDTEVGDRPAPLAA
                     DLDNSLLFVGRRDLPCVTSYRFNSLTGDLIHVDDGPKLQGDPCYISLDKTNKYVLAAY
                     YEAGAVSVHSNNEGIFTGEKLWMQTGNGAHCIFTDPDNSHALLPHIAGERGINTILKY
                     DFNEDTGMLSPSETESVKQPDNRGPRHYTFHPSGEYVFFSNEQDSSVTSYRYQQGDME
                     ELHTISTLPAHYAGVNTCAQIKITPNGKFIYAPNRGHDSIAGFSVNSNTGQLKSIGRT
                     QTEAIPRVLDIDGTGQFLYSAGLGTGFISVFRISDNGNLEFIDRFQVGKEPMWILVMP
                     VFE"
     gene            1755..2237
                     /locus_tag="QF838_02860"
     CDS             1755..2237
                     /locus_tag="QF838_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018147454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HJO59666.1"
                     /translation="MENHATKITFKVNGEQHTLDIDNRFTLLDILRDRLNLTGAKKGC
                     DLGECGACTVILDGKAVNSCQILGVKITSSEIKTIEGLSQGDMLHPLQISFIENDGGQ
                     CGYCTPGFIMGAYPLFESKTNVSVEEIRLALAGNLCRCNAYGAIIDSVVGAIGIKVDG
                     "
     gene            2230..>3379
                     /locus_tag="QF838_02865"
     CDS             2230..>3379
                     /locus_tag="QF838_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HJO59667.1"
                     /translation="MVDQNIHSSSVGTSFQRTDLKDKVLGIAKYTADLSLPNMLHARV
                     LRSPHPHANILSIDVQPALKMAGVHDILTPFNVGVGKIAPDVPILDTKVRFVGDEVAA
                     IAAVNLYEADTALDLIKVSYDVLPFSTNIDTSLEMGNPKIHAQGNIVNGGPIVEARGN
                     VEEGFGQADYIVEDSFTTPDHHPASLEPRAALASWDGTRLTMWKPSRGIHADKAILAA
                     ALDVNPNNISVIGPVMGAGYGSKDETRTSILAALLSMRTGRPVKLELNREEEFLAGRR
                     HSTSTKIKMGVKKDGTVTAVHVKTVMDTGAYLSSGPGVVRRAGQGALYLYRCPNVKYE
                     GQIVYTNTPTAGSYRALGAPQGHFALESLSDQIAEKMNMDPLKFRLMNH"
BASE COUNT         1005 a          650 c          786 g          938 t
ORIGIN      
        1 atatttacag ctgttgcggc gaaagtagac cacatgattg gatatgtttt gccacgacca
       61 ctgaaaaacc cagctactgc accttgggca actgggaata tcccacctaa agttagataa
      121 acccaatact ccgattctaa gatttgtacc tcggggctgt gaccaatcaa agagaaaatt
      181 gtctttggga acaatgcagt gaaagctatc agtaagcctc ccaaaacgga aaaatatatt
      241 ccctgccaca tcgctggtcc aaccctttct ggtcgtgaag ctccgatgta ttgtgccacg
      301 aaggtactaa cgtaaccaga agcccccata aatagactga tcaaagtgaa acacaacatt
      361 cctgtgggca acgctgctgc caatgcatcg ggtgaatacc aagataaaaa cattctgtct
      421 ataaattgct gaacagacca ggatcccgta ctcaatatta gagggaacgc aacctttaga
      481 acttgcaggt aaccatttct ttgtcgccat cgttgggaaa aagacgattt tatgttgtag
      541 aggggcagat taatctcctg cataaaaatg aaacttatta aaggatggcc ttatcaattt
      601 tatcttctaa gttgacggaa tgtgaactat ttaattttat ttgagccaga cataataaaa
      661 agctaaataa aagcgttaac ttggaggtgc tgataaactt gtgattagct gattaataca
      721 gcatttcttg gaggcacaat gaggcaaatt ttgtatgttt cccttgcggg agaaaatcga
      781 atcacttgtt ggtcgattga tggtgacagc ggacgcctaa agcacttaaa ggatactgaa
      841 gtgggtgata gacccgcccc gttggctgca gacctcgata actcgcttct tttcgtaggt
      901 agaagagatc taccttgtgt cacatcttat agatttaata gtttgactgg agatctaatt
      961 catgtagatg atggtcccaa acttcaaggt gacccttgct acatttcatt ggataaaacg
     1021 aacaagtatg ttttggctgc atactatgaa gctggtgcag tttcggtgca ttccaacaac
     1081 gagggtattt tcactgggga aaagttatgg atgcagactg ggaacggagc ccattgtatt
     1141 ttcacagacc ctgataattc tcatgcttta cttccgcata ttgcgggaga aagaggtata
     1201 aacacgattt tgaaatatga tttcaatgaa gataccggaa tgcttagccc atcggaaaca
     1261 gaatctgtta aacaaccgga taacagagga ccgaggcatt atactttcca tcctagcggg
     1321 gagtatgttt ttttctccaa tgaacaagat agcagtgtaa cttcttacag gtatcagcaa
     1381 ggggacatgg aggagttgca cactatctct actttgcctg cccattatgc tggtgtgaat
     1441 acttgtgccc aaatcaaaat cactcctaat ggcaaattta tctatgctcc taatagaggc
     1501 catgattcaa tagctggatt ttcggttaac tcaaatacag ggcagctgaa atccattgga
     1561 aggactcaaa ctgaggctat tcctagggta ttggatatag acgggaccgg ccaattccta
     1621 tactccgctg ggcttggaac agggttcatc tcagttttca ggataagtga taacggtaac
     1681 cttgaattca ttgataggtt tcaggtaggt aaagaaccta tgtggattct tgttatgccg
     1741 gtgtttgaat aaatatggaa aatcatgcca cgaagataac ctttaaagtc aatggagaac
     1801 agcacaccct agatatcgat aacaggttca ctttattgga tattcttcga gacagattga
     1861 atctaacggg tgcaaaaaaa gggtgtgatt taggtgagtg tggtgcttgc acagtcatac
     1921 ttgatggtaa agccgtaaat tcatgccaaa ttctaggtgt gaaaataacc tcttctgaga
     1981 tcaaaactat tgaaggattg agccaggggg atatgttgca tccattacaa atttctttca
     2041 ttgagaatga tggaggccag tgtggctact gtacccctgg gttcataatg ggtgcatatc
     2101 cactttttga atcgaagaca aatgtttctg tggaagaaat aaggctggca ttggcgggaa
     2161 atttatgtcg ttgcaatgcc tatggggcca ttatagattc agttgtgggg gccataggaa
     2221 ttaaggttga tggttgatca aaatatccac tcttcatccg taggcacttc ttttcagcgg
     2281 acagatttga aagataaagt attgggtata gctaaatata ctgcagattt atctttacca
     2341 aatatgcttc acgcacgggt tcttagaagc cctcaccccc atgccaatat tttgagtatc
     2401 gatgtgcagc cggctttaaa gatggcgggg gttcacgata ttttgacccc ctttaatgta
     2461 ggtgtgggca agatagctcc ggatgtgccg attttggata ccaaagtgag atttgtcgga
     2521 gacgaggttg cggcgattgc ggctgttaac ctatatgagg cagatactgc cctcgattta
     2581 atcaaagttt catatgatgt attaccattt tcaacaaata ttgatacttc tttggaaatg
     2641 ggaaacccaa aaatacatgc tcagggaaat atagtaaatg ggggacccat agttgaggcg
     2701 agagggaatg ttgaagaggg ttttggtcaa gcagattaca tcgtagaaga ttcattcact
     2761 accccagacc atcatccagc ttccttggaa ccacgtgctg cgctcgcatc atgggatgga
     2821 actagactta caatgtggaa acccagcaga gggattcatg cagataaggc tattttggct
     2881 gctgctttgg acgttaaccc taataatatt agcgttatag gacctgtaat gggggcagga
     2941 tatggtagta aagacgaaac tcgaacttca attctagccg ccttgttatc tatgaggaca
     3001 ggtaggccag tcaaactgga actaaataga gaagaagaat ttttggctgg aagaaggcat
     3061 tctacttcta caaaaataaa aatgggtgtc aaaaaagacg gaactgttac tgcagtgcat
     3121 gtcaaaacag ttatggacac tggggcgtat ctttcgtcag gccccggtgt ggttagaagg
     3181 gcgggtcagg gggctcttta cctctacagg tgtcccaatg tgaaatatga aggccagatc
     3241 gtctatacaa atacccctac cgcagggtct tatcgggcat taggagcccc tcaaggtcat
     3301 tttgcattgg agtctttgtc cgatcagatt gctgaaaaaa tgaacatgga tcctctgaaa
     3361 tttcgactca tgaatcatg
//
LOCUS       DASCNS010000074         2660 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000595835, whole genome shotgun sequence.
ACCESSION   DASCNS010000074 DASCNS010000000
VERSION     DASCNS010000074.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000595835"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="QF838_02870"
     CDS             complement(<1..1077)
                     /locus_tag="QF838_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HJO59668.1"
                     /translation="MLSSKSNSTQRIEALGLVLVIFFAVCLRFYKLDAGYSGLGIYSA
                     TSLNVGTSLHNWFFPSIFIDGSIIADKPPLFFWIQGLFLKILGPTNLALRLPAALCGT
                     SCVLLLYLILKRTYGVLTAFIGGLLLAVAPIDVNFSRGVFLEPLTSALILISIFMLLI
                     GVQRKQVKFFYIAAAVIGLAFMSKLWQGLLPVPALAVFVVILRWEKWGVFLKTAVVSF
                     AIFIVTALAWPTLVWFFDSSYNTVMHSENIWDMIFGWNLFDRFGGLQYGSTHDSSWFW
                     FLSGPMQLFLGVTFIPLAVVGVWKTLNDLPGKVFSKTINDEKLVPCVGLIWLIWLLIC
                     VIGFGGATVRLSSYWASATPAICAL"
     gene            1218..2393
                     /locus_tag="QF838_02875"
     CDS             1218..2393
                     /locus_tag="QF838_02875"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59669.1"
                     /translation="MDKSKQPLPLAGLKVIEVAQGVAGPHCGRMLGAFGADVIKIEPP
                     SGDWSRHIGPFLGEDQNIESSAIYQYNNSGKKSVVLDLLVSKNISTLTDIVSEADVLI
                     EDWDIPTRLLNGIEKDRFTKSDSSLIEISLTPFGLSGPYSHFTSTPIIQLALGGYLYL
                     TGLPGKEPLMLPGHQPDYLTGLNGNGAVQIALWERDQTGAGQFVEVAMMETLTTLHQF
                     TMEMETYEGFIRNRNGNLWQRESGFANYGITTIECRDGHVCFGISTEDQWERLCLMIG
                     KGEFLKDSKYATRSARRSQATYLDSLLKEWVGERTRNDVMVEASEIWGLPTAPVLGIS
                     EVLEDEQFLHRGLFLEINHPITNKAYFPTFPFKTSGMVPDLSRSPLLGEHTSEVVGW"
     gene            2387..>2660
                     /locus_tag="QF838_02880"
     CDS             2387..>2660
                     /locus_tag="QF838_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59670.1"
                     /translation="MVSTKTINTLGSLTGLRVLDLTRVWAGPLATRIYGDFGAEIIKI
                     SDPRVPLVSPNGLNSKLNRNKKNIGLRLDKPEGRNIFLELTAISDVI"
BASE COUNT          866 a          566 c          570 g          658 t
ORIGIN      
        1 cagggcacaa attgcaggag ttgcagaagc ccaataactt gataaccgca cagtggcccc
       61 cccaaacccg attacgcaga ttaacaacca aatgagccat attagaccaa cacatggaac
      121 taatttttca tcattgatag tttttgaaaa tactttgcct ggtaaatcat tcaaagtctt
      181 ccatacaccg actacagcaa gagggataaa tgtaacacct aagaaaagtt gcatcggccc
      241 actcaaaaac caaaaccagc tagaatcatg agtggaaccg tattgtagcc caccaaatcg
      301 atcaaataga ttccagccaa aaatcatgtc ccatatattt tctgaatgca taacagtgtt
      361 ataagaagaa tcaaaaaacc aaaccagtgt tggccaagct agggctgtaa caatgaaaat
      421 agcgaaggaa actactgctg tctttagaaa taccccccat ttttcccaac gtaaaataac
      481 aacaaaaaca gcaagggctg ggacaggaag taagccctgc caaagttttg acatgaaagc
      541 aagcccaatg accgctgcgg ctatatagaa gaatttgacc tgcttccttt gtaccccgat
      601 aagtagcata aatatggaaa ttaatatcaa tgcggaggtc agaggttcca aaaaaactcc
      661 tctcgagaaa ttgacatcta tcggtgccac tgcaagaagt aatccgccga taaaggcagt
      721 taaaacccca taggttcttt tgagaatcaa atacagcagg agtacacaag atgttccgca
      781 taaagcggct ggtaaccgaa gtgccaaatt cgtgggtcca agtattttta aaaatagacc
      841 ctgtatccag aaaaaaagag gtggtttgtc ggcaataata gaaccatcta taaaaatgct
      901 ggggaaaaac caattgtgaa gggaggtacc aacatttaaa gatgttgctg aataaattcc
      961 taatccagaa tacccggcat caagtttgta aaagcgcagg cacactgcaa aaaaaatcac
     1021 taacactaag cccagggctt caatccgttg agttgagttg cttttagaag agagcataaa
     1081 ggataaagta ctattttatt tggggtaata caactgtgat tcaatgggac aagaatcaag
     1141 caaattccat aagatttatg taatatctcc agcaatgtgt ctaataaaca aactaactct
     1201 aatttcaaag tcgtccgttg gataaatcaa agcaaccctt gccactagca gggctgaagg
     1261 taatcgaggt tgcccagggc gtagctggcc ctcattgtgg tcgtatgctg ggggcttttg
     1321 gtgccgatgt catcaagata gaacctccct caggagactg gtctaggcat attgggcctt
     1381 ttctggggga ggatcaaaac atcgagtcaa gtgcaatata ccaatacaac aattccggta
     1441 aaaaaagtgt tgtgctggat ttacttgtgt ccaaaaatat ttcaacactc acagatattg
     1501 tctcagaagc cgacgttctg attgaggact gggatatccc tacccggctt ctaaatggta
     1561 tagaaaaaga tagattcacc aaatccgatt cctccttgat tgagattagc cttaccccat
     1621 tcggactttc tggaccatat tcccatttca cctctacacc tataatccaa ttagccctcg
     1681 gtggttatct atacctgaca gggctcccag gcaaggaacc tttaatgctt cctggccatc
     1741 aacctgacta ccttacaggg ctaaacggaa atggcgctgt tcaaattgcc ttatgggaaa
     1801 gagaccaaac aggagccggt caatttgtcg aggtcgccat gatggagacc ttaaccaccc
     1861 ttcaccagtt cactatggaa atggaaactt atgaaggatt tataagaaat agaaacggaa
     1921 acctctggca aagggaaagt ggattcgcaa attacgggat cacaacaatt gaatgccgcg
     1981 atgggcacgt ttgttttggg atatcgactg aagaccaatg ggaaaggctg tgccttatga
     2041 tcggcaaagg tgagtttctt aaagattcca aatacgccac tcgctctgct agacgttcac
     2101 aagcaactta tttggactcg ttactgaagg agtgggttgg ggaaaggact aggaatgatg
     2161 tgatggtgga agcctctgaa atttggggat taccgacagc cccagtattg gggatatctg
     2221 aggttttaga agatgaacag tttctgcata gaggtctatt tttggagatc aaccacccaa
     2281 taacaaacaa ggcttatttt cctacctttc cctttaaaac gagtggtatg gtccccgatc
     2341 tatctcgttc ccctttgtta ggtgaacata cctcagaggt tgtaggatgg tgagcaccaa
     2401 aacaataaat acactaggga gtctaaccgg ccttcgggta ctagatttaa ccagggtttg
     2461 ggcagggcca ttggcaacac gaatatatgg cgattttggg gctgaaatta taaaaatatc
     2521 ggacccaaga gttcctttgg tcagccccaa cggccttaat tctaagctca acagaaacaa
     2581 aaaaaatatt ggcctgcgtc tcgataaacc ggagggaaga aatatttttt tggaactcac
     2641 ggcaatctca gatgtcatca
//
LOCUS       DASCNS010000075         1963 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000617380, whole genome shotgun sequence.
ACCESSION   DASCNS010000075 DASCNS010000000
VERSION     DASCNS010000075.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000617380"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="QF838_02885"
     CDS             <1..174
                     /locus_tag="QF838_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HJO59671.1"
                     /translation="LPTGISPDGQLVEIVEYKSHPFMAGTQFHPEFLSRPTRPHPLFR
                     EFVAAAGGFEPNS"
     gene            516..1787
                     /gene="rho"
                     /locus_tag="QF838_02890"
     CDS             516..1787
                     /gene="rho"
                     /locus_tag="QF838_02890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HJO59672.1"
                     /translation="MKMKLLELQEKNDEELLNFAVEEGVVEDGATPRRMDILRKMFKA
                     YSEKDETVDADGILSILNEGYGFLRQNSDQRGSGDVYVSQSQIRRFSLRPGDYVVGNV
                     RPPKDGERYFGLVKVGQVNHSDPDSSKKRVQFEKLTPIFPEELIVMETEPKQLSTRLI
                     DLMSPVGRGQRGLIVSPPKAGKTTLLKELANGITENAPDIHIMVALIGERPEEVTDMK
                     RSIKGEVYSSTFDEPVEDHCRVAELVLERSKRLVESGEDVVILLDSITRLTRAYNLSV
                     PSSGRTLSGGMDPIALYPPKKFFGAARNTEEGGSLTILAACLVDTGSRMDDVVYEEFK
                     GTGNMEVHLDRKMAERRTFPALDIYKSGTRREELLLSEETLKQVWLLRRMITMIESDS
                     SNSSGEAMEKVMDRMRKTKNNEEFLTGLGKE"
BASE COUNT          601 a          363 c          479 g          520 t
ORIGIN      
        1 ctcccaacag gaatttcacc agacgggcag ttggttgaga tcgtagaata caaatcccat
       61 ccgttcatgg ccggcacaca gtttcacccg gaatttctgt ctcgtccaac caggccccat
      121 cccctgttta gagaattcgt agcggctgca ggtgggttcg aacctaattc ctgataatcg
      181 gattgtgttt attctggacg ctgattgacg aaagtgatat tattcccaca gtgctgctgg
      241 atgcagcatc gattctgacg atgaaattcc caatttatta tacgaatttc atttctggtt
      301 atgatggcgt ggaattagaa aaacgcgcta acctacactt taaaactcta gtgctcgagg
      361 atcgattatt cggttggtcg tgccgagttt gctttaaaag tgataccaga gtttcatccc
      421 aactttaaca cgggtgtagg taatacattc ctacgttaat ctgaattgaa aaattgggat
      481 taaatcaaac atgtaagaga agtagaaatc gaggtatgaa gatgaagttg cttgaacttc
      541 aagaaaaaaa tgatgaggaa cttttaaatt ttgcagttga agagggagtc gtagaagacg
      601 gcgctactcc tcgaagaatg gacatcttaa ggaaaatgtt taaggcctat tctgaaaagg
      661 atgaaacggt tgatgctgac ggaatccttt ccattctcaa tgagggatat ggatttcttc
      721 gccaaaacag tgaccagaga ggatctggtg atgtttatgt atcacaatca caaataaggc
      781 gttttagttt gaggcctggg gattatgttg ttggcaatgt cagacctccg aaggatgggg
      841 aaagatactt tgggttggtc aaagtaggcc aggtaaacca ttctgaccct gattcttcga
      901 agaaaagagt tcaatttgaa aaattgaccc caattttccc tgaagaactc atagtgatgg
      961 aaacagagcc aaagcagttg tcaacaaggt tgattgactt gatgtccccg gtgggtagag
     1021 gtcagcgagg gttgatcgta tctcctccga aggcaggaaa gaccaccctg ttaaaagagc
     1081 tcgccaatgg tattacggaa aatgcacctg atattcacat catggttgct cttattggag
     1141 aaagaccgga ggaagtgacg gacatgaagc ggtctatcaa aggtgaagtc tattcgtcta
     1201 cttttgatga accagtagaa gaccactgca gggtagctga actggttctt gaacgctcaa
     1261 aaagattagt tgagtcaggc gaggacgtag ttattttgtt agatagcatt accagactta
     1321 ccagggccta caatttgtcg gtccccagca gcggccgaac cctttctggt ggcatggatc
     1381 ctattgcctt atatccacca aagaaatttt ttggcgctgc cagaaataca gaggaagggg
     1441 gaagcctgac tatactggct gcttgcctag tggataccgg tagtaggatg gacgatgtcg
     1501 tctacgaaga atttaaggga accggtaaca tggaagttca ccttgaccgt aagatggctg
     1561 aacgaagaac tttccccgct ttagatatat ataagagcgg caccagacgg gaagaattgc
     1621 ttttgagcga agagacttta aagcaggttt ggttactcag aagaatgata accatgattg
     1681 agtctgattc ttccaattca tctggggaag cgatggaaaa agtaatggac aggatgagga
     1741 aaactaagaa caatgaggaa ttcctgaccg gcctaggtaa ggaataatag aaatccatat
     1801 tttctataaa gttttagatt aaaaaaatat ttggataaat atggcggaaa tgatattgac
     1861 ggaaccaatc actctgctaa tattttcgtg tgatctggtt aacaagccca agcattgccc
     1921 ttagcaaatg ttaaaaagcc agtaaaccag agtagtaaaa ggg
//
LOCUS       DASCNS010000076        14636 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000618263, whole genome shotgun sequence.
ACCESSION   DASCNS010000076 DASCNS010000000
VERSION     DASCNS010000076.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 14636)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14636)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14636
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000618263"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="QF838_02895"
     CDS             <1..1156
                     /locus_tag="QF838_02895"
                     /inference="COORDINATES: protein motif:HMM:NF023128.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59673.1"
                     /translation="LTSQSDHTDLILRLCIASVGLWWFGWSLWALKAIPEPVIFNKIE
                     GLTLSGAPRVALSELSKTFKELMQFRVILIYLAAYLLFNDGIQTVMGIATAFATDALK
                     IDQMFIILSLLIIQFVAAPSAMGFSKLASIWNTKKSLYIALAGWIVIVLFGVAIIPLE
                     PNKHADFDYQLIYNEAKSDYLLEKLPDLSDSKVDTAWRIEIGDLELNQSLSITATKSL
                     VEKIDTSDNSQYSISISGGNLDGITNIGRYHRSKLGDGALDWWPNLIRNSLWIPLGFT
                     AELQWIILGISVGLVMGGSQALARSLFAQITPETRSGEFFSFFGFMSRASSVFGPMLY
                     VVVTAMFDTRMAVASILSIIIAGTGILKWVDVEKGVIVAQEEDQKQRANS"
     gene            complement(1159..1923)
                     /locus_tag="QF838_02900"
     CDS             complement(1159..1923)
                     /locus_tag="QF838_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015000.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HJO59674.1"
                     /translation="MVHPLIIAHRGFSSERPENTISSFDHAFNNGFNLLELDVHLSSD
                     GVLVVMHDEKVDRTTNGTGFLKSMTYKEISQLDAGSWFRGTDEVSYTGQVVPSLDQFL
                     SRYSSKVHVFIEIKSLEEELIDKLKERLSAYGLIPKISSERLDIPGTSIISFNRAQIT
                     LSARAMPTVGHGLLVIKPTEDHIRFCVDNQIRGLFPNINFLSSELMDLAKSNSLHIGV
                     WGADSVEDLALVSNYEVLGVTVDWPSEAKKYFQGDI"
     gene            1994..2068
                     /locus_tag="QF838_02905"
     tRNA            1994..2068
                     /locus_tag="QF838_02905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2026..2028,aa:Thr,seq:cgt)
     gene            2237..4075
                     /gene="typA"
                     /locus_tag="QF838_02910"
     CDS             2237..4075
                     /gene="typA"
                     /locus_tag="QF838_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HJO59675.1"
                     /translation="MMIPPEKIRNITIIAHVDHGKTTLVDAFLKQNNIFTEREDPGEL
                     IMDSNPLEKEKGITILAKNTSIMYQGTKINIIDTPGHADFSGEVERVMNMADGCILLV
                     DSVDGPMPQTKYVLQQALTYGLTPIVVINKIDRPERRPQEVHAEVDDLFLELATKESQ
                     LEYPVLFASAREGYAIENMSDIPSDISPLIDVIVKEVPSPTGNPDDPLQILVTALDHD
                     DYAGQIAIGKISRGNVSSKSPAALISPDGEISNHIIETTFIYDGMKRSKTDNAGPGEI
                     VAITGLSEVNIGDTISDPSNPNPLPPIKVDEPTVKMTFGVNTSPFMGKEGMHHTSREL
                     LKRLKEELRTNVGLRVDQTENTDEFNVSGRGELHLSVLAETMRREGYEFQVSQAQPIL
                     KIIDGRLHEPFEYLHVETNEDYYGVLTENLNRRLGLLEDVVNDGNGTLRLTFLVPTRG
                     LIGFRSFFQNATHGDGIMSSRFAEYRRKEGKVRRSNQGFLVASEEGESVAYGLINSQE
                     RGKNLIDSGIQVYEGMIVGLNSRSSDLVVNVCKEKKLTNVRSSTSDIATKLIKPQTLS
                     LEESLDIISEDELIEITPASLRLRKRILSGEARLRQIKRAQKTGNG"
     gene            complement(4174..5598)
                     /locus_tag="QF838_02915"
     CDS             complement(4174..5598)
                     /locus_tag="QF838_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO59676.1"
                     /translation="MEENHLVLRGGRIIDPANNFDEVADIVIRCGKIQHISEIGVESS
                     GANTINLKGKWVIPGQIDTHAHVAGISRTVDPSLGYGMLAKAGTTTVVDMGGTGKNLI
                     DGIKRNGAGLNVAGQFALIPGLTIPSDNPSKGEIASTVSDALRQGCIGVKMLGGYHPF
                     SPETTSEIVRESNSQGAYVAFHVGSKDSGSHLGGLREIPEILGEGRLHICHVNSYCRG
                     VVKDSGDECKEAIAILSSLKGQINSEVYHAVANGTNGACDKDGNVIANVPRNCLVARG
                     FEPTIDGMRQAIMDGYASVISVKGGGISYIKGKEALDIYNKSKTMVGMSFPVNLPGSA
                     FNLTTEKDSVGDFVVDAVSTDGGSHPRNVAIQTSMALVRFGAFTPMDMASKLSWTPSR
                     MMGLLNKGHFSQGADADITVIDPHSGEPVMSFVDGDPIMINGEVIGKGGRILVTAAAE
                     RTMSDSGVKYQVIDLAESKLYQNY"
     gene            5684..6550
                     /locus_tag="QF838_02920"
     CDS             5684..6550
                     /locus_tag="QF838_02920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HJO59677.1"
                     /translation="MRLGAVFPQTESGTEVAAIKEYVQSVEAMGFDHILAFDHVLGAN
                     ASSRPGWSGAYQHTDSFYEPITFYSYVAGISTHIELATGVIILPQRQTALFAKQSATL
                     DLLSGGRLRLGIGTGWNQVEYEALGENFHNRGKRSEEQINLLRKLWAEELVTFKGDHH
                     IVTDAGLNPLPPRKSIPIWFGGMADPVLRRIAKFGDGWLPQGSPDDENKINFERLNRY
                     LEANNKSIEDIGIEGRISLNMTNSEIQKNFLAWGQLGATHVSVNTMNLGLQFPQEHLE
                     SLSNFIKIAKNS"
     gene            6581..7039
                     /locus_tag="QF838_02925"
     CDS             6581..7039
                     /locus_tag="QF838_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012787.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6713..6715,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HJO59678.1"
                     /translation="MLSVGDKAPDFTLAAHSGEPVTLSSFQGEKNVVLSFHIYSFTGG
                     UTHQVSSFRVHNAAFEEVDTQVLGISCDSKAAQSAFSGSLGGIPYPILSDFYPHGEVS
                     DSYGVFDEKRGTPKRAIVVVDKKGVIKFINTYRAAMDLNPIDVLETIKSL"
     gene            7011..7862
                     /locus_tag="QF838_02930"
     CDS             7011..7862
                     /locus_tag="QF838_02930"
                     /inference="COORDINATES: protein motif:HMM:NF020287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HJO59679.1"
                     /translation="MSSRLLNPFKISSLVMDSFNKQIFQAGDLAVLYDRRSREYMVQL
                     QESDQFESHIGNLNHTEIIGKPEGSWFPTTTGHLLLAFKPTRFQYTLNMPRVATIVYP
                     KDLGSITTYSNIFPGAKVVEAGSGSGSLTITLSELVGENGHVDSYDIRQDMSLSAKSN
                     LQKYNPGYNNVSFHIGDVSEAIPHHDVDSLILDLPEPWKVVNSAAQCLKRGGTFLSFL
                     PNISQVSDLVEKIKKHPEFSLINTIEIMERSWEISGRTMRPSHRMIAHTGFITTTRKC
                     IPRPKRT"
     gene            complement(7849..9024)
                     /locus_tag="QF838_02935"
     CDS             complement(7849..9024)
                     /locus_tag="QF838_02935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HJO59680.1"
                     /translation="MNSPARSLIGQTIMDDGPISFETFMEIALYSDVGYYQNEDIFGV
                     AGDYYTSPHIHPIFASLCALQVEKMWKTMDRPSPFFIIEQGAGDATLAKDFSEAISIL
                     NQDLYSSLTYTCVDRRQIEIADDSHMEIVQSDYVAPFHRTGVLISNELIDSFPVRMIE
                     VRDGEIFEIYVGIDTQGNFEEILIPITLDDYADFMPSNIRQLNGYRGPINTKMDDWYR
                     NISDALNCGFVITIDYGFDREVFFSMEKSHRLLQTYYKHIDGSNPFQRIGSQDITAHV
                     DFDTLKQSGYKYGFKEFGYFSQAEWLNSMGLDHLLRNMRKDSLEKSRIINLISGLNDI
                     SGLGGFKVLIQGKNVEHFDMRDLNANIFWPQELELPQVKNVHMANSKKLESRETKFF"
     gene            complement(9078..9269)
                     /locus_tag="QF838_02940"
     CDS             complement(9078..9269)
                     /locus_tag="QF838_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59681.1"
                     /translation="MSKKILVEECTHHWVIDQPNGPVSIGNCKLCGHKEEFRNSMPGS
                     GWDRSGQAKKNTTSESKSK"
     gene            complement(9314..10453)
                     /locus_tag="QF838_02945"
     CDS             complement(9314..10453)
                     /locus_tag="QF838_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HJO59682.1"
                     /translation="MTEENATLYRGLREVYIDRTTSSYIDGKAGKLYYRGFSIDDLAE
                     NCSFEEIIYLVMIGELPTSDQLSGFVAELKANMELPEAILDIIRITKDAHPMDVLRTA
                     ISALATFDPDVDDNSPEATLRKGTRLTAQAPTIAAAHSRIREGKDPVKPNPDLSIAAN
                     FLYMIFDEIPDPRDAKLIDKDFVLHAEHGLNASSFGARVAASTQADLHCAITTGVSVL
                     KGPSHGGAAESVMTMSLEIGSEDNAADYVRNTLDGGGRIMGFGHRVYTAVDPRSLHLQ
                     DDLKALGERKGEPKWYSILQKVVEVMQPYSRRGICQNVDFFSGAMYYLLGIPEDLFIS
                     IFAVGRIPGWTAQVVEQFENNILLRPRLQYVGELDREFIPIDNRS"
     gene            10807..11454
                     /locus_tag="QF838_02950"
     CDS             10807..11454
                     /locus_tag="QF838_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HJO59683.1"
                     /translation="MQNNNNLTQIEVPEVVVQRLPIYVRILSQLLDSKVEVISSHQLG
                     ELLQMTPAQIRKDLSYFGRFGKQGRGYNIQFLLDELRQILGIDKAWKACLVGLGRLGR
                     AIINYPGFSPEGFEIVAAFDNDIGQYNSSLGTITVQSMGEIARTVKAKDISIGIVAVP
                     SNQAQSVIDTLVENGISGILNYAPIAPFAPANVVMRNIDPVLSLQSMTFYMKDAE"
     gene            complement(11471..11713)
                     /gene="atpC"
                     /locus_tag="QF838_02955"
     CDS             complement(11471..11713)
                     /gene="atpC"
                     /locus_tag="QF838_02955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HJO59684.1"
                     /translation="MKLEVVTTEETVFSEDVDIVIAPGAEGQLGILPRHASLLTALNS
                     GDIVVRKDGQESVITVNGGFLEVAGDHVRILANSNQ"
     gene            complement(11731..13128)
                     /gene="atpD"
                     /locus_tag="QF838_02960"
     CDS             complement(11731..13128)
                     /gene="atpD"
                     /locus_tag="QF838_02960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HJO59685.1"
                     /translation="MATGKIVQVIGTVVDVEFPPDGMPLVYNALETTIGDGKLVLEVE
                     QHIGNNWVRCLALGPTEGLIRGTDAVDTGDAISVPVGDVTLGRLFNTLGETLDGLEEI
                     EGSEKWPIHRPAPSFEDQATEVEVLETGIKVLDLMTPFTKGGKIGAFGGAGVGKTVVI
                     QELINNIATEHQGVSVFAGVGERSREGNDLWYEMQEAGVLPQTVLVFGQMNEPPGIRA
                     RVAQTGLTMAEYFKEERGQDVLLFVDNIYRYILAGMEVSALLGRMPSAVGYQPTLGTE
                     MGALQERITSSKNGSITSVQAVYVPADDYTDPGIVTTFGHLDAVMTLERTLASQGLYP
                     AVDPLTSFSNILEPAIVGQEHYDVATGVRQVLQRYQELQDIIAILGIEELSDEDRATV
                     SRARKIQRFLTQPFSVAEVFTGQAGKYVALRDTVQGFKEILDGVHDELPEQAFYMVGT
                     IEEAVEKGAAMAEAS"
     gene            complement(13163..14020)
                     /gene="atpG"
                     /locus_tag="QF838_02965"
     CDS             complement(13163..14020)
                     /gene="atpG"
                     /locus_tag="QF838_02965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HJO59686.1"
                     /translation="MASVRQIARRIRSVENTAKITKAMAMIAASKLRRSQDAATQGRS
                     YAENMAAIVSNVVSQMNEDESHQPLLESRLIKNAGIAIIAPDRGLTGGLNANIFRSVS
                     DFIKSQSSPIKVVAIGKKSVDFTRRNDIETIAEYTGIGDRPGPGDTLPIAADIAEAFS
                     NEKVDSFHIAYGQFVNTTLQRPVIEPLLPVIPSEVVSNSVGYIFEPNAAEVLRRLLPR
                     YLETLVHHAILEGNASEQSARMVAMNQATDNANDMVGDLTLLMNKLRQETITTELLDI
                     VGGVAALES"
     gene            complement(14021..>14636)
                     /locus_tag="QF838_02970"
     CDS             complement(14021..>14636)
                     /locus_tag="QF838_02970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282001.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HJO59687.1"
                     /translation="AAKMDDAHGGGSITALPVIETQAGDVSAYVPTNVISITDGQIYL
                     EPELFNSGTRPAVNVGVSVSRVGSAAQTRAMRAVAGQLRLELAQYRELVTFAQFGTDD
                     LDASTRRQLERGQRSVEVLKQGQNVPLPVEQQVAILYALNEGYLDDVEITDIIRWEEA
                     FHAYMSSSHGDLLKVIVDEADLSDDSRTSLNSALEEFKKSGLAN"
BASE COUNT         4271 a         3315 c         2826 g         4224 t
ORIGIN      
        1 attgacttct caaagtgatc acactgattt gatcctgagg ctatgtatcg cttcggtcgg
       61 cttatggtgg tttggatggt ctctttgggc tttgaaagca atacctgaac ctgtaatttt
      121 taataaaatt gagggcctca ctttatctgg agcaccgaga gttgctcttt cagaattaag
      181 taaaacattc aaagaattaa tgcagttcag agtcatacta atttatctcg cagcatatct
      241 gctcttcaac gatggtattc aaacagtaat gggaattgct actgcctttg cgaccgatgc
      301 tttgaagatt gatcaaatgt tcataatatt atcccttcta ataattcagt tcgttgccgc
      361 accgtcagca atggggttca gcaaacttgc atcgatatgg aataccaaaa aatccttata
      421 tatagctctg gctggttgga tcgtaatagt tctatttggg gttgcaatta tcccattaga
      481 accaaataaa catgctgatt tcgactatca gctcatatat aacgaggcaa aatctgacta
      541 cttgcttgaa aaattgcccg atttatctga ctctaaagta gatactgctt ggaggattga
      601 aattggtgat ttggagctaa accaatcact ttctatcacc gccactaaaa gtttagttga
      661 aaagattgac acttctgata attctcagta cagcatttct atatctggtg gtaatctgga
      721 tggaattact aatatcggta ggtatcacag atctaaactg ggagatggtg ctttagattg
      781 gtggcctaat ctaataagaa actccctttg gatcccatta ggattcacag cagagcttca
      841 atggataata cttggcatct ccgtagggct tgtaatgggc ggaagtcagg ctttggccag
      901 aagtttgttt gcccaaatta cgcctgaaac ccgtagcggt gaattttttt ccttctttgg
      961 atttatgagc cgcgcgtctt cagtatttgg acctatgctt tatgtcgttg tcactgcaat
     1021 gtttgacacc aggatggcag ttgcatcaat tttatccata ataatcgctg gcacaggtat
     1081 tctgaaatgg gtagatgtag aaaaaggggt gatagtagcc caagaggaag atcaaaaaca
     1141 gcgagctaac tcatgaggct aaatgtcacc ttgaaaatat ttcttcgcct ctgaaggcca
     1201 atctacagta acccctaaca cttcgtaatt ggaaacaagg gctaaatctt ccactgaatc
     1261 ggcaccccac actcctatat gcaaagaatt ggatttagcc aggtccatca attctgagga
     1321 caaaaaattt atgttaggaa acaatcccct tatttgatta tccacacaaa accttatgtg
     1381 atcttcagta ggtttaatta ccaacaaacc gtgacccacg gtcggcatag cgcgggccga
     1441 caaagtaatc tgagctcgat tgaacgaaat tattgacgta cccggaatat caagtctttc
     1501 gcttgaaatt ttaggaatta aaccataggc actcaatcgc tccttgagtt tatcaatcaa
     1561 ctcttcttct aatgatttaa tctctataaa aacatgtacc ttactactat atctagatag
     1621 gaattggtct aaacttggga ctacttgacc agtgtaagaa acttcatctg tgcctcgaaa
     1681 ccaagagcca gcatcaagtt ggctgatttc tttatatgtc atcgatttca aaaagcctgt
     1741 tccatttgtg gtcctgtcca ccttttcatc gtgcatgacc accagcacac catcagagga
     1801 caggtgcaca tccagttcca acagattgaa cccgttgttg aacgcatgat caaaactact
     1861 gatcgtattt tcaggtcttt cagatgaaaa ccctcggtgg gcaataatca aaggatgaac
     1921 caaagtgtct ccccatcatt tctattaaat agaaacaaag catatagtaa aattgtatcc
     1981 gctccaatga tatgccaacg tagctcagtg gtagagcagc tctttcgtaa agagcaggtc
     2041 gtcggttcaa tcccgaccgt tggctccaca aaattatctt attcctcttt tgattggaaa
     2101 tccaaatata tattcaaaac gcagctcgac cccgatttag ataaattaac ccttaagccg
     2161 tttgatatcc cctctgcatt taatgattga tatgagatca ttgatgtgta aatagttttg
     2221 taaaggacct ctgtcgatga tgatcccacc cgaaaaaatt agaaatatca ccataattgc
     2281 tcatgttgat catggtaaaa ctacacttgt agatgctttc ctgaagcaaa acaacatttt
     2341 cactgagcgt gaagaccctg gcgaattaat aatggacagc aatcctcttg aaaaagaaaa
     2401 gggaattacc atattagcca aaaatacttc aatcatgtat cagggcacca aaataaatat
     2461 catagatacc cctggccacg ctgatttcag cggagaagta gaaagagtaa tgaatatggc
     2521 cgacgggtgt attctactcg tggattctgt ggacggacca atgccacaga ccaaatacgt
     2581 tctccagcaa gcattgacct atggactgac cccaatagtg gttatcaaca aaatagatcg
     2641 gcccgaacga aggccacaag aagtgcacgc tgaagttgat gatttatttt tagaattggc
     2701 gacaaaggaa tcacagttgg aatatccagt tctttttgct tcagcccgtg agggatatgc
     2761 tatcgaaaat atgtctgata tccccagtga cattagtcct ttgatagatg tgatagttaa
     2821 ggaggtacca tcaccaaccg gaaatccgga tgaccctttg caaatattag ttacggcatt
     2881 ggaccatgat gactacgcag gccaaatagc gataggaaaa atatcacgtg gaaatgtatc
     2941 cagcaaatca cctgctgctt tgatttcacc tgatggggaa atttctaatc acataattga
     3001 aactaccttt atatatgacg ggatgaaaag aagtaaaacc gataatgcag gccctggcga
     3061 aattgtcgct attactggct tatctgaggt aaatattgga gatacaattt ctgatcccag
     3121 caaccccaat ccactacctc ctatcaaagt tgacgaaccc acagtaaaaa tgacctttgg
     3181 tgtcaatact tcgcctttca tgggaaagga aggaatgcac cacacctcaa gggagctctt
     3241 aaaaagactc aaggaagagc tacgtaccaa tgtggggcta cgggtagatc aaactgaaaa
     3301 tactgacgag tttaatgtca gcggtagagg cgagttacat ttgtcagttc ttgcagaaac
     3361 catgcgaaga gaaggctatg aatttcaagt atcgcaagcg caaccgatat tgaaaataat
     3421 tgacggccgc cttcacgaac ctttcgaata tttacatgtt gaaacgaacg aagattacta
     3481 tggggtcttg actgagaatt tgaaccgtag actagggtta ttagaagacg ttgtgaacga
     3541 cgggaatgga acccttagat taacattttt ggtcccgaca agaggcctta ttgggtttag
     3601 gtcttttttc caaaatgcga cacatgggga tggcattatg tctagtaggt tcgctgaata
     3661 cagaagaaaa gaagggaaag ttagaaggtc gaatcaagga ttccttgtag cttcagaaga
     3721 aggagaatca gtggcatacg ggcttattaa ctctcaagaa cgtgggaaaa atctcataga
     3781 tagcggaatt caagtttatg aaggtatgat agtcggccta aactcaagat cttcagattt
     3841 agttgtaaat gtgtgcaaag agaagaaact aactaatgtg cgctcttcaa catctgatat
     3901 agccaccaag ctaataaagc cacaaacact ttctttagaa gaatctttgg acattatttc
     3961 tgaagatgag ctaatagaaa taactcctgc aagccttaga ctacgaaaaa gaatactttc
     4021 aggggaagcc aggcttcgcc agataaagcg agcccaaaaa accggtaatg gctaattgaa
     4081 taaaacccca tctttatttg aataacagtc aaaagaccaa gttaagatta gttatacgcc
     4141 caaccgtaat aacgggaggt taaataacca tctttagtaa ttttgatata acttactttc
     4201 ggccaaatca ataacttggt atttcacccc actgtcagac atcgtacgct ctgccgcggc
     4261 agtgaccaaa atccgacctc ctttgccgat cacctcaccg ttgatcatta ttggatcccc
     4321 atccacgaaa gacattaccg gttctcccga atgggggtct atcacggtaa tatctgcatc
     4381 agccccttga gaaaaatgcc ccttgttcaa gaggcccatc attctagaag gtgtccaaga
     4441 taacttactg gccatgtcca taggagtaaa tgcgccaaat cgaaccaagg ccatgctagt
     4501 ttggatagcc acatttcgag gatgggaacc tccatccgtg ctgacagcat cgacgacaaa
     4561 atcgccaaca ctatcctttt ctgtagtcaa attaaaagcc gagccgggta ggttaacagg
     4621 aaaactcatc cccaccatag tcttcgattt gttatatata tctaaagcct ctttgccttt
     4681 tatatacgat atcccccccc ccttaacact gataacagat gcatacccat ccattattgc
     4741 ttgcctcata ccatcgatag tcggctcaaa gcctcttgcc accaaacagt ttctaggtac
     4801 gtttgcaatt acattcccgt ctttgtcgca ggccccattc gtaccgtttg ccacagcatg
     4861 ataaacctca gaatttatct gacccttcaa ggaactaagg atagcaattg cctccttgca
     4921 ctcatcccct gaatctttca caacccccct gcaataagaa ttgacatgac atatatgtag
     4981 ccttccttcg cctaaaatct ctggtatctc ccgcaaaccg ccaagatgac tacctgaatc
     5041 cttggaaccg acgtgaaacg ccacatatgc cccctgggaa ttcgattctc taacaatttc
     5101 cgatgtggtt tctggggaga aaggatggta tccacctaac atttttacac ctatacaacc
     5161 ttgcctcagg gcatcggaaa cagtactggc tatttctccc ttggaagggt tatcagaagg
     5221 aatcgtaagt cctgggatca aagcaaattg gccagccaca ttcagcccgg ccccatttcg
     5281 tttaataccg tcgattaaat ttttccccgt accacccata tccacaacag tcgtggtgcc
     5341 ggcttttgcc aacattccat accccaaaga aggatccact gttctggaaa taccagcgac
     5401 atgagcatga gtatcaattt ggccaggtat gacccatttt ccctttaggt ttatggtgtt
     5461 tgcgcctgaa ctctcaactc caatttccga tatgtgctgt atttttccac acctaatgac
     5521 aatgtcagct acctcatcaa agttattggc aggatctatt atcctgccac ccctcaacac
     5581 caaatggttt tcttccataa attcacgaat cctgcgaagg caatatatat tgcacatata
     5641 gtaaactagc aattgaaaat ccaacaataa taggaatatt acgatgcgtc ttggagccgt
     5701 ttttccccaa actgagtctg gtacagaggt agcagcgata aaagaatatg ttcaaagtgt
     5761 agaagccatg gggtttgatc acatcttggc ttttgaccat gtactgggtg caaacgcctc
     5821 aagccgccct ggatggtctg gtgcttatca acatactgat tcattttatg aaccgattac
     5881 tttttatagc tatgtggcag gaatttcaac ccacatagaa ctcgccaccg gggtaatcat
     5941 tctgccgcaa aggcaaacag ccctttttgc caagcaatca gccacactcg atctcttgtc
     6001 aggtggtcga ttacgactag gaatcgggac aggttggaat caagttgaat atgaagcgct
     6061 tggagagaat ttccataatc gtggtaaacg atcagaagaa caaataaacc tgctaaggaa
     6121 attatgggca gaagaattag tcacatttaa gggagatcac catattgtca cagatgccgg
     6181 tttaaacccc ttacctccaa gaaaatcgat ccctatttgg tttggaggaa tggcagaccc
     6241 tgttttacgt cggatagcta aatttggaga cggatggctt cctcaaggtt caccagacga
     6301 tgaaaacaaa atcaattttg aacgtcttaa ccgctattta gaggccaata ataaatcgat
     6361 agaagacata ggaatagagg gtcgcatttc ccttaacatg acgaattctg aaatccagaa
     6421 aaatttctta gcatgggggc aactaggtgc cactcatgtc tctgtaaata ctatgaacct
     6481 tgggctgcaa ttcccacaag aacatcttga gtcactttcc aattttatta aaatagctaa
     6541 gaattcttag ctagaaaagc tcgatgataa ggagaaattc atgttatctg taggcgataa
     6601 agccccagac ttcacccttg ctgcccattc tggggagccg gtgacattaa gctcatttca
     6661 gggagaaaag aatgtagttc tttcttttca tatatattct tttacgggtg gttgaaccca
     6721 ccaagtttct tctttcagag tgcacaatgc tgcatttgaa gaagtagata cccaagttct
     6781 gggtattagt tgcgattcta aggctgccca atccgcattt tcaggatcct tgggtggtat
     6841 accctaccca atcttgtcag atttttaccc tcatggggaa gtatcagatt cttacggggt
     6901 gtttgatgaa aaaaggggta caccaaaaag ggctatagta gtggttgata agaaaggtgt
     6961 cattaaattt atcaatacct atcgtgcggc tatggattta aatcctattg atgtcctcga
     7021 gactattaaa tccctttaaa atctcatcac tggttatgga cagttttaat aagcaaatat
     7081 ttcaagccgg agacttggct gtcttatatg acagacgttc tagggagtat atggtccaat
     7141 tacaagaatc ggaccaattt gagtctcata ttggaaactt aaaccatacg gagataatag
     7201 gtaaaccaga aggaagttgg ttccctacaa ccaccggcca tttactgcta gcttttaaac
     7261 caacaagatt tcaatacacc ttgaatatgc cacgagtggc cacgattgta tatcctaaag
     7321 acctgggatc tataactacg tattccaata ttttccccgg cgccaaagta gtcgaagccg
     7381 gttccggttc tggttctctt acaattactt tatctgaact ggtcggagaa aatggacatg
     7441 tagatagtta cgacataaga caagatatgt ctttatcggc taaatctaat ctacaaaaat
     7501 acaatcctgg ctacaacaat gtgtcttttc atatcggaga cgtctctgag gcaatacctc
     7561 accatgatgt tgacagtcta atcttggacc tccctgagcc ttggaaggtc gttaattcag
     7621 ctgcacagtg cctgaaacga ggtggtactt tcctatcctt tctcccaaat atatctcagg
     7681 tttcagatct ggtggaaaaa ataaaaaaac acccagagtt ttccttgatt aacactattg
     7741 aaataatgga aagatcctgg gaaatttctg gacgaacaat gaggccctct catcggatga
     7801 ttgctcatac cggtttcata accaccacac gaaaatgtat tccaaggcct aaaagaactt
     7861 agtttccctg ctttccaatt ttttagaatt tgccatatga acatttttga cctgaggcaa
     7921 ttctaactct tggggccaaa aaatattcgc atttaaatct ctcatatcaa aatgttccac
     7981 atttttgcct tgtatcaaaa ccttaaatcc tcccaaccca gaaatatcat tcagtcctga
     8041 tattaaatta atgatcctac ttttttctag gctgtctttt ctcatgttac gaagcaaatg
     8101 atctaaaccc atcgagttta gccattctgc ctgtgaaaaa tatccgaatt ctttaaatcc
     8161 atatttatat ccactttgtt taagagtatc aaaatccacg tgagctgtta tatcttgaga
     8221 tccaattctc tggaacgggt tggagccatc tatatgctta taataggttt gaagtagtct
     8281 gtgtgatttt tccattgaaa agaaaacttc tctatcaaaa ccataatcaa tggttattac
     8341 aaatccacag tttaaagcgt cggaaatatt tctataccaa tcatccatct tggtatttat
     8401 ggggcccctg tacccattca actgtctgat attagaaggc ataaaatcgg catagtcatc
     8461 gagtgtgata ggaattaaaa tctcttcgaa attaccctga gtatctatac caacatatat
     8521 ctcgaaaatt tctccatctc tcacttcgat cattcttact ggaaaggagt caatcaactc
     8581 atttgatata agtactccgg ttctatgaaa aggtgcaaca taatcagact gcacaatttc
     8641 catatgactg tcgtcagcaa tctctatctg tcgcctgtcc acgcatgtgt aagtaaggga
     8701 ggaatacaaa tcttgattta gaatcgaaat agcttctgaa aaatcctttg ccaaagtggc
     8761 atctcctgct ccctgttcaa taataaaaaa cggggaaggg cgatccattg ttttccacat
     8821 tttttcaact tgaagagcac acagcgaggc aaatattggg tgtatatgcg ggctggtgta
     8881 gtaatctccc gcgaccccaa aaatatcttc attttgataa taaccgacat ctgaataaag
     8941 ggcaatttcc ataaaagtct caaaggaaat tgggccatca tccataatag tctgcccaat
     9001 taaacttcga gctggagaat tcaattaatc aactccagag tatataactc aaagatttat
     9061 tattttaaat taatgaccta tttcgacttt gattctgatg ttgtgttttt tttggcctgc
     9121 ccgcttctgt cccagccgga gccaggcatc gaatttcgga actcttcctt atgcccacag
     9181 agcttacagt taccaatgct caccgggcca ttgggttgat ctataaccca gtgatgggta
     9241 cattcttcca ccaatatttt cttactcaaa gatactcctt tctagaggta taacagttgg
     9301 tttaagggaa aaactatgaa cgattatcta tcggaataaa ttcccggtct agttccccta
     9361 cgtactggag tctaggtctc agtaaaatat tattttcaaa ctgctcaact acctgagccg
     9421 tccatccagg tatacggcca acggcgaata ttgatatgaa taaatcttca gggatcccta
     9481 ataagtagta catggcacct gaaaagaagt ctacgttttg gcaaattccc cgtcgggagt
     9541 aaggctgcat aacttccaca accttttgga gtatggagta ccattttggc tctccctttc
     9601 tttccccaag agcctttaag tcgtcttgca gatgcaaaga tcgaggatca acggccgtat
     9661 aaactctgtg gccaaatccc attatccgtc caccaccatc tagggtattt cttacatagt
     9721 cagcagcatt atcttcactt cctatttcta acgacatagt cataaccgac tctgctgctc
     9781 caccgtgtga aggacctttc aaaaccgata cgcctgttgt aatggcgcaa tgcaagtcag
     9841 cttgagtaga tgccgcaact ctagccccaa atgaagaagc atttaatccg tgttccgcat
     9901 gaagcacaaa atctttatca atgagtttgg catctcgggg atcaggtatc tcatcaaata
     9961 tcatatatag aaaattagca gcaatgctaa ggtccgggtt gggtttaact ggatcttttc
    10021 cttctcttat acgtgagtga gcagcagcta tggttggggc ctgtgcggtg agtcttgtgc
    10081 ccttcctcaa tgtggcttcc ggcgagttgt catctacatc tggatcaaat gtagccaatg
    10141 cagaaattgc tgttctcaat acatccatcg gatgtgcgtc cttggtgatc cggatgatat
    10201 ctaaaatggc ctctggaagc tccatattgg ctttaagctc cgcaacaaac ccagatagtt
    10261 gatcacttgt gggaagctca ccaatcatca ccaaatatat gatttcttcg aaactacaat
    10321 tttctgctaa atcatcaatt gaaaacccac ggtagtatag tttgcctgct ttgccgtcaa
    10381 tgtagctcga tgtagtccta tctatgtaaa cctctcgcaa acccctgtat agagtcgcat
    10441 tttcttccgt catcgattac ctccaagtga ctaatcaaac aagctttata tcaccctaaa
    10501 ccaattagat tgcatccagg tgaaacaatt tagcagtttt ttaatagaaa aaagccttgc
    10561 aattttatca atcgaggcaa attttagtca agaagggaaa aagggccagc aaaattgaaa
    10621 caacttatca tattaattgc cttgtaatag tcgtcaggca atttttaacc aatctgctca
    10681 agaaagttac ttctaattct tttcattgaa tatttatctc ttctccagtt aaatatttgt
    10741 taatttgcaa aaatgctcta caatcaagct gctccacttt tcttaatagc catataatgt
    10801 ccggtattgc aaaacaacaa taacctcacc caaattgaag tacctgaagt tgtagtccaa
    10861 agactgccca tatatgtgag aatcctcagt cagctactgg attctaaagt ggaagtaata
    10921 agctctcatc aactcgggga acttctacaa atgacaccag cgcaaatacg aaaagacctg
    10981 agttacttcg gaagatttgg aaaacagggt cgaggataca atattcaatt ccttcttgat
    11041 gagctcagac agatattggg aatcgataaa gcctggaagg cgtgtctggt aggtttagga
    11101 aggcttggta gagcgataat aaactatcca ggcttttctc ctgaaggatt tgagatagtt
    11161 gctgcctttg acaatgatat cggccaatac aactctagtt tggggactat tacggtccaa
    11221 tctatggggg agattgccag aacagtcaaa gccaaagaca tatcgattgg aatagtagct
    11281 gtaccttcga atcaagctca atccgtaata gacactctag tcgagaacgg cataagtggt
    11341 attttgaatt atgcgcctat agctccattt gcaccggcca atgtggtgat gcgaaatatt
    11401 gatccagttc tatctttgca atcgatgacc ttttatatga aggatgctga ataattaact
    11461 aaagcgaata ctattgattg ctattggcga gtattctgac atggtctccg gcgacctcta
    11521 gaaacccgcc attaactgtt ataactgatt cttgtccatc tttccgcact acaatatccc
    11581 ctgaattaag agccgtaagc aaagaagcgt gcctaggaag tattcctaac tggccttccg
    11641 cgcctggtgc gataactata tcaacgtcct cagaaaaaac ggtttcttca gttgttacaa
    11701 cttctaattt catagtattg cctttattgc ctatgaggcc tctgccattg ctgcgccttt
    11761 ctcaacagct tcttcaatcg ttcccaccat gtagaaagct tgttcaggca attcatcatg
    11821 tacaccatca agaatttctt tgaaaccttg caccgtgtcc cttaaagcaa cgtatttacc
    11881 tgcttgtcca gtgaaaactt ctgcaaccga gaaaggttgt gtcaggaacc tttgaatctt
    11941 tctagccctg gacaccgtag ctcgatcttc atccgatagt tcttcaattc ccagtatagc
    12001 gataatatcc tgcagctcct gatatcgttg gagaacctgg cggacaccag tggccacatc
    12061 ataatgttcc tgcccaacaa tggcaggttc caagatgttg gagaatgagg tcaacgggtc
    12121 cactgctgga tataatcctt gggaagcgag tgttctttct aaagtcatca ccgcgtccaa
    12181 atggccaaat gttgtgacga tacctggatc agtataatca tcggctggaa catatacagc
    12241 ttgcactgat gtaatggagc cattctttga ggaagtaatc cgttcttgta gcgcacccat
    12301 ctccgttcct aaggtaggct ggtatccaac agcagaaggc attcttccta aaagtgcaga
    12361 gacttccatc ccagccagga tatacctgta gatattatct acgaaaagaa gaacatcttg
    12421 ccctctctct tccttgaaat attctgccat tgtcagtcca gtctgtgcta cgcgggcccg
    12481 aatgcccggc ggctcgttca tttgtccgaa taccaacact gtctgaggca aaacccctgc
    12541 ttcttgcatc tcataccaaa gatcatttcc ttccctcgag cgttcaccaa cccctgcaaa
    12601 cacagacacc ccttgatgct cagttgcaat attgtttata agctcctgaa taacgacggt
    12661 cttccctact ccagctccgc caaacgctcc aatttttccg ccttttgtaa agggggtcat
    12721 caaatccaat acttttatcc cagtctctag tacttcgact tcagtagctt ggtcttcaaa
    12781 ggaaggagct ggcctatgaa tcggccattt ttctgatcct tcaatttctt ccaacccatc
    12841 aagagtttct ccaagagtat tgaatagtcg gccaagagta acatctccca ccggtactga
    12901 aattgcatct ccagtatcca cggcgtcagt gcctctaatt agaccttcag tcggacctaa
    12961 ggccaagcac cttacccagt tgttaccgat gtgttgttcc acctcaagga caagttttcc
    13021 atcccctata gtagtctcca atgcgttata caccagaggc ataccatcag gtgggaattc
    13081 cacatccacc actgttccaa ttacttgtac tatctttccg gttgccattt tatactcctg
    13141 agcctaaaca gcattaaatt ttttagcttt caagtgcggc tactccccct acaatatcga
    13201 ggagttctgt tgtgattgtc tcttgtctta atttattcat tagcagagtc aagtctccaa
    13261 ccatatcgtt ggcattatca gtagcctgat tcatagccac cattcttgcc gactgttcac
    13321 ttgcattccc ttctaatatc gcatgatgta ccaaagtctc aaggtacctt ggaagcaatc
    13381 ttctaagcac ttcagctgca tttggttcaa atatatagcc aacagaattg gatacaactt
    13441 cactaggtat tacgggaagt aacggctcta tgacaggtct ctgaagtgtt gtgttgacaa
    13501 actgaccgta ggctatatgg aatgaatcta ccttttcatt tgaaaatgct tcagctatat
    13561 ccgctgcaat cggtagagtg tcacccggac cgggtctatc accaatgccg gtatattcag
    13621 caattgtttc aatgtcattt cgtcttgtga aatcaacact ttttttacct attgctacta
    13681 ctttaatagg gctgctttgc gatttaatga aatcgctaac agacctaaat atgttggcgt
    13741 ttagcccacc ggtaagacct ctgtctggtg caattatggc aatcccggcg ttttttatca
    13801 ggcggctttc tagcaagggt tggtgggatt cgtcttcatt catctgggac accacattgg
    13861 aaactatcgc agccatattt tccgcgtacg acctaccttg agtggcagcg tcttgactcc
    13921 tcctcaattt cgaagccgcg atcatagcca tggctttggt aattttcgcc gtattttcga
    13981 cgcttctgat acgcctagct atttgtctga cactagccat ttagttagcc aacccacttt
    14041 ttttgaattc ttccaacgca gaattcagcg aagtccgaga gtcatcgctc agatctgcct
    14101 catctactat aactttcaaa aggtctccat ggctggaact catatatgca tggaatgctt
    14161 cttcccaacg gattatgtca gttatttcca cgtcgtctaa atatccttcg tttaaggcat
    14221 ataagatagc gacttgctgt tcaaccggta aaggcacatt ttgaccttgt ttcaaaacct
    14281 caacactcct ctgtcctctt tccaattgtc tacgtgtaga tgcgtcaaga tcgtcagtcc
    14341 caaattgagc aaaagttaca agttccctat attgtgctag ctcaagtctc agctgccctg
    14401 caactgccct cattgcccta gtttgggcgg cactccctac acgggagaca gaaactccca
    14461 cgttaacagc tggccgtgtc cccgagttga agagctccgg ttccaagtat atttgtccat
    14521 ctgtaatgga tataacatta gtggggacat aagctgatac gtctccagct tgagtttcaa
    14581 ttactggcaa agccgttatc gacccaccac catgagcatc atccattttt gcagcc
//
LOCUS       DASCNS010000077         3726 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000632195, whole genome shotgun sequence.
ACCESSION   DASCNS010000077 DASCNS010000000
VERSION     DASCNS010000077.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000632195"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="QF838_02975"
     CDS             <1..635
                     /locus_tag="QF838_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HJO59688.1"
                     /translation="PTLLQEKKFQFAHGDLVHALKEECSPTAVSITTVNTQEKYPGNT
                     VENVPELTQAQYKIETSVKLEVSSKQAFEFFSSPLNLGLATPDWMDFHITESSSDMNK
                     GSEFEYKINLGPFPIKWRTEIINWVPDNLFVDYQKKGPYSLWWHQHRIVTEGVSTCRM
                     EDKVFYRVPGWILGRIAHKYIIKNILVRIFAYRRKVIQMRFGGRIYDSSQ"
     gene            619..1533
                     /locus_tag="QF838_02980"
     CDS             619..1533
                     /locus_tag="QF838_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014375.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJO59689.1"
                     /translation="MTRRNNDDFVLLNRFKGRLLNHLTSVDRTGSVSVIEEALRYNCT
                     PASIYNSVIIPVMVDLGELWHGGRITIAHEHHASQIVDGLINMVVQKSQRLSINGLRA
                     LVTSPSGENHWIGAKIFANLLFLEGWDVEYIGVDIPAEDLAIYLEEVEVDVVAFSVVL
                     PGNIMSVEDAVSTIESLDNPPFVILGGPAFKGFSGEIGKAVIVDNFATGLTVLQDAMG
                     IGAGNLSLQDTLLGVGGKVLEIRRAKGMNQGELAVLAGVDRAYISLVENGKQNLTMAA
                     LFKLAEALGTSVNTLLAFPANRIFCPKD"
     gene            complement(1602..2177)
                     /locus_tag="QF838_02985"
     CDS             complement(1602..2177)
                     /locus_tag="QF838_02985"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HJO59690.1"
                     /translation="MNIIILSAIWAVASYFIGTVSVGDLTSKLKNVDIRSVGTGNPGA
                     SNIYAEVGPIYGVGVFFIDVAKGLIVTLPVIFFKYPTWIATIGIIFFLIGHMIIRPWG
                     KPGGIGMATTMGAGIVLLPVGALIAVLPSFLILIITRRPAYTGVFAFVSTIIAGWLVH
                     FDLIATTTVIILAGAVLVKFQFQYRELNPHQ"
     gene            3292..>3726
                     /locus_tag="QF838_02990"
     CDS             3292..>3726
                     /locus_tag="QF838_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59691.1"
                     /translation="MTLVILTLTAVFTACTTEVVKEVPVGVTIEKEIVKTLEAPVVEN
                     TVKDLDPGELILYSGRSESLVGNIVAQFEEMTGIDIQVKYGKTFPVATMILEEGSNSP
                     ADVYFAQDPGGLGFLAKEGRLIELPDTITDRVADWAKPNDGKW"
BASE COUNT         1155 a          679 c          799 g         1093 t
ORIGIN      
        1 acccaactct tttgcaggaa aaaaagtttc agtttgctca cggagattta gtacatgctt
       61 tgaaagagga atgttctccc acagcggtca gcatcaccac agtaaataca caggaaaaat
      121 accctgggaa tacagttgaa aatgtacctg aacttactca ggctcaatat aaaattgaga
      181 cctccgtaaa attagaggta agttcaaaac aggcttttga atttttttcc tctcctctta
      241 acttgggact ggccaccccg gattggatgg attttcatat aactgaatcc tcatccgata
      301 tgaataaagg ttcagaattc gaatacaaaa tcaatctggg tccctttcca attaaatgga
      361 ggacagaaat aattaattgg gtgccagata atttatttgt ggattaccaa aagaagggtc
      421 catacagcct gtggtggcac caacaccgta tagttacgga aggggtttct acttgtcgga
      481 tggaagacaa agttttttat agggtaccag gttggatttt gggtaggata gctcacaaat
      541 acataataaa aaacatatta gtaaggatct tcgcttacag aagaaaagtt atacaaatgc
      601 gtttcggggg taggatttat gactcgtcgc aataatgatg attttgtttt attgaaccgc
      661 tttaaagggc gattgctgaa ccatttaact tcagttgata ggactggaag tgtctcggtg
      721 attgaggagg cattgcgtta taactgtaca ccagcctcta tatataattc agttataatt
      781 cctgtaatgg tggatctagg agagttatgg catggaggac gaatcaccat cgctcatgaa
      841 catcatgcca gccaaattgt ggatggtctt ataaatatgg tggtccaaaa atcccaacga
      901 ctctcgatta acggtcttcg tgcgctggta acatctcctt caggtgaaaa ccactggata
      961 ggggcaaaaa ttttcgccaa tttgctattt cttgaaggtt gggatgttga atatataggg
     1021 gtggatatcc ccgcagaaga tctggccatc tacctcgaag aagttgaggt agatgtcgtt
     1081 gcattttctg tagttttacc tggtaatatt atgagcgtag aggacgctgt ttcaaccatc
     1141 gaatctttgg acaatccacc ctttgtaatt ttgggcgggc ccgctttcaa aggatttagt
     1201 ggtgaaatcg ggaaggcggt cattgtagac aattttgcta cggggctaac agttttgcag
     1261 gatgctatgg gaataggggc aggcaatttg tcattacagg atacacttct aggtgtaggt
     1321 ggaaaagttt tggagattcg aagagccaaa ggtatgaatc aaggtgaatt agcggttctt
     1381 gcaggggttg atagagccta tataagcctg gttgaaaacg gtaaacaaaa tctaactatg
     1441 gcagctttat tcaaacttgc ggaagccttg ggaacgtcag taaatacatt gttggcgttt
     1501 ccagcaaacc gaatcttctg tccaaaagat taatcaacac aaactggata agaattactc
     1561 tgtggacttt gaaatccttt ttaggactgg gcattgtggt ttcattggtg ggggtttaac
     1621 tccctgtatt ggaattgaaa tttaactaac accgcgccag ctaagatgat tacagtcgtt
     1681 gtggcgatca aatcaaaatg taccagccat ccggcaatta ttgtggatac aaaagcgaaa
     1741 acaccggtgt atgcaggtct tcttgtaatt attaatatca agaaagaagg taaaactgct
     1801 attaaggcgc cgaccgggag tagaacaatc ccggccccca tagtggtggc cattccgatc
     1861 cccccgggtt ttccccatgg ccgtataatc atatgtccta taaggaagaa gataattcca
     1921 atagttgcaa tccaggttgg gtacttgaaa aaaatcactg ggagagtgac tatcaaacct
     1981 ttggctacat ctataaaaaa aaccccaact ccatatatgg gtccgacctc tgcataaata
     2041 tttgaggcac caggattacc cgttcctaca gaacgaatat cgacattttt taattttgat
     2101 gtcaagtctc ccacagaaac ggtcccaatg aaatacgatg cgactgccca tatagctgat
     2161 aggatgatga tgttcaaatt ttcaatgctc ttgctggatg ttctgatgat tcttgttaaa
     2221 cgttatcaca ggcttacaaa aaccataaat gcacacaata attaaaatat tatgaaacgg
     2281 caaaaatccc aaaaattgat ggatgatcgg gatagaataa atattcctcc tgaagttgta
     2341 aaagataaat cgataaccaa aggactactg gggtgcttac cattcatttc gtcttcgata
     2401 tttttgcacg caagttttta ttatagaaac aatgtcgttg cagatcattc cttgttccat
     2461 tcaaatcctg aaatattgtt ttggacggca attctagtat gggcttttag ctttctggca
     2521 atttttttat tcggctatca ttcgttatca atggtcgaac gaaaacattt tcatagtatc
     2581 gttcctttag gtttggtggt aacgatcgca attacatgta catgtattaa tatttatatt
     2641 ctttcgtact acaacgtcct gtgaattaat cattccgtac gtataaagag cgagttgctt
     2701 ggtcatccta ctaactttgg taggaaaaac gggaatctgc taaccatgac actaacccca
     2761 aaagggaata atcggaccca aggaaccata tccaatcttt cctagagttt tcacaaaggc
     2821 atttactcag gaggtgtgtt ggctggatgg gtgtatggat aaattctcta ggattaacgt
     2881 taaagtaaat gggatttcat atgaaaacaa aaccattaat tacagttggg taggagagta
     2941 tctaggcatg gttctagact aggatcctaa acttggcgta tatagcggaa taatatatct
     3001 ctcatttggc tctttcatac ctccttaacc ttaggtaaaa cagtattaaa atactaatat
     3061 tagttatttg attccaccta ttgacactac tgtttcatat tgtctaaact atcttatcgt
     3121 gcatactaat tattttactt agctaatact tatttatata agatatatat acgaaataaa
     3181 taatattagc agacattgtg tattaattag tattataaag gttaaataat gtcatgggat
     3241 aacagaacaa ataaggagta agtggtgatc atcaatagga acatttttac aatgacgctg
     3301 gtcatactaa ctcttaccgc agtatttacg gcttgcacca cagaagtagt gaaagaggtg
     3361 cctgtagggg tgacaatcga gaaagaaatt gtcaaaaccc tggaagcccc cgtagtcgaa
     3421 aacacagtaa aagatcttga tcccggggaa ttaattctct attccgggag gtctgaaagt
     3481 cttgttggta acatagtcgc acagtttgaa gaaatgaccg ggatagacat acaggtcaaa
     3541 tatggcaaaa catttccggt tgctaccatg attcttgaag agggttccaa cagccctgct
     3601 gatgtgtatt tcgcacaaga tcccgggggg ttgggttttc tagccaaaga aggacggctc
     3661 atagaacttc ccgataccat aacagacagg gttgctgatt gggcgaagcc aaatgacggt
     3721 aaatgg
//
LOCUS       DASCNS010000078         1729 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000650974, whole genome shotgun sequence.
ACCESSION   DASCNS010000078 DASCNS010000000
VERSION     DASCNS010000078.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000650974"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /gene="miaA"
                     /locus_tag="QF838_02995"
     CDS             complement(<1..520)
                     /gene="miaA"
                     /locus_tag="QF838_02995"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HJO59692.1"
                     /translation="MIVILGATGTGKSQIGINIAHQLNGEIINADSRQIYKGMTIGTA
                     KPNEQDMKSIPHHLFDFKYPDESFSVFEFKELVSNTAHEIQSRGKVPILVGGTGQYLL
                     AFLENWTLNGGPPNPEIRKTLSDRLSKFGLDNLVEELTNTSPDIAQTIDLDNSRRVMR
                     ALEKIHNDDRHTL"
     gene            complement(517..1263)
                     /locus_tag="QF838_03000"
     CDS             complement(517..1263)
                     /locus_tag="QF838_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase family protein"
                     /protein_id="HJO59693.1"
                     /translation="MYILANWKNKLSSESSKSLANDIYSRLVYQYLNIEIVIFPDDIS
                     LLGVKSILDSLSIVKIGAQDGPLDRTSSLTGSISAPNLSNVCDYVLLGHSERRIQLGE
                     TNEMVALKATVAIELGLVPVICVTKGTGTALDPYSDIKAQLESIQDHIRRQGKYLIAY
                     EPIDAIGTGISADPQEIKNTAETIRKMLSGLQGSSDIPVLYGGSVNAKNVNSFLSLEG
                     IDGVLVGGESLNVDSFLKIVQKASDTQHSR"
BASE COUNT          468 a          326 c          348 g          587 t
ORIGIN      
        1 caagtgtgtg tctatcatca ttgtggattt tttctagtgc cctcatgact cttcttgaat
       61 tgtctaaatc gatggtctga gctatgtctg gggaagtatt ggttaattct tctactaggt
      121 tatccaaacc aaattttgat aagcgatcag atagcgtttt tcttatctct gggtttggag
      181 ggccaccatt taaagtccaa ttttccaaga aagccagaag gtattggcct gttcctccaa
      241 caaggattgg aaccttgcct ctagactgta tctcgtgcgc ggtgttggac accagttctt
      301 tgaactcaaa aacactgaaa ctttcatcgg ggtatttaaa atcgaataaa tgatggggaa
      361 tggacttcat atcttgttcg ttgggtttag ctgttccaat agtcattcct ttgtatattt
      421 gacgactatc ggcgttgata atttcaccgt ttaattgatg agcgatattg attccgatct
      481 ggctttttcc tgtacctgtc gcccctagta tcactatcat cttgaatgct gtgtatctga
      541 tgccttctgt acaattttta ggaaactatc cacgttgagg ctttctccac cgaccaagac
      601 tccatctatt ccttcaagcg acaagaatga gttcacgttc ttggcattta cactcccccc
      661 atatagcacc gggatatcag aagagccctg aagtcccgaa agcattttcc ggatggtttc
      721 ggctgtgttt ttgatttcct gggggtcggc agaaattccc gtgcctatgg cgtcgattgg
      781 ctcatatgcg attaaatatt tgccctgccg acgtatatga tcctggatag actctaattg
      841 ggctttgatg tcagaatatg gatcaagggc tgtcccggtt cctttggtta cacatataac
      901 tggaaccaaa cccagctcaa ttgctacggt agcttttaga gcaaccattt cattcgtttc
      961 ccctaattgg atccttcttt cagaatgtcc aagtagtacg tagtcacaaa cattgcttaa
     1021 attaggggca gaaatagaac cggtgagact actggttcta tccaaaggac catcctgagc
     1081 acctatcttg acgatagata gcgaatccaa aatagacttt actcctagca acgagatatc
     1141 gtctggaaat ataactattt caatatttaa atattgatat accagtcgtg agtatatgtc
     1201 attggccagg cttttactgg attctgagga caatttattt ttccaatttg ctaagatata
     1261 catatatttt gatatctccc agtatttaaa aggtgcttct ggtgctactt taacatccta
     1321 ctaaatattc cattggaagg cattgtttta cttttgagaa ggtttaaata tcaaacttta
     1381 ggggtttgag ccctaatttt gatgcatggt cactgcatta tgtcctgtag ttaatttatt
     1441 gtttagtttt gattaattat ggccatttta atcaacatat caggccactt ttggtggttg
     1501 ttcaatcggt aatagaggta gtgaaaggaa ttaggaatta tagaaaaaac agggtacata
     1561 tatggtgatg agtattgatc atggaaaaaa atccgccctg ggatgtttca ttacctagaa
     1621 atggtgcaaa tttggatgct caattgagct aattatattt gtaatttaac caaatcgtat
     1681 attaagaact taaatcaatg gtatattagt gatggctatg tttttatgt
//
LOCUS       DASCNS010000079         3045 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000652499, whole genome shotgun sequence.
ACCESSION   DASCNS010000079 DASCNS010000000
VERSION     DASCNS010000079.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000652499"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(42..917)
                     /locus_tag="QF838_03005"
     CDS             complement(42..917)
                     /locus_tag="QF838_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012966.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HJO59694.1"
                     /translation="MNWKEIYDRGSSVFGGAVSGGLDSCTVTHWLHQNGVNVHCFTVD
                     LGQPDEENLQAVANRMMACGASEATIVPGKEMLAEAGLKVLQAQARYEGGYWNTTGIA
                     RPITVKAILRKLQDHDINVLFHGATGRGNDQVRFQLASNMLQPEMEVYAPWRDQEFLN
                     KFPGRQQMIDYCELNKLPIRPARESRYSTDANFLGLTHEAGDLEDVAISPDFVEPGMG
                     VWPWDAPDRPEYVTIDWKEGVPTALNGSRLDLIEMFQEANSIAGRNGVGIGTHVIENR
                     FVGIKSRGIYESPGM"
     gene            complement(929..2125)
                     /locus_tag="QF838_03010"
     CDS             complement(929..2125)
                     /locus_tag="QF838_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HJO59695.1"
                     /translation="MTSTAEWKELESKYYMFLVRRQPMVLERGEGARVWDVDGKEYLD
                     FTSGWAVNNAGHSNPVIAEAIAEQAKTLVQTSNQFYTIPQLKLAEILVENSCLDKIFI
                     CNSGAEANEGAIKLAKKYGKKHKDGAYHIITALNSFHGRTMMNVSATGQPHYQELFQP
                     IPTGFSHVPYDDLDAVKEATTDNTVAVMIEPVQGEGGVNVPSVEYLKGVRDWCDQNGM
                     LLIFDEVQTGLGRLGTLFGYQSFGVEPDIITLAKGLGGGVPIGAFLAKDSACAFDPGD
                     HGSTFGGNPLTCAAAHASTKYIIDNEIPENAAKMGEYLGEGLRKIQSNNEFITDVRGM
                     GLIWAVEFDSDITPDVIASSNEAGLLMNPMRPNAIRLMPVLTITKEEIDEALSRLEAG
                     LSNASK"
     gene            complement(2127..>3045)
                     /locus_tag="QF838_03015"
     CDS             complement(2127..>3045)
                     /locus_tag="QF838_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015644.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HJO59696.1"
                     /translation="IKIVMNAYTGDVAFYIWDTTDPIALTYQKMFPTLFSDRDSMPSD
                     LVSHVRYPQGLFAIQAEKYIKYHMEKPQHFYGNEDLWAFPQEKFGQQEGLQPLEPYYV
                     IMRLPGETKEEFVQLIPYTPDGRPNMVGWLAARSDTTEYGKLIAFNFPKDRQVDGPEQ
                     VEARIDNDQDISAWFTLRCTEGSTCIRGNLLVIPIGNSILYAEPIYIQAEGVSFPELK
                     KVILSTADKVVMGDSLNDALYQITGTKDTIAMPQGDVKKETMSTPANLEGEGNSKLNE
                     IRNSIENLKAELDTIESLFENLEEVIKGD"
BASE COUNT          806 a          757 c          570 g          912 t
ORIGIN      
        1 aaaacgaact gcaaaagata ttcccaactt tgcccaagta attacatacc aggggattca
       61 tatatgcctc tagatttaat ccccacaaat ctgttttcta tcacatgagt gccaatgccc
      121 accccatttc tacccgcgat ggaattggct tcttgaaaca tttctattaa atccagccgt
      181 gatccgttca aggctgtagg aaccccttcc ttccaatcaa tggtcacgta ttctggtctg
      241 tccggggcat cccaaggcca aacccccata ccaggttcca caaaatcagg gctaattgca
      301 acgtcttcaa ggtcaccagc ctcatgagtc aaacctaaga aattagcgtc cgtagaatag
      361 cgggattctc gagctggcct tatcggcaat ttgttcagct cacagtaatc aatcatttgc
      421 tgtcgtcccg gaaatttatt aaggaattct tgatctctcc acggagcgta tacctccatt
      481 tcaggttgta gcatgttact ggccagttgg aaccggacct ggtcgttgcc ccggccagtg
      541 gctccatgaa aaaggacatt tatatcatgg tcttgaagct ttcgcagaat agccttaaca
      601 gtaataggac gggcaattcc ggtagtattc caatagcctc cttcatacct ggcctgtgcc
      661 tgcagtacct ttaggcctgc ttcggcaagc atctctttcc caggcactat agtagcctca
      721 gaagctccac aagccatcat cctgtttgcg acagcctgca aattttcctc atcaggctgg
      781 cctaaatcca ctgtaaaaca gtgaacgtta acgccatttt gatgcagcca gtgtgtgaca
      841 gtacaactgt ccaggccacc ggaaacagcg cccccaaaca ccgaggaccc tctatcgtat
      901 atctccttcc aattcacctc agcctcgttt atttcgaagc attggacagg ccggcttcca
      961 atctggacaa ggcttcgtct atttcttctt tagttatagt caaaacaggc ataagtcgaa
     1021 tcgcatttgg cctcataggg ttcattaaaa gaccagcttc attacttgag gcaataacgt
     1081 cgggggtaat atcggaatca aattcaaccg cccaaatcag gcccattcct ctaacgtctg
     1141 ttataaattc gttattcgat tgtatcttcc tgagtccttc cccaagatat tcacccattt
     1201 ttgccgcgtt ctcgggtatc tcgttatcga tgatatattt tgttgaagca tgcgctgcag
     1261 cacaagtcaa tgggttacct ccaaaagtgg aaccatgatc acccggatca aaagcacatg
     1321 cagaatcctt agccaaaaat gctccaattg gaaccccgcc gcctaggcct tttgcaagag
     1381 taatgatgtc gggttcaacg ccaaaacttt ggtatccgaa caaggtacct aatctcccca
     1441 atccggtctg cacttcgtca aaaatcagca gcattccatt ttgatcgcac cagtctctta
     1501 ctcccttcaa atattcgaca gaaggaacgt ttacccctcc ttctccttga acgggctcga
     1561 tcatcacggc gacagtgttg tctgttgtag cctcttttac tgcatctaaa tcatcatatg
     1621 ggacatgtga aaacccggta ggtatgggct ggaaaagctc ttggtaatga ggctgacccg
     1681 tggcagagac attcatcatg gtgcgaccat gaaatgaatt aagagccgtt attatgtgat
     1741 aagccccgtc tttatgtttt ttcccatatt tttttgctaa tttgattgca ccttcgttag
     1801 cctctgctcc actgttgcag ataaatatct tgtctaggca gctattttca accaaaattt
     1861 cagctaattt taactgagga atggtataaa actgatttga agtctgaacc aaggtttttg
     1921 cttgttcagc tattgcttcg gcaataactg ggtttgaatg ccctgcatta ttgacagccc
     1981 acccagatgt gaaatccaag tattcttttc catcgacatc ccatactctc gcaccctctc
     2041 ctctttcaag taccataggc tgacgcctaa caagaaacat atagtattta ctttcaagtt
     2101 ctttccattc ggcagtgcta gtcatatcaa tctcctttta ttacctcttc caagttttca
     2161 aacaaagatt caatcgtatc taactcagct ttcagatttt caatagaatt tctgatttca
     2221 tttaacttac tatttccctc accctctaaa tttgcaggtg tagacattgt ttcctttttt
     2281 acatctcctt gaggcatcgc aatagtgtct ttcgtaccag tgatttgata caaggcatcg
     2341 ttcagtgagt ctcccataac gaccttgtct gctgtagaca aaataacttt cttaagttct
     2401 ggaaagctaa ccccttctgc ttgaatatat atgggctcag cataaaggat actattcccg
     2461 attggaataa ctagcaggtt acctcttata catgtagacc cttcagtaca tctaagcgta
     2521 aaccaagcag agatgtcttg atcattatct attctagctt ccacttgttc aggtccatcg
     2581 acttggcgat ccttaggaaa gttgaaggct attaatttgc catattcagt ggtatcactt
     2641 cttgcggcca gccaaccaac catattaggt cttccatcag gagtatatgg aatcaattga
     2701 acaaattctt cttttgtctc ccctggaagc cgcatgatca cgtagtatgg ctccaacggt
     2761 tgcaatcctt cctgttgccc aaatttttct tgtgggaaag cccataagtc ttcattccca
     2821 taaaaatgtt gtggcttttc catgtgatat ttaatatatt tttcagcttg gatggcaaag
     2881 agtccttgtg gataccttac gtgtgaaacc aaatccgaag gcattgaatc gcgatctgaa
     2941 aatagggtag gaaacatttt ttgataggta agggcaatgg gatccgtcgt gtcccagata
     3001 taaaacgcta catcccctgt atatgcattc atgactatct tgata
//
LOCUS       DASCNS010000080        11320 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000655341, whole genome shotgun sequence.
ACCESSION   DASCNS010000080 DASCNS010000000
VERSION     DASCNS010000080.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000655341"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="QF838_03020"
     CDS             complement(<1..1073)
                     /locus_tag="QF838_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HJO59697.1"
                     /translation="MELIQIVALVASLVSLAFAAFLAYAVVGKDEGSDQIRFIGKAIQ
                     EGAMAFLSREYRLLAIFVVIMAVILAVFVDLDVTGRMDKQSTSSIPVGTAIAYLVGAF
                     GSGLAGFIGMSIAVRANTRTTVQAMQGLNSALRVAFNSGAVMGMSVVGLALLGVTLML
                     VVFSNDYSIVAGFGFGASSIALFARVGGGIYTKAADVGADLVGKVEQGIPEDDPRNPA
                     TVADNVGDNVGDVAGMGADLFESYVGSIIATVAIAAVGASFLGADTLKLQLFPLLVAS
                     IGIISSIIGTFLVRTKEGADMGRLLWALRTGIFSAGALVLIGTGGLIVLLDLNFNLFW
                     VVLIGLVAGQLIGTASEYYTSYE"
     gene            complement(1216..2118)
                     /gene="thiE"
                     /locus_tag="QF838_03025"
     CDS             complement(1216..2118)
                     /gene="thiE"
                     /locus_tag="QF838_03025"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HJO59698.1"
                     /translation="MPELLVSICQEISDFIPNNEKDLKTELGFIVGTSKLSKSSTAVP
                     EKPEKALNLCAAALAIIGIGDGGNKKAKEILLKIESYFFLQIRLSIRNKMDGIYIIVD
                     PEATNNIDVYDMTKSSLEGGVKVVQYRDKLNDRAPFLENCYKIKELCDQYNAAFVVND
                     AVDIARLSGADFLHVGQSDMPVEEARRILKPSQCIGRSNGGVSESTESEEFGVDYLAV
                     GAIYATSTMGKSARSPLGPSMITEVKSQTGLPIVAIGGITIENLPDVIQAGADSVCMV
                     SAITMSEDPEAAARQLVNIWDRYR"
     gene            complement(2133..2450)
                     /locus_tag="QF838_03030"
     CDS             complement(2133..2450)
                     /locus_tag="QF838_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJO59699.1"
                     /translation="MPIYEYQCKDCKDDFELFFKSFNFTQDPVCPSCGSIEVGKKISR
                     VTFKFGGSFDKSESDNYYSDASNIGKNVEASFSKHGVDMPDSVRKSIDDARGGKMPDG
                     LDL"
     gene            complement(2453..3076)
                     /locus_tag="QF838_03035"
     CDS             complement(2453..3076)
                     /locus_tag="QF838_03035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HJO59700.1"
                     /translation="MTSLEIERKLISQGYRLIGGIDEVGRGCLAGPVAAGFVIFPPYV
                     DDSLLSSVTDSKKLTAPKREHLLKAIKSESLCAEVGWASVAEIDELGIAVATKLAMMR
                     AMEKSIIQPDYLLVDAVKLDECPVPYEAVVKGDLISRTIAAASIIAKVTRDLHMSSLS
                     MEYPGYSLEKNKGYGTRDHMIAIKSRGPTDIHRGSFEPVASMKRYPG"
     gene            complement(3101..4225)
                     /gene="mnmA"
                     /locus_tag="QF838_03040"
     CDS             complement(3101..4225)
                     /gene="mnmA"
                     /locus_tag="QF838_03040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HJO59701.1"
                     /translation="MKKEKVIVAMSGGVDSSVAALLLKNQGYDVVGMTLRLWTEDSEN
                     SNGANNRCCSVEDVEDARRVCQMLDIPHYFVNFEKEFQEHVVDYFVSEYDSGRTPHPC
                     LACNDKIKFDFLLRRALFLDADYIATGHYARLAAADGNFHLLKGIDGSKDQSYVLYTL
                     TQQELSRLKFPVGEYTKLDIRKLAEEAGLLVAGKPDSQEICFIPSGNYRDFVKERVKP
                     KTGNYIDTDGNILGEHPGIQFFTVGQRRKLGINPNDGEPRYVIKINSESNEVVLGADE
                     DLMETDFTANKLSFTSKVEVDAEMEVKARIRYKAKEEPALVILRDGYADIRFVEPQRA
                     ITPGQPVVFYQDKEMIGGGIIEDLVVNAPINSNSETITVF"
     gene            4305..4742
                     /gene="rsfS"
                     /locus_tag="QF838_03045"
     CDS             4305..4742
                     /gene="rsfS"
                     /locus_tag="QF838_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HJO59702.1"
                     /translation="MKNTGENSGSTSAELEIGHASDLNEDNIRPIDHGRSASDLAVES
                     AANLQAENIHLLDVRGLSDFTDYFVILTATSSRHLRAVASDIEFSLKSEGHVIHHKEG
                     DHTSGWTLLDYGNLVVHLFAPDYREFYNLESAWSEAKTLRIIQ"
     gene            complement(4732..5181)
                     /gene="ndk"
                     /locus_tag="QF838_03050"
     CDS             complement(4732..5181)
                     /gene="ndk"
                     /locus_tag="QF838_03050"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HJO59703.1"
                     /translation="MEKTLILVKPDGVQRGLVGEIIGRLERKGLRIAGLKMVHVNEEL
                     ANKHYGEHVGKPFFDSLVKFITSSPLVAMALEGENAVEVARNLMGSTNPKEAAPGTIR
                     GDYGLTIGMNLIHGSDSLQSAERELSIFFDESEITDYDRAIDQWIIE"
     gene            complement(5192..5875)
                     /gene="tsaB"
                     /locus_tag="QF838_03055"
     CDS             complement(5192..5875)
                     /gene="tsaB"
                     /locus_tag="QF838_03055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HJO59704.1"
                     /translation="MELCIDTSTRYAMVGISEKGELLQELTWHSQRNHSVELAPAIES
                     VLSRQQISVKDLAGVFVAKGPGAFSALRVGMSVAKSLSAGVGIPIVGISTTDIEVEPY
                     LGVSQNIIGLVPAGRERVYIATYVDGDVEQRDIIVRNYDQIDFDKTDDTFFCGEGVKG
                     ILESQYADIIGGKYARDVSPTRAASVLMKLGYGKFESGEFDDPISLEPIYVRSAQIES
                     ADNSVKNKS"
     gene            complement(5880..6356)
                     /gene="tsaE"
                     /locus_tag="QF838_03060"
     CDS             complement(5880..6356)
                     /gene="tsaE"
                     /locus_tag="QF838_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HJO59705.1"
                     /translation="MTLTIVSTSESNTIKIGEIIGSFIVPGDVILLSGDLGSGKTKMT
                     QGIIKGIGSDDFARSPTFVLITEYEAKFPIYHMDLYRLDGVENLERMQIDEYLYGDGV
                     CIVEWADKANKSFPNKSLTVDFSFISEYQRKIDIEWPSDRLDKIGGEITRSISRVN"
     gene            6430..7095
                     /locus_tag="QF838_03065"
     CDS             6430..7095
                     /locus_tag="QF838_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014305.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59706.1"
                     /translation="MNFEQINETALPTVIGTPDNYDPTQEYGMVVLMHGFGSHMGDLA
                     GLAPLINETDYIYVCPNAPIEMTIGFGQQGYAWFPAGGQTEPDDIDKAVSQLQKSVDF
                     TIDKYRPTQSNIYIGGFSQGGMMTMHAGLTRPDIYKGAIILSSRLTQEDLFTDRIVDL
                     DKIPIFMSHGTNDLVISIEDGRDTKELLDEYGYQIEYREYPMAHEIREETISHLKDWL
                     SKN"
     gene            7128..8162
                     /locus_tag="QF838_03070"
     CDS             7128..8162
                     /locus_tag="QF838_03070"
                     /inference="COORDINATES: protein motif:HMM:NF028222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO59707.1"
                     /translation="MKAAFYNGNGKMQIKEHPDPTPESGDAIIQVRATGICGSDLLMN
                     NDKEEADELPTGHEVTGQIVEVGSEVDRNLIGQRVAIETIGQGRACSTCWYCRMGQYR
                     QCQNMAVVEGGGFAEFIRRKAIGCYQIGESMTWEEGALVEPLAVSIHGIRRGLMNGAE
                     TVAILGSGTIGLTAVVAARQLGAGKIFVTARHKQQANMALALGADYACDPSNGDFERL
                     ILENTEGRGADLTVETVGGKSNATLTQSVKSTRMQGRIVILGGFRTPLEFDWLQPLLK
                     EQSFIFSSCYGVLNGYHDYEMAIELLSQKENKLSEIVTHKFSLKDIQKGFECAYDKTT
                     GSIKVQIHQE"
     gene            complement(8187..8813)
                     /locus_tag="QF838_03075"
     CDS             complement(8187..8813)
                     /locus_tag="QF838_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59708.1"
                     /translation="MRRETVAEATVSTSLKEKKSMGMKENFRDKSRDELTWNLTNLAG
                     VKAQMSERERPEEKVENSWYQRSLGVIDITDGPIKWISILKRDRSKDSPPKWWVVMGI
                     PFDGDGTTGQKISIKTVRKKAFPLFGKVVDVNWKGDDGVSGLINTLSQDTETKELSKK
                     IGNLEIKSQSEGFQGWTLTSDKRFAPTSQDWEVIQGIANYILSAPRAF"
     gene            complement(8851..10560)
                     /locus_tag="QF838_03080"
     CDS             complement(8851..10560)
                     /locus_tag="QF838_03080"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HJO59709.1"
                     /translation="MSTDLPPNRSREWFDTPELYGWLRRAGFKAEGFSESSYEGKPII
                     GICNTWSELTHCNSHLRDLAQSVKIGVWQAGGFPMEFPVMSLGEYNMKPTTMLYRNLL
                     SMDVEESITANPLDGVVLLGGCDKTTPALLMGAASADIPAILVTGGPQLKGNWKGEEL
                     GSCTDCRRYEVELRAGTIDEDDWAELQSCIVRSNGHCMTMGTASTMGTMGEALGMSLP
                     GNAAIPAVDSRRKQHAEEAGRQIVKNVNSGLIPSRIMDEKAFDNAIKCLHAIGGSTNA
                     IVHLTAIAGRVGIDLSLERFDELSKTTPFLLNLKPSGQYLMEDFYYAGGVPALMGRIE
                     SILDLGNITVTGRTLGENIAGHLVHNEDIIRPMSNPLDPEGGLAVLYGNIAPTGAVIK
                     PTAASPGLMVHTGKAIVFEDHDDLGNRIDDPDLEVSPDDILVMKNSGPIGGPGIPEWG
                     FLPIPKKILATGVRDMVRLSDARMSGTAFGTVVVHVTPESAGGGPLSAIRDGDMIEID
                     VPNRKLNLLVTDEELAERLNVHKNGAPDFKRGYKWLHAQHILQADKGCDFDFLRAESL
                     QSD"
     gene            complement(10557..11231)
                     /locus_tag="QF838_03085"
     CDS             complement(10557..11231)
                     /locus_tag="QF838_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59710.1"
                     /translation="MATLGPTGKADPQQFDRKRKLFLVPLVTVPPQLEEQISELADTH
                     WDQVKAQLQNLEKSLGTIRHVFHELIHDEGKAGEQLLSQIAPRSASLIKEITDSSGHL
                     EQTEDQDLLFELSDWQRCLSMGLVSQKVSDLASENYQTAMSDRYEYIGKQIDETLEED
                     GVAALFISEDHRVQFPSDIQVFYVSPPSQNQLKQSIVGLIQKSFEESQIEPDEEVSNN
                     TDKEVE"
BASE COUNT         3208 a         2691 c         2105 g         3316 t
ORIGIN      
        1 tattcatatg aagtatagta ctcagaggca gtacctatta attgccccgc taccagacct
       61 atcaacacga cccagaaaag gttgaaatta agatccaaaa gaactatcaa tcctcctgtt
      121 ccaattaaga caagggcacc agctgaaaaa attccagtcc tcaaggccca taggaggcga
      181 cccatgtcag ctccttcctt tgttctaact aaaaaagtac cgattattga agaaatgatt
      241 cctatagatg caaccaaaag aggaaacagc tgtaacttta aagtgtcggc cccaaggaac
      301 gatgcaccta ccgctgctat tgcaacagta gcaattattg accctacata ggactcaaag
      361 agatcagccc ccatacctgc aacgtcacca acattatctc cgacattgtc ggctacagta
      421 gctgggttac ggggatcatc ctcgggtatc ccttgttcta ccttacctac caagtcagcc
      481 cccacgtcag cggccttggt ataaatccct ccgcctaccc tggcaaacaa ggctattgat
      541 gatgctccaa atccaaaacc tgccactata gagtaatcat tgctaaaaac aacgagcatt
      601 aaagtcaccc ctaagagtgc aagccctacc accgacattc ccatgactgc accactgtta
      661 aaggctaccc gtaaagctga gttcaaacct tgcattgctt gtacggtggt tcgggtgttc
      721 gccctgacag ctatgctcat acctataaag ccagccaatc ctgaaccgaa agctccaact
      781 aaataagcaa tcgcagtgcc aacaggtatt gaagaagtgc tttgtttgtc cattctaccg
      841 gtgacatcga ggtcgacaaa cacggctaga ataactgcca taatgacaac aaatatagct
      901 aaaagacgat attctcgcga tagaaatgcc atcgcccctt cttgaatggc cttaccgata
      961 aatcggatct ggtcactacc ttcgtctttt cctacaactg cgtacgctag gaaggccgca
     1021 aaggctaaag agactaatga ggcaacaaga gcaacaatct gtatcagttc cattgtatcc
     1081 tcctacatcc atggaaaatt tctgtgcaaa aacacacaat aaggtcaagt ttgcacaagg
     1141 tttaattttc ttctctggaa aatccgcggt caatatagat gaggcccata atatgagtaa
     1201 taatcaatat taaccttatc tatacctgtc ccaaatattt accaattgac gcgcggccgc
     1261 ttccgggtct tcagacatag ttatggcgct caccatacat acagaatcag cacctgcctg
     1321 aataacatcg gggaggtttt caatggtgat acctccgatg gcaacaattg gcagtcctgt
     1381 ctgtgatttt acctctgtaa tcatggaagg cccaagtggg cttcttgctg acttacccat
     1441 cgtagaagtt gcatatatgg ctcctacagc aaggtagtca acaccgaatt cttcagactc
     1501 tgtcgactct gacacccctc cgtttgatct accgatacat tgagaaggtt ttaaaattcg
     1561 cctggcttct tcaacaggca tatctgactg gcccacatgg agaaaatccg cgccacttaa
     1621 tctggctata tcgaccgcgt cattcactac aaaggcagca ttgtattgat cacaaagctc
     1681 tttgattttg taacaatttt ccaaaaacgg agctcgatca ttcaatttat ccctgtattg
     1741 gactaccttt acgccaccct ctaatgaaga tttagtcatg tcatagacgt ctatattatt
     1801 ggttgcctcc gggtccacta ttatgtaaat gccatccatc ttatttctta tcgatagcct
     1861 tatctgtagg aaaaaataac tctcaatttt gaggagaatt tccttcgctt ttttattacc
     1921 accatcacct atcccaataa tagctaaagc agccgcacac agattcagag ctttttcagg
     1981 cttttcaggt actgcagtac ttgattttga taatttactc gtcccgacta taaaacctaa
     2041 ttcagttttt aaatccttct cattattggg tatgaaatct gatatttcct ggcatataga
     2101 aactaaaagt tctggcatat attatgtttc ctttatagat ctaatccgtc cggcatcttg
     2161 ccccctcggg catcatcaat agatttccta acgctatctg gcatatccac accatgtttt
     2221 gaaaaagaag cttcaacatt cttccctata ttggatgcgt cagaataata attatccgat
     2281 tcactcttat caaaggatcc tccaaattta aaggttaccc gagatatttt tttaccaacc
     2341 tcaatcgacc cgcacgaagg gcaaactggg tcttgggtga aattgaaaga cttgaagaac
     2401 aactcaaagt catctttaca atctttacat tgatactcgt atattggcat ttttaacctg
     2461 gatagcgttt catactagct accggctcaa agctccctct atgaatgtca gttggccccc
     2521 tagattttat agcaatcata tggtctcttg tcccgtagcc tttatttttt tcgagggagt
     2581 atccagggta ttccatactc aaactagaca tatgaagatc ccgagtgact ttagcaatga
     2641 ttgatgctgc ggcaattgtc cttgatatta agtctccttt tacaacagcc tcatatggaa
     2701 ctgggcattc atccaattta actgcatcaa cgagtaaata atctggctga atgatggatt
     2761 tttccatagc ccgcatcatc gccaattttg tagcaacagc aatacctagt tcatctattt
     2821 cagcaacact ggcccaacca acttcggcac ataaggattc agattttatt gcctttagga
     2881 ggtgctctct tttcggagca gtgagttttt tactgtcagt gacagaagaa agtagtgagt
     2941 catcaacata cggaggaaat atgacaaacc cggctgccac aggcccagcc aggcaccctc
     3001 tcccaacttc atcaatacca cctatcaaac gataaccttg ggaaattaat tttcgctcga
     3061 tttcaagcga ggtcaaaagt atcgtcacct ctaagctgaa ttagaaaacg gttatagttt
     3121 cactgttgct gttaatggga gcattcacca ctaaatcttc gatgatccca cctccgatca
     3181 tctccttatc ttgataaaat actacgggtt ggccgggagt tatagcccgc tgaggttcaa
     3241 caaatcttat atctgcataa ccatctcgaa gtatcaccaa agccggttct tctttagctt
     3301 tatatctaat tcttgccttc acttccatct cggcatcgac ttctactttt gaggtaaaac
     3361 tcaatttatt tgctgtgaaa tcagtctcca tgagatcttc atcggctccc agcacaactt
     3421 cattactttc agaatttatc tttatcacgt acctaggttc accatcgtta ggatttatgc
     3481 ctagtttacg cctctggccc actgtgaaaa actggatccc tggatgctct cccaaaatgt
     3541 ttccatcagt atctatataa tttcctgtct tgggttttac tctttctttg acgaagtcac
     3601 gataattgcc acttggaata aaacaaatct cctggctatc aggttttcct gcaaccagta
     3661 acccggcttc ttcagcaagt tttcttatat ccaacttggt atattcacca actggaaact
     3721 tcagacggct taattcttgc tgagtaaggg tatataaaac gtacgattgg tccttgcttc
     3781 catctatacc ttttaacaga tgaaaatttc catcggcggc tgcaagcctt gcgtagtgac
     3841 ctgttgctat gtaatcagca tcaagaaata gagctcttct caataaaaaa tcgaacttga
     3901 ttttgtcatt acaggctagg catgggtgag gtgtccgacc tgaatcatat tcactgacaa
     3961 aatagtccac cacatgttct tgaaattctt tttcgaaatt cacgaagtag tggggaatat
     4021 ctagcatttg gcagactcgt ctagcatctt ccacatcttc aactgaacaa catcggttat
     4081 ttgccccatt ggaattctca gagtcttctg tccaaagtct caatgtcata ccaacgacgt
     4141 cgtatccttg attcttcaac agtaatgcag caacggaaga atcgacccca ccactcatcg
     4201 caacaattac tttttccttt ttcatacaat taggctagca cacaaatggt caattgttat
     4261 ctataaaaac atcttttaga taaagaatat ataattctac ccttatgaaa aacacggggg
     4321 aaaatagcgg gtccacatca gctgaattgg aaattggtca cgcctctgat ttaaatgaag
     4381 acaatatacg acctattgac cacggtaggt ctgcttctga tttagcagtc gaatcggccg
     4441 caaatttaca ggctgaaaat atacacttgc tcgatgtgcg aggcttaagt gatttcaccg
     4501 attattttgt gatcctaacc gctacatctt ctcgccatct aagggcagtc gcctcagata
     4561 ttgagttctc gctaaaatcc gaggggcatg tcatccatca caaagaggga gaccatacca
     4621 gcggctggac attactggac tatgggaatc ttgttgtcca tctgtttgcg ccagattatc
     4681 gagaatttta caatttagaa agtgcgtggt cagaagccaa aactttgaga attattcaat
     4741 aatccattgg tctatggctc tgtcatagtc cgttatttcc gattcatcaa agaatataga
     4801 taattccctt tcagctgatt gcaatgagtc tgaaccgtgt attaagttca tccctatggt
     4861 caaaccataa tcccctctga ttgttccagg agcagcttct ttggggtttg tggaccccat
     4921 aaggttccta gctacttcca cagcgttttc tccctcgagt gccatggcaa ctagtggcga
     4981 agacgttata aatttaacta gggagtcaaa aaacggctta ccgacatgct ctccataatg
     5041 tttgtttgct aactcctcat taacatggac cattttaagt ccggcaatcc taagtccctt
     5101 tcgctcaagc ctgcctataa tttctcccac caatcccctt tgaacaccat caggtttcac
     5161 caatattaat gtcttttcca atagattttc ctcatgactt attttttact gaattatctg
     5221 ccgattcaat ttgagcactt cttacgtaaa taggctcaag tgaaattggg tcatcaaatt
     5281 ccccagattc aaatttacca taacctaatt tcatcaaaac cgatgcagcc ctagtgggag
     5341 atacatctct tgcatattta ccaccaataa tatccgcgta ttgggattcc aagatacctt
     5401 taacaccttc tccacaaaaa aatgtgtcgt cggttttatc aaaatcaatt tggtcatagt
     5461 tacgaacgat gatgtctctc tgctctacat caccatcaac atatgtagcg atatataccc
     5521 gctctctgcc tgcaggaacc agtccaatta tattttgaga tacgccaaga taaggttcca
     5581 cttcaatatc agtcgttgaa attccaacaa ttggaatccc gacaccggcg ctcaaggatt
     5641 tggcaacact catcccaacc cttaaggcgc taaatgcacc cggacccttt gctacaaata
     5701 ctccagccaa atcttttacc gatatttgct gtctacttaa cacactctct atagcagggg
     5761 cgagttcaac tgaatggttt ctctgagaat gccatgttaa ctcctgaagc aactcccctt
     5821 tctcagagat tccaaccata gcgtatcgag tggaagtatc tatacatagt tccattattt
     5881 taattcaccc ggcttataga tcttgtgatt tccccaccta ttttatccaa ccgatccgac
     5941 ggccattcta tgtctatttt tctttgatac tcagaaataa aggaaaaatc gaccgttaaa
     6001 cttttatttg gaaacgattt attcgcctta tcagcccatt ccacaataca aaccccgtca
     6061 ccatacaaat attcgtcaat ttgcattctt tcaaggtttt caacaccgtc taatctataa
     6121 agatccatat ggtatatcgg aaatttcgct tcatattcag ttatcagtac aaatgtgggg
     6181 ctcctagcaa aatcatcaga cccaataccc ttaataatcc cttgagtcat ttttgttttc
     6241 ccacttccca aatctccact cagcaatatc acgtctccag ggacaatgaa tgatcctata
     6301 atttcgccga tcttaatagt atttgattca cttgtgctga ctatagttaa agtcatagat
     6361 tcctgtcaca aaagggttga ttgaaataca ctacaactaa cgataccaaa tatatgtagg
     6421 gaaaacttaa tgaattttga acaaataaat gaaactgcct tgccaacagt tatcggaact
     6481 cctgacaatt atgatcctac acaggaatat ggaatggtgg tgctgatgca tggtttcggc
     6541 agccatatgg gagacctcgc gggattggca ccgctaatca atgaaaccga ttatatttac
     6601 gtctgtccaa acgccccaat cgagatgacc attgggttcg gccagcaggg ctatgcatgg
     6661 tttccagccg gcggacaaac agaacctgat gacattgaca aagcagtttc ccaacttcaa
     6721 aaatctgtcg attttactat agataaatat agaccaaccc aatccaatat ctatattggt
     6781 ggattctctc aaggcggaat gatgacgatg catgcaggcc tcactcggcc agatatttat
     6841 aaaggtgcca tcatattgag ctcccgactc acccaggagg acctcttcac tgacaggatc
     6901 gttgatttgg acaaaattcc catatttatg tcgcatggga caaacgacct tgtcatatct
     6961 attgaagatg ggcgtgacac aaaggagtta ttggatgaat acgggtatca aatagaatat
     7021 cgggaatacc ccatggcaca tgaaatcaga gaggagacca tatctcactt aaaagattgg
     7081 ctcagcaaaa actagataca gtaatttgga cttgataggg agtatgtatg aaagcggcct
     7141 tttataacgg aaacggtaaa atgcaaataa aagagcatcc tgatccaact cctgaaagcg
     7201 gggatgcaat tattcaggta cgggctactg gaatatgtgg ctcagactta cttatgaaca
     7261 atgacaagga ggaggcagat gaactcccta ctggccatga ggtcaccggt caaatagtgg
     7321 aagtaggatc cgaagttgat aggaacctta tcggacaaag agtagctata gagactatcg
     7381 ggcagggaag ggcctgttcg acatgttggt attgccgaat ggggcaatat cgacaatgtc
     7441 agaacatggc agtcgtagaa ggaggtggat ttgcggaatt catccgaagg aaagcaatag
     7501 gttgttatca aattggagaa tctatgacat gggaggaagg agcactagtg gaacctctgg
     7561 ctgtatccat acatgggatc agacggggac ttatgaatgg ggcggaaact gtggccattc
     7621 ttggttctgg cactataggt ttaactgctg ttgttgcggc aagacaactc ggtgccggaa
     7681 aaattttcgt tactgctcgt cataaacagc aggctaacat ggctttagcc ctaggggcag
     7741 attatgcatg tgatccgtct aacggagatt tcgagaggtt gatactggaa aacacagaag
     7801 gaaggggggc tgatcttacg gtagagaccg ttggaggaaa gagtaacgcc accttgacac
     7861 aatcggtgaa atccactcga atgcaaggca gaatagtgat attaggtgga tttagaacac
     7921 ctttagaatt tgactggctt cagcctcttc taaaggaaca atcttttata ttctcatcgt
     7981 gctacggagt attaaacggg taccacgatt acgagatggc aattgaacta ctttcccaaa
     8041 aagaaaataa actgagcgaa attgtcaccc ataaattctc actcaaagat attcagaagg
     8101 gatttgaatg cgcctatgac aaaactaccg gttcgatcaa agttcaaata caccaagaat
     8161 aatctccaga aataatcgaa tgtgttttag aaagccctag gtgccgaaag aatataatta
     8221 gctatacctt gtataacttc ccagtcttgg ctagttggag cgaatctttt gtctgaagtc
     8281 aatgtccacc cttggaatcc ctctgactga cttttaattt ccagattacc gatctttttt
     8341 gacaattcct tggtttcggt gtcttgagat agtgtgttta taagaccgct aacaccgtca
     8401 tcacccttcc aattgacgtc aactaccttg ccgaataaag gaaaagcttt ctttctaacc
     8461 gtctttattg aaattttttg gccagtagtg ccatcgccat caaatggaat ccccatcaca
     8521 acccaccatt ttggtggact atctttgctc cggtctctct ttaaaatgct tatccatttt
     8581 attggtccat ctgtaatgtc tataaccccc aatgaccttt gataccaaga attttccacc
     8641 ttttcttccg gccgttcacg ctcactcatt tgtgccttga caccggctaa attggtaaga
     8701 ttccaagtta attcatctct agatttatct ctaaaatttt ctttcatccc catagatttt
     8761 ttctccttca gagatgtaga cactgtggct tctgcaactg tttcccgccg cattaaatca
     8821 cagtaaaaac catccaaaat agctagatat ctagtcgctt tgcagtgatt cggccctcaa
     8881 gaaatcgaag tcacaccctt tatctgcctg taagatatgc tgggcatgaa gccatttata
     8941 accgcgttta aagtcgggcg ccccattttt atgaacattc agcctttcag ccaactcttc
     9001 atccgttacc agtaaattta gtttcctgtt aggaacatct atctcaatca tgtcaccatc
     9061 tctaatagca gatagaggtc caccacccgc tgattctggg gtaacatgga ccactacagt
     9121 accaaatgcg gtaccactca tccttgcatc acttaacctg accatatctc ttacgcctgt
     9181 tgccaaaatc ttctttggaa taggaagaaa cccccattca ggaatacctg gccccccgat
     9241 gggaccacta tttttcataa ccaaaatatc atcaggtgat acttcaagat ctggatcatc
     9301 tatacggttc cctaagtcat catgatcttc aaatactatt gctttccctg tgtggaccat
     9361 taggcctgga gatgccgcag tcggttttat caccgcaccg gtgggtgcta tattcccata
     9421 aagtactgcc aatcctcctt ctgggtcgag cgggttgctc atcggtctaa taatatcttc
     9481 attatgaaca aggtggcccg caatattctc acctaaagtt cgacctgtta ctgtaatgtt
     9541 ccctaggtcc aaaatgctct caattctccc cattaaggcg ggtactccac ccgcataata
     9601 aaaatcttcc atcaaatatt gtcccgaagg cttcaagttg agcaaaaatg gggttgtttt
     9661 agataattcg tcgaatctct ctaaggaaag atctatacct acccgaccag ctatagcagt
     9721 caagtgcact atagcgtttg ttgaaccgcc gatagcatgc aagcacttga ttgcattatc
     9781 aaacgccttc tcatccatta ttctcgaagg tattagacct gaattcacat tcttcactat
     9841 ttgccttcca gcttcttctg catgttgttt ccggcgagaa tcgacggctg gtattgcagc
     9901 gtttccaggc aatgacatac ctaaagcttc tcccattgtt cccatggtag aagccgtccc
     9961 catcgtcata caatgtccgt tgctcctaac tatgcaactt tgtaattcag cccaatcatc
    10021 ttcatcaatg gtgccagccc taagttcaac ttcatagcgt ctgcagtctg tacatgatcc
    10081 tagctcttcg cctttccaat tacctttgag ttgaggtcca cctgtgacca atatagctgg
    10141 tatatcagca ctagcagccc ccattagaag agctggggtg gttttgtcac atccgcctaa
    10201 tagaaccaca ccatccaaag gattggctgt tattgattct tcaacatcca tacttaatag
    10261 atttctgtaa agcatggttg tcggtttcat gttgtattca ccgagtgaca taacagggaa
    10321 ttccataggg aatcctccgg cctgccagac gccaattttc acggattgtg ccaagtctct
    10381 taaatgagaa ttgcagtggg taagttcact ccacgtgtta caaattccta ttattggctt
    10441 cccttcatat gaagattcac taaacccttc cgctttaaaa cctgccctcc tcaaccatcc
    10501 atatagctct ggcgtatcga accattctcg acttctgtta gggggtaggt cagtactcat
    10561 tcgacttcct tatcagtatt gtttgaaacc tcttcatcgg gttcaatttg actctcctca
    10621 aagcttttct gtatgagacc tactatcgac tgtttcaatt ggttttgaga tggaggagac
    10681 acgtaaaaga cctgtatatc cgacggaaac tggactcgat ggtcttctga tataaataat
    10741 gcagcgactc cgtcttcttc aagagtctca tcaatctgtt tgcctatata ttcataccta
    10801 tcactcattg ctgtttgata gttttctgat gccaggtcag ataccttttg acttactagg
    10861 cccatcgaga gacatctttg ccagtcagat aattcaaaca gtaagtcttg atcttcagtt
    10921 tgttctaagt gtcctgaaga gtccgtaatc tctttaatta aagaggccga cctgggcgca
    10981 atttgagata gtaactgctc tccggccttg ccttcgtcat gaattaattc atggaacacg
    11041 tgtctaatgg tgcccaaaga tttctccagg ttttgtagct gggctttgac ttgatcccag
    11101 tgtgtgtctg cgagttcgga aatctgttct tctaattgag gtggcacggt cactaaaggc
    11161 accaaaaaca atttcctttt tctatcaaat tgctggggat ccgcttttcc tgtaggacct
    11221 aatgtcgcca tttaatttct ctccaatctt accgcgcaaa ctttgtattc aggtattctg
    11281 gaatctgggt ccagagcggc attagttagg aaattaaccg
//
LOCUS       DASCNS010000081         3399 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000667174, whole genome shotgun sequence.
ACCESSION   DASCNS010000081 DASCNS010000000
VERSION     DASCNS010000081.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000667174"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(113..811)
                     /locus_tag="QF838_03090"
     CDS             complement(113..811)
                     /locus_tag="QF838_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59711.1"
                     /translation="MPLFTDQITQLESPIDVMYLMHKVYMKHSDFTENLALEAQEGGD
                     LTVFKANLDAWLKHLLYHAQTEDEYMTGPLKDKILQDGREPLKANEVEHDELRQFGGS
                     VLELLDQASAENVLASEEEEHAKLMEKVDQVEEAAGDAIAERFVKPRTRRHLYRSVME
                     LRVSEFDHFENEEAFVLPIVKEQMSSSEELECSRRLLIDDDSDQPRWIIDFIIDQLEG
                     VEKQLVMDLDKQFQ"
     gene            complement(907..1473)
                     /locus_tag="QF838_03095"
     CDS             complement(907..1473)
                     /locus_tag="QF838_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012720.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HJO59712.1"
                     /translation="MSTQSEVHTTSHDEFSMKRVGLWIFILSETFLFGALISTRFYLQ
                     GVERPDHLNQPLGLAISLVLLMSSLAAFRGEVAVAHNNQQKFRKNIIATIVLASIFLI
                     GVGWEWYEAFHYFPPSSAFGTIFFTMTGIHAFHVITGIIALIVIVRMGSDGRFNSDSH
                     WGVEGVVKYWHFVDVAWVFIYPTLYLVN"
     gene            complement(1502..1711)
                     /locus_tag="QF838_03100"
     CDS             complement(1502..1711)
                     /locus_tag="QF838_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59713.1"
                     /translation="MDDKEITRKFRKGLYVFIALLIVEVAEYIIGVRVTSGNFIFMVL
                     LAAPGSWLIVHYFMHIKELWIPPEE"
     gene            complement(1745..>3399)
                     /locus_tag="QF838_03105"
     CDS             complement(1745..>3399)
                     /locus_tag="QF838_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HJO59714.1"
                     /translation="LLGVGVWNSWVREWIGIKPSYFEAKNWQRYFGFSMDHKVIGVQY
                     LVTFVILFLLGGFFALLMRIELADSGEGLLGPNYYNTIMSLHGIIMVAVAVAAIIGGL
                     SNYVLPLMIGAEDVAFPRINALSFWIIPPVAILLILTPLFGGFDSGWTAYPPLSVTNA
                     DGQLLFLFAFLTFGLSSILGGLNFLTTTICLRAPGMTWGRLPIFIWSIVAASIISVTA
                     TQVVAFGLLMVILDRVAGMVFFDAGLGGKPLLYEHVFWFYSHPAVYIMILPAFGALLE
                     MVSHFSRKPLFGYKLVVASFLSITALSFLVWAHHLFTSGMEEFLHIPFMALTELISIP
                     TGIVFLSALGTIWRGKLWLVTPMLFVVGTVFNFLIGGLTGIFLADVATDIHLQDTYFV
                     VAHFHYTMMGGEIFMLFGAIYYWFPKMTGRLYNETLGRLHFTTMFLTYNALFITMFIV
                     GTDGMNRRVWTYPENLENGNILIGIFALLLGLSFIPFVYNMVVSWAKGAVSGNNPWGA
                     TTLEWATDSPPPEENFTHQPKVDEGPYTYGQPGRKETQATSI"
BASE COUNT         1022 a          781 c          653 g          943 t
ORIGIN      
        1 gggtttatgc tgatgtgcgc tttgtttgcc ttaacgtttc tcgaattagg ataaactcac
       61 tcaggaatag gatcgattat tggctttcta gaccctgagt ctatattgct gtttactgga
      121 attgcttatc taggtccatg actaattgct tttccactcc ctccagctga tcgataataa
      181 aatcaattat ccatcgtggc tggtcagaat catcgtcgat aaggagtctt cgagaacatt
      241 ctagctcttc actgctactc atttgctctt taacgattgg aagaacgaaa gcttcttcgt
      301 tttcgaaatg atcgaattcg gacactctta attccataac acttcggtat aaatgtcgtc
      361 gtgttctggg ctttacgaaa cgctctgcta tcgcatctcc cgcggcctct tcaacctggt
      421 cgactttttc cattaatttg gcgtgctctt cttcttcact ggctaacacg ttttctgctg
      481 aagcctggtc aagaagctcc agaacagatc ctccaaattg tcgaagttca tcatgttcca
      541 cctcattggc cttaagaggt tcgcgaccat cctgcaatat cttatcctta agtggccctg
      601 tcatatattc atcttctgtc tgggcatggt atagaagatg tttgagccac gcatcgagat
      661 tagctttaaa cacagtgagg tctccacctt cttgagcctc caaagccaga ttctccgtaa
      721 aatcagagtg tttcatgtag actttgtgca ttaaatacat tacatcaatc ggactttcca
      781 attgagtaat ctgatcagta aataacggca tggtgtcctc ctatttccag ctgaatagac
      841 gcaaactata agtttttgaa actccttttg tttccgatga ccttaatcaa aaaatctcgc
      901 caatatttag ttcacaaggt agagggtagg gtagatgaat acccatgcaa catcgacgaa
      961 gtgccaatat ttaaccaccc cctcaacccc ccaatgagaa tcgctattga atcttccatc
     1021 cgatcccatt cttacaatta cgatcagcgc aattattcca gtaataacat gaaaagcatg
     1081 tatacctgtc attgtgaaga atattgttcc aaacgcactt gaaggcggaa aatagtgaaa
     1141 tgcttcgtac cattcccagc ctactccaat cagaaaaata cttgccaaaa cgatagtggc
     1201 tataatattt tttcgaaatt tttgctggtt gttatgggca actgcaactt cacccctaaa
     1261 tgctgccaaa ctactcatca aaagaacaag tgatatggcc aatcccaggg gttggtttag
     1321 atggtctggt ctttcgactc cttggagata aaatcgggtg gaaattaagg caccaaataa
     1381 gaacgtttcc gataatataa atatccataa cccaacacgt ttcattgaaa actcatcgtg
     1441 gctggttgtg tggacttctg actgtgttga cactttggta agtactccct tactacttga
     1501 tctattcttc tggagggatc cagagttctt ttatgtgcat gaagtaatga acaattagcc
     1561 aggatcctgg agcggctaac agtaccatga agataaagtt tccactggtt actctaaccc
     1621 cgataatata ctcagctact tctacaataa gtaacgctat aaatacatat aaaccctttc
     1681 ggaattttct ggtgatctct ttatcgtcca cttatcaatc cttataattg caaatttctc
     1741 aacactagat agaagttgct tgagtttctt ttctcccagg ttgcccgtat gtgtaagggc
     1801 cttcatctac ctttggctga tgggtgaaat tctcttctgg gggaggagaa tctgtggccc
     1861 attccaatgt cgtggcaccc caaggattat taccggagac agccccctta gcccaggata
     1921 cgaccatgtt gtatacaaat gggataaaag ataatcctag gagtagggca aaaatcccta
     1981 ttaggatatt gccattttcc aagttttctg gataagtcca aacccttcta ttcattccgt
     2041 ctgttccaac tataaacata gttataaata aagcgttgta agttaggaac atcgtggtga
     2101 aatgaagtcg gcccaatgtt tcgttgtata acctaccagt catttttggg aaccaataat
     2161 agattgcgcc gaacagcata aaaatttcgc ctcccatcat tgtgtaatga aagtgagcaa
     2221 cgacgaaata tgtgtcctga agatgtatat cagtggcaac atctgcgagg aatattcccg
     2281 ttaaccctcc gatgagaaaa ttaaacacag tccccactac aaaaagcatg ggtgtaacta
     2341 accacaattt gcctctccat atggtgccta aggcagagag aaagactatt ccggttggga
     2401 tggatatcaa ttcagtaaga gccatgaatg gaatatgcaa aaattcttcc attccacttg
     2461 taaataaatg atgagcccaa actaggaaac tcaaggccgt gatagacagg aaagacgcga
     2521 ccactaactt gtatccaaac aaaggctttc gagagaaatg gcttaccatc tccaatagag
     2581 cgccaaatgc tggcaagatc ataatgtata cagctggatg ggaatagaac caaaacacat
     2641 gctcatacaa aagaggtttt ccacctagtc cagcgtcaaa aaacaccatg cctgcaaccc
     2701 tatctaatat gaccataagc aatccaaaag ctaccacttg agtagctgtt acactgatga
     2761 ttgaagcagc cactattgac catatgaaaa taggcaacct accccaagtc attcccggag
     2821 ctcgtaagca aatggttgta gtcaagaaat tgagccctcc caatatggaa gaaagcccga
     2881 aggtcaaaaa tgcaaagaga aaaagcaact gtccgtccgc gttggtgaca cttaatggcg
     2941 gatacgctgt ccaacctgag tcaaaacctc cgaaaagagg tgtcaaaatt agcaagattg
     3001 cgactggagg aataatccag aagcttagtg cgttaatcct aggaaaggcc acatcttctg
     3061 ccccaatcat aaggggtaag acgtagtttg aaagccctcc gatgattgca gccacagcca
     3121 cagccaccat aataatgccg tgaaggctca taatggtgtt gtaataatta ggtccgagaa
     3181 gcccttctcc tgaatcagct agttctatac gcattaataa agcaaagaaa cctccaagta
     3241 gaaacaagat tacgaaagtg acaagatact ggactccgat caccttgtgg tccatgctaa
     3301 agccgaagta acgctgccaa ttttttgcct cgaaataact cggctttatc cctatccact
     3361 ctcgtaccca gctgttccag actcctactc ccaataacc
//
LOCUS       DASCNS010000082         2790 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000671591, whole genome shotgun sequence.
ACCESSION   DASCNS010000082 DASCNS010000000
VERSION     DASCNS010000082.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000671591"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="QF838_03110"
     CDS             complement(<1..862)
                     /locus_tag="QF838_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HJO59715.1"
                     /translation="MKSLILKEPGRSPDIRVLDLPEPILTADGIIIKVAAAGLCHHDI
                     SVMDGTLRRGTKPDVILGHEISGTVVDIGNEIREFRLGDQVVTTLTSSCGECGTCVNG
                     DDYMCGFGLGYGHGIDGGFAEYILVREKNLVNVGDMDLVQSALLSCPIGVSIKAIFDR
                     ASLETDDSCVVFGTGGGLGIHALQVAQAYTSNVIGFTSSPSKLEEIGKYEVSPTFLLD
                     EGIDPTDLVMALTDDKGADVIFNSVGSKFLKSSVSSLKHGGRVLMMGELLGGNASLNT
                     SDLLFKNGSII"
     gene            complement(887..1762)
                     /locus_tag="QF838_03115"
     CDS             complement(887..1762)
                     /locus_tag="QF838_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HJO59716.1"
                     /translation="MRINTVAIMSPGDMGHAVGKVLSESGLDVITCTEGRSLRTRNLA
                     KVAGFRQVASLSDLVSEADLILSIMVPSRAMDFATEIAPHMESTASQTFYADCNAVSP
                     QTSQNISEVINNSGGVFIDGGIIGGAPTKGDIPRFYVSGPDAAIVMELDGKGITVKTV
                     GDQIGQASGIKMCYAALTKGTHTLHVALLTAANKMGLTEDLRNEFEFSQKAHLNAMEK
                     GISRLPANAHRWIGEMEEIASTFEHLGVTPNFHKGAADVYKMLNATPFAEETPETIDN
                     DRTALETIEAVSNLD"
     gene            1903..2754
                     /locus_tag="QF838_03120"
     CDS             1903..2754
                     /locus_tag="QF838_03120"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HJO59717.1"
                     /translation="MKLGAHVSTSGGLSRSIDRAQAIGAEAIQIFASSPRAWKFNFPK
                     EEEIALFKEKSLETGIGPCYIHGSYLVNIGGDESQLEKSIESLTNNMITAGNIGAEGV
                     IFHGGSHKGRGFNTVLDQAVKCLIAVLEESPNDTWLCIENSAGMGSHIGSSFDEIGAI
                     IKAVNHPNLKVCLDTEHCFAAGYNIASGDDIDSIMEEFDSKIGLERLVAVHANDAKVE
                     FGSGVDRHENIGEGYIGEEGFEVILANPAFSQVPFFLEVPGFDGEGPDKENINRLKSI
                     RNKVLEE"
BASE COUNT          842 a          641 c          535 g          772 t
ORIGIN      
        1 caataatgct tccattttta aacagtagat cggaagtgtt aagtgaagca tttcctccta
       61 acaactctcc catcatcaaa accctaccac catgctttaa agagcttact gaacttttca
      121 aaaattttga cccaacagaa ttaaaaataa catcagcccc tttatcatca gtgagggcca
      181 ttactaaatc agtcggatct atgccctcat caagtaaaaa agttggggaa acttcatatt
      241 taccaatctc ttccaattta cttggggagg acgtgaagcc aataacatta gatgtataag
      301 cttgtgccac ctgaagggca tgaataccta atccacctcc agtcccaaaa actacgcatg
      361 agtcatctgt ttccaaagaa gctctatcaa aaatagcttt tatagaaacc ccgatagggc
      421 aggataagag cgcactctga accaaatcca tatcacccac gtttaccaag tttttttctc
      481 ggaccaaaat atattcagcg aaccccccgt caataccgtg accataccct agcccaaatc
      541 cgcacatata atcatcccca tttacacatg ttccgcattc tccacatgag gaggtaagtg
      601 tggtcactac ctgatctcct aaacgaaatt ccctaatttc attaccgata tccactactg
      661 tgccagatat ttcatgaccc aatatgacat caggtttagt tcctcttctt agagtaccat
      721 ccatcacaga tatgtcatgg tgacacagac ctgcagcggc aacttttata atgattccgt
      781 cggcagttaa aattggttca ggaagatcaa gaacccgtat atcagggctc cgaccaggtt
      841 cttttagaat tagacttttc atagattttt atcgttgatc tctttattag tctaaattag
      901 atacagcctc tatagtttcc aaggcagttc tgtcattatc gatggtttcc ggagtttcct
      961 ctgcgaaggg agtggcgttg agcattttat agacgtcagc tgcgccctta tgaaaatttg
     1021 gagtaacacc taaatgttca aaagtggatg cgatctcctc catttctcct atccatctat
     1081 gtgcattggc tggcagtctt gaaattcctt tctccattgc attgaggtgt gctttttgac
     1141 tgaattcaaa ctcatttcta agatcctcgg ttaaacccat tttattagca gcggtaagca
     1201 aagctacatg gagtgtatgg gtccctttgg tcaaagccgc ataacacatt tttatacccg
     1261 atgcttgtcc aatctgatct cctactgtct tgacagttat acctttacca tccaattcca
     1321 tcacaattgc tgcatcagga ccagaaacat aaaatcttgg gatatccccc ttcgttgggg
     1381 ctcctccgat tattcctccg tcgatgaata cccctccaga attatttatc acctctgaga
     1441 tattttgcga agtttgcgga gatactgcat tgcaatcagc ataaaaggtt tgactcgccg
     1501 tggattccat atgtggagcg atttctgtcg caaaatccat cgcccttgat ggaaccataa
     1561 tggacaatat tagatcagct tcactcacca aatctgacaa actagcaact tgtctgaacc
     1621 ccgcaacttt ggccaaatta cgggttctaa gacttcgccc ttcagtgcag gtaatgacat
     1681 ccaagccact ttcagataaa accttaccaa ctgcatgacc catatcgcca ggactcataa
     1741 tcgctacagt attgattcgc acaggcatct ctccaatggt gttcttatat tcataaaacc
     1801 ttaattatag gcaatactta ctatcgatca tagttctaat gagcttggct cacatcccca
     1861 tgataaactg gaataaaaga gcgacacaaa caggaaccca acatgaaatt aggtgcacac
     1921 gtatccacct ctggtggact aagccgatca atcgataggg ctcaggcaat tggtgcagaa
     1981 gctatacaaa tatttgcatc atctcctcgg gcctggaaat tcaatttccc taaggaagag
     2041 gaaattgcac tttttaaaga aaaaagtttg gaaactggaa taggaccctg ctacatacat
     2101 ggtagttact tggtaaacat aggaggagat gaaagccaac tcgaaaaatc aatcgaatcc
     2161 ctgacaaata acatgattac tgccggaaat attggagcag aaggagttat tttccatgga
     2221 ggaagccata agggtagagg cttcaacacc gtactagacc aagcagtaaa atgtctcatt
     2281 gcagttctag aagaatctcc aaatgacaca tggttatgca tcgaaaatag cgcaggcatg
     2341 ggatctcata taggttcttc ctttgatgaa atcggggcca ttataaaagc agtgaatcat
     2401 cctaatctta aagtgtgcct agacacggaa cactgttttg cagctgggta caacatagcc
     2461 tctggggacg atatcgattc gattatggag gaatttgact ctaaaattgg gttggagcga
     2521 ctggtcgctg tgcatgctaa tgacgctaaa gtcgaattcg ggtctggagt agatagacat
     2581 gaaaatattg gggaaggcta catcggtgaa gaagggtttg aagtcatact cgcgaacccg
     2641 gcattttctc aggtaccatt tttcctagaa gttcccggct ttgatggaga gggccccgat
     2701 aaagagaata tcaatcggtt gaaatcaata agaaacaaag tactagaaga gtaacgggat
     2761 gtcctccgac ctttccagat ctgaacctac
//
LOCUS       DASCNS010000083         1862 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000680334, whole genome shotgun sequence.
ACCESSION   DASCNS010000083 DASCNS010000000
VERSION     DASCNS010000083.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000680334"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="QF838_03125"
     CDS             complement(<1..910)
                     /locus_tag="QF838_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO59718.1"
                     /translation="MKITEIKTFRMSATPHKSDTNNWLFIKIYTDAGIYGVGEGSLQY
                     KDLGLMAEIEDFSKFLIGKDPFAIEYTWTSLYRRVTWTGGAVTMSAISAIDLALWDLK
                     GKALGVPVYELLGGKVRDAVPVYANGWFEDAVTPEDHAEMAIKTVESGYPCLKFYPFK
                     GQYVTTPERIKTGVELVAAVRNAVGPDIQIGIDVRARLDFWSALQVAEELIPYKVSWI
                     EEPIQFDNPKLMGEFARKSRIPVSTGEQLYNRWDFQPLLDEKSISIIQPDICHAGGIS
                     ELRKIASAAETQYVNVAPHNSNGPIST"
     gene            1010..>1862
                     /gene="thiL"
                     /locus_tag="QF838_03130"
     CDS             1010..>1862
                     /gene="thiL"
                     /locus_tag="QF838_03130"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HJO59719.1"
                     /translation="MTKVSEISEFDLISRLETVLENNVTPNPDSKIELSIGDDAAVIG
                     NLDNLQVVTTDAMVDKVHFVIDEVDMRDLGWKSLAVNYSDIASMGCKPIYSVVTLGIR
                     ADILVEQLESLYLGFSDLLNIYGGELVGGDIVKSDTFFISVTVVGSTDKPETMKRKTA
                     VPGDVIAVTGALGCSNAGLRLLLNGELKKPSHFYDAHYLPKPRITEGINLLDMGIKTG
                     MDISDGLINDLKKICVASNVNATINIDNIPVHEDLKASFPDNFTEMTVSGGEDYELLV
                     KAHESLIK"
BASE COUNT          558 a          390 c          370 g          544 t
ORIGIN      
        1 ctgtagatat tggtccattt gaattgtggg gggctacatt tacatattgc gtctccgctg
       61 ctgaggctat ttttcttaac tctgagattc cacctgcatg gcatatatca ggctgaatta
      121 tcgaaatcga tttttcgtcc aataatggct gaaaatccca tcggttgtac aactgctccc
      181 ctgtagacac agggattcga gatttccttg caaattctcc catcaacttt ggattatcaa
      241 actgtatagg ttcctcaatc caagaaacct tgtatggaat aagttcttca gcaacttgca
      301 gcgcactcca aaaatccaaa cgagctctca catctattcc gatctgaatg tccggaccaa
      361 cagcatttcg tacggcggct actaattcaa caccagtttt gattctttcg ggagttgtta
      421 cgtattgacc tttaaacgga taaaacttaa ggcagggata tcccgattcc accgtcttta
      481 ttgccatttc tgcgtggtct tctggagtaa ctgcatcctc aaaccaccca tttgcgtaga
      541 ctggaactgc atcccttact tttccaccaa gcaattcata gacaggaacc ccgagagcct
      601 tacccttcaa gtcccatagg gctagatcta tagcactgat agcactcata gtcacagctc
      661 caccagtcca tgtgacccgt ctgtacagtg aggtccaggt atattcaatt gcaaaaggat
      721 ctttcccaat caaaaatttt gaaaaatctt ctatctctgc cattaaacct agatctttgt
      781 attgaaggct gccctccccg acaccataaa ttcctgcgtc ggtatatatt tttataaata
      841 accaattatt ggtatctgat ttatgtggcg ttgcggacat gcgaaatgtt ttaatttctg
      901 taattttcaa ttaccgtact ccaataaagt aaatataata tgactaaaag cttttatacc
      961 acttcgtagg ctctaagtca tatgtgatag agaacaaagt agcatttaga tgacaaaagt
     1021 ttcagaaata agtgaatttg atcttatatc taggctggaa accgtcctag aaaataatgt
     1081 cacgcccaat ccggacagta agatagaact ttcaataggt gatgacgctg ctgttatagg
     1141 taacttagat aaccttcaag tggtgacaac cgatgccatg gtggataagg ttcatttcgt
     1201 cattgatgaa gtcgatatgc gagatctggg ctggaagtca ctggctgtga attacagtga
     1261 tatagcctcc atgggatgta aaccgatata ttctgtcgtc actttaggaa taagagcgga
     1321 catattagtg gagcaattag aaagtcttta tttaggtttc tcagatcttt tgaatatcta
     1381 cgggggagaa ctggtcggtg gagacatagt aaaatcggac acatttttca tttccgtgac
     1441 agtggtggga tccacagata aacccgagac catgaagagg aaaacagcgg ttcccgggga
     1501 tgttattgct gttaccgggg cccttggttg ctccaatgct ggactaagac ttcttttaaa
     1561 tggtgagctc aaaaaaccat cgcatttcta cgatgcgcat tatctgccaa aaccacgaat
     1621 taccgaaggt atcaatcttt tagatatggg aataaaaaca ggaatggaca ttagcgacgg
     1681 attaattaac gatcttaaaa aaatatgtgt tgctagtaac gtcaatgcga ctataaatat
     1741 agacaatatc cctgtacacg aagacctaaa agcatcattt cctgataatt ttacagaaat
     1801 gacagtaagc ggtggtgaag attacgaatt gctggtcaaa gcacacgaat ctctcatcaa
     1861 at
//
LOCUS       DASCNS010000084         1058 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000683634, whole genome shotgun sequence.
ACCESSION   DASCNS010000084 DASCNS010000000
VERSION     DASCNS010000084.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000683634"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..317)
                     /locus_tag="QF838_03135"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="QF838_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372994.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          293 a          224 c          241 g          300 t
ORIGIN      
        1 ggccttagct atcccatcca tctgccgttc ggaacgcagt tcggtctcga actgagaaat
       61 agccgacaac atatggaaca gaaaacggcc ggtagcatca ctagtatcaa tattctgatc
      121 caagacctgc agatcacctg cgggtcttgg agtttgtcga ctatttatta ttccagacaa
      181 gcctttaatc tgggacgttg atctgaagtc ccactcttct gttcctcaaa taccttgtgg
      241 cagtgtttca atttgtcgcg ttggacctcc aggctttgtc ccactgaact gacccgggca
      301 taacccacca atgccatctt atcctcctca agtatgtttt atgtggtctt agatgatttg
      361 ataatcctat tataaaacat ggcctgagac acatcaagac ccaggtctca gttagcgtgt
      421 gtagttattg agatgaaaca tgttggtcta ttagcttgtt ttggcttctg gttctatttg
      481 gcctacacgg ttttaccagt aaaacttcga tagcctaagt aaaatagaga atagtaaatt
      541 tagttcgaca catggttcct caacatcaat cctcaataga ataaactcgt gttgatgtga
      601 tttgggatta tcgtaaaagt aggtgtgccg gtgactttca gctaagtata accatacggc
      661 tgatgagctt ttcggtttgg cgtctgatcc ctaccagctc gacaatctgg tcaaccactc
      721 ctaacacatg ggcaaactgg caaagataag gaccgactta aaatagtgga tgtagcaaaa
      781 gcgagattct ggcctagctt tgattccgtt tggaggaaag aaggaacacg aagactttgc
      841 gtttcggatc aattttgact agggatagaa aatagtctaa aatgggttat tcaggtgagg
      901 tgaatcgatg aagatcataa ctaacgattt aggtgcagat aggatggatc gggtgcagca
      961 aatcgcccct caagcgaatt ttgtgattac gaccgactat cagcatacct tacgcgagat
     1021 tgcagatgca gatgtgcttt gggggcaccg gataacac
//
LOCUS       DASCNS010000085         2670 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000685443, whole genome shotgun sequence.
ACCESSION   DASCNS010000085 DASCNS010000000
VERSION     DASCNS010000085.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000685443"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="QF838_03140"
     CDS             complement(<1..857)
                     /locus_tag="QF838_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013528.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HJO59720.1"
                     /translation="MKYEKWIVLPKATDSEIAELGLGGVESTLLVHRGIYTEDDLDEF
                     LSPTLSSVHDPFLLPDMSNSVDRLIQAVDSNETVGVFGDFDTDGISGTALLTRGLENL
                     GLKVFPYVPHRVDEGHGISPQSLEFFSSQGVTLLITVDCGVTSIDEIQTALDMGIETI
                     VTDHHTALDGMPHAIGIVNPSLPNSSYPFKYLTGVGTAFKVMQALYLRKKIELPDELY
                     VYVTLGTISDVGLMRDENRYLVSKGMDVLRKSELVSLDAWIKVANASKKNLSTQDMSF
                     GIIPRINVAG"
     gene            complement(878..2116)
                     /gene="fabF"
                     /locus_tag="QF838_03145"
     CDS             complement(878..2116)
                     /gene="fabF"
                     /locus_tag="QF838_03145"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HJO59721.1"
                     /translation="MVERVFVTGVGSISPVGLSIEETWSNITKGVSGVDTISSFDPEG
                     YETTFAAEATSFDPEAYVDRKQARRMDRFVQLAAAASLEAFDNSGISINNGNADRVSV
                     MVASGIGGIITLSDQIGVLNGRGPTRVSPFLVPMMLPDMASGQVSMLLGAKGANYCTV
                     SACASGADSIGMAFEAIRRGDVDAAVTGGAEAAICPVGVAGFNACQALSKRNDDPQAA
                     SRPFDATRDGFVLGEGAGVLVLESQTSMDSRGAEPIAEMIGYGSTADAYHITQPGPEG
                     EGGARAMNMALSKAGISPENIDYINAHGTSTPLNDKFETMAMKSVFGEHAYDMPISST
                     KSMTGHLLGASGALEAAITVMAVKNQIAPPTINLRNSDPDCDLDYTPDSPRQTTITTA
                     MSNSFGFGGHNASLIFQNPS"
     gene            complement(2239..>2670)
                     /locus_tag="QF838_03150"
     CDS             complement(2239..>2670)
                     /locus_tag="QF838_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59722.1"
                     /translation="PEATTFMEKSRIPTISDSSALSVSMLWIDAADKDHLIFTALNDI
                     QSHFLAENEVDDVETVRGVEPFMSGDDLISSFYCLWTLVWDGFRGISVKLSTTSSGYS
                     PVMSIKCHSEGSEIVFSAVDDDKKFLKCLGDCFFAETSVIK"
BASE COUNT          773 a          656 c          510 g          731 t
ORIGIN      
        1 cccgccacat taatcctagg aatgatgcca aatgacatgt cctgagtgga taaatttttt
       61 tttgaagcat tggctacctt tatccatgca tcaagactaa ccaactctga ttttcttaaa
      121 acatccatac ctttactgac cagataacga ttctcatccc gcataaggcc aacatctgaa
      181 atagttccca aggtgacata gacatataat tcatcaggta attcgatttt ttttctcagg
      241 tacagagctt gcattacttt aaatgcggta ccaacacctg tgaggtattt gaaggggtag
      301 gatgaatttg gaagagaagg gttcactatc ccgatagcgt gaggcatccc atcaagagct
      361 gtatgatgat ctgtcactat cgtctcaata cccatatcca aggcggtttg aatctcgtca
      421 atagatgtga ccccgcaatc aacagttatc aatagggtca caccctgaga agaaaaaaat
      481 tctagtgact gagggctaat cccgtgacct tcatcaaccc tatgcggaac gtatgggaaa
      541 acctttaaac ctaaattttc cagaccacgt gttaagagcg cagtccctga gataccatct
      601 gtgtcaaaat ctccaaatac tcctacagtc tcgtttgagt ctacagcttg tatcaaccta
      661 tccacagaat tagacatatc aggcaagaga aaaggatcgt gaacggaaga gagtgtaggt
      721 gaaagaaatt cgtctaaatc atcctctgta tatatccctc tatggaccaa cagggttgat
      781 tccacccccc ctagacctaa ttcagcgatt tctgaatcgg tggcttttgg taaaactatc
      841 catttctcat atttcaatgt cgataaacct tacaaaatca acttgggttt tgaaagatta
      901 aagacgcatt atgcccgcca aaaccaaaag agttactcat tgcagtcgta atggtagtct
      961 gccttggact atcaggcgtg taatctaggt cacaatccgg atcgctattt cttagattta
     1021 tagttggtgg tgctatctgg tttttaaccg ccataactgt aatagccgct tctaaggctc
     1081 cactggcccc caataggtga cctgtcatag atttggtgga acttataggc atatcgtatg
     1141 catgctcccc aaagactgac ttcatcgcca ttgtctcaaa tttatcgttt agaggtgtgg
     1201 aagtaccatg ggcattgata tagtcaatat tttctggact aataccagcc ttactgagag
     1261 ccatattcat ggctctcgcc ccaccctctc cttccggacc aggttgggtt atatggtaag
     1321 catctgcagt agaaccatat ccgatcattt ccgctattgg ctctgcaccg cgagagtcca
     1381 tggaggtttg gctttctagt acaagcacgc cggcgccctc accaagaaca aatccatccc
     1441 gagtggcatc aaaaggcctt gaggctgcct gggggtcatc atttctttta gataacgcct
     1501 ggcaggcgtt aaaacccgcc acacccacag gacaaattgc cgcttcagct cccccagtga
     1561 cagcagcatc tacatcacct cgccgaatcg cttcaaatgc cataccaatt gagtctgctc
     1621 cactcgcaca ggcagaaacg gtacagtaat tggctccttt tgcccctaat agcatcgata
     1681 cctgcccgga ggccatatca ggcagcatca taggaacaag gaaagggctt accctggttg
     1741 gccctcttcc attcaaaaca ccaatttgat ctgaaagcgt aatgattcct ccgataccac
     1801 ttgctaccat tactgacact ctgtcggcgt ttccattatt tattgaaata ccactgttgt
     1861 cgaaagcctc taatgatgct gccgcggcta attgaacaaa ccggtccatt ctcctagctt
     1921 gctttcggtc gacataggct tccgggtcaa aactggtggc ttctgcagca aaagtagttt
     1981 catatccttc cggatcaaag gaggaaatag tatctacccc tgacacgcct ttagttatat
     2041 tggaccaagt ttcctctatc gataaaccaa ctgggctaat agaaccaacc ccagtgacaa
     2101 aaactctttc aaccattaaa ttctcccgat attaaatgtg aaaagtctct tggagaaaac
     2161 tggactatat agagcacttt tcttatgctg ttcactcggg attataacag tcgactcagg
     2221 cgtgacatat aggtaagact acttaattac gctggtttcg gcaaaaaaac aatcacctaa
     2281 acacttaagg aattttttgt catcgtctac tgccgaaaaa acaatttcgg aaccttctga
     2341 gtggcattta attgacatta ccggcgaata ccctgaagac gtggtactca atttgaccga
     2401 aatcccccta aatccatccc agactaaagt ccaaagacag taaaaggaac taatcaaatc
     2461 atctccagac ataaagggtt caacacccct aacggtttct acatcatcaa cttcattttc
     2521 agccaaaaaa tgactttgta tatcatttag agctgtaaaa attaaatgat ctttgtcagc
     2581 cgcatcgatc cacagcattg aaacactcaa tgctgagcta tcggaaattg tcggtatcct
     2641 gcttttttcc ataaatgtgg tcgcctccgg
//
LOCUS       DASCNS010000086         5875 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000696123, whole genome shotgun sequence.
ACCESSION   DASCNS010000086 DASCNS010000000
VERSION     DASCNS010000086.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000696123"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="QF838_03155"
     CDS             <1..1625
                     /locus_tag="QF838_03155"
                     /inference="COORDINATES: protein motif:HMM:NF025330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HJO59723.1"
                     /translation="ATLTEIYDPANDSWEKSSSLLTPRWGHTATLLDDGRVLVAGGTN
                     NSSPALESEIFDPDTETWVSAGQMKYERTGHTASLLSDGRVLIAGGFTYVPISYSEIY
                     DVEKGWVKTENLMGPRHMHSAVPLNDGQVLVIGGKAGLFTNLTRVELYDSDSGSWSIK
                     GKMPVALWGHTAIPLDCENVLIVGGKRNDGSSFNLAYLFNATTGGYQTTGKMNVPRFA
                     HSATKISDGSVVVTGGYINPSFQSDSVEILKPNKSGSYEWVPAMPVPVPLERHSAIHI
                     GERRLFITGGWNGSSATSDTWIFDLDNNLWDVGPPMRDARWGHSMVWLDDKTVLISGG
                     VGDDGLPLSSVEKYDSVNSSMESLSSMQEARSGHGLLKDKNGNVYAIGGYGSEGISLP
                     SSEKYHVSFDKWNKLPDMSEARSHHSAIILKDGNILITGGTDDYNTPSGTAEIFDMKS
                     GQWKSAGSMREARMGHVSYQFDSKNGFNCPDLATHGEVFIMGGYGLSVMDSVEVYRPG
                     GGGWSAFESIVRAPTKDNFTRFVRVISQNWNR"
     gene            1613..5287
                     /locus_tag="QF838_03160"
     CDS             1613..5287
                     /locus_tag="QF838_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HJO59724.1"
                     /translation="MESLNFFLLVVRRSFNDRRLILSILSGMLVATTLMSSAPMYLAA
                     LDQQGVRQMVDLTTKNKSENYLALDMFVPSMALSKERIFHSDELIQQAVNLHFLEAVV
                     GSNRYLKTDGFSFYRSSDSAIRDLEKGPIRSLEGYFHSFQSMKKEVDYIVGSPPTSDF
                     QYHPDGPLLEVSLSTDLAEKLSIEYGDVLLGLPYPDSRIKLGVRVSGIFEALDPYNPI
                     WEDDIDRFLIPVIPKDEGSEVPDKWWEDQKYYLALFISEESMMNSIPLAYPGAMVEAR
                     WNQQINNLNLREWTRAEMSSSVDGLDGLLTKQIPGFQLRSGIILMLKEFRTQSFFSSI
                     PLLLLFAVIGSSLLYFVFMISSYLIPAREADISLIKTRGASRFTVFRQYGLEGFLLFL
                     VSTIFAPILAFCLVSLSGILPYFHSISDGKLLPTTLSWTPFILSFGFGVLCFMAFLLP
                     PILRTSGRIVTRRRYLGRPESVTFIQKYYLDFAFLFICSVLLYEIKARGQLSSGGLER
                     GSGINEALLFAPILLLFSVAILSFRIFPILVKFMTGESSSLILLMGTLSVIFLSLNIC
                     YDLLTNVDKLGFLLPAFVFLLLLIGLLACFKASNKSSKYISFIFILGASLIYGVYTPE
                     GDVILFSLTKWVLAFTSLLTGIFFLLSSFKKCYPPWISLMLWNMSRNPLQYTWLVVLL
                     VLSSGVAVLSAALGSTLDHSYRDKIAYDVGSDARIVLSSLGSVGRMHSLPSLSNFLGN
                     LPGVQKFSKAKRGIGNIGQGADSFTFNYLAFETEKITLWGREDFSAQSTDIVFGDIPS
                     EVMTPAIEIPIGATEIGINVKPDSNYPLVSLWLILRDANLKYKVITLGTADPSKWSKN
                     KAVIPDSLVEPLQLVSVQISEPGFGASGTPGKMFFDDLFAILNNEEVILEDFEDYSEW
                     VIIPTSSGPGDSISIEEEAAVTGRFGFSFEFGKEMNNGIRGLFWGTYGSYLPVVVSQT
                     FVDNSGFGFGDYLFLDISQTLVIGKITGVVDFFPTLNPGTGGFLLADLDAVMYLSSLT
                     NPHSMSSANEIFLELNESVDDTIAAISDEVGKGGQVHATKRLLEKLDNDPLISAGWKL
                     MSSISITVALYIALLGYFIHMIFLSGQIKSRMGTLRSLGMTLSQSFRMIFVEQILLIL
                     LGVGLGTWTGVGMTRLMVSAIATTDNAVSLVPPALITIDFLSLLICAGTFFALFTFMV
                     IWVSLYFFRLNLGTLSRLED"
     gene            5288..>5875
                     /locus_tag="QF838_03165"
     CDS             5288..>5875
                     /locus_tag="QF838_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59725.1"
                     /translation="MMEIEGFAKIIRVWPLIVKRLVVNWRTLSTVIIGVVLACSIMSG
                     TVVFFDSLKEIALADVLKSLDKEEINILVQAEKGPTNVEEASILNERIHSFSEGLLSS
                     HVNEILHGGRTTTFFFSYQGQEQDAGKDNMRTFFAYLPNLQSHITITDGLFPGNVTGE
                     LLEDIPVVKVLVNVEDAAIFGLQIGDRISAVPYWDD"
BASE COUNT         1547 a         1068 c         1365 g         1895 t
ORIGIN      
        1 ctgcgacctt aactgagatt tatgatccgg ctaacgacag ttgggaaaaa tcatcctctt
       61 tgttaacccc tcgatggggg catactgcta ctttgcttga tgacggtaga gttctcgtgg
      121 ctggaggaac aaataattct tcgcctgctc tcgagtctga aatttttgat cctgacactg
      181 aaacctgggt gtcggcaggg caaatgaaat atgagagaac cggccatact gcttctttgc
      241 tttctgatgg aagggtttta atagctggtg gttttacgta cgtaccgatc agttattctg
      301 aaatttatga tgtggaaaaa gggtgggtga aaactgagaa cctaatgggc cctaggcaca
      361 tgcattctgc tgttcctctc aatgacgggc aagttttagt aattggtggc aaggcaggcc
      421 tgtttactaa tttaacgagg gtggagctgt acgattctga ctcagggtcc tggagtataa
      481 aagggaaaat gcctgttgcc ttatggggcc atacagcaat tcctttggat tgtgaaaatg
      541 ttctaatagt aggaggaaaa cgaaatgatg gtagttcttt taatttagca tatttgttta
      601 atgccaccac tggtgggtat caaacaacag ggaaaatgaa tgtacctaga tttgcccact
      661 cagctaccaa aatttccgac gggtccgtgg tagtaacagg gggatacatt aatccttcat
      721 ttcaatcaga ttctgttgag attctaaagc cgaacaaatc tggctcttat gagtgggtcc
      781 ctgctatgcc agtaccggta cctcttgaga ggcattcggc tattcatatt ggagaaagga
      841 ggttatttat caccggtggt tggaatggtt caagtgccac atcggatacg tggatttttg
      901 atttagacaa taatttatgg gatgttggcc ctccaatgag agacgcgaga tgggggcatt
      961 caatggtttg gcttgatgac aaaactgttt tgatctcggg tggcgtcggg gatgatgggt
     1021 taccgttgtc atcggttgaa aaatacgatt cggtgaattc ttcaatggaa tcgttgtcct
     1081 cgatgcaaga agcccgtagt ggccatgggc ttctcaaaga taaaaatggg aatgtttatg
     1141 cgatcggtgg atatggttct gagggcattt ctttgccgtc atccgaaaaa tatcatgtaa
     1201 gttttgataa atggaataaa ttacctgata tgtcagaagc aaggtcacac cattccgcca
     1261 taattcttaa ggacggaaat attctcatca ctgggggaac tgatgactat aacaccccat
     1321 caggtactgc agaaatattt gatatgaaat ctgggcagtg gaaatcggct gggagtatga
     1381 gggaagccag gatgggtcat gtgtcttatc agtttgatag taaaaatggg tttaactgcc
     1441 cagatcttgc tacccatggc gaggtgttta tcatgggtgg atatggtctg agtgtgatgg
     1501 actctgttga ggtttatagg ccaggtggag gaggttggtc tgcctttgaa tctatagttc
     1561 gagcccctac aaaggataat ttcaccaggt tcgtgagggt aatatcgcaa aattggaatc
     1621 gctaaacttt ttcttgctag ttgtaaggcg atcattcaat gaccgccgcc tgattttatc
     1681 catactatcc ggcatgcttg tagctaccac cttgatgtct tctgcaccaa tgtacttggc
     1741 tgccttggat caacaaggcg taagacagat ggtagatttg actaccaaaa ataagtctga
     1801 aaactatctg gcactggata tgttcgtccc ttcaatggcc ctgagcaagg aacgtatttt
     1861 tcattcagat gaattaatac agcaagccgt gaatttgcat tttttagaag cagttgttgg
     1921 ttcaaaccgg tatctaaaaa ccgatgggtt tagcttctac aggtcatcgg attcagcgat
     1981 aagggaccta gagaaaggcc ccataagatc actcgagggg tattttcaca gtttccagag
     2041 tatgaagaaa gaggttgatt acatcgtagg atcaccccca acttcggatt tccaatacca
     2101 tccagatggg cctttactag aggtatctct ctctacagat ttggcggaaa aactttcaat
     2161 tgaatacgga gatgttttac taggacttcc ctatcccgac agcaggatca aattgggggt
     2221 tagggtgagt ggaatttttg aggccttaga tccatataat cccatatggg aagacgatat
     2281 agatagattt ctaattccgg tgattcctaa agatgaaggc tctgaggtgc ctgataaatg
     2341 gtgggaggac caaaaatatt atttagctct ttttatatct gaagaatcta tgatgaattc
     2401 aattccgctg gcttacccgg gggctatggt tgaggcacgt tggaatcagc agatcaacaa
     2461 cctaaattta agagaatgga ctagggctga aatgtccagt tctgttgatg gtcttgacgg
     2521 attgcttact aaacaaatac cggggtttca acttagatct gggataattt tgatgttaaa
     2581 ggaatttaga acccaaagtt tcttttccag tattcctttg ctattacttt ttgctgtgat
     2641 aggttcttcc cttttatatt ttgtatttat gataagttca tacctgatac cggccagaga
     2701 agcagatatc tctctaataa agacccgagg cgcctctcgg ttcactgttt ttaggcaata
     2761 tgggctggaa gggtttttgt tgtttttggt ctcaaccatc ttcgctccga ttctcgcctt
     2821 ctgcctcgta tccttgtcgg gcatattgcc ttatttccac agtatttcag atggtaaatt
     2881 attgccaaca acgttgtctt ggactccttt tatactctca tttgggtttg gggtattgtg
     2941 tttcatggct tttttgttac ctcccattct acgaacttca ggacgaatag ttaccaggcg
     3001 acgttatttg ggcagacccg aatctgtgac ctttattcaa aagtattatc tcgattttgc
     3061 ctttctgttc atttgttctg ttctcttgta tgaaattaaa gccagaggac agctttcatc
     3121 aggaggattg gaaagaggtt caggtataaa tgaggctctg ttatttgctc cgattttgct
     3181 gctcttctct gtcgctattt tgtctttcag aatatttcct atcctcgtta aatttatgac
     3241 gggagaatct tcaagtctca tcttgttaat gggtactctt tcagtaatat ttttgtcatt
     3301 gaatatttgt tacgatctat taaccaatgt agataaattg ggattcttgt taccggcatt
     3361 tgttttccta ctacttttaa ttggtttgtt ggcctgtttt aaagcatcaa ataaatccag
     3421 taaatatatt tcttttattt tcatacttgg agcatcgtta atatacgggg tttatactcc
     3481 tgaaggggat gtcatactct tctccctcac aaaatgggtc ttggctttta cgagtttact
     3541 gacaggcatt ttctttttgt tgagtagctt caaaaaatgc tatccaccct ggatatctct
     3601 tatgctgtgg aatatgtcta gaaacccctt acagtacacg tggttagtcg ttcttttggt
     3661 attgtcatct ggtgtggcag ttttatcggc tgcattagga tcaactttag atcacagcta
     3721 tagagataaa atcgcctatg atgtggggtc ggacgcgagg atagttcttt cttcgcttgg
     3781 gtcggtgggt cgaatgcatt ccctacctag cctctctaat tttttgggaa atcttccagg
     3841 tgttcaaaaa ttttcaaaag cgaaaagagg tattggaaat attggccaag gggcagactc
     3901 tttcacattt aattatttag cttttgaaac agagaaaatt accttatggg gacgggaaga
     3961 tttttcggcg cagtcgactg atattgtgtt cggcgatatc ccaagtgaag taatgactcc
     4021 ggccatcgag ataccgatcg gtgccacaga aataggtata aatgttaaac ctgactccaa
     4081 ttacccactc gtatctttgt ggttgatact aagagatgcg aatcttaaat ataaggttat
     4141 aacactgggt acagctgatc cttctaagtg gtccaagaat aaagctgtga ttccagactc
     4201 tcttgtcgaa ccgctccaat tggtttcggt acaaataagt gagccaggct ttggtgcatc
     4261 gggtactccc ggaaagatgt tttttgacga tttgtttgct attttgaata atgaagaggt
     4321 catattagag gattttgagg attattctga atgggtgatc atccctacct ccagtgggcc
     4381 aggcgattct atctccattg aggaagaagc tgcagtaaca ggtagatttg gattttcatt
     4441 cgagtttggc aaagagatga acaatgggat tagaggttta ttttggggaa cctatggatc
     4501 atatttacct gtcgttgtaa gtcagacatt tgttgataat agcggatttg gatttggaga
     4561 ttatttattc ctggatatct cgcaaaccct agttattggc aaaataacgg gtgtagtaga
     4621 ttttttccca acgctaaatc ctggaaccgg tggatttttg ttggcagatc tggatgctgt
     4681 tatgtactta tcttcgctga ctaacccaca tagtatgagc tctgccaacg aaatttttct
     4741 ggagctaaat gaatctgtcg atgacacgat agctgctatt tccgacgaag ttgggaaggg
     4801 tggacaagtt catgctacaa aaaggttatt agaaaaatta gacaatgacc ctcttatctc
     4861 agctggatgg aaattgatgt catcaatttc catcacagtt gctctctaca tagcacttct
     4921 tgggtatttc attcatatga tctttttgtc aggtcaaatc aaatctagaa tgggtacttt
     4981 acgttcctta gggatgactt tatcgcaaag ctttagaatg atttttgttg agcagatact
     5041 attaattcta ttgggtgtag gtttaggaac ttggacggga gtgggcatga cccgtcttat
     5101 ggtttcagca attgctacta cggacaatgc tgtatccttg gtccctccgg ccttaatcac
     5161 gattgatttc ttgtcattgt taatatgtgc aggcactttt tttgccctat ttacgtttat
     5221 ggtcatatgg gtcagtcttt attttttccg actgaatctt gggactttgt ccaggttgga
     5281 agattaaatg atggagatag aggggtttgc gaaaattatc agggtttggc ccctaatagt
     5341 aaaacgattg gtagtgaatt ggagaacctt gtctaccgtg attattgggg tagttttggc
     5401 ttgttccatt atgtcaggca ccgtggtatt ttttgattca cttaaagaga tagccctggc
     5461 tgatgttctt aaatccctag ataaggaaga gataaacata ttagtgcagg ctgaaaaggg
     5521 tccaactaac gttgaggaag catcaatttt gaatgaaaga atccattctt tttccgaagg
     5581 attgctttct tctcatgtta atgaaatttt acatggagga agaaccacaa cctttttctt
     5641 cagttaccag ggccaggaac aagatgcagg aaaagataat atgcgaacat ttttcgcata
     5701 cctaccaaac ttgcagtccc atatcaccat aacggacggg ttgtttccgg ggaatgtcac
     5761 cggggaatta ttagaagata ttcctgttgt taaagtatta gtgaatgtcg aggacgctgc
     5821 aatttttgga cttcagattg gcgatcgcat atctgctgtg ccttattggg acgat
//
LOCUS       DASCNS010000087         1698 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000702564, whole genome shotgun sequence.
ACCESSION   DASCNS010000087 DASCNS010000000
VERSION     DASCNS010000087.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000702564"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="QF838_03170"
     CDS             complement(<1..556)
                     /locus_tag="QF838_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59726.1"
                     /translation="MYAKGYRRSRILGSFGDQSPPSLRTLGNWITRYKNVSEDILTLE
                     HDFEWPHMDMYSIPWEHAHLINRLSSLEHQKPSVRRVRWWFRTKEINPNYSDEVVGNI
                     ANQCVVNEHMDLMEIPGSDWSNLLESTLVDDEKQLELLPGTFAICSFELGTILPKWVQ
                     NGIFLSIIRTESKISVVCSDKSIPD"
     gene            complement(789..998)
                     /locus_tag="QF838_03175"
     CDS             complement(789..998)
                     /locus_tag="QF838_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59727.1"
                     /translation="MDFEEFLQHFRSDDLSYALKSLKLPTTGNKPDRVSRLVDLEKTG
                     TEVKNILRAFRVDDVKRAAKSVGLL"
     gene            1160..>1698
                     /locus_tag="QF838_03180"
     CDS             1160..>1698
                     /locus_tag="QF838_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HJO59728.1"
                     /translation="MTVKIVTDSTSDLDPALAQKLGITIVPLNVHFGQTIYKDGIDLN
                     TDQFFDKLINGTVFPSTSQPSLGEFVDVYEKVNQPGDVIISIHVSSKLSGTINSAHQA
                     AASLSDKIDVKIVDTQQVSMTVGLSALGAAKAAQEGQSAEECVAIAESIAHRSNFFAL
                     FDTLEYLEKGGRIGKARSL"
BASE COUNT          527 a          352 c          323 g          496 t
ORIGIN      
        1 catccggaat agacttgtct gaacagacta ccgaaatttt agattctgtc ctgataattg
       61 aaagaaaaat tccattttgc acccacttcg gtaaaatcgt accaagctca aaagagcaaa
      121 ttgcaaatgt ccccggcaac aattcaagtt gtttttcatc atctaccaat gttgactcta
      181 ataggttaga ccaatccgaa cctggtattt ccatcaaatc catatgttca ttaacaacac
      241 attggttagc aatatttcct accacctcat cagagtagtt aggattaatt tcctttgtac
      301 gaaaccacca tcgaacacgc cgcactgacg gcttctgatg ttcaagagaa gatagtctgt
      361 tgattaagtg ggcatgctcc catggtatag agtacatatc catatgtggc cattcgaagt
      421 catgttccaa tgtgaggata tcttctgaaa catttttata tctggtaatc caatttccca
      481 aggtacgtaa gcttggtgga gattggtcac caaaagaacc taggatccta gaacgccggt
      541 accctttggc gtacatttca aatcccctgg atatttgtgc agtattagca aaagttcttc
      601 ccataattaa tgcatatttt acatgaatta ctgcattttt tctagaattc gactttatta
      661 tttccttttt ttattttttt attgccttat ttacagacaa agatttttcc agtggtgcat
      721 aaataggtag aagaatatat aaaattattc gatacaatca ggaactggta ccggaacatt
      781 ccattaagtt atagaagtcc gacagattta gcggccctct ttacatcatc cacccggaag
      841 gctcttagga tattttttac ctcggttccg gttttttcca aatccactaa tcgagaaact
      901 cgatccggtt tattacccgt tgttggtaac ttaagtgatt taagggcata tgaaagatca
      961 tcagatctaa aatgttggag aaattcttcg aaatccatag aggtatctcc aatttgattg
     1021 aatgcaacaa aatatttcgc ccgattatac agacagttaa ccctgttgat aagccaaaag
     1081 gaaaatgacg ccgtacctag cggcataaga cacatcaaaa gtagcgtaga atgttactta
     1141 tttgagtggg agattaaaca tgacggtcaa aatagtcaca gacagtacct cagatttaga
     1201 cccagccctt gcccaaaaat tgggtataac gatagttccc ttaaacgtac attttggcca
     1261 aactatctac aaagacggta ttgacctaaa cacggatcag tttttcgaca agctgataaa
     1321 cggaacagtg tttccttcaa cctcacagcc atcgctgggt gaatttgtag atgtctatga
     1381 aaaggttaac caacctggag atgtgattat ctcaattcat gtatcttcaa agctcagtgg
     1441 taccatcaat tctgcccatc aagccgcagc cagtttgtca gacaagatcg atgtcaaaat
     1501 tgtagataca cagcaggtct caatgacagt tgggttgtcg gctctcggtg ccgccaaagc
     1561 ggcacaagaa ggacaatcag cagaagaatg cgttgccatc gctgagtcaa tagcccacag
     1621 aagtaatttt tttgccttgt ttgacacact ggagtaccta gaaaaaggtg gaagaatcgg
     1681 aaaagcgagg tcgttgat
//
LOCUS       DASCNS010000088        11868 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000710371, whole genome shotgun sequence.
ACCESSION   DASCNS010000088 DASCNS010000000
VERSION     DASCNS010000088.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11868)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000710371"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="QF838_03185"
     CDS             complement(<1..508)
                     /locus_tag="QF838_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012703.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HJO59729.1"
                     /translation="MGAPTDEGSPFMPGSRFGSRSIREQSLRFGANGVGYFDHDTNKV
                     FLEYELDNNRIVDLGDANVIPTDVEGTFENITNLTRMVLDKGAMPVVLGGDHAITFPV
                     VRAYNEPLHVVHFDAHIDYRPFVHGFEYTNSHAFRHIHQMDHVESLTQVGIRSIRNNK
                     AWIDDSIND"
     gene            823..1458
                     /locus_tag="QF838_03190"
     CDS             823..1458
                     /locus_tag="QF838_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59730.1"
                     /translation="MYIIDISSLKKEGECGSKKWGEACAAAAVKILKAADLPADFEWA
                     FTERYTHPPDRLMNGGRTQCGYYIMVRNGEITGGDGEPEEALAIRGFHIRARWAALCN
                     QSGAFYGAEGKLKRGNGEVVMREAIKRYLGREDPYGEVQPSERYFPEAVRGPLMAGSE
                     EGNGLHNIAASMQTSSPEFADFPVTEMLVPIFDEMSEEQKKSFINLLGIDI"
     gene            1812..2528
                     /locus_tag="QF838_03195"
     CDS             1812..2528
                     /locus_tag="QF838_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59731.1"
                     /translation="MGILVQREFTVTRDHRQEFERQSRLGTWVKMRHNGAQMIGFGGW
                     AFGGRGDVVVTHSTYADFDHWTATRPWGAFTTDPERAEETRHASAIFAGRPRLINHSR
                     TKIIDYDNELSEPTPFYRNSGDELASLPLTYGPQSILAECRYRLRSGSEAEFRELSAE
                     TLWPWLRAHDARMVLYGKDLLAAADEVVELTAYRSISEWHQLLNPTGDTKQVSAKRQS
                     LIADRQTRLLMVQTDFGEKV"
     gene            2559..3854
                     /locus_tag="QF838_03200"
     CDS             2559..3854
                     /locus_tag="QF838_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HJO59732.1"
                     /translation="MSKDKVVIAGGGIGGLVLALTLNQIGIECIVCESVRNPRPLGVG
                     INLQPNAVREMFDLGFTVADMNEFGVPAKEWALVGLKGQEIYSEPRGIFAGYSWPQYA
                     VHRGEFHMALYRRFRDLAGDDAIRLGCRAKGYDIHKNGTVSLQLDTPDGPDYENGTLL
                     VGADGIHSAIRGAMFPRQPPIHWGGAIMWRGTSRARPIRTKSSFVGLGNHRQRMVIYP
                     ISEADPNTGLALINWIAEVTYDDPSTHDNTDWFRSVEIESFIHHFEDWTYDWLDVPSL
                     IREAEEVFENPMIDRDPISHWAEGPVALLGDAAHPMYPTGSNGASQAIVDGRIMGAKF
                     LEHGVTPAALQAYNNMLCRPISEVVLRNREAGPFGLLNMVNDRCDGEFECIDEVISAS
                     ERSDFMGKYQAAAGFARDILNDAPPTIRPGARVGRMGRY"
     gene            4106..5245
                     /locus_tag="QF838_03205"
     CDS             4106..5245
                     /locus_tag="QF838_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020032898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO59733.1"
                     /translation="MSEQTSKEIRSTVTSEGYIEISIITVEKPAPSENEVLIKVEASP
                     INPSDLGLLISFAANLDSLSVSGSGDHTVAKMKIHEGLMKTMTPRMGQSMKVGNEGGG
                     VIEDAGANAKELIGKTVGAAGGAMYSHYRCVPKTSCLVMDDNTTSSEAASSFVNPLTA
                     LGFIETMKLENHSAIVHTAAASNLGQMLVKICKDDSVPLVNIVRRSEQVELLKNLGAE
                     HICNVSEPDFMEGLVDALEATGATLGFDATGGGNKGRLAGQILSAMEIAANKIAKEYS
                     RYGSDTYKQVYIYGGLDQSPTILNRSFGMQWGLGGWLLTPMIGKFGMETFQQMRERVV
                     KEIKTTFASHYTEEISFEEMLQPETIRAYAKQATGEKYLVAPHKE"
     gene            5403..6179
                     /locus_tag="QF838_03210"
     CDS             5403..6179
                     /locus_tag="QF838_03210"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO59734.1"
                     /translation="MRLEGKVALITGGARGQGAAETKLFAKEGAKVVIADILDEEGEK
                     LEAEITELGGECFYTHLDVSSSENWEETINTTVTHFGTIDILVNNAGIAVWGTNDDTT
                     EEIWDTVMDVNAKDVFLGTKRVIPEVKRSGGGSIINISSISGLVGQPTIQPVYNASKG
                     AVRLFTKSTAVQYGSYGIRANSIHPGAIDTDMISHRIQGEENKNRIKHTVPLQRVAKP
                     IEIAYGALFLASKESSYMTGSKMVIDGGVTAGAPNSSANS"
     gene            6513..7781
                     /locus_tag="QF838_03215"
     CDS             6513..7781
                     /locus_tag="QF838_03215"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59735.1"
                     /translation="MPGPLDGIKIVEFTQIIAAPFGGMLLSDMGAEIIKIEPIEGEPW
                     RLHTEFLPKESKTFIALNRGKKSLPLDLQSKEGLEIAQKLVADADVVIINARPDVPKK
                     LGIDYETLSEANPKLIYCDNTAFGRQGPHGYRPGYDLIIQAMSGLLAADNKVVDGVPQ
                     QVTATAVADYTTGVAIAWGVSAALFSRERTGKGQKIDTTLLSTSLLIQGGFMEVEEHG
                     KEEKDELVETLGALRQAGLPYQSLLDQQQIFSPFNTAFRIYYTTYQTANDVIAIACLS
                     NRLRKKAADAIGVVDPRFSEGYDPEDPSIVSAIESLSEEMKTILLSKTGEEWLSILDA
                     AGVPAGPVRFIQELTNDEQVIANDMVVEVNHSLAGKIRMAGPMIQMSETPLAVKSASP
                     ALGEHTDEIMLNLGYSEDEITDLKSRNITL"
     gene            7911..8144
                     /locus_tag="QF838_03220"
                     /pseudo
     CDS             7911..8144
                     /locus_tag="QF838_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
     gene            8323..11013
                     /gene="acnA"
                     /locus_tag="QF838_03225"
     CDS             8323..11013
                     /gene="acnA"
                     /locus_tag="QF838_03225"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HJO59736.1"
                     /translation="MNNNGNPSSLISKNILQTLSGSYTYYSIDSAEKAGLINIDKTPY
                     AIRVLLENVIRNADGGPATEDHIKLVSSWRPNNKPASEFPYMPGRVLLQDFTGVPVAV
                     DIAAMRDAVSELGADASIINPIVRSDMVIDHSVQVDNFSVPEALNLNIEMEFERNTER
                     YQLLKWAQNAFDNFKIVPPGTGIVHQVNLEYLAEVVLSGDDVVNPVAFLDTCVGTDSH
                     TTMVNGLGVLGWGVGGIEAEAVMLGQPYYMVVPEVVGVRLTGTLPNGSTATDLVLSIV
                     EMLRAVGVVEKFVEFYVPGLKELSLADRATIANMAPEYGATCGFFPIDHQTLRYLKDT
                     GRDTAIVELVEQYSKMNNFYYDDQKEPDYSVDINFDISKVIPSMAGPKRPQDKISLEN
                     IPENFSESFSDSSGRIHKIEIDEEEVEFGDAAVAIAAITSCTNTSNPSVMVGAGLLAR
                     NARQKGLQRKPWVKSSLAPGSTVVTRYLEAAGLNEDLDYLGFNTVGYGCTTCIGNSGP
                     LPQPVAEAVSDNDLTVAAVLSGNRNFEGRVHPQVKANYLASPMLVVAYSIAGRVDVNL
                     VEDSIGQDPSGNEVFLKDIWPTQDDISNTISSCLTPEMFQEQYGKVVEGPQEWQNLET
                     SSTLNFNWDKESTYIQKPPYFENFTKNPSERTEITDARVLVYLEDSVTTDHISPAGSI
                     PPSAPAGQFLLGNDVPRREYNSFGSRRGNHDVMVRGTFANIRLRNKLNPAMEGDWTVH
                     QPSEEIMRIYDASEKYKNEEVPLIIIAGKEYGTGSSRDWAAKGPMLLGVRAVIAESFE
                     RIHRSNLIGMGVLPLVFINGQTAMSLGLDGTETYDIPGVANNVTPSSNIEITATSKTG
                     KVTKFEVLARIDTEIENEYYRHNGLLPYVLRQMMSENSVS"
     gene            complement(11044..>11868)
                     /locus_tag="QF838_03230"
     CDS             complement(11044..>11868)
                     /locus_tag="QF838_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HJO59737.1"
                     /translation="MLLMSATPIPRSLYFTMIGDLDLSVIDQMPVGRKPVQTYVYEQN
                     KREEVHRFIRKEILKGRQAFVVCPLIEESESLQSRAAIEEFEKLSKDIFPEMKVGLLH
                     GRMKLPEKESVMNEFRSGKLHILVATSVIEVGVDIPNASVMLIDGAERFGLSQLHQFR
                     GRVGRGEHQSYCILMSDNPSADSKERMEILHRINDGFRLADEDLRMRGAGDLIEGRRQ
                     SGTPFFRVADPSDADLVSLAGIEASKVLNLDYELSSEEHQALRIQLDERLKELSIT"
BASE COUNT         3423 a         2525 c         2769 g         3151 t
ORIGIN      
        1 catcgttgat agagtcgtca atccaagcct tgttgttgcg gatactacga attccaacct
       61 gtgtaaggct ctcaacatgg tccatctggt ggatgtgacg gaaagcatgg gaattagtgt
      121 attcgaagcc gtgaacaaag ggtctatagt cgatatgggc gtcaaagtgg acgacatgga
      181 gaggctcatt gtatgctcgg acaactggaa aggtaatagc atggtcgcct cctaggacca
      241 ctggcatcgc gcctttatcc aacaccatcc gagtcaggtt agtaatattc tcgaaagtac
      301 cttcaacgtc agtgggaatg acattcgcgt cacccagatc tacaattcga ttgttatcaa
      361 gttcatattc taggaacact ttgttagtgt cgtggtcaaa gtagccaacg ccattagccc
      421 cgaatcgtag agattgctcc cttatcgagc gtgacccgaa tcgtgaaccg ggcatgaatg
      481 gagagccttc atccgtcggc gcgcccatca cggcaatatc tgcgtccaac tggtcaagat
      541 catggcagac tggtgttcta agaaatgaga ctaatccagc aaacccatag tttggtttac
      601 ctcggttggt cggatgttgc gccttacgct gtgtcattta ttgattcctt tttcctcatt
      661 tctttctgaa tcatgtatat cggattcctt cccgattgtt ggcaatgata cctgttaatc
      721 ggcttatatg tgagattgtt tattcgatta ttacaaacca tgtagtatat tcaacgtaag
      781 ttgagtttga tattagagaa cccatagaaa tacaagaata ttatgtacat tatagatatt
      841 tcgagcttga aaaaagaggg tgagtgtggg tctaaaaagt ggggcgaggc ctgtgcggct
      901 gcagctgtaa aaattcttaa ggcggctgac ctgcctgctg attttgaatg ggcctttact
      961 gagcgctata cccatccgcc agatcgactg atgaatggtg ggcgcacaca gtgtggttac
     1021 tacatcatgg tgaggaacgg tgaaataacg ggtggtgatg gtgaaccaga agaagcactt
     1081 gcgatccgtg gatttcacat ccgagcacgc tgggcagcat tgtgtaacca gtctggtgcc
     1141 ttttatggtg ctgaagggaa gttaaagcgc ggtaacggcg aagttgtaat gcgtgaagca
     1201 attaagagat atcttgggcg tgaagatcca tacggtgaag tgcaaccgtc agaacggtat
     1261 ttcccggaag ctgtacgtgg tccattgatg gcagggtcag aggaaggcaa tggacttcac
     1321 aatatcgccg cctctatgca aacctcatct ccggagtttg ctgattttcc agtgacagag
     1381 atgctggttc ctatttttga tgagatgagc gaagagcaaa agaagtcatt tatcaacctg
     1441 cttggcatcg acatctaatc tttctttttc cacctacttc cttacaacat ttggatactt
     1501 aatttcgaga gacatacgtg aaagcaatac cgaggcatct gaaaatattt ttccttggca
     1561 ttatttctgt catcacccaa agatcccaag ttcgagtcat gccctcacta ccactcattt
     1621 gtgactaaaa tccctttttt tgcctcctac ctcacttttc aaattagaga tttccgtaaa
     1681 tatggttacg aactttctgg aagcgtcaga tttaatgctt cgtcaatttg tctcgtagca
     1741 gcttcttcca aaccagtcgt tacatggaaa taagtgtcta taatgaataa cacaatccat
     1801 aaggagggaa tatgggcatt ctggtacagc gggaattcac cgtcactcgc gatcaccgac
     1861 aggagtttga gcgtcagagt cgtcttggaa catgggtgaa aatgcgccac aacggcgcac
     1921 agatgatcgg tttcggaggc tgggcgttcg gtggtcgggg ggacgtcgtg gtcacccatt
     1981 cgacctatgc ggatttcgat cattggaccg caacgcgccc ttggggcgca ttcacaacag
     2041 atcctgaacg tgctgaagaa actcggcacg ccagcgcgat ctttgccggt cgtccacggc
     2101 tcatcaatca ctcccgtaca aagatcatcg actacgacaa cgagttatcc gaaccaacgc
     2161 ctttctaccg caatagcggt gatgagttag cctctctccc gctgacctat ggtcctcagt
     2221 cgatacttgc agagtgccgt tatcgccttc gctcgggatc cgaggcagag tttcgcgagc
     2281 tgtccgcgga gacgctttgg ccttggcttc gagcgcatga tgcacgtatg gtgctctacg
     2341 gcaaggatct gctggcggcg gcggacgagg tggtggagtt aacggcctac cgttcgatta
     2401 gcgaatggca tcaactgttg aacccaactg gagacacgaa gcaggtctct gccaagcgac
     2461 agtcactgat cgcagaccgt cagacgcgac tcctgatggt gcagacggat ttcggcgaaa
     2521 aggtataggg atcgagctca tcaaggagta tgggggcaat gtcgaaagat aaagttgtta
     2581 tcgcaggtgg aggtattggt ggtcttgtac ttgcattaac actgaatcag attggtattg
     2641 agtgtattgt ctgcgagagc gtgcgcaacc ctaggcctct tggcgtcggg attaaccttc
     2701 agccgaacgc agttagggag atgtttgacc tcggattcac cgtcgcggac atgaatgagt
     2761 ttggtgtacc ggctaaagag tgggcacttg tcgggctgaa aggtcaggaa atatattcag
     2821 agccgcgcgg gatatttgct gggtattcct ggccgcagta tgcagtgcat cgaggagagt
     2881 tccacatggc actctatcga aggttccggg acctggcggg agacgacgca atacggcttg
     2941 ggtgtcgggc taaaggatat gatattcata aaaatggaac cgtctcgcta cagcttgata
     3001 cgccggatgg acccgattat gaaaatggaa ccttgcttgt tggagcagat ggtattcatt
     3061 ctgcaatccg tggcgctatg tttcctaggc aacctcctat ccattggggg ggcgctatca
     3121 tgtggcgtgg cacttcgcgg gcgaggccaa ttcgaacgaa atcatccttc gtaggtctcg
     3181 gtaaccaccg ccaacgcatg gtgatatatc ctatttctga ggccgatcca aatacaggtc
     3241 tcgctctaat caactggatt gctgaagtca cttatgacga tccgtcaacg catgataata
     3301 ctgactggtt ccggagtgtg gagatcgaaa gttttatcca tcattttgag gattggacct
     3361 atgattggct agatgtacca tcactgatac gagaagcaga ggaagttttc gaaaatccaa
     3421 tgattgaccg tgacccgatc tcgcactggg ctgaagggcc agttgccctt ctcggggatg
     3481 ctgcacatcc aatgtatccg acaggatcaa acggagctag ccaagctatt gtcgacggac
     3541 gcatcatggg cgcaaaattc ctagaacatg gtgtcacacc agccgccctg caggcctata
     3601 acaacatgct ttgcaggccg atttcagaag tggtattacg caatcgtgaa gccgggccat
     3661 tcggtttgtt gaatatggtt aatgaccgct gcgatggcga attcgaatgt attgacgagg
     3721 taatctcggc ctcagaacgg tccgacttta tgggaaaata tcaagctgct gcaggttttg
     3781 cgcgcgacat actaaatgat gccccaccga ccatcaggcc aggggctcga gttggcagga
     3841 tggggcggta ctaaataagc ggctgggtga aactattgtt aatgaatgaa aattatgcga
     3901 ttatgtcagc accctgatct ttggattttc atagctcata actcaaagat cgcaggttga
     3961 aatcttgctc ccgctgtcac tcattcatga ctaaaatttc cgcttttgac ccctactaca
     4021 cctcggaaat ggggcatgct ggcaatataa acacaagcga cgaatagtta ggctacaaat
     4081 tgttgattcg aaaggaaaaa taagaatgtc agagcaaact tcaaaagaaa taagatcaac
     4141 tgtaacaagt gaaggatata tagaaatctc tattattact gtagaaaaac cagcgccttc
     4201 agaaaatgaa gttttaatca aagtagaagc ttctcctatt aatccatcgg atttaggact
     4261 tcttataagt tttgcagcca atcttgatag tctgagtgtt tcaggttcag gtgatcatac
     4321 ggtagcaaaa atgaaaatcc acgaaggttt gatgaaaaca atgactccaa gaatgggcca
     4381 atctatgaaa gtaggcaatg agggtggcgg ggtgattgaa gatgccggcg caaatgcaaa
     4441 agaacttata ggaaaaactg ttggcgccgc tggtggagct atgtattccc attacaggtg
     4501 tgttccaaaa actagctgtt tggtgatgga tgacaacaca acttcttctg aagctgcctc
     4561 ttcctttgta aatccattaa ctgctttagg cttcattgaa actatgaaac tagaaaacca
     4621 cagtgccata gtacatacag ctgcggcctc aaatttagga caaatgcttg taaaaatctg
     4681 taaagatgac tctgttcctc ttgtaaatat cgttagaaga tcggaacaag tggaacttct
     4741 taaaaacctt ggtgctgagc acatatgtaa cgttagtgaa cctgacttca tggagggcct
     4801 ggttgatgct ctcgaagcga ctggtgctac tctaggtttt gatgcgactg ggggaggaaa
     4861 taaagggaga cttgcagggc aaatactctc agcgatggaa atagctgcga acaaaattgc
     4921 taaagaatat agtcgatatg gttcagatac ctataaacaa gtatatatct acggtggact
     4981 agatcaatca cccacaattc tgaacagatc ctttggtatg caatggggac ttggcggatg
     5041 gcttttaact ccaatgatag gtaaatttgg catggaaaca tttcaacaga tgcgtgagcg
     5101 agtagttaaa gaaattaaga ctacttttgc tagtcactac actgaagaga tatcttttga
     5161 agaaatgctt caaccggaaa ctattagagc ctatgcgaaa caagctaccg gtgaaaagta
     5221 tttagttgct ccccataaag aatagatcgt tatttccgtg taaaatcgcg ctaaaaatac
     5281 agcccaataa accctttacc gacaattggg cattaatagt gctcctaatc attccctcat
     5341 tctgctgtat tattcagtcc agtggatcat gtaatttatg aggaaggtga ggagaatttt
     5401 atatgagact tgaaggaaaa gttgctttaa taactggagg agccagaggc caaggtgcag
     5461 ccgaaacaaa gttatttgcc aaggaaggcg caaaggtagt catagcggat attctcgatg
     5521 aagaaggtga aaagctcgaa gcagagatta ctgagctggg tggggaatgt ttctatactc
     5581 acctagatgt ctctagctct gaaaattggg aagagactat caacactaca gtgactcatt
     5641 ttggaacaat tgacatactc gtgaataatg ccggcattgc agtctggggc accaatgacg
     5701 acacgactga agaaatttgg gatacggtta tggatgtaaa cgcaaaagat gtctttttag
     5761 gtacgaaacg cgtaatccct gaagtgaaaa ggtcaggtgg aggctcaatc atcaacatat
     5821 cgtccatctc tggcctagtt ggccaaccaa ctatacagcc agtctacaac gcctcaaaag
     5881 gcgcagtcag attgttcacg aaatccacag ccgtacagta cggcagctat ggaattaggg
     5941 ctaattccat tcatccaggt gcaatcgata cagatatgat ttctcataga attcaaggcg
     6001 aagaaaataa aaatagaata aaacatactg ttccacttca acgagtcgca aagcctatag
     6061 aaatcgccta cggagccttg tttttggcct caaaagaatc ttcgtacatg acgggcagca
     6121 aaatggtgat cgacggtgga gttaccgctg gagctcctaa ctcaagcgcc aattcataga
     6181 tctctagact tcctgcgact gaggtcatag gtatgttaca gaaaggcctc aagggccgtg
     6241 gcattttttg tatttttttc cactaccaca gggacatttt tcatttcgac ctattttcct
     6301 agatgcattt atctttatac ggttcgattc ttggcatttt gcgcaggtgc aagccacggg
     6361 atgttcttta tgccagttgg tacgcgatgt cacgagatat gtcctgggta tttaaaaaca
     6421 acacagccat gtaatttgac taagttatgg tttttcataa cattatcaca taccatagtc
     6481 aggtaaattg aattcattat tcggagatta atttgccagg tccgttagat ggaataaaaa
     6541 tcgttgagtt cacccaaata atagctgccc cgtttggagg catgttattg agtgacatgg
     6601 gtgctgagat aattaaaatc gaacccattg aaggcgaacc ttggagactc cacactgaat
     6661 ttctacccaa ggaaagtaaa acattcatag ccttgaatcg ggggaaaaaa agtcttccgt
     6721 tggatcttca aagtaaagaa ggtcttgaga tcgcccagaa acttgtggca gatgccgatg
     6781 tggttatcat aaacgctcga ccagacgtcc ctaaaaagtt ggggattgac tacgaaaccc
     6841 tatctgaagc caacccaaag ttaatatatt gcgataacac agcattcggc cgacagggcc
     6901 ctcatggcta tagaccaggt tacgatctga ttattcaagc tatgagtggt cttttagctg
     6961 ctgacaataa ggtcgttgat ggtgtcccac aacaggtgac tgcgactgcc gttgcagatt
     7021 acaccacggg agttgcgatt gcctggggtg ttagcgcggc cttattttcg agggaacgta
     7081 ccggtaaagg ccaaaaaatc gacacaactc tgctttcgac atctctactc atccaaggtg
     7141 ggtttatgga ggtggaagaa catggaaagg aagagaaaga tgaactggtg gagactttgg
     7201 gtgccttaag gcaagctggc ctgccgtacc aaagcctgtt ggaccaacaa caaattttct
     7261 cgccatttaa tacagcattt cgtatctatt acaccacata ccaaactgcc aatgacgtga
     7321 ttgccatcgc ttgcttaagc aacaggctta gaaaaaaagc tgccgatgca attggtgtgg
     7381 ttgatccacg gttttcagaa ggctacgacc ctgaagatcc atccatagtg tcggcaatag
     7441 aatccctatc ggaagaaatg aaaaccatat tactgagtaa aaccggtgaa gaatggcttt
     7501 cgatccttga tgcagcggga gttccggctg gtcctgtgag gtttatacaa gaactgacta
     7561 atgacgaaca agtgattgca aatgatatgg tggtagaggt aaatcactca cttgctggga
     7621 aaattcggat ggctgggccc atgatacaaa tgagcgaaac ccctttggcg gtgaaatcgg
     7681 catctcctgc tttaggggaa catactgatg aaatcatgct caatctcggc tattctgaag
     7741 acgagattac cgatctaaaa tcccggaata taaccctctg acattcatat ttctcgccaa
     7801 tcaaacaatt cacagcagtc tccacgttga agccctcata ctcaagagat ataattccga
     7861 ggttatccca taaggaattt tcatttagac gaggagtaca aaaatagcat atgacctaca
     7921 ttatcgctga accctgtatt gatgtgttag atagagcctg catagatgta tgcccggttg
     7981 actgcattta tgaagcagaa gagcatagta tgctacttat aaacccagaa gagtgcatcg
     8041 attgcgctgc atgtgagcca gtttgcccag taaccgccat atttgccgag gacgacgtgc
     8101 ctgccgactg ggataaatgg actgagctga attacgagta ctttggacag aaaagataac
     8161 aaatatgggc tttactcgcc ctcttttaaa ttgaagttgg ttttaacgcg gattaacctc
     8221 tgggttgaat attaatagct atcgtgataa tttcactcag atgagatact attcctgagt
     8281 tgccgactgc ctcatcctta gcaatttcgg agttacagtc tcatgaataa caatggtaat
     8341 ccttcaagcc taatatcaaa aaatattctg cagaccctat caggtagtta cacctattac
     8401 tctatcgact ctgccgagaa ggctgggtta ataaacatcg acaaaacccc ttatgcaata
     8461 agggttttgt tagagaatgt tatacgtaac gccgatggag gccctgcaac ggaagaccat
     8521 ataaaattag tatcatcttg gcgcccaaac aataaaccgg ctagtgaatt tccttacatg
     8581 ccaggtagag ttctactcca agatttcact ggagttccgg tagcggtaga catagcagct
     8641 atgagagatg cagtgagtga gttgggagct gatgcatcta tcatcaatcc aattgtccgc
     8701 tcagatatgg ttatagacca ctcagtccaa gtagacaatt tctctgtccc cgaggcactt
     8761 aacctgaata tcgaaatgga atttgagaga aatactgaaa gataccagtt gctgaaatgg
     8821 gctcaaaacg cctttgataa ttttaaaata gttcctcctg gcaccggaat cgttcatcaa
     8881 gttaacttgg agtacttggc agaagttgtt ttaagtggag atgatgtagt caaccctgtt
     8941 gcatttttgg acacatgtgt gggaactgac tctcacacta cgatggtcaa tggtttaggg
     9001 gttttaggat ggggggtggg tggaattgaa gcggaagcag tcatgctggg gcaaccatac
     9061 tatatggtcg taccagaggt agttggggtg agactgactg gaaccctacc aaatggctcc
     9121 acagcgactg atctagtttt atcgatagta gaaatgctac gagcagtggg tgtagtcgag
     9181 aaatttgtcg aattttatgt tccaggattg aaagaactaa gccttgctga tcgagccaca
     9241 atagcaaaca tggcccctga atatggggca acgtgtgggt tttttcctat agatcatcaa
     9301 actctaaggt acctaaaaga cactggacgt gatactgcaa tagtggaatt ggttgaacag
     9361 tactcaaaaa tgaataactt ttattatgat gatcaaaagg aaccagatta cagtgtagat
     9421 atcaattttg atatttctaa agttattcca tcgatggcag gacctaaacg accccaagac
     9481 aaaatttcct tagaaaacat tcctgaaaat ttttcagaat ctttctctga ttcatcaggt
     9541 cgcattcaca aaatagaaat cgatgaagag gaggtggaat ttggggatgc tgcagtagca
     9601 attgcagcta taacaagttg caccaacact tcaaacccct cagttatggt gggagccggt
     9661 ttattggcgc gaaacgcgag acaaaaagga ttacaaagga aaccatgggt taaatcgagt
     9721 ttggcaccgg gttccactgt agtaactaga taccttgagg ctgccgggct aaatgaagac
     9781 ctagattatt taggttttaa cactgttggg tatggttgca caacctgcat cggaaattca
     9841 ggaccgctac cacaaccagt cgcggaagca gtctctgaca atgatttaac cgtggcagcc
     9901 gttctaagtg gtaacaggaa ttttgaaggt agggttcacc cacaagtgaa ggccaattac
     9961 ctggcatctc caatgctcgt ggtggcctat tctatagctg gtagggtaga tgtcaatctg
    10021 gtagaagatt ccatcggtca agacccatca ggaaatgagg ttttcttaaa agacatttgg
    10081 cccacacaag acgatatttc aaacacaata tcctcatgcc tcacaccaga gatgttccag
    10141 gagcaatacg gtaaggtagt cgaaggcccc caagaatggc aaaatttaga aacgtcttct
    10201 actttaaatt tcaactggga caaagaatcc acatatattc aaaaaccacc atactttgaa
    10261 aactttacca agaacccttc tgaaagaaca gaaatcaccg atgccagagt gttagtgtat
    10321 ttggaagatt cggtaaccac ggatcatatt tcaccagcag gatccatacc tccgagtgct
    10381 ccggcaggcc agttccttct aggaaatgac gtacctcgac gggagtacaa ttcattcgga
    10441 tctagaaggg gtaatcacga tgtaatggtt aggggtacat ttgcaaatat tagacttaga
    10501 aataagttaa acccggctat ggagggtgat tggactgttc accagccttc ggaagaaatt
    10561 atgcgtattt atgacgcttc agaaaaatat aaaaatgaag aagttccttt gataatcatt
    10621 gcaggtaaag aatacggcac cggcagctcg agagattggg ccgctaaagg ccctatgctc
    10681 ctaggagtca gggccgtaat agccgaaagc tttgaaagaa tccaccgctc gaatttgatt
    10741 ggaatgggcg ttttgccatt agttttcata aacggacaaa cagcaatgtc acttggttta
    10801 gatggaacag aaacatacga catacctgga gttgcaaata atgtcacccc ttcttccaac
    10861 attgaaatta ctgccacctc aaaaacaggt aaggtaacta agtttgaggt tttggcgcgt
    10921 atagacactg aaatcgagaa tgaatattac aggcacaatg gactcctgcc ctatgtactg
    10981 cgtcaaatga tgtctgaaaa ttcggtttca taaatactga ttcaaggtcc gaatttatag
    11041 ctctcaagta atactaagtt ctttaagccg ttcatcaagt tgaatcctca atgcttggtg
    11101 ttcttctgaa gacaattcgt aatctaaatt caagactttg gaagcctcta ttcctgccag
    11161 tgatacaagg tccgcatcag atggatctgc gactctaaaa aacggagtcc cactctgcct
    11221 cctaccttct attaaatctc cagcacctcg catcctcaag tcttcatctg ccaacctgaa
    11281 cccatcattt attctatgta atatctccat gcgttctttc gaatcagcac tcgggttatc
    11341 agacattaga atgcaataac tctgatgttc acctctaccg actcgtcctc ggaattgatg
    11401 taattgagat aacccaaacc tctcggcacc atcaatcaac attacactgg cattaggaat
    11461 atctaccccc acttcgatta cggatgtggc caccaaaata tgtagtttac cagaccgaaa
    11521 ttcgttcatt actgattctt tttctggcaa tttcattctt ccatgtagca atcccacttt
    11581 catttcagga aaaatgtcct ttgacaattt ttcaaactct tcaatggctg cccttgactg
    11641 tagagattct gattcttcaa tcagggggca aacaacgaat gcttgtctac ccttcaaaat
    11701 ttcctttcgt ataaaacgat gtacttcttc acgcttattc tgttcataaa catatgtttg
    11761 aacaggtttc cttccgactg gcatctggtc tatcacagac aaatctaaat cgccgatcat
    11821 cgtaaaatat aaggatctcg gtatgggtgt cgcggacatc agtagcat
//
LOCUS       DASCNS010000089        16899 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000711839, whole genome shotgun sequence.
ACCESSION   DASCNS010000089 DASCNS010000000
VERSION     DASCNS010000089.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 16899)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16899)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16899
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000711839"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="QF838_03235"
     CDS             <1..1295
                     /locus_tag="QF838_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59738.1"
                     /translation="NYPAEYMTSFMNSYMDKKDRLIAAVADCRRMGIEILPPDINRSY
                     SKFTIEENLESGKAIRFGLAAIKNIGSEALRSFLDSRDQTGPYETLAQLCHDGDISSL
                     NRKAIECLVMSGSFDSFGDRTGLLEVSDRISSLAQDEANIRNSNQSTMFEMLGDSVSS
                     ALSSIDIPFTSTSDHQKRLWEVELMGISISGAGNLGKLLSGFGKDVSVMLTQLQGVSP
                     AGFTVLAGQISTVVDRFTRDNRPFKVVNIEVLDGSLEAVVWEDVLSKTAELWEPGRIV
                     KMKGNLRERDGEVTISVTEANEINLDKAFNNTNDTSANPEENGAILHTNSPMINTNGD
                     GNHSNGSDVPTNRKKLILSIRESNNTTNDQMLLDDIKRLLLSASGNDEVGLEIETESS
                     VVVMEWPPVKINATPELESKLSALVGSTGKVRIQSLMF"
     gene            complement(1284..1814)
                     /locus_tag="QF838_03240"
     CDS             complement(1284..1814)
                     /locus_tag="QF838_03240"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HJO59739.1"
                     /translation="MKPHHLYNTKFETSLGWIALERSELGITRCSLPERQESKCIQEI
                     KGWKSEYLETGDLFSKEIEKLHGYLEGQDYSLQDIVIDVSTAPRFYKKAWAICRSIEI
                     GTTKSYKWVADKSGSPKASRAVGQAMANNKITLIIPCHRVIGANGNLTGFGKGKTRLD
                     LKLELLKLEKQYISKH"
     gene            complement(1792..4332)
                     /locus_tag="QF838_03245"
     CDS             complement(1792..4332)
                     /locus_tag="QF838_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013961.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HJO59740.1"
                     /translation="MIKEVRISELLKPGQAPPTKWANSISNLKTFIPEQGITLYVILL
                     LSLICTADIVVIGNWVETPGIYSMVLLSSLFPLFFHRIKLHPLLIHLISFSIGTILVL
                     YNTLTLIKDLPVNEKVSELKLRLDYWYGIASTEGISTDLIPYTIFLLSLAWVLGYTSS
                     WFAFRHNNIWISIVLNGMSILTCLSFLPEQYSSRFYLYAFTAMILITHITTVKQRGEW
                     SKNGTVFNQLDGWLTLNSAIWYSIAILIIATAIPLKIYVSRPVADTWKAAREPVAMIE
                     NEFARLLGSVPSRTQQNGRFFGKFLPFIGSISFREQGVLTAKSDYPNYWVSRTYNTYT
                     HEGWISGETHTIDLDKYSLFDNTTGLQSTKSVSQSIEMSFPSDQFFAGGNISSVSEPI
                     VLESLKPKTFEIEVGDGKFDGYLPSDLQQFESHIEKFLEEYPTDIRNNKFEEHLLSQL
                     PDEYKYVNTSANNNGTEKIKLTRTVPLNLDIVNWKLARQIPANKQLMMVSSVSVSKPN
                     ELRTAGQKYHGFIQDHYLELPENMPKRIYDLTTKITSGKTNPYDKAVAIEEHLRNGDY
                     QYSHKISRPPIGADGVDHFLFTTLTGYSDYFASAMTVMLRISGIPARLATGYSSGTYL
                     PDKNSSTIKDSDSHGWVQVYFPNYGWIDFEPTPNWSKYSRLQREKTPSAFIRSGSVYQ
                     DGNYPKLPDDFYDELGPDSEVSENKRGTVYFILKKSFLVPISIIFSIVLLSFGLNTFW
                     QWGLSGLSQAEKLTIKMYRIGALVGTSKTKYQTISEFSSKMSIKYPSIQTEIYTIAYA
                     FMKEKYSPQSSYSENLDTCWKTIRNYLWREIVGRSFKWTQKNETTSPL"
     gene            complement(4329..5540)
                     /locus_tag="QF838_03250"
     CDS             complement(4329..5540)
                     /locus_tag="QF838_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013998.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HJO59741.1"
                     /translation="MVLLIITVITALATGFELFFRLAYIIIIVSLVSLISVYLNIRKI
                     EVSIDRRNHLLSVGEQIDKRISIRNLSVIPKTLILATDITTIPEYTSSFALGLTAKGY
                     RSWRSTDYARQRGVFEMGPIEISTTDLLGIYKASTRHGEIDKIMVYPKIYDLRNFNIG
                     TSQITNEGTSRKKSNILSPHASSVREYAYGDSLSRVHWKTTARANRLMSKEFDVGSSN
                     EVVILNDLDKSVQCGRLDDSTDELSISVTASLTKSYTDSHTPVGFLSHGNDRYYITPG
                     IGSSHFDRTMQTLAVAKPGNGKKLQQVIAEERNIWFNNSSIIIITPSSETEWVTALSE
                     LSQFNTSITVIMVNAKSFGGKHELGPILSALDNIGISPYVINKNDELNESLSRSFFKR
                     SVQNLSMTKSS"
     gene            complement(5590..6576)
                     /locus_tag="QF838_03255"
     CDS             complement(5590..6576)
                     /locus_tag="QF838_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HJO59742.1"
                     /translation="MTEKTNHSCNEAAKTANLITDNINHVIVGKQTSVELATIALMSG
                     GHILVEDVPGVGKTVLARSLAISSGCTFKRIQFTPDLLPTDVSGVSIYNQKSGDFEFR
                     EGPIVSQIVLADEINRATPKTQSAMLEAMEENQVTMEGITRELPKPFMVIATQNPIEY
                     EGTFPLPEAQLDRFLLKVNLGYPNEVDEIAILSKQSLTHPIDGISSVVTPKMVTDMQE
                     AVKSVYVDDLIKEYIVKIVSQTRNVLELSLGSSPRGSIALHRASQAMAIIRERDYVLP
                     DDVKHLAPSILAHRLIPSPSARMNGMEPEQVIQDLMKSTSIPGERSQSWFRR"
     gene            complement(6826..7233)
                     /locus_tag="QF838_03260"
     CDS             complement(6826..7233)
                     /locus_tag="QF838_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJO59743.1"
                     /translation="MSDHPLTFKATKEDTNGAYSLFEANLVGGGPGQHIHKNEDEALY
                     VLEGEINIKLGDDTFVAQAGSFVFLPRNIVHTFWKANEQPTKILVIISPPQFENFFFE
                     AVGEEEIDGQTFGERALQLADKYKVQFIGPPLG"
     gene            complement(7345..7421)
                     /locus_tag="QF838_03265"
     tRNA            complement(7345..7421)
                     /locus_tag="QF838_03265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7385..7387),aa:Val,seq:tac)
     gene            complement(7433..7506)
                     /locus_tag="QF838_03270"
     tRNA            complement(7433..7506)
                     /locus_tag="QF838_03270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7470..7472),aa:Arg,seq:gcg)
     gene            complement(7597..8034)
                     /locus_tag="QF838_03275"
     CDS             complement(7597..8034)
                     /locus_tag="QF838_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016001.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HJO59744.1"
                     /translation="MNLQSTLIVAFSKFHSLILRLTRGKFMERLAGLDMLLLTTVGRK
                     TGKKRYTTLLFKKIDGHYYCAGSFGGSPKAPQWYRNIFSNPDVEILAEGKFLNATAVI
                     LEDDLKAIAWESLIDIYPNFQKYQNKTDRVIPVIKFQYNPNRD"
     gene            complement(8046..9128)
                     /locus_tag="QF838_03280"
     CDS             complement(8046..9128)
                     /locus_tag="QF838_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HJO59745.1"
                     /translation="MINLESLLLELSNAPGPSGFENPVREIMKRELYPFVDSLNTDGM
                     GSLIGIKNGSSSSLNIMLSAHMDEVGLLVRFITDEGFIKFQNLGGWLDQAIIGQRWQI
                     LTSKRTVLGITGIKTPHVMSTDERGKVFKGENLFLDVGAQNKADAENRLGIKPGDPIA
                     PVSDATRLNDSEFILGKAWDDRAGLAVLVGVMESVKDSDLPFNICAVATVQEEIGLRG
                     AHTSSYAVNPDIGLNLESGVAGDYPSISQYEAQEKLGNGPAIFLHDSSMLPNLKLRDF
                     VIDTAKDSDINLQFNVLSGYGQDGAEMQKSFEGAPAINITVPTRYLHSHNSIISIKDV
                     EKAIGLVGQCILQLNLEKIKNLKNFD"
     gene            9312..10484
                     /locus_tag="QF838_03285"
     CDS             9312..10484
                     /locus_tag="QF838_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019640.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO59746.1"
                     /translation="MVEIKTDSMPNRKFSFLDVAKEFVPKILDASRVISEGREIPPQL
                     ASEMAEEGLFRLYIPKSVGGYEIDYLEFLELVSVIAEADGSVGWCFNQNNVLSTLSAF
                     MPKELAQEIWSDPTVVLSNGPPISPVAVPVEGGYQLNGRWNFSSGSRHAKWVVALAPV
                     KGRVLEDGESPEMRNMMIPKEEVEFIDTWEVNGLRGTGSFSFTAKDLFVPESHSFVEG
                     GPPREEGPIYLIPKSLLFCSGFATTALGVARGALNAVIELSSAKTPQEQDLLMKQPFT
                     QREIGQAEAIWRSANAYLRDSSEAVWESAVNDRVIPVETRINLRLSSTHAIREAVKVA
                     DMAYSLSGATAIFETSPIQRRMQDVRVISQQIQGRMSHYDTAGQYFLGMDPKSRLF"
     gene            complement(10481..11677)
                     /locus_tag="QF838_03290"
     CDS             complement(10481..11677)
                     /locus_tag="QF838_03290"
                     /inference="COORDINATES: protein motif:HMM:NF020084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HJO59747.1"
                     /translation="MDWSSKISESENFETGVRECLSAIEADLEAQPHLLIAFISPDFQ
                     ALYQDLPGLVSEYFPETTLIGCSGNGVIGEAIEVENRPEIALTAAMMPDVSITPFHIT
                     GTELPDGDQPPEKWESLLKVKRDSEPKFIILADPFTVEIQSLIKGMDYAFPDSTIIGG
                     IASGGSQPRSNAIYLNGTTDQGVAGIALAGDVEIDTIVAQGCKPIGSLMRITGCERNI
                     LTGLDDHNPFTVLSEIYNQLSEPDQQLFQNSLFLGTVTDPFIDPPTTKDFLIRNIVGA
                     NPDEGKLAIGEMLREGQLVQFHLRDSNTSSENLKIMLENYNSDQSEQSQFGALLFSCL
                     GRGAYLYGEANHDTNMFKEVVGEIPLTGFFCNGEVGPVGESTYVHGYTSSFGIIKPKK
                     LLSDKT"
     gene            complement(11680..12405)
                     /locus_tag="QF838_03295"
     CDS             complement(11680..12405)
                     /locus_tag="QF838_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO59748.1"
                     /translation="MSNKIISNMKEGKKSYGISLSFYADEMVELAGAMGLDFVNYDGQ
                     HAPVTPETIDRFCRMCDGWGVTPTMRVPDQIPSNILNYIDRGIKAITIPFVNTRKEAE
                     AMVDCCYFAPTGHRSYTSKRVQRFGLVDDLKQMMQDTNDDLLLMPQIEDIKAYNNLDE
                     ILKVNEINVFAGGPNDLAQSMGFPGQPDHPDCIKVTDEGTEKIHAAGKFRNEDIMVSV
                     DATIAVRDSVREMLAGGSGIRGY"
     gene            complement(12440..12835)
                     /locus_tag="QF838_03300"
     CDS             complement(12440..12835)
                     /locus_tag="QF838_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013098.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJO59749.1"
                     /translation="MSYFINHVHIRSENPRTSAEWYEKFFGAKILSEKEVMPGTVTVS
                     LDAGGSTRLNISSQPDGSSRKTTPAELNVLGLEHFGFQTDDIQSDIENFEASGIPIVL
                     NITVIQGGTKLAYIEGPDKVLIELVQPAN"
     gene            complement(12913..13251)
                     /locus_tag="QF838_03305"
     CDS             complement(12913..13251)
                     /locus_tag="QF838_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59750.1"
                     /translation="MTIETLIVESNQTAKSKGWWDDPDRNIGEILALIHSEVSEALEV
                     YRLKGKDSLKENWFNEKGKPEGFTVELADVVIRIADLCGELGLDLESALTTKLSYNQS
                     RPYRHGNKKA"
     gene            complement(13278..14279)
                     /locus_tag="QF838_03310"
     CDS             complement(13278..14279)
                     /locus_tag="QF838_03310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59751.1"
                     /translation="MGKVGIMGLKAPGGSWEETKELAIMADDLGYSCITMGESWGEDA
                     FTSLAQIAAVTTQIKIGTSIVPVFARSPANLAMTALNMDVMSQGRFFLGLGASGKLVI
                     EDLHGEKFSKPITRIREYVDILRKAMKGERLDHTGEFFEISRFRLRFTPHRPDLPIYI
                     ASLSPPSLRMTGEIADGWLPIFLAPSRMDAAVAQLKQGAESANRTLNDITISPQVSIY
                     VTDDPKMARDQERPHISFYIGGMGVFYHQYMHRIGFGEEADMVRKAYMDKERSKAASL
                     VTDEMVDATTIIGTPEECAAQMGKFFDAGVDEIRLVLNEDTKKGYEETIQHLAPYIN"
     gene            complement(14325..15365)
                     /locus_tag="QF838_03315"
     CDS             complement(14325..15365)
                     /locus_tag="QF838_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59752.1"
                     /translation="MKIGIFDHVERLKSLTLHEQYNQRISMVKTAETLGFYSYHVAEH
                     HHSPLTAAPVQGAYLGALASSTSRIRLCPLVYVLPLHHPIRVIEEICTLDNLTEGRLD
                     IGFGRGAPVGDELAMWGQNPLESDSMYQESLQIIMQGLTREFVNFTGKHYDLQDLWME
                     ITPYQRPHPPIWVAGNPIKAGQIGANLITEGTIDNLPKINDLYSKTRSEHESIETVFH
                     FDENPLLGVSKRVLIGETDESALLRAKESFETYRSNFRKPLPDGTSRRPRIMIESEMG
                     KRILPWRVDFETAIEKEQIIAGSAESVIHYLERYKSNSGCNFLLLSFQWGDLNHEEAV
                     STMKVVGDEFIE"
     gene            complement(15386..15823)
                     /locus_tag="QF838_03320"
     CDS             complement(15386..15823)
                     /locus_tag="QF838_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HJO59753.1"
                     /translation="MSEYIPSPRDWVREQVELYESSSGTEGTTLRETGLPVIVVTNIG
                     NKTGSIRKTPLMKAVDGPNYILVASTGGAPKHPVWYFNLKANTSVKVQDGPNIYDMKV
                     REVTDSTERIRLWDIAVDAFPPYQEYQEKTERVIPVFLAEPVS"
     gene            complement(16018..16152)
                     /locus_tag="QF838_03325"
     CDS             complement(16018..16152)
                     /locus_tag="QF838_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59754.1"
                     /translation="MSLEIMGMIGTEKGNSSKSQVSVIGNGVSSEHIIHFAKAHENIC
                     "
     gene            complement(16168..>16899)
                     /locus_tag="QF838_03330"
     CDS             complement(16168..>16899)
                     /locus_tag="QF838_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO59755.1"
                     /translation="IKSVMNKQFTPLECDLSEPSSITKSAENLIRSGIQIDSIICNAG
                     IMALPKLQKKHGIELQFLTNHIGHFMLVNLLRPILTSRARITVVSSSAHQVFSYHDGI
                     QFDNFSGNRHYSPQKAYGHSKLANILFANELARRFQASGQTANAVHPGVIRTNLLRHY
                     NPVIKYGSIVLEKLLFKNVQQGAATQLYVSTHPDLKEVSGKYFSNCNLKETSKHGKDQ
                     AMAEKLWDCSEKIVNKISQGNDWEE"
BASE COUNT         4565 a         3425 c         3507 g         5402 t
ORIGIN      
        1 ccaattatcc agccgaatac atgacctcat ttatgaactc atatatggac aagaaggatc
       61 gtttgattgc ggctgtggcg gattgtcgta ggatgggtat tgagatatta cctccagata
      121 taaacagaag ctattcgaag tttacgattg aagagaacct ggaatctggg aaggcaataa
      181 gatttgggtt ggcagcaatt aaaaatatag ggtcagaagc attgagatca tttttggatt
      241 cacgagatca aacgggccct tatgaaacct tggcgcagtt atgtcatgat ggggatataa
      301 gttcattaaa tagaaaagcc attgagtgct tggtgatgtc ggggtccttc gacagcttcg
      361 gtgatcgtac cggcttattg gaagtaagtg atcgtattag ctccttagct caagatgagg
      421 ctaacatacg taattctaat cagtcgacaa tgtttgaaat gctgggagat tcagtaagta
      481 gtgctttgtc gtctatagat atacctttta catcaacctc agatcaccaa aaaaggctat
      541 gggaggtgga attgatgggt attagcattt ctggcgcagg taaccttggg aaactgctga
      601 gtggattcgg gaaagacgtc tctgttatgt tgacacaatt gcagggtgta agcccggcag
      661 ggttcacagt tttggcaggg caaatttcaa cagtggttga taggttcacg agagataata
      721 gaccgtttaa ggtggtaaat atagaagttt tagacggaag tttggaggcg gtggtatggg
      781 aagatgtgct tagtaaaact gcagaattgt gggagcccgg acggatagtt aaaatgaaag
      841 ggaatttgag agaaagagat ggagaagtga caattagcgt gactgaggca aatgaaatta
      901 atttagataa agctttcaac aatacaaatg atacgagtgc taatcccgaa gagaatgggg
      961 cgattttgca caccaactcc ccaatgataa atacaaatgg tgatggcaat cactctaatg
     1021 gatctgacgt acctaccaat agaaaaaaac tcattttatc tatacgggaa tcaaataaca
     1081 caaccaatga tcaaatgctg cttgatgata tcaagaggtt gcttctgagt gcttcaggta
     1141 atgatgaggt aggtcttgag atcgagacag aatcttccgt ggtagtgatg gagtggcccc
     1201 cagtgaaaat taatgcaacc ccggagttag agtcaaaact ttcagcctta gttggctcaa
     1261 caggtaaagt gagaattcag agcttaatgt tttgaaatat attgtttttc tagtttcagc
     1321 aactccaatt tcaggtcaag tctagtttta ccttttccaa agccagtcaa attaccattg
     1381 gcaccaatca ctctatggca aggaattatt aaagtgattt tgttgtttgc cattgcttga
     1441 cctaccgccc tggaggcctt tggactacca cttttatcag caacccattt ataggattta
     1501 gtagtgccga tctctataga cctacagata gcccatgctt tcttataaaa acgaggtgct
     1561 gtggatacgt ctattacaat atcttgaagt gaataatcct ggccttctag gtacccgtgt
     1621 agcttttcta tttctttaga aaacaaatcg cctgtttcta gatactctga tttccaccct
     1681 tttatttctt gaatacattt agattcttgt ctttctggca gcgaacaccg agttatacct
     1741 agttcacttc tctctagggc tatccaccct aaagaggtct cgaatttcgt gttataaagg
     1801 tgatgtggtt tcatttttct gagtccattt gaaagaacgt cctacaattt ctctccaaag
     1861 gtaatttcta attgtcttcc aacaggtatc caaattttcg gaataggaag attggggcga
     1921 atatttttcc ttcataaagg catatgcgat agtgtatatt tctgtttgga tactgggata
     1981 cttaattgac atttttgaag aaaattcact gatagtttgg tacttggttt ttgatgtacc
     2041 aaccaatgct ccaatcctgt acattttgat ggttagtttt tctgcttgcg acaagccaga
     2101 taatccccat tgccagaatg tattgaggcc gaaagacaaa agtacaatag aaaatataat
     2161 cgatatgggc actagaaacg attttttaag tataaaatat acagttcctc ttttattttc
     2221 actaacttca ctgtccgggc ctagttcgtc ataaaaatca tcaggtaatt tggggtaatt
     2281 cccatcctga tagacacttc ctgatcgaat aaaggccgaa ggagtttttt ctcgttggag
     2341 tcttgaatat ttactccagt taggggttgg ttcgaaatca atccatccat agttggggaa
     2401 atatacttgg acccacccat gactatcaga atctttgatg gtcgatgaat ttttatctgg
     2461 caaataagtc cccgaagaat agcctgtggc caatcgcgct ggaattccgg agattctaag
     2521 cataacagtc attgctgaag cgaaataatc actgtaaccg gtgagagtgg tgaatagaaa
     2581 gtgatctacc ccgtcagctc caataggggg tctcgaaatt ttatgtgaat attgataatc
     2641 tccgttgcga agatgctcct ctattgccac tgctttgtcg tagggatttg tttttcctga
     2701 tgtgatcttt gtagtcagat catatattct tttaggcata ttttctggta gctcaagata
     2761 atgatcctgg ataaagccgt ggtatttttg tccagcagtc ctgagttcat ttggcttaga
     2821 tacagataca gatgaaacca tcatgagctg cttgttagcc gggatttggc gagccagttt
     2881 ccaattgacg atatctaaat ttaacggcac cgttcgagtc aattttattt tctctgtgcc
     2941 attattgttt gctgaagtgt ttacatattt gtattcatcg ggaagttggg ataacagatg
     3001 ttcttcaaat ttattattgc ggatatcagt tggatattct tctaaaaact tttctatatg
     3061 cgactcaaat tgttgcaagt cagaaggtag atacccatcg aatttgccat ctccaacttc
     3121 gatttcgaag gtcttgggtt tgagagattc caggactatt ggttcactca cactagaaat
     3181 attgcctccg gcaaagaact ggtccgatgg gaatgacatc tctatacttt gactgacgga
     3241 ctttgttgat tgtaagcccg ttgtattgtc gaatagagaa tatttatcta gatcgatggt
     3301 atgagtttca ccagatatcc agccttcatg cgtgtacgta ttatacgtgc gtgaaaccca
     3361 ataattagga taatctgatt tggctgttag taccccttgt tctcgaaatg aaatcgatcc
     3421 aataaaaggt agaaatttgc caaaaaacct accgttttgt tgtgtccttg aggggacact
     3481 accgagaagg cgagcgaatt cattttcaat catcgcaaca ggttctctcg ctgctttcca
     3541 ggtgtcggca acaggtcttg aaacatatat ttttagcggg attgcagtag caataatcag
     3601 tattgctatg ctgtaccaaa ttgcagaatt taaagttagc cacccgtcca attgattgaa
     3661 gactgttcca tttttagacc actcacctct ttgtttcacg gttgttatat gtgttattaa
     3721 aatcatagcc gtgaaagcat agagatagaa cctggaactg tactgctcag gtaagaaact
     3781 taagcaggtt aaaatagaca ttccgttaag gacgatcgat atccaaatgt tgttatgcct
     3841 aaaggcaaac caggaggagg tataccccaa tacccaagcc aaacttagaa ggaaaatggt
     3901 gtagggaatc aagtcagtgc ttatgccttc agtgctagct attccatacc aataatccag
     3961 acgtaatttt agttcagaaa ctttttcgtt tacgggtaaa tcttttatga gcgtgagcgt
     4021 gttatagagt acaaggattg ttccaatcga aaatgaaatc agatggatca atagtggatg
     4081 taactttatt cgatgaaaga ataggggaaa taggcttgaa aggagaacca tggagtagat
     4141 acctggggtt tctacccaat tccctatcac tactatatcg gcagtgcaga tgagagaaag
     4201 taaaagtatt acgtataatg ttataccttg ttctggtata aatgttttca aattggatat
     4261 tgaattcgcc catttcgttg ggggtgcttg tccaggtttt aataactcag aaatacgtac
     4321 ttctttaatc atgatgactt tgtcatgctt aaattttgta ccgatctctt aaaaaaagat
     4381 cgtgataaag attcattaag ctcatcattt ttatttatta cgtacggaga tataccaata
     4441 ttgtctagcg ctgacaatat tggccctaat tcatgcttgc caccgaatga ctttgcatta
     4501 accatgatta cagtgatact tgtattaaat tgagacaatt cagataaggc cgtgacccat
     4561 tctgtttctg aagacggggt tatgatgata atggatgaat tattgaacca tatgtttctt
     4621 tcttcagcta tcacctgctg gagtttttta ccgtttccgg gctttgctac tgctaaggtc
     4681 tgcattgtac ggtcaaaatg agaggaccct atcccgggtg ttatgtagta ccgatcattt
     4741 ccatgactta gaaacccgac aggagtatga gagtcagtgt agcttttagt aagggaggca
     4801 gtcaccgaaa tcgataattc atcggtactg tcgtccaatc tgccgcactg cacagattta
     4861 tctagatcgt tcagaattac aacttcattg gatgaaccga catcaaattc cttggacatt
     4921 agccggttgg ctcttgctgt ggttttccaa tgcactctgc ttaaactatc tccataagcg
     4981 tattccctga cactggaggc gtgtggagag agaatgttgg atttttttct tgatgttcct
     5041 tcatttgtta tctgagatgt cccaatattg aaatttctca aatcgtatat tttgggataa
     5101 accattattt tatcgatctc gccgtggcga gttgatgctt tatatattcc tagtaaatcg
     5161 gttgttgaga tctctattgg tcccatttca aacacgcctc tttgtcgtgc ataatcggta
     5221 gatctccagg atctgtaacc tttggcggtt aatcctagtg caaaggaact tgtgtactca
     5281 ggaattgtgg tgatgtcagt agcaagtatt aacgttttgg gtattacact taggtttcta
     5341 atacttattc ttttatctat ttgttcaccg acactaagca aatggtttcg gcgatcgata
     5401 ctgacttcaa tttttcttat atttaagtaa actgaaatca aagacaccag gctaacgata
     5461 attatgatgt acgctagtcg aaagaaaagc tcaaatcctg tggccagtgc ggtgatcaca
     5521 gtgataatta ataacaccat gattaggtaa aatctcggtg taggtgcaaa tcgaatattt
     5581 gagatgagtt tatctcctga accagctctg agatctttca ccaggtatgg atgtggattt
     5641 cataagatct tgaatgactt gttctggttc catgccgttc attctggctg aaggggaagg
     5701 tataagtctg tgggccaaaa tggatggcgc taaatgtttt acatcatcag gtaatacgta
     5761 atctctctca cgtataatgg ccattgcctg acttgcccga tgcaatgcaa tagatcctct
     5821 tggtgaactc cctaggctta gttctagcac atttcgagtt tggctgacta ttttcacaat
     5881 gtactctttg atcagatcgt ctacatagac tgatttgacg gcctcctgca tgtctgtgac
     5941 catttttgga gttacaacag aggaaatgcc gtcaataggg tgagtcaatg attgcttgct
     6001 taagatcgca atttcatcta cttcatttgg gtaacccaaa ttgaccttta gtaagaatct
     6061 atcaagctgg gcctcaggta aggggaaagt gccttcatat tcaattgggt tttgggtggc
     6121 gataaccata aagggtttgg gaagttctct ggtaataccc tccatagtta cttggttttc
     6181 ctccatggct tccaacatag cagactgagt tttaggtgtg gcacggttaa tttcatccgc
     6241 caatactatt tgtgaaacta taggtccttc cctaaattca aaatcgccac ttttttggtt
     6301 gtatattgat actccgctga cgtctgttgg taataaatca ggggtaaatt gaattctctt
     6361 aaaagtgcat ccacttgata tagcgaggct ccgggccaaa acagttttcc caacccctgg
     6421 tacgtcttct actaaaatat ggcccccact catcaaggca attgtggcca actctacaga
     6481 agtttgctta ccaactataa cgtggttgat attatcagtg ataagatttg ccgttttggc
     6541 cgcttcatta catgagtgat ttgtcttttc tgtcacaatt tatcctttcc tttaatatgc
     6601 gatcattccc aaataatgat cgcatattaa gtgcctctag catactggta taaaggtgct
     6661 gcaatataat ataaacgata atgaattgag cctacgtgag agtccctgca atttagtgaa
     6721 ggagtgaaac aaatgaaagg attcttgagg ggtctggtat ttggaggcct agcactttct
     6781 ttgtactggc ttgtgttcag cctatgtgag ccttaaataa tcgggttacc ctaatggtgg
     6841 accgataaat tgcactttgt atttgtctgc tagctgaagg gcccgctccc cgaaggtctg
     6901 tccgtcaatt tcttcttctc cgacagcctc aaagaaaaaa ttttcgaatt gaggtgggga
     6961 aataatcact agaatcttag taggttgctc atttgctttc cagaatgtgt ggacaatatt
     7021 tcgtggaagg aaaacaaatg atccagcttg ggctacaaaa gtgtcatctc ccaatttgat
     7081 gttaatttca ccttctagga cataaagggc ctcatcttca ttcttatgga tatgttggcc
     7141 tgggccgccg ccgactaagt tggcttcaaa cagagaatac gccccattcg tgtcttcttt
     7201 ggttgcctta aaagttaaag ggtgatcaga cacctgcagt agtttgccct catttgggcc
     7261 taaccaattc tattgtcggt aaatgacatg gaattctccg aaatgatttt tggttaggct
     7321 ctttagacat actgtttttg ttaatggtgg gcgatgaggg actcgaaccc ccgactccct
     7381 gcgtgtaaag caggtgctct aaccaactga gctaatcgcc cttaacgcag agcgcgcttg
     7441 gcgggacttg aacccacgac ctcagccttc gcagggctgc gctctatcca actgagctac
     7501 aagcgcattt gaaagtatta cgctccagat taggagtatt aaaaaagaaa ctcttttaat
     7561 ctgtagaata aatgttaatt ccacaaatag cactagtcaa tctcgattgg gattatattg
     7621 aaatttgatt actggtatta ctctatcagt tttgttctga tatttttgaa aattgggata
     7681 aatatctatc agtgattccc atgctatagc tttgagatcg tcctcgagta tgacagcagt
     7741 agcattcaag aatttgcctt ctgccagaat ttcaacatct gggttagaga aaatatttcg
     7801 gtaccactga ggagctttag gactcccccc aaaggagccg gcacagtaat aatgaccgtc
     7861 aatttttttg aacagtaacg ttgtgtagcg tttcttccct gtctttcttc ccactgtcgt
     7921 aagcaggagc atatctaacc ctgccaacct ctccataaat tttcctctag tcaaacgaag
     7981 aattaaggaa tggaatttgc tgaatgcgac gataagtgtt gattgaagat tcatctaata
     8041 taagttcaat cgaaattctt caaattcttt attttttcaa gatttagttg caggatgcat
     8101 tgcccaacaa ggcctatggc tttctccaca tcttttatac ttattataga attgtgacta
     8161 tgtagatatc ttgtggggac agtgatattt attgcaggag cgccctcaaa agatttttgc
     8221 atctccgctc catcttgccc atagccactc aaaacgttaa attgtaaatt tatgtcggaa
     8281 tctttggcag tatctatcac gaaatcacgg agtttgaggt tcggaagcat agatgaatcg
     8341 tgtaaaaaga ttgctggccc attcccaagt ttctcctgag cctcgtactg tgaaatactt
     8401 ggatagtcgc cggccactcc ggattccaaa tttaagccta tatcagggtt tacagcatag
     8461 ctgcttgtat gtgccccacg taaccctatt tcttcttgca cagttgccac cgcacatata
     8521 ttgaaaggca aatcgctgtc tttgactgac tccattactc ctactaaaac agcaaggccg
     8581 gccctgtcgt cccatgcttt acctaatatg aattcactat cgtttagtct tgtagcatca
     8641 ctaacaggtg ctataggatc tccaggtttt atccctaatc tgttctcggc atctgcttta
     8701 ttttgagcac caacatccaa gaataggttt tctcctttaa aaacttttcc cctttcatct
     8761 gtcgacatca catgtggggt tttgattcct gttataccta aaacagtccg ttttgatgtc
     8821 agtatttgcc atctctgacc aattattgct tgatctaacc agcctcccaa gttctgaaat
     8881 ttgataaacc cttcatcagt tataaacctc acaagcaatc cgacttcgtc catgtgagca
     8941 gagagcatta tattgagaga gctagatgac ccatttttta taccaataag tgaacccatt
     9001 ccatccgtat tcaaagaatc tacgaatgga tataattctc ttttcattat ctctcgcact
     9061 ggattttcga accccgaagg tcccggagca tttgagagtt ctagcagtaa tgattctaga
     9121 ttaatcatgg attcccctat gtaaagtcag aatgttcgac cagcatctgt tttaagtcat
     9181 aagtatagtt aaatattctt ctaaggtact acaattatat tttgtagtgg tatcttctct
     9241 gccaaattgg gtattattgc ttgggattaa attaataatg actccactat cgtttgtgtt
     9301 ttaaaggttt tatggttgag ataaagactg attcaatgcc aaacaggaag ttttccttcc
     9361 tcgatgttgc caaagagttt gtccctaaaa ttcttgatgc ttcccgggta atttctgaag
     9421 gtagagaaat acctccccag ctggcatcag aaatggcgga agagggactt tttaggcttt
     9481 atattcccaa atctgttggg ggctatgaaa tagattacct agagttttta gagttggttt
     9541 cagtcattgc agaggccgat ggaagcgtcg gctggtgttt taatcaaaat aacgtactgt
     9601 caaccttatc cgcttttatg ccgaaggagt tagctcagga aatttggtct gacccgactg
     9661 ttgttttgtc taatggacca ccgatttccc ctgtcgccgt tccagtggaa ggcggatacc
     9721 aattgaatgg tagatggaat ttcagttccg gttcgcgtca cgcaaaatgg gtggtggctt
     9781 tagctcctgt taaaggtagg gtgctggagg atggtgaatc tcctgagatg cgaaatatga
     9841 tgattccaaa ggaagaggtg gaatttatcg atacttggga agttaatgga ttgagaggaa
     9901 ctgggagttt tagtttcact gctaaagatt tatttgtgcc ggaatcccat tcatttgtgg
     9961 aaggtggacc ccctagggaa gaagggccaa tatatttgat tcctaagagt cttttgtttt
    10021 gctcgggttt tgcgaccacc gcattgggtg ttgctagagg ggctttaaat gctgtaattg
    10081 aattatcatc tgccaagact cctcaagaac aagatttact aatgaagcag ccttttacac
    10141 aaagagaaat tggacaagca gaggctatat ggagatctgc taacgcgtat cttcgggata
    10201 gttctgaagc tgtttgggaa agtgcggtta atgatagggt gattcctgtg gagacgagaa
    10261 taaatttgag actttccagt acccatgcga ttagagaggc ggtaaaagtg gcagacatgg
    10321 catattccct tagtggggca accgctattt ttgaaaccag tccaatacaa aggcgtatgc
    10381 aggacgttag ggtaatatct caacagattc aaggcagaat gtcccactat gacacagcag
    10441 gacaatattt tcttgggatg gacccgaaaa gtaggttgtt ttaggttttg tccgagagta
    10501 atttctttgg ttttattatt ccgaatgaac ttgtgtatcc gtggacgtag gtagattccc
    10561 ccaccggacc gacttctcca ttgcagaaaa aaccggttaa gggtatttca ccaacaacct
    10621 ccttaaacat gttggtgtcg tgattagctt caccatatag gtatgcacct cttccgaggc
    10681 atgaaaacag cagggctccg aattgacttt gttcagactg gtcactgttg tagttctcta
    10741 acattatctt tagattttct gaggaagtat ttgagtcgcg tagatgaaat tgtactaatt
    10801 ggccttctct tagcatttct cctattgcta attttccttc atcggggttg gcaccgacta
    10861 tattacggat caaaaagtct ttcgtggtag gggggtcaat aaaaggatcg gtaacggtgc
    10921 ctaaaaacaa tgaattctgg aataattgtt gatccggttc agaaagttga ttgtatattt
    10981 ctgacaaaac tgtaaaaggg ttatggtcat ctaatccggt cagtatattt cgttcgcatc
    11041 cggttatcct cataaggctt cctattggtt tgcaaccctg ggcaacaata gtgtctattt
    11101 ctacgtcgcc agctagagct ataccagcca caccttggtc agtggttccg tttaaatata
    11161 tggcgttact tctcggttgg gaccctccac tagctatacc accgatgatt gtggagtctg
    11221 gaaaggcata gtccattccc ttgataagac tttgtatttc tactgtaaaa ggatcagcaa
    11281 gaataataaa tttcggttct gaatctcttt taacctttag tagagattcc catttttcag
    11341 gtggttgatc accatccggc aattcagtgc cagtaatatg aaatggggta atcgaaacat
    11401 caggcatcat ggcagccgtc agcgctattt ctggcctatt ctcaacttca atggcttctc
    11461 ctatcacgcc attaccagag cagcctatga gcgtagtttc aggaaaatat tcagaaacta
    11521 aaccgggtaa atcttggtac aaggcttgaa aatctggaga tataaatgct atgagtaggt
    11581 gaggttgagc ctctaaatcc gcttcaattg cagacagaca ttcacgtacg ccagtttcaa
    11641 aattttcgga ctctgatatt tttgaagacc aatccatatt tagtaccctc tgattccact
    11701 cccacctgcc agcatttctc taacagagtc acgaacggca atggtggcat ctactgatac
    11761 cattatgtct tcatttcgaa acttgcctgc agcatgtatt ttttcagttc cttcatcggt
    11821 gaccttaatg cagtcagggt ggtcaggttg acctgggaat cccatggatt gggcaagatc
    11881 gtttggtcca ccggcaaata catttatttc attcaccttc aatatttcat ccaaattgtt
    11941 gtaggcctta atatcttcta tttgcggcat gagtaaaaga tcgtcatttg tatcttgcat
    12001 catttgcttc aggtcatcta ccagtccaaa tctctgaacc cttttgctgg tgtaacttcg
    12061 atggcccgtc ggggcaaaat agcagcaatc caccatcgct tccgcttcct tacgggtgtt
    12121 tacaaaggga atagttatgg cttttatccc gcggtcaatg taattcaata tgttgctagg
    12181 tatttggtct ggaacacgca tagtgggagt tactccccat ccgtcacaca ttctacagaa
    12241 cctgtctatc gtttctggtg tgaccggagc atgttgcccg tcatagttca caaaatccag
    12301 acccatagcc ccggcaagtt ccaccatttc atcagcataa aaacttaaag atatgccata
    12361 ggatttctta ccttctttca tgttcgaaat aattttgtta ctcattgtta tttactcctt
    12421 tgctcattta tcaagctgat taatttgcag gttggaccag ttcaattaat actttgtcgg
    12481 gcccttcaat gtaggctagc tttgtgccgc cctgtatcac agttatattt aaaacgattg
    12541 ggattcctga cgcctcaaaa ttttctatgt cggattggat gtcatcagtc tggaacccaa
    12601 aatgttcaag tccaagtaca tttaattctg caggggtggt ttttcgggat gaaccatcag
    12661 gttgagagga tatatttaat ctggttgaac ctccggcatc caggcttaca gtaacggttc
    12721 caggcatcac ctctttttct gaaagaattt tggcgccaaa gaatttttca taccactcgg
    12781 ctgaagtcct gggattttca gatctgatat gaacgtgatt tataaaataa gacatgtata
    12841 ttcgatcctc tatattgttg gcatatttga tcccaaaaat ctgacttagt aaacatgtga
    12901 cgagtaatcc aattaggctt ttttgttgcc atgacgataa ggccttgatt ggttatagga
    12961 taactttgta gtcaaggcgc tctctagatc taaaccaagc tccccacata aatctgctat
    13021 tctgataact acatcagcta attccacagt aaatccctct ggtttacctt tttcgttgaa
    13081 ccaattttcc tttaaggaat cttttccttt taatctatat acttcaagag cttcgctaac
    13141 ttcactgtgg ataagagcaa gtatctcccc tatatttcta tcaggatcat cccaccatcc
    13201 cttagattta gccgtctgat tagactctac tatgagggtt tcaatcgtca tggacatttt
    13261 ttggtcctcc catgcaacta gttgatgtat ggggccaggt gctggatagt ttcttcataa
    13321 cctttcttgg tatcttcatt gagtaccaat cttatttcat cgacaccagc atcaaaaaat
    13381 tttcccattt gagctgcgca ttcttccgga gtaccgatta tagtggttgc atcaaccatt
    13441 tcatccgtga caagactggc agctttgcta cgttccttat ccatgtaggc ttttcttacc
    13501 atatctgcct cttccccgaa cccaattctg tgcatatatt ggtggtaaaa aactcccata
    13561 cccccgatgt aaaaagagat gtgtgggcgc tcttgatccc tcgccatctt tggatcatcg
    13621 gtcacataaa tagatacttg gggtgaaata gtaatgtcat ttagagtcct atttgcagac
    13681 tcggcccctt gcttcaattg agcaactgca gcgtccattc gtgatggggc gaggaatata
    13741 ggaagccatc catcagctat ttcacctgtc attctcaagc taggagggct tagcgaagca
    13801 atgtaaatcg gcaggtcagg cctgtgagga gtgaatctca atctgaatct acttatctca
    13861 aaaaattcac cagtgtgatc taacctctcg cctttcattg cttttcgaag tatgtctaca
    13921 tattctcgga ttcttgttat cggttttgaa aatttttcgc cgtgcaagtc ttcaataacc
    13981 aatttaccac tcgcacctag cccaagaaaa aatctgcctt gtgacataac gtccatgttt
    14041 agggccgtca ttgcaagatt cgcgggtgac cttgcgaaga ctgggacgat gcttgtacct
    14101 attttgatct gagttgtaac agcggctatt tgggctagtg aggtaaatgc atcttcaccc
    14161 catgattccc ccatcgttat acagctatat cctagatcgt cagccataat agccaattct
    14221 ttggtctctt cccaagaacc cccaggagct ttgaggccca ttattccgac cttacccata
    14281 actttctcct ttctgttgat gtgaatttat tctatccgtt attttcattc tatgaattca
    14341 tcacctacta ctttcattgt gctaacagct tcctcatggt ttaggtcccc ccattgaaag
    14401 gaaagaagca aaaaattgca tccactgtta cttttgtacc tttctaaata atgaataacc
    14461 gattcagccg atccggcgat tatttgttct ttttcaatag cagtttcaaa atcgactctc
    14521 caaggtagta ttctttttcc catttcactt tctatcatta tcctaggcct ccgactagtc
    14581 ccgtcaggga gcggtttacg gaagttggag cggtatgttt caaacgattc tttagctctt
    14641 aataaagcag attcgtccgt ttctcctatt aatacacgtt tggaaactcc caagaggggg
    14701 ttttcatcaa aatggaacac cgtttcaata gattcatgtt cagagcgggt tttcgaataa
    14761 aggtcattga tttttgggag attatcaatt gtgccttccg ttattaaatt tgcacctatc
    14821 tgtccagcct tgataggatt tccagcaacc caaataggag gatgtggccg ctggtaggga
    14881 gttatttcca tccaaagatc ttgcagatca taatgttttc cagtgaaatt gacgaattct
    14941 ctagttagac cctgcattat gatctgcaag gattcttgat acatactgtc tgattcaagc
    15001 gggttttgtc cccacattgc cagttcgtct ccaaccggag cgcctctacc aaaaccgatg
    15061 tctaatcttc cctctgtgag gttgtctaag gtacatattt cttcaatgac tctgataggg
    15121 tgatgcaggg gaagtacgta cactaatggg catagccgaa ttcttgatgt gcttgaggcc
    15181 aaggcgccaa gatatgctcc ttgaactggg gctgcggtta atggtgaatg atgatgttcg
    15241 gctacatggt acgaataaaa ccccaaggtt tcggcagttt tgaccataga tattcgttga
    15301 ttatattgct catgcagggt tagggatttg aggcgttcga cgtggtcaaa aataccgatt
    15361 ttcaaggact ctactcttta atagcctagg aaacaggctc agccaaaaaa acaggtatta
    15421 cacgttcggt tttttcttgg tattcctgat atgggggaaa tgcatccacg gctatgtccc
    15481 aaagccgaat tctctctgta gaatcagtta cctctcttac cttcatgtca taaatatttg
    15541 ggccatcttg aactttaact gaggtgttgg ccttcaaatt aaagtaccaa acaggatgtt
    15601 taggagctcc tcctgttgat gcaaccaata tgtaattagg cccatctact gctttcataa
    15661 gaggagtttt tcttatagaa cctgttttat taccaatatt cgttactaca atgaccggca
    15721 gaccagtttc ccgaagggta gtaccttccg ttccactgct cgattcatat aactccacct
    15781 gctctctcac ccaatctctc gggctcggta tgtattcact cattttatta gttcctttca
    15841 aataagctta tagttggcat atttacccag ctgccaatgc tgagaccacg tttgctttat
    15901 gatattttct cactttatct ctgagcaatg gtattaactc gttgccccag tatatcgcgt
    15961 cttcaactgg attaaatccc ctaatcaata tagtagtaca acctatcctg taatatttca
    16021 acaaatgttt tcatgggctt tcgcgaaatg gattatatgt tcgctgctta ctccgttacc
    16081 aataacactg acttggcttt tactggaatt acctttttcc gtgcctatca ttcccatgat
    16141 ctctaaactc atcgtctttt ccttatatta ctcttcccaa tcatttcctt gagatatctt
    16201 atttacgatt ttttctgagc agtcccataa tttttctgcc attgcttgat cttttccgtg
    16261 tttggaagtt tcctttagat tgcagtttga aaaatatttt ccgcttactt ctttgagatc
    16321 cggatgggtg ctgacatata gttgagtagc ggctccctgc tgaacatttt tgaaaagaag
    16381 tttttctaat acgattgacc cgtatttgat gacagggttg taatgtctta gcagattagt
    16441 cctgataacg cctggatgga ccgcgttggc tgtctgtccg gatgcctgaa atcgcctggc
    16501 cagctcattt gcgaacaaga tatttgctag tttggagtga ccgtaagctt tttgagggct
    16561 gtaatgccta ttgccagaga agttatcaaa ctgaatccca tcgtgatagg aaaacacctg
    16621 gtgtgcagag cttgatacaa cagttatgcg tgctctgctg gttagaatcg gtcttaatag
    16681 gttgactagc ataaaatgac caatatgatt tgttaaaaat tgtagttcta tgccatgttt
    16741 tttttgcaac ttgggcagtg ccataattcc agcattgcaa attatagaat ctatttggat
    16801 gcctgaccta atcaaatttt cagcagattt ggtgattgaa ctaggttctg atagatcgca
    16861 ttccagaggt gtgaattgct tgttcatgac tgattttat
//
LOCUS       DASCNS010000090         4440 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000752615, whole genome shotgun sequence.
ACCESSION   DASCNS010000090 DASCNS010000000
VERSION     DASCNS010000090.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000752615"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="QF838_03335"
     CDS             complement(<1..423)
                     /locus_tag="QF838_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59756.1"
                     /translation="MVIQTTNPFAPAKPRVPLIPRRLQPVPDPIEIAKHNKTHCPKCS
                     SLLRVNYQDAECLSCGFVDYDYVAPEQEKKGNTLIGTGTRYVIRYMGEFDALSDVVTH
                     VKVYRVRNRVMYGVSCPFCGSGMAQSSLSGKRREVREER"
     gene            852..1652
                     /locus_tag="QF838_03340"
     CDS             852..1652
                     /locus_tag="QF838_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HJO59757.1"
                     /translation="MSLNIIVCAKQVMDPETPLSAFNVDEAAKRVVPAQGIPPVVNGF
                     CENAVEAALKIKEAAGDATITVISVGTDFVMDVMKKPLSMGADQMILIQDPVAENLDA
                     LGTATVLSKAIEKIGEADIVICGQQASDWDNAHVPLGVSEMLGVACLSEARSIELGDG
                     TVTVASSRSDGYEVFEAPLPCLITVNNEIGEPRYPTLRGIMQASRKTPETWTLSDLDL
                     SESDVSPALTLSELFVPASDRQCEIIEGEDEADSGRLLALKLREEKII"
     gene            1691..2680
                     /locus_tag="QF838_03345"
     CDS             1691..2680
                     /locus_tag="QF838_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HJO59758.1"
                     /translation="MSEVIIFAEIDGDDLHSTAGELISAGKKIAADTGSLVSAALIGG
                     NQGLASQAISLGADKVYLANDDAIQEGVADAYLAVLHKICIATEPTVVLTSKTPIGRA
                     VGPRLAYRLGSGIAQDCTDVSADSDSRRVTATRPVYGGNAMAKFTFADNDPQVVIMRL
                     KAFEAAAPDSSRTGEVIEFEVSLDESDIKSKLVETVKEESEGVRLEDASVIIGMGRGL
                     GGPEPFSQLEELAKVFNGAVGASRAVCDAGWLDHSFQIGLTGKTVTPDVYITVGISGA
                     SQHMAGCSSSKNIVAINKDGDANIFKEASFGVVGDWEKVLPAFTATARELLES"
     gene            2730..3170
                     /locus_tag="QF838_03350"
     CDS             2730..3170
                     /locus_tag="QF838_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59759.1"
                     /translation="MNGNNFQDDIEEIKNTLLEADVVSLFFPYFGKTVLIDIRSNETD
                     GPALILTEMVRNPRERIRSMGQLRPGFNDPEKMVLIPWVRYLETLIDSGIWSIVIKKL
                     EEASFIDPKASADEILKKLRNLEHSELADAVNGPSYKTIWSKPG"
     gene            3228..3881
                     /gene="npdG"
                     /locus_tag="QF838_03355"
     CDS             3228..3881
                     /gene="npdG"
                     /locus_tag="QF838_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HJO59760.1"
                     /translation="MLGFIGGTGPEGKGLALRFAISGLSVFIGSREKQRGIEAAQEIR
                     SQFTEIGSDMVAGGTNEEAAIASDIAVICTPYAGHKATLENLKHVLNGKIVVDVVAPL
                     SFEKGSASAITVEEGSAAEQAQSLLPDSLVVGAFQNASAEDLLMPSQDMECDVIVCSN
                     DQSAKEQVMLLGERIPGVRSIDGGNLQNAKYVEQLTALLININKIYRAHSSIKIVGI"
     gene            3917..>4440
                     /locus_tag="QF838_03360"
     CDS             3917..>4440
                     /locus_tag="QF838_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59761.1"
                     /translation="MRLITCVMLTGLCCFFWFNLTVLLASTPDDWFIKPSIYHQIEYP
                     DNVQFNLTGFSNEDLLEVRLKYSYGNSKIISYKYIDPPSKGEIRSEVTLNTSGPNYIP
                     AGTQITYFFDLTSVKGKKYQSPQIKFHYLDPSIKWKSTELNHLTLIWHDRNESDINSV
                     IEDVNKSNLRFTKL"
BASE COUNT         1399 a          859 c          957 g         1225 t
ORIGIN      
        1 tctctcttcc ctaacttccc tacgtttacc tgaaagtgaa gattgagcca tgccagaacc
       61 acaaaacggg caactgacac cgtacatgac tctgttccta acccggtaaa ccttaacatg
      121 ggtaacaacg tcactgagag catcaaactc acccatatag cggatcacgt accgagtacc
      181 ggtaccaatt aaagtattcc cttttttctc ttgttcagga gcaacataat cgtagtctac
      241 aaacccgcag ctaaggcatt ctgcatcctg ataatttaca cgcaataacg aggaacattt
      301 aggacagtgg gtcttattgt gttttgctat ttcaatagga tctggtactg gctgcaatct
      361 cctcggtata agagggactc ttggttttgc aggagcaaaa gggttagttg tttgtatcac
      421 catatttaat acctctcgtc accatgtatt tctaaaaatt aatacaggcc actatttaat
      481 taagtcttca gatttttgtt aaaacatgca gagggattca tctatttcaa catttttcta
      541 aattaatgct aggcattttc ttatacttgt ataaaaaaag tcaagtgttt gtatctaaat
      601 ttttgtttaa ctttgtatta atttggtcat tcttggcatc atattagaaa aacttcttta
      661 atttaattct ttattcttcc tcattatttc tacaaacaag aaatttttga aagagggtga
      721 aattcgatat accactagaa ttgacgccta ttatcaagcg ttgctatatt gggcaagctt
      781 ggctctttta ataaaggcta cgtataatta aactagctaa gtaatttgac tttaataatt
      841 aggagtaagc catgtcttta aatatcattg tctgtgccaa acaggtaatg gatccagaaa
      901 ctccactgtc tgcctttaat gttgatgagg cagctaaaag agtcgtccca gcccaaggca
      961 ttccacctgt cgttaacggg ttctgtgaaa acgcagtaga ggccgctttg aaaataaaag
     1021 aggccgctgg ggatgccact attacagtaa tatccgtagg aaccgatttc gtgatggatg
     1081 ttatgaaaaa acccctatct atgggggctg accagatgat tctaattcaa gatcctgttg
     1141 ctgaaaatct ggatgcatta ggtaccgcaa ctgtactttc aaaagctatt gagaagatag
     1201 gagaggcaga tattgttatt tgtggacaac aagcatcaga ttgggacaat gctcacgtac
     1261 ctctaggggt atcagaaatg ctaggagtag cgtgcctatc agaagccaga agcatagaat
     1321 tgggagacgg aacagtcacg gttgcgagtt cacgttccga cggatatgaa gtgtttgagg
     1381 ctcctttgcc ctgtcttatc accgtcaata atgaaatagg ggaaccgaga tacccgacct
     1441 tacgggggat catgcaagca agcagaaaga ctcccgaaac atggactttg agtgatctgg
     1501 atctatcgga gtcagatgta tcacctgctc tgaccctatc tgaactattc gtacctgcta
     1561 gcgatcggca atgtgaaatc atagaaggcg aagatgaagc cgactcagga aggttgctag
     1621 cacttaaact acgagaagag aaaataatat aaccatcagc atttttcact attaacatgg
     1681 agtttctata atgtcagaag taatcatatt tgccgaaata gacggggacg accttcactc
     1741 aacggctggc gaactgatat cagcgggaaa gaaaatagcg gctgatacag gaagtcttgt
     1801 ctccgcagct ttaattggag gcaatcaagg tttagcttct caggcgattt cattgggtgc
     1861 tgataaagta tacttggcca acgatgatgc tattcaggaa ggtgtggcag atgcctacct
     1921 ggcagttcta cacaaaatat gcattgctac cgaacctaca gtggttctca cctcaaaaac
     1981 cccaattgga cgggcagtcg ggccaagatt ggcatatagg cttggatccg gaatcgccca
     2041 agactgtacg gatgtttcgg cagacagtga ttcaagaaga gttactgcca ctagaccggt
     2101 ctatggtgga aatgccatgg ctaagtttac ttttgcagac aatgatcccc aagtggtcat
     2161 tatgagacta aaggcgtttg aagccgctgc gcccgactct tcgaggaccg gagaggtgat
     2221 cgaatttgaa gtttcgcttg atgaatctga tattaaatct aaactggtcg aaactgtgaa
     2281 ggaagaatct gaaggggttc gccttgaaga tgcatcagtg ataataggaa tgggacgtgg
     2341 gctaggtggt ccagaaccgt tctcgcaact tgaagaacta gcgaaagttt tcaacggagc
     2401 cgtgggagcg tctagggcag tatgcgatgc gggttggtta gaccactcct tccaaatcgg
     2461 actaacagga aaaacggtta cccccgacgt ctacataaca gttggcatat ctggagctag
     2521 tcagcatatg gccggttgct cctcgtccaa aaatatagtt gcaataaata aagacggtga
     2581 tgcaaatatt tttaaagaag caagctttgg agtggtggga gattgggaaa aagtattacc
     2641 agcttttact gcaacggcaa gagaactact tgagtcttag caacaatggg cagttgagtc
     2701 tatttgaatc ttaaacttgg caaaaacttg tgaatggaaa taattttcaa gacgatatcg
     2761 aagaaataaa aaacaccctc cttgaggctg atgtagtgtc gctatttttt ccttacttcg
     2821 ggaaaactgt ccttatcgat atccgttcaa atgaaaccga cggaccagcc ctaattttga
     2881 cggaaatggt cagaaaccca agggaaagga ttcgaagcat gggacagcta agaccaggat
     2941 ttaacgatcc tgaaaaaatg gttttaattc catgggtaag atacctagaa acactgatcg
     3001 atagtggaat ctggagcata gttattaaga aattagaaga ggcaagcttc attgatccaa
     3061 aagcttcagc tgatgagatc ttaaaaaaac tccgaaatct ggaacacagc gaacttgcag
     3121 acgcagtaaa tggcccatcg tacaaaacaa tatggtccaa acccggataa agataaacct
     3181 caaatatgtt ggtcattttg ataaatttca tagagacagg tgactagatg ctaggattta
     3241 taggaggaac gggaccggaa ggaaaaggtc tagcattacg tttcgccatt agtgggctca
     3301 gtgttttcat aggatcaaga gaaaaacaaa gaggtattga ggctgcacag gaaattcgtt
     3361 cacaattcac ggaaataggc agtgacatgg tggcaggtgg caccaatgaa gaagctgcaa
     3421 ttgcttcaga tatagcagtt atatgtactc catatgctgg ccacaaagct acattggaaa
     3481 acttgaaaca tgtcctgaat ggaaaaattg tagtggacgt agtggccccg ttgtcatttg
     3541 aaaaaggatc tgcttcagca ataactgtag aagaaggatc agcggcagaa caagctcagt
     3601 cgctattacc cgactcctta gttgttggag ctttccaaaa cgcgagtgct gaagatcttc
     3661 taatgccttc tcaagatatg gaatgtgacg ttatcgtatg ttcaaacgat cagtctgcaa
     3721 aagaacaagt catgcttctt ggagaacgca ttccaggtgt cagaagtata gatggcggca
     3781 acttacaaaa tgctaaatac gtcgaacaac ttaccgcatt attaattaat ataaataaaa
     3841 tttatagagc ccattcctcg ataaaaattg ttggtatata ggaataaaaa acttcgttaa
     3901 cctcactagg aattttttga gattaattac ttgtgtaatg ctaacgggtt tgtgctgctt
     3961 cttttggttc aacctaaccg tattacttgc ctcaacacct gatgactggt ttatcaaacc
     4021 cagcatatac caccaaatag aatatcctga taacgttcaa tttaacctaa ctggtttttc
     4081 taatgaggac ctcttagaag ttcgattgaa gtattcttac ggtaattcaa aaataatatc
     4141 ttataaatat attgaccctc cttcaaaagg ggaaatccgg tcagaagtta ctttgaatac
     4201 cagtggacct aattacatcc ctgcgggaac gcaaattaca tatttttttg acttgacttc
     4261 agttaaaggc aaaaagtacc aatctcctca aatcaaattt cactatctag atccatcaat
     4321 taaatggaaa tccactgaac tgaatcattt gactttaatt tggcatgaca gaaacgaaag
     4381 tgatatcaac tctgtgattg aagatgtgaa taaatcgaat ttacgtttca ctaaactatt
//
LOCUS       DASCNS010000091         2102 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000763758, whole genome shotgun sequence.
ACCESSION   DASCNS010000091 DASCNS010000000
VERSION     DASCNS010000091.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000763758"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="QF838_03365"
     CDS             <1..440
                     /locus_tag="QF838_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HJO59762.1"
                     /translation="IDERYTLYAIILGCGTTGIKVAEWLMASGAEVTLIEEIGEKQRL
                     ADNLLGSVVVLGDGTKIATQNIAGVKRANLFISTLSSDEDNMLACQIAKHHFEVDRTI
                     AISDTPDNANLLRLLGIDIVIDVTELITEKMQSLVAPMLVEDL"
     gene            437..1273
                     /gene="pheA"
                     /locus_tag="QF838_03370"
     CDS             437..1273
                     /gene="pheA"
                     /locus_tag="QF838_03370"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HJO59763.1"
                     /translation="MNKTVRKIAYLGPPGTYSEQAAKQWNNVDELLPVESIPAVAKSV
                     EEGESYQGVVPIENSIEGGVTFTLDLLIHDSTLSICGEVIVPINHYLMAQKEIDLENI
                     TTVFSHPQSLGQCRQFLLNNIPQATLMASLSNAKAVEEMLDSTPNSAAISSERSANLY
                     GAVVLQKNVEDNPNNETRFVILSQDDHEITSNDKTSLCFEFDGDSPGILSESLTEFAA
                     RNINLVKIESRPNKRRLGRYVFLVDIDGHRRTVEVKQALDSLQKKVSMLKIFGSYPKG
                     DV"
     gene            complement(1289..1651)
                     /locus_tag="QF838_03375"
     CDS             complement(1289..1651)
                     /locus_tag="QF838_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HJO59764.1"
                     /translation="MAKDNGGGFSTGLILGAIIGTVAGILMAPKPGSETRSELLEKSD
                     YWREKAERFSDIIVERLGPTLETVRDNVPPTIENMKARIDPLIEQVNSRFVGPDTNVS
                     EEPHSETGINSDDSAKPS"
     gene            complement(1651..1923)
                     /locus_tag="QF838_03380"
     CDS             complement(1651..1923)
                     /locus_tag="QF838_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59765.1"
                     /translation="MDSNIYEIVGLIRDMAFIVCLPLICIFLILTIVKINRLLESLTR
                     NFQNVENITDNIAGKFSGSMGLFGGLIKLFTWAGGFSRKNHRKGDD"
BASE COUNT          609 a          422 c          444 g          627 t
ORIGIN      
        1 ggattgatga gaggtatacc ttgtacgcga taattttagg atgtgggacc accggtatta
       61 aagtagctga atggttgatg gcctctggtg ctgaagtaac actgatagag gaaattggtg
      121 agaaacagag acttgcggac aatttgctgg gctcggtggt ggttttgggt gatggtacta
      181 aaattgctac tcaaaatatc gcgggtgtaa aaagggctaa ccttttcata tccaccttaa
      241 gctctgacga agacaacatg ctagcatgcc aaatagccaa gcaccatttt gaagtagaca
      301 ggaccattgc tataagtgat actccagaca atgccaacct gcttaggctt ttggggatcg
      361 atatcgtaat cgacgtgaca gaacttataa ccgaaaaaat gcaaagcctc gtcgctccca
      421 tgcttgtgga ggatttgtga ataaaactgt tcgtaaaata gcgtatcttg gtcctcccgg
      481 aacttacagt gagcaggcag ctaagcagtg gaacaatgtt gatgaacttt tgccagtcga
      541 atcgattcca gctgttgcaa aatcagtgga agagggagaa tcttatcagg gagtcgtgcc
      601 tatagaaaac agtattgagg gtggagtcac tttcacactt gatttactca ttcacgattc
      661 aaccttatcg atttgtggtg aagtcatagt ccccataaat cattatttga tggctcagaa
      721 ggaaattgat cttgaaaata tcactactgt attttctcac ccgcaatcat tgggtcaatg
      781 taggcaattt cttctgaata atataccgca agctaccttg atggcttcct taagcaacgc
      841 taaagctgtt gaggagatgt tagattcgac accgaactca gctgctatat caagcgaaag
      901 atctgctaat ttgtatgggg cggtagttct gcaaaaaaat gtagaggaca accccaacaa
      961 tgaaaccagg tttgttattc tttcacagga tgaccacgaa ataacttcaa atgataaaac
     1021 ctctctttgc tttgaatttg atggagatag ccccggtata cttagcgagt ctttgacaga
     1081 attcgcagct agaaacatca atcttgtgaa gattgagtcg agaccgaata aaagacgttt
     1141 aggccgttat gtttttctgg tagatatcga tgggcacagg agaaccgttg aagttaaaca
     1201 ggcactggac tcgcttcaaa aaaaagtgtc catgctgaaa atttttggtt cctaccctaa
     1261 aggtgatgtg tagcgcttag atcctatttt atgagggttt tgctgaatcg tcactattaa
     1321 tacctgtctc tgaatgaggt tcctcagata catttgtgtc tggacctaca aatcttgaat
     1381 tcacttgctc tattaagggg tcaatccggg ctttcatatt ttctattgtt ggaggtacgt
     1441 tatctcgaac tgtttcaagt gtcggaccta gcctttctac tattatgtcg gaaaatcttt
     1501 cagctttttc tcgccaataa tcacttttct ctaaaagttc ggaccgggtt tcagacccag
     1561 gtttaggagc cattagtatt ccggcaacgg ttcctataat tgcccccaag ataagtccgg
     1621 tggaaaagcc accaccattg tctttagcca ttagtcgtct ccttttcgat ggttttttct
     1681 ggaaaatcct ccggcccagg taaacaattt tataaggccg ccaaaaagtc ccatgctccc
     1741 cgagaattta cctgcgatgt tatcagtaat gttttccaca ttttgaaaat tcctagtaag
     1801 ggactccaac aggcgattga tttttacaat agtcaaaatt aagaaaatgc aaatcaatgg
     1861 gaggcaaact atgaatgcca tgtcccgtat taagccaact atttcataaa tatttgagtc
     1921 cataacagtt ctttaatcct agcataatag aaaaacactc gacgaaatga atcatcgagt
     1981 gcatcctaat ttttgatttg ctgctgcctt atggcttaat aagcattctc acagaacctt
     2041 ctgcatcttt tctctgagta acaatgtcat ggctgttttt ttctgcccag actattaagt
     2101 ca
//
LOCUS       DASCNS010000092        15226 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000776238, whole genome shotgun sequence.
ACCESSION   DASCNS010000092 DASCNS010000000
VERSION     DASCNS010000092.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 15226)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15226)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15226
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000776238"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="QF838_03385"
     CDS             <1..496
                     /locus_tag="QF838_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HJO59766.1"
                     /translation="SSEPPDFLQSDKDLENANNVSRRFISYRPRSEGELRIRLMKIFS
                     HEIVLDSVAQMYREGLLNDQRFAQMWVDSRQQSRPKSKKSIKQELLSKGISEHFAEKA
                     ISQLSDIENATLCANKKARSLNGLGKEDFYKKLEGYLQRRGFNFSISRTVISEAWNNN
                     QSSF"
     gene            571..1890
                     /locus_tag="QF838_03390"
     CDS             571..1890
                     /locus_tag="QF838_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013741.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HJO59767.1"
                     /translation="MSTDTAVSIVLIGVFLLLSAFFSSSEAAFLSLQRTRLNYLVTNN
                     IPGAKRVHDMVTNTERLLSTILLGNNLVNVAFTAVVTALAVKWLKEGPVAVAAATIVG
                     TGLLLIFGEIIPKSLAVKKSERVSFLYARPLKLIELSLYPLILFLQWLSRTTQSIFGK
                     EQEEVVTEGEILSMIDLGEAEGTVEPNEAEMLENVFRFGDTQVREIMTPRTEIIVMDR
                     GTTLNDFLNIYSEHSHTRFPIYKDTNENIVGIISAKDVLRILSTKGINGNDSVTDVIR
                     DAHFVPETKWASELFEELRHAGNQMAICLDEYGGLAGLVTLKRLTELIVGKVGEEGES
                     PEIEYENIRPNVFQIEGGMDIADANSEMDLELPEGEYETIAGFVLDQIGEIPKINDEF
                     EYEDMTFRILEMDRFRVESLLITRNPNPMKTPKDSKSDKILDADNTG"
     gene            1871..2479
                     /locus_tag="QF838_03395"
     CDS             1871..2479
                     /locus_tag="QF838_03395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJO59768.1"
                     /translation="MQIILVRHGETIANKEQLVLGTSDVPLTELGRDQAKAAAKKISL
                     MDPSPTILFSSPYQRAKETARYISNYTNLNPIFIDGLKEMNSGEMEGIKASDMNDKYP
                     DYMKSWRRDHSTARPPGGETLGEVHTRAWKSILNIFDEYNESLVVVVAHLFPIQGILC
                     NVLGIKSNNFEKLVIDLGSLSFIEIENDSSRLLRMNDICPID"
     gene            2510..3937
                     /gene="tilS"
                     /locus_tag="QF838_03400"
     CDS             2510..3937
                     /gene="tilS"
                     /locus_tag="QF838_03400"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HJO59769.1"
                     /translation="MKPESLRETIINSVHSNNLSGRPLSLAVSGGPDSMAMAHVLITS
                     NPKLHSEIEILHFNHQIRGKEAKSDSDFVKNFFKLLDVPTFVSQGNVLALSKKNKMSV
                     EAAARTARFEFFATHISQSKRNSVLTLGHNFDDQAETILMHAIRGSGLNGLQGMAKLS
                     TQTIQGLALKIFRPMLSVSRESILKYCEDYDIPWKADSTNFSTEYTRNSIRLELIPSL
                     KKYNPEISKSLVKLGNLARRDHDYIQSKIEEIWPSIATTSNGVIVLNINRLQRLDASL
                     TWRILKKAATDMKGDEHNLTYNHVELMLDITKGISGRSVNLPGKVIATKSYDTLLISK
                     SNDDFLPAPVLNRPTGIATPGTTVTDRWKISAFLKPCNFSSENKNVKRVKSTFLNKDL
                     MNQSLWVRSRNPGDVFQPSGMKNTKKLQDFMVDSKIPRIDRDSVPLIVSNMGIAAVVG
                     WRVAEWAMPSKSEHSLYIEFDPEKY"
     gene            complement(4006..5037)
                     /locus_tag="QF838_03405"
     CDS             complement(4006..5037)
                     /locus_tag="QF838_03405"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HJO59770.1"
                     /translation="MTTPQFDIRPSLIKGDPADAYLHWTKEVMRLESLNPKVLMDFST
                     PNPGVLCGVEEAKALLLNVLPKVEQDRQASNGGDIVQVWAMEEGEKFEAGEPVLRVIA
                     KYASFGLYETSILGMLSSSSGWATASNECVEAAGETTVIAYGARHIHPNVAHILDYAS
                     VVGGCSSVSTILGSKHAGRNPLGNMPHSLPLLFGDTVAAAQAFDKHIGMETQRIVVVD
                     TFKDEAEESLNVAEALRDRLRGIRLDTPAERGGVTPMLVKEIRNRLDHMNFKHVEIYV
                     SGGFTPEKIQEFQELKAPVNGYLVGSYISSAVPNEFRADILEIEDKPVAKRGRVPGRL
                     DGNRLDRIL"
     gene            complement(5168..5926)
                     /locus_tag="QF838_03410"
     CDS             complement(5168..5926)
                     /locus_tag="QF838_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HJO59771.1"
                     /translation="MSGFSNSLDIVIPVLNEEKALENSIHTLVSFCQNKIGHYDWVIT
                     VADNGSTDQTLQIAEMLSEQYSRVRFIRLEERGRGRALKMAWSQSEAAILAYMDVDLS
                     TDLNCLPQFLEPLNSLKFQIVIGSRLISGSKVVGRSFKREFISRCYSILFRTMFFVSF
                     KDAQCGFKAISRQVAQEVVPLVKNNGWFFDTELLILAEKNGYPVLEIPVKWVDDPDSR
                     VDVLKTAVEDIKGLLRLRFQDLDKSRKLLKFNKS"
     gene            complement(5951..7471)
                     /gene="purH"
                     /locus_tag="QF838_03415"
     CDS             complement(5951..7471)
                     /gene="purH"
                     /locus_tag="QF838_03415"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HJO59772.1"
                     /translation="MKALLSVYNKREIELMGIALSEAGYDIVSTGGTYNSLKKAGVPV
                     KQISDLTGSPEILNGRVKTLHPKVHGGILARRDIPEHLEEIESHGISEIDVVVANLYP
                     FVDTVKKDDVSLSEALENIDIGGPTMIRSAAKNFPDVLVIVDPSDYGWVAEKISCGQS
                     FTKEERKKLAQKAFQHVAVYDTARSTWLSEAPLESTELSIGLNKVTDLRYGENPHQDA
                     VLYRDTLGEGGIVQAEQLHGLPMSYTNYLDADAAWTTVTSFSQTACVVVKHTNPCGIS
                     VDDDQASAYEGAFQGDSISAYGGIVGFNTVVTKKTAESMRGVLFDIIVAPDYEVDALD
                     ILRKRKRTRVLKARNDSGPFSNLEALTITGGMLIQTSDRIQEDPATWKIVTDRHPTET
                     ELRDLEFAWSCLRHIKSNTIVLAKDTTLVGMGAGQPNRLVSVHLALRISGDRSIGSTL
                     ASDAFMPFADNVELAARGGITAIIQPGGSIRDEEVIEAANHHSISMMFTGIRHFNH"
     gene            complement(7494..8528)
                     /gene="purM"
                     /locus_tag="QF838_03420"
     CDS             complement(7494..8528)
                     /gene="purM"
                     /locus_tag="QF838_03420"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HJO59773.1"
                     /translation="MKDDKITYESAGVNLLASEKIKDRIKNLAEKTYTPNVLGGVGGF
                     GAMYKISGYRDPVLVSSTDPVGTKLMVAGMVGDYSYIGEDLVNACVNDLIVVGAEPLF
                     FLDYIATSTMVPEVVENIVTGISEACKNVGCALIGGETSEMPGVFKEGKFDISGFVVG
                     AVEADEMLNPLDTVREGDLLIGLPSNGLHTNGYSLVRHVFNLENDLGILKQRLNKSGE
                     TLGDALLKPHPSYYHDLKSIFSDSKGIAHITGGGLYENMPRILPKDIEAQFDASQWDV
                     PDVFEFIRKTGLVDSNEMYRVFNMGLGMVIVCVPEKASRILKNVPSAKIVGELKQRSS
                     DNRVTIENKR"
     gene            complement(8552..9181)
                     /gene="purN"
                     /locus_tag="QF838_03425"
     CDS             complement(8552..9181)
                     /gene="purN"
                     /locus_tag="QF838_03425"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HJO59774.1"
                     /translation="MNELGAKSISLGILASHGGSNLQAIMDACEDGLLKANVAVVVSN
                     NSRSLALQRARKSGVPAYHLSSNTHSDDGDLDLAIKDILKKYNVDLIILAGYMKKIGP
                     SVLRTFRNRVINSHPALLPNYGGKGMYGDRVHKAVVKAKSAETGISIHLVDEDYDHGA
                     IINQKIIAVDSSEGVEFVKKKVQKHEHTFWITTLNKIQKGEIDLDNVGH"
     gene            complement(9198..10583)
                     /gene="purF"
                     /locus_tag="QF838_03430"
     CDS             complement(9198..10583)
                     /gene="purF"
                     /locus_tag="QF838_03430"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HJO59775.1"
                     /translation="MKEACGIVGVFAREEDVARSVFFGLYALQHRGQESAGISASDGH
                     RIRLHTEMGLVSQAFQENDLSHLSGHIAIGHTRYSTTGSSNINNAQPILSKGTSTEIS
                     LAHNGNVINAMDLREELLDWGIQFQSSGDSEIIAHLIANAPVENWPDRIAYIMRRLQG
                     AYSLVVMTNQEVLGVRDPLGVRPLCIGKLNSGWVLASESCALDHIGAEFVREVEPGEA
                     VLINDNGITSIYKKPKEGVFGRCVFEHIYFSRPDSLLDGQLVYSARMAMGAQLAKEFP
                     VEADLVIGVPDSATASAVGYSQESGIPFTEGLVKNRYVGRTFILPDQRLRELGVRRKL
                     NLLPQILEGKRVIVVDDSIVRGTTTPHVVNLLRKGGAKEIHLRICAPPIVSPCHFGVD
                     MATKAELMASSNSVNEIREIIGADSLGFLSEEGLLKSVSGKRNEYCMGCFTGKYPIPV
                     QLEMDKLVLET"
     gene            complement(10845..11933)
                     /locus_tag="QF838_03435"
     CDS             complement(10845..11933)
                     /locus_tag="QF838_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014381.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HJO59776.1"
                     /translation="MLGRKVTILGGGNTAFSVAARLAHMGNSICLLEHPDFMESISEI
                     MVTKTINLEGVLETGPAIIDKITLDPLEALEFSDLLLLIVPAYAHKPFAELCGPYLTE
                     DHTVVIMPGTIGSLEWKVLASEFGAFNMTVAEVDTAPYVCRKTSNGSAVIWGEVASLG
                     MGVIPSSDTSSVKDMLQPLFPGIRAYSDSLECGLSAMNPVVHPAGVLMNAGRVEYSRG
                     EFYFYEEGVSGSVAKAIEAVDEERRNIGKALGYDLVPVAEAFHKAGFGPRGQIWEVIN
                     GSYMLTRLKAPGSLDSRWLSEDIPFGISAWSKIGKQYGVSSPVMDSFVQIGNVVMGAG
                     GNEGRGPKDFGIENLSISELQKYLQTGT"
     gene            complement(11972..12199)
                     /locus_tag="QF838_03440"
     CDS             complement(11972..12199)
                     /locus_tag="QF838_03440"
                     /inference="COORDINATES: protein motif:HMM:NF021381.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJO59777.1"
                     /translation="MSNEPENIKHYECPKCSNTSYEAGEIRTTGSGLSRFMNLQNQKF
                     AHITCDACGYTEFFKRRSRGAGNILDLFLGG"
     gene            complement(12276..13811)
                     /locus_tag="QF838_03445"
     CDS             complement(12276..13811)
                     /locus_tag="QF838_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59778.1"
                     /translation="MNARTKKFIPAILLVVCVFLFGSTANAHGFGERYDLPIPLTYFL
                     IGAALTVAFSFVVIGWFIRSSGSDLKYPRINVYRYSAMAFFCKAISRCLGLISVFILF
                     ISIHSGLTGTSNAIENFAPVFVWIIWWVGVGYVVCIVGNIWLVMNPWLVIFNYWERIF
                     GKHMGLVGWPAKFDAWPSLVLFLLFAWIENVHPASSQPYSLGILILIYSLLTWGGMIL
                     FGKHVWLTHGDPFHVLFNLFARFSATELRINGTKNWCTRCASGCKENLNLPDCVDCYE
                     CWENTDSENKEFCLRPWSVGLSRGERVTPAIMLFHVTALATVSFDGFSETPAWIQIQT
                     VLWPAIDPLPGSAAATIETIGIILGPLIFAAIYLYVCGLISRMSHGQMTSVDTRKSFV
                     FSLVPIALAYNLAHYLSFLLITGQELIPLLSDPYGFGWNLFGTSDFKVNIAIIDARVA
                     WIFSVVALVIGHIISVLTAHVIALRKTTSHSIAVRSQYPMLLLMVFYTAVSLWIVAQP
                     IVE"
     gene            complement(13897..14658)
                     /locus_tag="QF838_03450"
     CDS             complement(13897..14658)
                     /locus_tag="QF838_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59779.1"
                     /translation="MNHKNLTMQVFWIAIFFALAMGCGSSDENSFSVAVDENTSVFDI
                     GISSGELVGDEGTLRIKKNENVRLNFSSDTEITVHLHGYDIEKTVFSGNDQVMEFKAN
                     ATGRFAITSHKNETGHQDHGSHSDHSGAEIDADTHAALFESETLTSGEMFSYQVPMNI
                     ENTTIPYHDHMNHDAGGSIEVSSDHGESGNVLVMVGSAGHYFHPDNVIVKPGALIEWK
                     IESDKKVRLTSGLPPSNQANHESEEETLITIEVYP"
     gene            complement(14658..>15226)
                     /locus_tag="QF838_03455"
     CDS             complement(14658..>15226)
                     /locus_tag="QF838_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59780.1"
                     /translation="SKVEVKMLWFRAAIFVSLLFLGLYNQSGTLLEANGSIRSYEKKV
                     IGEYVIGLGTTPPSPSIGVTHFAAFVENEQTGTRYVDVEIEFVSSSSTIDLPEVGPEL
                     MKNSIMDPMYYEIDTSFNKEGVWLVMLTVRREGNEVSVVYEVEVKKPNPIVPILTVGT
                     LLFFLAILSLSLRSWIKEYRKKFKIRKA"
BASE COUNT         4626 a         3481 c         2733 g         4386 t
ORIGIN      
        1 ttcatcagaa ccacctgact ttttgcaaag tgacaaagac ctcgagaatg ccaacaatgt
       61 atccaggcga ttcatctcat atcgacccag aagtgagggc gagctacgaa tacgactaat
      121 gaaaatattt tcccatgaaa tagttttaga ttcagttgct caaatgtata gagaaggatt
      181 acttaatgat caaaggttcg ctcaaatgtg ggttgatagt aggcaacaat ctcgaccaaa
      241 aagtaaaaag tctattaaac aagagttgct ttcaaaagga atttcagaac attttgcaga
      301 aaaagccatt tcacagctaa gcgatattga gaacgctacc ttatgcgcta ataaaaaagc
      361 tagatcacta aatgggcttg gaaaagagga tttttataaa aaactggaag gctatttaca
      421 gagaagaggg tttaattttt ctatctcccg cacggtcata tcagaagcct ggaataacaa
      481 tcaatcttct ttttaaaaca ccgccaacgc aatcaatcta taattatctc cataatctgt
      541 ccggtgttac gtagatattg gaggtagttt ttgtcgaccg acacagctgt aagcatagta
      601 ctaattgggg tatttcttct tctgtctgcc ttcttttcaa gttcagaagc tgcttttcta
      661 tctcttcaaa gaacacgtct aaattatttg gtgactaaca acatacccgg ggcaaaacgt
      721 gttcacgata tggtgactaa cacagaaaga ttactttcaa ccatattatt aggcaataac
      781 ctagtaaatg ttgcctttac agcagtagtc acggccctag ctgtcaaatg gttaaaggaa
      841 ggccctgttg ctgtcgctgc ggctacgata gtaggtacag gattgctgtt aatatttgga
      901 gaaataatcc ccaaaagtct tgctgtaaaa aaatcggagc gagtgtcgtt tttatacgcg
      961 aggcctctga aattaataga actttcccta taccccttga ttctattcct tcagtggtta
     1021 tcaaggacca cccaatctat ttttggaaag gaacaagaag aagtagtgac agaaggcgag
     1081 attttatcaa tgatagatct tggtgaggct gaaggcactg tagagccaaa tgaagccgaa
     1141 atgctagaaa atgttttccg ttttggagac acacaggtca gggagataat gaccccacga
     1201 accgaaatta ttgttatgga ccgagggacc acccttaatg acttcctaaa tatttactct
     1261 gaacactctc acacacgatt cccaatttat aaggatacaa acgaaaatat tgttgggatt
     1321 atttctgcca aggatgtgtt aagaatttta tcaaccaaag gaataaatgg aaatgattct
     1381 gtaactgatg ttattcgaga tgctcacttt gttcccgaaa caaaatgggc atccgaactt
     1441 tttgaagaat tacgacacgc tggtaaccaa atggcgatct gccttgatga gtatggtggc
     1501 cttgccggtc ttgtgacgct aaaaagacta acagagctga tagtcggtaa ggttggtgaa
     1561 gagggtgaat cgccagaaat tgaatacgaa aacatcaggc ccaacgtttt ccaaattgaa
     1621 ggtggcatgg atatagcgga cgctaacagc gagatggatt tagaactacc agaaggcgaa
     1681 tacgagacaa tagccggttt tgtacttgat cagataggtg aaatacctaa gataaatgat
     1741 gagtttgaat atgaagatat gacctttcgc atattagaaa tggaccgatt tcgggttgag
     1801 tctctactaa tcacaagaaa tccaaaccct atgaaaacac ccaaagattc aaaatcggac
     1861 aaaatacttg atgcagataa tactggttag acacggcgaa acaatagcga ataaagaaca
     1921 actagtcctc ggaacaagtg acgtaccttt aacggaatta ggaagggatc aagccaaagc
     1981 agctgcaaaa aaaatatctt taatggaccc ttctcccact atcctattct caagtccata
     2041 ccaaagagca aaggaaactg ccagatatat ttctaactac actaacctaa atcccatctt
     2101 tattgatggc ttgaaagaga tgaattccgg tgaaatggag ggcatcaaag cctcggacat
     2161 gaatgataaa tatcctgact atatgaaaag ttggagacgt gaccattcta ctgccagacc
     2221 tccaggtggt gaaactcttg gagaggtgca tacaagagct tggaaatcca ttctaaatat
     2281 ctttgatgaa tataatgaat ccttggttgt tgttgtggct caccttttcc cgatacaggg
     2341 catactctgc aatgtattag gaattaaatc caacaacttt gaaaaactag ttattgatct
     2401 aggatcattg tcattcattg agattgaaaa tgactcatct cgcctactta gaatgaacga
     2461 tatatgcccc attgactgac tctaaaaacc cctttcaaag gtgttgtaca tgaaaccgga
     2521 atcacttcgg gaaaccatta taaattcagt acatagcaac aatctttcag gtcgtcctct
     2581 ttcacttgcg gtttctggtg ggcctgattc catggcaatg gcccatgttt taattacaag
     2641 taaccctaag ctgcattctg agattgagat tcttcatttc aatcatcaga tcagaggcaa
     2701 ggaagcaaaa tcagattccg acttcgtgaa gaatttcttt aaattgttag atgtcccaac
     2761 ttttgtatct caaggcaatg ttctagcact ctctaagaaa aataagatgt cagtggaagc
     2821 ggcagctcgc accgcgagat ttgaattctt tgcaacccac atctctcaaa gtaaaaggaa
     2881 ttcggttctc actctagggc ataattttga tgaccaagcg gaaactatac taatgcatgc
     2941 tatccgaggt tctggtctaa atggacttca aggtatggcc aagttatcaa ctcaaacaat
     3001 tcaaggattg gctttaaaaa tatttcgacc gatgctttcc gtgagccgag aatccatact
     3061 caaatattgt gaggattacg atattccgtg gaaagcagac agtacgaatt tttcgacaga
     3121 gtatacccgt aattcaatcc gattagagtt gattccgtct ttgaaaaaat ataaccctga
     3181 aatatccaaa tctcttgtta aattaggaaa tttagctagg agggaccatg attatataca
     3241 atcaaaaata gaagagattt ggccttccat tgccacaaca tcaaacgggg ttattgtact
     3301 caatataaat cgacttcaac ggttagatgc ttctttgaca tggagaattt taaaaaaggc
     3361 cgccacagac atgaaaggcg atgagcataa cctgacatat aatcacgtcg aattgatgtt
     3421 agatataact aaagggattt caggacgctc agttaatctt ccgggaaagg ttattgcaac
     3481 taaaagttat gacactcttt taatatccaa gtctaacgac gattttttac ccgcacctgt
     3541 tttgaatcgc cccacaggga tagcaactcc aggaactact gtcaccgatc gttggaaaat
     3601 atcggcattt ttgaaaccct gtaatttttc ttctgagaat aagaatgtaa aaagggtgaa
     3661 atcaacgttt ctaaataaag atttgatgaa tcaatcatta tgggtgcgtt ctcgaaatcc
     3721 aggcgatgtg tttcaaccat ctggcatgaa aaacactaaa aagttacaag atttcatggt
     3781 ggattcaaaa ataccacgaa tagatagaga ttctgttcca ctaatagttt caaatatggg
     3841 aatcgctgcc gtggtaggtt ggagggtagc agagtgggcg atgccatcaa aaagtgaaca
     3901 ttctctttat attgaatttg atcctgaaaa atattgaaat tggtttttta acgccatcca
     3961 attccaaatt atatcctcac aggaacgagg tctattagct caaaactaaa gaatcctatc
     4021 taatcgattt ccgtcaagac gacctggaac acggcctctt ttagcaactg gtttatcttc
     4081 tatttcaaga atatccgctc gaaattcatt tgggacagca gaagaaatgt aactaccaac
     4141 taaatatcca ttaacaggtg ccttcaactc ttgaaactct tggatttttt caggagtgaa
     4201 tccaccactt acgtatattt ccacatgctt gaaattcata tggtcaagtc gatttcgaat
     4261 ttccttaacc aacatgggag taacaccacc cctttcagct ggtgtgtcca accttatccc
     4321 cctaagccga tccctgagag cttccgcaac gtttagagat tcttctgctt catccttaaa
     4381 agtatcaacc accactattc gttgagtttc cataccaata tgcttatcaa atgcctgggc
     4441 agcagcaact gtatccccaa acaataaagg taagctgtga ggcatgttcc caagtgggtt
     4501 ccttcctgcg tgtttggacc ccaaaatagt agaaacggaa gaacaaccac ctacaactga
     4561 agcgtagtcg agtatgtgcg ccacattggg atgtatatgc cgagcgccat aagctattac
     4621 cgtggtttct ccagccgcct caacacattc attggaggca gtagcccatc cactagacga
     4681 tgaaagcata cccaatatgc tagtttcgta gagaccgaat gaagcatatt tcgctataac
     4741 tcgcaaaacg ggttctccag cttcaaattt ttcaccttct tccatcgccc aaacttgtac
     4801 gatatcgcca ccgttacttg cctgtcgatc ctgttcaacc ttgggtagta catttaaaag
     4861 cagagcttta gcttcttcaa ctccacataa aacaccaggg ttaggggtcg aaaaatccat
     4921 taacacctta gggttcaaag attccaaacg cataacttct ttggtccaat gtaagtacgc
     4981 gtcagccgga tcacctttga ttagtgatgg tcttatatca aattgaggtg ttgtcattca
     5041 gcaggcctac agcacagtaa gaaagctttt cttctgttcc taattgtata atttacctgt
     5101 cgtcactagt gaaggaattt gtgggtttcc aattgtactc tcgccggtct gccatagcaa
     5161 tctagtctca tgatttattg aatttaagca attttcgaga tttatctaaa tcttgaaatc
     5221 taagtcttaa tagacctttt atatcttcaa ccgcagtttt tagtacatcc actctagaat
     5281 ctggatcatc cacccacttc actggaattt ctaaaaccgg ataaccattc ttctcagcca
     5341 atatcaaaag ctccgtatca aaaaaccaac cattattttt gacgagcgga acaacttctt
     5401 gggccacttg tctagatata gctttaaatc cacattgagc gtccttaaag gagacaaaaa
     5461 acatcgttct aaagagaata ctataacaac gtgagataaa ttctctttta aaagaccttc
     5521 ccactacttt ggaccccgaa attaatctag atccgataac gatctgaaat tttaaactat
     5581 tgagtggttc taagaattga ggtaaacaat ttaaatctgt ggaaagatct acatccatgt
     5641 aagccaatat cgctgcttcg ctttgagacc aagccatttt aagtgccctc cctctacctc
     5701 tctcctccaa ccgtataaac cgaactctgg aatattgttc tgataacatt tcagcgatct
     5761 gcaaggtctg atcggtggat ccgttgtcag ctactgtaat aacccaatca taatgtccga
     5821 ttttattttg gcaaaatgaa acaagggtat gaatgctgtt ctctaatgcc ttttcctcgt
     5881 taagtacagg tatcacaata tctaaagaat ttgaaaatcc gctcaaaata ttcccctaga
     5941 gcatcattag tcagtgatta aaatgcctta tccccgtaaa catcatagaa atagagtgat
     6001 gatttgccgc ctctattact tcttcatctc ttatagaccc gccaggttga ataatagcgg
     6061 tgattccacc tctggcagcc aattccacat tatcggcaaa tggcataaag gcatcagaag
     6121 ctagagttga accaatggac ctatctcctg aaattctcaa tgcgagatgc acactcacaa
     6181 gcctatttgg ttgtcctgct cccatcccca ctaatgtggt gtctttagct aaaactattg
     6241 tgttggattt aatatggcgc aagcagctcc atgcaaattc aaggtctctt aattcagttt
     6301 cagttggatg cctatcagta acaatcttcc atgtggcagg gtcctcctga attcgatcag
     6361 atgtttgaat taacatacca ccagttattg tcaacgcctc taggtttgaa aacggtccag
     6421 agtcgttgcg agccttaaga acccgggttc ttttcctctt cctcaaaata tccaaggcat
     6481 ccacttcata gtcaggcgca acaattatgt cgaacaaaac tccacgcatt gactcggctg
     6541 ttttttttgt taccacggta ttaaacccaa ctattccccc ataagcagaa atagaatcac
     6601 cttgaaaggc gccttcatat gcagaggctt gatcatcatc cactgaaatg ccacaggggt
     6661 tcgtatgctt tacaacgaca caagctgtct ggctaaaact tgttactgtt gtccatgctg
     6721 catctgcatc caaataattc gtgtaagaca tcggtaatcc atggagttgt tcagcctgta
     6781 cgattccccc ttcacctaat gtatcccgat ataagacagc atcttggtga gggttctccc
     6841 catatcttag atccgttacc ttattaagcc caatggataa ctccgtagat tccagaggag
     6901 cttcgctcag ccaagttgat cttgcagtat catacacagc cacatgttgg aaagcttttt
     6961 gagctaactt ttttcgttcc tctttggtga aactttggcc acaagaaatc ttctcagcga
     7021 cccaaccata atcacttgga tctacgataa ccaatacatc gggaaaattt ttagcggccg
     7081 accgaatcat tgtcggtcca cctatatcga tattttccaa tgcctcagac aatgaaacat
     7141 cgtctttttt cactgtatcg acaaacggat ataaattcgc taccaccacg tctatttcag
     7201 atatgccatg tgattctatt tcttctaaat gctcaggaat gtcacgcctg gccaaaattc
     7261 caccatggac tttcgggtgc aacgtcttca ctcttccatt caatatctca ggggaacccg
     7321 tgagatccga tatctgcttc actggaacgc cagctttctt cagagagttg taagtgcctc
     7381 ctgtactcac gatatcgtat ccagcttcac taagagcaat tcccataagc tctatttctc
     7441 ttttgttgta gacacttaat aaagctttca taaacccctt ctggttttga taatcacctt
     7501 ttgttttcta ttgtgactct attatcagaa cttctttgct ttaattcccc tactattttt
     7561 gcactaggaa cattctttaa aatcctagaa gccttttcag gaacacaaac aatgaccatt
     7621 cctaaaccca tattgaaaac tctatacatc tcattagaat caaccaaacc agtttttctg
     7681 ataaattcaa aaacatcagg gacatcccac tgagaagcat caaattgagc ttctatatct
     7741 tttggcaaaa ttcttggcat attttcatac aatccacccc cagtgatatg tgctatgcct
     7801 tttgaatcgg agaaaattga ttttaaatca tgatagtaag aagggtgagg cttcaaaagt
     7861 gcgtctccta gggtttctcc tgatttattt aatcgttgct tgaggattcc caaatcgttt
     7921 tccaagttaa aaacgtgccg tactaaagag tatccgtttg tatgcaaacc atttgatggc
     7981 aggccaatga gtaaatcacc ttctctaacg gtatccaatg gatttaacat ttcatccgcc
     8041 tctacggcgc caacaacaaa accagaaata tcaaatttac cctccttaaa aacccctggc
     8101 atttcagaag tttctccccc aataagggca cagcccacat ttttgcacgc ttcagagata
     8161 ccagtaacaa tattttctac tacctctgga accatggtgg atgtagcaat atagtcgaga
     8221 aaaaacaatg gttctgcacc aactacaatg agatcattca cacaagcatt aactagatct
     8281 tccccaatat agctataatc accaaccatc cctgcaacca tcaatttcgt acctaccgga
     8341 tcagtgctgc tgaccaaaac tgggtcacgg taaccggaaa ttttatacat cgctccaaag
     8401 ccaccgaccc cacccaatac attaggggta tatgtttttt cggctaaatt ttttatccta
     8461 tctttgattt tttctgatgc cagtaggtta accccggctg attcgtaggt aattttgtcg
     8521 tctttcatga tcttatgtag ttaatactgg tttaatggcc aacattgtcc aaatcaattt
     8581 ctcccttctg aattttattg agtgtggtta tccagaatgt atgctcatgt ttttgaacct
     8641 tttttttcac aaactcaacc ccttcagaag aatcaacagc tattattttt tgattaatga
     8701 tcgcaccatg gtcgtaatcc tcgtctacca agtgtatcga aatcccagtc tccgctgact
     8761 tagctttaac tacagcctta tgcacccgat ctccatacat tccttttcca ccgtagttgg
     8821 gcagtaaagc tgggtggcta ttaataactc tattgcggaa tgttctcaga acagacggtc
     8881 ctattttctt catgtatcca gccaaaatta ttaaatctac attatacttt tttaaaatgt
     8941 ctttaatggc aagatctaag tctccatcat cagaatgggt gttggaactt aaatggtatg
     9001 ccggtacgcc tgactttctg gctctttgaa gagccagtga cctcgaatta ttactcacta
     9061 ccactgccac atttgccttt aataacccat cttcacaagc atccattatg gcttgaagat
     9121 ttgatcctcc gtgcgaggct aaaattccca gggagatgct tttggcaccc agctcattca
     9181 atagttaata cctattttta cgtttctaaa actagtttat ccatttcgag ttgaactggt
     9241 atcggatatt tgccagtaaa acagcccatg cagtattcat ttcttttccc agacacagac
     9301 ttcaacaaac cttcttcgct taaaaaccct aatgaatcag ccccaattat ttcccttatt
     9361 tcgttgactg agttactact agccatcaac tctgctttag tagccatatc cacaccaaaa
     9421 tggcagggcg aaactattgg cggcgcacag attcgtaaat gaatctcttt agcaccccct
     9481 ttcctaagta gattaactac atgtggggta gtagtgcctc taactatact gtcatccacc
     9541 acaatcactc ttttcccttc taatatctgt ggaagtaaat tcaattttct tctaactccc
     9601 agttctctaa gtctctggtc aggcaaaata aaagttcttc ctacgtatcg atttttcacc
     9661 agaccttctg taaaaggaat acccgattct tgtgaatatc caacggccga agccgtggca
     9721 gaatctggga ctcctattac taaatctgct tcaaccggaa actcttttgc gagttgggct
     9781 cccattgcca ttctcgcact ataaaccaac tgtccatcca gtagagaatc tggcctggaa
     9841 aaataaatgt gctcaaagac acatcttcca aaaacccctt ccttcggttt tttataaata
     9901 gatgtgattc cattatcatt gatcaaaacc gcctctcccg gttccacctc tctaacgaat
     9961 tcagcaccga tatgatcaag ggcacatgat tcggacgcca gaacccaccc ggaatttaat
    10021 ttacctatac aaagaggcct aacacctaga gggtccctaa cccctaacac ttcttggttg
    10081 gtcataacaa cgagggaata agccccttgt aatctgcgca taatgtatgc aatccggtca
    10141 ggccaatttt cgacaggtgc atttgcaatt aaatgagcaa taatttcaga gtctccagaa
    10201 gattggaatt gaatacccca atctaataac tcttccctaa gatccatggc attgatcaca
    10261 ttcccgttat gggcaagaga aatttcagtg ctagttccct tggatagaat cggttgggcg
    10321 ttattgatgt tgcttgaccc ggtagttgaa taacgggtat ggcctattgc gatatgaccc
    10381 gacagatggg ataggtcatt ttcttgaaag gcttgtgata caagccccat ttctgtgtgc
    10441 aatctaattc tgtgtccgtc actcgccgat attcctgcac tctcttggcc cctatgctgt
    10501 aaggcatata atccaaaaaa aactgaacgc gcgacatctt cctctctggc gaatacccca
    10561 actatcccac acgcttcttt catggtgtcg ccacctgtcg aaggaaattc cttcgaaatc
    10621 aagggattaa cctcagtatg gaagcgggac cggttaccct gttcgattac ctttactccc
    10681 taggaatgac ggtagtaagc acccagactc atctcaatcc aacccacgac tacaacgacg
    10741 accgaattat actgctcagt ctaattcatg gtcaaaccaa ttattcgatg gagttaactg
    10801 ttctacccac agataaaaat tgttagtggt aaagatattt attactacgt gccggtctga
    10861 agatattttt gcaattctga gatcgataaa ttttctattc caaaatcctt tgggcctcta
    10921 ccttcgtttc cgcctgctcc catcactaca tttccaattt gaacgaagga atccataact
    10981 ggagagctaa ctccatactg cttaccaatt ttactccaag ccgatatacc aaaaggaatg
    11041 tcttctgaaa gccacctgct gtccaatgaa cctggcgctt ttagtctagt aagcatataa
    11101 ctgccgttga taacctccca aatctgaccc ctaggcccaa atcctgcttt gtgaaatgcc
    11161 tccgcgaccg ggacgaggtc atagcctaag gctttcccaa tgttccttct ctcttcatca
    11221 acagcctcta ttgcctttgc cactgatcct gaaactcctt cttcataaaa ataaaactca
    11281 cctctagaat attccaccct accagcattc atcagaaccc ctgcaggatg aacaactggg
    11341 ttcatagctg atagaccgca ttctagggaa tcagaatatg ctctaatccc tggaaaaagt
    11401 ggctgtaaca tgtcttttac agaggatgtg tctgaggatg gaataacccc cattcccagg
    11461 gaggccactt ctccccaaat aactgcacta ccattagatg tttttctgca aacatacggg
    11521 gctgtgtcga cttctgctac agtcatattg aaagccccga attccgacgc caatactttc
    11581 cactctaacg acccaatggt gcctggcata ataactacag tatgatcttc agtgaggtac
    11641 ggaccacaca attcagcaaa cggtttgtga gcgtacgcag gtactatcag taacagtagg
    11701 tcagaaaatt ccaaagcttc aaggggatcc agtgtgattt tatcaattat agctggtcct
    11761 gtttctaaaa ccccttccaa atttatagtc ttggtcacca ttatttccga aattgattcc
    11821 ataaaatctg gatgctccaa taagcatata gaattaccca tatgcgctag tcgagccgca
    11881 acggaaaatg cggtgttccc gccccctaat attgtgactt ttctccctag catagtattt
    11941 ctcctcgggc cagcaacttt tgaaaaattc cctaccctcc tagaaataga tccaatatgt
    12001 ttcctgctcc tcggctgcgc ctcttaaaaa attcagtata cccacaggcg tcacaggtta
    12061 tatgagcaaa tttttgattt tgaagattca taaaccttga taaccccgaa cctgtagtcc
    12121 taatttcacc tgcctcatat gaagtatttg aacattttgg gcactcataa tgtttaatat
    12181 tttctggctc atttgacata aagtaccttc ctataaaccg tcgatctgta aaagtcctac
    12241 cgtgtttccc atctcatttt actatgggag caacactatt ccacaatcgg ttgggctact
    12301 atccaaagac tcaccgctgt ataaaacacc ataagcaata acataggata ctggcttcta
    12361 actgctatag agtgagaggt tgtcttcctt aaagctatga catgagctgt gaggacagaa
    12421 atgatgtggc cgatcacaag agcaaccaca gaaaatatcc atgcaacccg agcatctatt
    12481 atggctatat taaccttaaa atccgatgtg ccgaataaat tccaaccaaa tccataggga
    12541 tcagataaga gcggtattag ttcctgcccg gtgatcaaca gaaaagataa gtagtgagct
    12601 aaattatatg ccaaggctat tgggaccaat gaaaatacaa agctcttcct tgtatcaaca
    12661 gaggtcattt gtccgtgtga cattcgggaa attagcccac acacatataa gtagatcgca
    12721 gcaaaaatta agggtccaag aattatcccg atagtttcta tagtagctgc agccgaacca
    12781 ggtaacgggt ctattgctgg ccataaaact gtctgaattt gaatccacgc aggagtttca
    12841 gaaaacccat caaaggaaac ggtggctaaa gcagtaacat gaaaaagcat aatagccggc
    12901 gtaacacgtt ctccacgaga caatcctaca gaccacggcc gcagacaaaa ttccttattt
    12961 tcagaatcag tattttccca acattcatag caatcgacac aatcaggcaa atttaagttt
    13021 tccttgcaac ctgaagcgca ccgcgtacac cagtttttgg ttccatttat tcttaactca
    13081 gtggcagaaa accgagcgaa taaattaaaa aggacgtgaa agggatcacc atgtgtcaac
    13141 caaacatgtt taccaaacaa aatcatgcct ccccacgtta agagggagta aattagtatt
    13201 agtatcccta aactatacgg ctgagaactc gccggatgca cattctcaat ccaggcaaaa
    13261 agaagaaaga gaaccaacga aggccatgca tcaaattttg cgggccagcc cactaggccc
    13321 atatgtttac cgaatatccg ttcccaatag ttgaatatca ctaaccatgg attcatcact
    13381 agccatatat tcccaacaat acaaacaaca tacccgaccc caacccacca tattatccaa
    13441 acaaaaacag gggcaaaatt ctcaatagcg ttagatgtac ccgtaaggcc agaatgtatg
    13501 gaaataaaaa gaataaatac cgaaattaac ccaaggcacc ttgaaatagc cttacaaaag
    13561 aaagccattg cagaatatcg ataaacatta attcgtgggt attttagatc tgagccactc
    13621 gaccttataa accagccaat aaccacaaat gaaaatgcaa cagtaagagc cgcgccaatt
    13681 aaaaaatagg tcagcgggat aggcaaatca taccgttcac caaatccatg agcgttagct
    13741 gttgagccga ataaaaaaac gcagaccacc aatagaattg ctggtatgaa tttttttgtt
    13801 ctagcattca ccttctttct ctccgatgaa ttttcaacga tatgctcctt tactagaaat
    13861 cacacctttc ccgtttaatg gcatttctcg tgaaagctat ggatatacct ctattgtaat
    13921 tagggtttcc tcttcagatt catgattggc ctggtttgat ggaggtaaac cacttgttaa
    13981 gcgaactttt ttgtctgatt caattttcca ttctatcagt gcaccgggtt ttactatgac
    14041 attatcagga tgaaaataat gacctgcact tcctaccatc accaaaacat taccgctttc
    14101 accatgatca gaggacactt cgatgcttcc accggcatca tgattcatat gatcatgata
    14161 aggaatggtg gtattttcaa tattcatggg cacttggtag gaaaacatct ctcctgaggt
    14221 caatgtttca gactcaaata aggctgcatg ggtatcagca tcaatttcag cccccgaatg
    14281 gtctgaatgt gatccgtgat cctgatgtcc tgtttcgttt ttatgtgagg tgatggcaaa
    14341 cctaccggtg gcatttgcct tgaactccat cacctggtcg ttacctgaga aaactgtttt
    14401 ttcaatatca tatccatgta aatgtactgt aatctccgta tctgaagaaa aatttagcct
    14461 gacattttca ttcttcttaa ttctcaatgt accctcatcc ccaactaatt cgccagaact
    14521 gattccgata tcaaacactg atgtattttc gtctacagcg actgagaaag aattttcatc
    14581 cgaggaacca caacccattg ccaaagcaaa gaaaatagct atccagaaga cctgcattgt
    14641 taaattttta tgattcatta agctttccta attttaaatt tctttctata ttcttttatc
    14701 caagatctca acgacaaact cagaatggct aagaagaaaa gtaatgtccc cactgtcaaa
    14761 attggcacaa ttgggtttgg cttcttaacc tcgacttcat atacaacgga aacctcattg
    14821 ccctctcttc ttacagtcaa cattactaac cacactccct ctttattaaa agatgtgtca
    14881 atctcgtaat acatagggtc cataatgcta tttttcatca gttcgggtcc cacctctggc
    14941 aggtcaatgg ttgaggaaga agatacaaac tctatttcaa catccacgta cctggttcct
    15001 gtctgttcat tttctacaaa agcagcaaaa tgagtgacac ctattgatgg tgaaggtggg
    15061 gtggtgccta acccaattac atattcacca attactttct tttcatacga gcgtatggaa
    15121 ccgttcgctt caagcaaggt gcctgattgg ttatacaaac ctaagaatag aagcgacaca
    15181 aatatagcag cgcggaacca gagcattttt acctccacct tcgaat
//
LOCUS       DASCNS010000093         3702 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000787938, whole genome shotgun sequence.
ACCESSION   DASCNS010000093 DASCNS010000000
VERSION     DASCNS010000093.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000787938"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            64..741
                     /locus_tag="QF838_03460"
     CDS             64..741
                     /locus_tag="QF838_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59781.1"
                     /translation="MLEEAGLVTTSISLVEEHARKIKPPRILSVPFNFGNTLGEVNNP
                     SYQHEILAATFDLLDRSIGPVLELFETDKIERVILSRSEAMTGSEADKKDLLVELSDM
                     KPNYEKWLETNKGRTAVGLSSIDTELFPAVLGFLKQYTAGEVADSGLRPQQFSIGHFV
                     RYCVDDLKSYYLESRIAVKPDASMNDLYQWLWSDTSLGDLLGELAAVMDDSEDKEVKA
                     IAYGIAR"
     gene            925..1584
                     /locus_tag="QF838_03465"
     CDS             925..1584
                     /locus_tag="QF838_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59782.1"
                     /translation="MGNISANKLGGLGLLIGPGFATLIFLVVFMVLGNTGAGDPRDFQ
                     ANFDKQTDLAHLLGLLPGISLIFFLYGINTLYNDVRENGQNEALFRLGNMGVFFGILG
                     IVVSIGLNSATQFDGLSGLNEGYQYTISLIAGAIQSYTGLIFAVGFSLIALAVSSNKE
                     GIHKIFAYVVALIGLINIVVSFINFADASTWEATFIITPITYVVISIWTATLGLDLMK
                     K"
     gene            complement(1764..2627)
                     /locus_tag="QF838_03470"
     CDS             complement(1764..2627)
                     /locus_tag="QF838_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJO59783.1"
                     /translation="MTAEQHHSDEAFVGKQLDPRSFEISKKTVNDYFEGLKVDRSAYE
                     NITEEELVTPSMVIIDAESISGASFRNNFGNLWMRQEVDFHAPLPTEEKLDVRSRVLD
                     IYEWRSRTIVLQESEIFNEDKELLGAMRHHQSYLLDQSSGKVALRKPSDKEGVRKFDV
                     PTGELLEPVSSEIDLEMCGTFFHGNKNYHTDKRASEELGFEEVVVGGKMTISYIGDML
                     DRTFGERWFRGGKLDVKFTNIVWPGDKVTARGVIADGTNSSEGVTEVRVWMEKEDGTV
                     TIVGNAYLPED"
     gene            complement(2656..>3702)
                     /locus_tag="QF838_03475"
     CDS             complement(2656..>3702)
                     /locus_tag="QF838_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO59784.1"
                     /translation="MVLAGSDIWWCDASAQLREFVEKINAPVFLNAMGRGSLHSDHPN
                     LGSLGRRYGLVKSDAVVLIGTPIDFRLSYGDDRLFPQNPKLIEIMMDANKIGQNRAVD
                     VGVVGDVGAIMDQVMESLNDSNYQPKGTAWVEEVMTEDKALKEADVEMMESNASPIHP
                     MRLVKELRDALDEDATVIGDGGDIVTFAARVLNINKPGHWLDPGQFGCLGAGSGFAAA
                     AQLARPGKQVCIVYGDGAFGLTGFDVESFVRFNLPIVCVLGNNGAWNQTTQGVLGRGG
                     RALATFLSQDTDYAKIMEGMGGHSERVTDPNEIKPALERAFASGKPALLDVVIDPDIS
                     YGSMGGRSRQQRQY"
BASE COUNT          978 a          832 c          757 g         1135 t
ORIGIN      
        1 gatgttgttc tgttaattcc gacttgaccg gcttgtacta acgctgtaag cgttatagcg
       61 agagtgttag aagaagctgg tttagtaacc accagtattt ctttagttga agagcatgca
      121 agaaaaatca aacccccaag gatattgtct gttccattta attttggcaa caccttagga
      181 gaagtaaata accctagcta tcaacatgaa atattggcag ccacttttga tcttttggat
      241 agatcaatcg gtccggtatt agaattgttt gagacagata aaatagagag ggtgatactt
      301 tcccgttcag aagcaatgac tggatcggaa gctgataaga aggatctcct agtggaacta
      361 tctgatatga aacccaatta tgaaaaatgg ctggagacaa ataaaggtag aactgctgtg
      421 gggttgagta gtattgacac tgaattattc ccagctgttt tgggattctt gaaacaatat
      481 acggccggag aagttgcaga ttctgggctt cgtccccaac aatttagtat tggacacttt
      541 gttcgttatt gtgtcgatga tctgaagtca tactatcttg aatctcgaat tgctgtgaaa
      601 cctgatgctt ctatgaacga cttgtaccag tggctttggt ccgatacatc tttgggcgat
      661 ctattgggag aactagctgc ggttatggat gactctgagg ataaagaagt aaaagccatc
      721 gcctatggta tcgctaggta ggattttcgg ttcgtgaaat aggcaattgg ttaaagtagc
      781 ccactattgg gtgatattga atatttattt taacaatatg ccgtaaaagt taaggaaatt
      841 ctgcgtgact ctgtcgattt cataccctaa tgtgaatacg cattgcatgg ttacctacgt
      901 ccaccagtca gataaggagt taatatgggt aatatatctg caaataaatt gggaggactc
      961 ggtctcttaa ttggacccgg tttcgcgacc ttaatattcc tagttgtttt catggtcctt
     1021 ggaaacaccg gtgctggaga ccctagagac tttcaagcta actttgataa gcaaaccgac
     1081 ctcgctcatt tactggggtt attaccaggg atatcactga tatttttcct ttatgggatt
     1141 aataccctct ataacgacgt cagagagaat ggacaaaatg aagccctatt ccgtttgggt
     1201 aatatgggtg tgttctttgg aatattaggg attgttgtga gtataggatt aaacagtgct
     1261 actcaatttg atggtttatc aggtctaaat gagggctatc aatacactat tagccttatc
     1321 gccggagcga ttcagtctta cactggcctt attttcgcgg taggtttttc cttgattgcc
     1381 ttggctgtat cctcaaataa agaaggaata cacaaaatat ttgcctatgt ggttgcttta
     1441 atcggattaa taaacatcgt tgttagtttt ataaattttg cagacgcatc cacttgggaa
     1501 gcaactttca taatcacacc gatcacttac gttgtgatat ctatatggac agcaactttg
     1561 gggctggatc tgatgaagaa gtagtaatca aaacacccaa atcggggttt tggaggatca
     1621 ggtccttttt tgtctgattt ttaggtggaa tcaccttggt gcctccaatg caggaaccaa
     1681 tgatttcacc attcaattat tgggttagaa attgtaagat acggggctcc gcatattact
     1741 ggggccccgc aaaaaaagaa aattcaatct tcgggaagat acgcattgcc aacaatggtt
     1801 acggtgccat cttccttctc catccatacc ctgacctcag tgaccccttc cgatgaatta
     1861 gtcccgtccg ctataacgcc ccttgcagtg accttgtcgc caggccacac tatatttgta
     1921 aattttacgt ccaatttgcc gcctctaaac cacctttcgc cgaatgttcg atcaagcatg
     1981 tctccaatat aagatatggt catttttcca ccaacgacaa cttcttcaaa tcccaattct
     2041 tcggacgctc tcttgtcggt atggtaattc ttatttccat ggaaaaatgt tccacacatt
     2101 tcgagatcta tttcactcga gacaggttca agcaattctc ctgtcgggac atcaaatttt
     2161 cgaaccccct ctttgtccga cggctttctt aatgccactt tcccagaact ctgatctagc
     2221 aagtaacttt ggtgatgacg cattgctcct aacaattctt tatcctcgtt gaatatttca
     2281 gattcttgaa gaactatggt tctgctacgc cattcgtaaa tatctaaaac tctcgaacgt
     2341 acatctaact tttcctctgt tggtaaaggt gcatgaaagt caacttcttg gcgcatccac
     2401 agattcccaa aattgtttcg aaagctggct cctgatatcg attctgcatc aatgataacc
     2461 atcgaagggg ttacaagttc ctcctccgta atattttcgt aggccgagcg atcaactttg
     2521 agaccttcaa aatagtcatt gactgtcttt tttgatatct caaaggaacg gggatctaat
     2581 tgttttccaa cgaatgcttc atccgaatgg tgttgttcgg ctgtcatatt tgtacctcct
     2641 tgtcagctgt agtatttaat attgtcgctg ctgccttgaa cggcctccca tacttccgta
     2701 ggaaatatca gggtcaataa ccacgtctaa aagtgcaggt ttacctgaag caaaggctct
     2761 ctcaagggct ggttttattt cgttaggatc agtcactctc tcagaatgtc ctcccattcc
     2821 ttccattatt ttggcgtagt cggtgtcttg tgacaggaat gtggccaatg ctctaccacc
     2881 ccttcctaac acgccttgag ttgtctggtt ccaggctcca ttattaccaa gtacacacac
     2941 gatgggaagg ttaaatcgga caaaagattc tacgtcgaat ccggttagtc caaatgcgcc
     3001 gtctccgtaa actatacaaa cctgcttgcc tggccttgca agctgagcag cggcggcaaa
     3061 tccgcttccg gcccctaggc acccgaattg tccggggtcc aaccagtggc caggcttatt
     3121 tatattcaag accctagcag caaaggtgac tatgtcacca ccatctccaa tgactgtcgc
     3181 atcttcatct aatgcgtccc ttaattcttt tacaagtctc atggggtgta ttggagaagc
     3241 gttactctcc atcatttcta cgtcagcctc ttttaaggct ttatcttctg tcatcacttc
     3301 ttcaacccag gccgtacctt tgggttggta atttgaatca tttagagatt ccattacttg
     3361 gtccatgatc gctcccacat cgcccactac cccaacatcg actgccctgt tttgcccgat
     3421 tttatttgca tccatcatga tttcgattag ctttggattc tgagggaaga gtctatcatc
     3481 accatagctt agcctgaagt ctatgggagt ccctatcaga actacggcat cagatttaac
     3541 taatccgtac cttcgcccaa ggctacctag attcgggtgg tcggaatgta aacttcctct
     3601 acccatcgca ttcaagaaca ccggcgcgtt gattttctct acaaactctc tcaattgtgc
     3661 ggaagcatca caccaccaga tatcagatcc agcaagaacc at
//
LOCUS       DASCNS010000094         4202 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000801073, whole genome shotgun sequence.
ACCESSION   DASCNS010000094 DASCNS010000000
VERSION     DASCNS010000094.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000801073"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="QF838_03480"
     CDS             complement(<1..662)
                     /locus_tag="QF838_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59785.1"
                     /translation="MEKEFNSENLYEELRQSQMDIRHLGDSIQALREELESQNYEKDK
                     NVQSVRMEGVDEVIQLKETITSLREELQNMQFENSQSVERLHSDYAEEMQQLRDTASS
                     LRTELEELKFQKDRDVQKAISDSAIECDQLRATAGALRQELEDLRFNKDNEVQKAITG
                     GADEIMQLKETSASLRDNIETIKSEYDQKLHDIKRDALDEKNHLTETIVTLRNSLEGN
                     SK"
     gene            850..1290
                     /locus_tag="QF838_03485"
     CDS             850..1290
                     /locus_tag="QF838_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59786.1"
                     /translation="MLDYISIVAQTATGIATLAVAVFLASQLKLQHRDSLVATRTELT
                     GGIVQWIGFMITDPGFTHIYLRAIEGDTALNKEERHRFNVFMVSYFLRIQELWDYDNN
                     SDQVTMYANIMLGTGPGVIDWYQNMGRFVMVPRFTKYLDELIQN"
     gene            complement(1320..1505)
                     /locus_tag="QF838_03490"
     CDS             complement(1320..1505)
                     /locus_tag="QF838_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59787.1"
                     /translation="MAVGNPNWIYALPADASTLKSITGLSRSTKILVGEKNQILKRYK
                     MGDWNLSEWIEIFQKAG"
     gene            complement(1888..2016)
                     /locus_tag="QF838_03495"
     CDS             complement(1888..2016)
                     /locus_tag="QF838_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59788.1"
                     /translation="MEGLAVMGFVFGMVGVVALVRLEKLTKALKEKEILDQDYKKE"
     gene            complement(2047..3891)
                     /gene="glmS"
                     /locus_tag="QF838_03500"
     CDS             complement(2047..3891)
                     /gene="glmS"
                     /locus_tag="QF838_03500"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HJO59789.1"
                     /translation="MCGIIGYTGRSQALPILIDGLSKLEYRGYDSAGVSVFNENQTHT
                     TYRSAGKLSELQRLTETSKTDGMSGIGHTRWATHGEATAINAHPHSDTYGNLSVVHNG
                     IVENYLELKEELTKMGHIFSSDTDSETIPHLIEHYMNDGATFEEATGMSVGRIRGANA
                     VVVMCNDQPEKIVAFRTGNAGGLIIGYGKDEMLVASDIPAILSHTNTISHMRPGELAI
                     LEPNSITFRDNHGNSIEKERTVVANDPLSMAKGDYRHFMLKEICEQPDAIIASLMGRL
                     SSDKGLFNLDDFNLSIEEIKKISKIILTGMGTSLHAAMLARLWMERIAGIPAEWDNSS
                     EFRYREPILEKDSLFISISQSGETADTLAAMEEVREKNIAQITLCNYLNTQASHIADQ
                     TLQIRAGLEVAVAGTKTFTCSLVTLYTLAIYFGVQRKHISHNQAASLTEELSRLPEMI
                     GNILVDQRQYEELASRYYTSSDFLFLGRGYSYPVAMEGALKLKEVSYIHAEGYPAGEM
                     KHGPISLINDNMPVVALIPKDSLYEKMLNTVNEVKSRGAIVIAVASESDTEINGKVDH
                     VIRIPDVSDSLSPIVATVPLQLISYYFAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(3896..>4202)
                     /locus_tag="QF838_03505"
     CDS             complement(3896..>4202)
                     /locus_tag="QF838_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59790.1"
                     /translation="LSGSMIKAKPGVARLVQMSYATILPVAITGTEHIGNVVRVVNPT
                     GNIKVNIGSPFSLPKIDGAIDSGILKSMTDMIMERISILLPEKYRGVYEINKKERSI"
BASE COUNT         1066 a          913 c          826 g         1397 t
ORIGIN      
        1 tttttggaat ttccttccaa tgaatttctc aaagttacta ttgtttcagt gagatggttt
       61 ttttcatcga gggcatctcg ctttatatcg tgaagctttt ggtcatattc actcttgatg
      121 gtttcaatgt tatctcttaa ggatgcagat gtttctttta actgcataat ttcatccgct
      181 ccaccagtta tggctttttg cacttcgtta tccttgttga atcgaagatc ttcaagttcc
      241 tgtcttagcg ccccagcagt agcccgcaat tggtcgcatt ctattgcaga atcagaaata
      301 gccttttgaa catcgcggtc tttctggaat ttcaattctt cgagttcagt acgaagactg
      361 ctggctgtat ccctgagttg ttgcatttcc tctgcgtagt cgctgtgtag tctttcaact
      421 gattggctgt tttcaaactg catattttga agttcttctc gcaagctagt gatggtttct
      481 ttcagctgga tcacttcatc aaccccttcc atccggaccg attgaacatt tttgtctttc
      541 tcataatttt gagattccaa ttcttctcgc aatgcctgga tactgtcacc taggtggcgt
      601 atatccattt gcgattgccg gagctcttca taaaggtttt cggaattgaa ttctttctcc
      661 ataatgagct gatattaccg tattgtgtaa ctatctttct agtctaatgt gactggtgcg
      721 ttatatcctc acaaaaattt gaatcgagtc tgacatctca attgtagatg ctccatgtac
      781 catcaccacc agaaaggtgt attttatgtc ctacaacaaa actgcagccc tattggaggt
      841 acgctcagaa tgctcgacta tatatctatc gtagcccaaa cagcgacggg gatagcgacc
      901 ttagctgtcg cagttttcct tgcatcacag ctaaagttgc agcataggga ttctctggtt
      961 gcaactcgga ctgaactgac cggtggtata gttcagtgga ttggtttcat gatcacagat
     1021 cctggcttca cccacatata tttaagagca attgaaggtg atacagcctt gaataaggaa
     1081 gagagacatc gattcaatgt attcatggtg tcgtattttt taagaatcca agagctgtgg
     1141 gattacgaca ataacagcga tcaagtaaca atgtatgcaa atataatgct aggtactgga
     1201 ccgggtgtga ttgactggta tcaaaatatg ggcagatttg taatggtccc ccgcttcacc
     1261 aaatatcttg atgaactgat tcaaaattaa ttggccaagc aatctcagat tgttattttc
     1321 tagcctgcct tttgaaatat ttcaatccat tcagaaaggt tccaatcacc cattttatac
     1381 cttttgagaa tctgattttt ttcacctact agaatttttg tagatcggct caaccctgtg
     1441 atcgacttta atgtgctggc gtcagctggt agagcataaa tccagttggg gtttccgact
     1501 gccattcgtt tgaggtcctt aaatttataa ccgggattca tccctataat gtaaatattt
     1561 gccaaagatg aaagttcttc atcagtgtta gctaatttcc gtaactcgga aatacatgta
     1621 cctcaatcag gggatataaa aagtaagaat tcgggtttgt tatcgactaa aaactgatct
     1681 gaagatattt ttccatggga ggtattcagc tgaaaatacg gcaatttttc aatagaagac
     1741 tcttctgctg cactgtttaa tcgctgtggt actacggtat tttgttcagt ggtacatgct
     1801 aacgcaacga ctacaaataa taagggtaaa aaaatcgatt tattcaacca ttcaccccac
     1861 tgtgactgag tgttttacaa ccggcattta ttctttcttg taatcctgat ctaaaatttc
     1921 tttttctttt agtgcttttg tcaatttctc caaacgaacg agcgccacaa cgcctaccat
     1981 cccgaaaaca aaacccatta ccgctaatcc ttccatgata ccttcacttt aatttactga
     2041 tatgaactac tccaccgtta ctgacttagc caagttccta ggttggtcta catcgcatcc
     2101 tctcctcacc gcaaaataat aagaaattag ttgtagcggt actgtggcca cgatagggga
     2161 aagcgaatcg gaaacatcgg gaatccttat aacatggtcc actttcccgt tgatttctgt
     2221 gtctgattct gaggcaactg caatgactat agcgcctcta ctttttacct cattcacagt
     2281 gttcagcatt ttttcatata gagagtcttt tggtatcaat gccacgacgg gcatgttgtc
     2341 gtttattagt gatattggtc catgtttcat ctctccggcg ggatagcctt cagcatgtat
     2401 ataactgact tcctttaatt taagagcacc ttccatggca acgggatagc tgtaccctct
     2461 cccaagaaat aagaaatctg agctagtgta gtatctactt gccagttcct cgtactgcct
     2521 ttgatcaacc aagatgttcc ctatcatttc tggtaaccgg gaaagttcct ctgtaagact
     2581 ggcggcctga ttatgggaga tatgctttct ttgcactcca aagtatattg caagtgtata
     2641 aagagttacc aaggagcatg taaaggtttt ggtccctgca accgcgactt ctaacccagc
     2701 cctaatttga agtgtttgat ctgctatgtg ggaagcctgt gtatttaggt aattacaaag
     2761 tgttatttgg gcaatatttt tttcccgaac ctcttccatt gctgcaaggg tgtcagctgt
     2821 ctctcccgat tgacttatag atataaaaag tgaatctttt tcaagaatcg gttctcgata
     2881 acggaattct gatgagttat cccattcagc cggtatacct gcgattcgtt ccatccagag
     2941 acgggcaagc atggcagcat gtaagctcgt tcccatccct gtaaggatta tttttgaaat
     3001 ttttttgatt tcttctatgc taaggttaaa atcatcgagg ttgaataagc ctttatcaga
     3061 agataatcgg cccattaatg aggcgattat ggcgtcgggt tgttcgcata tctctttcag
     3121 catgaaatgt ctgtagtcac ctttggccat agagagaggg tcatttgcca cgacagtcct
     3181 ttccttttct attgaattgc catgattgtc tctgaatgtg atggagttcg gctccaatat
     3241 cgcaagttcc cctggccgca tgtgtgagat agtatttgta tggcttagga ttgcgggaat
     3301 gtcacttgcc actaacattt catccttccc atatccgatt ataagacccc ctgcattgcc
     3361 agttctaaac gctactattt tctccggctg gtcattgcac ataacgacta cggcatttgc
     3421 tcctctgatc ctgcctacac tcatcccggt tgcctcctca aaggtggcac cgtcattcat
     3481 atagtgttct attaaatggg gaatagtttc agaatctgta tcagaactga aaatatgccc
     3541 cattttggtc aattcttctt ttaattccag gtagttttct acaatcccat tatgtactac
     3601 agacaagttt ccataagtgt cgctgtgagg gtgggcatta atggcagtag cttcaccatg
     3661 agtagcccat cgtgtgtgtc caatacctga cattccgtca gtctttgaag tttcggttag
     3721 cctttgaagt tcgcttagct tacccgcaga tctataggtt gtgtgggttt ggttttcgtt
     3781 gaaaactgat actccagcac tatcgtagcc tcgatattca agttttgata gcccgtctat
     3841 taagataggt aatgcttgtg agcgtccagt gtagccaata attccgcaca tcaggttata
     3901 tagacctctc tttcttgttg atctcgtaca cccctctata tttttccggt aggagaatag
     3961 aaatcctttc cattatcatg tcagtcatcg attttaagat accactatca atcgccccat
     4021 ctattttagg gagcgagaac gggcttccaa tatttacttt tatatttcct gttgggttca
     4081 ccactcttac tacattaccg atgtgttcag ttccagttat cgctactggc aatatagtcg
     4141 cgtaggacat ttggactagt ctagctactc ccggtttggc tttaatcatg gatcctgata
     4201 ag
//
LOCUS       DASCNS010000095         6816 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000803055, whole genome shotgun sequence.
ACCESSION   DASCNS010000095 DASCNS010000000
VERSION     DASCNS010000095.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000803055"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="QF838_03510"
     CDS             complement(<1..380)
                     /locus_tag="QF838_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59791.1"
                     /translation="MGNKKNVDSIPDVSLTWVQWVQLLVVASGVFLSSLDVSVNVALP
                     RISDYFYASPTTTYLMIIFYLGTTAGLQLTMGRAGDVFGLRKIFILGLVTYSLAMIAI
                     GFSPNIQSVVGFRVLQAVGNSALLA"
     gene            complement(383..1576)
                     /locus_tag="QF838_03515"
     CDS             complement(383..1576)
                     /locus_tag="QF838_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO59792.1"
                     /translation="MNVFYNQKILWPFSNIGDLIIMISKTRMKITDVKLIQLKELEFV
                     GSIEPAWNKGGSMRFSRGGGSFTEVHTDEGVVGIGPGMDPSLVEPVKNVVVGEDPFEI
                     EQIAQRLKYYVHTLPYKGAAGVDVAIWDVIGKASGQPLYKLWGGSKDRMMPYASMILL
                     STPEERAELASSLSEEGWKAIKLRLHHETIQEDIRTVELVRDAVKDSMEIMVDANQAQ
                     SFGKWQPGVTWDFRRAFETATLLGELGVYWLEEPLQRYAFDDLARLNDSVAIPIAGGE
                     NNRGIHEFKTMLENNVYDVLQPESMVLDGITEVRKIGVLSELYGKKIVPHHGGGDIGV
                     IAHLHLVASWPNAPYLELLNDPPIGSYAHKFAIFQNPPIVENGWISLPDSPGLGIEID
                     PAYIE"
     gene            1680..2777
                     /locus_tag="QF838_03520"
     CDS             1680..2777
                     /locus_tag="QF838_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HJO59793.1"
                     /translation="MSGKKIRVGIIGANVGYGWTPRSHAPAIAEMPEIEFSAVCTSRE
                     ETAKEAADTYGVPLAFSNHNEMLDKADLDVVAVVVRVPKHYDLAMDVLKAGKNIYTEW
                     PLGANLKEAEEITSLAKEKGLLTMVGLQSRAAPIFLYLRDLISEGYIGKVLSANMRQF
                     SSGVLSRPPGRTWQKDVELGATTLTIPFGHSIDALCMCLGEFASISGNVSTKVPQWHE
                     QETNRMVEVSSPDTIMITGVLESGTAVSAHVSAIPYHGSGYRFEVYGSEGTLVVTSSE
                     APSTGTSKLWGGQPNGSGLVELEVPKSYTKVPSSVPNGAPFNIAQLWDRFAEGIKTNT
                     EVEPSFSTALTRHKLLDAIQKSSDTGITQKP"
     gene            2792..4120
                     /locus_tag="QF838_03525"
     CDS             2792..4120
                     /locus_tag="QF838_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJO59794.1"
                     /translation="MATLIEQKYIAAHPGSAQRHEKAKHMFPNGVTHDARRQNPFQLY
                     YTHAVGPHKYDVDGNEVIDFWSGHGSLILGHSHPEIVTAVQDQMAKGTHLSGSTDLEI
                     RWGQLVQEMIPSAEKIRFHSSGTEADMMAIRMARAYTEKSKIIKFEDHFHGWSDYLSA
                     DADGIGGIPKEALETVIILPPNDISLVEKTLQDNDDVAAIILEPTGAHMGLEPVLPTF
                     LEALREVTIKFGVVLIFDEVVTGFRISDGGAQGYYGVTPDLTTLAKILGGGLPGGAVC
                     GKAEIINMIEPRDDAYFNKNRRIAHNGTFNANPLSASSGIKALEILKRDPINEKAIGM
                     GNLLKRSLNEVLSKQEIPGCVSGVNSLIHIRLGTDHDCDHEICILSKEDMGKTTNSAR
                     NAQLNLALLNNGVHSGTRFIMSAFHTEEDIAKTAEAFGNALTDVRAEGLI"
     gene            complement(4117..4947)
                     /locus_tag="QF838_03530"
     CDS             complement(4117..4947)
                     /locus_tag="QF838_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO59795.1"
                     /translation="MIGESQNAVQRWRSQVQVHHAQSISAQQNFDPHQDFWKGMAEQF
                     RDDPYRKGDALVDRLEEEFRGCKTLVDIGGGAGRLALPLALTRKKVTVADSSRSMLEE
                     LRKSCEEAKIQNVEPVFTLWEDAVIQVHEGALCSHVTYGIEDIEKFLQNVNRYASKHV
                     VIVAFMKSPQAHLGILWEVVHGEKRVHLPGVPELMDVLWHMGLTPELSVLDHLGPHIY
                     KSDEDALQDLRKRLYVNQETEKDKNLIRAFQTHLKPTGEGLELVNSNGRTSCIISWDN
                     "
     gene            complement(4953..5909)
                     /locus_tag="QF838_03535"
     CDS             complement(4953..5909)
                     /locus_tag="QF838_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59796.1"
                     /translation="MQIGNSNHNYSWHEKWVEAPGSDSVKDGWAHHGIVVSPKTGNVI
                     TFHQSEPKILVFSQDGELVSSWEAGVQNAHGMTLVQEGKDEFLWLADNLSAQVVKVSV
                     GGGHVMSIARPDIETYKSGEKYSPTDVAVYEEKDGGDGTIIVADGYGSSLIHFYNKSG
                     DYRMSIDGTEGEGGRFATPHGIWIDTRKQNHELYIADRSNGQIQVYSPQGRFLRAFGT
                     GAGADWLHSPSGFAAWGEFLIVAELRGSRITLLDVDDEPVAYLGENTGAFKFNEGWPN
                     VPHSTLVPGKFNSPHGIASDGEGNIFVAEWLIGGRINKLTRS"
     gene            complement(5974..>6816)
                     /locus_tag="QF838_03540"
     CDS             complement(5974..>6816)
                     /locus_tag="QF838_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59797.1"
                     /translation="SQEGDLIHSWRGDFTDAHGITVVNEDGNDYLWIADNGSKRLPEF
                     SYEYPPGADNKSGIVLKYDLEGNLLLNLTMPKHDDYQQIRYAPTSIAVDEVRFGGSGD
                     IWVADGYGASLVHKYNAQGEYTLSIDGSTGSGPFSCPHGIVIDRRKPSPELYVADRAN
                     GRVQVFSLNGDYLRTFGEDFFTTPSEFAITNDLMIVAELEARLAILDLDDKLVRYLFP
                     DDLAASSEGWPNEISESGLIERPSRLGPERFNSPHGITVDESGNIYVAEWLIGGRLTK
                     LVLS"
BASE COUNT         1961 a         1635 c         1382 g         1838 t
ORIGIN      
        1 gctaaaaggg ccgaattacc aactgcctgg agtaccctaa accccacaac tgattgaata
       61 tttggagaaa accctattgc aatcattgcc aaggaataag ttactaatcc cagaatgaat
      121 atctttctaa gtccaaaaac gtcccctgct ctccccatag taagttgcaa acccgcagtg
      181 gtacctagat aaaaaattat catcaaataa gtagtggtcg gagatgcgta aaagtaatca
      241 gatattctag gtaaagcaac atttacacta acatcgaggg agctcaagaa caccccagat
      301 gctaccacca gcagttgaac ccattgcacc caagtcaaag aaacatcagg gattgaatct
      361 acattttttt tatttcccaa cactactcta tgtatgcggg atctatttca atacccaggc
      421 ctggagaatc tggtaaggaa atccacccat tttcgacaat cggcgggttt tgaaatattg
      481 cgaatttatg tgcataactg cctattggcg gatcatttag taattcaagg tatggcgcgt
      541 taggccacga tgccactaga tgaagatgcg ctataacccc gatatctcct cctccgtggt
      601 gtggaacgat tttctttccg tagagctctg ataacacccc tatctttcgt acttccgtaa
      661 tcccgtctaa aaccatactc tccggctgca acacatcgta aacattgttt tctagcatcg
      721 tcttaaattc gtggatgcct cggttgttct cgcctccagc tataggaatc gcgaccgagt
      781 cattcaacct agcgaggtca tcaaaagcat atctttgcaa tggttcttcc agccaataaa
      841 cacccaattc tcccagaaga gtagcggttt caaaagcccg gcggaaatcc caagttacac
      901 caggttgcca tttaccaaat gactgagcct ggttagcgtc taccatgatc tccattgaat
      961 ctttgacagc atcgcggacg agctcgacag tgcgaatatc ttcctggata gtctcatgat
     1021 gcaatctgag ttttatggcc ttccatcctt cttcgcttaa tgagctggcc agctctgcac
     1081 gttcctctgg ggttgatagc aaaatcatgc tggcataggg catcatacga tcctttgaac
     1141 ccccccataa cttgtacagc ggttgtccac ttgctttgcc aataacatcc caaattgcca
     1201 catcaactcc ggcagctcct ttgtacggaa gtgtatggac ataatatttg agtctttggg
     1261 caatttgttc aatttcaaaa ggatcttcac ccacaactac attcttcaca ggctcaacaa
     1321 gcgaggggtc cattccaggt ccaataccaa cgactccctc atccgtgtgt acttctgtga
     1381 aagatccacc acccctacta aaacgcatcg aaccaccttt gttccaagca ggctcaatac
     1441 ttcctacaaa ttcaagctct ttaagctgta tcaatttaac atcagtaatt ttcatacggg
     1501 ttttggatat cataataatc aaatctccga tattagaaaa cggccacaga attttttgat
     1561 tatagaatac attcatacct gttgatacca actcctatat actcgacgga aaatagttac
     1621 ttatgggtac taccgataga atcggcacct agataaaggg tcagaaagga taaaaaaata
     1681 tgtccgggaa aaagattcga gtagggatca tcggggctaa tgttggatac ggttggacac
     1741 ccagatccca tgctccggca atagcagaaa tgccggagat agaattttct gctgtgtgta
     1801 cctctcgaga ggaaactgcc aaagaagcag cggataccta tggagtgccg ttagcattca
     1861 gcaaccacaa tgagatgtta gacaaagctg atttggacgt cgtggctgta gtagtgagag
     1921 ttccaaagca ttacgacctt gcaatggatg ttttgaaagc aggcaaaaat atttataccg
     1981 aatggcccct aggtgcaaat ttgaaagagg cagaagaaat cacctctcta gccaaagaaa
     2041 aaggtctttt aactatggtg ggactgcaat cccgcgccgc accgattttt ctatacctca
     2101 gagacctaat aagtgaagga tatatcggaa aggttctgtc agctaatatg cgccagttca
     2161 gttctggggt gttatccagg ccgccgggtc gtacgtggca aaaagatgtg gaattaggag
     2221 caactaccct gacaatacct tttggccact cgatcgatgc tctttgtatg tgcctcggcg
     2281 aattcgccag catttcagga aacgtgtcga ccaaggtccc gcaatggcat gaacaggaaa
     2341 ctaaccgcat ggttgaagtg agctctccgg acacgattat gatcactggg gtcttggaaa
     2401 gtggcaccgc tgtctcggct catgtaagtg ctatcccata ccatggttct ggttaccggt
     2461 ttgaagttta cggcagtgaa ggaacgcttg tggtaacgag cagcgaggct ccctctacgg
     2521 gtacatctaa actttggggg gggcaaccaa atggatctgg tttagtagaa cttgaagtgc
     2581 ctaaatccta caccaaagtt ccgagttcgg tacctaatgg agcccctttc aatattgccc
     2641 agctgtggga ccgtttcgcc gaaggtatta aaactaatac agaagttgaa cccagctttt
     2701 ctacagccct gactaggcac aaacttttag atgcaatcca gaagtcttcc gatacaggaa
     2761 tcacccaaaa accataaggg agttaaacag aatggccaca cttattgaac aaaaatatat
     2821 tgcagcccat ccgggatctg cccaaaggca cgaaaaagca aaacacatgt ttccgaatgg
     2881 tgtcacccac gatgcccgca gacagaatcc gtttcagctt tactacaccc atgctgtggg
     2941 gcctcataag tatgacgttg atggaaatga ggtgatagat ttttggagcg gtcacggttc
     3001 tctcatacta ggacattcac acccggaaat tgtgaccgcg gtccaggatc aaatggcaaa
     3061 aggaactcac ctaagtggtt cgactgatct tgaaatccgt tgggggcaac ttgttcaaga
     3121 gatgatacca agcgctgaaa aaatcagatt tcacagctct gggactgaag ccgacatgat
     3181 ggcgataagg atggcaagag cctacaccga aaaatccaaa ataataaaat ttgaggacca
     3241 ttttcatggc tggagtgatt atttatctgc tgacgctgac ggtatcgggg gtatcccaaa
     3301 ggaagctctt gaaacagtga taatactacc tccaaatgac atttcccttg tagaaaaaac
     3361 tctgcaagat aacgatgatg tcgcggccat tattctagaa ccaaccggag ctcatatggg
     3421 actagagccc gtattgccga cgtttctaga agcactaagg gaggtcacta tcaaatttgg
     3481 tgtcgtacta atttttgatg aagtggttac aggtttcaga atatccgacg gcggcgctca
     3541 aggatattac ggtgtgaccc ccgacttaac aacattggca aaaatattag ggggaggact
     3601 tccaggtggg gccgtgtgtg gaaaagcaga aataattaat atgatagaac caagggatga
     3661 cgcctatttc aacaaaaaca gaagaatcgc ccacaacggg acatttaacg ccaacccgct
     3721 ctctgcatct tcaggcatta aagctttgga aatactaaaa agagacccaa tcaacgaaaa
     3781 agcaatcgga atgggaaacc tccttaaaag aagccttaat gaagtcttat ctaaacaaga
     3841 aattcccggg tgtgtcagtg gtgtgaattc attaatccac attaggttgg gaactgacca
     3901 cgactgtgat catgagatat gtatattatc taaagaagat atgggaaaga cgacaaactc
     3961 cgccagaaat gcacaactaa atctcgcact tttaaataat ggagtccatt cggggactag
     4021 attcataatg agtgcatttc atactgaaga agacatagcc aaaactgcag aagcctttgg
     4081 aaacgcttta actgacgtaa gagcagaagg tttaatttag ttatcccagc taataatgca
     4141 ggaagtccta ccgttggaat ttaccaattc caacccttct ccagtaggtt ttaagtgtgt
     4201 ctgaaaagcc ctgattaggt ttttatcttt ttctgtttct tggtttacat aaagtcgctt
     4261 tcgaagatcc tgcagggcat cttcatcaga tttatatata tgtggaccca aatgatccaa
     4321 gacgctcagt tcaggggtta gacccatatg ccacaaaacg tccattagtt ccggaactcc
     4381 gggtaaatga actcttttct ctccatgaac aacttcccaa aggattccaa gatgcgcttg
     4441 cggggacttc ataaaggcaa ctataacaac gtgtttacta gcatatcggt tcacattttg
     4501 cagaaatttt tctatgtcct ctattccata cgtgacatgg gagcacaagg ctccttcatg
     4561 aacttgtatg accgcatctt cccaaagtgt aaaaactggt tcaacatttt ggattttcgc
     4621 ttcttcacaa ctctttctta gctcttcaag catacttctt gatgaatctg caactgtcac
     4681 tttttttctg gttaaggcca gaggcaacgc tagcctacca gccccaccac cgatatcaac
     4741 caaagtttta catccgcgga attcctcttc aagcctatcg actagagcat caccttttcg
     4801 atagggatca tccctaaatt gctcagccat gcctttccaa aaatcctgat gtggatcaaa
     4861 gttttgttga gcagatatgg attgggcgtg atgaacctga acttgactcc gccacctctg
     4921 aaccgcattt tgagattcac ctatcattaa atttatgatc ttgtcagctt gttaattcta
     4981 ccgccaatta accattcagc tacgaaaata ttaccttcgc cgtctgaagc tatcccgtga
     5041 gggctgttga atttccctgg aaccaaggtg ctgtggggaa cattaggcca tccttcattg
     5101 aatttgaagg ctccggtatt ttctcctaag taggctactg gttcatcatc cacatccagc
     5161 agtgttatcc gtgacccccg caattctgcc acaataagaa actcgcccca ggctgcgaag
     5221 ccactagggc tatggagcca atcagcccca gcaccagtgc caaatgctcg taagaaacga
     5281 ccctggggtg agtaaacttg aatctgacca ttactcctat cggctatata gagttcatga
     5341 ttttgttttc tcgtatctat ccatatgcca tgaggtgttg caaatcgacc accttcacct
     5401 tcagtcccat caatactcat acgatagtcg ccagatttat tataaaaatg gatgagactc
     5461 gacccgtacc catcggccac aataatcgtt ccgtcacctc catctttttc ttcgtacaca
     5521 gcaacgtcag ttggagagta tttttcgcct gatttgtatg tttcaatatc cggccttgca
     5581 attgacatta catgaccccc accaacagaa actttgacta cctgagctga caaattgtcc
     5641 gcaagccaca agaattcatc cttaccttct tgaacaaggg tcattccgtg agcgttttga
     5701 acgcccgctt cccatgaact gactagttct ccatcctgac taaacaccag aatcttaggt
     5761 tcactttggt ggaatgttat gacattcccg gttttagggc ttacgactat tccatgatgg
     5821 gcccagccat ctttcacact atcagaaccc ggcgcctcaa cccatttttc atgccatgaa
     5881 taattatgat ttgaatttcc tatttgcatt ttggggacct ccgccattat atttgatatt
     5941 agttggaatt attaaaggtc gaacggcata cctttagctt aaaactaatt tagttaatct
     6001 tccgccaatt aaccattctg ccacatatat atttcctgat tcatccacag tgattccatg
     6061 ggggctattg aacctttctg ggcccaatct agaaggcctc tcaattagtc cagattcaga
     6121 aatttcattt ggccaccctt cagaggatgc agcaagatcg tctggaaaca aatacctgac
     6181 taatttatca tctaagtcca gtatcgctag tctcgcttca agttcagcca ctatcatcaa
     6241 atcgttggtt atcgcaaatt cactcggggt ggtgaagaaa tcttccccaa atgtccttaa
     6301 ataatctcca ttcagactga aaacttgaac ccttccattg gctctgtcgg cgacataaag
     6361 ttcaggacta ggttttctac gatcgatgac tatcccgtgc ggacaactga aaggccctga
     6421 acccgtggac ccatctattg atagagtata ttcgccctgt gcattatatt tatgaaccag
     6481 gctggcacca taaccatcgg ctacccaaat gtctcctgag ccaccaaacc gaacttcgtc
     6541 aactgctatt gaagtcgggg catatctaat ctgctggtaa tcgtcatgct tgggcatagt
     6601 caggtttaag agcaggttac cttccaaatc atatttaagg actatccccg atttattatc
     6661 tgccccaggt ggatattcgt aggagaactc tggcaacctt ttggaaccgt tgtcggcaat
     6721 ccataaatag tcatttccat cctcattcac caccgtaatc ccatgtgcat ctgtgaaatc
     6781 cccacgccaa gaatgaatca aatcaccttc ctgact
//
LOCUS       DASCNS010000096         3212 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000815516, whole genome shotgun sequence.
ACCESSION   DASCNS010000096 DASCNS010000000
VERSION     DASCNS010000096.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000815516"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            249..569
                     /locus_tag="QF838_03545"
     CDS             249..569
                     /locus_tag="QF838_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59798.1"
                     /translation="MYRIRRVYRTKPGEAGNVAKLVYQQAKIYRDSGHRREFTVSYNG
                     YTLPGEQNIVVLEWFDDAIMSPGREGNNIPKEAMEAGAKYRPLLEAQHIEFYETVDPS
                     LMGD"
     gene            600..992
                     /locus_tag="QF838_03550"
     CDS             600..992
                     /locus_tag="QF838_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJO59799.1"
                     /translation="MPTKFQTSYIPSTHVKAPDGSEIRLFHEVKGGGLSECTLASGLV
                     SSSISHRSVEEIWFFTEGSGLVWRQQGDMEETVEVKPGVSLTIPKHTKFQFRNTGKNR
                     LKFIISTMPPWPGKWEAKPEIGPWPTSF"
     gene            1013..1912
                     /locus_tag="QF838_03555"
     CDS             1013..1912
                     /locus_tag="QF838_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HJO59800.1"
                     /translation="MTSSFDIHSDAFTEILNEDSSVEHIATGFGFTEGPIWVGGCLLF
                     SDIPNSRIVKYDVKEEGASVSTYKYPSGNSNGLTLDHDGNLIACEHTNRRVSITDSNG
                     NIRAVATRYKGKRLNSPNDVVVKSDGWIYFTDPPYGLMNRTQGKELDFNGVFRVSSNG
                     TALELLIKDMTGPNGIAFSPDEKKLYVADSMEKFINVYEIDNDGYPVNGKQFADLDTG
                     EEGVPDGMKIDTEGRVYSTGPGGIWVFSKESQLLGKILMPEIPANCGWGEDGHTLFMT
                     ARTGLYKIRLNSKGTVLTPINKN"
     gene            1932..>3212
                     /locus_tag="QF838_03560"
     CDS             1932..>3212
                     /locus_tag="QF838_03560"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO59801.1"
                     /translation="MTNYDIVINQAHVMDPESGLDQLQNVGISQGKIAMISDSPLNGE
                     QVIDAPGQILCPGFIDLHCHPQDTQIFEVQALDGVTTTLELEVGTSDIEHFYSKWENQ
                     SPINFGASIGHIPVRMKVMNDPGVFLPSGDAGRKLASNPEIVEMQHLIREGLEKGALA
                     VGFGLDYTRGASRWEVIQMFKVAAEFAASCHVHLRGKGHLEPSNAIEALEEVIAAAAV
                     TGAPLHVVHLNSTGMRAVPQLLDMISGANQSGLDVTTECYPYTAGMTKIESALFDDGW
                     QENYGIDYEQLMRPDTGEYLTAKTFSEYRESGGWIITFSTPEESMKAAVKSPLTMIAT
                     DSIIENGKGHPRTAGSYTKVLGEHVRENGDLDLMSALSKMTIMPAKRLQRRAPAFLNK
                     GRIKQGADADIVVFNPLTVKDQSTYTNPIKPPIGL"
BASE COUNT         1048 a          659 c          723 g          782 t
ORIGIN      
        1 ttaggcaaaa tattcccttg cgtgatttcc tcaaggctga gcatgaaggc cagatagagt
       61 cactgactaa agcattgaga gtagggaata tttgtggggc cctcacttgc accaaaaaag
      121 gagataccgc tgcgatgccc accctcgctg aaatcgagag ttttcttaca ttcccttgac
      181 ctgtctatac cctgcaaact tttcgtcagt taacatgtag tcaaaataac caaaaccgga
      241 gttaatcaat gtatagaatc agacgtgttt acagaaccaa gcctggcgaa gccggtaacg
      301 tggcaaaact tgtctatcaa caagcaaaaa tttatcgaga ttcaggtcac aggagggaat
      361 tcaccgtttc atacaatgga tatacccttc caggggagca aaatatcgtc gtactagaat
      421 ggtttgatga tgcaataatg tctccaggta gggaagggaa taacatccct aaggaagcca
      481 tggaagcagg ggccaaatat aggcctctac ttgaagctca acacatagaa ttttatgaaa
      541 ctgttgatcc atctcttatg ggcgattaat cccaaataat tagcaatata gacatacata
      601 tgcctactaa attccaaacc agctatatcc cttcaactca cgttaaagca cccgatggtt
      661 cggaaataag actatttcac gaagtaaaag gaggagggtt aagcgagtgc acccttgcct
      721 ccggattagt ttctagttct atatcccaca gaagtgttga agaaatatgg ttttttactg
      781 aaggttctgg tttggtatgg cgccaacagg gagacatgga ggaaacggta gaggtcaagc
      841 ccggagtatc acttactatc ccaaagcata ctaaattcca atttagaaac actggtaaaa
      901 atcggcttaa atttattatt tccactatgc ctccatggcc aggaaaatgg gaagcaaaac
      961 cagaaatagg cccatggcca acaagctttt aaaataacaa ggagtaccag ccatgacctc
     1021 atcttttgat atccattctg atgcgttcac agaaatactc aatgaggatt cttcggtcga
     1081 acacatagca acaggatttg gatttactga aggaccaata tgggtgggag gatgtctcct
     1141 tttctcagac attccgaata gcagaatcgt taaatatgac gtcaaagagg aaggggcaag
     1201 tgtatctacg tacaaatacc caagtggcaa ttcaaatggc ttaacacttg atcatgacgg
     1261 aaacttaata gcttgcgaac ataccaaccg gcgtgtatcc ataactgact ctaatggcaa
     1321 tataagagct gttgccactc gatacaaagg aaagagatta aatagcccaa acgacgtggt
     1381 tgtgaaatca gacggttgga tctatttcac cgatcctcca tatggtttga tgaataggac
     1441 ccaaggaaaa gaattagatt tcaacggggt ttttagagtc tcttcaaacg gtacggcact
     1501 ggaactattg atcaaagaca tgacaggccc aaacgggatt gctttttcac ccgatgaaaa
     1561 aaaattatat gtggctgatt caatggaaaa atttattaat gtgtacgaga ttgacaatga
     1621 tggctatcca gtcaatggca agcaatttgc agatttggac acgggtgagg aaggtgtccc
     1681 agatggaatg aaaattgata ccgaaggacg ggtttactca actggacccg gtgggatatg
     1741 ggttttctca aaagaatccc aactactagg gaaaatactt atgccagaaa tcccagcaaa
     1801 ttgtggctgg ggagaagatg gtcatacctt attcatgacg gctagaacag gcctatataa
     1861 aattaggcta aattcaaaag gaaccgtatt gactcccata aacaaaaact agaacagtaa
     1921 aagaacacaa catgaccaat tacgacatag ttatcaacca agctcacgta atggacccag
     1981 aatcgggact tgaccaatta caaaatgtgg gtatatcgca agggaaaata gcaatgatca
     2041 gtgactcgcc attaaatggt gagcaagtga tagatgctcc agggcaaata ttatgtccag
     2101 gatttattga tttgcattgt catccacaag atactcaaat ttttgaagtg caagcattag
     2161 atggtgtcac gactaccctc gaacttgaag taggaacaag tgacatagaa catttttatt
     2221 ccaaatggga aaaccagtcc cctattaatt ttggtgcaag cataggccac ataccagtca
     2281 ggatgaaagt aatgaacgat cctggggtat ttctaccctc aggagatgcc ggcagaaaac
     2341 tggccagtaa cccagaaata gtggagatgc agcaccttat acgggaaggc ctagaaaaag
     2401 gtgccctggc cgtaggtttc gggctagatt acacaagagg tgctagtcga tgggaagtca
     2461 tacagatgtt taaagttgca gcagaattcg ctgcctcctg ccatgttcac ctcagaggca
     2521 aaggccattt ggaaccatct aacgctatag aggccctgga ggaagttata gcagctgcag
     2581 cggttacagg ggccccttta catgtggtcc atttaaatag cacgggaatg agggctgttc
     2641 cacaactttt ggatatgatt tcaggagcaa atcaatcggg tctggatgtc accaccgagt
     2701 gctatccata caccgccgga atgacaaaaa tcgaatcagc attatttgac gatggttggc
     2761 aggagaatta cggcatcgac tatgaacaac ttatgcggcc tgatacaggc gaatatttaa
     2821 ctgctaaaac attctccgag tatagggaga gcgggggctg gataataacg ttctcgactc
     2881 ctgaagaatc gatgaaagct gctgtaaaaa gtcccttaac catgattgcg accgatagta
     2941 ttattgagaa tgggaaagga catccgagga cggccggctc ttacaccaag gtacttggag
     3001 aacacgtgag ggaaaacggt gatttagatc taatgtctgc tctttcaaaa atgactatca
     3061 tgcccgctaa aagacttcag cgtcgagctc cagccttctt gaataaaggt agaataaaac
     3121 agggagctga cgccgatata gtagtattca acccgctgac tgtaaaagac caatcgacct
     3181 acacaaaccc tatcaaacca cctataggtt ta
//
LOCUS       DASCNS010000097         2285 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000825972, whole genome shotgun sequence.
ACCESSION   DASCNS010000097 DASCNS010000000
VERSION     DASCNS010000097.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000825972"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="QF838_03565"
     CDS             complement(<1..559)
                     /locus_tag="QF838_03565"
                     /inference="COORDINATES: protein motif:HMM:NF017072.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HJO59802.1"
                     /translation="MIIGVPKEIKSDENRVSLPPDKVEVLVSDGHKVLVESGAGQGSR
                     FSDDSYLYGAGATLVSDPAEIYGTAELIVKVKEPQPNEFDLMQEGQTIFTYLHLAAEL
                     DLLDCILKKNVTGIAYETVEDDKGRLELLHPMSEIAGRLVSQVSARLLTNIENGVGKL
                     LSGVPGSGDCSAFIIGGGTVGFNAAT"
     gene            complement(604..1872)
                     /locus_tag="QF838_03570"
     CDS             complement(604..1872)
                     /locus_tag="QF838_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HJO59803.1"
                     /translation="MVQFEKETIASRWQTADIESDLLSEAARSIGAGSATDAGRFWRL
                     PTEHPDPIVLAGGIPDDTALPTEDLIDSFSKSIRDHFSESLMYGGWFGYEGLRQLIAD
                     RQNRMEGTSLLPDNIIMHNGSSGCLENIGKAFIEPGDIAIVESPSYSGTVRAIRGFRA
                     EVIEVPMDKEGINPDLFKETVESLAAQGKRVKLFYTIPDYHNPMGNVTSLEIRKAILE
                     ICAEHKILIAEDAAYTEVYYTTPPPPSYYALSDGHGVIKMCSFSKILATGLRAGWIQA
                     RDELAEPLTKVRFDMGNSPLIHYALADLIESGKLDSHIDSMRDLYKSKCRTMVDSLKK
                     YCDPYIELDEPEGGYFLWVKCTQTKAFDLIQAAAEEGVVFPLGSIFYVDSSLDDSHFR
                     LAFTRAPFEHLEEAGRRIGKAFEKVLDIQD"
     gene            complement(2005..>2285)
                     /locus_tag="QF838_03575"
     CDS             complement(2005..>2285)
                     /locus_tag="QF838_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJO59804.1"
                     /translation="RHIFPNIIPILAVLLAFQVPAVIFNEAGLSFLGLGIDEPVPSLG
                     KMISTSLGYVRTYWHMGLFPSLMIAIITMGFTFVGDGLRDALDPTMNR"
BASE COUNT          653 a          529 c          450 g          653 t
ORIGIN      
        1 ccgtcgctgc attaaatccc acggttcccc caccaataat aaaagcgctg caatcgccag
       61 atcccggaac accactaagt aatttaccaa ccccgttttc tatatttgtt aaaaggcggg
      121 ccgaaacctg agaaactaaa cgtcctgcaa tttcactcat aggatgaagg agttctagcc
      181 tacctttatc gtcttcaaca gtctcataag cgatcccggt cacatttttc ttcaatatgc
      241 aatcaagtaa atctagctca gccgctaggt ggagatacgt aaaaatggtt tggccttctt
      301 gcattaaatc aaattcgtta ggctgaggtt ctttcacttt aacgatgagc tctgctgtgc
      361 catatatttc tgcaggatcc gaaactaatg ttgcgccggc accatatagg taagaatcat
      421 cggaaaaccg agacccttga ccggcaccag attctacgag tactttatgg ccgtcagaaa
      481 ctaaaacctc gactttgtca ggaggcaaag acaccctgtt ctcatctgat tttatttctt
      541 ttgggacacc tattatcaca atttaaacct tgggccgtga taacgcataa acgctaaaca
      601 cagctaatct tgaatatcta aaaccttttc aaaagctttt ccaattcttc taccggcttc
      661 ctcaagatgt tcaaaaggag cacgggtgaa agctagtcga aaatgcgaat catctagtga
      721 gctatcaaca taaaaaatag atcctagcgg aaaaacaaca ccctcttctg ccgccgcctg
      781 aatcaaatca aaagcctttg tttgagtaca tttgacccac aggaagtaac caccctctgg
      841 ctcgtccaat tcaatataag ggtcgcagta tttcttgagg gaatctacca ttgttctaca
      901 cttggatttg tataaatctc gcatcgaatc aatatggctg tccaactttc ctgactctat
      961 gagatctgct aacgcataat ggattagtgg actgttgccc atgtcgaatc ttactttcgt
     1021 tagtggttca gccaactcat ccctggcttg gatccaacca gccctcaacc ccgtggccaa
     1081 gattttgcta aaactacaca tttttattac cccgtgacca tcagaaagag cataatacga
     1141 tggaggtggg ggggtagtgt aataaacttc agtgtatgcg gcatcttcag ctatgagaat
     1201 tttgtgttct gcgcatattt ccagaattgc ttttctaatt tctaatgaag tcacattacc
     1261 cattgggttg tgataatcag gaatggtgta aaagagtttt actcttttac cttgggctgc
     1321 aagcgattcg acagtttcct taaacaaatc gggattaata ccctctttat ccatgggcac
     1381 ttcaataact tctgctctga accccctaat ggctcttacc gttccagagt aacttggaga
     1441 ttctacaatg gcgatgtctc caggctctat aaaagccttg ccgatgtttt ctagacaccc
     1501 ggagcttcca ttgtgcatga tgatgttgtc aggtagaagg cttgtccctt ccattctatt
     1561 ttggcggtca gctattaatt gccttaggcc ttcatagcca aaccagcctc cgtacattaa
     1621 agattcactg aaatgatctc taattgattt agagaaacta tcaatcaagt cttctgttgg
     1681 aagagcagta tcgtctggaa ttccaccagc taaaacgatg gggtctgggt gttctgtggg
     1741 gagtctccaa aaacgaccag catcagttgc gcttccagca cctatgctgc gggcagcttc
     1801 tgaaagcaag tcgctttcta tgtctgcagt ttgccatcgg ctggcgattg tttctttttc
     1861 gaattgcacc atatcagtaa accttatgcc tattgatgtc ttaggcgaat aggacaggta
     1921 atcgttttat ttcaataaag gataacctgt caagactaac tagtgaagcc ctaaaatgat
     1981 agataaaaac aaagagtaaa aggcctatcg gttcattgta gggtctaagg catctctcaa
     2041 accgtcacct acaaacgtaa agcccatcgt aataattgct atcataaggc ttggaaatag
     2101 acccatatgc cagtaggttc ggacatagcc aagactggtt gatatcattt tgcctaaact
     2161 tggcaccggc tcatcaattc caagtcctaa aaagctaagt ccggcctcat taaaaatcac
     2221 agctggaact tggaaggcca gcaggacagc taatatgggt atgatatttg ggaaaatatg
     2281 tctaa
//
LOCUS       DASCNS010000098         8222 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000827431, whole genome shotgun sequence.
ACCESSION   DASCNS010000098 DASCNS010000000
VERSION     DASCNS010000098.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000827431"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="QF838_03580"
     CDS             complement(<1..941)
                     /locus_tag="QF838_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59805.1"
                     /translation="MKLPTIHTLDSLRAYPDYRYLWTANFCANTAQWLQLLTLGWLVK
                     FLTEDGSNSALLVVGVGGAAALPGIIIGPWGGVLGDRLNRKKLVISLEIFMVFLALSF
                     ALLLQAEVVLVWHVYTFALLAGCCEALKMPIRQALIANTVPFHALSNAYATSVLTIPG
                     TRMIGPFIGGIIVSNVGFFWNFAIEALLYLGVIVALIPMSTPYFIKREFVTGFGMSQM
                     VTDMVDGFKYLWKQQRPLSLIYMLSAAPNVVLHPVIFLLPLFTTDVLHRDVDYGGYMM
                     AVNGAGGLLMVFVLFVVGLGGCCGLWVSGFVIGVVWLV"
     gene            complement(1015..2115)
                     /locus_tag="QF838_03585"
     CDS             complement(1015..2115)
                     /locus_tag="QF838_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO59806.1"
                     /translation="MKITNVKVEMFNWTTEAWKTGVGTSFGNTRQLGIVTVETDEGIS
                     GNSFLGSSRVGADHFAPGLIEFIKPLVMGRNPQDIGAIWWDMWKMNRSVSTNVIGAID
                     ICLWDINGKIAQQPIHRLLGTCKESVPVYSSTAFHDTKEEYAEEAIKFKEMGWTAHKI
                     HPHGNPTYDIEISQAVREAVGDDMKLMLDSMWAYQYEDAMRVGRAIEELNFYWYEDPL
                     VEEDIYNYVKLHDKLDIPIMSTEYAPGRYYGMTQWITQYATDILRGDVAVTGGITPMV
                     RLCHLAEGFNMKCEIHHGGNSLNNVANLHITMAVPNCEFFEFFPCTGANMFGLVEDIQ
                     FEGGVVHAPTKPGLGYEIDWDMLKNEYTATAE"
     gene            2242..2910
                     /locus_tag="QF838_03590"
     CDS             2242..2910
                     /locus_tag="QF838_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HJO59807.1"
                     /translation="MNARLIPLPNPKHFSNEEMESRSTEFYLNIKQRRTVRDFSSEAV
                     SRSIIDNCILAAGTAPSGANMQPWHFAVSGQGEARKIIREKAEKEEKDFYETKASPEW
                     LKALEPLGTDPNKPFLETAPFLIGIFVKSYGILPDGSQVKHYYATESVGIATGILITA
                     LHNAGLSTLTHTPSPMGFLNKIFNRPKNERPFLLLVAGYAEKGAQVPDIHKMSLGEIS
                     SEIS"
     gene            complement(2918..4336)
                     /locus_tag="QF838_03595"
     CDS             complement(2918..4336)
                     /locus_tag="QF838_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HJO59808.1"
                     /translation="MSKSEIVNMTATEMVGLIKAKDISAAEVMDAHLMQINSVNPKVN
                     AIVTIHPELGIEGAKLADKAIAKGENLGLLHGLPTAHKDLVPTKGIRTTYGSPLYADY
                     IPDYSALIVERMQKAGAITIGKTNTPEFGAGSQTYNEVFGETLNPYDTNTTCGGSSGG
                     AAVALATRMVPIADGSDTGGSLRNPANFCNVVGFRTSPGRVPTYPSKVGWSPTSVQGP
                     MARTVSDCALFLAAIAGPDSRVPMSITEKGEVFLEPLNRDFKNIKIAWSQDLGGLPID
                     PRTTAVIESQRSVFKDIGCLIQEAEPDFSDVNEQFLIWRGWGREINQGDNLRNNRDKL
                     KDTMIWDIEQGVKLSGPDVGWAEAKRMELFQRMNQFMDEYEFLICPVSQRPPFNIEER
                     WVSEINGVKMENFIAWMKSCYYITATGHPAISVPCGFTPEGLPVGVQIVGRYRDEFGV
                     LQLAKAFEDATGFWKSIPAVCT"
     gene            complement(4353..5672)
                     /locus_tag="QF838_03600"
     CDS             complement(4353..5672)
                     /locus_tag="QF838_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59809.1"
                     /translation="MIQDKLGTSGAKTFYWHYAWVVVVIIAGMQIVGTSIRMAFGVFI
                     DPLQTQFGWSQGGIGFAYAVCSIVSAVASPWAGNLGDRFGAKKSMLAGTFVFLIGMLS
                     TAFVKELWQFWLTYGVILGIAQAIFLVPLIPAAMIWFRRHLGLAMGLIMASWGVGPAL
                     ATPLVAFMIQTFGWTFAFVSIGIVSTLIMLALVSVFKNRPADVGGLAYGTFKSDPPLS
                     DKLPPSILLDEFRGHMRKTAAYWNMSSIHFLGCVGHAVILIWIIPMATSRGMSPIDAS
                     LILTLFSAVSIVTRLTTPVLCERLGVRTVMTFFYILQGAPVLLLIFSSMDGVFLIFAV
                     SFGIGYGGETGGFPILNRKYFGHAPMGGAHGFQMLGAGIGMALGGWVGGPVFDIFDSY
                     NAAFAISVVASLAGAISIILLENPAKLLIPDWQKSEEEFEQNLKITT"
     gene            6088..6879
                     /locus_tag="QF838_03605"
     CDS             6088..6879
                     /locus_tag="QF838_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO59810.1"
                     /translation="MVRPNLIRQKLKSEEPTLSTHIHSVWPAEIEAIGHAGIYDYVEF
                     VAEYGPSDLHDLDNMCRTSELYNLGSMIKIDQSLMPFLAQRGIGSGYESVLFTDVRNI
                     EEVRECIKSARPDHPDHGGLYGVATRRNSYMGYGGTQEYVDMLGDVVLAFMIEKKGAV
                     DNLEEILSEPGVEMIQWGGADFSMNIGKPRQMNDPEVQAAKKKTFELGIKMGVPPRAE
                     IQSADEMKEYLDMGVRDFSLGTDISILYSFWKNEGEKVLRAMEGE"
     gene            6889..7683
                     /locus_tag="QF838_03610"
     CDS             6889..7683
                     /locus_tag="QF838_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59811.1"
                     /translation="MELSISGKKAIITGGSKGIGKAIARELAKEGVDLGLCARNKSDL
                     IETADEITSNYDVKVFTAVADTRDDSSISMFIDGAASNLGGLDILINNAAAPGGLVMG
                     PLDQADPLELIKDIDTKVVGYLRSTKKSVPYMKKGQWGRIINIGGLAARTAGAISGMR
                     NLALVHLTKTLSNELGQFGITANIIHPGATRTERTQPMQENIAHTQGLSVEDIEKKAT
                     QFIDIQRMVEAEEIASLAVFLCSPRASAITGESIAAGGGVGTAVFS"
     gene            7718..>8222
                     /locus_tag="QF838_03615"
     CDS             7718..>8222
                     /locus_tag="QF838_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013866.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HJO59812.1"
                     /translation="MYTTEQFEGIIAETITINGTNGDAIHTYFARPMGAGPFPAVVLA
                     HHLPGWDELYREFTRKLAHHGYLAISSDLYCREAHGTPEDVAAAVRAEGGVSDDQVMG
                     DLEAAAKYVLSLPSSNGKVGTFGTCSGGRHVFLAGCRLDCFDAIVECWGGNVIMSDEQ
                     LTDKQPVS"
BASE COUNT         2563 a         1907 c         1655 g         2097 t
ORIGIN      
        1 accaaccaaa caaccccaat cacaaatccc gagacccaca acccgcaaca accccccaac
       61 ccaacaacaa acaacacaaa aaccattagc aacccaccag ctccgttaac tgccatcata
      121 taaccgccat aatcaacatc tctatgaagg acatctgtgg tgaacaaagg caacaaaaat
      181 atgacaggat gcaatacaac atttggtgcg gccgacaaca tataaatcaa tgacaaggga
      241 cgctgttgtt tccacagata cttaaaccca tccaccatat cggtaaccat ctgtgacatt
      301 ccaaaacctg taacaaattc tcgttttata aaatagggag tggacatcgg gattagagcc
      361 actataaccc ctagatacag gagtgcctcg atagcgaaat tccaaaaaaa tcccacattc
      421 gagacaatga taccgccaat gaagggacca atcattcgtg tgccaggtat cgtgagaaca
      481 cttgtagcat atgcgttgct aagggcatgg aagggaacgg tattagcgat aagagcttgt
      541 cggatcggca tttttagtgc ttcgcagcac cctgccagaa gtgcaaatgt atacacatgc
      601 cacacaagta cgacttctgc ctgaagcaac aaagcgaagg aaagagcgag gaaaaccata
      661 aatatttcga gggaaataac cagcttttta cgatttaatc tatcacctaa aactccaccc
      721 caagggccaa taatgatccc gggtaaagca gcagcaccac ctacccctac tacaagcaag
      781 gcagaattag agccatcctc agtaagaaat tttactaacc atcctaaggt gagtaactgc
      841 aaccattgtg cagtatttgc acaaaaattt gccgtccaca ggtatcgata atcaggatag
      901 gcccgcaatg aatctaaggt gtgtatagtg ggtaatttca tacccgtgca aacgcgatca
      961 aaagcttttc caaaatagat gaaatcttaa ttttaatggg accgacaggc ttttctattc
     1021 agctgtagct gtgtattcgt tttttagcat atcccaatca atctcatatc caagccccgg
     1081 tttagtcgga gcatgaacca caccaccttc aaattgaata tcctctacaa gcccaaacat
     1141 attggctcct gtacatggga aaaattcgaa aaattcacag ttaggtacag ccatagttat
     1201 gtgcaaattc gccacattgt ttaacgaatt accaccatgg tgaatctcac atttcatatt
     1261 aaacccttca gcaagatggc aaagccgaac catcggggta attcctccag tcacagccac
     1321 atctcccctt agtatgtcag ttgcatattg ggttatccac tgggtcattc cgtagtatct
     1381 gcccggagca tattctgtgg acataatcgg tatgtctagc ttatcgtgca atttcacata
     1441 gttataaata tcttcttcta ccaaaggatc ctcataccag taaaaattca gttcttcaat
     1501 agcccttccc actcgcattg catcttcata ttggtaggcc cacatagaat ccaacatgag
     1561 tttcatatca tctccaacgg cttcccgcac agcctgcgat atttcaatgt cataagtagg
     1621 attcccgtgt ggatggatct tgtgtgcagt ccaacccatc tctttaaatt tgattgcctc
     1681 ttccgcatat tcttcttttg tgtcgtgaaa tgcggtgctg gagtaaacag ggactgattc
     1741 tttacaggtt cctaatagcc gatgtatggg ttgttgggct attttcccat ttatatccca
     1801 cagacaaata tcgatagccc caataacatt cgtagacaca gaccgattca tcttccacat
     1861 atcccaccaa atggcaccga tatcctgagg gttccgaccc ataaccaatg gtttaataaa
     1921 ttctattaat cctggggcaa aatgatccgc cccaaccctc gaagaaccca gaaacgaatt
     1981 gccacttata ccttcatctg tctcaacagt aacgatccca agttggcgtg tgttaccaaa
     2041 tgaagtaccc acaccagtct tccaagcttc cgtagtccaa ttgaacattt caactttaac
     2101 attcgttatt ttcataatat gccttttaca tctggattat ccttgcctgt ttcacctagg
     2161 ataacatcag cgggatgaga aatcgtacag gatgacaaaa gaatctaaga attaccagaa
     2221 gctaattcaa tggaggacaa agtgaatgca agattaattc cattacctaa ccctaaacat
     2281 tttagtaatg aagagatgga atctagatct acggaattct atctgaacat caagcagcgc
     2341 cgaaccgtgc gagatttttc ttccgaagca gtatctaggt ccataatcga taattgcatt
     2401 ttagccgcgg gaacagctcc aagtggagca aatatgcaac cttggcattt cgctgtttca
     2461 ggccagggag aggctcgcaa aatcattcgg gaaaaagctg agaaagaaga gaaggacttc
     2521 tatgaaacaa aagcgtcacc tgaatggtta aaggccttgg aaccgttagg gacagatccc
     2581 aataaacctt tcctagaaac cgccccattc ctgattggca tatttgttaa aagctacggg
     2641 atactgcctg acgggtccca ggtaaaacac tattatgcaa cagaatcagt gggtatagct
     2701 actggcattc taataaccgc tctgcacaac gcagggctgt ctactcttac gcataccccc
     2761 agccctatgg gatttttaaa caaaatattt aatcgcccaa aaaacgaacg cccattctta
     2821 ctcctcgtgg caggttacgc agaaaaagga gctcaagtgc cggatatcca taagatgtca
     2881 cttggagaga tctcatctga gataagttga aaccatttta agtgcagaca gcagggattg
     2941 atttccaaaa cccagtggcg tcttcaaaag ctttagcaag ttgtaagacc ccaaattcat
     3001 ccctatatcg acctacgatc tgaactccga ctggaagacc ttcaggggtg aatccgcaag
     3061 gaaccgaaat agccggatgt cctgtagctg ttatgtaata acaagatttc atccaagcga
     3121 taaaattttc cattttgacg ccatttattt cagaaaccca tctttcttca atattaaaag
     3181 ggggtctttg gctcacaggg catatgagaa attcatattc gtccataaac tggttcatgc
     3241 gttgaaacaa ttccattctt tttgcttcgg cccatcctac atctggccca gataatttga
     3301 ccccctgctc aatatcccaa atcatcgtat ctttcaattt atctctgttg ttccttaaat
     3361 tatctccctg gtttatttct cttccccatc cccgccaaat caaaaactgt tcatttacat
     3421 cggaaaaatc cggctccgct tcctgaatca agcatcctat atctttaaaa actgacctct
     3481 gagattcaat tactgcggtg gttcggggat cgatcggtaa tcctcctaaa tcttgactcc
     3541 atgcgatttt aatattctta aagtccctat taagaggttc caaaaaaact tcaccctttt
     3601 cagttattga catgggaacc ctagaatcag gtccagctat agcggccaga aaaagggcac
     3661 agtcagaaac agttctagcc atagggcctt gtactgaagt tggactccaa cccaccttag
     3721 atgggtaagt aggtactcga ccaggagatg tcctaaatcc gaccacatta caaaagtttg
     3781 ctgggttacg tagggagcct ccggtatcac ttccatcagc aatcggaacc attctagtag
     3841 cgagagccac agctgccccg ccgctgctgc ctccgcaggt cgtgttggta tcgtaagggt
     3901 tcaaagtttc tccaaatacc tcattgtaag tttgggaccc tgccccaaat tccggcgtat
     3961 tggtcttacc aatcgtgata gccccagcct tctgcatcct ctccactatt aaggcgctgt
     4021 agtcaggtat gtagtcagca tacaaaggag agccgtatgt tgtccgaata cctttagtcg
     4081 ggactaaatc cttatgggca gtaggtagtc cgtgtaatag acctaaattt tctcctttgg
     4141 caattgcttt atctgcgagt ttggccccct caattcctaa ctcgggatga atagtaacaa
     4201 tggcgttgac tttaggattt acagaattta tttgcattag gtgggcatcc atcacctctg
     4261 cggctgaaat atccttagct ttaattaacc caaccatttc agtggcagtc atgttaacta
     4321 tttcagattt agacatatga ataactccat tctcacgtgg tgattttcaa attttgttca
     4381 aattcctctt ccgatttttg ccaatccggt atcaatagtt tggcagggtt ctctagcaaa
     4441 ataatactaa tagctcctgc taaactcgct actacactaa tagcaaatgc agcattgtat
     4501 gaatcaaaaa tatcaaacac tggacctcca acccaacctc ctaatgccat acctatgcca
     4561 gctcccagca tttgaaaccc atgggctcct cccataggtg catgcccgaa atatttccta
     4621 ttaagaattg ggaaaccccc agtttctcct ccatatccaa tcccaaaact gacagcaaaa
     4681 attagaaaaa ctccatccat cgatgagaat atcaaaagta ataccggtgc accctgcaaa
     4741 atgtaaaaga atgtcataac ggtccgcacg cccaaccttt cacataaaac cggggtagtt
     4801 aatctagtga ctatgctgac tgcagaaaac aaggtcagaa ttaacgaagc atctattgga
     4861 ctcatacctc tgctagtggc cattgggatt atccaaatca agatcactgc atgccccacg
     4921 caaccaagga aatggataga gctcatattc caataagctg cagtttttct catgtgtccc
     4981 ctgaactcat caagaaggat agaaggagga agtttgtcac taagtggtgg atcactttta
     5041 aaagtaccgt atgccaaacc ccctacatct gccggcctgt ttttaaatac agaaaccaag
     5101 gccaacataa ttaatgtcga gacaatccct atagaaacaa aagcaaaagt ccatccaaat
     5161 gtctggatca taaaagctac aagaggggtg gccaatgcag gccctactcc ccaggaagcc
     5221 ataatgaggc ccatagccaa acctaggtgc cttctgaacc agatcatagc cgcagggata
     5281 agtggtacaa gaaaaattgc ctgagcaata ccaagaatga ccccatatgt aagccaaaat
     5341 tgccataact cttttacaaa tgcagtactc aacataccga ttaagaaaac aaatgttccg
     5401 gcaagcatcg atttttttgc cccaaaccta tcccccaagt tacctgccca aggagatgca
     5461 acagccgaca ctatgctaca aactgcatag gcaaaaccaa taccgccttg actccatcca
     5521 aattgagttt gcaatggatc gataaacacc ccaaaagcca ttctgatgga tgtccctact
     5581 atctgcatcc cagcaataat gaccactacg acccaggcat aatgccagta aaaggttttt
     5641 gccccagaag tccctaattt atcttgaatc atttttaaaa gaattctcca acctcagtga
     5701 agaggacaca tccaaagcgc tgtcgattcc caagcataaa tttctgtttg ccctgcctca
     5761 gatcgaacaa ctttccgttt ttctatccga gtcgaagctg acatggcgag cacataaatg
     5821 actagcaacc ccgtaacaaa aataacaaag ataaattgta ccggcagtcc caaaataaaa
     5881 gtgttcttta acaattactc actacattta agaaagaatg gaaggcagtt tcatgaaatt
     5941 tatgtgagaa ttatattctg taactgtctt ccgtgactag gttaaaaatg ttgtgtttac
     6001 agccttaatt ttaaacagat gaattgtggg taatctttct gataacctac cgataccata
     6061 ggaaaaacaa atatcacgga ggatgcaatg gttaggccta acttaatcag acaaaaatta
     6121 aagtcggagg aacccaccct tagtactcat atacacagtg tgtggccggc agaaatagaa
     6181 gccattgggc atgcaggtat atatgactat gttgaattcg tggccgagta tgggccatca
     6241 gacttacacg acctcgacaa catgtgccga acatctgaac tctacaatct tggcagcatg
     6301 attaagatag atcaaagcct tatgcccttc cttgctcaaa gaggaatagg ttctggttac
     6361 gaaagtgtgc tttttacaga cgtacgaaac atagaagaag tccgggaatg catcaaatca
     6421 gctaggcccg accatcctga tcatggaggc ctatatggtg ttgccaccag aagaaactcc
     6481 tacatgggat atggtggtac gcaagaatat gtagacatgc ttggggatgt agtattggcc
     6541 ttcatgattg aaaaaaaagg cgcagttgac aacctggaag aaattttatc tgaaccgggt
     6601 gtggagatga ttcaatgggg cggtgcggat ttttcaatga acattggtaa accaagacag
     6661 atgaacgacc ctgaagtgca agcagcaaag aagaagactt tcgaattagg tatcaaaatg
     6721 ggagttcctc caagagctga aatacaatcg gccgacgaaa tgaaagagta tctagatatg
     6781 ggagtaagag atttctcatt aggtacagac atatcaatac tttactcatt ttggaagaac
     6841 gaaggggaga aagtactgag ggcaatggaa ggggagtagt taactgtcgt ggaactttcc
     6901 ataagcggta aaaaagcaat aatcaccggc ggtagcaaag gaatcgggaa agctattgca
     6961 agggaattgg ccaaagaagg cgttgatttg gggttgtgcg caagaaacaa atcagactta
     7021 atagaaaccg ctgacgagat aacttccaat tatgatgtga aggtttttac tgcagttgca
     7081 gatactcgag atgatagctc gatttccatg tttatagatg gggccgcttc taatcttggg
     7141 gggctggata ttctaataaa caatgccgcc gccccaggtg ggttggtcat gggtccacta
     7201 gaccaggctg acccattaga acttataaag gacatagata ccaaagttgt tggatactta
     7261 cgatcgacaa aaaagtctgt gccttatatg aaaaaaggac aatggggcag aataatcaat
     7321 attgggggtt tggcggcaag aactgcagga gctatcagtg gcatgcgaaa cctagcccta
     7381 gtccacctaa caaaaactct ctccaatgaa ttaggccaat ttggcataac ggccaatatc
     7441 attcatccag gtgccaccag aacagaaaga acgcaaccaa tgcaagaaaa catcgcgcat
     7501 acccaaggct tatccgttga ggatatcgaa aaaaaggcta cacaatttat tgatatacaa
     7561 agaatggtgg aggctgaaga gatagcctct ctagcggtat ttttatgttc tccgagagcc
     7621 tccgctataa ctggtgaatc gatagccgcg ggtggtggtg taggtacagc cgtgttttcc
     7681 taataatcaa tccatcaatc aaattagagg agtaaaaatg tatacaacag aacaattcga
     7741 gggaatcatc gcagaaacta tcactattaa tgggacaaac ggggacgcaa tacatacgta
     7801 ttttgcgaga ccaatggggg caggcccgtt tccagccgta gttttggctc accacttgcc
     7861 agggtgggat gaattatatc gcgaatttac acgcaaattg gcgcatcatg gatacctagc
     7921 tatatcgtca gatctttatt gcagggaggc acacggtacc cccgaagacg ttgcagcagc
     7981 agtaagagca gagggcggag tatcagatga tcaagtcatg ggtgatttgg aagctgcagc
     8041 caaatacgtt ctgtctctgc cgagttcaaa tggtaaagtc gggacatttg gaacatgctc
     8101 aggaggtaga cacgtatttc tagccgggtg tcgactcgac tgttttgatg ccatagtgga
     8161 atgttggggt ggaaacgtca ttatgtctga cgagcaatta acagataagc aacctgtctc
     8221 tc
//
LOCUS       DASCNS010000099         3270 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000833862, whole genome shotgun sequence.
ACCESSION   DASCNS010000099 DASCNS010000000
VERSION     DASCNS010000099.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000833862"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="QF838_03620"
     CDS             complement(<1..952)
                     /locus_tag="QF838_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HJO59813.1"
                     /translation="MPGKKIAPYGSWESPITTGSIVSHSINIGDLFISNEGKFWQEMR
                     PIEGGRSTIMCQTSDGCKREIIPKSYNARTRVHEYGGGSFLVHDDGVYFSNFSDQQIH
                     KVNLTGGNPTQITNEPLLRFADGAVDAERQQIIYVGENHDSIDEPVNCIVSVDLQNNG
                     EVTILASGADFYASPVISPDGRKLVWVQWNHPNMPWDSTELYTADLSHSKLHNIQKIA
                     GNGESICQPLWSPNGILHFVSDLSGWWNIYKYTNGESYNLTPIDAEFTVAQWGLGTRF
                     YGFIDGNQIICSYNSLGFWKIGLLDSLSSQFSDLKLNVNIT"
     gene            complement(961..2082)
                     /locus_tag="QF838_03625"
     CDS             complement(961..2082)
                     /locus_tag="QF838_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007256847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59814.1"
                     /translation="MTRVSIHGEKWLIDGIPTYKGITYQGINIEGLLLNARMVNAIFD
                     DDNPYTRHIWKYPDTTEWDPDRNTNELIRMLPDYKKYGLTAITVNLQGGAPSGYYRLN
                     QFREFMKQRGISGKDSEIWKGLPSPESQPWNSSGFNPDGSLKSDYIRRLRKIIEETDR
                     LSMVLILGLFYFGQDERVRDEASVISAVRNTCEWLLTSGYDNVVIEINNECSVPKYEH
                     DILQPHRVHELIELVKSIKSESKRLLVGTSYGGNTIPENNVVKSSDFVLMHGNGVVDP
                     KRISEMIEETRALTEWHEMPILFNEDDHFDFNHELNNFYCAIRSSAGWGYFDPGDGAG
                     GNAAFGDYRNGYQLIPINWAINTDRKKSYFNYLAAVTSR"
     gene            complement(2090..>3270)
                     /locus_tag="QF838_03630"
     CDS             complement(2090..>3270)
                     /locus_tag="QF838_03630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO59815.1"
                     /translation="ITPPPNAGGYPDDDPGKEPWNRQSNFNAINRNKKSLTLDLKTPQ
                     GLTSFKDLVSQSDVVFCNYARTVMENFGLSYEQVRKIKPDIIYMLMPGYGNDGPYANY
                     RSMGMAIDAITGHSFLRGYPDLNHSSNSLVHHPDAVAATNGVMALTSALVYRAKTGKG
                     QFIDLSQAESFMTHLGEIYLENQMTSSIRKRRGNRHPDFAPQGVYKCSGDDNWIAISI
                     KTEKEWCELKTIVGDDSLGETGLDSLEGRLKNHDHLDNVINKWASTRDRYDALTLLQA
                     AGITSGVVIDCGADSYADPHLNERDFFQIVDHPAAGLYPMTGPIFKFESDHGRVTHNP
                     APCLGQNNESILEELLGYSRETINKFKTDEIIGTVPLPGSDMGGSRRVAKQKEENSKD
                     "
BASE COUNT          856 a          708 c          663 g         1043 t
ORIGIN      
        1 ccgtgatatt aacgttgagc tttaggtcag aaaattgtga ggaaagtgag tccaatagcc
       61 ctatcttcca aaaccctaga ctgttgtatg agcatattat ttggttgccg tctataaaac
      121 cataaaatct agtgcccaaa ccccattgag caacagtaaa ttcagcgtct atgggggtta
      181 aattatatga ttccccattg gtgtatttgt aaatgttcca ccaaccggaa agatccgaaa
      241 cgaaatgcaa aataccattg ggagaccata gcggctgaca aatagactcc ccgttcccgg
      301 cgattttctg gatattgtga agttttgaat gggataaatc agccgtataa agttctgtag
      361 agtcccaagg catattaggg tgattccact gaacccaaac aagtttgcga ccatcagggc
      421 tgatcaccgg agaagcgtag aaatcagcgc cggaggccag aattgttacc tctccattat
      481 tttgtagatc aactgaaact atgcaattga caggttcatc aatgctgtca tggttttcac
      541 caacataaat aatttgttgc ctttctgcat ctactgctcc atcagcgaat cttagaagcg
      601 gttcatttgt gatttgagta ggattaccac cagtcaggtt aactttatgt atttgttggt
      661 ctgagaaatt ggagaaatat actccatcgt catgtactag gaaagaccct ccgccatatt
      721 cgtgaactcg tgttctcgca ttgtacgatt taggaataat ttcacgttta caaccatcgg
      781 aagtctggca cattatcgtc gaccggcctc cttctatcgg tctcatttct tgccaaaatt
      841 tcccctcatt agatatgaat aaatctccga tatttattga gtgagagaca attgacccag
      901 ttgtaatcgg agattcccat gagccataag gagcaatttt ttttccaggc atgatatttc
      961 ctatcgggaa gtaacggcag ctaggtaatt gaaataactt ttcttgcgat ctgtgttgat
     1021 ggcccagtta ataggtatca attggtaacc gtttcggtaa tcgccaaagg ccgcgtttcc
     1081 gcctgctccg tcaccaggat caaagtatcc ccagcctgca gaagatctaa ttgcgcaata
     1141 gaagttattt aattcatgat tgaagtcaaa atgatcgtct tcattaaata atatgggcat
     1201 ttcgtgccac tctgttagtg ctctggtttc ttctatcatt tccgaaattc ttttgggatc
     1261 aacaacaccg ttcccatgca taaggacaaa atcagatgac ttaaccacat tgttctcagg
     1321 gatagtatta ccgccgtagc tggtgccaac taaaagtctt tttgactcag atttgatgga
     1381 tttaactaat tctatgagtt catgaactct atgtggttgg agtatgtcgt gttcatattt
     1441 tgggacactg cattcgttat taatttctat cacaacgtta tcatatccac tagttaggag
     1501 ccactcacag gtatttcgaa ccgctgatat cactgacgct tcgtctctga ctctttcatc
     1561 ttgtccgaaa taaaataaac ctagtataag caccatcgat aatctatcag tttcttcaat
     1621 tattttgcgg agcctacgga tgtaatcaga ttttaagctg ccgtcaggat tgaagccgga
     1681 actattccac ggttgagatt caggagatgg taggcctttc catatttctg aatcttttcc
     1741 ggatatccct ctctgtttca taaattctct gaattgattc agccgataat atccagaagg
     1801 tgctcctcct tgtaaattaa ctgtgatggc ggtcaatcca tattttttgt aatcaggtaa
     1861 catacggata agttcattgg tgttcctgtc tgggtcccat tcggttgtgt cagggtattt
     1921 ccaaatatgg cgtgtatagg gattgtcatc gtcgaagatt gcgttcacca tccgcgcatt
     1981 tagcaataac ccttctatgt taataccttg gtaggtaata cctttatatg tgggtatgcc
     2041 atcgatcaac catttttcac catggataga cacccgagtc atatcttgct taatccttgc
     2101 tgttctcttc tttttgcttt gctaccctgc gggacccgcc catatctgag ccaggcaatg
     2161 gcacagttcc aataatttcg tcggttttaa atttatttat agtttctctg ctgtacccaa
     2221 gtaattcttc taatatagat tcgttatttt gaccaagaca aggtgctggg ttgtgggtta
     2281 cccgtccatg gtcgctttca aacttaaata ttggcccagt catggggtag agaccagcag
     2341 ctggatgatc aactatttga aaaaaatccc tttcgtttaa atgcgggtct gcataggaat
     2401 ctgcaccgca gtctataaca accccagatg ttatacctgc ggcttgcaaa agggtcagcg
     2461 catcatatct atcccgagtc gaagcccatt tatttatcac attatcaagg tggtcatggt
     2521 ttttaagccg accttccaac gaatctaagc ctgtttctcc tagggagtca tccccaacaa
     2581 ttgttttaag ttcacaccac tctttctctg ttttgatact tatcgctatc caattatcat
     2641 ctccagaaca tttatatacc ccctgtgggg cgaaatctgg gtgacgatta cctcttcgtt
     2701 ttctaattga cgatgtcatt tggttctcaa gatatatttc accgagatgg gtcataaatg
     2761 actcggcctg tgatagatct ataaattggc ctttgcctgt ctttgctcta tataccaaag
     2821 ctgaggtgag ggccattaca ccgtttgtag ctgccacagc atctgggtga tgaaccagtg
     2881 aattcgaaga gtggttcaga tcaggatacc ctctcaaaaa agagtgtcct gtaattgcgt
     2941 ctattgccat tcccatactg cggtagttgg cgtaaggccc gtcatttcca taacccggca
     3001 ttagcatgta gatgatatcg ggttttatct ttctgacctg ttcgtaactg agtccaaagt
     3061 tttccatcac tgtacgtgca taattacaga aaacaacatc actttgtgaa actagatctt
     3121 tgaaagaagt taatccttgt ggggttttca agtccaaagt caggcttttt ttgttccgat
     3181 ttatggcgtt gaaatttgac tgtcggttcc atggttcttt cccagggtca tcatctggat
     3241 accctcccgc gttgggggga ggggttattg
//
LOCUS       DASCNS010000100         2487 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000846375, whole genome shotgun sequence.
ACCESSION   DASCNS010000100 DASCNS010000000
VERSION     DASCNS010000100.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000846375"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="QF838_03635"
     CDS             complement(<1..188)
                     /locus_tag="QF838_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59816.1"
                     /translation="MNYADIILISPEISMALLASVIIIVDLFSSNKKILAPLSLIGLL
                     APLCFCIMVRDNPSLGFKG"
     gene            complement(188..1666)
                     /locus_tag="QF838_03640"
     CDS             complement(188..1666)
                     /locus_tag="QF838_03640"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HJO59817.1"
                     /translation="MEIPGILSIAIFLPFISAVIVAVFLTGKKARGFAVIASTLELVV
                     TGYIFIAHRQNGYDMTEKITTWFPESFPVQYFLSVDGISTPLILLTGLLGIVAIFASW
                     NITERTKEHFMWLLLLQGAVIGVFTSMDFLLFFLFWELELIPMFFLISIWGTGRKDYS
                     AMKFLIFTILGSAFMLIGILVIFAGTGTFDMTKISEVLPSSNLIMPVGILFLFMSIGF
                     AVKLPVFPLHTWLPDAHTDAPTAASVMLAGVLLKMGGYGLLRVSAGMFPDQLEKYAWV
                     LALLGVINIIYGAAITIRQTDLKRLIAYSSVSHMGFVLLGLSAVGGGITSLAMTGAAL
                     QMFTHGTITGLLFLTIGFIYERTHTRHIPDLGGLARRMPIFTVSLVVAGLASLGLPGT
                     SGFVSEVTIFLGAFPAFKLATIIAAFGVVLTAAYILWMIQRSMFGPEKERFAQIGDAT
                     PVQVIPMGLLIVSIIGVGIYPAFVSDVFAEGITQMLNMTGAN"
     gene            complement(1679..>2487)
                     /locus_tag="QF838_03645"
     CDS             complement(1679..>2487)
                     /locus_tag="QF838_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59818.1"
                     /translation="ITFLIGSLSLAGIFPLAGFWSKDEILLNALDKGNVASIIGFSLA
                     LIAVFMTAFYMFRALLLTFEGEFRGGSDKDPEAQAHGPVDLTESSFPMVLPLVILAIP
                     AVFIGFLVNPIKNFGGIPAHWFTHKLEHSLHLPPPPAFDPLLAIISSLIALLGILLAW
                     AMYKRNPYLSPATMGRVSGPIYTLFLNKYYVDEAYEKFITGRIYYRGIALIFDWIDRN
                     IVDRTVNIIGWLGANFGSLIRELQTGQTQMYATVTSVGIIIIAAVYIFGM"
BASE COUNT          772 a          532 c          510 g          673 t
ORIGIN      
        1 cccttgaatc caaggctcgg attatctcgc accataatac aaaaacatag cggggctaat
       61 aagccaatta gagacagagg agccaaaatc tttttgttag atgaaaacaa atccacaatt
      121 attattacac tagccaataa ggccatgctg atttcgggag atatcaatat tatgtcggcg
      181 taattcatta attagccccc gtcatattta gcatttgtgt tatgccttcg gcgaaaacat
      241 cagatacaaa tgctggatat ataccaacac caattattga aacaataagt agtcccatag
      301 gaatgacttg cactggtgtg gcatcaccaa tttgcgcaaa ccgttctttt tcaggtccaa
      361 acatggatct ttgaatcatc cataatatgt atgcagccgt taggacaact ccaaaggcag
      421 cgattattgt cgctaattta aaggctggaa aagctcctag aaagatagtt acctctgata
      481 caaacccgct tgttcctggt agtcccaaag atgctaatcc tgcaactaca agcgatactg
      541 tgaagatcgg cattctacgg gccaaacctc ctaggtctgg tatatgtcga gtgtgagtcc
      601 tttcatagat aaatcctatt gttaaaaata aaaggcccgt gatagttcca tgggtaaaca
      661 tttgtaaggc ggcacctgtc attgcgagag aggtgatgcc gccacccaca gctgacagtc
      721 ctaataaaac gaaacccatg tggctgacac tactatatgc aatgagcctt ttgagatctg
      781 tttgtcggat tgttatcgct gcaccataga taatatttat tacccctaat agggccaata
      841 cccaagcgta tttttcaagc tggtcgggga acatacccgc cgatactcgt aaaagcccat
      901 atccacccat ttttaaaagt actcctgcca acattacgct tgcagcagtc ggggcgtcgg
      961 tatgagcatc tggtagccag gtgtgtaaag ggaatactgg tagctttacc gcaaagccta
     1021 tgctcatgaa taagaagagt attccaacag gcattattaa gttcgaggat gggagcactt
     1081 cagatatctt agtcatatcg aaggtgccag tacctgcgaa tataaccaga atcccaatta
     1141 gcatgaaggc gcttccaaga atagtaaaaa ttaaaaactt catagcggag tagtcttttc
     1201 ttccggtacc ccaaatggat attaagaaga acataggaat caactctagc tcccaaaaaa
     1261 ggaaaaaaag taagaaatcc attgaggtaa atacccctat taccgcccct tgtagcaaga
     1321 gaagccacat gaaatgttct ttagttcttt cggttatgtt ccatgatgcg aagattgcga
     1381 cgattcctaa cagccctgta agcaaaatga gtggtgtgct tattccatcg acagatagaa
     1441 aatattgaac tggaaatgat tcagggaacc acgtagttat tttttcagtc atatcgtacc
     1501 cattttgtct atgggcaatg aaaatgtaac cggtgaccac tagttcaagt gttgaagcta
     1561 taaccgcgaa tccacgagcc tttttcccag tcaaaaaaac agccacaatc accgctgata
     1621 taaacggcaa aaagatggct atgcttagta ttccaggtat ttccacagat tctcctaatt
     1681 acattccaaa tatatataca gcggctatga tgattattcc aactgatgtg acagttgcgt
     1741 acatttgggt ttggccagtt tgtaattctc ttatcagact accgaaattg gcacctagcc
     1801 acccaatgat attcacggta cggtctacga tatttcgatc tatccaatcg aatatcagag
     1861 ctatgcccct gtaatagatt ctgccagtta tgaatttttc atatgcttca tcgacataat
     1921 atttgtttag aaatagggta tatataggtc cggaaactct gcccatcgtt gctggggaca
     1981 aataaggatt ccgtttatac attgcccatg ctaggagtat tcccaatagg gcaattaacg
     2041 aggaaataat ggccagtaat gggtcaaaag ctgggggagg gggcaaatgc aggctgtgct
     2101 ccaatttatg tgtaaaccag tgggctggga tgcctccaaa gttttttata ggattcacta
     2161 gaaaccctat aaacacagct ggaattgcga gtatcaccaa gggtaaaacc atagggaatg
     2221 atgattctgt aagatcgacc ggcccatggg cttgggcttc agggtcctta tccgatcctc
     2281 ctcgaaattc cccctcaaat gttagtaaca gtgctctgaa catataaaag gctgtcataa
     2341 atacggcaat gagtgctaag gagaatccga ttatactggc aacattccct ttatctagcg
     2401 cattaaggag tatttcatcc ttactccaaa atcctgccag tgggaatata ccggcaaggc
     2461 ttagacttcc aatgagaaag gtaatat
//
LOCUS       DASCNS010000101         6183 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000847668, whole genome shotgun sequence.
ACCESSION   DASCNS010000101 DASCNS010000000
VERSION     DASCNS010000101.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000847668"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="QF838_03650"
     CDS             complement(<1..753)
                     /locus_tag="QF838_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012725.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJO59819.1"
                     /translation="MFSSISTILDVIIFVLSGFIVSSTVAAIVFVVWTFGKMTERTTT
                     KELLDKINSSSKKTNTPSPLNTTDKNGELIKAAITIVESYGLSVSGSQNSRAMNITSL
                     EDNLNQATEPNNSNRILPIPENSQEKMLLQTGHVHMKHGDFQSAIKDYTNAIIINPTF
                     AGAYNARGMANRKMKDFHSALQDYNTALSLHNNFAATYNNRGVIYMEIRQIEAALTDF
                     DKARHIEPNNSYGYCNRAVAYNYMRDFNKSISE"
     gene            872..1723
                     /locus_tag="QF838_03655"
     CDS             872..1723
                     /locus_tag="QF838_03655"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HJO59820.1"
                     /translation="MKRVVGYYSIELGAGSDVGSVREQNEDAYHTLLGTGSEDELFDA
                     LLIVADGMGGHAAGEVASEMAVTNLPKHLVKALSSGQNNEDIENVLSECVVKTNMDIF
                     EKSKEEDTRGMGTTLTAGVVVQDRFFYAHVGDSRGYLLRENAFEQFTTDHSWVAEQVA
                     LGILTEEQAENHPRRNIITRAMGLDSNVEVDSGFIELELSDQILLCSDGLHGLVTAGD
                     MMEICVEEEPEDAFQSLIDMANSRGGHDNITVVVAKIGEGDNYDPRARGDGIKSVLGN
                     IKNLFSD"
     gene            complement(1733..2389)
                     /locus_tag="QF838_03660"
     CDS             complement(1733..2389)
                     /locus_tag="QF838_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HJO59821.1"
                     /translation="MTQDSNINVIVADDHAVVREGITRILATSDRIKLIGEAESGDEA
                     WLLIKDMVPDVALLDIRMPAMTGIEIIKSINAAGLKTKSLILTNYDNPDYILEAISEG
                     AHGYILKAIDPTSLIKSVVAVYEGEVVLDPEVASLVAKTWRERNSRTSSIMGISSLTI
                     REKETLKLACDGLRNREISDEMGISVRTVEGHFNRIFSKLGVSSRTEAVLQAVSEPIQ
                     "
     gene            2681..>6183
                     /locus_tag="QF838_03665"
     CDS             2681..>6183
                     /locus_tag="QF838_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HJO59822.1"
                     /translation="MDLKEIGGYQVIEMVGEGGQGAVYRAQDPSTGQIVAIKVLSRTA
                     SDGEFLDRFQREASIMATLRHPNVVEVYDHGEENGQHYIVTEFVTENLERILERGGTL
                     PLQRAITVVQQIASALQVSHNAGITHRDIKPANVLLNDAGDVKLTDFGIASAETLDSM
                     TSENTTVGTPLYMSPEQIQGSDAIDGRSDLYSLGCLLYEVLTGATPFPGKSTFEIFNG
                     HINDSHVPIAEHMDEFPEQLEDLLIKSLTKDRDQRYQTADEMINDLQVITTILAEGPQ
                     DVARTRVMPKVLATQVIDKPNTTGSSTTGGGGMPKWLMPAGAIVALLVIIGGGAFFFM
                     GQDSGAVGLATGIAADISGENWAPDIIANPEGVQQAASELTTAYGQDPVGAVAGINRL
                     DDLDPMFAASVILGIHAQNQSQAAQILFSIGDADELRLEGHLARITEMDPVQAGDILI
                     SLINEDVAEVASLMEDVVEGDNGQILQTMFVEAAMNDDLAIAQLVNQAAMTPGAEQST
                     ANALISIANADIEIAAAILAGTNTGSSSIWQAYIAIEPTGAGATFVKAYKNGFYDEVT
                     GKIINILPDIYVSGLLLSHMAREDSAETARVYASLMDNQDAGPVSVSPAIVEAARFDY
                     VAVEKMLTEDASFIGHFSQDAHPQIWTTMELPMAGDGEEGKGIWTKPSLDSSSLINFS
                     SIVVKSLNASDIESGTVGIFDVTPPVSDTRQDRNVLTHVQIDTAGFISNGVTTILVFD
                     VPKSHFVVNETGFAEGHPWSVELSRFNDTSQTWNPVFANTIGEDEENITFAAPVAGLS
                     QYGTSFWSVSTSSTSIAGSQLSIDQIDTSPAGNGQVHVQATVTNRSPQAVSRDISLII
                     DGIPEYSTTVEVDSQSVVIVETDIFMQPGMRNLSLGNHNTSYENLLVAGSTGDSGSTG
                     DSGADGSLSGSDLASMGADCPSESEVSDNEVHYGTEIESTIDCAGDIDQWVFEGSAGD
                     LVTIQMKAGPGSDLDAAIDLVSPNDKLVKSDDDSNDSSTPPVNGIDSEITSYELRFDG
                     MYSILSKGGGTGGYNLVVMTSDNSIPDAPAQTVLSAADDTGDSNSDGVTKNEYNLSFD
                     IERISNFGTKPHIRLWRGNDIEIGACEADEYDGTCTVVVSELSEGEHYIYATAISEMG
                     TSSSRSDSVLITIDTEIETPS"
BASE COUNT         1723 a         1219 c         1494 g         1747 t
ORIGIN      
        1 ctcactgata cttttgttga agtcacgcat atagttataa gccactgccc tattgcaata
       61 cccatatgaa ttgttaggtt ctatatgccg agctttgtcg aaatcggtta aggccgcttc
      121 tatttgtctg atttccatat aaataacacc ccgattgttg taggtggctg cgaagttatt
      181 atgaagactt agagcagtgt tgtaatcctg tagggccgag tgaaaatcct tcatctttct
      241 atttgccatg cctctggcat tataggcacc ggcaaatgtt ggattgatga taatagcatt
      301 ggtgtagtct ttaatcgctg actggaaatc gccatgcttc atgtgaacat gacccgtttg
      361 caataacatt ttttcctggc tgttttctgg tatgggtaaa attcgattcg aattattggg
      421 ttcggtggct tgatttaaat tatcttccag tgaagtgata ttcatggcac gggaattttg
      481 gctgccagag actgataatc cataactttc aacgattgtt attgcggctt ttattaattc
      541 accgttctta tcagttgtat ttaagggcga aggggtattg gttttttttg aagaactgtt
      601 tatcttatct aaaagttctt ttgttgttgt gcgttcggtc attttgccga acgtccaaac
      661 cacaaatacg attgctgcaa ccgtgctgga tactatgaag ccagataaaa cgaatataat
      721 tacatctaat atggtcgaga tactagaaaa cattaaccaa atgggcctat atcaataatc
      781 gttgaatgaa atcccgcttg ttaaagtata acaatctacg tgcctatatg gttcgccttt
      841 tacaaacttg tatgtcttat agagaaaaaa tatgaaaaga gtcgtcggat attattccat
      901 agaattgggg gcaggttctg acgtggggtc tgtcagagag cagaatgagg atgcatatca
      961 tacgttgctg ggtaccgggt ctgaggacga gctttttgac gcccttttga ttgtagctga
     1021 cggaatggga ggccatgctg ccggagaggt ggccagtgaa atggctgtga ctaatctgcc
     1081 caaacattta gtcaaggctt tatcttctgg tcaaaataat gaagatattg aaaacgtact
     1141 gagtgaatgt gttgtaaaaa ctaatatgga catcttcgag aaatcgaaag aagaagatac
     1201 gagaggtatg ggaactactc ttactgcagg agtagtggtg caagacagat ttttttatgc
     1261 ccacgtgggt gatagcagag ggtatctgtt gagagaaaac gcttttgagc aattcacaac
     1321 agatcatagc tgggtggcag aacaagttgc cttgggtatt cttaccgaag agcaggcaga
     1381 aaatcaccct cgaagaaata taatcacaag agcaatgggg ttagattcaa atgttgaagt
     1441 tgattccggg ttcatagagc ttgaattatc ggaccagata ttgttgtgtt cagatggtct
     1501 tcatggatta gtgacagccg gggatatgat ggaaatttgt gtcgaggaag agcccgagga
     1561 tgcttttcaa tcactcattg atatggccaa ttcaaggggg gggcatgaca acatcacggt
     1621 ggtggttgct aagataggag aaggtgataa ttacgatcca cgtgcaaggg gcgacggaat
     1681 caagtcagtg cttggaaata tcaagaattt attctcagac tagcctggaa ggttattgga
     1741 tcggttctga tactgcttgg agaacagctt ctgtccgaga agagacaccc aattttgaaa
     1801 atattctgtt gaagtgacct tcgactgttc gaacacttat ccccatttcg tccgatattt
     1861 ccctgtttct gagaccgtcg caggctaatt taagggtctc tttttctctt attgtgaggg
     1921 acgaaatccc cattatggaa ctagttctag agtttcgctc tctccatgtt ttagctacta
     1981 aacttgctac ttcggggtca agtactactt caccttcata gacagcgacc actgatttta
     2041 taagagaggt cggatcaata gcctttagta tgtagccatg ggcaccttct gagatcgcct
     2101 ccaggatgta atctgggttg tcgtaattgg tgagtataag agattttgtt ttgagtcccg
     2161 ccgcattaat tgatttaatg atttctatac ctgtcatagc aggcattcgt atatcaagta
     2221 aagcgacatc ggggaccata tctttgatta atagccatgc ctcatcccca ctttcggctt
     2281 cccctattag tttaattcgg tccgaagttg ccaaaatacg ggtgattcct tcacgaacta
     2341 cggcatgatc gtcagctacg attacattta tatttgagtc ttgcgtcatc ggcctacccc
     2401 tctccggacc ctatattgta gtttgctata gaattctcta catagagata tagaaaattt
     2461 acttgtctta agcacagtat atataattgt atagcttgca agtttgatta ataattaggt
     2521 gtaattactt atatatatta ggtgcccata cccgtgaaat atagttgttt atatggtgta
     2581 ctttacatca tacttactag cgatatcgag gtgagcgtca atatatcctt atatgatata
     2641 caaataagca aagtaaatgt aagggcttgg ctataaaaaa atggacttaa aagaaatagg
     2701 cggatatcaa gtcattgaaa tggtcggtga aggtgggcaa ggcgccgttt atcgtgccca
     2761 agacccatca accgggcaaa tagttgctat caaagttctg tctcgcacag catcggacgg
     2821 ggaatttctg gataggtttc aacgtgaagc ctcaatcatg gcgacactta gacaccctaa
     2881 cgttgttgaa gtttatgacc atggtgaaga gaatggtcag cattacatcg taactgaatt
     2941 tgttactgaa aatcttgaaa gaatattaga aagaggtgga actttacctc tccagagagc
     3001 tatcacagtt gtccagcaaa ttgcctcggc acttcaggtt tctcataacg cagggataac
     3061 ccaccgagac ataaagcctg cgaacgtatt gctgaacgat gctggggatg taaagctaac
     3121 tgatttcggt atagcctcgg cagaaacatt ggattcgatg accagcgaga acaccactgt
     3181 tggtacgccg ctctatatgt ctccagaaca aatacaaggc tcggacgcta tagacggtag
     3241 gtcagacttg tactcactgg gatgcctatt gtatgaagtc ttaactggag caactccttt
     3301 tccgggtaaa agtacctttg agatttttaa tgggcatata aacgactctc atgttccaat
     3361 agccgaacat atggatgaat ttccagagca gctggaagat ttgttgatca aatcgttgac
     3421 caaagataga gatcagcgct accagacagc tgatgaaatg atcaatgatt tacaagtaat
     3481 caccaccata ttggcggagg gacctcaaga cgtggcacgt accagagtta tgcccaaggt
     3541 tctggccaca caggtgattg ataagccaaa cacaaccggc agtagtacta ccgggggtgg
     3601 aggtatgcct aaatggttga tgcccgcggg agccatagtg gccttgctgg ttataattgg
     3661 tggtggagct ttcttcttta tgggtcaaga ttctggcgct gttggattgg cgacaggtat
     3721 cgcagctgac atatcgggag aaaattgggc cccagacatc attgctaacc ctgaaggtgt
     3781 gcaacaggcc gcatctgaat tgacgacagc ctacggccaa gaccctgtag gtgcggttgc
     3841 aggaataaat cgattggatg atttggaccc catgtttgca gcgtctgtga tactgggcat
     3901 tcacgctcaa aatcaatcgc aagcggcgca aattcttttc tcaataggag atgcagatga
     3961 attgcgactt gaaggacatt tggccaggat caccgaaatg gatcccgtcc aggccggtga
     4021 cattctaatt tctctgataa acgaagatgt ggctgaagta gccagtctga tggaagacgt
     4081 agttgagggg gacaacggac aaattcttca aaccatgttt gtggaagcgg ccatgaatga
     4141 tgatttggca atagcacaat tggttaatca agccgcaatg acgccgggtg ctgagcaatc
     4201 caccgcgaat gcgcttatct ccatcgcaaa tgctgatatc gaaattgctg ctgctattct
     4261 tgccggtaca aacactggtt cctcttcgat atggcaggcc tacatcgcca ttgaaccgac
     4321 aggtgcaggt gctacgtttg ttaaggccta taagaatggt ttttatgacg aggtaacagg
     4381 aaagataatc aacatcctcc cagacattta tgtttctggt ttgctgcttt ctcacatggc
     4441 gagagaggat tcagcggaaa cagcaagagt ttatgcaagt cttatggata accaagatgc
     4501 tggccctgtg tcggtgagcc cagccattgt tgaggctgcg aggtttgatt acgtcgcggt
     4561 ggagaagatg ttgacggaag atgcaagctt cataggacat ttttctcaag acgcgcatcc
     4621 acagatatgg acgacaatgg agctaccaat ggctggcgac ggtgaggagg gaaaaggaat
     4681 ttggaccaaa ccttcacttg actcttcatc actgataaat ttcagttcca tagttgttaa
     4741 atctttgaat gcatctgaca tagagtccgg taccgttggc atctttgatg ttactccacc
     4801 agtatcagat acccgccaag accgtaacgt tttgactcat gtgcaaatag ataccgccgg
     4861 atttattagt aacggggtaa ccactattct ggtgtttgat gtacctaagt cacattttgt
     4921 agttaacgaa actggctttg cggaaggcca tccctggtct gtagaactga gcaggtttaa
     4981 tgatacttcg cagacatgga atcctgtttt tgcaaacact atcggtgaag atgaagagaa
     5041 tataaccttc gcggccccag tggcaggcct ttcccaatat gggacttcgt tttggtcagt
     5101 atcgaccagt agcacgagta tagcggggtc acagttatca atcgatcaaa ttgatacctc
     5161 tccggctggg aatggtcagg ttcacgttca ggctacagtg actaatagaa gcccccaagc
     5221 cgtaagtcgc gatatttctc tcatcattga tggaattccg gaatattcaa ctacggttga
     5281 ggtggattct caaagtgttg taatcgttga aaccgatatt ttcatgcagc cgggaatgag
     5341 gaacctttcc ttaggcaacc acaacaccag ttatgaaaac cttcttgttg cagggagtac
     5401 cggtgactct ggtagtaccg gtgactctgg tgcagatggc tcgctttctg gaagcgacct
     5461 ggcttctatg ggtgcagatt gtccatcaga atcggaagtt tcagataatg aggttcatta
     5521 cggtactgaa attgaatcca cgatcgactg tgctggagat attgaccaat gggtatttga
     5581 gggaagtgca ggagatttgg ttactatcca aatgaaagca ggtccaggat ctgatttgga
     5641 tgccgccata gatctcgtta gccctaatga caagctcgta aagtctgatg atgattctaa
     5701 cgactcatcg acccctccag ttaatggtat tgattcagag ataacgtctt acgagttaag
     5761 gttcgacgga atgtattcaa ttctcagcaa aggcggtggc acaggcggct acaacttggt
     5821 agtcatgact tctgataact caatacccga cgcgcctgca cagacagtgc tcagtgctgc
     5881 agatgatacc ggagacagta attcagacgg tgtcaccaag aacgaataca atctatcgtt
     5941 tgatatagaa aggatttcga attttggcac caaacctcat ataaggttgt ggcgtggaaa
     6001 tgacattgag attggagcct gtgaggctga tgagtatgac gggacctgta cggtcgtggt
     6061 cagtgaactt tcagaaggag aacattacat atatgcaaca gctattagtg agatggggac
     6121 ttcgagttca agatcagatt ctgttcttat aacgattgat acagaaatag aaacgccgtc
     6181 aga
//
LOCUS       DASCNS010000102         2182 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000850639, whole genome shotgun sequence.
ACCESSION   DASCNS010000102 DASCNS010000000
VERSION     DASCNS010000102.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000850639"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="QF838_03670"
     CDS             complement(<1..1436)
                     /locus_tag="QF838_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013871.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJO59823.1"
                     /translation="MKPPTAGYYRLPTQVIHIGSALSQIPVKSYLVGGVLRDSILGTT
                     NPDVDIVVVGSALDVSKKVATIMNGKSFPLDTSRDIYRVLAKVSETEIQIDIASAQNG
                     ISSDIEKRDFTINSLALDVNDVNCNDGVPRFEISKIIDEHRGIEDILNGSLRMTSSQV
                     FKKDPLRILRGARLTAQYNLDIDKYTETQIKESSSLISRVSSERIRDEFLKLLSLQQS
                     TRNLTKMDELGILTNIIPELEASRETSQPPSHHWKVLEHMVQTVGQAENIVTGKQINA
                     GPYPKFTLKYIPSTDAHLTYFEQNYSDSQSRLTFLKLACLLHDVGKPKTKTVDPDGKT
                     RFLGHDKVGGEIARSILKRMKFSNSGIELVTKQISNHLRPSQISNQGQDPTPKAIRKY
                     YNDTSGASIDILYLNLADYISAKGPKLTQTEWIDHCRKINFIAKSESSYKRDKNQAKL
                     VSGHDIMVGLCLNPGPFIGTLIKDVEEAR"
     gene            complement(1447..1986)
                     /gene="def"
                     /locus_tag="QF838_03675"
     CDS             complement(1447..1986)
                     /gene="def"
                     /locus_tag="QF838_03675"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HJO59824.1"
                     /translation="MPILPILEIPHPILRKKAKKVRSISKKELTLAYSMVDTMREAGG
                     VGLAANQIGELWRIIVLQMPDEEESRIYFNPQIIKTYGSREVEEGCLSLPGYRGIVNR
                     SMWIKFQGLDHTDQTVKFEAENLLSQALEHEIDHLNGILYTDHLAEHQQLYTVTHEGE
                     EFDEQSVETAESPASIKID"
BASE COUNT          571 a          439 c          415 g          757 t
ORIGIN      
        1 cgagcttctt cgacatcctt tattaaagtg ccgataaaag gcccagggtt aagacacaac
       61 ccaaccatta tatcgtgtcc tgatacaagc ttagcctgat ttttatccct tttataggaa
      121 gattcacttt tcgctatgaa atttattttc ctgcaatgat caatccattc agtctgagtc
      181 agcttaggtc cctttgcgga tatgtaatca gctaaattta aataaagtat gtctatggag
      241 gctcctgagg tgtcattgta gtatttacgt atggctttag gtgttgggtc ttgaccttga
      301 ttggatatct ggctaggtct taaatggttt gatatttgct tggttactag ttcgattccg
      361 gaattactaa atttcattcg ttttagtatt gaacgggcta tttctcctcc tactttgtcg
      421 tgccccagaa atcgtgtttt tccatcagga tctacagtct tcgtttttgg ttttcccacg
      481 tcatgcaaaa gacaggcaag tttcaaaaaa gtgagtctac tttggctgtc ggaataattt
      541 tgttcaaaat aagttaggtg ggcatccgtg cttggaatgt atttcagggt gaattttggg
      601 taggggccag cattgatttg tttgccagtc actatattct ccgcttgccc cactgtttgt
      661 accatatgtt ctagaacttt ccagtgatga ctgggaggtt gtgaggtctc cctggaggct
      721 tctagttctg gaatgatatt ggttaaaatg cctaactcat ccatttttgt taaatttcta
      781 gtagattgct gcaaagaaag aagttttaaa aattcgtctc ttatacgttc cgaggatacc
      841 ctggaaataa gagagctact ttctttaatt tgagtttctg tgtacttatc gatgtctaaa
      901 ttatattgag ctgtgagcct ggcaccccgt aaaatcctta agggatcctt tttgaatact
      961 tgactgcttg tcattcttaa tgaaccattg aggatatcct caatacccct atgttcatcg
     1021 atgatttttg atatttcaaa tcgtggtaca ccatcattac agttaacatc atttacatct
     1081 aatgctaggg aattaatagt gaaatcacgc ttttcaatgt cagaggatat accattttga
     1141 gccgaggcta tatcgatttg tatttcggtt tctgaaactt ttgcaagaac cctgtaaata
     1201 tctctggatg tatctaaggg aaaacttttg ccattcataa tagtggctac ttttttggag
     1261 acatctaaag cgcttccaac cacaactata tctacatctg gattggttgt accaagaatt
     1321 gagtccctta atacacctcc aaccaaataa gatttcacag ggatttggct tagtgctgaa
     1381 ccaatgtgga tgacttgagt aggcaaacga taataaccgg ctgttggagg tttcatcgta
     1441 aatgacctaa tctattttga tagaagctgg gctttccgcg gtctcgacac tttgttcatc
     1501 aaactcttcg ccctcgtggg tgacggtata cagctgttga tgttctgcta ggtggtctgt
     1561 gtataaaatg ccattgaggt gatctatctc atgttcaaga gcttgtgaca gtagattttc
     1621 tgcttcaaac ttaactgttt gatccgtgtg gtccaaaccc tgaaatttta tccacatgga
     1681 acgattgaca atgcctctat aaccaggaag actcaggcat ccttcttcaa cttcccttga
     1741 accgtatgtt tttattatct gtggattgaa atatattctt gattcttctt catcaggcat
     1801 ctgcaatact atgattctcc agagttctcc aatctgattt gccgcaagcc ctacaccgcc
     1861 agcttccctc atggtgtcaa ccatgctata ggcaagggta agttcttttt ttgatataga
     1921 ccttaccttc tttgctttct ttcttagtat cgggtgggga atctctaaaa tcggtaatat
     1981 tggcaagaat tctcctccaa ccttctgact caattcatag gtcgtgtttg atcagttaga
     2041 acccatataa taaatcaaaa cttatttcct gtcccaatat ccttcctcat attcatctgg
     2101 tcttaaatga tcgtcagaat cattggtgtt aactttatat ctcaaccatc ctattcctga
     2161 aaacaaaaga cctattaaaa ag
//
LOCUS       DASCNS010000103         9791 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000869518, whole genome shotgun sequence.
ACCESSION   DASCNS010000103 DASCNS010000000
VERSION     DASCNS010000103.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000869518"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="QF838_03680"
     CDS             complement(<1..1360)
                     /locus_tag="QF838_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015882.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HJO59825.1"
                     /translation="MTVRRQPGAAHSITIRTSYPNTVGMLGQITTNISAAGGDIGAID
                     IVSAGRNGMVRDITINARDDSHAEILKKAVSSVEGVTIVNVSDQVFLRHLGGKIEMQS
                     KAPIKTRNDMSLIYTPGVARVSKAIHEQPESVWNLTSKGNTVAIVTDGSAVLGLGDIG
                     PAAALPVMEGKAQLLKELVGVDAWPIVLDTHDTNKIVDTIKLLSPGFGAINLEDISAP
                     RCFEIEQRLHKELAIPVFHDDQHGTAIVVLAALYNAVKVVNKNLPELKVVLVGVGAAG
                     VATTKLLIKAGIKNIIGVDRLGILDRQKDYGNNKVWKEYSKITNPHNISGQITEAVTG
                     ADVFIGLSGPEVLQIEDVSLMNTNPIIFAMANPDPEIWPEEVEGLVEVMATGRSDYPN
                     QVNNALCFPGMFRGILDARAQKVTDEMKMAAAFAISKGVSESHINNEYIMPSIFDTDV
                     ADQ"
     gene            1838..2923
                     /locus_tag="QF838_03685"
     CDS             1838..2923
                     /locus_tag="QF838_03685"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HJO59826.1"
                     /translation="MHKSKAVIARNRGERVEEVEILVPEPGPNEALIKIKACGVCHTD
                     LHYREGAINNEFPFLLGHEAAGYVEEIGEGVTNVTVGDFVIIAWRSPCGRCRSCLKGK
                     PWYCFDSRNAVQNMSLEDGSTLSPALGIGAFSELTLVDAGQAIKVDAAVNSNTASLLG
                     CGVMAGLGAAINTGSIGMGDSVAVFGCGGVGNAAIVGSKLAGAQKIIAVDIDDQKLEW
                     AMEFGATHTVNSRSTDPVDFIRSVTDGNGTNVTIEAVGNPKTYEQAFYARDHAGVLVL
                     VGVPDPTMRIELPLIEVFGRGGSLKSSWYGDCLPSRDFPKFIDLHLDGRINLDKFVTE
                     TISMSEVETAFEKMEAGKVLRSVVVFE"
     gene            2981..3274
                     /locus_tag="QF838_03690"
     CDS             2981..3274
                     /locus_tag="QF838_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HJO59827.1"
                     /translation="MVITVRSRKMVSGSVNSLMKTIDDGFMPIISEEPGYIAYYVVDS
                     GDGLVTAVSIFEEEETSVKSNNLAQEWISKHLGDLVPGKAQVISGPVMVPSQS"
     gene            3291..4145
                     /locus_tag="QF838_03695"
     CDS             3291..4145
                     /locus_tag="QF838_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HJO59828.1"
                     /translation="MPRLKYPLAVPVKAGLKTINLFELRAETSLRDNVNDFREYADVL
                     YSAHAPTTIGEERLNIAATDSDFRNECILVFKDYIDRCAMFPNIGQINMHFGLKNWVS
                     ETQPRGQKGDYETHIESIRTLSDYARTSGIEIVLENLNCYWALNNISDDTDFAQVNWD
                     NSNEAFGMNPEEWIEMCLDVDRDNVQLCLDTSHVSTYGHRFPEEERAGKIEAFLNRPD
                     LITHVHWSDNYLYDVRGRNDSHLSVGKGSIPTDFHRRVKALQATILLEHFYTKEELHE
                     ELEYIERL"
     gene            complement(4230..4315)
                     /locus_tag="QF838_03700"
     tRNA            complement(4230..4315)
                     /locus_tag="QF838_03700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4280..4282),aa:Leu,seq:cag)
     gene            complement(4322..4398)
                     /locus_tag="QF838_03705"
     tRNA            complement(4322..4398)
                     /locus_tag="QF838_03705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4362..4364),aa:Val,seq:cac)
     gene            complement(4444..4516)
                     /locus_tag="QF838_03710"
     tRNA            complement(4444..4516)
                     /locus_tag="QF838_03710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4481..4483),aa:Met,seq:cat)
     gene            4572..4922
                     /locus_tag="QF838_03715"
     CDS             4572..4922
                     /locus_tag="QF838_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59829.1"
                     /translation="MDSTTKPNIEADVILNETNAKLHNLLIGLASSVANFPGFLGMET
                     VQAIEVEPIVGFPDRGCIVVTPEGFLKELILSVLPGASTLGGYEQSEQLKDLDLPPEE
                     ETVYLYRAIKLLSK"
     gene            4959..5801
                     /locus_tag="QF838_03720"
     CDS             4959..5801
                     /locus_tag="QF838_03720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HJO59830.1"
                     /translation="MGNIGITTFPTDYAIDIAVLAAKAEEAGFDSLWVAEHPVLPVGA
                     ETPWPGPGGVIPKKYADVADPFVALGRASAVTDKLLLGTGICLVPERNPLILAKEVAT
                     LDMYAKGRFLFGIGAGWLKEETDIMGGDFAHRWTQTRESVMAMKELWTTVESEYHGKY
                     YDFPPVYSFPMPVQRPHPPIYLGGHAKNVFKRVVSWGDAWMPNRITPGDIESGKNELK
                     RLCEESGRDFSTIGITVYGATPDPSIVSELFSAGAERIVLSIDSVPEKEALEQLEGFV
                     NDFL"
     gene            5983..7275
                     /locus_tag="QF838_03725"
     CDS             5983..7275
                     /locus_tag="QF838_03725"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HJO59831.1"
                     /translation="MPAYAILGAQWGDEGKGKIIDYISRRADIVARFSGGNNAGHTVI
                     NDLGDFSLHLVPCGVFSDGVMNVIGNGVVVDPDVLIEEMEALQRGGIDISHSLMISER
                     AHLIMPYHVMLDTLAERERGDFAIGTTGKGIGPAYSDKTSRTGIRAADLLDLEGLRHR
                     LEEVLPFVNRVLTKVYEVDSVSIDYILDKSRMWKDFLGPLIKPVGRYVNEVLDSGGTV
                     VLEGAQGILLDLDHGTYPFVTSSSPTIGGASTGLAIQPRQITNVLGIFKAYNTRVGSG
                     PFPTELFDDVGEGIRTIAKEFGTTTGRARRIGWFDGVLARYSNRINGYTSAVITRLDV
                     LDHLDSINICVAYKLDGEILDEPPGGVAALERCLPIYETLPGWDTPTAGISEASQLPI
                     NARVYVDRLEELIGCPVDIISTGPHRDHTIMVRDILAS"
     gene            complement(7296..8189)
                     /gene="dapA"
                     /locus_tag="QF838_03730"
     CDS             complement(7296..8189)
                     /gene="dapA"
                     /locus_tag="QF838_03730"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HJO59832.1"
                     /translation="MVDFGRLLTAMITPFDSAGNIDFPQARKLAKGLVASGTDGLVIG
                     GTTGESPSMSDDEKVQLFAEVKEAVGDTATVIAGTTDNNHRKSVELSIEAEKVGADGL
                     LLTVPAYNKPTQEGLYQNFKAISEATSLPGLLYNVPSRTALNMTTETTLRLAEIDSIV
                     GVKEASSDYVQITNIIDKAPDGFRVWSGNDDETFPIMCVGGHGVVSVASNLYGNQIKT
                     MMGLILEGNIESAATEHKRLLPIFKALFGVTNPIPIKYAVNRVGFNAGSVRLPLCDPD
                     DDFISTFNPVMDEYEADLPVS"
     gene            complement(8194..9228)
                     /locus_tag="QF838_03735"
     CDS             complement(8194..9228)
                     /locus_tag="QF838_03735"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HJO59833.1"
                     /translation="MDSCKIAVVGATGAVGQVFLDIAEQRNFPIDEIRLCASTRSVGK
                     KLKFKSQEIAVELCTPKVLDEVDFAFISASGDVSREIAPLAAKHGTVAIDDSSAFRMD
                     ADVPLVVPEVNVDDLSDHKGIVAIPNCSTTPLVMVLNPLKKKSSIKRVVVDTFQSVTG
                     TGTAALTEMEHQSKAVLQDNPITTKEYPHQIAFNVLPHIEPFQENGYTREEMKMLYET
                     QKILHDSNILVSATCVRVPVAVSHSEAIHIEFEDPITPNEVTEILGNEPGIRIVDDPF
                     SDVYPMPIDAAGGDDVLVGRIRKDISHPNGIAMWVVCDNLRKGAALNAIQIAEEMMKR
                     NVVSESNWRQ"
     gene            complement(9291..>9791)
                     /gene="dapB"
                     /locus_tag="QF838_03740"
     CDS             complement(9291..>9791)
                     /gene="dapB"
                     /locus_tag="QF838_03740"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HJO59834.1"
                     /translation="GLSQEDFEKAKSLAESNNTSIIIAPNFAMGAVLMSHLVKAAARF
                     FDYADLTEVHHEAKIDAPSGTALAIAQAAVEGKGSRFEAPLAEKELIADTRGGTHEGV
                     VIHSGRMPGRVAHHEMVFGSLGQTLTLRHDSINRESFMPGVIMAIKASETSNGLTVGL
                     DKIMGL"
BASE COUNT         2650 a         1962 c         2299 g         2880 t
ORIGIN      
        1 cttgatcagc cacatcagta tcaaaaatgc ttggcataat atattcgtta ttaatatggc
       61 tctctgagac tcccttggat atggcaaagg ctgcggccat tttcatttca tctgtaactt
      121 tttgtgctct tgcatctagg atgcccctaa acatgccagg gaaacacaaa gcattattaa
      181 cttggttggg gtaatctgat cttcctgtcg ccataacttc aacgagtcct tcgacttcct
      241 ccggccatat ttcaggatca ggattcgcca tagcaaaaat tattgggttt gtgttcatca
      301 aactcacgtc ttcaatttgg aggacttctg ggcctgatag gcctataaac acatctgcac
      361 ctgtcacagc ttctgtgatt tgcccagaga tattatgtgg atttgttatt ttggaatatt
      421 ctttccaaac tttgttattc ccgtaatctt tttgcctgtc taaaatccct aaccgatcca
      481 cgccaatgat attttttatg ccggctttga ttaaaagttt ggtagtggcc accccagccg
      541 ctcctactcc aactaatact acctttagtt caggtaaatt tttgttgacc actttgactg
      601 cattgtatag tgccgccaga accacgattg cagttccgtg ctgatcatca tgaaatacgg
      661 gtatggccag ttccttgtga agacgttgtt ctatttcaaa gcatctcggt gccgaaatgt
      721 cttccaggtt tatcgcccca aacccggggg atagcaattt tattgtgtct acgattttgt
      781 ttgtgtcgtg agtatctaga acaataggcc aggcatccac cccgactagt tctttcagta
      841 attgagcctt gccttccatt acgggtaaag cagcagcagg accaatatcc cctaatccga
      901 gtacagctga cccatcggtc acaattgcaa cggtattacc cttactcgtg agattccata
      961 cagattctgg ttgttcatga atggctttgc taactctcgc aacacccggt gtgtatatca
     1021 atgacatgtc atttctagtt ttaatgggtg ctttgctttg catttcaatt ttaccgccaa
     1081 gatgccttag aaagacttga tcggatacat ttactattgt taccccttca acgcttgata
     1141 ccgctttttt caatatctct gcatgagaat cgtcccgagc atttattgta atatctcgga
     1201 ccatgccgtt tcgtccggct gatacgatgt cgattgcccc aatatccccg ccagctgcac
     1261 taatgttggt ggttatttga cctagcatgc caactgtgtt gggatatgaa gttcttatcg
     1321 tgatactatg cgcggctcca ggttgccttc taacagtcaa gtgattacct cacgcatgat
     1381 actgtctcat tttatatggt tcgtaaatac tgcttcatac tatacccgaa tgaattttgg
     1441 ttagagagta ttaagtgagg ccacttccag cctctttatg tcatgaacag acttagcctt
     1501 tttaaggaat ataaggagga tatggccagg tcacgtgctt gatattcgcc atataatatt
     1561 gatggcatct tctttaacct tattggaatc tggtatggaa aatttttacg aacttcggac
     1621 ccaagcctaa agtcaaacct agtgacagct gatagaaata agcaatcagt ttagaaatct
     1681 cctaaattcg gactcagtgg agtaactaat aggtgaatgt tgatatgggt ctgagtgaga
     1741 tcggtaccag tgaattactg gaactgcttc atacgactgg aggctaacat ggcgtaacat
     1801 gtaaaagtta catcaaagat atgtctaggg ggcagatttg cataagtcaa aggctgttat
     1861 cgcaagaaat aggggagaga gggttgaaga ggttgagatt ttagttcctg aacccgggcc
     1921 caatgaagca ttgattaaaa tcaaggcctg tggcgtgtgc catacggatc ttcactatcg
     1981 tgaaggtgca atcaataatg aattcccttt tttgctaggg catgaggcgg ctggttatgt
     2041 agaggagata ggggaggggg taaccaatgt cacggtaggg gatttcgtga ttatcgcctg
     2101 gagatctccg tgtggtaggt gtcgctcatg tcttaaaggc aaaccctggt attgttttga
     2161 cagtagaaac gctgttcaga acatgtcact ggaggatggt tctactcttt cacctgcttt
     2221 gggcattggt gctttctcag agttaacgtt agttgacgca ggacaggcta ttaaggtgga
     2281 tgcagcggtt aattccaata cggcttcttt gctgggttgt ggtgtaatgg caggtcttgg
     2341 ggctgctata aatacaggat caattgggat gggagattcc gtagctgttt ttggatgtgg
     2401 aggtgttgga aacgctgcaa tagttggctc taagttggcc ggagcccaga aaataatagc
     2461 agtggacata gatgaccaaa aattggagtg ggccatggag tttggagcca ctcacactgt
     2521 aaatagccga agcactgatc cagtagattt tatccgtagt gttactgatg ggaatggaac
     2581 aaatgtaact attgaagcgg tgggtaaccc aaagacatat gaacaagcct tttatgccag
     2641 agaccatgca ggggttttgg tattagttgg agtgcctgat cccaccatga gaattgaact
     2701 tcccttgata gaagtatttg gacggggagg ttccctaaaa tcttcttggt atggagactg
     2761 ccttccttcc agggatttcc ccaaattcat tgatcttcac ttggatggaa ggataaattt
     2821 ggacaaattc gtaacggaga caatatctat gtcggaagtt gagactgctt ttgaaaaaat
     2881 ggaggctggg aaagtattaa gatccgttgt ggtttttgaa tgatctagtt cagggtagtg
     2941 acattttgaa gattgaataa ttcaaatgga aggagtttac atggtgataa cggttcgaag
     3001 tagaaaaatg gtttctggtt ctgttaatag ccttatgaaa acaattgatg acgggttcat
     3061 gcccatcatt agtgaggagc cgggatacat agcctattat gttgtcgact ccggtgatgg
     3121 actggtcacc gctgtaagta tatttgagga agaagaaacg agtgttaaat cgaataattt
     3181 ggcccaggag tggatatcta aacatttagg ggatttggtt cccggtaagg cccaagtaat
     3241 atccggacca gtaatggtac caagtcaaag ttaaacgaat ggagaaaatt atgccccgat
     3301 tgaaatatcc gcttgccgtt ccagttaagg caggcttaaa gactataaat ctatttgaac
     3361 ttagggctga gacttcactt cgagacaatg tcaatgattt tcgagaatat gccgatgttc
     3421 tttatagtgc tcacgctcca actaccatag gtgaagaacg attgaatatt gcagcaacag
     3481 atagtgactt tagaaacgag tgtattttgg tttttaaaga ttatattgat cgctgtgcaa
     3541 tgtttccaaa tattggacaa attaacatgc attttggtct taagaactgg gtttctgaga
     3601 cgcagccacg tggccaaaaa ggggattatg agacacacat tgaatcgata aggactcttt
     3661 cagactacgc gcgaacctca ggaattgaaa ttgttttgga gaatttgaat tgctattggg
     3721 cgctcaataa tatttcggat gatactgatt tcgctcaggt taactgggat aattccaatg
     3781 aggcctttgg aatgaatccg gaggaatgga ttgagatgtg cctggatgtt gatagagaca
     3841 atgtccaact ttgtctcgat accagccatg tgtcgaccta tggacacaga tttccagaag
     3901 aggagcgtgc gggaaagatt gaggcttttc tcaatcgtcc ggacctcatt acccatgtac
     3961 attggagtga caattatctt tatgacgtta gagggagaaa tgattcccat ctgtctgtgg
     4021 gcaagggttc aatacctaca gactttcatc ggcgggtcaa agctttacaa gcgaccattc
     4081 tcttagagca tttttacact aaagaagagt tacatgaaga acttgagtat attgaacgtt
     4141 tgtaattatt ttgggtaaat tcacttttgg gaataataca gcgtagatta aagcttttct
     4201 aggaacaaaa caaggtgatt tgaagggact ggtgccgaag gagggattcg aacccacaca
     4261 cccttgcgga tactacgtcc tgaacgtagc gcgtctaccg ttccgccact tcggcatttt
     4321 ctggtgggcg atagaggact cgaacctctg acctctaccg tgtgaaggta gcgctctaac
     4381 cagctgagct aaccgcccta attttgcccg gtaagaccga gccttagaga acagtttggt
     4441 ttctggctac ggctggattt gaaccagcga cctagcgctt atgaagcgcc cgctctaacc
     4501 cctgagctac gtagccatgc tagtcccaca gactaataaa atataatagc cgatataggg
     4561 ggtgtcaaac aatggattcg acaacgaaac cgaatataga agctgatgtg attttaaatg
     4621 agacaaacgc taaattgcat aacttgctga ttgggcttgc aagttctgtg gcaaattttc
     4681 ctggattctt aggtatggaa actgtccaag ccatagaagt agaacctatt gtcggttttc
     4741 ccgacagagg ttgcattgtc gtgaccccgg aagggttttt aaaggaactg atcctatcag
     4801 tactgcctgg agcatctact ttgggaggtt atgaacaatc agagcaattg aaagatttgg
     4861 atttgccacc ggaggaggaa acggtatacc tttaccgagc cataaaattg ctatccaagt
     4921 aggcctagaa tattgatttt aaaaagaggt atgtgcaaat gggtaatata gggataacta
     4981 catttccaac agattatgct attgatatag cagttttggc agcgaaagcg gaagaagctg
     5041 gattcgattc attgtgggtt gctgagcatc cagtattacc tgtgggtgca gaaacacctt
     5101 ggccgggtcc aggtggtgtg atacctaaaa agtatgccga tgtggccgat ccctttgtgg
     5161 ctttaggtcg agcttcggca gttacagata agctattatt aggaacaggt atttgcttgg
     5221 tgccagaaag aaaccctctt atactcgcaa aagaggtagc gactctagat atgtatgcca
     5281 agggcagatt tttgtttggt attggggccg gctggttaaa agaagaaacg gatattatgg
     5341 gaggtgattt tgcccaccgt tggactcaga ccagagaatc tgtgatggcc atgaaagaac
     5401 tttggaccac ggtggaaagt gaatatcacg gtaagtatta tgattttcct cctgtgtatt
     5461 cttttccaat gccagttcag cgcccacacc ctcccatata tctgggcggt catgcaaaaa
     5521 atgtatttaa aagagtggta agttggggcg atgcttggat gccgaatcgt ataacccctg
     5581 gagatattga gagcggtaaa aatgaactta aaagactctg tgaggaatca ggcagggatt
     5641 tttccacgat aggaataact gtttacggtg ctacccctga tcccagtata gtaagtgaac
     5701 ttttttcagc aggtgccgaa agaatagttt tatccattga cagcgtccct gagaaagaag
     5761 ccttggaaca attagaaggg tttgttaacg atttcctgta aaaatttggg aaatctacac
     5821 taattgaccc agtaacttct gttatacttc ggagtgaaat ctccctgtga agtgtccgtg
     5881 ttttgaggtc cggaccctca ttgaggagaa atttttttgc ccagaaatat ttgcctttta
     5941 taggcttaac aaaatgttga gataaattta ggagaattgc ggatgcccgc atatgccatc
     6001 ctcggtgccc agtggggtga tgaagggaaa gggaagataa tagactatat ttcacgacga
     6061 gctgatattg tggctaggtt ctctggtggc aacaatgccg gccacacagt gataaatgat
     6121 ttgggtgatt ttagccttca cttggttccc tgtggtgttt ttagcgatgg ggtcatgaat
     6181 gttataggga atggagtggt agttgaccca gatgttttga tcgaagagat ggaggcgttg
     6241 caacggggag gtatagatat aagtcattcc ctcatgatta gtgaaagagc ccatctcata
     6301 atgccttacc atgtaatgtt ggacacctta gctgaaaggg agcggggcga ctttgcaatt
     6361 ggtaccacag gaaagggcat tggccctgcc tacagtgaca aaacatctag aacgggtata
     6421 agggcggcag atcttttgga tttggaaggt ttaaggcacc gcctggaaga agttcttccc
     6481 ttcgtgaata gagtattgac taaggtgtat gaggttgatt ccgtatctat agactatatt
     6541 ttggataaaa gtaggatgtg gaaagatttt ctgggacctc ttataaaacc agtaggtaga
     6601 tatgtaaatg aggtattgga ttcgggtggt acagtagtcc tagagggagc acaaggaata
     6661 ctgcttgatt tggatcatgg tacatacccg ttcgtaacat cttccagccc caccattggc
     6721 ggtgccagta ctggtttagc gattcagcct aggcaaatta caaatgtgtt gggtatcttt
     6781 aaagcttaca atacaagagt tggttcaggg ccatttccta cggaattatt tgatgatgtg
     6841 ggggaaggaa ttcgcaccat tgcaaaagaa ttcggtacta caacaggaag ggcaagaaga
     6901 atagggtggt ttgatggggt gctggcgaga tacagcaaca ggatcaatgg atatacctct
     6961 gcagtaatta caagactgga tgtgctggat catttagact cgataaatat ttgtgtggca
     7021 tacaaattgg atggtgaaat acttgacgaa cctccaggag gggtagcggc cctagaaaga
     7081 tgtttaccaa tttatgaaac tctgccggga tgggatacgc ctactgccgg tatatcagaa
     7141 gcctcacaat tgccaattaa tgcaagggtg tatgtggatc gcctcgagga gttaattggt
     7201 tgcccagttg atattatttc gactggacct catagagatc acacaataat ggtaagggat
     7261 atcttagcct cctgaattaa gccgattggc aatatttagg aaactgggag atccgcctca
     7321 tattcgtcca ttacaggatt aaaggtcgat ataaaatcat catctggatc gcagagcggg
     7381 agcctgactg atccagcatt aaaccctact cgattgacgg catatttgat tgggatagga
     7441 ttggtaaccc caaaaagggc tttgaaaatt ggaagtaacc gcttgtgttc ggtggcagca
     7501 ctttcaatat ttccttcgag tatgagtccc atcatagttt tgatctggtt tccgtagagg
     7561 ttagaagcaa cacttactac tccatgccct ccaacacaca tgatgggaaa tgtttcgtca
     7621 tcatttccag accaaactct aaatccgtcg ggagctttgt caataatatt ggtgatttga
     7681 acataatcgc ttgaagcttc tttcactccc actatgctat ctatctcagc aagtctcagt
     7741 gtagtttctg ttgtcatgtt caaggctgtc ctagatggaa cgttgtaaag tagtcccgga
     7801 agagaggtcg cttctgatat cgccttaaag ttttgataaa gtccttcttg tgtaggcttg
     7861 ttgtaggcag gaaccgtcag tagtaacccg tcagccccaa ccttttctgc ctcaatagac
     7921 agttccacag attttctatg attattatcg gtggttcctg cgattaccgt ggcagtgtca
     7981 cctacagctt ctttcacctc ggcgaatagc tgtacctttt cgtcatctga catagaggga
     8041 gattcgcctg tggtaccgcc tatcaccaac ccgtcagtcc ctgatgcaac taatcctttt
     8101 gcaagttttc tggcctgagg gaagtcaata ttaccggctg aatcaaacgg ggtaatcata
     8161 gctgtgagaa gtcttccaaa atctaccatg ttcctattgc ctccagttac tttctgaaac
     8221 tacgttgcgt ttcatcattt cctctgctat ttgaatagca ttaagagctg caccctttcg
     8281 taaattgtcg cagacaaccc acatagctat tccatttgga tgtgagatat cttttcggat
     8341 tcttcctacg agaacatcat cgcccccggc agcgtctatc ggcataggat acacgtcaga
     8401 gaatgggtcg tctacaatcc ttatacccgg ctcatttccc aatatttccg tgacttcatt
     8461 aggcgttatt ggatcttcga attcaatgtg aattgcctca ctgtgactca cagcaactgg
     8521 tactctcacg catgtcgcag aaaccaaaat attggaatcg tggaggattt tttgggtttc
     8581 gtagagcatc ttcatttctt ctcttgtgta cccattttct tgaaaaggtt ctatatgagg
     8641 caatacattg aaagctattt ggtgaggata ttctttggtt gtgattggat tatcctgtag
     8701 aaccgctttg ctttggtgtt ccatttcagt cagtgctgcc gttccagttc cagttacaga
     8761 ttggaaggtg tcgacaacaa cacgtttgat ggaggatttc ttcttcagag gatttagcac
     8821 cattactaaa ggggtagtag aacagtttgg tatggcaact attcctttgt gatcagataa
     8881 atcatcaaca tttacttcgg gaaccaccaa gggaacatct gcatccattc ggaaggctga
     8941 gctgtcatca atagcgaccg tcccatgttt cgccgcaagg ggcgcaatct ctctgcttac
     9001 atcaccgctt gcagaaatga atgcaaagtc aacctcatcc aaaacttttg gggtacagag
     9061 ttcgaccgct atttcctgtg atttaaattt taatttttta ccgactgatc gagttgaggc
     9121 gcataacctt atttcatcga tggggaagtt tcgttgctcc gcaatgtcca agaatacctg
     9181 gcccacggct cctgttgctc ccacaacggc aattttgcaa gaatccaagt tttcctccgt
     9241 aaatttgaat attaaaccgg tggatgggtc gactgataaa atggagaggg ttataggccc
     9301 ataattttat ctagcccgac agtcaatcca tttgatgttt ctgaggcctt tattgccata
     9361 atcacacctg gcataaaact ttctctattt atcgaatcat gacgcagtgt caaagtttgg
     9421 cctaaggatc caaaaaccat ctcatgatgt gcgactcgtc ctggcattct accgctatgt
     9481 attacaaccc cttcgtgtgt tccacccctg gtatcagcga taagttcttt ttcagcaagt
     9541 ggagcctcaa agcgactccc tttcccctct acagctgcct gtgctattgc taaagcggtt
     9601 ccagatggag catctatttt cgcttcatga tgaacttctg ttagatcagc atagtcaaaa
     9661 aatctagcag ctgcttttac taaatgtgac atcaataccg ctcccatagc gaaattagga
     9721 gcaatgatta tgcttgtgtt attagactca gctaacgatt tagccttctc aaaatcctct
     9781 tgagatagac c
//
LOCUS       DASCNS010000104         4969 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000890563, whole genome shotgun sequence.
ACCESSION   DASCNS010000104 DASCNS010000000
VERSION     DASCNS010000104.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000890563"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            85..777
                     /locus_tag="QF838_03745"
     CDS             85..777
                     /locus_tag="QF838_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015344.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HJO59835.1"
                     /translation="MTFLNPLKTYFYHLFVMVRKDFLLEIRSKDVILPVFIFSLLVVV
                     TFNFALESTPNTANLIVPGILWISIIFGAMVGFNRMLLAETEGGAFQGLLVSPVGRDV
                     VFFGKVLSNMMFLIVIELILVPILIVLFNVSISPLNMLWISLPALLGISLCGMLFATI
                     SMNMRAKEVMLPILFLPVVVPIILGAVESTHSIMNGGNLDENFRWIALMIVFDVIYAV
                     LGPVSFTMIVED"
     gene            1038..2741
                     /locus_tag="QF838_03750"
     CDS             1038..2741
                     /locus_tag="QF838_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HJO59836.1"
                     /translation="MKKQDFVVRFAGEGGQGVVTSAEGLAQAMAQSGYNVQTFSTFPS
                     QIKGGPTWTQTRISTSPVLSSGDELDVLVAFNQYAYDNHKDEVADDGVIVYNSGEFEI
                     DASDKALAMDFDELAKQTGNNRAANFVVMGALAQLVDLPINILEEFNRQRYTRGRPSD
                     QQIIDSNNMALSLGSEEAKKSGFNLGELSDPIKPEGEQVLLNGNLAFSIGAAHAGLDV
                     FVGYPISPATPILLWMEQNLVGEGKFVHQSSSEIESITSIVGAGYAGKKAMTSTAGPG
                     FTLMGEGIGLAWMAEIPLVVIDVQRGGPSTGLPTKTEQSDLLTALYPGHGDMKMPVMA
                     PGTVEECFVAGVMSVNWAERYQGPVVVLSEMALSERRQNIPRPDLKSLVSEKREIYEG
                     TNGYHRYDGWELSPMPLPGGPGSYVANGSEHDAMGDTTHLPARHVQMTERRFSKLKLL
                     NDGTFESDNEHSNIALVPWGGSKGPALDAYNQLRSENKDLGWYYTMYIHPMPEKMLAA
                     LKEKELVIVPELNYLGQFGSVLRGEGINAVSLTQYTGLPFKVRELVSRINELIKDGVA
                     V"
     gene            2738..3604
                     /locus_tag="QF838_03755"
     CDS             2738..3604
                     /locus_tag="QF838_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO59837.1"
                     /translation="MTLKNPEYDFKWCPGCGDFGVRRALEVALSEYTTENEKPIDNTV
                     VVSGIGCSGNMVHMLEKEQPFGIHGIHGRSLPISMGIKMGNPDLNVVVVAGDGDFLGI
                     GMEHIAPQALRNLNVTAVVMDNGVYGLTKGQSSPTTDLGVITSSTPYGKVEDSLNPLR
                     TYLTVGVSFIASTISNKVKDMTGLMAQAMNHKGFSIVHVQSPCTEYNNTYEALKGNES
                     KGIKPLAYDIPEGHDPADIEAAQELLNTDGIPLGLVYRDSSRATFDQKIHETIGVKDM
                     KPIESVLDGFTI"
     gene            complement(3694..4125)
                     /locus_tag="QF838_03760"
     CDS             complement(3694..4125)
                     /locus_tag="QF838_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HJO59838.1"
                     /translation="MAEQIYFEDIEEGSEIPTLRKDPTTQQLVKYAGASGDYYQIHYD
                     KGFALNNNLPDVILHGALKNAFLGQLMTDFAGLGGELKKLSVQYRAMDVPGTPVFGKG
                     TVKKKYVQDGENFVECEIWLETHEGKTTTPGYGIATLPNKE"
     gene            complement(4136..4597)
                     /locus_tag="QF838_03765"
     CDS             complement(4136..4597)
                     /locus_tag="QF838_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024844.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HJO59839.1"
                     /translation="MADSVITQEMKDAIGVESEPVVNDVEKGAIIKFAQAIGDESPVF
                     NDEDAARQTKYGGLVAPPTFLRSMRLGEARVTVTSPYPANLDGGSEWEYFEPIRPGDR
                     ITVTQYLADVNERQGRLGNMLIMVRETKYVNQFGKVAALQRSTGISYQPTE"
BASE COUNT         1362 a          958 c         1158 g         1491 t
ORIGIN      
        1 tgttgcgaac aacatcccac acaatgaaat tcccagaagg gccggtaaag agatccaaag
       61 catattcaaa aggaatgctg gttgatgaca tttcttaacc ccctgaagac atatttttac
      121 cacctttttg taatggtaag gaaggatttc ttacttgaaa tcagaagtaa agatgtcatc
      181 ctgccagttt ttattttctc tctgttggtt gtcgtgacat ttaattttgc ccttgaatca
      241 acccccaaca ctgccaattt aatagttcct gggattcttt ggatttctat aatatttggt
      301 gctatggtgg ggtttaaccg gatgctgcta gctgaaacgg aaggtggagc ctttcaaggt
      361 ctgctggtat cacctgtagg aagggatgtc gttttcttcg gtaaagtgct atcgaatatg
      421 atgttcttga tagtaattga attgatactt gttccaattt tgatcgtgtt attcaatgtg
      481 tcgatatctc ctttgaatat gctttggatc tctttaccgg cccttctggg aatttcattg
      541 tgtgggatgt tgttcgcaac aatatctatg aacatgaggg caaaggaggt tatgttaccc
      601 attttatttc ttcctgtagt tgtgcccatc attttgggtg cagtggagtc gacacattcc
      661 ataatgaatg gtggtaattt ggacgaaaat ttccgatgga tagccctgat gattgttttt
      721 gacgtaattt atgctgttct gggaccagtc tcttttacta tgattgtcga ggattgacct
      781 gttaagaatt agacgggata ttttaagata aacaatatga atttagattg tgaacacaaa
      841 cacaaaattg acactataaa aactcggttg atatattttc tgatggtgtt gtacattagc
      901 ccttgaatag ctatttacac tgactatact tagatatgtt tttagggttg ggttggtgtg
      961 attcggtaca atgggcatta catgctttgg cagccgcaaa tggctgccat tgaaaagaag
     1021 tttttcggga ggataaattg aagaagcagg actttgtcgt ccgatttgct ggggaaggtg
     1081 gacaaggggt agttacatca gcagagggac ttgctcaagc catggcgcag tctggatata
     1141 atgtccagac tttctctacc tttccttccc agattaaagg tggcccgact tggacccaga
     1201 ctaggatatc aaccagccct gtgcttagct ctggtgatga gttagacgta ttggtcgcct
     1261 tcaatcaata cgcttacgat aatcacaagg atgaggtcgc cgatgacggt gtgatcgttt
     1321 acaactcggg ggagtttgaa atagatgcct ctgacaaagc attagcaatg gattttgatg
     1381 aactagccaa gcagactggt aataaccgag ctgcaaactt cgtagtaatg ggagcactgg
     1441 ctcaattggt ggaccttcct atcaacatcc tcgaagaatt taataggcaa cgctacacaa
     1501 ggggacgtcc tagcgatcag caaattattg attctaataa catggctctt tcattggggt
     1561 cggaggaggc taagaagagc ggattcaatt taggagaatt atccgatcct atcaagccag
     1621 aaggagagca agttctcttg aatggtaacc tggcttttag cattggcgca gcacatgcag
     1681 ggcttgatgt atttgttggc tatccaattt ctccggcaac tccaattctc ctgtggatgg
     1741 agcagaatct ggttggagaa ggtaaatttg ttcatcaatc ctcatctgag attgaatcca
     1801 tcactagtat cgtaggagcc ggatacgctg gtaaaaaagc catgactagt actgcaggtc
     1861 cgggatttac cttgatgggt gaagggatag gcctggcttg gatggctgaa atacctttgg
     1921 tggttataga cgtacagaga ggtgggccgt caactggatt acctacaaaa acggaacagt
     1981 cagatttgct tactgcttta tatccgggac atggagatat gaaaatgcct gtgatggccc
     2041 ctggcacggt ggaggaatgt tttgtggctg gagtcatgtc tgttaactgg gcggaaagat
     2101 accaaggtcc ggtagttgtt ctgagtgaaa tggccttatc agaaagaaga cagaatattc
     2161 cgagacctga tctgaaatct cttgtttctg agaagagaga aatctatgaa ggtacaaacg
     2221 gctatcacag atacgacggt tgggaattat ctcctatgcc attgccgggt gggcctggca
     2281 gctatgttgc aaatggaagc gagcatgatg ccatgggaga cacaactcat ttgccggcac
     2341 gacacgtaca gatgacggaa agacggttca gcaaactgaa gcttttgaat gatggtactt
     2401 tcgaatcaga caacgaacat tcgaatattg cacttgtccc ctggggaggc tctaaaggcc
     2461 ctgccttgga tgcatataat caactgagat cggaaaacaa agatttgggt tggtattaca
     2521 caatgtatat acatccaatg cccgaaaaaa tgttagctgc actgaaagaa aaagaattag
     2581 tcattgtccc cgagttgaat tatttgggtc aatttgggtc tgtgcttaga ggagagggga
     2641 ttaatgcggt aagtcttacc caatatactg gactgccctt taaagtgcgt gaacttgtat
     2701 ctagaattaa tgaactcatc aaggatggtg tagcggtatg acattaaaaa atcctgaata
     2761 cgacttcaaa tggtgtccgg ggtgtggaga ctttggagtg aggagagcac tcgaagtggc
     2821 tctgagtgaa tatacgactg aaaatgaaaa accgatagat aatacggtag tggtgtctgg
     2881 tataggctgc tcaggcaaca tggtacatat gttggaaaaa gaacaacctt ttggaatcca
     2941 tggtatccat ggaaggtctt taccaatatc tatgggaatc aagatgggaa atcctgattt
     3001 aaatgttgtt gtagttgccg gcgatggaga ctttttgggt attggaatgg aacacatcgc
     3061 cccacaggct cttcggaacc taaatgtgac ggcagtggtg atggataatg gagtctatgg
     3121 tcttacgaaa ggacagagct caccaacaac tgatctggga gtgataacaa gctccactcc
     3181 atatgggaag gtggaggatt ctttaaaccc ccttaggact tatttaaccg ttggagtgtc
     3241 ctttatagct tctactatat ccaataaagt gaaagatatg acgggattga tggcccaagc
     3301 tatgaatcac aagggatttt ccattgtcca tgtgcaatct ccttgtacag agtacaacaa
     3361 tacttacgag gcactgaaag gtaatgaaag caaaggtata aaacctttag cgtatgacat
     3421 ccccgaaggg catgacccag cagatattga ggcggctcag gaattgttaa atactgacgg
     3481 aataccacta ggattggtat atagagattc cagtcgggca acctttgatc aaaaaattca
     3541 tgagactatt ggggttaagg acatgaaacc tatcgagagt gttttggacg gatttactat
     3601 ttagggtaca cggatttcga cacttaactg ataaaaaata cccgcttgag aataactctc
     3661 aagcgggtat tttttacttt ctgtggggga aggttattct ttgttaggaa gtgtggctat
     3721 tccatatcca ggggtggtgg ttttaccttc gtgagtctca agccatatct cgcattcgac
     3781 gaaattttca ccgtcttgga catatttttt cttcacggtt ccttttccga atacaggagt
     3841 accaggaaca tccatagctc ggtactgaac actcaacttt tttaattcac ctccaaggcc
     3901 agcaaaatct gtcattaact gaccaaggaa agcattcttg agtgcgccat gcaaaatgac
     3961 atcggggaga ttgttattaa gtgcaaatcc tttgtcataa tgaatttggt agtaatctcc
     4021 tgaagcaccc gcgtatttta ctaattgttg ggttgtggga tcctttcgaa gagtagggat
     4081 ttcagatcct tcttctatgt cttcaaaata aatttgttca gccatttttg tactcctatt
     4141 ctgttggttg gtagcttatc cctgtagatc gctgcaaggc tgctactttg ccaaattggt
     4201 ttacatattt agtttctcta accattatca acatatttcc taaccttccc tgtctctcat
     4261 ttacatcagc taggtattgt gtaacggtaa tacgatctcc gggccttatt ggttcgaaat
     4321 attcccattc acttccaccg tccaaattgg cagggtatgg actagtcacg gtgactcttg
     4381 cctcccctag tctcattgac cttaaaaaag ttggaggagc tactagccca ccatattttg
     4441 tctgccgagc ggcatcttca tcattgaaaa ccggactctc atctcctatg gcttgggcaa
     4501 atttaattat tgctcccttc tccacatcgt tgactaccgg ttcggattca actccgatcg
     4561 cgtctttcat ctcttgtgtt attactgaat cggccacttc gtctctccca ttaaccttta
     4621 tgaattactt catagatgag tttgattttc tgaaagttta tggagactgg cagaaggtgt
     4681 caagaagata ggggaatgag gagttatcta ttggcatatt cctcaccttc tataattagg
     4741 ctttcgatca aacggtttcc aagaatcgga taaactataa atctttttgc gcacttattg
     4801 ataatctttg tagataaagt tcgctaattg atcaggtcta taggtgtcaa aatatttctt
     4861 gtctttgggc ccccaatcag tatcccagcc agctacagtc gtttcatctt ctccttgtga
     4921 gcgatctcta gccttcgcac gtttaatggc gaaatcttga ggacagtca
//
LOCUS       DASCNS010000105        25422 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000891479, whole genome shotgun sequence.
ACCESSION   DASCNS010000105 DASCNS010000000
VERSION     DASCNS010000105.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 25422)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25422)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25422
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000891479"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="QF838_03770"
     CDS             <1..187
                     /locus_tag="QF838_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59840.1"
                     /translation="DGITGQVFGTSGWALSLYSPRHVIKSIHKDGRWTLDELDQLIPI
                     SLGSGLINPVPVEPPRN"
     gene            265..945
                     /locus_tag="QF838_03775"
     CDS             265..945
                     /locus_tag="QF838_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HJO59841.1"
                     /translation="MNWLDFVILGILIASMLWGLKTGIFVAGIYGIAILVGWRISGAV
                     SQFVGEFIGDALGFNSSSAKALSELSGINLGVDTIATVAAFVVVLILLLIITNIVLKF
                     VGPLLAVLDIATLGMGRIAGIVLGLIIGVIISFITISGLTRLAYDFHEIPELPGSSLI
                     SAGEVISIEGIDAKIENTRTSIETALLDSRIAPVFVNILEITPDNALGIIPSDYMTAI
                     KILDSKLD"
     gene            952..1479
                     /locus_tag="QF838_03780"
     CDS             952..1479
                     /locus_tag="QF838_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HJO59842.1"
                     /translation="MATATAPTIESPVLVLNQNYQPLNICSVRRAIVLMGRGKAELII
                     NGRGEIRSSSAAFPMPSVVRLYYMVKKPMVERRLSRQALFYRDNFTCQYCGISTKKLT
                     IDHIRPRSRNGKHEWENVVSACMTCNHNKAEKTPREAQMTLLRAPLAPKPNPYYIFYH
                     RKLEDEWSPFIPWKN"
     gene            complement(1499..2533)
                     /locus_tag="QF838_03785"
     CDS             complement(1499..2533)
                     /locus_tag="QF838_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59843.1"
                     /translation="MAWKTIGQPNSIDRISRAVVLDRLSHAYLICGMDGIGKVTFAQD
                     IAKVANCLEMPKSSGPCGSCNQCIRIDSHNHTDVFIYDVKKDLDEVGRTTTMVTIDQL
                     REDFLRQVHRKPYEGRTRIFIISSIESMRSEQSNILLKTLEEPPDDVVIILLARDASN
                     LLSTIVSRCQVLNLDPVGELEILEYLGECELGPEVPIQEIAKLSRGRPEWAYRAAINP
                     EILHILEDKLNQFVGCLIGSLEQRFKLSKDLSTQFFRDRDGVYEFLDIAMTWTRDVLL
                     SSSERAGDIVNISHKERIHEFSKMLSTTDILNILKLIRMTLGNLRKNVTPSLVLDNFM
                     LKLPVATSLT"
     gene            2690..3616
                     /gene="htpX"
                     /locus_tag="QF838_03790"
     CDS             2690..3616
                     /gene="htpX"
                     /locus_tag="QF838_03790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HJO59844.1"
                     /translation="MQRNSIGKDHQLTIRMLATGLMLLVAYLALFGLLLISGLNPIFV
                     FVIGIGMVFFQYFMSDKLVLKTTKAKAVTKEEQPKLHEMIERLSKIGGIPKPRTVAIM
                     EMEAPNAFATGRSPKHATVAVTTGLLSKLNDKELEGVIGHELAHIKNRDVMVMTWASL
                     IVVVAGFLMQMLFWMSLFGGFGGHRRQGNGGQAMAMMMVVYIGVIIIYFISQLLISTL
                     SRYRELAADREGAIMTGSPTNLASALKKISGQIAQIPEKDLRQVEHANAFFIIPALQG
                     NAIASLMSSHPPTEIRVARLMEMQQQNWHLLR"
     gene            3627..4205
                     /locus_tag="QF838_03795"
     CDS             3627..4205
                     /locus_tag="QF838_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59845.1"
                     /translation="MRLFRTLFKKPDSPESNESDILSTVLRITNRDTNGKKDINLSGE
                     STLLLNANDHPFFTDIKSSLKELDKELFMSTMRTPLQGLKSETYNSCWIIFESTNNQK
                     MVDDINAISSHIANLGLGENMTAAVFKGTIGDQSFYCICNYRTSKFYPLVPLGHDMRD
                     NELEMKIGDRIASIGIPLEPHENWYSLSDVPI"
     gene            complement(4219..4404)
                     /locus_tag="QF838_03800"
     CDS             complement(4219..4404)
                     /locus_tag="QF838_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59846.1"
                     /translation="MPFLFTILSHHSEPGDPDLMSWVIDTVKHLVGGDSTLALLVLFL
                     MILMVPFGIVAAYKLFF"
     gene            4515..6413
                     /locus_tag="QF838_03805"
     CDS             4515..6413
                     /locus_tag="QF838_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HJO59847.1"
                     /translation="MSLLGASQLNLHYADIEIFSDVTLQVNEKAHIGIVGPNGSGKTS
                     LIRVLLGIQDYDGGDIFKSDSLRVGYVPQTALQSGGGTLKDQIGQAFQHLIQLEKDME
                     TNAFAIQDSEGVERTQAEKKYSTLLDKYEALGGYDYQNRMDRVVDGVGLSDTNLDTLV
                     SDASGGERTRAALATALLADPDLLVLDEPTNYLDFKGLEWLEDFLNRSANAFMVVSHD
                     RFFLDRVASDIWEIDNCRLKTYRGNYSKYKLLKSEEDARFAKEYIRQQEYIKREQDFI
                     ARYHAGQRSKEARGRAKKLGRIERIEAPSTEQNVSITSGFATRTGKTVISTNGLEVGF
                     STEQHSVKLFEVPDIEIERGTTTAIIGNNGIGKTTLLKTIIGSLPALSGSSNLGHNVK
                     VGYFRQGSDEIPSHMTVLDALLEIKNLSIGEARSFLARFLFTGEEIYDQVSSLSGGER
                     GRLALSRLLITEPNVLVLDEPTTHLDILSREALEDMLQVFGGTTIFVSHDRHLISLLA
                     DNLLIIAEGSVRHFAGSYQDLLKESNSDSSTKEKEKPVQLPPPKKSSRSKPIPKLNGT
                     NPNRNAASRKEDHEKLISDLESQIENLEKALQEASNTGNLDRITVLGQEHEDLNIKLD
                     RAISRWVE"
     gene            complement(6432..7028)
                     /locus_tag="QF838_03810"
     CDS             complement(6432..7028)
                     /locus_tag="QF838_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024214.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HJO59848.1"
                     /translation="MTSAGFSTRMGTVKALLPWRDTNLVCHQVRALLKGGCGHVVIVL
                     GYECARISEEIQKEFPENSPITLTCNVDYETGRASTIRQGILSSPKDSSGFLLIGVDQ
                     PTSGHIVARIIKEHEATRMPISSPRYKGKGGHPVLFANTLKDELLEINDETLGLKPVF
                     ENYRSRLNRIDFKTDQVRLDLNDYESYLHGYELFGNKT"
     gene            complement(7057..8202)
                     /locus_tag="QF838_03815"
     CDS             complement(7057..8202)
                     /locus_tag="QF838_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024869.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HJO59849.1"
                     /translation="MHEVFSDAINGLSRGEKVVVATVVRTKGSTPQKPGAKLLVREDG
                     SGTGTLGGGCVEGDIWFAAKQLMQAGGGTEYREYELNEDLAAEDGLVCGGTMYFLIDP
                     VYSPDSYLPYASEIDKAYSGVGAVALATVVRTGKKGYSKIGDKLFVRENGESEGSIGD
                     DSEDDQAKNKAFELMIHGRNEYVTTKSGTEYFIEAYTTPPQLVICGGGHVARALASLA
                     KPLEFRLFITDDREEFANDDRFPEADIIISETPEQALPQLPINPNTFIVVATRGHRYD
                     NVALLAAAKTSAKYVGLMGSKRKTILIYEDLIREGLEYDRLNEIRSPIGLDIRARTPE
                     EIAISIMSEILMFRLGGSGVAMKLDKKQIDRIKAKVNRNSKTLAETT"
     gene            8369..9028
                     /locus_tag="QF838_03820"
     CDS             8369..9028
                     /locus_tag="QF838_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HJO59850.1"
                     /translation="MVNTETQKIPLFPLSTVLFPGGTIPLQIFEDRYHSMVQDCLDTD
                     SKFGIVLIKEGQEVGGNAVPHKVGTLAKITKIRKLSQNRLYLTAVGEKRFNIVELIQN
                     KAYLEGLVEIQNPPPEQDVAEQSLKKAKTAASRHLKAIVSMNGGWVNKAETPLPTNPD
                     QLSFFLAQLIQGTNEIRQTILEETSIERRIEICLFHLQVESKNISSSLNTDLLFKFSR
                     N"
     gene            complement(9048..10241)
                     /locus_tag="QF838_03825"
     CDS             complement(9048..10241)
                     /locus_tag="QF838_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HJO59851.1"
                     /translation="MAFDFQNILATHVPETTRVTPAGKGKYDFAVAYPDPVSLPHAEI
                     LESLEEALSESGSELAVYPHPQGNTPLRQYVAEKIERDRGIKVTENDIILGNGSGQPI
                     DMICAVLLNPGDVVITDSFVYGGTLNTLRRHFTDIRGVASDESGMDPGALDKEIEKAI
                     KSGHKPKFVYLIPTFQNPQGWTIPESRRKELLTVTQKYGVPILEDDCYADNRYDGDLP
                     TSILSLDDTSSVMYVGSFSKTIAPGMSLGYMTAPQLILDRAMGVKSGRVSEFTAMAVE
                     KYARKYLDAHIEEINNIQRTKRDAMLSALGENFGTGAKWSDPAGGLYIWVKLKEGSDM
                     VALNKRSVEEIDVGFHPGTNYSPDGTSGKDHIRLCFGYNQPDEITEGISRLANFLSKE
                     GALDS"
     gene            complement(10338..11054)
                     /locus_tag="QF838_03830"
     CDS             complement(10338..11054)
                     /locus_tag="QF838_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HJO59852.1"
                     /translation="MTEVLCYEDSYLQEFEGTVTDVTDSGVVLDRTSFYPGGGGQPCD
                     TGVIEWDGESSQIYQVSRSEGKLVHKVNGPIPDLGNSVKGKIDWIKRYQLMRTHTALH
                     ILCGVVWRDFGAQVTGGNMTPLQARMDFELKEMSSGFASDIEILINQEVTAERDIKIS
                     NLPREEAFKIPDLIRMKINKLPPAISEVRIVDISGLDLQADGGTHVRNTREVGKIRVV
                     GHESKGRINKRLRIAVEDWN"
     gene            complement(11073..12254)
                     /locus_tag="QF838_03835"
     CDS             complement(11073..12254)
                     /locus_tag="QF838_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HJO59853.1"
                     /translation="MDSLRYKAAIVGASETTELGSIPDKTAFQLHVDASVNAINDCGI
                     DKNEIDGIATTMSPAALAHYLGIVPKWIDNTQVGGTSFLVHVRHAAAAIASGLCETVL
                     VAMAESGRNRVGEQTGRIGDVATMSGGRRDSSFPGQFESIYGVAGPTTQFGMGVLRYM
                     KETGLTHEQLASVPVAQRKWANKVPRAMYGDIITIEDVFNSRMICYPFHLLECCLVTD
                     GGGALIITSAERAKDFSTKPAYIMGTGESVESPIVSQMYDLTTSAAFKTSSKKAFEEA
                     GVTHDDVDHLMVYDAFAHLPIYGLEDLGFVKRGEAGSFIEEGNTSPGGTLPMNTNGGG
                     LSYTHSGMYGMYAIQESVRQVRGEAAHQVDGVKTSFCQGVGGMFMAAGSLVFTNEEPH
                     R"
     gene            complement(12297..12710)
                     /locus_tag="QF838_03840"
     CDS             complement(12297..12710)
                     /locus_tag="QF838_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HJO59854.1"
                     /translation="MSSGYNKPIPVPTPESDVFWEKAKQHELWIQRCLNCDKPFFYPR
                     MNCPDCLSDNLEWFKTSGLATLYSYMINHRPVPGFEEDAPYAIAIVQLDEGPRMMTNI
                     VGIENTPENLILDMKLKVVFDDINDEIAIPKWKPA"
     gene            12914..15766
                     /gene="uvrA"
                     /locus_tag="QF838_03845"
     CDS             12914..15766
                     /gene="uvrA"
                     /locus_tag="QF838_03845"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HJO59855.1"
                     /translation="MSPKSGPIKNIVVKGAREHNLKNVDVTIPRNKLVVITGVSGSGK
                     SSLAFDTIYAEGQRRYVESLSAYARQFLGRMDKPDVDYIEGLSPAISIDQKGTSHNPR
                     STVGTVTEIFDYLRLLFARVGKPHCYKCGRLVQKQTVQQIVDSIAGLPVSSRIQILSP
                     VVRRRKGEHKEVFESARKSGFVRVRVDGKIRDLSDNFELDKQKWHNIDIVVDRLVITE
                     KMDMVRISDSTEAALKQAEGIVLVDISGKDEMIFSEEFSCVYCNVSLGEIEPRTFSFN
                     NPHGACSTCTGLGFKMVVDPDLVIPDKTKTIEDGAIEGWSKGGSLSSWHISQLNSLSE
                     IDSFRIDVPIQELEPETMDMILNGTKTKIKMSYRSRRGRIFRWNRRFEGVIGNLERRY
                     TETESEKVRKDIERYMTSLPCQGCSGQRLKPESLAVTIGDETIMSVSDKSISEAADWI
                     ALLENPKILSNRDKSIASQILKELSGRLQFLLNVGLDYLTLSRTASSLSGGEAQRIRL
                     ATQIGSGLMGVLYVCDEPSIGLHPADDYRLINTLKGLRDVGNTVLIVEHDEAIMRAAD
                     HIVDLGPGAGEHGGEIVASGTVSKIMSSKNSLTGQYLSGAKVIDTPQTRRLFNGKYLE
                     IEGAEQNNLNSIDVTIPLGILVCITGVSGSGKSTLINEILYKRLSQHFYRAKDRPGKF
                     KSIKGLENIDKVVNIDQSPIGRTPRSNPATYTGTFTPIRELFAQVPEARSRGYKPGRF
                     SFNVKGGRCEACSGDGYINIEMQFLPDVSVPCEECQASRYNREALEIEYRGLSISNIL
                     QSTVTEALTIFSNIPRIKTKLQTLQDVGLGYIKLGQPATQLSGGEAQRVKLATELSKR
                     ATGQTLYILDEPTTGLSFEDCSQLLRVLQRLVDAGNSVVLIEHHLDLIKSADWIIDLG
                     PGAGDKGGNLVYEGTPENATRHEGSYTGKYLKNILP"
     gene            16079..16957
                     /locus_tag="QF838_03850"
     CDS             16079..16957
                     /locus_tag="QF838_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HJO59856.1"
                     /translation="MKWIDYENAKTVDEAIKILSQSGGKARPMAGGTDLIVQLRNKDP
                     RTDADVVVDIKDIPDLNVLQCDPKTGLTIGAAVPLYKIYNDDNVKKYFPAIIDSASLI
                     GGVLIQGRASFGGNLCNAAPSGDAIPNLIAHNGIATIAGPNGTRDVPVEDICTGPRQT
                     SIGNDELLISINLPVPAAGFGASYIRFTPRNEMDIAVVGAGVSVNISEGKFTAARVSL
                     ASVAPTPIYVENAVNALVGETVGDAAIQRAANAAREAAKPISDMRGTAEYRKHLCEVL
                     TRRALNAAIERAKEGK"
     gene            16957..17439
                     /locus_tag="QF838_03855"
     CDS             16957..17439
                     /locus_tag="QF838_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014880037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HJO59857.1"
                     /translation="MTTKTHVQTTINGEPVDFLCEPRQSLLECLRDVVGLTGTKEGCN
                     DGNCGACTVKLDGRIVTSCLVLGVEAQGADIETIEGVAEGTNLHPLQLAFLENAALQC
                     GICTPGFIVSAKSLLDQNPNPTEHEIRFWLANNLCRCTGYDKIVRAVQDAAKKMQEAS
                     "
     gene            17441..19708
                     /locus_tag="QF838_03860"
     CDS             17441..19708
                     /locus_tag="QF838_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HJO59858.1"
                     /translation="MTSSDSTDYKVLGTRPIRHDGADKVTGRAIYGPDFDTAGLLHGK
                     ILRSPHAHARIISIDTSEAEALDGVMAVVTGADWPNNSNGTSMVEDGTGTEIESNSNW
                     LRENLLAGDKVLYAGHAIAGVAAASPHVAEEALALIEVKYEVLEPVLTIQEAMAKNAP
                     ILHEGLTTQELGEDSGVVSNTADHFQHTKGNIEKGFEAADVVVEREYNTVMVHQGYIE
                     PHNGTALWNTDGRLHVWCSTQGSFVVRDSLSDLLDIPVSQIRVTPTEIGGGFGGKIPI
                     YVEPVAALLSKKTGSPVKIVMSRKEVFEGSGPTAGSNIKIKLGAKSDGTITAAHAWMA
                     MEAGGYPGSPVGAAAQCVFAAYDIENALVDGYDVVVNKPKTAPYRAPGAPNAAFGMEQ
                     AMNELAEMLQMDPIDLRLKNVATEGTRRVDGPTFRRIGAKEVLETMRDHPHYQAAKTE
                     GHGRGIAIGFWFNIGFDSACNIAVNSDGSINLTEGSTDIGGSRTSIAMHAAEVLGIPV
                     EDVRPSVVDTDTIGFTAVTGGSRTTFATGWAAYEAAQDVKKQSIERAASIWDVDSDTL
                     NMEDGVIKSKTDSELKMTFKELASQMSDTGGGITGRASVNPKGVGGSFAGNMVDVSVD
                     DETGKVTIERFTCVQDVGKAIHPSYVEGQMQGGSAQGIGWALNEEYFMNDQGNMANSS
                     LLDYRMPTSLDLPMIDTAIVEVANPGHPFGVRGVGEANIVPPTPAIANAVSDATGNIM
                     TQLPLTPGAVLDSGK"
     gene            19712..19975
                     /locus_tag="QF838_03865"
     CDS             19712..19975
                     /locus_tag="QF838_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HJO59859.1"
                     /translation="MPIIFVPSLMQKLCNGEQKLLIEGTNLRQVIDNLDLQYTGFKDR
                     LVEDGKIKPNISVAVDGEITPLGMIQKVDTNSEIHFLPAISGG"
     gene            20032..20889
                     /locus_tag="QF838_03870"
     CDS             20032..20889
                     /locus_tag="QF838_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59860.1"
                     /translation="MKPNQRGPKSRFLDNKTNLTHYIDWQGEEQTTLLIHGDMRTSRS
                     FDAVAKNLSKDSHVLAMDLLGHGDSTWLDRGYRFSDRSDDIENFITGTNLSKVHAVAH
                     STGAVALAMYVANSPERFEKLVLLEPMMIVDRKFQRMVSDRGNRPRTTWKDHDELKDL
                     LGKHEVTSKWHPDVIEDVVNHETFLNEDGRLDMKWSSSTLAWEERENDYLDLEPILKS
                     ISLPILFVVGETRIGGFEKAFQLESSLQNMQTVVISNTGHNMYMERPEAVAEVIRKFR
                     NSSRLPSRV"
     gene            complement(20910..22148)
                     /locus_tag="QF838_03875"
     CDS             complement(20910..22148)
                     /locus_tag="QF838_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO59861.1"
                     /translation="MKITNIETFIVDAGWRPWGFVKVETDEGITGWGECSDGRSPRGV
                     QGTIEDLKPLLIGTDPREFENRFWDMYRGTRQSPGGIAAKALAGLDCAFIDIKAKSLG
                     ISVPELFGGPTRDKVRVYWSHCGTSRTNNYELIGVPPLRTWDDVTALGKEVVDRGFTA
                     LKTNIIGPGDPAQHFGGGFGGGRGSTDQVVTKGVLRHVEKLIGTFRDAVGPDVDINLD
                     LNFHFKTEACLRIAQVLEQFDMLWLEIDMYQPESIRQIKDSTTTRICTGENLFYMRDY
                     LPYFQQHAADMFMIDVPWNGFSQSKKIGDLAEVFQHNVAPHNYYSHLSTFVSASLCAS
                     LPNIRIMEIDIDDVPWKDDLVTNKPEIIDGYMTIPSAPGWGTDINEEVARAHPWKGPK
                     GSYGSPAASPSEWTFGLPSF"
     gene            complement(22171..23736)
                     /locus_tag="QF838_03880"
     CDS             complement(22171..23736)
                     /locus_tag="QF838_03880"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HJO59862.1"
                     /translation="MNTTEFLNITSLIVPDRTAIVFDGKRFSFQELEERVKRLANALS
                     GMGVGAGDRIATMQVNCNENIETYFAAAKLDAVYVPLNFRSRPEEIEYMINDSKPKVL
                     ITGERYVSMVDEIKPDLTSVAGYVVLDDASEEGWESYEHLLSVSSDEDLYPTGDEDDL
                     TMVMFTAGTTGSPKGVMLSHDSFASYILANVSPPELESEEKNILTVPLYHIAGIQAVM
                     AAIYGGRTLIIQRQFEPLEWMALVQEERANRAMMVPTMLKMLMDHQEFEKFDLSTLEV
                     ITYGAAPMPLEVIKRAISKFPGTFFINAFGQTETAATITMLPPEDHVLEGSEEEIEKK
                     LNRLTSIGKPLSDVDVRIVNDEGGDVNVGETGEIVAQGPRLMKGYWNQQEATAEALRG
                     GWLYTGDLGYFDEDGYIFLSGRAKDFIKRAGEMVSPEEVEQVLHSHPSIDEAAIIGVP
                     DIDWGERVRAIVVAKIGATIDEEEVMAYCKDRMASFKKPESVVVVEELPRNPLGKVLK
                     RVLREEFSYPLEN"
     gene            23953..24768
                     /gene="greA"
                     /locus_tag="QF838_03885"
     CDS             23953..24768
                     /gene="greA"
                     /locus_tag="QF838_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HJO59863.1"
                     /translation="MASNNEMNLLEAVGEYTGSLKEKDKSQAAIREINRFVKHFQGER
                     LLASITPSQIGGYAEQVAKNTMSEEAVEGLQAVRKFLAYIHKQEITDMNLATHFRVRK
                     SKGNSRTGRGRSVSQRGGQEMTQEGHDQLTSEKDLLESNRVSISESIQTAAEDGDVRE
                     NAPLEAAREQQGREEARIKEIEGMLRSAIIVDSSGKGAKTVRVGTTIVIEDVDKKKKS
                     KYTLVSPSEANPLEGKISDASPLGKALIGKRSGQKAVAVTPKGQANLKILDIN"
     gene            24849..>25422
                     /locus_tag="QF838_03890"
     CDS             24849..>25422
                     /locus_tag="QF838_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJO59864.1"
                     /translation="MVITSKASDTQELVAELQKHVSGEVRFDKMSRALWSTDASIYQI
                     EPVGVVLPRNDEDVIAVIETAHKYGVTVLPRGGGTSLAGQTVGESVVVDFSRYMKSMG
                     EVNVEEGWVKTQPGIILDELNAQLSPHGVMFTPDPSTSSRGNVGGALGNNSCGAHSIL
                     WGKTVDNVYDLNVVLSNGDRTKFEHLTGDTL"
BASE COUNT         7672 a         5495 c         5339 g         6916 t
ORIGIN      
        1 ggatggtatc acaggacaag tttttggtac cagtggctgg gcattgtcgc tttattctcc
       61 acggcacgta ataaaaagca tacataaaga tgggagatgg actttagatg agttagatca
      121 gttgataccg atctctctag gaagtggtct cataaaccca gtacccgttg aacccccacg
      181 aaattaggta gcccaatatt tcacgagccg tatgctaaac tgcacctact tccttggaaa
      241 gcctaatttg aagaaggtaa gaatatgaac tggctagatt ttgtcatact gggaatccta
      301 attgcgtcca tgctttgggg tctcaagacc ggaatatttg tggccggcat ttatggaatc
      361 gcgatactgg tgggctggag aatttctggt gccgtttcac agtttgtcgg agaatttatc
      421 ggagatgcct taggtttcaa ttcttcttca gcgaaggccc taagtgaatt aagtggaatt
      481 aatctgggtg tcgatacgat tgcaactgtt gcggcttttg tagttgtgct cattctctta
      541 cttatcatca ctaatatagt cctaaagttt gttggacctc ttctagcagt tttagacatt
      601 gctacccttg gaatgggtag aatagccggc attgtacttg gattgataat cggtgtgatt
      661 atctctttta tcactatttc aggtcttaca cgactggcat atgactttca tgagattccc
      721 gaattgcctg gctcatctct tatttctgct ggagaagtca tttcgataga gggtattgac
      781 gcaaaaattg aaaataccag aacctcaata gaaactgctc tactagactc cagaatagct
      841 cctgtttttg taaatatttt ggaaattaca ccggataatg ctttgggaat tatcccctct
      901 gactacatga ctgccataaa aatacttgat tcaaagttag attaaacagg gatggctaca
      961 gctacagctc ccacaataga atcacccgtg ttagttctga accaaaatta ccagccatta
     1021 aacatttgca gtgtgagaag agcaatcgta ttaatgggca gaggaaaggc agaacttata
     1081 ataaacggtc gaggagaaat aaggagttca agcgcagcgt ttccaatgcc gtccgtggtt
     1141 agactctact atatggtcaa aaagccaatg gttgaaagaa ggctatctcg gcaagcatta
     1201 ttttatagag ataatttcac atgccaatat tgcggcatct caactaagaa gctgactatt
     1261 gaccacatta gacctcgttc aagaaatggt aaacatgaat gggaaaatgt tgtgagtgca
     1321 tgtatgacct gcaaccacaa caaggctgaa aaaacaccga gagaagctca aatgactcta
     1381 ctgagggcac cattggctcc gaaaccaaat ccatattata ttttctatca taggaaacta
     1441 gaagatgagt ggtctccatt tatcccctgg aaaaattaac ctataaacag gaaaattgtc
     1501 aggttaatga tgtagcgacc ggaagtttta acataaaatt atccaaaacc agactcggag
     1561 taacgttttt ccttaagttg ccgagagtca tcctaataag ttttaaaatg ttcaaaatat
     1621 ctgtggtcga taacatttta gaaaactcgt gaattcgctc tttatgggaa atatttacta
     1681 tatctcctgc tctctcactg ctagagagca acacatccct agtccaggtc attgctatat
     1741 ccaaaaattc atatacccca tcgcggtcac gaaaaaactg agttgataaa tccttcgaca
     1801 atttaaaccg ttgctccaaa ctaccaatta aacaccctac aaactgattc aatttatctt
     1861 ccaatatatg taatatttca gggtttatgg ctgctcgata agcccattct ggtctcccgc
     1921 gcgaaagctt tgctatctct tgaatgggga cttctggtcc taattcacat tcacctaaat
     1981 attccaatat ttctaactca ccaactgggt ccaaattaag aacctggcat cttgacacaa
     2041 tagtactcag aagattcgat gcatcacgcg ccagcaatat aattacaaca tcatctggag
     2101 gttcttctaa cgtttttagc agaatattgg attgttctga tctcatactt tcaatggatg
     2161 aaataatgaa aattcgggtg cggccttcat atggtttccg gtgaacctgt cttaaaaaat
     2221 cttctcttaa ttgatcaatg gtgaccattg tggtggttcg gcctacctca tccaagtctt
     2281 tttttacatc gtaaataaat acatctgtgt gattatggga atcgatacgt atgcactggt
     2341 tgcatgaccc gcaaggcccc gaagatttag gcatttcaag gcaattcgca acttttgcta
     2401 tatcctgcgc aaaggtgacc tttccaatac catccatacc acagattaaa taggcatgtg
     2461 agagtctatc taacacgaca gctctagaaa tacgatctat ggaattaggt tgaccaatag
     2521 ttttccaggc cactttaatc ttccttaacg aaatggattt ccttaaccta aaggtgcaat
     2581 tctacaagcc atttgataat gatccatttt tgaaaatagc acaaactaaa atagctacta
     2641 taatataata tatagtttaa atttaatcac cctcaggttg ctaatcacga tgcagcgaaa
     2701 ttcaattggt aaagaccatc agttaacgat taggatgcta gcaacaggat tgatgctgtt
     2761 ggtagcctat ttagctcttt ttggcttact tctaatttca ggactgaatc ctatttttgt
     2821 atttgtgatt gggattggaa tggttttttt tcaatatttc atgtccgata agcttgttct
     2881 gaaaacaact aaagccaaag ctgttactaa agaagagcaa cccaagttac atgaaatgat
     2941 agaaagactt tctaaaatag gcggaattcc aaaaccacga acagtcgcaa taatggagat
     3001 ggaagcgcct aacgcattcg ctaccggtag gagcccgaag catgccacag tcgcggtcac
     3061 aacaggacta ttgagcaagc taaacgataa agaattagaa ggggtgatcg gtcatgagtt
     3121 agcccatatt aagaatcgcg atgttatggt tatgacctgg gcaagtttga tagtagtggt
     3181 tgctggtttt ttgatgcaaa tgctcttctg gatgagtcta ttcggaggtt ttggaggtca
     3241 taggcgacag ggcaatggag gccaagctat ggccatgatg atggttgttt acattggagt
     3301 aataatcatt tacttcatca gccaattgct tataagtacg ctttccaggt acagggaact
     3361 cgcagccgac agagaaggtg caatcatgac tggtagtccc acaaaccttg catccgctct
     3421 caagaaaatt agtggccaga tagcacaaat tccagaaaaa gatctacgac aagtggaaca
     3481 tgccaacgca tttttcataa tccccgcatt acaaggaaat gcgatcgcat ccttgatgtc
     3541 ctcacaccca cctacagaaa taagagtggc acgattaatg gaaatgcaac agcagaattg
     3601 gcatcttctc aggtagaagg gatcccgtga gattatttag aactcttttt aaaaaaccag
     3661 atagccctga gtccaacgaa tccgatattt tatcaacggt cctgcggatc acaaatcgag
     3721 ataccaatgg gaaaaaagat ataaatctct caggggaatc aacattattg ctaaatgcca
     3781 atgatcatcc ttttttcacc gacataaagt catctctcaa agagttagat aaagaattgt
     3841 tcatgtcgac aatgagaaca ccacttcagg gtttgaaatc tgaaacgtac aacagttgtt
     3901 ggataatatt cgaaagtact aacaatcaaa aaatggtcga tgatataaac gccatcagct
     3961 cccatatagc caatctgggg ttaggagaaa atatgacagc cgcagttttt aaagggacta
     4021 taggagatca atcattttat tgcatatgca actacaggac atcaaaattc tatccattgg
     4081 taccattggg acatgacatg agggacaatg aattagaaat gaaaatcggt gatcggattg
     4141 cctcaatagg gatcccatta gaaccacacg aaaattggta ttccctctct gatgtaccta
     4201 tttagtggct cacatttgtt aaaaaaatag tttatatgct gccacaatcc caaaaggcac
     4261 cataagaatc attaaaaaaa gaaccagtaa agctaaagta gagtccccac caaccaaatg
     4321 ttttactgta tctattaccc agctcatcag atctgggtct cctggttctg aatgatgact
     4381 caaaatcgta aataaaaaag gcatatccaa tccacccaaa cttttatttt agctatagaa
     4441 taaaaagcac tgaaaaaaaa attaattcta ttttattact aattccatga atcgaagatt
     4501 attccagtga atttatgtcg ttactagggg cctcacaact aaatctacac tacgcagata
     4561 tcgaaatttt ttctgatgtt accttgcagg tcaatgaaaa ggcccatatc gggatagtcg
     4621 gtccaaatgg aagtggaaaa acttctctga ttcgggtttt gttggggata caggattacg
     4681 acggtggcga tattttcaag tcagactctc ttcgggtggg ttatgtccca caaacggcat
     4741 tacagtcggg aggtggtact ttgaaagatc aaattggcca ggctttccaa catttaattc
     4801 aattagaaaa agatatggaa accaatgctt tcgccataca agactcagag ggagttgaaa
     4861 gaacacaagc tgaaaagaaa tattcgacgc ttttggataa atacgaagcg cttggtggtt
     4921 atgattacca aaatcgaatg gacagggtcg tagatggagt ggggttaagt gacaccaatc
     4981 tagacacatt agtatcagat gcaagtgggg gtgagagaac acgggctgct ttagcaactg
     5041 ctctattagc agacccagac cttttagttt tagatgagcc aacaaactat ctggacttca
     5101 aaggactcga atggcttgaa gattttttaa acagatcagc aaatgcattt atggttgttt
     5161 cgcacgacag atttttcctc gatcgtgtag cttcggacat ctgggaaata gataattgcc
     5221 gattaaaaac gtaccgtgga aattattcaa aatacaaact ccttaaatct gaagaagatg
     5281 ccaggtttgc taaggagtat atccggcaac aagaatacat aaaaagagag caagatttca
     5341 ttgctcgtta tcatgctggg caaagatcta aagaagctag aggaagagca aaaaagcttg
     5401 gaaggataga aagaatagaa gctccatcca ctgaacaaaa cgtgtccatc acctccgggt
     5461 ttgcaactcg tacagggaaa actgtaatta gcaccaatgg actagaagtc ggtttttcaa
     5521 ccgagcaaca tagcgtcaaa ttatttgaag tcccagacat tgaaatagaa cgaggaacta
     5581 ctacagcaat aattgggaat aatggaatag gtaaaactac cttattaaaa acaattattg
     5641 gaagccttcc agctttgtct ggttcatcaa atctgggaca taacgtaaag gtaggatatt
     5701 tccgtcaagg ctccgacgag atacctagtc atatgacagt gcttgatgca ctgttagaaa
     5761 ttaaaaattt gtctatcggt gaggcaagaa gcttccttgc ccggttcctt ttcactgggg
     5821 aagagattta cgaccaagta tcttccttaa gcgggggaga aagaggaagg ttagctttaa
     5881 gcagactact aataactgaa ccaaatgtac tcgtgttaga cgaacccact acacatctcg
     5941 acatattaag tcgcgaagca cttgaagata tgctgcaggt atttggggga actacaatat
     6001 ttgtatccca tgatcgtcat ctaatatctc tattggctga caacctttta atcatagctg
     6061 agggatctgt ccgacatttc gcagggtctt accaagattt actgaaggag tctaattctg
     6121 acagttcaac aaaagaaaaa gaaaaacctg tccagttgcc tccacccaag aaatcatcgc
     6181 ggtccaagcc tataccaaaa ttaaatggca ccaatccaaa ccgtaacgct gcttctcgaa
     6241 aagaagatca tgaaaagcta atctctgatt tggagtctca aattgaaaat ttggaaaagg
     6301 cacttcaaga ggcttctaac actggcaatt tagataggat aactgtgtta ggacaagaac
     6361 acgaagattt gaatatcaaa ctagacagag ctataagcag atgggttgag tgaaatcggt
     6421 ccaaacagga ttcatgtctt gttgccgaac aactcgtagc cgtggagata ggattcatag
     6481 tcgttcaaat ccaatcgcac ctgatcggtt ttaaaatcaa ttctgtttaa acgagacctg
     6541 tagttctcaa aaacaggttt caatcctaat gtttcatcat taatttctaa caattcatct
     6601 ttcaaagtgt ttgcaaacaa gactggatgt cccccttttc ccttatatct aggcgaactt
     6661 atcggcattc tagttgcctc atgttctttt ataatccgag cgacaatatg accagatgtt
     6721 ggttgatcaa cacctatcaa caaaaaacca cttgaatctt ttggagacga gagaataccc
     6781 tgtcttatag ttgaagcccg gcctgtctca tagtctacgt tacaggttaa ggtgattggt
     6841 gaattttctg gaaactcctt ctgaatctcc tctgatattc tggcacattc ataaccaaga
     6901 acgattacca cgtggccgca gccccctttc agcaaggccc tcacttgatg acaaactaag
     6961 ttggtatctc gccatggtaa tagtgccttg actgtaccca tccttgtgga aaaacctgcg
     7021 gaagtcacta tacccgtaat taacaaattt aatgacctat gtagtctctg ctaaggtctt
     7081 tgaattccga ttcacttttg ctttgattct atcaatctgt tttttatcta gtttcatcgc
     7141 taccccagaa cctcccaatc tgaacataag gatctccgac attatgctaa ttgcaatttc
     7201 ttccggtgtc cgggccctta tatccaatcc aatcggtgat cgtatctcat taaggcgatc
     7261 atattccaag ccttctcgaa taaggtcctc atatatcaat atagtttttc ttttgctccc
     7321 catgagccct acatatttcg cggatgtttt ggcagcagcc aatagggcta cgttatcgta
     7381 cctatgaccg cgagtagcaa ccacaatgaa agtattaggg ttgattggta attgtggcaa
     7441 agcttgttcg ggagtctcag agattattat atcggcctct ggaaaacggt cgtcatttgc
     7501 aaattcttcc ctatcatcag taatgaaaag tctaaactcc aaaggtttcg ccaaagaagc
     7561 cagggcacga gcaacatggc ctccaccaca aataaccagt tgcggcggtg ttgtatatgc
     7621 ctcaatgaaa tactcagttc ccgactttgt agtcacgtac tcatttctac cgtgtatcat
     7681 gagctcaaaa gctttattct tggcttgatc atcttcacta tcatcaccaa tagaaccttc
     7741 actttcgccg ttttcccgaa caaacaattt atcccctatt tttgaataac ctttttttcc
     7801 agttcttacc acggtagcca aagccactgc accaacccca gaatacgcct tatctatctc
     7861 agaggcatag ggaaggtagc tatcaggaga ataaaccggg tctattaaaa aatacatggt
     7921 cccaccacaa actagcccgt cctctgctgc taaatcctcg tttaactcat actctcggta
     7981 ttcagttccc cctcctgcct gcattaactg cttcgcagca aaccaaatgt cgccctccac
     8041 acatcctcct cctagtgttc ctgtccctga accatcttct cgcaccagta attttgcccc
     8101 aggtttttgt ggggtagaac cttttgtccg gacaactgta gccaccacca ctttctctcc
     8161 cctacttaat ccattgatcg catcactaaa tacttcgtgc atctggcctc caggttctat
     8221 agaaaataga tctaaagttt ttcataaatc caccgatgac aaccctatca atcactgata
     8281 ttaagttcaa acttaccgcc attaggaata atacaactat aatacattca agtaacaacg
     8341 tttccagatc aataagcgtg aggaattaat ggtgaacacg gaaacacaaa agatacctct
     8401 attccccctt agtacagtcc ttttcccggg tggtactatt cctttgcaaa tttttgaaga
     8461 taggtaccat tccatggtac aagactgctt ggacactgac tccaaattcg ggattgtact
     8521 tataaaagaa ggtcaggagg tcggaggtaa cgcggttcct cacaaggtcg gaactttagc
     8581 caaaatcaca aaaataagga agctttctca aaaccgtctc tatctcaccg cggttggaga
     8641 aaaacggttc aatatagttg aactaatcca aaataaagct tatctagaag gattggtaga
     8701 aatacaaaat ccacctcccg agcaagacgt agccgagcag tcattgaaga aagctaagac
     8761 tgcagcttcg aggcatttga aggccatcgt atctatgaat ggagggtggg ttaataaagc
     8821 agagactccc ctacctacaa atcccgatca actatccttt tttctggcac aattaattca
     8881 gggtaccaac gaaatccgtc agacaatact tgaggaaaca tccatagaaa ggagaattga
     8941 gatatgccta tttcacctac aagtcgagtc caaaaacata tccagtagtc tcaacacgga
     9001 cctgctattc aaatttagca gaaattagcc cgttgggaat caaactgtta agagtctaac
     9061 gctccttcct tagataagaa atttgccaga cggctgatac cttcagttat ttcgtcaggt
     9121 tggttgtaac caaaacataa ccgtatatga tcctttcctg aagttccatc cggtgagtaa
     9181 ttggtgccgg gatgaaatcc cacgtcaatc tcttcaacag atctcttgtt caaagcaacc
     9241 atatcagaac cttctttcag ctttacccag atatagagtc ccccagcagg atcactccac
     9301 tttgcaccgg taccaaaatt ttcgcctaaa gccgaaagca tagcatctct ttttgtcctc
     9361 tgaatattat tgatttcctc tatatgagca tccaagtatt tccttgcata tttctcgaca
     9421 gccatcgctg taaattcact cacacggcca cttttaaccc ccatggctct atccaaaatt
     9481 aattgcggtg cagtcatgta gcccaaactc ataccaggtg caatggtttt tgagaaagac
     9541 ccgacataca tcacgctgct tgtatcatca agactgagaa tagaggtggg gagatcaccg
     9601 tcgtatctat tatctgcata gcaatcatct tctaaaatag gtaccccata tttttgggtt
     9661 acagtcaata gttctttgcg tctactctca ggtatagtcc atccttgggg gttttgaaaa
     9721 gtagggatta aataaacgaa ttttggcttg tgacccgact tgatagcctt ctcaatttct
     9781 ttatctaatg ccccggggtc cataccactt tcatcagagg caacccccct tatatctgtg
     9841 aaatgtcttc tcaaggtgtt caaagtccca ccatatacga aactatctgt tatgacgacg
     9901 tctccaggat tcaaaagtac cgcacaaatc atgtctatag gttggcctga cccattccct
     9961 aaaatgatgt cattttcagt tacttttatg cctctatctc gctcgatttt ttcagcgacg
    10021 tattgcctga gcggggtatt tccttgtgga tgagggtaaa cagccagttc tgaaccagac
    10081 tctgacaaag cttcttcaag gctttctaaa atttcagcgt gcggcaaaga cacaggatcg
    10141 gggtacgcaa cagcaaaatc atatttccct tttcctgctg gggtaactcg agttgtctcc
    10201 gggacatgtg ttgccaatat attttgaaaa tcaaatgcca tgattcttct cctaatatac
    10261 ctaaaatgct ataactctca atggaaggga atatattcct gcccagagat actagctttt
    10321 taaaccgaac tcttcaacta gttccaatct tctacggcaa tacgtaacct tttgttaatt
    10381 cgccctttgc tttcatgacc cactactctt atttttccta cttccctcgt gttacgaaca
    10441 tgggttccac catctgcctg caaatctagt ccggaaatgt ccactatccg gacttctgaa
    10501 atagcaggag gtagtttatt tattttcatt ctgattaaat cagggatttt aaaggcttct
    10561 tcccgaggaa gattagatat ttttatatct ctttctgcag taacctcttg gttaatgagt
    10621 atttcgatat cggaggcaaa tcctgacgac atttctttaa gttcaaaatc catccgggcc
    10681 tgaagtggag tcatgttgcc tcccgttacc tgggccccaa aatctctcca cacaactcca
    10741 caaagaatat gtaaagctgt gtgtgttctc attagctgat atcgtttaat ccaatcaatc
    10801 ttacctttga cagagtttcc gagatcggga atcggtccat tgactttatg gaccaactta
    10861 ccctcgcttc tggatacttg gtaaatctga gaagattcac cgtcccattc aattactcca
    10921 gtgtcgcaag gctgcccacc tcctccagga taaaaactag tcctgtctaa tacgactcct
    10981 gagtctgtta cgtctgttac ggtaccttca aattcctgca aatagctatc ttcgtagcat
    11041 aaaacttcag tcatgtggac tatataactc ctttatcgat gaggttcctc atttgtaaat
    11101 acaagactcc cagctgccat aaacattcca ccgactccct gacaaaagga ggtttttact
    11161 ccatccactt ggtgagctgc ctctccgcgc acctgcctta cagattcttg aatagcatac
    11221 attccataca taccggaatg agtgtaagaa agtcctccac cattggtatt cataggtagt
    11281 gtgcctcctg gagaagtatt accttcctca ataaaagatc cagcctctcc tctttttaca
    11341 aaacctaaat cttcaagacc ataaataggt aaatgagcaa aggcgtcgta caccattaaa
    11401 tgatctacat catcatgcgt aacacctgct tcctcaaagg cttttttact agaagtctta
    11461 aaagcggccg aagtggtcag atcatacatt tgactgacaa taggactttc aacagattct
    11521 ccagtaccca ttatgtacgc cggtttagta gaaaaatcct tagctctttc agcactagta
    11581 attattaatg ccccgcctcc atcagtgact aaacaacatt caagcagatg aaatggatag
    11641 caaatcatcc tgctattgaa tacatcttca attgtaataa tgtctccgta catggctcga
    11701 ggaactttgt tggcccattt gcgctgtgca acaggaaccg atgctagttg ctcatgcgtt
    11761 aggcctgttt ctttcatgta acgaagcaca cccatgccaa attgagttgt cgggcctgct
    11821 acaccatata ttgattcaaa ctgacctggg aacgagctgt ctcttctgcc tccggacatg
    11881 gtggcaacat ctcctatacg gcccgtctgt tccccgactc tattccgccc gctctcagcc
    11941 atcgccacta atactgtttc gcataaaccg cttgcgatag ctgctgctgc atgacggaca
    12001 tgcactaaaa atgaagtccc tcctacttgg gtgttatcta tccacttagg tactatgcct
    12061 agataatgag ctaaagcagc agggctcatc gtggtggcaa ttccgtctat ctcatttttg
    12121 tctataccgc agtcgttaat agcgttaact gatgcatcaa catgaagttg aaacgctgtt
    12181 ttatccggta tggaacctaa ctctgtagtc tctgaagccc caactatcgc ggctttatat
    12241 cttaatgaat ccatttttac cttcctcttt ctcagttcaa ataatgttgg ctacacctaa
    12301 gctggtttcc acttcggaat cgcaatctca tcattaatgt catcaaacac gacttttaat
    12361 ttcatgtcta gaatgaggtt ttctggggta ttttcaattc ctactatatt ggtcatcatc
    12421 ctagggcctt catcaagctg aacgattgct attgcgtagg gggcatcttc ctcaaatcct
    12481 ggcacagggc ggtgatttat catgtaagag taaagggttg ccaagcctga cgttttgaac
    12541 cactcaagat tatcggacaa acagtcgggg caattcattc tggggtaaaa aaacggcttg
    12601 tcgcagttca aacatctctg tatccaaagc tcatgttgct tcgccttttc ccaaaaaacg
    12661 tccgattctg gtgttggcac cggaattggt ttgttgtaac cactactcat atctttgata
    12721 ctccttttta taaatcttac gaatttaaag gttaaataat ttgtacagaa aacaatattc
    12781 ggattgtatt gacggtaagc attctaggtc aataaacacc ggtaaattaa taaaatttcg
    12841 atctcaaaat caagcttcgc aatgttagaa tcaaactata actcttttga aacaattaat
    12901 ttaagagcat gcgatgtcac ctaaatccgg tcccataaaa aatatagtgg tgaaaggggc
    12961 tagagaacac aatttaaaaa atgtggatgt tactatccct aggaacaagc ttgttgtcat
    13021 tacaggggta tccggttcgg gaaaaagctc cttagctttt gacactatat atgcggaagg
    13081 tcaacgcagg tatgtggagt cattatctgc ctatgcccgt caatttctcg gtcgaatgga
    13141 taagccagac gttgattata ttgaggggct ttcaccggcg atatcaatag accaaaaggg
    13201 aacatcgcat aatcctcgat ctacagtcgg aacggtaacc gaaatatttg attacctccg
    13261 gttattattt gcgagggtag gtaaacctca ttgttacaaa tgcggaagac tggtacaaaa
    13321 acaaaccgtt cagcaaatag tcgactccat cgctggacta cctgtctcca gccggataca
    13381 gatattatct ccggttgtga gaagaagaaa aggggagcac aaggaagtat ttgagtcggc
    13441 aagaaaatct gggtttgtcc gggtgcgtgt tgacggtaaa attagagacc ttagcgataa
    13501 ttttgagtta gacaagcaaa aatggcataa catcgacatc gttgtggacc gacttgtgat
    13561 caccgaaaag atggacatgg taagaataag tgactccact gaggcagctc tgaaacaagc
    13621 tgaaggaatt gtccttgtgg atatttctgg aaaagatgaa atgatatttt cagaagaatt
    13681 ttcttgtgtt tactgcaacg taagccttgg ggagattgaa cctagaacct ttagctttaa
    13741 caatccacat ggggcttgtt ctacatgtac aggcctagga ttcaaaatgg ttgtagatcc
    13801 ggatctagta atacctgaca aaacaaagac tatagaggac ggtgccattg aaggttggtc
    13861 aaaaggagga tcactatctt cctggcacat aagccagtta aattctttgt cagaaataga
    13921 tagtttccga atcgatgttc cgattcagga acttgaacct gaaacaatgg acatgatttt
    13981 gaatggtacc aagaccaaaa ttaaaatgtc ttatcgaagc agaagaggca ggatattccg
    14041 atggaaccgt cgctttgaag gggttatagg aaatttagaa cgtagatata cagagacaga
    14101 atcagaaaag gtgcgtaaag atatagagag gtatatgaca tctcttcctt gccaaggatg
    14161 ttcaggccaa cgtttaaaac cggaatcatt ggcagtgacc ataggcgatg aaacaatcat
    14221 gagtgtgtct gacaaatcca tttcagaagc ggctgattgg attgccttgc ttgaaaaccc
    14281 aaaaattctc tcaaatagag ataagtcaat agcctcacaa attttgaaag aactatctgg
    14341 cagactgcag ttcctactca atgtaggcct cgactaccta acactttcac gcaccgcttc
    14401 cagcttgagt ggcggagaag cccaaagaat acgactcgca acgcagatag ggtccggtct
    14461 aatgggagtc ctatatgttt gcgacgagcc atctattggg ctgcatcctg cagatgatta
    14521 taggctcata aacacactca aaggacttag agatgttggt aacactgtct taatagtgga
    14581 acatgatgag gcaattatgc gagcggccga ccatatagtg gacttggggc caggggctgg
    14641 ggaacacggt ggtgagatcg tcgcatcagg gactgttagc aaaattatga gctccaagaa
    14701 ttcgctaact ggacaatatt tatccggggc aaaagtaatc gacacccctc agaccagaag
    14761 attattcaat ggaaaatatc tagaaatcga aggtgctgaa caaaacaatt taaattcgat
    14821 agatgtgaca attcctcttg gcatattggt ttgtatcacc ggtgtttcag ggtcaggtaa
    14881 aagcacctta attaacgaaa tactctacaa gcgcttgtca caacacttct accgagctaa
    14941 agatcgtcca ggtaaattca aaagcattaa aggtcttgag aacatagaca aggtcgtcaa
    15001 tattgaccaa tcgcccatcg ggagaactcc aaggagtaat cctgctacat acacaggcac
    15061 atttacccct attagagagc tcttcgctca ggtacctgaa gcaagaagta gagggtacaa
    15121 acctggtaga ttttcattta acgtcaaagg aggaaggtgt gaagcttgct ctggggatgg
    15181 ttacataaac atagagatgc aattcctacc tgacgtctct gtaccttgcg aagaatgcca
    15241 agcgagcagg tataacaggg aggcattaga gatagaatac cgaggtttaa gcatctcgaa
    15301 tatcttgcaa tcaactgtaa ccgaagcttt aaccattttt tctaacattc ccagaataaa
    15361 gacaaagtta caaaccctac aggatgttgg gcttggctac atcaaattgg ggcaaccggc
    15421 cacacagtta agtggaggtg aagcccaaag agtcaagtta gcgacagagc tttcaaaaag
    15481 agctactgga cagactttgt acattttgga tgagccgaca acagggcttt cttttgaaga
    15541 ctgttctcaa ttattgagag tgctacagcg gcttgttgac gcagggaatt cagttgtctt
    15601 aattgagcac catctcgatc taataaaaag tgccgattgg ataatagatc ttggcccagg
    15661 tgcgggagat aaagggggaa atttagtata tgaaggaact cctgaaaatg ccacccgaca
    15721 cgaaggttcg tatacaggaa aatatctcaa aaacatactg ccctaaatac atctgtgatg
    15781 gaaagggtaa atcttattct attattggtt atagacagtt aatccttcgg tagtaaaccc
    15841 cttgtaatat aaattgccct aaccgcaaaa agtgaggaat aaatgataaa tatactcggt
    15901 atattgatag ggatacgttg aagattgtta gcctgaaatc catatggtag acttgagccg
    15961 aatttcggct ctattttaaa tgggcaaagt aatgccataa atgaactgta aagcagccga
    16021 acgtaaagct aacaaggggt gctggataaa aaggtgcccc gacaaaggag gaaatctctt
    16081 gaagtggatt gattatgaaa atgcgaaaac ggtggatgag gctataaaaa tcctctccca
    16141 gtcaggcgga aaagctcggc caatggcggg tggaacggac ctgatagttc aattaagaaa
    16201 taaagaccct cgaacggatg cagacgtagt tgtggatata aaggatatac ctgatctcaa
    16261 tgtgctccag tgtgacccta aaacaggact tacgatcggc gctgcagttc ctttgtacaa
    16321 gatatacaat gacgacaacg ttaaaaaata cttccctgca attattgatt cggcatcttt
    16381 gattggaggt gtcctcatac aaggcagagc atcatttggc ggcaatctct gtaatgctgc
    16441 cccaagtggg gatgcgatcc ctaacttaat agctcataac gggattgcaa ccatagcagg
    16501 acccaacgga acacgagatg ttcccgtgga agatatttgc acaggaccta gacaaacatc
    16561 tattgggaat gatgaattgc tgatttctat taacctccct gttcctgcgg caggctttgg
    16621 tgctagctac atcagattca cacccagaaa cgaaatggat atagctgttg taggcgctgg
    16681 ggtctctgtc aatatttcag agggtaagtt cactgcagct cgggtcagtc tggcctccgt
    16741 tgctcctacc cctatctacg tggaaaatgc tgtaaatgct ctcgtcggag aaactgtggg
    16801 agatgctgct attcaaagag cagcaaatgc tgccagagag gcagcaaaac cgatttcaga
    16861 tatgagaggg accgctgagt atagaaaaca tctatgtgaa gtacttacac gtcgagccct
    16921 taatgcggcg atagaacgtg caaaggaagg aaaataatga caacaaaaac acatgtgcaa
    16981 acaacgataa atggtgagcc cgtagacttt ctctgcgagc cacgacagag cctcttagaa
    17041 tgtcttagag atgtggttgg tctaacaggc accaaagaag gttgtaatga cggaaactgt
    17101 ggagcctgca ctgtcaagct ggatggcagg atcgtaactt cctgtttggt cctgggggtt
    17161 gaagctcagg gagccgatat agagaccata gaaggggtag ctgaaggtac gaacctgcac
    17221 cctctacaac tcgccttttt ggaaaatgct gcgctgcaat gcgggatatg tactcccgga
    17281 tttatcgtct cagctaaaag tctgcttgat cagaacccca acccaactga acatgagatt
    17341 cgattttggt tggccaataa tttgtgcaga tgtactgggt acgacaagat tgttagagcc
    17401 gttcaggacg cagcgaaaaa aatgcaggag gcctcgtaga atgacatctt cagacagtac
    17461 ggattataag gttcttggaa ccagacccat taggcacgac ggtgctgata aagttactgg
    17521 acgtgcaata tatggtcccg attttgatac tgctggtttg ctacacggaa aaatccttcg
    17581 gagcccccat gctcatgcga ggattatatc cattgatacc agtgaggcag aggccttaga
    17641 tggggtgatg gctgtagtta ctggtgccga ttggcctaat aatagtaatg gaacgagcat
    17701 ggttgaagac gggactggta ccgaaattga atcaaattcc aactggctcc gtgaaaatct
    17761 attagccgga gataaggttc tttacgcggg ccatgcaatt gctggagttg ctgcggcaag
    17821 tcctcatgtt gcggaagaag cccttgcgct catagaggta aaatacgaag ttctggagcc
    17881 cgttttaaca atccaggaag ccatggcaaa aaacgcacct atattgcatg aaggtttgac
    17941 aacccaagaa ttgggagagg attccggcgt tgttagtaac actgcggatc attttcagca
    18001 taccaaaggt aatattgaaa aaggtttcga ggctgccgat gtagtggtag agcgagaata
    18061 caatacggtg atggtgcacc aaggttatat tgaaccccac aatggaactg ccctatggaa
    18121 tacagatgga aggctccatg tgtggtgcag tacccaagga tcatttgtag tcagagatag
    18181 cctctcagac cttttggata ttcctgtttc ccaaatacgt gtaacaccca cggaaatagg
    18241 tggtggattt ggaggaaaaa tccctatata tgtagaaccg gttgcagctc ttttatcaaa
    18301 gaaaacaggg tctcctgtca agatagtcat gagccgtaag gaagtctttg aaggatctgg
    18361 accgacagca ggatctaata tcaaaataaa acttggcgcg aaatcagatg ggacaattac
    18421 cgcggcgcat gcttggatgg ccatggaagc cggggggtat cctggctccc ctgtaggggc
    18481 agcagcgcaa tgtgtatttg ccgcttacga tatcgaaaat gcacttgtcg acggatatga
    18541 tgtcgtagtc aacaaaccca aaaccgcccc atacagagca cccggtgcgc caaacgccgc
    18601 atttggaatg gaacaagcca tgaatgaact tgctgaaatg cttcaaatgg atccgatcga
    18661 cttgcgtctt aaaaacgtag caaccgaggg aactagaaga gttgatggcc ctacctttag
    18721 gcgaattgga gcaaaagagg tacttgaaac catgagggat catccgcact atcaagcggc
    18781 taaaactgaa ggtcacggaa gaggaattgc gattggattt tggtttaata tcggatttga
    18841 ttcagcctgt aacatcgcag taaattctga tgggtcgatc aaccttacgg aggggtccac
    18901 cgacattggt ggttctagaa catccattgc aatgcacgca gctgaagttt tgggaattcc
    18961 ggtggaagat gtcagaccgt cagtggtcga tacagacact attggcttca cggcggtaac
    19021 aggtggaagt agaactactt tcgctaccgg gtgggcagca tatgaggctg cccaagatgt
    19081 gaaaaagcaa tctatcgaaa gggcagcaag catttgggat gttgattcag acaccctaaa
    19141 tatggaagat ggtgtcatca aatcgaaaac agattctgag ctaaaaatga ctttcaagga
    19201 actagcatct caaatgagtg acaccggtgg aggaataact ggaagagcgt ccgtcaatcc
    19261 taaaggagtt ggggggtctt tcgccggcaa catggttgat gtttctgttg atgacgaaac
    19321 cggaaaggtg actattgaaa gatttacctg tgtacaagac gtaggaaaag ctatccatcc
    19381 cagttatgtg gaaggccaaa tgcaaggtgg tagcgcccaa ggaatcggat gggctctcaa
    19441 tgaagaatat ttcatgaacg accaaggtaa tatggcaaac tcaagtttgc tcgattacag
    19501 aatgcctact tcattggatt tacccatgat tgatacagcc atagttgaag tagcgaatcc
    19561 aggacatcca ttcggggtaa gaggagttgg agaggcgaat atagtacctc cgaccccagc
    19621 tattgcaaat gcagtatctg acgccactgg taatatcatg acacaacttc cactgactcc
    19681 aggtgccgtg cttgattctg gaaagtaatt aatgcctata attttcgtgc cttctcttat
    19741 gcagaagcta tgtaatggtg aacaaaagct gctaattgaa ggtacgaact taagacaagt
    19801 catagataat ctagacctac aatacacagg atttaaagat agattggtgg aagacggaaa
    19861 aataaagcca aatatatccg tggcggtaga tggggaaata acaccattag gtatgattca
    19921 gaaggttgac actaatagtg agattcattt cctaccggcc atttctggcg gataaaacat
    19981 aaaattttac aactagtccc gataaagtca gggctatttt tattgcgtga aatgaaacca
    20041 aaccaaagag gccctaaaag ccgatttcta gacaataaaa ccaatttaac ccattacatt
    20101 gattggcaag gtgaagaaca aaccaccctt ttaattcatg gtgacatgag aaccagtagg
    20161 agctttgacg cagtcgccaa gaatctatcc aaagacagcc atgtattggc catggacctg
    20221 ctcggccatg gagacagtac ttggctagac agaggatatc ggttctctga taggtccgat
    20281 gacatagaaa atttcatcac tggtaccaac ttaagcaagg tacatgctgt ggcacattca
    20341 accggtgctg tagctttggc catgtatgtc gccaatagtc ctgaaagatt cgagaaattg
    20401 gtgttactgg aaccaatgat gattgtcgat aggaaatttc aacgaatggt atctgatagg
    20461 ggcaatagac caagaactac atggaaggat cacgatgaac taaaagatct tttgggcaag
    20521 catgaagtca caagcaaatg gcatccagac gtgatagagg atgtcgttaa tcatgagaca
    20581 tttttgaatg aagacggcag gttggatatg aaatggtcat cttctaccct agcatgggaa
    20641 gaaagagaaa acgactacct agatttagaa ccgatcctaa aatcgatatc actacccata
    20701 ctatttgtgg tgggggaaac ccgtatagga gggtttgaaa aagcttttca gctggaaagc
    20761 agcctacaaa acatgcaaac tgtcgtcata agtaacacag ggcacaatat gtacatggaa
    20821 cgcccagagg ctgttgcaga agtcattcgg aaattcagaa attcctctcg tctaccttca
    20881 agggtttaga gatcatatct tttctatctt taaaagctag gcagtccaaa cgtccattct
    20941 gagggtgatg cagctggaga accataactg cctttggggc ctttccacgg gtgagccctt
    21001 gcgacctcct cgttaatgtc ggtaccccaa cctggtgctg atggtattgt catataccca
    21061 tctatgattt caggtttgtt ggtcaccaaa tcgtctttcc agggaacatc atcaatgtca
    21121 atttccatta ttctgatgtt tggaagggag gcgcatagtg atgcactaac aaaggtagac
    21181 aaatgactat aatagttatg aggtgcaaca ttatgctgaa atacttccgc taaatcacct
    21241 atcttcttag actgactgaa accgttccaa ggcacatcaa tcatgaacat atcagcagcg
    21301 tgctgctgaa agtaaggaag gtaatctcgc atataaaata aattttctcc agtacaaatc
    21361 ctagtggttg tagaatcctt tatttgcctt atactctccg gctgatacat gtctatttcc
    21421 agccacagca tatcgaactg ctccaagact tgggcaatcc gcaggcaagc ctcggtttta
    21481 aagtggaaat tgagatccaa attaatatct acatcgggcc caactgcatc tctaaatgtg
    21541 ccaattagct tctcaacgtg cctcaaaacc cccttagtaa ccacttggtc agttgatcct
    21601 cttcctccac cgaatccacc gccgaaatgc tgagccggat cacctggtcc aataatattg
    21661 gtctttagag cagtaaagcc gcggtctact acttcctttc caagagcggt tacatcatcc
    21721 catgtcctaa gtggcgggac accaattaat tcataattat ttgttcttga agtaccgcaa
    21781 tgagaccagt agacacgaac tttgtcgcga gtcggccctc caaaaagttc agggactgaa
    21841 atgcctaaac tttttgcttt gatatcaata aaagcgcaat ccaaccctgc caatgctttt
    21901 gcagcaatgc cccccgggct ttgccgggta cctctataca tatcccaaaa ccgattttca
    21961 aattccctag ggtctgtacc tataagcagt ggcttcaaat cctcgatagt accctgtaca
    22021 ccgcgagggg atctgccatc gctacactct ccccatccag taatgccttc atcggtttca
    22081 accttgacaa aaccccaagg tctccaacct gcatcgacta tgaaggtttc tatatttgtg
    22141 attttcacct taaaatatcc tcactttccg ctaattttcc agcgggtaac tgaattcttc
    22201 ccttaaaact ctcttaagaa ctttgcctag aggatttcgg ggcaactctt ctaccaccac
    22261 aactgattct ggcttcttga aactagccat cctatccttg caataagcca tcacctcttc
    22321 ttcatctata gtggcaccta tctttgctac aactattgcc ctaacacgtt ctccccaatc
    22381 tatatccgga actccaataa tagctgcttc atcgatggag gggtgtgaat gtaatacttg
    22441 ttccacctct tctggggaaa ccatttcacc agctctctta ataaaatctt tagccctacc
    22501 tgaaagaaat atatatccat cctcatcgaa gtatcccaaa tcaccagtgt acagccaacc
    22561 tcctcttagc gcctctgccg ttgcttcttg ttggttccag taccccttca ttaacctagg
    22621 gccctgagcg actatttctc cagtctcacc cacattcaca tcacctcctt catcatttac
    22681 gatccttaca tccacatcac tcaaaggttt ccctattgat gttaatctat ttaatttttt
    22741 ttcgatttct tcttctgaac cttccaaaac atggtcttct ggcggcagca tagttatggt
    22801 tgctgctgtc tcggtttgac cgaaagcatt tatgaaaaag gttcctggaa attttgatat
    22861 agctcttttt atcacttcaa gcggcatcgg ggctgctccg taagtgatca cctctaacgt
    22921 tgaaaggtca aatttttcaa attcctggtg atccatcagc attttaagca tcgtcggaac
    22981 catcatggct cgattagctc tttcttcttg taccaatgcc atccattcaa ggggttcaaa
    23041 ttgccgttga atgattaaag tgcgacctcc atatatcgca gccatgacag cctgtatacc
    23101 ggcgatatgg tataagggga ctgtcaggat gtttttctcc tccgattcga gttctggagg
    23161 ggaaacattt gccaatatat acgacgcgaa actgtcatga ctcagcatga cccctttggg
    23221 agaacctgta gtccctgctg taaacatgac catagtcaaa tcgtcttcgt cacccgtagg
    23281 gtaaaggtct tcgtcagatg acaccgacag caaatgttca tatgattccc acccttcctc
    23341 agatgcatca tcaagcacta cgtatccggc gacactagtc aagtccggtt tgatttcatc
    23401 taccatagaa acgtaacgtt caccggtgat gagaacctta ggcttggaat catttatcat
    23461 atattctatt tcttctgggc gtgaacgaaa attaagcggt acgtacacag catctaactt
    23521 ggctgctgca aagtaggtct ctatattttc gttacaattg acctgcatgg tggctatcct
    23581 atctcccgct cctaccccca tgcctgaaag ggcatttgcc aaccttttta ctctttcttc
    23641 caactcttga aaagaaaacc ttttaccgtc aaaaactata gccgtcctgt ctggaacaat
    23701 tagagaggtg atatttaaaa attctgtggt gttcatgaat cgtgtattac tcctaggccc
    23761 ttggaatcca agaggtaatt taacaccatg gaatactaat atccaaatct atctaggtaa
    23821 ttagctataa ggaagcatag aaatcaagat aatttcaacg attttgttaa tagtcaccaa
    23881 cctctgatag aattacaagt ccgttaacat cagctaactt ttagattagt gtcttaaaaa
    23941 agggagattt ccttggcctc aaacaatgaa atgaacctgc ttgaggcagt cggtgaatac
    24001 accggaagcc ttaaagagaa agataagtct caggcagcca taagagagat aaatcgcttc
    24061 gttaaacatt ttcaagggga acgtctcctg gcatctataa caccatcaca gataggtggc
    24121 tatgctgagc aagttgcaaa aaacacgatg tctgaggaag cagtagaagg actgcaggct
    24181 gttcggaagt tcctagctta tatccacaaa caagaaataa cagatatgaa cctagccact
    24241 cacttcaggg ttagaaaatc aaaaggcaac tctaggacag gaagaggaag atctgtgtcg
    24301 caaaggggcg ggcaagaaat gactcaggaa gggcacgacc aacttacatc tgagaaggat
    24361 ctgcttgaat cgaatagggt atccatcagt gaatccatac aaactgcggc tgaagatggt
    24421 gatgttaggg aaaatgctcc tcttgaagca gcacgggagc agcagggaag ggaggaagcc
    24481 cgtatcaagg aaattgaagg catgctcaga tccgcaataa ttgttgattc ttcaggcaag
    24541 ggggcaaaaa cagttcgggt tgggaccact atagtaattg aagatgtcga caaaaaaaag
    24601 aagagcaaat acactctcgt cagcccttca gaggctaatc ctcttgaagg caaaatatcc
    24661 gatgcctcac cgttaggaaa ggcattgata ggaaaaaggt ccggtcaaaa ggctgtggca
    24721 gtcactccaa aaggtcaagc gaacctcaag atacttgata taaactagcg atccttggta
    24781 taaactaaat cgctggtaag ttttgaggcc ggagtcacaa acccatatat taatgcaggt
    24841 tccttcacat ggtaattact tccaaagctt ctgacacgca agagcttgta gctgagttac
    24901 agaaacatgt ttcgggagag gtgcgtttcg acaaaatgtc gcgcgcccta tggagcaccg
    24961 atgccagcat ctatcagatt gaacctgtgg gcgttgtttt acctcgcaat gacgaagacg
    25021 tcatagcggt tattgaaaca gctcacaagt acggtgtcac ggtgttacct agaggcggcg
    25081 gtaccagcct cgctgggcaa actgtcgggg aatcagtggt agtagatttc tcaagatata
    25141 tgaagagtat gggagaagta aatgtggagg aaggatgggt aaaaacccag cctggaataa
    25201 ttcttgatga attaaatgct cagctgtcac ctcatggggt tatgttcaca cctgatccta
    25261 gcaccagtag tcggggaaat gtcggtggag cactgggtaa taattcgtgt ggcgcgcatt
    25321 caattctatg gggtaaaacc gtagataacg tctacgatct taacgtggtt ctgtcaaatg
    25381 gcgacagaac aaaattcgaa catttaaccg gagatacctt ag
//
LOCUS       DASCNS010000106         1134 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000923203, whole genome shotgun sequence.
ACCESSION   DASCNS010000106 DASCNS010000000
VERSION     DASCNS010000106.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000923203"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="QF838_03895"
     CDS             <1..587
                     /locus_tag="QF838_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59865.1"
                     /translation="KISIDYFPPGEVITATNASSKFKDLSNTTLRGQLIDFVSESSTE
                     ASPHRIKMWSYAGQVSANWGGYQILLTIEGIPAADPSTVACVADFTIKNSGGTAFTAT
                     QMEEKLDVFVVLNTAAGDTEMKVPVKGSAPLQSNQWTYSISDDSYQVGVFNPGETITM
                     KINLNSEPQVAGTLLVTLPNGVGMSHSFPSICTM"
     gene            673..>1134
                     /locus_tag="QF838_03900"
     CDS             673..>1134
                     /locus_tag="QF838_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HJO59866.1"
                     /translation="MTTEEASVITTGSTEIDRRLGGGIPYNTVMLIEGQDASGKSTFA
                     QQLLWGTLNSGHKATIYTTEQNVHALLRQMESLGQDATDYFLLNDLQIFPLTSTAGSE
                     DPDLFFKLLSDHIRQQDSSRMIVIDSLTTFVSRAGGEQIQDFFNEMKNVCEK"
BASE COUNT          359 a          223 c          243 g          309 t
ORIGIN      
        1 ccaagatttc cattgattat tttccacctg gtgaggtgat tacggcgact aatgcatctt
       61 ctaaattcaa ggatttatct aacacgactt tacggggaca attaattgat tttgtatctg
      121 aatcatctac ggaggcctcc cctcacagga taaaaatgtg gagctatgcc ggacaagttt
      181 cggctaattg gggaggatat caaattttat taacaataga agggattcca gcagcggacc
      241 cgtctactgt cgcttgtgtt gctgatttca caataaaaaa ctctggtgga accgctttta
      301 ctgcaactca aatggaagag aaattggatg tcttcgttgt actgaataca gcagccggcg
      361 acactgaaat gaaggtacca gtcaaaggaa gtgcccctct acaatccaac caatggacct
      421 actccataag cgatgatagt tatcaggttg gagtattcaa cccaggtgag accatcacca
      481 tgaagataaa tctaaacagt gaacctcagg ttgctggaac attgctggtc acattaccaa
      541 atggagtagg tatgtcacac tcttttccct ctatctgcac catgtaacaa caatgccaaa
      601 cattgtagta ttgtgagaaa attaataggt cttgattctg taaaaaatat ggaagaattc
      661 tagggcggtc aaatgactac agaagaagca tctgtcataa ctacagggag tacggaaata
      721 gataggagac tgggtggagg tatcccctac aacacggtca tgcttattga aggtcaagac
      781 gcttctggta aaagcacctt tgcacaacag ttgctttggg gcactttgaa cagtggccat
      841 aaagctacga tatataccac cgaacaaaac gtacacgcac tattaagaca aatggaaagc
      901 ttaggccagg atgcgacaga ctattttttg ctaaatgatt tacaaatttt tcctttaacc
      961 tcaacggctg gcagtgaaga tccggattta tttttcaaat tactttctga ccatattagg
     1021 cagcaagatt catccagaat gatagttatt gattctctta caacatttgt ttcgagggcc
     1081 gggggagagc agatacagga tttttttaac gaaatgaaaa atgtctgtga gaaa
//
LOCUS       DASCNS010000107         1408 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000931121, whole genome shotgun sequence.
ACCESSION   DASCNS010000107 DASCNS010000000
VERSION     DASCNS010000107.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000931121"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="QF838_03905"
     CDS             <1..291
                     /locus_tag="QF838_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59867.1"
                     /translation="SGSSAVTSASSADDDLGSKCAQVSAAWTLASELKIDQPDLTFIG
                     LQQKDWVGPNLGCTESSEGNQEINPGFEVEFSYSGSNYLVRSNKYGSLVKIC"
     gene            372..869
                     /gene="ybeY"
                     /locus_tag="QF838_03910"
     CDS             372..869
                     /gene="ybeY"
                     /locus_tag="QF838_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HJO59868.1"
                     /translation="MEESIVLNCHYDDIFDIEHFTINLIKAGKNTLRKCGISSGEASI
                     YITGDEYLKILNEKYRGIDEVTDVLSFSNDHEGEYYGAEEDNRVGLSHLEFITPDDLT
                     KQIGEVVISFPQAERQALEHDLELQEELKFLVIHGFLHLLGYDHIGVDDRNEMESLEK
                     EIMAG"
     gene            880..>1408
                     /gene="dnaX"
                     /locus_tag="QF838_03915"
     CDS             880..>1408
                     /gene="dnaX"
                     /locus_tag="QF838_03915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HJO59869.1"
                     /translation="MAEALYRKWRPGTLSELVGQKHVAQTLQQAVLTDRVSHAYMFCG
                     PRGTGKTSTARILAKAVNCLSPSEGEPDDTCHMCLAVNEGRAIDLIEIDAASNRRIAD
                     IRDLTEKIHYSPAEAKFKVYIIDEVHMLTTEAFNALLKTLEEPPPHAILILATTDIHK
                     VPLTIISRCQRFDFRR"
BASE COUNT          437 a          255 c          320 g          396 t
ORIGIN      
        1 tctggttctt ccgctgttac gtctgcttct tccgcggacg atgatcttgg ctcgaaatgt
       61 gcccaagttt cagccgcatg gacactagct tctgaattga aaattgatca accagattta
      121 acttttattg gtctccaaca aaaagattgg gtaggaccta acctgggctg cactgaatct
      181 tcagaaggga atcaggagat aaatcctgga tttgaagttg aattttcata tagtggttca
      241 aattatttag tgagatctaa caagtacggg tcattggtca aaatctgcta ggtgaattag
      301 tacctaaggt gcatttgcac tcaaatagca ctaattgagt ctgaataggt tcacttttag
      361 gtgtgagtca attggaagaa tcaatcgtat taaattgtca ttatgacgat attttcgata
      421 tcgaacactt cacaataaat ctcattaaag ccggaaaaaa cacactgcga aaatgtggaa
      481 tttcatctgg tgaggcgagc atatatatta ccggtgatga gtatttaaaa atactaaatg
      541 aaaaatatag gggaattgat gaggtaactg atgttctgtc cttctcaaat gatcacgaag
      601 gagagtatta tggggctgaa gaggataacc gagtggggct gtctcactta gaatttataa
      661 ctcctgatga cctaaccaaa caaatcggag aagtcgtgat atcttttcct caagctgaaa
      721 gacaagcgtt agagcatgat ttggagttgc aggaagaatt gaaattcctg gtgattcatg
      781 ggtttttaca cctgcttggc tatgatcata taggggttga tgacagaaat gaaatggaat
      841 cgttggaaaa ggaaataatg gctgggtgaa tatatagata tggctgaggc actgtacaga
      901 aaatggagac ccggtacatt atccgagtta gtaggccaaa aacatgtggc ccaaactctc
      961 cagcaggctg tcttaaccga tagggtatcg catgcctaca tgttttgcgg gcctcgagga
     1021 accggaaaga caagcacggc taggatattg gccaaagcgg taaattgcct atcgccaagc
     1081 gaaggggaac ccgatgatac ctgccatatg tgccttgcag tgaatgaagg tagagctatt
     1141 gatcttattg agatcgatgc agctagtaat cgtcgtatag ctgacataag ggatttaact
     1201 gaaaaaatac attacagccc tgctgaagct aagtttaaag tttatattat cgatgaagtt
     1261 cacatgctca caacagaagc ctttaatgct cttttaaaga ccttagaaga acctcctccc
     1321 catgccatac taattctggc tactactgat attcataagg tcccattgac cattatatct
     1381 agatgccagc gctttgattt tcgaagag
//
LOCUS       DASCNS010000108         2421 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000938520, whole genome shotgun sequence.
ACCESSION   DASCNS010000108 DASCNS010000000
VERSION     DASCNS010000108.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000938520"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="QF838_03920"
     CDS             complement(<1..914)
                     /locus_tag="QF838_03920"
                     /inference="COORDINATES: protein motif:HMM:NF018822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HJO59870.1"
                     /translation="MNKLLFSLMALSSFLLFACGSDSEIDSTATVEIENTAIVETIST
                     ATPVITQPPPITPTATQPITTQIVVPTKVPQESITQDNKIGSVEPDSRSVVEIISRLK
                     EELKNGYSHHSILNMNMTISFDDYLEDMPLLMEGDVQNSRNFKGVTSFEDNNQIKMIE
                     MIVFDQDTFAKYPDTKYWIQFPEGQTPVTPISIIELLKDNFVQGKLTGIEKSGGVSSQ
                     HISSELEAKIFGELLPVLDDSTGILQVDMWVEPDSSQIGRVLINGLAIGGPRVKGPEG
                     QEVTIKVDIEMSSWDFGKVVELSKPDLPG"
     gene            1019..2047
                     /locus_tag="QF838_03925"
     CDS             1019..2047
                     /locus_tag="QF838_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59871.1"
                     /translation="MGSVIQSIVGTSEKGYSGDGGLAKEATMDNPFHVDIDPTGKYLY
                     IADCFNFRVRRLNLDSGIIENFAGTGLQGHTGDGGYAKQASIDEIYAIQVDKIGNVFI
                     CQRFNPSIRVIDKSTNKIRTIVGTGKPGSGEDGISATKSAMIEPNDCAINNDGHLLIA
                     DVQDQRIRKFDTNTGIISTVAGTRVKEHTGDGGPAIHAGIFGARAVCTDKIGNIYICE
                     REGNTIRKIDTDGIISTIAGTGERGYTGDGEDAKYALLNGPKAIRCDSDGNILVVDTE
                     NHALRIIYRENNIINTIAGGTLGSGGDGGPSTMAGLARPHGVVTDLSGITYIADSENH
                     RIRTICYQ"
     gene            2057..>2421
                     /locus_tag="QF838_03930"
     CDS             2057..>2421
                     /locus_tag="QF838_03930"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HJO59872.1"
                     /translation="MLNSFYGMHWMLATPFDQKEEVDYESIPNLVAEAISSGCTGVVG
                     LGVMGEAARLSDEERTKIAETIIDAADNLPVTLGTTANGTKAMIERSKEAEQMGAAAV
                     MVSAPSMPKANLDALFGYY"
BASE COUNT          776 a          469 c          526 g          650 t
ORIGIN      
        1 cctggtaaat ctggtttaga taactcaacc acttttccaa aatcccatga tgacatttca
       61 atatcaacct taatcgttac ctcttgtcct tcaggacctt taactctagg tcctcctata
      121 gccaatccat taattaatac tctacctatt tgcgagctat caggttcaac ccacatatct
      181 acttgcaaaa tacctgtgga gtcgtccagt actggcagca actcaccaaa aatctttgcc
      241 tcaagttcac tgctaatatg ttgagaggag acaccaccag atttttcaat accagtaagt
      301 ttcccttgaa caaaattgtc ttttagcaat tcaattatcg atatgggggt tacaggggtt
      361 tggccttctg gaaactggat ccaatatttg gtatcgggat atttggcaaa agtatcttga
      421 tcaaagacga tcatttcgat cattttgatt tgattattat cttcaaaaga ggtgacccct
      481 ttaaaattac gagaattttg aacatctccc tccattagca atggcatatc ttccaaataa
      541 tcgtcaaatg aaatcgtcat attcatattc aaaattgaat ggtgtgaata cccatttttt
      601 agttcttctt tcagacggga aatgatttca accactgatc ttgaatccgg ctctacagaa
      661 ccaattttat tatcttgggt tatggattct tgaggcacct ttgttggtac aacaatttga
      721 gtagtaatcg gttgggtagc tgtcggtgtg atggggggtg gttgggtaat tacaggtgtg
      781 gcagtagaga tcgtctcaac aatggcggta ttttcaattt ctactgttgc agttgaatct
      841 atttctgaat cggacccaca agcaaataac aagaaggacg ataaagccat caaagaaaat
      901 aataatttat tcatacactt gctacttata ttaatattta cacattcttc ctataatatt
      961 ttaccggtga tatagaatga tttgtgaaaa aaaatagcga atattcaggg aataaaattt
     1021 gggctctgta atacaaagca tagttggcac cagtgagaaa ggatatagtg gtgacggagg
     1081 cctcgccaaa gaggccacaa tggataatcc attccatgta gacattgatc cgactggaaa
     1141 atatctatat atcgccgatt gttttaattt ccgggtgcga cgcttaaatt tggattcagg
     1201 catcatcgaa aattttgccg ggactggtct gcagggccac accggtgatg gaggatatgc
     1261 caaacaagcc tcgatagatg aaatatatgc aatacaagta gacaaaatag gaaacgtttt
     1321 catatgccaa agattcaacc catctataag ggtgatcgat aaatctacta ataaaataag
     1381 aactatcgtt ggtacgggta aaccaggttc tggagaggat gggatttcag caactaaatc
     1441 agcaatgata gagcctaatg actgtgccat aaacaatgac ggccacttat tgatagcaga
     1501 tgtgcaagac caaagaatac gaaaatttga taccaatact gggatcattt cgaccgtagc
     1561 tgggaccagg gtgaaagaac acacagggga tggaggcccg gcaatacatg ctggaatatt
     1621 tggtgccagg gcagtgtgca ctgataaaat tggcaatata tacatctgtg aaagagaagg
     1681 aaatacaatc cgaaaaatag acacagacgg aataatcagc actattgctg gaacaggaga
     1741 aagaggctac accggtgacg gcgaagatgc aaaatatgcc ttgctcaatg gtcccaaagc
     1801 cataaggtgt gacagcgatg gaaatatatt ggtggtagac acagaaaatc acgctctccg
     1861 aatcatttat agagaaaata acattatcaa caccatcgcg gggggaactt taggatcagg
     1921 gggagatggc ggtccttcta ccatggctgg gctcgcccgg cctcatggtg tggtaacgga
     1981 cctctcagga ataacttaca tagctgacag cgagaatcat cgtataagaa caatttgcta
     2041 ccaataggag acccatatgc ttaactcttt ctacggaatg cattggatgt tagcaacccc
     2101 cttcgaccaa aaagaagagg tggactatga atcaatacct aatttagttg ccgaggcaat
     2161 atcctctggt tgcacgggag tcgttggttt gggagtgatg ggtgaagctg cacgtctatc
     2221 cgatgaggaa cgaacaaaga ttgcagaaac aattatagat gcagccgaca atctacctgt
     2281 aactttagga accacggcca atgggacaaa agcaatgata gaaagaagta aagaggctga
     2341 acaaatgggt gcagcagcgg tcatggtttc agcaccttca atgccaaagg ccaacttaga
     2401 tgccttattc ggatattact a
//
LOCUS       DASCNS010000109         1616 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000949389, whole genome shotgun sequence.
ACCESSION   DASCNS010000109 DASCNS010000000
VERSION     DASCNS010000109.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000949389"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="QF838_03935"
     CDS             <1..981
                     /locus_tag="QF838_03935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO59873.1"
                     /translation="GNVLPIWDDPLWLVEQLATIDMISKGRLVSGWVRGTARESVAHN
                     SPPAYNWERFQEAHEFIIKAWTEPGPFRWEGKHYQYRYVNPWVRPYQKPHPPIMLPGV
                     VSRDSVMWAARQRIPYIMLATQLEPTRQSFEVYKETAREEGYEAGPQNFGYLWKVHVD
                     ETEELAEEVARKFVQGPSNPFNAGNEGTANPALNKLPGMTSRRRTLPTAQVATANRGG
                     QLSRSFEDQVSDYSIISGTPKTVIPKIRHVLEYLRPGSVFFWDGDGAMTHDDQMRSLR
                     LMGEEVLPAVREIADELGLDSPFEVDPKTGKNFDAIESESPDEIAATPGD"
     gene            complement(1045..>1616)
                     /locus_tag="QF838_03940"
     CDS             complement(1045..>1616)
                     /locus_tag="QF838_03940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO59874.1"
                     /translation="VSIQPLDVSDNDAVLSVADSILNEYGQIDIGVFSAGINVRERNW
                     PVVSIEDWDKVINIDLNGAFYCCQAVLPSMRSNGGGLIINVSSMAAKSAGALTGPAYT
                     SAKHAMNAMTASLIVEERNNGIRATALCPGEVATPILEQRPVPVSAEDQARILQSEDL
                     GELILFLAKQPPHVTINEVLINPTWNRFA"
BASE COUNT          457 a          347 c          382 g          430 t
ORIGIN      
        1 ggtaatgttc ttcctatatg ggatgatcca ctttggttgg ttgaacaact agcaaccatc
       61 gacatgattt ccaagggtcg gttggtttcg ggatgggttc ggggtactgc tagagagagt
      121 gttgctcaca attcacctcc tgcatataac tgggaaagat ttcaagaggc ccatgaattt
      181 atcattaagg catggactga accgggaccc ttcagatggg aagggaaaca ctatcaatac
      241 cgatatgtta acccgtgggt gaggccatac cagaaaccac atccccccat aatgcttcca
      301 ggtgttgtta gtagagattc ggtcatgtgg gctgctaggc aacgaatccc ctacataatg
      361 cttgctacac aattagaacc gacaaggcaa tcttttgaag tttataaaga gacagctaga
      421 gaagaaggtt atgaagcagg ccctcagaat tttggatacc tgtggaaagt tcacgtggat
      481 gaaactgagg aacttgcaga agaagtcgcg agaaaatttg tgcaaggtcc tagtaacccg
      541 ttcaacgcgg ggaacgaagg tactgccaat ccggctctaa acaaacttcc aggaatgact
      601 tcacgtagaa ggacacttcc aactgcacaa gttgctactg ctaacagagg agggcagtta
      661 tctaggtcct ttgaggatca agtgtcagac tattcaataa tatctggtac tcccaagact
      721 gttataccga aaatacgtca cgtacttgaa tatcttaggc caggctcagt gtttttctgg
      781 gacggtgatg gagctatgac tcatgacgat caaatgagaa gtcttaggct tatgggggag
      841 gaagtattac cagctgtgag agaaatagca gatgagcttg ggctagatag ccctttcgag
      901 gttgatccca agacaggaaa aaatttcgat gcaatagagt ctgagagccc cgacgaaatt
      961 gccgccactc ctggagatta aagcaaataa tataaatccc tctgagatca ttaaaatcct
     1021 cagagggatt tatacatttt tagcttatgc gaaccggttc caggtggggt taattaacac
     1081 ttcatttatt gtcacgtgtg gtggttgctt tgcgagaaac aatatgagct ccccaaggtc
     1141 ttctgattga agtattctgg cctgatcctc ggcactcacg ggtacgggac gttgttccaa
     1201 gatcggtgta gctacttccc ccgggcacaa tgctgtagct cggattccgt tgtttctttc
     1261 ctcaacaatt aaactggcag tcattgcgtt cattgcatgc ttcgcagaag tgtaagccgg
     1321 tcctgttaag gctcctgcac ttttagcggc catggaagaa acattgataa taagcccgcc
     1381 tccgttgctt ctcatagaag ggagtacagc ttgacaacag tagaaagctc cattcaagtc
     1441 tatatttatg actttatccc aatcttcaat tgagactacc ggccaattgc gttccctgac
     1501 attgatacct gcactgaata caccaatgtc tatttgacca tattcgttta atatcgagtc
     1561 ggcgactgat aaaacagcat cattgtctga aacgtctaga ggctgtatcg aaacat
//
LOCUS       DASCNS010000110         1552 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000960409, whole genome shotgun sequence.
ACCESSION   DASCNS010000110 DASCNS010000000
VERSION     DASCNS010000110.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000960409"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="QF838_03945"
     CDS             <1..306
                     /locus_tag="QF838_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO59875.1"
                     /translation="TDYPPGPYEPLLGIQSCVTRTDINGTLWGPSQKISAKDALRIYT
                     LNGAYASFEESHKGSIEPGKLADFVVLEEDILDVDPFSIKDIKVIKTVVGGKPTYEI"
     gene            complement(303..>1552)
                     /locus_tag="QF838_03950"
     CDS             complement(303..>1552)
                     /locus_tag="QF838_03950"
                     /inference="COORDINATES: protein motif:HMM:NF039585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HJO59876.1"
                     /translation="ADEVRRQFLIPLWNVYSFFVTYANIDKFDPEAQSPNVSDRAELD
                     RWILSELNILVQTVTDNLENFQPERVTREVEEFIEYLSNWYVRRSRRRFWKSGLLTGA
                     AEDPDIDKLSAYTTLHEVLMTLIKLMAPVIPFITEKMYQNLASHFSFGRESVHLEDYP
                     VSDESLIDQGLTERVRLAMQLSSMGRSARSKAGIKVRQPLQDLFVKTRNSTELEMLGL
                     IEDQILDELNIKEIKPVDDASEIVSFRLQPNLPVLGPKYGKSVAAIREALEQADVDTI
                     RTDIEAGNAVNIAGFELGPEDILVNADELEGYSTSTDRGYAVGISTEISDSLKDEGLA
                     RELVHSIQNVRRAAGLEISDRIELFVNGSAEISRVVGKFSDYVLQETLASEVIFGEGL
                     NDSYSESYELEGEQVTISVRKSD"
BASE COUNT          452 a          358 c          267 g          475 t
ORIGIN      
        1 actgattacc ctccaggacc gtatgagccg ttattaggta ttcaaagttg tgttactaga
       61 accgacataa atggaactct gtgggggcca agccaaaaga tatctgccaa ggatgccttg
      121 cgcatttaca cattaaacgg ggcatatgct tcatttgaag aatctcataa aggttcgatt
      181 gaaccgggta aattagccga ttttgtggta cttgaagaag acatattgga tgtggaccct
      241 ttctcgatca aagatatcaa ggttataaag actgtggtag gaggcaaacc tacttacgaa
      301 atttagtccg acttccggac tgatatcgtg acttgttctc cttccaattc ataactctct
      361 gaatagctat cattcaagcc ttctccgaat atgacttcag aagctaaagt ttcttgaagt
      421 acatagtcac taaatttacc cactaccctc gaaatttcag cggacccatt tacgaacaat
      481 tcgatacgat cagatatttc taaccctgca gctcttctaa cattctggat actgtgaact
      541 agttctcggg caagcccttc atctttcagt gaatcggata tttctgtaga tatacctacc
      601 gcataacctc gatcggtaga ggtagaataa ccctccaatt catcggcatt taccaagata
      661 tcttctgggc ctaattcaaa tccggcaata tttactgcat tgcctgcctc aatatcagtc
      721 cttatggtat caacatcagc ctgttccaac gcttctctga tcgcagcaac actcttacca
      781 tatttcggtc ccagaacagg taagtttggc tgtagcctaa aactaactat ttctgaagca
      841 tcatcaactg gttttatctc tttaatattg agctcatcta atatctgatc ctctataagc
      901 cccaacatct ctagctcagt ggaatttcta gttttaacaa acaagtcctg caaaggttgt
      961 cttaccttta tacctgcttt acttcgagct gacctaccca tacttgatag ctgcatggcc
     1021 agcctaactc tctctgtcaa accttgatca atcaaacttt catcactaac tggatagtct
     1081 tccaaatgaa cactttccct accgaaggag aaatgggaag ccaaattctg gtacattttt
     1141 tcagtaatga aaggtatgac aggtgccatt aattttatta aagtcatcag aacttcatgc
     1201 aatgtggtat atgcagaaag tttgtcaata tctggatcct cagctgctcc ggtaagtaat
     1261 cctgatttcc aaaaccttct tcggcttctc ctcacatacc agttggagag atattcgata
     1321 aactcttcaa cttctctagt aaccctctct ggctgaaagt tttcaaggtt gtcagttact
     1381 gtttgaacta aaatatttaa ttcactgaga atccatctat ctaattcggc tcgatctgac
     1441 acgttgggtg attgcgcctc aggatcaaat ttatcaatat tggcgtatgt aacgaaaaac
     1501 gaatatacat tccataaagg aattaaaaat tgcctacgta cctcatcagc ac
//
LOCUS       DASCNS010000111         8289 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000965809, whole genome shotgun sequence.
ACCESSION   DASCNS010000111 DASCNS010000000
VERSION     DASCNS010000111.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000965809"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="QF838_03955"
     CDS             complement(<1..549)
                     /locus_tag="QF838_03955"
                     /inference="COORDINATES: protein motif:HMM:NF021445.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HJO59877.1"
                     /translation="MFQHINGKNSNRIDLVIPPSILTSDTKTYLSRIKESLDQSEMRI
                     LWVDSSDVVHLRNIYGDNIHIEKRENEYIYDPNLVFNLEGGMFRDIRKKINRVTRKAP
                     IFFQLEKNDVPAALEVLGKWRSVQGKKNKFLLDWGYTRAALNSIGDFDTEDLMAWGLE
                     IEKKMVGFSMAGPINQNTACFYV"
     gene            complement(741..1745)
                     /locus_tag="QF838_03960"
     CDS             complement(741..1745)
                     /locus_tag="QF838_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013978.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HJO59878.1"
                     /translation="MNKSEAEDILIQVSKDSRLQEGYQGVRLIMREILRGETVPIHDI
                     SRATGIPVPVVSATRRELEKRKLLSRKGGAILTDTGIKLLNSIGIFDTEIPDFNCQYE
                     ISDTVTKLASQFDELTGQRPSPDYSLDQSHATSLTCFKRVMYFHQSDSIEGRDIAILG
                     DDDLTSLAIVLFATRFGLKINSLTVFDIDERILNFIESVSFKLNFQIGLKKIDFIGEI
                     PPCYKSTQDVFITDPPYTVEGLTRFISVGAEMLKERTNGLGFVSFSNLIPVENARLFR
                     NIASMGLSPQEIIPSFNEYVGAQKHAGISRMGKFFSSGYLEPTNPTSRNLIYTNSKET
                     "
     gene            1899..3545
                     /locus_tag="QF838_03965"
     CDS             1899..3545
                     /locus_tag="QF838_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HJO59879.1"
                     /translation="MLVIDAISQILKKEGVEYLSAFPTTSVIEAAAESGIKPIICRQE
                     RVGVGIADGYSRITAGNPPGVFAMQYGPGAENAYPGVATAFADSVPVLFLPLGHRRDR
                     DRVFPHFNSVDSFNTVTKYVEQLNEAESVTDTMRRAFSRLKNGRPGPVMVEIPIDVAE
                     QEIDASTISQYEKAIIVRSSAEEEAVLRIAKTILSSKTPLIYAGQGVLYSGASQELQE
                     LAELTNIPVTTTMAGKGSIDERHPLSLGSSSIVMNGAVLHYMKTSDTVLAIGTSLTKH
                     GMVTTIPDGKRILHVTNDPIDIGNYYQPDDALQGDAKLVIRQLIEACSDLLSTRDRPE
                     SPANRIREIKSEWSKSWEKKLTSRESPMTPYRVINEFMKATDPSQNIVTHDSGSPRDQ
                     IMPFYKSGGPGTYLGWGKSHGLGTGLGLNMGAKLASPEKFVVNFMGDAAFGMTGLDFE
                     TAVRSGIPILTVVLNNSTMAIETAHMASSHELYKTRNLGGDYADMARAMGGWSERVDD
                     PEEIQTAFLRAKKATENGQSALLEFITSEEQDFSFRGALR"
     gene            complement(3743..4798)
                     /locus_tag="QF838_03970"
     CDS             complement(3743..4798)
                     /locus_tag="QF838_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HJO59880.1"
                     /translation="MVTGTDKASTAQVGETLVEVNNLKMYFPVNEGIIFQRKVAEVKA
                     VDDVTFTIKKGEALGLVGESGCGKTTTGRCILQLYKPTAGEVYFDGVDVTGLNTTGLR
                     QMRRDMQVIFQDPYGSLNPRMTCGDIVGEPLKVHKLTDSKGEYRDRVSELLTTCGLSP
                     YMADRYPHEFSGGQRQRIGIARALAVNPSFIVCDEPVSALDVSIQAQIINLLEDLQED
                     LGLTYLFIAHDLSVVKHISDRIAVMYLGHIVEIAERNELYDNPVHPYTKALLSAVPIP
                     DPLMEADRERIILRGGVPSPMSPPSACSYDPLCPSPDPACDANELAMREISPGHLVAH
                     CAPCVDEHGCYWFPREG"
     gene            complement(4853..5866)
                     /locus_tag="QF838_03975"
     CDS             complement(4853..5866)
                     /locus_tag="QF838_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO59881.1"
                     /translation="MAPLLEVKNLKTHFFTADGIVKAVDGISYEVAEGEVVGIVGESG
                     CGKSVGAMSIMRLVASPPGRIVEGEVLFGGEDLVQMDEREMRGIRGNSISMVFQEPMT
                     SLNPVLTVGRQITETLELHQKMNQKEARERASELLTLVGIPDADTRLDDYPHQFSGGM
                     RQRVMIAMALSCNPQLIIADEPTTALDVTIQAQILELMQDLSRDLGTALIIITHNLGV
                     VARYADRVIVMYAGKIVETSSAVEIYKNPMHPYTIGLLNSVPRLDADDNTRERLEAIE
                     GMPPNLGDRPSGCGFQSNCPAPSPECLTEEPVLREDEPNHWVAYCSHCEQEYGCKWFP
                     RDA"
     gene            complement(5997..6716)
                     /locus_tag="QF838_03980"
     CDS             complement(5997..6716)
                     /locus_tag="QF838_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HJO59882.1"
                     /translation="MYTITLGGMSGGGARDIGPIVAKKLEADYVDRIFLSNIAKDVGA
                     TVEALHQIEEKLPTRSEKWIHVVQRLLERSAVTGSAGDPYFGPGITALLTEEYEDIPQ
                     PTITRGHEVEDDVYIEALESTMKEMAADGDVVFIGRGGHVILKDMPNVLRVGVVASFE
                     DRIKTLMTREAIDKDKAIVTLEQRDQARKYFFKKYFDLDEPDNPNLFHFTINSSEISI
                     NYAAEMVIQNLNSFADGRMFE"
     gene            complement(6829..7341)
                     /locus_tag="QF838_03985"
     CDS             complement(6829..7341)
                     /locus_tag="QF838_03985"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HJO59883.1"
                     /translation="MNLKQHIRDIPDFPKPGILFRDITPLLYHQESFRYCVDAFAKKS
                     EIISPDVIVGIESRGFIFGSALAYEMQLPFVPIRKEGKLPYSVREVKYDLEYGSGILQ
                     IHSDVIKPSQRVVLVDDLVATGGTIQASRALIEELGGVVVGINTVVILKDLCIPELLD
                     DEVLFSLIDF"
     gene            7626..7910
                     /locus_tag="QF838_03990"
     CDS             7626..7910
                     /locus_tag="QF838_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59884.1"
                     /translation="MIGVLTHHWAEQDQVQNARHILDGNGEAQSKALGFINRTTLLSK
                     TDPTQISSLVLWESDQIYDQWKESPARAKAMKDANSVWSRPPESERFDIP"
     gene            complement(7927..>8289)
                     /locus_tag="QF838_03995"
     CDS             complement(7927..>8289)
                     /locus_tag="QF838_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HJO59885.1"
                     /translation="LEDIDYINAHGTSTPLNDASETKAIKLAFGDLAYKIPISSNKSM
                     IGHALGGAGAIEAVAALKTIQSNLIPPTINYNDPDPNCDLDYVPNVARKAKVNTVLSN
                     NFGFGGQNACVVFSSFED"
BASE COUNT         2461 a         1872 c         1595 g         2361 t
ORIGIN      
        1 cacataaaaa caggccgtat tttggtttat cgggccagcc atggaaaatc cgaccatttt
       61 tttctctatc tcaagccccc aggccattaa atcttctgta tcaaaatcac caatggaatt
      121 tagtgcagcc cgcgtataac cccaatccaa cagaaattta tttttcttcc cctgaacaga
      181 gcgccatttc cccaatactt ccaaggctgc aggcacatca tttttttcta attgaaaaaa
      241 tatgggggct ttcctagtta ccctattaat tttttttcta atgtccctaa acattccacc
      301 ttccaagtta aacaccaagt tgggatcata tatatattca ttctccctct tttctatatg
      361 gatattgtct ccatatatat tccgaaggtg tacaacgtct gaagaatcaa cccacaaaat
      421 acgcatttct gattgatcca gagattcttt aatacgtgaa aggtaagtct ttgtgtcgga
      481 agtcagaatg gatgggggaa taacaaggtc aattctatta gagttttttc catttatgtg
      541 ttggaacaaa aagaatgagt ctcccttacg ggtcaaataa agaggccttc cggggggcaa
      601 actataacag gcaaggtaag gagcaaaata aacccaggaa ttccttcctg ttttgctaaa
      661 tatttccttc agtgaagcaa tatcaaagac cgttaagacc ttgtgactgt ctgatatatt
      721 ttgaaataat ttcctcagat tcaagtctcc ttggaattcg tataaatgag gttgcgggaa
      781 gtcgggtttg tgggttctag atatccagat gagaaaaatt tccccatcct acttattcca
      841 gcatgttttt gggctcccac atattcgttg aaagaaggga taatctcctg aggtgatagg
      901 cccatacttg ctatatttct gaacaaacgg gcattttcca ctggaataag atttgaaaag
      961 gatacaaatc ctaaaccgtt cgttcgttct ttcagcatct cggctccaac agaaatgaac
     1021 ctcgttaaac cctcaaccgt ataaggggga tctgtaataa atacatcctg cgtacttttg
     1081 taacatgggg gaatttcccc aataaaatca atcttcttca agcctatttg aaaattaagt
     1141 ttaaagctca cggattcaat gaagtttaat attctttcat caatatcaaa aacagttagg
     1201 ctattaattt ttaatccgaa gcgtgtagca aaaagaacta tggcaagtga agtcaaatca
     1261 tcatccccta atattgctat atcccttccc tctattgagt cactttgatg aaaatacata
     1321 acccttttaa aacatgtcag tgaagtcgca tgagattgat ccagagaata atcaggagaa
     1381 ggtctttgtc ctgtcagttc atcaaattga gaggccaact tggttactgt atctgagatt
     1441 tcatactgac aattaaagtc aggtatttca gtgtcaaata ttccgataga attcaaaagt
     1501 ttaattccag tgtccgtcag aatcgcgcct ccttttctcg aaaggagttt ccttttctcc
     1561 aattctcgac gagttgctga aactacgggt acaggaatgc cagtcgccct tgatatatcg
     1621 tgtataggta ccgtttctcc cctaaggatt tccctcatga ttaacctaac tccctggtat
     1681 ccttcctgca atcgggaatc cttagaaact tgaattaaaa tatcctcagc ctccgactta
     1741 ttcataaatt acaaatatcc ctccataatg ctaattttat gctgttgaac tgaaaaaacg
     1801 taatctccca atttacttca tcttcatatc gcaagtaata gaaatgttat actccgcctc
     1861 atcttagctg taagtacaaa atttagggag agcacgccat gttggtaata gatgctattt
     1921 cacaaattct aaagaaagaa ggtgtggaat accttagtgc tttccctaca acctcagtga
     1981 ttgaggcagc ggccgaatcg ggcatcaaac cgataatctg tagacaggaa cgcgttgggg
     2041 tagggattgc cgacggttat tcgaggatta ctgcaggtaa tcctcctggc gtttttgcga
     2101 tgcaatacgg ccctggagcc gaaaatgcct atccgggtgt ggcaacagca ttcgccgatt
     2161 cagtaccagt cctatttttg ccccttggcc accgaagaga tagggataga gtcttcccgc
     2221 attttaattc agtcgatagc ttcaatacag ttacaaaata tgtcgagcaa ctaaatgaag
     2281 cagaatcagt tacagacaca atgaggagag ctttttctcg tttaaaaaac ggccgtccag
     2341 ggccagtcat ggttgaaata cctattgacg tcgccgagca agaaatcgat gcgtcgacga
     2401 tatctcagta cgaaaaagcg ataattgtac gctcgtcggc cgaggaagaa gccgtactga
     2461 ggatagctaa aaccatactt agctcaaaaa cacctctgat atatgctggg cagggagtgt
     2521 tatattctgg agcgtctcaa gagttacaag aactagcaga attaactaac atacctgtca
     2581 ctaccacaat ggctggcaaa ggttctattg acgaacgcca tcccttatct cttggtagtt
     2641 catctatcgt aatgaatggt gctgttctcc actacatgaa aacaagtgac acagtactgg
     2701 caataggcac cagcctcaca aaacatggaa tggtaaccac aattccagac ggaaaaagaa
     2761 ttcttcatgt cactaatgat cctattgata ttgggaacta ttatcagcct gacgatgcct
     2821 tgcaagggga tgcaaaatta gtcattcggc agttaataga ggcatgttcc gatttgcttt
     2881 ctactcgaga taggccagaa tcccctgcta accggattag agaaataaaa tcggaatgga
     2941 gcaaaagttg ggagaaaaaa ttaacttcca gagaatctcc aatgactcca tacagagtga
     3001 taaatgaatt catgaaagcc acggacccat cccaaaacat agtgacgcat gattcgggca
     3061 gtccaagaga ccagatcatg cctttttata aatcaggagg cccaggcacc tatttggggt
     3121 ggggtaaatc gcatggtctg ggtacaggat taggactgaa tatgggagca aaattagcgt
     3181 ctcctgaaaa atttgttgtg aacttcatgg gggatgcagc cttcggtatg acaggtttgg
     3241 attttgagac tgctgttaga agcggtattc cgattttaac tgtggtcctt aacaattcaa
     3301 caatggccat agaaacggct catatggctt cctcacatga gttgtacaag acaaggaatt
     3361 tgggtgggga ctatgcagac atggctagag ctatgggtgg atggagtgaa agggtcgatg
     3421 acccagaaga aatacaaacc gcattcttga gggcaaaaaa agcaactgaa aacggacagt
     3481 ccgcactcct agaatttatc acctcagaag agcaggattt ttcattccga ggggccttac
     3541 ggtagtgata tattcccccg aagattttgg ggttatttgc aggcacatac ttggataaaa
     3601 aaggttagtt catatgagta atgttttact cttaccatat gtagcaacct cccactaaat
     3661 accttctaac taaaattaac cccagtggaa tgatattttt tccttattac ttgacagtaa
     3721 gaaagggaaa gtaaatttga tcctaccctt ctcttggaaa ccaatagcag ccatgttcgt
     3781 caacgcaagg ggcgcagtgg gcaactaaat gacccggaga aatttccctc atagccagct
     3841 cattggcatc acaagctgga tcaggtgacg gacagagagg atcgtaacta caggccgaag
     3901 gtggagacat cgggctcgga acaccccctc tcaatattat cctttctcta tcagcctcca
     3961 tcagcggatc tggaatcggc acagcagaca acaaagcttt ggtatatgga tgaactgggt
     4021 tatcgtatag ttcgtttctc tctgctatct cgactatatg acctaggtac atgacggcaa
     4081 ttcgatcact aatgtgtttt accactgata aatcatgggc gatgaataag tatgtgagac
     4141 ccaggtcttc ctgtagatct tcaagaaggt tgattatttg cgcctgaatc gatacgtcca
     4201 aagcagagac cggttcgtcg cacactatga aactgggatt gaccgctaga gccctggcga
     4261 tacctatcct ttgcctctga cctccgctaa attcatgggg atatctatct gccatgtacg
     4321 ggcttagacc acaagtagtt agcaactccg aaactctatc tcgatactca cccttgctat
     4381 cagtcaactt atgaactttc aaaggttcgc caactatatc cccacaggtc atgcgaggat
     4441 tcaaggatcc atatggatct tgaaatataa cttgcatgtc ccttctcatt tgacgaagtc
     4501 cggtagtgtt taaaccagtc acatccaccc cgtcaaagta aacttcaccg gctgtaggtt
     4561 tatacaattg caagatgcat cggccagtcg ttgtcttgcc acatccgctt tcacctacaa
     4621 ggccaagcgc ctcacctttt ttgattgtaa aagtcacatc atccacggct tttacctcgg
     4681 caacttttct ctgaaatatg atgccttcat tgactggaaa atacatctta agattgttaa
     4741 cttcaactaa agtttccccc acctgagcgg ttgaggcctt atctgttcct gtaaccattt
     4801 tcactccaca aaacgcgatt aactatttgt aacaaaatct tataggtttt tactaagcat
     4861 ctcttggaaa ccatttgcaa ccatattctt gttcgcaatg actgcaatat gcaacccagt
     4921 gattgggttc atcttctctg agaactggct cttctgttaa acactcaggg cttggagccg
     4981 ggcaattaga ctgaaatccg cagccagaag gtctatcacc tagattcggg ggcattccct
     5041 caatagcttc caacctttct cgagtattat cgtctgcatc caaccgggga accgagttaa
     5101 gtagcccgat cgtgtaagga tgcattggat tcttataaat ctccactgca ctagaggttt
     5161 caacgatttt accggcgtac ataacaatta cacgatccgc atatctagca actactccca
     5221 gattatgagt aattattatt aaggctgtgc ctaaatctct agacaaatcc tgcatcaatt
     5281 ctaatatttg tgcctgaata gtaacatcaa gtgccgtagt cggttcatct gcaatgatca
     5341 gctgcgggtt gcaacttagc gccatagcta tcataactct ctggcgcatt ccaccactga
     5401 attgatgagg gtaatcgtct aaacgagtgt ctgcatccgg aatacctacc aaagtcagca
     5461 attctgatgc tctttctctt gcctcttttt ggttcatttt ttgatgcaat tctaaggtct
     5521 cagttatttg acgacctaca gtaagaacgg gatttagaga tgtcattggt tcttggaaca
     5581 ccatagaaat gctgttaccg cgaatacccc tcatctccct ctcatccatc tggaccagat
     5641 cttcaccacc aaacaatact tcaccctcta caattcgacc gggtgggctt gcaaccaatc
     5701 gcattataga catggccccc acacttttac cacacccact ttccccaaca atccctacta
     5761 cttcaccctc tgcaacttca tatgaaattc catcaaccgc cttcactatt ccatctgcgg
     5821 taaaaaaatg agtctttaga tttttgactt cgagcaacgg agccatacaa cgtcccccct
     5881 tgttgcttct tcaggtttta tatttaattc tggataacca aactttactt tgcgtagcac
     5941 attctaataa acagatccaa tactaacaac ttataaacac aatgattcca cggaaattat
     6001 tcaaacatac gaccatcagc aaaagaatta aggttttgaa taaccatttc agcagcataa
     6061 tttatgctga tttcactaga gttaatggtg aaatgaaata ggttgggatt atctggttcg
     6121 tccagatcaa aatacttttt aaaaaaatac tttcttgctt gatctctttg ttctagagtc
     6181 acaatagctt tgtccttatc gatagcttct cgggtcatca aggtctttat tctgtcttca
     6241 aatgaagcta ccactcccac tcgcaaaaca ttaggcatgt cctttaagat cacgtgtcca
     6301 cctcttccta taaataccac atcaccatct gctgccattt ccttcatggt agattctagg
     6361 gcttcaatgt atacgtcgtc ctccacctca tgacctcgtg ttatcgttgg ctgcgggata
     6421 tcctcatact cttccgttag taaggccgta atacctggcc cgaaatatgg atctccagcg
     6481 gaccctgtca cggccgaccg ctcaagaagt cgttgtacta cgtgtatcca cttctctgat
     6541 cgagttggaa gtttttcctc tatttgatgt aaagcctcaa cagtggcacc cacatcttta
     6601 gcaatattgg acaggaaaat cctatctaca tagtcggcct ccaatttttt cgcaactatc
     6661 gggcctatat ccctcgcacc tccccccgac atacccccaa gtgttatcgt atacatttgt
     6721 accctccaca attaggttta tcccgaatct acatcttatg gagtccgaaa cagtaaaaaa
     6781 acctctcaat cgaatgctga caaacattag tgcgtatgtc aactattatc agaaatctat
     6841 caatgagaat aagacctcat catccaacag ttctggaatg catagatctt tcaaaattac
     6901 gaccgtgttg atacccacta caaccccgcc caattcttca attagtgccc gtgaagcctg
     6961 aatggttccg ccagtggcaa ccaagtcatc aaccaatacc accctttgag agggctttat
     7021 tacatctgaa tgtatttgaa gtatgcctga gccatattcc agatcgtatt taacctcccg
     7081 aaccgaatat ggtaatttac cttctttcct tattggcaca aatggcaact gcatctcgta
     7141 tgcaagtgcg gagccaaaaa taaatcccct cgactctatt ccaacaatca catcaggaga
     7201 aattatttct gatttctttg caaaagcatc gacacaatat cgaaatgact cttgatgata
     7261 gagtaaaggg gtgatatctc taaacagtat tcctggttta ggaaaatcag gaatatctct
     7321 tatatgctgt tttaagttca ttacctaacc tctatgaccg taatatacag ggtattaaga
     7381 tggtgaattt cacagaatgg gaatgggaga gatattatat ggtgagtgag gaaagtgaaa
     7441 ccataattaa aagagattcc gagtccaaat acctatctct tcgcagaaaa caggtatggg
     7501 cttctttcaa atcacatggt ggtgaagtaa tttgtttgag gaaaagtgaa tcttatgaca
     7561 tctttggaca tcgatcactg aattcaattt ttaaagatta atagtaatct cgtggaggta
     7621 gctggatgat aggagtccta acacatcact gggccgagca agaccaagtc caaaatgcta
     7681 gacatatttt agacggaaac ggagaagctc aaagtaaagc tttagggttc attaatagaa
     7741 ccacccttct atccaaaacg gacccgacgc aaatcagttc cttagtgctg tgggaaagtg
     7801 atcaaattta cgaccaatgg aaggaaagtc cagcaagagc aaaggcaatg aaagatgcaa
     7861 attctgtatg gagccgacca cctgaatcag aaaggttcga cataccttaa aagtacctat
     7921 tcaacgttag tcttcaaatg agctgaaaac aacgcatgca ttctgaccac caaacccaaa
     7981 attattggaa agaactgtat tcactttagc ctttcttgcc acattgggaa cataatccaa
     8041 atcacaatta ggatccggat cgttgtaatt gatagtgggt ggaataagat tcgactgtat
     8101 agtttttaag gcagcaactg cctcaatagc gcctgctccg cctaaagcat gcccaatcat
     8161 agatttgttc gaacttattg gaattttata agctagatca ccaaaagcaa gcttgatcgc
     8221 tttggtctct gaagcatcat tcagtggtgt tgaggttccg tgagcgttga tatagtcaat
     8281 atcttctag
//
LOCUS       DASCNS010000112         6828 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000977667, whole genome shotgun sequence.
ACCESSION   DASCNS010000112 DASCNS010000000
VERSION     DASCNS010000112.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000977667"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            310..1248
                     /locus_tag="QF838_04000"
     CDS             310..1248
                     /locus_tag="QF838_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59886.1"
                     /translation="MITFLLRRIIFACFAMIGATFITFGLSRAAGDPRYLYAQEGGYG
                     ITPERWDELGREMGLDKPLVWQYTVWLGNILKGNWQDSLLDRKDVFGKIMDRLPPTLK
                     FALTSYIFAGVIGIPLGVLSAVSRGSLWDYVGRGFALFGQALPVFWVGIMLILIFSVK
                     LRWFPTGTLGEGFFSWKYYVLPTITLGWIAAATYLRLVRSSMLEILDSEFIKLARAKG
                     VSYNTLIWKHGFRNALIAPLTFSSLLLTGFITGAVVTETIFAIPGLGRLAFQAINNND
                     FPIMVGVVLVWTALYVTMNFFTDMIYALVDPRIRYT"
     gene            1258..2343
                     /locus_tag="QF838_04005"
     CDS             1258..2343
                     /locus_tag="QF838_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59887.1"
                     /translation="MAQVELSENLLRQIGAEKSTLGAVWSFIRRWPIVPLFLLALLIF
                     TGIFADLLAPHDPIIARPDNRNIPPVWETPIVLNIKTTSDPLAFPDPVDKPIEEAMVQ
                     FGITPTLENTATQEAVEEAFKEIGYVAKVKWSSWDHILGGDNIGRDLLSRIIHGARVS
                     LVVVTVALIFGLLIGVSMGLVAGYFGGIIDEIIMRVTDIWLGLPFILIAIVAVIAFGQ
                     TFTILIILLGILSWTPFVRNVRGEVLSLKTRDYVSLAKVAGCSTFRILVWHILPGTIN
                     TIIVIATLRVGQLILTEAILSFLGAGIPPPTPAWGAMVNDGRPYINDAWWISFFPGVA
                     IFLVVMSLNFLGDWLRDRLDPRLRQLN"
     gene            2488..3006
                     /locus_tag="QF838_04010"
     CDS             2488..3006
                     /locus_tag="QF838_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59888.1"
                     /translation="MVTMNNKFMMVVLLIALVGIVATACGVKSGNRRPAREDSDKTGE
                     FHRSPISSKLQELSVDLDKADGTCKATPGDLTSPVASRIRLGVQLVGTSVKEGVTKSI
                     EIVGEKKQAKYEISGMTIKAAGGALAIGTSSIALDLASGSRKNYDFNVGAPGTYDILC
                     DGDKVGTFTATE"
     gene            3088..5121
                     /locus_tag="QF838_04015"
     CDS             3088..5121
                     /locus_tag="QF838_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59889.1"
                     /translation="MNRFAYIIRTLIVISLIMNVCILAGDHRTTPEILYAAIIPSWAV
                     YFLAAAVPLSVVLSSLVTALLIRKNVPATIDSIDRPDSLIEVATPMVVPEPESTPKVA
                     VEQPGRGPIPINVHEVPTTSPANSEAPIFVPTISTFEVTENPTDLFFHEGTIWVASQD
                     EDGIANYSMDGELIYSMPLHPYPNSLGHDGDELWVGTYFAVRTFDLKGGMGSAPVELR
                     RPTDMLYAGGAMWIANSGRDVVTMVTKDRQTVKNIQSGAKPQKLTFDGQHIWVVNNGD
                     DSISKIDQEGNLIGTWNTGGGARGIAFAENHIWVSNSLDDTLSRFTLEGDKVADYPTG
                     TLPGDVVYDGEGIWVANRTDKTVTKYGIEGQHLGTFHIGNTPNSLATDGKGTVWAAHS
                     AEGLVSKLVVEDVTISTYSVGNAPEPIIFDGDNLWVGNALSHTIMKIGLDGQQQAVYE
                     SHGREPNALLFDGENIWSANQFDHNATRLSRGGELLGTYPVSTLPRTLAFDGKNIWTS
                     GCWETLLYRLDLEGNSVSPVETEGAGPIVLFFDGENIWAANAHSDSVTKFSTEGNPEG
                     NFSVGDVPIAFTEEGDNIWVANWREHTVSKLSKTGEDLGRFDTGRLPYGIAFDGENIW
                     TANSMDGTVTKLSLNGEILATYPVGAAPAKIIPVNGEIWVTLTSDDSIVKLIP"
     gene            5154..5945
                     /locus_tag="QF838_04020"
     CDS             5154..5945
                     /locus_tag="QF838_04020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO59890.1"
                     /translation="MFHMVDEQGSSAWAGICDLVYPDRRDDIGYYLDQASQKGSSILE
                     IGCSTGRITELLADFGKEVHAVDPSSGMLSIAQKKIEKLSNPGQVSFSESPLPDLKLT
                     SDKKFSLAIVPSLAFMSILSPADQQKFLHNVRRYLSSAGKLIIEMKVPDPDVILGDPA
                     TLYHLMDINNARDGGKLIFYHQRDYDDYEQIGDSKVEVEFVDSDGIVVQKVVHDLSFR
                     YTFRWEMYHLLRICGFEILSLHGDFDGNDFDEKSDSMIWVAGSRS"
     gene            complement(5969..>6828)
                     /locus_tag="QF838_04025"
     CDS             complement(5969..>6828)
                     /locus_tag="QF838_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJO59891.1"
                     /translation="QNLYPRLELSTSIFRSSLAIFVVLLLSLACDSQSFDTQLNQAKI
                     FRTQGNLEEAEQLLTELIQSNRSYPEALLLRGQIRESNSEFEKAITDYSAIIDQNPEL
                     TQAWALRGTCYYRIGVFQNAIHDFSRAIELEPDNADLYLYLGNSYGEMDLFTKAIKNF
                     NIAREISFGDYYSNLAKAYEDINLQKYTSALARATSAIKENPTDPIPHSYKGISLYHL
                     GKFNEAILYLDAAIKLDPNNSVTIYNLGLAHMASKNTEEAVFQFEKAMSINPSLKHDI
                     ELTGALEDD"
BASE COUNT         1886 a         1280 c         1648 g         2014 t
ORIGIN      
        1 tggtgataat gaagtaagtg ctgacctaga aaaatagacg ccccttgcat tgagcagggg
       61 gcgtttcttt gtagattcag gagagccaac cctctcaact taaatcccat cgcatagaaa
      121 atagctaata aatacagaaa tggttggcag ggagatagtg aaaaagatat acttctccct
      181 ccccgccgtg gcctttgcca aatttaattg ggtaatttaa aatcaaccca tgggcggaag
      241 cctgttagaa tggcaaaata aagttggacc gttgcaatag cagcggagtt tttggaaggt
      301 gctctaagga tgattacatt cctattgagg cgaataattt ttgcttgttt cgctatgatc
      361 ggagcaacct ttataacttt tggactctca cgagcagcgg gtgatccccg ctacttatac
      421 gcccaagagg gcggttatgg aattacccct gagcgctggg atgaactagg gcgcgaaatg
      481 gggctggaca aaccactcgt atggcaatac acagtttggc ttgggaatat tcttaaaggc
      541 aattggcaag atagccttct tgaccggaag gatgtgtttg gaaagatcat ggataggtta
      601 cccccaacac ttaaatttgc gttaacctca tatatatttg cgggtgtcat tggcattcca
      661 ttgggagtac tatcggctgt gagccgggga agcctgtggg attatgtggg taggggattt
      721 gctctatttg ggcaagctct accagttttt tgggtgggaa taatgctgat tttgatattt
      781 tcggtgaagc tgagatggtt tcccactgga acgctgggag aaggcttttt ctcatggaaa
      841 tattatgttc taccgaccat aaccttagga tggattgccg ccgctaccta tcttaggctt
      901 gttaggtctt ctatgctgga aatactagat tcagaattta ttaagttggc tagagctaaa
      961 ggtgttagct acaacacctt aatttggaag catggtttta ggaatgcttt gatagcgcct
     1021 ctcacattct ccagcctgct gcttacgggt tttataaccg gtgccgttgt gacagagact
     1081 atatttgcca taccaggatt gggaagactt gcctttcagg cgataaacaa taacgacttt
     1141 ccgataatgg taggcgtagt tttggtatgg accgcgcttt acgtcacaat gaactttttt
     1201 acagacatga tttacgcatt agtagatccc agaattcgat acacctagga ttaaaagatg
     1261 gcacaggtag aattatcaga aaaccttcta aggcagattg gggctgaaaa aagtacctta
     1321 ggggcggtct ggagcttcat aagaagatgg ccgattgttc ctttgtttct tttagcgttg
     1381 cttatattca cggggatttt tgctgacttg ctagccccgc atgaccccat tattgctaga
     1441 ccagataaca ggaatatccc tccagtgtgg gaaaccccta tcgtgctcaa tataaaaact
     1501 acttccgacc cgttggcttt tccggaccca gtcgataaac ccattgaaga agctatggtg
     1561 caattcggca taactcccac cttggaaaat accgcaaccc aggaagctgt agaagaggcc
     1621 tttaaagaaa tagggtatgt ggctaaagtt aagtggtcta gttgggatca tatacttggc
     1681 ggggacaaca tcggccgtga tctcttaagc cgaatcattc atggcgcaag agtatctctg
     1741 gttgttgtga cagtagcttt gatatttggc ctcctcattg gtgtgtcaat gggattagtg
     1801 gcgggttact tcggtggcat aatagatgaa attattatga gggtgacgga tatatggcta
     1861 ggactgccct tcatattgat tgcgattgtg gcagttatag catttggaca gacctttaca
     1921 attttgatta ttttgcttgg aatactgtct tggacacctt ttgtaagaaa tgtacgaggt
     1981 gaggtgctca gcctaaaaac tagggactac gtgtccttgg caaaggttgc agggtgttcc
     2041 acattcagaa tactggtctg gcacattctc ccgggaacaa ttaatacgat aattgtgatc
     2101 gcaactcttc gggtcggtca gcttatattg actgaagcta tattgagctt cttaggggcg
     2161 ggtataccac cccctactcc tgcttggggt gccatggtga atgacggtag gccctacatc
     2221 aacgatgctt ggtggatatc gttctttcct ggtgttgcta tattcctagt cgtaatgtca
     2281 ttaaatttct tgggtgactg gctaagagat agattagacc ctagattaag acaactcaat
     2341 taaaagaaag gaaaaggagt gaggcttaat tgtctggctc cttttccttt ttaaaatgtg
     2401 cgggaatttc attagagagt ggtaaacttc agcaccactg gaataggtta agagatcgtt
     2461 aatcattttc atgcaatgga gcgaattatg gttactatga acaataaatt tatgatggtg
     2521 gtcctactga tagccttagt aggtattgta gccacagcat gtggtgtcaa atccggcaac
     2581 agaagaccag cccgagagga ctctgataaa acaggtgagt ttcataggtc accaatatca
     2641 agcaaactac aggaattgtc ggttgattta gataaggctg atggcacctg taaggcaact
     2701 cctggggatc tgacttcacc ggtagcttct agaattcggt tgggcgtgca actcgtaggc
     2761 acatcagtga aagaaggggt tacaaaatcg attgagatcg tcggtgagaa aaagcaggct
     2821 aagtacgaga tttccggtat gaccattaaa gctgctggcg gtgccttggc gattggaact
     2881 tcgtcaatag cacttgattt ggcgtctggc tccagaaaga attatgactt taatgttggc
     2941 gcccctggaa catatgatat tctatgcgac ggagataaag tcggtacatt cactgctacg
     3001 gagtaacgcc cgtcattaaa tttatcgagt taaaagcaag gcccctagac ttagtgctaa
     3061 gtacaggggc ctttttttga ggaaaaaatg aatagatttg cctacattat tcggactctt
     3121 attgttatct ctttgattat gaatgtttgt attttagccg gggaccatcg cacgactcca
     3181 gaaattttat acgcagcgat catcccctct tgggctgtgt atttcttggc tgctgcggta
     3241 ccactctcag ttgtgttgtc gtctcttgta acagcgttat tgatcagaaa aaatgtcccg
     3301 gctactattg attctataga ccggcctgac agcctcattg aggttgcaac gccgatggtt
     3361 gttcctgaac cggaatcgac tccaaaagtg gccgttgaac aacctggacg cggccctatc
     3421 ccaattaatg tccatgaggt tccgacaact agtccggcca attcagaggc cccaattttt
     3481 gttccgacaa tatctacatt tgaggttaca gaaaacccta ccgatctgtt tttccatgaa
     3541 ggcacgatat gggtggcttc acaggatgag gatggtattg ccaattattc tatggatggt
     3601 gaattgattt actcaatgcc acttcaccct taccccaatt cgttgggcca tgatggcgat
     3661 gagctttggg ttggaactta ctttgctgtc agaacctttg atttaaaggg tggaatggga
     3721 agcgctccgg ttgagcttcg ccgaccaacg gatatgttgt atgcgggcgg cgcaatgtgg
     3781 attgcgaatt ccggaagaga cgtagtcact atggtgacta aagatagaca aactgttaag
     3841 aatattcaat ctggtgcgaa accacaaaaa ttaacatttg atggtcagca catatgggtt
     3901 gttaataatg gagatgattc gatttccaaa attgatcaag aaggcaattt gattggtact
     3961 tggaatactg gtggtggtgc aagaggcatc gcatttgctg aaaaccacat atgggtaagc
     4021 aattcgcttg atgatacttt aagtcgattc actttggaag gagataaagt agcggattac
     4081 cctacaggaa ctttgccagg tgatgttgtt tatgatgggg aaggaatttg ggtagcaaat
     4141 cggacagata aaacagtcac aaaatatgga atagagggac agcatttagg gacttttcat
     4201 atagggaata cccctaactc cctcgcgaca gacgggaaag gtactgtgtg ggctgctcat
     4261 tccgcggaag gactggtatc aaaattggta gttgaggatg tcactatttc cacttattcg
     4321 gtaggaaatg ccccagagcc aataatcttt gatggtgaca atttatgggt aggaaatgcc
     4381 ctatcccata ccattatgaa aattgggttg gatggacaac agcaggctgt atatgaatcg
     4441 catggtaggg agcctaatgc cttattgttc gatggggaaa acatatggtc tgccaatcaa
     4501 tttgatcata atgcaaccag actttcacgt ggaggtgaac ttttaggcac atacccagtt
     4561 tccacactac caagaactct tgcctttgat gggaaaaata tatggacttc aggatgttgg
     4621 gaaacgttgc tgtacagact agaccttgaa gggaactcag tgtcccctgt ggaaactgag
     4681 ggagctggac ccatagtgtt gttcttcgat ggcgaaaata tttgggccgc aaatgcccac
     4741 agtgatagcg taaccaaatt tagcacagaa ggtaaccctg aaggtaattt ttctgtcggt
     4801 gatgttccaa tcgcgtttac ggaagaagga gataacatat gggtagcgaa ttggagagaa
     4861 cacactgtat ctaaactctc gaaaactggt gaagatttag gaagattcga tacgggtagg
     4921 ctgccgtatg gtattgcatt tgatggagaa aacatatgga cagccaatag tatggatggg
     4981 actgtgacga aactttccct caatggggaa atattggcca cctatcctgt gggcgcggca
     5041 cctgcaaaaa taattcctgt gaatggagaa atttgggtta ctttaacgag tgatgattct
     5101 atagttaagc tgatccctta aaaagactat tggtataagc aagaggttga ataatgtttc
     5161 acatggttga tgagcaggga agttccgcct gggccgggat atgtgatctg gtttatcctg
     5221 atagaaggga tgatatcggt tattacttgg accaggcttc ccagaaaggt tcttcaattt
     5281 tagaaattgg ttgttcaaca ggacgtatta cagaattact tgcagatttt ggtaaggaag
     5341 tacatgctgt ggacccatcc tcgggaatgt tatctatcgc ccaaaagaag attgaaaaac
     5401 tatcaaaccc tggacaagtt tcatttagtg aatcgccttt gccggacctc aaactaactt
     5461 cggataaaaa gttttcctta gctatcgttc catcattggc atttatgtct attctttcac
     5521 cggcagacca acagaaattt ctccataatg tgagacgcta tctcagttct gcaggaaaac
     5581 ttattattga aatgaaagtt cccgacccgg acgtaatttt gggcgacccg gcaactctct
     5641 atcaccttat ggatataaat aatgcccgtg atggagggaa attaattttc tatcatcaga
     5701 gggattatga tgactacgaa caaattggtg attcaaaggt tgaagtggaa tttgttgata
     5761 gcgacggaat tgtcgttcaa aaagttgttc atgatctatc tttccgatat acgttcaggt
     5821 gggaaatgta tcatttactt cgcatttgtg gatttgagat attatccttg catggggact
     5881 ttgatgggaa tgattttgac gaaaaaagcg atagtatgat ctgggttgcg ggttctaggt
     5941 cttgattata gttgatacta tgttggaact aatcatcttc taaggcccct gttagctcta
     6001 tatcgtgttt tagagagggg ttgatactca tcgccttctc aaattgaaaa acagcttctt
     6061 ctgtattttt agaggccata tgggctagac ctaaattata gatagttact gagttatttg
     6121 ggtctagttt aatggctgca tctaaataca agattgcctc attaaacttt cccaaatggt
     6181 atagtgaaat tcctttgtat gaatgaggta ttgggtctgt aggattctct ttgatcgctg
     6241 aggtagccct agctaaagca gaggtgtatt tctgtaaatt gatatcttca taggccttgg
     6301 cgaggttact atagtagtct ccaaatgata tttctctggc aatattaaaa ttttttattg
     6361 cttttgtgaa caggtccatt tcaccatatg aattcccaag atataaatat aaatctgcat
     6421 tatcaggctc taattcaatt gctcttgaga aatcatgaat ggcgttttga aaaactccta
     6481 ttcggtaata gcaagttccc cttagggccc acgcctgggt taattcggga ttttggtcaa
     6541 ttattgccga ataatcggtt atagcttttt caaattcgga attgctttcc cgaatttgcc
     6601 ctctcaacag gagagcttct gggtaacttc tattcgattg tattaattca gtgagtagct
     6661 gctctgcttc ttcaaggtta ccttgggttc gaaatatctt ggcttgattc aattgtgtgt
     6721 caaagctttg ggaatcacat gccaaggaaa gaagcaatac aacaaaaatc gcaagtgagg
     6781 aacggaaaat actggttgat agttccagtc tagggtataa attttgcg
//
LOCUS       DASCNS010000113        10214 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000979019, whole genome shotgun sequence.
ACCESSION   DASCNS010000113 DASCNS010000000
VERSION     DASCNS010000113.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000979019"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="QF838_04030"
     CDS             complement(<1..883)
                     /locus_tag="QF838_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HJO59892.1"
                     /translation="MNKTVGILLIIFLLIVACNRSENTEPSTITEISTTSIDTLEKSL
                     IGGNETLSSGVDTDIEPPAPAEVTPVVLPPLDIPPAVIADLISVSQDDDVMTTVEAVR
                     ELAPSVVHISTDTAVISLYNQIVPQTGVGSGIILDKAGNILTNNHVVEGADFVTVTLN
                     DGRSYPATLVGSDNITDLAVIKISAAKLNPAKFGKSSDLQVGEDVIAVGHALGLKGGP
                     TVSKGVVSALERTIQTDAQKAMVDLIQTDASINPGNSGGPLANSSGEVIGINTAIIDG
                     SNGIGFAINIDDSHVVVD"
     gene            complement(892..1749)
                     /locus_tag="QF838_04035"
     CDS             complement(892..1749)
                     /locus_tag="QF838_04035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HJO59893.1"
                     /translation="MAEQKLQLPVSISGKNAWDVAREISLSAGEILLDWWPKSKEVSD
                     KGQNDIVTNVDRECEAHIREALASHFPEHGIFGEEEKGDDPTTGWTWIIDPIDGTQNY
                     AWGIPCFSIVIALAKDGEVVAGVNFDPINSEMFHAARGKGAFLNDQKIKVSEISNFED
                     VIMVTDPAYGPVAGTTDTLQMLRKIWPSLKTARMIGSSALSISYVAAGRMDIFVHHRL
                     QPYDQAAGLILMEEAGGLVTDREGTRAQLYSDGLIASSSIIHQQFMEITKHLQWRKPS
                     SRSLNPRER"
     gene            1941..2966
                     /locus_tag="QF838_04040"
     CDS             1941..2966
                     /locus_tag="QF838_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HJO59894.1"
                     /translation="MSSPLYKQMIFRFSIALLVMGIFSAGIGCTNRSDTFTVSKNPSD
                     STPEGKGSGNINKGGLLEIHTTTSIISDWVRVVGGERVLVESLTPAGKDPHTYQPTPK
                     KVASLSNSKLVFAIGLSLEGSNITPLLENSLNPNATFLVLGTKVDPIKLMHHDEHDKD
                     EEHDEHLHGPIDPHFWLDPSRVSLAIDEIKTQLSKMDPDSSDYYQKNASAYKGQLSDL
                     DQHMRSVFEKIPEKDRKLVTSHEALGYLEETYGFDVIGAVIPAATTDSGPTAANIAKL
                     IDEIKLTKVKGIFMEEGVEEKITEQIAQDANVQVITGLRVEYLKEGESYVEMMMALSE
                     LIHNGLK"
     gene            3451..4881
                     /gene="dnaA"
                     /locus_tag="QF838_04045"
     CDS             3451..4881
                     /gene="dnaA"
                     /locus_tag="QF838_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HJO59895.1"
                     /translation="MDRETIQKANKIWNSVLGKVELSIPKPSYDTWLRNTVGVSFTSN
                     EFNVETPNAFTAQYLEDRMLDMLEQELRTTIGFNVKLKFTVINEIIPSEENEQSVTSS
                     SVYSRSDQTAKYSTSNQGMFQDPNFDNVVDARIGSYDFNTRYTFENFIVGNSNQLAFA
                     GAKAVSENPGTIFNPLVIHSSVGLGKTHLLQAIGHDTKKLGLTVIYVTSEEFTNQYVR
                     AIQNGQTEEFRNKYRTVDVLLIDDIQFLIGKEQTQEGFFHTFNSLHVENKQIVVASDR
                     NINELRTLESRITSRLTGGLVTDIQAPQLETRIAILRDKAAQQGYDIPANVIEFLGNK
                     IRSNIRELEGLLNRVVALAQFRDEPITVESISRIVGDSERLNKEPISDSKIISEVSNY
                     FGVTSDQIMGPLRTKTYLVPRQVAMFLLREETSLSLSAIGKILGGRDHSTVIHGHEKV
                     ASLINVDDKLRTDILAIRNNLTGTAG"
     gene            4923..5576
                     /locus_tag="QF838_04050"
     CDS             4923..5576
                     /locus_tag="QF838_04050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJO59896.1"
                     /translation="MRLILIRHGETDANLLMRWSGSKDLEITNLTTAGKSQAQELGRW
                     FEKEDFIPTHVYVSPQKRAQDTARLAGGHWNISFVTIDDLKETGAGIFEGLTWNEIEA
                     RYPKQAANFKNSRDWSYVEDSEQETDRRNRANRILDLALSHHSDGDTVVMFCHAGIIQ
                     HTVSCIFESPKLWAIATKNTAIFDFQLDASKWSDLSRQRFNPTSWRILRFNEQPHLS"
     gene            complement(5579..6220)
                     /locus_tag="QF838_04055"
     CDS             complement(5579..6220)
                     /locus_tag="QF838_04055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJO59897.1"
                     /translation="MRLILVRHAQSKANAEGRWQGTMDFDLSKEGQGQCEKLRERFVK
                     EEVEPSHVYSSPLTRAHKTAEISLPAAQVHKIQDLRENDAGVFSGKTSDELSTEYSAI
                     FEEFRRSRNFDLVPKAETRYQIRQRAETVIGFLVKGHNSKDFVIAYSHSGFLVHLIAV
                     ILGTNRVWNLRIPNTAIFDFDIDPDGWDVSKSINGDRRRFQINRFADAGHLFS"
     gene            complement(6217..7734)
                     /locus_tag="QF838_04060"
     CDS             complement(6217..7734)
                     /locus_tag="QF838_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59898.1"
                     /translation="MISFLSRIQDKPSYRWWIYGAVAIGTFISIVEQSATSIVVPSIA
                     EHFGADIPTAQWMAIAYMLSVSALMMPAGAIADSIGRKRIWIFGLSIFACATAITGFS
                     QEFWMVIVGKIFMGIGASALQANGMAMIAGSFPDEERGKALGLHMTIVGLGAIGGPVL
                     GGSIDSYFGWRAIFIFIAVFSIFALCIAMLILTPDPQKSPGKADPIKNFDWSGTFISA
                     LFLLSLMLVITFSNKLSLSSPYIILGTMCSILLFAAFLFREKTAKNPILPLSLFKSLS
                     FSLGSSSRFISFTASSSTFFLMPFFLVSGLGLTTATAALYLLPGSILMAIFGPLSGRL
                     ADRIGTTGPSLIGMAFCAVAMYLFSEVSLTSTLALVGIASGLSGMGMSIFMAPNTSAI
                     MGSAGRKHYGIVSAFLNLTRNGAHIVGIAIPTAIVVSVMAGLGYEADLSDPEKLKDIG
                     LRTAYASAMAKAFQLSTILMICAGLLVVADGIFDRLRKQANQLKSDTLPPLGIKK"
     gene            complement(7747..8316)
                     /gene="pyrE"
                     /locus_tag="QF838_04065"
     CDS             complement(7747..8316)
                     /gene="pyrE"
                     /locus_tag="QF838_04065"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HJO59899.1"
                     /translation="MNDIDRLLEVAKERKALLFGEFILSSGNKSSYYFDGRLLTLDPE
                     GSYLTAKCFLPILYKCSAEALAGPTLGADPIVSSVSTLSYLEGKPVPALIVRKEKKTH
                     GGGRNIEGHFYEGQKVAVVDDTCTTGGSLFHAIEAVEDEGCEVVKVLSIMDRMAGGSE
                     ELKRRGYDFESLFVADDKGEIRITQIGAQ"
     gene            complement(8358..8615)
                     /locus_tag="QF838_04070"
     CDS             complement(8358..8615)
                     /locus_tag="QF838_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59900.1"
                     /translation="MDEWQDPDLLHSECEVVGLTRPGFQIDKSKSYLSRQRKYSLRTI
                     EVNSPEISSTRVRNLIQDGVDVADLIPQEVNEYIKEQKLYH"
     gene            complement(8617..8943)
                     /locus_tag="QF838_04075"
     CDS             complement(8617..8943)
                     /locus_tag="QF838_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HJO59901.1"
                     /translation="MFVGLFGGTFDPIHNGHIEAANSAHLILKLDKVIMVPAGDPYLK
                     RTKLVASPKERYRMVSLAVRKIPSLEVSDIEIFRPGPSYTIDTVRELKREGHEVIVFT
                     WDGLNN"
     gene            9070..10214
                     /locus_tag="QF838_04080"
                     /pseudo
     CDS             9070..10214
                     /locus_tag="QF838_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012935.3"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
BASE COUNT         3137 a         2249 c         2012 g         2816 t
ORIGIN      
        1 gatcaaccac tacatgcgaa tcatcaatat ttatggcaaa cccaattcca ttactcccat
       61 caatgattgc cgtgttgatt cctatgactt ctccactgct attggccaaa gggcccccac
      121 tgttgcctgg attgattgag gcatctgttt gtatgagatc aaccatggct ttttgggcat
      181 ccgtttgtat ggtacgttca agagcactga cgacaccttt actcacagta ggtcctcctt
      241 tgaggcctaa ggcgtgacca acggcgataa catcctcacc gacttgtaaa tctgaagatt
      301 taccaaattt tgcaggattc agcttggccg ccgatatctt tattaccgct aagtcggtga
      361 tattatcact cccaactaat gttgcaggat aagaacgacc atcatttaaa gtaacagtca
      421 caaaatctgc gccttccacg acgtgattgt ttgtcagtat gttacccgcc ttatcgagta
      481 taatccccga tcctacccca gtttgaggaa cgatttggtt gtataaactg atcacagctg
      541 tatcagttga tatatgaacc actgaaggag ctagctctcg gacagcttca acggtagtca
      601 taacatcatc atcctgggaa actgatatca agtctgcaat gactgccggt ggaatatcca
      661 aaggtggtag aacaacagga gtaacctctg ctggtgctgg aggttcaata tccgtatcaa
      721 cgcctgacga taatgtctcg ttgcccccaa tcaaggattt ttctaatgta tcaatgcttg
      781 ttgtggaaat ttccgttatc gtcgatggct cggtattttc gctccgatta caagccacta
      841 ttagcaagaa tattattaat aggatcccaa cagtcttgtt catgttttct actatctctc
      901 ccttgggttg agtgacctac tggatggttt tctccactgc aaatgttttg ttatctccat
      961 gaactgctgg tggattattg aagaagaagc tattaagcca tccgaataaa gttgagctct
     1021 ggttccttct ctatctgtta ccagtccccc cgcttcttcc atcagtatta gtccggccgc
     1081 ctggtcataa ggttggaggc ggtggtgaac aaaaatatcc attcttccgg ccgccacata
     1141 agagattgac agagctgagg acccgatcat tcgggcggtt ttaagggaag gccaaatttt
     1201 tctcaacatt tgtaaagtgt ctgtagtacc tgcgacaggg ccatatgccg ggtctgtcac
     1261 cataattaca tcttcgaaat ttgaaatctc acttactttt atcttttggt cattgaggaa
     1321 agcccctttt cctcgggccg catggaacat ctcgctattg attgggtcga aattaacacc
     1381 tgctacaact tccccgtctt ttgcaagggc tataacaatg gagaaacaag gaattcccca
     1441 agcatagttt tgggtaccgt cgatagggtc gatgatccac gtccaacctg ttgtggggtc
     1501 atcacccttt tcttcctccc caaaaattcc atgttcagga aagtgacttg caagggcctc
     1561 cctaatatgc gcttcacatt ctctgtcgac gtttgtgact atgtcgtttt gacccttatc
     1621 agatacttcc ttcgattttg gccaccaatc caacagtatt tctcctgcag ataaagatat
     1681 ttccctcgct acatcccagg cattcttccc ggatattgat actggcaatt gaagtttttg
     1741 ttctgccaat ttattcctct cgttttatga ttttaatcat tccagaatat cttaatattc
     1801 taaaacaatc ctaagatttt gaaactatat tcgacttata gcggatgttg acaagaaaca
     1861 gaaatgacac ttaaaatatt tacttaatga tactgagtct caataaagat aaaccaatgg
     1921 ccaattcagg ttcgaataat atgagttcac cgctatataa acagatgatt tttagattta
     1981 gcatagccct cttggtgatg ggaatttttt ctgcaggcat cggctgcacc aacagatccg
     2041 atacattcac tgtgagcaaa aacccttccg attcaacccc agagggtaag ggctctggaa
     2101 atataaacaa aggtggtttg ctagaaatac ataccaccac gagcattatt tctgattggg
     2161 ttagggttgt aggaggagaa agggtgctag ttgagagttt aacacctgcc ggcaaagacc
     2221 cacataccta ccagccaacc ccaaaaaaag tagccagcct atctaattcg aaattggtgt
     2281 ttgcaatagg gttgtcatta gagggcagca atattacacc gcttcttgaa aattcattga
     2341 atccaaatgc cacatttcta gttttaggga ccaaagttga tccaattaaa ctgatgcacc
     2401 atgacgaaca cgataaagac gaagaacacg atgagcatct tcatggaccc attgatcctc
     2461 atttctggtt agatccgtcc cgtgtgtccc tagctataga tgaaatcaaa acccaacttt
     2521 ccaagatgga ccctgattca tctgactact accaaaaaaa tgcctcagcc tacaaaggtc
     2581 aactttctga ccttgatcaa catatgcgat ctgtgtttga aaaaatccct gaaaaagacc
     2641 gaaaattagt aacctctcat gaagctcttg gctacctgga agaaacatat ggttttgatg
     2701 tcataggtgc cgtaattcct gcagcaacta cagattccgg accgaccgcg gcaaatatag
     2761 caaagctaat cgacgaaatt aaacttacga aagttaaggg tatttttatg gaagaagggg
     2821 ttgaggaaaa aataacagag cagatagccc aagacgcaaa tgttcaagtt ataacaggat
     2881 tgcgagtgga atatttaaaa gagggcgaat catacgttga gatgatgatg gcactttcag
     2941 aattaatcca taacggattg aaataaattc catgattatc acatggcttt cctttgagtg
     3001 tgaaaaatag cgaacataat catggaattc tcttctttct ctatgttttc aaatacatat
     3061 ataagggtta ctataaatat cctcattaac acatgaattg tgtttcatga acgacaattt
     3121 cgtcctttaa tagcttgaaa caaattgacg tcgaaatacc cctttgtccc ccttgcacag
     3181 tgttaaatat ggggtgtaca acatacatct gaaaagaagt tcaattttag ttgaatttca
     3241 ggagttctgg agagtctcga aaatatacaa agtcatctaa agtgagctct ccgtgaaatt
     3301 ttccgggttt atgtcttatt caaacctgtt catatcaaga gcagcattga tgtaagtagg
     3361 tggggattga agttgaggag tcgggaaatc tccgatattg ccttttgaaa aaaggggctc
     3421 aaataccgaa ggtaatattg tgtaaaattt atggaccgag aaacgattca aaaggctaac
     3481 aaaatctgga attctgttct aggtaaagta gaactgagca ttccaaaacc tagctatgat
     3541 acttggcttc gaaacaccgt tggggtttcg tttacttcca atgaattcaa tgtcgaaact
     3601 ccaaacgcat tcacagctca atatttagaa gatcgcatgc tagatatgct tgaacaggaa
     3661 ttaagaacaa caatcgggtt taacgttaag ctcaaattca ctgtaatcaa tgaaatcatc
     3721 ccttccgagg aaaatgagca atctgtaacc tcgtctagcg tatacagcag gtcagatcag
     3781 actgcgaaat actcaacttc taatcaaggt atgtttcaag acccgaattt cgataatgta
     3841 gttgacgcac ggattggatc ctatgacttt aatacaagat acacattcga aaattttatt
     3901 gtcggaaact ccaatcagtt ggcctttgcc ggagcaaagg ccgtttcaga aaaccctgga
     3961 accattttta accctttagt aattcattca tccgtaggtt taggaaaaac acacttatta
     4021 caagcaattg ggcacgacac caaaaaacta ggcctgactg tgatctatgt gacctctgag
     4081 gaatttacga atcagtatgt aagagcaatt cagaatggac aaactgagga attccgaaat
     4141 aaatacagaa ctgtcgatgt acttctaatt gatgacattc aatttctaat aggaaaggaa
     4201 caaacacagg aaggattttt tcatactttc aactctctgc acgtggaaaa caaacaaata
     4261 gtggttgcaa gtgatcggaa tattaatgaa ctaagaacct tggaatcacg tatcacctca
     4321 cgactgactg gcggattggt aactgatatt caggcacccc agctagaaac cagaatagct
     4381 atactaaggg ataaggcagc ccagcaaggg tacgatatcc ctgcaaatgt aatcgaattt
     4441 ttaggaaata aaattcgttc aaacattaga gagttagaag gccttctaaa tagagtcgtt
     4501 gctttggctc agtttcgaga tgaacccatt acagttgagt ctattagccg aattgtggga
     4561 gattcggaaa gactcaacaa agaacctata tcggattcca aaattatttc ggaggtgtcg
     4621 aactactttg gtgttacctc cgatcaaatc atggggccgc tcagaacaaa aacatatttg
     4681 gttcctagac aagtcgcaat gttccttctt agagaagaaa catccttgag tctttctgca
     4741 ataggcaaaa tacttggtgg aagagatcat tcgaccgtaa tacatgggca tgaaaaggtc
     4801 gcctctttaa ttaatgtaga cgacaagctc agaaccgata ttttagctat tagaaacaat
     4861 ttgacaggta cggccggata atccaaatgg tcctccactg gaacggctaa cgggttttat
     4921 tcatgagact cattttaatt agacatggtg aaaccgacgc aaacttattg atgcggtggt
     4981 ctggctcaaa ggaccttgaa ataaccaatc ttacaaccgc cggcaagagt caggcacagg
     5041 aactaggacg ttggtttgaa aaggaggatt ttattcctac acatgtgtac gtgagtccac
     5101 aaaaaagggc tcaagacact gctcgtttag caggtggaca ttggaatatt tcctttgtca
     5161 ccatagacga cttaaaagag actggtgcag ggatctttga gggactgacg tggaatgaaa
     5221 ttgaggcaag gtacccgaaa caagcagcga attttaaaaa tagtagggat tggtcttatg
     5281 tcgaagattc agagcaggaa accgaccgga ggaacagagc caatagaatc ttggatttag
     5341 ctttatccca ccactctgac ggcgatactg tagtcatgtt ctgccatgcg ggaataattc
     5401 aacacacagt gagctgcata ttcgaatccc caaagctctg ggccatagcc acaaaaaaca
     5461 cagcaatttt cgatttccaa ctggatgcca gtaaatggtc ggatttatca aggcaacgtt
     5521 tcaatcctac ctcatggcga atacttagat ttaacgaaca acctcatcta tcctaacttc
     5581 agctaaataa atgacccgca tctgcaaatc tgtttatttg aaaccgcctg cggtcgccgt
     5641 tgatagactt acttacgtcc cagccatcag ggtcaatgtc aaaatcaaat attgccgtat
     5701 taggtattct taggttccat acccggttgg ttccaagaat taccgctatc aaatgaacta
     5761 agaatccact atgtgagtag gcaattacaa aatcttttga gttatggcct ttaacaagga
     5821 accctataac tgtctccgct ctttgcctaa tctgataacg cgtttcggct tttggtacta
     5881 aatcgaaatt tctcgatctg cgaaattctt caaatattgc agaatattcc gtcgataact
     5941 catcagatgt tttccctgaa aatactccag cgtcgttttc tctcagatct tgaattttat
     6001 gaacttgagc agcgggcaaa gagatttcgg cagttttatg agcccttgtt aatggactag
     6061 agtaaacatg agaaggttcc acttcctcct taacgaatct ttcacgaagc ttttcacatt
     6121 ggccttgacc ttctttcgaa agatcaaaat ccatagtccc ctgccaccga ccctcagcat
     6181 tagccttgga ttgtgcatgc ctgactaaaa ttaatctcat ttttttattc ctaatggcgg
     6241 gagagtgtcc gatttaagct ggttagcctg ttttctaagt ctatcgaaaa tcccgtcggc
     6301 aacaaccaaa agccccgcac atatcatcaa aatagtagac aattgaaatg ccttcgccat
     6361 tgctgacgca tatgcagttc gcaaaccgat atctttaagt ttctcggggt ccgataaatc
     6421 tgcttcatac cccaacccag ccattacaga aactacgatg gcggttggta ttgcaattcc
     6481 tacgatatga gccccatttc tagttaaatt taaaaatgca gacacaatac catagtgttt
     6541 cctaccagca ctccccataa tcgcactcgt gttaggagcc ataaaaatac tcattcccat
     6601 gccagacaac cctgacgcta tccctaccaa ggcaagggtg gaagtcaggc taacttcaga
     6661 gaacaaatac attgccacag cacaaaaagc cattccgata agtgatggac ctgtggtacc
     6721 aatcctgtca gctaaccgcc cactaagggg accaaaaata gccattaaaa tggaccctgg
     6781 tagtaaatac agtgctgcag tagcagttgt taatcctaga cctgagacca gaaaaaatgg
     6841 cattaagaaa aacgtgctcg agcttgcagt gaaagatata aatcttgacg aggagcccaa
     6901 agaaaaggaa agagatttaa atagactaag gggcagtatt gggtttttgg ctgttttttc
     6961 tctaaaaagg aacgctgcga acaacaatat agaacacata gttcccaaaa taatataggg
     7021 agaggacagt gataatttat tgctaaaagt tataaccaac attagtgaga gcaggaatag
     7081 cgctgaaatg aaagttccgc tccaatcaaa attcttgatg ggatctgctt tcccagggct
     7141 tttctgaggg tccggcgtca aaattaacat agcaatacac aatgcaaaaa tgctgaacac
     7201 tgctataaaa ataaaaatgg ccctccaccc aaaataacta tctatgctgc cacctaatac
     7261 agggccccca atagctccaa gaccaacgat ggtcatatgt aaccctagag ctttacctct
     7321 ctcttcgtca ggaaaagaac ccgcaatcat tgccatgcca ttcgcctgta aggctgaagc
     7381 accaataccc ataaaaattt tacctactat caccatccaa aattcttgag aaaaccctgt
     7441 gatagctgta gcacaagcaa atatcgacag cccaaatatc catatccttt ttctccctat
     7501 ggaatcagca atagcacctg ccggcatcat taaggcactt acactcaaca tgtaggcaat
     7561 tgccatccac tgagctgtgg gaatatctgc cccaaaatgt tccgcaatac tagggactac
     7621 tatagaagtc gcagactgtt caacaatgga tatgaaagtg ccaatggcaa ctgccccgta
     7681 aatccaccat cgatatgagg gtttatcttg gattcgactt aaaaaggaaa tcatcaatcc
     7741 tatttcttac tgtgctccaa tttgggtaat gcggatttcc cccttgtcat cagcgacaaa
     7801 tagactttca aaatcatacc ctctgcgttt gagttcctcg ctgccaccag ccattctatc
     7861 cattattgag agtactttaa caacctcaca accttcatct tcaaccgctt ctatggcgtg
     7921 aaacaaactg cctccagttg tgcatgtatc gtcaaccacg gcaacctttt gcccttcata
     7981 aaaatgtcct tcaatatttc taccgccacc atgagttttt ttctccttcc gtacgatcaa
     8041 agccggcaca ggttttcctt cgaggtagct taaagtacta acggaggaaa caatcgggtc
     8101 agctcctaaa gtaggtcccg ccaaagcctc tgcactacat ttgtacagga taggcaaaaa
     8161 acacttagct gtgagatatg atccttccgg atccagagtt aacagcctac catcaaagta
     8221 gtaactgctt ttattccctg atgacaggat gaattcgcca aatagcagag cctttctctc
     8281 tttggctact tctaataatc tatcaatatc attcattaca ataacagaat atatcccaca
     8341 atgttaattt aggccgttca atggtaaagc ttttgctcct taatgtattc atttacttcc
     8401 tgaggaatta agtcggctac atccacacca tcttgaataa ggtttctcac cctagtggaa
     8461 gaaatctcgg gcgagttaac ttcgatcgtt cttagtgaat attttctttg cctggacaga
     8521 tacgatttcg atttatcgat ttgaaaccca ggacgggtta atccaacaac ttcacattcg
     8581 gagtgaagta aatctggatc ctgccattca tccatttcaa ttattgagtc catcccaagt
     8641 aaaaacaatt acttcgtgac cctctcgctt taattctctt accgtgtcta tagtgtagga
     8701 aggtccaggc cgaaaaatct caatatctga tacttccaaa gaaggaattt ttcgaactgc
     8761 caaggacacc atacggtatc tttcttttgg cgatgccact aatttggttc tttttaaata
     8821 aggatctcct gcagggacca taattacttt atccaatttg agaataagat gagcagaatt
     8881 agcagcttca atatgaccat tatgtatggg gtcaaaagtt cccccgaaga gcccgacgaa
     8941 cactacaaac tcctgatgaa ttaactatgt ctctatttgc tagtaatttt acgacagcct
     9001 acagattaat gttgtgaatt cctgatactc tacaaccaac taacactcga acttgaggca
     9061 ttttacttga tgaagtacga ctatttagtg ataggagcag gttctgccgg agcgatagtg
     9121 gccgctcgac ttttagaaaa ccctgaaaac tctgttttgc tcctagaagg aggacctgac
     9181 tacccaatat ttgaagatct gccagatgac gtcaaatacg ggtatgccac aggcactgat
     9241 ctagctgtcg cagatgagca cgattgggga tacactgcca cagtaagcac ccaaagcgaa
     9301 actgctaatc gaatgattcg actgcccaga ggtaaattaa caggcggcac aagttcaata
     9361 aacggacaaa tatttcttcg cggactaact catgatttcc aaaactggtc tgaaaatggg
     9421 cttcacagct ggaattacca aaaagtttaa cttatttcag aaaactagag acagctatgg
     9481 attttggtgg ggattttcat ggaaaaaatg gtccaatctt agctaggcgg tttcccagag
     9541 atcaatggac cgcaccccaa gaaggatttt atcaagcatg catcgacagg gattttggca
     9601 aagccacaga tttcaatctt cctaacgcaa caggggtagg tgcatttcca tgcaacaatc
     9661 caaatggcat ccgcgtcagt acctcggttg gatatctgac tcaagcaagg catcggataa
     9721 atttcacact gcgttcaaat tgcacggtaa caaggctcgt agtagaaaaa aacaagattt
     9781 cagccgttga aatggaaagt gggggagata tgtttagggt ggaagcagaa actgtgatac
     9841 tttctgcagg aagtttggcc aaccctaaaa tattaatgct atctggaata ggtcccgcag
     9901 acaatctaac atcacatgga ataaaaccct tgataaatct agagggggtt gggaggaatc
     9961 ttccagatca cccacagaac tttgtgctag ctaaagtaaa aaatatagaa tctttggatc
    10021 ccaaatctcc acggctacag gctggtttaa gatacacatc tcaaaattcg gcccacacag
    10081 acgatatgat gatgtggatg ggttcttgtg cagtaagcgg agattataga gaaatattgt
    10141 cttcgaacaa atctcacaac caagttcaac cacagctagc agggatacag atcaccgttt
    10201 ctctttacct cggg
//
LOCUS       DASCNS010000114         4326 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000982874, whole genome shotgun sequence.
ACCESSION   DASCNS010000114 DASCNS010000000
VERSION     DASCNS010000114.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000982874"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="QF838_04085"
     CDS             complement(<1..222)
                     /locus_tag="QF838_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59902.1"
                     /translation="MDLNQLVNELIEVSKSGTRVPGFRGKTMIDADRLGILLSELQNS
                     MPSGVQEAQTIITQKDSIISQAQMEASRIV"
     gene            complement(257..730)
                     /gene="coaD"
                     /locus_tag="QF838_04090"
     CDS             complement(257..730)
                     /gene="coaD"
                     /locus_tag="QF838_04090"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HJO59903.1"
                     /translation="MAKAFYPGSFDPAHNGHIDVARRASRLFDQVIVGVYKAPPKNLM
                     FDTEERVALFQESLDGITNVEVVQFTGLAPTVAREVGAEVILRGLRAGFDFEQEFEMA
                     LMWRNLAPDIDVVCMMSALEHQFIYSSRIKEVARLGANIDNLVPPHVAEALKERI"
     gene            complement(723..1277)
                     /gene="rsmD"
                     /locus_tag="QF838_04095"
     CDS             complement(723..1277)
                     /gene="rsmD"
                     /locus_tag="QF838_04095"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HJO59904.1"
                     /translation="MIKISGGYLKGRSIGSPTKGKGVRPTTQKVKLALFSMLGDTVTD
                     SRVLDLYSCTGALGIEAISRGAVKCDFVEKVGRNCRLISNNLKIMDLDKVGKVHQSES
                     YRFVEQSEGGYDMVFLDPPFELDDWDRVMCEVGKSGFVNDGGKVVAEHRSNLDLDSFY
                     GNMNRINNKSYGDSKITIYEVVSG"
     gene            1495..2988
                     /gene="lysS"
                     /locus_tag="QF838_04100"
     CDS             1495..2988
                     /gene="lysS"
                     /locus_tag="QF838_04100"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HJO59905.1"
                     /translation="MSQQNEQEFMKVRIEKTASLRQKGIDPYPTNYKRTHTSKKAEEA
                     FESAEKSNTEFNKIIKVAGRIMGRRGMGKASFIDLSDTDGRIQIIMRSNVLNERYEIL
                     NDLDIGDWIGAEGTLFRTRTGQITLQVESFSILCKSLRPLPEKWHGLTDLETRFRQRY
                     LDLISNEDAVKTAKKRSLLVSTIRQFMSNRGFIEVETPMLVPIAAGGMAHPFTTHHNA
                     LNRDLFLRIATELHLKRLIVGGIEKVFEIGRVFRNEGVDLQHNPEFTTMESYEAFSDY
                     NDVMVMVEQLVSTAAKTLNGSETVTYGDEELKFSPPWPRIDLREKIIDVSGIDFFEHS
                     DVDSLKTAMGKAGVDVSQQVSWSGLLDKLISDKVEPTLVQPCFLVNYPVAMSPLAKKS
                     PTDERIAERFEGFVCGMEICNAFTELNDPIDQRARFEEQEMLRQEFQDEEMDRLDEDF
                     LVAIEHGMPPTGGLGIGIDRLTMLLTNNLSIREVILFPQLRSDRVEG"
     gene            2996..>4326
                     /locus_tag="QF838_04105"
     CDS             2996..>4326
                     /locus_tag="QF838_04105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJO59906.1"
                     /translation="MSDYTADLIIINANIHTVNSKVPHAQSFAVKNGRFVSVGTNSDT
                     QMLSGPNTEILDLTGYTVVPGFIDAHIHVLSSGSRHIMAADCDLRSISDVQDALKRRS
                     EKTESGNWVQGFKYDDTKTEENRFLTTSDLDKVSTELPIFVSHRAGHVYYANSKALSL
                     AGYTDDSDDPHGGRLGRDPNTGKLNGVIYERAVEAFRELLPTVDAEDRRRGLKLINGM
                     LSEVGLTSVHDASVSNDEFVTYQEGLENGDLDLRVYMLMAKAHFPALRDAGIRTGFGN
                     DRLKVGGIKMVSDGAIAARTAYLSQPYIGSDYDDCGIQAMSREEISESVKEMHSEGFQ
                     VCIHANGDLAISMVLDAYEEALKKFPRANPRHRIEHCTLVNPEILTRMNKTGTIATPF
                     CTYVYYHGEKMQFYGDERLEWMFAQKSFMEYGVTSTGATDYPPGPYEPLLG"
BASE COUNT         1346 a          932 c          890 g         1158 t
ORIGIN      
        1 aacaattcta gatgcttcca tctgtgcctg actgataatg ctgtcctttt gcgtaattat
       61 agtttgagcc tcttgcactc ctgatggcat actattttgt aactcagaca gaagaattcc
      121 tagccgatct gcatctatca tcgttttccc acgaaaaccc ggaacacgtg taccactttt
      181 acttacttca attagttcat tgacaagttg gttaagatcc ataattagta gcctccctga
      241 cttatactaa gcaaatctaa atacgctctt taagtgcttc cgcaacatga ggaggcacta
      301 aattatctat attggcccct aagcgggcaa cttcttttat cctactggaa tatatgaact
      361 ggtgctccaa tgcactcatc atgcacacca catcaatatc cggcgccaaa tttcgccaca
      421 tgagagccat ttcaaactct tgttcaaaat cgaatccagc ccttaaacct cgtagtatca
      481 cctctgcccc aacctcacgg gccactgtag gtgctaagcc tgtaaattga actacttcta
      541 cgttagttat tccgtccaaa gactcttgaa ataaggcaac tctttcttct gtatcaaaca
      601 ttaaattctt cggaggagcc ttgtagactc ccactatcac ttgatcaaag agacgggatg
      661 ctcgcctagc aacatcaata tgtccattat gggccggatc gaaacttcct gggtaaaatg
      721 ctttagccac ttacaacctc gtatatagtt attttagaat ccccatagct tttgttgtta
      781 attctattca tattaccgta aaaagagtct aaatccaggt tggatctatg ctctgccacc
      841 accttgcctc catcattgac gaacccagac tttccaactt cacacatcac cctatcccaa
      901 tcatcaagtt caaagggggg gtccaaaaag accatgtcat atcccccctc cgattgctca
      961 acaaacctat aagattcgct ttgatgcacc tttcccactt tatccagatc cataattttc
     1021 aaattatttg atatcaacct gcaatttcgt ccaacttttt caacaaaatc acatttcacg
     1081 gctccacgac ttatggcttc tattcccaga gccccagtac aggaataaag atccagcact
     1141 ctactatccg tcacagtatc tcccaacatt gagaagagtg ccaatttcac cttttgcgtg
     1201 gttggcctga cacccttacc tttggtggga gaacctatgg acctcccttt taggtaacct
     1261 cccgatatct ttatcaccta gacggatcct taagtcatct aaaattgaac ccactcactg
     1321 gatggtaaca attcacaact agttcgcaaa gcaaaatatg tgctttttta gaggcaatat
     1381 ttgtaagaaa ttttcagacc tctaatattt aatcaatttt atgttttaca tgtacttttg
     1441 gcgcagttaa aattagtttt caagttaaaa cattttagga aaaaggggtc agaattgtcc
     1501 caacaaaacg aacaagaatt tatgaaagtg aggattgaga aaacagcttc ccttcgacaa
     1561 aaaggtatag acccataccc aaccaattat aaaaggacac atactagtaa aaaagccgag
     1621 gaagcattcg aatcagctga aaaatcgaat acggaattca ataaaattat aaaagtagct
     1681 ggcagaataa tggggcgccg aggaatgggg aaagcttctt tcatagacct ttccgacaca
     1741 gacggccgga tacaaattat aatgcgaagc aacgttctca atgagcgcta cgaaatatta
     1801 aatgacctag atataggaga ctggattggg gcagaaggca ctttatttcg gacaagaact
     1861 ggccaaatca ctcttcaagt ggaaagtttc tcgatcctat gcaaatcact aagaccactt
     1921 ccagaaaaat ggcatggcct gacagactta gaaacaaggt ttcgccagcg gtatttggat
     1981 ttaatttcaa atgaggatgc agttaaaact gccaagaagc gtagtttatt agtcagcacc
     2041 attaggcaat ttatgtcaaa caggggcttt atcgaagtag aaactccgat gttagtcccg
     2101 attgcagctg ggggtatggc acatccgttt acaactcacc acaacgcttt aaatagagat
     2161 cttttcctga ggattgcaac agaactacat ttgaagcgcc tgatcgttgg aggtatcgaa
     2221 aaggtttttg aaatcggtcg cgttttcaga aatgagggag tagatctaca acacaaccct
     2281 gagttcacaa ctatggaaag ctatgaagca ttttctgact ataatgatgt tatggtgatg
     2341 gtagaacaat tagtctccac agctgctaaa actttgaatg ggtcggaaac agtcacatat
     2401 ggggatgaag aattaaaatt ctctcctcct tggccacgaa ttgacctgcg cgaaaaaata
     2461 attgacgtta gcggaataga cttctttgaa cactcagacg ttgattctct taaaacagcg
     2521 atgggtaaag ctggtgtgga cgtaagtcaa caagttagtt ggagtggcct tcttgataaa
     2581 ttgatctcag acaaggtaga acccaccttg gtccagccat gtttcctagt gaattacccg
     2641 gtggccatgt cacctttagc aaaaaaatcg cctaccgatg aaagaatcgc cgaaagattt
     2701 gaaggattcg tatgtggcat ggaaatatgt aatgccttca cagaactgaa tgatccaatt
     2761 gaccagcgag cgaggtttga agaacaagag atgcttcgac aagagttcca agacgaggaa
     2821 atggacagac tagatgaaga tttccttgtc gctatcgaac acggtatgcc acccacgggg
     2881 gggcttggca ttggtattga caggcttacg atgcttctca caaacaacct atctattaga
     2941 gaagtaatcc tgtttcctca attaagaagt gaccgagtgg aaggttgata aaaaaatgtc
     3001 tgattacaca gccgatctca taatcattaa cgcaaacata catactgtaa atagtaaggt
     3061 tccacatgcg cagagttttg ccgtaaagaa cggtagattt gtatcagttg gtacaaattc
     3121 agatactcaa atgctaagcg gtccaaatac tgaaatattg gatttgacag ggtatacagt
     3181 tgtacctggt ttcattgatg ctcatataca tgtcctcagt agcggttctc gccacatcat
     3241 ggcagcagat tgcgatctac gtagcatctc agacgttcag gacgctctaa aaagacggtc
     3301 agaaaaaact gagtctggta actgggtaca ggggttcaaa tatgacgata ctaagactga
     3361 agaaaataga tttttaacaa cttcagactt agacaaagtc agcacggaat tacctatatt
     3421 tgtgtctcac agagcgggcc atgtttacta cgccaacagt aaagctctat ctcttgcagg
     3481 atataccgat gattcggacg atcctcatgg ggggagattg ggaagagacc caaatactgg
     3541 aaaactaaat ggagtgattt atgaaagggc ggtggaggca ttcagagaat tactacctac
     3601 tgtagacgct gaagaccgac gcaggggact caaattaata aatggaatgc tcagcgaagt
     3661 aggattaact agcgttcatg atgcaagcgt atccaacgat gaattcgtaa cctaccagga
     3721 aggtctagaa aatggagact tggatctcag ggtttacatg cttatggcca aagcccactt
     3781 ccctgccctc agggatgctg gaatacgaac agggttcgga aacgatcgct taaaagttgg
     3841 tggcatcaaa atggtttctg atggggcgat agcagcgagg accgcctacc tgtctcaacc
     3901 atacataggt tccgactatg atgattgtgg catacaagca atgagtcggg aagagataag
     3961 tgaatctgtt aaagaaatgc attcggaagg ctttcaggtg tgcatacatg ctaatggaga
     4021 ccttgcaata tctatggtat tagacgcgta tgaggaagca ttgaagaagt ttccaagagc
     4081 taacccaagg caccgaatag agcactgtac ccttgttaat ccagaaattt taacgcgaat
     4141 gaacaaaacg ggaaccatag ccaccccttt ctgcacttac gtttattatc atggagaaaa
     4201 aatgcagttt tacggtgatg aaaggttaga gtggatgttc gcacaaaaat ctttcatgga
     4261 atatggcgtt acgtccaccg gtgctactga ttaccctcca ggaccgtatg agccgttatt
     4321 aggtat
//
LOCUS       DASCNS010000115        11680 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000996865, whole genome shotgun sequence.
ACCESSION   DASCNS010000115 DASCNS010000000
VERSION     DASCNS010000115.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000996865"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            173..1057
                     /locus_tag="QF838_04110"
     CDS             173..1057
                     /locus_tag="QF838_04110"
                     /inference="COORDINATES: protein motif:HMM:NF021287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HJO59907.1"
                     /translation="MDGLIVHDTPNTKLSTMVVAFAGWPDAAEAATRAIRYMVRKLPS
                     KKIAEMDPENYYDFTVNRPQTRINRRKDRIIKWPTNEFYSYVPEDNPENGLLMYVGTE
                     PNLKWRTFSDTVLEVAKLSGVELIISLGALLDAVPHTREVKITGRASSKELSKKARWL
                     GIKNSGYQGPTGIHSAFAEACAKNHIPHANIWGHCPHYVQTSPNPVTSFALLERLKNL
                     VDIDLDMSELELAGQAFREEISNAIAKQPDVSSYVSRLEHQYDEAKSSQNEMPTGTDM
                     VKEIESFLKSQSSGQEQE"
     gene            1087..1434
                     /locus_tag="QF838_04115"
     CDS             1087..1434
                     /locus_tag="QF838_04115"
                     /inference="COORDINATES: protein motif:HMM:NF021386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HJO59908.1"
                     /translation="MPHLSIDPDYYRTLFDRWTNDIAMLPDFPTDLKEKLVALHFIML
                     AFADGEEYSENEIHEGIKDRNLFSVDHVQIRINLLRQGFIVRIEKESEFIYKTSKEFL
                     THAQWDSSIPGAR"
     gene            1488..2321
                     /locus_tag="QF838_04120"
     CDS             1488..2321
                     /locus_tag="QF838_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59909.1"
                     /translation="MNCHDWGGQGRDIILVHGLASNYRIWDLVGPLLAETNSVKAIDQ
                     RGHGLSSKPNQGYDFDTVTNDLYLFLEANQIDKPLLVGHSWGGSVALNFAANHPEMVS
                     GLGLVDGGLIEISSIPGNSLEKALVDMAPPLFDSLREEFLRQRIAQRDWGERDSLSLQ
                     NDLANIVMANFQETQDGTITPRFERSNHLKVVEAFWNHHPSSLFSSIKCPVLNMPARL
                     NDDETSRQTLRKNLIVQAEKDIDKFKTVWLEHSIHDVPLQRPILVASTISSHLKEGFF
                     N"
     gene            complement(2318..2569)
                     /locus_tag="QF838_04125"
     CDS             complement(2318..2569)
                     /locus_tag="QF838_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59910.1"
                     /translation="MNQSIQLTLSLTSEAGRYATFQNGLNIDRGQEVLYLGNVSGGPI
                     TGSKGIVKKLYANKAVVDMGNVGTWNVPYYFLADTVKVA"
     gene            2734..3486
                     /locus_tag="QF838_04130"
     CDS             2734..3486
                     /locus_tag="QF838_04130"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO59911.1"
                     /translation="MGRLENKVALISGAAKGQGAFEAALFAKEGAKVVLADIDIDSCE
                     SNARHIVESGGEAVGIYLDVTSPASWEDCIKSTIKKYRSLTVLVNNAGIYSRVPIEEA
                     SSEEFDLIIAVNLKGVFLGTKYSIPEMRQSGGGSIINISSTAGLVGNQGGGAYGASKG
                     GVRLFTKFTAIQHASDNIRANSVHPGPIDTDMIADNISTPEGRENSISRIPMGRIGTI
                     EDVAMGVLFLASDESSYMTGSELVIDGGITAQ"
     gene            3556..4902
                     /gene="glmU"
                     /locus_tag="QF838_04135"
     CDS             3556..4902
                     /gene="glmU"
                     /locus_tag="QF838_04135"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HJO59912.1"
                     /translation="MHDFKAIILAAGAGTRMKSSLPKVLHKLSGKPMITLVMDSIVNA
                     GVQDITAVVPSCNEPFKEALEEKIEITVQKHQKGSGHALLQCRQKASNIENILVINGD
                     MPLIKAKTLSDLMAQHKSSKADLTLLTGKVTDPKDFGRIVRNNEGEVTKVVEESEANE
                     NQLLINEINAGAYCFKTAWLWAALESLKPSKSGEFFLTDLISFAESINLSIESISADN
                     QEIMGVNNKSDLAKAEKILRIITCENLMETGVRIIDPDTTYIDLGVLISPDTVIFPNT
                     YISGNTNISSNCEIGPNCEITNSSLGSSCIIKSSTIEFAMIRDNVSIGPYSHIREGSI
                     LNEDVSIGNYVEIKNSTLGRGTKSGHHCYLGDSDIGSNVNIGAGTITCNYDGEQKHRT
                     TIRDDAFIGSDTKLVAPVTIGKQSVTGAGSIVTKDIPDFAKGVGSPARIVKEDRSS"
     gene            4915..6171
                     /locus_tag="QF838_04140"
     CDS             4915..6171
                     /locus_tag="QF838_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015436.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HJO59913.1"
                     /translation="MDTDISDLSSLTLIVSIALFTLISFVQHVIGTVTRESIFIWRSE
                     ERSGLEKLEDLFRKADQSNSAFSIIKTISLASTVINCYFIFYQPSNNQPTSYDFGQIS
                     LIAISTLIVLGILETVAKVAGRHSGPRFAVRIYRIAIFIGTTLRPITRSQERLIEIST
                     VEENSSGISENSNIAMQMDDEGEPLEEHEVRMIRGVFQLDKTIVREIMVPRVDMITAD
                     ISTPINDVVDIMIREGHSKIPIYKNETDQIEGIAHSMDILGQIRSAESNNGSELKEFL
                     RPVLFIPESKTLEFLLTDFQDKRMQMAIVIDEYGGVSGLVTVEDLVEEIVGELHDEFD
                     QRELGIREVETNEFYMDASIAIDDIALSIGVSFEGEGFDTIGGFVLHELGKIPSPGDN
                     FSYNNLSIEVISTVGRRLREIKILKS"
     gene            complement(6537..7388)
                     /locus_tag="QF838_04145"
     CDS             complement(6537..7388)
                     /locus_tag="QF838_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HJO59914.1"
                     /translation="MGKLFSLQALLTALVFSVMLFVLSACAAPAAGQPGLPGNPGSPG
                     ISGAQGPQGEPGLPGLPGNPGPAGAPGLQGPAGPDGSDAVAPEGNIAVSKSRVTMSEA
                     FSVSGSGFQPNEPVVIQLRIDSSLSPIIGGGRGSQVTANGAGAFEVSFDFVSEKAAII
                     GRAGGPSTVFAQGGSGSKASAPITIVSSSSPTGNASASLAATPAEAGGTSIVYGAGFA
                     PGGMVSIIAVGAADGVDKILAGGEVNASGAFQIDVKVALDAGLYTLTAKGSSNSEATA
                     PLLVADK"
     gene            7752..8951
                     /locus_tag="QF838_04150"
     CDS             7752..8951
                     /locus_tag="QF838_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013832.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HJO59915.1"
                     /translation="MTLDNKNSHSASWILLAGLACLGMPSFIMVFGGIHFHPILDAFI
                     FGMGIVGGAFLISWAAEAAQLDVSASFAIAILALIAILPEYAVEAVLAWDAGQSYDAA
                     SGTITVEMERVAANVTGANRLLVGLGWSAEILIFWLKRRTSLNMKGAMGLEILMLSVA
                     TAVTFLIFFMQQVHVIVAVALISLYLVYLWLSSTKESEEPELMGPSLAIGEQSKLTRR
                     LIVLALFAYSAFVILVAAEPFVHALVETGRDFGISDFLLIQWIAPLASESPEIIIAVL
                     FSLRANPVAGLTTLISAEVNQLTLLVGSMVGIFSLSVGEILSFPLNNMQSVEFLLTAA
                     VSVLAIILVLPRIIGWKSGMILLVLFLAHLPFTETDQRLLFTYGYLAIAAVLIVYQLY
                     RKYLSKN"
     gene            complement(8972..9844)
                     /locus_tag="QF838_04155"
     CDS             complement(8972..9844)
                     /locus_tag="QF838_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HJO59916.1"
                     /translation="MKVHLVDGTYELFRAHFGQPERLAPDGMHVSAVRGLIMTLIALL
                     NQPEVTHIAVAFDSSIKSFRNELLPTYKDGSDTPLELKMQFPLAERVVNALGIVSWPM
                     HDYEADDALATAALLYSKQDTVQQVIICSPDKDLCQMVDGDQIVCLDRKNNLILNELG
                     VLEKFGILPISIPDYLGLVGDTADNIPGIPKWGAKSSSTLLNRYIHIEDIPNTYELWD
                     VSVRGAAALSLSLEDSREEACLYREIATVRTDLEITETLKELEWLGASKNLFPSLCRE
                     LGLGNLSNRPQKWQ"
     gene            complement(9874..10812)
                     /locus_tag="QF838_04160"
     CDS             complement(9874..10812)
                     /locus_tag="QF838_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59917.1"
                     /translation="METAISNKGHLSRAFTRLWHKKIAVVCLFAILLIYFMGIFAPLI
                     APYGYNEQDYQSIREGPTMEHIAGTDLKGRDVFSRVLWGIQNTVIITLVTILTGGLLI
                     GVSLGLISGYYGGRVDSVIMRIGELFASFPDILLVILLAATLRPRILNVVRWIEDNTL
                     LDGLVKSGVADYLVISLALVSFSWVGMARLVRGQVLSLKSSQYVESAQATGASTSRIL
                     FIHLLPNAIGPIVVMVSMGMGTLIGTEIILSWLGLGIQPPRPSLGTMLLEAGSISALR
                     VVPWMLIAPGVVAWSLVLCWNLFGDAMSDVLNPSTR"
     gene            complement(10812..>11680)
                     /locus_tag="QF838_04165"
     CDS             complement(10812..>11680)
                     /locus_tag="QF838_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO59918.1"
                     /translation="VTFVLGTYGPGDPVRVMLGTKYDEETADRLRSSLGLDRPVIVQY
                     ADYISGAIRGDFGESLRYRGRSVGSLLASKMWVSFQVNLAAMVVSLSIGLPLGFFIAH
                     KQGTWVDPAAVAFTLILMSIPIMVSIPIVLWGLCLKLSLVPCSGWGGFFDLRIIVPAI
                     TMGIPGVAGLARLMRASTLDVFGQDFIRTARAKGLGERYIDVRHVLRNAIIPVVTILS
                     FSLAGMLTTGFITERLLGIPGVGDFAIQAIFNRDYPVIMAFTLILSSAFVVANLLADI
                     AYSLIDPRIRLS"
BASE COUNT         3668 a         2511 c         2383 g         3118 t
ORIGIN      
        1 gtcatatggt ctgctcctta ccaaaattct taccattttt agttttcaag tgtatacgga
       61 attaatagat tcacttgtca taagcacaat atcaggggat cacaaaacaa ttgttggatt
      121 atatggtgta aagtgttaaa ctcggataaa ttcaacaaca acggagcagc atatggacgg
      181 cttgatcgtt cacgataccc ctaacacaaa gctttctact atggtggtag ctttcgcagg
      241 atggccagat gctgctgaag cagccacaag ggccataaga tacatggtgc gaaagttacc
      301 aagtaaaaag atcgctgaga tggatcctga aaattattat gatttcacag taaaccggcc
      361 ccaaactcgg attaacaggc ggaaagaccg gattataaag tggcctacaa acgaattcta
      421 cagctatgtc ccagaggata acccagaaaa tgggcttctt atgtatgtgg gaaccgaacc
      481 gaaccttaaa tggagaactt tttcggatac cgttctagag gtagcgaaat tatctggggt
      541 tgagctaatc atttcgttag gcgccttact ggatgcggta ccgcacacta gggaagttaa
      601 aataactggc agagctagca gtaaggaact aagtaaaaag gccagatggt taggaataaa
      661 aaattctggt tatcaaggac ccaccggaat ccattctgct ttcgctgaag catgtgcgaa
      721 aaatcacatt cctcatgcaa atatttgggg ccactgccct cattatgtac aaacctctcc
      781 aaatcctgtt actagttttg ccttgctgga acgtcttaaa aaccttgtgg acatcgattt
      841 agatatgagt gaactagaat tagctgggca agctttcagg gaagaaatct ccaatgctat
      901 tgcaaaacaa cctgatgtga gctcatatgt atctcgtctt gaacatcaat atgatgaagc
      961 aaaaagttct caaaatgaga tgccaactgg aactgatatg gtgaaggaga ttgaaagttt
     1021 cctaaaaagt cagtccagtg gccaggaaca agaataagaa gattcctaat ggcggatatc
     1081 caataaatgc cgcatttatc aatagaccca gactattacc ggacactttt cgatcgttgg
     1141 acaaatgaca ttgcaatgct ccctgatttt ccaaccgacc taaaagaaaa attagtcgcc
     1201 cttcatttca taatgctggc ttttgctgac ggtgaggaat attcagaaaa cgagatccat
     1261 gaaggaataa aagacagaaa tctcttttct gtcgaccatg ttcaaataag gattaacctt
     1321 ctccgacaag ggttcattgt tagaattgaa aaagaatctg aattcatata caaaacatcc
     1381 aaagagtttt tgacccacgc tcaatgggat agttcaatac ctggagcccg gtaataaacg
     1441 atcaacacag cccaaagcac aatttaataa atatcggacc aatatcgatg aattgccatg
     1501 actggggagg gcaaggtagg gatatcattt tagttcatgg gttggcctca aattaccgca
     1561 tttgggatct agttggcccg cttttagcag aaaccaattc agtaaaagca atagatcaaa
     1621 gaggccatgg attgtcttct aaaccaaatc aaggatacga cttcgatacg gtcacaaacg
     1681 atttatattt attcctagaa gccaatcaaa ttgataagcc tttgttagtc ggtcattcat
     1741 ggggaggcag tgttgcattg aactttgcgg cgaatcaccc tgaaatggta agtggtctcg
     1801 gccttgttga tggggggtta atcgaaattt cgtccatacc aggtaattct ttagaaaaag
     1861 cccttgtaga tatggcccct cctttatttg acagcctaag agaagaattt cttagacaaa
     1921 gaattgcaca aagagactgg ggggagcgtg atagtctctc cctccagaac gatctagcta
     1981 atattgtaat ggcaaacttt caggaaactc aggacgggac cataacacct cgtttcgaga
     2041 gaagtaatca cttgaaagtg gtggaagctt tctggaatca ccacccttcc tctttatttt
     2101 cctcaataaa atgccctgta ttaaacatgc cagcgagatt gaatgatgat gaaacttcca
     2161 gacaaacact tagaaaaaac ttaatagttc aggcagaaaa agatatagat aaattcaaaa
     2221 cagtttggtt agaacatagc atccatgatg tgccgcttca aagacccatc ttggtcgcat
     2281 ccacgatatc tagtcattta aaagaaggct tttttaacta agcaaccttg actgtatcag
     2341 ccaaaaaata atatggcaca ttccacgtcc cgacatttcc catatctaca acagctttat
     2401 tagcataaag cttttttacg atgcccttgc taccagtaat tggtccccca ctaacattcc
     2461 ctaaatacag aacctcttgg ccacggtcta tatttaggcc attttgaaaa gtagcatatc
     2521 ggccagcttc actggtcaga gataaagtaa gctgaattga ttgattcata gggacacctt
     2581 gaagaataat ccgatttaga acattttaga acagaacact tgttctgtca attgaagtcg
     2641 gtgaatatat ccaaataaca agattcagat gatataaaga ctccttctga gatagaatac
     2701 ctaataataa tcctgccata gaggtatagc atcatgggga gactggaaaa caaggtggca
     2761 ttaatttccg gagcagcgaa agggcaagga gcctttgagg cagctctttt tgcaaaagaa
     2821 ggtgccaaag tcgtattggc agatatagac atagactcat gtgaatcaaa tgcccggcat
     2881 atagtggaat caggaggtga agcggtcgga atatacctag atgtgacctc tcctgccagc
     2941 tgggaggatt gtatcaaaag tacaatcaag aaatatcgga gtttaacggt actggtaaat
     3001 aatgccggca tatatagtag ggtaccaatt gaagaggcat ctagtgagga atttgatttg
     3061 ataatagcag tgaacctcaa aggggttttt ctgggtacta aatattctat tcctgaaatg
     3121 cgtcaatccg gaggtggttc aattatcaac atatcctcta ccgctggatt agtgggaaat
     3181 caaggagggg gagcctatgg tgcatcaaaa ggcggggtta gactttttac caaatttacg
     3241 gcaatacaac atgcttccga taacataaga gctaattcgg ttcatccggg tccaatagat
     3301 acggacatga tcgcagataa tatatccact cctgagggta gagaaaactc catatctagg
     3361 atacctatgg gacgaatagg aactatagaa gacgtcgcga tgggcgtcct attcctcgcc
     3421 tctgatgaat cttcatatat gacaggatca gaattagtca tagacggagg aattacagct
     3481 cagtaaactt gtaataatta tgtatttgta ctgttggtga aatactgatt aatcaaattg
     3541 cctttaatga acataatgca tgacttcaaa gcgattatat tagcagccgg ggcgggaact
     3601 aggatgaaat ctagtctacc caaggtgttg cataagctgt ccggaaaacc aatgataacc
     3661 ttggttatgg attccatagt caatgctggg gtacaggaca tcactgctgt cgtaccatcc
     3721 tgcaatgaac catttaaaga agctttagag gaaaagatag agattacggt ccaaaaacac
     3781 caaaaaggaa gcggacacgc tctattacaa tgtagacaga aggcatccaa catcgagaat
     3841 atccttgtca taaatggtga tatgcctttg attaaagcaa aaactctttc cgaccttatg
     3901 gcccagcaca aatcgtctaa ggccgacctt actttactta ctggaaaggt caccgaccct
     3961 aaagatttcg gacgcatagt tagaaataat gaaggggaag ttaccaaggt agtcgaagaa
     4021 tccgaggcta atgaaaatca attacttatc aatgagatta atgcaggggc ctattgtttc
     4081 aaaactgcat ggctatgggc agccttggaa tccctaaaac catccaagtc tggggagttt
     4141 ttcctcactg accttatctc ttttgctgaa agcataaatt tatctataga atccattagt
     4201 gctgacaatc aggaaataat gggagtaaac aataaatcag atttggctaa agccgaaaag
     4261 atattgcgaa tcataacttg tgaaaacctg atggaaacag gagtaagaat tatagatcct
     4321 gatacgacat acattgacct aggagtattg attagtcctg acaccgtaat ttttcctaac
     4381 acctatatca gtggaaatac taatatctct tcaaattgcg aaataggccc aaattgcgaa
     4441 attacaaata gttcccttgg gtcctcttgt ataatcaaat catctaccat cgaattcgcc
     4501 atgatccgtg ataacgtatc cattgggcca tatagccaca ttagagaggg gtctattcta
     4561 aacgaggatg ttagcatagg taattatgtg gaaataaaaa acagtacgtt aggtcgaggt
     4621 acaaaatcag gtcaccattg ctatctcgga gactcagata taggttccaa tgttaatata
     4681 ggagcaggta ctataacctg caactacgat ggtgagcaaa aacatagaac caccataagg
     4741 gacgatgcct tcattggatc tgacacaaag ctggtcgcac cagtgaccat agggaaacaa
     4801 tctgttacag gagccggatc aatagtcaca aaggatatac ctgattttgc aaaaggagtc
     4861 ggttccccag ctcgtattgt aaaggaggat aggtcttcat aggcccaagc caaattggat
     4921 actgatataa gtgatttatc aagtttaacc cttatagttt cgatcgcact tttcacatta
     4981 ataagctttg ttcaacacgt aattggtacc gttaccagag aatctatatt catatggcgg
     5041 agtgaagaaa ggtccggttt agaaaaatta gaggaccttt tccgtaaagc tgaccagtca
     5101 aattctgcat tttcaatcat aaaaacaata tccctggcat ccacagtgat aaattgttat
     5161 ttcatatttt atcaaccttc aaataatcaa cctacatcct atgattttgg acaaataagc
     5221 ctcatagcta tatcgactct tatagtgcta gggatattag aaacagtagc aaaggttgcc
     5281 ggacgacaca gtggacctag gttcgcggtg cggatatata ggatagctat tttcatcggc
     5341 acaactttaa gaccaatcac acgttctcaa gaacgtctaa tcgagataag cactgttgag
     5401 gaaaattcat caggtatcag tgaaaacagc aatattgcaa tgcaaatgga cgacgaaggc
     5461 gaacctctgg aggaacatga agtacgaatg atacgaggcg tcttccaatt agataaaaca
     5521 atagtacggg aaattatggt tcctcgagta gatatgatca ctgccgatat atcaacgcct
     5581 ataaatgatg tggtggacat aatgatcaga gaaggccata gtaaaatacc aatctacaaa
     5641 aatgaaacgg accaaattga aggcatcgcc cattcaatgg atatactagg gcaaataaga
     5701 tctgccgaat caaataatgg tagtgaattg aaagaattcc ttcgcccggt gctgtttata
     5761 cctgaatcaa aaacactgga gtttttgtta actgactttc aagacaaacg tatgcagatg
     5821 gctatagtaa tagacgaata cggaggggta tcaggcctcg tcacagtaga agatctcgtg
     5881 gaagaaatcg tcggagagct tcatgacgag tttgatcaac gtgagttagg aataagggaa
     5941 gtggaaacaa atgagttcta catggacgct agtatagcca ttgatgacat agctctttct
     6001 ataggggttt cttttgaagg tgaagggttt gacactattg ggggattcgt attacacgaa
     6061 cttgggaaaa tacctagccc aggagataac ttttcctaca acaacctttc tatcgaagtg
     6121 atttctacag ttggccgtag actcagggaa ataaaaatac ttaaatccta acagaagtaa
     6181 aactaggcac cttttacagt gtctattctc cccattgtcc cttttactcg taggtatcct
     6241 ataaattgat tgttactgcc caaagcctat gcgacttgtg caataaccac aaatatccaa
     6301 cacctcgtag gtacaaaaag ctttgacgaa atgaatgtca ccactaatta tccctagttt
     6361 tgccttcagc aaacgtttct aacattcaaa ttcaaagaat tagttaccca ctgattgtgc
     6421 aaaaattcat ttttcgaaaa actaataaga cccccaaaca gttaagtttg agggccttag
     6481 ttcagccagg ttctatgtta tctaactaaa ttgattcagt taggaacctc gtttgcttat
     6541 ttatcagcta ctagtaatgg agctgtggct tctgagttag aggaaccttt ggcggtaagc
     6601 gtatagaggc ccgcatcaag agccacttta acgtcgattt ggaaggcacc ggaagcattt
     6661 acttctccac ctgccaatat tttatcaact ccatcagctg caccaacggc gatgatgcta
     6721 accatcccac caggtgcaaa tcctgcgccg taaacaatgg aagtaccacc agcctctgca
     6781 ggtgtcgctg ccaagctagc cgatgcgttc cccgttggag aagacgatga cactattgtt
     6841 ataggtgcac ttgctttact accacttccg ccttgcgcga acacagtaga aggaccacct
     6901 gccctaccta tgatagcggc cttttcagac acgaaatcaa aggaaacttc aaaggctcct
     6961 gcaccattgg cagttacttg agaacctctt ccgccaccaa taattggtga caaacttgag
     7021 tcgattctaa gttgtatcac gaccggctcg tttggctgaa agcccgaacc agaaacactg
     7081 aatgcctcag acatggttac cctagactta ctaacagcta tattaccttc tggtgccaca
     7141 gcgtcagaac catctggacc tgctggtcct tgaagaccag gagcgccggc tgggccagga
     7201 ttgccaggca aacctggaag gccaggctct ccttgcggtc cctgagcacc ggaaattccg
     7261 ggactaccag gattacctgg aagaccgggc tgtccagcag caggtgctgc acatgctgat
     7321 aatacaaata acattactga gaaaaccagg gctgttaaca acgcctgaag gctaaatagc
     7381 tttcccattt gacccccttc tttctataaa ggaacgaatt ttagtccaaa cttgtttgtt
     7441 gaaggagcaa gcagcaaaac agcaacaagc cccggcaacg gcgaatgaac ggtttcatga
     7501 tatgcaccac ccttaggtat gtcaagctat tcccgtgcct aattcaccac tctgaaacaa
     7561 ccatccaaac aaggggtcat aaaccgactc gtagtcactc cccgcccaac cctagaattt
     7621 tagttgaata actatgaata atcaacagtt gaaaatatac tatgctagaa tttcgcacta
     7681 ttgcacccta ctgtggttta tcgggggcat aatttggaaa gatctgattt agaatatatg
     7741 gtgatgataa agtgactctg gataataaaa atagtcattc agcctcttgg attctattgg
     7801 ctggactcgc ctgcctaggg atgccttctt tcataatggt attcggagga atccattttc
     7861 atccgatctt agacgccttc attttcggaa tgggcattgt ggggggagct ttcctcattt
     7921 catgggccgc cgaggccgcc cagctagatg tttcagcttc ttttgcaata gccatcctag
     7981 ctctaattgc aattctcccg gaatacgcag tggaagctgt tctagcatgg gacgcaggcc
     8041 aatcctatga tgctgcgagt ggaacaataa ctgttgaaat ggaaagagta gccgccaatg
     8101 taaccggagc aaacagatta ctcgttggtt tgggatggtc agccgaaatc ttaatttttt
     8161 ggctgaaaag aagaacctca ctaaatatga agggcgctat gggccttgag atactgatgt
     8221 tgtcggtggc aactgcagtt acatttttga tatttttcat gcagcaagta cacgttatag
     8281 tggctgtcgc cctaatatct ctttatctag tttatttgtg gttgagctca acgaaggaat
     8341 ctgaggaacc agaactaatg ggcccctcat tggccatagg agagcagtct aaattgacca
     8401 gaaggctgat tgttttagcg ctatttgcct attctgcttt tgtgatttta gtggctgcag
     8461 aaccatttgt tcatgctctt gtagaaactg gacgagattt cggaattagt gactttttat
     8521 taatccaatg gatcgctcca ttggcctcag aatcaccgga aataataatt gctgttctat
     8581 tcagccttcg agcaaaccct gtagcaggac taacaactct tatttctgca gaagtcaacc
     8641 aactcactct tttagttgga agcatggtag ggattttcag cctttctgta ggagaaattc
     8701 taagtttccc gctcaacaac atgcaatcag tggaattctt gttaactgca gctgtatcag
     8761 tgctagcaat tatattggtt ttgcctcgaa taatcgggtg gaaatcggga atgatattac
     8821 tagtactatt cctagcacat ctgccgttta ctgagacgga tcaaagatta ctttttacat
     8881 atggttattt agcaattgca gcagtcttaa ttgtctacca gttatacaga aaatatttgt
     8941 ctaaaaatta gtcagtaaat tcactttgat cctattgcca tttttggggc cggttcgaca
     9001 aattccccaa acctaattca cggcacaaag atggaaaaag attcttcgaa gcccccagcc
     9061 attccagctc tttaagggtt tccgtaatct ctaaatcggt acgcactgta gcgatttccc
     9121 gataaaggca ggcttcctct ctactgtctt caaggctcaa acttagagct gctgcccctc
     9181 ttacagagac atcccagagt tcgtaagtat ttggtatgtc ctctatgtga atataccgat
     9241 taagaagagt agagctagat ttcgcacccc atttcggaat gcctggaata ttatcggctg
     9301 tgtcaccgac taatcctaaa taatcgggta ttgatatcgg taaaatgccg aatttttcaa
     9361 ggacgcctaa ttcgttcagg attaaattat ttttcctgtc taggcatacg atttgatccc
     9421 catcaaccat ttgacataaa tccttgtcgg gtgaacaaat gattacctgc tgcacagtat
     9481 cctgttttga atagagcaat gctgctgtgg ccaatgcatc atcagcttca taatcatgca
     9541 taggccatga cactatacca agcgcattta ctactctttc agctaatgga aattgcattt
     9601 tcaactctaa aggggtgtcg gatccatcct tgtatgttgg tagcaactca tttctaaaag
     9661 atttgattga actatcaaag gccactgcta tgtgagtaac ttctggttga tttagtaatg
     9721 ctatcaaggt cattatcaaa cccctgacag ctgaaacatg cattccgtca ggtgctaatc
     9781 tttcaggttg cccaaaatgg gctcgaaata attcatatgt tccgtcgact aaatggactt
     9841 tcatatctca ctttatttaa agatttacca gattcaccta gtgctggggt ttaatacatc
     9901 actcatagca tcaccaaaca aattccagca aagcactaat gaccaagcta caacgcctgg
     9961 agcgatcaac atccaaggaa caacccgaag cgcgctaata ctacccgctt ccaataacat
    10021 agttcctaaa ctaggtcgcg gtggctgtat acccaaccca agccaactca atataatctc
    10081 ggttccaatc aaggtaccca tgcccatcga gaccataact acgataggac ctatcgcgtt
    10141 aggtagaaga tgaataaata aaatccgtga agtagaagcc cccgttgcct gggcggattc
    10201 gacatattgt gagcttttta aagataagac ttggccccgt actaaacgag ccatcccaac
    10261 ccaactaaat gaaaccaagg ccaaggagat cactaaatag tcagcgactc cagatttgac
    10321 tagcccatct aacaaggtat tatcttcaat ccaacgaaca acatttaaaa ttcttggcct
    10381 taaggtggca gccaataaaa taactaacaa tatgtccgga aaagaggcga aaagctcgcc
    10441 tatcctcatt attactgaat caaccctgcc tccatagtaa ccagaaatca atccaagcga
    10501 tacacctatc aacaatcccc cggttagaat cgttaccaag gttattatca ctgtgttttg
    10561 tatcccccac aggaccctcg aaaaaacatc cctacctttc agatcagtcc cagctatatg
    10621 ttccattgtc ggaccctctc tgatcgattg gtaatcctgt tcgttatagc cataaggtgc
    10681 tatcaaaggt gcaaatatac ccataaaata aatgagcaag atggcaaaca aacacacgac
    10741 tgctattttt ttatgccaca gcctagtgaa agcccgagac aaatgacctt tgttactgat
    10801 ggctgtctcc actaggaaag ccttatgcgt ggatcaatta gtgagtaggc gatgtcagcc
    10861 aacaagttag caactacaaa ggcacttgat agtatgagag tgaatgccat tattactgga
    10921 taatccctgt taaaaatagc ctgtatagca aaatctccta cacctggtat accaagtaat
    10981 ctctcagtta tgaacccggt ggttagcata ccagctaggg aaaaagacaa aatggttacc
    11041 acaggaatga ttgcatttct cagaacatgc cttacatcaa tatatctttc acctaaacct
    11101 tttgctcttg cagttctgat gaaatcttga ccaaatacat ctagggtact agcccgcatc
    11161 aaacgagcaa gtccagcaac cccaggaatc cccattgtga tagcaggaac tataatcctc
    11221 aagtcaaaaa atcctcccca ccccgagcat ggcaccagac tcaatttcaa gcacaaaccc
    11281 cacaaaacta taggaatgct caccataata ggtatggaca ttagaatcaa agtgaatgca
    11341 acagctgcag gatctaccca agtgccttgt ttatgggcta taaaaaatcc taaaggtaac
    11401 ccaatggaca aactgacaac cattgcagct aggttaactt gaaaagaaac ccacatcttg
    11461 gaagccaaca aagagcctac tgatcggccc cgatatctaa ggctttctcc gaagtctccc
    11521 ctgattgccc cagatatgta gtcagcgtat tgaactataa caggcctatc cagccccaat
    11581 gaagatctaa gtctgtctgc agtctcctcg tcgtatttag ttcctagcat caccctcaca
    11641 ggatcaccag gcccatatgt tccgagaacg aaggtaactg
//
LOCUS       DASCNS010000116         1246 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000000999967, whole genome shotgun sequence.
ACCESSION   DASCNS010000116 DASCNS010000000
VERSION     DASCNS010000116.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000999967"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="QF838_04170"
     CDS             <1..385
                     /locus_tag="QF838_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013079.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HJO59919.1"
                     /translation="TLTGAIRTALGDQIIGTFGNNDEFTKEVIEAGAREGERLWELPA
                     DESFSKHFKSDVADIKNVGGAAGGATIGAMIIGMFAEDTPWVHLDIAAVSRTNANNGD
                     DPKGATGSPIKTLVSLCKFISAYRY"
     gene            493..792
                     /locus_tag="QF838_04175"
     CDS             493..792
                     /locus_tag="QF838_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJO59920.1"
                     /translation="MEQDRLPKWTFITNHGLVLSYIFHNPTSTAREIANYIGVTERTT
                     HKIISDLEEAGYIRRRKIGRRNVYSVDPQLPLRHHTKTDVMVEDLLEALVSEAGI"
     gene            859..935
                     /locus_tag="QF838_04180"
     tRNA            859..935
                     /locus_tag="QF838_04180"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:His,seq:gtg)
     gene            990..>1246
                     /locus_tag="QF838_04185"
     CDS             990..>1246
                     /locus_tag="QF838_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59921.1"
                     /translation="MSKHNRISKILREGDSNGDGWSLELSSSTGRKIFAVAVAQNNRR
                     SRTGPTWSYVFENNGWTSIDLGTPGSYEALKNGYSTIGINPS"
BASE COUNT          377 a          255 c          293 g          321 t
ORIGIN      
        1 cactttaacc ggggcgataa gaaccgcgtt gggggaccaa attatcggta cttttggaaa
       61 caatgatgaa ttcactaaag aggtaataga agcaggggcc agggaagggg aaaggttgtg
      121 ggaattacct gctgatgaat ctttttctaa gcatttcaaa agtgacgttg cagacatcaa
      181 aaatgtcggc ggtgcagccg gtggggccac tatcggggca atgattatcg gaatgtttgc
      241 tgaagatacc ccttgggttc atctagatat tgccgctgta agcagaacta atgcgaataa
      301 cggcgatgat ccaaaaggag caacaggctc tcctatcaaa accttagtat ctctatgcaa
      361 atttatctca gcttatcgtt actaatcaac tacctaattt attaaagttg tcatgctgat
      421 gacaggtatg gtactgtcac ttacccgaac tcgatagatt aagaaaggga aacctttgtt
      481 gaggccttac atatggaaca agaccgacta ccaaagtgga ctttcataac caatcacgga
      541 cttgtcctga gctatatttt ccacaaccca actagtacag ctagagaaat agccaactat
      601 attggtgtga cagaaaggac tacgcataaa ataatatcgg acctggaaga ggccgggtat
      661 attcggaggc gtaagatagg tcgtaggaat gtatacagcg tggacccgca attgccactt
      721 cgacatcaca ctaaaacaga cgtaatggta gaagacttac tggaagctct tgtctctgaa
      781 gccggcatat agaatctaat tttgttcttc acaaattgac ccttcttcaa ataggatgct
      841 agacttttcg ctgctgtggt gagcgtggcc aagtcggtta aggcgccagg ttgtggccct
      901 ggagatcgag ggttcgaatc ccttcgctca ccccatcttc ataattacat atatgagcct
      961 cagtacgcct gaactggttg ggaatttaat tgagcaaaca caatagaata tcgaagattt
     1021 taagagaagg ggactctaac ggtgatgggt ggtcgcttga actcagttct tcaacaggaa
     1081 gaaaaatttt cgccgtagct gtcgcacaaa ataatcgccg tagccggaca ggaccaacgt
     1141 ggtcatatgt ttttgaaaat aacggatgga cttcaatcga tttaggtaca cctggatcct
     1201 atgaagcgtt gaaaaatggc tactctacca tcggtataaa tccatc
//
LOCUS       DASCNS010000117         2378 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001004977, whole genome shotgun sequence.
ACCESSION   DASCNS010000117 DASCNS010000000
VERSION     DASCNS010000117.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001004977"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="QF838_04190"
     CDS             <1..1475
                     /locus_tag="QF838_04190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJO59922.1"
                     /translation="TGGVDNIFPHHEGEIAQSEGYTGFQVVNHWVHGQHLLADGVKMA
                     KSAGNAFIIPDLVERGIDPLAFRYLCMTAKYRTRINFTFTSLKACETALNKLRRHYIL
                     FSKNSQNQTLNNETVKEWKKRLIGIVCEDLNMPGLVDGIWRLIESSDAADSDKAAILS
                     SIDNLMGLGLDSTDDMYDLPEKIIGKSDERTELRASLDYERADRLRTEMEELGYMVED
                     AKDYSTIRLKDSSEIREERWKTISSSSEIASKVFSESVVPVTVGVVVNGYPDDTQRCV
                     SSLIKWTESDQTEIIVIDNGSTDLTGELLEEMTKDQENLHILHADHTLGDGSAKNILL
                     KMAQGEIFVLLDPSVEIKGNFIKPIQGLLKDPEVGIAGLAGLKTEDLMHFHDGEGESG
                     QVDAMQGYCFALRREDLKKVGLMRESFRYYRNLDLDYSFQFKEQGLSIIADHTLPMEL
                     HEHRGWTALSEGERDELSKKNYGRFLKKWRGRTDLLISNK"
     gene            1568..>2378
                     /locus_tag="QF838_04195"
     CDS             1568..>2378
                     /locus_tag="QF838_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013832.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HJO59923.1"
                     /translation="MDSLSLPLLWVVLAGLAAIILISATQLTKNADIIAFKTGLGRSF
                     VGVVLLATATSLPELATGVSSVLSIGGAGGADLAAGDAFGSNLFNLLIIGLLDLMWKR
                     GPILNSLGSAPAVVGVLSIIVISIGGLGILIHGNLNLATSWPVSPVSIVLIVVFLAAL
                     FAIFKEEQNSDSYEEHNIYASASLKKASLVYLTSALIVVVAAYFLAHTGDSLADEMGW
                     AKSFMGTQFLALSTSLPELAASIAAIRIMAPELAISNLLGSNLFNMGFVLFL"
BASE COUNT          677 a          422 c          583 g          696 t
ORIGIN      
        1 ataccggtgg tgtagacaat atattccctc accatgaagg ggaaattgcc caaagcgaag
       61 ggtataccgg cttccaggta gtcaatcatt gggtccatgg gcaacattta ctggctgatg
      121 gagtgaagat ggccaaatca gctggtaatg ctttcataat acctgatctc gtggagagag
      181 gaatcgatcc gctggcattt cgttatcttt gtatgacggc taagtatcgg acgaggataa
      241 atttcacttt cacttcatta aaagcatgcg aaacagcact taataaattg cgtaggcact
      301 atattttatt cagcaaaaat tctcaaaatc agactttgaa caatgaaacc gtcaaagaat
      361 ggaaaaaaag gctaattgga attgtatgtg aagatctgaa tatgccaggg ctggtagatg
      421 ggatatggag actaatagag tcatctgatg ctgctgattc cgataaagca gccatcttaa
      481 gctctattga taacttgatg ggacttggtt tggattctac ggatgatatg tacgatttac
      541 ctgagaaaat aatcggtaaa tcggatgaaa ggacggagct gcgagcatct cttgattatg
      601 aacgcgctga tcggttaagg acagagatgg aagagctggg gtatatggtg gaggatgcta
      661 aagattattc cactataagg cttaaagatt cttcagaaat tagggaggaa agatggaaga
      721 caatatcttc ctcgtctgaa atagcctcca aagtttttag tgaatctgtc gtacccgtca
      781 cagtgggagt ggttgtcaat ggctatccgg atgacacaca acgttgtgtg tccagcttga
      841 ttaaatggac ggaatctgac cagacggaaa ttattgttat agacaatggg tccactgatt
      901 taacagggga actgttagag gaaatgacga aggatcaaga aaaccttcac attttgcatg
      961 ctgatcatac cttgggggac ggatctgcaa agaatatttt attgaagatg gcccagggag
     1021 aaatttttgt gcttcttgat ccaagtgtag aaattaaggg aaatttcata aagcctatac
     1081 aaggtttatt gaaagatcca gaagtcggta tcgcgggtct agctggactt aaaacagagg
     1141 atttgatgca ttttcatgac ggcgaagggg aatccggaca agtggatgca atgcagggct
     1201 attgtttcgc attaagaagg gaggatctta aaaaagttgg gttaatgaga gagtctttta
     1261 gatattacag gaacctagat ttggactata gtttccaatt taaagaacag ggactatcca
     1321 taatagccga ccacactttg ccaatggaat tgcatgagca cagaggctgg actgccttgt
     1381 cggagggtga gcgggacgag ttaagtaaga aaaactatgg taggttcctt aaaaaatgga
     1441 gaggtcggac ggacctattg atttctaaca aataaatacc tatacagttc acatagtatg
     1501 ctctagtatt ttcacgtttt aatttctttg ccttagtgct aattattttc cggagggagc
     1561 tttcattttg gatagtttgt cattgcctct attatgggtg gttctcgctg gcctcgcagc
     1621 aataattctg atatcggcga cgcagctgac aaagaatgca gatatcatag cttttaagac
     1681 tggtttaggt agatcttttg ttggtgttgt tctattagca accgcgactt cactaccgga
     1741 attggctact ggcgtgagtt ctgtgttgtc tataggaggt gctggaggag ctgaccttgc
     1801 cgcaggggac gcattcggaa gtaatctctt taacctcttg atcataggcc tacttgacct
     1861 tatgtggaaa aggggaccaa tattaaactc cttggggagt gcccccgccg ttgtaggtgt
     1921 tttgtcaatt attgttatat caatcggtgg gttaggaata ttaatccacg gaaacttaaa
     1981 cctggccaca tcttggcctg ttagtccagt ttcaattgtt ctgatagtgg tctttttggc
     2041 ggctcttttc gcaatattta aggaagaaca gaattcagat tcctatgaag agcacaatat
     2101 ttacgcttcc gccagcctaa aaaaagctag tcttgtgtac cttacatcgg ctcttatcgt
     2161 cgtggtggct gcctacttct tagcccatac aggcgacagt cttgctgatg agatgggttg
     2221 ggcaaaaagt tttatgggta ctcaattcct tgcgttaagt acctccttac ctgaactggc
     2281 agcttccatt gccgcaatac gtattatggc tccggaattg gctataagta atttgctcgg
     2341 aagtaatcta ttcaatatgg gatttgtatt attcctgg
//
LOCUS       DASCNS010000118         3869 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001007489, whole genome shotgun sequence.
ACCESSION   DASCNS010000118 DASCNS010000000
VERSION     DASCNS010000118.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001007489"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            72..719
                     /locus_tag="QF838_04200"
     CDS             72..719
                     /locus_tag="QF838_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012900.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HJO59924.1"
                     /translation="MTLFKICGLKDSRNALVARENGASFLGFVFVHGVRRELSLDLAI
                     QILNEYRNESGRGGPALVGLFANQPIEEVNEIISQCGLDYAQLCGDEDKSYWDEVDAE
                     VIKQVKIDFGVNSKLINSDLDNISGAGYIPLLDKYEKGALGGTGKTFDWNVIEQFNLS
                     GKFILAGGLDSENVSQAIRVSRPWAVDVSTGVETDGAKDESKIFAFAAAVNDVKS"
     gene            788..1180
                     /locus_tag="QF838_04205"
     CDS             788..1180
                     /locus_tag="QF838_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJO59925.1"
                     /translation="MSYSFNHVHLKAPDPGATADWYVKAFEFEIINDENPRPQGDRFI
                     RCRTKDGIAVNISSDRTGEAMGVGDAAAHWGLEHFGIEVDDIDAEFKRLEDLGAKILD
                     GPTKTPTGLIIGFIQAPDDVRIEVMQLP"
     gene            1200..1583
                     /locus_tag="QF838_04210"
     CDS             1200..1583
                     /locus_tag="QF838_04210"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HJO59926.1"
                     /translation="MPRETFSPNPANKPSGFSPITKSGNLLFVSGQVPVNSKGDLIGE
                     DDCYLQAKQCFLNIESALDSVGATNDDIVKITAFIVRPEDYSKYATQRLELFPEDGPA
                     SSTVFISGLVSPKFLIEIEAIAVLG"
     gene            1590..1931
                     /locus_tag="QF838_04215"
     CDS             1590..1931
                     /locus_tag="QF838_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HJO59927.1"
                     /translation="MDDCLFCKIYGGEISSEILYKDDYCFVIKDINPVAPVHLLIIPS
                     VHLAYLQSFEVEDFKILNSMYKVAIELAITEGVSESGYRLVINQKRDSGQQVSHLHLH
                     LIGGHNLAGIG"
     gene            1937..2521
                     /gene="yqeK"
                     /locus_tag="QF838_04220"
     CDS             1937..2521
                     /gene="yqeK"
                     /locus_tag="QF838_04220"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HJO59928.1"
                     /translation="MTRQIDKLYDRVAEMVASLPLPLKEHINRTCDLARRLGKIHGVD
                     DKRCALGALAHDLARHLEGSQLLMQSREKNIPLNSYELDSPVLLHGPLAAVWLESDLG
                     CDDEEVVNSVRYHTTGRPSMTNVEKIVFIADKVEPEKLTGNSGLILVAEKIEVDLDES
                     ILEYLKLRIRSVMQQNKVVHPLALETWNYLIQEE"
     gene            complement(2529..3035)
                     /locus_tag="QF838_04225"
     CDS             complement(2529..3035)
                     /locus_tag="QF838_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HJO59929.1"
                     /translation="MPIKIAVISDTHCYEWHDIHPDMRTLVAECDIAIHCGDIVRQPV
                     VEGFLKSAKRGLIVHGNSDPVDLRESLPYTQVIEVEKIKIGITHPAWGGPEFPPSELF
                     KDFDEALDIILFGHTHEPLIQNISGTLFVNPGQGYKSFMVDGTMAVITIDNGVADAEI
                     VVIESGRS"
     gene            complement(3059..>3869)
                     /gene="coaBC"
                     /locus_tag="QF838_04230"
     CDS             complement(3059..>3869)
                     /gene="coaBC"
                     /locus_tag="QF838_04230"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HJO59930.1"
                     /translation="MFDNAATQANVTKLVTRGAFIAGPANGRLASGLIGQGRMVEPSD
                     IISSANMILGKDGDLSGKKIVVSAGGTEEAIDPVRNITNRSSGKMGYAIAEAARDRGA
                     IVSLVAAPNALPKPVGILVTKIQSTEEMKKVIDTECDGADALIMAAAVADWRPLEKLD
                     SKAKKSTSDTWSLKLVKNPDIIASIKNDSLIKVGFAAESEDLIENSKVKIKSKNLDFI
                     VANDITAKDSGFSVDTNKVTIIHSNGNIESLPLMSKYDVAHSILDRVKSGF"
BASE COUNT         1106 a          749 c          857 g         1157 t
ORIGIN      
        1 gtgggcgcgg ttttggtggg agaatctttg gtaacagcag atgatcctgc aattgcactt
       61 aggaatttac tatgactttg tttaaaatat gtgggttaaa ggactcacga aatgcccttg
      121 ttgcccgaga aaatggggcc tcttttctgg ggtttgtatt tgtgcacggt gttaggcgtg
      181 aattgagttt agatttggca atccaaattt taaatgaata cagaaatgaa tcaggtcgtg
      241 gagggccagc tttggttggc ttgtttgcga accaaccgat agaagaagta aacgaaatca
      301 taagtcaatg tgggttagat tatgcacaac tatgtggcga tgaggataaa tcttattggg
      361 atgaagttga tgctgaggtg atcaaacaag ttaaaatcga ttttggtgtc aatagtaaat
      421 taatcaattc ggatttggac aacatatctg gcgcgggata catacctttg ctagataagt
      481 atgagaaggg ggctttgggg ggcacgggta aaacttttga ttggaatgtg atcgaacaat
      541 tcaacttgtc aggtaaattt atattagctg gagggctgga ctctgaaaac gtatcccaag
      601 cgataagagt ttcacgacca tgggccgtag atgtatcgac gggtgtcgaa actgacggtg
      661 ctaaagacga aagtaagata ttcgcctttg ctgcagctgt aaatgatgtt aagtcatgaa
      721 tgagaatgtt gacagagatt aacacctaat ttaacctgct attttaatat aatcggagga
      781 attcaaaatg tcgtattctt ttaaccatgt tcaccttaaa gccccagacc ctggtgcaac
      841 tgcagattgg tatgtgaaag cttttgagtt tgaaatcatc aatgatgaaa acccccgtcc
      901 ccagggagac agattcatta ggtgccgcac taaggatggt atagctgtaa atatatctag
      961 tgatcgaacc ggggaagcaa tgggagtggg agacgccgca gctcattggg gattggagca
     1021 cttcggaatt gaagtggatg acatcgatgc agagtttaaa agattggaag atttaggtgc
     1081 aaaaatatta gatggaccaa ccaagacacc aaccggtcta attatcggat ttatacaagc
     1141 accggatgat gtacgcatag aagtgatgca gctaccttaa tggaactagg tcaggaacaa
     1201 tgccaagaga aactttttcc cccaatccag ctaataagcc atctgggttt tctccaatca
     1261 ccaaatccgg caatctgctt tttgtgtcag gtcaggtacc tgtaaattct aaaggagatc
     1321 ttatcgggga agatgattgc tatctccaag ccaaacaatg cttcttgaat attgagagtg
     1381 ctttggactc ggtaggggct actaatgatg atatcgttaa gataacagca ttcattgtta
     1441 ggcctgaaga ttattcaaaa tatgcaactc agagactaga actgtttccg gaagacggtc
     1501 cagctagcag cactgtattt attagcggat tagttagtcc aaaattcctt attgaaatcg
     1561 aggcgattgc agttctggga taggtaaaca tggacgactg tcttttttgt aaaatctacg
     1621 ggggcgaaat atcttccgaa attttatata aagatgacta ttgttttgtt attaaagata
     1681 ttaaccctgt tgcacctgtg catttgctga tcattcccag tgttcatttg gcataccttc
     1741 aatcttttga agtcgaagat tttaagatat taaactctat gtacaaggtc gctattgaac
     1801 tggctattac ggagggagta tctgaaagtg gttatagact cgtgatcaac cagaaaagag
     1861 attctgggca acaggtttca catttgcatt tgcacttgat tggcggacac aatctagcag
     1921 gtattggttg ataaatgtga ctcgtcaaat tgacaaactt tatgatcggg tagcagagat
     1981 ggtcgcttcc ctcccgttac ccttaaagga acatataaat cggacttgtg atttggccag
     2041 aaggctaggc aaaattcatg gggttgatga caaaagatgc gcactaggcg cacttgccca
     2101 tgatctagca aggcatcttg aaggatccca acttttaatg caatctagag aaaaaaatat
     2161 ccctttaaat tcttatgaac ttgatagtcc ggtgcttcta catggacctt tggcagccgt
     2221 ttggttggag tccgacctag gatgtgacga tgaagaagtg gtaaattcag ttaggtatca
     2281 cacaactggg cggccatcca tgacgaatgt cgaaaaaata gtttttattg cagacaaggt
     2341 agaaccagaa aaattgacag gtaattcagg actgatattg gtcgcagaaa aaattgaagt
     2401 tgatcttgat gagagtattt tagaatattt gaaattgagg atcaggtctg taatgcaaca
     2461 gaacaaggtt gttcatccac ttgcacttga aacctggaac tacctaatac aagaagagtg
     2521 aaatgttttt acgaacgtcc tgattctatt actacaattt ctgcatccgc cacaccgttg
     2581 tcaatcgtaa taacagccat agttccgtcc accataaatg atttatatcc ttggcccgga
     2641 tttacaaaca aggtaccgct aatattttgt attagaggtt catgagtgtg cccaaacaga
     2701 attatatcta aagcctcatc aaaatcctta aatagctccg aaggcggaaa ttcaggtcct
     2761 ccccaagctg ggtgagtgat acctatcttt attttctcaa cttctatgac ttgggtgtac
     2821 ggaaggcttt cccgcaaatc cacgggatca gagtttccat gcactattaa ccccctcttt
     2881 gctgatttca aaaatccttc cacaactggt tgtcggacta tatctccgca atgaatcgct
     2941 atgtcacatt cagccactaa agtgcgcata tctgggtgaa tatcgtgcca ttcataacaa
     3001 tgcgtgtctg aaatgaccgc tattttaata ggcatagttt gtctttttgt caatttggtt
     3061 aaaagcctga cttcaccctg tctaatatgg aatgggctac atcatactta gacattaatg
     3121 gaagagattc aatgttccca tttgagtgga tgatggttac tttatttgtg tctactgaga
     3181 acccgctgtc ctttgcagta atgtcatttg cgacaataaa gtctaggttt ttagatttga
     3241 tttttacttt gctgttttct atgagatcct cactctccgc tgcaaacccg actttaataa
     3301 ggctgtcatt tttaatactg gcaataatgt cagggttttt taccaatttt aatgaccaag
     3361 tatcagaagt actttttttg gctttggaat ccaatttttc caaaggtctc caatcagcga
     3421 cggccgctgc cattatcagg gcgtcggctc catcgcattc ggtatcgatc acttttttca
     3481 tttcctctgt gctttgaatt ttggtcacca aaatacccac aggttttggt aaagcattag
     3541 gagctgcaac taggctaacg atagcgcccc gatctctcgc tgcctctgca atcgcgtagc
     3601 ccatttttcc tgatgaacgg ttggtgatat ttcttactgg gtctatcgct tcttcagttc
     3661 ctccagcact cacgacaatt ttcttacctg acaagtcccc atccttacct aatatcatat
     3721 tggccgaact aattatgtca gagggttcaa ccatccttcc ttgtccgata aggcctgagg
     3781 caagcctccc attggctggg cctgcaataa aagctcccct ggttacaagt ttggtcacat
     3841 ttgcttgggt agctgcgtta tcaaacatg
//
LOCUS       DASCNS010000119         9733 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001017027, whole genome shotgun sequence.
ACCESSION   DASCNS010000119 DASCNS010000000
VERSION     DASCNS010000119.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001017027"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="QF838_04235"
     CDS             <1..1132
                     /locus_tag="QF838_04235"
                     /inference="COORDINATES: protein motif:HMM:NF017727.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HJO59931.1"
                     /translation="FLEVQKIIMYLIVPRIRNTSMNFPWPQRMTLGLVLLAIICRFFG
                     QTVNSFNVNEILLGMSGICLLIAAGIFIFTIGKTLSKGKSRHGLPEIWFWASLFWCFI
                     ASLLNLVIVLQMIDRGARIVSFTMEDSFVHVTLIGFVANFVFGISLRVLPGLLFLPTP
                     RFSLNKVSLILINVGISVIAIQPIIVVSNWWLLIATLIELAGFISYVLSVHIYNRRVT
                     VRQYVLNTYGRYEWFLRSGYFWLLVGGVLQVWLSVGHLNHNIAVSIELAAPVVHVWGL
                     GFITMIIVGMASRMVPMFEGAVLPLQRIMDLVFILLNLGVILRLGFGIIPSHNSWTGL
                     ALSGSLSTISITLFALIICLTLNPSSRNRYIEIAIEFGKNRQ"
     gene            1571..2713
                     /gene="sfsA"
                     /locus_tag="QF838_04240"
     CDS             1571..2713
                     /gene="sfsA"
                     /locus_tag="QF838_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HJO59932.1"
                     /translation="MLRPKLVSETPLTAKFLSRINRFVIKCHLGGVGEVKAYMANPGR
                     LGELLLPGAPLLIETVSDDSARKLRYAASAVFTGESFIGLNTHQANAVARYLLKNKLI
                     PTLENANIEKEEISIGRNRFDFLMSENRQKFLMEVKSVTLSANKIAMFPDAITERGTR
                     HLNELAKSKESIIKPVVLFISQGLGNNLFMPDYHTDLVFSRTLLALKERLRIVPIEIK
                     WDQNLELSDSVKLLDVPWSFLKTRMEDKGAYILVMEIPKACNIAIGSIGRVDVNPGYY
                     LYAGSGMNGLSSRISRHLRKKKKLHWHVDFLRQVAGKVKAYPIRTPQYIEEELVLALE
                     QVFSPILPGFGASDSNLGTHLFYSPSDPFLTKSFQVLLSKFRFNDS"
     gene            complement(2700..4163)
                     /locus_tag="QF838_04245"
     CDS             complement(2700..4163)
                     /locus_tag="QF838_04245"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJO59933.1"
                     /translation="MVEEFDVLIVGAGISGVGGAFHLKTQRPNTKFIVIEALDSFGGT
                     WWSHRYPGIRSDSDLHTFGYRFKPWTGPPIATADEILNYMSEVITENELGNHIRYNRK
                     IVSANWSSLEKRWEVTAKLTEGGEEEHFKIKFLWMCQGYYRHQEGYMPKWPDVEKYQG
                     ELVHSEAWPEELDYEDKEVIVIGSGATAATIIPAMASDCKHITMLQRSPTYFRPGRNQ
                     IELAETLRELNIDEEWIHEIVRRKILYDQEIFSKRTFEEPEKVKEELIEGVKSLLPAE
                     FDVETHFTPKYRPWRQRLAYVPDGDLFQGISEGKASVITDEIEAFTKEGILLKSQQEV
                     KADIIVSATGFNMNVMGDIDFQIDDKSVDFSDTVTYRGMMFTGIPNMLWIFGYFRASW
                     TLRVDLLSDFVCRLLNHMEDIGASCVEVALRDEDKNMELLTWMDSYNFNPGYLLRATS
                     ILPKRGDKAEWQHTQDYWREKDEIPAINLNASEFNYR"
     gene            complement(5070..5501)
                     /locus_tag="QF838_04250"
     CDS             complement(5070..5501)
                     /locus_tag="QF838_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59934.1"
                     /translation="MIEYTKLRKSGEATMALVAAASGTPGVFWTMNRYEDQSEADKDL
                     EAIFDSEAGKKRDEIQKKLAEKLIYFRTEIVVPMTNVTSEKPGAFNAMHLLPAKGRSR
                     DFMPRARKVVDYMADKGHTVGFISARSGNINRCWIILAARY"
     gene            5979..6932
                     /locus_tag="QF838_04255"
     CDS             5979..6932
                     /locus_tag="QF838_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HJO59935.1"
                     /translation="MTRKSLYAIGALILLASVACESRTFKAEVEAARTAGIPDPPPGA
                     VALRLSFEYESNPQLGLGIRVHEPTSEIDTIWATESLPKGIDMPVGPAIVDRTVFLKP
                     GESRMVALAYKNLVGQDVSILFAPHQDAPSNLASHVDFKCLCFPTFNQVPYKVPAEGS
                     WYKVIQVGVGSDMPAGSRVDSLYKVLFESLRATANLTTPSTTGGRSTLGMVRLTEVEL
                     GRSLAARNGCASCHSTGGGPGVGSTNYVGTPWDGLFGREVVLENGSKVTADEAYLRES
                     IIDPNAKIVKGFSAGIMPQGFGDRLSPEDIQALIEYIKAIR"
     gene            7136..7576
                     /locus_tag="QF838_04260"
     CDS             7136..7576
                     /locus_tag="QF838_04260"
                     /inference="COORDINATES: protein motif:HMM:NF021622.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59936.1"
                     /translation="MNMNDLYEKFITQILIENTDNFRVENRKDIYLDETNRYKMNPDI
                     LLRFKNSDRVALVADYKYKKLRHSGSSNPDMHQVLSYCTSTMSNHEVLIYSLHEVENT
                     PPAPILNTETAIRQMTIDLGGPMENLVNECSRLAFQEKEVQRLG"
     gene            complement(7590..7676)
                     /locus_tag="QF838_04265"
     tRNA            complement(7590..7676)
                     /locus_tag="QF838_04265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7641..7643),aa:Leu,seq:taa)
     gene            8026..8232
                     /locus_tag="QF838_04270"
     CDS             8026..8232
                     /locus_tag="QF838_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HJO59937.1"
                     /translation="MSEKNFVATLLLAILLGSLGAHRFYVGKTGTGIVMLLTLGGLGI
                     WKLIDIIIIAVSNFKDSNGLPIKP"
     gene            8411..8656
                     /locus_tag="QF838_04275"
     CDS             8411..8656
                     /locus_tag="QF838_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59938.1"
                     /translation="MSRGSFGTVGVHHFYVGRWLHGLFDLSLLVAAGIFFFASAWILA
                     GLLIFVDVVHNTYFVYKLIIGEYRDGYGKLIRVPGPS"
     gene            complement(8777..>9733)
                     /locus_tag="QF838_04280"
     CDS             complement(8777..>9733)
                     /locus_tag="QF838_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HJO59939.1"
                     /translation="KKLAEQSETGPATIMIGGLGLGMISTVIPGLVIIVAMWLSYEFA
                     ELYGIALAAVGMLSTLGITLATDAYGPVADNAGGIAEQAGLDPEVRERTDALDSLWNT
                     TAATGKGFAIGSAVLTALALMAAYAQVVGLETLDVMNVKVMMGLIIGAIMPFLFAALT
                     MQAVGRAAYAMVQEVRRQFREIKGLLEGTADADYARAVDISTQGAMKEMVLPGLIAVV
                     VPVVVGIILGPGALGGLLIGSIATGFLFAIMMANAGGAWDNAKKYIEAGNLGGKGSDQ
                     HSAAVVGDTVGDPFKDTSGPALNILLKLMSIVALVFGPLFIA"
BASE COUNT         2617 a         2230 c         2061 g         2825 t
ORIGIN      
        1 atttttggag gtgcaaaaaa tcatcatgta tttaattgtt ccacgtatac gtaatacttc
       61 aatgaatttc ccctggcctc agagaatgac tttagggttg gtgttattag cgataatttg
      121 ccgctttttc ggccagaccg ttaattcttt caatgttaat gaaatcttat taggcatgtc
      181 ggggatttgc cttttgattg ctgccggaat attcatattt acaattggta aaacgctatc
      241 gaaaggaaaa tctcgccatg gattgccaga aatttggttt tgggccagcc ttttttggtg
      301 ttttatagcg agtttgctta atcttgtgat tgttttgcaa atgatagatc ggggcgcgag
      361 aatcgtttcg tttacaatgg aagattcttt tgtgcatgtc accttaattg gatttgttgc
      421 caatttcgtt tttggaatta gcctccgagt attacctggt ttattattct tgcctacacc
      481 aaggttttct ctaaacaagg ttagcctcat tctcatcaac gtgggaattt cggtgatagc
      541 aattcaacca atcatagtcg tctccaattg gtggttgcta attgcaactc tgatagagtt
      601 agccggattt atttcatacg tcttgtctgt gcatatatac aaccgcagag tgactgttag
      661 gcaatatgtg ttaaatactt atggaaggta cgaatggttc ttgcgatctg gttatttctg
      721 gttactcgta ggaggagtcc tgcaagtgtg gttatcagtt ggccatctaa atcataatat
      781 agccgtctct atagaattgg ccgcacctgt tgtgcatgta tggggattgg ggtttatcac
      841 aatgatcatt gtaggcatgg cctcaagaat ggtaccaatg tttgaagggg cggtactacc
      901 tctacaaaga atcatggatt tagtttttat acttttgaac ctaggggtaa tactaaggtt
      961 gggtttcgga attatcccat cccataattc gtggactgga cttgccctat caggatcttt
     1021 gagcacaatt tcaataaccc tttttgcttt gattatttgc ttaacactca acccttcgtc
     1081 taggaatagg tatattgaaa ttgccattga atttggaaaa aatcgccagt agtgcttgtt
     1141 ctagaactct cccaggtaag ggttatcagt cacagcattc cacaacgatg gctatctaca
     1201 gccatgtaga ccagtaaatg caaagagaat cctcaaagaa ggtgaatgca attctggatg
     1261 gtttcttttt acccttcgta aatggcttta taaagtgcaa gggaactttt cccacccatg
     1321 ttctagtact tgaacgtcct tttcttaaag tgctaacctt actatcactc ttcctatttg
     1381 gttgacgcat ttgaaaaata gttgttaata ctattaatta actgttttta aaaacgtcag
     1441 tcttgaagca attatctaag atttttgaga cctttgaggt gtctctcaga cattatgtct
     1501 tgttctgaat ttcccctcct tatcttcgaa gcacgaaggg attcgagcca aatcttatga
     1561 agctaggata atgttacgtc caaaactcgt ttcagaaacg ccattaacag ccaaatttct
     1621 ttcccgaatt aacaggtttg taatcaaatg tcacttaggg ggggtcggtg aagttaaagc
     1681 atatatggct aaccccggta ggttaggcga attgctgttg cccggcgctc ctcttcttat
     1741 cgaaacggta agtgatgatt ctgctagaaa actgcgttac gccgctagtg cagtttttac
     1801 cggcgagtca ttcataggcc tcaacaccca ccaggccaat gccgtggcaa gatatttgtt
     1861 aaaaaacaaa ctcattccga cccttgagaa tgcaaatatt gaaaaggagg aaattagtat
     1921 tggcagaaat cggtttgatt ttcttatgtc ggaaaaccgg caaaaatttc tcatggaagt
     1981 taagtctgtc actttgagtg ccaacaaaat cgcaatgttt ccagatgcaa taacagagag
     2041 gggaactcgc catttgaacg aattagcaaa gtcaaaagaa tcaataatta agccggtcgt
     2101 tttatttatt tcacaagggt tggggaataa tttattcatg ccggattatc atacagatct
     2161 ggttttttcg cggaccttac tagccttgaa agagcggctc agaattgttc ctattgaaat
     2221 taagtgggac caaaatttag aactaagcga ctccgtaaag cttcttgatg tgccctggtc
     2281 ttttctaaaa acgaggatgg aagataaagg ggcctatatc ctggttatgg aaattcccaa
     2341 agcctgtaat attgcaatcg gttcaattgg gagagtggac gtaaaccctg gatattatct
     2401 ttatgcagga tctggaatga atggtttgtc ctctaggata tccagacacc ttcgcaaaaa
     2461 aaagaagctg cattggcatg tagatttcct tcgccaggtg gcaggtaagg tgaaagccta
     2521 ccccattaga actccccaat atatcgaaga ggagttggtt ctggctctag agcaggtgtt
     2581 ctcccctatt ttacccggat ttggagcatc tgactcgaat ctaggaaccc atctgtttta
     2641 tagtccgtct gacccattct taactaaatc atttcaagta ctacttagta aatttcgttt
     2701 taacgatagt taaattccga agcattcagg tttatggcag gtatttcatc cttttccctc
     2761 cagtaatctt gagtgtgttg ccattctgct ttgtctccac gttttggcaa aattgatgtt
     2821 gcacgtaaca gatagcctgg gttgaaatta taggaatcca tccatgtcaa aagctccatg
     2881 tttttatctt cgtcacgaag tgcgacttcc acacaagacg caccgatatc ttccatatga
     2941 ttgagtaatc tgcatacaaa atccgaaagg agatctaccc gaagagtcca actggctcta
     3001 aagtacccga aaatccacag catattaggg attccagtaa acatcatccc tctataggta
     3061 accgtatctg aaaaatccac tgatttatca tcgatctgga agtcaatatc acccataaca
     3121 ttcatattga aaccagtcgc acttactatg atgtctgctt tgacttcttg ttgagatttc
     3181 agcaagatac cttcttttgt aaaagcctca atttcatcgg taataactga agccttgccc
     3241 tccgaaattc cctgaaaaag atctccgtcg ggcacgtagg ctaaacgctg gcgccatggt
     3301 cgatatttgg gggtgaaatg cgtctccaca tcaaactcgg cgggtaaaag agatttaacg
     3361 ccttctatta attcttcttt cactttttca ggttcttcaa atgttcgctt ggagaagatc
     3421 tcttgatcat agagtatttt ccgtcgaact atttcatgga tccattcttc atcgatattt
     3481 aactctctta aggtctcagc caattcaatt tggtttcttc caggtctgaa ataagtggga
     3541 gaccgctgca acattgtaat gtgcttgcag tcagatgcca tggctggaat tatggtggca
     3601 gctgtggccc cggagccaat tacgataacc tctttatctt cataatcaag ttcttctggc
     3661 caggcttcgg aatgaactaa ctccccttga tatttttcca cgtcaggcca tttcggcatg
     3721 tatccttcct gatgccgata atagccttga cacatccaaa gaaatttaat tttaaaatgt
     3781 tcttcttctc caccctctgt taatttagca gttacttccc aacgtttttc taagctggac
     3841 cagttggctg acacaatttt gcgattgtac ctaatatgat tgcctaactc attttcagtg
     3901 atcacttcag acatgtagtt taagatttcg tcagctgtgg caataggagg acctgtccac
     3961 ggcttaaatc ggtagccgaa agtgtgtaaa tcactgtccg atcttattcc aggatatcga
     4021 tgagaccacc acgttccacc aaaactatcc agggcttcta taactatgaa cttggtattt
     4081 gggcgttggg tctttaggtg aaaagcgccg ccaacacctg aaattccggc cccgactata
     4141 aggacatcaa attcttctac catatacggt atttttcccc catgaatgaa aaaaacggcc
     4201 actctgtgtg agaaagaagc ataacttatt ggttcttcac gcgctcgggc aaaaattcga
     4261 ttctatatga gtaaaccatg aagatccaaa actaacccat tcatctgtgg cttccacggt
     4321 ctaatttcag tctattccgt ggtctctttt gtttcggttg gccagcagct gggcgtgatt
     4381 ttctaaccct tctaaccttc tggtatacct ctggatggtt ccttttcacc cttcgtaaat
     4441 gggtgtataa cgttttccac cgacatcacc gtcaatggtg tcaaactgtg actgagaccc
     4501 cgacagtgga gggatattat cagcgtcatc taccgtcgac ccaggcatgc atttagaagc
     4561 ttcagagaag atgaatgaag tcttagaagg ctagtatttt aaaacaggag ccacaaaggc
     4621 tagggtaggg gtgcgaaaaa gtggtgccca ggaagggact cgaaccccca ctctcttacc
     4681 gagaagcgga ttttaagttg tacacacaga tttcgtgatc taccagttcg taccttttta
     4741 tgactagaaa cacgccatta caggagtcca tgtcgctcat tttctgcctg atagcagccc
     4801 gtgttgggaa tttggtcggc aaaatatcgg cagaattgaa ccggttgggg tagggcggca
     4861 cctttatatt ggtatctaat ggctcagact atttctcata gccactcaat gcccatccaa
     4921 agttgtaaat caaattagtc tctagtcgat agtaataatc tgcccctctg gtattctgga
     4981 gttgcctcca aactttcaac catatcaaac caggcattaa cgaatgccgg atcttcataa
     5041 ttggtcttgg aatttaagtc agcttctcct taatatcttg cggcaagtat tatccaacag
     5101 cgattaatgt tgccactcct tgcggaaata aacccaacgg tatggccttt gtcggccatg
     5161 tagtcaacga cctttcttgc tctaggcata aaatccctag accttccttt agctggtaga
     5221 aggtgcatag cattaaaagc tcctggtttc tcacttgtca cattggtcat cggcacgaca
     5281 atttctgttc tgaaataaat caatttttcc gcgagttttt tctgtatttc gtctcgcttt
     5341 ttaccggctt ccgaatcaaa tattgcctct aaatctttat ctgcttctga ctggtcttca
     5401 tacctgttca tggtccagaa cacaccagga gtaccgcttg cagcggctac taatgccatg
     5461 gtcgcctctc cggattttct tagtttcgtg tactcaatca tgtcttgagt gaattctctt
     5521 cttgagtgaa ttctctgcca attcctaaag ccgaattcca cctagttatg tttattacag
     5581 ccattattga ccccctagat aacatatctt taaataatag ctagagataa atattgcggt
     5641 atagtatcac tatagtgatc tttttattaa cacacggtgt gactggaaat cgtactgttt
     5701 ggggtagata aatggggtac gcacgaaggg gtgtcaggta gcattctctc caatgcgaac
     5761 cgtggacagg gtagggcttc tatattaggt taggtacagt ctccgaccaa ttgtttaata
     5821 ttccctaaac attgatgaaa gaagttggtg atatgaaacc aggactcctt acattttcga
     5881 aaagttgcgg gatcatcatc gccgttattc tcgtgatgtc ccatagccat ccagttttta
     5941 cggctttctt gggttgagaa gcggctagag gaacgaacat gactcgtaaa tctctttacg
     6001 ccataggggc cctgatcttg ctggcatcgg tcgcctgtga gtcgcgcacg tttaaggcgg
     6061 aagtagaagc agcccgcaca gcgggaatac ccgaccctcc accaggagcg gtggcgctaa
     6121 ggctcagctt cgagtacgag tccaacccgc aattggggct cggcatccgg gtccatgagc
     6181 ctacatccga gatcgatacc atctgggcca cagaatcgct tcccaaggga atcgacatgc
     6241 ccgtgggccc tgcgatcgtc gacaggaccg tcttcctgaa gcctggcgag agtcgcatgg
     6301 tcgccctggc atacaagaac ctcgtcggtc aggacgtgag tatcctgttc gcgccgcatc
     6361 aggatgcccc cagcaatctg gcctctcacg tcgatttcaa atgcctctgc ttccccacgt
     6421 tcaatcaggt accttacaaa gtccccgcgg aagggtcctg gtacaaggtg atacaggtcg
     6481 gtgtggggag tgacatgccc gcaggttcga gggtagactc cctgtacaaa gtcctcttcg
     6541 agtcactcag ggccacggcc aatctcacca ccccctccac gaccggcggt aggtccacat
     6601 taggtatggt ccggctcacg gaggtggagc ttggacggtc actcgccgcc cgtaatggct
     6661 gcgcatcgtg tcactccacc ggcggcggcc cgggggtcgg ctcaaccaac tacgtgggca
     6721 ctccctggga tggactcttc gggcgggagg tagtgctcga gaatggctcg aaggtgacgg
     6781 ccgacgaagc ctatcttcgc gagtccatca tcgaccccaa cgccaagatc gtgaagggat
     6841 tctcggctgg aatcatgccg cagggattcg gcgaccgact ctccccagag gacattcagg
     6901 cattaatcga gtacatcaaa gccatcaggt aggtaatttt cgcgtccgaa ccaaccggtg
     6961 cagcaggtcc caccgtcgaa gccgaaatcc tggcatgacc cccgatagag tgctaatacg
     7021 gaatgacacc agtgatttca ccggtagaaa gtccgctctc ccccttggaa ctcccaaggc
     7081 ccagcaccac actcttctcc accgagcgcc tctatagtat tcgcctgcat ttttcataaa
     7141 catgaacgat ttatatgaaa aattcatcac ccagatactg attgaaaata ctgataactt
     7201 tagagttgaa aaccgaaaag acatatattt ggatgaaacc aatagatata aaatgaatcc
     7261 cgacatcctg ctgcgattca agaattctga ccgggttgct ttggtcgcag attataaata
     7321 caaaaagctc agacactcgg gttccagtaa ccctgatatg catcaagttt tgtcttattg
     7381 cacttcaact atgtcaaacc atgaggtttt aatttattca ctgcacgaag ttgaaaatac
     7441 tccccctgca ccaattttaa acaccgaaac cgccatcaga caaatgacca tagacttagg
     7501 tggcccaatg gaaaatttgg ttaatgaatg tagccggcta gcatttcaag aaaaggaggt
     7561 acaaagacta gggtaggggt gcaaaaaagt ggtgcccagg aagggactcg aacccccact
     7621 ctcttaccga gaagcggatt ttaagtccgc cgcgtctacc attccgccac ctgggcattt
     7681 tacattccaa ctgctaaaag cagagcccac attataactg gttaagagta agaaatgtgc
     7741 cctggttaga tttgatcaat aatgatcaaa tctgtctggc aaaaggaact aaattcggcg
     7801 ttgtatagaa ccgttagtga ccatttcaac caaaagttct ccaaaattat gcctgggcat
     7861 ccggactgaa aatcccccac ccgcaatgta atccaacagc catcatagca tcaggttata
     7921 ccttcatata ttcagctata tgggttacaa attgcttcga aaataggcac gggcatacta
     7981 tagttaaggt agaaatttaa cagttagtgc agagaggttt ttggcatgag tgagaaaaat
     8041 ttcgtcgcta ccctgcttct agctattttg ttaggtagtc ttggtgcaca ccgattctac
     8101 gtcggtaaaa caggcacagg aatagtgatg ctgctgacgc tcggcggttt aggaatatgg
     8161 aagttaatcg acattattat tattgcagtc agtaacttta aggacagcaa cggattacct
     8221 ataaaacctt agacaaaaaa aagatttcat ttatttgaat ttcacggatt tttcatatgg
     8281 tttgcctgaa ttgcagatct agcaatgaaa tagggactta attttgcagc tcctgtggta
     8341 gcaaatttgg tacccaggaa tctaccgggg atttttcaga ttcctctcgc aaaccctatg
     8401 tgacagctgt atgtctcgcg gcagttttgg aaccgttggt gtccatcatt tttatgtcgg
     8461 aaggtggctc catgggttat ttgacttatc tcttttggtt gccgctggga tattcttttt
     8521 tgcctctgcc tggattctag cgggactgct tatctttgta gatgtagtac ataacaccta
     8581 tttcgtctac aaattgataa taggggaata ccgagacggt tacgggaagc taatcagggt
     8641 cccgggccca tcataaaagc catctgaaag ttcttctgcc tatcttaaaa taaccataaa
     8701 gctaccttaa ataaaaatga gggcgatgat acatcgccct cattcaaatt cacaacatag
     8761 tatatatttc tgattcttat gctataaaca aaggtccaaa cactaatgcc actatggaca
     8821 ttaatttgag tagtatgttc agtgctggtc ccgatgtgtc cttaaatggg tctccaaccg
     8881 tatcacctac aaccgcggct gagtgctgat ctgatccttt tcctcccaaa ttacctgcct
     8941 cgatatactt cttggcattg tcccaagcac cgcctgcatt tgccatcatt attgcaaata
     9001 agaacccggt ggcaatggac cctataagaa gcccacctag ggctccaggg cccaaaatta
     9061 tgccaaccac aactggaacc acaacggcta taaggcctgg gagaaccatt tctttcattg
     9121 cgccttgcgt agatatatct acagcccttg cgtaatctgc atcagctgtt ccttctagca
     9181 ggccttttat ttctctaaat tgtctcctaa cctcttgcac catagcatat gcagctcttc
     9241 ctacagcctg catagtgagc gccgcaaaca agaagggcat aatagcacca attatgagac
     9301 ccatcataac ttttacattc attacatcca gggtctcaag gcctactacc tgagcataag
     9361 cagccattag ggctagtgct gtaagaacag cggatcctat agcaaatcct tttcctgtag
     9421 ctgcagttgt attccacagc gaatctaaag catcagttcg ctctctaact tctggatcaa
     9481 gtccggcttg ctcagcgatc ccgccagcat tatcagccac tgggccataa gcatcggttg
     9541 caagagttat acctaaagtt gacaacatac cgactgcagc aagggcaatt ccatacaatt
     9601 ctgcgaattc ataagaaagc cacatcgcta ctatgataac tagaccgggg attactgtgc
     9661 taatcatccc aaggccaagt ccaccgatca taattgttgc agggccagtc tccgactgtt
     9721 cagctaattt ttt
//
LOCUS       DASCNS010000120         4080 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001020195, whole genome shotgun sequence.
ACCESSION   DASCNS010000120 DASCNS010000000
VERSION     DASCNS010000120.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001020195"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2569
                     /locus_tag="QF838_04285"
     CDS             <1..2569
                     /locus_tag="QF838_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HJO59940.1"
                     /translation="MFDIDRGSINSSNASNLASNITYFGNRLDSELNRFVIRTELDKS
                     LQRYDTRILFTKLQMYVVLIMITFVVLYYVVALSSLVVEERRFEVSKLRSRGASSSQI
                     LSVFVIEGATISFFATLLGPLIAVGGISLLGFLPGFHEINDGTFLSARLTYPALFMSI
                     LGGVLSFLALMIPSIQASRLMVSDERTKSNRPNRLTIISRYYIDVIVLIVSLFLFNQL
                     NEQGSMAARGLLGDVKVNQIMLAVPAIMLIASALVILRIFPLVMSIASRLLSRYLPVG
                     VALIMWQISRNPSNYSRLVLLLILMTGLGIFVSSFGGTLNKNFEDRVYYSHGSDVRLS
                     SVSLNSSGLSKPLTKKIESMDGVSAVSASARMMSTDVTKTFGSDSIAVLGIDTNKFEQ
                     SVWYRDDFSESSLSEISNTLQETDTKGIELPDKSRSFGVLVKSDTNRPTTALVARMKD
                     KNGRYFSFDLGRLDSGGWTLKQVEIFGRGRGRFQLFPTRPLTLMSIGIVETNPQKKLT
                     SGSVLMDSVRVRLATGEVVNLEDFRDINGWQTIDSSTSSANDRLGISEISARSDASAI
                     FTWSEGPPMTMRGIYPSTQFRPISAIVNSDFLINTQYSLGDQLKLSIGGHRIDVVLRD
                     KVRYFPTLNPIEDDFIVVGLDPLIHKLNIGSLFGSTDPNELWIDYEDGITNETKTGLK
                     EDLINDSPFPYGKLWDTESMLEINRVDPLVKAGWHAILVIAFGSILLLSAIGFFSHAY
                     ISYRNRRFQFALLRTLGFSTRQLITTIWVEQVLIIVLGMVVGTWMGGKLSAAVMPFLS
                     SDDYGNQVIPPFVIQVDWVNLSITYAVMVLIFATVIGSLIFLARRLVLNKMLRLGDT"
     gene            complement(2566..3492)
                     /gene="fmt"
                     /locus_tag="QF838_04290"
     CDS             complement(2566..3492)
                     /gene="fmt"
                     /locus_tag="QF838_04290"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HJO59941.1"
                     /translation="MKIVFMGTPYFTIPVLDKISSNGHDIVAVYTQPDRTSGRGRKVT
                     MSAVKEYAISKNLRVLQPQNFRKDLSQIHILDSFKADIAVVAAYGILLPSEILDLLPH
                     GCINLHPSLLPKYRGASPVSTAILESEKVTGTTIMQLDSGIDSGPILAQQEAPIGEEE
                     LCNELTEKLFYLGADLIVETISKLAKGIIVPRQQDHENATYTKKLVRQDGLVDWKDGA
                     DVILRKIRAHHPWPGSYTYFNGKNLKIIEARISENYKLGPGCVSVHDNAVYIGTSDDS
                     LIVSQLQSEGAKITSATDFARGHRDFENSILG"
     gene            3627..>4080
                     /locus_tag="QF838_04295"
     CDS             3627..>4080
                     /locus_tag="QF838_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59942.1"
                     /translation="MKLNLVIANFLANHPYMILIVTLFLTLLLISPLFFLAPKEQASP
                     NPPDEVYELQEEIDDKFPTPFHFASFVLQAKKGDVISREVLSELKLNRDRLVALDVDG
                     GLSVTEEGKPRGTLEKQSYLASYYDFELATQINGIISVLETIEITLANQ"
BASE COUNT         1093 a          745 c          892 g         1350 t
ORIGIN      
        1 gatgtttgac attgacaggg gaagtatcaa ttcctcaaat gcttcgaatt tggcgtcgaa
       61 tataacctat tttgggaacc gattagactc agaattaaat aggtttgtca ttcgcactga
      121 gctagataag tctttgcaaa gatacgacac acgtattttg ttcactaaac tgcaaatgta
      181 tgtcgtcttg ataatgatca cctttgtggt gctctattat gtggtggcac tctcttcact
      241 ggtagttgaa gagagacgtt ttgaagtttc caaacttaga agtagaggtg ctagttcgtc
      301 acaaatattg agtgtatttg tgatcgaagg agctaccatt tcattttttg ccaccttgct
      361 tggtccattg attgccgtag gcggaataag tctgttggga tttttaccgg gattccatga
      421 aatcaatgat ggcacttttc tttctgccag gttaacttat ccagccttat tcatgagtat
      481 attaggtggc gttttaagtt ttcttgcttt aatgattcct tccattcaag catcaagatt
      541 gatggtgtct gatgaaagaa ctaaatcgaa taggcctaat cggctcacga tcatatctag
      601 gtactacatc gatgtcatag tcctaatagt ttctctattt ttatttaatc aattgaatga
      661 gcaaggctct atggcagcac ggggcttatt gggggatgtt aaggtcaatc aaattatgtt
      721 agctgtccca gccattatgt tgattgcctc tgctttagtt atattgagga tattcccact
      781 tgttatgagt attgcaagcc ggctgttgtc taggtatcta ccggttggtg ttgctttgat
      841 catgtggcaa atttcacgaa atccttctaa ttattccaga ctagttttac ttttaatttt
      901 gatgacaggg ctaggtattt ttgtctccag tttcggtggg accctcaaca aaaattttga
      961 agaccgtgtt tactattccc acggttctga tgtgaggcta agttcagttt cacttaattc
     1021 cagcgggtta agtaagcccc tcacaaaaaa aatagagtcc atggatggtg tgtctgccgt
     1081 tagcgcgtca gctcggatga tgagtactga tgtaacaaaa acatttgggt ctgattctat
     1141 agctgtcctt ggcatcgata ctaataaatt tgaacaaagt gtgtggtatc gggacgattt
     1201 ctcagaatct tcactatcag aaatttctaa tacactgcaa gagacggata ccaaaggaat
     1261 tgaattgcct gataagtcac gatcatttgg agtattggtt aaatctgata caaatagacc
     1321 aactacggcg ttggtagcta gaatgaaaga caaaaatggc agatattttt catttgattt
     1381 aggtaggctg gacagtggtg gatggacact gaagcaagtt gaaatctttg gtagaggaag
     1441 aggaagattt caactgtttc ctacgcgacc tctgacattg atgtcgatag gtatagttga
     1501 aactaatcct caaaaaaaat taacttccgg ttctgttttg atggatagtg tgagggtaag
     1561 gttggcaact ggtgaagtgg ttaatctgga ggatttcaga gatattaatg gttggcaaac
     1621 aatagactcc tccacctcct ctgcaaacga taggttagga atttcagaaa taagtgcgag
     1681 atcagatgct tcagccattt ttacatggtc tgaaggtcct ccaatgacga tgagagggat
     1741 ctatccttct acacaattta gacctatttc tgccattgtt aattctgact ttttaattaa
     1801 cactcaatat tctttgggtg atcagctaaa gttgtcgatt gggggccacc gaattgacgt
     1861 ggtattgcgc gataaagtca ggtactttcc tactctcaat cctattgagg acgattttat
     1921 agtagttggg ctggacccac tgattcataa gcttaatatt gggtctttgt ttgggagtac
     1981 tgatccaaac gagctttgga tcgattatga ggatgggatt acaaatgaaa ccaagacggg
     2041 cctcaaagag gatttgatta atgattcccc tttcccttac ggcaaattat gggatacaga
     2101 atcaatgctt gagatcaatc gcgtcgatcc tttagtcaaa gccggatggc atgcaatatt
     2161 ggtaatcgca tttggttcga ttttgctttt aagcgcgatc ggattttttt ctcacgccta
     2221 catatcatat agaaaccggc ggttccagtt tgcgttactt agaacccttg ggttttctac
     2281 tcgtcagctc ataactacta tttgggtgga acaggtttta ataatagtgc tagggatggt
     2341 cgttggaacc tggatgggag gaaaattatc agcggcggtt atgccgtttt taagtagtga
     2401 tgattatggt aatcaggtaa ttccaccgtt tgtgatacaa gtagattggg ttaatctatc
     2461 gataacgtat gctgtgatgg tcctaatttt tgctaccgtc ataggatccc tgattttcct
     2521 ggcacggaga ctcgtattga ataaaatgtt gaggcttgga gatacttaac ccagaatgga
     2581 attttcaaaa tccctgtggc ctcttgcgaa atcagttgcc gaagtaattt ttgccccttc
     2641 gctttgtagt tgcgatacga ttaaggaatc atcggaagtg ccaatataca ctgcgttatc
     2701 atgaactgag acacatccgg gtcccaattt gtaattttct gaaatccttg cttctatgat
     2761 ctttaaattt ttcccattga aataggtgta tgaaccgggc caagggtgat gtgccctgat
     2821 ttttctcaat attacgtcag cgccgtcttt ccaatctacc agaccatctt ggcggactag
     2881 ttttttcgtg taggtggcat tttcatgatc ctgttgtcgg ggcactatta ttccttttgc
     2941 taattttgaa attgtttcca ctattaaatc ggcacctaaa taaaataatt tctctgttaa
     3001 ttcgttacac agttcctctt caccaattgg agcctcttgt tgggctagta tagggcctga
     3061 atctattccg gaatctagtt gcataattgt ggttccagta actttttccg attcaagaat
     3121 agctgttgat acgggcgaag cccctcggta ttttggtaat agggatgggt ggaggtttat
     3181 acaaccatgc ggaagcagat ccaatatttc agagggcagc aatattccat aggcggctac
     3241 tacagcaatg tctgctttaa aactatctag tatatgaatt tgggaaaggt ccttccggaa
     3301 attttgcggc tgtagcaccc gcaaattttt agatatggca tattccttta ccgcactcat
     3361 agtgactttt cttcctcgac ctgatgttct gtctggttga gtgtagacag ctactatgtc
     3421 atggccattg gatgaaattt tgtccagaac agggatggtg aaatatggtg ttcccataaa
     3481 tactattttc ataaataaag ttaactcctg ctgtcagcct tctgaattat aagaccctct
     3541 atccttttac ggatatttaa tgagactatt aagggatagc ttttgttgat ggttcttttt
     3601 ccagtttgct aaggaatgcc agttaattga aattaaatct cgttatagcc aactttcttg
     3661 cgaatcatcc atatatgatt ctaatagtaa ccttgttttt aaccttgctt ttgatttccc
     3721 ctttgttttt cttggccccg aaagaacaag cctccccgaa tcctccggat gaagtttatg
     3781 aacttcagga ggaaattgat gataaatttc ctactccatt ccattttgcg agttttgtgt
     3841 tgcaggcaaa aaaaggtgat gttatttctc gggaagtgct ttcggagttg aagttaaata
     3901 gggatcgttt agttgcatta gatgttgatg gtggtttatc agtcacagaa gaaggtaaac
     3961 caaggggaac tttggaaaaa caatcatacc ttgctagcta ctatgacttt gagctagcaa
     4021 cgcaaatcaa tgggatcatt tctgttttgg aaaccatcga gattaccctt gcgaatcaag
//
LOCUS       DASCNS010000121         1327 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001027884, whole genome shotgun sequence.
ACCESSION   DASCNS010000121 DASCNS010000000
VERSION     DASCNS010000121.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001027884"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="QF838_04300"
     CDS             complement(<1..463)
                     /locus_tag="QF838_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HJO59943.1"
                     /translation="MTHNTKDYGAGTLSDQIRSRVCRGVNGLDIHYLEAGFENKNNPL
                     LLLLHGFPELSYSWRKMILPLSKSGYHVVAPDQRGFGATTGWNNSYVSDLSSYYQNNL
                     VRDMLGLVSALGYEKVKSVIGHDSGAGVAGWSALIRPDIYESVVMMSAPFTG"
     gene            complement(496..>1327)
                     /locus_tag="QF838_04305"
     CDS             complement(496..>1327)
                     /locus_tag="QF838_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012913.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HJO59944.1"
                     /translation="DMISVGSPAIGPKEWLQLSNRINTVLNSTNVDGVVVTHGTATLE
                     ETAYFLHLTVKSKKPVVITGAMRPPTAFGTDADLNLIDAIRVAASPHATDKGVVTVLN
                     NEIHCARDVYKANTLRVETFRANELGFLGYSDSDGEVLFYRSPLRKHTYSTDFDVKTL
                     TELPRVDIVYAYAGSDNLFIDAVRQNKSDGMVLTGFGSGTLPPIMLETGSETTQEGIP
                     VVLASRSTAGRVVITPRITKLGFLVADNLHPQKARILLMLGLTITKDPDILQEMFMVY
                     "
BASE COUNT          355 a          291 c          293 g          388 t
ORIGIN      
        1 cgcctgtgaa aggagcactc atcataacaa cagattcgta tatatcaggc cttatcagag
       61 ctgaccaacc cgcaacaccg gcaccagaat cgtgacctat aacacttttg actttttcat
      121 aacctagtgc agaaaccaaa cccaacatgt ctcgtacaag attgttttgg taataggaag
      181 aaagatccga aacgtatgaa ttgttccatc cagtagttgc gccaaatccc ctttgatctg
      241 gtgctactac gtggtagcca gattttgata gaggtagaat catttttctc cagctataag
      301 ataattccgg aaatccatgg agcaatagta ggaggggatt gtttttgttt tcaaaacctg
      361 cctcgagata atgaatatca agaccattga cgcctcggca gacccgtgat cttatctgat
      421 cagacagggt accggcccca taatcttttg tattatgtgt catatctatt cctgttttgg
      481 ttgaagttat gactactaat aaaccataaa catttcttgc aaaatatcgg gatccttggt
      541 tattgttaaa cctaacatca ataatattct ggctttttgt ggatgaaggt tatcggcgac
      601 cagaaaccct aatttggtga tgcgtggtgt aatgacgact ctgcctgcgg tagaccttga
      661 tgccaaaact acaggaatcc cttcttgggt ggtttcagat ccagtttcca gcataattgg
      721 aggcaaagtt ccagacccaa aacctgttaa taccataccg tcggatttat tttggcggac
      781 tgcgtcaata aacaggttgt cggagcccgc gtaggcataa actatatcta cccgcgggag
      841 ttctgtgagc gttttaacgt caaaatcagt tgagtaagta tgttttcgga gaggacttct
      901 atagaagagt acctctccat ccgaatcaga gtaacccaag aatcctagtt cgttggcacg
      961 gaatgtttca acacgtagag tattcgcttt gtagacgtct ctagcgcaat ggatttcatt
     1021 gttaagtacg gttacgacgc ctttatctgt tgcatgaggg ctcgcagcaa ccctgatagc
     1081 gtctatgagg tttagatctg catctgtgcc aaatgcagtt ggtggccgca ttgccccggt
     1141 aataacgacg ggttttttgg attttactgt caaatgcaaa aaataggctg tctcctctag
     1201 tgtggcagtc ccgtgtgtta ctacaacccc atcaacattt gtagagttta aaacggtgtt
     1261 gatccggtta cttagctgga gccattcctt ggggcctatt gccgggctac ctacgctgat
     1321 catgtcc
//
LOCUS       DASCNS010000122         2786 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001033151, whole genome shotgun sequence.
ACCESSION   DASCNS010000122 DASCNS010000000
VERSION     DASCNS010000122.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001033151"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            56..835
                     /locus_tag="QF838_04310"
     CDS             56..835
                     /locus_tag="QF838_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HJO59945.1"
                     /translation="MEEGHVITAATIGVLASMGMSHVRAIRKPVVSILSTGNELLSPG
                     EKPAEGKIFDSNSSSIMASVIQLGAIPRYIGIARDEMGSVREKLREAMESDLVISSAG
                     VSKGDYDMVKDVMSEHGDLQFWSVRMRPAKPLAFGTLNRGSKTSVPLIGLPGNPVSSL
                     VAFEQFCRPAILKMMGKTNLHRPTIQAVLKDSIVNYDSRRVYARVIVEQTEDGYTAKT
                     TGHQDSNILTSMVHANGLAICPEEEESKDPGDIVTVIMLDD"
     gene            904..1830
                     /locus_tag="QF838_04315"
     CDS             904..1830
                     /locus_tag="QF838_04315"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HJO59946.1"
                     /translation="MSQSLAYFRGQIVPIEQANVNVMTHALHYGTAVFEGIRGNWSDL
                     EQKMFVYRMREHYIRLLQGCRMLMMDIGKTADELCDITENLIKECGYQQDLYIRPLAY
                     KSEELVANLKLQDLSSDFTLIMVPFGAYLDSDGALNCCTSSWRRVDDTIIPPRVKISG
                     HYVNSILAKTEATLAGFDEAIMLTPDGFVSEGSGENLFMINDGKLVIPPVSDNNLTGI
                     TRDSAITIAREDLGMEVLERRIRRSELYFADEVFLTGTAAHIQAVGRLDSRNIADGKT
                     GEYTTAINEIYQKAIRGENPKYRGWCTEVSVN"
     gene            1986..2162
                     /locus_tag="QF838_04320"
     CDS             1986..2162
                     /locus_tag="QF838_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59947.1"
                     /translation="MFAAPIIFFTTADRIVNDYQGGLDANEQAVLFALSAITALIIRK
                     LSSTAERVIRKNIK"
BASE COUNT          820 a          516 c          625 g          825 t
ORIGIN      
        1 ccctggaact aacgtatgag taaaaggcaa ggacataaag gaagcagatt cagtgatgga
       61 agagggtcat gtaatcactg cggctaccat cggagttctt gcgtctatgg gtatgtctca
      121 cgttcgagcc attagaaaac ctgtggtatc aatactttca accggaaatg aattgctcag
      181 ccctggggaa aaacctgctg aaggcaagat atttgatagt aattcaagct ccattatggc
      241 ttctgtaatt cagctcggtg ccataccaag gtacattggg atagctcgag atgagatggg
      301 atcagtgcgt gagaagctta gggaagcgat ggaaagcgat ttagtcatct cgtcggccgg
      361 tgtttccaaa ggtgattatg acatggtcaa agatgtaatg tcagagcacg gagatttgca
      421 gttctggtca gttcgaatga gaccggccaa accgctggct tttggtacat tgaatagagg
      481 ttcgaagacc tctgtgcctt tgatcgggtt acctggtaat ccagtaagtt ccctggtagc
      541 ttttgagcaa ttttgcagac cggcaatctt gaaaatgatg ggcaaaacta acctgcaccg
      601 acccactatt caggctgtat tgaaagattc tatagtgaac tacgattcac gccgggttta
      661 tgcaagagtc atcgttgaac agaccgaaga tgggtatacc gctaaaacta ccggtcatca
      721 agattcaaat attttaactt ccatggttca tgccaacggt ctagcaatat gccccgagga
      781 ggaagaaagc aaagacccag gtgatatagt aacggtcatt atgctcgatg attgatatag
      841 ctgtaattga gattaattgc agctataata acggacaatt ttgtgaatat agaggagact
      901 taaatgtcac aatcccttgc ctacttccga ggacaaatag ttcctattga acaagcaaat
      961 gtgaatgtca tgacacatgc cttgcattat gggacggccg tctttgaagg gatcagaggt
     1021 aattggagcg acctggaaca gaaaatgttt gtctatcgaa tgcgagaaca ttacataaga
     1081 ttgttgcaag gctgcagaat gttgatgatg gacattggta aaacagcaga tgaactctgc
     1141 gacatcactg aaaatttaat aaaagagtgc ggttaccaac aagatttata catcagacct
     1201 ttagcctata aaagtgaaga acttgttgcc aatctaaagc tacaggacct cagtagtgat
     1261 ttcactttga taatggtgcc atttggcgcc tacctagact ctgacggagc tttaaattgt
     1321 tgcacttcct cctggcgcag agtggatgac acaattatcc ctcctcgtgt caaaatttca
     1381 gggcactacg ttaatagcat attggcaaag actgaagcta ctttggcagg gtttgatgag
     1441 gcgattatgc taaccccgga cggattcgtc tcagaaggtt ccggtgagaa tttatttatg
     1501 ataaacgatg ggaaattagt gataccacca gtatctgata ataatttgac aggcataaca
     1561 agagattctg cgattactat cgccagagaa gacctaggaa tggaggttct agagagaagg
     1621 attaggagaa gcgagcttta ttttgctgat gaagtgtttt tgactggtac agctgcacat
     1681 attcaggcag taggtagact agatagtagg aacatagcgg acggaaaaac tggagaatat
     1741 accacggcta taaacgaaat atatcaaaag gcaatacgag gtgaaaaccc taagtaccgt
     1801 gggtggtgta cagaagtatc tgtgaactag cctccaacta cagttttgat aaatgtgcaa
     1861 tgtttggatt ccacttagat tattttcttt gctgcgtact agccgtattc ggtttattag
     1921 tcactgttgt gccctatcgt agttcttcat tatcacttgc accatattgt cttggaattt
     1981 gtttaatgtt tgctgcaccg attatctttt ttaccacggc tgacagaata gtcaacgact
     2041 atcaaggtgg gttagatgca aatgaacaag ctgttctctt tgctctatcg gcgataacag
     2101 ctttaataat tcgtaagctt tcttcaacgg cggaaagggt tatacgaaaa aatatcaaat
     2161 aattctgatg caagacttca ttaacactat catttggttt gaccaaaaaa tgttcctagc
     2221 tattaattca atggtaggaa cttcttcggt aattgattct tcggcaagga ttatagcaag
     2281 tgactatttg gtgccagttt ttctgttttt gctgttaatt ttcctttggt ataaagaaag
     2341 ctccataggt aaaaaaatca aaacccgact ctctgtgcta aacagtattt cgaccctttt
     2401 gttggcttgt ctaatagtat ttgctctaaa tcaaattttg tttcgagaaa ggccatttaa
     2461 tctgatggag gcaaatttga ttttttatac cccaacagac tcttcctttc catcaaactc
     2521 tgtggcagct gtaatgggtc tcacgattcc cgttttcaat gtttatagaa agatggggtg
     2581 cctgatgatg tcctctagtt tttgcttggc attctgcaga atatatgtgg gcatccactt
     2641 tcccggcgac gtacttggtg gaatattaac agcactagtt gcctttttat tctcgacact
     2701 tttgattagc ctgtttccga aacaaataca ttttgtatta ttgttatttg aaaaaggaaa
     2761 atggctataa ttatttaggc tataac
//
LOCUS       DASCNS010000123         6377 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001036067, whole genome shotgun sequence.
ACCESSION   DASCNS010000123 DASCNS010000000
VERSION     DASCNS010000123.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001036067"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="QF838_04325"
     CDS             complement(<1..1279)
                     /locus_tag="QF838_04325"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HJO59948.1"
                     /translation="MSTMPITQSDLQASLPDTTSGIQTNRIRDEISITRDIHGIPHVV
                     GSNIYDAFFGQGFATAQDRLWHMEFDRRKAFGRWAELVGEQGLDSDITMRSFQIEDSV
                     INDLIHLNEETMLMLTAYSDGVNAFIDTTSSLPIEYKLTNSKPEPWSPKDCLAVYKVR
                     HIMMGVFEGKYWRSQLLNNLGPERTDDLIKGYQQGHLLIVPPLDRYSGPELNGIESLQ
                     SSIESIAWMQEDPEAGSNSWAVHGSKTNSGKPLLAGDPHRGLDTPNCYYQNQVTCPDF
                     DVIGLSFPGCPGFPHFGHNAKVAWCVTHAMADYQDLYLEKITPKPNGSTYTWKDQELD
                     LECKKESIKIAGLPDHQISLKSTRHGPIISENSLGTRAISFKYTSTAGINRGFEALIP
                     QMTASSISEADQAMEKWVDPCNNYMFVDVEGGIQ"
     gene            1402..1483
                     /locus_tag="QF838_04330"
     tRNA            1402..1483
                     /locus_tag="QF838_04330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1436..1438,aa:Leu,seq:tag)
     gene            1554..1817
                     /gene="ffs"
                     /locus_tag="QF838_04335"
     ncRNA           1554..1817
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="QF838_04335"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1866..2459
                     /gene="rsmA"
                     /locus_tag="QF838_04340"
     CDS             1866..2459
                     /gene="rsmA"
                     /locus_tag="QF838_04340"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HJO59949.1"
                     /translation="MSHSINPAKKSLGQHFLHDGKVLTKIVKTAKVTETDHILEIGPG
                     KGHLTKKLLLQGCSVTAIELDHDLIIGPLKDIAVNPKLEIVEGDARKVNLKKLIANTP
                     YFKLVANLPYNAGTNILRSILLSDLPPQLSVVMLQKEVALNITAFPGKSNILSNLFQV
                     YYDTNFHFNVQPTSFTPQPKVMSGVISLAKKKYTLTA"
     gene            2702..3565
                     /gene="ispE"
                     /locus_tag="QF838_04345"
     CDS             2702..3565
                     /gene="ispE"
                     /locus_tag="QF838_04345"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HJO59950.1"
                     /translation="MEIKAPAKVNLSLEILGKRLDGFHDISSITQTISLFDKLQIERD
                     DQLTLESPGFELHSNKNIVYSAAKKMREQYSVNLGARMVLQKKIPVSAGLGGGSSDAA
                     AAIKILNKIWKLNLPLQELISFASTIGSDVPFFLEGGTSFVQGRGELIRELPDCKIGW
                     LVIVVPNVRLEKKTKSMYSNLTENNYTSGGLSRKLEARIRNGGDCPPQLMFNGFLEVA
                     KESIPAVEKAFSIFNTVGVHEIHLTGSGPSLFTIVKRKEIATALSLLLTHKYQLESYV
                     VGNVSRDTFQV"
     gene            complement(3562..3765)
                     /locus_tag="QF838_04350"
     CDS             complement(3562..3765)
                     /locus_tag="QF838_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59951.1"
                     /translation="MPKTAQVGFQTIIEDKIILEGARVLNISYIGPLALLYITKIAKH
                     PKAIDIEANSRPDAAPAISMSTN"
     gene            4061..4759
                     /locus_tag="QF838_04355"
     CDS             4061..4759
                     /locus_tag="QF838_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024660.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HJO59952.1"
                     /translation="MFNFDLHVHTVKGSTDSGLLPEQLIAESKRIELNGVFLSEHGGG
                     WTPTQIETEFGETDLTVATGLEVNTDMGHIIVIGLDSYVSGIHKLKVLREKIDEVGGI
                     LIAAHPLRNFFNRPPYNVNLLFGGWKVPPETPEQAAEHELFSLVDCIEVTNGANSEKE
                     NAFTLEIANYLNIPATGGSDSHSIQGIGRSFTIFENDIPDRETLIAELKAKRFYPAEG
                     LNIGKVQKFENTSS"
     gene            4881..6272
                     /gene="mgtE"
                     /locus_tag="QF838_04360"
     CDS             4881..6272
                     /gene="mgtE"
                     /locus_tag="QF838_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HJO59953.1"
                     /translation="MLNSIIGSLAKPLGTTEDIDQKVYSLSSHMESRNRGTALFSFLS
                     LPSADQADVILRLSREHQLNLISWVKPADFALIVEELETSEFIHLMTDIPPSEVSSIL
                     ELTSPPIAADILKNLSQSDSYLLTQQMNHADEILALMSYDAKNAGGLMTTSFLSVNQS
                     MTAEAATEYIRSFSYTDLDDVSSVLVVDDQEFLVGIFNITRLVTAPLKHPVSSIIFPS
                     IISVSVDTDREECARIMERYNLLTLPVTDEYGRLVGTLKIEDMIFVLQEEATEDMYRM
                     VGVDEEEKLLSPIWLSVKGRFPWLFINLITVGFAAVVITLFQSTLSQVLALAVFIPVI
                     AGQGGIAGTQTLTLVVRSMALGEIRKSDSARLLGKELGIGLIHGLLLGLVAGIVAYYW
                     HGSEYIGAVVGVAMLGNLVVASISGVSLPLVLKALNIDPALASAVAVTTLTDVIGLVL
                     YLGLATLLLPVIA"
BASE COUNT         1829 a         1326 c         1306 g         1916 t
ORIGIN      
        1 attggatacc accttccaca tcgacaaaca tgtagttatt acatgggtcc acccattttt
       61 ccatagcttg atcagcttca gatattgagg aagcagtcat ttgtgggata agagcctcga
      121 accctcgatt gataccagcg gtggaagtgt acttaaaaga gatagccctc gtacccaatg
      181 aattttcaga tataatcggc ccatgcctag tagattttag tgagatttga tgatcaggaa
      241 gccctgcaat ctttatggat tcctttttgc actctaaatc taactcttga tctttccagg
      301 tatatgtact gccattgggc ttaggtgtta tcttttctag atatagatct tgataatctg
      361 ccattgcatg ggtaacacac caagcaacct ttgcgttgtg tccgaaatgt ggaaaacctg
      421 ggcaaccagg aaacgatagg ccgataacat caaagtctgg acacgtgacc tgattctggt
      481 aataacaatt tggggtatca aggcctctgt gggggtcgcc tgcaagcaga ggttttccgg
      541 aattcgtttt acttccatgt actgcccaac tattacttcc tgcttcaggg tcttcttgca
      601 tccatgcaat cgattctatt gatgattgaa gactttcaat accatttaac tcaggtccac
      661 tatacctatc taaaggaggg acaattagca aatggccttg ctgataccct tttattaaat
      721 catctgtccg ctctgggcct agattgttta aaagctggct gcgccaatac tttccttcaa
      781 aaacccccat cataatatgt ctaactttat atacagccaa acaatccttt ggggaccaag
      841 gttcaggttt agaattcgtg agtttatatt caattggtag ggaagaggta gtatctataa
      901 aagcatttac cccatctgaa taggccgtta gcatcaacat agtttcttca tttaaatgta
      961 taagatcatt tattaccgaa tcttctatct gaaagcttcg catcgttata tctgaatcta
     1021 agccttgttc accgaccagt tcggcccatc gtccgaatgc cttacgtcga tcaaactcca
     1081 tgtgccatag tcgatcttga gcggtggcga acccttgacc gaaaaaggca tcgtagatgt
     1141 tcgaacctac tacatgggga attccatgta tatctctagt tatggaaatt tcatctctta
     1201 tacgatttgt ttgaatgcct gaagtcgtgt caggcaaaga agcctgcaga tcagactgag
     1261 tgattggcat tgtactcatt acctctccct gggttattgc taagaaatca cctaatactt
     1321 cgacaactgt agaaaatatc aatgaatttc acaagtttct ccctttaatt aatacgccta
     1381 ctgtataatt taaccccgat cggggaagtg tcggaattgg tagacgagct agatttagga
     1441 tctagtgccg agaggcgtgt gggttcgagt ccctccttcc ccaccggatc acccaaagtc
     1501 cttttaattt atccgttgac actgttattt tactgaccta gtatggatat gggtcgtgct
     1561 aggcggggag ttagcggtgc cctgtattcg caaaccgctc tagcgaggct gaattcctgt
     1621 cataggtgcg taagaaatta cctctggcaa tttgtatagg tgttgatagt cgggtcctac
     1681 gcggcgggag cctgtgaact ccgtcaggtt cggaagaaag cagcggtaaa cggatactct
     1741 cgggtgccgt agggaagcct gattagagcc ataccaattg tatatgttgt tgcttcacat
     1801 caacgaacgg tgcacggccc gcttacgaaa atattgtatt ttccttttaa ccacatttgg
     1861 atagtatgtc ccattcaatt aatccagcca aaaaatccct tggtcaacac tttctccacg
     1921 acggtaaggt attaactaaa attgtaaaaa cggccaaagt gactgaaacg gatcacattt
     1981 tggaaattgg accgggaaaa ggacatctga caaaaaagct tttactgcaa ggatgttccg
     2041 taaccgcaat tgaattggac catgatttaa taataggacc tttaaaagac atagccgtaa
     2101 atcctaaatt ggaaattgtc gaaggtgacg cccgcaaagt aaatctaaaa aaactcatag
     2161 ccaatacacc atattttaaa ttggttgcca atctgcccta caatgcaggt acgaatatcc
     2221 tgagaagtat tttactatct gacctcccac ctcaattaag tgttgtgatg ttgcagaagg
     2281 aagtggctct gaatatcaca gcattccctg gtaaatccaa tattttatct aacctttttc
     2341 aggtctacta tgacactaat tttcacttca atgttcagcc gacatctttc actccccaac
     2401 cgaaggttat gtctggagta atatcattgg cgaaaaaaaa atataccctt actgcctaaa
     2461 tgcctccacc ttgattattt ttcatttttg acctgtggct ttgccgcacc tcgaaagcag
     2521 ctacacaact ccttatcgca aggattgata ataactatcg cagaagtgaa atatctaagc
     2581 tcccagttag gcattaacag cgaacttaga cccgcggcgt taggggttga tgcatgggtt
     2641 gcattgttca aaaaattcag agagcaaaaa taactttata ggcttggaaa aagatatgaa
     2701 tatggaaatc aaagcgccag ccaaggtaaa tttaagtttg gaaattctag gtaaaaggct
     2761 agatggattt catgatatct cgtccataac ccaaacaata tctttattcg acaaattgca
     2821 aatcgagaga gatgatcaac ttaccttgga aagtcccggc tttgaattgc attccaataa
     2881 gaatattgtc tattcagccg ccaaaaagat gcgtgaacaa tattctgtca atttaggggc
     2941 cagaatggtt ttgcaaaaaa aaatccctgt atcagccggg ctaggtggtg gtagcagtga
     3001 tgctgctgcg gccattaaaa tcctgaataa aatatggaag ttaaatcttc cactacaaga
     3061 actcatttcc tttgcttcta caattggttc ggatgtacct ttctttttag aaggaggcac
     3121 ctctttcgtt caaggtagag gagagttaat tcgggagcta cccgattgta agattggttg
     3181 gttagtgatt gttgtaccta acgttagact agaaaagaaa acaaaatcca tgtactccaa
     3241 cctaaccgaa aataattata cctccggagg cctatcgcga aaacttgaag cccgcatacg
     3301 aaatggtgga gattgcccac ctcaattaat gtttaatggc tttctggaag tcgctaaaga
     3361 gagcattcct gctgtagaga aggcattcag catattcaat acagttgggg tacatgaaat
     3421 tcacctaacc ggtagtgggc cctcactatt tacaatagtc aaacgtaaag agatagcgac
     3481 agcactatct ttgttactaa cgcataaata tcaattggaa tcttacgtgg tcggcaatgt
     3541 ttcacgggac acatttcagg tttagtttgt ggacatactg atagcaggtg cagcatccgg
     3601 tctactattt gcctctatgt cgattgcttt tggatgcttt gctattttcg taatgtataa
     3661 aagcgctaat ggtcctatat acgaaatgtt caacactcta gctccttcta agataatttt
     3721 gtcttcaata atggtttgga atcccacctg tgcggttttg ggcatactgt ttgccctcct
     3781 cttctcatat ttatcagata taagaccagg acccggtctt ttaagcccga attttttcta
     3841 caccttcttc gtaatcggta tttccttcct ttttttggtt atttcctatc aattcctctc
     3901 gataattttc cgaccaatgc tgggtatagt gggcggattt ctgttaattt ttgggtggct
     3961 gataccttat ttggctatat gaatacattt attcctaatt tagacttgaa tcacctcaac
     4021 cctcaactta gaatgtatag gttggcacag aggtttatgt ttgtttaatt ttgatcttca
     4081 cgttcatact gtgaaaggaa gtaccgatag cggattactg ccggaacaac taatcgcaga
     4141 atctaaaaga attgagctta acggtgtttt cctatcggaa cacggtggag gatggacacc
     4201 aactcagatt gaaacagagt ttggtgaaac cgatctgaca gtggctaccg gcttagaagt
     4261 taacactgat atggggcata taatagttat cggcttggac tcctatgtta gcggcattca
     4321 taaacttaag gtcttgaggg aaaaaatcga tgaagtgggc gggattctga tagcagcgca
     4381 cccgttaaga aattttttta atcgcccccc ctataacgtc aacttacttt tcggtggatg
     4441 gaaagttccc ccagaaaccc ctgaacaagc agcagaacat gaattattct cactggtgga
     4501 ctgtattgaa gttaccaatg gagccaacag tgagaaggaa aatgcattca ccctcgagat
     4561 agcgaattac ctgaatatac ctgccactgg tggcagtgac tcacactcca ttcaaggtat
     4621 tgggagaagc ttcactatat ttgaaaatga tatcccagac cgagaaactc tcattgcgga
     4681 actcaaagca aaaaggtttt atcctgctga gggactgaat atcggaaaag tacaaaagtt
     4741 tgaaaatacc agttcttagc taacttacgg ctatgaatat cccccataat atgatcttca
     4801 aatatcttta aattccactt cttaagtctt aaccttttat ggcactcaat tttcactgag
     4861 atataaaggg ttatttttat atgctcaatt ccattatagg tagcctggca aaacccttag
     4921 gtactaccga agacatagac caaaaggttt attccctgtc ttcccatatg gaatcacgga
     4981 atagaggaac agcgttattt tcctttcttt cccttccctc agcagaccag gccgatgtga
     5041 tcttacgatt aagtcgagaa catcaactaa acttaatatc ttgggtcaaa ccggctgatt
     5101 tcgccttaat tgtagaagaa ttagaaactt ccgaatttat acatttgatg accgatatac
     5161 ctccgtcaga agtatcaagt atcttagaat tgacttcacc tccaatcgcg gcggatatcc
     5221 taaagaacct ttctcaatct gattcgtatc tgctaacaca acaaatgaac catgccgacg
     5281 agattttggc attgatgtct tatgatgcca aaaatgccgg tggtcttatg accacctctt
     5341 ttctcagcgt taaccaatct atgacggcag aggccgctac tgagtatata cgtagctttt
     5401 cctatactga tcttgacgac gtatccagtg ttcttgtagt tgatgatcaa gagtttttgg
     5461 ttggtatttt taacataaca agactggtta ccgcaccact aaagcaccct gtttcttcta
     5521 taatttttcc tagcataatt tctgtctctg tagatactga cagagaagaa tgtgcccgga
     5581 ttatggaacg ctacaattta cttacattgc cggtcaccga cgaatatggt cgtcttgtag
     5641 gaacactaaa aatagaagac atgattttcg ttttgcaaga agaggctacg gaagatatgt
     5701 atcggatggt tggagtagat gaagaggaaa aacttctgag ccccatatgg ctttcagtta
     5761 aaggtcgctt tccttggttg ttcatcaatt tgatcacggt cggatttgct gctgtggtaa
     5821 taactctatt ccagtcaacc ttgtcacaag ttctggcttt agcagttttt attcccgtta
     5881 tagcagggca aggtgggata gctggtactc agactttaac attagtagtg agatcgatgg
     5941 cgctcgggga gattaggaaa tcagattctg cccgcctctt aggtaaagaa ctaggtatag
     6001 gtttaatcca tggtctccta ctcggattgg ttgcaggcat cgtcgcttat tactggcacg
     6061 gcagtgaata tattggagcg gtggtagggg ttgctatgct aggaaattta gttgtggcaa
     6121 gtattagcgg agtatcactt cctttagttc taaaggcgtt gaatatagat cctgctctag
     6181 cctctgcagt ggctgtcacc acgttaacag atgtcatagg gctagtttta tatctcggct
     6241 tggcgacact tctactccca gttatagcct aaataatttt aaccattttc ctttttcaaa
     6301 taacaacaat gcaaaatgta tttgtttcga aaacaggtta atcaaaagtg tcgagaacat
     6361 aaaggcaact agtgctg
//
LOCUS       DASCNS010000124         4965 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001042670, whole genome shotgun sequence.
ACCESSION   DASCNS010000124 DASCNS010000000
VERSION     DASCNS010000124.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001042670"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="QF838_04365"
     CDS             <1..315
                     /locus_tag="QF838_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59954.1"
                     /translation="MEIRQIEAALTDFDKALHIEPNNSYGYCNRAVAYNYMRDFNKSI
                     SESTLAVEINPQLPEAYVVRGIAKVQMGALAAADLDFEVAESLGYKRIDIEERLIALR
                     KV"
     gene            complement(871..2010)
                     /locus_tag="QF838_04370"
     CDS             complement(871..2010)
                     /locus_tag="QF838_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HJO59955.1"
                     /translation="MPDNSFDNPGSLDEPILTAKEIEKYREYLSDYGKEIFFSEEETI
                     VDYGDITTSIDLLVSGEALVEVPVNEKWIPVAWIKSGSVIGEIAFLDSYPRTARVIAR
                     TSCRLYRVDRADFTQLSERDPEKALEFISRISQIMAYRLRRIEQFDAVEQGRDDMRKE
                     LAADLHDQTMSELSAILMHLGLLKLTLDGHAESAADIEHLVTMVKNADVNLRQLVREK
                     GHDDLAIVGLEESLRIFFEGLENEVVPFSIKIAYTSNQIDQGGLPGPVARDLYQIIRQ
                     SVLNSVEHANPEMIQVEIIWKQEGISFFVKDNGVGFNQSSIDNVPEAGHFGLLNLKLR
                     TERVGGSVNIISAEGKGTNVIGSIPITRRISEYIEPQTVQYTLSA"
     gene            complement(2032..2697)
                     /locus_tag="QF838_04375"
     CDS             complement(2032..2697)
                     /locus_tag="QF838_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HJO59956.1"
                     /translation="MANILVADDSPFIRTAYKRVLETQADFVVSSVCEDGLEAVNKCS
                     ELEIDVAILDIRMPRLDGISASKTIRENNPDVSVVVVSAYDDWSYVKDLIETDSKSKA
                     YVLKNSLDDIGELIRIVRKVMQGHLILDNALIEKLIGYYERHSSASENLSGRELEVIS
                     RGCKGMSLQEIASDLEIPPADVEVIQVLVEEKLNVSGDDYLSAQANSTIAFLNLCVSL
                     TLG"
     gene            complement(2715..3362)
                     /locus_tag="QF838_04380"
     CDS             complement(2715..3362)
                     /locus_tag="QF838_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59957.1"
                     /translation="MMDGMDSIRRTIYDKFGNVMKTLKADVEVQATSEINSQDISSSE
                     ETLTEDTSVDGAPVSDALWDLSELDPEIYEDVFEPTLMEATPPPLQNTITSSAAGNDT
                     PDDADSTIIEPHTLQKPRNFQTVSLDSIDDNEEQNSQAEEDPSREDSGEEEQPSVLAS
                     NIEVDSAEESEMGDLFAEKIEVRPQVRQLLDKYGTVSIDQLTKELKEVSELLSRK"
     gene            complement(3362..>4965)
                     /locus_tag="QF838_04385"
     CDS             complement(3362..>4965)
                     /locus_tag="QF838_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HJO59958.1"
                     /translation="EKVSSDNQVIGFDLFSQLTYMAILSRGGVTRDRLLEYCGRQRYL
                     TAVFFEYINLMARSMGVEYTRAFQAVAQRAKAPRVKSLLLRFSASISSGSSESDFVLQ
                     EARAEAEHYSNDYERNVENLRKWTDAYAAVLVAVTLIMVVSQVSSLLGSTSASFIYLM
                     LFTLGGVTSVGVYLIYKVAPFETITYDPVSGTTPDRKMAKFLFIICGPTGLALGVMGF
                     LVLLEMGVSSVTASSSIFGLTGISLLPSAFLAMRDASRVSNIDQELPVFLRAIGSIAG
                     SGGVTLTEALRRIDTNSYQYLKDPLERLRTRLESGLPSSECWEMFSRETGSELVSRTA
                     GMFVDGVEVGCNPGQIGEICSDYAQNISELRAKRKLTSSTFSFLTMTMHSVMALILVF
                     VLEIIISFNSKLGSIVGVSDRENCLNQVVVPPGVEAAGIKLPRPEELCASVDVFQMGN
                     IATLQTTIILAVAILTVANALAPKFSEGGNNLKIITFLAVTCLTSAVIMLAVPRVTAA
                     LLSSPETASSGLAMGLYSVLSTGAM"
BASE COUNT         1408 a         1186 c          916 g         1455 t
ORIGIN      
        1 atggaaatca gacaaataga agcggcctta accgatttcg acaaagctct gcatatagaa
       61 cctaacaatt catatgggta ttgcaatagg gcagtggctt ataactatat gcgtgacttc
      121 aacaaaagta tcagtgagag tactctggct gtagaaataa atcctcaact gccagaggca
      181 tatgtcgtta gaggaatcgc aaaagtacaa atgggagccc ttgcagctgc agacttagat
      241 ttcgaagttg ctgaatcctt aggctacaaa cggatagaca tagaagagcg tcttatagcg
      301 ttaagaaaag tctgactcct tctattttgt tttcaagaac tgccatttca ctcgcttcga
      361 ttttaatctt ggtctccggt gcgtgtagcc tatgggccgg gaaaagccta atccagtctg
      421 gtattttagt ctcatttttc gcccttctat cattgatagc cttcttggac catacagacc
      481 acaaaatccc caacaagatc acccttttcc ctataccaat cgtatttatt ttaagttacc
      541 tgtctccaga tatttttgaa ttttcggtca ctgaaacatg gtgggtgacc cctttagtag
      601 gaagtttaat tacaggatta atatttactt cgtgttacta cattcccaaa acccaactgg
      661 gtggcgggga tgtgaaatta gcgttgctga taggatgcat cagtggattc cccgtatgcc
      721 tggccagttt gtttttgggt ctattgattt tcatattaaa acaaacggtg atccaatact
      781 ctagaaaggt agtttctaca tccgcgttag ccccctatct ttctggaggg actatgattt
      841 caattttgat tgcacagcaa atctagaata ttatgcgctc agagtatatt gaacagtctg
      901 tggctcgata tattcggaaa ttcttcgtgt tatcggtata ctgccgatca cattcgtacc
      961 ttttccttct gccgaaatta tgttgacaga tccgcctact ctctctgtcc taagttttag
     1021 attgagtaac ccaaaatggc cggcctctgg tacattatct atcgatgatt gattgaaccc
     1081 gacaccattg tcttttacaa aaaacgatat gccttcctgt ttccagataa tctccacctg
     1141 tatcatctca ggattagcat gctctaccga atttaaaaca gactgtcgaa taatttgata
     1201 aagatcccta gctactggcc caggtaatcc cccttgatca atttgatttg aggtatatgc
     1261 tatttttatt gagaagggca ccacttcatt ctcaagccct tcaaaaaaga ttcgaagcga
     1321 ctcttccaac cccactatag caagatcatc atgtcctttc tctctcacta gctgtcttag
     1381 attgacgtca gcgtttttca ccatagtgac tagatgttca atatcggcag cgctctctgc
     1441 gtgtccatct agggttaatt ttaataaccc cagatgcatc aaaatagcac tcagctcact
     1501 cattgtttgg tcatgcagat cagcagctaa ttccttccgc atatcgtccc taccttgctc
     1561 aaccgcatca aactgctcga ttcttcttag tctgtatgcc atgatctggg atatcctaga
     1621 aataaactcg agagcctttt ctgggtccct ttctgatagc tgtgtgaaat cagccctatc
     1681 tacccggtac aacctacaag aggtccgagc tatcacacgg gccgtcctag ggtaggaatc
     1741 taagaatgcg atctctccaa ttactgatcc tgatttgatc catgctaccg gtatccattt
     1801 ctcattgacg gggacttcta ccaaagcctc tcctgagacg agcaaatcga tactggtggt
     1861 gatatcgcca tagtcaacaa ttgtctcttc ctcagaaaag aaaatttcct tgccgtaatc
     1921 agacaagtat tctctatatt tttcaatttc cttggcagtt aaaataggtt catccaaaga
     1981 acctgggtta tcaaaggagt tatcaggcat aaagatattc tcgatcttgg cctatcctaa
     2041 cgtaagagaa acacatagat ttaaaaaagc tatcgtggaa ttggcttgag cagacaaata
     2101 atcatcacct gaaacattta atttctcctc cactaatacc tgaatcactt ccacatccgc
     2161 cggaggaatc tctaagtcac tggcaatttc ctgaaggctc atacccttac accctctcga
     2221 tattacttcc aattccctgc ctgacaaatt ttcagaagct gaagaatgac gttcgtagta
     2281 accaattaat ttctctatca gtgcattgtc aagaattaga tgcccttgca taacttttct
     2341 tacaattctg attagttcac ctatatcatc caatgaattt tttagaacat atgctttcga
     2401 ttttgaatct gtctcaatta agtccttcac ataagaccag tcgtcatagg cagataccac
     2461 cacaacggaa acatcaggat tgttttccct aatggtttta ctcgctgaaa ttccgtcaag
     2521 tcgaggcatc ctaatatcta gtatcgctac atcaatctcc agctctgagc atttgtttac
     2581 agcttccagt ccgtcttcac aaactgatga aacgacaaaa tctgcctgcg tctcaagtac
     2641 ccgtttataa gctgtacgta tgaatggaga atcatctgct acaagaatat tggccattta
     2701 tatttggttt cccattactt cctagataat agttccgaaa cttctttaag ctctttagtg
     2761 agttggtcga tagaaactgt tccatactta tccagtaatt gccgaacctg ggggcgaact
     2821 tctatcttct cagcaaacaa atcccccatt tcagattctt cagcggaatc aacctcaata
     2881 tttgacgcta atacagaagg ttgttcttcc tctccggaat cctctctgct agggtcttct
     2941 tccgcctgag aattttgctc ttcattatca tcaatactgt ccaaagagac agtctggaaa
     3001 ttccttggtt tttgaagggt gtgtggttca attatagtgc tatcagcatc atcgggagta
     3061 tcattccctg ccgccgaaga ggttatcgta ttttgcagtg gtggcggggt tgcttccata
     3121 agagttggct caaacacatc ctcgtatatc tctgggtcca attcacttag atcccacagg
     3181 gcatcggaaa ctggtgctcc atcaacactt gtatcttccg tcaaagtttc ctccgaagaa
     3241 gatatgtctt ggctatttat ttctgatgtt gcctgtactt ccacgtcagc tttgagggtt
     3301 ttcattacat ttccgaactt atcataaatt gtcctacgaa ttgaatccat accatccatc
     3361 atcacatagc tcctgttgat aacaccgaat ataaccccat agccaatcca gaggaagcag
     3421 tttctggaga agaaagcaat gcagctgtta ctctaggtac agctaacatt atgacagccg
     3481 acgtcaaaca tgttacagca agaaaggtaa tgattttgag gttgttccca ccctcagaaa
     3541 attttggtgc taaggcattc gctactgtaa gtattgctac agccaatata atcgttgttt
     3601 gcaaggttgc tatatttccc atctggaata catcaacaga ggcacacaat tcctcaggtc
     3661 tgggcaactt tatgcctgct gcctcaacgc caggaggcac aaccacctgg tttaaacaat
     3721 tctctctatc tgatacccca actattgatc ctaacttact attgaaactt ataattattt
     3781 ccagaacaaa aacgagtatc agggccatca ctgaatgcat agtcatagtc agaaaagaaa
     3841 aggtggagga tgttagcttt cgcttggctc tcagttcgga gatgttctgg gcataatctg
     3901 aacaaatctc cccaatctgc cctggattac atccaacttc tactccatca acaaacattc
     3961 ctgcggtccg agaaactaat tcactaccag tttctctact aaacatctcc caacattcgg
     4021 aagatggcaa gcccgactct aatcgggttc tgaggcgttc cagtgggtct tttaaatatt
     4081 ggtaggaatt tgtgtcaata cgcctaagag cctcagttaa cgtaacacca cccgaaccgg
     4141 ctatgctccc tatagccctt aagaaaacag gaagttcctg atcgatgttg gataccctag
     4201 aagcatcccg catcgccaga aatgccgacg gtagcaaact tattcctgtc aacccaaaaa
     4261 tggaactgga tgcggtaacg gaactaactc ccatttccaa cagcaccaaa aaacccatga
     4321 cacctaatgc cagcccggtg ggtccacaaa tgatgaataa aaacttagcc attttccgat
     4381 caggggttgt tcctgaaaca gggtcgtatg taatcgtctc aaatggggcc actttataaa
     4441 ttagatacac ccctactgat gtgacaccac ccaatgtaaa caacattaaa taaatgaaag
     4501 atgcgctggt agaccccaac aggctagaaa cttgagaaac gaccataata agtgttacag
     4561 ctaccaatac agctgcataa gcatccgtcc atttacgaag gttttctacg tttctctcat
     4621 aatcgttact gtaatgttcc gcttcagccc ttgcctcctg aagaacaaaa tcagactctg
     4681 aactaccaga tgatatagag gcagaaaaac gtagtaataa gcttttaacc ctgggagctt
     4741 tcgctctctg ggcaaccgcc tggaaggccc ttgtgtattc cacccccatt gagcgagcca
     4801 tgaggttaat gtactcaaaa aatacggcgg ttaaatatct ttgtctcccg caatactcaa
     4861 gcagccggtc tctggtgaca ccacctcgag atagtatcgc catgtaagtc agctgagaga
     4921 ataaatcaaa cccaattact tggttatcag aagagacctt ctcta
//
LOCUS       DASCNS010000125         2753 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001064034, whole genome shotgun sequence.
ACCESSION   DASCNS010000125 DASCNS010000000
VERSION     DASCNS010000125.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001064034"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(126..959)
                     /locus_tag="QF838_04390"
     CDS             complement(126..959)
                     /locus_tag="QF838_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO59959.1"
                     /translation="MPSMPNAQIAQINGNSLAFYEWGEKGNPVLVCLHSHTNSAASWR
                     EFAEFASSKYHVFALDQRGHGNSEWANDGYARVKFVEDLSEFVKINEFETITLVGCSM
                     GGWHSILYTVTNQSVVDRIVMVDIAPEPSPERLQAPPAAPAPMEFQTIEDGYQWLRSG
                     NLLASDERLHEEAESRLKQTGSGTWTWKADLNGFDNPLPDMTSTELIDSYWSAIENIQ
                     CPIFEIRGAESGLVSDDTIDKMKTLGKDVTYIDVASAGHVVMVDQPGAFIEAVRGFVG
                     L"
     gene            1060..2154
                     /gene="ald"
                     /locus_tag="QF838_04395"
     CDS             1060..2154
                     /gene="ald"
                     /locus_tag="QF838_04395"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HJO59960.1"
                     /translation="MIIGLPKETKDQELRVGITPDGVGTLIKSGHQVLVEQNAGVGSG
                     ISNDSYEKVGAELIQKPEALFSASELIVKVKEFQLYECELLRPGLTSFSFLSLGANPK
                     LAEALLKSRVTAIAYETIEMTDGSLPILKPMSALTGRLAVDVATHYLKSPQGGTGKLL
                     SNISGVNPVKVVILGAGTAGFHSAELALNMGAEVTVLSRGKQRLGQLQESLNNHPKLK
                     IATSSSNKIEEAVELADVVIGAIRDAGGSVPKIVTKSMVQSMQKGSVIIDACIDQGGC
                     IETSRETTLSDPVYVEEGVIHYCVRNMPGAVPRTATEALVNVSLPYIKLLADKGVKDA
                     LATDTSLSIGLNVDNGRLVNPSVSKALGMS"
     gene            complement(2331..>2753)
                     /locus_tag="QF838_04400"
     CDS             complement(2331..>2753)
                     /locus_tag="QF838_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO59961.1"
                     /translation="GSAIAAAVETGKPVLAIEGDSAFGFSGMEIETICRYNLPVCVVV
                     FNNSGIYRGMDTNPTGGSDAATTVFVKDARYDKMIEAFGGVGVSVTSPDELTSAVNEA
                     MDSGRPTLINAVIDSSAGTESGRIGNLNPKSVIVAKKE"
BASE COUNT          821 a          614 c          557 g          761 t
ORIGIN      
        1 aggccggaaa agcctatcag agtccattga agatacttcc acccagctaa caggtaatca
       61 gacccatgtc gactcatttt ccagatggtt caccggttca taattaaaat taaaaggctc
      121 aatctctaaa gcccaacaaa tcctctgaca gcttctatga aggctccagg ttgatccacc
      181 atcactacat gtcctgcact tgccacatca atatatgtca cgtctttacc aagggtcttc
      241 atcttatcga tagtatcgtc gctcacaagg ccgctttctg cccctctaat ctcaaatatc
      301 gggcattgta tgttctctat ggcagaccag tagctatcaa ttaattcggt gctagtcata
      361 tctgggagtg gattatcgaa gccatttaag tccgctttcc atgtccaagt acctgaacct
      421 gtttgcttca acctagattc cgcttcttca tgtaagcgtt catctgaagc taaaaggttc
      481 ccagatctga gccattggta accatcttca atagtctgaa attccatggg ggcaggagct
      541 gcaggaggag cttgaagcct ttccggtgaa ggctccgggg ctatatcaac cataacaatc
      601 cgatctacaa cgctttgatt agtaactgtg tacagaatcg aatgccatcc ccccatcgag
      661 caccctacaa gcgtgatagt ttcaaattca tttattttga caaattcaga caggtcttct
      721 acaaatttaa ccctggcata gccgtcattt gcccactcac tatttccatg cccacgttgg
      781 tctagagcaa aaacgtgata tttagaagat gcgaactcag caaactctct ccaagaagca
      841 gcagaattag tatggctatg aagacaaact aaaactgggt tgcctttttc accccactca
      901 taaaaagcaa gtgaattccc attgatctgg gcgatttgtg cgtttggcat actaggcatg
      961 attggctcct aaatagaaag atggctataa aatagtactt cagctattaa atcagtccat
     1021 atatactggg tcaaatccat aacagtcaca gtaaatttta tgatcatagg actccctaaa
     1081 gaaaccaaag atcaagaact aagagtcggc ataacgcctg acggcgtagg cactttgata
     1141 aaaagtggtc atcaggtact ggttgaacaa aatgctggag tgggcagtgg tatatcaaat
     1201 gatagctatg agaaagtggg tgctgagtta attcaaaagc ctgaagcttt attctcagct
     1261 tcagaattaa ttgtaaaagt aaaagaattc caattatatg aatgcgaact tttacgccca
     1321 ggtcttacga gtttctcttt cctttcccta ggagccaacc caaaactagc tgaagccttg
     1381 ctgaaaagta gagtcacagc tatcgcatat gaaactattg aaatgactga cggatctcta
     1441 ccgattttaa agccaatgag cgctttgacc ggtagattag ctgtagatgt agcaactcat
     1501 tacctaaaaa gtccacaggg tggtacagga aaacttctaa gtaatatttc tggtgtaaat
     1561 cctgtaaaag tggtaatact tggtgccggt acggcgggat ttcattcagc tgaattagcg
     1621 ctaaatatgg gagcagaagt aacagtatta agccgaggaa aacagcgcct tggtcagctt
     1681 caagaatcgc taaacaatca ccctaaactg aaaatagcaa cgtcttcaag caacaaaata
     1741 gaggaggcgg ttgaattagc tgatgtcgtt attggagcaa ttcgggacgc gggaggatca
     1801 gtccccaaaa tagtcactaa atccatggtg caatcgatgc aaaaaggttc tgtaataatc
     1861 gatgcttgta tcgaccaagg tggatgtatc gaaactagtc gagagacaac actcagtgat
     1921 cccgtttatg tagaggaagg tgtgattcat tactgtgtac gcaatatgcc tggtgccgtc
     1981 cctcgaacag ctactgaggc cctcgttaac gtgtccctac cctacataaa attattagcg
     2041 gataaaggtg taaaagatgc cctcgctaca gatacttctc tttccatagg attgaatgta
     2101 gacaatgggc gactagtgaa cccgtcagta tctaaggcat tgggaatgag ttagatatta
     2161 acccttaaga aataattgat tatactttcc gaaatacttt ctctagggtc taacaaatat
     2221 cgggtatttt tcaaatattt gggatgggag cagatacggt tcgagggaag tagccaaagc
     2281 caattcaaaa tcgacattcc attaaggttc taaagtctat acttcattac ctattccttt
     2341 tttgctacga ttacgctttt gggattcaaa tttccgattc ttccactctc tgtcccagca
     2401 ctactatcta taacggcatt gattaatgta ggccttcctg aatccatggc ttcatttaca
     2461 gcactagtca gttcatctgg tgatgttacg cttaccccga ctccaccaaa cgcctctatc
     2521 attttgtcgt accttgcatc tttgacaaaa acagttgttg cagcatcgga gcctccggta
     2581 ggatttgtgt ccataccacg atatatacca ctgttattga aaacaacaac acatacagga
     2641 aggttgtagc ggcagatcgt ctcaatttcc atacctgaga aaccaaatgc gctgtcacct
     2701 tctatagcga gtactggttt ccctgtttca acggctgcag caattgcaga gcc
//
LOCUS       DASCNS010000126        13772 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001068905, whole genome shotgun sequence.
ACCESSION   DASCNS010000126 DASCNS010000000
VERSION     DASCNS010000126.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13772)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001068905"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="QF838_04405"
     CDS             complement(<1..186)
                     /locus_tag="QF838_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HJO59962.1"
                     /translation="MNIEKAVIPVAGLGTRFLPATLSIPKAMIPVLDQPSLHYCVAEA
                     INSGITKIIFVISENQKD"
     gene            complement(210..1469)
                     /locus_tag="QF838_04410"
     CDS             complement(210..1469)
                     /locus_tag="QF838_04410"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO59963.1"
                     /translation="MRNIPHFAALRHKDYAYTWGASALGGASAWTFLIASQWFVLERS
                     DQSAMVGWFTFASMIPFLVASPFGGFLADRIERRKLTIYTQILGVATVACAAALSLIG
                     WLELWHLCVLAFLAGSCRTTQEASIVSLITNLVPREDLLNAITLNSATRHGSRVIGMS
                     ILIISGVSQTITFGTFEFLIGSIIFGIGSIYLILKVKSNSIGEISSQTNLFKGIWEGV
                     NYVYTRRVVAVFIILVAFHCALAMSFDSILPGFTKNRLSSVDETMLAILVVSFGIGSA
                     AGTFLLAGVRNDKQKGLILLIMGVSSGISPLLLGFSFDISMAFASTLIMGATESVFMA
                     LTATYVQLVTPDRLRGRVTSLYILHAGGVMAFANLGYGYLADVYNAPNVLIVTGILFL
                     IFFAGMSLSDPILKSIYKGKDISALAS"
     gene            complement(1545..1617)
                     /locus_tag="QF838_04415"
     tRNA            complement(1545..1617)
                     /locus_tag="QF838_04415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1582..1584),aa:Ala,seq:cgc)
     gene            1809..2783
                     /locus_tag="QF838_04420"
     CDS             1809..2783
                     /locus_tag="QF838_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HJO59964.1"
                     /translation="MPYEELNKESLLWMYETMVMIRRFEEQSRREADAGKLRGMHSSI
                     GQEAVPTGICAHLNEKDYVLGTHRSHHHCIAKGLDLNEMMAELLGKSTGTGKGKGGTM
                     HIADIDRGMLGANGVVGSNIPVATGVALTAKVKGTDQVSVVFFGDGASSQGVLHESMN
                     LAGIWKLPVIFVCENNRYAEATPFEYSVAGGSVANRADGYGIPGVTVDGQSALDVYEV
                     GKEAVSRARMGEGPTLIEAQTYRYQGHFGADDPLGYRSKEEQEYYMSRDCILTLQKHI
                     LDGAYADETHLETIDKKCIEAVNIATEFANNSPFPNPEELVTDVYVTY"
     gene            2805..3833
                     /locus_tag="QF838_04425"
     CDS             2805..3833
                     /locus_tag="QF838_04425"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HJO59965.1"
                     /translation="MTQTQTVRQISFIQAINEALRQEMDRDPDVIVMGEDIAGGGERE
                     DFQDAWGGPMRLTKGLVNDFGRLRVRDTPISEAGFVGAGVGAAASGLRPVVDLMYVSF
                     YGVCADQITNNAAKMHYMFGGKVKVPLTIMTGIGAGTNSAAQHSETLYSIFTHFPGLK
                     CVVPSNPHNAKGLMTAAIRDDDPVIVFNNRQLMGLRFEDHVPEESYIVEIGKSEIKMQ
                     GEDITLLGIGYTTRVCMEAAGLLKERGYSVEVLDLLSLSPMDEDAILSSVKKTKKIVI
                     VDEDYPRCSIATDISALVAEEAFDYLDAPPKRVTPPHTQVPYSRPLEALFVPTTEKVV
                     AAALEVLE"
     gene            3844..5010
                     /locus_tag="QF838_04430"
     CDS             3844..5010
                     /locus_tag="QF838_04430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HJO59966.1"
                     /translation="MAIPIVMPRLGDFMTEGIVTKFTKTSGDNVSQGEVIAEIETEKV
                     NYDLEATNSGVFHPVVHQGATVLVDDVMGYLLEEGELPPEPPKEPIRTLTTENISQNV
                     DSPRPRTPNIKGNAIPSTPGARKLAAKLDIDLADIIPTGPRGRVVESDVRAIAEGSVS
                     SKPSLPPGLPRPISETPLEGMRKTIAAHMKNSLANTAQLSYFLDVDVTEAQNIRREQG
                     LGMTEVLIKATTEALIRVPALNSVLFNDVIVKFDRVNIAFAVALDDGLVVPVIRETNS
                     KNIREIAGEVKTLADAAKDGILGPDDFMGGTFTISILGSVDGFTPILNQSQVGLLGVG
                     RSLQKPIVKDGQIVIREMMTLSLTGDHQVVDGAVAASFFRRLQRLIESPSKLFA"
     gene            complement(5007..5753)
                     /locus_tag="QF838_04435"
     CDS             complement(5007..5753)
                     /locus_tag="QF838_04435"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HJO59967.1"
                     /translation="MRLNLTGPPRQPVFVYSIASINVILWILLEISGNSTNPSSLRKF
                     GAIDSYLIWSGEYWRLASAIFLHVGASHLAINSISLVIMGGMTERLYGHRNFITIFLV
                     TGIIGSTTSYLNIENGVVGAGASGAIFGCLGSLVSFFYIRKEVLGKSGKQTLNGLIIL
                     ALVNLAFGFVFPAVDNWAHIGGFIAGLGSGYFLAYHPRTTQHPHKLKIPYIGQKARLP
                     LLILFAVSIIISVLAIGNSMYEPAVVFPIF"
     gene            complement(5763..7076)
                     /gene="lysA"
                     /locus_tag="QF838_04440"
     CDS             complement(5763..7076)
                     /gene="lysA"
                     /locus_tag="QF838_04440"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HJO59968.1"
                     /translation="MNFSNIDVFPMGTKINSEGVLEIGGCDVLSLAKAHGTPLYVYDE
                     KTIRSMAQTFVGEFTSRYPKTSVAYASKAFLNLAMAKIANEENLSLDVVSGGEIAVAH
                     AAGFPSNKMYFHGNNKTPQELSEAIDLGVGTIVVDGFQELDILNNIAKSKNANQGIML
                     RLSPSVDAHTHAHTTTGILDVKFGFSIETGESSVAIKQALDSSHLILQGIHFHLGSPI
                     FELEPYSNAIDTVFDYLKPFLHQGLDLKKFSPGGGFAIGYLQRQLPPSIAGYADVITS
                     MLKTKCDDLGIDYPELIIEPGRSIVGRAGVAIYTVGVTKVIPNVRTYVSLDGGMGDNI
                     RPALYEAQYEAVAANKMSRTHFETVTLAGKYCETGDILVKDVSLPILDSGDLVAIPAS
                     GAYCIAMSSNYNMNPRPEIIMVSDGGSRIIKRRETYQDLMALDFS"
     gene            complement(7091..7927)
                     /locus_tag="QF838_04445"
     CDS             complement(7091..7927)
                     /locus_tag="QF838_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO59969.1"
                     /translation="MKITDRITKIVVGENEPDPFKYGGVYPPNVFLVSGKNRSVYIDT
                     AHGKPEEIQEHISVWESQGKPEIAGIILTHRHSDHIGGAQELGEQMGAPVISTSVEKI
                     AIEKESSATVGLTPKDGEKLDLGDTTLYFIHTPGHTMGSLCIYLEEDKVLFTGDNILG
                     LGTTVISPDEGDMTSYIKSLHKMLNYDIEKICPGHGPEIDTPIEKIHELIEHRHQREI
                     QMLELITSGKDSMDDIFNAIYKDIHPGLHNTARRQIQAHINKLVEEKRIEQTGNIYRI
                     VQ"
     gene            complement(7944..8606)
                     /locus_tag="QF838_04450"
     CDS             complement(7944..8606)
                     /locus_tag="QF838_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59970.1"
                     /translation="MQAEVKWIHPGPQPNGLQADSDGLWAIDQGNDHIYKLSYIDGSI
                     IENFDTETSKSSGITKGGGFLWVASTYTAELYQLNMDGSTAAVFDTPGKGIVTQYGSS
                     ENPQITGAHGMEWVDQNNMWVVVPPAQQAFLLDPVSMEVKRSIPTPGVRPHGLFIDNG
                     NMWLADTQECKIYKINPDNGEVQSEIHIPEPEIHGMTIYDGDIWFCCAETRKVCTVKL
                     PG"
     gene            8798..10210
                     /locus_tag="QF838_04455"
     CDS             8798..10210
                     /locus_tag="QF838_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015948.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HJO59971.1"
                     /translation="MSDSTVYYMDAHSESTETALAAKMITVFDAAGLDEMIKPNDIVA
                     IKVHCGEWNNSAYLRPLYARQLADRIKELGGRPFVCDTTTSSYSPWGSRTSEIDILLT
                     AERNGYSSATLGCPFICADGFIGTSDFVVDIPEGYLLKEAYVAQAIAAADALIALTHF
                     KGHSMGVIGGALKNLGIGAQSKRGKFNVHMGGHPTYGLGAAGVFHPENFKGKANTPDW
                     EILEDCCPFNLYHINENDELEWEGDKCANCLGCFGVMGPRGLMDIPPVQFDAVDAAIA
                     DACLGVEKAVGRDKVGYINMALDVSPRCDCANHADVPIVPHLGVFASKDAVAIDMACV
                     DKAREAEGIKGSAAEMMEAHHAGDKKFEAAAATFHGQSEVTSINTGHEIGLGSREYEL
                     IECEPDSPERFRFPYDTRPSRQRFGERFEKFQPFPYDKHDGKGFNRLDVVDLDKVKHH
                     YEDSPEITEVSESIHADGDD"
     gene            10311..10634
                     /locus_tag="QF838_04460"
     CDS             10311..10634
                     /locus_tag="QF838_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO59972.1"
                     /translation="MPQSGQEMLDETISACKAIADGLGAQNQDWENSVAEIVEKFEEV
                     SGTFFFKTMPSVPATRTAMRDAASALELKNASEWDAMGTALETLIASSQNLIEKAGMK
                     GTTLT"
     gene            10631..11506
                     /locus_tag="QF838_04465"
     CDS             10631..11506
                     /locus_tag="QF838_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024852.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO59973.1"
                     /translation="MNEPVLITGGAGSVGRQLAGMFLAEGRPVRIFDLPFMDFSGLED
                     ESNVEVVKGDITDKDSVNEAVRNVGGILHLAALLPPTSERNRDKTFAVNVEGTRNITE
                     ALKSHGSKATLVFTSSISTYGDTSKESDPIKITQPQNAIDIYAESKIAGEKVLIGSGV
                     NFVILRIASIAVPAFLEPPEPWPFTSDQRVEMVHRDDVADAIKSSVDTPEAVGNVFNI
                     AGGSTWRLAGKNYVEDFFNFMGAPVEMAVYREEPGWNDWYDTVESQKILNYQNRSYGF
                     YSGEMKAIVEEMMAE"
     gene            11531..12373
                     /gene="larE"
                     /locus_tag="QF838_04470"
     CDS             11531..12373
                     /gene="larE"
                     /locus_tag="QF838_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HJO59974.1"
                     /translation="MVQVTQKSVEEKRQQLVNIINDMGSVIVAYSGGVDSAFLAAVAH
                     EVLSGKALSVTANSPSLAPSELEDAIALATEQGIHYEIIETKETEREDYQANNPDRCF
                     FCKDELYSHLIKFADQRGYAVITNGTNVDDLGDYRPGLEAAKQYGVRSPLVEADLTKQ
                     DIRDLSKKMGLPTWDKPAQACLSSRIPYGTMVTVEALTRIAKAEHFLRSKGFKQLRVR
                     HHDTVARIEIEPKDFLALLDDSVRDEITEYFKSVGYSYVTMDMNGFRSGSLNEILTTF
                     KKKK"
     gene            12460..>13772
                     /locus_tag="QF838_04475"
     CDS             12460..>13772
                     /locus_tag="QF838_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HJO59975.1"
                     /translation="MSGIVQKEIEKYVARTPGSYKRQKEAEKFLPGGSSRGTAYFEPY
                     PHFIESGHEHYIVDVDGNEYLDFMINATSLILGHANANVAEVIKDQADKGVAFSGPTD
                     SQISLAKILTDRIPSVDTVRFTNSGTEGTLMAIRAARQFTGREKIVKIEGGYHGSHEY
                     VAVSVYPSKSSLDPDKTTAIPEYSKQPDSVLEEVIVVPYNDIEETARMIEENAETVAC
                     VIMEPIVSSFGYTPANLEYLEFVREITTRLGIILIYDEVQSFRVAPGGAQELFGVVPD
                     MTTLGKIIGGGMPVGAFGGRRDIMDLFDPTKGGAEIGHAGTFNANPVTMRAGEVVMNA
                     LTPDVYEAMNNLGEKLRNQLRAVFDELEVDAQVTGVGSLFGIHFTQDSIRNYRDVVNA
                     DSDMKRAFYFGMLNEGILLQSGAAGALNVFSDENHVNQLVNSARKV"
BASE COUNT         4029 a         2657 c         3238 g         3848 t
ORIGIN      
        1 gtccttttga ttttcagata tcacaaatat aatttttgtt attcctgaat tgatggcctc
       61 cgcgacacag tagtgaagtg aaggttgatc caagacaggg atcatggcct ttggaatcga
      121 cagcgtggca ggtaagaacc gagttcctag cccagctact gggattacgg ctttttctat
      181 gttcataaag aaggcctttt caataatgat taacttgcca atgctgagat atctttacct
      241 ttgtaaatcg attttagtat tgggtcactt aaactcatgc cagcaaagaa aataaggaat
      301 aaaatgcccg ttactataag aacattcggg gcattgtaga catcagctag gtatccgtag
      361 cctaaatttg caaatgccat aactcctcca gcatgaagta tgtaaaggct cgtaactcgc
      421 cccctcagcc tgtctggagt aacaagttga acataggttg ctgttaaggc cataaaaacc
      481 gattcagttg ctcccattat tagtgtggaa gcgaatgcca ttgatatatc gaaggagaat
      541 ccaagtaata aaggagagat accactagag acacccatta tcagcaatat caaacctttc
      601 tgcttatcgt ttctcacacc ggctaataga aaagttcctg cagctgagcc gattccgaat
      661 gacacgacga gaatggccag catagtttca tcaactgatg atagacggtt ttttgtaaag
      721 ccgggtaaga tagaatcgaa agacatagca agagcacagt gaaaagcaac aagtattata
      781 aaaaccgcaa ctactctacg cgtatagacg taattgacgc cttcccaaat ccccttaaag
      841 agatttgttt ggcttgaaat ttcaccaata gagttgcttt tgactttcaa aatcaggtaa
      901 atggaaccaa tcccaaaaat aatactacct atcaaaaact cgaaagttcc aaaagtgata
      961 gtttgtgata ccccggaaat tattaaaatt gacattccta ttactctcga accatgtctg
     1021 gtggctgagt tcagcgtgat tgcgttaagg agatcttctc tcggtaccaa atttgttatt
     1081 aaagaaacaa tggaagcctc ttgtgtagtt cgacaacttc ccgccaaaaa agcgagaaca
     1141 cataggtgcc ataactcgag ccatcctata agtgacagtg cagcggcaca ggcaacagtg
     1201 gctactccta gaatttgagt gtagattgta agttttcttc tctcgattcg atcagctaaa
     1261 aatcccccaa atggagaggc aaccaaaaag ggaatcatgg aagcaaacgt gaaccatcca
     1321 accatcgcgc tctggtcaga tcgttccagc acaaaccatt gactggcaat aaggaaggtc
     1381 catgcactag ctccacctaa ggcgctggcc ccccaggtat acgcataatc tttgtgccgc
     1441 aaggcggcga aatgaggtat atttcgcaag aattactcct ggaagattcc catcatggct
     1501 gagggaataa attccaattc aggtgatgaa aaatgattta tttgtggaga tagggggaat
     1561 cgaaccccca acctcggcat tgcgaacgcc gcgctctccc gtttgagcta tatccccttt
     1621 tcactataag tagacgaaat cgtatcaacg tgactatttt ggtgtcaatc accttttgaa
     1681 gatacagtaa ccaatcagaa aaatactgta aattgtggtt cccacaaact tgcttggcat
     1741 actgttataa tcgcgccaat tttaaagcct atattagtta ttgactagcg tttttgggag
     1801 gtgttgaaat gccttacgaa gaacttaaca aagaatctct gctttggatg tatgagacca
     1861 tggtgatgat tcgccgtttc gaggaacaat ctaggagaga agcggatgct ggaaaattac
     1921 ggggtatgca ttcttcaata gggcaagaag cagttccaac aggcatatgt gctcacctga
     1981 atgaaaaaga ttatgtcctt ggcacccata gaagccacca tcattgtatt gccaaaggtc
     2041 ttgacctaaa tgaaatgatg gctgaactgt taggcaagtc tacgggtacg ggaaaaggaa
     2101 aaggtggcac tatgcatata gcagatatag acaggggtat gcttggtgct aatggagttg
     2161 taggttcaaa tattcctgta gcaactggtg tggcattgac tgcgaaggta aaaggaacag
     2221 atcaggtcag cgttgttttt tttggagatg gtgcctcaag tcagggagtt ttgcatgaat
     2281 ctatgaatct tgcaggcatt tggaaattac cggttatttt cgtatgtgaa aataatcgct
     2341 acgctgaagc gactcccttt gaatattcag tggcaggtgg ctcagtcgcc aatcgagctg
     2401 atgggtatgg gattccagga gtaacggtag atgggcagtc agccttggat gtttatgagg
     2461 tgggtaagga ggcagtgtca agagctcgta tgggtgaagg tcccacgttg atcgaggctc
     2521 agacgtacag atatcaaggt cattttggtg ccgatgatcc tctaggttat aggagtaaag
     2581 aagagcaaga atattatatg tcacgtgact gtatcctaac gttacaaaaa catattttag
     2641 atggtgctta tgctgatgaa acccacctgg aaactataga taagaaatgt atagaagcag
     2701 ttaatatagc caccgaattc gctaataaca gtccgtttcc aaaccctgaa gaattggtca
     2761 cagacgtata cgtgacctac taactcaaat aggagataaa ttaaatgaca cagacacaaa
     2821 cagtcagaca aataagtttc atacaagcca ttaacgaggc tctaaggcaa gaaatggaca
     2881 gagatcctga tgttattgtc atgggagagg atattgcagg gggtggagaa cgggaagatt
     2941 tccaagatgc ttggggtgga ccgatgagat tgaccaaagg gttggtaaat gattttggtc
     3001 gtttgagggt gagagatacc ccaatatcag aagcaggatt cgttggggcg ggagtaggag
     3061 ccgctgcttc aggtttgaga ccggttgtgg atttaatgta tgtgagtttt tacggcgtat
     3121 gtgctgatca gatcactaat aatgccgcta aaatgcacta tatgtttggt ggaaaagtta
     3181 aggttccatt aaccataatg acaggaatag gggccggcac gaattcagcc gctcaacatt
     3241 ccgaaaccct ctattccatc ttcactcact ttcctggtct taaatgtgtt gtcccttcaa
     3301 acccacataa tgctaaaggg ctgatgaccg cagccattag agatgatgac ccagtaattg
     3361 tgttcaataa tcggcagctt atgggattaa gatttgaaga ccatgttcca gaggaatcct
     3421 atattgttga aattggtaaa tcagaaatta aaatgcaagg ggaagacata accttgttgg
     3481 gtattggtta cactacacgg gtgtgtatgg aggccgcggg cctgttgaaa gagcggggtt
     3541 attcagttga agtgttagat ttactatctc tttctcctat ggatgaagat gcgatacttt
     3601 cctcggtaaa aaagactaag aaaatcgtta ttgtagatga agattatcca agatgtagca
     3661 ttgccactga tatctctgct ttagtggctg aagaagcttt tgattatttg gatgcaccac
     3721 cgaaaagggt aactcctcca cacactcaag tgccatacag ccgacccctg gaagccctat
     3781 tcgtacctac cacggagaag gttgtagccg ctgcattgga agtactggaa taggagattc
     3841 aatatggcaa ttccgatagt catgccgagg ttgggtgact tcatgacaga aggcatcgtg
     3901 acgaaattta ccaaaacatc aggggataat gtatctcaag gtgaggttat agccgaaatt
     3961 gaaaccgaaa aggtaaatta tgacttagaa gcaactaatt cgggtgtgtt tcacccagtt
     4021 gttcatcaag gggcaacagt actggtagac gatgtcatgg gttacttgct tgaggaagga
     4081 gaacttccgc cagaacctcc gaaggaaccc attaggacgt tgacgaccga gaatatttct
     4141 caaaatgttg attctccgag accaagaaca ccaaacataa aagggaatgc tataccttct
     4201 actccaggtg ctcggaaact tgcagctaaa ttggatatag accttgctga cataatcccg
     4261 actggtccga gaggccgggt tgtcgaatca gatgtcagag ccatagcaga gggttcggtc
     4321 tcatcaaaac cttcattgcc tccaggattg ccaagaccga tatctgaaac ccctttagaa
     4381 ggtatgagga aaaccattgc agcccatatg aaaaatagtt tggcaaatac cgcccagttg
     4441 tcctattttc ttgatgtaga tgtgactgag gctcagaata taaggcgtga gcaaggtctg
     4501 ggtatgactg aggttttaat aaaagctact actgaggcgt tgattcgagt tcccgcccta
     4561 aatagcgttc tatttaatga tgtaattgtg aaatttgatc gggtgaatat agcttttgcg
     4621 gtggcattgg acgacggctt ggttgtcccg gtgattcgag aaactaactc aaaaaatatc
     4681 agagaaatag ctggggaagt aaaaactctt gctgacgctg ctaaagacgg tattttggga
     4741 ccggatgatt tcatgggagg gactttcacg atatcaatac tcggatcggt agatgggttt
     4801 acccctatat tgaaccaatc acaggtaggg ttgctcggtg tcggtagatc acttcagaag
     4861 cctattgtca aagatggcca aattgtcata agagagatga tgactttgag tctgactggc
     4921 gaccatcaag ttgtggatgg ggctgtggct gcaagttttt tcaggagact tcagcgcttg
     4981 attgaaagcc cttcaaagtt atttgcttaa aaaataggga ataccactgc tggttcgtac
     5041 atggaattgc caattgccag cacggaaata attattgaaa ccgcaaaaag tatgagtagg
     5101 ggcaacctgg ctttttgccc aatgtagggt attttcaatt tatgtgggtg ttgggttgtt
     5161 cttggatgat aagctaaaaa gtaccccgaa cctaatccag caataaatcc gccaatgtgt
     5221 gcccaattat cgacagctgg gaatacgaat ccaaaggcta gattcactaa agctaatata
     5281 attaaaccat ttaaagtttg cttgccagat ttccctaaaa cctctttacg tatgtaaaaa
     5341 aaagaaacta agctacccaa acaaccaaaa atggccccgc ttgccccagc gccgacgacc
     5401 ccattttcaa tgttcagata gctggtggta ctaccaatta tgcctgtcac tagaaaaatg
     5461 gtaatgaagt ttcggtgccc gtacagccgc tctgtcatgc cccccatgat gactaagcta
     5521 atggaattta ttgctagatg ggaggcacca acgtgaagga aaatcgcgct tgctagtcgc
     5581 caatattcac ccgaccagat taaataggag tcaattgccc caaatttgcg aaggctagag
     5641 ggatttgtac tattcccgga gatttccagt aatatccaga gaattacatt gatagatgct
     5701 attgagtaga caaaaactgg ttgtctggga ggtcctgtta agtttaatcg cacagtgatt
     5761 gactatgaga agtccaaagc cattaaatct tggtaagttt ctcttctttt tattatccgg
     5821 gaacccccat cggaaaccat tataatttcg ggcctagggt tcatgttgta attacttgac
     5881 atggctatgc aataggcccc tgaagctggt attgcaacta agtctcctga atccaatatc
     5941 ggcagtgaaa catctttcac gagaatatca cctgtttcac aatactttcc ggccaaagtc
     6001 acggtctcaa aatgagtgcg ggacatttta tttgctgcaa cagcttcata ttgagcctca
     6061 tacaaagcag gtctaatgtt atcccccatt cctccatcta acgatacata ggttcgaaca
     6121 tttggaatta cttttgttac acccactgtg tatatagcta cccctgctcg cccaacgatc
     6181 gacctgcctg gttctattat cagttcaggg tagtcaattc ctaaatcgtc gcatttggtt
     6241 ttcagcatgg atgtaataac gtccgcatag ccggctatag atggtggtaa ttgtcgctgt
     6301 aaatatccga ttgcaaagcc gccgccgggg ctaaattttt tcaaatctaa accttgatgt
     6361 aaaaatggtt ttaaataatc aaagacagta tcaatggcat tggaatatgg ttctaattcg
     6421 aagattggcg aaccaagatg gaaatggatt ccttgaagta taaggtggct agaatctaga
     6481 gcctgtttaa tagctacact gctttctccg gtctctattg agaagccgaa tttaacgtcc
     6541 aatataccag tcgttgtatg tgcgtgggtg tgggcatcaa cagaaggaga caatctgagc
     6601 attatccctt gattagcatt tttagatttg gcaatattat ttaagatgtc tagttcttga
     6661 aacccatcga ccacgatggt cccgactcct agatcaatgg cttcagaaag ttcttgggga
     6721 gttttattat ttccatggaa atacattttg ttgctaggaa accctgcagc atgagccact
     6781 gctatttctc ctccagaaac cacgtcgagg gaaagatttt cttcattggc tatttttgcc
     6841 attgcaagat tgagaaacgc ttttgaggca taagcaaccg aggttttagg atatctagaa
     6901 gtaaattcac ccacaaaagt ttgggccatg cttcttattg ttttttcgtc gtacacatag
     6961 agtggggtgc cgtgagcttt tgcgagagaa agcacgtcgc atccacctat ttcaaggact
     7021 ccttcagagt tgatttttgt gcccatagga aaaacgtcga tatttgagaa attcacagag
     7081 tgtctcctgt ttactgaact attcggtaaa tgttaccggt ttgttcgatt cttttctctt
     7141 cgaccagttt atttatatgg gcctgtatct gtcttcttgc tgtattatga agaccggggt
     7201 gtatgtcttt gtatattgca ttgaatatat cgtccataga atcttttccg gaagtaatta
     7261 actctaacat ttgtatttca cgttggtgtc tgtgctcaat taattcgtga attttttcta
     7321 tcggagtatc tatctctggt ccatgccccg gacatatttt ttcaatgtca taattcaaca
     7381 ttttgtgtag ggatttgata taagaggtca tatcgccctc gtcgggtgaa atgactgttg
     7441 tccccaagcc taaaatgtta tcccctgtga aaaggacttt atcttcttcc agatagatac
     7501 atagacttcc catagtatgg ccaggagtgt ggataaaata taaggtggta tcccctaaat
     7561 ccagtttttc tccatcctta ggagttaggc ctactgtagc tgacgattct ttttcaatag
     7621 caattttttc tactgatgta gatataactg gagctcccat ttgctcacct aattcttggg
     7681 ctccaccaat gtggtcactg tgcctatgag tcaatattat tccagctatt tccggtttcc
     7741 cttgtgattc ccaaactgaa atatgttctt ggatttcttc cggtttgcca tgtgcagtgt
     7801 ctatatagac agatctgttt tttccactga ccagaaatac atttggagga tatactcctc
     7861 cgtatttgaa gggatctggc tcgttctcgc caactactat tttggttatt ctgtctgtga
     7921 ttttcatttt ttgtcctttt ttcctaacct ggtaatttca cagtacaaac ctttcgagtt
     7981 tcagcacagc agaaccatat gtcaccatcg taaatggtca taccgtgtat ttcaggttca
     8041 ggaatatgaa tttcgctttg gacttcgcca ttgtctgggt tgattttata tattttgcat
     8101 tcctgtgtgt cagcaagcca catatttcca ttatctatga acagcccgtg aggtcttact
     8161 ccaggggtag gaatagaccg cttcacttcc atacttacgg gatccaacaa gaatgcttgt
     8221 tgggctggtg ggacaacaac ccacatatta ttttggtcta cccactccat accatgcgct
     8281 ccagttattt gagggttttc tgaagagcca tattgggtaa ctatgccttt tccaggtgtg
     8341 tcaaatacag ctgccgtact gccgtccata ttcaattggt aaagctcagc agtgtatgta
     8401 gaggcaaccc aaagaaatcc ccctcctttt gttattccgc tggatttaga ggtttccgta
     8461 tcaaaatttt ctatgattga accgtcgata taggacaatt tataaatatg atcgttacct
     8521 tgatcgatcg cccaaagccc atcagaatcc gcttgtaaac catttggctg agggccaggg
     8581 tgaatccatt ttacttctgc ctgcattggt gtttccttat ctaggtgtgg atttgaattt
     8641 tatgctagtt atcttcctac ttaatgaggt attttagtat tgattagaaa gtttgttaaa
     8701 ttattgacat gaataggaag gaatgctaat ctcaatttat acatgattag ctagggaaat
     8761 tgtacctgtt tggtcaaaaa aaggagtgga gggaaatatg tctgattcga cagtttatta
     8821 catggacgca cattcagaat ctactgagac tgcgttggca gcaaaaatga tcacagtttt
     8881 tgatgccgca ggtttagatg agatgataaa gccaaacgat attgtggcta taaaagtcca
     8941 ctgcggagaa tggaataact ctgcatattt aagacctctt tatgctcgac aattggccga
     9001 taggataaaa gagttgggtg gtcgcccatt tgtctgcgac acaactactt cctcctactc
     9061 accatggggc tcgaggactt cggagataga tatattgctg acagcagaaa ggaacggtta
     9121 tagctcagcc acattgggct gccctttcat atgtgctgat ggttttatcg gtactagtga
     9181 ttttgtcgtt gacataccag agggatacct ccttaaagag gcttatgttg ctcaagccat
     9241 cgcagctgcc gatgcgctaa ttgcactaac ccacttcaaa ggacatagca tgggagtcat
     9301 tggtggtgcc ctaaagaatc tgggtattgg ggcacaatcc aagagaggta aattcaatgt
     9361 ccatatgggt ggccacccaa cctatggatt aggggcagct ggagttttcc atccggagaa
     9421 cttcaaagga aaggcaaata cgccagattg ggaaattttg gaagattgct gtcctttcaa
     9481 tctctatcac ataaacgaaa atgatgaatt ggaatgggaa ggcgataagt gtgcaaattg
     9541 cctgggttgt ttcggagtca tggggcctag gggattgatg gatattcctc ctgttcagtt
     9601 tgatgctgtt gacgcagcta ttgccgatgc ctgtctaggg gtagagaaag cagtaggtag
     9661 agataaagta ggttatataa atatggcctt ggatgtatcg cctcgctgcg actgtgcaaa
     9721 tcacgccgat gtccctattg ttccccatct tggcgttttc gctagtaagg atgctgtagc
     9781 aattgatatg gcttgtgtag acaaggctag ggaagctgaa ggcataaagg gatctgcggc
     9841 tgaaatgatg gaagctcatc atgccggaga taaaaaattc gaggctgctg ctgcaacatt
     9901 ccatggccaa agtgaggtta ccagcattaa cactgggcat gagataggac taggtagtag
     9961 ggaatatgaa ctaattgaat gtgaaccaga tagcccagag cgcttcaggt tcccttacga
    10021 tacaaggcca agcaggcaaa ggttcggcga aagatttgaa aagttccagc cgtttcctta
    10081 cgacaagcac gatgggaaag gcttcaaccg attggatgtt gttgacctgg acaaagtgaa
    10141 gcatcactat gaggattcac cggaaataac tgaagtaagt gaatcaattc atgctgatgg
    10201 cgacgactaa ggcacttcac tgtctttagg agttgaatac gaaaagggcg accacctagt
    10261 ggccgccctt taatatttag ataatattaa tattaggagg gattctgtaa atgccgcaat
    10321 caggacaaga aatgcttgat gaaacaatat ccgcttgtaa agctattgca gatggacttg
    10381 gtgctcaaaa tcaagattgg gaaaattcag ttgctgaaat agtggagaaa tttgaagagg
    10441 taagtggaac ttttttcttc aaaacaatgc caagtgttcc agcaactcgt acagccatga
    10501 gagacgctgc atcggccttg gaattaaaaa atgctagtga atgggatgct atgggtactg
    10561 ctctggaaac cctgatagct agttctcaaa atctgattga gaaagctggt atgaaaggaa
    10621 cgaccctaac ttgaatgagc cagtgctgat aacaggtgga gctgggagtg taggaagaca
    10681 actggctggt atgtttttgg ctgaaggaag gcctgttcgg atatttgacc tgccattcat
    10741 ggatttttca ggattggaag atgaatccaa tgtggaggta gttaaaggtg acatcactga
    10801 taaagactcc gtaaacgagg ccgtgcgcaa tgtgggagga attctccatt tggctgcact
    10861 tctcccgccg acaagtgaac gaaatagaga taagacattt gcggtgaacg tggaaggcac
    10921 taggaatata actgaggctt tgaagtctca tggatccaag gctactttag ttttcacctc
    10981 ttcgataagt acatacggcg atacttctaa agagtccgac ccaattaaga ttacacagcc
    11041 acaaaatgca atagatatat acgcagaaag taaaatcgca ggggaaaagg ttcttattgg
    11101 atcaggtgtt aattttgtga tattgcggat agccagtatc gcggtaccgg cctttttaga
    11161 gcctcccgaa ccttggccct ttacgtctga tcaaagagtt gaaatggttc atagggatga
    11221 tgtggctgat gctataaaat cttcagtgga caccccagag gctgttggaa atgtatttaa
    11281 cattgccgga ggttctactt ggcgtttagc tgggaaaaat tatgttgaag atttctttaa
    11341 ttttatggga gctccagttg agatggcagt ataccgagaa gaaccaggtt ggaatgattg
    11401 gtatgatact gtagagtccc agaagatact gaattatcag aataggtctt atggttttta
    11461 ttcaggtgag atgaaggcaa tcgtggaaga aatgatggct gagtagcatc taagtaaagc
    11521 gaggattggt atggtgcagg taactcaaaa atctgttgag gaaaaaagac agcaactcgt
    11581 aaatatcata aatgatatgg gcagcgtgat tgtcgcttac tcaggtggcg tagacagtgc
    11641 ctttttagcg gctgtcgccc acgaggtttt gagtggcaag gccctgtcag taaccgctaa
    11701 ttcacccagc ctggctccat ccgaactcga ggatgctata gcccttgcga cagaacaggg
    11761 aattcattac gaaattattg aaacaaaaga aactgagcgt gaagactatc aggcaaacaa
    11821 tccggataga tgtttttttt gtaaagatga gttgtattcc cacctaatca aatttgcaga
    11881 tcaacgaggc tacgcagtaa ttaccaacgg gactaatgtg gatgatttgg gagactatag
    11941 gcctggtctt gaggctgcaa agcagtatgg agtgagaagt cctttggtgg aggcggatct
    12001 taccaaacaa gatataagag atttatctaa aaaaatggga ttgcctacgt gggataagcc
    12061 tgcacaagct tgcctttctt cgcgtatacc gtatggaact atggtgacgg tggaagctct
    12121 taccaggata gcgaaagcag aacactttct cagatccaaa ggttttaaac agttgagggt
    12181 aaggcatcac gatactgtag ctcgtataga aatcgaaccc aaggattttt tagccttgct
    12241 agatgattcc gtcagagatg aaattacaga atatttcaaa tcagttgggt attcatacgt
    12301 aacaatggat atgaacgggt ttagatccgg tagtcttaac gagattttga ccacttttaa
    12361 gaaaaagaaa taaatctgct ttaggtatcc taggcgattt attgggcaat tgagataaac
    12421 ctgctcgctc aaacaacttt agctattgag ggtataaaca tgtctggtat cgtccaaaaa
    12481 gagatagaaa agtatgtggc aaggactcct gggtcctaca aaaggcaaaa agaggcagag
    12541 aaatttctgc caggtggcag ttcaagaggg actgcttatt tcgaacccta cccccatttt
    12601 attgaaagtg ggcatgaaca ttatattgtt gatgtcgatg gaaatgagta tctagatttt
    12661 atgattaatg caacgagcct aatacttggt cacgcaaacg ccaacgtggc tgaagtaatc
    12721 aaggatcaag cagacaaagg cgtagcgttt agcggaccca ctgattcgca aataagtctg
    12781 gccaagatac taacagaccg cataccatcg gttgacacgg tacgattcac taattccggt
    12841 accgaaggta ccttgatggc catcagggct gcgcggcaat ttacgggtag agagaaaatc
    12901 gtaaaaattg aaggcggata ccacggatca catgaatatg ttgcagtcag tgtatatccc
    12961 agtaaaagta gtttggaccc tgataaaact actgccatac cagagtattc gaagcaacct
    13021 gattcagttt tagaggaagt gatagtcgta ccatataacg acattgaaga gacagcccgg
    13081 atgattgaag aaaatgcaga aacggtggca tgtgtaatta tggaaccgat agtctcctca
    13141 tttgggtata ctccagcgaa cttagagtac ttggagtttg tcagagaaat tacgacgaga
    13201 ctgggcatta tattaattta tgatgaagta caaagtttca gagttgctcc aggaggagca
    13261 caggaattgt ttggagttgt tccagatatg accactttgg gcaaaataat aggtggtggt
    13321 atgcctgtgg gagcctttgg agggcggaga gacataatgg acttatttga tcccacaaaa
    13381 gggggagcgg aaataggtca cgctgggact tttaatgcca atccagttac gatgagggca
    13441 ggtgaagtgg tcatgaatgc cttaacgcct gatgtttatg aagctatgaa caacttaggt
    13501 gaaaaactaa ggaatcaact cagggctgta tttgatgagt tggaagtgga tgcgcaagtg
    13561 accggggttg ggtcgctttt tggcatacac ttcacccagg acagcatacg caattataga
    13621 gatgtggtta atgctgattc agatatgaag cgggcttttt acttcggcat gctgaatgaa
    13681 ggaattttgt tgcagtcggg agcagcagga gccttaaacg tgttttctga tgaaaatcat
    13741 gttaaccaat tagtgaactc tgcaagaaag gt
//
LOCUS       DASCNS010000127         5019 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001071456, whole genome shotgun sequence.
ACCESSION   DASCNS010000127 DASCNS010000000
VERSION     DASCNS010000127.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001071456"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            155..2749
                     /locus_tag="QF838_04480"
     CDS             155..2749
                     /locus_tag="QF838_04480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HJO59976.1"
                     /translation="MTTENRLLLPQSVTPDNYQVELEPNFETFTFNGKVIIKASVNEN
                     TDSVTLNSAELEITAVSAVSQGHNLEVSTISTDPDHETLTITFDNTIKKGESNLQLQI
                     SFVGELNDRLLGFYRSQYTDINGNEKYLAATQFESTDARRAFPCWDEPSTKATFDVLL
                     VIPGEMKAVSNMPIASESQGRDGQKTIVFDTTPVMSTYLLAFIIGELGCIEQSTDSGT
                     LMRVWTTSGKEEQGRFALQTSVDLLNYYNDYFGIPFPLPKLDHLAIPDFAAGAMENWG
                     AITYREVALLVDPDNSSAGTRQIVAAIISHEMAHMWFGDLVTMKWWNDLWLNESFASW
                     MGDKAVDAIHPEWDMWTQFLTADTASAFSLDGLSNSHPIEQEVNNPAEIGQLFDAISY
                     SKGGSILRMLEDFIGASDFQKGIRAYLTDNSYGNAETQDLWSALEVSSGKPVGKVMDS
                     WVKQTGFPMISADRAQDSKNIKLSQTRFLYDHIEDKQDNKEKTSWFVPIKIGSNLDDN
                     LQTLLMESRDIKIQLETNQKGNVSWIKLNPNQTGFYRTKYTESDLENLENAIVAGELN
                     PRDRLGVQGDCYALVRAGYTPVEHFLNLATGYSNESDASVLSDLATGLRGIENLISEL
                     SCHPEYQIFCRNIFSAISEKIGWEKRLGDGHLDALLRSTSLGNSGHYGDKSVLEEARI
                     KYDNHVSGRQLIHPDIRTVVFNLAAQEGDKVVYDQMWVLEKASNLQEEKVRLHSALSN
                     FKDRSLLQETLKKSLSEDIRVQDSIRVIVTVAGSVLGRSLAWDFVRDNWKEIDRRYGD
                     GGFALMRLVSIVSGFTTSERLKEVELFFEENPTPAAERTIRQAMERIRLNQAWLSKYS
                     GQISDFLR"
     gene            complement(2754..4088)
                     /locus_tag="QF838_04485"
     CDS             complement(2754..4088)
                     /locus_tag="QF838_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJO59977.1"
                     /translation="MDSNSLKLKCWEIIDSRADWLVNISKHILQNPEPGFHEFETSKF
                     VSQKLQELGLPHQKGIALTGIKGIIDGGLPGPTIAIIGELDSLRVAGHSHANPETDAA
                     HACGHHCQIGMMLGVVAAFQEIAKSSKTRGKLAFMALPAEEFIDVEYRYGLHKEGQIE
                     FMSGKQEFLNIGAFNDVDMAVMAHTSKIEEGKFSIGGTSNGHVVKYVRFIGKASHAGG
                     SPHEGINALQASIVSLNALNTQRETFKNEDTIRLHGIMTKGGVSVSSIPAEIKYEGRV
                     RGGTTAAIADANLKMDRCLRAGALALGCELEIITLPGYMPIKNNPHMSDIFISNAASL
                     VGDDHVIQKSPTHNSGGSTDMGDLSQIMPVIHPYTGVATGTGHGDDYLVQDYTQGVVN
                     PAKALAATIVDLLCREDNPANKILSEFQPDYSIKSYLKLQRSRLTEEVYKPK"
     gene            complement(4111..>5019)
                     /locus_tag="QF838_04490"
     CDS             complement(4111..>5019)
                     /locus_tag="QF838_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019307.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HJO59978.1"
                     /translation="PAEEYIEVEYRDGLRKGGEIEFLGGKPEFVRLGHFDDVDMAMMT
                     HTSVPSNNCKISFGGTNNGLVAKSIKFSGVASHAGGAPHMGVNALNAAMVAMSAIHAQ
                     RETYRDQDTIRIHPIITKGGVAVSSVPADVRMETYVRGASIEAFLSASEKVDRALRAG
                     AMAVGGSVAITTLPGYLPIQSNETFLDLYQGNAAELVGSENLGRLTHRTGSTDMGDIS
                     QLMPVIHPYVEAASGNGHGIDYVIEDYELAVLTGAKAMAMTVVDLLTEGASNASKIVS
                     ENEPPLTKSSYLSLLRSMTKEETFKE"
BASE COUNT         1482 a         1109 c         1031 g         1397 t
ORIGIN      
        1 cacttgataa tgcactacaa agaaagtact cagaggaaaa taattacctc ggttcagctg
       61 atcaaaggga aggaccattg gcattttcag aaaaaagaaa accaaattgg atcggggcct
      121 aaggaaacat taaataaagg catttgcact cactatgaca acagaaaaca gattgctact
      181 tccccaaagc gttaccccag acaattacca agttgaatta gaacctaact ttgaaacttt
      241 cactttcaat ggaaaagtca tcatcaaagc tagcgttaat gaaaacacag attcggtaac
      301 gcttaactca gccgaacttg agatcactgc tgtatcagca gtttctcaag gtcacaattt
      361 agaagtgtcc actatttcca ccgacccaga tcacgaaacc ctcaccatca ctttcgacaa
      421 taccatcaaa aaaggcgaat ccaacttaca gttacaaatt tcatttgttg gtgagctcaa
      481 tgataggtta cttggttttt acagaagcca atacactgat attaatggaa atgaaaaata
      541 cttggcagcc actcaatttg agtccacgga cgcaaggaga gctttcccat gttgggacga
      601 accctctaca aaagcaacct ttgacgtctt actggtcata ccaggtgaaa tgaaggccgt
      661 atcaaatatg cctattgctt ccgaatctca aggaagagat ggtcaaaaaa ccatagtttt
      721 tgatactacc ccggttatgt ccacttattt acttgctttc attattgggg aattaggatg
      781 cattgaacaa tcgaccgata gcggaacatt aatgcgagtt tggaccacat ccggaaaaga
      841 ggaacaagga cggtttgcat tgcaaacttc tgtggatttg cttaactatt ataatgacta
      901 ttttggtatt ccgtttcctc tgcccaaatt agaccatctg gccatccctg actttgcagc
      961 cggtgccatg gagaattggg gagccatcac atatcgagag gtagccttgt tggtcgaccc
     1021 tgacaatagt tcagcgggta caagacaaat agtggctgca ataatttccc acgaaatggc
     1081 ccatatgtgg tttggtgatt tggtaacgat gaagtggtgg aatgatttat ggctcaacga
     1141 gagcttcgct tcatggatgg gagataaagc agtagacgct atccatcctg aatgggatat
     1201 gtggacgcag tttttaactg cagacactgc ctcagctttt tctctagacg gtctctcaaa
     1261 ttcacatccg atagaacaag aggtaaataa tccggcagaa attggacagt tgtttgatgc
     1321 gatcagttat agcaaagggg gctcgatttt acggatgctg gaggacttca taggagcttc
     1381 agatttccaa aagggtatac gagcctactt aacagacaac tcctacggaa acgcagaaac
     1441 tcaagattta tggagcgctc ttgaagtttc ttctggtaaa ccagttggta aggtaatgga
     1501 ttcctgggtt aaacaaacag gatttcctat gatttcagcc gacagagccc aagattccaa
     1561 aaacataaag ctttcccaaa caagattttt atatgaccac atagaagata agcaggacaa
     1621 caaagagaaa acatcttggt ttgtcccaat aaagatcggc agcaacctgg acgataatct
     1681 tcaaactctg ttaatggaat cccgtgatat aaaaatacaa ttggagacca atcaaaaggg
     1741 caatgtctca tggattaaat taaacccaaa ccaaacagga ttttatagaa ctaaatacac
     1801 tgaatcggat ctagaaaatt tagaaaatgc aatcgttgcc ggggaactta atcctagaga
     1861 tagactaggg gtgcaaggag attgttacgc tcttgtcagg gctgggtata caccagtgga
     1921 acatttcctt aatctagcca cagggtattc caatgagtct gatgcttccg tactttctga
     1981 ccttgcaact gggcttaggg gtattgaaaa cttaatttcc gaattaagtt gccaccctga
     2041 atatcaaatt ttctgtagga acatattctc ggctataagc gaaaaaatag gatgggaaaa
     2101 acgattaggt gatggccatt tagacgcact tttaagaagc acgtctcttg gaaattctgg
     2161 acactatggt gacaaatctg tccttgaaga agctcgcatc aaatatgaca atcatgtatc
     2221 tggacggcaa ctcatacacc ctgacatacg tacagttgtt ttcaacttag cggcccaaga
     2281 aggcgataaa gtagtttatg accagatgtg ggttttggaa aaagcctcga atcttcagga
     2341 agagaaggtt agactccatt cggccttatc taattttaag gatagatctt tgttgcagga
     2401 aactttgaaa aaatcactaa gcgaagatat ccgcgtacaa gactcgataa gggtaattgt
     2461 gacagtagca ggctcagtat taggaagatc attagcctgg gattttgtac gagacaattg
     2521 gaaagaaatt gaccggagat acggtgacgg gggattcgca ttgatgcgcc ttgtatcaat
     2581 agtttccggg tttacaacca gcgaaagatt gaaagaagtg gaactcttct ttgaagagaa
     2641 tccaactcca gcggccgaga ggactataag acaggccatg gaaagaatac gattgaacca
     2701 agcttggctt tcaaaatact ctggccaaat atcggatttt ttgaggtaga accctattta
     2761 ggtttataga cttcttcagt tagtctcgat ctttgaagtt taaggtatga tttgattgag
     2821 tagtcgggct gaaattcact caatatctta tttgccggat tgtcttcacg gcaaagcaaa
     2881 tcaactatcg tagccgcgag cgccttcgct ggattcacca caccttgagt gtaatcttgc
     2941 actaaatagt catccccgtg gccagtgccc gtagcgactc ctgtgtatgg atgaattaca
     3001 ggcatgattt gactgagatc gcccatatca gtgctgccac cagaattgtg agttggggat
     3061 ttttggatca catgatcatc ccctaccaaa gaggcagcgt tgctaataaa aatatctgac
     3121 atatgaggat tatttttgat tggcatatat cctggtaatg tgattatctc cagctcacag
     3181 cctaaggcaa gtgcccccgc tctcaaacac ctgtccattt tcaaattagc atctgcaatt
     3241 gctgcagttg tgccacctct tactctccct tcatatttaa tttcagcagg tatcgaacta
     3301 acggaaacgc cccctttggt cataatgccg tgaagtctta tggtatcctc gtttttgaag
     3361 gtctctcttt gggtatttaa agcgtttaac gaaacaattg aagcctgcaa ggcattaata
     3421 ccctcatgag gtgagcctcc ggcgtgagaa gccttaccta taaatcggac gtatttcacc
     3481 acatgtccgt tacttgtacc tccgattgaa aattttcctt cttcaatttt ggaggtgtgt
     3541 gccatgacag ccatatccac atcgttgaat gctcctatat tcaaaaattc ttgtttccca
     3601 gacatgaatt caatttgacc ttccttatga aggccatatc tatactcaac gtcaataaac
     3661 tcctcggcag gaagagccat aaatgccagc ttaccccggg ttttggaact cttggcaatt
     3721 tcctggaaag cagcaacaac ccctagcatc atcccaattt gacaatgatg gccacatgcg
     3781 tgggcagcgt cggtttccgg attggcatgt gaatgtcctg caaccctcaa agaatccaat
     3841 tctccgatta tggcaatagt tggtccaggc agtccaccat cgataattcc cttgattccc
     3901 gtgagggcaa ttcctttttg gtgcggcagg ccaagttctt gcagcttttg gctcacaaat
     3961 ttggatgttt cgaactcatg gaagcctggt tcaggatttt gaagaatatg tttcgatata
     4021 tttaccaacc agtcagcgcg actatcgata atctcccaac acttcaattt aagggaatta
     4081 gaatccattc ctatcttctc tgatacacaa ttattcttta aaagtttctt ctttagtcat
     4141 gcttcgcaaa agggaaaggt atgaagattt ggtgagtgga ggctcattct cggacactat
     4201 tttagaggca ttagaagctc cttctgtcaa taggtctaca acggtcattg ccattgcctt
     4261 tgctccagtt aggactgcca actcataatc ctctatcacg tagtcaatac catgcccgtt
     4321 accagatgcg gcttcaacgt atggatgtat cactggcatt agctgagata tatcccccat
     4381 gtcagttgac ccagtccgat gggtaagtcg acccaggttt tcagaaccaa ctaattcagc
     4441 cgcattccct tgataaagat ctaaaaaagt ctcgttactt tgaatcggaa gatatcccgg
     4501 cagagttgta atagccactg atcctccaac agccatcgca cctgccctta aagccctatc
     4561 aactttttcg gatgcagata aaaacgcctc tattgatgcg cccctaacgt aggtttccat
     4621 cctgacatcg gctggcactg aacttacagc cactccccct tttgtgataa tcggatgtat
     4681 tcttatcgta tcttgatccc tataagtttc cctttgggca tgtatagcag acatagcaac
     4741 catagcagca tttagagcat tgactcccat atgaggtgct cccccggcat gagaagcaac
     4801 tcctgaaaat ttgatcgatt ttgcaaccaa cccattgttt gtcccaccaa atgatatttt
     4861 gcaattattt gatggcaccg aggtatgagt catcatcgcc atatcaacat cgtcaaaatg
     4921 tccgagtctt acaaattctg gcttaccacc caaaaattct atttccccac ctttccttaa
     4981 gccatccctg tattcgactt caatatactc ttcggctgg
//
LOCUS       DASCNS010000128         1966 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001072897, whole genome shotgun sequence.
ACCESSION   DASCNS010000128 DASCNS010000000
VERSION     DASCNS010000128.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001072897"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="QF838_04495"
     CDS             <1..506
                     /locus_tag="QF838_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HJO59979.1"
                     /translation="EVDGIQVTKYLRSLDIDIPICILSARDEVSDRISGLEAGADDYV
                     VKPFSFEELLARIHALLRRRSLNDSKVVSVGELKVDPMRRTADYYGHSLELTKKEFDL
                     IHVLANNPSLVMSREQLLNRVWGYDFDVDTNVVDVFIGYLRKKLEIDGQKRIIQTVRG
                     VGFVLNT"
     gene            503..1930
                     /locus_tag="QF838_04500"
     CDS             503..1930
                     /locus_tag="QF838_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HJO59980.1"
                     /translation="MSFRFLITLLVFFVTTLIVILSGAVINRLTEEDLRHRIDSRLSW
                     QVQAVSSEGSLSTILKLRRFVQRSEVSDASLDNLFDVQIPTRIFLNSDLFLYTDGFPE
                     ILTTSNNGYSNVSVDGQEWRVLTKTIQIPLVLRTSKFDSIGIQAAVSRDFVSTTMQDF
                     RKTFISVGIIAILLSALVTWVLCGVLLKPLKRLQIYAENINDSSDLSIRIPVEHLYSL
                     SEVKVLSISLNSMISRLDSSSTRTEKALETSRGFASNLAHELRTPLTSMKMNLELLEK
                     HGEIPADERRNILNEVIIQQDRLFSTLESLRLLARGDLSEVNVFEEVDLAPLINELSA
                     RQIANHGGGNIDIQLPDPPPLILGWREGLTVLFDNLLQNAYVHSNVSPEQLEIVIGVS
                     VEEKWVHINVDDNGVGVPEHERKTVLERFKRGSKNYGQGSGLGLSLVTQQVEIHSGRI
                     EISESSMGGTRVELALPILMDPELD"
BASE COUNT          575 a          350 c          448 g          593 t
ORIGIN      
        1 cggaagtgga tggtatccag gtcaccaagt accttcgaag tctagatata gatattccca
       61 tatgtatttt gtctgctaga gacgaggtat cggacagaat ttcaggttta gaggcggggg
      121 ctgacgatta tgtggttaaa cctttctcgt ttgaggaatt gctggctcga attcacgcat
      181 tacttagaag acgttcctta aatgattcta aagttgtatc ggtgggcgaa ttgaaggtcg
      241 atcctatgcg caggactgca gattactatg gacattcctt ggaattgacc aaaaaggaat
      301 ttgatctgat tcacgtattg gcaaataacc caagcctggt aatgagtagg gagcagctgt
      361 tgaatagagt gtggggatat gatttcgatg tagatacaaa tgttgtagat gttttcattg
      421 gatatctacg caaaaaactg gaaattgatg ggcaaaaaag aattatacag acagtcaggg
      481 gagtggggtt cgtactgaat acgtgagctt tcgattttta ataactctac ttgtattttt
      541 tgtaaccacc ttaattgtta ttctatctgg tgcagtcata aatcgactca ccgaagaaga
      601 tcttagacat cggatagatt caaggttaag ctggcaggtg caggccgtct cctcggaagg
      661 ctcactttct acaattttga aattacgacg ttttgtacaa cgctctgaag tttctgatgc
      721 ctcattagat aatttgtttg atgtgcaaat accaactcgt atttttttga acagcgattt
      781 gtttctttat acggatggtt ttccagaaat attgaccacc tctaacaacg gatattccaa
      841 tgtttcagtg gatggtcaag aatggagagt tctgacaaaa accatacaga ttccacttgt
      901 actccgcaca tccaaatttg acagtatagg tattcaagct gccgtctctc gagactttgt
      961 tagcacgacg atgcaagatt ttaggaaaac tttcatttcc gtaggcataa tagccattct
     1021 attatcagcc ttagtgactt gggtactttg tggagttctt ctcaagcctt tgaaacggct
     1081 ccagatttat gcggaaaata taaatgattc ttcagatttg tctatccgca tacctgtaga
     1141 gcatttgtat tcactctctg aagtcaaagt tctgagtata agtttaaatt ccatgatttc
     1201 cagattggat tccagttcta cccgtaccga aaaagcgctc gagacatcaa ggggttttgc
     1261 gtctaatctg gctcatgagc taagaactcc gttaaccagt atgaaaatga atttggaact
     1321 attggaaaag catggagaaa taccggccga tgaaagaagg aatatattaa acgaggtaat
     1381 aatccaacag gacagacttt tttctacctt ggaatcttta agattactcg ctcgtggtga
     1441 tctttctgaa gtaaatgtct ttgaggaagt ggatttggcc cccttaatca atgaattatc
     1501 tgccaggcaa atagctaatc atggtggtgg caatattgat attcaattac cggacccacc
     1561 gccacttata ttgggctgga gagaaggttt gactgtcctg ttcgataact tactccaaaa
     1621 tgcatacgta cattcgaatg tttcaccaga acagttggaa attgtgattg gtgtgtcagt
     1681 tgaagaaaaa tgggtacaca tcaatgtgga tgacaacggg gtaggtgtcc cagaacatga
     1741 aagaaaaacg gtgttggaac gattcaaaag agggtctaaa aactatggtc agggctcggg
     1801 ccttgggctt tctttggtca cacaacaggt tgagatacat tccggaagga tagaaatctc
     1861 ggagagttca atgggtggaa cgagagtaga attagcgtta cccatcctca tggatcctga
     1921 attagattag ctttgatctt ctgaggaaat ctagtaatag ttttct
//
LOCUS       DASCNS010000129        11298 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001073317, whole genome shotgun sequence.
ACCESSION   DASCNS010000129 DASCNS010000000
VERSION     DASCNS010000129.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001073317"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="QF838_04505"
     CDS             <1..331
                     /locus_tag="QF838_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HJO59981.1"
                     /translation="LCAGTAAALSRYVLTNSDLNPFQTTEIRLLGSIIALLPFLRQRL
                     TDSLKKLPLNNKFSLLYATFFGTNIGILLQQNVFKLLPIGLGWTLLSTSPVMALFFAR
                     VEGEELI"
     gene            465..917
                     /locus_tag="QF838_04510"
     CDS             465..917
                     /locus_tag="QF838_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HJO59982.1"
                     /translation="MYDLEDLYQEIVMDHNRRPRNFGEIKNPTNSADGFNPLCGDQIK
                     LQLHLKDGLVADVAFSGVGCAISKSSASMMTEELKGKTLDHVENLFGAFRKMITRKPG
                     DSYDADLLGDLEILQGISRYPARIKCATLAWHTLHSAIHDENEVVSTK"
     gene            943..1371
                     /locus_tag="QF838_04515"
     CDS             943..1371
                     /locus_tag="QF838_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HJO59983.1"
                     /translation="MHVPIKVDYGVRALVDLAIYGSDGNSLRSSEISERTAIPNAYLA
                     QVLNSLKRAGFVKSIRGPNGGHELAVDPDTIHLNSIMEVLDSTDNLVNCMDDDNFCVH
                     SPACAQKEIWRTVEEAIHGVLFSITVADLARRTQVIKLQI"
     gene            1416..2657
                     /locus_tag="QF838_04520"
     CDS             1416..2657
                     /locus_tag="QF838_04520"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HJO59984.1"
                     /translation="MFDVEKIRGDFPILSKKVYGNPLVYLDNAATSQKPRQVIDSLVD
                     YYENYNANVHRGVHTLSMEATDRFEEAREKVARFINAESSDLVMWTRNASESLNLIAY
                     SWGEDNVHEGDEILLTPMEHHSNLVPWQELARRKNAVIKFIPMLENGTLNMGEVDDLI
                     TERTVLVSAVHMSNALGTINPVRELGEKAHRQGAKILVDGAQSVPHMPTNVQELDCDF
                     LVFSGHKMLGPTGIGALYVKQDILNTMEPFLTGGEMVLEVSYQEASWADLPMRFEAGT
                     PNIADSIGLGSAVDYLNNLGMENVRKHEKDLISYALNRFQEAGLEGMDLFGPDDPEIR
                     GGVFSFNTVDVHPHDMGTFLDRMGIAVRTGHHCAMPLIRSLGVAATVRASFYLYNTER
                     EVDILVDGVTEALRYFRDGSE"
     gene            2644..3090
                     /locus_tag="QF838_04525"
     CDS             2644..3090
                     /locus_tag="QF838_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HJO59985.1"
                     /translation="MDQNNIDDIYRDRIILDHCRNPRNTSPIEYFDLKGDAVNPFCGD
                     EIHFQIVLKGENSIERIQFSGEGCAINMASGSLLSEAVTGLTLKEAGQLAIRFVESMR
                     KPIGEKVEGSFIGELSALTSVRNFPVRVKCALLAWSALEDAISERK"
     gene            complement(3092..4828)
                     /locus_tag="QF838_04530"
     CDS             complement(3092..4828)
                     /locus_tag="QF838_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO59986.1"
                     /translation="MKHLVHLAVTFGFIAGFVSFACADQSKLEQEFFENQPSVDAVIT
                     PLPTIGIDLSPKPTPSPIQNMVTPTPLSVENGSPDNSLEPAIPINKIPPIFEGKRGGT
                     LRLISKHYFDSLDPHENYSGAHSTWGIGIIYQRLLKFSNGPDITLPSKSTECDACSSW
                     QMVDNKTFIFDVDTNIKWVSEEADDMNQLLPEDIKFSLDRQMAKNQEHSNRFHMVRSV
                     DVKSENQIQINLNVPDADFFISLADGRSKLLSKSDINDPGKVESAPPIGSGPWRLSKL
                     VDDSYSSVENVFSAPNPPYLDSIEFHIITDPQARLSAYSVGLIDVYNIENYSTELDIA
                     LENPEPGLGFEIAFNTAKPPFKDAHTRVLARSSINPNEIIQKAWHNYAFFSLGFVTNH
                     SSWIPVRSDWESYFYPSQHPETLKEIIPISIATSDFGSKYKESAEIASKQLEAVGFEP
                     IINFVNRRQYAEKIWGEGDFDVLMGPTFPQSSTNSFVIPVLHSEGKWNTSNHRDMRLD
                     ELIEEQSQEYNLEKRSEIINEMNRHLLEKSYRFMPATQKSLWAWTEDLKNMHPNFSGL
                     EYSHWERVWLDR"
     gene            4914..5711
                     /locus_tag="QF838_04535"
     CDS             4914..5711
                     /locus_tag="QF838_04535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HJO59987.1"
                     /translation="MLIDTHVHLDAFPDCDIEAILDRGHDVGVGFVICAGTTVDSSIR
                     SVDLSRRFHRFFSGVGVHPMDISKPLTTGDYQTLAELASNEPKVIVMSEIGLDYMEGM
                     PDRAWQFTAFREQISIARAQNLPIVFHSREAHQDCLRVLKEEKGYEVGGIMHYFQGSL
                     EEAHQVIDLGFYISIARPIFRLPHLQEVVAKIPIENIVIETDSAPQPFKKNRDNWTEP
                     RYLRPITEKIAELKDMDIDIVEASLFQNTKKLLSSQWEIVTQSVPNS"
     gene            complement(5708..6253)
                     /locus_tag="QF838_04540"
     CDS             complement(5708..6253)
                     /locus_tag="QF838_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016764.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HJO59988.1"
                     /translation="MIDNLVKAAILNKNIYPEFKSRPELVIQSLGTVAIAAFSSAIYL
                     HYKGTVINPESDFARLQMMAVAFSTIFVGMMMWSFINNTLCGLMGGDAEFRDTIRSTG
                     IAYGPGVGLILATIPIVGDYILLITLVWLLVAVTIGVKTTHNITLLKAFFPSAIGWFM
                     AWILLPWLMIIGPYFTNPMLD"
     gene            6369..6611
                     /locus_tag="QF838_04545"
     CDS             6369..6611
                     /locus_tag="QF838_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HJO59989.1"
                     /translation="MLTQGAYLTVIGMGTSFLLLLILITSILVTKYVSNKIDPPYVPP
                     TEEQKQELARKAKAAAAAFAFLSAKGRDPKIDSEVK"
     gene            6642..7802
                     /locus_tag="QF838_04550"
     CDS             6642..7802
                     /locus_tag="QF838_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HJO59990.1"
                     /translation="MIEQLLDFIRTTGLFNMGWRDAVMIFVGLIFIYLAIKKDWEPYE
                     LLPIGLGIIAANLPLTGLITPPTSDSLNQEAGIFGIFFHYGLSFWNILPPIIFLGIGA
                     LTDFGPVIANPKTLLLGAAAQIGIFVAFWGALISGSLGMNFGIEEAASIGIIGGADGP
                     TTIFLSARLAPEILGITAVIAYSYMAAVAFIQPPLMKLFTTKKERQIVMRPLREVSKL
                     EKLIFPNVALIAIILVVPKSAPLIAMFMIGNLFRESGAVPRLTKSASNEILNIATIFL
                     MITVGTQLTADRVFDWQTMVILILGLVAFSCGTIGGILFAKIMNLFLNEKINPLIGSA
                     GVSAVPMAARISHHLGQQANPNNFLLPHAMGPNVAGVIGSAVIAGVFISLVA"
     gene            7807..8205
                     /locus_tag="QF838_04555"
     CDS             7807..8205
                     /locus_tag="QF838_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HJO59991.1"
                     /translation="MKIRVEEKWYNIEVDSISSDSAVVLVDGERFEVKLEDVEKTAEL
                     TEEISGPVSNSVGMRTDNYKEFKSPMPGTIISILVQTGDDVSQGQDICVLESMKMQQT
                     LKSDMAGTIMGTSVSQGDQILDGDVIFLIR"
     gene            complement(8226..8765)
                     /locus_tag="QF838_04560"
     CDS             complement(8226..8765)
                     /locus_tag="QF838_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012725.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJO59992.1"
                     /translation="MTIIVCSLLSCDNNSNGATLLVPTQEPVPTANKISRETEITPTI
                     TPTNPISASPTPEIEALFLYNRGLNLLRSEQFIESVNSFSTLIKRMPDLAIAYKSRGA
                     AYYHLERYELAKTDLEKALTLDEDLGGTHLYLGLIHKKNGNLVEAKKELQMAIHLIHP
                     IRETEELLIAESSLANLEH"
     gene            8893..9408
                     /locus_tag="QF838_04565"
     CDS             8893..9408
                     /locus_tag="QF838_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HJO59993.1"
                     /translation="MLIKTLEDKTPLIHPTAFVSEAAYIVGDVEIGEGSSIWPGTVIR
                     GDRGKVKIGKYTNIQDNSVVHGDADVEIGDYVTVGHRVVCHSQYIGNQSLIGNGAILN
                     DGVIIGENSVVGSGAMLLENMDIPKRSIVVGMPGRVRGEINKKHLDLQVELADIYSDL
                     AKRYKNEGALE"
     gene            complement(9419..10192)
                     /locus_tag="QF838_04570"
     CDS             complement(9419..10192)
                     /locus_tag="QF838_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO59994.1"
                     /translation="MVINLHNVSFKYGNMNVIQEITTGFKPGELVSLVGPNGAGKTTL
                     LKLLSGTLKPSSGEIYIYDQLSYDLTPKNRAIWVSMVPQNPKLPDEISISDFVMLGRN
                     PHLGLLQWESKEDFNIARESIFLTEIDYLSKRKLGEISGGEKQRAMLALAITQQSPIM
                     LLDEPTSNLDISHQMKVMKIIKKIHEQKSEFGVTVLAIHDLNIAAQFSDRILLFSKGS
                     IAADGTPKEVLTKNNIKSIYGSEVTIITHPEHGTPVILP"
     gene            complement(10182..11261)
                     /locus_tag="QF838_04575"
     CDS             complement(10182..11261)
                     /locus_tag="QF838_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013219.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HJO59995.1"
                     /translation="MKSPANTSNQDLVLAYKYPWNKITLGIVVILICAVSGVAVGSVH
                     IPITSVFLILFSKLDLFNESGELTSAWHTIIWNIRFPRVVLALLVGASLGISGATYQG
                     LFKNPLADPYLIGVASGASLGATIVFLSGIPFTYGYFSLLPVASFCGGITAVFIAYLI
                     SIKSGNTHLSTLLLAGLAIGAFATAITGILMLRSDPDLRPLLSWLMGGFIRAQWSHSI
                     LIMPYMIVGVSFILSHSRILNTMQLEGYYAESLGVNIEKSKRILIVSATLVTAAAVSF
                     SGLIGFVGLIAPHIIRLLWGTDYRTLLPLSGLTGAAFLILADLTARTIVSPIELPVGI
                     ITALVGSPFFVYVLLRTKGSTIDGY"
BASE COUNT         3318 a         2068 c         2536 g         3376 t
ORIGIN      
        1 attatgcgct ggcacagcag cagctttatc aaggtatgta ctcacaaact ctgacttgaa
       61 cccattccaa actacagaaa ttcgattgct cggtagcatt attgctctcc ttcctttcct
      121 aaggcaaagg ttgactgatt cccttaaaaa gttgccttta aataataaat ttagtctttt
      181 atatgcaaca ttttttggga caaatatagg gatcttgttg cagcaaaatg tctttaaatt
      241 attgcctatt ggattaggtt ggacactttt aagtacttcg ccagtaatgg cacttttctt
      301 tgcaagagtg gagggagagg aattgatcta gaagctgcta gccgattgat aatttatgga
      361 ttcgctgggg agataattga taaggtggaa atacctgagc tgaggtcata tcttgtggaa
      421 tcttttctta gttctttacc tagtcatagg tttgagtttt aacgatgtat gaccttgaag
      481 atttatacca agaaatcgtg atggaccaca ataggagacc tcgtaatttt ggagagatta
      541 aaaacccgac taattctgcg gatggattca atcctttatg tggggatcaa ataaaacttc
      601 agctacacct aaaagatggt ttagtagctg atgtagcgtt cagtggggtt ggctgtgcaa
      661 tatccaaatc ttccgcgtcg atgatgactg aggaattgaa agggaagaca ttagatcacg
      721 ttgaaaactt gtttggagca ttcagaaaaa tgattaccag gaaacctgga gactcatatg
      781 atgcggattt attgggtgac ttagaaattt tgcagggaat ttcccgatat ccggcgagaa
      841 taaaatgcgc aacccttgct tggcacactt tgcattcagc aattcatgat gaaaatgagg
      901 tcgtatctac aaagtagttc ttattgtaga gtgaggtgaa ctatgcatgt accgattaaa
      961 gtcgattatg gtgtgagagc actggtggat ttagcaatat atggatctga cgggaattca
     1021 ttgagatcct cagaaatttc tgagaggact gcgataccaa atgcatatct ggctcaagtg
     1081 cttaactctt taaaaagagc agggtttgtc aaaagtatta gaggcccgaa cggaggccat
     1141 gaattggcgg ttgatccaga cacaatacac ttgaattcta taatggaagt gttggatagt
     1201 acggacaatt tagtcaattg catggatgac gataatttct gcgttcattc tccggcatgt
     1261 gcacagaaag aaatatggag gacggttgaa gaagcaatac acggggtttt attctcaata
     1321 accgtagccg atttagctag aagaacccaa gtcattaagt tgcaaattta aaacgatacc
     1381 aaaatgtcat ttctgcttgg gagtaaattt atttaatgtt cgatgttgaa aaaataaggg
     1441 gcgattttcc gattctatct aaaaaggtct acggtaaccc cttagtttat ctagataatg
     1501 ctgcgactag tcaaaaacct cgccaggtaa tcgattctct agttgattac tacgaaaatt
     1561 ataatgctaa cgtgcacaga ggtgtccata cattaagcat ggaggccaca gatagatttg
     1621 aagaagcaag ggaaaaggtg gctagattta ttaatgctga gtcctctgac ctagttatgt
     1681 ggactaggaa tgcctccgag agtttaaacc tcatcgccta ttcatggggc gaagataatg
     1741 tacatgaagg ggatgaaatc ttgttgaccc caatggaaca tcacagtaat ttggttccat
     1801 ggcaagaact cgctcggagg aaaaatgcag tcattaagtt tatacccatg ctagaaaacg
     1861 gaaccttaaa tatgggtgaa gttgatgacc taatcacaga gaggacggtt ctcgtctcgg
     1921 cggttcatat gtcaaacgct ttaggcacta ttaatccagt gagagaattg ggtgaaaaag
     1981 cccatagaca gggggcaaaa attctggttg atggagccca aagtgtgcct catatgccga
     2041 ctaatgttca ggaattggat tgtgattttc tggtattttc agggcataaa atgttgggac
     2101 ccacaggaat aggagctttg tatgtgaagc aggatatatt aaatactatg gaacctttcc
     2161 tcacaggagg tgagatggta ctagaggtat cttatcaaga ggcatcttgg gcagatctac
     2221 caatgagatt tgaggccgga actcctaaca tagctgattc tattgggctt ggttctgctg
     2281 tcgattatct aaataaccta ggaatggaga atgttaggaa gcacgagaag gatttaatca
     2341 gttacgcatt aaataggttt caggaggcag gtttagaagg catggacctg tttgggccgg
     2401 acgatcctga gataaggggt ggagtttttt cctttaatac ggtagatgtt catccccatg
     2461 atatgggaac atttcttgat aggatgggga tcgctgtaag aacagggcat cactgtgcta
     2521 tgcctttgat acggagttta ggggtggcag ccactgtaag ggcaagcttt tacttgtata
     2581 acacagagag ggaagtggat attttggtag acggggtaac agaagcgctg aggtatttca
     2641 gggatggatc agaataatat cgatgatatt tacagggata gaattatcct ggatcattgc
     2701 agaaaccccc gaaacacttc accaattgag tattttgacc ttaaaggaga tgctgtaaat
     2761 cccttttgcg gtgacgaaat ccattttcaa attgttttaa agggtgaaaa ttcaattgaa
     2821 cggattcaat tctctggtga gggatgcgct ataaatatgg cttctgggtc attgttgtca
     2881 gaggcggtta ctggtctaac acttaaagag gcaggacagt tagctatccg gttcgtagag
     2941 tcaatgagga aacctattgg tgaaaaggtt gaagggtcat ttatcggaga actctcagct
     3001 ttgaccagtg ttaggaattt ccctgtaagg gtgaaatgcg ccttattggc atggtctgct
     3061 cttgaggatg ctatttcaga aagaaaataa atcaacgatc aagccacact ctttcccaat
     3121 gtgaatactc caagccagaa aagtttggat gcatgttttt taaatcctca gtccatgccc
     3181 ataggctttt ttgggttgca ggcatgaagc gatatgattt ctccagtaga tgtctgttca
     3241 tttcattgat gatctcagag cgtttttcaa ggttgtattc ttgggattgc tcttctataa
     3301 gttcatctag cctcatatcc cggtgatttg aggtattcca ttttccttca ctatgaagta
     3361 cagggataac aaagctgttg gtcgatgatt gaggaaatgt tggtcccatt aatacatcga
     3421 aatcaccctc cccccagatt ttttcagcat attgcctgcg gttcacaaaa ttaattattg
     3481 gttcaaaccc cacagcttca agctgtttgg aggcaatttc cgcgctctcc ttatactttg
     3541 atccgaaatc gcttgtggct atcgatatcg ggataatttc ttttaatgtt tctgggtgtt
     3601 gcgaagggta aaaatatgat tcccaatcag accttacggg aatccacgaa gagtgattgg
     3661 tgacaaaccc taaagagaag aaagcatagt tatgccaggc tttttggata atctcgttag
     3721 ggtttatcga tgatctggct aaaacccttg tgtgagcatc cttaaatggt ggtttagccg
     3781 tgttaaaggc tatttcaaaa ccgagtcctg gttctggatt ttcaagtgct atgtctagct
     3841 ctgttgaata attctcgatg ttataaacgt caatcaatcc cacactatat gcgcttaatc
     3901 gtgcttgagg atctgtgatg atatgaaact ctatagaatc caaatatgga gggttaggtg
     3961 ctgaaaaaac attttctact gaggaataag aatcatctac aagcttcgac aatctccaag
     4021 gaccggaacc aataggaggg gctgattcca cttttcctgg gtcgttgatg tctgattttg
     4081 ataataattt agatcttccg tctgccaaag atataaaaaa atctgcatcc gggacattta
     4141 aatttatctg aatctgattt tcagacttta cgtctacgct gcggaccata tgaaatctgt
     4201 tcgaatgttc ctgattctta gccatttggc gatctaagct aaattttatg tcttctggca
     4261 acaattgatt catatcgtcc gcctcttcag acacccactt gatatttgta tcgacatcaa
     4321 aaataaaagt tttgttgtct accatctgcc atgaactgca agcatcgcat tcagtggatt
     4381 tagaaggtaa tgtgatatcg ggtccattac taaatttcaa gagtctttga taaattattc
     4441 ctattcccca agtggagtgt gctccagaat aattttcatg agggtctaat gagtcaaaat
     4501 aatgtttcga aattaatctt aaagttccgc ctcgtttgcc ttcaaatatc ggcgggattt
     4561 tatttatagg aatggccggt tccaatgaat tatctggaga gccattttct acggataaag
     4621 gggtaggggt caccatgttt tggataggag agggtgttgg cttaggactt aggtctatcc
     4681 ctattgtagg taggggagta ataactgcat cgacagaagg ttgattctcg aaaaattcct
     4741 gttccaattt ggattgatcg gcacatgcaa aagaaacaaa gcctgctatg aagccaaacg
     4801 ttactgccag gtgtaccaaa tgtttcatat tcaatgatta aactttatgc tgattgatga
     4861 tttaagtata atcaacccca gtaaccaatg acattacttt ttgatggtcc catatgttga
     4921 ttgacaccca tgttcattta gatgcttttc cagattgtga tattgaagct atcttagacc
     4981 gaggccatga tgtgggagtc ggatttgtca tatgcgcagg tacgacggtg gattcctcta
     5041 tccgatccgt tgatctctca cgaaggttcc accggttttt ttctggggtg ggagtacatc
     5101 caatggacat atccaagccg ttgactacag gagactatca aacattagct gaattagctt
     5161 caaatgaacc aaaggttata gtgatgagcg aaatcggatt agattatatg gaaggtatgc
     5221 ccgacagggc gtggcaattt acagctttca gagagcaaat cagtatagcc agggcacaga
     5281 atttacctat cgtttttcat agccgagagg ctcatcagga ctgtcttaga gttttgaaag
     5341 aagaaaaagg ctatgaagtg ggtggaataa tgcattattt tcaaggctca ctagaggagg
     5401 ctcatcaggt aatagatctg ggcttttata tatcgattgc taggccaata tttcggttgc
     5461 cgcatcttca agaagtagtg gctaaaatcc ctatagagaa catagttatt gagacggatt
     5521 cggcgcctca gccgtttaaa aagaatcggg ataattggac agaacctcgt tatctaaggc
     5581 ctatcacgga aaaaattgct gagctcaaag atatggatat agacatagtg gaagcatcac
     5641 tgtttcaaaa taccaaaaaa ttgctatcaa gccagtggga aatcgtcacc caatccgtgc
     5701 ctaattccta atccagcata ggattcgtga aatatgggcc gataatcatc aaccaaggaa
     5761 gtaatatcca tgccatgaac cagcctattg ccgaagggaa aaatgcttta aggagggtaa
     5821 tgttatgagt cgtttttact ccgatggtca ccgcgactaa cagccacact aaagttatca
     5881 atagtatgta atcgcccact atagggatcg ttgctagaat taatccaact ccagggccat
     5941 atgctatgcc agtactacgg atagtgtctc taaactctgc atctcccccc ataagaccgc
     6001 ataatgtgtt gttgatgaat gaccacatca tcatcccaac aaaaattgtg gaaaatgcca
     6061 cagccatcat ctgaagacga gcaaaatcac tctctggatt aatcacggta cctttgtaat
     6121 gcaggtatat tgcgcttgaa aaggccgcga tcgccaccgt tcccaaagat tgtatgacga
     6181 gctctggcct gcttttaaat tcagggtaaa tatttttatt taagatggcg gctttaacga
     6241 ggttatctat cataaatggt atttgttata gtactgccgt ctcaggttcc gacaaaatag
     6301 taataatagt gtaaaattct taagttctaa atcctagcat aactggtgta atcctcatcg
     6361 actcatcgat cctaacacaa ggtgcctatt taacagttat tggtatgggc acatcatttc
     6421 tactactttt aatattgatt acatcaatat tagtgactaa atacgtttcc aataaaatag
     6481 atcctcctta cgtgcctcct actgaagaac agaaacaaga gttggcgaga aaggcaaagg
     6541 cggcggcggc ggcctttgcc tttctgtccg ctaaaggacg tgatcctaaa atagattcgg
     6601 aagtgaaata atgaaaccag tcccatcgat ggtgtatatg aatgatagaa caacttcttg
     6661 attttataag aacaacaggc ctcttcaata tggggtggcg tgatgcggtg atgatttttg
     6721 ttggtcttat ttttatatat ctcgccataa aaaaagactg ggaaccatat gaacttttac
     6781 cgataggatt ggggataata gcagcaaatc tacctctgac tggtttaatc acaccaccaa
     6841 cttcagattc tttgaatcaa gaggcgggaa tattcggtat cttttttcac tacggtttat
     6901 ctttttggaa tatattgcct cctattattt ttctaggaat aggggctttg acagattttg
     6961 gcccggtgat agcaaatcct aaaaccttac ttttgggcgc tgccgctcaa ataggaatat
     7021 ttgtcgcatt ctggggagct ttaatttcag gttctttagg aatgaatttt ggaattgagg
     7081 aggctgcctc aattggtatc atcggtggag cagacggccc tacaacgata tttctttcag
     7141 cacggttggc accggaaata ttaggtataa cggctgttat agcctattct tatatggcag
     7201 cggttgcatt tatacaaccc ccattaatga aactgttcac taccaaaaaa gagcggcaaa
     7261 tagttatgcg acctctcaga gaggtttcaa agcttgagaa attaatattt cctaatgtgg
     7321 ctctaatagc gataatattg gtggttccta aatctgcccc attgattgcc atgttcatga
     7381 taggtaattt gtttcgagag agtggagctg tccctcgatt gacaaaatct gcatcaaatg
     7441 agattttgaa tattgccacg attttcctaa tgattacggt gggtacccaa ctcaccgctg
     7501 acagagtttt cgattggcag actatggtga tattgattct tgggttggta gctttttctt
     7561 gtgggactat cggagggata ctttttgcaa aaatcatgaa cttatttctc aatgaaaaga
     7621 taaatcctct cattggttcg gctggtgttt cagctgtacc aatggctgca cgtatttcac
     7681 atcacctggg acaacaggcg aatccgaata attttttgtt gccccatgcg atgggcccta
     7741 acgtggcagg ggtgatagga tctgctgtta tagcaggtgt ttttatatca ttggtggcct
     7801 agtcttatga aaattcgagt cgaagaaaaa tggtataaca tcgaggtaga cagcatctcc
     7861 tctgactccg cagttgtttt agttgatggt gaacgatttg aggtgaaatt agaggatgtg
     7921 gagaaaacag ctgaactaac cgaggaaatt tccggtcctg tttccaactc tgttggaatg
     7981 agaacggaca attacaagga attcaaaagc ccgatgccag ggaccataat atctattttg
     8041 gtacagactg gggatgatgt gtctcaaggt caggatatat gtgttttgga atcgatgaag
     8101 atgcaacaaa ctcttaaatc agatatggcg ggcactatta tggggacaag tgtgtctcaa
     8161 ggtgatcaaa tattggacgg agacgtaatt ttccttatta ggtaaatgcc attagtcgat
     8221 cccaattagt gctctagatt tgctaaggat gattctgcaa taagcaattc ttctgtttct
     8281 ctgatgggat gaattaaatg aatagccatt tgcaactcct tttttgcctc cactaagttt
     8341 ccattctttt tatgtattaa acctaggtac agatgagtcc ctccaaggtc ttcatccaaa
     8401 gtcaacgctt tttctaggtc tgttttagca agttcgtatc tctccaaatg gtaataagct
     8461 gcacctctag atttgtacgc aatggctagg tcaggcattc ttttaattag ggtggaaaag
     8521 ctgttaacag attcgatgaa ctgctccgat ctcaataaat taaggccgcg attatagaga
     8581 aacagtgctt ctatttcagg ggttggtgat gccgatatag ggtttgttgg tgtgatagta
     8641 ggggtgattt cggtttctct ggaaatttta tttgctgtgg gcaccggttc ttgggtaggg
     8701 acgagtaaag tagccccatt ggagttatta tcacaagata gtaatgagca aactataatt
     8761 gtcacagcga tcaatggcag gtgcctgaaa tatgaaatca agtttacgtt gtattgcatt
     8821 ggaaataaaa aaaattagaa taggaggaag ttagttgatt cttattctat catctcaatg
     8881 aaattggagg atatgttgat aaaaaccctt gaagataaaa ccccactaat tcacccaact
     8941 gcttttgtca gtgaagcggc ttatatagta ggtgatgttg aaataggtga aggatcaagt
     9001 atttggcccg gaaccgttat aagaggcgat agaggtaagg taaaaatcgg caaatataca
     9061 aatattcaag ataattctgt ggtgcatgga gacgctgacg tcgaaatcgg tgactacgta
     9121 actgtaggcc atagggtagt gtgtcattct caatacatag ggaatcagtc actgatcggc
     9181 aatggggcta tattaaacga cggggtgata atcggggaaa atagtgtggt tgggtcgggg
     9241 gctatgctgc tagagaacat ggatatccca aagaggtcca ttgttgtggg tatgcccggg
     9301 agagttagag gtgagattaa caaaaagcat ttagatttgc aggtggaact tgccgatatt
     9361 tattccgatt tagccaagcg atacaaaaac gaaggcgctc ttgaataaaa ggactgtttc
     9421 agggaagaat tacgggagtt ccatgttccg ggtgagtgat tattgtcact tctgatccat
     9481 aaatggattt aatgttattt tttgttaaca cttctttagg tgttccgtct gcagcgatag
     9541 atcccttcga aaatagaagg attctatcac tgaattgagc cgcaatattt agatcgtgta
     9601 tagccagaac agtgacccca aactcagatt tttgctcgtg tatctttttt atgattttca
     9661 taactttcat ttgatgagaa atatctaaat tggaagtcgg ttcatctagt agcattattg
     9721 gtgattgttg ggtaatcgcc agagctaaca tagcacgttg tttttccccc cctgaaatct
     9781 cccctaattt tcttttggag agataatcta tttccgtcaa aaatatggat tcgcgtgcta
     9841 tattaaaatc ttcttttgat tcccattgaa gtaatccaag gtgaggatta cgtcccaaca
     9901 tcacgaaatc tgaaatgcta atttcgtctg gaagtttggg attttgcggg accattgata
     9961 cccaaatagc tcggttctta ggtgtgagat cgtaacttag ctgatcatat atatatattt
    10021 cgccagatga gggttttaat gtgccggata gtagtttcaa tagggtagtc tttccagctc
    10081 cattcggccc tactagagaa actaattctc ctggcttgaa acctgttgtg atttcttgaa
    10141 tcacattcat atttccatat ttgaatgaaa cattatgaag attaataacc atcaatagtg
    10201 ctccctttag ttcttaaaag cacataaaca aaaaaaggag accctactaa cgcagttatt
    10261 attccaactg gcaattcaat gggactcacg attgttctag ctgtcaaatc ggcgagaatt
    10321 agaaaagcag cacctgttag gccagaaaga ggtagtaatg ttcggtaatc agtgccccat
    10381 aaaagccgta tgatgtgagg ggctattaac cctacaaatc ctatcagtcc actaaatgag
    10441 actgcggcgg ctgtgaccaa ggttgctgat actatcaata tacgtttgct cttctctatg
    10501 ttgacaccta acgattcagc gtaatatccc tcaagttgca tggtgtttaa tatgcggctg
    10561 tggctcaaaa tgaaactcac gcctactatc atgtagggca ttattaatat tgagtgagac
    10621 cattgggccc ttatgaatcc ccccatgagc cagctgagta atggcctcaa atcaggatca
    10681 cttcttaaca tcagaatccc agtgatagct gtcgcaaatg ctccaatggc caatcctgct
    10741 aaaagtaatg tcgacaagtg ggtgttgcca gactttatgg agattaggta tgcgataaaa
    10801 acggctgtaa ttcctccaca aaacgaagct actggcagga gactgaagta tccatacgtg
    10861 aagggtattc ctgagaggaa aacaatggta gcgccaagtg atgctcctga ggcaaccccg
    10921 attaaatatg gatccgccaa ggggttttta aataaaccct ggtatgtggc ccctgaaatg
    10981 ccaagggatg ctcccacgag taatgcaagg acaactctgg ggaatctaat gttccatatt
    11041 attgtgtgcc aagcagatgt cagttcccct gactcgttaa acaaatctaa tttagaaaat
    11101 aatattaaaa atacagaagt tatgggtata tgcactgacc caacagccac accactaaca
    11161 gcacaaatga gtattacaac tatcccgaga gttattttat tccaaggata tttatacgct
    11221 aatacaaggt cttgattact ggtattagct ggtgatttca tttgaaaagc ccaggataaa
    11281 attcatttgc tagctctt
//
LOCUS       DASCNS010000130         8818 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001088393, whole genome shotgun sequence.
ACCESSION   DASCNS010000130 DASCNS010000000
VERSION     DASCNS010000130.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001088393"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(65..862)
                     /gene="trpC"
                     /locus_tag="QF838_04580"
     CDS             complement(65..862)
                     /gene="trpC"
                     /locus_tag="QF838_04580"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HJO59996.1"
                     /translation="MINPDTPNILKEIVQIKQERLKARKRDMPRQEVESLISSRERPL
                     NFSGCLMGDSVRVIAEIKRGSPSKGVFSKGIKASELAAVYAENGAAAVSVLTNEDHFY
                     GSIEDLLEVHNKVYQSGVPVLRKEFIFDPYQIYEARAYGADAILLIVSMLDKDQLIDF
                     TNIATSLWMQCLVEVHDEKELELAVESGAEIIGINNRDLRTFNTTLETTEILAPKVPL
                     GKISVSESGISDKSDIERVMKAGVGAVLVGESLVTADDPAIALRNLL"
     gene            complement(868..1881)
                     /gene="trpD"
                     /locus_tag="QF838_04585"
     CDS             complement(868..1881)
                     /gene="trpD"
                     /locus_tag="QF838_04585"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HJO59997.1"
                     /translation="MITDLIEKLVQKENLTFDEAASAMTLIMEGEATPSQFGSLVTAL
                     RMKGETVDEVSGMASVMRSMSLHVETESDLVDTCGTGGDGFGTFNVSTAAAFVAVGAG
                     VHVAKHGNRAMSSKSGSADVLEALGVNIQLTPQQIATCLEKTGFGFMFAQSFHPSMKY
                     AALPRREMGIRTVFNILGPLTNPAGATRQVIGVADPLVGELMANVLKKLDSERALIVH
                     GDDGLDEITVSTTSTVWELKAKGVKTYQINPEDYRIELSPLDQIKADSVEESASIIGD
                     VLLGKTGPARDIVLLNAAASIFVAKEGSSNFGHCVSLSKDSIDSLEARKALDEHVKLS
                     NLI"
     gene            complement(1903..2466)
                     /locus_tag="QF838_04590"
     CDS             complement(1903..2466)
                     /locus_tag="QF838_04590"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HJO59998.1"
                     /translation="MILLIDNYDSFTYNLYQYLAELGAEVFVVRNDKITLQEITKMGP
                     EKIVISPGPCTPDKAGISNDVIKHFGPTVPTLGVCLGHQCVGHSYGGVVGSAGEIMHG
                     KTSAVHHDGKGVFNGLPNPFNAVRYHSLAIFKEDLPDDLEVTAWTESGLIMGVRHKNH
                     PVQGIQFHPESIMTENGKELLENFLKY"
     gene            complement(2467..3918)
                     /gene="trpE"
                     /locus_tag="QF838_04595"
     CDS             complement(2467..3918)
                     /gene="trpE"
                     /locus_tag="QF838_04595"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HJO59999.1"
                     /translation="MYYPRLPEVKRMSSQGNLAPIYREISADLETPVSAYLKVARAPY
                     SFLLESVEGGEHLARYSFIGTEPEDVIETGPGWKDGEVDPLRLVESILSKYKVVNSGH
                     LPRFMGGAVGYLSYEVARYFEDIPHDNKNVLDVPESVFMLTKTFLVFDHVRHVIQVVS
                     HVKLDGVVEDEYVKATERIDGIIERLNSPLDLSDIPSESGFGSGDKISNMTPDYHQSM
                     VEDSQKYVVAGDVIQVVVSQRFSRPSSAHPFHIYRSLRAINPSPYMYYLNLNGFQIVG
                     ASPEMLVQVEGGVVSTHPIAGTRPRADNPEEDRRLEQELLSDEKERAEHLMLLDLGRN
                     DIGRVSEPGTVEAVEIMGIERYSHVMHLVSHVEGKLKAGYSNYDALRACFPAGTVSGA
                     PKIRAMEIIAELEEDSRGPYAGAVGYFDFVGNMDTAITIRTLVFKDGVAHFQAGGGIV
                     YDSVPETEFYETIHKAGALMRAIEDAENKLDAH"
     gene            complement(3961..4956)
                     /gene="trpS"
                     /locus_tag="QF838_04600"
     CDS             complement(3961..4956)
                     /gene="trpS"
                     /locus_tag="QF838_04600"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HJO60000.1"
                     /translation="MKRVFSGVQPSGDPQLGNYLGAFKGWVDRQSEKENFFCVVDLHA
                     LTVNPDPVQLVSQTRDLAAILFACGLDPQKSTLFVQSHVPAHAEGCWLLNCVTPLGWL
                     ERMTQFKDKSEGRGSVSTALLDYPVLMAGDIIFYDAHEVPVGEDQKQHVELARDIANR
                     FNSIYGETFVVPEPVIPDIGGRVMGLNDPMVKMSKSYSHIRGHAVRMLDDPKEIERSF
                     MRAVTDSGNEILFSEEEEKAGVNNLLGIYKVITGKNEKEVERDFESARGYGDLKKAVA
                     EVVIEELTPIREQYEHLMNDVSELDRLLAIGAEHAASISTPKVREMKEKMGLIVP"
     misc_binding    complement(5018..5254)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5282..6412)
                     /locus_tag="QF838_04605"
     CDS             complement(5282..6412)
                     /locus_tag="QF838_04605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60001.1"
                     /translation="MKFGLLYEMQRPHSDFQVDYSSLIDETIEQCRLADEVGFDHLWL
                     VEHHFLTTFSGSSAPEVIISALSKLTKKIRIGFGVVVLPNHHPIQVSERISMVDHLSG
                     GRVEFGVGRSSPYEQLGLGIDPRDTREIMEESLNIIPKIWQTEGLFSHKGKHFDIPER
                     EILPKPKQSPHPPIWMACTQATSYEMAAEKGIGVLSFGSGAPAGMKEHVENYRENIKN
                     ANPVGGFVNNQWANFTLGHCGYDDQEAKEIGASAIKEFFGPNRPYTADRADVYEKLLD
                     SWGGIPDHLQANFSRFLGGEEDLGGGGAPRAMLGEMSAELLAERGVIVAGNPESCIES
                     VRRHEEIGVDQLLLIMQSDQVPHEKVMTSIELFGKEVIPAFQ"
     gene            6538..7458
                     /locus_tag="QF838_04610"
     CDS             6538..7458
                     /locus_tag="QF838_04610"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HJO60002.1"
                     /translation="MNILIMAAGAVGGYFGSVLSYSNDVTFVARGEHLRKIQEYGLSV
                     KSVTSGNYVCWSKAYKEPPPEYKADLAIFCVKEYQNEQACEIIANSISDNTTILTLQN
                     GLGSGEFLGQKFGSEKVVLGAAYVEASKGGPGEIFEHGGGCSITFGKQSDIQEGRVRE
                     LANIFESSNVDFTVSENIHDSLWQKLVFISALSGMTCITKSTFKEVMNHPETRNITIN
                     LVRETASVASKIVPNFSKDTVQNTIDHLETHKNDLVSSMHQDLLAGKPMEINAINGAV
                     SRKGKTLGIATPINDMIVSCLSLQDTRARK"
     gene            7484..7813
                     /locus_tag="QF838_04615"
     CDS             7484..7813
                     /locus_tag="QF838_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJO60003.1"
                     /translation="MKYFYRAEDANQRELAPGVKLKTMWGDKIMMSLVELDPGSEVPL
                     HSHPNEQAGMVLEGEFEFTIGTESKKVKEGEYYIIPGGIEHRVVAGPKPARALDIFSP
                     PREDYKS"
     gene            complement(7867..8325)
                     /gene="sodN"
                     /locus_tag="QF838_04620"
     CDS             complement(7867..8325)
                     /gene="sodN"
                     /locus_tag="QF838_04620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HJO60004.1"
                     /translation="MKIISRLFGDVQAHCDVPCGIYDPISAKIAAQTVLKMAIRLEAV
                     DFSSGAPDPGMPNSVARYVAVKEEHAQGVKNELNILWSDYFKPEHLSDYPNLHELFWN
                     ADKLAGANKQGVSSSSAKELVDAVDGIAKIFWATKGVDYSDPNADVRFGA"
     gene            8506..>8818
                     /gene="lepB"
                     /locus_tag="QF838_04625"
     CDS             8506..>8818
                     /gene="lepB"
                     /locus_tag="QF838_04625"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HJO60005.1"
                     /translation="MEPTLANNVLILTKTFRFPYRSQIVTFKDPINFTDTIIKRVIAI
                     KTDHLKITEGSIYLNEESLKEPYIEGLPKTMSIEEEFNWNLENDSVIVLSDNRLSGNL
                     DS"
BASE COUNT         2545 a         2117 c         1624 g         2532 t
ORIGIN      
        1 gccccatttt ctcgggcaac aagggcattt cgtgagtcct ttaacccaca tattttaaac
       61 aaagtcatag taaattccta agtgcaattg caggatcatc tgctgttacc aaagattctc
      121 ccaccaaaac cgcgcccact cctgctttca taactctttc aatgtcagac ttatctgata
      181 taccactttc actaactgaa atttttccga gcggtacttt tggagccaaa atttcggtcg
      241 tttccagtgt ggtattgaac gttcttaaat ctctattgtt gatcccgatg atttcagctc
      301 cggattctac agccaattca agttcttttt catcatgcac ctcaaccaaa cattgcatcc
      361 atagtgaggt tgcgatgttt gtgaaatcta tcaattggtc tttatccaac atagatacaa
      421 tgagtaaaat tgcgtcagcc ccataagccc gagcttcata gatttgataa gggtcaaata
      481 tgaactcttt ccttaaaaca ggcacacccg actgataaac tttattgtga acttcaagca
      541 aatcttctat gctgccataa aaatgatctt cgtttgtaag aacggaaact gctgccgcac
      601 cattctcggc gtacactgcg gccagttcag aagctttgat accttttgaa aagacacctt
      661 ttgaaggcga tcctctcttt atctctgcaa tgaccctaac ggaatctccc atcagacaac
      721 cagaaaaatt taacggccgt tcacgacttg atatcaaact ctctacttct tgcctaggca
      781 tatccctttt acgtgctttc aatctctctt gcttaatttg gacaatttct ttcaaaatat
      841 taggtgtgtc aggattgatc attaaactta aatcaaatta cttaatttta cgtgttcatc
      901 aagggccttt cttgcttcaa gagaatctat cgagtcttta ctaaggctta cacaatgacc
      961 aaaattggat gacccttcct tcgcaacaaa aatcgaggct gcggcattaa gtagaacaat
     1021 gtctcgtgca gggccagttt tccccaacaa tacgtcgcca atgatacttg cgctctcctc
     1081 gacactatca gccttaattt gatcaagggg tgagagctct atccggtaat cttcagggtt
     1141 tatctgataa gtcttaacac ctttagcttt aagttcccac acggtactag tagtagatac
     1201 cgttatttca tctaagccat cgtccccgtg cacaattaat gctctttcac tatctagttt
     1261 ctttagaaca ttcgccataa gttcccccac taaaggatcg gcgactccaa taacttgtcg
     1321 agttgcgcct gcgggatttg taagaggccc taaaatatta aagactgttc ggatacccat
     1381 ctccctccga ggtagagcgg catatttcat cgagggatga aaagattgag caaacataaa
     1441 gccaaatcca gtcttctcta aacaagttgc gatttgttgg ggtgtcaatt gaatattaac
     1501 tccaagcgcc tccaaaacat ctgcgcttcc gcttttacta gacatagcgc gattcccatg
     1561 cttggcaaca tgaacaccag ctccgacggc gacaaaagca gcagctgtag acacgttgaa
     1621 tgtcccaaat ccatcacctc cggtaccaca tgtatcaact aaatcactct cagtttccac
     1681 atgcagagac atagagcgca ttacagaggc catacctgat acttcatcta cggtttcccc
     1741 cttcatccga agggcagtga ccaatgagcc gaactgtgaa ggggttgcct ctccttccat
     1801 tattagggtc attgcagaag cggcttcatc gaaggtaagg ttctcctttt gaaccagttt
     1861 ctctatcaaa tcagtgatca taaattaatc ctttgaaagt cattaatact tcaaaaaatt
     1921 ttccaaaagt tccttcccat tttcagtcat tatagattca ggatgaaact gtatcccttg
     1981 cacaggatga tttttatgcc taacgcccat tattaaccca ctttcggtcc aagcggtaac
     2041 ttccaaatca tcaggaagat cttccttaaa aattgcaagc gaatggtaac gaaccgcatt
     2101 gaatggattc ggtaatccgt taaacacccc tttaccgtcg tgatgtacag cagaggtctt
     2161 gccgtgcatt atttctcctg cactaccaac caccccacca taagaatgcc ccacgcattg
     2221 atgacctagg cacaccccta gtgttggtac ggttggcccg aaatgtttta taacatcgtt
     2281 tgaaataccg gccttgtcag gagtgcatgg gcctggagat ataactattt tttcaggccc
     2341 cattttagtt atctcttgca acgtgatttt atcgtttcgg acaacaaaaa cttcagctcc
     2401 caattcagcc agatactgat acaaattgta agtaaagctg tcgtaattat ctatcagcag
     2461 aatcatttag tgagcatcca atttattttc agcatcttct atggctctca ttagggctcc
     2521 agccttatga atcgtttcat aaaattctgt ctctggtacg ctatcataga ctatccctcc
     2581 tcctgcctga aaatgcgcca ccccatcttt gaagactagc gtccttattg tgatggcagt
     2641 gtccatattt ccaacaaaat caaaataacc aacagctccg gcataggggc ctcttgaatc
     2701 ctcctccaat tctgcaatta tctccatagc ccttattttg ggggcaccgg atacagtccc
     2761 tgcggggaaa cacgctctaa gagcatcgta atttgaatat cctgctttta gtttcccttc
     2821 cacgtgggac actaagtgca taacatggga gtatctttct attcccatta tttctacagc
     2881 ctcaacagtt ccaggttcgc tgactcgtcc gatatcattt ctaccaagat ctaataacat
     2941 gagatgttca gctctctctt tttcgtcaga tagcaattct tgttccaacc tacggtcttc
     3001 ctcgggatta tccgccctag gccgggtacc cgctatcgga tgcgtagaaa ccacaccacc
     3061 ttctacttgg accaacattt ctggtgaagc tccaactatt tgaaatccat tcaaattaag
     3121 atagtacata tatggagatg ggtttatagc tctgagggag cgataaatat gaaatggatg
     3181 agctgaagaa ggccttgaaa accgttgaga aaccacaact tgaattacat ctccagccac
     3241 cacatatttc tggctatcct caaccatgga ttgatgatag tcaggtgtca tattggaaat
     3301 tttatctcca gatccaaacc cggattcaga gggaatatca gagagatcaa gcggtgaatt
     3361 aagtctctct ataattccat caatgcgctc agtagccttc acgtattcat cttcaaccac
     3421 accatccaat ttaacgtgac tcaccacttg aatcacatgt cgaacatggt caaaaaccag
     3481 gaaggttttt gtcaacataa aaactgattc cggaacatca agaacattct tattgtcatg
     3541 ggggatgtcc tcaaaatatc gagcaacctc ataggagaga taaccaaccg ctccccccat
     3601 aaaccgagga aggtgtccag aattaaccac cttatatttc gatagaatag attccaccag
     3661 tcgaagagga tctacctctc cgtctttcca tcccggtccg gtctcaatca catcctctgg
     3721 ttcagttcca ataaaactat atcgagctaa atgttctccc ccttccacac tttctagcaa
     3781 aaaagagtag ggagcacgag caaccttaag gtacgctgag acaggagtct caagatctgc
     3841 actaatctcc ctgtaaatag gagcaagatt accctgagag ctcatccttt tcacctcagg
     3901 aagcctagga taatacatgt tggcactcca tgaattttga agtcagatgg attatcttaa
     3961 tcacggtacg attaacccca ttttttcttt catttcacga actttgggtg ttgaaattga
     4021 ggctgcatgc tcagcaccaa tagccaacaa tctatccaat tcagaaacat cattcatcaa
     4081 atgttcatat tgttcacgta tgggagtaag ttcttctata acaacctctg ctactgcttt
     4141 ttttaggtcc ccgtatcctc tggctgactc aaagtctcgc tcaacctctt tttcgttctt
     4201 ccctgtaatg actttatata tgcctagcaa gttattgacc cccgccttct cttcttcttc
     4261 agaaaacaga atttcgtttc ctgaatcagt aacggcacgc ataaaagatc tttctatttc
     4321 ctttgggtca tctaacattc gaacagcgtg acctcttatg tgagaataac ttttggacat
     4381 tttcaccata gggtcgttaa gacccataac acgaccacca atatccggta ttacaggttc
     4441 aggcacaaca aacgtttccc cataaatgct gttaaatctg ttagcgatat ctcttgcaag
     4501 ttcaacatgc tgcttctgat cctccccaac aggaacctcg tgggcatcat aaaaaattat
     4561 gtcaccagcc atcaataccg ggtaatccaa taaggctgtc gatacactgc ctctaccttc
     4621 tgatttgtcc ttaaattgtg tcatcctttc taaccagccg agaggtgtca cacaatttaa
     4681 aagccaacat ccttcagcat gggctggaac atggctttgg acgaacagtg tgctcttttg
     4741 aggatccagc ccacacgcaa acaaaattgc tgcaagatct ctagtttgag aaacaagttg
     4801 cacaggatcc gggtttactg ttaaggcatg gaggtcaacc acacaaaaaa aattctcctt
     4861 ttctgactgt cggtctaccc aacccttaaa tgcgcccagg taattcccca attgaggatc
     4921 cccacttggt tgtaccccac taaacactct ttttatttct tccatttctt cctgcaatta
     4981 tttttcctgc aattatttca aaaaaaaacc gcccctcgaa aggggcggtt atattaccgc
     5041 ggtgccaccc ttattcctct tagtaactaa gaggcactct atggaattta attccaggct
     5101 cttttaacgg ggcctatccg tctgttatcc aaccttacag gttagttaac agcagctccc
     5161 aggtccattc actacctgcg cttacaccgg ctcacagctt acccggctct cttagtatcg
     5221 ctagatggtt actattcctg ttcttcgcct ttttaagtaa ggtaattata ccgcataatg
     5281 tctactggaa cgccggtatt acctctttcc caaacagttc aatagaggtc ataacttttt
     5341 catgaggcac ttggtctgac tgcataatta aaagaagttg atctacaccg atttcctcat
     5401 gccgacggac agattctatg catgattcag gattgccagc gactattaca cctcgttcag
     5461 ccaaaagttc tgccgacatt tctcctaaca tggctcttgg ggctcctcct ccccctagat
     5521 cttcttcacc tcccaagaat cttgaaaaat tcgcctgtag atgatcaggg attcctcccc
     5581 atgagtctaa caatttttca tatacgtctg ccctatcagc cgtatatggc ctgttaggcc
     5641 cgaaaaattc cttgatagca cttgctccaa tctcttttgc ttcttgatca tcatatccac
     5701 aatgccccaa tgtgaaatta gcccattgat tgtttacaaa acccccaacg gggtttgcat
     5761 tttttatatt ctccctgtaa ttttcaacat gttccttcat acctgcaggt gcaccagacc
     5821 caaatgacaa aacacctatt cctttttccg ccgccatttc ataactagta gcctgcgtgc
     5881 atgccatcca tattggaggg tgaggagact gtttaggttt gggtaatatt tccctttctg
     5941 ggatgtcaaa atgtttgccc ttatgagaaa acaacccttc agtttgccaa attttaggga
     6001 tgatattcaa agattcttcc atgatttctc gagtatctct aggatcgatt cctaacccta
     6061 gttgctcata tgggctgctg cgcccaacac caaactccac tctgccacca gagagatggt
     6121 ctaccataga tattcgctct gaaacttgta ttggatgatg attaggcaga acaacaacac
     6181 caaaccctat cctaattttt ttggtcaact ttgatagggc agaaattatc acttcgggtg
     6241 cagaggagcc agaaaaggtt gttaaaaaat gatgttctac caaccataaa tggtcaaacc
     6301 ccacctcatc agccaaccga cactgttcga tagtttcatc gattaaagat gaataatcaa
     6361 cctgaaagtc gctatgggga cgctgcattt catacagaag tccgaatttc atattctatc
     6421 tcctacggaa gtgatcttat tttggtgcag tctaacacaa gctaaattgc tcctcgataa
     6481 agactaatta tgtgtacact atcaacacga ttctcgccat acatggtagg aggtagcatg
     6541 aatatattaa taatggctgc tggagctgta ggtggttact ttggatccgt tttatcctat
     6601 tctaatgacg tcacttttgt ggctcgagga gaacatttac gaaaaatcca agaatatggt
     6661 ctttcagtaa aaagtgtcac cagcggaaat tatgtgtgct ggtccaaagc ttacaaggag
     6721 ccccccccag aatacaaagc tgatctggct atcttctgcg taaaggaata ccaaaatgaa
     6781 caagcctgtg aaatcattgc caattcaatt tctgataaca ctaccatatt gactctgcag
     6841 aacggcttag ggagcggaga attcctaggc caaaaatttg gaagtgaaaa agtcgtacta
     6901 ggggccgcat atgtcgaagc ttccaaaggc ggacccggag agatcttcga acacggagga
     6961 ggatgtagca taacttttgg caaacaatct gacatacaag aaggccgggt aagagaactt
     7021 gcaaatatct ttgaaagttc gaatgttgat ttcacagtgt cagaaaatat acatgattcc
     7081 ctttggcaaa aattggtttt tattagcgcc ttaagtggta tgacgtgcat aaccaaatcc
     7141 acttttaaag aagtaatgaa ccatcctgaa acacgaaata tcactataaa tcttgtgcgg
     7201 gaaacggctt cagtggcatc taaaatagta ccgaattttt ctaaggatac tgtccaaaat
     7261 acgattgatc atctggaaac tcacaaaaat gatctagtct catcgatgca tcaagatcta
     7321 ttagccggca agcctatgga aattaacgct ataaatggtg cggtcagccg gaaaggaaaa
     7381 acccttggta ttgccacccc gatcaacgac atgatagttt cttgtctttc actgcaagac
     7441 actagggcta gaaaataatc actcactcag gaaaggaatt tttatgaagt atttttatag
     7501 ggccgaggat gctaatcagc gagaacttgc acctggggtg aaattaaaaa ccatgtgggg
     7561 ggataaaata atgatgagtc ttgtcgaact tgaccctggt tctgaagttc ctttgcacag
     7621 ccatcccaac gaacaagccg gtatggtact tgaaggagag ttcgaattca ctattggaac
     7681 tgagtccaaa aaggtgaaag aaggcgagta ttacattatc ccaggtggca tagaacacag
     7741 ggttgtggcg ggccccaaac ctgcacgggc cttagacata ttcagtcctc ctcgtgagga
     7801 ctacaaatca tagctgtctt acagggtgcc tattcgccaa gacaccctgt atttaaacag
     7861 aattatctat gctccgaacc gaacgtcagc attgggatca ctgtagtcga ctccttttgt
     7921 ggcccaaaaa atcttagcaa ttccatccac tgcatcaact aattccttcg ctgaagacga
     7981 acttacacct tgtttattgg cacccgctag tttatccgcg ttccagaaca gttcgtggag
     8041 atttggataa tcagaaagat gctctggctt gaaatagtct gaccaaagaa tattaagctc
     8101 gtttttaact ccctgagcat gttcttcttt aaccgccacg tatcgtgcta ctgaattagg
     8161 catcccggga tctggagcac cgctagaaaa gtccacggct tcgagtctta tcgccatttt
     8221 cagaaccgtc tgcgcagcta tttttgcact aattgggtca tatataccac acggaacatc
     8281 acagtgggct tgtacatctc caaagagtct tgaaatgatt ttcataaaca accttcctta
     8341 aacctgacct tgcttttcaa ggcccaaaat tttaatttat accagaaacc tattgaccag
     8401 cttctgactg ttaagataca gaaaattact gtccaaggca acaagacatc aaatgaggat
     8461 tgcaaaggct ctatgttcca tattcgaagg gtgaaaaatc gcagtatgga accaacatta
     8521 gccaataatg tgctgatttt gacgaaaact tttagattcc cttatcgaag ccagatagtg
     8581 acgttcaaag atccaataaa tttcacggat acaataataa aaagagtgat agcaataaag
     8641 accgaccatt taaaaattac agaagggtct atctacctaa acgaggaaag tttaaaagag
     8701 ccttatatag agggattacc caaaacaatg agcattgaag aggagtttaa ctggaatcta
     8761 gagaatgatt ccgttattgt cttgtccgat aatcgtctat caggaaattt agacagcc
//
LOCUS       DASCNS010000131         3550 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001095559, whole genome shotgun sequence.
ACCESSION   DASCNS010000131 DASCNS010000000
VERSION     DASCNS010000131.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001095559"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            33..833
                     /locus_tag="QF838_04630"
     CDS             33..833
                     /locus_tag="QF838_04630"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HJO60006.1"
                     /translation="MTTIGIVGCGTIGQALLKASEAGSLTLPITGIASRTEESARAFL
                     ATLNDPPEYCTQPELIAKSDLIVEAAGGDVIPELACATFHSGKDLMAISVGALVAHPE
                     ILDMAKERSCRIYAPSGAIAGLDGIKSASAGRIDSVIMTTRKPLEALEGSPYLLERGI
                     SVLGLTEEQEIFSGTAREACIGFPANVNVSAAVSFAGVGPDRTQIRIIAVPGLDRNCH
                     DIVVEGDFGRLHIEVENIPTENPRTGKLTVMSIIRTLQGITDPLQIGT"
     gene            1050..>3550
                     /locus_tag="QF838_04635"
     CDS             1050..>3550
                     /locus_tag="QF838_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HJO60007.1"
                     /translation="MRIGIDVGGTFTDLVVTRAEGGIAQFKTHSTPVDPSIGVFRGLA
                     EISETYGVTLEQLLLDVELIVHGTTVATNTLVERKGANVALITTSGFRDLLEMREGLK
                     EDRYNLRMASVEPLIPRYLRMGISERVKYDGSVHTPLDVESLRKTLLDMKGEGVESLA
                     VCFLFSFMNPDHELKAEIEIRKVFPDAFVSLSHEVLPQIKEFDRISTTALNAYVGPEL
                     GKYLGNLQQRMVDHGGQRKILIIQSNGGSAPIQDSIKFAVRSILSGPAGGAKGAAKVA
                     SSLGEENVIALDMGGTSTDISIIEGGRPHVANEKFEAGWKISVPMIDIHTLGAGGGSI
                     ARVDIGGALRVGPDSAGAEPGPACYGLGGEGPTVTDAALLLGLLNETSFLGGKASLDK
                     NLSEQAIKSKVADPLGLSITDAAEGILRVVSNSIAEGIRLASVRKGLDPREFSMVAFG
                     GAAGILANQVAREVGINKVLVPQAAPVLSAFGMLTSELRYDFSQSYPAGLNVLDLKGM
                     ISRLRIMEKQGKDQLRENGVTDEEINVEFSADMRYLDQIYEVTVPIPDLDLDGARLKE
                     EWADNFHQRFEELYSYRQSDQEIRLVTLRATVSAPLPEIDDSDSADNSVVDFDQEEAV
                     LRQVYIGSWENVPVLKSDSIPIGQKVPGPAIIESDFTTVVLNQFNEVTKNAEGTLIIG
                     LEDLVSDGSMGGVAAGSDPVTLSVVENRLESIAIEMMDVMLRTSMSQILNSSRDFSTA
                     ILDSRCRLIAQGEGIPVHVSALPIAGEAILNTFGEDIHPGDMFALNDPYNGGSHLPDI
                     TVIKPVFKNGELLFLTINRAHHSDVGGATHGGYNPS"
BASE COUNT          938 a          708 c          950 g          954 t
ORIGIN      
        1 cttaaatgct tgaaggagga tgatctagat cagtgacgac aattggaatc gtaggatgtg
       61 ggacgatagg tcaggcactt ctcaaagctt cagaagccgg gtcccttaca ctcccaataa
      121 cgggcatcgc tagtaggaca gaggaatctg cccgtgcctt tcttgctacc ctcaatgatc
      181 ctcccgaata ctgtacacag cctgaactta tagccaaatc agaccttatt gtcgaggctg
      241 ccggaggtga cgttatacct gaacttgcct gtgcgacttt tcattcgggt aaagacctta
      301 tggctataag cgttggagct ttagtagcac accctgagat attggacatg gctaaggagc
      361 gttcttgcag aatttatgct ccttcaggag cgatagccgg gttggacggt atcaagtcag
      421 catcggcagg aagaatagac agcgtgataa tgaccacgcg aaaacctctg gaggcattag
      481 agggttctcc ttacctgttg gagagaggta tatcagtttt gggattgact gaagaacagg
      541 aaattttctc tggtacagca agagaggctt gcatcggatt tccagccaac gttaatgttt
      601 cagctgctgt tagctttgca ggagttggtc cggataggac acaaattcga ataatagctg
      661 ttcctgggtt ggacagaaac tgtcatgaca tagtagtgga aggtgacttc ggtaggttac
      721 atatagaggt ggaaaatatc cctacggaaa accctcgtac gggcaaacta acagtgatgt
      781 ctattattcg gacactacag ggcatcactg acccgctgca gataggcact tagtgttgta
      841 aatattcggt aacaaattct agggttcgta gaaatctctt cttttctata gcagcatcga
      901 tccctgcgat tattcttctg atgggaatgg tcttatccat caataggctt atgctactat
      961 gctggttcag tggcggtatt catgattgat tttaggtcta tcatgatgga aatgagatgc
     1021 acattcgact atgagggagg aaataagacg tgagaatcgg aatcgacgtt ggggggacat
     1081 ttactgatct ggtggttact agagctgaag gtggaatcgc tcaattcaaa actcactcta
     1141 ctcccgtgga cccctcgata ggagtgtttc gtgggttggc ggagatatct gaaacctatg
     1201 gtgtcaccct agaacagcta ctccttgatg tggaattgat tgttcacggg acgacagttg
     1261 caaccaacac attggtcgag aggaaggggg ctaacgtagc tctgataact acaagtggtt
     1321 tcagggattt attggagatg cgtgaaggct taaaagagga tcgctataac cttcggatgg
     1381 cctctgttga gccattgatt ccccgttact tgagaatggg tatatctgag agggtcaagt
     1441 atgacggcag tgttcacaca cccttggatg ttgaatcctt gagaaaaact cttctggaca
     1501 tgaagggtga aggagtggaa tcactagcgg tatgtttcct gttttcgttt atgaaccctg
     1561 atcacgagct aaaggctgag atagaaatca ggaaggtttt tcccgacgcc tttgtttccc
     1621 tatctcatga ggttctgccg caaatcaaag aatttgaccg gatcagcacg accgcactga
     1681 atgcatacgt tggacctgaa ttaggtaagt atcttgggaa tttacagcaa cgtatggttg
     1741 atcatggggg ccagagaaaa atattaataa ttcagtcgaa tgggggctca gctccaatcc
     1801 aggattctat aaagttcgcc gtcagatcta ttctttcggg acccgctggt ggagctaaag
     1861 gtgcagccaa ggtagcttct tcgttagggg aagaaaatgt catcgccctc gatatggggg
     1921 gtaccagcac tgatatctcg atcatagagg gtggtagacc tcatgttgct aatgaaaaat
     1981 ttgaggctgg ttggaaaata tctgtgccga tgatagacat tcatactctt ggagctggtg
     2041 gtgggagcat cgcacgagta gatattggag gtgccctgcg ggttggccca gatagtgcgg
     2101 gggctgagcc aggcccagcg tgctatgggc ttggaggaga aggtcccact gtcactgatg
     2161 ctgcactatt gcttggtctt ctcaatgaga catcgttcct cggtgggaag gctagcctag
     2221 acaaaaacct atctgaacag gcaataaaat ctaaagtggc cgatccacta ggattgtcga
     2281 taactgatgc ggccgaaggg atcttgcgag ttgtatccaa ctcaatcgca gaggggatac
     2341 gcctagcttc tgttcgcaaa ggactggatc cacgtgagtt cagtatggtg gcatttggag
     2401 gtgctgcagg gatcctggcc aatcaggtgg ccagagaagt tgggataaac aaagtgttag
     2461 tgcctcaggc tgcccctgtg ttatcggcat ttggtatgtt gacctcggag ctacgttacg
     2521 acttttctca atcatatcct gcggggttga atgtattgga tctcaaggga atgatttctc
     2581 gactgagaat tatggaaaaa cagggaaaag atcaactcag agaaaatggg gtgacagacg
     2641 aggaaataaa tgtagagttt agcgctgaca tgcgttactt agaccagata tatgaagtca
     2701 ctgttccaat tcctgatctg gatttggatg gtgctcgact caaagaggaa tgggctgaca
     2761 actttcacca acgatttgag gaattgtatt cttacaggca gtccgatcag gagattcggc
     2821 ttgtcacgct ccgggcgact gtatccgcac cactacccga aattgacgac agtgacagtg
     2881 cagataattc agtcgttgat tttgatcagg aagaggctgt actgagacag gtctatattg
     2941 gaagttggga aaacgttcct gttctcaaat ctgacagtat accgataggc caaaaagttc
     3001 ctggtcccgc tatcatcgaa tcagacttca ccactgttgt cttgaatcag tttaatgagg
     3061 ttacgaaaaa tgctgagggg acattaataa ttgggcttga agatcttgtg tcagatggtt
     3121 caatgggagg ggtggcagct ggatctgatc ctgtgaccct atcggtggtt gagaatcggt
     3181 tggagtctat agctattgag atgatggatg taatgcttcg tacttccatg tctcagattc
     3241 ttaattccag tcgggatttt tctacagcca ttttagatag tagatgtagg cttattgctc
     3301 aaggtgaggg gataccggta catgtgagcg ccttacccat agctggtgaa gctatattga
     3361 atacttttgg ggaggacatt catccgggtg atatgttcgc gttgaacgat ccatacaacg
     3421 ggggaagcca tttacctgac ataaccgtaa taaaacccgt gttcaagaat ggtgaactat
     3481 tattcttaac tatcaaccgc gcccatcaca gtgatgtcgg aggggctact catggcggtt
     3541 ataacccgtc
//
LOCUS       DASCNS010000132         2750 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001102164, whole genome shotgun sequence.
ACCESSION   DASCNS010000132 DASCNS010000000
VERSION     DASCNS010000132.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001102164"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="QF838_04640"
     CDS             <1..289
                     /locus_tag="QF838_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60008.1"
                     /translation="TASGEAQTLVTSAQDEVQTVIQEAQRRAYALVSDAENQAAELRQ
                     GADRYSMEVLSSIEEQLSNQLGQVRRGLDALNVTQTPKRTENNVPETSNSL"
     gene            complement(315..1166)
                     /locus_tag="QF838_04645"
     CDS             complement(315..1166)
                     /locus_tag="QF838_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HJO60009.1"
                     /translation="MTLEELILSQDKRGISKIRNSLARDFCFDAANFILANPGKVIIT
                     TGFYILSAEAAETDGPPGAIAIGNALEEIGYEVVYVTDKYCSQMMKEISTETSRVIEF
                     PIGTLGTSKKIASQILEEEKPDLLISIERCAPATDGLYRNMRDMDITSNTAKIDLLFE
                     THYASVGIGDGGNEIGLGNLYSEVEGSTELVGFPANTKTTKLIISSVSNWGGYGLIAA
                     ISILKGKNLLPSVESDIHNIKKIVAMGAVDGFSGDNVEKVDGFTLEENVSFLENLHDF
                     VQDSLAH"
     gene            complement(1175..1972)
                     /locus_tag="QF838_04650"
     CDS             complement(1175..1972)
                     /locus_tag="QF838_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HJO60010.1"
                     /translation="MTTIPEVALSPKDVRELIRGGEFTRPTAGVAAGYVQANLAILPK
                     EYAFDFLLFCQRNPKPCPLVEVLEPGQVEVSVTAPSSDIRTDVPLYRVYENGEMSAEI
                     SNLTGLWKSDFVSFLLGCSFSFESALINNGIRLPHFETGTNVSMYITNIPTVPAGPFS
                     GPMVVSMRSIPQEKVVRAVQVTSRFPGVHGSPIHIGDPSAIGISNLSQPDFGDPTIIK
                     EGEVPVFWACGVTPQSVAMSSKPPIMVTHAPGHMFITDMKDEDYSVL"
     gene            2106..2182
                     /locus_tag="QF838_04655"
     tRNA            2106..2182
                     /locus_tag="QF838_04655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2140..2142,aa:Pro,seq:ggg)
BASE COUNT          740 a          575 c          609 g          826 t
ORIGIN      
        1 aacagcttca ggcgaggccc aaacattagt tacaagcgct caagatgaag tccaaacagt
       61 cattcaagag gctcagcgta gagcatatgc attggtaagc gatgctgaaa accaagcggc
      121 tgagctgcgc caaggtgcag atcgctattc tatggaagtg ttatcttcta tagaagaaca
      181 gttgtctaat cagcttggtc aggtaaggcg tggcttggat gcgttaaatg tcacgcagac
      241 ccctaagcgg acagaaaata atgtaccaga gacaagcaac agtttgtaat tcgggttgaa
      301 atatataggc tgagttaatg agcaagtgaa tcttggacga aatcatggag attttccaaa
      361 aatgagacgt tttcctctaa agtgaatccg tctacttttt caacattatc tccactgaag
      421 ccgtccactg ctcccatggc gacgattttt tttatgttat ggatatcgct ttcgaccgaa
      481 gggagcaggt ttttaccttt caagatggaa atggcagcga tcaatccata gccaccccaa
      541 tttgagacgc tgcttattat cagttttgtc gttttggtgt ttgctgggaa gccaaccaat
      601 tctgtagacc cttcaacctc tgaataaaga ttacccaagc cgatctcgtt cccaccatct
      661 ccaataccaa cgctagcgta atgtgtctca aatagaaggt ctattttcgc agtattgcta
      721 gtgatgtcca tgtctctcat gtttctgtac agcccgtcag tagctggagc acacctttca
      781 attgatatca aaaggtctgg cttttcttcc tccaggatct gggatgctat ttttttactt
      841 gtaccaaggg ttccaatggg aaattcaatg actcgggatg tttctgttga aatttccttc
      901 atcatttggc tgcaatattt atctgtgacg tagacaacct catatcctat ctcttccaag
      961 gcattcccga ttgcgatcgc tccagggggg ccatcagtct cagcggcttc ggcacttagg
     1021 atgtaaaatc cagttgtgat gataactttt cctggattgg ctaatatgaa attagcagca
     1081 tcgaaacaga aatctctggc aagagaattt ctaatttttg aaatacctct tttatcctga
     1141 ctaagtatga gttcttctaa tgtcatggtt atatctatag gacagaataa tcttcatctt
     1201 tcatatcggt tataaacata tgccctgggg catgtgtaac cattatgggt ggttttgatg
     1261 acatagctac tgattgggga gtgaccccgc aagcccaaaa gactggcacc tcaccctctt
     1321 ttattatagt ggggtctcca aaatctggtt gggatagatt ggatatgcca attgctgacg
     1381 ggtctccaat gtggattggg gatccgtgca ctccggggaa tcttgatgtg acttgaacag
     1441 ctctcaccac cttttcctgt gggattgatc tcattgagac taccatcggc ccagaaaacg
     1501 ggcctgccgg aactgttggt atgttggtta tatacatgga gacgttggtg ccggtttcaa
     1561 aatgaggaag tcttatccca ttatttatta gggcggattc aaaagaaaag ctgcaaccta
     1621 acaaaaatga gacaaaatca gatttccata ggccagtaag gttacttatt tctgctgaca
     1681 tttccccatt ttcatacaca cggtacaagg gtacgtctgt tctaatatca cttgaagggg
     1741 ctgtcacaga gacttcaact tggccaggct ccaaaacttc aaccaacgga caaggtttag
     1801 ggtttctttg gcaaaaaagc agaaaatcaa aagcatactc tttcgggaga atagccaaat
     1861 tagcttgtac ataaccagcc gcgacacccg cagtgggacg tgtaaattcg ccgcctcgta
     1921 ttagttcccg aacatcttta ggtgacaagg ctacttcagg aatagtagtc atttgaaaca
     1981 tcttcctcca gtctactcgt ttgtaatata ggctgatggg aagtttaaca atacaatcaa
     2041 aactattcca caatggcata gtttattgat gcctttgtgt tggcatgcta ttcttttttg
     2101 tctgtcgggg agtggcgcag cttggtagcg cacgtgcatg gggtgcacgg ggtcgcaggt
     2161 tcgaatcctg tctccccgac caattattta taggttgatt taataaatct tattaattca
     2221 attaaggcgt gatttgaggc tctttgcctc atcctagaat ttcccgtcct atatttcccc
     2281 gatatgcacg tggttgtatg ctgactactt attttgaaaa gtacggttcc aaagttgtgg
     2341 tttgtatctt caagtaatag gggagatata ttaagatgta aatctgcttt acaggaatca
     2401 ttacaggatt gattgggttc ctgatagtca atgattggct tcttaacggg agtttttatt
     2461 aatccttgaa agaatacctc gtcgaatagt tcacattcag aaatgagacc acagacggcc
     2521 cctcctgtat acgattcagt gacgcagagg cgaagatttc ttgatcggag gaagctggtg
     2581 gcgaccattg caggagtgtc gtcatcttgc ccccagacaa aatctgcacc tacttattca
     2641 atgatttggt gctcaatttg gctgattaga gaggtggctt ccgaatctgt atctgatttt
     2701 gctattgctc taagatgtat gccatcttgt ttagaataaa taccgagata
//
LOCUS       DASCNS010000133         7323 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001102169, whole genome shotgun sequence.
ACCESSION   DASCNS010000133 DASCNS010000000
VERSION     DASCNS010000133.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001102169"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="QF838_04660"
     CDS             complement(<1..215)
                     /locus_tag="QF838_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60011.1"
                     /translation="MKNRNFVIALLYPMLMAVLAMGCGTSSAEPVSSNIPITATSVVV
                     AKTTSMPLEELIEEGDYQFESGCSGCHG"
     gene            complement(243..707)
                     /locus_tag="QF838_04665"
     CDS             complement(243..707)
                     /locus_tag="QF838_04665"
                     /inference="COORDINATES: protein motif:HMM:NF017394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HJO60012.1"
                     /translation="MKRVLSLIILGFFLWASCTSNAPLGGPPSIFYGEDVCDECGMII
                     SDSRFASGYTTKGGVSRKFDDIGGMFVFLKKNEDDAISLWVPDYGDQNWVKASEAYFV
                     VPGNIISPMGHGIIAFSDKQKASQTALKFEVEILSLKEIFDVVETHDSLHKH"
     gene            complement(704..1537)
                     /locus_tag="QF838_04670"
     CDS             complement(704..1537)
                     /locus_tag="QF838_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024091.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJO60013.1"
                     /translation="MNIFSVLVIARKELKDAMRNRWLALYASIFGTLSFSIAWLALSG
                     TSSYGSIGFGRTAASLINMVILIVPLMGLTLGALSIAGERDKKTMLYLLAQPVSKFEI
                     IIGKYLGLSLALIISLICGFGVSGIFITFKGEALQPTLYGAFILLTILLALVTLSFGF
                     LISVIVQKSVTAISAALFTWLFLVLFTDLGLMGTALILNLGINELFVLTLSNPLQVYK
                     ITAILTLRSSLEVLGPGGIYATRTYGSFLLPGLLTLLSMSVVIPLGLCYAVLCVKKDL
                     R"
     gene            complement(1534..3654)
                     /gene="nosD"
                     /locus_tag="QF838_04675"
     CDS             complement(1534..3654)
                     /gene="nosD"
                     /locus_tag="QF838_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HJO60014.1"
                     /translation="MKNIWRFTVIIATISFLMIGTNSTAHVAAEDIIVGYIESDQGRF
                     YALDDAIQAAQKGDTLHVFGGSYTSPIVISKSLTLVGHNQPVLDGEGRGTVVKITAPN
                     VLIQGFTILNSGASLDEENSGIAVEASGATVQDNQFRNTLFGIYLRKASNSTIRNNII
                     DTKRLDLPRRGDPIRVWYSNDVLIENNEVTEGRDVVLWYSERLIIRGNKVSGGRYGLH
                     FMYCDDATIDNNWLVDNSVGAFLMYSRRLRFHRNYVSNNRGPSGFGVGLKDMDDAIIR
                     DNLFVDNQIGASLDNSPREIDSTVLFEGNLFSFNNIGIKFTPSVRKNQFTNNSFEDNI
                     EQISIAGRTKFLDNIWSVNGIGNYWSDYAGYDADHDGLGDIPYESRKLFENVLETHPN
                     FRLFSFSPAAQSINLAAKALPIIRPKPKLIDKYPLMQISVSSATLIPTNSSPMSMTIL
                     SLSLFLVIGTLAYTPGLLTRGSDHHSDPLVNDFTLVVSNLTKRFGKIEVVKDICFSIL
                     PGESVALWGSNGAGKSTLIHCILGIIPAEGKVTIGNVDLANNSKPFRKLIGFVPQEID
                     FHPDLTVLETLDFYSGLRGVPKSVGEKLLKKLDLHDHSDKRIAQLSGGLKQRLALSLA
                     LLSDPPVLVLDEPTTNLDSDTRSNFLIYLNELKASGKTLIFASHRHEDVVHLADRVLV
                     MEKGLIVRDCSNREFSESMVGNRI"
     gene            complement(3651..4343)
                     /locus_tag="QF838_04680"
     CDS             complement(3651..4343)
                     /locus_tag="QF838_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HJO60015.1"
                     /translation="MESKITHTLDEQEIRSLLRRYHYLSSSLFIAAALVLMISIFLPY
                     WKLTLEAPQYPDGLEVNLFVNRVTGDVAEVDGLNHYIGMKPLDEAAKLERTMSIFAII
                     SLSLITASAILVHNKWAALLSLPVLLYPLIFVADLYFWLRLFGQNLDSTAPLSSSVEP
                     FTQPLMGTKVIANFVTYANFGSGFYLACIGVILLAVALYTHRRNYKPLTKAIQKGSYL
                     RQLSDTNSSDQQ"
     gene            complement(4435..6450)
                     /gene="nosZ"
                     /locus_tag="QF838_04685"
     CDS             complement(4435..6450)
                     /gene="nosZ"
                     /locus_tag="QF838_04685"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HJO60016.1"
                     /translation="MFSSTYVKAIPLGLIFVLCLTAIVLGCSDQDSNSAQLGSVDIPA
                     DIKEIADLRGLTPEDIEAAVKTYHPSGMHDEYIMMSSGGHSGQIHVIGIPSMRLLKTI
                     AVFTPEPWQGWGFGAEDTMEVLNQGNFGDTELRWGDTHHPAITETNGEYDGQFLFTND
                     KANARVAVIDLRDFETKQIIKNPIANNDHGSTMVTPNSEWVIEGGQYGAPLGSEYSSL
                     ENYADAYKGMITFWKFDRTAGRLNPDASFAMELPPYWQDICDAGKLASDGWVFCNSFN
                     TEMSTGGVENGNPPFEAGASQRDMDYLHIINLNKAVEVVAAGHTNTVNGFRVIPLETV
                     VEKDLLYFAPEPKSPHGVDVFPGGDYMVVSGKLDPHVTVYSFEKILTAIAAGPTQKDA
                     YGVPILDLDDVMAAQIEVGLGPLHTQFDNQGYAYTSLFLEPAVARWSMGGPYEDKHPE
                     SGWSLVSKIPVQYNVGHISAAEGDTVSPDGKYLVSLNKWAIDRFTPVGPLVPQNFQLI
                     DIAEPGDDMQLLYDMPVGFGEPHYAQIIKADKLNPWKVYPEVGWNPGTQSVDPHATIV
                     GEERIVRDGNNVEIFMTAIRSHFTPEQIEIKKGDHVTWHITNVERAYDATHGFALSGY
                     NINISIEPGESTTFEFDAVHDGVFSYYCTEFCSALHLEMTGYLLVQP"
     gene            complement(6733..7176)
                     /locus_tag="QF838_04690"
     CDS             complement(6733..7176)
                     /locus_tag="QF838_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012401.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJO60017.1"
                     /translation="MIRHLELPIMPVESRPNWYNLVINGKVPKERVFSAQEILDMPEI
                     IIKGDFFCNDGWMVKDLTWSGIPISQLLKLAEVNCKEVDSIKVCSDGYFKTFDIVEVL
                     QGNIIIATKLNGDFLSFGHGGPCRLIGGSRSGDNHVKWVKSIEVS"
BASE COUNT         2166 a         1599 c         1455 g         2103 t
ORIGIN      
        1 ccatgacatc ctgagcaccc actctcaaat tgatagtcac cttcttcgat caattcctca
       61 agaggcattg acgttgtttt tgctacaacc accgaagttg cggtaatagg tatatttgag
      121 gaaactggct ccgctgaaga ggtgccacaa cccatcgcta acacggccat taacatgggg
      181 tataaaagcg caattacaaa gtttctattt ttcatcaaac tcctttaaag atcgattatt
      241 tattaatgtt tatgcaagga atcgtgtgtt tcaacgacgt cgaaaatctc ctttaatgaa
      301 aggatttcaa cctcgaattt aagagcagtt tgactagctt tttgcttatc tgaaaaggct
      361 attattccgt gtcccatcgg agagataata tttcctggta caacaaaata agcctcacta
      421 gctttaaccc agttttggtc tccataatca ggaacccaaa gcgaaatagc atcgtcttcg
      481 ttttttttca gaaaaacgaa catgccgcct atgtcgtcga acttccgact cactcctccc
      541 ttagttgtat atcccgaagc aaatctactg tcactgatga tcattccaca ttcatcgcag
      601 acatcttctc cataaaaaat acttggaggg ccgcccagag gtgcattgct tgtacagcta
      661 gcccacagaa aaaaacccag aataataaga gatagaactc ttttcatctt agatcctttt
      721 ttacacatag cacggcataa cagaggccca ggggaattac tactgacata gataacaggg
      781 ttaataaacc cggtagcaaa aatgaaccat aagttcttgt ggcataaatt ccgcccggcc
      841 ccagaacttc aaggctactg cgcaaagtga gtattgcggt tattttatac acctggaggg
      901 gattggaaag agtaagaacg aatagttcat taatacccag attcaatatg agtgcggttc
      961 ccattagccc taaatcagtg aataaaacca aaaataacca tgtaaacaaa gcggcactga
     1021 tggccgtgac gcttttttga acaatcactg atataaggaa tccaaaacta agggtgacaa
     1081 gtgccagaag aatcgttaat aaaataaagg caccatacaa ggtaggctgg agagcttcac
     1141 ctttgaaggt tataaaaatg ccacttaccc cgaaaccgca gataagagag ataataagcg
     1201 cgaggcttag tccaagatat ttgccaatta ttatttcaaa tttagagact ggctgtgcta
     1261 gtaaatatag catggttttt ttatccctct cacctgcaat actcagggcg cctagggtca
     1321 atcccattag tggaacgatc aatatcacca tatttatgag gctagcagca gttcgcccaa
     1381 atccgatgga gccgtaagaa gaagtcccag aaagggcaag ccatgcgatc gaaaaggaca
     1441 atgttccaaa aatagatgcg tacagggcta accaccgatt tcgcatcgcg tcttttaatt
     1501 cttttctggc tatcaccagc acgctgaaaa tattcatatc ctatttccca ccattgattc
     1561 ggaaaactcc ctattggaac aatctcttac gattaggcct ttctccatca ccaaaaccct
     1621 atctgccaag tggaccacat cttcatgccg atgggatgca aaaataagag ttttaccact
     1681 ggcttttagc tcattaagat aaattaagaa attggacctt gtatcagaat caaggtttgt
     1741 ggtgggctcg tctaaaacta aaacaggtgg atcagacaga agggccagtg acaaggcaag
     1801 tcgttgttta agtccaccgg ataattgcgc tattcgttta tccgaatgat catgtaaatc
     1861 taattttttt aacagttttt ctccaacaga tttaggaacc cctcttaaac ctgagtagaa
     1921 atccaaagtt tctaatactg ttaaatctgg atgaaaatct atttcttgag gaacaaaccc
     1981 aattaatttc ctaaaaggtt tggagttatt cgccaaatct acattgccaa tagttacttt
     2041 accctctgca ggaataatcc cgagaataca atgaataagg gtagatttac ctgctccgtt
     2101 agagccccat aatgctactg actctcctgg taatatcgag aagcaaatat ccttaacaac
     2161 ctcaatttta ccgaaacgct tagttaggtt tgatactacc aacgtgaagt catttacgag
     2221 tggatcagag tggtgatcag atccgcgagt cagtaggcca ggggtgtagg caagggtccc
     2281 aatgaccaaa aacaaactta gcgacaggat tgtcattgac attggagagg agtttgtagg
     2341 aattaatgtt gctgaagaaa cggaaatctg catcagaggg tatttatcaa ttaattttgg
     2401 ttttggtcga attatcggta atgccttggc ggctaggttt atcgattgtg ctgcaggact
     2461 aaatgaaaat aggcgaaaat ttggatgagt ttccaacacg ttttcaaata acttacggga
     2521 ctcataaggg atatccccta gaccatcatg gtctgcatca tatcctgcgt aatcgctcca
     2581 ataattcccg atgccattga ccgaccagat attgtcaaga aatttagttc tcccagcaat
     2641 agaaatttgt tcgatattat cttcaaaact attattagtg aattggttct tgcggacgga
     2701 aggggtgaat ttgatcccta tgttgttaaa agaaaataga tttccctcaa ataaaacggt
     2761 gctgtcaatt tctctaggag aattatcgag tgatgcgcca atctgattat caacaaatag
     2821 attatctcga attatcgcat cgtccatatc tttgagcccc accccaaacc cacttgggcc
     2881 tctgttattg gaaacgtaat ttcgatgaaa ccggagccgt cggctgtaca taagaaaagc
     2941 acccacggaa ttatccacaa gccaattgtt atctatggtg gcatcgtcgc agtacatgaa
     3001 atgaaggcca taacgccctc ccgacacctt atttccacgg atgattaagc gttcggagta
     3061 ccacaggacg acatcccttc cttccgtgac ttcattattt tcgattaata catcgttgct
     3121 ataccaaacc cggatggggt ctcctcgtct tggtaaatcg aggcgctttg tgtcgataat
     3181 attatttcta atagttgaat tagacgcttt tcttagatat atcccaaaaa gggtatttct
     3241 aaactgattg tcttgtactg ttgctcctga cgcctctacc gctatccctg aattttcttc
     3301 atctaaactt gcaccactgt tgaggatagt gaaaccttga ataagcacgt tgggtgcggt
     3361 tattttcaca actgtacctc tgccttcacc gtcaagtact ggctggttat gtccaactaa
     3421 agtcaaagat tttgaaatca ctatcggaga ggtataagac cctccaaata cgtgcaaagt
     3481 atctcctttt tgggccgctt gaatagcatc atctagagca taaaaccttc cttgatcgga
     3541 ttcgatgtaa ccgacaatga tatcttctgc ggctacatgc gccgtactat tagtgcctat
     3601 catcaagaat gatatggttg cgatgattac tgtaaatctc catatatttt tcattgttga
     3661 tccgatgaat tcgtgtctga gagttgccgg agataggaac ccttctggat ggctttagtt
     3721 aaaggtttgt aatttctgcg gtgggtatag agtgctactg ccaaaagaat cacaccaatg
     3781 caggcaaggt agaaaccact tccaaaattc gcatatgtca cgaaattggc gattactttg
     3841 gtacccatta atggttgtgt gaatggctcc acagaggaac tcagaggtgc cgtcgaatcc
     3901 agattttggc cgaataacct caaccaaaaa tacagatctg ctacgaaaat tagagggtac
     3961 aacaacacag gtagcgataa aagagctgcc catttgttgt gtacgagaat tgctgaagcg
     4021 gtgattaaag ataacgaaat aatcgcaaaa atactcatgg tccgttccag tttcgcagcc
     4081 tcatctaaag gtttcatccc tatgtaatga ttcagcccat ctacttccgc aacgtctccg
     4141 gtcacacgat taacgaacag gttaacttca agtccatcag ggtattgagg tgcctccaaa
     4201 gtcaatttcc agtatgggag aaaaattgag atcatcaaaa caagcgctgc agctataaag
     4261 agtgaagaag aaagatagtg atatctcctc aaaagagatc ggatttcttg ttcatctaat
     4321 gtgtgtgtaa ttttgctttc cattgcctca aaccacatct tttgttttta agggcagatt
     4381 aatgggattc agaccagacc acgtggtctg gtctgatccc attaattcca tgttttatgg
     4441 ttgcaccaat agatagcctg tcatctctaa atgtaatgca gagcaaaact ctgtacagta
     4501 gtagctgaaa acaccatcgt gaacagcatc aaactcaaac gtggtggatt ctccaggttc
     4561 aatactgata tttatgttgt atccagaaag agcgaatccg tgagtagcgt catatgctct
     4621 ttccacgttg gttatatgcc atgtgacatg atctcctttc ttaatttcaa tttgctcagg
     4681 agtaaaatgg cttcgaatgg ctgtcatgaa tatttccaca ttgttcccat ctcgaacaat
     4741 tcgttcttct ccaacaattg ttgcgtgagg atcgaccgat tgggttccag ggttccatcc
     4801 gacttctggg taaactttcc aaggattcaa tttatcggcc ttaatgattt gagcgtaatg
     4861 tggttcacca aagcctacag gcatgtcgta tagcaactgc atatcgtccc caggttcagc
     4921 tatatcgatc aactggaagt tctgaggaac taaaggccca acaggcgtaa aacgatcgat
     4981 tgcccattta tttagcgaaa ccaggtattt tccatccgga ctcacagtgt caccttcagc
     5041 tgcagagatg tgcccaacgt tgtattgaac aggaattttg gataccaaag accagccact
     5101 ctcgggatgt ttgtcttcgt aaggcccacc cattgaccac cgggcgacag ctggctccaa
     5161 aaacaagctg gtataggcat aaccttgatt gtcgaattga gtatgcaatg gccctagacc
     5221 aacctctatc tgagcagcca taacgtcatc taaatccaat attgggacac cgtaggcatc
     5281 cttttgggtc ggtcccgcgg ctattgcagt taatattttc tcgaagctgt aaacagtcac
     5341 atgtggatcc aattttcctg aaacgaccat ataatcacca ccagggaata catccacccc
     5401 atgaggactt ttcggttctg gagcaaagta aaggagatct ttttcaacta cggtctccaa
     5461 tggaatcaca cgaaagccat taacggtgtt ggtgtgtcca gcagcaacga cttctacggc
     5521 tttatttaaa ttaattatgt ggaggtaatc catatctcgc tgtgaagctc cagcttcaaa
     5581 tggaggattt ccgttttcta ctccccccgt actcatctca gtattgaatg agttgcaaaa
     5641 tacccagcca tcgcttgcga gtttcccagc atcgcatata tcttgccagt aaggaggtaa
     5701 ttccatagca aaagaggcgt ctgggttaag tcgtccagca gttcggtcga atttccagaa
     5761 agttatcatt cctttataag catcggcata gttttcaagg gaactgtact cagaacccag
     5821 aggggctccg tattggcccc cttcgattac ccattctgaa ttcggagtaa ccattgtgga
     5881 tccatgatca ttatttgcaa tagggttttt gataatttgt ttggtttcga aatcccttaa
     5941 atctattacc gcaactctag cgttggcttt atcgttggta aaaaggaact gtccatcata
     6001 ttctccgttg gtttcggtaa tagcgggatg atgagtatcg ccccatcgta gctctgtgtc
     6061 accaaaattc ccctggttta agacttccat agtgtcctcg gctccaaaac cccaaccttg
     6121 ccacggctca ggagtgaaca ctgcaatcgt ttttagcagc ctcattgacg gaattccaat
     6181 aacgtgaatt tgacctgaat gtcctccaga agacatcatt atgtactcat catgcatccc
     6241 tgaaggatga tatgttttta ctgcagcctc aatatcctcc ggggtcaaac ctctgagatc
     6301 cgctatttct tttatatctg ctgggatatc tacagatccc aattgagctg aattagaatc
     6361 ctggtcagaa cagccaagca ctatcgctgt taaacacaga acaaaaatta gacctagggg
     6421 aatcgctttg acataggtac tggaaaacac tggtgatctc cttttgaata tgagttccta
     6481 ataagttctg aattaatgtg cacctaaatc catgtcgatc gcataaaata aatatctatt
     6541 ttgtgaatat aatgtgagca cggtattgga taaaacaata attagggata tttgaaaaat
     6601 gctgaaggta ggttgacagg caataatatt gatgatctat taaggggagg aaaacaaccg
     6661 gaccgtcttt ttagaaacgg tccaggctcg gagtatgtct acttaggcga atgcatagag
     6721 aagtcgctgc tgtcagctca cctcaatact tttcacccac ttaacgtgat tgtcaccact
     6781 gcggctaccg ccaatcaacc tacaagggcc tccgtggcca aatgaaagaa aatctccatt
     6841 caactttgta gcaataatta tgttgccttg aaggacttcc actatatcga aagttttgaa
     6901 gtaaccatcg ctacaaactt taatggaatc tacttctttg cagttaacct cggccaattt
     6961 taagagttgt gatatcggta tacccgacca agtgaggtct ttgaccatcc acccgtcgtt
     7021 acaaaaaaaa tctcccttta ttattatctc gggcatatct aaaatctctt gagcagaaaa
     7081 aaccctctct tttggtactt tgccatttat tactaagtta taccaatttg gacgtgactc
     7141 aacaggcata atcggtaatt ctagatgccg gatcattatt atccccctta tcttaatggg
     7201 aaggtgttat ccttgaattc aagattgatt agcgaagata tttaaggaca agatatcgtg
     7261 tcactatgac actcaaataa tcccatttac tgtatttgat gtgagtttat tatgatattt
     7321 gta
//
LOCUS       DASCNS010000134         9342 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001120767, whole genome shotgun sequence.
ACCESSION   DASCNS010000134 DASCNS010000000
VERSION     DASCNS010000134.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001120767"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1..97
                     /locus_tag="QF838_04695"
                     /pseudo
     CDS             1..97
                     /locus_tag="QF838_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015211512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            124..1542
                     /locus_tag="QF838_04700"
     CDS             124..1542
                     /locus_tag="QF838_04700"
                     /inference="COORDINATES: protein motif:HMM:NF020390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO60018.1"
                     /translation="MESTSRVLAAHLARRAGFGATLEELDTYSEMEYEDLVEHLITKI
                     DSSHISDHLIFRRHVDLHTMQGHNAVYWSYRMISTDNPFIEKMALFWHGVFATAENKL
                     NNIGSLTNQIDMFRRHGIGRYDDLLVELSKDPAMLIWLDNHTNHKDSINENYGREILE
                     LFSMGVGNYTEDDIKECARAFTGWTVKNGEYLSMMAVKDSIWPYGRILWHHEYRATDH
                     DTGEKRFLGESSNFNGDGVIDVICRQRATANFVARHLYNFFVADEVPVPQWSSTPPKD
                     PEAINILANSYMESNHDIRSIMRILFNSDFFKDSKFKRVKSPAELVIGTLRNTREYQV
                     PDGGEIGIYDIGEESGYMGQKLLDPPSVEGWHTGEEWITSGSLVDRVNFATSHLGDDS
                     NPGVKNLIERVGFENKQPGKLIDDCLKTLGYVEIETDTYQKLEEVAAAGLKQSDSSDG
                     VSSNLIIELFKLIVSSREYQRC"
     gene            complement(1555..2601)
                     /locus_tag="QF838_04705"
     CDS             complement(1555..2601)
                     /locus_tag="QF838_04705"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005282692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HJO60019.1"
                     /translation="MRIESDLKLDYKDVLLKPKRSKLTSRKDVDMEREFKFLNSGKEF
                     TGIPIIASSMDGVGTFSMAKILQNYKMLTVLRKHYEPENWDNAVASGLDLNYVSICTG
                     TNRIWDEDASDFLTLKVVLEKYPEIPFITIDVANGYHENFVDFVGQIREEFPEQTIIA
                     GNVITAEMTEELILRGADIVKCGIGPGSVCTTRLMTGVGIPQFSGIIECADAAHGVGG
                     QVIADGGCVYPGDVAKAFGAGSDFVMLGGMLAGHEESEIDILDGKMEFYGMSSDRAME
                     IHGARKDGYRGAEGKEIRIPYKGKVEDTMTEIIGGVRSACTYIGATKIKDMPKCATFV
                     QVLQQENQVFNLFE"
     gene            2786..4351
                     /locus_tag="QF838_04710"
     CDS             2786..4351
                     /locus_tag="QF838_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013225.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HJO60020.1"
                     /translation="MVWQNEINELNKRLEMAHGMGGQEGIDRQHSNGKLTVRERVQAI
                     SDPGTFKELSALAGSARYKNNELVDFTPYPRVIGTARIGGMRVMLDGGDFTVRGGAGE
                     RGTGSQPSSLKYALQWRIPLVRLLDAVGGSVRTFEQIGRTYLPDGGGTSKAVDLLNEV
                     PVVSAVLGSVAGLPAIEACLCHFSVMVKDTSQVFAGGPPVVKAALGYDVTKEDLGDHN
                     SQVFKTGVIDNLADSEEEAFKMVRQFLSYLPSSVWKKPQRLDTQDDSARKEDELLSII
                     PRRSKRKYDPYKILEYVMDRDSFFEIAPFYGQSKITGLARVNGYPVGVLMNNPNQLGG
                     STDVASGEKTIRLVSLCETFHLPIVSFVDEPGFMVGLDAQNQGIVRAGARLVIANARS
                     QTPWISFYIRQAYGVAGQTNHRPTGMYHRYAWPSAKWGSMHIEGGVSAAYRREIAASE
                     NPEQKRLEIEARLQAMASPFRTAEDTGGESSSHGIDIIDPRDTRPLLCDFVDMAQDML
                     DTQLGPPTSPYIP"
     gene            complement(4329..5582)
                     /locus_tag="QF838_04715"
     CDS             complement(4329..5582)
                     /locus_tag="QF838_04715"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60021.1"
                     /translation="MISITRLYITRIFSSFKIKKYRYLWASDCLQAWAEYMELIVLSW
                     LILEISSSPLLVGLYGALRFMGTIMSPIFGVVVDKFDRKILLVLIRGSFALNGIVILC
                     IAFAGVLTTTAILIMAGFLGLSKTFDMVIRQSILPAVVGDKNLNNAVALSRAGGDVTQ
                     MIGPIVGGVVLAFLSVRVSYGIIVGLYFISLFCAISIGEISVKSSAGTLNVINNFKIG
                     MKFIQQTPVVAGLLVLAVIINLATFPIYFGLISVLAKEVFDSTSTGLGFMMGSYSCGA
                     VLGSLIAGGRDGSGKIGRFSIGGAALWSGAIILLALVPNFVSSLPVLFLAGLGQSLCV
                     VAIAMMLLSLTPDNLRGRVMGLRQLAVYSLPLGLLISGTIAEMWGVKVAIFLDGLMGI
                     LLVTGCVAIWPTLLNASQPKEYKEK"
     gene            5688..6701
                     /locus_tag="QF838_04720"
     CDS             5688..6701
                     /locus_tag="QF838_04720"
                     /inference="COORDINATES: protein motif:HMM:NF025957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HJO60022.1"
                     /translation="MGKSINPDDTRLKWKEAVSTETTTEGVMPWRIPFAQKDLFAWQL
                     VDRAAMPAGVRLEFRSNTSYLEMFFDSIFFDSIPDRSPVDIFCDGELLATLPTANLNF
                     LRVENLGSKNKAIQIWLPQWGELRIKKLVIEDNAILTEPEARKHPEWVTYGSSITQCR
                     AADSPSQTWPALVARKNHLELTCMGYGGQCHLDPMIARTIRDLPADIISLCLGINIYS
                     ASSLNQRTFQPGILGFVQILREKHIDAPIIVMSPIYSPGREETFNEVGMTLQLMRSEI
                     ANSVDILKGLGDMHIHYVDGLDIFDNQYENLLPDNLHPNNEGYSIMASNISNFLAPYL
                     CLE"
     gene            6760..7530
                     /locus_tag="QF838_04725"
     CDS             6760..7530
                     /locus_tag="QF838_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60023.1"
                     /translation="MRLKNKVALISGGANGVKNELMGFGGACAWKFVQEGAKVVIADI
                     DTANGQKTVDQIKAEKGQVEFIKLDVTKDSDWKTAVDFAVHTFNQIDILVNCAGTASV
                     ERVEETTEDIWDAQMDIHAKGAFLGTQAVIPIMIKQGQGSIIHLSSINGLIGSQSSTA
                     YHAAKGAVRLLAKNTAIQYAKDGIRVNSVHPGYADTPLTKPLFADEDALALRLNKVPL
                     GRIGTAGEIANGIIYLASDESSFVTGSELVIDGGITAQ"
     gene            7532..7960
                     /locus_tag="QF838_04730"
     CDS             7532..7960
                     /locus_tag="QF838_04730"
                     /inference="COORDINATES: protein motif:HMM:NF016056.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HJO60024.1"
                     /translation="MSTPEVAFDSFLICFHGHCNLCNSWVDFLIKRDRKDVFTFTSLQ
                     SNGGRNVLLSHGYSESNLESLDNIVALNAGQVSMRSDAVIEILVALGGIYKLARFLKL
                     IPSSFRNFVYDTVAKRRYRWFGRKDICRIPSTEESQKFLE"
     gene            7953..8612
                     /locus_tag="QF838_04735"
     CDS             7953..8612
                     /locus_tag="QF838_04735"
                     /inference="COORDINATES: protein motif:HMM:NF021371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HJO60025.1"
                     /translation="MSRSNTKKRPVFLRAKWNGNTFLSLVAFRFSNTRMLGIPTFGWR
                     NFDEVNLRFYVRGTENQKNKNGVVFIKEIVPRWLVSTIARLLYRENYQNKDMNNSIES
                     RPDSSKIISYEIRESDLSNKFSAFVTTPEKMPTPDSLEAFITEHYWGFSSRNSEKTME
                     YEVEHPQWTVSKVEDYSITFDFEKVYGHRFSFLSDQNPHSVFYCEGSDIIVRMGKTLN
                     F"
     gene            complement(8602..>9342)
                     /locus_tag="QF838_04740"
     CDS             complement(8602..>9342)
                     /locus_tag="QF838_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014298.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HJO60026.1"
                     /translation="FKGIKIPKPWIVKTATLGYDGHGQWKIENDTQERELLKSIHGES
                     WVIESVVDYRLEMSVIIVSNGKGNLVAYPPTENTHQGGILRMSISPPRLKTEIQNKAI
                     DLAKEVIGCLDDAGVFCVEMFLTATDEILVNEIAPRPHNSGHHTIDAFSISQYESQVR
                     ALVGLPVIEPDFLNASVLLNILGEEADILSDTDHKFELYSDSSARLYMYGKQSVRPRR
                     KMGHVMFLGGSVEDLISKAENTLMALKN"
BASE COUNT         2951 a         1962 c         1944 g         2485 t
ORIGIN      
        1 cggtgtgggt tgtgagatgg atacaattat ggaaattgca aataaatatg atatccctgt
       61 agtagaagat aatgctcatg gtctatttgg gaaatatagt taaaaagaag atacgaggca
      121 aaaatggagt caacaagtag agtgttagca gcgcatttgg caagaagggc tggatttgga
      181 gccacccttg aagagctaga tacctacagt gagatggaat atgaagattt ggttgaacat
      241 ctaataacaa agatagactc ctctcatata tccgatcatc tgatttttag gaggcacgtt
      301 gacctccata ccatgcaggg acataacgcc gtctattgga gttataggat gatttcaact
      361 gacaatcctt tcatagaaaa aatggccctg ttctggcatg gagttttcgc aaccgctgaa
      421 aacaaattaa acaacatagg atcattaaca aaccaaatag atatgtttag gagacatggt
      481 attggcagat acgacgatct tttggtcgag ttgtcaaaag accctgcaat gctcatctgg
      541 ctcgacaacc acaccaacca taaagattcg atcaacgaga attatggtag agaaatctta
      601 gaactcttct ctatgggggt agggaactat accgaggatg acatcaaaga gtgtgcaaga
      661 gcttttacag gatggactgt gaagaacggg gagtacctgt ccatgatggc agttaaagac
      721 tccatatggc catatggaag aatcctatgg caccatgaat acagagccac tgaccacgat
      781 accggtgaga aaagattttt gggtgaaagc tcaaatttca atggtgatgg tgttatagat
      841 gtgatctgca ggcaaagagc caccgctaat ttcgtggctc ggcatttata taatttcttc
      901 gttgcagatg aggttccagt accgcagtgg tccagtactc cgccaaaaga tcctgaagca
      961 ataaatatcc ttgcaaattc atacatggaa tccaaccacg atataaggtc tataatgagg
     1021 atattattta attccgattt cttcaaagac tctaaattca aaagggtcaa aagccctgct
     1081 gaattggtta ttggcactct cagaaacacc agagaatacc aggtaccaga cggcggagaa
     1141 ataggtatat acgacatagg ggaagagtcg ggttatatgg gtcagaaatt gctggaccca
     1201 ccttcggtcg aaggttggca tacgggggaa gaatggatca ctagcggctc tctagtagac
     1261 cgggttaatt tcgctacaag ccatcttggt gatgattcca atccaggagt taaaaacctc
     1321 atagaaagag tagggttcga aaataagcaa cccggcaagt taatagatga ttgcctaaag
     1381 acccttggtt atgttgaaat cgaaacagac acttatcaaa aattagagga agtggcagct
     1441 gctggattaa aacaatctga ttcttctgac ggagtctctt ctaatttgat tatcgaacta
     1501 ttcaaattaa tagtctcgag tcgggaatac cagcgatgtt aaaaaagaat caacttattc
     1561 aaaaaggtta aacacctggt tctcctgctg aagaacctgt acaaaggtgg cacattttgg
     1621 catgtcctta atttttgtgg caccaatgta ggtgcatgct gatcgaaccc ccccaatgat
     1681 ttctgtcatt gtatcctcga ctttcccttt gtaaggaatt ctgatttcct tcccttcggc
     1741 accacggtat ccatcttttc gtgctccatg aatttccatg gccctatcag aactcatgcc
     1801 ataaaattcc attttgccgt ctaaaatgtc aatctcagat tcttcatggc ctgccaacat
     1861 acctcccaac ataacaaagt cagagcccgc cccaaaagcc ttggcaacat ctcccgggta
     1921 tacacaacca ccatctgcaa ttacctgacc gcccactcca tgagcggcat ctgcacactc
     1981 aataattcca gaaaattgag gaattcccac cccggtcatc aaccgtgtcg tacatacaga
     2041 acccggccct atcccacatt taactatatc tgcgccccgc aagataagct cctctgtcat
     2101 ttccgcagta attacattcc cggcgattat ggtctgttcg ggaaattcct ctctgatttg
     2161 ccccacaaaa tccacaaaat tttcgtgata tccattggct acatcaatag ttataaaggg
     2221 aatttctggg tacttttcca gaacaacctt aagagttaaa aagtcggaag catcctcatc
     2281 ccaaattctg ttggttccag tacaaatgga tacgtaattc agatctagcc cgctggcaac
     2341 tgcattatcc cagttctctg gttcgtaatg ttttctcaaa acagtcaaca ttttgtaatt
     2401 ctggagaatt tttgccatcg aaaaagtacc aacaccgtcc atactggaag cgataattgg
     2461 aatccctgtg aattctttac cagaatttaa aaacttaaac tctcgttcca tgtcaacatc
     2521 ttttctggat gttagtttag aacgttttgg tttcaataaa acatccttgt aatcaagctt
     2581 taaatcggat tctattctca ttaattatca ccgttgaccc attgtatgac tgcaagtctt
     2641 tacagtaact cgacaattaa ttctaaccat acgaacgaat gaataatagg tccctcagtt
     2701 taccaatgat ggtcttacaa gttgtatggt ttgaaaaacc actataaagt tcgcgaatct
     2761 atagcatcat aaggatcatc caaatatggt ttggcaaaac gaaataaatg aactcaacaa
     2821 aagactggaa atggcccacg gaatgggtgg tcaagaaggc atagataggc agcattctaa
     2881 cggtaaacta accgtccgcg aacgtgtaca ggccatttct gatccaggga cctttaaaga
     2941 attgtcggca ttagcaggca gcgctcgcta caaaaacaac gaattagtag atttcacccc
     3001 ataccctaga gtgataggca cggcacgtat tggtggtatg cgagtgatgc tggatggtgg
     3061 ggatttcacc gttcggggtg gggcagggga gagaggcact ggtagccaac caagctcact
     3121 aaaatatgct ttgcaatggc gtattcccct agttcgttta cttgatgcag tgggtggcag
     3181 cgtaagaaca tttgagcaaa tcggtaggac ctatttacca gatggaggtg ggacttctaa
     3241 agccgttgat ttattgaatg aagtccctgt agtctcagcc gtcttgggat ccgtagctgg
     3301 attacctgca attgaagcgt gtctttgcca tttcagcgtc atggtaaaag atacgagtca
     3361 agttttcgcg ggaggacccc ctgtggtaaa ggccgctttg ggctacgacg ttactaaaga
     3421 ggatttagga gatcacaaca gccaagtttt caaaactgga gtaatcgata acctagctga
     3481 ttcagaagaa gaagctttca agatggttcg tcagtttctc agctatctgc cgtctagtgt
     3541 ctggaaaaaa ccacaacgtt tagacaccca ggatgactct gctagaaagg aggatgaact
     3601 tctgtccatt attcctcgaa gaagcaaaag aaaatatgac ccttataaaa tattggaata
     3661 tgtaatggac cgcgattcat tttttgaaat tgcaccattt tacggccaat ccaaaataac
     3721 gggtttagca agggtaaatg ggtatcctgt aggggtatta atgaacaacc caaatcaatt
     3781 aggtggttca accgacgtag cctcagggga aaaaactata aggttggtaa gcctctgtga
     3841 aacgttccat ttgcccattg tttcgtttgt ggatgaacca ggcttcatgg tggggctcga
     3901 cgctcaaaac cagggaatag tcagagctgg ggcaagattg gtaattgcaa atgctcgaag
     3961 ccaaacacca tggataagtt tttatataag gcaagcatac ggtgtcgccg ggcagacaaa
     4021 ccacagacca acaggaatgt atcacagata tgcgtggcct tcggcaaaat ggggttctat
     4081 gcatattgaa gggggagtgt ccgcagcata tcgaagagaa atagctgcct cagaaaaccc
     4141 agaacaaaaa aggctggaga ttgaagcacg tcttcaagca atggcatctc cattcagaac
     4201 tgctgaggac acgggaggag aaagcagttc acacggaatt gacataatag accctagaga
     4261 tactagacct ctattgtgtg acttcgtgga catggcgcaa gacatgcttg atacccaatt
     4321 aggtcctcct acttctcctt atattcctta ggctggctag cattcaataa cgtaggccag
     4381 atagccacgc aacccgtcac aagtaaaata cccattaacc cgtctaggaa tattgccacc
     4441 ttcacccccc acatttcagc aatcgtaccg gaaatcagta ggcctagcgg caaggaatat
     4501 acagcaagct gcctcaaacc cattactctt cctctcaaat tatctggggt cagtgacaaa
     4561 agcatcatcg caatggcaac cacacacagc gactgtccga gccctgccaa aaataaaacg
     4621 ggcagtgagg aaacaaaatt gggaaccaat gctaataaaa tgatggcacc actccataga
     4681 gcagctcctc caatggagaa tctacctatt tttcctgacc catctctacc tccggcaatg
     4741 agggagccta aaaccgctcc acaagaatag ctccccatca taaaccccaa acctgtcgag
     4801 gtggaatcga aaacctcttt cgccaataca gaaatgagcc caaaatagat agggaaagta
     4861 gctaaattga taatcactgc caaaaccaga agtcccgcaa caactggagt ctgctgaata
     4921 aatttcatcc cgatcttgaa attattaatc acatttaaag tacctgcaga agatttcaca
     4981 gatatctctc ctatgctgat agcgcaaaaa agggatataa aataaagccc tactataatt
     5041 ccgtagctga ctcttacact caggaaagct aggacaacac cgcctactat aggtcctatc
     5101 atttgagtta catcaccccc cgcccttgat aaggctacag cattattcaa attcttatcc
     5161 cctacgacag caggtaggat tgactgacga ataaccatat caaacgtttt tgaaagtcca
     5221 agaaacccgg ccatgattaa tatggctgta gtggtcagaa cacctgcaaa tgcaatgcac
     5281 aagataacta ttccatttaa agcaaatgat cctctgatca acactaagag aattttccgg
     5341 tcaaacttat ccacaacgac tccaaaaatg ggggacataa tagtacccat aaatctcaag
     5401 gcgccgtaaa gtcctacaag aagtggcgag ctggatattt ctaatatcag ccaggacaga
     5461 actatgagtt ccatatattc cgcccaggcc tgtaaacaat cggacgccca aaggtaacgg
     5521 tattttttta ttttgaagct actgaaaatt ctggttatat atagtcttgt tatgcttatc
     5581 atctttaaaa cggtaccaat atctggaaat acagccagca aatatatacc cttacccaca
     5641 tgaatgcacc tgtcacgaca acataacctc ttaaaggtaa ttcaagaatg ggcaaaagca
     5701 taaatcccga tgacacaaga ctgaaatgga aagaggcagt ttcaacagag actaccacgg
     5761 aaggagtcat gccttggaga atccctttcg ctcaaaaaga tctattcgca tggcaacttg
     5821 tggaccgtgc tgcaatgccc gctggtgtac gacttgaatt taggagcaac acctcgtatt
     5881 tagaaatgtt ttttgattcc atattttttg attccatacc ggatagaagt ccagtagata
     5941 tattttgtga cggagaatta ctggctacat taccgacagc caatctgaat tttctgagag
     6001 tagagaattt aggttcaaaa aataaagcaa ttcagatttg gttaccacag tggggagaac
     6061 taagaattaa aaaattggta atagaggaca atgcaatatt gactgaaccc gaggcgcgga
     6121 aacacccgga atgggtcacc tatggaagct ctattactca atgtagagct gctgattcac
     6181 ctagtcagac atggccggcg ttagttgctc ggaaaaacca tctggaattg acatgtatgg
     6241 gatatggggg acaatgccat ctagatccaa tgattgcccg tacaatccga gatctccccg
     6301 cagatataat ctctctttgt ttaggcataa atatatatag tgcatcttca ttgaatcaac
     6361 gtacatttca accaggtatt ttgggatttg ttcaaatttt gcgagaaaaa catatagacg
     6421 ctcctatcat agtaatgtcg ccaatttatt cgcctggtag agaagaaacc tttaatgagg
     6481 tgggaatgac ccttcagcta atgagaagtg agatagcaaa ctctgttgat attttaaaag
     6541 ggctgggaga tatgcatatc cactatgtag acggtttgga tatattcgat aaccaatatg
     6601 agaatttgct tcctgacaac ctgcatccaa ataacgaagg ctattcgatt atggccagca
     6661 atatttcgaa tttcctggct ccatatttat gccttgaatg attagttagt aataaaaagc
     6721 aacttatatg aagaaaataa gtcgaaaggg gtaaatcata tgcggctaaa aaacaaagtt
     6781 gcgttaataa gtggcggggc caatggtgtg aaaaatgaat taatgggatt cggtggagcc
     6841 tgtgcgtgga aatttgtcca agaaggggca aaagtcgtga ttgcggacat cgatacagcc
     6901 aatggacaga aaaccgtaga ccaaataaaa gcggaaaaag gtcaggttga atttataaaa
     6961 ttggacgtga cgaaagattc ggactggaaa acagcagtag acttcgcagt gcataccttt
     7021 aaccaaatag acattttggt taactgtgct ggtaccgcat cagttgagcg ggtagaagaa
     7081 accaccgaag atatctggga tgcccaaatg gatatccatg caaaaggagc attcttaggt
     7141 acacaagctg tcattccgat aatgatcaaa caaggtcaag ggtcaataat tcacttatcg
     7201 tcaataaatg gcctgattgg tagccagagc tctacggcat accatgccgc taagggagca
     7261 gtgagactgc tggcaaaaaa tacggctata cagtatgcaa aagacggaat aagagtgaac
     7321 tcagttcatc ctggatatgc cgatactcca ttaacgaagc ctctgttcgc tgatgaagat
     7381 gcattagccc tgaggcttaa taaggttcca ttaggtagga ttggaacggc gggtgaaatt
     7441 gccaacggaa taatttattt agcatctgat gagtcttctt tcgttactgg ttcagaatta
     7501 gtcatagatg gcggaataac tgctcagtga aatgagcacc ccagaagtcg catttgatag
     7561 ttttttgata tgttttcatg gtcattgtaa tctgtgtaat tcatgggttg attttctgat
     7621 caaacgagac agaaaagatg tttttacctt cacttccctc cagtcaaatg gaggtagaaa
     7681 tgtattactc agtcatgggt atagtgaaag caaccttgaa tccttggata acatagtagc
     7741 cctcaacgca ggccaagtat ctatgagatc ggatgctgtg atagaaatct tggttgcctt
     7801 aggtggaatt tataagctag caagattcct taaattgata ccatcatcct tccgaaactt
     7861 tgtctatgac acagtagcaa aaagacgata tcgatggttc gggagaaaag atatctgtcg
     7921 cataccctcc acagaggagt cgcaaaagtt tcttgagtag atcgaatacc aaaaaaagac
     7981 cagttttcct tcgggccaaa tggaatggca acaccttctt aagcctggta gctttccgtt
     8041 tttccaacac tcgtatgtta ggaataccca ccttcgggtg gagaaatttt gacgaggtta
     8101 atctaagatt ttatgtacgg ggaacagaaa atcaaaaaaa caaaaacggt gtcgtattca
     8161 ttaaagaaat tgtcccacgc tggttagtga gcactatagc tagattactt taccgtgaaa
     8221 attaccaaaa caaagacatg aataattcta ttgaatcgag gccagattcg agtaaaatca
     8281 tcagctacga aataagggaa tcagatttaa gcaataaatt ttctgcattt gtgactaccc
     8341 cggagaaaat gccaactcct gatagtttag aggcattcat aacagaacat tattggggat
     8401 tctctagtag gaactctgaa aaaacaatgg aatacgaagt agaacatccg caatggacag
     8461 tctcaaaagt cgaggactat tcaatcacat ttgactttga aaaagtctat gggcacagat
     8521 tttcattcct ttctgaccaa aacccgcatt ctgtattcta ttgtgaaggg tctgacataa
     8581 ttgtgagaat gggaaaaaca ctcaattttt aagtgccatt aaagtatttt ctgccttgct
     8641 aatgagatct tccaccgaac ctcctagaaa cataacatgt cccatttttc ttctagggcg
     8701 caccgattgt ttaccataca tatataacct tgcagaactg tcagaataca attcgaattt
     8761 atgatcagta tcgctcaata tatccgcttc ttctcctaga atattaagta aaaccgaagc
     8821 attcaaaaag tctggttcta taactggtaa tcccactaaa gctcttacct ggctctcata
     8881 ttgtgaaata ctgaaggcat ctatagtgtg gtgacctgaa ttatgtggcc ttggtgctat
     8941 ctcattcaca agtatttcat ctgttgcggt taagaacatc tcaacacaga aaactcctgc
     9001 atcatctaaa catccaatta cttctttcgc gagatctatg gccttatttt gtatctcagt
     9061 ttttagtctt ggaggagaga tactcatccg aagtatacct ccctggtgtg tattttccgt
     9121 tggagggtat gcaaccaaat ttcctttgcc attcgaaaca attatcacag acatctcaag
     9181 tcgataatca acaaccgatt caataaccca agattcacca tgaattgact ttaacaactc
     9241 tctctcttga gtgtcatttt caattttcca ctgtccgtga ccatcgtagc ccagtgttgc
     9301 tgtctttacg atccacggct taggaatttt tatacccttg aa
//
LOCUS       DASCNS010000135        10209 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001124941, whole genome shotgun sequence.
ACCESSION   DASCNS010000135 DASCNS010000000
VERSION     DASCNS010000135.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001124941"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="QF838_04745"
     CDS             complement(<1..345)
                     /locus_tag="QF838_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60027.1"
                     /translation="MPVKTQDAGTAGKNVTNPLTHYKNIQEITESYVDGDETPVGVVQ
                     HFLARIDSFDSSYLSYATVMADQALEQAEILSRELGEGGEIRRTNWEDEDNGVIGHGF
                     IWPVQMIEGPNDT"
     gene            626..1657
                     /locus_tag="QF838_04750"
     CDS             626..1657
                     /locus_tag="QF838_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO60028.1"
                     /translation="MSNGDQNWEFGVKDHGHSVVDETDMAPAVLGEGNFCYQVSGQNW
                     GTLPDGWVYKEATAVDVDSKDRVYVFNRGTMPMIVFDPEGNVIDTWGNGVFSNPHGVT
                     IGPGDEVYCVDNGDHTVRKFTSDGKLLLTVGTPGKSAAPMSGIPFARPTHVGVEPNTG
                     NFYVADGYTNASVHKFSPDGKLLFSWGESGTNPGQFNIVHGVNVDSNGWVYIADRENH
                     RIQVFDSNGNFETQWVNLSRAAISTIDSGTGEELMYVGEYFAGIGSNDMGTDLGPRIS
                     VLNLKGEVQARIGRQSYGEQSGRFFSPHGIAVDSKGDIYVAEVSWSDYGSSMDPPREL
                     RSMQKLVKV"
     gene            complement(1709..3706)
                     /locus_tag="QF838_04755"
     CDS             complement(1709..3706)
                     /locus_tag="QF838_04755"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HJO60029.1"
                     /translation="MDNQTSFSIGDVIDPKDSTWAFDTHSNGPSGTLPFTEDILVNES
                     SGFHFGMSQNAGMGWNPAELLRKHFLILSTSGGLRDQDGSPIALGLHTGHFELALQVE
                     AAAREFKLAKTIPFSLMCSDPCDGRTQGTTGMMDSLPYRNDAAQVFRRQARSLPTAKG
                     ILGIASCDKGLPAMMMALAGLRDLPVVVVPGGSTLAVREGEDTGMIQSIGARFAQGEV
                     TLDYAQDVGCKACASPGGGCHFLGTAGTSQVIAESLGLAITHSALCPSGTTAWKNVAQ
                     RSARALLLMESENTLSSDIITDAALRNAMVVHAACGGSTNLLLHLPAIAHAAGRTRMT
                     VDDWNMVNKNTPRIVDVLPNGPVGFPTSELYTAGGVPEVMLHLRAMGLLDTNVKTVTG
                     QNLGENLDWWEDSERRHDVRKRLSEINSVNPDHVIMTPEDAKKRGLTSTVTFPMGNIA
                     PQGSVIKSTAIDPSVVDKDGIYRNTGPARVFSSENEAIRAIKSTDDKSKIKAGDIMVV
                     KCAGPQGTGMEELYQITAALKHLSYGKHVALITDARFSGVSTGACIGHVGPEALADGP
                     IAKLKDGDQIEIIIDRINLFGSINLVGELGEYLGSDWGDKELSKRPIPQDLSPHPKLP
                     EDSRLWAALQSASGGTWGGCVFDVDDIVKTLEAGQAALENK"
     gene            3794..4570
                     /locus_tag="QF838_04760"
     CDS             3794..4570
                     /locus_tag="QF838_04760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO60030.1"
                     /translation="METPKHIVGVQHGWNLMSANYQFESDISVDEVHYAPLSYGENSL
                     NLLGDVTGKHFVELGSGGSQNAIALAKLGAKVTAVDFSSSQLAFGVSESVKQKATIDF
                     LQADIQNLSYFQDNTFDSVISIFALEFIEYLDVFLSECNRVLKEGGQLILSTTHPLGA
                     FEWDADTNTLNVTDYFNPPVEIWKENGQRYSGVTYFRTVEELFTNVTDSGFKVEVLLE
                     PKPLGFDNEHLSPYKGDYWTEFRDRINLVPFAIVIKALKQ"
     gene            complement(4587..4790)
                     /gene="thiS"
                     /locus_tag="QF838_04765"
     CDS             complement(4587..4790)
                     /gene="thiS"
                     /locus_tag="QF838_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HJO60031.1"
                     /translation="MNINLNGKNHEINSGTSLIRLIDDLGLTGRKAIAIALNGEVLAR
                     DSYKETILNEGDSLEVVKAIGGG"
     gene            complement(4802..5428)
                     /locus_tag="QF838_04770"
     CDS             complement(4802..5428)
                     /locus_tag="QF838_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014622.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HJO60032.1"
                     /translation="MIDILKNPLLCLVTDRNRTSCRALESVVGQAVEGGTNMIQFRDR
                     DMEDVDRLHAAAKLREITEKKALFIVNGDVDLAKKVGADGIHFPENHIFDADTQELKE
                     DFILGQSVHSLEMATKAERLCIDYLIVGTLFPSKSHPHGRVSGTKIIEDISGKLPVPL
                     IGIGGISSQNCRKVIDSGASGVAVIGAISEADDPRLESQIILANISVS"
     gene            complement(5435..6211)
                     /locus_tag="QF838_04775"
     CDS             complement(5435..6211)
                     /locus_tag="QF838_04775"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HJO60033.1"
                     /translation="MEDPLVIAGKEFSSRLMVGTGRHRTMQEMIDSVRASGTQIITVA
                     IGRLNLQDINEKTILDYFDWDNYTILPNTAGSKTSEEAVLTARLAREVTGSSWIKLEV
                     IPDISHLLPDPIGTYDAARELVEDGFDVLPYIHADPVLAHRLAELGCATVMPLGSTIG
                     SGQGILTMEEIEMIISDVEVPVVVDAGLAVPSDASKALEAGADAVLVNTAIAQANDPV
                     AMGESFKLGVAAGRKAFLAGRIDRRKSAISSSPGAGVNSG"
     regulatory      complement(6289..6391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6523..7005
                     /locus_tag="QF838_04780"
     CDS             6523..7005
                     /locus_tag="QF838_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HJO60034.1"
                     /translation="MRHYTATGYVVWDQSVLLHWHKKINLWLPPGGHVEPNEDPVEAV
                     LREIFEETGLASKISKNSNTPTLTFDYPEIIEPPRHILIENIDDPDDGHHQHIDMIYY
                     CYPTDGIMDLLPGWSFFSYEVLKTALKSHQLEKTHLNPSIYPPLDVLALGVDAIDQTV
                     "
     gene            7093..8160
                     /locus_tag="QF838_04785"
     CDS             7093..8160
                     /locus_tag="QF838_04785"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HJO60035.1"
                     /translation="MVQNNIAVIRGDGIGIEVVEEGIKVLDAVSENYDFELSFNEYPW
                     SSKFYIDTGAMMPSDGLDILSNYDAIFLGAVGHPDIQDHITLNGLLLPIRRKFDQYVC
                     ERPSVLFDGIESPLSGKKPFDIDMVVIRENTEGEYANVGGFQYMDFPEEVGIQTGIFT
                     RKGCERVIRYSFELARRRNKKKHLTSITKSNAQGYGMLVWDRAFERISAEYPDISTDS
                     LLVDAAAMDFIRKPEFFDVVVASNLFGDILTDIAAIITGSMGLASSANLDPEGRYPSM
                     FEPVHGSAPDIMGKAIANPMAQILTGAMLLRHLGHDDAATDIEKAVLSVLSDGEILTP
                     DLYGSSTTSSVGDAIVSALGK"
     gene            8187..>10209
                     /gene="leuS"
                     /locus_tag="QF838_04790"
     CDS             8187..>10209
                     /gene="leuS"
                     /locus_tag="QF838_04790"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HJO60036.1"
                     /translation="MPIKTIDTRFPHESIETKWQNRWRDSQIYKVDDTDPRPKWYELT
                     MYPYPSGDLHVGHWYAMTGADIHARFRNMQGYNVLHPMGFDAFGLNAENAAIQRGIHP
                     HSWTMDNIKRMRQQLASMGPMYDWEREVVTCIPEYYKWNQWFFLQFYQSGLAYRDFAP
                     VNWCPSCVTVLANEQVIDGECERCDTTVTHRDMNQWFFRITAYAEELLDQSKMDWPDR
                     INTMQTNWIGKSEGVEFGFDISEYGLDSKTLVTFTTRIDTVYGVTFVVLAPEHPLVGA
                     LTAPEQEDSVKTYINDARKATEVERLAIDREKTGVFLGTYCVNPLTLDQIPILIGDYV
                     LNTYGTGIVMGVPAHDTRDFKFAQKYDLPIKVVVAPPEWDGKELDEAYVAAGTQVNSL
                     EFNGLDSNEGRLRIADHIESSNFGTKTVNYRMRDWLISRQRYWGTPIPIIYCSDCGVV
                     PVKEQDLPVLLPEDAEFLPTGESPLALHTEFVNAECPDCGKPGKRETDTMDTFVDSSW
                     YFLRYVSPKHSDAAFDEESAQLWNPVDQYTGGVEHAVMHLLYSRFFVKAARDLGLIKY
                     DEPFKRLFNQGTIIYKGAKMSKSRGNVIAPDDYVSVVGADTVRTYLMFIGPWEQGGEW
                     NDSGINGAARWLNKVWDISNLDPDIFTQNSNPAAERELNRLLHKTIMRVG"
BASE COUNT         2964 a         2261 c         2160 g         2824 t
ORIGIN      
        1 cgtgtcattg ggaccttcta tcatctgtac aggccagatg aaaccatgcc ctataactcc
       61 attatcctcg tcttcccaat tagtacgacg aatttctcca ccttctccca gttccctaga
      121 taatatttca gcttgctcaa gagcctgatc tgccataacg gtggcatagc ttaaataact
      181 tgaatcaaaa gaatcaattc tcgccagaaa atgctgaacc acaccaaccg gtgtttcgtc
      241 gccatcaaca tatgattcag taatttcttg aatgttttta taatgagtca aggggttggt
      301 cacattctta cccgcagtac cggcatcttg tgttttcact ggcaaatatt aattcggcct
      361 caacttcttc taatgcatag tttatcaaaa cgcgaagggc cttcgattca tccggcaacc
      421 caaatttaac cacttattcg gcaagccatt cggcttgatc agcttcaatc gtcacactca
      481 aatctgtctt ttcatgagcc ataatttcct ctctatttga atttgattta ttataactgg
      541 agatagcaaa ccgtttaagc ctacttataa acctccttta agggttgttt aacgtatgct
      601 cagaacagag atatggaggc atcagatgtc gaatggagat cagaattggg aatttggggt
      661 aaaggatcac ggtcattctg tcgttgacga gactgatatg gcaccggctg tgttgggaga
      721 agggaatttt tgttaccaag tttcagggca gaattggggt actctacctg acggttgggt
      781 atacaaagaa gctacagcag tggatgttga ctccaaagat agggtgtatg tcttcaatcg
      841 aggaactatg ccgatgatcg tattcgaccc agaaggaaac gttatcgata cgtgggggaa
      901 tggagtattt tcaaatccac acggcgtcac aatcgggccc ggtgatgaag tgtactgtgt
      961 agacaatgga gaccatactg tcaggaaatt tacatcagat ggaaaattac ttttaacagt
     1021 cggaactccc ggtaaatcag cagcgcctat gagtggcata ccgttcgcca gacccaccca
     1081 tgttggcgtc gagcccaata caggtaattt ctatgtagca gatggttata caaatgccag
     1141 tgttcataaa ttctcacctg acggtaagct attgttttca tggggtgaat ccggaactaa
     1201 tcctgggcag ttcaatattg tccacggggt caacgtagat tcaaatggct gggtctatat
     1261 agctgatagg gaaaatcacc gaatccaggt atttgatagc aatggaaatt tcgagaccca
     1321 gtgggtaaat ttgtcccgag ccgcgataag caccatcgat tctggcactg gtgaagagtt
     1381 gatgtacgtg ggtgaatatt ttgcaggtat cggatcaaac gacatgggca ccgacctagg
     1441 cccaagaata tcagttctta atttaaaggg cgaggttcaa gctcggatcg gaaggcaaag
     1501 ctatggtgaa caaagtggta ggtttttctc accacatggt attgcggttg actctaaagg
     1561 cgatatttat gtagctgaag tttcatggag tgattacggc agtagtatgg atccaccaag
     1621 agagttgcga tcaatgcaaa agcttgtcaa agtttgattc tatcctccgg gccctaaata
     1681 atcggacccg gatcgccccc gataaaaatt atttgttttc taaggcagct tgaccagctt
     1741 ccaaagtttt cacaatatcg tccacgtcaa aaacacatcc cccccaggta cctccagaag
     1801 ctgattgcaa agcagcccaa agtcttgaat cttccggtaa tttaggatgc ggtgacaaat
     1861 cctgtggaat aggtctttta gaaagctctt tatcccccca gtccgaccct aaatattcgc
     1921 ctaattcacc gacaagattt atacttccaa ataggttaat tcgatcaata ataatctcga
     1981 tttgatcacc atcttttaat ttggcaatcg ggccgtctgc caaagcttca ggccccacgt
     2041 gccctatgca ggcaccggta gacaccccgg aaaatctggc atcagttatc aatgcgacgt
     2101 gtttaccata ggaaagatgt tttaatgcag cggttatttg ataaagctct tccattcccg
     2161 tcccttgagg tcctgcacat ttcaccacca taatatctcc agccttgatt ttcgatttat
     2221 catcagtgct tttgattgcc cgtatggcct cattttccga agagaaaacc ctggctggtc
     2281 cagtatttcg gtatatcccg tctttatcta ccaccgatgg atctatcgcc gtacttttaa
     2341 ttactgagcc ctggggggcg atgttaccca ttggaaaagt aacggtactt gttaagcctc
     2401 ttttctttgc gtcctcaggg gtcatgatta catgatctgg atttacggaa ttgatttctg
     2461 acaacctttt ccgaacatca tgtctcctct cagaatcttc ccaccaatct agattttctc
     2521 ctaaattttg gcccgttact gttttcacat tagtgtccaa tagacccatg gcacgaagat
     2581 gcagcatcac ctctggaact ccacctgcag tatagagttc cgaagttgga aaccctactg
     2641 gtccatttgg aaggacatcg actattctag gagtattttt atttaccatg ttccaatcat
     2701 ctacagtcat cctagtccta cctgcagcat gagctattgc gggaagatgc aacaaaaggt
     2761 tagtagaccc tccgcaagca gcatgtacaa ccatggcatt cctaagggcg gcgtcagtga
     2821 taatgtcaga ggacaatgtg ttttcggatt ccatgagcag caatgctctt gcgctacgtt
     2881 gggccacatt tttccaagca gttgtacccg acgggcataa agcactgtgg gtaatagcta
     2941 gtcccaatga ttcagctata acttgtgatg ttcctgctgt accaagaaag tggcatcccc
     3001 ctccaggtga agcgcaagcc ttacagccaa cgtcctgagc atagtccaat gtcacttccc
     3061 cttgagcaaa ccttgcccca atggactgaa tcattccagt gtcttctcct tcacggacag
     3121 ccaaggttga accccccgga accacaacca ctggcaaatc cctaagtccc gccaacgcca
     3181 tcatcattgc tggaagccct ttgtcgcagg atgcaatccc taaaatacct tttgccgtcg
     3241 gcaaggatcg tgcctgtctc ctaaaaacct gtgccgcgtc attcctgtat ggaagtgaat
     3301 ccatcatccc ggtagtaccc tgcgtgcgtc cgtcacatgg atccgagcac atgagggaaa
     3361 atgggattgt cttggcgagt ttaaattccc gagcagcagc ttcaacctgc aaggctaatt
     3421 cgaagtggcc ggtgtgaagg ccaagggcaa tcggtgagcc atcttgatct cttaatccac
     3481 ctgaagtcga caaaattaaa aaatgttttc tcaacagttc ggccggattc cagcccatcc
     3541 cagcattttg gctcatacca aaatgaaacc cgcttgattc attaacaaga atatcttctg
     3601 taaacggtaa agttccagaa ggaccgtttg aatgagtatc gaaagcccag gtggaatcct
     3661 ttggatctat aacgtcgcca atactaaaag atgtctgatt gtccatttga acccctccgt
     3721 aagattagtt tgcgccaata gtgtaccaat tcagctatta ttcattaaat gcacaatgag
     3781 aggtgttaaa tagttggaaa ctccaaagca tatcgtcggt gttcaacacg gctggaattt
     3841 aatgtctgcc aattaccaat ttgaaagcga tatatcagtg gatgaggtgc attatgcccc
     3901 cttatcatat ggggaaaatt ccttgaattt attaggagat gtgactggta aacattttgt
     3961 ggagctaggt agtggtggtt cacaaaacgc gatagcttta gcaaaattgg gagcaaaagt
     4021 aactgccgtg gatttttcat ccagtcaatt agcgtttgga gtctctgaat ccgtaaaaca
     4081 aaaggcaaca atagattttc ttcaagccga tattcaaaat ctaagttatt tccaggataa
     4141 tacgtttgac agtgtgattt caatttttgc cctagaattt atagaatacc ttgatgtttt
     4201 tttgagtgaa tgcaacaggg ttctcaagga aggtggacaa ttaatattga gcacaacgca
     4261 tcctctgggc gctttcgaat gggatgctga cacaaataca ttaaacgtca ccgattactt
     4321 taatcctcct gtcgagatat ggaaggagaa tggtcaaagg tattccggcg taacttattt
     4381 cagaacagtg gaagagctgt ttaccaacgt taccgatagc ggatttaaag tggaggttct
     4441 cctggaacca aaaccgctag gatttgataa tgaacacctc agcccatata aaggtgacta
     4501 ttggactgag tttagggata ggataaattt ggtgcctttt gcgatcgtaa ttaaggcact
     4561 taaacaataa acaggattcc ccctaactaa cccccaccta tagccttcac aacttccaga
     4621 gaatccccct cattcaaaat ggtttcttta taactatccc gagcaagaac ctcgccattc
     4681 agagctattg caattgcttt ccttccagta agtcctaagt catcaatcag tcttatcaga
     4741 gatgtgccag aattaatttc gtggttttta ccatttaggt taatattcat tagatcaaac
     4801 tttaactcac acttatattt gctaaaatta tctgagattc caatctaggg tcatcagcct
     4861 cagagatggc tccaataact gcaacacccg aagctccaga atcaatcact tttcgacaat
     4921 tttgactaga tatcccacca atgcctatta atggaaccgg taatttccca cttatatctt
     4981 ctatgatttt agtcccacta actcgcccgt gaggatgcga tttagacgga aataaagtgc
     5041 caacaatcaa gtaatctata cataaccgtt cggcttttgt agccatttct aaactatgca
     5101 ctgattggcc gagtatgaaa tcctctttca actcttgagt atccgcatca aaaatatggt
     5161 tttcaggaaa atggatgcca tcagcgccaa ctttctttgc taagtcaaca tctccattta
     5221 caataaaaag ggctttcttc tcagttattt ccctcaattt ggcagcagca tgcaacctat
     5281 caacatcttc catatctcta tctcgaaact gaatcatgtt cgtcccacct tccactgcct
     5341 gaccaacaac agattctaaa gctctgcaag atgtgcgatt tctatcagtt acaagacata
     5401 acaatggatt tttcaggata tcgatcatca aatttcagcc agaattaacc cctgcaccag
     5461 ggctgcttga tattgctgat ttccttctat caattcggcc tgccagaaag gccttccggc
     5521 cagcggcaac tcctaatttg aaagattctc ccattgccac aggatcattg gcttgagcaa
     5581 tggcggtatt aactaaaact gcgtccgctc ctgcctctaa ggcctttgac gcatcagaag
     5641 ggacggccaa acccgcatct actaccacag gcacctccac gtcgctaata atcatttcaa
     5701 tttcttccat agtcaatata ccttgacctg aacctatagt tgatcccaat ggcattaccg
     5761 ttgcacagcc aagttcagct aatctgtggg ccagcaccgg atctgcatga atataaggca
     5821 gaacatcaaa gccatcttct accagttctc tagcagcatc gtaagtccca attgggtctg
     5881 gtaacaaatg acttatgtca ggaataactt caagcttaat ccaggaagac cctgtcactt
     5941 ctcgggctaa gcgagcggtc aatacggctt cttctgaagt tttactaccg gcagtattgg
     6001 gtaatattgt atagttatcc cagtcgaaat aatccaaaat ggttttttca ttaatatctt
     6061 gcaaattgag cctacctatg gccacagtta ttatctgtgt accagaagct cttactgaat
     6121 ctatcatttc ctgcatggtt cggtggcgcc cagtcccaac catcaatcta gagctaaatt
     6181 ccttacctgc tataactaaa ggatcttcca tttcatatct ccttggccat ctaaattatt
     6241 ggccatctaa attaaccaga caaaaaaaat cacccctgga gggtgatcgg agattagatc
     6301 cataactccc tccgctggca ttacccagtt caggtcttgg gtcggcattt aaataccctc
     6361 tcagccattt gaggctcccc tatatacgcg tatttcgctc ggcaagaata tatgcaagat
     6421 acgtgttaaa gtcaatgttg gaagctgtat gcctttcatc acaagcaatt ttggcctcaa
     6481 aaacaaaggg attgacctta aggagataat tcattgtctt ttatgcgtca ctacaccgct
     6541 actggatatg tagtatggga ccagagtgtt ctactacatt ggcataaaaa aataaattta
     6601 tggctgccac ctggaggcca tgtggagccg aatgaagatc cggtggaagc ggtattacga
     6661 gaaatttttg aagaaacagg actggcgtca aaaatatcta aaaattccaa tacacccact
     6721 ttgactttcg attacccaga aattattgaa cctccgagac atatactaat cgaaaatatt
     6781 gacgatccgg atgatgggca tcatcaacat atagacatga tttattattg ctaccccacc
     6841 gacgggataa tggacttact accaggttgg tctttctttt catatgaagt actcaagaca
     6901 gccctcaaat ctcatcaact tgaaaaaacc cacctaaacc cctcaatata ccctcctcta
     6961 gatgtactag cattgggggt agatgctatt gaccagactg tatgaatctt tactgggtat
     7021 aaaaaaactg atcttttcag gtattatttt acggctccac aaatcattag tgacaaggtg
     7081 taattaaata aaatggttca gaataatatt gctgtaatcc ggggagatgg aataggcatt
     7141 gaagtagtag aggaaggaat aaaggtttta gacgcggttt cagaaaatta tgattttgaa
     7201 ctttccttca atgaataccc ttggagttct aaattttata tcgatactgg ggccatgatg
     7261 ccttcagatg gtctcgatat tttgtctaat tacgacgcaa tctttttagg ggctgtcggt
     7321 caccccgata ttcaagacca tataacctta aacgggttgt tgctacccat ccgaaggaaa
     7381 tttgaccaat atgtgtgtga acgacccagt gttctatttg acggtattga atccccactg
     7441 tcagggaaaa aaccgttcga tatagatatg gtagttatcc gggaaaatac agaaggggaa
     7501 tatgcaaatg tcggaggatt ccagtatatg gattttccgg aagaagtagg tattcaaaca
     7561 gggattttta caagaaaagg ttgtgagagg gtaatccgat attcctttga attggctcgc
     7621 agaagaaata aaaagaaaca cctaacctct atcaccaaat ctaatgcaca aggatatgga
     7681 atgttggtat gggatagagc ttttgagaga atatctgccg aatatccgga tatatctaca
     7741 gattccttat tggtggacgc tgcagcaatg gatttcatta ggaaaccgga attttttgat
     7801 gtggttgttg ccagcaatct ttttggcgac atattgaccg acattgccgc aataattact
     7861 ggaagtatgg gactagcatc gagtgctaat ttagatccag aaggaagata tccctcaatg
     7921 ttcgaaccgg tacacggcag tgcgccagat ataatgggca aagccattgc taacccgatg
     7981 gcccaaattc tgaccggggc tatgcttctc agacatctag gtcatgacga tgctgcaacc
     8041 gatatagaaa aggcggtgtt atcagtcctt tcagacgggg aaattctaac cccagatttg
     8101 tatggttctt cgacgacatc ttctgttgga gatgccatag tatctgccct agggaaatag
     8161 ttgaaaaatc aagaagagat tattaaatgc caatcaaaac aatagatacc cgtttccccc
     8221 acgaaagtat agagactaaa tggcaaaatc gatggcgtga ttcccaaatt tataaagtcg
     8281 acgacaccga tcctagacct aagtggtatg aattaacaat gtacccatat ccctcgggcg
     8341 acctacatgt cggtcattgg tatgcaatga ctggtgcaga catacacgca agatttagaa
     8401 atatgcaagg ttacaacgtt ctccatccaa tggggttcga cgcatttggt ctgaatgcag
     8461 aaaacgcagc tatacaacga ggtatacatc cgcattcgtg gacaatggac aacattaaaa
     8521 gaatgcgcca acaactagca tctatgggtc cgatgtatga ctgggaaagg gaggtagtta
     8581 catgcatacc ggaatattac aaatggaacc aatggttttt cctgcagttc tatcaaagtg
     8641 ggttagctta tcgtgatttt gcccctgtca actggtgtcc ctcatgcgta acggttttag
     8701 caaatgagca ggtcatagat ggggaatgtg agaggtgtga caccaccgtg actcataggg
     8761 atatgaacca gtggttcttc cgcatcacgg cctacgctga ggaattattg gatcaatcca
     8821 aaatggactg gccagaccgc ataaacacga tgcaaacaaa ttggattgga aaaagtgaag
     8881 gtgtggaatt cggttttgat atctcggaat acggtctgga ctcaaaaact ttagtcactt
     8941 tcactacccg aattgatact gtttacggtg tcacctttgt ggtgttggca ccagaacacc
     9001 cattggtagg ggctttaact gccccggagc aagaggattc tgtaaagacc tatataaatg
     9061 atgcgagaaa agccacggaa gtggaacgac tagcaatcga tagggaaaaa acaggagtat
     9121 ttttgggaac ctactgtgtc aatcctctta ctctggacca aataccgata ctaatcggcg
     9181 actatgtcct aaacacatac gggactggta ttgtaatggg agttcccgcg cacgatacga
     9241 gagattttaa atttgcccaa aaatatgacc taccaattaa agtggttgtc gcccctcccg
     9301 aatgggatgg caaagaatta gatgaggcct acgtagcggc agggactcaa gtaaattcac
     9361 tggaatttaa cggtttggac agtaacgaag gccggctgag gatagcagat catattgaga
     9421 gtagtaactt cggtactaaa actgttaact ataggatgag agactggctc atttcaagac
     9481 aaagatattg gggcacacca atacccataa tttactgtag tgattgtggt gtcgtccctg
     9541 tcaaagaaca ggatcttcct gtgttactcc cggaagatgc agaatttcta ccaacaggtg
     9601 agtcaccctt ggctctacat accgaattcg ttaatgctga gtgtcctgat tgtggtaaac
     9661 ctggcaaaag agaaacggac acgatggaca cctttgtgga ctcatcatgg tattttctta
     9721 gatacgtcag ccccaagcat tcggacgccg catttgatga agaatcggct caactctgga
     9781 acccggttga tcaatacacc ggtggagttg aacatgcagt aatgcacttg ctatattcaa
     9841 ggttcttcgt caaggctgca cgcgaccttg gtttaatcaa atacgatgaa cccttcaaaa
     9901 gattgttcaa ccagggaaca ataatttaca aaggggccaa aatgagcaaa tctcgtggga
     9961 atgtgatcgc cccggatgat tatgtctctg tagttggggc ggatacagtt agaacttatc
    10021 taatgttcat tggaccctgg gagcaaggtg gagaatggaa tgattcaggg ataaatggag
    10081 ccgctcgttg gctcaataaa gtctgggaca tatccaattt agatcccgat atttttacac
    10141 aaaattcaaa tcctgccgca gaaagagaac taaacaggct tttacataaa actattatga
    10201 gggtggggg
//
LOCUS       DASCNS010000136        23962 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001130858, whole genome shotgun sequence.
ACCESSION   DASCNS010000136 DASCNS010000000
VERSION     DASCNS010000136.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 23962)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23962)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23962
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001130858"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="QF838_04795"
     CDS             complement(<1..1987)
                     /locus_tag="QF838_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HJO60037.1"
                     /translation="MFEAVDSKMNFPEMETGILKWWNENQIPDKYMAKNEDSDKRYSF
                     IDGPITANNPMGVHHAWGRSYKDLFSRFRTMQGYRQRYQNGFDGQGLWIEVEVEKELG
                     FTSKTDIEGYGIDKFVTLCKERVQRFADVISEQSKQLAYWMHWDNSYHTMSDENNYTI
                     WHFLKTCHEKGLLYEGTDVMPWCPRCSTGLSEHEIVTEGYVEIEHPGLFVKFPILDSD
                     SETTEESLLIWTTTPWTLSSNCAAAVNKDIDYVKVKQDHGYLYLAKSRISSVAGEYEV
                     VSELKGSELVGLNYEGPFDELPAQEGVKHRVVAWDEVSETEGTGIVHIAPGAGKEDFA
                     LGKREGIPTIAPLDDLGDFVEGFDWLTGLNVYEVNDKIYESLKSKGKFYRLERYKHRY
                     PHCWRCGSELVFRLVDEWFIKMDPLRGPLSRVTQNIRWVPEFGMKRELDWLRNMDDWM
                     ISKKRYYGLALPIYKCSCGNFDVIGSEKELEERAVEGWDEFEGNSPHKPWIDSVKINC
                     SSCGEKLSRVSDVGNPWLDAGIVSFSTLNYRHDRNYWKDWFPADWISESFPGQYRNWF
                     YSLLTMSTVLADSEPCKNIFSYALMRDANGEEMHKSKGNAIWFEDAAEKMGVDAMRWQ
                     FAGQNPASNLNFGFGSADEVGRQFLIPLWNVYSFFVTY"
     misc_binding    complement(2019..2255)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2303..3028)
                     /locus_tag="QF838_04800"
     CDS             complement(2303..3028)
                     /locus_tag="QF838_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HJO60038.1"
                     /translation="MTEITPIADETLEVLKKIPTQTLIDGLWVKGWPSAYVEGAKPLQ
                     LGQSMAGRAVTLRFVPHRPDIAQDKPKKEQSAEYVAIELCGPSEVLVIDAMGWQYSSI
                     GGDIKFMRLMQLQVGGLVTDGGVRDSVALKEYGFPVYSAATTAKQGPADFWPWQVNDA
                     IQCGGVLVRPGDAIVGDDDGVVVVPRSEVEEVIRIAHEREEVEEVVKAQLEIENCSPG
                     KYYPFNENTWKLYEEKTGRKRVQ"
     gene            3130..3570
                     /locus_tag="QF838_04805"
     CDS             3130..3570
                     /locus_tag="QF838_04805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJO60039.1"
                     /translation="METLNTDILVIGGGVNGTSIAMALVARSAGKITLIEKGGLATGA
                     TGRSGAMVREHYLNVELVRMASDAKNFFEEWSKEMGYDLKFQTTGRILLFDESDAGAA
                     IANGEMNRAEGVNIETLSPEKTMGLIPLANLEDVSISLYEPEAG"
     gene            3627..4316
                     /locus_tag="QF838_04810"
                     /pseudo
     CDS             3627..4316
                     /locus_tag="QF838_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            4394..4481
                     /locus_tag="QF838_04815"
     tRNA            4394..4481
                     /locus_tag="QF838_04815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4428..4430,aa:Leu,seq:gag)
     gene            4497..4573
                     /locus_tag="QF838_04820"
     tRNA            4497..4573
                     /locus_tag="QF838_04820"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4531..4533,aa:Cys,seq:gca)
     gene            4653..5522
                     /locus_tag="QF838_04825"
     CDS             4653..5522
                     /locus_tag="QF838_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024118.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO60040.1"
                     /translation="MPLKESSELVLLGTGGGPKIWAARSQPASAILVNGDVYIIDAGD
                     GITGQLAKSGIDTNSIKAVFITHNHSDHVADYGTLLLRAWQSGHKGPIDCFGPPPLKD
                     MTNSYMAYMNWDINLRIQHEARPDFKSMINVLEITHNSSVYQDSNVKVDCIKVPHGEA
                     DPSYSYRFQIDGKVIVFSGDTSKSEALVSFASEADILVHEVLNLEGVEAIIEKTYPGN
                     EAFRSHIIEGHTTMSEVGEIASQAKVKILVLNHFVPTGSPYLDKEEIWQDGVRKTFNG
                     QIIVGTDLLRIPL"
     gene            complement(5543..6583)
                     /locus_tag="QF838_04830"
     CDS             complement(5543..6583)
                     /locus_tag="QF838_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60041.1"
                     /translation="MRFGSWVFPISQNSKNDGHVIDDTLTEIDRWEALGFDSIWLSEH
                     HFDGATAYADPVVFAGAIAQRTKNVTIGFAVVEMALHHPVRLAAQVALLDHLSKGRMI
                     LGTGKGSAFNEYEYIGFGVPMSDAADSISEAEEIILEAWSGKPVQFKGKYWDLAFPEL
                     RPMPLQKPHPPLLRACISRESTIEMGFKRRPILIAAQDNHSIKDRLQVFTKSLESTEI
                     TESGMEDILDQVWVNKNIVVAESRSEAEEIAFDGYTREQTFFKVARDKYNPKSGSPGL
                     SKPREISKKDFDNIFITGTPRDVAEQISELDKIGVKNLMMKINTGEMDRSIVFRTMDL
                     LAEHVKPLFLTG"
     gene            complement(6668..6823)
                     /locus_tag="QF838_04835"
     CDS             complement(6668..6823)
                     /locus_tag="QF838_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60042.1"
                     /translation="MANLGRWWSSYSYGGILDFIAIGPDDLYTSKEIEVMLKKYILKK
                     SAADSSY"
     gene            complement(6896..7753)
                     /locus_tag="QF838_04840"
     CDS             complement(6896..7753)
                     /locus_tag="QF838_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025008.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HJO60043.1"
                     /translation="MNEAFFNRDSDNKNVYHPTLYARGPWDPKTLHGRVLSGLVAFEV
                     ETNCLSEDEVDTFQASRITVDLFRPPPMSPLIVTNQIVRSGRRIKVVDVNIASDIKGR
                     GIIDIARGSVVMLKKSENPDGEIWSPTAWEIRSPPEDMPPPYVAQKGPDRTASLPIWQ
                     TVNVQDDTGEPYDEYRSQNRKLGVRRAWIRETYSLISGMLPSQLVRIAQVADFANPFT
                     NSGTKGLSYINADITLYLQRTPIGSWIGTETSYHGADQGVSVGTMSLYDRSGRVGTST
                     VCGLAQARS"
     gene            complement(7758..8744)
                     /locus_tag="QF838_04845"
     CDS             complement(7758..8744)
                     /locus_tag="QF838_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO60044.1"
                     /translation="MKINRFGDTELQVSQLGFGCGAVGGLMVLGDHKEMMDAVEYAID
                     SGINYFDTARMYGDGLSEIHTGAILRELDMTDLLIGTKVRIASDEFENISKTISDQID
                     NSLLRLGINTVDIVYTHNTIGAERNISSGTLSVDDLNLIVEVFEKAVASGKLRYWGFN
                     GLGETDSIKEALVKYSPSGIHTCYNMLNPTSGYHLEKTFPYQNYEELMKLASTEGIGT
                     VGIRILAGGALSGSLKRHPIAQQNVAPIATSSTFQEDVTRTSAFEFLLKEGFVDNLVE
                     ASIRFAISHDNLDTALIGLSNFDQLKQAIDYANQGPLPQEALDKICEIWTVS"
     gene            complement(8850..9632)
                     /locus_tag="QF838_04850"
     CDS             complement(8850..9632)
                     /locus_tag="QF838_04850"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO60045.1"
                     /translation="MDFEGKVALVTGGGRGIGKAAATKFAEGGADIVIADIDPQTAKD
                     TAEEISALGVRAFSISLDIGDIGQIENMVHEAVSKLGSVDILFNNAGLTRHISFFDIE
                     EDHWDQINRVNAKGAFFCMQKVANQMVEQARGGRIINTASIAGKGYRRASNAAYAASK
                     GAVIAMTHSAASVMAEHDVNVNAICPGIVQTDMLANILSLRSTELGASSEEVKAMMTE
                     EIPLGRPNDAEDVAALAVFLAGPGSRNITGQAFNVDGGMIMH"
     gene            complement(9648..10415)
                     /locus_tag="QF838_04855"
     CDS             complement(9648..10415)
                     /locus_tag="QF838_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60046.1"
                     /translation="MPLDAFNLSGKVALVTGGNSGIGLGMAKGLAESGANVCIWGTNI
                     EKNEKSQKELSSLGIKAHAIRCDVSSEDQVETAFQETLKVFGKVDSCFANAGAGGGAP
                     FHEFPTDLWRKVMGINLDGTFFTFRAASKHMIERGEGGRLVGTSSTSAIHGAARNEAY
                     ASTKGAMLAMVRGLSVEMARYAITANSIIPGWIETAMTSARVGDERFEDRVMKRVPAR
                     RWGTPEDFAGIAVYLASDASSYQTGEEFVIDGGYTRF"
     gene            complement(10546..11109)
                     /locus_tag="QF838_04860"
     CDS             complement(10546..11109)
                     /locus_tag="QF838_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012383.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HJO60047.1"
                     /translation="MDQNTDFELDGLEILYSKHEVQSAVASLAQSMVEYYDNLSTINV
                     VPVMTGGMQFSAYLLSELEKKRPGKWLVSPIFASAYSSDEVLTEPTIEFPTTFDNRVD
                     LQAPTVVVDDLLDTGTTMMKLMKDIQSRGIQKVDLAVLIDRKRNQPKEIVPRFCGFQL
                     KSDRWLVGFGMDSEQRYRGLEAVYVQI"
     gene            complement(11171..12115)
                     /locus_tag="QF838_04865"
     CDS             complement(11171..12115)
                     /locus_tag="QF838_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60048.1"
                     /translation="MNPETLAIVISYATPLVFAVLGETITEKSGVVNLSLDGSMLLSA
                     MTGFAVSFTTGNVVLGLIAGGLVSSNLALIVAFSSIRLRLNQIAVGIVLTLLSTKLAA
                     FLGQDFVRKPGPSVQDLHIPVLKDIPGVGPVLFQQNLLVYFSYILIGLSFWFIFHTRQ
                     GLNLQAAGERSEAAYARGIDVNKLRYFYTALGGFLVGLGGAAFSLDVKLGWSDGHIIN
                     FGWIALAIVIFGGWHPIRAALGCYLFGVLQLVALKLQPVLPQLSQVLPIIPFPLMIMT
                     LLFINHKKIQYLLLKNSMFGRFITARPPSALGESFQRL"
     gene            complement(12119..13162)
                     /locus_tag="QF838_04870"
     CDS             complement(12119..13162)
                     /locus_tag="QF838_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60049.1"
                     /translation="MKGVTGPIYYYSTAVLVSMVFVSILLTLFKIPILETYTLLITGS
                     LGSMTKLGDTLMVSVPLIIASASLVITFRAGLWNIGVEGQIIAGAVAASFIAREIHMP
                     PGFLLPAMIVAGFLGGGFWGLLVGLLKVKGRVNEIFGGLGLDFVATGLVVYLVIGPWS
                     RKGIASTSGTEPFQGSAWFPTIEGLRLSPLAVSIALLVVLTVLFVFNRTQFGLRLRAI
                     GLNPDSSYRFGISTSKYILMAFILGGAVAGLAGVTQVASVHHRLIPSISGGYGFLGIL
                     VVLLAGYRPSLVIPIAFFFSMIAIGSIQWQLRLGLHSSLGGVFQGTLVLSVILISAMR
                     LLRQRRELHAKAE"
     gene            complement(13159..14622)
                     /locus_tag="QF838_04875"
     CDS             complement(13159..14622)
                     /locus_tag="QF838_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJO60050.1"
                     /translation="MSLKLLNISKSFGTVLANNDISIDIGQGEIHGLLGENGAGKSTL
                     VNIISGSLTSDAGTVELDGEYLDLSSPTDAIEAGIGILHQEPLVFLPFSIVDNFLAGL
                     PKSRDFDRKLVRDDIHKISQEYGFDLDPDTVASSLTIGERQQLEMARLLWMGARVLIL
                     DEPTTAISEAQRENLFKTIERLAASGMSVIFVSHKLDEIQSICDRVTVLRKGVLIDTL
                     TVPCPTSDLVNLMFGGYTQSLPQNQTVLGQDLLILDGICAKEETSTIEEVHCKFSEGE
                     IIGLAGLEGAGQRLLLRNITGLTKTYRGSTYFKGENITNHSYKWYMENGFHYLPADRA
                     GEGLIQGLTITEHFALTGNQSRKTIDWKESRQIAECKLQEYSIRGTVTSKPEDLSGGN
                     QQRLLLALMPDNLSVLSMEHPTRGLDIESAEWIWQELLDRSKSGTTIIFSSSDINELI
                     NFSDRILVCFSGKIIGNLSSKETTPYEIGSLIGGVQS"
     gene            complement(14643..15839)
                     /locus_tag="QF838_04880"
     CDS             complement(14643..15839)
                     /locus_tag="QF838_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HJO60051.1"
                     /translation="MRFKLLPLILILLLSLGACSSSGGVDSDSFKLGLILVGPQNDHG
                     WSQAHFEGASYAVSKTGGEVITIDKVNPADSPNVTVPQIVEDMISQGANLILATSDDM
                     KDGILEAAAAHPDTPMIWASGDTAWSDGEDYRSDLKSLGNVMGKMEYGKMIAGCAAAL
                     QSDTGNISYLGPLINAETRRLVSSTYLGAKHCWETVKGKSASDLKFKVTWIGFWFNIP
                     GVTQDPTVVVNDFIASGSDVVISGIDTTEAIVVAGQAAASGKTVWAVPYDYEGACLEA
                     PAVCLGVPYFNWGPSYHKIAKSVIDGNFKTSFDWVGPDWDNINDHDSSAIGFMKGEGL
                     TADNSKSLDEFIQKLAKDELNLWRGPINYQDGSAFLADGKEATDKEIWYLPMLLEGIE
                     GASTTE"
     gene            complement(16000..17052)
                     /locus_tag="QF838_04885"
     CDS             complement(16000..17052)
                     /locus_tag="QF838_04885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60052.1"
                     /translation="MKFGVFWQVPGYEGSDVGRRHWETIEELILAENLGFVEGWLAEA
                     PFYPTRPMSEPLLVVAAAAQHTTKIRFGTLATQVPMHHPLNLATEVATCDILTKGRLD
                     LCIGGRWGGPSSQVMGVSSEIDGDESRAMVSEYSDVLKNAWAESRLNFKGNYWDFNDV
                     PVLPKPVQGPYPPLLMAANSDGSFKFAAQNGLGVVGITLSQSIANLGAHIKTFKANVN
                     EGAANGNPQPFHVVVSFFVAESKEKAHDLMRKNWLDSDVIGDGPPVHSSAIGGGRHDF
                     SSGAGGWGSWNFATALEHCIYDSPEGCIESLRELEDRIPGMDQCILEFNRRGRNTTEV
                     VQHSMKLFAEKVMPYL"
     gene            complement(17072..17386)
                     /locus_tag="QF838_04890"
     CDS             complement(17072..17386)
                     /locus_tag="QF838_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60053.1"
                     /translation="MFYRIFTGDDGQSHMEEYSPPADLPATGIVFRWHEPGNFIDWHV
                     APKKQFIVTLEGQVEIGLGDGSVYTLGPGDMMLAEDLTGQGHTTKAVGDRTRVSIAIP
                     VE"
     gene            complement(17505..17765)
                     /locus_tag="QF838_04895"
     CDS             complement(17505..17765)
                     /locus_tag="QF838_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60054.1"
                     /translation="MSSGSKGFPKMGKPQETVDMTNLSIPEQIARVDEAINEAQYGVG
                     TTFLTSSHMVGTYALPKSLEKKIKCKISPSSTSGEWKLDIRP"
     gene            complement(17737..18981)
                     /locus_tag="QF838_04900"
     CDS             complement(17737..18981)
                     /locus_tag="QF838_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HJO60055.1"
                     /translation="MQIATASRKGKIDKNTFWALVVSGVLLLILGYTIYAGTHGEAVF
                     WLAGIAFGITVQRSRFCFTSAFRDFFLLGQTRILKGVLVGLAVCTVGFVMVMSTVSPN
                     PGFGVPPSDANVLPVGLSTLIAGTLFGIGMVLAGGCVSGSLYRMGEGYVGSWLAIGGV
                     MVGLFLLNNSWNWWWDNLISSEAQIWMPAEISYTGALVVTTFLLAALLYLAVWRERRA
                     TAGFIMPEIKRKSQENNPANTTSEITGYLRNIFRKEWSPIIGGIALGIINILLFVRFR
                     PLGVVGEISRWTTSLSTNLGLQELHLKGIEGLGACAMTVAEGSWLTGGFFLNFGIVAG
                     ALASAVFAREFKLRIPQTPVRYVQSIVGGVIMGYGAGLGLGCTLGAFFSAVPSLALNG
                     WVYAVGLALGAYLGVQWIKRFP"
     gene            19397..19639
                     /locus_tag="QF838_04905"
     CDS             19397..19639
                     /locus_tag="QF838_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013379.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HJO60056.1"
                     /translation="MPNDSNPILLDVRGEICPYPMLRTNKELDNSKQPLTALDVLTDH
                     PPALSTIPPEAMKRGYGCEIEEMEAGEWKIRLFKPE"
     gene            19676..20620
                     /locus_tag="QF838_04910"
     CDS             19676..20620
                     /locus_tag="QF838_04910"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HJO60057.1"
                     /translation="MLIHRLTKMGFGMLVGALILGVLSACSSTSGVSEDTSLDSRGYS
                     STERLVSTKWVADNLDNKNVKVIDIRKAEDYDAGHIPGALNYSSKELQVESGGVKGLI
                     PPSTDISNKLSSLGIKSDDTIIIYDGIKNLWSTRFLWTLDVYGHDDARMMDGSFGLWE
                     KENRKITADATPAKTSTYKFTGTANSELVTSIETVLGSLDDSSSVVLDTRGAEEFAGR
                     DVRASRGGHIPGSIHVEWVNNVDEDGRFLPASDLKELYASAYITDELGDIYTLCQTAV
                     RATHSWYVLADLLGFDNVSVYDGSWTEWGNREDTPIDS"
     gene            20749..21537
                     /locus_tag="QF838_04915"
     CDS             20749..21537
                     /locus_tag="QF838_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60058.1"
                     /translation="MGTLDGRVALVTGSSRGIGEYMALHFAKAGAKVAIAARTTEVTD
                     KRLPGTIYSVAESINKDGGEAIGVQMNMRDPESITAGVDETVEKLGGLDIVVNNAAIL
                     VPGSLETVQDRHIDLIWQIDLRGPLILMKRALPHLKSAGAGDFINVSSVAGVFPGPGP
                     YEDTPSRRDGSFYGMIKAGLERFSQGLAKELQGDKIKVNVLSPQGRIKTPGNIWAEND
                     PENPSLEFEAADEMGKAGVWICEQPNTYTGHILFDQDVCKEQNL"
     gene            21437..22291
                     /locus_tag="QF838_04920"
     CDS             21437..22291
                     /locus_tag="QF838_04920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HJO60059.1"
                     /translation="MKWAKRVYGYANNLTPILAISFLIKMFVKSKICSKETDSHSNIT
                     MPLQHLRLLHTSDTHLGDHLGHPSSNNALQKVVSETILLGADFLLLAGDVFDNERISD
                     ETVKFFLDEIYKLETPVILLPGNHDLMHDTSIYKRGIFKDYPSNLFIFKEEDGEIIEF
                     YDQGIKFWGKAMSEHSPSFQPLSPLPHPEKNTWFIGMAHGHFEAIETNSGRSSLISPG
                     EINKSPFNYIALGHWDLYTDVSQGQNLAVYSGCPMTIGAKSGPGFVSLVDLDPEKGTL
                     YEKWAIKS"
     gene            complement(22288..23082)
                     /locus_tag="QF838_04925"
     CDS             complement(22288..23082)
                     /locus_tag="QF838_04925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60060.1"
                     /translation="MTDMRLEGKKVIVTGGMSGIGLAIVSRFIKEGAIVLVADVKEDS
                     NGIIPSLASSSGGGVHFCKTDVSDMAEVKKMVEYAVEVMGGVDSVVNNAASFTFGGAD
                     VLDYQEWMKAILVNVIGTANVCKSTLKYLKKSDHPTIVNIASISSFIAQAKSLPYNST
                     KGAIAQLTRCLAMDWGEYGIRVNGICPGDIHTEGWYQRGISRQKKIDPADPEGFIQEA
                     SSMSLMDRVGKPEEVANLALFLASAEASYITGAMVVIDGGATVGMN"
     gene            complement(23100..23786)
                     /locus_tag="QF838_04930"
     CDS             complement(23100..23786)
                     /locus_tag="QF838_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60061.1"
                     /translation="MKVILAGIEYVGSTTISRMLSDWKLKNMGEPFFMGLVHDHSKLP
                     HTSGHPDDTTLEEQEQILNLSPKLKEMYHRYGMYYHIHHYVQEDDLSVGFHIEEAVYA
                     RRYYGYGLDGEQFDRNNVFVGIENRIKQITTDPIITVHMVASPDIIESRMLALKDHPE
                     HTNSPLQVEDIEDVMADYQYFISKSDIGPVVKVDTSIDSPEDTLEEVVTLLDPYFTKT
                     DLERIENHKG"
BASE COUNT         7030 a         5736 c         4445 g         6751 t
ORIGIN      
        1 cgtatgtaac gaaaaacgaa tatacattcc ataaaggaat taaaaattgc ctacctacct
       61 catcagcact accaaaccca aaattcagat ttgaagcagg attttgaccg gcgaattgcc
      121 aacgcatcgc atcaaccccc attttttcag cggcatcctc aaaccagata gcattgccct
      181 tacttttatg catttcttcg ccatttgcgt cccgcattaa cgcatagcta aaaatattct
      241 tgcaaggttc agaatcggct agaactgtac tcatcgtaag tagggaatag aaccagtttc
      301 tgtattgccc aggaaaactc tcagaaatcc aatcagctgg aaaccaatct ttccaataat
      361 ttctgtcgtg tcggtaattt aaagtcgaga aagaaacaat accggcgtca agccatggat
      421 ttcctacgtc tgaaacacga gacaatttct ccccacaaga agagcaatta atcttcaccg
      481 aatctatcca aggtttatga ggtgaattac cttcaaattc atcccagccc tcgacagccc
      541 tctcttccaa ttctttctca cttccaatta cgtcgaaatt accgcatgaa catttataaa
      601 ttggaagggc taaaccatag tatcttttct tagaaatcat ccaatcgtcc atatttctaa
      661 gccagtccaa ttcacgtttc atcccgaatt cgggaaccca ccgaatattt tgagtaactc
      721 gagatagggg ccctcttagt gggtccattt ttatgaacca ttcatccacc aatctaaaaa
      781 ccaactctga accacatctc cagcaatgag gatatctgtg cttgtaccgt tctaggcgat
      841 aaaacttacc tttgctcttt aaactctcat atattttgtc attgacttca tatacattta
      901 aaccggtgag ccaatcgaac ccttccacaa aatctcctag atcatccagc ggtgctatgg
      961 ttggtatacc ttccctttta cccaatgcaa agtcctcttt accagcaccc ggagcaatat
     1021 gaacaattcc agtaccttct gtttcggaaa cttcatccca tgctacaact ctgtgtttca
     1081 ctccttcctg agcaggcaat tcatcaaatg gaccctcata attcagtcct actaattcag
     1141 aacctttgag ttcagagacc acttcatatt ctcctgccac tgaggatatt cgagatttag
     1201 ccaaatacaa atagccatga tcttgcttaa ctttcacata atcgatgtct ttatttacag
     1261 cggcagcaca atttgaagat aaggtccaag gagtcgtggt ccatataagg agagactcct
     1321 ctgtagtttc cgagtctgaa tcaagtatcg gaaatttaac aaacaagccg gggtgttcta
     1381 tttctacata cccctctgta acaatttcgt gttcggacaa ccctgtgctg catcgagggc
     1441 accatggcat tacatcagtg ccttcataca ataaaccttt ctcatgacaa gttttcaaga
     1501 agtgccaaat tgtgtaattg ttctcatccg acatagtgtg ataggaatta tcccagtgca
     1561 tccaataggc cagttgcttt gattgctctg atataacatc tgcaaatcgc tgaaccctct
     1621 ccttacacaa cgtcacaaat ttatctattc cgtatccttc tatatcagtt ttggaggtaa
     1681 aacctaattc cttctccact tccacttcta tccacaaccc ttgcccatca aacccgtttt
     1741 ggtacctttg cctatatcct tgcatagtcc taaatctgct aaaaagatcc ttgtaactcc
     1801 taccccaagc atgatgcact cccataggat tgttagcagt tatcggccca tctatgaagg
     1861 aatatcgttt atctgaatct tcattttttg ccatgtattt gtctggtatt tgattttcgt
     1921 tccaccactt caaaataccg gtctccatct caggaaaatt cattttcgaa tctactgctt
     1981 caaacatatt tactcccaaa atataaggcc cgccccaatc aagggacggg ccttaaaata
     2041 gtccgcggta ccaccctaat tccccgtatc agacgaggct cttagttaaa aagcaccgat
     2101 aacgtagtgc agatccgtca tttcctaatc acttataagt tttcagaatg aaactccgga
     2161 gtgatcttca agctagccgc aagtaccctc tctcagcaac caagggctct ctgaaccgca
     2221 atctagtctc tactcgtctc catcaaagtt atttatttag ctgcaacaag agtagaaaag
     2281 agcgtgcctt gtgtcaagat tttcattgaa ctctctttcg gcccgttttc tcttcataga
     2341 gtttccaagt attctcattg aacgggtaat acttaccggg agaacaattc tcaatctcca
     2401 attgggcttt aactacctcc tcaacttcct ctctttcatg tgctattctg attacctctt
     2461 ctacctcaga tctgggaaca acaaccacac catcgtcatc ccctactatc gcatcgccag
     2521 gtctaaccaa tactccacca cattgaatag catcgttaac ctgccagggc caaaaatcgg
     2581 ctggtccttg cttggcagtt gtagcagccg agtaaaccgg aaacccatac tccttcaaag
     2641 cgacactatc tctcactccg ccatccgtaa ctaagccacc gacttgcagc tgcatcagac
     2701 gcatgaattt aatatctcct cctatggagc tgtactgcca gcccattgca tctattacca
     2761 acacttcact tggtccgcag agttctattg caacatattc ggctgattgt tcttttttcg
     2821 gtttgtcctg agctatatct ggcctatgag gaacaaatct aagcgtcacg gctctgccgg
     2881 ccatagattg gccaagctga agaggcttgg ctccttctac ataagcggaa ggccagcctt
     2941 tgacccaaag cccatcaatt agagtctgtg taggtatttt cttcaaaact tccaaggttt
     3001 catctgcaat aggcgttatt tctgtcattt cgttcctctt attcaaattg aattactatg
     3061 cacctatcta agcatataat tgaggtaact atctacttcc cagatttggc ctgaaggagg
     3121 attatcaaag tggaaaccct aaacactgac atacttgtga ttggcggagg ggtaaacgga
     3181 accagcatag caatggcatt ggttgcgaga agtgcgggga aaataacatt gattgaaaaa
     3241 ggcggtcttg caacaggtgc cactggacga tctggtgcaa tggtgcgaga acactatttg
     3301 aatgttgaac tcgtcaggat ggcttctgat gccaaaaatt tctttgaaga atggtccaag
     3361 gaaatgggat atgatctcaa atttcaaact acaggaagga tactgctatt tgatgaatct
     3421 gacgcaggcg ccgcgatagc aaatggcgag atgaatagag cagaaggggt aaatatagaa
     3481 accttgagtc ccgaaaaaac aatggggctt attccattgg caaacctaga agacgtaagt
     3541 atctccctgt acgaaccaga ggcaggataa gctgatcctg tagctaccac ttatgcgttt
     3601 gccatggaag ctcaaaaaaa ggcgttgaat tattgacccg gacaccagtg aaaaatctaa
     3661 tcgtcaaggg cgggcgcatt ttaggagtag aaactgctac tggaaaaata aatgctgatg
     3721 ctgttattaa tgtgactggc ccttgggcca attccttgct cggccaaaat gatgtcccac
     3781 ttccaatcac tccaattaga gtacaaatgg tccatctaag acgtcctccg aaacttgaaa
     3841 cattagagca aatcatcatt gactacactt gcggggctta ttacagaaca gatggcacac
     3901 ccaacacact tgtgggggga gaagctccag aagatatgtc ggagatagtg aatccaaaca
     3961 catttggact caatgcagac catgatttca taacaaggtt ttggaacagg gccacaaagc
     4021 gttttcctga ctttgttgat gccacctgca ggggtggata tggatctctt tatgatatga
     4081 ctccggatgg aaatccaatc ctggatgaat caccaaacat ttctggactt tacaatgtag
     4141 ccggatttag cggacatggt tttaagttgt ccccaataat tggtgagatg gtcgcagatt
     4201 tagtgacagg gcagtcaaaa agctcgcacg attacaaatt cttccgatca tccagattca
     4261 atgagaactc actaataacg cctactcggc catactctgc gagggtgcat cagtaaatgt
     4321 tgttatagac agccaatccc accatagcta taattttctg ccacggtggt tgtagttggg
     4381 gacactttat caggccgagg tggtggaatt ggtagacacg cagcgttgag ggcgctgtgg
     4441 ccgaataggc tgtaggagtt cgagtctcct cctcggcacc actgaaattt ttattaggcg
     4501 acgtagccaa gcggtctaag gcgggggttt gcaaaacctc tattcggcgg ttcgaatccg
     4561 cccgtcgcct ccaaaaaaat tcttcatcaa gtaaggcatc atatgcttca gcaccaacat
     4621 gaatccccta tttggataat ccgaacttcg taatgccact gaaagaatcc tctgaattag
     4681 ttctgcttgg aacaggaggc ggtcccaaaa tatgggcagc ccgatctcaa cctgcaagcg
     4741 ccatattagt caatggagat gtttacataa tcgatgccgg cgatggtatc accggacaat
     4801 tagccaaatc aggaatagat accaactcca tcaaagcggt attcataacc cacaatcatt
     4861 cagaccacgt agctgactat ggaaccttac ttcttcgggc ctggcaaagc ggacataaag
     4921 gtcctattga ttgtttcggc cctccgccat taaaagatat gaccaattca tatatggcct
     4981 atatgaattg ggacatcaat cttagaattc aacatgaagc acgacctgat ttcaaatcaa
     5041 tgatcaatgt tttggagata acacataact catccgtcta tcaagactcg aacgtgaagg
     5101 tagattgcat aaaagtacct cacggagagg ccgatccatc ttactcatat cgttttcaaa
     5161 tcgatggaaa ggtaatagtt ttttctgggg atacttctaa aagcgaagct ctagtaagtt
     5221 ttgcttcaga agcagacata cttgttcatg aagttcttaa tcttgagggt gtggaggcaa
     5281 ttatagagaa aacctatccc ggaaacgaag ccttcaggag tcacataatt gaaggccata
     5341 caacaatgtc cgaggtagga gaaatagcca gccaggcgaa ggtgaaaatt ttagttctga
     5401 accattttgt gccgaccggt tctccgtatt tggacaagga agaaatatgg caagatgggg
     5461 tacgcaaaac ttttaacgga caaataatcg tgggcactga tttattaagg atacctttgt
     5521 agttcaaaga tattgtagag aattaaccag ttagaaaaag aggcttcacg tgttccgcca
     5581 ataggtccat tgtccggaac acaatacttc gatccatttc tccagtgtta atcttcatca
     5641 tgagattttt cacacctatt ttatccaatt ctgaaatctg ttctgctaca tctcttggtg
     5701 tgcctgtgat aaaaatatta tcgaagtctt tttttgatat ttctctaggt tttgatagac
     5761 ctggcgatcc ggatttgggg ttatatttat ccctagcgac cttgaaaaaa gtctgttctc
     5821 ttgtatagcc gtcaaaggct atttcttccg cttcagaacg actttcagca accacaatgt
     5881 ttttgttaac ccacacttgg tccaaaatat cctccattcc cgactcggtg atttccgttg
     5941 attccaacga ttttgtaaag acttgaagtc tatcttttat agaatgatta tcctgagctg
     6001 ctatcaaaat tggacgtctc ttgaacccca tttcaattgt ggattccctg ctgatacaag
     6061 cccgcaacaa aggagggtga ggtttctgta atggcatcgg tctcagttcc ggaaatgcga
     6121 gatcccaata tttaccttta aactgcacag gcttcccact ccatgcctcc aaaataatct
     6181 cttcagcttc tgaaatgcta tctgcagcat cagacattgg aaccccaaac ccaatatatt
     6241 catattcatt aaaagccgat cctttacctg tacctaatat cattcttccc ttgctcagat
     6301 ggtccagcaa tgccacttgt gctgccagcc tgaccggatg atgcaaagcc atttcaacta
     6361 cagcaaaacc tatagttaca ttctttgttc tctgtgctat tgcacccgcg aacaccactg
     6421 ggtcagcata ggcagtagct ccatcaaaat ggtgttcaga taaccaaatt gaatcaaacc
     6481 ctagagcttc ccatctatca atttcagtta aagtgtcgtc aataacatgt ccatcatttt
     6541 ttgaattttg agatataggg aacacccaag aaccgaatcg cataaattac acaccttaaa
     6601 taattaatta cacattatga aattacagtc tacagaatat ttcatcacct aaaagtgaat
     6661 aaggcaccta gtaactacta tcggctgcac tttttttgag aatatatttt tttaacataa
     6721 cttcaatttc cttgcttgtg tacaagtcat caggacctat cgcaataaaa tccagtattc
     6781 ctccgtaaga gtaacttgac caccacctac ctaaattagc catgcaaata tcctctaaat
     6841 attacttgta ggtcgaataa atatttactt gacggtccaa cataacaaag gttcatcacg
     6901 agcgggcttg agccaaacca cagacagttg aggtgcccac tctccctgat ctgtcatata
     6961 acgacatagt gccaacagaa actccttgat ccgctccatg ataagaagtc tccgtaccaa
     7021 tccaactccc tatgggagtt cgctgaaggt acaaagttat atccgcattt atgtaactca
     7081 gccctttagt acctgaattt gtaaatggat tagcaaaatc agccacctga gctatcctta
     7141 ccaactggct tggaagcatc cctgatatca gactatatgt ttcccggatc caggcccttc
     7201 ttacccctag tttcctgttc tgtgatctat attcgtcata tggctcccct gtgtcatctt
     7261 gcacgtttac agtctgccat attggtaaag aggccgtacg gtcaggacct ttctgagcta
     7321 cgtatggagg tggcatatct tcaggtggag agcgaatttc ccaggcagta ggagaccata
     7381 tttccccatc tggattctca cttttcttca gcataacgac acttcccctg gctatatcta
     7441 taatacctct ccccttgatg tctgatgcaa tatttacatc gacgaccttt attctcctac
     7501 cggatctaac gatttgattc gtaacaatca aaggtgacat aggaggcggt cggaaaagat
     7561 caactgtaat tcttgaggcc tggaaagtat caacctcatc ttctgacaaa caattcgtct
     7621 caacctcaaa agccaccaac ccagacaaga ctcgcccgtg aagtgttttg ggatcccatg
     7681 gccctcgtgc ataaagtgtt gggtgataaa catttttatt gtcactatct cggttaaaaa
     7741 aggcttcatt catatggcta tgacaccgtc catatttcac agattttatc taaagcttcc
     7801 tgtggcagtg gtccttgatt agcataatct attgcttgct ttaattgatc aaaattcgac
     7861 agtccgatta aagccgtatc gagattgtca tgactaattg cgaatctaat agaggcttcc
     7921 acaagattat ctacgaatcc ctctttaagc agaaattcaa aagcggaagt tctagtaaca
     7981 tcttcttgaa acgtggatga agttgcgatg ggggccacgt tttgctgtgc gatcggatgt
     8041 ctcttcaaac tacctgatag agctcctcca gccaaaattc taataccgac agttccgata
     8101 ccctctgttg atgctaactt cataagctcc tcatagtttt gatatggaaa tgttttctca
     8161 agatggtacc cagaagttgg attgagcatg ttgtagcaag tatgtattcc actgggggaa
     8221 tacttaacta atgcctcttt tatagaatct gtttcgccaa gtccattaaa tccccagtac
     8281 cgcaatttcc cagaggcaac agccttttca aaaacctcca ctattaaatt taagtcatcc
     8341 acagaaagag tccctgaaga aatgtttcgt tcagcgccta tagtattatg ggtgtaaaca
     8401 atatctactg tattaatccc aagccttagc aaactattgt ctatttgatc tgaaatagtt
     8461 ttcgaaatat tttcaaattc atcactcgca attctcactt tcgtcccgat gagcaaatca
     8521 gtcatgtcta gttctcttag aatggctcca gtatgaattt cagacaatcc gtcaccatac
     8581 atacgagcag tatcaaaata attaatgcca gaatcgatag cgtattcgac cgcgtccatc
     8641 atctctttgt gatcacctaa aaccatcagt ccaccaacgg ctccgcatcc aaaacctaat
     8701 tgtgaaactt gtaattctgt atcaccaaat ctattaattt tcattcttta tttccctgtc
     8761 ttgcagaccc gttctgttag gtgattccac atatatttat gtttttaaat attggaaatt
     8821 tcagcctgat tcgtcatttc atagttctct caatgcatga tcattccacc atcaacgttg
     8881 aaagcttgcc cagtaatatt cctagaacca ggtccagcca agaaaactgc cagggcggcc
     8941 acatcttctg catcattcgg tctgccaaga ggaatttcct cagtcatcat cgctttaacc
     9001 tcttctgaac tggccccgag ttctgtagac cttaaggaaa gaatgttggc taacatatca
     9061 gtttgaacga tgccagggca tatcgcattg acattcacgt catgttcagc catgacacta
     9121 gctgcactat gagtcattgc aattactgca cctttactgg cagcgtatgc agcgtttgat
     9181 gctcttctat aacctttccc agcaatggag gcagtattta taattctgcc acctcttgct
     9241 tgttctacca tttggttcgc gaccttttgc atgcagaaaa aagcaccttt tgcattaaca
     9301 cgatttattt gatcccaatg atcttcctct atatcaaaaa aacttatgtg gcgagtcaaa
     9361 cctgcgttgt tgaacaaaat atcgactgag ccaagtttcg aaactgcctc atgaaccata
     9421 ttttcaatct ggccaatgtc accgatgtcc aacgaaattg aaaaggctct gacccctaga
     9481 gccgaaattt cctcagctgt atctttggca gtctgaggat ctatatcagc tataactatg
     9541 tccgctccac cctctgcgaa tttcgttgcc gccgcttttc ctattccccg gccaccacct
     9601 gttaccaaag ctaccttacc ttcaaagtcc ataaaatccc tcttaattta aaatcttgta
     9661 tatccgccat caattacaaa ttcttcaccg gtttgatacg aggaagcatc actggccaaa
     9721 tagaccgcaa tcccagcaaa atcttccgga gtgccccacc ttctagcagg cactcttttc
     9781 ataaccctat cttcaaatct ctcgtcccct acccgagctg aggtcatagc agtttcgatc
     9841 caaccaggaa taatagaatt ggcagtaata gcgtatcgag ccatttcaac tgacaaccct
     9901 ctaaccatag ctagcattgc tcctttggta gatgcataag cttcatttct agcagcccca
     9961 tgaatagcgg aagtactaga agtgccaaca agcctgccac cctcccctct ttcaatcata
    10021 tgtttagacg ccgccctgaa tgtaaaaaag gtaccatcaa gattaattcc cataaccttt
    10081 ctccaaagat cagtaggaaa ttcatgaaat ggagctcccc caccagcccc agcattagcg
    10141 aaacaagaat caactttccc gaaaacttta agagtttcct gaaaagccgt ttcaacttga
    10201 tcttcggaag acacgtcaca tctgattgca tgggctttga ttccaaggga actcagttct
    10261 ttttgagatt tttcattctt ctcaatattg gttccccata tacaaacatt ggctccagat
    10321 tcagccaaac cctttgccat ccccagacca atcccactat taccaccagt cactaaagcc
    10381 accttgcctg ataaattaaa tgcatctaaa ggcatgactc ctcctttgtt attaaccgtt
    10441 tcgatataac tgcattttaa aatagatcaa ccttagaatt ccaaataggc acaaaggtag
    10501 gctgaaatat tttacaaatg gtcaaaaaat atacgccgca gatatctata tttgaacata
    10561 cactgcttca agtcctctat acctctgctc gctatccatc ccaaacccaa ccaaccaacg
    10621 atcacttttg agttgaaatc cgcaaaaacg aggaactatt tccttgggtt gatttctttt
    10681 ccgatctatc aacacagcca gatccacttt ttggatccct cttgattgta tatctttcat
    10741 taatttcatc atggtagtcc cggtatctag taaatcatcc accacgactg ttggtgcttg
    10801 taaatcaacc cgattatcaa aagttgtggg gaattctatt gtaggttcgg ttaaaacttc
    10861 gtcactagaa taagctgatg caaatattgg agacactagc catttaccag gtcgtttctt
    10921 ctccaattcc gacaaaagat aagctgagaa ttgcattcct cccgtcatta ctggcactac
    10981 attgatggta ctgagattat cgtaatactc aaccattgat tgagccaacg aggcgaccgc
    11041 tgattgtact tcgtgcttgg aatataatat ttctagacca tctaattcaa aatctgtatt
    11101 ttgatccaat ttattctcct aaataagtca tttgatgagc tttttgctgc atttgtacaa
    11161 ctcccagctt ctataggcgt tgaaatgatt cgcctagagc cgaggggggc ctagcagtta
    11221 tgaatcgacc aaacatgcta ttttttaaaa gcaggtattg aattttttta tggttgataa
    11281 ataaaagtgt catgatcatc aatggaaatg gaattatcgg aaggacttga gaaagttgag
    11341 gaaggaccgg ttgtagttta agcgccacca actgcaatac accaaacaaa taacatccca
    11401 atgccgccct tataggatgc caccctccaa aaataactat tgccaatgct atccagccaa
    11461 aattaatgat atgcccatct gaccacccga gttttacatc tagagaaaaa gcggcaccac
    11521 ccaaccccac tagaaatcca cccaacgcag tgtagaaata tctcaattta ttgacgtcta
    11581 tacctcttgc ataagctgcc tcagagcgct ccccggcagc ctgtaaattc aatccctgcc
    11641 ttgtatggaa aataaaccaa aaggagagtc caataagtat gtaactgaaa tatacgagta
    11701 agttttgttg aaataatacc ggccccactc caggtatgtc tttcaatacc gggatgtgta
    11761 aatcttgcac cgatgggcct ggttttctca caaaatcttg tcccaaaaaa gccgctagtt
    11821 tcgtagaaag taaggttagt actatgccaa ctgctatctg atttaatcgc agtcgtatag
    11881 aagaaaatgc aacaattagg gctagattgc tcgaaaccaa tcctcctgct attaaaccaa
    11941 ggacaacatt tccagtagta aaagaaaccg caaacccggt catagctgaa agcagcatac
    12001 ttccgtctag ggataaattt actacaccag atttttcggt gatagtctcc cctaatacag
    12061 cgaaaaccaa aggggttgcg tagctaataa caatagcgag ggtctctgga ttcatctatt
    12121 attctgcctt agcatgtagt tctcttcttt gccgaaggag tctcattgca cttattaata
    12181 ttactgagag caccaatgtt ccctggaata ccccacccaa ggaagagtga agacctaacc
    12241 ttagctgcca ttgaatgcta ccaatggcga tcatagagaa gaaaaatgct attggtatta
    12301 ccaaggaagg tctatatccg gccaatagaa ccacgagaat ccccaaaaat ccgtatccac
    12361 cagatataga agggattaat ctatgatgta ctgaggctac ctgtgtcact cctgccaatc
    12421 cagcaacagc accacccaat ataaacgcca tcaatatata tttggatgtc gatataccaa
    12481 acctgtaact tgaatctgga ttcaagccta ttgcccttaa cctaagccca aattgggtcc
    12541 tattgaaaac aaatagaact gtaagcacaa ccaacaaggc tatggacact gcaagtggcg
    12601 acagcctaag tccttcgata gtaggaaacc aagcactgcc ttgaaacggt tctgttccac
    12661 tggtggaggc tattcccttt cgactccaag gaccaattac aagataaact acaaggcctg
    12721 ttgcaacaaa atctaaccca agaccaccga aaatctcatt aactcgtcct ttaactttaa
    12781 gtaatccgac taaaagcccc cagaaacctc ctcctaaaaa tccggcaact atcattgcag
    12841 gtaacaaaaa cccagggggc atatggattt ctctagcgat aaacgaagct gccactgccc
    12901 cagctattat ttgcccttct accccgatat tccaaaggcc agctctgaaa gttataacta
    12961 acgaggccga agctatgatt agtggaacac taaccataag tgtgtcacct aacttggtca
    13021 tggaccccaa agaccctgtg atcaaaagtg tataagtttc taaaataggg atcttgaaca
    13081 aggtcaaaag aatggaaaca aaaaccatag ataccaatac tgcagtggaa tagtaataaa
    13141 tgggaccagt gacccctttc atgattggac gccgcctatc aaggaaccaa tttcatatgg
    13201 ggtcgtttct ttagatgata ggttaccaat gattttccct gaaaaacaca ccagtattcg
    13261 atctgaaaaa tttattaatt catttatgtc tgaagatgaa aatattatgg tggttccact
    13321 ttttgaacgg tctaaaagtt cctgccaaat ccattccgca gattctatat ccagtccccg
    13381 agtcgggtgt tccatcgata aaacggacaa gttatctggc atgagtgcta gcaaaagtct
    13441 ttgttggttt ccccctgaca aatcttcggg ttttgaggta acagttcctc gaatagagta
    13501 ttcctgcaat ttacattcag ctatttgtcg agattccttc caatctattg ttttacgact
    13561 ttggttcccg gttaaggcaa aatgttcagt tatcgtcaat ccctgaatta aaccttctcc
    13621 tgctctatct gcgggaagat aatggaaccc gttttccatg taccatttat aagaatggtt
    13681 cgttatattt tcccctttga aatacgtgct acccctgtaa gtttttgtta atcctgtaat
    13741 gtttctcaac aaaagacgct ggcctgcacc ttccaagccc gctaatccaa tgatttcgcc
    13801 ttcagaaaat ttacaatgga cctcttcgat ggtcgaggtt tcctcctttg cacaaatacc
    13861 atccaaaata agtaaatctt gacctagaac agtttgattt tgcgggagag attgtgtgta
    13921 acccccaaac ataagattca ccaaatctga ggtaggacaa ggtacagtca gagtatctat
    13981 caacactcct tttctcagaa cagttactcg atcacaaatt gattgaattt catccaactt
    14041 gtgtgaaaca aaaataacag acattccgct tgcagcaagt cgttcgatag tcttgaataa
    14101 attttcccgt tgggcttctg aaatagccgt ggtgggttcg tctagaatca atactcgggc
    14161 gcccatccag agtaatctag ccatttccaa ttgctgtctt tctcctatgg ttagacttga
    14221 agcaacagtg tccgggtcta gatcaaatcc gtattcttga gatattttgt gtatatcatc
    14281 acgtacaagt ttcctatcaa aatcacggga tttgggtaaa cccgcgagaa aattatccac
    14341 aattgaaaac ggaagaaaca ctaagggttc ttgatgtaat attccaatcc ccgcctctat
    14401 cgcatctgta ggagacgaca gatccaggta ttctccatca agctcaactg tccctgcatc
    14461 gcttgtcaac gatccggaga ttatgtttac taaagtagat ttccctgctc cattttcacc
    14521 aagcaatcca tgaatctcac cttggcctat atcgatagat atatcgttat tagctaagac
    14581 ggttccaaaa cttttgctta tattgagtaa cttcaaagac atgaagtcta cctagtttct
    14641 ttctattcag ttgttgatgc cccttcaatc ccttcaagca acatagggag ataccaaatc
    14701 tccttatcgg tggcttcttt accatcagcc aaaaaagctg atccatcttg ataatttata
    14761 gggcccctcc aaagatttag ctcgtcttta gccaattttt gtatgaattc atccagggat
    14821 tttgaattat ctgctgttaa gccctcgcct ttcataaagc ctatagcgct ggagtcatga
    14881 tcattgatat tatcccagtc tgggcccacc caatcgaacg aagtcttgaa atttccgtcg
    14941 attactgatt tagctatctt gtggtaagaa ggtccccaat tgaagtaagg gactcccaaa
    15001 cataccgcag gtgcctccaa acatgcacct tcatagtcgt aaggtactgc ccatactgtt
    15061 ttccccgaag ctgctgcttg accagcaaca acaatggctt ctgtagtatc gattcctgag
    15121 attactacgt ctgatccaga tgcaatgaaa tcgtttacca caacagttgg atcctgggtt
    15181 acacctggaa tattgaacca aaaacctatc caggtcactt taaatttcaa atcagaagcg
    15241 cttttccctt ttaccgtttc ccagcaatgt tttgctccta agtaggttga gctaaccagt
    15301 cgtctcgttt ccgcattaat caaaggacca agatatgaaa tattaccagt atccgactga
    15361 agggcagcag cacatcctgc aatcattttc ccgtattcca tcttccccat cacgttaccc
    15421 aaacttttca aatcactacg ataatcctct ccatctgacc aagctgtatc acctgaagcc
    15481 catatcattg gagtatccgg atgggcagct gctgcctcca aaatcccatc tttcatatcg
    15541 tcagatgttg ccaaaattag gttagcaccc tgcgaaatca tgtcctccac aatttgaggg
    15601 actgtaacat tgggggaatc agcggggtta actttgtcaa ttgtgataac ttctccacct
    15661 gtcttactaa cagcatacga ggcaccttcg aagtgtgctt gcgaccaccc gtgatcattc
    15721 tgtggcccaa ccagtatcaa acccaatttg aaggagtcgc tatcaacacc gccactacta
    15781 gaacaggccc ctaaagacaa aagtaagatc aatattagag gcaacaattt gaaacgcata
    15841 agaggttctc ctttgaattt aactcttttc gggcggtcat ttttgacaaa atcggcaaaa
    15901 catctctttg tcattaatta tgcacacaca agtctatgct tcaattggag atagtggata
    15961 tcgcaactgt taaacttttg taaactgtga gcacattttc tagaggtatg gcatcacttt
    16021 ttcagcgaac aatttcatgg aatgctgaac cacttcagtc gtatttctac cccttctgtt
    16081 aaattccaat atacattgat ccattccggg aattctatct tctagttccc ttagactctc
    16141 tatgcaacct tccgggctat cgtaaatgca gtgttctagt gccgttgcaa aattccaaga
    16201 cccccaacca ccagcacctg atgagaagtc atgccgacca cctcctattg ctgaagaatg
    16261 cacaggaggg ccatcgccaa tcacatcact gtcaagccaa tttttccgca tcaagtcatg
    16321 ggctttttcc ttactctcag ccacaaaaaa agaaactaca acatgaaaag gttgcgggtt
    16381 accatttgct gcaccttcgt ttacattggc tttgaaagtt tttatatgcg ctcccaagtt
    16441 agctatcgat tggcttaaag ttattccaac cacacccaac ccgttttgcg cagcaaattt
    16501 gaaagaccca tcactatttg ccgccatcaa aagtggcggg taagggcctt gaactggctt
    16561 tggaagaact gggacatcat tgaaatccca atagtttccc ttgaaattta gtctgctctc
    16621 agcccaagca tttttgagga catcagaata ttcagaaacc atggctctac tttcgtcacc
    16681 gtctatttct gaagatactc ccattacttg actagacggg ccaccccatc taccacctat
    16741 gcacaagtcc aatctacctt tcgtcaaaat atcgcaagtt gccacctcag tagctaaatt
    16801 taatgggtgg tgcattggaa cctgggtcgc tagagtacca aaccgaatct ttgttgtatg
    16861 ctgagccgcc gccgccacca ccaataaagg ttctgacatt ggcctcgtcg gataaaaagg
    16921 tgcttctgcc aaccaacctt caacaaaacc caggttttca gccaaaatca actcttcaat
    16981 ggtttcccag tgtcgacggc ccacatccga tccctcatag ccgggaactt gccaaaatac
    17041 tccaaatttc aaattgtctc actcctggaa tttattccac tggtatggct atggatactc
    17101 tggtcctatc cccaaccgcc ttcgtcgtgt gaccttgtcc cgttaaatct tcagcaagca
    17161 tcatatctcc agggcccaag gtatacacag atccatctcc taggcctatt tctacttgac
    17221 cttccaaggt aactataaat tgcttctttg gggccacatg ccagtcaatg aaatttccag
    17281 gttcatgcca ccgaaaaaca atacctgttg ctggcaaatc agcaggcgga gaatactcct
    17341 ccatgtggga ctgtccgtca tctccggtga aaattctata aaacattctt accccctaat
    17401 actagaagtt tctttgttta caccaaatat cttttcgtaa ttgatcatca aggatacaga
    17461 gcatccaacg aataacaaca taccgctcct ggtgaaattt aattttatgg gcgtatatct
    17521 agcttccatt cacctgatgt agatgaaggg gatattttgc acttaatctt tttttccaac
    17581 gattttggga gggcgtaggt accaaccatg tgcgaagagg ttaaaaaggt ggttccaact
    17641 ccatattgag cctcatttat cgcttcatca acacgagcta tttgttctgg aattgataaa
    17701 ttcgtcatat ctacagtttc ttgaggtttc cccattttag ggaaaccttt tgatccactg
    17761 gacacccagg taggctccta aagctagccc tacggcatat acccatccat tgagtgctag
    17821 agagggaaca gccgaaaaga aagcccccaa tgtgcaacct aacccaagcc cagcaccata
    17881 tcccataatc acccccccca ctatcgattg cacatatcta accggagttt gtggaattct
    17941 caatttgaac tcacgggcaa atacagctga tgccaacgct ccggcgacaa taccaaaatt
    18001 caggaaaaat ccgccggtga gccaagatcc ttctgccact gtcatagcac atgcccctaa
    18061 accctctata cccttcaaat gtagttcttg cagtcccagg ttagtactca aagatgtcgt
    18121 ccaccgggat atttctccga caacccctaa aggcctaaac ctgacaaaca ataatatatt
    18181 gattattcct agggctatcc cacctataat cggactccat tccttcctaa aaatgttcct
    18241 caaataccct gttatttctg aagttgtatt agcaggattg ttttcttgag attttctttt
    18301 tatttctggc ataatgaatc ccgcggtagc ccttctttcc ctccaaacag caagataaag
    18361 taaggctgcc agaaggaaag tggtgaccac cagagcgcct gtataggaga tttcagccgg
    18421 catccaaatc tgtgcttcag aagaaatcaa attgtcccac caccaattcc aggaattatt
    18481 aagcaggaag agacccacca taactccccc tatggcaagc caagacccca cataaccttc
    18541 tcccatccta tatagggatc ctgacacaca tcctcctgcc aaaaccatac cgatcccaaa
    18601 taaggtgcct gcaattaaag tagacaatcc cacaggcaac acattggcat ctgacggggg
    18661 gactccaaat cctgggtttg gcgacaccgt gctcatcacc attacaaacc ctacggtgca
    18721 aacagcaaga ccaaccaaaa ccccctttaa tattcgagtt tgtcccagaa ggaaaaaatc
    18781 tctaaaggcc gatgtgaaac aaaatcgact tctttgcaca gttataccaa aggcaatacc
    18841 agctaaccaa aaaacagcct ccccatgagt accggcatag attgtgtacc caagaattaa
    18901 taacaaaacc cccgacacga caagggccca aaaagtattt ttatctatct tcccttttct
    18961 tgacgctgta gctatctgca taaattccta ctgctatttc ttgctgatgc tatgtctccc
    19021 tgttggcatt taaattaaac gaaggcgcat ctcaaccagc gtatccaaca agcacaattt
    19081 atatagacta actaagtacc cccatatact acataaattt tttcttatga atagtgcaac
    19141 ccagcagatc attaagttta atttgacata aaaggtacta ttcggtaact ttatagcctc
    19201 atgataacta atagaaaata cggcttactc aagcagacta tgtgcctcgt gagtgcatac
    19261 cgtcttgcag gtaagtgttc ttgtatgaat taacaagggg tatttttcta gtcgcttaat
    19321 ggcgaataaa cataaccccg tccgcattgg ctggacgggg tttttttttg aataaacaca
    19381 aaggagtttt cgaaaaatgc caaatgactc aaatccaatt ttgctggacg taagaggtga
    19441 aatatgccct tatccaatgc tcagaacgaa taaggaacta gataattcga aacaaccgct
    19501 gacagctttg gatgtactaa ctgatcaccc accagcactg tccactatac ctcctgaagc
    19561 catgaaacga gggtatgggt gcgaaattga agagatggaa gccggtgaat ggaaaattcg
    19621 tttatttaaa ccagagtaga gatatctaca taaaaaacgt tacaggagca cagatatgct
    19681 gatacaccga ttgaccaaaa tgggttttgg aatgctagta ggagcgctaa ttttaggcgt
    19741 cctaagtgcc tgctcaagca cttccggtgt gtctgaagac acatcattgg atagtagagg
    19801 ttactcatcc acagagcgac tggtgtcaac aaaatgggtc gcagataatc tcgacaacaa
    19861 aaatgtaaag gttattgata tcagaaaagc tgaagactat gacgctggac acattcctgg
    19921 tgccttaaac tactcttcca aagaactcca ggttgaatct ggaggggtga aaggtttaat
    19981 cccaccatca acagatattt cgaataaact tagttcttta ggtataaaaa gtgacgacac
    20041 cataatcatc tatgacggca taaaaaatct ctggtcaaca aggtttttgt ggacactgga
    20101 tgtatacggt catgatgacg caaggatgat ggacggttcc tttggtttgt gggaaaaaga
    20161 aaacaggaaa attaccgccg atgctactcc agcaaagacc tctacctata aattcacagg
    20221 tacggccaat agtgaactgg tcacaagtat agagacagta ctgggttcct tagatgattc
    20281 ctcctcggta gtcctagaca ccagaggggc agaggaattt gcaggacgtg atgtaagggc
    20341 cagtagaggg ggccatattc caggttccat acatgttgaa tgggtgaaca acgttgatga
    20401 agatggtaga tttctacctg cctcagattt aaaagaattg tatgcatctg cgtacataac
    20461 ggacgagtta ggcgatattt ataccttatg ccaaacggca gtcagggcaa cccacagctg
    20521 gtatgtactg gctgatctac ttggctttga taatgtatcc gtctatgatg ggtcttggac
    20581 tgaatggggt aacagagaag atacacctat cgactcctag atcagactag gattctacta
    20641 ggacaacagt aaagcactgc tcatgttgct catcaaccca aatgaataac atgggcagtt
    20701 ttcaatttcg cacgataccc aataataaga attaatagga gcatcagtat gggaacttta
    20761 gatggcaggg tagctttagt aacgggatca agccgaggaa ttggagaata catggctctc
    20821 cattttgcaa aagctggagc caaagtggcg attgctgctc ggactactga agtaaccgat
    20881 aaaagactcc cgggcaccat ttattcggtg gcagaatcaa tcaataaaga tggcggtgag
    20941 gcaattggag tccaaatgaa tatgagagac cctgaaagta tcacagccgg ggtcgatgaa
    21001 acggtggaaa aactcggggg actcgatata gtagtcaaca atgccgcgat attggttcct
    21061 ggatcgctcg aaaccgtcca agacaggcac atcgatttaa tatggcaaat agatttacgc
    21121 ggaccattaa tcctgatgaa acgtgccctg ccccacctga aatcggcagg tgcgggagat
    21181 ttcataaatg tatcttcagt cgctggcgtt ttcccaggac caggacctta tgaggacacc
    21241 ccttctagaa gagacgggag tttttatgga atgataaaag ctgggttaga gcgattttca
    21301 caggggcttg ccaaagagtt gcaaggagac aaaattaaag tgaatgtact ttcccctcaa
    21361 ggtcgtatta aaaccccagg aaatatatgg gccgaaaacg atccggaaaa cccatccctt
    21421 gaatttgaag ctgctgatga aatgggcaaa gcgggtgtat ggatatgcga acaacctaac
    21481 acctatactg gccatatcct ttttgatcaa gatgtttgta aagagcaaaa tttgtagtaa
    21541 ggaaacagat agccattcca atataactat gcctctgcaa catctacggt tacttcatac
    21601 ctcagacaca catcttgggg atcatctagg tcacccgtcg tcgaacaacg ccttacagaa
    21661 ggttgtatct gaaacaattc tcttaggagc agattttcta ttgttggccg gggacgtatt
    21721 tgataacgaa agaatatctg acgaaactgt aaaatttttc ttggatgaaa tatataaatt
    21781 agaaacacct gtaattttat taccgggtaa tcatgatttg atgcacgaca catccatata
    21841 taaacggggg attttcaaag attatcccag caaccttttt atatttaagg aggaggacgg
    21901 tgagattatt gaattctatg atcaaggtat caaattctgg ggtaaagcca tgtctgagca
    21961 cagtccttct ttccaaccgt tgtcaccgct ccctcaccct gaaaaaaaca cgtggtttat
    22021 aggcatggcc cacggacatt tcgaggcgat agaaaccaat tccgggaggt catcattaat
    22081 atccccagga gagatcaaca aatcaccttt taattatatt gctctaggcc actgggacct
    22141 ctacaccgat gtatcccaag gacaaaacct tgcggtttac tcgggatgtc ctatgaccat
    22201 aggggctaaa agtggaccag ggtttgtgag tcttgtggat ttggatcctg aaaaaggaac
    22261 actctacgag aaatgggcca tcaaatctta attcataccg acagttgctc caccatctat
    22321 tacaaccatg gcccctgtta tatacgatgc ctccgccgag gccagaaata aggctaaatt
    22381 tgctacttct tccggtttgc ctacacgatc cattagcgac atggatgacg cttcctgaat
    22441 aaatccttct gggtctgccg ggtctatctt tttttgtcta gaaatgcccc tttgatacca
    22501 accctcagta tgtatatctc cagggcatat gccattaacc ctaataccat attcccccca
    22561 atccatagcc aaacacctcg tcagctgggc tatagctccc ttggtagagt tatatggcag
    22621 tgattttgcc tgggcaataa aactactaat cgaagcaata ttgacgattg tgggatgatc
    22681 acttttcttc aaatatttga gggtagattt acatacattg gcagttccga tgacattcac
    22741 taaaatggcc ttcatccact cctggtaatc caaaacatct gccccgccaa aagtaaacga
    22801 agcggcatta ttgactacag aatcaacacc acccataact tcaacagcat attccaccat
    22861 tttttttacc tctgccatgt ccgacacatc agtcttacaa aaatgaactc ccccaccgga
    22921 actagatgcc aaacttggta taatcccatt cgagtcttct ttaacatctg ccactaaaac
    22981 gatggcacct tccttaataa aacgactaac tattgccaat ccaataccag acattccgcc
    23041 cgtaacaatt actttttttc cttctaatct catatcagtc atggaatctc gttttaccac
    23101 taccctttat ggttttcgat ccgttctaga tctgttttgg taaaatatgg atcgagtaaa
    23161 gtgaccactt cctctaatgt gtcttcagga ctatctatgg aagtatccac cttaactact
    23221 ggccctatgt cacttttcga tatgaaatat tggtaatcag ccattacatc ctcgatatct
    23281 tcaacctgca ggggactatt agtatgttct gggtggtctt tcagagcgag cattcgtgat
    23341 tcgatgatgt cgggggaggc aaccatgtgc accgttatta taggatcagt ggtaatttgc
    23401 tttatacgat tttcaatacc gacgaaaaca ttgttcctgt cgaattgttc tccgtctaac
    23461 ccataaccat aatatcttct tgcataaacg gcttcttcaa tgtggaaccc caccgacaaa
    23521 tcatcttctt gaacatagtg gtgtatgtgg taatacattc cataacgatg gtacatctct
    23581 tttaattttg gagacaaatt caatatttgt tcctgttctt ccaatgtggt gtcatcagga
    23641 tgtccagagg tatgaggcag tttcgaatgg tcgtgtacca atcccataaa aaagggttcg
    23701 cccatgtttt ttaacttcca gtcactcagc attctggaaa tagtagtgga tccgacatat
    23761 tcaataccag ctaatattac tttcataact caattcctta atttgtactt ttcaacctag
    23821 aatggataat cacacaagtg tttgattttg tatagatcga actctttcta cttgctcaac
    23881 cctatctcaa aattgcgccc aatttacgac tatgctcact attggctctg atatactgaa
    23941 tatttgaaaa ttcatcgaca gg
//
LOCUS       DASCNS010000137        13897 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001133434, whole genome shotgun sequence.
ACCESSION   DASCNS010000137 DASCNS010000000
VERSION     DASCNS010000137.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001133434"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="QF838_04935"
     CDS             complement(<1..1415)
                     /locus_tag="QF838_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60062.1"
                     /translation="MKVLDAVARILKAEGIEWAGCFPSNNLIEAVAEVGINPIMFRQE
                     RGAAMAVDGFSRMRNREEFGVLITQGGPGSENTMGGLAQAYADNVPILYLPGGPAVSA
                     RSVKPNFSPARTYESVSVSAEVIFQPGQVASIMRRAFHALRNGRSGPVIVEIPADVGE
                     MEVDDSVVSSYSPPKRHKFAPDSAEIKEAVRLLLAAKKPVIWSGMGVLMSGASPELTE
                     FAEIAQIPVYCTMPGKSGFDQRHPLALGSGSSATSLQARTWLQEADVLLGVGTSLTRT
                     GYGQPIPDGKVMIHNTETVADLNKDFNIDVGLVGDTKLTLSMMVDEVKIQLAGAERKG
                     SSELSDQIAEINDKWMAEWSGVLNSNETPITPYRVIGEMINVLDKENSIVTHDAGAPR
                     DIIMPFYPGTVPHSYVGWGKTTHLGYGIPLMTGVKMACPDKFCMNIMGDGAFGMSGLD
                     LETSVRAGAPITTIVLNNGGMA"
     gene            1639..2397
                     /locus_tag="QF838_04940"
     CDS             1639..2397
                     /locus_tag="QF838_04940"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HJO60063.1"
                     /translation="MQIGQKIHHVLEINSTMEACKRLANLGEPNGTIVSANYQESGRG
                     RFKRKWVSPSGDNIQMSVLLRPNQQELKYLNIFASMAVLATCEQTLGVDGSIKWPNDV
                     QINGKKIAGILVETVLEGEKVVSCVIGIGLNVNLDVKPHAEIEETATSLKCEKGRYIS
                     RSVILKELIKQLDFYYSQISDGRSLTQRWCDKLSTIGKKVTLSFGKNPNNHILVGLAE
                     SISDDGGLIIRKEDGSLFTANAGEVTLARKEEIL"
     gene            2431..3237
                     /locus_tag="QF838_04945"
     CDS             2431..3237
                     /locus_tag="QF838_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60064.1"
                     /translation="MLEKLNLEDKTIVITGGGTGLGKEMCLAMARAGANLVIAARRLE
                     PIEEVSKLVRGLGRRSLAIPTDATNTTDIKTLFNTVLAEFGKVDVLINNAGIVREDSA
                     RPIWEITDESWKIGIDVNLSTAFYCSRGISKHMADNGGGKIVHVASGFGFRGGRDNYM
                     YAAGKGGIVNLTRAMATSLGKYGITTNCIVPGFIPTEVTDPNSDRSQQRGRFIPIGRV
                     GQPSEMGPLAVYLASAASDYMNGEFFAIDGGGLAGGIAPTLHAPEIPLEI"
     gene            3281..4039
                     /locus_tag="QF838_04950"
     CDS             3281..4039
                     /locus_tag="QF838_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60065.1"
                     /translation="MRTPEGWDLTGKKAVISADRRGWTKFFSSALAEAGADVAIIGSE
                     NSDMEEASNKASDYGHTVITQTTNLMSKASIDKSIQTINDAFGTIDILVNNAKANFGK
                     KFEKVTESEFDTLIDFNLKSMFLTCQSVGQTMLNKKSGRIINMTSDLAVRGLWNSVVP
                     CASEGAIHQLTSSLALEWGRDGIRVNGIGAGWLNTEQESDQSEPGLLERYLPSKRKGH
                     PNDLTQMLVYLSSDSCDFVNGQTIFIDGGALAHA"
     gene            complement(4125..4388)
                     /locus_tag="QF838_04955"
     CDS             complement(4125..4388)
                     /locus_tag="QF838_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014389.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HJO60066.1"
                     /translation="MGLYLANFLNILASVLYFSILARVLVSWFNVSPGSPFFPVIRLI
                     YAITEPIMGPIRKVLPKTGMFDFSPVVALVLLHFIRVLARNLI"
     gene            complement(4391..5191)
                     /gene="proC"
                     /locus_tag="QF838_04960"
     CDS             complement(4391..5191)
                     /gene="proC"
                     /locus_tag="QF838_04960"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HJO60067.1"
                     /translation="MTICFIGGGVMAESFIAGVLKEGLFSSDQIYVSDPITDRREYLT
                     DTYGVNCLKDNIDILGKKGKILLAVKPQTLPEVYKDLKGKISPGRPVISIVAGSNSSD
                     LSKGLEHRQIIRVMPNTPSQIGKGMLVWTSTNEVNSTDIKEIAAILKTLGEEYYVQDE
                     KYIDMATALSASGPAYVFLFIQSLIDSGVYLGMPRDMAKHLVLQTVLGSTELVLESGK
                     HPSVLSDMVTSPGGTTIEALVSMENDGLRAALINGVKAAFDRSIELGN"
     gene            complement(5211..6227)
                     /gene="pdxA"
                     /locus_tag="QF838_04965"
     CDS             complement(5211..6227)
                     /gene="pdxA"
                     /locus_tag="QF838_04965"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HJO60068.1"
                     /translation="MNNRPLLAITMGDSAGIGPEVVAKTLIDSSIYEKCNPFVIGNTE
                     AMDQAFRLINSDVSIRRVSSVEEINTNAKTVDVLDLDNLDFEKIGYGQISAESGKASV
                     EWILKAGELAASGQIQAISTAPINKEACSLAGHKDIGHMEIFQSQTGVSQVATMLMTG
                     TLRVVHLTTHRSLRIACDYVTRDNVLAKISLTHDYFQKWGFPNPRIGVSALNPHASDG
                     GLLGDEEHMQIGPAVEDACSMGIDATGPIPADTIFNQNIDGKYDVCIAMYHDQGHIPI
                     KVHDWQRSVSVNLGLPFIRTSVDHGTAFDIAGKGIADHISMMESINTAVSLVSNSELP
                     GSQK"
     gene            complement(6362..8848)
                     /locus_tag="QF838_04970"
     CDS             complement(6362..8848)
                     /locus_tag="QF838_04970"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HJO60069.1"
                     /translation="MTQGVVSGGSSNGGDRGEIREDVVKALESVGVSGEVAAALTNTI
                     IESGGIETLDENVQNDGLPLSDNARFIIEKRYLRRDDDGSPIEDPDGLFRRVSNAVAL
                     GEPEVNQAEYEEKYYEIMSNLKFLPNSPTLVNAGTGRGCLSACFVVSPEDNIQSIMKV
                     ANDAAMIEKWGGGIGFGLSDLRPKQDRISTTHGQACGPIAVMKLFSAVGTTLTQGAFR
                     LGAHMGQLIISHPDIREFIHCKDDDDTLQNFNISVQITDDFMKAVENDEDWNLINPRD
                     TGDGPSNEILGTVKAQDLWNEIAESAWKTGDPGVVFADRVWETAPNPQMGRIKTSNPC
                     GEEFLENYGNCCLGSINLDAHITGNDFDWKSLEETTRTGVRFLNDVIEVNSFPLPVLR
                     EVNLDTRRIGLGVMGWADALVRMGIPYDSEEALGLADKLGGFLNRTAWDESAKVAEER
                     GPFPEYENSALKGWGMPPVRNASVITIAPTGTISRIAGCSSGIEPHFALAWWSNVLWE
                     DHEGTSSRLLDSPSSIVESLKSAIGTEESAKEVLKQIVENPENAEGIMGQHGLDSAVY
                     RTAMNVSAEAHVKMQSVWQKHVTNSVSKTINLPNTATVQDVKDAYWLAWETGCKAVTV
                     YRDGSKSMQVLETGKTKSEGEENQEDHLKVPRQRPMSVTGVTDRVRTGHGTMFVNITF
                     DDKGHPFEVFANLGKSGSSDSAYLEAIARLSSMALRAGIDPSQIVDQLRGITDVPVWD
                     GGTLVRSAPDAVALALSRHLTNPNDGTNVIDTVASSAQLGLFPSTNKESDQQDNSNAA
                     SGAKCPECSGYLLHQEGCLSCPDCGYNKCE"
     gene            complement(8960..9514)
                     /gene="nrdR"
                     /locus_tag="QF838_04975"
     CDS             complement(8960..9514)
                     /gene="nrdR"
                     /locus_tag="QF838_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HJO60070.1"
                     /translation="MYCPFCSNESSRVIDSRDSDESIRRRRECLTCSLRFTTYERIQT
                     RALIVVKRDGRREEFNRDKLWSSMKNACAKRPLPIGSIEKAIDEIETYLVSAGRAEMP
                     SRVIGELVMDRLRNLDRVAYIRFASVYRDFRDIESFKEEVDALLQPKKEEASSQLSFL
                     KDDELTPVGRRKRGRKPKNPTAQI"
     gene            complement(9822..10892)
                     /gene="ftsZ"
                     /locus_tag="QF838_04980"
     CDS             complement(9822..10892)
                     /gene="ftsZ"
                     /locus_tag="QF838_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HJO60071.1"
                     /translation="MTISGRELEGVARIKVIGVGGGGSNAVSRMFRERISGVEYIVMN
                     TDIQALLKSDVPNRIRIGEQLTQGMGVGGDPDKGSASAEESREEIYDVIRDSNMVFIA
                     AGMGGGTGTGAAPVIAEIAQETGALTVGVVTRPFAFEGIRRSNQAQIGIERLQTNVDT
                     LLVIPNQRLSIISQEEVSAENAFRLADDVLRLGVQSITELITNPGEINLDFADVQSIM
                     RDAGPAWMSIGHASGPTRSRDAAQEAITNPLMDVSIEGATGVLFNITGGNDLKLSELH
                     EAAEVIQRVVDPEANIIFGMTTDHKMQNEIKITIIATGFPTTDTLLQRDMARTVDAVN
                     KVEINEDSLDLPPFLRRGGGNV"
     gene            complement(10928..12184)
                     /gene="ftsA"
                     /locus_tag="QF838_04985"
     CDS             complement(10928..12184)
                     /gene="ftsA"
                     /locus_tag="QF838_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HJO60072.1"
                     /translation="MNQRSTGTNSELILAIDVGSTKVCTILARKSQNGFPEVISHSVV
                     PSFGVTKGNVVDVNATSQAIRRTVGEIRADVGMDVKSAYVGITGSHIAYENRVDYFSD
                     IGRQGVVTLDEFAKIPSMVTDGLQIPGRKIIHSLPIHYSVDGHDGIKNPVGMHVTGLE
                     VTTHLITSDSYFADKLREAVLLSGIELKSLVVEPFASSESILTQAEKDIGSAIVDIGG
                     GTTDIALFRNGSLVYTAVIPVGGFQFTNDICLTYNVEFAEAEEAKLRYGHTNLSAVDL
                     METVSISPVGSSANIEIRRRDICQLMRERAVELIRLVDLKLQQGGLKENPNSLVYITG
                     GASQLPGFLEMAEQFIPNCQVRRGIPDFLMRMTDELKEPCYATAVGMVLHAYRSENSA
                     ERQLGIKDTIEEAGFLRRLMNVLKLG"
     gene            complement(12181..13119)
                     /gene="rsmH"
                     /locus_tag="QF838_04990"
     CDS             complement(12181..13119)
                     /gene="rsmH"
                     /locus_tag="QF838_04990"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HJO60073.1"
                     /translation="MNSKSGGTVHMPVMVSEVLKAFESTPEGWIIDGTFGLGGHSSAL
                     LERYPDRKVFGIDLDADSLAMSQGGFQKFNNRILLANGNYANMTILAQEADIGPVAGI
                     LLDLGLSSMQLETLGRGFSFRNDEMLDMRYNQTKGVPAAELVNTFDRDQLIDIFSNFG
                     EEKAARRIAKAVCQNRPILTTGKLAQIVEKTIGRTGKSRRHPATKVFQALRIAVNQEF
                     ENINKGLSSGLDLLVNNGKFVVLTYHSLEDRIVKQFFKKESLDCICSTRIPFCVCDHH
                     PRLRIVTKKVKIPTIEEVTINPRSRSAKLRIVEKIA"
     gene            13277..13798
                     /locus_tag="QF838_04995"
     CDS             13277..13798
                     /locus_tag="QF838_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HJO60074.1"
                     /translation="MTTARQDDNPHFYAGILTISDSGAIGERIDLSGRKISEMIKTAG
                     FKERLKDIIPDEVRIISEKLIDWSDGGEVDIILTSGGTGLGPRDVTPEATKTVLDMEI
                     PGIGEAMRIKTLEHTPFAILSRSTAGIRSGCLIVNLPGSPRGVEECLDVIMESLPHAL
                     EMIRGWKTHKESD"
BASE COUNT         4032 a         3254 c         2592 g         4019 t
ORIGIN      
        1 gccatacctc cgttgtttaa aacaattgtt gttattggtg cacctgctcg aacagaagtt
       61 tctaagtcca agcctgacat accaaaggct ccatccccca taatattcat gcagaattta
      121 tctgggcatg ccatttttac tccagtcatt agcggaattc cataccctaa gtgagtggtt
      181 ttaccccacc caacgtaact atgaggcact gtaccggggt aaaaaggcat tattatgtct
      241 cttggagctc cggcatcatg ggtcactata ctattttctt tatcaagaac gttaatcatt
      301 tctccaatga ctctatatgg ggtgatagga gtctcattcg agttgagaac cccgctccat
      361 tctgccatcc atttatcgtt tatttcagca atttgatctg acaattctga agagcctttc
      421 ctctcggccc ctgcaagttg aatcttgact tcatctacca tcatgctgag agttagcttt
      481 gtatccccga ccaacccaac atcaatattg aaatccttgt tcaaatcggc cacggtctca
      541 gtgttatgta tcatcacttt cccatctggt attggttgcc catagccagt ccgcgtaaga
      601 ctagtcccta cacccaataa aacatctgct tcctgaagcc atgtcctggc ctgtaaggaa
      661 gtcgcggagc ttccggagcc aagagccaac gggtgtcttt ggtcgaaccc ggatttacca
      721 ggcatcgtgc agtagactgg gatttgagca atttcagcaa attccgtaag ctcaggggat
      781 gcacctgaca tcaaaacacc cattcctgac cagatcacag gttttttcgc tgcgagcaat
      841 aatcttaccg cctctttaat ctctgctgaa tctggagcaa atttatgtct tttaggagga
      901 gagtaagaag aaacaactga atcatctacc tccatctcac caacgtctgc tgggatttcg
      961 acaataacag ggccagacct accgtttctt aaagcatgaa aagccctccg cataatagat
     1021 gctacctggc caggttgaaa tataacttcg gcagacactg aaactgattc ataagttcta
     1081 gctggggaaa aattcggctt gaccgatcgg gccgacactg ctgggccacc aggaagatat
     1141 agaattggta cattatccgc atatgcctga gccaaacctc ccatcgtgtt ctccgatcct
     1201 ggtccccctt gggttatcaa aactccaaac tcttctcgat ttctcatacg gctaaaccca
     1261 tccaccgcca ttgctgctcc tcgttcttga cggaacatga ttggattgat tccaacttca
     1321 gccactgcct cgatcaggtt attggatgga aaacatcctg cccattcaat tccttcggct
     1381 tttaatatcc ttgctacagc atcaagcact ttcatgaata tgattcctcc acttagaata
     1441 actatttatc tctggcaatc ggtaagcatg gcaggttcag acaacgcact actcacaagc
     1501 cgctcaaccc acattgtaat tggcataaat atcattatca acctgagtat actagtcaac
     1561 attattcgtt acgttaaact tgattcaagt gaaatttggc caaggttcac ctctattaat
     1621 caacagtgaa aaaagaaaat gcaaattgga caaaaaatac accatgtact agaaataaat
     1681 tctactatgg aagcctgtaa acggttagct aatttagggg agccaaacgg aaccatagtg
     1741 tcggcaaatt accaagaatc cggcagaggt cggttcaaga gaaaatgggt atctccaagt
     1801 ggggacaaca tccaaatgtc ggtactgctt agacccaacc agcaggaact aaaatatctc
     1861 aatattttcg catcgatggc agtcttagct acatgcgaac aaacactagg agttgatggc
     1921 agtattaaat ggcctaatga tgtccaaatc aacggtaaga aaatcgcggg aatattagtg
     1981 gaaacagtgc tagaaggtga aaaagtagta tcttgcgtaa ttggaattgg gctaaacgtc
     2041 aatctagacg taaaacccca cgctgaaatt gaagagacag caacttcact taaatgtgag
     2101 aagggccggt atataagccg ttccgtaatt ctaaaagagc ttataaaaca attagatttt
     2161 tattactcgc aaattagtga cggtaggtct ttgactcaaa gatggtgtga caaattgagt
     2221 acgataggga aaaaggtaac cttatcattt gggaaaaacc cgaataacca tatcttagtt
     2281 gggttggccg agtctatcag tgatgacggt ggcctaatta tacggaaaga ggacggttcg
     2341 cttttcacag ctaatgctgg tgaggtaact ttagcaagaa aagaggaaat attgtgataa
     2401 attggtcttt aataattatg gagtatagaa atgcttgaaa aacttaattt agaagacaaa
     2461 acaattgtca taacgggagg aggtaccggt ttaggtaagg aaatgtgctt ggccatggcc
     2521 cgagctggag ctaacctggt aattgcagca agaagacttg aaccgattga agaagtatca
     2581 aaactagtcc gaggtttggg acgtagatca ctagcaattc caactgacgc aacaaacacc
     2641 actgacatca aaacactgtt caataccgta ttggcagaat tcgggaaagt tgatgtcctg
     2701 atcaacaatg ctggaatcgt gagagaagat tcagctaggc cgatttggga aataaccgac
     2761 gaaagttgga agattggaat tgatgtgaat ctctctacag ctttttattg ttccagagga
     2821 atatcgaaac atatggcaga caatggtggt ggaaaaattg tgcacgtggc atcaggtttt
     2881 gggtttagag gtggtcggga caactacatg tatgctgctg gaaaaggggg aattgtaaat
     2941 ttaactagag ccatggcaac tagcttaggc aagtatggga ttactacaaa ttgcatagtc
     3001 cctggattta tacccacaga ggtcacagac ccaaatagcg atagatctca acagcgtgga
     3061 agatttatac caattggcag ggttgggcaa ccttcggaaa tgggtccttt ggcggtctat
     3121 ttggcctctg ccgcttcgga ttacatgaat ggggagtttt tcgctattga tggtggtggc
     3181 ctagcggggg gtatagctcc cacacttcac gcaccagaaa tacctttgga aatttaattt
     3241 tgatacagcc aacaccgcta attcaaggag caaatctact atgcgtacgc cagaaggatg
     3301 ggatctaaca ggtaaaaaag ctgtcataag cgccgatcgt agaggttgga ctaagttttt
     3361 ttccagtgcc ttagcagaag caggcgccga tgtcgcaata atcgggtcag aaaattctga
     3421 tatggaagaa gccagcaata aggcatctga ctatggccat acggtgatca cccaaacaac
     3481 taatctaatg tccaaagcct ccattgataa atccattcaa accataaatg atgccttcgg
     3541 aacaatagac attctggtga ataacgccaa agctaatttt gggaaaaagt ttgaaaaggt
     3601 aacagaatca gaatttgaca ccctaataga ttttaatctc aaatccatgt tcctaacttg
     3661 ccaatcagta gggcaaacta tgttaaataa aaaaagcggt cgcataataa atatgacctc
     3721 tgacctagct gtgaggggtt tgtggaactc tgtggttccg tgcgcttctg aaggagcaat
     3781 tcaccaatta acctcctcac tggcactgga gtggggacgg gatggaataa gggtcaatgg
     3841 tatcggcgca ggatggctta acaccgagca agaatcagat caaagtgaac caggtttatt
     3901 agagcgttat ttaccctcga aaaggaaagg gcatccaaac gaccttacac aaatgttggt
     3961 ctacctttct tcagattcat gcgatttcgt gaatggtcaa acgatattca tcgacggggg
     4021 tgcccttgcc catgcttagg ttatcacgag cgattgattc gcccaaaagg cctcaaaaca
     4081 cctgaatttg tagaatccat aatagaagct aattcaattg ggtattaaat taaatttcgg
     4141 gcgagcactc ttataaaatg cagtaatacc agagcaacca ctggtgaaaa atcaaacatc
     4201 ccagttttag gcaatacttt tcttatcggc cccatgatag gttcagtgat agcataaatc
     4261 agccgaatca ccggaaaaaa cggactacca ggggacacat taaaccaaga taccaaaacc
     4321 cgagccaata ttgaaaaata aagtactgat gcgagaatgt ttaggaaatt tgctaaataa
     4381 agtcccaaac ttaattacct aactcaattg agcgatcaaa tgcagccttg actccgttga
     4441 tcaaagcagc tctcaaacca tcattttcca tagaaactaa agcttctata gtggtcccac
     4501 caggtgaagt aaccatatca ctgagaacag aagggtgctt accgctttcc aataccaatt
     4561 cagtgctacc caataccgtc tgcagtacta aatgtttagc catatctcta ggcataccta
     4621 aatatacccc cgaatcaatc aaagattgaa taaataaaaa tacatacgcg ggaccactgg
     4681 cacttagagc agtagccata tcgatgtatt tttcatcctg aacataatac tcttcgccta
     4741 aggtttttag gattgccgcg atctctttga tatcagtgga attaacttca tttgttgaag
     4801 tccataccaa catacctttg cctatctgag aaggtgtgtt aggcatcact ctaattattt
     4861 gtcggtgttc tagtcctttc gaaagatccg aggaattact cccagccaca atagatataa
     4921 ctgggcgacc aggggatatt tttcctttga gatccttgta aacctctggt aatgtttgcg
     4981 gttttaccgc caataatatt tttccctttt tccccagtat atcaatgtta tcttttaggc
     5041 agtttactcc ataagtgtcg gttaaatatt ccctcctgtc agtaataggg tcacttacat
     5101 atatctggtc agaagaaaac aatccttctt tcagtacacc agctataaag gactccgcca
     5161 taactccacc gccaataaaa catatagtca taattcatcc ctcgagttaa tcatttttga
     5221 ctaccaggca attcactatt acttaccaaa gatacagccg tattgattga ttccatcata
     5281 cttatatggt ctgctattcc tttaccagct atatcaaatg ccgtgccatg atctacagag
     5341 gtcctaataa aaggcaatcc aagatttacg ctcacacttc tttgccagtc atgtacctta
     5401 attggaatat gaccttgatc atggtacata gcaatgcata cgtcatattt accatcgata
     5461 ttctgattga aaattgtatc agctggtata ggtccggtag cgtcaattcc cattgaacaa
     5521 gcatcttcga cagccggtcc aatctgcata tgctcttcat cacctaacag cccaccatca
     5581 cttgcgtgcg gatttaaagc agaaacccct attctaggat ttggaaagcc ccatttctga
     5641 aaatagtcat gagtcagaga tatttttgcc aaaacattat cacgagttac gtaatcacaa
     5701 gctatcctga gtgatctatg ggtggtcaaa tgaactacgc gcaaggtacc tgtcatgagc
     5761 atagtggcaa cttggcttac tcctgtctgg ctttgaaaaa tttccatgtg tcctatatct
     5821 ttgtggcctg ccaaactaca ggcttcttta ttgataggtg ctgtacttat agcctgtatc
     5881 tggcctgaag ccgctaattc accggctttg agtatccact ccacagaagc tttaccagac
     5941 tcagctgaaa tctgtccata tcctattttt tcgaaatcta aattgtctag gtctaaaaca
     6001 tcgacagttt ttgcattcgt attgatctct tcgacagatg aaactcgcct tatggaaaca
     6061 tcggaattta ttaacctaaa tgcctgatcc atagcttccg tatttcctat aacaaaaggg
     6121 ttacattttt cgtaaatact ggaatctatt aaagtcttgg caactacttc aggtccaata
     6181 ccagccgagt cccccatagt aatagcaaga agtggtctgt tattcattgg ttaatatcct
     6241 atttatttcc caaagcagga tgatatacat attgcgacca aataaagcct ataaatcaat
     6301 ctgcttccaa cttaaatgct agaaacgtac gactgaaatt ctatattgtt ctaaaaaagc
     6361 cctattcgca tttgttgtat ccacaatcag ggcaacttaa gcagccttct tggtgaagta
     6421 aatatccaga gcattccggg catttagcac cactagcagc atttgaatta tcttgctggt
     6481 cactctcctt gtttgtggag ggaaataatc ccaactgcgc tgagctggca acggtgtcaa
     6541 tcacattggt tccatcattc gggttagtta gatgtctgct taaagccaaa gcaacggcat
     6601 ctggggcaga tctcacaagg gtgcctccat cccaaacagg aacatctgta atgcctctca
     6661 actgatccac tatttgtgag ggatcaatgc cagctctcaa ggccattgag gacagccttg
     6721 cgattgcctc caaataagca gaatcactac ttcctgattt acctaagttt gcaaatactt
     6781 caaatggatg acctttgtcg tcaaatgtta tattgacaaa catagtaccg tggcctgttc
     6841 ttacacggtc agtaacccca gttaccgaca ttggtcgctg ccttggaact tttaggtgat
     6901 cttcctgatt ttcttctccc tcagatttgg ttttgcctgt ttctagaacc tgcatcgatt
     6961 tagatccatc tctatacaca gttactgctt tacatcctgt ttcccaggct aaccagtatg
     7021 cgtctttcac atcctgtaca gtggctgtat tgggtaaatt gatggtttta gacacagaat
     7081 tggttacatg tttttgccaa accgattgca tttttacatg tgcttcagcc gaaacattca
     7141 tggccgttct atacacagct gaatctaaac catgttgacc cataattcct tctgcatttt
     7201 caggattttc cactatttgc ttcaagactt ccttagcaga ttcctcagtc cctatagcgg
     7261 atttcaaact ttcaacaatt gaagacggtg aatctaaaag tctagaactt gtaccttcat
     7321 gatcttccca taacacattt gaccaccaag ccaaagcaaa atgaggttct atgccactag
     7381 agcaaccagc tattcggcta atggttccgg ttggtgcgat agttatcaca cttgcgtttc
     7441 ttactggagg cataccccat cccttcaatg ctgaattctc atattctgga aaaggccctc
     7501 tctcttcagc aactttagct gactcatccc aagcagtcct gttcaagaaa cctcctaact
     7561 tgtcagcaag acccaaagcc tcttctgagt cataaggtat ccccatccta acaagtgcat
     7621 cagcccaacc catgaccccc agaccaatcc ttcgggtgtc taaattaact tcacgcaaca
     7681 caggtaacgg aaatgaatta acttcaatta catcattaag gaaacgtacc ccggttcgag
     7741 tagtttcttc caaggatttc cagtcaaaat catttccagt tatatgcgca tcaaggttga
     7801 ttgatcctag gcaacagttt ccgtagtttt ctaaaaattc ttccccacac gggttacttg
     7861 tttttattct acccatttgg ggattagggg ctgtctccca aaccctgtct gcgaatacca
     7921 ccccaggatc tccagttttc catgcagatt cagctatttc gttccacaaa tcttgtgcct
     7981 taactgttcc taatatttca tttgagggtc cgtcacctgt gtctctggga ttaatgagat
     8041 tccaatcctc atcattctct actgctttca taaaatcgtc ggttatttga acagatatgt
     8101 tgaaattttg tagagtgtca tcatcatctt tacagtgtat gaattctctt atatcaggat
     8161 gactaataat caattggccc atgtgggcac ctagcctaaa agccccttgg gtaagagtcg
     8221 tcccaaccgc tgaaaaaagt ttcatcactg caataggtcc gcacgcctgt ccgtgagtgg
     8281 tggatatccg atcttgcttt ggccgtaagt ctgataatcc aaatccaatg cctcctcccc
     8341 atttttcgat catagctgca tcattggcaa ctttcataat agattgaata ttatcttctg
     8401 gggaaacaac gaagcaggct gataagcaac ccctaccggt acctgcattc accaaagttg
     8461 gcgaattagg aaggaatttc aggttggaca ttatttcata atacttttcc tcatactcgg
     8521 cctggttgac ctctggctcc ccgagagcca ccgcattgga cacccttcta aaaagtccat
     8581 ctggatcctc aattgggctt ccatcatcat ctcttctgag gtatcgcttt tcaataataa
     8641 acctggcgtt atctgacaaa ggcaatccat cgttttggac attttcatcc aatgtttcta
     8701 tgccacctga ttctattatt gtgttagtca gtgcagccgc cacctcacca gaaaccccaa
     8761 ctgactctaa ggcttttaca acgtcttctc taatttctcc cctatcaccg ccgttagaag
     8821 accctcccga aactactcct tgagtcatta atactactcc tcaatttaaa ctgtgatttg
     8881 aaatctcttt tcgatatcta ttattttatt tgccgaaatt agaacatttg tacgtattat
     8941 acatcgtcct gttttacttt taaatttggg ctgtcggatt tttaggtttc cttcctcttt
     9001 ttcttcgtcc gactggggtc agttcatcat ctttaagaaa tgacaattgg ctactagcct
     9061 cttctttctt gggttgcaac aatgcatcaa cttcctcttt aaaactttct atatccctaa
     9121 aatctctgta aacactagca aaccttatgt aggctaccct atcaagatta cggagacgat
     9181 ccatcactaa ctcacctatt accctcgagg gcatttctgc tcgtcctgca ctgaccaaat
     9241 atgtttcaat ctcatctata gctttttcaa tgctgccaat cggcagtggc cttttagcac
     9301 aagcattttt catactcgac catagcttat ctctattgaa ttcttctctc ctaccatctc
     9361 ttttaaccac gataagggct ctggtttgta tcctttcata tgttgtgaac ctaaggctac
     9421 aagtcaagca ctccctcctc ctcctgatac tctcatctga atccctagaa tctatcactc
     9481 tagaactctc attactacaa aaaggacaat acataaatta agccgactcc agcactcaaa
     9541 aacacagaaa gcatcactgg taaacataac aaaacacacc acaaatcgtg ttactgatgt
     9601 gaggttacgc tatatattga ggtttggggg ggaaagtctc aaggggggaa gatcattatt
     9661 ttttagagat ttttatacaa gacaggtaag ctcaggcacg aatggaaaat ttaggtcttc
     9721 tttgaacaaa atttcacaaa ataaggtttt aacgatggtg gttccggtca gcgcagtacc
     9781 ttagataacc agatttgcta tcgagttata ttaaccgcgg atcacacatt tcccccacct
     9841 ctcctcaaaa atggaggaag atctagggag tcttcgttta tttctacttt attaacggca
     9901 tctaccgttc tagccatgtc tcgttgaagc aaagtgtcag tggtaggaaa acctgtcgct
     9961 ataatagtaa tcttgatttc attttgcatt ttgtggtccg tcgtcatacc aaatattatg
    10021 ttcgcttcag gatccactac cctctgaata acctccgccg cctcatgaag ttctgagagt
    10081 tttagatcat taccaccggt tatattgaac aaaactccag tagccccttc tattgaaaca
    10141 tccatcaggg gattagtgat ggcctcttgg gctgcatcac gagatcttgt aggtccagaa
    10201 gcatgcccga ttgacatcca cgctggtcca gcatctctca ttatgctctg cacatcagca
    10261 aagtccaaat ttatctcgcc tgggttggta atgagttctg ttatcgactg gacccccaat
    10321 cttaaaacat catccgctaa tctgaatgcg ttctctgccg acacttcctc ttgggaaatg
    10381 atactaagtc tctggttggg tataactagt aaggtatcta cattagtttg cagtctttct
    10441 ataccgattt gagcctggtt tgatctcctt atcccctcaa atgcaaatgg cctcgtcaca
    10501 actccaaccg ttaaagcacc tgtttcctga gctatttctg ctatcacggg tgcagctcct
    10561 gttcctgttc ctcctcccat tcctgctgcg ataaaaacca tattagaatc tctaatcaca
    10621 tcgtatattt cttccctgct ctcttcagca gaggcagaac ctttatcagg atctccacca
    10681 actcccatac cttgggtcag ttgttctcct atccttatcc tgttaggaac atcgcttttt
    10741 aacaaagcct gaatatcagt attcatgaca atatattcaa cacctgaaat cctctctcta
    10801 aacattcgag agaccgcatt ggagccacca cctcctactc ctataacttt gatcctggcc
    10861 actccctcta attccctacc actgatagtc atacgtacct ccaaaaaaac aaaataccca
    10921 ttacaactta gcccaacttt aaaacattca ttaatcttct caaaaatccc gcttcttcta
    10981 tagtgtcttt aatacccaac tgcctttctg ccgaattttc agaacggtag gcatgcaata
    11041 ccatccctac cgcagttgca tagcaaggtt cttttaactc atcagtcatt ctcataagaa
    11101 aatctgggat tcctcttctc acctggcaat tcggtataaa ttgctccgcc atctcaagaa
    11161 aaccaggtag ctgcgatgcg ccacctgtaa tataaaccaa ggaattagga ttctctttca
    11221 atcccccttg ctgtaatttc aaatccacca atcgaataag ttccacagcc cgttctctca
    11281 tcaactggca tatgtccctc cttcgtattt caatattagc tgaagatcca acaggtgaaa
    11341 tcgacacagt ttccattaaa tctacggcag ataaattagt atgaccgtat cttaatttgg
    11401 cttcttctgc ctcagcgaac tccacgttat aagtcagaca aatatcgtta gtaaactgga
    11461 atcccccaac aggaatgaca gcagtgtaaa caagacttcc atttctaaac aaagcgatgt
    11521 ctgtggttcc gccgccaata tcaacaatcg ctgatccaat atctttttct gcttgagtca
    11581 aaatagattc gctgcttgca aatggctcca ctaccagtga ctttaattct atccctgaca
    11641 atagcactgc ctctctcaac ttatcagcaa aataggaatc agatgtaatt aaatgagttg
    11701 taacttccaa gccagttacg tgcatcccta ccgggttctt tatgccgtcg tgcccatcca
    11761 cactatagtg gatgggtagg gaatggataa ttttcctacc tggtatctgc aacccatcag
    11821 taaccattga tgggatctta gcaaattcat ctaacgtcac taccccttgt ctaccgatat
    11881 ctgaaaaata atcaaccctg ttctcatatg caatatggga cccggtgata cccacatatg
    11941 cagacttaac atccatcccg acatcagcac gaatctcacc cacagtcctc cggatggcct
    12001 ggctcgttgc attgacatcc accacattac cctttgtgac accaaaagaa gggacaacac
    12061 tatgagaaat tacttcggga aaaccgtttt gtgatttgcg agctagaatt gtacatacct
    12121 tagtactgcc aacatctatg gctaaaatta gctccgagtt agttcctgta gatctctggt
    12181 tcaagctatt ttctcaacta ttctcaactt ggcacttcta cttcgggggt taatggttac
    12241 ttcttctata gtaggaattt ttactttttt tgtaacaatt ctaagacggg gatgatgatc
    12301 gcatacacaa aaggggattc gggtcgaaca tatacaatcc aaagattctt ttttaaaaaa
    12361 ctgttttaca attctgtctt caagggagtg ataggttagt actacaaatt ttccattgtt
    12421 cacgagcaaa tcaagacctg aggacaaacc tttatttata ttttcaaatt cctggttaac
    12481 cgctattcgt aaagcctgaa aaactttcgt cgctggatgc cttcttgatt ttcccgttct
    12541 gcctatcgtt ttttccacaa tctgcgcaag cttaccagta gtcaaaatag gcctattttg
    12601 acacacagcc tttgcaatgc gtctcgcggc tttctcctcc ccaaaattcg aaaaaatatc
    12661 aattaattga tctctgtcaa aggtattgac caactcagca gcaggtaccc cctttgtttg
    12721 gttatatctc atatccaaca tttcatcatt cctaaaacta aatcctctcc ctaaagtctc
    12781 taattgcatc gatgacaacc ccaggtccaa tagtatccct gcaacaggcc ctatatctgc
    12841 ctcctgggct aaaatcgtca tgttagcgta attgccattg gctaacagaa ttcgattatt
    12901 aaatttttga aatcctccct gggacattgc aagagaatca gcatcaagat caataccaaa
    12961 aaccttccta tccgggtatc tttcaagtag agccgaagaa tgccctccta acccgaaggt
    13021 accatcaatt atccacccct ctggagtaga ttcaaaggct ttaagaacct cagaaaccat
    13081 tactggcatg tgcacggtgc cgccagattt agagttcaat ccgtagatcc cattgcccat
    13141 gttagaatgc aatgaaagca catcagctct aaatatccgc atagacctaa acaattttgg
    13201 caatttaacc gagttttttg caagggattt ttatccaaat ttacgaccat tttagcgttt
    13261 taccaaggat ataatcatga caaccgcaag gcaagacgat aacccccact tttatgccgg
    13321 tatcttaact ataagtgact caggagctat aggagagagg attgacctaa gtgggagaaa
    13381 aataagcgag atgattaaaa cagccggttt taaagagaga cttaaagata tcatacccga
    13441 tgaagttcgg attatttccg aaaaattgat tgattggtct gacggagggg aggtggacat
    13501 tatcttaaca tcaggtggaa caggactagg tccacgagac gtcacacctg aggcgacaaa
    13561 aaccgtttta gacatggaaa ttcctggtat cggggaggcg atgagaataa aaactctaga
    13621 gcacacaccg ttcgctatat tgagtaggtc aaccgctggg ataagatctg gctgtcttat
    13681 cgtaaatctg ccgggcagcc cgagaggggt ggaagaatgc ctagatgtga taatggaatc
    13741 cttaccacat gctttggaaa tgataagagg ttggaaaaca cataaagaat ccgattaacc
    13801 gattgaaccg taggaattat ttcttaaata aaaacatcat gaaattccac atttttactt
    13861 tattccctaa cctgtttgca agccctttgg aggaagg
//
LOCUS       DASCNS010000138        13872 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001134597, whole genome shotgun sequence.
ACCESSION   DASCNS010000138 DASCNS010000000
VERSION     DASCNS010000138.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001134597"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="QF838_05000"
     CDS             <1..930
                     /locus_tag="QF838_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60075.1"
                     /translation="LVGMSGPGALTTSVPPSKWFVRNRGKALAVTAVGVSLGALIFVP
                     LTQIFIDKYTWSTSWIILAIVGVIIVVPLSVIFVRRQPEDMGLLPDGDNPVNVSAVVD
                     IDDEFSWSLREAIKTKSMWFLTIAFSILSLGILTLALHRIPAFMDRGLDPTLVSLATA
                     FDAVCAGFGSFAGGMLVRRLQPKFIGASAFLMLAVASVMSIYAYGFWLMFWSMAVFGL
                     GIGINSFTQNYIWADYFGRGNLGSIRGFVMPITLFIGSFGAPAAGYVLDITGSYDPAW
                     WAGVVLMLIGALVFILADKPNHNALLLNENFKE"
     gene            1066..2190
                     /locus_tag="QF838_05005"
     CDS             1066..2190
                     /locus_tag="QF838_05005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60076.1"
                     /translation="MKFGLFFEWPNPELREFKDIFSEGLEQIKLSERLGFDYVLIAEH
                     HFSNYGFSPAPLLQALKIAENTETIKIGTAAIIVPEWQPLRAAEEIAVLDHLTEGRIF
                     CGVGRGYQPYEMGGFGIDLEESRPRFQEGLEVMTKAWREHKDWTYDGEYIKVKEPITV
                     FPKPFQKPNPPIWLAGSSEGSLKLAADQDILPLTSSMMGMEVVSRQYASYVRYRRDAG
                     KSLDDLNMALQCMTHCAPTMKEALDQLPYIRWQTRAQKALTEKKVIDGKTQGVPYEGE
                     MTDELFLDRTYLGDPDYLIEKFKKANDAGITNISLWMMWGGIEHEKLMRSIKLMGEKV
                     IPELRDLTPPESLIDNALRSDALEANSGSFTVTGEKLKDS"
     gene            2294..3331
                     /locus_tag="QF838_05010"
     CDS             2294..3331
                     /locus_tag="QF838_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HJO60077.1"
                     /translation="MNLITRQTIGVLFSTLLLLAFYACDSEGEAIYFASDRDGQWDIY
                     YSDPNAEEQINLTDSPFAERNPVLSPNRKLIAFSVSKDSKDSNVGIEVMKLKEGEKIS
                     ITTGLGIYATPRWSPDSSRLAYAAAQPGKESFVLVSDLTQSLSPRLSEVRSDQVGDWS
                     SDGNSVVFSSSNPENPGIHDRNPDGVNQRQVTCGNDVGAKWSPDNKKLAFISERSGNR
                     DIYVIDKEVEDGLCESDADSKLGLYQLTDTKGSESDISWSPDGRQILFVSERDGNSEI
                     YVMDSSGNNQVRLTQNDKTDKQPVWSPDGKNIAFVSNLDGDYDIYIMNSKGEDQKRLT
                     NNEADDLNPSW"
     gene            3427..4563
                     /gene="proB"
                     /locus_tag="QF838_05015"
     CDS             3427..4563
                     /gene="proB"
                     /locus_tag="QF838_05015"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HJO60078.1"
                     /translation="MGSKENYKYRRIIVKAGTNILTGGRDELDDSFIDNLVSQISELS
                     SSGREMLLVSSGAVAAGRGVMRNLSKSSVLPDRQVLAAVGQGRLMHLYEQAFATHGIP
                     TAQALLSRRDVTDRLGYLNLRNTLLSLLNNGVVPVINENDVVAVEELEGEVFGDNDTL
                     SALVANLIDADLLVILGSVEGMYTKDPNIFTDAELISVVEKMDGKLNEYAGPSSDLRG
                     RGGMVTKIEAARLATASGVDVVIANGTTDSVVFRLSNGERIGTLFKTSVSKLESRKRW
                     MLSGVSNKGNIKVDKGAVSALKSSKTSLLPAGVLSCFGRFERGDIVYIKDEHENNIAA
                     GISNYGSSDLLQIQSLKSDRIEQVLGHYYGDEVIHRDNMVMLND"
     gene            4587..5849
                     /locus_tag="QF838_05020"
     CDS             4587..5849
                     /locus_tag="QF838_05020"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HJO60079.1"
                     /translation="MDSLENLGKAAKTVSRRLASSSGGRRNLAIQKIATALIDSEQEI
                     ISENSKDISNGRNKGLSEAMLDRLIITKKSIHGMSSDALKVANLDDPVGEMIEMKTME
                     NGLQVGRKRVPLGVIGIIYESRPNVTVDISVLCLKSGNAVILRGGSEAFYSNQILAKV
                     IRDAISKADLPKDSIQIVQSTDRKLVSEMLKLDQYIDLMIPRGGQDLVNRVATESKMP
                     SITGGVGVCHTYVDNEASLSMAVEVVNNAKVQRPSVCNALDTVLVHSSLASDYLPRLA
                     EAFSVAGVELRCDTRALSILGPREDIKVVPAQDEDWDTEHLSLIAGVKIVDSVEEAIN
                     HIENHGTGHSDAIITENYSTADRFVREVDSSAVMVNASTRFNDGGQFGLGAEVAISTN
                     KMHARGPMGLKEITSYKWVVLGTGHIRE"
     gene            5932..6882
                     /locus_tag="QF838_05025"
     CDS             5932..6882
                     /locus_tag="QF838_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60080.1"
                     /translation="MQDENIFYTVDMQREVRDFVQNLEKELAEYPPVYERDPVEIRND
                     QEEGLGRYEKPILSQKAIEREIQSDQGPVHVRAFIPDDQIDGVYLHIHGGGWVFGRAH
                     HRDGDLEEIMNDCNAAVISVDYRLAPENPYPAAQDDCEAVALWVVDNAMEEFGTDKIA
                     IGGESAGGHLSASTMIRMRDKHGFTGFTGANLVYGVFDMSGTPSLRLWGDRNLVLSTP
                     TMNWFFDHYLPNQDRRDPDVSPLYAHLHELAPALFTVGTLDPLLDDTLFMHSRWFASG
                     NPSTLNVYPGATHGFERQGTLLGEKVRARMRTFISESFQS"
     gene            complement(6857..8122)
                     /locus_tag="QF838_05030"
     CDS             complement(6857..8122)
                     /locus_tag="QF838_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60081.1"
                     /translation="MKNLAKSIYRWHPSPPFFYGWIVLGTASIGALIATSVAQTVFGG
                     VQDLIAGEIGWDRRTIALAATLGTWASGLTMPFIGRLVDKFGPRWMMFFASLLVGFGI
                     IYFSSSQSLWQFFTAYIVVRAIAGPNLQNLIPRTVAVNFFEKKRNFAMGITALNRIIG
                     ESINIQIITGIATAYSWRTAYKVIGLVSIPLSLPILLLMRHKPEDVGQLPDGAVNTTL
                     GKADPPISEQTWTVSRIMSLPSFWFIMIGEFVAVTSTSMTLFQVVPFLTDGGISQSTA
                     AGALTLGNVLGGLSVPVWGYLTDRFTTKKIAICVLVLAILPSILFTVIDIPPYGFALV
                     VFWTTITSLIFVLGSMMLGTVFNRASFGTVTGLTGPTRTAAMGLGPSVGAFAVAWIDS
                     YMPIFLATAIGYFCVIFLYYNVRTENSRR"
     gene            8282..9109
                     /locus_tag="QF838_05035"
     CDS             8282..9109
                     /locus_tag="QF838_05035"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HJO60082.1"
                     /translation="MAYEFIKYEASGGIARVILNRPEKLNALSQQLQSELIECLTNAD
                     EDPDIRVITLRGAGRAFCAGYDITPPQSDEDREITDARRGNIRSDMQRLQKTARLMTS
                     IFDLSKPVIAGIHGHVIAGGTDLALHCDIIIAAEDANIGFPPVRSMGTPPTHMWTYMV
                     GPQWAKWFMLTGETISGKEASDIGLVWKSVPAEGLDATVEDLATKMAKIPWEMLAANK
                     SIVNKALDLMGRNMMQVIAAETDAVSHQSPIVKEFNEISSQQGLKAALEWRDSPFRE"
     gene            9130..9915
                     /locus_tag="QF838_05040"
     CDS             9130..9915
                     /locus_tag="QF838_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60083.1"
                     /translation="METKTQQAADQLYREYSQKTPYSLMNTDLAPISEAAAYDIQKEF
                     MFKRAQNSGGYSGYKIAYTTKVMQERVGASQPVFGRILSNSVYQSPASLFAVNYVNLA
                     LECEVAVTMASDLEAKDKGVDRDTVFDAIEHLQLAFEVVDIRKSASENSLIQSIATNI
                     HGAGVVLGQPVTKWRELDIPSAKCELKLDGVSVGTGKGSDVDGHPVEPMIWIAKTLNA
                     MGGSLKKGDIVITGSMIPPIEVKPGMKAALQMDLFGEITLDVK"
     gene            9963..10805
                     /locus_tag="QF838_05045"
     CDS             9963..10805
                     /locus_tag="QF838_05045"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO60084.1"
                     /translation="MFDLKDRVAIVTGGAQGVGGGISRMFAKAGAKVLIADLDLETAE
                     DTANQISNSSGIVECVQTDVTSYEDLKKMINTARDHWGKLDILVNNAYAAADGQQGGA
                     LEVTEERWEKDIRALVTSVYAGSKYAIPLMLETGSGSIVNISSVHGFLVARKALTYET
                     GKAAVIAMTKQMAVDYGRQGIRVNAICPGHIVTERIKESVWKDNPTGLKFFEDQYPVG
                     RTGVPEDIGNAATFLCSDEASFITGHALPVDGGLTIQLQENFGVQQVQYYMDNVDTQM
                     PYKR"
     gene            complement(10848..12011)
                     /locus_tag="QF838_05050"
     CDS             complement(10848..12011)
                     /locus_tag="QF838_05050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO60085.1"
                     /translation="MTYPELTYGPITREEAKPLWETIYQSLHRHAQPGWNEHVGYEQF
                     RAVKSKNKILGGMGILDLGQWFGGNIVRTGAVTSVGVAPEGRGIGAASVLLRNSLSEM
                     YELAIPISTLFPSSTRVYRSFGYERSGTKFTYETTVKEMKAPLNELEVLESNPSEREE
                     TYLLYNERAELSNGNLDRGSVLWDLILETQGRKIFHYVVMDDDKAVGYVNFQQARSAD
                     HIRVRDMVALNSKCAERLLRFFYDHRTVIDTISWNGPPNDPMRLLMEEQEVKIAYSRD
                     WMLRIIDIKKAIESRGYPKDIETTLTLKYEDPILPQNSGTWTIEVSEGKGLVSKSNLP
                     GLTLGPRGLAPLYTSYLSAFDIKNMGLIEGSSDELAKASLIFSGPKPWIGDQF"
     gene            complement(12036..13133)
                     /locus_tag="QF838_05055"
     CDS             complement(12036..13133)
                     /locus_tag="QF838_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60086.1"
                     /translation="MKITDMRIESYRWPKPTPQSNGKHTYTHDGRNFVFIDTDEGITG
                     VGLIGGLHTSDSISKAIFEHYKESVIGEDPFCNEKIWDDLWEPKISGRRGMTTRVISG
                     IDIALWDIKGKAANQPVYRLLGGYTKKVPVYIAGGYYEDGKGLKELQEEMLTSVGMGA
                     NAVKMKIGAVSINEDVERVKAVRVAIGPNVKLMVDANCAYRFYEAIEIAKKMEPYDIF
                     WFEEPIAPDDYKGHQLISQSTTIPIATGENEYTKYGFRDLIESRSAAILQPDALIMGG
                     VTEFMKVSAQAQAHNLPIAPHGKQEVHIHLLCAIPNGLILEYYREETDPIFNQIYEFT
                     LQVEDGYVSPPDLPGFGINPNMDILKQYRVS"
     gene            13263..>13872
                     /locus_tag="QF838_05060"
     CDS             13263..>13872
                     /locus_tag="QF838_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60087.1"
                     /translation="MNFSKTHLYSKKIEAQSAFESGDSAFEVLAEQILEIGDVNDAMR
                     NLVQEGFTGQDDRPVGGLKDIVSRSQEERRFLLEKYTYDKILPGDLLEELQNYPFLGS
                     ESLTIEKGNDILSMLKQLDKLDEAIKEAMKTGQLGNLDLDELEDLLGKLARTQLEEIL
                     RMISALEEAGYLLKNRDRFKLGPKAVREIAQDAMREIFSQMKK"
BASE COUNT         4042 a         2532 c         3318 g         3980 t
ORIGIN      
        1 ttggtcggta tgagtgggcc cggtgctctt actacatctg tgccgccatc caagtggttt
       61 gttcgaaaca gaggaaaagc tttagccgtt acagcagtag gtgtttcctt aggggcccta
      121 atttttgttc cgctcaccca aattttcata gataaataca cttggagcac ctcttggatt
      181 atattagcga tagttggagt catcattgtt gttcccctct ctgttatttt tgtcagaaga
      241 caacctgaag acatgggatt gcttcctgat ggtgacaatc ctgtcaatgt gtctgccgtg
      301 gtggacattg acgatgaatt ttcgtggagc ttaagggaag ccataaaaac aaaaagcatg
      361 tggtttctta caatagcttt ttctatcctg tctctgggaa tactgacact ggccctgcat
      421 aggatacccg ctttcatgga tagagggctg gatcccacat tagtctcgct tgctacagct
      481 tttgatgctg tttgtgccgg gtttggtagt tttgcagggg gaatgttagt aaggcgcctt
      541 cagcctaaat ttataggtgc ctcagcattc ttgatgttgg cggtggcgag tgtgatgtca
      601 atctatgcgt atgggttttg gctgatgttt tggtctatgg ctgtttttgg gcttggaata
      661 ggcattaatt cattcacaca aaattatatc tgggcggatt attttggaag gggcaacctg
      721 ggtagcattc gaggatttgt tatgcctatc actttgttta taggatcttt tggtgctcca
      781 gctgcaggct atgtcttgga tatcacggga tcatacgatc ctgcctggtg ggctggtgtt
      841 gtcttgatgt tgataggagc tttagtattt attcttgcgg ataaacccaa ccataatgca
      901 ctcctcctta atgaaaattt caaggaatga attgccaatt ggaagcattg aagatttgag
      961 ctagagatgg gttcaaaatc ctagaatatt cttggagtat taggatattc tgttaatgtg
     1021 ggtgagaggt tcaccgtaaa gaatttaaca aggaagtgaa ttaaattgaa atttggctta
     1081 ttttttgaat ggcctaatcc agagcttcgt gaatttaaag atatattttc agaaggattg
     1141 gaacaaataa aattatctga gaggttaggt ttcgactatg tgcttatagc tgaacatcat
     1201 ttttccaatt atgggttttc tccggcacct ttgttacagg cgctaaagat agccgaaaat
     1261 acggaaacta tcaaaatagg tactgcagct atcattgtgc cggagtggca gccacttagg
     1321 gctgctgaag aaatagctgt tttagatcat cttaccgaag gccggatatt ttgtggggtc
     1381 ggtagagggt accaacctta tgaaatgggc ggatttggaa tagaccttga agagtcccgg
     1441 cctaggtttc aggaagggtt agaggtcatg accaaagctt ggcgagaaca caaggattgg
     1501 acctatgatg gagaatacat aaaggtaaaa gaaccaataa cagttttccc aaagcccttt
     1561 caaaaaccta atccacctat atggctcgct ggatcttcag aaggatcttt gaaattagca
     1621 gccgatcaag atattctgcc gttgacctct agcatgatgg gtatggaagt agtttctcga
     1681 cagtatgcta gttatgtccg atacaggcgg gatgctggta aatctttgga tgatttgaat
     1741 atggcactcc aatgtatgac ccattgtgcc ccgactatga aggaagccct tgaccaattg
     1801 ccttatattc gctggcaaac aagggcccaa aaagccctaa ccgaaaaaaa ggtaattgac
     1861 gggaaaacgc agggagttcc gtacgaaggt gagatgaccg acgaattatt tcttgaccgt
     1921 acatatttag gtgatcctga ctatctgatc gaaaaattca agaaagcgaa tgatgcagga
     1981 ataactaata taagcctttg gatgatgtgg gggggtattg aacatgaaaa attaatgcgt
     2041 tctataaaat taatgggaga aaaggtgatt cccgaactaa gagatttaac ccctcctgaa
     2101 agcctaatag ataatgcatt aagatcagat gccttggaag ctaacagtgg atctttcact
     2161 gtaacaggag aaaaactaaa ggatagttaa agcacagtca ttgctatctt tatatgaagg
     2221 gcttggaggt tttgcgccga atgtgggttt gaaacgtgta gccctataaa tatttcatcg
     2281 gtaaaatctg ggtatgaacc taataacgag acagaccatc ggggttttat tctccacact
     2341 cctattattg gcattttatg cttgtgactc tgaaggggag gctatctatt ttgcctcgga
     2401 ccgggatggg caatgggata tttattactc tgatccaaat gctgaagaac aaatcaattt
     2461 aacagatagt ccattcgcgg aaagaaatcc agttttgtca ccaaaccgaa aattgatagc
     2521 attttcggta agcaaggatt ccaaggactc gaatgtggga attgaggtaa tgaagctgaa
     2581 ggaaggagaa aaaattagta ttactacagg tcttggtatc tatgccacac cacgttggag
     2641 ccctgatagt tctaggttgg cgtatgctgc cgcccagccg ggcaaggaat catttgtgct
     2701 tgtaagcgac ttgactcaat cacttagccc gcgcctgtca gaagtacgat ccgatcaagt
     2761 tggagattgg tcttcagacg ggaattctgt tgtattttca tcttcaaatc cggagaatcc
     2821 aggtattcac gatagaaatc ctgacggcgt taatcaacgg caagtgactt gtgggaatga
     2881 tgttggggcc aaatggtcgc cagataacaa gaagttagcc tttatatctg aacggtctgg
     2941 gaatcgagat atatacgtga ttgataagga agtagaagat ggattatgtg aatccgacgc
     3001 cgattctaaa ttggggttgt atcaactaac agacacaaaa ggtagtgagt ctgatatttc
     3061 atggtctcct gatggaagac agatattatt tgtctcagaa agggatggga attcagaaat
     3121 ttatgtaatg gatagtagtg gaaacaatca agttcgattg actcaaaatg acaagacaga
     3181 caaacaacct gtttggtctc ccgacgggaa aaatattgca ttcgtatcga atttggatgg
     3241 cgactatgat atttacatca tgaatagtaa aggggaagat caaaagaggt taaccaacaa
     3301 tgaagccgat gatctcaatc ccagttggta atttccctgc tttttaacat caaatcttga
     3361 taaatgcttt ggtgacaccc tatttgggta tgtgctaaaa atggctggct gattttggac
     3421 gtaatgatgg gttctaaaga aaactacaaa tatcgacgaa ttattgtgaa ggccgggaca
     3481 aatatactta ccggcggaag ggatgaattg gatgattcct ttatcgataa cttggtttct
     3541 caaatttcgg aattatcctc ttcaggaagg gagatgcttc ttgtatcatc gggagctgtg
     3601 gcagctggaa gaggggtgat gaggaattta tcgaagagtt cagttttacc agacaggcaa
     3661 gttttggcgg cggttggtca aggcagactg atgcatttat atgagcaagc ttttgccact
     3721 catgggattc ccactgccca agccttgctt tcccgtaggg atgtgactga caggttaggc
     3781 tatttgaatt taagaaacac attactttcc ttgttgaata atggtgtagt tccagtaata
     3841 aatgaaaatg acgtagtggc tgtagaggaa ctagaagggg aagttttcgg tgataatgat
     3901 accttgtctg ctctagttgc taacttaatt gatgctgatt tactagtgat attaggtagt
     3961 gtagagggga tgtacactaa agacccaaat atttttacag acgctgaact tatatcagtg
     4021 gtagaaaaaa tggacggtaa gttaaatgaa tatgctggcc cttcgtcaga tttaagagga
     4081 cgcggcggga tggtaaccaa aatagaggct gctcgtttgg ctacggcgtc aggggttgat
     4141 gttgttattg caaatggcac cacggattcg gttgtttttc gtctttcgaa tggagagaga
     4201 ataggaactc tgtttaagac atcggtatca aaattggaaa gtaggaaaag atggatgctc
     4261 agtggggtgt ctaataaagg aaatataaaa gtagacaaag gggctgtatc ggcattgaaa
     4321 tccagcaaga caagtctcct accagctgga gtgttgagtt gttttggacg atttgaaaga
     4381 ggggatatcg tttatattaa ggatgagcat gaaaataata ttgcagctgg cattagtaac
     4441 tatgggtcct ctgatttgct gcaaatacaa agtttaaaat ctgaccgaat tgaacaggtt
     4501 ctaggacatt actatgggga tgaggtcata catagggata atatggttat gttaaatgat
     4561 tgaagttcca aagagggggt gttggcttgg atagtctcga aaatttaggc aaagctgcca
     4621 aaacggtttc tagaagactt gcttcttcct ctggaggaag aaggaattta gctattcaga
     4681 agatcgcgac ggccctgatt gattccgagc aggagatcat ttcagagaat tcgaaagata
     4741 tatccaacgg ccgcaataaa ggattatcag aggccatgtt ggacagattg attattacga
     4801 agaaatcaat acatggtatg tcatctgatg ctttgaaggt tgctaattta gatgatccgg
     4861 tgggggaaat gattgaaatg aaaaccatgg agaatggtct tcaagttggg cgaaaacgtg
     4921 tgcccttggg cgtaattggc attatatatg agagcagacc aaacgttact gtcgatatat
     4981 cggtgctgtg cttgaaatct gggaatgccg taattcttag aggagggagt gaggcgtttt
     5041 attcaaacca gattttggcc aaggttatta gagatgctat atcaaaagct gatttgccaa
     5101 aagattccat tcagattgtc cagagtaccg acaggaaact ggtttctgaa atgctgaaat
     5161 tagatcagta tattgatcta atgattccaa gaggtggaca agacttagtg aacagagtgg
     5221 ctactgaatc caagatgcca tccatcactg gcggtgtagg agtttgccat acctatgtag
     5281 ataatgaagc ttcgttatct atggctgttg aagttgttaa taacgccaaa gttcagcggc
     5341 catcggtctg taatgcattg gatactgttt tggttcattc ttctttggcc tcggattatc
     5401 ttccccgttt agcggaagct ttttctgttg ccggcgtaga gttgagatgt gataccagag
     5461 ctctttctat tttggggcca agggaagata tcaaggtggt tccggcccag gatgaagatt
     5521 gggatacaga acatttgtca ttgatagcag gagtgaaaat agtagattca gtagaagaag
     5581 ctattaatca catcgaaaat catggtacag gccattcgga tgccattatt acagaaaatt
     5641 attcaacagc ggacaggttt gttagggaag tggattcaag tgctgttatg gtaaatgctt
     5701 cgactagatt taatgatggc ggccaatttg gattaggggc tgaagtggct ataagtacta
     5761 ataaaatgca tgccagaggc cctatgggac tgaaagagat aacctcgtac aaatgggttg
     5821 ttctagggac tgggcatata agggaataac agaaagcaga ttcaaaagaa taaaccaaga
     5881 aatttgaaat caccgaaggt tatgtaagtt tcgactaata acgaggacac gatgcaggac
     5941 gagaatattt tttatacagt ggatatgcaa agagaagttc gggattttgt acaaaatcta
     6001 gaaaaagagt tagctgaata cccgccggta tatgagaggg atccggtaga aatcagaaat
     6061 gatcaagaag aaggacttgg gcgctatgaa aagccaattc tatcgcaaaa agccatagag
     6121 cgagaaattc aaagcgacca gggaccagtt cacgtaagag cttttatacc cgatgatcag
     6181 atagatgggg tatatctgca tatacacggt ggcggttggg tatttggaag agcacatcat
     6241 cgggatgggg atttagaaga aataatgaat gattgcaacg cagcagttat ttctgtcgat
     6301 tatagattgg cacctgaaaa tccttaccca gcagcccaag atgattgtga agcagttgct
     6361 ctgtgggttg ttgacaatgc tatggaagaa tttggcacgg ataagattgc cattggtgga
     6421 gaatcagctg ggggacacct gtccgcttct actatgatcc gtatgcgaga taaacatgga
     6481 ttcactgggt ttacaggtgc taatttggtg tatggcgtgt ttgacatgtc tggaacccca
     6541 agtcttagat tgtgggggga tcgtaatttg gttttgagta ccccaacgat gaattggttt
     6601 tttgatcatt atttacctaa ccaagatagg agagaccctg atgtatcgcc cctgtatgcg
     6661 cacttgcatg aattagctcc ggctcttttt actgttggga ctttggatcc acttttagac
     6721 gacactttgt tcatgcattc aaggtggttt gcgtccggaa atccatctac attaaacgta
     6781 taccctggtg ctactcatgg ttttgaaaga caagggacat tgttgggtga aaaagttaga
     6841 gctaggatga ggacttttat ctccgagagt tttcagtcct gacgttgtaa taaagaaaaa
     6901 tcacacagaa atatccgatt gccgtggcta gaaaaatagg catatagcta tctatccatg
     6961 ctacagcgaa tgctccgacc gaaggtccca aacccattgc agcagttctg gttgggcctg
     7021 tcagtccggt aacagtacca aaagaggctc tattaaatac tgttcctaac atcatgctgc
     7081 ctaacacaaa tatgagacta gtaattgtgg tccaaaatac gacaagggcg aatccgtagg
     7141 gtggtatgtc aattactgta aaaagtatcg agggtaggat ggctaaaacc agaacacaaa
     7201 tcgcaatttt ttttgttgtg aatctatctg ttaaatatcc ccaaacgggt acagaaagcc
     7261 ctccgagaac gtttcctaaa gtcaatgcgc cagctgcggt gctttgggaa attcctccat
     7321 ctgttaagaa agggactact tgaaataggg tcattgaagt cgaggtaacg gctacgaatt
     7381 cacctatcat tatgaaccag aatgatggca aggacattat cctggacacg gtccaagttt
     7441 gttcagaaat cggaggatcc gctttgccta aagtagtgtt tacggcacca tccggtagct
     7501 gtccgacatc ttctggttta tgcctcatca ataataaaat aggcaatgac agtgggatgg
     7561 atactaaacc gattactttg tatgctgtcc tccatgagta ggctgtggca atgccagtta
     7621 ttatctggat attgatggat tcacctatta ttctgtttag cgcagtgata cccatagcga
     7681 aattccgctt tttctcaaag aaatttacag cgactgtgcg gggaataaga ttttgtagat
     7741 tcggaccggc tatagctcgg accacaatat acgcagtgaa aaattgccat aatgactggc
     7801 tactggaaaa gtagataatt ccgaacccaa ctagcaggga tgcaaagaac atcatccatc
     7861 tgggcccaaa tttatctacg agccttccta taaacggcat tgttaaaccg gatgcccatg
     7921 ttccaagagt ggctgctaaa gcgatagttc ttctatccca tccaatttcc cctgcaatca
     7981 aatcctgtac ccctccaaaa actgtttgag ctacagatgt agctatcaaa gcaccaattg
     8041 aggcagtccc taaaactatc cagccgtaga agaagggtgg ggaggggtgc cagcgataga
     8101 tgcttttcgc taaatttttc aagaacagcc cttatttttg tcgatttgag cgatgaacta
     8161 gtacaaaagc taattttaat acttcttcgt gataatattt cgccgcccct aatctattca
     8221 ataaaaaaga aaaatggaat tagggtcgta tctgaagaat tttagaaaag gagagatata
     8281 aatggcatac gaattcataa aatatgaagc atcgggcggg atagcaagag tgatcctgaa
     8341 tcggcctgag aaactcaatg ctctaagcca acagttacaa tctgaattga tcgagtgtct
     8401 gaccaatgct gatgaggacc cggatatacg agtcataact ctaagagggg ctgggcgggc
     8461 attttgtgca ggatacgata tcactcctcc tcaatcggat gaagatagag aaattaccga
     8521 tgctcgacga ggaaatatcc gatcagatat gcagagattg caaaaaacgg ctaggttgat
     8581 gacttcaata tttgatctga gtaagccggt tatagccggt attcatgggc atgttatagc
     8641 tggcggtacc gatttggcct tgcattgtga catcatcatt gctgctgaag acgcgaatat
     8701 tggatttccg cccgtccgct ctatggggac tcctcctacc cacatgtgga cttatatggt
     8761 tggtcctcaa tgggcaaaat ggttcatgct aaccggagaa actatctctg gtaaagaggc
     8821 gagtgacatt ggattagttt ggaaatctgt tcctgcagaa ggactagatg caacggtgga
     8881 agatttggct acaaaaatgg caaaaatacc atgggaaatg ttagcagcca acaaaagtat
     8941 agtgaataaa gcccttgatt tgatgggtcg caatatgatg caggttattg ctgctgaaac
     9001 agacgctgtt tctcaccagt ctcctattgt caaagagttt aatgaaattt ccagtcagca
     9061 aggattaaaa gctgctcttg agtggaggga tagtccgttt cgagaataat attttacctg
     9121 gagtaaaaca tggaaaccaa aacccaacaa gccgctgatc agctatacag ggagtatagt
     9181 cagaaaactc catattcttt gatgaatacc gatctggcgc ccatttcaga agctgcggca
     9241 tatgatattc aaaaggaatt catgttcaaa cgtgctcaaa attctggagg gtattccggt
     9301 tataaaatag cgtataccac taaagtgatg caggagcggg tgggcgcctc tcaaccagta
     9361 ttcggaagga tattatctaa ttctgtatat caatcaccgg cttcactctt tgctgtgaac
     9421 tacgttaact tagctttgga atgtgaagtt gcagtgacga tggcttctga cctggaagcc
     9481 aaggataagg gggtggatag agacacggta tttgacgcca ttgagcacct gcaactagct
     9541 ttcgaagttg tagatattcg aaaatcagca tcggagaatt ctttaattca gtccatagca
     9601 accaatatac acggggctgg ggtcgtgctg ggacaacctg tcactaaatg gcgggaactg
     9661 gatattccat ccgcgaaatg tgagttaaaa cttgatgggg tatccgttgg tacgggtaaa
     9721 ggatcagatg ttgatgggca tcctgtagaa ccaatgattt ggatcgcaaa aaccctaaat
     9781 gctatgggag gttctttgaa aaaaggagat atagtgatta cagggagtat gattccaccc
     9841 attgaagtta aacctggtat gaaagccgcc ttacagatgg atttatttgg agaaataact
     9901 ctggatgtta aatgagaagc attgggtgta agcaatagat gatagatata aggtggatat
     9961 aaatgtttga tttgaaagac agagttgcaa tcgtgactgg aggtgcccaa ggtgttggtg
    10021 gaggaatttc aagaatgttc gccaaggctg gtgccaaagt cttaatagcg gatctggatc
    10081 tagagacggc agaagacacc gctaatcaga tctcaaatag ttcaggcatt gtcgaatgtg
    10141 tccaaactga tgtgacttct tatgaagatt taaaaaaaat gatcaatacg gccagagacc
    10201 actgggggaa gttggatatt ttggtcaaca atgcgtatgc agcagccgac ggacagcagg
    10261 ggggagcact ggaagttaca gaagaaagat gggaaaagga tatcagagcc ttggtcactt
    10321 cagtttatgc aggttcaaaa tatgcaatac cattaatgct ggagactggg tctggatcaa
    10381 ttgtgaacat atcatcagta catgggtttt tagttgctag gaaggcatta acctatgaaa
    10441 caggcaaggc agctgttatc gctatgacta aacagatggc ggttgattat ggtcggcagg
    10501 gaattagggt gaatgcgatc tgcccaggtc atattgttac agagaggata aaagaaagtg
    10561 tttggaaaga taatcccacg ggtttaaaat tttttgaaga tcaatatcca gttggaagaa
    10621 caggggttcc ggaggatata ggtaatgctg ccactttttt atgctcagat gaggcttcat
    10681 ttatcacggg acatgccctt ccagttgatg gcgggttaac gattcagttg caggagaatt
    10741 tcggagttca gcaagtccag tattacatgg ataatgtgga tacccaaatg ccatataaaa
    10801 ggtagtatga acagatacca tctcttatcc gagtagcgga ttcaatatca gaattgatca
    10861 cctatccatg gttttggccc gctgaatatc aaagatgctt tggctagctc gtcgcttgag
    10921 ccttcaatca agcccatatt tttgatatcg aaagcactga ggtaactcgt gtataacggt
    10981 gcgagaccac gaggtcctaa agttaatcct ggtaaattag atttactgac caaacctttt
    11041 ccttcagaca cctctattgt ccaagtacct gaattctgcg gcaagatcgg atcttcatat
    11101 tttagagtca gcgttgtttc aatatccttt ggataacctc tggattcaat tgctttttta
    11161 atatctatta ttcttaacat ccagtcccta ctataggcaa ttttcacctc ctgttcttcc
    11221 atcaacagcc tcataggatc atttggaggg ccattccacg agattgtgtc aatgacagtt
    11281 ctatgatcat agaagaaacg gagtagtctt tctgcacatt tagagttcaa agcgaccata
    11341 tctcttactc ttatatggtc cgcagatcgg gcctgttgga agttaacgta gcctactgct
    11401 ttgtcatcgt ccattacgac gtaatgaaat atttttcgac cttgagtctc caaaataaga
    11461 tcccagagga ctgaacccct gtctaggttt ccattactca attccgccct ctcgttatag
    11521 agcagataag tctcttcgcg ttcggaagga tttgattcaa gaacttccaa ctcgttaagt
    11581 ggagccttca tctcttttac tgtggtttcg tatgtaaatt ttgtgcctga tcgctcatat
    11641 ccaaaagagc gatacactcg ggtactcgat gggaataaag tagaaattgg aatagctaat
    11701 tcgtacattt cggagagtga attcctaaga agcactgaag ctgctccaat gcctctgcct
    11761 tcaggagcaa ccccgacaga ggtaactgca ccagttctta ctatgtttcc accgaaccat
    11821 tgacccagat cgagtattcc cattccgccg agtattttat tttttgattt tacagctcga
    11881 aattgctcgt aacctacatg ttcattccaa ccgggctggg catgtctatg aagtgattgg
    11941 tatatggttt cccacagggg tttggcttct tctctggtta ttgggccata tgttaattct
    12001 gggtaggtca tttattgttt tccaatctgt ctagtttatg aaactctgta ttgcttcaaa
    12061 atgtccatgt taggatttat tccaaagcca ggaaggtcgg gtgggctaac atagccgtct
    12121 tcgacttgaa gggtaaactc atatatttga ttgaatatag ggtccgtttc ctccctgtaa
    12181 tattctagaa ttagaccatt gggtatcgcg cacaatagat gtatgtgaac ttcttgttta
    12241 ccatgagggg ctattggaag attgtgagcc tgtgcttgag cagatacttt cataaattcc
    12301 gttacgccac ccattattag cgcgtcgggc tgcaagattg cagcacttct actttctatt
    12361 agatctctga atccatactt tgtgtattca ttttcaccgg tagcaatagg aatggtagtc
    12421 gattggctta taagttggtg acccttgtaa tcatctggag cgatcggttc ttcaaaccaa
    12481 aatatgtcat aaggttccat tttttttgca atctcaattg cttcgtaaaa gcggtaggca
    12541 cagtttgcat ccaccattaa cttcacattt ggtccaatag ctactcgaac ggccttaact
    12601 ctttcaacat cctcgttgat actgacagca cctatcttca ttttcacagc attggcaccc
    12661 atgcccacac tagtcagcat ttcttcctgc aattccttca gtccctttcc gtcttcataa
    12721 tacccgccag caatgtatac aggaactttt tttgtgtagc ctcctaaaag cctatagaca
    12781 ggttggtttg cagctttccc ttttatgtcc cagagtgcaa tgtcaattcc gctgatgacc
    12841 ctggtagtca ttcctctacg tcctgatatc tttggttccc aaagatcatc ccaaattttc
    12901 tcattgcaaa aaggatcttc gccgattacc gattctttgt agtgttcaaa gattgctttg
    12961 ctgattgagt cagatgtatg aagacctcct atgagaccaa ctccagtgat accttcgtct
    13021 gtatcgatga aaacaaaatt tctaccgtcg tgggtataag tatgttttcc attgctttga
    13081 ggggttggtt taggccatct ataactctct attcgcatat cggtaatttt catatttaaa
    13141 ccctcgtata atgtaaggcg caaaccaatc agcaaggagc tgattctagc atgaacaatg
    13201 tagttatata tccatatggg aagctttctt ttaatgtgtg atcttcgaac tgtggagcaa
    13261 gtgtgaattt ttcaaaaacc catctatatt caaaaaaaat tgaagcacaa tctgcttttg
    13321 aaagtgggga ttcagctttt gaggtgttgg ctgaacagat attggagatt ggtgatgtca
    13381 atgatgcaat gcgtaatctt gtacaggaag gatttactgg ccaggatgat agacccgttg
    13441 gtggcttgaa agacattgtg agccggagtc aagaggaacg aaggtttcta ttagaaaaat
    13501 atacctatga caaaattctg ccgggagatc tacttgaaga gctacagaat tatccttttt
    13561 tgggttcgga atcgctgacg atagaaaagg gaaacgatat tttatcaatg ttgaaacagc
    13621 tagataaatt agatgaggcg attaaggagg ctatgaagac ggggcagctt gggaatttag
    13681 atttagatga gttagaagat ttacttggga agttggctcg tacccaactt gaggaaattc
    13741 ttcggatgat aagtgctctt gaggaggccg ggtatttatt aaaaaaccgc gataggttca
    13801 aattgggacc gaaggcagtc agagagattg cacaagatgc aatgcgagaa atattttcgc
    13861 agatgaaaaa gg
//
LOCUS       DASCNS010000139         4043 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001150138, whole genome shotgun sequence.
ACCESSION   DASCNS010000139 DASCNS010000000
VERSION     DASCNS010000139.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001150138"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="QF838_05065"
     CDS             <1..1496
                     /locus_tag="QF838_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012975.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HJO60088.1"
                     /translation="IKNQSIPPNPSDHQSQLIQHLKKFIILGESYNRSAKATDLINRI
                     SSNSQGNLQKTGFKLLEPTGVINLDIPYELEKLGIPIEFSEDLITKSETLIPLDYDWE
                     DLTHLPTYTIDDDDTLDRDDAFSIDEYNRHIWVHITAAASIIDSGSIFDLEADNRMCS
                     VYLPEMTIPMLPTPISENISTLLHGSRRLCLSLRLDLDENSNLIRHSFVKTVIETNES
                     LTYTVADNIFNDSTEELHDVLSKLEEFTTKSRSERERQGAHSFNKPGMTIKISKDGKI
                     KVSMSAVQSRSKDLIAELMIVYNAKISAFFMTNQIPALYRSQDDSTNSHAMAGNQPLD
                     WYLAARSMKPAKVSVSPSRHSGLGLDLYCQVTSPLRRFSDLCLQRQAIEYISSGTNTY
                     GPKELSTIGFKSEVRIKEIHRLESRRKKYWLLRFLEQETLNGTVSNRLSATVLENGPL
                     RSALIELTEYPLRGRCLIPQEYNPGDECELKLNGVDLWDLTAQFSLL"
     gene            complement(1517..1591)
                     /locus_tag="QF838_05070"
     tRNA            complement(1517..1591)
                     /locus_tag="QF838_05070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1557..1559),aa:Pro,seq:tgg)
     gene            1767..2633
                     /locus_tag="QF838_05075"
     CDS             1767..2633
                     /locus_tag="QF838_05075"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO60089.1"
                     /translation="MVVLGSGNFKYEKVPEWPKMPKYWEFGAASDGAVNSKGEIYIFS
                     RGKHPLTIWTKEGDFISSWGEGTFSANPHGIYIAPNDNVWLVDRDFHIATEYTPSGQT
                     LRTLGEKLAPSPSFQGLPFNMPSGLAISPSGELYVSDGYGGHRVHKFSADGELLLSWG
                     KQGTGPGEFALLHNIWVDERERILICDRENDRIQLFNSGGDFLEEWTDLSSPGDVWIH
                     EDHIYCIEQGPHGGVSIWTLDGEVVSRWKIDEEPGKGSITDGHGITVDLEGSIYVTEI
                     GNGERVSKFVRV"
     gene            2665..3423
                     /gene="larB"
                     /locus_tag="QF838_05080"
     CDS             2665..3423
                     /gene="larB"
                     /locus_tag="QF838_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HJO60090.1"
                     /translation="MEEEKLRGILSEIQSGNTSIEQGVEVFKNLPFENLEFARVDHHR
                     AIRQGFPEVVFGQGKTPEQIQKITKAILDTSGKVVVTRAEPEAYEAVVKSVPNAKYDP
                     MARVITAMLEPNQNLVSGLTVVCAGTSDLPVAMEAAITAETMGCEVNKIFDVGVAGIH
                     RLFDKLEDIQKSKAIVAVAGMEGALPSVIGGLVNCPIIAVPTSIGYGASLGGIAALLA
                     MLNSCSPGITVVNIDNGFGAGYSAANIVLATTLR"
     gene            3441..>4043
                     /locus_tag="QF838_05085"
     CDS             3441..>4043
                     /locus_tag="QF838_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60091.1"
                     /translation="MNIPYNVGIGKSEAPITGITDSRYHSPADIHRVAIITGLSGVRL
                     NESFFSNATRNIDKISTNIGFIASDIKLNSISDPSYVFPPGPESFHELENPEELYIWR
                     WITLDAPDLVIELVETTGRDTFIESIGLPEANKFQFAASSCEADSSLLAALASGLGPT
                     PGAIPGIRITAQNDNVNEILTQIIEKISSTKPTPSEASLQL"
BASE COUNT         1296 a          845 c          868 g         1034 t
ORIGIN      
        1 ctataaaaaa ccaatcgatc ccgcccaatc cctcagatca tcaatcacaa ctcatacaac
       61 atctgaaaaa atttatcata ttaggcgaat cctataacag aagtgctaag gcgactgacc
      121 taatcaaccg tatttctagt aattcgcaag gaaaccttca gaaaacgggt ttcaagctac
      181 ttgaaccaac gggcgttatt aatttagaca taccctatga attagaaaaa ctcggaattc
      241 caattgaatt ttcagaggat ttaatcacca agtcagaaac tttaatacca ttggattacg
      301 attgggaaga tttaacccat ttaccaactt acaccatcga tgatgacgat acgctagatc
      361 gagatgacgc tttctctata gacgaatata accgtcacat atgggtacat ataacagccg
      421 cagccagcat tatagattcc gggtccattt ttgatttgga agcagacaat agaatgtgtt
      481 cggtatattt gccagaaatg acgatcccta tgttaccgac acctatatct gaaaacatat
      541 caacactcct acacgggtcc cgacgattgt gtttatctct cagattagat ttggacgaaa
      601 attcgaattt aatcagacat tcttttgtca aaactgtcat agaaacaaat gagtctctaa
      661 cttataccgt tgcagataat atcttcaatg attccacaga agaattgcat gatgtgttat
      721 caaaattgga agaattcacg actaaaagta ggtcggaaag ggaacgacaa ggtgctcact
      781 cattcaacaa gcctggcatg acgattaaaa tctccaaaga tggcaagata aaggtttcaa
      841 tgtcggcagt tcagtcacgc tccaaagatt tgatagctga actgatgatt gtttataacg
      901 caaaaatatc tgcttttttt atgaccaacc aaatacctgc cttatacaga tcacaggacg
      961 attccacaaa ctctcacgct atggcaggta accagccttt agattggtat ttagccgcaa
     1021 gatctatgaa acccgctaaa gtatcagtaa gccctagtag gcactctggc ttaggtttag
     1081 atttatattg ccaagtaact tcacctctta gacgtttttc agatctatgt cttcaaagac
     1141 aagcaattga atacatcagc agtggtacaa atacgtatgg gccaaaagaa ttatcaacca
     1201 tagggtttaa gtcagaagta cgaattaaag aaatccatcg gcttgaatca aggaggaaaa
     1261 aatattggct cctgaggttt cttgaacaag aaactctaaa tggaaccgtt tccaataggt
     1321 tgtcagcgac agtattggaa aatggcccac tccgaagcgc cctaatagag ctcacagaat
     1381 atccccttag aggaagatgc ttgattcctc aagaatataa cccaggggat gagtgtgagc
     1441 ttaaattaaa cggggttgat ctctgggatt taacagccca atttagcctg ctttgattac
     1501 ctagacaatg aggagatggt cggggtgctc gggatcgaac cgagggcctc ctgttcccaa
     1561 aacaggcgcg ctaccgctgc gccacacccc gaaaaaatag cctaaacaga ttacatccta
     1621 tttctttgaa ggtaaagaaa atatcgaatt tcattttgaa atgcttaatg tccagcatgt
     1681 agaatcagac aattaagatc actaataagc aacaatcaga gacccttaaa cagagaccct
     1741 taaagtcgta aaccggaggt ggccaaatgg ttgtactcgg cagcgggaat tttaaatatg
     1801 aaaaggtgcc ggaatggcca aaaatgccga aatactggga attcggagct gcgtcagatg
     1861 gtgcagtgaa ctctaaaggt gaaatttaca tctttagcag aggaaaacat cctctcacta
     1921 tttggactaa agaaggcgac ttcatatcct cctggggaga agggacattc tctgctaatc
     1981 cacacggaat ttatatagct ccaaacgata atgtttggct cgttgaccgt gattttcaca
     2041 tagcaaccga atatacccct agtgggcaaa ctcttagaac tctgggagaa aaactagctc
     2101 cctcaccctc tttccaaggc ctgcctttta atatgccttc cggcttggct atatctccct
     2161 ccggtgaact gtacgtatca gatggttacg gcggacatcg ggttcacaaa tttagtgccg
     2221 acggcgaact tttattatcc tggggaaaac aaggtactgg ccccggagaa tttgccctac
     2281 tgcacaacat atgggtagac gaaagagaga gaatactaat ttgtgatcgc gaaaatgacc
     2341 gaattcaact atttaacagc ggtggagatt ttctggagga atggacagat ttatccagtc
     2401 ctggggatgt ttggatacat gaagaccaca tatactgcat agaacaaggc ccccacggcg
     2461 gtgtcagtat atggacacta gacggtgaag ttgtctcaag atggaaaatc gacgaggagc
     2521 ctggaaaagg ttcgataacc gacggacacg gaattactgt cgatttggaa ggttcaattt
     2581 atgttaccga aatcggtaat ggagaaagag tatctaaatt cgtaagggtt tgaagcggca
     2641 catttaatta agtggaggca aaacatcgaa gaagaaaaat taagaggaat attgtcggag
     2701 attcaatcag gcaatacatc catcgaacaa ggcgtagaag tttttaagaa tttacctttt
     2761 gagaatcttg agtttgcacg tgtagaccat catagagcca tcagacaggg gttcccagaa
     2821 gtggtttttg gtcaaggtaa aactccagaa cagatacaga aaattaccaa ggctatcttg
     2881 gacacttctg ggaaggtagt tgtcaccagg gctgaaccag aggcctacga ggccgtagta
     2941 aagtcagtgc ctaatgccaa atatgaccca atggctagag tgatcacagc aatgttagaa
     3001 ccaaatcaaa atctggtcag tggtctaacc gtagtttgtg caggaacttc agatctgccc
     3061 gtcgcgatgg aagcggctat cacagctgag actatgggct gcgaggtaaa taaaattttt
     3121 gacgtgggtg ttgctggtat acacagactt ttcgacaaat tggaggatat tcaaaagtct
     3181 aaagcaatag tagctgtcgc cggaatggag ggagcattac cctcagtcat tggaggttta
     3241 gtcaattgtc ccataatcgc tgtgccaact agcattggat atggagctag cttggggggt
     3301 atagccgctc tcctcgctat gttgaatagc tgctcccctg gcattacagt ggtaaacata
     3361 gataacggct ttggagcagg gtattcggct gccaacatag tactagcaac aactctgaga
     3421 taatgaacta tggcgataaa gtgaacatcc catataacgt cggaattgga aaatctgaag
     3481 ctcctatcac aggtattaca gattctcgat atcactcacc cgcagatata caccgagttg
     3541 caataatcac gggcttatca ggcgttcgcc tcaacgaatc gtttttttca aatgcaacca
     3601 gaaatattga caaaatatcg actaacatag ggtttatagc ttcagatatc aaattaaaca
     3661 gcataagtga tccatcatat gtgttccctc ctgggcctga atcattccat gagcttgaaa
     3721 atccagagga gctttacata tggagatgga ttaccttgga tgcccctgac ctcgtaattg
     3781 aattggtgga aacaacagga agggatacct tcattgaatc catcggatta cctgaagcaa
     3841 ataaattcca atttgcagct tcaagctgtg aagcagacag ctctcttcta gccgctctgg
     3901 cctcaggttt aggaccaact ccaggggcaa ttcctggaat aagaattaca gcacaaaacg
     3961 acaatgttaa tgaaattctg acgcaaatca tagaaaaaat ttcaagtaca aagcccaccc
     4021 catcggaggc ttccctgcag ctg
//
LOCUS       DASCNS010000140         4258 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001151488, whole genome shotgun sequence.
ACCESSION   DASCNS010000140 DASCNS010000000
VERSION     DASCNS010000140.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001151488"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="oxc"
                     /locus_tag="QF838_05090"
     CDS             complement(<1..1043)
                     /gene="oxc"
                     /locus_tag="QF838_05090"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HJO60092.1"
                     /translation="MADSVGDVNGEEVGDTENLTDGFNLVVDALKLNDINTIYNVPGI
                     PITDLGRMMQASGMRILSFRHEQNAGYAAAASGFLTKKPGICLTVSAPGFLNGLTALA
                     HATTNCFPMILISGSSEREIVDLQQGDYEEMDQLAVAKPLCKAAYRVLKVEDIGIGIA
                     RAIRAAVSGRPGGVYLDLPGKLFTQVIDIDEGAKSLVKVIDAAPSQLPSTQSVDRALN
                     LLKDAKRPLIILGKGAAYSQADDDIQNLVEKSGIPYLPMSMAKGLLPDTHPQSASAAR
                     SLVLKESDVVLVIGARINWLLSHGKGRTWGDQAKKFIQVDIEPKEMDSNVEIAAPVVG
                     DIGSCVKTLLDGMG"
     gene            complement(1113..1457)
                     /locus_tag="QF838_05095"
     CDS             complement(1113..1457)
                     /locus_tag="QF838_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60093.1"
                     /translation="MTILISFDIDGTMEIGDPAGAVTLEMVKTARAKGIITGSCSDRP
                     MSAQRAIWEQYGVEYDFVCYKHLLSDLKTQFEVEEYYHVGDRDDLDRKYAIQAGFGFF
                     WPDEAAASPLLL"
     gene            complement(1490..2812)
                     /locus_tag="QF838_05100"
     CDS             complement(1490..2812)
                     /locus_tag="QF838_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60094.1"
                     /translation="MATGYAPSSMYRSREGMGIWEHKGKVAAVGVGHSPTERRWDERP
                     ETSVGAWSIMALRNAIADAGVEPDHIDGIVIVPETTTGAFWPDDKPLPQDVINAFVQT
                     DDPLDGVAKLSVEWLLKNMPELTNINYSVYAPVCMSNALNVAAQAVGDGLAHTVLVLK
                     AWHNLEGRYYQGGQNAQDAIGGTSAIRSLWGAPACYGTALQFAEYCRKYGKDHDMMTP
                     FIINSRRNGLMFPEGYWAQHRPEELTPEDYQLARWIAKPANLFDNDIPIMCAASYLFT
                     TAERAKDMKQKPVYVLNHVDSDAVPRGLTPTLDEVEAECALTGRKIYEGAGITAADLS
                     FENMYDGFTQFHQFHLEGLGYRGIGFGDALDFYQTDISIEGPNPVSPSGGNIGSGRSR
                     FWMHTDSIQQLQGRAGGRQITGVNAEIGVSGGPMPLGGNYTVWSATPD"
     gene            complement(2861..3268)
                     /locus_tag="QF838_05105"
     CDS             complement(2861..3268)
                     /locus_tag="QF838_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HJO60095.1"
                     /translation="MKQGVIPDELTAPFWSAANESKLVIQNCSRCDRLQHPPAQDCGQ
                     CKSGENLEWKEMNGTGHIYNYGVVHDCPIRLLQDDQPFNVAVIMLDDDPGIQMYSHLP
                     GTPVDDVPVGAAVEVVFEKSANGQKIPEWKVVN"
     gene            complement(3352..>4258)
                     /locus_tag="QF838_05110"
     CDS             complement(3352..>4258)
                     /locus_tag="QF838_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60096.1"
                     /translation="TSLTAFGQDGPYRDYKSTDLVALAMGGIMHSCGYDDLPGSPPIR
                     PSGDHGNKIASHYGLMGTLAALFNRHFSGKGQYIDASVHEACNSTTEHALPTYLYEGE
                     NVFRQTGRHHSTTPTPKTLCKTADGRYLIVFQLFNNLNSWLSLVGWMAEAGMAEDLQE
                     EKYKDMARGGSRGMRAATDTTDSDHAFEVVRRFIASQTSEDIYRGAQRLKFPWGQVRS
                     PEENLFDPHFAEDRQMFADIPHPEIGEEVSIKYVGRPYRFTESPWDAFRAPLVGEHTK
                     SILSNDIGYTEEDLNALATNGIISY"
BASE COUNT         1115 a         1070 c          833 g         1240 t
ORIGIN      
        1 cccataccgt caagaagcgt tttaacacag gaacctatat cacctactac tggcgcagca
       61 atttcaacat tgctgtccat ttctttgggt tctatatcta cctggataaa tttcttggcc
      121 tgatcacccc atgtcctccc tttgccgtgg gagaggagcc aatttatcct agctcctata
      181 accaatacca catcggactc tttgagtact aaggatcttg cagccgaggc agattgcggg
      241 tgagtatccg gtaaaagtcc tttcgccata ctcataggaa ggtaagggat accacttttt
      301 tctaccagat tctgaatatc atcatctgcc tgcgaataag ctgctccttt ccccaaaata
      361 atgagtggtc gcttggcgtc tttgagtaaa tttaacgctc tgtcaactga ctgagttgag
      421 ggaagctggc ttggtgccgc gtcaattacc tttaccaggg attttgcccc ttcatctata
      481 tcaataactt gagtgaaaag tttgccaggc aaatccaaat agaccccccc ggggcgcccc
      541 gagacagcag cgcgtatcgc tctagcaata ccaattccga tatcttccac tttaagtacc
      601 ctgtaggcag ctttacaaag tggttttgct actgcaagct gatccatttc ctcatagtcc
      661 ccttgttgta ggtcgactat ctctctctca gaagaaccac taatcaaaat cattggaaaa
      721 caatttgtcg tggcatgagc taaagcagtc agaccattga gaaacccagg agctgacact
      781 gttaagcaaa ttccaggctt ctttgtaagg aatcctgatg cagctgccgc atagcctgcg
      841 ttctgctcat gtctaaacga aagtatcctc atcccagaag cctgcatcat acggcctaga
      901 tctgttatgg gaatgcctgg tacgttgtaa atggtattaa tatcattgag tttaagggca
      961 tcaacaacca gattaaagcc gtcagttaga ttttcagtat ctccaacttc ctcaccattt
     1021 acatcgccaa ctgaatcagc catgtggttc tccttgttaa tcgtttcatt ccgtcaataa
     1081 acggcttagt gatggaaaaa tcagggagta ttttaaagaa gcaaggggct tgcggctgct
     1141 tcatcaggcc agaaaaaacc aaaccctgct tgaatcgcat acttccggtc taagtcgtcc
     1201 ctgtccccca catgatagta ctcttcaacc tcaaactgag ttttaagatc ggataaaaga
     1261 tgcttataac aaacaaaatc gtattcgact ccgtattgtt cccaaattgc cctctgggcg
     1321 ctcataggcc tgtcggaaca actccccgtg attattccct tggcccgagc tgttttcacc
     1381 atctccaaag ttactgcccc ggcagggtcc cctatttcca tagtgccgtc tatatcaaag
     1441 ctaattagta tcgtcaaatt attcttccac tttatcaaaa caatatgttt tagtctggtg
     1501 tcgcgctcca aaccgtataa tttcctccaa gaggcattgg gcctcctgaa acaccaatct
     1561 cagcatttac acccgtgatt tgtcttcctc cagcccggcc ctgcaactgc tgtatggaat
     1621 cagtgtgcat ccagaaccta cttctaccgc tgcctatgtt tcctccacta ggggacaccg
     1681 gatttggacc ttcaatactt atatcggttt ggtaaaaatc caatgcatct ccaaatccta
     1741 tccctctata cccaagacct tcgaggtgaa attgatggaa ctgggtaaac ccgtcataca
     1801 tgttttcgaa agataaatca gcggcagtta ttcctgcgcc ttcatatatt ttccttcctg
     1861 taagagcaca ctctgcttct acttcgtcca gtgtgggggt tagtcctcta ggaaccgcat
     1921 cgctgtctac atgattgaga acataaacag gcttttgttt catgtctttg gctctctctg
     1981 ctgttgtaaa cagatatgaa gcagcacaca taattggtat gtcattatca aagaggttag
     2041 ccggtttggc tatccacctt gccagttgat aatcttcagg agtcaattct tcaggtctat
     2101 gctgagccca gtaaccttcc ggaaacatta ggccattccg gcgggaattg atgatgaaag
     2161 gagtcatcat gtcatgatct ttaccgtatt tccgacaata ttcggcaaac tgtaaagctg
     2221 taccatagca agctggcgcg ccccaaagac tgcgaatggc actcgttcca cctatcgcgt
     2281 cctgtgcatt ttgaccacct tggtaataac gaccttcaag gttatgccat gctttcaaga
     2341 caagaactgt gtgagccaaa ccatcaccaa cagcctgggc agcaacgttc aatgcgtttg
     2401 acatacatac cggtgcatag accgagtagt tgatattggt gagttctggc atgtttttca
     2461 gaagccattc aacgcttaat ttggctaccc catctaaagg atcatcagtt tgaacaaacg
     2521 catttataac atcttgtggc aatggtttat cgtccggcca aaaagcccca gtagttgtct
     2581 caggcacaat gacaatgcca tcaatatgat caggttccac tccagcatcc gcaatggcat
     2641 ttcttaacgc cattatgctc caggccccta ctgatgtctc tgggcgttcg tcccacctac
     2701 gctctgtggg tgaatgacct acccctacag cagcgacctt acctttatgt tcccatattc
     2761 ccataccttc ccgacttcta tacattgaag acggagcgta tcctgttgcc atgttaacta
     2821 tctcctttta ctaggctaat aatgcctaca aaaataaaac ctaattaact accttccatt
     2881 ccggtatctt ttgcccatta gcgctttttt caaacacaac ctcaaccgct gcgcctactg
     2941 gaacgtcatc tactggtgta ccaggaagat gggagtacat ttgaatacca ggatcatcat
     3001 caagcataat caccgccaca ttaaacggct ggtcatcttg aagaagtctt attggacaat
     3061 catgcacaac tccgtagttg tatatatgac cggtcccgtt catttccttc cattcaagat
     3121 tctctccaga tttgcattgt ccacagtctt gtgctggagg atgctgcagg cgatcacatc
     3181 gagaacagtt ttgaataacc aatttggatt catttgctgc tgaccaaaat ggcgcagtta
     3241 attcatccgg aattactcct tgtttcactt tagatctcct ttttattttt cctttggcaa
     3301 aacattaata ccgcaggtat atgacttaag cattttgatg attcggtttg attaatagga
     3361 aataatccca ttagtggcta aagcgtttaa atcctcttcg gtgtatccaa tatcgtttga
     3421 tagtatagat ttggtatgtt cgcctacaag aggtgcccta aatgcgtccc acggtgattc
     3481 ggtgaatcga tacggtctgc caacatattt gatactgact tcttctccga tctcaggatg
     3541 aggtatgtct gcaaacattt ggcgatcttc agcaaaatga ggatcgaata aattttcttc
     3601 tggtgatctt acctgacccc atggaaattt caacctttga gcgcctctat aaatgtcttc
     3661 ggatgtttgg gaggcaatga atctcctaac tacctcaaag gcatgatctg agtcagtggt
     3721 atcggtagct gcgcgcatac ccctgcttcc accccttgcc atgtccttat atttttcttc
     3781 ttgcaaatcc tctgccatcc ctgcttcagc catccatccc actaatgaca accatgaatt
     3841 caaattattg aataactgga atacaatcaa atatctaccg tcagccgtct tacataaagt
     3901 cttgggggtg ggcgttgtgg agtggtgtct acctgtttgt ctgaaaacat tttccccttc
     3961 ataaagatag gttggaagag catgctcagt tgtcgaatta cacgcctcat gaactgaagc
     4021 atctatgtat tgacctttac cagaaaaatg tctgttgaat aaggctgcaa gagttcccat
     4081 cagaccataa tgggacgcga tcttatttcc atggtctcca ctgggtctaa ttggtggact
     4141 accaggcaaa tcatcgtagc cacaggaatg cataattcct cccatagcca aagccacgag
     4201 gtcagtcgac ttgtagtcac ggtatggacc atcttggcca aatgctgtca atgaagtc
//
LOCUS       DASCNS010000141         1683 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001153677, whole genome shotgun sequence.
ACCESSION   DASCNS010000141 DASCNS010000000
VERSION     DASCNS010000141.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001153677"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="QF838_05115"
     CDS             <1..541
                     /locus_tag="QF838_05115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HJO60097.1"
                     /translation="PWVLAVPQLILLLFVSFASEADRKPIKAVLHMPYNEGWLRQSMI
                     TVLASIVAIFLYSFTIYKIDIPYLMPPQVPKNILGEEWLMLVNLFAMVLWVPFAEELF
                     FRGFLLTSWIPKYGHSKSIMLVSLVFMFVHSHPGLYFPVFMSSVLISYLFIKTKSLWP
                     AFLSHAVLNLIVVIVAASA"
     gene            594..1385
                     /gene="trpA"
                     /locus_tag="QF838_05120"
     CDS             594..1385
                     /gene="trpA"
                     /locus_tag="QF838_05120"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HJO60098.1"
                     /translation="MENRINLKLISVKSENRPALVPYLTVGYPTIKLSDEIALKALRS
                     GADMLELGIPFSDPIADGPTIQKTGFKALENGVSLSVCMDVLTSIRKENDAAPIIFMG
                     YFNPFLQYGLERFMEEASDRGLDGLIIPDLPIEESVVISEQCRKNGIHLIPLLAPTST
                     EERIKSSCEHAGGFIYCVSLTGVTGAREGLSAGVEELVEKIRNHTDLPVLVGFGVSQK
                     KDVEMISKFAEGAVVGSAFLDHIASSRDGGEISAAGEFIESILVK"
     gene            1401..>1683
                     /gene="aroH"
                     /locus_tag="QF838_05125"
     CDS             1401..>1683
                     /gene="aroH"
                     /locus_tag="QF838_05125"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HJO60099.1"
                     /translation="MSKVQAIRGATTSPSNSSEKILAATTEMLDLIIKENSLKVEDII
                     SAFFTTTQDLNAEFPPVSARKIGWVNVALMCAHEMKVPGALQKCIRVMVH"
BASE COUNT          469 a          279 c          399 g          536 t
ORIGIN      
        1 cccatgggta ttagctgtac cccaattgat tctgttgttg tttgtgagtt ttgcttctga
       61 ggccgatcgt aaaccgataa aagctgtcct tcatatgccg tataacgaag gatggcttcg
      121 acagtcaatg atcaccgtat tggctagtat agtcgcgata tttttatatt cattcactat
      181 ttataaaata gatattcctt atctgatgcc gcctcaagtc cccaaaaata tattgggtga
      241 ggaatggtta atgttggtta atttgttcgc aatggtactt tgggtaccgt ttgcggagga
      301 actttttttt agaggttttc tgttaacctc ttggattcct aaatatggtc attcgaaatc
      361 tataatgctt gtttcattgg tatttatgtt cgtacattcg cacccgggat tgtattttcc
      421 agtatttatg agttccgtat tgatctcata tctattcata aaaactaagt ctttgtggcc
      481 agctttttta tcacatgcgg ttctgaattt gatagttgtg atagttgcgg cttctgcttg
      541 aattgggcag aatgttgtgc acgtaatcct atattgacca ggtggggagc aaagtggaaa
      601 atagaattaa cttaaaactt atctctgtga aatcagagaa taggcctgct ttagtgcctt
      661 atttaactgt tggatatcca actataaagc tgtcagacga aatagccttg aaggctctta
      721 gatccggggc tgatatgttg gaattaggga taccttttag tgaccctata gctgacgggc
      781 caactattca aaaaactggt ttcaaggccc ttgaaaacgg tgttagcctt tcagtgtgta
      841 tggatgtttt aactagtatc cggaaagaga acgatgctgc tccaattata ttcatgggtt
      901 atttcaatcc gtttcttcaa tatggactgg aacggtttat ggaagaggca tcagatcgtg
      961 gcctcgatgg tttgataatt cctgatctac cgatagaaga gtccgtggtt atatctgagc
     1021 aatgtaggaa aaatggaata caccttattc ctttattggc tcctaccagt actgaggaaa
     1081 gaataaaatc ttcctgtgaa catgcaggtg gatttatata ttgtgtcagc cttaccggtg
     1141 taactggagc gcgtgaaggt ttatctgcgg gtgtagagga gttggtcgaa aaaatcagga
     1201 atcatacgga tttgcctgta ttggttgggt ttggagtttc tcagaaaaaa gatgttgaga
     1261 tgatatctaa atttgcagaa ggggcggtgg ttggcagtgc atttttggat catatagcga
     1321 gttccagaga cgggggcgag atatcggcag ctggagaatt catcgagagc atattggtga
     1381 aataaaacag agaggttgta atgtctaaag tacaagcaat aagaggtgcg acaacatccc
     1441 caagtaatag ttcggagaaa attctggctg ctactactga gatgttggat ttaataataa
     1501 aagaaaactc gttgaaagtt gaagacatca ttagcgcatt ttttacaacg actcaagatt
     1561 taaatgctga atttcctccg gtgtctgcta ggaaaattgg atgggtaaat gttgccctaa
     1621 tgtgtgctca cgaaatgaaa gttcctggag cactgcaaaa atgcatccga gtcatggtgc
     1681 acg
//
LOCUS       DASCNS010000142         4596 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001154791, whole genome shotgun sequence.
ACCESSION   DASCNS010000142 DASCNS010000000
VERSION     DASCNS010000142.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001154791"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2357
                     /locus_tag="QF838_05130"
     CDS             <1..2357
                     /locus_tag="QF838_05130"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HJO60100.1"
                     /translation="CTVVVSELSEGEHYIYATAISEMGTSSSRSDSVLITIDTEIETP
                     SDPDLTIGSDTGKETDDDLTSVDSPTFSGIGEPYARIEIINSISSVLGTDMVASTGLW
                     KVTADEQSLEDGTHLIFARAIDEAGNQSDLSDSIEITVDTEAPERMSEPKPNQNGEIS
                     GWLGPDFLITLYNDGKEVSTVQTQDGGDWVFSMNELPYGENALTVTATDAAGNESEMS
                     VQNLITMNRETPQGQGQGQGANEAPIIDAMFGNNPNNNMWEETRDTDDGFPEFRGWGE
                     PFMDIVLRGNGDRLGESIVQADGSWIIYPDREMREGTYLIIAEQVDPSTGKIGPPSRD
                     FNIDIHPAGSGNVMIPTTPQMYWNAGGRQMSGTEADASHDVWFEGYADPNVLIVLLKS
                     SEQMGDTYSDQNGHWVVYPYEVLTPGNYDITAYAENDFSMQSGWSQSAYINIGSAPAM
                     KPPVIEGMLFFDSYGNTILTDSTVTGYVGFRGYASGSDVEVHIYSDGEELAHAQVDYD
                     GRWEAWSNFMLKPGDYTVTAKARDWNSGMEVESEPYYAEITDVTPPTIYGVRDQWGDW
                     TTVHDASLVGPMMLGGSAGPNMRIEVFVDGQWIADSGTDANMDTWEIGPTPEACFPQG
                     TYKVTAYAYANGTMQSEESDPVWIDVIGDGRDMCDMMRNMMDSGSSMMQGGSMGENDM
                     NMFGNDMFGNNSGNMQGGNDMFGNNSGNMQGGNDMFGNNSGNMMGGGDDRNNFAGFGS
                     DSGSGFNNGGSGGFNSGGSGGFNSGGSGGFNSGGSGGFNSGGNN"
     gene            2413..3258
                     /locus_tag="QF838_05135"
     CDS             2413..3258
                     /locus_tag="QF838_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HJO60101.1"
                     /translation="MAYLIEISSGPQQGERQILTDKHVIVGRSPSCDVTIDDPYVSGN
                     HIEFWSAGDMAFARDLNSSNGSSHNGNSINPADTLTLNSGDRIDLAEGKAIIVVLQEA
                     ADAKNISAPPSGTITFLFSDMYGSTDLVNELGDIKFREISRTHEGVLGDAIREHEGYI
                     VKEMGDGFMAAFASSRNAVICAMEIQKSLKTLRAKQPELPINVRIGLNTGEAILENGD
                     YFGRAVSEAARISSKAEAEQILISSVTKRMADSAGDLKFGEEMEFELKGLPGDHTVFE
                     VFWEE"
     gene            complement(3275..>4596)
                     /locus_tag="QF838_05140"
     CDS             complement(3275..>4596)
                     /locus_tag="QF838_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HJO60102.1"
                     /translation="VKLHDDGTHGDDIANDGLFSRACISKTDLEITLTDFDNLAASYR
                     FRDGMLRIVDASLRGTVPVTTLSPDLMATENAMFMTLGAESYKKVRSGNTFDLQNPKT
                     CEACEEVLNVFGDVLDHIILVPDEPMVDKNTNEYASYLRTSDSTKGIMTYGDPICDPT
                     GWNNHGKSGGINKDYRDLKFGCPSKFLNGGDYPRLKGIIWAGGMEGLNAAFGRWVGIG
                     PRNGNFPLNSGVSWNSTDRKNIDANTTVKGPYPITESLWDPERGKPHQVQVLSNNKWQ
                     DARIEAIGGGVFSLTNLDTDHYQFDDIFLYMMGFISYENALSEKYYYLQELDLSNCTT
                     GAQNLRCSDQDTVIEETQYKNAIEFTVQNMLSTYGPRDPVYSEETTHLNAPVIVLSES
                     VPSDASIVWHDLLFDWWGTKEGYEVNYGGHTWKSVTKGTATISTGYK"
BASE COUNT         1278 a          916 c         1162 g         1240 t
ORIGIN      
        1 cctgtacggt cgtggtcagt gaactttcag aaggagaaca ttacatatat gcaacagcta
       61 ttagtgagat ggggacttcg agttcaagat cagattctgt tcttataacg attgatacag
      121 aaatagaaac gccgtcagac cccgacctta ctatagggtc agatactggt aaagaaactg
      181 atgatgattt gacttcagta gactctccca cattctccgg catcggcgaa ccctatgcta
      241 ggatcgaaat aattaatagt atttcatcgg tacttggcac cgatatggtt gcttcgacag
      301 gattgtggaa ggtcactgca gacgaacagt ctctggagga tgggacacac cttatctttg
      361 cgagagccat cgatgaggca ggaaatcaga gtgatctctc ggactctata gaaatcacag
      421 ttgatactga agcgcctgag cgaatgagtg aaccaaaacc caatcaaaat ggcgagataa
      481 gcggatggct gggaccagat ttcctaatca ctctctataa cgatggaaag gaggtatcca
      541 cagtccaaac gcaggatggc ggagactggg ttttctcaat gaacgaactt ccatatggtg
      601 aaaatgcact tactgttact gctacagatg ctgctggaaa tgaaagcgag atgtccgtac
      661 aaaatctaat aaccatgaat cgggaaaccc cccagggcca gggccaaggc caaggggcca
      721 atgaagctcc gataatagac gctatgttcg gcaataatcc caataataat atgtgggaag
      781 agaccagaga tacagacgac ggtttcccgg aattcagagg atggggcgag ccatttatgg
      841 acatagtact cagagggaac ggggataggc taggagaaag tatcgttcaa gcagatgggt
      901 catggataat ctatcctgat agagaaatga gggaaggcac ctacctcata atagctgagc
      961 aggtggatcc atctacaggc aaaataggac ctccctctag agactttaat atagacatac
     1021 atcctgcggg gtctggaaac gttatgatac cgaccacacc acagatgtat tggaatgctg
     1081 gtggtaggca gatgagcgga accgaggctg atgcatccca cgatgtgtgg tttgaagggt
     1141 acgctgatcc taacgtgtta attgttttgc tgaaaagcag tgagcagatg ggtgatacat
     1201 attccgacca gaacggtcac tgggtcgttt acccatatga agttttaaca cccggcaatt
     1261 atgatattac tgcctatgct gagaatgact ttagtatgca aagtggctgg tctcaaagtg
     1321 catatataaa tataggttct gcacctgcta tgaaacctcc ggtgattgaa ggaatgctct
     1381 tttttgactc atacggcaat acaatattga ccgatagtac tgttacgggt tatgtcggat
     1441 tcaggggata tgccagtggc tctgatgtgg aggtccacat ctactctgat ggggaagaac
     1501 tcgcccatgc ccaggttgac tatgatggcc ggtgggaagc ctggtcaaat tttatgctca
     1561 agcctggtga ttataccgtg accgcgaagg caagggattg gaattctggt atggaagttg
     1621 aatccgagcc atattatgca gaaatcacag atgtaacacc tcccacaatc tatggagttc
     1681 gagaccaatg gggtgattgg acaactgttc atgatgcaag cctcgtagga ccgatgatgc
     1741 ttggagggag cgcagggcct aatatgagaa ttgaagtttt tgttgatggc caatggatag
     1801 ctgacagtgg tacagatgcc aacatggaca catgggaaat aggaccgact cctgaagctt
     1861 gcttccctca aggtacatac aaagtcacgg cttatgctta tgccaacggc accatgcaga
     1921 gtgaggaatc tgatcccgtt tggatcgatg tgattggaga cgggagagat atgtgtgaca
     1981 tgatgagaaa tatgatggac tcaggatctt caatgatgca aggcggatcg atgggtgaaa
     2041 atgacatgaa catgtttgga aatgacatgt ttggaaacaa cagcggtaac atgcagggtg
     2101 gaaatgacat gtttggaaac aacagcggta acatgcaggg tggaaatgat atgtttggaa
     2161 acaacagtgg caatatgatg ggcggtggtg atgatagaaa taatttcgca ggatttggaa
     2221 gtgacagcgg cagtggtttt aacaacggcg gcagcggcgg gtttaacagc ggcggcagcg
     2281 gcgggtttaa cagcggcggc agcggcgggt ttaacagcgg cggcagcggt gggtttaaca
     2341 gcggcgggaa taactaactt tctgtgagcc ccatttacat ttatttccaa aagctggtaa
     2401 attattgggg taatggctta tttaattgaa atatcatctg gtccccaaca aggggagcgc
     2461 caaattctga ctgacaaaca tgtaattgtt ggcaggtctc catcctgcga tgtgacgata
     2521 gatgatccat atgtctcagg caaccatatt gaattttggt ctgcaggtga tatggccttt
     2581 gcaagagatt taaacagttc taatggatcc tctcacaacg gtaattctat aaaccctgcc
     2641 gatactctga ctctcaacag tggggatcgt atcgatttgg ccgaagggaa agctataatc
     2701 gtcgttttgc aggaagcagc ggatgccaaa aacatttcag ctccgcccag tggtaccatc
     2761 acatttttat tcagtgatat gtatggttcg actgatttag ttaacgagct cggggatata
     2821 aaattcagag aaatatctcg aacgcatgaa ggtgtccttg gtgatgcaat aagagagcat
     2881 gaaggttata tcgttaagga gatgggagat gggttcatgg cggcttttgc aagtagtaga
     2941 aatgccgtga tatgtgctat ggagattcaa aaatccttga agactctccg ggcaaaacaa
     3001 cccgagttac ccataaatgt aaggattgga ttgaacacag gggaagcgat attggagaat
     3061 ggggattatt ttggcagggc tgttagtgag gccgccagaa tatcttccaa agcagaagct
     3121 gagcaaattt tgatttcttc ggtcactaag agaatggctg attctgctgg tgatttgaaa
     3181 ttcggagagg aaatggaatt cgaattgaaa ggcctgcctg gggaccatac tgtttttgag
     3241 gtcttttggg aagaataggc agctttagat caacctattt atatccagtt gaaattgtgg
     3301 ccgtgccttt tgttaccgac ttccatgtat gcccaccata gttgacttcg tagccttctt
     3361 ttgttcccca ccagtcaaat agtaggtcgt gccatactat tgatgcatcg gatgggacac
     3421 tttctgaaag tacaataacc ggcgcgttca gatgagtggt ttcttcagaa tacacaggat
     3481 ccctggggcc gtaggtgctg agcatgtttt gcaccgtgaa ttcaattgcg tttttgtatt
     3541 gtgtttcctc tatgacagtg tcctggtcgg aacaccgtag attttgagct ccggtggtac
     3601 agttactcaa gtctaattct tgaaggtagt agtatttttc tgaaagcgcg ttttcgtagg
     3661 atataaaacc catcatatat agaaaaatgt cgtcaaattg gtagtgatca gtatcaagat
     3721 tagtcaacga aaacactcca cctccaatgg cttctatcct cgcatcttgc catttgttgt
     3781 ttgatagaac ctgtacttgg tgaggtttgc ccctttcagg gtcccataac gattccgtta
     3841 tgggataagg tcctttaacg gtagtgttag catcaatatt ttttcgatca gttgaattcc
     3901 agcttacgcc actgtttagg gggaagttac cattccgtgg tcctatgcct acccaacggc
     3961 caaatgccgc gtttagaccc tccattcccc ctgcccatat gattcctttt aacctgggat
     4021 aatcccctcc gttcaggaat ttcgaagggc aaccaaattt taaatctcga taatccttat
     4081 ttataccgcc ggatttaccg tggttgttcc atccggttgg atcacagatt ggatcaccat
     4141 atgtcatgat tcctttggtg ctatctgagg ttcgtaaata acttgcatat tcatttgtat
     4201 tcttgtcaac catcggttca tctggaacca gaataatatg gtctaataca tcaccgaaga
     4261 catttaatac ttcttcacag gcctcgcatg tttttgggtt ttggagatcg aacgtgttgc
     4321 ctgaccgcac tttcttatag gactctgcac caagggtcat gaacattgca ttttctgttg
     4381 ccatgaggtc tggtgataag gttgttactg gaaccgttcc ccttagactg gcgtcaacga
     4441 ttcttaacat accatcacgg aatcgatatg atgcggctaa attatcgaaa tcagttaagg
     4501 tgatttctag gtcagtcttg gaaatgcagg ctcgggaaaa taggccatcg ttggcaatat
     4561 catctccatg agtaccatcg tcatgcagtt ttacag
//
LOCUS       DASCNS010000143         1544 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001167938, whole genome shotgun sequence.
ACCESSION   DASCNS010000143 DASCNS010000000
VERSION     DASCNS010000143.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001167938"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /gene="sufC"
                     /locus_tag="QF838_05145"
     CDS             <1..423
                     /gene="sufC"
                     /locus_tag="QF838_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743652.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HJO60103.1"
                     /translation="LKIRDFDKLLEEKRQLVGMDPDLVRRSVNEGFSGGEKKRNEILQ
                     LALLDPSLALLDETDSGLDIDALRIVSHGVNIFKSPEKSVLIVTHYQRILDYITPDYV
                     HVLVDGKIVRSGDSRLAMELEEKGYDWVKDGAGEEVNP"
     gene            423..>1544
                     /locus_tag="QF838_05150"
     CDS             423..>1544
                     /locus_tag="QF838_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HJO60104.1"
                     /translation="MQEASLIANKYEEDFDNLSSIGPEWSSDLRNKAMDTFREIGFPI
                     QRKGNEKWKYTNARPIVDVEYSSAKFEKIDLDYIKSIAPWHESWINILLVNGYYQETF
                     SDNLPTNIQISNMASSDLDTNIFRTVGGIMDYSDDGFAALNTAFMSDGAIITVPEGEN
                     SGIAINLIFFNQGLSDTVNHPRVLITSGANSTVSVIENYVGGSRDRTFTNSVSEIYVG
                     ENATVNHYRLMKETDDTYDVGYGRVRIDTDGTFNSRSFFRGCGIGRYDLNVSIEGENA
                     SCDLQGLYFTNDAQHMDNFINIDHVRPNCQSNLFYKGILAGKSRAVFGGTVLVRKEAQ
                     KTESVQSDKNLILSPYAEVDSKPALFIYADDVKCAHGATA"
BASE COUNT          472 a          274 c          365 g          433 t
ORIGIN      
        1 ctcaaaataa gggacttcga taaattgctc gaagaaaagc gccagcttgt gggaatggac
       61 ccagaccttg tgcgccgttc tgtgaacgag ggattttctg ggggggaaaa aaaacggaac
      121 gaaatattac aactagcact attagacccc tccttagctt tattggatga aacagactct
      181 gggttggata tcgacgcttt gagaattgtt tcccatggag taaatatttt taaaagtcct
      241 gaaaagtcag ttttaatcgt cacccactac cagcgaattt tggattacat tactcctgat
      301 tatgtgcacg tattggtaga cggtaagata gtccgctctg gtgacagtag acttgctatg
      361 gaattagaag aaaagggata cgattgggta aaggatggtg ctggtgaaga ggtgaaccct
      421 taatgcaaga agctagctta attgcgaata aatatgaaga ggattttgat aatctttcct
      481 ccatcggccc tgaatggtca tccgatttga ggaataaggc aatggatacg tttcgggaaa
      541 taggttttcc gatacaaaga aaaggcaatg aaaagtggaa atacactaac gccagaccga
      601 tagtagatgt cgaatattct tcggcaaagt ttgaaaaaat agatttagat tatattaaat
      661 cgatagctcc atggcacgaa tcttggatta atattctgct tgttaatgga tattatcaag
      721 agactttttc agataatttg cccacaaata tacaaatttc caatatggcc tcaagtgacc
      781 tggatacaaa tattttccgt actgttggcg gtattatgga ttattcagac gatgggtttg
      841 ccgcattaaa cacggcattc atgtcagatg gagcaataat aaccgttccg gagggagaga
      901 actccggaat tgcgattaac ctcatttttt tcaatcaagg tttgtctgac acagtgaatc
      961 atcctcgagt tttgataact tcgggtgcaa actccacggt aagtgtcatt gagaattacg
     1021 ttggaggtag tagggatcgg acatttacta attccgtttc tgaaatatat gtaggtgaga
     1081 acgcaacggt taaccattat cgtttaatga aggagactga tgacacctac gacgttggct
     1141 acggcagggt aagaatagat actgatggaa cctttaattc tagatcgttt ttccgtggct
     1201 gtggcatcgg acgttatgac ttaaatgtgt ctattgaagg tgaaaatgcc tcctgtgatt
     1261 tgcaaggttt gtatttcaca aatgatgccc agcatatgga caatttcatt aatattgacc
     1321 atgtacgtcc gaactgccag agcaacctat tttacaaagg aatactggca ggcaaatcaa
     1381 gggcggtgtt tggaggcacg gtcttggtta ggaaagaagc acagaagacg gagtccgtac
     1441 aatcagataa aaatctaata ctgtcaccat atgccgaggt tgatagtaag ccggcattgt
     1501 ttatatacgc cgatgacgtc aaatgtgccc acggggctac ggcc
//
LOCUS       DASCNS010000144         3867 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001170508, whole genome shotgun sequence.
ACCESSION   DASCNS010000144 DASCNS010000000
VERSION     DASCNS010000144.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001170508"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="QF838_05155"
     rRNA            complement(<1..1436)
                     /locus_tag="QF838_05155"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1786..2301
                     /locus_tag="QF838_05160"
     CDS             1786..2301
                     /locus_tag="QF838_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60105.1"
                     /translation="MKTKSQNITINLLDSEETIHEGDTFILTNQRLMVSSKPGRTSNV
                     WEQAELEECLDPKLKNAGKTTRKWLGYRLLLIGFAMIGLQMIPYLFFGANILGKLPGL
                     IESLYFLASMLTATAGIYLTLGSYLNRPPHTSVLFGVPGAKDLLAIFPGWDSEEASEL
                     VSKYRRAKRNV"
     gene            2355..3521
                     /locus_tag="QF838_05165"
     CDS             2355..3521
                     /locus_tag="QF838_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60106.1"
                     /translation="MIDSIGTLYAGHIDLESVGFDSIPANDRTYSAEELNTVYAKAEA
                     IAKVMDSNGYDILWMAEHHFQKEGYECIPNVLMLAVHLAHLTKNIRIGCGFNVSPMWH
                     PLRMAEDYATAEILTNKRVVFGVGRGYHTREVESFGNPMLDGDANRELFEEQVDVMFK
                     AFHSDSFSHVGENYVIPPEIPYRGYDLKEITLVPRPLQRPVECWQPIVSASERGLSFM
                     AKHAIKGVIGGGAAPGGASENVIKEWQRVQSQYGIETDLGGNLCIGFSFHIADSEKQA
                     IEEARPFFEENMKMFAPLGMVKGLTDDQIGQLSKGSQFAKTANLPTLEQAVESGSWLV
                     GTPESIAEKLLEIQARYPGLKAINVGQVIGTPEKVILEQLDRFGKEIMPKVKTP"
     gene            3568..>3867
                     /locus_tag="QF838_05170"
     CDS             3568..>3867
                     /locus_tag="QF838_05170"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HJO60107.1"
                     /translation="MKNMKEKRVLVITPHPDDAESGAGGTIAKWSHLGANIVLVVCTN
                     GNKGTSDRSISSEELAQTRRKEQLESAKLLGLTNVVFLDHPDQGVQNGDPFRKELV"
BASE COUNT         1054 a          884 c          856 g         1073 t
ORIGIN      
        1 ttcgctatac gctacggctt aggcccgact taccctacgc tgatcagcat tgcgtaggaa
       61 accttatgct ttcggtgtcc tggattctca ccaggattgt cgctactcat gccggcattc
      121 tcacttctca gcgctccact ggacctcacg atccagcttc gacgccctga caacgctccc
      181 ctaccgctcg ctataagcga gcccgcggct tcggtggtgt gctttagccc cgttacattt
      241 tccgcgcaaa attcctcgac tagtgagcta ttacgcactc tttaaaggat ggctgcttct
      301 aagccaacct cctagctgtt ttagcaattt aacttcgttt cacacttagc acacacttag
      361 ggaccttagc ctgcgatctg ggctgtttcc ctctcgacaa tggagcttat cccccactgt
      421 ctcactgccc gggaccatct tgcggcattc gtggtttggt taagtttggt aggctcacgc
      481 ccccttacct atccagtgcc ctacctccac aagactaccc gaacgctgta cctaaataca
      541 tttcggggag aaccagctat ctccgtgttc gattggcatt ttacccctac cctcagctca
      601 tcccagcctt ttgtaccaga cacgggttcg gtccttcacc cagagactat ctgggcttca
      661 acctggccaa gggtagatca cacggtttcg ggtctaatcc atacaactca acgccctgtt
      721 cggactcgct ttcgctatgg ctccggaatt ccaattcctt aaccgtgctg cataaattaa
      781 ctcgccggct cattcttcaa taggcacgca gttagcccga cgcatcgagc cttctacggc
      841 ttgtaagatt acggtttcag gtctatttca ctcccctctc ggggtacttt tcacctttcc
      901 ttcacagtac tggttcacta tcggtgtcca agagtattta gccttggaga gtggtctccc
      961 cagattccca caagatttct cgtgtctcgt ggtacttaac aaattctcaa gagtttcgtc
     1021 actttcgcct acaggactat caccttctat ggtcgtcctt tccaggtgcg tttggctagt
     1081 tcagaaattt cttactcttc gccctcattc gaggttcagg cggagaagtg ttgcaacacc
     1141 aaacggacat aggacctcat tccactaagc ccgtctggtt taggctattc ccctttcgtt
     1201 cgccactact aggggaatct cggttgattt atactcctcg cgttaattag atgtttcagt
     1261 tcacgcggtt ccctttcctt acggaatgtc ctggtacaac ccaggacggg tttccccatt
     1321 cgggtatccc cggttaagct cgttagacag ctagccgagg cttttcgcag tcttaccacg
     1381 cccttcttcg gctcttggac ccaaggcatc caccgtaatc ccttaataac ttgacccaca
     1441 ttaaggcaat tctattgttc ttgtttgcct gttcctggct tttggtgcat aaataaacca
     1501 aaagccgtga cctcataagt gaggtcggga ctcgttataa ttcgacagaa ttcagttgtt
     1561 aaggtgcaaa aaaaaagccg ggggagatcc cccgaccgaa gtcaggattc tataggtctt
     1621 tatgtttaaa tctctgtagt catcctattt caaactctga ggataaagtc tatacctgac
     1681 taacttgcta gtcaacatgg aatattgcct aattttcaac tcaaatcaaa taggcattta
     1741 ttttacctat taacattcat accctgcagt ataatttgct tcgtaatgaa aacgaaaagc
     1801 caaaatatca caataaatct ccttgattcc gaagagacaa ttcatgaagg agacacgttt
     1861 atattaacca accaacggtt gatggtttca agcaaaccag gcagaacttc caatgtgtgg
     1921 gagcaagctg agttggaaga atgcttggac ccgaaattga aaaatgcagg aaagacaact
     1981 aggaaatggt tgggatacag attgctcctt attgggtttg ccatgattgg cctgcaaatg
     2041 atcccctatc ttttttttgg tgccaatatt ttgggtaaat tacctggttt aatagagtca
     2101 ctttattttt tagcctctat gcttacagca acagcaggga tatatttaac tttagggagc
     2161 tatttgaaca ggccgccaca tacgtcagtt ttattcggtg ttccgggtgc caaagacctt
     2221 ttggctattt tccctggctg ggatagtgaa gaggctagcg aacttgtatc taaatatagg
     2281 agagcaaaga ggaatgtgta ggctgatttt tcaataagaa aatacgttta ggtacaaaat
     2341 ttaggaggtc aagcatgata gatagcatcg gaacattgta cgcaggacat atagaccttg
     2401 aatcagttgg atttgattcg attcctgcta atgaccgcac ctattcagca gaagagctga
     2461 acaccgttta tgcgaaagct gaagcaatag ccaaagttat ggactccaat ggctatgata
     2521 ttttgtggat ggcagaacac cattttcaaa aagaaggata cgaatgtatc cctaacgtgt
     2581 taatgctagc tgttcatctg gctcatttga ctaaaaatat aaggatcgga tgtgggttca
     2641 atgtgtcacc gatgtggcat ccattgagaa tggcagaaga ttatgccacg gctgaaatat
     2701 taaccaataa aagagtagtt tttggagtgg gtaggggtta tcacacgagg gaagttgaat
     2761 ctttcggcaa cccaatgttg gatggagatg ccaacaggga attatttgag gaacaggttg
     2821 atgtaatgtt taaggcattt cattcagatt ccttttctca tgtgggggaa aattacgtga
     2881 ttccgcccga gattccctac cggggatatg acctaaagga aataaccttg gtgcctagac
     2941 ctctacaacg cccggtagag tgctggcaac ccatagtcag tgctagtgaa aggggtttgt
     3001 cttttatggc caagcatgcc atcaaaggag ttattggagg aggtgcggct cctggtgggg
     3061 ccagcgaaaa tgtgatcaaa gagtggcaaa gagtacagtc acaatatggg atagaaacag
     3121 acctaggcgg aaatctgtgc ataggctttt cattccacat agcagatagt gaaaaacaag
     3181 caattgagga agccagacct tttttcgaag aaaacatgaa aatgttcgct cctttaggaa
     3241 tggtaaaagg ccttactgat gatcaaatag gccaactatc caaaggatca cagtttgcca
     3301 aaaccgcaaa tttgcctaca ctcgaacaag cagtagaatc agggagctgg ctggtgggca
     3361 ccccggaatc catcgccgaa aaattattgg aaattcaagc tagatatcca ggtctcaaag
     3421 ctataaacgt tggacaagtt ataggaactc cagaaaaagt gatacttgag caattggatc
     3481 gatttggtaa ggaaatcatg ccgaaggtaa aaaccccata attttcaaag gcttgacact
     3541 ttaattaaga ggattaaaac caaattcatg aaaaatatga aagagaaaag agttttagtt
     3601 attactcccc atccagatga tgctgagagt ggagcagggg gaactatagc gaaatggtct
     3661 caccttggtg cgaacattgt tttggtggtt tgtactaatg ggaataaagg cactagtgat
     3721 cgctccatat cctccgaaga attggcacaa actcgaagaa aggaacagtt agaatctgcc
     3781 aaattgttag gccttacaaa cgttgttttt ctagaccatc ctgatcaagg tgtacagaat
     3841 ggagatccct ttaggaagga gctagtg
//
LOCUS       DASCNS010000145         1845 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001172093, whole genome shotgun sequence.
ACCESSION   DASCNS010000145 DASCNS010000000
VERSION     DASCNS010000145.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001172093"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="QF838_05175"
     CDS             <1..631
                     /locus_tag="QF838_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60108.1"
                     /translation="GVLHPDKKEFQEMVRESHFHGFPVAIHSVDMTSIDMILEVLKND
                     YLLGDRIEHASELRDDQILKMASIGVSVSTQPSFIYERGDNYLSTLPASDINSLYRIN
                     SLRKAGITVSASSDSPVTYPDPMKTIYSAMTRKTKSGDVLNPKEAISALDSLKMLTTD
                     SVTISGLGNGSFQDPKTLNEDSLVLETNLASGETGNILGTKISRIKDFK"
     gene            684..>1845
                     /locus_tag="QF838_05180"
     CDS             684..>1845
                     /locus_tag="QF838_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60109.1"
                     /translation="MTSTIADILISGGLLVSGSSIKKMDVSVLGEKIVEVAPYPSGIK
                     AKRVIDGSGLYVLPGLIDAHNHPINLDKMDSFTLSAAFGGITTVIPFMQNMRSRGIEG
                     TTSETIGRFIEESNEISYLDFGVHAILFGDDDVEVEVPKLIDNGVISFKMYMTYPRRG
                     MMMPDDRMLRAMSLASDAGGIAMVHAENGYCIDFLTDNFIEDGLTSREYYAKSQPRIL
                     EVEAANRAATYASVTNCPLYVVHLSAREVLEVLNRYREEGKHVFTETCPQYLDLTNET
                     LMEHGALAKIGPPLRDREDNEAMWEGLQNNVIDTIASDFCGFSKAHKYSGGRSSAAVE
                     AVDEIDEDGSIFEASFGGNWTEQMLPVVYEEGVNQGRITMMRLVQVMCEINLK"
BASE COUNT          561 a          345 c          437 g          502 t
ORIGIN      
        1 tggtgtcctt catccagata aaaaggaatt tcaggagatg gttagagaaa gtcatttcca
       61 cggctttcct gtagctattc attctgttga tatgacttca atcgacatga tccttgaggt
      121 tttaaagaat gattatctac ttggtgaccg tatagagcat gcctcagaac tgcgggacga
      181 ccagatactt aaaatggcaa gtatcggagt ctcagtttcc acccaaccat cattcatata
      241 tgaaagagga gacaattatt tatccaccct acctgctagt gatataaatt cactctatcg
      301 aatcaatagc ttgaggaaag ctggtatcac tgtttcggcg tcctcagact ctcctgttac
      361 ctaccctgac ccgatgaaaa ctatttattc ggctatgaca cgcaagacaa aatctgggga
      421 tgttctcaat ccaaaagagg ccatttcagc acttgatagc ttaaagatgc tcactaccga
      481 tagtgttact atttctggat taggtaatgg atcgtttcaa gaccccaaaa ctcttaatga
      541 agattccttg gttttagaga caaatttagc ttcaggcgag accggtaata tactggggac
      601 taaaatttcc cggatcaaag atttcaaata actaacagta tagaaagaaa gacaaatcag
      661 aatcattgaa aggagctttc tagatgacat ccacaatcgc cgacattctg atttcaggtg
      721 gcctcctagt cagcgggtca tctataaaga aaatggacgt gtctgtctta ggagaaaaaa
      781 tagtggaggt ggctccatat ccttctggta ttaaagcaaa gcgggtgatc gatgggtcag
      841 ggctttacgt gttgccaggt ttgattgatg cccataatca cccgattaat ttagacaaaa
      901 tggacagttt taccctcagt gccgccttcg gagggattac gactgtcatt ccgtttatgc
      961 aaaatatgag aagcaggggg atagaaggta caacctcaga gacgataggg agattcatcg
     1021 aagaatctaa tgaaatttca tatctagatt ttggagtgca tgccatttta tttggcgatg
     1081 acgatgtcga ggtggaagtc cctaaattga tagataatgg ggtcatttca ttcaaaatgt
     1141 acatgaccta tcctagaaga ggcatgatga tgccggatga tcgaatgctt agggcaatgt
     1201 ccttggcgtc cgatgccgga gggattgcga tggtccatgc ggaaaacggg tattgtattg
     1261 atttcctaac agataatttt atcgaagatg ggcttacttc tcgtgaatat tacgcaaaat
     1321 cgcaacccag gatcctggaa gttgaggctg ctaaccgggc cgcaacatat gcctctgtta
     1381 ctaattgccc actttatgtg gtccatttga gtgctagaga agtcctagaa gtactgaata
     1441 ggtatcgaga ggaaggcaaa catgtattta ccgaaacctg cccccaatat cttgatttaa
     1501 caaacgagac attaatggaa cacggagcgc tagcaaagat aggaccacct ttgagagaca
     1561 gggaggacaa tgaagctatg tgggaagggt tacaaaacaa tgtaatagat actatcgcaa
     1621 gtgatttttg tggttttagc aaagctcata aatattccgg tggtagaagt tcagccgcgg
     1681 tggaagcagt agatgaaatt gatgaggatg gcagtatttt tgaagctagt ttcggaggca
     1741 actggacgga gcaaatgttg ccagttgttt atgaagaagg ggtcaatcaa ggacggatta
     1801 ccatgatgag actcgttcag gtgatgtgtg agatcaactt gaaaa
//
LOCUS       DASCNS010000146        14411 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001174841, whole genome shotgun sequence.
ACCESSION   DASCNS010000146 DASCNS010000000
VERSION     DASCNS010000146.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 14411)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14411)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14411
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001174841"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(2..865)
                     /locus_tag="QF838_05185"
     CDS             complement(2..865)
                     /locus_tag="QF838_05185"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HJO60110.1"
                     /translation="MISSYIALTKPKIIVLLLITTVGGLFLASDGFPDLTIAAAVLIG
                     GTLAAGGANSLNHFWDRDIDIKMKRTTTRPVASGAVPPTNALIFGILLNLVAFAVLYR
                     YTNLLSALLTLSATLFYIFVYTMVLKRTTPQNIVIGGTAGSIPPTVGWVAVTNSIMDP
                     APIFLFLIVFFWTPPHFWALSLILKDDYENAGIPMLPVVSGIAATKTQIFIYTWILLV
                     LLLAATYFVEELSIIFGISSVLLCAGFIWSTWLLKRRDNIELAMPIYLYSMAFLALLF
                     VVMVAESFILI"
     gene            complement(869..1804)
                     /locus_tag="QF838_05190"
     CDS             complement(869..1804)
                     /locus_tag="QF838_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HJO60111.1"
                     /translation="MNRTTTPRPNPRNTLKWLSIITLISAFSQVTLGGYVRSSESGLG
                     CPDWPLCHGQIIPPFEFHTLVEYSHRLNASLLMVLVGTLFLLCWLRFREEKIVTFLAA
                     TALGLVICVAVLGGITVLTELAWWIRLIHLSLAELLIGSVTAIVWLSFTCNILWNAPP
                     QQISTPNWAGKLWVISVGIFILIISGSYMVGAGAGSACSTWPLCRGELFPSGTAYAIH
                     MGHRYLAGLILIAAGYIGLSFIKEDGNIPGVKQASMILVHSILLQIVIGAILVWIGFD
                     PILKTIHLALATIIWMAVIYMVACILENHSRYSKG"
     gene            2000..2075
                     /locus_tag="QF838_05195"
     tRNA            2000..2075
                     /locus_tag="QF838_05195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2033..2035,aa:Pro,seq:cgg)
     gene            2123..3343
                     /locus_tag="QF838_05200"
     CDS             2123..3343
                     /locus_tag="QF838_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60112.1"
                     /translation="MSKNELSRSKLGIASWVVYDMGNTLFSAGIVGLVFPLWLTRDMA
                     GNDGTFGFSLSISMVIVFILSPVAGTISDVTKRKMPFLLFTTLVAIFATALIGVFNYE
                     ASIVLFCAAVILIHLANIFYNALLADMTTESNVGYIGGLGVGIGYIGAILAVVCGLLF
                     YDSEGPVFLFKLMALLMFLLVLPLFLSGHYFIGNTEITELRSVLLRALRQAFLSLWHA
                     RKKKVWAMFLFARFWYFSSIYAGSVFAVLYGVETVDLSEKEVQLILAVGIIFGIPSGA
                     VWGFLVDKYGSFLNLKINVMAWFLILGLAALLPVLDLPGELWWAVGVMSGVFMAGLYV
                     SERPFVLSMSAPDTVSEYFAVYNMVGRLAAITGPFAWGYISSTLGLGQVASITWLSIC
                     SLIGFLVLICTKLE"
     gene            complement(3346..4236)
                     /locus_tag="QF838_05205"
     CDS             complement(3346..4236)
                     /locus_tag="QF838_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015218219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HJO60113.1"
                     /translation="MKYAFYPGCVSRGACPELYSATIEVCRILDIELNEEALKGASCT
                     GAGVLQEKNLRLGDTLNARTFAMAEKSGNPLMTICSTCQGVMSQANERLLADPEYLAS
                     INEDLKEEGLEYKGTADPKHLLWVIVEDLGIDFLESKIVNKLNGLKLAPFYGCYIVRP
                     TKALGFAENSGRKQALEMVIESVGAEVVDFPGKTLCCGFPILTINPENSVNMVANHTI
                     DAKDRGADAMVTPCPLCHLNLDGYQPNAASARKREIDLPIIHLPQLLGLALGISPEKM
                     RLNKHIVSTKKLLSELVISP"
     gene            complement(4233..5189)
                     /gene="sdhB"
                     /locus_tag="QF838_05210"
     CDS             complement(4233..5189)
                     /gene="sdhB"
                     /locus_tag="QF838_05210"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HJO60114.1"
                     /translation="MEVKFNVRRYDPESTDAVDHFQEYLVDMDDSSTVLDGLIRIREE
                     VDGTLSLRCSCRSAICGSCAVRVNGEAGLACNTKIIDVLSLDGKTVTIEPAGNLPVIK
                     DLVVDFQPFWEKIKSVDPWLQPEGEEPEAEYLAPDEDMLHLAGVVSCILCGACVSDCT
                     VMEVDSNFLGPAALAKSYRFVGDPRDDSSQQRFKTLNGYGGVWDCTRCMKCVEVCPKG
                     VAPMDRIMALREQVMESGYKNTNGARHANEFTNSIKHSGWLDEKKLVIKSFGIFNIKA
                     MIGLIPLAIRSQRAGKVPPIFHKNIPGVENVRRIFEKVETKK"
     gene            complement(5211..6887)
                     /locus_tag="QF838_05215"
     CDS             complement(5211..6887)
                     /locus_tag="QF838_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJO60115.1"
                     /translation="MLDHDILIIGAGLAGMRAALSAKELGADVAVVSKVHPVRSHSNA
                     AQGGINAALTDRGDDWRDHAYDTIKGSDFLGDQDAIALMCQEAGSEVIAMEHMGAIFN
                     RDEEGRLGTRAFGGQREARTFFVADFTGQALLHVLYEQILKAGALVYEEWFVLSLIVR
                     DGKCGGAVMMDIRTGRIEVVRAKAVILAAGGLGRVFEPSTNALICTGDGMSLGYRAGA
                     SLMDMEMVQYHPTTLKGSGVLISEAARGEGAYLLNSENDRFMLKYAPEFKELASRDVV
                     SRCEQTEINEGRGLDGCVLLDVRHLGEKYISERLSQIRELAMDFANVDMVKEPVPIMP
                     GMHYQMGGVKANVDGETGVPGLYAAGEVACVSVHGGNRLGANSLLDTLVFGRRSGIHA
                     AEMVKSTQHVNVDDSAADDDKSNIQQLLANDKNESFGQIRLDMGTAMKEHFGVFRDEN
                     GMREGIEKIESVKKRAEDVYIADKGNIFNTNLLFTLELGFMLECAESIAVSAADRTES
                     RGAHTRTDLPDRDDVKWLKHILVSQNDGGGHELSYKDVVITDWEPVVRQY"
     misc_binding    7094..7398
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7493..9187
                     /gene="ilvB"
                     /locus_tag="QF838_05220"
     CDS             7493..9187
                     /gene="ilvB"
                     /locus_tag="QF838_05220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HJO60116.1"
                     /translation="MIKKGSQIVCESLLREGCDVVFGIPGGAILPLYQALPEYPELRH
                     ILTRHEQGASLAADGYARVTGKPGVAFATSGPGATNLVTGIASAQMDSVPVVIVTGQV
                     NSWTIGSDAFQETDITGITLPVTKHNFLVMDASEIASSIKEAFHIANSGRPGPVLVDI
                     PRNVFIQEIEYEEPGELNLPGYSPNFSGHSGQIRKAAHLINESERPVIVAGHGIIISK
                     AYDELKELAERAQIPVITTLLGISCFPEDHVLWTGWPGMHGMAYSSFAIEEADLIIAL
                     GMRFDDRITGDPSKFALNSKKIHIDVDPSEIGKNIAVDVPIVGDVKEVLTRMNTQIVA
                     ATHPAWLKRIDVLKKDHPSLDIPETSKLLARQVVKDLSEETKGGATIVTGVGQHQMWA
                     AQHYRFKKPCTFLTSGGSGTMGYEVPAAMGAQVADPDSTVWSIAGDGGFQMTLCELAT
                     IAENRLPIKFAILNNGFLGMVRQWQEIFFDSSYVSTAYTGNPDFVRLAEAYGIKGIKV
                     TDKTQVKTAITEAMEHDGPVLIDFIVEQEENVYPMIPSGMTVYDMIEEPVSEKVTT"
     gene            9184..9726
                     /gene="ilvN"
                     /locus_tag="QF838_05225"
     CDS             9184..9726
                     /gene="ilvN"
                     /locus_tag="QF838_05225"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HJO60117.1"
                     /translation="MKSSEKHTITALVEDRAGVLNRISSMFRRRGYNISSLAVGKSES
                     AGLSRMTFVVDGDAKTVEMVVKNLHKLVEVIKVADISEENAVSRELALIRVKCDVATR
                     SEIMQIVDIFRAKIVDVSQDTLIVEVTGNEDKVNSLVDLLDTFGVFEVMRTGTVAMSR
                     GASFEQDKAVTSPRILENAS"
     gene            9756..10754
                     /gene="ilvC"
                     /locus_tag="QF838_05230"
     CDS             9756..10754
                     /gene="ilvC"
                     /locus_tag="QF838_05230"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HJO60118.1"
                     /translation="MAKMYYESDADMSLLEGKVVGIIGYGSQGHAHALNLKDSGVDVC
                     VGLYEGSKSWSKAESEGLKVATVAEVAAQSDVIMVLIPDHLQASVYNESILPHLLPGK
                     TLMFAHGFNIHYDAINPPDNVDVSMVAPKAPGHRMREVYTKDSGVPGLLAVHQDSTGN
                     AHALGLAYSRGVGCTRAGVLDTTFKEETETDLFGEQAVLCGGVAALVKAAFETLIEAG
                     YQPESAYFECMHELKLIVDLFYQGGMEYMRYSVSDTAEYGDYTRGPVVVDENVKKNMR
                     KVLDQIQDGTFAKEWITENDEGRPTFNRLREENADHQIEEVGKELRGMMSFLSDSD"
     gene            10938..12470
                     /locus_tag="QF838_05235"
     CDS             10938..12470
                     /locus_tag="QF838_05235"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJO60119.1"
                     /translation="MSQEKVTIFDTTLRDGEQSAGIGLTTQEKLEIAKQLERLGVDVI
                     EAGFAAASPGDLEAVQKIASEVRTPKIASLARCYIDDVDKAWEGVKYAARPRLHVFIS
                     SSDIQIMNQLRKNPEEVLDIAVASVERAKTYCDDVEFSPMDATRTEPEYLYQMLEAVI
                     NAGATTVNIADTVGYIIPSEFAERIEDIKRNVPNIDKAVLSVHCHNDLGLSVANSLAA
                     VRAGVRQVEGCINGLGERAGNASLEEIIMAIETRNDFFDVSTNIDTTQIYRTSRLVSD
                     ITGFPVQPNKAIVGANAFRHASGIHQDGVLKDSSTFEIMDPKSIGWPSNSFVLGKLSG
                     RAGLRARLEELGFHLQQEELNEVFESFKELADRKREVTDQDLETLLSNSRRTADIPTI
                     YDLQHVQVSTGDHGVPTATVKIRNAEGEEITDAATGTGPVDAVYMAINRVIGIENRLT
                     EFRVDSVTEGIDAIGDVTIRIELQGDAFVGRGSDTDIIVASAKAYMNALNRALAANPN
                     VG"
     gene            12537..13937
                     /gene="leuC"
                     /locus_tag="QF838_05240"
     CDS             12537..13937
                     /gene="leuC"
                     /locus_tag="QF838_05240"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HJO60120.1"
                     /translation="MAARTMFDKIWEAHLVKEVEGQPSLLYVDLHLVHEVTSPQAFDG
                     LRMAERPVRRPDLTVAVADHNTPTWDLSLPITDPIAKQQLDALTQNCEEFGVTLHDRF
                     SPLQGIVHVIGPEQGHTQPGKTIVCGDSHTSTHGAFGAFAIGIGTSEVEHVLATQTLR
                     QFKPKTMEVRVEGPLLSGISAKDVVLSIIGEIGTAGATGHVIEYTGEGITSLSMEGRM
                     TVCNMSIEAGARAGMIAPDQTTFDYMRGRDNVPQGKDFEKAVSEWGKLSTDVGAEYDR
                     VVIVKSEDLEPHVSWGTNPGMVGSISSRIPNPDDYSDEGERESIERALAYMDLEPGTR
                     IQDIPVDRIFIGSCTNSRIEDLRAAARVVEGKKVRENIYAMVVPGSNAIKRQAEEEGL
                     DIILTEAGFDWREAGCSMCLGMNPDILQPGERCASTSNRNFEGRQGRGGRTHLVSPEM
                     AAAAAIAGHFVDIREW"
     gene            13958..>14411
                     /gene="leuD"
                     /locus_tag="QF838_05245"
     CDS             13958..>14411
                     /gene="leuD"
                     /locus_tag="QF838_05245"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HJO60121.1"
                     /translation="MEPFVSLNAKLAPLNRVNVDTDQIIPKQFLKRVERTGFGQFLFN
                     DWRLMPDGSENKDFILNRKGYRDAEILVSGRNFGSGSSREHAPWALQDYGFKCIIAPS
                     FGDIFFSNCFQNGVLPVVLPEDTVAQIIENSENDPEYRINIDLESQRVW"
BASE COUNT         4094 a         2915 c         3340 g         4062 t
ORIGIN      
        1 gctatatcaa gatgaaactt tccgcaacca ttaccacgaa aagcaacgca aggaaggcca
       61 tggaatagag gtaaataggc atagccaatt cgatgttgtc ccttcgcttc aacagccagg
      121 ttgaccatat gaatcctgca cataacaata ctgaagaaat tccaaaaatg attgagagct
      181 cttccacaaa atacgttgct gccagcaata aaactagcaa tatccaagtg taaataaata
      241 tttgagtttt tgtcgctgca atgccagaaa ctactggcaa catgggaata cccgcatttt
      301 cgtaatcatc tttcaatatt aaggaaaggg cccaaaaatg gggtggtgtc cagaaaaaca
      361 cgattaggaa tagaaaaatg ggagccgggt ccataatgct attggtaacg gctacccagc
      421 caacggtagg tggaatggag ccagcggtcc caccgattac tatgttttgg ggggtcgttc
      481 ttttcagtac cattgtgtaa acaaatatat aaaacaaggt ggcggataac gtcagtaatg
      541 cgctcagtag gttggtgtat ctgtagagaa ccgcaaaggc caccaagttc agtagaatcc
      601 caaaaatcaa agcgttagta ggggggactg cacctgatgc cacaggtctg gtggtagttc
      661 tcttcatctt tatgtctata tcgcggtccc agaaatgatt tagtgaattg gcgccaccgg
      721 ctgctaaggt gccacctatt aaaacggctg cagcgatagt caggtcagga aacccgtctg
      781 aagcgagaaa tagcccacct accgtggtta tcaatagcag gacgattatt ttcggttttg
      841 ttaatgctat ataggacgaa atcatacatc agccttttga atatctggaa tgattttcca
      901 atatgcaagc caccatgtaa atgactgcca tccagattat tgttgccaag gccaggtgga
      961 tagttttcag tatagggtca aaaccaatcc atactaaaat tgcgccaata acgatttgca
     1021 acaggatact atgaactaaa atcatggagg cctgtttcac accggggata ttcccatctt
     1081 cttttatgaa agagagacca atatatccag cagctattag aatcagacca gctagatacc
     1141 tgtggcccat gtgtatggca taggcggtgc cggaagggaa tagttcccct ctgcacaatg
     1201 gccaggttga acatgcggaa cccgctcctg ctccaaccat ataggaacca gagatgatga
     1261 gtatgaagat acctacagaa atcacccaca atttccctgc ccaattcgga gtagatattt
     1321 gctgaggagg agcgttccac aatatgttgc aagtaaatga caaccagaca attgcagtaa
     1381 cagatcctat caataattca gcgagcgaaa gatgaattaa tcttatccac caggctaatt
     1441 cagttaggac tgttattcct ccaagtacgg caacacatat gacgagtccc aatgcagttg
     1501 cagcaaggaa agtaacgatt ttttcttctc taaaccgaag ccagcataat aggaaaagtg
     1561 ttcctacaag aaccattaac aaagatgcat ttaggcggtg ggaatattca acaagagtat
     1621 gaaattcaaa gggaggtata atttgcccat gacatagggg ccaatcaggg catcctaatc
     1681 ccgactcact ggatctgaca tatcccccca gagttacctg actgaaagca gatatcaacg
     1741 tgataattga tagccatttg agggtgtttc tagggttagg tcggggtgta gttgtcctgt
     1801 tcaataagga ggtcaacctc tcttaattcg cggctatggt aaccggatta acactatgtg
     1861 ctttttttca cttattaacc gtagcacatc aattttgagg ggtcaaaaaa atccaaccat
     1921 cctaatacta tttgttaatt actatatcca atgtatattg ccctattttg taaaaagtct
     1981 ctaaaattga tttgaatatc ggggcgtagc gcagttggta gcgcgccacg ttcgggacgt
     2041 ggaggtcgct ggttcgagtc cagtcgcccc gaccaattcc ttagaaatcc tataaacttg
     2101 gaagttatga gtaacttcta taatgtcaaa aaatgaactg tctcgatcca aactgggtat
     2161 tgcttcatgg gtcgtttatg acatggggaa tacgttgttt tcggctggta ttgttggtct
     2221 ggtttttccg ttatggttga ccagagatat ggcaggaaat gacggcacat ttggattttc
     2281 actttctata tcgatggtca ttgtgtttat actttcaccg gttgccggca caatttctga
     2341 tgtcaccaaa cgtaaaatgc catttttact tttcactact ttggtcgcta tttttgccac
     2401 agcgttaatt ggtgtattca actatgaagc ttccatagtt cttttttgtg ctgccgtcat
     2461 tttgattcat ttagccaata tcttttacaa cgcgctgttg gcagacatga ctaccgaatc
     2521 aaatgtcgga tatattggag ggttaggggt tgggattggt tatatcggtg ctatattagc
     2581 tgtagtttgt ggattgttgt tttatgattc tgaagggcct gtttttttat tcaaattaat
     2641 ggccctattg atgtttcttt tagttttacc gctgttttta tctgggcact attttatagg
     2701 aaataccgaa attactgagc tccgatcagt tttgttaagg gctttaagac aggctttcct
     2761 gtctctatgg catgccagaa agaaaaaagt ttgggcaatg ttcctctttg cgcgtttttg
     2821 gtatttttcc agcatatatg ccggttcagt atttgctgtt ctgtatggag tcgaaaccgt
     2881 cgatttgtca gagaaagaag ttcaattgat attagccgtg gggattattt ttggtatccc
     2941 gagtggtgcc gtctggggat tcttggtgga taagtacggt tcttttttga atttgaaaat
     3001 taatgttatg gcttggtttt tgatattggg attggctgcg cttctgccag tacttgatct
     3061 cccgggagag ctatggtggg ctgtaggggt gatgagtggc gtattcatgg cgggtctata
     3121 cgtgtcagaa agaccgtttg ttctttccat gtctgctccc gataccgtct cggaatattt
     3181 tgcagtgtat aacatggttg gtaggctcgc ggctattaca ggaccctttg cgtggggtta
     3241 catatcatcg actttaggat tgggacaagt ggcctcaatt acgtggttaa gtatatgctc
     3301 acttatagga ttcctagtat tgatctgtac caagctggag tgaatttagg gggatattac
     3361 aagctcgctc aaaagtttct ttgtagacac aatatgctta ttcaagcgca ttttttccgg
     3421 ggagataccc agtgctaaac ccaataattg aggcaggtgg attataggta aatctatttc
     3481 acgttttctt gcggatgctg catttggctg gtacccatcc aaattgagat gacatagagg
     3541 gcatggggta accatggcat cggcaccccg gtctttggca tctatggtgt ggtttgcaac
     3601 catattgacc gaattttctg ggtttatagt taaaataggg aaaccacagc acagagtctt
     3661 gcctggaaaa tctactactt cagcccccac actttctata accatttcca atgcttgttt
     3721 gcgaccagag ttttcagcga accctaatgc ttttgtaggc ctaacgatat agcaaccgta
     3781 aaacggagcc aatttcaacc cgttaagttt gtttactatt tttgattcca ggaaatctat
     3841 tcctaaatct tctacaatta cccacaacaa atgcttaggg tctgccgttc ctttgtattc
     3901 aaggccttct tctttcaagt cctcattaat tgatgccaaa tattcagggt ctgccagaag
     3961 tcgttcgtta gcctgagaca taactccctg gcatgtgctg catatagtca tcaaaggatt
     4021 cccggacttt tctgccatag caaaggttcg agcattaagg gtatcgccaa gcctcaggtt
     4081 tttttcctgt aaaacgcctg caccagtaca agaagcaccc ttcagcgcct cttcattaag
     4141 ttcaatgtct agtatcctac atacctcgat agtggcggaa tataattctg ggcatgcgcc
     4201 tcttgatacg catcctgggt aaaaagcata tttcatttct ttgtctctac cttttcaaag
     4261 attcttctaa cgttttctac tcccggaata tttttatgga atataggtgg aaccttgccg
     4321 gcacgctgag atctaatcgc taacggaatt agtcctatca ttgcctttat attaaatata
     4381 ccgaaacttt ttatcaccaa ttttttttca tcaagccacc cactgtgctt tatagagttt
     4441 gtgaactcat tggcgtgcct agcaccgttg gtatttttgt atcctgattc catcacctgt
     4501 tcacgcaatg ccattattct gtccattggg gctacccctt ttggacaaac ttcgacacat
     4561 ttcatacacc tagtgcaatc ccacacccct ccatacccat taagagtctt aaatcgttgt
     4621 tgggatgaat catctcttgg atcccccaca aatctgtatg acttggcgag cgctgctggg
     4681 cctagaaagt tggaatctac ttccataact gtacagtctg agacacaggc tccacagagt
     4741 atgcaagata caactcctgc cagatgaagc atatcttcat ctggggctag atattctgcc
     4801 tcgggctcct ctccttctgg ttgtaaccat gggtccactg acttaatttt ttcccaaaat
     4861 ggctgaaaat ctactacaag atcttttatt actggcaggt taccagcagg ttctatggtt
     4921 accgttttcc catccaaaga caatacatct atgatttttg tgttacacgc aagaccagct
     4981 tcaccattaa ccctcaccgc acaggatcca catatggcac tcctgcagga gcatctcaaa
     5041 ctcaatgttc catcaacttc ttctcggatg cggattaaac cgtcaagaac tgtggatgag
     5101 tcatccatat ccactaggta ttcttggaaa tggtctactg catcagtcga ttctggatca
     5161 tatcgtctaa cgttaaattt aacttccatt aacaaaaatc cttttggtta ttagtattgt
     5221 cttactactg gttcccaatc ggtgataaca acgtctttat aggatagttc atgtccgccg
     5281 ccatcatttt gagaaaccaa gatgtgcttt aaccacttca catcatccct atctggcaga
     5341 tcagttcggg tatgagcccc tctgctctct gttcgatctg cagcactcac ggctattgat
     5401 tccgcacatt ccaacataaa acccaattct aaggtgaaaa gaagattggt gttaaagatg
     5461 ttgcctttat cggctatata tacatcttca gctcttttct tcactgattc aattttttct
     5521 attccttctc gcataccgtt ttcatccctg aacaccccga agtgttcttt catggcagtt
     5581 cccatatcta aacggatttg accaaatgac tcatttttat cgtttgccaa taattgttgg
     5641 atgttgcttt tgtcatcgtc ggccgctgaa tcatctacat tcacgtgttg tgtggatttg
     5701 accatttcag ccgcgtgtat cccggatctc ctaccgaaaa caagtgtgtc tagtagcgaa
     5761 ttggctccta gcctattgcc cccgtgcaca ctaacacacg ctacctcacc agctgcgtac
     5821 aacccaggta ctccagtttc accgtcaacg ttcgctttca cccctcccat ttggtaatgc
     5881 ataccaggca ttatgggcac tggttcctta accatgtcta cgttggcgaa atccattgct
     5941 aattcacgta tttgggataa tcgctcgctt atgtatttct ctccaagatg tctcacgtca
     6001 agcagcacac atccgtctag tcctcgacct tcgttgattt cagtttgttc acatcttgac
     6061 acaacatcac gggaagctaa ttccttgaat tctggtgcgt atttgagcat gaatctatca
     6121 ttttcagaat tcagtagata tgcaccttcg ccacgagcgg cctcacttat taaaacccca
     6181 ctacctttta aggttgttgg atggtactgc accatttcca tatccattaa tgaagcacca
     6241 gctctgtatc ccaggctcat tccatcaccg gtacatatca atgcgtttgt acttggttca
     6301 aatactctac ccaagcctcc agctgcaaga atcactgctt tggcacgaac aacctcaatt
     6361 cttccagtac gaatatccat cattactgcc ccgccgcatt tgccatccct tacgattaag
     6421 gaaagtacaa accattcctc atataccaag gcacctgctt tcaaaatctg ttcgtataat
     6481 acgtgtaaaa gagcttggcc agtgaaatct gctacgaaga aggttcttgc ttctcgctgg
     6541 ccaccaaaag ctctagtgcc aagccttcct tcttcatccc tgttgaatat agcgcccatg
     6601 tgttccattg cgattacttc gctacctgcc tcttgacaca ttagtgcaat cgcgtcttga
     6661 tctcccaaaa aatcgcttcc ctttatggtg tcgtaagcat gatctcgcca gtcgtcccct
     6721 ctatctgtca aagcggcatt tattccgcct tgtgcagcat tagaatggct tctgacggga
     6781 tgcactttgg ataccacagc aacgtcggcc ccaagttctt tagccgacag agcggctctc
     6841 ataccagcta ggccggcacc gattatcaaa atatcgtggt caagcatatt acctgaatta
     6901 acccccggat tattaataaa gactcaaaga caaaagatag cataggacct ctgaagggac
     6961 aacaaatggt tcaattccat cagttctgta ctctcttttt acatcaagca taaggtcagt
     7021 tagaatgtaa actttttgtt tgacccctat tgccatgagg tgttagaatg caacgtcttg
     7081 gaaaaaggta aataaatttg ctgtgacggg gagtagtaac agaggggttg tgagagagag
     7141 agtcggacat tacagctgga agtctgatac acaacgactg tgaactcgcc cttgagcatc
     7201 tgtcttgaaa attaggagtc agtaatgcag ttagtgatga gaaaactcaa cgcttctgca
     7261 tgctatattg tggctcacta acataaagta gggacaggcg gtagccacgt tatagctcaa
     7321 cgagtggtcc tgttgaagtg atttacaggg caatttgggt ggtaccacgg gagtggtttt
     7381 tgttatcccg tcccagctta agggcgggat ttattttttt cgagacccca gcccttttaa
     7441 tcgaaataag aattcgccaa ttatttatat gtagattaat taaggaagtt gaatgataaa
     7501 gaaaggatca cagattgtct gtgaaagttt gctcagggaa ggctgtgacg tagttttcgg
     7561 gatacctggt ggagcgattt tgcctttata ccaggcctta ccagaatacc cagaactccg
     7621 tcatatattg acacgacacg aacaaggtgc ttctttagca gctgacggat acgctcgagt
     7681 tacgggtaaa cctggagtgg cttttgcgac atcgggtcca ggggctacaa accttgtgac
     7741 aggaatagct tcagcccaaa tggattctgt accagtggta attgtaactg gacaggtaaa
     7801 tagttggact atagggtcag atgcctttca ggaaactgat atcacaggga taaccttacc
     7861 tgtcacaaaa cacaactttt tagttatgga tgcctcagag atagcttctt cgattaaaga
     7921 agcatttcat atagcaaact caggccgacc aggaccagta ttggtagata tacctaggaa
     7981 cgtatttata caggaaattg aatacgaaga accaggagaa ttgaatttac ctggatatag
     8041 tcctaacttc tccgggcatt ctggtcaaat acgaaaggca gctcatttga ttaatgaatc
     8101 agagagaccg gttattgttg cgggtcacgg aatcataata tcaaaggcat acgatgagtt
     8161 aaaggaattg gctgagaggg ctcaaatacc tgtcattacc acgctgctcg gaattagctg
     8221 ttttccagaa gaccatgttc tatggactgg ctggcctggc atgcatggaa tggcatattc
     8281 tagtttcgca attgaggaag cggatctcat tattgcgtta ggtatgcgtt ttgatgaccg
     8341 aataactggg gatccatcta agtttgcctt aaattcgaaa aagatacata tagatgttga
     8401 tccttcagaa ataggaaaaa acattgcagt tgatgtgcct atagtgggag atgtaaaaga
     8461 agttctgact cggatgaata ctcagattgt ggctgcgacc cacccggcat ggttgaaaag
     8521 aatagacgtt ttgaagaagg atcatccttc attggatata cctgagactt ctaagttatt
     8581 ggcccgacag gtagttaaag acctttctga ggaaaccaaa ggaggagcca cgatagtgac
     8641 aggtgttggc caacatcaga tgtgggctgc acaacattat cgattcaaaa aaccctgcac
     8701 gtttcttacc tcaggtggtt caggaacgat ggggtatgaa gtacctgctg ctatgggtgc
     8761 acaggttgct gaccctgatt ctaccgtttg gtctatcgct ggtgacggtg gtttccagat
     8821 gacactttgc gaattagcaa ccatcgcaga aaacaggcta ccaattaaat ttgcaatatt
     8881 gaacaatggg tttttgggaa tggttaggca gtggcaagaa atattcttcg atagttcata
     8941 tgtctcaaca gcctacacag gtaatcctga cttcgttcgt ttggccgagg catatggcat
     9001 aaaaggtatc aaagtaacgg ataaaacaca ggtaaaaacg gcaattactg aagctatgga
     9061 acatgatggt ccggtgttga ttgacttcat agttgagcaa gaggaaaacg tataccccat
     9121 gattccatca ggcatgacag tttatgacat gatagaggaa ccagtttcag agaaggtgac
     9181 aacttgaaat cgtcagaaaa acacaccata acggcattgg ttgaggatcg tgctggtgtc
     9241 ctaaaccgca tatctagtat gttcaggagg cgtggttaca acatatcaag tctggcagta
     9301 gggaagagcg aatccgctgg tttatccaga atgacattcg tagttgatgg ggatgccaaa
     9361 acagtcgaaa tggtcgttaa gaacctccat aagttggttg aagtcattaa agtagctgat
     9421 atatctgaag agaatgcggt gtcgagagag ttagccctaa taagggtcaa atgcgatgtt
     9481 gcgactcgta gcgaaatcat gcagatcgtt gatatattta gggccaagat agtagatgtc
     9541 tcgcaggata ccttgattgt cgaggtaacg ggtaatgagg acaaagttaa ttcccttgta
     9601 gatttactgg atacttttgg ggtttttgag gtgatgagaa ctggcacagt ggctatgagt
     9661 agaggggcct cctttgagca agataaggcc gtgactagcc caaggatttt agagaatgca
     9721 tcttaaaaaa agaatattgt attaggagaa tttatatggc gaaaatgtat tatgaatccg
     9781 atgcagacat gagtctgtta gagggtaaag tcgtaggtat tattggctac ggtagtcagg
     9841 gccatgctca tgcgttaaat ctgaaggatt ctggggtaga tgtatgcgta gggttgtatg
     9901 aaggtagtaa gagttggtct aaggcagaat cggaaggcct aaaggtagcc acagtggcag
     9961 aggttgctgc ccaatcagac gttattatgg tattgattcc tgatcatctt caggcttcgg
    10021 tttacaatga atccatatta ccccaccttt tgccgggcaa gactttaatg tttgctcatg
    10081 ggttcaatat tcattatgac gcgataaatc cacctgataa tgtcgacgta tccatggtcg
    10141 cacctaaagc acctgggcat aggatgaggg aagtttatac gaaagactct ggggtgcctg
    10201 ggttacttgc ggttcatcaa gattcaactg ggaatgccca tgcgttgggc ttggcttatt
    10261 caagaggcgt aggttgcact cgggcagggg tacttgacac cacttttaaa gaagagactg
    10321 agacagacct tttcggggaa caagctgttt tgtgtggtgg ggttgcagcc ttggtaaaag
    10381 ctgcatttga gactttgatt gaagcggggt atcaaccaga gtccgcttat ttcgaatgta
    10441 tgcatgagtt gaaacttata gtggatttat tctatcaagg tggtatggaa tacatgaggt
    10501 attcagtgag tgataccgct gaatacggag attacacgag gggaccggta gtagtagacg
    10561 aaaacgtgaa aaagaatatg cgaaaggtac tggaccaaat tcaagatggt acgtttgcta
    10621 aagaatggat cacggaaaat gatgagggaa ggccaacatt taataggctg cgggaggaaa
    10681 atgcggatca tcagatagaa gaggtgggta aagagcttag aggtatgatg tcctttctct
    10741 ctgactcaga ctaggattgg ttctatatat gaataatatg aaatgtaagt cgatagacga
    10801 ggtatcctgc gtagtccgcc atgttgattt ggtcttggca aggctgaatc aggcggacta
    10861 cggaatcctg cgaatttgca aaggcaatta aggcagataa acctttagta atacttgact
    10921 aataaaagga gaccgaaatg tcccaagaga aagtcacaat ttttgatact accctgagag
    10981 atggtgagca gtcagcgggc ataggactta caacccaaga gaaactagaa attgccaagc
    11041 aattggagag attgggagtt gatgtaatag aggctggatt tgcggcggca tccccaggag
    11101 acttagaggc ggttcagaaa atagcctcag aagttcgaac tccaaagata gcttcccttg
    11161 ctaggtgcta tatagacgat gtagataagg cttgggaagg tgtcaaatac gcagctagac
    11221 ctcgtcttca tgtttttatt agcagttctg acatacagat aatgaatcaa ttgcgaaaga
    11281 acccagagga agttcttgat attgctgtgg cttctgttga gagggcgaaa acttattgcg
    11341 atgacgtgga attttctccc atggatgcaa ctcgtactga acccgagtat ttgtatcaaa
    11401 tgctcgaagc agtcataaat gccggtgcca ccacagttaa catagcagat actgtcgggt
    11461 acattattcc gtcagaattt gcagaaagaa tagaagacat taaaagaaat gtacccaaca
    11521 tagataaagc agtattgagt gttcactgcc acaatgatct tgggctttct gtagctaata
    11581 gtctggctgc agtgagagca ggggttaggc aggttgaagg ctgcatcaac ggtcttggag
    11641 aacgggcagg aaatgcatcg cttgaagaga taattatggc cattgagacg agaaatgatt
    11701 tttttgatgt ctcgaccaat atagacacta ctcagatata taggacgagt aggttggtaa
    11761 gtgatatcac cggtttccca gtacaaccaa acaaagcgat tgtgggagct aatgcatttc
    11821 gacacgcttc cggtatacat caggatggtg tcttgaagga tagtagcaca ttcgaaatta
    11881 tggatcctaa atccatagga tggcctagta actcatttgt tttagggaaa ctgagtggac
    11941 gggctggtct cagagctcgg ttagaagaac ttggctttca tctgcagcaa gaggaactca
    12001 acgaggtttt tgaatcattt aaggagctag cagatcgtaa aagggaggtc acagatcaag
    12061 atcttgagac tttactatca aacagtcgta gaacagcaga tattccaacc atatatgatc
    12121 ttcagcatgt tcaagtatcc actggtgacc atggtgtacc aacagcaaca gtgaaaatca
    12181 gaaatgcaga aggggaagaa atcacagatg ctgctactgg tacaggacct gttgacgcag
    12241 tttatatggc tataaatagg gtaataggga tagagaatag actcactgaa tttcgagtag
    12301 attcagttac agaaggaata gatgccatcg gagatgtgac catcaggata gaactccagg
    12361 gggatgcctt cgtaggaaga ggcagtgaca cagacattat agttgcaagt gctaaggcct
    12421 acatgaacgc tttaaaccgt gctttggcgg ccaatcccaa cgtcggttaa tacaaaactt
    12481 gttgtgtttt gatgataaga gaaatcaaat ttctaaaatc caatatggag tattaaatgg
    12541 ctgctaggac catgtttgat aagatatggg aagctcatct agtgaaggaa gtagaaggtc
    12601 aaccctcgct tttatatgta gatcttcatc ttgttcacga agttacttct ccacaagctt
    12661 ttgacgggct taggatggct gagagacctg taaggcgacc agatttgacg gtcgcggtgg
    12721 ctgaccacaa cactccgacg tgggatctgt ccctaccaat aactgatccg attgctaaac
    12781 aacaattgga tgctctgact caaaattgtg aagaatttgg agtgactctt cacgataggt
    12841 ttagtccttt acagggcatt gttcatgtta tagggccgga acaaggccat actcaacctg
    12901 gcaagacaat cgtttgtgga gacagccaca cttcgacgca tggtgctttt ggagcgtttg
    12961 ctataggaat aggtacatcc gaagttgaac acgtgttggc gactcagaca cttaggcagt
    13021 tcaaaccgaa aaccatggaa gttagagttg agggaccttt actatcaggt atttctgcaa
    13081 aggatgttgt gttaagcata attggagaaa ttggcacagc aggagccaca ggtcatgtaa
    13141 tcgaatatac gggagaaggc ataacctctt tgtccatgga aggccggatg actgtgtgta
    13201 atatgagcat cgaagctggt gccagggcgg gtatgatagc acccgaccaa accacattcg
    13261 attatatgag aggacgggat aatgtccctc aaggtaaaga ttttgaaaag gcagtaagcg
    13321 agtgggggaa actttccact gatgtcggtg cagaatatga cagggtggtt attgttaagt
    13381 cggaggacct agagccccat gtcagttggg ggaccaaccc aggtatggta ggttcaatat
    13441 catcccgcat accaaatccc gatgactatt ctgatgaagg ggaaagagaa tctatagaaa
    13501 gagccttagc ttacatggat ttggaaccag ggactagaat acaagatata ccagtggatc
    13561 gtatcttcat cggttcttgt accaattcaa ggatagaaga cttacgggct gctgcgcgag
    13621 tagtcgaagg gaaaaaagtt agagagaata tttatgccat ggtcgtgcct ggttctaatg
    13681 ccataaaacg gcaggcggaa gaggaaggac ttgacataat actcacggag gcagggtttg
    13741 attggagaga ggctggttgt tctatgtgtc tggggatgaa tcctgacata ttacaaccgg
    13801 gggaaagatg tgcttctact tcaaacagaa attttgaagg aagacaaggt agaggaggaa
    13861 ggacccattt agtcagccca gaaatggcag cagcagcagc gatagcagga catttcgtcg
    13921 atatacgtga atggtaaaaa accaaggaag aatcaaaatg gaacctttcg tttccctaaa
    13981 tgcaaaacta gctcctctta acagggttaa tgtagatacc gatcaaatca tcccgaaaca
    14041 atttttaaaa cgtgttgaga gaacaggctt cggtcaattt ttgttcaacg attggagact
    14101 catgccagat ggctctgaga acaaagattt tattctgaat cgaaaaggat atcgcgatgc
    14161 tgagatactg gtgagtggaa ggaatttcgg atcgggttca tctcgagagc atgctccatg
    14221 ggccctgcaa gactacggat ttaaatgtat tatcgctcct agctttggag atatattttt
    14281 tagtaactgt tttcaaaatg gtgttttacc agtggttttg cctgaagaca cagtggccca
    14341 aataattgaa aattcagaaa atgatccaga atatagaatt aatatagatt tggagtcaca
    14401 acgggtatgg g
//
LOCUS       DASCNS010000147        13422 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001176090, whole genome shotgun sequence.
ACCESSION   DASCNS010000147 DASCNS010000000
VERSION     DASCNS010000147.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13422)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13422
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001176090"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="QF838_05250"
     CDS             <1..635
                     /locus_tag="QF838_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015922.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HJO60122.1"
                     /translation="LDDVEHIRPSGLIIMNLKGDDELVSVKLAKTNDDIIFISEQGMG
                     IRFSVNDLPIRRRTAGGVKGMSLRTGDRVVSMDVGNDDSKLLVVSKLGRGKVNPLSEY
                     RRQGRGGLGLRAFKLTKNTGLIADAQIVDETNEVYLVSENAQVMRTDLSEIRSLTGRI
                     TQGVTIFKPRAGDSVSSIACVGDFELDEDEDPKQTDAKASKNGKNSKNKK"
     gene            complement(747..1661)
                     /locus_tag="QF838_05255"
     CDS             complement(747..1661)
                     /locus_tag="QF838_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016115.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HJO60123.1"
                     /translation="MAGRNIPSKSEVDSYLEDTNWGRWGDKSSAGAMNLITDEKRLEA
                     VSLAKIGRTVSLSRPLPVTPAGDNPRPAQHYMHKEERPFEGGAAMDYYGVFYHGTATT
                     HIDALCHVWDKNGMWEGKTPDDVIKFGGANFGTVEEWKDGILTRGILLDVPKHRNKPY
                     VTLSEPVHGWELQDIATEQNVEIKSGDAVFVYSGRDKYAADNNGRWGTQNGRPGLHAS
                     CLPFVKSNNISILGWDMMDASPNEYEIPWTVHGVIFAYGVALVDNAYLEPLSNVCAEE
                     GRYEFMLSLNPLYVQGGTGSPANPIAIF"
     gene            complement(1709..2602)
                     /gene="garR"
                     /locus_tag="QF838_05260"
     CDS             complement(1709..2602)
                     /gene="garR"
                     /locus_tag="QF838_05260"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HJO60124.1"
                     /translation="MARGEIGFVGLGIMGRPMVRNLIKADYAVTVYDIVAEAVEEMAT
                     EGATPASSAAEVASKTPIIITMVPDSPQSEAAILGPGGVLEGASKGDTVIDMSSIAPA
                     SSKKIGGACDAAGVNFLDAPVSGGETGAIEGTLAVMVGGEKEVFEKNYDMLTAMSGSI
                     VLCGGYGAGNTTKLANQIIVAGNIAALGEALVLAKKSGIDPKVVFEAIKGGLAGSTVM
                     NTKGPMMLEGNFDPGFRIVLHQKDLHNAILTGKDVGVPLMVTAMVQQILGSLINDGKG
                     NSDHSAIANFMEDMAGVTIAD"
     gene            2852..3544
                     /locus_tag="QF838_05265"
     CDS             2852..3544
                     /locus_tag="QF838_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HJO60125.1"
                     /translation="MSELTQNEIDSVATMQTCAVSNAIESLNIRSRTEGFMTPDIKSM
                     FPDMGSMAGFAVTAVIKASTPPSDNMNYSRVEWVDEILKIPGPRVIVMKDLDHPFVIG
                     SFWGEVQSAIHSSLDCVGVVTDGGVRDLDEMQEWGFNAFASTALVSRANVHLVEANIP
                     VTVGGVTVNPGDLLLGDKHGVISIPKEIAKDLPDAIAKVEAGEREIIDVCKAQNFSPE
                     KLKDILRQRYGG"
     gene            3563..4543
                     /locus_tag="QF838_05270"
     CDS             3563..4543
                     /locus_tag="QF838_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HJO60126.1"
                     /translation="MASPKVVFLTGLKEELIDEVISYSPTDYQIVVLDKSSTEQQQID
                     EVRDADFLLCYGQDPSNAVIRSLEKCRLVQLLAAGYDRMNLELLSELEIPCANNGGAN
                     SWAVADQALLLMLAIYKQLLASDNATKGGRWSEPITGQNTFEMADKKVGILGIGNIGR
                     QVAKRVQGFDAEVQYFDLYPLDEKVAEELNVKYVSLEELFRTSDIITCHTPLTKETKH
                     IVNSNTLAMMKPTAILINTSRGPVVDETALISALEEGLIAAAGLDVFEKEPVDPDNPL
                     LGMDNVVATPHMAGTTWDTWARRANFGFENMERVRQGEAPQAVVRNFDSS"
     gene            4566..5627
                     /locus_tag="QF838_05275"
     CDS             4566..5627
                     /locus_tag="QF838_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HJO60127.1"
                     /translation="MMGQGVRLRLEKNEENLNLYACRSAYSRGRRQIEDPSPIRTEFQ
                     RDRDRILHSKAFRRMKHKTQVFISPVGDHFVTRLTHTLEVSQIARTIARALNLNEDLT
                     EAIGLGHDMGHTPFGHIGEDELGKLSPLGFRHNVQSVRLVEELEKNGKGLNLTEEVRE
                     GMLHHSKPKGDFMSSNLVEGIGLEAQVIRISDAIAYLNHDLGDAFRAGVLKEADLPHE
                     TKEFLGRRHSERVDRMVMDIVCTSSRLLEDTSLTSRPTISMSISMRDAINVLRDFMFE
                     RVYIPVDSGPEGKAARLIINVLYEALDSNHNLIPNEFLEIGETVDHSIADYISGMTDG
                     FAIRTAESLSPGAGQVFAS"
     gene            5655..5921
                     /locus_tag="QF838_05280"
     CDS             5655..5921
                     /locus_tag="QF838_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60128.1"
                     /translation="MTESTNPVDRLKTRLVFKNIDHIQEHLEAMQRDPHGLEYRPWKL
                     EVDNIWKKIFADINEMSEESQKTVLDAMRDVWVSYITHYGAVEG"
     gene            5943..6284
                     /locus_tag="QF838_05285"
     CDS             5943..6284
                     /locus_tag="QF838_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60129.1"
                     /translation="MSLSFNPNMEQARRRSGLAHRVLVKLKTMGLSDDHDDSLAILCT
                     DIGDLWSSQLVFLEILNRFLEESDNWDSIGDDFADMLSDVEHISWHIDSLKKPLEILA
                     QYSYSQSKNTE"
     gene            6293..8086
                     /gene="dnaG"
                     /locus_tag="QF838_05290"
     CDS             6293..8086
                     /gene="dnaG"
                     /locus_tag="QF838_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HJO60130.1"
                     /translation="MSEIDEIKSKLDVVDIVSEKVRLQRAGRNYKANCPFHSEKTPSF
                     IVDPGRQSWRCFGSCGVGGDVFSFVMKTENMEFPDALRMLAQRTGVELRGYKKNESRD
                     SYFEINKIALDFYKDALLTDEAHSARIYLTSRGVDEGSIEDFGLGYSPRGRDSLKSHL
                     LFHDVDLETAVECGLLNKFEDGTTRDFFWGRLMFPIFDRLGRPAGFGARSMDDSMPKY
                     INTAATPVFDKRSTLYGFHMAREAIRDSDLGVIVEGYMDVIAAHEYGYKNVVASMGTA
                     LTVEQVRQLKNLASTFVLALDQDMAGQEATLRSLESAWQIFGDSSKRSSDPLFAGNPT
                     KINVVSLPEGKDPDEFIRSEGSDWELVVSDALPLMEYLIPVVSGRFDLDAPGGKGRVV
                     ESLAPILRLLDPFDREKYIGMLAEQLSASVGTVETALKQFRRGFNQRDRNAEGKGYKE
                     FEDQKSGDKNYKLDEYTLSLIINRPELKEVAVELNPECFARTEDREIYVRWLNLDPSE
                     SLIKLLDPVLEERFSSIQSYTMIPSSNTDTLIDFEACIKRLERRRLQLYRSDLIQSQN
                     TDSPPSSDLQNELSGLDKKILETYKGDKEGN"
     gene            8220..9770
                     /gene="rpoD"
                     /locus_tag="QF838_05295"
     CDS             8220..9770
                     /gene="rpoD"
                     /locus_tag="QF838_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HJO60131.1"
                     /translation="MRGSRSKEDGYRADEEEQEEQDELIDDDDDDDDDDEDSTVLELG
                     MDGRSTRREAEEDDDAPPSLEDLKAKAAREWEKEIGSTDIIDDPVRMYLREIGRVDLL
                     KAPQERDLARKFEGKRFVENKEEDLSPEQDTPPRAREIVADMLRTVVDSEDIIKAILT
                     SKEVAYDGTLPDLMANPEVREAIDGIFQDELLEQISAILSELRDEDPPETDQLKEMIK
                     QASINTRLLPDDIFKAIDGRPTMDQLKEIVADDSIVDKMQPYELLFRQHMERIKQRGD
                     DAQQHLSEANLRLVVSVAKKYIGRGMSLLDLIQEGNIGLIRAVEKFDYRKGYKFSTYA
                     TWWIRQAITRAIADQARTIRIPVHMVETINKLLRVTRRLVQEYGREPTSEEIGREMEI
                     APDKVREILKISQEPVSLETPIGEEEDSHLGDFIPDTAALAPVEAASYQLLREQVIDV
                     LDTLNDREARVLELRFGLEDGRSRTLEEVGKDFGVTRERIRQIEAKALRKLRHPNRSK
                     KLRDFLEN"
     gene            9771..10901
                     /locus_tag="QF838_05300"
     CDS             9771..10901
                     /locus_tag="QF838_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HJO60132.1"
                     /translation="MSLGTTKTNTGTQPEVVPNSDGTPTVAVMKTDPATVADDVGSAM
                     RLAGYNLQLAPDVATLLKINISWQHYYPACSTSPWQLDGVIKTLKEDGFDDLIPTHNG
                     TVVVDAREGEVNNKHDVVARRHGLESVHLEDLEWVPLPFSGDFLVLDQIYPDGIKIPK
                     FLIDKNVIHLPTMKTHVFTTITGAMKNAFGGLLNFQRHWTHSVIHETLVDLLRIQKKI
                     HPGIFAVMDGTFAGSGPGPRAMQWHEKNVLLAGADQVAVDAVAAKMMGFDPMSIDFIR
                     IADEQGLGCGQISKINIVGEDISDMNWHFQSGNTLASKGQKMIYWGNLKPLENMLLRS
                     KLTPLAYLASNIYHNEYWLRFKGKKRINEAMKTQWGRLFSEY"
     gene            complement(10929..11588)
                     /locus_tag="QF838_05305"
     CDS             complement(10929..11588)
                     /locus_tag="QF838_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HJO60133.1"
                     /translation="MDNLNLSVNKREVTGKKVSDLRSNGLTPIHMYGPEIESSPLQCD
                     SKILDRVITDAGTNIPVTVNVDGGEQDNLCFIREVQYHPVTNKVLHVDFMKVQVGKPV
                     RAQVPISVIGTSPAVRTMGGTLLQPLLTLTVEALPLEIPEAIILQAELLVDFETNFYV
                     SDIEVDEEVNVINEASEMVASVVAPRVDRIESEGEEGEETETEVVGEEAEESTGDDSS
                     G"
     gene            complement(11702..12847)
                     /locus_tag="QF838_05310"
     CDS             complement(11702..12847)
                     /locus_tag="QF838_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJO60134.1"
                     /translation="MTSENLGNIYKELGATPIINAIGSVTMLGGSTPIPEVREAMALA
                     GDSYIPLMELEEKAGSAIAEMIGVPAAYITSGAGSALTLAAAAVMAGENDESIEQLPD
                     TTGMKNQILIQKRQRYWYDRCLEASGAKLVEFGSDEGTTPQDLEKAINENTAAVHFYM
                     VEQEHDPNALTLEETIRIAHQNELPVLVDAAGQIYPLDLFGKYVKMGADFQCIAAKYM
                     GASQSTGLALGTEEMIRKISKQSFVGYEGRRVRGIGRPHKVDRQEMVGVVAAVKNWMT
                     INHEERLIEIETRTQRVINILSDTPAVTSKMINNIIGHQPFGVELKIDEEEAGMTLQE
                     IVDILKEGDPPIWTRVKEGDDYIAIHMFGLKVGEEELVAERIRELLS"
     gene            complement(12884..>13422)
                     /locus_tag="QF838_05315"
     CDS             complement(12884..>13422)
                     /locus_tag="QF838_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014090.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HJO60135.1"
                     /translation="VAGSLSMATGEYISVRSQKDIYEHQIDIARAKLEGCPESEEEEL
                     VLIYRAKGIEESQAKIIASALMADPNRALDTMVREELGLNPQDLGSPWLAAISSLLAF
                     ALGALIPIIPILFSSGNISIVFSAIFSSTALFIVGGIVSVASGRNTFLGATRMLLAGT
                     LAATFTYGVGYLLGISIL"
BASE COUNT         3871 a         2622 c         3052 g         3877 t
ORIGIN      
        1 atcttgatga tgttgaacac atccgcccat ctggattgat tattatgaac cttaagggtg
       61 atgatgagtt agtatctgtc aaattggcta agacaaatga cgacataatt ttcatatcag
      121 agcagggcat gggaataagg ttttcggtga atgatcttcc aatacgtcgt cgtactgcgg
      181 ggggtgtcaa aggaatgtca ttaagaacag gtgatagggt ggtttcaatg gatgtgggta
      241 acgatgacag taaattgctc gtcgtcagca aattaggacg aggtaaagtg aaccctctta
      301 gtgaatatag aaggcaaggc cgtggtggat taggattacg agccttcaaa ctcactaaaa
      361 acacagggtt aatagcagat gcccaaatag tggatgaaac caatgaggtg tacctagttt
      421 ctgagaatgc ccaggttatg aggactgatt tgagtgaaat aaggagtctg actggaagaa
      481 tcactcaagg tgtaacgata tttaagccta gagcaggtga ttcagtttct tcaatcgcat
      541 gcgtcggtga ctttgaactt gatgaggatg aggatccaaa acagactgac gcaaaggcat
      601 cgaaaaacgg taaaaattcc aaaaataaaa aatagggaat gcagtcagat acccatgtga
      661 tggtcgtagc ggggcgatgg gaatttcttt gtatcggcag cattcaactg ccttagttac
      721 gagccaaacc tgaaagatgg caataattag aaaattgcta ttgggtttgc aggtgaacct
      781 gttccgcctt gaacataaag tggattcaga cttagcataa attcgtacct tccttcttcg
      841 gcacaaacgt ttgaaagtgg ctcaagatac gcgttgtcga caagtgctac cccgtaggca
      901 aatatcacgc cgtggacggt ccaagggatt tcgtattcat tgggtgaggc gtccatcata
      961 tcccaaccca agatagatat attattgctt tttacgaacg ggagacatga agcatggagg
     1021 cctggtctac cattttgggt accccatcta ccattattgt cagcagcata tttgtcgcgg
     1081 ccactgtata cgaagacggc atcaccggat tttatttcca cattctgttc tgtggcgata
     1141 tcttgcaatt cccatccatg aaccggttcg cttagggtta catatggctt gtttctatgt
     1201 ttaggtacgt ctagtaatat gccgcgggtt aagattccat ctttccattc ttcaacggta
     1261 ccaaaattcg caccgccaaa tttaatgaca tcatcaggcg ttttaccttc ccacattcca
     1321 tttttatccc agacgtggca tagagcatct atatgagtcg tagcagtacc atggtaaaaa
     1381 acaccgtagt aatccatagc ggccccaccc tcaaatggtc tttcctcttt gtgcatataa
     1441 tgctgggccg gcctgggatt atctcctgcg ggggttactg gtaagggcct acttaaagat
     1501 actgttcttc caattttagc tagtgacact gcctctaagc gtttctcgtc tgttatcaag
     1561 ttcattgccc cagcggaact tttatcgccc cacctgcccc agttagtatc ttccagataa
     1621 gaatcaactt ctgatttaga aggtatattt ctgcctgcca ttactgcgtg actcctttat
     1681 caatttacat gatgcaactg aaatatgatt agtccgctat ggttacacca gccatatcct
     1741 ccataaaatt agctatagca gagtgatcag agttgccctt gccatcgtta ataagggacc
     1801 caagtatttg ctgcaccatg gcggttacca ttaaaggaac tccgacatct tttcctgtta
     1861 gtatggcatt gtgtagatct ttctgatgga gaactatcct gaagccagga tcgaaatttc
     1921 cctctagcat cattggtcct tttgtattca ttacagtgct accagccaat ccccctttga
     1981 tagcttcaaa tactacttta ggatcaatcc cagatttttt cgctaataca agtgcttccc
     2041 ctaaagcagc gatatttcca gccactataa tttggtttgc taattttgta gtgtttcctg
     2101 cgccgtaccc tccacaaaga actatgctgc cggacattgc agttagcatg tcataattct
     2161 tttcaaaaac ctctttttcg cctcccacca taaccgcaag ggttccctca atagctcctg
     2221 tttcccctcc actcactggg gcatctaaga aatttacacc ggcagcgtcg caggctcctc
     2281 ctatcttttt tgaggatgca ggggcaatcg aactcatatc gatcacagta tcgccttttg
     2341 aagccccttc taatacaccc cctggcccga ggattgcagc ttctgattgg ggagagtccg
     2401 ggaccattgt tataattatg ggggttttag atgctacttc tgctgcggaa gacgctgggg
     2461 tagcaccttc agtggccatt tcttcaactg cttctgcgac tatgtcataa acagtcacag
     2521 cgtagtctgc ttttattaag ttcctcacca tgggccttcc catgattcct aatccaacaa
     2581 aacctatttc acctcgtgcc attttgacga ctccttcttc tagttgattc ccgtaaaacc
     2641 ggagaccaat acatgtcttt aatttaactg cccttttcct ttcacggaca ccccaattat
     2701 agacttagat gccactcggt caacattagg ttggataagg aataatgttc ccagtatatg
     2761 cccgtgttac ttaacttcaa ttaatggttg tctgttatta ttccgatgta attttattca
     2821 ttgattgatt aataaaaatg ggaggtagaa ggtgtctgaa ttaacccaaa atgaaattga
     2881 ttctgttgct actatgcaaa cttgcgccgt ttccaatgct attgaaagtt taaatatcag
     2941 atcgagaacc gaaggattca tgactcctga tataaaaagt atgtttccag atatgggatc
     3001 tatggctggg tttgcagtta ccgcggttat caaagcctct actccacctt cagacaacat
     3061 gaattattct cgagtggaat gggttgatga gatactaaag attccaggcc caagagttat
     3121 tgtaatgaaa gatttggatc atccatttgt aatcggatct ttctggggtg aggttcaaag
     3181 tgccattcac agttctctgg attgtgtcgg agttgttact gatggagggg ttcgggatct
     3241 ggatgaaatg caagaatggg ggtttaatgc cttcgcaagt acagctttgg tttctagggc
     3301 caatgttcac ctggtagaag caaacattcc agtcactgtc ggtggtgtca cagtgaatcc
     3361 gggagattta cttcttggag ataaacatgg ggtgattagt attccgaaag agattgctaa
     3421 ggatttgccc gatgctattg ctaaagtgga ggcaggtgaa agagagatta tagacgtctg
     3481 caaagcccaa aatttttctc cagagaaact caaagatata cttcgccaaa gatacggcgg
     3541 ttaattaatt taaggaaaac agatggcttc accgaaagta gtgtttttga ctggactaaa
     3601 agaggaatta atagatgagg ttatttccta tagtcccacc gattaccaaa ttgtagtact
     3661 agataaatcc tccacagaac aacaacaaat agacgaggtt cgtgatgcgg atttcttatt
     3721 gtgttatgga caagatcctt caaacgcagt catcagatcg ttagaaaaat gtcgccttgt
     3781 acagctacta gccgcaggat acgatcgtat gaatttagaa cttctttcag agcttgaaat
     3841 accttgtgcc aataatggag gtgccaactc atgggccgtt gctgaccagg cgctgctttt
     3901 aatgctcgcg atatataaac agcttttggc aagtgacaat gcgaccaagg gagggcgttg
     3961 gtctgaacca ataaccggtc aaaatacatt tgagatggct gataaaaaag tgggaatttt
     4021 aggtatcggt aatattggaa ggcaggtagc taaacgtgtt caagggtttg atgctgaggt
     4081 acagtacttt gacttatacc cccttgacga gaaagtggca gaggaattga atgtcaagta
     4141 tgtgtcgtta gaagaacttt tcaggacttc tgacataatc acatgccaca cccctttgac
     4201 aaaagaaaca aaacatatag ttaactcgaa cacgctcgct atgatgaaac ctactgctat
     4261 tttgataaac acttcccggg gaccggtagt ggacgagacc gctttgatta gtgccttaga
     4321 agaggggctc atcgctgcag cagggttgga cgtttttgaa aaggaacctg tggatccgga
     4381 caatccctta ttaggaatgg acaatgttgt agctactccg catatggcag ggaccacttg
     4441 ggatacttgg gctagaaggg ccaattttgg ttttgaaaac atggaaaggg taaggcaggg
     4501 agaggcccca caggcagtgg ttagaaattt cgattccagc tgaaccaact cggctagagg
     4561 ataaaatgat gggacagggt gtcagacttc gactcgaaaa gaacgaggaa aatttaaatc
     4621 tttatgcctg ccggtcagcc tattctcgtg gtaggaggca gattgaagac cctagcccta
     4681 taaggacaga atttcaaagg gaccgtgatc ggatacttca ttccaaagca tttcgaagaa
     4741 tgaagcacaa gacacaagtt ttcatatcgc ctgtaggaga tcattttgtt actagactta
     4801 ctcatacact ggaggtttct cagattgctc gaactatagc aagggctttg aatttaaatg
     4861 aagacctaac tgaggcaata ggtttgggtc atgatatggg tcacactccc tttggccaca
     4921 taggtgaaga tgaattaggt aaattaagtc ctttaggatt taggcataat gtccagagtg
     4981 tcaggcttgt ggaggaatta gaaaagaatg ggaagggatt aaatcttaca gaggaggtaa
     5041 gggaaggaat gcttcatcat tcgaaaccaa agggggattt tatgtcctca aacttggttg
     5101 aaggtattgg ccttgaggcc caagttataa ggatttctga tgctatcgca tacctcaacc
     5161 atgatttagg ggatgctttt agggcgggtg ttttaaagga agcagacttg cctcatgaga
     5221 ccaaggagtt tttagggaga agacactcag agcgggtgga tcgtatggtt atggacatag
     5281 tttgtacttc cagtaggtta ttggaagata cctcattaac gtcccgccct acaatttcca
     5341 tgagtatatc aatgcgagat gctataaatg ttcttcgtga ctttatgttt gaaagagtat
     5401 atatacctgt agattcgggc ccagaaggta aagctgcgag attaattatt aatgttctat
     5461 acgaggcatt agatagtaat cataatctaa taccaaatga atttttggaa ataggggaga
     5521 ccgttgatca ttcgattgca gattatattt ctgggatgac tgatggcttt gcaattagga
     5581 ccgctgaatc cctatcccct ggtgccggac aggttttcgc cagttaaatt aataactatt
     5641 gttataatcc cgaaatgact gaatcaacta atcccgttga tagattaaaa accaggttgg
     5701 tttttaagaa catcgatcat atacaagaac atttagaggc catgcaaagg gacccgcatg
     5761 gtttggagta tcggccttgg aaacttgagg tggacaatat atggaaaaaa atatttgcgg
     5821 acatcaacga aatgtcagag gagtcgcaga agacggtttt ggatgcaatg agggatgtat
     5881 gggttagtta cattactcat tacggtgccg ttgaaggcta agccagtaag gagtgtatag
     5941 gaatgtcttt gagttttaac cccaatatgg agcaagcaag aaggcgttct gggttagcgc
     6001 atagggtctt ggtcaaactg aaaaccatgg gtttatctga cgatcatgat gattcactcg
     6061 ctatattgtg taccgacata ggagatttgt ggagttctca gttggtattt cttgaaatac
     6121 ttaatagatt cttggaagaa tcggataatt gggattccat tggagacgat tttgctgata
     6181 tgttgtccga cgtagaacac atctcgtggc atattgatag cttaaaaaag ccgttggaaa
     6241 tactcgccca atattcatat tcccaatcaa aaaataccga ataatacttg ttatgagtga
     6301 aatagacgaa ataaaatcaa aattagatgt tgttgacatt gtgtctgaga aggttcgttt
     6361 acagcgtgca ggcaggaatt acaaagctaa ctgccctttt catagtgaga aaactccgtc
     6421 ttttatagtt gaccctggaa gacaatcttg gagatgtttt ggatcttgtg gtgttggtgg
     6481 ggacgttttt agttttgtta tgaaaactga aaatatggaa tttcccgacg ctttaaggat
     6541 gcttgctcaa cgcaccggcg ttgaattacg tggctataaa aagaacgaat caagggactc
     6601 ctattttgag atcaataaga tagctttgga tttttataaa gacgcactct tgacggatga
     6661 agctcattcc gcgcgtatct acctcacctc aagaggcgtt gatgaaggtt cgattgaaga
     6721 ttttgggctt ggctatagcc cacgaggccg agattcacta aagtctcacc tattgttcca
     6781 tgatgtggat ctggaaactg ccgtcgaatg tggtttatta aataaatttg aggatggaac
     6841 cacgagagac tttttctggg gacggttgat gtttccaata ttcgataggt tgggaagacc
     6901 agctggattt ggggctcgct ctatggacga ctcaatgccg aagtacataa acaccgcagc
     6961 taccccggta tttgataaaa ggagcacttt atatgggttc catatggcta gagaggccat
     7021 tagagatagt gatctgggtg taattgttga aggttacatg gatgtaattg cagctcacga
     7081 atatggttat aaaaatgtgg tagcaagtat gggaacagct ttgactgtag agcaagtcag
     7141 acagcttaaa aacttggctt ctacctttgt gttggccctg gaccaagata tggccggtca
     7201 agaagccact ttacgaagtt tggaatccgc ctggcaaatt tttggagatt ctagcaaaag
     7261 gtcaagcgac cctttgtttg cgggaaaccc cacaaaaatt aatgtggtgt cattgccaga
     7321 agggaaagac ccagacgaat ttattcgttc agaagggtct gattgggaac tggtcgtaag
     7381 tgacgcttta ccacttatgg aatatctaat tccggtggtg tcagggcgat tcgatttaga
     7441 tgctccaggc gggaagggtc gggtagtgga atctcttgct ccgattctta gattattaga
     7501 tccttttgat cgtgaaaaat atattggtat gctagcggaa caattatctg catcagtggg
     7561 tacggtagaa actgccttaa aacagtttcg aagaggattc aatcaaaggg atcgcaatgc
     7621 agagggaaaa ggatataaag aatttgaaga tcaaaagtca ggagataaaa attacaaact
     7681 tgatgagtat acgttgtcac taataatcaa ccgaccagaa ttgaaagagg tcgcggttga
     7741 actaaacccg gaatgttttg ccagaaccga ggatagggaa atttatgtta ggtggttaaa
     7801 ccttgaccca agtgaatctt taattaaatt attagatccc gttcttgagg agagattctc
     7861 cagtatacaa agttacacta tgattccctc aagtaatacc gatacactga tagattttga
     7921 ggcgtgcatc aagagattgg aaagaaggag attgcagctt tatagatcag acctaattca
     7981 atcacaaaat accgattccc ctccttcaag tgaccttcaa aatgagctaa gtggcttgga
     8041 taaaaaaata ttggagactt ataaagggga taaagagggg aattaagtgg tatctttgtt
     8101 aaaatgtatt gcgatagttt gaaagttttt aacctgcacc aatatttgga aaggagaagg
     8161 gaatgactac tgatggaatt cagcatgtag gaggagaggc agatcttata gatggtgctt
     8221 tgagaggaag tcgatcaaaa gaggatggat atagagctga tgaggaagag caagaagaac
     8281 aggatgaatt aatagatgat gatgacgatg atgacgatga tgatgaagat tcaacagttc
     8341 ttgagttggg catggatgga cgttctacga ggcgagaggc ggaagaagat gatgacgccc
     8401 ctccatctct tgaagatttg aaagctaaag ctgctcgcga atgggaaaaa gaaattggaa
     8461 gtacagatat aattgatgat cctgttcgca tgtacctcag agaaataggc cgggttgatc
     8521 tcttaaaagc tccccaggaa agggatttgg ctcgaaaatt tgaaggcaaa cgatttgtag
     8581 aaaacaaaga agaagatttg tcacctgaac aggacacccc accaagagca agagaaattg
     8641 ttgcagatat gcttcggact gttgtggata gcgaagatat cataaaagca attcttactt
     8701 ctaaagaggt tgcatatgat ggaacattgc ctgatttaat ggctaaccca gaggttagag
     8761 aagccatcga cggcattttc caggatgagt tactcgaaca aatttctgcg attctgtcgg
     8821 aattgagaga tgaggatccg cctgagacgg atcaattgaa ggagatgatt aagcaggctt
     8881 cgataaacac tagactactt cctgatgaca tatttaaagc aattgatggt agacctacaa
     8941 tggatcaatt gaaggaaata gtcgcggacg attccattgt ggataagatg cagccatatg
     9001 aacttctctt taggcaacat atggaacgta taaaacaaag aggtgacgat gcccaacagc
     9061 acctttcgga agccaatttg cgtttagtag taagtgtggc aaaaaaatat atcggtcgag
     9121 ggatgtccct tcttgatctt atacaggaag gtaatatagg actgattaga gctgtagaga
     9181 aattcgatta cagaaaaggt tacaaattta gcacatatgc tacttggtgg atcaggcaag
     9241 ccatcacgcg tgcgattgct gatcaagcta gaactatacg tattcctgta catatggttg
     9301 agactatcaa taaattgctc agggttacca gaagattagt ccaagagtat gggagagaac
     9361 cgactagtga agagattggt cgtgaaatgg agattgctcc agataaggtt agagagattt
     9421 taaaaatatc tcaggaacct gtttcgttgg aaacaccaat tggggaggaa gaggatagcc
     9481 atttgggtga ttttattcct gacacggcag cattagcccc ggttgaggcg gcctcctatc
     9541 aacttcttcg agagcaggtt attgatgtgt tggatactct taatgacaga gaagcaagag
     9601 ttctggagct caggtttgga ttggaagatg gtagaagtag aactcttgaa gaggttggca
     9661 aggattttgg tgtaactagg gagagaattc ggcagataga agcgaaagca cttcgtaagc
     9721 tacgccatcc taacagatca aaaaaattga gagacttcct agagaactaa atgtcgctgg
     9781 ggacgactaa gactaatact ggtacccaac ctgaagtggt acccaactct gatgggactc
     9841 cgactgttgc ggtgatgaag acagatccag cgactgtggc tgatgatgtt gggtctgcta
     9901 tgcgtcttgc agggtacaat ttacagttgg cccccgatgt tgccaccctt ttaaagatca
     9961 acatatcctg gcagcattat taccccgctt gttccacgtc cccctggcaa ttagatgggg
    10021 ttattaaaac tctaaaggaa gatggatttg atgatttaat tcctactcac aatggaacag
    10081 ttgtagtgga tgctcgggaa ggtgaagtta ataacaaaca tgatgtggtt gcgaggcggc
    10141 acgggctgga gagtgtacat cttgaggacc tcgagtgggt gccgctacct ttttcgggcg
    10201 acttccttgt tttggatcaa atttatcccg atggtataaa aatacctaaa tttctgatcg
    10261 ataagaatgt gattcatctt ccaactatga agactcatgt ttttactact attacgggag
    10321 cgatgaagaa tgcatttgga ggattactga attttcaaag acattggacg cactctgtaa
    10381 tacacgaaac tctagttgat ttattgagga tacaaaaaaa aattcaccct ggtatttttg
    10441 ctgtgatgga cggcactttt gccggttccg gaccagggcc ccgagctatg caatggcatg
    10501 agaaaaatgt tttattagca ggggctgatc aagtagctgt ggatgcggta gctgcgaaaa
    10561 tgatgggatt tgaccctatg tccattgatt ttatcaggat agccgatgaa caaggattag
    10621 gctgtggcca aatatctaaa ataaatattg ttggagaaga tatttccgat atgaattggc
    10681 attttcaaag tggtaacacc ttagccagta agggtcaaaa aatgatttat tgggggaatt
    10741 tgaagccact tgaaaacatg ttgttgagat ccaaattgac ccctctagct tatctcgctt
    10801 ctaatatata tcacaatgag tattggcttc gattcaaggg aaagaagcgt ataaacgaag
    10861 ccatgaaaac ccagtggggg cgtctttttt cagaatatta gcccagttaa aacaactgag
    10921 catattatct agcctgaact gtcatctcca gtagattctt ccgcttcttc accgacgacc
    10981 tcggtttcag tctcctcccc ttcttcgcct tcagactcaa tcctgtccac tcttggagct
    11041 acaacgctag ccaccatttc agacgcttca ttgatcacat ttacttcttc atccacttct
    11101 atgtcgctaa cataaaaatt tgtctcaaag tcgaccaata attctgcctg taatatgatc
    11161 gcctccggta tttctagagg taatgcctcc accgtcagtg tcagtaatgg ttggagtagg
    11221 gtacctccca tcgttcgcac ggctggggag gtgcctatga cagatatagg tacttgagca
    11281 cgtactggtt tgccaacttg tactttcatg aaatccacat gtaaaacttt attggtcacc
    11341 ggatgatact gtacctctct tatgaagcag agattatctt gttctcctcc gtccacattg
    11401 acagtaaccg gtatgttcgt gcctgcatca gttatgacac gatctagaat tttcgaatcg
    11461 cattgaagag gactggattc tatttcagga ccatacatat gaattggggt taatccattg
    11521 cttctgaggt cagatacctt ttttccagta acttctcttt tattcacact taaattcaga
    11581 ttatccacct ggaagagctc ctgtattaac gtaactgcag caaaaagctg aatggtttaa
    11641 attatatgaa accgataaat tttagcatag gaaaaagttc tcatcatcct tggcttgttg
    11701 tttaactaag taactctctg attctttctg caaccagctc ttcttcacca actttaagcc
    11761 caaacatgtg aattgctata tagtcgtcac cttctttcac acgagtccat atcggtgggt
    11821 ccccttcctt taagatatct actatctcct ggagggtcat accggcctct tcctcatcta
    11881 tttttaattc gacgccgaaa ggttggtgtc cgatgatgtt atttatcatc ttagaggtaa
    11941 cggcgggtgt atctgaaaga atatttatga ccctttgggt tctggtttca atttcgatga
    12001 gtctttcttc atgatttatg gtcatccagt tctttacggc agcaacaacc cctaccattt
    12061 cctgcctatc taccttatgg ggtctgccta tccctcttac ccgccgtcct tcgtatccca
    12121 caaatgattg cttagagatt ttgcgaatca tttcttctgt acctaatgcc agaccggttg
    12181 attgtgaagc gcccatatat ttagctgcta tacattgaaa atctgcaccc attttaacgt
    12241 atttaccaaa taaatctaaa ggatatattt gaccagctgc atcgactaaa acgggaagtt
    12301 cattttggtg ggctattcgt atagtttctt caagtgtcaa ggcatttggg tcatgctcct
    12361 gctctaccat gtaaaaatgt acggctgctg tgttttcgtt tatagccttt tccaaatctt
    12421 gaggggtagt accttcatcc gaaccaaatt ccaccaattt tgcaccagag gcttctaaac
    12481 atctgtcata ccaatagcgc tgcctttttt ggataagaat ctggtttttc attccagttg
    12541 tatccggtaa ttgttctatt gattcgtcat tttcaccagc catgactgcc gccgctgcta
    12601 aagtcaaagc tgaacctgcc cctgaggtta tgtatgcagc cgggacacct atcatttcag
    12661 ctattgcaga tccggctttt tcttccaatt ccattagcgg gatataggag tccccagcaa
    12721 gtgccattgc ttccctaact tcaggaatcg gtgttgatcc acccagcatt gttacactac
    12781 caatggcatt tataattgga gttgcaccaa gttctttata tatatttcct agattttctg
    12841 atgtcattgt agtcctccgg atatgagatt tgattttatt gacctataaa attgaaattc
    12901 ctaataaata accaacaccg taggtgaagg tggcggctaa ggtacctgct aaaagcatcc
    12961 ttgttgcacc taagaaggta tttcttccac tcgctactga aacaattcct ccgacaatga
    13021 atagggccgt tgagctaaat attgcactaa agacgataga tatattacct gaagaaaaaa
    13081 gaattggaat tattggtatc agtgctccca aagcgaaagc taataaacta ctaatcgcag
    13141 ctaaccatgg agatcctaaa tcctgtggat taagtcccaa ttcctctctc accatggtgt
    13201 ctagtgcacg atttggatca gccatcagtg ctgacgctat tattttggct tggctttctt
    13261 caatgccctt tgctctgtat atcagtacta attcctcctc ttctgactca gggcaccctt
    13321 ccagttttgc ccttgcaata tctatttgat gttcatatat atctttttgt gatcgaacag
    13381 aaatatattc accagtagcc attgataaac taccagcaac aa
//
LOCUS       DASCNS010000148         6965 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001177259, whole genome shotgun sequence.
ACCESSION   DASCNS010000148 DASCNS010000000
VERSION     DASCNS010000148.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001177259"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /gene="dnaE"
                     /locus_tag="QF838_05320"
     CDS             complement(<1..1707)
                     /gene="dnaE"
                     /locus_tag="QF838_05320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HJO60136.1"
                     /translation="MFTHLHVHTEYSMLDGLARLDPLVDKAAALGMKSLAITDHGGLY
                     GAVDFYRKAKRSGLKPIIGCEMYVATGSRFDRNPSDKIHHLTVLAKDATGYKNLVKLV
                     TSSHLEGYYKRPRVDRELLEAHSSGLAVLSGCPSGEVPWLIAQGRYDEAKKVSGWYKE
                     VFGAYFLELMQHGGVPELPEINKGLLRLNTETQIPLVATNDSHYVEKQNAIDHEVLLC
                     IQTNTNIQDERRMRFEEDSYHLKTHDEMSLLFPDSPSAIANTEMVAEMCELELDFSQA
                     RLPEFPVPPGMTSDQYLAEICWKGYEEKVRHKTQEYKARLEYELKVIEQTSFPDYFLV
                     VWDIAKFVRENDIFFTVRGSAAASLVLYCLGVTDVDPMPFKLVFERFLNIERKEMPDI
                     DMDFQDDRRQEVINYCSARYGREHVAHIITFGTFGARQSVRDAGRALGMSLESVDRVA
                     KMIPERLNIDLESSLLESQDLGNVYQTSSDVKKLMDTARQLEGVTRHKSLHAAGVVIS
                     KEPLNDVVPLEFTSRGDDDGAVMTQYSMEPVAALGLLKMDFLGLVNLTVLDETLKLIK
                     LKH"
     gene            complement(1923..3533)
                     /gene="groL"
                     /locus_tag="QF838_05325"
     CDS             complement(1923..3533)
                     /gene="groL"
                     /locus_tag="QF838_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871329.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HJO60137.1"
                     /translation="MAKQLTFSEEARAAMKRGIDTMADTVGVTLGPRGRNVVLDKKFG
                     PPQVCSDGVTIAKEIELEDAFENMGAQLLKEAASKTNDVAGDGTTTATVLAQAIIHEG
                     FKNIAAGANPMAIKRGIDKAVETLRSAVQGMSTPVEGRVQIGQVASLSAHDDDMGNLI
                     ADVMEKVGKDGVITVEESKGLDYEQEFVEGMQIDRGYISPYFITDQDRMESSIEDPYI
                     LITDKKVSAVSDLLPALEKVLQIGKNVIIIAEDIEGEALATLVVNKLRGTLNVLAVKA
                     PGFGDRRKAMLEDMAILTGGIVISEEVGRKLDSVTVEDLGRARRVVASKEDSTFVEGH
                     GDESQIQGRINQIRAQIEETTSDFDREKLQERLAKLSGGVAIIKVGAATEVELKEKKN
                     RVEDALSATRAAVEEGIVPGGGLALIRARESLDAVELNGDEQTGVNILKAALVKPLML
                     ISENTGVSGEVILAETLKGDGDYGYDAETGVYGNLLEQGIMDPAKVTRAAIENASSVA
                     AMVLTTESLISDIPEAAPPAPAMPPMDY"
     gene            complement(3590..3883)
                     /locus_tag="QF838_05330"
     CDS             complement(3590..3883)
                     /locus_tag="QF838_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HJO60138.1"
                     /translation="MTTSFKPLGNRVVVEPLEGEEQMSSGGIYIPDTAKEKPQEGTIV
                     AVGPGRLTDDGARVPMELEVGDSVVYSKYAGTEYKEGDIEYLVLREDDILFKK"
     gene            complement(4097..4573)
                     /locus_tag="QF838_05335"
     CDS             complement(4097..4573)
                     /locus_tag="QF838_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013756.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HJO60139.1"
                     /translation="MSNSDLFRKAWGNFATGVSLITTVEENGSVHGMTANGIASISLE
                     PMLAMVCVGHSAKTYPILESTGKFSINILGENQKSIGEYYAKSDTPDGVTSPAIFKFT
                     KTGTPFLEGSLASMDCQVINAHKEGDHTVFIGEVSEIEVSEGSPLLFYTGKWMTLT"
     gene            4773..5018
                     /locus_tag="QF838_05340"
     CDS             4773..5018
                     /locus_tag="QF838_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013224.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HJO60140.1"
                     /translation="MSTTAYILIETAAGKAGNVSNALMALDEVVTVDAVIGPYDIIAV
                     TNTEGQNTVGDLVISKVHVIPGIVRTVTCLAVTPSQK"
     gene            5078..6250
                     /locus_tag="QF838_05345"
     CDS             5078..6250
                     /locus_tag="QF838_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60141.1"
                     /translation="MDFEFSNQELSFRNELSAWLKDELANRPQDGGDDADAEWAFGLE
                     MRKKLADKGWLTMAWPEEYGGQGVSHMMQVVFAEEMSYNRAPGRDVFGTRMMAPTLMI
                     HGTEEQKRQFLPPVSKGEVQWCQGYSEPESGSDLASLQTRAVEDGDDFIINGTKIWTS
                     SAHRADHIMVLTRTDPDAPKHRGISFLLCDMNTPGLTVNPIINMAGDHGFNMVTFDNV
                     RVPKKNLVGEQNRGWYVGATLLDFERSGVDYSAGGRRVLEELIAYAKDNDQNGSLIAD
                     NPIMRNRLADLAIEVDVSRLISYNIAWMQGEGLVPNKESSMGKVVGTELQQHLSETGM
                     QMLGLHGQLEPGSKYAPMQGRIEHMHLTNVSETIQAGTSEIQRNIIATRGLGLPRG"
     gene            6297..>6965
                     /locus_tag="QF838_05350"
     CDS             6297..>6965
                     /locus_tag="QF838_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014790.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60142.1"
                     /translation="MDFKFENIDEDFRSEIKDFVEKELPWNWREQDLDPEDEKDSVLV
                     REFKRKLGDQGWLTMAWPKEYGGQAASHMRQVVFNEEMAYRGISAGDAGIRMVGPILM
                     LYGTDEQKEQFLPKIASAEINWSQGYSEPDSGSDLASLQTRAVEDGDDFVVSGSKIWN
                     SAHADSDWMFMLVRTEPDAPKHRGISFVLTELDGQEGVTIEKIPMMWDAYRGLVTFDN
                     VRVPK"
BASE COUNT         2001 a         1595 c         1404 g         1965 t
ORIGIN      
        1 gtgtttaagc tttataagtt tgagagtctc atccaatacc gttaaattta caaggcctaa
       61 aaaatccatt tttagaaggc ccaaagctgc cactggttcc atggaatatt gagtcataac
      121 tgcaccgtca tcgtcaccgc gtgaagtgaa ttccaaaggt acaacatcat tcaagggttc
      181 ttttgagata actaccccag cggcatgtag gcttttatgc cgagtgactc cttctagttg
      241 tctagcagtg tccatgagtt ttttgacatc tgaacttgtc tggtaaacat tgccaaggtc
      301 ttggctttca agtaaagaag attccaaatc tatattgagc ctttccggaa tcatctttgc
      361 aactctgtca acactttcta aagacatacc caaagcccgg cctgcatctc taactgactg
      421 gcgagccccg aaggtgccaa acgtaataat atgggctacg tgttccctac catagcgagc
      481 cgaacagtaa ttaatcactt cctggcgacg atcatcctga aaatccatat caatatctgg
      541 catttctttc cgctcgatat tcaaaaatct ttcaaagact aatttgaatg gcatcggatc
      601 aacatcagtc acacctaaac agtaaaggac aagacttgca gctgcactac ctctaacggt
      661 aaaaaatatg tcattctctc taacaaactt agcgatgtcc cataccacca aaaaataatc
      721 aggaaagctt gtctgttcta ttactttcaa ttcatattcg aggcgggctt tatattcttg
      781 agttttatgt cggacttttt cttcatatcc tttccaacat atttcggcca aatattgatc
      841 cgaggtcatg ccagggggca cgggaaattc aggcaacctt gcttgagaga aatccaattc
      901 caattcacac atttccgcaa ccatttcagt atttgcaatg gcagaaggag aatctggaaa
      961 aagtaaactc atttcatcat gtgtttttag atgataagaa tcttcttcga atctcatcct
     1021 cctctcatct tgaatgttgg tattagtttg gatacatagc agaacctcat ggtcaatagc
     1081 attttgtttc tccacataat gagagtcatt tgtggcgacc aatggaattt gtgtttcagt
     1141 atttagtcgt aataaccctt tgtttatttc cggtagctca ggcaccccgc catgttgcat
     1201 caattccaaa aagtaagcac caaagacctc tttataccat ccagacacct tctttgcttc
     1261 atcataacgt ccttgcgcaa tcaaccaagg aacctcccca ctcggacatc ctgataatac
     1321 cgccagtccg gatgaatgag cttcaagaag ttctctatct accctagggc gcttgtaata
     1381 gccttctaaa tgagaggatg taaccaattt aactaagttt ttatacccgg tggcatcttt
     1441 ggcgagtact gtcagatgat gtattttatc ggatggatta cggtcaaaac ggcttcccgt
     1501 tgcaacatac atctcacagc caattattgg ttttagtccg cttcttttgg ccttccgata
     1561 aaaatcgact gcaccataaa ggcctccatg gtcggttatg gctaaacttt tcatccccag
     1621 ggctgcagcc ttatcaacta gaggatccaa tcttgccaat ccgtccaaca tgctgtattc
     1681 ggtatgcacg tgaagatgtg tgaacaagtt aactaccttt aaagcgattt gtagacccaa
     1741 ttactggaaa aggcactaat tagatcgtta actatgaacc tacgctacca gtatcatgtg
     1801 attatagtgc caactgtatc ttgatgcgca aggtgtggaa atagaaatcc aaggtcaatc
     1861 tctccaaaaa aaaaggcccc cagaacgatc tggagacctt ttcatcttct aagaattttt
     1921 tcttaataat ccatcggagg cattgctgga gcaggcggag ctgcttccgg aatatcactt
     1981 atcaaggatt ctgtagtcag aaccatcgct gcaacgctgg atgcgttttc aatagcagct
     2041 ctggtaactt ttgctgggtc cataatccct tgctctaata agtttccata cactccggtt
     2101 tctgcatcgt atccatagtc tccatcaccc ttcagggttt cggccaaaat aacctctccc
     2161 gagacgccag tattctcaga aatcaacata agaggtttga ctaaagccgc tttcaatata
     2221 ttgaccccag tctgttcatc tccattaagc tctacagcat ccaaagactc acgtgctcgg
     2281 atcaaagcca atccgccacc tggaacaata ccttcttcta ctgcagctct tgttgctgac
     2341 aaggcatctt caactcgatt cttcttttct ttcagctcaa cttctgtggc ggcgcccact
     2401 tttattatgg caacaccacc tgacaattta gcgagccttt cttgaagttt ttccctatca
     2461 aagtcagaag tagtttcctc tatctgagct ctaatttgat ttatccttcc ctgaatttga
     2521 ctttcatcac catgaccttc aacaaaggta gagtcttcct tactagcaac aactcgacgg
     2581 gcacgtccaa gatcctcgac tgtcactgaa tccaactttc ttccaacctc ttcgctaata
     2641 acaatccctc cagtcaagat agccatgtct tccagcatag ccttcctgcg gtcgccaaag
     2701 ccaggtgctt ttacagcaag tacgttcaac gtacctcgta atttattgac tactaaggtg
     2761 gctaatgcct caccttcaat atcctcagct atgataataa cgttcttacc tatttgcaga
     2821 actttttcca acgctggcag taaatcagat actgccgaaa ccttcttatc tgttatgagt
     2881 atgtagggat cctcaatgga tgattccatg cgatcttgat ccgttatgaa ataaggggat
     2941 atgtatccac gatcaatctg catcccttca acaaattcct gttcataatc aaggcctttt
     3001 gattcttcaa cggtgattac accatctttt cctaccttct ccatgacgtc agctataaga
     3061 tttcccatat catcgtcgtg agcagacaag gaggctacct gaccaatctg cactcttccc
     3121 tctactgggg tggacatgcc ttgaacagcg gacctcaacg tttcaacagc cttatctata
     3181 cctctcttga tagccattgg gttcgcccct gcagctatgt ttttaaaacc ctcatgaata
     3241 atagcctgtg ccaatactgt ggcagttgtg gttccatctc cggctacatc attggtttta
     3301 ctagcggctt ctttgagaag ttgtgcaccc atgttttcaa aggcgtcttc taattcaatt
     3361 tccttcgcaa tggtcacacc atcactacaa acctgtggcg gtccaaactt cttatccaag
     3421 acaacattcc gtccccttgg gcccaaagta accccaacag tatcggccat ggtatctata
     3481 ccacgtttca ttgcagctct ggcttcttca ctgaaagtca gctgttttgc cataactccc
     3541 tcctactttt ttcagttaca aatgttaatt gctttattca ttcgtcttat tattttttaa
     3601 atagaatgtc gtcttctcta agcactaaat attctatatc tccctcttta tactctgtac
     3661 cggcgtactt ggaataaaca acactatcgc caacttccaa ttccatcggc actctggccc
     3721 cgtcatctgt aagcctccca ggccctacgg caacaattgt tccttcctgt ggcttttctt
     3781 tagctgtatc gggtatgtat atccccccgg agctcatttg ctcctcgcct tccaagggtt
     3841 ccacaactac tcggttaccc agcggtttga aagaagtagt catgaaaata aacctcctag
     3901 tctacttact aaaacctgtt ttatttgaac accaacctgc tttttatctc cacgctgtgg
     3961 acagtccctg aaaaaaaaag cgcagttaat ggtaagtcag ctacaaaatt attgtcaaca
     4021 gcaatctact tccagtgtga tcggctagta aacaagtagc actatattca ttcaatatta
     4081 tcttccgtat acaggattaa gtgagggtca tccatttacc ggtgtagaac agaagtggag
     4141 acccttcaga cacttcaatt tcagaaacct ccccaataaa aaccgtatgg tctccttctt
     4201 tatgagcatt aatgacctga caatccatgg aagcaagtga tccttccaag aaaggagtgc
     4261 cggtcttggt gaacttaaat attgcgggac tagtaacccc atctggagta tctgacttgg
     4321 cataatattc gcctatcgat ttctgattct cgcccagtat attgatacta aatttaccag
     4381 ttgattccaa tatgggatag gttttcgccg aatggcctac gcataccatt gccaacatag
     4441 gttcaagcga aatcgaagcg atcccgtttg cagtcatgcc gtgaacagat ccattttcct
     4501 ctacagtggt tattagagaa acccctgttg caaaattacc ccacgccttc ctaaaaagat
     4561 cggaattaga caatggaaaa cctccagatc acccttattt ttgatgctca accaccttaa
     4621 acacagcgat tatataaaac cgaacaccaa tttcctataa aggtgggata ttgaaatcga
     4681 attcctctgt taattgacac cgattcttcc agcaaaatag aattcatgta ttaatataga
     4741 ttagaaatca ttcgaggtgt ttaattctcg gtatgtccac taccgcttat attttaatag
     4801 aaaccgctgc aggaaaggca ggaaacgtat cgaatgcttt aatggcacta gatgaggtgg
     4861 ttactgtgga tgctgtgatt ggtccttatg acatcatcgc tgtcacaaac acagaaggcc
     4921 agaacacagt aggggatttg gtgatatcaa aagttcatgt gatccccgga atagtgcgaa
     4981 ctgttacatg ccttgctgtg acccctagtc aaaagtgaac atacgcaacg tgcacattgg
     5041 atttaaacct atgaaaataa atttaaggac ttagaaaatg gactttgagt tttcaaatca
     5101 ggagctttca ttcagaaatg agttgtcggc atggttgaaa gatgagctag caaatagacc
     5161 tcaagacggt ggagatgatg cagatgccga atgggcattt gggcttgaaa tgcgtaagaa
     5221 acttgcagac aaaggatggc tcacaatggc gtggccagag gaatatggtg gacaaggtgt
     5281 ctcccatatg atgcaggtgg ttttcgcaga ggaaatgtcc tacaacagag cccctggaag
     5341 ggatgttttc ggaactcgaa tgatggcacc tactctgatg atacatggaa cagaggaaca
     5401 aaaaaggcag tttttaccgc cggtatcaaa aggggaagtt cagtggtgcc aaggctattc
     5461 tgaaccggaa tccggatctg atctagcctc tcttcaaact agggccgtag aagacggaga
     5521 tgatttcatc ataaacggta ccaagatatg gacatctagc gctcatcgtg ccgaccatat
     5581 catggtactt acaaggacgg atccagacgc gccaaagcat agaggcatta gtttcctttt
     5641 atgtgatatg aatacacctg gattaaccgt aaatccgata atcaatatgg caggtgacca
     5701 tggttttaac atggtcacct tcgacaacgt aagagtgccc aagaagaatc tcgtgggtga
     5761 acaaaacagg ggatggtacg ttggtgccac tttactggat tttgaaagat cgggcgtcga
     5821 ttattcagca ggaggaagaa gagttcttga agagttaata gcatatgcga aagataatga
     5881 tcaaaacggt tcgttgatag ctgataaccc catcatgcgt aaccggttgg cggatcttgc
     5941 tattgaagtc gatgttagcc ggttgatttc atacaacatt gcatggatgc aaggtgaagg
     6001 tcttgtaccc aacaaagagt catcaatggg aaaggtggtc ggcacagagt tgcaacagca
     6061 tttaagcgaa acaggaatgc aaatgctagg attacacggc caattggaac caggttcaaa
     6121 atacgctcct atgcagggca gaatagaaca tatgcattta accaatgttt ctgaaacaat
     6181 acaggcaggc acttcagaaa ttcagagaaa cataatagcc acccgaggat taggattacc
     6241 tcgaggctaa ataaagtagt agaatcagga accttatata ggaggagtaa ctaaaaatgg
     6301 attttaaatt tgaaaacatt gacgaagatt ttcgttcaga gatcaaagac tttgttgaga
     6361 aagagctccc ttggaactgg agagaacagg acctagaccc agaagacgag aaagattcgg
     6421 ttttagtcag ggagttcaag aggaaattgg gagatcaggg ctggcttaca atggcttggc
     6481 caaaagaata cggagggcaa gctgccagcc atatgcggca agttgtattc aatgaggaaa
     6541 tggcctatag gggtatatct gctggagatg ctggcataag aatggttgga ccaattctta
     6601 tgctctatgg aactgatgaa cagaaagaac agttcttacc aaaaattgcc agcgctgaga
     6661 tcaactggtc tcaaggttat tcagaacccg attctgggtc tgacctcgcc tcgctgcaaa
     6721 caagggctgt tgaggatgga gatgactttg ttgtcagtgg ttcaaagatc tggaattcag
     6781 cacacgcaga ttctgactgg atgttcatgc tggtccggac tgaacccgat gccccaaaac
     6841 atagaggaat tagttttgta ctgacagaac tggatggtca ggaaggggta accatagaga
     6901 aaattcccat gatgtgggat gcataccgtg ggctagttac atttgataat gtcagggttc
     6961 ctaag
//
LOCUS       DASCNS010000149         1729 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001182739, whole genome shotgun sequence.
ACCESSION   DASCNS010000149 DASCNS010000000
VERSION     DASCNS010000149.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001182739"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="QF838_05355"
     CDS             <1..386
                     /locus_tag="QF838_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014799.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HJO60143.1"
                     /translation="KPGTDVTLVAYSGMVRVAEEAAAKLEEDNISSEVIDLRCLSPLD
                     LDTVIESVKKTNRVAIVEETWKTGGFSGTIASDIQEAAFDELDGPVIRINGPDVPAPY
                     ARNIEQLMIPNSDWVVESVKYNFGL"
     gene            403..1692
                     /locus_tag="QF838_05360"
     CDS             403..1692
                     /locus_tag="QF838_05360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HJO60144.1"
                     /translation="MATELKMPQMGYDMEEGTVVRWLKEEGSTVTRNEAVAEIETDKA
                     VVEFESDSEGVLLKIVAPEGTIVPVGQTIAVVGSVGEEVSDVSDILIEQATATNDSET
                     ENSDPADDLEQFHETTEVINGPATGRILATPIARRIAEERGISLANVPGSGPGGRITK
                     KDIEEYEPNLASQSATPVPSETTIPKETFTENVEPVPAPFMSGEKQPISRMRQQIARV
                     TVKSKTEKPHFYVSAEINMTDAMSLRRQINDQLKDDGVRLTVNDLIVKACIQALQKYP
                     KFNAYYQDDGIQYNDKINIAVAIADDEGLIVPAIIDCGEKSLRQVSQMIKNLAERSSN
                     GSLSSQEYTGGTFAISNLGMFDVSSFVAIIHPPQSAVLAVGTVSEKPIVVDGEITVGQ
                     VMTATISADHRIVDGAEGAQFLIEVKRLLQSPTSLMF"
BASE COUNT          568 a          347 c          406 g          408 t
ORIGIN      
        1 ccaagccagg caccgatgtg accctggttg catattcagg gatggttcgt gtagcggaag
       61 aagctgctgc aaaactagag gaagataata tttcctcgga agtgatagat ttaaggtgcc
      121 tttctccgtt agacttggat acagttattg aatcggttaa aaaaaccaat agggttgcca
      181 ttgttgagga aacttggaaa acaggtggtt tttcaggaac aatcgctagc gatatacaag
      241 aggcagcatt tgatgaactt gatggtcctg tcataagaat aaatggccca gatgtgccag
      301 cgccctacgc tagaaacata gaacaactta tgatcccaaa ttcagattgg gtagtagaat
      361 cagttaaata taacttcggt ctttaatgga gatgagtcag agatggccac tgaattaaaa
      421 atgccccaaa tgggatacga catggaggaa ggcaccgtag tgcgctggtt aaaagaagag
      481 ggttctacag taactcgaaa tgaagcggtt gcagaaatag aaactgataa agcagtcgtt
      541 gaatttgaat ctgattcaga aggggttctt cttaagatag tcgcaccgga agggaccata
      601 gtgcccgtag gccagacgat agctgtagtt ggcagtgtgg gtgaggaagt gagcgatgtc
      661 tcagatatcc tgattgaaca agcaacggcc accaatgatt ccgaaacgga aaattctgac
      721 ccagccgatg atctggaaca attccatgaa accacagagg tcattaatgg accagccacc
      781 ggccgaatac tggctacccc gatagcacgc cgcatagccg aagaacgtgg cataagcctt
      841 gctaatgtac ccggttcagg accaggtggc agaatcacaa aaaaagatat cgaagaatat
      901 gaaccaaacc ttgcttcaca gtcagctacc cctgttccat cggagacaac aatacccaag
      961 gagacattca cggagaatgt agaaccggta cctgctccat tcatgtctgg ggaaaagcaa
     1021 ccgataagca gaatgcgtca acagatagca agagtcactg ttaaaagtaa gactgaaaaa
     1081 cctcactttt atgtttcagc agaaataaat atgaccgatg ctatgtctct tcgaagacaa
     1141 attaatgatc aactaaaaga cgatggcgta agattgacag taaatgactt gatagtgaaa
     1201 gcatgtattc aagctctaca gaaatatccc aaattcaacg cttactatca ggacgatggg
     1261 attcagtaca acgacaaaat aaacatagcc gtagcaatcg cagatgatga aggattgata
     1321 gttccggcca ttatagactg tggcgaaaaa tcactgaggc aagtatccca gatgatcaaa
     1381 aatttggccg aaagatcgtc aaatggtagc ctgagctccc aagaatacac tggcggcaca
     1441 ttcgctatta gtaacctagg aatgtttgat gtcagcagtt tcgttgcaat aatacatccg
     1501 cctcaaagcg cagtattggc cgtaggtacc gtatcggaga aaccgatagt tgttgacgga
     1561 gaaataacag tcggccaggt tatgacagct actatttctg ctgatcatag aatcgtagat
     1621 ggagctgaag gggcccaatt tttaatagaa gtaaaacgcc tattgcaaag ccctacttct
     1681 ttaatgtttt agctgaaaat ttacttgccc tttaaggcaa tttcctaat
//
LOCUS       DASCNS010000150         8955 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001185118, whole genome shotgun sequence.
ACCESSION   DASCNS010000150 DASCNS010000000
VERSION     DASCNS010000150.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001185118"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="QF838_05365"
     CDS             complement(<1..924)
                     /locus_tag="QF838_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HJO60145.1"
                     /translation="MNILIAGATGFIGKALVSFLKGRGHVLTALVRDVNKAAEQLGTD
                     VKLISFGSTDRELIAEFEDSEVILNLTGRPLAPSRWTKAKKKDFFDSRVGVTKRIVAI
                     MRECENPPSVLVSASATGYYGNRFGEEITESSEKGEGFLADLCDLWEKTAFKAEDFDT
                     RVCALRIGLVLGREGGFLKQLSLPFEMGIGSYIGSGNQWVPWIHYLDLLRIIDLTIND
                     ESISGPINCTSPRPVTGKNFAKALAPILGAKILIRLPRLCLRLVFGEGEKVLTSSQKA
                     YPTLLQEKKFQFAHGDLVHALKEECSPTAVSI"
     gene            1065..1589
                     /locus_tag="QF838_05370"
     CDS             1065..1589
                     /locus_tag="QF838_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60146.1"
                     /translation="MPITSVFFLVNLVGGVAVLGSYCAGLYLYPEHRTALWGGVAGNW
                     KTLFTTSMFFAAAGYLVFCYTMTISDGANESSFLGKYTFIILAAAFLFSASVWMPATL
                     QYLDTKNEIWWIVSVTSLWITAASLTYLGLAFNATEFPHVNLFQNSVTLIGIIWITCH
                     CLVFDAIIWAAKFP"
     gene            complement(2169..2510)
                     /locus_tag="QF838_05375"
     CDS             complement(2169..2510)
                     /locus_tag="QF838_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60147.1"
                     /translation="MISRSEEISCLMCGKTNYLPIKTLAAIPRPEYREELIKSLDKTS
                     FKIKYKIMNRALLSRFPEKGSGTIQPYASCVNISGPTRTLTAAHSRENLKKGFYRATG
                     LRLKDLEEALV"
     gene            complement(2712..3779)
                     /locus_tag="QF838_05380"
     CDS             complement(2712..3779)
                     /locus_tag="QF838_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60148.1"
                     /translation="MGIHIPKFGLNRNDTSSISAFATDVARAEKLGWDCAFLPDSQLR
                     RRDTYVLLSAAAQSTSEITIGTLLANPVTRHPSVTASSIATVAELAPNRTILTMGIGD
                     TAVRLAGLRPARIKELESSVHMMRALLNGEPVDVGAVQPARLPFPQEVPIWLAAGGPK
                     TLEMAGSCADGVFIRVGTDIGNIATSVERIRLGASKSNRDPNAVKIGAVFHTVFYEDE
                     ESSLLMAKSMAAGYYEYSPMLLNNIGLGWEGPHPEQLKKQGKVWPDFHHAPDLIESGR
                     AVGFLSERHADAFCLRGDAHQIANQIVRILEECKELDIEFEYVVLQPIPNPPTPDLGK
                     ESYIEQVPEHILSSVKNSLTK"
     gene            3827..5113
                     /locus_tag="QF838_05385"
     CDS             3827..5113
                     /locus_tag="QF838_05385"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60149.1"
                     /translation="MNLLIISGKNYYTKYLLERVSNNVAKSELALSGIKVLDISNGYA
                     GGYCTKLLRDLGADVIKVEIPKIGDICRSFGPFFNNDPNIETSAPHLYLNAGKKSITL
                     DVTNPEAKDVFLKLCAESDVLVENFKPGLMGQMGFQYQELSTSNPGLIMCSLTYFGQF
                     GPYSNFEGSDIVSYAMSGYMYLTGDEDKEPMKAGGRQAEYQCGLAGSMSIIAALIARS
                     LSGHGDHIDVSATEALTSTFDGVAYFQTYHKTGSDPTRAGTRLITREPTGAYPSTLLP
                     CKDGWVHVHYSPSNPEGIGFLTRNERLADDEIMSRMREHADEIDDLITEWLRDHTREE
                     VQTLAQEVRVPFTMVQSIEETMSDPQNKHRNFFAPIEHEVVGKMSYPTSPFRLKTAEW
                     EALPAPILGQNNHQVYEKDLKLLASKIAGLKSKGVM"
     gene            5114..6361
                     /locus_tag="QF838_05390"
     CDS             5114..6361
                     /locus_tag="QF838_05390"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60150.1"
                     /translation="MATPTPLSNIRVADLTNVIAGPVATRILGHMGAEVLKVELPWGR
                     AIGNIAMHGDEGEGRAYNKVGTFNEVNRAKKSMAIDLTHTTGKKLFKEIVSVSDVVIQ
                     NYSPRVMGNLGLAYEDLRAVRPDIIMVSMPALGLEGPWSNYISFGPGTDALGGLSSIT
                     GYKDGRPHKPGNFYADHNSGFHVATGIMAALFRRYKTGEGQHLEIVLREATMSVIGEY
                     FIEYQFTGREPKRIGNDHPVFFPHGIFQCKGNDSWIAISVESYQEWQALCEVIGSFEL
                     KNDKTLSKVSSRRLNREKIELEITNWTKRQTNMEAMDLMQSTGIRAGIVAKASDILSD
                     AHVSERNYLDTVDHPDAGEYTSPGLPFKFTRSSTNLGVRAPMFSEHSLPILSDLLAKD
                     ISTIESLIEKGTTPLEPIDRVYS"
     gene            6443..7216
                     /locus_tag="QF838_05395"
     CDS             6443..7216
                     /locus_tag="QF838_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO60151.1"
                     /translation="MALIFEKRNKIAYLTINRPEAMNAMDPETYSELSQAWIEIRDDP
                     NIWCAIITGAGDKAFSAGADLKKTIPRNPEKWELWQTQDEQILNRGLEVWKPVIAAVN
                     GYCLAGGMTLLMATDIRLATENASFGLSEVKRGILPGNGGTQRALRQLPYPVAMWILL
                     SGERLSAEEALKYGLINEIVSAPELMSEAEKRASIICENGPLAVRAIKELAVKSQYMQ
                     LEQGLRMEVMMQSLLKTTEDAVEGPKAFTEKRKPIFKGK"
     gene            7241..8413
                     /locus_tag="QF838_05400"
     CDS             7241..8413
                     /locus_tag="QF838_05400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60152.1"
                     /translation="MTERYELPEELRLTRNLVRDFNKNEILPLEREIPHDSITIPEND
                     FLRLSQKTKEMGLWCLGVPEKHGGAGLSLFANAVITEEMVQHAAGLYCPAYGTMGDAP
                     PEIIWAGDEYQIKKYGLPTVEHKLKGWFAITEPSGGSDPARAIQTHAERDGDDWIING
                     SKIFISGSPWGDWGIVFARTDPESRRGITAFIVENDWPGLTVEPVPVMRAYYPGQLFF
                     DDLRVPSKNVLGEVDGGWDLLANKLLARARIPYSASNLGVAVAAHKMAVEYSKTRETF
                     GAPLASRQAIQWMLVDSEIEIRACRWLVWEAAWQFDNGEDFRQAASVAKIHSAETLGN
                     VVDRAIQVHGGMGVTEWLPLERWYREARIRRIGEGPTEVQKMVIARELLNPGGVHA"
     gene            8423..8845
                     /locus_tag="QF838_05405"
     CDS             8423..8845
                     /locus_tag="QF838_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60153.1"
                     /translation="MNIFFDTDYTILGVDGSLRPHVEELFHYLTRKDHNIYIWSGNGI
                     RTKDIEKHQLSNFVTGYFEKPVHDYWANTSKLPTEDQPNMVIDDNLEIVAVLGGIWVN
                     QYYFPNKADIEMKAVKEIIAEIELSGTSEDPRFRKLKF"
BASE COUNT         2781 a         1889 c         1896 g         2389 t
ORIGIN      
        1 gatgctgacc gctgtgggag aacattcctc tttcaaagca tgtactaaat ctccgtgagc
       61 aaactgaaac tttttttcct gcaaaagagt tgggtaagct ttttgactac tcgttaagac
      121 tttttctccc tctccaaaga caagtctcaa acataatcta ggtaatctga taagtatttt
      181 ggctcccagg ataggagcca aggcttttgc aaaattttta ccggtcactg gcctaggtga
      241 cgtgcaattt atcgggccag atatactttc atcattgatc gtaagatcta tgatccgcaa
      301 aagatctaaa taatgaatcc atggaaccca ttggttacca ctgccaatgt aactcccaat
      361 acccatttca aatggtaatg acaactgctt caagaaccca ccttcccttc ctaaaaccaa
      421 accaatccgc aaagcgcaca ctctagtatc aaaatcttca gccttaaaag ctgttttttc
      481 ccatagatcg caaagatcag ccaaaaatcc ttcgcctttt tctgaggatt ctgtaatttc
      541 ttcaccaaat cggtttccgt aataaccagt ggcgctagcc gacaccagaa ccgatggagg
      601 attttcgcac tccctcataa ttgctacaat tctttttgta acgccaactc tactatcaaa
      661 aaagtctttt tttttggctt tcgtccacct agagggagcc agtggacgcc cagtcagatt
      721 aagtattacc tcagagtctt cgaattctgc gatcaactct ctatcagtgc ttccaaaact
      781 gatcaacttt acgtcagttc caagttgttc cgccgcttta tttacatctc gaaccagtgc
      841 ggtaaggaca tgacctcttc cttttaaaaa agaaaccaat gccttgccaa taaatcctgt
      901 agcaccagcg atgagtatat tcaccaaaaa gttccactac atttgcttag tatgccaaga
      961 aaggcgtatc ttggcgcatt ttaagttcat ataattgtat tataacacaa ttatatgtat
     1021 tattaaattt acctctgtat tagcgtgatc aggagatgtt tagaatgcca atcacatccg
     1081 tattctttct agtgaacctg gtgggggggg tagcggtttt gggttcttat tgtgctggac
     1141 tatatctata tcctgaacat cgaacagccc tatggggagg cgtcgcaggg aattggaaaa
     1201 ccttgtttac tacctctatg ttttttgctg ctgccggcta tctcgttttc tgctacacaa
     1261 tgacgatttc agatggagct aatgagtctt cattcttagg aaaatacacc ttcatcatcc
     1321 tcgccgccgc ctttttgttc tcagcctccg tatggatgcc agctacatta cagtacctcg
     1381 atactaaaaa tgaaatatgg tggattgtct ccgtgacatc actgtggata acagctgctt
     1441 ctttgacata tttggggctg gccttcaatg ccactgaatt tcctcacgtg aatctgttcc
     1501 aaaattcagt gacattgata ggaataattt ggataacgtg ccattgccta gtatttgacg
     1561 caatcatttg ggcagccaag ttcccataaa acatatttgt tccatataga ggtaaaaatg
     1621 gccccaatat acaaatctaa aatggattct tttgtagcca aacaattacc caaataaaaa
     1681 acggtgacca actactatat tgaatcaatg tttgaaattt ttacacctga tcaaatagtt
     1741 gaacgtactg gtttgacttc ggatgaggtc ataaattctt tcagattatc attgagaaag
     1801 gaatctttga cggaacagta ctcggaaaca tgcgagataa gagttgaaat gttttctgcc
     1861 cactggtcga agaattatgt cgttggctct tggtaacgag acgaaaaggg ccacgttgac
     1921 cctttcaaaa acatactctc agatttcgga gttgatagaa tttgatttcc gatcagataa
     1981 attaagcaaa aagcaccaca ggctacaagt tacaccatga ggcaggtttt agtccgatag
     2041 gtttaactac atatgggatg atgcaagagt gtattaccga taatcagtac ggacttttct
     2101 atctccatgc tctcattttc aaagaaatgt tggatgcaat cggcacaaaa gcttcccgaa
     2161 ggcaggattt aaactagagc ctcttctaag tcttttaatc tcagtcccgt agctctataa
     2221 aaaccttttt ttaaattttc ccttgaatga gccgcagtca atgttcgagt gggtcctgaa
     2281 atattcacgc aagaagcata gggttggatc gtaccagacc ccttttctgg aaatcgtgat
     2341 aacaaagcac gattcatgat tttgtatttg atcttaaatg acgtcttatc caaggattta
     2401 attaattcct ctctgtattc tggtctagga attgccgcca atgttttgat gggtaaataa
     2461 ttagtcttac cacacatcaa acagctgatt tcttcagacc tcgatatcat tttacctccg
     2521 caatgtgaac agtcggaagc gcgtgtcgtc aatttaatgc tattttctat caaggaagtt
     2581 tctctaaagt attaatacat acacttttca ccaatttggc aaatatgtca aatatgatgt
     2641 aataaagtac acatacccct agttagtcat acatcaaaag tagattacca ctcataaaaa
     2701 caaattgtaa atcatttagt gagagagttt tttacagaag aaagaatatg ttcagggacc
     2761 tgctcaatat atgactcttt gcctaaatct ggtgtcggag gattaggtat tggctgcaaa
     2821 acaacatatt caaattcaat gtctagttcc ttgcattctt ccaaaattcg aactatctga
     2881 ttggcaatct gatgagcgtc acctcttaga cagaaggcgt cggcgtgcct ctcacttaaa
     2941 aaccctaccg cccgtccact ttcaattaaa tcaggtgcgt ggtgaaaatc aggccaaact
     3001 tttccttgtt tttttaattg ttccgggtga ggaccttccc agcccaggcc gatattattc
     3061 agtagcattg gcgagtattc gtagtatcca gcggccatag atttagccat caaaagagag
     3121 gactcttcgt cttcataaaa taccgtatgg aaaaccgctc cgatttttac tgcatttggg
     3181 tcacgattag attttgaggc tcctaatctt attctctcaa ctgaggtcgc aatattccca
     3241 atatcagttc cgactcttat aaagacccca tctgcacaag agcctgccat ttccaaagtt
     3301 ttggggcctc ctgctgcaag ccatattggt acctcttggg gaaatggcaa tctggcaggt
     3361 tgtactgctc ctacatctac gggttcccca tttaataatg ccctcatcat gtgaacacta
     3421 gattccagct ccttaatccg ggcagggcga agaccagcta atcgcacggc tgtatctcct
     3481 atacccatgg tcaaaatagt cctattggga gctaattcag ccaccgtagc tatactcgaa
     3541 gctgttaccg aaggatgtcg agtcactggg tttgctagca atgtaccaat ggttatttct
     3601 gacgtagatt gagccgcagc tgaaagcaaa acatacgtgt ctcgccgccg taattgagaa
     3661 tcgggaagaa atgcgcaatc ccaacctagt ttttctgccc tcgctacatc agtggcaaaa
     3721 gccgatattg agcttgtgtc gtttctgttc aagccaaatt ttggtatgtg gatacccatt
     3781 aataagcctc ctcggattca aaactttact cggattataa tgtcagatga atttacttat
     3841 cattagtggt aagaattact acactaagta tctcttggaa agggtaagca ataacgtggc
     3901 aaaatctgaa ctagctttaa gcgggataaa agtattagat attagtaatg gctacgccgg
     3961 gggttattgc accaagttgt taagagacct cggggccgat gttattaaag tcgaaatacc
     4021 gaaaattggt gatatatgca ggtcttttgg tccattcttt aacaacgatc cgaacataga
     4081 aacgagtgcg ccgcacctat accttaatgc aggtaaaaaa agcatcacct tagatgtcac
     4141 aaatccagaa gccaaagatg ttttcttaaa attgtgcgct gagtcagacg tacttgttga
     4201 aaatttcaaa ccgggactaa tgggtcagat gggatttcaa taccaagaat tatccacatc
     4261 aaacccaggg ttaataatgt gttctctaac ctatttcgga caatttggcc catacagcaa
     4321 ctttgaaggt tcagacattg tgtcgtacgc gatgagcggc tacatgtacc tgactggaga
     4381 cgaggataaa gaaccaatga aagctggcgg tagacaagct gagtaccaat gtggattagc
     4441 cggttcaatg tccataatag ctgctctcat tgccaggtca ctatcgggcc atggagacca
     4501 tatagatgtt tcagcaactg aagctctaac tagtaccttt gatggagtcg cttatttcca
     4561 gacctatcat aaaaccggtt ctgacccaac tagggctgga accagactca taactcgaga
     4621 accaacagga gcctatccat ctactctatt accttgcaaa gatggatggg tacatgtcca
     4681 ctattctccc agtaatccag agggtatagg tttcctgaca agaaacgaac gattagcaga
     4741 tgatgagatt atgagtagaa tgagagaaca cgctgatgag attgatgacc ttataaccga
     4801 atggttgcga gatcacacgc gtgaggaggt tcaaactcta gctcaagaag taagagtacc
     4861 cttcactatg gtccagtcaa ttgaggaaac catgtcagat ccccaaaata agcacagaaa
     4921 cttctttgcc ccaatagagc atgaagtagt aggtaaaatg tcctacccta cctctccatt
     4981 tcggctaaaa acagctgaat gggaagcatt acctgctccg atacttggtc aaaataatca
     5041 tcaagtatat gaaaaagatc taaaactcct cgcctccaaa atagctgggc tgaaaagcaa
     5101 aggtgttatg taaatggcaa cccccactcc cttatccaac atcagggttg ctgaccttac
     5161 caatgttatt gctggccccg tagcaacccg aatactgggc catatgggcg ctgaagtact
     5221 aaaagtggaa ctgccttggg gaagggccat tggcaacatt gctatgcatg gtgacgaggg
     5281 agaaggcaga gcatataaca aggttgggac attcaatgaa gtaaacagag ccaagaaaag
     5341 tatggctata gatttgacac acactacagg taagaaattg ttcaaggaaa tagtaagtgt
     5401 cagtgacgta gtgatccaaa actacagtcc aagagtcatg ggaaatttag gtctcgcata
     5461 tgaagatctt agagcggtaa ggccggatat aataatggta tctatgccgg ctttaggact
     5521 tgaagggccg tggagcaatt acatttcatt cggaccaggt acagatgcgt tgggagggct
     5581 atcttcgata acaggataca aagatggccg gcctcacaag ccaggtaatt tctatgcgga
     5641 tcacaattct ggatttcatg tagccactgg aataatggca gctcttttcc gtagatacaa
     5701 aacaggtgaa gggcagcacc tagagatcgt cctcagagaa gcaacaatgt cagtgattgg
     5761 tgagtatttc atagagtacc aattcaccgg aagagaaccg aaaagaatcg gtaacgatca
     5821 tccagtcttc tttccacatg gaatattcca atgtaaaggt aatgattcct ggattgctat
     5881 ctcagttgag tcatatcagg aatggcaggc attgtgcgaa gtaatcgggt cctttgaatt
     5941 gaaaaatgac aaaaccctat caaaagtgag ttcgaggaga ttaaataggg aaaaaataga
     6001 attagaaata actaattgga ctaagagaca aactaacatg gaagctatgg atttaatgca
     6061 atccaccggt atacgcgctg gaattgtggc taaagcctct gacatactct ctgatgctca
     6121 cgtctctgaa agaaattatc tagacactgt tgatcatcca gatgcaggtg aatatactag
     6181 cccaggcctg ccttttaaat tcactcgatc atcgaccaat ttgggtgtga gagctccgat
     6241 gttctctgag cacagtctgc ctatactttc cgacttactc gcaaaagata tttcaacgat
     6301 tgagtcgctt atcgaaaaag gcaccacccc acttgaaccg atcgataggg tctactcata
     6361 gataaaaatt gacgtatttt acatcaaatt cgatacaata attcatatca tttaaatctc
     6421 acccaaacaa agggaagaaa cagtggcttt aatatttgaa aaacgaaata agattgccta
     6481 tttaaccatt aacaggccag aagctatgaa cgctatggac ccagaaacat acagcgaatt
     6541 atctcaggct tggatagaaa ttagagacga tccaaatatc tggtgcgcga taatcactgg
     6601 tgcaggagac aaagcattct cagcaggagc agatttgaaa aaaactatcc caagaaatcc
     6661 tgaaaaatgg gagttatggc aaactcagga cgagcaaatt ctgaacaggg gtttggaagt
     6721 atggaagcct gtaatagctg cagtcaacgg atactgccta gcaggcggaa tgacactctt
     6781 aatggcaaca gacatcaggc tagcaacaga aaatgcctca ttcggccttt ctgaggtaaa
     6841 aagaggcatt ttacccggta atggtggcac ccaaagagca ttaagacaat taccctaccc
     6901 tgtcgccatg tggatattgc taagtggaga acgtttatct gcagaagaag cccttaaata
     6961 cggcttgata aacgaaatag tctccgcccc agaactgatg agtgaagcgg aaaaacgagc
     7021 atcaataatc tgtgagaacg ggcctttagc agtcagagct atcaaagaac tggcagtaaa
     7081 aagccaatac atgcagctag aacaagggct tagaatggaa gtaatgatgc aatctctctt
     7141 aaaaaccaca gaagatgccg tggaaggccc gaaagctttc acagagaaaa gaaagccgat
     7201 attcaaaggg aaataacatt aactaaggta ccaaaccaat atgacagaaa gatatgaact
     7261 acctgaggaa ttacgactaa caagaaacct agtcagagat ttcaacaaaa atgaaatatt
     7321 acccttggaa cgagaaatcc ctcacgattc cataaccatc cctgaaaacg attttttaag
     7381 gctttcccag aaaactaagg aaatgggtct atggtgctta ggtgttcctg aaaaacatgg
     7441 tggggcaggt ctttctttat ttgcaaatgc agtcattacc gaggaaatgg ttcagcatgc
     7501 tgccggtctc tattgtcccg cgtatggaac aatgggtgat gcccctcccg aaattatatg
     7561 ggctggagac gaatatcaga taaaaaaata tggcctcccc actgtggaac acaagttaaa
     7621 aggatggttc gccattacag aacccagtgg tggctcagac ccggccagag ccatacaaac
     7681 ccatgctgaa agagatgggg acgactggat cataaatgga agtaagatat tcataagtgg
     7741 atccccatgg ggggattggg gaattgtgtt cgcacggacg gacccggaaa gcaggagagg
     7801 gataacagcc ttcatagtgg agaatgattg gcccggcctt acagtggaac cggtacctgt
     7861 aatgagagcc tattatcctg gccagttgtt ttttgatgat ctacgggtac caagtaaaaa
     7921 cgtattaggt gaagtcgatg gaggatggga tctacttgca aataaattat tggccagagc
     7981 ccgaatacca tattcagcat ccaacttagg cgtagctgtt gccgcccaca aaatggcagt
     8041 ggaatattca aaaacaaggg aaacttttgg ggccccattg gcatcccgac aggcaattca
     8101 atggatgcta gttgatagtg aaatagaaat aagagcttgt cggtggcttg tttgggaagc
     8161 tgcctggcaa ttcgataatg gagaagactt cagacaagca gcttctgtag ctaagataca
     8221 ttctgctgaa accttgggta atgtagttga ccgagcgata caagttcacg gaggaatggg
     8281 tgtaacggaa tggctcccct tagaacgatg gtacagagag gcgcgaatcc gcagaatcgg
     8341 agaaggacca actgaggttc aaaaaatggt tatcgctagg gaacttttga accctggtgg
     8401 ggtccatgca tagcactgtc cattgaacat attttttgac accgattaca caatattggg
     8461 agtcgacggg tcactcaggc cccatgtaga agagttattt cactacctga cccgtaaaga
     8521 tcacaatatt tatatttggt ccggcaatgg tattcgcaca aaggatatag aaaaacatca
     8581 attgtctaat ttcgtaacag gatattttga aaaacccgtt cacgattatt gggcaaatac
     8641 ctccaagttg cccacagaag atcagcccaa tatggtcatc gacgataatc tggaaattgt
     8701 tgctgttttg ggggggatct gggtaaacca atactacttc cctaacaaag ccgatatcga
     8761 gatgaaagca gtgaaggaaa taattgcgga aatcgaatta tcaggcactt cggaagatcc
     8821 acggtttcga aaactcaaat tctaaaacga caatcttgct gacgctctag aaaatatcga
     8881 tatcaggaaa cgatatcgat taacttcatc ataatccaac cagccaccga cattaaggcg
     8941 acgagaaaaa gggat
//
LOCUS       DASCNS010000151        14030 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001186298, whole genome shotgun sequence.
ACCESSION   DASCNS010000151 DASCNS010000000
VERSION     DASCNS010000151.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 14030)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14030)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14030
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001186298"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="QF838_05410"
     CDS             complement(<1..213)
                     /locus_tag="QF838_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HJO60154.1"
                     /translation="MTQTSEENSNSGVQRLKRIAVIGCGSLGTQIAIELSSSGNIVIV
                     IDKDPKSFFSLPSHLLETSRVVTAVGD"
     gene            complement(221..634)
                     /locus_tag="QF838_05415"
     CDS             complement(221..634)
                     /locus_tag="QF838_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HJO60155.1"
                     /translation="MILAVITGSTSDEYVTATASRLAKQMKFPVNLLYVIEVERNYPI
                     DMEIPQDTEKGEKTLSQMEKIVSKFKVKSKGEILQARFSGSAIISQSENIQARLIVLE
                     YKERNSDNLYDNICGYVAGNAKCDVIMCTNTGKLA"
     gene            complement(675..1361)
                     /locus_tag="QF838_05420"
     CDS             complement(675..1361)
                     /locus_tag="QF838_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HJO60156.1"
                     /translation="MANKQVAVIGLGRFGVSTTRTLYNLGHDVLAIDKDEARVQSVLG
                     QCTYAVSAECTNESALRDLGIQDYDVAIITIETNIADSVMACVLLKTIGVKTIVARAR
                     DSLHASTLRRIGVDRVIQPEEEMGTRLAHSLFNASVEEYLEITNNYGVSRYRVPEKLS
                     GTTLEEIGFTNSRDSYGITAVALCKGRKVTLNPPVSSKVESGDFVIVAALDSDLETLD
                     DSSGGGEEQI"
     gene            complement(1392..2822)
                     /locus_tag="QF838_05425"
     CDS             complement(1392..2822)
                     /locus_tag="QF838_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014443.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HJO60157.1"
                     /translation="MDTRFNKRLGDIFITKPRRSEIAPITFNVDERRINARTFPSPLI
                     LVAVFLALILVGALLLSAPFAHHEQGWGDPVLSIFTATSAVTVTGLIIVDTATYWTSS
                     GQVIILLLMFVGGLGFMTLAAFLLAVLGQRVSMAQRLLIRETLGSENMGGIQRLSVTI
                     VAAAISIQVIGFFALFARFLFLESPGEAAWQALFHSVSGFNNAGFVILNQSDGLHAFR
                     EDFIVLSIIGWLIILGSLSFWVVLDLVSKDSLRKFSLVTKIVLLMTVALIITGASFFL
                     FAESNNPKTIGDLPINAQIGVSIFEGVSGRTAGFSTIDYGYARPATDVFMSLLMFIGG
                     ATGSVAGGIKVTTFFIIVLSVVAIIRERAHVTAFGREISPTTVRRSYAVTLICLGFIF
                     LCTLCLALTNSHILIRDLAFESVSAFGTVGLSTGATEELNRLGHVIVAITMFIGRVGP
                     IIVGLRMVPNREASPYRYATEPVTIG"
     gene            3065..3661
                     /locus_tag="QF838_05430"
     CDS             3065..3661
                     /locus_tag="QF838_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HJO60158.1"
                     /translation="MRAPKGFHMPHPDIHLPHLILPHGFLPHILDQYIHSRWNHYFLQ
                     CCLASVSLILILLIGDTLKTAIVVGIASSAFTIFVVPNSVAATPRKVIGGHLLAAFIG
                     TLIALILQSPPLIQQVLENRYFLDVAAALSVGIGIFLMVVTNTEHPPAAGTSLGLVIQ
                     CCEPNTGVDWSSIIFIIASAGLLSGVRIALRDKMVNLL"
     gene            3798..4943
                     /locus_tag="QF838_05435"
     CDS             3798..4943
                     /locus_tag="QF838_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60159.1"
                     /translation="MNSDLEYSITRIHNSKTKLVMSVSGVGSQSINWLLSVPGASKTL
                     IEATIPYSNESLNRYIGEVPRQYVSKTTALSMAKAAYIQGVQYGNHEIDIIGVSCTGA
                     ISTNRKRRGDNQAFIGLWGPRLKYVAHLILEKGERNRVEEEELVSSLIVQYIEEKLLG
                     NSVLNMELNKLESVSVDETEFPSDLDSLVNGHISSITYMGSNLVGLDKSFKGGILSGS
                     FNPMHQGHIELSKLASDMLGVPVAFEISASNVDKPPLQANEIEKRVSQFERSETVILT
                     CAPLFGEKSSIFQNSTFIIGNDTALRLVDPKYYDNDVQNMYTSLQKVKDNKCDFLVAG
                     RLQNSEFKTISDMAIPEAFISLFQGIPESQFRMDLSSTELRSDGTKL"
     gene            4946..6400
                     /locus_tag="QF838_05440"
     CDS             4946..6400
                     /locus_tag="QF838_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HJO60160.1"
                     /translation="MSYKNLSEFISFLDSKNQVQYIDAQVSRDLEISEVTDRAVKSGG
                     PVLYFRNVEGFTIPVVTNLFGTHQRMAWALGVENTDDLTQKVRDILGIVKTPPQSMLD
                     KLKTLKDMASLARTQPKRISSAPCQEIVKTGQDANLNHLPHLTCWPMDGGRYITLPLV
                     ISKDPSSGKRNVGTYRMQIYDSHTAGMHWQSHKVGASHYRSGELKKLPKMEVAIALGS
                     DPVTMWTGALPLPPDMDEIAVSGVLRNSSVEMVKCKTVDLEVPAHSEAVLEGYIIPGE
                     LREEGPFGDHTGYYSLEDNYPVFHLTALTHKRHPIYPATVVGRPPSEDFFMGKAVERL
                     MLPALQLTLPEVVDINMPAEGVFHNLVIVSMKKEYPGHTRKVMNAIWGLGLMMLAKGI
                     VIVDDTVNVHDLSEVAWRVTSNINPDKDIVITEGPVDDLDHASPTPKYGSKIGIDATK
                     KTALDGRERIWPPDVVMSEEIKLLVTNRWSEYGF"
     gene            6406..7272
                     /locus_tag="QF838_05445"
     CDS             6406..7272
                     /locus_tag="QF838_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HJO60161.1"
                     /translation="MPLNRFWPKIPNFFDAIKFQESLFALPFAYIGMLLAIRGLPDFQ
                     SFFWITLAMVSARTVGMIANRIIDRHIDSKNPRSSQRHLPRGILSVTDLLVPGIISSL
                     IFMLCAYMLNTLALILAPVALIYLVVYPFTKRVTWLANLLLGWALAIAPAAAWIGMLG
                     KLGWQPVLLSTSVALWAGSFDIIYHTQDVSFQKKEKLHSVASRFGISKAFKIAQCMDL
                     LAVLCLIILGAWMQLGIFYYGGCGLSTIFLVYKYILVKPSDLSKMGMSFMRINAFVSC
                     YMLLGTIASLIV"
     gene            7275..7874
                     /locus_tag="QF838_05450"
     CDS             7275..7874
                     /locus_tag="QF838_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HJO60162.1"
                     /translation="MEKLEARIVVGISGASGSLLAAKTVDALLDRKVGVSLIASNPAR
                     MVWQHEMSMSYGESIEEWSSNNNFEHFAIGDFKAPIASGTYPTTGMAIVPSSMATISA
                     VAKGLSDNLIRRAADVCLKEKRPLAMVPRESPLNAIHLGNMKYLATLGVTILPSDPPY
                     YLPITTLEEVANFTAQRILIALGITDQLPEEMQYRGPII"
     gene            7941..8507
                     /locus_tag="QF838_05455"
     CDS             7941..8507
                     /locus_tag="QF838_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO60163.1"
                     /translation="MISAIEAVKAISDIRSSEVVVATMTANRYWESVSQNQEMDLPIF
                     GAMGKASSVALGLALSQPNKKIVVLDGDGSLLMNLGSLVTVAGMEPKNFLHFVFEDGA
                     YWTTGGQPIPGNGKFNLSAMANDAGYKSSHSFEDLETLVSELPSLMRQDGPIFVSIKI
                     THPEHAGEPFIGSTKNAMEKLSKSISGY"
     gene            8511..9170
                     /locus_tag="QF838_05460"
     CDS             8511..9170
                     /locus_tag="QF838_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HJO60164.1"
                     /translation="MYDFHTHTFLSDGVLSPIELIRRAIAIGYKTMAITDHVGPGNLE
                     FVLATLKKDCEMAEKRWDIKVYPGVEITHTPTEDIHLLAKEARSIGAKIVNVHGETIM
                     EPVIPGTNIAAVSSEYVDILAHPGLITVEEAKMAAKNGVFLEISGRKGHGLANGHVVK
                     TARESGATLILDSDAHAPEDLMTQEYAEKVAAGSGLNETERATLLEQTPLSLVSRVNS
                     R"
     gene            complement(9172..10152)
                     /locus_tag="QF838_05465"
     CDS             complement(9172..10152)
                     /locus_tag="QF838_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HJO60165.1"
                     /translation="MNDQVVFWAPDDSGLQVLKDAMPEGVEIDWVNSSGSLEESTEAL
                     QNTKAIILGGALVFDLELAAKCPELRLIQTTSAGTNQLDKAALGELGIKVSNNGGGNA
                     VAVAEHTIALMIGVYRKLQLQFKSVQDGKWMSNIRIDWFSQAHELTGKTVGIIGVGRI
                     GSRVAKRLQGWECELIYHDVVDPDSELVSDLSMEKVGLDELLSTSDIITLHVPLNRNT
                     RGMISDREFDLMKPTSILINACRGPVVDEDAFIRAIEQNKIMAAGVDVLEVEPTPEDN
                     PLIDMDNVLITPHLAAFTQEASDKSRVFATYNTAKVVNGGDPESVVLPDD"
     gene            10287..11489
                     /locus_tag="QF838_05470"
     CDS             10287..11489
                     /locus_tag="QF838_05470"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60166.1"
                     /translation="MKPQALKNVRILDLTHYISGPYCTKLLGDFGADVIKIENPLVGD
                     PCRKMPPFDETNPNSNDSLLFQYLNTNKKSVELDLKSDFGHLAAEELVRNSDILVENF
                     RPGTLASYGLTESKLRTLNPNLIVVSISNFGQTGPYRDFEATDLVFYALGGLMFIFGS
                     INKSPLKHAFHQAQFKAGTNAASAALMAFYEMINSGTGQKVDISIQESMAAAVRDTTT
                     SYAASGIVRTRQTLMNGELPRAPIQVKDGYIVPITFGSTDWNKTANLLGDESLADHKF
                     QTPEGRRMHAEEFENLVKAAFIRRGKHELFYGAHSHRELVYGLVQDAADLIENPQYQH
                     DQYFQAIGNSGSDTPKFPGRPFKLSKTPWNIQTTAPALGQHTEEILFNKLNLPIDKFQ
                     NTGRSNEK"
     gene            11479..12828
                     /locus_tag="QF838_05475"
     CDS             11479..12828
                     /locus_tag="QF838_05475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60167.1"
                     /translation="MKNNGILNGIRVVELGQLIAVPYATKLLSDMGAEIIRLESAQRP
                     DAYRLADFYDEREDGKYWNRAINFNEQNRNKLSLGLELDHESSLEYLHDLISISDVFA
                     CNFTPRVMKKFGLEYEHLQKIRPDIIVISSTGYGHTGPWSSFGAIGFGTEAASGLAAA
                     TGYENGPPVQPEIPYPDYTAAEHTVFAVMAALIHRLKEGEGQFIEISQAECVTSTIPE
                     TILEYTANKKIVKRMGNSHPNISPHGCYPCKGQDKWITITANSQDQWKSLSNLLLPET
                     GTDNRFESNESRIHNRKELDKLISSRTLSWDNVVLMNTLQRKGVAAGAVLNGKELLFN
                     EHLNQRNFFEIVDHHPESEVPTLPYPGKPWKMSANSDVKMKGAPLFGQHNESILKNHL
                     GLTNESYDALINEEAVVNYIKLDPKEILVSREAREEEGSIYSYDPDFKSTIKSYFNI"
     gene            12860..13525
                     /gene="cmk"
                     /locus_tag="QF838_05480"
     CDS             12860..13525
                     /gene="cmk"
                     /locus_tag="QF838_05480"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HJO60168.1"
                     /translation="MKFQIAIDGPVASGKTAVGRGVSKALKWNFLDTGIMYRAATRSI
                     LDSGISFEENSSIIETVKKTTLDIDNSPGFEKILINGKDKTSTLKTPEIDRAVSIISK
                     IPEVRKELVRQQRGIASQGPIVMVGRDIGSVVLPEAELRIYLEANVEIRAQRRYQEQI
                     ASKGKLSLNDVKVDLEKRDLIDTQRQDSPLSKYDFYTIIDTTTMNLEDVVDEITSIYR
                     SQI"
     gene            13525..>14030
                     /locus_tag="QF838_05485"
     CDS             13525..>14030
                     /locus_tag="QF838_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013702.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HJO60169.1"
                     /translation="MLYPTCNFLQKITLKCFAKFEVSGKENVPPVGPLIVVSNHLSNI
                     DPSILAVSVSRRLQFLAKSSLFTGPFLSKLLHWYGAYPLNRDKIDVTAFRWTLNQLRQ
                     DGAILIFPEGTRSKGSMIKAKPGVARLVQMSDATILPVAITGTEHIGNVVRVVNPTGN
                     IKVNIGSP"
BASE COUNT         4389 a         2959 c         2827 g         3855 t
ORIGIN      
        1 atctccaaca gcagtcacaa ctctggaggt ttcaagtagg tgagacggca gtgaaaaaaa
       61 tgacttcggg tctttatcaa ttacaattac gatattcccg gacgatgata attctatcgc
      121 gatttgagtg cctagtgaac cacaaccgat gacagcaatc ctttttaatc tttgcacacc
      181 agaattcgag ttttcctctg aagtttgagt catttaattc ctaagccaac ttacctgtat
      241 ttgtgcacat aattacatca cattttgcat tgcccgccac gtatccacaa atattgtcgt
      301 acaaattgtc agaattccgt tccttatatt ctaggactat aagccttgct tgaatattct
      361 ctgactgact aataattgca ctgcctgaga atctagcttg gagtatttcc cctttagatt
      421 tgactttaaa tttactcact atcttttcca tttgggatag agttttttca cccttttcgg
      481 tatcctgagg tatttccata tcgatcggat aattcctttc gacttcgatt acataaagta
      541 aatttactgg aaatttcatt tgcttggcca accgtgaagc agtggcggtt acatactcat
      601 cactggtgga acctgttatt actgcaagga tcatatggcc atctcttcaa taacaaattt
      661 aactttttgt taagttaaat ttgttcctct ccaccacctg aagaatcatc caaggtttca
      721 agatcgctat ccaaagctgc aacaattaca aaatctccag attcgacttt agaacttaca
      781 ggtggattca aggtcacttt ccttccttta cataaagcca cggcagttat tccataactg
      841 tcacgactat tagtaaatcc tatttcttca agtgttgtgc cagacagttt ctctgggact
      901 ctgtatctac taaccccgta attattggtt atttccaagt attcttcaac actggcatta
      961 aacaggctat gcgcgagtct agttcccatc tcttcctcgg gctgaataac cctgtccact
     1021 cctatcctcc tgagagtgct agcatgcaat gagtcgcgag ctcgagccac aattgttttt
     1081 actccaatag tcttgagtag aacgcaagcc atgacactat ctgctatgtt ggtttcaata
     1141 gtgattatcg ctacatcata atcctgaatt cctagatctc tcaatgcact ctcattagta
     1201 cactctgcag atactgcgta ggtgcattga cccaacacgg actgaaccct ggcctcatct
     1261 ttatcaatag ccaaaacatc atggccgaga ttatagaggg tacgagtggt actaactcca
     1321 aatcttccta gacctataac tgcaacctgt ttgttagcca taaaatttac cttcgacgtg
     1381 catttaatac gctagccaat tgtaactggt tccgtagcat accgatacgg gctcgcttct
     1441 ctattaggaa ccattctaag gcctactata atcggtccaa ctcgaccgat gaacatagta
     1501 attgcaacta tcacatgccc caatctattt agctcttcgg tagcaccagt actcagccct
     1561 actgtaccga aagctgaaac agattcaaag gctaaatccc ttattagaat atggctgtta
     1621 gtaagggcca aacataaagt acataggaat ataaaaccca gacaaattag tgtcacagcg
     1681 taagatctcc gaactgtggt cggagatatt tctctaccaa atgctgtgac atgtgctctt
     1741 tctctaatta tggctaccac gctgagaaca ataatgaaaa aggttgttac cttaataccg
     1801 ccggcgacag acccggtcgc acctccaata aacatcaaaa gagacataaa aacatcagtc
     1861 gcaggcctag catacccata atctattgtg gaaaatccag ctgtacgacc actcacaccc
     1921 tcaaaaatgg acacaccaat ctgagcattt atcggcaaat ctccaatcgt tttaggattg
     1981 ttactttcag caaaaagaaa gaatgacgcg ccagtaatta ttaatgctac cgtcatcaat
     2041 aatactattt tggtcactaa agagaatttt ctaagcgaat ccttcgatac caagtcaagg
     2101 actacccaaa aacttaaaga tcccagaata atcaaccacc ctattatgct taaaacaata
     2161 aaatcttctc tgaatgcgtg caagccatca gattggttca aaataacaaa ccccgcatta
     2221 ttgaatcctg aaaccgaatg aaacaaagcc tgccaagcgg cttccccagg agattccaaa
     2281 aataggaacc ttgcgaacaa agcaaaaaaa cctataacct gaattgatat agcagcggca
     2341 actatcgtaa cgcttaacct ctgaataccg cccatatttt cgcttcccaa ggtttcccta
     2401 atcagcaatc tctgagccat tgaaactctc tgaccgagaa cagccaacaa gaaagcagct
     2461 agagtcatga atcccagccc tcccacaaac ataaggagca atataataac ctgaccactt
     2521 gaagtccagt aggtggcagt atccactatg atcaagcctg taactgtcac ggcagaagtg
     2581 gcagtgaata ttgataaaac agggtccccc cacccttgtt catggtgagc aaatggagcc
     2641 gacagtaaaa gggctcccac taatatcaaa gccaaaaaca ccgcgaccag tattagggga
     2701 gagggaaaag tacgggcatt tattcttctc tcatctacat taaaagtgat aggagctatt
     2761 tctgatcgcc taggttttgt tataaaaata tcgccaaggc gtttgttgaa cctagtatcc
     2821 attttatact tctatgtgag atggaccgtg aataattata aaagaatttt gataatctgc
     2881 taatagttta tatttcaaat tagcctctaa ttatagcagt ctgctaaaac agggcatttg
     2941 aaaggtttat atattccttt aattgaggag aatgtagccc aaatttggct attcagaccc
     3001 taaaaacacc gatatccttg aaagataaat ttaagaaaca ggaaatgtcg ggagtaaaaa
     3061 acaattgaga gcacccaaag gctttcacat gccacaccct gacattcacc tacctcactt
     3121 aatccttcct cacggcttcc taccgcacat attagaccaa tacattcatt ccagatggaa
     3181 ccattatttt ttacaatgct gccttgcctc ggtatcactg atactgatat tactaattgg
     3241 agatacctta aaaaccgcaa tagtggtcgg aattgcatcc tctgccttta caatatttgt
     3301 agttccaaac agtgtggccg caacaccacg caaagtaatt ggggggcatc tcttagcagc
     3361 ctttatagga accctaatcg ccctgattct tcaatctcca cctttgattc aacaggtatt
     3421 ggaaaatcgt tatttccttg atgtagcggc agctctatcc gttgggattg gcatattttt
     3481 gatggttgtt accaacactg aacacccacc ggccgcagga acctctcttg gtttagtaat
     3541 ccaatgctgt gaaccaaata caggggttga ttggtcctcc atcatattca taatagctag
     3601 tgccggcttg ctttctggag taaggatcgc cctccgtgat aaaatggtga atcttcttta
     3661 aaaggcatct tgaaagatct ctatatacat ataaatatgt ctttgctgta aaacttaagc
     3721 atcgcaaagc catttgtgaa atgacggcga ttccatacca gcacatggat atctaacagg
     3781 ttaggcacac tagatatatg aattccgact tagaatattc cattactcgc atacacaatt
     3841 cgaagacaaa gttggtcatg tctgtatctg gagtaggctc ccaatcaatt aattggttat
     3901 taagcgttcc tggcgcatcg aaaactttga ttgaagcgac aattccatat tccaatgaat
     3961 ctctcaatcg gtatattgga gaagtgccca gacaatacgt gtctaaaaca acagcgttat
     4021 cgatggcaaa agctgcatat atccagggag tccaatatgg caatcatgag atagacatta
     4081 ttggagtttc atgtacagga gcaatatcca caaatcgaaa aagaagaggt gacaatcagg
     4141 cgtttattgg attatggggc cccagattaa aatatgtcgc ccacctaatt ctcgaaaaag
     4201 gagaaagaaa tagggttgag gaggaagaac ttgtgagttc actaatcgtc caatacatag
     4261 aggaaaaact actaggtaac agtgtattaa atatggaatt aaacaagttg gagtctgttt
     4321 ctgtagatga aacagaattc ccctctgatc ttgattcttt agtgaatggc catattagtt
     4381 ctattactta catgggctct aatttagtgg gattagacaa aagtttcaaa ggtggaatat
     4441 tgtctgggtc ctttaatcca atgcaccaag gacacataga gctttcgaaa cttgcatctg
     4501 atatgttggg agttccagtg gcgtttgaaa tatctgcatc taatgtggac aaaccaccac
     4561 ttcaagcgaa tgaaatagaa aagagagtat ctcaatttga aaggtcagaa acagtgattt
     4621 tgacttgtgc gccattgttc ggggaaaaat ccagtatatt ccaaaattcg accttcatca
     4681 taggtaacga cactgcccta cggttagtgg accccaaata ctatgacaat gatgttcaaa
     4741 acatgtacac atccttgcag aaggtaaagg ataataaatg cgactttcta gtggcagggc
     4801 gtctccaaaa tagcgaattt aaaactataa gtgatatggc gatacctgaa gcatttattt
     4861 cgttatttca aggtataccc gaatcccaat tcagaatgga tttatcttcc acagaacttc
     4921 gcagcgacgg aactaagtta tagaaatgag ctataaaaac ctgagcgaat tcattagttt
     4981 tttagattct aaaaatcaag tgcaatatat agacgctcag gtgagtaggg atttagaaat
     5041 tagcgaggta accgataggg cagttaagtc aggaggccct gttctgtatt tccgtaatgt
     5101 agaaggattc acaatacctg ttgtgaccaa cctatttggt acacaccaac gaatggcttg
     5161 ggctttgggt gtagaaaata ctgatgattt aacgcagaaa gtccgtgaca ttctgggaat
     5221 tgtaaaaaca cctccccagt caatgttaga caaactaaaa actctcaaag acatggcaag
     5281 cctggcccga acacagccca aaagaatttc atctgctccc tgtcaagaaa tagtaaagac
     5341 ggggcaagat gctaatctaa atcatttgcc tcatctgacc tgctggccta tggacggtgg
     5401 cagatacatc actctacctc tggtgatttc taaagatcct agttcaggaa aaagaaatgt
     5461 cggtacttac cgtatgcaaa tctatgattc tcacactgca ggcatgcatt ggcagtcaca
     5521 taaagtcgga gcaagccact acaggtccgg cgaactgaag aagttaccaa agatggaagt
     5581 tgccattgcg ttaggttccg accctgtaac tatgtggaca ggagcattac cgttacctcc
     5641 tgacatggac gaaatagcgg tatcaggggt attgagaaac tcatctgtcg aaatggttaa
     5701 atgtaaaaca gttgacctcg aagtacccgc ccattctgaa gccgtgctgg aaggatatat
     5761 aatccctggg gaattgaggg aagagggccc gttcggtgat cacaccggat actattctct
     5821 tgaagacaat tatcctgttt tccatctcac tgctttgaca cataaaagac atcccattta
     5881 tccggccact gtggtaggaa ggccaccatc cgaagatttc tttatgggaa aagctgtaga
     5941 gaggttgatg cttcctgcac tccaattaac tttgccagag gtagtagata ttaatatgcc
     6001 agcagaaggt gttttccata atttggttat cgtttcaatg aaaaaggaat atccaggaca
     6061 cacaagaaag gtcatgaatg caatttgggg attgggattg atgatgctgg caaaagggat
     6121 agtcattgta gacgacacag ttaatgtcca tgatttgtct gaagttgcgt ggcgagtcac
     6181 tagcaatatt aatccagata aagacattgt gatcaccgaa ggacctgtcg atgacttaga
     6241 tcatgcaagc ccaacaccta aatacgggag taaaatcggg atagatgcta caaaaaagac
     6301 cgcacttgat ggaagggaaa gaatttggcc tcctgacgta gtaatgtcag aggaaataaa
     6361 attgctggtc actaatcgct ggtcagagta cggtttttga tattcatgcc ccttaacagg
     6421 ttttggccga aaatacccaa tttctttgat gcaataaagt ttcaagaatc tcttttcgca
     6481 ctcccttttg cctacatagg aatgctgctt gcgattcggg gcctacctga ttttcaaagc
     6541 tttttttgga taacactcgc aatggtgtcg gctagaaccg tgggtatgat tgctaacaga
     6601 attatagaca gacacataga ctcaaaaaat ccacggtcgt cgcaaaggca tctcccacgt
     6661 ggaattcttt cagttaccga tttgttagtc cctggaataa tttcctcact catttttatg
     6721 ctatgcgctt acatgcttaa cactttagca ctgattttag cccctgtagc tctcatatac
     6781 cttgtggtgt acccgtttac caaaagggtc acttggttgg ctaatttgct tcttggatgg
     6841 gctctcgcca tagccccagc agcagcttgg atcggaatgc ttggtaaact aggatggcag
     6901 cctgttttac tttcaacttc agtggccctt tgggctggaa gttttgacat catctaccat
     6961 acacaagatg tttcttttca aaaaaaagag aagttacatt cagtcgcatc tcgttttgga
     7021 atctctaaag cctttaaaat tgcacagtgc atggatctat tggctgtatt gtgtctaata
     7081 atactaggcg catggatgca attaggaata ttttattacg gtggctgcgg attaagtaca
     7141 atttttcttg tctacaaata tatattagta aagcctagcg atttatctaa aatgggaatg
     7201 agcttcatga gaattaacgc ttttgtatct tgttacatgt tgttaggtac aattgcatca
     7261 ttaatagtgt aaaaatggaa aaactcgagg cacgaattgt ggttggtata tctggggcta
     7321 gtggatccct tttagccgcc aaaacggttg acgcattgtt agacagaaaa gttggggtat
     7381 cgctgatcgc gtcaaaccca gcccgaatgg tatggcagca cgagatgagt atgtcctacg
     7441 gagaaagtat tgaagaatgg tcatccaaca ataattttga gcatttcgct ataggggatt
     7501 ttaaggcgcc tatagcgagt ggaacttacc caactactgg aatggccata gtcccatcta
     7561 gcatggcaac aatttctgca gtggcaaaag gtctatcaga taatttaata agaagagctg
     7621 ccgacgtttg cctaaaagag aaaagacctt tagcaatggt tccacgagag tctccgctta
     7681 atgctataca tctaggtaac atgaaatacc tcgccacttt gggtgtgact atcctacctt
     7741 cagaccctcc atactacctg cctataacga cccttgagga agttgccaat tttacagccc
     7801 agagaatatt gatagcacta ggtataaccg accaactacc tgaggagatg caatatagag
     7861 gtccaattat ttaattctat tgtgctgatc taactggcaa ctataaaatg gtcatgatta
     7921 aaactgaaaa aggaagtgac atgatttcgg caatcgaggc ggtaaaggcc attagcgata
     7981 tcaggagtag tgaagtggtc gtagccacga tgaccgcaaa tagatattgg gaatctgtct
     8041 ctcaaaacca agaaatggat ttgcccatat ttggggcgat gggtaaagcc tcttcagttg
     8101 ctttaggtct ggcattatct caaccaaata aaaagatagt tgttttggat ggtgatggaa
     8161 gtttattgat gaatctaggc tccttggtaa ctgtggcagg tatggaacca aagaatttcc
     8221 ttcattttgt atttgaagac ggcgcatatt ggaccacggg aggtcaacct attccgggaa
     8281 acgggaaatt taatttatct gctatggcca atgatgctgg ctacaaaagc tcccattcct
     8341 ttgaagatct agaaacatta gtaagtgaac ttcccagtct gatgaggcaa gatggtccaa
     8401 tatttgtttc aattaaaatt actcatccag agcatgcagg tgagcctttt ataggctcca
     8461 caaaaaatgc gatggagaaa ctgtcaaaat caatatcagg ttattaatca atgtatgatt
     8521 ttcacaccca tactttttta agtgacggag tgctttcccc gatcgaactc ataaggcgag
     8581 ctattgcgat cgggtataag acgatggcaa taacggacca tgttggtcca ggcaatctgg
     8641 aatttgtgtt agctacctta aaaaaagatt gtgaaatggc agaaaaaaga tgggacataa
     8701 aagtttaccc aggtgtagaa atcacccata ccccaacaga agatatccac cttttggcca
     8761 aagaagcacg atccattggt gccaaaattg ttaacgtcca tggggaaacc ataatggagc
     8821 cggtaatccc tggaactaat atagctgctg tatcatccga gtatgtagat atattggcac
     8881 atcctggtct aataacggtc gaagaagcaa aaatggccgc caagaacgga gttttcttag
     8941 aaatttcagg tagaaaaggt catggtttgg ccaatgggca tgttgtgaaa acagctcgag
     9001 aaagtggtgc tacattgata ctggactcag acgcccatgc tccagaagac ttgatgactc
     9061 aggaatatgc tgaaaaagtg gctgctggat ccgggctaaa tgaaacagag cgtgccacat
     9121 tacttgagca aacaccttta agtctagtgt cccgagtcaa ttcgcgataa cctaatcgtc
     9181 tggaagaacg acagattctg gatctcctcc gttgacgact ttggcggtat tataggtggc
     9241 aaataccctg cttttgtctg aagcctcctg agtaaaagcg gctagatgtg gagtaatcag
     9301 gacattatcc atatcgatca gtggattatc ttcaggagtg ggctctactt caagcacatc
     9361 tacccccgca gccattattt tgttctgttc aatcgcacgg ataaaagcat cttcatcaac
     9421 cacagggccc ctacatgcat tgattaggat tgaagtgggt ttcatcaaat caaattctct
     9481 atcacttatc attcctctcg tattacgatt caaaggaaca tgtaaggtaa tgatgtcaga
     9541 tgtggataat aattcatcca atcccacctt ctccatagag agatcactca ccaattctga
     9601 atctggatca acgacatcat gatagataag ttcacactcc cagccttgta atctcttggc
     9661 tactcgagat cctattctgc caacgcctat tatgccgact gtttttccag ttaactcatg
     9721 tgcctgtgaa aaccagtcga ttcgaatgtt cgacatccat ttcccgtctt gcacactttt
     9781 aaattgcaac tgtaattttc ggtagactcc tatcatcaag gcgatggtat gctccgcaac
     9841 cgccacggcg ttacctcccc cgttattgga cactttgatt ccaagctctc caagtgcagc
     9901 cttatcaagt tggttggtac ctgcactagt agtttgaata aggcgcaatt ctgggcattt
     9961 agcagccaat tcaaggtcaa atacaagggc tcctcccaag attatcgcct tggtattttg
    10021 taatgcctca gtcgattcct ccaaggagcc cgaagagtta acccaatcta tttcaacccc
    10081 ttccggcatt gcatctttta acacttgtag accagaatca tccggggccc aaaaaacaac
    10141 ttgatcattc atattggtaa cataccccct aacggtaaga atactttctg tttgtgatta
    10201 tatgtagtta attacaacct ctcaactagg gagtcttttt gtttataatt ccacacgtag
    10261 aatacagaaa atattaggac aggctattga aaccccaagc tttgaaaaat gtgaggattc
    10321 tggatttgac tcactatata tctgggccat actgcacaaa actattgggt gattttgggg
    10381 ccgatgtcat aaaaattgaa aaccctctag tcggtgaccc ttgcagaaaa atgccaccct
    10441 ttgacgaaac caacccgaat agtaatgaca gtcttttatt tcaatatctc aacaccaaca
    10501 agaaaagcgt tgaactcgac ttaaaatccg atttcggtca tcttgctgct gaagaattag
    10561 tgaggaattc tgacatacta gtggagaatt ttcgacctgg aaccctggca tcctacggtt
    10621 taacagagag caaactgcgt accttaaacc ccaatttaat tgttgtatct attagtaatt
    10681 ttggccaaac gggaccttat agagactttg aggcaactga tctagtattt tatgctttgg
    10741 ggggattgat gttcattttt ggatcaatta acaaatctcc tctcaaacac gctttccacc
    10801 aagcacaatt caaagcaggc actaacgcag cctcagcagc attaatggct ttctacgaga
    10861 tgataaattc gggtaccggt cagaaagtgg acatatctat tcaggagagc atggctgcag
    10921 cggtacgtga tacaaccaca agttacgccg cctctggcat agttagaacg agacaaacat
    10981 taatgaacgg agagcttcct cgggcaccta tacaagtcaa agatggatat atcgtcccaa
    11041 tcacttttgg atctaccgac tggaacaaaa ctgccaatct gttgggagat gaaagtttgg
    11101 ctgaccacaa atttcagaca cctgaaggaa gaaggatgca tgctgaggaa tttgagaact
    11161 tggtgaaagc tgctttcatc cgtagaggta aacacgaact tttttacgga gcccactctc
    11221 accgtgaact ggtttacgga ttagtacaag acgcagcgga tctaatagaa aacccgcaat
    11281 atcaacatga tcaatatttt caggctattg gtaattccgg ttcagataca cccaaatttc
    11341 caggaagacc ttttaagcta tccaaaactc cttggaatat acaaacaact gcacctgcat
    11401 tagggcaaca taccgaggaa atactattta ataaattaaa tttacctatc gataaatttc
    11461 agaatactgg gcggtcaaat gaaaaataat ggcatcctca acggtatccg agtagttgag
    11521 ctaggccaac taatcgccgt gccttacgcc acaaaactcc tatcagacat gggtgcagaa
    11581 attatcaggt tggaatcagc tcaacggccg gatgcctacc ggcttgctga tttttacgat
    11641 gagagagagg atgggaaata ttggaatcga gccataaatt ttaatgagca aaatagaaac
    11701 aaactaagtc ttggactaga attagaccat gaaagcagcc ttgaatattt gcacgatttg
    11761 attagcataa gtgatgtctt tgcatgcaat ttcaccccta gagtgatgaa aaaattcggc
    11821 ctagagtacg agcatctcca aaaaatacgt cccgatataa tcgtcatttc atcaacgggt
    11881 tatggtcaca ctggaccgtg gtcgagtttt ggtgccatag ggtttggtac tgaggctgcg
    11941 tcaggacttg cggccgcaac aggatacgaa aatggcccac cagttcaacc agaaatacct
    12001 tatccagatt acactgccgc cgagcacact gtctttgctg taatggcagc gcttatacat
    12061 agactaaagg aaggcgaagg ccagtttatt gagatatcgc aagcagaatg tgttacatca
    12121 actatcccgg aaacaatact tgaatacaca gccaacaaaa agatagttaa gcgaatgggc
    12181 aactcgcatc ccaacatatc tccacatggc tgttatccat gcaaaggtca agataaatgg
    12241 ataaccatca ctgctaattc tcaggatcaa tggaaatcat tatcaaattt gttgcttcca
    12301 gaaactggca ctgacaatcg atttgaatct aatgagtcac gcatacataa ccgaaaagaa
    12361 ttagacaaat tgatatcttc aagaactcta tcatgggaca atgtcgtatt gatgaatact
    12421 ttacaaagaa aaggtgtggc tgcaggggca gtattgaatg gtaaagaact gctatttaat
    12481 gagcatttga accaaaggaa tttttttgaa atagttgatc atcatccaga aagtgaagtt
    12541 cccacacttc catatcctgg caagccatgg aaaatgtcgg caaattcaga tgtcaaaatg
    12601 aagggagcac ctttatttgg acagcataac gaatcaatat tgaaaaatca cctgggattg
    12661 accaacgaat cctatgatgc actgattaat gaagaagcag tggttaacta tattaaatta
    12721 gaccccaaag aaattcttgt atcaagagag gcgagagaag aagaagggtc tatatattct
    12781 tatgatcctg atttcaaatc caccataaaa tcttatttca acatttgaaa tcacagatat
    12841 taaggcaaaa atttatttta tgaaatttca aatagcaata gatggccctg ttgcctctgg
    12901 gaaaaccgct gtgggaagag gtgtcagtaa ggctttgaaa tggaatttcc tagacacagg
    12961 gataatgtac agagctgcaa ctagatctat attggattca ggcatatcat ttgaggagaa
    13021 ttcaagcatc attgaaaccg tcaagaaaac gacccttgac attgacaact ctccaggttt
    13081 tgaaaaaata ctgataaacg gaaaagataa aacctccaca cttaaaaccc ctgagataga
    13141 tcgagcagta tcaatcattt caaagatccc tgaagtaaga aaagaactgg taaggcagca
    13201 acgaggtatt gccagccaag ggccaatcgt gatggtagga agagatatag gaagtgtcgt
    13261 gttacctgaa gcagaattaa gaatatactt agaggccaat gttgaaatta gagcccagcg
    13321 acgctaccaa gaacaaattg catccaaggg caaattatca ttgaacgatg taaaagttga
    13381 tctggagaaa agagacctca tagacacaca gcggcaagat tcacccctga gtaagtatga
    13441 tttctatacc atcattgata ccacaactat gaatttggaa gatgtggtgg atgaaataac
    13501 atctatttac agatctcaaa tttaatgctc tacccaacct gtaattttct acaaaagatc
    13561 actttaaaat gctttgccaa atttgaagtt tctggcaagg aaaatgttcc accagtagga
    13621 cctctaatcg tagtttctaa tcacttaagc aacatagatc catcaatttt ggcggtttcg
    13681 gtttctagaa gattgcaatt tcttgcaaaa agcagcttgt ttaccggacc tttcctcagc
    13741 aaacttctac attggtatgg tgcttatcct ctaaatagag acaaaattga tgtaacggcc
    13801 ttcaggtgga ccctaaatca gttacgccag gacggcgcca ttttaatttt ccctgaaggg
    13861 acgagaagta aaggatccat gattaaagcc aaaccgggag tagctagact agtccaaatg
    13921 tccgacgcga ctatattgcc agtagcgata actggaactg aacacatcgg taatgtagta
    13981 agagtggtga acccaacagg aaatataaaa gtaaatattg gaagcccgtt
//
LOCUS       DASCNS010000152        26072 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001194007, whole genome shotgun sequence.
ACCESSION   DASCNS010000152 DASCNS010000000
VERSION     DASCNS010000152.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 26072)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26072)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26072
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001194007"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(95..709)
                     /locus_tag="QF838_05490"
     CDS             complement(95..709)
                     /locus_tag="QF838_05490"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF011325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HJO60170.1"
                     /translation="MNHKDVQPPILLILSGPSGVGKDAVLSRMRDLEEPYHFTVTTTT
                     RTKRNGETDGKDYIFVSQAEFRAQMEQDGFLEWAEVYGNYYGVPRNQVKTALKKGKDV
                     IVKIDVQGAKTIKSLAPNALYIFLAPPSMDQLEKRLKERMTESPDSLKIRFETAAEEM
                     KSAGWFDHVVINHENQLDLAVAEIQNVVKIARLKQMETPNFEII"
     gene            complement(723..2027)
                     /gene="aroA"
                     /locus_tag="QF838_05495"
     CDS             complement(723..2027)
                     /gene="aroA"
                     /locus_tag="QF838_05495"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HJO60171.1"
                     /translation="MISKITSPDRLHGIVKPPGDKSISHRAGLLNSISEGVSHVSNFC
                     VGDDRSSMLGCLKGLGANITRHTNCKISGAEECFEIIGSGLNGLAEPQDILNAGNSGT
                     TMRLISGLLAGQDFFSVITGDASLRNRPMKRITTPLTQMGAYINGRDDGSLAPLSFTG
                     SSLKGIEYTMPVASAQLKSCLIIAGLYSNSPSIIHQPAESRDHTERMLNSMGGDLEIN
                     GLTIKTGNKELSSVDVKVPSDTSGSAFWLIAGVCHSDSRITIPGMGMNPTRTGVLEVL
                     ESMNANITIENKHLEGGEPAGDVTASTSDLVATEISGDLIPRVVDELPILALAACFAR
                     GTTVIANAEELRVKESDRISATVDSLQRLGAQIEETSDGMRITGTHTLTGAEMETHGD
                     HRIAMTNGIAGLLASGETIIGNSEDASVSYPSFWEVVSELQK"
     gene            complement(2036..3037)
                     /locus_tag="QF838_05500"
     CDS             complement(2036..3037)
                     /locus_tag="QF838_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014234.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HJO60172.1"
                     /translation="MERITVVGMGALGASLGLALKNIRLKNTEVIGHCLDRKAGSEIN
                     KIEAFDALEGNLKKAIEGAQLIVLDYPINESRDLFESIGSFVSDGAVVTDMRSSKKRS
                     AAWAEEFLPGSVSYVGGRPIIKTPTKGIGDSSPNLFDGVNYCVIPSKSADDQATKTVV
                     GLAESIGAKPYFLDVDEHDSYSAAMDHLPYLISTAFVNATSSNASWREMHKSAGSLFD
                     MQSTLSANDPTDTEADSLTMSEPLIYWVDQMILSLHKLRTELQEDSESFIESFIHAWE
                     QRARWEAGVVGQNSDMEELPSAAESMASAFLGDKLARRVTKMGDANKKESWRYPRSR"
     gene            complement(3156..3572)
                     /locus_tag="QF838_05505"
     CDS             complement(3156..3572)
                     /locus_tag="QF838_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJO60173.1"
                     /translation="MPVDIYRIKKAPPLLGMSMVSFAGPASNLVTAILIGFCLKKLAL
                     EFGLSEIWIELGFTFVQISLILAVFNLVPLPPLDGYKVILGILPRNLSILYSGIEKYG
                     PFALIILFFIDMTVPGISILSTLIGGPVKILLNWIF"
     gene            complement(3871..4305)
                     /locus_tag="QF838_05510"
     CDS             complement(3871..4305)
                     /locus_tag="QF838_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HJO60174.1"
                     /translation="MATTSLIQVSKLAADKLSEILKEQGEENGMLRVMVTPSPNGGFQ
                     HVLGVESEPKDEDIVIEAFGIKVVIDGESAPLLEGAEIDYVDGLMRSGFVISNPNIEH
                     SGGGCGCGGGGGGCGAGGGGCNCGGGGCGGGGCGGGCGCGGH"
     gene            4580..4696
                     /gene="rrf"
                     /locus_tag="QF838_05515"
     rRNA            4580..4696
                     /gene="rrf"
                     /locus_tag="QF838_05515"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            4867..6255
                     /locus_tag="QF838_05520"
     CDS             4867..6255
                     /locus_tag="QF838_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012784.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HJO60175.1"
                     /translation="MPPETQDNSPQDMQQLLEEMDFKILRRGEVVEGSVMRVDSDGVF
                     LDIGHKEEGFVPLGEMKTISKEEMVKLSEGDSLIAFVMRPDSKDGPILSVDRARGEEG
                     WRDIQKLMDQDEPVEGVIAGFNRGGCILEVANVQGFVPMSQLITISRDLFKQESSEPG
                     GANVSEDTIGKDLIGKTLTVKVLEVNRSRNRAIFSERSALQEQRDEQKAELIQKLEEG
                     EIRKGIVTGISNFGAFVDLGGADGLIHISEMSWSMVGSPEDLVKVGEELDVYILRIDR
                     ETMKIALSLRRLQPEPWETIFERYQVGDVVDAIVTKIANFGAFARLEDSIEGLIHITE
                     LSDAVITNPNEVVSEGQEIKVKILKIEMERKRLGLSLKQTSETLPPEGYISEAPVEDQ
                     EETKGDDGKEDSTLSSDVTEPDAAEATEPDAADVTEPDAADVTEPDAAETNNSEEEKD
                     DVVEPTQDTEKE"
     gene            6276..8753
                     /locus_tag="QF838_05525"
     CDS             6276..8753
                     /locus_tag="QF838_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HJO60176.1"
                     /translation="MASRFEKFSERARRVLSLAQEEAQRFNHNYIGTEHILLGLVREN
                     EGTAAKVLGGLGIELNKIRSAVEFIIGKGDTAASGEIGLTPRAKKVIELAVDEARRLN
                     HHYIGTEHLLIGLMREGEGVAAGVLESLGVTLDKVRGETNSVLNKGARSSKGRSGGKT
                     TTKTPTLDQLGVDLTEKANDGKLDPTVGREKEIERVTQILSRRTKNNPVLIGEPGVGK
                     TAIVEALAQRIASDDVPGTLQGKRLVTLDMGSLVAGTKYRGEFEERLKKVIEEIKSAG
                     NCVLFIDEIHTMVGAGAAEGAVDAANILKPSLARGEMQTIGATTLDDYRKYVERDPAL
                     ERRFQPVRVEEPSVDETVDILRGVKEKYEEHHQLEISDSAIQSAASLASRFIADRFLP
                     DKAIDLIDEASSRVRIHHSMAPRSVKEASQMLDSVRKEKDDAISDREYEHAAELRDRE
                     VSLSEKLENIKKEWEEEKGDQQPIVEEENIEEVVSMWTGIPVTRLAAAESERLIKMEE
                     ALHQSVIGQQEAIEIMSKAVRRSRSGLKNPRRPTGAFIFLGPTGVGKTYIVKKLAEFM
                     FGSEDSMIRLDMSEFMERHTVARLVGAPPGYIGYDEGGQLTEAVRRKSYSVILLDEIE
                     KAHPEVFNILLQIFDDGHLTDSKGRKVDFRNTIIVMTSNIGSDLIRQDRSLGFNARTE
                     GAQDEEESYLRMKNNVLDEVKHFFRPEFLNRIDGTVVFHALSRSHMEDIVNILMKEVS
                     SNLLEKGISLEVTDAAREWLAEEGYDPTFGARPLRRLIQDTVEDKLSDALLAGEIGPA
                     DTAIVDLDENDLIIKTETPASLTSSSV"
     gene            8792..9979
                     /gene="larC"
                     /locus_tag="QF838_05530"
     CDS             8792..9979
                     /gene="larC"
                     /locus_tag="QF838_05530"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HJO60177.1"
                     /translation="MKAAYVNAIGGASGDMLLASLIDSGLDLVELSSALASVGVEGFE
                     LEALSGDRRGVKGTHLDVNIDRADPGKRTPEDFIDIVNTAPISESSRSKCREVFNLIG
                     EAEGSVHGEDKGKVHLHELGTIDTIIDVVGVVLGLEMLSVEKLYSSPIPTGSGTVRTE
                     HGLLPVPAPATAYMLTSKDIPCYPPPSYMPPTGEMVTPTGMALLATLADFSQPVMNIH
                     SIGYGLGTRNPESYPNVLSLWLGDLSTPTDETRIILLETNLDDTTGETLGYVQEKLFE
                     LGARDVWVTPIQMKKNRPGVLLSTLIDESMKEKISDFIMLETSTLGIRVRPVERIEAE
                     REIENFESSLGNVRIKLKKKNGLVVAVAPEYEDCKKIASEDSIPLNQVFFLMKREAED
                     IYL"
     gene            10150..10237
                     /locus_tag="QF838_05535"
     tRNA            10150..10237
                     /locus_tag="QF838_05535"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10184..10186,aa:Tyr,seq:gta)
     gene            10271..10345
                     /locus_tag="QF838_05540"
     tRNA            10271..10345
                     /locus_tag="QF838_05540"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10303..10305,aa:Thr,seq:ggt)
     gene            10366..11565
                     /gene="tuf"
                     /locus_tag="QF838_05545"
     CDS             10366..11565
                     /gene="tuf"
                     /locus_tag="QF838_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HJO60178.1"
                     /translation="MAKQVFDRTKPHLNVGTIGHVDHGKTTLTAAISSVLASEGLADF
                     RAFDTIDNAPEEKERGVTIAITHVEYETDTRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLARQVEVPRMVVALNKVDMMDDEELLELVELEMRELLSE
                     YGFPGDDIPFIRLSALNALEAEDKGKDSEWGKGVWELMDAVDEYIPVPDRPKDQPFLM
                     PVEDVFGIKGRGTVVTGRVEKGIVNVGQEIEVVGIKDTYTTIVTGVEMFHKTLDSGEP
                     GDAVGVLLRGVDREDIERGQVLCDPGSIEPHTEYEAQVYVLSKDEGGRHTPFFNGYKP
                     QFYIRTTDVTGEIQLPDGVEMIMPGDNVEMKVKLITPVALDEQLRFAVREGGRTVGSG
                     VITKVLE"
     gene            11645..11812
                     /gene="rpmG"
                     /locus_tag="QF838_05550"
     CDS             11645..11812
                     /gene="rpmG"
                     /locus_tag="QF838_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HJO60179.1"
                     /translation="MARRGENRILVTFGCTDCRQRTYNSNKSRRNDPQRMELKKYCPR
                     CRAHTLHREVR"
     gene            11814..12026
                     /gene="secE"
                     /locus_tag="QF838_05555"
     CDS             11814..12026
                     /gene="secE"
                     /locus_tag="QF838_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HJO60180.1"
                     /translation="MQQRYGRTSTRRSINFRFFGEVIGELRRVTWPTTNETFRLTLMV
                     LAVAAVLGVVLGVADLVFGWVFDTIL"
     gene            12047..12607
                     /gene="nusG"
                     /locus_tag="QF838_05560"
     CDS             12047..12607
                     /gene="nusG"
                     /locus_tag="QF838_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HJO60181.1"
                     /translation="MIEEQTENRRWYIIHAYSGQEDRVKKNLEQRIQTMDVKDKIFQV
                     VVPTEEEMEYKDGQKKTNLKKIFPGYILVQMEMDDDSWFVVRNTPGVTGFVSAEDESD
                     NRPKPVPLEQGEVDHILNQIRSDEPRIKVGFASGQTIRVTEGPFIDFMGVVDEVYPDR
                     LKVKVLVSFFGRETSVELDFLQVEKT"
     gene            12625..13047
                     /gene="rplK"
                     /locus_tag="QF838_05565"
     CDS             12625..13047
                     /gene="rplK"
                     /locus_tag="QF838_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HJO60182.1"
                     /translation="MAKKVIGLIKLQIEAGKATPAPPVGPALGQHGVNIMGFVKEYNE
                     KTASMAGAIVPVEITVFEDRSFNFIVKSPPASDLLRKAAGIPKGSGSPLAEKVATIPR
                     SKVKEIAETKIKDLNAADIEGAMKIIEGTARSMGIVVE"
     gene            13088..13801
                     /gene="rplA"
                     /locus_tag="QF838_05570"
     CDS             13088..13801
                     /gene="rplA"
                     /locus_tag="QF838_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HJO60183.1"
                     /translation="MAKRGKRYDTAIAEIDPESPYDPKEAVSIAKRNATAKFDETMEL
                     HLRTGADPRHAEQMVRGIALLPHGVGKEIRTLVFCQGDAIESAKQAGADYVGNDDMIA
                     EIEKGWLDFDVSIATPDMMGKISKLGRILGRRGLMPNPRTGTVVSAEDIPRAIEEAKK
                     GRVEFRLDKSALIHAPIGKASFDEDQLMENLTTLVDTILRGRPSGVKGQFLRSAFLTS
                     TMGPSVPIDIAGIMSLRVE"
     gene            13955..14506
                     /gene="rplJ"
                     /locus_tag="QF838_05575"
     CDS             13955..14506
                     /gene="rplJ"
                     /locus_tag="QF838_05575"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HJO60184.1"
                     /translation="MPTNRKALDVDSLMEAMRGCTIAISTDYSGLNVSEMSEFRTTLR
                     ENGIKYKVVKNTLFKIAADQAEMPAIKDLIDGTTAIAFGYGDEQIPAKVITKYVRDSG
                     VELKIRNALIGDTTLSSEQLKELAALPGKEELAAKLVGQLYGQLAGLVHVMNNPLLRL
                     AKSLNSPSSGLVNVLNSRVRQIS"
     gene            14548..14940
                     /gene="rplL"
                     /locus_tag="QF838_05580"
     CDS             14548..14940
                     /gene="rplL"
                     /locus_tag="QF838_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HJO60185.1"
                     /translation="MALSKDEILEAIKELPVIELAGLVKDLEEEFGVSAAAAAPVAVA
                     AAPAAAGDSAGAAEEEQDAFTINLKEIGENKINVIKAVRAATSLGLKEAKDLVESAPV
                     AVKEGVGKEEADNVKKELEEAGAVVELI"
     gene            complement(14966..16282)
                     /gene="larA"
                     /locus_tag="QF838_05585"
     CDS             complement(14966..16282)
                     /gene="larA"
                     /locus_tag="QF838_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HJO60186.1"
                     /translation="MKIDLAYGKTGLTIDLPDDRTSVIEPQFIPGLPDELGAIKTALR
                     NPIGTAPLRKSVAPGQNIGISVCDITRPMPNKRVLPIILNELKHILPSNITIFIATGT
                     HRKNTHSELISMLGEQPMSGGYNIVNHDAFNRDNLTEVGVTKKNIPVFLNSDWIKTDF
                     KITTGFVEPHFFAGFSGGPKMIAPGLAGFETIMKLHDSDMIGNTKSKWGITYGNPIHD
                     SIREIATLSPSDFTLDVTINKNHKITSVYGGEMFQVHKAACEFAHSTAMREVSEPFDI
                     VVTTNSGYPLDMNLYQAVKGMSAAHQIVKKGGSIICAAECSDGIPNHGSYGEILKSQT
                     SPQSLINMIESSDYQKHDQWQVQIQASIQMENDVYIKSDNLSIEQIVEAHLLPTEDVS
                     MKVRELLSKKYQKGTVCVLPEGPQTIPFLSGVDRDGQCRKVRPTNC"
     gene            complement(16301..17380)
                     /locus_tag="QF838_05590"
     CDS             complement(16301..17380)
                     /locus_tag="QF838_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013696.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HJO60187.1"
                     /translation="MIRRERLVQTFCDLARIDSPSGEEEDMALNISSRLETLGFKTMR
                     DAYGNLIASDERPDPILLSAHLDTVEPGRGIKPSVKGDRIVSDGSTILGGDCKAGVSA
                     ILEALESIMEDGVEYHPVELAFTREEEIGLVGARNLDFNLITAKEAIVFDGEGPVSQI
                     TASSPTYVGFDIEITGRAAHAGVEPEKGLSSIKIASEIITRLPQGRLDENTTVNVGTI
                     EGGTVRNAVPETTTIRGEFRSTSIENLDGLRVQITDVVKEVEKLFPEATIDNHLHVEF
                     ETYTLTDDDPATMRVKETLKGLGLDPSMKPSGGGTDGNVFRLNGISSVVVGMADHGMH
                     TVREYVTIPDLVDASHLCEALLRTK"
     gene            complement(17439..19139)
                     /locus_tag="QF838_05595"
     CDS             complement(17439..19139)
                     /locus_tag="QF838_05595"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HJO60188.1"
                     /translation="MATLRLALAQINPTVGDLSHNSDVIIHHIEKAEQEGIDIIAFPE
                     LAVTGYPPEDLLLKHNFIKDNMDAAERIARSTGEITAIFGYAERVEDKIYNSAAIVSN
                     HEILDSYQKMYLPNYGVFDEKRYFTEGSDFPVYDLDHVRFGVNVCEDIWFSPGPSDIQ
                     SDNGASLIININGSPFNLGKRSQREKVLIERAKSNGVFIAYINMVGGQDELVFDGGSL
                     IVDPAGNILMRANQFEENIYIYEIDLPTYKPSTTQDSVNEIKKVILPGIIKESSSNIP
                     LTPLTIIDQNEEIYRALVLGTRDYVRKCGFTKALIALSGGIDSSLVAAIAVEALGSEN
                     VKGISLPSKFSSSGSKVDAKKLADNLEIPMDTIHIEEILQSMEKGLNDQFQGTEWGVA
                     EENLQSRIRGNIMMALSNKFGFLVLTTGNKSEMAVGYATIYGDMAGGFSVIKDLPKGM
                     VYEICHYINMKHGYDLIPNSILTKPPSAELKPNQKDSDTLPDYDILDAILEKYVEEDL
                     GIADILNIGFDPGTVAEILKLVDINEYKRRQSAPGIKITSRNFGRDRRMPISNKYRPY
                     "
     gene            complement(19210..20376)
                     /locus_tag="QF838_05600"
     CDS             complement(19210..20376)
                     /locus_tag="QF838_05600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HJO60189.1"
                     /translation="MKLAQRMSRLGTETAFEVLAKAQQLEAKGRNIIHLEIGEPDFDT
                     PTNIVEAGRQALSDGFTSYNPSPGYADLKESIAKDISKSRNIRVKKENVIVTPGGKPI
                     MFFIILAMIENGDEVLYPNPGFPIYESMIEFCGGTAVPVRLGEENSFNLDIKELESQI
                     SEKTKLLIVNSPNNPCGSVIDKKDLEALSKLAKENDITVLSDEIYSRFLYEGTHHSIA
                     SFPDMIDRTIILDGYSKTYAMTGWRIGYGVFPDDLVEPISRLVTNSVSCTASFTQRAA
                     IEATEGPQEDSILMVEEFKKRRSIVVDGLNKIDGITCALPKGAFYAFPNVEGTGMTSS
                     EFANRVLEEAGVAVLAGESFGEYGTGYIRISFANSTENLIEAIDRIRNFVESKT"
     gene            20527..21441
                     /gene="ispH"
                     /locus_tag="QF838_05605"
     CDS             20527..21441
                     /gene="ispH"
                     /locus_tag="QF838_05605"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HJO60190.1"
                     /translation="MNVYLGVPRGFCAGVVRAIDIVELALKKYGPPIYVKHQIVHNPH
                     VVKDLESKGAITVENVNEIPRGSKVVFSAHGSPPEDFKNAKEQGLQVLDATCPLVIKV
                     HNEAKKYNRDGRKIILVGHKGHQEVKGTTGQTDMKIVDDREEFVPPKWDNSIPVAVLT
                     QTTLSVDDTENSIRNIQNSFTDVLVRNDLCYATTNRQMAVKEISNHVEVFLVVGGQNS
                     SNCNRLKEVAESNGVPAFLINNVEELDPKWLAGVVNVGITSGASTPDSLVRGIIDFID
                     PDNVIEMGGEEENITFTLPKELRDIGDA"
     gene            21443..22150
                     /locus_tag="QF838_05610"
     CDS             21443..22150
                     /locus_tag="QF838_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015962.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HJO60191.1"
                     /translation="MEIRIESLIAGAREAEGVVVVVDVFRCFTTEAVAFANGAKEIIL
                     VAEIEEAFELKRKGMGDVLMGEVGGKRPEGFDFGNSPFELKDVDLTDKTIIQSTRAGT
                     VGVTAASKADVIYGGSFAVASATATNILSAQPEIVTIVAMGLEGKIRADEDEQCALYL
                     RNTMQGRTPDHKAVRSLVISGEESQKYETAEYPQWPIEDREMALDINSHSFALMINKE
                     DGYFVSRPENIVNNGRL"
     gene            22150..23076
                     /locus_tag="QF838_05615"
     CDS             22150..23076
                     /locus_tag="QF838_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO60192.1"
                     /translation="MKYRKLGKSGLEVSVVGLGANNFGGRLDYESSNSVISQLFDVGI
                     NLIDTSNSYGNTMSEEYIGRSLEGRREKAVIATKVSSRMSEGPNQAGNSRKHIIEQVE
                     NSLARLRTDYIDLYQIHWWDPNTPIEETLRVLDDLVTQGKVRYFGCSNFSAWQVCESI
                     WTSRELGIQTFISAQPHYSMLERNVEQELIPFCGKYGVGILPYFPLANGFLTGKYRKN
                     SPPPEGSRLEADNKGLFTEDNYALLEKLINFSEERGKTVLDLAFAWLLYNNDISSVIA
                     GATKPEQVISNADAADWQLTDKEYEEVTQFLP"
     gene            complement(23078..23623)
                     /locus_tag="QF838_05620"
     CDS             complement(23078..23623)
                     /locus_tag="QF838_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014664.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HJO60193.1"
                     /translation="MARIKQITSKNEVSDQHHEIFDSIASSRGRISGPFSVLLHSPEV
                     AGRAAHLGAYIRFESLLKDDEREVAIITAAREMNCNYEWAAHVPIAMEAGVTQEVVNV
                     INKRTSTNKVAPNYSLVIRYARQLINDKKVDQETFDQAVSKYGEQGVTELTAAIGYYG
                     MLACALNVFEVTPDSGKPTLI"
     gene            complement(23625..25532)
                     /locus_tag="QF838_05625"
     CDS             complement(23625..25532)
                     /locus_tag="QF838_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60194.1"
                     /translation="MIWVRRIIALPFIIMAFVTFQVGVLAQQTASNLINPSFYLETLA
                     ESNIYQFLLTDLPRTALKDVRKANSNPIIDQSGLSDEIIITSINEIIPPEWLQSNFES
                     TVTGVGDYVTGKSDDFTISIPVDERVQAASNQITFILNESDLYKLVMENQVRPVVSQA
                     SKNELPFDVSVSEDQLMGSIQKIISKSWLTGQIDSVLGEVVPYAVGAKDTFAVVITVD
                     DRIEVAVAEVKFLLAEANAYESLFEGSIAPNISSSIGNTAKLPYGVEITDEEISAIIK
                     KTAPAAWVQKTTESILDNATPYLVGRTDEFTISIDIEPNKEDAVSDLMALAEQKLNSK
                     LDNLPDCDAAEVANILSNPVGGIPSCYPADPALKGQMQSYTKAYISTVISAVRPQIIN
                     TIPNLIEFDQDSLRQVVPPKALDSFDQGRAIMREGYTFRETDLESLIKQGAGDNSWDQ
                     VNTVRNSLSQGIQYNDQDFRVHIETITADGGQTLSMLDQLRGILKLVHMFNLAVYIPT
                     ILIAALVGFLGGRGWNQRLMWAAIAMLIASFLVYVIWGPIYSSFAEPIIHVQIDQIAS
                     QTSGQIAPQFLATESLVLQQVTSIGKIAISKFIAGISGTALITSIISVVIIAGCVILK
                     KINFKEQFRGK"
     gene            complement(25637..25855)
                     /locus_tag="QF838_05630"
     CDS             complement(25637..25855)
                     /locus_tag="QF838_05630"
                     /inference="COORDINATES: protein motif:HMM:NF041211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 2"
                     /protein_id="HJO60195.1"
                     /translation="MSDTIVLYTSPDCTYSDALKEELIDLALDFEEINLALNPELWEK
                     VEELTGGERITPVMVTGDNVEIGFHGVG"
BASE COUNT         7504 a         5130 c         5858 g         7580 t
ORIGIN      
        1 gcatccgtga gtttcagcat ggatactcaa aatgatgatg aagaaaatat cttacgagac
       61 gttataaaaa accttaaaga ttcctaaatt tcattcagat aatttcaaaa ttcggcgttt
      121 ccatttgctt tagccgtgca attttaacca cattttgaat ttctgcgaca gccagatcca
      181 attggttctc atggttgata acgacgtgat cgaaccaacc tgcggatttc atttcctcgg
      241 cggcagtttc gaatctgatc ttaagactat cgggagattc agtcatccgt tctttcaatc
      301 ttttttctag ctgatccata ctaggaggcg ctagaaatat atataaggcg tttggggcca
      361 aacttttaat agttttggcc ccttggacat ctattttcac aatgacgtct ttgccttttt
      421 ttaaagctgt ttttacttga ttacgtggta caccataata attaccgtaa acctctgccc
      481 attctagaaa cccatcttgc tccatttgtg ctctgaactc agcttgactg acgaatatat
      541 agtctttccc atctgtttca ccatttcttt tggttcttgt agtggttgta acagtaaaat
      601 ggtatggctc ctctaggtct ctcatccgag aaagtactgc gtcctttccc acgcctgacg
      661 gtccagaaag tattagtagg atgggaggtt gtacatcttt gtgattcaaa agattagtaa
      721 cctcactttt gtaattctga aacgacttcc caaaaagaag ggtaggagac actggcgtcc
      781 tcagaatttc cgataatggt ttcccctgag gctaataagc ctgcgattcc gttggtcatg
      841 gcaatcctgt ggtctccgtg ggtttccatt tcagcaccag ttagcgtatg agttcccgtt
      901 attcgcattc cgtccgatgt ttcttctatt tgtgcaccta atctttgtag actgtcaaca
      961 gttgctgaaa tccggtctga ttctttaact cttaattctt ctgcattagc tattacggtt
     1021 gtgcctctag cgaaacaggc ggcgagagct aatataggta attcatcaac gacccgaggt
     1081 atcagatccc ctgatatttc agttgctact aaatcactag ttgatgctgt tacatcacct
     1141 gccggttctc caccttctag gtgtttattt tctattgtta tattcgcgtt catggattca
     1201 agtacttcca agaccccggt gcgggtaggg ttcataccca ttcccgggat cgttatcctt
     1261 gaatcagaat ggcacacacc ggcgatcaac caaaaagcag atccactagt gtcgctaggt
     1321 accttaacgt caacagaact gagttctttg ttccctgttt taattgtgag gccatttatt
     1381 tccaaatccc cgcccataga gttgagcatc ctttctgtgt gatcccttga ctctgcaggt
     1441 tgatgtatga tgctggggct atttgagtat aatccggcaa ttatgagaca ggattttaat
     1501 tgggcacttg cgaccggcat cgtatactct attcctttta aagaactacc tgtaaaagaa
     1561 agtggcgcta gagaaccatc atcacgacca tttatatagg cacccatttg agttagcggt
     1621 gtggttatcc ttttcatggg ccgattccta agagaagcgt ctcctgttat cacgctgaaa
     1681 aagtcttgcc ctgccaacaa accggatatt aaccgcatgg tggtaccgct gttaccagca
     1741 tttaaaatat cttgtggttc ggctagacca ttaagtccag atccgattat ctcaaagcat
     1801 tcttcggctc cggaaatttt acaatttgta tgtctggtga tgttggctcc taatccttta
     1861 agacaaccta gcatggagga tctgtcatca ccgacacaaa aattagatac atgtgataca
     1921 ccttcagaga tgctattcaa taaacctgct cggtgtgata ttgatttgtc ccctgggggt
     1981 ttcactatgc catgcaatct atctggggat gttattttac tgatcatatt gctttctatc
     2041 tgctcctagg atatctccag gactcttttt tatttgcatc acccattttt gtaactcttc
     2101 tcgcaagctt gtccccaagg aaggcagaag ccatagactc agcagcactt ggcagttcct
     2161 ccatatctga attttgaccc actacgccgg cttcccatct ggctctttgt tcccaagcgt
     2221 gtataaaaga ttctatgaag ctttcactgt cttcttgcaa ttcggtcctt agtttatgta
     2281 gcgataggat catttgatca acccagtaaa tcaatggttc tgacatagtc aacgagtcgg
     2341 cctctgtatc tgttggatca ttagcgctta atgtggactg catatcaaat aaacttccgg
     2401 cacttttatg catctctctc caagaagcat tactggaagt ggcatttaca aaagcagtgg
     2461 aaatcaaata aggcagatga tccatagcag cagaataact atcatgttca tcaacatcta
     2521 agaaataggg ttttgctcct attgattctg ctaatccgac cactgtttta gtggcttggt
     2581 catctgcgga ttttgatggg attacacaat aattgacccc gtcaaacaaa ttaggtgaag
     2641 aatcgcctat ccctttagtt ggggttttaa ttattggccg acctccgaca taagagacgg
     2701 agcccggtaa aaattcctca gcccaagctg cagacctctt ttttgatgac cgcatatctg
     2761 tcacaactgc accatctgac acaaaagatc ctattgattc aaaaagatcc ctagattcat
     2821 ttataggata atcaagaaca ataagctgtg ctccctcaat agcttttttg agattacctt
     2881 ccagtgcatc aaatgcttcg atcttattaa tttcagaccc tgcttttcgg tctaggcaat
     2941 ggcctattac ttcagtgttt ttgagcctga tattttttaa agctagtccg agtgatgctc
     3001 ccagtgcgcc cattccaacc acagtgatac gttccaaagc tacacctctt catggtgtcc
     3061 aagcttcaat ttgtgttgaa gccggatgtt ttatttatga tttattataa agaattaaaa
     3121 cttatgaaaa tgatacacga cgacataaat aggtattaaa aaatccagtt caatagtatt
     3181 ttcactggcc ctcctattag ggttgataaa atgcttatac cgggtacagt catatcgata
     3241 aaaaacagaa ttattagtgc aaatggccca tatttttcaa tacccgaata cagtattgat
     3301 aggtttctag gtaatatgcc taaaattacc ttgtacccgt ccaatggtgg cagagggaca
     3361 aggttaaaaa cagctaggat taggctaatt tgtacgaaag tgaatcctaa ttcaatccat
     3421 atttcactta acccaaattc taaagccagt ttttttaaac agaaccctat taaaatagct
     3481 gttactaaat tagaggctgg gccagcgaaa gataccatcg acatgcctaa taaaggaggc
     3541 gcttttttga ttctgtatat gtcaaccggc actggtttgc cccatccaaa tccgacaaat
     3601 aaaaataaca aggagccgag tgggtcaagg tgacttttag gatttagtgt caatctgcct
     3661 aatgatttgg ctgtggtatc acctagtttg ctcgagacca atgcatgtga aaattcatgt
     3721 attgtgactg cgactaaaat ggaaagtaat actagactca aagatacgag ggttgagaaa
     3781 gggtctttta aaaatgaatc taagtattgg aggatcattt cttaagtgaa aaggggcggt
     3841 ctacaccgcc cctttttaaa tttctaaatc ttagtggcca ccacaaccac aaccaccacc
     3901 acaacctcca ccaccgcaac caccgccacc gcaattgcag cctccgcctc ctgcgccaca
     3961 acctcctcct ccgcctccac atccgcatcc accaccagaa tgctcaatgt tcgggtttga
     4021 gattacaaac ccacttctca ttaaaccgtc aacataatca atttcagctc cctctagtag
     4081 aggtgcactc tctccatcga taacaacctt gatgccgaat gcctcgatga ctatatcttc
     4141 atcttttggt tcagactcca ctccaagaac atgctggaat cctccattag gactaggagt
     4201 aaccataact ctgagcattc cgttttcttc accttgttct ttcagaatct cagaaagctt
     4261 atcggcagct aacttagata cctgtattag gcttgttgtt gccacttttt caactccttc
     4321 actcgcccgc ttctttaagt tatacccttc gcaattgcaa ataattatcc aactacctac
     4381 ggtaaggcta gcatttggga ttggtacggt caagaataac cagtaggatg tagaagggtt
     4441 tagggaccgt gacgaatacc tggtatttcg gataactttt gacattgtat tggcttccca
     4501 catgtattgt gttatttgtg gattgaatgt acatttatac tcctgccttt gatgaggctt
     4561 gttaatatgt taattgtctc ctggtggtat aagcgagtat gacccacccg ttcccatccc
     4621 gaacacggaa gtgaaactac tcagcactga cgatactgcg ctggtaacgg cgtgggaaaa
     4681 taggccactg ccaggggatt ctttttattc tatccgtagg gtcttaatca aaaacccaac
     4741 cttaaaacag ccaataattt tgtgtaatta acctcaatct ggaatgcatc ctttaccttc
     4801 gcatggtgta ttacatataa ttaggttggc tgcacaagcc aaattatgac gaatggaagt
     4861 atatggatgc cacctgaaac tcaagataat tctccgcaag atatgcaaca actgctagaa
     4921 gagatggatt tcaagatttt gaggagggga gaggttgttg aaggatccgt gatgcgggtg
     4981 gactctgatg gagtctttct tgacattggg cacaaggaag aaggatttgt gccgctgggt
     5041 gaaatgaaaa ccattagcaa agaagaaatg gtgaagctca gtgaaggtga ttctttaatt
     5101 gccttcgtaa tgcgacctga ttctaaagat ggccccatac tctcggtgga ccgtgctaga
     5161 ggggaggaag gctggagaga tattcaaaaa ctaatggatc aagacgaacc tgttgagggc
     5221 gtcatagcag gatttaatcg gggcgggtgt attctggaag tcgccaatgt ccaaggcttc
     5281 gttccgatgt ctcaactaat aactatttca agagatcttt ttaaacagga atcctccgag
     5341 cctggtgggg caaatgtttc agaagataca attggaaaag atttgatcgg taaaaccctc
     5401 acagttaaag tgcttgaggt taatagatct cgaaacaggg ctattttttc agagagatca
     5461 gctttgcaag aacaaaggga tgagcagaag gctgagctta ttcagaaatt agaagagggg
     5521 gaaattcgca aaggcatcgt aactggcata agtaattttg gtgcgtttgt agatttgggc
     5581 ggtgcggatg gacttatcca catttcagag atgtcctgga gcatggttgg gtctcctgag
     5641 gatttggtaa aggtggggga agaattggat gtgtatatcc tccggataga cagggagaca
     5701 atgaagatcg ctctaagctt gcgccgactg caaccggagc cttgggaaac catatttgaa
     5761 agatatcagg ttggcgacgt tgtagatgca attgtgacca agatagccaa ttttggagcc
     5821 ttcgccaggc tggaggattc aatagaaggc ctgattcata taacagaact cagtgatgca
     5881 gtgataacga atcccaatga ggttgttagt gaagggcaag aaatcaaggt aaaaatacta
     5941 aagattgaga tggaaagaaa aagacttgga ttaagtctta aacaaacctc agaaacactt
     6001 cctcctgaag gatacatttc tgaggctcca gtcgaagatc aggaagaaac aaaaggtgac
     6061 gatggaaaag aggatagtac actttcttct gatgtgactg aacctgatgc tgctgaggca
     6121 actgaacctg atgctgctga tgtgactgaa cctgatgctg ctgatgtgac tgaacctgat
     6181 gctgctgaga ccaataattc agaagaagaa aaagatgatg tggtagaacc tacccaagat
     6241 actgaaaaag aatagctatg taggagccga tatatatggc aagtagattc gagaaatttt
     6301 cagaaagagc ccgcagggtt ttgtcattgg ctcaggaaga agctcagcgg ttcaaccata
     6361 actatattgg tactgagcat attcttcttg gactagtaag agagaacgag ggaacagcgg
     6421 ccaaagttct cggaggtctc ggtatagagt taaataaaat taggtctgcg gttgaattca
     6481 tcataggcaa gggagatact gcagctagtg gcgagatcgg cttgacgcct agggccaaga
     6541 aggtgatcga attggcagtt gatgaagctc gaagattgaa tcatcactac atcggaacgg
     6601 aacatttgct aatcgggttg atgcgagaag gtgaaggagt tgctgctggg gtcctagaga
     6661 gcctaggtgt cactttggat aaggtcaggg gtgaaaccaa ttctgttttg aataaaggtg
     6721 ctaggtcatc taaagggcgg tcgggtggaa aaacaactac aaagacgcct actcttgatc
     6781 aattgggagt ggatctgact gaaaaagcca atgatgggaa attggatccg accgtgggaa
     6841 gagagaaaga aatagaacgt gtgactcaaa tactgagtag gcgaacgaaa aacaatccag
     6901 ttttgatagg ggaaccaggc gtgggtaaaa ctgcaatcgt ggaagccttg gcccagcgga
     6961 tcgcttcaga tgatgtgccc ggaaccttgc aagggaagcg tcttgttact ctagacatgg
     7021 gttccttggt ggcagggaca aaatatagag gcgaatttga agagagactc aaaaaagtaa
     7081 tagaagaaat taagagtgca ggtaactgtg tcttgtttat tgatgagatt catacaatgg
     7141 taggggctgg ggcagcagaa ggagcggttg atgctgccaa tattctcaaa ccctctttgg
     7201 ctcgaggtga gatgcagact attggcgcga cgacattgga cgattataga aaatatgttg
     7261 agagagatcc cgccttggaa aggagatttc aacccgttag agttgaagaa ccaagcgtag
     7321 atgaaactgt tgatatactt cgaggggtca aggaaaaata tgaagagcat caccagcttg
     7381 agatatcaga ttcagccatc caatcggcag caagcttagc ctcaagattc attgcagata
     7441 gatttcttcc agataaggcg attgatctga ttgatgaggc atcatcaagg gtgcggatac
     7501 accatagcat ggcccccaga tctgtaaaag aagcttccca gatgcttgat agcgtcagaa
     7561 aagagaaaga cgacgccatt tctgatagag aatatgaaca tgcggcagaa ctgagagatc
     7621 gggaagtttc cctgtcagaa aaattggaaa atataaaaaa agaatgggaa gaagaaaaag
     7681 gggatcaaca accaatagtc gaagaggaaa atatagagga agtggttagt atgtggactg
     7741 gaattccggt tacaaggcta gctgcggcgg agagcgaaag actcattaag atggaagaag
     7801 ccttacacca aagcgtgatc gggcaacaag aagcaataga gatcatgtcg aaggctgtta
     7861 ggcgatccag atctggtttg aagaatccta gaagaccaac tggggcattt atatttttgg
     7921 gaccaacagg tgtcggaaaa acttacatag taaaaaaact ggctgagttt atgtttggta
     7981 gcgaagattc catgatacgg ctcgatatgt ctgaatttat ggaacgtcac actgttgcta
     8041 gattagtagg agcgccacca ggttatatcg ggtatgacga gggagggcag ttaacagaag
     8101 ctgtgagaag aaaatcctac agtgtgatac ttcttgacga gatagagaag gcccaccctg
     8161 aggttttcaa tattctttta caaatttttg atgatggcca tctcactgat tctaaaggaa
     8221 gaaaagtcga ttttaggaac actataatcg tgatgactag caatattggg tcagacttga
     8281 ttaggcagga caggtccctt ggattcaatg ccaggacaga gggagcccag gatgaagaag
     8341 aatcctattt gcgaatgaag aataatgtcc ttgatgaagt aaaacatttt ttcagacctg
     8401 aatttttgaa cagaattgat ggtaccgtcg tcttccatgc tttaagtcgg tcacatatgg
     8461 aagatattgt taatattttg atgaaggaag tatcaagcaa cctccttgaa aaggggatat
     8521 ctcttgaagt aacggacgcc gcccgcgaat ggctggccga ggaagggtat gatccaactt
     8581 ttggggcaag acctcttaga agattgattc aagacactgt agaagataaa ttatctgacg
     8641 cattacttgc tggggagata ggacctgcgg atacagctat cgtggatttg gatgaaaacg
     8701 acctaataat caagactgag actccagctt cactgacatc ttcctccgta tgatcacgac
     8761 ataactctga gttggaaatc gggagatctg tttgaaagca gcctacgtga atgcaatagg
     8821 tggagctagc ggggatatgt tgctggcttc tttaattgat tcggggttgg atttagtgga
     8881 attatcgtca gctctggcat cagtaggtgt agaaggattt gaactagagg ccctgtcggg
     8941 agatcgccga ggtgtgaaag ggacccactt agatgtaaat atagacaggg cggaccccgg
     9001 taaaagaact ccggaagatt ttatagatat agtcaatact gccccgatat cagaatcctc
     9061 tagatctaag tgtcgtgaag tatttaattt gattggggaa gctgaaggtt ctgtacatgg
     9121 tgaagacaaa gggaaagttc atctccatga attaggtact atagacacaa tcatagatgt
     9181 ggtcggtgtt gtattgggat tagaaatgct gtctgtggaa aagctttata gctctccgat
     9241 acctacaggt tcaggtactg ttagaaccga gcatggcctt ctaccggtac cagctccggc
     9301 aactgcctac atgttgacct caaaggatat accctgctac ccaccaccat cgtacatgcc
     9361 tcccactggt gaaatggtta cccctacagg aatggcttta ctcgcaacat tggctgattt
     9421 ttcacaaccg gtaatgaaca tacattccat cggttatggg ttagggactc gaaaccctga
     9481 atcgtatccc aatgttctat ctctttggct gggtgactta tcaactccta cagacgaaac
     9541 tcggattatc ttattggaga ctaatctaga cgataccact ggagagacac tgggatatgt
     9601 tcaagaaaaa ctttttgaat taggtgcaag agatgtatgg gttactccca tacaaatgaa
     9661 aaagaaccgt ccaggggttt tattatcaac cttgatagac gaaagcatga aagaaaagat
     9721 ctctgatttc attatgcttg aaacttccac actcggaata cgggtgagac cagttgagag
     9781 aattgaggcg gagagggaaa ttgaaaattt tgaatcgtcc cttggaaatg tgcgaattaa
     9841 attaaaaaag aaaaatggat tagtagtggc agtagcacca gagtatgaag attgtaagaa
     9901 aatagcctct gaagattcaa ttcctctcaa ccaagtgttt tttttaatga agagggaagc
     9961 agaagatatt tacctttaaa atctatgacg aattctgatc ccaaatttga cctgattgtg
    10021 ttgaccattt gtttattatg ctagtacaat ctcctgattc gatttgtatg gtgcgctgtt
    10081 ggcctaaatt ggacgctgat ttgacagccc aaccactgct cctatgttaa actgaaatgc
    10141 ggtttatttg gagggttggg agagtggtta aatccaccag actgtaaatc tggcgcccgg
    10201 aagggctacg taggttcgaa tcctacaccc tccaccataa aataactaag gtaaataaaa
    10261 gaaaaaatag gcccgcatag ctcagtggaa gagcgcgttc ttggtaagaa cgaggtcagc
    10321 agttcaaatc tgcttgtggg ctccagggat tcggaggaaa tagaattggc taagcaggtt
    10381 ttcgatagaa caaagcctca cttaaacgta ggcaccattg gtcacgttga ccatggcaaa
    10441 acaactctaa cggcagctat atcttcagta ttggcttctg aagggttggc ggatttcaga
    10501 gcattcgata cgatcgataa tgctcctgaa gaaaaggaac gtggtgtaac catcgctatc
    10561 acccacgttg aatatgagac tgacaccagg cattatgccc acgtggattg cccgggtcac
    10621 gctgactaca tcaaaaatat gataacgggc gctgctcaga tggatggtgc tattcttgtg
    10681 gttagtgctc ctgatggacc tatgcctcag actagggaac acatcctact ggctagacag
    10741 gttgaggtgc ctcgaatggt tgtggctctt aacaaagttg atatgatgga tgatgaggaa
    10801 ttgttggagt tggtggaatt agaaatgaga gaattactct cagaatatgg attcccagga
    10861 gacgatatcc ctttcattag actaagtgct ttaaacgcac ttgaagcgga agataaaggt
    10921 aaagatagtg aatgggggaa aggtgtctgg gaactaatgg atgctgttga tgaatacatc
    10981 ccagttcccg atcgacctaa ggatcaacct tttcttatgc ctgtggagga cgtgttcggt
    11041 attaagggac gaggcactgt tgtgacaggt agggttgaaa aaggtatcgt gaatgttgga
    11101 caggaaatag aagtggtagg tatcaaagac acatatacca ccattgttac cggggttgaa
    11161 atgtttcata agacccttga ctctggagaa cccggggatg cagtcggagt tttgcttaga
    11221 ggagttgata gagaggatat agaaaggggc caggtactct gcgatccggg cagtatagag
    11281 ccgcatactg aatacgaagc ccaagtgtat gtacttagta aggatgaagg cggtagacat
    11341 accccatttt tcaatgggta caagcctcaa ttttatatcc gcacgacaga tgttactggt
    11401 gaaatccaac tcccggatgg agttgaaatg attatgcctg gcgataacgt ggagatgaag
    11461 gttaagctaa ttactcctgt tgcattggac gaacaactaa ggtttgcagt gagagagggc
    11521 ggaagaacgg ttggctctgg agtaataacc aaagttcttg agtaaaatac taaatgttaa
    11581 atctagccag atcaggataa acagtctgat ctggctgttt ttcgtagaaa cgtaggaaat
    11641 atatatggcc cgtagaggag aaaaccgaat acttgtcact tttggctgta ccgactgcag
    11701 gcaaagaact tataactcca acaagagtcg aagaaatgat cctcagagga tggaattgaa
    11761 aaaatattgt cctagatgta gagctcatac gcttcatagg gaagtcagat aaattgcaac
    11821 agagatatgg cagaacctcc actagaagat caataaactt ccgatttttt ggagaagtta
    11881 ttggagaact gcgtagagtt acttggccaa caaccaacga aactttcagg ctcaccctca
    11941 tggtgttagc tgttgcagca gttttgggag tcgttcttgg agtggcggac ctggtgtttg
    12001 gttgggtttt tgacactatt ttgtagatgg tggatggtga ataccgatga ttgaggaaca
    12061 aacagaaaac aggcgctggt acataataca tgcctattct ggtcaggaag accgagtgaa
    12121 gaaaaatctt gaacaacgta tccaaaccat ggatgttaaa gataagattt ttcaggttgt
    12181 cgtccctaca gaagaggaga tggaatacaa agatggccag aaaaagacta acttgaagaa
    12241 gatatttcca gggtatattc ttgtccaaat ggagatggat gacgatagtt ggtttgtggt
    12301 tagaaatact ccaggagtaa caggatttgt atcggctgaa gacgaaagtg ataatcgccc
    12361 aaaacctgtt cccttagaac aaggagaagt cgatcatata cttaaccaaa tacgctcaga
    12421 tgaacccaga attaaagtgg gatttgcaag cggtcaaact atcagagtta cagaaggtcc
    12481 atttatagac tttatgggag ttgttgacga ggtataccct gatagattga aggtaaaggt
    12541 actagtatcg tttttcggca gggagacatc cgttgaactc gattttctac aagtggaaaa
    12601 aacttaataa ggataaattt ctcattggct aagaaagtaa taggattgat aaaactgcaa
    12661 attgaagctg gtaaggctac tccagccccc ccggtgggtc ctgcattagg acaacacggg
    12721 gttaacatta tgggatttgt gaaggagtat aacgagaaaa ctgcttctat ggcaggtgct
    12781 atagtgcctg ttgaaattac ggtttttgaa gatagatcct tcaattttat agtcaaaagc
    12841 cccccagcgt cagatttgct gagaaaagcg gcaggaatcc caaaaggtag tgggagtcct
    12901 ttggcagaga aagtggctac tattcctcgc agcaaggtta aagaaattgc cgagaccaag
    12961 ataaaagatc tcaatgcagc agatatagaa ggggctatga aaattattga gggaactgcc
    13021 cgaagtatgg gaatagttgt cgaataaccc caccctgaat aaagtactct aatttaagga
    13081 attcattatg gctaaacgtg gtaaaagata cgatacggca atagcggaga ttgatccgga
    13141 gtcaccatat gatcccaaag aggctgtttc tattgcaaaa agaaatgcta ctgccaaatt
    13201 tgatgagact atggaattgc acttaagaac gggtgcggac cccaggcatg ctgaacagat
    13261 ggttagagga atagctttgc ttccgcatgg tgtcggtaag gaaattcgca ctttggtttt
    13321 ttgccaagga gatgcgatag agagcgcaaa acaggctggg gcagattatg tcggtaatga
    13381 cgatatgata gccgagatcg aaaagggctg gcttgatttt gatgtatcca tcgccacacc
    13441 cgatatgatg ggaaagataa gtaaattggg aagaattctt ggacggagag gcttgatgcc
    13501 taatcctagg acagggacag tagtttccgc agaagacatc cctcgggcaa tagaagaggc
    13561 aaaaaagggg cgagtcgagt ttagattgga caaaagcgcc ttgattcatg caccgattgg
    13621 taaggctagt ttcgatgagg atcagttgat ggaaaaccta actaccttgg ttgacacgat
    13681 acttcgcggt cggccttctg gagtaaaagg acaattttta aggtctgcct ttttaacctc
    13741 aacaatggga cctagcgttc ctatagatat tgcggggatt atgtctctta gagtagaata
    13801 gttcagttgc catagactgc gggctctgat gttcagatta aaaattagcc tgcacaggcg
    13861 ggatttcggc aaatttgctg aaataatcaa cccggctgtg gccgggtttt tttttgccag
    13921 ttgaagtttt aaataaagat tttcggagat agatatgcct actaacagaa aggcactaga
    13981 tgtagattca cttatggaag ctatgagagg ctgtacgata gccatatcca ctgattattc
    14041 aggtttgaat gtatcagaga tgagtgaatt tagaacaact ttgagagaga acggaatcaa
    14101 gtataaggtt gttaaaaata ccctatttaa aatcgctgca gatcaagctg aaatgccggc
    14161 gattaaagat cttattgacg ggacaactgc aatcgctttt ggctatggag atgaacaaat
    14221 tcctgccaaa gtaattacta aatatgttcg agattcaggg gtagagttaa aaataagaaa
    14281 tgctttgatt ggagacacaa ctctatcctc agaacagctg aaggagctgg ccgctttacc
    14341 tggtaaagaa gaattggccg cgaagttggt cgggcagttg tatggacaat tggctggatt
    14401 agttcatgtg atgaataatc cattacttag gttggctaag tccttgaatt ccccgtctag
    14461 tggcttggta aacgtgctga atagccgtgt tcgacaaatt tcttaagaag aaacacaaat
    14521 taataaatag ttaggagagt attagttatg gctttatcta aagatgaaat tctagaggct
    14581 ataaaggaat tacctgttat agaactagct ggattggtca aagatctaga agaggaattt
    14641 ggggttagtg ccgccgctgc tgcacccgtg gcagttgctg ccgcaccggc agccgctgga
    14701 gactcagcag gggctgctga ggaagaacag gatgccttca ccattaatct caaagagatt
    14761 ggcgagaata aaattaacgt tatcaaggca gttagagctg ctacctcgct aggattaaag
    14821 gaagcaaaag acctcgtaga aagtgctcca gttgcggtca aagaaggggt tggcaaggaa
    14881 gaggcagata acgtaaagaa agaactagaa gaagctggtg ccgtcgtaga attgatctaa
    14941 ggcatattgg atctcctaga tggttttaac agttagtggg gcggaccttt ctacattgtc
    15001 cgtccctatc taccccactt agaaagggga tagtctgagg tccttcaggt aacacacaga
    15061 ctgttccttt ctggtacttt ttagaaagta gttcacgcac cttcattgaa acatcttcag
    15121 ttggaagaag gtgagcctca acaatctgtt ctatggacag gttatctgac ttgatataga
    15181 catcattttc catttgtata gaggcctgta tttggacctg ccattgatca tgtttctggt
    15241 aatctgagga ctcaatcata ttaataagtg attgagggga tgtttgagat ttgagtattt
    15301 ccccataaga accgtgattt ggtatgccat ctgagcattc agcggcacag ataatactcc
    15361 cacctttttt tacaatttga tgggcagcag acatcccttt cacagcctga tataagttca
    15421 tatctaaggg atatccactg ttggttgtga caactatgtc aaacggttcg gagacttccc
    15481 tcatcgcggt ggaatgagcg aattcacatg ctgccttatg aacctgaaac atctctccac
    15541 catagacact cgttatttta tggtttttat tgatagtgac atctaatgtg aaatcggatg
    15601 gcgacaaagt ggcaatttct cttatggaat cgtgtatagg gtttccgtag gttatacccc
    15661 attttgattt agtgtttcca atcatgtctg aatcatggag tttcatgatg gtttcaaacc
    15721 cggccaaccc cggagcaatc atttttgggc ctccactgaa tccagcaaaa aaatgaggtt
    15781 ccacaaatcc cgtggtaatc ttaaaatctg tttttatcca gtcagagttt aggaataccg
    15841 gtatattttt tttagtgact cctacctccg ttaaattatc tcgattgaag gcatcgtgat
    15901 ttactatgtt atagccaccg ctcataggtt gttcacccaa catggaaatt aattctgaat
    15961 gtgtgttttt gcggtgagtt cctgtcgcaa taaatatggt tatgtttgat ggaaggatat
    16021 gttttaactc atttaaaatg ataggcaaaa cccttttatt aggcataggc ctggttatgt
    16081 cacaaacact tattcctata ttttgtcctg gcgctacgga ttttctaaga ggggctgttc
    16141 caattggatt tcgtagcgct gttttaatag cccctagttc gtctggcaaa ccgggaataa
    16201 attgaggttc gattaccgaa gttcgatcat caggtagatc aatagttagg cctgttttgc
    16261 cgtacgctaa atctattttc atacataggg cctaaacata ttatttagtt cttaaaaggg
    16321 cttcacaaag atgtgaggca tctaccaaat cagggattgt tacgtactct cgtactgtat
    16381 gcatgccatg gtctgccata cctaccacaa ctgaactaat tccattcagc ctaaatacgt
    16441 taccgtcagt tccgcctcct gatggcttca tacttgggtc taatccaagg cctttcaggg
    16501 tttctttaac tctcatggtg gctggatcat cgtcagtcaa ggtataggtt tcaaattcta
    16561 catgtagatg attatctatt gtcgcctcgg ggaatagttt ttctacttct tttacaacat
    16621 ctgttatctg aacccttaat ccatctaaat tttctatact tgtactgcga aactccccgc
    16681 gaattgtagt tgtctcggga acagcattcc taactgtgcc accttcaata gttcctacat
    16741 tcaccgttgt gttttcatcc aatcttcctt gcgggagtcg tgtgataatt tctgaggcta
    16801 ttttgataga ggaaaggcct ttttcaggtt ccactcctgc atgggcagct ctgccagtga
    16861 tttctatatc aaatcccaca taggttggac tcgatgccgt tatttggctt accggtcctt
    16921 ctccatcaaa tactatcgcc tctttggcgg ttattagatt gaagtctagg ttgcgtgcac
    16981 ccactagtcc tatttcttct tccctggtaa aggcaagttc tactggatga tattcaaccc
    17041 catcttccat gatcgattct aaggcttcaa gaatggccga tactccagcc ttgcaatctc
    17101 ctcccaaaat agtgcttccg tctgacacaa ttctatcgcc tttcactgat ggttttattc
    17161 cccttcctgg ctctactgtg tctaaatggg ccgataaaag gatggggtct ggccgttcgt
    17221 ctgatgcaat taggtttcca tatgcgtccc tcattgtttt aaaccccaat gtttccaatc
    17281 gggaggaaat attaagagcc atatcttctt cttcacctga agggctatca attcttgcta
    17341 aatcgcaaaa tgtttgtaca agtcgttctc ttctaatcat gtttaagctc ttattgtatt
    17401 ttatttattt ataacggcag taatactaat atatgcattt aatacggtcg atatttgttg
    17461 gaaataggca tcctgcgatc cctaccaaaa ttcctagaag ttattttgat acctggagct
    17521 gactgacgcc gtttatactc atttatgtct acaagtttta atatttcagc cacggtgccg
    17581 ggatcaaatc cgatatttaa gatatccgca attccaagat cttcttcgac atatttttcc
    17641 aagatagcat ccaagatatc atagtcagga agagtgtctg aatctttttg gttaggtttt
    17701 aattccgcgc tagggggttt ggtcaagata gaattgggaa ttaagtcgta tccatgcttc
    17761 atattaatat agtggcaaat ttcgtacacc atacctttgg gtaagtcctt tattactgag
    17821 aatcctcctg ccatgtcccc ataaatggtg gcatatccga cagccatttc acttttattc
    17881 ccggtggtta ataccagaaa tccgaatttg ttagataatg ccatcattat attgcctctg
    17941 attctagatt ggaggttctc ctcggcgacc ccccattcgg ttccttgaaa ttgatcattg
    18001 agaccttttt ccatagattg aagaatttcc tcaatatgaa tcgtgtccat tggtatttct
    18061 aaattatcag ctagtttttt ggcgtcaact ttgctaccac tggaggaaaa tttggaaggc
    18121 aaagatatac cttttacatt ttccgagcct aaagcctcta ctgcaatggc ggcaaccagg
    18181 ctagaatcaa tacctccgga tagagcaatt agggccttag tgaatccgca ttttcttacg
    18241 taatcgcgtg ttccgagtac cagagccctg taaatttctt cgttctgatc aataattgtc
    18301 agtggagtta agggaatatt agaggagctc tcttttatta tccccggtaa gatcaccttt
    18361 tttatctcgt taactgagtc ctgcgtagta ctaggcttat atgtaggaag atcaatttca
    18421 tatatatata tattttcttc gaattgattt gctctcatga gtatgttgcc tgctggatca
    18481 actatgagac taccgccgtc aaacacaagt tcatcttgcc ctccaaccat gtttatatat
    18541 gcgatgaata caccattgct ttttgctctt tcgatcaata ctttttctct ttgagatctt
    18601 tttcctaagt tgaaaggaga accgttgata ttgataatca aagatgcccc attatcactc
    18661 tggatatcac ttggaccggg tgaaaaccaa atatcttcac atacatttac tccgaacctc
    18721 acatggtcca agtcgtatac cgggaaatca gacccttcag tgaaataacg tttttcatca
    18781 aagactccgt aattcggcag atacatcttt tggtagctat ccaggatttc gtggttgcta
    18841 actatcgcag cagaattata gattttgtct tccacacgtt ccgcatatcc gaatatagcc
    18901 gttatttcac cggtagatcg agctattctt tcggcagcat ccatgttgtc ttttatgaaa
    18961 ttatgtttaa gcaagagatc ttctggagga tatcctgtca ccgccagttc tgggaaagcg
    19021 ataatgtcaa ttccttcctg ttctgctttc tctatatggt gaatgatgac gtcactgtta
    19081 tgacttaaat caccgactgt agggttaatc tgcgctaatg ctaaacgtag ggtagccaat
    19141 tctagtatgg tccatcaaca taaattaaat aaaatatacg ggacctattc tactgtaatc
    19201 ggtgctgatt taggttttgg attctacgaa gttacgtatt ctgtctatgg cttcgatcaa
    19261 attttctgtt gagtttgcaa aagatattct tatataaccg gttccgtatt caccaaacga
    19321 ttcccccgcc aataccgcta ctccggcttc ttcaaggact ctattagcaa attcagagct
    19381 agtcattccg gttccttcta cgttggggaa cgcataaaag gcacctttag gtaaagcaca
    19441 tgttataccg tcaattttgt ttaacccatc aaccactata gatcttctct tcttgaattc
    19501 ttcaaccatt agtattgaat cttcttgagg cccttctgtt gcttcaatag cggctctttg
    19561 ggtgaaactg gctgtgcatg acacactgtt ggtcactaac cgtgaaatag gttcaaccaa
    19621 gtcatctgga aagaccccat aaccgattct ccaaccagtc atagcgtaag ttttagagta
    19681 gccgtcgagt attatggttc tatctatcat gtcagggaaa cttgcgatac tgtggtgtgt
    19741 tccttcgtat aggaagcgtg agtatatttc atcagataaa actgtaatgt cattttcctt
    19801 agctagtttc gacaaagctt ctaaatcttt tttgtctatt acgcttccac atgggttatt
    19861 aggagaattg acaatcagca attttgtttt ttcagatatc tggctttcca actccttaat
    19921 atctaaattg aaagagtttt cctcaccaag tctcactggg actgccgtcc cgccgcaaaa
    19981 ttcaatcata gattcataaa ttgggaaccc cgggttcggg taaagcactt catccccatt
    20041 ctctatcatc gccagtatta tgaaaaacat tattggttta ccacctgggg tgacaatcac
    20101 gttttctttt ttgacacgta tattcctact tttagatata tcttttgcta ttgactcttt
    20161 caaatcggca tatccgggag aagggttata tgaggtaaac ccatcggaca aagcctgcct
    20221 gcctgcctct acaatgttag tcggggtatc aaaatcaggt tccccgattt cgagatggat
    20281 gatatttctt cctttggctt ctaactgttg ggctttcgct agtacttcga aggctgtttc
    20341 agttccaagg cgactcatcc gctgagctaa tttcatatgg ggtggaatct ccgttgcgac
    20401 agttagatat gattagtata caacgactca aaggttacta ctacaacggg ctcatcaacc
    20461 taaccatatg acattatttg atatgctaga ccaagtattg ggtatatatc atgctaggag
    20521 ggctaagtga acgtttattt gggagtcccg cgagggtttt gtgccggagt ggttcgcgca
    20581 atagatattg ttgaattggc ccttaaaaaa tatgggcctc cgatatatgt gaagcatcaa
    20641 atagttcata atcctcacgt tgtgaaggat ttagaatcga aaggtgctat aacagtcgaa
    20701 aatgtaaatg aaataccaag aggatcaaag gtcgtttttt cggctcatgg ctctcctcct
    20761 gaagatttca agaatgcaaa agaacaggga ttacaagttt tggatgcaac ttgccctctt
    20821 gtgattaagg ttcataacga agcaaaaaaa tacaaccggg acggcagaaa aatcatattg
    20881 gttgggcata aaggccatca ggaagttaaa gggacaacgg gccaaacaga tatgaaaatt
    20941 gtggatgata gggaggaatt tgtacctcca aaatgggata attccattcc cgtggctgtg
    21001 ttgacgcaga ccacgctttc tgtagacgat actgaaaatt cgatacgcaa tattcaaaac
    21061 tcctttactg atgtgttagt aaggaatgat ctctgttatg ccactacaaa caggcaaatg
    21121 gcagtaaagg aaatttctaa tcatgttgag gtatttttgg tggtgggtgg gcaaaatagt
    21181 tctaactgca atcgattaaa agaggttgcg gaatcaaacg gagtccctgc ctttttgatt
    21241 aataatgtag aagaacttga tcctaaatgg cttgctggcg tggtaaacgt aggtataacg
    21301 tccggagcat ctacacctga ctcactagtg aggggcatca tagatttcat tgaccctgac
    21361 aatgtaattg aaatgggggg cgaagaagaa aatataactt tcaccttgcc taaagaatta
    21421 agagatatag gggacgcata aattggaaat aagaatcgaa agtctgattg cgggagccag
    21481 agaggctgag ggagtcgtgg tggtggtcga tgttttccgt tgttttacta cagaagccgt
    21541 ggcttttgct aacggcgcaa aggaaatcat cttggttgct gaaatagagg aggcttttga
    21601 acttaagcgt aaagggatgg gggatgtgtt gatgggggaa gttggaggta aaaggccgga
    21661 aggatttgat tttggaaatt ccccttttga attaaaagat gtagacctga ccgacaagac
    21721 aattatccag tccacaaggg caggaacagt gggtgttaca gcggcttcta aggcagatgt
    21781 catctatgga ggttcgtttg ctgtggcatc tgctacggca actaacattc tttcggcaca
    21841 gccagagata gtaactatag tagccatggg tttagaaggt aagataagag cagatgaaga
    21901 tgaacaatgt gctctttatt tacggaatac catgcaaggg agaacccctg atcataaggc
    21961 tgtaagatca ttggttatat ctggtgaaga gtcgcaaaag tacgaaaccg ctgaataccc
    22021 tcaatggcct attgaagatc gtgaaatggc tttggatata aattcacact catttgctct
    22081 tatgattaat aaagaagacg gatattttgt atccaggcct gaaaatatag taaataacgg
    22141 gagactctaa tgaaatatag aaaattagga aaatctggct tggaggtttc tgttgtcggc
    22201 cttggggcta ataattttgg aggtaggttg gattacgaga gttcgaactc cgtgatcagt
    22261 caattatttg atgttggaat caatttgatt gacacctcaa atagctatgg taataccatg
    22321 tcagaggaat atattggtag gtccctagaa ggtaggcgtg agaaggccgt aatagctaca
    22381 aaggtttcaa gtcgtatgtc tgaaggcccc aatcaagctg gcaactccag aaaacatata
    22441 atagaacaag ttgagaacag cctggctcgc ttgcgtaccg attatataga tttgtaccaa
    22501 attcattggt gggaccccaa taccccgata gaagaaacct tgagagtttt ggatgatttg
    22561 gtcacccaag gcaaagtaag gtattttggg tgttcaaatt tttctgcatg gcaggtttgc
    22621 gagtctatat ggacctccag ggaacttgga atccagactt tcataagtgc ccaaccacac
    22681 tacagcatgt tagaacgaaa tgtagagcag gaactgattc ctttttgtgg aaagtatggg
    22741 gtcggaattc tcccatattt tccattagcc aatggatttc tgacaggaaa atacaggaaa
    22801 aattctccac ctccagaagg gtctagattg gaagctgaca ataaagggct gtttacggag
    22861 gataattatg cgctactgga aaaattgatc aatttttccg aagagagagg taaaactgtt
    22921 ttggatttgg cgttcgcctg gctactttat aacaatgata ttagttcagt aatagctggt
    22981 gcaaccaaac ctgaacaggt tatctccaac gctgacgcag cagattggca gttgactgat
    23041 aaggaatatg aagaggtcac ccaatttctt ccttaagtta tattagggtc ggtttgccag
    23101 aatctggggt tacctcaaaa acattgaggg cacatgctag catcccgtaa tagccaatgg
    23161 ctgccgttaa ttctgtgacc ccctgttctc cgtatttgga aacagcctga tcaaacgttt
    23221 cttggtccac ttttttatca tttattaatt gtcttgcata acgtattacc agtgaatagt
    23281 tgggggcgac cttgtttgtg gaagttcttt tatttatgac gttcacaact tcttgggtta
    23341 caccagcctc catagctatt ggcacatgag ctgcccattc gtaattacaa ttcatttcac
    23401 gggcagctgt aatgatcgca acctccctct catcgtcctt aagtaaagat tcaaagcgta
    23461 tgtaggcccc aagatgcgcc gctcttccgg cgacctctgg gctgtgaagc agaactgaga
    23521 atgggccact tatccttcct ctggaagaag ctatggaatc gaatatttca tgatgttgat
    23581 ctgacacttc attttttgat gttatttgct tgattctagc catactattt acctctaaat
    23641 tgctctttaa aatttatctt ttttaaaatc acacatccgg caattatcac caccgagata
    23701 attgatgtta tgagtgcagt acccgatatc cctgctataa actttgaaat cgcaattttc
    23761 ccaatgctgg tgacttgctg aagcactaaa gattcagtgg ctaagaactg aggtgcgatt
    23821 tgtccgctag tttgtgatgc gatttgatct atttgtacgt gtatgattgg ctctgcgaaa
    23881 gaagagtaaa tcggtcccca gattacgtat acaagaaatg atgcgatcaa catagctata
    23941 gcagcccaca tgagtctttg attccatcct cgtccgccta aaaaccccac tagagctgct
    24001 attagaatag tcggtatgta gactgccaaa ttaaacatgt gtactagctt aagtatgcct
    24061 cgaagttggt caagcatcga tagtgtttgg ccaccatctg cggttattgt ttcaatatga
    24121 accctaaaat cttgatcgtt atattgaatc ccctgtgata acgaatttct aacagtgttg
    24181 acttgatccc aagaattgtc accagcacct tgtttgataa ggctttccaa atcagtttcc
    24241 ctgaaggtat atccttctcg catgatggct ctaccctggt caaaagaatc taatgctttt
    24301 ggtgggacta cctgcctcaa ggagtcttga tcaaattcga ttaagtttgg aattgtattg
    24361 ataatctggg gtcttactgc tgagatgact gtacttatgt atgctttggt ataactctgc
    24421 atttgtcctt taagggctgg atctgcgggg tagcatgatg gaattcctcc gacggggttg
    24481 ctcaatatat tggcgacttc agccgcgtca caatctggta aattgtccaa tttgctgttt
    24541 aacttttgct cggcgagcgc catcaaatca ctgaccgcat cctctttatt tggctctatg
    24601 tcaattgata tagtaaattc atcagttctt ccgaccaaat acggggtggc attatcaagt
    24661 attgattcag tggttttttg cacccaagca gctggggctg tcttttttat tattgctgat
    24721 atttcctcgt cagtaatttc cacaccatag ggcaattttg cagtgttgcc tattgaagag
    24781 gatatatttg gagcaattga accttcaaat aatgattcat atgcgttggc ttcagccaat
    24841 aaaaatttga cttctgcgac tgctacttca attcgatcgt ctacagttat cacgacagcg
    24901 aaagtatctt tagctcctac cgcgtaagga actacttcgc cgaggaccga atctatttgc
    24961 cctgtgagcc atgatttaga tattattttt tggatcgaac ccattagctg gtcctcgcta
    25021 acagatacgt cgaaaggtaa ttcgtttttc gacgcttgag acacaactgg gcgaacttga
    25081 ttttccatga caagtttata aaggtcactt tcatttaaaa taaaagttat ctggttagag
    25141 gctgcttgca ctctttcatc taccggtatc gaaatggtga aatcgtcact ttttccagtt
    25201 acatagtcac cgacccctgt gacggtggat tcaaaattgg attgcaacca ctcaggtggg
    25261 ataatttcgt ttatcgaggt gattataatt tcatcagata atccgctctg atcaataata
    25321 gggttactat ttgccttccg cacgtctttt agagctgttc tgggcaaatc agtcaataga
    25381 aactggtaga tgttagactc tgccaaagtt tccaggtaga aagatggatt gatgagattg
    25441 gaagctgtct gctgggcaag aacaccaact tgaaaggtga cgaatgccat gattatgaaa
    25501 ggtaatgcaa ttattctacg tacccaaatc atggcaactc cattgctaac aaagtttgaa
    25561 tcaagaaact aaatcaatat aaatagaacg ctgttattta gtgtttgtgg ctcacggcca
    25621 cggtcatcta ataacttcaa ccgactccat ggaatcctat ttctacatta tctcctgtga
    25681 ccataaccgg agttattctc tctccccccg ttaattcttc tactttttcc cagagttcag
    25741 ggttaagggc tagatttatt tcttcgaaat ctagtgctaa atctataagt tcttctttta
    25801 aggcgtctga atacgtacaa tccgggctag tgtataaaac tatggtatct gacataaaac
    25861 acctctgatg ttagccgcaa tttgttttgt cggccgaatt atattactaa atgagtatct
    25921 atagtttgtt attcatgggt tgccgaattt tattggccat aatttcataa caaattcctc
    25981 taaaatatac tcacatttga tagctcctaa gatgcggctg tctaaatttc ctgatagacg
    26041 attatcggac aagacaataa cggaatcatt ct
//
LOCUS       DASCNS010000153         6039 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001215316, whole genome shotgun sequence.
ACCESSION   DASCNS010000153 DASCNS010000000
VERSION     DASCNS010000153.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001215316"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(35..1651)
                     /locus_tag="QF838_05635"
     CDS             complement(35..1651)
                     /locus_tag="QF838_05635"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60196.1"
                     /translation="MAGLNGAQAMFQQLVMEGVTDIFSLPGAQIMSAFDVLHEMQDQI
                     NLVHTRHEQATTYMADGYAKVSGKPGVAMVVPGPGALNASAGLGTAYASSSPVLLISG
                     QIPSTLLGKDTGQLHEVSEQLDVFKPITKWNHRISDVSEVPAAVHEAFRQMTTGRPGP
                     VELEIAPDILTQPGSVDLVEKELYPHPKATREQIIAAVKLISSAESPTIVAGGGSVSS
                     GASEQVRRLAELLQIPVMTSPQAKGIIPEDSHLAAGVNSAVGPAKTVLPDTDLVIAIG
                     TRLSLRGISADEMPPVLQIDVEQDQIGKQYPAELGIVGDAAETLTALIGALSVDDSKL
                     PKRRERAERLKKQFADEVIRLASPQVSVIDKISNVLPDNAIVVQGMTNIGYWSSAALP
                     MDNTRNYVTSSYFGTLGYAFPTALGCQTAFPERKVVALCGDGGFMYSPQELSTAMRYG
                     LNTVAVVFNNGAYGASKWDQQHQFNGRFIGTDLFNPDFVAMAKSFGAEGIKTDADSIE
                     EGLAEALALDKPTLLEVEIQNMMPPFQIVE"
     gene            1765..3132
                     /locus_tag="QF838_05640"
     CDS             1765..3132
                     /locus_tag="QF838_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HJO60197.1"
                     /translation="MQSSNSDSVKFLLEASIVGMLVKLAIPNSIAIITMTSIILSDAK
                     FVGQLGTTALASLAVVFPFQSLLQMMSAGAIGGGVASSLSRALGAGDKEKAEEAAWHS
                     LLIMVVMAAMYTLVLGIFCRPIFSILGARGEVLDGSVSYARVLFGAAFMGWLFFVGSS
                     WLRAIGQIFLLSKIVIISSISQIILSGSLTLGWGPFPAIGISGPAVATVMCHSVAGGY
                     MVYVMTGNKLDIKLRPYKFNIRAVRDIMKVGGIGLINSTSIALSVVVVTWVIGRYGDE
                     ALAGYGLGSRLEIILTPIAFGIGSIVTIAVGANFGAKQFMRARKIAWMGCAATFVITS
                     VIGLGSAIEPGLWLDRFTSDPEVYKHGALYFVIAGPFYGFFGGGQALYFANQGTGHMV
                     IPVLISLARLILVCSVGILSVLLLWDLSVIFWGVGIGLLVIGTGLSLNMFGPVWQPKA
                     PSVGK"
     gene            complement(3139..4107)
                     /locus_tag="QF838_05645"
     CDS             complement(3139..4107)
                     /locus_tag="QF838_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012568.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HJO60198.1"
                     /translation="MQAFSILNPISEQLQLVDARLRTFSESSELPSLKDLLIHVFDSS
                     GKLARPALTLLTAGFHPNDGEKVITMATAVEMLHVATLIHDDTVDESNTRRGRVTVSS
                     SWGQHTAVLLGDYVFAASATYVCSTGNIRVIKRFSETIMDLSRGQLQETANSRDITKG
                     VDDYLERIYFKTASLFSTSGESGAVLSGAPEPIVDSLKKYSEKIGLAFQVVDDVLDIT
                     GTPQEIGKPVGSDLLNGVITLPTIYAIEEPDCKKKVGLFLDDPSNIELHSEIVEIIKS
                     SDAEARSYKYADQLVEEAKLSLQPLRDNDFKKSLLDLADFIISRNN"
     gene            complement(4120..5790)
                     /gene="metG"
                     /locus_tag="QF838_05650"
     CDS             complement(4120..5790)
                     /gene="metG"
                     /locus_tag="QF838_05650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HJO60199.1"
                     /translation="MPENILVCVAWPYANGSIHLGHVAGAYLPADIFARYHRIRGNNV
                     IMVSGSDAHGTPVTITAENEGISPEQVAKKYQTEFLNDWDGLGINWDLFTTTHTQNHF
                     EVTQDIFSRLHEKGFIYKDTMSQPFCEEHNRFLADRYVEGICPNCNSNGARGDQCDGC
                     GNTLDPKDLIDIKHKDCESTPVFRDTEHFFLKLSAFEEDLIEWMRENSHWKPNVKNFT
                     LGFLEGGLIDRAITRDITWGIPIPLEGYEDKRIYVWFEAVIGYLSASIEWASKTDNPD
                     QWKDYWKEDSKPYYFMGKDNIPFHTVIWPAMLMGYGGLNLPYDVPANEYVNMEERKIS
                     TSRNWVVNLKDYLERYDPDPLRFSLASIMPETSDSNFSWSEYVRRNNDELVATFGNLV
                     HRVLSMTTRYFDGVVPGPKNKDNLDNLLINEANSTLSLVATELENCRFRKALEYSMSL
                     AQETNKYLDDKAPWSASKTDLESAGTSLYYCLNVINCLKTTFSPFLPFSVEKLHSMLG
                     FEGSANDNGWNWDPDEVIPGQKLGEPKALFIKLEESVIDEEISRLGLN"
BASE COUNT         1562 a         1311 c         1307 g         1859 t
ORIGIN      
        1 tcttatgacc cggattgaac ttgatataga ttaactattc tactatttgg aagggcggca
       61 tcatgttttg gatctcgacc tctagtaggg taggtttgtc taatgcgaga gcctcagcga
      121 gaccttcttc aatactatca gcgtcggttt ttataccttc agcaccaaag gatttcgcca
      181 ttgcgacaaa atctgggttg aacaggtccg ttcctataaa tcggccattg aactggtgct
      241 gctgatccca ctttgaagca ccgtacgccc cattattaaa caccactgcg acagtgttga
      301 gcccgtacct catcgcagta gaaagttcct gagggctgta cataaaacca ccatcaccgc
      361 agagggccac aaccttcctt tcagggaagg ccgtttggca acctaatgct gtagggaagg
      421 cgtagcctaa agtcccaaaa tatgatgatg tgacataatt tctagtgtta tccattggta
      481 gagccgcgct actccaatat ccgatgttgg tcataccttg tactactatt gcattgtccg
      541 gtaagacatt ggatatctta tctataactg aaacttgcgg cgacgcaagc ctaatcactt
      601 catctgcaaa ttgttttttc agtctttcag ccctttcacg gcgtttgggc aacttagagt
      661 catctacaga taaagcaccg atcaaggctg ttaaggtttc cgcagcatct ccaacgatgc
      721 ctagctctgc agggtattgt ttacctattt gatcttgctc aacatcgatt tggagaactg
      781 gtggcatctc gtctgctgag atgcctctga gagatagtct tgtacctatg gctatgacta
      841 aatctgtgtc tgggagaact gtcttggctg gaccaacggc ggaattgacg cccgctgcta
      901 aatgactatc ttctgggata atcccttttg cttgcgggga ggtcattacg ggaatttgaa
      961 ggagctctgc tagccgacgt acctgttcag atgcaccaga ggaaacactc ccacctccag
     1021 caacaatagt aggagattcc gcagatgaga tcagttttac tgcagctatg atttgttccc
     1081 tagtggcttt agggtgtggg tataattcct tttcaactaa atcgacactc cctggctgtg
     1141 tcaaaatgtc gggagctatt tccagttcaa ccgggccggg ccttcccgtg gtcatttgcc
     1201 tgaaagcctc atgaaccgct gcaggaactt cagaaacatc agatattcta tggttccatt
     1261 tcgttatggg cttaaagaca tccaactgtt cagatacttc atgaagttgt ccggtatcct
     1321 ttcctaaaag ggtactgggg atttgccctg aaataagaag caccggactg gaagaggcat
     1381 aagcggtccc aaggccagcg ctggcgttca gggctccagg cccaggcact accatcgcaa
     1441 ctcctggctt acctgagact tttgcgtaac catcagccat atatgtagtt gcttgttcgt
     1501 gccttgtgtg tacaagattg atttggtctt gcatttcatg gagaacatca aaagcagaca
     1561 taatttgagc accaggcaag gaaaaaatgt cagtgacacc ttccataact aactgctgga
     1621 acattgcttg agcaccgttt aaaccggcca tttttcacct tctcgaacac ataagttcat
     1681 aagaatttgt tggattatct cacatgggtt ttcaatatca tacaattgtt aggttgccaa
     1741 attttaggaa aagagttttc ttcaatgcaa tcatcaaatt ctgacagtgt taaatttcta
     1801 ttagaggctt ctatagttgg gatgttagta aaattagcta tcccaaattc cattgcgatc
     1861 atcaccatga catccattat tttgtcggat gcaaaatttg tcggccaatt gggaacaacc
     1921 gcattggcta gcctggcagt cgttttccct ttccagtcgc ttcttcaaat gatgtccgcc
     1981 ggtgcgattg gaggaggggt agcttcttcg ctttcgaggg ctttgggtgc cggtgataag
     2041 gaaaaggccg aagaggcagc atggcattca ctattgataa tggtggtgat ggctgcgatg
     2101 tacaccttgg tgttgggaat cttttgccgg cctatattct caattctagg ggctagggga
     2161 gaggtgctgg atggctctgt ctcgtacgca cgagtgcttt ttggagcagc attcatgggt
     2221 tggttgtttt ttgttggttc ctcatggctt cgggccattg ggcaaatttt cttactttct
     2281 aaaatagtaa taattagcag tatttctcaa atcatactat ctgggtcact tactttaggc
     2341 tgggggccat ttccagcgat tggtatatcg gggccagctg ttgctacagt gatgtgtcac
     2401 tcagtggctg gaggatacat ggtttatgtt atgactggga acaaattgga tataaaatta
     2461 agaccctata aattcaatat acgtgccgtt cgagacatta tgaaggttgg tgggatcggt
     2521 ctaatcaata gtacttctat agctctctca gtggtagtgg taacgtgggt catcggacga
     2581 tatggagatg aggctcttgc ggggtatggt ttaggaagcc gattagaaat tattcttaca
     2641 cccatcgcct ttggtattgg tagcatagtg accattgcgg ttggggctaa ttttggtgca
     2701 aaacaattca tgagagcaag aaaaatcgct tggatgggtt gtgctgctac ttttgtcatt
     2761 acctcagtca ttggattagg atcagccatc gaacctggtt tatggcttga tagatttacc
     2821 tcagatccag aagtctataa gcacggagca ttgtactttg ttattgcagg gcccttttat
     2881 gggttttttg gaggaggaca ggcgttatat tttgccaatc aaggtactgg acatatggtt
     2941 ataccggtcc tgataagctt agcgagatta attcttgttt gttcggttgg tattttatcc
     3001 gttctattat tatgggatct ttcagtaata ttttgggggg taggaatcgg actactagtt
     3061 attggcacgg gactttcgct aaatatgttt ggtcccgtct ggcaacccaa agcaccatcg
     3121 gtcggtaaat agacatgctt aattgtttct agaaataatg aaatcagcca aatctaatag
     3181 agacttcttg aaatcattat ctcggagtgg ttgcaaagat agtttggctt cttccaccaa
     3241 ttggtcagca tatttatatg atctcgcttc ggcatcggag ctttttatga tctcaacaat
     3301 ctccgaatgt agttcaatgt ttgatggatc gtcaagaaat aaccccactt tctttttgca
     3361 atctggttct tcaattgcat atattgttgg caatgtgatt accccattca ataaatcact
     3421 accaaccggc ttacctattt cttgtggtgt gcccgtgata tccaagacat cgtccactac
     3481 ttggaaggcc agtcctattt tctcactgta ttttttcagg ctatccacaa ttggctcagg
     3541 agctccgctt aaaacagccc ccgattctcc tgaagtcgaa aatagtgaag cagtcttgaa
     3601 atagatacgt tcaagatagt catccactcc cttcgtaata tctctactat ttgcagtttc
     3661 ctgcagctga cctctagaaa gatccataat tgtttcagaa aatctcttta ttaccctaat
     3721 atttccagta gagcaaacat atgttgctga agccgcgaag acgtaatccc caaggaggac
     3781 ggccgtatgc tgaccccaac tggaactcac tgtgactctt cctcttcttg tattcgattc
     3841 atctacagta tcgtcgtgga tcaaggttgc aacgtgcagc atctctacag ctgtagccat
     3901 agttattact ttctccccat cgttgggatg aaacccggct gtgagtagag ttaaggcagg
     3961 ccgggctagt ttgcctgagg aatcgaacac atggattaat agatctttga gggagggtaa
     4021 ttccgaactc tcagaaaatg ttctcaatct agcatcgacc agttgaagtt gttctgatat
     4081 tgggtttagt atcgagaatg cttgcaatag aagaagttcc taattaagac ctaacctact
     4141 aatttcctca tctattacac tctcttctag tttgatgaat aatgcctttg gttcacctaa
     4201 tttttgccca ggaatcactt catctggatc ccaattccat ccattatcat tggcactgcc
     4261 ctcaaatccc aacatgctgt gaagtttttc cacactaaag ggaagaaacg gtgaaaaagt
     4321 ggtcttaagg caatttatta catttaaaca atagtagaga ctagtgcctg ctgattccaa
     4381 atcagtttta gaagcactcc aaggtgcctt gtcatccagg tatttattgg tttcttgggc
     4441 taaagacata gaatattcta aagcctttct aaatctgcag ttttctagtt ctgtggctac
     4501 caatgacaaa gtgctatttg cctcatttat caataaatta tcgagattgt ctttattttt
     4561 gggtccagga accacaccat caaaatatct ggttgtcatc gacaaaaccc tgtgcactaa
     4621 gtttccgaaa gtggcgacaa gttcgtcatt gtttcttctc acatattctg accatgaaaa
     4681 gttggaatcg ctagtttcgg gcattattga ggctagggaa aaccggagag gatctgggtc
     4741 atatctttcc aaatagtcct ttaaattgac aacccaattt ctacttgtag atatctttct
     4801 ttcctccatg tttacgtatt cgttggcagg aacatcgtat ggaagattca aaccaccgta
     4861 ccccattagc attgctggcc aaataacagt gtgaaacggg atgttgtctt tgcccataaa
     4921 ataatacggc ttggaatcct ccttccaata atctttccac tgatccggat tgtccgtttt
     4981 tgaggcccat tcaatagaag cagataaata gccaatgaca gcttcaaacc atacatatat
     5041 tctcttatct tcgtaaccct caagagggat aggtattccc catgtaatat ctctggttat
     5101 agctcggtct atcaatcctc cttccagaaa tcccaaagtg aaatttttaa cattaggttt
     5161 ccaatgggag ttttccctca tccactcgat caaatcttct tcgaaagcac tgagtttgag
     5221 gaaaaaatgt tcagtgtccc taaagactgg tgttgattca caatccttat gtttgatgtc
     5281 tatcaagtcc ttagggtcaa gagtattacc gcagccgtcg cactggtcgc cccttgctcc
     5341 gttagaatta cagttagggc aaataccttc cacataacga tcggccaaaa acctgttatg
     5401 ttcttcgcaa aagggttgag acatagtgtc tttataaata aacccttttt catggagtct
     5461 tgagaatata tcttgcgtga cttcaaaatg gttttgggtg tgtgtcgtgg taaaaagatc
     5521 ccaatttata ccaagcccat cccagtcatt gagaaattcg gtttgatatt tttttgccac
     5581 ttgctccggg gatatacctt cattttcagc agtgatggtc actggggttc catgggcatc
     5641 gctacctgac accataatta cgttattacc tctgatccga tgatatctcg caaaaatatc
     5701 tgccgggaga tacgctcctg cgacgtgtcc aagatgaata gatccgtttg cgtatggcca
     5761 tgcgacacag accagaatgt tttcgggcat atattaagaa ttatctccta aaaaggtgcc
     5821 atcgaatgtt agcacatggt atgtatctag gtttcaactg ctgtcaaaat tgcgatcaac
     5881 cggaactact cggtcagagc caaggcccta tctcgtaact gcccacatcc tgcatcgata
     5941 tcaatccctt tttccattct taccgttgac ggcacattga atgtttttag atccgactgg
     6001 aatctctggg cctccttgta agtggttcgg tggtatggt
//
LOCUS       DASCNS010000154         1278 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001217295, whole genome shotgun sequence.
ACCESSION   DASCNS010000154 DASCNS010000000
VERSION     DASCNS010000154.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001217295"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="QF838_05655"
     CDS             <1..330
                     /locus_tag="QF838_05655"
                     /inference="COORDINATES: protein motif:HMM:NF020039.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HJO60200.1"
                     /translation="RIPIDAEGEPGNPEIVVDMPNSVPDGLAFDSDGNLYISCYRPDS
                     IFRLDPTGTLEVLAHDYEGTLMAAPTNIAFCGDNLDDLLSANLGRWHISRYNIKASGL
                     PLNYPVI"
     gene            complement(363..>1278)
                     /locus_tag="QF838_05660"
     CDS             complement(363..>1278)
                     /locus_tag="QF838_05660"
                     /inference="COORDINATES: protein motif:HMM:NF025600.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HJO60201.1"
                     /translation="TAMVNLSPEIKFTINPNLCKDNQGPDCTELRLGDDYFTTSFPEI
                     GYLYSCNDKNPNAPGSIESELTWIDFADKTWNLLQKLWLPETASGFDQGSYAEASLGQ
                     TREITTNNLPVDGKIGDWPMTSYPTLTKIDGNPGIPASQKSSLSYPSNPEEETTPTCV
                     SFGSIGVTKNGVVIYHAADGRGEDAVAREIVDVFGGHPAGSNYHYHFIPERLDNDSLG
                     DGHSGIVGYINDGFPIYGYKGKDGIEMSNDGLDLCHGHDHGSLGYHYHATIEYPYTVG
                     CYKGTPSSAEANQVQPGGPPEGPPRRRP"
BASE COUNT          320 a          270 c          311 g          377 t
ORIGIN      
        1 cgaataccca ttgacgcaga gggcgaaccg ggtaatccgg aaattgttgt tgatatgcca
       61 aattctgttc ctgatgggtt ggcgtttgac tcagacggga acctttatat ttcatgttat
      121 agaccggaca gcatatttag gctggatcct acgggtacct tggaggtttt ggcccacgac
      181 tatgaaggca cccttatggc tgctcctaca aacatagcct tttgtgggga caatttggat
      241 gatctgttaa gtgcgaacct agggagatgg catataagta ggtacaacat taaggcttcc
      301 gggttacctt tgaattatcc ggtaatttag aagggtttca cctgtaaata aattgatttt
      361 cgttagggtc ttcttctagg gggaccttca ggaggcccac cgggttgcac ttggtttgcc
      421 tcagccgagc tgggtgtccc tttgtagcat cccacagtgt aaggatattc gatggttgcg
      481 tggtagtgat aacccaatga gccatggtca tgcccgtggc ataagtctaa accgtcattt
      541 gacatctcaa tgccgtcttt acccttatat ccataaatcg ggaaaccatc gttgatgtat
      601 ccaacaattc ctgaatgtcc atcgcctaga gagtcattat ctagacgctc aggaatgaaa
      661 tggtagtgat aattactacc tgcaggatgg cctccaaaaa catcaacgat ctccctcgcg
      721 acggcatctt cgccgcgtcc atcggctgca tggtaaataa ctaccccatt tttggtaact
      781 cctatggaac caaatgatac acaggttgga gttgtttctt cttcggggtt tgatggatat
      841 gacaaggaac ttttttggga tgctggtatt cctgggttac cgtcgatttt agtcagagtc
      901 ggataagaag tcattggcca gtcaccaatt tttccgtcaa caggtaaatt gttggtagtg
      961 atttcacggg tttgccctaa ggaagcttct gcatagctgc cttgatcaaa acctgaggct
     1021 gtttcaggga gccataactt ttgaaggagg ttccaggtct tatcagcaaa atcaatccaa
     1081 gtgagctcac tttctatgga gccgggtgca ttgggattct tgtcattgca ggaatatagg
     1141 tagcctattt ccggaaagga ggttgtaaag tagtcatctc ctagccttaa ctcagtacaa
     1201 tcgggcccct gattatcctt acataaattt ggatttatgg tgaactttat ctcaggtgaa
     1261 aggttgacca tggcagta
//
LOCUS       DASCNS010000155         1656 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001223261, whole genome shotgun sequence.
ACCESSION   DASCNS010000155 DASCNS010000000
VERSION     DASCNS010000155.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001223261"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            35..>1656
                     /gene="uvrC"
                     /locus_tag="QF838_05665"
     CDS             35..>1656
                     /gene="uvrC"
                     /locus_tag="QF838_05665"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HJO60202.1"
                     /translation="MRDENNVILYVGKASNLRNRLRSYFVPTHHEDLKTRVLVSKIED
                     FEFIVTESEQEALILECNLIKEYQPQFNSRLKDDKSYPFIKIDNSENFPQVYITRNIQ
                     KDNAQYFGPYASAGSVRKTLSLLKKLFPYRSCTKTITGTDDRACLDFHINRCIGPCIG
                     AADKNQYKDIIDQVVLFLEGSTNQIVRSLKDKMGQSAENLEFEKAAVIRDQLKSIEKV
                     HEGQKVLTLRPESLDVIACSQWSDDAWIEIFFIRQGKLIGRDSYLMEVGQYDDIQGVQ
                     NAFLQQFYEVAPYIPPTVLIQIPVGEEGSHIEAFLSAKRGSSVRLYRPVRGEKRRLVE
                     MVSNNAREALDQHKVKKFVKLNLDDTAIEELQEALNLPRLPRRIECYDISNIQGTNAV
                     GSMVVFEKARPKTSEYRRFQIRNVKGVDDYSMMKEMLTRRFKRLVDKNSSSSSWTSDP
                     DLVLIDGGKGHLGVALQVFLELGVTDIPLASLAKENEELFVPYMLEPILLPRNSPALF
                     LVQRARDEAHRFAITYHRAKRSKSTLKSTLDS"
BASE COUNT          516 a          315 c          374 g          451 t
ORIGIN      
        1 agctcatgta cccaccggtc ctggggttta tttgatgcgg gatgaaaata acgtcatcct
       61 gtatgttgga aaggccagca atcttagaaa tcgccttaga tcctattttg ttccaactca
      121 ccatgaggac ctcaagacaa gggtactggt ttcaaagatt gaagattttg aatttattgt
      181 tacagaatct gaacaggaag cattgattct tgaatgtaac ttgatcaagg aatatcagcc
      241 tcaatttaat tctagattaa aagatgacaa gtcctatcca tttatcaaaa tagataattc
      301 ggaaaatttc ccccaagttt acattacgag aaatatccag aaagacaacg cccaatactt
      361 tggcccctat gccagtgccg gaagcgtgag gaaaacattg tctcttttaa agaagctatt
      421 tccctataga tcttgtacta agaccatcac tgggacagat gacagggcat gcctagattt
      481 ccacataaac aggtgtatag ggccgtgtat cggtgcggct gataaaaacc aatacaaaga
      541 tattattgat caggtggttc tttttctaga aggtagtaca aaccagattg tcaggtccct
      601 aaaagacaag atgggtcagt cggctgaaaa tttggaattt gaaaaagccg ctgttataag
      661 ggaccagcta aagtccatcg aaaaggttca tgagggtcaa aaagttctca ctttgagacc
      721 agagagtttg gatgttatag cttgttccca atggtcagac gacgcctgga tcgagatatt
      781 ttttattcgc cagggaaaat taattggccg cgattcgtat ctcatggaag ttggacagta
      841 tgatgacatt caaggtgtac aaaatgcatt tcttcagcag ttctatgagg tcgcaccata
      901 catacctcca actgtgctaa tccaaattcc tgtgggtgag gaaggttccc atatagaagc
      961 atttctatct gcaaaaagag ggtcttcggt tcggctttat cggcctgtca ggggagagaa
     1021 aaggcgattg gtggaaatgg tttccaataa tgccagagag gcattggatc aacacaaggt
     1081 aaagaaattt gtgaaattga acctagatga cacagccatt gaagaactcc aagaggcttt
     1141 gaatttacct aggttgccta gacgaattga gtgctatgac atttccaaca tacaaggtac
     1201 caatgctgtt ggcagtatgg tggtatttga aaaggccaga cctaagacat cagagtatag
     1261 gagattccaa ataagaaacg taaaaggggt ggacgattac tctatgatga aggagatgtt
     1321 gaccaggcgt ttcaaaaggt tggttgataa aaattcatca tcctcatctt ggaccagtga
     1381 cccggatttg gttttaatag atggcggtaa aggccacctt ggggttgccc tgcaggtatt
     1441 cttagaactt ggggtgacag atataccatt ggctagttta gccaaagaaa acgaagaatt
     1501 gtttgttccc tacatgttgg aaccaatcct tttacctaga aattcccctg cactctttct
     1561 agtgcaaaga gctagagatg aggcacatag atttgccatt acataccata gggcgaaacg
     1621 ctctaaaagc acccttaaat ctacactgga ttccat
//
LOCUS       DASCNS010000156         7996 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001225352, whole genome shotgun sequence.
ACCESSION   DASCNS010000156 DASCNS010000000
VERSION     DASCNS010000156.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001225352"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            113..1135
                     /locus_tag="QF838_05670"
     CDS             113..1135
                     /locus_tag="QF838_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60203.1"
                     /translation="MSSSPIAKTYSFSTLQQASEYFLKQGWTDGLPINLPTEQEVSKF
                     IDLSGKSYKDVVGVEPVKGREITMEKVAINSVMAGCKPEYFPIVLTAVEALLESKFNL
                     HGITASTMGAGILTLVTGPIAKEIGLNGGISLFGPGHQANATIGRALRLVVINCTGSR
                     SGEIDKATLGHAGKYTWCITENEDFRPWPNTAEDRGFSKDANTVSVFAGLSPIQVSNH
                     SSQDPENILRSFRDALFAAGPNQGEIVIIFCPEHMQSFKKAGWTKNQIRDFLYEMAAR
                     TNDEWGFGSIPPGPRPTGSEITPSALTPESFTLLVGGGAAGAFSCVIPLWAGGVGSQS
                     VTKELI"
     gene            1200..1490
                     /locus_tag="QF838_05675"
     CDS             1200..1490
                     /locus_tag="QF838_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60204.1"
                     /translation="MVEGIRVLDPTIDANSQNSLLAIRPESLNGLTVGLLANGKKNSV
                     EILRYVYEILKDDHGLGAVIEDNKGNASRPCPPNLLEKLAKQCDVIITASGD"
     gene            1557..1733
                     /locus_tag="QF838_05680"
     CDS             1557..1733
                     /locus_tag="QF838_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60205.1"
                     /translation="MICTEPFVTSAVAMSKMGGIPEFPFAVVPHPLGSLDETLLRERA
                     REVAKDIVKILLEG"
     gene            complement(1748..2557)
                     /locus_tag="QF838_05685"
     CDS             complement(1748..2557)
                     /locus_tag="QF838_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HJO60206.1"
                     /translation="MPNERERLIELQKSQWQSLEKLCTSLSLDEWEVDTDCPTWTVKD
                     CIAHLVGIEHRILGREVPDMPLDEMCHIRNEQGAKNEIDVVSRRAMTPAEVLEEFSEV
                     NSERLKMLNGQLDFAAEADSPIGKGNVADQVSVRVFDCWVHEQDIRRAIGKQGNLLGL
                     AAQHSYERMTSVMPFVVEKKAGAPERSSVRFKICGESFFVVNIRVDEGRARLVKELSE
                     PTIELEMDCEVFMCLCCGRWDPDKILSDHKGLIQGDRHLGISIVKNMNYMV"
     gene            complement(2570..3046)
                     /locus_tag="QF838_05690"
     CDS             complement(2570..3046)
                     /locus_tag="QF838_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HJO60207.1"
                     /translation="MREKKYWLMKSEPSDYSFEELKSEDKATAEWDGVRNYQARNLLR
                     DEIKIGDGVLFYHSNSKPSAVVGTAVVVKDGYADFTAWDPDSKHPDPKSSPDNPIWFM
                     VDIKADLSFDTPIPLQEIKKIPALSDMVLVKNSRLSVQPVKNQEFDLIVRMGTNQD"
     gene            complement(3091..3909)
                     /locus_tag="QF838_05695"
     CDS             complement(3091..3909)
                     /locus_tag="QF838_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024762.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HJO60208.1"
                     /translation="MKLLRTIQTELNNTSVSLATLGLLVSTLEYPEINIDEELASFTQ
                     LESVLSRRIQSNSSLLQKLNEISDYFFSELRFSGNVKNYYDHKNSFINEVLNRRQGIP
                     ITLAIVYMEIASRLGIDVAGIGMPGHFLVRASDETEIFYIDVFDNGTVLSKDECEKNF
                     NDHVSAPFTWNDRYLSPVDDKYIVVRLLRNLKYIYLSEGKSKQAYKVMDLIVGLEPDN
                     VFEIRDRGMIGFRVGHQRQSIDDLKRFLKKEPVGRSAVEASSVLELLERNDKKW"
     gene            complement(3951..4048)
                     /locus_tag="QF838_05700"
     tRNA            complement(3951..4048)
                     /locus_tag="QF838_05700"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4012..4014),aa:Sec,seq:tca)
     gene            complement(4092..4421)
                     /gene="trxA"
                     /locus_tag="QF838_05705"
     CDS             complement(4092..4421)
                     /gene="trxA"
                     /locus_tag="QF838_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HJO60209.1"
                     /translation="MANTVEITDSNFQEEVLNSDIPVLIDFWADWCQPCKMIAPVVQQ
                     IADEYEGKIKVGKLDVDSNGQTSMDYNIRGIPALLIFSDGKPVDQIVGAVPKSIIQKK
                     IDEALSG"
     gene            complement(4490..5050)
                     /locus_tag="QF838_05710"
     CDS             complement(4490..5050)
                     /locus_tag="QF838_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60210.1"
                     /translation="MIIFTNFRSILAVLSACLISITFFVSCNSEKLDLGATGRNLEVH
                     ASRPQVVSKAFYTEDGITRIITPSASNRKLAVVNTTIVNRSSTVIPISVDPDAATLGD
                     RRGKKSNAIDPFTRSKEISGTLSLDPDVLEITPVLWGEIELARGFQVSGSLVFDVPKG
                     LLLGTIFWDEVEYIPVDFIDYWKDND"
     gene            complement(5110..6306)
                     /locus_tag="QF838_05715"
     CDS             complement(5110..6306)
                     /locus_tag="QF838_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HJO60211.1"
                     /translation="MPQSENGIIYFDHAATTRMRPEVLETMLPYLQFSYGNPSSIYTL
                     AQEARKAVDESRESVARILGARISEIVFTSGGTESDNAAIKGAASAMRNLGTHIITTE
                     IEHHAVLHTCQQMEQFGFDVTYLPVDSYGRVSVDEVLGSIREDTILVSVMMANNEIGT
                     IQPIKKIAEAVKLLGQEKGKTILVHSDAVQAAGSLEINVKDLGVDMLSISAHKFYGPR
                     GVGLLYLRRGTPFEPLIMGGGQERQNRSGTENVPGIVGLAKALELAVKDREELQVSLS
                     AKRDRIIRGLRDEIEGMTINGHLTDRLPNTVSVSFRGVEGEPILLGLDFAGICASSGS
                     ACSSASLEPSHVLLAIGHSAELAQGTLRITLGRDNSDGDVDYLMEVLPNLIKRLRDLP
                     SLTAHP"
     gene            6536..7504
                     /locus_tag="QF838_05720"
     CDS             6536..7504
                     /locus_tag="QF838_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJO60212.1"
                     /translation="MIDAIDITKYYGDFPAVTGLNFSVEQGEILGFLGPNGAGKSTTM
                     KILTGFIPPSSGTAKIAGYDIVEQSLLARRHIGYLPETVPLYEDMEISDYLSFMGQIR
                     GMSSSQLKKRIDEVVELCRLEEYRNTIIGKLSKGYRQRVGISQAILHEPDVLVLDEPT
                     IGIDPIQVVETRQLIKNLGEEHTLILSSHILPEVSMICDRVIIIHEGQIVAVDDPSNL
                     GTNLSGIERVEIEVKGPNREIVPVLEEIDGVQSIERIPVTGGAYHRYHITVDIGRDLR
                     HDLAKMVVASGWELLKLQSLGMSLEEIFLRLTIEEEELPSPPGEAS"
     gene            7501..>7996
                     /locus_tag="QF838_05725"
     CDS             7501..>7996
                     /locus_tag="QF838_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024110.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJO60213.1"
                     /translation="MTNLIAITRKEAKAYFSTPSAYIVGAMFLVLTGVFFVFDVSTPF
                     AEAGVRGLSDWASFFIVFLAPLITMKLLAEEQKLGTLELLLTSPVREWEVVLGKYLAS
                     LLMLLAIQSVTLYYVFMIYFFGNPDTGPVISAYLGLVLHGAAALAIGMLGSSLSGNQI
                     VSAVV"
BASE COUNT         2335 a         1814 c         1572 g         2275 t
ORIGIN      
        1 atacaatcaa ccaatagttt gattcatatt actaacctag tagtagtgtt tccgtataaa
       61 tgagtaaaat gtgttttcta tttacaaaat ttcacaaaca tggggtgaaa tcgtgtcctc
      121 atcaccaata gcaaaaacct attccttcag tacattgcag caagcgagtg aatactttct
      181 taagcagggc tggaccgatg gtcttccaat taatttaccc acagaacaag aagtttccaa
      241 atttatagac ctttcaggta aatcttataa agacgttgta ggagttgagc ctgtcaaagg
      301 cagggaaatt acaatggaga aagtcgccat aaactcagtg atggctggtt gcaaaccaga
      361 atattttccc atagtgctaa cagctgtgga ggcgctgctg gaatcaaaat ttaatctcca
      421 tgggattacc gctagtacta tgggagcagg cattttgacg cttgtgactg gacccatcgc
      481 gaaagaaata gggttaaatg gtggaataag tcttttcggc ccagggcatc aagcaaacgc
      541 gaccattggc cgggcgcttc gattagtggt aattaattgc accggatcca gatctggaga
      601 aattgataaa gccactctgg ggcatgctgg taaatatacc tggtgcataa ctgaaaatga
      661 agactttcga ccatggccca acacagcaga agacaggggt ttttcgaaag atgccaacac
      721 cgtttcagtg ttcgcaggcc tatctccgat ccaagtaagt aatcattcga gccaagatcc
      781 cgaaaatata cttagaagtt ttagagatgc cctttttgct gcaggcccta accaaggaga
      841 gatagtaata atattttgtc ctgaacatat gcaatctttt aaaaaagctg gatggaccaa
      901 gaaccagatt agagattttc tttatgagat ggccgcaagg acaaacgacg agtggggatt
      961 cggatctata ccccctggcc ccagaccaac gggctctgag ataacaccct ctgcattaac
     1021 accagagagc ttcacattac tagtgggagg aggtgcggca ggagcgtttt catgcgttat
     1081 accactttgg gcaggcgggg taggatccca atctgtcaca aaagaattaa tttaaagtca
     1141 tataaccaag tgtaaatcag aagatactgt atgatgggga aaagaaaaag ggggaactta
     1201 tggttgaagg aattagagtt ttggatccaa caatagatgc caattcacag aattccctac
     1261 tggcgatacg ccctgaatcg ctcaatggat tgacggtagg gctgcttgcc aacggcaaaa
     1321 agaactctgt agaaatactc cgatacgtgt atgagattct caaagatgat catgggctag
     1381 gggctgttat tgaagataac aaaggtaacg cttcaagacc atgccctccg aatttgctag
     1441 aaaagctagc caaacaatgt gatgtgatta tcacagcctc gggtgattga gggtcatgtt
     1501 cttcgtgcag tgtgcacgac ggaattcaat ttgagaaaaa aggagtccct gctgcagtga
     1561 tctgcacgga accatttgtg actagtgccg tagctatgtc aaaaatggga ggcatcccag
     1621 aatttccttt cgctgttgta cctcatccac tgggtagctt agacgaaact ttgttaagag
     1681 aaagggcgcg agaagtagca aaggatatcg taaaaatcct cctagaagga taagaatcag
     1741 aagatagtca aaccatataa ttcatatttt tgacgattga aataccaagg tgtcgatccc
     1801 cctggatcaa ccccttgtga tcagacagaa ttttatcggg gtcccacctc ccgcaacaaa
     1861 ggcacataaa cacctcacag tccatttcca actcaattgt tggctctgaa agttccttta
     1921 caagtcgggc cctcccttca tcaactctta tattcacaac aaaaaaagac tctccgcata
     1981 tcttgaacct tacagaactc ctttccggag cccctgcttt cttctcaacc acaaagggca
     2041 tcacactagt cattctttca tacgaatgtt gagcagcaag acctaacaaa ttaccctgct
     2101 ttccaattgc cctacgaata tcttgttcgt gtacccagca atcaaatact ctgacagaca
     2161 cttgatcagc aacatttccc ttgcctatag gtgagtcggc ctcagcagca aaatctagtt
     2221 gcccgttaag catcttcaat ctttctgaat taacctctga gaattcctcg agcacctctg
     2281 caggagtcat cgcgcgccgg gaaaccacat ctatttcatt tttcgcacct tgctcattcc
     2341 tgatatgaca catctcatcc aaaggcatat ctggcacctc tctaccgaga atacgatgct
     2401 ctatgccaac caaatgagct atgcaatcct tcaccgtcca agtggggcaa tcagtatcaa
     2461 cttcccactc atccaaactc aaagatgtac ataatttttc caaagattgc cactgggatt
     2521 tttgtaattc gatcaacctt tctctttcat tgggcatgtg tgatttaaat taatcctgat
     2581 ttgttcccat tctcacgatt aagtcaaatt cttggttttt aacaggttgg acggataatc
     2641 tggagttctt taccaacacc atatcggaaa gagctggaat cttttttatc tcctggagtg
     2701 gtataggggt atcaaacgat aaatcagctt ttatatcaac cataaaccat atcggattat
     2761 ccgggctgct tttggggtct gggtgttttg aatccggatc ccaagccgtg aaatccgcgt
     2821 atccatcctt gactactact gcggttccaa caacagcaga aggttttgaa tttgagtggt
     2881 aaaacaatac cccgtcgcca atttttatct catcccgcag gagatttcta gcttggtaat
     2941 tcctaacccc atcccattcg gctgtggcct tgtcttccga tttaagttct tcaaaggaat
     3001 aatcagacgg ttctgacttc atcagccagt atttcttttc tctcattttt tcctccgctg
     3061 gaacatcggg atgaattata cgtacatccc ctaccatttc ttgtcattcc tttccagaag
     3121 ttctaataca ctggaggctt caactgctga acgtcccaca ggctcctttt tcaaaaatct
     3181 tttgagatca tctatagatt gtctttgatg tcccacccta aaccctatca tccctctatc
     3241 cctaatctca aaaacgttgt ccggctcaag accaactatt aaatccatga ccttgtaggc
     3301 ttgtttactc ttaccttcac ttaagtaaat gtatttcaaa tttcgcaaca gtcttacaac
     3361 tatgtatttg tcatctactg gcgacaaata acggtcattc caagtgaaag gggccgagac
     3421 atgatcatta aagtttttct cacattcatc tttacttaac acggtcccgt tatcgaatac
     3481 gtctatgtaa aatatttcgg tttcatcact agcacgaaca aggaagtgtc ctggcatccc
     3541 gatgcctgca acatcgatcc ccaatctaga ggcaatctcc atgtaaacta tagcgagggt
     3601 aattggtatc ccttgacgcc tgtttaaaac ttcatttatg aaactatttt tgtgatcgta
     3661 ataattctta acattccctg agaaacgtag ttctgaaaaa aaataatcac tgatttcatt
     3721 taatttttgc aaaaggctag agtttgactg aattctccga gacaacactg attccaattg
     3781 ggtgaatgag gccaattcct catcaatgtt gatttctggg tattccaagg tggagactaa
     3841 tagccctaaa gtagccaggc tcaccgaagt attatttaac tctgtttgta ttgtgcgaag
     3901 taatttcaat cctgcaccca taggttatct gagactatac atcagccata tggcgggagc
     3961 gcgagggagt cgaacccacc gcaccaatcg aaggattact gccaacggag ttgaagtccg
     4021 cagagaccac caggccccat ccgctcccag attaagttgt cctctattac tgaataggta
     4081 atttaataaa attaccccga aagggcctca tcaatcttct tttgaattat ggatttcgga
     4141 acagcgccca ctatttggtc aactggcttc ccgtcactaa atatcaaaag agctggtatt
     4201 cctctgatgt tgtagtccat agaagtttgc ccattactat caacgtctaa tttgcctact
     4261 ttgattttgc cttcatactc atcagcgatc tgctgaacta caggagctat catcttgcat
     4321 ggctgacacc agtcagccca aaaatcaata agcacaggta tatcagagtt tagtacttct
     4381 tcttgaaaat tactgtccgt tatttcaaca gtgttggcca taacatattc tccttgctac
     4441 ctaaagttgt tctttaattt actatatctg gcttgtctgt aatttgacat taatcgttat
     4501 ctttccagta gtcgatgaaa tctacaggta tatattcaac ttcatcccaa aatattgtac
     4561 ctaaaagcag tccttttggc acatcaaaaa ccaaactacc agatacctga aagcctcttg
     4621 ctagctctat ttccccccac aaaaccggtg ttatttctaa aacatcagga tctaggctca
     4681 aggtccctga aatctccttg gaacgtgtaa aaggatctat tgcattagat ttctttcctc
     4741 tccggtcacc gagtgtcgca gcatctgggt ctacagatat cggtataacc gtactacttc
     4801 tattgactat agtcgtgttg acaacggcca atttcctatt cgatgcacta ggtgtgataa
     4861 tccttgtgat tccatcttcg gtataaaacg ccttggaaac aacttgaggt cgtgaggcgt
     4921 gtacttccaa gttcctacca gttgctccca aatctaattt ctccgagtta caagagacaa
     4981 aaaaagtaat tgaaattaaa caggccgata aaacagctaa aatcgatcga aaattagtaa
     5041 aaataatcat ggtttctcta aatgttttaa ttcttaaaat acaggctcaa caatctatca
     5101 catcgtaaat caaggatggg cagttaagga aggcaaatca cgtagccttt ttatgagatt
     5161 cggaagcacc tccatgagat aatctacatc tccgtcagag ttatcccgac ccaaagttat
     5221 tcgaagggta ccctgagcta actccgcaga atgacctatc gctagcaaaa catgcgatgg
     5281 ctccaatgaa gctgatgagc atgcacttcc actagaagca caaattccgg caaaatctaa
     5341 acctagaaga ataggctctc cctcgactcc gcggaaagat acactaacag tattcggaag
     5401 ccggtctgtt aaatggccgt ttatagtcat tccttctatt tcatctctta gtcctctgat
     5461 tatcctgtca cgcttggcag ataagctgac ctgtagttct tccctatcct ttacagctag
     5521 ctctaaagcc tttgctaacc ccactattcc gggaacgttc tcagtgccag atcggttttg
     5581 gcgttcctga ccacctccca ttatcaaagg ttcaaatggg gtacctctcc tcaaataaag
     5641 aagccctacc cctcgtgggc cataaaattt atgggcagaa atacttagca tatctacccc
     5701 taaatctttt acatttatct cgagtgaacc agccgcctgc actgcatcac tgtgaaccaa
     5761 tatagttttt cctttttcct ggcctaataa tttgacagct tctgcaatct tttttatagg
     5821 ctgaatagtc ccgatttcat tgtttgccat catcacactg acaagaatag tgtcctctct
     5881 tatagagcct aaaacctcat ctacagaaac cctcccatag gaatcaacag gcaaataagt
     5941 cacatcaaac ccaaactgtt ccatctgttg acaagtatgc aaaacagcat gatgctcaat
     6001 ttccgtcgtt ataatatggg ttcccaaatt acgcattgcc gaggcagcgc ccttaattgc
     6061 agcattatca gactcggtcc ctccactagt aaatactatt tcactaatcc tagcgccaag
     6121 tatccgtgcc actgattctc gagattcatc aaccgctttc ctcgcttcct gagctagggt
     6181 atatatgctg gaagggttcc catagctgaa ttgcaaatat ggcaacatcg tctccaacac
     6241 ctcgggcctc atgcgcgtag tcgcggcgtg atcaaaatag atgattccgt tttctgactg
     6301 tggcatgacg atcaatcctc cctcgcaatc acaaccaaat gtccctctat agatcttagc
     6361 atgtccattt gttacagata ggtctgatag aaaggtgaaa atatcattgt aattgtgaag
     6421 gagtttaaaa tatgcttgta cactggaacc ggttagatta acattcctca gttctctttt
     6481 taccgtcgac caataccgga taaaatgtga aacgcaggaa ttaaggatta cataattgat
     6541 cgatgcgata gacataacta aatattacgg tgacttccct gctgtaacag gcttgaattt
     6601 ttcagttgaa cagggagaga tccttggttt tcttggacct aacggggccg gtaaatcaac
     6661 aacaatgaaa atattaaccg gatttatccc tccttcttcc ggtactgcca aaattgccgg
     6721 atatgacatt gttgaacaat ccctacttgc tagaagacac atcggatatt taccagagac
     6781 agtgccacta tacgaggaca tggagatcag tgattacctg tccttcatgg gtcaaatcag
     6841 gggaatgtct tcctctcaat taaaaaaacg gatagatgag gtggtcgaac tgtgccggct
     6901 tgaagagtac agaaatacta ttattggcaa actctctaaa ggatatagac aaagagtcgg
     6961 aatatcacaa gcaatactgc atgaacctga cgttttagta ttggacgaac ctactatagg
     7021 aattgatccc atccaagtag tagagactag gcaattaata aaaaacctag gcgaagaaca
     7081 taccttgata ttaagttcgc acattcttcc ggaagtgagc atgatctgtg acagggtcat
     7141 catcattcat gaagggcaga tcgtggctgt tgacgatcct agcaacttag gtactaattt
     7201 atcaggaata gaacgggtag aaattgaggt aaaagggcct aatagggaaa tagttcctgt
     7261 gctcgaagag atagacggag tccaatcaat agaaagaatc ccagtaacag gtggcgccta
     7321 ccacagatat catattacgg tagatatagg acgagatctt agacacgact tagccaaaat
     7381 ggttgtagct agtggttggg aacttttgaa attacagtca cttggtatgt ctttagaaga
     7441 aatattcctg agacttacaa tagaggaaga ggaattaccg agtcctccag gggaagcttc
     7501 ttgacaaatt taatcgctat tacccgaaag gaggctaaag cctatttttc aactccctct
     7561 gcctacatag tgggagcgat gttcttggtt ttgaccggtg tgtttttcgt atttgatgta
     7621 agcacgccat ttgccgaagc tggagtgaga gggctttctg attgggcaag tttcttcatc
     7681 gtgttccttg cacctcttat cacaatgaaa ctattggctg aggaacaaaa gttaggtacc
     7741 ttggagttgt tactaacctc cccagttcgg gaatgggaag tagttttagg aaaatatctc
     7801 gctagccttt tgatgctttt agcaatccaa tcagtaacct tgtactacgt ttttatgatc
     7861 tacttttttg gaaatccaga tacaggccca gtcatcagtg cctatcttgg actggtacta
     7921 cacggggcgg cggctctggc aataggtatg ctaggttcat ccctttcagg taaccaaata
     7981 gtatcagctg tagttg
//
LOCUS       DASCNS010000157         7202 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001231893, whole genome shotgun sequence.
ACCESSION   DASCNS010000157 DASCNS010000000
VERSION     DASCNS010000157.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001231893"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="QF838_05730"
     CDS             complement(<1..939)
                     /locus_tag="QF838_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012673.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HJO60214.1"
                     /translation="MNIPLEKDFIVNLQNDAYELAMSAGRLLQMYFRDPLSESQDLKV
                     SYKDSKGRNPVTMADKDVQDFLSEEIIKRYPGHGIVGEESDSTEDSIPDIVWVLDPLD
                     GTRNFMHGFPCYASSIGIMYKGFPIAGAIFIPWPNQNPGIVIHASRNNGCKINNKPLE
                     TPVIKEIDPNAINTIPGSFAGRFKFDSQFSSKSGESRMGGSIAYEMSLVALGITEYAV
                     ISGSHLWDVLAGIVLVNESGGSSLMQNAGNSLKSDTGVWSEFGAVVENWESGKTKVSD
                     LRRGVSPLLLAQSDIINTLAANIQLKENPIKKIAKSI"
     gene            complement(970..1950)
                     /gene="meaB"
                     /locus_tag="QF838_05735"
     CDS             complement(970..1950)
                     /gene="meaB"
                     /locus_tag="QF838_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HJO60215.1"
                     /translation="MSNYDKPQEQIIRLLDGNMRALSKLITLVEPDPQILASVYPVIK
                     DVGGNSHIVGITGPPGAGKSTLSSALASIIRDTGRTVAILACDPSSPFTGGSVLGDRV
                     RMQSHYTDPGVFIRSISSRGSGGGLSTSTYGITTLLDAFGFDVVLLETVGVGQTELDV
                     MKISDTVIVVIVPESGDSIQAMKAGLMEIADIFVVNKSDRVGADRMVKDVEGALRLSD
                     SHAVNLPSVLKIRADAGDGVPELLLEIDRIYSDRGYCDRLAVHKSTKRLEALRTTTLL
                     EIESKLDMILDGENSSTNSEVAKLKKDVELGRIDPISASRILVDLIKNLE"
     gene            complement(2017..2505)
                     /gene="ribH"
                     /locus_tag="QF838_05740"
     CDS             complement(2017..2505)
                     /gene="ribH"
                     /locus_tag="QF838_05740"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HJO60216.1"
                     /translation="MPRGIDPVIEGDLDGTGMRFGIVVAEFNDFITLKLLDGAKSALL
                     EHGVSESDITVAKVPGSFEIPVVADGMASSGNHDAVICLGAVIKGETDHYEYVSQGAA
                     EGISRVALDNQIPVMFGVLTTHNVEQALDRCGGRKGNEGESCATSAIRTVNVLNQINN
                     TA"
     gene            complement(2477..3709)
                     /locus_tag="QF838_05745"
     CDS             complement(2477..3709)
                     /locus_tag="QF838_05745"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HJO60217.1"
                     /translation="MPLSKIEEAIDDIRNGKMVIVIDDEDRENEGDLTMAAEMVTPEA
                     INFMATYGRGLICVPMRGEQLDKLNLPLMVSNNTANLSTAFTVSVDSLIGTTTGISAS
                     DRSNTIKALVNDETKPEDLGRPGHIFPLRYVDGGVLKRTGQTEASIDLCRLAGLREAA
                     VICEIMADDGHMARMPQLEEFAQQHDVKIVSVEDLISYRRRHERLIERVAEARVPTEY
                     GEFTAVSFSSVVDVQEHVAFVKGDVCQNDPVLVRVHSECLTGDVFGSKRCDCGYQVQL
                     ALEAIEKYGSGVLLYMRQEGRGIGINNKLKAYALQDEGYDTVEANVMLGFAPDPRQYG
                     IGAQILEDLGVKKMRLLTNNPTKRVGLEGFGLEVVERIPIVAPTTDENVRYMETKRER
                     MGHILDPIEDASGDRSGD"
     gene            complement(3735..4334)
                     /locus_tag="QF838_05750"
     CDS             complement(3735..4334)
                     /locus_tag="QF838_05750"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HJO60218.1"
                     /translation="MFTGLVEELGVISSISQTDIWIESSIIMDDLGVKDSVSVNGACL
                     TVVSIEEKSFKVNVVPETLRRTDLGDLSVGDKVNLERSALLGGRLGGHIVQGHVDGTA
                     RITAYVEEGSAWLIEFQISKKLSRYIVEKGFVCVDGASLTVVNCDQNTFTVALIPYTR
                     DNTVLGYKEVGSSVNIETDIIGKYIEQLSTGNKASIDLN"
     gene            complement(4334..5413)
                     /gene="ribD"
                     /locus_tag="QF838_05755"
     CDS             complement(4334..5413)
                     /gene="ribD"
                     /locus_tag="QF838_05755"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HJO60219.1"
                     /translation="MSYMDIALTVGKQARGISSPNPPVGAVLVKNEQVISEGFTGRPG
                     SPHAEVMAITSAGDGVKGSVLYVTLEPCCHFGRTPPCTEAIIESEVSKVYIAIKDPDI
                     RVSGKGIDRLQKAGIEVVLGVGKKEAEVDLEAHIKLSKTGIPLITAKFAVSLDGKIAT
                     SSGDSKWITSEESRTRAHFMRLESDAIMVGVNTVIADDPRLTVRLDDKKIERQPLRIV
                     IDSSGRIPEDSALFLEKGATFIASTRDFKVPDNLQNVTTKTFPSRAARVDLAAVLKHL
                     GNIPVSSVLVEGGSELMGSLFDEGLVDKVAAFISPSIIGGKSSLNAVGGRGANVMSEK
                     YSLINVSREFIGPDVLISGYCGKGD"
     gene            complement(5417..5986)
                     /gene="dcd"
                     /locus_tag="QF838_05760"
     CDS             complement(5417..5986)
                     /gene="dcd"
                     /locus_tag="QF838_05760"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HJO60220.1"
                     /translation="MVLSDRTIREEIAAGRIVVEPLGENAIQPASIDVHLDKTFLVFR
                     NSRLPYIDVRQSAEDLTEKLEIEGDTPFMLHPGEFVLGSTLEYIELPDDLVSRVEGKS
                     SLGRLGLLIHSTAGYVDPGFKGHLTLELSNVSNLPITLYYGMKIGQLSYVRLTTSAEY
                     PYGSPELGSKYQGQSDPTASKVHQDFQRG"
     gene            complement(6072..6926)
                     /locus_tag="QF838_05765"
     CDS             complement(6072..6926)
                     /locus_tag="QF838_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016300.3"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HJO60221.1"
                     /translation="MNKLIVSALLLIITGVACTSNDPITNELVEALEPTALSPVYAAS
                     STFADGGTVKGVANSDSLSIDGIQGGIRVSGTGSISVEPDVAVLEIGVEVFAGKVSKA
                     RSEASIAMESVTSTIKKLGVEDKDIQTTRFSIYPRYDYEETTMNGNRIGTQVLTGYTV
                     SNTVKAKVFEVDKVGKIIDDAAGAGGDYVRINGVDFTVDDPNPYKTTIRMMAVEDAVN
                     KAQEYALLTNVELGPIISLDEMGVGSIQSPYEADYGMRMMAAAPTTSISSGQLEISLS
                     VNTLFAIK"
BASE COUNT         2007 a         1802 c         1267 g         2126 t
ORIGIN      
        1 tatagatttc gcgatctttt ttatcgggtt ttctttaagt tgtatattgg ctgccaaagt
       61 attgatgata tctgactgcg ccagaagtag tggagacacc ccgcgcctaa ggtcactgac
      121 ttttgttttg cctgattccc aattctcaac aactgcgcca aactcggacc aaacaccagt
      181 atcgctttta agagagttgc cggcattctg catcaatgaa ctacccccgg attcgttcac
      241 cagaactatt cctgctaaaa catcccacaa atgcgaccct gatataacag cgtactctgt
      301 tattccaaga gccactaagg acatttcata agctatgctt cctcccatac gagattcccc
      361 tgatttagaa gaaaactgac tatcaaattt gaacctaccg gcaaaggaac caggaatggt
      421 atttattgcg tttggatcaa tttctttaat tacgggggtt tcaagcggtt tgttgtttat
      481 tttgcaacca ttatttcggc tcgcatgaat aacaataccc ggattctggt tgggccaggg
      541 tataaatatt gcaccagcaa ttgggaaccc tttgtacata attccaatag aagatgcata
      601 gcaggggaag ccgtgcataa aattacgggt accatccaaa gggtctagca cccaaacaat
      661 gtcaggaata gagtcttcag ttgaatcact ctcttcccca acaatcccat gccctggata
      721 ccttttaatt atctcttcag agagaaaatc ttgtacatct ttatctgcca tagtcacggg
      781 gtttcttcct ttcgaatcct tgtaacttac cttcaaatct tgtgactctg atagagggtc
      841 tcgaaaatac atttggagca accggccagc tgacatagcc aattcatagg cgtcgttttg
      901 aaggtttact ataaaatctt tttccagcgg aatattcata tctaaaaggt gtttacttta
      961 tctaaaattt cattcaaggt tctttatcaa gtccactaaa atccgggaag cactaatagg
     1021 gtcgattcgc ccaagctcca cgtctttttt tagttttgcc acttctgagt tagtcgaaga
     1081 attttcacca tcaagaatca tgtctaattt agactctatc tccagcaagg tagtggtccg
     1141 caacgcctcc aatcttttcg ttgacttatg aacagcgagg cgatcacagt acccgcgatc
     1201 tgaatatata cggtcaatct ctagaagaag ttctggtaca ccatctcctg catcggctct
     1261 aattttgagg actgatggta gattgacagc gtgactgtcg gaaagccgta gggcaccttc
     1321 aacgtccttg accattctat cagcgccgac cctatcggat ttattgacaa caaatatatc
     1381 tgctatctcc atcaatccag ctttcatcgc ttgtatggaa tctccggatt caggaacgat
     1441 tacaacaata accgtgtctg aaatcttcat cacatccaat tcagtttgtc ccacccctac
     1501 agtttctaac aaaaccacat caaaaccaaa tgcgtcaagc aaggtagtaa tcccataggt
     1561 tgaagtggac aacccgccac ccgatcctct actacttatg cttctgataa acactcctgg
     1621 atcagtgtaa tgtgattgca ttcttactct atcaccaaga acagagcctc cggtaaacgg
     1681 gctacttggg tcacatgcca gtattgcaac tgtccgcccc gtgtctctaa taatcgaagc
     1741 caaagcagat gaaagagtgc tcttacctgc tcccgggggg cctgttatcc cgacaatatg
     1801 actgttccca ccaacatcct ttattacagg ataaacggag gctagtatct gtgggtctgg
     1861 ttcaactagt gtgatcaatt tagaaagcgc tctcatattg ccgtcaagca accgaatgat
     1921 ctgttcttgg ggcttatcat aattagacat attatctaac ttccatatca ttaaaagggg
     1981 ctttcaaagg aaataatcga aaatattgcg gatatctcag gcagtattat ttatctggtt
     2041 aagcacattt actgttctga tcgctgaggt cgcacaactt tccccctcat tacccttgcg
     2101 accgccacac ctatccaagg cttgttctac gttatgggtc gtcaaaaccc caaacattac
     2161 gggaatctga ttatctaaag ccacccggga aataccttct gccgcccctt gtgaaacata
     2221 ttcatagtga tcggtttcac ctttaattac tgctcctaga catataacag catcatgatt
     2281 tcctgaggat gccataccgt cggccacaac gggaatctca aaggatccgg gtaccttagc
     2341 aacggttata tcactttccg ataccccatg ttctaacaaa gccgatttgg ccccatccaa
     2401 taattttagt gtgatgaaat cattaaattc ggcaacaaca ataccgaacc gcataccagt
     2461 tccatccaag tcaccctcaa tcaccggatc gatcccccga ggcatcttcg atagggtcta
     2521 aaatatgccc catcctttct ctttttgtct ccatatacct tacattttca tcagtagtag
     2581 gagctactat cggtatccgc tcaacaactt ccaagccaaa tccttccaat ccaacccttt
     2641 tagtggggtt gtttgtcaaa agcctcattt ttttcacgcc aagatcctcc aaaatttggg
     2701 cccctatccc atactgccta ggatctggtg cgaagcccaa cattacatta gcctccacag
     2761 tgtcgtaccc ctcgtcttgc agggcgtagg ctttcaattt attgttaatc cctattcccc
     2821 tcccttcttg tcgcatgtaa agaagcactc cactcccata tttttcaatt gcctcaagag
     2881 ctagttgaac ttggtatccg caatcacacc ttttacttcc gaacacatca ccagtcaaac
     2941 attcactatg aaccctaact aatactggat cattttgaca aacatctcct ttaacaaaag
     3001 cgacatgctc ctgcacatca accacactgc taaaggagac tgctgtaaat tccccatact
     3061 ctgttggcac tcttgcttct gctacccttt cgattaatct ctcatgccgc cgccgataag
     3121 agatcaaatc ttctacgctg acaattttta catcatgctg ttgagcaaac tcttctaatt
     3181 gaggcatcct tgccatatgg ccatcatctg ccattatttc gcaaatcaca gcagcttctc
     3241 ttagcccagc taacctgcaa agatctatag aagcttcagt ctgtccagtc cgttttaaaa
     3301 ccccaccatc aacataccgc agaggaaaaa tatggccagg tcggcccaaa tcttctggtt
     3361 ttgtttcatc attcacaaga gctttaatag tattagatct atccgaggca gaaattccgg
     3421 tggtagttcc tatcaaggaa tcaacagata cagtaaaggc agtacttaaa tttgcagtgt
     3481 tgttggagac cataagcggc aaatttaatt tatctaattg ttctccacgc attggcacgc
     3541 atattaaacc ccgaccatat gtagccataa aattaatagc ttctggagtc accatttccg
     3601 ccgccatcgt taggtcgcct tcattctcac gatcttcatc atcaataacg atgaccattt
     3661 tgccgtttct tatatcatct atggcttctt ctatcttgga taatggcatg gatatttatc
     3721 tttcccatat aggatcaatt gagatcgata ctggctttat tcccagttga gagttgttct
     3781 atatattttc ctattatatc ggtctctata tttacagagc tgcctacctc tttgtacccc
     3841 aaaaccgtat tgtcccgagt gtagggtatc aaagctacgg taaatgtatt ctgatcacag
     3901 tttacaaccg tgaggctagc accgtccacg catacaaacc ctttttcaac tatatatcta
     3961 gataattttt ttgatatttg aaattctata agccatgcac ttccctcttc aacatatgca
     4021 gtgattctag ctgttccatc cacatgccct tgaacaatgt gtcctccaag gcgccctccg
     4081 agtagggctg atctttccaa attaaccttg tctcctactg aaagatctcc caaatcagta
     4141 cggcgtagag tttcaggtac tacattcacc ttaaatgatt tttcttcaat gctgactact
     4201 gtaagacaag caccattcac agaaacactg tccttaaccc ctagatcatc cataatgatc
     4261 gaggattcta tccatatatc agtctggctg atagatgaaa taactccaag ttcttctact
     4321 agacctgtaa acatcaatct ccttttccac agtatcctga tatcaaaaca tccggcccga
     4381 tgaattctct cgagacatta attagtgagt atttttctga catgacattt gctcctcttc
     4441 ctcccactgc atttaggctc gatttccctc cgataatact aggagaaata aaagccgcca
     4501 ccttatccac taaaccttca tcaaataaag aacccatcaa ttccgacccc ccttctacca
     4561 ataccgagga aacagggata ttccctaagt gttttaaaac cgctgcaaga tctactcttg
     4621 cagccctact gggaaaggtc ttggttgtga cattttgcag attatcgggt actttaaagt
     4681 ctcgggttga agcaatgaaa gtagctcctt tttccaaaaa taatgcacta tcctcaggta
     4741 tacgcccaga cgaatcaatt actattctca agggctgtct ctcgattttt ttatcatcca
     4801 acctaactgt caaccgagga tcatcagcta taacagtatt cactcccacc ataatggcgt
     4861 cagactcaag gcgcataaaa tgagctcgcg tcctagattc ttcgctagtt atccactttg
     4921 agtctcctga agaggttgct atcttgccat ctaaactaac agcaaatttt gcagtaatta
     4981 aagggatccc tgttttcgac agtttaatgt gggcctccaa atcaacctca gcctcttttt
     5041 taccgacacc caaaacaact tcgatacctg ctttttgcag tcgatcaata cctttgcctg
     5101 aaaccctgat atcagggtct ttaatggcaa tatagacctt agaaacctca gattctataa
     5161 ttgcttcagt acaaggagga gttctaccga aatggcaaca aggctccaaa gtaacatata
     5221 aaacagaccc ttttacacca tctcctgctg aagtgatggc catgacttca gcatgggggg
     5281 acccgggtcg ccccgtaaat ccttctgata ttacctgttc gttttttacc aggacagccc
     5341 caacaggggg gttagggctg gatatacctc gagcctgttt tccaacagtt agcgcaatgt
     5401 ccatatatga catgaatcac cctctttgga aatcctgatg tactttgctg gcagtcggat
     5461 ctgattgccc ttgatatttg ctgcctaact ctggcgaacc ataaggatat tcagctgaag
     5521 tggttagccg cacataagac aattgcccta ttttcatgcc gtaatacaag gttatcggta
     5581 gattagatac attactcagc tcaagtgtca gatgtccttt aaaaccagga tctacgtagc
     5641 ctgccgtgga atgtatcaat aaccccaatc tgccaaggga actttttcct tccacccttg
     5701 acacaaggtc gtcaggcaat tctatatatt caagggtact gcctaataca aattctcccg
     5761 gatgaagcat aaatggtgta tcgccttcga tctccaattt ttctgtcaaa tcctcagcac
     5821 tctgccgaac atctatataa ggcaatctag aatttcgaaa aacgagaaaa gttttgtcaa
     5881 gatgaacgtc aatacttgca ggctgaatcg cattttcccc cagaggttca acaactattc
     5941 tgccagcagc aatctcctcc cttatggttc ggtcactaag taccattgtt ggtctccgta
     6001 aaattttatt ctgattctat caatagcttg aggacatacc caaaaaagtc catacagagt
     6061 cacggcagat atcacttaat agcaaacagg gtattcacac tcagactaat ctccaattgg
     6121 ccgctactta tggaggtagt cggtgctgca gccatcatcc gcataccgta atcagcctcg
     6181 tatggagatt gaatagagcc aactcccatc tcatctaatg agattatggg gcccaattcg
     6241 acattggtca aaagcgcgta ttcttgagct ttattgactg catcttccac tgccatcatt
     6301 ctaatcgtgg tcttgtacgg attagggtca tcaacggtga agtcaacacc attaatccga
     6361 acataatcac ctcccgcacc cgctgcatca tcaattattt tgcccacttt atctacttca
     6421 aaaactttag cttttacagt gttacttacc gtgtagccag taagcacttg ggtaccaatc
     6481 cggtttccgt tcattgtcgt ttcctcgtaa tcatatctag ggtagatact aaatctagtt
     6541 gtctgtatat ccttgtcttc aacacctagc ttctttatgg ttgaggttac tgattccata
     6601 gcaatagaag cttctgacct ggccttacta acttttcctg caaatacttc aactccaatt
     6661 tccagtacag caacatcggg ctccacagat atcgatcctg tacctgaaac tcttatcccg
     6721 ccttgtatcc catcaatgct taaagaatct gaattagcaa caccttttac tgttcctcca
     6781 tcggcaaatg tggaggaggc agcataaaca ggcgacaagg ccgttggctc tagggcttca
     6841 acaagctcat tggtaattgg atcattactg gtgcaagcca cacccgtaat gattaacagt
     6901 agcgccgaaa caattaactt attcatctgg tcacctattg gtagtacatt taggacaaat
     6961 atcagtctct taaaggatac aactatccgg attgaaaaac ctttcattgt gacaggtctc
     7021 ttgtactaga agatataatt agatgcaatg tacatttatg agagaaaaat tcaacgggca
     7081 gttcgcgaaa ttgttgaaac atcgatattg gctatgtttg tgttttctgt tttgtatatt
     7141 tctatccaaa attaccaggt cgaaggttcc agcatggctc caaacataaa tcaaggagat
     7201 tg
//
LOCUS       DASCNS010000158         6932 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001252215, whole genome shotgun sequence.
ACCESSION   DASCNS010000158 DASCNS010000000
VERSION     DASCNS010000158.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001252215"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="QF838_05770"
     CDS             <1..1378
                     /locus_tag="QF838_05770"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJO60222.1"
                     /translation="SEHFASQPEILRYLQHVTRKFDLIKDIQFSSDVKSAIYDDPNRS
                     WEITLGDGSKYRSRFLIAGIGLLSQPTMPKIEGIDSFEGPSYHTSRWPHQPVDFSKKR
                     VAIIGTGASGVQAIQEICKNVGQLTVFQRRPNWCAPLHNDPIGPEEMKRIRSGYDEMF
                     ERCDQTQAGFLHTVDPRGTFEVTEKERFEFWEHLYASKGFGIWQGNFKDVLSDPEANK
                     LMSDFVADKIRTRVNDPEIAEKLIPKDHGFGTRRVPLETEYFESYNRENVKLIDITEN
                     PILRITPTGILTEREELEFDFIIYATGFNAILGSYDRIGIQGTQEKKLRDQWKQELSS
                     FLGLQVNNFPNLFMILGPHALLGNNPRSIEYNVEWITDLIQHMGEKGFTRAEATAEAV
                     ASWYEYVLEQGEGLLSNEVDSWMTGVNSNLEGRQKRIIARYSGSQESYRSRCDEVAAN
                     GYVELNLL"
     gene            1495..2265
                     /locus_tag="QF838_05775"
     CDS             1495..2265
                     /locus_tag="QF838_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO60223.1"
                     /translation="MQINLTKKKLKSGAPAFGAIIHEYSPGTVELFGAIGYDFVMIDC
                     EHGGMSVDQVENMVRGAEVFGITPIARIPNHDASTILRFLDRGVQGIIVPHINTRSQA
                     EGVTKAARYHPEGDRGVGGGRAHDYGINTTRSESTKFVNDNILVIPMIEEVEAIKNVS
                     EIASVEGVDVLHVAPGDLSQSMSNPPQSEVRETMDKVVPKIRGAGKWVGVGGNSPNDV
                     ERVSDLINLGANMITIAAQGLMKSGAESFRKSVENHLT"
     gene            2304..3680
                     /locus_tag="QF838_05780"
     CDS             2304..3680
                     /locus_tag="QF838_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60224.1"
                     /translation="MRYLIRNSTVVTGNINRLILYNSSIFIQNGVIQDIGESDLLEAK
                     YPSVTIIEGVGKVIFPGLINCHTHLLATADKGILEDFGFPTTLKFPTTGRGLLNEDER
                     NVFARLACIEAIKSGTTTLLEISNDIAGYAQTLDSAGLRVFLGETLSDIDDSKSTEGV
                     YEYSSHKRDIGIGKASDLINAWDGKSNDRIRGFVAPHAPERVSPDLLRDSRDLAEKHD
                     LGYTIHLSQSYKEIEATKRIRGVLPTHYLFANEFLSNRLVAAHCRYLDSSEIAMLGQN
                     NAGISHNSAIGARRGAAAPVHELLSSGCSIGMGSDNMAEDMVEVMRAALFHERVRRND
                     EMNPQPEDILEWGTMGGARTLGIVDEVGSIEIGKKADLFMIDFRKAHLVPTLRIVSSF
                     IHNGLSSDVTDVMIDGEWVMQNSSIKTINEPEVIEQAEEIGHRVWNRLVRENPNVPFP
                     ISLPPGPL"
     gene            complement(3693..4532)
                     /locus_tag="QF838_05785"
     CDS             complement(3693..4532)
                     /locus_tag="QF838_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HJO60225.1"
                     /translation="MSNNFDKAVRAKLGVYEDQLDPIQMSKITTLQIEEATQGDIDDL
                     VKTPSVIKLSLVCKGDVSLAPLQELALLDSLRISHANLENVSPLEKLTDLRHLDVSDS
                     QIADFSHLSYLRELFSLRLKNCDLDDLSPLEGMGNLSELNLNENNIEQLNSISSLNEL
                     KSLDIESNEISNLDPLSFINGVTWIHAARNYIQDLGPLTTLKALESLDVGHNRVTNID
                     ALSKITTINWLGLSFNNIRDISPINSLPNLRYLDIEGDSFNQQSTRIHLPNLAAKGID
                     IFR"
     gene            4726..5979
                     /locus_tag="QF838_05790"
     CDS             4726..5979
                     /locus_tag="QF838_05790"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60226.1"
                     /translation="MVIDNSIPEINGPLTGIKVLDWTIWQFGPVSTSMMGDMGADVIK
                     IESLDGDPSRGLSRASSMKMDLGEGRIAYFETCNRNKRSIAVNLKTKEGQQIVYDLCK
                     DADVFVQNFRSGVAERLGVGYETLKEINPQLIYGSANGYGPHGPDAGQPSFDGCGQAR
                     SGLMMSASQGGQEYPTQVTQGVSDQIGGIMLCQGILAALVSRSIRGIGQKVETSHLSA
                     NMWLQGLGVSMSLLQGGVGFSPYNRKEPVNPLSNVYRTSDGRFIMMMHLQPDQYWEPL
                     LEAMDLQEFIGDPRYSDMPSRSQNSAEVVALLDKRFDEKTAEEWDILFRNFSTDFIYS
                     LVQTVQDLPYDPQVIANDFITNFNHPVLGEVQMCNHPSIYSETPAGIWKEAPEVGQHT
                     EQVLVDELGYSWDDIAKLQSAGTIL"
     gene            6003..>6932
                     /locus_tag="QF838_05795"
     CDS             6003..>6932
                     /locus_tag="QF838_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60227.1"
                     /translation="MGQIDGGQLFAKALKKEGVEYVFTLNGGHIYNLYEGCADEGIEL
                     IDFRHEQVAAHAAEGWAKVTGKPGVCIVTAGPGVTDAVTGIANAFQAPSPMIMVGGNA
                     GIFDHLRGGLQDFDSATFLKPVAKFSEQVKRVERIPEYVSNAFRAATSGIPGPAYLEI
                     PIDIVNGVTEEENVKYPDRYRTESRAFGDPEYIQKVTDILKNAERPMVLAGSDIWWCD
                     ASAQLREFVEKINAPVFLNAMGRGSLHSDHPNLGSLGRRYGLVKSDAVVLIGTPIDFR
                     LSYGEDRLFPQNPKLIEIMMDANKIGQNRAVDVG"
BASE COUNT         2140 a         1339 c         1627 g         1826 t
ORIGIN      
        1 gtcggagcat tttgcttcac agccggaaat tcttagatat ctacaacacg tgacgaggaa
       61 attcgatctc ataaaagata ttcaattttc aagtgatgtt aagtcagcta tttatgatga
      121 tccaaaccgg agttgggaaa tcactttggg tgatggaagt aagtacagaa gccgtttttt
      181 aattgcaggg ataggtctac tttctcagcc tacaatgcca aaaatcgaag gtatcgattc
      241 ctttgaaggc ccttcatacc atacatcaag atggcctcat cagcccgtgg atttctctaa
      301 aaagagggtg gcaatcattg gaaccggtgc aagtggagtc caagcaatcc aggagatctg
      361 caaaaacgtg gggcagctga cagtatttca acggcgcccc aattggtgtg cacccttaca
      421 caacgaccca atcggtcctg aagaaatgaa gaggatcaga tctggttacg atgagatgtt
      481 cgaacgatgc gatcaaacac aggcaggttt tctccacacc gttgatccta gaggcacctt
      541 cgaagtaacg gaaaaagaga gatttgaatt ctgggaacat ttgtatgcaa gtaaagggtt
      601 cggtatatgg caaggcaact tcaaagacgt tttgagtgac cccgaggcca ataaattaat
      661 gtcagatttc gtggccgata aaatacggac tcgtgtaaat gaccctgaaa tagcagaaaa
      721 attaatccca aaagatcacg gctttggaac tagacgagta ccactggaga ctgaatattt
      781 tgaatcctat aacagggaaa atgtgaaact cattgatatc acagagaacc ccatcttgcg
      841 aatcacacca acaggaatat tgactgaaag agaagaatta gaattcgatt tcataatata
      901 cgccactgga tttaatgcta tcttgggtag ctatgacagg attggcattc aaggtacaca
      961 agaaaaaaaa ttaagagacc aatggaaaca agaattatct agtttccttg gactgcaagt
     1021 taacaatttt ccaaatctat tcatgatcct tggtcctcac gctctactgg gcaataatcc
     1081 tcgaagtatc gaatataacg ttgagtggat aacagatctc atccaacaca tgggagaaaa
     1141 aggttttaca cgagcggaag caacagctga ggccgttgcc agttggtatg agtatgttct
     1201 tgaacagggt gaaggcctgt tatctaatga agtggattct tggatgaccg gtgttaacag
     1261 caacctcgaa gggaggcaaa aaaggatcat tgccagatac agtggtagcc aggaatctta
     1321 ccgtagcagg tgtgacgaag tggcagcaaa cgggtatgta gaattgaatt tactctgatt
     1381 tgatggagct tcatacgtta atcatcggga aattttacag tttatatagt tccttctggc
     1441 caagatcaaa cacctctgat actgttccaa aaattcagat tacggtgagt ttcaatgcaa
     1501 ataaatttaa ccaaaaagaa gcttaaaagt ggggctccag catttggggc aattatccat
     1561 gaatattccc caggtaccgt ggaacttttc ggtgcgattg gatatgactt tgttatgatc
     1621 gactgtgaac atggcggcat gtctgtagac caagtagaaa atatggtcag aggggctgaa
     1681 gtgttcggaa ttacaccaat agcacgaatc ccgaatcatg atgcttccac tattttgagg
     1741 tttttggaca gaggagtcca aggaatcatt gttccccata tcaatacaag atctcaagca
     1801 gaaggtgtca cgaaggcagc tcggtatcat ccggaaggag ataggggggt cggtggaggt
     1861 agagcccatg actacggaat aaacactaca agatcagaat caaccaaatt cgtgaatgac
     1921 aatattctcg tgattccaat gattgaagag gtcgaagcta ttaagaatgt gagtgaaatc
     1981 gcttcagttg aaggagttga tgttttacat gttgctccgg gagacctttc tcaaagtatg
     2041 agtaatccgc ctcaatccga agtacgagaa actatggata aggtagtacc aaaaatccga
     2101 ggagcaggga aatgggttgg agtaggcggt aactctccca acgatgttga aagggtgtct
     2161 gacttgatca atttgggggc aaatatgata accattgcgg ctcaaggcct tatgaagagt
     2221 ggcgcggaat cattccgaaa atcagtagaa aaccatttaa cttaactgcc gtcaaaaatt
     2281 aacttcatat aggaggctat gtggtgcgat atttgattcg aaactctact gttgttacag
     2341 gaaacataaa ccgattaatt ctctataact catcaatatt tatccagaac ggtgttattc
     2401 aagatattgg ggaaagtgat ttacttgaag caaaataccc ttctgtgaca attatcgaag
     2461 gggtaggcaa ggtgattttc ccgggcctaa ttaattgcca cacacatctt ttggcgacag
     2521 ctgataaagg aattttggaa gattttggct ttcccacaac tttaaaattc ccaaccactg
     2581 gtcgtgggtt gctaaacgaa gatgagagga atgtgtttgc aagactcgct tgtatagaag
     2641 cgatcaagag cggaaccact actttgcttg agatatcaaa tgacattgca ggatatgccc
     2701 aaacattgga ttcggcaggt ttacgtgttt tcctcggtga aacgttaagt gatatcgatg
     2761 actccaaatc aaccgaaggg gtgtacgaat attcatctca taagcgtgac atagggattg
     2821 gaaaagcatc agatttgatc aatgcctggg atggcaagtc aaacgatagg ataagaggtt
     2881 ttgttgctcc gcacgctcca gagagagttt cgcctgacct actaagggat tcaagagatt
     2941 tggcggagaa gcatgatctt ggatatacca ttcatttgtc tcaaagctac aaggaaattg
     3001 aagccacaaa acgcataaga ggagttttac caactcatta cctttttgca aacgaatttt
     3061 tgagcaaccg gttagttgcc gcccactgta ggtacctgga cagttctgaa attgcaatgc
     3121 taggtcaaaa caatgcgggt atatctcata attcagctat aggtgctcgg aggggagcag
     3181 cagcacctgt acatgaacta ttatcatcgg gatgttcgat tggtatgggg tccgacaata
     3241 tggccgaaga tatggtggag gtgatgcgag ccgccctctt tcatgaaagg gttcggagaa
     3301 atgacgagat gaatcctcaa cccgaagata ttctcgagtg gggaacgatg ggtggagcga
     3361 gaacactagg tatcgtcgac gaggtaggat caattgagat aggcaaaaag gcagatcttt
     3421 ttatgataga tttccgcaaa gctcatcttg taccaactct caggatcgtt tcatctttta
     3481 ttcataatgg actgtcttcc gatgtcacag atgtgatgat agatggcgaa tgggttatgc
     3541 agaattcctc tattaaaacc atcaatgaac ctgaggttat tgaacaagcg gaagagatag
     3601 gacatagagt ttggaatcgt ttggttagag aaaaccctaa tgtgccgttc cctattagtt
     3661 tacctcctgg gccactttag gccagagtaa aatcacctaa atatatctat tcctttggca
     3721 gccagatttg gcaaatgtat acgggtactc tgctgattaa aggaatcacc ttcgatatcc
     3781 aggtagcgga ggttaggcaa ggaattgatc ggactaatat ctcttatgtt gttaaatgaa
     3841 agtccaagcc agttaatagt ggttatctta gaaagggcat ctatgttggt gacgcggttg
     3901 tgtcctacat ccaatgattc taaagctttc aatgtcgtca aaggaccgag gtcttgaatg
     3961 tagtttctcg cagcatggat ccaagtgact ccattaatga acgagagtgg gtcaagattg
     4021 gaaatttcat tactctcgat atccaaactt ttaagctcat ttaaggaaga gatactattt
     4081 agttgctcaa tattattttc gttgagattt aattccgata ggttacccat cccttcaagt
     4141 ggcgacaaat cgtctaggtc acaattcttt aggcgaagag aaaacagttc tcttaaataa
     4201 gacagatggg agaaatctgc tatctgggaa tcgctaacat ccaaatgcct taaatcggtt
     4261 aatttttcca atggagacac gttttccaaa ttcgcatggg aaatcctaag gctgtccaaa
     4321 agagctaatt cttgcaaggg agctaagcta acgtcccctt tgcaaaccag cgatagcttg
     4381 ataacactag gagtctttac taaatcgtcg atgtcaccct gtgtcgcctc ttcaatttgc
     4441 agggtcgtta tttttgacat ttgaattgga tctagttgat cctcataaac accaagttta
     4501 gccctgaccg ctttatcgaa attattgctc atacacccta acttgcaaat gaaagatata
     4561 ggttcatttg tagaattata ttccaatctc gatttcgcaa gtcgcctaat tcaaaaaatt
     4621 taaacctaag tcgccagata atttttgagt tttgtttttc aagatgatat aaatcgcctt
     4681 gctaaataat gcgaaatctt atcagtcggc taacaaaggt ggatcatggt aattgataac
     4741 tccattccag aaattaatgg accactaacg ggtataaaag ttttagattg gactatttgg
     4801 caatttggcc ctgttagcac ctcgatgatg ggagacatgg gtgcagacgt aataaaaatc
     4861 gagtctttgg atggtgatcc ttcccgcgga ctttcaaggg cttcaagtat gaagatggat
     4921 ttgggggaag gtcgcatcgc ctattttgag acatgtaatc gaaacaagcg aagtatcgca
     4981 gtgaacctaa aaactaaaga aggtcaacag attgtctacg acctttgcaa ggatgctgac
     5041 gtatttgttc aaaatttccg atctggcgta gctgaaagac taggtgtcgg ttatgaaaca
     5101 ttgaaggaaa taaaccctca attaatatat ggatcagcca atgggtatgg tccccatggc
     5161 ccagacgcgg gccaaccttc attcgatggc tgtgggcaag caaggtcagg cttgatgatg
     5221 tcagcaagcc aaggaggcca agaataccca acacaagtga ctcaaggtgt gtcagatcaa
     5281 attggaggaa tcatgctctg ccaaggcatt ttggcagctc tagtttcaag gagcataaga
     5341 ggaatagggc aaaaggttga gacatcacac ctgagcgcca atatgtggct gcagggccta
     5401 ggggtatcaa tgagtctttt acagggaggg gtagggtttt caccctataa tcgaaaagag
     5461 ccagtgaatc ccctttctaa tgtatacagg acgtcagatg gtcgattcat catgatgatg
     5521 catctacaac cagatcaata ttgggagccc ttattagaag caatggatct acaggaattt
     5581 atcggagatc cccgctattc cgatatgcca agtcggtcac aaaattcagc agaagtcgtt
     5641 gccttactgg acaaacgatt cgatgagaag acagcagaag aatgggatat cctttttaga
     5701 aatttctcta ccgatttcat ctactctttg gtccaaaccg tccaagattt accatatgac
     5761 cctcaggtga tagcaaacga ttttattaca aattttaatc acccagttct cggcgaagta
     5821 caaatgtgta atcatccttc tatatatagc gaaacccctg caggcatttg gaaggaagcc
     5881 ccggaagttg gccaacacac tgagcaagtt ttggtagacg aattaggata cagttgggac
     5941 gatatagcca aactacaaag cgcaggaaca attttataaa cacaaattta aaaggagaca
     6001 ccatgggaca gatagacggt ggtcaattat tcgcaaaagc cttaaaaaaa gaaggagttg
     6061 aatacgtttt caccctcaac ggtgggcata tttacaacct ttacgaaggg tgcgcagatg
     6121 aaggtattga gcttatcgat ttcagacacg aacaagttgc tgcacatgcc gcagaaggct
     6181 gggcaaaagt aacagggaaa ccaggtgttt gtatagtcac cgcaggtccg ggtgtcacag
     6241 atgctgtaac tggcatagcc aacgcattcc aagctcctag ccccatgata atggtaggag
     6301 gtaatgccgg gatcttcgac cacctaaggg gaggtctaca agattttgat agtgccacat
     6361 ttttgaagcc tgtggcgaaa ttttcagaac aggttaagag agtggagagg attcctgaat
     6421 atgtgtctaa tgccttccgt gctgcaacca gtgggatccc tggaccagca tacttggaga
     6481 tccccataga tatagttaac ggtgttaccg aagaagaaaa cgtcaaatat ccagacagat
     6541 accggaccga atcccgagcg tttggagacc cagaatatat tcaaaaggtt acagacatct
     6601 tgaagaatgc cgaacgaccg atggttcttg ctggatctga tatctggtgg tgtgatgctt
     6661 ccgcacaatt gagagagttt gtagagaaaa tcaacgcgcc ggtgttcttg aatgcgatgg
     6721 gtagaggaag tttacattcc gaccacccaa atctaggtag ccttgggcga aggtacggat
     6781 tagttaaatc tgatgccgta gttctgatag gaactcccat agacttcagg ctaagctatg
     6841 gtgaagatag actcttccct cagaatccaa agctaatcga aatcatgatg gatgcaaata
     6901 aaatcgggca aaacagggca gtcgatgttg gg
//
LOCUS       DASCNS010000159         9406 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001252535, whole genome shotgun sequence.
ACCESSION   DASCNS010000159 DASCNS010000000
VERSION     DASCNS010000159.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001252535"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="QF838_05800"
     CDS             complement(<1..709)
                     /locus_tag="QF838_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60228.1"
                     /translation="MNSLQEAEEKGQKRRKANFMWRFRRNKLAVVGLGIVVLCLFIAM
                     FANFLAPTSYDVGNLTEARQPPSWSHIFGTDAVGRDYLSRMLYATRTSIVVALGVQVI
                     TLTIGISLGTLGGYLGGWVDQIVVRIIEIATGIPALLIAMFIMGLLGHSMWNVIFALG
                     ISGWVVETRITRGQFLAFKEREFVIAAQAIGANRFRVAFRHIFPNIIPILAVLLAFQV
                     PAVIFNEAGLSFLGLGID"
     gene            complement(706..1638)
                     /locus_tag="QF838_05805"
     CDS             complement(706..1638)
                     /locus_tag="QF838_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60229.1"
                     /translation="MIRYSLGRLLGLLGSVLIISLILFIAVRWLPGTPWNEAEIPLQG
                     AAKENMMRKYGLDQPVHVQYAKYLWNLLHLDLGNSFIYKTETVWETIARGWPATAKIG
                     SVTLIIAFSIGIPVGIIAALRQNSLLDYTVTFLATTGITVPNFAVAVWLVMFVSVKMD
                     LLPTQGWGTWQHMVLPVVAYSLAPTSLIARFTRVSMLEAMRSDYIRTARAKGLAEPVV
                     ILRHVFKNALIPIITLVGPLIPNLMTGSIFIENTFGIPGIGRHFVTSIFDRDYPVIMG
                     VFMLVAVLFGLTYFISDILYTWADPRVRLTDRKT"
     gene            complement(1663..2733)
                     /locus_tag="QF838_05810"
     CDS             complement(1663..2733)
                     /locus_tag="QF838_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJO60230.1"
                     /translation="MVAEEAVRLARLIEHKGPYDRDVNDEVLTLFEDSRKGFGDYIGA
                     SSDEIALTRNVSDGINIVATGFDWLPGDEVIITNEEHPSGSLPFMNLSERYGVNVKLL
                     KFQTEPTQLLKDLESLVTDKTRLVFLSHVSTVDGARLPAREVGDFLKARDIPYMLDGA
                     HAVGQFPVSMKELGCDFYAGCGHKWLLCQQGTGFLYVKKDWITKLKTSWVGWGMTEEY
                     DLDSLSYTSLKTAQRFEFGTRYWATHASGIKALEFHNNIGPENIYRRSHDLASQLKRR
                     LIDIPGISIKTPMAKTDSTGIVAFSTAGLNHPEPGNWLWNEHRILTAHNPKTQSMRIA
                     AAFYLLEDEIDLLADKLSELAS"
     gene            complement(2926..4743)
                     /locus_tag="QF838_05815"
     CDS             complement(2926..4743)
                     /locus_tag="QF838_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HJO60231.1"
                     /translation="MRKNGFMFLVIGAMTALMLTVAIGCSSSEEDTAPAAAPAAAPAA
                     APAAAPAPATKAAPAAKTTNVFGREMPSDAAPMAAQFLKVNYEKEGESLEFPVSVYNS
                     AAGGIQSGFSLALLSTDLSTVPGGATSWSSSDDMKTWTFNIDPDLTWSDGVPLTAHDY
                     VYTWQYYADPEHAYDFTWYFGMLEVENYGAIEAGEKALDTLGVTATDDKTLVFQLDTP
                     APYVPGFMMYGSPLAKHAAEKHGQYYSNDPATTLSSTPWILKEWVPNQHAEFEPNVNY
                     TGKIKPFIENYKAVYGERKFDAYLAGEVDTINGPFTPADQEYMENDAGLMKERGLSPG
                     DFRTHYLFFDNQSAPFNDVRVRQAFAKAIDRDAIINNIVGSEAGEPRYGVLIPGFPDA
                     AGPDKLKQYQGLDVAAAQSLMADAGFAGGAGFPKLELALRQETELFQATAAAVAQQLK
                     ENLGVDVTINNMDRKTYMGHLNGHDLQFAMVSYGFDYVDASNFMGVFKTDGRHNWNNK
                     EFEDLRIEAGAMALSADRSAKMLKLQEILSEDVGSVFFWSQVQNQLHKPYLKGTWKNE
                     NEAGWSGLQFPNWNPGFGAQNIYEVYVGENVQEHTRSAY"
     gene            complement(4942..5937)
                     /locus_tag="QF838_05820"
     CDS             complement(4942..5937)
                     /locus_tag="QF838_05820"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO60232.1"
                     /translation="MLTTSAKGRAFDYNRVVGGRAFRGIVYATKGTGNDFFVVLRDTY
                     GSDVVKLTIGPDIDDEEIVISFREVLEENFNGSWPSCAVFNGTHLLVTDELRNIISIF
                     TPEGDLVKTMGGPGNGEIQFDRPSGITLDSDNNVYIADTLNHRIQKFSPDLEFTQSWG
                     KFGDAHGLLNAPWGICTDNDNFIYVSDHKNHRVQKFDSDGVFIMSIGEFGSSASNLDH
                     PSDVSVDQSGDIYVADWVNNRVQIYDTTGIHIAGLTGSAVELSKWQKQYVAASPDVHK
                     ARRRVSSLEPETLFALPTCVSFDDSNSRLLAVDSQRWRIQIFDKITNYSDPQFNI"
     gene            complement(5975..7117)
                     /locus_tag="QF838_05825"
     CDS             complement(5975..7117)
                     /locus_tag="QF838_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HJO60233.1"
                     /translation="MTEILSKDPVIVVVQLSGGNDYMNTVVPHSNSLYRDYRPSVNIN
                     ETDVIELTSEIGFHPAMAPLADMYKEGNVAIIHGVGYENSPRSHFRSMDIWHTCEPET
                     LGTEGWLGRAIKQIDPNKENVVTAVSMGPSLYRALVAPDVPVATVENLDNYGLLTDIS
                     PEQKRDRVLDRYKKMYSPMIGSGPVMEFLGQTGLDAMKGADILNVAPALYSSTVEYAD
                     SSIAKKLKGIAQIHLANLGTRIFYCDLGSFDTHADQLSTHNKLWSSASSAIRDFFDDL
                     EEHQAADNVTMFLFSEFGRRVYDNGAGTDHGAGGVCLAIGKNVKGGEYGQYPSMKEGD
                     LDQGDLVPDMDFRSVYTTLAEDCMKLDPVPIVGGHFEKPGFIGSSN"
     gene            complement(7171..8568)
                     /locus_tag="QF838_05830"
     CDS             complement(7171..8568)
                     /locus_tag="QF838_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO60234.1"
                     /translation="MVSTKANTEITVMAHLMRRAGFGASREELETRCEKGYTETVEEL
                     LAPLTQTPVDIYEFLRYYPMWWKPGTMGGLGQAPWVWTMINTASPLQEKLCIFYHNIF
                     ATGVAKVDHYDEIQDMIDMFREKGLGHYRDILLEVAKSPAMIYWLDNNENHADSINEN
                     WGRELLELFTMGVGNYTEEDVQECSRAFTGWTLEHKLPRFHMGRWDWHFKFIEEDHDY
                     GEKEFLGHKGNFDGEEVIDIILSSPATANFMARHIYNFFVEDEPQVPAWSVVPPNNPD
                     AVQFLADALMDSNYHMETVLRKLFNSDFFKESQFSRVKNPAEVVVSTLRLVGNSSLPG
                     PDILDWTGQIVYMGQDLLNPPSVEGWHSGVEWINSGTLMKRTNFMSEMVGDYSRPGIA
                     KIIARVSSTFFKPEDVVDACLDIMGPLKVIAETRDELIDHVSSQGDFDWQSTGVTRTL
                     ELLQLIVATREYQFA"
     gene            complement(8751..>9406)
                     /locus_tag="QF838_05835"
     CDS             complement(8751..>9406)
                     /locus_tag="QF838_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017050.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HJO60235.1"
                     /translation="IESLDPKSPRLQVGLRYTSQNSAHTDDMMMWMGSCAVSGDYRDI
                     LSSNKSHNQVQPQLAGIQITVSLYLGTSEGSVALKSATPDDKPIVNLNLLATESDVNR
                     MAEGVQMASGIMNGSYLRDIVDIRTSPTSNMLSDKTKFHEWLRSTTTTGNHLTSTCSM
                     GTSSNKFAVVDENCRVYGVDNLFIADASIMPDNVRANTNVTTMMIGERLSASLKNHG"
BASE COUNT         2547 a         2178 c         1963 g         2718 t
ORIGIN      
        1 catcaattcc aagtcctaaa aagctaagtc cggcctcatt aaaaatcaca gctggaactt
       61 ggaaggccag caggacagct aatatgggta tgatatttgg gaaaatatgt ctaaatgcta
      121 ccctgaatct atttgcaccg atcgcttgag ctgcgattac gaattctctc tctttaaaag
      181 ctaaaaattg accgcgagtg atacgtgttt ccaccaccca acctgaaatg cccaatgcaa
      241 atatcacgtt ccacatggaa tgcccaagta atcccataat aaacatcgct attagtaaag
      301 ctgggatacc ggttgctatt tctatgatcc taaccactat ctggtctacc catcccccaa
      361 gatatccgcc aagggtgcct aaactgattc ctatagttaa ggtgatgact tgcactccca
      421 aagctacaac gatagaggtt cgcgtggcat aaagcatacg actgaggtaa tcacgaccta
      481 ccgcgtcggt gccaaatatg tgagaccaac taggaggctg tcttgcttca gtcaaattcc
      541 ccacgtcgta tgaggtaggt gctaaaaagt ttgcaaacat agcgatgaat agacataaaa
      601 ctacgatccc caagccaacg actgccaatt tgttacgtct aaaacgccac atgaaattgg
      661 ctttgcggcg cttttggcct ttttcttctg cttcttgaag actgttcatg ttttgcgatc
      721 cgtcaatctc acccgagggt cagcccaagt atagagaata tcactgataa aataagtaag
      781 cccaaagagg acggctacca acatgaaaac acccatgatc actgggtaat cccgatcaaa
      841 aatactggtc acgaaatggc gaccgattcc aggtatcccg aaggtgtttt ctataaaaat
      901 gcttcctgtc atcaggtttg gtattaaagg ccccacgagg gtaataatag gaatcaaggc
      961 gttcttaaaa acgtgtcgaa gaattactac aggttctgct aatcccttgg cccgtgcagt
     1021 cctgatgtag tcggacctca ttgcctcaag catactgact cttgtaaaac gtgctatgag
     1081 gcttgtagga gcaagggaat aggccaccac tggcaggacc atatgttgcc aagttcccca
     1141 accttgggtt ggaaggaggt ccattttcac agaaacaaac ataactagcc acaccgcaac
     1201 agcaaaattt ggaacagtga tacctgttgt ggctagaaaa gttacagtgt agtccagtag
     1261 gctattttgt cgaagtgcgg caataatgcc cacagggatt ccgattgaaa aggctattat
     1321 aagtgtgaca gaacctatct ttgcggtggc aggccagcct cttgctattg tttcccaaac
     1381 ggtttcagtt ttgtatatga atgaattacc taaatctaag tgaagtaaat tccacaggta
     1441 ctttgcatat tgaacatgga ctggttggtc caatccatat tttctcatca tattttcttt
     1501 tgcggctccc tgcaggggta tttccgcttc attccacgga gtaccgggta accatctcac
     1561 tgcgataaac aggattaagg aaatgattaa aacggagcct aataaaccaa gtaatcttcc
     1621 taaagaatat ctaatcatgg tcagattttc tcttcatttg ggttaagatg caagttctga
     1681 taatttgtca gcaagtaaat ctatttcatc ctccagtaga taaaaagcag cagcaatccg
     1741 catcgattgt gttttgggat tatgggccgt caagattcta tgctcattcc atagccaatt
     1801 tcccggttca gggtggttaa gacccgctgt agagaaggcg actatccctg tgctatcagt
     1861 tttagccatc ggcgttttaa tagatatgcc tggaatgtcg attagacgtc tctttaattg
     1921 gctggccaaa tcatggcttc ttctatatat attttcagga cctatgttgt tgtggaactc
     1981 gagagcttta attcccgatg catgggttgc ccaataccta gtaccaaatt caaatcgctg
     2041 tgcagtctta aggcttgtgt aggataatga gtcaagatcg tattcctcag tcattcccca
     2101 gcctacccag cttgtcttca acttggttat ccagtctttc tttacataga gaaagccagt
     2161 tccttgttgg cataatagcc atttgtgacc acagccggcg taaaaatcac aaccaagttc
     2221 tttcatagag actggaaatt gacctactgc gtgagcccca tccaacatat aggggatgtc
     2281 tcgggccttc agaaagtctc caacttccct cgcgggtaac ctagcgccat ctacagttga
     2341 tacatggcta agaaaaacca gcctagtttt atcagttact agagactcca agtcttttaa
     2401 gagctgcgtt ggctctgttt ggaatttaag cagtttcacg ttgactccgt accgttcaga
     2461 taagttcata aacggaaggg aaccgctggg atgttcctca ttggtgatta tcacttcgtc
     2521 accaggaagc caatcaaatc cagtggccac tatattaatt ccatcagaca catttcgggt
     2581 gagtgctatc tcgtctgagg aagccccaat ataatcgcca aagccttttc tagagtcttc
     2641 aaataaagtc aacacttcat cattaacatc tcgatcgtaa ggccctttgt gttctataag
     2701 acgggccaac ctgacagctt cttctgccac cacgcttgga gtgggtccga aagttccaca
     2761 gtttaaatat gtgcaactct ggagtgcggg tatttgggtg cggattgatt gaatatccgt
     2821 tttgtgcctc cctaaaaaaa cggcccacct ggtacaaaaa gtaccggcag gccgctcaat
     2881 ttatttctct tgtttcttgc taatagcgag aaatcttata tgcggttagt aggctgatcg
     2941 agtgtgctct tgaacattct cgccgacata tacctcatag atgttttggg ctccaaaacc
     3001 aggattccag ttagggaact gaaggcctga ccaaccagcc tcgttttcat tcttccatgt
     3061 ccctttcagg tatggtttgt gcaactggtt ctgaacctgt gaccagaaga atacgctacc
     3121 aacatcctca gagagaattt cctgtagctt gagcatcttg gcagatctgt cagccgaaag
     3181 tgccatcgca cctgcctcta ttcttaagtc ctcaaattct ttgttgttcc agttatgtcg
     3241 accatccgtc ttgaagacac ccataaagtt tgaagcgtca acatagtcga aaccgtagga
     3301 aaccatagca aattgcaggt cgtggccatt tagatgaccc atgtaagttt tgcggtccat
     3361 attgttgata gtgacatcaa cgccgaggtt ttctttaagc tgctgggcaa ctgctgctgc
     3421 tgttgcttgg aagagctcgg tttcttgtct tagagctaac tccagttttg gaaaacctgc
     3481 gccgcctgca aagcctgcgt cggccatcag cgattgggct gcggcgacat caagaccttg
     3541 gtattgtttg agtttgtcag gtcctgcagc atctgggaaa ccagggatta atacaccgta
     3601 cctaggctcg ccggcctctg accctactat gttattaatg atcgcgtctc tgtctatagc
     3661 cttggcaaag gcctgtctga ctctgacatc gttaaacgga gcactctggt tgtcgaagaa
     3721 aaggtagtgt gttcggaagt cgccagggga tagacctctt tctttcatca accccgcatc
     3781 attctccata tattcctggt ctgctggagt aaacggtccg ttgatagtat cgacctcgcc
     3841 agcaaggtaa gcatcaaatt ttcgctcacc atacacggct ttgtaatttt ctatgaaagg
     3901 cttgatctta ccggtgtaat tcacgttagg ttcaaactct gcgtgctggt ttggtaccca
     3961 ttcttttagg atccaaggag tacttgaaag tgttgtcgcg ggatcatttg aatagtactg
     4021 gccatgtttc tcagcggcat gttttgccag gggagaacca tacatcataa acccgggaac
     4081 atatggcgct ggcgtatcaa gttggaagac aagggttttg tcatctgttg ctgtaacgcc
     4141 tagggtatct aaagccttct cgccagcttc aattgcccca tagttttcta cttcaagcat
     4201 accgaagtac cacgtgaagt cgtaagcatg ttctgggtca gcataatact gccatgtata
     4261 tacgtagtca tgagcagtta gtgggacacc gtcactccat gtaagatccg ggtctatgtt
     4321 gaatgtccag gtcttcatat cgtctgaaga tgaccaacta gtggcaccac cagggacggt
     4381 agaaaggtct gtgctcaata gggctaagct aaaaccactc tggattccac ctgcggcaga
     4441 gttgtaaaca ctgaccggga attctagaga ctcaccctct ttttcatagt tgaccttaag
     4501 gaattgagct gccattggag cagcatcact tggcatttca cgtccaaata cattggttgt
     4561 ttttgctgct ggggctgctt ttgtagccgg ggctggagcc gctgctggag ctgctgccgg
     4621 agctgctgct ggagctgctg ctggagcggt atcttcttct gaagaactgc atccgattgc
     4681 gaccgtaagc atcagggccg tcatggcacc aatcaccaaa aacatgaaac cattcttgcg
     4741 cattgagtaa cctccacaca atacttcttt aacaagccaa cgaaaacact cgtggctttt
     4801 cactcggtac attatattat gaaagtcaac aaaagataat actaaactac tgcaacatca
     4861 atattgcctt atgaaatggg ctgcatgtct aaccaaaata tcatgctcga gtatttgtcg
     4921 aaaccactga tgtcttagta atcagatgtt aaattgcgga tcgctataat ttgtaatctt
     4981 atcgaatatt tgaattcgcc atctttgtga atccacggct agtaacctag aattcgagtc
     5041 gtcaaagcta acgcatgttg gcaatgcaaa aagagtttca ggttctaaac ttgatacacg
     5101 tctccgagcc ttatgtacgt cggggctagc ggccacgtat tgtttttgcc attttgatag
     5161 ttcaactgct gaacctgtta agcctgctat gtggataccc gttgtatcgt aaatctgcac
     5221 cctgttgttg acccaatctg ccacgtaaat atcaccggat tgatctacag aaacgtctga
     5281 gggatggtcc aagtttgagg cgctggaccc aaactctcca atggacataa tgaaaacccc
     5341 atctgaatca aacttttgga ccctgtgatt tttatgatca gatacataga taaaattgtc
     5401 attatctgtg caaatccccc aaggagcatt taataatcca tgggcatccc cgaatttccc
     5461 ccaggattga gtgaattcta aatcggggga aaatttttgg atacgatggt taagagtatc
     5521 tgctatgtaa acattattat cggaatctaa cgttattcct gacggacgat caaattggat
     5581 ttcgccattc ccggggccac ccatcgtttt aacaaggtct ccttcagggg tgaagataga
     5641 aataatattt cgtagttcat ccgtgaccag tagatgggtg ccattaaaaa ctgcgcaact
     5701 aggccaagag ccattgaagt tttcttcaag gacttctcga aatgaaatta caatctcttc
     5761 gtcgtctata tcaggaccaa ttgtcagctt gaccacatct gatccatagg tgtctcttaa
     5821 aactacaaag aaatcatttc ccgtaccttt agtggcgtaa acgattcctc tgaacgcccg
     5881 acccccaaca acccgattgt aatcaaaagc acgtcccttt gccgatgtag ttagcatttt
     5941 aaatttacct ataatctgac taatatggct aagtttagtt tgaagaacct ataaatcccg
     6001 gcttttcaaa atgtccacca acgataggca cagggtctaa tttcatacaa tcttcagcca
     6061 gagtggtgta cacgcttcga aaatccatgt caggtactaa atcgccctga tcaagatctc
     6121 cctccttcat tgagggatat tgtccgtact ctccaccctt cacgtttttt ccaattgcaa
     6181 ggcaaacacc accggcccca tgatcagttc ctgcgccgtt gtcatacact cttctgccaa
     6241 actcagaaaa caaaaacata gtcacattgt cagcagcctg atgttcctcc aaatcatcaa
     6301 agaaatctct tatggcagat gaagctgagg accaaagttt attgtgggtg gaaagttgat
     6361 ctgcatgagt gtcaaaactt ccaaggtcac aataaaaaat ccgagtacct aaatttgcca
     6421 agtgtatctg agcgattccc tttagttttt tggctattga ggaatctgca tattcaacag
     6481 ttgatgaata caaggcaggt gcgacattta atatgtctgc tcctttcata gcgtcaagtc
     6541 ctgtttggcc gaggaattcc ataactggac cagatccaat cattggagaa tacatttttt
     6601 tgtatctatc taatacacgg tctctttttt gttcaggaga gatgtccgta aggagtccat
     6661 aattatctaa attttccact gtggctactg gaacgtcagg agctaccaaa gcccgataga
     6721 gtgacgggcc catacttacg gctgtgacca cgttttcttt attggggtcg atttgcttga
     6781 tggcccttcc aagccaacct tccgtgccga gagtttcagg ttcacatgtg tgccatatgt
     6841 ccatggatct aaaatgagac cggggagaat tctcatagcc aaccccatga ataatggcaa
     6901 cattaccctc tttatacata tccgccaaag gggccatggc aggatgaaaa cctatttctg
     6961 aggtgagctc aattacgtca gtctcattta tgttaactga aggtctgtag tctctgtata
     7021 aagaattact gtggggcacg acagtattca tataatcatt gccaccagat aattgaacta
     7081 caactattac ggggtctttt gataggattt cagtcatatt tatcctcgtt attcgctatc
     7141 cttttcgtta caactttttc acaaggccgg ttaagcaaat tgatattctc tggtcgcgac
     7201 tataagttgg agcaattcta aggttcttgt caccccggtt gattgccagt cgaaatctcc
     7261 ctgtgaagaa acgtgatcta taagctcatc ccttgtctca gctataacct ttaggggtcc
     7321 catgatgtcg agacaagcgt ctactacgtc ttcaggttta aaaaacgtgg agctaacacg
     7381 agctattatt tttgcaatac ctggtcgcga atagtcaccg accatttccg acatgaagtt
     7441 ggtacgcttc attaaggtgc cactgttgat ccactctact ccagaatgcc acccttcgac
     7501 actgggagga tttaagaggt cctgacccat atagacgatc tggccagtcc aatccagtat
     7561 atctgggccg ggaagactac tatttccaac caatcttaga gtacttacca caacttcagc
     7621 tggattcttg accctggaga attgagactc cttaaaaaaa tcactattga ataattttct
     7681 caagacagtt tccatatggt agttactgtc cattaaagca tcagccaaaa attggaccgc
     7741 atcggggttg tttggaggaa caacagacca tgctggtact tggggttcgt cttctacaaa
     7801 aaagttataa atatgcctag ccatgaagtt agccgttgca gggctagaaa ggatgatatc
     7861 tattacctcc tccccatcaa agtttccttt gtgtcccaaa aattcctttt ctccataatc
     7921 atgatcttct tctatgaact tgaagtgcca atcccatcta cccatatgga atcttggtaa
     7981 cttgtgttct agggtccatc cagtgaaagc ccgggagcat tcctggacat cctcttccgt
     8041 ataattcccc acacccattg taaatagttc caacaattcc cgaccccaat tttcgttaat
     8101 agagtccgca tgattttcat tgttgtccaa ccagtaaatc atagctgggc ttttggctac
     8161 ttcaaggaga atgtccctat aatggccaag gcctttttct cggaacatat cgatcatgtc
     8221 ttggatttcg tcgtagtgat caactttagc aactccagta gcaaagatat tgtgatagaa
     8281 aatgcataac ttttcctgga gcggtgaggc agtattaatc attgtccaaa cccatggggc
     8341 ttggcctaat ccgcccatgg ttcctggttt ccaccacatt gggtagtatc ttaaaaattc
     8401 atatatatca acaggagttt gagttaaagg agccaacaac tcctctacag tttctgtgta
     8461 tcccttttcg cacctggttt cgagttcctc tcgggaagct ccaaatccgg cccttctcat
     8521 aaggtgtgcc atcacggtaa tttctgtatt ggcttttgta ctaaccatgc accctactcc
     8581 aaatttgtct tgaatttttg accttggcaa atgagataca gaatagcaga aaatttccaa
     8641 attgcacgat ttgcccctaa ggaaattatc ggggctgata tcgaaataaa ctatcaggga
     8701 tatcggcact agcgacggta ggcggtttta ttcctaaatg tgaatcatcc ttaaccgtga
     8761 tttttcaaag aagcggacaa cctttcccct atcatcattg ttgttacatt ggtgtttgct
     8821 ctgacattat ctggcattat ggaggcatca gcaatgaata gattgtcgac cccgtacact
     8881 cgacaatttt cgtctacgac cgcaaattta ttagaagagg ttcccatgga acaagtgctt
     8941 gttagatggt ttcctgtagt tgttgtggag cggagccatt cgtgaaattt ggttttatca
     9001 ctgagcatgt tgctcgtcgg actcgtccga atatctacta tgtccctcaa gtaagaacca
     9061 ttcataattc cagaggccat ttggacgcct tcagccatcc tattaacatc actttcagtt
     9121 gctagcaaat ttaaattcac aataggtttg tcatctgggg tcgcagactt aagggcaaca
     9181 cttccttcac tggtcccgag gtaaagagaa acggtgatct gtatccctgc tagctgtggt
     9241 tgaacttggt tgtgagattt gttcgaagac aatatgtctc tataatctcc gcttactgca
     9301 caagaaccca tccacatcat catatcatct gtgtgggccg aattttgaga cgtgtatctt
     9361 aaaccaacct gtagccgtgg agatttggga tccaaagatt ctatat
//
LOCUS       DASCNS010000160         6836 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001258008, whole genome shotgun sequence.
ACCESSION   DASCNS010000160 DASCNS010000000
VERSION     DASCNS010000160.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001258008"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="QF838_05840"
     CDS             complement(<1..1175)
                     /locus_tag="QF838_05840"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60236.1"
                     /translation="MPKNLMRVKEEEQINVQEKPLSKNALEGVRVIDLSWIVAGPQCT
                     RLLADFGAEVIKVENEAALDYCRNILPIDADGSPNRSGLFNTLNRNKKSVTLNVMHPT
                     GMEKLKELISISDVIVENFSSRVLEKWGLSYEDQKKIRPDIIYCSMSGFGHSGRDRDY
                     VTWGPTAQALSGLTFMSGLPGEESAGWGFSYMDHTGGFYGALAILMALKHRNSTGKGQ
                     HLDLSQVEAGIGMTGTSILDYTVNSRPFRREGMPPGNTAPDKKIAPHNSYRCRGKDRW
                     CVITVTNEEEWYALSKAINNPLWINDKKFSTMALRYENQDELDQHIETWTINRTPHEV
                     MHHLQSFGVPAGAIQTPRERVEEDPQLKERDFLPTIKHGELGDTKVEAMPMKMSETP"
     gene            1295..2233
                     /locus_tag="QF838_05845"
     CDS             1295..2233
                     /locus_tag="QF838_05845"
                     /inference="COORDINATES: protein motif:HMM:NF021287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HJO60237.1"
                     /translation="MRVGEFELVDPVPEMNNTVAIGMLRPWVDVGRVGTLSLRKLEQY
                     LGAKELGRLAKPGKFFDFTRYRPRMRITNGKRVFTKPNTIVHHAKDQYSGRDYIFIHI
                     REPHNFGEEYTDSIVDLFNHCNVTEYCRVGGMYDSVPHTRPILVTGSMTDEQEGKAGS
                     LLNPRRSTYQGPTSIINQVNEDLTERGVQNTTLMAHLPQYVQLDEDHLGASRLLEVLC
                     AVYGFPADLAEKSKGDQQYKDIERAIDYGGEVGTLIKQLETYFDRVLSRSEGQEDSEN
                     LSEEDVEINLSSDVQDFLNEMGERFEDEGSDSSQRN"
     gene            complement(2230..3045)
                     /locus_tag="QF838_05850"
     CDS             complement(2230..3045)
                     /locus_tag="QF838_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO60238.1"
                     /translation="MLFRKIREMEFPAVGMGTSRTFDIDDSIPEVFSVRNKIVEECQN
                     NGVTLVDSSPMYGNAEKVLGLAIQENDPSKFQIATKVWTTGYRSGLDQIDTSFSLLKT
                     EYIHILQIHNLVDWKTHLPTLTSLKNEGKIGLVGLTCMTPSDYPEMMNIMKTGKIDSI
                     QIPYNVINTRVESDVLPLAESMEIGVLVMEPLQKGKYVKDLRISPDLGPLSDLGIKTW
                     AQALLSWVISNEVITSAIPATSNPKRIKENSLGAIQMPKELRDFVKHETERCI"
     gene            complement(3055..3402)
                     /locus_tag="QF838_05855"
     CDS             complement(3055..3402)
                     /locus_tag="QF838_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60239.1"
                     /translation="MNGKYYFSAAMDVPQDKEALFNEVYDKEHIPYLSEVPGVLAIAR
                     FTTEPLKMVIGGVEQEIIIENQPKHTAIYEIEDPKVLTSPQWAAAVDKGRWATEVRQH
                     TFNRRHVLLKNKN"
     gene            complement(3451..4371)
                     /locus_tag="QF838_05860"
     CDS             complement(3451..4371)
                     /locus_tag="QF838_05860"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HJO60240.1"
                     /translation="MKIAIIGQAAFGKDVLNALVDKKENVVAVLCPPDNPGRPEDPIK
                     SAALENQIPVFQYKRMRDKEAIREFLSLHIDLCVMAFVTDIVPDEILEGPRLGTIQYH
                     PSLLPEHRGPSSINWPIIQGKSKTGLTIFWPDKGLDTGPILLQKEFDITETDTLGSIY
                     FGKLYPAGVDAMVEAVEMVKNGTAPKVEQDHSKATYEGWCRSDDVSIDWNKPGIEIYN
                     LVRGSDPSPGANTVWNKKSIYLFNTSFESSKHDQPPGTVTKISDESFSLSVSDGFINI
                     GRVKVDKEAKIMASDFVESHGLKVGDRFGL"
     gene            4520..5767
                     /locus_tag="QF838_05865"
     CDS             4520..5767
                     /locus_tag="QF838_05865"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60241.1"
                     /translation="MAEVKKSMSPLSGLRVLGVTVYLAGPFAMMNLARLGAECIKVEM
                     PGVGDPSRGNGPFASPDGYVETQESEKHLSTRFLKRAQGLKSVTLNLKDPKGLEMFME
                     LVKKSDVLVENLSPGAMKRLGLSYEKVAKVNPGIIYTSISGYGQSGPYSHQKAHDPQI
                     QGMSGLMDINGSESEPPTRVGFYIGDLVTPLFACYSVLAALREKDKTGYGQYLDVSMM
                     DSLTSLMFMENLEEAIMYGEPLRQGNNSRGGPMGLYHTKDGDITLTVASDDQWSRLAK
                     ALDAPELTVNPKFATFIERNWNVVEAREEVQGLLDRYTRNEAIALLEENKVPCGLVRT
                     VPEIIEDSHFWDRGTLQPMKSSAFADAVPGIASGFPVDFSGGELPQSKGAPMLGEHNH
                     EIYHEVLGISSEDLEGYERNGII"
     gene            5792..6694
                     /locus_tag="QF838_05870"
     CDS             5792..6694
                     /locus_tag="QF838_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HJO60242.1"
                     /translation="MRGQAKPLRTMLYVPANKEDWVRKAPKYGADALILDIEDSVPLD
                     EKQNAREIVSRMVPELAAQGITVLVRVNDIDTGLTAEDVAYAVQEGLYAITLPMVKGP
                     ADVKKLDSFISSEEKKKGIDLGKILIDPGLETATGLRFAYDVGTSSDRVAHMGAGGGR
                     GGDIARAVGYRWTPEGTETLFIRSKVLLDARAAGVPFPLTGLWQDIHDHEGLRSFAQH
                     SRELGYNGMTVIHPSHVPIVNEIFSPSKDEISEWQDLVQSMKEIRESGGAAVSFQGGM
                     VDIAHEKTALEMLDMARALGLLDD"
BASE COUNT         1858 a         1320 c         1524 g         2134 t
ORIGIN      
        1 cagggtgttt ctgacatttt cattggcatg gcttctactt tggtatctcc tagttctcca
       61 tgtttaatag tgggtagaaa atctctttct ttaagttgag ggtcttcttc tactctttct
      121 ctaggagttt gaattgcacc agcaggaaca ccaaaacttt gaagatgatg catcacttcg
      181 tgtggggttc tgttaattgt ccaagtttcg atgtgctgat ctagttcatc ttggttttcg
      241 taccttaatg ccatcgtaga aaattttttg tcatttatcc acaaagggtt gttaattgct
      301 tttgataagg cataccattc ctcctcattg gttactgtaa ttacgcacca acggtctttc
      361 cctcgacacc tgtatgaatt atggggagca atttttttgt ctggggctgt gtttcccggt
      421 ggcatgcctt ctcttctgaa cggtctgctg tttacagtgt aatcaaggat agaagtgccg
      481 gtcattccta taccggcctc gacttgagat aaatctaaat gctgcccctt gccagttgaa
      541 tttctatgtt tcagagccat caatattgct aatgctccgt agaatcctcc tgtgtgatcc
      601 atatatgaaa aaccccaacc ggccgactcc tccccaggta gccccgacat aaatgttaga
      661 cctgaaagag cctgtgctgt tggaccccaa gttacataat ccctatctct tccactgtgt
      721 ccgaatcccg acatgctgca ataaatgata tctgggcgta tctttttctg atcctcgtag
      781 ctgagtcccc atttttccag tactctagaa ctaaaatttt ctacgatcac atcagaaata
      841 gaaatgagtt ctttcagttt ttccatacca gtcgggtgca tcacatttag ggtcacactc
      901 tttttgttgc ggttgagagt gttaaacaaa ccgcttctgt ttggtgaacc gtcagcatcg
      961 attggtagga tatttctgca gtaatcaaga gcagcttcat tttctacctt aatgacttct
     1021 gctccaaaat ctgcgagtaa tcttgtgcat tgaggcccag ctacaatcca agataaatct
     1081 atgaccctta ccccttctaa tgcattttta ctgagtggtt tttcttgcac gttaatctgt
     1141 tcttcctctt ttactcgcat aagatttttg ggcattatac agggataatt tcctgttttg
     1201 agcctgatat gaataatacg gctggtgtat aattctgagc ctatagagtg attttatcta
     1261 agtaaatttt tcattttggt ggttgaacag gtttatgaga gttggtgaat ttgaactagt
     1321 tgatcctgtg cctgagatga ataataccgt ggccatcggt atgttacgtc cttgggtaga
     1381 tgtgggaaga gttggtacct tatcgttaag gaaattagaa caatatcttg gtgctaaaga
     1441 acttggccgg ttggccaaac ctggaaaatt ttttgatttc accagatatc ggccaaggat
     1501 gcgtatcaca aacggcaaac gagttttcac taaaccaaat accattgtcc atcacgccaa
     1561 agatcagtat tcgggtcgag attacatttt catccacata agggaacctc ataattttgg
     1621 agaggaatat actgactcaa tagttgattt gttcaatcat tgtaatgtga ctgaatactg
     1681 ccgagttggt ggtatgtatg actcagtccc acatactagg ccaattttag ttacaggctc
     1741 catgacagac gagcaagaag ggaaggccgg atctctattg aacccccgtc gtagtaccta
     1801 tcagggacct acgtccataa ttaatcaggt taatgaagat ttgaccgagc gaggcgtcca
     1861 aaatactact ttgatggcac atttgccaca atatgtccaa ttggatgaag accatttggg
     1921 tgcatctagg ctgttggagg ttttgtgtgc tgtgtatggg tttcccgcgg atctagcaga
     1981 gaaaagcaaa ggagaccaac agtacaaaga tattgaacga gcgatagact atgggggaga
     2041 ggtgggcact ttgataaaac aattggaaac ctactttgat agagttctta gtcgatctga
     2101 aggccaagaa gattcggaaa atttaagtga agaggatgtg gaaattaacc tatcgtcgga
     2161 tgttcaagat ttcttaaatg agatgggtga gcggtttgag gatgagggaa gtgatagtag
     2221 ccagaggaac taaatacacc tttcagtctc atgtttgaca aaatctctca attcttttgg
     2281 catttgtatt gcacctaaag aattctcttt tatccttttg ggattagatg ttgctggtat
     2341 tgctgatgtt ataacctcat ttgatataac ccaagacaac aaggcctgag cccaggtttt
     2401 gatgcctaaa tcagataaag ggcccaagtc aggacttatt cgaaggtctt tcacgtattt
     2461 tcctttttgt agtggttcca ttaccagaac tcctatttcc attgattccg ctaacgggag
     2521 tacatcagat tccactcgtg tgttaataac attgtaggga atttggatag aatcgatttt
     2581 gccggttttc ataatgttca tcatttctgg atagtcacta ggagtcatac aggttaggcc
     2641 cactaaacct atctttcctt catttttcag acttgtaaga gttggtaggt gggtcttcca
     2701 atctactaaa ttatgtattt ggagaatatg aatgtactca gttttcagca acgaaaatga
     2761 ggtatcaatt tggtctaaac cagatcgata gcctgtcgtc cacacttttg tagctatttg
     2821 aaattttgag ggatcatttt cttgaattgc taatcctagg actttttctg cgttgccata
     2881 cattggtgag gaatcgacga gggtcacccc attattttga cattcctcca ctattttatt
     2941 tcgaacggag aaaacttctg gaatcgaatc atctatatca aaagtcctgg aggttcccat
     3001 accaacggca gggaattcca tttctcttat ttttctgaac aacatttgct acacttaatt
     3061 tttatttttt agtaatacgt gccttctgtt gaaggtgtgt tgtctgactt cagtagccca
     3121 tctaccttta tccacagcag cagcccattg tggggatgtc aggacctttg gatcctctat
     3181 ttcatagatt gctgtgtgct tcggttggtt ttctataatt atttcttgtt ctactcctcc
     3241 gattaccatt ttcaatggct ccgtggtaaa tctggctatg gctaatactc caggcacctc
     3301 agacagatat ggtatgtgtt ctttgtcata tacttcgtta aatagagctt ctttgtcctg
     3361 aggtacatcc attgcagctg agaaatagta ttttccgttc atttgctaac tcctttcggt
     3421 agataaaaat taataaaggt taataaatgt ctaaagtccg aacctgtcgc ctactttcag
     3481 tccgtgggat tcaacaaaat ctgaggccat aatttttgct tctttgtcta ctttgaccct
     3541 gcctatattg atgaagccgt ccgagaccga caaactgaac gactcatccg atattttcgt
     3601 tacggttcca ggaggttgat catgtttgga agattcaaaa gatgtgttga ataagtaaat
     3661 tgactttttg ttccacaccg tattggctcc agggctagga tcagaccctc taaccagatt
     3721 atagatttct attccaggtt tattccaatc tatgcttacg tcatctgacc tgcaccaccc
     3781 ctcgtatgtg gctttgctgt ggtcttgttc aactttgggg gcggtaccat ttttaaccat
     3841 ttcgactgct tcaaccattg catccacccc agcagggtag agtttgccaa aataaattga
     3901 tcccaaagtg tcggtttcag taatatcaaa ttctttttgt aaaagtattg gacctgtgtc
     3961 gagacctttg tctggccaga aaatagtgag tccagttttt gatttccctt gtatgatggg
     4021 ccaattaatt gaactaggcc ctctatgttc tggaagtaaa gatggatgat actgaatagt
     4081 tcccaagcga ggtccttcaa ggatttcgtc tgggactata tcggtaacaa aggccatcac
     4141 acatagatct atgtgtaacg atagaaattc tctaattgct tctttatctc gcattctttt
     4201 gtattggaat actggtattt gattttctaa tgctgccgat tttatgggat cttcaggtct
     4261 acctggattg tccggtggac aaagcacagc tactacgttt tcttttttat ctaccagtgc
     4321 atttaagaca tctttgccaa aggcagcttg tcctattatg gcgattttca tatcaatatc
     4381 tcctatacgg tcatttggga ggtatggtcc acgctggaat ataacacatc aattgaccgt
     4441 acctctgggc gaacataaca tgagagcagg taattacaga ttcaattact tataagacca
     4501 ttttagagaa gaggttaatt tggccgaagt taaaaaatcg atgtctcctt tgtcgggcct
     4561 tagggtactg ggtgtgactg tctacctcgc aggaccattt gcgatgatga atttagctag
     4621 attgggggcc gaatgtataa aagtagaaat gccgggtgtg ggagatccat ctagaggaaa
     4681 tggacccttt gcctctcctg acggatatgt ggaaacgcag gagtctgaaa agcatctttc
     4741 tactcggttt cttaaaaggg ctcagggtct gaaaagtgtt actttgaatc taaaagatcc
     4801 aaaaggccta gaaatgttta tggagctagt gaaaaaatca gatgtgctag tcgaaaattt
     4861 atcgcccggc gctatgaaga gattaggttt gagttacgag aaagtagcaa aggttaatcc
     4921 gggaattatt tatacttcta tttcaggata tggtcaatcc ggtccgtatt cacaccaaaa
     4981 ggctcatgat ccacaaattc aaggcatgag tggccttatg gatataaacg gatcagaaag
     5041 tgaacctcct accagggttg ggttttatat tggagatttg gtaacacctc tttttgcctg
     5101 ctattcagtt cttgcagcgc tcagagaaaa agataagact ggttatggtc aatatttgga
     5161 tgtgtccatg atggattcat taacctccct catgtttatg gaaaatttgg aagaggccat
     5221 tatgtacggt gaaccactta ggcagggaaa taatagccga ggaggcccga tgggactcta
     5281 tcacaccaag gatggagaca tcaccttaac tgttgccagt gatgatcagt ggagcagatt
     5341 ggctaaggcg ttagatgctc cggaattaac agtaaatccc aaatttgcca cctttatcga
     5401 acgaaattgg aatgttgtcg aggctcgaga ggaagtacag ggtctacttg atcgttacac
     5461 tagaaatgag gcgatagcat tattagagga aaataaagtt ccttgtgggt tggtaagaac
     5521 tgtgccggaa ataatagaag actcacattt ttgggatcga ggaacccttc agccaatgaa
     5581 aagttcagct tttgctgatg ccgttccagg aattgcatca ggttttcctg ttgatttttc
     5641 tggtggtgaa ttaccccagt cgaaaggtgc accaatgtta ggtgagcaca accatgagat
     5701 atatcatgag gttctcggga tcagctcgga agatcttgaa ggatatgaac gaaatggaat
     5761 catttaaaat taataaaaaa gggaggcatt gatgaggggc caagctaaac ctttgcgtac
     5821 catgctttat gtacctgcta ataaagaaga ttgggtcagg aaagctccaa aatatggtgc
     5881 agatgctctt atcttggata ttgaagactc tgtaccttta gacgagaagc aaaacgcacg
     5941 ggaaatcgtg tctagaatgg ttccagaatt agctgcgcaa ggtataacag tattggtaag
     6001 agtcaatgat attgatacag gacttactgc agaagatgta gcttacgcgg ttcaggaagg
     6061 cctttacgct attactctgc cgatggtgaa aggacctgca gatgtaaaaa aacttgactc
     6121 ctttatttct agtgaggaga agaaaaaagg tatagatctg gggaagatat tgatcgaccc
     6181 tggcttggaa acagctacag ggctcaggtt tgcttatgac gtgggaactt catcagatcg
     6241 agttgcgcat atgggggctg gaggtggtag gggaggagac atagctagag ctgtaggata
     6301 tcgatggact cctgaaggca cagaaacttt atttatccgc tcaaaagtac tattagacgc
     6361 tcgagcagct ggtgtacctt ttccattgac tggcttgtgg caggacatac atgatcatga
     6421 aggattaagg agcttcgcgc agcattcgag agagctaggc tataacggaa tgacagttat
     6481 acatccgtct cacgttccaa tagtgaatga aatattttct ccgagtaaag atgagataag
     6541 tgagtggcaa gatttagttc aatcaatgaa ggagattaga gagagtgggg gggccgccgt
     6601 ttcttttcaa ggcggaatgg ttgatattgc tcatgagaaa acagctttgg agatgcttga
     6661 tatggctcgt gctttgggat tgttggatga ttaaccagac gtattgataa tttcattcac
     6721 tgattgatca atcatttcct ttaaattttg acctggattt ccatcttcat tccaatgctc
     6781 tccccaagag taaatcgact tgttggcgac gacggctcct aatgtcctga ccattg
//
LOCUS       DASCNS010000161         5904 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001259616, whole genome shotgun sequence.
ACCESSION   DASCNS010000161 DASCNS010000000
VERSION     DASCNS010000161.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001259616"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="QF838_05875"
     CDS             complement(<1..333)
                     /locus_tag="QF838_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HJO60243.1"
                     /translation="MSIRFIQMADPQFGMFSSISKLTEEEAISRSREGLTLRYVEQEF
                     DGFAPETQLFSKAIRYANEYKPDFVVMCGDMVHEADSEDQRNELFRITSQLNKDIPIY
                     WVAGNHDVG"
     gene            complement(353..1516)
                     /locus_tag="QF838_05880"
     CDS             complement(353..1516)
                     /locus_tag="QF838_05880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60244.1"
                     /translation="MWPKNEKQNQLLEKSQKIAKYISPTSLKHDRDGSFPHEHFRFMR
                     EIGYLAAAVPESYGGPGYGLTDILLAQFEIGTGDGSTALAVGMHHMVVGTEAIARKWP
                     DSIRRRVFSEVVSNGALINNIASEPELGSPQGGGRPSTTLTPNGEGEWDLEGRKTWST
                     LAPELTYAVTFAAVEDGTGDTARVLQSMDAKGVYVEDTWDSMSMRSTGSHDIIYKAVK
                     VRDEDFLFRSNYKSTSSDPIGGSAWFPLMTSAANLGVANAARNYAINFAMNRKPTGAP
                     DPISKIPHVREQIGRMESKLIISKRALFSFSEDWENYPEFQPELFREIGAIKIKSIDS
                     AIEITDLAMKVVGGVGLEKSRPLERYFRDVRSGLFNPPIEGRALEQLASAVLD"
     gene            complement(1519..2370)
                     /locus_tag="QF838_05885"
     CDS             complement(1519..2370)
                     /locus_tag="QF838_05885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HJO60245.1"
                     /translation="MKIGISMSATASMGDFGVVATKIEDLGFDSVWLPEHPVMPVNHK
                     SVYGGTPDGSIPKQMYLGVNPLIALSRASSVTTNLGIGTAVNLITEHNPLDLAKLIAT
                     LDFYSGGRFLFGIGTGWFREEAEIMGADFDHRWTQAREYVLAMKEIWTKEEAEFHGKY
                     IDFPPIICNPKPAQNPHPPVLLGGNAKNVLKRVAKWGDGWLPIRLSPEDIVKAQQELL
                     KLCDTEDRDPSTIQITMHSLPPDKDLLSRFEEIGVDRVLVGVASATQEEIFKELEVIA
                     ESILVNG"
     gene            2498..3136
                     /locus_tag="QF838_05890"
     CDS             2498..3136
                     /locus_tag="QF838_05890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO60246.1"
                     /translation="MPEQNQNRVQWVYSSRNNSELTIRYDEWSKDYDKDLIEDFGWTA
                     PKTAADFLAKYIRPDSLVLDAGAGTGLVGMALAAQGFQNLTGMDMSRGMLDQADQKGV
                     YARLDQMTLGESLEYDSSVFDAVISVGVMTLGHATPASFDELVRITKPNGYIVFTIRT
                     DVYLNNGFKEKQEEMAANKLWEVIEVSDEFRPLPVGEPDVMHQVWVYQVLDY"
     gene            complement(3148..4041)
                     /locus_tag="QF838_05895"
     CDS             complement(3148..4041)
                     /locus_tag="QF838_05895"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011619112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HJO60247.1"
                     /translation="MSLVVRPDYHAIPTIEKNGLDWISTHESQTQGRNALRIGILNIM
                     PLGEQYEMNILNPLALSLVDIEPVWIKLESHSYKTWPEGHVDDLYATYSGAVKEKKLD
                     GLIVTGAPVEHLDFEEVNYWDEISELIEDARNNSHSTLGLCWAGFALAKLSGLGKITF
                     ERKLFGVYELDNLTYNHPIMGALDDKFVCPQSRFAGFEDQEVEDAVSRGDVNALAHGK
                     ESGYTILETPDHKQLMHIGHPEYNKGRLAYEARRDQGNPKIPNIENFDFDNPLNRWRM
                     HRNTFFQRWINFVTQESRSLR"
     gene            complement(4108..5433)
                     /locus_tag="QF838_05900"
     CDS             complement(4108..5433)
                     /locus_tag="QF838_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011132276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HJO60248.1"
                     /translation="MSEQRFETLQLHAGQSPDPATNARAVPIYQTTSFVFDNAEHGAK
                     LFALEEFGNIYTRIMNPTTDVFEKRVAALEGGVVGLATASGQASQFAAIANMMQAGDH
                     LVSTSFLYGGTYNQFKVQFPRLGINVSFTDGDEPEKFEALIKENTKAIYVESMGNPKF
                     NIPDFEALAELAHSHGIPLVVDNTLGAAGALIRPIDHGADVVVQSATKWIGGHGTSIA
                     GIVVDAGTFDWGNGKFPLYTEPSEAYHGLVHWDAFGFGSDICNMLGVPENRNVAFGLR
                     SRIEVLRDYGASISPFNSFLLLQGLETLSLRVERHGSNALDLAKWLESNPKVESVNYP
                     GLDSSPYKAAAERYLERGYGSMLTVSLKGGYEDAVKFIDSLELASHLANVGDAKTLVI
                     HPASTTHQQLSEEEQLSSGVEPTMVRVSVGLEHIDDIKEDFQQALDSIN"
BASE COUNT         1662 a         1383 c         1126 g         1733 t
ORIGIN      
        1 gccaacatca tggttaccag caacccagta gattgggatg tctttattta attgagaggt
       61 tatcctgaac agttcgtttc tctggtcctc tgagtccgct tcatgaacca tgtccccaca
      121 cataactaca aaatcaggct tatattcatt cgcgtaccga atagccttgc taaataattg
      181 tgtttcaggt gcaaacccat caaattcttg ttctacataa cgcagagtca atccttccct
      241 tgatcttgaa attgcttcct cttccgtcaa tttactaata gaagagaaca ttccaaattg
      301 aggatcagcc atctggataa agcggataga catgtaatta ctccaaaaag ggttaatcta
      361 atactgccga agctagttgt tccaaagccc taccttcaat aggaggatta aacaacccgc
      421 tcctgacatc tctaaaatat cgctctaagg ggcgagattt ttctaatcct actccgccga
      481 ctaccttcat cgctagatct gtaatttcta tcgccgagtc tatggatttt atcttaatgg
      541 cacctatttc acgaaaaagt tcaggttgga attcaggata attctcccag tcttcagaaa
      601 aggaaaatag agctctttta gatatgatga gtttggactc catacgaccg atttgttccc
      661 tgacgtgagg tatttttgat atcggatccg gtgcccctgt tggctttcgg ttcatagcaa
      721 agtttattgc ataatttcga gccgcatttg caacccctaa attcgcagca ctcgtcatga
      781 gaggaaacca ggctgaaccc ccaatcgggt cactgctagt gcttttataa ttggatctga
      841 ataaaaaatc ctcatccctt acctttacag ctttgtaaat aatgtcatgc gatccagttg
      901 aacgcattga catggaatcc caggtatctt ctacataaac gcctttagca tccatgcttt
      961 gtaggacccg tgcggtatca cccgtcccgt cttccacagc cgcaaaagtt acggcatatg
     1021 ttaactctgg agccagtgtg gaccaggttt tacgaccttc caaatcccac tcaccttccc
     1081 cattcggagt tagagtggta gaaggtcttc ctcctccttg gggtgacccc agctctggtt
     1141 cagaggcaat attgttaatg agggcgccat ttgaaaccac ttcactaaaa actcttctcc
     1201 ttattgaatc cggccatttt ctcgcaatcg cctcggttcc aaccaccata tgatgcatac
     1261 ctactgctaa ggcagtagag ccatccccag tcccaatttc aaattgggca aggagaatat
     1321 cagtcagccc atagcctgga ccaccatagc tctccggaac ggctgcagct aggtatccta
     1381 tttcgcgcat aaatcggaaa tgctcatgcg gaaatgaacc atcacggtca tgtttcaaag
     1441 aagtcggaga tatgtacttt gctatttttt gggacttttc caaaagctgg ttttgctttt
     1501 cattcttagg ccacatagtt aaccgttgac caagatagat tcggcaataa cttccagttc
     1561 cttgaaaatt tcttcttgtg tagctgaagc gacccccaca agtactctat cgacccctat
     1621 ttcttcaaat ctcgagagca aatccttatc aggaggaagg ctatgcatcg ttatttgaat
     1681 ggtagaagga tctctgtctt cggtatcaca aagttttaaa agctcttgtt gagccttaac
     1741 aatatcttca ggagaaagtc taataggaag ccatccgtct ccccactttg ctacccgctt
     1801 taaaacattc ttggcgttcc ctcccaacaa tactggaggg tgaggattct gagccggttt
     1861 aggattacaa attataggag ggaaatctat atatttgcca tggaattcag cctcttcttt
     1921 ggtccaaatt tccttcatcg ctaagacata ttctctagcc tgggtccatc tgtgatcaaa
     1981 atcagctccc ataatttcgg cctcctctct aaaccatcct gtacctattc caaataagaa
     2041 acggccgcct gaataaaaat ccaatgtagc tattagtttg gccaagtcca aaggattatg
     2101 ttctgtaatt aaattaacag ctgttcctat ccctaaattt gtggtgaccg atgaagctct
     2161 agaaagagca atcaaaggat taacccctag atacatttgc ttcgggattg acccatcagg
     2221 agttcctccg tagacagatt tatgatttac aggcatcacc ggatgctctg gtaaccaaac
     2281 tgaatcgaaa cctaaatctt caatcttagt ggctactacc ccaaaatctc ccatcgaggc
     2341 cgtcgcactc atactaatac caattttcat tgttcctcct atcaaacgtg actcatgtta
     2401 atacatcact ctgcatagta aaagaaatga tgaagacaca ttctgttaac ctaatcggtg
     2461 atctactaaa cgacatccga aatattgggg gttataaatg ccagaacaaa atcaaaaccg
     2521 tgtccaatgg gtctattcct cgaggaataa ctcagaatta actataaggt acgacgaatg
     2581 gtccaaagat tacgataaag atttaatcga agatttcggt tggactgcac ccaaaacagc
     2641 agccgatttc ctagctaaat atattcggcc tgattcgctt gtacttgatg ccggagcagg
     2701 aactggtctg gtaggtatgg cattagctgc ccaaggattc caaaatttaa cagggatgga
     2761 tatgtcgaga ggtatgctag atcaggccga ccaaaagggt gtttacgcac ggctcgatca
     2821 aatgacgctt ggggaatctt tggaatatga ttcctctgta ttcgatgctg tgattagtgt
     2881 gggagtaatg accttagggc acgccacccc tgcatccttt gacgaactag tcagaatcac
     2941 caaacccaat ggctatattg tattcacaat acggactgat gtgtatttga ataatggctt
     3001 caaagaaaaa caagaggaga tggcagcaaa taaattgtgg gaagttatag aagttagtga
     3061 tgaattccga ccattgcccg tcggggaacc tgatgtcatg catcaagttt gggtatacca
     3121 agtcttagat tattagcacc ggtccattta tctcagactc ctagattctt gagtgacaaa
     3181 attaatccat ctttgaaaaa aagtgtttcg atgcatccgc cacctattta atgggttgtc
     3241 aaaatcaaaa ttttcaatat tgggtatctt aggattcccc tggtcgcgcc tagcttcata
     3301 ggcaaggcgt cctttgttat attcagggtg cccaatgtgc attaactgtt tatgatcagg
     3361 ggtctcaagg atcgtgtagc ccgattcttt tccatgggcc aaagcattga cgtcccccct
     3421 actgacagca tcttccacct cttgatcttc aaaaccagca aacctgctct gggggcacac
     3481 gaatttatca tccaaggctc ccataattgg atgattgtag gtcaaattat ctaattcata
     3541 aactccgaat aattttcttt caaaggttat ttttccaaga cctgaaagct tggcaagtgc
     3601 aaagccggcc caacacaagc ccaatgtgct gtgggaatta ttccttgcat cctcaattag
     3661 ttcagatatt tcatcccaat aattgacttc ttcaaaatcc aggtgctcaa ctggggctcc
     3721 agtcactatt agtccatcta attttttttc tttgacggcc cccgaataag tagcatacaa
     3781 atcatccaca tggccttctg gccatgtttt ataggagtga gattccagct ttatccagac
     3841 aggttcgata tctacaagag acaaagcgag tgggttcaat atgttcattt catattgctc
     3901 accaagaggc atgatgttta atattccgat acgcagggca ttacggccct gggtttggga
     3961 ttcgtgtgtt gaaatccagt caaggccatt tttttctatt gtaggtattg cgtgatagtc
     4021 gggtcttact acaagagaca ttgctcacct tgaggttaat ttagggtcgc atgaccttgt
     4081 tccgagatca tacgacccta taatcctcta gtttatagaa tccaaagcct gctggaaatc
     4141 ctctttgatg tcatcgatat gctcgagccc tacagaaacc ctgaccatcg taggctcgac
     4201 tcctgaagat aattgttctt cttcagacaa ctgctggtga gttgttgagg caggatggat
     4261 taccaaagtc ttggcatctc ctacattggc gaggtgactt gcaagttcaa gactatcaat
     4321 gaatttaact gcatcttcgt agccaccttt gaggcttacg gtcaacattg acccatatcc
     4381 tctctccaaa tatctctccg cagccgcttt atatggactg gaatccaatc ctgggtaatt
     4441 tactgattca actttcgggt tagattccaa ccatttagca agatcaagag cattcgaacc
     4501 atgcctttcc accctcaaag acaatgtctc taacccttga agcaatagga atgaattaaa
     4561 aggactaatt gaggcgccat aatctctaag aacttctatc ctagacctta gtccaaatgc
     4621 aacatttcga ttctctggta cacctagcat attacaaatg tcgctgccaa aaccaaaggc
     4681 atcccagtga accaaaccat gataggcttc gcttggttct gtataaagag gaaatttgcc
     4741 attaccccaa tcgaaggtgc cagcgtccac tacgatacca gcaatactgg tcccgtggcc
     4801 tcctatccat tttgtcgcac tctgaacaac cacatcagct ccatgatcaa tcggcctaat
     4861 aagagcccca gccgcaccta aagtattatc taccaccaag ggtattccat gggaatgtgc
     4921 taattcagct aaggcttcaa aatcaggaat attgaattta ggatttccca tagattcaac
     4981 ataaatagcc ttcgtatttt ctttaattag ggcttcaaat ttctcgggtt catcaccgtc
     5041 cgtaaagctt acgtttatac ccaatcttgg aaactgtact ttgaactgat tgtaagtccc
     5101 accataaaga aaagatgtcg ataccaagtg gtctcctgct tgcatcatat ttgctatggc
     5161 tgcaaattga cttgcctgac ctgaggcagt tgccaaaccg acgacgccgc cttcaagtgc
     5221 ggccaccctt ttttcaaaca catcagtggt agggttcatg atacgggtat atatatttcc
     5281 gaactcttcc aatgcaaata acttcgctcc atgttcagcg ttgtcaaaga cgaatgatgt
     5341 ggtttggtaa ataggcactg ctcttgcgtt ggttgcagga tctgggcttt gaccggcatg
     5401 taattgcaaa gtttcaaatc tctgttcact catagtatct ccttagtttc tggaatttca
     5461 tctgatttag gtatcttaag catccgaatt ttcaataaaa tcataggcaa aaaaaaaccc
     5521 cacctaatga ctcaggaggg gtttttcttc atcgattccc cgctgaatca attcatacag
     5581 caacccatat cacaacacat catatgcgcg aaaacaatgt tgttgttctg acttaattct
     5641 tgtggtttca tagcgcgaaa actttacctt tcctttgcta tcatgtcaaa ttgaagaaga
     5701 atcaaaagat aaattaccca ttgatcaata aatcattttt catttgagac tacttagcag
     5761 ctttgaatat cttgagtttt ccaaatttgg ctttaattcg agatgttacc cccttctcca
     5821 aaacatttgt tttactggtg tcaccaccaa aaactcccct tatttcagga ctgtcagaat
     5881 tagggtgtac aatgaaactt gcac
//
LOCUS       DASCNS010000162         3725 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001264052, whole genome shotgun sequence.
ACCESSION   DASCNS010000162 DASCNS010000000
VERSION     DASCNS010000162.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001264052"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(96..995)
                     /locus_tag="QF838_05905"
     CDS             complement(96..995)
                     /locus_tag="QF838_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012512.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HJO60249.1"
                     /translation="MSIPTLTDVIAAQKRIQNYITRTPLHRHLALEKIVGTEMYVKHE
                     NHQKLGAFKVRGGVNLLAQMEPQDLKKGVSTASSGNHGQSIAYAANIFGARAFIAVPE
                     IANPGKVESMRNLGAEVIHYGEHFGIADQYMRDLSKEKGYKYINAVEETQLYAGVGTY
                     TLEIIEDLPEVDIIIVPVGGGSGACGTSITAKSINPKIEVIGVQSERAPAAYNSWKSG
                     DLEQAEMSSIAEGLATNKGYEPAQTILRKMLDDFILVSDEEMEDAIILYLEHTRNLVE
                     HAGAASLAAAIKIKERLKNKKWP"
     gene            1147..2373
                     /gene="lhgO"
                     /locus_tag="QF838_05910"
     CDS             1147..2373
                     /gene="lhgO"
                     /locus_tag="QF838_05910"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HJO60250.1"
                     /translation="MGTIDKQYDVVVVGGGIIGLATSMKLTQDFPNLKVAVLEKEKEV
                     AQHQTGHNSGVIHAGIYYAPGSQKANFCSTGGKLLRDFCNEYGIPYDMCGKLIVATDD
                     SEIPQLEELFKRGTENGAEGLRMVDQEEIKEIEPYSAGVKAILSPNTGIIDYFEVSQA
                     YATRMRENGGDLLTNVEVISIENKDGLVYLETTSGTVVAKYVLNCAGLHADTVARMMG
                     VDVGVKIVPFRGEYFSIIPEKEHMVKGLIYPVPNPSMPFLGVHFTRRINGSVEAGPNA
                     VFAFAREGYKKTDVNLKDTLGTLSYSGFWKMSAKYWKVGMHEQYRSLVRGVFVKSLQK
                     LMPEITGDDLGDPGAGVRAQVIDSNGGLLQDFAIEASANAIHVLSAPSPGATSSLTIS
                     EYIVDLAQESFDLSAA"
     gene            2395..3342
                     /locus_tag="QF838_05915"
     CDS             2395..3342
                     /locus_tag="QF838_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HJO60251.1"
                     /translation="MKIAYAFRRTTVYPYTAGFYGPAGWGLPDEPGLTTFLKKINEIG
                     FDGIELGFEAFGGYEATESSAKELQKRLADSGAPCVAIRAGGVLSTPVVGEQNRDRLF
                     KSIEIAGWMGIEIVNSALSGPSRNKTLGDNRPGKPVQQGSSQLASYQDFERTASILHE
                     AGERAGGGGLEVTVEVHQHSIADTSTSTLKLLEMANSDHVFANPDLGNILWHYDEPEE
                     SSEDAILALAPHSKYWHCKTLQRVHVPELDRAYFLRVPLPDGDIDYRFAIDAMIDAGY
                     EGYFALEGANTGDHISKDARSVQYVRKIVAEIEQGKPIL"
     gene            3464..>3725
                     /locus_tag="QF838_05920"
     rRNA            3464..>3725
                     /locus_tag="QF838_05920"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1006 a          731 c          895 g         1093 t
ORIGIN      
        1 taattgctgg cttgatagga ttcaccataa ctaatctttg tacctttcga tggcacggag
       61 caaatgctcc attgatagat ttccaccact tgctattatg gccacttttt atttttcaat
      121 ctttctttta ttttaatggc tgctgctaga cttgcagctc ccgcgtgctc cactaaattt
      181 cgtgtatgtt ccaggtacag aatgatggca tcttccattt cttcatcaga aacaagaata
      241 aaatcatcca gcatctttct caaaattgtt tgtgcaggtt cgtagccctt attggttgcc
      301 aaaccttccg ctatgctgct catttcagcc tgttctaagt cacctgattt ccaagaattg
      361 taggctgctg gcgcccgttc ggattgaacg cctataactt ctatttttgg gttaatggat
      421 ttggctgtaa tagaggtgcc acaagcgcca cttcctcctc ccacaggcac aattattata
      481 tcaacctcag gcagatcttc tattatttca agtgtgtagg ttcccacacc tgcgtaaagt
      541 tgggtttctt ccacagcatt tatatatttg tagccttttt ctttcgatag atctctcatg
      601 tattgatctg ctatgccaaa atgctcgccg taatgaatca cttctgcacc caaattgcgc
      661 atcgattcca ctttaccggg attagctatt tcagggacag ctataaatgc tctcgctcca
      721 aatatatttg cagcataagc tatggactgg ccatggttgc cacttgaggc tgttgaaaca
      781 ccttttttta agtcttgagg ttccatttgg gcgagaagat taaccccacc tctgaccttg
      841 aaagctccca atttttggtg attttcatgt tttacataca tttctgtccc aactattttt
      901 tctaacgcta gatgtcgatg aagaggagtt ctagtaatgt aattttgtat ccttttttgg
      961 gcggctatca cgtcggtaag cgttggtata gacaaaaaaa actcctgagg aaaggtttcc
     1021 ataatttata tgtcaagaaa tattttacgc ttaatatcgg tgacaaacac cactgtgctc
     1081 atgacatgct aaaatcctcg gcacttggtc ttctttaaat ttaagacact ttaaataggt
     1141 gagtgaatgg gtacgatcga taaacaatat gatgtggttg ttgtcggcgg gggcatcatc
     1201 gggcttgcca cttcgatgaa gctgacacaa gacttcccaa atttaaaggt tgcggttttg
     1261 gaaaaagaaa aagaagtggc tcaacatcaa acaggtcaca acagtggtgt aatccacgca
     1321 gggatttatt acgcgccggg tagtcagaag gcaaatttct gctctaccgg tggaaagctt
     1381 ctccgagatt tttgtaacga atatggaata ccttatgaca tgtgcgggaa actgatcgtt
     1441 gctacagacg attcggagat tccacaactt gaggaacttt ttaagcgtgg gacggaaaat
     1501 ggcgctgaag ggttgagaat ggttgatcag gaggaaataa aagagatcga gccatattct
     1561 gcaggagtta aagcaatatt atctccaaac acaggcataa tagattattt tgaagtttct
     1621 caggcgtacg ctactcgtat gcgggagaat ggaggggatc ttctgacgaa tgttgaggtg
     1681 atttctatcg aaaataaaga tggtctggtt tacctggaga ctacatcagg cacggttgtt
     1741 gcaaaatatg tcttaaattg tgccggtctt catgccgata ctgtagccag aatgatggga
     1801 gttgacgtgg gagtgaaaat tgtcccattc agaggggaat acttttcaat tattcctgag
     1861 aaagaacata tggttaaagg tttgatttat ccggttccaa accccagtat gccatttcta
     1921 ggagttcatt ttactcggag aataaatgga agtgttgagg caggtccgaa tgcagtcttt
     1981 gcatttgcga gagaaggtta caaaaaaaca gatgtgaatt taaaagatac cttggggact
     2041 ctctcgtatt caggtttctg gaagatgtct gccaaatatt ggaaagttgg tatgcatgaa
     2101 caatataggt ctcttgttag aggagtcttt gtgaaatctt tgcaaaaatt aatgcctgaa
     2161 attaccggtg atgatttggg agatcctggg gctggggtaa gagctcaagt gatagattca
     2221 aacggtgggt tgttacaaga ttttgcgatt gaggcctcag cgaatgctat acatgtactt
     2281 agtgctccgt cacctggggc tacctcttcg ctgactataa gtgaatatat tgtggatttg
     2341 gctcaagaat cttttgacct gagtgccgcc taatttttta caaggagtgt tgctatgaaa
     2401 attgcctatg ccttcaggcg gacaacggta tatccataca cagcagggtt ttacgggcct
     2461 gccgggtggg gacttccaga tgaacctgga ttaacaacat ttctaaagaa aattaatgaa
     2521 atagggtttg atggtattga gttgggattt gaggcttttg ggggttatga ggccacagag
     2581 tcttcagcta aggaattaca gaaacgcctg gcggactcag gcgccccgtg tgttgcaata
     2641 agggctggtg gcgttctttc tacacctgtc gttggcgagc aaaatagaga tcgcctgttt
     2701 aagtctattg aaatagctgg gtggatgggc atagaaatcg tcaatagcgc tttgtcggga
     2761 ccatctcgaa ataagacatt aggagataat agaccaggca agccggtaca acagggttca
     2821 agccaacttg cttcatatca agatttcgag cgaacggcca gtatcttgca cgaagcaggc
     2881 gaaagggctg gtggaggtgg actagaagtt actgtagagg tgcatcaaca ctctatagct
     2941 gatactagta cttcaactct gaaattactt gaaatggcga atagtgatca tgtttttgct
     3001 aaccccgatt tgggtaacat actttggcac tatgatgaac ccgaagaatc cagcgaagat
     3061 gctatattag ccctcgctcc ccactccaag tattggcatt gtaaaactct gcagagagtc
     3121 catgtgccag agttggatcg tgcttacttt ttaagggtgc ctttacctga tggagacata
     3181 gattatagat ttgctatcga cgccatgata gacgctgggt acgaaggata ttttgctttg
     3241 gaaggggcaa acaccggcga tcatatctct aaagatgcaa gaagtgtcca atacgtcaga
     3301 aagatagtgg ctgaaataga gcaggggaaa ccaattttgt agtatttgtt ttacttgact
     3361 ccatcagtca gtgagattaa catgtacgtc tgttgcgttt tttagcgcac tttttgggtt
     3421 catcccgacg caccttaaca actggatagt agaaggaaac cgttatattt agagtttgat
     3481 cctggctcag gacgaacgct ggcggtgcgc ctaatgcatg caagtcgaac gagcgacccg
     3541 ggcttgcccg gttagctagt ggcggacggc tgagtaacac gtaagtaatc tgccccgaag
     3601 agggggataa tccagagaaa tctggcctaa taccccgtac cttcctctcc agcctgctgg
     3661 attggaagaa aggtctaggc cgctttggga ggagcttgcg gcttatcagg tagttggtgg
     3721 ggtaa
//
LOCUS       DASCNS010000163         2308 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001270990, whole genome shotgun sequence.
ACCESSION   DASCNS010000163 DASCNS010000000
VERSION     DASCNS010000163.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001270990"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="QF838_05925"
     CDS             complement(<1..608)
                     /locus_tag="QF838_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HJO60252.1"
                     /translation="MTTRKFPKKAPLQEISIVRREDHTKDLALMWLEKPEGYTFKPGQ
                     YCTIGHDGIERAYSIASAPHEEFIELFIELVPVEEGGVLTPMLWDLDVGDKVTIRPRA
                     KGIFTFKEEMENQFLVSTVTGVVPYVSFIRDYLKQNRSGHHFYVLEGASYIDEFVYDK
                     EFENLSDINPSLVTFVPTISRPDEDRNSTWSGDTGRVNNIVK"
     gene            complement(642..>2308)
                     /locus_tag="QF838_05930"
     CDS             complement(642..>2308)
                     /locus_tag="QF838_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012602.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJO60253.1"
                     /translation="KLENHRNGDYALVASARGTNEDNFVAQKFARRVMKSNNVNVSSN
                     SRPKLVDSLQGLIGDDCATNKIWDLLDSQVILLVSANITEEQNVASVPIKQAVAKGAK
                     LIVIDQRETELTRYATKWIRPLTNSEPVLIGGILRSIIDQSLEDHDFATEKCEGYVEF
                     RNSLWDFDLVKVSAQTGISQEEIREMSQIFANTEASSILYGLETISDTHIDDTVKSLV
                     NLSLITGNLDKKSGGIYPLFSGANQQGSRDVGCVPNLLPGHRPTASLGIDLTEISEAC
                     DSGTIKALHLIGDELFHNTASRSDFLDKIKATDLLIVHESFANEVTNIADVVLPSTLF
                     TEKNGTYTNMEGRIHRLRPATGPKGDEDEDWRILSQIAARLGSGNLSYSSSDDVLTEI
                     TNEVETYKTLQIDNLDSRGHIRKPALNSSYLFSPIEFTQSEETLVNDQYPMVFAPGRV
                     LFDADRDAGIITENDLNTITRREIIEMNEEDAKKAGISEGDAIKILGEEFELTGNAHL
                     NGLHSGMVATTTLFGALIESLNNSSKPDPMSKIEGLKLCKVRIEKV"
BASE COUNT          570 a          507 c          464 g          767 t
ORIGIN      
        1 tctttcacga tattgtttac tcttcctgtg tcgccagacc aagtggaatt tctgtcttca
       61 tcaggccggc tgatggtggg aacaaaagtg accaaggaag gatttatatc acttaaattt
      121 tcgaactctt tgtcgtatac aaactcatct atataactgg ccccttctag gacgtaaaag
      181 tgatgtccgc ttcggttttg cttaaggtag tctcgtatga agctaacgta gggaaccaca
      241 ccagtaaccg tagaaactag aaactgattt tccatctctt ctttgaatgt aaatatacct
      301 ttagctcttg ggcgtatagt cactttgtca cctacgtcca gatcccaaag catcggggtc
      361 agtacgcccc cctcttccac agggactaac tctataaata attctatgaa ctcttcatgt
      421 ggcgcagaag ctatagaata tgctctctca attccatcat gcccgattgt gcagtattgg
      481 ccgggcttga aggtgtaccc ctctggtttt tcaagccaca tgagtgccaa atctttggta
      541 tgatcttctc tccgcactat gctaatttct tgtaatggtg cctttttggg gaattttctt
      601 gttgtcataa atactcctgt gatactctgc taccgttgtg gtcagacttt ttctatgcgt
      661 actttgcaga gtttcaagcc ctcaatttta gacattggat ctggtttgga actattattc
      721 aacgattcga ttaaagcgcc gaataacgtg gtagttgcaa ccattcctga atgtaggcca
      781 tttaagtgtg cattgccggt taattcaaat tcctcgccca atatcttgat tgcatcacct
      841 tcagagatac cagccttctt tgcatcttct tcattcattt cgataatttc acgcctggtg
      901 atggtattta ggtcattctc cgttataatt cccgcatctc ggtccgcatc aaaaagtact
      961 ctacctggtg caaaaaccat aggatattga tcattgacca aggtttcctc gctttgtgtg
     1021 aattcgatag gagagaaaag gtagcttgaa ttcaaggctg gttttctgat gtgtcctctg
     1081 gaatctagat tgtcaatctg aagggttttg taagtttcta cctcgtttgt tatttcagtt
     1141 aacacatcgt ctgaggagga ataagacaga ttacctgatc ctaatcttgc ggctatctga
     1201 gatagtattc tccagtcttc atcctcatcg cctttcggcc ctgttgcagg tctaagccgg
     1261 tgtattctgc cttccatatt agtgtaggta ccattttttt cagtaaaaag ggtcgatggc
     1321 aatacaacat ctgctatgtt ggttacttca ttggcgaaac tttcatggac tatcagtaaa
     1381 tctgtggctt tgattttgtc cagaaaatct gatcgacttg cagtgttatg aaacaactca
     1441 tccccgatga ggtgcaaagc ctttatagtt ccggagtcgc aggcctcgga tatttctgtt
     1501 agatctatac ctagtgaggc tgttggtctg tgcccaggca acaagttagg tacgcaccca
     1561 acatctctag acccttgctg gttggctcct gaaaatagag ggtatatgcc gcctgacttt
     1621 ttatccaaat ttccagttat tagagataaa ttgactagag atttaactgt gtcatctatg
     1681 tgggtgtcag aaattgtctc aagtccatac aggattgaag aagcctcagt attagcaaat
     1741 atttgagaca tttctcggat ttcttcttga cttattcctg tctgggcact tactttcact
     1801 agatcaaaat cccaaaggga gtttcggaat tcgacatagc cttcacattt ttccgtggca
     1861 aagtcatgat cttctaagga ttgatctatt atgcttcgca aaatcccacc tatcaaaaca
     1921 ggttctgaat ttgttagagg tcgaatccat tttgtggcat atcttgttaa ttcagtctcc
     1981 cgttggtcta ttactatcag tttggcccct ttggccactg cctgttttat ggggactgaa
     2041 gcaacgtttt gttcttctgt tatgttcgca gaaactagta gtattacctg tgaatccaat
     2101 aggtcccata ttttgtttgt ggcacaatca tcgcctatta aaccctgcag agaatctacc
     2161 aattttggcc gagagtttga ggagacgttt acattgttgg atttcatcac ccttcgggca
     2221 aatttctgag ccacaaaatt gtcctcattg gtgcctcttg ccgaagcgac caaggcataa
     2281 tcaccattcc tgtgattctc taatttgc
//
LOCUS       DASCNS010000164         2245 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001275746, whole genome shotgun sequence.
ACCESSION   DASCNS010000164 DASCNS010000000
VERSION     DASCNS010000164.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001275746"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="QF838_05935"
     CDS             <1..126
                     /locus_tag="QF838_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013026.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HJO60254.1"
                     /translation="RTRRTWSPNIHKATIEIDGQMKKVSLCTRCLRTQYKTAMKD"
     gene            282..1253
                     /locus_tag="QF838_05940"
     CDS             282..1253
                     /locus_tag="QF838_05940"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HJO60255.1"
                     /translation="MAEYSKPEAVDWAKENLRGQWSTLMTPFTSEDEIDEEGLRRDIR
                     HVKSLGTKGAGCTWGMGEFWTLTKEERFRVYDIVSDEAGNNWPIGAHVSHTSLKEMLD
                     LASYAEHAGFDLLIVAAPYFATRTEDQVIDWVGSLADNTNLAIMYYNSPQFGTVLDAN
                     GLERICNISNVVGVKEASFNPELSIDTHLKIGKQAIISTPDEWIFQKGKELGFEQQVM
                     FANTSDWRFDTPDSNYYVQFIDKVMEGDLDKDFYNTNIKPIKEVSDKWWKYTVQKMGG
                     ALPASLCKYWGELMGMSGGHVRAPLTDLTDDEKKRLKNDIAAATGRL"
     gene            1295..>2245
                     /locus_tag="QF838_05945"
     CDS             1295..>2245
                     /locus_tag="QF838_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016367.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HJO60256.1"
                     /translation="MILMVSVQDLYEAKQALKGGADIVDVKNLQEALVGSAHPNVVKE
                     IRDEVPSAHHASVTLGVVPNQIGTVSMAVYAAGILDATSVKVGFMVSEYDVALETLLA
                     AKEALIGTQTKLIGSLFADNLLYDGGIDPDLMVQLAKEGQCDGFLIDTLVKDGRNLFD
                     FVPEDRLKEMVLEGKELGMSTALSGHLKMSDLDELSRVNPDIVGVRGAVCQKGDRDSR
                     VHWESVSEFKKQLDLRQTGEIDVYPENRTSSVNQESNGWIIIDGTGKTCAGIIATLSE
                     QINADTASFVEVIIPDVLNTYDLIVWAEKNSHDIVTQRKDA"
BASE COUNT          738 a          428 c          516 g          563 t
ORIGIN      
        1 cgaacgcgaa ggacatggag tccgaacatt cacaaagcca ctattgaaat tgatggccag
       61 atgaaaaaag tcagtttatg cactcgctgc ttgaggaccc aatacaaaac ggcgatgaaa
      121 gactgagaac taagtaataa agctctgtag ttctatttat acgtcaatcc ttgcgttgac
      181 actgcttgcc ttgccttact attattaatt ctgttcattt ctagtattag ctgcttgtga
      241 agcagctata tgaataagtt cacatattga ggttcatttg catggctgaa tacagcaaac
      301 ccgaagcggt tgattgggcg aaagaaaatc ttcgcgggca atggtccacc ctaatgaccc
      361 cttttacgtc agaagatgag atagatgaag agggacttcg cagagatata cgacatgtaa
      421 aatctttagg taccaaaggt gctggttgca cctggggcat gggggaattc tggactctaa
      481 ctaaagagga aagattccga gtctatgaca ttgttagcga tgaagctgga aataactggc
      541 caattggagc tcatgtgtcc cacacatctt taaaagaaat gctagatctg gccagctatg
      601 ccgaacatgc tggatttgat ttactaattg tggctgctcc ttattttgct acacgaactg
      661 aagatcaagt gatagattgg gtaggaagct tagccgacaa cacaaatttg gcgattatgt
      721 attacaactc tccacaattt ggaactgttc tagatgcaaa cgggttagag cgcatttgca
      781 atatttccaa tgtagttgga gtcaaagagg ccagtttcaa tccagaactt tcaattgaca
      841 ctcacttaaa aataggtaag caagccatca taagtacccc cgatgaatgg atattccaaa
      901 aaggaaaaga actaggtttt gaacaacaag ttatgtttgc caacacctct gactggagat
      961 ttgatacacc tgactccaac tactatgtac agtttattga caaagtgatg gaaggggacc
     1021 ttgataagga tttctataac actaacataa agccaattaa agaggtttca gataaatggt
     1081 ggaaatacac tgtgcagaag atggggggag cattacctgc ctccttatgc aaatactggg
     1141 gtgaactcat gggcatgtca ggcggacatg tcagggcacc actgaccgat cttacagatg
     1201 atgaaaagaa acgattaaaa aatgatatag ccgctgccac aggcaggctg taaatagaaa
     1261 cacagaacag aattagtcac gaggaataca caaaatgatt cttatggtaa gtgtccaaga
     1321 tttatatgaa gcaaagcaag ctctaaaagg cggagctgat atagtcgatg tcaagaacct
     1381 tcaagaagcc ctagtaggtt cggctcatcc taacgtagtg aaagaaatca gagatgaagt
     1441 tccaagtgca caccacgcaa gtgtcacctt aggagttgtg cctaaccaaa taggcaccgt
     1501 ctctatggct gtttatgccg ccggaatcct ggacgcaact tcggtcaaag taggcttcat
     1561 ggtgagtgaa tacgatgtgg cactagaaac gttattagca gcaaaagaag ctctaatagg
     1621 aactcaaact aaactaattg gctcactttt tgcagacaat ctactctatg acggtggcat
     1681 tgacccagac ctcatggtgc aattggcgaa agaaggtcaa tgtgacggtt ttttaataga
     1741 tacacttgta aaagacggta gaaatttatt cgactttgta cctgaagatc gtctgaaaga
     1801 aatggttttg gagggaaaag aattgggcat gagcacagcc ctgtcaggcc atttgaagat
     1861 gagtgacctt gacgagctat caagagtcaa tcctgatatt gttggagtca gaggagccgt
     1921 ttgccaaaaa ggagatcgtg actctcgggt tcactgggaa tctgtatcgg agttcaaaaa
     1981 acaactagat ctcaggcaaa ccggcgaaat agatgtatat cctgaaaata gaacctcatc
     2041 cgtaaaccaa gaatcaaacg gatggataat aatagatggc acaggaaaga cctgtgccgg
     2101 gataatagca actcttagtg agcaaataaa tgccgatacc gcgtcgtttg ttgaagtgat
     2161 aatacctgac gttttaaaca catatgactt aatagtctgg gcagaaaaaa acagccatga
     2221 cattgttact cagagaaaag atgca
//
LOCUS       DASCNS010000165         5108 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001281170, whole genome shotgun sequence.
ACCESSION   DASCNS010000165 DASCNS010000000
VERSION     DASCNS010000165.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001281170"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(145..1653)
                     /locus_tag="QF838_05950"
     CDS             complement(145..1653)
                     /locus_tag="QF838_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60257.1"
                     /translation="MIKGDRIEAVGRFEGIEGSRIIEAGGIAASPGFIDTHTHSDGKL
                     LENPQHANSLRQGVTTEILGQDGLSYAPLSKENYLANRRYLSGILGLPSAELDMSSVA
                     SFKSHYHNKVSINTAYCIAHGAIRLEAVGFEDAPLTGTHLEKAKALIRQGMEDGAVGL
                     ATGMSYFPNAWSDTQELIELCKVVAEYQGVYVTHLRDKNTDRAFGGGGVPEALEIGRR
                     SGVKVHFSHFRTNEGNAGQVQAQTELIDKAIRDGVDVSLELYPYPTGSTFPLSFLPSY
                     AHEGGSDAVMDRLADSAERKRLSDYLDNDYSRPIDDAVFSYVPLHPELEGMSLPQIAA
                     SRGKSLGETLCEILLENEGQVGYWASPPVSVSIWDQINRDAIELLSRPDYMVGSDSIP
                     LGSNPHPRAYGTFPRIIGRFQRKYNMMKLEETIQRLTDNPARRFGLESRGRIKPGYFA
                     DVVLFDPNSVIDNSTYDDPTQYPTGIPFVIVNGTLAVDNEVCTGKFTGRAIP"
     gene            complement(2345..3286)
                     /locus_tag="QF838_05955"
     CDS             complement(2345..3286)
                     /locus_tag="QF838_05955"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60258.1"
                     /translation="MDEINIGFIGLGNMGHHMCLALIQKNYNVTVHDINREAALPALE
                     LGAAWADTPRELAQKSDIIFTSLPGPTEVEAVATGEDGIIDGITSDSVWADLSTSSPE
                     LMRRLQETFSSKGASILDAPVSGRRSDENGVTLNGVLSIMVGGHENIYLELKPIFESF
                     GNKVTYTGEIGSATICKLANNMFQYGLERLLVESLTLGVKAGVPAEILMQCIRNGAGG
                     SGRILNVSMPDTYLQGKFDGGTGSESTFPISRKDMALALELGRELNVPLQIATGTYND
                     MTAALNRKEWADLNYRVYHLLQEERAGNVEVRIPTED"
     gene            3409..4251
                     /locus_tag="QF838_05960"
     CDS             3409..4251
                     /locus_tag="QF838_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60259.1"
                     /translation="MNSTNRPVAVVIGATSKWQSDGRNTRLVHGDNLDDSDLPMGVRW
                     GVGGAIAQKFAQKGFFVVLTTRTASNASKLQEAIVDQGGESMIVELDLVSSDSISTAF
                     SQIREQAGDPEVLIYNAGYLEGRDLPPEKELLEHIPLEMLETAQHISVRGPFLVAKEV
                     MPAMRERGQGSFLFSNNSQSLRGRKRMTGQSLYYPRVMMRTLAQVLTEEYSEFGVHVA
                     NVVIDGTIDSPGTRAMPRSQQNPELIINPVKIAEAFYYLHTQDKSCWTHELQLTPYPT
                     KPSF"
     gene            4279..4512
                     /locus_tag="QF838_05965"
     CDS             4279..4512
                     /locus_tag="QF838_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60260.1"
                     /translation="MPFYERGPVSIYYEEVGAGFPLLIIPGGGLNSSIASLDTSVPFN
                     PMERYKNDFRCISADLRNADSGQSTGPLEIDRP"
     gene            4600..5034
                     /locus_tag="QF838_05970"
     CDS             4600..5034
                     /locus_tag="QF838_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60261.1"
                     /translation="MIHNFLRLAPDRIPAATMMQPSGFTSEYPDIFYQNNTERWGPPL
                     CEKAPEITMDKVHDFLTNMYTNRADFVFTVSRDFVRSLQTPLFIAPDNVPAHPYGTAM
                     EVAELAPKAETSIFPWKDSQEHIDEVVEHAGRFLKKHELKTG"
BASE COUNT         1447 a         1198 c         1022 g         1441 t
ORIGIN      
        1 aaattccatt aacgtacctg ccaagcaatg cgttaagggt gaaggtaaag gcggtaggta
       61 acctaattta gactaaaacg aatctttgaa tttagttttt gtcgctaatt gccaatgacc
      121 acacactaaa agataggaaa taaatcacgg aatagctcga ccagtgaatt tacccgtgca
      181 tacctcgtta tccacagcta aagttccatt cacaatgaca aatggaatac cagtcggata
      241 ttgcgtagga tcatcatatg tggagttatc aatcacggaa ttagggtcga acaaaacgac
      301 gtctgcaaaa tatcctggct tgatacgacc tcttgattct aagccaaacc tgcgggcagg
      361 gttatcagtc agcctctgaa tagtctcctc aagtttcatc atattatatt ttcgctgaaa
      421 tctacctatg attctgggga aggtaccata ggctctggga tgtggattgc tacctaatgg
      481 aatcgaatca ctacctacca tgtaatcagg tcgagacagt aattcaatag catcgcggtt
      541 tatttgatcc catatcgaca cactgaccgg aggagaagcc caatatccaa cctgaccttc
      601 gttttctaaa agaatttcac aaagagtttc accgagactc ttacctcttg aggcggcaat
      661 ttgtggcaag gacattcctt ccagttcagg atggagtggc acatacgaaa atacagcatc
      721 gtctatcggc cgtgagtagt cattatcgag ataatccgac aaccttttcc tttcagcaga
      781 atcagccagc ctgtccataa ccgcgtctga gcctccttca tgggcataac ttggcaaaaa
      841 gctcaacgga aaggtgctgc ctgtagggta tggataaagc tcaagcgata cgtcgactcc
      901 atctctgata gctttatcaa ttagttctgt ttgagcttgc acctgacctg cattaccttc
      961 atttgtccga aaatgagaga aatgcacttt cacaccagat cttcgaccaa tctctaaggc
     1021 ctcgggtaca cctccacctc caaaggctct atctgtattc ttatccctta agtgagtcac
     1081 ataaacccct tgatactcag caacaacctt gcaaagttct attagttctt gagtgtcact
     1141 ccaagcgttg ggaaaatacg acatgccagt tgccaatcca acagcaccat cttccatccc
     1201 ttgtcttatg agagcttttg ctttctccaa atgagttccg gttaggggag catcttcaaa
     1261 ccccacggcc tcaaggcgaa tcgccccatg ggcaatacag taggccgtat ttatagaaac
     1321 tttattgtga taatgagact taaaacttgc taccgatgac atatccagtt cagcggaggg
     1381 aagtcctaaa ataccagaaa gataccttct attagcgaga tagttttcct tagacaacgg
     1441 ggcgtatgac aacccgtctt gacccagtat ctcggtagtt actccttggc gcagactatt
     1501 ggcatgctgt gggttttcaa gaagttttcc gtcagagtga gtatgggtat cgatgaaacc
     1561 aggagaagcc gcaataccac ctgcttctat aatccttgat ccctctatgc cctcaaacct
     1621 gccgactgcc tctatccgat cacctttgat caatatatcc cccgaactcg ggggggaccc
     1681 tgtgccatca taaattgtgc aatttcgaat taagatgttt gaaatttgaa tcgacctctg
     1741 taacttattt aagtgcttgt ctagcggcat tatacagctt gttttattgc ccatagggcc
     1801 aatggtgaac tcgagaatat ctgacaggaa attgatttct aactcaccaa ctgtttcgcc
     1861 cgcaatgctc cagttaaagg caatggtgtt gtactcacta gtccctcaaa tctgcccgaa
     1921 tttaatcaaa tgttttccaa tgtcacagct aacatatccg aatatgtttg gcaggaattc
     1981 atatcagaat ttggcatagg gacatagtca cctccggatg ggttatacct aataaaaatg
     2041 attaaatata ttaatttaat gatgtatagt gccaaaaata tatattcaat gcggaggtat
     2101 tattatgatt tttccttctc ttcttcaagc ataaccacta cagtcgctcc gacgtctggg
     2161 atatcttgtg ctgtgacaca tcccacaatg atcattgtca gaaggaccat agcaacaacg
     2221 atcagatata accacttctt gataccaaga aagccctcga ggttatcttt tacttaatat
     2281 ttccggtttc ccacaacggc gaatttcaat cgtcttagga aaataattcg gaatattaac
     2341 ctatttagtc ctcagtagga attctaacct cgacgttacc cgccctttct tcttggagga
     2401 ggtgataaac cctgtaattt aaatcagccc attcctttct atttaaggct gccgtcatgt
     2461 cattgtaggt cccagtagct atctgaagag gtacgttaag ttcacgtccc aattccaacg
     2521 ccaatgccat gtcctttcta gagataggga aagttgattc cgatccggtt cctccgtcaa
     2581 atttcccctg caaatacgta tctggcatgg aaacattgag gattcgtccg ctgccaccgg
     2641 caccatttct tatgcactgc atgagtatct ctgcaggcac tcctgccttt acaccaaggg
     2701 tgagtgattc aactaacaaa cgttccaagc catattggaa catgttattt gccagtttac
     2761 agatcgttgc agaacctatt tccccagtgt acgtgacctt attgccaaaa gattcaaaaa
     2821 taggtttgag ctccaaatat atgttctcat gtccacccac catgattgat aaaaccccat
     2881 ttaacgtcac accgttttca tctgatcggc gtccacttac aggagcatct aatattgaag
     2941 cacccttaga agaaaatgtt tcctgcaatc tcctcattaa ttcaggagaa cttgtactaa
     3001 ggtctgccca gactgagtca ctagttatcc cgtcgattat cccatcctcg ccagtggcaa
     3061 cagcttcaac ttcagtggga cctggtaaag aagtaaatat tatgtcactt ttttgtgcca
     3121 gctcccttgg agtatctgcc catgcagccc ctaattccaa tgcaggtaaa gcagcttctc
     3181 tgtttatatc gtgcacagtt acgttgtagt tcttttgtat caaagcaagg cacatatgat
     3241 gtcccatatt gccaagacct ataaacccga tatttatttc atccatttat cgttactcct
     3301 ttgcgttcag atacaatgcc ttctgggcaa gtacacgata tcacctagta gggataccct
     3361 gactaacgaa gtcagtgcta attaacattt acaccaaggg ggcctgacat gaactcaacc
     3421 aatagacctg tagcagttgt cataggagcc acatccaaat ggcaatctga cggacgaaat
     3481 acaagattgg ttcatggtga caatcttgat gatagtgatt tacctatggg ggttcgttgg
     3541 ggagtaggag gtgccatagc gcaaaaattc gctcagaaag gttttttcgt agtactcact
     3601 acgcgtaccg cgtcaaatgc ttctaaactt caagaggcta tcgttgacca gggtggcgaa
     3661 agcatgattg tcgaactaga tttggtatca agcgactcga tatcaactgc gttttcccaa
     3721 atacgggaac aggccggaga ccctgaagtt ctaatctaca acgcaggcta tcttgaaggt
     3781 cgagacctcc cgcctgaaaa agaattgctt gagcatattc cacttgaaat gcttgaaacg
     3841 gcgcaacaca tttcagttag aggaccattt ttagtagcta aagaagtgat gccagcaatg
     3901 cgagaaagag gtcaaggttc cttcttgttt tccaataatt ctcaatcttt gcggggtagg
     3961 aaacgaatga caggacagtc actatattac cctagagtga tgatgcgcac tttagctcaa
     4021 gtgctcactg aagagtattc cgaatttgga gttcatgtag ccaatgtggt cattgacggt
     4081 actatcgact ctcctggaac aagagctatg ccgcgatctc agcaaaaccc ggaattaata
     4141 ataaatccag tcaaaattgc tgaagctttt tactaccttc atactcaaga taaatcgtgt
     4201 tggactcatg aactccagct caccccatat cctaccaagc caagtttcta gaaattcttt
     4261 caaatacagg gggaatccat gccgttttac gaaagaggtc cggtcagtat ctactacgaa
     4321 gaagtagggg ctggttttcc tttattaatt atccccggtg gaggtcttaa ttcttcgatc
     4381 gcttccctgg atacttctgt cccatttaat cctatggaaa ggtataaaaa tgactttcgt
     4441 tgcatatctg ctgatttaag aaacgctgac tccggacaat caacaggacc attagaaatt
     4501 gaccgcccct gagatgctta ctcagacgac cgactcgggc ttatggatca tcttggtgta
     4561 aaagaatttc tggtcatggg cttctgtatt ggtgggccaa tgattcacaa ttttttgcga
     4621 ctagctcctg accgaatacc ggcagcgaca atgatgcagc caagcggttt cacttctgag
     4681 tatccagata ttttctatca aaacaacaca gaacggtggg gaccaccttt atgtgaaaaa
     4741 gctcccgaaa tcaccatgga taaagttcac gactttctca caaatatgta caccaaccga
     4801 gcagattttg tcttcacagt ctcccgtgat tttgtgcgtt cacttcaaac accacttttt
     4861 atagcgcctg ataacgttcc agcacatcca tatgggaccg ctatggaagt tgccgaactt
     4921 gcacctaaag ctgagacgtc aattttcccg tggaaggatt cgcaagaaca catcgatgag
     4981 gtggtagaac atgcaggtcg tttcctcaag aagcatgagc ttaagacagg gtaatgattg
     5041 gttgggaagt catttccttg ttataaatat aatcgtactt cccatttgat ctgactacac
     5101 gctctcag
//
LOCUS       DASCNS010000166         6525 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001283670, whole genome shotgun sequence.
ACCESSION   DASCNS010000166 DASCNS010000000
VERSION     DASCNS010000166.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001283670"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="QF838_05975"
     CDS             complement(<1..911)
                     /locus_tag="QF838_05975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HJO60262.1"
                     /translation="MSEDFSTYVTVEQIIQRARKNLAQGPWDYLAGASESETTMRRNR
                     LAFDKVAFRPRVLADVSSVNPSTTFIGHKIRIPVLLAPIGSLQVFIPEGGAAATAAAG
                     EFGTMHVVSSVTQPSLEETSATTDFPKSYQLYIHGDWEWTKEMISRAKSADYKGFCIT
                     VDTANYSRRERPMLSGWTPVSRRTPVDPKWQASVTWDSIDKIKSEAGLPFMLKGIATA
                     EDAKIAIEHGVDVVWISNHGGRQLDHGQGTMEMLPEIVEAVDGKAEIVLDGGVQRGSD
                     VVKAVAMGATAVAIGKMQGWGLAAGGVP"
     gene            1037..1951
                     /locus_tag="QF838_05980"
     CDS             1037..1951
                     /locus_tag="QF838_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HJO60263.1"
                     /translation="MTTAIRICSLLPSTTEIVCTLGLKENLVGITHGCDYPRDISEIQ
                     VVTKSLIDHSGSSSLEINRHISEALHSGSGIYAIDNESLEFTNPDLILTQEICEVCAV
                     SYSQVEESVRTLSGNQTVLSFEPSNIEGILDSIVQIGKQTNTEPNAETLVSEAYGRID
                     TVKSKCANSPRKPRVLGLEWLEPPFIGDHWVPEMIQIAGGTPALGDQEAPSMEITWGQ
                     ALASSPEIIVLMVCRFDLKRTVEEFSLLGESAFWKKYDGDIYAVDGSAYFSRPGPRTI
                     DGIEILSEIIHPNIFQRKTEPNAWMKVN"
     gene            complement(1948..3099)
                     /locus_tag="QF838_05985"
     CDS             complement(1948..3099)
                     /locus_tag="QF838_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HJO60264.1"
                     /translation="MPKNLPSLLVDIKPGVIDLGWGHPSSRLHPFDEVSEASSRMFSK
                     GDNSALQYGATQGYGPFLESLASFLSDQKAYLPDVDPHNLFLTAGASQALDLAATMYA
                     DSGDTVVVEEPTYFVIEKIFREHSLNIVGIPTDEDGLDTDVLQKGLERGLSPKFLYTI
                     PTFQNPTASSLTEQRREHLVELANDYNFQIFADEVYQLIYFDQKPPRPMMYFDSGNRV
                     ISFGSFSKILAPGLRTGWIHSSREVVGKLSDAALTFSGGGFNHYASALIKEVIDLGHL
                     ESNVKKLQIEYISRAREMDKSLKEYFGTSVSYSFPKGGYYFWLKFPEGFDSDSFLHHA
                     EENGVSFRPGNAFSESGKFSRYLRLTYTLFESDEIREGVKRLAEAYKNT"
     gene            complement(3112..3783)
                     /locus_tag="QF838_05990"
     CDS             complement(3112..3783)
                     /locus_tag="QF838_05990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO60265.1"
                     /translation="MSLQILKKECPLCLSVHVDLLFTSARKKLERAYLSCDNCGLVFV
                     PSQDQLTVDEQRDRYLEHNNSPTDPGYQKFLSRLKNQMTPHLIPGQTGLDYGSGPGPT
                     LSLMLESEGYKMEIFDPIFAEYNENLDHLYDFVVSTETAEHFTKPNEDFQILHDLVRP
                     GGLIGIMTSILYEGINFEDWYYRLDPTHISFYRPKTMEFVADQWNLESSSPAKDIYIF
                     RKPAG"
     gene            complement(3804..3880)
                     /locus_tag="QF838_05995"
     tRNA            complement(3804..3880)
                     /locus_tag="QF838_05995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3844..3846),aa:Glu,seq:ctc)
     gene            4458..4925
                     /locus_tag="QF838_06000"
     CDS             4458..4925
                     /locus_tag="QF838_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60266.1"
                     /translation="MRKLIVLSMVLIAILVGCSSEPEKTFQNPAEVIKALDKHNVTEC
                     NDLGAEAFLANESVICFIELGGGELKDVMFNISTYADIKQIDLFKDKCKKNDVGVFPD
                     CSKTLEHTFYHGNVMMSAFEMYFSGTTKESTLGYDMRHVSKEYIDSILEDLAD"
     gene            complement(5140..5616)
                     /locus_tag="QF838_06005"
     CDS             complement(5140..5616)
                     /locus_tag="QF838_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HJO60267.1"
                     /translation="MDQNIKKLALRQISYGLYIMSASDGTNHSAGTINWLSQASFQPP
                     MVMVAVKADSRLYELVEKTKVFAINILSDSQKQIAEDFFRPTTFEDNKLNGHSFIAGD
                     KGAPILDEIYAHFECTVSGKMELGDHVIFAGEVVSVGQRGDGKPLEMSTTGWFYGG"
     gene            complement(5772..6071)
                     /locus_tag="QF838_06010"
     CDS             complement(5772..6071)
                     /locus_tag="QF838_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60268.1"
                     /translation="MSITFLGFYRPYFREADVETVRGSGQFLPEMRDKVKAFPSSLPD
                     TCRLIGSWGVTGGEAPGVMVVEADSFSDIQFINTYYAGWLNFDWHPTNTGGVPRS"
     gene            complement(6304..>6525)
                     /locus_tag="QF838_06015"
     CDS             complement(6304..>6525)
                     /locus_tag="QF838_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine reductase"
                     /protein_id="HJO60269.1"
                     /translation="ANITFYPGQSVIELKSLKQYLFQFRDTRISYERIINTIYDDLID
                     IYSPKRIRLVVEFNVRGGKEEFNYWAKPE"
BASE COUNT         1888 a         1406 c         1343 g         1888 t
ORIGIN      
        1 ggaacgcctc cagctgctag tccccatcct tgcatcttac cgattgcgac agctgtggct
       61 cccatagcca ccgccttcac aacatcagaa cctctttgca ccccaccatc caacacgatc
      121 tcggccttac catcaaccgc ctcaactatc tccggaagca tttccatcgt tccttgtcca
      181 tgatcaagct gcctaccccc gtgattagaa atccacacca catcgacccc atgttctatt
      241 gctattttgg catcttctgc agttgcgatg ccttttagca tgaaaggtaa ccctgcctct
      301 gactttatct tatctatact gtcccatgta actgaagctt gccatttcgg atctacagga
      361 gttcttctgg aaaccggagt ccatccgcta agcatcggtc tctctctacg actgtaattt
      421 gcagtatcca cggtgatgca aaacccttta taatcggccg acttcgctct agatatcatc
      481 tctttagtcc attcccagtc tccatggatg taaagctgat acgacttggg aaaatcagtc
      541 gtagcagatg tctcttcaag ggatggttga gttacagaac taaccacatg catggtgcca
      601 aattctcccg ctgccgcagt ggcagctgcc ccgccttctg gaatgaaaac ctgaagtgac
      661 ccaatgggag ccaataagac cggtatccgt attttatggc ctatgaaggt tgttgaaggg
      721 ttaacactag aaacatcagc cagaacccta ggccggaagg ctaccttatc aaaagccagc
      781 ctatttctcc tcatagtggt ctcagattca gaagcacctg ctaaataatc ccaaggccct
      841 tgagccaaat tctttctagc tctctggata atttgttcaa ctgttacgta tgtagaaaaa
      901 tcttcagaca aggcatccct ccacctaagt gattactttt taagctcaag aagctgctat
      961 aaattgcagt ggtagtatat ccaagctgga gaaaaagtta agccccattg agctatttcc
     1021 ttaaaatgag gcccaagtga cgactgcaat aagaatctgt tctctacttc cgagcaccac
     1081 tgaaattgtt tgtacgcttg gtttgaagga aaatctagtt ggcataactc atggatgcga
     1141 ttaccctcgc gacattagcg aaattcaagt agtgaccaag agtcttatcg accattcagg
     1201 gagcagctct ctggaaataa atcggcatat atctgaagct ctccattctg gttcaggcat
     1261 ttatgcgata gacaacgaat ccttagaatt taccaatcct gatctaatac taacccaaga
     1321 gatttgcgag gtttgcgctg tttcctacag ccaggtagaa gaaagtgtta ggacattaag
     1381 tggaaaccaa acagtccttt catttgaacc ctccaatatt gagggtatct tggattccat
     1441 agtacaaata ggaaaacaaa ccaatactga accaaatgct gaaacccttg taagtgaagc
     1501 ttatggaagg atcgatactg taaagtcgaa atgtgccaac tctcccagaa aaccgagagt
     1561 attgggattg gagtggttgg aacctccctt cattggagat cattgggttc cagagatgat
     1621 tcaaattgca ggcggcaccc cggctttagg agatcaggaa gcaccatcca tggagatcac
     1681 atgggggcaa gctttggcat cttcaccaga aataatagtg ttgatggttt gcagattcga
     1741 tttaaagaga acagtagagg aatttagttt actgggcgaa tccgcatttt ggaagaaata
     1801 tgacggtgac atatatgcag tagatgggtc cgcatatttt agtaggccgg gcccaagaac
     1861 catagatgga attgaaattc tcagcgagat catccaccca aatattttcc agaggaaaac
     1921 agaaccaaat gcatggatga aggtgaatta ggtgtttttg taggcctctg ccaaacgctt
     1981 caccccctca cggatttcat ctgactcaaa aagagtgtaa gttagcctaa gatatctgct
     2041 gaacttacca gattctgaga aagcatttcc aggcctaaag gaaacaccgt tctcttccgc
     2101 atggtgcaag aatgaatctg aatcaaaccc ttctggaaat tttaaccaaa aataataccc
     2161 cccttttgga aaagaatatg aaacggatgt tccaaagtac tctttcaaac ttttatccat
     2221 ttctcgagcc ctcgaaatat attcgatctg cagttttttg acattcgact ctaaatggcc
     2281 caaatctatc acctctttaa tcaaggcaga agcataatga ttgaatcccc caccactaaa
     2341 agttagtgca gcatctgaca atttacctac gacttctcta gatgagtgta tccagcctgt
     2401 tcttaatcct ggggccaata ttttggagaa tgacccaaaa gatatgactc gattacctga
     2461 gtcgaagtac atcataggtc tcgggggctt ttgatcaaaa taaattagtt gataaacctc
     2521 gtcggcaaat atttggaaat tatagtcgtt agccaactcg acaagatgct ccctcctttg
     2581 ttcagttaga gaagaggccg tgggattctg aaaagtggga atcgtgtaca aaaatttagg
     2641 acttaaacct ctttctaaac ctttttgtag cacatctgta tctagtccat cttcgtcagt
     2701 aggaattcca acaatattaa gagagtgctc ccgaaaaatt ttttcgatca cgaaataggt
     2761 tggctcttcc actacaaccg tatccccaga atctgcgtac atagtcgccg ccaaatccaa
     2821 agcctgtgaa gctccggcag taagaaacaa attatgagga tccacatctg gaagatatgc
     2881 cttctgatct gacaaaaatg atgcgaggct ttctaaaaag ggcccatagc cctgagttgc
     2941 cccatactgt aaggcagagt tatcaccctt tgagaacatt ctggatgaag cttcgcttac
     3001 ctcatcgaaa ggatgcagtc gtgatgaagg gtgtccccag ccaaggtcta taaccccagg
     3061 cttaatatca acgagtagag atggtaaatt tttaggcatg ttatatgagg actatcctgc
     3121 aggttttcga aatatgtata tatccttcgc tggtgatgat gattccaggt tccattggtc
     3181 cgctacaaac tccatagttt taggtctata aaaacttata tgtgtaggat ccagcctata
     3241 gtaccagtcc tcaaaattaa ttccctcgta cagaatagaa gtcataattc caatcagccc
     3301 ccccggtcta actaggtcgt gtaaaatctg gaaatcttca tttggttttg taaaatgctc
     3361 cgctgtttcc gtactaacta caaaatcata taaatgatcc aagttttcgt tatattcagc
     3421 aaaaatagga tcgaaaatct ccattttgta accctctgat tccagcatga gggacaaggt
     3481 agggcctggc ccagaaccat agtccaaacc agtttggccc ggtattagat gtggagtcat
     3541 ttggtttttc agtctactaa gaaacttttg atagccagga tccgtaggtg aattattgtg
     3601 ttccaagtac cgatcacgct gttcatctac agttaattgg tcctgacttg ggacaaatac
     3661 cagcccgcaa ttatcgcaag ataagtaggc acgttccaat tttttcctgg cacttgtaaa
     3721 taaaagatct acgtgaactg acaggcacag aggacattct tttttaagaa tctgaagact
     3781 caccttttct tccaacttcc acgtggtagc gcatacggga ttcgaacccg tgatctccgc
     3841 cttgagaggg cggtgtccta ggccgctaga cgaatgcgcc acgtataaat cagcggtatg
     3901 acaagaacat tttacttcat gttttaccga agtgtttgca aaagtgtttg caggtaaagc
     3961 gtcatatctg gctttaatct ctgtattcca cggtctcctt ccatttatca tttggctcag
     4021 atagggagcg ctcaccccta ttatctttgc cgtttttcta agactcattg ttgtcctcta
     4081 ttcctataat cctgacattc aagttttcaa gctggtattt cgtgactaca agcgatgctt
     4141 tcttggggga attaagccat atccaattca aatgtggggc attgtaaccc tattttcgta
     4201 ttcaaaatta aatagagata agcagattaa ttattcgaaa atttctggca aagtaggctt
     4261 ccatatatgg aaggacgaaa ggactaaagg gtattcaatc gtgtgtggag cgatgggctc
     4321 acagtataga tatgcggaaa gacgaacccc cttaatccat tcctgctggt accattagat
     4381 ggtcatatca gtccctccac tggggctgga gtgaccattg ttcttaagct ttccgagttg
     4441 ttataaaaga ggtagtaatg aggaagttga ttgttctgtc gatggtatta atagccatac
     4501 ttgtaggctg tagttcggag ccagagaaga ccttccaaaa tcctgctgaa gttattaagg
     4561 cacttgataa gcataatgtt actgaatgta atgatttggg tgccgaagcg ttccttgcca
     4621 acgaatcggt aatttgtttt atagaattag gtggaggtga attaaaggat gttatgttta
     4681 atatttctac atatgctgat atcaaacaga tagatttatt taaagataag tgcaagaaga
     4741 atgatgttgg cgtttttcct gactgtagta agacgttgga acacactttt tatcatggca
     4801 atgtaatgat gtctgctttc gaaatgtatt tcagtggtac aactaaggag tcgacccttg
     4861 gatatgatat gcgccatgtt tcaaaggagt atatagattc tattctggaa gatttagcag
     4921 attagaccga gattattcaa aatcttgtgt ggacaatggc taacagtagt tgaggggcga
     4981 agggactaag gggtattcaa aggtgtgtgt ggcggtaggc tcacagtata gatatgcgga
     5041 aagacgaagc cccttgtaat aatggaatta cgtgcgagtt attgtgtagg tagcgccgct
     5101 gggacgctac ccgtcatgtt tcttaaacct aaacagagat taccctccgt agaaccaacc
     5161 tgtcgttgac atttcaagag gttttccatc tcccctttgc cccacggaaa caacctctcc
     5221 cgcaaatatg acatggtctc ctagttccat tttgcctgat actgtgcact caaaatgggc
     5281 atatatctca tctaatatag gcgctccctt gtctccagct ataaaagaat ggccatttaa
     5341 tttgttatcc tcaaacgtgg tgggcctgaa aaaatcctca gcaatttgtt tttgtgaatc
     5401 agaaagaata tttatagcaa ataccttggt tttttccacc agttcataga gcctgctgtc
     5461 tgccttaacg gctaccatca ccataggtgg ctgaaaggag gcttgtgata gccagtttat
     5521 tgttccggca ctatgattgg taccgtctga tgcagacata atatacagcc cgtacgaaat
     5581 ttggcgtagt gccaatttct taatgttttg gtccatatta caacctccag aatttctgtt
     5641 tataacaata tctatttaat tctggtaatt ctgaatcata aatccagaaa ggtctagaac
     5701 tgtgcttggg ttatgctctc tgaatagaga tagctaggga tgaagccctc ttcatgctgt
     5761 aatactttgt cctaacttct tggtacacca cctgtgtttg ttggatgcca gtcgaagtta
     5821 agccaacccg cataatacgt gttaataaat tgaatatctg aaaaactgtc cgcctccacg
     5881 accattacgc caggcgcctc gcctccagta actccccacg aacctataag tctacaagta
     5941 tcagggaggg aagaaggaaa agctttaact ttatctctca tttcaggtaa gaattggcca
     6001 cttcccctaa ccgtttcaac atctgcctca cggaagtagg gtcgatagaa tcccaaaaat
     6061 gtaatagaca tatataaatt ctccaattta ggtttatttt ggagaaaata gctaaatttt
     6121 aataatctgt caaacaaggt ttgttttgat gcggaagctt gataaggccg gagtatttga
     6181 aatcatgtgg aacactatgc cccctgaata gggatgggtg gggagcaacc ccttattgac
     6241 gagtattcat tttattaaag gggttgaaaa cagctggtga ataaacaccc tacagcaggt
     6301 ctattattca ggtttagccc agtagttaaa ttcttcttta cccccacgca cattgaattc
     6361 cacaaccaag cgaattcgtt taggggagta aatatcaatt aagtcgtcat aaatagtgtt
     6421 gataattcgt tcatatgaaa tacgtgtatc tctaaattgg aataaatatt gttttagtga
     6481 ttttaattca ataacacttt ggcccggata aaatgttatg ttggc
//
LOCUS       DASCNS010000167         2275 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001286484, whole genome shotgun sequence.
ACCESSION   DASCNS010000167 DASCNS010000000
VERSION     DASCNS010000167.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001286484"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="QF838_06020"
     CDS             complement(<1..1283)
                     /locus_tag="QF838_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60270.1"
                     /translation="MHDLLIQGGTVVTASGPRKLGILVDDGRITSLSTEEIDANDANK
                     IIDAEGMIVVPGGIEPHAHIGGPRQPERSGAEVVSKAALLGGTTTVLDFATQIPGHDL
                     YHALEEAEERWSGNAYTDYAYHPILTNGADEDSISQIKQLTKEGIASFKIFTTSIRPP
                     SPQMQNNHTDFGRLGTIMEQVADAGSILLVHSEDDEMVHYNYDRLKETDEWEWWNMHR
                     VHTKQSEDVSFRRVSRLSELKECPIYFVHVSAQEGVEEVYRCRAQGLPIYGETLHNYA
                     CFTFQDYRSKNGMKYHTYPSLKGEEDRDSLWGGLMEGTLSTIATDLVSTTWEEKIKFR
                     TVADVTGGHNGIETRMGVAYTEGVVKRGMSLERFVDITSTNAAKILGLYPRKGVITPG
                     SDADITIMDPTVDKDLSLDDLHLEDYSIWEGYRVKG"
     gene            complement(1406..>2275)
                     /locus_tag="QF838_06025"
     CDS             complement(1406..>2275)
                     /locus_tag="QF838_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HJO60271.1"
                     /translation="SIKVLKNGGTAMDAIVAGIRMVESNLSDHSVGRGGLPNLLGQVE
                     LDASVMDGQTLRAGAVAGLKNFEHPISIAHKVMEELPHVMLVDRGAAKFAKEMGFRPR
                     NLLTSAAKRKWVEGLDERGEGLQGMDKYLDNLRKYSSNLPTAKHLGTVNFIARDRKGN
                     IASGVSTSGLGWKYPGRVGDSPIIGAGNFADNRYGSAACTGRGELSMRACTARSVVMY
                     LQSGLTLLDAGQRAMEDLKDYQDSFGTYMNIVCMDKDGQTAAFSFAESTIYNVQNEEM
                     DRYEEIPRKKINI"
BASE COUNT          580 a          587 c          404 g          704 t
ORIGIN      
        1 cctttcaccc gatacccttc ccaaatactg taatcctcca gatgcaaatc atcgagggac
       61 aaatccttgt ccactgttgg atccataatg gtgatatccg catcacttcc cggggttatc
      121 acacctttcc ttggatacaa accaagtatt ttcgcggcat tggttgatgt tatatctaca
      181 aacctttcta aagacattcc tcttttcaca accccctccg tatatgcaac acccatcctg
      241 gtttcaatac cattatggcc acctgttaca tctgcaacgg tcctaaattt gattttttcc
      301 tcccaggtcg tggagaccaa atccgtggct atagttgaaa gagttccttc cattagtcct
      361 ccccacaagg aatcgcgatc ttcttcacct tttaaggaag gatatgtgtg atatttcata
      421 ccatttttac tgcgatagtc ttgaaatgta aaacaggcat aattatgtaa agtttctcca
      481 taaatcggta acccttgagc ccgacatcta tagacctctt ctactccctc ttgggcgcta
      541 acatgtacaa aatagatagg gcattctttt aattctgaca gtcgtgaaac ccgtctaaat
      601 gagacatctt ctgactgttt agtatggact ctatgcatat tccaccattc ccattcatca
      661 gtctctttga gccggtcata gttgtaatga accatctcat catcttctga atgcactagt
      721 aagatcgatc ctgcatcagc cacctgttcc ataattgtcc ccaatcttcc aaaatcggta
      781 tggttatttt gcatctgagg gctcgggggc cgaatactag tcgtaaatat tttgaagctt
      841 gctattcctt cctttgtgag ttgcttgatc tgggatatgc tgtcctcatc cgcaccgttg
      901 gtcaaaattg gatggtaggc ataatctgta taagcattac cagaccatcg ctcttcagcc
      961 tcttccaagg cgtgatataa atcatgccca ggtatttgag ttgcaaaatc cagtactgtt
     1021 gttgtgcccc ctaataaggc cgctttggat accacttccg caccagacct ttcaggctgg
     1081 cgtggccctc ctatatgagc gtgtggttct atccctcctg gaactacgat cataccttcg
     1141 gcatcaataa tcttgttagc gtcatttgca tctatttctt cagtcgataa agaagtgatc
     1201 cgcccgtcat cgacaaggat acccaacttc ctcgggccgg aggctgttac aacagtgcct
     1261 ccttgaatta atagatcatg cacaatctgc ctcctttcat acttaaccca ctgaataggc
     1321 gcatattaac actaagaggt ctgaggttcc tttctaagtc ctcgttgcca tcgtgaagtt
     1381 tgttgatgaa tcggatccat tggaatcaaa tgttgatctt tttcctaggg atttcctcat
     1441 atcgatccat ttcctcattt tgtacgttat atatggttga ttcagcaaag ctgaaggcag
     1501 cggtttgacc gtctttatcc atacatacta tgttcatata tgtaccaaaa gagtcttgat
     1561 aatctttcag atcttccatg gccctttgtc cagcatccaa cagagtcaaa ccagattgaa
     1621 gatacatcac cacagaacga gcagtacaag ccctcataga taattcacct ctacccgtac
     1681 aagcagctga cccatatctg ttgtctgcga aattgccagc ccctattatt ggcgagtctc
     1741 ctactcgacc tggatatttc caacctaagc cactggttga taccccagaa gctatattcc
     1801 ctttcctatc tcgagctata aagttaactg ttcccaggtg tttagctgtg ggcaaatttg
     1861 agctatattt ccttaaatta tcgagatact tatccattcc ctgtaaccct tctcctcttt
     1921 cgtcgagacc ttccacccat ttcctttttg ctgcagaggt gagcaaattc ctgggacgaa
     1981 accccatttc tttcgcaaat ttggcagccc ccctgtctac aagcatgaca tgaggaagtt
     2041 cttccattac cttgtgggca atagaaattg gatgttcaaa attcttaagt cctgctacag
     2101 caccggcacg taaggtttgt ccatccatta cggaggcatc taattcgact tgacccaata
     2161 aatttggtaa tcctcctcgc ccaacagagt ggtctgaaag attactttcc accattctga
     2221 taccggctac aatagcatcc atagcagttc caccattttt taacactttt atgga
//
LOCUS       DASCNS010000168         5077 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001297222, whole genome shotgun sequence.
ACCESSION   DASCNS010000168 DASCNS010000000
VERSION     DASCNS010000168.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001297222"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="QF838_06030"
     CDS             <1..210
                     /locus_tag="QF838_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60272.1"
                     /translation="GVAFIIGGEIKGGMYGQYPSIKEADHLEGDLHFNNDFRSTYSTV
                     VEKWFGLDPVPIVNGHFEQFDFVNA"
     gene            271..1683
                     /locus_tag="QF838_06035"
     CDS             271..1683
                     /locus_tag="QF838_06035"
                     /inference="COORDINATES: protein motif:HMM:NF020390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO60273.1"
                     /translation="MSQDVSLMAHLMRRAGFGATRNELDEYLSDGYEATVDKLLDPGE
                     SDHMPDDLIRRYHVDQSELRQLDGAGAYWLYRMLTTSNPLEEKLALFWHGLFATGYAK
                     LNQARALLNQIDMFRKYGFGSFRDLLVELSRDPAMILWLDNNENHKEAINENYGRELL
                     ELFSMGIGNYSEDDIKECAKAFTGWTLKNAEYMSVRASKDSIWPYGRIAWHYEYRADD
                     HDSGEKKFLGEVGNFNGEDIVDIIVTQKPTARFLSTRLFQYFASDEVDDDGVQVIKAM
                     MDSYFESGFKVSAVLRTLFNSDYFKSEKCRYSRVKGPVESVVGTARLAGSYQEPTLDV
                     HSLWAQTMFMGQGLLAPPTVEGWHEGVEWIDSGSLVERINFAAKELSDPAKEGVQDII
                     RRVTAGYIDLIHAENLVDSCLDFMGPIQVGADTREGLIEFAESQGKLHLKNGKTDEDG
                     SAQVARLIGMIASTREYQLA"
     gene            1710..2732
                     /locus_tag="QF838_06040"
     CDS             1710..2732
                     /locus_tag="QF838_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013595.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO60274.1"
                     /translation="MVITNTTSIKYLKTIGIVNNGFNGRGFANPYDVAIGPGDNIFVL
                     NRCDPARRNAIRVGVCNLDEEYLYEFGYGFGDGDGQMMLPVAMAFDAEGRLLITDEHT
                     NRITIYSDEGEVLGKWGQKGESEGEFNGPAGIATDSSGTVYICDQRNHRVQKYTADGM
                     FLTQWGSQGTGPGEFNLPWGLGLDAEDNVYVADWRNDRVQKFDSNGHHLKTIGDSGVK
                     QEQLIRPSAVSIDQSGNVYVADWGNERLQVFNSEGKVIASLRGEATLSDWAKDFFESN
                     PDEMETREISDLNPDVPDHLNDPYHISSQTEPFFWGPVSVRIDDNNRLYVVESNRHRI
                     QIYEIE"
     gene            complement(2736..3173)
                     /locus_tag="QF838_06045"
     CDS             complement(2736..3173)
                     /locus_tag="QF838_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJO60275.1"
                     /translation="MITGFNHSGFVVRDLDKMVSFYRDDLGLQIEREIDSRAPETGDH
                     TGIPGAHRTLIFVGKGGGHALELVYYIYPDSPSGHLNRNQLGAAHVCFNVENIEELYQ
                     DLTNKGVKFVTPPIMTDTETGGRHGICYCQDPEGNWLEFIQAG"
     gene            3258..3506
                     /locus_tag="QF838_06050"
     CDS             3258..3506
                     /locus_tag="QF838_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60276.1"
                     /translation="MNELLNILKLPYLWGGIGAALGAGLGVNNLSVWLLAVLLGLFFV
                     TMRLTGPPEEGKEGRLFAGGSLLMLGWILAFSIRGIVI"
     gene            3572..4330
                     /locus_tag="QF838_06055"
     CDS             3572..4330
                     /locus_tag="QF838_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014774.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HJO60277.1"
                     /translation="MSTKVSLFATCILNNFYPDVAFSVVRVLSKLGVDVTVRPDQTCC
                     GQPFFNSGHWNDSSKLMRKFVESYSGGQEDIVLPSGSCTSMVRNHYLEVCNDDDIGEI
                     RDVSGRTFEFTEYITRELGIFDLTPFQSAITEKINVTYHESCHLKRELGVSSEPIDLL
                     QSLENVTVMDMDQSEVCCGFGGTFATKYSEISTAMGEEKVANIVKSGADIVTASDMSC
                     LMHIDGLIARKKIPLKSMHISQVIEQSLNMEYQK"
     gene            4327..>5077
                     /locus_tag="QF838_06060"
     CDS             4327..>5077
                     /locus_tag="QF838_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014628.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HJO60278.1"
                     /translation="MKPETKNFNSKSKETLLDPQIQKNLSGLYGGFHSARIKASSDTV
                     DWEELQIKGREIKKHVINNLDYYLELLESNVISSGGNVYFAETAEDATQYVINLAKEK
                     DVKTVVKSKSMLSEEMSLNEELIKHGIDPYETDLGEYIVQLAEETPYHIIAPAIHKSR
                     QEVSELFADKIGTSQTENIETLTLEARDQLRDKFIQADMGITGGNFLVAETGTLALVS
                     NEGNGRMCTSMPPVHVAIVGMEKIIPSMEDLG"
BASE COUNT         1524 a          890 c         1232 g         1431 t
ORIGIN      
        1 ggtgttgcat ttatcatcgg gggtgaaatt aaaggtggaa tgtatggaca gtatccgtct
       61 ataaaagagg cagatcactt ggaaggagat ttacatttca acaatgattt ccggtccact
      121 tattcgaccg ttgttgaaaa atggtttggg ttggaccctg ttccgatagt aaatggtcac
      181 tttgagcagt ttgatttcgt caatgcctaa atagtaagac taataaaacc ggatttggca
      241 ctaaaagata actattcaga ggtgagaaat atgagtcagg acgtttcttt gatggcccat
      301 cttatgcgac gggctgggtt cggtgctact cgtaatgaat tggatgagta tctgagcgat
      361 ggttatgagg ccacggtgga taaactacta gaccctggcg aatccgacca catgccagat
      421 gatctaattc gacgatatca cgttgaccaa tcagaattga gacagttgga tggcgcgggt
      481 gcttattggc tctaccgcat gctgacgaca agtaaccctc ttgaagagaa attagcttta
      541 ttttggcatg gattgtttgc aacaggctac gccaaactaa atcaagcccg ggcattactc
      601 aaccaaattg acatgtttcg gaaatatgga tttggtagtt tccgggattt attagttgaa
      661 ttgtctcggg atcctgcaat gattctgtgg cttgacaata atgagaatca taaagaggcc
      721 atcaacgaga attatggaag ggaattactc gaattattct ccatgggtat tggtaactat
      781 tcagaagacg acattaaaga atgtgctaaa gcttttactg gttggacttt aaaaaatgct
      841 gaatacatgt cagtaagggc aagtaaggat tccatatggc catacggcag aatagcctgg
      901 cattacgaat atcgggcaga cgatcatgat agtggtgaga aaaaattctt aggtgaagtt
      961 gggaatttca acggtgagga cattgttgac ataatcgtaa cccaaaaacc cactgccagg
     1021 tttttatcga caagattgtt tcaatatttc gcatctgatg aagtagatga tgacggagtg
     1081 caggtaataa aagcaatgat ggattcttat ttcgaatccg gatttaaagt ctcagctgtg
     1141 ttgaggactc tttttaactc ggattatttc aaatcagaaa aatgccgata ttccagagtg
     1201 aaaggccctg tagaatctgt tgtaggcaca gctagattag ctggtagcta tcaggaaccc
     1261 actttggacg tgcattctct atgggcacaa acaatgttta tgggacaggg gctgttagca
     1321 cctccaacag ttgaagggtg gcatgaagga gtggagtgga tagatagcgg ttccttggtg
     1381 gagcgcatca atttcgcagc taaggaatta agcgacccag caaaagaggg tgttcaagac
     1441 ataattagga gggtcactgc tgggtatata gatttgatcc atgctgaaaa cttagtagat
     1501 tcatgtttgg attttatggg acccatacaa gtgggagcgg atacaaggga agggttaatt
     1561 gaatttgcgg aatcccaagg caagctacat ctgaaaaacg ggaaaactga tgaagacgga
     1621 tcggcccagg ttgctcggtt gattggaatg attgcctcga cccgagaata ccagctagct
     1681 taaaccacga aaattcagag gtataaatca tggtgattac taatacaacc agtattaagt
     1741 accttaaaac aataggtata gtcaataatg gtttcaatgg ccgtggattc gctaacccat
     1801 acgatgttgc cattggcccc ggggacaata tatttgtatt aaatcgatgt gatcctgcca
     1861 gaagaaacgc gataagggta ggggtctgta atcttgatga agagtatcta tatgaatttg
     1921 gttacggatt cggagatggt gacggtcaaa tgatgttgcc tgtggcgatg gcatttgatg
     1981 cggaagggcg actgcttata acagatgagc acactaacag gataacaatt tattctgatg
     2041 aaggtgaagt tctaggtaaa tggggtcaga aaggtgagtc ggaaggtgaa ttcaacggcc
     2101 cggcggggat agccactgat tcatctggaa cagtttatat ctgtgatcaa cgaaaccata
     2161 gagttcagaa atacactgcg gatggtatgt ttttgactca atggggttct caaggcacag
     2221 gaccaggtga atttaattta ccatggggtc taggcttaga tgcggaagac aatgtttatg
     2281 tagcagactg gagaaatgat agggtccaaa aattcgattc caatgggcac catttgaaaa
     2341 cgattgggga ttcgggagta aagcaagaac aattaataag accgtcggcc gtatcaatag
     2401 atcaaagtgg caatgtatat gtcgccgact ggggtaatga gaggcttcag gtatttaatt
     2461 ctgaggggaa agtgattgct agccttcgag gtgaagcgac tctttctgat tgggctaaag
     2521 atttttttga atcaaatcca gacgaaatgg agacccggga aatatctgac ctgaacccgg
     2581 atgttccgga tcatttaaat gatccatatc atatatcttc ccaaactgaa ccattttttt
     2641 ggggaccggt ttcggtaagg atagatgaca acaatcggtt atatgttgtg gaatctaacc
     2701 gtcatagaat ccagatttat gagattgaat aacatttaac cggcctgtat gaattccagc
     2761 caatttcctt cggggtcttg gcagtagcaa attccatgtc tgcctccagt ttctgtatcg
     2821 gtcattatcg gcggcgttac gaatttgacc cctttatttg tgagatcttg ataaagttcc
     2881 tctatgtttt cgacattgaa acagacgtga gctgctccca gttggttacg gttcagatgt
     2941 ccactgggac tgtccggata aatgtagtac accaattcca gagcgtgtcc accacccttc
     3001 ccaacaaata tcaaagtcct atgggcccct ggaataccgg tatgatcacc agtttcaggg
     3061 gctctactgt cgatctctct ttcgatttgg agtcccaaat catcccggta aaaggaaacc
     3121 attttatcta ggtccctgac tacaaaccct gaatgattga atcctgtaat catactttcc
     3181 tcctggtagg tgagttgtaa tttgagtatg cgcttatcac attgcaaacg atataatcgc
     3241 agtgtattct tcagagtatg aatgaactcc ttaatatact caaactgcct tatctgtggg
     3301 gcggcatagg tgcagctctt ggtgcggggt tgggcgttaa caatttatca gtttggctgc
     3361 tggctgtgtt gttgggatta ttttttgtaa ccatgagact caccggtccg ccagaagaag
     3421 gtaaagaagg tagattgttc gcagggggga gtttgcttat gcttggttgg attttggctt
     3481 tttcaatacg tgggatagtt atttaattcc taagctgcat agaaactttg agaatatacg
     3541 ctttttaaca agttaaaggg aacaaaaatt cgtgtctacc aaagtttcct tatttgcaac
     3601 atgtattctt aataatttct atccggatgt tgcatttagt gtggttaggg ttttgagtaa
     3661 attgggagta gatgttacgg ttcggccgga tcaaacctgc tgcggacagc cgtttttcaa
     3721 ttccggacat tggaacgatt catccaagtt gatgagaaaa ttcgttgaga gctattccgg
     3781 tggccaagaa gatatagtgc taccgtcagg gtcatgtact agcatggtaa gaaatcatta
     3841 tcttgaagta tgcaatgatg atgacattgg tgagataaga gacgtgtcag gcaggacttt
     3901 tgaattcaca gaatatataa ctagagaact aggtatattt gatctgaccc cgttccaatc
     3961 cgccataact gaaaaaatca atgtgaccta tcatgaatct tgccacctca aaagggaatt
     4021 gggtgtttcc agtgaaccga ttgatcttct acagtcactc gaaaatgtga cagtaatgga
     4081 tatggaccaa tctgaagttt gttgcggttt tggaggtacc ttcgctacaa aatattcgga
     4141 aatatccacg gctatgggag aagaaaaagt cgctaatata gtgaaaagtg gcgcagatat
     4201 tgttaccgca tcagacatga gttgtttgat gcatattgac gggttgattg cacgaaaaaa
     4261 aattcccctc aaaagcatgc atatttcgca agtaatagag caatctttga atatggaata
     4321 tcaaaaatga aaccagagac gaaaaatttc aattcgaaat ccaaagagac cttattggat
     4381 cctcagattc agaaaaattt atccgggctt tatggagggt tccatagtgc gcgtatcaaa
     4441 gcatcctcag atacggtcga ttgggaagaa ttacaaataa aaggtaggga aatcaaaaag
     4501 cacgtaataa ataatttgga ttactatttg gagttattgg aatctaacgt gatttcctcc
     4561 ggtggtaatg tttattttgc tgagacagct gaagatgcga ctcagtacgt tataaattta
     4621 gcgaaggaga aagacgttaa gacggttgtt aaaagtaaat ctatgttgtc agaggaaatg
     4681 tcgctcaacg aggaattgat caagcatgga atagatccat atgagactga cttgggcgaa
     4741 tacattgtac agctggcaga agagactcct taccatatta ttgctccagc aatacataaa
     4801 tccaggcaag aagtgtctga attgttcgct gataaaattg gtacaagcca gacagaaaac
     4861 atagaaactt taaccttaga ggcacgagac caattaagag ataaatttat tcaagctgat
     4921 atgggaataa ccgggggcaa ttttttggta gccgaaactg gcacattggc cttggtatcg
     4981 aacgaaggta acggtcggat gtgtacttca atgcctcctg tacatgtcgc tattgttggc
     5041 atggaaaaaa ttattccatc tatggaagat ttgggca
//
LOCUS       DASCNS010000169        17991 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001313073, whole genome shotgun sequence.
ACCESSION   DASCNS010000169 DASCNS010000000
VERSION     DASCNS010000169.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17991)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17991
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001313073"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="QF838_06065"
     CDS             <1..640
                     /locus_tag="QF838_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60279.1"
                     /translation="ETKWLQEVYQNTGFPNAIVGHVNLRGSEAGHVIERHLNHNNFKG
                     VRDFSYGEYLVSKQFRRGFALLEQYSLVSSIAAQWQDMEKLADLAQSYPNTTIVLDHA
                     GFPSKRGIEYFTNWKVGMEKVSNFDNIFCKISGLGMGDNNWTTESIRPYVETCIELFG
                     STRSLFATNWPIDSLWSEYGDVVKAYREITSNYSETEIGRLFSKNAEEIYQL"
     gene            complement(999..1529)
                     /locus_tag="QF838_06070"
     CDS             complement(999..1529)
                     /locus_tag="QF838_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60280.1"
                     /translation="MKEGIQTKNRIYEFDIVIFAIGFDAMTGALLGMNITGKNGRNLK
                     EIWSEGPKSYLGLSMSGFPNMFTITGPGSPSVLSNMMTAIEQHVEWIRDCINYMEQGG
                     YSEIEAELPAEEYWTNHVEEIAGNTLRYTCNSWYVGANIPGKKRIFMPYAGGIPPYRE
                     YCDEVAKNSYDGFKIL"
     gene            complement(1671..2063)
                     /locus_tag="QF838_06075"
     CDS             complement(1671..2063)
                     /locus_tag="QF838_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60281.1"
                     /translation="MVIIRTGSSAVQAIPVIAKEAKHLTVFQRTAKYSIPAKNRPLIE
                     REVEVVKSTYSEIRAEAKANRAGIASFKAGFDSALSVSEIARTQEYQKRWDEGGTGFT
                     AAYSDIGTSDEANFTAAEFVRKKIPETV"
     gene            complement(2115..2630)
                     /locus_tag="QF838_06080"
     CDS             complement(2115..2630)
                     /locus_tag="QF838_06080"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJO60282.1"
                     /translation="MTSLKSESDHINSGAIVYDVVIVGAGFAGMYMLRELRETGLNVL
                     VLEEATGVGGTWYWNRYPGARCDVESMEYSYSFSEELQQEWNWSHRFSTQPEILEYAN
                     HVADRFDLRRHIKFQTRVTSVKYDQDNQKWLLKSSSKEEFQGRFCVMATGTLSSVNEP
                     KFKGIKDYQGD"
     gene            complement(2706..3191)
                     /locus_tag="QF838_06085"
     CDS             complement(2706..3191)
                     /locus_tag="QF838_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019846.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJO60283.1"
                     /translation="MLDFTLLDLMSSWRSHIPDKLTTHEVYGLGLNREEMSDNPQLDY
                     WVTKDINKDANFPYEDNKFDAAMVIASIQYMTDPISVFNEVNRVLKNNGKFHVVYSNR
                     MFPTKATKIWKVFDNIERASLIGSYFGDSGSWSVPKAVDLSPPGDVVSDPLFVVSAQK
                     V"
     gene            complement(3790..4116)
                     /locus_tag="QF838_06090"
     CDS             complement(3790..4116)
                     /locus_tag="QF838_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HJO60284.1"
                     /translation="MAKYLITGSYTAEAWAAQIADPKNRIEVVRPAFEKMGGSIESAY
                     LSFGAGDLVIIADMPDNVSAAALSMAVSAGGGVANLQTTPLISVEEAVTAMRKAAEIT
                     YAPPGN"
     gene            complement(4302..5087)
                     /locus_tag="QF838_06095"
     CDS             complement(4302..5087)
                     /locus_tag="QF838_06095"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO60285.1"
                     /translation="MSTNLGRLKGKVAIVTGSGSRGDVVGNGQATAILFAKEGAKVML
                     VDSNGENLIRTQQKIESTKGECITYVGDVTKSATCQEVVEKTIRVFGRLDILHNNVGI
                     GGSGTVVEVSEEEWDKVMQVNIKSMMLMGKYSVPEMVMRGGGSIINISSISAIRPRGL
                     TPYTVSKSGVIALTKAMAVDHAEDGVRVNCILPGPIYSSMTAPNMTPEKRQTRQNASP
                     LKQEGTPWDVGWAAVYLASDESKWVTGVMLNVDGGVTLGSPPR"
     gene            5134..5646
                     /locus_tag="QF838_06100"
     CDS             5134..5646
                     /locus_tag="QF838_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60286.1"
                     /translation="MTLEDFISRFNSTELQKALNIQHLSDIGTTDDKIIKLAAACLTK
                     SEIAYLLGGLEEYRLREISLEFEVASAATSSRKRLTNRVIQIVLDEYEPFGQSLTKIK
                     NLKTLILLSAAALMTMIIFITTALLYSNAIAFLSAIAFAIPASLYLYGSIKNRLQKKR
                     SKSELIEPVR"
     gene            complement(5626..6702)
                     /locus_tag="QF838_06105"
     CDS             complement(5626..6702)
                     /locus_tag="QF838_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HJO60287.1"
                     /translation="MLERFHVPEDIAVRVQQEDMRAAVEDIFVKMRMPARDAALAADV
                     LMYADIRGVESHGVSNMMKSYVAGFQEGRINPTPDWKIVREALAVCTIDSDQGHGLIV
                     GPAAMNMAIERAEKYGIGSVSVNNGAHFGAAGYHAMMALEHNMIGIAMTTGGVSVLPT
                     NGSERLVGLNPLAIAAPTRLEAPFIFDASMSSVAGNKITLAKRLGVDALPGWVADSDG
                     SPIMDERQVPEDFKILPLGGTRELGSHKGYSLAVMIEILSAVLSGGGAGPNRKAGASH
                     HFLAYKIDAFVDVDMFKDDLDQYMKTLRESAPAPGHDNVTYAGLPEHEEEKERLENGI
                     PYHPEVIEWFTDIAAELQLPNRFN"
     gene            7011..8534
                     /locus_tag="QF838_06110"
     CDS             7011..8534
                     /locus_tag="QF838_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HJO60288.1"
                     /translation="MGKHKNLVFIFSDQQRYDTMACYGNDWIKTPNLNSLSEESFVME
                     RAYVGQPVCTPARGTITTGLHPHNAGPTVNVIPLPAEQKTIAEYLPSEYETAWFGKWH
                     LGDDGNAQHGFKHWISTEDGVSRFVGLSTPPSKSDWHNYLVEKGYSPDSTLHSDRPHL
                     PEEVTQLAPDNWEVFSSEKRSELPPEDQMAHFLGRNAVEFIEHHKDSPFCLYVSTFEP
                     HSPYNGPYNDLYDPASLPVGPAFLKKPENAPLISRLRAENNMQFLEGSSGSYRGYVEK
                     NIVGSHNDISSEYGWRELRARYLANITLVDDMVGMIVKSLEEQGVLDDTVIVFTSEHG
                     EMAGDHGMLEKRSMYEESARVPMVIRAPKIGTDKMVVSGNFSHVDLVPTLLDLLGVDV
                     PDGLDGKSVAEVLEDGSNLRNNEVFVEWNGIGSIEDRLLGTPDINSMHQAPWRTIVYR
                     DWKLNLCATDKCELYNLREDPNELNNLIDSPDKQDVVRLLSTKIRAWQYKTNDKAPL"
     gene            8586..10394
                     /locus_tag="QF838_06115"
     CDS             8586..10394
                     /locus_tag="QF838_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60289.1"
                     /translation="MKLRVGTLVSFGVVAVMVMALVGCSSSDSGAAPAAPAAAAPAQA
                     GSSSGYVPAAPAQPAAAAAAAAAAAPSAEVETAKKVSAKPKQTVAKTVTEFEGGKRGG
                     VMKINTQKWFQRWDMTTRSHWSSTQGLNRHYSGVLQFSPRDGLTVTPDLAKEWTLTDD
                     LMGSTFVFNEGVKWHDGEDFSVDDVIYTINRWQNPPEGVAQPRVAGLLLVDSMEKVGD
                     NTLKITHTKPTSRILPAMADAWHIIHPEHVLSETGGVLSSGEQVIGTGPFKVDSFSEG
                     NNIVSVANPDYFQDAPDGSPFPYLDGITGIAFANNDLSAAALATGQIDYYTSLTPSKS
                     FPIKNKRPSIIITRWDSPGFQGISMNTTKAPFDDLELRKAFACGLNTHAIAELSSTPG
                     VVPMEFRQISFFGTADPNFENVAGLKCVDPAQREANEAESMAIFKAKGLTEVEFDSDN
                     PDDPIATIVQQQMKELGVDVKIVTSEVTSAREKAYAGDTTLFFFGQAVASKSPVEIIN
                     QVYRPGAGFELYQATPPAKWSELLDQLDMATLGSEEEKALTAQMDTIMWEEWMPKVPV
                     RRPDEFQVKQDYVRNIDAIATAKFIQSRFVDAWLDR"
     gene            10555..11508
                     /locus_tag="QF838_06120"
     CDS             10555..11508
                     /locus_tag="QF838_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60290.1"
                     /translation="MRQYILRRLLLLPPTLILLGTILFFMVQSIPGDVASALLAGEEN
                     AVDPKTLEKFREDLGLNEPLIVQYGKWLVQTATGDLGYSYYFSKPVWEVIKPKIETTV
                     TLAIFGVVIAIVLALPAGIFSALFRGSWFDQSVRTISAAGMAVPAFWLGIIILLVLSR
                     IVGWMPPVIHSSIFDNPIDAFQRYLFPSLILGFRSAAVISRMVRSMMLEVLSEDYVRT
                     AWAKGLQPRSVILGHALRNALLPVVTMLGMLFATLIDGAVVLETVFNLPGIGLLMVDS
                     VIARDPGMVLGLVLSIGIFMMIWILLIDLSYKVLDPRVEYD"
     gene            11535..12425
                     /locus_tag="QF838_06125"
     CDS             11535..12425
                     /locus_tag="QF838_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60291.1"
                     /translation="MNLDTETSKRSEIARYSWRLIRQNPAGAIGAFVVFVVLVGAVFA
                     PFITPYDYESFASSRYTKPMGNATNGGLMLLGSDNLGRDQFTRLLYGGRISLLVGFVS
                     PIVGVAIGSLLGIISAYYGKWTDLLLQRITDTLLIIPGLVILMTVTASFGFTITVVLL
                     ALALFSMIGSIRVVRSHVLGLRGAQYIDAQRAIGSSDLRVIFIHLLPNTLPVAVVLIA
                     VGIAGAIVAEAQLSFLGLGIAPPVPTWGNMLNGAQARFDLGPHLAIWPGIFISVTVLG
                     LNLVGDAVRDIMDPRLRGRQ"
     gene            12471..14033
                     /locus_tag="QF838_06130"
     CDS             12471..14033
                     /locus_tag="QF838_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HJO60292.1"
                     /translation="MSDRKPNIVFMFSDQQRYDTMAAYGADYMQVPHLNQLSEESFIF
                     QNAYVAQPVCTPARATIMSGLYPHTAGPIVNMINLPAEVKVISEMISDEYYKGYMGKW
                     HLGNDIERQHGFNVWKSSEDSHGTGYTHTQLNNETSSYTEYLIEKGHEPDNVPAIHGE
                     NVAAGRKFNLLGRKSGQRAPTFTSNAHYDMPEEDQMASYLAQEATKFIEDNKDKPFVL
                     YVSTFEPHSPYHGPFMDQYDPTTLPVGPAFLEKPESASLVNRVRADYFMKFMLDGEDQ
                     TQDAYMMNYAAAREDVTTEAGWRQLRAHYMANITLVDNMVGTINQAIKDAGIEDNTIV
                     VFTSEHGDMMGDHGMLEKRSMFEESARVPLLMKVPWLSKGAQVIEGSVGHIDLVPTLL
                     DLIGQEVPNHLQGKSLKPVLEGESDLSENDVFISWNGYDNNIPDRFLGDIKINRMLRL
                     PWRTVVTPDRWKLALCAGDQCELYDLNDDPYEQNNLFNDPDQQDRVRDMAARIRVWQV
                     QTGDTAPIPSTI"
     gene            14147..15658
                     /locus_tag="QF838_06135"
     CDS             14147..15658
                     /locus_tag="QF838_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HJO60293.1"
                     /translation="MAKQPNLVFVFSDRQRFDTLEAYGNDYVKAPNLNALSKESIVFK
                     NTYVTQAVCAPARASIMTGLYPHAAGMPRNKLVMPEEVETIANMVSADYKKAYFGKWH
                     LGNEVINQRGFDEWASGMETLRDEYTEEEYLDMDTSYHEFLVENGFEPDELLLGSKIF
                     SDEMRAALPAEFQMASFFANRAETFIKENKDNPFVMYVSFLEPHPPWTGPYDGVHDPQ
                     QVPIEPTFLKRPDGHSLFSRVRADFFMQSDLHTPDDVDDSEWEKGREEYNALDKAIGR
                     QFDLKNPDSWKQIRANYYGNITLVDDAVGKILNALEEAEIAENTIFIFTSEHGDLVGT
                     HGMMEMRTFYEEAAKVPLIIRAPMHTKEAKLIDGNFGQIDLVPTLLDLLGQDIPEHLQ
                     GTSKSDVIKGKADLKGNEVFMEHNGIGDRNLGTPAINMLNNMQWRSIVTGDRWKLNLC
                     ATDQCELFNLNEDRFEENNLFNNHEHKDRIRIMAAKIRMWQYETGDHAALPDV"
     gene            15957..16880
                     /locus_tag="QF838_06140"
     CDS             15957..16880
                     /locus_tag="QF838_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60294.1"
                     /translation="MGPLNLHAAIANYGHTTTLKDGTFTSDEFELTHTEISPVPMIFR
                     RMVRNLEFDIAEMALATYICSKHYKKGFTALPIFLTRAFYHGGIMCNANSGIKKPSDL
                     NGKKIGTRSYTLTPGIWTKAILQTEHNVDLDSVTWVLSGDEHVAEYQTPSGVISSEHN
                     DLSKMLLSGEVDAVIGAGTIDSPDAVPLFEDPDELDTGWYNKTKIYPISHLLVVRDDL
                     LLAEPWLEAEIYALFKEAKDSYVKSLPSLSKIDANDKQNKKMAAIVNGDPLPYDLNGA
                     YQGINTFIKFNVDQKIIPNYVNPESLFTMPK"
     gene            17081..17929
                     /locus_tag="QF838_06145"
     CDS             17081..17929
                     /locus_tag="QF838_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60295.1"
                     /translation="MKQLMLVIAISILSVAALACGPAVGRAVGDAQVDVDATLAEMGE
                     PTTEAEKELYAMMKEIDRLHKEEREDLLGALDDSGNIINNLVGVIDSLGNELDEETLI
                     KIGSVVASGQDVDAFFQGVREQAYQNSAKEDEINKLLQNEERATWDRIKFLNEQLDKA
                     NAEKAGIKKDLDKAHVNLDKADKDEDKWIKSNLDKEQEKQILEVRMERAAGKFEVVTA
                     MIDQVLGDDDDDVADAPSAFEKSAEEAGRYQAAGIFHNCLVNGGVEVKETTREEMKHC
                     RQGVSE"
BASE COUNT         5405 a         3896 c         3797 g         4893 t
ORIGIN      
        1 agaaactaaa tggttgcaag aagtttatca aaacacaggg ttcccaaacg ccatagttgg
       61 acatgtaaac ctaagaggta gtgaagcagg ccatgtaata gaacgtcatc taaaccacaa
      121 taatttcaaa ggtgtcagag atttctcata cggggaatac ttagtgagca agcaattcag
      181 gagaggattc gcactacttg aacagtacag ccttgtttca agtatagcgg ctcagtggca
      241 ggatatggaa aaactcgctg atttagccca aagctatcct aataccacca tagtacttga
      301 tcatgccggg tttccttcga aacgaggtat tgaatatttc accaattgga aagtcggaat
      361 ggaaaaggtt tcaaattttg acaatatttt ttgcaaaata tcaggcctcg gtatgggaga
      421 caataactgg actacagagt ctattagacc gtatgttgag acatgcatcg aacttttcgg
      481 atcaacccga tctttattcg ccacgaattg gcctatagac agcctatgga gtgaatatgg
      541 tgacgtggtt aaggcctatc gtgaaataac tagtaactac tcagaaacgg aaattggaag
      601 attgttttca aaaaatgcag aggagatata ccaactgtag cgcaaccgct ctgtcagtac
      661 gcggaggtcg gtgcacaata aatttttatc atggcagccc aaccgacaac aggtattagc
      721 cataaaaaca acaaccacca tcttttattt tgatcatgga ggcgccgtac acttattgag
      781 aaagtgggac atagcatcac acaccaaaag atcgcagtca gaggataaaa aggtaggaaa
      841 ctaaataaaa ctgcgtcgat gatgattaag ggcaagctgc aaatcatttc aaataagagg
      901 aagaaccata tttcacctct ggtagaccgg ccttcaaaat ctgaccatct cctaaagccc
      961 aaatttattg caagtgagaa tgtcaaattt ctttgtccct ataggatttt aaatccatca
     1021 tagctatttt ttgctacctc atcacagtac tctcggtaag gtggaatgcc cccagcatac
     1081 ggcatgaata ttcgtttctt gccaggtata ttggctccga cgtaccatga attgcaagtg
     1141 tatcgcaaag tattcccggc aatttcctcc acgtgattcg tccaatattc ttcagcaggt
     1201 aactctgcct ctatttccga gtagccacct tgttccatat aatttatgca gtctcttatc
     1261 cattccacat gttgttcaat tgcagtcatc atgttggaga gtacagaagg gctacctgga
     1321 cccgttatag taaacatatt tggaaatcct gacatactta atccaagata actttttggc
     1381 ccttccgacc atatttcttt aagattgcgt ccattttttc cagttatatt catacctaat
     1441 agagctccag tcatggcatc aaacccgata gcgaaaataa cgatgtcgaa ttcataaatc
     1501 cgattttttg tttgtatccc ttctttcact aaaccttgga ttgggtcaga attgacatca
     1561 aggagagtta cgttactgcg gttataagtt tcataataat cagtatcggc acacagacgt
     1621 ttacaaccaa tagtattagt cggggcaagt agttctgctg tttctgaatt ttaaactgtt
     1681 tccggtattt tctttctaac aaattcagct gccgtgaaat tagcctcatc gctggtccca
     1741 atatcagaat aggcagccgt aaaacccgtg cctccttcat cccatctctt ctgatattcc
     1801 tgggttctag ctatttccga aactgataga gctgaatcga acccagcctt gaaagacgct
     1861 attccagcac gatttgcctt tgcctcggcc cttatttcag aatacgtact ttttacaact
     1921 tcaacttccc tttcaatcaa aggcctgttt tttgcaggaa tggaatattt cgccgtcctt
     1981 tggaaaacgg ttaaatgctt ggcttccttg gctatcaccg gtattgcttg cactgcagag
     2041 gaaccagttc ttatgataac cacagtttta cccgaaaaat cgactctatt atgaggccat
     2101 ctaccagtta catatcagtc accttgatag tctttaatgc ctttaaattt tggttcattc
     2161 acggatgaaa gagtacctgt agccatcaca caaaacctgc cctgaaattc ttccttagaa
     2221 gaggatttca gtaaccattt ttggttgtct tgatcgtatt taacggaggt caccctcgtt
     2281 tggaatttga tatgccttcg caaatcaaac ctatctgcta catggttagc atattctaat
     2341 atttcaggct gggtggaaaa cctatggctc caattccatt cttgttgtaa ttcttcagaa
     2401 aaagaatagg aatactccat actttccaca tcacatctag cacctgggta cctgttccag
     2461 taccaggtgc cgccgactcc tgttgcctct tccaaaacaa gtacatttag acctgtttcc
     2521 cttagctcac gcagcatgta catgccagca aatccagcac ctacaattac cacatcatag
     2581 acaattgctc cactatttat atggtccgat tcagatttaa gtgaagtcaa ttttgaccac
     2641 cgtaagaagt ttatttaaca aaattatatc caattctctg gtagtagtct ttagcctcgc
     2701 attggttaca ctttttgggc agaaacgaca aataaaggat ctgacacaac gtcgccggga
     2761 ggactcagat ctactgcttt agggacactc cacgaaccag agtctccgaa ataactccca
     2821 atcaaacttg ctctttcaat attatcaaat accttccata tttttgtggc ctttgtggga
     2881 aacatacggt ttgaatacac cacgtgaaat ttaccattgt ttttcaaaac cctgttgact
     2941 tcattgaaaa cagaaatcgg gtccgtcatg tattggatgg atgcaataac catagccgca
     3001 tcgaatttat tatcttcata gggaaaattc gcatccttgt ttatatcctt ggtaacccaa
     3061 taatcaagtt gtggattatc ggacatttct tctcgattaa gtcccaatcc atatacttca
     3121 tgcgtagtga gtttatcagg aatgtgagag cgccaactac tcattaaatc aagcaacgtg
     3181 aaatcaagca acgtgcaatt ttcaggcaat tgagcaaaaa ggtatttcta acactgaacg
     3241 atggcagctt catcaatatg aactaccaac ctggcctcgg aataaaacaa agcatcgtcc
     3301 gattcatctt ctcgtttaaa tgaattttct ggatactcca tgtatcaaac ctttaattta
     3361 cggggtagcc ttcataagac ttttcctatc aaatatttcc catcattcca ttctggttca
     3421 gaagccagat attgcatcat attagtgaag ttttcatgtt gttccggttt catgttgttc
     3481 cggtctgttg gcgtttttaa gataagcatc tttactttca aacactgcta ccccaactag
     3541 atctgttgtc attttatccg gtttcattaa aaaaaagcca cagcccccag agggttttct
     3601 aatttgtcga aactagctat caattcctgt tcacaacctt cttttacgtc taagtcaaat
     3661 atagttccgt acatagaaac actctccata tcctgtcggc ctagtcgacc ttaaatttat
     3721 atttgcaaca aaaaataccc cctacatcca aaaatataag gggtattaaa atttccttgt
     3781 attagctact taattcccag gaggagcgta ggttatttca gctgcctttc tcatagcagt
     3841 tacagcttcc tcaacagata tcaatggagt cgtctgcaag tttgctacac cgccaccggc
     3901 tgaaactgcc atcgatagcg cagcagcact gacgttatct ggcatatcgg cgataatcac
     3961 taagtcacct gcaccaaatg aaagataagc agactcaatg gaccctccca ttttttcaaa
     4021 cgcaggtcgg acgacctcaa tcctgttttt cggatcagct atctgagctg cccacgcttc
     4081 agctgtgtat gagcctgtta ttaaatattt tgccattttc tatgctccta gtgtaatttc
     4141 tatgcctaaa cttaccaaaa atgcatcctc cgctcgaatt gctggctaac gatgtcataa
     4201 ttggaacatc accccagcaa gcgttttcat attagcattt tttaggttga catactcgaa
     4261 aactatttat tacggtaaat ttcaacctag ctaaaaccga cttaccttgg agggcttcca
     4321 agagtcacgc ccccgtctac gttgagcata acacccgtaa cccacttgga ctcatcacta
     4381 gccaaataaa cagcagccca ccctacatcc caaggagttc cttcctgttt taagggagag
     4441 gcgttttggc gagtctgtct tttttctggt gtcatgtttg gagccgtcat tgacgaatat
     4501 attggcccag gcaaaataca attgaccctg acaccatcct ccgcgtgatc aaccgccatt
     4561 gctttggtaa gagcaatcac tccacttttt gaaactgtgt agggagtcaa ccctctaggt
     4621 ctaatcgctg atattgacga aatattaata atggacccac ctcctctcat caccatttca
     4681 ggcacgctat atttacccat taacatcata gatttaatat tgacctgcat gactttatcc
     4741 cactcctctt ctgatacctc aacaacagtc ccagaacctc cgatcccaac attgttatga
     4801 aggatatcta acctcccaaa tacccttatg gttttttcga ccacctcttg gcaagtcgct
     4861 gatttggtca catcgccgac ataggttata cattcgcctt tggtggattc gattttttgt
     4921 tgggttctaa ttaggttttc tccgttagaa tcaaccaaca ttaccttggc tccttcctta
     4981 gcaaataaaa ttgctgttgc ttgaccattt cctacaacat cgccacgtga gcctgaacca
     5041 gtgacaatgg ccacttttcc ctttaatcta cctaaattcg ttgacataaa gtaacctgcc
     5101 ttttcgagct aacggtgcaa aatttgtgca attatgacat tagaagattt tatttctaga
     5161 ttcaactcca ctgaattgca gaaagcttta aatatccaac atctttccga catcggaact
     5221 actgatgaca aaatcatcaa actggcagcc gcatgcctta cgaaatctga aatcgcttac
     5281 ttattgggag gactggaaga gtacaggcta agagaaattt ctttggaatt tgaagtagca
     5341 agtgcagcca catcaagtcg aaagcgacta actaataggg ttattcagat agtgttagat
     5401 gagtacgaac ctttcggcca aagtctcact aaaattaaaa atctcaaaac actgatcctt
     5461 ttatctgcag ccgctttaat gactatgatt attttcatca ccacagctct tctttacagc
     5521 aatgcaatcg cattcctttc tgcaattgca ttcgccatac ctgcaagttt gtatctctat
     5581 ggaagcataa aaaacagatt gcaaaaaaaa cggtcaaaaa gcgagttaat tgaacctgtt
     5641 aggtaattgt aattctgctg caatatcagt gaaccactca atcacctcag gatggtaagg
     5701 tataccgttt tccagtcttt ccttctcttc ttcatgctca ggtagacctg catatgtgac
     5761 gttatcatgc cctggagctg gtgcagattc tctgagagtt ttcatatatt gatccaaatc
     5821 atctttaaac atatccacat cgacaaatgc gtcaatcttg taagcaagaa aatggtgaga
     5881 tgccccggct tttctatttg gtccggcacc accaccagat aaaactgctg aaagtatttc
     5941 tatcatcaca gcaaggctgt atcccttatg ggatcccaat tcacgtgtcc cacccagtgg
     6001 gagtatctta aagtcctccg gcacttgtct ttcatccatt attggtgaac cgtcggaatc
     6061 cgcaacccat ccaggaagtg catccactcc taatctttta gcgagggtga ttttattacc
     6121 tgcaacggaa ctcattgaag catcaaaaat aaagggggcc tccaaccggg taggggctgc
     6181 aattgccaaa gggtttaatc cgactaacct ctcagaccca ttggtaggca atactgaaac
     6241 cccaccagta gtcattgcta ttccaatcat attgtgttcg agtgccatca tggcgtgata
     6301 tccagccgcc ccaaagtgag cgccattgtt cacagacaca gagccaatac catatttctc
     6361 tgccctttcg attgccatat tcatggcagc cggccctact ataagaccat ggccttgatc
     6421 actatcgatt gtacaaaccg ctaaagcttc cctaactatc ttccagtctg gagtaggatt
     6481 gatacggcct tcctggaaac ctgcgacata actcttcatc atgttgctta ccccatgcga
     6541 ctccacacct cttatgtctg cgtacatcaa cacatctgct gctagagcag catctctagc
     6601 tggcatcctc atttttacaa aaatatcttc tacagcagcc ctcatatctt cttgttgcac
     6661 tcgtacagct atatcttccg gtacatgaaa tctttcaagc acagtaaact cctgatttta
     6721 tggattagta agcagcccct cccctataaa tataatctcg aacagtacta cgattttttg
     6781 taaatagcac actttttaat aagttcccaa tcgtgaaata tttactatgt aaacaccttt
     6841 cactaaagaa cccggtatca actcattcta ttttctatat caaaaaatgt aattattatt
     6901 tcttgataaa ttagcctata tcgttgacat cacaagaaag gaagctatac cctcatttcc
     6961 tgccttttaa tagttgtgtt acctaacaaa accggagata gaaattcact atgggcaaac
     7021 ataaaaacct agtttttatt ttcagtgatc agcagcgtta cgacactatg gcttgttacg
     7081 gtaatgactg gataaaaact ccaaacctaa attcacttag tgaagaatcc tttgttatgg
     7141 aacgagccta tgtggggcag cctgtttgta cccctgccag aggaacaata accacaggcc
     7201 tccatcctca taacgcagga cctactgtaa acgtaatacc tcttccggca gagcagaaaa
     7261 ctatcgccga atatttacca tctgaatatg aaacagcatg gtttggcaaa tggcatttgg
     7321 gggatgacgg gaatgcccaa cacggtttca agcattggat ttcaacagaa gatggtgtgt
     7381 ctcggttcgt ggggctcagt acaccgccaa gcaaaagcga ttggcacaac tacctggtag
     7441 aaaaaggtta ttccccagat tccacgctac atagcgaccg acctcatttg ccagaggagg
     7501 taacacaact tgccccggac aattgggagg tattcagcag tgagaaaaga tctgaacttc
     7561 ccccagaaga ccaaatggct catttccttg gaagaaatgc agttgagttt attgaacatc
     7621 acaaagacag ccctttctgt ttgtatgtga gtacatttga accacactcc ccctacaacg
     7681 gcccttataa tgatttatat gaccccgctt cactgcctgt gggccctgca tttttgaaaa
     7741 aacctgaaaa tgcgccgctc ataagcaggc ttagagcaga gaacaacatg caattcctcg
     7801 agggctcttc tggttcatat agagggtatg ttgaaaaaaa tattgttggt agtcataacg
     7861 acataagttc cgaatatggg tggagggaat taagagctag gtacctcgcc aacataactt
     7921 tagtagacga tatggttgga atgattgtta aatctctcga agaacaaggc gttcttgatg
     7981 atacagtgat tgttttcacc agtgagcacg gagagatggc tggagatcat gggatgctcg
     8041 aaaaacgttc catgtacgaa gaatccgcaa gggtgccaat ggtcataagg gccccaaaga
     8101 taggcactga caagatggta gtcagcggta actttagtca tgttgacctg gtccccacgc
     8161 ttttggatct attaggtgta gatgtccctg atggactaga cggtaaatca gttgcagaag
     8221 tcttagaaga tgggtcaaat ctccgaaaca atgaagtgtt cgtagaatgg aatgggatag
     8281 gatcgataga agatcgtcta cttggcactc ctgacattaa ttctatgcat caggcaccgt
     8341 ggaggacgat agtttacagg gactggaaac tgaatctatg cgcgactgac aaatgtgagc
     8401 tctacaactt gagagaagac cctaatgaac tgaataattt aattgatagt cctgacaagc
     8461 aagatgtagt gagactgcta agcacaaaaa taagagcgtg gcaatacaaa actaatgata
     8521 aagctccgct ctagcttcaa ggaaatgata ttaacaccta aaggaccttt caaagggagg
     8581 attcaatgaa attaagagtt ggcacactag taagctttgg tgtcgtagcc gtaatggtca
     8641 tggcactagt aggatgtagt tcctctgatt cgggtgcagc acctgcagca ccagcagcag
     8701 cagctcctgc acaagcaggt agcagttcag gatatgtccc agcagctccc gcccagccag
     8761 cggcagccgc ggcagcggca gcggcagcag ctccctctgc tgaagtagaa accgccaaaa
     8821 aggtctctgc taaacccaaa caaaccgtcg ctaaaactgt caccgagttc gaaggtggta
     8881 agcgtggtgg tgtgatgaag attaacactc agaaatggtt ccagagatgg gatatgacaa
     8941 cccgatctca ctggtcatcg acccagggac tcaaccgcca ctacagcggt gtacttcagt
     9001 tttctcctcg agatggacta actgtaactc ctgacttggc taaagagtgg actttgactg
     9061 atgatcttat gggatcgacg tttgttttta atgaaggtgt caaatggcac gatggtgaag
     9121 acttcagcgt ggacgatgtc atatacacca ttaatagatg gcaaaaccca cctgaaggtg
     9181 tagcacaacc tagagtagca ggcctacttc ttgtagatag tatggaaaaa gttggtgaca
     9241 atacactcaa aataactcac acaaagccaa caagtcggat acttcccgct atggctgacg
     9301 catggcacat aatccatcca gaacatgtac tttcggaaac cggaggggtt ttaagttctg
     9361 gagagcaagt tatcgggaca ggaccgttca aagtagacag tttttctgaa ggaaacaaca
     9421 tagtgtcagt ggcaaatcct gactacttcc aagacgcacc ggacggtagc ccgttcccat
     9481 acttggatgg aatcactgga atagctttcg ccaataatga tctttcagca gcggcgcttg
     9541 caacaggtca aattgactac tacacatcgt tgacgccttc caaatccttc ccgattaaga
     9601 ataagaggcc atccataatc ataactaggt gggatagccc aggtttccag ggaatttcca
     9661 tgaataccac gaaggcaccg tttgatgact tggaattgag aaaggcgttc gcatgtggac
     9721 ttaacacaca cgcgatcgcg gaattatcca gcacgcctgg tgtcgtaccg atggaatttc
     9781 ggcaaatcag cttctttgga acagcagacc ctaacttcga aaatgttgcg ggtctaaaat
     9841 gcgttgaccc agctcaaaga gaagccaatg aggccgaatc tatggctatt ttcaaagcaa
     9901 agggtttgac agaggttgag ttcgactcag acaatcctga tgatcctatt gccacgattg
     9961 tgcaacagca gatgaaagaa ctaggcgttg atgtgaaaat agtcactagt gaagttacct
    10021 ctgcacgaga gaaagcatat gctggtgaca caaccctgtt cttctttgga caagccgtgg
    10081 ctagcaagag ccctgttgaa ataatcaacc aggtataccg accaggtgct ggttttgaac
    10141 tctaccaagc aacgccacca gccaaatggt cagaacttct agatcaacta gatatggcta
    10201 cgctgggttc tgaggaagaa aaggcactta cagcccaaat ggacacaata atgtgggaag
    10261 agtggatgcc taaagttcct gttagaaggc ctgatgaatt ccaggtcaag caggactacg
    10321 taagaaacat cgatgccatc gcaactgcta aattcattca atctagattt gtggatgctt
    10381 ggctagatag ataatctatc cgaaataaat aaggacctca attccgagac tggtactcct
    10441 taagtggagg aatcagacaa aggattgagg tttcatttaa ctaagtagat actgaggtct
    10501 agacaatcct tggtaatgta acaagcacta acgttccgtc tttaggtaga ggatatgcgt
    10561 caatacatac tcagaaggct gctcctatta cctcctacct taattttgct cggcaccatt
    10621 ttgtttttca tggtgcaatc gatacccggg gatgtcgcat cggctctact tgccggagaa
    10681 gaaaacgcag ttgacccgaa aacattggag aaattcaggg aagatttggg actgaacgaa
    10741 ccacttattg ttcaatatgg aaaatggtta gtccagactg ctacaggaga tttgggatat
    10801 tcctattact tcagtaagcc ggtgtgggaa gtcataaagc cgaaaattga aactacggtt
    10861 actttggcca tatttggagt agtaattgct attgtacttg cattgcccgc tggaatattc
    10921 tctgctcttt tcagggggag ttggtttgac cagtcagtcc gaactatcag cgctgcagga
    10981 atggccgtgc cggctttctg gctagggata ataattctct tagtattatc aagaatagtg
    11041 ggctggatgc cccctgtaat tcattcgagt atctttgata accccataga tgcattccag
    11101 agatatcttt tcccatcgtt gatacttggc tttcgatctg cagcagtgat aagtcgtatg
    11161 gtcaggtcta tgatgctaga agtactgtca gaagattatg tgagaactgc atgggcgaaa
    11221 ggtttgcaac cgagatcagt aatccttggg cacgctctta gaaatgccct gcttcctgtg
    11281 gtcactatgt tagggatgtt atttgccacg ttgattgatg gggcggtagt tcttgaaacg
    11341 gtatttaact tacccggtat tggccttctg atggtggatt cggtcattgc tcgcgatcct
    11401 ggaatggtcc taggattagt cctatccatt ggaattttca tgatgatatg gatactttta
    11461 attgatcttt cctacaaagt attagatccc agagttgagt atgactagag aattgagaca
    11521 ttaaaaaaag caaaatgaat ttagatacag agactagtaa aagatccgag atcgccagat
    11581 actcatggcg gttgatacga caaaacccag ctggagctat cggtgccttt gtcgttttcg
    11641 tcgtcttagt aggtgcagta ttcgctccat tcataacgcc ctacgactac gagtcatttg
    11701 ctagtagccg gtacaccaag ccaatgggta acgcaacgaa tggtggtttg atgttacttg
    11761 gatctgacaa tcttggcagg gatcaattca cccgcctact ttacggtggg agaatctctc
    11821 ttttggtggg attcgtatcg ccaatcgtgg gagtagcgat tgggtcacta ctagggataa
    11881 tcagtgctta ctacggcaaa tggacggact tattacttca gaggattact gacactcttc
    11941 tgataatacc tggcctcgtt atcttaatga ctgtcactgc ttcttttggt ttcacaataa
    12001 ctgtggtgct actcgcttta gctttattca gcatgatcgg cagcataaga gttgttaggt
    12061 cacatgtatt gggattaagg ggcgctcaat atatagacgc gcaaagagcg attggatcat
    12121 cggatctacg agtaatcttt atacatttgc tgccgaacac attaccagta gccgtggttt
    12181 taatagcggt tggaatagcc ggagcgatag ttgccgaggc tcaattaagc tttttaggac
    12241 ttggtatagc tcctccagta ccaacttggg ggaatatgct aaatggtgca caagcccggt
    12301 ttgacctggg accccatctg gcaatatggc ctggaatttt tatatcagta acagtattag
    12361 gactcaacct agtaggggat gctgtaagag acataatgga cccaagacta cgaggcagac
    12421 aataaggcaa atcatttata agaaaattga aaactgttta ggagattgaa atgtcagatc
    12481 gaaaaccaaa tatagtgttc atgtttagcg accagcaacg ctatgacacg atggctgcct
    12541 acggggccga ttatatgcag gtacctcatt tgaaccaact ttcggaggaa agtttcatat
    12601 ttcaaaacgc ctatgtagct caaccagtat gtaccccagc aagagcgaca atcatgtcag
    12661 gattgtatcc acacaccgct ggcccgatag ttaatatgat caacctgcct gcagaggtaa
    12721 aggtgatatc agaaatgata tcggacgaat attacaaggg ttatatgggc aaatggcacc
    12781 ttggaaatga tatcgaaaga cagcacggct tcaatgtttg gaaatcaagt gaagactctc
    12841 atggcacggg ttacactcac actcaattaa acaatgagac gtcctcatat acagaatatt
    12901 taattgaaaa aggacatgaa ccagacaacg tcccagcaat tcatggagaa aatgtcgcag
    12961 ccggcagaaa attcaattta ttgggtagaa aatccggtca aagagcacct acatttacct
    13021 cgaatgctca ttatgacatg cctgaagaag atcaaatggc atcttactta gctcaagagg
    13081 ccacgaaatt tatagaagac aacaaagaca aaccttttgt cctctacgtg agcacattcg
    13141 aaccccactc tccataccat gggccattta tggatcaata cgacccaaca accctcccgg
    13201 taggacctgc ttttctagaa aaacctgaaa gtgcctcatt ggttaacaga gtcagagccg
    13261 actacttcat gaaatttatg cttgacggcg aggaccaaac ccaggacgcg tacatgatga
    13321 actatgctgc ggcgcgggaa gacgttacaa ctgaagcggg ttggcgacaa ttgagggccc
    13381 attacatggc caacattacc cttgtagaca acatggttgg aaccatcaac caagctatta
    13441 aggatgctgg catagaagat aatacaatag tcgtattcac cagtgaacac ggcgatatga
    13501 tgggtgatca tgggatgctt gaaaaacgtt caatgtttga agaatcggct cgtgttccac
    13561 tactcatgaa agtaccgtgg ctttccaaag gagcccaggt catcgaagga agtgtaggcc
    13621 atatagacct tgtgccaact ctgctggacc taattgggca agaagtgcct aatcatttac
    13681 aagggaaaag cttaaaacct gttcttgaag gggaatctga tctttctgag aatgacgtat
    13741 tcatatcttg gaacggctat gacaacaaca taccagatag gttcctcggt gacataaaaa
    13801 ttaataggat gctcaggttg ccatggagga ccgtggtaac gccagataga tggaaattag
    13861 ctctatgtgc tggggatcaa tgtgaacttt acgacctaaa tgatgatcct tatgaacaaa
    13921 ataatttgtt taatgatccc gatcaacagg accgagtgag ggatatggcc gccagaatta
    13981 gagtatggca agtccaaacc ggggatacag cacctattcc ttccacaatt tagttccaca
    14041 acggataaac aggacgagta aggagcattt tatggctttt actcatcctg tttgggcacc
    14101 ccagtattct tacttattac cttaaatttc tttagagagg gacattatgg caaagcaacc
    14161 aaatcttgtt ttcgtattca gcgaccgtca aaggtttgac acattagaag cctatgggaa
    14221 cgattacgta aaagcaccca acttaaatgc actttccaaa gaaagcatcg ttttcaaaaa
    14281 cacctatgta acacaggcgg tctgtgcccc agctagagcg tcaattatga ctggtctata
    14341 ccctcatgcg gcgggcatgc ctagaaacaa actggttatg cctgaagaag tggaaaccat
    14401 tgccaacatg gtctctgccg actacaagaa agcatatttc ggaaaatggc acctgggtaa
    14461 tgaggtaatc aaccagagag gatttgacga atgggctagc ggcatggaga cattacgtga
    14521 tgaatatact gaagaagaat atttggatat ggacacgagt taccacgaat ttcttgtgga
    14581 aaacgggttt gaacctgatg agcttctcct tgggagtaaa atattctcgg atgagatgag
    14641 ggcagctttg ccggcagaat ttcaaatggc ttcatttttt gcaaatagag ctgaaacatt
    14701 cattaaagag aacaaagata atccttttgt aatgtacgtt agtttcttag aacctcatcc
    14761 gccatggaca ggcccatacg atggggtgca cgatcctcaa caggtaccca tcgaaccaac
    14821 tttcctgaag agacctgacg gccattcatt gttcagcagg gtgcgagcag atttttttat
    14881 gcaatcggac cttcatactc ccgatgatgt tgatgattca gaatgggaaa agggcaggga
    14941 agaatataac gcgctagata aggcgatcgg tcgacaattt gatctcaaga acccagattc
    15001 atggaaacaa attagggcta attattacgg gaatattact ttagtggatg atgccgtagg
    15061 gaaaattctt aatgctctag aagaagcaga gatagcagag aatacaatat ttatttttac
    15121 cagtgagcat ggcgatttag taggaactca tggaatgatg gagatgcgaa ccttctatga
    15181 ggaagccgcc aaggtcccgc ttatcatccg ggctccaatg cacactaaag aagcaaaatt
    15241 gattgatggc aattttggac aaatcgattt agtgccgaca ttattggacc tattgggtca
    15301 agacattcca gaacatttac aaggaaccag caaatcagac gtaataaaag ggaaagcaga
    15361 tctaaaaggt aatgaggtgt tcatggaaca caatggcatc ggtgatagaa atctgggcac
    15421 ccctgcaata aatatgctta acaacatgca gtggcgatca attgttacag gcgataggtg
    15481 gaaattaaac ctttgcgcta cagaccaatg cgagctgttt aatcttaatg aagatcgctt
    15541 tgaagaaaat aaccttttta acaaccatga acacaaagat cgaattagga tcatggcggc
    15601 aaagatccgt atgtggcaat acgagactgg agatcatgct gctcttccag acgtataggt
    15661 ggcaggaaac cagggaccga attggctttc tgcagattta gacgattcag gttattggaa
    15721 taattttgaa cggagttttt tgagttttgg cgaaaacgac ttccaatacc cctttcgtat
    15781 aggacgattt ggcattttcc aagctcacag actcagtcca tttcatcatc cacttcaacg
    15841 aaggcttagt tcgctcattc aacaataaat tcctcaatct aggtatattt tataaatctt
    15901 ggtatgatcg ataatcaaaa tgaatatcca ttaaatctca agataaggaa cgaaaattgg
    15961 ggcctcttaa tttacacgca gctatagcta actacggaca taccaccacc ctaaaggatg
    16021 gtacattcac ctctgatgaa ttcgaactta ctcacactga aatttctcct gtaccaatga
    16081 ttttccgaag gatggtgcga aatttagaat ttgatatagc cgaaatggct ctggctacct
    16141 acatctgttc gaagcattat aaaaagggat tcacggctct tccgatattt ctaacaagag
    16201 ctttctacca cgggggaata atgtgtaatg ccaactcggg catcaaaaaa cctagcgatc
    16261 taaatggtaa aaagataggt acaagaagct acactttaac tcctggaata tggacaaagg
    16321 caattttgca aactgaacat aatgttgatt tggattcggt cacatgggtt ctttctggtg
    16381 atgaacatgt agcggaatac cagactccgt ctggtgtgat ttcctctgaa cacaacgatc
    16441 ttagcaaaat gctactctca ggggaggttg atgcggtaat tggggctgga accatcgatt
    16501 ctccagatgc tgttcctttg ttcgaagacc cagacgaact tgataccggc tggtataaca
    16561 agacaaaaat ttaccccatc agccatttgc ttgttgtaag agacgatttg ctactagctg
    16621 aaccttggct agaagccgaa atatatgcgc ttttcaaaga agccaaagat tcttatgtta
    16681 aatcactacc gagtttgtcc aagatagatg cgaacgacaa acaaaacaaa aaaatggccg
    16741 ctatcgtgaa tggagaccct cttccttacg atttaaacgg ggcgtatcaa gggataaaca
    16801 catttataaa atttaacgtg gatcaaaaaa taatcccaaa ttacgtgaac ccagaaagtc
    16861 tgttcactat gccaaaatag ccattaagta ggatttttac aactaaatat ggtcgtataa
    16921 aactaaatat gatcgtataa ttgcagaaac cgaaagatcg aaatccatat aggtgtaatt
    16981 acttaggtgg aattgccgtt gtaatggcta cctatagcct ttattattta aaggacatgt
    17041 gaatcctagt ccaatatgga atcttaatga ggtagagaac atgaagcagt tgatgcttgt
    17101 aattgctatt tccatccttt ctgtcgccgc tctggcttgc ggtcctgctg tgggacgagc
    17161 tgtgggtgac gcacaagttg atgttgacgc aaccctggcc gagatgggag aacccaccac
    17221 agaagctgag aaagagcttt atgccatgat gaaggaaata gatcgccttc acaaagagga
    17281 gcgcgaagac ctccttggcg cacttgatga ttcaggtaat attatcaata acctggtagg
    17341 agttatagac tcacttggga acgaactgga tgaagaaact ttaataaaaa taggttccgt
    17401 cgttgctagc ggacaggatg tggatgcttt cttccaaggg gtcagagaac aagcctacca
    17461 aaactcagcc aaagaagatg agattaataa gctgcttcaa aatgaagaac gtgctacctg
    17521 ggaccgtatc aagttcctaa acgaacaatt agataaagca aacgcagaaa aagctggaat
    17581 aaagaaagac cttgataaag ctcacgttaa tttagacaag gcagacaaag acgaagacaa
    17641 gtggataaaa tctaaccttg acaaagagca agaaaaacaa attctcgaag tcagaatgga
    17701 aagggctgcc ggaaaatttg aagttgtcac tgcaatgata gatcaagttc ttggtgacga
    17761 cgacgacgat gtagctgatg ccccttcagc ttttgagaaa agcgcagaag aagccggtcg
    17821 ataccaagcg gcaggcatat ttcataactg cctagtaaat gggggggttg aggttaaaga
    17881 aaccactaga gaggaaatga aacactgtcg acagggtgtg agtgagtgag tgagtgagtg
    17941 agtgagtgag tgagtgagtg agtgagtgag tgagtgagtg agtgagtgag t
//
LOCUS       DASCNS010000170        13177 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001323283, whole genome shotgun sequence.
ACCESSION   DASCNS010000170 DASCNS010000000
VERSION     DASCNS010000170.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13177)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001323283"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="QF838_06150"
     CDS             complement(<1..675)
                     /locus_tag="QF838_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO60296.1"
                     /translation="MINLENRSSQFALQLKEISKIYPGPPIVQALSDISMSVNHGELL
                     GIIGSSGSGKTTLLHIMGTLSVPSSGSILIDGRNVVGMSDKQLSGVRSQQIGFVFQEF
                     FLLPGFSALENVANGLLYSGIPLDERRERSFEMLGRVALEHRANHLPNELSGGEQQRV
                     AVARALVHNPSFVLADEPTGNLDSENTASLMELFTKLNEDGTTVILITHDLEVAESAR
                     RKITLRD"
     gene            complement(687..2786)
                     /locus_tag="QF838_06155"
     CDS             complement(687..2786)
                     /locus_tag="QF838_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60297.1"
                     /translation="MKKMMRNWKLGLIVAGGILVIAVAVGAFMAKGTYFSQNDQTQAI
                     NSDFNTVLLEQKNLKTYESFSGTLKYEDDVHIITTGEGVLTYLAPEGQGLTRGAEIYR
                     IYQSPEDSELLAADQQVTSADANVAQAELTLENLNSPATAAQIASADANVAQAELTLE
                     NLNSPATAAQIASADANVAQAELTLENLNSPATAAQIASADANVAQAELTLENLNSPA
                     TAAQIASADANVAQAESNLTQGENTITSTDAARLIARQSLCSADKQDEASWLCPNNLR
                     GQVFPDKLISSLIDMISNNILSAEANSLLNAQKNYEAALESLGVLEKNLEQVLSSRLG
                     LDEPSTEAEVLQATQTLNSAKEQRLALNELPTEAELLQANLSLQSSKEQRLALNELPT
                     EAELLQATQSLNSATSSLNIALQNRQDLIEGYSASLLMFGELSAWRDMKEGIAPGEDI
                     NQLKLNLIALGYGTTETLEMDSTFGSNTTAAIMKLQSDLNLISSGEINFGEITFVSGT
                     SMVNFSSTLPNIGGKINTGTELFSLTPIERISTQISSNGAINISSKSLQKVEIQVDVA
                     DQNLVVKGSEVEIELPDESTIVGVVDEVGSFAIVPQDGDPFLEVSIAVEGSAEYFKWT
                     GALVTVNATKELAKGVLASPVNGLLALLSGGYALEIVTETGTILVPVETGIYADGWVE
                     INGSGLQPGTEIVVPSQ"
     gene            complement(2881..3600)
                     /locus_tag="QF838_06160"
     CDS             complement(2881..3600)
                     /locus_tag="QF838_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60298.1"
                     /translation="MKVTIKKDPFTVMAPTLIFCLLCVLLMGCSSKDTSTGVAQLDQN
                     SQKMTAENIPTNTANPLGPDGSLSDLPSPAENGPEQTDEEIATSFAECLRTEGMNVSD
                     PELNADGTVNMMAFRQSLTNDPNFNFQNPRTRQSMQKCVPILQNASFAGQRSQEDEIE
                     LNDNLLEIAQCLRDAGIDAKDPDFSGGRGQVFQSMLGDLSLDRESLQEAMAQCSEAIF
                     GNDASAGAARPPGVRGGPPGR"
     gene            complement(3815..4900)
                     /locus_tag="QF838_06165"
     CDS             complement(3815..4900)
                     /locus_tag="QF838_06165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60299.1"
                     /translation="MKLGCFTMPLHPPGSNITQTMDDDLDQLVYLDKLGYSEAWIGEH
                     FTAQWENIPAPDLFIAKAIAMPEKIILGTGVTCMPNHNPFMIAHRIALLDHLAHGRFQ
                     WGIGSGGFPGDFEVFGFDPSDGTHRNMSKEAVELILQLWEDPKPGRYKSDHWDFTIPE
                     PDDDIGLKFHLSPYQKPHPPIGVASVSPKSETLTMAGERGWIPMSINLVPSNTLAGHW
                     DAVESGATKTKNIANRSDWRIAREIFVAEDGKTAREEALNGILRRDFDDYFHRLMSKL
                     GMLGLYKRDQEMPDEDVTPEYVMENVWIVGDPDEVVAKIRALYEEVGGFGVLLAMGHE
                     WSPLDKWQRSMELLANEVMPKMADLKY"
     gene            complement(5002..6183)
                     /locus_tag="QF838_06170"
     CDS             complement(5002..6183)
                     /locus_tag="QF838_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60300.1"
                     /translation="MRKMTGGSAADAQSILDNVGEIACEFASQRSERQRRRGLQNEDF
                     DRLKESGYPLVAVPTEFGGIWEGDNVGTRKVCEILKTLAQGDSSIALVSAMHPAVIGS
                     AGWSTISTAPEPYTKAWEEQRDWAFQTAREGHFWGTIMSEPGSGGDNTQSTSEAKLAE
                     DGFHYQISGQKHFGSGSGMTSFMVTHAVPEGETSPDTFVLDMRNADWDGSSGVELLAA
                     WDGHGMTSTQSHRMLFENFHATRLAFPGDERRKIIAGMDRPQGAFFVAVITGIVEVAV
                     ATARQQLDSKRHRMRSYEEVEWAKVEIDAWMVQQSYEGMLREVERGSAPARNTLLAKE
                     AVAELAESLLLRISKIIGGGAYSRHAPYGFWLEDVRALGFLRPPWPLAYERVFQGSWD
                     T"
     gene            6447..7070
                     /locus_tag="QF838_06175"
     CDS             6447..7070
                     /locus_tag="QF838_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60301.1"
                     /translation="MDSRKITGLSMIIAPVIAILGWIGMGLIVLDGASPDDPAKWMSE
                     LGANSEVVKFVMPLITVLFLIAIGGLNRIKNSMEGGEGHMMAGFGFLLVILGSAAQLG
                     EVAFTVATAEAVNTNMSVAPAMLASGQAIGAISTAITAVGFGLIGVGILQQKNFTPLV
                     AGLMIIAGIFGTAMSLIDYESPLMMIGYLGMVASLVWLGASLLRQED"
     gene            complement(7182..8348)
                     /locus_tag="QF838_06180"
     CDS             complement(7182..8348)
                     /locus_tag="QF838_06180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJO60302.1"
                     /translation="MNKSYDALIVGGGVMGCSIQYNLAERGMTRTLLLEKDVLGSGST
                     SRSQAILRMHYSNPETAMMAWESLKIFRNFEEITGVSSGYKKTGYLLAVPQHYRTPML
                     ENLQMQQRLGIDVNEVDSRFVQDLGLNAYPEEAESYAWEPLSGFADPYSVTLGYANRS
                     REFDCEIQVESKVKGINIDRDKVVGVKTADGDVDSDKVVVAAGPWSAPLLKELGLDFA
                     IETVRHQVFLLDRGEVPANRIPIIGDVSLNFSARPDIGNVIMVGVGEDEVVGPDEYNQ
                     SVDTDMLVKTQADFARRIPGLLDASFVGGWSGLFTVTPDWHPILSSIEGIEGLYVAIG
                     FSGHGFKLSPMIGQVMSEIILGEQSKSVDVSSLGANRFKEGQLMKSRYDMQVLA"
     gene            complement(8435..9562)
                     /locus_tag="QF838_06185"
     CDS             complement(8435..9562)
                     /locus_tag="QF838_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60303.1"
                     /translation="MDYGIAIPSYINAWKDVQAAEKAGFTHAWFFDSQLIYSDVWATM
                     ALAAENTEKIKLGTLVTIPSNRIVPVTAAAAATINKLAPGRVIVGIGTGYTGRNTMGL
                     PALKMKEFTTYVSQLKGLLAGKDVLFKEGDRERWIRLIHSKENSGRDDFFNIEDPIPV
                     MVAANGPKGQQLVGEISDGWITTGRSLNVPEGMPTVMESAKRSGNSFDRFGGTDIKPY
                     VTVLTSSCVLREGETVSSERVIRNIGPTLVPGVHAMWESSFGPGSNLGMSNSDIAEGY
                     NKYITEYGDKKTPSTPSDRRYLDVHEGHYAYLKEGEEKFVSSDLIARTLTGTGREINE
                     KLDHLESIGVNNVALSVVDSRSAQELIKDFGEKVILERRTS"
     gene            complement(9594..10010)
                     /locus_tag="QF838_06190"
     CDS             complement(9594..10010)
                     /locus_tag="QF838_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJO60304.1"
                     /translation="MNLDPFDKEQLFSDPNVAILATVDNKNRAHGMPIWYMYQYGKFV
                     MTAGKRSQKTRNIERMGKATLVIDRREPPYHAVMIRGRAEIGPAPGATWLLRLAIRYL
                     GEERGKTYFQGLGDDIVTITLYPDDVIEYLGVSGRA"
     gene            10255..>13177
                     /locus_tag="QF838_06195"
     CDS             10255..>13177
                     /locus_tag="QF838_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HJO60305.1"
                     /translation="MKTVVRTAVLSLILSSAFLIACGPSVGRATGLTEPQPPATQKLP
                     NPEIASSINLTSVPENEISSRLNKEITDGIWLLGTDIEPGTYMSENHNNSCDWARLKG
                     FSGDIDDIISNDNPIGRTIVTITPSDAGFSSQGCGTWVFIEPYATFEPLVPVTSPIAV
                     PAATLTPTPEIAGASVPHSPMRIQPTQTMISTRTKVPAPTATMNPTKTRVVVLTQTST
                     PIQTATPAPTLTPTPTPTFTLTPTPTLTPTPTPTLTPTPTPTLTPTPTQTPTPAPPPI
                     EQIKDGSLAGLGIKNEAYIEYSGDIDEWIFVPSSGMINTPISISVSTDNPDADGYLEF
                     ISPSGDVIGADDDSGYNMMPFVSNVLLPESGVYTVIFMLLDGSYGGYTLLVDTFDPES
                     PSQPLTPEATSTPVATTSSNTTEIVDPLSSRNGSDAINPLIKYSAEAGDKIVDQSSLS
                     RYDQNNYLNYDSFDFFGKYQGNNAQDLIPKPDPTDTVSLPSNFPILGNVFTIEVQVYS
                     AAHPNMGHRTIIGNGANPAKSERDRPPTITFNKVNGVNQVRYGFGVGPDQKGKRRIVN
                     TDMAEDQWYHIAFTFDGTTTKLFIDGVEIDSSDFISGLIPHPVPISVIGRKFLGKMDE
                     VRIWNIARTQEAIQSTLTSKLIGDEPGLIAYYPMDVDQNWELIDKGPNNHHATITNVE
                     ILHRHSSLECPNANGTLACPYPTIREALDSAQPGDTIFIKEGRYPEVIFKELFNQSYE
                     TNGPKITLRGESANVILDGTVPLIANWELVNGRYEASVDMNQLSTAAGAKVENIYALW
                     VDNRYMIPAMPVNFTNPTDVSTSTQNNREIGTVFGLNLTSPYYLPGEHTLEVQDEYIV
                     GDIDNLDAKEEWSYDEVNKKLHLIPGDKIPDSVNVRVRVRTKIVNMEFSDNLEFHNLH
                     FFAGSFDFLRASYILFEGSKFSHSWEAGISYLRPGYDADWQRGNAFKGGTNNTVRNSI
                     FQ"
BASE COUNT         3636 a         3184 c         2634 g         3723 t
ORIGIN      
        1 atcacgaaga gtgatttttc gtcgtgctga ttctgccact tctaaatcat gcgtaattaa
       61 aattacagtc gttccatctt cattcaattt ggtaaacaac tccatcaacg atgctgtatt
      121 ctcagagtct aggttaccag tcggttcatc tgccaaaacg aatgatggat tgtgcaccag
      181 cgctctggcc actgccacac gctgctgttc ccctccggat aattcattgg gcaaatgatt
      241 ggcgcgatgc tcaagagcaa ctcttcccaa catttcaaaa gaccgttccc ttctttcatc
      301 aaggggtatt cctgaataca aaagcccgtt ggcgacattt tccaaagctg aaaatccagg
      361 tagcaggaaa aattcctgaa atacaaaccc tatctgctga gacctaaccc ccgacaattg
      421 tttatcagac ataccgacca catttcgacc atctataagt attgaaccac tactcggaac
      481 agaaagggtc cccataatgt gtaaaagtgt agttttacca cttccagaag atccgattat
      541 gcccaacaac tcaccatgat tgacagacat cgatatatcc gacagggctt gaacaatggg
      601 ggggccgggg tagattttac ttatttcttt taactgtagt gcgaattgtg aggatctatt
      661 ttccagatta atcatgtggc tatcgtttat tggctaggaa ctactatctc tgtgccaggt
      721 tgcaatccag atccattgat ttcaacccat ccatcagcat atatacctgt ttcgacaggt
      781 acgagaatcg ttccagtttc agttacaatt tcaagagcat agcccccgga taataaagcc
      841 agcaatccgt tcactggtga agccagcacc cctttggcca attcttttgt tgcatttaca
      901 gttactagtg cacctgtcca tttaaaatat tctgcgctgc cttccaccgc aatcgaaacc
      961 tccaggaaag gatctccatc ttggggaaca attgcaaaac taccgacttc atctactact
     1021 cccacaatgg tggattcatc aggcagctca atttccactt ctgatccctt aaccactaaa
     1081 ttttgatcag ccacgtccac ttggatttca actttttgta aagatttact tgaaatgttt
     1141 atcgcaccat tagaactaat ttgggtcgat attctttcaa tgggagttag cgaaaatagt
     1201 tcggtcccag tatttatttt tccaccaatg tttggcaaag tagaagagaa attcaccatg
     1261 gacgttccag aaacgaatgt tatttcccca aaatttattt ctcctgacga tatcaggttt
     1321 aggtctgact ggagtttcat aatcgccgct gtggtatttg aaccaaatgt cgaatccatc
     1381 tccagtgtct cagtagttcc ataccctaaa gcaattaaat tcagtttcaa ttgatttatg
     1441 tcttcaccgg gtgctatacc ttctttcatg tctctccatg ccgaaagttc tccaaacata
     1501 aggagagaag ctgaatatcc ttctataaga tcctgtctat tctgcagtgc tatattcaaa
     1561 ctagaagttg cggaatttag tgattgggtt gcctgaagga gctcagcctc cgtcggaagt
     1621 tcgttcagtg ctaatctttg ttctttcgaa gattgaagag aaagatttgc ctgaagaagc
     1681 tcagcctcag tcggaagttc gttcagtgcc aatctttgtt ccttagcaga atttagtgtt
     1741 tgggttgcct gaagaacctc agcctcggtc gatggttcgt ccaatcctaa cctgctactc
     1801 aaaacttgtt ctagattttt ttctaaaacc cctaaagatt caagtgcagc ctcataattt
     1861 ttctgagcat tcaataaact attagcctct gcagacaaaa tattatttga aatcatgtcg
     1921 atcaaagagg atattaattt atcggggaaa acttggcctc tcaggttatt cgggcatagc
     1981 caggatgcct catcttgctt gtcagcgctg caaagagatt ggcgggcaat cagccgagcc
     2041 gcatctgttg atgttatggt gttttctcct tgggtcaagt ttgattctgc ttgggcaaca
     2101 ttcgcatctg cagaagcaat ctgggcagcc gtggccggcg aattgagatt ctcaagagtc
     2161 agctccgcct gggcaacatt cgcatccgca gaagcaatct gggcagccgt ggccggcgaa
     2221 ttgaggttct caagagtcag ctccgcctgg gcaacattcg catccgcaga agcaatctgg
     2281 gcagccgtgg ccggtgaatt gaggttctca agagtcagct ccgcctgggc aacattcgca
     2341 tccgcagaag caatctgggc agccgtagcc ggtgaattga ggttctcaag agtcagctcc
     2401 gcctgggcaa cattcgcatc cgcagaggtg acttgttgat cggctgccag taattcagaa
     2461 tcctctgggg attgatatat tctgtaaatt tctgcccccc gtgttaagcc ttgcccttca
     2521 ggtgccaaat aggtcagtac tccttcacct gttgtgatta tatgaacgtc gtcctcatat
     2581 ttaagtgtcc cgctaaaact ttcgtaagtt ttcaggtttt tttgctcaag aagtacagta
     2641 ttaaaatcag agttgatcgc ttgagtttgg tcattttgac tgaaatatgt acccttagcc
     2701 ataaaagctc ccaccgcaac cgcaattaca agtatccccc cggctacaat caaacccaat
     2761 ttccagtttc tcatcatttt tttcactagt tcaagccctt ggttagggcg attaacttaa
     2821 caaaatttag atcaaattca cggctgttta tatgtttcct tcagcgagtt atttgtaggc
     2881 ctatcttccg ggaggcccac cccttactcc cggtggcctc gcagcacctg cagaagcgtc
     2941 atttccaaag atggcctcag agcactgagc catggcttct tggagagact ctcggtccaa
     3001 actaaggtct cccagcattg attgaaatac ttggcctcgg ccccctgaaa aatctgggtc
     3061 tttcgcatcg ataccggcat ctctaagaca ttgagcaatt tccaaaaggt tgtcatttag
     3121 ttcgatttca tcctcttggg agcgctgtcc tgcaaagctg gcattttgaa gaataggaac
     3181 acatttttgc atcgattgcc gagtccttgg attttgaaaa ttaaagttag gatcattagt
     3241 caaactttgg cgaaaagcca tcatatttac agttccgtct gcatttagct caggatcaga
     3301 tacattcatc ccttctgttc gtaaacattc tgcaaaagaa gtcgcgattt cttcatcggt
     3361 ttgttccgga ccattttcag ccggggaagg caagtcggat aaagagccat ccggacccaa
     3421 tggattggcg gtatttgtcg ggatattttc agcagtcatt ttctggctgt tctggtccag
     3481 ctgggcaact cccgtagatg tatctttcga actgcatccc ataagcaaga cgcatagaag
     3541 gcaaaaaatg agggtgggag ccataacagt gaagggatct ttttttattg tgactttcat
     3601 ttgttttcac ttatgcctgg aaaatttatt ctgaccatct tactagtaga cattaagaaa
     3661 aacataagaa atgcagacat tatagagtca ttggagaaaa gctaataaat tgatcttctg
     3721 tgaccagaac attaatctca catgtggagt tatcctctcg tccaatcagg ccaggtacaa
     3781 aaacggtatg tcagggtggt tttgaagtaa agtcctaata tttgagatcc gccatctttg
     3841 gcataacttc attggcaagc aattccatcg acctctgcca tttatccaaa ggggaccact
     3901 cgtgacccat tgccaacaat actccgaacc ctcccacctc ttcgtataag gctcgaattt
     3961 ttgctacgac ctcatctgga tctccaacga tccagacatt ttccatgaca tattccgggg
     4021 tcacatcctc gtcgggcatt tcttggtctc gcttgtacaa accgagcatt cctagcttgg
     4081 acatcaatct atgaaaataa tcatcaaaat ctcttctaag aatgccattc aaagcttctt
     4141 ctcttgcagt ttttccatct tcagccacaa atatctctct ggctatcctc caatcagatc
     4201 gattagctat gttcttagtt tttgtagcgc ctgattcaac tgcatcccag tgtcctgcta
     4261 gagtattact tgggacaaga tttatactca ttggaatcca acctctctca cctgccatgg
     4321 tgagggtttc cgactttgga gaaacacttg ctaccccaat aggtgggtga ggtttctggt
     4381 agggcgacag gtgaaacttg agtcctatgt catcatcagg ttcgggtatc gtgaaatccc
     4441 aatggtcgct tttgtatctt ccaggctttg gatcttccca aagctgcaat atcagttcga
     4501 cagcttcttt tgacatattc ctatgagtac cgtcggaagg gtcaaaccca aaaacctcaa
     4561 aatcaccggg gaatcctcct gatccgatac cccactggaa tctaccatgg gcgaggtgat
     4621 caagcagggc tattctatgt gcaatcatga aagggttgtg attgggcata caagtgaccc
     4681 ctgtccccaa aataatcttt tccggcatag caatagcctt tgcaataaaa aggtcaggag
     4741 caggtatgtt ctcccactga gcggtgaaat gctccccaat ccaagcttca gaataaccaa
     4801 gcttatctag gtataccaac tggtccaaat catcatccat ggtttgtgtg atgttagatc
     4861 ccggaggatg aagaggcatt gtgaaacatc cgagtttcat ggttctttcc ttaatcaact
     4921 cttttcatat gaatacagga cctgttgcgg cagtatatga caggagtcat atcccctcaa
     4981 ctcgctaaat actcccacat tttatgtgtc ccatgatccc tggaaaaccc gctcataagc
     5041 caagggccat ggtggacgca aaaagcctaa agctcgtaca tcttcaagcc aaaatccgta
     5101 tggggcatgc ctagagtagg cacctccacc gataatcttg ctgattcgca gcagcaagga
     5161 ttcggcaagc tcagccactg cttctttagc caacagagtg ttccgagcgg gagcacttcc
     5221 tctttctact tcacgaagca tgccctcata cgattgttga accatccatg cgtcaatttc
     5281 tactttcgcc cactccactt cttcatagga acgcatacga tgacgtttag agtctaactg
     5341 ctgccgtgcg gttgctacag ccacttcgac aatgcctgta atcactgcca caaagaaggc
     5401 cccttgtggg cgatccatac ctgcaataat tttgcgtcgt tcatctccgg gaaatgccaa
     5461 tcttgtggcg tgaaaattct caaaaagcat tcgatggctt tgcgtcgacg tcataccatg
     5521 accatcccat gctgctaaaa gctcaacacc tgaagatccg tcccagtccg cattacgcat
     5581 atccaaaacg aatgtatctg gagatgtttc tccctctggg acggcatgag tcaccatgaa
     5641 agaggtcata cccgacccac tcccgaaatg cttctgcccg cttatctgat aatgaaaacc
     5701 atcctcagct aactttgctt cagaagtcga ttgagtatta tcccctccac taccgggttc
     5761 agacattatt gttccccaaa agtgtccctc cctcgctgtt tggaaagccc agtcacgctg
     5821 ttcttcccat gctttagtat aaggttcagg ggcggtcgat atagtcgacc aaccagcaga
     5881 gccaatcaca gcagggtgca ttgctgaaac aagagctatt gaggaatctc cctgagcaag
     5941 ggttttgaga atttcacaga ctttccgtgt cccgacgttg tctccttccc agattcctcc
     6001 aaattcagta ggcaccgcca ctaatgggta gccggactcc ttaagcctat cgaagtcttc
     6061 attctgtaac ccacgcctcc gctgcctttc ggatcgttgg gacgcaaact cacatgctat
     6121 ttcaccgaca ttgtccaaaa tggattgagc atcagcagct gaaccaccag tcattttacg
     6181 cattcatgtt ctccaagtat tagtacatgt gactatagtc atgatagaag gcaagtacaa
     6241 ctagatgatt ccttcaaata tcactctatc taacattcag aggcagacaa gaattcccct
     6301 acctaacacc cttacataga ctgaaagtca tgatttcctg ttattaatat tctaaataaa
     6361 aagggattaa tttccatagg ccgttttttg ggtctccgat atactccgcg atattagata
     6421 tcacgatatc agatacggag gttaatatgg attctcgtaa aattacaggt ttatcgatga
     6481 taattgcccc ggtaattgca attctggggt ggataggaat ggggttgata gtgttggatg
     6541 gagcatctcc agatgatccc gctaaatgga tgtccgaact cggcgccaac agtgaagttg
     6601 taaagttcgt catgcccctc ataaccgtat tgtttttgat agcaataggc ggattaaatc
     6661 gcataaaaaa ttccatggaa ggtggggaag gccacatgat ggccggtttc ggctttcttc
     6721 tagtaatact tggctcagct gcacagctag gtgaagtcgc attcacagtg gctactgcag
     6781 aagcggtaaa taccaacatg tctgtcgctc cagcaatgct cgccagcggt caggctatag
     6841 gagcaatttc cactgccata acggccgtag ggtttggttt gatcggtgtc gggattttgc
     6901 aacaaaagaa tttcacccca cttgttgccg gtcttatgat catcgcaggt atatttggaa
     6961 ctgcaatgtc cttaatagat tatgaaagcc cattgatgat gattggatac ctcggcatgg
     7021 ttgcttcgtt agtctggtta ggagcaagtc ttttaagaca agaagactaa cctctagaaa
     7081 cattatccga aacacagata acaatatggt gtaattccgg actatgcgct agccgtaata
     7141 tttcggagtt acatctttac tggactgata gaggaaccga atcaggccaa cacctgcata
     7201 tcatatctag acttcatcag ttggccttct ttaaagcgat ttgcaccaag agaagaaaca
     7261 tcaacacttt ttgattgttc accgagaatt atttcagaca tcacttgccc tatcatcggc
     7321 gaaagcttaa acccatgacc actaaagccg atagcaacat acaatccttc aatcccttca
     7381 attgaactca aaataggatg ccaatccggt gttaccgtga acagacccga ccagccccca
     7441 acgaaactcg catctagcaa gccaggaatc ctcctagcaa aatcggcttg agttttcact
     7501 aacatatctg tatcgactga ttgattgtat tcatccggtc caactacctc atcttcacct
     7561 accccaacca tgatcacatt tcctatatcg ggtctagcag agaaattcaa agacacgtca
     7621 ccaataattg gtattctgtt agccgggact tctcctctat caagtaggaa cacttgatgc
     7681 cgaacagttt ctatagcaaa gtccaaccca agctctttca gcaaaggtgc tgaccatgga
     7741 ccagccgcta ctaccacttt gtctgaatca acatcaccat ctgcagtctt aacccccaca
     7801 accttgtcac gatcgatgtt tataccctta actttgctct caacctgtat ttcacaatca
     7861 aattctctag aacgattagc gtaacccaaa gtcacactat agggatcagc aaacccggat
     7921 aaaggttccc aggcgtacga ttcggcttcc tctggatatg cattaagacc cagatcctgt
     7981 acaaatctgg aatcaacttc attaacatct attcctaatc gctgctgcat ttgcaaattc
     8041 tctagcattg gagtgcggta atgttgtgga accgcaagca aataacctgt cttcttgtag
     8101 ccagaagaaa cacctgtaat ttcttcaaaa ttacggaata tttttaaact ctcccaggcc
     8161 atcatcgcgg tctcagggtt agagtaatgc atccgtaata tagcctggga tcttgatgta
     8221 gaacctgatc caaggacatc cttttccaac aacaacgtac gagtcatacc cctctcagca
     8281 agattgtatt gaatcgaaca ccccatcacg ccgccaccta cgataagagc atcgtaagac
     8341 ttattcactc tattctccct acttctctga actcccgaat ttgtaaattg taatgcgatg
     8401 tagctgagtg gatgaaattt tgaaaatcct ccatttaaga agttcttctt tccaaaatca
     8461 ctttttctcc aaaatctttt attagctctt gagcagatct tgagtccaca accgaaagag
     8521 cgacattatt cactccaatt gattcaagat gatcaagttt ttcgttaatt tctcttcctg
     8581 ttcctgttaa cgttcgagca ataagatcgc tcgacacaaa cttttcctct ccttccttta
     8641 gatatgcata atgcccttca tgaacatcga gatatcttct gtctgatgga gtggatggcg
     8701 tctttttatc cccgtattcc gtgatgtatt tgttgtaacc ttcagcaata tcagaattag
     8761 acatacctag attggatccg ggaccgaagc tgctttccca catagcatga acgccgggta
     8821 ctaaagtggg ccctatgttc cttatcaccc tctcgctaga gacggtctcg ccttcccgaa
     8881 gaacacagga tgaggtcaac accgtcacat atggttttat atctgtacca ccaaatcgat
     8941 caaatgagtt accagatctc ttggcacttt ccatgacagt gggcatacct tccggcacat
     9001 ttaaggaacg accagttgtg atccagccgt cagatatttc ccctaccaac tgctgcccct
     9061 tcggaccgtt tgcagccacc attaccggaa taggatcctc tatgttgaag aaatcatcac
     9121 gaccagagtt ttccttacta tggatcagcc ttatccatct ttctctatct ccctctttga
     9181 acaggacatc ttttccagcc aataaacctt ttaattgaga tacataagtt gtaaattctt
     9241 tcatcttgag agcaggcaag cccatcgtat ttctacctgt gtatcctgta ccaataccca
     9301 caattaccct gcctggagcg agtttattta tggtagctgc cgcggccgct gttaccggca
     9361 caatacgatt gctaggtatt gtaaccaagg tacccagttt tatcttttct gtattttccg
     9421 ctgccaatgc cattgttgcc cacacatctg agtaaatgag ttgggaatca aaaaaccatg
     9481 cgtgggtgaa gccagctttt tcagcggcct ggacatcttt ccatgcattt atatatgacg
     9541 ggatagctat tccatagtcc atatcaacac ctactctata tgttctttag tagctaggct
     9601 ctacccgaga caccaaggta ttcgatcaca tcatctggat agagtgttat agtcacgata
     9661 tcatccccca aaccttgaaa atatgttttc cctcgctctt cccctaaata acggattgcc
     9721 agtctaagca accaggtagc acctggtgct ggtccaattt ccgctcgtcc gcggatcatg
     9781 actgcatgat agggcggttc ccggcgatct ataacgagag tagccttgcc cattctttcg
     9841 atatttctag tcttctgtga ccttttgcca gcggtcatta cgaattttcc atattggtac
     9901 atgtaccaaa taggcattcc atgggcccga ttcttgttgt ccacagtcgc cagtattgcg
     9961 acatttggat ctgaaaatag ctgctctttg tcaaatggat ccaagttcat cggcgtacct
    10021 cctacatcta ggatactaac aggacttgaa cctctgtctc taggttagga attgcaccaa
    10081 aagggggttt cagtggggca tattgaggat taacattact atgcgcctat tttgaactaa
    10141 gatcaaaata attattaagg ttgctcaata cgtattttca ctcttgtgtg gtttttaatg
    10201 tggaattata gtctttggga ttatgcttcg gaaaaggaga tttaagtggg tgatatgaag
    10261 actgttgttc gcacagcagt tttatccttg atattgtcca gtgcttttct gatagcttgc
    10321 gggccatcag tcggtcgggc taccggcctt actgaaccgc aacctcctgc tacccaaaaa
    10381 cttcctaacc cagaaattgc aagctcaatc aaccttacat ctgttcctga aaacgagatt
    10441 tccagcagat taaataaaga aataacagac ggaatctggc tgcttggaac agatattgaa
    10501 cctggcacct atatgtcaga aaaccacaac aacagttgtg attgggcgag actaaagggt
    10561 ttttcaggag acatagacga cataatatcc aatgataatc cgattggtag aacaattgtg
    10621 acaataactc ctagtgatgc agggttcagt tctcaaggat gtggcacctg ggtgtttata
    10681 gaaccatacg caacctttga gcctttggtt cccgtaacgt ctccaattgc cgtaccagca
    10741 gctacattga ctccaactcc cgaaattgct ggagcttccg ttccgcattc accaatgaga
    10801 attcaaccca cccaaaccat gatttccaca agaaccaaag taccggcccc taccgcaact
    10861 atgaatccca caaaaaccag agttgtggta cttactcaga cctctacacc tattcagacg
    10921 gctacgcctg cacctactct gacacccacc ccgactccta ccttcacact caccccgact
    10981 ccgactctga cacccacccc gactccgact ctgacaccca ccccaactcc gactctgaca
    11041 ccgactccta ctcagacccc tactccagcc ccacctccta tagagcaaat taaagatggt
    11101 tcattggccg gattaggtat caagaatgag gcttatatag aatattcggg agacatagat
    11161 gaatggatat ttgttccaag ttcgggaatg ataaataccc caataagcat ttccgtgagc
    11221 acggataacc ccgatgcaga cgggtacctg gaattcattt caccttctgg tgatgtgata
    11281 ggagcggatg acgatagcgg atataacatg atgccgttcg ttagcaatgt actgctaccc
    11341 gaaagtggcg tatacactgt tatatttatg ctattagatg gaagctacgg tggatataca
    11401 ctactagtgg atacgttcga tccagagtct ccttcacagc cactaacacc tgaagccaca
    11461 tctacaccag tggcaaccac atcctcaaat acgacagaaa tcgttgatcc tttaagcagt
    11521 agaaatggta gtgatgcaat taatcccctt atcaaatatt ctgctgaagc cggggataaa
    11581 attgttgacc agagttcact gtctcggtat gaccaaaaca actatcttaa ctatgattca
    11641 tttgattttt tcgggaaata ccagggcaac aatgctcaag atttaatacc taaacctgat
    11701 cctaccgaca ctgtttctct tcctagcaat tttccaatcc tcggaaatgt cttcaccata
    11761 gaagttcaag tctactcagc tgcccaccca aatatgggtc atagaacaat cattggcaat
    11821 ggtgccaatc ctgctaaaag cgagcgggac aggcctccaa ctatcacatt taacaaggta
    11881 aatggggtaa accaagttag gtatggcttt ggtgtcgggc ccgaccaaaa gggaaaacga
    11941 agaattgtta ataccgatat ggctgaagat cagtggtatc acatcgcttt tacctttgat
    12001 ggcaccacga ccaaattatt cattgatggc gtagaaattg acagctctga ttttatatca
    12061 ggtttaattc ctcaccctgt acccatttca gtgatcgggc gaaaattttt aggaaaaatg
    12121 gatgaggtac gcatttggaa catagccaga acacaggagg ctattcaatc cacattgaca
    12181 agtaaactta ttggtgatga accaggattg attgcttatt atccgatgga cgttgatcaa
    12241 aattgggagt taattgataa aggtccaaat aatcatcacg ccactattac aaatgttgaa
    12301 attttacatc gccatagctc attagaatgc cctaacgcca atggcaccct ggcctgtcct
    12361 tatccaacaa taagagaagc attggatagt gcgcagccag gagataccat atttataaaa
    12421 gaaggtagat acccagaggt aatcttcaaa gaattattca atcaatcata cgagacaaat
    12481 ggtccaaaaa ttacactcag gggtgaaagt gcaaatgtca tattggacgg cacagtcccg
    12541 ctaatagcaa actgggaact cgtgaacggt agatatgagg cttcagttga catgaaccaa
    12601 ctgtcaactg ccgccggcgc aaaagtcgaa aacatttacg ctttgtgggt agataacagg
    12661 tatatgattc cggcaatgcc tgttaatttc acaaacccta cagatgtttc aacaagcact
    12721 caaaacaatc gggaaattgg cactgtattt ggtttaaatc taacttctcc ttattacctg
    12781 ccaggagaac atactttaga agtgcaagat gagtatatcg ttggcgatat agataacctg
    12841 gatgctaaag aggaatggtc atatgatgaa gtaaataaga aattgcatct tattcccggg
    12901 gataagattc cggattcagt aaatgtgcga gtgagggttc gtacaaaaat tgttaatatg
    12961 gaattttcgg ataatttgga gttccataat ttacattttt ttgcaggctc atttgatttt
    13021 ttaagggcta gctatatcct ctttgaaggt tctaagttct cccatagttg ggaagccgga
    13081 atcagttacc ttcggccagg atacgatgca gactggcagc gagggaatgc ttttaagggt
    13141 gggacaaata acaccgtaag aaattctatt tttcaat
//
LOCUS       DASCNS010000171         1277 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001338302, whole genome shotgun sequence.
ACCESSION   DASCNS010000171 DASCNS010000000
VERSION     DASCNS010000171.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001338302"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="QF838_06200"
     CDS             complement(<1..1057)
                     /locus_tag="QF838_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60306.1"
                     /translation="MKRSAYFSIVVFLSVMVSACTEINETERAPTVERKIVETPASGK
                     IVSRVTPNPSKSTNQTPRETSRVLPDYSGSNDPQVEEQFLSFRTQLDYLISNPKSVDD
                     WIDMISLDSNSLFLPELPDCRGSKVRKCFDELLPLIHLAMNADYAPGSLAKDIPPDNA
                     CTVMNYTYGIVKVYGEPQSVGYPETNPNFETMVRFNFIDEAELSDFRWAFQHRDDWDY
                     LLHCVKPRETSRVLPDYSGLNDPQVEEQFLSFRTQLDYLISNPKSVDEWIDMISLDSN
                     SLFLPELPDCRGSKVRKCLDELLPLIHLAMNADYAPGSLAKDIPPDNACTVMNYTYGV
                     VKVYGEPKSVGYPEINSN"
BASE COUNT          372 a          274 c          268 g          363 t
ORIGIN      
        1 aattagaatt tatttcagga tatccaacac ttttgggttc accataaact ttcacaaccc
       61 catatgtgta attcattacg gtgcaagcgt tatcaggagg tatatccttg gcaagtgatc
      121 ctggcgcata atctgcattc atggctaggt ggataagagg gagcagttcg tcaagacatt
      181 tacgaacctt gcttcccctg caatcaggta actccggcaa gaaaagtgag ttgctgtcga
      241 ggctaatcat atcaatccac tcgtctactg acttcggatt ggagatcaaa taatccaatt
      301 gagtccgaaa ggataaaaac tgttcctcga cttgagggtc atttaacccc gaatagtccg
      361 gtagaaccct ggaggtttct cttggtttta cacagtgaag tagatagtcc cagtcgtctc
      421 tatgctgaaa agcccaacga aaatctgaca gttcagcttc atcaataaag ttgaatctga
      481 ccatggtttc gaaattggga tttgtttcag gatatccaac actttggggt tcaccataaa
      541 ctttcacaat cccatatgtg taattcatta cggtgcaagc gttatcagga ggtatatcct
      601 tggcaagtga ccctggcgca taatccgcat tcatggccaa gtggataaga gggagcagtt
      661 cgtcaaaaca tttacgaacc ttgcttcccc tgcaatcagg taactccggc aagaaaagtg
      721 agttgctgtc gaggctaatc atatcaatcc aatcgtctac tgacttcgga ttggaaatca
      781 aataatccaa ttgagtccga aaagataaaa actgttcctc gacttgaggg tcatttgacc
      841 cagaatagtc cggtagaacc ctagaggttt ctcttggtgt ttggtttgtc gatttactcg
      901 gattaggagt aactcgagaa acgatttttc cagatgctgg tgtctccact atttttcttt
      961 cgacagtcgg tgcacgttct gtttcattta tttctgtaca tgcagaaacc atcaccgaga
     1021 gaaatacaac tatcgaaaaa taagctgacc gtttcatgga gccattataa ataatttaaa
     1081 aatctgtgtg tgtcatcctt gaaatggttt gactgaaaga ttgatctagg gttcaaataa
     1141 acaaaattaa gagctttcct taaaggaggg ccactcgctt ctttttaaat agatcccaat
     1201 cccactctga gccccaacca ggtcctgacg gaggagtcac ccaaccggat tcaggactaa
     1261 ttgggttgag caaacca
//
LOCUS       DASCNS010000172         1281 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001341749, whole genome shotgun sequence.
ACCESSION   DASCNS010000172 DASCNS010000000
VERSION     DASCNS010000172.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001341749"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1281)
                     /locus_tag="QF838_06205"
     CDS             complement(<1..>1281)
                     /locus_tag="QF838_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HJO60307.1"
                     /translation="YDHNTGQVRIITVWPEVNIGYGAENMKYRFQWTYPISFSPHDST
                     VLYTAGNIVFRSLDQGSNWTPISPDLTRHDREKLRPSGGPVTLDTSGAETYSTIFSFA
                     ESPIEKGVFWAGSDDGLVHVSKDEGDSWQDITPKELPEWSLISMIEPSPHSAGTAYMA
                     ATRYKLGDNSPMLFKTEDYGKQWKVINGNLREDDFTRCIREDPVKPGMLYAGTETTLY
                     VSFDDGIRWRDLKGNGSVGKSSLPVVPIYDIAIKEDDMIVATHGRSFWILDDLTQLRQ
                     VPEIEGVNSPHILQPRDTIRIAAPLRNPKAVEGKNYQLSLGAAVTYTEDKNTYGEVER
                     SFLDAGQNPPAGVLINYFLPKEIHEELSLTFLDQSGNMIVTYSNKVAANSSPEEEDDQ
                     LSIASNIGMNRFIWDMRYPPANKVPDDKTTADRV"
BASE COUNT          297 a          306 c          289 g          389 t
ORIGIN      
        1 caacccggtc tgcagtggtt ttatcgtccg gcactttatt tgctggtgga tatctcatgt
       61 cccagatgaa tcggttcata ccaatatttg aagcaataga aagttggtca tcttcctctt
      121 ctggactact gttagctgca actttattag agtatgtgac tatcatattt ccagactggt
      181 caaggaaggt taacgacagt tcttcgtgta tttctttggg tagaaaatag ttgataagaa
      241 cccccgccgg tggattctgt cccgcgtcaa ggaaagagcg ttccacctca ccataagtat
      301 tcttatcctc tgtgtacgtg actgcggctc cgagtgagag ctggtaattc tttccctcaa
      361 ctgctttagg atttctgaga ggggcagcta ttcggatggt gtcccttggt tgaaggatat
      421 gtggagaatt aaccccttct atttcaggga cctgcctgag ttgtgtaagg tcatcgagta
      481 tccagaatga ccttccatgg gttgccacga tcatgtcgtc ttcttttatg gctatgtcgt
      541 agataggaac gacgggcaat gagctcttcc cgacactccc gttacccttc aagtccctcc
      601 accggattcc gtcgtcaaat gagacgtaaa gtgtggtttc tgtccctgca tataacattc
      661 cgggcttgac gggatcctcc cgaatacatc tagtgaaatc atcttcccgt aggtttccgt
      721 taatcacctt ccattgctta ccgtaatctt ctgtcttaaa gagcatcggt gaattatcac
      781 ctagtttgta tctggttgca gccatatatg ctgtgccagc tgaatgtggt gacggttcta
      841 tcatgctgat cagggaccac tcaggtaatt ctttaggggt aatgtcctgc catgaatccc
      901 cctcgtcttt ggatacatga accaacccat catccgaacc tgcccagaaa actccctttt
      961 caataggcga ttcggcgaat gagaatatgg ttgaataagt ttctgcccct gacgtgtcga
     1021 gtgttacagg ccctcccgat ggccttaact tttccctatc gtgtcgtgtg agatcaggac
     1081 taattggagt ccaattactt ccttggtcca gagacctaaa tacaatgttg ccggctgtgt
     1141 acagcacagt tgagtcgtga ggggaaaatg aaatcggata ggtccattgg aacctgtact
     1201 tcatattttc ggcgccatat ccaatattta cttccggcca gacagttatt atcctcacct
     1261 gaccggtgtt atgatcatat c
//
LOCUS       DASCNS010000173        21568 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001359864, whole genome shotgun sequence.
ACCESSION   DASCNS010000173 DASCNS010000000
VERSION     DASCNS010000173.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 21568)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21568)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21568
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001359864"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            2..142
                     /locus_tag="QF838_06210"
     CDS             2..142
                     /locus_tag="QF838_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HJO60308.1"
                     /translation="MTGASITALTIYDMCKAVDRSMNFRVWLATKSGGQSGNFVSEPP
                     SA"
     gene            167..1432
                     /locus_tag="QF838_06215"
     CDS             167..1432
                     /locus_tag="QF838_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013884.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HJO60309.1"
                     /translation="MNNSRTRYHGLDALRGTAMVLGIVLHAALPYIPGIPSSIWPSDN
                     SSSYPIKIVFEFIHMWRMPLFFMLAGFFANLIITRKSWMFWCNNRFLRVGLPIAVFFP
                     VMSLTLPWIWKYGQTGEFYFFYSNTGQPFHLWFLWHLLIFVLFSTLFRMLSKSFVALM
                     RLLNGNRLEVLTSFLYKLKNVIAVIIFKSKFPIGFMLACGITNLWAGGELIINPFGSG
                     LYFLFGFSLYKNPSLFSFIKREWKYYLLIAIFIFSLYIAFDMRGVKDITEVIYQTGIG
                     ENPAPDVSLFVLTRYSMETIGAVLFSIGFIGLAEDKFGSYSRFSRFISDGSYWMYLIH
                     LPVVAFTTFFMFGWPIYPEIKFVIATSFTAGVCLVTYRYFVRATFIGLFLNGKRHRGS
                     NFGNVCSACGTNLQNPERFCTECGIELSH"
     gene            1541..2752
                     /locus_tag="QF838_06220"
     CDS             1541..2752
                     /locus_tag="QF838_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60310.1"
                     /translation="MKITNIDTLIVDAGWRPWTFVKVETDEGITGWGECSDGKSPHGI
                     EGVIRDLKPVLIGKDPCAFEMRFQEMYIGTRASKGGIAAKALAGLDCAFIDIKAKSLN
                     ISVAELFGGPTRDKVRVYWSHCGSSRIRHTDILGTPPIETWDDVTRLGKEVKSKGFTA
                     LKTNVLLPGQNATFGGGSFGGAGSTDQIAPKWLIPHIETLIGTFRDAVGDDIDINLDL
                     NFHFKTEACLRIAKVLEKFDMLWLEIDMYDPLSLRQIKDSTDTRICTGENLFYMSDYL
                     PYFENHSADMFMIDVPWNGFSQSKKVGDLAEVFQHNVAPHNYYSHLSTFVSASLCASL
                     PNVRIMEIDIDDVPWKDDLTTSTPEVIDGYMTVPNKPGWGVDINEEVARAHPWDESKI
                     MSVQSTGYKVI"
     gene            2918..3169
                     /locus_tag="QF838_06225"
     CDS             2918..3169
                     /locus_tag="QF838_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HJO60311.1"
                     /translation="MNIYVGNISYGTGEDDLRDLFARYGEVSAVRVITDRDTGRSKGF
                     GFVEMADDDQAKEAIAALDSNDFMGRDLRVNEARPREPR"
     gene            3333..3803
                     /locus_tag="QF838_06230"
     CDS             3333..3803
                     /locus_tag="QF838_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015369.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HJO60312.1"
                     /translation="MNDVNASGLKTVGTEPYYGTFQGEKYILKYKDGTEKPVEEMQVK
                     STITSPAENESLKCCADYHISGNAWSGRTDIAKVEVSVDSGNSWHEAKLIGPSEQYSW
                     QQWNYKWTPNTPGEHILLSRATNSDGEQQPLESVWNELGYAVNGSKPIKIVVSS"
     gene            3840..4034
                     /locus_tag="QF838_06235"
     CDS             3840..4034
                     /locus_tag="QF838_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60313.1"
                     /translation="MSWVKEDPKYADIANVIKCMSINEEAMNAVWDMGNKISFGSSAL
                     TRAQEEVIATVVSSINHCKY"
     gene            4083..4406
                     /locus_tag="QF838_06240"
     CDS             4083..4406
                     /locus_tag="QF838_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60314.1"
                     /translation="MASIVMQDYGDAVLDDETLAIVEYVEKLTKEPSEMTENDIQTLR
                     KAGLSDNQILSIVMITAMFAFMNRLADGLGVEIEDEKGAFVNSWLRTSNNSSSWLHHQ
                     PRERV"
     gene            complement(4425..4619)
                     /locus_tag="QF838_06245"
     CDS             complement(4425..4619)
                     /locus_tag="QF838_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024139.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJO60315.1"
                     /translation="MVSNDLMTTKELADYLQLDRMTIYKLLKAGDIPANRVGHQWRFF
                     RSDIDEWIRSKKVDLNTIQI"
     gene            complement(4756..6240)
                     /locus_tag="QF838_06250"
     CDS             complement(4756..6240)
                     /locus_tag="QF838_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60316.1"
                     /translation="MLKLAYSSSNYKWLVFAAIGLGSLTNVIHHGSISIALPTIAREF
                     DVSLTTIQWVVLAESLTISALLLPMGRLSDIIGRKPMYLLGVVLFGSMALFSGSSPAI
                     AQYLGYAHPIMLMIPFRIFQGVGAAMTQANGMAMVTSVFSDRERGKGLGAHGSIIGTG
                     GIIGPIVGGFLVTYASWHWIFWINVPLCAITFLGTALLLDSSRFRGATQGDKSFDWLG
                     ATLSSSSLLVFLLTLSNGAELGWFSLPIMAGFLIFIGLITTFIWWELKSSSPMLDLSL
                     FRSRLFSVGIGSSYISFLGVTSFRFLITFYLQAALRLTPAQISWVLIPNAISRIILGP
                     LSGNLSDKYGTKLFTTLGLLLAGCGLLLMAFMADSTSIWFVILAIVIMSSGSGIFMSP
                     NSAAIFSSATGNKYGVTSALVNLSRNSGNVTGIAIATAIVSGTMISAGFSSNVEEVMI
                     AETGSRILSTFISGMRLVFLLMAILQFISSIGHLTTSRAPLSET"
     gene            6322..8043
                     /locus_tag="QF838_06255"
     CDS             6322..8043
                     /locus_tag="QF838_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60317.1"
                     /translation="MLTTSITGTPSEVLVEQLVASGIKYVFYNSGSREALFFDALNSN
                     PNIDGILALHEGSVASMAGAYTQVNSEPAVMVVHLGAGLAQCMGQLYNIWFGGLPVVV
                     ITFAGDTGSFTDRINLDLDSSYSPASISVPMVKETWTVIEPEGLPAAVDRALRVATTP
                     PFGPVHLAVYDKMLENQQVSTEIIHGGAKNIKAGNATDQDIVKILDALDKADKPLLYV
                     GDGVWKSGGDKLAAELATHFGTVIASPETDLRGISLKHPQHIGMSNTAFDSYEPDVII
                     SLGVRHQGSGKKEDFDPLRKTKQILALGSGLEYLKNYPGLEMGIIADEASSISRMLEI
                     AKNDYKADRYSKRREKTLTLVEQQRSKLRDSLMPSYEKGRVRATLLVDAIDTELEKIG
                     GGYITNEQFAARYDAVGPGLSERNNILMKGAGGSEGWGVGAGIGAKLAVGEKPVIGLV
                     GDGSLYYADSGLWTAVHHNVPLLYVIPNNGAYGIVAGFFGQSGGRMSDTGNYQGVVLD
                     GIDPVKIAEAFGMEGERVDDEDKLNQTIEKALEIVMKQNRPYLLDIRLPLGLPEGGVA
                     DQQYRMK"
     gene            complement(8097..9014)
                     /locus_tag="QF838_06260"
     CDS             complement(8097..9014)
                     /locus_tag="QF838_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60318.1"
                     /translation="MSTELAQIADSTPSLPIRLLSASLKFTARRPLAAAGLFIIIIMC
                     FMAAAAELITWYDPIEWRLFDKLQSPLSSSEREPFSTFLMGTDDKGRDIWAQMAIGAQ
                     VSLMVGFLSVAFGSGLGGVLGVISAWFGGKTDQIIQRAMDALMSIPTLILALAVTSVL
                     GQTIENIIVAVGVVQIPRANRVVRAQALTVKETQYVDAARAIGANNIRIMVQHITPQC
                     VAPFLIIATSALGIAILTEASLSYLGLGVPPPRPSWGGMLSGAARDYFILAPWMAIWP
                     GVGLSLSVYGFNLFGDGLRDALDPRLRGT"
     gene            complement(9036..10001)
                     /locus_tag="QF838_06265"
     CDS             complement(9036..10001)
                     /locus_tag="QF838_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60319.1"
                     /translation="MKEYLIRRLLFGVLTLFTVSALIFALLRIAPGDVALMIAEANSG
                     GEGIDVSEEDLEEIREKLGLNAPLHIQYGTWIKGYVVGDWGDSLFTGRDVFDEFKLKF
                     PVTLELVIFSQIIAIILGLPAGIIMALKQDSWLDYSIRVASLAGLSLPSFWSATLLLL
                     GGAYFFMWNPQLGSFAITEEPWQNIQQFFWPSIMLGYISASTKARMMRSTMLEVLRQD
                     YIRTAKAKGLTTYVVTARHALKNAFIPVITVIGITTALALGGSVVMERVFSMPGLGSF
                     ILEGMLARDFPIVQSITLFFAIAVVFVNLLIDISYGWFDPRVRYQ"
     gene            complement(10223..12235)
                     /locus_tag="QF838_06270"
     CDS             complement(10223..12235)
                     /locus_tag="QF838_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60320.1"
                     /translation="MRSKLIMMVGAITVLSLFMACAAQEAAAPAAPAAAAPAAAAAAP
                     AAAAPAAPAQPAAAAPAAPAPVALVQKEVAKEAKATAVPTPVQKAAEQTWMDLYMSGP
                     GYNSEWGQPIKGGIFRYGASHKLSGHDPNYGHSFEGPQFLPTYNALLRYDPWKGLGGE
                     IEGDLAETWEVQDGGLTVLFKLREGINFQVNPNLPAEIASAVSGDSFTCEDAQASLEY
                     ALNPPEGILHSGPRAALGHYEKSSCVDDYTFKVEFKEALARTLPQFAGMLGSPNNMDK
                     DFIAYIRQEGTHPDLEIAYIDMLDETTPETFLFGTGTGAFVPTEFEVDVQSKVRANPD
                     YWREGLPLTEGQDQFIITDATARFSALITGQTDYYGEGSASLLPAHVEQIKARFADKF
                     TIMPALHSWGKGIQINMEREPFNDAKIRMAMHLALDRDDWVDFNMAAGVSGVHRHTLW
                     MPPGSIWALPDSELGELPGWRRGDGKVADIAEANRLVDEVLGAGNRFSVECMAQSAQM
                     YIDGCLYFQDQMSRNLDVEVTIASVESAVQSTRGKEADYDIHYGSKVRTNVADPDDFY
                     MIHLVKDKESWYYRGTGRETADPELAARLEKLALEQSSELDVAKRVELVHDIERILAT
                     EAMYSIPYPWTYIFPAWSNEVKGWQLYPHPSQNKWAQWERMWLDRN"
     gene            12582..13928
                     /locus_tag="QF838_06275"
     CDS             12582..13928
                     /locus_tag="QF838_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HJO60321.1"
                     /translation="MPGEEGEAIEINRLQTLKRAKKKRYIFRGGLVAVVLIILGLLFW
                     NPGNQNFEPTTDISAQVTGFEWNMYQGGPALSGSRDLNQLAFLNFCLLFDCVSNSDSN
                     FGDIVWRYQANGAISSSPAVTDKAVYITTEDRRILALEIETGNPIWEYPAEAPTDTSP
                     AVAGDMLYVALREGKFVALDRNNGTVVWDFQSSEPFFSSPTIHNGLAYIGSGDKKFFA
                     FDALTGEQIWEYDAGSRIANAAAVNEDVVAFTTQNNVVHVVDALNGDLRLDYPIGSPY
                     SAPAMDGKLLFVADLNGVVRAIDWTKIQYPMEKLARRFRMTMFLWGFEDTPPVMKGFV
                     WKFRNPREVFQNTPTIAKGMVYVPSLNGSIYKLSATTGDIQWRYTTDGTISESVSVVG
                     DLMLTGDSTGKIHGVDVETGKKIWEMQLDGHISSTPVVSAEMLFVATQNGSLYAIK"
     gene            13952..14491
                     /locus_tag="QF838_06280"
     CDS             13952..14491
                     /locus_tag="QF838_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HJO60322.1"
                     /translation="MADVTADQFNSHIAEGKDLSGLNLSGLDLSNMLMQNLVLRGANF
                     TGCDLSRSDLSGSDLTRANLRGANLDEAILRNTIFTRANLTIVDMRGADLRCANFEGA
                     NLSGAPSNSWQSTARVIGANMHKANAQNANFSNSILVRVNFNSSNLYGANFEGANITR
                     VSLQDAKYDIDAFDRAIGV"
     gene            14556..15137
                     /gene="pth"
                     /locus_tag="QF838_06285"
     CDS             14556..15137
                     /gene="pth"
                     /locus_tag="QF838_06285"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HJO60323.1"
                     /translation="MDIGHHDPQLLIIGLGNPGPKYQQTRHNAGFWVVDFIVNALKLK
                     LKRSHKSTLLAEGSLRGLDIVVAKPRTYVNLSGESAAYLLSRYSLPTQKLLVVYDDIH
                     IGVGKIRLRANGSAGGHNGMRSIIDSLNTQDFPRLRIGVGKPDLGEDQIGYVLGKPTL
                     LEKETIGKSVAGALDCVETLVTENINVAMDRFN"
     gene            15156..17021
                     /locus_tag="QF838_06290"
     CDS             15156..17021
                     /locus_tag="QF838_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017368.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HJO60324.1"
                     /translation="MYLVQRQKHYLTVLFLLAFLVLSAVSCSQEAFSFGSDALENNKS
                     SVASNFETWVPLVAIPPDRDLENLSTQYGFGPDEMLLPVKSDDKNENVGKKEEFTVVN
                     LSLTESYTVEGELVNVTDNAYWYVDINSEIDRDLYNIVATKWEKIHSDYFATYHVPKL
                     TILNTALNGAAGYFSDVDSYPRWVHTNSNERPMVYIDPSRNKPGSDRYMSVLIHEYQH
                     FVNNEADQGEEAWVDEGLAELAVRNIGYETPLEKYFLVDPDTQLNYWSDDPRKTLAHY
                     GAASLFFEFVSGRLGGSQSLRSLIEEPLDGILGVESWLEKNDTHFHQEFGQWILANYL
                     GSETGQYSYPTRTLRLRSGVDELKPGENTFRVSQYGTRYFEIDVNGRNIDIRFDGSDF
                     VNRFKTECHTQCWWSNKGDSINSSMTASLDFTKKKESSLHFDLWHDIEDGWDYGYLSV
                     SLDEGNTWKTLNIPGTTSTNPVGSNYGNGYSGRSDWTSHIVHLDDYAGKKILVRFQYV
                     TDAAVHLDGMLIKAARLSENGEPSSSEHLKWIPSGFVLVDDILKQKFLVQIVKKMKSG
                     QYIIDGITLDDSNETLYTIREDENLDSVTVVVSGMTGLTQQPAVFDLEVSEIFAK"
     gene            17085..18572
                     /locus_tag="QF838_06295"
     CDS             17085..18572
                     /locus_tag="QF838_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60325.1"
                     /translation="MTVNNDKNVISINYKWLVYVSVAVGAFLTVSDQTGTNIAMPRIA
                     EDLSADLPTVQWVYLLYTLSISSFLLPMGRLSDLVGRKSVYCTGLAIFSIGALGAYLS
                     GILPILLAAKAFQGLGAAMVQANGMALMAESFPRNERGKALGLYMTIIGIGATGGPVI
                     GGYLIGAFGWRSMYLAILMFGVMALVLSFKILKKEEFTSRPKNLSFDWIGATLSASAL
                     ALFLLGLTYSYRLGWSNPVVIVGFLLSTISLAGFIWWEKRNQNPIVDLSLFSVPVFSL
                     GMAARYLAFMSGSSAFFLMPFYLIQVVGLKESEAALVLAPSSIMMAITSPLSGRLSDK
                     IGTKWPSVAGLVCWAAAVAVYTTLTVDSSPWIVVIGMFLQGSGNGIFGSPNTTAVMNV
                     VESNRYGVVSALLNMVRTSGNLTGIAMGTTLVVFTMASMNYPPDLSSIESVGATSYGE
                     GLKIAFTEGITRAFWIGTGIVGFAALFTAFRPEKPIKQTKTTGTR"
     gene            18651..19469
                     /locus_tag="QF838_06300"
     CDS             18651..19469
                     /locus_tag="QF838_06300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO60326.1"
                     /translation="MSGSDYSFSTFSKNEFYSRLNARLVDMAEIGSDLRVVDLACGTG
                     GVTRLILERINRTRDTVVIALDHSQTALKAAMEDLKDISDNAVKYVQSGVENVSEAVK
                     GSVDTIVFCNAIHYLPDKDKVVHDIGTALRPGGKFAFNTSFYEGGQHEESSAFYSKWM
                     FKAFRILRKEYGLKPNRAAKVESRRQLTPEEYSDLLERNGLTILKQKVDTVQVPIEGW
                     LDISTFSDFIEGTMPGVPMNEASAALQEGIRQTYSELNVTHVPRNWLDIIAVKR"
     gene            19843..21045
                     /gene="trpB"
                     /locus_tag="QF838_06305"
     CDS             19843..21045
                     /gene="trpB"
                     /locus_tag="QF838_06305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HJO60327.1"
                     /translation="MTLINELPDQSGRYGQFGGRYVPEVLMPAINELEESYRQVQSDN
                     KFQTELSQLLINFVGRPTELYFASNLTERFGGAKIFLKREDLAHTGAHKINNALGQGL
                     LARKMGKTRIIAETGAGQHGVATATVCAKLGLDCVVYMGEEDIRRQALNVFRMKLLGA
                     EVISVDSGSKTLKDAINEAMRDWVTNVNDSYYLIGSVVGPHPYPMIVRDFQKIIGLET
                     RKQILEQMGKLPDYLIACVGGGSNAMGLFYDFIGDKEVRLVGVEAGGEGIESGRHSST
                     ISAGSIGVLHGSMSYLIQNEDGQIIETHSISAGLDYPGVGPEHSWLNDSERASYVSVD
                     DSQALEGFKLMCELEGIIPALEPAHAIYYVSQLAPTLKKEQIIVIGLSGRGDKDMETV
                     ASEMNVQV"
     gene            21052..21450
                     /locus_tag="QF838_06310"
     CDS             21052..21450
                     /locus_tag="QF838_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60328.1"
                     /translation="MKYFAARLSHAILWRIPPVLLLIFFLGPSIGVMFNHHFPEQNPY
                     HPHFADGLKHSHSVLGEHTHEKANASPSYNVALLVKNVGISTSGIHIALICFETQVPS
                     PPFSLAKSLILTMDIYDGSNINVETPPPIL"
BASE COUNT         6150 a         4238 c         4960 g         6220 t
ORIGIN      
        1 attgacagga gcctctataa ccgcactcac gatctatgat atgtgcaaag ctgtcgatag
       61 aagtatgaac tttagggtat ggcttgctac caaatcagga gggcaatcag gtaattttgt
      121 ctctgaacct ccttccgcat aaggcgaccg gtcctgaaga gcaaacatga acaactcaag
      181 gactcggtat catgggctag atgccttgag gggaacggca atggttttgg gaatagtcct
      241 tcatgctgcc cttccataca ttccgggtat cccgtcatcc atttggcctt ctgataatag
      301 ttcttcatac ccgataaaga ttgttttcga attcatccat atgtggcgaa tgccattgtt
      361 tttcatgtta gccggttttt ttgctaattt gattattact cgtaagtcct ggatgttctg
      421 gtgtaataac cggtttttgc gagttgggct acctatcgcg gtattttttc ctgttatgag
      481 cctaacacta ccttggattt ggaaatatgg acaaaccggg gaattttact ttttctactc
      541 gaatacaggt cagccttttc atttgtggtt tttatggcat cttttaatat ttgtcttatt
      601 ttcgactcta tttcgaatgc tcagcaaatc ctttgttgct ttaatgcgcc tgttaaatgg
      661 caacaggttg gaggtattaa cctcatttct ttataaactt aaaaacgtta tagcagtaat
      721 catttttaaa tctaaattcc caatcggatt tatgctcgca tgcggaataa ctaatttatg
      781 ggcaggagga gagttaataa taaatccttt tggtagtggg ttatatttcc tctttggctt
      841 tagcctctat aaaaatccct ctttgttttc tttcataaaa agggaatgga aatactattt
      901 attaatcgct attttcatct tttctttgta tatagcgttc gatatgagag gagtcaaaga
      961 tataacggaa gtgatttatc aaacggggat aggagaaaac ccagctccgg atgtaagtct
     1021 atttgtactg acacggtata gtatggaaac tattggtgcc gtcttgtttt caattggatt
     1081 tatcggtttg gcggaagaca aatttggttc ttatagtcgt ttttcccgat ttatttccga
     1141 tggttcatat tggatgtatc tgattcattt acctgtcgtc gcatttacga cattcttcat
     1201 gtttgggtgg ccaatatacc cagaaattaa atttgtcata gcaacaagtt ttactgctgg
     1261 ggtttgccta gtaacttatc ggtattttgt acgtgctact ttcataggcc tatttttgaa
     1321 cggtaaacga caccggggat cgaattttgg aaatgtgtgt tctgcctgtg gaacgaattt
     1381 acaaaatccg gaacgatttt gtactgaatg tggcatcgaa ctgtctcatt aaaatgtcat
     1441 actttggagc aggaagaagg ggtgctaaac agatggaaat gcatttctgt cgacaaaatt
     1501 gttaacttta taaaaatgct actaatcagg gggtaattta atgaaaataa ccaatataga
     1561 tacgttgata gtggacgctg gctggcgacc ctggaccttt gttaaagttg aaactgacga
     1621 aggtattaca ggttggggag aatgtagtga tggaaaatct ccgcacggga ttgaaggtgt
     1681 tatcagggat ttgaaacctg tgttaattgg caaagacccg tgtgcctttg aaatgagatt
     1741 tcaagaaatg tatataggaa caagagccag taaaggcggg atagctgcca aggccttagc
     1801 agggttggat tgtgctttta ttgatattaa agctaaatca ttaaatatat ctgtggcaga
     1861 gttattcggc ggaccaacta gagataaagt gagagtttat tggtctcact gtggttcgag
     1921 caggatcagg catacggata ttctaggaac tccgcctata gagacttggg atgatgttac
     1981 taggttaggt aaagaagtta aaagcaaagg gtttacagct ctgaaaacca atgtgttgct
     2041 tcccggccag aatgcaacat ttggaggagg ctcttttggt ggggctggaa gtacagatca
     2101 aatagctcca aaatggctaa ttccccatat agaaacgctt attggcactt ttagagatgc
     2161 tgttggtgat gatatagata ttaatttgga tttaaatttt cattttaaaa cagaagcttg
     2221 tttgagaatc gctaaagtgc tcgagaaatt tgacatgtta tggctagaaa tcgatatgta
     2281 tgatcctttg agtttaaggc agattaaaga ttctaccgat actcggattt gtaccggaga
     2341 gaacctgttt tacatgagtg attacctgcc ttattttgaa aatcattccg cggacatgtt
     2401 tatgatcgat gttccgtgga atggcttcag tcagtccaaa aaagttggag acttagccga
     2461 agtatttcag cataatgtgg cccctcacaa ttattatagt catctgtcta catttgttag
     2521 tgcctcacta tgtgcttcat tacccaatgt tcggataatg gaaatagata tcgacgatgt
     2581 tccatggaag gatgatttaa ccacttccac ccccgaagta atagacggat atatgacagt
     2641 accaaataaa cccggctggg gagttgacat caatgaggaa gtagccaggg ctcacccctg
     2701 ggatgagagt aagataatgt ctgttcaatc gacaggctat aaagtgatat aggccaaaga
     2761 taatttggta taaataatgc cagattgaca cccttcattt gtctaaaata gatatctagt
     2821 gggtaagggg ttgccaactg tggtagaatt ttatacttat tgttagcagg ataggcaatt
     2881 ttgcatatcc attagtttag aagcaggaag agaagaagtg aacatatacg ttggaaatat
     2941 ctcgtacggg actggggaag atgatttaag agatttattc gctaggtacg gcgaggtgag
     3001 cgcagttcgt gtcatcactg acagggacac aggtagatcc aaaggatttg gattcgtcga
     3061 aatggccgat gatgatcaag cgaaagaggc tattgcagct ttggatagca atgatttcat
     3121 gggtcgtgat cttagagtta atgaggctcg gcctagagaa cccagatagg cttttgcgaa
     3181 aaccgagagc cttgcaaatc agtaaggctc cccggttacc cggaataccc cgtcgattac
     3241 taattatcta ctgacctaca atgctgtgaa atattgtagg taagttggtg gtttgttcga
     3301 taatagctat tattcaggag atgtgcaggc aaatgaatga tgttaatgct tctgggctaa
     3361 aaactgtggg aacagaacct tactatggta cttttcaagg agagaagtac atccttaagt
     3421 ataaggacgg aaccgaaaaa cccgtagagg aaatgcaggt aaaatcaact ataacttctc
     3481 ctgccgaaaa tgaatctttg aaatgctgtg ctgattatca catcagcggc aacgcatggt
     3541 cgggtcggac agacatagcg aaagtagagg tttctgttga ctccgggaac agttggcatg
     3601 aagccaaact tataggccct tcagaacaat attcatggca gcagtggaat tataaatgga
     3661 ccccaaatac acctggtgaa cacatccttt tatctagagc caccaatagc gatggagagc
     3721 aacagcccct agaatcggtt tggaatgaat tgggctacgc cgtaaacggc tcgaaaccta
     3781 taaaaatagt tgtttcgtct tagctggcaa tcaaattcgc ctatagggag gctcattagt
     3841 tgtcctgggt aaaagaagat cctaaatatg ccgatattgc taatgtcatt aaatgcatgt
     3901 cgataaatga agaagcgatg aatgcggtgt gggatatggg gaataaaatt agttttggct
     3961 cctccgcatt gactagggct caagaagaag taatagcgac cgtagtttcc tctataaatc
     4021 actgtaaata ttgaactgta tcccacggag agtatctccg ccaggcgaca gaggatcccg
     4081 aaatggcatc tattgtgatg caggattatg gtgacgctgt tttagatgat gaaacactgg
     4141 ctatagttga atatgtagaa aagttgacca aagagccttc tgagatgacg gaaaacgata
     4201 tccaaactct tagaaaggca gggttgtcag acaatcagat tctttcgata gtcatgataa
     4261 cggctatgtt tgcttttatg aatagacttg ctgatgggtt gggggtggaa atagaagatg
     4321 agaaaggggc ctttgtaaac tcttggcttc gaacctccaa caactcatcc tcctggttgc
     4381 atcatcaacc aagagaaaga gtgtaaacaa aagaaattac agacctaaat ttgaatcgtg
     4441 tttaagtcga ccttttttga acgaatccac tcgtcaatat cagatcggaa aaatcgccac
     4501 tgatgcccta ctctgttagc cgggatatca ccagccttta ataacttata aatagtcatt
     4561 ctatccagtt gcagataatc tgctaattcc ttggtagtca taagatcgtt gctgaccacg
     4621 gctacacctc cctgaggctt tatactaagt catattaaac taatatgaca tatatattca
     4681 gtatttatgt aactaattag actatttatg ataagaataa ttcatacgtc cgtcaatcca
     4741 tattgaattc atgaattagg tttctgagag cggcgctctt gatgtcgtca aatggccgat
     4801 cgaagagatg aattggagaa tagccatcaa aagaaatact aatcgcattc ccgatataaa
     4861 agtgcttaag attctgcttc cggtctcagc tatcatgacc tcctctacat ttgaagaaaa
     4921 accagcagaa atcattgttc cactaactat agctgtagct atagcgatac ctgtaacgtt
     4981 accggagttt cgggagagat tgactaaagc agatgtaaca ccgtatttat ttccagtggc
     5041 agaagagaag atggcagcgc tattgggaga cataaatatt ccagaccctg agctcataat
     5101 cacaatggct aaaataacga accaaatcga ggtactatca gccataaaag ccatcaataa
     5161 taatccacac cctgcaagta ataatcctaa ggtggtgaat agcttagtac catatttatc
     5221 agataggttg ccacttaatg gacctaagat gatacggctt atagcatttg gaatgagcac
     5281 ccatgaaatt tgtgccgggg ttagccttaa agcggcttga aggtaaaacg taattagaaa
     5341 tcggaaggac gttaccccta agaaagagat gtagctagag ccaattccca ctgaaaatag
     5401 tctactgcgg aataaagaca aatccagcat aggtgaggat gattttaatt cccaccaaat
     5461 aaaagttgta attagtccaa taaaaatcag gaaacctgcc ataataggaa gagagaacca
     5521 tcccaactca gccccatttg ataaggtgag aaggaacaca aggagggagg aagaggataa
     5581 agtcgccccc agccaatcaa agcttttatc tccttgagtc gctccccgaa accttgatga
     5641 atccaataaa agcgccgtac ctaaaaatgt gatggcacac aacggtacat taatccaaaa
     5701 aatccaatgc cagctagcgt aggttactag aaaccctcct actattggac cgattatccc
     5761 ccccgtacca ataatgcttc catgagctcc cagccctttt ccccgttctc ggtctgagaa
     5821 tacagaggtt accattgcca ttccgttggc ttgggtcatt gctgctccaa ctccttgaaa
     5881 aatgcgaaag ggaatcatca acatgatggg gtgagcatac ccgaggtact gggcaatagc
     5941 tggggaactg cccgaaaata gggccattga accaaatagc acaacaccta ataaatacat
     6001 tggttttctg ccgataatat cggataggcg tcccatcggt aataacaacg cactgatggt
     6061 taaggattcg gccagaacga cccattgaat cgtggttaag gatacgtcaa attctctggc
     6121 aattgttggt agagcgatac tgatacttcc atgatgaatc acattagtca gtgagcccaa
     6181 cccaattgcg gcgaaaacca gccatttgta gttggaggaa gagtacgcaa gtttcaacat
     6241 cacattaatt gtactcttag cgtaccgtgc ctgcaccttt gagtatatac tgcgtctgga
     6301 gtaaaaaagg agccaaaaaa catgttaacc acatctatta caggaacacc ctccgaagtg
     6361 ttagtcgagc agctagtagc ttcgggaatt aaatatgttt tctacaattc aggatctaga
     6421 gaggcgttat tttttgatgc tttgaacagc aaccccaata ttgatgggat tttggcactg
     6481 catgaaggct cagtggcttc catggcagga gcatatactc aagtaaatag tgaacccgcc
     6541 gtcatggtgg ttcatttagg tgcagggttg gcccaatgca tggggcagct atataacatt
     6601 tggtttggag gtctacctgt agttgtaatt acctttgctg gagacacagg cagttttaca
     6661 gatagaataa atctagattt ggattcaagt tatagccctg cctcgatttc agtccccatg
     6721 gtaaaggaaa catggacagt aattgaacct gagggccttc ctgcggctgt tgaccgagca
     6781 ttgagggtag caacgactcc accttttggt ccggttcatt tggcagtata tgacaaaatg
     6841 ctggaaaacc aacaggtttc aacagagata attcacggcg gtgccaaaaa cataaaggcg
     6901 ggaaatgcaa cggatcaaga cattgtaaaa atacttgatg ctcttgataa agcagataag
     6961 ccccttctat atgtgggcga tggggtttgg aaaagtggag gcgacaaatt ggctgcagaa
     7021 ctggctactc attttggaac ggtaatcgcc tcaccggaaa ctgacctaag aggtatttcc
     7081 ctcaaacatc cccagcacat tggcatgtca aatacggctt tcgactcata cgagcctgat
     7141 gtaattatct cccttggggt aaggcatcaa ggttcgggga aaaaggaaga ttttgatcct
     7201 ctgagaaaga ccaaacaaat tttggctttg ggatctggtt tggaatattt gaaaaactat
     7261 cccgggttag aaatgggaat cattgctgat gaggcctcct ctatatcgag aatgctagaa
     7321 atagccaaaa acgactacaa ggcagataga tactctaaac gaagagagaa aactttaacc
     7381 ctcgtggaac agcaacgttc taaacttcga gattctttga tgccgtccta tgaaaaaggt
     7441 agggtgcgag ctacccttct agtcgatgca atagatacag aattagaaaa aataggggga
     7501 ggatatatta ccaatgagca atttgctgcg aggtatgatg ccgtgggtcc gggattgtca
     7561 gaacgaaata acattttgat gaaaggggct ggtggatcag aaggatgggg cgtaggagcc
     7621 ggcattggtg caaaattggc ggttggcgaa aagcctgtca ttgggttagt aggtgatgga
     7681 tccctttatt atgccgattc aggactgtgg acagcggtac atcataacgt gccccttctc
     7741 tacgtaatac ctaataacgg agcctatgga atcgtagctg gattctttgg tcagagtggc
     7801 ggcagaatgt cggacacggg taattatcaa ggcgttgttt tagatggtat agaccccgtt
     7861 aagattgcgg aggcatttgg catggaaggt gaaagagttg atgatgaaga taagctgaat
     7921 cagacgatcg aaaaagcgct tgaaatagtc atgaaacaaa acagacctta tttactagat
     7981 attcgccttc ctcttgggtt accggaaggt ggcgtagccg atcagcagta tcggatgaag
     8041 taataaaaga cctgaggtaa atcccaggtc ttttattgtt ttgctttaag tggaggttag
     8101 gtgcccctca gccttggatc caaggcgtcc cttaacccat ctccgaaaag gttgaagcca
     8161 tatacggaaa gggataaacc taccccaggc caaattgcca tccaaggtgc aaggataaaa
     8221 tagtctctcg ctgcgccgga caacatacca ccccatgatg gtctaggtgg cggtactccg
     8281 agtcccaaat aactaagaga ggcttctgtc aatatggcaa tacccaatgc actcgttgct
     8341 attatcaaaa aaggtgcaac acactgtggg gtaatatgct gaaccattat gcggatattg
     8401 ttcgcaccaa tggccctggc ggcatccacg tattgagttt ctttgacggt cagagcctga
     8461 gcccggacaa ccctatttgc tcgtggtatt tgaactactc caacagcgac tattatattt
     8521 tctatggtct gccccaacac cgaagtaaca gccaacgcta aaattaaggt tggaattgac
     8581 attaaggcgt ccatggctct ttgtataatc tgatctgttt ttccgccgaa ccatgcgcta
     8641 atcacaccga gaaccccccc aagccctgat ccaaatgcta cggagagaaa gccgaccatt
     8701 agtgatactt gtgccccaat ggccatctga gcccaaatat ccctgccttt gtcatcggtt
     8761 cccataagga aggttgaaaa tggctccctt tcacttgaac ttaaaggact ctggagttta
     8821 tcaaaaagtc gccattcaat ggggtcatac caggtaatta attccgctgc tgctgccata
     8881 aagcacataa tgataatgat aaaaagccca gctgccgcca aaggacgcct cgctgtaaac
     8941 ttcaaagaag ctgacagtag tcttatcggt aatgaaggag ttgagtccgc aatttgtgct
     9001 aattctgttg acatttagtt ccctatgctt ccgatttatt gatatctcac tcgagggtca
     9061 aaccacccgt atgaaatatc tattagtaag tttacaaata ccacagctat agcaaagaaa
     9121 agggtaattg actgtactat tggaaagtct ctagcaagca ttccctcaag gataaaactt
     9181 ccaagtccag gcattgaaaa aaccctttcc attacaactg agccacccag cgcgagtgca
     9241 gtagttatac cgattactgt tatcacagga atgaatgcgt ttttaagtgc atgccgggcg
     9301 gtaactacgt acgtggtgag accttttgcc tttgctgtgc ggatgtaatc ttgtcttagg
     9361 acctccaaca tggttgatct catcattcgc gcttttgtgg acgcgctgat gtagccaagc
     9421 attatcgatg gccagaagaa ctgttgtatg ttctgccacg gctcttctgt tattgcgaat
     9481 gagcccaatt gtggattcca cataaaaaaa tatgcgccgc ctaataaaag taatgtggca
     9541 ctccagaagg atggtaatga cagaccagct aaagacgcta cacgaatact gtaatccagc
     9601 catgaatctt gcttgagagc cataattatt cccgctggaa gacccaaaat aatggcgatt
     9661 atctgagaaa atatcactag ttcaagtgtt actgggaact ttaatttaaa ttcgtcaaaa
     9721 acatcccttc ctgtaaatag tgagtcaccc caatcaccaa ctacgtatcc ttttatccag
     9781 gtgccatatt gaatatgtaa aggggcatta agtccgagtt tttcacgtat ttcttctaag
     9841 tcttcttccg aaacatcaat tccctcaccg ccggagtttg cttctgcgat cattaaggct
     9901 acatcaccag gggcgattct tagcagggca aaaatcaacg cagaaactgt gaatagggtc
     9961 aatactccaa agaggagtct tcgaatcaaa tattccttca ttttgcctgt tccctgctgt
    10021 ggtgtttctt attcttactc gctgcattat atatatgaaa gggagagtaa gctattttga
    10081 agctatctag ttaagttgtt acttgaaagc ggctcgctaa cccggaaagg tcagcgagcc
    10141 gctttctatt tttttttgtc atcgaagagt gagactgcga gtatatttga ctcggcctca
    10201 cccttcgaaa ctatttatat ggttagtttc tgtctaacca catcctctcc cactgtgccc
    10261 atttattctg ggatgggtga ggatacaatt gccagccttt aacttcattc gaccaggctg
    10321 ggaatatgta ggtccatgga tatggaatag agtacatggc ctctgttgcc aagattctct
    10381 ctatgtcgtg cactagttct acacgttttg ccacatccag ttcactggac tgttcaagag
    10441 ctaatttttc tagcctggct gccaattctg ggtcagcagt ttctcggcct gtgccacggt
    10501 agtaccagct ttctttgtct ttgaccaagt gaatcatgta gaagtcatct gggtcagcaa
    10561 cgttggttct aaccttggaa ccatagtgaa tgtcgtagtc ggcctcttta ccacgagtgc
    10621 tttgtacagc actttctaca gaggcaatag ttacttctac gtcaaggttt ctactcatct
    10681 ggtcctggaa atataagcat ccgtctatgt acatctgtgc actttgtgcc atacattcaa
    10741 cgctgaatct gtttccagct ccaagcactt catctactaa acgatttgcc tctgctatgt
    10801 cagcgacctt tccgtcacct cttctccagc caggcaattc gcctagctcg gaatctggca
    10861 gagcccagat acttcctggt ggcatccaca gggtgtgcct atgcacacct gacacgccag
    10921 cggccatgtt gaaatctacc cagtcatcac ggtcgagcgc taagtgcatt gccattctga
    10981 tcttagcatc gttaaatggc tctctttcca tgttgatctg gatgccttta ccccaagaat
    11041 ggagggctgg cataatcgtg aatttatctg caaatcgtgc ttttatttgc tctacgtgag
    11101 ctggcagcag agatgcgcta ccttcaccat agtaatcagt ctgtcctgtg ataagggcag
    11161 agaatctagc tgtggcgtct gtgatgatga actgatcttg accttcagtc aaaggcagtc
    11221 cctctctcca gtagtcaggg tttgccctga ccttagactg cacgtcaacc tcaaattcag
    11281 taggaacaaa agctcctgtt cctgttccaa acaagaatgt ttcaggtgta gtctcatcga
    11341 gcatgtcgat gtatgcaatt tctaggtcag gatgagtacc ttcctgcctt atataggcaa
    11401 tgaagtcctt gtccatatta ttaggggaac ctagcattcc tgcgaactga ggcagtgttc
    11461 tagctagagc ttccttaaat tcaaccttaa acgtatagtc gtcaacacac gatgatttct
    11521 catagtgacc tagggctgct cttggccctg agtgcaaaat tccttctgga ggatttaaag
    11581 catattccaa gctggcttgt gcgtcctcgc acgtaaagct atccccagat acagctgatg
    11641 cgatttcagc tggaaggttt gggttgactt ggaaattaat gccttccctt agcttgaaaa
    11701 gcacagtgag tccaccgtcc tgaacttccc atgtctcagc aaggtcacct tcaatttcgc
    11761 cacccagtcc cttccaaggg tcatacctca aaagagcgtt gtatgtaggc aggaactgag
    11821 ggccttcaaa gctatgtcca tagtttgggt catgtccact tagcttgtga gaggctccat
    11881 atcgaaaaat tccgcctttt atcggttggc cccattctga gttgtaccct gggccggaca
    11941 tataaagatc catccaggtt tgctccgccg ccttctgtac cggtgtgggt actgcggttg
    12001 ccttagcttc tttagcgacc tctttttgta ccaacgcaac cggtgcagga gctgctggag
    12061 ctgccgctgc tggttgtgca ggagctgctg gagctgccgc tgccggagct gccgctgccg
    12121 ctgccggagc tgccgctgcc ggagccgctg gagcggcggc ctcttgagcc gcacaagcca
    12181 tgaacagtga tagaactgtt atggctccca ccatcatgat taatttactt ctcatcgttt
    12241 tcctcccatc tattaaatgg tttagtttcc tcgttccaaa ttacaacaca gaaaaccgtc
    12301 atcattacac atgacgactg attaaaccat cacatataat gattgactgt ggaggaacaa
    12361 taagggataa actagataag gtcaatctat attcaaatcc taaatcaacc tttagtcgtt
    12421 ttattcggaa tttatatgtg gaatatattt atgccaaaat gtcttaagat agcaaaaaat
    12481 tagtggaatc gctgacaata tgccctggtt gcgggcggag tagtaacccg gagaaaattt
    12541 tttgccctca ttgtaggagc ccattaagta gaggccgtgc agtgccagga gaagaaggcg
    12601 aagcaataga aataaaccga cttcagactt tgaagagagc aaaaaagaag aggtatattt
    12661 tcagaggtgg cttggtcgct gttgtgctga ttattcttgg attactgttt tggaaccctg
    12721 ggaatcaaaa ttttgaacca acaactgata tttctgctca ggtcactggg tttgagtgga
    12781 atatgtatca aggaggtcca gcactttcag gtagcagaga tttaaaccag ttagcgtttc
    12841 ttaatttttg ccttttgttc gattgtgttt ctaattctga ttctaatttc ggggatattg
    12901 tgtggcgtta tcaggccaat ggagctatat cttcttcccc tgcagtcacc gacaaagctg
    12961 tttacattac taccgaagat aggcgaattc ttgcattaga gattgaaact gggaatccga
    13021 tatgggaata ccctgccgag gcacctacag atacttctcc agctgtagca ggtgacatgc
    13081 tttatgttgc cctgagagag ggtaaatttg tcgccctaga ccgaaataat ggaactgtag
    13141 tgtgggattt ccaaagtagt gaaccgtttt tttcgtcccc gacaattcat aatgggcttg
    13201 cttatatagg gtcgggagat aagaagtttt tcgcttttga tgcactgacc ggagagcaaa
    13261 tttgggaata cgacgctggt tcaaggattg ctaatgcagc tgctgttaat gaagatgttg
    13321 ttgctttcac aacccaaaac aatgttgtgc acgttgtgga tgcattgaat ggtgacttaa
    13381 ggctcgatta ccccatcggt tccccgtata gtgcgcctgc aatggatgga aagttgctgt
    13441 tcgttgctga tctgaatggt gtagttaggg ccattgattg gacgaaaatt cagtacccca
    13501 tggaaaaatt agcccgtagg tttagaatga ctatgttttt gtgggggttt gaagacacac
    13561 ctcctgtcat gaaaggattt gtttggaaat ttcgaaaccc gagagaggtt tttcaaaaca
    13621 ccccgacaat tgcaaaaggt atggtctatg ttccctctct taacgggagt atctacaagc
    13681 tcagcgctac aacgggggac atacaatgga ggtataccac cgacggtacg atatcagaat
    13741 cagtctcggt agtcggtgac cttatgctga caggggattc aacaggtaag atccacggtg
    13801 tcgatgtgga aacgggaaag aagatatggg aaatgcaact agacgggcat atttcctcga
    13861 ctccggtagt gtcggcagaa atgttatttg ttgccacaca aaacggatct ctatacgcga
    13921 ttaagtagca tttatgtgag gtgatttaaa ggtggcagat gtaactgcgg accaatttaa
    13981 ctcccacatt gccgaaggta aagacctctc tggacttaat ctttccggcc tcgatctgtc
    14041 taatatgcta atgcagaatt tggttttgcg aggtgctaat tttactggat gcgatctttc
    14101 taggtctgat ctcagtggtt ctgatttaac tagagccaat ttgagaggag caaatctaga
    14161 tgaggcaatt cttcgaaaca ctattttcac aagagctaat ttaactatag ttgacatgcg
    14221 aggcgccgac ttgagatgcg ctaattttga aggagccaac ttaagtggag caccctccaa
    14281 ttcctggcag agcacagcga gagtcatagg tgccaatatg cataaagcta atgctcaaaa
    14341 tgcgaatttc tctaactcaa ttcttgttag agtcaacttt aactcgtcaa atttgtatgg
    14401 agctaatttt gaaggagcaa atataacgag agtatctttg caagatgcta aatatgatat
    14461 tgatgcgttt gatagggcta tcggtgtcta aaattctaaa atttgctgat agaagcaatc
    14521 aaattctctg aatatttatt ggaggtacag actaattgga catagggcat catgatcccc
    14581 aattgctaat tataggtcta ggtaatcctg gtccaaaata ccaacaaact cggcacaatg
    14641 ctggattttg ggtggttgat tttatcgtta atgctctaaa acttaaattg aaaagaagcc
    14701 ataaatccac tcttttggca gaagggtcgc tgcggggttt ggatatagtg gtagcgaagc
    14761 ctaggacata tgtcaattta agtggtgagt ctgcggcgta tctactttct cgctattcac
    14821 ttccaacaca aaaactcctt gtggtttatg acgatatcca tattggtgtg ggaaaaatcc
    14881 gattgagagc aaatggcagc gcaggaggcc ataatggcat gcgatcgata attgattctt
    14941 taaacaccca agattttcct cggttgagaa tcggtgttgg aaaacccgac cttggagaag
    15001 atcaaatcgg ctatgtgtta ggaaaaccta ccttgcttga aaaagagacc attgggaaat
    15061 cagtcgcagg ggcactcgat tgtgtggaga ctttggtaac agaaaatatt aatgttgcca
    15121 tggatcgttt taactgatgt tcgttaggta gattgatgta tttggtgcaa cggcaaaaac
    15181 attatttgac cgttctgttt ctgttagctt ttttagtgtt gagtgctgtc tcgtgtagcc
    15241 aggaagcctt ttcctttgga tccgatgctt tggaaaataa taagagttcc gtggcatcaa
    15301 attttgaaac atgggtgccc ttggtggcaa ttcccccaga tagggatctt gaaaatcttt
    15361 ccacccaata cgggtttggg ccagatgaaa tgttattacc cgtgaaatca gatgataaaa
    15421 acgaaaatgt aggaaaaaag gaggagttta cggtagtcaa tttgtctctt acagagtcct
    15481 atacagtcga aggagagttg gttaatgtta ctgacaatgc ctattggtat gtcgatataa
    15541 acagtgagat tgaccgggat ttgtataaca tagttgcgac gaaatgggag aagatacatt
    15601 ctgattattt tgcaacatac cacgttccga aattgactat tctaaatacg gctttaaatg
    15661 gtgcggcagg atattttagc gacgttgatt cgtatcctag gtgggttcat accaatagca
    15721 acgaacgccc tatggtttat atagatcctt caagaaataa gccaggttcg gacaggtaca
    15781 tgtcggtcct aattcatgag tatcaacatt tcgtcaacaa cgaagcagac cagggggagg
    15841 aagcttgggt agatgaaggt ttggcggagt tagcagtgag aaatataggc tatgaaaccc
    15901 ctttagaaaa gtactttttg gttgatccgg atacccagct taattattgg tcagatgatc
    15961 ctcgcaaaac actagcccat tatggtgctg cttcattatt ttttgagttt gttagcggta
    16021 ggcttggagg ctctcagtcc ttgaggagct tgatagagga accgttagat gggatcttgg
    16081 gagttgagag ttggttagaa aaaaatgaca ctcatttcca tcaagaattt ggacaatgga
    16141 tattggctaa ctatttggga tcagagacag gtcaatattc ttatccgaca cgaactctca
    16201 gattgagatc aggagtagat gaattaaaac ccggcgagaa cacctttcgg gtttctcaat
    16261 atggaactcg atatttcgag attgacgtga acggaagaaa tatagatatt cggttcgatg
    16321 ggagtgattt tgttaatcgt tttaaaaccg aatgccacac gcaatgttgg tggtcaaaca
    16381 agggagattc tatcaactct tctatgacag cgtcacttga tttcacgaaa aagaaagaat
    16441 cgtcactgca ctttgactta tggcacgata tagaagatgg ttgggactac ggctatctat
    16501 ctgtgtcttt ggatgaagga aatacttgga aaactttaaa cattcccggc acaacttcaa
    16561 ccaatccagt gggatcaaat tacggaaatg gctattctgg aagaagcgat tggacgagtc
    16621 atattgttca cttagacgac tatgctggga aaaaaatact cgtgaggttt caatatgtga
    16681 ctgatgctgc cgttcatctc gatgggatgc ttattaaggc agctagattg tctgaaaatg
    16741 gtgagccatc ttcttcagaa cacttaaaat ggattccctc tgggtttgtg ctagttgatg
    16801 atattcttaa acagaaattt ctcgtccaaa ttgtaaaaaa gatgaagtct ggccaatata
    16861 tcattgacgg catcaccttg gatgattcta atgagactct atacacaata cgcgaggatg
    16921 aaaatttaga tagtgttact gttgttgtgt ctgggatgac tggattgact caacaaccgg
    16981 ctgttttcga tctcgaagta tcagaaatct ttgccaaata attatttgct catcgttttg
    17041 taagctctca tgggatgctg caaacgatta gctgcgagtt ctaattgact gtaaataacg
    17101 ataaaaatgt gatttccata aactataaat ggttggtata tgtctccgtt gcagtaggag
    17161 catttttaac ggtctctgat caaacgggga caaatattgc aatgccgagg attgcagaag
    17221 atctctctgc tgacctaccg actgtgcaat gggtatacct tctgtataca ttgtctatta
    17281 gttctttttt gctaccaatg ggccgtttgt ctgacctagt cggaaggaaa agtgtttatt
    17341 gtactgggct ggcaatattt tcaattggag cgctaggggc gtatttatct ggaatattac
    17401 ctattttatt ggcagctaag gcttttcagg gcctgggtgc agctatggtc caggcaaatg
    17461 gcatggctct gatggcagaa tcttttccta gaaacgagag agggaaagct ctcggattat
    17521 atatgacgat tattgggatt ggagctactg gaggtcctgt cattggggga tatttaatag
    17581 gtgcttttgg ctggcgttct atgtatttgg ctatcctcat gtttggggta atggctcttg
    17641 tgctttcctt taaaatactg aaaaaagagg aatttacgtc ccgccccaaa aatttgtctt
    17701 ttgattggat tggagcgact ttatctgcat cggcattagc attgttccta ttgggtctaa
    17761 cctattccta taggttaggc tggagtaacc ccgtcgtcat agtaggcttt ttgctttcga
    17821 ccatatcgtt agctgggttt atatggtggg aaaaacgtaa ccaaaaccca atcgttgatt
    17881 tatctctttt ttccgtaccg gtattttctc ttggaatggc cgcaagatat ttggctttta
    17941 tgtctgggtc ttcggccttt tttcttatgc ctttttattt aatacaagtg gttggactta
    18001 aggaatcaga ggccgcttta gtgttggctc ctagttcgat tatgatggct ataacgagcc
    18061 ctcttagcgg gagattatct gacaaaatag gcactaagtg gccgtcagtg gctggtttgg
    18121 tttgttgggc ggcggcggtg gctgtttaca ccacattaac tgtcgattct tcgccttgga
    18181 ttgtagttat agggatgttt ttacagggtt ctggaaatgg gatatttggt tcaccaaata
    18241 caactgcggt catgaatgtg gtggaatcaa acagatatgg tgttgtttct gccttgttga
    18301 acatggttag gacatccggt aacttgaccg gaatagctat gggcactact ttggtggttt
    18361 tcaccatggc ttcaatgaac tacccgccag atttgtcttc tattgaatca gttggagcca
    18421 catcctatgg agagggtctg aaaatcgcat tcaccgaagg cataactagg gctttttgga
    18481 ttggtacagg aatcgttgga tttgccgcac tttttacggc ttttagaccc gaaaaaccaa
    18541 ttaaacaaac gaaaactacc ggtacgcgtt aagtcgctgt tatactttgt tcccaatagc
    18601 aaaccatacg gtataaatgt aagtaataag actggaaatg gggttgatat atgagtggat
    18661 ctgattactc tttctcgaca ttttcaaaaa acgaattcta ctcccgatta aatgctcgat
    18721 tagtggatat ggcggaaatt ggatctgatc ttagagttgt agacctggct tgtggaactg
    18781 gtggtgtaac ccgtcttatt ttggaaagaa taaaccgtac acgagataca gtagttatag
    18841 cgctggatca ttcgcaaacg gcattaaaag ctgcaatgga agatctcaaa gatatttctg
    18901 ataatgcggt taaatacgtc cagagtggtg ttgaaaatgt gtcagaagcg gtgaaaggtt
    18961 cggtagacac cattgtcttt tgcaatgcta ttcattacct tcctgataaa gataaagtag
    19021 ttcatgacat tggcactgcc ttacgtccgg gagggaaatt cgcattcaat acctcttttt
    19081 atgagggtgg gcagcatgaa gaatcctcag ccttttatag taaatggatg ttcaaggctt
    19141 ttcgaatcct taggaaggag tatgggttga aacccaacag agcggcaaaa gtggaatcaa
    19201 ggcggcaact gacccctgag gaatacagtg atctcttgga aaggaatggg ctcacgatac
    19261 tcaagcaaaa ggtcgataca gtacaggttc cgatagaagg ctggttggat ataagtacct
    19321 tttcagattt catagaaggt acaatgccag gtgtcccgat gaatgaggct agcgccgcat
    19381 tgcaggaagg aataagacag acttactccg aacttaatgt tacccacgtt cctagaaatt
    19441 ggctcgacat cattgctgtt aaacgctgag atacaatatt cctatatacc cttttcctat
    19501 caataccaag ttacaataac tcgcacgtaa ttccacaatg actgactcat aatcaggaaa
    19561 ataataggaa actatttcat attatctttt atgtaatcac atgctttttc catgtaatct
    19621 ccacacctaa acccgctgtt gttgataacc acttcctcaa atgcaaagga tttcgtccct
    19681 agccatctct atccagcgag tatactgctc acccaccttt gaataagagg ttagcttttt
    19741 gacatgctaa ttaagttgcc tagattgctt acttatcaaa taacttaatc tataattggt
    19801 gttcgttaca tttttggttc actcaatata gcgagagtat ttatgacatt gataaatgag
    19861 cttcccgacc agtccggcag atatggtcag tttggcggca gatatgtccc tgaagtttta
    19921 atgccagcta taaacgaatt ggaagaatcc tatcgtcaag ttcaaagtga caataaattc
    19981 cagacagaac tttcccaatt attgataaat tttgttggta ggcctactga gctctatttt
    20041 gcctcaaacc ttactgagag atttggaggt gccaaaattt ttcttaaaag ggaagattta
    20101 gcacatacag gtgcacataa aatcaataat gctttgggtc aaggtctttt agctaggaaa
    20161 atgggtaaaa ccaggataat agctgaaaca ggagcagggc aacacggagt ggcaacggct
    20221 acggtgtgtg caaaattggg attagattgt gttgtttata tgggtgaaga ggacataaga
    20281 aggcaggcat taaacgtttt ccgaatgaag ttgttaggag ctgaagttat ttctgtggat
    20341 tctggtagca aaacccttaa agatgctatt aatgaagcga tgagagattg ggtaacaaat
    20401 gtcaatgact catattacct aataggaagt gttgttggtc ctcatcctta tccaatgata
    20461 gtgcgggact ttcaaaaaat catagggtta gaaactagaa agcaaatatt agaacagatg
    20521 ggaaaacttc ctgattatct aatagcctgt gttggtggtg ggagtaacgc tatgggatta
    20581 ttttatgatt tcatcggcga taaggaagtg aggctggtgg gggtggaggc aggaggagaa
    20641 ggtattgaat ctggtagaca ctcttcgaca atttcagcag gatctatagg ggtgttacac
    20701 gggagcatgt cctatctgat acaaaacgag gatgggcaga ttatagaaac ccacagtata
    20761 tcggcagggt tagattatcc aggcgtgggc cccgaacata gttggctcaa tgatagtgaa
    20821 agggcatctt acgtcagtgt ggatgattcg caagctttgg aaggatttaa actgatgtgt
    20881 gaattagaag gaataattcc agctttggaa cccgcgcatg ccatatacta tgtatctcag
    20941 ttggcaccta ccttgaagaa agagcagatt attgtaatag ggctatctgg tcgtggtgac
    21001 aaagatatgg aaacggtagc ctcggaaatg aatgttcagg tgtaaggcaa catgaaatat
    21061 tttgccgcca ggttatccca tgccatttta tggagaattc cgcctgtgct tttacttata
    21121 ttctttttgg gcccttcaat aggggtgatg ttcaaccatc attttcccga acaaaatcct
    21181 taccatcccc attttgccga cggcttaaag cattcccatt ccgttttggg cgaacataca
    21241 catgaaaagg caaatgcctc accttcctat aatgtagctc ttttggtcaa gaatgttggt
    21301 ataagtacat cagggattca tattgctctc atttgttttg agactcaggt accatcgccc
    21361 ccattttcct tggccaaatc attgattttg accatggata tctacgatgg gagcaacatt
    21421 aatgtagaga cgcctccacc aattctatag cgacgctata gaaattcggt gtatttaccg
    21481 attcgaaggt ggaggtaaaa atgctctggt tccgcgctgc tatatttgtg tcgcttctat
    21541 tcttaggttt gtataaccaa tcaggcac
//
LOCUS       DASCNS010000174         6573 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001391851, whole genome shotgun sequence.
ACCESSION   DASCNS010000174 DASCNS010000000
VERSION     DASCNS010000174.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001391851"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(223..1971)
                     /gene="ggt"
                     /locus_tag="QF838_06315"
     CDS             complement(223..1971)
                     /gene="ggt"
                     /locus_tag="QF838_06315"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJO60329.1"
                     /translation="MSLYSAWKVNKEEVVAENGIVTSALPLSAEAGLSILKSGGNAID
                     AAVAIGFCNIVQEPYMASIGGMGYMLIHLAEEGRTLGIDFNGRAPKGTTDDMYEVLGP
                     SIGAGYKTFDVVNDANNTGPLSATVPATCAGFCEAHRRFGTLPFEQVLESAIHLASHG
                     FEANWYLTMSAANMYDTFNSDSHLSSIWLPGGKVPTSHPKPGTKIIQKDLADLLKQLA
                     VHGRDAMYSGEVADAIDEHMKKNGGVLSKQDLSDYHPSIEEPAQTTFQGHSVNVVRTP
                     SGGITNLQTLNILDQFDLKAMGHNSVDYLHTFMMAARHAFADRFGYLGDWEHSKVPLN
                     GLLSAGYAKEIAKEVKRPTFEIRNLGEREPLVQFMENPIHNPWDHEKSRKVPVPSHPA
                     MDTNNEDTTHINVVDKNRNAVSCTHTGVFAKGLNPPGTGVALVGGMGWFIPKPGYPNS
                     MAPWKRPMNNMSPFMVLKNNRPVLLEGAPGARRIMNRNAQVVLNVILFGMTPQEAIVA
                     PVVDVSGSKTYVDSRMPESTIKALVSLGHDIELLEEEPAPTGYFARPSAIQVDYEKNL
                     LRAGVDSFRPTTALGY"
     gene            complement(1982..2899)
                     /locus_tag="QF838_06320"
     CDS             complement(1982..2899)
                     /locus_tag="QF838_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO60330.1"
                     /translation="MAVDYLKRDGIGYVTINNPAKANILDRQTSNDISEIWKDMWEDP
                     DVRAVILTGVGDKHFCAGHNLDSRPDITAEERERIRAENIFWPLAGTVNGARIGADGR
                     LGDHYPQIWKPVIGAINGWAAGAGFYLMLTSTDIRLASRENARFKFALLTQGWVGSGP
                     GATYLTRQVSHADAMKILLTDEPFDANEALRMNLINEVVPHAELITRAEEIASGIVKM
                     PPLAVRMMKEFVIRFGDIPITEAWRVQTLMNALLTQLSADGEEGRRAFLEKRPPEFTG
                     SILQKGEAYESLTDEERARLDEIRREQLG"
     gene            complement(2913..4085)
                     /locus_tag="QF838_06325"
     CDS             complement(2913..4085)
                     /locus_tag="QF838_06325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60331.1"
                     /translation="MGNFPLNGLRILDFSWIIAGPTAARHLALMGAEVIKIGSSRRPD
                     PSSRGAAFQVYNQSKEYCSLNLSTPQGLEIAKELVTTCDVVIENFAAGVFERIGLDYE
                     TLIALRPDVILVSSAGTGHTGPDKDYVAYGSLLQHYTGWNTISGYGKDEPIRGGLWAD
                     PWVGMELAMITVAAINHRMKTGKGQYIDFSMAESLTASIPAGLLEYQITNQLPTPIGN
                     DDVIKSPHGLYKCKGYDAWIAISIQNEVQWGSLCKLLDIPKILNSSNCSSPKARQENK
                     VEIDQEINKFTSSLGNYEAMDIFQNHGIPSGPSLNISEAYSDPHNTHSGYLAPQIYPD
                     GATRLMPGMPWKTEESEPVHVIPAPQIGDSNHRIYMELMGMSSDAYQECIRNQIIY"
     gene            complement(4087..5292)
                     /locus_tag="QF838_06330"
     CDS             complement(4087..5292)
                     /locus_tag="QF838_06330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60332.1"
                     /translation="MTDSQDTSILDGLRVLEIGLGVSAPYAALNFAKLGAEVVKLEPL
                     EGDPSRTHGPFPNGIKHKEKSGLFLAYNHGKKSICLDLNISQDRDKFESMLPHFDLVI
                     ENQNLGFFNDRRLGYKKISSINPSLIWVSILPFGEVGPYSQYVADDLVLFHMSGNAHG
                     MIGPVDNPEVEPPIRAGGYQAEMVAGLTASTAAMVAMHQRLKTGKGCKIVVSGLDSMI
                     TMAISGIANSAFGQQPTTRELSKEKGSSIGGMVSAIGGVLKCNDGYVAISPREDSQWE
                     RWLELMGNPRWADDERFATRENRQTNVSALWELLTEWSLPRSKFDIARSGQEKRIPCF
                     PVNTIEDLLADEHLAYREFFVEIAHFDAGILLHPGLPYKLNNTNIPQGISSAPRLGSH
                     TQEILERCT"
     gene            complement(5302..>6573)
                     /locus_tag="QF838_06335"
     CDS             complement(5302..>6573)
                     /locus_tag="QF838_06335"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJO60333.1"
                     /translation="LADTVDRTSDEWRSTFELFSREGKILEEGEILKQPDLARTFERL
                     IEVERANKDKGREKAIQAARDFFYKGDIAKEIIEYSDKLGGNLSLSDLSDFHVEIEDP
                     TTARYKDYDILTCGPWSQGPVTAQVLQMLEDDDISVIPQNTADYIHLLSQAFNLSFSD
                     RHEFYGDPDFVDVPINGLLSKEYTKTQRTRISMETAFSEMPDPGNPWAFEDRIGREKL
                     GHPDINRDRLEQDTSYTCVVDKWGNSFSATPSDAVFGSPIVPGLGLMISSRGTQSWLD
                     PEHPSSVEPWKRPRLTPNPAMAFKEGKLFMPFGTPGGDAQCPAMVQTFLNIVEFGMNP
                     QAAIEAPRFVPWNFPNSFWPHAYHPGLIHLEGRISREVGQNLSSRGHHVEYIDDWSPS
                     TGAMSCIVVDQDTGALSAGADPRRDAYAIGR"
BASE COUNT         1692 a         1533 c         1386 g         1962 t
ORIGIN      
        1 tttaagacca tgagatcatt cttagcggag gcatatccgg tgtaatggct gaaaaatgct
       61 aatgcgaaga cgatagaaat gaaaccgtaa gctgccaatc gtaatttact cataaatctc
      121 cacttggaat gatttatcgt tcggaatatt tacaattaaa tctattttag ttggtcttag
      181 acctgaacgc tattattgaa ctagatgtct agtttttatg tttcaatatc ccagagcggt
      241 agttggtctg aaggaatcta ccccagccct gagtagattc ttttcgtaat ccacttgaat
      301 cgctgatggt ctagcgaaat accccgtggg tgcaggctct tcctccaaca gctcaatatc
      361 gtgaccaaga ctaacaaggg cttttatcgt ggattcaggc attcgactat ctacataggt
      421 tttgctgcca ctgacatcaa cgacaggtgc tactattgcc tcttgtgggg tcataccaaa
      481 cagaatgacg ttcaagacaa cttgagcatt tctattcatg attctcctag ctcccggtgc
      541 gccttccaaa agaactgggc ggttgttttt aagaaccata aatggggaca tattattcat
      601 gggacgtttc catggtgcca tggaattggg gtagcctggc ttaggtatga accaacccat
      661 cccaccgacc agagcaacac cggttccagg cgggtttaat cctttggcaa aaactcctgt
      721 gtgagtgcaa gagacggcgt tcctgttttt gtccactacg tttatatgag tagtgtcctc
      781 gttattagta tccatggccg gatgtgaggg taccggtacc tttcgcgatt tctcatggtc
      841 ccagggattg tggataggat tttccataaa ctgaaccaat ggttcacgtt caccaagatt
      901 tcttatttcg aatgtcggcc tcttgacctc tttggctatt tctttcgcat atccagctga
      961 aagtaatccg tttagtggca ctttggaatg ttcccagtcc ccaagatatc caaacctgtc
     1021 agcaaaggcg tgtcttgcag ccatcatgaa agtatgcagg taatcgacac tattgtggcc
     1081 catagccttt agatcaaatt ggtctagtat attcagagtc tggaggttgg tgataccacc
     1141 gctcggagtt ctaaccacat taacactatg accttgaaat gtagtttgag caggttcctc
     1201 gattgaagga tggtaatctg ataaatcctg cttgctcagc actccgccat tttttttcat
     1261 atgttcgtct attgcgtctg ctacttcccc tgagtacatg gcatctcttc cgtggacagc
     1321 caattgtttt agtaaatctg ccaaatcttt ttgaataatc ttggttcctg gttttggatg
     1381 acttgtaggt accttgccac ctggaagcca aatagaggac agatggctgt cactattgaa
     1441 ggtgtcatac atgttagcgg ccgacattgt caggtaccaa ttagcctcaa aaccgtggga
     1501 tgcgagatgt atagcagatt ccaatacttg ttcaaacggg agtgttccaa atcttctatg
     1561 tgcctcacag aaccctgcac atgtggctgg tacggtggct gaaagaggtc cggtgttatt
     1621 cgcatcattc actacgtcaa atgttttgta cccggcgcca atagagggcc caaggacttc
     1681 gtacatgtca tccgtagtgc ctttaggggc ccttccatta aaatcgattc caagggttct
     1741 accttcttct gctaggtgaa ttaacatgta acccattccc cctatgctag ccatgtaagg
     1801 ttcttggact atgttgcaaa atccgatagc tactgccgca tcaatggcat tgccacccga
     1861 ttttagaatt gaaagccctg cttcagcaga taaagggaga gccgatgtca ctatgccgtt
     1921 ttctgccacg acttcctctt tgtttacctt ccaagcacta tataaggaca tatcgactcc
     1981 tttaacctaa ttgttctctg cggatttcat ctaaccttgc tctctcttcg tcggttagtg
     2041 attcatatgc ctctcctttc tgcaaaatag aaccggtgaa ttcaggcggc cgtttctcca
     2101 agaaagctcg cctaccttcc tcaccatcag cactaagttg agtcaacaaa gcattcatta
     2161 aagtttgaac cctccaggcc tcagttattg gtatatcccc aaacctgatc acgaattctt
     2221 tcatcatcct aacggctagg gggggcattt tgactattcc agaggcaatt tcctcagcgc
     2281 gagttattaa ttcagcatgc ggaaccacct cgtttataag gttcattctt aaagcttcat
     2341 tggcatcaaa aggttcatca gtcaacagga ttttcatagc atcggcgtga gagacttgtc
     2401 ttgttagata tgtagcccct ggcccgcttc ctacccaacc ttgggttaat aaagcgaatt
     2461 taaaccttgc gttttcacga gaggcaagcc ttatatctgt tgatgtcagc attaaataga
     2521 atccggcccc agctgcccaa ccatttattg caccaataac aggtttccaa atctgagggt
     2581 agtggtctcc cagcctacca tctgcaccga ttcttgcacc attgacagtc ccggccaaag
     2641 gccaaaatat attttcagct cttattcttt ccctttcctc tgctgttatg tctgggcgag
     2701 aatctaaatt gtgtccggca caaaaatgtt tatctccgac cccagtcaaa ataacggctc
     2761 ggacatcggg gtcttcccac atatccttcc aaatttcgct aatgtcattc gaggtttgcc
     2821 tatccaaaat attggctttt gccgggttat ttattgttac gtacccgatt ccgtcccttt
     2881 ttaaataatc aactgccatt attcacctct tattaataga ttatttggtt tcgtatacat
     2941 tcctgataag catcggaaga cattcccatc aattccatat atattctatg gttactgtct
     3001 ccgatttgtg gggctgggat tacatgtaca ggttcgctct cttcagtctt ccaaggcatc
     3061 cctggcataa gtcgggtagc gccatcagga taaatttgcg gggccaaata acctgaatga
     3121 gtgttatgag ggtcggaata ggcttccgaa atgtttagag agggtcctga tggaatccca
     3181 tggttctgaa agatatccat cgcctcataa ttacctaaag aggatgtaaa tttgtttatc
     3241 tcttgatcta tctctacttt gttttcctgt ctagccttcg gggatgaaca attcgagctg
     3301 ttaaggattt ttgggatgtc taacaattta cataaacttc cccactgtac ttcattttga
     3361 atgctaatcg cgatccatgc atcgtaaccc ttacatttat ataagccatg gggagattta
     3421 ataacatcat cgttaccaat gggtgtgggt aattgatttg ttatttggta ctcgagtagg
     3481 cctgcgggta tggaagcagt cagagattca gccatggaaa aatctatgta ttggcctttt
     3541 cctgtcttca tcctatgatt aatggcagca acagtgatca tggctaattc cataccgacc
     3601 catggatccg cccagagtcc gcctctgatt ggctcatcct ttccataacc ggatattgtg
     3661 ttccaccctg tgtagtgttg tagcaaacta ccataggcta cgtaatcttt atcaggacca
     3721 gtgtgccctg tccctgcgga agagactaat ataacgtcag gcctaagggc tattagagtt
     3781 tcataatcca atccaattcg ttcaaataca ccagccgcga aattttctat gacaacatcg
     3841 caagtggtga ccaactcctt ggcaatttcc aagccttggg gggtagataa attcaaggag
     3901 cagtattctt ttgattggtt ataaacctgg aaagcagccc cacgcgagga cggatctggt
     3961 cttcgtgaac taccgatttt tataacttcc gcacccatta aagcaaggtg gcgagcggca
     4021 gtaggacctg cgattatcca gctaaaatct agaattctta agccatttaa aggaaaatta
     4081 cccattttag gtacaccttt ctaggatttc ttgggtgtgt gaaccgagtc tgggggcaga
     4141 ggatatacct tgtggaatat tggtgttatt aagtttgtag ggcaatcctg gatgtagcaa
     4201 aattccagca tcaaaatgag ctatctcgac aaaaaattcc cggtaagcta aatgttcatc
     4261 tgccaataaa tcctcaatag tatttacggg aaaacaaggt atcctttttt cctgtccgga
     4321 tctagcaata tcgaatttag atcttggaag cgaccattcc gtaagtagct cccatagtgc
     4381 tgaaacgttg gtttggcgat tctctcgagt ggcaaacctt tcgtcatccg cccaccgtgg
     4441 attgcccatc aattccaacc atctttccca ttgagaatct tctcgggggg agattgccac
     4501 gtaaccatcg ttacatttta atactcctcc gattgccgac accattccgc ctatagagga
     4561 gcccttttct tttgaaagtt cacgagtcgt tggctgttgg ccgaaggcag aattcgcgat
     4621 tccactgata gccatggtta tcatggaatc taaacctgaa acaacgattt tgcatccctt
     4681 tcctgttttt aatctttgat gcattgccac cattgcggcc gtagatgcag taagaccagc
     4741 aaccatttca gcttgatatc cacctgccct tataggcggt tcaacctcgg gattatccac
     4801 tgggcctatc attccatgcg cgttgccgct catatggaaa agaaccaagt cgtcggcgac
     4861 atattgcgag tatggtccaa cctcaccaaa gggcaatata ctgacccaaa ttaaggaagg
     4921 attaatggaa cttatttttt tatagcctaa tcttcgatca ttaaaaaacc ctagattttg
     4981 attttcaatt acaaggtcaa agtgaggaag catcgactca aacttatctc gatcttgtga
     5041 aatatttaaa tctaggcaga tgcttttttt accgtggttg tatgctaaaa acagaccact
     5101 tttttcctta tgtttaatac cattagggaa tggtccatgg gtccttgatg gatctccttc
     5161 tagaggctct aatttgacaa cctccgcacc taatttggca aaatttaggg ctgcatacgg
     5221 agcagaaacc ccgaggccta tctctaggac ccttagacca tccaaaatac ttgtgtcttg
     5281 tgagtcagtc acagatttgt actatctacc tatcgcgtaa gcgtcccttc ttggatccgc
     5341 ccctgcactt aatgccccgg tgtcttggtc cacgactata caggacatag ccccagtgct
     5401 gggcgaccaa tcatcaatat attccacatg atgaccgcgg ctggacaggt tttgtccgac
     5461 ttctcttgat attctacctt ccaaatgaat caacccggga tgataggcat gaggccaaaa
     5521 agaattgggg aagttccagg gaacgaaacg aggcgcttcg atagcggctt gtggattcat
     5581 cccaaattca acaatattta ggaatgtctg taccatggct gggcattgtg catcgccccc
     5641 aggggtgcca aagggcatga aaagttttcc ttccttgaaa gccatcgcag gattaggagt
     5701 taaccgaggc cgtttccaag gttcaacgga agaggggtgt tccgggtcta gccaactttg
     5761 tgttcctcga gaagatatca tcaggcccag cccggggacg ataggagaac caaaaaccgc
     5821 atcacttggg gtcgccgaga aggagttacc ccatttgtcg actacacatg tataagaggt
     5881 gtcttgttcc aatctatctc tgttgatatc tggatgtcct aatttttctc tcccgattct
     5941 gtcttcaaaa gcccatgggt taccagggtc gggcatttca gaaaatgcag tttccatcga
     6001 gattcgggtt ctttgagttt ttgtgtattc tttggacaat aggccgttga tgggtacgtc
     6061 aacaaaatca ggatcgccat agaattcatg acggtctgag aaagacagat taaaggcctg
     6121 tgataggaga tgtatatagt ccgcagtatt ctgaggtata acggatatat cgtcatcctc
     6181 tagcatttgt aatacttggg cagtgacagg gccttgtgac catgggccac aagtcaaaat
     6241 atcataatcc ttgtatctgg ctgttgtagg gtcttctatt tctacatgaa aatcagacag
     6301 atcggaaagg gataaattac cccccaattt gtcactgtat tctattatct cttttgctat
     6361 atcacccttg tagaaaaagt cccttgccgc ctgtatggct ttttctctac ccttgtcttt
     6421 gttggcccgt tccacctcta ttaaccgttc gaaagtcctg gccaagtcag gttgttttaa
     6481 aatctccccc tcttcaagaa tcttaccttc acgtgagaac aattcaaaag tggatctcca
     6541 ttcatcggaa gttcgatcaa cagtgtcagc aag
//
LOCUS       DASCNS010000175         2343 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001392619, whole genome shotgun sequence.
ACCESSION   DASCNS010000175 DASCNS010000000
VERSION     DASCNS010000175.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001392619"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="QF838_06340"
     CDS             <1..519
                     /locus_tag="QF838_06340"
                     /inference="COORDINATES: protein motif:HMM:NF039905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60334.1"
                     /translation="VAQIQMNHLLTYSINLIGWGLVTDVGKRSEAFRWLGPSRYTLSS
                     IIEIFLRKTRKARLVIDQKTIVGQFSFIAACNSVHVGKGMKMAPSAELSDGLIDLLTV
                     EGDITRRRLLSVLPKLFDGTHVNEPEVTYYQASSFSLYPETDDTLNIDGELLGTTPID
                     VSVLKHAIEIFA"
     gene            complement(768..1664)
                     /locus_tag="QF838_06345"
     CDS             complement(768..1664)
                     /locus_tag="QF838_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HJO60335.1"
                     /translation="MVYRYFVCDVFTKQMFRGNQLAVLPEAQGLSESQMQNIAKEFNF
                     SETTFVFPPEAGQTKMVRIYTPNSEVPFAGHPNIGTAFILAKNGVFGTLDTGKEVVFE
                     EKAGLVPIKIKRDESGYIWCELSAPEVLSIRDPVSKSLVASVLTLNESEILTTTHPPQ
                     VASVGLPFLLVELSSVNSLVRAQIDISKLKLFFEKAGVSYIHLYCRNVEDFDIKARMF
                     APLDGVSEDPATGSANCALIGLLSQYENIDDFDKNWTISQGAEIGRPSVLYGRTQKQK
                     GKVVGVWIGGHSVLVSEGTLKL"
     gene            2163..>2343
                     /locus_tag="QF838_06350"
     CDS             2163..>2343
                     /locus_tag="QF838_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HJO60336.1"
                     /translation="MAQSTDFEVNISLQKKLDYDAIVIGAGISGLYQLYKLRELGLKV
                     RVFETGTGVGGTWYWN"
BASE COUNT          704 a          487 c          475 g          677 t
ORIGIN      
        1 gtggcccaga ttcagatgaa tcaccttctc acctattcga ttaacctgat tggttgggga
       61 cttgtaacgg atgttgggaa aagatcagaa gcttttcgtt ggctgggccc aagtcgctat
      121 accctttcca gcattattga aatatttttg cgaaaaaccc ggaaagctag attagtcata
      181 gatcaaaaaa caatagtagg acaattttct ttcatagcgg catgtaactc cgtccacgta
      241 gggaaaggaa tgaaaatggc accgtctgca gagttaagtg acggacttat agatctcctt
      301 actgtagagg gagacataac tcggcgaagg ctcctttcag tgctgcctaa gctttttgac
      361 ggcacacacg tcaatgaacc ggaggttacc tactaccaag cttccagttt ttcactctat
      421 ccggaaacag acgacacctt aaacatcgat ggagaattgc tgggcaccac tccgatagat
      481 gttagcgtac tgaaacatgc aatcgagata tttgcgtaaa tctgaaacac taaaattgaa
      541 atagatgtaa tagtcctcac tttactgtca atgactaatg ctatttgttg tttccaactc
      601 attttcgagg ccattcataa tgatcacatt aatacatagt tcccattgga atacctactg
      661 aacttaagac cctgaaatgg cttttagtaa tgattcagtg agttctggtt gagaatagca
      721 tcatagaaat cggaggaaat gggtgaagaa tgttggcaaa tgaaattcta gagcttcagg
      781 gtcccttcac ttacgagaac actatggcca ccgatccaaa ccccaacgac ttttcctttt
      841 tgcttttggg tcctgccgta taaaacgcta gggcgcccaa tttctgcgcc ctgcgatatt
      901 gtccaatttt tatcgaaatc atcaatattt tcatattggc ttaacaaacc gataagagca
      961 caattcgcac tacctgtagc tggatcttca gacaccccat ctaggggggc aaacatcctt
     1021 gcttttatgt cgaaatcctc gacgttccta caatataaat ggatatatga aactcctgct
     1081 ttttcaaaaa ataatttgag tttactaata tctatttgag ctctcaccaa ggagttcacg
     1141 gatgaaagtt caaccaatag gaaaggaaga cctaccgagg ccacctgggg aggatgagtg
     1201 gtggtaagaa tttcggactc attcagcgtc aggacagagg ctacaagaga tttagaaacc
     1261 ggatctctga tagataaaac ttctggggcc gaaagttcac accaaatata tcctgactca
     1321 tcccttttga tttttatggg gactaaccct gctttttctt cgaagaccac ttctttgccc
     1381 gtatctaaag ttccgaaaac tccatttttt gccaatataa atgctgtccc aatatttggg
     1441 tggccagcaa aaggaacttc cgaatttggg gtatagatac gaaccatttt cgtctgcccg
     1501 gcttctgggg gaaagacgaa tgtggtttca gaaaaattaa attccttagc tatattctgc
     1561 atttgagatt ctgacaaacc ctgagcctcg ggtaacacag ctaattgatt ccctctaaac
     1621 atttgcttag taaagacatc acaaacaaaa taccgatata ccatgatttc tcgaatccct
     1681 taactggttt ttctatctcc aattatccca tctattgaaa atttgggctt ccggatataa
     1741 aacacagtca gtccattaaa agactccctt agttgtataa gtttttggta gaccataaag
     1801 ttgctctcag caatcaaaca tcaaagatgt ataaattgca gtcaagtcca gttagagcta
     1861 tttccagagt tttcccaggg cgggactcta gtaataagga agattcacct ttgcaggatt
     1921 gataaaattc accagtgttt gaatgtgctg ataaaagggt accaaataga acttagccgg
     1981 gcaggatagt atttcggcac cctgctttgt attctgtttt cggatatttc caggaacaac
     2041 tgtagggata cgctcctgat atatattctc acagctctaa cctcacacta gcagataaat
     2101 tgttgaacta cctttctttg gagaggtata ttaaggaaaa tgaatcctag gatagaggct
     2161 ccatggcaca atctacagac tttgaagtaa atatttcgct tcagaaaaag ctggattacg
     2221 atgccatcgt gattggtgct ggtatttctg gcctttacca actatacaaa ttgagggaac
     2281 tagggttaaa agtccgagtt tttgaaacag gtacaggggt tggaggaact tggtattgga
     2341 aca
//
LOCUS       DASCNS010000176         6884 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001393496, whole genome shotgun sequence.
ACCESSION   DASCNS010000176 DASCNS010000000
VERSION     DASCNS010000176.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001393496"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(174..2762)
                     /locus_tag="QF838_06355"
     CDS             complement(174..2762)
                     /locus_tag="QF838_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013727.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HJO60337.1"
                     /translation="MNENIDGTQPNIIPFNPSIILFWVAIAAVCASSIIATAFFESLI
                     PGFLTGYISSFVAWTGLGIILPCLWLIALGIVSGLHKLKHVGSSYPKEIVGSLVITGS
                     LWGAISFIHFTDVETISNIGRGSIGGDVGLHIIGGNALMGWLRIFLLFYAGLVIIKPP
                     ISVKSWQIIILTIWSLGLASKFVYLNIFRKKSTENIPQEIGSKPFVPIPPEVLTPHMK
                     ESHIIKNASAAKKPSTENSLIEIANKEPILADSSPTTTDLLSSYISSSNVTVYQLNET
                     EEESTATVNETVLESIQSEPDLINNGNTTSESNKFNKYWSKSDPRETQSVEIDSQTTN
                     LTNPEQNLDGEIDQDLKTPLPNPASVSTSWKLPDMKLLSGISEGGISKEEIRDTADII
                     KNTFADYKIEIAVEKVRPGPTVTMYGIQPGWVRKVKRVKSRDKDGNLEIDTNGKPVMV
                     QQEEKTRVSVDNILRREKDLSLALKTPSLRIETPVMGESLLGIEVPNHTPSLVALKNV
                     MESPPYTSLAKKSQLPVALGKGSDGEEVSFDLSKMPHLLIAGATGSGKSVCINAIISG
                     LIMEKTPSEMQMLLIDPKRVELTPYNGIPHLMTPVIVETDKVVGILKGLIQEMMNRYR
                     RMEETGVRNIASFNSKGGAKMPYIVVAIDELADLMMTAAFDIEQSICRLAQLGRATGI
                     HLIVATQRPSVDVITGLIKANFPSRISFAVTSQIDSRTILDTMGAERLLGRGDMLYQP
                     IDATRPARVQNVFIGDDEIGDIVQHWSSTPRGYKPEINLNIVNDENSDIAGMSDTGGA
                     NNQDELFEKAMALAQKYTKMSTSLLQRRLRIGYPRAARLMDELEDMGVVGPSDGSKSR
                     DVISST"
     gene            complement(2866..4215)
                     /locus_tag="QF838_06360"
     CDS             complement(2866..4215)
                     /locus_tag="QF838_06360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HJO60338.1"
                     /translation="MITSNNKNLKIIPLGGLGEIGKNMMAIEYDGQILIIDAGIAFPS
                     EIKPVSSFGVSDTSYLNDRKNMILGVLITHGHDDHIGGLPHFLNEYNVPVYSSRLTRE
                     FIKFKLGNKNKVDLRTINFYESYSLGCFEFEFFPVCHSIPDAAGITIKSPVGSLVHTG
                     DFKFDDNPTLGNPVDLEYLSNLSSNTNLILCSDSTYAEKAGFTDSEATLEEELNKIIS
                     QSEGRVLIATFSSLLSRIQQIITISSAIGRKVSVVGRSMNRNLKVALDLGYVTDPQST
                     VIPLRDSKKLPNHKVLLITTGAQGETNSAMGLISRGKHSDVSIKNGDTIVISSSPIPG
                     NEIKYSHMINNLFRQGGQVIYQTISLVHVHGHGSREDLKRMIELVKPRYFIPIHGETR
                     HLVAHADIAEKNGVNRKNIFMIEDGETVEFTGSEAILGSKVHSGTIYINSSGTPKPQ"
     gene            complement(4199..4834)
                     /locus_tag="QF838_06365"
     CDS             complement(4199..4834)
                     /locus_tag="QF838_06365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HJO60339.1"
                     /translation="MSNLEPITITDLEHTVSQCNRCSLSKSRNCTVPGEGNYDAQVMV
                     IGEAPGSNEDKTGRPFAGRAGNLLNELLDSVGIKRPQIYITNMLKCRPPSNRDPQQSE
                     IDSCNPYLDLQISLIKPSVIITLGRFSFSKFLPNIPLSKARGVVSEWNGIKILPIYHP
                     AAALYNPSLKPKLLQDFQKITSLISMDEPVVFEQSQIIPATQLSLFDDHFE"
     gene            4952..5797
                     /locus_tag="QF838_06370"
     CDS             4952..5797
                     /locus_tag="QF838_06370"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HJO60340.1"
                     /translation="MLRKTGILGYPLGHSISPHFQNAAFEFSGIRASYSAWPTEPKNL
                     SDKVDTLRETSFMGANVTIPYKEAVIELLDEIDDWAEKIGSVNTIVNDCGTLVGYNTD
                     AVGFIQAISKYGNYDPAGESVLLIGAGGASRAAAHALVGNNVGTLVIANRTYDRAEKL
                     AAEFSNKDKVSPISLNGPSFLKAVDSASLIINSTSMGMKHTSLEDGNPLEGISVKRGV
                     LVNDMVYNPLRTPMLIHAAERGAKILTGLPMLVLQGAASFNLWTGAEAPVDVMFSAAQ
                     GAMAD"
     gene            complement(5794..>6884)
                     /locus_tag="QF838_06375"
     CDS             complement(5794..>6884)
                     /locus_tag="QF838_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HJO60341.1"
                     /translation="FITSEMKDYESIILNASSKIEEVEKRLFEKICSDISMHLEALLQ
                     TSHAISLMDAFYSLSRIATERNYIKPILNNSDTINIKSGRHPVIEQIVGPGGFVPNDT
                     NLSNTLNQILLITGPNMSGKSTYLRQVGILTLMAQIGSFIPAESATIGIVDRIFTRVG
                     LQDDLTKGQSTFMVEMLETASILNQATGKSLLILDEIGRGTSTYDGLAIAQAVTEFIH
                     EEKTLGCKTLFATHYHEMTELSKRLERITNLHVAVSDINGEIIFLRQLLTGGADKSYG
                     VHVAKLAGLPPKIIGRAWEILAMLENNKSSIASTLNEGAGKPFQTQMFAHRNWLEEEL
                     SNTDIDAMTPLEALIKLQSLKDQTKNFN"
BASE COUNT         1849 a         1377 c         1443 g         2215 t
ORIGIN      
        1 cctttgataa acatttgatt tgtttaggtc actactgcac atcaaacaga atttattctc
       61 gtcctgaact gccaggtcgg attcattcct attcaataat cgcgacatca tacttgaaaa
      121 attactaacc ataatgccct aatattactt tagtgggaag gtaatatcta tttttaggtg
      181 gaagaaatca catctcggga ttttgaacca tcactaggac ccacaacacc catatcttct
      241 aattcatcca tgagacgtgc agccctagga tatccgatcc taagcctccg ttgaagcaag
      301 gaggtggaca ttttggtata tttttgggca agggccattg ctttttcaaa caactcgtcc
      361 tgattgtttg ccccaccggt atcagacatg ccagcaatgt ctgagttttc atcattaacg
      421 atgtttaggt taatttctgg tttatatccc cgcggcgtac ttgaccaatg ttgaactata
      481 tctccaatct catcgtcccc tataaaaaca ttttgtactc tcgcaggtct tgtggcatct
      541 atcggctggt aaagcatgtc acctcgcccc aataatcgtt ccgctcccat cgtgtcaaga
      601 atggtccgag aatctatttg ggaagttaca gcgaagctaa tgcggcttgg gaaattggcc
      661 tttatcagtc cagtgatcac atccacagag ggccgttgtg ttgcgactat caaatgaatt
      721 cctgtggccc tacctaattg tgctaatctg catatagatt gctctatatc gaaggcagcc
      781 gtcatcatca aatcagccaa ctcgtctatg gcgactacga tgtaaggcat ttttgcaccg
      841 cctttactgt taaaggaagc tatattccgt acccctgttt cctccatacg ccgataccta
      901 ttcatcattt cttggatcaa tccctttagg atgcctacaa ccttatcggt ttcgactatg
      961 acaggagtca ttaaatgtgg gataccgttg tacggagtca gttctactct tttcgggtct
     1021 atgagaagca tctgcatttc cgaaggagtt ttttccataa ttaggccgct tattattgcg
     1081 ttgatacaaa cacttttgcc acttcctgtg gctcctgcga tcaacaaatg aggcatcttg
     1141 gatagatcaa aggaaacttc ctctccatca gaaccttttc caagagctac cggtaattga
     1201 cttttcttgg ccaatgaggt gtagggtggg ctttccatca cattttttaa tgcgacaaga
     1261 ctcggggtat gattgggcac ttcgatacct aaaagagatt cccccatcac aggagtttcg
     1321 attctaagac taggggtttt tagagctaat gataaatcct tctcccgacg cagaatgtta
     1381 tccacactaa ccctggtttt ttcttcctgt tgcaccatga caggtttccc atttgtgtct
     1441 atttcaaggt tgccatcctt gtctctagat ttaactctct ttacttttct gacccatccc
     1501 ggttgaatcc cgtacatggt tacagttggg ccaggcctga ctttttctac cgctatttct
     1561 attttgtagt ctgcgaatgt gtttttgatg atgtctgcag tgtcgcgtat ttcttccttg
     1621 ctgatacctc cttctgatat accagaaagc agtttcatat ctggaagctt ccaagaggtt
     1681 gagacactgg ccggatttgg gagaggggtt tttaaatctt ggtcaatctc cccatcaagg
     1741 ttttgctcag ggttggttaa attcgtagtt tgggagtcta tttctaccga ttgggtctct
     1801 ctcgggtccg atttagacca gtatttattg aatttgttgg attcagacgt ggtattccca
     1861 ttatttataa gatctggctc cgattgaatg gattctaaga ccgtttcgtt tacagtggca
     1921 gtagattctt cttcagtttc atttagttga tatacagtta cattggatga agaaatatag
     1981 ctagaaagga gatcagtggt agtgggagag gaatcagcta ggattggctc tttattggct
     2041 atttcaatga gagaattttc tgtgctaggt tttttcgcgg cagacgcgtt tttgataatg
     2101 tggctctcct tcatatgagg agtcagaact tcaggcggga taggtacaaa gggcttggat
     2161 cctatttcct gaggaatatt ctctgtcgac tttttacgga aaatgttcaa atatacgaat
     2221 ttcgaagcca atcctaatga ccaaatggtc agtattatga tctgccaaga tttgactgaa
     2281 ataggcggtt tgattattac taaccctgcg tagaacaata aaaatattct taaccaaccc
     2341 attaatgcgt ttccaccaat tatatgaagt ccaacgtctc caccgatcga gcctcttccg
     2401 atatttgaaa tggtttcaac atcagtaaaa tggataaagc ttatcgcccc ccacaaagat
     2461 ccagttatta ctaaggaccc aacaatctct ttagggtaac tcgagcctac atgctttaat
     2521 ttgtgtaaac cagacactat accaagggca attaaccaga ggcatgggag aataattcca
     2581 agtccagtcc atgcgacgaa agaacttatg taaccagtaa ggaacccggg aattagcgat
     2641 tcaaaaaagg cagttgcaat tatcgagctg gcacatacgg ctgcaattgc aacccaaaat
     2701 aaaataatcg aggggttaaa tgggatgatg ttaggttgag taccgtcaat gttttcgttc
     2761 ataatatgcc tatgctgtgt gcctaaacag gaataggagc aaataaatca tttagtttag
     2821 tctagtaaaa cataggcaac ttaggctagc ggagtttgca ctaaattatt ggggttttgg
     2881 tgtgcctgaa ctgttgatat atatggtccc agaatgaact ttgctcccca atattgcttc
     2941 ggagcctgta aattcgacag tctctccatc ttcaatcata aaaatatttt tacggttcac
     3001 tccatttttt tctgcgatat cggcgtgagc cacaagatgt cttgtttccc cgtggatggg
     3061 tataaaatat cttggtttaa caagctctat catgcgtttt aaatcttctc tactgccatg
     3121 tccgtggacg tgaacaaggg aaatggtttg atatattacc tggcctccct gtcggaacag
     3181 attatttatc atatgggaat atttaatttc attaccgggg atcggagacg atgatataac
     3241 tatggtatcc ccgtttttta ttgatacatc actatgcttc ccgcgggaaa tcaatcccat
     3301 cgcagaattg gtttcacctt gtgcaccggt tgttataagc agaactttgt gatttggtaa
     3361 tttctttgag tccctgagcg gtataacggt gctttgaggg tcagttacat atcctagatc
     3421 taaagcgact ttgagattcc tattcatgct tctacctaca accgatactt tacgaccgat
     3481 tgccgaggaa atagtgatga tttgttggat tctagaaagt aaggaggaaa atgttgcaat
     3541 taatactcta ccttcagatt gcgagattat tttgtttaat tcctcctcca atgtagcttc
     3601 agaatccgta aaacctgcct tttcggcgta agtcgagtcc gaacacaaaa tcagattagt
     3661 atttgaggat aaatttgata gatattcgag atcaacagga ttcccaagtg tcgggttatc
     3721 atcgaactta aaatccccgg tatgtaccaa tgaaccaaca gggcttttta tcgttatacc
     3781 tgcagcgtca ggaatcgaat ggcatacggg aaagaattca aattcgaagc accctaaaga
     3841 atatgactca taaaaattta tagtacgtaa gtcaacttta tttttattac caagtttgaa
     3901 cttgatgaat tctctagtta atctacttga atatacgggc acattatatt catttagaaa
     3961 gtgtggtaac ccaccgatgt gatcgtcatg cccatgagtg attagtacac ccaaaatcat
     4021 atttttcctg tcgtttaaat agcttgtatc tgataccccg aaactggata ctggcttgat
     4081 ttcagaggga aaagctattc cggcatcaat tattaggatc tggccatcat attcgatggc
     4141 catcatgttt ttgcctattt cgccaaggcc tccgagaggt attatcttca aatttttgtt
     4201 attcgaagtg atcatcaaac aaacttagtt gagtggcagg aattatttgt gattgctcaa
     4261 aaacgaccgg ctcatccata ctgattaagg aagtgatttt ttgaaaatct tgtagcaatt
     4321 tgggtttcag acttggatta taaagagcgg ctgccggatg atagattggt aatatcttaa
     4381 taccgttcca ttcgctaaca actcctctgg ccttacttaa tggtatgtta ggaaggaatt
     4441 ttgaaaagga aaatcttcca agcgtgatta tcactgaagg tttgattaag gatatctgca
     4501 ggtctaaata tgggttacat gaatcaatct cgctttgttg cgggtcccta ttgcttggtg
     4561 gtcggcattt gagcatattt gttatgtata tctgaggtct ttttataccg actgaatcaa
     4621 ggagttcatt tagtaagttt ccagccctac cggcgaaagg tctacctgtt ttgtcttcgt
     4681 tagaaccagg ggcttctcct atcaccataa cttgtgcatc ataattacct tcgccgggta
     4741 cagtacagtt tcttgatttt gataagctac atctgttgca ttgagagact gtatgctcta
     4801 agtcagtaat tgtgattggt tctaggttac tcaatttgga gatttcacct tgttttgttg
     4861 aagtgcttct tatattaact tatatttctt tcatctagac ttggtttgcc gtatatcaga
     4921 attgatctta actcttttat ggatttagtt tatgttaaga aaaactggaa tcttaggata
     4981 tccattggga cactcaatat caccgcattt tcaaaatgca gcttttgaat tttcaggcat
     5041 cagggcatcc tacagtgctt ggccgaccga gccaaaaaat ttgtctgaca aggtggacac
     5101 tttgagggag acctctttta tgggcgctaa tgtaacgatt ccctacaaag aagcggttat
     5161 agaactgctg gatgaaatag atgattgggc ggagaagata ggttcagtca acaccatagt
     5221 taatgattgc gggactcttg ttggatacaa cactgacgcg gtgggattta ttcaagccat
     5281 ttcaaaatac ggaaattatg atcctgcggg agaatcagtg ttgttaatag gagcaggagg
     5341 ggcctccagg gctgcagctc atgcgttggt aggaaataat gtagggactt tggtgatagc
     5401 caataggaca tacgataggg ctgagaaatt agcggctgaa ttttctaata aagacaaagt
     5461 tagtccaata tcattgaatg ggcccagttt tttaaaggct gtagattcgg catctctgat
     5521 catcaattca acgtctatgg gtatgaaaca tacctcactt gaagacggta accccctaga
     5581 aggtattagc gtcaaaaggg gtgttttagt aaacgatatg gtttataacc cacttagaac
     5641 ccctatgttg atacatgcag ccgaaagggg agccaaaata ttgacaggtc tacctatgct
     5701 tgttctacaa ggagcagctt cttttaacct ttggacaggt gcagaagcac cagttgatgt
     5761 aatgttttcg gcggcccaag gagcaatggc agattaatta aagtttttgg tttgatcctt
     5821 cagtgactgc aatttaatca atgcctccag tggggtcata gcatctatgt cagtgtttga
     5881 cagttcttct tctagccaat tacggtgagc gaacatttgg gtttggaagg gttttccggc
     5941 accttcattc aatgtagatg caattgaact tttattattt tccagcatcg ctaagatttc
     6001 ccatgccctt cctatgattt taggcgggag tcctgccagt ttggctacat ggacgccgta
     6061 acttttatct gctcccccag tcagaagttg tctcaagaat attatttcac catttatatc
     6121 tgagacggct acgtgcaagt tagttattct ttcaagacgc ttagataact cggtcatttc
     6181 gtggtaatgg gtggcgaata aagttttaca tcccagtgtt ttttcctcat gaataaattc
     6241 tgttactgcc tgagctattg caagtccatc atacgtgcta gtgcctctac caatttcgtc
     6301 aaggatgagc aaggactttc cggtcgcttg attcaatatg gatgctgtct ctaacatttc
     6361 caccatgaaa gttgattggc cctttgtaag gtcatcttgt aaccccaccc ttgtgaatat
     6421 cctgtctaca attcctatcg tggcggattc agctggtatg aaactgccga tttgagccat
     6481 caatgtgaga ataccaactt gacgaaggta ggtagattta ccagacatgt ttgggccggt
     6541 tatcagtaat atttggttaa gtgtattcga taagtttgtg tcgttaggca caaaccctcc
     6601 tgggcccact atttgttcaa ttactggatg tcttcctgat ttgatattga tcgtgtctga
     6661 gttattaagg atcggtttga tgtaattacg ttccgtggca attcttgata gggaatagaa
     6721 tgcatccatg agtgaaatcg catgtgatgt ctgaagtagc gcttccaaat gcatagatat
     6781 atcggaacaa atcttttcaa ataatctttt ctcgacttct tctatttttg aacttgcgtt
     6841 gagtatgatt gattcatagt ctttcatttc tgacgtgatg aaga
//
LOCUS       DASCNS010000177         3053 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001400070, whole genome shotgun sequence.
ACCESSION   DASCNS010000177 DASCNS010000000
VERSION     DASCNS010000177.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001400070"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="QF838_06380"
     CDS             <1..692
                     /locus_tag="QF838_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HJO60342.1"
                     /translation="HEVTHILVHRAGDSVFSGIPSWLNEGLAEFGNVSPSFSYDVALD
                     FAVHADRLMPITLMRNRPGDPEDVIIFYGSASSIVEFMIHKFGPEKMLNLLTEHKSGR
                     NMDDSIEFIYGITKLELENQWRNYIGAPAYEPELLEKKLPTPIPRPTVGLFSLTPQSG
                     SYSIKSTEIDTSENLSSYDEPNDRVSTDIQDPDAAQSTGCNRLDTKSSVTDYAMFLLI
                     IPPLLMWSRRR"
     gene            744..1445
                     /locus_tag="QF838_06385"
     CDS             744..1445
                     /locus_tag="QF838_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60343.1"
                     /translation="MSLSSDWYKAIEQTYVVKYPKQHLATFGVTSIEYFVVTEPLYTA
                     IDSQKKELEGVVRKGKVKAEQPTLITPTYAMNLNGFSDEAYKYLNEVAHLYGANSPGI
                     MYQYNNEPENLEILSGNPTAIAHRISKELREKKNDLSVVISGVDEFWDVALLKFIYEF
                     TASSVSFNSREMRGAGLMEPQSSAGGVPTAAVNQIEEMFKSVKKGGSAETLKNELDKW
                     GIYPYYEDRFLNLFR"
     gene            1595..2647
                     /gene="hflK"
                     /locus_tag="QF838_06390"
     CDS             1595..2647
                     /gene="hflK"
                     /locus_tag="QF838_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HJO60344.1"
                     /translation="MYRQEEPEVNIEQIIARIKGLFGGSSGGSNKGSSKAPILILGII
                     LLGVIGWFATGFFSVQPGEEAALRMFGKYSDTRGTGLQWWWPGPIGAKDIVRVDEIRR
                     LELGVRAGTPVLDESLMITGDPDEQGAPGEAPNIVDVQLLVQYDIKNVKDYLYRVVSP
                     DAFTLKDATETSLRQVVGSRPIDDVLTDNKEIIQIETKAKLQDILDQYQSGIRIREVK
                     LLYVFAPEQVKDAFDDVVRAKEDKARIINLADAYKESVLPQARGTAAKALQDAEGTRQ
                     QDIAVAEGEAQRFLAIQKEYAKSKDVTRKRLYLEAMEDILPGVGKILGNPDEVILVNP
                     DNVSNVMPVPVSGGQE"
     gene            2655..>3053
                     /locus_tag="QF838_06395"
     CDS             2655..>3053
                     /locus_tag="QF838_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013324.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HJO60345.1"
                     /translation="MKLLSILLVALIIVLAIMLPQIFFSVSETEVGIVTRFGQIKSEI
                     TTPGLRIKTPFIDQVTTFEKRLLLFDAPPDSLLTRDKKRLIIDVYARGRIVDPRVFKE
                     RLGDEQRATDRAVDIISSELRREIGVHNQAE"
BASE COUNT          940 a          558 c          718 g          837 t
ORIGIN      
        1 ctcatgaagt gactcacata ttggtccatc gagccgggga tagtgtgttt agcggtatac
       61 cgtcatggtt aaatgagggg ttggcagaat tcggaaatgt gtcccccagt ttttcttatg
      121 atgtggcact tgattttgcg gttcatgcgg atagattgat gcctattaca ttgatgagga
      181 accggcctgg ggatccggaa gatgtgatta ttttttatgg ctcagcaagc tcaatagtag
      241 aatttatgat acataaattc ggacctgaaa aaatgcttaa cctcttaacc gaacataaat
      301 ctgggaggaa tatggatgat tcgatagagt ttatttatgg catcactaaa cttgaacttg
      361 agaaccagtg gagaaactat ataggtgccc ctgcatatga accagaattg ttagagaaga
      421 agttacctac tccgataccc agaccaaccg ttggactttt ttctttgacc cctcagtctg
      481 gctcctattc aataaaatct acagaaatag atacctctga aaacctatct tcatatgatg
      541 aacccaatga cagagtgagt actgatattc aggatccaga tgctgcgcaa tccacaggtt
      601 gcaatcgatt agatacgaag agctctgtaa ccgattatgc gatgtttctt ttgattattc
      661 caccattgct tatgtggtcc cgtcgcagat aaacctgcca ttgatacaat agtacagtta
      721 tttatgttta gggcgtgtaa taaatgagct tatcttccga ttggtacaaa gcgatagaac
      781 agacatatgt tgtcaaatac ccaaagcagc atttggctac tttcggcgtt accagcatag
      841 aatattttgt agttactgaa cctttgtaca cagcgattga ttcgcaaaaa aaagaactgg
      901 aaggggtggt tcggaaaggc aaagtgaagg ctgaacaacc aacgttaatt actccaacct
      961 atgctatgaa tttgaatggg ttcagtgatg aggcgtataa gtacctcaac gaggtagcgc
     1021 atctatacgg tgccaacagc cctggtatta tgtatcagta caataatgaa cctgaaaatt
     1081 tggaaattct tagcggcaac ccaaccgcga tcgcccaccg tatctctaaa gagctcagag
     1141 aaaaaaagaa tgatctctct gttgttatat ctggagtgga cgagttctgg gatgtagctt
     1201 tgcttaaatt catatatgaa tttacagcct catctgtttc tttcaacagc cgagaaatga
     1261 gaggtgctgg actcatggaa ccgcaaagtt ctgctggagg agttccgacg gcagccgtga
     1321 accagattga ggaaatgttc aagtcagtga aaaaaggtgg aagtgcagag actctaaaga
     1381 atgagttgga taaatgggga atttatcctt attatgaaga tagattttta aatctatttc
     1441 gataaagctc cttaaaaaat attaattttc tattcttcca gatgcaatga cttttacaga
     1501 ctccacttaa atggcctctc ctttaagata tatttagtct tttctttatc cttcctatgc
     1561 tagaattaag cttacaaaat gtacgcgggg caatatgtat agacaagaag aaccagaagt
     1621 taacatagag caaattattg caaggattaa ggggttgttt ggtggctctt caggtggtag
     1681 taataaaggg tcaagcaagg ctccgatact tatccttgga ataatccttt taggtgtcat
     1741 cgggtggttt gcaacaggat tcttttctgt tcaacctggg gaggaggcgg ctctgcgaat
     1801 gtttggtaaa tattctgaca ccagagggac aggattacag tggtggtggc cgggaccgat
     1861 tggggcgaaa gatatagtcc gggttgatga aatacgtagg ttagaacttg gagtacgagc
     1921 agggactccc gtgcttgatg aatcattgat gatcactgga gacccagatg agcagggagc
     1981 gccgggagaa gcacctaata tagttgatgt acagctattg gttcaatatg acattaagaa
     2041 tgttaaagac tacctctaca gagtggtctc accagatgct ttcaccttga aagatgccac
     2101 tgagacatcg ttgagacaag ttgtcgggag taggcctatt gacgacgtct taaccgataa
     2161 caaggaaatt atacaaattg agaccaaagc aaagcttcag gacatattag atcaatacca
     2221 aagtggtata aggatcagag aagtaaaatt gctgtatgta ttcgctccag aacaggtgaa
     2281 agatgcattt gatgacgttg ttagagcaaa ggaagacaag gctcggataa tcaacttggc
     2341 tgatgcatac aaagaaagtg ttttgcctca ggcaagaggt accgcagcga aagcattaca
     2401 agatgcagag gggacaagac agcaagatat tgctgttgcg gaaggagaag cacagagatt
     2461 cttggcgatt caaaaggaat acgcgaaatc aaaagatgtg acacgaaaac gcctctatct
     2521 agaagctatg gaagatatac tcccaggtgt cgggaaaata ttggggaatc cagatgaagt
     2581 gatattagtc aatccagaca atgttagtaa tgtgatgcct gttccggtat ctggcgggca
     2641 ggagtagcac cataatgaaa ttactctcta ttctactagt tgcactgatt atagtattgg
     2701 ccattatgct gcctcaaatc tttttctctg tgagtgaaac agaagttggc attgttacac
     2761 gattcggcca gattaaaagc gaaataacga cccctggttt gaggattaag acaccattca
     2821 ttgatcaggt gacaacattt gaaaaaaggc ttttgctttt tgatgctcca ccagactcct
     2881 tactgaccag agataaaaaa cgtctcatta tcgacgttta tgcccgagga cgtattgttg
     2941 atccaagagt tttcaaggaa agacttggtg atgaacagcg tgctaccgat cgtgcggtgg
     3001 acattatttc ttcggaactc agacgagaaa taggtgttca taaccaagcg gag
//
LOCUS       DASCNS010000178         7128 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001408297, whole genome shotgun sequence.
ACCESSION   DASCNS010000178 DASCNS010000000
VERSION     DASCNS010000178.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001408297"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            69..383
                     /locus_tag="QF838_06400"
     CDS             69..383
                     /locus_tag="QF838_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HJO60346.1"
                     /translation="MPDSETVTKDDVLEALRDVYDPEIPVNIVDLGLVYGVEVAEGDV
                     DVSMTLTFAGCGMGPYIAQQAEWRLAEVEGIEDINVDLVFDPPWTPDMITEEGKRLLG
                     LD"
     gene            442..1248
                     /locus_tag="QF838_06405"
     CDS             442..1248
                     /locus_tag="QF838_06405"
                     /inference="COORDINATES: protein motif:HMM:NF022077.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HJO60347.1"
                     /translation="MTQQNPAQARARIEAMKRQFDQKRQIEESLSGIKNKIGVYSGKG
                     GVGKTTVAVNLAATLANDGATVGILDVDIDCPNVVRAMKIAEHPTVGGEQKMIPPERF
                     GVKVMSMSFFQENEDEAIIWRGPMIHNAINQMLQTTEWGELDYLVVDMPPGTSDAPLT
                     VMQVLTMDGFVVVTTPQELAKIDAKRSINMIRKLQINVLGVVENMSGGIFGQGAGEEI
                     SKELDLEYLGDINVRADYQDTSRPASLLNNEILDEYRNITGKTLKALNRR"
     gene            complement(1253..2851)
                     /locus_tag="QF838_06410"
     CDS             complement(1253..2851)
                     /locus_tag="QF838_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012562.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60348.1"
                     /translation="MYSKQYFSNWDEDILLDSIAKFWESAERSTPIDNHAFMSNWLGW
                     SLGCDWTLDEKRQYVELANEICGEDFQYILLVGVGGSSAGARAIASLGDNSPKFVFVD
                     SVLPSVVEKIAAEVGSKKTLLVISSKSGTTLETITLANYFLDKLGDSREPVTYKKNAI
                     AITDPGTFLEELAIKQNFRSIIRGKPDIGGRFAELSCYGLFPALLNRTDISAILDSAN
                     EMAYLCRTVTLEDNPAIPLIEFLLQNLRDGRDKLYVYLPESLSNFAMWLEQLLSESLG
                     KDGVGIIPFIGESDLVSPSLDLCSVMYQDSSMEDTATRNLRQCFEDHSIPIFNIKVNK
                     PESFGGEFFRWKMSVVLMAYSMKINPFDQPQVQSSKDKTGEILDYFTNHGKLPPTCST
                     LTLPEWTAQIKNDTYIAVLPFLQIGDEDQELLFNLASRMRKLTKRHVTLAMGPSYLHS
                     TGQMHKGGPESGMYLQLSSDNVGEIPIAGEKYGFSTLLAAQADGDMESLVSLNRPVCR
                     VNLGSDITQGLNKLIAQFQTLYEQ"
     gene            complement(2867..3976)
                     /gene="tal"
                     /locus_tag="QF838_06415"
     CDS             complement(2867..3976)
                     /gene="tal"
                     /locus_tag="QF838_06415"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HJO60349.1"
                     /translation="MSEASRLLELKKLGQSVWYDNISRDLLLSGGLKKILDSGVVGLT
                     SNPSIFEKAIASSNIYDSDIQNMKDKKYSDLEVFEKLAIEDIQNTADLLSDTYRSTEG
                     QNGYVSLEVNPHLANDTKGTVDEAKRLYSEVDRQNLMIKVPATKAGIPAIHELISIGI
                     SVNVTLIFSVQMYDLVREAYISGLEQFHKNAADLSKVTSVASFFVSRVDTATDKKILE
                     QGFAPEGFSGRAGIANAKIAYVEFEKTFSSDRYLQLRDAGAKIQRPLWASTSMKNPEI
                     RDVLYVESLIGPNTVNTMPDVTLNAFIDHGIAETTINENINDSYAHMEKLASAGIDLK
                     EITDTLLEEGVKAFADSFDELLQNISHKRSMLSVS"
     gene            complement(4177..>7128)
                     /locus_tag="QF838_06420"
     CDS             complement(4177..>7128)
                     /locus_tag="QF838_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HJO60350.1"
                     /translation="DGLYKGTWKSSTLACRFFAESSTLLESLSFQQLENFANFLDVLS
                     HRSYDLSSECLTLGEQIFPLVGDDKDAFLSLATTLVDTGWREVKSFFEAGAKALPKIH
                     PEERMRFLKLAESLVNNGGTNIPGTMLEISQSLSLLDEDNHHIVLGFAEALLDEEPLA
                     MPEFIKSAPIVLEKLTILQLGRWYQEGVNTLSQNKDGGLAFFKIESSHSEAVIETLSS
                     GIEFDRIKPVMEMYCRALAGAEIKLSPTDALVEKNIGWVSNESPTTEGSTVFVPTLVD
                     RYGTKEENYAWFKVVSTHQVAHLEFGSFIFEFERQSGLFENLRFDLETRRIETLKEKQ
                     NKESAVGDDVTDSDFSGDELNDAHTGLERAWLTDMQRFFDLFEDRTLSLDIFTVVEDG
                     RLDARIKNEYPGIKSSYTQVQSDSHENRPDIKELPAREAMVEFMVRLSLQQYEGVAAP
                     SKYNKEASQIAQIARRVLVPEATVEDTAEATLRIYAIISAIPNEEIPPDDWNDMDFED
                     EDEEEYVDPEDMENLLQQIGMGMEMELRPEGEEEYESTQEVEYRGDFKPELAQLMTEL
                     RMQQQDEMSQGQGEPLTQEQLQELLENSAELDLQATEGEIQESSGMFADNIMKEAGAN
                     MPQSPDDSQGPFVHVDEDGGELEPAEPETFVYDEWDFRAEDYKPRWCVVRQKMMQEGD
                     PAYFGATLNEYSTLVTRIRRQFEMMVPEMFRKERKLIDGEDIDIDDVIENMVDIKTGS
                     SPDEKFYWRRNKVQRDVAVAFLLDTSASTAEAIDEGKNKQDDWDAPDDPVEYMVWLRT
                     RRGEQMRRSYKRIIDMEKESMVLLINALEAIGDTYGIYGFSGYGRENVEFYTVKDLHE
                     SFSDKVKRRIDRIAPLHATRMGPAIRHAAYKLDNIDARTKILFLISDGRPQDRGYSRE
                     GVEKEYAVHDTKKALDEAKRQGINSFCLTVDKNGHDYLKTMTSDMGYEILDDINDLPE
                     RLLVLYRRLTM"
BASE COUNT         2010 a         1586 c         1377 g         2155 t
ORIGIN      
        1 attgcggtaa gtgacggaat tgcaatgggg catgagggaa tgaaatcatc tttagtaagt
       61 cgagaagtat gcctgattca gaaacagtca caaaagatga tgtattggaa gcccttcggg
      121 atgtctatga tccggagata ccagtgaata ttgtggactt gggattggtg tatggagttg
      181 aagtggcgga gggtgatgtc gacgtaagca tgacattaac ctttgccggt tgtggaatgg
      241 gaccttatat agcccagcag gcagagtgga gactagctga ggtggaaggt atagaagata
      301 taaatgttga ccttgtattt gacccgcctt ggacgcctga catgataacc gaagaaggaa
      361 aacggttact cgggctcgat tgaagacctc attcttttca aggtaacggg ttcggtaaca
      421 agcagactag cggagaattt catgacgcaa caaaacccag ctcaggcacg cgctcgcatc
      481 gaagcaatga aacgtcagtt cgatcagaaa cgtcaaattg aggaatcatt atcaggaatc
      541 aaaaataaaa tcggagtata cagtggaaaa ggtggagtag gtaaaactac cgttgcagtt
      601 aatttagctg cgactctagc taatgacggt gccaccgttg gtattttaga tgtcgatatc
      661 gattgcccta atgtagtgcg agcaatgaag attgccgagc atcctacagt cggtggggaa
      721 caaaaaatga tcccaccaga aagatttggg gtcaaagtta tgtctatgtc atttttccag
      781 gaaaatgagg atgaggcgat aatttggcga ggaccgatga tccataacgc tatcaaccaa
      841 atgctccaaa ccaccgaatg gggggaactt gactatttag ttgtagacat gccccctggc
      901 acttctgatg ctccattaac agtgatgcaa gttctaacaa tggatggctt cgtagttgtt
      961 accacaccac aggaactggc caaaattgat gctaagcgct ccataaatat gattcgcaag
     1021 ctacagatca atgtgttagg agtagttgaa aacatgtccg gtggaatatt cggccaaggt
     1081 gcaggtgaag aaatatcaaa agaattagat ttggaatatc ttggcgatat aaatgtcaga
     1141 gcagactatc aggacacgtc acgaccagct tccttactaa ataacgaaat actagatgaa
     1201 taccgaaata ttacggggaa aaccctcaaa gccttaaacc gcagatagcc gttcattgtt
     1261 cgtataaagt ttgaaattga gctataagct tatttaatcc ctgggtgata tcgctaccga
     1321 gattcactcg acataccgga cggttcaaag acactagtga ttccatgtca ccatctgcct
     1381 gagctgccag taatgttgag aatccatact tctcacccgc aatcggaatt tcacccacat
     1441 tatcagaaga aagttgtaga tacatcccgg attccggtcc ccctttatgc atttggcctg
     1501 ttgaatgcaa ataagatggc cccatggcta gtgtcacgtg ccttttagtg agctttcgca
     1561 ttctagaggc caaattgaaa agtagctctt gatcttcatc tccgatttgt aggaagggta
     1621 atacagcgat gtaggtatca ttttttattt gtgctgtcca ttcggggaga gtcaaagttg
     1681 aacaagtagg gggcaatttc ccatgattag tgaaataatc gagaatctca ccggttttgt
     1741 ctttagaact ctgtacctga ggttgatcaa atgggtttat cttcattgaa tatgccatta
     1801 aaaccactga cattttccat ctaaaaaatt cccctccaaa cgactctggt ttatttacct
     1861 ttatattaaa tatgggaatt gaatggtctt caaagcactg tctgagattt cgagttgctg
     1921 tgtcctccat tgatgaatcc tgatacatga ctgaacagag atctagtgaa ggagacacca
     1981 aatcagactc accgatgaac ggaataatcc caacaccgtc ttttcctaaa ctttcagaaa
     2041 gcaattgttc aagccacatt gcgaaattac ttagtgattc aggcaaatac acataaagtt
     2101 tgtctctgcc atctcttaag ttttgaagca agaattcaat caaaggtatt gccgggttgt
     2161 cttcaagggt aactgtacgg cataaatatg ccatttcgtt ggcggaatca agaattgcgg
     2221 atatatctgt cctattaagt aaagcgggaa acaaaccgta gcaagataat tcggcaaacc
     2281 tgccaccgat gtcaggtttg ccgcggataa tacttctgaa attctgtttg atagccaatt
     2341 cttcaaggaa agttccaggg tctgtaatgg caatggcatt ctttttatat gtcaccggtt
     2401 ctcgtgaatc gcccagttta tccaaaaagt aattagcaag tgtgatggtt tctaatgttg
     2461 ttcctgattt ggaagaaatc accaaaaggg tttttttgct tccgacttca gctgctattt
     2521 tttcaacaac agagggaagc acagaatcaa caaaaacaaa tttgggagaa ttatcaccta
     2581 aagaagctat tgctctggca cctgcactgc tacctcctac cccgaccaac aatatgtatt
     2641 ggaaatcctc tccacaaatt tcatttgcaa gttccacata ttgtcttttc tcatctaaag
     2701 tccaatcgca gccgagtgac caccctaacc aattcgacat aaaggcgtga ttatctatgg
     2761 gtgttgatct ttcggcagat tcccaaaatt tagcgatact atctaatagt atgtcttcat
     2821 cccaattgga aaaatattgt ttagaataca taatccaaaa taatcatcaa ctcactgaca
     2881 gcatggatct cttgtgcgat atattttgca gaagttcgtc aaaggaatct gcaaaggcct
     2941 taacaccttc ttcaagtaag gtatctgtta tttctttaag gtctataccc gctgaggcca
     3001 gtttttccat atgcgcatag gaatcattta tgttttcatt gatagtagtt tcagctatgc
     3061 cgtggtctat aaatgcattt aacgtgacat ccggcatagt gtttacggta tttgggccta
     3121 ttaaactttc aacatataaa acatcccgaa tctcggggtt tttcatactg gtactagccc
     3181 ataaaggtct ctgaattttg gctcctgcat ctcgaagttg gaggtaacgg tcagaggaaa
     3241 aagtcttctc aaattctaca tatgctatct tggcatttgc gattccggca cgacccgaaa
     3301 atccttcagg tgcgaatcct tgttccagta ttttcttgtc ggtggcagta tctaccctgc
     3361 taacaaaaaa agaagccact gaagtaactt tagagaggtc ggccgcattt ttatgaaact
     3421 gctcaaggcc gctaatatag gcctctctga ctagatcgta catttgcaca gaaaatatca
     3481 aggtcacatt gacactaatg ccgatagata tcaattcatg tatggcagga atcccggctt
     3541 tagtggcagg aacctttatc atcaaatttt gccgatcaac ttcagaatat agccttttag
     3601 cctcgtctac tgtgcccttt gtatcatttg ccagatgtgg gtttacttca agggaaacgt
     3661 acccattttg cccttcggtt gatctatacg tatcagacag aagatcggct gtgttttgta
     3721 tgtcctcaat tgccagtttt tcaaatactt ctaagtcaga atatttttta tccttcatat
     3781 tttgtatatc agaatcatat atatttgaag aagcaattgc tttttcaaat atactagggt
     3841 tggatgttag accgacaact cccgaatcta atatcttttt tagaccaccg gatagaagaa
     3901 ggtctctact tatattgtcg taccaaactg actgcccaag ttttttcagt tctagtaacc
     3961 ttgaagcctc cgacatagat ttcctctctc tataaacgca ctcaattaat tttttgatgt
     4021 aaggttgaat tgaaccctat caaacacagg aacatgatat caccatggtc ttttagatct
     4081 aaaaagaatt ctaacccgtt taaataattt aaatgctggt caagattcaa aagattatga
     4141 catagcaatt aatttgagta actagggcaa ttgtttctac atagtcaatc gtcggtacag
     4201 aaccagcagg cgttctggaa ggtcattaat atcgtccaat atttcatatc ccatatcgga
     4261 agtcattgtc ttcaagtaat cgtgtccatt cttatcaaca gttagacaaa aagagttaat
     4321 cccctgtctt tttgcttcat ccaatgcctt cttggtgtcg tgtacagcat attctttttc
     4381 taccccttcc ctactataac cgcgatcctg cggtcgaccg tcactaatca agaacagtat
     4441 ttttgtcctt gcgtcaatat tatccagttt gtatgctgcg tgccttatcg ccgggcccat
     4501 tcttgttgca tgcaaaggag cgattcggtc aatcctacgt ttgaccttat ctgagaaact
     4561 ctcgtgaaga tcttttacgg tataaaactc tacattctcc cttccatacc cagaaaagcc
     4621 atatatccca taggtgtctc ctattgcctc caaagcattt atgagaagaa ccatagattc
     4681 tttttccatg tcaatgatcc ttttgtagga tctcctcatc tgttccccac gcctagttct
     4741 caaccaaacc atgtattcca ctggatcgtc gggagcatcc cagtcatctt gtttgttttt
     4801 gccttcatct atggcctctg cagtagaagc actggtatct aaaaggaaag cgaccgccac
     4861 atcacgttga actttatttc gtcgccagta aaatttttca tctgggctgg agccagtttt
     4921 tatgtccacc atattctcga tgacatcatc aatatctata tcttccccgt ctatcagttt
     4981 tctttctttc ctaaacattt cggggaccat catctcaaat tgtctgcgga tcctagtgac
     5041 taaggtgcta tattcgttca gggttgcacc aaagtatgcc ggatcaccct cttgcatcat
     5101 tttctgcctt actacacacc atctaggttt gtaatcttct gccctaaaat cccattcatc
     5161 atagacaaac gtctccggtt cggccggttc caattctcct ccatcttcat ctacgtgaac
     5221 aaatggccct tgcgaatcgt caggtgactg gggcatatta gccccagctt ccttcataat
     5281 attatctgcg aacatcccac ttgattcttg aatttcgcct tcagtcgcct gtaaatcgag
     5341 ttcagcacta ttttccaaaa gctcttgaag ttgttcttgt gtcaagggtt ctccttgacc
     5401 ttggctcatc tcgtcttgtt gctgcattct taactcggtc atcaactgag ccaattcagg
     5461 tttaaagtcc ccacggtatt ccacttcttg agttgattca tattcttctt cgccttccgg
     5521 gcgaagttcc atttccatac ccatacctat ctgttgtaag aggttctcca tatcctcagg
     5581 gtcgacatac tcttcctcat cctcatcctc aaaatccata tcgttccagt catcaggggg
     5641 tatttcttca tttggtatgg ctgaaatgat tgcgtatatc cttaaggttg cttccgcggt
     5701 gtcctcaaca gttgcctcag gcaccaaaac cctacgagca atttgtgcta tttgagaagc
     5761 ttctttattg tattttgacg gggccgcgac tccttcgtat tgttggagac tcagtctcac
     5821 cataaattca accatggcct ccctagctgg aagttctttt atatcaggcc gattttcatg
     5881 tgaatcactt tgaacctggg tatatgaaga cttgatcccg gggtattcat ttttaattct
     5941 tgcatcaaga cgtccatcct ccactaccgt aaaaatatca agcgataatg tacgatcttc
     6001 aaataaatca aaaaatcttt gcatatctgt tagccaagct ctctccaagc ctgtatgcgc
     6061 atcattcaat tcatccccag aaaaatccga gtcagttaca tcgtcaccga ctgcactttc
     6121 tttattttgt ttttccttta gcgtctctat ccttcgtgtt tcaagatcaa agcgaagatt
     6181 ttcaaaaagg cctgactgtc tttcaaactc aaatatgaag ctaccaaatt ctaaatgggc
     6241 tacttgatgc gtagaaacta ctttgaacca agcataattt tcttccttgg tcccatacct
     6301 atcaaccaaa gtgggtacaa atacggtaga tccttctgtg gtgggcgact cgttggaaac
     6361 ccatccaatg tttttttcaa ccagtgcatc ggttggggat agcttaattt cagccccagc
     6421 cagggccctg caatacatct ccataacagg cttgattctg tcaaactcta tacctgaaga
     6481 tagggtctca attacagctt cagaatggga agattctatt ttgaaaaagg ctaatccccc
     6541 atctttattt tgcgatagcg tattcactcc ttcctgatac caacgaccca actggagtat
     6601 agtaagtttc tccagcacta tgggcgctga ttttataaat tcaggcattg ctaaaggttc
     6661 ttcatcaaga agggcttcag caaatcccag aactatatgg tgattatcct cgtctaataa
     6721 ggaaagtgat tgagatatct ctaacattgt tcccggtatg ttagtccccc cattatttac
     6781 taagctttca gccagtttta aaaatctcat cctctcttct ggatgaatct taggtaaggc
     6841 ttttgcgcca gcctcaaaaa aacttttaac ctcccgccag ccggtatcta ccaacgtggt
     6901 agctaatgac aaaaaggcat ctttatcatc ccctaccaaa ggaaaaatct gttcgcccaa
     6961 agtcaaacat tctgaggaca aatcatagga cctatgggaa aggacatcca aaaagttcgc
     7021 aaaattttct aattgttgaa aagaaaggct ttctagtaag gtcgaactct ctgcaaaaaa
     7081 tctgcacgcc aaagtgctgg atttccaagt ccctttataa agcccatc
//
LOCUS       DASCNS010000179         5484 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001418380, whole genome shotgun sequence.
ACCESSION   DASCNS010000179 DASCNS010000000
VERSION     DASCNS010000179.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001418380"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            92..388
                     /locus_tag="QF838_06425"
     CDS             92..388
                     /locus_tag="QF838_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60351.1"
                     /translation="MEEVTLDIRGQGTLRATEIISDLRIVAETFYGPMKMVGFWDYQK
                     DMHLCPHMERRQDCPHTLKEDDPNFVSYTSTLERERHCNLAVSFPHAEITLYLS"
     gene            411..1985
                     /locus_tag="QF838_06430"
     CDS             411..1985
                     /locus_tag="QF838_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJO60352.1"
                     /translation="MPANITRPSLKPYGVYPVHDILTKASLKFPDKTAIIDGNSSYTF
                     SELEEYSSQFSGALKRLGVSKGDRVGILAPNCAEFVIAFHGISRSGAIVSTINSGYRE
                     REIAHQVQDSGCQILVVHESLNEILDEAKKLIKNDVRSIAITSNKATLGSFWELLGQS
                     EAYTATNINPENDLAALPYSSGTTGLSKGVMLSHFNVVSNVEQILGLTGGASMAEDDV
                     ILVHLPLFHIYGMNVLMNPCIAAGSTQVMMGRFDMEEFLDLIETHRVTKLFTVPPVGL
                     GLSQYPGVASRDLSCIKFAMFGAAPLSSELQLKISEVLNCPVIQGYGMTESSPVTNID
                     FTEPDLLKAGSIGPAVSDTEEKIVDVEDPTKELTPGEIGELVIRGPQVMKGYFNNSEA
                     TQETITSDGWLHTGDIGKMDEDGYVWILDRKKELIKYKGFQVPPAELEGLLIEHPEIS
                     DAAVIGKPDVESGEIPKAFVVKTKGSNISEDAVMSFVSSKVATFKYVREVEFIDAIPK
                     NASGKILRRLLKDQQE"
     gene            complement(1994..2503)
                     /locus_tag="QF838_06435"
     CDS             complement(1994..2503)
                     /locus_tag="QF838_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HJO60353.1"
                     /translation="MQKLNRAEVEIILASGSSLAGSDLSETNLSGIDFSSVDMSSCDM
                     RYTDLTGAVLNSTNLSHSNMGAADLSLASLKRANLKNVDLRWAILKNSDFTDSDMQSI
                     GLLEANVSGSILARVNLSDGDLRRTNLSGASLVGANLSRSNLAMADLTGAAIEGVSFN
                     TCNTYGTKF"
     gene            2668..>5484
                     /locus_tag="QF838_06440"
     CDS             2668..>5484
                     /locus_tag="QF838_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012298.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HJO60354.1"
                     /translation="MQIRRIGRYQLLEIVGKGGQGTVYRGHDPSTGQIVAVKILAEAH
                     SDGDFLERFQREASILASLEHPHVIKVFDHGEEEGRHYIVTEYVPENLARIIEQGDPL
                     PTRRALTVAAQIASALAVTHENGVTHRDVKPANVLISDSGDVKLTDFGIATADVMNTV
                     STPDSTVGTPLYMSPEQIQSRDIDGRSDIYSLGCLLYQILTGNPPFQGESAYDIFNAH
                     MNGEYEPLSSVIESCPEELDNLLSKALATSVNDRFQSADELMDAIDEVADQLTDTGDQ
                     TLATRVMPRTIRSLSKRNPIWKRRNTWVTGVAAVAVLAIVGFAASTMLGGGSAVGLAT
                     GVTDTTESSLVPQAPIIIPEEPELAADLPIAENVLFSYGPNPDYVYAQISLPAVADVG
                     HLSNISASTRDLDGNEAKLNVASVKTDGLRAPLNVIFLRAMEITVDAGEVVTDTLPGI
                     IEFQVKRQWIEESGIQKNDIGLYRFKDEWQILPTIHEGSFIGSEDLFYERFSATTPGF
                     SRFAIGLTLQTQDAQSIAPDSSKETASPQQPPADSPIQPSKPQSANEFPAATPLTPTP
                     LALVGLQTTEVPTPEPMPTQSVPTPILAAVTPQTSVEALPTQEPLPTATPIPPTQVPT
                     PTRVPPTSTPTPSPTPVLAEKVKDGSLIQLSTTQQAHIEYPGDVDEWIYVPSSSMIGK
                     SVNISGITDNPDSDGYLEFLSPSGDVVGEDDDSGLNMNPVISNALLSESGVYTIRFML
                     LDGTSGGYTLQIEPADSNLLTPTVTLTPTPTPTPTPTPTPTPTPTPTPTPTPTITPIP
                     PISHTVYFKDSNLEEAVRNSLRNTIGTAPDEITYGDMAELTNLDAYAASTGAITSLVG
                     LEFATNLESLYLDNNSISDLGPISSLTNLRWVEFWGNNISDLGPLSGLTNLESLGLGE
                     NSISDIGPISSLTNLEYLYMDQNSI"
BASE COUNT         1670 a         1242 c         1150 g         1422 t
ORIGIN      
        1 ggttccggaa atgtaaatac tgtggataat tctggcaaca aaatatcaat gacaacaggc
       61 ggatgggacc attttacgga gaattaaaag catggaagaa gtcactcttg atataagggg
      121 ccagggaact ttaagggcca ctgaaattat ttcggacctt cgtattgttg cagaaacatt
      181 ctatggacct atgaaaatgg tcgggttttg ggattaccaa aaggatatgc atttatgccc
      241 tcacatggaa cgtaggcaag attgtcctca taccctgaaa gaagatgatc caaattttgt
      301 cagttacacg agcacgctgg aaagagaacg tcattgcaat cttgctgtat cattcccgca
      361 tgcagaaata acactttatc tttcatgaat agaaacctaa gaggtgtcat atgccagcaa
      421 atatcacaag accttcatta aaaccatatg gtgtttatcc ggtccacgat attctgacaa
      481 aagcgtcgct aaagttccca gataaaaccg ccataattga tggtaatagc agctacactt
      541 tctctgaatt ggaagaatat agctcacaat tttcaggtgc tttgaaaaga ttaggagtct
      601 cgaaaggtga tcgggttgga attcttgccc ccaattgtgc tgaatttgtc atagcattcc
      661 acgggataag ccgctcagga gcaatagtat caacgataaa ttcgggatat cgggaaaggg
      721 aaattgcgca ccaggtgcaa gacagtggat gccagatttt agtggttcat gaatcgctaa
      781 atgaaatcct agatgaagcc aaaaaattga tcaaaaatga tgtcagatca attgctataa
      841 catccaataa agctaccctc ggatcatttt gggaattatt gggccaatct gaagcatata
      901 cagcaacaaa tataaaccct gaaaatgatt tggcagccct tccgtattca tcaggaacga
      961 ctggccttag caaaggggta atgctgtctc atttcaatgt ggtaagcaat gtggaacaga
     1021 tcctaggcct tacaggcggg gcgtctatgg cggaggacga tgtgattctc gtacaccttc
     1081 cgctctttca tatatacggt atgaatgttt taatgaatcc ttgtatagct gctggttcaa
     1141 cccaagtgat gatggggcga tttgatatgg aagagttttt agacttaatc gaaactcatc
     1201 gggtgacgaa gcttttcacc gtaccgccag tgggattagg gttgagtcag tatccaggag
     1261 tggcatcgag ggacttatca tgcatcaaat ttgccatgtt cggagcagca cccctttctt
     1321 ccgaattaca gttaaaaata tctgaagttt tgaattgccc tgttattcag ggatatggaa
     1381 tgactgagtc atctcctgta acgaatatag atttcacaga accggatctt ctaaaagccg
     1441 gctccattgg accggccgta tcagatactg aagaaaaaat agtcgatgtt gaagacccta
     1501 ctaaggaact gacccccggg gagataggag agttggtaat caggggcccc caagtcatga
     1561 aggggtattt caataattct gaagctacac aggaaactat tacgtcggat ggatggctgc
     1621 acacgggtga catagggaaa atggatgagg acgggtatgt ttggattctt gataggaaga
     1681 aggaattaat caaatataaa gggttccaag taccaccggc cgagctagaa ggattgctaa
     1741 tagaacatcc agaaatatct gacgcagccg tgattggaaa accagatgtg gagtccggag
     1801 agattcctaa agccttcgta gtcaagacaa aaggaagtaa tatctccgaa gatgctgtta
     1861 tgtcattcgt ctcatcaaag gtagcgacct ttaagtacgt tagagaggta gaatttatcg
     1921 atgcaattcc taaaaatgca tccggcaaga ttttgaggag actcttaaag gatcagcagg
     1981 agtagaacga tatctaaaat ttggtgccat atgtgttgca tgtgttgaat gacactccct
     2041 ctatagcagc acctgtcaga tcagccatgg ccaaattaga tcgtgaaaga ttagctccca
     2101 caagagatgc tccagaaaga ttggttctcc ttaaatcccc atcgcttaaa ttgacccgtg
     2161 caagtattga tcctgaaaca tttgcctcta gcaatccaat agattgcatg tcggaatcag
     2221 tgaaatcgga atttttcaga attgcccaac gcaaatccac gttctttaag ttagctctct
     2281 tcagtgaagc caaggacaag tctgctgctc ccatatttga gtgactcaaa ttagttgaat
     2341 ttaaaaccgc gccagttaag tctgtatatc tcatatcaca tgaactcata tctacagagg
     2401 aaaaatctat acctgacaaa ttggtttcag atagatctga cccagctaag gaacttcctg
     2461 acgcaagaat tatttccacc tcagcccgat tcaatttctg catagacaaa caccaaccta
     2521 tctctaaaac gtctagttaa caataacaaa caaggtgtat tttattcgaa ctgagtccat
     2581 ataagtgtaa acacccgtga ctaaatcact catatgtaat ctaatattaa gtaagatttt
     2641 atttaatgcc agtaaaacta tactcatatg caaattagaa gaatcggtag ataccaactt
     2701 cttgagatcg ttgggaaagg tggtcaagga accgtttatc gtggacacga cccttccacc
     2761 ggtcagatag ttgcggtcaa aattctcgcc gaagcacaca gtgatggtga cttccttgag
     2821 aggttccagc gagaagcctc aattcttgca tcccttgaac atccacatgt tataaaggtg
     2881 ttcgatcatg gcgaagaaga gggtcgacat tatatagtca ccgaatatgt ccctgagaac
     2941 ttagctcgaa taattgagca aggtgacccg cttccgacaa gaagagcctt aacagtagcg
     3001 gctcaaatcg cctcggctct tgcagtaaca catgaaaatg gtgtgaccca tagggatgtt
     3061 aaaccagcaa acgttctaat ctccgattcc ggcgacgtga aattaactga ttttgggatc
     3121 gccacagctg atgtgatgaa cacagtgagc actcctgatt ctacggttgg aaccccactg
     3181 tatatgtccc ccgagcaaat tcaaagccgt gacatagatg gcagatctga tatttattcg
     3241 ctgggctgtc tgctttacca aattcttacc ggaaaccctc ctttccaagg agaaagtgcc
     3301 tacgacatat tcaatgccca tatgaacggt gagtacgaac cactctctag cgttatagaa
     3361 agctgtccag aagaactaga taatcttctc agcaaagctt tagcgacaag tgtaaatgac
     3421 agatttcaat cggctgatga attaatggac gctattgatg aggtggcaga tcagcttaca
     3481 gatactggtg atcaaacatt agcaaccagg gttatgccta ggacaataag atcgttgtct
     3541 aaacgtaatc ctatttggaa aagaagaaac acatgggtga cgggagttgc ggctgtagcc
     3601 gttttggcta tcgttggatt cgctgccagc acaatgttgg gagggggatc cgccgtcggt
     3661 ctggcaacag gtgtaactga cacaacagaa tcctctttgg tgcctcaagc cccaatcata
     3721 atacctgaag aacccgaatt ggcggctgac ctaccaatcg cagaaaatgt ccttttttct
     3781 tatggcccca atcctgacta cgtatacgca caaatttcgc ttcctgctgt tgctgatgta
     3841 ggacacctat ccaatatctc tgcctctaca agagaccttg acggtaatga ggcaaagttg
     3901 aacgtagcaa gtgtaaaaac agatggatta agggcgcctc taaacgttat tttcctgaga
     3961 gcaatggaaa taaccgttga tgcgggtgaa gttgttacag acactttacc gggcataatt
     4021 gagttccaag tcaaaagaca atggatagaa gaatccggaa tccaaaaaaa cgatatcggc
     4081 ctttaccgct tcaaagatga gtggcagatt ttgccaacaa tacatgaagg ttcatttatt
     4141 ggatctgaag atttatttta cgaaagattt tcagccacaa ctccagggtt ttctagattc
     4201 gccataggcc ttacccttca aactcaagat gctcagtcta tcgcgcctga ctcaagcaaa
     4261 gaaactgctt cccctcaaca acctccagct gattccccta ttcaaccttc aaaacctcaa
     4321 tcagccaacg aatttcctgc tgctacgccc cttactccta cgccccttgc attagttggt
     4381 ttacagacaa ctgaagtccc aacaccagaa cctatgccga cccaatctgt gccaacaccg
     4441 atcctggctg cggtaacgcc tcagacttct gtagaggcat taccaacaca agaaccactc
     4501 cctacagcta ctcccatacc tccaacacaa gtccctaccc caacacgagt ccctccaaca
     4561 tcgactccta ccccgagccc cacacctgtc cttgccgaaa aagtgaaaga cgggtcttta
     4621 attcaattgt cgactaccca acaagctcat atcgaatatc caggggacgt tgatgaatgg
     4681 atatatgttc caagttcctc catgatcgga aagtccgtaa acatttcagg aatcactgat
     4741 aatcctgact cagatgggta tttggaattt ctttcccctt caggggatgt ggttggggaa
     4801 gatgacgaca gcggactcaa catgaatcct gttatcagca acgcattgtt atctgaaagc
     4861 ggggtctaca ccattaggtt catgctatta gacggaacct cagggggtta tacacttcaa
     4921 atagaacctg cggactcaaa tcttctaaca cccactgtca ctctgacacc tacacctaca
     4981 cctacaccta cacctacacc tacacctaca cctacaccta cacctacacc tacacctaca
     5041 cctaccatca ctcctattcc tcccatctct cacacagttt atttcaaaga ttctaatctt
     5101 gaagaagcag ttagaaatag cctaaggaat accataggaa cagcacccga cgaaataact
     5161 tacggggata tggcagaatt aacgaatctt gatgcctatg cagcctcaac aggcgcaata
     5221 actagtcttg ttggcctcga gtttgcaaca aacttagagt ctttgtacct ggataacaac
     5281 tccatatcgg accttggtcc tatttcctcg cttacaaatt tacgttgggt agagttttgg
     5341 ggtaacaata taagtgatct aggtccttta tctgggctaa cgaacctgga atccttgggt
     5401 ctaggcgaaa actccatatc ggacattgga cctatttcct cgcttacaaa cttagagtat
     5461 ttgtacatgg accaaaactc cata
//
LOCUS       DASCNS010000180         7662 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001421235, whole genome shotgun sequence.
ACCESSION   DASCNS010000180 DASCNS010000000
VERSION     DASCNS010000180.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001421235"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="QF838_06445"
     CDS             <1..490
                     /locus_tag="QF838_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60355.1"
                     /translation="AIPIKSLWGGDWTWGPRELDRHITVECAPVMTWNRISIHRSHPI
                     KVKGELVFKGEIYVEGHGASGTPLFSFFLDQMKEGGVFTGEDFESVDVFTSYFQNVIA
                     VRVALPGIVERSVSVLMSGDLKYDEYGLKDYYYLDAVVRSYLQTKGELDHSSFHPDGL
                     GQ"
     gene            complement(492..1325)
                     /locus_tag="QF838_06450"
     CDS             complement(492..1325)
                     /locus_tag="QF838_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO60356.1"
                     /translation="MDYESGVIEVADGVYAWINENCATNAGFIVGDDGVILIDTLMTP
                     TLASKLLTVVKDVTSKPIRFVVNTHFHGDHVYGNQYFLPAPILGHENCRIELDTKWDA
                     NFSRYWTREALRPELERINKTLPDVTFKDQMSIWLGDREIRMSYHGRAHSDSDILLYL
                     PEEKVLFVGDLAVNKTIPAFPDGHISKWVDVLNKVETVDATTFIPGHGPVGGKEEFGE
                     ARDLLKLLHNEIKKAFDDGKNEEQASKDVNVGKFSVFANQDRIPQVVEMAYKAYRGEL
                     D"
     gene            complement(1335..2567)
                     /locus_tag="QF838_06455"
     CDS             complement(1335..2567)
                     /locus_tag="QF838_06455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60357.1"
                     /translation="MANSNKPHGPLSGTRVIDWTMWQFGPVCSMMLADLGAEVIKVES
                     LDGDHARQFHTVSGSSSSLPGGLNAYFESLNRQKKSLSLDLKSPSGQEIMRKLVGESD
                     IFVQNFRKGVAERLGLGYDDLIKINPKLIYGAATGYGPNGPDSDKPAFAYTGEARSGS
                     LWWAGPNDGNPYNLQSIADQISGIMLCNGILGALVNQKETGKGQKVDVSHLGSMMWLG
                     GNRYGMALLTGNEPGGQERKSARNPLWNCYKCADDDWIAFAMNQSDRYWPSFCEAIER
                     PDLNEDERYRNMGVRTENRVELIAKLDDVFISKPRAYWIQRMDSTEGIIWEKVQSIWD
                     LPEDPQVVQNNYIINFEHPALGPVKYLQTPFQYSETPIETKLPAPELGQHTEEILTET
                     LGYTWEDIANFQDQGTII"
     gene            2729..3769
                     /locus_tag="QF838_06460"
     CDS             2729..3769
                     /locus_tag="QF838_06460"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HJO60358.1"
                     /translation="MVKSVLTVIGENIHTTRVLRTNGKRVIRKENGDEYVIYKNIDGI
                     TSFMPIPDSFRDTQVYKQGNVKHFMIAVTLGMSNSDEDRIHGESYISAEIKRQEDRGS
                     NFLDLNVDEISYKIDIQKRAMAWLIGHYSSVAKLPPCIDSSSVEIIQHGLECYRSVGS
                     PQGPPMINSASLERISVLDLVTENNATAIVTASGIDSMPSEISDRISNVSQMIKQCLE
                     REIPLSRIYLDALVFPIAVDQNFGNHYLGAVGQIRERFGEKIHITGGLSNVSFGLPSR
                     KLINDTFIRLSIESGVDAAILNPVESDVERIFALDMESERVQTTINMLLGKDEYCTNF
                     LSKYRSGKLTEI"
     gene            3908..5356
                     /locus_tag="QF838_06465"
     CDS             3908..5356
                     /locus_tag="QF838_06465"
                     /inference="COORDINATES: protein motif:HMM:NF020390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO60359.1"
                     /translation="MTTVHSRQEALLMAHLLRRAGFGATPYEMDRALSMGYQAVLDDL
                     LNPDHPDVIPDDLIRRYHVDQSDLRSGGGAHWVYRLVMTDTPLREKICLFWHRVFATG
                     TTKLIQNRVVTNQIDMFREHGMGRFDDLLVELSRDPAMIMWLDNQDNHGSNINENYGR
                     EILELFSMGVGNYSEDDIKETARAFTGWRVVNPDYMSIRMRNNTARPYGYMSWQFEYD
                     DDDHDDGEKIILGQKGKWNGEDAVRIICEQDATARFVARHLYHFFVADELPVPQWPHE
                     EPRDPEAIDVLCKSYFENNHDIGAMLRTLFESDFFKDAGSRYARIKSPSEMVIGTMRL
                     AGPIDLPSEETYMADAACANMGQALMRPPSVEGWQGGTEWINTGAYVERVNFAGRILN
                     DSDKQGVRDIIDRIKALSDRQTITSDELVDNCLEVLGPLEVLDTTRHGLKNYASKFGD
                     LKWSDQSLSSEFDRAAVAIIQLVVSSQEYQTI"
     gene            5375..6373
                     /locus_tag="QF838_06470"
     CDS             5375..6373
                     /locus_tag="QF838_06470"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HJO60360.1"
                     /translation="MTTTTFGLKYVTNVGFAADFGGRGFQLPSSMAIRADGVIFVVSR
                     GKSSTKNSNGIQMVTKNHDFLGQIGTYGAGLGGMMWPTSVVLDSDENLYLADEYFNKV
                     TKYDREGNPLTEWGTKGSGNGQFNQPSGLLIQDQLIYLVDSRNNRIQMYSLDGDFQGQ
                     WGSEGDGEGEFNIPWGISDDSEGNIYVADWRNDRIQKFDSNGNYISAIGEGDTSVLDR
                     PSDVAVDPDGNIYVTDWGSQRLLVLDQSGSILETKRGEADLNPWSMEYLSSQDDERKA
                     RESFIPVYKADTEDPSEVSARMEPYFWDPVAVELDQDGTVYVLETGRHRFQIFERI"
     gene            6413..7534
                     /locus_tag="QF838_06475"
     CDS             6413..7534
                     /locus_tag="QF838_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HJO60361.1"
                     /translation="MGEKKIVVVQLSGGNDYLNCVIPYDNPHYVDNRPNVRITEDRVI
                     PIGDGLGFNPVMSLIKDLYDQGKVAVIHGVGYPEPNRSHFRSMDIWHTAEPTKVGNEG
                     WLGNAINQMYPKHDNVVAAVNFGAGLPRALVSHGAPVASVTDLNTYGLMNHVSELDQR
                     NEALQAFKDMYSQAIGSGPVMDYLSQTGLDALKGAEIIGGVTDKYSSDVEYAENSFAK
                     SMKGAAQILQADIGTRICYTQHGSFDAHTNGIALQEGLLTDVSGGIYDFYDDMKRANK
                     SDDVIMFVFTEFGRRVKDNGNGTDHGSGGVAFVIGDQVDGGQYSEYPSLAADQLLEGD
                     LRFNLDFRSVYTEILEDWLEIDASSVVKGEYEKVGFLKN"
BASE COUNT         2211 a         1479 c         1738 g         2234 t
ORIGIN      
        1 agctatacca ataaaaagtc tgtggggtgg cgactggacc tggggtccgc gggagttgga
       61 cagacatatc actgttgaat gtgcacctgt aatgacatgg aacagaatat cgattcatcg
      121 ttcccaccct attaaagtaa agggggaact agtttttaaa ggagagattt atgtcgaagg
      181 gcatggagct tctggcaccc cgttattttc cttcttccta gaccaaatga aagagggtgg
      241 agtatttaca ggagaagatt tcgaatcagt cgatgtcttt acttcttact tccagaatgt
      301 cattgctgtt cgagttgctc tgccaggcat tgtggagaga agtgtttcgg ttttaatgag
      361 cggtgatctt aagtacgatg agtacggttt gaaggattat tattatttag atgcagtggt
      421 aagaagctat cttcagacga aaggagagct cgaccactca agctttcacc ccgacgggtt
      481 aggacagtaa tctaatccaa ctcaccccgg tatgctttgt atgccatttc cactacttga
      541 ggtattctgt cttggttggc aaaaactgag aattttccga catttacatc tttggatgcc
      601 tgttcttcat ttttcccatc atcaaaggct ttttttattt cattatgcaa caatttcaaa
      661 aggtcacggg cttctccaaa ctcctctttt cctcctactg gtccatgtcc gggaatgaaa
      721 gtggtagcat caacagtttc caccttgttt aatacatcca cccatttaga aatatggccg
      781 tctgggaagg caggaatggt tttatttaca gccaggtctc ccacaaacaa cactttttcc
      841 tcaggtaaat ataataaaat atcactgtct gaatgtgccc gcccgtggta ggacattctt
      901 atttctcgat cccctaacca aatagacatt tgatctttga atgttacatc ggggagtgtt
      961 ttgttaatcc gttccaactc tggtcttaat gcctctcttg tccaatacct agaaaaatta
     1021 gcatcccatt ttgtgtcgag ctcaattctg caattttcat gtcctaggat tggagcgggt
     1081 agaaaatatt ggttaccata tacgtggtct ccatgaaaat gtgtgttcac aacaaatcta
     1141 attggtttag aagtaacatc tttcacaaca gttagaagct tagacgctag cgtgggtgtc
     1201 attaaagtgt ctataagtat tacaccgtca tcgccaacga tgaatccagc attagttgca
     1261 cagttttcgt ttatccatgc gtacaccccg tctgctacct ctattacacc tgattcgtaa
     1321 tccatactct ctccctaaat tatggttcct tgatcttgga aatttgcaat atcttcccat
     1381 gtatacccga gtgtttcagt taatatttct tcagtatgtt gccccaattc cggggcaggc
     1441 aattttgtct ctattggagt ctcgctatac tgaaacggag tttgaagata ttttactgga
     1501 cctaaggctg gatgttcaaa atttatgatg taattattct gtacgacttg gggatcttct
     1561 ggaagatccc atattgactg aactttttcc catattattc cttcagttga atccatcctt
     1621 tgaatccaat atgcccttgg tttggatatg aacacatcgt ctagcttcgc aattaattcg
     1681 accctatttt cagttctaac tcccatattg cggtatcgtt catcttcgtt caaatcgggt
     1741 ctttcaattg cttcacaaaa tgatggccag tatctatcac tttggttcat agcaaatgcg
     1801 atccagtcat catcggcaca tttgtaacag ttccacagag ggtttctagc actctttcgt
     1861 tcttggcctc ctggttcgtt cccagttagt aaggccatcc cataacggtt gcctcccaac
     1921 cacatcatac ttccgagatg agaaacatca actttttgcc ctttacctgt ctctttttga
     1981 ttaactaacg cacccaaaat cccattacat aacatgatcc ctgaaatttg gtcagcgata
     2041 ctttgtaaat tatatgggtt accatcattt ggaccggccc accacaagga gcccgatctg
     2101 gcctcacccg tataagcgaa tgcgggttta tcagagtcag gcccgtttgg accatatcca
     2161 gtggctgcac catatattaa tttagggttg atcttaatta gatcgtcata acctaacccc
     2221 aatctttcag ctactccttt tctgaaattt tgaacgaata tgtccgattc acctactagt
     2281 ttccgcataa tctcttgacc agagggactt tttaaatcta atgaaagact tttcttttga
     2341 cggttgaggg attcgaaata tgcattcaag cctccaggga gtgaggagga ggacccgctg
     2401 actgtgtgaa attgtcgtgc atggtcaccg tcaagagatt caactttaat aacctctgca
     2461 cctaggtccg ccaacatcat cgaacatacc ggcccaaact gccacattgt ccagtcaatt
     2521 acacgcgttc ctgataaagg gccgtgaggt ttgtttgaat ttgccatatc catttgtacc
     2581 tatgtttatt cagggtttgg aatatttaag cagtagtatg cctggcggag tctattgggt
     2641 atgtgtttcg cagtcaaatt gtatttcctg atggagcaga aaaaattatt actttctatt
     2701 aaaatggtca atatagttgg aggaattaat ggttaaatcg gttttaacgg tcattgggga
     2761 aaatattcat acaacccgtg tcttacgcac caacggtaaa agggtcatca gaaaagaaaa
     2821 tggagatgaa tatgtcattt ataagaacat agatggcatc acttccttta tgccgatacc
     2881 tgattctttc agagacactc aagtttacaa gcaaggaaac gtaaagcatt tcatgatagc
     2941 cgtaactctt ggtatgtcta attcagatga agataggatt catggcgaga gctacatctc
     3001 agcagaaata aaacgtcaag aagatagagg tagtaatttt ctagatttaa atgttgacga
     3061 gatctcttac aaaattgata ttcaaaaaag ggcgatggca tggttaattg gccactattc
     3121 gtcagtcgca aaattaccgc catgtataga ttcttctagt gtcgaaataa ttcagcacgg
     3181 cttagaatgt tatcggagtg ttggttcacc gcaaggtccc cctatgataa attcagcttc
     3241 attagaaagg atttcagttt tagatcttgt tactgaaaat aatgcgacag cgattgtaac
     3301 ggcgagtggt atcgattcta tgcctagtga aatctcagac cgcatatcca atgtgtccca
     3361 gatgataaaa caatgtttgg aaagagaaat ccccctatca cgaatctact tggatgctct
     3421 ggtattccct atagccgtag atcagaactt cggcaatcat tatcttggag cagttggcca
     3481 aatccgcgaa cgatttggag agaaaattca tataactgga ggattgagta acgtctcttt
     3541 tgggcttcca tcgagaaaat taattaatga cactttcatt agactttcga ttgagtcggg
     3601 ggtagatgca gctatattga atcctgttga aagtgatgtg gaaagaatat tcgccctaga
     3661 tatggaatct gaacgggtac aaaccacaat taatatgctt ttaggcaaag atgaatattg
     3721 cacgaatttt cttagtaagt ataggtcggg taaactcacc gagatttagt attggttcaa
     3781 gtcgtttttg cgtgcctgca tataaaaata tatatggctt ttgacatgcg caacccgctt
     3841 ggtatactca tgccaataaa aaactcacat ttcaagaaaa cacactgaga tcgggggagg
     3901 caaaaatatg acaacagttc attctaggca agaggcattg ttaatggctc accttctgag
     3961 aagggctgga tttggagcaa ctccatatga aatggatcgg gctcttagca tgggatatca
     4021 agctgttctt gatgatttgt tgaacccgga ccaccccgat gtcataccgg atgatttgat
     4081 aaggagatac cacgttgacc agtctgactt acgcagtggc ggaggtgcgc attgggtata
     4141 taggctagtc atgactgata ctccattgcg agaaaaaatt tgtctttttt ggcatagggt
     4201 gtttgccact ggtacaacca aactcattca aaacagagtc gttacaaatc agatagatat
     4261 gtttagagaa catggaatgg ggcggttcga tgatcttctt gtagaactgt ctagagaccc
     4321 agcaatgatt atgtggttgg acaatcagga caaccacggg tccaatatta atgaaaacta
     4381 cggtagggaa attttagaat tattctcaat gggagttggc aactactctg aagatgatat
     4441 aaaagagact gcgagagcct tcacaggatg gagagttgtt aaccccgatt atatgtccat
     4501 cagaatgcga aataatacag ctagacccta cggatacatg tcctggcaat ttgaatacga
     4561 tgacgatgat catgacgatg gagaaaagat aattctgggt caaaaaggaa aatggaacgg
     4621 ggaagatgct gtcagaataa tttgcgaaca ggatgcgacg gctagatttg tagctcggca
     4681 tctatatcat tttttcgttg ctgacgaatt gcctgtgcca cagtggccgc atgaagagcc
     4741 aagagatcca gaagctatcg atgttttatg taaatcttat tttgaaaaca accatgatat
     4801 cggagcaatg ctgagaacct tatttgaatc tgattttttc aaagatgctg ggtcaagata
     4861 cgctcgaata aagagccctt cggagatggt cataggcaca atgcgtttgg ctggtccaat
     4921 tgatcttcct tctgaggaaa cttacatggc ggatgctgca tgcgccaaca tgggacaggc
     4981 attgatgcgt ccaccaagtg tggagggatg gcaaggggga actgaatgga ttaataccgg
     5041 tgcctatgtc gaaagggtca atttcgctgg gcgtattttg aatgattctg acaagcaagg
     5101 tgttcgcgat atcatagatc gaataaaggc attgtcggac cgacaaacca ttacatcaga
     5161 tgaactagtt gataattgcc tagaggtttt agggcctttg gaggtacttg atacaactcg
     5221 tcacggttta aaaaactacg cttctaaatt tggtgatttg aaatggtcag atcagtctct
     5281 aagtagtgaa tttgatcgtg cggctgttgc tattattcaa ctcgttgtat caagtcaaga
     5341 atatcaaact atttaaggta ttgagaggaa aaacatgact acaactacct ttggattgaa
     5401 atatgtgact aatgttggat tcgctgccga tttcggaggc agaggttttc aactaccttc
     5461 atcaatggcg ataagagccg atggggtcat atttgttgtc agtcgaggaa aatcctctac
     5521 caaaaactcc aatggtatac agatggttac taagaaccac gacttcctag gccagatcgg
     5581 tacttatgga gctggcctag gaggaatgat gtggccaact tctgtggttc tcgatagtga
     5641 tgaaaatctg tatcttgcgg acgaatattt taataaagtg accaaatatg atcgtgaagg
     5701 taacccactc acagaatggg gaactaaagg ttctggtaac ggacaattta atcaaccgag
     5761 tgggttattg attcaggatc aactgatata tttggttgat tcaagaaaca acagaattca
     5821 aatgtacagc ttggatggag acttccaagg tcagtggggc tcagaaggtg atggggaagg
     5881 agaattcaat ataccctggg gtatatctga cgactctgag ggtaatattt atgtcgctga
     5941 ctggcgtaac gaccgaattc aaaaattcga ttcaaatggg aattacatat ctgctattgg
     6001 ggagggggat acctctgttc tagatcgacc ttctgatgtt gccgtggatc cagatgggaa
     6061 tatttatgtc acagattggg gcagtcagcg tctattggtt ttagaccaat cgggcagcat
     6121 tttggaaact aaaagaggtg aagctgattt aaatccgtgg tcaatggaat atttgtcctc
     6181 ccaagacgat gaaaggaaag caagagaatc atttattcca gtctacaaag ctgataccga
     6241 ggatccttct gaggtgtctg ccagaatgga accctatttc tgggatcctg ttgcagtaga
     6301 actggaccaa gatggaacgg tgtatgtact tgagaccggt aggcaccgat ttcaaatttt
     6361 cgaaagaatt taatagaatg gtcaacatac atatagggaa gaggagaaca atatgggtga
     6421 aaaaaaaata gtcgttgtcc agttatcggg aggtaacgat taccttaatt gtgtgattcc
     6481 atatgacaat ccacattatg tggacaatcg tcccaatgtg cggataacag aggatagggt
     6541 gataccaata ggagatggct taggctttaa cccggttatg agccttatta aggatttgta
     6601 tgatcaaggt aaagttgcgg tgattcacgg ggtgggatat cccgagccaa acagatctca
     6661 ctttaggtct atggatattt ggcacaccgc agaaccaacc aaagttggta atgaaggctg
     6721 gttagggaat gctataaacc aaatgtatcc aaagcatgac aatgtggttg cagccgttaa
     6781 tttcggtgct ggactaccac gggcactggt atcccatgga gcccctgtgg catcggtgac
     6841 agatttgaac acttatggcc ttatgaacca tgtcagtgag ctagaccaac gaaatgaggc
     6901 ccttcaggca tttaaagaca tgtactcgca agcaattggg agtggtccgg ttatggacta
     6961 cctaagccag acgggccttg atgctttgaa aggtgcagag ataataggtg gtgtgaccga
     7021 taagtactcc tcagatgttg aatacgctga aaattcgttt gcaaaatcaa tgaaaggagc
     7081 agcccaaatt ttacaggctg atattggcac taggatttgc tatacccagc atggtagctt
     7141 cgacgcccac acgaatggga ttgcccttca agaaggttta ttgaccgatg tgtctggtgg
     7201 aatatacgat ttttatgacg atatgaagag ggcaaataaa agtgacgatg taattatgtt
     7261 tgttttcaca gaatttggaa gaagggtcaa agacaatggc aatggtacag atcatgggtc
     7321 tggaggtgtt gcgtttgtca ttggggatca ggtagacgga ggtcagtaca gtgaataccc
     7381 ttccctcgcc gctgaccaac tcctggaagg cgatttgaga tttaatttgg actttcggag
     7441 cgtatatacg gagattttag aagattggtt ggaaattgat gctagttcag tggttaaagg
     7501 cgaatatgag aaggtcggat ttctaaaaaa ctagataatc ccacatcagg caaaaagccc
     7561 gcaggttaat aaattaacct gcgggctttt tgcctgatgt gggattatct agttttttag
     7621 aaatccgacc ttctcatctt cgcctttaac cactgtacta gc
//
LOCUS       DASCNS010000181         2280 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001435119, whole genome shotgun sequence.
ACCESSION   DASCNS010000181 DASCNS010000000
VERSION     DASCNS010000181.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001435119"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            49..681
                     /locus_tag="QF838_06480"
     CDS             49..681
                     /locus_tag="QF838_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HJO60362.1"
                     /translation="MKNVFVQMQNLYSDRKGITGLETAIILIAFIVVAAVFAFTVMTT
                     GLFSTEKAKTTAQAGIAEASSTFAPKGGVIAASNAAKNAVETITFQITTASGSMGTSI
                     AKAKIAMIYSDDNQRDSSSANITNRGTADGIMKSVAISKIVGDATSPDLLQQGDVAEI
                     TVTLNTTTAGKATLLDKNQIFRLELIPQQGGSLIISRKTPPELMKVMNLE"
     gene            693..1220
                     /locus_tag="QF838_06485"
     CDS             693..1220
                     /locus_tag="QF838_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60363.1"
                     /translation="MTTSNSQLEQRIQALEDEVNILKTEIRQILIDMRDTWLRKSDVL
                     ATSREIVQAAPTLAPTPQPATPAANGAPRTYFGSEAGLDLSADQNNKGTLNDLLAWMG
                     EAKEKGLTAARLFPILEAYENSAMISPLMSKFILKNMSMLDEMYSNNDPMSPAVYSQV
                     VSELHKLVCISIETS"
     gene            1266..1784
                     /locus_tag="QF838_06490"
     CDS             1266..1784
                     /locus_tag="QF838_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60364.1"
                     /translation="MDKVIVTALLVIAGVTAAGLVMTTLTPIVGSSSQSVVESQKDLS
                     RRIQTDFEITAVHADSDVLVRAWIKNVGGANISFIEQSDVFVSSTDKEQFVFLCYDEP
                     ISCTENKWSAFEKDGVTKKTGYWTEGETIQLTLSLKDDAISKLVQSTRTYNFEFTTSN
                     GVKSAYQFKYGQ"
     gene            1819..>2280
                     /locus_tag="QF838_06495"
     CDS             1819..>2280
                     /locus_tag="QF838_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60365.1"
                     /translation="MGTSISALLIISVLLTAVVVMFKANQIGAELLAVANKSTAEIIA
                     DRVSTKIEISSTQDFKDSYEEEEKHHLQDTEGCRPILPGIPRSGDITTTNSLDCYWFQ
                     ADSQAQATLTVDKVIGSPVSLANRTGTNTKISIDYFPPGEVITATNASSKFK"
BASE COUNT          692 a          451 c          535 g          602 t
ORIGIN      
        1 tcattttgag atgggggagt tacatatagt tagtattggg gatatgttat gaagaatgtc
       61 tttgttcaaa tgcagaatct ctattccgac aggaaaggga ttacgggcct tgaaacagcg
      121 atcatattaa tagcttttat cgtggttgcg gctgtctttg cttttaccgt catgactacc
      181 ggattattca gcactgagaa agctaaaact actgcccagg ctggtatcgc tgaggcaagc
      241 tctacattcg ccccgaaagg tggggttatt gctgcttcta atgcggcaaa aaatgcagtg
      301 gaaacgatta cgttccaaat tactactgct agtggatcga tgggaacttc aatagcgaaa
      361 gcaaaaatag ccatgattta ttccgatgat aatcaaagag actcttccag tgcgaatata
      421 acgaacagag gaactgctga tgggataatg aaatcggtgg cgataagtaa aattgtcggg
      481 gacgcgacct caccggatct gctgcagcag ggtgatgttg cagaaatcac tgttactctc
      541 aacactacta cggcgggcaa agccacacta cttgataaaa accagatttt tagacttgaa
      601 ttaatccctc aacaaggtgg gtccttaatt atttcaagaa aaactccacc ggagttgatg
      661 aaagtgatga atctggaata attggtgaca aaatgacaac atctaattca cagttagagc
      721 agagaattca ggctttagaa gatgaagtca atattttaaa aactgaaatt cgtcaaatat
      781 tgatcgatat gcgggatact tggcttcgga agtcagatgt actggcgact tctcgagaga
      841 tagtgcaagc ggcaccaacg ctggctccga caccacaacc cgctactcca gcagcaaacg
      901 gggcgcctcg cacatatttt ggctctgaag ccggattgga tttgtcagcc gaccaaaaca
      961 ataaaggtac cctgaatgat ttgctcgctt ggatgggaga agctaaggaa aaaggcctta
     1021 ctgcagctag actgtttcct atcctggagg catatgaaaa ttcggccatg ataagccctt
     1081 tgatgtcgaa atttattctg aagaacatgt caatgcttga cgaaatgtat tcaaacaatg
     1141 acccgatgtc accggctgtt tactcacagg tcgtatcgga acttcataag ctagtttgta
     1201 tatccataga aacctcatag gggtatacct ggctttccta gactttgatt taaagggaaa
     1261 atagattgga taaggtcata gtcacagctc tgttggtaat agcaggtgtc actgcggcag
     1321 gtttggtgat gactactcta acgccgatag ttggtagcag tagccagtca gtcgttgaat
     1381 cacaaaagga cctttctcgg cggatacaaa ccgattttga gataacggcg gttcacgccg
     1441 atagtgatgt tttggtcagg gcctggataa agaatgtggg tggggcaaat atcagtttca
     1501 tagaacaatc agatgtgttt gtgtcatcaa ctgataaaga gcagtttgtg ttcctatgtt
     1561 acgacgaacc aataagctgt acggaaaaca aatggtccgc ttttgaaaag gatggggtga
     1621 ccaaaaagac cgggtattgg acagagggag aaacaattca attaaccctc tctcttaagg
     1681 acgacgccat tagcaagtta gttcagagta ctagaacata caatttcgaa tttactactt
     1741 ctaacggggt caaatctgct taccagttca aatacggcca atagaggtct acctagatgg
     1801 actggatcct aatttgatat gggaacaagt atttctgcat tattgatcat ttcggtactt
     1861 ctaacagcgg ttgtcgtcat gttcaaggcc aaccaaatag gggctgagct gctagctgtc
     1921 gccaacaaaa gtacagcgga gataattgca gatagagtta gcacaaagat agaaatttca
     1981 agtacacagg atttcaagga cagttatgaa gaagaggaaa aacatcacct ccaagacact
     2041 gaaggatgtc gcccgatatt gccaggtatc ccgcggagtg gagatatcac tactaccaat
     2101 tccttggatt gttactggtt ccaggccgat tctcaagcac aagcgacttt gactgtagac
     2161 aaggtaattg gaagtcctgt ttctcttgcc aaccgtactg ggactaatac caagatttcc
     2221 attgattatt ttccacctgg tgaggtgatt acggcgacta atgcatcttc taaattcaag
//
LOCUS       DASCNS010000182         6988 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001437491, whole genome shotgun sequence.
ACCESSION   DASCNS010000182 DASCNS010000000
VERSION     DASCNS010000182.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001437491"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="QF838_06500"
     CDS             <1..507
                     /locus_tag="QF838_06500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJO60366.1"
                     /translation="SSTLTRAIQTTENIFTGMGNPNNSFQKIPEIIEQQIPGWRGVPT
                     EEAYTNDTMTYIMEKGRDFVGPDGESIREVQKRATNWLEDELIYNRKLCSTPTSLKIA
                     IIGHGNTMRSIFHYIMGFDQRLIYKIGVDNTSISRFIFNSKGWSVVCLNDNSHLRTGP
                     SGTFEARS"
     gene            607..1641
                     /locus_tag="QF838_06505"
     CDS             607..1641
                     /locus_tag="QF838_06505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60367.1"
                     /translation="MNFGSFMEFHTREGNGQEDAFQESFDHVALAEELGLDTVWLAET
                     HFNPNRSVLSAPLVLAAAIAGRTKKIKIGTAVQILPLGNPLRIAEEVATLDHVSGGRF
                     EFGIGRSGLPSAYNGYNIPYTESRERFYEAMEIIKKAFTEDSFTYSGKYNSYEDVCLT
                     PKPLQNPYPQMRIAATTSETFTTLGQMGIPIFVGVRRLSLYDVAQQVVLYRDALRESE
                     HATGSILLRVPVHVANSTQEALANSEASFMKQFRRLGSQLGTNATASLNTEGNTSKNL
                     SEIDWTDIQGEKVAVGDPDTVVEQLKNMIDVLTLDGIAAEFNAGEEIPAGIVENSLSL
                     FCKEVMPKLR"
     gene            complement(1909..2394)
                     /locus_tag="QF838_06510"
     CDS             complement(1909..2394)
                     /locus_tag="QF838_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HJO60368.1"
                     /translation="MPSFDIVSKTDLMEVDNAVNGVKRQITQRFDLAGTKCDIARSEN
                     TLTITADDNMKLTQLHDLLRQNFASRKVDAKALDFGKAENASGDSLRQVISIKQGIDA
                     DLARKINKSVKGSKMKVQISIKGSELHVSGKKRDELQEAIAFIKKMETNQPLQYVNFR
                     D"
     gene            complement(2437..3393)
                     /locus_tag="QF838_06515"
     CDS             complement(2437..3393)
                     /locus_tag="QF838_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HJO60369.1"
                     /translation="MLNVAVLDDYQNVAVGFANWGQLSSEAKVTVFNDHIHDSDSLVK
                     RLESFEIVCAMRERTPFPIEILQRLPKLQLLITTGMRNASIDVKEANNLGVVVCGTDG
                     LLYPTAELAWGLILDLARNIAKENESVKRGKWQSTVGVGLKGKTLGLIGLGNLGGQVA
                     NYGNAFGMDVIAWSQNLTKEKAERRNTKYVSLETLMAESDFISIHTVLSSRTKDLIDA
                     SKLKLMKKTSFLINTSRGPIVNELALVNALAEGEIAGAALDVFSLEPLPSDHPLITLE
                     NTIITPHIGYVTNETYKIFYEQTVECVIGFIQGNPIRVISPI"
     gene            complement(3386..4096)
                     /locus_tag="QF838_06520"
     CDS             complement(3386..4096)
                     /locus_tag="QF838_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HJO60370.1"
                     /translation="MSENKDLFSTVCPGVLTICTYTEFAPFSYVDGGDIVGTDIGLLK
                     AFAREIQLEVEVIKRAFAGLWEAPGKGDCDVSAAGMMEHEDRKLGKNAVWSYPYMLVT
                     RSLLIRKSDRDILKGPKDFQGRKIVVTPTSSAHIDGSIRYEPLGATIIPLVPSQDEIV
                     SQLLSGEVDAFGEGDVSNEYLAGKYLDDYGRELLVLTDIHPMENPELLRFAVRSVDER
                     LPDKLNGFIAKTQRGINA"
     gene            complement(4128..4976)
                     /locus_tag="QF838_06525"
     CDS             complement(4128..4976)
                     /locus_tag="QF838_06525"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HJO60371.1"
                     /translation="MAETTKKLVSGSWLLDHLYDLDVTVLEVSSDQEGKAYENGHIPG
                     AIFCFWKDLLWHETDREFATSSELSDRLGDLGISTDSTIVLCGDPVQYGTYALWVMIM
                     AGVKDVRILDGSRLRWIKDNHPLTSEVPTLKPIKNKTPKPNSESRIGRVEVLDKLNSS
                     NNIFLDARSPEEYRGERVSPPGGFDHGAERRGRIPGAVHLFFRDLLSEDDTFISEEDL
                     KKKFAKVGITGDGEKEIVSYCRLSHRATLAWFAINEILGIDKVKIYDGSWTEWGSIVG
                     FPVEQG"
     gene            5142..6196
                     /locus_tag="QF838_06530"
                     /pseudo
     CDS             5142..6196
                     /locus_tag="QF838_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            complement(6197..6529)
                     /locus_tag="QF838_06535"
     CDS             complement(6197..6529)
                     /locus_tag="QF838_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60372.1"
                     /translation="MERLRPFHDERFKWFAPFGFVRYADEEGRVWGTPGAPARTPKIE
                     DGVEQKAWICGPPSHQIKFLKEVEAKYPGLENIILHWPEGMPRDEYLSQLRIFAEEVM
                     PAFGGRQE"
     gene            complement(6554..>6988)
                     /locus_tag="QF838_06540"
     CDS             complement(6554..>6988)
                     /locus_tag="QF838_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60373.1"
                     /translation="RMIFGVGRGYHSREVETFGSPVIDNDLNRELFEEQIEVILKAFN
                     SESFSHHGKNYSIPAPVPYRGYDLEEITLVPKPVNLPVETWQPIVSGGSIDFIARNGF
                     KGLVALTGEKLVDEVFHRFRDACSENGRGNVLGSNLALGIGF"
BASE COUNT         2001 a         1621 c         1343 g         2023 t
ORIGIN      
        1 agttcaactt tgactagagc tattcaaaca acagaaaaca ttttcacggg tatgggtaat
       61 ccaaacaata gcttccagaa aattccagag ataatagaac aacaaattcc agggtggcgc
      121 ggcgtcccaa ctgaggaggc ctataccaac gatacaatga cttacatcat ggaaaagggt
      181 agggactttg tagggcccga tggcgaaagc ataagggaag tgcaaaaaag agcaacgaat
      241 tggctagaag atgaactgat ttacaaccgc aaactctgta gcacccctac atccttgaag
      301 atagctatca taggccatgg gaatactatg aggtcaatat ttcattacat catgggtttt
      361 gatcaaaggt tgatatataa aattggtgta gataacactt caatttccag gtttatattt
      421 aacagcaagg gatggtccgt tgtttgcctg aatgacaact cccatttaag aactggccct
      481 tctggaacgt ttgaggctag aagttgaatt cccgccttct acccttgatt tctacaccta
      541 gcctctttat tctgatgcca atttcagaaa attgttgact cccttttggt tcacaaagga
      601 taaagaatga attttggaag cttcatggaa ttccatacta gagaaggaaa tggacaagag
      661 gacgcttttc aagaatcctt tgaccatgtc gcactcgctg aagaactggg gctcgataca
      721 gtctggctag ccgaaaccca cttcaaccca aaccgttcag tgttatctgc tccgttagtt
      781 ttagcagcag cgatagcggg ccgaacaaaa aaaatcaaaa taggcacagc agtacaaata
      841 ttaccgcttg gtaaccctct cagaatcgcc gaagaagtag caaccttaga ccatgtcagt
      901 ggaggacgat tcgaatttgg tatcggtagg agtgggcttc ctagtgccta taatgggtac
      961 aacataccat acacggaaag cagagagcga ttttatgagg ccatggagat aatcaaaaag
     1021 gctttcactg aagacagctt cacttattcc gggaaatata acagttatga agacgtgtgt
     1081 ttgaccccaa aaccgttgca aaatccgtac ccgcaaatgc gtatagcagc cacaacctcc
     1141 gaaactttca ccacattggg gcagatggga attccaattt ttgttggagt aagaaggctt
     1201 tctttatacg acgttgccca gcaagtcgtt ttgtatcgtg atgcccttag agaatccgaa
     1261 catgctactg gtagtatttt gctaagggtt ccagtgcacg tagcaaactc gacccaggaa
     1321 gctttggcaa attcggaagc gagtttcatg aaacagttcc gacgcttggg atctcagctt
     1381 ggtactaatg cgacggcatc attaaatact gaaggcaata ccagcaaaaa cctatcggaa
     1441 atagactgga ccgatataca gggggaaaag gtagcagtag gagacccgga taccgttgtg
     1501 gaacaactca aaaatatgat tgatgttctt actctagacg ggattgcagc agagtttaat
     1561 gcgggagaag aaattcctgc cggcattgta gaaaactcgc tttcactatt ttgtaaagaa
     1621 gtaatgccaa agttgcgttg aaaaatacta ttctaggaaa tttttcgact ggcacgttct
     1681 ttcaatgaga tcagcttcta ccttcataat aggctcagtt attttgatca tatatctgct
     1741 atatcgattt cttgtcgctg cagattaagg gaaattagat gtccagcaac gattacgttt
     1801 ccttggttcc gaaatacaca ttctcatcag cacttaaaga acaaaaatca gacctgaaac
     1861 acaatcccct cacggcctgt atggcagatg caaaatatct aggcgtaatc agtccctaaa
     1921 gtttacatac tgtagcggct gattagtttc catttttttt ataaaagcta tggcttcttg
     1981 aagttcatca cgtttcttac ctgacacatg aagttcactt ccctttatag aaatctgaac
     2041 cttcattttg gatcctttca cagatttgtt aatctttcta gcaaggtcag catcaattcc
     2101 ttgcttaata gatattactt gtcttagtga gtcccccgaa gcattttccg cctttccaaa
     2161 gtccagtgcc ttagcatcaa cttttctact ggcaaaattt tgcctcaata ggtcgtgtaa
     2221 ttgcgtcagc ttcatattgt cgtcggcagt tatagttaat gtgttttcgc ttcgtgctat
     2281 gtcacattta gttcccgcaa gatcaaaccg ttgtgtaatc tgacgtttta ctccattaac
     2341 agcattatcg acttccataa ggtctgtttt tgaaacaata tcgaatgatg gcatttgcta
     2401 tttcctccat aaacttgccc ttaaccttta gttaaatcag atagggctta ttactctaat
     2461 agggtttcct tgaataaatc cgataacaca ttctactgtc tgttcgtaga aaatcttata
     2521 cgtttcattt gtaacatatc caatatgtgg ggtaataatg gtgttttcta aagtgattaa
     2581 aggatgatct gaaggcaatg gctccaagct aaacacatcc aatgcagcac cagctatttc
     2641 accttcggcc aaggcattaa ctagtgctaa ttcattcacg attgggcctc gagaggtatt
     2701 gataagaaaa ctagtttttt tcatcaattt aagtttagat gcatcaataa ggtctttagt
     2761 ccttgagcta agcacagtgt ggatactgat aaaatctgat tcagccatta gggtttccaa
     2821 agaaacatat ttcgtattcc ttctttctgc tttttcttta gttaaatttt ggctccaagc
     2881 gatcacatcc atcccaaaag catttccata attagccacc tggccaccaa gattacctag
     2941 accaatcagc ccgagggttt ttcccttcaa tccaacacct accgtacttt gccactttcc
     3001 ccttttaact gactcattct cctttgctat gtttcgagct aaatctaaaa tcaaccccca
     3061 ggctaattca gctgtcgggt agagaaggcc atctgttcca caaacgacga cacccaggtt
     3121 attcgcttct ttaacatcta ttgatgcgtt cctcataccg gtcgtaatca ataattgcaa
     3181 tttagggagt cgctgtaata tttcgatagg gaaaggagtt ctttctctca tagcacaaac
     3241 gatttcgaac gattccaacc ttttcactaa agaatccgaa tcgtggatgt gatcattgaa
     3301 aactgtaacc ttagcttcgc tcgatagttg accccagttt gcgaatccaa ccgcaacatt
     3361 ctgatagtca tccaacacag ccacattaag catttatacc cctttgagtc ttcgctataa
     3421 acccgttcag tttatctgga agtcgttcat caacggatct tacagcaaac ctcagaagtt
     3481 ccggattttc cataggatga atgtctgtca aaacaagtaa ttccctccca taatcatcaa
     3541 ggtatttccc cgcaagatat tcattactga catcaccttc cccaaaagcg tctacctctc
     3601 ctgacaacaa ttgtgacacg atttcatctt gggaaggaac aagcggaatt atcgttgctc
     3661 ctagcggttc atacctgata ctcccatcta tatgagcact tgaggtggga gttacaacta
     3721 tcttcctacc ttgaaaatcc ttcgggccct taagaatgtc cctatcagac ttacgaatca
     3781 acaatgatct agtgacaagc atgtaaggat agctccatac cgcatttttc ccgagttttc
     3841 gatcctcatg ttccatcatc cctgctgccg atacatcaca atcacccttt ccgggtgctt
     3901 cccataatcc cgcgaaggct ctctttataa cctccacttc tagctgtatt tcccgtgcaa
     3961 atgccttcaa caaaccgata tcggtaccta caatatctcc accatctaca tacgaaaaag
     4021 gagcaaactc agtataagta catattgtaa gtacacctgg acatacagtt gaaaatagat
     4081 ctttattttc tgacaaaaca taaacccctt tgctgaagtt agaaaattta tccctgttcc
     4141 accggaaacc caactatgct tccccattct gtccaggagc catcatagat cttcacttta
     4201 tctattccta gaatttcatt gatagcaaac cacgccaaag tggcccggtg gctcagccta
     4261 caataactca caatttcttt ttctccatcc ccagtaatgc ccactttggc gaatttcttt
     4321 ttaaggtcct cttccgatat aaacgtatca tcttcactta ataagtcgcg gaagaacagg
     4381 tgcactgcgc cagggattcg ccctcttctt tctgcaccat gatcgaatcc tcccggaggg
     4441 ctaactcttt ctccccgata ttcttccgga gaacgggcgt ctaaaaatat attgtttgag
     4501 gaatttagtt tatctagaac ctcaactctt cctatcctac tttcagaatt gggttttggg
     4561 gttttattct taataggttt gagtgttgga acctcagatg tcaaaggatg gttgtcttta
     4621 atccatcgta acctgctacc gtccaaaatt ctcacatcct ttactcctgc cataatcatt
     4681 acccagagag cgtatgttcc atattgaact gggtcaccac aaagcactat cgtggaatca
     4741 gtagaaatac ccaaatcacc tagtctgtcg ctaagctcag aagatgtggc aaattctctg
     4801 tcagtctcat gccatagaag atctttccaa aaacaaaata ttgctccggg tatatgacca
     4861 ttttcatacg ctttcccttc ctggtctgac gacacttcca gcacggtaac atcaaggtcg
     4921 tagaggtggt ctaatagcca agacccactt actagttttt tggttgtttc agccatggat
     4981 accttccaaa ttcttttatt tataagcatt ctactaaaca atagattatt catcattgaa
     5041 aatttctggc acatattttt cgtgcacttt taatcgcctc atgtaattag tattattacg
     5101 gatactgatt tataggcaaa taaatttaca gggatttaga tatgaaacta ggtttattcc
     5161 ttatgccctc ccaccctcca gaaaggtcgc cgtacgacgc ccaacaatgg gatctcaaat
     5221 gtttacgtat ggcggaccaa cacggccttg cagaggcttg gataggggaa cactttacgg
     5281 ctccttggga accggtgccc accttctacc ctttcatcac cctatagagc ttgctgcaag
     5341 ggttgcatat ttggatcatt tggcccaagg cagatatatg tttggaatag gttcgggcgg
     5401 tcttcagtca gaccatgaat tattcggcgt cgatatggaa aatggtgaac atagagaaag
     5461 aactagagag tcactcgata taatcctaaa ccaatggaaa cacgtagatg gaccctttac
     5521 ttataaaggt aaattctggg acataaatat acccgacccc aaagactatg cgtttgccga
     5581 cctacgtcat cacataacgc catttcaaaa accgcatccg cctatagggg tcgccggagg
     5641 cagccctgga tctgagactc ttaagatagt tggagaacga ggttatattc ccatgagtct
     5701 agggttaaat gctgtttatg tggctaatca ctgggacgta gtaactaagg gtgcagagtc
     5761 agccgggcga cagccaccat ccagaagtga atggcgaatt gtacgagata tttgggtagc
     5821 tgatagtgat gaagaggcta gggaaggagc tatgaatggg atgcttggaa gagcttggaa
     5881 agaatatctg cgatctttat tcgaacaagg agcctacccc ttcgtatcct ttatgaagca
     5941 tgacgaaaat atgtccgatg atgatgtaac tatcgaatat atgatggaaa acctctggat
     6001 agtcggatcc ccaaaaaccg tcacagaaaa actccgaaat ctctatgcga cagttggggg
     6061 ctttgggcat cttttatggt taacattcga ccattctgaa aacagtgaag catatgaaac
     6121 ttccatgagg cttatggcag agaaggtgat gcctaattta tcggacctta ccggagacca
     6181 gctgccgatg ggttaattat tcttgccttc cgccgaaggc cggcattact tcttctgcaa
     6241 aaattcgtag ctgagacagg tattcgtccc tcggcatacc ttctggccag tgaagaataa
     6301 tgttttcgag gccagggtat tttgcttcaa cttctttaag aaatttgatt tggtgcgaag
     6361 gtggaccaca tatccatgct ttctgctcaa ctccatcctc tatcttagga gtcctcgctg
     6421 gggcgcccgg agttccccaa acacgccctt cttcatctgc atatctaaca aatccaaatg
     6481 gtgcaaacca tttaaatcgc tcgtcatgga acggacgtaa cctctccatg gcctctttct
     6541 cgttttccgc tatttaaaat ccgattccca gagccaaatt actacccaga acattccctc
     6601 tgccattttc tgagcatgca tctctaaacc tatgaaacac ctcatctacc aatttttctc
     6661 ccgtcaaggc aactaggcct ttaaagccat ttctggctat aaaatcgata cttcctccac
     6721 tcacaatagg ttgccaggtt tccaccggta gattcacggg ctttggaacc aaagttatct
     6781 cctcaaggtc atatcctctg tacgggacgg gtgcaggaat cgaatagttc ttcccatgat
     6841 gactgaatga ttcgctgtta aaagctttta aaataacctc tatttgttct tcaaataatt
     6901 ccctatttaa atcattatct ataaccgggg aaccaaacgt ctctacttca cgagagtgat
     6961 agcctcgtcc caccccgaat atcatccg
//
LOCUS       DASCNS010000183        17298 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001453846, whole genome shotgun sequence.
ACCESSION   DASCNS010000183 DASCNS010000000
VERSION     DASCNS010000183.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 17298)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17298)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17298
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001453846"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="QF838_06545"
     CDS             complement(<1..654)
                     /locus_tag="QF838_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60374.1"
                     /translation="MTKMTGGEALAKSLYREGVRVIFGLPGVQLYHLLDGLAKEPGIR
                     FINTRHEQATTYMADGYSRAGGGIGTALVVPGPGLQNASAGIGTAYSASSPILVVSGQ
                     IERDHIGLERGMLHEIKGQIETIKPITKHQKLILDPVKIPEAVHEAFKQLQTGRPRPV
                     QIEIPPETLSDTADIELLEAGKYERPVGSKESVSRGIDLISNAKNPLIWAGGGVISGE
                     "
     gene            complement(672..1835)
                     /locus_tag="QF838_06550"
     CDS             complement(672..1835)
                     /locus_tag="QF838_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60375.1"
                     /translation="MTLPNRMKFGIFLAPFHWLGDNPTLALERDLETIQWLDHLGFDE
                     AWIGEHHSAGWETISSPEVFMGVAAERTKHIKLGTGVASLPYHHPLMLTNRMVLLDHL
                     TRGRVMLGVGPGALVSDAYALGIEPSLQRPRMDEAIGIIKRLLTETEPITYESDWFTL
                     REALIHLRPYTEPHFPMAVAAAQSPSGMVAAGKHGLGVLSVSVVRGGSIASNLGDFWK
                     IAEDTAEKYHQTVDRNDWRIVLHVHLADTKKEAMDQAREKSAAYQYDYFHQTMGTPFD
                     YDGPKDKIIDYMVDNGAWCVGTPDDLIEKIKELGDQSGGFGGFMIQATEWGTREQVRH
                     SYELIARYVMPHFQGSLNNLVTSQKWSEDNRDNLQALKTVSLNKATESYKNRT"
     gene            complement(1945..2757)
                     /locus_tag="QF838_06555"
     CDS             complement(1945..2757)
                     /locus_tag="QF838_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HJO60376.1"
                     /translation="MVTWPDKILCPIVISFDLDGISGTINRNPASKNLPTLMSMREYG
                     PSIATPRILDLLDTHKIKSSFFIPGFIAETHPELVLDIHQRGHEIGNHGYMHEPPATL
                     TSQEEEQVLLQGSEILKDITGQSPVGYRSPSWELSPDSLEILKRNHFLYDSSMMGNDV
                     PYLVHTKESPMIEIPIHWELDDHPFFNYIPFLNQTNVMASPQHVYSVWSAAFEGLYKY
                     KRTFVLTMHPWIIGRPGRLNMLDKLITLIKSFQGTSFMSCKDLAVLKEHEYM"
     gene            complement(2769..4466)
                     /locus_tag="QF838_06560"
     CDS             complement(2769..4466)
                     /locus_tag="QF838_06560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HJO60377.1"
                     /translation="MKLSRLFVRTLRNAPSDTELISHKLLLQAGMVHQVSSGVYSYLP
                     LAWKSLRKIEQIIREEMDNAGAQEVKLGLLQPREIWQKSGRDEIYGPDMIRLQDRRGR
                     HLALPPTNEELMTETVKSFIQSYRDFPFTLYHIQTKFRDEPRPRGGLIRVREFDMMDA
                     YSFDINTEGLNLSYDLMISAYVNIFKRCGIETVIVEADSGAIGGKDSKEFILLSDSGE
                     DTIVMCDSCSYAANGEKSEFTKLSNPDEPETGQKEVPTPGVKTINQLCKYLSVGPEKT
                     LKSVFYKSDQEFLIVVIRGDLEINEVKLKNNISAVDLRLATKQEVAQQGLISGSASPI
                     GVKDIKVIADDSITLGNNFLAGANKEGYHLSGINHPRDFQAEILTDIALAEDGFLCPN
                     CIGTLHTKRGIEIGHVFKLGTRYSESMDAKFPDQDGNPQPIVMGCYGIGVGRILAGAI
                     EQHADNKGIIFPKTIAPYEVILTSLNTDKIQVMETSENIYTEMSRTGIEVLWDDRQES
                     AGVKFNDADLLGMPLRVVVSSRNIENNVVEIKIRNEQQGYTIPIENCIEEVARLLGK"
     gene            complement(4488..5639)
                     /gene="ispG"
                     /locus_tag="QF838_06565"
                     /gene_synonym="gcpE"
     CDS             complement(4488..5639)
                     /gene="ispG"
                     /locus_tag="QF838_06565"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HJO60378.1"
                     /translation="MRRVTKPVFVGDVKVGGDAPISVQSMTKTDTRDVKSTLIEIKEL
                     ENAGCEIIRCAVPDMEAAKALKEIKKGARIPVVADIHFHHELAIESLKSGVDCLRLNP
                     GNIRDEEKVKEVVKLAKQREVPIRIGVNFGSLPPVGAIGKTRGLNRHDDGVNLLTKGS
                     TPSSDYTAVDHMVATGLWEIDLLENLDFDLIKISLKAFDIDTTVDAYRRMSKLIPYPL
                     HLGITEAGTAKSGSIRSAIGLGVLLYEGIGDTIRVSLSDNAVEEVITGLEILKALGLK
                     KEGAILVACPSCGRADVDVRGLANQVDQLLQDTRTHIKVAVMGCEVNGPGEAKDADIG
                     IAAGVGKAVIFRKGKKSKIVDESNMLTELMAEIKQVEKELSQPTKKSVS"
     gene            complement(5671..6855)
                     /locus_tag="QF838_06570"
     CDS             complement(5671..6855)
                     /locus_tag="QF838_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HJO60379.1"
                     /translation="MPSWLLVIPVLSILVFVHELGHFLTAKRFGIKVTEFGFGFPPRL
                     WGISIGETLYSINLIPLGGFVKMVGEEDPTEPRSFARQSILKRSIVLTAGSFMNLLLP
                     LFIFAIILSLPHDTLVGTVTVSGVAPNSPAEESGLQEGDAILEINSLRISNHMELVKE
                     ISTKQGTEVELLIRKDNSRLGLLGSPEFATSKYIRLTPRETSPSYRVVEIVSESTKEI
                     SLSDAKQYNPSLEIGDVLRQGSIGVLIGTANSKVVKDQLPLHEAIPTAFSKIGEVISF
                     SAQGIGSLVSSGQNPGLTGPIGIAQVTGEVAKVGIAPIFELTALISISLGIVNLLPIP
                     ALDGGRLMFVLIEGIRGGKKVSPEKEGIIHMAGFILLIGLVVVMSYFDIIRILSGNSF
                     FR"
     gene            complement(6861..8003)
                     /gene="dxr"
                     /locus_tag="QF838_06575"
     CDS             complement(6861..8003)
                     /gene="dxr"
                     /locus_tag="QF838_06575"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HJO60380.1"
                     /translation="MNRSKKNISILGSTGSVGAQTLEVARSYPEDFNVVCLGANKNTQ
                     ILKEQILEFQPQAISCDFHKELSANGEIAEEIVSLSEIATYEGVDTVVVATSGNVALI
                     PTFEAIKKGKNIAIANKETIIMAGEQLTSLAESNSVSLMPIDSEPSAIWQCLRGENNN
                     ISKLIITASGGPFRNTPVRSLPNVTPTNALQHPTWKMGQKISVDSATMMNKAFEVIEA
                     RWLFNVPWENIEVIVHPESIIHSMVEFSDGSTKAQLSYPDMIVPIKHALFFPDRAPKS
                     NTNPFDPNKTKSLTFESLDQDKYPCFEIALKIAKRGGTWPSALSGADEAAVEAFLDSR
                     IKFTEIHTLIEQAIQNHESITKPTTAEMLKASHWAYDRVSELTSGN"
     gene            complement(8000..8827)
                     /locus_tag="QF838_06580"
     CDS             complement(8000..8827)
                     /locus_tag="QF838_06580"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HJO60381.1"
                     /translation="MKHRLISSAIALPLVLGGILLGGIWFSLIIIFGAVVSAYELARI
                     TGNNLRKEPFISAILAGLIVTFVQTYDSYLDGLPSINIEASTTILPIVLLSFVFTSIL
                     ILLMWGSDRKLGSFTCVVSSVSYTAGLLSFAVLARNSEFGLGWIVFLSLIVWANDTGS
                     YIIGKTVGKTPFFPTISPNKTVEGAIGGYVCGLAVAIASGLLLPHFGFVDLGLYSLLI
                     LGFIFVVLAQTGDLFESSLKRRANLKDSGSIMPGHGGALDRLDSIVLAFPVLYYFAQ"
     gene            complement(8874..9569)
                     /gene="uppS"
                     /locus_tag="QF838_06585"
     CDS             complement(8874..9569)
                     /gene="uppS"
                     /locus_tag="QF838_06585"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HJO60382.1"
                     /translation="MGKSKGPRHVAIIMDGNGRWATSQGLDRSSGHEKGTLNIQRVLQ
                     AFSSHGVKYITLYAFSTENWNRPKTEVDNLMRLTVSSIAEQLLFLHSEGIKITHLGEK
                     YSLPSEVVEAIKKCEKMTFHNDKLILNLAFNYGGRSEIVKAVKKLVEDKVSPPEISES
                     QIETRLYTKGIPDPDMVIRTAGEMRLSNFLIWQTAYSEYYSTETLWPDFNESDVSKAI
                     DVYRRRTRKFGEL"
     gene            complement(9582..10145)
                     /gene="frr"
                     /locus_tag="QF838_06590"
     CDS             complement(9582..10145)
                     /gene="frr"
                     /locus_tag="QF838_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HJO60383.1"
                     /translation="MEDIEEIFKNTKERMNKSVDSLQRDLSSVRTGRASTNLVENLDV
                     EYYGSSTPLNQLSSISVPEARTIVIQPWDKESLQEIEKSILKSDLGLTPNSDGVIIRI
                     NVPELTEERRREMVKLVGNLVEEGHVAIRNIRRDSLEKIRTLDKDKEISQDDGRRAQQ
                     ELQNVTDSFISEMDSIKSQKEKEVMEV"
     gene            complement(10154..10879)
                     /gene="pyrH"
                     /locus_tag="QF838_06595"
     CDS             complement(10154..10879)
                     /gene="pyrH"
                     /locus_tag="QF838_06595"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HJO60384.1"
                     /translation="MPDKPFKRILLKLSGESLKGNRSHGIDPDSLDYMAEEIKSVTSK
                     GIELGIVVGGGNIWRGSEAEIEGMDRASADYAGMLATVISALALQDALERKHKLNTRT
                     LSAINIQQVAEPYIRRRAIRHLEKGRIVLFAAGTGNPFVTTDTGSALRALEIDADVLL
                     MAKNDVDGVYDKDPKLDKSAVRYDSISYLDAIEKRLKVMDSTALSLCMEYSIPIVVFD
                     LFEKGNLNRIVSGEKVGTLVTGE"
     gene            complement(10887..11390)
                     /gene="tsf"
                     /locus_tag="QF838_06600"
     CDS             complement(10887..11390)
                     /gene="tsf"
                     /locus_tag="QF838_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HJO60385.1"
                     /translation="MPATTTQLIKELRARTSAGVMDCKKALEESKGDIDKAESFLKEK
                     GVASAAKKADRDTDQGLIDTYIHSGGRIGAMIEINCETDFVARTNDFAGLAHDIAMQV
                     AAMSPEFLTMKDAPENDTTTEDKCLLSQPFIKDPSKTIQDLINETVGKLGENIRVRRF
                     QRFSLGE"
     gene            complement(11430..12467)
                     /gene="rpsB"
                     /locus_tag="QF838_06605"
     CDS             complement(11430..12467)
                     /gene="rpsB"
                     /locus_tag="QF838_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HJO60386.1"
                     /translation="MVEEAVEVETIQTQDPEAITMKSLLEAGVHFGHQKRRWNPKMKR
                     YIFTHRNGIHIIDLQKTLNLVEEAAAFLSEVAASGKKILMVGTKKQAQDTIVTEAERS
                     NSLHISNRWLGGTLTNFKTIQKRIDYLVDLETRRDQGDFENLTKKESLKIDEKITKLN
                     KNLSGIKEMTEMPGAMFIIDVGREEIAVAEANRVGIPIVALVDSDCDPDLIDHPIPGN
                     DDAIRSVRLITGRMSDAIIDGYHQRNAAQSDDPTDPNQASNPRMVTYSTVTIEDSISE
                     ETESEENEDAEETIIEPEQNVESDESESEPSEIDKEVISPEIEESTPNPENSVSSDST
                     EKESEPDEKVN"
     gene            complement(12616..13245)
                     /locus_tag="QF838_06610"
     CDS             complement(12616..13245)
                     /locus_tag="QF838_06610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO60387.1"
                     /translation="MDKPKGNNQSIRKMKNEDISQVSLIESDAFPQLFPPTSFRKELQ
                     REISTILVAISNSNTGEKKESSPHSKPIKSPVSYRSGNTGWKPGMDFLTGYIFLWDTT
                     FEESHIMSIGTRRSHRRKGIGELLLYSALINSIKKGARSLTLEVRVSNIPAISLYQKY
                     GMTTQGLRKSYYTDNREDAKIMTVADIQSLDYRHLLNSKWKKLEKRLKK"
     gene            13454..15223
                     /locus_tag="QF838_06615"
     CDS             13454..15223
                     /locus_tag="QF838_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012869.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO60388.1"
                     /translation="MQHILRIIRIALRYKYRIAFAYLSTIGAIVAYIFLPKLFGDAID
                     SVAEPILAGQKVDTGILYPCILIILGLSVIRGGMSYFQTYLAETLSQYVSYDLRNAFY
                     DHVQHQSFAFHDRHHTGNLMSRAITDVENIRMFINMGLVRAPYFIALFIIVAAILISM
                     NWKLGLLSASFMPIVAIYTSRVRLRMRALWLLVQQKMAELSIILQENFSGQRVVKAFA
                     SEDHEEDKFEIKNAEVSDIYVSAEKLRASSMSFMMFTFMVAMALIIWYGGRQVMNGTM
                     TPGELAAFVFYLQILALPVRMAGWVVNAYARAASAGERMFEILDAPSPVEETSNPITL
                     EKSKGHVRFENVSFAYDGGKPVLRNINLEAKPGEIIALVGPPGSGKSTIANLLPRFYD
                     VSAGVIKIDGIDVKEFTLRSLRENIGMVQQDVFLFTTSLKENIKYGRENASDEQMENA
                     SSIAQMADFIEGLDEGYDTYVGERGSTLSGGQRQRMSIARAILLDPPILILDDSTSSV
                     DANTEDLIKAAMDNSMEGRTTFIIANRLSTVHSADKIVVLKDGRIIEEGSHDQLLRQG
                     GLYNEIYELQLRPQDELSSEVSR"
     gene            15220..17124
                     /locus_tag="QF838_06620"
     CDS             15220..17124
                     /locus_tag="QF838_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO60389.1"
                     /translation="MNLGLGGMSASRGQADEEFFGSVYDQKVILRLLPYIKPFTPLVI
                     VSFVSMLIYTFTLVAVPWLIQWGIDNPIKDGLQTATDYKETVSLAGEETNLAILKELL
                     SPHIKILNILFVVFLINAFTNWGTNYLQQYAMQKVGQGVLFRLRRALFGHVQKQSLSY
                     FDTTEVGRLMSRVQGDVGQLQEFAALVVMSLGELLSLVGIVIALLVMDLELGLITMGV
                     LPVLMIITVIWQKYAKEAFSEIRRRIAIVNAAFNENISGVRVVQSLNRQERNLEIFDE
                     KNTAHRWSNVIAGRYAAGLLPAVDILTAVAIGLALYFGSKLVGLEEGQLLPGVLVAFV
                     MYVQRFFDPIRNLTQQYTQLQRSMASGARIFDLMDREPELVDIDDAIEMPEIKGKVEF
                     KNVSFRYVEGEDVLKGINLKVNAGETVAVVGPTGAGKTSIVSILSRFYPVKREEGEIL
                     IDGIDIRDVKRASVVGQMSMVLQEPYLFSGTVMENIKYNHVDATDEKAISSAKVVGAH
                     EFIMQLEDGYDTFLSERGANISLGQRQLISFARAIVDDPKILILDEATASIDSHTELV
                     IQTALRKLLEGRTAIVIAHRLSTIRGADKIVVLEAGEVVEVGNHQELMDKDGLYAHLY
                     NMNFQSIEDL"
BASE COUNT         4484 a         3666 c         3537 g         5611 t
ORIGIN      
        1 ttctccagat atcacacctc caccagccca tataagagga ttttttgcat tggaaataag
       61 atcgattccc cgacttacgc tttctttact gccaacaggt ctctcatatt ttccagcttc
      121 caataactca atgtcagctg tgtcggaaag ggtttctggg ggtatttcta tttgaaccgg
      181 ccttggcctg cctgtttgaa gttgcttaaa ggcctcatgt acggcctctg gtatcttaac
      241 gggatccagt attagttttt gatgtttggt aatgggtttt attgtttcaa tttgaccttt
      301 tatttcatgg agcattcctc tttcaaggcc aatgtggtct ctctcaattt gacccgagac
      361 aactaagata ggagatgatg ctgaatatgc tgtacctatt cctgcactgg cgttttgaag
      421 gcccggtcct ggcacaacca atgctgtgcc tattcctcct cctgcccgtg aatatccatc
      481 agccatgtag gtagtagctt gctcatgccg ggtattaata aaccgtattc cgggttcttt
      541 tgccaatccg tcaaggagat ggtaaagctg tacccccggc aacccaaaaa ttactcttac
      601 cccttctcgg taaagtgatt tcgcgagagc ttcgcctcca gtcatttttg tcatgaatgt
      661 ttctcctagt tctaggttct atttttgtag ctttctgttg ccttgttgag agatacagtt
      721 ttcaaggctt gcaagttatc tctattatct tcagaccatt tttgagatgt caccaaattg
      781 ttaagtgagc cttggaaatg gggcattacg tatcgggcaa tcaattcgta actgtgccgt
      841 acttgttctc tagttcccca ttctgtggct tgaatcataa aaccgccaaa ccctccgctt
      901 tgatcaccca gttctttgat tttttctatg agatcatctg gtgttccaac acaccaagcc
      961 ccgttatcaa ccatgtagtc tattattttg tccttgggtc cgtcgtaatc aaaaggtgtt
     1021 cccatagttt gatggaagta gtcgtattgg tatgcggccg atttttctct tgcttggtcc
     1081 atagcttctt ttttggtgtc agctagatga acgtgtaaga cgattctcca gtcatttctg
     1141 tcgactgttt gatgatattt ttccgctgta tcttctgcga tcttccagaa gtctcccaaa
     1201 ttagatgcta tgctcccacc tcttactacg ctaacagaaa gcactcccag gccatgctta
     1261 ccggctgcaa ccatccctga aggtgattgt gctgctgcaa cggccatcgg aaaatgtggt
     1321 tctgtgtagg gtctcaaatg tattaaagcc tctcgtaaag tgaaccagtc agattcatag
     1381 gttataggct ctgtttctgt aagaagcctt tttattatgc ctattgcttc gtccatgcgg
     1441 ggtctttgta aagatggttc aataccaagt gcataggcat ccgaaaccaa agcacctggc
     1501 cctacaccca acatcactct tcctcgagtc aaatgatcta ataaaaccat acgatttgta
     1561 agcataagag gatggtgata tggcaaactg gcgacccctg tgcctagttt tatgtgtttg
     1621 gttctttctg ctgcgactcc cataaatacc tcaggagaag atatagtctc ccatcccgcg
     1681 ctgtggtgct cacctatcca agcctcatcg aaacctagat gatccaacca ctgtatcgtt
     1741 tccagatccc tttctagggc gagagttggg ttgtcgccca gccagtggaa aggtgctagg
     1801 aaaattccaa atttcatgcg gttgggtaag gtcatttggt ttattttgct ccttgttata
     1861 atggcaaacc atgtatacgg cagattacag tggggattaa accgtgaaat gttatcatca
     1921 ggcctatccc ttcttcaaac ccttttacat gtattcgtgt tcttttagga cagcaaggtc
     1981 tttacatgac atgaaagagg ttccttgaaa actttttatc agggttatca atttatccag
     2041 catgttgagc ctgcctggtc ttcctataat ccatgggtgc atagttaaga caaaggttct
     2101 tttatattta tataaacctt caaatgccgc agaccaaacc gaatatacat gttgagggct
     2161 cgccatgaca ttagtttgat tcaggaaagg aatgtaattg aaaaacgggt gatcatccaa
     2221 ttcccaatgt atcgggattt ctatcatagg tgattctttg gtatgtacaa gatatggaac
     2281 gtcatttccc atcatacttg agtcatatag aaaatggttc ctttttaata tttccaatga
     2341 atcaggactc aattcccaac taggagatct ataccctact ggagactgac ctgttatatc
     2401 ttttagtatt tccgatcctt gcagtagtac ttgttcctcc tcttgtgagg tcaatgtggc
     2461 aggaggctca tgcatatagc cgtggtttcc tatctcatgg cctctttgat gaatgtccaa
     2521 taccaattct ggatgtgttt cggctatgaa gcctggtatg aaaaaagaag attttatttt
     2581 gtgtgtatcc aataggtcta gaattctcgg ggtcgcaatc gaaggaccat actctctcat
     2641 agacattaga gtaggtaaat tcttggatgc tgggtttctg ttaatggttc cactgatgcc
     2701 atctagatca aaacttatca ctatcgggca aagtattttg tcaggccaag ttaccatttt
     2761 agatccgttt actttcccaa tagtctggcc acttcttcaa tgcaattttc aattgggata
     2821 gtgtatccct gttgttcatt ccgtattttg atttcgacta cattgttttc tatgtttcgg
     2881 ctacttacaa ccactctcaa tggcatacct aataggtctg catcattgaa tttgactccc
     2941 gcagattctt gcctatcgtc ccaaagcacc tcaatgcctg ttcgtgacat ttctgtgtat
     3001 atgttctctg atgtctccat gacctggatc ttatcagtgt tcagagatgt aagtatcacc
     3061 tcgtatggag caatggtctt cgggaatatt atccctttat tatcagcatg ttgttctatt
     3121 gccccagcga gaatccgtcc gaccccaatt ccatagcatc ccatgactat aggttgtgga
     3181 tttccgtctt ggtctggaaa cttggcatcc attgactcgg aataacgcgt gcctaattta
     3241 aatacgtgcc caatttctat ccctcgtttt gtatgcaagg ttccgatgca attcggacat
     3301 aaaaacccat cttctgccaa ggcaatatcg gttagtattt cagcttgaaa atctctcgga
     3361 tgatttattc ctgaaagatg gtatccctct ttatttgcac ctgccaaaaa attgttgccc
     3421 aaagtgatag agtcatctgc aatcacctta atatctttca ctcctattgg agaggcagaa
     3481 cccgatatta atccctgttg agcaacttct tgttttgtgg ctagtcttag atcaactgca
     3541 cttatgttgt tctttaactt cacttcgttg atttccaaat caccacgaat aaccacgata
     3601 aggaattctt ggtctgattt atagaagact gacttgaggg ttttttccgg gcccactgag
     3661 agatatttgc agagttggtt tatggttttt accccgggag tagggacttc tttttgcccc
     3721 gtttcaggtt cgtcgggatt cgatagcttg gtgaattctg atttttctcc attggccgca
     3781 taggagcaac tgtcacacat tactattgtg tcctctcctg aatccgatag aagaataaat
     3841 tctttagaat ctttgccccc gattgcaccg ctgtctgcct ctactatcac agtttcaatt
     3901 ccgcagcgct tgaaaatgtt gacatatgca ctgatcatta agtcataact caggtttaac
     3961 ccttccgtat taatgtcaaa gctgtaggca tccatcatgt caaattctct aactcttatc
     4021 agaccacctc tgggcctagg ttcatcccta aattttgttt ggatatggta caaagtgaat
     4081 ggaaaatccc tgtaactttg gatgaaactt tttactgtct cggtcatcaa ttcttcattg
     4141 gtaggaggca aggctaaatg ccgtcctcta cggtcctgga gtctaatcat gtccggcccg
     4201 taaatttcgt ctcttccgga cttttgccaa atttccctcg gctgcaataa ccctagtttt
     4261 acctcttgcg ctcctgcatt atccatttct tctctaatga tctgttcaat tttgcgcaac
     4321 gatttccatg cgagtggtaa gtagctgtat acgccagaag aaacttggtg caccattccg
     4381 gcttgcagca ataacttatg gcttattaat tcagtgtcac taggggcatt tctcaaggtt
     4441 cttacgaata atcgggataa tttcatataa taaatgcctt ctaggaatca actaacagat
     4501 ttttttgttg gctgtgataa ttctttttcg acttgtttaa tttctgccat cagttcagta
     4561 agcatgttgg attcatccac gatttttgac ttttttcctt tacggaaaat cactgcttta
     4621 cccaccccgg cggcaattcc aatatcagca tctttggcct ctcccgggcc attgacctca
     4681 catcccataa cagcgacctt gatatgtgtt cttgtatcct gtagtagttg gtcaacttgg
     4741 ttggccaagc cccttacatc tacatcagct cttccgcaac tcggacaggc tacgagaata
     4801 gctccttctt tttttaggcc taatgctttt aatatttcaa gcccagtaat cacctcttcg
     4861 actgcattat cactaaggct tacgcgtatt gtgtctccaa taccttcata aagtaatacg
     4921 cccaatccta ttgcacttct tatactgcct gattttgctg taccagcttc tgttataccc
     4981 agatgaagag ggtatgggat caattttgac attcttctgt aggcatctac tgttgtatct
     5041 atatcaaagg cttttaggga tattttaatt agatcaaaat ccaaattttc aagtaggtca
     5101 atttcccaca ggccagtagc aaccatatga tctacggcag tatagtctga gctaggtgta
     5161 cttcccttgg ttagtaggtt taccccgtca tcgtgcctgt tcaagccacg tgttttccct
     5221 atagctccta ctggaggaag acttccgaaa tttacaccta tcctgattgg cacttctcgc
     5281 tgtttggcca atttgaccac ctcttttacc ttttcttcgt cccttatgtt tcctgggtta
     5341 agccttaggc aatcaacgcc gctttttaac gattctatag ccaattcatg atggaaatgt
     5401 atgtctgcaa cgacaggtat acgtgcacct ttttttattt cttttaatgc ttttgcagcc
     5461 tccatgtcag gcacggcaca gcgaattatc tcgcaacccg cgttttccag ttcctttatt
     5521 tcaataagtg tagatttcac atctctggtg tcagtttttg tcatggattg gaccgatatg
     5581 ggcgcgtccc caccaacttt tacgtcgcct acaaaaacag gttttgttac tctacgcatt
     5641 taaaatccct ggtgatttat taattaataa ctacctgaaa aaactatttc cacttaatat
     5701 tcggattatg tcaaaataac tcatcaccac caccagtccg attagtagaa tgaatcccgc
     5761 catatggatg attccttcct tttccggaga aaccttcttg ccacctcgaa ttccttcaat
     5821 caatacgaac attagtctac ctccatccag agcagggatg ggtaatagat taacgattcc
     5881 caagctgatg ctgattaagg cggtaagttc aaaaataggg gcgattccca ccttagcgac
     5941 ttctccggta acctgagcaa tcccgattgg accggtcaat ccagggtttt ggccactact
     6001 tactaaacta ccgattcctt gtgctgaaaa tgagataact tcacctatct tactaaatgc
     6061 tgttggtata gcttcatgca aaggaagttg atctttaact actttactat tagcggtccc
     6121 gattaatacc ccaatagaac cttgacgtaa aacgtctccg atttctaggg atggattgta
     6181 ttgttttgca tcagatagtg atatttcttt tgtggattcc gatactattt ctactaccct
     6241 gtaggaagga gaggtttccc ggggtgttag gcgaatatac ttagaagtcg caaattcggg
     6301 agatccaagt aaccctaaac gtgaattgtc ttttcttatt aacagctcga cttcagtgcc
     6361 ctgcttggtt gatatttctt taacaagctc catatggtta gagattcgta aggagtttat
     6421 ttctagtatt gcgtcgcctt cttgaagccc agattcttca gcaggtgaat ttggtgctac
     6481 cccactgaca gtaacagtac caactaatgt gtcgtggggt agtgaaagga taattgcaaa
     6541 tataaataaa ggtaaaagga gattcataaa cgaaccagca gtcaagacaa ttgatcgttt
     6601 gaggattgat tgtcttgcaa aacttctggg ttctgtaggg tcttcttcac caaccatttt
     6661 gacaaatccg ccaagaggaa ttagattgat ggaatataag gtttctccaa tagatattcc
     6721 ccacaaccgg ggcgggaaac caaatccaaa ctccgtcact ttgattccaa aacgcttagc
     6781 tgttaaaaaa tgaccaagtt catgtacaaa tactagaatc gacaataccg gaataactag
     6841 taaccaactt ggcatggata tcaattacct gaggtcagtt cggacactcg gtcataggcc
     6901 caatgggatg ctttcaacat ctcagcggta gtaggttttg ttattgattc gtggttctgt
     6961 atcgcctgtt cgatgagagt gtgtatttca gtgaatttga ttctgctatc aaggaatgct
     7021 tccacagcag cttcatcggc tccgctaaga gctgaaggcc aggtccctcc tcgcttggca
     7081 attttcaaag caatttcaaa acacggatat ttgtcttgat caagactttc aaaagtgagc
     7141 gatttagttt tatttggatc gaaagggttt gtatttgatt ttggtgcccg atccggaaaa
     7201 aacaaagcat gctttatcgg tacaatcata tcgggataac ttagttgagc ttttgttgaa
     7261 ccgtctgaaa attcaaccat tgaatgtatt atgctctcag gatgtacaat aacttctata
     7321 ttttcccacg gtacattgaa taaccaccta gcctcaatca cctcgaacgc tttattcatc
     7381 attgtggcag agtcaacact aatcttctga cccattttcc aggtcggatg ctgcaatgca
     7441 ttagtgggag tgacgtttgg taaagatctc acaggggtat ttctaaatgg tcccccggat
     7501 gcggttatta tcaatttgga aatattgtta ttttctcccc gtaagcactg ccagattgca
     7561 ctcggttcgc tgtcaattgg catcaagctg acggagtttg actcggctag agaagttaat
     7621 tgctctcctg ccattatgat cgtttcttta tttgcgattg ctatgttctt ccctttttta
     7681 attgcttcaa aagtgggaat caaagccaca tttccggatg ttgctactac gacagtatcg
     7741 actccttcat aggtggctat ttcagaaaga gacactatct cttcggctat ttcaccattg
     7801 gcactcaatt ctttatggaa atcgcaggat attgcctgag gttgaaattc aagaatttgt
     7861 tcttttaata tttgtgtatt tttattagca ccgaggcata ctacattgaa atcttcagga
     7921 taacttcgcg ctacttccag tgtttgagca cccacggagc ctgtagaacc gaggatggat
     7981 atgtttttct tactcctatt cattgtgcaa aataatatag cactggaaaa gccaatacaa
     8041 tcgagtctaa cctgtccaaa gcccctccat gccctggcat tattgaacct gaatctttta
     8101 ggtttgctct ccttttaagg gaagactcaa ataaatctcc tgtttgggcc aatacaacaa
     8161 aaatgaaacc tagtattaat agtgaatata accctaaatc gacaaatccg aaatgaggga
     8221 gtaataggcc gctggcgatg gcgactgcca aaccacaaac gtacccccca atagctcctt
     8281 ctacagtctt gttgggactt atggtaggga agaatggggt tttaccgacc gtctttccta
     8341 ttatgtatga ccctgtatca ttggcccaaa ctatcaatga taaaaatacg atccaaccga
     8401 ggccaaactc actatttcga gcaagaacag cgaacgaaag caatcctgca gtatatgaca
     8461 ctgatgagac aacacatgta aaactaccta gttttctatc gctaccccac atcaaaagta
     8521 ttaggatgga tgtaaatacg aacgatagaa gaactattgg taatatggtg gtgcttgctt
     8581 ctatgttaat actgggtaga ccgtctagat aactgtcata cgtttgaaca aacgtaacta
     8641 tcaacccagc tagaatagct gatataaagg gctcttttcg gagattgtta ccggtgattc
     8701 ttgccagttc ataggctgag acaactgcac caaatattat tatgagggag aaccatatcc
     8761 ctcccaacaa tatcccccca agtactaagg gaagtgctat agcagaactg attaatcggt
     8821 gtttcacttt atattttcca gcaacctaat atacccatag cccgtgtgaa gcttcataac
     8881 tctccaaatt ttcgggttct acgtcgatat acatctatag ctttggatac atcggactca
     8941 ttaaaatcag gccaaagagt ttcagttgaa tagtattctg aatatgctgt ttgccatatt
     9001 aaaaaattac ttaatctcat ttctcctgct gtgcgtataa ccatatcggg gtctggtata
     9061 ccctttgtgt aaagtcttgt ctcgatttgg gattcactaa tttctggtgg gcttacctta
     9121 tcttcgacta gtttttttac tgctttcact atttctgacc tgccaccgta attgaaggca
     9181 aggttgagaa ttaatttatc gttgtggaag gtcatttttt cgcatttttt tattgcttct
     9241 acaacttcgg aaggcaagga atatttttct ccaagatggg taatttttat accttcagaa
     9301 tgcagaaata gtagctgttc agcaatggaa gatacggtta gcctcataag gttatctact
     9361 tcggtctttg ggcggttcca attctctgta gagaaggcgt aaagggtaat atacttcacg
     9421 ccgtgggatg aaaatgcttg gagcaccctt tgaatattta gtgttccttt ctcgtgacca
     9481 gagcttctat ctagtccttg ggaggttgcc catcttccat tgccgtccat aattatggct
     9541 acatggcgtg gacctttact tttacccata gtacaactgg gtcacacctc cataacctct
     9601 ttttcttttt gagacttgat tgaatccatt tcagatatga aggagtcggt gacattttga
     9661 agctcctgct gagctcttct accgtcgtct tgagagatct ccttgtcttt atctaaagtt
     9721 cttattttct ccaaactatc tctacgtata tttcttattg ctacgtggcc ttcttcaacc
     9781 aagtttccaa ccaacttaac catctcacgt cttcgctctt cagttaattc aggtacgttt
     9841 attcttatga ttactccatc agaattagga gtcaggccaa gatctgattt caatatactt
     9901 ttctctattt cttgtaggga ttccttgtcc caaggttgga taactatggt ccgggcttca
     9961 ggtactgata tcgaggataa ttggtttaaa ggtgtggagc taccataata ttcaacatca
    10021 aggttttcaa ctagattggt tgaagctcgt ccggttctca ccgaggatag atctctttgc
    10081 aaagaatcaa cacttttgtt cattctttct ttggtgtttt taaatatttc ttcaatgtct
    10141 tccatgatct ttattactcc cctgtgacca aagtaccgac tttttcacct gagacgatac
    10201 ggtttagatt ccctttctca aaaaggtcaa aaacaacgat tgggatactg tactccatac
    10261 ataacgacag agccgtacta tccattactt taaggcgttt ttctattgcg tctaaatatg
    10321 aaatactgtc atagcgaact gcggatttat ctaatttggg atccttatcg taaaccccgt
    10381 ctacatcatt tttcgccatt agtaatacat ctgcgtctat ttccaaagct ctcaacgcag
    10441 atcctgtgtc cgttgtcacg aatgggttgc cagtaccagc tgcaaacaat acgatacgac
    10501 ctttttcaag gtggcgtatt gcccttctcc ggatgtatgg ttccgccact tgttggatat
    10561 taattgcact aagagttctt gtgttgagtt tgtgctttct ctctagtgca tcctgtagtg
    10621 ccaaggcgct tattacagtt gcaagcattc ctgcataatc ggcagaggct ctgtccatac
    10681 cttcaatctc tgcttctgaa cctctccaga tatttccgcc tcctacaaca atccctagct
    10741 caataccttt tgaagtgact gactttatct cttcagccat ataatccagg gagtcaggat
    10801 ctattccgtg acttcggttt ccttttagcg attctccact tagctttagc agaatccgtt
    10861 tgaatggttt atctggcata tttgcctcat tcacccaagg aaaatctttg gaatctccgt
    10921 acccttatgt tttccccaag ctttccaaca gtttcgttga tcaggtcttg gatagttttt
    10981 gaggggtctt tgataaatgg ctgtgataac aaacatttat cttcggttgt tgtgtcattt
    11041 tccggggcat ccttcatcgt aaggaattcc ggtgacatag ctgcgacttg cattgcgata
    11101 tcgtgggcta gtcctgcaaa atcatttgtt cgggccacaa aatcagtttc acagttgatc
    11161 tctatcattg ctcctatcct gccgccgctg tgtatgtagg tatctattaa accttgatct
    11221 gtgtccctat ctgctttttt tgcagctgat gcaactcctt tttccttcaa gaaggattca
    11281 gctttgtcta tgtcgccttt actttcttcc aaagcctttt tgcaatccat aacgcctgcg
    11341 cttgttcggg cgcgcaactc tttgatcagt tgtgtggtag tagcaggcac attctctccc
    11401 ttgaaattga gttagatggg ttttttcagt cagttgacct tttcatcagg ctcagattct
    11461 ttttcagtag agtctgaact taccgaattt tcaggattag gcgtggattc ctcaatttca
    11521 ggagaaatta cttccttgtc gatttctgaa ggttcggatt ccgattcatc actttcaacg
    11581 ttttgctctg gttcgatgat tgtttcttca gcatcttcat tttcctcaga ttcagtttcc
    11641 tcagaaattg aatcttctat ggtaacagta gagtaagtaa ccattcttgg attcgatgct
    11701 tgatttgggt ctgtgggatc atcagattgc gcagcgtttc gttggtgata gccatctata
    11761 atggcatccg acattcttcc cgtgattaat ctgacagacc ttatcgcatc atcgtttcct
    11821 gggattgggt ggtcaataag atctggatca cagtcagagt ccaccaatgc cactattggt
    11881 ataccgactc gattagcttc agctacagct atttcttctc ttcccacatc gataatgaac
    11941 attgcccccg gcatttcggt catttccttt attccggaga ggtttttgtt taatttggtt
    12001 atcttctcat caattttcaa agactctttt ttcgttaggt tttcaaagtc cccttgatcg
    12061 cgccgagtct ccaaatctac caaatagtca attctcttct gaatagtttt gaagttggtt
    12121 agggtccctc ctagccacct gttggatatg tgcaaggaat tacttctttc agcttcagtc
    12181 acaatcgtat cttgggcttg cttctttgta ccgaccatca gaatcttttt tccacttgcg
    12241 gctacctcac tcagaaatgc tgcagcttct tctaccaaat tcaaagtttt ctgtaggtcg
    12301 attatgtgaa tgccatttct gtgagtgaag atataccttt tcatcttcgg gttccatcgc
    12361 cttttctggt ggccaaaatg aactccggcc tccaaaagtg atttcatcgt tattgcttca
    12421 ggatcctggg tttgaattgt ttctacctca acagcctctt cgaccatgta ttttcctttc
    12481 actatgatag gttggttggc tattttaaag cccttaaacc gtgattatct aactgtgctt
    12541 tgcaactgaa aaaacgccgg aaaagtataa catgacacta cttcgacagt aaaagcaaat
    12601 tttagaaaat aagaattatt ttttgaggcg cttttctaac tttttccatt tggagttcag
    12661 taaatgtcgg taatctaggg attggatatc agctaccgtc ataattttgg catcctcacg
    12721 gttgtcagtg taataggatt ttcttaatcc ttgtgtagtc attccatatt tttggtatag
    12781 agatatagct ggtatatttg aaaccctcac ttcgagggtc aaagaccttg ctcctttttt
    12841 aatcgagttt attaaagctg aatatagaag caattctcca atccctttcc ttctatgaga
    12901 tcttctagtg cctatggaca tgatatgaga ttcttcaaaa gttgtatccc agagaaaaat
    12961 atatccggtc aggaagtcca tcccaggttt ccatcctgta tttccagacc gatagcttac
    13021 agggcttttg attggcttgg aatggggtga ggattctttt ttctctcccg tatttgagtt
    13081 agatattgca actaggatag tcgaaatttc cctttggagc tcctttctaa atgaagtggg
    13141 aggaaacaat tgaggaaagg catccgattc aataagtgaa acttgagaga tatcctcatt
    13201 cttcattttt cgtatggatt ggttattacc tttgggttta tccatttcga aagggtttac
    13261 ctcgtgcagt ctttttgttt ttaattatgc cttttaaaga catgtattag attacatgaa
    13321 caaattgtca agaaattgtt ttcaaaacac ttgatattta gatgacaact caatgtagaa
    13381 taaggagtcc gtgtggattc cgagcttttt tgcttcgtta gccacaactc ttgggccaag
    13441 tttcagggaa tctatgcaac atatccttag aataataaga attgctctga ggtacaaata
    13501 tagaatagct tttgcttact tatctacgat aggggctata gtagcttaca ttttcctgcc
    13561 caaactattc ggagacgcaa tcgactctgt tgcagagcct atcttagccg gacagaaagt
    13621 ggacacagga atcctttacc catgcatatt aattattttg gggttgagcg tgattcgtgg
    13681 aggtatgtcc tatttccaga cttatttggc agagacattg tcgcaatatg tttcttacga
    13741 cctaagaaat gctttctatg atcatgtgca gcatcagagt tttgcttttc atgataggca
    13801 ccacaccggt aatctgatgt ctcgagccat cacagatgtg gaaaacatac ggatgttcat
    13861 aaatatggga cttgtcaggg ccccatattt catagctttg ttcataatag tcgctgctat
    13921 tttgatttcc atgaactgga aattaggact cttaagcgct agctttatgc ctatagttgc
    13981 tatttatacc agccgagttc gacttaggat gagggcatta tggcttttag tacaacaaaa
    14041 aatggcggaa cttagtatta ttcttcagga gaatttctca ggccaacgag tcgttaaggc
    14101 ttttgcttca gaggaccatg aagaggataa atttgaaata aagaatgccg aagtttcaga
    14161 tatttatgtg tctgcagaga agttgcgggc ttctagcatg tctttcatga tgtttacttt
    14221 catggtggcc atggctttga taatttggta cggggggcga caagttatga atgggactat
    14281 gacgcctggt gaattagcag cctttgtttt ttatctccag atactggcct tgccggttag
    14341 gatggcaggg tgggtagtaa acgcgtatgc cagagctgct tctgcaggag aaaggatgtt
    14401 cgagatactt gatgcaccat ctccggtcga agaaacatca aacccaatta cacttgaaaa
    14461 atctaaaggg cacgttagat ttgaaaatgt gtcttttgcc tacgatggcg gtaaacctgt
    14521 tctcaggaac ataaatttgg aggcaaaacc tggtgagata atcgcattgg ttggaccccc
    14581 agggagcggt aagtcgacta ttgccaacct tcttccaaga ttttacgacg tttctgcagg
    14641 agtaataaaa atcgatggga tagatgtgaa agagttcact ctgcgttcct taagggagaa
    14701 cattggaatg gtccagcagg acgttttcct cttcactacc tcgttaaagg aaaacattaa
    14761 atatggtcgt gagaatgcta gcgatgagca gatggaaaac gcctcttcaa tcgctcaaat
    14821 ggcagatttt attgaaggtc ttgacgaggg ttatgatacc tatgttgggg aaagggggtc
    14881 cactttatcc ggaggacaac gccagcgaat gtcgatagct cgagcgattt tgctagaccc
    14941 tcctatatta atattggatg attcaacttc cagcgtggat gcaaatactg aagatctaat
    15001 aaaggccgca atggataatt caatggaagg aagaacaaca ttcattatcg ccaacagact
    15061 tagtaccgta cacagtgctg acaagatagt cgtcttgaag gatgggagga tcattgaaga
    15121 aggttctcat gaccagttgc taaggcaagg tggcctttac aatgaaatat atgaattgca
    15181 attgcgaccc caggatgaat tgagctctga ggtgagtaga tgaatctcgg cttaggcggt
    15241 atgtctgcct cgagaggcca ggcagacgag gaattttttg ggtccgtata tgatcaaaag
    15301 gttatcctga gactacttcc ttatatcaaa ccctttacac ccctggtaat tgtttccttc
    15361 gtttcgatgt tgatttatac ctttactttg gtggcggtac cttggctcat acaatggggt
    15421 atcgataatc ctatcaagga tgggctgcaa actgcgactg actacaaaga aactgtaagt
    15481 cttgcaggag aggagactaa tctagcaata ctcaaagaac ttctttcacc tcatattaag
    15541 atactcaaca ttcttttcgt tgttttttta attaatgcgt ttaccaactg gggtacgaat
    15601 taccttcaac agtatgcaat gcagaaagtg ggtcaaggtg tcctgtttag gttgagaaga
    15661 gctttgtttg gtcatgtgca aaagcaatcc ctgtcctatt ttgataccac agaagtgggt
    15721 aggcttatgt cacgtgtaca aggagatgtt ggtcaactgc aggagtttgc agctttggta
    15781 gttatgagcc tcggtgagct gttgagtctt gtaggtattg tcattgccct cttggtgatg
    15841 gacctagaat tgggtcttat tacgatgggg gtactgcctg ttttgatgat tattacggtc
    15901 atttggcaaa aatatgctaa agaggccttt agtgaaatca gacggcgaat cgcgatagta
    15961 aacgccgcgt tcaacgaaaa tatttcaggg gtaagggtag tccaaagtct taatagacag
    16021 gaaaggaatc tagaaatatt tgatgaaaaa aatacagctc ataggtggtc caacgtaatc
    16081 gcaggtagat atgccgcagg ccttctgccg gcggttgata ttcttactgc tgttgcaatt
    16141 gggcttgcct tgtattttgg ttccaaattg gttggattag aggaaggtca gttacttcca
    16201 ggagtgttag tcgcctttgt gatgtatgtg caaagatttt ttgatcctat tcgcaatttg
    16261 actcagcaat atactcagtt gcaaagatcc atggcttcgg gggcgagaat ttttgatttg
    16321 atggataggg agccggagtt agtagatata gacgatgcta tcgagatgcc cgaaataaag
    16381 gggaaggttg aatttaaaaa cgtcagcttc cgatatgtgg aaggtgagga tgtcctaaaa
    16441 gggataaatt taaaggtaaa tgcgggtgaa accgttgctg ttgtgggacc aactggtgct
    16501 gggaaaacca gcattgtgtc tattttgtcg cgattctacc cagttaagcg ggaagaaggt
    16561 gagatattga tagatgggat cgatatcaga gacgtcaaga gagcttcggt cgtaggccaa
    16621 atgagtatgg ttttacaaga accatacctt ttttctggta cggtgatgga aaatattaaa
    16681 tataatcacg tggatgcaac tgatgaaaaa gccatctctt cggcaaaggt agttggggca
    16741 cacgaattta ttatgcagct tgaagatgga tatgacactt ttctttcaga aagaggagcc
    16801 aatattagtt tgggccaacg ccaattaata agttttgcaa gggccattgt cgatgatccc
    16861 aagatactta tcctagatga ggctacggct agcatcgata gccatacgga attagttatt
    16921 caaacggcct tgagaaaatt gttagaagga cgtactgcta ttgtcatagc ccatagacta
    16981 tctacgataa gaggagcaga taaaatagtt gtgttagagg caggtgaggt ggtcgaggta
    17041 gggaatcacc aagaactaat ggataaagac ggtctctacg cgcatctcta caatatgaat
    17101 tttcagtcga ttgaagattt ataataagtt tttgttttct tgagtagcac ttttcctaga
    17161 cgcaatttgg agtttaatgc ttgcacacct agaaaaattc atactttctt tacaggtagc
    17221 taggttatct actctgaccc cttccggagc tcctcatacg ataccaatat gcttcgcttt
    17281 gtttgataac gacacgat
//
LOCUS       DASCNS010000184         3440 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001458672, whole genome shotgun sequence.
ACCESSION   DASCNS010000184 DASCNS010000000
VERSION     DASCNS010000184.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001458672"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="QF838_06625"
     CDS             complement(<1..636)
                     /locus_tag="QF838_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60390.1"
                     /translation="MSYFENDGQIEKTWSHMLPNCYLNTIFITPEWQATWWKRFKYNS
                     TPLIEIVTLDKEAIGVIPILCEGEEATFIGDSNVYDYMDFPVLKGHGKDFFSIAWDRL
                     KSMDWKSLRLESIPEDSPTLKYLPDIAKLDGFEVDLYESDTTPCMQLPDSWDDYLTGL
                     RKKDRHELRRKLRRLESNTDFKQYSVDITGDSVEKNLEEFFRLMALSSDDKG"
     gene            complement(645..1235)
                     /gene="folE"
                     /locus_tag="QF838_06630"
     CDS             complement(645..1235)
                     /gene="folE"
                     /locus_tag="QF838_06630"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012360936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HJO60391.1"
                     /translation="MTESMDSTTAREVFDSDGIRSAVQEILISIGEDPKREGLIDTPN
                     RVVELYRDIFSGLRSDPEEVLSVFFEENHTDPVAFTDISFNSICEHHLLPFHGYAHIG
                     YIPKGRVIGASKVSRLVDVLAKRPQIQERLTTQIADIVYRCLDASASYVFMSAEHMCM
                     ALRGVKKPDSRIITSAQRGGEQEVKQLIKTISLSKS"
     gene            complement(1232..2287)
                     /gene="thrC"
                     /locus_tag="QF838_06635"
     CDS             complement(1232..2287)
                     /gene="thrC"
                     /locus_tag="QF838_06635"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HJO60392.1"
                     /translation="MGKGIIETYKKFLPINSKTPVFSLGEGNTPLVKSRNVVDEVGCR
                     SLYFKLEGCNPTGSFKDRGMVIAVAKAVEKGIGSIACASTGNTSASAAAYGAYLGLRT
                     TLIVPKGNVARGKLAQAVAYGAHIAMIDGNFDKALEITRELCERHPVELVNSLNPNRI
                     EGQKTASFEIVEELGVAPDHLFIPVGNAGNITAYWKGFKEALESGLSNKLPVMNGYQA
                     SGAAPIVDGAIVEEPHTVASAIRIGNPASWEMALRVRDESNGFIDKVSDDEILSTYRR
                     LASDEGIFCEPASAASLAGLLKRVNAGESFEGETIVCVITGNGLKDPDIANEIKPVSL
                     KEYPAEIESVEEALALS"
     gene            2521..>3440
                     /gene="glyA"
                     /locus_tag="QF838_06640"
     CDS             2521..>3440
                     /gene="glyA"
                     /locus_tag="QF838_06640"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HJO60393.1"
                     /translation="MKLQNTDTEISDAIENEKRRQQDNINLIASENYASFAVLEAQGS
                     VFTNKYAEGYPGRRYYGGCEYVDVVEQLAIDRAKALFDADYANVQPHSGAQANMAAYF
                     ASLEPGDTVMGMGLDHGGHLTHGSQVNFSAKLYNFVPYEVDRELETIDFLEVSKTAKE
                     CKPKLIVAGASAYTKTIDFEKFRQIADSVDAKLMVDMAHISGLVAAGIHPSPVESADI
                     VTSTTHKTLRGPRGGFILGKNEMGKALNSAVFPNMQGGPLEHTIAAKAVAFKEAATDE
                     FIEYQKQVVANAKALAETLQQGGLRLVSGGT"
BASE COUNT          998 a          794 c          674 g          974 t
ORIGIN      
        1 tcctttatcg tcgctgctca aagccattaa acggaaaaat tcctccaggt ttttttctac
       61 tgaatctccg gtgatatcaa cggaatactg cttaaagtca gtattggatt caagtctcct
      121 cagcttgcgt ctcaactcgt gacggtcttt ctttctcagc ccagtgaggt agtcatccca
      181 cgagtcaggt agttgcatac agggggtagt atcagattca tacaaatcta cttcaaatcc
      241 atccagcttg gctatatctg gaagatattt aagtgtagga gaatcctctg gtattgattc
      301 aagacgtaga cttttccaat ccatggattt caaacggtcc catgctatcg aaaaaaaatc
      361 tttcccgtga ccttttagta caggaaaatc catgtagtca tatacattag aatcgcctat
      421 aaatgtcgcc tcctcaccct cacataatat gggaattaca ccgatcgcct ccttatccaa
      481 tgtaactatc tcaattaagg gagtcgagtt gtatttaaat cttttccacc aagtggcttg
      541 ccattcagga gtgataaata tagtgtttaa gtagcagtta ggaagcatgt gtgaccaagt
      601 tttttcaatc tggccatcgt tttcaaaata actcatttca cctcttaaga tttggataaa
      661 cttatagttt ttatcaattg tttaacctcc tgctcccctc ctctctgcgc agatgtaatt
      721 atcctgctat cgggtttctt gacacctcgc aaagccatac acatatgctc tgcagacata
      781 aaaacgtaac ttgcgctcgc gtccaaacat cgataaacaa tatctgctat ttgagttgtt
      841 agtctctctt gaatttgagg tctttttgcc aacacatcaa ccaatcgaga aactttacta
      901 gctccaatca ctctgccttt tggtatataa cctatatgtg cgtaaccatg aaatggcaac
      961 aaatggtgct cgcaaatcga attgaaagaa atatctgtaa aagctacagg atcggtatgg
     1021 ttttcttcaa aaaacacgct tagtacttct tcagggtcag atcgcaaccc ggaaaatatg
     1081 tcacggtaca attccacaac cctatttggt gtatctatta gaccttccct tttgggatcc
     1141 tcccctatcg aaattaaaat ttcctggaca gcagacctga tcccatcgga atcaaaaacc
     1201 tctcttgccg tagtagaatc catcgattca gtcatgataa ggcgagagcc tcctccactg
     1261 attcgatttc agccggatat tccttaagag aaactggttt tatttcattt gctatatctg
     1321 ggtctttaag accgttccca gtgataacac acacaatggt ttcaccttca aaagattcac
     1381 ctgcattaac cctcttcaat aaacctgcaa gcgacgcagc agaagctggt tcgcaaaaaa
     1441 tgccctcatc agatgccaac ctgcgatatg tgctgagaat ttcatcatca ctgactttgt
     1501 cgataaagcc attggattca tcgcgaactc tcaaagccat ttcccaacta gctgggttac
     1561 caatccgtat agcagatgct actgtatgtg gttcctcaac aatggctccg tcaactattg
     1621 gggctgctcc agatgcctgg tacccattca tcacaggcaa tttatttgaa agtccgctct
     1681 caagggcttc cttgaagcct ttccaatatg ctgtgatatt accagcgttg ccgactggga
     1741 taaaaaggtg gtcaggtgct accccgagtt cttcaacaat ttcaaaagaa gcggtttttt
     1801 gaccttctat acggttagga tttagtgagt ttactaactc cacgggatgc ctttcacaca
     1861 actctcgagt aatttctaac gctttatcaa aattaccgtc aatcattgct atatgtgcgc
     1921 catacgcaac cgcttgagcc agtttacctc tggccacatt cccctttgga acaatcaagg
     1981 tagttctaag tcctaaatag gctccatacg cagctgcaga agcactcgta ttacctgtcg
     2041 aggcacacgc tatcgaaccg atgcctttct caacggcctt agctactgcg attaccatgc
     2101 ccctatcttt aaatgaacca gtcgggttgc aaccttcgag tttaaaatac agtgatctgc
     2161 aacctacttc atccaccaca tttctcgatt taacgagagg agtgtttcct tctcccagag
     2221 aaaaaaccgg agtcttggaa ttaataggaa gaaatttttt ataagtttcg atgatacctt
     2281 tgcccatttt aaaaggccgt cacgttcctt ctatatacat ttattttctg cataatctag
     2341 gcaggctcaa tgcctccaag tactctacag gagcaatact tctctagcaa cactattgag
     2401 acaaggtgat ctattataag accaacgaaa ttctaaaggc ttgctcaact catttttttg
     2461 acccgcggtg gattaccatt aagccagtat cttttcttga cattgatgaa ggagtaacaa
     2521 atgaagttgc aaaacactga cacagaaata tctgatgcca tagaaaatga aaagagacgc
     2581 cagcaagata atatcaacct aatagcctca gaaaattatg cgagtttcgc cgtacttgaa
     2641 gcccagggat ctgtattcac aaataaatat gcagaaggct atccaggaag aagatactat
     2701 ggcggatgcg aatacgttga tgtagtggaa caactggcaa tagacagggc aaaggcttta
     2761 tttgatgccg actatgccaa tgtacagcct catagtggag cgcaagctaa tatggctgcg
     2821 tatttcgcgt ctttggaacc tggtgatacg gtgatgggta tgggattaga tcatggaggt
     2881 catttgacac acggcagcca ggtgaatttc tcggccaaac tttataactt cgtaccttat
     2941 gaggtagacc gcgagctgga gaccatagat tttctagaag tgagcaaaac agcaaaagaa
     3001 tgcaagccga aactgatagt agccggtgct tccgcataca ccaaaaccat cgacttcgaa
     3061 aaatttaggc aaatagctga ttctgtggat gcaaaactta tggttgacat ggcccatatt
     3121 tctggtcttg tggcggcagg gattcaccct tcccctgtag aaagtgctga tattgtgaca
     3181 tccaccactc ataaaacctt gagaggtcct agaggtggat tcattttggg taaaaatgaa
     3241 atgggaaaag ccctaaacag cgctgtcttc cccaatatgc aaggaggccc tctagaacac
     3301 accatagccg caaaagccgt tgcctttaaa gaagcagcaa cggatgagtt tatagaatac
     3361 caaaagcagg tagttgctaa tgcgaaagcc ttggctgaaa ccttgcagca aggtggatta
     3421 cgtctggttt ctggaggaac
//
LOCUS       DASCNS010000185         1174 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001461111, whole genome shotgun sequence.
ACCESSION   DASCNS010000185 DASCNS010000000
VERSION     DASCNS010000185.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001461111"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="QF838_06645"
     CDS             <1..1050
                     /locus_tag="QF838_06645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HJO60394.1"
                     /translation="FIDENGVILFAGQSGAESHIYEARIGACGQNKVVDLGGQLTACE
                     FDDSHEQFACIYSTLERSPEVFQGTLSDNSIQMVSDLNSEYFLERIVGETERFSLNRS
                     GLDIESVLLYPAGFNPSKKYPLVVNIHGGPHGLFANSFDITRQLLSSNGYFVLAVNPR
                     GTSTYGKDYMLPVLGDWGGEDYNDIMACLDHVCMQDYIDSERLAVTGYSYGGFMSSWV
                     IGHTDRFKCAVIGAPVTNLASFYGTSDIGVNFGERQMGGTRTEIKKEYDFRSPLSYAE
                     NVSTPALLMHGDSDYRVPISQSEEYFVTLKRLGKIVEFVRFPGQNHGLMRTGNLKMRK
                     EYLTRMLSWINKYTT"
BASE COUNT          347 a          232 c          274 g          321 t
ORIGIN      
        1 tttatcgatg aaaatggagt tattttattt gcaggccaat ctggcgctga atcacacatt
       61 tatgaggcaa gaatcggcgc atgcggccag aataaagtag tcgatctagg agggcagcta
      121 acagcatgtg aattcgacga ttcacatgag cagttcgcat gcatttactc aacccttgaa
      181 aggtctcccg aagtctttca agggacccta tcagataatt caatacagat ggtaagtgac
      241 cttaactctg agtatttttt ggagcggata gttggtgaaa cagaaaggtt ttctttgaac
      301 agatctgggc tggatatcga atcagtatta ctctacccgg cagggttcaa cccatcaaaa
      361 aaatatcctc ttgtagtaaa tattcatgga ggcccccacg ggttattcgc taattccttt
      421 gatataacca ggcagctact ttcatcaaac ggatatttcg ttttggcggt aaacccgaga
      481 ggtacctcaa catacgggaa ggattacatg ctgcccgtgc tgggagactg gggtggcgag
      541 gattacaatg acatcatggc gtgtctcgat catgtgtgca tgcaagatta tattgattct
      601 gaaagactcg cagttaccgg atatagctac ggtggattta tgagttcatg ggtgattggt
      661 cataccgacc gattcaaatg tgctgtcatc ggagctccag taactaatct tgcctccttt
      721 tacgggactt cagacatagg ggttaatttt ggtgagagac agatgggtgg aactagaaca
      781 gagattaaaa aagaatatga ttttaggtca ccattaagct acgcagaaaa tgttagtact
      841 cctgccttac ttatgcatgg ggactccgat taccgggttc caatctcaca gagtgaagag
      901 tacttcgtga ccctgaaaag attaggaaag atagtcgaat ttgttcgctt ccccggccag
      961 aaccatgggc tcatgcgaac agggaatcta aaaatgcgaa aagagtactt aacgagaatg
     1021 ctttcttgga taaacaagta cacgacatga cgaaaggagt tgacttatga ctagcaaatt
     1081 aatagctgat tttctatttg aagttcatat tgccatattg gcgatacacc gtgagaacag
     1141 gtccccacat tcaactccta tatggtacca atat
//
LOCUS       DASCNS010000186         7184 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001482723, whole genome shotgun sequence.
ACCESSION   DASCNS010000186 DASCNS010000000
VERSION     DASCNS010000186.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001482723"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2336
                     /locus_tag="QF838_06650"
     CDS             <1..2336
                     /locus_tag="QF838_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60395.1"
                     /translation="IGPGIGGLKYDYRRPKHPYSDKFMLAGGHNAPVTYALWMVLGEA
                     LYQKHKNTGDRKYLADYSQTLLPIDCIGFRRSKEGMKTILEQTGLTDHPAMAQAQLRG
                     IRALSGHSESTDATNDVNGGPSGIGIATAAGKATFWDIIGASESPKIMAVEGEFAMTS
                     GHSQEMKTQAVAQQVGKRLRVLMSYNNAGIDDELIGGVIQPQYESYRIVDQWTSYGWN
                     VFSVDDANDMGQVVAAFKAMEDTDPSDRRPMILIGKTTKGWWPGAENGQIPGYGNQIT
                     SFQSHPYTFAMNSEYFLALAATFEDRFDVKFKGISDGPISDEKERLIQYKENIDVVMS
                     VLDKDGLGDWIADRLVKIGEQIKDQIPTRIDTSIDPFMDERLDYRTLPLEPQTVTVTN
                     PISGEQKNLEIKMFEEPGVPKGSRRAISEIIKWLNYVTDNRLVVAAADLADSINVEKG
                     SIWGEFDPLTNPAGVRLRSAIQEAGNVSTAIGLVSQSASLDPGKHSGVWALSGTYGAF
                     TPLMYLPVRVWSQQNQDSPFRLGVLNILAGHSGPETAADARTHFGIFSPQVWKLFPKD
                     QVINLNFWDYNDVAPGYLAAAEIAARDPKVGVIVLEVARPDFPVVDRSKLADQDIKAA
                     AKGFYILKDFDTSKPKEGYVVVQGSSCTSNLVAQMEQIASEGHNVRIISAVSEDLFNR
                     QSQEYKDHVLPDSAKYNMMIVSTGTKRMWPVTGVGPLTEEYSLVSDHDDLWLSGGSEG
                     EVLKEAGLDPDTIFQSVSKFARDHSNRMKRQSELLGG"
     gene            complement(2359..3480)
                     /locus_tag="QF838_06655"
     CDS             complement(2359..3480)
                     /locus_tag="QF838_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HJO60396.1"
                     /translation="MYKKFIYIFFISFALVSCQIDSNSDIADNSLTQRGATVTPFVPV
                     FENLDKIVRATVESRLDNFEISRKVDSQLALALTATSIAVFGTATMTNTPQPTSTPTL
                     LPTITTTPSPIPTYTPLPTYTPLPTYTVVPTATSIPATTTATPTLTPSPLPTSTPTST
                     PLPTPTSTITPTPTITAIDVLSQATKKVVKILSGSSGGSGVLVSGNEPSSTLIITNEH
                     VIENKSDMLIELYNGNKHEGQIIFEDKEIDIAIIEINALDLEVFNILLVGPPAVGLEV
                     YALGYPLNENYSVTSGIVSANLYGEDSGIQMVQTDAALNPGNSGGALINKDGALIGIN
                     TSRKEKTGSGRIVTNMGYATLIDEVYQKSPHLFSTDIQK"
     gene            3690..4895
                     /locus_tag="QF838_06660"
     CDS             3690..4895
                     /locus_tag="QF838_06660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HJO60397.1"
                     /translation="MGTFGFSTEKLGNIESYLQEKYIDTNRYVGTLTGIYRNGQLGFI
                     STLGLMDRESRKPIERDTIFRIYSMTKPITSVALMSLYEKGLFQLDDPVSEYIPAFKD
                     LNVYAEGIFGNYKTTYPDREMTIRDLLSHQSGLTYGFMERSNVDAAYRELGIEKESQQ
                     NLQEFVNVLSTIPLEFSPGTAWNYSVSTDVCGYLIEVISGKTLDMFLEEEIFQPLGMA
                     DTGFYVPSSKTWRLSSNYEYREGQEPILVDDANTGSYINPPTLFSGGGGLVSTLDDYM
                     AFCKMVLGRGSLEGHRILSRKTLDLMSSNHLTNGKDLRSCAYGRWSETSYTGVGFGLG
                     FSVLLNPAASQVSGSKGELAWGGAASTAFWIDPLEDMAVVFLTQLLPSSTYNVRRELR
                     SLVYSALSD"
     gene            complement(4892..6172)
                     /locus_tag="QF838_06665"
     CDS             complement(4892..6172)
                     /locus_tag="QF838_06665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJO60398.1"
                     /translation="MEFNDLGLDKRLMAGIRSAGYTTLTPIQNEAIPLSIEGRDLLGL
                     AQTGTGKTAAFAIPLLQKLSVGYTGKIRALVIAPTRELADQIHQHISQLSSGLKTRSI
                     TLYGGVSKNPQDEKLQKGVDIVIACPGRLLDHLRESTIDLSTLDTLVLDEADTMCDMG
                     FLPDIRKIIAHLPSKRQTLFFAATMPSDIRNLAKDILDNPTEVQIGVIAPAKTVAHSL
                     YPITEKLKSQLFFRLIETTATGQVIVFTRTKHRTKRLFAELEKRKYNVAALQGNMSQN
                     KRTQAINGFRKGKFDFLVATDIASRGIDIPEITHVINYDMPNTVDAYIHRIGRTGRAE
                     SLGEAITLSIPEDESMITRIEKILKSQIERKTVNDFSYGTSFDPNRRSIRSQNLKSQS
                     SRNHPRRSSGWKRRKESGTNRGPANTGVRSARSN"
     gene            complement(6200..6276)
                     /locus_tag="QF838_06670"
     tRNA            complement(6200..6276)
                     /locus_tag="QF838_06670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6240..6242),aa:Glu,seq:ttc)
     gene            complement(6374..>7184)
                     /gene="argC"
                     /locus_tag="QF838_06675"
     CDS             complement(6374..>7184)
                     /gene="argC"
                     /locus_tag="QF838_06675"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HJO60399.1"
                     /translation="SAEALKDAINSGIKGVDISADFRIRNLDTYSEWYNVEHPCPELM
                     ESSAYGLPELHRSAIKDCQIAANPGCYPTASILGLAPALQAGIIEPEVIVDAKSGVSG
                     AGRGLSLKIHYSEVNENVSAYGLDGHRHMPEISQELDLLSDQHVNLTFLPHLIPMTRG
                     ILASCYAPLKSSFIETGVDLKSEIKALYRDFYKDKRFTHVVDSPPMTKQTLGSNDCVI
                     YPTVDVRAGRLMVISCIDNLVKGAAGQAIQNMNIMCGLPEDLGLTQLAIYP"
BASE COUNT         2022 a         1395 c         1710 g         2057 t
ORIGIN      
        1 taattggtcc aggaataggg ggtcttaaat atgattacag gaggccgaag catccttact
       61 cagacaaatt tatgctggcg ggaggacata acgctccagt tacatacgcc ctttggatgg
      121 tcctagggga ggccttgtac cagaaacata aaaacactgg agataggaaa tatctggccg
      181 attactccca aacattactt ccaatcgatt gcataggatt tagaagatct aaggaaggaa
      241 tgaaaactat acttgaacaa accggactga ccgatcaccc agcgatggca caggctcaac
      301 taagaggtat acgagccttg tctggccatt ctgagagcac agacgcgacc aacgatgtga
      361 atggaggccc ttccggaata gggattgcaa cagccgcggg taaagctaca ttctgggata
      421 tcataggtgc gtccgagtca ccgaaaatta tggcggtgga aggcgagttt gcaatgactt
      481 ctggtcattc gcaagagatg aaaactcaag cggtagccca acaggttggg aaacggctga
      541 gggttctcat gtcttataac aacgctggga ttgacgatga attaatcggt ggagttatac
      601 agcctcaata cgaatcttac agaatcgtag accaatggac ttcatatggg tggaatgtgt
      661 tttcggtaga tgatgctaat gatatgggcc aagtagtggc tgcattcaaa gctatggagg
      721 atactgaccc ttctgaccgt agaccaatga tcttaatagg caaaacaact aaaggttggt
      781 ggcccggtgc ggaaaatgga caaataccgg gatatggaaa ccagatcaca agttttcaaa
      841 gccaccccta cacttttgca atgaactctg aatacttctt ggctcttgcg gccacttttg
      901 aagataggtt tgatgtgaaa tttaaaggta tttcagatgg accaatttcg gatgaaaagg
      961 aacgtttgat acagtacaaa gagaatatag atgttgtgat gtcagtgctg gacaaggatg
     1021 gtttaggaga ctggatagct gacaggttgg tgaaaattgg ggagcagatc aaggaccaaa
     1081 tcccaactcg aatcgatacc agtattgatc cctttatgga tgaaagatta gattatagaa
     1141 cccttccact tgaaccccag acagtaactg taacaaaccc tatctcaggt gagcagaaaa
     1201 atctggaaat caaaatgttt gaagaacccg gagtgccgaa aggaagcagg agggcgatat
     1261 cagaaataat taaatggctc aattatgtaa ctgataatcg gctagtcgta gcggctgctg
     1321 accttgctga ttctatcaac gttgagaaag gctcaatttg gggagaattt gacccactca
     1381 caaacccggc cggagttcgc ctccgatctg caatccagga ggccgggaat gtatcgacag
     1441 cgattggatt agtaagtcaa tcagcctcac tcgaccctgg aaaacattcc ggagtctggg
     1501 cactaagtgg tacttatggt gctttcacac cacttatgta cttgccggtg agggtctgga
     1561 gccagcaaaa ccaagatagc cccttcagat taggagtttt gaatattctt gcaggtcact
     1621 ctggccctga aactgcagct gatgctcgca cacattttgg tatattttcg ccacaagtat
     1681 ggaaactatt tcctaaagat caagtgataa accttaattt ttgggactat aacgatgtag
     1741 cccctggata tttggcagcg gcagaaatag cggccagaga tcccaaagtg ggagtcatag
     1801 ttttagaggt ggcaaggccg gatttcccgg tagtggatag gagtaaattg gctgatcagg
     1861 atatcaaggc agcggccaaa ggattttaca tattgaagga tttcgataca tctaaaccta
     1921 aagaaggtta cgttgtagta caaggctctt catgcactag taatctggtg gcccaaatgg
     1981 aacagattgc atctgaaggt cataacgtcc gcatcatatc agctgtttca gaagatctat
     2041 ttaatcggca atctcaagaa tataaggatc atgtactgcc tgattcggcc aagtacaata
     2101 tgatgattgt aagtactgga accaagagga tgtggcctgt tacaggagtt ggacccctaa
     2161 cggaagaata ttcattagtg tcagatcacg atgatttgtg gctaagcgga gggtccgaag
     2221 gagaagtttt gaaagaggca ggtttggatc ccgacacaat ttttcaatcg gtttccaagt
     2281 tcgccaggga tcattctaat aggatgaagc gccagtccga gctgttaggc ggctagagat
     2341 agggtaatag tgttattgtc acttttgaat gtccgtacta aaaaggtgcg gacttttttg
     2401 atagacctcg tcaattagag ttgcatatcc catattggtc actatccgcc cggacccagt
     2461 tttttccttt ctgctggtat taataccgat caaggcacca tctttgttga tcaaagcccc
     2521 tccgctatta ccagggttta gagccgcgtc agtttgtacc atctgaatac ctgaatcttc
     2581 tccataaagg ttggctgaga ctatccctga agtaacggag tagttttcat ttaatggata
     2641 acccaaggca taaacttcca aaccaaccgc agggggaccg acaaggagta tattaaatac
     2701 ttctaaatcc aaagcattta tttcaataat tgctatatct atctctttat cttcaaatat
     2761 aatttgccct tcgtgtttat ttccgttata gagttctatt agcatgtcag atttattttc
     2821 gatgacatgt tcattagtga ttattaacgt tgatgagggt tcgtttcctg acactagtac
     2881 cccacttccc ccgcttgatc cagataggat tttcactacc ttttttgttg cttgagataa
     2941 cacatctatt gcggtaattg ttggggttgg tgttattgtc gatgttgggg tcggtagagg
     3001 cgtggaagtt ggagtactgg taggtaaagg agagggtgta agggtcgggg ttgcggtggt
     3061 tgtcgctggt atggaagtag cggtgggaac tacagtatag gtaggaagtg gcgtataggt
     3121 aggaagtggc gtataggtag ggataggact cggggtagtg gttattgtag gaagaagagt
     3181 tggtgtagaa gtcggttgag gagtgttggt cattgtggct gtaccaaata ccgcgataga
     3241 tgttgcagtc aaagcaaggg ccagctgact gtcaacctta cggctaatct caaaattgtc
     3301 taatctggac tccacagttg ctctaacaat tttatctaag ttttcaaaaa ctggtacaaa
     3361 cggagtaacc gttgcacctc tctgtgtaag ggaattatcg gcaatatccg agtttgaatc
     3421 gatctgacat gagaccaaag cgaagctaat gaaaaatatg tatatgaact ttttgtacaa
     3481 ttcgagtact cgtaaagggt ttgttataaa aatggatcaa ccgaaacact ataggtagta
     3541 ttgtcacctg agggacttat gaaagtctac aggaggttca agtttggagg ggcatcttaa
     3601 tgggaaagat gaaacaatat ggggatgtat tacattaaag taaggaacaa ttaaccttca
     3661 aataccaatg aaatagaggt caaacttaca tgggcacctt tggattctca acagagaaac
     3721 tgggaaatat tgaatcttac ttacaggaaa aatacataga cacaaatagg tatgtcggga
     3781 ctcttactgg aatatatagg aacggccagc taggttttat atccacccta ggattgatgg
     3841 atagggaatc tcgtaaaccc atcgaacggg atactatatt tcgaatatat tcaatgacaa
     3901 agccaataac ttctgtagct ttgatgtctt tgtatgaaaa gggattgttt cagttagacg
     3961 atcctgtctc agaatatatc ccagccttca aagacctgaa cgtttatgca gaaggaattt
     4021 tcggaaacta caagacgact tatcctgata gggaaatgac aataagggat ctacttagtc
     4081 atcaatctgg attaacttac gggtttatgg aacgatcgaa tgttgacgct gcatatcgag
     4141 aacttggaat cgaaaaggag tcacagcaaa accttcaaga attcgtgaat gtgttaagta
     4201 ccatcccatt ggaattttca ccgggtacag cttggaatta ttccgtttct actgacgttt
     4261 gtggctactt gatagaagtg atatcaggta aaactttaga catgttttta gaagaagaaa
     4321 tattccaacc tttagggatg gccgatacag gattttacgt tccatcctct aaaacctggc
     4381 gcttgtcgtc caattacgaa tatagggaag gacaagagcc gatattagtc gatgacgcta
     4441 atactgggtc ttacattaat ccccctaccc tgttttctgg aggaggaggt cttgtgtcaa
     4501 cattggatga ctatatggcg ttttgcaaaa tggttttagg aagagggagc ctagagggac
     4561 acaggatatt aagccgaaaa accttggatt tgatgagctc taatcatctt acgaacggaa
     4621 aggacttaag gagttgtgcg tatgggaggt ggagtgaaac ttcatacacc ggggtcgggt
     4681 ttggtttggg attttcagta ttgctgaacc cagcagcctc acaggtctct ggcagtaagg
     4741 gtgaattagc ttggggtgga gccgctagca cagctttttg gatagatcca ttagaagaca
     4801 tggcagtggt ttttctgacc cagttactac cttccagtac atacaatgtt cggagagagt
     4861 tgcgttcgtt ggtgtattcc gctttatcag attaattaga tcgggctgat ctcaccccag
     4921 tatttgcagg gccacgattg gttccagatt ccttccgcct tttccaaccg gatgaacgcc
     4981 taggatgatt tcttgaactt tgcgatttta aattctgact gcgaattgac cgtctgttgg
     5041 gatcaaacga agttccatag ctaaaatcat taacggtttt tctttcaatc tgtgatttca
     5101 ggattttttc aattctggtg atcattgatt cgtcttcggg tattgaaaga gttatggctt
     5161 caccaagact ttctgctctt cctgtgcgcc cgatcctatg tatataagca tctacggtgt
     5221 ttggcatatc gtagttgatt acatgtgtga tttctggaat atcaataccg cgggaggcta
     5281 tgtcagtagc cactaaaaag tcaaatttac ctttccgaaa cccatttatg gcttgagtcc
     5341 gtttattctg cgacatatta ccttgaaggg ctgctacatt gtattttctt ttttctagct
     5401 ccgcgaatag ccttttggtt cgatgttttg ttctggtgaa tactattact tgacctgtcg
     5461 ctgtggtttc tattagtcgg aaaaacaact ggcttttaag cttttctgtg atcggataaa
     5521 gagaatgagc aactgttttt gctggagcta taacccctat ttgaacttca gttgggttat
     5581 ccaaaatatc tttggccaaa tttctaatat cacttggcat agtagcggca aaaaataagg
     5641 tttgcctttt ggatggtaaa tgagcgatta tctttcgaat atcaggaagg aaacccatgt
     5701 cgcacattgt gtcagcttca tccaacacca atgtgtctaa agttgacaaa tcgatagttg
     5761 attctctgag gtgatccaat agacgacccg ggcaggcaat tactatgtct accccttttt
     5821 gtaatttttc atcttgagga tttttagaaa ctcctccata caaggtaatg gatctggtct
     5881 tcaacccaga gctcaactga gatatatgtt ggtggatttg gtcagctaac tctctggtag
     5941 gggctattac taaagctctt attttgcctg tatagccaac tgacaatttt tgcaatagtg
     6001 gaattgcaaa agcagcagtc tttccagttc cggtctgtgc caatcctaat aaatcccggc
     6061 cttctattga aagtgggata gcttcattct gtattggggt aagagtcgta tagccagctg
     6121 atcgaatgcc ggccattaga cgtttgtcga ggccgaggtc gttaaattcc aaaaagaacc
     6181 ttcctaaaat aaatggtcat ggtgacccca gcgggattcg aacccgcgat ctccgccttg
     6241 aaagggcggt gtcctagacc gctagacgat ggggccatgt tagtttcgaa aaatcgtctg
     6301 ttgtttaagc aacactaaat agtctatgtc ctcggttggg atagagcaac gattatttag
     6361 atggaaaata accctatgga tatatcgcca attgggttaa tcccaagtct tcaggcagac
     6421 cgcacatgat attcatgttt tgaatggcct ggcctgcagc acccttcacg agattgtcta
     6481 tacaactgat taccatcaat ctacctgccc tcacgtcgac cgttggatat atgacgcaat
     6541 cattacttcc aagagtttgt tttgtcattg gcggagaatc gacaacatga gtaaatcttt
     6601 tatctttgta aaagtccctg taaagagcct ttatttctga ttttaggtct actcctgttt
     6661 cgataaaact gctctttaaa ggtgcgtagc aagaagctaa tatacctcgt gtcattggga
     6721 tcaggtgagg taggaaagtg aggttgacat gttggtcaga tagtaaatct aattcctgtg
     6781 aaatttctgg catatgtcga tggccgtcca gcccatatgc actcacgttt tcattgactt
     6841 cggaataatg gattttgagg gagagacccc tgccagctcc tgatactcca gatttagcat
     6901 caacgatgac ttctggttct ataattcctg cctgtaaggc gggtgcgagg cctaatatgg
     6961 aggcagtcgg atagcacccg gggttggcag cgatttgaca gtctttgatc gcagaacggt
     7021 gcagctcagg taaaccatat gcactggact ccatcaattc tggacaaggg tgttctacgt
     7081 tataccattc cgaataagtg tcgagattcc gaatgcggaa gtccgcgctg atatctacgc
     7141 cttttattcc tgaattgatt gcgtccttta aagcctctgc actg
//
LOCUS       DASCNS010000187         9799 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001496854, whole genome shotgun sequence.
ACCESSION   DASCNS010000187 DASCNS010000000
VERSION     DASCNS010000187.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001496854"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="QF838_06680"
     CDS             complement(<1..917)
                     /locus_tag="QF838_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012515.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HJO60400.1"
                     /translation="MQKFITFGETMVQYNAEYTGSFDPKKRYFPDVAGAESNVAINLS
                     KLTENHAEIFWVSRLGRDDSGRLIQKILSQHINIDAPICFGEKTGISYLNHHANGQHV
                     KTYFRKGSAASEIPFEQVKKHLKKADMLHITGITPALSNTNLETVVKSLEFCNEMDIP
                     VCLDVNYREQLWNPTEAKNTLEKLLPFISIFKVGHDEAETIWHLNLSAEDYAANFYKK
                     PGTLTIVTDGESGAVLFNGTNYIREPSIKIEVIDPVGAGDAFVAGFLGTLIRQNIPLR
                     DFLKAEHEGQIESLTKALRVGNICGALTCT"
     gene            complement(952..1710)
                     /locus_tag="QF838_06685"
     CDS             complement(952..1710)
                     /locus_tag="QF838_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO60401.1"
                     /translation="MSNLRQNKAKKNLSDGKIVSVPMGPMNGDLIEHFGPLGFDAMWL
                     EGEHGPVDFNNIPDLTRACDLWDMTPIVRVHQNEPATIYRTFDLGAQAITVPHINTKE
                     EALSVVDSGKFAPIGNRGSFTSRQGIGVDDYFNQANDQTMLIILIEDIIAVNNLDEIL
                     EVENIDVFFVAPGDLAQTMGYPGGAGRREVKEVVEGAIKKISSSGRTAGTLAGTDAVG
                     RYIDIGARFFSFSYLPWLAEGAEKFHETVTKATE"
     gene            complement(1736..2701)
                     /locus_tag="QF838_06690"
     CDS             complement(1736..2701)
                     /locus_tag="QF838_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014841.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HJO60402.1"
                     /translation="MPKFVFMPPQDDLKREFAARLSDTLPEYDVHSPETDEDAIRVIK
                     DADAAMGWIPPEALEVASKIQWLHNPDAGPFFGYYYEDLCKHSLTITNPRGIYWDHIS
                     HHILMFILALSRGLPWYMDAKRERIWDKDARKTPYVFLGDATALINGVGGIGHETARL
                     CRELGMRVIGIDPRQEYKIEGVEMYQPESLAELLPQADFVITTVPHTPETEFTFNKDT
                     FGLMKKTSYFINIGRGMVCKLDDLAEAVEKGLIAGCGLDVYETEPLPSEHKLWGLDNV
                     LMTPHIAVADAENLSERRYQILIENARLFLDNDGLNNIVDKEKWF"
     gene            2795..3628
                     /locus_tag="QF838_06695"
     CDS             2795..3628
                     /locus_tag="QF838_06695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60403.1"
                     /translation="MTVVDTHTHAGVNWFEPVEMLLHQMTLNQVDHAVLIQHGRPEYG
                     TYDHSYLYECVERFPGKFNIVVIVDSDKKDSLRKLEEHKEKGAVGVRLTATTRSPGPD
                     QFAIWRKAAELGMVVSCMGTVDDFSSDQFAELVAEFPTIPIIIEHLAGGGEGSAFPRN
                     GKAPVSPFSKYKKAMQLGDFGNTYLKIHGLGEFNIRPNALKEKFDRNFFDDIPPLLEM
                     AKDAFGYKKIMWGSDYPPVSGREGYRNAMKTAMDNPVFPEPTEIEWIMGGTALTLFNF
                     V"
     gene            3672..4823
                     /locus_tag="QF838_06700"
     CDS             3672..4823
                     /locus_tag="QF838_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60404.1"
                     /translation="MASSVAVGGSQYSFGHFVEPLENTFGWTRTQIGLSLSLIAFGSF
                     ISPLIGSLIDKQGSRRIMAFSMAVFGISYLVRPFMTELWHWYFLSVVQSFSMVGAAML
                     PPGKLVGLWFPENRGRVIGITAMGNNFGGMVIQPIIAFLVTVYNWKVAYAVVGALGIL
                     VSVYSYLVVKNPDSESKGILESERNTDNLTGFTLKEAIRTKTFYAITFAILCGTFTYS
                     SLLPQVSSHLIINGVRESTASIAVSLFATCGMVGKFVFGNLSDKYGPRMALVLDLCGQ
                     AIFASLLVYAGDGLPVWVIVPAMGFFLGAFGALYQLIVIDAFGVKHFGAIMGVIAISN
                     AVPSFLGPIIAGMSFDITGSYAVAFVITSVIFVLGAISLKFTRENGTLG"
     gene            complement(4846..5589)
                     /locus_tag="QF838_06705"
     CDS             complement(4846..5589)
                     /locus_tag="QF838_06705"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HJO60405.1"
                     /translation="MKSFNKESIVWLGVYGHPDDETSASGGTMVKLSDKGHQVYVITA
                     TGGELGTLGTDGSKIKREDLASVRECELKTNMKAYGANPPFMLRYVDQELEKEDPEVL
                     AIKILDIMHMVEPDVVSTFGPSGISNHPDHIAIHKSTLIAYEKYTNPTGENTPLLIYP
                     SIPEDIAKMYNLQLSPVEKNMDLIIDIDDTIRYKIEGLKNYKSQEDAQEFAAHLSERE
                     GPHFESFAVSDSSQNDWESHPVVRYLRSL"
     gene            complement(5576..6226)
                     /locus_tag="QF838_06710"
     CDS             complement(5576..6226)
                     /locus_tag="QF838_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HJO60406.1"
                     /translation="MHVKDLPYQTFPSIFSSIDSFTKNVISAFFEKFSRLPDWLEEIG
                     EVTENIKPPNWRKFYRDDENTGIRVRGEADAIFRLSDGSYVIADYKTSRYNPERTFAL
                     LSYQMQLNAYAWIAESVGYSPISRLYLIFMEPESQKEFASSNSSTSEMGIALGFKSNI
                     IEVERRPEKLIPPILDLVHSVSKMKIPPKGRNRCRDCVRLDEMIVKMTYGITNEIV"
     gene            6371..7237
                     /locus_tag="QF838_06715"
     CDS             6371..7237
                     /locus_tag="QF838_06715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO60407.1"
                     /translation="MEKRRLGRTGHSSTVITFGAYSIGKLSQPEADSAIQMCLDYGVN
                     HIDIAPGYSESMERVAPWMPDLRSKMFLGCKTPMRTRDDVWSNVQDIMGRMNVDSFDL
                     FQLHSVIDLETLDLVTGKGGALEALIEMQEQGLTKYLGITGHGPSSPATHLEALRRFN
                     FDTVMFPVNASMYRNPEYQKDSDALIQFCNQNDVGIQTIKMIARGGWADKEKDCATWY
                     DPYREQKEIDEALWWQLSQKIDTAPSCGEFSLLEKVLDAGSRFQQLSTEAQENITSTR
                     VSIDPEPKLAII"
     gene            complement(7234..8502)
                     /locus_tag="QF838_06720"
     CDS             complement(7234..8502)
                     /locus_tag="QF838_06720"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60408.1"
                     /translation="MRNTEDSPNIKPWSAFRFPNFRMLWVSGLCASVTMQIRLLGFGV
                     WLYEETGSGIQLGLLGLVQLAVQMPASLFGGAFADQFDRKKLIAITQCFSFFLIALAT
                     ILLITDSLKPWHIYAMVAILGVTSTMGGPARSAITANVVPTSHLLHAVTSNTATYQIS
                     SILTPLLFSLAYKLVGLPAVFLIGVCFSLPASIIPLFINANYGTDGSDKPSEGSMTSR
                     LWEGFTFVRNHPILPGLYIMDIGVTVVSFYREIMPLIVDKLFKQGAWAIGPLSAANSL
                     GGVCGSFLVLFLAKYRSKGMLVLYATGVYSLLLFGFGSIQFLNVHPLFLLLIGGGIIS
                     GLGATDAIGMTTRQTTVQLTTPDHMRGRAVSFHSFCAMAANNIGTFEVGFMSERIGAG
                     NTMLLGGTVSVCVVLLVWRLITGLRNYRYP"
     gene            8672..9274
                     /locus_tag="QF838_06725"
     CDS             8672..9274
                     /locus_tag="QF838_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016211.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HJO60409.1"
                     /translation="MVARLWVLFPICIGLSLAIVGVEGLYEPGSISFGIYDWGPRLVG
                     SNDWVYCSFIPSGLLITGYFLVGMSALKDNPLARIFKKVVLYIFGFLFLGIGIAGYFL
                     PMLPGTIFLILSAVCFMRSNEKMYRWVTEHRLFGRHVKQFMETGAMPKRAKFISIGCI
                     WFFSAISTSPITPYGWQFKAIVVSLAVIGTFYILTRPNSK"
     gene            complement(9285..9563)
                     /locus_tag="QF838_06730"
     CDS             complement(9285..9563)
                     /locus_tag="QF838_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HJO60410.1"
                     /translation="MCTMICERTEVEGSGKGRDGWFPLEMVNVSYDHPFNAPWEYGVN
                     IDFVNQSRGVGSRVAVELSPQSAKKLADTIIEALKRGEANPQIKVSVL"
     gene            complement(9585..>9799)
                     /locus_tag="QF838_06735"
     CDS             complement(9585..>9799)
                     /locus_tag="QF838_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HJO60411.1"
                     /translation="GIFGDLDQSPTPEQVNQHEAELKAAGKDIEFHRYPEAGHGFFYF
                     DRPIYHQSSAVDGWDKMLTFLSRTLS"
BASE COUNT         2611 a         1994 c         2172 g         3022 t
ORIGIN      
        1 gtgcaagtga gggccccaca aatattccct actctcaatg ctttagtcag tgactctatc
       61 tggccttcat gctcagcctt gaggaaatca cgcaagggaa tattttgcct aattaaggtg
      121 ccgagaaaac cggcaacaaa cgcgtcgccg gcaccaacag ggtctataac ctcgattttg
      181 atgctgggtt cacgaatgta gttggttcca ttaaaaagta ctgcgccaga ttcgccgtct
      241 gtcacgatag ttagcgtacc gggtttttta tagaaattag cagcatagtc ctcggctgaa
      301 aggtttaggt gccagatcgt ttctgcctca tcgtgcccga ctttaaatat agatatgaat
      361 ggcaaaagtt tttctaatgt gtttttggct tcagttgggt tccaaagttg ctcccgataa
      421 ttaacatcta gacaaacagg gatatccatt tcattacaga attctaggga cttaaccaca
      481 gtttccaagt ttgtattact gagtgctggt gtaatccctg taatatgcaa catgtccgct
      541 ttttttaagt gctttttcac ctgctcgaac ggtatctcac ttgccgcact tccttttctg
      601 aaatacgttt ttacatgttg accgtttgca tggtggttta gataggaaat tcccgttttc
      661 tcaccaaaac aaataggggc gtcaatgttg atatgttgtg aaagaatttt ttggattagc
      721 ctacctgaat catctcttcc gaggcggctg acccaaaaaa tttcagcgtg attttcggtt
      781 aatttagaaa ggttaattgc aacatttgat tcggcacctg ccacatcggg gaaatatctt
      841 ttcttggggt cgaatgagcc agtgtattcg gcgttgtatt ggaccatagt ttccccaaaa
      901 gttataaatt tttgcacggg tgcttccctt gaaaaattag tatggagttt tttattcggt
      961 cgcttttgtt acagtttcgt ggaatttttc tgctccttcg gctaaccatg ggagataact
     1021 gaatgagaaa aatcgggctc cgatatctat ataccttccg actgcatcgg ttccggctag
     1081 tgtgcctgca gttctacctg acgaactgat ttttttgatg gcaccttcca caacttcctt
     1141 tacttccctc cggcccgctc ctccgggata gcccattgtt tgggctaaat ctcctggggc
     1201 tacaaagaac acatcaatgt tttcaacttc gagtatttcg tcgaggttat tgaccgcaat
     1261 aatatcttcg atcaagatga tcagcatggt ttgatcattt gcttggttga aatagtcatc
     1321 cactcctata ccttgcctac tggtgaaaga acctctgttc cctatcggcg cgaatttacc
     1381 ggaatccact actgaaagtg cctcctcttt ggtattgatg tgaggaactg tgattgcttg
     1441 ggcaccaaga tcgaaagttc tatagatggt ggccggttca ttttggtgaa cccgcacgat
     1501 aggtgtcata tcccagaggt cacaggctct tgttaagtca ggtatgttgt tgaaatctac
     1561 aggaccgtgc tccccttcta gccacattgc gtcaaatccc aaaggaccaa agtgctctat
     1621 taaatctcca ttcatcggtc ccatagggac tgacactatt ttcccatcgc ttaaattctt
     1681 ttttgcttta ttttgtctca agttactcat tgggttcctc tcgtttgctt ggttattaga
     1741 accatttctc tttatcaacg atattattca gtccatcgtt atccagaaat agccgggcat
     1801 tttctatcag tatctgatat ctacgttcgg ataagttctc tgcgtctgcc actgcaatgt
     1861 gaggcgtcat gagaacgttg tctagacccc acagtttatg ctctgaaggt aaaggttctg
     1921 tttcataaac gtcaagtccg catcccgcaa taagtccttt ttccaccgct tcagccaggt
     1981 catctaactt acagaccata cctctaccta tatttataaa atacgaggtt ttcttcataa
     2041 ggccgaaagt atctttattg aatgtaaact ctgtctcagg tgtgtgaggc actgtggtga
     2101 tcacaaaatc agcttgagga agaagttcag caagagattc aggctggtac atctctacac
     2161 cttcaatttt atattcttgt ctcgggtcaa ttccgattac ccgcatccca agctcgcgac
     2221 atagccttgc cgtttcatga cctatcccac ctactccgtt gattaaagca gtagcatctc
     2281 cgagaaaaac gtaaggtgtt tttcgggcat ctttatccca gatccgttcc ctttttgcgt
     2341 ccatatacca tgggagtcct cgagataaag ccaatataaa cattagaatg tggtgggaga
     2401 tatgatccca atagattcct ctaggatttg taatcgttag ggaatgctta cagagatctt
     2461 cgtagtaata gccgaaaaag ggtccggcgt cgggattgtg tagccattgg attttggacg
     2521 caacctcaag agcttcagga ggtatccatc ccatagctgc atcagcatct ttaatcactc
     2581 ttatagcatc ctcatctgtt tcaggggaat gaacatcata ttcgggcaaa gtgtcagaaa
     2641 gtctggcagc gaactcacgt ttaagatcat cttggggcgg cataaaaaca aattttggca
     2701 tatcctactc ctagtattcc ttgatgtgaa cctggaaact taatgaggat ataacactta
     2761 ccaaaatcgg taaattgata ggctggagat tttaatgacc gtagtggata ctcacaccca
     2821 tgccggagtt aactggtttg aacccgtgga gatgttgctg caccagatga ccctgaatca
     2881 ggtggaccat gctgttctaa tacaacatgg cagaccagaa tatggaacat atgatcatag
     2941 ttacttatac gaatgcgtcg aaaggtttcc agggaaattt aacatagtcg tcattgtaga
     3001 ctctgataag aaggactcat tgaggaaatt ggaggaacat aaagaaaaag gagctgtggg
     3061 tgttcgcctc acagccacca cccgttcccc cggaccagac caatttgcta tttggcggaa
     3121 agctgctgaa cttggaatgg ttgttagttg catgggtacc gtagacgatt tctcatctga
     3181 tcaatttgca gagcttgtag ctgaatttcc cacaattcct ataataattg agcatttagc
     3241 agggggggga gaagggagtg cattcccacg gaatgggaag gctccggttt ctccgttttc
     3301 taaatataaa aaggctatgc aattgggtga ttttgggaac acatatttaa aaattcatgg
     3361 actaggggaa tttaatatta gaccaaatgc tttaaaggaa aaattcgaca ggaatttttt
     3421 tgatgatatc cctcccctgt tagagatggc gaaagatgct tttgggtata aaaaaattat
     3481 gtggggtagt gattaccctc ccgttagtgg cagggaaggt tatagaaatg caatgaagac
     3541 agctatggac aatcctgttt tcccagaacc cacagaaatt gaatggatta tgggcggaac
     3601 tgctctaact ttgttcaatt ttgtataaca ttaattttca tcgaggctgg gctatcgctg
     3661 cagcgtgctt tttggcttct tctgttgccg taggtgggtc tcaatactca tttggccatt
     3721 tcgttgagcc tttggaaaat acttttggtt ggacacggac ccaaataggg ctgtccttgt
     3781 ctttaatcgc atttggatcg tttatatcac cattgatcgg gtccctgata gataaacagg
     3841 gatcaagacg aataatggcc ttttctatgg ctgtatttgg cattagttat cttgttagac
     3901 cgttcatgac agaactgtgg cattggtatt tcctaagtgt cgttcaatct ttctccatgg
     3961 tcggagccgc aatgttgcct cctggtaaat tggtcgggtt atggtttcct gaaaatcgag
     4021 ggagagtaat cggcataact gcgatgggaa ataatttcgg cggaatggta atacaaccaa
     4081 ttattgcatt tttggttacc gtatataact ggaaggttgc ttatgcagtt gttggggctt
     4141 tggggatttt ggtatccgta tattcttatt tggttgtaaa aaatccagat tccgaaagta
     4201 aaggaatatt agagtcggaa aggaacaccg acaatttgac tggatttact ttgaaagagg
     4261 caattcgcac aaaaactttt tatgctatta cctttgcgat actatgcggg acatttactt
     4321 atagttcatt gttaccccaa gtttcttctc atctcattat taatggggtg agagaaagca
     4381 ctgcatccat agcggtaagc ttgtttgcta cctgtggaat ggtgggcaaa tttgtcttcg
     4441 gtaatttgtc cgataaatat ggtccacgca tggccttagt tttagatttg tgtgggcaag
     4501 caattttcgc ctcgctgctt gtttatgcag gagatggact tccagtgtgg gtaattgttc
     4561 ccgctatggg gttttttctg ggggcctttg gagcccttta tcaactaata gtgattgacg
     4621 catttggggt gaaacatttt ggtgcaataa tgggggtcat agcaatatcc aatgctgtac
     4681 cttcatttct gggtcccatc atagcgggaa tgagtttcga tatcactggt agctatgcag
     4741 ttgcctttgt catcacgtct gtaatatttg tgcttggtgc aatatcactg aaattcacgc
     4801 gggaaaatgg tactttgggc tgatgctaag gggaagctac tttttctata atgagcgcag
     4861 atatctcact actggatgtg attcccagtc gttttgggaa gagtcactaa cggcgaatga
     4921 ttcaaaatgg ggaccttctc tttcagaaag gtgtgctgcg aactcttggg catcttcttg
     4981 gcttttataa ttttttaggc cttctatttt gtacctaatg gtgtcatcaa tatctatgat
     5041 caagtccata ttcttttcaa ctggacttaa ttgtagattg tacattttcg ctatatcttc
     5101 cggaatggaa gggtagatca gaagcggagt gttttcccct gtaggattag tatatttttc
     5161 ataggcaatc agagtggatt tatggatggc tatgtgatcc ggatggttgg atatgccact
     5221 tgggccgaag gtcgatacaa catctggttc aaccatatgc attatgtcta gaatttttat
     5281 cgctaacacc tcagggtctt ctttttccag ttcctggtct acgtaccgca acataaaggg
     5341 tggatttgcc ccgtaggctt tcatgtttgt ttttaattcg cattctctaa cagaggcaag
     5401 atcctccctt ttgatcttgc taccatcggt tcccaatgta cctaactccc ctcccgtggc
     5461 tgtgataacg tagacttggt ggcctttgtc acttagcttt accatagtgc caccactggc
     5521 agaggtttca tcatctgggt gtccgtacac tcccaaccaa acgatgcttt ctttattaaa
     5581 cgatttcatt ggtaattccg taggtcattt taacaatcat ttcatcgagc ctcacacagt
     5641 cccggcatct atttcttcct ttaggaggta ttttcatttt ggagacagaa tggactaaat
     5701 ctaatatcgg cgggatcagt ttttctggtc ttctttcaac ttcaattata ttggatttaa
     5761 atccgagagc tattcccatc tctgaagtag aggaattaga tgatgcgaac tctttttgac
     5821 tttccggttc cataaatatc aaatagaggc gactgatagg gctgtatccg acggattccg
     5881 ctatccacgc ataagcattt agctgcattt gatacgataa cagggcaaat gttctttcag
     5941 ggttgtagcg tgaggttttg taatcggcaa tcacatagga cccgtctgaa aggcgaaata
     6001 ttgcgtctgc ttcccctctc acccttatcc cggtgttttc atcatccctg taaaattttc
     6061 tccaatttgg aggttttatg ttctctgtga cttctccgat ttcttccaac cagtcaggta
     6121 accgggaaaa tttttcaaaa aatgcagaaa tgacgttttt agtaaaactg tctatcgaac
     6181 taaatataga agggaatgtt tgataaggaa gatccttgac gtgcattctt atccaggcac
     6241 accttaagca aggatatcgt gttgcatagt tgcctaattc agttgccgag attgtaatag
     6301 ccattttatg tatgtggtta tactagattg aatatatttc gggacattat tacaaaaaaa
     6361 gaggctggtt gtggaaaaac gtcgacttgg gagaactgga cacagcagta cagtaattac
     6421 cttcggagcc tattcgatag gaaaactaag tcagccagag gcagattctg ccatccaaat
     6481 gtgtttggat tacggagtca atcatataga tatagcacct ggatactcag aatcgatgga
     6541 acgtgttgcg ccatggatgc cggatttacg ctccaagatg ttcttgggtt gtaagactcc
     6601 aatgagaact agggatgatg tgtggagcaa tgtgcaagac ataatgggtc gtatgaatgt
     6661 ggactctttt gatttgttcc aattgcattc ggtaatagat cttgaaactc tagatctcgt
     6721 gacaggaaaa ggtggggcct tggaggcgtt aatagagatg caggaacaag ggcttacgaa
     6781 atatcttggt attactgggc atggcccttc ttcaccggct acacatctag aggcattgag
     6841 aaggtttaat tttgatactg tgatgtttcc ggtgaatgct tcgatgtata gaaatcctga
     6901 gtaccagaaa gattcagatg cattaattca attttgcaat caaaatgatg taggaatcca
     6961 gacaataaag atgattgcca gagggggatg ggctgataaa gaaaaagact gtgctacctg
     7021 gtacgacccc tatagggaac agaaagaaat agatgaagct ttgtggtggc agttatcgca
     7081 aaaaatagat acggccccta gctgtggaga gttctcatta cttgaaaaag ttttggatgc
     7141 tgggtcacgg tttcaacagt tatcaacaga ggcccaagaa aacataactt caacaagggt
     7201 ttccattgat ccagagccca aattagcaat aatttaaggg tatctgtaat ttcttaaccc
     7261 agttattagg cgccatacaa gaaggaccac gcaaacagat actgttccgc ctaatagcat
     7321 agtgtttcca gctccaattc gttcggacat gaagccaacc tcaaatgttc ctatattatt
     7381 tgctgccatc gcgcaaaaag aatgaaaact tacagctcta cctctcatat ggtcaggagt
     7441 tgttaattgc accgtagttt gtctggtagt catacctatt gcgtcagtcg cacccagtcc
     7501 ggaaattatt cctcccccga ttaataatag aaacaacggg tgtacgttga ggaactgaat
     7561 tgagccgaat ccgaataaca aaagggaata tactcctgta gcgtatagaa ccagcattcc
     7621 tttggagcga tatttggcca agaaaaggac aaggaaactt ccacaaactc cgcccagaga
     7681 attggcggct gagagaggtc ctatagccca ggcaccttgc ttgaataact tatcgactat
     7741 taaaggcatg atttctctgt aaaagcttac gacggtaacc cctatgtcca tgatatatag
     7801 accaggaagt atgggatggt ttctaacaaa tgtgaatcct tcccacaatc ttgaagtcat
     7861 agagccttct gaaggtttat cagatccatc ggtaccgtaa ttggcattaa taaacaacgg
     7921 tattattgag gctggtaggg aaaaacacac gcctattaaa aatacggcag gtagtccgac
     7981 taatttatat gcaagggaaa ataataaagg tgtcaggatg gagcttattt gatatgtagc
     8041 tgtattagaa gtgacagcat gtagcaggtg ggaagtgggt actacattgg ctgttatcgc
     8101 ggaacgggct ggcccaccca ttgtacttgt aactccgagt attgcgacca ttgcatatat
     8161 gtgccaaggc tttaaagaat cggtgattaa tagaattgtg gcgagggcta ttaagaagaa
     8221 gctaaagcac tgagtgatcg ctattagctt ttttcgatca aactggtcag cgaatgctcc
     8281 tccaaataat gaggccggca tttggacagc aagttgaacc aatcctagta atccgagttg
     8341 tattcctgaa cctgtctcct cataaagcca cacaccaaat ccaagcagcc ggatctgcat
     8401 ggtaactgag gcacacaacc ccgataccca tagcattctg aaatttggaa acctgaaagc
     8461 agaccaaggt tttatattgg gagagtcttc tgtattacgc aaaaagtata aaacctactg
     8521 gcgcatttgc ttcagggggt tgactctgat ttctatatgg tttcacggat agttattata
     8581 aactaagtga taattcaaag gtttaaaaag cgggggctat aagattctgt ttatacattt
     8641 ggtatcgtct tgcaagcaat tcgtcggagt gatggtggca cgattgtggg tgttgtttcc
     8701 catttgtatt gggttgtcgc tagcaatagt aggggttgag ggattatacg aaccggggtc
     8761 tataagcttc ggcatttatg attggggacc tcgattagtt gggtctaacg attgggtgta
     8821 ttgttccttt attccttcag gactactgat cacgggatat tttttggttg gcatgtctgc
     8881 tttgaaggac aatcctctgg ctcgcatctt taaaaaggtc gttctttaca tttttggatt
     8941 tttatttctg gggattggaa tagcaggata ttttctgccg atgttgcccg gcactatatt
     9001 tcttattctt tctgccgttt gtttcatgcg gtccaatgaa aagatgtatc gctgggtcac
     9061 ggagcataga ctttttggaa gacatgtaaa acaatttatg gaaacgggcg ctatgcctaa
     9121 aagggccaaa ttcatctcta ttgggtgcat atggtttttc tcagccatat caacatcgcc
     9181 gataacccct tacggctggc aattcaaagc tatagtggtt tcccttgctg ttataggtac
     9241 gttctatata ctgacgcgtc caaactccaa atagagatta tttactagag aaccgaaact
     9301 ttgatttgtg ggttagcttc tcccctctta agtgcttcta ttatggtatc tgcaagtttt
     9361 ttagctgatt gaggacttaa ctcgacagca actctcgatc caaccccccg cgattggtta
     9421 acaaaatcta tgttaacgcc gtactcccaa ggtgcgttga atggatgatc ataagatacg
     9481 ttgaccattt ctagggggaa ccatccatcc ctgcctttcc cacttccctc gacctctgtt
     9541 cgttcacata tcattgtgca cataatgaat atttcctttt aaatttaact caaagttcta
     9601 cttaaaaacg ttagcatttt gtcccatcca tcaaccgctg aactctggtg atatatgggc
     9661 cggtcgaaat aaaagaagcc atgaccagct tctggatacc tgtgaaactc tatatccttt
     9721 cctgccgctt tcaactctgc ttcgtgttgg tttacttgtt cgggagttgg gctttgatct
     9781 aaatccccaa aaatgccca
//
LOCUS       DASCNS010000188         9881 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001514104, whole genome shotgun sequence.
ACCESSION   DASCNS010000188 DASCNS010000000
VERSION     DASCNS010000188.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001514104"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="QF838_06740"
     CDS             <1..1024
                     /locus_tag="QF838_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HJO60412.1"
                     /translation="YLAVSIELCKVESFLCYPHSRFIQILTHSEENVETLEKRKPAGL
                     LPTVFQIFLSLLWGGNHVSIKASLEYGTPLQVGWMRFVVGGTVTFAYMLLRKESFTVN
                     KNEIRPLLIVGALFTVQIMFMNVGQYLTTAGHATALNSTYPIWAAVIAHFLVPTDTLT
                     RWKVLAIVLSYIGILTIVFGDVGIVVPGVSIEGDLMSLLSAILLGLRLVLMSNFAQNM
                     SEAKIMFGQLVIGITLFFIGSLLFESPQYTIESRFWLALAYQGFVIAGFGFLANAWLV
                     KKYLPSTVTFYSFIQPPAGVILAWLVLSEDPGRGLLGGLVFVVAGAITFGSESFVKAK
                     KREALV"
     gene            1024..2142
                     /locus_tag="QF838_06745"
     CDS             1024..2142
                     /locus_tag="QF838_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60413.1"
                     /translation="MKIVDVISEVFEWERPGIWNGGHFYGPGRLHKVTVKTDEGIEGF
                     GWNGGTAAERPLNVFPPFVDYFRDLLIGRDPFETRKIAEDLGEKHIKILGPGGVNTQV
                     LAAINIACWDIKGKALEKSVHELLGGSQDRIRTYIAGGYYAEGKGLKELQDEVLFNIN
                     EMNAGAVKIKIGDPNEGIKGDMLRVEAARKAVGDDVVLMVDANCALDLNQSLAFAHEL
                     DKFGVYWFEEPMPIHRYKEHGVLKENSNVKIATGENGYHISHFETLLDHQGADILNVD
                     VAICPGYDIAKDITDLAKEKGVSIAPHGCQELQLPLAAGIPHGEFLEYYPVAVDPLRA
                     EMFIPRMVPDTDGYVTVPDRPGIGFELNMELLNRYKLS"
     gene            2139..2681
                     /locus_tag="QF838_06750"
     CDS             2139..2681
                     /locus_tag="QF838_06750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO60414.1"
                     /translation="MIDFPMIVHYLEIFSKENINRKLIVQSDLKITKVREPDPAFAQW
                     LYRTVGREWYWVDRYYWSLNEWEEWISDSCISLWIMELSDRPIGFYMLDPQDNNNVEV
                     SYFGLLPGYLGSGLGGHLLTNALDDAFSMGASRVWLHTCSLDHPYALSNYEARGMNVY
                     KVEDDLQHIPDGWPVPDDLT"
     gene            complement(2686..3639)
                     /locus_tag="QF838_06755"
     CDS             complement(2686..3639)
                     /locus_tag="QF838_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO60415.1"
                     /translation="MSVTVGTGNFKYEAVDSWPILPGGATLIETPGVAVDSQDEIYTF
                     SRNTDHPVMVFNRDGNFLRGFGAGIFSNRTHGILIGPDDTVYCADDGIHTITKFTREG
                     ELLMTIGTPGKSSEIWKGEPFNRPTHAAVSKKSGDIFITDGYGNFRVHKYSAEGNYIK
                     SWGEPGIEPGQFLRPHNIAVDDNDRVIVADREAHRVQIFDTDGNVIDVWNNIFMPNGL
                     TIGPDGNIYIGELPGMTQADPTPPNHGHNISIISPSGEKLGRIGHPEEGEEPGKFIAP
                     HGIGVDSHGDIYVGEVSYTIRGSHMNPPKELRSLSKLRKVT"
     gene            3857..5290
                     /locus_tag="QF838_06760"
     CDS             3857..5290
                     /locus_tag="QF838_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60416.1"
                     /translation="MTHFGVKSEINKNPELSNNEVILYSRESTKLPVVEDFSKTIKNI
                     SKGIPLSSALSRYPDSVKFEFDSKNYEQIFASVISNIARITPKIERSNLGVIYINMHG
                     LSEIYGGEAKLVTHILDSVPYFLEPRFGISVNKFSAYAAAFSSTPGGSTKILKNIDSF
                     LATFNVDILPIKRSSIINFHKFGMHTIGDIAAQDYGSIYSKFGDEGCRALSLSRVENS
                     DYISSNKPVQDITEQLSLPFPSDSLSVLFTALEFLIQKAFIRPVLRSKYIRKISILLE
                     LIGSPVWTKSLILKRPVGNSNDLCLLLRSELENLELPGLIEDISITITDFVGEHGIQY
                     RAFKEIRDHLDERKDQLIKVDRHIRKKIPAKNSLHKLLTVNPEHPVPERRALQVSIDP
                     DTKFSVMSINLPQKITVQELNNIPKFLSLSNGYNLKVKVLDIWKIDLWWISDPISRTY
                     YSISTPDHKIMTIFKDSVKKRWYRQNY"
     gene            5269..8439
                     /locus_tag="QF838_06765"
     CDS             5269..8439
                     /locus_tag="QF838_06765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HJO60417.1"
                     /translation="MVSSKLLMHVSSKYVELHAHSFYSFGEGASHIDELLSRAYELAY
                     PAMALTDYNMCGALEFSRQSDHFGIKPITGAEIILEDNSHIVLLAKNRIGYSNISRLL
                     TLANGSDRRDPKLDPMHIPEYASGTILLTGAQNGLISNAIKEHKIDRAKAVLNKYREY
                     FEDDSIYIELNRNFMYGDSSRINSLISIGSDMKLPLVATNNVHYHIADRYKLQNVLTS
                     IRSNTNLDKLTHLLKINSEFYLKSESQMQRLFKSIPKAITNTLAISDMCEFNLSSDLG
                     YELPSPSVPDGFTPFTYLSRLCYEAAQRRYGAVSNSVDNRLREELRLIKKHNLAGFML
                     LYREIVLIAREISVDMGKLDSEEPIESRSPGRGRGSSVAMLTGYLIGISHVDPLLYNL
                     TLERFVPEDLKTLPDIDIDFPRSIREELIPRIHEYFGPQFAVLTGMITRYKLKGILKD
                     LGKVFGISDVDIRNLSKKIHNSDPISLKDEMLSIPEFKNAVDLPEWQNIISLAPQLKD
                     APKTLGQHVGGMILSSSPISDMVPFRKSAIEGRYIIDWDKDSIADAGFAKIDILSLPV
                     LDQIDESISLIQNTTGKLVDITQVTPEDNEVFDMINTGLSKGVFLLQSPAQLKMGQRL
                     LSRSLNDLAYQVALIRPGVGVQGNSVSQFVERYRHGTPWQYDHHLEQRALERTCGIIV
                     WQEQVVQLISDISGISQAESDEMRRGFAKTNNESLIKGYWHKFVKGAIKKGVSEEIAH
                     KIFSKINGQYMFPESHSHAFAVSAYQSAWLKTHYPLEFYVSLMNNQPMGFYPLETIKQ
                     DARKFGVDFYNPDINHSQNKCTVNGNSLLIGLEFVKNIGIQNSQNIIDERNVRGPFNS
                     VGDFIRRVQIKPDSAESLIFSGAFDSLISNRRLALWESGLYKGPSGSQITLPISMDDN
                     IPGLADFSSYEKMILEYKAIDMYPKGHIMDFIRPLLPSNVSSTYDAYSMREGESVSVC
                     GMGIARQHPRGYKGAIFVTVEDESSDIQLILWPDIHKKYMRVLEDPIILATGKISRQD
                     GTTSVVVSYLETLEPKIGLPSAHNWY"
     gene            complement(8459..9112)
                     /locus_tag="QF838_06770"
     CDS             complement(8459..9112)
                     /locus_tag="QF838_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60418.1"
                     /translation="MSTKKLTVIEMTNSLAQATYELSSESLQNMTLDLDQLPNELTEF
                     SLLRESILDNRTMATHGFPGNTEERYDEAGRLTGYLREFASPSAVPQSKDGSDIVAAT
                     VVHLFKDQEYSEKWMTDTFVAQFRNNVGNEVGENQILVAVEELEIDDFYDRSIGIRAV
                     QSGEEGILSTTVIDFKIGRLLGVSFVVTIGDHGRTELTTMLGLELERNIVRELLATS"
     gene            9220..>9881
                     /locus_tag="QF838_06775"
     CDS             9220..>9881
                     /locus_tag="QF838_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024810.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HJO60419.1"
                     /translation="MLGQSKLRYLLLTTILYLLLAILGCDEFTKHDEYDFVQTSNLIS
                     SPGSPVAPGSIFNSRINHTATFVASGKILVVGGESDNGGISSLEIYDTENGSWVNGQP
                     LPNPRFGHTANLIGRESLLIAGGEDGENITPSTVVYNANTDTWTSSLDMLQPRTQHAS
                     SNLLDGRVIVVGGANTSGLLSSAEIYDPVLQHWTSSGESLLPRIGHSVTTLKNGMILV
                     VGG"
BASE COUNT         2938 a         1759 c         2049 g         3135 t
ORIGIN      
        1 ctacctagct gtctccattg agctatgtaa ggtagaatct tttctatgtt atccccacag
       61 taggttcata caaattttga cccattcgga agaaaatgta gagacacttg aaaaaagaaa
      121 gccggcgggg ctgctaccca ctgtttttca gatattcctc tctttgcttt ggggggggaa
      181 tcacgtaagt ataaaagcat ctcttgagta tggaacccct ttacaagttg gatggatgag
      241 atttgtggtc ggagggacgg taacttttgc ttatatgctc cttagaaaag agtctttcac
      301 ggttaacaaa aatgaaattc gcccgctttt aatagtagga gcccttttca ccgtacagat
      361 tatgtttatg aacgtcggtc aatatttgac cactgccgga catgccactg cgttaaattc
      421 cacttaccct atttgggcgg cagtaatagc ccatttcttg gttccaaccg atactcttac
      481 tcgatggaaa gtattggcaa ttgttttgtc ctacataggt atattgacga tagtctttgg
      541 ggatgtcgga attgtcgtac caggcgtttc catagaaggc gacttgatga gtcttttgtc
      601 agctattttg ttggggttgc ggttggtttt gatgtccaat tttgcccaaa atatgtctga
      661 ggcgaaaatt atgtttgggc aacttgtcat tggaataacg ttatttttca taggtagttt
      721 gctttttgaa tcccctcaat acaccatcga atcaagattt tggttagcac ttgcctatca
      781 aggatttgtg atcgcaggat ttggatttct tgccaatgca tggttggtaa agaaatattt
      841 accttcgaca gtaacatttt actcatttat acaaccacct gccggtgtta tattggcttg
      901 gttggttctt tcagaagatc cgggccgagg actgctaggg ggtcttgtat ttgtagttgc
      961 tggtgctatt acgtttggct ctgagtcgtt cgttaaagcc aagaaacgag aggcattagt
     1021 ctaatgaaaa ttgttgacgt aataagtgaa gtttttgagt gggaaaggcc tggaatttgg
     1081 aatgggggac acttttacgg cccaggtcgg cttcataaag tcacagttaa gaccgatgag
     1141 ggaatcgaag ggtttggttg gaatggtggt actgctgctg aaagaccttt gaatgtgttt
     1201 cctccttttg tggattattt ccgtgacttg ttgattggaa gagatccgtt tgaaactagg
     1261 aagatagcag aagatttggg tgaaaaacac ataaaaatat tgggcccagg gggagttaac
     1321 acgcaagttt tagcagcaat aaatatcgcc tgttgggata taaaaggtaa agccttagaa
     1381 aaatcagtac atgaattgct aggagggagc caagatcgta tcagaacgta tattgctgga
     1441 ggttattatg ctgaagggaa aggacttaaa gaacttcaag acgaggttct tttcaatatt
     1501 aatgaaatga atgctggtgc tgtgaagatt aaaatcggcg atccaaatga aggtattaag
     1561 ggtgatatgc tgagggtgga ggcggctagg aaggctgtag gggatgatgt ggttttgatg
     1621 gttgatgcaa attgtgcgtt agatctgaac cagtccttag catttgctca tgaactagat
     1681 aaatttggag tgtattggtt tgaggaacct atgccaatac atcggtataa agaacatggt
     1741 gttttgaaag aaaactcgaa tgtgaagatt gctactggtg aaaatggata ccatatttct
     1801 cattttgaaa cccttcttga ccatcaaggg gctgatattt tgaacgttga cgtagcaatt
     1861 tgccctggat atgacattgc taaagatata accgatctag ctaaagaaaa aggcgtatcg
     1921 atagctccac atggctgtca ggaacttcaa ttacctcttg ctgctggtat accacatggg
     1981 gaatttttgg aatactatcc ggttgcagtt gacccactta gggcagagat gtttatacca
     2041 cgaatggttc ctgacactga tggatatgtt actgtgcctg acaggcccgg tattggattc
     2101 gaattaaata tggagctgct aaacagatat aaattgtcat gatagatttt cctatgatcg
     2161 tgcattatct ggaaatattt tcaaaagaaa atatcaatcg aaagttgatt gttcagagcg
     2221 atttaaaaat aacgaaggtt agagaaccag atcctgcttt tgcccaatgg ctctatagga
     2281 cagttggtag ggaatggtac tgggttgata ggtattactg gagtttaaat gagtgggagg
     2341 agtggatttc agattcatgt atatctttgt ggattatgga actttcagat aggcccattg
     2401 ggttttatat gttggatccc caagataata acaatgtgga agtatcttac ttcggattac
     2461 ttccaggata ccttggaagc ggattgggtg gtcacctttt gacaaatgct ttggacgatg
     2521 cattttccat gggggcatct cgggtatggc tgcatacttg tagtctagat catccatacg
     2581 ctttatccaa ttacgaggca cgtggaatga atgtgtacaa ggtagaagat gacttgcaac
     2641 atatacctga tggatggcct gtacctgacg acctgactta aaaggctaag ttacttttct
     2701 aagtttactt aggctacgaa gttcttttgg aggattcata tggctgcctc tgatggtata
     2761 tgaaacttca ccaacatata tatctccgtg tgaatctacg ccgataccat gaggtgctat
     2821 gaatttaccc ggctcttcgc cttcttcagg gtgcccaatt cttccgagtt tttcaccgga
     2881 aggacttatg atacttatgt tgtggccatg gttaggagga gtaggatctg cttgtgtcat
     2941 acctggtagc tcgccaatat atatatttcc gtctgggcca atagtcagcc cgttgggcat
     3001 gaatatattg ttccaaacat ctataacatt gccatctgtg tcaaaaattt gtactcgatg
     3061 ggcttcacga tctgcaacta tgaccctgtc gttgtcgtcc acagctatat tgtgaggcct
     3121 gaggaattgg ccaggttcta tacctggttc tccccatgat ttgatgtaat taccttcagc
     3181 tgaatattta tgaactcgaa aatttccata tccgtcggtt atgaatatat ccccactttt
     3241 ttttgatacc gcagcatgtg ttggacggtt gaaaggttca cccttccata tttcggagga
     3301 tttacctgga gtgcctattg tcatgagtag ttcaccttct ctagtgaact tagttattgt
     3361 gtggattcca tcatccgcgc agtagacagt atcatcaggc cctatcaaaa ttccgtgtgt
     3421 tctattgctg aatatacctg ccccaaatcc ccttaagaag ttgccatctc tattaaaaac
     3481 cattactgga tggtcagtat tgcggctgaa tgtgtatatt tcatcttgtg agtctactgc
     3541 cactccgggt gtttctatca gtgtggctcc accaggtagt attggccatg aatctacggc
     3601 ttcatattta aaattgccag tgccgactgt aacgctcatt ttgcccctcg ttattgtgaa
     3661 aatgtctttg tctaccttga acataacatt agctagaaac acagtcaatt ttgttgaaat
     3721 acacaaaagc atcctattat cttagtattg aaaatagaac ataagttcta ttataattat
     3781 acccctagct ttatcaacat ataaaaaaca atttaaaaat atgcctaacg aaaattctaa
     3841 aatcggatgt cttttaatga ctcatttcgg agttaagtca gaaatcaata aaaatcctga
     3901 actctctaat aatgaggtta ttttgtattc tcgcgaatct actaaattac ctgtagttga
     3961 agatttttct aaaactataa agaatatttc aaaaggaatt ccactttcgt ctgcattgtc
     4021 cagatatcct gattccgtaa aatttgaatt tgatagtaaa aattacgaac aaatcttcgc
     4081 ttcagttata tcaaatattg caagaataac tccaaaaatt gagagatcaa atttgggggt
     4141 tatttatata aatatgcacg gattgagtga aatatatgga ggtgaagcta aattagttac
     4201 tcatatattg gattcagtcc catattttct agaaccaaga tttgggatat ctgtaaataa
     4261 attttctgca tatgccgcag ccttttcttc tacccccggt gggtctacaa aaatattaaa
     4321 aaatattgat tctttcttag ctacttttaa tgtggatata ttgcctatta aaagaagtag
     4381 cattattaat ttccataaat ttggtatgca tacaattggt gatatagcag ctcaagatta
     4441 tgggtcgatt tattctaagt ttggagatga aggctgtaga gccttatcgt tgtctcgtgt
     4501 tgaaaatagt gattacattt cttctaataa acctgttcaa gatataaccg aacagctatc
     4561 tttaccattt ccttctgatt ctctttccgt tctttttaca gcgttagaat ttttaataca
     4621 gaaagctttt attcgacctg ttttaagaag taaatatatt agaaaaatat ccatcttgct
     4681 tgaacttatt ggatctccgg tttggaccaa aagtttgatc ttaaagaggc cagttggtaa
     4741 ttccaatgac ttatgtttat tgcttagatc agagctagaa aatttggaat tgcccggatt
     4801 aattgaagat atctcgatta caattacaga ttttgttgga gagcatggta ttcaatatcg
     4861 tgccttcaaa gaaattcgtg atcaccttga tgaaagaaaa gatcagttaa ttaaagtaga
     4921 ccgtcatatc cgcaagaaga tacctgccaa gaattctctt cacaagcttc ttactgtgaa
     4981 tccagagcat ccggttcctg agcgaagggc actacaggtt tctatagatc ctgatacgaa
     5041 attttctgtt atgtctatta atctacctca aaaaatcacg gttcaagaac taaacaatat
     5101 tcctaaattt ctatctctat ctaatggata taatctaaaa gttaaagttt tagacatctg
     5161 gaagattgac ttatggtgga tctcggatcc gatcagtcgt acttattata gtatcagtac
     5221 acctgaccat aagattatga cgatatttaa agatagtgtt aaaaaacgtt ggtatcgtca
     5281 aaactactga tgcatgtcag ctctaaatat gtagaactgc atgcgcatag tttttattct
     5341 tttggtgaag gggcttccca tatagatgaa cttctttcta gagcttatga actagcctat
     5401 cctgcgatgg ctcttacgga ttacaacatg tgtggggctc tagaattttc taggcaatct
     5461 gatcatttcg ggataaaacc tataacaggt gctgaaataa ttcttgagga taattctcac
     5521 atagttttat tagctaaaaa caggatcgga tactccaaca tatcgcgatt actcacttta
     5581 gctaacgggt cggatcgtag ggaccctaag ttagacccta tgcatatacc tgaatatgcc
     5641 tctgggacca tactactcac tggcgcacag aatggcctaa tctcaaacgc tattaaagaa
     5701 cacaaaatag atagagcaaa agctgtactt aataagtaca gggagtattt tgaagatgat
     5761 tctatctata tagagttaaa tcgtaatttt atgtatggcg attctagtcg tataaattcg
     5821 ttaatttcta taggatcaga tatgaaactt ccgttagttg caacgaataa tgttcattac
     5881 catattgctg atagatacaa gttacaaaat gtgctcactt caattagaag taatacgaat
     5941 ttagataaat taactcacct tcttaagata aattccgagt tttatctaaa atcagaatct
     6001 cagatgcagc ggttgtttaa atctatcccc aaggccatta caaatactct cgctatatct
     6061 gatatgtgtg agtttaattt atcttctgat cttggttatg aactaccatc acccagtgtt
     6121 ccagatggat tcactccctt tacgtatctt agtcgtcttt gttatgaagc tgcgcagaga
     6181 cgctacggtg cggtgagtaa ttctgtagac aatagattga gagaagagtt gagacttata
     6241 aaaaaacata acttggcagg cttcatgctt ttataccgtg aaattgttct tatagcacga
     6301 gaaataagcg ttgatatggg taagttggat tcagaggaac ctatagaaag caggtcacct
     6361 ggaagaggta gaggatcttc agttgccatg ttaaccgggt atttaatagg tatatctcat
     6421 gttgaccctt tactgtacaa cttaacatta gaaagatttg taccagagga tttaaaaaca
     6481 ttacccgaca tcgatataga ttttcctagg tcaataagag aagaattaat acctagaatc
     6541 catgagtatt ttggacctca atttgcagta ctcacaggta tgataaccag gtataagtta
     6601 aaaggaattt tgaaagattt aggtaaagtt tttggaatat cggatgtaga tattagaaat
     6661 ctttcaaaaa aaatccataa ttcagaccct atctccttga aagatgagat gctgtctatt
     6721 ccagaattta aaaatgccgt tgatttaccg gaatggcaaa atataatttc tcttgcccct
     6781 cagcttaaag acgctcccaa gactttgggt cagcatgtag ggggaatgat tctaagctcc
     6841 tctcccattt cagatatggt cccctttaga aaaagcgcaa ttgaaggtag atacatcatt
     6901 gattgggata aagatagtat tgctgatgca gggtttgcaa aaatagatat actctctctt
     6961 cctgttttgg atcaaataga tgaatctata tctttaattc aaaacacaac aggtaagtta
     7021 gttgatatta ctcaagtaac tccagaagat aatgaggtct ttgacatgat aaacacaggt
     7081 ttatcaaaag gcgtttttct tctccaatcg cctgcacagt taaagatggg ccaacgtctt
     7141 ctatcaagaa gtttgaatga ccttgcctat caagttgctt taataaggcc tggggtaggt
     7201 gtgcagggta attcagtttc acagtttgtg gagcgatata gacatggaac accgtggcaa
     7261 tatgatcatc atttagaaca acgtgccctt gagcggactt gtgggattat tgtgtggcaa
     7321 gaacaggtag tacagctaat atcagatata tcaggaataa gtcaggctga atcagatgag
     7381 atgcggagag gatttgctaa aactaataat gagagtctta ttaaaggtta ctggcataaa
     7441 tttgtaaaag gtgcgattaa aaaaggggtt tcagaagaaa tagctcacaa aatattttct
     7501 aaaataaacg ggcaatatat gtttccggag tctcattctc atgcttttgc tgtttcagca
     7561 tatcagtcgg cgtggttaaa aactcattat ccattagaat tctatgtcag tcttatgaat
     7621 aatcagccca tgggatttta tcctttggag accatcaaac aagatgcgag gaaatttgga
     7681 gtcgattttt ataatcctga tattaatcat agccaaaaca aatgtacggt taatgggaat
     7741 tcacttctaa ttgggctgga atttgttaaa aatattggca tacaaaattc ccaaaatatt
     7801 atagatgagc gtaatgttcg aggccctttt aattctgtgg gcgattttat tcgcagggtt
     7861 caaataaaac cagattcagc tgaatcatta attttttctg gggcatttga ttcccttata
     7921 tctaatcgta gattggcttt atgggaatca gggctttata aaggtccgtc tgggtcgcaa
     7981 ataaccttac cgatttcgat ggacgataat atacctggtc tagctgactt ttccagctat
     8041 gaaaaaatga tattggaata taaggctata gatatgtacc cgaaaggcca tattatggat
     8101 tttattagac ctcttctacc ttcgaacgtt tcgagtactt atgatgccta ttcaatgaga
     8161 gagggtgaat cagtatctgt gtgtgggatg ggaattgcta ggcaacaccc aagggggtac
     8221 aagggagcca tatttgttac cgtagaagac gaaagttcag atattcaatt aatactttgg
     8281 ccagacatac acaagaagta tatgagagtt ttagaagacc ctattatttt ggctacagga
     8341 aaaatatcac gacaggatgg tactactagt gtggtcgtat cttatttaga aactttagaa
     8401 cctaaaatcg ggttaccttc tgcacataat tggtattgat ttccagttat atatttccct
     8461 agctggtggc tagaagttct ctcacaatat ttctttcaag ttctaggcca agcatggtag
     8521 tgagctctgt tctaccatgg tcaccaatgg ttactacaaa ggaaacccct aaaagtcgcc
     8581 caattttaaa atcgataaca gttgtggata aaattccttc ctcaccagat tgaactgctc
     8641 ttatcccaat ggatctatca taaaagtcat cgatttcgag ttcttccaca gcaaccaaaa
     8701 tttggttttc tccaacttca ttacctacat tatttctaaa ttgtgcaaca aaagtatctg
     8761 tcatccattt ttcagagtat tcttggtcct tgaaaaggtg gacaactgta gcggccacaa
     8821 tatctgaacc gtccttactt tgtggtacgg cagacggtga tgcaaattcc cttaagtacc
     8881 cagtaaggcg acctgcctca tcatatcttt cctctgtatt accagggaat ccatgagtag
     8941 ccatggtcct attatccaaa atactctctc tgagtagtga gaattcagtc agctcattcg
     9001 ggagttgatc gagatccaag gtcatatttt ggagtgattc acttgaaagt tcgtatgttg
     9061 cttgtgctaa tgaattcgtc atttctatta cagttaactt tttagtcgac atcaatatat
     9121 accctaagta taaaccggat gtccagatca gagttaactg ctaagcagat gttaaaattc
     9181 tcgctaagtg ttttgaacat gcacttggag tccatcgtta tgctaggtca gtcaaaactt
     9241 cgatacctgc ttttaactac aattttatat ctcttactcg ccattctcgg gtgtgatgaa
     9301 tttacaaaac atgatgaata tgattttgtg cagacctcga atctaatctc tagtccaggt
     9361 tctcccgttg ctcccggaag tatattcaac tcacgaatta atcacacagc cacttttgta
     9421 gctagtggaa aaattttggt agtaggaggt gaatcggata acggaggaat cagttcactt
     9481 gaaatatatg acaccgaaaa tggtagttgg gttaatggac agccattgcc aaatcccagg
     9541 tttgggcata cagctaatct aattggtagg gagtcgttat taatcgcagg gggggaggac
     9601 ggagaaaata taacgccatc tacggttgta tacaatgcaa atactgacac gtggacttct
     9661 tctttggata tgctacaacc aagaacacaa catgcgtcct ccaatctcct agatgggaga
     9721 gtaattgtag ttgggggggc taatacttca gggttacttt cttctgcaga aatatatgac
     9781 ccagttcttc agcattggac atcttcagga gaatcccttt taccgagaat cggccattct
     9841 gtcaccacat tgaaaaatgg aatgatatta gtggttggtg g
//
LOCUS       DASCNS010000189         2054 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001516129, whole genome shotgun sequence.
ACCESSION   DASCNS010000189 DASCNS010000000
VERSION     DASCNS010000189.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001516129"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(329..979)
                     /locus_tag="QF838_06780"
     CDS             complement(329..979)
                     /locus_tag="QF838_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013691.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HJO60420.1"
                     /translation="MSKDERFLPAYSPNFGWFYQNLDDCEYWSCEKPANCIVFENDTP
                     SKIHLRVTQGDGHMMNLCHRHAGDAEKEEVNRCTTGHCFNIKQSKYEKCFECSANKSK
                     AKYTRYIHEASPIREAQDEGLFHVYVLGLSDKTFYVGHTSDLRMRMGEHKSGKAMDTA
                     GKQPQLVWFAETSTRKSASTLEAEIKKLRDKNDRKLRRMVIDFSDLVKETYWYKHS"
     gene            1253..1699
                     /locus_tag="QF838_06785"
     CDS             1253..1699
                     /locus_tag="QF838_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60421.1"
                     /translation="MPEQHPSMFDVQSVDLSVIKDILVRTNSLEALKELEKEINQLEL
                     IDIDEAYKLAQYLYRESQSEKLPAKATIQGWISEGRLHQRGYLHTKRNVGRPKVLISK
                     IDLLELLNNPPKVGRPVGSDTGVNSPTGNVVNKKTPEAKTMNLFDL"
BASE COUNT          572 a          413 c          437 g          632 t
ORIGIN      
        1 ctaaagggcc tttaccctca accgctaccc ttagtttgat accgttgctt tcaattgttt
       61 taagggcaga tttgttatcc aatgtcaaca ccaccttaag caagatttga atcagtagcc
      121 cgaattcaat catagctgaa actgatggta gttagtatta attggcagct agatataaag
      181 acgcgttacc tactgttaaa gactagggat caaagtttaa atcatggccc ctgggtgggg
      241 tggtctaggg tacgatcttc tcaaggctgg gatcgtcagt ttgaatctgg tcggggtcgc
      301 cactttctcg gcttttttat tgttccactc agctatgttt gtaccagtat gtttctttaa
      361 caaggtcact gaaatcaata accattcttc taagctttcg atcattttta tctcgtagct
      421 ttttgatttc agcctcaagt gtagaagcgg attttctcgt cgacgtctcg gcaaaccaca
      481 caagttgtgg ttgttttcct gcggtgtcca tcgctttgcc tgatttgtgt tcacccattc
      541 tcatccgaag atcactggta tgacctacat aaaaagtttt gtctgaaagg cccaacacat
      601 aaacgtgaaa taatccttcg tcctgagcct cccttattgg agaggcttca tgtatgtacc
      661 gggtgtattt tgccttggat ttattagcgc tacattcaaa acatttttcg tattttgatt
      721 gtttgatatt aaaacagtgc ccagtggtac atctgttgac ctcttctttt tcagcgtcgc
      781 cggcatgtcg atggcacaga ttcatcatgt ggccatctcc ctgtgtgacc cggagatgaa
      841 tttttgatgg agtatcgttt tcaaaaacta tacagttggc tggcttctca caactccagt
      901 attcacaatc atccagattt tggtaaaacc agccgaagtt aggagaatag gctggcaaaa
      961 acctttcatc tttcgacatt ggattggaga aagtcactat atatcctcgc tctattttcg
     1021 gtatgccagt tcagaatacg atcagatcga agatattaat agcgtaatag cacctgaagt
     1081 gatctaggtg aaactttgca agggtttggg attacgatgc tctggcagat gaccgtccaa
     1141 gaagctatgt ttcgttcttc acaaccagtt catgctggta attttcccaa agaatatata
     1201 ttttgcatgc agttggtgta gtatccactt acacggtttt ttgggggtta ttatgccgga
     1261 gcagcatcct agtatgtttg atgtccagag tgttgatttg tccgtgatta aagacattct
     1321 tgtccgcact aattcgttgg aagcactgaa agagttagaa aaagagatca atcaacttga
     1381 actgattgat attgatgagg cctataagtt agcccaatac ttataccgtg agtcacagtc
     1441 agagaaactt ccagcaaagg cgactattca aggttggatt agtgaagggc gtttacacca
     1501 gaggggatat ttacatacga agagaaatgt gggtaggcct aaagtactaa tctctaagat
     1561 tgatttgtta gagttactta acaacccgcc taaagtgggg aggccagtag gatcagatac
     1621 aggggttaat tcaccaacag gaaatgtggt gaataaaaaa actcccgaag caaaaaccat
     1681 gaacttgttt gatttgtaat tccccatttt ttatcaaagt agtgtttgga aaattttggg
     1741 tgagccttta actgtttcat tcatgggttt ttagccgaga cccgacctgg gaaagagtcg
     1801 ctcaacattg ataaatttcc tatttacacc gctctcttgc caagagctat aatccgcata
     1861 gatagagata catctcaata atcgtggagg aagacaatgg caggcataga agaaaatctt
     1921 gcgttcgcac cggcgacaga actcaaggaa cttatagcaa acaaaaaggt ttctccggta
     1981 gaaatcactc aactttacct ggatcgtata aatactctag atgggcagct caattcttat
     2041 cttactgtca catc
//
LOCUS       DASCNS010000190         7453 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001524152, whole genome shotgun sequence.
ACCESSION   DASCNS010000190 DASCNS010000000
VERSION     DASCNS010000190.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001524152"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="QF838_06790"
     CDS             <1..1016
                     /locus_tag="QF838_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HJO60422.1"
                     /translation="DAHIGTDIKHGRHSLSEPILQAEKGGDDGGPPFDWDICVNLGDF
                     TGSQVPPTDDEGPLVVEQYLSSKKHRREDFYDLIGNHDSSHFSERPSQWWFQKWIDPM
                     GRNTQFSHVDNSKRPFPVSGNWEHYSFDVGNITFLMIADRNDLPPPIGRGDRGGYPAG
                     AISEETFFWWKKKVGENRDRILVTCAHHMVKETTVATGLNEGCDGNYHGKFLDGAPQG
                     SSFIYFVGTQPDSGRIESFLEANEPALDMWLGAHTHTHPDDVVNGRSHIEQKYGVNFA
                     NISALTKYHGHTCMPMSRRLTFTENSDLVKIQCYLHTSDHAPQGWYSPVERTLKLRYK
                     FRW"
     gene            complement(1140..2309)
                     /locus_tag="QF838_06795"
     CDS             complement(1140..2309)
                     /locus_tag="QF838_06795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJO60423.1"
                     /translation="MTETCDAIIIGGGVMGASIISNLSSSGLTKTILLEKSTIGAGST
                     GRSSGAIRMHYSTKVNASLAHESLKVFANFSDLVGGDVGFVRTGYMVFAPSEASQVFR
                     ENISMQQDVGISTKEIDHKEAAELAPVFKISEGEHIAWEDDSGHADPSATALAYINHA
                     RANGATIHLETPVTDIKISESGPHQVITEKACFESENVIIATGPWTGEFLKKIDVSLP
                     LLPTRHEVFLIKRNLNKIPTHPGGGDMANLIYFRPEGHDLTLVGNGNHEETANPDSYN
                     QKYNLSYAADVWTRLTKRIPSIADAELFTGYSGLYTSTPDLHPIIDSVDGINGLFVCS
                     GFSGHGFKLAPAVGTVMAELVTQGKASTVDIEPLKMNRFADGKLNTTKYSFKVIA"
     gene            2539..3078
                     /locus_tag="QF838_06800"
     CDS             2539..3078
                     /locus_tag="QF838_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016840.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HJO60424.1"
                     /translation="MDLLEFATEMFEEYRSRLYTYLDGLTTEELNWRLDSSANSIAFI
                     VWHTARVEDRWFQVFCQGKADLWIEDGWFDKLGMDKNQPIAKYKPKELEDFPILTMGE
                     LKSYFEVVRAGTQKFLVNISTDELGKAPGRSPFPESSSASAFAKFTIQRMFRQLIQEE
                     MQHLGQVGILRGLQRGLDK"
     gene            complement(3599..4528)
                     /locus_tag="QF838_06805"
     CDS             complement(3599..4528)
                     /locus_tag="QF838_06805"
                     /inference="COORDINATES: protein motif:HMM:NF016852.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HJO60425.1"
                     /translation="MKDINEEGAAAYEKSVVEPKPMDPISAAERLIDIKRILDQLGAV
                     FFIASGTCLGAVRDGQFIEWDDEMDMGSVIGLNGLDEKTIDRGITAFEDNGYYVHVDR
                     SSRHFGVHVVKESIRADWTCHRIVGDSVFQYPGVRTPLALFTQLKEIDFIGEKFLVPN
                     PPEKYLETKYGSNWATPKGPGFEEEVVSRIQDGDILTSRQRLSKFITLRLAPWRAGRL
                     TVLDENDAPVSGAEVALIGVGRSKTNSKGHARLYIPEKYDYAISVKYDDHEELLYTEE
                     LMPRGTYVYRPGPDVSPEEHYKAGVRAMALSHE"
     gene            complement(4602..5327)
                     /locus_tag="QF838_06810"
     CDS             complement(4602..5327)
                     /locus_tag="QF838_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HJO60426.1"
                     /translation="MKGIIIAAGYGSRLKALTANTPKSLIEIEGKSIITYPIDALLAA
                     GISDISIVVGYRADVIINYIEKLYPSINFSYNKDYDGDNALSVFAARSFIGTDPFVLA
                     MGDHLISSSLVTTLLSDSTITRTLCVDSESKNASQVNDGTRVMADSKGRIIEIGKDLK
                     DWQSIDTGLFMMDSEVLDNIKHLMARQGKNVTISEVVQHMADNGRHFQTCDVSGQFWA
                     DVDTEEDRDSVSVLIKEGQGHDL"
     gene            5707..6591
                     /locus_tag="QF838_06815"
     CDS             5707..6591
                     /locus_tag="QF838_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013595.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO60427.1"
                     /translation="MVTDRFGFGDFQYEVVPDWGINSELGVVSDVATDSNDRVYLAVR
                     NTAHTETPSGVILVLDQHGNLLDSWGQDLFSTPHGIWISKDDEILLADSSDHTVRSYT
                     TDGRLEFTMGTQGKTGESGCPFNKPTRAALSNSGTLFVSDGYGQDRIHKFTGDGDWVM
                     SFGETGKGSGFLNLPHDVTVGDDDQVYVLDRENRRCQVFDQDGRYIAEFEGLRSPNDM
                     VIDESGFFHIAEGWYQEIASICVMSPEGTIVGRWGERGDGPGFFKGAPHGLWIDSKGD
                     MYIAEVAAEKAIHKFTRI"
     gene            6717..7235
                     /locus_tag="QF838_06820"
     CDS             6717..7235
                     /locus_tag="QF838_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60428.1"
                     /translation="MITKFDAFYGGHVEIDNFGFQGIPVDDRWLSDEHLSTALDIAKQ
                     FSILMDRNGFDTLWLSEHHFQREGYGCIPNIPMLSIYLSQFTQNLNFGGFFNVITAWH
                     PLRLVEDFAMADILTDGRVRLGVGRGYIAREVETLGGFLEDNDANRELFEEQIEVLRK
                     AWDNPSFSHHGK"
BASE COUNT         2011 a         1544 c         1717 g         2181 t
ORIGIN      
        1 ctgatgctca tatcggaact gatattaaac acggcaggca cagcttatca gagccgattt
       61 tacaggctga aaaaggggga gatgatggag ggccaccgtt cgattgggat atttgtgtaa
      121 atctaggtga ttttactggt tctcaggttc caccaactga tgatgaaggc ccactcgttg
      181 tagaacagta tctatcatca aaaaaacatc ggagggagga tttttacgat ctgatcggaa
      241 atcatgatag ttcccatttc tcagaaaggc catcacagtg gtggtttcaa aaatggatag
      301 atcctatggg aagaaacact caattctccc acgtagacaa ctcaaaaagg ccctttccgg
      361 tgtctggtaa ttgggaacat tattcttttg atgtcggtaa cataaccttc cttatgattg
      421 cggaccgtaa cgaccttcct cctccaatcg gcagaggtga tagaggtggg tatccagccg
      481 gtgcgattag tgaagagaca tttttttggt ggaaaaagaa ggttggagaa aatagggata
      541 ggatccttgt aacctgtgcc catcacatgg tcaaagagac cactgtcgcc acaggtttaa
      601 atgaagggtg cgacggaaat tatcatggga aattcttgga tggtgcccca caaggttcat
      661 cttttatcta ctttgtagga actcaacctg attcaggtcg aatcgaatct ttcttagagg
      721 caaatgaacc tgctctggac atgtggttag gggcacacac acacacgcat ccagatgatg
      781 tggtaaatgg tagaagtcat attgaacaaa aatatggagt taatttcgcc aacatttctg
      841 ctttgaccaa ataccacggc catacatgta tgcctatgag tcgacgttta acattcactg
      901 aaaatagcga tttggtaaaa attcaatgct acttacacac gagtgatcac gcccctcaag
      961 ggtggtattc tccagtagaa aggacactta agcttcgata taaatttaga tggtaggtct
     1021 taccggcaag gcttatttgc tataaaaata gcctgagaca ctagatgcca ctacaaatgg
     1081 gtcgctctat cctaaccggg ttggttaatt ccgaaatatg aaggatacaa tgacgttggc
     1141 tatgcaatga ctttaaagga atatttcgtt gtgttgagct ttccatctgc aaaccgattc
     1201 atttttaatg gttctatatc tacagtgctg gcttttcctt gagtcaccaa ctctgccatc
     1261 accgttccga ccgcaggagc aagtttgaaa ccatgtccac taaaaccgga acaaacaaac
     1321 agaccattta taccgtccac agaatcgata attgggtgta agtcaggggt gcttgtgtat
     1381 agaccggagt atccagtaaa tagctctgcg tctgcaatag aaggtatcct tttagtcaac
     1441 cgggtccata catcggcggc atacgacaga ttgtactttt ggttgtaaga atctgggttt
     1501 gcagtctcct catgatttcc attaccaacc agggtgagat cgtgaccttc tggtcgaaaa
     1561 tatatcaaat ttgccatatc tccccctcct gggtgagtcg gaattttatt aaggttcctc
     1621 tttattagaa aaacctcatg tctggttggc aaaagaggaa gagaaacatc tatttttttc
     1681 aaaaactccc ccgtccatgg cccggtggct atgattacgt tctctgattc aaaacaggct
     1741 ttttcagtga taacttggtg cggccctgat tcagatattt ttatatccgt gactggtgtt
     1801 tcgaggtgta tggtagctcc gtttgctctc gcatggttta tgtatgcaag tgcggttgca
     1861 gaggggtctg cgtgaccaga atcatcctcc catgcaatat gttctccctc ggatattttg
     1921 aaaaccgggg caagctcagc ggcttctttg tgatcgattt cttttgttga gattcctacg
     1981 tcttgttgca tagaaatatt ttcacgaaat acctgagatg cttctgaggg ggcaaaaacc
     2041 atatatccag ttcttacaaa accaacatct cctcctacta ggtcagaaaa attcgcaaac
     2101 actttcaggc tttcgtgagc caaggaggca ttcactttgg tggagtaatg catccttatt
     2161 gcgccagagg atctacccgt agaaccggct ccaattgtag atttttctag aaggatcgtc
     2221 ttggtgagtc cgctgctgga gagatttgaa attatgctcg ctcccatgac tccaccgcca
     2281 ataattattg cgtcacatgt ttccgtcata ttcaatcctc tatatgtggg atctggtagg
     2341 tgaagatata taataatcta tatgtgcagt ttctccgggt attatgaatt gtccacgaac
     2401 agtattcgcc ttatttactc aaccttcctc tggggaacta taatccccaa cttacagctg
     2461 ggcaaggagc ctcgtcaaag gtatatatca actcaggcgg gagtgaaaag acaaagaaat
     2521 ataaggagcg agggttcaat ggatctacta gagtttgcta ctgaaatgtt tgaggaatac
     2581 cgcagtcggc tgtatacata tttggatgga ctgaccactg aggagctcaa ctggcgtctg
     2641 gacagctcgg ctaattctat cgcctttatc gtatggcata cggcgagggt tgaagaccgc
     2701 tggtttcaag tgttctgtca aggcaaagcc gacctttgga tcgaggatgg ttggtttgac
     2761 aaacttggca tggacaaaaa tcagccgata gccaaatata agcccaagga actggaagat
     2821 ttcccaatat tgaccatggg tgaactcaag tcatactttg aggtcgtaag ggcaggaact
     2881 caaaaatttt tggtgaacat cagtaccgat gagctaggaa aggctcctgg taggtcgcct
     2941 tttcctgaat cctcgtcagc cagcgcattt gcgaaattca caatccaacg gatgttccgc
     3001 caattgattc aggaagaaat gcagcactta ggccaagtgg gaatcctacg aggtctccaa
     3061 cgagggttgg ataagtaaaa cagggtggca gatatatgta ttaattcaaa tgtttgatag
     3121 tgtggatgca ggttagtgga tctgatggta ggactgatat tagtcacaga taaaaaccca
     3181 cctagaactt aacctctatg acatagtcct tagcggtgat ggtggccagt tcgtcttttt
     3241 aatccgttac gtttactcga tttcttattg tcattaaggc gtttcacgaa ttccgctgtc
     3301 gtttcttctt gcttttccgg cttttttttg gatttgacca taatcgtccc tccattttgt
     3361 tgacaagatt gagtttactt accgctcgag gatccagtgg tacctgagct gtcttaggaa
     3421 tactcagagc catcgcatat tataatctgt tgtagataag cagccaaggt gttaaacatt
     3481 gttttttatc agtcatccat ccacttacac ggtaaaagca ggtcaacata tcactagaat
     3541 aaatttgtgt tgtgggtact gttcgtttga aattctaggc ttcaaagact gtattcccct
     3601 actcgtgaga tagggccatt gcccttacac ctgctttgta gtgctcctcg ggtgacacgt
     3661 cagggccagg ccggtagaca tatgttcctc tcggcataag ctcttctgtg taaagcagtt
     3721 cctcgtggtc atcatacttg acgctgatag cgtagtcata tttttcagga atatataatc
     3781 tcgcatgccc ttttgaattt gttttggagc gaccaacccc aataagggca acttcggctc
     3841 cactcacggg ggcatcattt tcatcaagga ctgttaagcg gcctgcacgc caaggggcca
     3901 atcgcagtgt aatgaattta cttagcctct gcctagaggt aagtatgtcc ccgtcctgga
     3961 ttctacttac aacttcttct tcaaaaccag gtcctttggg tgtagcccag tttgacccat
     4021 atttggtctc cagatatttt tccggtggat ttggtacaag gaatttttct cctatgaagt
     4081 cgatctcttt caactgtgtg aaaagtgcga ggggagttct tacgccgggg tattggaaca
     4141 cgctgtcacc gacgattcga tggcaagtcc agtctgccct gattgactct tttacgacgt
     4201 gaacaccaaa atggcgtgag ctacgatcga catgaacgta gtacccattg tcttcaaacg
     4261 ctgtaattcc cctatcgatg gttttttcgt ccaggccatt aaggcctatt acagacccca
     4321 tatccatttc gtcatcccac tcaatgaatt ggccatcacg gactgctccg agacaggtgc
     4381 ctgatgcgat gaagaatacg gccccaagtt gatctaaaat gcgctttata tctatcaatc
     4441 tttctgctgc tgagatcgga tccatcggtt tcggctctac gacagatttt tcgtaggctg
     4501 cagctccttc ttcattaata tctttcatgt gttctcccct tcatagacat aacttggtat
     4561 tgatgatttt tggtagcatc tgacggttgg gttgagttgg cttatagatc gtggccttga
     4621 ccttctttta taagaacgct gacagaatca cggtcctctt ccgtatcaac gtctgcccag
     4681 aactgaccgc tcacgtcaca tgtctgaaaa tgtcttccat tatctgccat atgttggact
     4741 acctcagata tggttacatt ttttccttgg cgggccatga ggtgtttgat gttatcgagc
     4801 acttctgaat ccatcataaa tagcccagta tctatagatt gccagtcttt gagatccttt
     4861 cctatttcta tgattcttcc tttagaatct gccatgaccc tggttccatc attgacttgg
     4921 gatgcatttt tggactctga atccacacaa agagtcctag ttatggttga gtcagataga
     4981 agggttgtaa caaggcttga gctaattaag tgatccccca tcgccaacac aaacggatcg
     5041 gtaccaatga aagaacgagc agcgaaaact gacaatgcat tgtcaccgtc gtaatccttg
     5101 ttataagaga aatttatact aggataaagt ttttctatgt aatttatgat cacatctgcc
     5161 ctgtaaccta ctactatcga gatgtcagaa attccagcgg ccaacaaagc atcaatcgga
     5221 taagtgataa tggatttccc ttctatttcg ataagtgatt ttggtgtatt agcagtgaga
     5281 gctttcagcc tactcccata acctgcagcg ataataattc ccttcatggc gtgttggctc
     5341 ctcaagttct cgtactttta aaaaaaacgg aatgtctgcg tggctcgtgt tgtggcctct
     5401 aactttcaag ggcacagaag cagactggtt cgtggtgaat catttcaacg ataagttata
     5461 gatccgaccg aagtgatgaa tagttgctcg gatacaaaaa acgctcaggt tgcgcgaggg
     5521 cagacaaagg cagaagggga ggcgttgcgg tatcgaaatt ccaccacatc caattagtca
     5581 tcttcacctg aattcacagc agtctcacca taacacagat tgtaaattcc cgccctcttc
     5641 ctttgatatt cgaaatggta ctatgcacgc attgaataca ggaagcatcc gtgaggtggc
     5701 cccgagatgg taactgatag gtttggattc ggagatttcc aatatgaagt tgttccagat
     5761 tggggaatta actcggagtt aggggttgta tctgatgtag caacggactc aaatgatcga
     5821 gtgtatttgg ctgttcgaaa cacagctcat actgaaacac cgagtggtgt catattggta
     5881 ttagatcagc atgggaatct gttggactca tggggtcaag atttgttttc gacacctcat
     5941 ggaatttgga taagcaaaga cgatgagatt ctacttgcag attctagcga tcacactgtc
     6001 cgttcctaca ctactgatgg tcgactagaa tttactatgg gaacccaggg gaaaacagga
     6061 gaatcaggtt gcccgttcaa caagcccacc cgtgccgctc tttccaactc cggtacgcta
     6121 tttgtatctg acggatatgg tcaagataga attcataaat tcacggggga cggtgattgg
     6181 gtcatgtctt tcggtgaaac aggtaaagga tcaggttttc tgaatttgcc tcatgatgta
     6241 accgttggag acgacgacca agtctatgtc ctggatagag aaaaccgtcg ttgccaggta
     6301 ttcgaccaag atggcagata tatagctgaa ttcgaaggac tacggtcgcc aaatgacatg
     6361 gttattgacg aatcaggatt ttttcacatt gctgagggtt ggtaccagga aatagcctcc
     6421 atctgtgtca tgtcaccaga gggtactatc gttggccgat ggggagaaag aggcgatggt
     6481 cctgggttct ttaagggagc acctcatgga ttatggatag attcaaaggg agatatgtat
     6541 attgctgaag tcgcggctga gaaagcaata cacaaattca ctaggatcta aatcatgtat
     6601 tcattaagta ttacctgtgt ggttggggta taaacgccta ttaaatttca ccgcgaagtg
     6661 tttaagaaag taaattgcaa atgttttgct tgattgaaaa ttaggagata tattttatga
     6721 ttacgaaatt tgacgcattt tatgggggcc atgttgaaat cgacaatttt ggatttcagg
     6781 gtatccccgt agatgaccga tggttatcag atgagcattt gtctactgct ttagatatcg
     6841 ccaaacaatt ctctatttta atggacagaa atgggtttga cactttgtgg ctttctgaac
     6901 accattttca aagagaggga tacgggtgca ttcccaatat cccaatgtta agcatctatt
     6961 tatctcaatt cactcagaat ctcaattttg gagggttctt taatgtgatc actgcctggc
     7021 acccattacg tctggttgag gattttgcaa tggcagatat actcactgat ggcagagttc
     7081 gtctgggagt aggaagggga tacatagcca gagaagttga aaccttggga ggatttctag
     7141 aggacaacga tgcgaatcgg gaattatttg aggaacaaat agaggtactg agaaaggctt
     7201 gggacaatcc ttcttttagt caccatggta agtgatacca aataccagcg gatgtgttgc
     7261 atcgtggtag caaactcgaa gatattactg tggttccaaa acctttacat ggggaagtgg
     7321 agatgtggca ttatttcttt gcgttttatt aggaggcatg ggatatgctc aaggaggtat
     7381 cctcgggaaa aaaatgagtg gttggagggt tatatgctgg tcattagtaa taagccttcc
     7441 tttggttata ccg
//
LOCUS       DASCNS010000191         7849 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001524461, whole genome shotgun sequence.
ACCESSION   DASCNS010000191 DASCNS010000000
VERSION     DASCNS010000191.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001524461"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="QF838_06825"
     CDS             <1..423
                     /locus_tag="QF838_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60429.1"
                     /translation="LDVSAYSLVSISRHASQLMTEGGNIITMSFMAAEKVFPGYNVMS
                     TAKAALENEVMQLANDLGPDGIRVNAISAGPLDTLSSRVISGYRDMKKAHLERAPMKR
                     NITHDEVASTALYLCSDMSSGVTGEVVHVDTGYHVMGI"
     gene            426..932
                     /locus_tag="QF838_06830"
     CDS             426..932
                     /locus_tag="QF838_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60430.1"
                     /translation="MPWAQTIVDALKEHEISLIAYVPDSSIHQVTKIVDKDEYFHLVS
                     ATREEEALGIAAGAYATGRNAAVFMQSSGFGNSINAVAGLAIPSRIPIPMFINLRGEH
                     GEFNIAQVTMGRSTRPILDVLGTPHYTLDSEERLKEKVKGALKLCYASREPLALCMTP
                     LLHGGKNV"
     gene            925..1497
                     /locus_tag="QF838_06835"
     CDS             925..1497
                     /locus_tag="QF838_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO60431.1"
                     /translation="MFRYSATKKIIGNLKREPVVSNLGPTSDELWHASDRDRNFYTYG
                     SMGLCSSIALGMALSCDEKVISLDGDGSILMNLGTIATIGREAPANLIVIVWDNEQWA
                     QTGHQKSHTAFGTDLELVAKSSGIEKSFTVADEEELDSILLQALNEDGPWFIVAKIED
                     EPYLSVAPVEPEHTLHRFRQTFVDRPDRVG"
     gene            complement(1461..2792)
                     /locus_tag="QF838_06840"
     CDS             complement(1461..2792)
                     /locus_tag="QF838_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60432.1"
                     /translation="MSNNSRINTKNIRNLHTFDSFRNSNYRLLWGTNISMYVSRWMQM
                     TTLSWFVLEQTRSPFSVGLVGFFGMVPFLVLGIFGGFLADKLNRKKLIVATQCLNLAA
                     ATVMSLLFIFGSVEYWYAYIAIFIPGLGWSLDNPSRRSLIMDMLGSRGITNGVALDSV
                     GMHVSKMVGPALAGAMIFLVGVGGSYLMLTSFMIVGCLMILKVSQSKNRIDVANDGDD
                     RGGISQIYANLAEGFKYAFTNQTISAVIVITILMNLLLFPYMQMVTVISKEILNVGPL
                     LMGILMASDGLGALMGSIGIASQSRMKYHGRIYLYGSLLSLVALFLFSNSSWYFLSLL
                     VLLFLGIGTSGFGTMQSTIVLLVSKAELRGRVLGVVTIAIGAGPIGSLIIGAISEWIG
                     APNALMINSVIGFLLVATAGLFMPSIREKIIPFVEAEAVNQPTRSGLSTKV"
     gene            2957..4096
                     /locus_tag="QF838_06845"
     CDS             2957..4096
                     /locus_tag="QF838_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60433.1"
                     /translation="MEIKKLETFQLSAPLEISFGWSQDTISSRSVGLVKITTDDGIVG
                     WGEGCGGPSAAVVEDILAPLIIGENPINRLKLWQKMFHSLYNANLAVGYGGSAISAID
                     TALWDITGKILGLPIYDLLGGQIRNSVPVYATGLYYTEGEMPTRLLAEARGYVESGFI
                     GMKTKIGGLKIEDDVKRVKAIRDEIGPDIKLMVDANQAYNASSAIRIGQKLAHLDLVW
                     FEEPVNAQDIDGYLSVKSSLPMAIAGGENLRTRYEFQQFLSRRAYDIVQPDIINVGGI
                     SEMRNVAMTANTMGIQVNPHVWGSPIMIAASLHVASTLPPCPPSGNPEPFVQETVMEY
                     DRTPSAIRDDISPETFVLTNGRLEVPTGPGLGITVDEKAVRSLSS"
     gene            complement(4138..5235)
                     /gene="ychF"
                     /locus_tag="QF838_06850"
     CDS             complement(4138..5235)
                     /gene="ychF"
                     /locus_tag="QF838_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009801701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HJO60434.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKTAMAQAENYPFCTIEPNVGE
                     VAIPDPRLYELADIAKSENIVPTRMTFVDIAGLVKGASKGEGLGNQFLANIREMDAIA
                     YVLRCFDDENIVHVEGKIDPISDLETVETELMLADLETLEKREYSLEKKAKSGDKEAQ
                     DILGLVERALVLLREGRPVRMMDIKPVDEALFKSLQLLTAKPVLYVCNVDESDASQGN
                     QYSQLVFDTAKKEGAGAVLISAKIESELSQLESNDQQEFLQELGLEEPGLVRMIYEGY
                     DLLDLMTYFTAGPKESRAWTVPAHSLAPRASRAIHTDFEKGFIRAEVISFSDYVQCDG
                     EQGAKEAGKMRVEGKDYEVQDGDVVLFRFNV"
     gene            complement(5440..5586)
                     /locus_tag="QF838_06855"
     CDS             complement(5440..5586)
                     /locus_tag="QF838_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HJO60435.1"
                     /translation="MEAYCVKCRAKVEINNPQQVTLKNGRPATKGICSNGCGTNVFRI
                     GKAS"
     gene            5896..7107
                     /locus_tag="QF838_06860"
     CDS             5896..7107
                     /locus_tag="QF838_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HJO60436.1"
                     /translation="MDSGTTAWMLTASALVFFMTPGLAFFYGGLVRAKNLVNTIMYSF
                     ISICVVSIVWVLWGYSLAFGEGNNFVGDFSMVGLNGIALFAAEGDAYPPLVDVIFQMM
                     FAIITPALITGALVERFKFSTYLIFLVIWSTVIYSPVTHWVWGGGWISDMGALDFAGG
                     TVIHINAGIAAVAAAMLVGKRRNPGLQPNNVPYVVLGAAILWLGWFGFNAGSALAADS
                     YAVNAFLVTNVAAATAGLTWGLISMIKTGKMGAVGVATGAVAGLVAITPAAGAVDAMG
                     ALAIGFGAGLICFFAVELIHKTGMDDALDVFAVHGIGGIWGCLATGIFTVSALAGVDG
                     LLGGSAKQLGIQAISAFGTLAYSGIATFIILFILDKIPGLGLRESEADEDQGLDLATH
                     GEQGSVNDGAN"
     gene            7286..7630
                     /locus_tag="QF838_06865"
     CDS             7286..7630
                     /locus_tag="QF838_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HJO60437.1"
                     /translation="MLKIEAIFRPERVSAVAESLLAAGVTGFHYQNVTGQGQQKGVEV
                     VTGRGGQTTTRSASSKTLLVTVISNDKKDAVVEAIVSASRTGEIGDGKIFVTEISDTI
                     RVRTGEAGESAI"
BASE COUNT         2343 a         1731 c         1676 g         2099 t
ORIGIN      
        1 ctagatgtta gcgcctactc tcttgtgtca atatccaggc atgcttccca actaatgact
       61 gaaggtggaa atatcattac catgtcattt atggccgcag agaaagtctt ccccggttac
      121 aacgtcatga gcacagcaaa agcagcccta gaaaatgaag tgatgcaatt agccaatgat
      181 ctcggccccg acggaataag agtaaacgct atctcagcag ggcctctgga caccttatcg
      241 tccagagtga taagtgggta tagagacatg aaaaaggcac atctagaaag ggctccgatg
      301 aaaagaaata taactcatga tgaagtggcc tctactgctc tatatctttg tagcgatatg
      361 tctagtggtg tgaccggtga ggtagttcac gtcgacactg gctatcatgt aatggggata
      421 tagtaatgcc ttgggcgcaa accattgtag atgctctcaa agaacatgaa ataagtttga
      481 tagcctacgt tcccgattca agtattcacc aagtcaccaa gatagtggat aaggacgaat
      541 atttccattt ggtctcagca acacgcgagg aggaagcttt aggaatcgca gccggagctt
      601 acgctactgg aagaaatgct gccgttttca tgcagtccag tgggtttggc aattctatta
      661 acgctgtggc aggacttgct ataccgagcc gaattcccat tccgatgttc attaacctga
      721 gaggggagca tggtgagttt aatatcgcgc aggtaactat gggccgatct acaagaccca
      781 tattagatgt gctcggcaca ccgcattaca ctttggatag cgaggagaga ctcaaagaaa
      841 aggtcaaagg agctttaaaa ttatgttatg catcaaggga acctttggcc ctttgtatga
      901 cacctctact tcatggaggt aaaaatgttt agatattccg ccaccaaaaa aataataggt
      961 aatttgaaga gagaaccggt tgtatccaac ctgggaccga catctgatga gctatggcat
     1021 gcttcagata gagacagaaa tttttacacc tacgggtcta tggggctctg ttcatccatc
     1081 gcgctaggaa tggccttatc ctgcgatgaa aaagtcattt cactggatgg tgacggatcg
     1141 atattaatga atcttggaac gatagcaacg attggacgag aagctccggc taatctcata
     1201 gttattgtct gggataacga gcaatgggct cagaccggcc atcagaaaag ccataccgca
     1261 tttggaacag atttagaact ggtagccaag tccagcggaa tcgaaaaatc ctttacagta
     1321 gcggacgagg aagagttaga ctcaattctt ttgcaagcgt tgaacgaaga tggaccatgg
     1381 ttcatagtag ccaagataga ggatgaaccg tacctttcag tggcacctgt cgaaccagaa
     1441 cacactctcc accgatttcg tcaaaccttc gtcgatagac cagaccgagt cggctgattc
     1501 accgcttctg cttcaacaaa gggaatgatc ttttccctta tcgacggcat aaatagacct
     1561 gctgttgcaa ccagcagaaa tccaattact gaatttatca tcaaggcatt aggggcacca
     1621 atccattcag agatagctcc tataattaaa gatccgattg ggcccgcacc aattgctatg
     1681 gtaacgactc ccaggactct tcctctaagt tccgccttag aaacaagcaa gactatagtc
     1741 gattgcatgg ttccgaatcc agatgtacca attcccaaaa acaataacac caggagcgat
     1801 aaaaaatacc agctggaatt ggaaaataga aacaatgcca caagggacaa taaagaacca
     1861 tacaaatata ttctgccgtg gtatttcatt ctgctctgtg atgctattcc aatagagccc
     1921 attaatgccc ccaacccatc actagccatc aaaataccca ttaacaaagg ccctacattt
     1981 aatatctctt tagaaatcac agtcaccatc tgcatgtagg ggaaaagaag tagattcatt
     2041 aatatcgtaa tgacgataac agcagaaata gtctggttgg taaaagcata tttaaaccct
     2101 tcagccaagt ttgcatatat ctgggaaatt ccgccgcgat catctccgtc gttcgcaaca
     2161 tcaattctat tttttgactg ggaaaccttc aaaatcatta ggcatccgac aatcataaat
     2221 gaggtaagca tcaagtagga tccaccaacg cccacgagga aaatcatcgc tcccgcaagt
     2281 gctgggccga ccattttgct tacatgcatt cctacagaat ctaaagccac tccattggtt
     2341 attcctctgg acccgagcat atccataatt aaagaccttc gggaggggtt gtctagagac
     2401 caacctaacc ctgggataaa tatcgctata tacgcatacc aatattccac agacccgaat
     2461 ataaatagca gagacattac ggtagcagcc gccaaattaa gacattgagt cgcaactatt
     2521 aattttttgc ggtttaattt atcagcaagg aatcccccaa aaataccgag aaccagaaaa
     2581 ggtaccatcc caaagaaacc caccagtcca accgaaaatg gtgaccttgt ttgttccaag
     2641 acgaaccacg ataaggtggt catttgcatc catctagata catacatgga aatattcgtt
     2701 ccccacagaa ggcgataatt actattccga aaggaatcga acgtatgcaa atttcggata
     2761 tttttcgtat ttattcgtga attattcgac atgaattatt agtcatatat tctacagctc
     2821 acacgcaaca cttcaagagt taagcaaaac aacatattat taaaaagaaa tcttaactgg
     2881 aatgggaaaa gccaagttga tgggacaata gttgcttctt agcatctcaa aaaaggccag
     2941 gaatttggag aagtttatgg aaattaaaaa attggagaca ttccaactta gtgctccgtt
     3001 agagatatcc ttcggatggt cacaagacac aataagcagc agatctgtag gtcttgtaaa
     3061 aatcactacc gacgacggca tagttggatg gggagaggga tgtggtggcc cgtcagccgc
     3121 agttgttgaa gacatcttgg ccccattgat aataggtgaa aatccgatca ataggttgaa
     3181 attgtggcaa aagatgttcc attccctcta caacgctaac ctcgccgtag ggtatggggg
     3241 atctgccatc agcgccattg acacagcttt atgggatata acaggtaaaa ttctaggact
     3301 ccctatatac gacctcttag gtggccagat cagaaatagt gtccctgttt atgctacagg
     3361 tttgtactac actgaaggag aaatgcctac tcgtttatta gctgaggcta gggggtacgt
     3421 ggaatccgga ttcataggaa tgaaaacaaa aataggtgga ttgaaaattg aagacgatgt
     3481 aaaaagagta aaagctattc gagacgaaat aggccctgac attaaattaa tggtggatgc
     3541 aaatcaggct tacaacgcat cttcggccat aagaattgga caaaaactcg cacatctgga
     3601 cctcgtttgg ttcgaagaac cagtaaacgc tcaagatatc gatgggtatt taagtgttaa
     3661 atcttcatta cctatggcta tagccggggg cgaaaatctc cgcacaaggt atgaatttca
     3721 gcaattttta tctagaaggg cttatgacat agtccaacca gacataatca atgtcggtgg
     3781 gatctcagaa atgagaaatg tggctatgac tgcaaacacg atgggaatcc aggtgaaccc
     3841 tcacgtctgg ggatctccca ttatgattgc agccagtcta cacgtagcct caacattacc
     3901 tccatgcccc cccagcggaa atcctgaacc atttgttcaa gaaacagtaa tggagtacga
     3961 cagaacacca agtgccattc gggacgatat cagtccagaa acattcgtat taactaatgg
     4021 tcggttagaa gtgccaacag gcccaggatt aggcataaca gtagatgaaa aagctgtacg
     4081 gtctctttcc agctgattta attcaataat accgccagag aaagatggct ttgattgcta
     4141 cacattgaat ctaaataaaa ctacgtcgcc atcttgaacc tcataatctt taccttccac
     4201 gcgcatcttt ccagcctctt ttgctccctg ctctccatca cactgaacat agtctgaaaa
     4261 agaaataacc tcggctcgaa taaatccttt ctcaaagtct gtgtgtatag ccctggaggc
     4321 tctaggggcc agagagtgag caggaactgt ccaagctcta gactctttcg gcccagccgt
     4381 gaaatatgtc atcaaatcta ataggtcata accttcatat atcatcctca ccagccccgg
     4441 ctcctctaat ccaagttcct gcagaaactc ctgctgatca tttgactcaa gttgggatag
     4501 ttcagattct attttggcag agatcaaaac agcacctgcc ccttcttttt ttgctgtatc
     4561 aaaaaccaat tgcgaatatt gattcccctg agaagcatct gattcatcaa cattacaaac
     4621 atataaaaca ggtttagcag tcaataattg aaggctctta aataaagcct catcaacagg
     4681 cttaatatcc atcattcgta ctgggcgccc ttctcgcagc agaaccaatg ctctttctac
     4741 aagccctaag atgtcctgtg cttctttatc tccacttttt gctttttttt ctaacgaata
     4801 ttctcgtttt tctagtgttt ccaaatctgc gagcatcagc tcagtttcca cagtttcaag
     4861 atccgaaatg ggatctattt ttccttctac atgaacaata ttttcatcat caaaacatct
     4921 cagcacataa gctatagcgt ccatttctct gatatttgca aggaattgat ttcccaaccc
     4981 ctctccttta gaagcccctt tcacaagtcc ggctatatcc acgaaggtca ttctagtagg
     5041 aactatgttt tcggatttag ctatgtccgc cagctcgtat aacctgggat ctggtattgc
     5101 cacttctcct acgttcggct caattgtgca aaaggggtag ttctcagctt gagccattgc
     5161 cgtcttcgta agagcattaa aaagggtgga tttgccaaca ttaggcaacc ctacaattcc
     5221 acatttaaat cccattagct aaatccaata ttccatagtt ataatcccca gagagcctac
     5281 tataagcata aattggtcgt gtttggaaaa atagtaacaa aaaaaccccg acaaagcggg
     5341 gctaaactct tcctttctaa ttggaagaat cattccaaaa cgacacagtc agggtggatt
     5401 taactaaacc caccccaagg tgctaagaat tctgcagtgc tagctggcct taccaattcg
     5461 gaaaacgtta gttccgcatc cgtttgagca aattcccttc gttgcgggtc taccattctt
     5521 caatgtaacc tgctgagggt tattaatttc aaccttggcc ctgcacttta cgcagtacgc
     5581 ttccatatct ccctccttgt gaggactaac tatatagttc atattattca tataatttaa
     5641 cttaacgcaa ccctttttct accatatatg gtactttctt tctgcattta gcctgctaca
     5701 tatggtattt ttgcccttat agggtggaaa attacagatg tctgaatagc ccaagacata
     5761 tttctgtaac acacaagcaa catctattta acaacagggc aatacttaag aaacatcaat
     5821 tccatagact tcaatttaaa taattcaggc gaattagtca caccgaaatt attcgccgta
     5881 atacggaggt ttaatatgga ttctggaaca accgcctgga tgcttacggc atccgccctg
     5941 gttttcttca tgactcccgg gctcgcattt ttttacggtg ggctagtaag ggccaaaaat
     6001 cttgtcaaca caataatgta cagcttcatt tccatttgtg tggtgagtat agtctgggtc
     6061 ctttggggct acagtctcgc atttggggaa ggaaataatt tcgtgggaga tttctcgatg
     6121 gtagggctaa atggaattgc cctatttgcc gcagaaggag acgcctaccc cccattggtt
     6181 gatgttattt ttcaaatgat gtttgcaatt atcactccag cccttataac tggggcttta
     6241 gtggaaagat ttaaattttc cacctactta atattcttag tcatatggag tacggtcatc
     6301 tactctccag taacacattg ggtgtggggt ggtggctgga tttcagatat gggagctcta
     6361 gattttgctg gtggaacagt tatacacata aacgccggta tcgctgcagt ggctgcagcc
     6421 atgctggtag ggaagcgccg aaatcctggc ctgcaaccga ataatgttcc gtacgtcgtt
     6481 ttaggcgcag caattctatg gctaggatgg ttcgggttca atgcggggtc cgctctagca
     6541 gctgacagtt acgcagtaaa tgcttttctc gtaaccaatg ttgctgccgc aacagccggg
     6601 ctaacgtggg gacttatttc catgattaaa actggcaaaa tgggcgctgt tggtgtggca
     6661 actggagcag tagccggatt agtcgccata actcctgcag caggagctgt tgacgcaatg
     6721 ggtgccctgg ccataggctt cggtgctggc ctcatttgtt tctttgcagt ggagttgata
     6781 cataaaactg gaatggatga tgcattggac gtatttgctg ttcacggcat cgggggaatc
     6841 tgggggtgtc tagctacagg aattttcaca gtaagcgcac tcgcaggggt agacggatta
     6901 cttggtggca gtgcaaagca actaggaata caagcaatca gtgcttttgg aacgctagca
     6961 tactctggaa tagccacttt tataatacta tttatcctgg acaaaattcc gggcttaggc
     7021 cttagggaat cagaagccga cgaagaccaa ggtttagacc ttgcaaccca tggagaacaa
     7081 ggttctgtaa acgacggagc taattaaacg aaataacggt tcaatctatc gatcatcgaa
     7141 aagtcgccga tatgaggttt tttggtgatc gataacagta ttgtgattgt tacacaaatg
     7201 tttcctaaat attgccaaaa tattacacca tgtgtaatat ctgatccgta aatgtttcca
     7261 caatgttaca cgaggaggaa gattgatgtt aaagatagag gctatttttc gacctgaacg
     7321 agtgagtgca gttgctgagt ccttgctggc tgccggtgtt accgggttcc attatcaaaa
     7381 tgtcacaggc caagggcagc aaaaaggagt tgaagttgtt actggcagag gagggcaaac
     7441 cacaacacgc tcagcgtcat ccaaaactct tcttgtcacc gtgatctcca atgacaaaaa
     7501 ggatgctgtt gttgaagcaa ttgtttctgc ttccagaact ggcgaaattg gggatggaaa
     7561 gatatttgtg actgaaattt cagatactat acgtgtcaga acgggagagg ctggggaatc
     7621 tgcaatataa caagtaaagg tacttccaca cttgtagttt cgtccaactg ttgaagagat
     7681 cttgcagcat aggtattatc tgtagcaatg cctactaacg tgccacctca atacagggat
     7741 gcggaacaaa gatttcgcga agctgtctca gtgcaaggca aaatagcggc tttacaagaa
     7801 atgctccaga ttatgcctaa acataaaggc acagatcatt tgaaggccc
//
LOCUS       DASCNS010000192         8047 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001527504, whole genome shotgun sequence.
ACCESSION   DASCNS010000192 DASCNS010000000
VERSION     DASCNS010000192.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001527504"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="QF838_06870"
     CDS             complement(<1..307)
                     /locus_tag="QF838_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HJO60438.1"
                     /translation="MKHVIVYKEVGRYAGWPANYGIWSWGDEIVTGFTLGFHNNEGGF
                     HFRDKDRPFMTMQSRSTDGGLTWDTGQAPLSAPGDVAICADEHMNLPLGPVHSRTNPP
                     "
     gene            complement(383..820)
                     /locus_tag="QF838_06875"
     CDS             complement(383..820)
                     /locus_tag="QF838_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJO60439.1"
                     /translation="MPKLTRDEMEEFLTTGSKILKLGTLTEDTYPYVNPLWYTYENDV
                     FLVAGRSKALWVNHIKRNTKTSLCIDTYIAPYTRVIAETDAEILDAEWTGDWEHWAHR
                     YLGEKEGHQYYEDTKHMPRVLVQLTPKKLSTWAGPGWHPRYQE"
     gene            complement(838..1377)
                     /locus_tag="QF838_06880"
     CDS             complement(838..1377)
                     /locus_tag="QF838_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60440.1"
                     /translation="MNKEQGVWIISGVPAAGKTTTAKSLAKTFDKAAVINRDDLQAQI
                     ISGSVWPDGIPTTEANLQIGMNVRNQCALANSYLSSGFTPVCEEVVGRYQLEIYQDLL
                     DSENIYLVTLDPPYDVVIKRDKYRETQEQFNNPERCKLLYEFIKTVCGYGLWFDNSQM
                     TVGQSVKHILDNKDTCLIG"
     gene            complement(1398..3881)
                     /locus_tag="QF838_06885"
     CDS             complement(1398..3881)
                     /locus_tag="QF838_06885"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60441.1"
                     /translation="MSLGNTQVLSDLRIIEIGRDVSTAYAARFFAIYGAEVIVVEPPE
                     GHDIRWQPPWPEDIPDPEKSLLFAYLGGGKKSVVIDFDSKSDMDILRNLISSSDGVLD
                     SYPPGYLASYGLDLNEMCDLKKDLSVTQISPYGQFGPQANWKASSITAAASGGQMYLA
                     GDIDKPPLFTVGHQAYYQGGVQGFGALLAGIYSSKSTGIGEIFDLSIQEIQAATLEGG
                     GPSAMWFGGEQTRSGNNPRALWGIYECLDGWVGVASMPRQTKSVLDAMGYSEMKKDPI
                     FNTGGWNQESDDLLRVLVPAFTATKTAKEIFDIADQHRAPFGMIPTPKDLIEWPNHQI
                     TGFWNKVDHPLLGTHLYPSGPIGFDGDRGEFEPAPSIGQHTDELKERLSKREASKALS
                     FKPKDLSMPLEGIRVVDMTQVWSGPYGCRFLADMGADVVKVEGPTFPDPVRTAGGARE
                     TPEIDLAPYFNEYNRGKKGLSLDFKKPEGMKILRSLVATADVFVENWSSGVAERNSLS
                     YEDLKAIKPDLVYISMPGFGHEGPDSSRVGFGPTIEQMGGLVALQGYEGGAPHKSGIS
                     YGDPIAGSTCAASVIASLVYREKTGEGMYCVIPQRDGVTGLVGEYFVAESLGIELPLR
                     TSPRHLKSAPHNVYETLPDKEGRPILGLLARSPNPEIVDYAFDRWITIDCQTDEDWEK
                     IVEIIADERLGDEKFLEAANRRKYVTEIDEVISEWAKNQNAEEISRALQGAGVCAAPV
                     MSPALLMKDEHMLERESFIKVVHDVAGEHLVSRPSWRAKRRTELPSKSGPCFGADSDE
                     ILGELGYSKKDVEDLYEKGVTSRSLLGGR"
     gene            complement(3904..4422)
                     /locus_tag="QF838_06890"
     CDS             complement(3904..4422)
                     /locus_tag="QF838_06890"
                     /inference="COORDINATES: protein motif:HMM:NF016840.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HJO60442.1"
                     /translation="MDFREILKLQFDEYQSETERYLDGLSDGERRFMPFEKYNHIEFI
                     LWHASRAEDALLNSSVLEEEQLWIRGGWSEKFGIPPADTGLGYTAQQVKDMPAVSLGD
                     LLAYQKAVRAETLECVRTITPGEMDKRCPNERVHRKLPEVTKGGILAHMVVEPSQHLG
                     QIGYIRGIIRGM"
     gene            complement(4640..5158)
                     /locus_tag="QF838_06895"
     CDS             complement(4640..5158)
                     /locus_tag="QF838_06895"
                     /inference="COORDINATES: protein motif:HMM:NF016840.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HJO60443.1"
                     /translation="MDFREILKLQFDEYESETERLVDGLTEEERRFMPSEESHHIDFA
                     LWHAARAEDILLNLGVREEEQLWVRGGWAEKFGIPPADVGVGYTAQQVKDMPAISLED
                     LLAYYKAVRAETLECVRTIAPEEMDKRCPFERLHHQLPEVTKGGVLAHMVVEPVQHLG
                     QIGYIRGIMRGM"
     gene            5272..6021
                     /locus_tag="QF838_06900"
     CDS             5272..6021
                     /locus_tag="QF838_06900"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO60444.1"
                     /translation="MRLEGKTAIVTGASNGMGSSESKLFAKEGANVVLADVDSKAGQQ
                     LVEEISEGGGQAHFEMCDVSSEDNWQSMSDNVLSKYGSVDILVNNAGISSTSTSDLLS
                     TDDWNNIMNVNSTGVFLGTKTAAEIMKSQGGGSIVNISSIMGFVGGEYGHPAYHASKG
                     SVRIFSKAMAVRYGPYAVRVNTVHPGFMPPMTSSNHGFASKRDEYVSQTPLRRTGESI
                     EVAYGVLFLASDEASFVTGTELVIDGGYIAQ"
     gene            6087..6980
                     /locus_tag="QF838_06905"
     CDS             6087..6980
                     /locus_tag="QF838_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO60445.1"
                     /translation="MSRINKCIELIEQNQAIFAVSAPELTYECGKEMSQTWADLIQFD
                     FEHHPFDTVGLSKFMKGLKDGGPTPSGHLTPTVITTVPSNCMTPEEVIYNAWQIRHVL
                     STGVHGILHTHTRRADAVATFVAASRYVFQTIGRDQGIPEGLRGGGGQKEPAAIWDMD
                     PTDYMRKADPWPLNPNGELLLGLKIEDRFCLPDADNIAAVPGISFAEWGPGDMGMSHG
                     DPDLHDPPYTQEMDEARNIVKNACDKSGLTFLCSWQDENLTIEERARHALHEIGARIL
                     HVPDEAIADKLRKEMDRKMPW"
     gene            7053..7925
                     /locus_tag="QF838_06910"
     CDS             7053..7925
                     /locus_tag="QF838_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60446.1"
                     /translation="MKEPKTIEIPIKNYQNTIYEWEGNSPTLLFCHATGFHGRIWNRV
                     IEKLNGYHCISIDLRGHGKSTKTEPPYPWDSFVPDVMEIIRVLKLSNIIGIGHSMGGH
                     VITSVASKMKGLVNGLVLCDPSLFVDHRYKKKKPNVEPDYSHPVSRRRNRWERPSEMY
                     DRLIQHQNFASWEPAVLMDYCIYGLERYDDSYRLSCPPSVEAAMYGDYIDPKVLQDLK
                     TYENPVSILLARVMKSTESFDDFGPSITRNDLGNMFPDAVVKRYSKHSHFLPMENTKL
                     VADAIMQHCEILTK"
BASE COUNT         2251 a         1865 c         1671 g         2260 t
ORIGIN      
        1 ctggtgggtt cgttcgggaa tgcacgggac cgagtgggag gttcatgtgc tcgtctgcac
       61 aaatagcaac atccccagga gcagataatg gtgcctggcc agtatcccag gttagcccac
      121 cgtcggtgct gcgggactgc attgtcataa aaggtcgatc tttgtcccta aagtgaaatc
      181 cgccctcatt gttatgaaat cctaatgtga acccggtgac aatctcgtct ccccagctcc
      241 agatgccgta gttggcaggc cagccagcat atctcccgac ctctttgtat acgatgacat
      301 gtttcatggc tctatcttaa ctcaagcgta cggatcaaag agaaaccagc tagtcttgca
      361 gcaaagaggt tacagactga ggctattcct gataccgtgg gtgccagccg gggcctgccc
      421 aggtagaaag ttttttagga gtcagctgca ccagtactct cggcatgtgt ttggtgtctt
      481 cgtagtactg atgaccttct ttttccccta gatatctgtg agcccagtgt tcccaatccc
      541 ctgtccattc agcatcaagg atctcagcgt ctgtttctgc aataactctt gtgtatggag
      601 ctatataagt gtcgatacac aatgatgttt tcgtgtttct tttaatatga ttcacccata
      661 gtgctttact tcggcctgct acaagaaaca catcgttttc ataggtgtac caaagtggat
      721 ttacatacgg ataggtatct tcagtaaggg tacccagttt cagtattttc gatcctgtag
      781 tgagaaactc ttccatttca tctctggtaa gtttcggcat ttgccattcc tcctaatcta
      841 gccaataagg catgtatcct tgttatccaa tatatgtttg actgactgtc caactgtcat
      901 ttgtgaatta tcgaaccata gaccataacc acacacagtt ttaatgaatt catacagcaa
      961 tttacatctt tccggattat tgaattgttc ttgagtttct cgatatttat ccctttttat
     1021 cactacatca taaggagggt ctaaagtaac gagataaatg ttctctgaat ccaataagtc
     1081 ctggtatatc tccaattggt atctgccgac aacctcttca cataccgggg tgaatccact
     1141 ggatagatat gaattggcta gggcacattg attccggacg ttcattccta tttgtaagtt
     1201 agcctccgtg gttggtattc catcgggcca tacactccca gaaattatct gagcttgcaa
     1261 atcgtctcta ttaatgactg ccgccttatc aaaggttttt gcaagtgatt ttgcagtagt
     1321 agttttaccg gcagcaggaa ccccgctaat aatccatacc ccctgctctt tattcatcac
     1381 aagccttcct aaataattta tctacctcca agcaaactac gggaagttac ccccttctca
     1441 tataggtcct ctacatcttt ctttgagtaa cctagctcac ctagtatttc atcactatcc
     1501 gcgccgaaac aaggccccga ttttgacggc aattcggtgc gtcttttcgc acgccatgaa
     1561 ggtcttgata cgaggtgttc tcccgcgaca tcgtggacaa cttttataaa actttctctc
     1621 tctaacatat gttcatcttt catcaacagg gctggagaca tgactggtgc agcgcagaca
     1681 cctgcgcctt gaagtgctct agagatctct tcagcatttt ggtttttggc ccattctgaa
     1741 atcacctcgt cgatttcggt gacatatttt cttcgatttg ctgcttccag gaatttttca
     1801 tcacctaaac gctcatccgc aataatttca acaatctttt cccaatcttc atctgtctgg
     1861 caatcgattg tgatccatct atcaaaggca tagtcgacaa tttccgggtt tgggctccgt
     1921 gctaaaaggc ccaatattgg tcttccttct ttatcgggga gggtttcata cacattgtgt
     1981 ggtgctgatt taaggtgcct gggactagtt cgtaaaggca attcaattcc tagagattca
     2041 gcaacaaaat actcacccac caatccggta acaccgtccc tttgagggat tacacagtac
     2101 atgccttcac ctgttttttc tctgtacacc aaagaagcta taacagacgc cgcgcaggtc
     2161 gaaccagcaa taggatctcc ataagatatt cctgatttat ggggtgcccc tccctcatat
     2221 ccttgcaaag ctactagtcc acccatttgt tcgattgtgg gtccgaaacc taccctcgaa
     2281 gaatcaggcc cctcatggcc aaatcctggc atagatatat agactaaatc aggttttatt
     2341 gcttttaggt cctcatatga caaactgttc ctttcggcaa ctcctgaaga ccaattttcg
     2401 acaaatacat ccgctgtcgc caccaggctt cttaggattt tcataccttc tggcttctta
     2461 aagtcgagtg ataacccctt cttccctctg ttgtattcat taaaatatgg ggcaagatct
     2521 atttctgggg tttcgcgagc acctcctgct gttctaactg gatcaggaaa tgtaggcccc
     2581 tcaactttta cgacatcagc tcccatgtcc gccaagaatc tgcacccgta agggcctgac
     2641 cacacctggg tcatatcaac taccctaata ccctctaatg gcatggaaag atctttcggt
     2701 ttaaaagata aggctttaga agcctctctt ttagaaagcc tctcttttag ctcatccgtg
     2761 tgctgcccta tgctaggagc cggttcgaat tctcccctgt cgccatcaaa gccgataggg
     2821 cctgaagggt ataagtgagt ccccaataac ggatgatcaa ccttattcca aaaaccagtt
     2881 atttgatggt taggccactc aatcaaatcc tttggtgtcg gtatcatccc aaatggagca
     2941 cggtgctgat ctgctatatc aaaaatctcc ttcgcagtct tggttgcagt aaatgcaggg
     3001 acaagtactc taagcaaatc gtctgactct tgattccagc ccccagtatt aaatataggg
     3061 tcttttttca tttcagaata ccccatggcg tctaatacgg atttagtttg tctcggcatt
     3121 gacgcaacac ctacccaccc atccaaacat tcataaatac cccaaagagc tctgggatta
     3181 ttaccacttc gggtctgctc tcccccaaac cacatagcgg agggtccgcc tccttctaaa
     3241 gtggccgcct gaatctcttg tatggacaaa tcgaaaattt cgccgatccc agtgctcttg
     3301 ctagaataga tgccagctaa tagggcacca aacccttgaa caccgccttg atagtaggcc
     3361 tgatggccta cagtgaaaag aggtggttta tcaatatctc cggccagata catctgacct
     3421 cccgatgctg ccgctgttat tgaggatgcc ttccaattgg cttgaggtcc aaattgtccg
     3481 tatgggctaa tttgggtcac tgacaaatct ttcttcaaat cgcacatctc gtttaaatct
     3541 aatccatagc tagcaagata ccctggagga taactatcta gaactccatc cgaagatgat
     3601 atgagatttc gtagaatatc catatcagat ttactgtcga aatcaatgac aacagacttc
     3661 tttccaccac caagataggc aaacagcaaa ctcttttctg gatctggaat atcttcaggc
     3721 catggcggct gccatcttat gtcatggcct tctgggggtt ctacaacaat tacttcggcc
     3781 ccataaattg cgaaaaacct tgcagcatag gcagtggata catccctgcc aatctcgatt
     3841 attcttaaat cacttaaaac ctgagtatta cctaaactca cgtgttcacc ctttctgaga
     3901 aagttacatt ccccgaataa ttcctcgaat atatcctatt tgacctagat gctgagatgg
     3961 ctcgacaacc atgtgggcta aaatgccacc tttagtgact tctggtagtt tgcgatgcac
     4021 acgttcatta ggacatcttt tgtccatttc accgggggtg atagtccgta cacattcaag
     4081 tgtttctgcc ctaacagcct tctgataggc aagtaaatca ccaagagaaa ctgccggcat
     4141 atcttttact tgctgagccg tgtatccgag gccggtgtcg gccggcggga taccgaattt
     4201 ttctgaccaa ccacctctga tccatagttg ctcttcttct agaacactgg aattcaaaag
     4261 agcatcctca gctctagaag catgccataa aataaactcg atgtggttat atttttcaaa
     4321 cggcatgaag cgtctttcgc cgtcgcttaa tccatccaga tatctttctg tttctgattg
     4381 gtattcatca aattgaagtt tcaatatttc cctaaaatcc atagatgcac ctcttagtca
     4441 aggtattggt ctttttctga catttaccaa gatccaaagc ataatacaaa atatgtaatc
     4501 gttgggctat accagtattc gtgtctggag gaaaattgag acggtaaact catcatgtag
     4561 cttcgatggc gccacagaaa cgaatccctt tgcgtaccgt agcttcactc tcagtacgat
     4621 acttttccct gtcacagggc tacattcccc gcataatccc tcgaatatat ccgatttggc
     4681 caagatgctg aactggttcg acaaccatat gggccaaaac cccgccttta gttacttctg
     4741 gtagttggtg atgtaaacgt tcaaaaggac atcttttatc catttcctca ggggcaatgg
     4801 tccttacgca ttcgagtgtt tccgccctga cagccttata ataggcaagc agatcttcta
     4861 aggaaatggc cggcatatct ttcacctgct gggcagtgta tccaactccg acatctgccg
     4921 gagggatccc aaatttttct gcccagcctc ctcttaccca caactgttcc tcctcacgaa
     4981 cgcccagatt caataaaatg tcctcggcgc gggctgcgtg ccataaagca aaatctatat
     5041 gatgagattc ttcagaaggc ataaaacgtc tttcttcctc tgttaatcca tctaccaatc
     5101 tctcagtttc agattcatat tcatcaaatt gcagtttcaa tatttcccta aaatccatag
     5161 aatcaccttt aattagagtt tttgtatctt cacgatattt ttgaaaattc agagcatgat
     5221 acaaaataaa cattgaatgg gctacaaaag aattcttgtt tggaggtcag tatgagacta
     5281 gaaggtaaaa cagccatagt cacaggagca agcaatggga tgggttcgtc agaatccaaa
     5341 ttatttgcaa aagaaggagc taacgtcgtt ttagccgacg tagattcgaa agcaggacag
     5401 cagttagtgg aagaaatatc tgaaggcggt ggccaagcac actttgaaat gtgtgatgtt
     5461 tcaagtgaag ataattggca atctatgtct gacaacgttc tgagtaaata cggctccgta
     5521 gacatattag taaacaacgc tggaattagt agtacttcta cctctgactt attaagtact
     5581 gatgactgga ataacataat gaatgttaat tcaaccgggg tcttcctagg gacaaaaacc
     5641 gccgcggaaa ttatgaaaag ccaaggcggt ggatcgatag tcaatatatc ttcgataatg
     5701 ggatttgttg gaggagagta tggtcatcct gcatatcacg cctccaaggg ttcagtacga
     5761 attttcagta aagccatggc ggttaggtac ggcccatatg ctgtcagggt aaacacggta
     5821 catcccggat tcatgcctcc tatgacatca tctaatcatg gcttcgcaag taagcgagat
     5881 gaatacgttt ctcagacacc tttaagaaga accggagagt cgatcgaagt cgcctatggc
     5941 gttctattcc tggcctcaga tgaggcatca tttgtgacag gcactgaact tgtaatcgat
     6001 ggaggctata tagcccaata agatcaaata attgcgttct gtatagcttc gattagaata
     6061 ttcacaaata agcgggagga ctaaatatga gcagaattaa caagtgcata gaacttattg
     6121 agcaaaatca ggctattttc gctgtttccg caccggaatt aacttacgaa tgcggcaaag
     6181 aaatgtcaca gacatgggcc gatcttattc agtttgattt tgaacatcat ccttttgata
     6241 cagttgggtt aagtaaattt atgaaagggt tgaaggatgg cggaccaact ccaagtggac
     6301 atctcactcc aacagttata acaacagtcc cttcaaattg tatgactccc gaagaagtga
     6361 tctacaacgc ctggcaaatc aggcatgtat tatctacggg cgtacatggt atcttgcata
     6421 cccataccag aagggctgat gctgtcgcaa catttgtggc tgcttctcga tatgttttcc
     6481 aaacgatagg aagagatcaa ggtataccgg aaggtttacg gggtggtggt ggacagaagg
     6541 agccggctgc aatatgggat atggacccaa cagattacat gcggaaagct gacccatggc
     6601 ctttgaatcc aaatggtgaa ctcctattgg gcctaaaaat tgaagatagg ttttgcttac
     6661 cagacgcgga caatattgct gcggtaccgg gaatttcatt tgctgaatgg ggacctgggg
     6721 atatgggcat gtcgcatgga gacccggacc tccacgaccc tccatatact caggaaatgg
     6781 atgaggccag gaatatagta aagaatgcat gtgataaatc agggttaact ttcctatgca
     6841 gttggcaaga cgagaatctc acgatcgagg aaagagcaag gcatgctctc cacgaaatag
     6901 gagccaggat tttgcatgtc ccggacgagg caatagcaga taagttgaga aaagaaatgg
     6961 accggaaaat gccctggtaa cccttgaaag catcgtaatt ggagaccgcg gctacgactg
     7021 cggtctcttt ttttgttaga aggatacaat ttttgaaaga gccaaagacg attgaaatcc
     7081 ccataaaaaa ctatcagaac actatctacg agtgggaagg aaatagccca accctgttgt
     7141 tctgccatgc aacaggtttc catggtcgca tatggaaccg tgttattgaa aaactgaatg
     7201 gctatcattg catatcgatc gatctgagag ggcacggtaa aagtacaaaa acagaaccgc
     7261 catatccttg ggattccttt gtcccagacg tgatggaaat aattagggtc ttaaaattat
     7321 caaatataat aggaatcggt cattctatgg gcggacacgt aataacatcc gttgcttcta
     7381 aaatgaaagg tttggtgaac ggattagttc tttgtgatcc gagtttattt gtagaccacc
     7441 ggtacaagaa aaagaagcca aatgttgaac cagactattc gcatcctgtt tccagaagaa
     7501 gaaatagatg ggaaagaccc tctgagatgt acgaccggct aatacaacat caaaattttg
     7561 cctcctggga gcccgcagtt ttgatggatt actgtattta cggactagaa aggtatgacg
     7621 attcatatag gctctcatgc ccaccttcgg tggaagctgc catgtacggc gactatattg
     7681 accccaaagt gttacaagac ttaaagactt atgaaaaccc agtatcaatt ttattagcta
     7741 gggttatgaa atcaactgaa agcttcgatg actttggtcc gtcaatcaca agaaacgatc
     7801 tagggaacat gttcccagac gccgtagtaa aaaggtattc taaacactca cattttttgc
     7861 cgatggaaaa tacaaaattg gtggctgatg caataatgca acattgtgag atactgacta
     7921 aataaaggac acagccgata tatgtaaagt atcctacaaa cccaaacaga accagttaaa
     7981 tcaagaggtg aaggaatggc atcgagaatc attgaaaaca aagtcactgc ttacctggga
     8041 catgaag
//
LOCUS       DASCNS010000193         5857 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001530965, whole genome shotgun sequence.
ACCESSION   DASCNS010000193 DASCNS010000000
VERSION     DASCNS010000193.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001530965"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="QF838_06915"
     CDS             <1..615
                     /locus_tag="QF838_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019113.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HJO60447.1"
                     /translation="DMFFISAILGRAFKVTDDVSYVNAYVDFLLEAEVQQDDGLFWHS
                     RTTPYFWGRGNGFSALAYSEALTYMPNNYPRRKQILDIHTRHLAGMINNQLPNGTWSQ
                     LIDLPGTYQELSVTCMVGYALARGIRKGWLDSSFMPALEKTWSAAKRRISDNGDLVDV
                     CSGTGFQQKRSDYIYRKAEYGYDDRGGSMAIWFSTEMALLEENS"
     gene            713..1777
                     /locus_tag="QF838_06920"
     CDS             713..1777
                     /locus_tag="QF838_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60448.1"
                     /translation="MNIGVGLDPTLGLDLQQQFELSRESARLGFQSIWTPEGTGEDSY
                     HMCLMRWAASCDVIEGGLETGIAVSPVMWRTPMAFAMAGGTVSKMTDGKFIMGIGAGG
                     AYRPDVRKSINMPNVSALGMMRDYLEIITQLVRGEKVDYEGSVETLINSRLGIRPAPK
                     TPVYLGALGPKMLELAGELADGASLNWCAPEQIAWSRKKIEIGENKSGRKKGSVKVAE
                     YIRICVDEDEEKARVALAKATMNYALGAVVPSDRERTFGYRAHFERMGFTSELAALDE
                     MRKKGASNDEVAEAFPENILRSVAYFGKAENAASEFARLSEGLDNAIVRVVSSRPGTV
                     EGTLDVMNACSPVEVRKHLK"
     gene            1777..2709
                     /locus_tag="QF838_06925"
     CDS             1777..2709
                     /locus_tag="QF838_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012515.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HJO60449.1"
                     /translation="MPDMLSIGESMIELFSEEPMDTAETFRRSVAGDSLNIIVAASRL
                     GTNTGYITKLGDDPFEGYLLDIFSSEGIDTTHVLSSDGFNAVHFVTVMPDGDREFVYY
                     RAGSAPSTIKPEELNPEYISGAKIMHCSGIAQAISPSSRATVLAAAQIAQLNGVPVSY
                     DPNYRHQLWNPSEAKEAMEELMPYVDIFLPSAPADSKVLFETEDPEKVIQEANRRGVE
                     TVVVTLGEDGALIGAENEVMQLDPMPAGPVIDTTGAGDSFKGGFIHGFLEGKSIAESA
                     LIGNVCAGITITGRGALNPMPYRKEVYDKVRNLR"
     gene            complement(2930..3607)
                     /locus_tag="QF838_06930"
     CDS             complement(2930..3607)
                     /locus_tag="QF838_06930"
                     /inference="COORDINATES: protein motif:HMM:NF018038.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HJO60450.1"
                     /translation="MNLLLMSGGRHPYEESTPVLKGFLESAGHTVIVREDAEALTDGT
                     LNKSDVLIFNTLREGDMALDTAQQNALQGYISSGNGFVCIHISGCVPDTWNEYGEITG
                     GGWVMGQSFHPPYSNFSVDVVNGSHPCASSISAFNTDDELYMNIDYRDGNDVFITADF
                     QGGVYGKNRAGAEDMEMEGGTFPLAWTRSYGAGKVFVTLLGHDGKSHQNEGFQKLVLN
                     GVDWATS"
     gene            complement(3641..4789)
                     /locus_tag="QF838_06935"
     CDS             complement(3641..4789)
                     /locus_tag="QF838_06935"
                     /inference="COORDINATES: protein motif:HMM:NF017722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HJO60451.1"
                     /translation="MGRSIHHPVGLIHNSPSSTFNGYTLFSTNGGNHATMIDNLGRVV
                     HRWNYDGGIVYAYLLPNGNLLARTKPPRDVEIVKDLGGSSASLIEMDWDSEIVWEYKN
                     PMLHHDYARLPNGNTSVLLFKELDEEISQQVKGGHPKEDDPKRILGDVIREITEDGKT
                     VNEWEIYQELDFEEDVICPLEHRREWTHANCLNLMPNGDFLISFRNISTVGIISRETG
                     EFLWKWGPGNVFHQHHPTVLENGNILIFDNGSHSQGADRSRVIEVNPITNEIEWEYTE
                     TPAMAFYSFHISSAERLANGNTLICEGAFGRIFEVTKNGNVVWEYINPFYSPDLRSGD
                     PTNMVFRAHRYSPEDPTLDGRDLNPDIYSNINRLYAGSDDNKNRLIVN"
     gene            complement(4835..5680)
                     /locus_tag="QF838_06940"
     CDS             complement(4835..5680)
                     /locus_tag="QF838_06940"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258964427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4961..4963),aa:Sec)
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HJO60452.1"
                     /translation="MATESFANSGILVNTEWLNSHIDDSDIRIVDCDMFDSYARAHIK
                     GAVGIKVHHYIKHPSYSQDSKSYPLVAEPDVVKELFESMGIGDDTTVVSYDSGGSLWA
                     SRFWWVLNYYGHKNAKVLDGGWKKWFDEGRPVSIEPPSPKEVSFTPSADETIICTLDQ
                     AVSKIDDSDVVFLDVRSDGEWDGTNSRGNSRSGRVPGSVHLEWLNFITDDKYHTIKSP
                     SELRDMLKAVGVTPEKEVITYUQGGIRAAHGVFVLTLLGYENKRNYDGSMGEWANRAD
                     TPLTT"
BASE COUNT         1714 a         1301 c         1233 g         1609 t
ORIGIN      
        1 gacatgttct ttatttcggc gatattggga agggcattta aagtcactga tgatgttagt
       61 tacgtaaacg catatgtcga ctttctactg gaagcagagg tccaacaaga tgacggtctt
      121 ttttggcata gcaggaccac tccatatttt tggggaaggg gaaatggatt ctctgcattg
      181 gcctattcag aggctctcac atacatgcca aacaattatc ctcgacgaaa acaaattttg
      241 gacattcata cgaggcatct ggccggtatg atcaataatc aattgccaaa tggaacatgg
      301 tcacaactaa tagatttacc aggaacttac caagaattat ccgtcacttg catggtggga
      361 tatgcactag cgcgtggaat aagaaaagga tggttggaca gttcctttat gcctgcactt
      421 gaaaaaactt ggtcagcagc aaaaagaaga atttccgata atggggatct cgttgatgtc
      481 tgctcaggaa ccgggttcca acagaaaaga agtgactata tctacaggaa ggcagaatat
      541 gggtatgatg acagaggtgg atctatggcc atatggtttt cgacggaaat ggccctatta
      601 gaggaaaata gttgaattaa atttgtgaat cggaattatt tgaagtcatc gtttccttaa
      661 aactacagat agaaaatatg taatttaaac caacttgtta caggagaata atatgaatat
      721 tggtgtggga ttagatccaa ctctgggcct tgacttacaa cagcaattcg aactttcaag
      781 agaatctgca agactcggat ttcaaagtat atggacacca gagggaacag gggaagattc
      841 atatcatatg tgtctgatga gatgggcggc ttcgtgcgat gtgatagaag gcgggcttga
      901 aacaggtatt gctgtatccc cagttatgtg gagaactccc atggctttcg ctatggcagg
      961 gggaaccgtg agcaagatga ccgatggtaa attcataatg ggcattgggg cgggtggtgc
     1021 ttatcgtcct gacgtcagga aatccataaa tatgcccaac gtctcggcac tgggtatgat
     1081 gagggattac cttgaaataa tcactcaact ggttcgggga gagaaagttg attatgaagg
     1141 atctgtcgaa acccttataa actcaaggct tggaatcaga cccgctccaa aaaccccagt
     1201 gtatctagga gctcttggtc ccaaaatgtt ggaattggct ggtgaactcg ccgatggcgc
     1261 atctttgaac tggtgtgccc ccgagcaaat agcgtggagt cggaagaaaa tagagattgg
     1321 tgaaaacaag tcgggtcgaa agaaaggctc cgtgaaagtt gcagaataca tacgcatctg
     1381 cgttgacgaa gacgaggaaa aagcgcgagt tgcccttgca aaagctacta tgaattatgc
     1441 tctgggagct gtcgttccaa gcgatcgaga aagaactttt ggttacagag ctcactttga
     1501 aagaatgggc tttacgtcag aactcgctgc actcgacgaa atgaggaaaa agggagcctc
     1561 aaatgacgag gtagcagagg cattccctga aaatatatta cggtctgtag cctattttgg
     1621 aaaagcggag aatgctgcct ctgagtttgc caggctttct gagggtctcg ataacgctat
     1681 agtgcgagtt gtatcgtcaa gaccaggaac agtggaggga actctggatg tgatgaatgc
     1741 ctgctctcct gtcgaagtga ggaaacatct aaaataatgc cagacatgtt gagcattgga
     1801 gaatccatga tagagctttt ctccgaagag cctatggata cagccgaaac tttcaggcga
     1861 tctgtggcag gtgacagcct taatattatt gttgccgcca gcaggttagg aactaacacc
     1921 ggatacataa ccaagctggg cgatgatcct tttgaaggtt atctgctcga tattttttct
     1981 tcggaaggaa tagatacaac ccacgttttg tctagtgacg gatttaatgc ggtccatttt
     2041 gttactgtca tgcccgacgg agacagggag ttcgtttact acagagctgg aagtgcccca
     2101 tcaactataa aaccagaaga actaaatcct gaatatatat ctggcgcaaa aattatgcat
     2161 tgcagtggaa ttgctcaagc tatttcacca agctcccggg cgacagtact cgctgccgca
     2221 caaattgccc aattgaatgg tgtacccgtc tcctacgacc caaactacag gcatcaactt
     2281 tggaatcctt ctgaagcaaa agaagctatg gaagagctaa tgccctacgt cgacatattt
     2341 ttgccaagcg cacctgctga ttctaaggta ctgttcgaga ctgaggaccc tgagaaggtc
     2401 attcaggaag caaatcgtag aggagttgaa actgttgtgg tgactcttgg ggaagatggg
     2461 gcattgatag gagctgaaaa tgaggtaatg caactggatc ctatgccagc agggcctgtc
     2521 atcgatacga ctggagcggg ggattccttc aaaggtggat ttattcatgg cttccttgaa
     2581 ggaaaatcga tcgccgagtc agctctgata ggcaacgtct gtgcaggcat aaccatcact
     2641 ggaagagggg cacttaatcc aatgccctac cgaaaagaag tatatgacaa ggttagaaat
     2701 ttaagatagc ggagatacac aaatatgaat gtacaggaaa atgtacaatt tcttattaac
     2761 agcctagatc agatatcccc atgtggcggc tgcgggatgc gatggagcac aggggattac
     2821 gagtgccctc attgtgggga agatcttgat gaaaacctca cggtctgggc ggaatcagtt
     2881 ctcaaaaatc tccccgccca gacttgagca actatctagt tggtttcaac taggaagtgg
     2941 cccaatcaac gccatttaac accaactttt gaaagccttc gttttgatgg cttttaccgt
     3001 catgtcctag taatgtcaca aatactttgc ctgccccata agacctggtc cacgctaatg
     3061 ggaaggtacc tccttccatt tccatatctt ctgcccctgc cctatttttc ccatatacac
     3121 caccttgaaa gtcagccgtg ataaacacgt cattgccatc tctataatca atgttcatat
     3181 acagctcatc atcggtgttg aatgctgaaa tacttgaagc acacggatgt gacccattca
     3241 caacatcgac ggaaaaattg ctatagggtg gatgaaaact ttgacccatt acccagccgc
     3301 caccggtgat ttctccatat tcgttccatg tatcaggaac acaacctgaa atgtggatgc
     3361 acacgaaacc attcccggaa ctaatataac cttgtaatgc gttctgttgt gcagtatcta
     3421 aagccatatc accctctcgg agggtattaa aaatcaaaac atcagactta tttaaagtac
     3481 cgtcagtcaa tgcctctgca tcttctctta cgataacagt atggcccgcc gactccaaaa
     3541 aacctttaag cactggagtt gattcctcat atggatgcct accaccgctc attaataaca
     3601 aattcatgaa atcctcctta caattttcgt ttttctgaac ttaatttacg atcaaacgat
     3661 ttttgttatc atcactaccc gcatatagtc gatttatatt gctataaatg tcaggattca
     3721 aatctcttcc atcaagagtg gggtcttctg gcgagtatct atgggcccgg aataccatat
     3781 ttgttgggtc tcccgatctt aaatcaggag agtagaaagg gtttatgtat tcccaaacaa
     3841 cgttaccgtt tttggtgacc tcaaaaattc ttccgaaagc accctcacaa attaaagtgt
     3901 tcccattggc cagtctttcg gcgctgctta tatgaaaact ataaaacgcc atagctggag
     3961 tctcagtgta ttcccattca atttcatttg ttattggatt tacctcaatt acacgagatc
     4021 tatccgcacc ctgggagtga ctaccgttat cgaatattaa tatgttccca ttttccaaaa
     4081 cggtaggatg gtgttggtga aacacatttc cagggcccca tttccaaaga aattctccag
     4141 tttctctact aattatcccc acggtgctaa tatttcgaaa gcttattaaa aaatctccat
     4201 taggcatcaa attcaagcag ttggcatggg tccactccct ccgatgctct aaagggcata
     4261 tcacatcttc ctcaaaatcc aattcctggt agatttccca ttcatttact gtcttgccat
     4321 cttcagtaat ctctcttata acatccccta atattctttt agggtcatct tctttaggat
     4381 ggcctccttt tacttgttga gaaatctcct catccagttc tttaaataaa agaacacttg
     4441 tattaccgtt aggtaacctc gcataatcat gatgcaacat aggatttttg tattcccaca
     4501 caatctctga atcccaatcc atctctataa gcgaggctga agatccgccc aagtctttca
     4561 caatttctac atctctggga ggtttggttc tagccaataa gtttccgttt ggtagaagat
     4621 aggcatagac tattccccca tcataattcc atctgtggac gactcgccca agattatcaa
     4681 tcatggtggc atggttacca ccattggtac tgaataacgt atacccattg aaggtactag
     4741 aaggtgaatt atgtataagc ccaacaggat gatgaatcga tctacccatg gaatctccct
     4801 ttttgaaatc tagagaatct ggtaacccta aaaattacgt tgttaaaggt gtatcggccc
     4861 gattagccca ttcacccatt gaaccatcgt aatttctttt gttctcatac cctaaaagtg
     4921 taagtacaaa cactccgtgc gccgcacgga ttccaccctg tcaataagtt attacttctt
     4981 tttctggggt aaccccgaca gcttttagca tatctcgaag ctcagaagga ctctttatag
     5041 tgtggtattt gtcatctgtt atgaaattca accattccaa atgaacagac ccaggaaccc
     5101 ggcccgacct cgaattacct cttgaatttg tcccatccca ttccccatcc gaccttacat
     5161 caagaaaaac aacatcggaa tcatcgatct tggaaacagc ttgatctaaa gtgcatatta
     5221 ttgtttcatc agcagacggg gtaaaggaaa cttcctttgg ggaaggaggt tctattgaca
     5281 ctggcctacc ttcatcgaac cattttttcc atccaccatc taacactttg gcatttttat
     5341 gcccgtaata gttcaatacc caccaaaacc tactagccca tagacttcca ccactgtcat
     5401 aactcactac agtagtatca tccccgatgc ccatactttc aaaaagttct ttaacaacat
     5461 ccggttccgc gaccagtggg tatgacttgg aatcttggga atacgaaggg tgcttgatgt
     5521 aatggtgcac ttttatacca accgcccctt ttatgtgtgc tctcgcataa gaatcgaaca
     5581 tatcacagtc aacgatccgg atatccgaat catctatgtg gctatttaac cattcagtat
     5641 taacaagtat tccggaattt gcgaaacttt cagtagccaa tacacccctc cattaatttg
     5701 agcattaaaa cacgggccgt attacccgaa ccatcataag ataaacgact aggaagcaac
     5761 ggtcaaaaaa ggtattacct cttttcttag gatttccaca tgaaacatat ggtcaaatac
     5821 aggattaaac atcaacatac ctgcacctga atcaatt
//
LOCUS       DASCNS010000194        14826 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001532422, whole genome shotgun sequence.
ACCESSION   DASCNS010000194 DASCNS010000000
VERSION     DASCNS010000194.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 14826)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14826)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14826
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001532422"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="QF838_06945"
     CDS             <1..422
                     /locus_tag="QF838_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60453.1"
                     /translation="DPHTTSDPDKYRNPDAIALKLRKFAALDPREASGMQKGSRMELA
                     IWEEFSSNPGLLSREAEKIISSINIPAKISDPNSKSLKVSYVKNTVNSGKEAFPSIGN
                     KLRPLYRSARKHPLKIVIGSAFGLLSTAAVLLIKKKK"
     gene            467..1591
                     /locus_tag="QF838_06950"
     CDS             467..1591
                     /locus_tag="QF838_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60454.1"
                     /translation="MKITDITVNRIRAPQGNNARSFVGRPGRLLLQVHTDEGMTGISE
                     AGRNLKVVRAYLEELIKPLLVGADPTRPRRVWELLTLGEGQQATRFPSQIVGSVDVAL
                     WDITGKAANLPIYQLLGGARRTDIPLYWSRGNGWSKSPEEMLEEIQEGFDKGYRAFKV
                     RMDWRDYRQDSDPKKDLAIFEKCREWLPDDCPLSFDANCGYSVPTAIEQGRRFEQLGI
                     AHFEEPLPQYDFPGLKQVVDALDCSVSTGEQEISSWRFRDLIELGNPDILQPDILNVG
                     GLSEMIRVYELAVVHNKVIMPHSPSVGANSMASLHLYSTVTNAIRPHEFSEEFTGPAE
                     EVSRLYKEPILPKNGVISLPEKPGLGLEVDPVALKQFIDE"
     gene            1640..3205
                     /locus_tag="QF838_06955"
     CDS             1640..3205
                     /locus_tag="QF838_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HJO60455.1"
                     /translation="MKYDTIIIGAGSAGSILATRLTEDPNKSVLLLEAGPDYPDLDSL
                     PEEVKFGYATGTEISTSDHNWQYTARGTDKAEISVPRGKVTGGSSAINGQIFLRGIPQ
                     DYDNWSYMGNDLWDYQQLLPYFNKVETDTTYQDDPGDFHGSTGPIICHRFPRDKWLPA
                     SEAFEKACLDEGFPACEDANAPGTTGVGPLPFNNLNGIRWSTAIGYLGLSRHRLNLII
                     RPEVVVKKIIFDTSDHEPKATGVEVVSGDETFILEGEEIILSAGAVVFPQILMLSGIG
                     PRDNFSSHGIEIIHDVPAVGQNLADHPMVYVTWKTKPGVKLDFLGPRIQLTLRYTAEG
                     SNLENDMIVYMMAVASDRPERGGLRNNPVGIQTNLCINLAKGKGEVKLNSDDYRDQPY
                     LDYNYLAEEEDRRRFRDGVKMLVGLENHPDMSSLIQERLTPLDSDLQSDKSLDSWLMK
                     EVTTGHHISCTNKMGPKSDPNSVVDQTGKVHGIKNLRIVDASIMPECVRANINVTVMT
                     MAERISDLIKEDS"
     gene            3264..3647
                     /locus_tag="QF838_06960"
     CDS             3264..3647
                     /locus_tag="QF838_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HJO60456.1"
                     /translation="MPSQFDKGLEMRKSVLGEEYVEESFARATDFDRPFQEFITENAW
                     GGVWTRGPLDKKTKSLITLSVLVALRAEGEIGLHTRGAINNGVTPEEIVSLLIHASAY
                     AGVPAAVSSIKIVKKTLQDMGELPS"
     gene            complement(3652..4836)
                     /locus_tag="QF838_06965"
     CDS             complement(3652..4836)
                     /locus_tag="QF838_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60457.1"
                     /translation="MDKALQGIRILDLTQYEAGPSCTQVLGWLGADVIKVEQPGKGDP
                     GRSVQLSGANKTSPYFLNFNSNKRSVTLDLKNEKHREVFSDLVTKCDVLVENFASGVL
                     ERLGYTYDTLSNINSQIIFARLKGFGTYGPYSEYKSFDPVAQAAGGAMAVTGIPGGPP
                     LKPGPNIGDSGTGLHGVVGILAAIIQRQTTGKGQVLEVSMQDAVVSLSRASMSIYSDT
                     GEPPARRGPRIPGRAGAGIYKCAPGGPDDYVYILAMSQRRNIWEDFMNAINRRDLCDS
                     EIFDDPNKNAEQIDSVIEAWTINNTKQEAMSVLGGAGIPCGAVLNARDIYNNEHLQQR
                     DMIVDIEHPDRGEFQVLGCPIKLSDSVAEIVTPPLLGQHTKEILSEVLGYTEDRIKSV
                     ID"
     gene            4939..5178
                     /locus_tag="QF838_06970"
     CDS             4939..5178
                     /locus_tag="QF838_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012675.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HJO60458.1"
                     /translation="MSSIKMYGTTWCGDCTRAKAFFDRNQIDYDWTNVDDEPEYQEYI
                     KELNNGNQRVPTIIFSDGTFLVEPTNEDLAKKLGL"
     gene            complement(5179..6288)
                     /locus_tag="QF838_06975"
     CDS             complement(5179..6288)
                     /locus_tag="QF838_06975"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HJO60459.1"
                     /translation="MHQGIYELANPNALDTPAMLTYSHIVEENINEIIRICGKPENVV
                     PHAKTHKSSDVLNIQLDRGIKSFKCATLKEAEVVAGCGASEIVIAYPMVHPRKLERFS
                     NLIKSFPGTEFRAIASTKVHLNLLSGVSKAIYGDIGVYMDLDTGMRRTGVQPGTNANE
                     FYKSIDATDGLSPIGIHVFDGETLYIPSFEKRSLIVKRNIGFMEEIWSAANTNGIQVK
                     DNLAGGSWSFQHYADHPNIRPTPGTWIYWDTRNAEMEELGFRIASLILGQIVDEDAEM
                     DTVTIDIGSKSCSSDQPLQHRFKLVHYPDTELVIQNEEHAVVKLNGASLNVGDFVIAA
                     PGHACTLTVKFPYTNVVSKDGQIVGRYMHDARDRI"
     gene            complement(6326..6604)
                     /locus_tag="QF838_06980"
     CDS             complement(6326..6604)
                     /locus_tag="QF838_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HJO60460.1"
                     /translation="MLFLVVSSPNPERPSEVKEQRRKYWPWAQDKLDKGLAISFYART
                     GRGAVATFDVDSNEVLHCLLNEWSEIVPAKFDIYPLLDSESIKSFLGS"
     gene            6690..7793
                     /locus_tag="QF838_06985"
     CDS             6690..7793
                     /locus_tag="QF838_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJO60461.1"
                     /translation="MNSTNNEDLNPQEQSVFFYGDDAPFYNVEFINHLNHISESKKIS
                     EDNYSIGGEVEKLETTMAQKLGKEASVFFPTGTLANHVAIRQLCLSNKRAIVPEQSHI
                     YQDSGDAVQQLSGINLIPLGPNKPYFGLDELKGALNTSISGRVTTPVGAVVIETPVRR
                     CYGRVMPYEAMKDVTGYCHQQGIPTHLDGARLLIASAVTKISLEAYSNLFDTVYISMW
                     KYFGAPYGAVLAGSKEFCENLYHVRRMFGGSLPSASLAAALALNGLETFQTDAELANE
                     KSAELLESLNGLPNINVKRYQDGSNIIPVEIDQNVDLPTLYERLRSQNVFINSDTKSE
                     NILHLTMNLSILRKPNDELLRIFAKAIEDAKSI"
     gene            complement(7800..8723)
                     /locus_tag="QF838_06990"
     CDS             complement(7800..8723)
                     /locus_tag="QF838_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HJO60462.1"
                     /translation="MKLAFFNNFTLGVVKGDKIVDISKAVPVDEHNHAQGLLNRIIES
                     FDDYRPGIEKLVAAEEGVDIASVTFNSPVPKPHNIVCMAVNYMEDGTRDEPAPINAFP
                     KSPNAVLPDGGTIVLPDVPATIFEPEAELALVIGKKATKIKAEEAYDYIFGYMNFIDM
                     SARGLTSGGGVFYQMKSRDTFAPMGPYLVTADEIKNPQNLQINLHVNGDLKQNFNTDD
                     MAHKIPRALEWITSIHTLEPGDVLATGTNHRGLSPIMHGDTVTLEAEGLGLLTLKVRD
                     DLKREWERATRLEREEQGHSDTAPQIKGKYS"
     gene            9075..10352
                     /locus_tag="QF838_06995"
     CDS             9075..10352
                     /locus_tag="QF838_06995"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60463.1"
                     /translation="MISAISQKFLGPAMAYPSYRAYWFGTLASVIGFQMLNFSQFWII
                     HDLTESPLYLGYVGLANALPAIVLNIFGGVVADRLDRKKLIGITQTINGLIILVLAGL
                     TFAGVVQAWHVIALAFLTGAINAFDQPARVAIYPSLIDKSVMMNAVALNSAIWTGTRI
                     IAPAIAGVVISLTGTATSFLISALGFFIMAAVVLSITVPKDESEAQPASKGNDLLEGV
                     RFIWKNNVFLILICITFFNSFFGMAYIPMMPVFAVDILKVGADGQGVLMGLGGIGALS
                     ATMIIGRTGNFKQKGLLIIFGSVFFGLSIAGFALSSMQYGNYYVALVLLFIVGVSSSC
                     YMVSIMSSLQIMVPNHMRGRVMGFYGMTWSIMPLGGMYAGALSRYIGEDITGVPIAVA
                     IGGILIAVFAIGPFFMNRNVRNLGTILQTHEAN"
     gene            complement(10393..11640)
                     /locus_tag="QF838_07000"
     CDS             complement(10393..11640)
                     /locus_tag="QF838_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60464.1"
                     /translation="MFSRLYYGWWIVLGAILCNFAFLSVSQSAIGVFTLPMVDDLGWK
                     VWQYTLGPSIAIGTGAFSSIVVGSILDKRGPKPLIFTGASMSAVCLVLLGIQSSLMVY
                     LFVYLIAGFVGWNLFSPFVVNSAVNKWFVRKRGWALALGSSGISLGSLITPLFLTGVV
                     DTSGWRTGYFSLGVFILILIIPVSFFMRRTPEDHGLLPDGIDDYDDPSFLDIPESEFR
                     PLNRSEAINTSSFWFLAFGFTLIAAGLACVLIHAIPFSQESGFARTVGAIGISVNGLA
                     NLSSKPVWGFAMQRFHPKFLVMVAYSISSIGVSLMLLSGPISLIPMLLAGFFLYGFGF
                     GGTIPLSESLWARYFGRAHIGSIRGITQPIRILGTAVAPVLVGLLFDITDTYRPGFVL
                     VIGALLLGAILVFLSREPQMTEP"
     gene            complement(11780..12097)
                     /locus_tag="QF838_07005"
     CDS             complement(11780..12097)
                     /locus_tag="QF838_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60465.1"
                     /translation="MKETFGNILSIFRELRGVWGVDVEVGVRGIGEGVILAGGTNTNG
                     GVAVDAFLVSTSRPLGWVTNLVGEAKGSCGPSSETEEQENSISNTSIKKPGFFIQSCL
                     QNA"
     gene            12102..13130
                     /locus_tag="QF838_07010"
     CDS             12102..13130
                     /locus_tag="QF838_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HJO60466.1"
                     /translation="MVDLVFSGVGTPQAFLVLQPGRIVVFNFNERDPQPQEFLDIRGR
                     VSDKGNEEGLLGMALSPDFSGDGHFYLYYSAANPRRSVLSRFTIRPGDISSGDPSSER
                     VIFEVPQPYSNHNGGQIRFGPQGLLYIGLGDGGKKGDPIGNGQNLTTLLGSIIRIDVR
                     TLDSKGTYTVPSDNPFTGSSNAKGEIWAYGLRNPWRFSFDSQTGSLWVGDVGQNDFEE
                     INLVTGGSNYGWNIMEGSHCYADSDEACDQSGLELPVAEYGREEGCSVTGGYVYRGSR
                     LPELAGAYVYGDYCTGKIWALRHDGNEITEHLQIADSSLNISSFAQSPAGEIFILSFD
                     EKIYRFTR"
     gene            complement(13242..14084)
                     /locus_tag="QF838_07015"
     CDS             complement(13242..14084)
                     /locus_tag="QF838_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60467.1"
                     /translation="MLIFIRGKLCSVTFVVMLIILISIIQTGCLVLDKYFVYFPNHEL
                     PMTPSDVGLNYEDVYLTSADGIELHGWFLPGESEIALVWFHGNAGNIGDRLDYIKLMH
                     TYVGASIFIFDYRGYGLSQGSPSESGLYLDAEAAVDFLIDRVGSEEGTQNVFFFGRSL
                     GSAVALKMASIYDVGGVIIEAPFTSTVALAKIHYPYLPEFLVNLLIRDRYASGSMAGS
                     LGSPLMVIHGTNDSVVPIEMGEKIYDLANVNKSFYAVVGADHNDTYIIGGENYFRKIL
                     EFVE"
     gene            14248..14460
                     /locus_tag="QF838_07020"
     CDS             14248..14460
                     /locus_tag="QF838_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60468.1"
                     /translation="MFITYISWLPSRVEWKAILAPSGVSVGVGVAADAVTDCCVPPHP
                     IVQSIKIKHGNINGLLNFIPSPPWQF"
     gene            14616..>14826
                     /locus_tag="QF838_07025"
     CDS             14616..>14826
                     /locus_tag="QF838_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60469.1"
                     /translation="MKHLTYVIVAGLISLLIACGVNTENVDATDEVSLVKEKAAIADT
                     ATSLPPTSTPVTKPTAIPPPTQTVRV"
BASE COUNT         4465 a         3260 c         3108 g         3993 t
ORIGIN      
        1 cagaccctca tactacatct gatcctgata agtatagaaa tcctgatgcc attgccctaa
       61 agctccgtaa atttgcagcc ttggatccca gggaagcctc tggcatgcag aagggaagca
      121 gaatggaatt ggcaatttgg gaagaatttt cctctaatcc cggcctttta tctcgagagg
      181 cggagaaaat catcagttcc attaacattc cagccaagat ttctgatcca aattcaaaaa
      241 gcttgaaagt tagttatgta aaaaatactg taaattctgg aaaagaagct tttccatcta
      301 taggaaacaa actccgacca ttgtaccgat cagccagaaa gcatcctcta aagatagtta
      361 tcggttcggc cttcggccta ctttccactg cagcagttct attgatcaaa aagaagaaat
      421 aatcataatt caataagaaa ttcacaaaaa gaagcgggag gctaatttga aaatcaccga
      481 tatcactgta aatcgtatcc gagctcctca aggaaacaac gcaagaagct ttgtgggcag
      541 gccaggacgt ctccttttgc aagtacacac cgacgaaggt atgactggta tttccgaagc
      601 aggaagaaat ttgaaagtgg taagagctta tttagaggaa ttgataaaac ctctactggt
      661 cggagctgat cccacccggc ctcgaagggt atgggaatta ttaaccttag gtgaaggaca
      721 gcaagcaact cggttccctt cacaaattgt cggttcggtt gacgtggccc tttgggatat
      781 aacgggtaag gcagctaatt taccaatata ccaacttcta ggtggagcca gaagaactga
      841 cattcctctt tattggagca gaggcaatgg gtggagcaaa agccctgaag aaatgctaga
      901 agagatccaa gaggggtttg acaagggcta tagagctttt aaggtaagaa tggattggag
      961 agattataga caagattcag accccaaaaa agacttggcc atttttgaaa aatgccggga
     1021 atggttacca gatgattgcc cgttaagctt tgatgccaat tgtgggtatt cagtgcccac
     1081 agcaattgaa cagggaagaa gattcgaaca attaggaata gcgcactttg aagaaccact
     1141 gccacaatat gatttcccag gcttaaagca agttgttgat gctcttgatt gttcagtttc
     1201 tacaggggag caggaaatat catcgtggag atttcgagac cttatagagc ttggcaatcc
     1261 ggatatactc caaccagata tattaaacgt tgggggttta tcagaaatga tacgcgtcta
     1321 tgagttagca gtggttcaca ataaagtcat tatgcctcac agtccaagcg ttggagcaaa
     1381 ttcaatggca agcctgcatc tctattctac cgttaccaat gcaatcagac cccacgaatt
     1441 ttcagaggag ttcacaggcc cagccgagga ggtatctagg ctatacaagg aaccaatact
     1501 gccgaaaaat ggggttattt ccctgccaga aaaaccgggt ctgggcttgg aagttgatcc
     1561 cgttgcccta aagcagttta tagacgaata aactttatcg ttgtaagtaa tccaatttga
     1621 cctaaggaat agtcatagca tgaaatacga cacaatcatt atcggggcag gttccgccgg
     1681 atccatctta gctacccgct tgaccgaaga ccccaataag tcagtgcttt tgcttgaagc
     1741 cggacctgac tacccagatc tggatagcct ccccgaagag gttaaatttg gctacgctac
     1801 tgggaccgaa atatctacca gtgaccacaa ttggcaatac actgcaagag gaactgacaa
     1861 agctgagata tcggtaccaa gaggtaaagt caccgggggt tccagcgcaa tcaacggcca
     1921 aatatttctt aggggaatcc ctcaggacta tgacaactgg tcttatatgg gcaatgattt
     1981 atgggattac cagcaactgt tgccatattt caacaaagtg gaaaccgata ccacctacca
     2041 agatgatcca ggagattttc acgggtctac aggaccaata atttgccata ggtttccaag
     2101 agacaaatgg ctcccggcaa gtgaggcctt tgaaaaggct tgtttggatg aagggttccc
     2161 tgcctgtgaa gatgccaatg caccaggaac aacaggtgtt gggccactcc cttttaacaa
     2221 tctaaatgga ataaggtggt ccaccgccat aggttattta gggttatcaa gacatagatt
     2281 aaatctaata atacgaccgg aagtagtggt aaagaaaatc atatttgaca cctcagacca
     2341 cgaacctaag gccacaggag tggaagtagt gtcaggagac gagactttca ttctagaagg
     2401 ggaagaaata atactcagtg ctggcgcggt ggtttttcct caaatactca tgttatccgg
     2461 aatcgggcca agagataatt ttagtagcca cggaattgaa ataattcatg atgtaccggc
     2521 agtaggccag aatctagctg atcatccaat ggtatacgtc acatggaaaa caaagcctgg
     2581 tgtgaagcta gattttttgg ggccaaggat acagctgact cttcggtata cagccgaagg
     2641 ctcaaatctt gaaaacgata tgattgttta catgatggct gtcgcaagcg acagaccaga
     2701 aagaggtgga ttgagaaaca atcccgtagg gatccaaacc aatctatgca ttaatttggc
     2761 taaaggaaaa ggggaagtaa agttaaattc agatgattac agagaccagc catacctaga
     2821 ctacaattac ctagcagagg aggaagatcg gagacggttc cgcgatgggg tgaaaatgtt
     2881 ggttggttta gaaaatcatc ctgatatgtc atctcttata caggaacgat taactccgtt
     2941 agactctgac ttacaatctg ataaatcgtt agactcctgg ttaatgaagg aagtcactac
     3001 agggcaccat atctcctgca ctaacaaaat ggggcctaaa tctgatccca attcagtggt
     3061 tgaccaaacg ggaaaagtcc acggcattaa aaatttgagg atcgtcgacg cctcgatcat
     3121 gccagagtgc gttagagcaa acattaacgt cactgttatg acaatggcag aaagaatatc
     3181 ggacttgatc aaagaagata gctaatataa aactacttta gttaacataa tctaatcgga
     3241 cataaataac ggagccattg aaaatgccta gccaatttga caaaggctta gaaatgagaa
     3301 aatcggtact tggtgaagag tatgtggagg aatcgtttgc cagagcaacg gattttgata
     3361 gacctttcca agaattcata actgagaacg catggggagg agtgtggacg agaggacctc
     3421 tcgataagaa gacaaaaagt cttataactc tctcagtttt agtcgcgctc cgtgctgaag
     3481 gtgaaatagg attgcacaca agaggtgcaa tcaacaacgg cgtcacaccc gaggagatag
     3541 tcagcttgtt gatacatgcc tcggcttatg caggtgtgcc agcggccgtg tcatccatca
     3601 aaatagtaaa aaagactctt caagatatgg gcgaactacc ctcataaatt gttagtcaat
     3661 taccgactta attcgatcct cggtataacc taaaacttca gaaagaattt cttttgtgtg
     3721 ctgccctaat aaaggcggtg taactatttc ggcaactgaa tctgaaagct tgataggaca
     3781 tcctagtact tggaattctc ctctgtccgg atgttctata tcgactatca tatcccgttg
     3841 ctgtaggtgt tcgttgttgt agatgtctcg ggcgtttaaa actgccccac agggaattcc
     3901 tgcgcccccc aagactgaca tagcctcttg tttagtatta tttattgtcc aagcttcaat
     3961 caccgaatct atttgttctg catttttatt agggtcatca aaaatttcag aatcgcatag
     4021 atctcttcta tttatcgcat tcatgaaatc ttcccaaata tttctccgtt gagacatggc
     4081 caagatataa acgtaatcgt ctggtccgcc aggggcgcat ttgtatatgc cagcgcctgc
     4141 acggccggga attctggggc ctcttcttgc gggaggctca cccgtatcac tgtatatact
     4201 catcgaagct ctggacaaac taactacggc atcttgcatt gacacctcta acacctggcc
     4261 ttttcctgta gtctgtcttt gtatgatggc agctaatatt ccaaccacgc catgcaatcc
     4321 agtgccacta tctccaatgt tcggacctgg tttgagtgga ggcccacccg gtatgccagt
     4381 tactgccatg gcccccccgg ctgcttgagc gaccggatcg aagcttttat actcactgta
     4441 gggcccatat gtcccaaaac cttttaatct tgcgaatatt atttgtgaat ttatattgga
     4501 caacgtgtca taagtatacc ctaatctctc caaaaccccg gaagcgaaat tttcaaccaa
     4561 aacatcacat ttagttacta gatcgctaaa tacttccctg tgcttctcgt tctttaaatc
     4621 caaagttacg cttcgcttat tactgttgaa atttaaaaaa tacggcgacg ttttgttagc
     4681 acccgaaagc tgtacggatc gacccgggtc acctttgccc ggctgttcca ccttaatgac
     4741 atcagcacct aaccatccca atacttgagt acaagaaggt ccggcttcgt attgagttaa
     4801 atccaaaatc cttattcctt gcaaagcttt atccatagct aaacacctcc ctgtaactaa
     4861 gtggtcaagt ggcagtatat acctgttcaa agactattag aacgtctaga atatgggctt
     4921 gattaagagg agttacaaat gagttcaatc aaaatgtatg gcacaacttg gtgtggagat
     4981 tgcactaggg ccaaagcgtt ttttgaccgt aaccaaatag attacgactg gactaatgtt
     5041 gacgatgagc cggagtatca ggaatatatt aaggaattaa ataatggcaa ccaacgagtt
     5101 cccactatta ttttttccga cgggacgttc ctagttgaac caacaaatga ggacttagca
     5161 aagaagttgg gactttaatt aaatcctatc tcttgcatca tgcatgtacc tacccactat
     5221 ctgaccatct tttgacacta catttgtata tggaaattta accgttaatg tacaggcatg
     5281 tccgggagcc gcaatcacaa aatcacccac attcagtgaa gctccattca acttaacaac
     5341 cgcatgttcc tcattttgaa taactagttc agtatccggg taatgaacta gtttaaatct
     5401 atgttggagc ggttgatctg aagaacagga tttagatcct atatcaatag tgactgtatc
     5461 catctccgca tcttcatcaa ctatctgacc caatattaag ctggctatgc gaaatcctaa
     5521 ttcttccatt tcggcatttc gagtgtccca gtaaatccaa gttccgggtg ttggtcgaat
     5581 attgggatgg tctgcatagt gttgaaaaga ccatgatccc cctgcaagat tatctttaac
     5641 ttgtattcca ttagtattag cagcagacca aatttcctcc ataaacccta tgttcctttt
     5701 aacaataagg ctcctttttt cgaagcttgg aatatataag gtctcaccat caaaaacatg
     5761 tataccaata gggcttaacc catcggtagc atcaatgctt ttgtagaatt cattagcgtt
     5821 tgtaccaggt tgaactcccg tacggcgcat acctgtatca aggtccatat aaacgccgat
     5881 atccccatat attgctttgg aaactccaga taaaagattc aaatgcactt tagtgctggc
     5941 aattgcccta aattctgtgc ctggaaacga tttaattagg ttggaaaacc tttctaattt
     6001 ccttggatgc accatgggat atgcgatgac gatttcgctt gcaccacacc ctgctactac
     6061 ttcagcttct ttcagcgttg cacatttaaa agattttatt cccctatcca attgaatatt
     6121 caaaacatca gaagatttat gtgtcttcgc atgtggtaca acattttctg gcttaccaca
     6181 aatgcgaata atttcattga tattttcttc cacaatatgg gaatatgtga gcatagcggg
     6241 tgtatctaaa gcgtttggat tagccagttc atatatgccc tgatgcattg ttcctcctgt
     6301 tgaatcatta actcatatat ttcggtcagc tacccaaaaa agactttatt gactccgaat
     6361 ccagtagcgg atagatatca aatttggctg gaacaatttc cgaccattcg ttaagaaggc
     6421 agtgaagcac ctcgttggaa tcaacatcaa atgtggccac agcacccctt ccggtgcgag
     6481 cataaaaact tatcgccaaa cctttgtcta atttatcctg agcccacggc caatattttc
     6541 gcctttgttc ttttacttca gaaggtcttt caggatttgg tgaactaact actagaaata
     6601 acatacttgc tcccgtggcg gaaattatat actcagaacg ctgacaaatt gctaaggcaa
     6661 ataaaatata taaaaaagga atactcagta tgaattccac taacaacgag gatctcaatc
     6721 ctcaagaaca atccgtattc ttttatggtg atgatgctcc attttacaac gttgaattca
     6781 ttaaccatct taaccatatt tcagaatcta aaaaaatatc ggaagataat tattctattg
     6841 gcggtgaggt ggaaaaatta gaaacaacta tggcgcaaaa actgggaaag gaagcttctg
     6901 tcttttttcc aaccggaact ttagccaacc acgtggcaat taggcaatta tgcttatcca
     6961 acaaaagagc cattgttcca gagcaaagcc atatttacca agacagtgga gatgcggtcc
     7021 agcaacttag tggaataaat ttgattcctc ttggcccaaa caaaccatat ttcggtttgg
     7081 atgagctaaa aggagcatta aatacatcaa ttagtggaag agtgactacc cctgtaggtg
     7141 cagtggttat tgaaaccccc gttaggagat gttacggacg ggtgatgcca tatgaggcga
     7201 tgaaagatgt aaccggttac tgtcaccaac agggaatccc gactcatctg gatggagctc
     7261 gccttttgat agcgagtgct gtaactaaaa ttagtttgga agcctatagc aacctgttcg
     7321 atactgttta tatatcaatg tggaaatatt tcggagcccc ttacggtgcg gtactggcag
     7381 gatcaaagga attttgtgag aacctatacc atgtgaggag aatgttcggc ggtagtttgc
     7441 cgtctgcctc tttagcggcg gcactggctc taaacggcct tgagactttt caaaccgatg
     7501 cagaattggc aaacgaaaaa tcggccgaat tactggaatc tctcaatggg ttaccaaata
     7561 ttaatgtgaa gcgataccag gacgggtcta acatcatccc tgttgaaatt gaccaaaatg
     7621 tagacctgcc cacattatac gaaaggcttc gttctcaaaa tgtcttcatc aacagcgata
     7681 caaagtcaga aaacattcta catctaacca tgaatctttc catactgagg aagccaaacg
     7741 atgaacttct cagaatattt gcaaaggcta ttgaagatgc taagtcgatt taaggtcgct
     7801 tatgaatact tacctttaat ctgtggcgct gtgtcactgt gaccttgctc ttctctttct
     7861 aatcgagttg ccctctccca ctcccttttt aaatcgtctc gaacttttaa ggtcagtaaa
     7921 cccaatcctt ctgcctcaag agttactgtg tcgccgtgca taatagggct taaacctctg
     7981 tggttagtgc ctgtagccaa cacatcccct ggctctaaag tatgtattga agttatccac
     8041 tccaaagctc gaggtatttt gtgagccata tcatcagtat taaaattctg tttcagatcc
     8101 ccattcacat gtagattgat ttgtaaattt tgcggatttt taatttcgtc agcagtaact
     8161 aaataagggc ccataggagc gaaagtatct cttgatttca tttggtaaaa cacgccacct
     8221 ccgctggtta accctctagc cgacatgtct ataaaattca tgtatccaaa gatatagtca
     8281 taagcctctt cagctttaat tttggtggcc tttttcccta ttaccaaagc caactcagct
     8341 tcaggttcaa atatcgtggc tggtacatca ggtaaaacaa tagtcccacc gtccggcagc
     8401 acagcgttgg gtgattttgg aaaagcattg ataggagcag gttcatccct ggtaccatct
     8461 tccatgtaat taaccgccat gcaaacaatg ttgtgtggct ttgggacagg cgaattaaaa
     8521 gttacggaag caatgtccac tccctcttca gcagcgacca gtttctcaat tccaggtctg
     8581 taatcatcga aggattctat aatcctattt agtaatcctt gggcatgatt atgctcatcc
     8641 acaggaaccg ctttggatat atcgacaatt ttgtcccctt taacgactcc caaagtaaaa
     8701 ttattaaaaa acgctagttt catagctatt acactccttc cgaaaaaacc cgtttccaaa
     8761 acactaatcg ttagcatatc attaagctaa atgataagtg tcatgcatta aaacattagg
     8821 aggggaacta tgccttcaat agcaggataa ggttaagtat tagggaacaa gcaaaaatca
     8881 gagccattgg gtcctgggtc actcaagaat cattgcagat agtgacaaaa tttttccagt
     8941 atgcaggcgg tgccgccctt aatagaaaca atctcctgca gcagtgcttt agagacatgt
     9001 atgccgcctc ccagcattac atggtcagca ctacgcattt tcaatcttat ggaagggagt
     9061 acttcagcgc ataaatgata tcagcaattt ctcaaaaatt tcttggtcca gcaatggcct
     9121 acccctctta ccgggcttac tggttcggca cattggcatc agttatcggg tttcagatgt
     9181 tgaacttcag tcaattctgg atcatccatg atttaactga atcacctcta tatctgggtt
     9241 atgttggctt ggctaacgca cttcccgcca tagtgttgaa tatctttggt ggagtcgttg
     9301 cagacaggct agaccgaaaa aaattaatag gcataaccca aacaattaac ggtctaataa
     9361 tattggttct agcggggtta actttcgcag gggttgtaca ggcatggcac gtcatagctt
     9421 tggccttcct taccggagct atcaatgctt ttgaccagcc agcgagagta gctatatacc
     9481 ccagcctaat cgataaatca gtaatgatga acgctgtggc actaaattca gcaatatgga
     9541 caggaaccag aataatagcc ccggctatag caggagtagt aatttcccta actggaacgg
     9601 ctacgtcttt tctaatttcc gccctagggt tttttataat ggcagcagta gtactctcaa
     9661 ttacagtacc aaaagatgaa tctgaagctc aaccggcatc gaaaggaaat gatttattgg
     9721 aaggggttcg tttcatatgg aaaaacaatg tgtttttgat attaatttgt attacattct
     9781 ttaacagttt ctttggcatg gcatatatac ccatgatgcc tgtttttgcc gtagatatac
     9841 tcaaggtagg agcagatggt cagggagttt tgatgggtct aggcggaatt ggcgcactat
     9901 cagccaccat gattattgga agaaccggaa atttcaaaca aaaaggactg ttaataatat
     9961 ttggatccgt attttttggt ctttcaatag ctggttttgc cctctcttca atgcaatatg
    10021 gaaattatta cgtcgcactc gttcttcttt ttatcgtcgg ggtaagtagt tcgtgctaca
    10081 tggtatcgat aatgagttcg ctacaaataa tggttccaaa ccacatgagg ggaagagtta
    10141 tgggattcta cgggatgaca tggagcataa tgcctctagg tggaatgtat gctggggcgc
    10201 tatcaagata tattggggaa gacatcactg gagttcccat tgcagtagca ataggtggga
    10261 ttctcatcgc cgttttcgct atcgggcctt tctttatgaa caggaatgtg cgaaaccttg
    10321 gaactatatt gcaaactcat gaagcgaatt aaataatact tggttgttaa aacaatacgg
    10381 ctgataacct acttacggtt cagtcatttg gggctctctt gacaagaaaa ccagaatggc
    10441 ccccagaaga agtgctccga tcaccaatac aaaccctggt ctgtaggtat cggtgatatc
    10501 aaacaatagc ccaaccaata caggggccac agctgtacca agtattctga ttggttgtgt
    10561 tatacctctt atggatccta tatgtgcccg accgaaatat cgtgcccaca aagattcact
    10621 tagaggaatg gttcccccaa atccaaatcc atataggaaa aaccctgcca ataacattgg
    10681 aatcagtgaa attggacccg ataataacat taaagacaca ccgattgagg atatagaata
    10741 ggctaccatc acaagaaatt tcgggtgaaa tctttgcatt gcaaaccccc acaccggctt
    10801 tgatgacaga ttggcaaggc cgttgactga aataccaatt gcgccgacag ttcgggcaaa
    10861 ccctgactcc tgagaaaagg gaattgcatg tatcagcaca caagcaagcc ctgccgctat
    10921 caaagtaaac ccaaaggcta ggaaccaaaa actactggta ttgattgctt ctgaacgatt
    10981 gagaggtcgg aattcagatt caggtatatc caagaaacta ggatcatcat agtcgtcaat
    11041 cccgtcaggt aaaaggccat gatcctctgg ggttcgacgc ataaagaaac ttacagggat
    11101 aatcagaata agtataaaaa caccaaggga aaaatacccg gttcgccaac cagaggtgtc
    11161 tacaactcca gtcaaaaaca gaggggttat taagctaccg agagatattc ccgacgaccc
    11221 tagcgccagt gcccatcccc tcttcctgac aaaccactta ttaacagcag aattcacaac
    11281 aaatggacta aataaattcc atccaacgaa tccagcaatt aaatatacga ataaataaac
    11341 cattaaactg gactggatcc caagtaggac caggcagaca gcactcatag aagccccggt
    11401 gaatatcagc ggtttgggtc ctctcttatc aagaatactt ccgactacta tgctcgagaa
    11461 cgctcccgta ccaattgcaa tggagggtcc caacgtatat tgccaaactt tccagcctag
    11521 atcgtccacc atgggtagag tgaatacgcc aatggcagat tggcttacac tcaggaatgc
    11581 aaaattacaa aggatagctc ctaaaactat ccaccagccg taatagagac gtgagaacat
    11641 ctaaacatac taatgcattt gaatcggaga atagaataga ttttaagttg ataaaactgg
    11701 agtttattca tggcatacca accagtcgtt aacaatgcta gagttgttga gcctcagtcg
    11761 ctaaatctca acgagaaatt tatgcattct gcaaacagct ctgaatgaag aatccgggct
    11821 ttttaatact ggtattacta atactgtttt cctgttcctc tgtttctgag gatggtccac
    11881 aggaaccttt cgcttcgcca actaaattag tgacccagcc taagggtctt gatgtagaaa
    11941 ccaagaatgc atcaacagcg acaccaccat ttgtattcgt ccccccagct aatattaccc
    12001 cctctccgat accccttacc ccaacttcca catcgactcc ccacacacct cttagttccc
    12061 tgaatatcga gagaatgttt ccgaaggtct ctttcaagag aatggttgac ctagtattct
    12121 cgggcgttgg taccccgcag gcgttcctcg ttttgcagcc aggtagaatc gtggtattta
    12181 acttcaatga aagagatccg caacctcaag aattcttaga catccggggc agggtaagtg
    12241 ataaaggcaa cgaggaaggt cttctgggaa tggccttatc tccagatttt tcgggcgacg
    12301 ggcatttcta cctctactat tccgccgcga acccaagacg ttctgtactt tcccgattta
    12361 ccatcagacc aggcgatatt agcagcggtg atccgagcag tgagcgagtg atatttgagg
    12421 tacctcaacc ttattccaat cacaatggag gacagattcg ctttggccca caaggcctgt
    12481 tatatatcgg tcttggcgac gggggtaaaa aaggggaccc tatcggcaat ggacagaacc
    12541 tgacaacgct gttgggttca attattcgca tcgacgttag gacgttagac tcgaaaggta
    12601 catacaccgt cccttccgac aatcctttta caggttcctc caacgcaaaa ggcgagattt
    12661 gggcgtatgg gctacgaaat ccatggcgat tcagcttcga ttctcagaca ggaagcttat
    12721 gggttgggga cgttggacag aacgattttg aggaaatcaa cctggtgaca ggggggagca
    12781 attacggatg gaacataatg gagggatctc attgctatgc tgattcggat gaagcatgcg
    12841 accaatcagg gttagagctt cctgttgcgg agtacggccg tgaggaaggt tgttccgtga
    12901 cgggcggtta cgtttataga ggttcgagat taccagaatt agcaggggcc tatgtatatg
    12961 gagactattg cactggaaaa atatgggctc taaggcatga tggtaacgag attaccgagc
    13021 acctgcagat cgccgattca tccttgaaca tatcgtcgtt cgcccaatct cctgctggag
    13081 aaatattcat actttccttt gacgagaaaa tctaccggtt tacccgttag tctgatccaa
    13141 atattttctc tcggcgaggc tccgattatt gattctgact cacataccgg tttaggtagc
    13201 acctatctat ggtgtcaaag accgacgaaa tatgatgtca ctcattctac aaactccagt
    13261 atttttctga aataattttc tccaccaatg atgtatgtat cgttgtggtc cgcccctaca
    13321 acagcgtaaa aagacttgtt aacattagct aaatcataaa tcttttctcc catctcaatt
    13381 ggaacaaccg agtcatttgt tccatgaata accatgagtg gagagccaag actgccggcc
    13441 attgaaccag aagcatatct gtcacgaatc agtagattga ccaaaaattc aggaagataa
    13501 gggtagtgaa tttttgccaa agccactgta gaggtgaatg gagcctctat tatgactccg
    13561 ccaacgtcat atattgatgc cattttaaga gcaactgcgc tacctaagga tcgcccgaaa
    13621 aaaaacacat tctgagtgcc ttcttcggat cctactcgat ctatcaaaaa atccactgca
    13681 gcttcagcat ccaaatacaa ccctgattcc gaaggtgatc cctgacttaa tccataacct
    13741 ctatagtcaa aaataaagat actggctccc acataagtat gcatcagttt tatgtaatcc
    13801 aaccggtcac ctatatttcc agcatttcca tgaaaccaga ccagcgcaat ctcactctcg
    13861 ccaggtagga accatccgtg tagttcaatt ccatctgcag aagttaagta aacatcctca
    13921 taatttaggc caacatccga tggggtcata ggcagttcat ggtttggaaa atacacaaaa
    13981 tatttatcta acaccaggca cccggtttga ataatcgaaa tgagaataat taacatcaca
    14041 acaaaggtga cagaacacaa tttaccccta ataaaaatca acataaagaa tgtccgaaat
    14101 caaataaacc taaacatatt aaagtagtct aattagttta attcactcat acgcgggagc
    14161 gggcgggcgc atctttaatc cctcttattt gatcacagca tagctgtaac attgataggt
    14221 gtgagattgg tctgactgga gccgtcgatg ttcatcacgt atatctcatg gttgccatcg
    14281 cgggtggaat ggaaagcgat ccttgcacca tcgggcgtca gcgtgggagt tggcgtggca
    14341 gcggacgcag taacggactg ctgtgtgccg ccacatccga tagtccaaag cataaaaatc
    14401 aagcacggaa acattaatgg tttgctgaat ttcataccat ctcctccttg gcaattctag
    14461 tctatcgtgc aggattaccg aatcataacg tttcatggct aagaataggc cacttctaaa
    14521 gaatcctcat aaaatcctcc tataggcaag attgtaaatt aagaccatct gctgcaacct
    14581 tattaccata gagttaaggg gataatgcgg gaaatatgaa gcatctaaca tatgtaatcg
    14641 ttgcaggatt gatatcccta ctaatagctt gtggcgtaaa tactgaaaat gttgatgcta
    14701 ctgatgaggt gagtcttgta aaagagaaag ctgcaattgc ggacacagca acatctttac
    14761 cgccaacatc tactcctgta actaagccca cggcaatccc gccaccaacc caaacagtta
    14821 gagtta
//
LOCUS       DASCNS010000195         1569 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001535737, whole genome shotgun sequence.
ACCESSION   DASCNS010000195 DASCNS010000000
VERSION     DASCNS010000195.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001535737"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="QF838_07030"
     CDS             <1..1344
                     /locus_tag="QF838_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60470.1"
                     /translation="DQVLGDDDDVADAPSAFEKSAEEAGRYQAAGIFHNCLVDGGVEV
                     KETTREEMKHCRQGVIDELDSLGTKTVKIENGKVISEPNYVALLDLDDVKEVVDTFMH
                     CAQSADAVRIIGPAVEGCKMEAKEMHHDAAGNGSSFTDHFANKIAGDNADARRTRKFI
                     DVKAEAIGDPDFQDKVNKVMYQPGRSKDEVRDSFKSLLNEAKEDLHCDPQGLRAKRCD
                     DIKFPEVNYTPEMTHNDTLGRNIPVAHQYENRINDRDDQYQGGDGQNQGLPPGITMAQ
                     FNDVFGGGQPRMVVKERVDQCVSDGNPMNECLRDVKNDMREEFKSHENFDHNKYSDRK
                     ELDNLNTNAVRQNTGNPQGGDGHTMHTNQFGGRGDGVGKRGAQDFNQCMTDNTSTGPQ
                     YNSDGTFASSPSHGDAADNCMGQASNTMRDSYGNKDMGHHAGFPQNPQSGMTTQQ"
BASE COUNT          509 a          309 c          390 g          361 t
ORIGIN      
        1 gatcaagttc ttggtgacga cgacgatgta gctgatgccc cttcagcttt tgagaaaagc
       61 gcagaagaag ccggtcgata ccaagcggca ggcatatttc ataactgcct agtagatggg
      121 ggggttgagg ttaaagaaac cactagagag gaaatgaaac actgtcgaca gggtgtgata
      181 gacgaactgg attcactagg tacaaagaca gtcaaaattg agaacggtaa ggtaatttca
      241 gagccaaact acgtggcttt gctagacctt gatgacgtca aagaagttgt agacacattt
      301 atgcattgtg cccagtcggc cgatgccgta agaatcatag gcccagctgt ggaaggctgt
      361 aaaatggaag ccaaagaaat gcaccatgac gcagccggta acggctcttc gtttacggat
      421 cattttgcaa ataagattgc cggtgataat gccgatgcaa gaagaactcg caaattcatc
      481 gatgtgaagg cagaggcgat tggtgaccca gattttcaag ataaagtaaa taaggtaatg
      541 taccagcctg gtcgttctaa agacgaggtt agggatagtt ttaaaagtct ccttaatgag
      601 gcaaaagagg atttacattg tgacccacaa gggctgaggg caaagcgatg tgacgacatt
      661 aaattccctg aagtgaacta tacacctgaa atgactcata atgacaccct aggcagaaac
      721 attccagtag ctcaccagta cgagaaccga attaatgaca gggatgatca gtaccaaggg
      781 ggagatggtc agaaccaagg acttccacct ggaattacaa tggcgcaatt taatgatgtc
      841 tttggtggtg gtcaacctcg aatggttgtt aaagaacggg tcgaccaatg cgttagtgac
      901 ggaaatccaa tgaatgagtg tctgcgtgat gtgaagaatg atatgaggga agagtttaag
      961 agtcacgaaa acttcgacca caataagtat tcggacagaa aggaattgga taatctaaat
     1021 acgaatgctg tacgccaaaa tacgggaaac ccacagggtg gtgatggcca cactatgcat
     1081 acgaaccaat tcggaggtcg tggtgacgga gtcggtaaga ggggagccca agatttcaac
     1141 cagtgcatga ctgacaatac tagtacgggg cctcaatata actcggacgg aaccttcgcc
     1201 agttcaccat cacacggtga cgctgccgat aactgtatgg gccaagcctc aaacacaatg
     1261 cgagattcat atggaaacaa agacatgggt caccatgccg gttttcccca aaatcctcag
     1321 agcggcatga caactcaaca gtagaacaaa aatcgttttc taaactagtg taaaaaaccg
     1381 gttcatggga aatcatgaac cggttttttt attgcgaaaa attcacgtat taatacccaa
     1441 gtggaattgc cgttgttaac ctccggaaaa gcatttaata tcgtagggag atatttagtt
     1501 ccaccagaca cgagaggtca aaatatatga agcagttgat tatgcttttt tccatttcaa
     1561 tcttatccg
//
LOCUS       DASCNS010000196         1029 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001536328, whole genome shotgun sequence.
ACCESSION   DASCNS010000196 DASCNS010000000
VERSION     DASCNS010000196.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001536328"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1029
                     /locus_tag="QF838_07035"
     CDS             <1..>1029
                     /locus_tag="QF838_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HJO60471.1"
                     /translation="NNYMFVDVEGGIQYLNRGQVPIRSFDNAWLPVPGWTGEHEWKGN
                     IPFEEMPRSINPKNGFLVTANNKIIGDQFPYYMALDYAPEFRAKRIVERIEGLEKATV
                     EDMVSIHSERTSIPASVYVPHLLNLNNLNSLQVQAQARLKNWTYEMDPDSISATIYSA
                     FRWKLHLKVIGSLLGSLADQALVSGGRGAPTHINHLAIRLIEGLKNEDSSLLPDGETW
                     DTTISRAFIEGVEYLRQTIGPDLEDWVWGKLHYTKPTHTLSEIFSNHKDLLNPPSVPM
                     GGDGDTPQAGSFALSNPFVMTGMSVARYVFDPSDWDNSRWIVPLGSSGHPGSSHYSDQ
                     TDLWSKVQ"
BASE COUNT          292 a          224 c          232 g          281 t
ORIGIN      
        1 gtaataacta catgtttgtc gatgtggaag gtggtatcca atacctcaac agaggtcagg
       61 ttcctataag aagctttgac aatgcatggc ttccagtccc tggatggaca ggcgagcacg
      121 aatggaaagg taacattcct ttcgaagaaa tgccaaggtc aattaatcct aaaaatggat
      181 ttttagtcac cgctaacaac aaaataattg gagatcaatt tccctactac atggccttgg
      241 attacgcccc tgaatttagg gcaaaacgta tagttgagag aatagagggc cttgagaagg
      301 ccaccgttga agacatggtt tctatccatt cggaacgaac ctcgatacca gcctcagtct
      361 atgtgcctca cctcctcaac ctcaataacc tgaattcctt gcaagtacag gcacaggcaa
      421 ggttaaaaaa ttggacctat gagatggacc ctgattccat ttcagctacg atctattctg
      481 catttagatg gaaattacac ttaaaagtca tcggatcttt gttaggatca ttggcagatc
      541 aggctttggt atccggaggc cgcggagcac caacacatat taatcatctc gctattcggt
      601 taatagaagg gctcaaaaac gaagattctt ccttgctgcc tgatggagaa acatgggaca
      661 cgactatatc ccgagctttt atagagggtg tggaatattt aaggcagaca attgggccag
      721 atcttgaaga ctgggtttgg ggtaaacttc attacacaaa accaacacac acactttctg
      781 agatattcag taatcacaag gatttattga atccaccgtc ggttcccatg ggcggagatg
      841 gtgatactcc tcaggcgggt agttttgctt tgtccaatcc atttgtcatg acaggaatgt
      901 cggttgcccg ttacgtgttt gatccatctg attgggataa ttctcggtgg atagttcctc
      961 tcggttcctc tggccaccca ggtagttcac attactctga ccagactgac ttatggtcta
     1021 aagtacagg
//
LOCUS       DASCNS010000197         7040 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001537847, whole genome shotgun sequence.
ACCESSION   DASCNS010000197 DASCNS010000000
VERSION     DASCNS010000197.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001537847"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /gene="gyrA"
                     /locus_tag="QF838_07040"
     CDS             complement(<1..1981)
                     /gene="gyrA"
                     /locus_tag="QF838_07040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HJO60472.1"
                     /translation="MTSSKSEFGFIKTTSIVDEMRTSYLDYAMSVIVSRALPDARDGL
                     KPVQRRILYAMQDLGMRPGTGYKKSARLVGEVLGKWHPHGDLAVYEAMVRLAQDFTVR
                     MPLVDGQGNFGSIDNDPPAAMRYTEARLSPVSEVMLANLDQETVDWSLNFDDTLREPV
                     VLPARLPNLLVNGASGIAVGMATNIPPHNLREVCNAVNALIDNPEATSEDLMKYVRAP
                     DFPTGGTIMGTSGAREAYTTGKGQIVVRAVAEVEEMPRNANRMQIVVTELPYQVNKAG
                     LVEKIATLAKNRRIEGVSEVRDESDRDGMRVVIELRGGVQPQVVLNNLYKQTPLQSSF
                     SANMLALIDDIPRVITLKIALQQYIKFRQQVVRRRSEFELRKAEERAHILAGLRIAIS
                     NLDEVIKLIRNSQDVENARTGLMATFDLDQPQAQAILDMQLRRLAALEREKLEQEYQQ
                     LQETIKGLQELLGDESKILGVIKDETEEVKSKHGDKRRTTISHDAYDLSREELEAHEQ
                     IVITLSQGGYLKRIQSNTFRRQHRGGRGVSGMNTRDDDPVKELMVVDTHDKLMFFTNK
                     GRVLSKIGYELRADQSRNTRGVPVANIINVWDTESISALINVGKKQYEEYEYLVLGTR
                     QGRVKRINLDDVEHIRPSGLIIMNLKGDDELVSVKL"
     gene            complement(2133..2840)
                     /locus_tag="QF838_07045"
     CDS             complement(2133..2840)
                     /locus_tag="QF838_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019342.3"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HJO60473.1"
                     /translation="MKGAKGPIIGLTCSDVDRSLPYASMVEKFGGVVKLITPSNFNSV
                     SEAMNDIDGLMLAGGADVHPKHYGENSDPGSGSWYNEDLDDMEIPILESALETDLPIL
                     AICRGMQVLNVSMGGSLIQHIEGHNSEQVEGNDRLSSYHRIFLSPGCKLSATVGSGGF
                     VRVNHRHHQGIAEAQKADDLMASAWSMEDGLVEGLESPNHRWVLGVQFHPERRGEIPP
                     HFDKLFETLVFKASSTI"
     gene            complement(2853..3644)
                     /locus_tag="QF838_07050"
     CDS             complement(2853..3644)
                     /locus_tag="QF838_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO60474.1"
                     /translation="MGFETILVEQIGRVGIITLNRPDFLNAFNDDLNRELRLQIRDFN
                     NDPTVGSVVITGAGRAFSAGADVSNFEATVRGESRKSDNETTHFPKWPIFMKESKPTI
                     CAINGYAIGMGITLPLGCDIRLMSESAKLSFRFAYIGLTPEFGSTNLLTQTVGMSKAM
                     EFMLTGRFIEADEALESGLVNHVYPDDQLLTKALELATAIAFNPEWQLSQIKRMMRDH
                     QYNDDLDEVMEIESRFFSQSQRTAAHKEFLTSFREKRSPDYHNLS"
     gene            3746..4795
                     /locus_tag="QF838_07055"
     CDS             3746..4795
                     /locus_tag="QF838_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO60475.1"
                     /translation="MKITEVKAVYPNYKNVVPSWRTHFWQIVVVIKTDQGIVGVGYGG
                     GGVAGVEVVNKHFRELLLGKEIGNTQDIKEVWDQLYSASLPYGRKGIAIMALSGVDLA
                     LWDLLGKAENCPVYELIGDRKKDSVRSYATGTDVEWYAELGFTAHKFPHRWVSDEGIA
                     TAVFAADKARKLLGSKALIMIDTYMSWSFDVTLEMSKSLKEFDIYWFEDVTNPDDLES
                     QAELRNLVKPTLIAGGEHEFTEYGYQEIARTEALDLWQPDITWCGGLTSGIRITEMGN
                     DLGIPVVPHRGGEVWGLHLIVSSTCDDLAEVLPGTKDGNKDDLWLGEPHYLNGYISPP
                     DGPGFGVTVNEALLP"
     gene            complement(4808..5797)
                     /locus_tag="QF838_07060"
     CDS             complement(4808..5797)
                     /locus_tag="QF838_07060"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HJO60476.1"
                     /translation="MNVGTQLKARNDDDYKVFAQLGVNNICGYPPGDPREWTVQVLSD
                     YREHVESFGLKLDFIPLPLNSHEISLAENPNIMLGKSPDRDREIDLIQNIIRNCSLAG
                     IPAVKYNLTLLGVVSTDDEDWRGGSTTRAFRYENALQEPSLTIAGKATEDMMWERINY
                     FVQSVVPIADEYKIRMALHPHDPAMPRPEGFRGVDRVLGSVDGLKKFIDLHSSDFHGL
                     NFCQGTVTEMLENPSEEIFDVIRYFGEREKIFNVHFRNIKGGFLDFVETYIDDGDVDM
                     KKCVELYGELGYPYMLMPDHVPNMSGENSKMVGFAYTYGYIKGLIDSAGFGTG"
     gene            complement(5815..6930)
                     /locus_tag="QF838_07065"
     CDS             complement(5815..6930)
                     /locus_tag="QF838_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO60477.1"
                     /translation="MKISDVSIEVITREVPDTGLDSDLGRFSGPVEQGVLRIFTDEGI
                     EGNCFLGEFRKGSTALYNPILAVLKPELLGMDVAKREWLWSRLGILSARKGLSMSAWA
                     PVDIALWDAAGKQAGVPIHELLGNQRGEIDAYATYPPRHIDSAGYVKEAEEIVGRGFR
                     AYKIHPGVMGTDEVVKMVSMVRERVGPNVKLMFDPNCGYDYRKALDVGQALDGNGFYW
                     YEDPVSHFDLDAIAELTRRLKVPICMSDQPDNQFYWGANALRTRSNRLLRGSAAKLGI
                     TGLKKLCSMAEGFGYNCEIGTAGNSLMNIANLHVIYSVPNCAFYEYWMPAEAHQFGLQ
                     EDVIPNSEGVITATQLPGLGYQIDWDYIEKHKTGTLT"
BASE COUNT         1967 a         1681 c         1329 g         2063 t
ORIGIN      
        1 ccaatttgac agatactaac tcatcatcac ccttaaggtt cataataatc aatccagatg
       61 ggcggatgtg ttcaacatca tcaagattaa ttcttttaac tcgaccttgt cgtgttccta
      121 acacaagata ttcatattct tcatattgtt ttttcccaac atttatcagc gcacttatac
      181 tttcggtgtc ccatacattt attatgttgg ccactggcac gcctcgagta tttctacttt
      241 gatcggccct taattcatag cctatctttg atagaacacg ccctttattt gtgaaaaaca
      301 ttagtttgtc gtgagtatcc accaccatca attccttcac gggatcatca tctctggtgt
      361 tcataccaga aacacctctt ccacctctgt gctggcgcct gaaggtattc gattgaattc
      421 tttttaaata tccaccctgg ctgagagtaa tgacaatttg ctcgtgggct tccagctctt
      481 cacggctgag atcataagca tcatgactta ttgtggttct cctcttatcc ccatgcttgc
      541 tctttacttc ctctgtttca tctttaatga cacccaagat tttactttca tctccaagaa
      601 gctcttgaag tcccttgatc gtctcttgta gctgctggta ttcctgttct aatttttccc
      661 tttcaagcgc tgccaagcgt cttaattgca tatccaaaat agcctgtgcc tgcggctgat
      721 ccagatcgaa agtagccatc agtccggttc tggcattttc gacatcctgt gaattacgaa
      781 tcagcttgat aacctcatca agattgctaa tggcaatcct aaggcctgcc aatatatgcg
      841 ctctttcttc ggcctttctt aattcgaatt cagatctacg tctgacgact tgctgcctaa
      901 attttatgta ttgctgtaag gctattttga gtgttattac tctcggtata tcatcgatga
      961 gagctaacat attggccgag aatgaagatt gaagtggggt ttgtttgtac aagttattca
     1021 acacaacctg aggttgaaca cctcctctca gttcaataac tacgcgcatt ccatccctgt
     1081 cggattcgtc acgaacctcc gacaccccct caattctgcg attttttgct aaggtagcta
     1141 ttttttccac taacccagct ttgttgacct ggtaaggcag ttcagttact actatctgca
     1201 tcctgttggc atttcgaggc atctcttcca cctctgcgac agctcgtaca acaatctgcc
     1261 ctttccctgt cgtgtaggcc tctctcgcac cactagtgcc cattatggta cccccagtgg
     1321 gaaagtccgg ggctctaacg tatttcatta aatcttccga agtggcttcg ggattatcta
     1381 taagagcatt cacagcatta caaacctccc tcaaattatg agggggtata tttgttgcca
     1441 tacccaccgc aattcccgat gcaccattca ctaaaagatt gggaagcctt gctggtaaaa
     1501 caacaggctc tctaagagtg tcgtcgaaat ttagtgacca atcaacggtt tcctgatcca
     1561 agttggctaa cataacttcc gacacagggc tcaagcgagc ctcagtgtat ctcattgcag
     1621 caggtggatc gttatcaata cttccaaagt taccctgtcc atcaaccaac ggcatcctaa
     1681 ctgtaaaatc ttgagccagt ctaaccatcg cttcatagac agccagatct ccatgagggt
     1741 gccattttcc taaaacttcc ccaactagcc tcgcgctttt tttatacccg gtgcctggtc
     1801 tcatgccaag atcctgcatc gcatacaaaa tacgtctttg aactggtttc aacccatctc
     1861 tcgcatcagg tagtgctctt gagacaatta cactcatagc atagtccaga taagaggttc
     1921 tcatctcatc tactatggag gtggttttta taaatccaaa ttctgacttg ctagatgtca
     1981 taaattaata ctccgacaga aacccaaatt ttcgactatg ccaatcatca atgaaatagg
     2041 ttctggttaa taatatgaag gcaacaattt cactattttg ctgaccttga ttatagcaca
     2101 aaaagtagtg gttttatgac tataaaaccc ctctatatag tggatgaagc cttaaaaact
     2161 agagtttcaa acaatttgtc gaaatgtggg gggatttccc ctcttctttc agggtggaat
     2221 tgaacaccta aaacccagcg atgattcggg ctttcaaggc cttctaccaa gccatcttcc
     2281 atcgaccagg cagaggccat aagatcgtcg gctttttgcg cctctgcaat cccctgatgg
     2341 tgtcgatgat tgaccctaac aaacccacca gaaccaaccg tagcggataa tttacagcca
     2401 ggagacagaa aaattcgatg gtagctagaa aggcggtcat ttccttctac ctgttcgcta
     2461 ttatgacctt cgatgtgttg aatcaaggac ccgcccatcg aaacgttcaa aacctgcata
     2521 cctctacata tggctaaaat cggcaaatct gtttcaagtg cggattccaa tataggtatt
     2581 tccatatcat ccagatcttc gttataccag gaaccggagc cgggatcaga attttcaccg
     2641 tagtgtttag ggtgtacatc tgccccacca gctaacatta gtccatctat atcgttcata
     2701 gcttcagaga cactattgaa atttgatggg gtaataagtt tcaccacacc cccaaatttt
     2761 tctaccatgg atgcgtaagg gagtgatcta tcaacatctg aacatgtcag acctatgatt
     2821 ggccctttag ctcctttcat atagacaccc cttcaggata aattatgata atcaggagat
     2881 ctcttctccc gaaaagaggt aagaaattct ttgtgggctg ctgttcgttg tgactgacta
     2941 aagaatctac tttctatctc cattacctca tcaagatcat cgttatattg atgatctctc
     3001 atcattcttt taatttgact gagctgccac tcgggattga aagctatagc agtagccaac
     3061 tccagagctt tagtaagtaa ttggtcatcc gggtagacgt gattaaccaa tccagattct
     3121 aaagcctcat cggcctcaat aaaacgacct gtaagcataa attccatagc tttggacatg
     3181 ccaactgtct gagttaaaag attggtgctg ccgaattctg gggtcaatcc aatgtatgcg
     3241 aatcgaaacg agagttttgc actttctgac ataaggcgaa tatcgcaacc caatggcagt
     3301 gttataccca taccgatagc atacccgtta atggcgcaaa tagtaggttt ggattctttc
     3361 ataaaaatgg gccatttggg aaagtgagtt gtctcgttgt cagattttct tgattccccc
     3421 ctgacagttg cttcaaaatt gcttacatct gctcctgcag aaaaggctct ccctgctcct
     3481 gtaatgacta cagagccgac tgtaggatca ttattaaaat ctctgatttg tagccgcaat
     3541 tctctgttga gatcatcatt aaaagcgttt aaaaaatcag gtctgttcaa agttatgata
     3601 cctacccttc cgatttgttc tactaagata gtttcaaatc ccattacaac tcccctctta
     3661 atgtttacat agaatcaagt taccatagcc gtaactacct aacaacataa aacctatact
     3721 aattcagaat ggtaggatga agttaatgaa aattactgaa gtgaaagcag tgtatcccaa
     3781 ctataaaaac gtagtcccct cttggcggac acacttttgg caaatagttg tggtgattaa
     3841 aacagatcaa gggattgtag gagtaggata tggaggggga ggtgtagccg gtgtggaagt
     3901 tgtgaataag catttcaggg aacttctttt aggcaaggaa ataggcaaca cccaagatat
     3961 aaaagaggtc tgggaccagc tttattcagc ttctctaccc tatggcagaa agggtattgc
     4021 aataatggcc ttaagcggag tggacttagc attgtgggat ctgctcggaa aggcggaaaa
     4081 ttgtccggta tatgaattga taggagaccg aaaaaaggat tctgttcgat cttatgctac
     4141 tggaaccgac gttgaatggt atgcagaatt aggtttcact gcccataaat ttccgcatag
     4201 gtgggtctct gacgaaggta ttgcaaccgc agtattcgct gcagataaag caagaaaatt
     4261 attaggctca aaagcattaa ttatgatcga tacatacatg tcatggagct ttgacgttac
     4321 cttagagatg tcaaaatctt tgaaagaatt cgatatttat tggtttgaag atgtaactaa
     4381 ccctgatgat ctagagtctc aagctgaatt aagaaactta gttaagccta ctttgattgc
     4441 aggtggagag catgaattca ccgaatatgg ctaccaagaa atagccagaa cagaggcctt
     4501 agacctttgg caacctgata taacatggtg cggaggcctg acatctggca ttcggatcac
     4561 tgagatggga aatgacctcg ggataccggt cgtgccccat agaggggggg aagtgtgggg
     4621 acttcactta attgtctctt caacctgcga cgacttagcc gaggtacttc caggtaccaa
     4681 ggatggtaac aaagacgacc tgtggctagg ggagccacat tacctgaatg gatatatctc
     4741 accaccagat ggcccaggat ttggagttac cgttaacgaa gcattacttc cttaagtaat
     4801 aaaaagttta ccctgtccca aacccagcag aatcgattaa tcccttgatg tatccgtagg
     4861 tatatgcaaa ccccaccatt ttagaatttt ccccagacat gttcggtaca tgatcgggca
     4921 tgagcatata agggtaacct agctccccgt acagttctac acactttttc atatcgacat
     4981 ctccatcatc aatataagtt tctacaaaat caaggaaccc acctttaata tttcggaaat
     5041 ggacattgaa tatcttttcc ctttcgccaa aatatctaat aacgtcaaaa atttcctctg
     5101 atgggttttc taacatttct gtgacagtac cttggcaaaa attcaatccg tgaaaatcgc
     5161 ttgagtgcaa atcgatgaat ttcttcaacc cgtcaacact tcctaaaact cggtcaacac
     5221 ctctgaaacc ttcaggtctg ggcattgctg gatcgtgagg atggagggcc atccgaatct
     5281 tatactcatc cgcaattggt actactgatt gaacaaagta atttattctt tcccacatca
     5341 tatcctctgt ggccttgccc gcgatcgtta gactaggttc ttgtagagca ttttcgtatc
     5401 taaatgctct ggtggtagac cctccccgcc aatcttcgtc atcagtactc actacaccta
     5461 aaagggtcaa attgtactta acagcaggta taccggctaa tgaacaattc ctaatgatgt
     5521 tttgtatcag gtcaatttct ctatctctat caggagattt tccaagcata atattaggat
     5581 tttccgctaa ggatatctca tgagaattga gtggcaatgg gataaaatcc aacttcaacc
     5641 caaaagattc gacatgttct ctatagtcac tcaagacctg tacagtccat tctcgaggat
     5701 cacctggggg gtatccacaa atattattta ccccaagttg ggcaaatact ttgtaatcat
     5761 catcatttct ggccttgagc tgtgttccaa cattcattta attcaccaat cgcactaagt
     5821 taatgtacct gttttatgtt tttctatgta atcccaatcg atttggtatc ccaaaccagg
     5881 caattgggtt gctgtaatga ccccttcgct attcggtata acatcctctt gaagcccaaa
     5941 ttgatgggct tccgcaggca tccaatattc atagaaagca caatttggta ctgagtaaat
     6001 cacatgcaaa ttagctatgt tcataaggga gttcccagct gtgcctattt cgcagttata
     6061 accaaatcct tccgccatgg aacataattt cttaagtcct gttattccca acttggcagc
     6121 actacctctg agcaacctat tggacctcgt cctcagggca ttagctcccc agtagaattg
     6181 attatctggc tggtcgctca tgcaaatagg aactttcaat ctccttgtca attcagcaat
     6241 cgcgtctaaa tcaaaatgag aaacaggatc ttcgtaccaa tagaacccat ttccatctaa
     6301 agcctggcct acatctaatg ccttccggta gtcatatcca cagtttggat caaacataag
     6361 cttcacatta ggacctactc gctctctgac catactcacc attttcacaa cttcatcagt
     6421 ccccattacg cctggatgta tcttataagc cctgaaacct ctaccgacga tctcctccgc
     6481 ttcttttaca tacccagcac tatcaatatg gcggggcggg taagtagcgt aagcatcaat
     6541 ctcacctctt tgattcccaa gcagctcgtg aatgggaaca ccggcttgtt ttccagcagc
     6601 gtcccacagt gcgatatcaa cgggagccca agctgacata cttaggcctt ttcgggccga
     6661 taatattccc aacctactcc acaaccattc ccgcttcgca acatccattc ccaacaattc
     6721 aggtttaaga accgccaaaa tagggttata gagagcagta gaaccctttc ggaattctcc
     6781 caaaaaacaa ttcccttcaa ttccttcatc agtaaaaatg cggagaaccc cctgttcgac
     6841 agggccagag aacctcccaa gatcagaatc cagtcccgtg tcaggtactt ctcttgttat
     6901 aacttcaata ctgacatccg aaattttcat ctaactatcc tccaaagggc tttctagccc
     6961 gattatataa gaattcacag tgttaaaatt ccctgatgga attgttgaaa cgcgctgggt
     7021 cgtggacacc taaaaccact
//
LOCUS       DASCNS010000198         9161 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001549991, whole genome shotgun sequence.
ACCESSION   DASCNS010000198 DASCNS010000000
VERSION     DASCNS010000198.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001549991"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="QF838_07070"
     CDS             <1..315
                     /locus_tag="QF838_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012787.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HJO60478.1"
                     /translation="PPCVAEFPQIDQFYLKNRNSVKVIGIQLLNLDSAQDGQDFVKEK
                     NIHFAVGADPTGKVSVDYQIKVYPTTVFVNSQGLITGHWQGEITMEELDQQLLLLQNK
                     AN"
     gene            849..1031
                     /locus_tag="QF838_07075"
     CDS             849..1031
                     /locus_tag="QF838_07075"
                     /inference="COORDINATES: protein motif:HMM:NF038101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HJO60479.1"
                     /translation="MKKTFLETIRCPLCKSNFALHSDSEKDNEILEGKLICSQCKEEF
                     PIVNSVPNLLPPDLRL"
     gene            complement(1042..1524)
                     /gene="folK"
                     /locus_tag="QF838_07080"
     CDS             complement(1042..1524)
                     /gene="folK"
                     /locus_tag="QF838_07080"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HJO60480.1"
                     /translation="MGLGSNIGDRLGNITNATLMLQKLAHTFTSSRLYETTPYGFSKQ
                     PAFLNSVCKLSTELTPWIFLHHVKEIEKNLQRHRVFTNSPRTIDVDILMWGDIVLESQ
                     LLTIPHPRFHERMFVLDPLFEISPTLVHPLLNLTVGEMRQVVQKKEPLEIQASSLRLF
                     "
     gene            complement(1548..2327)
                     /gene="folP"
                     /locus_tag="QF838_07085"
     CDS             complement(1548..2327)
                     /gene="folP"
                     /locus_tag="QF838_07085"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HJO60481.1"
                     /translation="MGILNVTPDSFSGDGIHQNVNLSISKGIDMLSNGADVLDIGGES
                     SKPPSIYGHVDKVAADEEIQRIVPVIRALSLESETIISVDTYKAKVAKAAISAGATMV
                     NDIWGLRRDPEMVQLIAATGVYVVIMHNAESAQYKDIIKDNVEFLKKRVGFAISSGIE
                     THKIIVDPGIGFGKGSEQNLQILNRLDEYKTIGVPIMVGTSRKSTIGEILELPVDDRI
                     EGTAATVSVAITRGADMVRVHDVPEMHRIAKMTDAIVRGGA"
     gene            2517..4079
                     /locus_tag="QF838_07090"
     CDS             2517..4079
                     /locus_tag="QF838_07090"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018258608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HJO60482.1"
                     /translation="MSQENSTSKKHEELLDKKALSLKGGGDKRVEAQHKRGKLTARER
                     ISLLLDDGSFEELDPLVLHRSTNFGLDNQHFLGDAVVTGYGEVNGRLTFVYAQDFTVL
                     GGSLSEAVANKICKVMDKAIESGAPIVGLIDSGGARIQEGVDSLSGYGSIFHRNVMSS
                     GVIPQISVMMGPAAGGATYSPALTDFIFMVEGLGQMYITGPDVIRAVTSEDISHEDLG
                     GTEAHTVKSGVAHFAEETEESCIARVQELLSYLPQNNMEDSPLTSTGDSPDRYSDNLN
                     SLIPDNPNQPYNMLDVIHTVVDESNFLQVHKNFAPNILVGFARFDGKAVGIVAQQPDH
                     FAGVLDIDASVKAARFVRFCDAFNIPLVTFIDVPGFMPGSSQEYGGIIRHGAKLIFAY
                     AEATVPKISVLTRKAYGGAYIVMSSKNLKGDVNYSWPTGQVAVMGAEGAVNIIHRSQI
                     RESDDPKELTDQLVSEYEENLMNPYIAASRGMIDDVIEPGQTRLKIIRALKMLANKRE
                     NIPPKKHGSIPL"
     gene            4298..6148
                     /locus_tag="QF838_07095"
     CDS             4298..6148
                     /locus_tag="QF838_07095"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HJO60483.1"
                     /translation="MNTFNSFGITDTTFRDAHQSLLATRLEMKDMEPIAAQMDLVGFA
                     SMEVWGGATFDATTRYLYEDPWERLRQFKKLITNTPLSMLLRGQSLVGYKAYADDVVD
                     AFVERSAINGIDIFRVFDALNDEWNLSRAAEAVKNNDKHLQMTICYSVTENGKMGGPI
                     YNLDYYLDKAKVFQDMGADSLCIKDMAGLLSPYDAFDLISALKKVVTVPLQLHTHYTS
                     GMASMTVLKAIEAGVDVVDACLAPLALRTSQPAVEPLVVALRGTENDPKLDLETLINL
                     GDYLESVLPKYSEYLETPKASVIDVKVLSHQIPGGMASNLISQLREAGAIDRLDEVLE
                     DIPLTRKELGYPPLVTPMSQMVGSQAVSNVLFGRYKMVSSQMKEYLTGMYGRPPSKVD
                     NQIKDQIFESNDTAKIKTINERPGNLIEPELKQAEKEVENITTDTDDILIYALYPTTG
                     MKFLRVKHGVDVQKNDIGLNADGDTSGTHEIAATGSQEVPVKSLKARSFNVFIDDTFY
                     KVEIDPVDQNVVSISDGIPNPTLARDPKPTLNGSSANSDGELKSPMPGVILKYLVEVG
                     QQVNQGDPIAVLEAMKMENTLPSPKDGRIQSLTVEPGQTVVKGDLLAIIT"
     gene            6212..7144
                     /gene="mdh"
                     /locus_tag="QF838_07100"
     CDS             6212..7144
                     /gene="mdh"
                     /locus_tag="QF838_07100"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HJO60484.1"
                     /translation="MRNKVTVVGAGNVGATTAQRIFDGGYADVVLVDIVEGLPQGKAL
                     DMLESGPILGTDSSIVGTNGYEETEGSDVVVVTSGIARKPGMSRDDLLLTNMKIVTSV
                     VEQTSKFSPNSVHLIVTNPLDAMAQQALKVTGFPSNQVVGMAGILDTARYRTFLAEEL
                     GVSVNSVSAYVLGGHGDTMVPVVESTNVAGTPVSKLIAPERLEEIVQRARDGGAEIVN
                     YLKTGSAFYAPSASIVQMVEAIIFDKKEILPCTAYLQGQYGIDDLYVGVPVKLGSKGV
                     EEIIELNLNGDELKALRKSAEAVQELVEVMAGAQ"
     gene            7178..7648
                     /locus_tag="QF838_07105"
     CDS             7178..7648
                     /locus_tag="QF838_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HJO60485.1"
                     /translation="MIIAVIGESSASPENTKLAESVGSLLAKANITVVCGGQGGVMRA
                     VCKGAKKHGGTTIGILPGMDSLDSNEYVDIPISTGLGNGRNLLVTRAGKSVIAIGGSY
                     GTLSEIGLALSDSKPVIGLNTWSLLRDEHLDCGIIVADSPEEAVELAICQANKN"
     gene            7662..8954
                     /gene="eno"
                     /locus_tag="QF838_07110"
     CDS             7662..8954
                     /gene="eno"
                     /locus_tag="QF838_07110"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HJO60486.1"
                     /translation="MTSIYSIRAREILDSRGNPTVEVDVVLEDGSVGTSAVPSGASTG
                     QYEAIELRDGDKNRYHGKGVLKALDNVQNVIRPNVIGFDAYDQENLDSKLIELDGTEN
                     KSNLGANSILAVSLATAKAAAISSKKELFAHISKSDEYLIPVPMLNVINGGKHAENST
                     DIQEFMIVPVGFRTFSDAIKAGSEVYQSLQKNLSESNLSTTVGDEGGFAPQLPSNESA
                     LEILVDAITSTGYECGRHFLIALDVAAAELFDSESKLYNLVREKKIVSGEQLSEMYST
                     WVEKYPIISIEDGLSDDEWNDWEKMTRRVGSKVQLVADDLLTTNPIRISKGIESKAAN
                     AVLIKPNQIGTLSETLEAISLVQSVGWGTVISHRSGETEDTFISDLAVATNSGQIKSG
                     APARGERTAKYNRLIRIEESLGNRCRFAGGSVYSRFIS"
     gene            9002..>9161
                     /locus_tag="QF838_07115"
     CDS             9002..>9161
                     /locus_tag="QF838_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012274.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HJO60487.1"
                     /translation="MKARIGINGFGRIGRLVLRAVIQEGYKDIEVGAINSFADPESNA
                     HMFKYDTTY"
BASE COUNT         2869 a         1857 c         1993 g         2442 t
ORIGIN      
        1 ccaccttgtg tagctgagtt cccccaaata gatcaatttt atttgaagaa ccggaattcc
       61 gtaaaagtta taggaattca attacttaat ttagactcgg ctcaagatgg gcaagatttt
      121 gtgaaggaaa aaaatataca ttttgcggta ggagcggacc ccacagggaa agtgtctgtt
      181 gattatcaaa tcaaggtgta cccgactaca gtgtttgtaa actcccaagg cctgataaca
      241 ggtcattggc aaggtgaaat cactatggaa gaattagatc aacaattgtt gcttctacaa
      301 aataaagcga actgaaaatt gaatatatcg gacctattta atatttttat acggtgggca
      361 catctcattt ccgcaactgc ctgggtaggt ggaagtatat tttgggtttt agttctaaat
      421 ccttccatga agcattctga aatttcgtta attaaaaaat tccactctca attatctacc
      481 gaatttagag ggttggtcga cacttgtata tttgtacttt tagccactgg agcaataatg
      541 acatttaaca gattaactcc tggcaatatt ggttacccat acgcattaat attagggcta
      601 aaaatcgttt tcgtatttgg aatgttttac tttataaggg ccaaacgcca cttgaatttc
      661 atttcaaaat tcagtctaac tgagcatcat gaaaagccca aatcaaaagg actcacacgg
      721 ttcctaaacg gatacaatat tgcgctgatt ctagggcttt ctgtttatct actttcgaat
      781 ttgttaaacc tgatatacat atcaacaatt tcttgacaaa tgtaatcttc ataacagagg
      841 ttcataaagt gaaaaaaacg tttttagaaa ccatcagatg cccattatgt aaatcaaatt
      901 tcgcattaca ttcagattca gaaaaagata acgaaatctt ggaagggaaa ttaatatgtt
      961 cccaatgcaa agaggaattc ccgattgtta attcagtccc aaatcttcta cctccagact
     1021 tacgtttata aattgagttc attaaaataa acgtagagaa gaggcctgta tctctaatgg
     1081 ttcctttttt tgaactacct gccgcatttc accgactgtt aaatttagaa gcggatgcac
     1141 caaggtaggc gagatttcaa acaagggatc tagcacaaac atcctttcgt ggaacctggg
     1201 atgtggaata gtcaaaagtt ggctttcaag tacgatatca ccccacatca aaatatctac
     1261 atcaatcgtc ctaggtgagt tggtaaaaac cctatgtctt tgaaggtttt tctctatttc
     1321 cttcacgtgg tgtagaaaaa tccacggagt taactcggta gataatttac acacagaatt
     1381 caagaacgct ggttgtttgg aaaacccata aggggtggtt tcgtacaatc tagaagaagt
     1441 gaaagtatga gccaatttct gcagcatgag agtagcattg gtaatattgc ccaaccggtc
     1501 acctatatta cttcccaaac ccacatatac atttgttgaa aaagtaatta tgctccccct
     1561 cttactatgg catcggtcat cttggctatt ctgtgcattt ccggaacatc atgtactctg
     1621 accatatcag ctcccctagt tatagctacc gatacggtcg cagcagttcc ttcgatacgg
     1681 tcatcaactg gcaattccaa aatttcgcca atcgttgatt tacgagaggt tcctaccatg
     1741 ataggcactc ctattgtttt atattcatcc agcctattca aaatttgaag gttttgctct
     1801 gatccttttc cgaaacctat acctggatcc actattattt tgtgtgtttc aatgccagat
     1861 gaaatggcaa aaccaactct ctttttcaaa aactctacat tatctttgat aatatcttta
     1921 tattgagctg attctgcgtt gtgcatgatc accacataga cccctgtagc cgcaataagt
     1981 tgaaccatct caggatctct tctcagcccc caaatatcat ttaccattgt ggctcctgca
     2041 gaaatagctg ctttagctac tttagctttg taggtgtcta cactgattat cgtctccgat
     2101 tcaagactca aagcacgtat gacaggaaca atcctttgga tttcctcatc cgccgcaact
     2161 ttatccacat gcccataaat agacggaggt ttagaagatt caccacctat atccaacacg
     2221 tctgctccgt ttgacaacat gtcaatcccc ttcgatatac tcaggttcac attctgatga
     2281 ataccatcgc cagaaaatga atcgggagtg acgttcaata tccccatcag gtatgttttg
     2341 gatccccagt caaacaaggt caattcagtt taatccttcg ttacaatgtt attcggataa
     2401 accacactat ttcggtagac ttagatactt atttacagtg agctataata aattctacat
     2461 atatatattt gcatcaaatt aatcttataa agtagttcac attttaatcc agccatatgt
     2521 cacaagaaaa ctccacatca aaaaaacacg aagaattatt ggacaaaaaa gccttaagct
     2581 tgaaaggcgg tggcgataaa cgtgttgagg cccaacacaa gaggggtaaa ttaacagctc
     2641 gagaacgtat ctctttacta ctagatgatg gttcctttga agaattggac ccgctggtac
     2701 tacatagatc cacgaatttc ggcttagaca accagcattt tctaggtgat gctgtagtca
     2761 ctggttacgg agaagtcaat ggcagactta cttttgtcta cgcacaggat tttactgttc
     2821 tgggtggttc attatctgaa gctgtagcca acaagatctg taaggtaatg gacaaagcca
     2881 ttgaaagtgg ggccccaata gtcgggctaa tagattcagg aggtgcaaga atccaggaag
     2941 gcgttgacag tctttcaggc tatggctcca tttttcatag aaacgtgatg tcttcaggtg
     3001 taataccgca gatatcagtc atgatggggc ctgctgctgg tggtgctact tattctccag
     3061 ccttgaccga tttcattttc atggtggaag gattaggcca gatgtacatt accggacctg
     3121 acgttatacg ggcagtaact tctgaagata tatcgcatga agatttagga ggaactgaag
     3181 ctcacacagt taaaagcggc gttgcccatt tcgcagaaga aacggaagaa tcatgcattg
     3241 cgagggtcca agaattatta agttacctac cacaaaacaa tatggaagat tcccccttaa
     3301 ccagtaccgg tgattctccc gatagatact cggacaattt aaacagcctg ataccagata
     3361 acccgaacca accttataac atgcttgacg tcattcacac tgtggtggat gaaagcaatt
     3421 ttttgcaagt ccacaagaat tttgcaccta atatattggt tggattcgca aggtttgatg
     3481 gaaaagcagt aggaattgtc gcacaacagc ctgaccattt cgcaggcgta cttgatatag
     3541 acgcatctgt gaaagctgca aggttcgtaa gattctgcga tgccttcaat atacctttag
     3601 tgacttttat agatgttccc gggttcatgc ccgggagttc tcaggaatat ggaggaatta
     3661 tcagacatgg agcaaagtta atattcgcct acgcggaagc cactgttcca aaaataagcg
     3721 ttttaaccag aaaggcttat ggcggcgctt atatcgtgat gagtagtaaa aacctgaaag
     3781 gggacgtgaa ttactcatgg ccaacaggcc aagtcgcagt aatgggtgcg gaaggtgccg
     3841 ttaatataat acatagatcc caaataagag agtcagatga ccctaaagaa ttaactgatc
     3901 agttggtctc agaatacgaa gaaaacctca tgaatcccta catcgctgca agcagaggaa
     3961 tgatagacga cgtgatagaa cctggtcaga cacggttaaa aatcatcaga gccttgaaaa
     4021 tgttggctaa caaaagagaa aatatacctc cgaagaaaca cgggagcatc cctttataaa
     4081 cgatatgaaa ttaactcaga aacatatggc tgccatatca actgccattc aaatgtattt
     4141 agatgaccag aaagctttag actcaaaaag tgataactta atatcttcat ggaaacacgc
     4201 aagtctttat cctcttcctt attcgcataa tcgtgcaatt ggaggatgga gcggaaggcc
     4261 tcctactcat aagtaacgca aggattcacc tgactatatg aacacattta actcttttgg
     4321 catcactgac acaaccttcc gagacgccca ccaaagcctt cttgccacaa gactcgaaat
     4381 gaaagatatg gaacccattg cggctcaaat ggacctggtc ggttttgcgt ccatggaagt
     4441 atggggtgga gctacctttg atgctactac cagatatctt tatgaagacc cctgggaaag
     4501 gttgcgccag ttcaaaaaat taataacaaa cactccctta tcaatgttac taagagggca
     4561 atctttggtt gggtataaag cgtacgctga tgacgtagta gatgcgtttg ttgaacgctc
     4621 tgcgataaat ggaatagaca tttttagggt gtttgacgca ttgaacgacg aatggaacct
     4681 gtctcgagct gctgaagctg tgaaaaataa tgacaagcat ttacaaatga caatttgtta
     4741 ctcggtcact gaaaacggga agatgggcgg accaatatat aatttagact actatctgga
     4801 taaagcaaaa gttttccaag acatgggggc agatagcctt tgcatcaagg atatggcagg
     4861 actcttatca ccttatgatg ccttcgattt aatttcagcc ttgaaaaagg tggtgacagt
     4921 acctctccaa ttgcacactc attacaccag tggcatggcc tccatgaccg ttttaaaagc
     4981 gattgaagca ggggtagacg tagttgatgc ttgcctagct ccattagcgc tgagaacttc
     5041 acagccagct gtagaaccgc ttgttgttgc tctgcgaggc acagaaaatg atcctaaatt
     5101 ggatctagaa acccttatca atttagggga ctatctagaa tcagtcttac cgaagtacag
     5161 cgaatacctg gaaactccca aagcctctgt gatcgacgtt aaagttctga gccatcaaat
     5221 acctggcgga atggcaagta acttgatatc gcaacttaga gaagctggcg caatagatag
     5281 gcttgatgag gtgttggaag atatccccct cactaggaaa gaactagggt atccaccatt
     5341 ggtgacgccg atgagtcaga tggtgggatc tcaagcagtt agcaatgtgc tttttggtag
     5401 atacaaaatg gtatccagtc aaatgaagga gtatctcact gggatgtacg gtaggccccc
     5461 ttccaaggtg gataaccaga ttaaagacca aatcttcgaa tccaacgata ctgcaaaaat
     5521 aaagacgatc aatgaaagac ccggaaacct gatagagcct gaattaaaac aagcagaaaa
     5581 agaagtggag aacatcacga ccgatactga tgacatccta atttacgcct tataccccac
     5641 tacgggaatg aaatttctca gggtaaagca tggggttgat gtgcagaaaa atgacattgg
     5701 tctaaatgct gatggtgaca cttcagggac acatgaaatt gctgcaacag gatcacagga
     5761 agtaccggtt aaaagtttaa aggcacgatc cttcaatgtt tttattgacg acacctttta
     5821 taaggtggaa atcgatcccg ttgaccaaaa tgtggtttca atttctgacg gcattccaaa
     5881 tccaacatta gcgagagacc caaaacccac attaaatgga tcatcggcaa attccgacgg
     5941 agaattaaaa tcccctatgc ccggcgtgat tttgaagtac ttagtagagg taggccagca
     6001 agtaaaccaa ggtgacccta tagcagtttt agaagcgatg aaaatggaaa acaccctacc
     6061 ctcgccaaaa gatgggcgaa tccaatcatt gacggtagaa cctgggcaaa ctgttgtaaa
     6121 gggcgacctg ctagctataa tcacctaatc aaactcgttt gtaattatta tcgcaatcat
     6181 aattgctaaa acacacggaa agaggaatga aatgagaaac aaagtgactg ttgttggggc
     6241 tggaaacgta ggtgccacca ccgctcaacg tatttttgac ggaggctatg ctgacgtcgt
     6301 tttggtggat attgtggaag ggttacctca aggaaaggct ttagacatgc ttgaatctgg
     6361 accgatcttg gggacagatt caagcattgt aggtaccaat ggatatgaag aaactgaagg
     6421 ttctgacgtt gttgttgtaa cctcaggaat agcccgcaaa cctggcatga gtcgagacga
     6481 tttactactc accaatatga aaatagtcac cagcgtagtg gagcaaactt ctaaattctc
     6541 tcccaatagc gtccacttaa tagtcacaaa ccctctggat gcaatggctc aacaagcttt
     6601 gaaagttact gggttcccga gtaatcaagt ggtaggcatg gctggcatcc ttgatacagc
     6661 tagatacaga acttttctcg cggaagaatt gggagtgtcc gtcaattctg tctcagccta
     6721 tgtacttggc ggccatggag acacaatggt acctgtagtg gaaagtacca atgttgcagg
     6781 aacaccagta tccaaactga tagctccgga aaggttggag gaaatagtac aaagggcgag
     6841 ggacggcggt gcagaaatag tgaactattt gaagactgga agcgcttttt acgccccttc
     6901 ggcatcgata gttcagatgg tagaggccat tattttcgat aagaaagaga ttcttccttg
     6961 tacagcatat ttacaaggtc agtacggcat cgatgatctc tatgttgggg tgcctgtcaa
     7021 gctcggttca aagggagtcg aagagataat tgagttgaat ttgaatggag atgagctcaa
     7081 ggccttaagg aaatccgcgg aagcagtaca agagttggtc gaggttatgg caggagctca
     7141 atagagtttc taaaagacat ctcgaataag gtgatatttg ataattgctg taataggtga
     7201 aagttccgct tctccagaaa acaccaagct tgctgagtcg gtaggctctc ttttggcaaa
     7261 agccaacata acggtggttt gtgggggcca aggaggagtt atgagagccg tttgcaaagg
     7321 agccaaaaaa cacggtggca ccaccatagg aattttgcca ggaatggact cgctcgattc
     7381 caacgaatat gttgatatcc ctatcagtac cgggcttgga aatggtcgca acttactagt
     7441 tacaagggct ggaaaatcgg tcatcgcgat cggcggttcc tacgggactt tgtctgaaat
     7501 tggcctggca ctatcagact caaaaccagt aattggccta aacacgtgga gtttactcag
     7561 ggacgaacat ttagattgtg gaataatagt agcggacagc ccagaagagg ccgttgaact
     7621 cgcgatctgc caagctaaca aaaattaagg accattctaa aatgacttca atttatagca
     7681 tcagggccag agaaatcctc gattcaagag gaaaccctac ggttgaggta gatgtagtct
     7741 tagaagacgg ttccgtaggc acatctgccg tgccatcagg tgctagtact ggacaatatg
     7801 aggcaattga actccgagat ggtgataaaa acagatacca cggaaaagga gtgcttaaag
     7861 cactcgataa tgttcagaat gttatacgtc caaacgtcat cggcttcgat gcctatgatc
     7921 aagaaaattt agattctaaa ttaattgaac tagatgggac cgaaaataaa agtaatttag
     7981 gcgcaaattc catattggca gtatcactcg ctaccgccaa agctgccgct atttcatcta
     8041 agaaagagct attcgctcat atttcaaaat cagatgaata cttaattcct gtccctatgc
     8101 tgaatgttat taatggcggc aaacatgctg aaaattccac cgacattcaa gaattcatga
     8161 tagtgcctgt cggatttaga accttctctg acgctatcaa agccggctcc gaagtatatc
     8221 aatcgcttca aaaaaatctg agcgaaagca atctcagtac cactgttgga gatgaaggtg
     8281 gtttcgcccc gcagttacca tccaacgaat ctgcattgga aatattagtt gacgccataa
     8341 cttctactgg atatgaatgc ggtcgtcatt ttcttatcgc attagatgta gcggcggcag
     8401 agctttttga ctcagagagt aaactctaca atttagtgcg tgaaaaaaaa attgtttcag
     8461 gcgaacaact gagtgaaatg tattctacct gggtagaaaa atatccgatc ataagcatag
     8521 aggatggctt atcagatgat gaatggaacg actgggaaaa aatgacacgg cgcgtgggca
     8581 gcaaggtaca gctggtcgca gatgaccttc ttacaacgaa cccaatcaga atatcaaaag
     8641 gcatcgaaag taaggcagcc aatgctgtac ttataaagcc aaaccaaatc ggtactttga
     8701 gtgaaacatt agaggccatc agcttagtcc aatctgtagg atggggtaca gttataagtc
     8761 acaggtcggg tgaaacagaa gacactttta tatctgactt agccgtagct acaaattcgg
     8821 gacagataaa atctggggcc cctgccagag gcgaaagaac tgctaagtac aaccggctca
     8881 tacgcattga ggaaagttta ggcaatcggt gtaggtttgc cggaggatcc gtttactccc
     8941 gtttcataag ttagttaaat atgaaaaaag agactcatta gactaaaagg gtttgaatta
     9001 catgaaggcg cgaattggta tcaacggttt cggaagaata ggtagattgg tattgagggc
     9061 cgtaattcaa gaggggtata aagatatcga ggtaggagct attaatagct ttgcagaccc
     9121 cgaaagtaat gcccatatgt ttaaatatga cacaacctat g
//
LOCUS       DASCNS010000199         8476 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001553118, whole genome shotgun sequence.
ACCESSION   DASCNS010000199 DASCNS010000000
VERSION     DASCNS010000199.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001553118"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="QF838_07120"
     CDS             complement(<1..701)
                     /locus_tag="QF838_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HJO60488.1"
                     /translation="MKINLLVMIVLTILLVVLTPLLVMGIINLGNNIKPDEDIKSEKQ
                     VGLEKNEGIESELPPSTTTLILTYPSPIPTYTPLPTYTPVPTYTPLPTYTPYPTTPPT
                     PTTTPASTPTPTNTPIPTLTPINTETVATTKFANQRYAFTPLIGLCDFSSSLIPKLSS
                     EELAFMNKNKLSEVVRFFPSKIFDDETTKIVTTGNIETFPVKLHDDGTHGDDIANDGL
                     FSRACISKTDLEITLT"
     gene            complement(783..1577)
                     /locus_tag="QF838_07125"
     CDS             complement(783..1577)
                     /locus_tag="QF838_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020022195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO60489.1"
                     /translation="MSLVETEKKDQVFIVRINRPERLNALGYEIRTLLAEAWNEFRES
                     KDLEIGIFTGTGRAFCAGEDMKESLEVGIVGGKRPTTEDPLMNGSLEKPVIAAVNGYA
                     MGGGFMLVEATDLRIAVPEAIFEVSEAKRWLLGGFNHGHIAKLPHPVATEMALGFRFT
                     AQRFHEIGFINKIVDSDELIDTAVSMAHHMLTLPPASRVNTIQMMREMKPRVSGSLEQ
                     LAAALKDHGDKNDLIESRKAFAEKRIPQFSGWDNPQDRYNPPTLTP"
     gene            1693..2832
                     /locus_tag="QF838_07130"
     CDS             1693..2832
                     /locus_tag="QF838_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60490.1"
                     /translation="MKITGLKINIFSSQHKNAKRNWLIVRIQTDEGVEGIGEASMLNQ
                     DPIVASLLEEWAGNYLIGKNPMNGELHWTRLHQDNLGRGGRLFSTALSGIDIALWDLR
                     GKILDVPVYELLGGPYAQKLRVYANGWYTTPGTPEQNAEEAKKVVAMGYTAMKFDPFG
                     KVAYTTITTEEAQLAEDRVAAVREAIGPNVDILVEVHARFNVHTAIHLGKRLEKYNPF
                     WFEEPVSQENVSEMKQVRSKIDIPVATGERLYLKFPFFDLVKNEAVDILQPDICNAGG
                     ITELKKIAGMAEAQHIMMAPHNTNSAVGTVASMHLDAAMPNFLIQEYHAEFYEPHYFE
                     VIEGLPRQENGYVDLPEGPGLGLTINEELMNAHPYLPLGMSERGI"
     gene            2829..3272
                     /locus_tag="QF838_07135"
     CDS             2829..3272
                     /locus_tag="QF838_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60491.1"
                     /translation="MNWLRRLMPQEVEQARESYNRWLQGFNSRDRDAMVAELHFPHLR
                     LSGSNQIQVWNTSADINANFDEQTQKLKEEGWVRTESNFIEAIQKGPDKVHLTMIQSR
                     IHSDGSEYNQFQTLWIFIREDDRWGVKFRSSFLVNATENTNFSTV"
     gene            complement(3291..4484)
                     /locus_tag="QF838_07140"
     CDS             complement(3291..4484)
                     /locus_tag="QF838_07140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO60492.1"
                     /translation="MSIELKTSTWDDFPLVKIINARGENGYRTQADFDAHQLVFTPER
                     SIMAFDGKHMVGNALSYEMDMYIPGGLSKIAAVASVSVQATHRRKGINRAIMKYQLED
                     IYSRGEPLAVLQASESIIYGRYGYGLASFESNLEIEKTRSAYAVEHASMGQTYFVEES
                     EAREIFPQIYSKAIENRNGMVTRQENWWEFRFREPGLKGGDPKSWFVKYQENGQDDGY
                     LRYTINGTELNVIELIASSHQAYSSLWRICLDMDLVDVIKAEHRPADDELKWMLADPR
                     RLVERSSDRYWVRLVDVRTALSQRSYSSDGSLILKIQDDFLPWNKQVVELSSESGKSL
                     CSISNKTPDIILTAGDLGAVYLGGINFSTLLSANRIEEVTEGSVAQANLMFSTSRKPW
                     GFDGW"
     gene            complement(4514..5302)
                     /locus_tag="QF838_07145"
     CDS             complement(4514..5302)
                     /locus_tag="QF838_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HJO60493.1"
                     /translation="MALDSKIITAISKELWDARVSGNSVTNPSQTYPEITTSDAYTIQ
                     RQLIDEALGKGEIIIGGKIGLSSKANQEKFGIAEPIYGHLFDSMILPEDEPIDISKMF
                     RPVVEPEICFLLDKELKGPGTNVGTVLSATKGVLPAFEIADSRLKKQSSRVEDNILDN
                     SGAAIVLLGGVLNPIEKIDLRLVGMVLERNGMVIDTGAGAAVLGHPAQAVAGLVNKLS
                     EFGMSLKAGSIIISGTPVAAVPAAKGDHFTASFDRLGTVSVNFI"
     gene            5498..6157
                     /locus_tag="QF838_07150"
     CDS             5498..6157
                     /locus_tag="QF838_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60494.1"
                     /translation="MGAISVNKLAGYSMMMGPVLALICYFITPGGLFIDTANPAAPGE
                     VVAALQGNATLSAITGILVPVGLIVFFSGLSQFVANMSGGTGHAIARLGTPMVLIAII
                     GWVVGSGISIGLSGATDPALGAGPVTVQIGITAISTLGTILFGLGAMLIACAASMRDE
                     NNTYMAYVASLAGVVAAVTGVIGGLSSGQLELMTMIGGICFLVFTIWSIIVGLAMSSS
                     D"
     gene            6318..6584
                     /locus_tag="QF838_07155"
     CDS             6318..6584
                     /locus_tag="QF838_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60495.1"
                     /translation="MEMFWEFTRKGQKLVLRVEDKQEMIGGVRETKNGFDAFAKTFTM
                     TPERAQKGLGSMEDAKDFVESFRPWELFLGPGDLQPESEVRETQ"
     gene            6600..7580
                     /locus_tag="QF838_07160"
     CDS             6600..7580
                     /locus_tag="QF838_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HJO60496.1"
                     /translation="MEKPVPKHRLVDSPLTVAALYHFALLDNLDQKRKGLLDICDKNE
                     ILGTFLLAEEGINGTVAGGSEAINLVIDYICSWNEVEKIDVKFSESSSRNFRRMKVKI
                     KKEIVTMGKDNIDPLNESGEYIEPHNWNEFISQDGLLLVDTRNNYEISMGKFKNAVNP
                     NTDSFRDFPSWADNLSNSGDKPEKIAMYCTGGIRCEKATAYMKQLGFEKVYHLKGGIL
                     KYLEEITETESMWEGECFVFDDRVSLTHGLNEGEYILCYGCQQPVSVDDCQSPLYEEG
                     VSCPKCYGELADSQRANSRERQKQIQLAYDRGEMHMGRKLPLKNRSKKLP"
     gene            complement(7601..>8476)
                     /locus_tag="QF838_07165"
     CDS             complement(7601..>8476)
                     /locus_tag="QF838_07165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJO60497.1"
                     /translation="NYRDVTVWECPPNGSGIAALMAMNIAEGFDIASMGSQSADRYHY
                     LIESLKLGYADALQYVADPRVTDVPIEGLLTKSYGSDRRKKISSDQAITDVSYGKPVP
                     NSDTVYLTVADKYGNACSFINSLYSGFGTGLVVPETGIALQNRGALFSLDPSHPNFLT
                     GNKRPYQTIIPAMATRNNEMWLSFGVMGGFQQPQGHLQVISNMVDFDMNSQQALDALR
                     FSVDVGETGAVRVEEGIDPKVVSELTKKGHQVEVITGYQRIMFGGAQTISRNPETGVL
                     VAGSEPRKDGAAVGY"
BASE COUNT         2347 a         1674 c         1874 g         2581 t
ORIGIN      
        1 gttaaggtga tttctaggtc agtcttggaa atgcaggctc gggaaaatag gccatcgttg
       61 gcaatatcat ctccatgtgt accatcgtca tgcagtttta caggaaaggt ttctatgttt
      121 cccgttgtga caattttcgt tgtttcatcg tcaaatattt tggatggaaa aaagcggaca
      181 acctccgata atttattctt gttcataaat gctaattctt cgcttgacag ttttggaatt
      241 aaggaacttg aaaaatcaca caatccgatc aatggagtaa aagcatagcg ttgattggca
      301 aatttggttg ttgctacagt ctcagtatta ataggagtca aggttgggat tggtgtgttg
      361 gttggagttg gagtactagc cggagttgta gttggcgtcg gaggcgttgt ggggtatgga
      421 gtgtaagtag gtagtggagt gtatgttggt actggagtgt aagtaggtag tggagtgtaa
      481 gtgggtatag gacttgggta agttaagatg agtgtggtag tggatggagg taactctgat
      541 tctatcccct catttttttc caagccaact tgtttttcag atttgatatc ttcatcgggt
      601 tttatattgt taccaagatt tatgatcccc ataactagaa gtggtgttag tactaccagt
      661 aggatggtta agacgatcat aactagtaaa ttaattttca taggaatatt ggtgccaaaa
      721 catatgacta atttgtcttt attctttgtt gtaaattaca actaattact ttatccctta
      781 ttttatggtg tcaaagttgg agggttatat ctatcttgag gattatccca accggaaaat
      841 tgggggattc tcttttctgc aaaggctttt ctggattcaa taagatcgtt tttatcacca
      901 tgatctttca aagctgcagc caattgttct agacttccag acacccttgg tttcatctcc
      961 ctcatcattt gtattgtatt aactctggat gcgggtggga gggtcagcat atggtgagcc
     1021 atggaaacag cagtatctat taattcatct gagtccacta tcttgtttat gaaaccgatt
     1081 tcgtggaatc tttgtgcagt aaatctgaat cccagagcca tttccgttgc aacaggatga
     1141 ggtaatttgg ctatatgccc atgattaaat cctcctaata accatctttt agcttcagaa
     1201 acctcaaata tagcctcagg tacagcaata cgaagatcgg ttgcttcgac tagcataaaa
     1261 ccacctccca tcgcataacc gtttacagct gcaattaccg gtttctcaag agacccattc
     1321 atgagaggat cttctgtggt cggtctttta ccgcccacta ttcccacttc cagcgattct
     1381 ttcatgtcct cccctgcgca aaaagctctt cctgtgcctg taaagatacc gatttccaaa
     1441 tccttcgatt ctcggaactc attccatgct tcggcaagca gcgtccttat ttcatagccg
     1501 agagcattta atcgttcagg acggttaatt cgaactatga aaacctggtc ttttttttct
     1561 gtttctacca atgacatctg tgtttcctcc gttgttccat tagtgagaag gctatttggt
     1621 gaatatacta acccttcacc cttgtaatac gatatgcacc attaattatg attttatctt
     1681 gtgggagcaa ttatgaaaat tactggatta aaaataaaca tattttcctc acaacataaa
     1741 aatgcaaaga gaaactggct aatagtacga atccaaactg atgagggagt agaaggaatt
     1801 ggtgaagctt caatgcttaa tcaagaccct atagttgctt ccttactcga agaatgggcc
     1861 gggaattatt taattgggaa aaacccaatg aatggtgagt tgcattggac tagactgcat
     1921 caggataact tggggagagg cggaagattg ttttcaacag ccttgtctgg aattgacata
     1981 gctttgtggg atttgcgtgg caaaatcttg gacgtgcctg tgtatgaact tcttggagga
     2041 ccttatgctc agaaattgag agtatatgca aatggttggt ataccactcc aggtactcct
     2101 gagcaaaatg ccgaagaagc gaaaaaggtc gtagctatgg gctatacagc catgaaattt
     2161 gacccatttg ggaaagtggc atacacgacc attacaacag aggaagcaca attagcggaa
     2221 gatagagtag ctgctgtaag agaagcaatt ggacctaatg tcgatattct tgtggaggtt
     2281 catgcccgct ttaatgtgca cacagccata catcttggga agagattgga aaaatacaat
     2341 cccttttggt ttgaggaacc cgtctcacag gaaaatgttt cagaaatgaa gcaggtccgg
     2401 tctaagatcg atataccagt tgccacagga gaacgacttt atctaaagtt cccgtttttt
     2461 gaccttgtta agaatgaagc tgtggatatt cttcagccgg atatctgtaa cgctggtgga
     2521 attaccgagc taaaaaaaat agctgggatg gcagaggcgc agcacattat gatggcaccg
     2581 cataacacta attcggctgt tggtactgtt gcgagtatgc atctagacgc ggcaatgcct
     2641 aattttttaa ttcaggaata ccacgcagaa ttttatgaac ctcattattt tgaagttatt
     2701 gaaggtttgc caagacagga aaacggatat gtggatttgc ctgaagggcc agggctaggg
     2761 ctaacaatta atgaggaatt aatgaacgca cacccttatt tacctcttgg tatgtctgag
     2821 agaggtatat gaattggtta aggagactta tgccacaaga ggtagagcaa gccagagaat
     2881 cttataatcg gtggttacag gggtttaatt caagggatag agatgcgatg gtagctgagt
     2941 tgcatttccc tcacctccgg ttgtctggat ctaatcaaat acaggtttgg aacacgtctg
     3001 cggatataaa cgccaacttt gatgaacaaa ctcaaaaatt aaaagaggaa ggatgggtta
     3061 gaacagaatc caatttcatc gaggcaattc aaaaggggcc tgacaaagta catctcacca
     3121 tgattcaatc cagaattcat tctgatggca gtgaatataa tcaatttcag actctgtgga
     3181 ttttcatacg tgaagacgat cgctgggggg ttaaatttag atcttctttt ttagttaatg
     3241 ctacagaaaa tactaatttt agtacggtct gacattaaat tacgatttgg ctaccaacca
     3301 tcaaaccccc aaggttttct cgaagtagag aacataagat tggcttgagc aacggagcct
     3361 tctgttactt cttcaattct atttgccgat aaaagcgttg aaaaatttat ccctcccaga
     3421 tagacagctc ccaaatctcc tgcagtcaat ataatatctg gggttttatt agagatgctg
     3481 cataaggatt tcccagattc ggaactcaat tccacaacct gtttattcca tggaagaaaa
     3541 tcatcctgta ttttgagtat taaagaccca tcagatgagt acgaccgttg agaaagggcg
     3601 gttctgacat ctaccagacg gacccagtac ctatcgcttg aacgctccac tagtcttcga
     3661 gggtctgcta gcatccactt caattcatca tctgcgggtc tgtgctctgc ctttatcacg
     3721 tcaacgagat ccatatccag acaaattctc caaagggatg aatatgcttg atgggaagac
     3781 gctattaatt cgataacatt tagttctgta ccattgatcg tataccgcag gtaaccatca
     3841 tcttgaccat tttcctgata tttcacgaac caagatttgg ggtctccacc tttcaaccca
     3901 ggttccctaa aacggaattc ccaccaattt tcctgcctag tcaccatccc attcctgttt
     3961 tctatggctt tcgagtatat ttgagggaat atttctcggg cttcagattc ttccacgaaa
     4021 taggtttgtc ccatagaagc gtgttcaact gcataggcac ttctagtttt ttctatctcg
     4081 aggttgcttt caaatgaagc aagaccgtac ccatatctcc cataaataat gctttcggat
     4141 gcttgtagaa cggcaagtgg ttctccccga gaatagatat cctcaagttg gtatttcatg
     4201 atagctcggt tgatgccttt tctacgatga gtcgcttgaa ctgaaacaga ggcaacggct
     4261 gctatttttg aaagacctcc aggtatgtac atatccattt cgtagcttag tgcgtttcca
     4321 accatatgtt tgccgtcaaa tgccataatg gatctttctg gtgtgaacac taattggtgt
     4381 gcgtcgaaat cagcctgggt tctatacccg ttctctcctc ttgcgttaat aatttttact
     4441 aaggggaagt catcccacgt tgaggttttt agttctatcg acatttaaaa tttcctaatt
     4501 acaccctgct tgattaaata aaattaactg atactgtacc tagtcggtca aaggaagccg
     4561 tgaaatgatc tccttttgcc gcagggacag cagcgaccgg ggtaccagat attattatgg
     4621 acccggcttt tagactcatt ccaaattctg acaatttgtt gactaatcct gctacagcct
     4681 gagcgggatg tccaagtaca gcagctccag cccctgtatc tatgaccatc ccgtttcgtt
     4741 ccagtaccat tccaacaagc ctaaggtcta ttttttcaat tgggtttaac acgccaccca
     4801 gaaggactat cgcggctccg gaattatcca aaatattgtc ttccaccctt gagctttgct
     4861 ttttcagtct agagtcggct atttcaaatg caggcagcac ccctttagtg gctgaaagaa
     4921 cagtgcctac attagtgcct ggccctttta gttccttatc aagtagaaaa catatctccg
     4981 gttctactac cggcctgaac atttttgaga tgtcgattgg ttcatcttca ggaagaatca
     5041 tagaatcaaa taaatggcca tagattggtt cggctattcc aaatttttcc tgatttgctt
     5101 ttgaactaag ccctattttg ccgccgatga ttatctctcc tttacctaag gcctcatcta
     5161 ttaattgtct ttgtatggtg taggcgtcag aagtagtaat ttcaggataa gtttgtgaag
     5221 ggttggttac agagtttcca ctgactcttg catcccataa ttcttttgat atagctgtaa
     5281 ttatttttga gtctaatgcc atcgtttacc tttccttgat gatatgtggt gattttaaac
     5341 tattctagtt aatctatttt tttttatatt tgtaaaaaaa cagaacaaga actacttggc
     5401 tgaaggacta ttgcaataga taatatattt atcagacagg gcaatagtcc tgtctgtttt
     5461 atttgaagtc cataatcacc aaatgaggag gattgaaatg ggcgcgattt cagtaaataa
     5521 gctggcaggg tattccatga tgatggggcc tgtccttgca ttgatttgtt acttcatcac
     5581 cccgggaggt ctgtttattg atacagcaaa ccccgcagca ccaggtgagg ttgtagcggc
     5641 ccttcagggt aacgctacat tatcggcgat aaccgggatt ctagttccgg taggactcat
     5701 tgtattcttt tcgggactct cgcagtttgt cgcgaatatg tccggaggaa ccggtcatgc
     5761 tattgctcgt ctaggaacgc ctatggtatt gatagcgata attgggtggg tggtaggttc
     5821 cggcatttct ataggacttt caggtgctac tgacccagcc ttgggtgctg gacctgttac
     5881 tgtgcagata ggtataaccg ctatcagtac tttaggtaca atcctgttcg ggttaggtgc
     5941 tatgttaatt gcttgcgcag cgtcaatgcg tgacgaaaac aatacctata tggcatacgt
     6001 cgcctctttg gctggtgttg ttgctgctgt aaccggagtt atcggtggcc ttagttcagg
     6061 ccagttagag ttgatgacta tgataggtgg gatttgcttt ttagtcttca caatatggag
     6121 tattatcgtc ggtcttgcta tgtcgtcttc tgactagttt gatttaagac gaatccagag
     6181 attttttaat ttccctttat tggggaagat ttcgttttta tcaaggtcaa accaatttgg
     6241 tttggccttg aagctttttc agatttctgt aacagggtaa actcatatat agattattta
     6301 aatttggagt acgctctatg gaaatgtttt gggaatttac gagaaaaggc caaaagttgg
     6361 tattgcgagt cgaagataag caagaaatga tcggcggggt tcgggaaacc aagaatggat
     6421 tcgatgcatt tgccaaaacc tttaccatga ctccagaacg tgcgcagaag gggttggggt
     6481 cgatggaaga tgccaaggat tttgtcgagt catttcgacc ctgggaatta tttctgggac
     6541 ccggtgattt acagccagaa tctgaagtca gagaaaccca gtaatattct ttattgatta
     6601 tggaaaaacc agtacctaaa catagacttg ttgactctcc tcttactgtg gctgctttat
     6661 atcattttgc tttgcttgat aatttggatc aaaaacgcaa agggctgtta gatatttgcg
     6721 ataagaatga gatattgggg accttcctac ttgcagaaga aggaataaac ggtaccgtgg
     6781 caggaggatc tgaggctata aacctagtca ttgattacat ttgctcatgg aatgaagtag
     6841 agaagataga cgtgaaattc tccgagagct cgtctaggaa tttccgtcgg atgaaagtga
     6901 aaatcaagaa agagatagtg acaatgggta aggacaatat tgatcctttg aatgaatcgg
     6961 gagaatatat tgaacctcat aattggaacg agtttattag ccaggacggc ttgttgttag
     7021 tagacacccg gaacaactat gaaatatcga tgggtaaatt taagaacgcc gtgaatccga
     7081 acacagacag ttttagagat tttccttcgt gggcagacaa tctttccaat tcaggagaca
     7141 aacctgaaaa aattgccatg tattgtacgg gtggtatccg atgtgagaaa gcgaccgctt
     7201 acatgaaaca attaggattt gaaaaagttt accatttgaa gggcggcatc cttaaatatc
     7261 tggaggaaat aactgaaact gaaagcatgt gggaagggga gtgttttgtc tttgatgata
     7321 gagtttctct gacccatgga ctcaatgagg gggagtacat tctctgctat ggatgccagc
     7381 agcccgtatc agtagatgat tgtcaatcgc cgttatatga agaaggggtg agctgcccaa
     7441 aatgttatgg ggaactggca gattcacaaa gagctaattc tagggaacgc caaaaacaga
     7501 ttcaattagc atatgatcgt ggcgagatgc acatgggaag aaagctgcca ctgaagaatc
     7561 gtagtaaaaa attaccttaa ttgtgataaa ttaatcctgc ttaatatccg accgcagcac
     7621 cgtcttttct gggttcggaa cctgccacaa gcactccggt ttcaggattc cggctgattg
     7681 tctgtgcgcc accaaacata attctctggt aacctgtgat tacttcgact tgatgacctt
     7741 tcttggtcag ttctgaaacc actttagggt caatgccctc ctcaacccta actgccccgg
     7801 tttccccaac atcgacactg aaccttagag catctaaagc ctgctgagaa ttcatatcga
     7861 aatcaaccat gtttgaaata acctgtaaat gcccctgtgg ctgttgaaag ccgcccataa
     7921 caccaaaact gagccacatt tcattatttc ttgtggccat ggcaggtatt atggtttgat
     7981 agggtcgttt attccccgtt aaaaagtttg gatgtgaagg gtccagagag aataaggccc
     8041 cacgattttg caaagctata ccagtttcgg ggactaccaa tcccgtgcca aatcctgaat
     8101 ataagctatt gataaaagaa caggcatttc catatttatc tgcaaccgtt aaatacactg
     8161 tgtctgagtt tggtactggc ttcccatatg agacatctgt tattgcttga tcggaactta
     8221 ttttttttct gcgatcactg ccgtaggatt ttgttaataa tccttctata gggacatctg
     8281 taactctggg atctgctacg tattgcaggg cgtcggcata tcctagcttg agagactcaa
     8341 ttaaatagtg atatcggtct gccgattggg aacccataga agctatgtca aatccctcag
     8401 cgatattcat cgccattagt gctgcgatac ctgaaccgtt tggcgggcat tcccacacgg
     8461 ttacatctct atagtt
//
LOCUS       DASCNS010000200         4644 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001553932, whole genome shotgun sequence.
ACCESSION   DASCNS010000200 DASCNS010000000
VERSION     DASCNS010000200.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001553932"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(50..379)
                     /locus_tag="QF838_07170"
                     /pseudo
     CDS             complement(50..379)
                     /locus_tag="QF838_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(608..683)
                     /locus_tag="QF838_07175"
     tRNA            complement(608..683)
                     /locus_tag="QF838_07175"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(648..650),aa:Lys,seq:ctt)
     gene            935..2827
                     /locus_tag="QF838_07180"
     CDS             935..2827
                     /locus_tag="QF838_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60498.1"
                     /translation="MSYFKFNSLGISALLIALSLAFAACTTEVEVIKEVEVIKEVPGE
                     TVTVTKEIVKEVPGETKTVTVTKEIEVSVPVAADLPAASKPKGKLTVAVPLVPPLVQL
                     QSLDARGTVGGFGIDWNIFEGIVRSQWAPPPTVPSQSTYVPEIASSWEIASDQSSITF
                     TIRDGIPWHEDRGDFGNVTAEDVVWTFNDSFKEGSTGNPGEQLPPGHKVGWEVVDGKA
                     QMNVGDNGFDPLWGMLHGGIGWGATYGITSKKAYDELGEDKFLTDAVGSGPYVVDAWD
                     GHDQMTARTSAGHWRASPRVETLQVIEMPEQGTREAAMLTGEVEIAQMPGKALPNLVE
                     NTGGKVITLGKPSPNTIYWSGNYWGETCPDCANTDVQANLEVDWPGAFEGLANGYAWI
                     GDPKDTASMENARQVRWALSMAIDRQKIVDTVLGGFGETAYVASNITPDRPEFNEEWR
                     VPYDVAGAKQKLIDAGYPEGFEITLWSACNLPAIWDCEIADAIGEMWITNLELDVTID
                     HADYAVRRPESVDKTMNTPWLHGIGWLPGQSIANFYCAHPGHLGGVTLPDDICAIGKQ
                     NDIEPDMDKRLGNNVAMTEYMSHWQTQSAVATVGNYFIYSPKIVEWRPYMTGYFNSAE
                     TIVLSE"
     gene            2911..3834
                     /locus_tag="QF838_07185"
     CDS             2911..3834
                     /locus_tag="QF838_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60499.1"
                     /translation="MGRFITRRLISLLVVVIAITLVVFMLSRATGDPRYLYMSSYTRM
                     TAEVWEAQGKAMGLDKPLIVQYLVWLKNAVQGDFGDSVHFRRNSLDLILKFLPATLQL
                     SGISFLCALLIGIPLGVLSAVKRTTGWDYLGRSFALLGQAAPPFWIALVFIWIFSVYL
                     GWLPTSRKGDWTHYVMPVITLGWLPAAGLLRLTRSSMLEVLDSEYVKLARAKGVSTKA
                     IIWKHAFRNSLIAPLTYAAILLANFLTGTVVVETVFAWPGLGRLSVMAALNTDFPLIT
                     GLALVFSVIFLLCSLVADILYALLDPRIRIS"
     gene            3849..>4644
                     /locus_tag="QF838_07190"
     CDS             3849..>4644
                     /locus_tag="QF838_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60500.1"
                     /translation="MVTLSSKRSLWGQVRSLPVLPVAIIVILLLVGIFAPLISPYPPL
                     EGNLDLRNIEPSWLSENNESPYFLGGDHIGRDVLTRIIYGTRISLIVAGTVLFFGTFI
                     GTLLGLMSGYLGGVVDEIIMRLVDFMYAIPFILVVLVAAVIWSPSLMLVIILLSIFTW
                     PPFARQVRAETLSLKQMDYVSLAIVSGTSPLRICLRHILPGLFNTVLVLASLQVGSLV
                     VTESVLSFLGVGIPAPQPSWGSMVAEGRNYMSTAWWICFFPGIAILL"
BASE COUNT         1197 a         1002 c         1116 g         1329 t
ORIGIN      
        1 tgacttcttc ttgatcattg ggatgcttcg ccattttcaa gccactcaac tacctaattt
       61 ttatcaattc aacctcaaag attaaagtgg tatttcccgg gataggacct acacctgcag
      121 caccatagcc gagctccggt gggactacca aacgccattt atcgccttct ttcatgattt
      181 ggagtgcctc ttgccagcca gcaataacac cattaaccgg aagatcgatc ggctcgccgc
      241 gctctactga actatcaaat acacgaccgt cttcaaaggt tccgtgatag tgagctgaca
      301 ctgtatccgt aggtccgggt gtagctccac cacctgctac taaaacttca tactgcagcc
      361 ctgaatcgtt tgtggtcata tccgccatat tatctacctc gctaatcatt cgatagtcaa
      421 tgaatctgtt gaatctgaag tctacagcga aaacgtggac tgaaacagat tacatcgatg
      481 aatctttaaa ggtatttttg aaaaagtacg tggattttat tccctccgtt ggagtaattc
      541 agctaagtgc atggtccggg acaggggaca tttagctaca gatttgttgt gttttgaagg
      601 aaggggctgg tgacccgcca cggacttgaa ccgtgaacct actgattaag agtcagttgc
      661 tctgccaatt gagctagcgg gccatgggtg ccgatatatt ttcacaattt cctaatgaac
      721 tagcaagggt ttgccatact caaaattttg cgcctaaatc aggctgcacc ttagtcggtc
      781 gggtattttt ttttggaata agcaaaatac gtatctagta ataatgttgc cacaacctag
      841 tcttatcata tagggttgta tgatcctatg atatcaacga ttggtaaatt attagcccgg
      901 tcccatgacg gttggaaaat gaagaggttt aattatgagt tattttaagt tcaatagtct
      961 tggcatttcg gctttactta ttgccttatc tttggctttt gcggcttgta ccacagaagt
     1021 tgaggtcata aaagaggtcg aggttattaa ggaagttcca ggtgaaactg ttacggtcac
     1081 caaggaaatc gtcaaagaag tccctggtga aactaagact gtaaccgtta caaaagaaat
     1141 agaggtttcg gtgcctgttg cggccgattt accagcagca agcaaaccta aaggtaaact
     1201 tacagttgca gtgcctttgg taccgccttt agttcaactg caatctttgg atgcaagagg
     1261 tactgtgggt gggtttggca ttgactggaa catatttgaa ggaatagtga ggtcacaatg
     1321 ggccccacct ccaacagttc cgtcgcagtc tacctatgta cctgaaattg cctccagttg
     1381 ggaaattgct agtgaccaaa gcagtatcac attcacgatt agagatggaa ttccttggca
     1441 tgaagacaga ggcgatttcg gtaatgtgac cgcagaagac gtggtgtgga ctttcaatga
     1501 ttccttcaag gaaggctcta ctggtaaccc aggtgagcaa ttacctcctg gccataaagt
     1561 gggctgggaa gttgtcgacg gaaaggccca aatgaatgtc ggtgacaatg ggtttgatcc
     1621 tctatggggt atgttgcatg ggggcatagg atggggggcc acttatggaa ttactagtaa
     1681 aaaagcatat gatgaattag gcgaagataa attcctcaca gatgctgttg gttccggccc
     1741 atacgtagtt gatgcatggg atggtcacga ccaaatgact gcgaggacat cggccggcca
     1801 ttggagagcg agcccacggg ttgaaactct acaagttata gaaatgcctg agcaaggcac
     1861 tagagaagct gccatgctta caggcgaggt agaaatagct cagatgcctg gtaaggctct
     1921 tccaaacctt gttgaaaaca ctggtggaaa agttattacc ttaggcaaac cttctcccaa
     1981 caccatctac tggtcaggaa actattgggg tgagacctgc ccggattgtg ccaacacaga
     2041 tgtgcaggca aacctggaag ttgactggcc tggagccttc gaagggctcg cgaatggtta
     2101 cgcttggatt ggtgatccca aagatacagc ctctatggag aatgctcgac aggtccgatg
     2161 ggccctttct atggctatag acagacagaa aatagtagat acggtcctcg gtggatttgg
     2221 tgaaacggcc tatgttgcaa gtaacatcac cccggataga cctgaattca atgaagagtg
     2281 gcgagtcccc tatgatgtcg ctggtgcaaa gcagaagctt atagatgctg gctacccaga
     2341 aggctttgag attactcttt ggtcggcctg caaccttcca gccatttggg actgtgagat
     2401 agcagatgcg atcggtgaga tgtggattac taaccttgag ttagatgtca ccattgacca
     2461 tgctgactat gccgttcgta gacctgaatc agttgataaa accatgaaca ctccgtggtt
     2521 gcacgggatc ggctggctac ccggacaatc aatcgccaac ttctactgtg cgcatccagg
     2581 ccaccttggt ggggtaacct tgccggatga catttgcgca attggtaagc aaaacgacat
     2641 agaaccagac atggataaac gactaggtaa taatgttgcc atgactgaat atatgtcaca
     2701 ctggcaaact caatcagctg ttgctacagt ggggaattac tttatttact cccctaagat
     2761 tgtggaatgg cgcccatata tgactggata cttcaatagt gctgaaacta tagtactgtc
     2821 agagtaacct gccagtctgg gacgagtaag aaactaagga gcgtccgcac ccctttcggt
     2881 gcgggcgctt cctcttttta ggaacaagat ttgggaagat tcataactag acgattgatc
     2941 tcactgcttg ttgtggtcat agcgatcaca ttggtagttt tcatgttgtc tagagcaact
     3001 ggtgaccctc gatatcttta tatgtcttca tatacccgca tgaccgccga agtatgggag
     3061 gcgcagggaa aggcaatggg tctcgacaaa ccgctgattg tccagtatct ggtttggttg
     3121 aaaaacgccg tccagggaga cttcggtgac tctgttcatt tcagacgtaa ttcactagat
     3181 ttgatattaa aattcctacc tgcaacgttg caattatcag gtatttcttt cttatgtgca
     3241 ttacttatag gaattccact gggcgtattg tccgctgtta aaagaacgac cgggtgggat
     3301 tacctgggac gaagttttgc tctccttggt caggccgcac ctcccttttg gatagcccta
     3361 gttttcatct ggattttcag tgtatatctc gggtggctcc cgacatctag aaaaggggat
     3421 tggacccact acgttatgcc cgtaataaca ctaggatggc ttccggcagc tggcctgtta
     3481 cgcttaactc gttcatctat gttggaggtg cttgatagcg aatatgtcaa attagcgaga
     3541 gctaaaggag tatcaacgaa agccataata tggaagcacg catttaggaa ttccttaata
     3601 gcccctttaa cttatgctgc tatattattg gcgaatttcc ttacgggaac tgtcgttgta
     3661 gaaaccgtct tcgcttggcc cggacttggc aggctatctg tgatggcagc cctcaacacg
     3721 gatttcccgt taattacagg tctggcactc gttttctctg tcatatttct actatgtagt
     3781 ctagtggcgg acattcttta tgcattatta gaccccagaa ttagaataag ttaaggaaga
     3841 ctaagcaagt ggtaacttta tcaagcaaac gcagcctttg gggacaagta agatctctgc
     3901 cggttttacc agttgccatc attgtaattc ttttactggt cggtattttc gcccctctca
     3961 tatcccccta ccctccttta gaaggaaatc tggatttgag aaacatagaa ccatcttggt
     4021 tgagcgagaa taatgaatct ccatactttc taggcggtga tcacattgga cgagacgtgc
     4081 tcacaaggat catttacggt actaggattt ctttgattgt tgctggtacg gttttgtttt
     4141 ttggtacgtt cataggtacg ttattaggcc ttatgtcagg ttacctcggt ggggttgttg
     4201 atgaaatcat aatgcgattg gttgatttca tgtacgcaat accgtttatt ttagttgtgt
     4261 tggtagcagc agtcatttgg tcccctagcc ttatgttggt aataatcttg ctttcaattt
     4321 ttacttggcc tccatttgcc agacaagtca gggcagaaac attatctttg aaacagatgg
     4381 actatgtttc tcttgccata gtatctggca cctccccact acggatttgc ttaagacata
     4441 tattgcccgg gctctttaac acggttctcg ttctggcctc tctgcaagtc ggttcactgg
     4501 tagtgactga atccgtctta agcttcttgg gagtaggtat accggcacct caaccatctt
     4561 ggggtagtat ggtggcagag ggtagaaatt acatgtctac cgcttggtgg atttgctttt
     4621 tccctggcat agctatcctc ctcg
//
LOCUS       DASCNS010000201        23523 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001554615, whole genome shotgun sequence.
ACCESSION   DASCNS010000201 DASCNS010000000
VERSION     DASCNS010000201.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 23523)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23523)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23523
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001554615"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(70..345)
                     /gene="rplF"
                     /locus_tag="QF838_07195"
                     /pseudo
     CDS             complement(70..345)
                     /gene="rplF"
                     /locus_tag="QF838_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
     gene            complement(357..758)
                     /gene="rpsH"
                     /locus_tag="QF838_07200"
     CDS             complement(357..758)
                     /gene="rpsH"
                     /locus_tag="QF838_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HJO60501.1"
                     /translation="MSVTDPIADMLTRIRNAVMVGHESVDVPASNLKVEIAQILEKEG
                     FLEGYEIQNEGQIDANIQLNLHYWQKGEPAITGLKRISKPGLRIYSKNDELPIVYGNR
                     GIAVISTNKGVMTGIEAKRSGVGGEVLFYVW"
     gene            complement(769..1074)
                     /gene="rpsN"
                     /locus_tag="QF838_07205"
     CDS             complement(769..1074)
                     /gene="rpsN"
                     /locus_tag="QF838_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HJO60502.1"
                     /translation="MAKKSSVARNEKRRRLAEKYAEKRRQLQNVIKDASSSSDARVEA
                     RIEMSKLPRNANPVRIRNRCQVTGRPHGYIRYFGLSRIAFREMAHAGELPGVKKASW"
     gene            complement(1114..1701)
                     /gene="rplE"
                     /locus_tag="QF838_07210"
     CDS             complement(1114..1701)
                     /gene="rplE"
                     /locus_tag="QF838_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HJO60503.1"
                     /translation="MPDDKTNQSATSSGSAPRLLQKIRDEIGPEMMKEFGYTSVMQIP
                     RLDKVVINIGLGEALQNARAIESVTADLGRITGQKPVTTKAKKSIAGFKIREGMPIGA
                     MVTMRGRRMYEFTDRLLNASLPRIRDFQGLSRNSFDGRGNYSIGIREHTIFPEIDYNS
                     IDRIRGMQIVIVTSAVNDQEGMRFLELAGMPFIRN"
     gene            complement(1701..2009)
                     /gene="rplX"
                     /locus_tag="QF838_07215"
     CDS             complement(1701..2009)
                     /gene="rplX"
                     /locus_tag="QF838_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HJO60504.1"
                     /translation="MKIKKNDNVLVIKGKDKGRQGPVQSVFPGKGKILVEGINIVKRH
                     RKAQGMSGAEIVEKELPVAVANVMLVCTNCQKPAKVSSRTMGDGSKGRICKNCEEVIE
                     "
     gene            complement(2013..2381)
                     /gene="rplN"
                     /locus_tag="QF838_07220"
     CDS             complement(2013..2381)
                     /gene="rplN"
                     /locus_tag="QF838_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HJO60505.1"
                     /translation="MIQIYTRLRVADNSGARVISCIGVPGGSGKKYAWLGDVIVASVK
                     DAAPAAGVKKGEVVKAVVVRTAKSHRRKDGSHIKFDENAAVIINEDKMPRGTRIFGPV
                     ARELRDNDFMRIVSLAPEVI"
     gene            complement(2399..2722)
                     /gene="rpsQ"
                     /locus_tag="QF838_07225"
     CDS             complement(2399..2722)
                     /gene="rpsQ"
                     /locus_tag="QF838_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HJO60506.1"
                     /translation="MSNDRRKIRVGRVVSQKMDKTVVVSVEWSQPHRIYKKNVKRWNK
                     FMVHDEGNSCNLGDTVRIVETRPLSKTKRWKVTEIIQKGDVAEVQPGEIAVPGVNEDN
                     GKDSE"
     gene            complement(2719..2925)
                     /gene="rpmC"
                     /locus_tag="QF838_07230"
     CDS             complement(2719..2925)
                     /gene="rpmC"
                     /locus_tag="QF838_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HJO60507.1"
                     /translation="MAEIDDIRNMNDQDLEENLDSMRKDLMNLRFRTATMQLTDTNEV
                     KRTQRKIARIMTVIREREISESSL"
     gene            complement(2976..3401)
                     /gene="rplP"
                     /locus_tag="QF838_07235"
     CDS             complement(2976..3401)
                     /gene="rplP"
                     /locus_tag="QF838_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HJO60508.1"
                     /translation="MLQPKRVKYRNHHRGRRKGLSVAGSSLNFGDFGLKATENAWVTS
                     RQIEAARRAMTRYARRGGRMWIRVFPDKSVTARAAETRMGKGKGAPDHWVAVVKPGKI
                     MFEMAGIPEEIAKHALTLAASKMPMGTRIVSRSNVRAKE"
     gene            complement(3388..4224)
                     /gene="rpsC"
                     /locus_tag="QF838_07240"
     CDS             complement(3388..4224)
                     /gene="rpsC"
                     /locus_tag="QF838_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HJO60509.1"
                     /translation="MGQKIHPNGCRVGITKDWQATWFAGQREYASLVKEDINIRQAID
                     AMYEDAGISKVDIERDSNEVAVTIHTARPGIVIGRGGQRVDELRKHLENLTGSRRAKL
                     NVVEVRQAELDAYLVARNVADQLERRIAFRRAIRQAVMRTMQAGAEGIKITASGRLAG
                     ADIARSEKAMEGRVPLHTLRADIDYGLAEAQTEYGVIGVKVWICRGEILNPTIIGENS
                     SSSDEIVASNEIEEADVAPIQVMVTAQDDNQVPTGSSLDQTQLTNEDNKEDEGANTDA
                     ST"
     gene            complement(4226..4564)
                     /gene="rplV"
                     /locus_tag="QF838_07245"
     CDS             complement(4226..4564)
                     /gene="rplV"
                     /locus_tag="QF838_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HJO60510.1"
                     /translation="MSIRAKSSNTGISVKKLKPIANLVRGMKVEEALVALQFMQSPAA
                     ARVAKTVKSAASNAENELMARSEDLKIIEIFANEGPRLKRFRARARGRVGRINRRSSH
                     ITVVVEEEEL"
     gene            complement(4593..4877)
                     /gene="rpsS"
                     /locus_tag="QF838_07250"
     CDS             complement(4593..4877)
                     /gene="rpsS"
                     /locus_tag="QF838_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HJO60511.1"
                     /translation="MSRSIKKGPYVHPKLAKKIDQAVKSNSREVIKTWSRASMIMPDM
                     LGLTIAVHDGRRHVPVFVTENMVGHKLGEFSPTRTFRGHSTKTERASRIG"
     gene            complement(4899..5732)
                     /gene="rplB"
                     /locus_tag="QF838_07255"
     CDS             complement(4899..5732)
                     /gene="rplB"
                     /locus_tag="QF838_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HJO60512.1"
                     /translation="MPLKTRKPTTPGQRGLVLQSTDDITERKPRKNLVRPLKKTGGRN
                     SSGRVTVRHRGGGHKRRYRVIDFKRDNYGVPGTVETIEYDPNRSCRIALIKFENGDWR
                     YILAPNEIHVGDPVRSGDDADIRPGHALPLEKIPSGTLVHNVELTKGKGGQIVRSAGG
                     YAQVLASEGKYTLLRLPSGEMRNVLSACMATVGQLSALDKKNVKLGKAGRKRNLGRRP
                     EVRGVAMAPNAHPHGGGEGRSPIGMPGPKTPWGKPALGYRTRKKKKNSSKFIVRRRYD
                     R"
     gene            complement(5732..6025)
                     /locus_tag="QF838_07260"
     CDS             complement(5732..6025)
                     /locus_tag="QF838_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HJO60513.1"
                     /translation="MSKHHKYAILKRPLITEKSTLMQEDGRYVFEVAKTATKLEVKEA
                     VQEVFGVTVINVNTMNVKGKMKRFGPKFSQKRSWKKAIVSVAPGDSITLFEGV"
     gene            complement(6039..6986)
                     /gene="rplD"
                     /locus_tag="QF838_07265"
     CDS             complement(6039..6986)
                     /gene="rplD"
                     /locus_tag="QF838_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HJO60514.1"
                     /translation="MKLQIKNIQGENAGDLEVRDDVFGVPVKSALVHQVMVGQLANKR
                     QGTAKTKTRSEVSGGGAKPRPQKGTGSSRQGSTSSPVWVGGGRAFGPSPRSYRKRTPK
                     KMRRLALLSVLSDKARHSDLLLLDSLELKEGKTKEIVSILSDLNISKSALIVTDGPNK
                     KLVQSAGNVGGVRTLPVQVLNTLELLNKKQLIITVDAVRRIEELWGGVYRGESPSSDS
                     SGEEIDVEKEEVHPEPPIVEDPVEEVAVEEVSITSVEDLNLSTRTRNILLQASVTEIS
                     DLIALSKVELMAIQSFGEKSYLEVREQLQNIKLLPSDWE"
     gene            complement(6992..7624)
                     /gene="rplC"
                     /locus_tag="QF838_07270"
     CDS             complement(6992..7624)
                     /gene="rplC"
                     /locus_tag="QF838_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HJO60515.1"
                     /translation="MSIVGIIGKVIGGTQYFYEDGTAHAVTAIEAGPCTITQIKSLKK
                     DGYQSVQLGFEKVKKSNRPKKGHLALAGGDLFRHLRETKSEDYENLELGSQINVEMFE
                     VGEKVNVTGVSKGKGFQGGVKRYNFRGGPKTHGQSDRHRAPGSIGAGTTPGRVIKGMR
                     MAGRMGGDKVTVKNLDVVMVDAERNLLLVKGAVPGHRNSVLLISKSNTKS"
     gene            complement(7635..7949)
                     /gene="rpsJ"
                     /locus_tag="QF838_07275"
     CDS             complement(7635..7949)
                     /gene="rpsJ"
                     /locus_tag="QF838_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HJO60516.1"
                     /translation="MVQNAQKIRVILKAFDHRILDQSAGQIVETAERTGARVAGPVPL
                     PTAIKRFCVLRAPHIDKDSREHFEMRTHNRLIDIHNPTSKTIDSLTNLDMPAGVDISI
                     KL"
     gene            complement(7962..10016)
                     /gene="fusA"
                     /locus_tag="QF838_07280"
     CDS             complement(7962..10016)
                     /gene="fusA"
                     /locus_tag="QF838_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HJO60517.1"
                     /translation="MAQKSLKQTRNIGFIAHIDAGKTTVSERVLYFSGRIHKLGTVDD
                     GNTATDWMDQERERGITIVSAATATQWKDWDLNIIDTPGHVDFTAEVERSLRVLDGGI
                     VVFDSVAGVQPQSETVWRQADKYQVPRICFINKMDKVGGDYYHAISTIVQRLKANPVP
                     IQIPMGSEDQFYGVIDLVEGRSFTFDDEGTMTEGGVPDEFAEEFEVRRSEMIEKIAEQ
                     DDNLLEKFLLEEDISEEELKAAIRSVTISNKVVPVLCGTALKNKCVQPLLDAIGEYLP
                     SPLDVPPVEGVKPGTDDVIARDANNDEPFSALAFKTVTDPFIGRLVYFRVYSGSAKSG
                     TSVLNTVTGDRERLGRIVKMHADQREDVEEVFAGEIAAAIGLKDTTTGETISSIQDPI
                     LLEKIEFPDPVVSVAIEPKARVDQDKLSDALVKLSDEDPTFTIVYDDETGQTVMSGMG
                     ELHLEILTDRMKREFKVEANVGKPRVAFRETIRKTTTAEGKFVRQSGGHGQYGHVIIE
                     MEPLERGEGIQFENRIRGGSIPTEFISSVEDGVRGALKNGPKSGFPVIDLLVRLIDGT
                     YHDVDSSKVSFEIAGSMATRTAIDRCGPVVLEPVMKLEVVTPEDYLGEVIGDLGRRRA
                     GVESIDAQGDTQIVKASLPLAESFGYANDLRSITQGRAGYSMEFEKYLEAPESVVEAQ
                     AT"
     gene            complement(10065..10535)
                     /gene="rpsG"
                     /locus_tag="QF838_07285"
     CDS             complement(10065..10535)
                     /gene="rpsG"
                     /locus_tag="QF838_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HJO60518.1"
                     /translation="MARRRRAEIRKILPDPKYNSVDLAKFINNVMSNGKKTVAQKIVY
                     SAIDNAEKEAGRSGFEVFEQAIRNATPMIEVRSRRVGGANYQVPAEVRPARRMALAMR
                     WLVTGARQRSGRGMSEKLSAELLDASRGQGAAVRRKEEVFRMAEANRAFAHYRW"
     gene            complement(10588..11004)
                     /gene="rpsL"
                     /locus_tag="QF838_07290"
     CDS             complement(10588..11004)
                     /gene="rpsL"
                     /locus_tag="QF838_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HJO60519.1"
                     /translation="MPTVNQLIRKKRRKKVRKNTAPALDLTWNTLKNKGNRGARSPHK
                     RGVCVQVRTQTPKKPNSALRKVARVRLTNGMEVTAYIPGEGHNLQEHSVVLIRGGKVR
                     DLPGVRYHVVRGVLDTAGVQDRKRSRSKYGTRIEEN"
     gene            complement(11195..11704)
                     /gene="rimM"
                     /locus_tag="QF838_07295"
     CDS             complement(11195..11704)
                     /gene="rimM"
                     /locus_tag="QF838_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HJO60520.1"
                     /translation="MDNDLVTVGIIRGIRGLRGQLRVESLSDFPERFSKGQFVQLDDL
                     PRIIGNVFNDKKGLIVELAGIESREAAEKYIGCALKIDSQESEDLNSENTYFHHEIIG
                     MVVLDQNNAELGAVTEIIETGANDVYVVSNSMDKDILIPAIGSVVGEVCVRDKTMRIT
                     LPEGLNTRS"
     gene            complement(11786..12016)
                     /locus_tag="QF838_07300"
     CDS             complement(11786..12016)
                     /locus_tag="QF838_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HJO60521.1"
                     /translation="MKELIEYIAKSIVSYPDDVVVSSSEKDDGDVAYILQVHPDDKGR
                     VIGRQGRVAQSIRSLLRVAAIKNGLHVSLEIE"
     gene            complement(12061..12330)
                     /gene="rpsP"
                     /locus_tag="QF838_07305"
     CDS             complement(12061..12330)
                     /gene="rpsP"
                     /locus_tag="QF838_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HJO60522.1"
                     /translation="MLRIRLRRTGSKNQPSYRIVVADSRKPRDGRFVEYLGHYNPRFD
                     PPEMVVDTEKAQKWISQGAQPSDAVKRIIEKVSSGNGESEDGAEA"
     gene            complement(12336..12599)
                     /gene="rpsT"
                     /locus_tag="QF838_07310"
     CDS             complement(12336..12599)
                     /gene="rpsT"
                     /locus_tag="QF838_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HJO60523.1"
                     /translation="MPSAKAFRVSERKKSINQPLRSRARNLVTRTRRMIADGDIEGAE
                     VAAKGAVVALDKAAGKGAMHRNTVSRKKSRLIKHLNDAKNNQE"
     gene            complement(12633..14855)
                     /locus_tag="QF838_07315"
     CDS             complement(12633..14855)
                     /locus_tag="QF838_07315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HJO60524.1"
                     /translation="MVNVGNVIVDDALYEKMGHYLASDKVELVAKAYAFAEKAHAGQS
                     RLSGEEFFEHPKQTALFLADLRLDANALSAALLHDVLEDCDVTFAELSMEFGSDVAGL
                     VDGVTKLTKAEVMGDSQFLPGSQSAFEEISLEDIAQAETLRKMLMAMAEDVRVVLIKL
                     ADRLHNMRTVGSLPQIRREAMAKETLEIYAPLAHRLGIWEIKWMLEDLSFQQIDPDAY
                     KQISLRLNSKRGKREEYIAKVVEIVKSELDKAGITADIYGRPKHIYSIHKKSERYHES
                     NREVSEIYDLFAVRILVNEVKDCYGTLGVIHTKWRPLPGQFDDYIANPKDNLYQSLHT
                     AVLCEESSPVEIQIRTYDMHQVAEYGVAAHWLYKETGELDVQFEEKMTWLRQLLEWQR
                     DVSGAVEFVESFKTDIFQNQVFVYTPNGEVIELPTGSTPLDFAYRIHTDIGHGCVGAK
                     VNGKLVALNYHLQNGDTIQIMTSKITRGPSLDWINPSLGYAKTSSARAKMRQWFNRQE
                     RQTNIERGKEVFQKQLARLDLDVTPEIVVEMFRYKAVDDMFADLGGGSLSTAKLISRI
                     SLKSELPDGLTRLTPPKKGPASGVQVLGVGDLLTNIARCCNPIMGDEITGFITRGRGV
                     TVHRKSCPNIISEDELDRIVPVDWGESRTLYPVRIQLDCWDRVGLLGDVTSVVSNERI
                     NIANINSEEYDDRCVISLTVYTNGIDQLGHLYTKLEAVNGVDGVTRLRNIDSRNDIEN
                     "
     gene            15055..16329
                     /gene="hisS"
                     /locus_tag="QF838_07320"
     CDS             15055..16329
                     /gene="hisS"
                     /locus_tag="QF838_07320"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HJO60525.1"
                     /translation="MEFQAPRGTTDVLPNDQRYWRYVENTASQVAESFGYRRIDTPVF
                     EDTNLYIRSIGDSTDIVEKETYTFSDRGGDSLTLRPEGTASVCRAYMQHGMHNMPQPV
                     RLFYLCPNFRYERPQAGRFRAFHQFGIEAFGESDPYIDAEVIEVSWRFLTSVGIDDIS
                     LRINSIGDSNCRPKYVDALQTYYQNYSQSLCPDCVRRLETNPLRLLDCKMESCQPVIQ
                     SAPKSTEYLCPPCNAHWVQLQEYLETTGIGYEIDNTLVRGLDYYSRTVYEITPPTERR
                     TNVIAGGGRYDGLLEQLGGPTTPGIGFGMGIERVIENIRRLEINIASNPKLSIMFAHI
                     GEGAKKEAISLSSQIRRLGKEAFVGPSRGLRSQMRYASHMNSSHVIIIGDDELSTGLY
                     TLRDLSKGDQTEITADGILNFVKTHDIGQKVD"
     gene            16330..17526
                     /locus_tag="QF838_07325"
     CDS             16330..17526
                     /locus_tag="QF838_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HJO60526.1"
                     /translation="MLQNDPSVFEMISQAQEYMQENEIDGWLLYDYRGMNPILWDAIG
                     YISNVTRPCWLWIPANSDPKFIVSFVDQNRFEQIGIKAEFWVSREQMIDSLKSHLPQH
                     GKVAMEYSPMGVLPRVAKIDGGTLELIRSLGVEVVTSANLIQYATQRWSDKELDSHLK
                     ASEILTTTVKEAFNFISENLDTNLTEFEVAEFIRNRFTKSNLITPDGPVVAINNHATD
                     PHFEPTEANSSTMSKGDWVLIDLWGRIGDEPSVCADITWTAFIGDEVPKKHLEVFNTV
                     IGGRDAAVTLLEKAYHEGRELQGWEVDAEARRYIDNMGYGKYFSHRLGHSLGREAHGN
                     AVNLDGWETIDTRTISPKIGFTIEPGIYIPGEFGVRSEIDLFYYETGPTVTTERQTSP
                     YLIQVS"
     gene            complement(17544..18809)
                     /locus_tag="QF838_07330"
     CDS             complement(17544..18809)
                     /locus_tag="QF838_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60527.1"
                     /translation="MESVEVSPELRKGRNQLAVTVTLGHAVKHVFNSALPILLTLMKG
                     DLGLSNTQYGVITSVGRGTSGATTMVAGYLGERFANRAGLMLFISLSMMGISYFLLGF
                     APSFWWVLAVMLLVGIGPSLYHPPAIASLSRKFPDRRGFAISLHGTGGSVGEMLGPIV
                     VGLLTTESVKTASIISLTYLVAFQWNEVLKISVFPALLFAVLVYLMMRNIPTVDSDTA
                     SLRDYFGDVGLLLKDKIMLGLIFVTAFRSMAQATIVAFLPVYLIDTLGKDAITTALFI
                     AGAQATGIVAQPIMGWLSDKYGRKIVLVPAMSLLGVLYLTLSVADDGVQLVLNVLAMG
                     AFVYSLHTIFIAAAMDVAKGRAQSTVVSLIYGASFLGTVGPVIAGLLSDTDLGIKSCF
                     IFSGVVALISTAILAGLKLPKTINQQQAH"
     gene            complement(18870..19700)
                     /gene="thiD"
                     /locus_tag="QF838_07335"
     CDS             complement(18870..19700)
                     /gene="thiD"
                     /locus_tag="QF838_07335"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HJO60528.1"
                     /translation="MTARMKTTPKCLTIAGSDSGAGAGIQADLKTFAANGVYGTSALT
                     AITAQNTTGVQDILELPVTLIENQIDSVLTDIGTDAVKTGMLSSSEIIRAVVGKLSQH
                     QIENVVVDPVMVAKGGDTLLQDEAIEALKTELIPIALVVTPNGPEATAITGINVVDVN
                     SAKAAAVKLVEMGCKTAVVKGGHINEGPATDIFYDGTSFHLFTTKRVNTLNTHGTGCT
                     FASAIAAGIAKGLSTRNSVSEAKAFVTGSIRHDLQIGNGHGPLNHFYEHWKSEESGIH
                     "
     gene            complement(19727..20386)
                     /locus_tag="QF838_07340"
     CDS             complement(19727..20386)
                     /locus_tag="QF838_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HJO60529.1"
                     /translation="MTLTSDLKAKYCGLWELVTYHRFVSQLGDGSLSPSIFKAYFLQD
                     YVFVNDLVVMAAQALAKAPNLKAASIFNDFLTGVLDAENDLFMRFFNQLGASEEDYSS
                     VEASPTTQAFGDFLVRTSLEGTFDEIVLVLYVTEGTYLDWGTRLREEKASLTNPVYRE
                     WIDLHGPEVLWDLVSWLENYINNDSSISAERADYLFHTALRYEFLFWESAYNDELWFD
                     V"
     gene            complement(20407..21387)
                     /locus_tag="QF838_07345"
     CDS             complement(20407..21387)
                     /locus_tag="QF838_07345"
                     /inference="COORDINATES: protein motif:HMM:NF020650.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60530.1"
                     /translation="MKNKLIGSILLTVLACLNFSCRPSTEIVQVKLALDWYPNANHIG
                     LYIAQEKGYFEDENLEVEIYTPSDPSTVLQTVASGADDFGMNYQPDVLIARSEGVPII
                     SILGMVQHPLNSVMALQSSGNKTPKDLKGKKVGYPGIPWNEDALNTMLESDGISGLDD
                     VELVNVGWELGSSMISETVDAIIGAYFTHESILLENEGHPVNVMRMEEWGVPDYYELV
                     MVTSENYLSENSDVVEKFTRAVGKGYADAISDPQSGVDILKKYSPEIDEDIDRPGADL
                     LQDLWKGKNGRFGTQEEARWVSFSNWMKVRGIINENLDPKAAFTDEFSAK"
     gene            complement(21420..22184)
                     /locus_tag="QF838_07350"
     CDS             complement(21420..22184)
                     /locus_tag="QF838_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60531.1"
                     /translation="MMINWLVKWSPAFGIVCLIVGAWQLLVKIQEIPSWLLPSPSSVA
                     TTIYNDHSMLMRHTIVTLEEIILGFFLALCVGVILASAICLSQTIEKALYPFIIASQT
                     VPIIVIAPMLLVWIGYGLAPKIIVVALISFFPIVVNTVDGMKSIDPDIQKLMHTLGGR
                     KAQIFSKIMVPSSLPYLFSGLRVAIAISVIGAVIGEWVGSSEGLGYLMIRSKPQFQTE
                     RVFAAIIILSFIGVGLFALITYLERVFVPWAHEKRN"
     gene            complement(22185..22958)
                     /locus_tag="QF838_07355"
     CDS             complement(22185..22958)
                     /locus_tag="QF838_07355"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO60532.1"
                     /translation="MEKIVDNHISIAGLEKTICQDGGPLKILDGVDLSIQKSEIISVL
                     GPSGCGKSTLLNIIAGLDEPDEGDINIDGLDFDSRLGSVGYMQQKDLLLPWRSVKDNV
                     ILGLEIKGVSKKKSYQMVEPYFQSFGLSGFESNYPHTLSGGMKQRASFLRTVMTEPDI
                     LLLDEPFSALDALNRSRLQLWLLDLLDGIQQTILLVTHDVDEAIMLSDRIYVMTSRPG
                     RINSVEKVPYSRPRNREIVNDSSFVKLKSKILSFLWDQD"
     regulatory      complement(23025..23139)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23191..>23523)
                     /locus_tag="QF838_07360"
     CDS             complement(23191..>23523)
                     /locus_tag="QF838_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60533.1"
                     /translation="LLATADSLSSSIPGLVAYSISQGGISVIPQSIIADYFGRKAFAT
                     ISGFRSSIQMLGIIVGPVVSGLVYDRTGSYEWAFLGFAAASIVSMLLVLMALPPSPRP
                     RDILETSQ"
BASE COUNT         6391 a         5869 c         4149 g         7114 t
ORIGIN      
        1 cgcaacccca gtacagaacg caacaccgga tccaccgcat caggccctaa aatcaccatc
       61 ccaaacccaa acacctacca attccaattc tttagtgaaa ccttcactca ctccttctac
      121 catatttgat agcaagctcc tggtgagtcc atgaaaggct ttatgctcgc tttcgtcact
      181 aggcctcgtg accttgacga tatctccttc ctggctaatc atcatatcct ggtgaaaatt
      241 ttgacttaag ctacccttgg ggccttttac tgtgacttcc gtcccgtcga tagttacatc
      301 gacacccgat ggtatttgta ttaattggtt tccaattctc gacataaaat ttctccctac
      361 cagacgtaga ataaaacctc accacctacc ccactccgtt tcgcttctat ccctgtcata
      421 acacctttat ttgttgagat cacagcaatt cctctattgc catatacaat cggtaattcg
      481 tcatttttgg agtatattcg aagacctggc ttgctgattc gctttaatcc agtaatagca
      541 ggttctcctt tttgccaata atggagattc aattgtatat ttgcatcaat ttgaccctca
      601 ttctgtatct catacccttc caaaaaccct tccttttcca atatctgagc tatctctacc
      661 tttaagttag aggctggcac atcaacagat tcatggccaa ccatcactgc gtttcgaatt
      721 cttgtgagca tatctgctat cgggtctgtt actgacattt tcttttacct accaacttgc
      781 cttttttaca ccaggtagtt cacctgcatg tgccatctca cgaaaagcta tacgagacaa
      841 cccaaaatat ctaatataac catgaggcct gccggttacc tggcaccgat ttcttattct
      901 caccgggtta gcgttcctag gcagttttga catctctatt ctagcctcca cgcgcgcgtc
      961 ggaagatgaa ctggcatcct ttataacatt ctgaagttgt cgtcttttct cagcatactt
     1021 ttctgctagc cttcgtctct tctcatttct tgcaacagat gatttcttcg ccacttttat
     1081 atccttagaa aattattaat cctattcaat tgcttagttt cttataaaag gcatgcccgc
     1141 caactctagg aatctcatgc cttcttgatc atttacagca gatgtaacta tcacaatttg
     1201 cataccacgt atcctatcaa tggagttgta atcaatttcc ggaaatatgg tgtgttctct
     1261 tattccaatt gagtaatttc ccctaccatc aaaagaattc cttgaaagac cttggaagtc
     1321 cctaattctt ggcaaggatg cattcaaaag cctatcggta aattcgtaca ttcttcgacc
     1381 cctcatggtc accatggcac ctataggcat tccttccctt atcttgaatc ctgctattga
     1441 tttcttagcc ttggttgtca caggtttttg gcctgtaata cggcccaaat cagccgttac
     1501 actttcgata gccctggcat tttgtaaggc ttctcctaac ccgatgttaa taaccacctt
     1561 gtccaaacga ggtatctgca tcacgctagt atagccaaat tccttcatca tctctggacc
     1621 tatctcatct ctaatctttt gcaaaagacg aggtgcacta ccacttgagg tggcagactg
     1681 attagtttta tcatctggca ttattcaata acctcttcgc aattcttgca gattcttccc
     1741 ttcgaaccgt ctcccattgt tctgcttgat acttttgccg gtttttggca gttggtacaa
     1801 acaagcatca cattagcaac cgctaccggg agttctttct ccacaatttc ggcaccactc
     1861 atgccttgag cttttctatg tcgtttaact atgttgatac cttcaactaa aatttttccc
     1921 ttacctggaa agacactttg gactggtcct tgtctacctt tatcctttcc cttaatcacc
     1981 aacacgttgt cgtttttctt tatcttcatt tcttatatga cctccggggc gagagaaacg
     2041 attctcatga aatcgttatc ccttaattct cgcgctacag gcccgaaaat gcgagtacct
     2101 cgaggcattt tgtcttcatt gatgatcacg gctgcatttt catcaaattt aatgtgtgat
     2161 ccgtcttttc ttcgatgaga ttttgcggtt ctcacaacca cagccttaac tacctcacct
     2221 tttttcactc ctgctgcagg cgcagcatct tttaccgaag cgactattac gtcacccaac
     2281 caagcatatt tttttccaga cccaccgggc acacctatac aactgatgac ccttgcgcca
     2341 gaattgtctg cgacccttaa tcttgtatat atttgtatca tcgctgtcgc ctttacattt
     2401 actcgctatc tttaccgtta tcttcattca ccccaggcac tgctatttct cctggctgaa
     2461 cctctgccac atcacctttt tgtattattt cggtcacttt ccatcttttg gttttagaca
     2521 aaggcctagt ttctacaatc cgaaccgtgt ctccaagatt gcaagagttc ccctcgtcgt
     2581 gaaccataaa tttattccaa cgcttaacat ttttcttata tattcggtgg ggctgactcc
     2641 attcaaccga gacgacaacg gttttatcca tcttctggct gactactcgc cctaccctta
     2701 tttttctacg atcgttactc ataaggaact ctctgatatc tctctttctc ttataactgt
     2761 cattatcctt gctatcttcc tttgagttct tttcacttcg ttagtatcag taagttgcat
     2821 cgttgcagtt ctaaatctca aattcataag atctttccgc atagagtcaa gattttcttc
     2881 taaatcttgg tcattcatat ttctgatgtc gtcaatctcc gccataatta gctcactgtt
     2941 gtggtttgga atttttcatt ggtttgttga ctactctact ctttggccct cacattagac
     3001 ctagaaacaa ttcgggttcc catgggcatt ttggatgcag ccaaagtaag tgcatgttta
     3061 gcaatttcct ccggtatacc ggccatctca aacattattt tccctggttt aacaactgca
     3121 acccaatgat ctggtgctcc tttacctttt cccatcctag tctcagcagc cctagccgtt
     3181 acacttttgt caggaaacac tcttatccac attcttccac ccctcctggc atacctagtc
     3241 atagctcttc tggcagcctc aatttgtcta gaagttaccc aagcgttttc agtggcctta
     3301 agtccaaaat caccgaaatt taaggaacta ccagcgacag ataatccttt cctcctaccc
     3361 ctatggtggt ttctatattt cactctttta ggttgaagca tctgtgtttg ctccctcatc
     3421 ttccttgttg tcttcgttag tcaattgcgt ttgatccaat gaagatcctg taggaacttg
     3481 gttatcatct tgagccgtaa ccatcacctg gattggtgcc acatctgctt cttctatttc
     3541 gtttgaagca acaatttcgt ccgaactaga tgaattttcc ccaattatcg tggggtttaa
     3601 tatttcccct ctacaaatcc atacttttac cccaataaca ccatactccg tttgagcctc
     3661 ggctaatcca taatctatgt ctgcccttag ggtgtgtagt ggaacccttc cttccatcgc
     3721 tttctccgac ctagctatat ctgcgccagc aagtcttcct gaggctgtga tttttattcc
     3781 ctcagcccca gcctgcatcg ttcgcatcac tgcttgtcga atagctcttc tgaaagctat
     3841 tctcctttca agttggtcag ccacgttgcg tgctacaaga taggcatcta attctgcttg
     3901 cctaacctct actacattaa gtttggctct tcggcttcct gtgaggtttt ccagatgttt
     3961 cctaagctca tctaccctct gacctcctct accaatcaca ataccgggtc ttgcggtatg
     4021 gatggtgact gccacctcat tagaatccct ttcgatatca acctttgata ttcctgcatc
     4081 ttcatacatc gcatcgattg cttgccgaat attaatatcc tctttcacaa ggctggcgta
     4141 ctctctttgc cctgcaaacc aagtagcctg ccaatcttta gtgataccta ctctacaacc
     4201 attagggtga attttctgtc ccaaactaca gttcctcctc ttcaactaca actgttatat
     4261 ggctacttct tctgttaata cgcccaactc ttccccgtgc ccgagctcta aaccttttga
     4321 gtctagggcc ttcattagcg aatatttcaa ttatttttag gtcctcgctt ctagccatca
     4381 attcattttc tgcatttgac gccgctgatt ttacagtttt cgcaactcta gctgctgctg
     4441 gagattgcat aaattgcaag gctaccaagg cctcttcgac cttcataccc ctaactaaat
     4501 tagctatagg cttcagcttt tttaccgata tccctgtatt actcgatttt gctcttatac
     4561 tcactactgt tcctcagtaa acgttaatac ttttatccga tcctagacgc tctttccgtt
     4621 ttagttgaat gacctctaaa agttcgagtt ggtgagaact ctcctaattt atgtccaacc
     4681 atattttcag tgacaaatac cggcacgtgc ctcctgccat catgaactgc aatagtcaat
     4741 cccagcatat caggcatgat catagaggct ctggaccatg tttttatcac ttctctacta
     4801 tttgatttaa ctgcttggtc aatttttttt gccaactttg ggtgcacgta cggccctttc
     4861 tttattgaac gcgacattta gctacacctc tcgataattt acctgtcata tcttcttctt
     4921 actatgaatt tgctggaatt ctttttcttt ttcctagtcc ggtatccaag cgcaggttta
     4981 ccccatgggg tttttggccc aggcatacct atcggagacc taccttcacc gcccccgtgt
     5041 ggatgagcgt ttggagccat tgcaacccct cgcacctctg gccttctacc caagttcctc
     5101 ttcctgccag ctttcccaag tttcacattt ttcttgtcta gggcactcaa ttgaccaacc
     5161 gttgccatgc acgcacttaa aacatttctc atctctccgg acggtaatcg cagtaaggtg
     5221 tacttcccct cactagccaa tacctgtgca tagccacccg cacttctcac aatctgcccg
     5281 cctttccctt tggtcaattc gacattgtgt accaaggttc ctgaaggtat tttttctaat
     5341 ggcaatgcat ggcctgggcg aatatcagca tcatcaccag acctaactgg gtctcctacg
     5401 tgaatttcat ttggggccaa aatatatcgc caatcaccgt tctcaaattt aataagcgct
     5461 attcggcagg aacggttagg atcgtattca atagtctcaa cggtccccgg cacaccataa
     5521 ttatctcttt taaaatcaat caccctatac cgtcttttat gaccaccacc acgatgtctt
     5581 actgttacac gtccgctgga atttcttcca cctgttttct ttaaaggtct aactaagttt
     5641 tttcgcggct ttctttccgt tatatcgtca gtactctgga gtactaaccc cctttggccc
     5701 ggggttgttg gttttctagt ttttagtggc attaaactcc ctcaaacaaa gttatggaat
     5761 ccccaggggc cacagaaaca atcgcctttt tccaggagcg tttttgggag aatttgggac
     5821 caaagcgttt catttttcct ttcacgttca tggtgttgac gttaattacc gtgactccga
     5881 aaacttcctg gactgcctct ttgacttcca gtttagtagc agttttggcg acctcgaata
     5941 cataacgacc atcttcctgc atcagtgtag atttttcagt aatcaagggt cgttttagaa
     6001 tcgcgtattt atggtgctta ctcatctctg cttacctttt attcccagtc tgagggcagt
     6061 aactttatat tttgcagctg ttcccgaact tccagataag acttctctcc aaaactttgt
     6121 atagccataa gttccacttt ggatagagcg atcaggtcac tgatttctgt aacgcttgct
     6181 tgcagcaata tatttctagt tctggttgaa agattgagat cttctaccga agttatactg
     6241 acttcttcta cagcgacttc ttccaccgga tcttcgacaa ttggaggttc aggatgaact
     6301 tcttctttct ccacatcaat ttcttcacct gaagaatcgc ttgaagggct ttcccctcta
     6361 tatactcccc cccataactc ttcgatcctt cttactgcat caactgtaat tattaattgc
     6421 tttttattaa gtaactctaa tgtgttgagt acttgcactg gtaaggttct tactccacca
     6481 acatttcctg ctgattgaac caatttctta tttggtccat cagtcactat caaagctgat
     6541 ttcgagatgt tcaaatcaga aagtatcgac acaatttctt tcgtctttcc ttccttcaat
     6601 tctagtgaat caagaagaag taaatctgaa tggcgggcct tatcagacaa aactgacagc
     6661 aatgccaaac gcctcatctt cttcggagtc cgtttcctgt agcttcgcgg ggaaggacca
     6721 aaggctcttc caccaccaac ccacacaggg gaactagtag agccctgccg cgaacttcct
     6781 gtgccttttt gtggtcgagg tttggctccg ccgcctgaca cttctgatct agttttggtt
     6841 ttggctgtgc cttgcctctt gttggccaac tggccaacca ttacctgatg aaccaaagcc
     6901 gatttaaccg gcaccccaaa tacatcatca cgaacttcca aatcgccagc attctctccc
     6961 tgaatatttt ttatttgaag tttcactact tttaactctt tgtattcgat ttagatataa
     7021 gtaacacaga attacgatgc cccggtacag cacctttcac caacagaaga ttcctctcgg
     7081 catctaccat tacaacatcc aaatttttca cggtcacctt gtctccaccc attcgacccg
     7141 ccattctcat tcctttaatg acacgccccg gagttgtccc agccccaatt gatcctggag
     7201 ctctatgcct atcagactga ccgtgagttt tgggtcctcc tcgaaagtta tatcttttga
     7261 cgcccccctg aaaacctttt ccctttgaaa cccctgtgac gttaactttt tcccccacct
     7321 caaacatttc cacattaatt tgagacccga gctctaaatt ttcatagtct tcagactttg
     7381 tctctctgag gtgccggaat aggtcaccac ctgccagcgc caaatgaccc ttctttggtc
     7441 tatttgactt tttaactttc tcaaatccga gttgaacgct ttggtatcca tcttttttta
     7501 aggatttgat ctgtgtgatg gtacaaggac cggcttcaat agctgtaact gcgtgagctg
     7561 taccgtcctc ataaaaatac tgggttcccc caatgacctt acctattatc cctacaattg
     7621 acatctttct gtacctacaa tttgattgat atgtccacac cagcaggcat gtccaaatta
     7681 gtcaatgaat ctatcgtttt agatgtaggg ttatgtatat cgataagtcg attatgggtc
     7741 cgcatctcaa aatgttcccg agaatcttta tcaatatggg gcgcccgtaa cacacaaaac
     7801 cgtttaatag cagtgggtag tggcaccgga ccggcaaccc tagccccagt cctttccgcc
     7861 gtttcaacta tttgacctgc ggactgatcc aaaattctat gatcaaatgc tttcaatata
     7921 actctgattt tttgggcgtt ttgtaccatt tcttaaatgt cctaagtggc ctgagcttct
     7981 acaactgact caggagcttc caaatatttt tcgaattcca tagaataccc tgcccttcct
     8041 tgagtgatag agcgtaaatc atttgcgtat ccaaagctct cagccaacgg aagagatgct
     8101 ttaacaattt gagtgtcccc ttgagcatcg atactctcca ccccagccct tctcctaccc
     8161 aagtctccga ttacttctcc caaataatct tcaggggtaa caacttccaa tttcatcaca
     8221 ggctccaaaa cgactgggcc gcacctgtca atagctgttc tggtcgccat tgagccagca
     8281 atttcaaaag aaactttgga tgaatcaaca tcgtgataag taccatcaat aagcctgact
     8341 agaagatcta ttactgggaa tcccgactta ggcccatttt tcaacgcacc cctaacacca
     8401 tcttccactg aggatataaa ttccgtcgga atagatcctc ccctgatacg gttttcaaac
     8461 tgtatcccct cgcctctttc cagtggctcc atctcaatga tgacgtgccc gtactgacca
     8521 tgacctccgg attgccttac aaattttcct tcagcagttg tagtttttct aatagtctcc
     8581 ctgaatgcaa ctctaggttt tccaacattg gcttcgactt taaattctct cttcatacgg
     8641 tcagtcaaaa tttcaaggtg caattcaccc atacctgaca taacggtctg cccggtttcg
     8701 tcatcataaa caattgtgaa agtaggatct tcatcagata atttgaccaa tgcatcagac
     8761 aacttatcct gatctaccct agctttgggt tcaattgcaa cagaaacaac cggatctgga
     8821 aattcaatct tttctaataa gattgggtct tgtatcgagc tgatggtctc accagtcgta
     8881 gtgtctttca aaccaattgc tgcagcaatt tcccctgcaa aaacttcttc gacatcttcc
     8941 ctttgatctg cgtgcatttt aactattctg ccgagccgct ccctgtctcc tgtcacagta
     9001 ttcaaaacac tcgtccctga cttagctgaa ccggaataga cacggaaata aacaagccta
     9061 ccgataaagg ggtccgtcac agttttgaaa gcaagcgcgg agaacggttc atcgttgttg
     9121 gcgtctcttg cgatcacatc atcagtccct ggcttcacac cttcaacagg gggaacatcc
     9181 aaaggtgaag gtaaatattc tccaattgca tctaacagag gctgaacaca cttgtttttt
     9241 agggctgttc cacaaagaac aggaacaacc ttgttagaaa tggttacact ccgtatagct
     9301 gctttaagtt cttcctccga aatatcctct tcaagaagaa atttttccag cagattatcg
     9361 tcttgttctg ctatcttctc aatcatttct gatcgtctaa cctcaaattc ttcggcgaac
     9421 tcatctggga ctccaccttc cgtcatcgtg ccctcatcat cgaatgtgaa agatctacct
     9481 tccacaagat caatgacccc ataaaattga tcctcacttc ccatcggtat ctgtatcggg
     9541 acagggttcg cctttaacct ctgcactatg gtgctaatcg cgtggtagta atcaccaccc
     9601 accttatcca ttttatttat aaaacaaatg cgaggcactt gatatttatc agcttgtctc
     9661 cacacagtct cggactgagg ttgaactcca gctacggaat caaacacaac aatgccaccg
     9721 tccaaaaccc taaggctcct ttctacttca gcggtaaaat ccacgtgtcc aggggtatca
     9781 ataatattta aatcccaatc tttccattgc gttgctgtag cggcagaaac tatggttatt
     9841 cccctctctc tttcttggtc catccaatct gtagctgtat ttccgtcatc aaccgtaccc
     9901 aatttgtgga tgcggccgga aaaatataaa actctctctg acacagttgt tttacctgcg
     9961 tcaatatggg caataaaacc aatatttcta gtttgtttta gggacttttg tgccactgtc
    10021 tgtatccttc attaggcatt attcaatagg gattagtaaa tcgatcacca gcgataatgc
    10081 gcaaaggccc tgttagcctc cgccatcctg aaaacctctt ctttacgcct cacagctgct
    10141 ccctgtcccc tagaggcatc caataattcg gctgacaact tctctgacat tcccctacct
    10201 gacctttgcc tagcaccagt aacaagccat ctcatggcca aagccatacg ccgtgccggc
    10261 cttacttcgg cgggcacttg gtagttggct cccccaactc ttctagaacg aacctcaatc
    10321 ataggagtag catttcgaat tgcttgctcg aaaacctcga atccagatct gcctgcctct
    10381 ttctctgcat tatctatggc agagtaaaca attttttggg caactgtttt tttaccattg
    10441 gacatgacat tatttataaa cttggctaga tccacactat tatatttagg atccggcaaa
    10501 atctttctta tttccgctct tcttcttctc gccatataac ttctctgaac tgatacctta
    10561 atttacgata agtgtctatc aatcaactta attttcttct attcgagttc cgtacttact
    10621 acgacttctc ttccgatcct gtactccagc tgtgtctaaa acccctcgta cgacgtgata
    10681 ccgtacccct ggcaaatccc gaaccttacc ccctcttatc aaaacaacgg agtgttcctg
    10741 caaattatgt ccctctcccg ggatataggc ggtgacctcc attccgttgg tcagtcttac
    10801 ccgagccacc ttccttaggg ccgaattcgg tttcttaggt gtctgagtcc tcacttgcac
    10861 acataccccc cgtttatgtg gagagcgtgc ccctcggtta cccttattct tcagagtatt
    10921 ccaggtgaga tctaacgcag gtgccgtatt tttccgtacc tttttgcgcc tcttcttcct
    10981 aataagttga ttaaccgtag gcatataatt tcctgttttt ttgtctcttt ttttgaccga
    11041 aaacagccac cgcttttagg ggcgacaagg aataattata tctttggaca aaatgtgtgt
    11101 caacgattac aaagtctaaa atttacgaac aaattagagt ctgaggtgcg ttcgtaaatc
    11161 aataccaaat aacttgaatc gtgtaactct aagttcagct cctagtattt aagccctcag
    11221 ggagagtaat tctcattgtt ttatctctca cacatacctc acccacaact gagccaatcg
    11281 ctggtattaa aatatcttta tccattgagt tacttacaac gtaaacgtca ttagctcctg
    11341 tttctataat ctctgtcacc gctcccaatt ccgcattgtt ttggtccaaa actaccatcc
    11401 caataatctc atgatggaaa taggtgtttt ctgaatttaa gtcttcagat tcttgagagt
    11461 caatcttcaa tgcacatcct atatatttct cagcagcctc tcggctttct ataccagcta
    11521 actctacaat caatcctttt ttatcattaa aaacattacc tattatcctc ggtagatcat
    11581 caagttgtac gaactgacct tttgaaaatc tctctggaaa atcagataga gactcgactc
    11641 gtaattgacc tcggaggcct cggatacccc gtattatccc gactgtaact aagtcattat
    11701 ccatataaaa ccaaccatgc gaatattttt gaaggtgttg tgacccaact ctactgatct
    11761 ttggggaaca tggaacgtga ggtgtttatt cgatctccag actgacatgc aatccgttct
    11821 tgattgctgc tacccgcaac aagcttctta tcgattgggc tactctacct tgtcgtccaa
    11881 tcactcgacc tttgtcatca ggatgaacct gcaatatata agcgacgtct ccgtcatctt
    11941 tctcacttga ggaaactacg acgtcatctg gatacgacac aatagattta gctatgtact
    12001 ctataagttc tttcatatta actagaaatg gggagaaatt taaattgcat tcgagaaaat
    12061 ttacgcttct gccccgtctt ctgattctcc attgccactt gaaacctttt caattattct
    12121 ttttacggca tcggagggct gagctccctg actaatccat ttctgtgcct tttcagtatc
    12181 aacaaccatt tccggaggat caaaacgggg attatagtgc cccaggtatt cgacaaagcg
    12241 cccatctctc ggcttgcgag aatctgctac aactattcga taacttggtt ggtttttgga
    12301 gcctgtcctt ctaagcctaa ttctaagcat gacagttact cctgattatt cttagcatcg
    12361 ttgagatgct tgataagtct tgatttcttt ctagacaccg tatttctatg catcgcaccc
    12421 ttcccagcag ctttatccaa ggccactact gccccctttg cagctacttc agcaccctct
    12481 atgtccccat cggcaatcat cctccgcgtc ctcgtaacca aatttctggc ccgtgatctt
    12541 aagggttggt tgatactctt ctttctttca ctaaccctaa aagcttttgc gcttggcact
    12601 ttaatcgagt ctcctattgc attttctaaa aactaatttt caatatcatt tcgggaatca
    12661 atattcctca gccgggtaac cccatcaaca ccattaacag cctctaattt cgtgtacaga
    12721 tgtccaagct gatcaatccc atttgtgtat accgtcagcg aaatgacgca cctatcatcg
    12781 tactcttcag aattaatatt ggcaatatta attcgttcat tagataccac tgacgtaacg
    12841 tctcccaaca atccgacacg gtcccaacaa tccaattgaa ttctcacagg gtacaaagtc
    12901 ctggattctc cccaatccac tggtactatc ctatccagtt catcttctga gattatgttt
    12961 gggcacgatt ttctatgtac agtgacacct cgtccccggg taataaaacc ggtgatttca
    13021 tcccccataa ttggattaca gcatctagca atattggtta acaaatctcc tacaccaaga
    13081 acctgcaccc ctgaagctgg tcccttttta gggggtgtca atcgggtaag cccatcagga
    13141 agttcagact tcaacgatat tctagatatc aatttagctg ttgataaaga accgccaccc
    13201 aaatcggcaa acatatcatc aacagctttg tacctaaaca tttccactac tatttccgga
    13261 gttacgtcca aatccaatct tgctaattgt ttctggaata cttctttccc acgctctata
    13321 tttgtttggc gttcttgtcg gttaaaccat tgacgcatct tggcacgtgc tgaagaagtt
    13381 ttagcatatc ctagactagg gttaatccaa tctaagcttg gtcccctagt gatcttgctg
    13441 gtcattatct gaattgtgtc accgttttgt agatgataat tcagagccac caatttgcca
    13501 ttcactttcg ctccaacaca accatgccca atatccgtat gaattctgta agcaaaatca
    13561 agtggggtag aaccggtggg aagttctata acctccccat taggtgtata aacaaaaacc
    13621 tgattctgaa aaatatccgt tttgaaagat tctacaaatt ctactgctcc actcacatca
    13681 cgttgccatt ctaaaagttg ccgcaaccaa gtcattttct cttcaaactg aacatcaagt
    13741 tccccagttt ctttgtagag ccagtgggct gccaccccat attcggctac ctgatgcata
    13801 tcatacgttc gaatttgaat ttccactggg gatgattctt cgcacaaaac ggcagtgtgg
    13861 agagactggt aaagattgtc tttaggattg gctatatagt catcgaactg tccaggcagg
    13921 ggcctccatt tagtatgaat tactccaaga gtgccgtaac agtctttcac ttcatttact
    13981 aggatacgta cagcaaaaag gtcatatatc tcactgacct ctctgttgga ttcatgatac
    14041 ctttctgact tcttgtgaat gctatatatg tgtttaggcc ttccatatat atccgctgtt
    14101 atgccagctt tgtctagttc agattttact atttctacca ctttggcaat atactcttcc
    14161 cgcttaccac gtttggaatt caaccgaagg gagatctgct tatatgcatc aggatctatt
    14221 tgctggaaag ataaatcctc aagcatccat tttatttccc aaatgcccaa cctatgagcc
    14281 aagggagcat atatttccaa agtctctttg gccattgcct ctcggcgaat ttgaggtagt
    14341 gacccaacag tccgcatgtt atgtagccta tcggctagct tgattaagac caccctcaca
    14401 tcctcagcca tcgccataag catttttctc agggtttctg cttgagcaat gtcctctaat
    14461 gaaatctcct cgaaagcact ctgggatcca ggcaaaaact gggaatctcc cattacctcc
    14521 gccttggtaa gctttgtaac tccatccact aatcctgcaa cgtcactccc aaattccatg
    14581 gaaagttcag caaacgttac atcacagtct tccaaaacat cgtgaagtag agcggcggag
    14641 agagcgtttg catccaagcg aagatcagcc aaaaaaaggg ccgtctgttt tggatgttcg
    14701 aaaaattcct ctccggaaag tcggctctga ccagcgtgcg ctttttcggc aaaagcatag
    14761 gccttagcca ctaactctac cttgtcagaa gcaaggtaat gccccatttt ttcatataag
    14821 gcatcatcga caattacatt cccgacatta accatggagc gaaactctcc aaactattgg
    14881 tcaccaaaca aaccaagaca ttacttggct tgctcctctc gttccccatt tgtctctact
    14941 gaaacatatt ttgaaagttg gcaaattata tcgcaacttg agcagtatga cgaagccact
    15001 ctgatcttaa tttttaatat aaaatggttg ggttccaaaa tagagggaga tgaaatggaa
    15061 ttccaagccc cacgaggtac cactgatgta ttaccaaatg atcaaagata ttggcgctac
    15121 gtagaaaaca ccgcttcaca ggttgctgaa agcttcggat atagaagaat tgatacacct
    15181 gtttttgaag ataccaatct gtatattaga agcattggtg attcaacaga catagtggaa
    15241 aaggaaacct acacctttag tgatagagga ggggattcac taacccttag accggagggt
    15301 acagcttctg tttgtcgagc ctacatgcaa cacggtatgc ataacatgcc ccaaccagtg
    15361 agacttttct atctttgtcc aaatttcaga tacgaaagac ctcaggccgg caggttcaga
    15421 gcctttcacc aatttggcat agaggccttt ggcgaatcag atccctatat tgatgccgaa
    15481 gttatagaag tttcttggag atttctaaca agtgtaggca tcgatgatat ttctctaagg
    15541 atcaacagta ttggcgactc gaactgcaga ccaaaatacg ttgatgcttt gcaaacctac
    15601 tatcaaaatt actcccaatc tctctgtccc gattgtgtaa ggcgattgga gaccaatcct
    15661 cttcgtcttt tggattgcaa aatggaatct tgccaaccag tgattcaatc tgctccgaaa
    15721 agtaccgaat atctttgccc tccgtgcaat gctcactggg tccagctcca agaatattta
    15781 gaaaccactg gtataggata cgaaattgac aacacccttg tccgaggcct ggattattat
    15841 tcaagaactg tttacgaaat aacccctcct acggaaagaa ggaccaatgt tatagccgga
    15901 ggaggccgct atgatggtct gctagaacaa ttgggaggcc caacaacacc tggcataggt
    15961 tttggaatgg gaatagagcg agtgatcgaa aacattagaa ggcttgaaat aaatatcgca
    16021 tctaatccca aactaagcat tatgtttgca cacatcggag aaggagctaa aaaagaagcc
    16081 attagcctat catctcaaat aagacgatta ggaaaagaag cttttgtcgg cccttcaagg
    16141 ggattaagaa gtcaaatgcg atatgcatct cacatgaatt catcccacgt aataatcatt
    16201 ggggatgatg aattatccac gggtttatac actctgagag atttgagcaa aggtgatcaa
    16261 accgaaatta ctgcggatgg aattttaaat tttgttaaaa cccatgacat tggccagaag
    16321 gtagattaaa tgcttcaaaa cgacccctca gtcttcgaaa tgatttcaca agctcaagaa
    16381 tacatgcaag aaaacgaaat tgatggctgg cttctctatg actacagggg aatgaatccc
    16441 attttatggg acgccattgg ttacatatca aatgtaacta gaccatgctg gctatggata
    16501 cctgcaaatt ctgatcctaa attcattgta agttttgtag atcaaaatag gtttgaacag
    16561 ataggaataa aagcggaatt ctgggtaagc cgggaacaaa tgattgactc cttgaaatct
    16621 cacctacctc aacacggaaa agttgctatg gaatactcac caatgggagt tttacctaga
    16681 gtcgcaaaaa ttgatggcgg caccttagag ctaattcgaa gtctgggggt tgaagttgtt
    16741 acatcagcta atttaattca atacgctaca cagagatggt ctgacaaaga gctggattcg
    16801 catttaaagg cctctgaaat tttgaccact acagttaaag aagcattcaa cttcattagt
    16861 gaaaacttag atactaacct aacggaattc gaggttgctg aatttatacg aaatcggttc
    16921 acaaaaagta atcttataac tcccgatggc ccagtcgtag ctataaacaa ccatgcaacg
    16981 gaccctcact ttgagcccac agaagctaat tcttcaacaa tgagtaaggg agattgggtc
    17041 ttaattgact tatggggaag aataggagat gaaccctctg tttgtgccga tataacctgg
    17101 acagcattca taggtgacga ggtaccgaaa aaacatctag aagtatttaa caccgtcata
    17161 ggcggtcgcg atgctgcagt tactctgcta gaaaaagcct atcatgaggg aagagagcta
    17221 cagggttggg aagttgatgc tgaagctaga aggtacatag ataacatggg atatggcaaa
    17281 tatttcagtc atagattagg acatagtcta ggcagagagg cacatggaaa tgccgtcaac
    17341 ctagatggat gggaaaccat cgacacccgc actataagcc caaaaatcgg atttaccata
    17401 gaacccggca tatacatacc tggggaattt ggagtcaggt ctgaaatcga tttgttctat
    17461 tatgaaactg ggcccacagt cactactgaa agacaaacgt cgccgtatct aattcaggta
    17521 tcatgaaatc atattacttg atttcagtga gcttgttgtt ggttaattgt cttaggcaat
    17581 ttcaatccag ccaaaattgc tgtggatatc aaggcgacaa ctccgctgaa aatgaaacaa
    17641 cttttgatac ctaaatcagt gtcagataaa agcccggcta tcactggtcc tacagtcccc
    17701 aaaaaacttg ccccataaat taacgagact acagtggatt gtgcccttcc ttttgcaacg
    17761 tccatggctg ctgcaataaa tatcgtgtgg agtgaatata caaaggcacc cattgccaac
    17821 acattgagaa cgagttggac accatcatca gcaacactaa gagtcaaata gagaactcct
    17881 aggagagaca ttgcaggcac tagcacaatt ttccttccgt atttatccga caaccaaccc
    17941 ataataggtt gcgcaacaat tccagttgcc tgtgcgcctg caatgaaaag tgctgttgtg
    18001 attgcatctt tcccaagtgt atcaatcaaa tacaccggca agaatgcaac gatcgtcgct
    18061 tgagccatgg accgaaaagc agtaacaaag ataaggccta gcatgatttt atctttgagc
    18121 aataagccaa catccccaaa ataatctctt agtgatgcag tatcactatc aacagttgga
    18181 atatttctca tcatcaaata aacaagtaca gcgaataata aggctgggaa aacactgatt
    18241 ttaagaactt cattccattg gaaagctact aagtaagtaa gcgatataat tgaggctgtt
    18301 tttacagact cagtcgttag caacccaacc acaataggtc caagcatttc cccaacagaa
    18361 ccacctgtac catgcaggga tatagcaaat cctcttcggt ctggaaattt cctcgacaat
    18421 gaggctattg ctggtgggtg atatagagat ggcccaatgc caaccaatag catcacagcc
    18481 aatacccacc aaaagctggg tgcaaacccg agaaggaaat acgatatacc catcatgctc
    18541 agagagataa aaagcattag tccggctctg tttgcaaacc tctcccctaa gtaccctgca
    18601 accatagttg tcgcgccact cgtccctctg ccaacactag taataacgcc gtattgtgtg
    18661 tttgataaac ctaagtcacc tttcattaat gtcaaaagaa taggcaaagc tgaattgaat
    18721 acatgcttga cggcgtgtcc caatgtaacg gttacggcta attggtttct tcctttccgc
    18781 aattcggggc ttacttcaac agactccatt taactctcca agaactcagt ggccaaaacc
    18841 ggtgaatcat aacatctcgt gatggacatt caatgaattc ccgattcttc agatttccaa
    18901 tgttcataga aatgattaag aggtccgtgg ccattcccga tctgtaaatc atgcctaatt
    18961 gagccggtta caaaagcttt tgcttcacta acagaatttc ttgtcgataa ccctttagca
    19021 ataccagcag cgattgcgga tgcgaaagta caaccagtac catgcgtgtt gagtgtgttg
    19081 actctcttag tcgtgaacag atgaaaggat gtcccatcat aaaatatgtc ggtagctggt
    19141 ccttcattta tatgaccacc tttgaccacg gctgttttac aacccatttc taccagtttg
    19201 acagcagcag ctttcgcact attcacatct acaacattta tacctgtaat ggcagtagct
    19261 tccggcccat ttggagtaac gactaacgca attggaatga gttctgtttt taaagcttct
    19321 atagcttcat cctgtaataa cgtatcacca ccttttgcga ccataacagg atcaacaact
    19381 acattctcga tttgatgttg ggataacttc cctaccacag ctctgattat ttcagaacta
    19441 gacaacatac cagttttaac agcatcagtt ccgatatctg tcaatacaga atcgatttga
    19501 ttttcaatta aagtgactgg caattccaat atatcttgta ccccagtagt attttgagcg
    19561 gttatggcgg ttaatgctga agttccatat actccatttg cggcaaaagt tttaagatct
    19621 gcttgaatac cggcgcctgc gccagaatct gatccagcta tcgtcaaaca ctttggggtg
    19681 gtcttcatac gtgccgtcat tgttgatccc ctaaattcat cttcgtttaa acatcaaacc
    19741 agagttcatc attgtacgca gattcccaaa acaaaaattc atatctaaga gctgtgtgaa
    19801 aaagataatc ggccctttca gccgatattg atgaatcatt atttatataa ttttctaacc
    19861 aagacaccaa atcccaaagt acttcaggcc catgtaggtc tatccattct ctgtaaacag
    19921 ggttagtcag actcgctttt tcttccctca accgagtacc ccaatctaaa taggtacctt
    19981 cggtgacata taacaccagg acaatttcat caaaagtacc ttccagagat gtcctaacta
    20041 aaaagtcacc aaaggcttga gtagtgggtg aagcctcaac agaagaataa tcttcttcag
    20101 atgctcccag ctgattaaaa aatcgcataa ataggtcgtt ttctgcgtct aacacgccag
    20161 tcagaaaatc attaaaaatc gatgcggctt tcaaatttgg agcttttgca agtgcttgag
    20221 ctgccataac gactaaatcg tttacaaaaa cataatcttg aagaaaatat gctttgaata
    20281 tactggggga caaggaccca tctcccaatt gggaaacgaa ccgatgataa gtcaccaatt
    20341 cccacagccc gcaatatttt gcttttagat cagaagttaa tgtcactgtc cagaaccttt
    20401 attactttat ttagcagaaa attcatctgt gaaagcagct tttggatcta gattttcatt
    20461 tataatgcct ctcactttca tccaattaga aaaagatacc catctggcct cttcttgagt
    20521 cccaaaccta ccgttctttc ccttccacaa atcctggagc agatctgccc caggcctatc
    20581 gatatcttca tctatttcag gggaatattt ttttaagata tcaacaccgg attgcggatc
    20641 agaaattgca tcagcgtatc cctttccaac agcacgggtg aatttttcga ccacatcgga
    20701 attttctgac aaatagtttt cactcgttac catcaccaat tcgtaatagt cggggacacc
    20761 ccattcttcc atccgcatca cattgacagg atgcccttca ttttcaagaa gtatgctctc
    20821 atgagtaaaa taagctccaa taatcgcatc tactgtttca gatatcattg acgaccccaa
    20881 ttcccatcct acattcacta gctctacatc atctagcccg cttataccat cagactcaag
    20941 catggtattc aaggcatctt cattccaagg aatacctgga taccctactt ttttcccttt
    21001 cagatctttc ggggttttgt ttccggaaga ctgtaatgcc atcactgagt tcaacgggtg
    21061 ctgcaccata cccaaaattg aaattatcgg aactccttct gaacgggcta tcagaacatc
    21121 aggttggtaa ttcataccaa aatcatctgc acctgaagct accgtttgta aaactgtaga
    21181 aggatccgat ggggtatata tttcaacttc taggttttcg tcttcaaaat aacctttttc
    21241 ctgagcaatg tataacccaa tgtgattagc atttggatac caatccaacg caagtttgac
    21301 ttgaacaatt tctgttgagg gacggcagga aaaattcaag caagctaaca ctgtcagcaa
    21361 gatggatcct attaatttat ttttcatcta tcatccttta ggggttttat tccgtaaatt
    21421 taatttctct tttcatgggc ccacggcaca aacaccctct ccaaataggt tattaaagca
    21481 aataatccta cccctataaa agatagaatt atgatcgctg caaatactcg ctcagtttga
    21541 aattgtggct ttgatcgaat catcagatac ccaagtcctt cgctagaacc aacccactca
    21601 ccgattaccg cacctatcac gctgatcgct atcgcgaccc taagacccga aaataaataa
    21661 ggaagcgagg atgggaccat aattttcgaa aatatttgag cttttcttcc ccctaatgta
    21721 tgcatcaact tttggatatc aggatcgatg gatttcatgc catctacagt attaaccaca
    21781 atagggaaga aactgatcaa agctaccact attattttag gtgccaatcc atacccaatc
    21841 caaaccaata acataggagc tataacaata attggtacag tttgtgaagc gattataaac
    21901 ggatataaag ctttttcaat tgtctgcgaa agacaaatag cggaggctaa aatgaccccc
    21961 acgcacaagg ctaaaaaaaa tcccaaaata atctcttcta gggttacgat agtatgccgc
    22021 ataagcatac tgtgatcatt gtatatagtt gttgccacag aagatggtga gggtaacagc
    22081 caactaggta tttcttgtat tttcacaagt aattgccatg ccccaacaat taaacatact
    22141 attccaaaag cggggctcca tttgaccaac caatttatca tcatttaatc ctgatcccat
    22201 agaaaggaaa ggattttaga tttcaatttg acaaatgaac tgtcattgac gatctcacgg
    22261 tttctaggtc ttgaatatgg cactttttct actgaattaa tccggccagg ccgggatgtc
    22321 atcacataaa tcctgtccga aagcataata gcttcatcca catcatgagt gaccaataaa
    22381 atagtttgct gtatcccatc cagcaaatcc agcaaccaca actgcaggcg agacctattt
    22441 aatgcatcga gggcactaaa tggttcatca aggagaagaa tatctggttc agtcattaca
    22501 gtcctgagaa atgaagctct ttgtttcata ccccctgaca aagtatgcgg gtagtttgat
    22561 tcaaagccag ataacccaaa agattgaaaa tatggctcaa ccatttgata agacttcttc
    22621 tttgatacgc ctttgatttc caaaccaagt atgacattgt cttttactga tcgccatggc
    22681 agtaaaaggt ctttttgctg catatatccc actgacccca atctagaatc aaaatccaac
    22741 ccatctatat tgatgtcccc ttcatcaggc tcgtcaagac cagcaataat gttaaggagg
    22801 gtactttttc cgcaaccact cggacctaat acagatataa tttcagattt ctgaatcgat
    22861 aaatccaccc cgtccaaaat tttcaacggg cctccgtcct gacaaatggt cttctcaaga
    22921 ccagcaatgc ttatgtgatt gtctactatt ttttccattc aagtctagtc tctgatgaaa
    22981 aaatccaaaa aaaaaccgcc cactatgtgg cggccaggat attttgtgct ttttaaattg
    23041 ggatccctcc gctggcatta cccagatcag gtatttaatg ggtcgacaac atcagcgttg
    23101 ccctctcagc ctggccgttc caagctcccc caatttggtt taaattctta atggtcggat
    23161 tctagcataa aattaccaca aaatatgtga ttactgggaa gtttccaata tatcgcgagg
    23221 ccgaggagaa ggaggcagtg ccataagaac caataacatg gacacaattg aggctgctgc
    23281 gaatcccaaa aaggcccatt cgtaactacc cgttctatcg tatacaagcc cagatactac
    23341 tggaccgaca ataatcccta acatttgaat gctgcttctg aagccagaaa ttgtggcgaa
    23401 tgccttcctc ccaaaatagt cggcaataat ggactgcggt atgacagaaa ttcccccttg
    23461 agagattgaa taggcaacca atccgggaat ggatgaagac agggagtcgg ctgtagctag
    23521 taa
//
LOCUS       DASCNS010000202         1458 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001555325, whole genome shotgun sequence.
ACCESSION   DASCNS010000202 DASCNS010000000
VERSION     DASCNS010000202.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001555325"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="QF838_07365"
     CDS             <1..241
                     /locus_tag="QF838_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60534.1"
                     /translation="PIERFMSGHGPIGDHNALVEAKDFIHTLVGSVKHAIADGQDSLQ
                     ASKYVINEMSPKYGDWRSFDRIGENLPSVYDKLKV"
     gene            331..>1458
                     /locus_tag="QF838_07370"
     CDS             331..>1458
                     /locus_tag="QF838_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60535.1"
                     /translation="MCNIMGFLIANTIGILLPSISADLNLTPTQQGILSSAPFWANVG
                     LMIVIALWVSRYAPKILTLVTVILGGALILLQAWAQGFFVIFVGRFLFGITMIAREPA
                     RSLLIRQWLPQREVNHAGGISNLFFGIMVSGGVFLLPMLLNYFNGNWRTVMVTFAFIF
                     FGLAIIWAIIGKENPLAEPEEDEKSNEFQIILRIFRFKDIWSAGIGFLGVLMSFASFN
                     SFLPTLFVDSYDLSLGKSGLIVALYILPGGFSGVLVGLFCKSPKSRSLILMLAGLVIT
                     ITFAAMTLVENYNWLILISFTNGLAWGFFPLLYMVPFHISGIKPREIAVSVATVMTMA
                     SIGGSIGPTLTGYLQESLGSLEISLRYVSIAPMTLLVAGILL"
BASE COUNT          373 a          299 c          316 g          470 t
ORIGIN      
        1 tcctatagaa agatttatgt caggtcacgg cccgattggt gatcataatg cccttgtgga
       61 ggccaaggat tttatccata cccttgtggg ttccgtcaag cacgcaattg cggacgggca
      121 agattcatta caagcaagta agtacgtaat aaatgaaatg agcccaaagt atggtgactg
      181 gagaagcttc gatcgcatcg gtgaaaacct tccgagcgtt tatgacaaac taaaagtcta
      241 gtctccatgc ccctccaatc gagcattaaa gttctaggta aaaatacgac acctagccct
      301 tatagatggg tggtttacac actctttgtt atgtgtaaca tcatgggttt tcttattgca
      361 aataccatag gtatattatt accttcaata agcgctgact tgaatctaac tcccacacag
      421 caaggcattc ttagttccgc tcctttttgg gcaaacgttg gtttgatgat tgtcatcgcc
      481 ctctgggttt ctcgatacgc tcccaaaata ctcaccttag tgacagtgat tttaggtgga
      541 gcgcttattc ttcttcaagc ttgggcccaa ggattttttg taatttttgt tggaaggttt
      601 ctgttcggaa ttactatgat agctcgagaa cctgcaagat cactattaat tcgacagtgg
      661 cttccccaaa gggaagtaaa tcacgcagga ggaatttcca atttattctt tgggatcatg
      721 gtcagcggtg gtgttttcct actaccaatg ctattaaatt actttaatgg caattggaga
      781 actgtcatgg tcacctttgc ctttatcttt tttggacttg caataatatg ggcgataatt
      841 ggtaaggaaa acccactcgc tgaaccggaa gaggacgaga agtctaatga attccaaata
      901 atcttaagaa ttttccgttt taaagatatc tggtccgcag gtataggttt tcttggtgtc
      961 cttatgtctt ttgcttcatt taatagcttt ttacccacct tgtttgtgga ttcctatgat
     1021 ttatccttgg ggaaatctgg attgatagtt gccttataca tcctccctgg aggattttcc
     1081 ggtgtgctag ttggtctatt ttgcaaatcg cctaaatctc gaagtctcat cttaatgcta
     1141 gccggtctgg ttattacaat aacttttgct gcaatgaccc tggtcgagaa ttacaattgg
     1201 ctgatcctga tatcttttac aaacgggttg gcatgggggt ttttccctct cttgtatatg
     1261 gttccattcc atataagcgg cataaaacca agggaaatcg cagtttccgt ggctacggta
     1321 atgaccatgg cttctattgg gggttcaatt ggcccgacac taactgggta tttacaagaa
     1381 tccctgggaa gtttggaaat atccctcagg tacgtctcaa ttgcgcccat gacgctattg
     1441 gtggcaggaa ttttactg
//
LOCUS       DASCNS010000203        11636 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001570037, whole genome shotgun sequence.
ACCESSION   DASCNS010000203 DASCNS010000000
VERSION     DASCNS010000203.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11636)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001570037"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="QF838_07375"
     CDS             <1..954
                     /locus_tag="QF838_07375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HJO60536.1"
                     /translation="RALFVGGSPDISFTLFIYDMRSQGKLQMVQESVENTIEASHFSK
                     PQPVKYVTTNAEECHAFYYPPNNANYEAPQATKPPLVILSHGGPTGATSNTLNLAIQY
                     WTSRGFAILDVNYRGSTGYGTRYRKALVGNWGINDVDDCVNGGNYLVSENLVHPEKIA
                     IRGGSAGGFTTLACLTFTDFFAAGASYYGVSDLTTLAEETHKFESRYLDSMIGKYPEE
                     RQKYLDRSPINHTENLSCPVILFQGLDDEIVLPNQSQKMYASLKAKGVPVSYLEFEGE
                     QHGFRKSENIQRALEAEFYFYSHVFGFKPFDPIEPVEIDNK"
     gene            complement(958..2052)
                     /gene="dinB"
                     /locus_tag="QF838_07380"
     CDS             complement(958..2052)
                     /gene="dinB"
                     /locus_tag="QF838_07380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HJO60537.1"
                     /translation="MSNRNHNRGILHADFDAFYAAVEQRDFPELRDRPVVVGGSPSKR
                     GVVASASYESRKFGIKSAMPTSIALRKCSEAVVVSPRFAVYREVSRQVMEVFRDITPL
                     VEPLSLDEAYLDVSHLIAGTITPPQIAKKLKQKVLSKLGLTISVGVASSKSVAKIASS
                     INKPDGLTIVRPGGEIPFLYPLPVEVLWGIGPKTSAKLHLLSIYTVEDLASQPMKILS
                     GIFGNNATHIKEISNGIDTTPVITQRERKSISSETTLLVDTSDAEDLSEIVMRLSGYV
                     AESLLKKNMRGRTIKLKLKRSDFTVVTRQKTIPEKVQISSEIAESALELLNFELENSD
                     EKYRLIGVGVSGFATSENLQDEMQLRFEGL"
     gene            2320..6168
                     /locus_tag="QF838_07385"
     CDS             2320..6168
                     /locus_tag="QF838_07385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HJO60538.1"
                     /translation="MTSSALQDNSVVFPKTKSFSKIKTTLDVPDLIDVQMESFKWFTT
                     TGLSELFTEISPIEDNQGQNPRFSLRFVDFEFEEPKLSEEYCRSQEKTFEAALYVTVS
                     LQINAAGPGMGEIKEQRLYVGNIPIMTTGGTFIINGAERIVVSQLVRSPGVYFTADRD
                     AATGRPLASAKMIPYRGAWIEFETSNRDVIYVKIDRKRKTPVTTFLRSLGYETNEEIL
                     EIFKDVDNNPDHPFLQTTIEKDASVTNQEEALVEFYRRLRPGEPPNSENAKALINSLF
                     FDSRRYDLGKVGRYKLDRNLKGAENADKDGNLEDRILAKEDIVNLLKRLIQVNNSERR
                     ANDIDHLGNRRVRAVGELIQNQVRVGLLRMERVIKERMTIQPDPESTTPAALINVRPV
                     VAAIREFFGGSQLSQFMDQANPLAELTHKRRLSALGPGGLSRERAGFDVRDVHHSHYG
                     RICPIETPEGPNIGLLVSLATYARTNEFGFIETPYRRILTEVDNGDPDELIGHTPTED
                     IKAEDGRTIVKAGTRISVRTAGRISAQKNKIIEVRPYVSNDIADIVHLAADDEEDYYI
                     AQANSAINEAGEFLNDRVETRRGEEVGMEQTDSVRFMDVSPLQLVSVSTSLIPFLEHD
                     DANRALMGSNMQRQAVPLLKPEKPLVGTGMEMRVARDSGQVLMSKAEGEITSVTGNEI
                     VVTDANGKKFEHSLIKFHRSNQGTCINQRPIVDKGTTVRIGDVLADGSSTDNGELALG
                     QNVLVAFMTWEGYNFEDAIILSNRLIKEDKFTSVHIEKHEIEARDTKLGPEEITRDIP
                     NIGEDSLRNLDEEGIIRAGAEVSNRDILVGKITPKGETELSAEEKLLRAIFGEKARDV
                     KDSSLYVPHGQGGKVIDVKILDRNNGDDLSPGVNKLVRVWIAHTRKITEGDKMAGRHG
                     NKGVIAKILPEEDMPFLPDGTPVDIILNPIGVPSRMNLGQVLETHLGWAAKRLGFDAK
                     TPVFDGAEDSAIEDELAKAWIIEQSGAVPARAIVDNASEIIDLGMISEFLEKRGFDFE
                     EIFITSKPNSARYACLKIWMDEVANKNTTDMSVDDLMEEALVLNRDHSLAAPVLGKST
                     LYDGRTGDVFDQPVTVGYIYMLKLIHLVEDKIHARATGPYSLITQQPLGGKAQLGGQR
                     FGEMEVWALEAYSAAHNLREMLTIKSDDEIGRVKTYEAIVKGEDVAQPGIPQSFHVLM
                     KELQSLVLSIELENDDVIEEQGFGGSDDLFSDSSAISDPLALLEGSLNSNGSEPEGDS
                     ETNEEPESIEGDQPVPGGDD"
     gene            6213..10271
                     /gene="rpoC"
                     /locus_tag="QF838_07390"
     CDS             6213..10271
                     /gene="rpoC"
                     /locus_tag="QF838_07390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HJO60539.1"
                     /translation="MAQPGNDFDAISISLASPNDVQRWSWGEVTKPETINYRTLRPEK
                     DGLFCERIFGPTKDWECYCGKYKKIRFRGVTCDRCGVEVARSKVRRERMGHIDLAAPV
                     AHIWFSKGTPSRLGLLLDLSPRNLDRVLYFAQYLITDLDVELKGQLLEQLHSNLEGEN
                     LEKESKIESAENTLTEEYQSQIEPLQGLLHEENSDNGDEDKKSSEEQLGTEGQIESLQ
                     KERDQKIEELIAEINDEYEELLSAIENQISSLEELHVTKLLTETQYRDNRDNFPGVFQ
                     AGMGAESVLYVLENHIDLDDLRDELQEEMQSTSGQRRKKAIKRLRVVESFRNSTNKPE
                     WMILTNLPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLSRLIELQAPEIIIR
                     NEKRMLQESVDALIDNGRRGRAVAGSHNHKLKSLSDLLRGKQGRFRQNLLGKRVDYSG
                     RSVIIAGPELKLHQCGLPRRMALELFKPFVMHRLVLKGYAHNIRSAKRLAERNRSEVW
                     EILGEVVKDRPVLLNRAPTLHRLGIQAFEPVLIEGSAIRVHPLVCTAFNADFDGDQMA
                     VHVPLSRIAVLEARKLMLSTFNMLAPSSGEPIVTPSLDMVLGCYYMTSIDENAHGIGK
                     KFSNFEDAILAHEVGAISLRAPIFARNNSGELIDTTVGRIIFNSVLPDNLPFVNEEVE
                     RNKLKEITGNCFRLLGNEETAVVLDDIKNLGFRNSSKSGVSIAINDVKVSPKKSEIVS
                     EAEEAVTQLEEQYMDGLITEDERYNATVRIWTDANDKLTQVIAEDLPNYGGIYMMANS
                     GAKGNIAQIRQMAGMRGLMSNPRGRIIDRPIKSNFREGLTVLEYFISTHGARKGLADT
                     ALRTADSGYLTRRLIDVAQEVIITVEDCGVGRGITVEDYHDDTLKDLFYDRIKYRYTA
                     SPVANPKTGEIIIEADQALQEEDIEILKELEVTVVEVRSPLSCQAEKGLCKMCYGLSL
                     ANLRPIMIGDAVGIIAAQSIGEPGTQLTMRTFHTGGVAGADITSGLPRVEELFEARSP
                     RGAAVLSEISGKAEILETPEGRTARIINAEELVDEYALEGYKPLVKKGSTVIIGTPIA
                     ASKDSEDDSVIHARNSGTVGIEGATITITWTDEEQREYAIPAASHLEITNGDEVEAGE
                     AITSGPKNPQQILEIQGRESVQSYLIEEVQKVYRSQGVPIHDKHIETIIRQMLRRVQI
                     NEAGDTEFLPGDPMDRKGFEGKNGEIIAEGGDPATAVPILLGITRASLHMDSFLAAAS
                     FQETTRVLTESAVTGKRDVLSGLKENVIIGRLIPARYDITEEGREKLGVAELEKMFDM
                     NQLPESPPPPALEDGSEMSILTAEINTLEEPQ"
     regulatory      10345..10474
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10593..>11636
                     /locus_tag="QF838_07395"
     CDS             10593..>11636
                     /locus_tag="QF838_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013648.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60540.1"
                     /translation="MFRRVLLSTLIAFVLTIGMASCSAGGSPKKPDPAAPASIPVPIK
                     TQPKKQILPSTPVTPPTPLPTTKEIKIQSPPKGRVVQYFPPPSPPTDKKLTAKFPLTV
                     ISRDGIEMIFNKPPDRIIAYDAAAVETIFAIGQGHRIIGTHSWVSHPVEVGNIEKVGD
                     AFNMNIERILDLNPDLVFLFYPTFKEELEEAGLKVLLLESVDTDLMKMADTFRTWGQI
                     TGAVNEAEILAQNYESRITEIKLVLDPYHSGPSIFQDVGDLWTPGNNTLMGSVFQLLK
                     LKNVAHDVDNYGQLSPEVIIERNPQIIISSTPDLMYRNPAFNELLPIKKREMVRIGRY
                     WTLRNPKYTFEGQS"
BASE COUNT         3750 a         2348 c         2648 g         2890 t
ORIGIN      
        1 agagcgcttt tcgttggtgg ttcaccagac atatcgttta ccttgtttat ttatgatatg
       61 aggtctcagg gaaagctcca gatggtgcaa gaatcagtag aaaacacgat tgaagccagt
      121 catttttcaa aaccacagcc agtcaaatat gtgacaacaa atgccgaaga atgccatgct
      181 ttttattacc caccaaataa tgcaaactat gaagcacctc aagccaccaa gccgccactt
      241 gtaatactca gccacggcgg ccctacagga gcaacttcaa ataccctcaa cttagccatt
      301 caatactgga caagtagggg gtttgcaatc ttggatgtca attacagggg aagtactgga
      361 tatggcacca gatacagaaa ggctcttgtc ggtaattggg gaataaatga tgtggatgat
      421 tgtgttaatg gtggcaatta tcttgtttca gaaaacttgg tccaccccga aaaaatcgct
      481 atacgaggtg gaagtgcagg aggatttaca acattggctt gtctgacatt cacagatttt
      541 tttgcagcag gagcaagtta ctatggtgtc agtgatctta ctaccttagc tgaagaaaca
      601 cataagtttg aatcaagata tcttgattct atgattggaa aataccctga agagaggcag
      661 aaatacctcg atcgttcccc cattaatcat acagaaaatt tgtcctgccc cgtaattctg
      721 ttccagggat tggacgatga aatcgtactt cctaatcaat ctcaaaaaat gtatgcgtca
      781 ttgaaggcaa aaggggtccc agtatcttat cttgaattcg aaggagagca gcacggattc
      841 aggaagtcag aaaatatcca gcgtgccttg gaagctgaat tttactttta ctcacacgta
      901 ttcggcttca aaccttttga tccaatagaa ccagttgaaa tcgataataa ataactttta
      961 cagaccttcg aacctcagtt gcatctcatc ttgaagattt tcagaggtag caaatcctga
     1021 tacaccaact ccgatcaaac ggtatttctc atctgaattt tctaactcga aattcaaaag
     1081 ctctagggca gattccgcta tttcagatga gatttgcacc ttttctggaa tcgttttctg
     1141 tctagtcaca acagtaaaat ccgatctttt gagttttaat tttatggtcc ggcctctcat
     1201 attctttttt agtagtgatt ccgctacata gccactcaat ctcataacta tctcactaag
     1261 atcttccgca tcagaggtat ctaccaataa agtggtctca gaactaattg atttacgttc
     1321 ccgttgcgtt atcacgggtg tagtatcaat cccattagat atttccttaa tatgagtagc
     1381 gttatttcca aaaataccag aaagtatttt cattggttga gaggctaaat cttccacagt
     1441 atagatcgat agcagatgta atttagccga agttttcgga ccaattcccc ataaaacctc
     1501 gacgggtaac ggataaagaa agggtatctc gcccccaggt cgtacgatgg tgagtccgtc
     1561 aggtttatta atgctagaag cgattttggc taccgatttg gaactcgcaa ccccaacaga
     1621 aatcgtcaga cctaattttg acaaaacttt ttgttttaat ttttttgcaa tttgtggagg
     1681 ggtaatagta ccagcaatta aatggctgac atccaagtat gcttcgtcca acgagagtgg
     1741 ttcgactaaa ggggtaatat ctctaaatac ttccattact tgtcttgata cctctcgata
     1801 cactgcaaat cttggtgaaa caaccacagc ttctgagcat tttcttaagg caatgcttgt
     1861 aggcatggca gatttaatcc caaattttct ggattcataa gaagcagatg cgacaacccc
     1921 tcttttactc ggactgcctc cgaccactac cggcctatct cgcaactcag gaaaatctcg
     1981 ctgttccaca gcagcataaa aagcatcgaa atctgcatgc aatatgcctc tgttgtgatt
     2041 ccgatttgac aatttcttcg atagacctca actgggctcg cattttaaaa ttattctagc
     2101 tatcgtctat actttggtcc atgcctcatt agtattgaca agcgtggtat gccgtagcta
     2161 aaataaaaga ctgtatgatt acacatgcgc tggtttgcac aaacgtttta acaaagccac
     2221 ctatcttggc gtttttaaat taccactctt ctatcttaga agagtgtttt ctcccgtaac
     2281 aactggaaat atttctttcc ctcaaggagt gtaagtctaa tgacctcttc tgcattgcag
     2341 gataacagtg ttgttttccc aaaaacaaaa tctttctcca aaattaagac gactttggac
     2401 gtccctgacc ttattgatgt tcaaatggaa tcattcaaat ggtttacaac gaccgggtta
     2461 agtgagctat ttacggaaat atcacccatc gaggataacc aaggacagaa tccacgtttt
     2521 tccctcagat tcgtagattt tgaatttgaa gagccaaagc tctctgaaga atattgccgg
     2581 agccaagaaa aaacattcga ggctgcttta tatgtaactg taagtctcca aatcaacgca
     2641 gccggaccag gtatgggcga aatcaaagag cagcgtctgt atgtaggaaa tattcccatc
     2701 atgacaacag gcggtacctt catcataaat ggagccgaaa gaatcgtagt gagccagctg
     2761 gtgcggtcac ctggggtgta ttttacggcg gatagggatg ccgcaactgg acgccctctt
     2821 gcatctgcaa aaatgatccc gtaccgagga gcatggatcg aatttgaaac aagtaataga
     2881 gacgtaattt acgtcaaaat tgataggaag agaaaaaccc ctgtcacaac cttcctcaga
     2941 tcattaggct atgaaaccaa tgaagaaatc ctggaaatat ttaaggatgt tgacaacaac
     3001 ccagatcatc catttttgca gaccaccatt gaaaaagacg cgagtgttac caaccaagag
     3061 gaagcccttg tggaatttta caggcgcttg agaccaggcg aaccacctaa ttcagaaaac
     3121 gcgaaagctc taattaattc ccttttcttt gacagtagac gttacgatct aggtaaagtt
     3181 ggtagataca aattagaccg gaatctaaaa ggagccgaaa acgcagataa agatggtaat
     3241 ttggaagatc gtattctagc aaaggaagac atcgtcaacc ttttaaagcg acttatacaa
     3301 gttaacaact cagaacgtcg agcaaatgac attgaccatt tgggtaatag aagggtccgg
     3361 gccgtaggtg aattaattca aaatcaggta cgcgtagggc tactgcggat ggagcgggta
     3421 atcaaagaaa ggatgacgat acaacctgat cccgagagca ctacccctgc agccttgatt
     3481 aatgttcgtc cagtagttgc agcgattaga gaattctttg ggggatctca attatcgcaa
     3541 ttcatggacc aagccaaccc tctagctgag ttgacccaca aaagacgttt gtccgcattg
     3601 ggccctggtg gattatcaag agaaagagcc gggtttgatg tcagagatgt tcatcactcc
     3661 cattatggaa ggatctgccc tattgaaacc ccagaaggac ctaacatcgg actcttagtt
     3721 tcattggcaa cttacgccag aaccaacgag tttggtttta ttgaaacacc ctatagaagg
     3781 atacttacag aggttgataa tggcgacccg gatgaattaa taggccatac tccgactgaa
     3841 gatataaagg ctgaggacgg tagaactatc gtcaaggctg gaacacgaat atctgttagg
     3901 acggcaggaa gaatttcggc acagaagaat aaaattatcg aggttaggcc atacgtttcc
     3961 aacgatatcg cggacatagt tcatttggct gccgacgacg aggaagatta ttacatcgcc
     4021 caggcgaatt cagccatcaa cgaggcaggg gaatttctta acgatagggt tgagactagg
     4081 aggggggaag aagttggcat ggaacaaacc gactccgtcc gattcatgga tgtgtctcca
     4141 ttacagttag taagtgtatc tacatccctg atcccgtttt tggaacatga cgatgctaac
     4201 cgtgctctta tgggttctaa tatgcaaaga caggctgtgc cgctattaaa accagaaaaa
     4261 cctcttgtag gaactggtat ggaaatgagg gtggcaaggg atagtggaca ggtgttgatg
     4321 tctaaggctg aaggggagat tactagtgtt actggaaacg agatagttgt aacagatgca
     4381 aatggtaaga aattcgaaca ctcacttata aaattccacc gaagtaacca aggtacctgt
     4441 atcaaccaga gaccgattgt cgataaagga acaacggtgc gaataggtga tgtccttgct
     4501 gatggatcat ccacagacaa cggagagttg gcattgggac aaaatgttct cgtagctttc
     4561 atgacttggg aaggttataa ctttgaagat gcgattattc tcagtaacag gttaataaaa
     4621 gaagataaat ttacatccgt acatatcgaa aaacatgaaa ttgaggcaag agataccaaa
     4681 ttaggcccag aagaaattac ccgagatatt ccgaacatag gtgaagatag ccttagaaat
     4741 ttggatgagg aagggataat tagagcaggt gccgaggtat caaatcggga catactggtt
     4801 ggcaagatca cacccaaagg agaaactgaa ctttctgcag aagaaaaatt acttagggct
     4861 attttcggag aaaaggcccg tgacgtaaaa gattcttcct tgtacgtacc tcacggacaa
     4921 ggaggaaagg tgatcgatgt caaaatacta gataggaaca acggtgacga tttatctcct
     4981 ggtgtcaaca aactcgtcag agtatggata gcccatacta ggaaaataac tgaaggcgac
     5041 aagatggccg gccgacacgg taacaaaggg gtaatcgcca agatcctacc tgaagaagac
     5101 atgccttttt tgccggacgg cacgcccgta gacataatac ttaaccctat cggggttccc
     5161 tcaagaatga acttggggca ggtactggaa acccatctgg gttgggcagc aaagagatta
     5221 gggttcgatg ccaagacccc agtatttgac ggtgcagaag attccgccat agaagatgaa
     5281 ttagcaaagg catggataat cgaacaatca ggagcagtcc ctgctagagc aattgtagat
     5341 aatgcttcgg agataattga cctcgggatg atatctgagt ttcttgagaa aagaggtttc
     5401 gattttgaag aaattttcat aacctccaag ccaaactctg ccagatacgc ctgtttgaaa
     5461 atatggatgg atgaagtagc gaataaaaat acgactgata tgtcggttga tgatctcatg
     5521 gaagaggcct tggtgcttaa cagagatcat agcctcgctg ctccagttct gggtaagtcc
     5581 acactatacg acggtaggac cggtgatgta tttgatcaac cagtgactgt cggttacata
     5641 tacatgttga aactaattca tctggtggaa gataaaattc acgccagagc cactgggcct
     5701 tattccctga taactcaaca gccactgggg ggtaaagcac aacttggtgg gcaaagattt
     5761 ggagaaatgg aggtatgggc cttagaagca tacagtgcag cccacaacct gcgagaaatg
     5821 ttgactatta aatcagatga cgagatagga agagttaaaa cgtatgaagc aatcgtgaaa
     5881 ggagaagacg ttgcccagcc aggtataccg caatccttcc acgtgctcat gaaagaattg
     5941 caaagtctag ttctatctat tgagttagaa aatgacgacg tgatagagga gcaagggttt
     6001 ggaggtagtg atgatctttt ctctgattct tctgcaatct ctgatccttt ggcgctactt
     6061 gaaggaagcc tcaacagcaa tggcagtgaa ccagagggag attcagaaac aaatgaagaa
     6121 ccagaatcga ttgaaggtga ccaacccgta cctggtgggg atgactaggt aaataattac
     6181 accgacttct taacggctat ggagaatatg aaatggctca acctggaaat gatttcgacg
     6241 caatatcaat ttcattggca tcccccaacg atgtacaacg atggtcttgg ggtgaagtaa
     6301 ctaaaccaga aacaatcaac tacaggaccc tacggcctga aaaagatggt ttgttctgtg
     6361 aaagaatatt cggcccaacc aaagactggg aatgttactg cggtaaatac aagaaaataa
     6421 gatttagagg cgtcacttgt gataggtgtg gtgtcgaggt ggctcgttcc aaggtcagaa
     6481 gagagagaat ggggcatatt gacctcgccg cacctgtggc tcatatctgg ttctcaaaag
     6541 gcactcccag caggttggga ttacttttag acttgtcccc tagaaattta gaccgcgtac
     6601 tttacttcgc ccaatatctg ataacggatc ttgatgtcga attaaaaggt cagctgttag
     6661 aacaactgca ctcaaactta gaaggggaaa atttagaaaa agaatccaag attgaaagtg
     6721 cggagaatac cctcacagaa gagtaccaat cacaaataga acctttacaa ggcctattac
     6781 atgaagaaaa ttctgataat ggggatgagg ataaaaaatc ttctgaggaa caattaggaa
     6841 ctgaaggtca gatagaaagt ttacaaaagg aaagagatca gaaaatcgag gaacttatag
     6901 ccgaaataaa cgatgaatat gaggagttat taagtgctat agaaaatcag atttcctcgc
     6961 tagaagaact acatgtaacg aaattgttga cagaaacaca atacagggac aatagagata
     7021 atttccctgg ggtgttccag gctggaatgg gagcggaatc tgtgttgtat gtgcttgaaa
     7081 accacatcga cctcgacgac cttagggatg aactgcaaga agaaatgcag tcgacttcag
     7141 gtcagagacg caaaaaggcg ataaaaaggc ttagggtcgt agaatcattc aggaacagta
     7201 cgaataaacc agaatggatg atactcacca atctgccagt cctgccgcca gatttacgtc
     7261 caatggtcca acttgacgga ggacgctttg ctacaagtga cctaaatgac ctatacagaa
     7321 gagttataaa ccgaaataac aggctcagta gacttattga attgcaagca cctgaaataa
     7381 ttatcagaaa tgaaaagcgc atgctacagg aatcagttga tgccttgatt gacaacggta
     7441 gaagaggtcg agctgtagct ggtagtcata atcataaact caaatccctt tcggatctgt
     7501 tgcgagggaa acaaggtcga ttccgacaaa atctgcttgg aaagcgagtt gactatagtg
     7561 gaaggtcagt gattatcgct ggcccagaat taaaactcca tcaatgcgga ttgccaagac
     7621 gcatggcctt agaactattt aaaccttttg taatgcatcg actagtacta aagggctacg
     7681 ctcacaacat aagaagtgcc aagaggttgg cggaacgtaa cagatcagag gtctgggaaa
     7741 tattggggga agtggtaaaa gatcgcccag tattgctaaa ccgagccccc acccttcatc
     7801 ggcttgggat acaagcattt gagccagtgt tgattgaagg tagtgcaata agagttcacc
     7861 cattagtatg cacagctttc aatgcagact ttgacggaga tcagatggca gtacatgtgc
     7921 ctctatcgag gatcgctgtg ctggaagcca gaaaattaat gttaagcacg tttaacatgc
     7981 ttgctccaag ctctggagaa cctattgtta ccccatcgtt agatatggtt ctggggtgct
     8041 actacatgac atctatcgat gaaaatgcgc atgggatagg aaagaagttt agtaattttg
     8101 aagatgctat tttggctcac gaagtcggtg ctatttcact tagggcccca atatttgcta
     8161 gaaataattc cggagaatta atagacacca cagttggcag gataatattc aacagtgtgc
     8221 tcccagataa cctaccattc gtcaacgaag aagttgaaag aaataagcta aaagagataa
     8281 ccggcaactg cttccgactt ctaggtaatg aagaaactgc ggtggtactg gacgacatta
     8341 agaatctggg attccgaaat tcctcaaaat caggggtttc tatagctata aatgatgtca
     8401 aagtttcgcc aaagaagtca gaaatagtga gtgaagcaga agaagcagta actcagcttg
     8461 aagagcagta tatggatggg ctgataacag aagatgagcg ttacaacgcc acagtcagaa
     8521 tatggaccga tgctaacgat aaattgaccc aggtcatagc tgaggatttg cctaactacg
     8581 gtggaattta catgatggcc aactctggtg caaagggaaa catagctcaa atccggcaaa
     8641 tggcaggaat gagaggcttg atgtcaaatc cacgagggag aatcatagat agacctataa
     8701 aatctaactt tcgagagggt ttaaccgtac ttgaatattt tatatccacc catggcgctc
     8761 gaaagggact tgcagatacg gccttaagga cagcagatag cggttacctt actagaagac
     8821 tcattgacgt agcccaagaa gtcataataa ccgtggagga ctgcggagtt ggcagaggca
     8881 ttacggtaga ggattatcat gatgatactc tcaaggatct attttatgac agaataaaat
     8941 acagatacac cgcatctcca gtggccaatc ctaaaaccgg tgaaataata atagaagccg
     9001 accaagcgtt acaggaagaa gacatagaga tactaaaaga gctagaggta acggtggtag
     9061 aagtacgatc tcctctatct tgccaagctg aaaaaggcct ttgcaaaatg tgctatggcc
     9121 tgtctttggc aaatttgcga ccaataatga ttggagatgc tgttgggata atagctgcac
     9181 aaagtattgg agaaccaggt acccagttga caatgagaac ttttcacact gggggagtgg
     9241 ctggagcaga tataactagc ggattaccta gagtagaaga attgtttgag gcgaggtcac
     9301 cgcgtggagc agctgtttta tcagagattt cagggaaagc ggagatactc gaaacgccag
     9361 aaggtcgaac tgcacgaata atcaatgcag aagaattggt ggatgagtac gctctagagg
     9421 gatataaacc tctagtcaaa aagggcagta cggtaataat cggaacccca atagcagctt
     9481 caaaagattc agaagatgat tcagtaatac atgcccgtaa ttcaggaact gttggaatag
     9541 aaggtgcgac gataacaatt acatggacag acgaggaaca gagggaatat gccatacctg
     9601 ctgcttctca cctagagata actaacggtg acgaagtgga agcgggagaa gctattacct
     9661 cgggaccaaa aaacccgcaa caaattttag aaatacaagg tagagaatcg gtccaatcct
     9721 acttgatcga agaagtacaa aaagtctacc gatctcaagg cgttcccata catgataagc
     9781 acatagaaac tataattcga caaatgctaa gacgcgtcca gatcaacgag gcaggagaca
     9841 cggaattcct gcctggtgat ccgatggaca gaaaaggatt cgaaggtaag aatggtgaaa
     9901 taatcgctga aggaggagat cctgccaccg ctgtacctat acttttgggg ataactcgag
     9961 cttcgcttca tatggacagc ttcctagctg ccgcatcatt tcaagaaaca acgagagttt
    10021 taacggaatc agctgtgact ggcaaaagag atgttctgtc aggcttaaaa gaaaatgtca
    10081 tcataggaag attaataccg gctcggtatg atataaccga agaaggtcgc gaaaaactag
    10141 gggtcgcaga actggaaaaa atgtttgata tgaatcaact tccggaatca ccacccccac
    10201 ctgccttaga agacggatca gaaatgtcca tactaaccgc tgagattaat accttagagg
    10261 agccccagta aaccacagaa gactaatatg ttcatactca attattgaca aattaattgc
    10321 ggtcatggtt tgatggagct gttaaaaaca gaatatgaaa atggtgcaag tgccctacag
    10381 ggtttaaaag aggaagtcgg tcgaaatccg acactgtccc gcaacggtag gctttaagaa
    10441 gcaagtccgg tcaaccacta agtgccacta tctaggtctt cgcggaaagg ccaggctgag
    10501 atgacaaccg gcccacacag aaaagagtgg gttttttatt ttcccgctga aggccaaaac
    10561 tagacaggtc tttgaataaa aagggaaata atttgtttcg gcgagttttg cttagtactt
    10621 tgattgcttt tgtattaaca atagggatgg cttcatgttc tgcggggggg tcaccaaaaa
    10681 aacccgatcc ggcggcccca gcctccattc cagtgccaat aaaaacccaa cctaagaaac
    10741 aaatattgcc atctacgccg gtaacacccc ctacacctct accaaccact aaagaaataa
    10801 aaattcaaag cccccccaaa ggtagggtcg tccaatattt cccgcctcca tcacccccca
    10861 ccgataaaaa attaaccgcc aaattcccat taactgtaat atctagagat gggattgaga
    10921 tgattttcaa taagcctccg gaccgtatca tcgcttacga tgcggcagca gttgaaacta
    10981 ttttcgcgat aggtcagggc cacagaatta taggaaccca cagctgggtt tcccatcctg
    11041 tggaagtagg gaatattgaa aaagtcggcg atgcattcaa tatgaatatc gaacgtattc
    11101 ttgatctaaa cccagattta gttttcctat tttatccaac tttcaaagaa gaattggaag
    11161 aagctggcct aaaagtactt ttattagaat cggttgacac cgacttgatg aaaatggctg
    11221 acaccttccg gacatggggt cagatcactg gagccgtgaa tgaagcagaa atattagccc
    11281 agaactacga gtcaagaatc accgaaatca aattagtttt ggacccgtac cattctggac
    11341 cctccatctt tcaagatgta ggagatctct ggactcccgg caacaacaca cttatgggaa
    11401 gtgtatttca attgttaaaa ctcaaaaatg ttgctcatga cgtagataac tatgggcaat
    11461 taagtcctga ggtaatcata gaacgaaatc ctcaaatcat aataagctct actcctgatt
    11521 taatgtatcg aaatcccgcc tttaatgaat tgttacccat caaaaaaaga gagatggtga
    11581 ggatcggaag gtactggact ctgagaaatc caaaatatac ctttgaaggt caatca
//
LOCUS       DASCNS010000204        12910 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001572913, whole genome shotgun sequence.
ACCESSION   DASCNS010000204 DASCNS010000000
VERSION     DASCNS010000204.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001572913"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(149..1207)
                     /gene="recA"
                     /locus_tag="QF838_07400"
     CDS             complement(149..1207)
                     /gene="recA"
                     /locus_tag="QF838_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HJO60541.1"
                     /translation="MDKKEKTTALELAISQIDKHFGKGAVMKLGDAQDVAVDSISTGS
                     PSLDAGLGMGGIPKGRVTEVYGAESSGKSTLTYHVMASAQRDGGTAAYIDAEHALDPS
                     YAARCGVDTENLLVSQPDTAEQALEICEYLVRSSAVDVVVIDSVAAMVPKAELEGDMG
                     DSHIGLQARLMSQGLRKLTSSISKSNTAVIFINQIREKVGVFWGSPETTPGGRALKFY
                     SSVRIDLRKIESLKQGTDIVGNRVRARVVKNKLAAPFKTAEFDIMFNEGISREGDLLD
                     LAVDHSIVKKSGAFYSFGEVQIGRGRESAKTFLKENPVLTDEINEMVKAALNPEPMDD
                     VIVPMDDFGSDEEVNKAI"
     gene            complement(1348..2223)
                     /gene="sucD"
                     /locus_tag="QF838_07405"
     CDS             complement(1348..2223)
                     /gene="sucD"
                     /locus_tag="QF838_07405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HJO60542.1"
                     /translation="MSILVDKSTKLLVQGITGKEGSYHAQRSAEYGANLVAGVTPGKG
                     GQNFNTTVPVYDTVQQAVKETSANASLIFVPPAFAADAIVESIDAGIEVIACITEGIP
                     VQDTIKVVRYLRDKEVTLIGPNCPGIISPGENFKMGIMPGHIHLPGRTGVVSRSGTLT
                     YEAVGQLTQLGIGQSTCVGIGGDPVSGVTFVDILEKFKDDPDTDNVVMIGEIGGTKEQ
                     EAAEYIKSEFKKPIAALIVGQNAPPGKRMGHAGAIITGSAGKAEEKQAALSAAGVSII
                     PGPGEIGDTLKELIS"
     gene            complement(2278..3435)
                     /gene="sucC"
                     /locus_tag="QF838_07410"
     CDS             complement(2278..3435)
                     /gene="sucC"
                     /locus_tag="QF838_07410"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HJO60543.1"
                     /translation="MKLHEYQAKEILAKYGVPIPKSRLARTGSQAATAAFEMGGTVVV
                     KAQVHAGGRGKGGGVKIVESPGAAEQAAEKMIGTNLVTFQTGPEGVPVNSVLIEEGIE
                     IENELYLGMVIDGASKGVVVIASEAGGMNIEEVAETSPEKILQIPIDPVLGLQPFQGR
                     KIAYGLNLEQEHVRPVASLVQNLYQAFIETDASLLEINPLVITKDGEVLAADAKLDID
                     DDAIFRHKDMQEMRDVSQEDPLEVEARSSDINYVKLEGTVGCIVNGAGLAMATMDVTS
                     SAGASPSNFLDIGGGADEEKVAKALGLVLSDDSVKAVLVNLFGGILRCDIAARGFLLA
                     AEQQPNRVRPMVVRMLGTNSDEGREILSQSSLNVILVDTLGQAAAEIKKLA"
     gene            complement(3497..3583)
                     /locus_tag="QF838_07415"
     tRNA            complement(3497..3583)
                     /locus_tag="QF838_07415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3547..3549),aa:Ser,seq:tga)
     gene            complement(3684..4457)
                     /locus_tag="QF838_07420"
     CDS             complement(3684..4457)
                     /locus_tag="QF838_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60544.1"
                     /translation="MQAQYPIDPRKNFPLPEAEILNLGPTEALAPLTDGEVIGGHTMP
                     WGSNYTFLLWVTAGENQCVRAIYKPKEGEKPLRDFSAGTLYKREQAAYEVSKLLGWPN
                     IPLTIIRDGPYGVGSMQLYLDCDPRITYFDMRDDSPDTLFPLAVFDLLVNNADRKAGH
                     CLLDGFKNIWSIDHGLTFHSAFKMRTVMMEYCGQQIPPTLIADMKKLRTYLNKDDKSL
                     GLLISTEEITALITRLDQIIENPIMPILDPNVSIPWPLV"
     gene            complement(4482..5000)
                     /locus_tag="QF838_07425"
     CDS             complement(4482..5000)
                     /locus_tag="QF838_07425"
                     /inference="COORDINATES: protein motif:HMM:NF022734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HJO60545.1"
                     /translation="MAGPINEFTQVTKFIPETFGEPGMRTFRINVDSESSTANIWLEK
                     EQLAELSMAMLQLAKETPEAKEDTVGPPTELEAASLTNLDFKAGRLAFGHNPETNMFI
                     VDAHDPEDDSEEPTVRIWLTRKRLNHFAKNGLEIVASGRPICPLCEKPINPDGHYCVR
                     RNGHAKVYPGEL"
     gene            5220..5411
                     /gene="rpsU"
                     /locus_tag="QF838_07430"
     CDS             5220..5411
                     /gene="rpsU"
                     /locus_tag="QF838_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HJO60546.1"
                     /translation="MAYVTLREGEEAEVLLKRFQTQMQRSGILRELRNRRFFRSKGEQ
                     ARLDQQRSIRRLRRRRTRR"
     gene            5482..6441
                     /locus_tag="QF838_07435"
     CDS             5482..6441
                     /locus_tag="QF838_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024891.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HJO60547.1"
                     /translation="MGILYVVSDTGRAGKTSFCASLASVLMDMGRKVTVSKAIFSEQD
                     EEDVDNYSSVLGSNFSKLSVRSFNVPDVGEEIKSVAEDVDTLLVEASNTLSRDEHIDL
                     VKNIGAKVIHVTKYDEAIGSIPFSTAFGQDLIGTVINFLTRYRGTKAHEVVIPSLRKS
                     GINVLGLIPENRTLLSLTVRQICENLEGKFFGGEEYGEDLVESFMVGGFGMDPGQYIF
                     SRQDKKAVVVRGDRPDVQMSALDTDMECFIMTGGFEPIEYVKYEANEEEVSIIIVNSD
                     TLETMDKIGTLQEHSEFDHKEKLARFKNLIDSNIDVEALKSVL"
     gene            complement(6589..8301)
                     /gene="ggt"
                     /locus_tag="QF838_07440"
     CDS             complement(6589..8301)
                     /gene="ggt"
                     /locus_tag="QF838_07440"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJO60548.1"
                     /translation="MVTNLASNATFTKSEAVSTNGMVATKDQLSTQAGLDMLKMGGNA
                     IDAGVAACLAVGVVEPESSGIGGGGYMTFQVGDEGGVIGFPMKGPLSGKPDLYELTGE
                     ASVGSFGWAGVKNDENIHGYKSIAVPGCVAGLLEAHSRFGKLPLSEVVAPATKIARDG
                     FHPEWFTLYKFGSLSSMLLRYPELGNTFMPSGSLPYGGINGPYVLKQPNLANALEAIG
                     KGGVDAFYRGELTEVLVEDIQKNGGFLTMEDFDQYKPFYWDKGLELNYHGKTVRVPRY
                     ASAGITSAMTLKILDGFDIPSLGHNSVDMLHLYISAARMAYADRFEYVADPEFADVPW
                     NGLVSDKYTRKRQKAIGDRAPESFLPGNPWIEEGREPKFVLESSKPRPDNGTTHLAVI
                     DGEGNAVSITNTIMSGFGSGIIPKGTGIVMNNGMMWYDPTPNRVNSISPGKYPLNNMT
                     PALVLGKNGVEVAVGASGGRRITNCVTSLIVKMVDFDMNPQTAIDAPRVDCSSPTTDV
                     GPELDPSIVAGLEAKGHSIRVLGDGFTQTGFAKFASPVAITKKGDEFRGGVDTFHAAH
                     AEGL"
     gene            complement(8395..9816)
                     /locus_tag="QF838_07445"
     CDS             complement(8395..9816)
                     /locus_tag="QF838_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60549.1"
                     /translation="MNKTESGLIPGPVIGEQDEENSSNHAAPKYLISPNNLNLDSDSP
                     SDKLLKKIRKPKGIPRKLHTFDAFHSRNYRFLWLATAIFSSGFWLQQVVVGWLAYEIT
                     ESPLLTSMALGLDALPILVGAPFGGLISDKFNKKILLSIIYSYQAVLMVGYSVVIAMG
                     NANIWNLFIFIFLVGISWVINDPARMSLLAVIIPKKSLINAFALNSMAFSVMRLLIPA
                     AGGFSLAIFGPAPLFIVEAILMFSAAAAIMMIQLDKDSSFDNSDRSAKQLLADLKSGV
                     TYVVNQKTIIGLTFMTVNMVILVMPFIHGLMPVYAAEVFEVGPQGLGLLLSAAGLGSL
                     IGTVILASCTQITRPGKILFVLLGFLGILMATLAITNIYSVAMIVIMLLSGSMMSYFS
                     ISGATVQGILPDQYRGRVTGIYMMAWGLMPVGSLLAGSIANVVGPQLSTLIGSSLIFV
                     SLIAATYCFKGVWKYRLEEDLSG"
     gene            complement(10312..11220)
                     /locus_tag="QF838_07450"
     CDS             complement(10312..11220)
                     /locus_tag="QF838_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024118.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO60550.1"
                     /translation="MSIKLDWLGCATFRLLIDDLTIFLDTYIDKVSAAPNVGISTAEI
                     KNADFIFIGHSHFDHIGGADVIAKNTGAEVIGSNESVRILIEAGVPKEQLRRAQGGER
                     YRLSDSVTVRVFPSLHSCIWIPNTFRPSTEESQTGHLNLTEDERWGPRKPDTLPSGTD
                     PNLIREIEDHRKETLGSSDIGGALCFLFETPQGSIFFQDTSGCWTGVVSDLRADAAIL
                     AMAGRPNIDGEPIQGSLTDYIGLMASMLKPKEIILGHHDDWMPPRTKDDSNSKSLQPV
                     IDRINLVRPGTLFRQPKYLDGTELLK"
     gene            11328..11696
                     /locus_tag="QF838_07455"
     CDS             11328..11696
                     /locus_tag="QF838_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HJO60551.1"
                     /translation="MTESYAANWTAVLVVAFVGALAVLLMFVASRVLAPKRHSAIKNS
                     PYECGIPASPHHWSQIQIRYYVFAILFLIFDVEAVFLFPWALVFLGAPPAVFYEMIIF
                     IGILLFGLAYVWRKGVLQWR"
     gene            11687..12328
                     /locus_tag="QF838_07460"
     CDS             11687..12328
                     /locus_tag="QF838_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HJO60552.1"
                     /translation="MEIDENPKIHIPKYDGLPPTEKPLGDGYEMGKGNNPADSVFNRV
                     LPNALTAKSDQLFALARKSSLWTLSFGLACCAIEMISAHASHHDLDRFGVVTWPSPRQ
                     ADVMIVAGTVVKKMADSIKLLYEQMPDPKWVIAMGSCATNGGPYYKSYSVVMGVDHLI
                     PVDVYVPGCPPRPEALIYGVMQLQEKIKNDANQSFEKESLNANEGEQQHIFKA"
     gene            12288..12833
                     /locus_tag="QF838_07465"
     CDS             12288..12833
                     /locus_tag="QF838_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012549.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HJO60553.1"
                     /translation="MPMKESSSISSKREIKAPIVREDLDQAGENLHKILKESISDFPG
                     SEISVSIDMVTVKIDSVQLAKICEFLKYTEKFGFDYLSCITVVDYEKDQEIFEIIYHL
                     ISIRLHHKMAIKVPLPSNDPTVPSVLGVWKSADWFEREAHDLFGIKFEGRENLPPLLL
                     YEGFEGYPGRKSYPFHDYGEW"
BASE COUNT         3522 a         2773 c         2703 g         3912 t
ORIGIN      
        1 cgatatgaga tgaatcgcct ggatacattg ttggcattct cgaggtcttt gtcactttgc
       61 aaaaagtcag gtggttctga tgaaatattt tcattagagc cgtttaaatt ttcgggcaat
      121 tttataattt tcaaggtaaa gaacttattt atatagcttt gtttacttct tcgtcactcc
      181 caaaatcatc cattggtacg attacatcat ccattggttc tggatttaat gctgctttaa
      241 ccatttcatt aatttcatca gttaaaaccg gattttcttt aaggaatgtt ttagcacttt
      301 ctcttccacg tcctatttgg acttccccaa aagaatagaa agccccactc tttttcacga
      361 tagagtgatc aacagctaaa tctagtaaat caccttccct acttatgcct tcgttgaaca
      421 taatgtcgaa ttcagctgtt ttaaatggag cagccagttt attttttaca actctcgccc
      481 gaacacgatt accgacaatg tcagttccct gctttaagga ttctattttt ctaagatcaa
      541 ttcgaacaga gctgtagaac ttaagtgctc gtccaccagg tgttgtttct ggacttcccc
      601 agaaaacacc aaccttttct ctaatttggt ttataaatat aacggcagtg tttgatttgc
      661 ttatagacga agttaatttc cgaagacctt gtgacatcag tcgtgcttgc aaccctatgt
      721 gagagtcgcc catatctcct tccagttcag cctttggcac catagcggca acactgtcaa
      781 tcacgaccac atccactgca ctgctgcgga caagatattc acatatctcc aaagcttgtt
      841 ctgcagtgtc gggttgagag actaggagat tttcggtgtc gacgccacaa cgagcagcat
      901 aactagggtc gagagcatgc tctgcatcaa tgtaggctgc agttcctccg tctctttgtg
      961 cagaggccat aacatgatat gtgagagttg atttgccgga agattcagct ccgtatactt
     1021 cagtaactcg acctttcggt attccgccca tcccgagacc agcatcgaga gagggtgagc
     1081 ctgttgaaat agaatctact gccacatctt gagcgtcacc cagcttcatt acagcacctt
     1141 tgccaaaatg tttgtctatt tggctgatgg ctagttctag agctgttgtt ttttcttttt
     1201 tgtccatcaa gaatctcctg aaaactttta gtgcatgagg cataaatatg aatatttaca
     1261 agaaggttag cacagcgatg ttacaaagta aatacaaatg ttctaaaaag aatcagtaaa
     1321 atagaacatg agtacgataa aagaatgtta agaaatcagc tctttgaggg tgtccccaat
     1381 ttcacctggt cctgggatta tagagactcc agcggcagag agggcagcct gtttttcctc
     1441 ggcttttccg gcagagccgg taatgatagc ccccgcatgg cccattcgtt ttcctggagg
     1501 ggcattttgg cctactatca aggcggcaat tggctttttg aactcacttt taatgtattc
     1561 cgcagcttct tgctcttttg ttccgccaat ctcccctatc ataaccacat tatctgtatc
     1621 aggatcatcc ttaaattttt ctaagatatc cacgaatgtc actccggata ctgggtcgcc
     1681 accaatccct acacatgtcg actggccaat gccgagttga gtcaattgtc cgactgcttc
     1741 atatgttagg gtgccacttc ttgagacgac accggtccta ccgggcaaat ggatatggcc
     1801 tggcatgatt cccattttga agttttctcc aggagaaatg attcctgggc agttgggtcc
     1861 aattagagtg acttccttat ctctaagata cctgaccacc tttatagtat cttggactgg
     1921 gatcccttcg gtgatacagg caattacttc tataccggca tctatggatt caacgatcgc
     1981 gtctgctgcg aatgcaggtg ggacaaaaat caaagaggca ttggcagaag tttcttttac
     2041 ggcctgttgc actgtgtcat agacaggaac cgtcgtatta aaattctgtc caccttttcc
     2101 gggagtcacc cctgctacca gatttgcacc atactcggcc gatctttgtg catgatagct
     2161 accttctttt ccagtaattc cttggactaa tagcttggtt gatttatcta cgagaatact
     2221 catgtatttg ttcctataag ctggagtaaa tgtatgtatt ggtaacttat gtttgggtta
     2281 tgctagcttt ttaatttcag ctgcggcctg ccctagggta tccactagga ttacatttag
     2341 gctggattgt gaaaggattt cccttccttc atctgaattt gttccaagca ttctcactac
     2401 cataggtctc actctattgg gttgttgttc agcggctaat aagaatcctc tcgcagcaat
     2461 gtcacagcgt aggattcccc caaagagatt aacaagaacc gccttaacgc tgtcatccga
     2521 taatactagg cctagggctt tggctacttt ttcctcgtct gcccctccgc ctatatccaa
     2581 aaaatttgag ggagatgcac cagctgaact ggtcacatcc atcgtggcca ttgcaaggcc
     2641 ggccccattg acaatgcagc cgacggtccc ttccaattta acataattta tatctgaaga
     2701 acgtgcttct acttctaggg ggtcttcttg agaaacatct ctcatttcct gcatgtcttt
     2761 gtgcctaaag atagcatcat catcaatgtc caattttgca tctgctgcca acacctcccc
     2821 gtctttcgta atgactagag ggtttatttc tagcaaggag gcgtctgttt ctataaaggc
     2881 ttggtatagg ttttgtacta agctggcgac gggacgaaca tgctcttgtt ctaggttaag
     2941 cccataagct atttttcgac cttgaaatgg ctgaagaccc aataccggat caatgggaat
     3001 ttgcagtatt ttttccgggg aagtttctgc tacctcttct atgttcatgc ctccagcttc
     3061 gctggcaatt acgactaccc cttttgaagc accatctata accattccta agtagagttc
     3121 gttttctatt tcaataccct cttctataag aacactgttg acaggcacac cttcaggacc
     3181 agtctgaaaa gtgaccagat ttgttcctat cattttttcc gctgcctgtt ccgcggcacc
     3241 aggggactcc acaattttaa cgccaccacc ctttcctctt cctcccgcgt gcacctgagc
     3301 cttaactacc acggtaccgc ccatctcaaa tgctgctgtt gcggcttggc ttccggttct
     3361 ggcaagccga ctttttggaa tgggtacccc gtattttgca aggatctctt ttgcctgata
     3421 ttcatggagt ttcatttttg tttttcctaa taagacacgc tatataaaag taactaaata
     3481 aaagtaacta aaacggcgga gggtgtggga ttcgaaccca cgatacccgt taggatatgc
     3541 cagttttcaa gactggtgca ttcgtccact ctgccaaccc tccatttata aaaatgactt
     3601 aggtatttta gtgcctagtg gaaagtatca gcaacttgtc tgtgagattc agcctaggct
     3661 atttttaaat aaatgttttc ttgtcacacc aatggccacg gtatgcttac attagggtcc
     3721 aaaattggca tgatgggatt ttcaataatt tgatccaatc ttgtgatcaa tgctgttatt
     3781 tcttcagtgc ttattagcaa tcctaacgat ttatcgtctt tattcaaata ggttcttaac
     3841 tttttcatat cggctatgag ggttggaggt atttgctgac cgcaatattc catcatcacg
     3901 gtccgcattt tgaatgcgct gtggaaggtt aaaccatgat cgatggacca tatattctta
     3961 aacccgtcaa gtaaacaatg cccagccttt ctgtctgcat tattaaccag taggtcaaat
     4021 actgctaaag ggaataaggt gtcaggagag tcatctctca tatcgaaata tgtgatacgc
     4081 gggtcacaat ccagatagag ttgcatggac ccaacaccat acgggccatc tcttattatg
     4141 gttagtggta tattaggcca acccaataat ttactaactt catatgctgc ttgctctctt
     4201 ttatacaggg tgccggcgga aaaatcccga agtggttttt ctccctcctt cggtttataa
     4261 atagccctca cacattgatt ttccccagca gtgacccaaa gcagaaaagt gtaattcgag
     4321 ccccatggca tagtatggcc gccgataact tccccgtctg tcaaaggtgc taaagcttcg
     4381 gtaggtccca gattcaatat ttccgcttca ggcaacggaa agttttttct tggatcaatt
     4441 ggatactgag cttgcataat ttctaaactc tagggactgg attagagttc ccctggataa
     4501 acttttgcat ggccatttct tctgacacag tagtgaccat cgggattgat aggtttttca
     4561 cataaaggac atattggtct tccagaggca actatttcta gcccgttttt ggcgaaatga
     4621 ttaagtcttt ttctagttag ccatattcta actgtcggtt cttcggaatc atcttccgga
     4681 tcgtgagcat cgactatgaa catatttgtc tcaggattat gaccaaaggc caatcgacct
     4741 gctttgaaat ccaaatttgt taaacttgcc gcttccaatt ccgtcggggg gcctacggta
     4801 tcttctttag cttccggggt ttccttcgcc aattggagca tcgccatcga tagttccgcc
     4861 agttgttctt tttccaacca tatatttgct gtgctacttt cactgtccac atttatcctg
     4921 aaagttctca tgcctggctc accaaatgtc tcaggtataa attttgttac ttgtgtgaac
     4981 tcattaatag gtccagccat cgatactcca aaaatgtggt ttggcgcatt ggaaaattag
     5041 gtatagatta aagtctaact ggaatagtag gtcttttgag ggctttgtag caacatcgat
     5101 tggttgccca ttaggcttct gtgccaaacg ggaatgtcct gtgtgttaca atttgcccct
     5161 gcgttgccgg atggcacgca tttttatttg gctgaaaatt agagactagg agaaaaagct
     5221 tggcatacgt tacccttcgc gaaggcgagg aggctgaagt acttcttaag aggttccaga
     5281 cccagatgca aagatctggt atcttaagag agttgaggaa cagaagattc ttccgttcta
     5341 aaggcgagca agctcggctt gaccagcagc gaagcatccg taggcttaga aggcgtcgaa
     5401 ctagacgata ataatttcta gctacgcatt caacaattgc taaacaggtt aatcaatttc
     5461 aacctatggt aggtgcgtca aatgggcatc ctttacgtcg tttcagatac cggtagagca
     5521 ggcaaaactt ctttctgtgc ctcattagcc agcgtcctaa tggatatggg ccgtaaggtc
     5581 actgtttcca aagccatttt ctcagaacag gacgaagaag acgtagataa ttactcttcc
     5641 gtgttgggct caaatttcag taaattgtct gtgcgatctt ttaatgtacc cgatgttggt
     5701 gaagagatta agtctgtagc tgaggatgtt gatactcttt tagtggaagc atcaaacact
     5761 ttgtcgagag atgagcatat tgatcttgtt aaaaacattg gagcaaaagt catacatgtt
     5821 acgaaatatg acgaggcgat tggatccatt ccattcagca ccgcttttgg tcaggattta
     5881 attggcacag tgataaattt tctgactaga tacaggggaa ctaaggccca cgaggtggta
     5941 attcctagtt taagaaaatc tggaataaat gttttggggt tgatacctga gaatagaacc
     6001 ttgctaagtc taactgtcag acaaatatgt gaaaatttgg agggcaaatt ttttggtggt
     6061 gaggaatacg gagaagattt ggtagaaagc tttatggtcg gtggttttgg aatggatcca
     6121 ggacaataca tatttagccg gcaagataag aaggccgtgg tggttcgggg agatagaccc
     6181 gatgtccaaa tgtctgcttt agacaccgat atggaatgtt ttatcatgac cggtggtttt
     6241 gaaccgatcg agtacgttaa atatgaagct aatgaagaag aggtttctat tatcattgtt
     6301 aactcggata cccttgaaac aatggataag ataggaacac tgcaggaaca ttcagaattt
     6361 gatcacaaag aaaaactagc cagatttaag aatcttatag atagtaatat cgatgttgaa
     6421 gcattgaagt cagtccttta attagcgtta gttattgttt ttccaattga gatcaaggcc
     6481 atgtttctca cttccgatta atatggcatg aggaacatta gtctaaccct ttcccaaaat
     6541 agcctggatt cctcttataa ataaaattgt tttagtggag aattcgattt ataggccctc
     6601 agcgtgggct gcgtggaatg tatcgacccc tcctctaaat tcatctccct tcttggtaat
     6661 agctaccggg cttgcgaatt ttgcaaaccc cgtttgagta aatccatcac cgagaaccct
     6721 aatgctgtga cctttagcct ctaagccggc aacaatactg gggtcgagtt ctgggccgac
     6781 atcagttgtt ggggagctgc aatccactct gggggcatca attgcagttt ggggattcat
     6841 gtcaaaatcg accattttca ctattagtga tgtgacacaa tttgttattc ttctcccacc
     6901 ggaagctcca acagcaactt ccacgccatt ttttcctaac actaaagccg gagtcatatt
     6961 gtttaagggg tattttccag gggagataga gttaacccgg tttggtgtag ggtcatacca
     7021 catcattccg ttattcataa caattccagt tcctttggga attattccgg atccaaaccc
     7081 tgacattatt gtgttagtaa tagaaacggc attgccttca ccgtcaatta ctgccaggtg
     7141 agttgtcccg ttatcggggc gtggcttgct actctctaag acgaattttg gttctctacc
     7201 ttcttcaatc catggatttc ccggaaggaa ggattcagga gcacggtctc ctattgcctt
     7261 ctgtcttttc cgggtgtatt tatcggaaac taaaccgttc catgggacat cggcgaattc
     7321 agggtctgcc acatattcaa acctgtcagc gtatgccatt ctggcggcac tgatgtacag
     7381 atgcaacatg tcgactgagt tatggcctaa actaggaata tcaaacccgt ctaggatctt
     7441 taaggtcata gcgctggtta taccggcgga agcatatctt gggactctca cggttttgcc
     7501 gtgataatta agttctaggc ctttgtccca atagaatggc ttgtactgat caaagtcttc
     7561 cattgtcaga aagccaccat tcttttgtat atcctcaacc aaaacttccg tcagttcccc
     7621 ccgataaaag gcatcaacgc ctcctttacc gattgcttcg agggcatttg ctaaatttgg
     7681 ttgttttaat acatagggac cgttgattcc accataggga agcgatccag atggcataaa
     7741 cgtgttacct aattctggat atcgcaaaag catgcttgaa agacttccaa atttatataa
     7801 ggtgaaccat tcgggatgaa atccatcacg agctatttta gtggctgggg ctacaacttc
     7861 ggataaaggc aattttccga atcgagaatg agcttcgagt aacccagcaa cacatcccgg
     7921 tacggcaatc gatttgtatc cgtgaatgtt ttcgtcattt ttgacgcctg cccaaccgaa
     7981 acttcccacg gatgcttcac ccgttagctc atataaatca ggcttaccag ataaaggtcc
     8041 tttcatagga aacccgatga caccaccttc gtcaccaact tgaaatgtca tataaccacc
     8101 accaccgata ccacttgatt ccggttctac tacacctaca gctaaacaag cggctacacc
     8161 tgcatcgata gcgttgccgc ccatcttaag catatccaac cccgcttggg tggataattg
     8221 gtctttagtt gctaccatac cgttagtgga cactgcttca cttttggtaa aggtggcatt
     8281 agaggctaag ttagtaacca tctattctct cctgccataa atttatagtt atctcttccg
     8341 tagcaggcgt catgatacat tagaatttgg cttagagtgc ccgattggaa gtacttatcc
     8401 tgacaagtct tcttccaaac ggtatttcca aacacctttg aaacaatacg tggctgcaat
     8461 caaactaaca aaaatcaggc ttgacccaat aagggttgat aattggggtc ccactacatt
     8521 tgctattgaa ccggccaata gacttccaac cggcatgagg ccccaagcca tcatatatat
     8581 tccggtcact cgacctctat attgatccgg aagaatgcct tgaactgtgg ctcccgaaat
     8641 agaaaaatag gacatcatag agccagataa aagcatgatg actatcatag ccactgaata
     8701 aatattagtt atcgctagcg ttgccattaa aatacctaga aatccaagta atacgaaaag
     8761 aatttttccg ggtcgagtaa tttgggtaca tgaggcgaga attacagttc ctataagtga
     8821 cccaagccca gccgcagaca agagtaatcc caacccttgc ggcccaactt caaagacttc
     8881 agcagcatag accggcatta aaccatggat aaacggcatc actagaatga ccatatttac
     8941 cgtcatgaaa gttaggccga ttatcgtttt ctggttaacc acgtaagtta ctccagactt
     9001 caaatctgcg agtaattgtt ttgcagatct atcggaatta tcaaaggaag agtctttatc
     9061 gagttgtatc atcataatgg cggcggccgc tgaaaacatg aggatggctt caacaataaa
     9121 taatggtgcc gggccgaata ttgcaagaga gaatccgccg gccgcaggaa taagtaatct
     9181 catcacactg aaagccatag aatttaatgc aaaagcgttt atgaggcttt ttttggggat
     9241 aatcacagca agcagtgaca tcctagctgg atcatttatt acccatgaaa ttcccacaag
     9301 aaatataaaa atgaataagt tccagatgtt ggcatttccc atggcaataa cgaccgaata
     9361 gccgaccatt aacactgctt gatatgaata aataatagaa agaagtatct ttttattaaa
     9421 cttatcgctt attaaccccc caaacggagc tcccactaaa attggtagag catcaagccc
     9481 aagggccata cttgtcagta atggggattc ggttatctca tatgccagcc aaccaaccac
     9541 cacttgttgg agccaaaacc cactgctgaa gatagccgta gccagccata aaaacctgta
     9601 attacgtgaa tgaaatgcat caaacgtgtg taattttctt ggaataccct taggcttccg
     9661 tatttttttt aaaagtttat ccgaaggaga atcggaatca aggttgagat tatttggtga
     9721 aatcaggtat ttaggagcgg catggtttga tgaattttct tcatcttgtt cacctatgac
     9781 aggcccaggt attagtccag actcggtctt attcaattat attgtcttgc acctcgccgc
     9841 tataggagtt tgtatgaatc tgaagattaa aatcgacgct atataagtct atagttttca
     9901 tgaattaaaa aacaagtatt tttgatctat ttaattaaat tgctaaacga ccattggaat
     9961 cttgaatgtc ccataattca gagggccatc ctaaagtttg cgcccttatt tttttcagta
    10021 gggcgtgctt tacccatgga agtacatact gaatattgga acaaaggttg tgcttcttta
    10081 aacttgacgc ggctagatga tccacgatgg tttgcttaaa tggtttgctt aaacggaaag
    10141 gtcgcctatg tatggatcaa ggggagtttg tatccttcac ttgcgagttg gatgcactga
    10201 cctttgctgc cttatagagt tagggtcgct gagtagaaac aaaggactga tggttggctg
    10261 accgatgcgg gggaatattt cttttcaagt tgtagctaat atggcatatc gttattttag
    10321 aagctctgtt ccatctaggt attttggttg gcggaatagg gttccgggcc gcacaagatt
    10381 tattctatca atcacaggct ggagggattt tgagtttgaa tcatcctttg ttctaggtgg
    10441 catccagtca tcatggtgcc ctaggatgat ttctttgggt tttaacatgc ttgccattag
    10501 gccaatatag tcagtaagtg aaccttgaat aggctcccca tcaatgttgg gccggccggc
    10561 catggctagg atagcagcat ctgcccttag gtcagagaca actccagtcc agcatccaga
    10621 agtatcttgg aagaaaatag atccctgcgg tgtttcaaat aagaaacata gtgccccacc
    10681 tatgtcgcta gaacctagag tttcttttct atggtcctct atctctctaa ttagatttgg
    10741 atccgttcca ctaggcaagg tatctggctt tcgaggtccc caacgttcgt cttcagtgag
    10801 attcagatgt cctgtttggc tttcttcagt gctaggtcga aaagtgtttg gaatccagat
    10861 gcaggaatgt aagcttggaa aaactctgac agtgactgaa tcgctaaggc ggtatctttc
    10921 acctccctga gctcttcgaa gttgttcttt aggaacgcct gcctcgatca gtattcgtac
    10981 actttcattg gaaccaatga cttctgcccc tgtatttttg gcaataacat ctgccccgcc
    11041 gatatggtcg aaatggctgt gccctatgaa aataaagtcc gcattcttta tttctgctgt
    11101 tgatatacct acatttggag ccgccgaaac tttgtcaatg taagtatcga gaaaaattgt
    11161 cagatcatct ataagtaaac gaaaagtagc acatccgagc cagtctaatt ttattgacat
    11221 attttctcac ctctaacaaa taaaatttac tagggacaca tatataaata tgctaaaatt
    11281 ggtttgtttt atgcccccat tattacagaa tttcttcggg tggtcctgtg actgaatcat
    11341 atgcagctaa ttggacagca gtattagtag ttgccttcgt gggagctttg gctgtgttat
    11401 tgatgtttgt agcatctaga gtattggctc ctaaacggca ctctgccata aaaaattccc
    11461 catacgagtg cggcataccc gcatccccac atcattggtc tcaaatccaa atacgttact
    11521 atgtttttgc gatattgttc ctgatatttg atgttgaagc tgttttccta tttccttggg
    11581 cgctagtatt cttgggcgct cctcctgcag ttttttatga aatgattatt tttattggta
    11641 tattgctgtt tgggttggct tacgtttgga gaaagggagt tttgcaatgg agatagacga
    11701 aaaccctaaa attcatattc cgaaatatga tgggcttcct ccgactgaaa aaccacttgg
    11761 tgatggttac gagatgggta aagggaataa ccctgcggat agcgttttca atagggttct
    11821 gcccaatgca ttgaccgcaa aatcggatca attatttgca ttagcaagaa aatcatcgct
    11881 ttggacttta tcttttgggt tagcatgctg tgcaattgaa atgataagtg cccatgcttc
    11941 acatcatgat cttgatagat ttggagtggt tacttggcct tcccctcgtc aagcggatgt
    12001 catgatagtt gccggcactg tggtaaaaaa gatggcagat tccataaaac ttttatacga
    12061 acaaatgcct gatcccaaat gggttattgc tatgggaagt tgtgcaacca atggaggacc
    12121 ctactataaa tcatattcgg tggttatggg agtagaccat ttaataccag ttgacgttta
    12181 cgttccaggt tgcccaccta gacctgaagc actaatttac ggtgttatgc aattacaaga
    12241 gaaaataaaa aatgatgcaa atcaatcctt cgaaaaagag tcactaaatg ccaatgaagg
    12301 agagcagcag catatcttca aagcgtgaaa ttaaagcgcc aatagtgcgg gaagacctcg
    12361 accaagcggg agagaatctc cacaaaatat taaaagaatc catttctgat tttccagggt
    12421 cagaaatatc ggtttctata gatatggtca cagttaaaat tgattcagtg cagctggcta
    12481 aaatttgtga atttctcaaa tatactgaaa aattcgggtt tgattattta tcctgtatca
    12541 ctgtagtaga ttatgaaaaa gatcaagaaa tatttgagat tatttatcat cttatctcta
    12601 tcaggctaca ccataaaatg gccattaaag tgccattacc gtcaaatgat cccacagtgc
    12661 cttcggtact gggtgtttgg aaatcagccg actggtttga aagagaagcc catgatttat
    12721 ttggcattaa atttgaaggt cgtgaaaatt tacccccatt gttattatat gagggatttg
    12781 agggttatcc aggaagaaaa agttatccat ttcatgatta tggtgaatgg taatggttag
    12841 taagtttact tatgcaagta catcaacagg ttagtgaata ttctgatgcg atagacctaa
    12901 tggtcaatat
//
LOCUS       DASCNS010000205         8381 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001574751, whole genome shotgun sequence.
ACCESSION   DASCNS010000205 DASCNS010000000
VERSION     DASCNS010000205.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001574751"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2194
                     /locus_tag="QF838_07470"
     CDS             <1..2194
                     /locus_tag="QF838_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HJO60554.1"
                     /translation="ISRRVARDESIEEIIASIIEVTTWELKAERGSLFLNDAATNELY
                     TRFAQGANLREIRIHNKSGIAGSVFQSGVGEIIHDAYNDDRFNSAVDQQTGYMTKNIL
                     CAPVKTLNGVIIGSAEVLNKKSGRFTKKDLELLEAMTEQASIALQSAQFVEQMQKSRD
                     EEKKLLDVVSEVTAEIDLPTMLSKVMGEATKMLNADRSSIFLNDPKTNELWLQIGDGL
                     ESTQIRFPNHLGIAGTVFTSGQTINIPHAYADLRFNPDFDKQTGYFTRSILCVPIINK
                     DRIILGVTQVLNKRGGPFSEEDEQRLKAFTAQISVGLENAQLFADVQNMKNYNDSILQ
                     SAASGVITLDEDEKIITCNEAGLRILSITGSEILQKPAVDFFIGENEWLMERINKVQD
                     EQQTDTFVDVSYQTDDQTLSLNGTVAPLISMDGKKLGVMVMLEDISTEKRMKSTMSRY
                     MDPGLADQLLEGDDNLGGEEKKATILFSDVRGFTTITEQLGAQGTVQLLNDYFSIMVD
                     SITNQGGMLDKFIGDAIMAAFGIPFTTDDDEDRALRASIDMITNLWEWNEVRAENGLP
                     PIDMGIGLNTDTVVSGNIGSPKRMDYTVIGDGVNLAARLESACKQYAARILVSEFTHS
                     NLKGVYRSREIDRVIVKGKTEPVGIYEVLDFHTAETFPNVMEVLNYFKDGLEKYRVGA
                     WDPAIKSFKEGQRLNPTDYITEMYIGRCQSLKADPPKDWDGVWVMTSK"
     gene            2273..2494
                     /locus_tag="QF838_07475"
     CDS             2273..2494
                     /locus_tag="QF838_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60555.1"
                     /translation="MLPHVTKFGIYFNSSEEKVVRITSPYWFPPEPEWVYVTKEVNAT
                     LLSIREMIKEQGLADQSDSVTWGRIPLLD"
     gene            2524..2712
                     /locus_tag="QF838_07480"
     CDS             2524..2712
                     /locus_tag="QF838_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60556.1"
                     /translation="MPHDNELKSDRLPEYVSKLSIDAEQWAILEPLVRSASNSVIMLD
                     QVLTVETESSAIFVLGKV"
     gene            2716..4122
                     /locus_tag="QF838_07485"
     CDS             2716..4122
                     /locus_tag="QF838_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HJO60557.1"
                     /translation="MDNLYELSISEASSLLKKKELSPVDLVASHLLRINETDNRLNSF
                     ITVLSDKSQKDALQAEKEILSGQWKGNLHGIPIGLKDLYYTKGIPTTVGSKIYKDFIP
                     DFNATVVDKLTEAGAVIIGKLQMHEFALGTTSHNPHYGPAKNPWNTECVTGGSSGGSA
                     SAVSGGQCMGALGSDTGGSIRIPSALCGIVGMKPTFGRVSRYGVFPLSSSLDTVGPMT
                     RTVKDNAIMLNAIAGHDINDSFSAKHSLSDYTSDIEKDISGIKIGNPQTYFYDVIDKE
                     VVCLMEKASEHFTDLGAKVEPAEVPILEHTLAISGTILLTEAAEIHSENIRTKAELIG
                     NDVKSRLQQGALTSGVDYVKSQRARTIYNQKIDDLFKTYDILVSPTVATGAPLLDDTS
                     VDINGVQELIAPLMARLTRPQNITGLPTISLPIGFTSKGLPVAMQLTAPPFQEARLYN
                     IANQYEKVSTFKNIIPSI"
     gene            4173..5093
                     /locus_tag="QF838_07490"
     CDS             4173..5093
                     /locus_tag="QF838_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60558.1"
                     /translation="MSTKERQGLSLYDAAREVGVSFEKEWKPESKSIEVNGMKFHYLE
                     WGDPANPVMVLLHGFAQQSHSWDFVALSFADRYRIIALDQRGHGDSDWASDGDYTPET
                     QQKDIEAIVTKLGLGEFVLMGLSMGGRNSFTYASSNPDQVKALIIVDAGPENVRAGTQ
                     NIRNFVEQEDELDSIDAFVDRVIKYNPRRDPIQIRGSIVNNLKQLPNGKWTWKYDKIL
                     RSQGRMGRPQDPEMTSRLWGYLENLQCPTMVVRGDKSDIIATETADRMIGRIPGGQLA
                     IIENAGHLVMGDNPSGFEKAVTAFIESIQK"
     gene            5215..5478
                     /gene="rpsO"
                     /locus_tag="QF838_07495"
     CDS             5215..5478
                     /gene="rpsO"
                     /locus_tag="QF838_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HJO60559.1"
                     /translation="MDRADKEAVIAEYRVHDNDTGSTESQVAILTSRINELTQHLREH
                     NHDESTRRGLLKLVGKRRRLLRYLNAEDVNRYRSLIARLGLRR"
     gene            5585..7924
                     /locus_tag="QF838_07500"
     CDS             5585..7924
                     /locus_tag="QF838_07500"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HJO60560.1"
                     /translation="MHGHDLELETGRLAQKAGGSILIKQGDNVVLATVTMSNPREGLD
                     FFPLTVDLEERHYAIGQIPGSFFRREGRPTTHAILTDRLIDRPIRPLFPKGFKNEVQV
                     IATTLSSDREIPLDIIALNGVSAALSISNIPFNGPIAATRIGYIDGEFVINPTYAQIE
                     ESLLDLVVAGSTDGVSMMEAGAKEVDEDVVFEAIQLAQSVNLEIISLQQDFADEIGTP
                     KSDFVPKGHDPEAVKQAREILGDRIYTAMSDAEDQEDMRNRLKVLEDELEESLSEEFD
                     SSVSAGAFEELLDEQFRVRILKDGVRPDGRGLREIRSLSAEVSILPRTHGTGLFNRGE
                     TQILGVTTLGSSGDAQKLDNLSPEVSKNFMLHYNFPPYSVGEARRVGSPGRREIGHGA
                     LAERALSAVLPSQEDFPYTIRIVCEALSSNGSTSMGSVCAGTLALMDAGVPITSPVAG
                     ISVGLITGEDGEYVTLTDIQGLEDHVGDMDFKVAGTSEGVTAIQLDIKVNSISFEVIK
                     EALSQAKEARSQVLDVITSAIPEVRSDVSPHAPRIQKIMVPTDKIGAVIGPGGKTIRA
                     IVEETNATVDIQDDGTVLIGSSNAEDAARAIQMVEDLTREIKVGEIFTGTVAKIASFG
                     AFVQILPGTDGMVHISELANYRVANVEDEVSVGDEVDVKVIGVDQSGKIKLSRKILLD
                     DSDGPSSSAPSPRKEIEKVEVNVGDIITGAVVNIAKFGAFVEIAPGTDGMVHISELEN
                     YRVAKVEDVVSVGEEITVEVIDVDDSGRIKLSRKALLTD"
     gene            7988..>8381
                     /locus_tag="QF838_07505"
     CDS             7988..>8381
                     /locus_tag="QF838_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HJO60561.1"
                     /translation="MADIKVVVNGVLGKMGQEVLNAVDKTQGMKPIGGSDIAANIDQI
                     PLPNGEGFIPVFQNIRDAVKGADVVVDFTNASGAKGVMEAAAPAGASCVIGSTGLSQE
                     DFEKAKSLAESNNTSIIIAPNFAMGAVLM"
BASE COUNT         2752 a         1634 c         1920 g         2075 t
ORIGIN      
        1 catctcaaga cgtgtcgcta gagacgaatc aatagaagaa ataatagcta gcataattga
       61 agtcacaact tgggaattga aggcagaaag aggatctttg tttttaaacg atgcagcaac
      121 gaacgagcta tacaccagat tcgcccaagg tgctaattta agagaaatac gtatacacaa
      181 caaaagcggg atagcgggat cagttttcca atcaggagtc ggcgagataa tccacgacgc
      241 atacaatgac gatcgtttta attctgcagt agatcagcag accggatata tgaccaagaa
      301 tattctatgc gccccggtca aaaccttaaa cggagtaatt attggaagcg cggaggtgct
      361 aaacaaaaaa tccggtcgct tcaccaagaa agatttggaa cttttagaag ctatgacgga
      421 gcaagcaagt atagcactgc aaagtgctca atttgtggaa caaatgcaga aaagtagaga
      481 tgaagagaaa aagcttcttg acgtggtctc ggaagtgact gcagagatag acttgccgac
      541 aatgctaagc aaggttatgg gggaagctac caaaatgctt aacgctgaca ggtcttcgat
      601 attccttaac gatccaaaaa ctaatgagct ttggttgcaa attggtgatg gcttggaaag
      661 tactcaaata aggtttccaa atcatctggg cattgcaggt accgttttta cttctggcca
      721 aactatcaac atccctcacg cttatgcaga tctaaggttt aacccagatt ttgataaaca
      781 aaccggatat ttcactaggt cgatattgtg cgtcccgatc ataaataagg acagaataat
      841 attaggtgtt acacaagtcc ttaacaaaag aggaggcccg tttagtgaag aagatgagca
      901 aagattaaaa gcttttaccg cacaaatttc tgtcggccta gaaaatgctc agctttttgc
      961 ggatgtacag aatatgaaaa actataacga tagcatctta caaagtgcag cctcaggcgt
     1021 gataactcta gatgaagatg aaaaaataat aacctgcaat gaagcaggtc ttagaatact
     1081 aagcattact gggtcagaaa tattacagaa acccgctgta gatttcttca tcggagaaaa
     1141 tgaatggtta atggagcgca tcaacaaagt acaggatgaa caacaaactg acacctttgt
     1201 agatgtatcc tatcaaacgg acgaccaaac attatcactc aatggcactg ttgctcccct
     1261 cataagcatg gatgggaaaa aacttggggt catggtgatg ctagaagaca tcagtacaga
     1321 aaagagaatg aagtccacta tgtccagata catggaccca ggtctggcgg accagttgct
     1381 tgagggagat gataatttag gcggtgaaga aaaaaaagca acaattctat tttcagatgt
     1441 cagagggttt acaactatca ccgagcaatt gggagcccaa ggaaccgttc aattactaaa
     1501 cgactatttt tccatcatgg tggattccat aacgaaccaa ggggggatgt tagataaatt
     1561 tattggggat gccataatgg cagccttcgg aattccattc actactgatg atgacgaaga
     1621 tagagcactg agagcctcaa tcgacatgat aaccaacctt tgggaatgga atgaggtgcg
     1681 ggccgaaaat gggctaccac caattgacat ggggatagga ttaaacactg acacagttgt
     1741 atctggaaat attggttccc ccaaaagaat ggattacaca gttataggcg acggggtgaa
     1801 tttggcagcc agattagaat cggcctgcaa acagtacgcc gctcgcatat tggtgagtga
     1861 gtttacgcat tccaatctaa aaggcgtata taggtctagg gaaatcgaca gggtgattgt
     1921 gaaaggtaaa acagagcctg taggaattta tgaggtacta gatttccata ccgcagaaac
     1981 attcccaaat gtaatggaag tattgaacta cttcaaagat ggattagaaa aatatagggt
     2041 tggtgcctgg gatcctgcta taaaatcatt taaagaaggc caaaggttaa atccgacaga
     2101 ttatattact gaaatgtata taggtcgttg ccaaagctta aaagcagacc ctccgaaaga
     2161 ttgggatggg gtttgggtga tgacatcaaa ataaataata aaatcactaa gcccacttga
     2221 acgaaattat gtcctatgct aagtttggct gacttttaac gggagatttg ctgtgctacc
     2281 acatgtaact aaatttggta tttattttaa ttcttcggag gagaaagtcg tcagaattac
     2341 ttctccatac tggttccctc ctgagcctga atgggtctat gtaaccaagg aagtaaacgc
     2401 cactctcttg agtattcggg aaatgataaa agagcaagga ttagctgacc aaagtgattc
     2461 tgtgacttgg ggtcgcatac ctttgttaga ttagcttcag gaaatcttcg gagcccatat
     2521 taaatgccac atgataacga gctaaaatca gatagactcc cagaatatgt ctctaaatta
     2581 tcgatagatg ctgaacaatg ggccatctta gaacctttgg ttagatctgc ctcaaattct
     2641 gtcataatgc tggaccaagt gctaacagta gaaactgagt catcagctat ttttgtcttg
     2701 ggaaaggttt aagccttgga caatttatac gaactttcca ttagtgaagc ttcaagctta
     2761 ctgaaaaaaa aagaactctc ccctgtagac ctcgtagcct cacatcttct tcgtataaat
     2821 gagacagata accggctgaa ctcctttata accgtcttaa gtgacaaatc ccaaaaagat
     2881 gcactccagg cagaaaaaga aattttgtca gggcaatgga agggaaatct acacggaatc
     2941 cctatcggac tcaaagatct ttattacacc aaaggcatac cgacaacagt agggtccaaa
     3001 atatataaag acttcatccc cgattttaac gctaccgtgg tggacaaatt aactgaggca
     3061 ggggccgtca tcatcggtaa gctacaaatg catgagtttg cactggggac aaccagccat
     3121 aaccctcatt atgggccagc caagaaccca tggaacactg aatgtgttac tggtggctct
     3181 agcggaggat cagcttccgc agtctccggc ggccaatgca tgggggcttt aggtagtgat
     3241 acggggggat caatacggat accttcggct ttatgtggga ttgttggtat gaaacccaca
     3301 tttgggagag tctcaagata tggcgtgttc cccttgagca gttctttgga tactgtaggt
     3361 cctatgacaa gaacagtgaa agataatgcc ataatgctca acgctattgc aggacatgat
     3421 ataaatgatt ctttctctgc aaaacacagc ctatcggatt acacatccga tattgaaaaa
     3481 gacattagtg ggataaaaat agggaaccct caaacatatt tctacgatgt tattgataaa
     3541 gaagtagtct gcttgatgga aaaagcctcc gagcatttta cggatttggg ggccaaagtc
     3601 gaacctgctg aagtacctat acttgaacat accctagcta tatccggaac aatacttcta
     3661 acagaggcag ccgagataca ttctgaaaat attagaacta aagcagaatt aattggcaat
     3721 gatgttaaat ctcgattgca gcaaggcgct ctgacatcag gcgtggatta tgtaaaaagt
     3781 caaagggcaa gaactatata taatcaaaaa atagacgact tatttaaaac ttatgacatt
     3841 ttagtttctc ctacagtggc cacaggagca cccctattag acgatacatc tgtagatatc
     3901 aacggagtcc aggaactcat agcaccatta atggcaagac taactcgccc acaaaacata
     3961 acaggacttc ctaccatttc acttcctatt ggtttcacat caaaggggtt accggtggca
     4021 atgcaactta ctgcacctcc attccaagag gctcgactct ataacatagc caatcaatac
     4081 gaaaaagtat caaccttcaa aaatataatt ccatcaatct agaaatctgt cacaatatcc
     4141 aaataaaata atggtctgga gaagataagt ttatgagcac taaagaacgt caaggattat
     4201 ctctatatga tgctgcaagg gaagtaggcg taagttttga aaaagaatgg aagcctgaaa
     4261 gcaaatcaat agaagtgaac ggaatgaaat tccattacct tgaatggggc gaccctgcca
     4321 atcccgtcat ggtactgctt catggctttg cccaacaatc acattcttgg gacttcgttg
     4381 ccctgtcatt cgcagacaga tatcgaataa tagcattgga tcaaagagga catggcgaca
     4441 gtgattgggc gtcggacgga gactacactc cagaaacaca acaaaaagac atagaggcaa
     4501 tagtaaccaa actaggcctc ggcgaattcg ttctgatggg actttcgatg ggtggaagaa
     4561 actcattcac ctatgcctcc tctaaccccg accaggtaaa agctttaata attgtggatg
     4621 ctggacctga aaatgtcagg gcaggaactc agaacataag aaacttcgtg gagcaggagg
     4681 acgaacttga ctctatcgac gcctttgtcg acagggtaat caaatacaat cctcggaggg
     4741 atccaataca aatcagagga agcatagtta acaatctaaa acaacttcct aacggtaaat
     4801 ggacatggaa atatgacaaa atcctaagat cccaaggaag aatggggcgt ccccaagacc
     4861 cagaaatgac tagcagacta tggggttatt tagagaactt gcagtgcccc accatggttg
     4921 ttagaggaga caagagcgac attatagcta ctgaaactgc tgaccgaatg attggaagaa
     4981 ttccgggtgg tcaattagcg ataatagaaa atgccggaca ccttgtcatg ggtgataacc
     5041 catccgggtt tgaaaaggct gtaactgcct tcatagaatc gattcaaaaa taatatcttt
     5101 ataatttaaa tccaatgttg aattataaat tgctacaccg aagattgggt gttacacttt
     5161 ttacaaactt aaattagtta gataacaaga aagagagaaa tccaggagaa cggattggat
     5221 agggctgaca aggaagccgt tattgcggaa taccgcgttc acgacaacga tacagggtcg
     5281 actgagtcgc aagtagcgat actgacttca cgtatcaatg aattgaccca gcacttaaga
     5341 gagcacaatc acgacgaatc cacaagaaga ggtttgctga aacttgtagg aaagcgtcgg
     5401 aggcttttac gctatctgaa tgcagaagac gtaaacagat acaggtcgtt aatagccaga
     5461 ttgggtctta ggcggtagta acttacacat cactcccccc ccgattattg cacatctccc
     5521 atctgaacct ctggtactac tccaggagta aatagacatt tcattacata caataacccg
     5581 taatatacac ggacacgatt tagaattaga aacggggcgg ttggctcaaa aagcaggcgg
     5641 ctcgatattg atcaagcaag gtgacaatgt ggttctagcc acggtcacaa tgagtaatcc
     5701 tcgagaagga ttagattttt ttcccctcac agtggatctt gaagaaagac actacgctat
     5761 tggccaaata cccggaagtt tttttagacg ggaaggcagg cctacaaccc acgcaatact
     5821 cactgacagg ctaatagaca ggccaataag gcctttattc cccaagggat ttaaaaacga
     5881 ggtccaagtt atagcaacaa cactgtccag tgacagagaa atacccttag atatcatagc
     5941 cttaaacggg gtttccgctg ccttgagcat atcgaacatc ccatttaatg gacctattgc
     6001 agcaacccga atcgggtata ttgatggtga atttgtaatt aaccctacat atgctcagat
     6061 tgaagaaagc ctcttagatc ttgtggttgc aggatctacc gatggcgtat caatgatgga
     6121 agccggggca aaagaggtag atgaggatgt cgtgtttgag gctatccaac tagcacagtc
     6181 ggtaaattta gagattatct ccctacaaca agatttcgct gatgaaattg gtaccccaaa
     6241 atcagatttt gtaccaaaag gccatgatcc tgaagctgtg aaacaagcaa gagaaatttt
     6301 gggagaccgt atttacacgg caatgtcaga cgcagaagat caagaagata tgaggaatcg
     6361 tttaaaagtt ttagaagacg aattggaaga atccctgtca gaggaatttg attccagtgt
     6421 ttcagctgga gcctttgagg aactgctaga tgaacaattc agggtcagaa tacttaaaga
     6481 cggagttaga ccggatggga gaggtttgag agaaatacga tctctttcag cggaggtttc
     6541 aattttacct cgtactcatg gcacaggact atttaataga ggtgaaacac aaatactggg
     6601 tgtaaccacc ctcgggtctt caggcgacgc acagaaacta gacaatctaa gcccagaagt
     6661 atccaaaaac ttcatgctcc actataactt tcctccttat tctgtcggag aagcgagaag
     6721 agttggatcc cctggccgta gagagatcgg gcatggagct ttagcagaaa gagccttgag
     6781 tgctgtttta ccttcccaag aagatttccc ctacacaata agaattgtat gtgaagctct
     6841 tagctcaaat ggtagtacat ccatggggag tgtttgcgca ggaactttag ctctaatgga
     6901 tgctggagtg ccgattacat ccccggtagc tggaatatct gtcgggctta ttactggtga
     6961 agatggtgaa tatgtaacct taaccgacat acaaggatta gaagaccatg ttggagatat
     7021 ggatttcaaa gttgcaggta cttctgaggg agttacagcg atacaacttg acataaaggt
     7081 aaatagcatc agttttgagg taataaaaga ggccctctca caggcaaaag aagccaggtc
     7141 acaagtattg gacgttataa cgtctgctat acctgaagtc agatctgatg tcagtcctca
     7201 tgctcccaga atccaaaaaa tcatggttcc tacagataaa ataggagcag taattggccc
     7261 aggcggcaaa acaatcagag cgatagttga agaaaccaac gctaccgtcg acatacaaga
     7321 tgatggaacg gtccttatcg ggtcatctaa tgcagaagat gcggcgagag caatacagat
     7381 ggtggaagat cttacgagag agatcaaagt aggagaaatc tttaccggaa ccgtagctaa
     7441 aatagccagt tttggagcct tcgttcaaat attacctggt acagatggta tggtccacat
     7501 aagtgaactc gccaattacc gggttgcaaa tgtagaagat gaagtatcag tcggagatga
     7561 agtggatgtg aaagtcatag gggttgacca gtcaggaaaa attaaattgt ccagaaaaat
     7621 acttctagat gatagtgatg gcccgtcgtc ttctgctcca agtcccagga aagaaattga
     7681 aaaggttgag gtaaatgttg gggacatcat aaccggagca gtagtgaaca ttgcgaaatt
     7741 cggggccttc gttgaaatag cacctggcac agacggaatg gttcatataa gtgaattaga
     7801 aaattacaga gtggccaaag tcgaagacgt tgtaagtgtg ggagaggaaa ttacggtgga
     7861 ggtcattgat gtagacgatt ccggtcgtat aaaactttct cgtaaggccc ttttgactga
     7921 ctaaaacttc tcaattgttg gaagttaggt tttacagagc aaccaatgtg ataaaaggag
     7981 ttccaccatg gctgatatta aagtcgtcgt taacggagtc ctaggaaaga tgggtcagga
     8041 ggttttaaat gccgtggata aaacccaagg tatgaaacct attggtggtt cagatattgc
     8101 cgccaatatt gatcaaatac cattgcctaa tggagaagga ttcataccgg tatttcaaaa
     8161 tataagagat gcggtcaaag gagctgacgt tgttgtggat ttcacaaacg catctggcgc
     8221 aaaaggtgta atggaagccg ctgcaccagc tggagccagt tgtgtcattg gttcaactgg
     8281 tctatctcaa gaggattttg agaaggctaa atcgttagct gagtctaata acacaagcat
     8341 aatcattgct cctaatttcg ctatgggagc ggtattgatg t
//
LOCUS       DASCNS010000206         9774 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001577940, whole genome shotgun sequence.
ACCESSION   DASCNS010000206 DASCNS010000000
VERSION     DASCNS010000206.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001577940"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="QF838_07510"
     CDS             <1..444
                     /locus_tag="QF838_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HJO60562.1"
                     /translation="LLAEVNQSRTKPLLVKMPPYKSDEGDHEKTLNLGRICLEEKVES
                     LTVSNTIPIAHDGLAVGHGGLSGNPLFENTLAMVKTYHSEFGDSIEINACGGISNGYQ
                     AYEALKAGASSIQILTALIYQGPRVVQKINKELSELITNKGNPKN"
     gene            complement(434..1366)
                     /gene="rbsK"
                     /locus_tag="QF838_07515"
     CDS             complement(434..1366)
                     /gene="rbsK"
                     /locus_tag="QF838_07515"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HJO60563.1"
                     /translation="MPASHIVVLGSLNVDLVTAVEELPKNGQTIFSDSFVIENGGKGA
                     NQAVAASRLGANVAMIGRVGADVFSEQVLTDLKSEGVDVSRITKDVNHSTGIALITVD
                     SKSENTIVVSSGANMSCGEVELDSLKDCIAGASCLLLQNEVPLEVNLEAADIARDNNV
                     PVIWDPAPYVRDIDELIRKVDFLTPNQTEAGLLTSRELKDVKSVHTALLKMKDITEAI
                     CIITMGREGLFLLSGNELKHVPSSTVISVDSVAAGDAFTAGLAVAISEGLTLIEAAEL
                     GSAAGALATTRRGAQIAMPFRAEVDSLMNKTTNS"
     gene            complement(1372..2181)
                     /locus_tag="QF838_07520"
     CDS             complement(1372..2181)
                     /locus_tag="QF838_07520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HJO60564.1"
                     /translation="MKYKIIALDIDGTLKGDSSSISPYMLEILDECSKRGALVSVATG
                     RSLKSAMIFLRQAPMIETIVSFQGALVSFDRGEKKVWETFLSAQQVSLSVRLLAQSNV
                     EVVGYAGDDVYVEEMSDWAEAYGERNGVKIFLVDSLEEIGRDLYRVLGVGDPEVVAEL
                     ESDLKSKYSGEVYATRSLPHFCEILSIDAGKDKALTWLCSQTNVMVEEVIAFGNGFND
                     VEMLKWAGRGVAMAGGESVVFEVCNDLAKSPDEDGVAVYLEDLLSKNQIGL"
     gene            2371..3183
                     /locus_tag="QF838_07525"
     CDS             2371..3183
                     /locus_tag="QF838_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013530.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60565.1"
                     /translation="MSGRKVLITGLAGKIGGIIRSNLTLKYALSGLDLQKVPEFPTTV
                     ANLSNLDEILPAFEGIDTVVHLAADPNHQGSWETNLENNIIGTRNVYEAARRSGVNRI
                     VFASSNHALGFYPLRDNPYKQIYEGEFREVRHPVIPLATNLVRPDGYYGVSKAFGESL
                     GSYFHDEYGMSVICLRIGWVMEPDDPTFAPSALSLWMSHKDTANLIDCSIDAPESVGF
                     AIVYGMSDNTYGIWDMEQGRKIIGFMPEDNAGTQWAPSKDRSSVMKSGDPQE"
     gene            3208..4293
                     /locus_tag="QF838_07530"
     CDS             3208..4293
                     /locus_tag="QF838_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60566.1"
                     /translation="MKIQRYETGIVSLPREEGPLSGGMGSKAEFITIKIITDDGIEGI
                     GYSGFASSVMVKALKETVDALLEQIIGEDPRRVEHINTYLRNLAGGGAPAGLVTRGVS
                     GIDVALWDIKGKAASEPLHRLLGGFRDTVPTYASGYLWRNFDLSRLENTSKELVAQGF
                     KAMKFRMGAEDSAQKEIARMETMRNSVGPDITVMLDINQGWDVNTAISIGRELAQYDL
                     FWLEDPINHQDFEGLSRIADALDTPVAAGEYHYGIEPFRTQLERRSIDIVMVDLLRAG
                     GITQWMKAAHLAEAYNLPVVTHLATEVLMHCLAACPNGLYVEHMPWTFAMFNEEPEVV
                     GGEIVLPQSPGLGLSFNDDNLKKYAVS"
     gene            4412..6691
                     /locus_tag="QF838_07535"
     CDS             4412..6691
                     /locus_tag="QF838_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HJO60567.1"
                     /translation="MATQTPKNYSAIGKRPVRHDGADKVTGKAQYGADLNLPRMLFGK
                     VLRSPHAHARIKSIDLSAALAHPEVKAIVTSSDLSPLPDKPAEVGEDLYANMKYVRDR
                     ILASDKVLFKGHPVAAIAANGLHEAEELLSLIKVEYEVLESVTDVESAMSENAPILHG
                     TIRFASMSGDEVHGSNIASHDQYILGDINEGFKKADLTIEREYRTKTVHQGYIEPQNG
                     TASWRSDGHVTIWCSSQGHFGIRDQVSTILGLPVSKVTIVPMEIGGGFGGKLPCYLEP
                     LAALLSKKTGTPVKMFMTRSEVIEASGPTSGSFVRVKIGVTNDGKITAAKGYLAFEAG
                     AFPGSPIGGATACMLSPYDIENLQLDGYDVVDNKPKTTAYRGPGAPIGALAVETVIDE
                     IAEKLKIDPVDLRLLNAAKEGTRRSDGVVNGKIGMIELSEIVKNHDHYQTPLEEVEGK
                     LRGRGIAFGYWRNNTGPAACVATVTPDGTVNLSEGSVDVGGSRTAIAQQLAEVLSIPV
                     EDVNPQVADTDSIGYTSVTGGSGVAFKSGWAAFEAAQEVKKEMLKRASLVWDAKEDEV
                     EYADGIAQHKSDPELKLTFKEIAAASNGTGGPIVGSAGVNPRGVGGSFAAMLVDVEVD
                     EETGKTTILRCTPFTDVGKAIHPAYVEGQIQGGTAQGIGWALNEEYFMSDDGKVMNAS
                     LLDYRMPTSLDLPMIDPVVVEVFNPGHPFGVRGVGESPLVPPLAAMANALYHATGVRM
                     RELPMSPTAVKKAIDNAKI"
     gene            6755..8356
                     /gene="groL"
                     /locus_tag="QF838_07540"
     CDS             6755..8356
                     /gene="groL"
                     /locus_tag="QF838_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922249.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HJO60568.1"
                     /translation="MPKQFQFKDNARTQLMEGIDLMARTVGITLGPSGRNVILEKEFG
                     PPQVCSDGVTIAKEIELLEPFHNMGAQLLKEAASKTNDDVGDGTTTSTILAQAILKNG
                     FKNIAAGADPMALKRGIDQAVDVITKELINISKDVTGDEQISQVAVLSAHDEKMGQLI
                     GQVMSKVGKDGVVTVEESRGQDYEVEYVEGMEIDRGYLSPYFVTDQDRMVTELNEPYI
                     LITSEKITSLEELLPFLEKFSAVGRELVLIAEDIEEQALATLVVNKMKGTLNCLGVKA
                     PAFGDRRKAILEDMSILFGGTVISSETGRTLDSIEISDLGRCRRVISTKDSTTFVEGS
                     GEASLIDARVSNIKSQAQETKSDYDREKLEERAAKLSGGVSIIKVGAATEVELKEKKQ
                     RVEDALSATRAAMEEGILPGGGTSLIRIAADLRENYSDSSDEATGARIVLDSVDAPVV
                     LLIQNAGFSGQVVLDAIKKGSGDYGFDAENDRYGSMYEFGVIDPVKVTRSALENAASI
                     AGMVLTTESLITELDPPKLPAPFDD"
     gene            8410..9408
                     /locus_tag="QF838_07545"
     CDS             8410..9408
                     /locus_tag="QF838_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJO60569.1"
                     /translation="MSTDKENENPIDPDVTISVEIVSKSFGPHKAVSNLSFSIKRGEI
                     VGFLGPNGAGKTTTMRLLTSYYTPDTGKILINGIDNSENDLETRKSIGYLAENNPLYE
                     DLLVSEYLDFIADLRGLSGKERTRNLEQTVEEASLQEVFYRPISELSKGYHQRVGLAG
                     AIIHRPSILILDEPTEGLDPNQRLSMRDLIKSLGQDRTVLVTTHVMQEVENTCERVLL
                     INRGQLTADSPVDEIFQLTPGLRKIYVEAEGNQIESGLSSLDSVKSVERLDPIDSRKR
                     YIVNLSGSEDPRPAIFKIATNNGWILWELHEERMRLEDVFHTLTSENEAERSLLGS"
     gene            9405..>9774
                     /locus_tag="QF838_07550"
     CDS             9405..>9774
                     /locus_tag="QF838_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024091.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJO60570.1"
                     /translation="MKSPFLALIKKDLKGYFDQPTGYILIVVFVSMLAWSFFQSALLI
                     GEASLRPLFTVDFAIDKPSLPWLLALFIPAATMRLLSEEQRDGTLETLLTQPIRGWIV
                     LLAKFTSGLLFVSIAIVATIG"
BASE COUNT         2922 a         2072 c         2264 g         2516 t
ORIGIN      
        1 ctgttagctg aagtaaacca aagtagaact aaacctctcc ttgtaaaaat gcctccatat
       61 aagtccgatg aaggtgatca tgaaaaaacc ctcaatttgg gtcgcatttg cctagaggaa
      121 aaggtggaat ctctgacagt ttctaacacg attccaattg cgcatgatgg tcttgctgta
      181 ggtcatggag ggctgagcgg aaacccttta tttgaaaata cgttggcgat ggttaaaact
      241 taccactcag aattcggtga tagtatagaa attaatgcct gtggaggaat ttcaaacggc
      301 tatcaagcat atgaggcctt aaaggcaggg gctagttcca ttcagattct cacagctctt
      361 atttatcaag ggcctagggt cgtccaaaag atcaacaagg aactatctga actcattaca
      421 aataaaggta atcctaagaa ttagttgttt tgttcatcaa tgagtctact tctgctctga
      481 agggcatagc aatttgagca cctcttctcg ttgttgcaag tgctccggct gcggaaccca
      541 actcggccgc ttcaataagc gttaaacctt cagatattgc gacagccaaa ccggcggtaa
      601 acgcatctcc tgcagctaca gagtcaacac ttatcacagt ggaactaggc acatgtttca
      661 attcgttccc tgataataaa aagagtcctt cacggcccat ggtgataatg catatcgctt
      721 cagtaatatc cttcattttc aataaggctg tatgaacaga tttcacatcc tttaattcac
      781 gacttgtgag taaccctgct tccgtttggt tcggggtgag aaaatcgact tttctgataa
      841 gttcatcaat atccctaaca tacggtgccg gatcccaaat tactggaacg ttattatccc
      901 gtgctatatc cgccgcctct aggttaactt caaggggcac ttcgttttga agcagcaggc
      961 agctcgctcc ggcgatacag tccttaaggg aatccagctc cacttcacca caactcatat
     1021 tggcaccaga tgaaacaaca attgtatttt cagactttga atctacagtt atcaaggcta
     1081 tacctgtact gtgatttaca tctttcgtta tacggctgac atccaccccc tcagatttca
     1141 agtcagttaa gacctgttct gagaaaacat cagcccccac tcttccgatc atcgccacgt
     1201 ttgcccctaa tctcgaagct gcaactgctt gattggcacc tttgccacca ttttcaataa
     1261 caaatgaatc tgaaaaaata gtttgaccat tcttaggcaa ctcttccaca gcagtaacta
     1321 aatcgacatt caggctgccc aatacaacga tatgtgaggc aggcatgaat tctataaccc
     1381 tatctgattc tttgataaaa gatcttctaa atacacagct actccatcct catctgggct
     1441 tttggccagg tcattacata cttcaaaaac tacagattct cccccagcca ttgcaacccc
     1501 tctaccggcc cacttaagca tttccacgtc attgaagcca tttccaaaag ctattacttc
     1561 ttcaaccatc acattagtct gggaacaaag ccatgtcaaa gctttatcct taccagcatc
     1621 gatactaagt atttcgcaaa aatgcggtaa tgagcgggtg gcatacacct caccagaata
     1681 tttcgatttt agatcggatt ctaactccgc cactacttct gggtctccga ctcctaagac
     1741 acgatacaga tccctaccga tttcttccaa agaatccact agaaatattt tcactccgtt
     1801 cctttctccg tatgcctcag cccagtcact catttcttct acgtagacat cgtctcctgc
     1861 ataacccact acctccacgt tagattgagc tagcaatcgc accgaaaggg acacttgctg
     1921 agctgaaagg aaagtctccc aaaccttttt ttctcctcta tcgaaactga ccaacgcccc
     1981 ttggaacgac acgattgtct ctatcatggg tgcttgccgg aggaaaatca tggcagattt
     2041 taggctccta cctgtggcga ctgataccaa agcacctcgt ttggaacatt catctaatat
     2101 ttccagcatg taaggggaaa tagaagaaga gtctcctttc agtgtcccgt caatatccaa
     2161 ggcaataatc ttatatttca aatccttaac ttctccaggg ctgagatagg ctgtgctaaa
     2221 atcaccgtga ttttcgttac cccacaacca ggtagattta tgcgcatcac acccagacta
     2281 atactatggg cctgcattgg ccctttggta gtttatttga taatacaggc ctttgggggt
     2341 aaataaaaaa tagccgtagg agaaacacca ttgagtggaa gaaaagtcct gataacggga
     2401 ttagcaggga aaataggtgg aataatccgg tcaaacctaa cgttaaaata cgctttgagt
     2461 ggtcttgacc ttcagaaagt gccagaattt cctactactg tcgctaatct ttcaaatctg
     2521 gatgaaatac tgccggcatt tgaaggtatt gacacagttg ttcatctggc agcagatccg
     2581 aatcatcaag gtagttggga aaccaatctg gaaaacaaca taattggtac gcgtaacgta
     2641 tatgaagccg cccgccgatc aggagtgaac cggatcgttt ttgctagcag caatcatgcc
     2701 ttgggatttt atcctctaag ggataatccc tataaacaaa tctatgaggg agaattccga
     2761 gaggtacgtc acccagttat tcccttggcc accaatcttg tacgaccaga cggttattat
     2821 ggggtaagta aggcattcgg ggaatcctta ggtagctatt ttcatgacga atatggaatg
     2881 tcggtcatat gtctgagaat aggttgggta atggagccag acgacccgac ctttgctccg
     2941 tccgccttgt ctttgtggat gagccataaa gacaccgcaa acctgatcga ctgcagcata
     3001 gatgcgccag agtctgtagg atttgctatc gtctacggaa tgtcagataa cacttatgga
     3061 atatgggata tggaacaagg gcggaagata ataggtttta tgcctgaaga taacgcaggc
     3121 acacaatggg ctccatcaaa agatcggtct tcagtcatga aatctggaga cccacaagaa
     3181 tgattgattg caagaggaga aaaagaaatg aaaatacaaa gatatgagac aggtattgtc
     3241 agtctgccta gagaggaagg tcctctgtca ggagggatgg gcagtaaggc agaattcata
     3301 acgataaaga tcatcacgga tgatggtatc gaaggaatag gttattcggg gttcgcgagt
     3361 tcagtaatgg tcaaggcctt aaaggaaacc gttgatgcat tgcttgaaca gattatagga
     3421 gaagacccca ggcgagtaga acatatcaat acttacctga gaaatctggc gggaggcgga
     3481 gccccagctg gccttgtaac acggggagtc tcaggaattg atgttgccct atgggatatt
     3541 aaaggcaaag ctgcatcaga acctctccac agattgctag gagggttcag agacactgtt
     3601 cccacttatg caagtggtta tttatggcga aattttgact tgagccggtt agaaaataca
     3661 agtaaagagc tcgtagctca gggcttcaag gctatgaaat ttcgtatggg agctgaagat
     3721 agtgcccaaa aagagatagc aagaatggaa accatgagaa attcagtggg tccagacatt
     3781 acagtgatgc tggatatcaa ccagggttgg gacgttaaca ccgccatttc cataggaagg
     3841 gaactcgcac aatatgatct tttttggcta gaagatccaa taaatcacca agattttgaa
     3901 ggtttgtctc gtatcgccga tgccttggac acaccagttg cagcgggaga ataccactat
     3961 ggtatcgaac catttaggac tcaactagaa cgtcgatcca tcgatatagt catggtcgat
     4021 ttactcagag ccggagggat aacacagtgg atgaaggcag ctcacctagc tgaggcttac
     4081 aatttaccag ttgttacaca tttggccaca gaggtcttga tgcactgctt ggctgcttgt
     4141 ccaaacggtt tgtacgtgga acacatgcca tggacctttg ccatgttcaa tgaggaaccc
     4201 gaagttgtcg gtggggaaat tgttttgcct caaagtccag gattagggtt gtccttcaac
     4261 gacgacaatt taaaaaaata tgcggtttct taatttttat agatacccac caataattgc
     4321 atacctactt agtcctaggt gatatggtaa actgaggggt ggatttatag ctcgattttc
     4381 actttcttag aatctctcaa ggagttttga attggcaacg cagacaccga aaaattacag
     4441 cgccattggg aaaagacctg tccgacacga tggagcagat aaagttaccg gaaaagctca
     4501 atacggagcg gatttgaact tgcccagaat gttatttggc aaggtcctac ggagcccaca
     4561 cgcacatgcc cgaattaagt caatcgatct gtctgcagca cttgctcacc cagaggtcaa
     4621 ggctatcgtc acctccagtg atttatcgcc tttgccggat aaacctgcag aggtgggtga
     4681 agacctctat gcgaatatga agtatgtgag agacaggatt ctcgcttctg ataaagtttt
     4741 atttaaaggt caccctgtgg cagccattgc ggctaatggg cttcatgaag cagaagaact
     4801 tttatcattg atcaaggtgg aatatgaagt tctggaatcg gtgaccgacg tcgaatctgc
     4861 gatgtctgaa aatgccccaa tactccatgg cactattaga tttgcctcga tgtcagggga
     4921 cgaagttcat ggctccaaca ttgctagtca tgatcaatac atactcgggg atataaatga
     4981 aggctttaaa aaggccgacc taactattga aagagaatat cggaccaaaa cagtgcatca
     5041 aggctatatt gaaccacaaa acggtacagc ttcctggagg tcggatggcc acgttaccat
     5101 ttggtgcagc agccaaggac atttcgggat cagagatcaa gtttctacta ttttgggtct
     5161 gcccgtttct aaagtaacta tagtccccat ggaaattggc ggtggttttg gtgggaagct
     5221 tccttgctat ttagaaccct tagccgccct tctatccaaa aaaactggta ccccagtcaa
     5281 gatgttcatg acacgatctg aagtgattga ggcctcaggg ccaacctctg gaagttttgt
     5341 tagggtgaaa ataggtgtta cgaatgatgg gaaaattact gccgccaaag gctatcttgc
     5401 tttcgaggct ggtgcatttc ccggctcccc cataggagga gctaccgcat gtatgctttc
     5461 cccttatgac atagaaaatc tacagctgga tggatatgat gtggtcgaca ataaacctaa
     5521 aactacggca taccgaggcc ccggggctcc cataggtgcc ttagcggtgg aaactgttat
     5581 agacgagatc gcggagaagc tgaaaatcga cccggtcgat cttcgactgt tgaatgctgc
     5641 caaggaaggt acacgaagat cagacggagt tgtcaacggc aagattggaa tgattgagct
     5701 ttcggagatt gtaaaaaatc acgaccatta ccaaacacct ttggaagaag ttgaaggtaa
     5761 gttaagaggc agaggaatag cgttcggata ctggagaaat aacactggac cggccgcatg
     5821 cgttgccact gttacaccgg atggaacagt caatctatct gaaggttctg tggatgttgg
     5881 gggctctaga actgcaatag cacaacaact tgctgaagtc ctctccatac cagttgaaga
     5941 cgttaaccca caagttgccg acactgattc cataggatac acatcagtaa caggtggcag
     6001 tggggttgct tttaaatctg gctgggctgc atttgaagcg gcacaagaag taaaaaaaga
     6061 aatgttaaag agagcaagtt tggtatggga cgccaaggaa gatgaagtcg aatatgccga
     6121 tggaattgcc caacacaaat ccgaccctga acttaaactg acttttaagg aaattgcagc
     6181 agcttcgaat ggaacaggtg gaccgattgt tggtagtgct ggagtcaacc ctagaggcgt
     6241 cggaggatca ttcgcagcta tgcttgtaga tgtagaagtc gatgaggaaa ctggcaagac
     6301 gacaatcctt cggtgcaccc ctttcactga cgtagggaag gcaattcatc cagcttacgt
     6361 ggaaggtcaa atccaaggag gcacggcgca aggtatcggt tgggctttga acgaggaata
     6421 ctttatgagt gacgatggta aagtcatgaa tgctagcctt ttggattatc gaatgcccac
     6481 aagtttagac ctgcctatga tagatcctgt ggtggtagag gtatttaatc cagggcaccc
     6541 tttcggtgtg agaggtgtag gagaatctcc tctagtacca ccactcgctg caatggccaa
     6601 tgccttatat catgctaccg gagtgagaat gcgagaatta ccaatgtctc ctacagctgt
     6661 aaaaaaggct atcgataacg ccaaaattta gtcccaggct taaattttag tatcatttaa
     6721 ggtacgtacg aaaagaaatt aacgaggttt aataatgcca aaacaattcc aatttaaaga
     6781 caatgcccga actcaattga tggaaggtat cgatcttatg gcccgcactg tgggaataac
     6841 tttaggacct agtggtcgga atgtaatctt agaaaaagag ttcggccccc cccaggtatg
     6901 tagtgatgga gtaaccatag ccaaagagat tgagttgctt gaaccatttc acaatatggg
     6961 tgcgcagcta cttaaggagg cggcctcaaa gacaaatgat gatgtgggag acggtaccac
     7021 cacatccaca atcttagccc aagctatatt aaaaaacggt tttaagaata tagccgccgg
     7081 tgctgaccca atggccctga aaagaggtat tgatcaagca gtggatgtga taaccaaaga
     7141 attgataaat atctcaaaag atgtcaccgg agacgaacag atttcgcaag tagcggtgct
     7201 ttccgcacat gatgagaaaa tgggccaatt gataggccag gtgatgagta aggttggaaa
     7261 agatggcgtc gtaactgtcg aagaatcgcg aggccaggac tatgaggtcg aatatgtaga
     7321 aggcatggaa atagataggg ggtatttatc cccttatttt gtgactgacc aagatagaat
     7381 ggttacagag cttaatgagc cctacatcct cattacttcg gaaaaaatta cgagtctaga
     7441 agaattactc ccatttcttg agaagtttag cgctgtcggc agagaactag tcctgatagc
     7501 tgaagatata gaagagcagg ccctagccac acttgttgta aacaaaatga aagggacact
     7561 aaattgcctt ggggtaaaag ctcctgcttt cggagatagg agaaaagcta tcctggaaga
     7621 tatgtccatt cttttcggtg gaacagtcat aagtagtgaa acaggtagaa ctcttgattc
     7681 catcgagatt tcagatttag gaagatgcag aagggttata tccaccaagg attccactac
     7741 tttcgtagag ggaagtggag aggcaagcct tatcgatgca agggtgagta atataaaatc
     7801 tcaggctcaa gagaccaaat ctgattacga ccgtgaaaag ctggaagagc gtgcggcaaa
     7861 actatcgggt ggtgtgtcga taataaaggt cggagccgca acggaagttg aattgaagga
     7921 aaaaaagcag agagtggaag atgcattatc tgctacccgg gcagctatgg aagaaggaat
     7981 cctcccagga ggtggtacct cacttatcag aatagctgct gatttacgtg aaaattactc
     8041 agatagttct gatgaggcaa ctggggccag gattgttttg gattctgtag atgcacctgt
     8101 tgtacttttg atacaaaatg ctgggtttag tggtcaggta gtactggatg cgattaaaaa
     8161 aggaagtggt gactacgggt ttgatgctga aaatgatagg tatggcagta tgtacgaatt
     8221 cggtgtgatc gatccagtga aagtcacgag gtcggccctt gaaaatgcag caagcatagc
     8281 tggtatggtc ttaaccacgg aatctctcat tacagagttg gatcctccta aattaccagc
     8341 accatttgac gactaaattt tcattctcaa cagggcggcc tcaaaaaacc gccccaaagg
     8401 gaaagaccta tgtcaaccga taaagaaaat gaaaatccta tagacccgga cgttactatt
     8461 tcagtggaaa tagtgagtaa gagctttgga cctcacaaag ctgtatccaa cctatctttt
     8521 tcaatcaaaa gaggagagat agttggtttt ctaggcccaa acggggccgg taaaacgact
     8581 accatgagat tgctgacttc ttattacact ccagacaccg gtaaaatcct tataaacgga
     8641 atagacaatt cagagaacga cttagaaacc aggaaaagca ttggatacct agcggaaaac
     8701 aatcctttgt atgaagattt attggtcagt gaatatcttg attttatagc tgatctcagg
     8761 ggactttcag gcaaggaaag aacccgtaac ttggaacaaa ccgttgaaga ggccagctta
     8821 caagaagttt tctatcggcc cataagcgaa ttatccaagg ggtatcatca aagggtaggc
     8881 ttggctggcg ctataattca ccggccttcc atcctgatac tggatgaacc taccgaaggt
     8941 ttagacccca atcaacgttt atcgatgagg gatttaatca aatctttggg tcaagatcga
     9001 acagttttag tgacaactca cgttatgcaa gaagtagaaa atacctgtga gcgagtgtta
     9061 cttataaacc gaggccaact cacagctgac agtccggtcg atgaaatttt tcaactcaca
     9121 cctgggttaa gaaaaatata tgtggaggcg gaaggtaatc aaattgaatc aggtttatct
     9181 tcactagatt cggtgaaatc tgtcgaacga ttggatccga ttgattctag aaaaagatac
     9241 atcgtcaatc tatcaggttc cgaagatcca agaccagcta tatttaaaat tgctactaac
     9301 aatggatgga tactgtggga attgcatgaa gaacgcatgc gtctagaaga cgtatttcac
     9361 accctcacat cagaaaatga ggctgaaagg tcgctactag gatcatgaaa tctccatttt
     9421 tagctttaat taagaaagac ctcaaagggt attttgacca acccaccggt tacatattga
     9481 tcgtcgtctt tgtgagtatg ctggcttggt catttttcca gtcggccctt ttaatcggag
     9541 aggcttccct aagaccttta ttcacagttg acttcgccat agacaagcca tccttaccgt
     9601 ggctgttagc cctatttata ccggctgcca cgatgagatt actgtcagaa gaacaacggg
     9661 atggtacctt agaaacccta ctaactcagc ctattagagg ttggattgtg ctgttagcga
     9721 agttcacatc aggactatta tttgtgtcga tagctatcgt agctaccata ggca
//
LOCUS       DASCNS010000207        15967 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001585152, whole genome shotgun sequence.
ACCESSION   DASCNS010000207 DASCNS010000000
VERSION     DASCNS010000207.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 15967)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15967)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15967
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001585152"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="QF838_07555"
     CDS             complement(<1..957)
                     /locus_tag="QF838_07555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60571.1"
                     /translation="MARLDTWLERLGNRWFFYGWLIVFSSFTSSMINAGTGSYALGFF
                     IIPMGEEIGISRTQFSVIPIFKLATIPILPLLGLLVDRRHGGRIIVSVGSLVGGTALA
                     FTSQIDKVWQFYMLYGIIYGFGTAAMGSQLVGPSLMAKWFVRMRGRAMAIGTMGISAG
                     GVVVAPVAGLAISTLGWRAAWVVLGMMAVVAIVPVSSLFLRRSPEDIGLLPDGNASEL
                     NAEVAADNEVNSWTLNQSIRDTQFWILLLIQCLGLSGLVVVLFHEVAYIQDKGLNIQS
                     ATLIATSLATSAMISKLPFGYLAEKMDIRRVLALCLIPAGLST"
     gene            complement(993..2330)
                     /locus_tag="QF838_07560"
     CDS             complement(993..2330)
                     /locus_tag="QF838_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HJO60572.1"
                     /translation="MGKSLEYSTIEELSELLSSSKISTKELVLHLLDRSKKLDPNLNV
                     WVTLNPNLETENYSQESTNKLAGIPIGIKDIYCTRDMRTTCCSPIYENYEPGYDARTV
                     KMLRDSQAVIMGKTVTTQFACGDPPPTKNPWDFSRTPGGSSSGSAVGVASGMFPAALG
                     SQTAGSVLRPASYNGIVGFKPTYGLVSRQGVFPVALSLDTMGWFTRSVKDAAILLQYL
                     SGYDAEDKDSVEVDSKPYISIPKPISRPPHIGVVENFYYENSDHSTMKNFALLLERLS
                     KAGAIIETADTQLDFNSILDSHRVIMNCEAAHTHKDNFKKRPDDFAPRVKEIIEHGLN
                     TPTHIYIQSKHFQQNYIKQVSKLSKKYDALIMPTSLSGAPTSETTGEPIFQAPWTMSG
                     VPAISLPYALDNDGMPLGVQIISDHFDESNLLSTSMWIESVVNFTHKPPLFND"
     gene            complement(2346..3254)
                     /locus_tag="QF838_07565"
     CDS             complement(2346..3254)
                     /locus_tag="QF838_07565"
                     /inference="COORDINATES: protein motif:HMM:NF028222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO60573.1"
                     /translation="MEPGKALVKTNLASICGSDLHITYMGWNAHEFPLPHGYPGHEGI
                     GQIVDPGDTDFAEGELVLTVPNIWNSKCFAGYQLIDPNFLLRVPTGVPEAHLLMAQQL
                     GTVVFGTRKLPTLVGKTIAILGQGSVGLFHDFMLRKLGAHKIIAIEPVAERLQAGRNM
                     GIDEAIDVTGDKATEAVMDITSGEGADLVIEAVGSVDTLNQSLKLVKPFGKLAVFGLP
                     PTMEKVPFDWDTFFRKCIDMHSVFGAQDEPGLPAFQLAVDYIENKEIDMAPFVTHQFP
                     ISEIQNAFNLADSREDGALKVSLTFE"
     gene            3509..3697
                     /locus_tag="QF838_07570"
     CDS             3509..3697
                     /locus_tag="QF838_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60574.1"
                     /translation="MESNLKNELKELNEEIIYYPGPIAGCDVQFDWLLEERIRLTNQI
                     KKMTDISHREPADGIAQG"
     gene            3701..4102
                     /locus_tag="QF838_07575"
     CDS             3701..4102
                     /locus_tag="QF838_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HJO60575.1"
                     /translation="MKTIAGYWIGVGSILAFLSVLVGALGAHWLEPVMDMDAKDIYET
                     SVRFQMFHSVALILIGIILNFKVGFNRFFFYIPWLIFVGTVIFCGSLYTISLSSFNWF
                     GAIAPFGALLFIFGWLLLAFSVIDLSRQKPK"
     gene            complement(4157..4978)
                     /locus_tag="QF838_07580"
     CDS             complement(4157..4978)
                     /locus_tag="QF838_07580"
                     /inference="COORDINATES: protein motif:HMM:NF040043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HJO60576.1"
                     /translation="MNDEIESLLRGSIDLDVHAAPDIPKRRMDALETTRAAYEAGMGG
                     FVLKSDNYLTYPLTYALSQMYPGLEIYGSISLNSPVGGINPEAVETAARLGTKVVWMP
                     TISAAFHLPKSNSGNGLNILDKSEKLTDDIFEIIDIVQGNDMVLASGYISPLETIKLF
                     GAAKDHGVRKMIVTHPEELLEDQLKNLTTLGAYMEYSFLSCMPLTAKISPMGFSQQIL
                     KAGIERSVVTTAFGQPVNPPPSEGMRMAIAALLDSGMSPKNISKLVKDNPSDLLS"
     gene            complement(4998..5639)
                     /locus_tag="QF838_07585"
     CDS             complement(4998..5639)
                     /locus_tag="QF838_07585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HJO60577.1"
                     /translation="MTPDLVIFDCDGVLVDSEVISNSVLSESLEAIGLNITPEVCMDL
                     FLGKSWDYCFQVIKKRLGTEPPRTLYETYMNELFAQFEKTLKPIEDIQFALDNIPYPK
                     CVASSGPHLKIHKTLSIAGLMERFGKHLYSAEDVKFGKPAPDLFLHAAMKMKATPSKT
                     LVIEDSTAGIEAAYSAGMTPLAFCSKKPNRAIIQSETIVFRSMRELPDLINRL"
     gene            5802..6278
                     /locus_tag="QF838_07590"
     CDS             5802..6278
                     /locus_tag="QF838_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HJO60578.1"
                     /translation="MPHRDSRNQVPANQRVIRLGVSVNPRTPRPGRGRPRTRSSESVY
                     ASPDGLYVISVAARLLEMHPQTLRKYERSGLVRPSRTVGMLRLYSEEDIVRLRLIKHL
                     VGDLGLNLAGVELSLGMFNQMLKMKSGLDQAENGELKKYLENCLNEMFKMLKTRPS"
     gene            6309..6899
                     /locus_tag="QF838_07595"
     CDS             6309..6899
                     /locus_tag="QF838_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HJO60579.1"
                     /translation="MEEKEISGNPTPGPNDQELNNLESEEDSTVVPETSIDLSEEIDH
                     YKSIAQRAQADLVNYRNRASQEIEEAKRAVQFGVISRFISIVDDLERAVENAPEGAQT
                     EWTEGVELVLRNLEKALELEGVSQIESLGKSFDPREHDALLYEENSDTEDGLILNVIQ
                     QGYRLNERIIRPARVIVSKFTEQENQEDELSKQEEK"
     gene            6899..8794
                     /gene="dnaK"
                     /locus_tag="QF838_07600"
     CDS             6899..8794
                     /gene="dnaK"
                     /locus_tag="QF838_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HJO60580.1"
                     /translation="MPKILGIDLGTTNSCMSIIEGGEPRVIENAEGNRTTPSVVGINP
                     RSNERYVGTTAKRQAVTNPDNTVFSAKRFMGMKYGDDSVSRNKELVPYSVVEHTNGDA
                     HVSMGDKNYAPPEVAAMVLQKLKQDAESKLGEEITQAVITVPAYFDDSQRNATRDAGK
                     IAGLEVMRIINEPTAAALAYGLDNESDQTIAVYDLGGGTFDITVLQMGDGVFEVKATN
                     GDTHLGGDDFDQLIIDWICDKFKQDQGIDLKSDRMALQRLRESAERAKIELSTLMQTE
                     INLPFITADTSGPKHLVETMERSTLEQLVNDLVQQTIEPCKKAMSDAEVSSSEIDEII
                     LVGGMTRMPVVQKAVEDFFGKQVHQGVNPDEVVAIGAAVQAGVLQGDVQDILLLDVTP
                     LTLGIETLGGVTTPLIQRNTTIPTSKSETFTTASDNQPSVEVHVLQGERSMANENKTI
                     GRFNLDGILPAPRGVPQVEVTFDIDANGILNVSATDKGTGKEQRITITASSGLSQDEI
                     DQMIDEAEQNAEEDRQKREMVEIRNTAQSAVFAAETLVKENEEHIDASLKDEVEQKIS
                     DLKTALEGEDNPAIESALQQLQESLQKIGQEVYSKTGEAPGDQPEEESSSADDPENTV
                     EGEFREV"
     gene            complement(8840..9826)
                     /locus_tag="QF838_07605"
     CDS             complement(8840..9826)
                     /locus_tag="QF838_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HJO60581.1"
                     /translation="MAATKAHSKLVQQMTEAGSKFLESLSPDQKSKACFEYLDGERIF
                     WYYPPMNRHGLALRDMDPNQRDLAFKLMSTGLTDRSYEQARQIIALENVLGPLEKESG
                     VKSFKRDPELYYFTIFGTPGGDDPWAWRAEGHHVSLHFSVWKGEIIAMTPFFFGANPA
                     EVRKGPQNGLRILSHREDIAVDLMSSFDEGQQKKAIIYGSAPNDIITYNSSKVSLPAE
                     EGLSISKMTGTQREIIESLISEYINQVPEELAQKKFDELERYGLGNVHFAWGGATDTS
                     GEHYYRIHGGNFVVEFDNRQNGANHIHSVWRDVDNDFAEDVMRQHLLAYHVL"
     gene            complement(9909..10859)
                     /locus_tag="QF838_07610"
     CDS             complement(9909..10859)
                     /locus_tag="QF838_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013595.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO60582.1"
                     /translation="MGSQVTIGDGDFKYNVVENWEQMPTDYSWSETAGVATDKKDNVY
                     VFNRGEHPMMVFDKDGKFLKSWGEGVFTRAHGVSRGPNDTLYCTDDGDHTVRQCTLDG
                     KVLMTIGNPGNPSALFSGEPFNRCTHTATDPETGDIFVTDGYGNSRIHKYSPDGNLLQ
                     SWGGPGTGEGEFNIIHNIATDKNGYIYICDRENHRVQVFDKRGNFEAAWSNIHRPCAI
                     YIDEQQRIYVAELGYGNNVSKNVPNIGPRVSVLDQSGKVLERIGDMGYGFATGQFAAP
                     HGLCLDSNLSIYVAEVARTNMSHYTTPPDVLRSFQKLVKV"
     gene            11000..11443
                     /locus_tag="QF838_07615"
     CDS             11000..11443
                     /locus_tag="QF838_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HJO60583.1"
                     /translation="MAGSIVSLRLCLGSREPMKEIAQAEFLTGQGMQGDRHMRSDGLR
                     SKRQVLVMDIETLNHFDLKPGQVRENVTLKGLDLSTISAGDRVSLGADVVLEITGDCE
                     PCSRMDEIRPGLKEEIDGKRGLLAFVEKGGIVSVGAEVGVNTFQL"
     gene            complement(11472..12227)
                     /locus_tag="QF838_07620"
     CDS             complement(11472..12227)
                     /locus_tag="QF838_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60584.1"
                     /translation="MNMNNLEGKVAIVTGGATGIGYGIAKRLANDGANLVMVDLDANM
                     MKQSASEITARTQEVEISIGDVSEPKTAQEAVSKAINKWGKIDILVNNAGIGGVNGNI
                     WELDIEEMDRVYRTNLRGVFSFCREVIPHMLEKDYGRIVNIASIAGKEGNPRMVPYSS
                     TKAAVIGLTKSIGKELAGTGVLANCVTPAVVQTRILEEFTPEQVQYMVDRIPVGRTGE
                     ISEIAAMVAWLSSSECSFSTGAVFDISGGRATY"
     gene            complement(12249..12473)
                     /locus_tag="QF838_07625"
     CDS             complement(12249..12473)
                     /locus_tag="QF838_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60585.1"
                     /translation="MIGLPENDVYEEETNIIYMKEIPLIFPFALLIILVALMFSTAYG
                     LADSTVKKKGPSPSQYEWEEKAALWVCPFH"
     gene            complement(12451..13887)
                     /locus_tag="QF838_07630"
     CDS             complement(12451..13887)
                     /locus_tag="QF838_07630"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HJO60586.1"
                     /translation="MKLKPVILAGGSGKRLWPMSRNQYPKPFLTNNSQTSLIQNTVKR
                     SQNLGLALPVIVCNEQNRFLLTNHLNSISAPVEKILLEPFPKNTAPALTLAALYLLRN
                     FGDHILFSMPSDHFIEEDENFFEVVDTASKLAEDGGIVTFGIDVQTPDVNFGYIQKGD
                     STKNNYYELKRFHEKPDSKTARRMHESKEFFWNSGMFLVKASSWIHYLKIHSPRILEA
                     CERSIQNGVEDGIFFRPESKSLNDCPSKSIDHAVMENLEFPGNIFKNWVIPLKSVWSD
                     LGSWGSFMSSQNPDKSNNLLHGEVITDSVQNSILISDHRTIAASHVDNLIVVETPDAI
                     LVADRNQEHRIKDLVDKIPAENTAIFESHKKEFRPWGHYEILDSGKEFQVKRLTVLPK
                     HALSLQIHHHRTEHWVIVSGVATITRGSETMTLKKNQSTYIPRGVQHRLENDEGENLE
                     IIEVQSGSYFGEDDIVRLEDRYDRTSRK"
     gene            14087..14941
                     /locus_tag="QF838_07635"
     CDS             14087..14941
                     /locus_tag="QF838_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60587.1"
                     /translation="MGTLDGKTVVVTGASRGIGAEIARIFAKEGGKVVCAARTLKEGD
                     HPLEGSLEKTVSDINDAGGEAKPVIANISLPEDCERLFAETHELYGKVDVLVNNAALT
                     YFIPVRDYPVNRWMRSWAVNFHAPFILSQLALEDMISMGSGSIVNISSGAAIGPGRGP
                     YSNVPPNSGGTCYGAEKAALERFTQGLAQEVYGDGISVTCVSPSQVVPTPGTVFHNLV
                     TGMDDPRGEHPELMAKAALLLATEPMDKVTGRVTYSQQILKEFGWIDEAKGTGVDQDR
                     VGSGYSQV"
     gene            15126..>15967
                     /locus_tag="QF838_07640"
     CDS             15126..>15967
                     /locus_tag="QF838_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024573.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJO60588.1"
                     /translation="MKLTQARVINYRSIDDSSWVRVDDVTALVGKNESGKTAFLQAIR
                     KINSISGEEDTFSIRDYPRKGYIKYKKIHDQNPCEVAQAEFELNAEEISEIEANFGDG
                     ILASNKVIVTKNYKNERNWKISLAESVSQPPNVTTFTQPSTPAPTSFQSAVQAVDEVS
                     NVSERISKQFLEKWLPKFVYFDNYSLMRGKISINELRQRSENGGPFDDADRTFLSLLT
                     LSGVSLEDLEKDLGYEDIKVELESASITITDEIFEYWQQNRQLKVEFDVSQADPRDAA
                     PLNQG"
BASE COUNT         4553 a         3202 c         3472 g         4740 t
ORIGIN      
        1 ggttgataat cctgcaggta tcaaacaaag tgcaagaact cttcttatat ccattttttc
       61 tgctaggtag ccaaaaggga gtttgctaat catggctgag gtggctaatg aggtcgctat
      121 caaggttgct gactgtatat ttagtccttt gtcttgaata taggcgactt cgtgaaatag
      181 tacgaccacc aaacctgaaa ggcctagaca ctgaattagc aaaagtatcc agaattgggt
      241 atcccgtatg gattggttca gggtccagga atttacttca ttatctgcgg caacttctgc
      301 attcaactcc gatgcatttc catcaggcag tagcccaata tcttcggggc ttctcctaag
      361 aaatagtgat gatacgggga cgatagcaac taccgccatc attcccaata ctacccaagc
      421 tgctctccat ccaagggtgg agatggccaa acctgcaaca ggcgcgacta caacaccacc
      481 ggccgatatg cccatggtgc caatggccat cgctcttcct ctcattctca caaaccattt
      541 ggccatcaat gacggaccaa ccaattggct acccatggca gcagtgccaa agccataaat
      601 aattccgtat aacatgtaga actgccacac tttgtctatt tgagaggtaa aagccaaggc
      661 agtacccccg accaaactgc caacggaaac tattattctt cctccatgtc ttctatccac
      721 caataggcct aatagcggga gtattggaat tgtggcgagt ttaaatatag gaattactga
      781 aaactgggtt ctggatattc ctatctcctc acccatcggt atgatgaaaa atcctaaagc
      841 atagctacca gtgccggcat ttatcatgct tgatgtgaaa gaagaaaata ctataagcca
      901 gccatagaag aaccaccggt ttcctagtct ttccagccat gtatccaatc tagccatact
      961 attggaaaaa ccctaagggt ctgacaggat ttttaatcat taaatagagg tggcttgtgg
     1021 gtgaaattca ccacggattc aatccacatt gaagtcgata gtaaattcga ttcgtcaaag
     1081 tggtccgata ttatttgtac tcccagtggc ataccgtcat tgtccaacgc gtatggcaaa
     1141 gagattgctg gcaccccgga cattgtccac ggagcctgaa agataggttc ccccgtagtc
     1201 tctgaggttg gagcacctga cagacttgtt ggcattatca aagcatcgta tttctttgac
     1261 aattttgata cttgcttaat gtaattctgt tgaaaatgct ttgattgtat atatatatga
     1321 gttggtgtat tcaaaccatg ttcgattatt tcttttactc tgggagcaaa gtcatcaggc
     1381 ctttttttaa aattatcttt atgggtatgt gctgcctcgc aattcatgat gactctgtga
     1441 gagtctaaga tgctattaaa atcaagttgt gtatccgcag tctctattat cgcccctgcc
     1501 tttgaaagtc tctccaataa aagagcaaaa tttttcatag tgctgtgatc tgaattttca
     1561 tagtagaagt tttctacaac gccgatatga ggtggtcggg agatcggttt aggaatcgaa
     1621 atatatggtt tgctatccac ttccactgaa tccttatctt cggcatcata accagataaa
     1681 tactgcaaca ggattgcagc atcttttacc gagcgggtaa accatcccat tgtgtcaaga
     1741 gataatgcta ctgggaaaac tccttgccta ctgacgagac cgtatgtcgg tttgaatcca
     1801 acgataccgt tatatgacgc aggccttaaa acggaacctg cagtttgaga ccctagggca
     1861 gccgggaaca tccccgaagc tactccgact gctgaaccac tactggatcc tcctggtgtt
     1921 cttgaaaaat cccagggatt tttggtaggg ggaggatctc cacaagcaaa ttgagtagtt
     1981 acggttttac ccatgatcac agcctgggaa tctcttagca tttttaccgt ccgagcgtca
     2041 tacccaggct cataattttc atatattgga gaacaacagg ttgttctcat gtcgcgggtg
     2101 caataaatgt cctttatgcc tataggtata ccggctaatt tattagtact ctcctggcta
     2161 tagttttcgg tttccaaatt tgggttgagc gttacccaca cattcaaatt tgggtcaagt
     2221 tttttagaac gatccagtaa atgaagtacc aattcttttg ttgaaatttt tgaagaactt
     2281 agtagttcag acaattcctc tatcgtagaa tattcaagtg atttccccat ttaggctcct
     2341 cattttcact caaaggtaag ggaaactttt aaggctccat cctcacgaga gtcggctagg
     2401 ttaaaagcgt tctgtatttc agatattgga aattggtgag taacgaaggg tgccatatca
     2461 atttctttat tttctatgta gtccacagct agttggaagg ccggcaaacc aggctcgtct
     2521 tgggctccaa acactgaatg catgtctata catttacgga aaaaggtatc ccaatcaaaa
     2581 ggaacttttt ccatggttgg aggcaaccca aacactgcca attttccgaa aggcttaact
     2641 aatttcaaag attgattcaa agtgtcaaca ctgcctacag cttctatgac caggtccgca
     2701 ccttcaccgg atgtaatgtc catcacggcc tctgtagctt tgtcacctgt cacatcaatt
     2761 gcttcgtcaa ttcccatatt gcggccagct tgcaacctct ctgctactgg ttcgatcgct
     2821 atgattttat gagctccgag tttccgtaac ataaaatcgt ggaataaacc aacagaacct
     2881 tgtcctaaga tcgctatagt ttttccgact aatgtcggta attttcttgt tccaaaaacc
     2941 actgtcccaa gctgttgtgc cattaacagg tgcgcctctg gaacacctgt tggtacccgt
     3001 agaaggaaat taggatcaat taattggtag cctgcgaagc acttggagtt ccaaatattt
     3061 ggcacggtga ggaccaattc cccttcggca aaatctgtat caccagggtc tactatttgt
     3121 ccaatacctt cgtgtccggg gtaaccgtga gggagaggga attcatgtgc attccaaccc
     3181 atataagtta tgtgcaagtc actaccacat attgatgcca gattcgtttt tactaatgct
     3241 ttgccaggtt ccattttggg atatctatag catcacattg ccattctctc aacccagttg
     3301 atatcgctgc gggcatctcg ctcatcagtg tctccgtgta taaaacatgt ctttttgtag
     3361 atgtctggaa ttgtaccctc gtgtatcctc ataatacaaa attaatttgc ccgcataggc
     3421 tttttgggca aaaaggtgta aaggttaagt ttctccctgc tatcatgagg cctgagcaat
     3481 taacagaatg gtcggctaag gtaataaatt ggaatcaaac ctcaaaaatg aattgaaaga
     3541 attaaatgaa gaaataatat attatcccgg cccaattgct ggatgtgatg ttcagtttga
     3601 ctggttattg gaggaacgga tcagattaac caatcaaatc aaaaaaatga cagatatttc
     3661 ccatagagaa cctgcagatg gcattgccca aggttgatat ttgaaaacta ttgctggcta
     3721 ttggataggt gtaggttcaa tattggcttt cttgtccgtt ttggttggag ccttgggagc
     3781 ccattggctt gagcctgtca tggatatgga tgctaaagac atctacgaaa cctccgtcag
     3841 gttccagatg tttcactcag tggctttgat tcttatcgga ataatattga atttcaaagt
     3901 tggatttaac aggttctttt tttatatacc atggttgatt tttgtgggga ctgtcatatt
     3961 ttgcggtagc ttgtacacga taagtctgag ctcttttaat tggttcgggg ctatagcccc
     4021 tttcggggcc ttattattta tatttggttg gttgctgcta gctttttcag taatcgattt
     4081 atcaagacaa aaaccgaagt aaagatgatg ggaaaacaag ggccagattt tattattagg
     4141 agcggagatt ttttaactag ctcaataaat cactagggtt atctttcact aatttagaaa
     4201 tgttcttcgg tgacattcct gaatccagca atgcagctat agccatccgc attccttcag
     4261 atggtggagg gtttactggc tgtccaaaag cagttgttac tacggatctt tcaatgccag
     4321 ctttaagtat ctgctgtgaa aaacccatag ggcttatttt tgcggttaaa ggcatacatg
     4381 aaagaaagct gtattccatg tatgctccca gtgtggtcaa atttttcaat tgatcttcaa
     4441 ggagttcctc cggatgagtt acgatcattt ttcttacgcc atgatctttg gcggccccaa
     4501 ataattttat cgtttccaat ggtgaaatgt atcctgacgc caagaccata tcatttcctt
     4561 gaacgatatc aataatttca aaaatatcat cagtaagctt ctctgatttg tctaatatat
     4621 ttaagccatt cccagagtta ctttttggta ggtgaaacgc agctgaaata gtaggcatcc
     4681 agacaacttt tgtacctaac cgggccgctg tttccactgc ctctggatta attccgccta
     4741 caggtgaatt taatgaaatc gaaccgtata tttctagccc tggatacatt tgagataagg
     4801 cgtaggtcaa agggtatgtg aggtaattgt ctgattttaa aacgaatcca cccatccccg
     4861 cttcataggc tgccctcgtc gtttctaagg catccattcg ccttttgggg atgtcagggg
     4921 cggcgtgtac gtcaagatca atagatcctc tcaacagcga ttcaatttca tcgttcacaa
     4981 cttcctccgg ttgctgatta caatctattt atcagatcgg gtaattctct catgctacgg
     5041 aaaactatgg tttctgattg tataattgct cgattaggtt tttttgagca aaaagctaga
     5101 ggagtcattc cggcgctata ggccgcttct atgccagccg ttgaatcttc tatcaccaac
     5161 gtctttgacg gggtagcctt cattttcatt gctgcgtgta gaaaaaggtc tggggctggt
     5221 tttccgaatt tcacgtcctc cgcgctgtat aggtgctttc caaatctctc cattaaaccg
     5281 gcgatagata aggttttatg tatctttagg tgtggtccgc tggatgccac acatttcggg
     5341 taaggtatgt tgtccaaggc aaattgtata tcttcgatcg gcttcaaggt tttttcaaac
     5401 tgggcaaaca gttcattcat atatgtctca tatagagtac gaggaggctc tgttcccaaa
     5461 cgttttttaa tcacctgaaa gcaataatcc caagatttgc ccaggaatag atccatgcat
     5521 acctctggtg tgatatttag tccaatagct tctaatgact cagaaagcac ggagttagat
     5581 ataacttcac tatcaacaag tacaccatca cagtcaaaaa taactaaatc aggtgtcata
     5641 actgtttttc atcggtgtgc tcgctggcat tagacaattg gtttgagtat gggtaataat
     5701 caatataacg taagttgaca cgaaacgaat caacttataa catttattca aataaatgtc
     5761 tgggttttag tatacatcta atcaaaatgt agaaataatt tatgccacat agagattcta
     5821 ggaaccaagt acctgcaaac cagcgtgtga ttcggctggg agtttcggtt aacccgagga
     5881 ctcctagacc agggcgtggt agaccccgaa cgagaagctc cgaaagtgtc tacgcttcac
     5941 cggatggact ctatgtcatt agtgtggctg cacggttgtt agaaatgcat ccgcaaacct
     6001 tgcgtaaata tgaaaggtcc ggattggtaa ggccgtcaag aactgttggg atgctcagat
     6061 tatattcgga ggaagatatt gtcaggctaa gacttattaa acatttagta ggtgacttag
     6121 gtttgaatct agcaggtgta gagctttcgc ttggtatgtt taaccagatg ttgaaaatga
     6181 aatcaggatt ggatcaagct gaaaatggtg aattgaaaaa atacttggaa aattgtctga
     6241 atgaaatgtt caagatgcta aagactagac cttcttaagt ttatattttt atttgatgga
     6301 gaaaagattt ggaagaaaaa gaaatatccg gaaatccaac cccgggacca aacgaccaag
     6361 agttgaataa tcttgaaagt gaagaagatt cgacagttgt accagaaaca tcaattgatt
     6421 taagcgaaga aatagaccat tacaaatcaa ttgcccagcg agcacaagcc gatttagtta
     6481 attatagaaa tcgggctagc caggaaatag aagaggccaa aagggcagtc cagtttggtg
     6541 ttatttcaag attcatttct atagttgatg atttagaaag ggccgttgaa aatgcccctg
     6601 agggggccca aactgaatgg acagagggag ttgaattggt gctaaggaat ttggaaaaag
     6661 cactggaatt agaaggtgtg agccagattg aatctctagg taaatctttt gaccctagag
     6721 aacatgatgc ccttttatat gaagaaaatt ccgatactga agatggattg atactcaatg
     6781 tgatacagca aggatatcga ctaaatgaaa ggatcattag gcctgcaagg gtaattgttt
     6841 caaaatttac cgaacaagaa aaccaagaag acgaattaag taaacaggag gagaagtaat
     6901 gccgaaaatt ctcggtattg atttaggtac taccaattca tgtatgtcaa ttatagaagg
     6961 tggtgaacct cgagtgattg aaaatgctga gggtaatagg acaacacctt cagtcgtagg
     7021 tattaatcct agatctaatg aaagatatgt cggaacaaca gctaagcgcc aggcggtcac
     7081 aaatcccgat aacacggttt tttccgctaa acgctttatg ggaatgaaat acggggatga
     7141 ttctgtatct aggaataagg aattggttcc ctactcggtg gttgagcaca caaatggtga
     7201 tgcccatgtt tctatgggag acaagaacta cgctccacca gaagtggcgg ctatggttct
     7261 tcagaagttg aaacaggatg cagaatccaa attgggggaa gaaattacac aagctgtaat
     7321 tactgtgccg gcgtatttcg atgatagcca gagaaatgct acgagggatg ctggaaaaat
     7381 cgcaggccta gaggtaatga gaatcattaa cgaaccgaca gcagcagcac tggcatacgg
     7441 tctagataat gaaagtgacc agacgatagc tgtatatgac cttggaggag gcacctttga
     7501 tattacggtt cttcaaatgg gggacggggt ttttgaggtt aaagcaacca atggagacac
     7561 tcatttggga ggggatgatt tcgatcaact tattattgat tggatctgcg ataagtttaa
     7621 acaagaccaa gggattgatt tgaagagtga tcgaatggct ctacaaagac tcagggagtc
     7681 agcagagagg gcaaaaatcg aactttctac gttgatgcag acagagatta atttgccgtt
     7741 cattactgca gatacctctg gcccaaaaca tttagttgaa acaatggaaa gatcaacatt
     7801 agagcaatta gtgaatgact tagtccaaca aacaatagaa ccttgtaaaa aggctatgtc
     7861 tgatgctgaa gttagttcat ctgaaattga tgagattatt ctggtgggcg gtatgactcg
     7921 gatgccggtg gtacaaaaag cggtagagga tttctttggt aaacaggttc atcaaggagt
     7981 aaatccagat gaagttgtcg cgatcggtgc agctgtgcaa gctggtgtct tgcagggaga
     8041 cgtacaggat attttgctgt tagatgtaac cccgctgact ttgggtatag aaaccttagg
     8101 tggtgtcact accccgctga tacagagaaa tacgaccata cccacttcaa aaagtgaaac
     8161 cttcacaacc gcctctgata accaaccttc tgttgaggtg cacgtattgc aaggtgaaag
     8221 gtcaatggca aatgagaata agaccatagg aagatttaat cttgacggca ttttaccggc
     8281 ccccagaggc gttcctcagg tggaggtcac atttgatata gatgcaaatg gaatcttaaa
     8341 cgtttctgcc actgataaag gtacaggtaa agaacaaaga atcacgataa ctgctagttc
     8401 aggattatca caagatgaaa tagatcaaat gattgatgaa gctgaacaaa atgcggaaga
     8461 agatagacaa aaacgtgaaa tggtcgaaat tcgaaacacc gctcaatcgg ctgtattcgc
     8521 tgcagagact ttggttaaag aaaatgagga gcatatcgat gcctcactca aagatgaggt
     8581 ggagcaaaaa atatcagatt taaagaccgc tttagagggg gaagataatc ccgctattga
     8641 atcggccctt caacaacttc aagaatctct tcagaagata ggtcaggaag tttacagcaa
     8701 aaccggtgag gcgcctggag accaacctga ggaagaaagc tcttcggctg atgatccaga
     8761 gaatactgta gaaggtgaat ttagagaggt ctaaatgtta agagggagag aatttatttt
     8821 ctccctctta gatacgatcc tagaggacgt ggtaggccag taggtgttgc ctcatgacat
     8881 cttcggcaaa atcgttgtct acatctcgcc atacggaatg tatgtggttg gccccgtttt
     8941 gtctattgtc gaattccact acgaaatttc ctccgtgaat tctgtagtaa tgttcaccgc
     9001 tagtatcagt agccccgccc caagcaaaat gaacattgcc taatccatat ctttcaagct
     9061 cgtcaaattt tttctgtgct aattcctcgg gtacttgatt tatgtattcg gatattagag
     9121 attcaatgat ttctcgttgg gttcctgtca ttttcgagat tgacaggcct tcctcagcag
     9181 gtaaggaaac tttggagctg ttgtatgtaa ttatatcgtt cggtgcagac ccgtaaatta
     9241 tcgccttctt ttgctgccct tcatcaaagc tgctcattaa gtcgactgca atatcttccc
     9301 gatgactcag aatccgcaga ccgttttggg gcccttttct gacttcggct ggattagccc
     9361 caaagaaaaa aggtgtcata gcaatgattt cacctttcca aacactgaaa tgcagcgaaa
     9421 cgtgatgtcc ttctgcccgc caagcccaag gatcatctcc accaggtgtc ccaaatatag
     9481 tgaagtaata caattctgga tcccttttga atgatttcac gccactctct ttctcaagag
     9541 gtcccaatac gttttcaaga gcaattattt gtctagcttg ctcgtatgat ctgtcagtta
     9601 gacctgtcga catcaactta aaagctaaat ccctttgatt gggatccatg tccctcaagg
     9661 ccaaaccatg gcggttcata ggggggtagt accaaaatat tctttcccca tccagatatt
     9721 cgaaacaagc tttgcttttt tggtccggcg aaagactttc taaaaatttg gaaccggctt
     9781 cggtcatctg ttggaccaat ttgctgtgtg ctttagttgc tgccatttgt agcctcctcc
     9841 ggccggtcgt tttatatttc cttcgtgttg gttgttggac gtagattagc atctaccatg
     9901 cgcagttact agactttgac tagtttttgg aagcttctaa ggacatcagg tggagtagtg
     9961 tagtgactca tatttgttct ggctacttct gcgacatata tgctcaaatt ggaatcaaga
    10021 cacaacccat gtggagcggc gaattgaccg gttgcaaacc cataccccat atctccaatt
    10081 cgttctaata cttttccaga ttggtccaaa acgctgaccc tggggcctat atttggcaca
    10141 tttttactta cattatttcc ataacctaac tcggcaacgt aaattctttg ttgttcatct
    10201 atgtagatag cgcatggcct atgaatattt gaccaagcgg cttcgaaatt tcctctctta
    10261 tcgaaaactt gcacccgatg attctctctg tcgcagatgt aaatataacc atttttatct
    10321 gttgcaatat tgtgtatgat gttgaattca ccttcaccag ttccgggccc tccccatgat
    10381 tgcaggaggt tcccatcagg ggaatattta tgtatacgag agtttccgta accatctgtc
    10441 acaaaaatgt cacctgtttc tgggtctgtt gcggtgtgtg tacatctatt gaaaggctct
    10501 ccactaaata gtgctgaagg gttccccggg tttcctattg tcattagtac ttttccgtca
    10561 agagtgcatt gtctaacggt atgatcgcca tcatcagtgc aatacaaggt gtcatttggc
    10621 cctctactta caccatgcgc tcgtgtgaat acaccttcac cccatgattt taggaatttt
    10681 ccatctttat caaataccat catagggtgc tcaccgcgat taaaaacgta tacattgtct
    10741 tttttgtccg tggcgacacc agcggtttca gaccaagagt aatcagtagg catctgttcc
    10801 caattctcaa ctacattata tttaaaatcg ccatcgccaa ttgttacttg tgaacccata
    10861 ttaagtatcc ttgtgtgatt gttttattca gctctttttt tgatttattg gatatggacg
    10921 cgattataca acggtcattt ttatgaaaag gtgtagaatg ggtttagaag cgtaaatgcc
    10981 cctatggagg ttactgatta tggctggttc aatagtttca ttacgattat gtttgggaag
    11041 tcgagagcct atgaaagaga ttgcacaggc tgaatttctt acaggtcaag gtatgcaggg
    11101 tgacaggcat atgcgctcgg atggacttcg ttcaaagaga caggtactgg tcatggatat
    11161 agagacctta aaccattttg atcttaagcc tggtcaagta cgtgaaaacg tgactttgaa
    11221 aggattggac ctatcgacta tttccgcagg ggatagggtg tccttaggag ccgatgtagt
    11281 attggaaatc acaggagatt gtgaaccttg ttctcgtatg gatgaaatac gacctggttt
    11341 gaaagaagaa attgatggaa agaggggact cttggcgttt gttgagaaag gcgggattgt
    11401 ttcagtgggt gctgaagtag gggtaaacac cttccaacta taggggaaac ttatttaccc
    11461 tatagttaaa atcaataagt ggcgcggcct ccgcttatgt caaatacagc cccggtactg
    11521 aaagagcatt cactagacga tagccaagcc accatagcgg ctatttcaga aatttcaccg
    11581 gtcctcccca caggtatcct atcgaccatg tattgaactt gctccggagt aaattcctct
    11641 aatatacgag tttggacgac tgcgggagta acacaatttg ctaaaacccc cgtgcctgct
    11701 agttctttgc ctatagattt tgtaaggccg atcactgcag ctttagtgga ggaataggga
    11761 accatacggg ggtttccttc cttaccggca atagaggcaa tgttaacaat tcttccataa
    11821 tctttttcca acatatgagg aatcacttcg cgacaaaacg aaaagactcc tcgaagatta
    11881 gttctgtata ccctatccat ttcctcgatg tctaactccc aaatatttcc gttaactcca
    11941 cctatccctg cattatttac taatatgtct atctttcccc atttattgat tgctttactg
    12001 actgcttctt gagctgtttt aggctcagaa acatctccaa tggaaatttc cacttcctga
    12061 gttctggccg taatttcact agctgattgc ttcatcatgt tggcgtctaa atctaccatt
    12121 accaagttcg ccccatcgtt ggcaaggcgt ttggctattc cgtaaccaat tcctgtggcg
    12181 ccgccggtga caatggctac tttaccctct aggttattca tattaatctc ctaatttaat
    12241 cttggaattt aatgaaaagg acaaacccac aaagcagctt tttcttccca ttcatattga
    12301 cttggtgagg gtcctttctt ttttaccgtg ctatcagcta atccgtaggc tgtggaaaac
    12361 atcaatgcca ctaggatgat tagcaacgca aatgggaata taaggggtat ttctttcata
    12421 taaattatgt ttgtctcttc ttcataaacg tcattttctg gaagtcctat cataccgatc
    12481 ctcaagtctg acaatgtcat cttctccaaa gtatgagcct gattgcacct ctattatttc
    12541 gagattttcg ccctcgtcat tttccagtct atgctgcact cctcttggta tgtaggtaga
    12601 ttgattcttt tttagggtca ttgtttcact tccccttgta attgttgcaa ctccagatac
    12661 gatcacccaa tgttctgttc tatggtgatg tatttgcagt gaaagcgcat gttttggtaa
    12721 gacagttagt ctcttaacct ggaattcttt tccactatct aaaatttcat aatgccccca
    12781 tggtcgaaat tcttttttgt ggctttcgaa aatcgctgtg ttttcagctg ggattttatc
    12841 aactaaatct ttaattctgt gttcctgatt tctatctgct accaatattg catctggtgt
    12901 ctctacaaca ataaggttgt caacatgaga agccgcaatc gttctgtgat cgctaatgag
    12961 tatggaattt tggacagagt cggtaatgac ctccccgtgc agcaggttgt ttgatttatc
    13021 gggattttga gagctcatga aagaacccca cgatcctaaa tctgaccata cggattttaa
    13081 gggaatcacc caatttttaa aaatatttcc aggaaattcc agattctcca tgactgcatg
    13141 atctatagat ttgcttggac agtcatttag ggatttcgat tcagggcgaa aaaatatgcc
    13201 atcttctacc ccattttgaa tggacctttc acaggcctcc agaattcttg gagagtggat
    13261 ttttaagtaa tgaatccaag aacttgcttt tacaagaaac atcccactgt tccaaaagaa
    13321 ttctttggat tcatgcattc tccgtgcggt cttagaatct ggcttttcat ggaacctctt
    13381 caattcatag tagttatttt ttgttgaatc acctttttga atataaccaa agtttacgtc
    13441 aggggtttgc acatcgattc caaatgtaac tattcctccg tcttccgcta atttagaagc
    13501 agtatctaca acctcaaaga aattttcatc ttcttctatg aaatggtcgg atggcattga
    13561 aaataaaata tggtctccaa aatttcttaa taagtagagt gctgcaagcg ttaaggcggg
    13621 agcagtattt ttagggaatg gttctaaaag gattttttca actggagctg aaatggaatt
    13681 caagtggttg gttagcagaa agcgattttg ttcattacac acgatcacag gcaaagccaa
    13741 tcccaagttc tgtgatcgct taacggtgtt ttgaattaaa gatgtttgac tattattggt
    13801 gagaaaaggc ttagggtatt gatttcggga cattggccac aatcgtttac ctgaaccgcc
    13861 tgccaatata actggtttca atttcacaat tggtagaacc tctcacaggc gcagtaaaaa
    13921 gtaaggatta tttactgcag attataaatt tttatcgata aatgggtcaa caaattgaat
    13981 agatatcttg tgtgcagatg actttgtaaa ataaaatcat ctaatgtatt cctattggcg
    14041 gatagggtaa tcagattgtt gaaggaatta tataaggaga aacgttatgg gtactttgga
    14101 tgggaaaact gtcgttgtca ccggtgcgag tcgaggcatt ggggcagaaa ttgccaggat
    14161 ttttgcgaaa gaaggaggaa aagtagtttg tgcagcgagg actttaaaag aaggagatca
    14221 tcctttggaa ggttccttgg aaaaaacagt gagtgatata aatgacgctg ggggcgaggc
    14281 taaaccagtc atcgccaata tatcactgcc tgaagattgc gaacgccttt ttgctgagac
    14341 tcatgaatta tatggaaagg tcgatgtgtt ggttaataat gctgccttga catattttat
    14401 tcctgtgagg gactacccag ttaaccgctg gatgaggtct tgggcagtca atttccacgc
    14461 tccatttatt ctaagccaat tagccttgga agatatgatt tcaatgggat ccgggagtat
    14521 agtgaacatt tcttctggag cggcgattgg tccaggccgc ggtccctatt caaatgttcc
    14581 gccaaactct ggtggtactt gttacggggc cgagaaggca gctttagaaa gatttaccca
    14641 aggattagct caagaagtat atggagatgg aatttccgta acttgtgtgt ctccatccca
    14701 ggtagtgcct acacctggaa ctgtttttca taatttggta accggaatgg atgacccacg
    14761 aggtgagcat ccggaattga tggccaaggc ggcgttatta ttggccaccg aaccgatgga
    14821 taaagtaacc ggtagagtga catatagtca gcagattctt aaagaatttg ggtggataga
    14881 tgaagccaaa ggtacagggg ttgatcagga tcgagtgggt agcgggtata gccaagtata
    14941 atttccgctc caccggtgga ggccgagacg gtaataccaa gctttaaaca tggtatataa
    15001 atgccttgcc tttgaggtcc ctctgtggtt agtttgaact ggccattcat ataatgggga
    15061 cgtgttctct atggtaagga atttataagg taatataaag tgcaaatcgt agaggtggag
    15121 aagctatgaa actaactcaa gctagggtaa tcaactacag aagtattgac gattcctcat
    15181 gggttagagt tgatgatgtc acggctctgg ttggtaaaaa tgagtctggt aagacagcat
    15241 ttttgcaggc tattaggaag atcaattcta tttctggtga agaagatact tttagtatta
    15301 gggattaccc tcgaaaaggt tacatcaaat ataaaaaaat acatgatcaa aacccatgtg
    15361 aagtagcaca ggcagaattt gagctgaatg ccgaggaaat atctgaaatc gaggcgaatt
    15421 ttggcgacgg catattggct tcaaacaaag tgattgtcac taaaaactat aaaaatgaac
    15481 gaaattggaa aattagcctt gcggaatcgg tttcacaacc tcccaacgtg acgactttta
    15541 ctcaaccttc cacacctgct ccaacttctt ttcaaagtgc tgtgcaggct gtggatgaag
    15601 ttagtaatgt ttctgagaga atttcaaagc aatttctaga aaaatggctc cctaaatttg
    15661 tttacttcga taattacagc ctcatgagag gaaaaatatc tatcaacgag ctcaggcaga
    15721 gatcagaaaa tggagggcct tttgatgatg ccgataggac attcctgtct cttttgactt
    15781 tatctggtgt ttctctagag gatttggaaa aagatttagg gtatgaggac ataaaagtcg
    15841 aattagaatc tgcctcaata accataactg atgagatatt tgaatactgg caacaaaatc
    15901 gccaactaaa agttgaattt gatgtgtccc aggccgatcc tagggatgct gccccattaa
    15961 atcaagg
//
LOCUS       DASCNS010000208        10572 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001589415, whole genome shotgun sequence.
ACCESSION   DASCNS010000208 DASCNS010000000
VERSION     DASCNS010000208.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10572)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10572)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10572
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001589415"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="QF838_07645"
     CDS             complement(<1..591)
                     /locus_tag="QF838_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60589.1"
                     /translation="MGWKVLITDHVWPTTDPERAVLEAAGAEVIVAPDGDEDTLIELA
                     RDVDAIMTCFAKVTGNVIRSAKNCVSIGRFGVGVDNIDVSTASELGIPVTYVPDYCVD
                     EVSDHVLAMLHTWNRKIALFDRSVKSDGWGHLGLTMRIKRLRGKTIGIVGFGRIGQAV
                     AEKAAVFGLNILASDPFTPKEIVESSGGKLVSLEELL"
     gene            complement(625..2253)
                     /locus_tag="QF838_07650"
     CDS             complement(625..2253)
                     /locus_tag="QF838_07650"
                     /inference="COORDINATES: protein motif:HMM:NF025925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HJO60590.1"
                     /translation="MSPVFANGKEYPTGPQRTIFDYADDLEVRVPTACGRNGECHECV
                     VEIKKGMESLSQPTKEETFLRGDYRLACQAVVEDLNSNVEFTTLRRQPKILTSGVKRP
                     VSLESVVTKRGDRVFIEDMDEDRYQGHILGLAGDIGTTTIVLSIIDLESGDILTTSSF
                     ENPQRFGGSDVMNRISYDGGPNKGELKKVLLSSINYEIGEMLREHKIHRRRIYDAVLV
                     GNTTMRDILFGVNVQSVGEKPYKSIIQHAMESGSRESTAINISAKELGLRIFPQARIY
                     SGPLIGSHVGSDVAADLLAIMADEAEHPVMLVDIGTNTEVVIGTRDKMVAASCPAGPA
                     FEGGEITYGMPGYEGAVESVKIQDGILKIATIGDASIQGICGSGLIDLLAELRKYNLM
                     TDLGVYSNGENAYIFSEEENMALYRSDISALAQAKSANYCGQYLALRHFGAPISKISK
                     LYLAGGFANYINSSNARDIGFIANFPIKKIEKVGNASLEGAILMLKSMRMRMEIEKLV
                     LDIDHLELETVPDFFEVFVEGCMFKSMPRDLTSI"
     gene            complement(2614..3372)
                     /locus_tag="QF838_07655"
     CDS             complement(2614..3372)
                     /locus_tag="QF838_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HJO60591.1"
                     /translation="MPVTNEDLKAELEYVTNSAEQQHIRNLWNEADPLMQDISVSLIK
                     GDNNRVDQLTQKALEAGFSANTILDDGLIAGMAIVGVKFRDNLIFVPEVLVAARAMKA
                     GMTHVEPILSASGIEPVGTVIMGTVKGDLHDIGKNLCIMMLRGAGFSVIDLGVDTSPE
                     EFIEAVEENEAGVLGMSALLTTTMPNMGRTIDAFEEIEMREVVKIMVGGAPLSQEFAD
                     DMGADGYGKDAISCVELAKTFMSPDTEPSISPLG"
     gene            complement(3481..4518)
                     /locus_tag="QF838_07660"
     CDS             complement(3481..4518)
                     /locus_tag="QF838_07660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004889.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HJO60592.1"
                     /translation="MPYQETMSSKERVMNALAGKPVDRTPAVNPTNVATVELMDLVDA
                     PFPYACQDGELAARLAATGHTELGFDSVSPYFSIIQESSALGCEMQWEEKDNWPTVRM
                     YNPIWKSADDVKIPANILEHRDMKAITDSIKMLKNEFGDEVAVIGKTMGPWTLAYHVF
                     GVESFLLGTVDNPDETKRALQKLKEVSVLFGQAQIDAGADALTFPDHATGDLVSGEYY
                     KEFLQDIHTEMVDVLEAPLILHICGKTVDRMPYIAQTGMASFHFDSKNDPQEAMDAVD
                     GGITLVGNINNPETLYARGPEEVRQEVYKCLEAGVQMIGPECAVPLATKIENLMEIPL
                     AIKDWSAERHG"
     gene            complement(4537..5211)
                     /locus_tag="QF838_07665"
     CDS             complement(4537..5211)
                     /locus_tag="QF838_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60593.1"
                     /translation="MKLVSYDGPRPKDVIEKYGRCLELVPTDKRFNEISVGLYVKNSV
                     FTVWSFSTKPGVENRIEEIRGLLVSLGGMTPVLGTHNQAKFECGLIHSRPVRFLLAQA
                     VGKDPEYATSPSAMTIKDSKSNLIINAEGFDRDNTRCYQISVNGEAKNPAMRLRMIVA
                     GFSKYGEMDKMGDQEVAFECRNNHDELLRILLPYSRNVSSVETMMQAESMRGQMTTST
                     LGFSQT"
     gene            complement(5214..6116)
                     /locus_tag="QF838_07670"
     CDS             complement(5214..6116)
                     /locus_tag="QF838_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014615.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HJO60594.1"
                     /translation="MNLKSQSILDRISNGEMLVSDGATGTFLQQNGLEPGGCPEEFNV
                     SHPEVVQRMAKSYFDAGSDMVLTNSFGGTKFRQTHYGFGDKVDEFNRLAAEHAKSQSK
                     PGTFVCGSVGPSGEFLEPLGIVSEKELYRAFRSQVTSLELGGADGVVVETMTALDEAT
                     IAIRAAKENTNLTVISTMTFDKGPRGYFTMMGVTPEQAAIELVKSGADIVGTNCGNGI
                     DNMIEIATMMRDATDSPLLVHSNAGIPSLKDGEILYPEPPDYMAEKFMRLKKIGVNII
                     GGCCGTGPEHISAFSTLLGKKQGV"
     gene            complement(6132..6458)
                     /locus_tag="QF838_07675"
     CDS             complement(6132..6458)
                     /locus_tag="QF838_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025142.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HJO60595.1"
                     /translation="MYWKEIPVQVQATNESETQSLPLESRFQEAVDAISMLDGSYGSD
                     EYLDAWEWGEYFEMPGELNHVLKSIGDRYNNGMPQNFVAKLRDLHKNGSRNPSAGSID
                     GWIESN"
     gene            6615..7475
                     /locus_tag="QF838_07680"
     CDS             6615..7475
                     /locus_tag="QF838_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60596.1"
                     /translation="MPHAPINGIDIYYEVHGTGDAIIFCHEFAGDIRSWDLQVNHFSR
                     NFQVITYCARGYSPTSVPEDPKLYTQDLVIDDLKGLMGFLKIDKAHIVGLSMGGNVAL
                     NFGIKYPEQAKTLTIAGTGTGSDNPDLFRGRILKFSDGMRSEGMAFMSDYLKGPQRVQ
                     HQLKDPKGYQLFCEKFLEHSNIGSANTFYGIMAKRPPIFDLKNQLSNMKIPVLVMTGD
                     EDDPCIKPSLFIKESIPSAGLIMFPRSGHAINLEEPQLFNASVTEFINHSGASYWGPR
                     GQGEAEGSLT"
     gene            complement(7664..8770)
                     /locus_tag="QF838_07685"
     CDS             complement(7664..8770)
                     /locus_tag="QF838_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60597.1"
                     /translation="MKITDITTTHLHSPEGKPIQDATIPTPKEGSGGRGQIFVHIKTD
                     ADIEGLGMTYASPGVIDVIESSLKQELIGKDPLDIEKLWNDMYWKVRGYGRKGIAFCA
                     ISAIDIGLWDLKGKYVNLPLYKLLGAYTDSVPVYGSGGWTNFSEKELVEEMTGYVSDG
                     IPRVKMKVGKDFGLSEREDIERVAAVRKAVGDDVSIYIDANNGYYRKQAIYMAKEFEQ
                     FQIGWLEEPLIPDDIDGMAEISKATTIPIATGEHEYTKYGFRELIARGGVDIVQPDIG
                     RVGGVTEWVKVAHLAHSFNLPVAPHALQLAHLHVACATPNLKVVEYMNVSLEGDELWY
                     VDFPKQKDGYWSPFHDKPGLGLELDHYSIVKYQI"
     gene            complement(8796..10466)
                     /gene="argS"
                     /locus_tag="QF838_07690"
     CDS             complement(8796..10466)
                     /gene="argS"
                     /locus_tag="QF838_07690"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HJO60598.1"
                     /translation="MTTVPDMLRNSITNAFELAQKDNLIAVAKITDAAVERPQNPEHG
                     DYASSLPLKLAKPLEKNPMEIANVLAQYLKEDEILDDVVVAKPGFLNFVLSKKWLQNQ
                     VDQILAEPENYGVTNVGNGKRFQVEYISANPTGRLHVAHARGAVIGSTLASVLKSSGF
                     DVEQEYYLNDAGAQIDKFNRSLMTRYMQSLGNDIDMPEDSYPGEDVTAVAEKIRDSSH
                     HRYDTSNPKEISELGKVGIDLMIEGIKEDIESLKIHFDNWFSEKSLFENGQFEKCMAL
                     LKEMGFVSDKDGATWLLNTKLGEEKDNVLIRSNGTPTYFSTDIAYHYNKFVKRKFDTV
                     IDIWGADHQGQVPRLKSAMKMLGSNPENLEMILVQMVRFKKGETTEKLSKREGNVVPL
                     IELVNEIGPDACRFMFLSRSHETQLDFDLDLAKKQSSENPIYYIQYGHARICSILDLA
                     IEKKIDYSTGLVAMLGHESERQLINKILELPSIMNAIADTYEVHHLPHYSLELATAFH
                     NFYQNCRVISGNEGDLELSKARLKLCEAAKLSLARCLDLMGMSKPEKM"
BASE COUNT         2739 a         2473 c         1986 g         3374 t
ORIGIN      
        1 cagtagttcc tcaaggctta ccagtttacc gcctgaagat tctactattt ctttaggagt
       61 gaaagggtca gatgccaata tgtttaaacc gaagacagcg gctttttctg ctactgcttg
      121 tccgatccga ccaaatccca caatgccaat agttttacct ctcagtcttt tgatacgcat
      181 cgttaaaccc aaatgacccc aaccgtcaga ttttactgat cgatcgaata gggctatctt
      241 tctattccaa gtgtgtaaca ttgctaaaac gtggtcagaa acttcatcta cacaatagtc
      301 tggaacgtag gtcaccggta taccaagttc gcttgctgta ctcacatcta tattgtcaac
      361 tccgactcca aatcttccta tcgatacaca atttttggcg gacctaatta cattccctgt
      421 gacttttgca aaacatgtca tgatcgcatc cacgtcccta gctaactcaa tcagagtatc
      481 ttcgtcccca tcaggggcta ctatgacctc cgctccggca gcctctaaaa cagccctctc
      541 aggatctgtt gttggccata catgatctgt aataaggact ttccaaccca tttatttctc
      601 cctttcctta cgattaacta acaactaaat tgatgttaaa tctctcggca tggatttaaa
      661 catacaccct tcaacaaaaa cttcgaagaa atcaggaaca gtttccaatt ccaaatggtc
      721 gatgtccaat actaactttt ctatctccat tctcattctc attgatttaa gcattagtat
      781 tgcgccttcg agcgaagcat ttcccacctt ttcgatcttt tttatgggaa aattggctat
      841 aaagccaatg tcccgagcat ttgatgaatt tatgtagttt gcaaaacctc cagccaagta
      901 tagtttggat attttactaa ttggtgcacc gaaatgccgt aacgctaaat attggccaca
      961 atagttggca gattttgcct gggccagggc actaatatcg gatcggtaga gagccatgtt
     1021 ttcttcttcg gaaaatatgt atgcattctc tccatttgag taaactccta ggtcggtcat
     1081 aagattgtat tttctcagtt cggccaacaa atcgatcaac cctgatccac agatcccttg
     1141 gatacttgca tcccctatag tagcgatctt caaaatccca tcttgaattt tcacggattc
     1201 aacagctcct tcgtatccag gcatcccgta tgttatctcg ccaccctcaa atgctggccc
     1261 agcagggcag gaagctgcga ccattttatc tcgagttccg attacaactt cagtattggt
     1321 gcctatatcc actaacatta cggggtgttc ggcttcatca gccattatcg ccagtaaatc
     1381 tgctgccaca tctgacccta cgtgagaccc aattaaaggt ccactataga ttcgagcctg
     1441 ggggaatatt cgtagaccaa gttcttttgc acttatatta attgcggtgg attctcgtga
     1501 accagattcc atagcatgtt gaataatcga tttgtagggc ttttccccta cagattggac
     1561 atttacacca aaaagtatgt ccctcattgt agtgtttcca actagtacag cgtcataaat
     1621 tctcctacga tgaattttat gctccctaag catttcacca atttcgtaat tgatcgagga
     1681 tagcaatacc ttttttaatt cacctttatt tggcccacca tcataggaaa tcctgttcat
     1741 tacatcggac ccgccaaatc tttgcgggtt ttcaaaagag gaagtggtta atatgtcacc
     1801 actttcaagg tctatgatgc ttaatactat ggttgtggtt ccaatatccc cagccaaacc
     1861 taatatgtgc ccctgatacc tgtcttcatc catatcctct atgaagaccc tgtcacctct
     1921 tttggtaacg acgctttcta aactaacagg tctcttcact cctgaggtta atatttttgg
     1981 ttgccttctt agcgtggtaa attcaacatt ggagtttaaa tcttcgacta cagcttggca
     2041 ggccaacctg taatcacctc ttaagaaagt ttcttctttc gtcggttggc ttagggattc
     2101 catccctttt tttatttcca ccacgcactc atgacattcc ccatttctac cacatgcggt
     2161 tggaactcta acttcaagat catcagcgta atcaaatata gttctttgag ggcctgtcgg
     2221 atattctttg ccgttggcaa aaactggaga cattggttat accttctaac tgtatttggc
     2281 taggtggact ctacagtgag caaagaaaga atgtgctttt agtcttcata ttaattacta
     2341 ggttcccacg ccgagcgata atcgaggtca tcacttccga gacaaagata caattttcca
     2401 acagagcccg gcctatcttg gttgcggcaa ccaaatttcg cggtgcatct gtcgtcaaca
     2461 tttttgtacg ggcatctcca ggtgaatact tccgaggctg tatctgagat agacttgaag
     2521 attgattcaa gctttcccaa acttttccta tatttttctt ggtcgaccgg ttccatatta
     2581 ttgtctcagt agccacaaaa tactttataa gacttaacca agcggactta ttgaaggctc
     2641 ggtatcgggg ctcatgaaag ttttagctaa ttccacacag gatatagcat cttttccgta
     2701 accatccgca cccatatcat cggcaaattc ctgggaaagg ggggctccgc caaccataat
     2761 tttcaccacc tcccgcatct caatttcttc aaaggcatct atggttctac ccatattagg
     2821 cattgtggtt gttaataatg cagacattcc taaaacacca gcctcattct cttcaactgc
     2881 ctctatgaat tcctctggag aagtgtctac gcccaaatcg ataacactaa agccggctcc
     2941 tctcaacatc atgatgcaaa ggttctttcc aatatcatgt aaatctcctt tcactgttcc
     3001 cattatcaca gtaccaacag gttctatacc tgaagccgat agaattggtt caacgtgagt
     3061 catcccggct ttcatagctc ttgcagccac aagcacttct ggtacaaata taagattatc
     3121 tctgaatttc acgccgacaa tcgccattcc tgctatgagg ccatcatcca gaattgtatt
     3181 ggctgaaaac ccggcttcta gagccttttg ggtcaattgg tcaactctat tattgtctcc
     3241 ttttatcaaa gaaactgata tatcttgcat taagggatct gcttcgttcc acaagtttct
     3301 aatgtgttgt tgttctgcag agttggttac atactctaat tccgccttca aatcttcatt
     3361 agttactggc atatcgtaaa cgaacctctg atactgattg ggctatggtt tattagaaat
     3421 tggacataaa tttacattca aggggagcaa ttatacgact aaattataat cggacatagc
     3481 ttacccgtgc ctttcagcag accaatcttt gattgctaaa ggaatctcca tcaggttttc
     3541 tattttggtc gccaatggca ctgcgcattc tggaccaatc atctggactc cagcctccaa
     3601 gcatttgtag acctcttgtc ttacttcttc agggcctctg gcgtacaaag tttccggatt
     3661 atttatattt ccgacaagag tgatgccacc atctacagcg tccatagcct cttgaggatc
     3721 atttttagaa tcaaagtgaa aagaggccat tcctgtttgg gcaatatatg gcatcctatc
     3781 cacggttttc ccgcatatat gcaaaatgag aggtgcttcc aacacatcta ccatttcagt
     3841 atgaatatct tgtaaaaatt ctttatagta ttctccactc actaaatcac ctgtggcgtg
     3901 atcggggaat gtcagtgcat cagctcctgc atctatctgg gcttgtccga aaagtacgga
     3961 aacttccttc aatttttgta aagccctctt tgtttcatct ggattgtcca ctgtgcccaa
     4021 taaaaaactc tctacaccaa aaacgtgata ggcgagagtc catgggccca ttgtttttcc
     4081 aataacagct acctcatctc caaactcatt tttcagcatt ttaattgagt cagttatagc
     4141 tttcatatct cggtgttcca gtatgttggc cggtattttc acatcatcag cggacttcca
     4201 aatggggtta tacattcgga ctgtgggcca attgtctttt tcttcccatt gcatttcaca
     4261 tcctagggcc gatgactctt gtattatgct gaaatagggt gaaacggagt cgaaacctaa
     4321 ttccgtatgg ccagtcgctg ccaacctagc tgccaactct ccgtcctggc atgcgtatgg
     4381 gaaaggagca tctaccagat ccattaattc cactgtcgca acattggttg gatttacggc
     4441 tggtgttctg tcaacaggtt taccggctaa agcgttcatt accctttctt tggacgacat
     4501 ggtttcttga taaggcattt ctcaaccgct ccttctttat gtctgactga aacctaaggt
     4561 actagtggtc atttgacctc tcatggattc tgcttgcatc atcgtttcga cactacttac
     4621 gttccgcgaa taaggtagca gaattcttaa taattcatcg tgattattcc ggcactcgaa
     4681 agccacttct tgatctccca ttttgtccat ttcaccatat ttggaaaatc ctgccacaat
     4741 catcctcagg cgcatagctg gatttttagc ttcgccatta acagatatct ggtagcatct
     4801 ggtgttgtcc ctatcaaaac cttctgcgtt gatgattaaa tttgatttgc tatcctttat
     4861 cgtcattgcg gacgggcttg tggcatattc cggatctttt ccaacagcct gagccaatag
     4921 aaaccggaca ggcctgctat gaattaaacc gcactcaaat ttggcttgat tatgcgtccc
     4981 aaggacagga gtcatgcctc ccagagagac taataagcct cgaatttctt ctattcggtt
     5041 ttctacacca ggtttggtgc tgaatgacca cacagtgaag acagaatttt ttacgtataa
     5101 gcctacagat atttcattaa atcttttgtc cgtgggaact agttccagac agcgaccata
     5161 tttttctata acgtcttttg gtctcgggcc atcgtaggac acaagtttca tgtttatact
     5221 ccttgttttt tccccaatag tgttgaaaat gcggagatat gctctggtcc ggttccacag
     5281 catccaccaa tgatattgac tccaattttt tttaaccgca tgaatttttc ggccatgtag
     5341 tcgggtggtt caggatacag tatttctcca tctttgagcg atggaatacc agcatttgaa
     5401 tgaaccaaga gtggagagtc agtagcatct ctcatcatag ttgctatctc tatcatgtta
     5461 tctattccgt ttccgcaatt ggtccctaca atatcggccc cagatttgac taattctatt
     5521 gcggcctgtt caggtgttac gcccatcatt gtaaagtacc cccttgggcc tttatcaaag
     5581 gtcatagtcg atattacggt taaattggta ttttctttag cagctctgat agctatggtg
     5641 gcttcatcaa gggctgtcat agtttctaca accactccgt cagcaccgcc taattctaag
     5701 gaagttacct gactacgaaa ggcccggtaa agctcttttt cagagactat ccccaaaggt
     5761 tctaggaatt caccacttgg gcctacagac ccacacacga aggttcctgg cttcgattga
     5821 ctttttgcat gctcagctgc gagcctatta aattcatcta ctttgtctcc aaatccgtag
     5881 tgggtttgcc gaaatttggt gccaccgaaa gaattagtga gaaccatgtc agatcctgca
     5941 tcaaagtagg attttgccat tctttggaca acttctgggt gacttacatt gaactcttca
     6001 gggcatccgc ccggctcgag gccattttgt tgtaaaaaag tcccggtagc tccatctgaa
     6061 actaacatct ctccattgga tattcgatca agtatgcttt gggattttaa attcatcttg
     6121 tttttggcct tctaatttga ttctatccat ccgtcgatgg atcctgcgct tgggtttcga
     6181 gatccatttt tatgtaaatc cctcaacttg gccacaaaat tctgcggcat cccattgttg
     6241 taacggtctc cgatggactt caacacatga ttcaattccc cgggcatttc aaaatattca
     6301 ccccactccc atgcgtcaag gtattcatct gaaccataac ttccatccag catagaaatc
     6361 gcatctacag cttcttggaa tctcgactcc agtggaaggg attgggtttc agattcattg
     6421 gttgcttgca cttgtaccgg aatttccttc caatacatta ttcgcacttt caaatttcta
     6481 atcctagggg aacgtaatca cagctattat agttccatca gcaaacatac agaagccgca
     6541 gcctccatat gcctatttct aaccgatgtg ttataaatca ataattctta atagtgttcg
     6601 tcaaggaggg ggttatgcca catgcaccga tcaacggaat agatatttat tatgaagtcc
     6661 atggtacagg tgacgcgatt atattttgtc atgaatttgc aggagatatt cgatcgtggg
     6721 acttacaagt aaatcatttt tccagaaatt tccaagtaat cacttactgt gctagaggct
     6781 attctccaac ttcagtacct gaagacccaa aactatacac ccaagatctg gttattgatg
     6841 accttaaagg attgatgggt tttttgaaaa ttgataaagc tcatatagtt ggtctttcaa
     6901 tgggaggaaa tgttgcccta aatttcggca ttaaataccc ggaacaagcc aaaacattaa
     6961 caatagctgg gacggggact ggttcagata atcctgacct tttccgggga cgcattttaa
     7021 aattttcgga tggaatgagg tcagaaggta tggcttttat gtcagattac cttaaaggcc
     7081 cccaaagggt tcagcaccag ttgaaagacc ctaaaggcta tcaacttttc tgtgagaaat
     7141 tcttggaaca ttccaacata ggctctgcaa ataccttcta cggtattatg gccaaaaggc
     7201 cgcctatttt cgatttaaag aatcaacttt ccaacatgaa aattccggtt ttagttatga
     7261 ctggggatga ggacgatccc tgtattaaac cgtctctttt tatcaaagaa tctataccta
     7321 gtgcaggctt aataatgttt ccaagatccg gacatgcaat taacctagaa gagccacagt
     7381 tgtttaatgc ttctgtaact gaatttatca accattcagg agcctcctac tggggaccac
     7441 gtggtcaggg tgaggcagaa ggatctttaa cctaacagaa ttttgagttg atggctaaaa
     7501 ttcctgctgg gactcaacaa aaataaacat ttgatgttca cacttttttt gaaatcgttg
     7561 cggtaataaa taatttccta gatatgaaag ttagcgcctc cggtcatatt ctgtcagctt
     7621 gtaacgcacc ggccaaattt ctatgggtaa ataggcttta atgttagatc tgatacttga
     7681 ctatcgaata atgatcgagt tccagtccaa gacctggttt gtcatggaat ggagaccaat
     7741 aaccatcctt ctgttttggg aaatctacgt accataactc gtccccttct agagacacat
     7801 tcatgtattc aactactttc aaattcggtg ttgcacatgc tacatgtaga tgggctaatt
     7861 gtagtgcgtg tggggctact gggaggttaa atgaatgtgc cagatgtgct actttcaccc
     7921 actcggtgac accaccaacc cgtccgatat caggctgaac aatatccacc cctccgcggg
     7981 cgataagttc cctaaaacca tattttgtgt attcgtgctc cccggtagct ataggaatag
     8041 tagtggcctt ggatatttcg gccattccgt caatatcgtc aggtatcaac ggctcctcaa
     8101 gccagcctat ctggaactgt tcaaactcct ttgccatgta tatggcttgt tttcgataat
     8161 agccgttgtt tgcatcgatg tagatactga catcatctcc taccgctttt cttactgcag
     8221 ctactctttc aatatcttct ctttccgata gaccaaaatc cttgcctact ttcattttga
     8281 ctcttggtat gccatcggac acataacctg tcatttcctc gacaagctct ttttctgaga
     8341 aattggtcca gccgccgctt ccatagactg ggactgaatc tgtatacgct ccaagtaatt
     8401 tataaagtgg tagatttacg tattttcctt tcagatccca tagccctata tcgatggcag
     8461 aaatagcgca gaatgctatc ccttttcggc catatcctct cactttccaa tacatatcgt
     8521 tccatagttt ctcaatgtcc agggggtctt tacctatcag ttcttgtttg agagaacttt
     8581 cgatgacatc aataacccct ggcgaagcgt aagtcatccc gaggccttcg atatctgcat
     8641 cagttttaat atggacgaaa atttggcctc ttcctccaga cccttccttt ggtgtgggta
     8701 tggtggcatc ttgtattggc ttcccttcag gactgtgaag atgagttgtg gtgatgtcag
     8761 ttattttcat tttggccctt ttataattgg agaatttaca ttttctcagg cttactcatt
     8821 cccataaggt ctaggcagcg tgcaagtgaa agtttggccg cttcgcatag tttcaatctg
     8881 gctttactta actccaggtc gccttcattt cccgaaatca ccctacaatt ctgataaaaa
     8941 ttgtgaaaag ccgttgctaa ttctagtgag taatgtggca agtgatgcac ttcataagtg
     9001 tctgctatcg cattcatgat agacggcaat tccaatattt tgttgatgag ttgcctttct
     9061 gattcatggc ccaacatggc aactaagccc gtggaataat ctatcttttt ttcgatagcc
     9121 agatctagga tgctgcatat tctggcatgc ccatattgaa tataataaat ggggttttca
     9181 gaagactgtt ttttagctaa atcgaggtca aaatctaatt gagtttcgtg ggatcgggac
     9241 aaaaacatga acctacatgc atccggacca atttcattga ctagttctat caaaggtaca
     9301 acgttgcctt ctcgtttgga taatttttcg gtggtctcgc ctttcttgaa tctaaccatc
     9361 tgaaccaaaa tcatttctag attttcggga ttactgccaa gcattttcat tgcagatttt
     9421 agtctgggta cctgcccttg gtggtcagca ccccaaatat cgatcactgt gtcgaatttt
     9481 ctcttcacaa acttgttgta gtggtaagcg atgtctgtgg aaaaataagt tggcgttccg
     9541 ttgcttctta tcaggacatt atctttttct tcgcctaact tggtatttaa gagccatgta
     9601 gccccatctt tatctgatac aaatcccatt tctttcaata aggccataca tttttcaaac
     9661 tggccattct caaaaagact tttttcactg aaccagttat cgaaatggat ttttagggat
     9721 tcgatatctt cctttattcc ctctatcatc agatctatac cgaccttacc tagctccgat
     9781 atttctttag gattagaggt gtcgtatcta tggtgactcg aatctcttat tttttcagct
     9841 actgccgtca catcctctcc tggatatgaa tcttccggca tatcaatatc attccccaac
     9901 gactgcatat atctggtcat taaggatctg ttgaatttgt ctatttgagc tcctgcatcg
     9961 tttagatagt attcttgttc gacgtcaaaa ccggatgact tcaaaacgct agctagtgtg
    10021 cttcctataa ccgctcctct agcgtgagct acatgcaatc ttcctgttgg atttgcgctt
    10081 atgtattcga cttgaaatct tttgccattt cctacatttg tgaccccata gttttccggt
    10141 tcagccaaga tttggtcaac ttggttttgt agccactttt tgcttagaac aaaattaagg
    10201 aaaccgggtt tagccactac aacatcgtcc aggatttcat cttctttcag gtattgagca
    10261 agtacattcg ctatctccat aggatttttc tccaatggtt ttgcgagctt caacgggagg
    10321 cttgaggcat agtcgccatg ctcagggttt tgtggtcttt caacagcggc atcggtgatt
    10381 ttggccacag ctataagatt gtccttctgt gctaattcaa aggcattcgt tatcgaatta
    10441 cgcaacatgt caggaacggt ggtcaatttc acaactccag taaactttga tctatttatc
    10501 caattacatg tatcgtaggg tcgttttctg aaggactgta aacttcccct acgatttgat
    10561 tactcagagt cc
//
LOCUS       DASCNS010000209         7611 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001604600, whole genome shotgun sequence.
ACCESSION   DASCNS010000209 DASCNS010000000
VERSION     DASCNS010000209.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001604600"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="QF838_07695"
     CDS             complement(<1..1205)
                     /locus_tag="QF838_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HJO60599.1"
                     /translation="MHIGIDVGGTNTDAVLMNGDVLVDKIKNPTTPDVTSGIISCINT
                     LISSRSDVDQIDAVMLGTTRFVNALLQRRELAPSAALRLCLPATTMLPPLVDWPDDLK
                     NSIGGHTYMAGGGHEYDGREISSIDEKELRKIANQMTDEGVRSISICGVFSPVDGSHE
                     QIAAEIIKSETPDVNITLSSEIGRVGLLERENAAMLNACLVEVASKTVAAIRTAMLSA
                     GLNVPLFFSQNDGTLMQSEFASHYPVFTIASGPTNSMRGAAFLSGVLDAVIVDIGGTS
                     TDGGMLIRGFPREAAVSVDVAGVRTNFRMPDVFSTALGGGSLIKQNPLTIGPESVGFK
                     LASESMVFGGGQMTATDIAVANGMAEVGDLRLVSNIESSFAEGTINEIQKRVEDVVDQ
                     VKISAHPVPV"
     gene            complement(1262..2335)
                     /locus_tag="QF838_07700"
     CDS             complement(1262..2335)
                     /locus_tag="QF838_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HJO60600.1"
                     /translation="MWHISEDDLESIAIGAGILGTGGGGNPYIGMLRARQMIRENGPV
                     KVLSPNELDENDNIVCVGGIGAPTVGIEKVRDKQSYYALKAIEDFTGKKATAIISNEI
                     GGSNSLEPIIPSALTGLPVVDADGMGRAYPEVQMKTYFVYGVPSYPMAVCDEKGNTAL
                     ITEALNAKWVERMARSVTIQMGGVACYALAPMTAYQVQTTAVLDSLSLVKRLGDAVRS
                     ARNTHEDPIEALLNAHPGKVLFQGKISDLDRQTTAGFARGQVVIEGMDQFESDKLTIE
                     FQNENLIARLNSEVICIVPDLICIVDSERGEPITTELLRYGFRVTVLGFPGPDLWKTP
                     EGLATAGPSAFGYDVEYSDLELT"
     gene            complement(2470..3720)
                     /locus_tag="QF838_07705"
     CDS             complement(2470..3720)
                     /locus_tag="QF838_07705"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60601.1"
                     /translation="MEIKSKFFPSLTHKDYKIMWVGHIVGESSSWALGAAEGWLIFNL
                     AESNPSSWVGAVFFAAMIPWFFAPIVVGLLTDKFSRRNILILAYLLSLFHGVALTALI
                     FTGTIEVWHILVLAVVNGIARATHMGAIEALAANLVPANHLPNAYTLISAGYYATRLI
                     GPGLIAPLMELTDIKWIFLSCVIAYAAGVYLVSQVKAVSTGRVDPEKGLLYNTFSGFR
                     YIYRHPVLRSVMYLVMFHCTLVMSFESLLPAVSDNQLASGGEGVAYLHMMIGFGALIA
                     SIALAPVRGHGTLGQLFLISAVVSSFGNIILGVSAVLQFALVSTVVIGISHAAFMTIA
                     TILIQSVSPDDLRGRITSIYLIHAGGLMSFSYFANGVLADMYEPSRILTIGGIAFLIV
                     VIGSWLRQTPRLIYKHGAIIHDSQ"
     gene            complement(3758..4981)
                     /locus_tag="QF838_07710"
     CDS             complement(3758..4981)
                     /locus_tag="QF838_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60602.1"
                     /translation="MEDTKSKFINATLIDALSPEPKEGNISILNGEITEISDNTLPKN
                     SDALTLDLKDKYLVPGLWDVHTHIGKGIPDSEARDETTAERTIRAGNNCRRALDLGIT
                     SLRAVGEKDFIDVAWKEAFASGEHVGPNLYTCGWFITTTAGHFLKSGCAVEVDGPTEY
                     IRTIREQIKNGVDFIKLNLTGGVMGPKWDKMPNTFPLEDELKAAFDICEQRGYKVVAH
                     AGGVDGIKKAISMGAHTLEHGYQFDDEAIDMLAKSQTYYVPTLSLTHMNRGEEFADSE
                     FQLKWMKAHPIDESYKERAIEAAAMHASGFKKAIEAGVEVACGSDLDLPYGTLFETEM
                     LVKGGMTEHQALTAATLTSAKVCLAEEKNGTLEPGKKADFVVLNSNPLENISNLQDVY
                     MVVKDGNIVRDYSSE"
     gene            5170..6333
                     /locus_tag="QF838_07715"
     CDS             5170..6333
                     /locus_tag="QF838_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HJO60603.1"
                     /translation="MKPPRTQLFTPEEYESRIRETREFMAQNQIDVLVIHSPENIYYL
                     SGYQTPGYYWYQALILPLSSDPVFIAPPHEAALVPEFSWIEDSRIYPDTSEWSKVTAE
                     ILKEIGCDSNSIGLETKSRFLSVDFYENLKAILPNSHIVDGSGLIESCRLIKSPRETD
                     YLRKAAEVSSRGMMAGLKAVNEGVTELEVAAEVHTELDLAGSEYTGLPAFITSGERSQ
                     LVHATWSSKRIGIGDLVFMEIPGSINRYHAAQSRSIFVGKPSEKVIEASQVATEALQV
                     AKGSMKEGVSAKTVFKNASSVINESNIDYKQGRRIAYGIGTAFPPGWDEGDIFSINSN
                     EPRPLMAGMCFHLITTMRVPGVGAIGCSDTVLVTKDGVETLTTHVEPGVQYSQ"
     gene            complement(6512..7441)
                     /locus_tag="QF838_07720"
                     /pseudo
     CDS             complement(6512..7441)
                     /locus_tag="QF838_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
BASE COUNT         2086 a         1664 c         1638 g         2223 t
ORIGIN      
        1 acaggaactg ggtgcgcact aattttcact tgatcgacta catcttctac acgtttttga
       61 atttcattga tggtaccttc ggcgaaggag ctttcaatat tggaaacgag tctgaggtcg
      121 cctacttcgg ccattccgtt agcaactgct atgtctgtcg cagtcatttg ccccccacca
      181 aaaaccattg attcactagc cagtttaaaa ccaacgcttt ctggtcctat ggtcaaaggg
      241 ttttgtttta ttaaggatcc gcctccaaga gctgtagaaa aaacatctgg cattcgaaaa
      301 ttagttctga ctcctgcaac atctacagat actgccgcct ctcggggaaa accacggatc
      361 aacattcccc catcggtgga agtacctcca atgtctacta ttactgcatc caatacgcca
      421 gatagaaaag cagcacctcg catactgttg gtcggccctg aagcaatagt gaaaaccggg
      481 taatgagacg caaactccga ttgcattaga gttccgtcat tttgactaaa gaacaaaggc
      541 acatttaaac ctgctgagag catagcggtg cgaattgctg ctaccgtctt tgaagccact
      601 tcaaccagac aagcgttaag catcgctgcg ttttctcgct ctaacaagcc cacgcggcca
      661 atttctgatg acaaagttat gttcacatca ggagtttccg acttaataat ttcggctgcg
      721 atttgctcgt gggagccatc aaccggagaa aatactccac atatggaaat tgaccgcaca
      781 ccctcatcgg tcatttggtt tgctattttc cgtaattctt tctcatcaat tgaagaaatt
      841 tcccgcccgt catattcatg gccacctcca gccatgtatg tatgccctcc tattgaattc
      901 tttaaatcat cgggccaatc gaccagaggg gggagcatcg ttgtcgcggg taaacaaagt
      961 cttaaagctg cggaaggagc taattcgcgg cgttgtaaaa gagcattgac aaaacgagtt
     1021 gtacccaaca ttacggcgtc tatttggtcg acgtctgatc tggagctaat aagggtgttt
     1081 atgcaagaaa ttatgccaga tgttacatca ggagttgttg ggtttttgat cttatccact
     1141 agcacatcac cattcatcaa gacggcgtca gtgtttgttc ccccgacatc aattcctata
     1201 tgcataaatt ttgctaccta ccttgagaga taatttgaat gatcgatgct acacaaagaa
     1261 tctaagtcaa ttctaggtct gaatactcaa catcgtatcc aaaggcagag ggaccggctg
     1321 tagcaagtcc ctctggagtt ttccacagat caggccccgg gaacccaagc actgttactc
     1381 gaaatccgta tcttaggagt tctgttgtga ttggttctcc tctttctgaa tctacgatgc
     1441 atatgaggtc tggcactatg caaataactt ccgaattcaa acgggcgata agattttcat
     1501 tttgaaattc gatcgttaat ttatctgact caaattgatc cataccttca attactacct
     1561 gacctcttgc gaatccggcg gtggtttgcc tgtctaaatc cgagattttt ccttgaaata
     1621 acacttttcc cgggtgggca ttcaacaaag cttcaatggg gtcttcgtgg gtattacgag
     1681 cgctgcgaac agcatctcca agccgcttaa ctaagctcag agaatctaga acagcagtgg
     1741 tttgtacttg ataagcggtc attggagcca aagcatagca agcaactcct cccatctgaa
     1801 ttgttacaga ccgggccatc ctttcaaccc atttggcgtt aagtgcctcg gtaattaagg
     1861 ccgtatttcc tttttcatca catactgcca ttggatagct gggcactcca taaacaaagt
     1921 aggtcttcat ttgaacttca gggtaagctc gtcccatccc atctgcgtca actaccggaa
     1981 ggccagttaa cgcagatggg atgatcggtt ctagagagtt acttccccct atttcgttgg
     2041 agatgatagc ggtagctttc ttgccggtga aatcttcgat ggcttttaat gcataataag
     2101 attgcttgtc ccgtactttc tctattccaa cagtaggagc tccaattccc ccgacacaaa
     2161 cgatattgtc attttcatcg agttcattgg gagatagaac ctttacgggc ccgttttctc
     2221 tgatcatttg tctggcacga agcataccta tgtaaggatt tccacctcca ccagtgccta
     2281 gtatgcctgc cccaattgcg attgattcaa gatcatcttc tgaaatatgc cacatatgat
     2341 ccaccttagg ttaaagatgt aagattttta tagactacca gtcttcggtg tcaatggtca
     2401 cctcaacctg tcgtcatctt ggtctcgagg gttctagata tatgcacggt atttatctca
     2461 agtacctgat cactgtgagt catgaattat ggccccatgt ttgtatatga gcctcggcgt
     2521 ttgtctaagc cagctaccaa tgacaactat aaggaaagcg atacctccga tggtgagtat
     2581 tcttgagggt tcatacatgt cagcaaggac cccgttagca aaatatgaga aactcatgag
     2641 cccgccagcg tgaatgaggt aaatgctggt gattcttcct ctgagatcat ctggtgatac
     2701 agactggatc aggatggttg caatagtcat aaaagcagca tgcgatatac ctatgactac
     2761 cgtacttact aaagcgaatt gtaaaacggc agacacaccc aaaataatgt tgccaaagga
     2821 gctcaccact gcagaaatta ggaacaactg ccctaaagtg ccgtgaccac gtacaggggc
     2881 caaggctatt gatgcaatta gagcgccgaa ccctatcatc atatgaaggt acgcgacccc
     2941 ttccccccct gaggctaatt ggttatcaga tacagctggt agaagagatt caaaagacat
     3001 gacgagagta cagtgaaaca tgacaagata catcacactt cgtaagacag ggtgcctgta
     3061 gatgtaccta aatccagaaa aagtgttgta aagtaaacct ttttctgggt caacacgccc
     3121 tgttgaaacc gctttaacct gacttaccaa atatacccct gcagcgtagg cgataacaca
     3181 ggatagaaat atccatttta tatccgttaa ttccataaga ggagcgataa gtccaggccc
     3241 tatcagtcga gtcgcatagt aacctgctga tatcaacgtg taggcgttag gtagatggtt
     3301 tgcagggaca agattggcag ccagcgcctc tattgctccc atatgggtgg cccgtgcgat
     3361 cccgtttacc accgctaaga cgagaatgtg ccatacctca atcgtacccg tgaatatcag
     3421 ggctgttaga gcaacaccgt gaaaaaggct taagaggtat gcaagaatga gaatgtttcg
     3481 ccgtgaaaat ttgtcagtca acaagccgac tacaattgga gcaaaaaacc aagggatcat
     3541 agctgcaaaa aaaacagccc ctacccaact acttggatta ctctcagcca gattaaaaat
     3601 taaccatcct tccgcagcgc cgagagccca agaggaggac tcccctacaa tatgaccaac
     3661 ccacatgatc ttgtaatctt tgtgggtgag agaagggaag aacttagatt ttatttccat
     3721 gaattaatat ctgagagaat tattattaag ggtgcgttta ttcacttgaa taatcccgta
     3781 cgatattccc gtctttcacg accatgtata cgtcctggag attgcttatg ttttctagcg
     3841 gattgctatt taggacgaca aaatctgctt ttttacctgg ttcgagggtt ccgttttttt
     3901 cctctgctaa acaaactttt gctgatgtta gtgtggcagc ggttaaagct tgatgttccg
     3961 tcatcccacc tttgactaac atttcggttt caaagagggt accgtaaggc agatcaaggt
     4021 ctgaaccgca ggcaacttcc acccctgctt ctatagcttt tttgaagcca gaagcatgca
     4081 ttgctgccgc ttctatggct cgttctttgt agctttcgtc tatgggatgt gccttcatcc
     4141 atttgagctg gaactcagaa tctgcaaatt cttctcctct gttcatatgt gtgagactca
     4201 atgttgggac atagtaagtc tgtgatttag cgagcatgtc aatagcttca tcatcaaatt
     4261 ggtagccgtg ctcaagtgta tgagccccca tagaaatggc tttttttatc ccgtcaacgc
     4321 ctccggcatg tgccactact ttatagccac gctgttcgca aatatcgaaa gcggccttaa
     4381 gttcatcctc gagagggaat gtatttggca ttttgtccca tttgggtccc atcacccctc
     4441 ctgtcagatt cagttttata aaatccactc catttttgat ctgttccctt atcgtgcgga
     4501 tgtattcagt tggaccgtcg acttcaactg cacagccact ttttaggaaa tgtccagctg
     4561 tcgtggttat gaaccagcca catgtgtaca gattagggcc cacatgctct ccagaggcga
     4621 atgcctcttt ccaggccaca tctataaaat ctttttctcc tacggctctc agtgatgtaa
     4681 tcccgaggtc aagggcgcgt ctacaattgt tgccagctct aatagttctc tctgccgtgg
     4741 tttcatctct ggcttccgaa tcaggaatac cttttccaat gtgtgtgtga acatcccata
     4801 agcctgggac taggtattta tcctttaagt ctaaggtcag ggcatcagaa tttttgggta
     4861 aagtattgtc cgatatttcg gttatttcac catttagtat tgagatgttc ccttcctttg
     4921 gttctggaga aagggcatca atcaatgtgg cattaataaa tttagattta gtgtcttcca
     4981 ttctgaaatt catgtctcct attcatttga cccgacttca gtggccaact aatttaatcg
     5041 gactgtatca cgtatatcaa atgtctggct ttgtcatagg ttgagcggat ttagtgacca
     5101 atgctgtaca taagaaggcg caatactgta ttttcaatca tgatccaaat atgttgaaaa
     5161 gataggatta tgaaaccacc aagaacacaa ttattcactc ctgaagaata tgaaagtcgg
     5221 atccgagaga caagagaatt catggcccaa aatcagattg atgttttagt aattcatagt
     5281 ccagaaaaca tatattattt atctggttat cagactccag ggtattactg gtatcaggca
     5341 cttattcttc cactgagttc agatccagtt tttatcgcac cgccgcatga ggcagccctt
     5401 gttccggaat tttcttggat agaggattca cggatatacc cagatacttc ggagtggtct
     5461 aaggtaactg ctgagatttt gaaagaaata ggatgtgatt caaactcaat aggattggaa
     5521 accaaatcgc gttttctatc tgtcgacttt tatgaaaact taaaagctat attaccgaat
     5581 tcccatatag ttgatgggag cggtctcata gaatcttgcc gtttaataaa atctccccgt
     5641 gagactgact atttgagaaa agcggcagaa gtttcttcaa ggggaatgat ggccggttta
     5701 aaggcagtaa atgaaggcgt aaccgaattg gaagtagctg ctgaggttca cactgaacta
     5761 gatttggcgg ggtcggaata tacaggcctc ccggctttta tcacctccgg agaacggtct
     5821 caattagttc atgcaacatg gtcttcaaag cgtataggca taggggattt agtttttatg
     5881 gaaatccctg ggagtataaa taggtatcat gcagcccaat ctagaagcat ttttgtgggc
     5941 aaaccaagcg aaaaagttat tgaagccagt caagtagcaa cagaagcctt gcaggtagcc
     6001 aaaggttcaa tgaaggaagg tgtctctgca aaaacggtgt ttaaaaacgc gagttccgta
     6061 ataaatgaat cgaatattga ctacaaacaa gggaggagga ttgcatacgg tattggaaca
     6121 gcttttcccc caggttggga tgagggtgat atattttcaa ttaattcaaa cgaacccaga
     6181 cctttaatgg cggggatgtg cttccactta ataactacta tgcgggtgcc gggggtagga
     6241 gcaattggtt gcagtgacac agtgctcgta actaaagatg gagtagaaac gctcacgacg
     6301 catgtggaac ctggggtgca atacagccag tagaggttct tcacttcatt aaaaagatcc
     6361 atgatacctt ggtggtaaat ttggaaatcg cttgttaaat actctccctt gcaatctatc
     6421 aattttggtg attggatcag cctggattaa tttttagttt ctgatgccta cggtgccgga
     6481 aatactcaga ataaaataat tccttgaagc tctaatttat tcgatgctcc gtatccatga
     6541 gatgttccat ggtttttatg cactcttctg ttttgtttgt ctgaaaatca aaggtaagat
     6601 ttttgatacc cagattctca cagtgtttta tatcctcgac aacggtgtga gtggagtcga
     6661 tcagggctgc tttagaacgt attcttgaat tggtgtcgat tccaatatct gtgaaatgca
     6721 gtgtcctttt taatgaaatt gttatttcgt ctcgcttgcg gtcatattct tcacactgtt
     6781 gatctataaa ggaaagcata ttttcgatct gatctggccg aagccttgtc ggatgccatg
     6841 tatcgccgta ctttacagtt cttctcaccg ctctctttgt atgaccacca atccatatgg
     6901 ggattccgtt aggtctttga acaggttttg gaaaaaacgc aatattggaa aattcatagg
     6961 tttctccatg aaattctgtg acatcggaag tccacaatag tttaaagatc tgaagatatt
     7021 catcggttgc aagacctcgt tttccaaatg gggcatccaa ggctgcaaat tcttccttcc
     7081 atcaccctac tcctgctcca catataactc ttccttttgt gagtacatca agagaggcaa
     7141 acatctttgc ttgtaatata gggtttcggc aaggaactat tgccactgat gtccccaagt
     7201 ttatttgatt tgtctgtgat gcgatgaaac tcaacaaagt aaatgcatct agttgagggt
     7261 cttgggaatt agcagtgagg cggccgtcgt ccgtgtatgg ataaaggtca gtttcttcta
     7321 tgggtaagac tatatggtct gctgcccata aggaatggaa tccgaggtct tcagctgcag
     7381 cggtgaattt aattaactcg tctggattga tgtgtttggg taatacaatt tcatattcca
     7441 tgcgaaaatc ctttgtttgg aaactacgtg aaaatcgtca ataaattctg tactgggttt
     7501 tcctaggttc aggttatata gaactgagta gatactagtc aaatggattg gtggatatgt
     7561 aattttcata acatatgatg gaagaagtca gagctacatg ataatgttgc t
//
LOCUS       DASCNS010000210         2188 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001612494, whole genome shotgun sequence.
ACCESSION   DASCNS010000210 DASCNS010000000
VERSION     DASCNS010000210.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001612494"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(259..1020)
                     /locus_tag="QF838_07725"
     CDS             complement(259..1020)
                     /locus_tag="QF838_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO60604.1"
                     /translation="MNPMRVRLKAGKTVVGTAGSVFEDNMVMLADSGLDFILFDTQHS
                     PVEPKEYKRSFDAIKGKSATPIMRVSANRPELICFALDLGARGIVVPMVNTKEEATAM
                     VRACQYSPLGDRSNAGVRGEWGDFGDSNYREYLDTVNEELLIIPMIETQQAIDNIDEI
                     LSVPGIDVLLVGPSDLSIELNVPLDYPSDAYQAGLDKIAAACKRHGVVPGMYFIPPTM
                     DPNFYVEKGFKFFTMPWASWALQGIGTGLGAINRE"
     gene            complement(1236..>2188)
                     /locus_tag="QF838_07730"
     CDS             complement(1236..>2188)
                     /locus_tag="QF838_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HJO60605.1"
                     /translation="YKGARLVSLENEFLDSTLWEMAHFDPNYGATDREIWQALMNDET
                     LVVANAIIIPSGDPFGPPDRSFKSSFIEPGDLKAMDSFGIEMKKRRSSLDPTQLTVIG
                     VIERLASGTGFGGGSATFYSPHALGSKIAQEDVPLDTFYFSLKDKNEASNYSQKLEKI
                     FLANGMNAVSLIDQLEKERATSDAFTKLFQGFSGLGLVVGVAAIGVLSVRAVVERRQS
                     IGVLRAIGFRSSMIRTQFLIESSFITLLGIFVGICLGILQSWLIFLEISKELEGAKFS
                     VPIGEVGVLIGITIIASILPSVIPANEASKTYPAEALRYE"
BASE COUNT          635 a          502 c          420 g          631 t
ORIGIN      
        1 cttacatcca caacattcat atctttttat taatccagct ggtcgaactg gtttaaaaat
       61 aaccagcaat atggaactca aaatcgttct ggaaaaggaa caatactgaa gtctgtaatc
      121 ttaaaaacgg ctaattccgg gggttaaatt atttatatag gtggtttacc caaaaaaagc
      181 cgtcgcagga agttcactag tcctgcaacg actcaattcc cttgccaaac tgccctttta
      241 aaggcagcag atttgggttt attctcggtt tatcgcacca agcccagtcc ctatcccttg
      301 caaggcccaa gatgcccatg gcattgtgaa aaatttgaac cctttttcta cgtaaaaatt
      361 aggatccatt gtcggaggta tgaaatacat gccaggtacg acaccatgcc ttttgcaggc
      421 tgccgcaatt ttgtctaatc ctgcctgata agcatcactt gggtaatcca gtggaacatt
      481 aagttcaatt gacaaatctg atggcccaac caaaaggaca tctatccctg ggacgctgag
      541 gatctcatct atattatcta ttgcctgttg agtctcgatc atgggaataa tcaagagctc
      601 ttcatttact gtatctaaat attccctgta gttagagtca ccgaaatcgc cccattcacc
      661 acggacaccg gcattactgc gatctccaag tggtgaatac tggcaggctc gtaccattgc
      721 cgttgcttcc tctttagtat tgaccatggg cacgactata cctctcgcac caaggtctaa
      781 ggcaaaacaa atcaattcag gtcgatttgc gctgactctc attatgggtg tggcagattt
      841 acccttaatc gcgtcgaagg atctcttgta ctccttgggt tcaacagggg aatgctgagt
      901 atcgaaaagt atgaaatcta aacctgaatc ggccaacata accatgttgt cctcaaaaac
      961 agatccagcc gttccgacta ctgttttgcc cgcttttaat ctaactcgca ttgggttcat
     1021 ttatatctcc ttttacttcc agatttattt aatgcctagg attcatataa ccaaatttgg
     1081 atacctatcc atcagggcag gggtgattct atcactaacg attggtagtg tgaggagaaa
     1141 gcctttttca aggggcaccg acaaattgct ccctgaatta ttgagccaac acaatgtcaa
     1201 caaggattct tttgtgaagt gtaatggtga atcggctatt cgtatcgtag cgcttcggca
     1261 ggataggttt tactggcttc attggcgggt ataacgctgg gcaaaattga agctatgata
     1321 gtgatgccaa taagtactcc aacttcccca atagggacac taaacttagc tccttcgagc
     1381 tccttcgaaa tttccaggaa aattagccat gactgaagta tgccaaggca aattccaacg
     1441 aatattccca atagggttat gaatgaggat tcaattaaaa attgggttcg aatcatactt
     1501 gatctaaacc ctattgcacg aagaactcct atagattgcc ttcgctccac cactgctctt
     1561 actgatagaa caccaattgc agccacaccc acaactaatc ccaaaccact aaaaccttgg
     1621 aaaagtttgg tgaatgcgtc actggtagca cgttctttct ccagttgatc tataagactc
     1681 accgcattca taccatttgc taaaaagatt ttttctaatt tttgagagta gtttgaagct
     1741 tcatttttgt ctttaagaga aaagtaaaat gtatctaaag gaacatcttc ttgagcaatt
     1801 ttggaaccca aggcatgagg agaataaaaa gtggcgctcc cacctccaaa tccagttcct
     1861 gaagccaagc gctcgatcac acctataact gttagttgag tgggatctaa tgaactcctt
     1921 ctttttttca tttctatgcc aaaagaatcc atcgctttta aatcacctgg ttcgataaat
     1981 gaactcttga agcttctgtc aggaggtcca aaaggatctc ctgatggaat tattatcgca
     2041 ttagcgacta ccagagtttc atcattcatt aaggcctgcc atatctccct gtcggtggcc
     2101 ccataattag ggtcaaaatg ggccatttcc cacagggttg agtcaagaaa ttcattctct
     2161 aaggaaacaa gacgagcccc cttatatg
//
LOCUS       DASCNS010000211         7459 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001613294, whole genome shotgun sequence.
ACCESSION   DASCNS010000211 DASCNS010000000
VERSION     DASCNS010000211.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001613294"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(292..1512)
                     /locus_tag="QF838_07735"
     CDS             complement(292..1512)
                     /locus_tag="QF838_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60606.1"
                     /translation="MISSLRKIHYSWVVLAAVCGLAFSNSVASLNVLTVFFLPMSDEF
                     LWNRTQISGAASLGALLGAGVAVVTGRILDQTGPRVILAIGALITTISMIALSQISAI
                     IAFYIFYGAARVSDQGFIQAVSPSIIAKWFDRNSGMAVSVLFAVTALGGVLLPLISQA
                     SIEEFGWRFGWLVLGLLMFLVGALPVILFVRSPTDLHENDSDEVESGNFTFPEFSLSE
                     VLHTKEYWFLTTTVFATGIATAGIGLHIVPFLVGQGLNPEEAVATVSVRFIASAIGGL
                     IGGVLADRYTPRLLILTAISVRAFSIALLIFADTFFKAVFSGALGGISDGIQSTVLVL
                     LLVSYFGKNNVGGIYGVNRAAAVLGFSAGPMIAGIGFDVTGSYNYIFCGFLLLTILSI
                     GLVFSANRTFPAVR"
     gene            complement(1650..2969)
                     /locus_tag="QF838_07740"
     CDS             complement(1650..2969)
                     /locus_tag="QF838_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60607.1"
                     /translation="MPDDGETPKFTSALEEKRQISPNFTIGSKKLASAIVLGHAVSHV
                     FNSSLPLLLAMMKTDPRFNLTATQFGVVGGFGRGASDATTMVAGYLGERFSDRPGAMM
                     AISLTIMGTSFFLLGMAPSFWWLLGAMLLVGIGPSLYHPPAIAELSRRFPDRRGFAIS
                     LHGTGGSIGEMFGPFIVGLLTTETFKVGSIITMTYLIAFEWNEVLRISVLPALIFALV
                     VYIMMRGVSKDVPKAESFAEYFGGLRELLKDKGIWGLILVTGLSDMGQSSIMYFLPLY
                     LITAVGEGGLAKDPLTVGVFLAGAQVAGIIGQPVLGLLSDKCGRKIVLLPSMTILGIS
                     FIGLYYASDGYQLVLNIFVMGAFLYSLHTVFISAAMDVSGGKSQPTVVSLIYGASLLG
                     TVSPIFTGMLIDEFGIKSAFLFSGIICLIATVVLLWVKLPMNVGMAD"
     gene            3097..4236
                     /locus_tag="QF838_07745"
     CDS             3097..4236
                     /locus_tag="QF838_07745"
                     /inference="COORDINATES: protein motif:HMM:NF037020.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HJO60608.1"
                     /translation="MAQIVGNLVLITMLVAMLACSNNISDNSIANPQIAPKLLPIEFG
                     TSGPWINSEPFVLQEKRNEVVLIDFWTSTCINCIRTLPHLNEWHRKYNEKGLSVLGVH
                     SPEFEFEKIYGNVVSAAEYLGIDYPIVMDNDFNIWNTFDNRYWPSKYLLDKDGVIQFS
                     HFGEGGYEETELAIRALLEEAGFDTSDIPLEVNLHQKGDSSNISNEQTLELYMGTDRN
                     YKFNFFQDTYIGNKEYYENIRTDAWKKKDVFKSPSKKQKNRVYIEGLWSKEKEYISHS
                     RNTDAYIDQIFLKFSGAEVNVVLGKHKTPYKVLITLDGFPVKKSEAGKDVYFDPSGKS
                     FVQVDSSRMYNFVKLEKFDTRELILSSTSSGFSIYSFTFGSDVRE"
     gene            4245..5174
                     /locus_tag="QF838_07750"
     CDS             4245..5174
                     /locus_tag="QF838_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60609.1"
                     /translation="MSYNRFLSIFLFCIGITTLTTSCNDIKDPFTDEYPIKSSITEGG
                     YQIVLATTDLAVGENRFAFIVLSETGFINRDYSMVTFYAPIKHSQESKKTAQFMHWDD
                     LNRGSFVANVNFPYPGKWTFQVDLQDNEKDISVQANFTVNEKTVAPNEGDKAPITKNK
                     TLDSVVNIQQLSTGNVIAPDLYRHSIKDAMENGKATVVTFSSPAFCQDETCGPQVKVL
                     QKLSSYMNSSINFIHVEIYDNPVELQNDSKAGLYSETFKKWNLPSFQWTFIINCLGEI
                     SHRYQGFVSSEELDGAIKFFIPGKGGKNHCLVI"
     gene            5356..6036
                     /locus_tag="QF838_07755"
     CDS             5356..6036
                     /locus_tag="QF838_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016874.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HJO60610.1"
                     /translation="MNAKVNTEYTIYFAGALFDHKELIGNAILASYINKLSKEKYRCI
                     LPQDLEQSTNRAVDIRNQDLNCVLSCDLAIFNFDGTELDSGTVVEFLYAKTLDIPALI
                     IRSDFRGSGDQNREGDQWNLMASFYPRTRKLEFNAMEWYQREIKASSEDQNIFDTADR
                     LYKKFASSVISELDIVIKDPPLLSTDKEQAFSLYQWASVFPGNGLPEIVPNIEDIIIS
                     KKNKGLIN"
     gene            complement(6071..6949)
                     /locus_tag="QF838_07760"
     CDS             complement(6071..6949)
                     /locus_tag="QF838_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014035.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HJO60611.1"
                     /translation="MEQTFSDLMALTLGDIGLPSDHLVWKALLLTIISLLVGILGGFV
                     GLALGTIRLPALLLLGTPAAIAGGTNIMVSSLSSPSGAIKHWRGGRVDMRIVIVMGIP
                     AFAGAFAGGFLSGVIHEGILLFLAGLLVLWQGVEFIIIYQNRVKSGQSTSPLFGAGLE
                     GSSGLFTPGRVSAEAGIGLGVGLLGGAVGLILGSIRLPALVRVLKIDPRIAAGSNLFI
                     GFFMGAVGWIGHASQGKVDYPVLILMGAGAMVGSWYGAKLTGKVNLNTLILTMGFVLL
                     AVGLLLACRGALPLFQ"
     gene            7110..>7459
                     /locus_tag="QF838_07765"
     CDS             7110..>7459
                     /locus_tag="QF838_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016852.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HJO60612.1"
                     /translation="MRSMHMADAGKVLLEIRQLLNDTNIIFFLRHGTCLGAVRDGELI
                     PWDDDIDIGSIIGMYNLDEPSILQAVDEFKRAGFQVKVLETDFHIGVELSKLGIPVDW
                     TCYRVLEKSILQYPG"
BASE COUNT         2415 a         1648 c         1437 g         1959 t
ORIGIN      
        1 attaacctcc cagattaagt tccctacaaa acgggaacct tcggcccttt ttcctcagga
       61 ttttgatgtt taatttgatt gattctgaac ttctatatcc tgcccgtaat tggttacgag
      121 tacatgaaag gtatcagcct agacatcacc atccatgttt atgacgtcag ccagtttcaa
      181 tcggaaaaca taatatgaaa accggattcc acatgcctct agaatcattc aatggtattt
      241 ctaggcctaa agtgacattg caaggatagt actgcagtca caccttcaca tttatcgcac
      301 ggctgggaat gtccgattgg cactaaagac cagacctata ctaagaattg tcaaaagtag
      361 gaacccgcaa aaaatgtagt tatagctccc tgttacatcg aaccctatac cggcgatcat
      421 tgggccagct gaaaaaccaa gaactgccgc cgccctattg actccatata ttccaccgac
      481 attattcttt ccaaaatagc tcactaatag caaaactagt actgtggatt gaattccatc
      541 tgaaatccct ccaagagccc cggaaaacac agccttgaaa aaagtatcag caaatataag
      601 taacgcgatc gaaaatgccc ttacagaaat ggccgtcaga attagtagtc gaggtgtata
      661 gcgatcagcc aaaacaccac ctattaatcc cccaattgct gaagcaataa accgaacgct
      721 aactgtagca accgcttcct ctggattcaa accttgacct acaaggaagg gcactatgtg
      781 tagccctatg cctgcagtag caatgccagt ggcaaatact gttgtcgtta gaaaccaata
      841 ttccttagtg tggagaacct ccgacaaaga aaattcaggg aatgtgaagt ttcccgactc
      901 aacttcatca ctatcattct cgtgaagatc tgtaggagag cgaacaaata aaataaccgg
      961 aagtgctcct actagaaaca ttaataaacc caaaaccaac catccaaatc gccaaccaaa
     1021 ttcttcaatc gatgcttggg atatcaaagg aagtaataca ccacctaaag ccgtgacagc
     1081 aaataaaaca gatacagcca ttccactatt tctatcaaac catttagcta tgatagaagg
     1141 ggaaacggct tgaataaatc cttgatcact tactcgtgct gccccataaa aaatataaaa
     1201 cgctatgatg gcggagattt ggcttaaagc aatcatagaa atagttgtaa ttaaggctcc
     1261 aattgccaat atgacccttg gtcctgtttg atccaggatt cttcccgtca ctactgccac
     1321 ccctgccccc agcaaggcac ccaggcttgc cgccccggaa atttgtgtac ggttccacag
     1381 aaattcgtcg gacatcggca aaaagaagac ggttaaaaca ttaagactag ccacagagtt
     1441 agagaaggcc aaaccacaaa cagctgccaa cacaacccag ctgtagtgaa ttttcctcaa
     1501 tgaactaatc attaaccata ggttcgcctt gccctgtgat attgaatttt gagtccccat
     1561 tataactagt cattgaaggg gatttctaag catttcatct ccgatcaatg cttggaaact
     1621 catttcccca aaaactaagg tgtgtttttt cagtcagcca tgcctacatt cataggaagc
     1681 ttgacccata agagaaccac ggtggctatt aaacaaatga tcccgctaaa tagaaaagca
     1741 gattttatcc caaattcatc gatgagcatt ccggtgaaaa ttggggaaac tgttcccaat
     1801 aaacttgccc cataaatcaa cgagactact gttggctgtg attttccacc cgaaacatcc
     1861 atagcagcgg aaataaaaac agtatgcaat gagtataaga aagcacccat aacgaatata
     1921 ttcaacacaa gttggtatcc atcagatgcg taatacaatc cgataaatga aattcctaat
     1981 atagtcatac taggcaatag aactattttt cttccacact tgtcagacag caaaccaagt
     2041 acaggttggc caataattcc agctacctgt gctccagcca gaaaaactcc cactgtaagt
     2101 gggtctttgg caagccctcc ctctcctaca gctgttatca ggtaaagggg aagaaaatac
     2161 ataatcgaag attggcccat atcgctcaac cctgtgacca gtattaagcc ccaaatccct
     2221 ttgtctttta gcaattctct cagaccgccg aaatattctg caaaagattc tgccttaggg
     2281 acatccttcg aaacacctct catcataata taaacaacta gagcgaaaat taaagcaggc
     2341 aaaacactta ttcgaagaac ttcattccat tcaaatgcga ttaggtatgt catggtaatg
     2401 atgctaccga ccttgaatgt ctccgtagtt aacaagccca ctatgaaagg tccaaacatt
     2461 tctcctattg aaccacctgt cccgtgaagg gatatcgcaa atcctcgtct atccgggaat
     2521 cttcttgata gctctgcaat cgctggtggg tgatacaaag aagggcctat tccgacaagg
     2581 agcatcgccc caagaagcca ccaaaaacta ggagccattc ccaacaaaaa gaaggaagtc
     2641 cccataatag ttaacgatat tgccatcatg gccccagggc ggtcagagaa acgttctcca
     2701 agataacctg caaccattgt ggtagcatcc gaggctcctc gcccgaatcc tccaacaaca
     2761 ccaaattgag tcgctgttaa attgaaacgt gggtcagttt tcatcattgc aagcagtaag
     2821 ggaagcgaag aattaaaaac atggctcact gcatgcccta acacaattgc tgaggccagt
     2881 ttcttacttc ctatagtaaa gttaggtgaa atctgtcttt tttcttccaa tgcacttgta
     2941 aatttgggag tctcaccgtc atccggcatt acaaaaccct tgaagatatc ttttttattc
     3001 tgaagccaag cgttactgtc tcaatgcagt ttttaatgtc ctttttcata tgtgcattat
     3061 agctattaac aagtatgttt tagaatgaga attaatatgg cacagatcgt tggaaatcta
     3121 gttttaataa ccatgctcgt ggcgatgctc gcctgctcta ataacatctc agacaattca
     3181 atagcaaacc cccaaatagc cccgaaactt ctccctattg aatttggcac ttcagggcca
     3241 tggatcaata gtgaaccatt tgtcttacag gaaaagagga acgaggtcgt tctaatcgat
     3301 ttttggacct ccacctgcat caactgcata aggactcttc cgcacctaaa tgaatggcac
     3361 cggaaataca acgaaaaggg tctaagtgtt ttgggagtgc atagtcccga atttgagttt
     3421 gagaaaatat atggcaatgt cgtctctgct gcagagtatt taggaattga ttatccgata
     3481 gtgatggata acgatttcaa tatatggaac acatttgata atcgatactg gccttcgaaa
     3541 taccttttgg ataaagatgg ggtgatccaa tttagccact ttggtgaagg gggatacgaa
     3601 gagacagaat tagcaataag agctttatta gaggaggctg gcttcgatac ctcagatatc
     3661 cctttagaag ttaatctcca ccaaaaaggt gattcgtcca atatttccaa tgagcaaacg
     3721 ttggagttat acatgggtac tgatagaaac tacaaattca acttttttca agatacatat
     3781 atcggaaata aagaatacta tgaaaatatt cgaaccgatg cttggaagaa aaaagatgtc
     3841 tttaaaagtc cctccaaaaa acaaaaaaat agggtttata ttgaaggttt gtggagtaaa
     3901 gaaaaagaat atatttctca cagcagaaat actgatgctt acatcgatca aatttttcta
     3961 aagttctcag gggctgaggt gaacgtggtt ttgggaaaac ataaaacccc ctataaggta
     4021 ctaatcaccc tggatggatt ccctgtaaag aaatcagaag cgggtaagga tgtctacttt
     4081 gaccctagcg gaaagagctt tgttcaagtc gacagttctc ggatgtataa cttcgttaag
     4141 ttggaaaaat tcgacacgcg agagctaata ttaagctcta catcatctgg gttctccatt
     4201 tattctttca ctttcggctc cgatgtaagg gaataatcat ttaaatgagc tacaacagat
     4261 tcctttctat tttcttgttt tgtattggca taaccacctt aacgacctct tgcaatgata
     4321 ttaaagaccc atttacagat gaatatccga taaaaagctc aatcaccgag ggaggctatc
     4381 aaatagtttt ggctacgaca gatctggccg taggggaaaa tcgattcgca tttatagttc
     4441 tgtctgagac agggtttatc aatagagatt attccatggt gaccttttat gctccgatca
     4501 agcattcaca agaatcaaaa aaaactgccc agttcatgca ttgggacgat ctcaacagag
     4561 gatcttttgt cgccaatgtg aattttccgt accctggaaa atggacattc caggtagatt
     4621 tacaggacaa cgaaaaagac atttcggttc aagcgaattt cactgttaac gaaaaaactg
     4681 tcgccccaaa tgaaggtgat aaggctccca taacaaagaa caaaacctta gacagcgtag
     4741 taaatatcca gcaactctca acaggaaatg taattgcccc tgacctgtat cgacactcaa
     4801 tcaaggatgc aatggaaaat ggtaaggcca ctgttgttac tttttccagc cccgctttct
     4861 gtcaagatga gacatgtgga ccacaggtga aagtgctaca aaaattaagc tcttatatga
     4921 attcttcaat aaatttcatc catgttgaaa tttatgataa cccagtcgaa ctccaaaatg
     4981 attctaaggc tggtttgtac tctgagactt tcaaaaaatg gaacttgccg agctttcaat
     5041 ggacattcat catcaattgc ctcggtgaga tatcacatcg gtatcaagga ttcgtatcct
     5101 ctgaagaact agacggagcc ataaagtttt ttatcccagg gaaaggaggt aagaatcatt
     5161 gtttagtaat ctaatttgag aaaattacat tcgtttaata taacttaatt cagagggccc
     5221 tacccccaaa taaaataaga aatctcagaa gcctcaaatg aaccccctta tactgactgg
     5281 ttagtttcca tctgtatttt gcctaaagtt tgacttgggt tggtttacaa tcctcatcac
     5341 aagggatgaa agattatgaa tgcaaaagta aataccgaat acacaatata ttttgccgga
     5401 gcgttattcg accacaagga acttatcggt aacgctattt tagcctccta cataaataaa
     5461 ctttccaagg agaaataccg gtgcatacta ccgcaagacc ttgaacaatc tacaaatagg
     5521 gcagtcgaca taagaaatca agatttaaat tgtgtgttgt catgtgactt agcaattttt
     5581 aattttgatg gcacagaatt agactcaggg actgtcgtag aatttttgta tgctaaaact
     5641 ttggacatac ctgcattaat cataaggtct gattttcgag ggagcggtga tcaaaatcgt
     5701 gagggagatc aatggaattt aatggcatcc ttttatcctc ggactagaaa gctagaattt
     5761 aatgcaatgg agtggtatca aagagagata aaggcttcat ctgaagacca aaacatattc
     5821 gacaccgctg atcgcttata taaaaagttt gctagttcag ttataagtga attggatatt
     5881 gtgataaagg acccaccctt gctctctacc gataaagagc aagcttttag cctttatcaa
     5941 tgggcctcag tgtttcctgg aaatgggctc ccagaaattg tcccgaacat agaagacata
     6001 attatatcca agaagaataa ggggttaata aattagaata aacccgtacc cttttaatta
     6061 agataagcaa ttattgaaac aatggtaagg cccctcgaca tgctaacaac aacccgacag
     6121 ctagtagaac gaagcccatc gtaagaataa gagtgttcag attcactttc ccggttaact
     6181 tcgcaccata ccaactacca accatagcac ccgctcccat aaggattaaa acagggtaat
     6241 cgacctttcc ctgactagcg tgccctatcc atcctacagc tcccataaaa aatccgataa
     6301 aaagattaga tcctgcagca atacggggat cgattttaag aactctgaca agagctggta
     6361 agcgtatgct gcccaaaata agcccaacgg caccgcccag aagcccaacc cccaacccaa
     6421 ttccggcttc tgcagaaacc cgcccaggag tgaatagccc tgaagatccc tctaatccag
     6481 ccccgaatag aggagaagtt gattggccgg attttacacg gttttggtag ataatgatga
     6541 attctactcc ctgccacaac acgagtagtc ctgcgagaaa taggagtatc ccttcgtgta
     6601 ttacaccact gaggaatcct ccagcaaagg cccctgcaaa agcaggaata cccatcacta
     6661 ttactatcct catgtccacc ctacctcccc gccaatgctt tattgcgcct gacggagaac
     6721 taagactgct gaccataata ttagttcctc ccgcaatggc agctggtgtc cctaaaagca
     6781 gcagtgcagg taagcgaatc gttcccaaag caaggccaac aaatccacca agtattccta
     6841 ctaatagaga tataatggtc agtaagagag ccttccagac aagatggtcg gacggaagtc
     6901 caatatctcc aagggtcaaa gccattaaat ctgagaaagt ctgttccata tagtctctct
     6961 ttccacgatt atgcgctttc agaaagccaa tgaataggaa tggtacaact gttactagtt
     7021 caaatgtatg ataccaaggc acttatccca agaaaccgga tacgtaatgt cacgtatggc
     7081 atcgcttcca gccaattagg agagagaaaa tgagatcaat gcatatggca gatgcaggga
     7141 aggtactatt ggagatcaga cagttgctaa atgataccaa tattattttt tttctgcgcc
     7201 acggaacctg tctaggtgct gtcagggacg gggagcttat accttgggat gacgatatag
     7261 atattggttc aattattggc atgtataatc tcgatgagcc gtccatttta caagccgtag
     7321 atgagtttaa acgggcaggc tttcaagtaa aagtattgga aacagatttc catatcggtg
     7381 tagaactttc aaaacttggc atacctgtcg attggacctg ttacagggta ttggaaaaaa
     7441 gtattttgca gtaccctgg
//
LOCUS       DASCNS010000212         3904 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001624368, whole genome shotgun sequence.
ACCESSION   DASCNS010000212 DASCNS010000000
VERSION     DASCNS010000212.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001624368"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="QF838_07770"
     CDS             <1..848
                     /locus_tag="QF838_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60613.1"
                     /translation="NLLMCSMPGFGLTGPYSEYPAFGSTAEAMAGINSMIGYVPNRPL
                     QTGLSYADPVSGLNLVHAILAFVRRRAVTGKGSFIDLALADSPLGTLGEFFVANSATG
                     YEQHPNGNRHSEYAPHGAFRCFGNDQWIAISITNDFQWEQLKDEVNDIRLESPDYDDI
                     SGRKVNEEAINRIVEEWTSRKDAGDLMKQLQQKRVPAAKVSNNLDVLNDPHLNRREYF
                     SHFRISEENIEKYDGQAIPGNQKERSKWFPMRDLGEDSNTILVEYLGYSDDKYLSLLE
                     QEVIS"
     gene            863..1537
                     /gene="queC"
                     /locus_tag="QF838_07775"
     CDS             863..1537
                     /gene="queC"
                     /locus_tag="QF838_07775"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HJO60614.1"
                     /translation="MPNYSHVLVVSGGMDSATLAHMCVKEGGSPYLITFDYGQKHKKE
                     IEYARLLAENLGVPHQVVDISNIQNLLGGSALTDDGIEVPEGHYAASNMAITVVPNRN
                     AIFLTIAYGAAVSFQSKFVGAAMHTGDHTIYPDCRPQFVDIFSTMQKIATEGTGDADL
                     SLWTPFIDKSKAEIAQIGLNMDLNYEDTWSCYKGDLLHCGKCGTCVERKEAFEIIGAT
                     DPTLYQ"
     gene            1665..3443
                     /locus_tag="QF838_07780"
     CDS             1665..3443
                     /locus_tag="QF838_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60615.1"
                     /translation="MNRRTWVSATATRNYAINDPLLDWLHYHGKSKGFNPDTEYEDYD
                     ERTDFRLFIMNQGNRFESSVTEYISKLFPIHRVREPMESSSDDSVFSKTLSAMRSGSP
                     IINQAVLRDENTETYGIADFLIRSDVFGELFSRYREDKSTRLASPLLGDESWHYRVLD
                     AKFTTLRFSAAGNLSTSGSAWAYMLQLFIYNRALGNMQGYTPPNAYLLGRKWSQTARG
                     ETLRGTNFMDRLGEVPMDYFSVSRGTLGDAVSNACEWIRNVRTIGHSWEVLPNPSIPE
                     LRPNMSSTADQPWHHAKGEINDSLKDLTTLWGVGVEKRNLANKEGIFKWNHDGLKAEE
                     LGVKAKGSAKTLQAILDVNRIETYPVEPSFIEDLDNTWRQPAKVEFFVDFETVSDLND
                     DFSNTPESGGTPLIFMIGCGHLEAEKWIWRSFTTERLTEEHEGLIIDQWMDYMYQVQN
                     HLDPGGYSPTVYHWSHAEVSTFDSAFNSAKNRHSDKEWPSLNWYDFLKEVIKKEPVVV
                     NGAFGFGLKAIAGSLKTLGLIETSWESGPTDGLGAMVGAWWADGQAEQHGLSMTQIPM
                     VQEISEYNEVDCKVMMEIIQYLRKNH"
     gene            3520..>3904
                     /locus_tag="QF838_07785"
     CDS             3520..>3904
                     /locus_tag="QF838_07785"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJO60616.1"
                     /translation="MSTQTAFRPPVMGMTHMVSAGHYLAASAGYRILEDGGNAVDAGV
                     ASGIVINVTLPNATNFGGVAPIMVYMAEKNETKTISGLGRWPKKTSLDDFNNRLNGDI
                     PKGIDRTIVPSAPDAWLTALAEYGTM"
BASE COUNT         1175 a          786 c          892 g         1051 t
ORIGIN      
        1 caaatctact gatgtgttca atgcctggat ttgggctcac agggccttat tcagaatatc
       61 ctgcatttgg atcaactgcg gaggcgatgg ctggaataaa ttcaatgatt ggttatgtcc
      121 caaacaggcc tttacagacg ggtctttctt atgctgaccc ggtttccggt ttaaatctcg
      181 tgcatgcgat cctggccttt gttcgtagga gagctgtaac aggtaaggga agtttcatag
      241 atttagctct ggcagatagt ccattaggga cacttggcga gttttttgtt gcaaattctg
      301 ccactggcta tgagcaacac ccaaatggta accggcattc agaatatgcc ccccacgggg
      361 ctttcagatg ttttggaaat gatcaatgga tagccatatc aatcacaaac gattttcaat
      421 gggaacaatt aaaagatgag gtcaatgata tacgattaga aagccctgac tatgatgata
      481 tttcaggtag gaaagttaat gaagaagcca taaatagaat agtggaggaa tggacttcaa
      541 ggaaagatgc cggtgactta atgaaacaat tgcaacagaa aagggttcca gcggctaaag
      601 tgtcaaacaa tctagatgtc ctaaatgatc cccacctcaa tcgaagagag tatttttcac
      661 actttagaat ttcagaggag aacattgaga aatatgatgg gcaggcaata cctggcaatc
      721 agaaggaaag atctaaatgg tttcctatga gggatcttgg ggaagattcc aataccattc
      781 tagtggaata ccttggttat tcggacgata aatacctatc tctgttggag caagaggtca
      841 tctcctaaag gaagttttgt aaatgcctaa ttattcacat gttcttgtcg taagtggagg
      901 tatggactct gctaccctgg cccatatgtg tgtgaaagaa ggggggtcac cttatctgat
      961 cactttcgac tacggtcaaa aacacaaaaa agaaattgaa tacgcgcgat tactcgcaga
     1021 aaatttgggt gtcccccacc aggttgtcga tatatcaaat atacaaaatc tactcggagg
     1081 ttcggccctt accgatgatg gtatagaggt accagaaggc cactacgcag cctctaatat
     1141 ggcaataaca gtagtgccaa accgaaacgc aattttccta acaatagcat acggcgcagc
     1201 tgtttccttc caatctaaat ttgtaggggc agctatgcat actggtgacc atactatcta
     1261 ccccgattgc cgtcctcaat ttgtagacat ttttagtacc atgcaaaaaa ttgccacgga
     1321 aggaacgggt gacgcagacc tcagtctttg gactcctttc attgacaaaa gcaaagcaga
     1381 aatagcccaa atcggtttaa atatggacct aaattatgaa gacacttgga gctgttacaa
     1441 aggtgatctc ctgcattgtg ggaaatgcgg tacatgcgta gagcggaaag aagcttttga
     1501 aattattgga gctaccgatc caaccctata ccaatagaac aagttagttt cttataaatt
     1561 gaatatccga cacgaaacca atgacttttg ccgatttttt atttcagcgg tttatactac
     1621 ctagaatatc gaatggcttt gaatcaatta aggaaactca ttttttgaat cgtcgaacat
     1681 gggttagtgc aacagcaaca cgcaattacg cgattaacga tcctctttta gattggcttc
     1741 actatcacgg taagtcaaaa ggttttaacc cggacactga atacgaggat tacgacgaaa
     1801 ggactgattt taggcttttc atcatgaatc aaggaaatcg gttcgaatcc tccgttacgg
     1861 aatacatttc taaactattc cctatacata gagtacggga accgatggaa tccagttccg
     1921 atgacagtgt gttctccaaa actttatccg ctatgcggtc aggttcccca attattaacc
     1981 aggctgtcct tagggatgag aacaccgaga cttatgggat cgctgatttt cttataagaa
     2041 gtgatgtttt tggggaacta ttttctcgat atagagaaga taaatcaacc agattagcat
     2101 cccctctctt aggggatgag tcatggcatt accgtgttct cgatgccaaa ttcaccactc
     2161 tcagattttc agcagccggc aatttatcga ctagtgggtc tgcctgggcc tatatgttgc
     2221 aacttttcat atacaacagg gccttgggta atatgcaggg atacacccct ccaaatgcat
     2281 atttactcgg tcgaaaatgg tcacaaacgg ccagaggcga gacgcttcga ggaaccaatt
     2341 ttatggatcg tcttggtgaa gtacctatgg attatttttc tgtgagcaga ggaacccttg
     2401 gagatgccgt atctaatgcc tgtgaatgga tacgaaatgt gcgaactatt ggccattctt
     2461 gggaagtgct gcctaaccca tccatccctg aattgaggcc aaatatgagt tccacagcgg
     2521 atcaaccctg gcaccacgca aaaggtgaga taaacgactc tttgaaagat ttgaccaccc
     2581 tttggggagt cggggtggaa aagagaaacc tggcaaataa ggaaggtatc ttcaaatgga
     2641 atcatgatgg tttaaaggct gaagagctgg gtgtcaaggc gaaaggaagt gcaaagaccc
     2701 tgcaagcaat cttagacgtg aacagaattg aaacttaccc tgtcgagcct tcatttattg
     2761 aagatctaga taacacctgg cgccagcccg caaaggttga atttttcgtt gactttgaaa
     2821 cagtttccga tcttaatgat gatttttcaa ataccccaga aagtggtggt actcctttaa
     2881 tatttatgat tggctgtggg catttggaag cagaaaaatg gatatggcgc tcttttacaa
     2941 ctgaacgtct aaccgaagaa catgaggggc tcatcataga tcagtggatg gattatatgt
     3001 accaagtgca aaaccatctt gaccccggtg gttacagccc tactgtttac cattggtccc
     3061 atgccgaagt ttcaactttt gactccgcct tcaattcagc aaaaaatcgt catagtgaca
     3121 aagaatggcc atccttaaat tggtatgatt ttttaaaaga agtgataaaa aaagaaccag
     3181 tagttgtaaa cggtgccttt gggtttgggc ttaaggccat agcagggtct ttaaagacct
     3241 tgggattaat agaaacctct tgggagtctg gaccaaccga tggtctaggg gcaatggttg
     3301 gagcctggtg ggccgacggc caagccgaac aacatgggct ctccatgacc caaataccta
     3361 tggttcagga aatttccgaa tataacgagg tagactgtaa agtgatgatg gaaataatcc
     3421 aatatttgag aaagaaccac taaatatttc ataggatttg ggacatacag gatttccttg
     3481 taatataggc tcgaagcatt aagccaagcg gagattgaga tgtctactca gacagcattt
     3541 agacccccag taatgggaat gacacacatg gtttctgccg gacattattt ggcagcctca
     3601 gctggttatc gaatattaga agatggtggt aacgcagtag atgccggagt cgcatcaggg
     3661 atagtcatta atgtgactct acctaatgcc accaactttg gtggggtagc tccaataatg
     3721 gtctacatgg ccgaaaaaaa cgagaccaaa actataagtg gactcggtcg ttggcccaag
     3781 aaaacctctc tggatgactt taataatcgc ctaaatggtg acatacctaa aggtattgat
     3841 agaactattg ttccttcggc tcctgatgct tggcttacag ctctggcaga gtatggaact
     3901 atgt
//
LOCUS       DASCNS010000213         8156 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001635956, whole genome shotgun sequence.
ACCESSION   DASCNS010000213 DASCNS010000000
VERSION     DASCNS010000213.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001635956"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="QF838_07790"
     CDS             <1..342
                     /locus_tag="QF838_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60617.1"
                     /translation="LADRGTTGGYTKIATVITADRSKLAQLLPGSSIRFEIVGQTKAV
                     ELLREQETLLASFLNKEGSPLKFKVDGMLVDAVDDSGLPLSVQNPETKTYAAKVSHEA
                     GEHQVEVEISE"
     gene            360..1412
                     /locus_tag="QF838_07795"
     CDS             360..1412
                     /locus_tag="QF838_07795"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HJO60618.1"
                     /translation="MGKKMAVVGAGAAGSYIGAFLTREGHDIVLIDMWGEHVEKMNSN
                     GIDVSGSQGPFNVPVQAYHFYDASQLKEKYDIIFFAMKSYDTIWAAHFAKNLLSPNGV
                     LVSNQNCMNDLTLASIVGFEREIGCVMSGITVGLWEPGHVNRGGQPGKDRGHDVFRVG
                     ELHGRITPRAEEIAELLSCIDGSRATSNIWGERWSKLTTNASSNPITAMTGLGSNGAA
                     ELPEARRLQIEISKESCRVALAQNFDVEPIKGITADVWSRANEGDVYEELDEKFLPNP
                     GASDWKSSMSQDVTKGRKTEVTLMNGYISEQGALAGIPTPVNTAITSVVDEIDRGVRE
                     ADVVNIKEVLARAGIT"
     gene            1419..2045
                     /locus_tag="QF838_07800"
     CDS             1419..2045
                     /locus_tag="QF838_07800"
                     /inference="COORDINATES: protein motif:HMM:NF020893.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HJO60619.1"
                     /translation="MFEILHELSNWIREFAQTDWAVLILFVTAFLESTISPIPPDPLL
                     IPMGIINPSAAIWFAALCTLASVLGAVLGHWLGSRFGRPLLGSFVSESRIQSGESLFD
                     KYGIWAVLVAAVTPIPYKVFTILAGVLKLDMKRFLIASIIGRGMRFLSLGIVIFLFGE
                     DVQHFLEEQFEALTLLAGVGVVVSVLFVVILAKWKIRRGSREKQSDVE"
     gene            complement(2047..3090)
                     /gene="ruvB"
                     /locus_tag="QF838_07805"
     CDS             complement(2047..3090)
                     /gene="ruvB"
                     /locus_tag="QF838_07805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HJO60620.1"
                     /translation="MKHVEDRLVSGSTHEEDHNLDTNLRPKNLSSYVGQDGIKNTLSI
                     SITAAKQRGEPLDHLLLYGPPGLGKTTLSNIVATEMEVRIKTTSGPAIERAGDMVAIL
                     SQLQQDDVLFIDEIHRLSRVVEEVLYPAMEDFFVTWVIDKGLKARSMNLSLKPFTLIG
                     ATTRYGMISSPLRDRFGSIHRLDFYTVQDMEQILLRSARILDVAYSPDGLHEIAKRAR
                     GTPRVANRLLRRVRDYAQVVADNIITQDVALKALADLKIDNLGLDGVDVNVVKCIIEK
                     FDGGPVGIDTIAASINEEAETIEDVYEPYLIQMGFLDRTQRGRVATRRAYEHLGYEFN
                     QPSPSQLQSRMEL"
     gene            3266..4918
                     /locus_tag="QF838_07810"
     CDS             3266..4918
                     /locus_tag="QF838_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HJO60621.1"
                     /translation="MAKRTIEEWEENTLTPLKERFGERKDSFETTSGIPINTIYTEQT
                     KPGPTELLDEYPGEYPFTRGVQPNMYRGRLWTMRQYAGLASPEESNRRYRYLLDQGQT
                     GLSVAFDLPTQTGYDSDHPMATGEVGRTGVPISSLADMEILFKDIPLDKVSTSMTINA
                     TASVLLALYIAVGKKQGVIPEALSGTIQNDILKEYIARGTYIYPPEPSMRLITDVFQF
                     CSTNVPKWNTISISGYHMSEAGANAVQELAFTFSNAIAYVEAAISSGLDVDAFAGRLS
                     FFFVAQSNLFEEVAKYRAARRMWAKIMRERFNAKNDRSCMMRFHTQTAGVTLTAQQPD
                     NNVIRTTIQALAAVLGGTQSLHVNSKDEALALPTEESVQISLRTQQILAHESGVSESV
                     DPLAGSYYVEHLTDELESQAFQYIESMDKMGGAVEALREGFQIQEIHNSAYQHQKEVE
                     SLDRVVVGVNQYQSPTPPIEKLQTISPKETQNQLARLAKVKSERNAKEVKESLAKLRT
                     VTEGGSNTMPALIKCVESYATVGEISDVFRDIFGEQTEFTPF"
     gene            4940..6091
                     /locus_tag="QF838_07815"
     CDS             4940..6091
                     /locus_tag="QF838_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60622.1"
                     /translation="MSDLLLSEDEILLKNTVSDYSKNELAPRAAGYDESALFPWDNVS
                     GIGDLGLFGLTIPEEYGGSGGTTRQLAIAAEEIAKGCAATSVVYIAHLSLCTQFINMF
                     GSEEQKRNFLPDLASGKKIGAFALSEPGAGSDAAAIKTSAVRSNGHYVVNGVKTWISN
                     APEASIFVTLATSDTSKKHKGIEALIVESDTEGIVTNQLHGKMGVRASTVGEIIFDNA
                     LVPAKNRMGGDEQGFKQTMEVLNASRISIGAQCVGIAQAALEAAVDYALHREAFGGII
                     SDLQGIQWMIAEMATEIEAARQLVMRSASLRDANLPFATEAAMSKLYGSKVAMESAHR
                     AVQIHGGTGYFAPTPVERYFRDARVTEIYEGTSEIQKLVIARNILKKQG"
     gene            6096..6530
                     /gene="mce"
                     /locus_tag="QF838_07820"
     CDS             6096..6530
                     /gene="mce"
                     /locus_tag="QF838_07820"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HJO60623.1"
                     /translation="MNNESTCSARHINHVCIAVLDIQETLEFYENLFGVTRSGEIETI
                     EDQGVKAALVRVGASQLEFIQPIDNENGVAKFIDRKGEGVHHICFEVDNLESKLKDLD
                     LAGVQLIDKEPRDGLSGTIGFIHPKATRGVLIELVDQKTARR"
     gene            6532..6927
                     /locus_tag="QF838_07825"
     CDS             6532..6927
                     /locus_tag="QF838_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HJO60624.1"
                     /translation="MTSKIIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGIRQT
                     PEMIAQAAMQEDVDVIGLSILSGAHIELFPQIISELNNREMGDTLIVAGGIIPESDHN
                     YLTDIGIKGIFGPGTSTSDIVDFIHESVK"
     gene            7035..7622
                     /locus_tag="QF838_07830"
     CDS             7035..7622
                     /locus_tag="QF838_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014690.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HJO60625.1"
                     /translation="MTLILACLVSYIFGALPFAHRISRRKGIDIFSIGTGLAGASNVL
                     KNVGRWSALIVLVFDITKGSLAVIVSSMMGVEGPLILLPACAAIFGHWNSIFTRFRGG
                     DGMAIAGGIAIGLFGGIGFFALFVAGIASLLAQKLWVTSLTSIIFGYLAILFLTHLLD
                     KNKDIAVGFTAVVLLILAHAMRGHARRRRIARQSE"
     gene            complement(7632..>8156)
                     /locus_tag="QF838_07835"
     CDS             complement(7632..>8156)
                     /locus_tag="QF838_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60626.1"
                     /translation="CYVRKSGKNEVYSIPCSQNGIFSSDPDSWRNPIVISIPPSDVTS
                     FDYIYPDSNENFSIYKTQENDWVVVNPDGILEGPASLQIMDYLLQSVQVLPATGFEND
                     QTAKSLDFDAPDGAVRINTSEESNSPTTRLKLIKKDEEFYYIKTPSQSTVYLIQYILG
                     DFLLMGKSDILVSD"
BASE COUNT         2387 a         1535 c         1948 g         2286 t
ORIGIN      
        1 cttgctgata gaggtacgac tgggggatac accaagatag cgaccgtgat tactgcagat
       61 aggagtaaat tggcacaatt actaccagga tccagcatca ggtttgaaat agttggccaa
      121 acaaaagctg tggaattatt gagagagcaa gaaacattgc tagcctcatt ccttaacaag
      181 gaaggctcgc ctttaaaatt taaagtagat ggtatgctgg ttgatgctgt tgatgactct
      241 gggctgccat tgtctgttca aaatcccgaa acgaagacct atgctgctaa ggtgtcacat
      301 gaggccggtg agcatcaagt ggaagtagaa atatctgaat agaattatga ggtaaacgca
      361 tgggtaaaaa aatggctgtt gtaggggctg gagcggccgg tagttatatt ggagcttttc
      421 ttaccagaga aggacacgat attgttctta tagacatgtg gggggagcat gtagagaaaa
      481 tgaattccaa tggaattgat gtttcagggt ctcaaggacc tttcaatgta cctgtacaag
      541 cgtatcattt ttatgatgct tcccagctta aagagaagta cgacattata ttctttgcca
      601 tgaaatctta tgacactatt tgggctgctc atttcgctaa aaatttacta tcaccaaatg
      661 gagtacttgt aagtaatcag aattgtatga atgatttaac tcttgcttca atagtaggtt
      721 tcgaaaggga aattggttgc gttatgtcgg ggataacagt ggggctttgg gaaccaggcc
      781 atgtaaatcg tggcggacag ccaggtaagg accgcgggca cgatgtgttc agggttggag
      841 aactacatgg caggattact ccgagggcag aggagattgc ggaacttttg tcttgtattg
      901 atggctcaag agcaacctca aacatatggg gtgagagatg gtcaaaactc actaccaatg
      961 cctcctcaaa ccctataacg gctatgacgg gactgggttc aaacggtgca gcggaacttc
     1021 cagaggccag aaggttgcaa atcgagattt caaaggaatc ctgccgagta gctcttgctc
     1081 aaaatttcga tgttgaacca ataaaaggca tcactgcaga tgtttggtca cgtgcgaatg
     1141 aaggtgatgt ttacgaagaa ttggatgaaa aatttttgcc aaatccagga gctagtgatt
     1201 ggaaatcttc tatgtcacaa gatgtaacca aaggtcgtaa aaccgaagtt accctgatga
     1261 atggatatat ttcagaacaa ggtgccttgg ctgggatacc cacccctgtt aacacagcta
     1321 taacctccgt agtggatgaa attgaccgag gtgtcaggga agcagacgtg gtaaacataa
     1381 aagaagtttt agctagagct ggaattacat aaacaagtat gtttgagatt cttcacgagt
     1441 tatccaattg gatcagagaa tttgcccaaa cagattgggc agttctaata ctgtttgtca
     1501 cagcattctt ggaatctact atttctccta ttccccctga tccattactt attcctatgg
     1561 gaataattaa ccccagcgcg gccatatggt ttgccgcttt gtgcacattg gcttctgtgt
     1621 tgggagccgt attgggacac tggcttggat ctcggtttgg gagaccacta cttgggagtt
     1681 ttgtctctga aagtagaatt caatcaggag aatccttgtt cgataaatat ggtatttggg
     1741 ctgtgttggt ggccgctgtt acccccatac cttataaagt gttcaccata ctggccggtg
     1801 tgctgaaact agatatgaaa cgattcctta tcgcatctat cattggacga ggaatgaggt
     1861 tcctcagcct ggggattgtg atatttctat tcggtgaaga tgtccagcat tttttggaag
     1921 aacaatttga ggctctgacc ttgttagcag gtgtgggtgt cgtggtttct gttctttttg
     1981 tggtgatact tgctaaatgg aaaattagaa gaggttcgag agagaaacaa tcggatgtcg
     2041 agtagatcaa agttccatac gggattgaag ttgactgggc gaaggctgat tgaactcata
     2101 tcctaaatgc tcataggccc ttctggtggc tactcggccc cgttgagtac gatctaggaa
     2161 tcccatttga atcaaatatg gttcgtacac atcttcaata gtttctgcct cttcattaat
     2221 tgaagcagct attgtgtcta ttcctaccgg ccctccatca aatttttcaa taatgcattt
     2281 cactacgttc acatccactc cgtcgaggcc caaattatct atttttaaat cagctaaagc
     2341 tttaagtgct acgtcttgag ttattatgtt gtccgctaca acttgtgcat aatccctcac
     2401 ccgcctcaac aacctgtttg cgacccttgg ggttccccta gctcgtttcg caatttcatg
     2461 aaggccatct gggctgtagg ctacatcaag aattcttgct gaccgaagaa gaatttgttc
     2521 catgtcctgc actgtataaa aatccagtct atgaatggat ccgaacctgt ctctaagggg
     2581 tgagctaatc ataccgtaac gagtagtcgc gccaattagg gtaaaaggtt ttagggaaag
     2641 attcatgctt ctcgccttga gtcccttatc aataacccag gtcacaaaaa aatcttccat
     2701 agccggatac aaaacttcct ctacgactcg gcttaaacgg tgtatttcat ctataaaaag
     2761 gacatcatct tgctggagtt gactcaatat agctaccatg tctccagctc tttctatggc
     2821 tggaccggat gtggtcttta ttctaacttc catttccgtg gctacgatat ttgacagggt
     2881 tgttttgccg agtccaggag ggccataaag cagaaggtgg tctaaaggtt cacctctctg
     2941 tttggctgca gttattgata ttgaaagtgt gttttttata ccatcttgac ctacataaga
     3001 agataaattt ttaggacgta aatttgtgtc aagattatga tcttcttcgt gagtactgcc
     3061 agaaacgagt ctatcttcca cgtgtttgat agtcctttgt taaagtttta cttttcccag
     3121 attctaggtt tgatataaac tcagtgtcaa ttaaaactta aagcggttac aacattacac
     3181 cgtacaggtg ggtttgacac gtttttttat aacccaataa actcacaatg gaaagattac
     3241 ctaattgtgt tggagaatct gttttatggc aaaaagaaca atcgaagaat gggaagagaa
     3301 tacattaact cccttaaaag agagattcgg tgagagaaag gattcctttg aaaccacatc
     3361 ggggataccg ataaacacca tctataccga acaaaccaaa ccggggccga ccgaactttt
     3421 ggatgaatat cctggggaat atccctttac gcgtggcgtc cagcccaata tgtacagagg
     3481 acgtctctgg acgatgaggc aatatgcggg actagcttct ccagaggaat cgaatcgaag
     3541 atatcgctac ctactagatc aaggtcaaac ggggttaagt gtagcgtttg atttgccaac
     3601 tcaaacggga tatgactcag atcacccaat ggcaacaggt gaggtaggtc gtacgggtgt
     3661 tccaatatcc agcttggctg atatggaaat tttattcaag gatattccgt tggataaagt
     3721 aagtacatct atgaccataa acgcaactgc ttcagttctg ctggccttgt acatcgccgt
     3781 tggcaagaaa cagggggtaa taccagaggc attaagcggg acaatacaaa acgatattct
     3841 caaggaatat atagcgagag gcacctacat ttatcctcca gaaccatcaa tgcgtttgat
     3901 caccgatgtt tttcagtttt gcagtacgaa tgttccgaaa tggaacacga taagcatcag
     3961 tggctatcat atgagtgaag ctggtgccaa cgcagtgcaa gaattagcct ttactttttc
     4021 gaacgcgatt gcttatgtag aggcggctat atcctcaggg ttggacgttg acgcatttgc
     4081 aggacgcctt tcttttttct ttgttgcgca aagtaatttg tttgaggagg tcgccaaata
     4141 tcgagcggct agaaggatgt gggcaaaaat aatgagagaa aggtttaacg caaaaaatga
     4201 tcgctcttgt atgatgcggt ttcatacaca aacagcaggt gtcaccttga ctgcgcaaca
     4261 gcctgataac aatgtgataa gaacgacgat ccaagccttg gcagcagtat taggcgggac
     4321 gcaatccttg catgtaaatt ccaaggatga agccttagcc ttacccacgg aagaatcagt
     4381 acaaatatct ctcagaaccc agcaaatact ggcccatgaa agcggagtat ctgaatctgt
     4441 ggatccactg gcaggttcct attatgttga gcatcttact gatgaattgg aatcgcaagc
     4501 ctttcagtat attgaatcta tggataagat gggaggagct gtagaagcac taagggaagg
     4561 atttcagata caggaaattc ataacagtgc ctaccaacac cagaaagaag tggaaagcct
     4621 agatagggtg gtggttggag ttaatcaata tcaatcacca actcccccaa ttgaaaagtt
     4681 gcaaacaata tcccctaagg aaactcaaaa ccaactggca agattggcca aggtgaaatc
     4741 tgaacgaaac gccaaggaag taaaagaatc cttagcgaaa ttgaggaccg tgactgaagg
     4801 tggatcaaat acgatgccag cattaataaa atgtgtggaa tcatatgcca ccgtgggaga
     4861 aatatcagat gtgttcaggg atatttttgg agaacaaaca gaattcaccc cattttaatg
     4921 gactgagagg agtaaaaaga tgtctgatct gcttttgagt gaggatgaaa ttttattaaa
     4981 aaacacagtc tctgattatt ccaaaaatga attggcccct agggctgcgg gatacgatga
     5041 aagcgcactg tttccttggg ataacgtatc tgggataggt gatttgggtt tattcggcct
     5101 aacgatacca gaagaatatg gagggagtgg tggcacgacg aggcaattgg caatagcagc
     5161 agaagaaatc gccaagggct gcgcagccac ctcggtagtt tacatagctc acctatcttt
     5221 atgtacccaa tttataaaca tgtttggtag tgaagaacaa aaacgtaact ttctgccgga
     5281 tcttgcatcc ggtaaaaaaa tcggggcttt tgctttgtca gaacctggag ccgggagcga
     5341 tgcagcagct ataaaaactt cggccgttag gtctaatgga cattatgtcg tgaatggtgt
     5401 aaagacttgg attagtaacg ctcccgaagc ctctatattt gtgactttgg caacatcaga
     5461 cacatctaaa aaacataagg gaatagaagc tctaatagtg gaatcggata ccgagggaat
     5521 agtgacaaac caactccatg ggaaaatggg ggtcagggct tcgactgtgg gggaaatcat
     5581 ttttgataac gccctggtgc cggcaaaaaa caggatgggt ggagatgaac aagggtttaa
     5641 gcaaacaatg gaagttttaa atgcaagtag aatttccatt ggagcccaat gtgttggcat
     5701 agcacaagca gctttggaag cagcagttga ttatgctttg catcgagagg ctttcggggg
     5761 gatcatttca gatttacaag ggatccaatg gatgattgca gagatggcta ccgaaattga
     5821 ggcagcgaga cagttggtga tgagatctgc gtctcttaga gacgcgaacc ttccattcgc
     5881 cactgaagcc gccatgtcaa aactgtatgg atctaaagtt gcaatggaga gtgctcacag
     5941 ggctgttcaa atacacggcg gtacaggata cttcgctcca acacctgttg aaaggtactt
     6001 tagggatgcg agggtcacag aaatatatga agggacttct gaaattcaaa aattagttat
     6061 tgcaagaaac attttaaaaa aacaaggata atttggtgaa taacgaatct acatgtagcg
     6121 ccaggcacat aaatcatgtg tgcattgctg ttttggatat tcaggagact ctcgaatttt
     6181 atgagaattt gtttggggtt actcggtccg gggaaataga aactattgag gatcagggtg
     6241 tcaaagcagc cctggtgagg gtcggtgctt ctcaattgga atttattcaa cctatcgata
     6301 atgaaaatgg agttgctaaa ttcatagata gaaaagggga aggagttcat catatctgtt
     6361 tcgaagtaga taatttggaa tccaaactca aagacctaga tctagcggga gtccagttga
     6421 tagataaaga accacgggat ggtttatcgg gaaccatcgg atttatacac ccaaaggcta
     6481 ctagaggcgt tttaatagaa ttagttgatc agaaaacggc tcggagatga tatgacatcc
     6541 aaaattatta gagtgctggt tgctaagcca gggttagatg gccacgatag aggtgccaaa
     6601 gttatagcgc gggcgcttcg cgacgcgggt atggaggtga tttataccgg tatcaggcaa
     6661 accccggaaa tgatagctca agcggcaatg caagaagatg tagacgtaat tggcctcagc
     6721 atattgtcag gcgcgcatat tgaactcttc ccgcagatca ttagtgaatt gaataatagg
     6781 gaaatggggg ataccttgat tgttgcaggt gggataatac ctgagtcgga ccataactat
     6841 cttactgata taggcataaa aggaatattt ggcccgggta catcaacttc agatatagtt
     6901 gacttcatac acgaatcggt caagtaattt tcgccgggat aattcaaatt aaatctatca
     6961 atgctagttt tatagaatta agttagtatt aacttcagat ttttcagata aaaatgttat
     7021 aaatggaaga atatttgaca ctcatattag cctgcctggt tagttatata tttggggctc
     7081 tcccctttgc ccacagaatc agtagaagaa aaggaataga tatattttct attggaactg
     7141 ggctagcagg ggcctctaat gttcttaaga atgtaggtcg ttggtccgct ttaatagttc
     7201 ttgtatttga tataacaaag ggttctttgg cggtcatcgt atcaagcatg atgggggtgg
     7261 aggggcctct gattttactt ccagcatgtg cggcaatatt tggtcattgg aattctatat
     7321 tcactcgctt caggggaggg gatgggatgg ccattgcagg aggtattgct atcggtctct
     7381 ttggcggaat aggttttttt gctttgttcg tagcaggtat agcttcccta ctagcccaga
     7441 aactttgggt gacctcttta acaagtataa ttttcggtta cttggctatt ttgttcctta
     7501 cgcatctctt ggacaaaaat aaggatattg ctgttggatt taccgcggtt gtattgttga
     7561 ttttggctca cgcaatgaga ggccacgcac ggagaaggag aattgctaga cagtccgaat
     7621 gaactggctg cttagtcaga tactaaaata tcggatttcc ccatcaataa aaaatcacct
     7681 agtatgtatt gaattaaata tacagtagat tggctcggtg tttttatata atagaactct
     7741 tcgtctttct ttatcaattt caaccgtgtt gtcggtgaat tagattcttc tgatgtgttt
     7801 atccgtactg cgccatcagg agcgtcgaaa tcgagtgatt ttgcggtctg gtcattctcg
     7861 aatcctgttg caggtagaac ctggactgac tgtaataaat aatccattat ttgaaggctc
     7921 gcagggcctt cgaggattcc atctgggttc acgaccaccc aatcattttc ttgggtttta
     7981 tatatagaaa aattctcatt gctgtctggg tatatatagt caaaagacgt tacatctgac
     8041 ggaggtattg atattacaat aggatttctc caagaatcgg ggtccgagga aaaaatccca
     8101 ttttgagagc aaggtatcga atacacttca tttttcccag attttctcac gtagca
//
LOCUS       DASCNS010000214        17853 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001647170, whole genome shotgun sequence.
ACCESSION   DASCNS010000214 DASCNS010000000
VERSION     DASCNS010000214.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 17853)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17853)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17853
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001647170"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(165..806)
                     /locus_tag="QF838_07840"
     CDS             complement(165..806)
                     /locus_tag="QF838_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HJO60627.1"
                     /translation="MELTSWVQIIFICFLGAITPGPSLAVVVTNTINRGKICGSVTGI
                     SHAFGIVIWAMLTVVGLSTIILNYEVVLNIFQIIGACLILYIGYRTSITASKSVPSPK
                     TLETLAPNRKLTRAALEGFGISIMNPKIALFFLAIFSQFVNTGSNWVEIFIMGLTAGI
                     IDGAWYTTVALVIGNNKMVSTISKRRSLILRASGILLALISIYLLIDAARNII"
     gene            complement(1606..1866)
                     /locus_tag="QF838_07845"
     CDS             complement(1606..1866)
                     /locus_tag="QF838_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60628.1"
                     /translation="MKIIMARPRLRLMIPMVAACLIGAIAFRCGSKDASTGVAQLDDN
                     NPIIANIANSENVPETETARENLPESTPSTVDISDSRNSQQI"
     gene            2112..3101
                     /locus_tag="QF838_07850"
     CDS             2112..3101
                     /locus_tag="QF838_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HJO60629.1"
                     /translation="MPEGKELYMLMLRRMMLIRKFDETVKDLVQSAELVGMAHCYIGE
                     EAVAVGACTALRDEDYITGNHRSHGHPISKGGDVRRAMAELLGKATGYCKGKGGSMHL
                     ADFDIGILGESGIVGSALPVAVGAAMGSKMQNNDRVVISFFGDGASNQGACHEAMNLA
                     SIWKLPVIFLCENNQYAVTTSFKDTVSTENVSDRATAYNMPGVLVDGQDVMAMHEATV
                     HAIQRARAGEGPTLLEAKTYRYEDHSEGLNRILREPYRTEEEVEFWRQRDPIDIHSKW
                     LLENDVASQEEIDKVQHDVYEAIGDALQFARESPYPDEADLFTDMFADPIPLR"
     gene            3152..4117
                     /locus_tag="QF838_07855"
     CDS             3152..4117
                     /locus_tag="QF838_07855"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HJO60630.1"
                     /translation="MGEITYSDALNHALKEEMRRDEKVFLMGESIRGGIYGVTGGLSQ
                     EFDKRVIDTPLSENGFMGAAVGAAAVGMRPIVQSLASFAWVAMDQLVSQAAKMRFMFG
                     GQVNLPIVYRWGLLYGNNIAAHHADRPYPMFMNMPGLKIAIPSTPADAKGLLKTAIRD
                     NDPVMFFEDNNLAGSRGEVSEDPEICIPFGKAEVKREGADVTVVTIAGMLRPALAVAD
                     ELESDGISVEVIDPRTIVPLDTKTILDSVEKTGRLVVVDPAHRSCSVASEISSIVSQE
                     GFWSLQAPIQRVTSLDCHFPFSPALEHLVYPNEDKIADAINLTLE"
     gene            4162..5529
                     /locus_tag="QF838_07860"
     CDS             4162..5529
                     /locus_tag="QF838_07860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HJO60631.1"
                     /translation="MAINVALPQWGMNMEEGILVKWLVDQGEEIRKGQPLVEVETSKI
                     SSELESPADGVIAHLMAPEGAVVKVGDLVAVIGEPGENPPRPEKTASKPGRRKSTQES
                     RFSLNKPNRIKRTQATPIARKLAKDNNIDIDELTGSGPNGRISEGDVREYIKRDKAGT
                     RTKTGQISPRARRLALNNNIDLNQISGTGPNRRISVADVENAIGSIADAGVDEKSIGA
                     SQTLSGLRKTISERMSLSVRTMAQVTLTSEIDATKMVGVRKNLLQSWRSHKIRPMDQD
                     ILVAAVGDALTKHPRLNSFLKNDTVLLAKDINVGIAMAVPDGLMVPVVNSVNETSLIE
                     IAKNLRDLANKTRKNSLNIAEVSNAGFTITSLMNHDIDVFTPLVDPPQVAILGIGRIT
                     DKPVVVDDEIVIRQIMHISVTFDHRALDGVPVAEFIHTLKSNLEDPEWLKERCLNNQL
                     PSRRN"
     gene            5704..6483
                     /locus_tag="QF838_07865"
     CDS             5704..6483
                     /locus_tag="QF838_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014376.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJO60632.1"
                     /translation="MLPISTEPMQIRTSNIIDSKGDGPWYEALFSDGRNNVGLICDTL
                     GSVNDDHYHPDFNEFWIILKGSLEFEIGDFPTIHAHKGDVVLAPAGERHLIRTIGNES
                     SVRLHVSKIGSDHANRKDRSNQTVPYPTQKEPPNLLHTTLDSVIEQLGEPQWVTSIIK
                     DSLNTANLVYSAPGTPHDAHWHPEFDEWWTILKGELTWNLGEKRPVYQVKEGDIMYLP
                     RGLKHHILTQGSETSFRLAITDSEGLHVFQEGDEDAPPPRE"
     gene            complement(6782..7963)
                     /locus_tag="QF838_07870"
     CDS             complement(6782..7963)
                     /locus_tag="QF838_07870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60633.1"
                     /translation="MSLPDRLGFGIFLAPFHAPFENPTLALERDLELLEWLDYLGYDE
                     AWIGEHHSCGWETISSPEIFIATAAQRTKHIKLGTGVIPLPYHHPLIVANRMVLLDHL
                     TRGRTAMGVGPGALNSDGHMLGLPHEQARPRMEESLDVIMRLLTEEEPFTYRSDWFEL
                     VDAHLQLRPYSQPHFEIMVASAQSPIGMKLAGKHGATPVSLTFARSPGGFYHNTLKDL
                     WEIGVQSAAEFGREMNRENWGLVQHVFLADSKKEAMSIARRNSGLLMREYFDKVVGNP
                     PIDGPADQIIDKMVDEGIWCIGTPDDLVDSINRLDEESGGFGKFIFLATELGTHEQMK
                     NSYELAARYVMPKFQGSTASLDMSFDFFRGMRDTLAEERESALHNASRAYEAERANWK
                     K"
     gene            complement(8396..8548)
                     /locus_tag="QF838_07875"
     CDS             complement(8396..8548)
                     /locus_tag="QF838_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60634.1"
                     /translation="MKVILALGGRPEHRAVYVVDTDEVDKLYEFLRPALSWTKCDIAP
                     VRELDF"
     gene            complement(8693..9313)
                     /locus_tag="QF838_07880"
     CDS             complement(8693..9313)
                     /locus_tag="QF838_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60635.1"
                     /translation="MPEFIQNVSRIPKQGKNFDVTEGSIDLIKGLNQNGNVSFTISTP
                     PALKSRVISALTFDTLGEIEAFHDGFLENDQSRDKFQKLAADCEDVNVSIIRNVRQPE
                     VFSPEAKYMIRTIFVAKRGEAPNLLDTVLEMADSSKTMDPLISVPMFGNPDVVRIVNG
                     INSLEDVNSIIAEVNGESFAPFRSKVSSLTVRMFRTLSRIAYRTGS"
     gene            complement(9698..10075)
                     /locus_tag="QF838_07885"
     CDS             complement(9698..10075)
                     /locus_tag="QF838_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HJO60636.1"
                     /translation="MYTERISVTDKAKEVIDQLREKHGDLMFHQSGGCCDGSAPMCFK
                     KGEFIVGSIDLSLGTIHGCEFFMAEDQFEYYKYSHITIDICQGRGSSFSLEIPLGLRF
                     MTVSRILTNDEYKSLRPPEHKYR"
     gene            complement(10120..11613)
                     /locus_tag="QF838_07890"
     CDS             complement(10120..11613)
                     /locus_tag="QF838_07890"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJO60637.1"
                     /translation="MSNRPEFKSQYENYIGGEWVPPVDGDYFDNNSPVDGSLIAKVPK
                     SNKNDIDLAVKAGWKAAPEWNSSSATERSALLNKIADRVEENLDYLAKAETWDNGKAI
                     RETTAADLPLTIDHFRYFAGVIRAESGEMSDLDSNTVSMEIHEPLGVVGQIIPWNFPI
                     LMAAWKLAPALAAGNCVVLKPAEQTPVSILLLMELIEDLIPKGVVNIVNGFGPEAGQP
                     LAKHPDIKKIAFTGETTTGRLIMQYASENIIPFTLELGGKSPNIFFDSVMAEDDNFLD
                     KAIEGLVLFAFNQGEVCTCPSRALIQENIYDAFMERSLKRIEAITMGDTLDGGTMMGA
                     QASNDQYQKILNYIDIGKQEGAEVLIGGEAYQNETYPDGYYIKPTVFKGNNKMRIFQE
                     EIFGPVLSVTTFKDEAEALEIANDTLYGLGAGVWTRDIHQMQSMSRGIEAGRVWCNCF
                     HAYPAHASFGGYKKSGIGRETHKMMLDHYRHTKNILISYDKNPLGFF"
     gene            12222..13157
                     /locus_tag="QF838_07895"
     CDS             12222..13157
                     /locus_tag="QF838_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014615.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HJO60638.1"
                     /translation="MKKLQERIAQGEVIIIDGPMGTELERRGVPMHGEAWSAAALYTH
                     PEVVRQAHEDYIRAGADIIITNTFASARHTLERTEMADQVRELNTRAVTLAKEAREAT
                     ASDRPVYIAGSISTSFRGGTQEASANYREQAEILAEAGADFLIMEMMVDVQQSAYAIE
                     AAVSIGIPVWVGFSCKIRDDDSQVVFRELPWTHGKDEDKTFAEALDSLLPIGGSLVSV
                     MHSDIEATAPALRAVQERWDGPLGAYPESGRFVNFSWQFENVIPPEDFLSEAKKWVQM
                     GTQVVGGCCGIGPEHIRLLREGLPSHVPAEQQSVS"
     gene            complement(13168..13243)
                     /locus_tag="QF838_07900"
     tRNA            complement(13168..13243)
                     /locus_tag="QF838_07900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13207..13209),aa:Arg,seq:cct)
     gene            13386..14312
                     /locus_tag="QF838_07905"
     CDS             13386..14312
                     /locus_tag="QF838_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013530.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60639.1"
                     /translation="MSQKIEKVLITGATGYVADQMLAFMKEQYETVLVDTREVNRRGE
                     PIEGVNIVDLIDPHRNKYSDLFEGVDAVIHLAYKRRSGDPLDHFNDEKQNVEMAYNVF
                     RSSYDAGVQRVVMASSNHAADWYEHALIHNGGLDIVGPYDLPLSDNFYGWAKATYEHM
                     GFLFASGGMGEGGDGASGSAATGNLLAGNLNTSRKMGVVMVRIGAPRDIETEIYRGKA
                     AAYKRDLGAYISPRDITQLFHRAMETPNIENEYGVPWQVVYGISNNTRAFWSLTSARK
                     VLGYEPEDDSEITFRAGINEILNDSGRVGPLN"
     gene            complement(14354..15337)
                     /locus_tag="QF838_07910"
     CDS             complement(14354..15337)
                     /locus_tag="QF838_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60640.1"
                     /translation="MGKLKVSYLPHPIISVEERHQYLPEILLKEFDTEIFDRSKDWPS
                     QLTGREVIVDLGGNIEPELVDVAAEAGIKYVQVQTNGLDHVEVERIIEKGMILAHCPG
                     HLSSVSLAEGAMMFTLMLAHDYGNATDQFFAGTVFSANGLEVEGRTLTIVGFGASGQQ
                     LARRAKAFGMKINAIDVRPIEQEILDEIQPDFLGGTEDLDDAIGDCDFLSVHLHLTEE
                     TKHIINRKRIALLKPTSFVINVARGGLIDEDALYDALRDKKIAGAGLDAFAKEPPEFD
                     LPVYKLPNVIVQPHTVAGTDGMMRKRADFAVENLKRYSDGKELEGQILKRL"
     gene            15510..16358
                     /locus_tag="QF838_07915"
     CDS             15510..16358
                     /locus_tag="QF838_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60641.1"
                     /translation="MPESTLTIDCQVHAYEKDSLSRPWKGFLQGPDEVTGDDMVAAMD
                     SVGVDGAILISPASLYAYDASYALEVYAKHPGKFGLVKPFDPESETVGEEVEEWAATP
                     GVVGARIMLRPYEFTEYHPGLSSILDAGAEAGIPINIMCSGNLDLFSQLADKHPNTQM
                     VIDHLGIPQPHLPPVPENPFADLDKVISLAKHDNVVIKISGAGTLSHESFPYADIWGP
                     LGKIFNAFGLDRCLWGTDWTRAVELLTYKEGVEAFRVTDSLSDSEREILMGGSLSKIY
                     KWSPKK"
     gene            complement(16383..16742)
                     /locus_tag="QF838_07920"
     CDS             complement(16383..16742)
                     /locus_tag="QF838_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60642.1"
                     /translation="MSFIEVESWEIKQGHQDDHDEIIRQWFLFVEKYHDKLFPEWKSA
                     KYFRQLDSSGNPTGRYIMNFEFHSLEGHDSYKERRKNWDGPYEEYKSVDPYEHFIIES
                     VTVEYWEPQEQDSWLSF"
     gene            16810..17805
                     /locus_tag="QF838_07925"
     CDS             16810..17805
                     /locus_tag="QF838_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60643.1"
                     /translation="MVQFSIAISGDREIKEYISIAKIVDQSDFSNLSIYDDLMFKPAW
                     PILSIIAINTERIKIGPAVLNPYLTHPAVIAANISVIDEISNGRSFVGIGRGAFLDFL
                     NIKSEKPISAVAEAIEIIQNLTRGLKKPFHGEIFSLREGAFFRWNADHGKIPVMVGTW
                     GKKMSTLAGEKADEVKASPLWNSDYARTLRSQIDQGAISAGRAPSEIGLTLGVLTSIH
                     NDSDVAKEYARNSLAIYLPHLYPMTEVMGVPANEIANVRKLSNLGKYSEASKLISDET
                     LNNFSLYGSPVEIRSKITSLIDTAPVDKIEFGTPHGPNSFEAIILLKNEVIPWFS"
BASE COUNT         5175 a         3868 c         3896 g         4914 t
ORIGIN      
        1 tgggacccct aacaggactc gatcctgtgc ctccggtttg ggaattgtac tgaaggtgtt
       61 ttaaacggtc attttaaaga ttagattatc tatatttgta ctcagcacaa tggttaatct
      121 tcactaacag tcttagtcct tatcggtaca tcctcagaat ttgtctaaat tatatttctg
      181 gccgcatcaa tcaaaagata gatggatatc agagctaaca aaataccact tgctcgtagt
      241 attagagatc ttctttttga aattgttgaa accattttat tgttaccaat aactagggct
      301 acggttgtgt accatgctcc atcgataata ccggctgtca aacccatgat gaaaatttcg
      361 acccaatttg aacctgtatt tacaaactga ctaaatatcg ctaagaaaaa caaggctatt
      421 tttggattca ttatggaaat tccgaatcct tctagagcgg ctcgtgttaa tttgcggttg
      481 ggtgctaggg tttcaagagt tttaggtgaa ggaactgatt ttgaagctgt tatggatgtt
      541 cggtagccaa tgtataggat taagcaagcc ccgataattt gaaagatatt aagtacaact
      601 tcgtaattca atatgatggt tgaaagccct acaacggtta gcatcgccca tattacgatt
      661 ccgaaggcgt gagatattcc ggtaactgaa ccgcatatct tacctctgtt tattgtgtta
      721 gttactacga cagcaaggct tggccctggc gtgatagccc ctaaaaaaca tataaatatg
      781 atttgtaccc aggaagttag ttccatgatg caatgtttct ccctggttca gtcatagcaa
      841 aatttccgag caaatatcta cctcaagttt gatcacacac aaaacaaatc gggagtggta
      901 cccctaacag gactcgaacc tgtgcctccg gtttagggat tgtactaaag gtgttttaga
      961 agatcatctt aaagattaaa tcggctctat ttgtactcaa cacagagcct attcttcact
     1021 aacagtctta gcctttattc atacatcctc aagagtttat gaccattttg tgaccataat
     1081 tcaacctcct atttaaataa ttgaccttac gaataatttg attgctgatt ggttctttgg
     1141 cggtatagcc aagtaaactc ttctttataa ttaaggtaag aggtgaatat ggttggagat
     1201 gttatatacg gcagggtggt attgcatgag agctctgtat ttagggataa gaagtcctcg
     1261 tctgattatt caatcgtaat taccttgccc gaaggcgtgg acggtgaata tgttagccca
     1321 gtacctataa agataagtat tggaatctgc ctgcgaattt gaaatatatg actcaccgca
     1381 gagtttttga tgctgaaggc tcacatttca ttgatgaaaa tattcactca agtttttggg
     1441 aggcacaggc tgactataaa agaagatact tcagagtaac cgagccaaca tcctctaccc
     1501 aaaatgcaaa accacttcca ccagaaaaag agagcacctt cttttaccga cggagaaaga
     1561 ctctaacagg aaccctaatt ggggtagcaa ttctatttta ttttgtcaga tttgctggct
     1621 atttctgcta tcagaaatat ctacagtcga gggtgttgat tcaggtaagt tttctcttgc
     1681 ggtttcggtt tcaggcacgt tttcagagtt ggcaatattg gcaattatcg ggttattatc
     1741 gtccaattgt gcgaccccag tagatgcatc tttcgacccg catctgaatg ctatagcacc
     1801 tattagacag gcagcaacca tagggatcat cagccttaat cggggtcgtg ccataatgat
     1861 tttcatcttt tcacaatacc ctcttcccac ttagttcgac caattgatta cttcaatatt
     1921 atgagagaac tttaaggaaa ttataagaag ttttcacttg tagtggaatc ctagagtcgt
     1981 aaaagtcata ccaaataggg aaataactca cgaattgcaa ggaattaaag actccaatat
     2041 atatatcgtt ttttaggggt gatacaatcc aaaaaatacc ggagacagct tagttaaagg
     2101 aagggttcgg catgccagaa ggtaaggaac tatatatgct tatgcttagg cgaatgatgc
     2161 tcattcgcaa gttcgatgaa acagtgaagg atttagttca aagcgccgag ctagtcggca
     2221 tggctcactg ctatattggt gaagaggcag tagccgtggg tgcctgcacc gcactgagag
     2281 acgaagacta tataactggt aatcaccggt ctcacggtca tccaatatca aagggggggg
     2341 atgttcgaag agcgatggca gaattgctgg gaaaggctac aggctattgt aagggcaagg
     2401 gagggtctat gcatttagca gacttcgata tcggcatttt aggagaatcc gggattgtgg
     2461 ggtctgcttt acccgttgcc gtcggtgcag caatgggaag caaaatgcaa aacaacgaca
     2521 gagttgtcat ctcttttttc ggggatggcg ccagtaatca aggagcatgt catgaagcta
     2581 tgaacctggc ttcaatatgg aaattacctg tcattttctt atgcgaaaat aaccaatacg
     2641 ccgtcaccac ctcttttaag gacactgttt caacggaaaa tgtttctgac cgtgccactg
     2701 cctataacat gccaggagta ttagtcgatg gacaggatgt tatggccatg catgaagcaa
     2761 ctgtacatgc aattcaaagg gcccgagctg gtgaagggcc gaccctttta gaagccaaaa
     2821 catatcgata tgaagaccat tcagaaggtc tcaacagaat tctcagggag ccttacagaa
     2881 ctgaagaaga agtcgaattt tggcgtcaaa gagacccgat agacattcat tcgaaatggc
     2941 ttttggaaaa cgatgtagcg agccaagaag agattgataa agtacaacat gatgtatatg
     3001 aggctatagg tgatgctctt caattcgcta gggagagccc gtatccggat gaagcagatt
     3061 tatttactga tatgtttgcg gacccaatcc cgctccgcta accattaatt gtcggactta
     3121 taacaaaatg atctgatgaa aggggaattt tatgggtgaa attacatatt ccgatgcgct
     3181 taaccatgca ttaaaggaag aaatgcggag agatgaaaaa gtgttcctca tgggcgaaag
     3241 tattcgaggt ggtatttatg gtgtgaccgg tggcctttca caagaatttg ataaacgggt
     3301 aattgacact ccattatcgg aaaatggatt catgggagct gctgtcggag ctgccgctgt
     3361 ggggatgcgg ccaatcgttc aatcattagc tagtttcgct tgggttgcca tggatcaact
     3421 ggttagccaa gctgcaaaga tgcgttttat gtttggaggt caggtcaatt tacccatagt
     3481 ttatcgctgg gggctgctgt atggtaacaa catagccgct caccatgccg atcgcccata
     3541 tcccatgttc atgaatatgc cggggttaaa gatcgctata ccttccaccc cagctgatgc
     3601 caaaggattg ctgaaaacag cgatccggga taatgaccct gtcatgtttt ttgaggataa
     3661 taatttagcc ggcagccgtg gcgaagtttc agaagatccg gaaatctgca tcccgttcgg
     3721 aaaagcagaa gttaagcgag aaggagcgga tgtaactgtt gttaccatcg ctggaatgct
     3781 gagaccagcc ttggcggttg ccgatgaact agaatctgat ggcatatctg tcgaggtgat
     3841 tgacccacgg acaatcgtac cattggatac caaaaccatt ctagactctg ttgaaaaaac
     3901 aggcaggtta gttgttgttg accctgcgca tcgaagctgt agtgtggcgt ctgaaatatc
     3961 tagcatagtt tctcaagaag ggttttggag ccttcaagcc ccaattcaaa gagtgaccag
     4021 ccttgattgt catttccctt ttagcccagc attagaacat cttgtttacc ctaatgaaga
     4081 taaaatcgct gatgcgataa acctaacact tgaatgaaaa taacccgctt aaccgagcaa
     4141 ggaaatagta aggtgtaatg catggccatt aacgttgccc tccctcaatg gggtatgaat
     4201 atggaagagg gaattctggt taagtggtta gttgatcaag gcgaagaaat ccgaaaaggc
     4261 cagcctttag tggaggttga gacttccaaa attagctctg agttggaatc accagctgac
     4321 ggagtgatag cacatctaat ggcgccggaa ggagctgtcg tcaaagtggg agatttggtg
     4381 gcggtcatcg gggaaccggg ggaaaatcct cctagaccag agaagacagc atctaagccc
     4441 gggagaagga agtcaactca agaatcccgt tttagcctga ataaaccaaa tagaataaaa
     4501 agaacccagg ctaccccaat tgctcgaaaa ttagctaaag acaataacat tgatattgat
     4561 gaactgaccg gatccgggcc aaatggaaga atttctgaag gtgatgtaag ggaatacatc
     4621 aaaagggaca aagcaggaac aaggacaaaa accggtcaaa tttccccaag agccagaaga
     4681 ctcgccttga acaataatat cgatctgaat cagatttcag gaactggccc caataggcgt
     4741 atttccgttg cagacgtgga aaatgctatc ggttcaatcg ctgacgctgg tgtagatgaa
     4801 aagtcgatag gcgcttctca aacattatct ggcttgagaa aaaccatttc agaacggatg
     4861 tccctcagtg tgcgaaccat ggcacaggtg acattaacat cagaaatcga tgctacgaaa
     4921 atggttggtg ttaggaaaaa tttactccaa tcttggcgtt ctcataaaat acgtccaatg
     4981 gatcaagata tcctggtcgc agccgtcggg gatgccctca caaaacatcc tcgtttaaat
     5041 tctttcctta aaaatgacac ggtgttattg gcaaaggaca ttaatgtggg catagcaatg
     5101 gctgtacctg atggattgat ggtgccggtc gttaattctg taaatgaaac cagtctcatc
     5161 gaaatagcaa aaaatctccg agatctggca aacaaaactc gaaagaacag tttaaacata
     5221 gccgaggttt caaatgcagg gtttactata acttctttga tgaatcatga catcgacgtt
     5281 ttcacccctt tggttgaccc gcctcaagtc gcgattctag gaatcggaag aataactgat
     5341 aaacctgtag tggtcgatga tgaaatcgtt atcaggcaga ttatgcatat cagtgtaact
     5401 tttgatcatc gagctttaga tggtgttccc gtcgctgaat ttattcacac cctaaaaagc
     5461 aaccttgaag accccgagtg gctgaaggaa cgttgcctga acaatcaact accttcgaga
     5521 cgcaattaaa attgctaagc catatgaatt tattgtccgg ttgtaattag caataagaga
     5581 aatcaccctt tgatcctaga catcatcgag gtatagaact gggatttaat ttattttcct
     5641 caggagtgct attatcagct tagcaagaac tacggacgat tcagcttgaa aacggaggta
     5701 taaatgttac ctataagcac ggaaccgatg cagattagaa cctcaaatat tattgattca
     5761 aaaggagacg gtccatggta tgaagcccta ttctcagatg gacgcaataa tgtggggtta
     5821 atctgtgaca ctctaggatc ggtaaacgac gatcactacc atcctgactt taatgaattt
     5881 tggatcatac tcaaaggttc acttgagttt gaaataggtg atttcccgac catacatgcc
     5941 cacaaaggag atgttgtact agctcctgct ggagaaagac atcttataag aactattggc
     6001 aatgaatctt ctgttagatt gcatgtaagc aagattggtt ctgatcatgc caataggaaa
     6061 gatcgaagta atcagacagt cccctaccct actcaaaaag aaccccctaa ccttctacat
     6121 accaccttgg actcagtaat agaacaactg ggcgaaccac agtgggtaac tagcatcatc
     6181 aaggattcat taaatactgc caatctggta tacagtgctc ctggtacacc acatgacgcg
     6241 cactggcacc cagaattcga cgaatggtgg acaatcctga agggggagct aacctggaat
     6301 ctgggtgaaa aacgccccgt ataccaggta aaagaaggcg atatcatgta tttgcctagg
     6361 ggattgaaac atcacatact tacccaaggc tcggaaactt ccttcagatt ggcaataact
     6421 gattccgaag ggctccacgt tttccaggaa ggcgatgaag atgcgcctcc tcctcgggaa
     6481 tagaaccttt aaggctacta tttcacatgt catctcaggt ttggaggcaa ccaccaaatg
     6541 aattgacgtg tttaaatgga gtgaagtctc tatcaggact aaaactttgt acattcggtt
     6601 taggaatctc tttgccagtc aaccgtaatg catacggcga cctattagct aatataacga
     6661 aatctggcaa attaccataa gtgtgtttcc tgaactaaat cctgtttgac taagggttca
     6721 gatatatatt gatactttga cgagaatatt cactccagtc tcgcgtcccg gtgctctctg
     6781 tctatttctt ccagttagcc cgttctgcct cgtaggcccg gctcgcattg tgcaatgcgc
     6841 tttccctctc ctctgccaga gtgtctctca tgcctctgaa gaaatcgaaa gacatgtcca
     6901 gactagccgt gctgccttgg aatttgggca tgacgtacct ggcggccaac tcgtagctgt
     6961 ttttcatctg ttcatgggta ccgagctctg ttgccaagaa aatgaacttg ccgaagccgc
     7021 cactttcctc atcgagacga ttgatagagt ccacgaggtc gtcgggagtg ccaatacacc
     7081 aaataccctc gtcgaccatt ttatcgataa tctggtcggc cgggccatcg atcggtgggt
     7141 tgcccaccac tttatcgaag tactccctca tcaacagccc cgaattcctg cgggcgatgg
     7201 acatcgcctc ctttttggag tcggctaaga acacatgctg cactaagccc cagttttcgc
     7261 ggttcatctc ccggccgaat tcggcagcag actggacccc gatctcccaa agatctttga
     7321 gagtattgtg atagaaaccc ccaggcgaac gggcgaacgt gagggacacg ggggttgctc
     7381 cgtgctttcc ggcaagcttc atccctatcg gtgattgggc tgaggccacc atgatttcaa
     7441 agtgaggttg gctataagga cggagttgaa gatgcgcgtc taccaactca aaccagtcgg
     7501 aacgataggt aaacggttcc tcttcggtta agagtcgcat gatcacgtcc agtgactctt
     7561 ccatgcgagg ccgcgcctgt tcgtggggta ggcccagcat gtgcccatcg gaattgagag
     7621 cgcctggtcc tacccccatc gctgttcggc ctctcgtcaa atggtccagc aggaccattc
     7681 tattcgccac tatcagtggg tggtgatagg gcaaaggaat aacgccagtg ccaagcttga
     7741 tgtgcttagt gcgctgcgcc gcggttgcaa taaatatttc tggagatgag atggtttccc
     7801 aaccacagct atggtgttct cctatccaag cctcatcgta accaagataa tcgagccatt
     7861 caagcaactc aaggtctcgt tctagtgcca atgttgggtt ttcaaacggt gcatgaaatg
     7921 gtgctaggaa tattccaaaa cctaatctat cgggtaaaga catattcacc tccctaattc
     7981 caattatctt ccacctgaat caatttcaat ttttccaaac aaccttcctc cggtatatcc
     8041 taccaaggta tttagaaagc cttctcaaat atgaggctcg tctgcgttga tatcacaatt
     8101 tcatcatatg gttggagtca tatcttataa gttgttcatg gtctggaatt gaaaaacaca
     8161 taagggtaca tgaccttagg cattccgcag tgagcttagc ccttctcaaa ggggtccctg
     8221 ggaaagtggt tcaggaaatg ctcgggcaca cactattcat ccacgatgga tatctatagc
     8281 cacgtcgacc caggtatgca tatggaagct tcagagaaga tgaatgaagt cttagaaggc
     8341 tagcatttca aaaaaggagc cacatattta agaaggcaat cggtacatat tcaaatcaga
     8401 agtccaattc tcttacaggg gcaatatcac acttggtcca gcttaaagca ggcctcaaaa
     8461 attcgtacaa cttatctact tcatcagtat ctaccacata gacagcacga tgctctggtc
     8521 tgcctccgag ggctaaaatg actttcactc ctgctttttc tgccccctca gaagtaatca
     8581 tttttcagga gaattggtta ctagtgggca actttctacg gtgtggtcaa cagttacatg
     8641 aaataacatg ttttccctcc gtaatttcta accattatta tatttcttgt attcacgaac
     8701 ctgttctgta agctatacga cttagagttc taaacattct caccgtcaat gacgaaacct
     8761 tgcttctaaa aggagcaaag ctttcaccgt ttacctcagc aattatgctg tttacatctt
     8821 caagtgagtt tattccatta actattcgca ctacatcagg attaccaaac attggaacag
     8881 aaataagagg gtccatcgtt ttagatgaat ctgccatctc aagaacagta tccagcaaat
     8941 ttggagcttc acccctcttt gcaacaaaaa tagttcttat catgtactta gcctctggag
     9001 aaaatacctc aggctgtctt acattgcgga ttatggagac atttacatct tcacaatctg
     9061 ctgccaattt ctgaaactta tctcgagact ggtcattttc aaggaatcca tcgtggaaag
     9121 cttctatttc tcccaacgta tcgaatgtta aggcgcttat gaccctactc ttaagagcgg
     9181 gaggcgttga gattgtgaaa gagacgttac cattttgatt aagaccttta attaaatcaa
     9241 tcgaaccctc tgttacgtca aagttttttc cttgctttgg tatcctacta acgttttgta
     9301 tgaattccgg cataataagc tccttttatc tgaattattg tccgtaacac aaaataaccg
     9361 ttttactcgt tattggcaaa gtatcggctt aaatctctca tagtatgtca atagaacctc
     9421 aaggtttaga ttaattggac tttgtagatt tagctgtaac tatgaattca gccgagagtt
     9481 cttgaccatt ttgtgaccat aatttatatt ggcccaagag gttaaaaagg caaccctggc
     9541 cagactggaa tctgtgcctc aggtttagaa gaccggcaaa gtcagccttg ggtcgcacag
     9601 atttctgtca aaattcccta tcgccagtac cctaatagca ggaacctctc gttacacctc
     9661 agccttctga atcaggctct tttgccaaaa tttactgtca tcggtattta tgttcaggtg
     9721 gtctcaagga tttatactca tcatttgtta gtatccttga cactgtcata aacctcaatc
     9781 cgaggggaat ctctaacgaa aaactcgatc ctcgtccttg gcaaatgtca atggtgatat
     9841 gggaatattt ataatattca aattgatctt cggccataaa aaactcgcat ccatgaatgg
     9901 tgccgaggga taagtctata gatccgacaa tgaattcacc ttttttaaaa cacatgggtg
     9961 cagacccgtc acagcatcca ccactttgat gaaacataag gtcgccatgt ttctctcgta
    10021 attgatctat aacctctttg gctttgtcgg ttacgctgat acgttcggta tacataatag
    10081 atgcgggtag tttaaactgc ccgttccctc ttttttgatt taaaagaacc ctaaaggatt
    10141 tttatcatag gatattaata tgtttttggt atggcgataa tggtcgagca tcattttatg
    10201 tgtctctcta ccaatcccgg atttcttata tccaccaaat gaagcatggg ctgggtatgc
    10261 atgaaagcag ttacaccaaa ctcgacccgc ttcaatacca cgggacatag actgcatttg
    10321 atgaatatcg cgtgtccata ctccagcgcc taatccataa agagtgtcat tagcaatttc
    10381 caatgcttcc gcttcatctt taaaagtagt aacactcaag actggtccaa aaatctcttc
    10441 ctggaaaata cgcattttat tattcccttt aaatacagtg ggtttaatat aataaccatc
    10501 tgggtaggtt tcattttggt aggcttcacc accgataagg acttccgcac cttcttgttt
    10561 cccaatatca atgtaattca gaatcttctg gtattggtca ttagaagctt gagcacccat
    10621 catggttccg ccatccaaag tatcacccat ggtaatggct tcaattcgtt ttaatgaacg
    10681 ttccataaaa gcatcataaa tattttcttg aatcaatgct ctggatgggc atgtacaaac
    10741 ttctccttga ttgaatgcaa aaagcacaag cccttcaatg gctttatcaa ggaaattgtc
    10801 atcttccgcc ataacgctat caaagaaaat attgggagac ttcccaccca attccaatgt
    10861 aaatggaata atattttctg atgcatattg cattatgaga cgaccagtgg tggtctcgcc
    10921 cgtgaatgca attttcttta tgtcggggtg cttagctagg ggttgacctg cttctggtcc
    10981 gaatccattg acaatattta ccactccttt aggaattaga tcctcaatta attccattag
    11041 aagaaggatt gaaacaggag tttgctctgc aggttttaaa acaacacagt tgcccgcagc
    11101 taaagctggg gccaatttcc atgcagccat taaaataggg aaattccatg gaatgatctg
    11161 cccgactaca cctaatggct catgtatttc cattgataca gtattggaat caagatcaga
    11221 catttcaccc gactcagcac gaataactcc tgcaaaatat ctgaagtgat cgattgtcaa
    11281 tggaagatca gcagctgttg tctcacgtat agcttttcca ttatcccagg tttcagcttt
    11341 ggctaaataa tccagatttt cttcaacccg atctgcaatt ttatttaata gcgcacttct
    11401 ttcagttgca gatgaactgt tccattcggg agcagctttc catccagcct taacagcgag
    11461 gtcaatatca tttttatttg attttggaac tttagcaatt agacttccat caactggtga
    11521 attattatcg aaatagtcac catctaccgg cggcacccat tctccaccaa tataattctc
    11581 gtactgggat ttaaattcgg gtctatttga catatttaac tccaaattgt ttttcttatt
    11641 gactcctgga taggatagtt gcggttgata ggtgtattgt caccatttgc attgaatatg
    11701 acaagtgacg gatgacaaac ccaaggtacc tagcgctacc cctcaggaag gtttggaatc
    11761 tgtgcctcag gtttggaaga ccggcaaagt ctgtcttgtt tcggacaggt tgctgtcaaa
    11821 gttattattt tcagcttgta catcatggtg aagaaccaca atacagtcta ttccgtgact
    11881 acaaggaatg gtacccctaa caggactcga acctgtgcct ccggtttagg aattgtacta
    11941 agggcgtttt agaaagtcat tttaaagatt agattatctt cacttgtact caggacagag
    12001 cctatccttc attaacagtc ttagccctta ttcatacatc ctcaatagtt tgtgaccatt
    12061 ttgtgaccaa atatgaaacc cagtaggtag caagaagcta tgagtaaatt ggacgcttta
    12121 ctgactagct aaaattagaa tgatattaag ctagttggaa attttcagtg taggttaata
    12181 tcattgtgga atcatataag acatataaag gaagtgtagg aatgaaaaaa cttcaggaac
    12241 gcatagccca gggtgaggtc attattatag acggcccgat gggcactgag ctcgagcgtc
    12301 gtggtgtgcc gatgcacggc gaggcgtgga gcgcggcggc cctatacact catccggaag
    12361 tggtacgcca ggcacatgag gactatatcc gcgcgggtgc agacattatc atcacgaaca
    12421 ccttcgcctc cgcacgacat acattagaac gcacggagat ggcagaccag gtgcgagagc
    12481 ttaacacccg cgccgtcacg cttgcaaaag aagcgcgcga agccacagca agcgaccgcc
    12541 cggtctatat cgccggttcc atatcgactt ctttcagggg tggcactcaa gaagcgagtg
    12601 ccaattacag ggagcaggcc gagatactcg ccgaggcggg cgctgacttc cttattatgg
    12661 aaatgatggt ggacgttcag cagtccgcgt acgcaatcga agcggcggtg tcgataggga
    12721 ttccggtttg ggtgggattt agctgtaaaa tacgagacga tgactctcaa gttgtttttc
    12781 gggagctccc ttggacgcac ggcaaggacg aggacaagac gttcgcggag gctctggact
    12841 ccctgctgcc cataggaggc tcgttggtgt ctgtgatgca ctccgacatc gaggccacgg
    12901 cgcctgcgtt gcgtgctgtg caagaacgct gggatggacc tttaggcgct tatcccgaat
    12961 ctggcaggtt cgtgaatttc agctggcagt tcgagaacgt cattcctccg gaagatttct
    13021 tgtctgaggc caagaagtgg gtgcagatgg gtacacaggt agtcggggga tgctgtggaa
    13081 ttgggccgga gcatatccgt ttgctgagag agggacttcc gtcgcacgtt ccggcggaac
    13141 aacaatccgt atcgtgacta aaaagagtgg tacccctaac aggactcgaa cctgtgcctc
    13201 cggtttagga aaccggtgct ctatcctcct gagctatagg ggcataaatg aattcccgat
    13261 gcaaaattgt atcaaacaga acctgactta ggtttgctta agccgatatt gataagaact
    13321 tcgtgttatt acagaatgta ctcatagaat ttagcccgtt taattttctc tctggagctc
    13381 ctaatatgtc gcaaaaaata gaaaaagtcc ttataaccgg tgccacaggg tatgttgcag
    13441 accagatgct cgcattcatg aaagagcagt atgaaactgt tctagtagat accagagagg
    13501 taaacaggcg cggagaacca atagaaggtg taaacatcgt ggatctaatt gatccacata
    13561 gaaacaaata ctccgatctt tttgaagggg tggacgcggt tatacacctt gcgtacaaaa
    13621 gaagatcagg ggatcccctc gatcatttca atgatgaaaa acaaaatgta gaaatggcat
    13681 ataacgtctt tagatcatcg tatgatgccg gtgttcaaag ggttgtaatg gccagttcaa
    13741 atcatgcagc cgactggtat gaacacgctt taatacacaa cggtgggttg gatatagtcg
    13801 ggccatacga tttaccactt tcagacaatt tctacggatg ggcaaaagcg acctatgaac
    13861 atatgggatt cttgtttgcc agcggcggta tgggagaagg aggtgatgga gcatccggca
    13921 gcgctgcaac aggaaacctc cttgcgggaa atttaaacac cagcagaaaa atgggtgttg
    13981 tgatggtaag gatcggtgct cctcgggata tagagacaga aatatacaga gggaaagcag
    14041 cggcttataa aagagatctt ggtgcttata taagtcctcg agacatcaca caactttttc
    14101 atagggctat ggaaacccca aatatagaga acgaatatgg agttccatgg caggtggttt
    14161 acggtataag caacaacacc cgagcgtttt ggtcacttac tagcgcgagg aaagtattgg
    14221 gttacgaacc tgaggacgac tcagaaatta catttagggc cgggatcaac gagatcctta
    14281 acgattcagg acgtgttggg cctttaaatt agatttccag gagcaaacat accgatcaaa
    14341 aacttcagat tctctatagg cgctttaata tttgcccttc tagctcttta ccatcagagt
    14401 atctcttgag gttctcgaca gcaaaatcgg cacgtttacg catcatgcca tcagtgccag
    14461 ccactgtgtg aggttgaact ataacgttgg gtaatttata gaccggtaga tcaaattcag
    14521 ggggttcttt tgcaaatgca tccagaccag caccggctat ttttttatcc ctgagggcat
    14581 cgtataaggc atcctcgtct ataagaccac ctcgagcaac attaatcaca aaagaagttg
    14641 gcttcaagag agcaatcctt tttctattga ttatgtgttt cgtctcttcg gttaaatgga
    14701 gatggacaga taaaaaatca caatccccta tagcgtcatc aagatcttcg gttcctccca
    14761 ggaaatcagg ttgtatctca tctaagattt cctgttcgat tggacgcaca tcaatggcat
    14821 taattttcat accgaaagct ttagccctac gtgcaagttg ctgcccgcta gcgccgaacc
    14881 ctacgatcgt taaagttctt ccctccactt caaggccgtt ggcactgaaa accgttccag
    14941 caaaaaactg atcagttgca tttccataat catgggcaag catgagagta aacatcatgg
    15001 ccccttctgc caaggaaaca ctgcttaaat ggcctgggca atgagcaagt atcatccctt
    15061 tctcaataat tcgctccacc tcaacatgat ctaaaccatt tgtctgaacc tgcacatatt
    15121 taatccctgc ttccgccgcg acatccacta attctggttc tatatttcca ccaagatcta
    15181 cgatgacttc cctacccgtc agctgtgacg gccaatcttt tgatctatca aatatttcag
    15241 tatcgaattc cttcagcaag atttcaggca agtattgatg tctttcttca acgctaatga
    15301 tcggatgtgg caaatatgaa acctttaatt tccccatctg tcatacctcc tctaaatatc
    15361 tatcaaaccc aacaatttca gaaaacacaa aacccggctc tatcctacat aaaatcaaat
    15421 cccctagcga gatgacaagt ttgccttatg ccgatatcct accctcaatc aagaaatcta
    15481 catggattga taattaaaag gatagcaaaa tgccagaatc tacattgacc attgattgcc
    15541 aggtacatgc gtatgaaaag gatagtcttt cacggccatg gaaagggttc ctacagggcc
    15601 ctgacgaagt aactggggat gacatggttg cagcaatgga ttcagttggc gtggatggag
    15661 cgattttgat atctcccgca agcctatatg cctatgacgc tagctatgca ctggaagttt
    15721 atgcaaaaca tcctggtaaa tttggcttgg ttaagccttt tgaccctgaa tcggaaacag
    15781 taggtgaaga agttgaagaa tgggcagcga cccctggtgt ggttggggcc cgcataatgt
    15841 taaggccata tgagttcact gaataccatc caggtctgag tagcattctt gatgctggag
    15901 ccgaagcagg aataccgata aacattatgt gttcaggaaa cctagatttg ttcagccaac
    15961 ttgccgacaa gcaccctaac acccagatgg tgattgacca tctaggaata ccgcaaccac
    16021 atcttcctcc agtaccagaa aatccatttg cggatttgga caaagtcata tcactggcaa
    16081 agcatgacaa tgttgtcata aaaatctccg gtgccggtac tttatcgcat gaatctttcc
    16141 cttatgcgga catatgggga cctttaggaa aaatatttaa tgcctttggt ctggatcgtt
    16201 gcctatgggg aactgattgg acaagggccg ttgaactttt gacatacaag gaaggagttg
    16261 aggcattcag agtcacagat tcactatctg attcagaacg agagatcctt atgggtggca
    16321 gcctctctaa aatctataaa tggtcaccca aaaaataggt ttaacttctt accgatcgta
    16381 agctaaaacg acaaccatga atcttgttct tgtggttccc agtattccac ggtgactgat
    16441 tcaataatga aatgttcata agggtctacc gatttatatt cttcgtaagg cccatcccaa
    16501 ttcttcctac gttccttata ggaatcgtgt ccttccagag aatggaattc aaaattcatt
    16561 atgtagcgcc cagtagggtt tccagaggaa tcgagttgac gaaaatattt agcagatttc
    16621 cattctggaa ataatttgtc gtgatatttt tctacaaata agaaccactg tctaatgatc
    16681 tcatcatggt catcctggtg accctgttta atttcccagc tttcaacttc tatgaaactc
    16741 aacttatagt gctcctgata agcattgatc tgacataata ttggaacaaa ttctgatgta
    16801 aaggatcaaa tggtacagtt cagtatagca atcagcggcg accgggagat caaagaatat
    16861 atctccatag caaaaattgt cgatcagtcc gacttctcga acttaagcat ctatgacgac
    16921 cttatgttca aaccagcctg gccaatactc agcataattg ctattaacac agaaagaata
    16981 aaaatcggcc ctgctgtatt aaacccctat ctgacacatc ctgcagtgat cgcagccaat
    17041 atctcagtaa tcgatgagat ctcaaatggc agaagtttcg ttggcatagg aagaggggcc
    17101 tttttagatt ttctaaacat aaaatcggaa aagcctatta gtgcagtagc agaagccata
    17161 gaaataatcc aaaatctcac aaggggtctc aaaaaacctt tccatggtga gattttttct
    17221 ttaagggaag gggctttttt tagatggaat gctgaccatg gaaaaattcc agtaatggtt
    17281 ggaacctggg gaaagaagat gtctacctta gcaggggaga aagcagacga agtaaaggca
    17341 agccctcttt ggaacagtga ctatgctcga accctacgct ctcaaataga ccaaggtgcg
    17401 atctcggccg gaagggcacc aagtgagatt ggtctgactc tgggtgtatt gacatctatc
    17461 cataatgatt ccgacgtcgc aaaggaatat gctcgcaact cactagcgat ttatctaccg
    17521 catttatacc cgatgacaga agtgatgggc gtacctgcaa atgaaatagc caatgtcagg
    17581 aaactttcca atttaggaaa atattcagag gcatccaaat taatttccga tgaaactctt
    17641 aacaattttt ccttatacgg ttctcccgtt gagatcagat cgaaaatcac ttctctaatt
    17701 gacaccgctc ctgtagataa aattgaattt ggaaccccac atggcccaaa ttcctttgag
    17761 gcaataattt tactgaaaaa cgaggttata ccctggttca gttagtgccc taatattgag
    17821 atatttagga atctgaatcg gtaaaccagt ccc
//
LOCUS       DASCNS010000215         1122 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001649163, whole genome shotgun sequence.
ACCESSION   DASCNS010000215 DASCNS010000000
VERSION     DASCNS010000215.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001649163"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            108..617
                     /locus_tag="QF838_07930"
     CDS             108..617
                     /locus_tag="QF838_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60644.1"
                     /translation="METALIVAIAQIATGIATLVVALFLAAQLLIQKSQLEIAHQDSV
                     RELGFAARTRNEELILARLTNKSLLNSYLKVGAGLETPSDEETHQFMNYMRLSYLQMI
                     NEWRLGVNDQNVEYFKGRLGVLMGSVGERRYYLTNGKIIVGTVFGLSDLVNLGDMVYE
                     ELQGRPVPA"
BASE COUNT          334 a          205 c          272 g          311 t
ORIGIN      
        1 acaatggcta atgatagttg tggactgcaa ggactaaggg gtactaatct agctgtaact
       61 actatttatg tgacattatc cttctgaata catttcagga ggatgatatg gaaacagcgc
      121 taattgttgc catagcccag atagcaacag gaatagccac cttagtggta gccttgttct
      181 tggccgctca gctgctaata cagaaaagcc aacttgaaat cgcccatcag gattcggtaa
      241 gggaattggg atttgctgct cgaactagaa atgaagagtt aatactagct aggttaacta
      301 ataaatctct tctcaattcc tatttgaaag ttggagcagg ccttgaaact cccagcgatg
      361 aagaaacaca ccagttcatg aattacatgc gcctttcata tcttcagatg attaacgaat
      421 ggcggttggg tgtgaatgac caaaatgtcg aatatttcaa gggacgatta ggtgtgttaa
      481 tggggagtgt tggcgagcga agatactacc taacaaatgg aaaaataatc gttggaaccg
      541 tatttgggct tagcgattta gtcaatttag gcgacatggt ttatgaagaa ttgcagggaa
      601 ggcctgttcc tgcataattc aaaatctgtg tggcgatagg ctccatgtgt agataggcgt
      661 aaaagacgaa ccccttaata cattcctgct agagctggta ccattaaatg ggcacctcag
      721 ttcctggagg tctagaaata gacgcgaaca ctccatgagc gtcaagctca ctggggctgg
      781 ggtgaccgtg cacctactga ttaagaatca gtcattgtgg aattacgtgc gaatgtttgg
      841 tggtgacgcc gctgggacgc tacccgtcat gattaattag gattcagtgc cttggttaaa
      901 cctcgtatta gctcggtgaa ttcagctaca atttgattat aagagttatt ttgttgagag
      961 tggttttatc tttccttctt taagggaaaa gatgattttg caaataataa cgaggccgaa
     1021 accgaggcaa gggctgaagt gattaaagca aaataaactg attttacgac agatattatg
     1081 gccattattg gaccagccga catttcccca aaagatgtgg at
//
LOCUS       DASCNS010000216         6204 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001649231, whole genome shotgun sequence.
ACCESSION   DASCNS010000216 DASCNS010000000
VERSION     DASCNS010000216.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001649231"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(24..1157)
                     /gene="rlmN"
                     /locus_tag="QF838_07935"
     CDS             complement(24..1157)
                     /gene="rlmN"
                     /locus_tag="QF838_07935"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HJO60645.1"
                     /translation="MVNGRKISAIEATLPSILELSKSNLLASLEALDQPKYRAEQVWR
                     AIYKEGRTSFGQMTNLSKDLRGILAQNYSIGNAATVISKKSADNSTDKILFKLPDGEL
                     IETVLMRYEPDGHRRGRKTVCVSTQAGCALGCTFCATGQQGFRRNLTAGEITEQVLTM
                     ERLALAEDKEEIQAGTRSRGELQGVTNVVFMGMGEPLANYENMMESIWVLNDPKGIGI
                     GARHITVSTVGLVPGIEKLAKENIQINLAVSLHAPDNETRSETMPVNKRYPIEELIEA
                     CKYFVTKTNRRIFIEYVLLKDQNDTSMHANKLGRILKDLLCHVNLIPVNPTDDGPYHR
                     TTYKEAQRFQSDLKTFNVPSTVRMEKEKGRETKCADGADDRYR"
     gene            complement(1187..2059)
                     /gene="rsmI"
                     /locus_tag="QF838_07940"
     CDS             complement(1187..2059)
                     /gene="rsmI"
                     /locus_tag="QF838_07940"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HJO60646.1"
                     /translation="MKNSSTAGFEVTDNGDTLMGTLYILGTPIGNLKDITFRAIDILS
                     RVTLIAAEDTRVTQKLLARYDIKTKKTSFNKDNYAQKIPEILEELGRGDIALTTDAGT
                     PGISDPGYQLVKAADEAGFTVMGIPGPSAVTTILSMCPFPTDSFLFAGFPPRKKSDLV
                     KFLSQYEALAVPIVLFESPKRLKNLLEKLAHLYPERSIFVGREMTKVHEETFHGKPAA
                     ALIHFSEPKGEFTLVLTKSDVEIEHLDRSATLTLIQKLSDEGVGIRDISREIVSVTNL
                     GNSEAYKLVLDTLE"
     gene            complement(2049..2744)
                     /locus_tag="QF838_07945"
     CDS             complement(2049..2744)
                     /locus_tag="QF838_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60647.1"
                     /translation="MSTYVLIHGSYQGGWIWKPTAKELVKKGHTVYTPTLDGCGERSI
                     SIRPGITIKSQANEVSGFLNYQDLTDLILVGTSTGGLVTCKVAELSRSRVKEIFFVDA
                     LAPQPGERVQDIVVRDPSFPQTITELTRGPSEAELEERLFQDLDPELKKWAIERATMH
                     PIEATDAPGELDNFWNRNWNATVIRCTLSSNPSREHQKRTAEKLNAKWHELEAGHYPM
                     LSHPKELSLLLEK"
     gene            2834..3973
                     /locus_tag="QF838_07950"
     CDS             2834..3973
                     /locus_tag="QF838_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60648.1"
                     /translation="MRKSIKIPFLVIFLVLLLLITPQARASFKAALFIPQILPAIPIK
                     PLEYVSGTPTRLEITFESSKGFSEADIYLPSGNGTHPGVVFFMGIVPPDREEKRILAL
                     AEGLARTGMVVLIPWLETQAENRLVVDDIETLVDAFLYLQEHERVDGTRVGMGGICTG
                     ASMAVVAAQSNRINEEVTFVNLFAGYYDAFDLVKSTASETRFNGDSESPWVPDNLTKN
                     MVNNHLIHGAEPDDRNILLKIMENGTWNQNQYDSLSSSGKSVLTLLGRPSLDEAKKAI
                     TNLNAHTADFLKKISPSSNIDMLKADVLLMHDLQDRLVPAEESRRLARAVRSNGGEVY
                     HTEFSLFQNSVKVHTTEADETGKLNFIKQAFKLYRHMHKVMSLSG"
     gene            4414..4734
                     /locus_tag="QF838_07955"
     CDS             4414..4734
                     /locus_tag="QF838_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60649.1"
                     /translation="MFLIRRVCRVERKNSWQVAKLLTSICAAYEHETDRGKATIYVGG
                     AGTPAEEFTVCAEWQQETIEANRMPNVPDSVLSDNAELFQLISDYKIEFFEVATDEKL
                     AERS"
     gene            complement(4819..5718)
                     /locus_tag="QF838_07960"
     CDS             complement(4819..5718)
                     /locus_tag="QF838_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013302941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HJO60650.1"
                     /translation="MIPLIKIVRQYERLAVFTLGKFSERNGLKGPGIRILVWPFQTST
                     RIDLREEVIDIPRQTNITSDNAPLDIDFLVFLRPMEHEAQKVVLEVINYHQAVIGMAT
                     TILRAVVGEMTVDEVLSQRERINDALRVKLDEITGRWGIKVTQVEIKEIEPAADIQSA
                     MNRQMAAERLRRAEVTEAEGKRQASITVAEGEKQAEILRAEGQRQSEILAAEGDQQAS
                     VLRAEGFSLALDRIYSVAQNVDTKTLSLQYFETLKEMGKGDSTKFIFPMEFTKLLGPF
                     ASALSVLEDTGPSGDKAQVEEGN"
     gene            complement(5719..>6204)
                     /locus_tag="QF838_07965"
     CDS             complement(5719..>6204)
                     /locus_tag="QF838_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014062.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HJO60651.1"
                     /translation="LPVNWFAMVLIVAALILFVIETQGAGIGLFAIGGTICFGLGGFL
                     LYGDFSDTSLQGNIFSLSMWLLGLVIVLLSVLVLFFILALRDSIARGSPSNYSTLIGS
                     EGTVKGTLNPNGTVLVGSEMWSATSLSNLKIDTGKSVVVSNYDGVRLIVSEKPEDYKE
                     F"
BASE COUNT         1637 a         1380 c         1307 g         1880 t
ORIGIN      
        1 gtggagattt ccggactgta tgctcatcga taccggtcat cggcaccgtc tgcgcacttt
       61 gtttccctgc ctttctcttt ttccattctt accgttgacg gcacattgaa tgtttttaga
      121 tccgactgga atctctgggc ctccttgtaa gtggttcggt ggtatggtcc gtcatcggtt
      181 ggatttaccg ggatgagatt tacgtggcac agtaagtcct tgagaattcg tccaagcttg
      241 ttggcatgca ttgatgtgtc gttttggtct tttaataaga cgtattcgat gaaaatgcgc
      301 cggttagttt tggttacaaa atatttacat gcctcgataa gttcctctat cggataccgt
      361 ttatttactg gcatcgtttc tgatctggtt tcgttatccg gcgcatgtaa ggagactgcg
      421 agatttattt gtatgttttc ctttgcaagt ttctctatac ctggaacaag cccaacagta
      481 gataccgtta tgtgcctagc ccctattcct atgccttttg gatcattaag aacccaaatg
      541 gattccatca tgttttcata attagccagt ggttctccca tccccataaa aacaacgttt
      601 gtaacgccct gtagctctcc tcgagatctc gtaccggctt ggatttcctc tttgtcttcc
      661 gctagggcca atcgttccat agttaaaact tgttctgtga tctctccggc ggttagattc
      721 cttctaaatc cttgctgacc tgtagcgcag aaggtgcatc ctagggcaca tccggcctga
      781 gtggatacgc aaactgtttt ccttcctctt cgatgaccat ctggctcgta acgcattaaa
      841 acagtttcga ttagttcacc gtctggtagc ttaaataaaa ttttatcggt actattgtcc
      901 gcagattttt tcgaaataac tgtagccgcg tttccgatag aataattttg tgccaatatt
      961 cctcgtaggt ctttacttaa gttggtcatt tggccaaagg aagttctacc ttctttgtag
     1021 attgccctcc atacctgttc ggctctatat ttaggttgat caagagcctc tagagaggcc
     1081 agcaggtttg acttagacag ttccagtatt gaaggcaaag tagcctctat agcagatatt
     1141 tttcttccgt tcaccataaa accttcacgg tcgcagattt ttctatttat tcaagtgtat
     1201 caagtactaa tttatatgct tcgctgtttc ctaagtttgt aactgaaact atctctcgtg
     1261 aaatgtctct gattccaacc ccttcatcgg acaatttttg gattagtgtc aaggtcgcac
     1321 ttctatctag gtgttctatc tcaacgtcag atttcgttaa gactaaggtg aattctcctt
     1381 tgggttcgct gaaatgaatt agtgcggcgg caggtttgcc atggaatgtt tcctcgtgta
     1441 ctttagtcat ctctctgcct acaaaaatgc tgcgctcagg gtatagatgt gccaattttt
     1501 ccaaaagatt ttttaaccgt ttgggagact cgaacaatac tatagggact gctaacgcct
     1561 cgtattggct gaggaatttt actaggtctg atttcttccg tggagggaat ccagcgaata
     1621 aaaaggaatc agtagggaag ggacacatag ataggatagt ggtcactgca gaaggtccag
     1681 gaattcccat aacggtaaag cctgcctcat cagcagcctt gactaattgg tatccgggat
     1741 cactaattcc aggggtgcca gcatcggtgg ttagtgcgat atctccccga cctaattcct
     1801 ccaggatttc gggtattttc tgggcgtagt tatctttgtt gaaactagtt tttttagttt
     1861 ttatatcgta ccgagccaat aatttttggg tgactcttgt gtcctctgcc gctattaaag
     1921 tgactcgaga aagaatatca atggctcgga aggtaatgtc cttgagatta cctataggtg
     1981 tgcctaatat gtaaagagta cccattagtg tatccccgtt atcagttact tcaaatccgg
     2041 cggtactgct atttttcaag caatagtgat agttcctttg ggtggctcag catagggtaa
     2101 tgacctgctt ccagttcatg ccatttagca tttaatttct cggctgtccg tttttgatgc
     2161 tcccgtgaag gattggaact taatgtgcac ctaattacag tggcattcca attccgattc
     2221 caaaaattgt ccaactcccc cggggcatct gttgcttcga taggatgcat tgtagccctt
     2281 tctatggccc attttttgag ctcggggtcc aaatcctgga aaaggcgctc ttctaattct
     2341 gcctcggaag ggcctcgggt tagttcagtg atcgtctgcg gaaaggatgg atctctcacc
     2401 actatatcct gaactcgctc tcccggctgt ggggcaagag cgtcgacaaa aaatatctcc
     2461 ttgactctgc tacgagaaag ttctgcgacc ttacaggtga ctaaccctcc tgtactagtc
     2521 cccaccaata tcaaatcggt taaatcttgg tagttcagaa aaccggatac ttcgttcgct
     2581 tggcttttta tcgttattcc tggccttata gagattgaac gctcaccgca accatccagt
     2641 gttggggtgt acacagtatg gcctttcttc actaactctt ttgcggtggg tttccatatc
     2701 catccgcctt ggtaagaacc atgtattaaa acgtaagttg acataagacc ccttcctata
     2761 gagttattgg cgaaagtata ctacccctag gatagggcat acctaatatt tcaaaggtat
     2821 tagaaactga taaatgcgta aatcaatcaa aattcctttc cttgtaattt tccttgtact
     2881 tctgctttta atcacgcctc aggcaagggc ttcttttaag gcagctcttt ttattccgca
     2941 aattttaccg gcgattccaa ttaagccttt ggaatatgtt tccggcaccc cgactaggct
     3001 ggaaataaca tttgagtcct ccaaaggttt ctctgaggct gacatctatt tgccttccgg
     3061 aaatggcact catcctggag tggttttctt tatgggtatt gtcccccctg atagagaaga
     3121 gaaacgtatc ctagctttgg ccgaagggct tgcgaggacc ggtatggtag ttttgatccc
     3181 ctggcttgag actcaagccg aaaacagatt agttgttgat gacatagaaa ccttagtgga
     3241 cgctttcctt tacctgcaag agcatgaaag agtggatggt acgcgagttg gcatgggagg
     3301 gatctgcaca ggagcctcga tggctgttgt tgctgctcag tcaaatcgta ttaatgagga
     3361 agttactttt gttaatttat ttgcgggtta ttatgatgct tttgatctag tgaaatccac
     3421 cgcctcagaa actaggttca atggagacag cgagtcccct tgggttccag ataacctaac
     3481 caaaaatatg gttaataatc atctaattca cggtgccgaa cctgatgata gaaatatttt
     3541 gctcaagatt atggagaatg gtacttggaa tcagaatcag tatgattcat tgagctcaag
     3601 tggtaaatct gtattgacgt tgctaggtag accaagcttg gacgaggcaa aaaaggcaat
     3661 cactaacctg aacgctcaca cagccgattt cctaaaaaaa atttctccca gtagcaatat
     3721 agatatgcta aaagccgacg ttttattaat gcatgatcta caggacagac ttgtgccggc
     3781 tgaggaatcg agaaggctag ccagggccgt caggtctaat ggcggcgagg tttaccatac
     3841 cgaattttcg ttgtttcaaa attcggtgaa agtccataca accgaagcgg atgagactgg
     3901 gaaattaaat ttcatcaaac aagccttcaa actttacagg cacatgcata aggttatgtc
     3961 gttatctggc taatttttaa tttgcctttt tctttatacg aaggacgaat cattccaaac
     4021 gctaccagca caattaatgg taaacatgtt ttgttcatat ccgatataaa tgatggtatc
     4081 gacccaattg ggagcattac gacagcgact accacaaggt atgaaattag cacccactga
     4141 acggtggata aatcagctga gaaatactct gcaatctgag gtattatcac gttggacgaa
     4201 ccacgattca ccgccgttcc gaggatcgaa agggcactca acagaaaggc taaccatttg
     4261 taatgttttg atttgaatga tcgcaaatag ctgaatttcg taacaagtaa tcaaaagcat
     4321 acatccaaac catctcttgg tcttaggcat atacaatgtt gcctcttgtt gttttgtacc
     4381 aactaaagta ttttcaccag atgaggagaa atgatgtttt taataagacg agtttgcagg
     4441 gtcgaacgta agaattcatg gcaggtagcc aaattattaa cgagcatatg cgccgcctat
     4501 gaacacgaaa ccgatagagg caaagctact atttatgtcg gtggggcagg gacccctgcc
     4561 gaagaattca cagtctgcgc agaatggcaa caggaaacaa tagaagcaaa tagaatgcca
     4621 aacgtaccag atagtgttct tagtgataat gccgaattat tccaattaat atcagactac
     4681 aaaatagagt tttttgaagt agctaccgac gagaaactag cagagcggtc ttaacgttgg
     4741 gaaagaggaa tcactccatg gattccactc tcccaaataa cctcaaatta gaggcctagc
     4801 tactttttga tgccctaact aattgccctc ttccacttgt gccttgtcac cggaggggcc
     4861 ggtatcctcc aaaacagaca gtgccgatgc aaaaggccct aggagtttcg tgaattccat
     4921 tgggaagata aactttgtgg aatccccttt tcccatttcc ttcaaagtct caaagtattg
     4981 taagctgagc gttttggtgt caacattttg ggcgacggaa taaattctgt ctaatgctaa
     5041 actgaaacct tcggctctca gaacagaagc ctgctgatct ccttctgcgg caagtatttc
     5101 agattggcgt tgaccttccg ctcgtaatat ttctgcctgt ttttcacctt ctgccactgt
     5161 aatggatgcc tgtcttttac cttcagcttc agtaacttcg gctcttctta acctttccgc
     5221 ggccatctga cgattcattg ctgactgtat atcggcggcg ggctcgattt cttttatttc
     5281 tacctgtgtt actttaatgc cccaacggcc agtaatctca tctagtttaa ctcgcaatgc
     5341 gtcgttgatt cgttcccgtt gggaaagaac ctcatcaaca gtcatttctc cgacgacggc
     5401 ccgtaatatg gtcgtggcca ttccgattac tgcctgatgg taattaataa cctccaagac
     5461 aaccttctgt gcttcgtgtt ccattgggcg aaggaaaact aggaaatcta tgtcaagtgg
     5521 tgcgttatca ctagtaatgt tggtttgacg agggatatcg atgacttctt ctcgtagatc
     5581 gatcctggtg gaagtttgaa acggccagac caaaattcgt attcctggac ccttaaggcc
     5641 atttctttca ctaaatttac ccaatgtaaa gacagctagc ctctcatatt ggcgaacaat
     5701 tttaatgagt gggatcattt aaaactcctt ataatcttca ggtttttcag agacgattaa
     5761 acggacaccg tcataattac taacgacaac tgattttccg gtatctattt tcaagttact
     5821 taaggaagtg gcactccaca tttcagaccc aactagcacc gtaccgttcg gattcaaagt
     5881 tccctttacc gtcccttcac ttcctatcaa ggtactgtag ttacttgggc ttccacgtgc
     5941 tattgaatct cgcaaggcca aaataaaaaa taagactaaa actgataaaa gaacaattac
     6001 taaccccaag agccacatgc tcaagctgaa tatgtttccc tgcaaagaag tatcgctgaa
     6061 atctccatat aaaagaaagc ctccaagacc gaaacatatc gtcccgccta ttgcgaagag
     6121 acctattccg gcaccttggg tttctattac aaaaagtatt agcgccgcta ctatcaaaac
     6181 catggcaaac caattcacgg gtaa
//
LOCUS       DASCNS010000217         5558 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001651573, whole genome shotgun sequence.
ACCESSION   DASCNS010000217 DASCNS010000000
VERSION     DASCNS010000217.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001651573"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="QF838_07970"
     CDS             complement(<1..1214)
                     /locus_tag="QF838_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60652.1"
                     /translation="MVNKDTPPWRNNKLWSLAVAETLSWACLFYVFPALLLRWKESFG
                     WSISQLSIGFTLALIASAITGIIAGRIIDSGHSRKLMTSSVVIGAILLSLLPTVTAIW
                     QFYLIWLLIGCTLAGCLYDPCFSYLTRTFQGNAKNGIIMITLIAGFASTISYPMCTLV
                     SELYSWEITIYLLSGILCLISAPLFWYGTSIRVGTVSNSGEKSTSSVIEIIKPTLSSP
                     VFWGLLVTFAAMSSNQGMIVSQIFPLLESRNISPTSTVFFASCIGPMQVIARLILFAA
                     ESFTKKNIPMMAVCLTSLIFLCLSSLALVLGGASLIIIVVFVMFQGGPYGLFSIVRPI
                     ITADLLGRVNFGLLSSMVGIGSVWGSAFAPGVAGSIADRWSYDTVLLTTSSIAATGLV
                     SLIVTWRLQRKNP"
     gene            complement(1231..1875)
                     /locus_tag="QF838_07975"
     CDS             complement(1231..1875)
                     /locus_tag="QF838_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HJO60653.1"
                     /translation="MTVLFEKLHGPDGALKAISNLDKDIELALETYGPPPDRSLPATF
                     QTLARSIVGQQISGAAATSVWKRMKEAEVSTEQVISKLEPDDMMPLGLSRRKAEYIIG
                     IAKEITTGELDLRSFPQMSGESVQERLVKIRGVGAWTADNYRLFALADLDAWPVADIA
                     LQEGMKILKSLDMRPDVKTMETMGEPWRPFRGVGALILWHIYGSHKRKATIADI"
     gene            1931..2695
                     /locus_tag="QF838_07980"
     CDS             1931..2695
                     /locus_tag="QF838_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015763.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60654.1"
                     /translation="MDSDSGAIAVVILGFLLGLKHATDADHVVAVGTIVNEYGNAFRS
                     LWVGASWGLGHTAPLLILGIVILLLKSAVMDFYEGVAHYFELGVAAMLIFLGLQVYWN
                     LRRRHLHVHQHAHDGGSHMHIHGTHSSQEDPSIEKPHSLFNPGRPFFRLKSFLIGFIH
                     GMAGSAAVMLVLLPTIDTFTTGLLYLILFGVGTVFSMALITILLSVPFVISSNNKRIT
                     NIVNGVAGTASIVFGLLLMSDVAFDTKLTEPFYLPF"
     gene            complement(2724..3950)
                     /locus_tag="QF838_07985"
     CDS             complement(2724..3950)
                     /locus_tag="QF838_07985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60655.1"
                     /translation="MNNINKGPLQGMKVLDWTMWQFGPVSTMMMADLGAEVIKIESLD
                     GDHGRQFGQVSGVASNAYDGVSSHYFEVLNRQKLGIAINLKAKRGQKIIHSLVKESDV
                     FVQNFRQGVAERLGLGWEDLQKINSQLVYGSATGYGPLGGDSNKPAFALTGEARSGSL
                     WWGGPEDDTPYNLHGVADQMAGIMLSYGILSGLLYKERHGIGQKVETSHLGSMMWLGM
                     MRDGIGLLEGQELSRQHRSNMNNPLWNTYKTKDQVWIALSMSQGVRYWPIFCKTIGRE
                     DFITQAEFSTPKSREENKHHVIGELDKIFGSKTFPEWEKILGSSDLIWEKVQSTLDLP
                     KDNQVTLNNYLVPFDHPIIGDSMWHQLPLAFDKTPLSTEKMAPSLGENTEEILIDRLG
                     YSWDDISSLQDEGIIL"
     gene            complement(4036..4770)
                     /locus_tag="QF838_07990"
     CDS             complement(4036..4770)
                     /locus_tag="QF838_07990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60656.1"
                     /translation="MPDMKKTALITGGSRGIGKAIAKDFSKNGIDVVVTGRDKDRLRA
                     TAKELETYGNNAYWIDADLGTDTGVTRIFEFLKEKNLQVNVLVNNAAIIHLSTHLIDF
                     DMENWQEVINVNLIGAVKITKLLLPGMVSQNYGKIINISSIGGRKGAAGRSAYRVTKA
                     GLISFTESLAAEVKSSGIDVNCVCPGSVVTEGYVEAFGHQSIENDHMMDPSEIATLCS
                     FLVSDNSSAVTGATIDAYGPSNPLFR"
     gene            4932..>5558
                     /locus_tag="QF838_07995"
     CDS             4932..>5558
                     /locus_tag="QF838_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60657.1"
                     /translation="MPATKFETTFRRVLADGKHFGDVGQYEEIRGVLSFQIDPENEAN
                     SRITDVRLAPVNQNGFVEFESDVSLILPVDKTKVSGKLLLDVVNRGNRVGLPNFNRGT
                     RPLIDENTPIDVEVDLGDGLLMEKGYVVVACGWQVDAPPFDALITMRGPEALNPDGSR
                     LEGKVYMQLQSPEDTHNFLLSDKGQDFSLQGVWGPRFFTSRGLGPKIFH"
BASE COUNT         1538 a         1198 c         1146 g         1676 t
ORIGIN      
        1 ggattttttc tttgaagcct ccaagttact atcaaactta ccagaccagt ggcagcaata
       61 gaggatgtgg taagtaatac tgtatcgtag ctccatcgat cagctatgct gcctgccaca
      121 ccaggggcga aggctgaccc ccatacactt cctataccga ccattgaaga taataaaccg
      181 aagtttaccc tgccaagtag atcggccgta ataattggtc tcacaatgct gaataaaccg
      241 tagggtcctc cttggaacat tacaaaaacc acaataatta ttaaggaagc cccacctaaa
      301 actagagcaa gactagatag gcataggaaa atcaaacttg tgagacacac tgccatcata
      361 ggtatgtttt ttttggtaaa cgattctgct gcaaaaagaa ttagtctagc aattacttgc
      421 attggaccaa tacatgaagc aaaaaataca gtggaggtgg gtgaaatatt tcgactctcc
      481 aggagaggga agatctggct gactatcatt ccttgatttg aagacattgc ggcaaaagtc
      541 accagcaggc cccaaaatac agggctagac aaggtaggtt tgatgatctc tatcactgaa
      601 gatgttgatt tttccccgga attggataca gtaccaactc tgatagatgt tccgtaccaa
      661 aataaaggag ctgagatcag gcaaagtatt ccagataaaa ggtaaatcgt aatttcccag
      721 ctgtaaagtt cagaaactaa ggtacacata ggatatgaga ttgtgctggc aaaccctgcg
      781 atcaaagtaa tcatgataat gccgtttttt gcatttcctt gaaaggtacg agttagatac
      841 gaaaaacatg ggtcatagag acatccagcc aaggtgcatc caattaaaag ccaaattaaa
      901 taaaattgcc agatcgcagt cacagtgggc aagagtgaaa gtaaaatagc acctattacg
      961 acactagagg tcataagttt tcgagaatgc ccactatcta taatccgccc ggcaatgatt
     1021 ccagttatag cagatgctat aagagccaat gtaaagccaa tggataattg agagatactc
     1081 catccaaatg attctttcca ccgtaacaac aaagctggaa agacatagaa gagacaggcc
     1141 caagaaagag tctcggctac agcaagtgac cataatttat tattacgcca tggcggggta
     1201 tctttattta ccattgggtc ctgctatgag ttagatatcg gctatcgtag cctttctttt
     1261 gtgagaacca tatatatgcc ataggataag tgccccgaca cccctaaaag gtctccatgg
     1321 ttcgcccatg gtttccatgg ttttcacatc tggtctcata tcaagcgatt taagtatttt
     1381 cattccttcc tggagcgcta tatcagctac tggccaggca tctaggtctg caagagcaaa
     1441 aagccgataa ttgtctgccg tccaagcacc gacgccccta atcttaacga gtctttcttg
     1501 cacagattcg ccactcattt gaggaaatga tcttagatct aattctccag tggtgatttc
     1561 cttagctatt cctatgatat attcagcttt tctacgggat aaccccagtg gcatcatatc
     1621 gtctggttcc agtttagata taacttgttc tgtcgagact tctgcctctt tcatacgctt
     1681 ccatacgctg gttgcggcag ctcccgaaat ttgctgaccg acgattgacc tagctaatgt
     1741 ttggaaggta gcaggtaatg aacgatcggg tggggggcca tatgtttcaa gggctagttc
     1801 gatgtcttta tccaaattgg atatggcctt caaggcacca tctgggccgt gtagtttttc
     1861 aaacaaaaca gtcataggaa tgatactaat gtatcatagg ggttggagca aatttcattg
     1921 aggtgttttt ttggatagtg attctggggc aatagctgtg gttattcttg ggtttttact
     1981 tggactcaag catgctaccg atgctgatca tgttgttgct gtgggaacaa tagtaaatga
     2041 gtatggaaat gcgtttcgaa gcctctgggt aggtgcctcg tgggggctcg ggcacacagc
     2101 accactacta atattgggga tagtcatatt acttcttaag tctgccgtaa tggattttta
     2161 tgaaggagtt gcccattatt ttgaacttgg tgtggcagcg atgctgatat tcttgggcct
     2221 ccaggtgtac tggaatttga ggaggcgcca cttacatgtg catcaacatg ctcacgatgg
     2281 tggttctcat atgcatattc atgggactca ttcatcacaa gaagatccct ccattgagaa
     2341 acctcactca ctttttaatc caggcaggcc attttttcgc cttaaatctt ttttaatcgg
     2401 atttatacac ggtatggcag gtagtgcagc cgtgatgctg gttttattgc ctacaataga
     2461 tacctttacc acaggattgc tttatttgat cctttttggt gtaggtacag tattttctat
     2521 ggcgctaatc accattttat tgagtgttcc ttttgtaata agctccaaca ataaaaggat
     2581 taccaatata gtaaatggag tagcaggaac cgccagtata gtttttggtc ttctgttaat
     2641 gtccgatgta gcattcgaca ctaaacttac ggagcctttt taccttccct tttaaattcc
     2701 atcaatgaaa ttaaaagatt aagctacaaa ataatccctt catcctgtaa cgatgatata
     2761 tcgtcccaag aataacccag gcgatcgata aggatttctt ctgtgttttc tcctagagat
     2821 ggtgccattt tttccgtaga taacggagtt ttgtcaaagg ccaatgggag ttgatgccac
     2881 atcgaatcac cgatgattgg atgatcaaaa ggcacaagat aattatttag tgtgacttga
     2941 ttgtctttgg gcaaatcaag ggttgattga actttttccc atatcaaatc agaactcccc
     3001 aatatttttt cccactctgg aaaagtcttt gatccaaata ttttgtccaa ttcaccaatt
     3061 acgtggtgtt tgttttcttc cctcgattta ggggtactga attcagcctg tgtgatgaaa
     3121 tcttcacgtc caatcgtttt acagaaaata ggccaatacc tcaccccttg gctcatggat
     3181 aatgcaatcc agacttgatc tttggttttg taggtattcc acaaggggtt gttcatgtta
     3241 cttctgtgtt gcctactcaa ttcctgacct tcaagtagcc ctattccatc ccgcatcatt
     3301 cccaaccaca tcatgctgcc taggtgagaa gtttcaactt tttgaccgat gccatgacgt
     3361 tctttgtaaa gtagtcccga taatatgccg taactaagca ttatccctgc catctggtct
     3421 gcgacaccat gtaggttgta aggtgtgtca tcttccggtc ctccccacca taaagagcct
     3481 gatctagcct ctccagtcaa ggcaaaggct ggtttattgg aatctcctcc cagcggtcca
     3541 tatccagtag ctgacccgta taccagttga gaatttattt tttgtaaatc ttcccaacct
     3601 aaaccaagtc tttctgcgac accttgtctg aaattctgaa cgaaaacatc ggattccttt
     3661 actagggagt gaataatttt ttgcccacgt tttgccttta aattgatagc tataccaagc
     3721 ttctgcctgt tgagtacctc aaaatagtga gagctgaccc cgtcgtatgc attactcgcc
     3781 accccactta cctgcccaaa ctgtctccca tggtctccat ccaaggactc tattttaata
     3841 acttcggccc ctaagtctgc catcatcatt gttgagacag gtccaaactg ccacatagtc
     3901 caatccagga ctttcattcc ttgaagggga cctttattaa tgttgttcat aagccttgag
     3961 atcttttaag ggcatatttt ttggaggctt tggcaatact aacaggaaca ttcaccaatc
     4021 aaaaatgttg ttaagttatc tgaataatgg gtttgaaggt ccgtatgcat cgatggttgc
     4081 tcctgtgacg gcggaagaat tgtcacttac caaaaatgag cataaagtgg caatttcaga
     4141 tgggtccatc atgtgatcgt tttcaatgga ttgatgacca aatgcctcca catagccttc
     4201 tgtaacaacg ctaccggggc aaacgcagtt gacgtctatc ccagaagatt ttacctctgc
     4261 agccaatgat tctgtaaaac taattaatcc ggccttggtt accctatagg cacttcttcc
     4321 tgctgctccc ttccgtccac ctatcgatga gatattgatg atttttccgt aattttggga
     4381 aaccatgcca ggtagtagaa gctttgttat ttttactgca ccgatcaaat tgacatttat
     4441 aacttcctgc caattctcca tatcgaaatc tatcaaatgt gtagataaat gaataattgc
     4501 agcgttgttc accaggacgt tcacttgaag gttcttttct tttaggaatt caaagattcg
     4561 agtcactcct gtgtcagtgc ctaaatctgc atctatccag taagcattgt taccatatgt
     4621 ttcaagttct tttgccgtgg ctcggagccg gtctttatct ctgccggtta ctacaacgtc
     4681 gatcccattc tttgaaaaat ccttcgctat agctttacca attcccctgg accctcccgt
     4741 tattagggct gtctttttca tatccggcat tattacctcg tttgtgtata cataaatatc
     4801 tatggatttt agccccttac tgtaaagatt gaaaattcat gatttacggt aggccaaaac
     4861 gggaattttg cgaacttttc tcaaatgctg taaaacagtc atctcatttg taatacatat
     4921 aggagtctct tatgccagcc actaaattcg aaacaacctt taggagagtt cttgctgacg
     4981 gaaaacattt cggtgatgtt ggccaatatg aagagattag gggagtttta tcctttcaaa
     5041 tcgaccctga aaatgaggct aattccagaa taactgatgt aagactcgcc ccggtaaacc
     5101 aaaatggatt tgtcgaattt gagtcagatg tttccttaat tcttcctgta gacaagacta
     5161 aagtgtcggg aaaattactg ttggatgtag taaacagagg gaatagggtt gggctgccta
     5221 actttaatag aggaacgaga cctctaatag atgaaaacac tccgatagat gttgaggttg
     5281 atttaggcga tgggctttta atggaaaaag gctatgtagt tgtggcttgt ggttggcagg
     5341 tcgatgcccc tccttttgat gctctcataa ctatgcgagg acctgaagcc ctgaatcctg
     5401 atgggagtcg gctggaaggc aaggtatata tgcaacttca gtctcctgaa gatactcaca
     5461 atttcctgct ttcagacaaa ggccaagatt tttcccttca aggggtctgg ggcccaagat
     5521 ttttcacctc aaggggccta ggacccaaga tttttcac
//
LOCUS       DASCNS010000218         8237 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001685733, whole genome shotgun sequence.
ACCESSION   DASCNS010000218 DASCNS010000000
VERSION     DASCNS010000218.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001685733"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            237..383
                     /locus_tag="QF838_08000"
     CDS             237..383
                     /locus_tag="QF838_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60658.1"
                     /translation="MEELKLPTDGPDPHGLTIRDGQLWYSDADFPTVQGMRGYPEIGV
                     ISYQ"
     gene            487..1845
                     /locus_tag="QF838_08005"
     CDS             487..1845
                     /locus_tag="QF838_08005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60659.1"
                     /translation="MKLVAFLQAQNCTTLPSSWRHPDARIDTYSPEYYQHIARVLEQG
                     KFDIGFFDDRLAMPDMYAGDHSETVKNGIRCTKLDAVTCLMSMAAVTKHLGLGATYST
                     TYYEPFHVARLFQTLDLMTKGRAAWNVVTSVNDNEARNMGREAHLEHDTRYDRADEFC
                     EAVLGHWDSWDDDAIVVDKENNLYAHPDKVRKIDFKGKYLSSRGPFTVPRTPQGRPVV
                     IQAGGSTRGKRFAARWGELIFAGYRNIDVGKLEYSAAKRAAEAAGRNPDHMKIASLMY
                     PIAAATKSEAEDKKAAYELLSNDMDQLLLLSEALNFDFSSKGMDEGFTEEELEGLQGM
                     QAMRDRVVSSGVKNPTPRDFMRITRRGLLSDGHTMVGGPKDIADEMEEWFHSPACDGF
                     VVGPTHQPGAFEDFVKFVVPELQKRGVFRKDYSGSTLRDHLGLDRAAIGSWKTNYDTV
                     ST"
     gene            complement(1866..2798)
                     /locus_tag="QF838_08010"
     CDS             complement(1866..2798)
                     /locus_tag="QF838_08010"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HJO60660.1"
                     /translation="MTNKHASNLAQRLEPGLFTVTAEVTLPDSIEKEALASRAHILEP
                     YCDAINITDSTGAVPHFSNIAAASILIENNIEPIIQFSCRDRNRIATQADVIGAHAMG
                     VRNIFCVTGDGVQTGDHPEAKAVFDLDSVTLLTTINHLKTTGKFLSGRKLDSKPDLFL
                     GAAVNPFVPPFEQRALRLLKKHRAGASFFQSQYCFDISRFESFLLLLLELTNGSPPPV
                     LVGVGPLRSARFARWLRENVPGVAIPDHIINQIEKYPEKEQQKAGIEACVKIVEGVKN
                     LTGVAGIHIMAHNWESSVSEIVEATGLTKTGRNN"
     gene            complement(2795..3211)
                     /locus_tag="QF838_08015"
     CDS             complement(2795..3211)
                     /locus_tag="QF838_08015"
                     /inference="COORDINATES: protein motif:HMM:NF023646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HJO60661.1"
                     /translation="MNKVRLLEFTYLFCLKILSRLVPILSEKRPFIGFMVASEKAFKK
                     PLFGCQMCGQCILHYTGMVCPMGCPKNLRNGPCGGVRGDGNCEIKPNMKCTWVRAWDN
                     STQMMVFTESIEEIQTELDHSLSGTSAWINELGGGE"
     gene            complement(3332..4315)
                     /locus_tag="QF838_08020"
     CDS             complement(3332..4315)
                     /locus_tag="QF838_08020"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO60662.1"
                     /translation="MVTFGSGDYKYGGVENFFKRPKGWPFVEVADVAVDKDDNVYVFN
                     RGPYAAIMIFDKSGEYLGGWGKIGFDFIVPHGVSIGPDGSVYTSDTGDHTVRKWTKDG
                     KLLMTLGRPLINAPEQSGIPFNRPTHFTVASNGDLYASDGYGNSHIHCFDSKGNLKFS
                     WGGHGSGPGEFDTIHSVYVDIDDNDKIYATDRYNNRIQHFSITGEFLGEWTNVHLPND
                     VRKGPDGNFYVAELDHRVSILDSSGTLLARWGDVDANVDDSETGSGLPDSPSRNPMIM
                     GKVKHEPGAGLFGAPHGIAVDSEGSFYVSEVSETFLGIDRGDRSVQKFVRV"
     gene            complement(4332..4407)
                     /locus_tag="QF838_08025"
     tRNA            complement(4332..4407)
                     /locus_tag="QF838_08025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4372..4374),aa:Arg,seq:tcg)
     gene            complement(4593..4928)
                     /locus_tag="QF838_08030"
     CDS             complement(4593..4928)
                     /locus_tag="QF838_08030"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJO60663.1"
                     /translation="MTIEITSEMKELINNALPDKYPCIVGTSSSDGLPNVSYKGSVMV
                     FSATELAFWERTRKGGFAQLTDNPNIVVMYRNTELRKSWRFYGEVAIYDSGEIKNQVL
                     EKTVQPELD"
     gene            5151..5603
                     /locus_tag="QF838_08035"
     CDS             5151..5603
                     /locus_tag="QF838_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60664.1"
                     /translation="MITLLQIFKCWLGGQIKLGEKKGELSGTTANKILPAENGSPIIE
                     TTGAGAGTLLGVEGMFQATYQARVNADGTIYGECPNSGVFMTAEGIATFRANGAGAFT
                     EDGGAKFRGTAYFSAAFPSLAGLNGMCLIYEWDVDGEGNATWNLWEWK"
     gene            5784..6554
                     /locus_tag="QF838_08040"
     CDS             5784..6554
                     /locus_tag="QF838_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60665.1"
                     /translation="MPVANNSGTHINYEITGDGPPLILQHGLTSKLDKWNWYGYVDEL
                     KRSFRVISVDARGHGKSDKPYDASLYDRKIMASDIVSVMDRENIDMAHYMGYSMGGSI
                     GFGLAEAFPQRFYSLIIGGMHPYPLGDLELEPGVKALDFMLESLEHGMDVYVGGLEPV
                     PDERELNHLLSNDVKAITAATLALRDRPDISGVLPTMTMPCLVYCGDQDGLYPGTERC
                     VKDMPNVTWISLPGLNHGDVMERSDALLPHVLNFLTDD"
     gene            6580..7770
                     /locus_tag="QF838_08045"
     CDS             6580..7770
                     /locus_tag="QF838_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HJO60666.1"
                     /translation="MSTPLLFQPLTIRSITLKNRVVVAPMHQYAAVKGYPTDWHLMNA
                     GRFAAGGAGLVFIESTKVERRGCGTAGDMGLWDDSYTAHFKRLADFIRKQNSVPAIQL
                     GHSGRKARRFRPWEGGAPLTPEGALDAGVDDWDDWELVGPSPIGSSETEPIPRALSFG
                     EIQKTVENWGEAARRANEAGFEVLEIHAAHGYLLHQFLSPKANQRMDEYGGTESKRMR
                     LPLEVAEEVRANWPEDKPLFMRLSVEDDAGWGPEESVRFSRLAKEKGIDVIDCSSGGM
                     RGSPVISAGPVGYGYQVPYAEKIRKETDMMSMAVGLIVHAEQAEEILQSGEADLIAIA
                     REFLHNPNWALDAALKLGVEDAFKVTPPAYDYWLEKRSSSVKGVVPSTYFPGSDNGSF
                     QREV"
     gene            7800..>8237
                     /locus_tag="QF838_08050"
     CDS             7800..>8237
                     /locus_tag="QF838_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HJO60667.1"
                     /translation="MKYLTEDQIRDLKAIDTPTVANAVETHDARLNTEGFMGWNIKCQ
                     FPDLGVMVGQAVTATLNTTTTGKEKSRSGWHDCLRTIDAMPVPAVIVAKDIGPRPNHG
                     CHFGDGMATVAKKVGAIGLVTDGGVRDVETVYEMGFQMFSVGLV"
BASE COUNT         2231 a         1701 c         2015 g         2290 t
ORIGIN      
        1 cgtagtatta agactactga gatatatgct aactgggtta ggatggcaat atttggataa
       61 cagccttcaa tccaaagtct ctgatcctga ttgacggtac ttcatttgag attattcgga
      121 agttttcctg tgatctcaat gtactgcatg ggttagcttt ggatggtgaa ggtatttggt
      181 gctctgatag ggcagagaaa ttggtagtca aatttcataa gaagacaggg gaaaagatgg
      241 aagaacttaa gcttcccact gatggcccgg acccccatgg actgactatt agagacgggc
      301 agttgtggta ttcggatgcc gattttccta cggtccaagg aatgcggggt tacccagaga
      361 taggggtgat aagctaccaa taattactgt gctaacttga tatctcaggg ttggatataa
      421 ttcagagaat ttgggattga ttcggaaggg tgaaaatgat atgggagtaa tagtgttatg
      481 cgccaaatga aattagtagc ttttttgcaa gctcaaaatt gcactacact accctcttcg
      541 tggcgtcatc cggacgcaag aatcgatacc tattccccgg aatactacca gcacatagct
      601 cgtgttcttg aacaaggaaa atttgatata ggcttttttg atgatcggtt ggctatgcct
      661 gacatgtatg caggagacca ttccgaaact gtgaaaaatg gtattagatg tactaagctt
      721 gatgcagtca cctgcctgat gagcatggct gctgttacta aacatttagg cctgggagca
      781 acttactcca ccacctatta cgaacctttc cacgtagctc ggttgtttca gactcttgat
      841 ttaatgacaa aaggtcgtgc tgcatggaat gttgtaactt cggtcaacga taatgaggca
      901 cgtaatatgg ggcgtgaagc ccacctcgaa cacgatacaa gatatgacag agctgatgag
      961 ttttgtgagg cagttttggg ccattgggac tcctgggatg atgatgcgat agttgtagat
     1021 aaagaaaata atctttacgc gcaccctgat aaggttcgca agatagactt caaaggcaaa
     1081 tacctgagtt cacgtggtcc tttcacagtt ccgagaaccc ctcaaggacg gcctgttgtc
     1141 atacaggcag gcggtagtac tcggggcaaa cgatttgctg cacgttgggg ggaattgatt
     1201 tttgcgggct atcgcaatat tgatgttggt aagcttgaat attcagcagc aaaaagagcg
     1261 gccgaggcgg cgggacgtaa ccctgaccat atgaaaattg catctctgat gtatcccata
     1321 gcagctgcaa caaaatcaga ggctgaggac aagaaggccg cctatgagct gctttccaat
     1381 gatatggatc aactgttatt gctgtctgag gctctcaatt tcgatttttc ttccaaagga
     1441 atggatgaag gctttaccga agaggaattg gagggcctcc aagggatgca ggccatgagg
     1501 gaccgggtcg tatcgtcggg agttaaaaac ccgacgccca gggattttat gaggataact
     1561 aggagaggtt tgctcagcga tggccacacc atggtcggtg gtcctaaaga tattgcggat
     1621 gaaatggaag aatggttcca ttctcctgcc tgtgacgggt ttgttgtggg acccacacac
     1681 caaccaggag cattcgaaga ctttgtcaaa tttgtagttc cagaactgca gaagagaggg
     1741 gttttcagga aggattatag cggcagcaca ttgagggatc accttggctt ggatagagcg
     1801 gctatcggct catggaagac aaattatgac acggtatcca cataagtaga cgttataaaa
     1861 ttcggttagt tatttcgccc agttttcgta agacctgttg cctcgacaat ctcagacact
     1921 gatgattccc agttgtgagc cattatatgg atcccggcta caccggtgag atttttaaca
     1981 ccttccacta ttttcacgca tgcctcgatg ccagccttct gttgctcttt ttcggggtat
     2041 ttttcaattt ggttaattat gtggtccggt atagcgactc caggtacatt ttctctcaac
     2101 cagcgagcaa aacgggcaga tctgagtggt ccgacaccaa ccaatacagg gggaggtgac
     2161 ccgttggtca attccagaag cagtagaagg aaagattcga atctgcttat gtcgaaacag
     2221 tattgggatt gaaaaaaaga ggctccggcc ctatgttttt tgagtaatct caaagcgcgc
     2281 tgttcaaagg gagggacaaa tggattaacg gccgctccta aaaagagatc aggtttggaa
     2341 tctaatttcc taccactcag aaatttacct gttgtcttta agtgattgat tgtagtcaat
     2401 aaagtaactg aatcgaggtc aaacacagct tttgcctcag ggtgatcacc tgtttgaaca
     2461 ccgtctccag ttacgcagaa aatgtttcga acacccatgg catgcgctcc gatcacgtcg
     2521 gcctgcgtag caattctgtt tctatctctg catgaaaatt gaattatggg ttctatgttg
     2581 ttttcgatga gtatcgaggc tgctgcaatg tttgaaaaat ggggcactgc cccagtggaa
     2641 tctgtgatgt taattgcatc gcaatatggt tcaagtatat gggctcgaga ggctagggct
     2701 tccttttcaa ttgagtcagg taatgttacc tctgcagtca cagtaaaaag tcctggctct
     2761 aacctttggg ccaaattcga agcatgttta ttcgtcattc ccccccccct aattcgttaa
     2821 tccacgctga ggttccagat aaactgtgat cgagctctgt ttgtatttcc tcaatggact
     2881 ctgtgaaaac catcatttgg gtcgaattat cccaagctct gacccatgta catttcatgt
     2941 tgggctttat ttcgcaatta ccatcgcccc ttacaccacc gcaaggaccg tttctgagat
     3001 tctttgggca tcccattggg caaaccatcc ctgtatagtg aagaatgcat tggccgcaca
     3061 tctgacaccc aaacaagggc tttttgaaag ccttttcact tgcgaccata aagcctatga
     3121 acggtctttt ctctgataaa ataggtacta gtcgagataa tatttttaag caaaataggt
     3181 atgtaaattc aagtagccgg accttattca taagtcgatt ttacactcta ttcgaatatg
     3241 aatcagcatc aattaaggac aattaactag atagcgtagg aattcgggtc ggttaggagc
     3301 gggaattagg gattaagcat aagtttatgg tctaaaccct cacaaatttt tgaacggagc
     3361 gatcaccccg gtctattcct agaaaggttt cactgacctc cgatacgtag aaactgcctt
     3421 ccgagtcgac tgcgatgcca tgaggtgcgc caaataaacc ggcaccgggc tcgtgtttta
     3481 ccttacccat aatcatgggg tttctacttg gagaatcggg taagccagat cctgtctcag
     3541 agtcatctac atttgcatcc acgtctcccc atctggcaag tagagtgcca gagctatcta
     3601 agatgcttac gcggtgatcg agttctgcta catagaaatt gccatctggc ccttttcgta
     3661 cgtcatttgg caaatgtacg ttggtccatt ctccaagaaa ttcacctgtt atggagaaat
     3721 gttggattct attgttatac ctgtcagtgg cataaatttt gtcattgtca tcgatgtcaa
     3781 cgtacacact atggatagtg tcgaattcac ccggaccact gccgtgtcca ccccatgaga
     3841 atttcaggtt ccctttagaa tcaaagcaat gaatatgtga attcccatag ccgtccgatg
     3901 catatagatc gccattagag gctactgtaa aatgagttgg cctattgaat gggataccac
     3961 tctgttccgg ggcattgatt aaaggtcgtc ccagggtcat taatagcttg ccgtctttcg
     4021 tccattttct aactgtgtgg tcacctgtat cacttgtgta tacggaacca tcaggtccta
     4081 tgcttacacc atgcggaaca atgaaatcga atcctatctt tccccagccc ccaaggtatt
     4141 cacccgattt gtcgaaaatc ataatcgcag cgtatgggcc tctgttgaaa acatatacgt
     4201 tgtcatcttt atcaacggct acgtctgcga cttccacgaa cggccatccc tttggtctct
     4261 taaagaaatt ttccacacct ccgtatttgt aatcaccact gccgaatgtg accatgaaaa
     4321 gttcctttag ctggtacgcc tggagggatt cgaacccccg acacctggtt ccgaagacca
     4381 gtgctctatc cgctgagcta caggcgcacg atatggttat aggaacagaa ttgtatcaaa
     4441 taggagagaa attggttggg aagtatgttt gtagatgttg gagttgaaga aactttttaa
     4501 cactatccag atgcttcctg aactatttcc ccaccaagag tggtgatttt ggtcacctct
     4561 attccgaccg caactcccaa tctatctggg tctcaatcaa gttcaggctg aacggttttt
     4621 tcgagcacct ggttctttat ttctcccgag tcgtatatgg ccacttcccc ataaaatctc
     4681 caggattttc ttagctcagt atttcgatac ataacgacta tatttgggtt atcggttaat
     4741 tgagcaaagc cgccttttcg ggttcgttcc caaaaggcca gctcagttgc cgaaaatacc
     4801 atgacgctcc ccttgtagga tacgttgggg agaccatctg aagaagaagt gccaactatg
     4861 caggggtatt tgtcgggcaa agcgttgttg atgagttctt tcatttcgga tgtgatttct
     4921 attgtcactg gaccttcctt atgtaggttt aagccatatt tattacaaaa gcggattata
     4981 aactcttcac caaaggctcg ctttttttgc tgtgctgtca tagacctttc aaggaatgtc
     5041 ggtacccgat ttgggtaatc accaaaccaa atatagtagg cactctgagg tcaatacaat
     5101 ttcatttacc gtattcagct ctattactac ttaacgactc ttactccttc ttgataacat
     5161 tgcttcaaat atttaaatgt tggctaggag gtcaaattaa gttaggagag aaaaagggtg
     5221 aactgtcagg gactacggca aacaaaatat tgccggctga gaatggttca ccaataattg
     5281 aaacaacagg agccggtgct ggaactttgc tgggagtgga aggtatgttc caggcaactt
     5341 atcaggctcg tgtaaatgcc gatggaacca tttacggtga gtgcccgaat tccggtgtgt
     5401 ttatgactgc ggaaggtatt gctacctttc gcgcaaacgg cgcaggagct ttcaccgaag
     5461 acggaggggc taaattcaga ggaactgctt acttctctgc ggcttttcct tcccttgcag
     5521 gattaaatgg gatgtgttta atatatgaat gggatgttga cggtgaaggg aatgcaacct
     5581 ggaacctttg ggagtggaaa tagtaatgta gacctttata agagaagctt gaaatagggt
     5641 ttcccttata ttttttgttc ttttaatgga ttttgttcta tgcaaccgtt cttaatcaga
     5701 gcttaccatt aatctgactt cggaatatat aatttccgac agtaaaatgc ttctagatat
     5761 ccactaaagg aggtcctctt gatatgcctg ttgcgaataa ctcaggaacc cacatcaatt
     5821 atgagattac cggtgatggc ccacctttaa tccttcaaca cggtctgact agtaagttgg
     5881 ataaatggaa ttggtatggg tatgtggatg aattaaaaag aagttttcga gtaatttcgg
     5941 ttgatgctcg tgggcacgga aaaagcgata aaccatatga tgcttcttta tacgaccgaa
     6001 aaataatggc ttccgatatt gtttctgtta tggacagaga aaacatagat atggcccact
     6061 atatgggtta ctcaatggga ggcagtatcg ggtttgggtt agcagaagct tttccgcaaa
     6121 ggttttactc attaattatc gggggaatgc atccgtaccc tcttggtgac ttggaactcg
     6181 aaccaggagt gaaggctctg gatttcatgc tggaatccct tgaacatgga atggacgttt
     6241 acgttggtgg gttggaaccg gtccccgatg aaagagagct aaatcacttg ctatccaacg
     6301 atgttaaggc gataacagca gctactttgg cactcagaga ccgcccagat atatcaggag
     6361 tccttcccac aatgacgatg ccatgccttg tatattgtgg cgatcaagat ggtttgtacc
     6421 caggtaccga gcggtgtgtt aaagacatgc cgaatgtgac ttggatatca ttgccgggcc
     6481 ttaaccatgg ggatgtcatg gaaagaagcg acgctttgct tccacatgtt ttaaactttc
     6541 ttactgatga ttagtgtgta aacaaataca ggagaattga tgtcgacccc tttattgttc
     6601 caacctctaa ctatacggag tatcacctta aaaaaccgag ttgttgtggc tcccatgcac
     6661 caatatgctg cggtaaaggg ctatcctact gattggcatc ttatgaatgc cggccggttc
     6721 gctgctggtg gagcagggct cgtctttata gaatctacca aagtagagag acgcgggtgt
     6781 ggcactgctg gggacatggg cctttgggac gacagctaca cagcccattt caaaaggctc
     6841 gcagatttta ttcgtaagca aaattcagtt cctgcaatcc agcttggcca tagtggaaga
     6901 aaggctcgtc gctttagacc ctgggaaggc ggggctcctc tgacacctga aggagcactt
     6961 gatgcagggg tggatgactg ggacgactgg gaacttgttg gcccaagtcc tatcggttca
     7021 agcgaaactg aacctattcc acgggctttg tccttcggag aaatacagaa gacagtagaa
     7081 aactggggtg aggcagccag aagggcgaat gaggcagggt ttgaagtatt agagatacac
     7141 gctgctcacg ggtacctact gcaccagttt ctatccccga aagcaaatca aaggatggat
     7201 gaatacggag gtacggaatc aaaacggatg cgtcttcctt tggaagtggc ggaggaagtg
     7261 agggccaatt ggcctgaaga caaaccactg ttcatgcgct tatcggtgga agatgacgca
     7321 ggttggggcc cagaagaaag tgttcgcttc tcgagattgg cgaaagaaaa aggaattgat
     7381 gtaattgact gctcttctgg aggcatgcgt gggagcccgg ttataagtgc aggtcctgtt
     7441 ggatatggat atcaagttcc ctatgctgaa aaaatacgta aagagacaga tatgatgagt
     7501 atggcagttg gtctgattgt tcatgctgag caggcagaag agattcttca gtcaggtgag
     7561 gcagatttaa tagcgatagc tagagagttt ctacataatc caaattgggc acttgatgct
     7621 gctttgaaac ttggagtgga ggacgctttc aaagttactc ctccggccta tgattactgg
     7681 ctcgagaaaa ggtctagttc agttaaaggg gtagttcctt cgacctattt tccaggctca
     7741 gataatggaa gttttcaaag ggaagtttag tatagggacc cactcaataa aggagaccaa
     7801 tgaaatattt aacagaagac caaataaggg acctaaaggc tatagacacc ccgactgtgg
     7861 caaatgctgt cgaaacccat gacgctaggt taaatacaga ggggtttatg ggttggaata
     7921 taaagtgcca atttccggac cttggagtca tggtgggtca ggctgttacg gctactttga
     7981 acacaacaac gacaggcaag gagaaaagta gatcaggttg gcatgattgt ttgagaacta
     8041 tcgatgcgat gccggtgcct gctgtgattg ttgcaaaaga tattggacct agacctaatc
     8101 atggctgtca ttttggagat ggtatggcca cagttgcgaa aaaagttgga gcaattggac
     8161 ttgtgactga cgggggagtc cgagacgttg aaactgtata tgaaatgggc ttccagatgt
     8221 tttctgttgg actagtg
//
LOCUS       DASCNS010000219         1540 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001703467, whole genome shotgun sequence.
ACCESSION   DASCNS010000219 DASCNS010000000
VERSION     DASCNS010000219.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001703467"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            49..606
                     /locus_tag="QF838_08055"
     CDS             49..606
                     /locus_tag="QF838_08055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJO60668.1"
                     /translation="MAPNFTLEEGKDYFATIDIFEGGEIKIDLLEELAPVTVNNFVFL
                     SEQGYYDWVTFHRVIPDFMAQGGDPSGTGRGGPGYYIDNEFHPDARHDSAGVVSMANA
                     GLLPDGQGTNGSQFFITFIKTPTLDGINSDGTLKDCSQGSCHSVFGRVVEGMDVLANI
                     IPRDPATANLPGDVIRTIRITFVPK"
     gene            complement(609..1208)
                     /locus_tag="QF838_08060"
     CDS             complement(609..1208)
                     /locus_tag="QF838_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024812.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HJO60669.1"
                     /translation="MAVIRLANPKRDARQVAAIYYPYVMNTPITFETNPPDGTAMQRR
                     MKKVSEIYTSLVCEHETEIMGYSYGSKFRDRSAYDWIVETTVYVREAAHGLGIGKALY
                     ASLLECLKLQGFTSAYGVIALPNDPSVSLHEHFGFTKDYLMTNAGYRDGQWYDVGFWR
                     LKLSDTSENTLPIIPVDELRSMNYFKNSVAFGEQFLKLS"
     gene            complement(1236..>1540)
                     /locus_tag="QF838_08065"
     CDS             complement(1236..>1540)
                     /locus_tag="QF838_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60670.1"
                     /translation="RDIRASSGIQANSAVTPLELNRDPEDVAPMVAWLASDEAKDVNG
                     HVFHCQGGQISLMSNPTEERTLRKNGRWTVEEIDAMFSGTLGMDMANPAPPQVPKE"
BASE COUNT          413 a          320 c          331 g          476 t
ORIGIN      
        1 ttatctaaac cagatttacc aggtactcca gaagtaatga tttgggatat ggcccccaat
       61 tttaccttag aggagggaaa agattatttt gccactattg atatatttga aggaggtgaa
      121 ataaagattg atcttctgga agaactagct ccagtaacag tgaataattt tgtttttctc
      181 tccgagcagg gatattacga ttgggttacc tttcatagag taatcccaga ttttatggcc
      241 caaggtggtg atccttctgg taccggtaga ggaggtcctg ggtattatat cgataatgaa
      301 tttcaccctg atgccagaca tgattctgca ggggtagtgt cgatggcgaa tgcaggtctt
      361 ttgccagatg gacaaggaac gaatggaagt caattcttca tcacattcat aaagacgcct
      421 actttggatg gaataaattc ggatggaact ttgaaagatt gtagtcaagg ttcttgtcat
      481 tcagtatttg gaagagttgt agaaggcatg gatgtgttag ctaacataat tccccgagat
      541 cctgcgaccg ctaatttacc tggtgatgtt attcgtacga ttcgaataac atttgttccg
      601 aaataatttt aggaaagttt gaggaattgt tcaccaaagg caaccgaatt tttgaagtag
      661 ttcatacttc tcaattcatc aacgggtatt atcggcaatg tattttctga ggtatcacta
      721 agtttaagtc tccaaaatcc aacgtcgtac cactggccat ccctatatcc tgcgttagtc
      781 atcaaataat ctttggtaaa tccaaaatgt tcatgaagcg aaacacttgg atcgtttgga
      841 agagcaataa ctccataggc ggaggtgaat ccttgtagtt tcagacattc caatagtgag
      901 gcgtagagag ctttcccaat tcctaaaccg tgggctgctt ctctaacata tactgttgtt
      961 tccactatcc aatcgtaagc acttctgtcg cggaatttac ttccatagga ataacccatt
     1021 atttcggttt catgctcaca aacgagtgaa gtgtatattt ccgacacctt tttcattcgc
     1081 ctttgcattg cggttccgtc aggagggttc gtttcaaaag taattggagt attcattaca
     1141 tacggataat agatggccgc cacctgacga gcgtcccttt taggatttgc tagtcggatg
     1201 acggccattt ttaattaggt atctctattg tagcgctatt cctttggcac ttgcggagga
     1261 gccgggttag ccatatccat ccctagtgtc cccgagaaca ttgcatctat ttcttcaaca
     1321 gtccatctac cattcttcct taaggttcgt tcttccgtag ggttactcat taaagagatt
     1381 tgtcccccct gacaatggaa gacatgacca tttacatcct tggcttcatc tgaggccagc
     1441 catgctacca tgggagcaac atcttcaggg tcacggttta actccaaagg agtcactgca
     1501 gagttcgcct gaattccgct tgaagctctg atatccctgg
//
LOCUS       DASCNS010000220         1068 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001716790, whole genome shotgun sequence.
ACCESSION   DASCNS010000220 DASCNS010000000
VERSION     DASCNS010000220.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001716790"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="QF838_08070"
     CDS             <1..210
                     /locus_tag="QF838_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO60671.1"
                     /translation="INTSDLLFKNGSIIGSTGASRPHIQTAINMVRDGNVVPVIGGMH
                     PFHDIQEAFHLMKNKASVGRLVLIP"
     gene            complement(223..>1068)
                     /gene="secF"
                     /locus_tag="QF838_08075"
     CDS             complement(223..>1068)
                     /gene="secF"
                     /locus_tag="QF838_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HJO60672.1"
                     /translation="GLNPGIDFTGGSSITIQFPEGSGANQKAIRERLQAIGYPESTVQ
                     NLGNSVIDEERYELFFLRTKTLDETKKDLLVTTLNNQFSPEQTNTLLSFDLVSAVIAQ
                     ETVINACWAVLAAALGIFFYVWWAFRKVPRPIRYGLAAIVALVHDTLIVVGIFAILGY
                     FLDTEVNTMFLIALLTVIGYSVNDTIVVFDKIRENVLTYTNRPFPEVVNLSISETMGR
                     SLNTSFTLLVTLLALLLFGGATIREFLYVLIIGVVVGTYSSIAIATQVLVSWDQKTIG
                     KLISS"
BASE COUNT          314 a          215 c          217 g          322 t
ORIGIN      
        1 attaacactt ccgatctact gtttaaaaat ggaagcatta ttggttctac gggagctagt
       61 aggccgcata tacaaactgc gataaatatg gtcagggatg gaaacgtggt tccagtgata
      121 ggggggatgc atccttttca cgatattcaa gaggctttcc atctcatgaa aaataaagct
      181 agtgttggta gattagtctt aattccttaa aaaaatatca gatcaagagg atatcaattt
      241 tccgattgtt ttttggtccc aggaaactag aacttgagta gcaattgcta tggaactgta
      301 agtccctaca actactccta tgattaaaac atacaaaaat tctcgtattg tggctccacc
      361 gaatagcaaa agggccaata atgtgaccaa aagcgtgaaa cttgtattga gtgatcttcc
      421 cattgtttct gatatgctga gattcaccac ctcagggaag ggcctgttag tataggtcaa
      481 tacattttct ctgattttgt caaacactac gatggtgtca ttgacgctat atcctattac
      541 cgtcaggagg gctattagga acatagtgtt gacttctgtg tctaggaaat atcctaatat
      601 tgcaaaaata cctaccacga ttaatgtgtc gtggaccaaa gcaacaatcg ctgcaaggcc
      661 atacctaatg ggtcgtggga cttttctgaa tgcccaccat acgtagaaaa atattcctaa
      721 cgctgctgct aaaaccgccc aacaggcatt gattactgtt tcctgtgcta tcaccgcgga
      781 aaccaaatca aaactaagta gcgtatttgt ttgctcaggg gaaaattgat tgtttaaggt
      841 agtcactagg agatcttttt ttgtctcatc aagcgttttg gtgcgtaaga agaaaagctc
      901 gtatctttcc tcgtcgataa ctgaattacc taaattttgt accgtagatt ctggatatcc
      961 gatagcttgt aacctttccc taatcgcctt ttgattggct cctgagcctt ccgggaattg
     1021 aattgtaata ctagatccac ctgtgaaatc aatgcctgga ttcagccc
//
LOCUS       DASCNS010000221         2243 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001727342, whole genome shotgun sequence.
ACCESSION   DASCNS010000221 DASCNS010000000
VERSION     DASCNS010000221.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001727342"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="QF838_08080"
     CDS             <1..431
                     /locus_tag="QF838_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dimethylmenaquinone methyltransferase"
                     /protein_id="HJO60673.1"
                     /translation="ALPVPAVIVENDIGLRPNHGCHFGDGMATVANTVGAIGLVTDGG
                     VRDVETVYEMGFQMFSVGLVPAHGNFGLDETNVPVEVGGVLVNVGDVVHADMNGVVVF
                     PVDLVDHVISEAKKVTARESEMMDWVNSDGFSLDKFIEGR"
     gene            complement(438..1211)
                     /locus_tag="QF838_08085"
     CDS             complement(438..1211)
                     /locus_tag="QF838_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60674.1"
                     /translation="MPFANNNGIKIHYHTKGEGPHIVLLHGFMGDIGSWEKLGYPDTL
                     APNYTVVIIDARGHGKSDKPYDPTAYLDKTIATDVIAVLDDINVGKSHYFGFSMGGRV
                     GFELAAYHSERFLSFILGAQTPGSRNEHGNESDRKRIALFEKGPEALKRVMSRSKKEY
                     ESYLEPTLNGDLKAYAAKTKANAMREDISEHLVGLTVPCLVYAGESDSLAHESAKEHS
                     QKMPTARFVSLPGLNHMESIARTDMVIPMVKSFLNEVST"
     gene            complement(1421..>2243)
                     /locus_tag="QF838_08090"
     CDS             complement(1421..>2243)
                     /locus_tag="QF838_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60675.1"
                     /translation="SSAELWAHQTYGPLKEYIPWEIHYRKGSILQRELERIVEEKIQI
                     TNENDNDRRDTDKKYFEDRRQTQLSKDLKDGDVFTDLKVMSTPGHSPDQICLVLDDLI
                     FTGDHILPEITPHPTSKMVFRDAISKNLPEFLKDPSGLYGLGAYLNSLGRVIELGDSY
                     TVLPAHRLYNKSHFNWHGVERAGDILKHHEKRLDQMLKKLQGKTSTLEETTKGIFDRS
                     KLLGSNLYAAMSEIVAHLELLEDTEDILIDESGQISMQGDGTNYKNYISTLLDPK"
BASE COUNT          568 a          446 c          495 g          734 t
ORIGIN      
        1 atgcgctgcc ggtgcctgct gtgattgttg aaaatgatat tggacttaga cctaatcatg
       61 gctgtcattt tggtgatggt atggccacag ttgcgaatac agttggagca attggacttg
      121 tgactgacgg gggagtccga gacgttgaaa ctgtatatga aatgggcttc cagatgtttt
      181 ctgttggact agtgccggct cacgggaatt ttggtcttga cgaaacaaat gtgcccgtgg
      241 aagtcggagg cgtcttagta aatgtagggg acgtagttca tgccgatatg aacggggtgg
      301 tcgtctttcc agtagatttg gttgatcatg ttatatccga agcaaaaaag gttacggcgc
      361 gggaatccga aatgatggac tgggtaaaca gtgatggatt cagcctagat aaattcattg
      421 aaggtcggtg attttcctca cgttgaaact tcgttcaaaa atgatttgac cattggaatc
      481 accatatccg ttcttgctat tgattccata tggttcaatc caggcaagct gacaaaacgc
      541 gctgtcggca tcttttggga gtgttccttg gctgattcat gcgcaagtga gtcagattca
      601 cctgcataaa ctagacaagg cacagtaagc ccgacgagat gttccgatat atcttctctc
      661 attgcgtttg ccttggtttt ggcagcgtag gctttgagat caccatttaa tgtaggttcg
      721 agatagcttt catactcttt cttggatcga gacattactc gttttaaagc ctccggccct
      781 ttttcaaaga gggctattct ttttctgtcc gattcgttcc cgtgttcatt cctacttcca
      841 ggtgtttggg ctcctaaaat aaaggagaga aatctttcag aatggtatgc agccagctcg
      901 aatccgaccc tacctcccat ggagaatccg aaatagtggg atttacctac gttgatatcg
      961 tctagaacgg ctataacgtc agttgcaatt gtcttatcga gatatgctgt tgggtcataa
     1021 ggtttatcac ttttgccgtg tccccgagcg tcaattatca ccacagtgta atttggtgca
     1081 agtgtatcgg gatatcccaa tttctcccat gatccaatgt ctcccataaa accgtgcagt
     1141 agtacgatgt ggggtccctc tccttttgta tgatagtgaa ttttgatccc attattgtta
     1201 gcgaaaggca taattagtca ccgttatata ttgagaataa attattgttg agttttgctt
     1261 ataagcatga ctgtactgac ctgtataaag ctcttgaaag acctccgtga taatttgaaa
     1321 ttgtccgcct atttaataat tgttaatccc ttgtttgacg gtatatagaa atatatattt
     1381 tcaaatattt gagtgccatt aatctttaga gatatattga tcattttgga tctaagagcg
     1441 tggatatata atttttataa ttggttccgt caccctgcat agatatttgc ccagactcat
     1501 ctatcaatat gtcctctgtg tcctcaagca attcgaggtg ggcgactatt tcagacattg
     1561 ctgcataaag gttagagccc agaagtttac tccggtcaaa aatgcccttg gtggtttctt
     1621 ctagagtaga ggttttaccc tgtaacttct ttagcatttg gtctagtctt ttttcatgat
     1681 gctttagaat atcccctgcc ctctctaccc cgtgccaatt gaaatgagat ttattataaa
     1741 gacggtgggc cggtaatact gtgtaggagt ccccaagctc gataactcta cccagagagt
     1801 tcaggtaggc tcctaaaccg tataagcctg agggatcttt cagaaattcc gggagatttt
     1861 tagatatagc atctctgaaa accattttac tcgtcgggtg aggggttatt tcaggtaata
     1921 tatggtcacc agtaaatatt aaatcatcca agactaggca aatttggtct ggagagtgac
     1981 cgggtgtact cattacttta aggtctgtga atacgtcccc gtccttcaaa tcttttgata
     2041 attgggtctg tctgcgatct tcaaagtatt ttttatcagt gtctctccga tcattatcat
     2101 tttcattagt tatctgaatt ttttcttcaa caatccgctc taattccctt tgcagaattg
     2161 aacctttccg gtaatgaatc tcccatggga tgtattcttt tagtggtcca tatgtctgat
     2221 gagcccacaa ttctgcagac gag
//
LOCUS       DASCNS010000222         2365 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001739013, whole genome shotgun sequence.
ACCESSION   DASCNS010000222 DASCNS010000000
VERSION     DASCNS010000222.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001739013"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="QF838_08095"
     CDS             complement(<1..561)
                     /locus_tag="QF838_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO60676.1"
                     /translation="MKPHYTNSQFTIHKITCGPYDNNAYIVICNRTNESLLIDTPAEP
                     SVLVAVASTTQVKYIAITHNHMDHLLGFEEVTKAFQAKVLIGESDSNELPVAPDLFLE
                     DGMNIQIGDLSVRAIHTPGHTEGSTCLLLNGHLFTGDTLFPGGPGKTKAPDKLKEIIE
                     SITGRLFILGDDVAFYPGHGNDGLMGT"
     gene            688..936
                     /locus_tag="QF838_08100"
     CDS             688..936
                     /locus_tag="QF838_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60677.1"
                     /translation="MSSYGITIKNASEGNRITLTCEHNGGIIYIVPSESSWVCREENI
                     GAHAITGFFEDLTNIESTQVTALMQKWGLYYRTLDVIA"
     gene            complement(951..1985)
                     /locus_tag="QF838_08105"
     CDS             complement(951..1985)
                     /locus_tag="QF838_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJO60678.1"
                     /translation="MNAKELSSILSLRKERSKEADQLMHYGLFQEAVDVNLSMIDINH
                     GDQKAYNRLGDALLKIGKSAEAYDAYQSSIFLEKTRKENTKFAVDSAMRSDWNKAIQV
                     NSDIIDRFPWDLEPYNRLGKAWSEKGQNKKAIQAFQCALVISPNSPIAKRNINRLQRT
                     SGLKANMAISATTPERSFIEETGRTGVTKLVNIPRNFDVTNLISGHSVDLISVDRGMR
                     ILDRKGMEIGSIEPKLALRLKKLVEGGNTYSANITSAAEDGVTVIIRETYRHPSQSNK
                     SSFPAKSSVLGDIPMSALGYGLNDVGKLADLKDWSDDDTESGDDEVFSPMIPKILSGD
                     SSLDNSGILD"
     gene            complement(2092..>2365)
                     /locus_tag="QF838_08110"
     CDS             complement(2092..>2365)
                     /locus_tag="QF838_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HJO60679.1"
                     /translation="CTSGFKVFKSQVLKDICWDEIHCSGFGFQIEIAMQCESLGFTIQ
                     EYPIIFNDRVSGDSKMSIGIVLEAIFKIIFGRLKIFKIIKFMKPGT"
BASE COUNT          691 a          497 c          423 g          754 t
ORIGIN      
        1 tgtgcccatc aagccatcat tcccatgccc tggatagaaa gcaacatcgt ctcccaaaat
       61 aaatagcctg ccagttatgc tctcaattat ctctttcaat ttatcaggtg ctttagtttt
      121 acccggaccg ccaggaaaaa gagtgtctcc agtaaacagg tgcccgttca gaagtaaaca
      181 tgtagaccct tcagtatggc caggggtatg aattgccctg accgataaat ctcctatttg
      241 tatattcata ccatcctcca aaaataaatc aggggctact ggaagttcat tagaatcaga
      301 ttccccaatt agaaccttag cttgaaaagc ttttgtgacc tcttcaaatc ccagcaaatg
      361 atccatgtga ttatgagtaa tggcaatata cttaacttgg gtggtagatg ctacggctac
      421 caagacagaa ggttcagctg gtgtgtctat caaaagactt tcattagttc gattacatat
      481 gactatatag gcattgttat catatggtcc acacgtaatt ttatgaatag tgaactggga
      541 gttggtgtaa tgtggtttca tcattccatc ctaatttttt tatattaaaa tttacgttgg
      601 tttagtagac ataggaatta tatcagtcag tttttattac ttaaatgggc ctatgaaccc
      661 gcataaacaa agtcgtaagg aagaaaaatg agtagctatg gaatcacaat aaaaaatgcc
      721 tccgaaggaa accgtattac tctcacatgc gagcacaacg gtggaataat atacatagtt
      781 cctagtgaat caagttgggt atgcagggag gaaaacatag gtgctcatgc aatcactggt
      841 ttttttgagg atcttactaa tatagaaagc acacaggtaa ccgcactaat gcaaaagtgg
      901 gggctttatt atcggacact ggatgttatt gcataaaggc gagtgaaaat tcagtccaat
      961 attcccgaat tatctaagga tgaatcgcca gacaaaattt ttgggatcat aggtgaaaac
     1021 acctcgtcat ctccagattc tgtatcatca tcagaccaat ctttgaggtc tgctagtttt
     1081 cctacgtcgt tgagtccata accgagagct gacataggaa tatcccccaa aacgctggat
     1141 ttggcgggaa acgaggactt attagattga gatggatgcc tatatgtttc tctgataatg
     1201 acagtaacac cgtcttctgc tgcacttgtt atgtttgcgg aataagtatt acctccctcc
     1261 accaattttt tcagtcttag ggcaagcttt ggttctatgc tacctatttc catccctttt
     1321 ctatctaata tcctcatacc tctatctact gatatcaaat ctacactatg cccagaaatc
     1381 aaattagtca catcgaaatt tcgggggata tttactaatt tcgttacacc ggtccgacct
     1441 gtttcttcaa taaaggatct ttccggtgtt gtagctgaaa tcgccatatt tgccttaagt
     1501 ccagaagttc tttgtagccg attgatattt ctttttgcta tcggactgtt aggagaaatc
     1561 acaagagcac actggaaggc ttggatagct tttttatttt gccctttttc actccaagct
     1621 tttccaagtc gattatatgg ctccaagtcc cacggaaacc tgtcaatgat gtcagaattg
     1681 acttgaatgg ctttgttcca atcagatcgc atggcactat ctaccgcgaa ttttgtgttt
     1741 tctttccttg tcttctctag gaatatggag gattggtaag catcataggc ttctgcactt
     1801 ttccctatct ttagcaaggc atcccctagt ctgttatatg ccttttggtc gccatgattg
     1861 atatcgatca tggataaatt cacatctaca gcctcttgaa aaagcccgta atgcatcaat
     1921 tgatcggctt ctttagatct ctcttttcta agacttagaa tggaagataa ttcttttgcg
     1981 ttcacttcac tcaccctttc aactcatctt ttgatttcat gagggcattc taatcagggt
     2041 tttttcttca gtgcaatata ttttgcaccc ataatcacag caaacttcca gctatgtacc
     2101 gggtttcatg aacttgataa ttttaaaaat tttgagtctt ccaaaaataa tcttaaaaat
     2161 tgcttctaat actattccaa tggacatctt tgaatctcca gatacccgat cgttaaaaat
     2221 tattggatac tcttgaattg taaaacctag actttcacac tgcatggcaa tttcaatttg
     2281 aaatccaaac cccgaacaat gaatttcatc ccaacaaata tctttcagca cctgagactt
     2341 aaaaacctta aacccagatg tacag
//
LOCUS       DASCNS010000223         1228 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001740293, whole genome shotgun sequence.
ACCESSION   DASCNS010000223 DASCNS010000000
VERSION     DASCNS010000223.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001740293"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            39..>1228
                     /locus_tag="QF838_08115"
     CDS             39..>1228
                     /locus_tag="QF838_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HJO60680.1"
                     /translation="MTSQDLPETDPDTIELLEALPSTVRELVDAQYAALAIMNEEGRI
                     IHFFFAGISEEEAGSVGDLPHGHGLLGFLNNNGKTIRVSHMGAHPSSTGFPKNHPVMI
                     SFLGVPIIRAGRNYGNLYLTNKQGAEEFTHRDQKIVEVMAEYIALAIENDRLFRAEYR
                     LREQANFQTQSLNTMIDNVPVGILVASMPGAAIIFSNDEIKNIFNSSFEIGDKIDFSD
                     DDITWMHEDGTSLPINTDPLSASISAKQELRDQIMVIDRENPSQTILSVDAVPIQQID
                     QTVLLIVINDITHIHEVERLKNEFLSMVTHDLRGPLATIRSLTQTLNHQVSPSEQYTT
                     TQALSEEAENMSDLVDNLLNMARIESGAIPFDPESCHIIDIAEECKRQFERSRYGTSH
                     RLHL"
BASE COUNT          389 a          260 c          251 g          328 t
ORIGIN      
        1 tgttagacac aggaaattct aggtgttaag gttctaccat gacctcccaa gaccttcctg
       61 aaactgaccc ggatacaatc gagctattgg aggctttacc ctctacggtt agagagcttg
      121 ttgatgctca atatgctgct ttagcgataa tgaatgagga aggtcgaata atccatttct
      181 tttttgccgg tatttcggag gaagaagccg gtagcgttgg agaccttccc catggccacg
      241 ggttacttgg attcctaaat aacaatggca aaactattag agtttctcat atgggagctc
      301 atcctagctc cacaggcttt cccaagaatc atccagttat gatctcattt ttgggcgtac
      361 ctataatcag ggcaggtagg aattatggca acctctattt aacaaacaag cagggagcag
      421 aagagttcac ccatagagat caaaagatcg ttgaagtcat ggctgaatat attgctttgg
      481 caattgaaaa tgaccgtcta tttagagcag aatatcggct aagagagcaa gctaatttcc
      541 aaactcaaag ccttaacaca atgatcgata atgttccggt gggaatttta gtggcttcta
      601 tgccaggagc cgcgattatt ttttcaaatg atgaaatcaa aaatatattc aattcctcat
      661 ttgaaatcgg agataaaatc gatttttccg atgacgatat aacttggatg catgaagatg
      721 gaaccagcct tcccattaat acagatcccc tatctgcctc tatatccgcc aaacaagagc
      781 taagggatca aataatggtg attgatagag aaaacccgtc tcagactatc ctatctgttg
      841 acgctgtacc tatccaacaa atagatcaga cagtccttct tattgttata aacgacatta
      901 ctcacataca tgaagtagaa agactgaaga acgaattttt atcaatggtg acccatgacc
      961 tccgtgggcc cttagcgaca atacgaagcc taacgcaaac cttgaaccat caagtctcac
     1021 cttcagagca atacaccaca actcaagcac tatctgaaga agcagaaaat atgtctgatt
     1081 tggtcgataa tctactgaat atggctagga ttgagagtgg agcaatccca tttgaccccg
     1141 aatcgtgcca cataatcgat atagcagagg aatgcaaaag acagtttgag cgtagtcgat
     1201 acggtacgtc gcatagactt catttaga
//
LOCUS       DASCNS010000224         3032 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001747541, whole genome shotgun sequence.
ACCESSION   DASCNS010000224 DASCNS010000000
VERSION     DASCNS010000224.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001747541"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="QF838_08120"
     CDS             complement(<1..490)
                     /locus_tag="QF838_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJO60681.1"
                     /translation="MKFHTSALIKPSSSRCNLDCTYCFYLKKQEMYPWSDNPSLTLET
                     FEIFLKGYIPLSAPHLTFLWQGGEPTIMGLQFFDAAMELERKVARGMLPTAIPHISNA
                     IQTNGTLLTQEWAKFFKKWRFLVGVSLDGPQEWHDRYRIDPIQRKTHSNVMEGIKHLE
                     DFK"
     gene            complement(744..1796)
                     /locus_tag="QF838_08125"
     CDS             complement(744..1796)
                     /locus_tag="QF838_08125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60682.1"
                     /translation="MKFGLFYLFSDFGNIPQSQVYEEVLDEIEFAESIGFDSVWLPEH
                     HFSTYAMMGNPLTFAAAVSQRTTKMRIGTGVALLPFQHPLRIAEDAALVDSISGGRLM
                     LGVGRGYQPPEFQGFGIPQEDSSGMFQESIEILSRALKGTKFTYSGKYWSIEEPTEIY
                     PKPIQSPHPPFYIASVTPRSLSVAAQYGMSLIRGPQFTNLETVAAGYETYRELMGNNG
                     YDPDVLDQPLSIRTYVAPTEDEAWEEADNAVWFYRLMATLLPGAPGRPKPMSGYENYP
                     QDPAILAKTTVSDLKERGTAFGSPESVAEILNTYISRLNPSHLMLQMRIGGLEHEKVK
                     RSMRLFINEVVPRLIV"
     gene            complement(1899..2807)
                     /locus_tag="QF838_08130"
     CDS             complement(1899..2807)
                     /locus_tag="QF838_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HJO60683.1"
                     /translation="MKLNRILVPLDDSANSEEVLSFVTQIACANPKAEVTLITITNLD
                     SAGDSASVFKLNAFTDLLASKGIESHFIITESLSTADKINEWADNYGYDLIAMSTKGR
                     GGLTRAILGSITTEVIRLATIPVMVVAAGRSNKHRLNPPKLNEIVVPLDGSLLSESSL
                     PFAENLAKTLDIGIRVMNVVTPVELTVPSPIDESPYFVHNKIMPEVYENAKEYLADIS
                     NTLTGKGLNVLSRNVLTGNTASSLIGSIKDPVESLFVISTHGKSGVDRLLMGSVTDSI
                     LRSYGGPVFVIPANSSRALTQAPVGS"
BASE COUNT          844 a          655 c          616 g          917 t
ORIGIN      
        1 ctttaaagtc ctctagatgt tttatacctt ccattacgtt cgaatgagtt tttctctgga
       61 ttgggtctat cctgtagcga tcatgccatt cctgagggcc atcaaggctt actcccacaa
      121 ggaatctcca ttttttaaag aatttagccc actcttgcgt taaaagagtg ccattggttt
      181 gtatagcgtt actaatatgg ggtatggccg tagggagcat acccctcgcc actttccttt
      241 ctagttccat tgcagcgtca aaaaattgga gacccattat tgtaggttcc ccaccttgcc
      301 aaagaaaggt taaatgagga gcggaaagag ggatgtatcc cttcaaaaaa atttcgaatg
      361 tttcaagtgt taaagaggga ttatcagacc aagggtacat ttcctgtttc ttgagataaa
      421 agcaatatgt gcaatccaaa ttacacctac tggaagacgg ttttattaag gcactcgtat
      481 gaaacttcat ggcaaacttg atttaggctt atttggaaca ggcattaaag tcatgaattc
      541 ttgagtattc gcaaaattat gattcctatt cttggagttt ttcatctttc gagacccatt
      601 tatagggtca ctatcgaacc cgcattggat acaagataaa taatcccagt atggcccctc
      661 ttggtttgac atgtccccac cgcacttggc acaggatttg aaatatatca tttggttccc
      721 ctctgatatt ctaagaaact aattcagacg attaacctag gaaccacctc atttatgaaa
      781 agtcgcattg atctcttcac cttttcgtgc tccaatcctc caattctcat ttgaagcatt
      841 aaatgggaag ggtttagtct actgatatag gtattgagaa tttcagcaac actttccggt
      901 gaaccaaaag cggtacctct ttcttttaaa tcagaaacgg tcgtttttgc cagaattgcc
      961 gggtcctggg ggtagttttc atatccagac ataggctttg gtcttcctgg agctcccggg
     1021 aggagtgtcg ccatcaacct gtaaaaccaa accgcattat cggcttcttc ccacgcctca
     1081 tcttcggtcg gagcaacgta agtccgtatg gatagaggtt gatcaagtac gtctgggtcg
     1141 taaccattgt tccccatcag ttcgcggtat gtttcatatc cagctgccac agtttctaaa
     1201 ttggtaaatt ggggacccct aatgagagac atgccgtatt gtgctgccac actcagactt
     1261 ctgggggtaa cactggctat ataaaaggga ggatgtggcg attggatagg tttcggatat
     1321 atttcagttg gttcttcgat agaccaatat tttccactat atgtaaattt ggtacctttc
     1381 aaggctcgag acaatatttc aatcgactcc tgaaacatgc cggaagaatc ttcttgaggg
     1441 attccaaacc cttgaaattc tggtggttga taccctcttc ccacacccaa cattaatctt
     1501 cctccactta tcgagtccac aagagcagcg tcctccgcaa tcctcaaagg gtgttggaat
     1561 ggcagtaaag cgactccggt gccaattctc atctttgttg ttctttgaga aaccgcggca
     1621 gcaaaggtta atggattccc catcatagcg tatgttgaga aatggtgttc tggtaaccat
     1681 acagaatcaa atccaattga ttctgcaaat tcaatttcat ctaacacttc ttcatatact
     1741 tgtgattgag gaatattccc gaaatcagaa aaaagataaa aaagaccaaa tttcactgtt
     1801 tgactccttc agaaagataa cgaaatggtt gtttggttaa tgagttgata ctagtggtca
     1861 taatagaatt tcttcacata gcccgaggtt tgcaatattc aagatcctac tggtgcttga
     1921 gttaaggctc tgcttgaatt tgcaggaatt acgaaaacgg ggcctccgta agagcggagt
     1981 atagaatctg tgacacttcc cattaataat ctatcaacgc cactcttgcc atgggtactg
     2041 atgacgaata gggactcaac cggatcctta atacttccga taagtgaact agctgtgttg
     2101 ccggtcagta catttcggct taggacattg aggccttttc ctgtgagagt gtttgaaatg
     2161 tcagctaggt actctttagc attttcatag acctctggca ttattttgtt atgaacaaaa
     2221 tatggagatt catcgattgg gcttggtacc gttaactcaa ctggagtcac tacattcatc
     2281 acacggatcc ctatatctaa agttttagct aaattctcgg caaatggaag gctagattct
     2341 gacagaagag acccatctag cgggacaaca atctcattta atttaggtgg attcagcctg
     2401 tgcttattac tcctcccggc tgctacgacc ataactggga tggtggccaa cctgataact
     2461 tctgtagtta tactgcccaa tatagctcgg gtcaatcccc ccctaccctt ggttgacatg
     2521 gcgatcaagt cgtaaccata gttgtcagcc cattcattta tcttatctgc cgtactcaat
     2581 gattcagtga taataaaatg gctttctata cctttggagg ccagtagatc tgtaaatgca
     2641 tttaatttga agactgaggc agaatctcca gcggaatcca agtttgtaat tgttattaaa
     2701 gtaacctcag ctttagggtt agcacaggcg atttgagtta caaaagaaag cacttcctct
     2761 gaatttgcag aatcatccag tggcactagg attctattta atttcatgaa acctccccat
     2821 tccatacatt cctattcttt atgaaagcca cattaaccga aaagccgtga accagtgatg
     2881 aagaatagct acgctattgt gaaaaattcg tgttcatgga cgatccaatt caatcctgta
     2941 gacttcttaa aaaggctatg attgcttcaa tatcagaatc ggaaagagct ggattacctc
     3001 ctttgggagg catgtccact ccagtgctat tg
//
LOCUS       DASCNS010000225        12984 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001758436, whole genome shotgun sequence.
ACCESSION   DASCNS010000225 DASCNS010000000
VERSION     DASCNS010000225.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12984)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001758436"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="QF838_08135"
     CDS             <1..849
                     /locus_tag="QF838_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016119.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HJO60684.1"
                     /translation="LRTPAAELESLNAEYGNPTINAVHGITPTPNMNQLDQTMKRKTI
                     SETGTIDINEALIVHEDIKLNPQPDQYEVENFSELLDGYGENAPLRKIHPKSWSDPNW
                     LLPDKFDTSDYTQGSFAFSVIDISDISVELLSGIPGKISIPILNLTAPVENLAIIEKD
                     GRMAYETPKNLVGRIPSSPNQSDTFTGWYFGHLESPLKGEGNVFHNLPAIAEHLLNGN
                     EVLISLEKPEKKLVYQVTKSEVIHESDLKLYDPGLGKIILVTCSNRPIYDHRQLVTAT
                     LVGVVE"
     gene            complement(850..1731)
                     /locus_tag="QF838_08140"
     CDS             complement(850..1731)
                     /locus_tag="QF838_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HJO60685.1"
                     /translation="MELRELKSFVAAAKLRSISKAAKELNIGQPTVTTHIKKLERELS
                     VPLFDRVTRPIRLTLTGQTIYELSNPLLEGIDSLSSIISLAEERGPVTIASTPDIIPH
                     TLLSVVQKFNKLYPAVYLRIQSANRNEVTNLVRTGEVDIGIIQHPERGDDLHFEPLFL
                     YERVLIAPKGHELLSRNIQSLEPIARYPLILMAKGTYTRTILEEQLQKDALKYEVIME
                     LERMDMIKKYVALGMGVSIGPRLAIEDDDLSKLGMVSLANFLPVDQAGILTLPGKNMS
                     DPAEKFMEVMRTMLVTK"
     gene            1906..2829
                     /gene="ftsY"
                     /locus_tag="QF838_08145"
     CDS             1906..2829
                     /gene="ftsY"
                     /locus_tag="QF838_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HJO60686.1"
                     /translation="MNVFKKRNPTKFTESLEKTRSSLVGQLGDLFKKSELDTEFWEDL
                     EGTLISADTGLETAEKIVSQLRENAKAKNISTGKECLKELRSELKNIFDLKDEDPLTR
                     AQKPVVFVMVGVNGAGKTTSIAKLTHLLISLNQKVILGAADTFRAAAVDQIQIWGDKL
                     GVTVIAQDPGSDPGAVAFDTIKAAKSRNMDVAIIDTAGRLQGKSNLMEELKKISKIII
                     RESDESSVKIILTIDGTTGQNGLIQAIEFTKFVSPDGIFLSKLDGTAKGGIAIAIADQ
                     LKIPIWFVGTGEQPDDIAVFDPNLFISRIID"
     gene            2855..5146
                     /locus_tag="QF838_08150"
     CDS             2855..5146
                     /locus_tag="QF838_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HJO60687.1"
                     /translation="MLYGITWLILIEVVGFATFVIFLSIFRTIPDKGYSISKPLGIIC
                     LSLVTWLLSISKIIPATDITTILVFIGLIICSLTIGTIRVKSLKQIVKNNWRIISLTE
                     LIFLLTYLIFFSIRYFDPGINHTEQPMDFAFLNSSIRTVSGQPLDPWFHGDTISYYYF
                     GYWIFGTLSKISLIPTIATYNLSLVAIPAMTASSIFTLTSILLRFSRVKFDFLKISCG
                     VIASLTAVFMGNLQGFLEFLRINSIGSSGFWQRVCIDGMQNPAINTIDSWRPTEFWWW
                     FRSSRIINYFGPACEGQSFDYTINEFPFFSYLLGDLHPHVMATPFVLTFVYIAYDLSK
                     KDLTKPLGIMYWLEVALAGVMLGCVSFINMWIAPVCIAIILGIYSLHWLSNSNLNPIK
                     LAKSISLVFFLGALVLLPYLWSFQSSIDSLSKTQYQTSIIHGFVVWSPLLILSIPLVV
                     GTFWTTHISTAWKYHLGISFTVVSIPWMIRFLIPEANIDSEGPGMLGFAFPITILCFI
                     STLTLLTLTTRKGVSRRIIVLSIFSLSMFLILIPELFYVGDVYGNRMNTVFKLYYPAW
                     ILLSICGSYSVYYWLAGYIRPQKFLKYLYTSIAAIIILCAFFYTPAATMTKINESSIS
                     GFKNSTARLTDLEVSALAYANQNISLDQVILESVGEWDGSGFISRNTGIPNLVNWPGH
                     ESQWRDSDPAIYQRAADVETIYSTRDLAQAKSLLGKYEINFIYVGDLELNRYTAEQLE
                     KFQSLGTIVFGDLGSVSIFEIDR"
     gene            5143..7323
                     /locus_tag="QF838_08155"
     CDS             5143..7323
                     /locus_tag="QF838_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HJO60688.1"
                     /translation="MKYIQRYFHIKKMANITRIEGLLYLGLFALALSLRLYDLSAKAM
                     HHDESLHAYYSWELFQGSGLIHNPMLHGPLQMQLTSLIFFLFGDTDVTARILYVSAGT
                     ILVILPILFRNLLGKHGAIMVAVLLSISPSMVYFSRFARNDILMALFTFGMVITMWNY
                     LISGNKKNLYLMSGLLALSFSTKENAYLIVGTLGLYLTMASIHESWPKKTYGYQLQSI
                     SYPRLIFVYAMMILKAGKNCIYIYPRSRTFTVLILLISLTLPQWSAFAGIFQETILLR
                     WSNIILVSGEGASNIGMPTHGGKLLAFLIVFSLIASSIYIGYKWQWKTWWKCATIFYL
                     IWVFSYTTFFTNIFNGVQSGIWQSLGYWIVQQGEARGGQPTYYYLTLIPIYEYLPAVF
                     SVLASLYFLKHRSKFNLFLIYWTAVTLFLYTVASEKMPWLLVNISLPMIVLSGKFLGD
                     LFSKWNIKSKPNLNHCMIYIIPTIALIVFFGVTKYSSNLHPIPRMFTAIIMLSLILSS
                     FVFMVRFIRRHETHTLTKYFYAGFATVLLILTIRTTIYSSFINPDIPIEMLVYTQTSP
                     DLLKVNNKMKTLYAKSEMHNEPLIIIDQTNGFTWPWSWYLRNYSNVKYPKMHAESYEP
                     HEPATMVVVHSSNHLAADKALSKNYKKAGRIPHRWWFPEHTYRNLNAISLASKLTDIK
                     HWNTFLEYWLFRKGVGKSIGSEDAYIYTSNAFPNLDFVGDAYRE"
     gene            7364..8392
                     /locus_tag="QF838_08160"
     CDS             7364..8392
                     /locus_tag="QF838_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HJO60689.1"
                     /translation="MKKSSLWVFTGVLLLIIFALTIDVGEMWNTLKKANYIVIVPGLL
                     AYFLGVWFRTLRWQVLLTGIDEIKIRTLFPIVVIGYMANNILPFRLGELIRGYYLKNK
                     EGVPTATALSTIFVERIMDGLALILILAASYTSVPFDEIFSRLSQITRLDETFLLVIF
                     TFPFAILFGLLVAIALNGPQSMRIFGHLTSRLPRNLARAINKLTEDVVAGLYSLRNWK
                     ITCKALLLSLPIWILEAVLFHSVLVSLGILPTDSGVWHIFASAASITAITNIGASIPS
                     MPGGIGLFEMIARETLMVIEPTSISRSEAAAFAALTHLCLIVPIVLLGQLFLWVEGLS
                     LRKLRHRI"
     gene            8427..9746
                     /locus_tag="QF838_08165"
     CDS             8427..9746
                     /locus_tag="QF838_08165"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJO60690.1"
                     /translation="MKFGIIGAGATGLAAAYDLSSKGHEVKIYEASDFIGGHASTFQV
                     AGSELEKGYHHWFTNDTDIISLIGEVGLKEIIEWFPSSVGTYQDGKIYKFLSPFDLMK
                     YKPLGFIDRIRMGLSSLQIRYIKDWHKLESISAKEWLIRNTNREIYHAFWEPMLRGKF
                     GEENYEKVSMAWIWGKMTTRFASRKNILAKEVLGYPNGSFSHLFQLLAKTCESNSAEI
                     FLSTQIKRIRGSNSGEVFMTIGQQGEEEAFDAVIASTPSHIFNCLTEGLSKEYRSKLL
                     NIKYMAAVLIVLVLKHPLSDVYWLNIADRSVPFIGIIEHTNLVGSERYKGRNIVYLSN
                     YLSEDHELYKMTGSELFDTYLPHLKKINPDFTADWVKEYHHHKIPNAQPVVDTNYGKN
                     IPAHETGIQNLYLANTSQVYPQDRGTNYSVAMGRKMAHLALLNLKNE"
     gene            9812..10303
                     /locus_tag="QF838_08170"
     CDS             9812..10303
                     /locus_tag="QF838_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HJO60691.1"
                     /translation="MTTDKDNLRTKIQAAIQSQKRPTVTVLSSLLAIQDSIGYIPEEA
                     IEEVAVNSNTSINSVFGVASFYPNFRFTPPGEHSVEVCWGPTCHLKGATEVLESVLEK
                     LGLDSEGDTDDGKVSFRFNTCLGACSQAPVISVDHELYGNAKPDIIPNLLGSLHEKEN
                     HHQ"
     gene            10318..12054
                     /locus_tag="QF838_08175"
     CDS             10318..12054
                     /locus_tag="QF838_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013662.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HJO60692.1"
                     /translation="MKYEEITSQATAEWADMTSGRVPLIRIGTAMCGHAAGAFRVVKA
                     LRKCLESKGIEANIQEVGCLGLCYAEPLLDIKKEGKSRLFFNNVTPEEVEYIVDEYLL
                     KEGYPKEKVFGYIGEEGPVSGEDSLEMLPGLKLQHRIALRNAGHTSPYDINQYIANGG
                     YSGLYKALTEMSPSEVIDEVKNSGLRGRGGAAFPTGVKWSFLVGSPGPTKYILCNCEE
                     GDPGAYNDKGILESDPHTLLEGLAIAGYATGASNGIVFIRHGHDGPIERTEKAIEQAY
                     DLGLIGNNILGTDFSFDIEVALTGESYVAGEETALMEAVEGKRAMPRFRPPFPAAFGV
                     WGKPSNINNVKSLSYAPEIISKGADWFSSIGVNQSTGTAIVCLSGDVNRPGLYEVPMG
                     LTLGEVIDDLGGGVTDNAQLKLLQTGGPLGGVLGPDSKDIHIDFDEMRSAGAIFGSGG
                     IIVGSDKVCAVDLTRILVAFCQFESCGKCFPCRLGMEHLLEIVERISNCDSKPGDMDL
                     MKSIGGTMEAGSLCGHGQLGFGPIRSAITHFEEDFRLHIEENRCPTGSCDRLKIVPKN
                     TRPYATDHIAGE"
     gene            12056..>12984
                     /locus_tag="QF838_08180"
     CDS             12056..>12984
                     /locus_tag="QF838_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024900.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HJO60693.1"
                     /translation="MSDKVNLTIDGVEIQAEPGSMIIQAAMDNEMYIPYLCYYPGMKP
                     YGACRMCVVKAEAPTPDGSYRALPGSPASCTTPVSEGMRVETNTGDLVGLRKGIMELL
                     ISEHPHGCLNCHRIELCGPTDVCLRHVSVNDRCVTCPKNERCELKDTVRYMEMELTTP
                     LTYNNRHLPLEVKDPYWDMDMNLCIVCSRCVRACDEVRGDRAITLMDKSGRSIIGTAA
                     GTSLLESGCEFCGACIDVCPTGAIVERDYKWDKAVQKVSTTCPHCPVGCQLDLDVNKR
                     NKVVRSIPNRQAEANMGMDCFKGKFGLDFVNNK"
BASE COUNT         3936 a         2609 c         2681 g         3758 t
ORIGIN      
        1 ctaagaacac ccgccgccga acttgaatcc ttaaacgcgg aatatggaaa cccgactatc
       61 aatgctgttc atggaatcac tcccactcca aacatgaacc aattagacca aaccatgaaa
      121 agaaaaacta tctctgaaac aggaacaata gatattaatg aagctttaat tgtccatgag
      181 gatattaagc tcaaccctca acccgaccag tatgaagtgg aaaatttttc tgagctgcta
      241 gatggatacg gggaaaatgc gcccctgagg aaaatacacc ccaaaagctg gtcagatcct
      301 aattggcttt tgcctgacaa atttgatacc tcagactata cccaaggtag tttcgctttc
      361 agtgtgatcg atatttctga tatcagtgtt gaattgcttt caggcatacc tgggaaaatc
      421 tcaataccga tactgaacct gacagcccct gtggaaaatt tagcgatcat agaaaaagat
      481 ggccgcatgg cctacgaaac cccaaaaaat ttagtaggtc gcataccatc atctcccaat
      541 caatctgaca ccttcacagg atggtatttc gggcacctag aaagtcctct aaaaggagag
      601 ggaaacgttt ttcataactt acccgcaatt gctgaacatt tgctcaacgg taatgaagta
      661 ctaatttctt tagaaaaacc tgaaaaaaag ctagtgtatc aagtaacaaa atccgaagta
      721 attcatgaat cagacctaaa attgtacgat cccggtctgg gaaagataat tttagtgacc
      781 tgctccaaca ggccgatata cgaccacaga caattggtca cagctactct tgtcggagta
      841 gtggaatagt tatttcgtaa ccaacattgt tctcataact tccatgaact tttcagccgg
      901 atctgacata ttcttccctg gtagagtcag tattccagcc tgatctaccg gcaagaaatt
      961 agcgagggaa accatcccaa gcttagataa atcatcgtct tcaatggcca atcttggccc
     1021 tattgacact cccatgccaa gagcaacata tttcttaatc atgtccattc tctctaactc
     1081 catgattacc tcatatttga gagcgtcttt ctgaagctgt tcttctagaa tggtcctagt
     1141 ataagtaccc ttagccatca aaattaaagg atacctagca attggctcca atgactgaat
     1201 atttcttgag agtaattcgt gtccttttgg tgctataagc accctctcat aaagaaataa
     1261 aggttcaaaa tgtaaatcat cccctctttc tggatgctgt attatgccaa tgtctacttc
     1321 ccctgtccgt accaaattgg ttacttcatt tctattggcg gattgaattc ttaaatacac
     1381 cgctgggtat agcttattaa atttctgtac gacactaagt aaagtgtgcg gaataatatc
     1441 tggagtcgat gcaatggtca ccggccctct ttcctcagcc aatgaaatta tcgacgacaa
     1501 agaatctatt ccttctaaaa ggggattgga aagctcatat atcgtttggc cagttaaggt
     1561 aaggcgaata ggtcttgtca ccctgtcgaa caggggaact gacaattctc tctccaattt
     1621 ttttatgtgg gtcgtgacag taggttgtcc aatattcaat tcctttgccg ctttagaaat
     1681 acttctaagt ttggcagctg cgacaaaact ttttaattct ctcagttcca tgaggttaat
     1741 ttctcgcttt attacaaatc ttgatgcatg atagtgtaca ttatttcatg gcgccatata
     1801 aataacagac ttaaattggc ttgcgatata gaataaaatt tgtaatcgct tttgatattt
     1861 aaatccattc accctgaccg ctttaaaatt tgataaatat tacaaatgaa cgttttcaaa
     1921 aaaagaaacc ccaccaaatt cactgaatct cttgaaaaaa cccgaagtag tttagtcggc
     1981 caattaggag atctattcaa aaaatcagaa ctagacactg aattctggga ggatctagag
     2041 ggcacattga tttcagcaga taccgggtta gaaaccgctg aaaagatagt ttcccaactc
     2101 cgagaaaacg caaaagcaaa aaacatatcc accggtaaag aatgtctgaa agaacttcga
     2161 agtgaattga aaaatatatt cgacctcaaa gacgaagatc cacttactag agctcaaaag
     2221 ccagttgttt tcgtgatggt gggtgtgaat ggggcaggta aaactacatc gatagcaaaa
     2281 ttgactcatt tactaataag tctgaatcaa aaggtaatat taggtgctgc tgataccttt
     2341 cgtgcagcag ctgtggacca aatacagatt tggggagata aattgggagt tactgttatt
     2401 gctcaagatc cagggtcaga tccaggggcc gtggcttttg atactatcaa ggccgcaaaa
     2461 agtagaaata tggatgttgc catcatcgac acagcaggta gactgcaagg caaatcaaat
     2521 ctaatggaag agctcaaaaa gatatcaaaa ataatcatca gagaatccga tgaatcctca
     2581 gtgaaaatta tcctcacaat agacggcact acggggcaga acggtctaat ccaagctatt
     2641 gaattcacaa aatttgtgag tccagacgga atatttctca gcaaattaga tgggactgct
     2701 aaagggggaa tagcgatagc tattgctgat cagttaaaga tccccatatg gttcgttgga
     2761 acaggcgaac agcctgatga cattgcagtt ttcgatccca atttgtttat atccaggata
     2821 atagactaaa tagtaaaata gagacgattt cataatgctt tacggcataa catggctgat
     2881 tttaatagaa gttgtgggat ttgctacatt tgtaattttc cttagtatct ttagaacgat
     2941 acctgataag ggttattcaa tcagcaaacc cctcggcata atatgcctgt ccctcgtgac
     3001 atggttactt tcgatttcca aaataatccc ggccaccgat attacaacaa tactagtttt
     3061 tatcgggtta attatatgtt ccttaactat aggcacaatt cgagttaaga gtttaaaaca
     3121 aattgtgaaa aataattggc gaataatttc cttaacagaa ttgatattcc tgctaaccta
     3181 tctgattttt tttagcatta gatactttga ccctggaata aatcacactg aacaaccgat
     3241 ggattttgct tttttaaact catctataag aaccgtaagt ggacaacctc ttgatccttg
     3301 gttccatggt gacaccatca gttattatta tttcggttat tggatatttg gcacactctc
     3361 aaaaatttca ttaatcccca ccatagccac atataatctt tcgttggttg caatacctgc
     3421 gatgaccgcg tcttcaatat tcacactaac ttcaatactt ttacgattct cccgagtgaa
     3481 attcgatttc ttgaaaattt cgtgcggtgt gattgcatcg ctcacagctg tatttatggg
     3541 aaacttacaa gggtttttag agttcttaag aatcaattcg attggatcat ccgggttttg
     3601 gcaaagagtt tgcattgatg ggatgcaaaa tccagcaatc aatacaatag acagctggag
     3661 acctactgaa ttttggtggt ggtttagaag ttcaagaata atcaactatt ttggccctgc
     3721 atgtgaagga caaagctttg attacactat caatgaattc cctttcttta gctatctcct
     3781 tggagatctg cacccgcacg ttatggccac gccatttgta ttgactttcg tatacattgc
     3841 ttatgattta tccaaaaaag acttaaccaa accccttgga ataatgtatt ggctggaagt
     3901 tgcattggca ggagtaatgc tagggtgtgt gagttttatc aatatgtgga tagcccctgt
     3961 ttgtatagcc ataatcctcg gaatatatag cctacattgg ctttcaaata gcaatctcaa
     4021 tccgattaaa ttagcgaaat ctatttcatt ggtattcttc ctaggtgcgc tcgtgctttt
     4081 accttaccta tggtcttttc aatcatccat cgatagccta tcaaaaaccc aatatcagac
     4141 atcaattatc catggatttg tggtttggag ccctttatta atactttcaa ttcccttagt
     4201 agtgggtact ttttggacga cccatatcag tacagcttgg aaatatcacc tgggtatttc
     4261 tttcacagta gtcagcatac cctggatgat aaggtttttg atccctgaag ccaatattga
     4321 ttccgaagga ccaggcatgc ttggttttgc tttccctata accattttat gtttcatttc
     4381 cacattgact ttattgaccc taacgactag aaaaggcgtt agtaggcgaa tcatagttct
     4441 ttcaattttc tctttatcaa tgtttctgat attaatacca gaactttttt acgtcggcga
     4501 cgtatatgga aaccgaatga acacagtttt caaactctac taccctgctt ggatactact
     4561 atcaatctgt gggagttact cagtatatta ttggttggcc ggatatatac ggcctcaaaa
     4621 gtttctcaaa tatctttaca cttcaattgc agccataatc attctatgtg ccttctttta
     4681 tacaccagct gctaccatga ctaaaattaa tgaatccagc atttcaggtt ttaaaaattc
     4741 cacggcaagg ctaacagacc tagaagtgtc ggctcttgca tatgccaacc aaaacataag
     4801 ccttgaccag gtaatattag aatccgtcgg agaatgggat ggttcgggat tcatctcccg
     4861 aaataccggt atcccaaatt tagtcaactg gccgggccat gaaagtcaat ggcgagattc
     4921 tgatcctgcc atatatcaaa gagccgctga cgtagagacg atatattcta ctagggacct
     4981 cgcccaagca aaatcattat tgggaaaata cgaaattaac ttcatttacg tgggtgatct
     5041 tgaattgaat cggtataccg ctgaacaatt agagaaattc caatctcttg gcacaatagt
     5101 gtttggagat ttgggtagtg tcagcatttt tgagattgac cgttgaaata tatacagaga
     5161 tatttccata ttaaaaaaat ggcaaacata actcgaatag aggggttgct gtatttaggc
     5221 ctgtttgctc ttgctttgtc actgcggctc tacgatctga gtgccaaggc gatgcaccat
     5281 gatgaaagcc tccatgcata ttattcatgg gaactatttc aaggatcggg gttaatacat
     5341 aatcctatgc tacatggtcc attgcaaatg cagttgacat ccttaatttt tttcctgttt
     5401 ggtgacaccg acgtaactgc tcggatccta tatgtttccg ctgggactat tctagtcatc
     5461 ctacccatac tttttagaaa tctactcggc aaacatgggg caataatggt cgccgtatta
     5521 ctgtccatat ccccttccat ggtttacttc agcaggtttg ctcgaaatga catacttatg
     5581 gcgcttttta cttttggcat ggtcattact atgtggaatt accttatttc cggcaacaaa
     5641 aaaaatcttt atttgatgtc tgggcttcta gctttaagtt tttcaaccaa ggaaaatgct
     5701 tatttgattg tcggtacttt gggattgtat ttaacaatgg cttccatcca cgaatcatgg
     5761 ccaaaaaaaa cttacggata ccaacttcaa agcatatcct atccccgctt aattttcgtt
     5821 tatgccatga tgatacttaa ggccggcaag aattgcatat atatctatcc gcgcagccga
     5881 acattcacag tactaatact tctgatttca ttaacactac cccagtggtc agccttcgct
     5941 ggtatttttc aggaaactat attactgaga tggtccaata tcattctagt ttccggggaa
     6001 ggtgcctcca atatcggaat gccgacacac gggggtaaat tactagcctt tctaatagtt
     6061 ttctctttaa tagcctcatc tatatacatc gggtacaaat ggcaatggaa gacctggtgg
     6121 aaatgtgcca ctatatttta tttaatatgg gtgttcagtt acaccacttt cttcaccaac
     6181 attttcaacg gtgtacagtc tggaatatgg cagtcattag gctattggat agtacaacaa
     6241 ggggaagctc ggggaggcca gccgacctat tattatctaa ctctcatccc aatatacgaa
     6301 tacctaccag cagttttttc agtactggca tctctatatt ttctgaagca tcgcagtaaa
     6361 tttaatctat tcctgattta ttggaccgca gtaactctat tcctctacac ggtagcaagc
     6421 gaaaaaatgc catggctact cgtaaatatt tctttaccca tgattgtgct ttctggaaaa
     6481 tttttaggag acttattcag caaatggaac attaagtcaa aacctaattt gaaccattgt
     6541 atgatttata tcattccaac aatagcgcta atagtgtttt tcggtgttac gaaatattct
     6601 tccaacctac atccaatacc aaggatgttt actgcaataa ttatgctttc attaatcctt
     6661 tcaagctttg tgtttatggt gaggtttatt cgacgtcacg agacgcacac cttaacaaaa
     6721 tatttctatg ctggctttgc cacggtactt cttatactga ccatccgcac aactatttac
     6781 tcaagtttta taaatcctga tattcccatt gaaatgctgg tatacactca aacttcccca
     6841 gatttactca aagtcaacaa caaaatgaag acactgtatg caaagtctga aatgcacaac
     6901 gagccattaa tcatcataga tcaaacgaac ggtttcacat ggccttggtc ttggtatcta
     6961 agaaattatt ccaacgtcaa atatcctaaa atgcacgcag aatcatatga acctcacgaa
     7021 ccggctacaa tggttgtagt tcattcctct aaccatttag ctgcggacaa ggctttatct
     7081 aaaaattaca aaaaagctgg cagaatacct cataggtggt ggtttccaga acatacttac
     7141 aggaacctta acgctatcag tttagcctcc aaactcacgg atattaagca ttggaataca
     7201 tttctggaat attggttatt ccggaaagga gtaggtaaaa gcatcggaag tgaagacgca
     7261 tacatctata ctagcaatgc ttttccaaat ttagatttcg tgggagacgc ttacagagaa
     7321 taagcttcca cttaaagaga atcaccagct ataaggacag gatttgaaga agtcttcatt
     7381 gtgggtattc acaggagtat tattattaat aatattcgcc ctcaccattg atgttggtga
     7441 aatgtggaac actttaaaaa aggcaaacta tatagttata gtacccggac ttcttgctta
     7501 ttttttgggt gtgtggttcc gaacattgag atggcaagtg cttctcactg gaatagatga
     7561 aattaagatc cgaactctgt ttcctatagt tgtgattggt tatatggcga acaacattct
     7621 cccttttagg ctaggggaat tgataagagg gtattaccta aaaaacaaag aaggggttcc
     7681 gactgcaacg gcattaagca cgatctttgt agaacgtatt atggatggtc tagcccttat
     7741 acttatcctc gcagcgtcat atacgtctgt accgtttgat gagatttttt caagactatc
     7801 tcaaatcacc agactagatg aaactttctt actggtcatt tttactttcc cattcgcaat
     7861 cctatttggt ttattagtag ctattgcact taacggacct cagtctatga gaatttttgg
     7921 ccatttaacc tctcggcttc caagaaattt ggcccgtgct ataaataaac ttactgaaga
     7981 tgtagtagcg ggtctatatt ccctaagaaa ttggaaaatt acctgtaaag ccttgttact
     8041 atccttacct atttggatcc ttgaagcagt attatttcat tctgttttgg tcagtttagg
     8101 aatcctacct accgattcag gtgtatggca catttttgcc tcagcggcca gtattacagc
     8161 tataacaaac attggagcat ctataccatc gatgccaggc ggaatcggct tattcgagat
     8221 gatagcccgt gaaacactta tggtaatcga acccacaagt attagccgat cggaagccgc
     8281 agcattcgct gcccttaccc atttgtgcct aatagtgccg atagtacttt tgggccaatt
     8341 atttttatgg gtagaaggtc tttcattgag aaaacttcgt cacagaatat aaacatcacg
     8401 aagatcgaaa ctaataaagg tgttacatga aatttgggat tataggagct ggagcgacag
     8461 gcttggcagc ggcttacgat ctttcctcaa aaggtcacga ggtcaaaata tatgaagcca
     8521 gtgattttat aggagggcac gcttccacat tccaagtagc tgggagtgag cttgagaagg
     8581 gctaccatca ctggtttaca aatgacacag atataatttc tttaataggt gaagtaggtt
     8641 taaaagaaat tattgagtgg tttccgtcca gtgtcggaac atatcaagat gggaagatat
     8701 ataagttcct gagccctttt gacttgatga aatataagcc tttagggttc atagatcgga
     8761 taagaatggg attatcgtca ttacagataa ggtatataaa agactggcac aaactcgaat
     8821 ctatatcagc gaaagaatgg ctgattagga acacaaatag agagatctac catgcatttt
     8881 gggagccaat gctaagggga aaatttgggg aagaaaacta cgaaaaggtc agtatggcat
     8941 ggatatgggg aaaaatgacc actcggtttg catcaaggaa aaacattctt gctaaagagg
     9001 tcctaggata tccaaacggg agtttctctc atctttttca actactggct aaaacctgtg
     9061 aatcaaattc cgcagaaatt tttttatcga cccaaataaa aaggatccgt gggtcgaatt
     9121 ctggagaggt tttcatgact attgggcagc aaggggaaga ggaggctttt gatgctgtca
     9181 ttgcatccac gccatctcac atttttaact gcttgacgga aggtctatca aaagaatacc
     9241 gctctaaact tttaaatata aaatacatgg cggcagttct tattgttctg gtattaaagc
     9301 accctctatc tgatgtttac tggcttaaca tagctgaccg atctgttcct ttcattggca
     9361 taatcgaaca cacgaacttg gtgggatcag agagatataa gggcaggaat atagtgtatt
     9421 tatccaatta tttatccgag gatcacgagt tatataagat gactggaagt gaattgtttg
     9481 atacctatct acctcactta aaaaaaatta accccgattt cacagccgac tgggtcaaag
     9541 agtatcatca ccataaaata cctaacgccc aaccagtagt tgacacaaat tacggcaaaa
     9601 atattcctgc tcacgagact gggatccaaa acttgtattt ggcaaatacc tcacaggttt
     9661 atccacaaga cagaggtacc aattacagcg ttgcaatggg tcgaaaaatg gcccatttgg
     9721 ccttattaaa tctcaaaaac gaatgaattg tccgagaaag gtccctatga tatgcttgtg
     9781 acaattttta caaagatgag ggtaaccatc tgtgacaaca gataaagaca acctcaggac
     9841 taagattcaa gcggccattc agtcccaaaa aaggcctact gtcactgtac tatcttcctt
     9901 gctggcgatc caggattcga ttggatacat tcctgaagaa gcaatagaag aggtagcggt
     9961 taattctaat acctcaatta attcagtatt tggtgtagcc tccttctacc ctaattttag
    10021 atttacacct cctggtgaac actctgtaga ggtctgttgg ggccctactt gtcacttaaa
    10081 aggagcgacc gaagttttgg agtcggtcct ggaaaaatta gggttagatt cagaaggtga
    10141 cactgacgat ggtaaagtgt cttttcggtt taatacatgc cttggggcct gctcacaagc
    10201 ccctgtaata agtgtagatc acgaactata cggtaatgct aaacccgata ttattccgaa
    10261 tttgctgggt agtctccatg aaaaagaaaa ccaccatcag taggccttga tagtccaatg
    10321 aaatacgaag aaataacatc tcaagcaaca gcagaatggg ctgacatgac tagcggcaga
    10381 gttcctttga tcaggatagg aacggctatg tgtggtcacg ccgctggtgc tttcagggtg
    10441 gtaaaggcat tgcgaaaatg tttagaatca aaaggcatag aagccaatat acaagaggtt
    10501 gggtgcttgg gactttgtta cgctgagcct ttgttggata taaaaaagga aggtaaatca
    10561 aggcttttct tcaacaatgt aaccccggaa gaagttgaat atatagtaga tgagtatttg
    10621 ctaaaggaag gatacccaaa agaaaaagtt tttgggtata ttggcgaaga agggccagtg
    10681 agtggtgaag attcactaga gatgctcccg gggctgaaac ttcaacacag aatagccctc
    10741 agaaatgcag gacacacttc tccatacgat ataaaccagt acatagccaa tggaggatac
    10801 tccggtcttt acaaggctct tactgaaatg tctccatcag aggttataga cgaagttaag
    10861 aattctggcc ttagagggag gggaggggcc gcgttcccta caggggtgaa atggagtttt
    10921 ttggttggat cacccggacc tacaaaatac atattgtgca actgtgaaga gggagacccc
    10981 ggtgcctata atgacaaagg catcttagaa agcgatcctc acaccttgct agaaggcctt
    11041 gcgatagccg gttatgctac tggggcatcc aatggaatcg tcttcattcg gcacggccat
    11101 gatggcccca ttgaaagaac tgagaaagct attgaacagg cctacgatct agggctaatt
    11161 gggaacaata tattaggtac agattttagt tttgatatcg aagttgcctt aaccggcgaa
    11221 tcttatgtag cgggtgagga aactgccctt atggaagcag tggaaggaaa aagggcgatg
    11281 cctagattca gaccaccgtt ccccgcagct ttcggggttt ggggaaagcc aagcaacatt
    11341 aataatgtta aatcactttc gtatgctcct gagattatct caaaaggagc tgactggttc
    11401 tcctccattg gtgtcaatca gagcaccgga actgctattg tatgcctcag tggcgatgtg
    11461 aatcgccctg gattatatga ggtaccgatg ggattgaccc ttggtgaagt gatagatgat
    11521 ctgggtggag gcgtcaccga caacgctcaa cttaagctct tgcagacagg cggacctctt
    11581 ggaggagtgt tagggccaga cagtaaggat attcatatag attttgacga aatgagaagt
    11641 gccggagcta ttttcggatc cgggggaata attgtcggaa gtgacaaggt ttgtgctgtt
    11701 gatttaacta gaattctggt ggctttctgc caatttgaat cctgcggtaa atgtttccct
    11761 tgtcggttag gtatggaaca cctactggaa atcgtagaaa gaatatcaaa ctgtgatagt
    11821 aaacctggcg atatggactt gatgaaaagt attggcggca cgatggaagc cggatctctt
    11881 tgtggacatg gccaacttgg tttcggtcct ataaggtctg ctatcaccca ttttgaagaa
    11941 gattttcgac tgcacataga agaaaatagg tgtccaacag gcagctgtga ccgattgaaa
    12001 atagtaccga aaaataccag accctacgct actgaccaca tagccggaga gtgatatgtc
    12061 agataaagtt aatctcacaa tagacggagt ggaaatacaa gctgagccag gtagcatgat
    12121 aattcaagct gccatggata acgaaatgta cataccatac ctgtgttact acccagggat
    12181 gaagccctac ggagcctgcc gaatgtgcgt ggttaaagca gaagccccta ctccagatgg
    12241 tagttataga gcactgcccg gaagcccagc ttcttgcaca acccctgtct ctgaaggaat
    12301 gcgagttgaa acaaatacag gagatttagt tggtcttcgt aaaggaatca tggaattgct
    12361 aatatcggaa catcctcatg gttgtttaaa ttgccacaga attgaacttt gcggtcctac
    12421 tgatgtatgc cttagacatg tttcagtgaa cgacagatgt gtcacatgcc caaaaaatga
    12481 aagatgtgag ctaaaagaca ctgttagata catggaaatg gagttgacca ctccgctgac
    12541 atacaacaac agacatcttc ctttggaagt gaaagacccg tattgggata tggacatgaa
    12601 tttatgcatc gtgtgctcca gatgtgtaag ggcgtgtgat gaagtaaggg gcgaccgagc
    12661 gattacctta atggacaagt ccggcaggag cataattggg accgcagctg gtacttcctt
    12721 gcttgagtcc gggtgcgaat tttgtggggc atgtattgat gtctgcccta ccggagcaat
    12781 cgttgagagg gattacaaat gggataaagc ggtacaaaaa gtttctacga cctgccctca
    12841 ttgccctgtt ggttgccagt tagatcttga tgtcaataaa cgaaataaag tagtgaggtc
    12901 tattcccaac cgacaggcag aggccaatat gggtatggac tgctttaaag gcaagttcgg
    12961 attggatttt gttaataaca agaa
//
LOCUS       DASCNS010000226        15702 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001766042, whole genome shotgun sequence.
ACCESSION   DASCNS010000226 DASCNS010000000
VERSION     DASCNS010000226.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 15702)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15702)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15702
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001766042"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="QF838_08185"
     CDS             complement(<1..790)
                     /locus_tag="QF838_08185"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF025065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HJO60694.1"
                     /translation="MGKVNYDDLASEVITSSATYPVWVGWGIINNIGERVKAVHDTNV
                     AYVVADEGVNSHARRVQISLESVGIPTFMFFVPAGESHKNLETVKHIYSWLADHKAER
                     GHMIVAVGGGVIGDLAGFVAATYLRGMPFAQVPTTLLSMMDASIGGKVAVDMEQGKNL
                     VGSFYQPKFVMSDVETLNTLPVRELTSGWAEAIKHGLILDESLLKLFESKHSEIAALD
                     PEIATEIIRKSVAIKARVVSQDEKETLGIRILLNYGHTVGHAIES"
     gene            complement(778..1314)
                     /locus_tag="QF838_08190"
     CDS             complement(778..1314)
                     /locus_tag="QF838_08190"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HJO60695.1"
                     /translation="MSHNIYLTGFSGSGKSTVGKALSFLTGQEFVDIDLMIEEIEQKP
                     IPDIFNCEGEEYFRAVETDCLKKISNRTNLIVSTGGGLAVDPVNVEAMENSGCIIWLK
                     ASPETILERLNIQNENTGIDNDRPMLISEEPLERIKKLLDSRQDAYKRSHAIVETDRK
                     NVEVVAEEIHRRLVNWEK"
     gene            1514..2218
                     /gene="rph"
                     /locus_tag="QF838_08195"
     CDS             1514..2218
                     /gene="rph"
                     /locus_tag="QF838_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HJO60696.1"
                     /translation="MRLDNRTDYQLRPVHFEKNPLKFSQGSVLISTGLTKVICTATVE
                     EKVPGFLAGSGKGWVTAEYSMLPGSTSPRVSRDNQRKGRSQEISRLIGRSLRASINLQ
                     ELGEITITIDCDVIQADGGTRTASITGGFVALSLAVKQMLDSGSLDALPTITPIAATS
                     VGIFGDKVLVDLCYEEDSKADADFNMVMASDGGIVEIQGSAEGNRFSRKLVDQVLDAG
                     VDAISKLFELQFKALE"
     gene            2226..2705
                     /locus_tag="QF838_08200"
     CDS             2226..2705
                     /locus_tag="QF838_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60697.1"
                     /translation="MNKASILVCIAIGLILVTSVIALNIGNKPSKMPEGSPARIVQIF
                     LEYISSENYESAYNFLSIELKKTCSFTDFAADSYNEKARYSRSTFKHLTTHLDDLESV
                     VLVEVIEVEPAFPIGTSENSYQEKFNLVKENTNWKISQLQFPINCLMTLENPDKSND"
     gene            complement(2835..3131)
                     /locus_tag="QF838_08205"
     CDS             complement(2835..3131)
                     /locus_tag="QF838_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60698.1"
                     /translation="MMSEIELIDMAKDTDKHRRRYAILIPEIISSEFFSTSRLIFCQY
                     SIHSGPPITSSDIPKAARSVLVSSRDGETKKPIKCTDDCSNPFTSIDRPTPEII"
     gene            3556..4503
                     /locus_tag="QF838_08210"
     CDS             3556..4503
                     /locus_tag="QF838_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60699.1"
                     /translation="MISSTMLLMTLFGKIFQIDEMDGWRFILISSISTLSTGAAVLLY
                     HTKYVPNTYQMTDLLKKPDAALIFSLAFLGLGFLSAGFSVLIHSVMISLLQLSWTDIV
                     QPNDFWKGPLSGGISLAMVGCLVWYTSWSRITVQTGSFNEKIAYSRIYFMVVLGISII
                     ATAGAMAGILFILVKYSLANDLGLQTIESLITPLSLAMVLGVVAIYHRQIFKDIPLES
                     KPAIQPQLQSSQTARKSVTIFSQADNSKLIKSLQERLEYDVEEIVWTTESNFDGKGIK
                     ANIDDLYNSIVKLEAGNILLIQSGQEYKIYTYKRNSLEG"
     gene            4509..5372
                     /locus_tag="QF838_08215"
     CDS             4509..5372
                     /locus_tag="QF838_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024118.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO60700.1"
                     /translation="MATIHILGAGTPTPTPDRFGSSFALEIDGDQIMIDCGPAATHKL
                     VKAGLWPTEIDYLFFTHHHFDHDIDYPCFLLCRWDQSIGNENILKVFGPNLTEKITTG
                     ILDESIGLFAHDWIARVSHPLSQRIHVNRGGTLPRKPPQVEAIDIEPGPVFKATNWSV
                     NSASAKHVQPYLDSLAYRFDTVDGSIVFTGDTEPCSSVIELAKDADLMLCMCWDEQSN
                     MSDNGENEGQCGTTGAAEMAQAANVKKLALVHIGPNLSDPAVMNSAKSDISDIFSGEI
                     IFANELDKLYL"
     gene            complement(5380..5880)
                     /locus_tag="QF838_08220"
     CDS             complement(5380..5880)
                     /locus_tag="QF838_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024698.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO60701.1"
                     /translation="MRIREKRVEDIRNEYSWRVDPELSRLDATRPMTMSYEDFFRYSK
                     EEMQFLNYRSKRLAVETLGGVHIGNIMYYDLNMQNSQAELGIMIGDKDYWSSGYGTDT
                     VNTLLRHLFTTLELDRVYLHTLSWNYRAQASFAKSGFKLVRNVKRGGQDFILMEVLRS
                     DWEEPI"
     gene            complement(5915..6343)
                     /locus_tag="QF838_08225"
     CDS             complement(5915..6343)
                     /locus_tag="QF838_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HJO60702.1"
                     /translation="MNLVSAGGIVYREDAGSILVVVCGRNCPTSFNLPKGTPEPNETK
                     EQTALREVEEETGLKVKLGKYVGSVSYWVNNHPDYPDQQLYKTVYYYLMEPIGGDFSR
                     HDGEFDTVDWVDVREILNTLSFENEVKIVQKGISLVKGRK"
     gene            6618..7184
                     /gene="pyrR"
                     /locus_tag="QF838_08230"
     CDS             6618..7184
                     /gene="pyrR"
                     /locus_tag="QF838_08230"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HJO60703.1"
                     /translation="MYNPSATSTNMSVKKIQTDVQMKRALTRISHEILERNSGAKDLI
                     LVGMHSRGVPLAFRIRNLISSYEGQTVDVGSLDIGLYRDDISEKPQATMKPTDISVDI
                     NGRIVVLVDDVLYTGRSIRAAMDAINDLGRPSQIQLAVLIDRGHRELPIRADFIGKNV
                     PTSQEEDVAVKLAEIDGLDEVIISSPIT"
     gene            7197..8195
                     /locus_tag="QF838_08235"
     CDS             7197..8195
                     /locus_tag="QF838_08235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HJO60704.1"
                     /translation="MTTNDTNTRKSIRQKHILDLDALDQSDIELIFENTDSMMEVLNR
                     GIRKVPALRGKTIITLFYESSTRTRVSFEQAGKILSADVINVSSDGSSTKKGESLYNT
                     ALTLQAMKADAIVIRHQHSGAAYFLAQQMESTSIINAGDGMHAHPTQSLLDLYTIKKH
                     LGTISSKKVVIIGDLLYSRVARSNLWGLTTMGANIVLCGPNTLLPQDFQDNRLLETNH
                     PFRNIQIEENIGTALEDADVVMALRLQSERQKAGHLPSLREYSRRFGITSERLKVAKP
                     DAIIMHPGPMNEGVEIESDVAHGPQSVIESQVTTGVAIRMAILYDLITGNHSEKEL"
     gene            8197..9510
                     /locus_tag="QF838_08240"
     CDS             8197..9510
                     /locus_tag="QF838_08240"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HJO60705.1"
                     /translation="MAANVLIKGGRIIDPSQSIDEISDLLVTDGIITEIKTSIDTPRN
                     SDVFDAEGKIVCPGFIDLHCHLREPGEEYKETIQTGTAAAAAGGFTTVCAMPNTIPAM
                     DNQSVIEYVLKKARETAQIRVLPIGSVTIGRKGKQLSEMSELAELGVIGFSDDGDPVE
                     DPNIMRQALSYSAGLNLPIINHCEVPALAEGASMNEGWVSNRLGLKGMPNSAEEVMVA
                     RDLSLAELTGGRIHIAHASTVGTLNLIKAAKARGIKGTCEVTPHHLTLSERLVMGPTE
                     DSFVPLARNAYDTNAKVNPPLRNQSDVSAMIEGLQNGTVDVIATDHAPHNEVDKTTTF
                     QDAAFGISVLETAFGSLMTLVDNGHIDITTLIKKLTADPAKFLGMGIGNLKKGSNADI
                     TVFDPSETWIINSQTFLSKGKNTPLEGTLLKGKIKATFYKGRKIY"
     gene            9531..10634
                     /gene="carA"
                     /locus_tag="QF838_08245"
     CDS             9531..10634
                     /gene="carA"
                     /locus_tag="QF838_08245"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HJO60706.1"
                     /translation="MATKAFLILEDGSIYPGESFGYKGETYGEVVFNTSMTGYQEILT
                     DPSYAGQIVVSTYPLLGNYGITSTNIESSKIQVSGYVVRQYCEEPSHTKITETINEYL
                     DNQEIVGISEIDTRAVTRKIRSKGVMMGAIAVDMSPEQALQQLKNKPSYDEQNFVQTV
                     SANERYRWMEAGLGYDPIADNLRILVSDYGLKYNILRILKGKGCDVEVFPSSATSDDL
                     LTENPDGILLSPGPGDPELLDHLVSTTKDILGKIPVMGICLGNQILAKSLGAQTYKLK
                     FGHRGANHPVKDVASERIYITAQNHGYSIDPESLPSDVDITHISMNDYTVEGIRHKSL
                     PALSIQYHSEASPGPKDNEYIFDDFLEMVKETK"
     gene            10637..11389
                     /locus_tag="QF838_08250"
     CDS             10637..11389
                     /locus_tag="QF838_08250"
                     /inference="COORDINATES: protein motif:HMM:NF015000.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HJO60707.1"
                     /translation="MRRKITNIAHRGASSYAPENTHAAFDMAINMGVKHIELDVHSSK
                     DGHLVVIHDDTLNRTTNGNGNVTDYTLKELKALDAGSWFDHKYSGERLCTLEEILVEY
                     KSAVHLHVEIKGNTPNLAQKTCDMIRSFKAIEKTIITSFWMPRLVETREYAPEIKTGW
                     LVPLGPGSEWNDSIISDSLNYGFDQVCPRADLVTPSLVTKLHDKGFEVRCHGVLTEAL
                     MENVVECGADGMTINFPDKLISYLHKRGLAID"
     gene            11382..14735
                     /gene="carB"
                     /locus_tag="QF838_08255"
     CDS             11382..14735
                     /gene="carB"
                     /locus_tag="QF838_08255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HJO60708.1"
                     /translation="MTDTPKKILVIGSGPIVIGQAAEFDYAGTQACKSLREEGIETIL
                     VNSNPATIMTDADVADSVYIEPLTVPVISRIIEREQPDAVLPTLGGQTGLNLAVELAE
                     AGVLDQYGVRLLGSSVDSIKKAEDRQLFRELLTDIGEPTPPSFVVNTYEEAAEAKNTI
                     GLPLVIRPAYTLGGTGGGIAEDDDTFTEIVKSGLSASPIHQVLVEKSIVGWKELEYEV
                     MRDSSDNCITICNMENIDPMGVHTGDSIVVAPSQTLSDNEYQMLRTASLKIIRELGIE
                     GGCNVQFALAPGNVVGETLPDAGRTGPSYYVIEVNPRVSRSSALASKATGYPIARVAA
                     KIAIGMTLDEIQNAVTQKTVAAFEPALDYCVVKIPRWPFDKFPSGERTLGTQMKATGE
                     VMAIDRTFEAAFQKAVRSLEISNRSIMWEDPVWFTDEESVLNKLPIYPNDQRLWALLA
                     GLRKNVSADELSRRTGIDPWFTRSFSKIIAMERTIDSNDLSYDLLFKAKRLGFTDQRI
                     GEIVNQSAKDIRKLRKRLNIQPVYKMVDTCAAEFEAQTPYFYSTYETENEAEPLPGKR
                     ALVLGSGPIRIGQGIEFDYCSVHAAWALQEEGVKSILVNSNPETVSTDFDTSDRLYFE
                     PLDEESIFDIIDNERLATDSEHGENPPSIVQFGGQTAINLSQTLAKESMPILGSTAHT
                     IDVASDRHLFEEFLRGIDIPNPPGGSVSNIQEALQVANEIKYPVLVRPSYVLGGRAME
                     IVQTPEELQRYMNDALEAGEGRKVLIDKYFVGKEVEVDAVCDGESVLIPGIMEHVERA
                     GVHSGDSMAIYPGLTLTKQEIATITDYTTRIGKSLNIKGLMNIQYVIVGGNTYRSPGS
                     ITEGTAEYEKTEIFVIEVNPRSSRTIPFISKVTGVPMVNLAVKAMLGKKLSDMGYGTG
                     LFSTHGIVGVKAPVFSMSKLTGVDTYLGPEMKSTGEVMGIDYDYESAISKALIAADLR
                     LPVNGSILLSISDEDKKQSGKLIQKLVDEGYDLFATEGTASLISELGKKVVAIPKRLN
                     EGHPNVVDIIVNGTVDAVVNTITGNSSAVKDGFHIRRAAVEKRIPCFTSIDTATAAAE
                     SLTSEGPNYNVKSMLEYIPTPEGEPHHAK"
     gene            14725..15234
                     /locus_tag="QF838_08260"
     CDS             14725..15234
                     /locus_tag="QF838_08260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255440904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:14938..14940,aa:Sec)
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HJO60709.1"
                     /translation="MPSDIPQSTVGSELPKNVFKSTVSVECEHLEKMLHAGKKDKFTF
                     YSDEPPRLGGDDNYPSPLTYVVGGIGFULLTNLKRYASMKEIGIHSPKVHVEMDYYLK
                     GSIADNTVENGVTEVRSFFDVESDHSEEDLIEVIQLAKKGCFAESLVKTTVPLKSICT
                     LNGTEVNIA"
     gene            15262..>15702
                     /locus_tag="QF838_08265"
     CDS             15262..>15702
                     /locus_tag="QF838_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024867.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HJO60710.1"
                     /translation="MIPLLLTVKNFMCYGEDVPSLNLEPVHIACISGDNGYGKTALLD
                     AITWSIWGKSRARTQDELVNIARNSMFVELDFFAGDNRYRVTRTYAKSKGASSGKSEL
                     NLSIIVGDTVTSLMANTIKETEEKIVDLLNMDYETFINTSYLKQG"
BASE COUNT         4942 a         3289 c         3274 g         4197 t
ORIGIN      
        1 tagattctat agcgtggcca actgtatgac cgtaattgag gagtattctg atccctaacg
       61 tttctttctc atcctgcgaa accactcgtg ctttaattgc cacacttttc cttatgattt
      121 cagtagctat ttcaggatct aatgccgcta tttcgctatg tttgctttca aatagtttca
      181 gcaaggattc atccagaatt aacccgtgtt ttatagcctc tgcccatcct gaagtcaatt
      241 ctctaacagg cagtgtattt aaagtctcta cgtcagacat tacaaacttt ggttgataaa
      301 aagagccaac cagattttta ccttgctcca tatcgacggc aactttcccc ccaatggagg
      361 catccatcat ggaaagcaga gttgtgggta cttgagcaaa gggcatccct ctcaaatacg
      421 tggcggctac aaatccagcc aaatcgccaa ttactccccc acctactgct actatcatgt
      481 gccctctttc agccttgtga tcagccaacc aagaatagat atgcttaacc gtctctaaat
      541 tcttgtggct ttctccagca ggaacaaaaa acataaatgt tggaattccg accgattcca
      601 acgaaatctg cacccttcta gcatggctgt tgacaccttc atcagccaca acgtatgcca
      661 cattagtgtc atgaacagcc ttaactcttt ccccaatatt attgattatg ccccacccta
      721 cccatacagg atatgtagcg cttgaggtga ttacctctga tgccaaatca tcgtaattta
      781 cttttcccaa ttcacaagtc tcctatgaat ttcttcagcc acgacctcta catttttcct
      841 atctgtttcc acaatagcat gagatctttt ataagcatcc tgccgactat ctaacaattt
      901 cttaattcgc tccaagggtt cttcagaaat aagcataggc ctatcgttat caattcctgt
      961 gttctcattt tgtatattta gtcgttcaag tatcgtctct ggagaagcct ttaaccagat
     1021 aatacaacca gaattctcca ttgcctcaac attcacagga tcaactgcga gtcctcctcc
     1081 tgtagataca attaagttag tacggttgga tatttttttt aaacaatccg tttcaactgc
     1141 tctaaaatat tcctcacctt cgcagttaaa gatgtcgggt atgggttttt gttcaatttc
     1201 ctctatcatc aagtctatat ctacgaattc ttgacccgtc agaaaagaaa gagcttttcc
     1261 tactgtagat ttacctgaac cagaaaagcc tgtcaggtat atattgtgac tcataaaccg
     1321 aactctacct cctttacgac aaaaatcttc aaccagtcta caacagaagc aatagtagag
     1381 ataacaacga aaaagaaaga cgtgttattt tcctgttatc cctttaaatt acggttttaa
     1441 aaatcttcct aacagtttag tgacaatata aaatcaactg atataaacat tgaaagttac
     1501 aaaggtatag aacttgaggt tagacaatag aaccgactac caacttagac ctgtgcattt
     1561 tgaaaaaaac cccctcaaat tttcacaagg atctgtgctt atttccacag gtttaactaa
     1621 agttatatgt accgcgaccg tcgaggaaaa agttcctggc tttctggcag ggtcaggcaa
     1681 aggctgggtt actgcggaat attccatgct accaggctcc acctctccac gagtgtcacg
     1741 tgacaatcaa aggaagggaa gatcccagga aatatccaga ttaataggaa gatctcttag
     1801 agcgtccatt aatttgcaag aacttgggga gatcactatc acgatcgatt gtgatgtcat
     1861 tcaagctgat ggaggaacca gaacagcctc aattaccggt ggatttgtgg cattaagctt
     1921 agctgtgaaa caaatgttag attcaggatc tcttgatgct ttacccacga taacgccgat
     1981 agcagctaca agtgtgggta tatttggaga taaagtgttg gtggatttat gctacgagga
     2041 agattccaaa gccgatgccg atttcaatat ggtaatggcc tcagatggcg gaatagttga
     2101 aattcaaggc tcagcggaag gtaacaggtt ttcaagaaaa ctagtagacc aagtcctcga
     2161 cgcaggagtt gacgcaatat caaaactttt tgagctgcaa tttaaggcat tagaataatt
     2221 gtaaaatgaa caaagcctcg attttagttt gcatcgccat aggattaatt ttggttacct
     2281 cagttattgc attaaacatt ggtaacaaac catcgaagat gcccgaagga tcaccagcga
     2341 gaatagtcca aattttccta gaatatattt ccagtgagaa ttatgaatcc gcatacaatt
     2401 tcttgtctat tgaactcaaa aagacatgta gtttcaccga cttcgccgct gacagctata
     2461 acgagaaagc ccggtactca cggtcgactt tcaaacacct aaccacccac ttggacgatt
     2521 tggaatccgt ggtgttggtg gaagtaattg aagtggaacc agccttcccg ataggaacgt
     2581 cggaaaattc ttaccaagaa aaatttaatc ttgtgaaaga aaatacgaat tggaagatta
     2641 gccaattaca atttcctatt aattgcctta tgactttgga aaaccctgac aaatccaatg
     2701 actgaggcag tcgatgtcct gatcttcttc ccattaatca tattaataaa tctttgtatt
     2761 tataagctaa tctcttttag aaaaggtggc aattatggtc aatcattggt tgatcctgcg
     2821 aggcgactat atctctatat aatttccggg gttggcctat cgatgcttgt gaatggattg
     2881 gaacaatcgt ccgtgcactt gataggtttt tttgtttctc catctctgct agagacaagt
     2941 acagaacggg ctgctttggg aatatcactg ctagtgatag gggggccgct gtggatactg
     3001 tattggcaaa aaattaaccg agaggtgctg aaaaattcag atgaaattat ctcaggtata
     3061 agaattgcat atcttcttct gtgtttgtcg gtatctttcg ccatatcaat aagttctatt
     3121 tccgacatca tttcagagac cttccatgca tcagaattta actggcctcg aatacctacc
     3181 ctagtaatct ggttattggt ttggagctac catttgaagg tactgttgca gaccaagccc
     3241 tcgaatcatg taaatagcct aagtcttcgt aacacatttg tttacagctt tagctttatt
     3301 ggattattta tacttgcttc aagtttgagc agcttgattt acctcgcagt ctttaacatt
     3361 atccaaaaca ctgttggcaa tggggaaatt ttaattttca acggtagcct cagctcatta
     3421 aggttaacta tgtcagtact aataggtttc ggtacatggt ctgtccattg gctgatcctg
     3481 aggcataaat tagtaaagca agaatatgaa ccgatctact tatatctaat ttatacattt
     3541 acgatagtgt ctttaatgat atcaagcaca atgctgctaa tgactctatt cggtaaaatc
     3601 ttccaaattg atgaaatgga tggatggcga tttatactca tttcctcaat aagtacgttg
     3661 agcaccggtg ccgctgtttt gctttatcac acaaagtatg ttcccaatac ataccaaatg
     3721 actgatttat taaaaaaacc tgatgcagca ttaatattct ctttggcgtt tttggggttg
     3781 gggttcttgt ctgcaggatt ttcagtccta attcactcag tgatgatctc tctactacaa
     3841 ctgagctgga ctgatatagt ccaacctaat gatttttgga aaggaccttt atccggaggt
     3901 ataagtttgg caatggttgg ttgcctcgtg tggtatacct cctggagtag aataactgtc
     3961 cagactggca gttttaacga aaaaattgcc tactcccgca tctatttcat ggtagtccta
     4021 ggaataagta ttatagccac ggcaggcgcc atggcaggca ttctatttat cctcgtgaaa
     4081 tattctcttg ccaacgacct cgggctccag acgattgaat ctttaatcac acctctttcc
     4141 ctcgcaatgg tcttaggggt cgttgcaatt tatcaccgtc aaatattcaa agatatacct
     4201 ctggaatcaa agccggcgat tcaaccccaa ttacagagct cccaaactgc aagaaagagt
     4261 gtcacaatat ttagtcaagc agataacagt aaattaatca aaagcttgca ggagcgttta
     4321 gagtacgacg tggaagaaat cgtgtggacc accgaatcaa attttgacgg aaagggcatc
     4381 aaagcaaata ttgacgattt atacaattca atagtcaagc tagaggctgg taacatactt
     4441 ctaatacaat ccggccagga atacaaaatc tatacttaca aaagaaactc cttagaaggt
     4501 tgatacaaat ggcaacaata catatcctag gtgctggtac gccgacacct acacccgaca
     4561 gatttgggtc atcattcgct ctagaaatag atggagacca aataatgatt gactgtggtc
     4621 cagcggccac gcataaatta gttaaagccg gtttatggcc cacagaaata gactacttgt
     4681 ttttcacaca ccaccatttt gatcacgata ttgattatcc ctgcttcttg ctgtgtagat
     4741 gggatcaaag tataggaaac gaaaatatcc tgaaagtatt tggccccaat ttaaccgaaa
     4801 aaataacaac gggaattctt gatgaatcta taggattgtt cgctcacgat tggatcgcac
     4861 gggtcagcca cccgctaagc caaagaattc atgtaaacag aggcgggaca ctaccaagaa
     4921 aacctccaca ggtagaagcg attgatatcg agccgggtcc agtatttaaa gcgaccaatt
     4981 ggagtgtcaa cagtgcttct gcaaagcacg tgcaaccata tttggattca ctggcatacc
     5041 ggtttgacac ggtggacgga agcattgtat tcactggtga taccgaaccc tgcagctcag
     5101 tcattgaatt agccaaagat gcggatttga tgctttgtat gtgttgggac gaacaatcca
     5161 acatgagcga taatggagaa aatgaagggc aatgtgggac aactggagcc gcagaaatgg
     5221 ctcaggccgc caatgtgaaa aaattagcgt tggtacacat aggtccaaat ttatccgacc
     5281 ctgccgtgat gaattcggca aaatctgata ttagtgatat tttctctgga gaaattattt
     5341 ttgccaatga attggacaag ttatatcttt agatcaaatt caaattggtt cttcccaatc
     5401 agatcgcaga acttccataa gtatgaagtc ttgcccgcct cttttaacgt tacgaaccaa
     5461 tttgaaaccg gatttggcga aagatgcctg cgctctgtag ttccaggaaa gcgtatgaag
     5521 ataaacccta tctaattcca aagtagtgaa caaatgtcgc aacaatgtat taaccgtatc
     5581 ggtaccatac ccgctactcc agtaatcctt atcgccaatc attatcccta attcagcctg
     5641 actattttgc atatttagat cgtaatacat gatatttcca atatgaacgc cccccaaggt
     5701 ttccactgcc agtctttttg agcgatagtt tagaaattgc atctcttctt tcgaatacct
     5761 aaaaaagtct tcgtacgaca tagtcatagg tcgggttgcg tctaatcggg aaagttcagg
     5821 atccaccctc cacgaatatt cgtttcggat atcttccaca cgtttttccc gtatacgcac
     5881 cttttccccc tcctcattaa caagggggag ttccttattt tcgtcccttg accagggaaa
     5941 tgcccttctg aacaattttt acctcgtttt caaatgaaag agtatttaat atctcacgta
     6001 catctaccca atctacagta tcaaattctc cgtcatgcct agaaaagtct ccccctatag
     6061 gttccatcaa ataataatag accgttttat ataattgctg gtctggataa tcaggatggt
     6121 tattaaccca ataacttact gagccaacat atttgccaag tttgactttt aatcccgtct
     6181 cttcttcaac ctcccgcaaa gcagtctgtt ccttcgtctc gttaggttct ggggtacctt
     6241 tgggtagatt gaaggaggta ggacaattcc taccacaaac cactacaagg atagaaccag
     6301 cgtcttctcg ataaactatt ccgcctgctg ataccaagtt cattctaagt acattgtaaa
     6361 ccaaagaact ggggatataa cacggtacat gtatctgtag agttgtaagt agtgaaatcc
     6421 ttcgtgtaac tttaactttc gccattatga gtgtatattt attggtgagt gggcgcattc
     6481 gctcactgca ccctttaagt cagtccagag agtctgccaa gggagcgaat gcttcgagtt
     6541 accaagttgg ggtagcccgt ttgcgaaatg cctcttgctg acctgggcgg aggttttttt
     6601 ttggaaaatc taatttcatt tacaacccca gtgcaacatc aaccaacatg tctgtaaaaa
     6661 agatacaaac agatgtgcaa atgaaacgag cattaacaag gatttctcac gaaatacttg
     6721 aaagaaactc aggggcaaag gatttgatcc ttgttggtat gcattctaga ggagtacctc
     6781 tagccttcag aattaggaat ttaatatcca gctatgaagg tcaaactgtg gacgtcggtt
     6841 cattagacat tggtttgtac agagatgata tttctgaaaa accacaggca actatgaaac
     6901 cgactgatat ttctgttgat attaatggcc gaatcgttgt gttagttgat gatgttttat
     6961 acacagggcg gtcaataaga gctgcaatgg atgcgatcaa cgatttgggt aggccaagtc
     7021 aaatacagct agccgtccta atcgataggg ggcatagaga gcttccaata cgagctgatt
     7081 ttatcggcaa aaatgtgccg acttcacaag aggaagatgt ggcggtgaag ctagcagaaa
     7141 tcgatggcct tgatgaagta ataatctcga gcccaatcac ataggtcaga acgaaattga
     7201 caacaaacga caccaatacc agaaaatcca ttagacaaaa acacatctta gatttagatg
     7261 cattagatca atccgatata gagcttattt tcgaaaacac ggattctatg atggaagttc
     7321 taaacagagg tatcaggaaa gttcctgctc tcagagggaa aacaatcatt actcttttct
     7381 acgaatccag cactcgcacc agggtatctt ttgaacaagc tgggaaaata ttgagcgctg
     7441 atgtcataaa tgtctccagt gacggtagta gcactaaaaa aggggagtct ttatataaca
     7501 ccgcactgac actccaagct atgaaagcag atgcaattgt cataaggcac cagcattccg
     7561 gtgccgcata tttcctagca cagcaaatgg aatcaacgag cataatcaac gctggagatg
     7621 gaatgcatgc ccatcccaca caaagcttac tggacttgta caccatcaaa aaacatctcg
     7681 gtactatatc ttctaaaaag gtcgtcataa ttggcgattt gctctatagc cgggtcgcta
     7741 gatcaaattt atggggactt acaactatgg gagcaaatat tgtcttgtgc ggaccaaaca
     7801 cgttactacc tcaagatttc caagacaatc gccttcttga gactaaccac ccatttcgaa
     7861 acatacaaat tgaggagaat ataggtactg ccctagaaga tgctgatgtg gtgatggcac
     7921 tgaggctaca gtctgaaaga caaaaggcag gccatttacc ttctttgaga gagtattcta
     7981 gaagatttgg cataacctcg gagcgcctaa aagttgccaa acctgatgcg atcattatgc
     8041 accctggacc catgaacgaa ggggttgaaa ttgaatctga tgtcgcacat gggcctcagt
     8101 cagttattga aagtcaggtt acaacaggag tggcaatcag aatggcaatc ctctacgacc
     8161 tgataactgg gaatcattca gaaaaggagt tataacttgg cagcaaatgt cttgataaaa
     8221 ggtggcagaa ttattgatcc ctctcagtca attgatgaaa tttctgacct actagtgaca
     8281 gatggaataa taaccgaaat caaaacatcc atcgatacac cgagaaactc tgacgttttt
     8341 gacgcggaag ggaaaatagt gtgtccgggg ttcattgacc ttcattgtca tctaagagaa
     8401 cccggagaag aatacaaaga aacaattcaa accggaacag cagcagcagc agcaggcgga
     8461 ttcaccactg tatgcgctat gcccaatact attccggcaa tggataacca atccgtaatt
     8521 gaatatgtac ttaagaaggc aagagaaacg gctcaaataa gagtacttcc catcggttct
     8581 gtaactatag gccgaaaagg taaacaactc agtgaaatgt cggaactagc agagctcgga
     8641 gtcataggct ttagcgatga tggtgaccca gtagaagatc ccaacataat gaggcaagct
     8701 ctcagttata gtgctggttt gaatcttcca atcatcaacc attgtgaggt tcccgcctta
     8761 gcggaaggag cttcaatgaa cgaaggctgg gtgtccaata gattggggtt aaaaggaatg
     8821 ccgaactcgg cagaggaggt tatggtggca cgggacctct ctttggcaga attgactggt
     8881 ggccgtattc acatagccca cgccagcacg gtgggaacac ttaacttaat taaggctgca
     8941 aaagccaggg gcattaaagg aacttgtgag gtcactcctc atcatttgac actctcagaa
     9001 aggcttgtga tggggccaac tgaagactcc tttgttcctt tggctcgtaa cgcctatgac
     9061 actaatgcaa aagtaaaccc gccactcaga aatcaatcgg acgtaagtgc catgattgaa
     9121 gggcttcaga atggcacagt agacgtgatt gccactgacc atgccccgca taatgaggtg
     9181 gacaaaacaa ctacatttca ggatgctgcg tttggaatta gcgtcctaga aaccgccttc
     9241 ggatcactca tgacattagt agataatggt catattgaca taaccaccct cattaaaaaa
     9301 ctgacggcag atccagccaa atttttgggt atgggaatag gaaatttaaa aaagggtagc
     9361 aatgcagaca ttacagtttt tgacccctct gaaacttgga ttatcaactc ccaaactttt
     9421 ttatctaaag ggaaaaatac tcccttagag gggaccttac tcaaaggaaa aattaaagcc
     9481 actttttata aaggcagaaa aatatactaa tcgatactgg tgattgaaca ttggcaacaa
     9541 aagcttttct aatacttgaa gatggatcta tttaccccgg tgagtcattt ggatacaaag
     9601 gtgagaccta cggtgaagta gtttttaaca cttcgatgac agggtatcag gaaatactca
     9661 cggatccttc atacgcaggc cagatagtag tttcgacata cccccttcta ggtaactacg
     9721 gcattaccag tacaaatata gaatcgtcca aaattcaagt ctccggatat gtcgtgaggc
     9781 aatattgtga agaaccaagt cataccaaaa taaccgaaac cataaatgag tacctcgaca
     9841 accaggagat agtgggaatt tctgaaatag acaccagagc tgttacacgt aaaataaggt
     9901 cgaaaggagt gatgatgggc gccattgcag tcgatatgtc accagaacaa gccttacaac
     9961 agttgaaaaa taaaccatca tatgacgaac agaattttgt ccagacagta tccgccaatg
    10021 aacgttaccg atggatggaa gcaggactag gatacgatcc tattgctgac aatttacgaa
    10081 tccttgtctc agattatggt cttaaataca acatcctacg gattctgaaa ggtaaaggtt
    10141 gtgatgttga agtgtttcct tcatcagcca cttctgatga tctactcact gaaaaccccg
    10201 acggcatatt actctctccg ggaccaggag acccagaact tctagatcat ttagtatcca
    10261 ctacaaaaga tattttgggg aaaatacctg taatgggaat ttgcctcggg aatcagatac
    10321 tcgcgaaatc tcttggtgcc caaacctaca agcttaagtt tgggcataga ggagccaatc
    10381 acccagttaa agatgttgct tcggaaagaa tctacataac tgctcaaaat catggatact
    10441 ccattgatcc tgaatctttg ccatcagacg tcgatatcac ccacatcagc atgaatgact
    10501 atacagtaga aggtatacgc cataaatctc ttcctgccct tagtatccag taccattctg
    10561 aagcctcccc cgggcctaag gacaatgaat acatatttga cgatttccta gagatggtta
    10621 aagagaccaa ataattatgc gacgaaaaat tactaatata gcccatcgag gagcatcctc
    10681 ctatgctccc gaaaatacgc atgccgcttt cgatatggcg atcaatatgg gagttaaaca
    10741 tatagagctt gacgttcatt cttcaaaaga tggtcacctg gttgtgatcc atgacgatac
    10801 gctcaaccga acaacaaatg gaaatggaaa tgtaaccgat tacacattaa aagaactaaa
    10861 ggcgctagat gccggttcct ggtttgatca caaatattct ggggagaggt tatgcacttt
    10921 ggaagaaatt ctcgttgaat acaaatcagc agtccactta cacgtggaga ttaaaggaaa
    10981 tacacccaac ctggcacaaa aaacctgcga tatgatcagg tcttttaaag caattgaaaa
    11041 aacaataata acctcatttt ggatgccacg gctggtagaa accagggaat atgcccctga
    11101 aataaaaact ggatggctcg ttcctctggg acctggtagt gaatggaatg actctataat
    11161 atcggactcg ttgaactatg gattcgatca ggtgtgtcct agagccgatc tagtgacccc
    11221 atcccttgtc accaaattac atgacaaggg atttgaagtc agatgtcatg gagtgttaac
    11281 tgaggcactt atggaaaatg tagttgaatg tggagcagat ggtatgacta taaatttccc
    11341 ggacaagtta atttcttatt tacacaagag aggactggca attgactgac acaccaaaaa
    11401 agatccttgt aatcgggtca ggtccaattg taataggcca agcagctgaa tttgattatg
    11461 ccggcacaca ggcatgcaag tcattaagag aagaagggat cgaaaccatc ttggtaaatt
    11521 caaatcctgc gacaatcatg actgatgcag atgtagcaga ttctgtatac atagagcctt
    11581 taaccgttcc agtcatatcg aggattatcg agagagaaca gcctgatgct gtattaccta
    11641 ccttaggggg tcaaacaggc ctaaacttag ccgttgaact cgccgaagca ggtgttcttg
    11701 atcagtatgg agtccgctta ttaggaagca gtgtagattc tataaaaaaa gcagaagatc
    11761 gacagttgtt ccgagaatta ctcactgata tcggagagcc tactcctcct agctttgtag
    11821 taaacaccta cgaagaggct gccgaggcaa aaaatacgat aggtctacca ctcgtcatca
    11881 gaccagccta cactttagga ggaactggtg gaggtatagc cgaagatgat gataccttca
    11941 ccgagatagt taaatcagga ctgagtgcaa gccctatcca tcaggtgcta gttgaaaaat
    12001 ccatagttgg atggaaagaa cttgagtacg aggtaatgag agatagttct gataattgca
    12061 tcaccatatg taacatggaa aacatagacc caatgggggt tcatacgggt gacagcatcg
    12121 tagtagcccc aagccaaacc ctatctgaca atgaatatca gatgctgaga acagcgagcc
    12181 taaaaattat aagggaactg ggaattgaag gtggttgcaa cgtacaattt gctttggcac
    12241 caggaaatgt agttggcgaa accctacctg atgccggaag aacaggacct tcttactatg
    12301 tgatagaagt gaacccacga gtcagtagaa gttcagctct ggctagtaag gccaccggat
    12361 accctatagc gagagttgcc gcaaaaattg ccataggtat gacccttgat gaaattcaaa
    12421 atgcagtaac tcaaaaaact gtagccgctt ttgagcctgc attggattac tgtgtagtga
    12481 aaataccaag atggcctttc gataaattcc cttcaggaga aagaaccctt ggaacccaaa
    12541 tgaaggcaac tggggaagta atggccatcg atagaacctt cgaagctgcc tttcaaaagg
    12601 ccgtgaggtc acttgagata tcaaacagat caataatgtg ggaagatcca gtatggttta
    12661 ctgatgaaga atctgtattg aataaattac ccatctatcc aaatgaccaa agattgtggg
    12721 cactgctagc aggacttaga aaaaatgtga gtgctgacga gctttctagg cgtacaggaa
    12781 tagacccttg gtttacacgg tcattctcca aaattatcgc aatggaaaga accatagact
    12841 ctaatgacct ttcttacgat ttattattta aagctaaaag attaggattc acggatcaac
    12901 gaattgggga gatagtgaat caatctgcaa aagatattcg aaaattacgc aaaagactca
    12961 atattcaacc agtgtacaaa atggtagaca catgtgctgc cgaattcgaa gcccaaactc
    13021 cttattttta cagtacatat gaaaccgaaa atgaagcaga accgcttcct ggcaaaaggg
    13081 ctttggtact tggcagtgga cctataagaa tcgggcaagg aattgagttt gactattgtt
    13141 ctgtgcatgc agcgtgggca cttcaggaag aaggggtgaa gagcatacta gtgaactcca
    13201 atccagaaac agtatctaca gattttgata ccagtgatag attgtatttt gaacctttgg
    13261 atgaggaatc gatatttgac ataattgaca atgaaagatt agccacagat tcagagcatg
    13321 gtgagaatcc accctcaatt gttcaattcg gaggacaaac agcaataaat ctctctcaaa
    13381 ctctagccaa agagtctatg cctatattag gttctactgc ccacactatc gatgtcgcct
    13441 ctgaccgaca tctatttgaa gaattcttac gtggaataga tataccaaat ccgcctggag
    13501 gatcagtatc caacattcaa gaggcccttc aagtagcaaa cgaaataaaa tatccggtgc
    13561 tggtacgacc tagctacgtt ttggggggca gggcaatgga aattgtccaa accccagaag
    13621 aattgcaacg ctacatgaat gatgcattgg aggctggcga aggaagaaaa gttttaattg
    13681 acaaatattt cgtcggcaaa gaagtggagg tagatgccgt atgcgacggg gaatcagtcc
    13741 tgattcctgg aataatggaa catgtagaaa gagccggagt ccatagcggt gactcaatgg
    13801 caatttatcc tggcttgacc ctaacaaagc aagagatagc cacaattact gactacacca
    13861 ctcgaatcgg aaaatcttta aacatcaagg gactcatgaa tattcagtac gttattgttg
    13921 gtggcaacac ttacagaagc ccagggtcca taacagaagg tactgcggag tacgaaaaaa
    13981 ccgaaatatt tgtcattgaa gtcaaccctc ggtcaagtag gaccataccc ttcatatcca
    14041 aagtgactgg tgtcccgatg gtaaacctgg ccgtaaaagc gatgttaggc aaaaagctat
    14101 cggacatggg atacggaaca ggcctatttt cgacccacgg catagtaggt gttaaagccc
    14161 ctgtattctc aatgtctaag ctaacgggcg tagatacata cctgggacca gaaatgaaat
    14221 caactggtga ggtgatggga atagattatg actatgaatc ggctatcagc aaggctttaa
    14281 ttgccgccga tctcagactt cctgtaaatg gttctatact cctaagcatc tctgatgagg
    14341 acaaaaaaca gtcaggcaag ctgattcaga aactggttga tgaaggatat gatttgttcg
    14401 ccactgaagg tacggcctct cttatcagcg aattgggtaa aaaagttgta gcaataccaa
    14461 aacgactcaa tgaggggcat cctaatgtgg tagatataat cgtcaatgga acagtagatg
    14521 ccgttgtcaa tacaattacg ggtaatagca gtgccgtaaa agatggtttc cacatcagaa
    14581 gggcagcagt ggagaaaaga ataccttgct tcacttcaat tgacactgca acggctgcag
    14641 ctgaaagtct gacttcagaa ggtccaaact ataacgtcaa atcaatgctg gaatatatcc
    14701 ccacacctga aggagagcct caccatgcca agtgacatac cacaatctac agtaggatcg
    14761 gaactaccca aaaatgtttt taaatcaaca gtgtctgtcg aatgcgaaca tcttgaaaaa
    14821 atgcttcatg caggtaaaaa agataaattc accttttatt ccgatgaacc tccccgacta
    14881 ggaggcgatg ataactaccc ttctccactt acatacgtag tgggtggaat aggtttttga
    14941 ctattaacta atttgaaacg gtacgcttca atgaaagaaa taggaataca ctcaccgaaa
    15001 gttcacgttg aaatggatta ctacttaaaa ggttctatag ccgataatac agtcgaaaac
    15061 ggggtaacgg aagttagaag ctttttcgat gttgaatccg accactcaga ggaagattta
    15121 attgaagtca tccaattggc gaaaaaaggc tgtttcgccg aaagtctagt gaaaaccaca
    15181 gtaccattaa aaagtatctg caccttgaat ggaacagagg taaatatagc gtaaggtaaa
    15241 ttacaggccg gcggattaaa tatgatccca ctattgttaa ctgtcaaaaa ttttatgtgc
    15301 tacggagaag atgtcccttc tctaaatcta gagccggtcc atatcgcctg catttccggg
    15361 gataacggtt atggcaaaac tgctttactt gacgcgataa catggtcgat atggggaaaa
    15421 tcaagagcaa gaacccaaga tgaactggtc aatatcgctc gaaactctat gtttgtagaa
    15481 ctagattttt ttgcgggcga caacaggtac agggttactc gcacctatgc aaaaagtaaa
    15541 ggtgcctcat caggaaaatc agaactgaat ctgtccatta ttgtcggtga taccgtaacc
    15601 tcactgatgg ctaataccat aaaagaaact gaggaaaaaa tagtcgactt actcaatatg
    15661 gactatgaaa ctttcataaa cacttcttat ctaaaacaag gg
//
LOCUS       DASCNS010000227        11300 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001772465, whole genome shotgun sequence.
ACCESSION   DASCNS010000227 DASCNS010000000
VERSION     DASCNS010000227.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001772465"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="QF838_08270"
     CDS             complement(<1..1507)
                     /locus_tag="QF838_08270"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HJO60711.1"
                     /translation="MNGDQLMASLLKKEGTEWISCFPAQTLIEACSQEGIRPILCRQE
                     RAGVNMADAYSRIHNGNKIGVFTMQHGPGAENAFGGVAQAYADNVPMLLIPGGSTARR
                     VGVHPGFDAIPNYQHITKWAGRINTIERIPEMVSQAFTHLRNGRPGPVLLELPGDVAH
                     AEVPADMEEYQPSRSYKSYAANEDVRDIVTALLKAKAPVINAGQGTLYAEATDELIEF
                     AEIVNVPVMTTLAGKSAYPEDHSLALGTGGNTGTLMVDRFLQNTDFVLGIGTSFAISN
                     FTAPMPPEATKAQITNTAEDINRDYRVEYGAVGDAKLVLQQLIEEAKSQLGEHGRGDV
                     HGVKENIVKIKDEFMKEWGPRLNSDETPMSPYRVFNEMMKAVDPRNAIVTHDSGYPRN
                     QLVPFWPALTPRSYIGWGKSTQLGYGLGLALGAKVAAPDKIVINVMGDAAFGMSGLDI
                     ETASRSELGTITVVLNNGVMTHYYDHFPHATENWKSNELGGIYSDTAKSLGA"
     gene            complement(1540..2427)
                     /locus_tag="QF838_08275"
     CDS             complement(1540..2427)
                     /locus_tag="QF838_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013595.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HJO60712.1"
                     /translation="MPYGSGDYTYEVDQDWGRLPEGYEFNQVAGVAVDREDNVYLYNR
                     SEHQVMIFKKNGDFLKSWDVKFSNPHGMHIGPDGNFYFADRDAHVVLKYSPDQTLLLT
                     LGTYNVESNTGYSGNLMVVPHPAGPFNLPTGVVVADDGNIFVSDGYGNCRFHKFSPDG
                     ALLDTWGEAGKDNPMDFHLPHGIGLNKDGLLAICDRENHRIQFTDQDGEFMFIWDGFL
                     QPTDIAFGPDGEAYVSELQHRISILDAEGNLISRWADESSPSAGHFIAPHGIAVDSSK
                     DIYVGEVLEGRRIQKFIRQ"
     gene            complement(2533..4023)
                     /gene="tcuA"
                     /locus_tag="QF838_08280"
     CDS             complement(2533..4023)
                     /gene="tcuA"
                     /locus_tag="QF838_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HJO60713.1"
                     /translation="MTSPEKNYDVVIVGAGNAALTAALSAASKGVKVAILEKAPEHLR
                     GGNSYFTGGLFRFAYNGIEDISELLPDLTSKEKQNIDVGSYKESEFYSDVMRVTEGLS
                     DPSLLQVLTSQSFPTMLWMRNQGVPWILAYGRQAFEHEGKQRFWGGLIVEAVGGGKGL
                     SDRLFELVERENIEVLYQTSGTDLSINQEGHIEGIIAKSKSGYEKISCKSVVLACGGF
                     EANTEMRSRYLGPGWELAKVRGTQYNTGDGINMALRIGAQSHGHWSGCHAVAWDLNAP
                     PHGDRNVADLFQKHSYPFGLIVNVDGERFVDEGADLRNYTYAKYGREILNQPQRVAFQ
                     LFDQKTKHLLRDEYFIPQMTMVEAKTIEELATGLDINPGQLQITVQEFNGAVQEGDFN
                     PTALDGKHTEGITPPKSNWAQKLDKPPYLGYAVTCGITFTFGGLKIDTNTQVQDNSDM
                     PIPGLYAAGELTGGLFYNNYPGGSGLMSGAVFGKIAGENAAKEALI"
     gene            4249..4773
                     /locus_tag="QF838_08285"
     CDS             4249..4773
                     /locus_tag="QF838_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014657.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HJO60714.1"
                     /translation="MDFKVATLLKASSGHKKLESVSSTIEVDWSEFRCDVDGSVLLTK
                     TDVGVWVSACLSAKIGRQCGNCLDSYVESMGLTIEEEYFPLVNLVTGERNELEEIDGD
                     NFYIGEDNVLDLSDVVRQYASLHSSISSKCMKYCKGLCIDCGINLNKEKCNCRNNKKN
                     TKLAALLESFRPMN"
     gene            4791..4982
                     /gene="rpmF"
                     /locus_tag="QF838_08290"
     CDS             4791..4982
                     /gene="rpmF"
                     /locus_tag="QF838_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HJO60715.1"
                     /translation="MPPLPKKKHSSKRKGGRNAHNAIKPASLSNCPQCHLPRMPHRAC
                     SSCGTYNGRNVTGSDQPVV"
     gene            5015..5965
                     /gene="fabD"
                     /locus_tag="QF838_08295"
     CDS             5015..5965
                     /gene="fabD"
                     /locus_tag="QF838_08295"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HJO60716.1"
                     /translation="MTLPFARNSKTAFLFPGQGAQSVGMGLELYEGSDSGREVFEMVD
                     EALGRPLSKIMFEGPEEYLRETKNAQPGIMAVSLACMVAMKENISQESMPVPYLMAGH
                     SLGEYTALAASGVLSVMDTALLVQERGELMQDACDSNPGTMAAVLGLEEMVLQEIARE
                     SGVYISNINTDEQIVISGEKIAVVRAMDMANARGAKKVIPLKVGGAFHSGLMEPAKAG
                     LMEFIDGLKFNDPEVPIVANCTGKPLQTGEEIKGELITQISGCVQWKKSVDFMIKSGI
                     TNFLEIGPGRALGGMVKKIDRAANVSSINDIDSIVAISRN"
     gene            5982..6413
                     /gene="nusB"
                     /locus_tag="QF838_08300"
     CDS             5982..6413
                     /gene="nusB"
                     /locus_tag="QF838_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HJO60717.1"
                     /translation="MDYNPSISSRREARVLVLQALCEYDSVGHDATEVLERGFNEYKS
                     ENNSHIFAGDIVANVLENFASMDTIISELAPEWPLMQIAIVDRNILRMAIAELVWCKD
                     KVPEKVVLNEAVELAKRFGSESSPSFVNGVLGSYMKVGIAV"
     gene            6443..6697
                     /gene="acpP"
                     /locus_tag="QF838_08305"
     CDS             6443..6697
                     /gene="acpP"
                     /locus_tag="QF838_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HJO60718.1"
                     /translation="MSLLEKVQAVVAEKLSVDEAEVLPEASFTDDLNADSLDLVELIM
                     AFEEEFSSDDVEIEISDEDAESITTVQAAIDYLRDNGVED"
     gene            6857..7873
                     /locus_tag="QF838_08310"
     CDS             6857..7873
                     /locus_tag="QF838_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO60719.1"
                     /translation="MRAAVYKGKQELVIEDLPVPEPGPGEILVKVSHSAICGTDVHAF
                     LYDIAPPGAVMGHEFSGTVAAVGPGVDKWKEGDRVIGGGGTPPPGSEAPLRRQEQYNY
                     RLEGFSDTRKRGYAEFTLLNDWAPLAIPDNVSDLKAALTEPCSVAVRAVRLSNMKLGD
                     VVAVLGAGPIGLLTMQAARAAGARKIIVSEPSATRRETALRLGADAVVDPSCEDAISS
                     IINLADGNGPHVVFECAAAPSTLDAALNMVRKKGSVMLVALAWESVPMLPVDWAGKEI
                     QLNTTFGAEPYDWRVALDLISNGKVDLEPMLLDTDLLTLDGIQEAFEALIKPSTQVQM
                     VVRF"
     gene            complement(7881..8279)
                     /gene="ruvX"
                     /locus_tag="QF838_08315"
     CDS             complement(7881..8279)
                     /gene="ruvX"
                     /locus_tag="QF838_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HJO60720.1"
                     /translation="MALDVGNVRIGVAASDPTQTISTPLNSLPRKNSISEIKEICSDR
                     AIVLILVGMPYLPSGKLGTQARITEAFINELKISIEIPIQTADERMTTIEAKIRLKES
                     GYKNISKAGNKGIIDSAAAAVLLDEYISSL"
     gene            complement(8301..10955)
                     /gene="alaS"
                     /locus_tag="QF838_08320"
     CDS             complement(8301..10955)
                     /gene="alaS"
                     /locus_tag="QF838_08320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HJO60721.1"
                     /translation="MTSDEIRESFLEYFESKGHLRVPSSSLIPVGDPTLLLTIAGMNQ
                     FKPYFSGQQTPPRQRLTSSQKCFRTPDIDIVGDATHNTLFEMLGNFSIGDYFKKEAIE
                     YALEFLTDKLNLPEERFFITIHHTDDEAQEMWENIGVPKERIYRFGDDDNWWGPPIQG
                     SEGPCGPCSELHYDFGSDRGCLQTDCAPNCENIMSTKEKCTRFVELWNLVFMQYYHHP
                     DGTRENLPAPCIDTGMGLERATIVMQDAETMYETDLFSSLITQVCSISGHEYGKDLET
                     DYAIRAIAEHTRSSTFLTADGVVPGNEGRGYVLRRVIRRAIRLARKLGLQESFMPSIS
                     ESVIKKMGVTYPELHTHKEFILSVLTLEEERFQQAFENGFTMLEEAMEDSSTLEGNLV
                     FRLWDTYGFPVEMTEEIATEKNITVDMAGFQKEMEAQKDRARSHAQFDGDHARVRLYE
                     ELGVGNTNFTGYETLDGKSVVVGLISMGLSVTEVPQGQELQLVLQETPFYAEGGGQVG
                     DGGTVGNSECEIVIHDTKEAIPGLIVHYGILSKGTISIGDTVQSHVDPIRRDDTARNH
                     TATHMLHAALRHVLGPHVRQAGSLVTGSRLRFDFSHIKAVTPEEMWQVQFLVNEKIRH
                     NAEINRSEDTYRGAIEKGALAFFGDKYGETVRLVEIANGDTFSFEVCGGTHVNQTGEV
                     GAVYVLGESSIGAGMRRIEAISGREAERLVWQNMQRDSRIADILQTTPSDMENRIMAL
                     SDQISSANNQIQKLERQMSALAASALLDEVQIVNDIKVLTSITEDPSAELLRSTGDWL
                     RDKLGTGVVAIGSIVNENPMIVCMVTPDLVEKGMNAVNIVREVAKIIGGGGGGRPESA
                     QAGGKDSSKLKEALSIVPSIISDLSEGR"
     gene            complement(11078..>11300)
                     /locus_tag="QF838_08325"
     CDS             complement(11078..>11300)
                     /locus_tag="QF838_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HJO60722.1"
                     /translation="EWQHDWFSNSLNDIDAKHNPKGPNFGTSKTIKGGSYLCHESYCN
                     RYRVAARSANTPDSSTGNLGFRCVSDIKS"
BASE COUNT         2977 a         2448 c         2436 g         3439 t
ORIGIN      
        1 gtgctccgag actttttgcg gtgtcggaat atataccacc caattcgtta ctcttccagt
       61 tctcagtagc gtgaggaaag tgatcgtaat agtgggtcat aaccccatta ttcaatacaa
      121 ccgttattgt cccaagttca gatctgctag cggtctctat atccaaacct gacataccaa
      181 acgcagcgtc tcccatgaca ttgatgacta ttttatcggg cgcagcaact ttggcaccaa
      241 gtgctaatcc tagaccgtat cctagctgtg ttgacttgcc ccaacctata tatgagcgag
      301 gggtgagagc tggccaaaat ggaacaagct gatttctagg atacccagag tcatgagtga
      361 cgatagcatt tctaggatcc acagccttca tcatctcatt gaatactcga tacgggctca
      421 taggggtttc atctgaattt aatcttggtc cccattcttt catgaattca tcttttattt
      481 tgactatgtt ttcttttacc ccgtgtacat cacccctacc atgctcacct agctgagatt
      541 ttgcctcctc aatcagttgc tgcaaaacca atttagcatc accgactgcg ccgtattcga
      601 cacgatagtc tctgttaatg tcctctgcgg tgttggtaat ttgtgcttta gtggcttctg
      661 gcggcattgg tgcagtgaag tttgatattg cgaaactagt tcctattccc aaaacgaaat
      721 ctgtgttttg gaggaacctg tcgaccatta aggtgcctgt attgcctccg gtgcccaatg
      781 ctagagagtg atcttctggg taggcacttt taccagcgag ggtggtcatg actggtacat
      841 tgactatctc agcaaattct attagctcgt ctgtggcttc tgcgtataat gtgccttggc
      901 ccgcgtttat aacaggtgct tttgccttaa gcaaagcggt gactatatct cttacatcct
      961 catttgctgc atacgattta tatgagcgac tgggttggta ttcttccata tctgccggaa
     1021 cttcagcatg agcaacgtca cccggcaatt caagtaatac aggaccaggt cttccatttc
     1081 gcagatgagt gaatgcttgg ctaaccattt ctggtattcg ttcgatcgtg tttatacgac
     1141 ctgcccattt agttatgtgt tggtaatttg ggattgcgtc aaatccggga tggactccca
     1201 cccttcttgc agtagatcct cccggtatta gcaacatggg tacattatca gcataagctt
     1261 gtgcaacgcc tccaaatgca ttttctgcac ctgggccgtg ttgcatggta aagaccccga
     1321 tcttgttacc attgtggatc cggctgtatg cgtcagccat atttaccccc gctcgttctt
     1381 gtctgcagag aatgggtctg attccctcct gtgagcaagc ctcaatcaaa gtttgggccg
     1441 gaaagcagga aatccattcg gttccttctt ttttcagtaa gctggccatt aattggtcac
     1501 cgttcatgta acatctccta aattatttct gctttttgat tattgtctaa taaatttttg
     1561 tattcgtctt ccttccaaaa cctctccaac atagatatcc tttgaagagt ccacagcaat
     1621 accgtgagga gcgatgaaat gacctgcgga agggctactt tcatcagccc accggctgat
     1681 caagttccct tctgcatcta atattgaaat tctgtgttgc aactctgata cgtatgcttc
     1741 tccatctggt ccgaaggcta tatcggttgg ttgtaagaat ccatcccata taaacatgaa
     1801 ttccccatct tgatcggtaa attggattct atggttttcg cggtcacaaa ttgctagtag
     1861 cccatctttg ttgaggccta ttccatgggg taaatggaaa tccattggat tatcttttcc
     1921 tgcttcaccc caagtatcta gtaatgcccc atctggtgag aacttatgga atctacaatt
     1981 tccgtaacca tcagacacga aaatattacc gtcatcagca acaactaccc ccgtcggcaa
     2041 attaaaaggt cccgccgggt gcggaactac cattagattt ccgctgtagc cagtatttga
     2101 ttcaacattg taggtgccga gagtgagtag aagtgtttgg tctggactat atttaaggac
     2161 aacatgggcg tcacggtcag caaaataaaa atttccatct ggtcctatat gcattccatg
     2221 ggggttggaa aatttaacgt cccatgattt caaaaaatcc ccatttttct taaatatcat
     2281 gacctggtgt tcacttcggt tatagaggta gacattatct tccctatcta cggctacccc
     2341 tgccacctga ttgaattcgt acccctctgg caacctgccc caatcttgat cgacttcgta
     2401 tgtatagtcc ccactaccgt atggcattat gagattctcc tctatattga ctaatctaga
     2461 tcaatctatg attttgttat cgtagcaaga acccatactt taggtttata tgtgggattg
     2521 gtcaagaaca gtctaaatta aggcttcctt tgctgcattt tccccggcta ttttcccaaa
     2581 tactgcaccg gacattaaac cggatcctcc cgggtagttg ttgtaaaaca accctcctgt
     2641 gagttccccc gcggcgtaaa gcccaggtat gggcatatca gaattatctt ggacttgtgt
     2701 gttcgtatct atttttagac caccaaatgt aaatgttatt ccacaggtca cggcataccc
     2761 caaataagga ggtttatcta atttttgcgc ccagttggac tttggagggg taatcccttc
     2821 agtgtgcttc ccgtcaaggg cagtgggatt aaaatcacct tcttgtactg ctccattgaa
     2881 ttcttgaaca gttatttgca gctgcccggg attaatatct aatccggtgg ccaattcttc
     2941 gattgtcttg gcttccacca tcgtcatttg gggaataaaa tattcatctc ttagtaaatg
     3001 tttagtcttc tgatcaaaga gctgaaaggc aaccctttga ggttggttca atatttcgcg
     3061 gccgtatttt gcatatgtgt agtttctcaa atcggcacct tcatcgacaa atctttctcc
     3121 atcaacattg actatcaacc caaaggggta agagtgcttt tggaataggt ctgcgacatt
     3181 tctatcaccg tgaggcggtg catttagatc ccatgcaacc gcatggcacc cactccagtg
     3241 cccatgcgat tgtgccccaa ttcggagagc catatttatg ccatctccgg tgttatattg
     3301 ggttcctcta accttggcga gttcccaacc tggtcctaaa tacctggagc gcatctctgt
     3361 gttcgcctca aagcctccac aggcaagcac aacggattta caggaaattt tctcatatcc
     3421 agattttgat ttcgcaatga ttccttcaat atgtccttct tgattgattg acaaatctgt
     3481 accactagtt tgatacagaa cttcaatatt ctctcgttca actaattcaa aaagcctatc
     3541 tgacaatcct ttgcctccac cgaccgcttc gacaatcaac cctccccaga atctttgttt
     3601 accttcatgc tcaaaagctt gtctgccgta agcaagaatc catggaactc cttgatttcg
     3661 catccaaagc atggttggga atgattgaga ggttagtact tgaagtaagg aagggtctga
     3721 taagccttca gtaactctca tgacatcgga ataaaactca gattctttgt aagaccctac
     3781 atcaatgttt tgtttttctt ttgatgttag gtctggtaat aattctgaaa tatcttctat
     3841 tccgttgtat gcaaatcgga aaagcccacc tgtgaaatag gaattacccc ctcgtaggtg
     3901 ttcaggagct ttttcaagga tcgccacttt cacacctttt gatgcagcgg acagtgctgc
     3961 agttaaggcg gcatttccgg cccccactat cactacgtca tagttttttt cgggagatgt
     4021 cattgttttg cctttttcat tgggttgttg atggggtcaa ttggatattt ttgtttcccc
     4081 agccaatgta atctaacctt accggatttt caaactctta tattctaaat gaatcaggtg
     4141 tgaaataatt gattgaaaat agccttcatt ccggttgacc gggttccctt accaaagata
     4201 tagtgtttgc ttgaattgtt gctttgaatg gatatcctga ggcgtgctat ggactttaaa
     4261 gtagctacct tgctcaaggc gagtagtggt cataaaaagt tggaaagtgt aagcagtacc
     4321 atcgaagtgg attggtctga atttagatgc gatgttgatg gttcagtttt attgaccaag
     4381 actgatgtgg gagtatgggt gagtgcttgt ctttcggcta aaataggtag gcaatgcgga
     4441 aattgtttag attcatatgt tgaatctatg ggcttgacga tagaggaaga atacttccca
     4501 ttggttaatt tagttactgg tgagagaaat gaattagaag aaatagatgg ggataatttt
     4561 tatatcggag aggataatgt actagattta tccgatgtgg tgcggcaata tgcttctttg
     4621 cactcatcta tcagttcgaa atgcatgaaa tattgcaagg ggttgtgcat agattgcggt
     4681 ataaatctta ataaagaaaa atgtaactgt agaaataata aaaagaatac taaattggct
     4741 gctttattag agtcttttag accaatgaat taagaaataa gggaattaac atgccaccac
     4801 tacctaagaa aaaacactcc agcaaaagga aaggcggtcg gaatgcacat aacgctataa
     4861 aacctgcctc actttctaat tgcccccaat gccatctccc tcggatgccg cacagggctt
     4921 gttcgagctg tggcacatat aatggccgta acgtgaccgg ctctgatcaa ccagttgtat
     4981 aaatctcgac cgtaacttac taaaggacat taatatgaca cttccctttg caagaaattc
     5041 caagacagct tttctctttc ctggtcaagg tgcccagtct gtaggaatgg ggttggaact
     5101 ctacgaaggt tcggattcag gcagggaagt gtttgagatg gtcgatgaag ctttaggtag
     5161 acctctatct aaaatcatgt ttgaaggtcc agaagagtat ttgcgggaaa ccaaaaatgc
     5221 acaacctgga ataatggcag tgagtttggc ctgtatggta gcgatgaagg aaaatatttc
     5281 acaagaatcc atgcctgttc catatctaat ggcagggcat agccttggcg agtacacagc
     5341 cttagcggct tcaggggtcc tgtcagttat ggacacggct ttactagtac aagaacgtgg
     5401 ggaacttatg caagatgctt gcgattcaaa cccaggtacc atggccgcag ttttggggtt
     5461 ggaagaaatg gttctacaag aaattgcgag ggaatctggg gtatatatat caaatataaa
     5521 caccgatgaa cagatagtga taagtggaga aaaaatcgca gtagttagag ctatggatat
     5581 ggcgaatgct agaggagcga aaaaagtaat ccctttgaaa gttggagggg cttttcattc
     5641 tggattaatg gagccggcaa aggctggtct catggaattc atagatgggt tgaaattcaa
     5701 tgaccctgaa gttcctattg tggccaactg cacaggtaag cctctccaaa caggtgagga
     5761 aataaaggga gaattgataa ctcaaatctc aggatgcgtg caatggaaaa aatctgttga
     5821 tttcatgata aagtcaggga taacaaattt tctcgagata ggtccggggc gagctttagg
     5881 gggaatggta aaaaagattg atagagctgc gaatgtatcc agcatcaatg atatagattc
     5941 tattgtagca attagcagga attagcgaag agtccctata aatggattat aaccccagta
     6001 tctcaagtag aagagaagcc agagtgttgg tattacaagc cttgtgtgaa tatgactctg
     6061 tgggtcatga cgcaacagag gtccttgaga ggggtttcaa cgaatataaa tccgagaaca
     6121 actctcacat atttgctggt gacatagttg caaatgtact tgaaaacttt gcctcaatgg
     6181 ataccataat tagtgagtta gctccagaat ggcctttgat gcaaatagca atagtggacc
     6241 gtaacattct tcggatggca atcgcagaat tagtatggtg taaagacaag gttcctgaaa
     6301 aggtggttct taacgaagcc gtggagctgg cgaaaagatt tgggtcagag agttctccta
     6361 gctttgttaa tggggttctt ggatcctata tgaaggttgg tatcgctgtt taggacgtaa
     6421 caaataaaaa tggaggcatt aaatgtcgct acttgaaaaa gttcaagctg ttgtagctga
     6481 aaaattaagc gtggatgagg ctgaagtttt accggaagct tcgtttacag atgatttaaa
     6541 tgcagattca ttggacttgg ttgaattgat aatggctttt gaggaagaat ttagttctga
     6601 tgatgttgaa atagaaatat ctgatgaaga cgccgaatcg ataaccacag ttcaggcagc
     6661 cattgattac ctgcgggata atggtgtaga ggactaggtc actctcacga tctatctgca
     6721 ctcataaata ggcgagaagt tccctaggat taggatactg ctggccttgt taatatccct
     6781 ctttaaactt tccttatatt gacttaggtc tttataatgc gctcatttac tatcattgat
     6841 aattaggggt gattccatga gggcagctgt atacaaaggg aaacaagaac ttgttatcga
     6901 agacttaccc gttcctgagc ctggaccagg agaaattctg gtaaaagtta gccatagtgc
     6961 catttgtgga actgatgtcc atgcttttct ttatgatatt gctccacctg gcgctgtgat
     7021 gggtcatgaa tttagcggta ctgttgccgc tgtgggcccc ggagttgata agtggaagga
     7081 aggtgataga gtcattggag gtggagggac acctccacca ggatcagaag ccccgctgag
     7141 aaggcaagaa caatacaact atcgattaga aggattttcc gacacccgga aaaggggata
     7201 tgctgaattc actttgttaa atgattgggc tcctttagcg attcccgaca atgttagtga
     7261 tttaaaggcg gcattaactg aaccttgttc tgttgcagtc agggcggtca gactgtcaaa
     7321 tatgaaatta ggtgacgtag tagctgtctt gggtgcgggt cccattggat tactaactat
     7381 gcaagctgcg agagcagctg gagcccgaaa aataatagtt tcagaaccct cagccacccg
     7441 tcgggagact gctttacgat tgggtgctga tgctgttgtt gacccatctt gtgaggatgc
     7501 aatctcctca ataattaact tggcagacgg aaatggccct cacgttgtat ttgaatgtgc
     7561 ggctgcccct tctaccttgg atgcggctct taatatggtt cggaaaaaag ggagtgttat
     7621 gctcgttgct ttagcttggg aaagtgtgcc aatgctccca gttgattggg caggaaaaga
     7681 gattcaatta aacactactt ttggcgcaga accttatgac tggagagtag ctctcgattt
     7741 gatatctaat ggcaaagttg acctggaacc aatgctgctg gacacggacc ttctaactct
     7801 agatggtata caggaggctt ttgaagcgct aataaaacca tctactcagg tgcaaatggt
     7861 tgtaagattt taagttattc ttagagggaa ctaatgtatt cgtccagtaa taccgcggcg
     7921 gcggccgagt caataattcc tttgtttcct gccttgctga tgtttttata accagactct
     7981 ttaagccgta tttttgcttc gatagtcgtc attctttcat ctgccgtttg aatgggtatc
     8041 tcaatggata ttttcaactc attaataaat gcctccgtta ttcgcgcctg ggtacccaat
     8101 tttccagaag gtaaataagg cataccgact aagattaaga caatagctct atctgaacat
     8161 atttctttaa tttcggatat tgaatttttc cggggcagac tatttagtgg agtcgaaatc
     8221 gtctgtgtgg gatcgctggc agctacccct atacgaacat tacccacatc tagagccaaa
     8281 atacttccag cgttcagagg ttaccttcct tcagacaggt ctgaaattat actgggaaca
     8341 atggacaaag cttcctttaa tttactcgaa tcttttccgc cggcttgggc cgattctggt
     8401 cttccaccgc cacccccgcc gattattttg gccacttccc gcactatgtt tactgcgttc
     8461 attccttttt cgactaagtc gggagttacc atgcaaacta tcattgggtt ttcgtttact
     8521 atagaaccta tagcaacgac acctgttcct aacttgtctc gcagccaatc tccagttgat
     8581 cgtaataatt ctgcggatgg atcctctgta atcgatgtta aaaccttgat gtcgttgact
     8641 atttgtactt catctaacaa agctgaggca gctaaggccg acatttgcct ttccaatttt
     8701 tggatttgat tattagccga agatatctgg tctgagagag ccattatcct gttttccata
     8761 tctgacggag tggtttgcag tatgtccgcg attctagaat cacgttgcat gttttgccat
     8821 acaagtctct ctgcttctct gccactgata gcttctattc ttctcattcc tgcgccaata
     8881 ctcgattcac ccaaaacata tacagctccg acttcaccgg tttggttcac gtgcgttcct
     8941 ccacatactt caaaactaaa agtatccccg tttgcgattt ctaccaaccg cactgtttct
     9001 ccatatttat ccccaaaaaa agctaaagca cccttttcaa ttgcccccct ataggtatcc
     9061 tcactcctat taatctctgc gttatgcctt attttttcgt tgaccaggaa ttgtacctgc
     9121 cacatctcct ctggagtaac agcctttata tggctgaaat caaaacggag tctggaccca
     9181 gtcaccagag acccagcctg tcttacatgg ggcccaagca catgcctcaa cgcggcgtgc
     9241 aacatatggg tagcagtatg attcctggcg gtgtcgtctc ttcttattgg gtcgacgtgg
     9301 ctctgtactg tgtccccgat tgaaatggtt ccttttgata aaattccgta atgcacaatg
     9361 agcccgggga ttgcctcttt tgtgtcgtga atcacaattt cacattcaga gttaccaacg
     9421 gtgccgccat cccctacctg ccctccaccc tcggcataaa aaggagtttc ctgtaaaacc
     9481 aactgtagtt cttgaccttg ggggacttca gtaacagaca gacccattga aatcaatcca
     9541 actacaacgc ttttaccgtc cagtgtttcg tatccggtga aattagtatt cccgacccct
     9601 aattcttcgt ataaacggac tcgcgcgtga tcaccatcaa attgtgcgtg agatcgggcc
     9661 cgatctttct gggcttccat ctctttttgg aaaccagcca tatccactgt aatatttttc
     9721 tcggttgcta tttcctcagt catttccact gggaatccgt aagtgtccca gagcctaaaa
     9781 actaggtttc cttccaaagt agaagaatct tccattgctt cctctaacat ggtaaatcca
     9841 ttctcgaaag cctgttggaa tctttcctcc tctaatgtca agacacttag aataaattcc
     9901 ttatgggtgt gtagttcagg gtaggttact cccatttttt taatgacaga ttcagaaatg
     9961 gatggcatga aggattcctg caatccaagc tttcgggcaa gcctgattgc tcttcgaata
    10021 actcgcctta gtacataacc tctgccttcg ttacctggga ctactccatc agctgtcaag
    10081 aatgtggaac ttctcgtatg ttctgcaatt gccctaatcg catagtcagt ttccaaatct
    10141 tttccgtatt cgtgcccgct gatagaacaa acttgagtta taagagaaga aaatagatct
    10201 gtctcgtaca ttgtttctgc atcttgcatc acaatggtag ctctttccaa tcccattcca
    10261 gtgtcaatac acggggcagg taaattttct cttgttccgt cagggtgatg gtagtattgc
    10321 atgaatacca agttccatag ttccacgaac cgagtacatt tctccttagt ggacattata
    10381 ttttcacagt taggggcgca gtcggtttgg aggcagcctc tatctgatcc gaagtcataa
    10441 tgtaattcac tacatggccc gcaaggccct tcacttcctt gtatgggagg gccccaccaa
    10501 ttgtcatcat caccgaatcg gtatatacgt tccttgggca ctccgatgtt ttcccacatt
    10561 tcctgagctt catcatctgt gtgatggata gtaatgaaaa atctttcctc tggaagattt
    10621 aatttatcag tcagaaattc caaggcatat tcaatggctt cttttttaaa atagtcccct
    10681 atactaaagt tacctaacat ttcaaataat gtattgtgag tagcatctcc gacaatatct
    10741 atatctggag ttctgaagca tttctgagaa gaggtcaatc tttgtcttgg aggagtttgt
    10801 tggccagaaa aatatggttt aaattgattc attccggcaa ttgttagtaa aagggtaggg
    10861 tcaccgacgg gtattaaaga tgagcttggt actctgagat gtcctttact ctcaaagtat
    10921 tctagaaagg attctcttat ttcatcacta gtcattttat tggtcatcca actactcgtt
    10981 tgcataatgg gcactaatac tctttggtga agtcatcagg atctggctta actggcaaaa
    11041 ttatatcgca gtgtttttaa tgtcgctttc gacgatatta ggatttgata tctgaaacac
    11101 atctaaatcc cagattcccg gtggaactat ctggtgtatt tgcacttcta gcggccaccc
    11161 gatatcgatt acaataggat tcgtgacata agtacgaccc cccttttatg gtttttgatg
    11221 tgccgaaatt agggccctta gggttatgtt tggcatctat atcgttgagt gagttactga
    11281 accaatcgtg ctgccattcc
//
LOCUS       DASCNS010000228         1979 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001779354, whole genome shotgun sequence.
ACCESSION   DASCNS010000228 DASCNS010000000
VERSION     DASCNS010000228.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001779354"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(260..712)
                     /locus_tag="QF838_08330"
     CDS             complement(260..712)
                     /locus_tag="QF838_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60723.1"
                     /translation="MASNPEEALRDSVEALGTTSKMYRETLHLPMHHISVGVTPIAFY
                     LEEENVPNYFADFYSEIRPEWIGSTCTLESYSEVIKDDQKIAYLLKASRLDKGGSIMS
                     TFEGIFTLGKIGQDWRLISRNIFNVSKESKLRQRELIDKWNEKSQELR"
     gene            899..1735
                     /locus_tag="QF838_08335"
     CDS             899..1735
                     /locus_tag="QF838_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60724.1"
                     /translation="MSRLKNIRLVLAGAFIVPVALASIACSPIYADDPNGVQREIIAP
                     DLDLEALNARIQSAVESGEISQAQVATAIEILKSEFSVTPKTRFKPFMGIEKIKAEIL
                     AAVESGEITQEEADAKLAGLEERKERGQMPHGKRHHHKKPFMGIQDIRAKISAAVESG
                     EITQEEADEKLADIEAKRNAGQEKMDAKLESIKAEILAAVESGEITQEEADAKLAGLE
                     ERKEKGQKWHGKRHHHKKPFMGIEEIKAKILAAVESGEITQGQGQDKLRYFKDGPKKV
                     IY"
     gene            1750..>1979
                     /locus_tag="QF838_08340"
     CDS             1750..>1979
                     /locus_tag="QF838_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HJO60725.1"
                     /translation="MNSSVLIADDDIAICEALERSLRFEGFKVRTALNGELAIVEIEK
                     DIPDILILDINMPEVDGIQVTKYLRSLDIDIP"
BASE COUNT          645 a          347 c          441 g          546 t
ORIGIN      
        1 ggaaaattcc aatttagcga taaaattgaa actatcagga aatatattcc caagcagcac
       61 acctgttgct atgcaaaatg cgacccaaat tgagagataa cgctcaaaga atcccataaa
      121 gatcaccttt tgccagtaag cgaatccggc tatagctcat caagttgcat tcatttgtaa
      181 aaattaacat tttagataca aaaaaacatt agtaaaatgg tgcaaactat ggtttatgta
      241 aattagtctc gataacagac tatcttagtt cctggctttt ttcgttccat ttatctatca
      301 attctcgttg cctgagtttg gattccttag aaacattaaa aatattccga ctaattaatc
      361 gccagtcttg ccctattttc cctagtgtaa aaataccttc gaatgtggac attattgaac
      421 cacccttgtc taagcgtgag gctttaagga gataggcaat tttttggtca tcttttatga
      481 cctcagaata ggattccaat gtacaggtac tccctatcca ctcaggacgt atttctgaat
      541 agaagtcagc aaaatagtta ggaacattct cctcttctag gtaaaaggct atgggagtta
      601 ctccaaccga aatgtggtgc ataggtaagt gaagagtttc cctatacatt ttggatgtgg
      661 tccctaaagc ctcgactgaa tctcttaatg cttcttctgg atttgatgcc attttgtcaa
      721 ttactcccaa aatgtatttg ttttactaga aagggtatta ctaatcctaa caatgattaa
      781 cagacatatg gaattataaa ttcttattaa attcttagaa tatcctcaaa gttctttaag
      841 gaaactttac tagtctttct ctatcggttt attagataat acaaaatgga gaaagaacat
      901 gagtcgacta aagaatataa ggttggtatt ggcaggggcg tttattgtcc ccgtggcact
      961 cgcttcaata gcttgttccc cgatttatgc tgacgaccca aacggggtac agagggaaat
     1021 aattgcccct gacctggatc ttgaagcctt gaatgcaaga attcaatcag cggttgaaag
     1081 tggggaaatt tctcaagctc aagtggctac tgctatagaa atacttaaaa gcgaattttc
     1141 ggtaacccct aaaaccagat tcaagccttt tatgggtatc gagaaaataa aggctgaaat
     1201 attggctgct gtagagagtg gtgagataac tcaagaggag gctgacgcca agctggctgg
     1261 gttagaggaa cgaaaagaga ggggtcaaat gccacatggg aaaaggcatc atcacaaaaa
     1321 gccttttatg ggtatccaag atattagagc caagatttcg gctgctgtag agagtggtga
     1381 gataactcaa gaggaagctg atgaaaaatt agctgatatt gaggccaaga ggaatgccgg
     1441 acaggaaaaa atggatgcca agctcgaatc aataaaggct gaaatattgg cagctgtaga
     1501 gagtggtgag ataactcaag aggaggctga cgccaagctg gctgggttag aggaacgaaa
     1561 agagaaaggt cagaagtggc atgggaaaag gcatcatcac aaaaagcctt ttatgggtat
     1621 cgaggagata aaggcgaaaa tattggcagc tgtagagagt ggtgagatta ctcaaggaca
     1681 gggccaagat aagcttcgat atttcaaaga tgggcctaaa aaggtaattt actaataaat
     1741 aggtgtctca tgaattcatc cgtattgata gcagatgatg acattgcaat ttgcgaggcc
     1801 cttgaaagaa gtttgcggtt cgagggcttc aaggttcgga ccgccttgaa tggtgaattg
     1861 gccattgtgg aaatagaaaa agatattcca gacattctca tactcgatat taatatgccg
     1921 gaagtggatg gtatccaggt caccaagtac cttcgaagtc tagatataga tattcccat
//
LOCUS       DASCNS010000229         5784 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001782047, whole genome shotgun sequence.
ACCESSION   DASCNS010000229 DASCNS010000000
VERSION     DASCNS010000229.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001782047"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            174..908
                     /gene="trmD"
                     /locus_tag="QF838_08345"
     CDS             174..908
                     /gene="trmD"
                     /locus_tag="QF838_08345"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HJO60726.1"
                     /translation="MMKFHIFTLFPNLFASPLEEGVIGRALKSGLVQININNIRDHSG
                     DKHGTVDDYPFGGGPGMLLKPEPIFKSVQSNIERNRIKRDSPLILLSPQGRQFNQDIA
                     KELSLHEEISLICGRYEGFDERIRCNLVTDEISIGDFVLSGGEIPAMAIIDSVSRLLP
                     GVINSSDSAKNDSFYDGLLQYPQYTRPAEYQGMKVPPILLSGNHSEIEKWRRRESLKK
                     TKLQRPDLISSLSLSEEERTFLENIQ"
     gene            999..1913
                     /gene="argF"
                     /locus_tag="QF838_08350"
     CDS             999..1913
                     /gene="argF"
                     /locus_tag="QF838_08350"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HJO60727.1"
                     /translation="MIGKDFLTIHDLEPGGVLGLVQKADLMKKSPPPQVLTNKTVALL
                     FEKPSLRTRVSFEVGVKQMGGSCIFLSNAEVGLGVREPEADVARVLDRLVDCIVARVF
                     SHNSLIELANNTDLPVINALSDKGHPCQAMGDCLTVYEHKGKLEGLKMAFIGDGNNIA
                     GSLALACSDAGMDFSISSPEQYQLPAEIWKSAQEISNTKGTNLMWTPDPKEAAKEADV
                     IYTDVWVSMGDEGEKKERLETFAPYQVNESLVEYAKPNFLFMHDMPAHRGEEISNSML
                     DHNNSVVYQQAENRLHAQKTIMSELLSG"
     gene            1971..3320
                     /gene="der"
                     /locus_tag="QF838_08355"
     CDS             1971..3320
                     /gene="der"
                     /locus_tag="QF838_08355"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HJO60728.1"
                     /translation="MQTGKEQLKKPIVAIVGRPNVGKSTLFNRLIGQRMSIVSDIAGT
                     TRDRIISETDWAGNPFILVDTGGLDLFPNDDIWDQVKSQIHYAIGECDVVILLVDISD
                     GVTSNDQDIVDILRKTGKPVVAVASKADNEIRDHMVSELYSLGMGDPVSISAYHNRGL
                     DDLMERVVAHFPADNSFPEPEADLGLAIVGRTNVGKSMLLNTLSGENRAIVSDTAGTT
                     RDSLDVVIKHEDKDIQLIDTAGIRRRGQIEYGIEKYSVLRSIQSIDRCDVAALVIDAS
                     EFATAQDTHVANYILEAHKGIVILVNKWDLSDQLATNRQEMANQVRSKFKFAQYAPIC
                     FTSGLTGSGIKNMLDTAINVQSERSKIIPRAKVRRTIMTAVANHPPPVHGKKELKIYS
                     ALQDTSSPPSFTFFVNHADMVHFSYKRYLENSLRENFGFEGSPLRLRFSGRSRKTDQ"
     gene            3317..3943
                     /gene="plsY"
                     /locus_tag="QF838_08360"
     CDS             3317..3943
                     /gene="plsY"
                     /locus_tag="QF838_08360"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HJO60729.1"
                     /translation="MIIYLEGIIFVIFSYVLGSISFGLIITKMVKGIDIRQYASGSTG
                     ATNVMRACGWRWGLLAFALDMFKAALPLLIVIYIYDFGIPNWVHPIMGLAAIFGHIWP
                     VFTNFRGGKGIASGWSALIVLSPWSGLIACVLSFPFMLFTRYVSLGSVIGSLSGGIAI
                     TTLSITGHIETSYCAFGLVGSALTILKHKDNMIRLLNGTERKVGATND"
     gene            3971..4972
                     /locus_tag="QF838_08365"
     CDS             3971..4972
                     /locus_tag="QF838_08365"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HJO60730.1"
                     /translation="MAHIAVIGPTTWGTTLAIINAEEGKHVSLLVRTEKESNLLRKHR
                     ENQRFLPGITFPDTLEITSRSQDLIPKTNLLVIAVPSQTLRENVRAISNYISEPTIIV
                     SASKGLESTSGRRMTEIIEEEIPNLSPDNLCALSGPNLSNEILAGKPSSSVISSINLT
                     TAKSAQSILKCSQLRLYTNTDVIGVEICGALKNITAIAAGICDGLNLGDNAKASIITR
                     GLAEMARFGSAIGAKSSTFSGLAGMGDLIATCSSRLSRNNQVGRMLAKDISLSQIEKS
                     MDNVAEGIETTKAVVLVSNQLNIDLPIAQAIYNILFKNLSVKDAREELMGRTLTSET"
     gene            4982..5335
                     /locus_tag="QF838_08370"
     CDS             4982..5335
                     /locus_tag="QF838_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HJO60731.1"
                     /translation="MQIIQLFTASLLTGFFGAIMPGPVLIYTIGRSLSHGFISGALAV
                     IGHSLLELFLVIGLTFGLDRLLNNQIAEFVIGTAGGLFLLWMGWGMLKTQLVARYQLS
                     INQMLIPVEDLFQRE"
     gene            complement(5629..5784)
                     /locus_tag="QF838_08375"
     CDS             complement(5629..5784)
                     /locus_tag="QF838_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HJO60732.1"
                     /translation="MIGPIGGFTPQEVSDAVDCGATSVSLGKRILRSETAGIVAAVAV
                     LYEFDDI"
BASE COUNT         1794 a         1132 c         1256 g         1602 t
ORIGIN      
        1 ttatcgtaaa tctgccgggc agcccgagag gggtggaaga atgcctagat gtgataatgg
       61 aatccttacc acatgctttg gaaatgataa gaggttggaa aacacataaa gaatccgatt
      121 aaccgattga accgattgaa ccgattgaac cgtaggaatt atttcttaaa aaaatcatga
      181 aattccacat ttttacttta ttccctaacc tgtttgcaag ccccttggag gaaggggtca
      241 ttggacgagc cttaaaatct ggtttagtcc agataaacat caataacatt agagatcatt
      301 ccggagacaa gcacggaaca gtagatgatt accctttcgg tggtggccct ggtatgttgc
      361 taaaaccaga acctattttt aagtcagtgc agtccaacat agaacggaac aggataaaac
      421 gcgattcccc tttaatttta ctgtctcccc aagggcgaca attcaatcaa gacatagcaa
      481 aggaactttc actacatgag gaaatatcct taatctgtgg acggtatgaa ggttttgatg
      541 aaagaattcg ctgcaatctg gtcacagacg agattagcat cggtgatttc gttcttagtg
      601 gcggagaaat tcctgccatg gctattatcg attcagtatc tcgcctactt cctggcgtta
      661 tcaatagttc ggattccgca aaaaatgatt cattttatga cggtttatta cagtacccac
      721 aatatacaag acctgctgag taccaaggaa tgaaagttcc tccgatcctc ttatctggaa
      781 accactctga aatagaaaaa tggcgaagaa gagagtctct aaaaaaaacc aaactccaaa
      841 ggccagatct aatatcgtca ctttccctta gtgaggaaga gagaacattc ttagaaaata
      901 ttcaataata gatataaatc cctaagtcgg atcagaattt ctgctatcgt tctaaaagtg
      961 aaaagcccca tacccgagga aaacagggag tgagtttaat gataggcaaa gattttttga
     1021 caattcatga tttggaacct ggaggcgttc ttggtttagt acaaaaagcg gatttaatga
     1081 agaaatcccc acccccacaa gtactaacga acaaaactgt agccttgcta tttgaaaaac
     1141 cttcactcag aactagagtt agctttgaag tgggagtaaa acaaatgggc gggtcatgta
     1201 tctttttaag caatgcagaa gttgggttag gtgtgagaga acctgaggca gatgtggcca
     1261 gggtcttaga cagattagtc gattgtatag ttgcccgagt tttttcacat aatagtttaa
     1321 tcgaattggc aaataacact gacttgccgg taataaatgc cctttctgac aaagggcatc
     1381 cttgccaggc aatgggagac tgtctcacag tatacgagca taaagggaaa ctggaaggtt
     1441 taaagatggc tttcataggc gacggaaaca atatagcagg aagtttggct ttagcctgtt
     1501 cagatgccgg aatggatttt tctatttctt ctccagaaca ataccaatta ccagcagaaa
     1561 tttggaagtc ggctcaagaa atttctaata ctaaaggcac caatcttatg tggactcccg
     1621 atccaaaaga ggctgcaaaa gaagctgatg tgatatacac agatgtctgg gtaagtatgg
     1681 gagatgaagg agagaaaaaa gaaaggctag aaacctttgc cccataccaa gtaaatgaat
     1741 ctttagtgga atacgctaag ccaaactttc tattcatgca tgacatgcca gcccacagag
     1801 gagaggaaat ttcaaatagc atgcttgacc ataacaattc tgttgtgtac caacaagctg
     1861 aaaatcggct tcatgcccaa aaaaccataa tgtctgaatt actttcaggc tgacctctca
     1921 gtatttattt tcaaatagca atataaatat acaaatttag gctaaacgat atgcaaacgg
     1981 gaaaagagca actcaagaaa ccaatagttg cgattgttgg acgacctaat gttgggaaat
     2041 caactctatt caatcggctt atcggacaaa ggatgtcaat agtctcggac attgcaggta
     2101 caacccgaga cagaataata tcggagacag actgggccgg gaatcctttc atattggtgg
     2161 acacaggagg gttagacctg tttccaaatg acgatatttg ggatcaagta aaatcacaaa
     2221 ttcattacgc catcggtgaa tgtgatgtgg tgattctatt agtagacata tcagatggag
     2281 ttacatcaaa cgatcaagat attgtcgata tactccgtaa gaccggcaaa ccagttgtgg
     2341 ctgtggccag taaggcagat aacgaaatcc gtgatcatat ggtctcagaa ttatattccc
     2401 tcggtatggg tgaccctgtg tcaataagtg cctaccacaa caggggcttg gatgatttga
     2461 tggaaagggt agtggctcat tttccagccg ataactcctt tccagaaccc gaagcagacc
     2521 ttggtttggc gattgttggt cgtacaaacg ttgggaaatc aatgttgcta aatactttgt
     2581 ccggagagaa tagggctatt gtcagcgata cggctggcac aactcgagat agcctagatg
     2641 tagtcataaa acacgaggat aaggatattc agttaataga tacagctggc atacgtcgga
     2701 ggggccaaat tgaatatgga attgagaagt atagtgtgct tcgttcgatt caatccatcg
     2761 atcgctgtga tgtagcggcc cttgttattg acgcttcaga gtttgcaact gcccaagaca
     2821 cgcatgtagc caattatatt cttgaagcac acaaaggaat cgtgattcta gttaataagt
     2881 gggatctatc agatcaactt gccacaaaca gacaggaaat ggccaatcag gtgagaagca
     2941 aatttaaatt cgcccaatat gctcctattt gctttacttc aggattgaca gggtcgggaa
     3001 taaaaaacat gttggatact gcaataaacg tccaatctga aaggtccaaa attattcctc
     3061 gcgcaaaagt tagacgaacg attatgactg cagttgccaa tcacccacca ccagtacacg
     3121 ggaaaaagga actgaaaata tacagtgctt tacaagatac ttctagccca cccagcttca
     3181 ctttttttgt gaatcatgca gacatggttc atttttccta taagcgttat ttggaaaata
     3241 gtttgagaga aaatttcggg tttgaaggga gcccactaag acttcggttt agcggtagaa
     3301 gtaggaaaac tgaccaatga tcatctactt agagggcata atttttgtta ttttcagtta
     3361 tgtattaggt tccatatcct ttggcctgat tatcacaaaa atggtcaaag gaattgacat
     3421 taggcaatac gccagtggca gcaccggggc cacaaatgtt atgcgcgcct gtgggtggcg
     3481 ttggggccta ttggccttcg cattggatat gttcaaagcc gccttacctc tgttgatagt
     3541 gatctatata tatgattttg gtattcctaa ttgggtgcat ccaataatgg ggctagccgc
     3601 gatttttgga catatatggc cggtatttac aaatttccgc gggggtaagg gaatagcctc
     3661 tggttggtcg gctctaattg ttctgtctcc ttggtcaggg ttgatagctt gtgtcttatc
     3721 attcccattt atgttattta cgagatatgt atctctggga tctgtaattg gttctctatc
     3781 tgggggtatc gcaataacta ccctgtctat cactggacat attgaaacat cgtattgtgc
     3841 attcgggttg gtaggatctg cgttgaccat attgaagcat aaagacaata tgattcggct
     3901 attaaacggt actgagcgaa aagttggagc aacaaatgac taaattatta atgtcttacc
     3961 tcgtagccat atggcccata tagcagtcat tggcccaact acatggggca ctacactggc
     4021 cataattaat gcagaagaag gaaaacatgt ctctctgcta gtaagaactg aaaaagaatc
     4081 aaatctacta cgaaaacatc gtgaaaacca acgctttcta ccaggaatca cattcccaga
     4141 tacccttgaa ataacttcaa gaagccaaga tcttattccg aaaacaaatt tgcttgtgat
     4201 agcagtacca tcacagacat tgagggaaaa tgtcagggcc atatcaaact acatttctga
     4261 acccaccatc atagtaagtg cctcaaaagg tctggaatct acttctggca ggagaatgac
     4321 agaaataata gaagaagaga ttcccaattt gtcacccgat aatctatgcg ccctctctgg
     4381 acccaaccta tctaacgaaa tcttggctgg caaaccttct tcatcagtaa tatcttccat
     4441 taatttaaca acagcaaaat cagctcaatc gatacttaaa tgttctcaac tacgtttgta
     4501 cacaaacact gacgtaatag gggtagaaat atgcggggcc ttgaaaaata taacagcgat
     4561 cgccgccggt atatgtgacg gacttaactt aggagataac gcaaaggctt cgatcataac
     4621 tagaggtctt gcagagatgg cccgtttcgg cagtgccatc ggtgctaaat catccacttt
     4681 ttctggcttg gctggaatgg gagatctcat agcaacttgt tcttcaagat taagtcgcaa
     4741 taaccaagtt ggcagaatgc tagccaaaga tatatcttta agtcaaattg aaaaatctat
     4801 ggacaacgtg gctgagggca tagaaacgac aaaagctgtt gtattagtgt ctaatcagtt
     4861 aaatatcgac ttaccaattg cccaagctat atataatatt ttgtttaaga atttgtcggt
     4921 gaaggatgct agagaagagt tgatgggtag aacattaact tcagaaacct aggttggata
     4981 cttgcagata atacaactat ttacagcttc tctattaaca gggttcttcg gcgccataat
     5041 gccaggaccg gttcttattt acacaattgg tagaagtcta tctcatggat ttatttcagg
     5101 agctttggcg gtgataggcc actcactgct agagttgttt ttggtaattg gcctcacttt
     5161 cgggttagac cgactcctta acaatcaaat tgcagaattc gttatcggga cggcgggtgg
     5221 cttgtttcta ctttggatgg gatggggaat gttaaaaacc cagttggtag cgcgatacca
     5281 attgtcgata aatcagatgc tgattccagt cgaggaccta tttcagcggg aataatttta
     5341 agtgcaccta acccatattt ttcgtttggt gggctacagt tggccttagt ttcattgaag
     5401 aatcaagaga agccggaacc gctggtatag ctgcctttta tacaggtcac atgtctgccg
     5461 acatagtcta gtatgcgatg attgctttaa gggtttcttc aggcaaaaag ttcctgagtg
     5521 atacaattta taaagtaata gtgcgttgct gtggcatttt tctaatatgt ctaggtctat
     5581 atttcgccta cgccggcaag gattttatgc aacaaatatt gacctaattt aaatatcgtc
     5641 aaattcatat aacactgcta ctgccgcaac tatgccagct gtttctgacc ttaatattcg
     5701 cttgcctaaa gaaactgaag ttgcaccgca atctactgca tcagaaactt cctgtggtgt
     5761 aaacccacct atcggaccta taat
//
LOCUS       DASCNS010000230         1857 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001790811, whole genome shotgun sequence.
ACCESSION   DASCNS010000230 DASCNS010000000
VERSION     DASCNS010000230.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001790811"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="QF838_08380"
     CDS             <1..821
                     /locus_tag="QF838_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014628.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HJO60733.1"
                     /translation="KRGLLLPLYSLKQRFKTRGRILSVILSEEKMSRESFLNRVKEAL
                     SSGSNGETDLSKKLLIDDNIDDLIERTEAIRYQSRTDWMELFDTLKISSQNAGWKVHR
                     CDSYDDASDYIASLAFERNLTHIVISDQESTGRLNLGKGNLPPKSEITVVKPNDQNPS
                     FNRLQAVNADIGVTGVDYAIAETGTCVVSASNEVGRLVSLAPPMYVAVVGKGQVLPSL
                     DELFTLQRKDFVDGNSSSYTSLISGPSRSADIEYTLVTGVHGPGEVHLLLVEED"
     gene            complement(823..>1857)
                     /locus_tag="QF838_08385"
     CDS             complement(823..>1857)
                     /locus_tag="QF838_08385"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HJO60734.1"
                     /translation="VLCPVADGGDGTLETLVEVSGGEVRTCSVQNPIGETIQAQWGAM
                     GDGVTAVIEMARTSGLAFLSLAERDPLNASTYGLGQAISEALDEGFRKFIVGIGGSAT
                     NDAGAGMAQALGANLLDGYGNAINFGGAALANLQTVDISNMDSRIKKSKFLVACDVSN
                     PLTGLEGASAVYGPQKGATPKMVRSLDDALSNFATVVKKDLKKDVAEISGAGAAGGLG
                     AGMMAFMGAELKAGVDIVLDTVNLRDKLSSVDLVITGEGGMDFQTVYNKAPIGVARIA
                     SEHNIPTIAIAGLLGSNFKIVHEHGIRAATSIVNGPITLEEASERAFELISDSVEESL
                     RFISVGMDLV"
BASE COUNT          543 a          455 c          352 g          507 t
ORIGIN      
        1 aaaaaagagg gttgctgtta cctttatact cacttaaaca acgttttaaa actaggggca
       61 gaatattatc tgtaattctt agtgaggaga aaatgagtcg cgaaagtttt ttaaatagag
      121 ttaaagaagc tttgtcatcc ggttccaatg gtgaaactga tctcagcaaa aagctgctaa
      181 ttgatgacaa tatcgatgat ttgattgaac gaaccgaagc cattcgctat caatctcgca
      241 ccgactggat ggaactattt gacacactta agatttcttc acaaaacgca ggctggaaag
      301 tacacagatg tgatagttat gacgatgcat ctgactacat agcgtcatta gcgtttgaaa
      361 gaaatttaac ccatattgtc atttcagatc aagaatccac aggaagatta aatctgggga
      421 aaggtaactt accgcctaaa agtgaaataa ctgtcgtgaa acctaacgat caaaaccctt
      481 cttttaatag gctgcaggct gtaaatgctg acattggtgt tacaggggtt gactatgcca
      541 tagcagaaac tggtacatgt gtcgtctcgg caagtaatga agtaggtagg ctggtaagcc
      601 tggctcctcc aatgtacgta gctgttgttg gcaaagggca agtactacct tcgttagacg
      661 agttatttac tctccaaaga aaagattttg ttgatggcaa ctcgagttcc tacactagct
      721 tgatttcagg gcccagtagg tctgcggata tcgaatatac cttggttaca ggagttcacg
      781 gaccgggaga agttcattta cttctcgtag aagaagactg atttaaacta aatccatgcc
      841 aaccgatatg aaccgaagac tttcttccac agaatcagat atcaattcga aagccctctc
      901 tgatgcctcc tcaagagtaa ttggcccatt cactatagag gtagcagcac gaataccatg
      961 ctcatgaact atcttgaaat tactcccaag caatcctgca atcgcaatag tcggaatatt
     1021 atgctctgaa gctatccgag cgactcctat tggagctttg ttataaacag tttggaaatc
     1081 cataccaccc tctcctgtta ttacaaggtc aactgaggag agtttatctc ttaaattaac
     1141 agtgtctaac actatatcca cgccagcctt taactccgca cccataaacg ccatcatccc
     1201 agctcctagg ccaccagcag caccagcacc tgaaatttct gcaacatctt tctttaagtc
     1261 ctttttcacc actgtggcga aattggaaag ggcatcatcc aaactccgaa ccatctttgg
     1321 tgttgcacct ttttgtggtc catatacagc cgatgcccct tctagcccag ttaatgggtt
     1381 acttacatcg catgccacca aaaatttgga tttttttatg cgactatcca tattggaaat
     1441 atccactgtt tgcaaattcg ctaaagcggc tccaccaaaa ttaatagcat tcccgtagcc
     1501 atccaacaaa tttgctccaa gcgcctgagc catccctgcc cctgcatcgt ttgtcgcgct
     1561 tcctcctatt cctactatga acttacgaaa accctcatcc aaagcctcag atatcgcctg
     1621 tcccaaccca tatgttgacg cattcaaagg atctctctct gctaggctaa gaaatgcaag
     1681 ccctgaagtt cgggccattt ctatgactgc agtaactcca tcacccatcg caccccattg
     1741 ggcctgaatt gtttcgccga ttggattctg aacgctacag gtcctaactt cacccccaga
     1801 tacttcaacc aaagtctcca aggttccatc acctccgtca gctacgggac aaagtac
//
LOCUS       DASCNS010000231         7942 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001792527, whole genome shotgun sequence.
ACCESSION   DASCNS010000231 DASCNS010000000
VERSION     DASCNS010000231.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001792527"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="QF838_08390"
     CDS             complement(<1..1585)
                     /locus_tag="QF838_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60735.1"
                     /translation="MWPKMLNRRVALGIAAAALIGAGIACSSSDDDAAPAAAPAAAPV
                     AAPAAAPAAAPAAAPAAAPAAAPAAAAAKPAAAPAAAPAAASSGGPNGHVKLAAADIN
                     SPNGLPRFCTAGCAETIYMSGITDVLFNAVADGGVVTTEPMVALSYELDPSLESGIFT
                     LRQGVQFHGGYGEMTAKDVEFSYNDANSVTNPESIHGQAGDFAPLISSMEAVDDYTLK
                     LNYRNYDSRGILHRFSSFWQTAGIMSTAVFAEHGVEGMQDIYVGVGPFENSEWSQNGK
                     IEGTAFADYYGTVGPEKMTLLEVPEGASRRAMLETGEVAISQVATKDYPGLSDKGFNA
                     QKGGMFNTIRDVSMVGNYWETHNALTGAELTRDRDTSLPWIGNPFENGDYDETTPSMV
                     SSQKVREAFAFAIDREALVENLLGGLGFVNHQPYLAGSNPNYQADWDFGLDYDAAKAL
                     MADAGQSGGFEMDLWVGTGELGAEIGESVGAAWQENLGVKVNLIKTAYSTYRPGLVAR
                     TNKTPGVNICGDENKSNFPY"
     gene            1905..2810
                     /locus_tag="QF838_08395"
     CDS             1905..2810
                     /locus_tag="QF838_08395"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HJO60736.1"
                     /translation="MAAYEGIFPAIVTPMSSDGTINESAFREVMEFNIQAGVGGFWVA
                     GGNGESVLLSDNENMRIADIASDQNRGRINNIMHVGAPTTKSAIKLAEHAAKAGAEAI
                     CCVPPFFYRQTDEAIVEHYKAVAASADLPFYVYNLPQSTGVEITVELMQKIQDAVPQL
                     AGLKHSSINFANVREWVKMDLKCFIGSSMLMVPALSIGAVGCIDGPPCAAPELWVNIW
                     KSWQAGDIDEAMNAQRKASSFTEIMREAGVHSGTKAVLTERLGIDCGSGRLPNPPLEG
                     KRRENILQRVSDLGIGAVMSMASDD"
     gene            2909..3334
                     /locus_tag="QF838_08400"
     CDS             2909..3334
                     /locus_tag="QF838_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60737.1"
                     /translation="MERIFALFIRAGLAAIFGFMFGAMFMIGTFWVVPPIIILPMWLL
                     SISVGFGCGLAGFICFLKPEAKMTINLTTFLIACLSGVIGGYLGSIMSDPEGVRNVRL
                     VASSVTSPDVTPFIYMGTFISTAATSAWYAYRLWLYNED"
     gene            3430..4140
                     /locus_tag="QF838_08405"
     CDS             3430..4140
                     /locus_tag="QF838_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HJO60738.1"
                     /translation="MSSTNLDSVTTLTFDIFGTVLDLAGSLIPPLNRLLTACDAPSAL
                     TGEQVWAQWRLRQRIEQYQDNMLMLGHSGYLPVKRKALMYTLRSLKVDFTSENLDEFM
                     KAYQLLTPFKDAIDGLIRLSNKYRLVMLSNGEHHYLEYLAKNRIGIDFDQILSAETVS
                     QFKPHPSVYRFAAKSLNLEPQQIMMVAAHSFDILGARHSGFRGAYVNRYDLPYDESDY
                     IPDITTKNFYDLCDTLEV"
     gene            4157..5101
                     /locus_tag="QF838_08410"
     CDS             4157..5101
                     /locus_tag="QF838_08410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60739.1"
                     /translation="MIKNYDKVRIGMAMSGWPFLNNDPSQLADYVIEMERLDIDSLWF
                     TDRIVSETFTLETVVAMSFVAALTEKLKFGTSVIGLPLRNPTILAKEIATLDYLSNGR
                     SLPAVGLGPETENDYEACGTDKKSRVGRTEEAVEIMRLLWSGNNVSYKGNHFTLSNVS
                     IEPKPVQKDLPPIWFGGRSEPAINRTAKIGDGWLVSYATAEEVSISVPKIKGKALEYG
                     NHIEDDHYGALISFCFADTRQEAKKLAENYNVVRRADVDLKDVTAFGPPDILINLLDD
                     FIKAGATKFTLRSACPPEMTFDQMRLLGKYIVPRYHNG"
     gene            5094..6119
                     /locus_tag="QF838_08415"
     CDS             5094..6119
                     /locus_tag="QF838_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60740.1"
                     /translation="MAESSFFLIGVRFSRYLRIVFWGILLVCVTSCSYEKLYIVYDDG
                     IYLHRVDPDNPRPELLLPGVGAISPDASTDSQLLTFISNNGSGQLVGLWNGSSKIKWH
                     KHPNFDPYIFDPSPINSSSYIFSGLHSDYLDIFSSVQGKNITNFRSMDFEPSVSPNGN
                     TLAFTSSRISNSDIYTNERPLSDRQAIETVKEWDLTERGIKYNTYRDHNIDIFTANID
                     GTNTVRMTFARALDHQPDWSHDGDWIVFSSLRHNNSEIFKIRKDGTGLKRLTNNLHND
                     GEPAWSPDGNSIAFTSDRSGSKNIHVMTPDGANQIQLTNTLSNLHSPTWIKCSPGIFG
                     KVGTTCP"
     gene            6110..6943
                     /locus_tag="QF838_08420"
     CDS             6110..6943
                     /locus_tag="QF838_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO60741.1"
                     /translation="MPLNSVKVGNFYIHSVSDGYMIFQRDAFFPNLPIESWKPYPSYS
                     DPKFEMNIGSFVIEGADKTILVDTGLGKLNHHIEQPMRETLLSELEAFSIKPTDIDIV
                     FLTHLHLDHVGTNMTRDNGVWKQTFPNAQYMVGKSDWEMFSRMINNPSFKYLEEQVQP
                     LLSNGSLYLFEGEIPLTEGVVTIPTPGHTPGHTSLLIESDGAKAVIMGDTVHIPPQVE
                     QISWSPVPDRDKALSAETRSDLMDFIEKEQALIISGHFPKPGFGEIIQVGSTRSYRPI
                     T"
     gene            complement(6960..7202)
                     /locus_tag="QF838_08425"
     CDS             complement(6960..7202)
                     /locus_tag="QF838_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60742.1"
                     /translation="MISTIILTGSCPRCGTGTLTNDEDMFGVRLFCVMCGHNRDLGKL
                     PVKVKNIRDLVLAVLDSDRIALQKQRAATYPPSVYG"
     gene            complement(7302..>7942)
                     /locus_tag="QF838_08430"
     CDS             complement(7302..>7942)
                     /locus_tag="QF838_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60743.1"
                     /translation="TNFLFDTLIPAAEYIQVDKGFALVASLTPGTHEIMYSYDLPYNG
                     QEAEVIKSWRYGVENASILYPNGTVNINTNFETKSQDTIGGKAYTILESKNIAKGAKT
                     TIVLKDLPTPRFFDRISNQFGQMRYEYTGPIGLLLVLISIGTIGIFNTIRLRNRRESW
                     LVGSSESQVILDQIAELDRRFNEDKLDNRLYIDRRDYLVRRLQTISEETEVE"
BASE COUNT         2316 a         1752 c         1698 g         2176 t
ORIGIN      
        1 catatgggaa gttcgacttg ttctcgtcac cgcaaatgtt gacaccaggt gtcttgtttg
       61 ttcgtgcgac aagtccaggt cggtaagtgg agtaagcagt cttgatcaaa ttgaccttga
      121 ctcccaaatt ctcctgccaa gcagctccaa cggattctcc gatttcagca ccgagttcac
      181 cagtacctac ccagaggtcc atttcgaatc cgccactttg accagcatca gccattaagg
      241 cttttgccgc atcatagtcg agaccgaagt cccaatcggc ttggtagtta gggttgcttc
      301 cagcaagata tggctgatgg tttacgaaac ctaggccacc aagcaggttc tcaacgagag
      361 cctctctgtc aatcgcaaag gcgaaagctt ctcgaacttt ctgcgaactg accattgaag
      421 gtgtagtttc gtcgtaatcg ccattctcaa atgggttacc aatccatggt agagatgtat
      481 ctcggtcacg cgtcagctca gcaccggtta gtgcgttgtg agtctcccag tagttaccta
      541 ccatcgatac gtctctgata gtgttgaaca ttccgccctt ctgagcatta aagcccttat
      601 ctgataagcc aggatagtcc ttggttgcta cctgtgagat tgcaacctca ccagtctcta
      661 acatagctcg ccttgaagca ccttctggaa cttcaagaag agtcattttc tcgggaccga
      721 cagtcccgta gtaatcagcg aaggcagtac cttcgatctt accgttctga ctccactctg
      781 agttttcaaa agggcctaca cctacataga tgtcttgcat cccttctaca ccatgttctg
      841 caaaaaccgc ggtgctcatg attccagctg tctgccagaa gctactaaac ctgtggagaa
      901 ttccacggga gtcatagttt ctatagttca gctttagagt gtagtcatcg actgcttcca
      961 tgctcgaaat caacggagca aagtcaccag cctgaccgtg aattgactct gggttcgtta
     1021 ccgagttcgc atcgttatac gagaactcaa cgtccttggc agtcatttca ccgtatccgc
     1081 catggaactg aactccttgt ctgagtgtga aaattccaga ttctaaggaa gggtctagtt
     1141 cgtatgacag agcaaccatt ggctcggtgg tgacaacacc accatcagca acagcattga
     1201 aaagcacatc agttataccg gacatataaa tagtctcggc acatccagct gtacagaacc
     1261 taggcaatcc gtttggcgaa ttgatgtctg cggcagctag tttgacatgc ccattcggtc
     1321 cacctgagct tgcggctgca ggagcagcag caggggcagc agcaggttta gcagctgcag
     1381 cagcaggtgc ggcagcaggt gcagcagcag gtgcggcagc aggtgcagca gcaggtgcgg
     1441 ctgcaggagc agcgacagga gcggcagcag gtgcggcagc aggtgctgca tcatcgtcag
     1501 aagagctaca agcaataccg gcgccgatta acgcagcagc ggctatgcct aatgcaactc
     1561 tcctatttaa cattttaggc cacataagtg atcctccttc tactttggtc ttttgaccag
     1621 aatagccaac cttcgccttg tcccaaatag aaaaaggcgt gggaaattag accatatcgc
     1681 tttcttcaaa gtcaacactt tgatgcacat atctacccat gaaagtctgg tatttcatca
     1741 ccgcccccta tataccatca ccgcccttcc taaaaactat ctcaatatca atagtctaaa
     1801 ctcaattttg aaacacgtgc tagaatcctc ccaatgtctg gtttttaaat tgttcaacaa
     1861 aatttcacag actaaatcag caccaagaat taaggagtaa agcgatggca gcttatgaag
     1921 gtattttccc ggcaattgtc acaccgatgt cctctgatgg aacaataaac gaatcagctt
     1981 tccgtgaagt aatggaattc aatattcaag ctggcgtggg agggttttgg gtagcaggtg
     2041 gaaacggaga aagcgtgttg ctatcagata acgaaaacat gagaattgca gatatcgcct
     2101 ctgaccaaaa cagaggaaga atcaataaca tcatgcatgt tggggctccg actaccaaga
     2161 gtgccataaa attggctgag cacgcagcta aagcgggtgc tgaagctatc tgttgcgtac
     2221 ctccattttt ctacaggcaa accgatgagg caatcgtgga acattacaag gctgtagcag
     2281 catcagcaga tttacctttt tatgtttata acctacccca atcgacagga gtagaaataa
     2341 ctgttgagct aatgcaaaaa atacaagatg cggttccaca gttagcagga cttaagcatt
     2401 cctctataaa ttttgccaat gtaagagaat gggtaaaaat ggaccttaag tgctttattg
     2461 gaagtagtat gctaatggtt cctgccttgt ctattggagc cgtcggttgc atagatggac
     2521 ccccatgtgc agctccggaa ttatgggtaa acatttggaa gtcctggcaa gcgggtgata
     2581 ttgatgaggc gatgaatgct cagagaaagg cctcctcttt cactgaaata atgagggaag
     2641 ctggtgttca tagtggtaca aaagccgtgc tgacggaaag gctaggaatc gattgtggaa
     2701 gtggaaggct tccaaatccc cccttagaag gcaaaaggcg ggaaaacatc cttcaaagag
     2761 tatcggattt aggaattggt gcggtgatgt ctatggcatc ggatgactaa tcataaccgt
     2821 gattagcctt ttatcgtaaa aagttgggag cttaagtcaa ggagcccagc ttattagtta
     2881 atatataaaa tgaataagga atccgactat ggagcgaatt tttgctcttt tcatacgtgc
     2941 cggtctagcc gcaatttttg gctttatgtt tggggcaatg ttcatgatag gcactttctg
     3001 ggtagtcccc ccaatcatca ttctccctat gtggttatta tcgatatcag taggattcgg
     3061 ttgtgggctt gccggcttta tatgttttct caaaccagaa gcaaaaatga cgatcaatct
     3121 aactactttt ctaatcgctt gtttaagcgg tgtaatcgga gggtacctgg gttcaattat
     3181 gtcagaccca gaaggtgttc gaaatgtgcg cctggtagct agttccgtta cctcgccaga
     3241 tgtcacacct tttatttata tgggaacatt catatcaaca gcggcgacat cggcctggta
     3301 tgcatacaga ctctggttgt acaacgagga ttagtatcaa gccttattca ggcaattatt
     3361 ttcaaatgca aatttggact actaagctaa aatacttctt aataaatata ccttgaggag
     3421 accattggaa tgtcctctac caatttagat tcagttacca cacttacatt cgatattttt
     3481 gggaccgttc ttgatcttgc gggaagtttg attcctcctc tcaaccgttt actgacagct
     3541 tgcgatgccc cgtctgccct aaccggagaa caggtctggg cacagtggag gttaagacaa
     3601 agaatcgaac aataccaaga caacatgctt atgcttgggc acagcggtta tttaccggtc
     3661 aaaagaaagg ccttaatgta cacattacga agcctaaaag ttgattttac gtctgaaaac
     3721 ttagatgaat tcatgaaagc ttatcaacta ctgactccat ttaaagacgc gattgatgga
     3781 ttaataagac tgtcgaacaa atacaggtta gttatgcttt ccaatggtga acaccactat
     3841 ctagaatatc tggcaaaaaa caggatcgga atagatttcg accagatttt gtctgcagaa
     3901 actgtcagcc aatttaaacc ccatccgtcc gtgtatagat ttgctgcaaa aagtttaaat
     3961 cttgaacctc aacagatcat gatggtggcc gcgcattctt tcgacatttt gggagcaaga
     4021 catagtggat ttagaggagc atatgttaat cgctacgatt tgccctatga cgaatcggac
     4081 tatatccccg acatcacaac taaaaacttt tacgaccttt gcgacacttt agaagtttaa
     4141 tggagggtaa gggtaaatga ttaaaaatta tgacaaagtt agaataggaa tggctatgtc
     4201 aggttggccg tttctcaata acgatccgtc ccagcttgcc gactatgtga tcgaaatgga
     4261 gagactggat attgattcgc tttggttcac agaccgcata gtatcagaaa ctttcaccct
     4321 agaaaccgta gtagcaatgt cgtttgtcgc tgcccttaca gagaaattaa aattcggcac
     4381 cagcgtgata ggtttgccac taagaaaccc tacgattctc gcaaaagaga ttgctacatt
     4441 agattacctg tccaacggta gatcgttacc tgcagtaggt ttggggccag aaacggaaaa
     4501 tgactatgaa gcctgcggta cggataaaaa gtcacgtgtg ggacgaacag aagaagccgt
     4561 agaaattatg cgccttctct ggtccggcaa taatgtgagc tacaaaggta atcactttac
     4621 cctcagtaat gtttctattg aaccaaaacc agttcaaaaa gatttgccgc ccatttggtt
     4681 tggaggaagg agtgagcctg ctatcaacag aacagcgaaa attggtgatg gttggctagt
     4741 ttcctatgca actgctgagg aagtatctat aagtgtccca aaaataaaag gaaaggctct
     4801 tgaatacgga aaccacatag aagatgacca ttatggtgca ttgataagct tctgttttgc
     4861 tgatactcgc caagaggcta aaaaattagc ggagaactac aatgttgtgc gaagggcaga
     4921 tgttgaccta aaagatgtta cggcctttgg gccaccagac attcttatca atcttctcga
     4981 tgatttcatt aaggctggcg caaccaaatt tactttacgt tcggcttgtc cccctgaaat
     5041 gacattcgat caaatgcgtt tgctcggaaa atacatcgtg cctcgttacc acaatggctg
     5101 agtcttcttt ttttttaatt ggcgttcgat ttagccgata cctaagaatc gtattttggg
     5161 gaatcttact ggtttgtgta actagttgca gttacgaaaa gttgtacatc gtctacgatg
     5221 atgggatata tcttcatagg gtagacccag acaacccaag gcctgaactt ctgctacccg
     5281 gtgtcggagc gattagccct gatgcttcaa cagacagtca attactcact ttcatatcga
     5341 ataatgggtc tggccaactt gtgggtttgt ggaacggtag ttcaaaaatc aaatggcaca
     5401 aacacccgaa ttttgaccca tacatttttg atccctcacc tattaattca tcaagttaca
     5461 tattctcagg attacattcc gactatttag atatattttc ctctgttcag ggcaaaaaca
     5521 ttacaaattt cagatccatg gattttgagc cctcggtatc cccaaacggt aatactctag
     5581 cctttacaag tagcaggatt agcaattcag acatatacac aaacgaaaga cctttaagtg
     5641 acaggcaggc catagaaacg gtaaaagagt gggatcttac agagcggggt ataaaataca
     5701 acacttatcg agatcacaat atcgatattt tcactgccaa tatagatggc accaacactg
     5761 ttagaatgac ttttgccaga gctttggatc accagcctga ttggtctcat gatggtgact
     5821 ggatcgtatt cagttcccta cgtcataata attcagagat cttcaaaata agaaaagatg
     5881 ggacaggact caaaaggttg acgaataatc tccacaatga tggagagcct gcctggtctc
     5941 ccgacgggaa ttcgatagca tttacatccg atcgatcagg ttcaaaaaat attcatgtca
     6001 tgaccccaga tggtgcaaat caaattcaac tcacaaacac tttgtccaat ttacattcac
     6061 caacttggat taaatgcagc cctggaatat ttggaaaagt aggtacaaca tgcccttaaa
     6121 ttcggttaaa gtgggaaact tttacatcca ttcggtgtca gatggttata tgattttcca
     6181 gcgagacgcc ttttttccaa atttgcctat tgaatcatgg aaaccatacc cttcttattc
     6241 cgatccaaaa tttgaaatga atattggttc tttcgtcatt gaaggggccg ataaaaccat
     6301 actagtagat actggactag gaaaattgaa ccatcatatc gaacaaccta tgcgtgagac
     6361 attattgtca gagctagagg ccttttcaat caaacctact gacatagaca ttgttttctt
     6421 aacgcacctt catttagacc atgtgggaac aaatatgacc agagacaacg gagtttggaa
     6481 gcaaacattt cctaacgcac agtatatggt cggaaaatct gactgggaaa tgttcagccg
     6541 catgatcaat aacccgtcat tcaaatattt agaagagcag gtccaacctt tgctctcaaa
     6601 tggaagttta tacctgtttg aaggagaaat tcctttaact gaaggggttg ttaccatacc
     6661 aacccctggg catacgcctg gacacactag cctacttata gaatcagacg gggcaaaggc
     6721 ggttattatg ggagatacag ttcatatacc cccacaagtg gagcaaattt catggagtcc
     6781 agttccagac agagataaag cactatcagc tgaaaccagg tcagatttaa tggacttcat
     6841 agaaaaagaa caggctctga ttatatccgg gcactttcct aaacctggct ttggagaaat
     6901 tatacaagta gggtccacgc gatcctaccg acctataacc tagaggttgt gcaaccactc
     6961 taaccatata ccgagggagg gtaggtagcc gccctctgtt tttgcagagc aatacggtcg
     7021 ctgtctaaga ccgccaatac gaggtcacga atatttttaa cctttacggg taatttccct
     7081 agatctctat tatgcccgca catcacacaa aaaagcctta ccccaaacat gtcttcatcg
     7141 ttggttaggg taccggtacc acacctaggg cacgaaccag ttagaattat tgtacttatc
     7201 atctttttct aaaatatctc tacattttca attgtattga tagactgatt tcatatagaa
     7261 accacttctt acaatacacg aactttatcg aaaaagctgt cctattcgac ctcagtttct
     7321 tcagaaattg tttgcagtct tctcaccaga tagtctcttc ggtctatata tagcctgtta
     7381 tctaatttat cttcattaaa ccgcctgtct aactcagcta tttgatccaa aataacttga
     7441 gattcactgg aacccacaag ccaggattct cgacgattac gtaaccgaat ggtattaaaa
     7501 ataccgatgg ttccgatgga aatcaaaacc agtagtaacc ctatcgggcc ggtatattcg
     7561 tagcgcattt gcccaaactg attagaaatg cgatcaaaga atcttggggt cggtagatcc
     7621 ttcaaaacta tcgtggtttt cgcacctttt gcgatatttt tagattccaa aattgtgtat
     7681 gcttttccac caatggtatc ttggcttttt gtctcgaaat ttgtgtttat attaaccgta
     7741 ccgttgggat ataaaatact ggcattttca accccatacc gccaagactt gattacctcg
     7801 gcctcttgac cgttgtatgg aagatcatag ctatacataa tttcatgagt cccaggagta
     7861 agagaggcaa caagggcgaa tcccttatcc acttgtatat attctgccgc tggaattaaa
     7921 gtatcaaaga gaaaatttgt cg
//
LOCUS       DASCNS010000232         7901 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001805833, whole genome shotgun sequence.
ACCESSION   DASCNS010000232 DASCNS010000000
VERSION     DASCNS010000232.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001805833"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            447..1574
                     /locus_tag="QF838_08435"
     CDS             447..1574
                     /locus_tag="QF838_08435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HJO60744.1"
                     /translation="MIVKQKGEVVDKDNLITLTDYEKASYGKMSKELADYVRSGAQDE
                     ITLRRNRQALEDIIVNPRIFRAVDYRDLSTTVLGDKIDIPIMLTPAGGQLHMHPDGEL
                     ASSRASYRHGTVYSTPTNSGYSLEKIADETPGPKWFQIGQHSREIQEHFVKRAKQAGY
                     KAIVLTADAPIPSRRESEIRNNFSIQNHLSWGSVEGNEEFLLSDPHLRKYIYGMSEKE
                     EPVSAGTGDKSMTWDDIEWFRGLTDLPVIVKGVRNIEDAKRCADVGFDGITVSNHGAR
                     HMDGTNSSIEVLAEIAPAVNERIEVFFDSGIRRGMDVAKALCLGARAVLVGRPIFWGL
                     CVDGEQGVFSVLDILKTELDLAIAYMGVNKVDDLNRSYVTI"
     gene            1661..2884
                     /locus_tag="QF838_08440"
     CDS             1661..2884
                     /locus_tag="QF838_08440"
                     /inference="COORDINATES: protein motif:HMM:NF018485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60745.1"
                     /translation="MAIKNAWFTLTVAFIVLFFSSGLRFAFGIVLKPMSEDLGWARAE
                     TTMAATTFFVVSALVMPIYGRLIDRYSLIQIMIISIAITSLGFGLVAIVQTPWQFIFF
                     YGIVFALGHSGFSILAVSVMVGRAFPNRAGIANSIAMSGFGFGQLIIISVLTLIVFEV
                     GWRISHVYLALGAALVLIPLLLFQAKFVSFLPDSPDKYEGHPTEFSSSSVPHLLRFTI
                     STKFALLIVVYALCGFQDFFMATHIASFATDNGVSDQFAGNLLAIMGMFALAGVITAG
                     LLGDRFKAGFPTVLCFLIRLVIFILICTSNQPAFIVILAFSYGFTFLITAPLTVVFVK
                     DLFGTPKMGALIGIVNMVHQFGGAIGAYAGGVVFDQTGSYLLMMIFMVVLSLGGLICS
                     YFIHGSATFRKRLVN"
     gene            3150..4469
                     /locus_tag="QF838_08445"
     CDS             3150..4469
                     /locus_tag="QF838_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HJO60746.1"
                     /translation="MSKQPNIVLIMSDDLGYEVIGANGGSSYKTPSIDSMAQQGMRFE
                     NAHVMPLCTPTRLSMMTGKYNFRNYLGFGLIRPDEVTFGHLLTDAGYNTCISGKWQLY
                     SYNPPDQHPEERSSGQKIEDAGFDEFCVWHPHHTEEKGSRYKSPVIYENGEFRTDTKD
                     KYGEDIFCDYIIDFLERKKDDEKPFFVYWPMAATHKPHEPTPDSPEWDSFDPPSNRSI
                     GAKTWADLEDASWEDDPKFYRDMVEYHDKVIGRLLNYLENQGLQEDTLVIYLGDNGSP
                     ADVCSMMHQHNEICGGKGKTSDRGTHVPLICLMPGTIPSNTVQTDLIEATDFLPTIFE
                     AAGLNFPEGYIIDGRSFYPQLKGEKGNPRDWMFFHFEPMNRRHGLNDLRQIRFIRDHR
                     WKLYETGELYDLEADWDEDIPIYEVEDSKSQSEARNRLNPVFSQMTN"
     gene            complement(4478..4870)
                     /gene="msrB"
                     /locus_tag="QF838_08450"
     CDS             complement(4478..4870)
                     /gene="msrB"
                     /locus_tag="QF838_08450"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HJO60747.1"
                     /translation="MADLKDDEQYRDKLTPMQYEVTRNKSTERAFTGEYWNTTEDGTY
                     LCVCCGEELFSSDTKYDSMCGWPSFYQPLVEDQIEEETDTSLGMERTEVICKSCGAHL
                     GHVFPDGPQPTGLRYCINSASLNLKKDE"
     gene            5009..6142
                     /locus_tag="QF838_08455"
     CDS             5009..6142
                     /locus_tag="QF838_08455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60748.1"
                     /translation="MALQFGVFDHIEPVSNMSLNEVYKLRMQQIEVLDQKGFYAYHLA
                     EHHTPAVHSLAPSQNVFLAAASQRTSQIKLAPCVYVLPLHHPLRLIEEISMLDHLSDG
                     RVEIGVGRGGVMEAFFWGQDDDSDKNYQRYLETLEVVKEGLSSETLTYQGQFHQYDNL
                     PMRLEPIQKPFPPMWYMRNAETAALEGMNTVIVGTLESLKAETKRYKDVWNKTHGEGS
                     LNDQGQDPKIGLVVHMVVARTDEEAINIATPAWEKYKWNLAAPRRIEAEKRGLEQFQS
                     SKDGSFGFVGDRPASLPTRETRKDIEAEIERFDQQSNRKDPTRLGGVALAGSPKSVER
                     YLEEYIETGANYFVCSFQWGDIDHETAMNSINLFSEHILPQYI"
     gene            6161..7288
                     /locus_tag="QF838_08460"
     CDS             6161..7288
                     /locus_tag="QF838_08460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60749.1"
                     /translation="MKLEIIGMIGAVREGTGSKGAEVHIIGGGVDGDYISDFAKAHEN
                     SGFDKVLVGYTSSSADGFIVAMHAAANTEELGFLVAHRPGFVSPTVLSRKAATIDQLT
                     QGRIALHIISGGGDLEQRRDGDFLDHDARYRRSGEFMHILKTLWTSKTPVDHKGEFYQ
                     FEQAKSDFECFQKPHIPLYFGGASDAALEVGARECNVYAMWGEPLKEVEKKIRLFTDR
                     VTRVGRDPKSVRFSVSTRPIVADTESEAWDIAHSTLENVLKTNQNSMHGMGKERLESV
                     GSQRLIDFAKDGEILDDRLYTPIAAATRGAGNTTALVGTAGQVSDSLMKYYKLGCSTL
                     LVRGFDAYNDAVYWGKELIPNLKESVANYHKRHAVTGKHLL"
     gene            7285..7797
                     /locus_tag="QF838_08465"
     CDS             7285..7797
                     /locus_tag="QF838_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HJO60750.1"
                     /translation="MKILSAVLGSPFQGSSSEKIVNIFIDRLPKERWDTALVDLSKIS
                     SEALLLRSKDKALNASIERVLNSDVIIAATPTYRATYTGLIKTFFDQFPENSLSGKLV
                     LPIQTGGSAEHALSVEHGLTPMVRTLGAVVANKSIYSWGEHWNEDGNPGQNLKEMIDQ
                     SVNESINTSG"
BASE COUNT         2374 a         1550 c         1783 g         2194 t
ORIGIN      
        1 tcttaagggg gccggggaca ctgctttcat tatgttaacc atatgtctca cagggctcat
       61 ggccctcata gcacctacat ttctaatcat tgaaatcttc cacctgggct tgaatgttgc
      121 atggatttca ggtgtcgcgt atgtactctg ccttggaatt gtgtattacc tcaggtttaa
      181 aggcggcaaa tggcaaagta tgagggtgat agaaaaccgg caggcaacct aagatgaaag
      241 agtaagtcga tatattggtg aaaaaaagtt aggctgttca tgtcaggcga taggaaccga
      301 aaagtggttt gatggattta taaagtgtct acaccgatga atcagagtgc tcactggatt
      361 gccttcacat tagttttggt gttgttttag ttttgattta taggaattca cagaaacttt
      421 tatctacagc tggataagga atcaaattga tcgttaaaca aaagggagaa gtcgtggata
      481 aagataatct aataacactt actgattatg agaaagcttc ttacggaaaa atgtctaaag
      541 agttggctga ctatgtgaga tctggggctc aggatgaaat aactcttcgg agaaaccgac
      601 aagctctaga agacataata gttaatccca ggatatttag agctgtagat tatcgtgatc
      661 taagcaccac ggttttgggc gataaaatag atattccgat tatgctaacc cctgcaggcg
      721 gacaacttca tatgcaccct gatggggagt tagcctcgtc cagagcatct taccggcatg
      781 ggaccgtcta ctcaactcca acgaactctg ggtactcatt agaaaaaatt gcggatgaga
      841 cacccggtcc taaatggttt caaattggac aacacagcag agagattcag gaacattttg
      901 tcaaaagagc aaagcaggct ggttataaag ctatcgtcct taccgcagat gccccgatac
      961 caagtaggag agaaagtgag attagaaata atttctcaat tcaaaatcat ttgagttggg
     1021 gcagtgttga gggtaacgaa gaatttctgt tatctgatcc ccacttacgc aaatatatat
     1081 atggcatgtc agaaaaagaa gaaccagtat cagcaggaac cggtgataaa tcaatgacct
     1141 gggatgatat tgaatggttt cgtgggctaa cagacctgcc cgtaatcgtt aaaggagtaa
     1201 gaaacattga ggacgcaaag cgttgcgcgg atgtagggtt tgatggaatt acggtttcta
     1261 atcatggtgc tcggcacatg gatggaacaa attcgtccat agaggttcta gcggagatag
     1321 ctccagcggt gaatgagcgg atcgaggtat tctttgactc aggaattagg agaggtatgg
     1381 atgttgccaa ggcgctatgt ttgggagcta gagcggtcct tgtaggccgc ccaatcttct
     1441 ggggattatg tgttgatggt gaacaaggtg tatttagtgt gctagatatt cttaaaactg
     1501 aactcgatct tgcaatagct tatatgggag taaataaagt agatgattta aataggtcgt
     1561 atgtcacaat ttgaagtgtg aagccatcgt gattttccaa gaatagataa tctaagaacc
     1621 tcagtatttc atccacagat accttcataa ggggggttat ttggcaatta aaaatgcttg
     1681 gtttactctg actgttgctt tcatagtttt atttttttcc agcggtctga gattcgcctt
     1741 tggaattgtg cttaaaccta tgtctgaaga cctgggttgg gccagagcag agacaacaat
     1801 ggctgctact actttttttg tagtgtctgc cttagtcatg ccaatatatg gacggctaat
     1861 cgaccgttac agtcttatac agataatgat tatatctatt gcgataacct cgcttggatt
     1921 cggcttagtt gcgattgtcc agactccatg gcagttcata tttttttacg gaatagtttt
     1981 tgcattaggt cactcagggt tctcgatttt ggctgtgtcc gttatggtag gacgggcatt
     2041 tccaaatcga gctggtatag ccaatagtat tgccatgtct ggattcgggt tcggtcagct
     2101 catcattatc tctgttttga cactaattgt gtttgaggta ggttggagaa tatcccatgt
     2161 atatttagct ttaggggcag ctttagttct tattcctcta ctgctgtttc aggctaaatt
     2221 cgtgagcttc ttgccagata gccctgacaa atatgagggc catcccaccg aattttcatc
     2281 cagttcggta ccacatttac taagattcac tatctcaaca aaatttgccc ttttgatagt
     2341 agtttatgcc ttatgcggat tccaagactt tttcatggct acacatatag catctttcgc
     2401 aactgataat ggtgtcagcg atcaatttgc gggtaatttg ttagcaataa tgggtatgtt
     2461 tgcacttgca ggagtgataa ctgcagggct cttaggtgac aggtttaaag ctggttttcc
     2521 tactgtgctt tgttttttaa tacgattggt catattcatc ttgatatgca cttctaatca
     2581 gccggcattt attgtgatat tagcattttc gtacggattc acattcttga ttactgcgcc
     2641 tctcacggta gtatttgtaa aagatctttt cgggacacca aagatgggag ctcttatcgg
     2701 tatcgtcaat atggtgcatc aatttggagg tgctattgga gcatatgcag gaggcgtagt
     2761 tttcgatcaa acaggatcat acctattgat gatgattttt atggtagtac tatctttagg
     2821 tggactaatt tgttcatact ttatccacgg gtctgcaact tttagaaaaa ggttggtcaa
     2881 ttaactagtg ggaatattca cctcgttgcg agttgaaatt ttcccgatta tgacctactt
     2941 tcagtctgtt tgctatgatt aaattgcatg catactaggt tgtgccttag catcaagagc
     3001 tttgttagat gaatcagagg ggtcaatatg aaaattaagt ctgtgaaagg cctgttgatt
     3061 tgaagccggc tcctacaaat aaaccgcaag cacaacctga tccaatatac caaatatgat
     3121 agtttagaaa cctacaaggc ggagccccta tgtcaaagca gccgaatata gtattgataa
     3181 tgtctgatga tcttggctat gaggtaatag gggccaacgg tggttcttca tataaaactc
     3241 catccataga ctcgatggcc cagcagggca tgcgctttga aaacgctcat gttatgccgc
     3301 tgtgtacacc aaccagactt tcaatgatga ccggaaaata taatttccgg aactacttgg
     3361 gtttcggttt aataagacca gacgaagtca cttttggaca tctactcacc gacgcaggtt
     3421 ataacacatg tatatccggc aaatggcaac tttactccta caacccacca gatcaacacc
     3481 cggaagaaag atcctcaggg caaaaaatcg aagatgctgg ctttgacgag ttttgcgtat
     3541 ggcacccgca tcacacagaa gagaagggtt ccaggtacaa gagtccagtt atatacgaaa
     3601 acggggagtt cagaactgac accaaggata aatatgggga ggacatattc tgcgattaca
     3661 tcatcgattt tttagagagg aaaaaagatg atgaaaaacc atttttcgtc tactggccaa
     3721 tggctgctac ccacaagcct catgagccca ccccggacag cccagaatgg gattcttttg
     3781 atcctccctc aaaccgatct atcggtgcaa aaacatgggc ggacttagaa gatgcatcat
     3841 gggaagatga tcctaaattc tatagagata tggttgagta tcacgacaaa gtcataggta
     3901 gattactgaa ctatcttgaa aatcaaggac ttcaggaaga tacattggtc atctaccttg
     3961 gagacaacgg tagcccagcc gatgtctgtt caatgatgca ccagcataat gagatttgcg
     4021 gtggtaaagg aaagaccagc gataggggta cccatgttcc tttgatttgt ttgatgccag
     4081 gaacgattcc ttccaatact gtgcaaacag atcttattga agctacagat tttctaccga
     4141 ctattttcga agcggctggc ctgaacttcc ctgaaggata catcattgac gggaggtctt
     4201 tttatcctca gttaaaaggg gaaaaaggca acccgagaga ttggatgttt tttcacttcg
     4261 aaccaatgaa tagaagacac ggcctcaacg atttacgaca aataaggttc attcgtgatc
     4321 acagatggaa attatatgaa acgggggaat tatacgattt ggaagcagat tgggacgaag
     4381 acatccctat ctatgaagtt gaagattcaa aatcccagtc tgaagcaaga aaccgactaa
     4441 acccggtatt ttcccaaatg accaattgat ccttaatcta ctcgtctttt tttagattta
     4501 aggaagctga gttaatacaa taccgcagcc cggtaggttg aggaccatct ggaaaaacat
     4561 gccccaggtg cgcaccacat gacttgcaga taacttccgt cctctccatt cctaaagaag
     4621 tgtccgtttc ttcttctatc tgatcctcaa caagcggctg gtaaaagcta ggccacccac
     4681 acatggagtc gtatttggta tcagaagaga aaagttcttc cccacaacac acacataaat
     4741 aggtaccatc ctctgtcgtg ttccagtact cccctgtaaa tgctctttct gtagatttat
     4801 tacgagttac ttcatattgc atcggagtaa gtttatcccg gtattgttca tcgtctttta
     4861 aatcagccat ctatccgtct cccaaaattt tgaatactgc tacagtacat tttcagcaca
     4921 acacagaaga ttatatagct agtattatat tggttcacaa attttgtcgc cggtaaaact
     4981 gatagcacac aaaacttagg agagttattt ggcacttcaa tttggcgtat ttgatcatat
     5041 agaaccagtc tcgaacatgt ccctaaatga ggtttacaag cttcggatgc aacaaatcga
     5101 ggttttagac caaaaagggt tttatgcata ccatctagct gaacaccata cacccgctgt
     5161 gcatagccta gctccttctc aaaacgtatt tttagccgca gcatcgcagc gcacgtcaca
     5221 aataaaacta gctccatgtg tttatgtatt gcctcttcat cacccactta gattgatcga
     5281 agaaattagc atgcttgacc acttgagcga tggacgggta gaaatcggag tcggaagggg
     5341 aggtgtgatg gaagcttttt tctggggaca ggatgacgat tctgacaaaa actaccaaag
     5401 ataccttgaa actctggaag tggtaaaaga aggactcagt tcagaaactc taacgtacca
     5461 aggtcaattt caccaatatg acaacctccc aatgcgatta gaaccgattc aaaaaccgtt
     5521 cccaccaatg tggtatatga gaaacgccga aacagccgcc ttggaaggaa tgaacactgt
     5581 gattgtagga actctggaaa gcctcaaggc tgaaaccaag agatataaag atgtctggaa
     5641 taagactcac ggtgaaggct cactaaacga tcagggtcaa gatccaaaaa ttggtcttgt
     5701 tgtccatatg gttgtagctc gtacagacga agaagccata aatattgcga ctccagcctg
     5761 ggaaaaatat aaatggaatt tagcagctcc tcggcgaata gaagctgaaa aaagagggtt
     5821 ggaacagttc caatcttcaa aggatggctc attcgggttc gtgggagaca gaccggctag
     5881 tcttccaact agggaaacca gaaaggatat agaggctgaa atagagcgat tcgaccagca
     5941 atcaaacaga aaggatccta ctagactagg gggtgtcgct ttggcaggtt ccccaaagtc
     6001 agtggaacgt tatttggaag aatacatcga aaccggtgcc aattattttg tgtgttcgtt
     6061 tcaatgggga gatattgacc acgaaactgc tatgaactcg attaatcttt tttcagaaca
     6121 tatactgccg caatatattt agataatttt ggatagattc atgaaacttg agataattgg
     6181 aatgatcggc gcagtaaggg aaggtactgg aagtaaaggt gcagaggtac acatcattgg
     6241 tggcggggta gacggggatt atatctccga ttttgctaaa gcccatgaga acagcggttt
     6301 cgataaagtt ctggtcggat acacatcttc atctgcagat gggttcatag tcgcgatgca
     6361 tgcagcagcc aacactgaag aacttggatt tttggttgct caccggcccg gatttgtcag
     6421 tccgacagta ctaagtagga aggccgccac tatcgatcaa ttaacacaag gaagaattgc
     6481 tctacacata ataagcggag gtggggactt agaacagagg cgtgatggcg attttctaga
     6541 ccatgatgcc aggtatagaa ggtcaggaga attcatgcac attcttaaaa ccctatggac
     6601 ttcaaaaacg cccgtcgacc ataagggtga gttctatcaa ttcgaacaag cgaaaagtga
     6661 ctttgaatgc tttcagaaac cacatatccc tctgtatttt ggtggagctt ctgatgcagc
     6721 attagaagtg ggtgcgagag aatgcaacgt atatgctatg tggggagaac cgctcaaaga
     6781 agttgaaaaa aaaatcagat tattcacaga cagagtaaca agagttggaa gggatccaaa
     6841 atctgtaaga ttttccgttt caacaagacc gatagtggct gatactgaat ctgaagcctg
     6901 ggatattgct cattccactc tagaaaatgt tctgaaaaca aaccagaatt ctatgcatgg
     6961 tatgggaaag gaaaggttgg aatctgtagg atctcagcgt ctcatagatt tcgcaaaaga
     7021 tggggaaatc ttggatgatc ggctttacac tcccatagcg gcagctactc gaggtgcagg
     7081 aaatactaca gcacttgtag gcaccgcagg acaagtatct gattcactta tgaaatacta
     7141 caaacttggt tgtagcactt tattagtaag aggttttgat gcatataatg acgctgttta
     7201 ttggggtaaa gaactaatcc caaatctaaa agaatcggtt gctaattatc ataaaaggca
     7261 cgcagttact ggaaaacact tactatgaaa atattatcgg cggttctagg aagtccattc
     7321 caaggttcat cttctgaaaa aatcgtaaat atttttattg ataggctccc taaagaacgg
     7381 tgggacacag cactggtgga tttgtctaaa atttcgtcag aagctctttt actaaggagt
     7441 aaagataaag cattaaatgc ttctatagaa cgtgtcctta attccgatgt gatcatcgct
     7501 gccaccccga catatagagc cacctataca ggtctaataa aaactttttt tgatcaattt
     7561 ccagaaaatt ccctgtctgg caaattagtg ctacccatcc aaaccggagg atctgcggaa
     7621 cacgctttgt cagttgaaca tggtttgact ccaatggtca ggacattagg agccgtcgtc
     7681 gccaacaagt cgatttactc ttggggagag cattggaatg aagatggaaa tccaggtcaa
     7741 aatttaaagg aaatgattga tcaatcagtg aatgaaagta tcaatacgtc tggttaatca
     7801 tccaacaatc ccaaagcacg agccatatca agcatctcca aagctgtttt ctcatgagca
     7861 atatcaacca ttccgccgtg aaaagaaacg gcggcccccc c
//
LOCUS       DASCNS010000233         4194 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001816325, whole genome shotgun sequence.
ACCESSION   DASCNS010000233 DASCNS010000000
VERSION     DASCNS010000233.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001816325"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(231..986)
                     /locus_tag="QF838_08470"
     CDS             complement(231..986)
                     /locus_tag="QF838_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60751.1"
                     /translation="MRLENKVALLTGIGQGMGRATARLFAQEGAKTALSARGEERLKE
                     TASQIESLGGTAIVVPGDLSEKQAVEEIVNNVVDEFGKIDILYAAAGGNFDPNRSLED
                     IDQSFWDSTAANTMNSLYNLAQAVRPVMKESGAGSIVTVAASFNVRQAGNASYGAAKS
                     AVIGMSQNLAGELYADNIRVNTIAAGLFRGTIGDGRVVNAEVNLSRTGYPQDIANAAL
                     FFASDESAWITGQVLAVDGGVDVGTRGLWDHER"
     gene            complement(1086..2204)
                     /locus_tag="QF838_08475"
     CDS             complement(1086..2204)
                     /locus_tag="QF838_08475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJO60752.1"
                     /translation="MRNKKIAIIGAGIVGASVAFHLSQHGIDVSVFDKNQPGTGASGH
                     SFAWINAFSKQPRHYYDLNYRSMDRWPRFSENLGEDIGLRWGGNVTYCSDPDEGRRLA
                     SECERLNSWGYSARLISMEELSRFEPNLKIGQFHTGIYTPDEGHVLPVKVAEACLHKI
                     QEAGGKIYLDTPVDSITQSADKVFLRLDEQEMEFDQVVIAAGIDSTKLAKTAGINLPQ
                     RRSPGVVVKTKPLPSFIHSLATIYLPRISKEEKEIHIRQDIDGAVMIGAGDQESETED
                     DSQEYANKLIQRAARYFDQLSGVEAIRVPVGLRPMPEDDLPVIGFSPGHNNIYLTLMH
                     SGVTLAPLVGALAALEMVTGIPNGDLDPYRPDRFASKN"
     gene            complement(2296..2616)
                     /locus_tag="QF838_08480"
     CDS             complement(2296..2616)
                     /locus_tag="QF838_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60753.1"
                     /translation="MFHIKRVFKAKPGQSRKVATLAYQAAETYSGAGQRGEFSVYFNP
                     GTTPGEKDIVVLQWADEALKSVFREENEIPSKSLEIWRELLTLTEENWIEFNELLTPD
                     KMVK"
     gene            complement(2846..>4194)
                     /locus_tag="QF838_08485"
     CDS             complement(2846..>4194)
                     /locus_tag="QF838_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60754.1"
                     /translation="IDQRFDRLRYRNLYHKNGSYNLLAPYRPSREDVIDSFGLGTEWD
                     IAGNGQHYFRNITPIFVWGVGGPDTGPNSVIGMASPPVWSQDTELDISHEEAHRTWGH
                     ELGHAYFGLPDYYHRGAPAGPNKTGPLDIMGDRLGSNEGKQWPPLTAWSLIKSEIQEP
                     EQLFTDTDLLAVLGNASGLIEGPGSLYSRENENGANIIKIPAIIERDTQLVKGYYLLE
                     LYGSTGYDGQIYVNSNTVNFADNAGAFPGGIAIWKWDTTEQKIRERCATWSSCTGYEF
                     LYNNGNITDFIEYYPSIPSITPGNDADWPGSSPGIYNLFPWWYSAQLPYGADYENDLS
                     NMPTTIELPVLEIAPAAEDPNYGGGNKVATITLSFDEFIDDIKTKVRDDSQRANNGNL
                     NGYTFGDTAAFTYSVEKHAAPEYMTYKDHVREGQIQFASGEMWDEMQAYGFFRFTE"
BASE COUNT         1079 a         1035 c          828 g         1252 t
ORIGIN      
        1 attgttttca ctaaaaagcg ggcgttatag accgtcattt tctacgttcc ttaactggca
       61 accatgggcc tggaggccct tgggaaggtt tacaaacgag gcaggctcta aaaccttcct
      121 cataagcctg ttttaaggat cggaaatatc gtctattttg agatttggta cgtctacctg
      181 gagggcagtt aggcctgcat acaatttttg tagtagtaca agcgttaaat ttagcgttca
      241 tggtcccata gacctcgggt acctacatcc acgccgccat ccacagccaa gacctgccca
      301 gttatccaag ctgattcatc tgatgcaaaa aacaacgctg cattggctat atcttgagga
      361 tagccggtac ggctcaaatt gacctcggca tttaccactc ttccatctcc aatagtcccc
      421 cgaaacaatc cagcagctat agtatttact ctaatattgt ctgcatatag ttcgcccgca
      481 agattttgtg acattccaat aacggcactt ttggcagcac cgtatgaagc atttcctgcc
      541 tgacgcacat tgaagctagc tgcgacggtt actatcgatc cggcaccact ctctttcata
      601 acagggcgga cggcctgagc aaggttatat aaactattca ttgtgttggc ggccgtactg
      661 tcccaaaagc tttgatcgat atcctctaga ctgcggttcg gatcaaaatt acctccagct
      721 gcagcatata gaatgtctat tttcccaaac tcatcgacta cgttgttcac aatttcctca
      781 accgcctgtt tttcagaaag atctccagga actacgatcg cggttccccc cagactctct
      841 atttgacttg ctgtctcttt aagacgttct tcccctcttg ccgagagagc tgttttggct
      901 ccttcttggg caaacaatcg tgctgttgct ctgcccattc cttgtccaat accagttagc
      961 aaagcaacct tattttctaa tctcatattt ctcactattt aactacttag ttattaagct
     1021 aattggattt gacgcccccg aactatagat attggaggag aaatccacaa ctctaattca
     1081 aacgatcaat tcttggacgc aaatctgtcc ggacgatatg ggtctaaatc accattgggt
     1141 attcccgtta ccatttccaa tgccgccaaa gcacccacca gtggggcaag tgtgacacca
     1201 ctatgcataa gggtaagata gatattatta tgcccgggtg aaaaccctat taccggaaga
     1261 tcgtcctctg gcataggcct aagacccaca ggaaccctta ttgcctcaac acctgatagt
     1321 tggtcaaaat atcgagctgc cctttgaatg agcttattcg catattcttg tgaatcatct
     1381 tccgtctcac tttcctggtc accggcaccg atcatcaccg cgccatcaat atcttgcctg
     1441 atatgtattt ctttttcttc tttgctgatc cgtggcagat atatggtggc caaactatgg
     1501 atgaaggagg gcaggggttt tgttttcaca accaccccgg gacttcttct ctgaggcagg
     1561 tttatacctg cggtcttcgc caatttagtc gagtctattc ccgcagctat cactacttgg
     1621 tcaaattcca tttcctgctc atccaggcgc agaaaaactt tgtcagcgct ttgggtaatg
     1681 gaatcgacag gggtgtccaa atagattttc ccaccagctt cctgaatctt atgcaaacag
     1741 gcttccgcca ccttgacagg taaaacgtgt ccttcgtcgg gggtgtaaat accagtatga
     1801 aattgcccga ttttcaagtt cggttcaaac ctggaaagtt cctccattga tattagtctc
     1861 gctgaatatc cccatgagtt aagtcgttca cactctgaag ccaatcttcg accttcatca
     1921 ggatccgaac agtaggtgac gttcccgccc caacggagac caatgtcttc acccaaattt
     1981 tcagaaaatc taggccaacg atccatggaa cgatagttta aatcataata atgccgaggt
     2041 tgtttcgaga aggcattgat ccaagcgaaa gagtgaccgc ttgcaccagt tccaggctga
     2101 tttttatcga atacagacac gtcgatccca tgttgggaca agtggaaagc caccgatgcg
     2161 cctacgatcc ccgcaccaat tattgcgatt tttttgtttc tcatgattta agttttaaaa
     2221 atatgttttt ttcactcaac cggacaataa ctcgacactt gattttcaca tcaagtcatc
     2281 ttaagaaaaa ctgattcatt taaccatttt atccggtgtc agaagttcat taaattcaat
     2341 ccaattttct tccgtaagcg tcagtaactc tctccaaatt tcgagtgact tagatggaat
     2401 ttcgttctct tctcgaaaaa ctgatttcaa ggcttcatct gcccattgca aaaccacgat
     2461 atctttttcc cccggggtag taccaggatt aaaataaact gaaaactctc ctcgctgtcc
     2521 ggcaccgctg taggtttctg ctgcttgata ggctagagtg gcgactttac gagactgacc
     2581 tggcttagcc ttaaatactc ttttaatatg aaacattctt cctccttagt gagacaatat
     2641 tattgatgat atcactaggt atcaggaatg aaacagtatc agcataaaat cgggtaacca
     2701 caaaaatctt tgcgcagtat ctgctcgagc accaccctgg gctactcgaa ttacttacag
     2761 taaccacctc agagttataa gtcctctgag acggttattg caaaatagtt aagccgattc
     2821 gttgcaatcg ctattcttct gtggcttatt cagtgaacct aaagaaacca taggcctgca
     2881 tctcatccca catctcacct gaggcgaatt ggatttgacc ttccctcaca tggtctttat
     2941 aggtcatgta ttctggggct gcatgtttct caacactata tgtaaatgcg gcagtatcac
     3001 cgaaggtata cccattcaaa ttaccattat tggccctctg tgaatcatcc cgtacctttg
     3061 tttttatatc atctataaat tcatcaaatg agagagttat ggtagcgact ttgttaccgc
     3121 caccgtaatt cggatcttca gcagccggtg caatttccaa taccggcaac tctattgtgg
     3181 ttggcatatt agacagatcg ttttcatagt cagccccgta tggtaactgc gctgagtacc
     3241 accatgggaa caagttgtat atcccgggag agctgcccgg ccagtctgca tcatttccag
     3301 gtgtgatact tgggatactg gggtagtact ctataaagtc tgtaatgttt ccattattgt
     3361 ataagaattc gtaaccagta cacgaagacc aggttgcaca tcgttctcta attttttgct
     3421 ctgttgtatc ccatttccat atggctattc cacccgggaa ggcaccagcg ttatcagcaa
     3481 aatttactgt gtttgaattc acatatattt gcccgtcgta tccagtagac ccatatagct
     3541 ctagtaagta ataccctttc actaattgag tatctcgctc tattattgca ggaattttaa
     3601 ttatgttagc tccgttttca ttttccctac tgtaaaggct tccaggacct tcgattagac
     3661 cagatgcatt accaaggaca gcgagtaaat cggtatcggt gaataattgc tctggctctt
     3721 ggatttctga tttgatcaag ctccaagccg tcaatggtgg ccactgtttt ccctcattgc
     3781 tgcctaaacg atcgcccatg atatccaaag gtcctgtctt attcggtcct gcaggagcac
     3841 ccctgtggta gtagtctggc agtccaaaat acgcgtggcc taattcatgg ccccatgttc
     3901 tgtgggcttc ttcatgtgat atatcaagct cggtgtcttg ggaccatacg ggtgggctgg
     3961 ccatacctat tacgctatta gggccagtat ccggacctcc gaccccccag acgaaaatag
     4021 gagttatatt tctaaagtaa tgctggccat ttccggctat atcccattcg gttccaagac
     4081 cgaatgaatc gattacatct tctcggctcg gtcggtatgg ggcaagcaaa ttatatgacc
     4141 cgtttttatg gtacaaattt ctgtacctta atcggtcaaa acgttgatct attt
//
LOCUS       DASCNS010000234         4888 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001829705, whole genome shotgun sequence.
ACCESSION   DASCNS010000234 DASCNS010000000
VERSION     DASCNS010000234.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001829705"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="QF838_08490"
     CDS             complement(<1..829)
                     /locus_tag="QF838_08490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HJO60755.1"
                     /translation="MATIRTEPVTINLGPQHPSTHGVFRLRVTFDGEEIVDLEPVFGY
                     LHRGTEKLAETRNYVQIVTLTDRMDYVASMSNNLAYVRTVEKLSDIEVPERAQYIRVI
                     SVELQRIASHLMATGFLLNDLGALATPLVYCFRERERILDLFEMLCGARITLSYMRPG
                     GVFQDAPEEFWSSLNTLISDFPNYLDELESLITTNEIVLARTIGVGLLSDAQAVDSGV
                     TGPILRASSVKWDLRKANPYEIYDRVNFDIPIGSSGDTYDRYYVRLQEMRESIKIISQ
                     "
     gene            complement(834..1277)
                     /locus_tag="QF838_08495"
     CDS             complement(834..1277)
                     /locus_tag="QF838_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HJO60756.1"
                     /translation="MTKIITGEEISKSIESTLANAVVASDETAMWVSPETIHEVCETL
                     KDPDGLDYSFLTAISAVDYIEYFEIVYHLTSLRNNCSCIIKVKTSGRENPEVQSVSDI
                     WQAADLQEREIWDLMGIKFEGHPNLKRILLWEGFNGHPLRKDFIG"
     gene            complement(1280..1897)
                     /locus_tag="QF838_08500"
     CDS             complement(1280..1897)
                     /locus_tag="QF838_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HJO60757.1"
                     /translation="MINTNNPTQPLLIPLHDKTWDLDREEGSYVNELKATHTEPLSEP
                     LLEVPDDLGRNVAVTSVDALLNWGRKSAVWPLSFGLACCAFEMMASAMSRFDLSRFGM
                     EVFRPSPRQADLMIIAGTVTWKMAPAIERVYEQMADPKWVISMGVCATSGGPYYGSYS
                     VVPGVNHIIPVDVYVPGCPPRPDALLYGIMQLHEKIKRYSIKNRG"
     gene            complement(1906..2301)
                     /gene="ndhC"
                     /locus_tag="QF838_08505"
     CDS             complement(1906..2301)
                     /gene="ndhC"
                     /locus_tag="QF838_08505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HJO60758.1"
                     /translation="MADEYFRQYGVIAIFAALAVAVPTSMLLIGWGLSLLNVRPKKPS
                     FVKNSIYECGFETITERWSQFNFRYYSIALLFVIFDVEVVFLFPWAASFGTMSQEFGL
                     FVLGEMTVFIGILVLGWIYAWKKGSLEWS"
     gene            2595..3638
                     /locus_tag="QF838_08510"
     CDS             2595..3638
                     /locus_tag="QF838_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012568.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HJO60759.1"
                     /translation="MPDSVHLKPDLPSVLSRYRDVLDLALKDTVSDRHVFLYDMLRYC
                     MGWSDADGTPLETEKGKGVRPSLCLFTCDALGGDIEKALPAAVSLELIHNFSLIHDEI
                     QDSDEIRHHRPTLWNIWGVPKALFAGNVMRVLADRSMFTMLSDDPKLLSASSSIVSEA
                     CLEMIEGQYMDISFEGGNSITVDQYMKMISHKTGALLRSSVHIGAIIGSRDGRDVDIF
                     REIGVKLGFMFQIRDDILGTWGETTSTGKPVGSDIRRKKNTLPIIHARETQTQMYKKE
                     ISELYSLESLEDHHVDRLLDILDATKASHYCQDMVENYALEVIKDIQNIRFSNNSRGD
                     FRELVSFLVDRSF"
     gene            3742..4224
                     /locus_tag="QF838_08515"
     CDS             3742..4224
                     /locus_tag="QF838_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HJO60760.1"
                     /translation="MSVSHATMKTLTYYLRVPSSSSPEFIDITDEVNGFLKDSGISSG
                     MLLVFSRHTTSAIVIQENEPLLLEDFKTLLENTASKDASYRHNDFDVRTVHMHENECP
                     NGHSHCQHLVLGSSEMVPVIDGDMPLGEWQRIFMVELDGMKGEMVGSREIVVQIMGLG
                     "
     gene            4444..>4888
                     /locus_tag="QF838_08520"
     CDS             4444..>4888
                     /locus_tag="QF838_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HJO60761.1"
                     /translation="MADQKVISRQTGVEDTQYKWGFEFDIESNVAPKGLNEGTVRFIS
                     SKKKEPEWLLEWRLKAFEHWGKQGEEASPKWAKLKFEDIDFQDIIYYAAPKSSDDAPK
                     SLDEVDPEILEAFDKLGIPLDEQKKLSGVAVDAVLDSVSVATTYQD"
BASE COUNT         1409 a         1003 c         1050 g         1426 t
ORIGIN      
        1 actgtgaaat aatttttatg ctttctcgca tttcttgaag acgaacataa taccggtcgt
       61 aggtgtctcc agagcttcca atcggtatgt caaaattcac tctgtcatat atttcgtaag
      121 ggtttgcttt tcttaaatcc catttgacac ttgaagccct gagtatcgga cccgttactc
      181 ctgaatctac tgcttgggca tcggaaagca aacctacccc tatcgtcctc gctagaacga
      241 tttcgttggt tgttattagg ctttccaatt catcaaggta atttgggaaa tctgatatta
      301 aggtgttcaa tgaactccaa aactcttcag gagcatcttg gaagaccccg cctggtctca
      361 tgtagcttaa ggtgattctt gcgccgcaca gcatttcgaa cagatctaga attctttctc
      421 tttcgcgaaa acaataaact agtggggttg caagggctcc taagtcgttt aacaggaagc
      481 ccgtggccat aagatggctg gcaattcttt gtagttcgac acttatgacc cgtatatatt
      541 gggccctttc tgggacctct atatcagata atttttcgac agtccttaca taggctaaat
      601 tgttcgacat actcgctacg tagtccatcc gatctgtcag ggtcactatt tggacatagt
      661 ttctggtttc agccaatttt tcggttcccc gatgaaggta accgaatact ggctctagat
      721 ccactatttc ttcgccgtca aaagtaacac gcaacctgaa cacaccgtgg gtactagggt
      781 gctgaggacc tagatttata gttactggtt ctgttctaat ggtcgccata aaatcagcct
      841 atgaagtcct ttcgaagggg atgaccgttg aaaccttccc aaagtaatat cctctttagg
      901 tttggatgac cctcaaattt gattcccatt aaatcccata tttctctttc ttgtaggtca
      961 gccgcctgcc aaatatctga tactgactgt acttcagggt tttccctgcc gctggtcttt
     1021 actttgatga tgcatgagca attgtttctg agagatgtca aatgatagac aatttcaaag
     1081 tattcaatgt aatcaactgc gcttatagca gtgaggaagg aatagtccag tccatcaggg
     1141 tctttgaggg tttcacatac ctcatgtatg gtttctggcg aaacccacat agcagtttca
     1201 tccgaagcaa caactgcgtt agcgagtgtg ctttctatgg attttgaaat ttcttccccg
     1261 gtgataatct tagtcaagat caccctctgt tttttatgga atatcgtttg attttttcgt
     1321 gtaactgcat aattccgtat agaagggcat ccggccgagg tgggcaacct ggcacgtaaa
     1381 catcaacagg aataatatga tttaccccgg gaacaacgct gtatgaacca taatatggcc
     1441 ccccactggt agcgcacaca cccatagaaa taacccattt aggatcagcc atctgttcgt
     1501 aaactctctc aattgcggga gccattttcc aggttacagt acctgcaatg atcataagat
     1561 ctgcttgtcg gggcgatggc ctgaaaactt ccatgccgaa cctagaaaga tcaaatctag
     1621 acatggcact tgccatcatc tcaaaggcgc agcaagcaag gccaaaggat agtggccaaa
     1681 cggcagattt gcggccccaa tttaacaatg cgtcaacgga ggttactgcc acatttcggc
     1741 ctaaatcatc cggaacttcc agcaagggct cagacaaagg ttctgtatga gtggctttca
     1801 gttcattgac ataactacct tcctctctat ctaaatccca agtcttatca tgtaaaggaa
     1861 ttaggagagg ttgggttgga ttgttcgtgt ttatcatgta tccattcaac tccactcaag
     1921 tgaacctttt ttccatgcat agatccagcc taacaccagt atgccgatga aaactgtcat
     1981 ttctccaaga acaaataaac caaattcttg ggacattgtc ccgaaagagg ctgcccatgg
     2041 gaataaaaaa accacctcga catcaaaaat tacaaatagc aaggctatgc tgtaatatcg
     2101 aaaattaaat tggctccatc gttcggtaat ggtctcaaac ccacattcgt atatgctatt
     2161 tttcacaaat gatggtttct taggtctcac atttaggagg gatagtcccc agccaattag
     2221 aagcatacta gtgggcacag caacagccaa tgcggcaaat atggcgatga caccgtattg
     2281 tctgaaatat tcgtcagcca tcattcacac cattatctct gaggattggt cgtagttgtg
     2341 atacgttaaa gagttcctac ctgatcgcta cagctccatg cgaaaatata gcacacagct
     2401 cttttcagta gcaagtgaaa aaacgcacaa acgaatgggt atagaggact atattggata
     2461 atatttaggg gttaatttca ataaactaac cgatccgtaa aagtatatta atattgcgtg
     2521 tcactttatg aatctctact gaaaccaata caatggggat aggtttgcaa aaattaataa
     2581 tttgagtgta aaaattgcct gatagtgttc atctgaaacc agatcttccc tcagttctta
     2641 gtaggtatag agacgttttg gaccttgcac taaaagatac tgtttcggac cgccatgttt
     2701 tcttgtatga catgctgaga tattgcatgg gctggtcgga cgctgatggt acacctctcg
     2761 agactgaaaa agggaaaggc gttcgacctt ctttatgtct ctttacttgt gatgccttag
     2821 ggggtgacat agagaaagca ctcccagcag cggtatcact tgagctaata cataattttt
     2881 ctcttataca cgatgaaata caggactctg atgaaataag acatcatcgc cctactcttt
     2941 ggaacatatg gggtgttcct aaggccttat tcgccgggaa tgtgatgaga gtgttggctg
     3001 acagatctat gtttactatg ctgtcagatg atccgaaact gctttctgcc tcgtcttcaa
     3061 ttgtgtcaga ggcttgtttg gaaatgattg aaggtcagta catggacata tcttttgagg
     3121 gtgggaatag tataaccgtg gatcagtata tgaaaatgat ttctcataaa acgggagcac
     3181 tactcaggtc ctcagttcac ataggggcca ttattggttc gagggatgga agagacgtag
     3241 atatttttcg agaaattggc gtcaaactag gatttatgtt tcaaattagg gatgatattc
     3301 tcggtacttg gggtgagact acatctactg gaaagcctgt tggttctgac atacgtagaa
     3361 agaaaaacac tcttcctatt atccatgctc gagaaactca aacccaaatg tataaaaaag
     3421 agatatctga gctgtactct ctggaatcac tagaagatca ccatgtggac cgccttttgg
     3481 atatcttaga tgctactaaa gccagccatt attgccagga catggttgaa aattatgcat
     3541 tagaagtcat caaagacata caaaatataa ggttcagcaa caactctagg ggggacttca
     3601 gagaacttgt atcttttctt gtagacagaa gcttttagag tagaatctac ctgtgagtga
     3661 agtttcttga tatctattat aatattttct ccaaactaga ataaccaatt tgaatcggca
     3721 cactgcgagg tgggtagata tatgagcgtt tcccacgcca cgatgaaaac cttaacttat
     3781 tatctcagag taccctctag ttcttcgcct gagtttattg atataacaga tgaagtaaat
     3841 ggatttctaa aggattctgg gatttccagt ggcatgctcc tagtattttc acgtcacact
     3901 acctcagcca ttgtcataca ggagaatgaa ccgcttttgc tcgaagattt caaaacactg
     3961 ttagaaaaca ccgcttctaa agatgcttct tataggcata atgatttcga tgtgagaaca
     4021 gtacacatgc atgagaatga atgtcccaat ggtcattccc attgccagca cctggtactc
     4081 ggctctagcg aaatggtacc tgtgatagat ggagatatgc cattaggtga gtggcaacga
     4141 atatttatgg tcgaacttga tgggatgaaa ggtgaaatgg ttggtagtag agagattgtt
     4201 gtccaaataa tgggcttggg ataaaaccta tagaaaccca aatcttttcc tcacggctaa
     4261 tttcccacct gaactgtcag taactttttt gacctccggt tctcccagat gttatatttg
     4321 ttcggttatt gttgactaat taactcaaca aatttttacc gtgcgagtct caaatattta
     4381 ttgggcttgt ggattgtatt gctgtttatc aaaagaggga atcactgtaa ggagaagtct
     4441 tgaatggctg accaaaaggt tatatccaga cagacaggag ttgaggatac ccaatacaaa
     4501 tggggatttg aatttgatat agaatccaac gtggccccta agggcctaaa tgaaggcacc
     4561 gttcggttta tatcttcaaa gaaaaaggag ccagagtggt tgctggaatg gcgcttgaag
     4621 gcatttgagc actggggaaa acaaggagag gaggcctctc caaagtgggc gaagctcaaa
     4681 tttgaggata ttgattttca agacattatt tactatgcgg cacctaagtc cagcgatgat
     4741 gcccctaaaa gccttgatga ggtggacccc gaaatactcg aggcgtttga taagttaggg
     4801 attccattag atgaacagaa aaaattatca ggcgtagctg tagatgcggt tttggacagt
     4861 gtttctgtag caactactta tcaggaca
//
LOCUS       DASCNS010000235         7828 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001834832, whole genome shotgun sequence.
ACCESSION   DASCNS010000235 DASCNS010000000
VERSION     DASCNS010000235.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001834832"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="atpA"
                     /locus_tag="QF838_08525"
     CDS             complement(<1..1006)
                     /gene="atpA"
                     /locus_tag="QF838_08525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HJO60762.1"
                     /translation="MAVRGQDIASVIKKQIEGFGTDLGMVDVGTVIEVGDGIARVHGL
                     SGVAYNELVEFEGGVAGMALNLEEDSVGIVILGDPSGISEGTEVRGTGRVMQVPVGEG
                     LLGRVVDGIGNAIDGKGPVDTNETREVEIVAPNVAIRKSVDTPVQTGIKAVDAMIPIG
                     RGQRELIIGDRTTGKTAICTDAIINQKGGDMVCVYVAVGQKVGKVAQIVGILEQQGAM
                     EHTIVVAANASDSAPAQYLAPYTGCAMAEYFMAQGKDVLIAYDDLSKHAWAYRQMSLL
                     LKRPPGREAYPGDVFYLHSRLLERAAKMDDAHGGGSITALPVIETQAGDVSAYVPTNV
                     I"
     gene            complement(1069..1602)
                     /gene="atpH"
                     /locus_tag="QF838_08530"
     CDS             complement(1069..1602)
                     /gene="atpH"
                     /locus_tag="QF838_08530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HJO60763.1"
                     /translation="MALSARRYAQALFLIAEEKGDQEQWLADLELLADSSRNSDFIAF
                     IDSPKIENVKKIEVIKEAFSKSVSEMALNFVSLLATRNSVASLPNIADAFQELVDSEK
                     GVERAEIVSAVKLTDAQERDIVGKLARMVGKELSVTTYVDESILGGYLARVGDRLVDG
                     SVKTQLENMRRELIRGA"
     gene            complement(1605..2102)
                     /gene="atpF"
                     /locus_tag="QF838_08535"
     CDS             complement(1605..2102)
                     /gene="atpF"
                     /locus_tag="QF838_08535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HJO60764.1"
                     /translation="MGDLGINLPGLITQIISFVILFVILSKVLYKPLVAMLDQRAEKI
                     TAGLEAAEKAKEEAAKSEEAIQEQLQEARSEGQKLVAQARETADRFREEEMAKVREDL
                     DAERSRAESNIQRERDAAIEELRKEFASLAITAAEKVVKTSLDEEGHRTLIEGVLNEE
                     GSRNN"
     gene            complement(2180..2404)
                     /gene="atpE"
                     /locus_tag="QF838_08540"
     CDS             complement(2180..2404)
                     /gene="atpE"
                     /locus_tag="QF838_08540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HJO60765.1"
                     /translation="MEGDIMNVGAGLAIGLGAIGPGIGIGMLASKAMEALGRNPEAGA
                     QIQQNMILAIAFTEAIAIYALVVAIIIKFV"
     gene            complement(2466..3629)
                     /locus_tag="QF838_08545"
     CDS             complement(2466..3629)
                     /locus_tag="QF838_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012347.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HJO60766.1"
                     /translation="MSKILGNPRSLAILIICVAVLAVSILGGALGNTLGFGFFASSLP
                     AIQLPAEKVTDGFNTPIGKWDLMNTMVTTWLAMLVLIVISLVIRKNLKDVPSRLQALF
                     ELIIEFFLALCEQIAGKEKARRFFPLVFTIFIFIVAANWMGILPGFGTVGRIAGPEEI
                     AHHHNAHDYHDPALMQTTLESKESKAKSHASGETHGDDHDSHLGAVKLHVFRDYKSVP
                     LATLSPGSLEEELTFSQYGENHGNVGEGKRAGHLVPFLRSANTDLNMSLAIALIAMVM
                     VHFWGFSILGFFGHAGKFISFKSGPIGFFVGILELISELGRVISFTFRLFGNIFAGEV
                     LLMAMAFLLPLIGIIPFLGLELFVGLIQAFVFAMLTLVFASMASISHDDGDHH"
     gene            complement(3648..3872)
                     /locus_tag="QF838_08550"
     CDS             complement(3648..3872)
                     /locus_tag="QF838_08550"
                     /inference="COORDINATES: protein motif:HMM:NF021073.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HJO60767.1"
                     /translation="MNTKKYGAIVRLVGVGWYVGICIGVGAWAGLWADNRFGLSPILT
                     LAGITIGLVVALTGMIRMLMAVLRYVSESD"
     gene            complement(3947..4477)
                     /locus_tag="QF838_08555"
     CDS             complement(3947..4477)
                     /locus_tag="QF838_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HJO60768.1"
                     /translation="MLELEIESIRVRQETQQRAVVLKVKDSDLYLPIFIGQFEVEAIR
                     FKLMEVEVQRPMTHDLLGSVIGDLGGTIHSIVVSELKDDTFFAKIVIDTNGTLIEIDA
                     RPSDALALAVRSEAPIYAEDEVVEKAGVSLDVEPESADTKGSEEKQVNEEELEGLSAF
                     TDFIDSLDLEDIGNKG"
     gene            complement(4588..4776)
                     /locus_tag="QF838_08560"
     CDS             complement(4588..4776)
                     /locus_tag="QF838_08560"
                     /inference="COORDINATES: protein motif:HMM:NF017879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HJO60769.1"
                     /translation="MVTDYSLGDIITLKKKHPCGEVIWRVDRIGADIGIVCRGCNRRL
                     IIPRSDLEKRTRSHRKTE"
     gene            complement(4800..5636)
                     /gene="pyrF"
                     /locus_tag="QF838_08565"
     CDS             complement(4800..5636)
                     /gene="pyrF"
                     /locus_tag="QF838_08565"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HJO60770.1"
                     /translation="MTKFADSLQDISVSKKSLLTIGLDPDPVKMPVSDVFEFCKLIID
                     HTEGFTAAYKPNLGFFEAFGLEGLKSLEKVIDHIKSNYSDVLIIGDAKRGDIAPTSSK
                     YAYAMFEIWDFDAVTVNAFSGLDSIEPFLRYTDRGVFVWCKSSNPDGYQLQDLVVKGT
                     TDRKIYQVIAEYCVSWNNEGNLGLVVGATYPDELKIVRDIVGELPILVPGIGSQSGDL
                     EASVRVGLGRENHGLLVSSSRGIIYASSDRNNFGSAAANTAANLRENINNILEDLGRS
                     FI"
     gene            complement(5668..6150)
                     /locus_tag="QF838_08570"
     CDS             complement(5668..6150)
                     /locus_tag="QF838_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJO60771.1"
                     /translation="MTSKKIIVDSRDSFGGWFEEFSPGQIFKHWPGKTITEMDNHLFS
                     LLTMNDNPLHTDENYMSEHQHGKTLVNGLLVMSLVVGMSVRQTSGKAIANLLYESVTH
                     DGPTFHGDTIYAESEVLTVTASQSRNDRGLVYIESRGINQKKEKILTLRRKFLVQKRS
                     "
     gene            complement(6169..7326)
                     /locus_tag="QF838_08575"
     CDS             complement(6169..7326)
                     /locus_tag="QF838_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60772.1"
                     /translation="MTQTSEEARKQIVSLVRDFVKRDVEPIANSYDNEDIYPHELIPT
                     MCELGLFGINIPTEYGGMGLDFTTFAMIFEELSKGWMSVSGIIGTHHILSYIITIHGT
                     EEQKHRILPRMATGEIRGGLALTEAEAGSDAQNISTTATKDGGEYVINGRKMFISNGD
                     NGNAFALMAKTDPNSNPRHRGISCFIFEKPTEGFKVGQHLDKLGYRGLDTCELIFDDC
                     RVPVDNLIGGEEGRGFGQVMNGLETGRINVAARAVGVAQAALEASVSYSKKRSAFGKL
                     ISEHQAIQLKLAEMAAKVHAARLMVYDAASKKDSGERNDLETAMAKLFASEVCGEVAM
                     EAMRVHGGVGYTKDLPVERYYRDAPLMIIGEGTNEIMKLVIARRLLEKYSD"
     gene            complement(7351..>7828)
                     /locus_tag="QF838_08580"
     CDS             complement(7351..>7828)
                     /locus_tag="QF838_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012966.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HJO60773.1"
                     /translation="GRNGVGIGTHVIENRFVGIKSRGIYESPGMELLGQSWEYLLQFV
                     LDRRARDFYTNLSNLISTQIYQGYWLDSATSSALAALSPLASMVTGSIQIKLYKGNIF
                     FDSVDDSIEKIPFSLYTDDGSMEAMGGYDHKDAEGFLNVLGVSAKNVGNRQYRDSI"
BASE COUNT         2159 a         2007 c         1313 g         2349 t
ORIGIN      
        1 atataacatt agtggggaca taagctgata cgtctccagc ttgagtttca attactggca
       61 aagccgttat cgacccacca ccatgagcat catccatttt tgcagccctc tctaacaacc
      121 tgctatgaag ataaaaaaca tctccaggat aggcttctcg gcctggtggc ctcttcagca
      181 gtaacgacat ctgtctgtaa gcccaagcgt gtttggacaa atcgtcatag gctatcagga
      241 catccttacc ttgagccata aaatattcag ccattgcaca tcctgtgtac ggagctaaat
      301 attgagccgg agctgaatca gatgcgttgg ctgcgacaac aatggtgtgt tccattgcgc
      361 cttgttgttc taggattccg actatctggg cgaccttacc aactttctga ccaacggcaa
      421 catagacaca aaccatatcc ccacccttct ggttaatgat ggcgtcagta catatagctg
      481 ttttacctgt tgtacggtct ccaataatta actctctttg cccacgtccg attggtatca
      541 ttgcatccac agccttaatc cctgtctgca caggggtatc aacggacttc cttattgcca
      601 cattgggggc gacaatttcc acttccctag tctcattggt atccactgga ccttttccat
      661 caatggcgtt accaatccca tcgacaactc tacctagaag tccttccccg actggaacct
      721 gcattactct tccagttcct ctaacctcgg taccttcgct gattcctgat ggatcaccta
      781 gaatcacaat accaacgcta tcttcctcca agttcaaagc cattccggcc actccacctt
      841 cgaattctac caactcattg taggcaacac cggacagccc atgtactcgg gcaataccat
      901 cgcctacttc aatcaccgtc cctacatcca ccataccaag gtcggtaccg aatccttcta
      961 tttgtttttt tattaccgat gctatgtcct gtcctctgac ggccatatca ccctccgttt
     1021 tatgggaata acccgcttat tttgttttcc gaatacatta acctaagatc aagcccccct
     1081 gatgagctct ctgcgcatat tttccaattg ggttttaaca ctcccgtcta ccaatctatc
     1141 ccctactcta gccaggtatc ccccaagtat cgactcatca acgtaggttg taactgacag
     1201 ttctttccca accatccgcg ccaatttacc cactatgtct ctttcctgtg catccgtgag
     1261 tttaaccgca gaaacaatct ctgctcgttc cactcctttt tctgaatcga ctaattcctg
     1321 aaaggcatct gcgatattgg ggagagaagc cacagaattc cgagtagcta acaaagaaac
     1381 aaaattcaat gccatttctg acacagactt tgaaaaagct tctttaatga cttctatttt
     1441 ctttacattt tcaattttcg gggagtctat aaacgcgatg aaatcagaat ttcttgagga
     1501 gtctgcgagc aattctagat ccgcaagcca ttgttcttga tcacctttct cttcggctat
     1561 caaaaatagt gcctgcgcat atcttctcgc tgacaaagcc atatctagtt attcctcgac
     1621 ccttcttcgt tcaaaacgcc ttcaataaga gtgcggtgtc cttcctcatc gagtgaagtt
     1681 tttactacct tttctgcagc tgtaatagcc aagcttgcaa actctttacg aagctcctct
     1741 attgcggcat ctctctctct ctgaatgtta gattctgccc tagatctttc tgcatctagg
     1801 tcctctctta ctttagccat ctcttcttcc ctaaatctat cagcagtctc tcttgcctgt
     1861 gcaactagtt tctgcccctc agatctagct tcctgaagtt gctcctgaat cgcttcttcc
     1921 gatttggctg cctcttcttt tgccttttct gcagcctcta atccggctgt aattttttct
     1981 gctctttgat ccaacattgc taccaaaggc ttatataaga cctttgacaa tatgacaaac
     2041 aatattacaa aactgataat ctgtgttatc agtcctggca gatttattcc aagatctccc
     2101 ataccgaact cctatttatg aagtactcgt cggcacctat accccaatgg caattaagat
     2161 gctaaatgag acccaaccac tagacgaatt taattatgat agcgacaact agagcataaa
     2221 tagcgatagc ttccgtgaac gcaatagcca gaatcatgtt ctgttgtatc tgagcacctg
     2281 cttcaggatt acgacccaag gcttccatgg ccttcgaggc aagcatacca ataccgatgc
     2341 cgggaccgat agctccaagt ccgatcgcga gaccggcacc cacgttcatt atgtctcctt
     2401 ccatttcaaa ctccttaaat tttgattacg tttatatttt cactgaaacg ttctcattta
     2461 atgtttcagt gatgatctcc atcatcgtga cttatgcttg ccatagatgc gaaaactaga
     2521 gtaagcattg caaaaacaaa ggcttgtatt aatccaacga acaattccaa acctaagaat
     2581 ggaattattc ctattaatgg caacaagaat gccattgcca taagaagaac ctcaccggca
     2641 aatatattgc caaataacct aaaagtgaaa ctaatcactc tccctaattc acttatgagt
     2701 tcaagaatac ccacaaaaaa accaatcggc cccgatttaa agcttataaa ttttcccgca
     2761 tgaccgaaga atcccaggat actaaatccc caaaaatgga ccattaccat tgcaataagg
     2821 gcaatcgcta aagacatatt tagatccgta ttggcgctcc tcaaaaaagg aactaagtga
     2881 ccggcccttt tcccctcacc cacattgccg tgattttctc catattgaga aaatgtaagt
     2941 tcctcttcca aagaacctgg tgacaaggtc gctaaaggaa cagatttata atctcgaaaa
     3001 acgtgtagtt taaccgcccc caggtgcgag tcatgatcat ccccatgggt ctcacctgac
     3061 gcatgggatt ttgccttgct ttcttttgac tcaagggtcg tctgcatgag ggctgggtca
     3121 tgatagtcat gggcgttatg gtgatgtgca atttcttccg gtcctgcgat gcggcctaca
     3181 gtaccaaacc caggtaatat ccccatccag tttgctgcaa ctataaaaat aaaaatcgtg
     3241 aaaactagcg ggaaaaatct tctagccttc tctttgccag ctatctgttc gcaaagtgca
     3301 agaaaaaatt ctattattaa ttcaaatagg gcttgtagcc tacttggcac gtctttcaga
     3361 ttttttctta ttaccaaaga gataacgatg agcactaaca tggccaacca agtagtaacc
     3421 atcgtgttca tcaagtccca tttgccaatt ggcgtattaa aaccgtcagt tactttttct
     3481 gcaggaagtt gaattgcggg caaagaagaa gcaaaaaagc cgaaccccaa agtgttacct
     3541 agggctccac ccaatatgct taccgcgagt acggccacgc atattatcaa tatagctaat
     3601 gatctggggt tacccagtat ctttgacact acttttgaaa ctcctactca atcggattct
     3661 gacacatatc taagaactgc catcagcatt cttatcatac cggtcaaagc caccacaaga
     3721 cctatggtga ttccagcaag ggtgagtatt gggctaagtc caaaccggtt atcagcccat
     3781 agacccgccc atgcacctac gcctatgcaa ataccaacgt accacccaac tcccaccaaa
     3841 cgaacaatag ccccatattt tttggtattc ataaggcctc acacgaacgg ttgttaactt
     3901 tatcacaatg tatttctttg ggtcaaaagt tcaataattg ggcctctcaa cccttgttgc
     3961 ctatgtcttc taaatctagc gaatcgataa aatccgtgaa cgccgaaagg ccctctaatt
     4021 cttcttcatt tacctgcttt tcttcagatc ctttcgtgtc ggcactttct ggttccacat
     4081 caagagatac tccagctttc tccaccactt cgtcctctgc atatattggc gcctcagagc
     4141 gaacagccag tgcaagggca tcactgggtc ttgcgtctat ttcaattagg gtaccgttag
     4201 tatcaattac aattttcgcg aaaaaagtat catccttaag ttctgacaca acaatactgt
     4261 gtatagttcc gcccaaatct cctattacag acccaagtaa atcatgtgtc atcggccttt
     4321 gaacttccac ctccatgagt ttgaacctga tagcttcgac ttcaaattgg ccaatgaata
     4381 taggaaggta caaatccgag tctttaactt ttaatacaac ggccctttgc tgggtttctt
     4441 gtctcacacg aatactttca atttcgagtt ccagcaactt gattggcctc ctagcatcgc
     4501 ggtttaatag agaagaatca aatcttaaat acgggaacca agcatgtcaa cgaataattt
     4561 aactttccaa attaatgagg cgaatcctta ttcagttttt ctatgagatc gagttctctt
     4621 ttccaaatct gatcttggta tgataagtct tcgattacac cctcggcaca caattcctat
     4681 atccgcccct atcctgtcta ccctccatat tacctctcca cacggatgtt ttttcttcaa
     4741 ggttatgatg tccccgaggg agtagtccgt taccatatcg tttcttgcca ccttacctgt
     4801 taaataaaac ttcgtcctaa atcttccaag atattattaa tattttctcg aagattcgca
     4861 gctgtatttg cagcagctga cccgaaatta tttcgatcag acgaggcata tataattccc
     4921 cttgaagaac ttacaagtaa tccatggttt tccctaccta gcccaactct aactgatgct
     4981 tctaagtctc cagattgtga cccaattccg ggaaccaata ttggtaattc tcctactata
     5041 tcccttacaa ttttcaattc atcaggatag gtcgctccaa ctaccaaccc taaattccct
     5101 tcattgttcc aagaaacaca atactcagca atgacctgat aaatctttct gtctgtggtt
     5161 ccttttacta caagatcctg taattgatat ccgtcaggat ttgaagattt acaccataca
     5221 aaaacgccac gatccgtgta tctaagaaat ggttcaatgg aatccaatcc agaaaaggca
     5281 ttaacagtaa ctgcatcgaa atcccaaatc tcaaacatag catatgcata tttggaactt
     5341 gtaggtgcta tatcacctct tttagcgtca ccaattatta gaacgtcgga ataattggac
     5401 ttgatgtgat caatcacttt ttcaagggac tttaatcctt ccaaaccaaa tgcctcaaaa
     5461 aagcctaaat taggcttata agccgcagta aacccctcag tgtgatcaat aataagcttg
     5521 caaaactcaa aaacatccga tactggcatc ttaactgggt ctgggtccag tcctatggtg
     5581 aggagggatt tttttgatac tgaaatatct tggagcgaat cagcaaattt tgtcatgttg
     5641 tcctttaaat aagccgtgct atattgttta tgatcttttt tgtaccaaaa atttccgtct
     5701 caacgtaagt attttttctt ttttttggtt tatccctcga ctctcaatat agaccagacc
     5761 tctgtcattt ctgctctggg atgcagtcac tgtcagaact tcactctcag cataaatagt
     5821 atctccatga aatgtaggtc catcatgtgt aacagattcg taaagtaagt tggcgatagc
     5881 cttacctgaa gtttgtcgaa cgctcattcc gacaaccagg ctcatcacca gtaatccgtt
     5941 tacaagagtt ttaccatgtt ggtgttctga catgtaattt tcatctgtgt ggagagggtt
     6001 atcattcatt gtcaataaag aaaataaatg attatccatt tctgtaatgg ttttacctgg
     6061 ccagtgttta aaaatttgcc ctggggaaaa ttcctcaaac caccctccaa aactgtccct
     6121 agaatcgaca ataatttttt ttgaagtcat ttttttcctg cttattaatc aatctgaata
     6181 tttttcaagc aaccgcctag caattactag cttcataatc tcgtttgttc cttcgccaat
     6241 aatcattaga ggtgcatctc tgtaatatcg ttctaccggc aagtcttttg tataaccaac
     6301 cccaccatga acacgcatag cctccatagc aacctctcca caaacctcac tcgcgaacaa
     6361 tttggccatt gcggtctcaa gatcattcct ttccccagaa tctttcttac ttgctgcgtc
     6421 gtagaccatt aagcgagctg catgaacttt tgcagccatt tcagctaact tcaattggat
     6481 tgcttgatgt tctgatatca gtttgccgaa agccgagcgt ttcttggagt atgacaccga
     6541 ggcctctaag gcggcttgtg caacacccac agcccttgca gccacattaa tcctccctgt
     6601 ctccaacccg ttcattacct ggccaaatcc gcgcccttct tctcctccaa taagattgtc
     6661 aacaggcact ctgcagtcgt caaagatcaa ctcgcaggta tccaaacctc tatagcccaa
     6721 tttatccagg tgctgaccta ccttaaaacc ttcagttggt ttttcaaaaa taaaacagct
     6781 tattcctcgg tgtcttggat ttgaattggg atccgttttg gccattaggg cgaaggcatt
     6841 cccattatcc ccgttactaa tgaacatctt ccgcccgttt atcacatatt ctccaccatc
     6901 tttagtagct gtagtggata tgttttgagc atcactgcca gcttcagcct ctgttaaagc
     6961 taacccccct cgaatctcac ctgttgccat tctaggcaaa atacgatgtt tttgctcttc
     7021 agtaccatga atagtgatta tgtagctaag tatatgatgg gttccaatta tccccgacac
     7081 actcatccag cccttactta gctcttcgaa tatcattgcg aacgtcgtaa aatctaggcc
     7141 catcccaccg tattcagtcg ggatattaat accaaacaat cccaattcac acattgtggg
     7201 aattagttca tgtgggtata tgtcttcatt atcatagcta ttcgctattg gttccacgtc
     7261 tctcttcaca aaatctctaa ctaacgaaac gatctgttta cgagcttctt cagaagtttg
     7321 tgtcaccaat ttcctaactc ctaactctag ttaaatcgag tctctatatt gcctgttacc
     7381 tacgttttta gcggaaacgc ccaacacgtt caagaaacct tccgcatcct tgtgatcata
     7441 accccccata gcttccatag agccatcatc tgtgtataag gagaatggta tcttttctat
     7501 tgagtcatcg acagaatcaa agaatatatt gcctttgtac aatttgattt gaatgctacc
     7561 tgtaaccata gaagccaaag gagaaagggc tgccaaggct gaggaggttg cagaatccag
     7621 ccagtaacct tgatatattt gagtactaat taaattagat aaatttgtgt aaaaatccct
     7681 agctctccta tctaaaacga actgcaaaag atattcccaa ctttgcccaa gtaattccat
     7741 accaggggat tcatatatgc ctctagattt aatccccaca aatctgtttt ctatcacatg
     7801 agtgccaatg cccaccccat ttctaccc
//
LOCUS       DASCNS010000236         3109 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001837287, whole genome shotgun sequence.
ACCESSION   DASCNS010000236 DASCNS010000000
VERSION     DASCNS010000236.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001837287"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="QF838_08585"
     CDS             complement(<1..791)
                     /locus_tag="QF838_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HJO60774.1"
                     /translation="MSDQELIFMSAADLATRMKEKQISPVEAVRAYLDRITEVDGKIN
                     AYITVLADQALAEAKQAESDINAGLIKGSLHGVPVGVKDQIYTKDIRTSSASKIRSDF
                     VPDFDATVVEGLKRSGAIILGKLNMTEFAMGDPITSAFGVTHNPWDLTRNPGTSSTGS
                     GAATASFMCATSLGEDTGGSVRGPASNCGLVGLRPSWGRVSRYGVDGASWSLDTIGPI
                     SRTVEDCALTLSAIAGFDQKDQYMRDLPVPDYCAALTGDIKVVKIG"
     gene            complement(797..979)
                     /locus_tag="QF838_08590"
     CDS             complement(797..979)
                     /locus_tag="QF838_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60775.1"
                     /translation="MSELNEDEIRGLAKAVNIEIQNSDITDISYSLNAMLEAIDSINP
                     EGINAVEPLSVIQKED"
     gene            1267..2880
                     /locus_tag="QF838_08595"
     CDS             1267..2880
                     /locus_tag="QF838_08595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJO60776.1"
                     /translation="MQKSDEVDILVIGGGPAGSTAASMAAKSGLSVKVCERSKFPREH
                     VGESLLPASIGILEELGLNEAISNAGFLPKYGATMVWGKDETPWSWRFSETNKQNIHS
                     YQVSRPIFDEILLKRSAELGVLVEEESSVSKILTDDSNRGVVVTNKMGQEYFQPAKFI
                     VDASGQSALLARNFDLRQWDEHFQNLAIYGYFAGAKKLDAPNENNIFIESYENGWVWA
                     IPLNDDTTSVGAVVDSNESNAKLRKTDPITFLKDQLSMTNRTSHMLSEAELKSDVKIL
                     KDWSYTTSKMAGDGWVLAGDAACFIDPLFSSGVHLAMMSGVLASAYAVTCLKDKILGI
                     ESASVYETTFRQEYDHFRDLALLFYSSNRTVDSYFWEARRIFKEENYFSDRISFIRAV
                     SGQSVRGYERVALERGELPNEFMSAMSLHNKKSEDRNAKLLSIQDSILEKIPCLAPEA
                     ELEKKPVLAEGEFEWGTVITTPYRKEGVPCSNFVANLLMEIDGEKSLSKIVEGLSTSA
                     SEDTKKQLLKYSIEAISILFIEGVIDNLD"
     gene            2911..>3109
                     /locus_tag="QF838_08600"
     CDS             2911..>3109
                     /locus_tag="QF838_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60777.1"
                     /translation="MLEDIQGKVAWITGAGTGIGESGAIKLAEAGCKVVLSGRRHDVL
                     KNVASKISGDVSIQPLDVSDND"
BASE COUNT          968 a          636 c          669 g          836 t
ORIGIN      
        1 cctattttaa ctaccttaat atccccagta agtgcagcac aataatctgg aactggtaaa
       61 tctctcatat actgatcttt ttgatcaaat ccagcaatgg cgctcaaggt caatgcacaa
      121 tcctcaaccg tacgagaaat tgggcctatc gtatccaggg accatgacgc cccatcgact
      181 ccatacctac taactcgacc ccaagaaggt cgaagcccga ctaatccaca attggaagca
      241 gggccccgta ccgaacctcc tgtatcctct ccaagtgacg tagcgcacat aaaagacgcc
      301 gtggctgccc cagaaccagt acttgatgtt cctggattcc gagtgagatc ccaaggatta
      361 tgtgttacac caaaggcaga tgttatcggg tcacccatag caaattcagt catgttcagc
      421 ttgccaagga ttattgcccc agatctctta agtccttcga ccactgtcgc atcaaagtcc
      481 ggcacaaagt ctgaccggat tttggatgca cttgatgttc gtatatcttt tgtatatatc
      541 tggtccttca ccccaactgg aactccatgc aaagagccct tgatcaaccc tgcgtttata
      601 tcactttccg cctgtttggc ttccgctaat gcctggtccg ccaaaacggt aatatatgcg
      661 tttattttgc catcaacctc ggttatccta tcaagatatg ctcttacagc ctccacgggg
      721 gaaatttgtt tttccttcat tctagtagcc agatctgctg ctgacataaa aattaattct
      781 tgatcgctca taatttttaa tcctcctttt gtataacaga cagaggctca acagcattga
      841 taccttctgg attaatacta tcgatagctt ccaacatggc attcaagcta tagctaatat
      901 ctgtaatatc agagttttgt atttcgatgt tgacggcctt agctaatcca cgaatctcat
      961 cttcattcaa ttcagacatg taaactctcc tcaaaagtat tactgggttg actcagcgat
     1021 tataggatat gtcggtgaaa gcagttcccc tgttggtctt aagcatgtgc gcatttccaa
     1081 caacttgccg atcagtttgc taatgtcaaa atgtccctcc tattttaaaa gcttgatctg
     1141 aaatccctat tatctttacg gtaatcacct cggcatatga aaaatagacc gtatgaatcc
     1201 aatataatta aagttattct acggtaatga agctagagtg aaacaacata gctgggtatc
     1261 taaaaattgc aaaaatcaga tgaagtagac atattggtaa taggtggagg cccggcaggt
     1321 agtacagcag cctcaatggc agccaaatca ggcctctcag tcaaggtgtg cgaaagatct
     1381 aaatttccaa gggaacatgt aggagaatct cttcttccgg caagcatagg tatattggaa
     1441 gaattaggtt taaatgaagc gatatcaaac gcagggttct tacctaaata tggggcaacc
     1501 atggtatggg gcaaagatga aacaccttgg tcgtggcggt ttagtgaaac caataaacag
     1561 aatattcatt cataccaggt ttcaaggcct atttttgatg aaatactact caaaagatca
     1621 gcagaacttg gtgttttggt agaagaagag tcatcggtat ccaagatact gacagatgat
     1681 tcaaatagag gagttgtggt cactaataaa atgggccagg aatacttcca acccgctaaa
     1741 ttcatagtag atgccagtgg acagtccgcc ctcctagcaa ggaattttga cttgagacaa
     1801 tgggatgaac attttcaaaa cctagcaatt tacgggtatt ttgcgggggc gaaaaagctg
     1861 gacgccccga acgaaaacaa tatatttatt gagtcgtatg aaaatggatg ggtctgggcg
     1921 attcccttaa atgacgacac tacaagtgta ggagcggtag tagattccaa tgagagcaat
     1981 gcgaaattgc ggaaaactga tccgataacc ttcttaaaag accaactatc aatgaccaac
     2041 agaacatcac atatgctttc ggaagcagag ctaaaaagtg atgtgaaaat attgaaagat
     2101 tggtcatata caacctcaaa gatggccggc gatggttggg tattagccgg agatgcggca
     2161 tgttttatag atcctttatt ttcttcaggg gttcacttag ctatgatgtc aggtgtactt
     2221 gcttcagcat atgctgttac atgcttaaaa gataagattc ttggcataga aagcgcatca
     2281 gtgtacgaaa ccacatttcg acaagaatat gaccatttcc gggaccttgc cttattattc
     2341 tattccagca atagaaccgt agattcatat ttctgggaag cccgtcgaat atttaaagag
     2401 gaaaattatt tttctgacag gatctccttc ataagagcgg tgtcaggaca atctgtccgt
     2461 ggatatgagc gggtggcttt agagagaggt gaattaccga atgaattcat gtctgccatg
     2521 agcctacaca acaaaaaatc tgaggataga aatgcgaaat tactttccat tcaagattcg
     2581 atcttggaaa aaataccgtg cctagcacca gaagcagaac tagaaaaaaa gccggttcta
     2641 gcagaaggag aatttgagtg ggggacagtt ataacaacac catatcggaa ggaaggagtg
     2701 ccatgtagta atttcgtggc aaatctattg atggaaatag atggcgaaaa gtcgttaagc
     2761 aaaatcgtgg aaggcctttc aacatctgct agtgaagaca ccaaaaaaca attattaaaa
     2821 tatagtatag aggcgataag tattcttttt atagaaggag ttatagataa tctcgattaa
     2881 ggcaattaaa tagtaattaa ggagaaacct atgttagaag atattcaagg caaggtcgcc
     2941 tggataaccg gagcggggac aggtatcggg gaatctggtg ccataaaatt agcggaagcc
     3001 ggctgcaaag tggtcctttc aggacgaagg catgatgtgc tgaaaaatgt agcctcaaag
     3061 atatccgggg atgtttcgat acagcctcta gacgtttcag acaatgatg
//
LOCUS       DASCNS010000237         2733 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001837329, whole genome shotgun sequence.
ACCESSION   DASCNS010000237 DASCNS010000000
VERSION     DASCNS010000237.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001837329"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="QF838_08605"
     CDS             <1..867
                     /locus_tag="QF838_08605"
                     /inference="COORDINATES: protein motif:HMM:NF025224.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HJO60778.1"
                     /translation="LTNLESLYLDENPISDLGPISSLTNLRWVGFWDTNISDLGPLSG
                     LTTLESLSLGGNSISDLSPISSLTNLESLYLDENSISDLSPTSALTNLRWVGFRNNNI
                     SNLDPLAALSNLNSLDLYQNEISNLAALQSLTSLTYLEIGENPITDYSPITELCNATN
                     TYCSGVPVLTQTTVSYPTDLTVADLAGGGYGGNYQMNGSYNGRPVWTNPLGGDTGYEP
                     RHIFFSPQQVVSSNGTWVIQSGSPTDEWDAGGYYDCQGLPWEECSPGWEGFKLDVTVE
                     ASTAWQEWQDSP"
     gene            1078..>2733
                     /locus_tag="QF838_08610"
     CDS             1078..>2733
                     /locus_tag="QF838_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJO60779.1"
                     /translation="MARCENCGHENPGSNLFCTSCGTSLATENISSHSVSTAPEETLK
                     NPTSTLNSNLNMVLEELRNVLFSIDALRSSDSEILDDSKSNLENRKSELLITLNRVLE
                     SESKFKTPTRIEYFSNFLQEVLESDNSLESESELLDIIRSNDLEVTSDNESKRITVSP
                     PSNPAGIDPSVVEQPIPPFFGWSRLWTTLFSENAMSTFLGLGILLMTVSSLVLLVNWW
                     SDQNLRPYLIVLASVQMLAFIIIGHIVKWKIGLHLSGLAFITIGAVWSIFAAGMLAYL
                     TFDPLTEYPRIPGIDLQVNLQPLAWLMVSGIATPVWLLLAYRYQGHILTQGAAILFTT
                     SSVLIIASFGHRWELWEWAVTTLPLIALMILFLRKYIYRANQSNAMDQLLWTALAIGI
                     ASPLILTVNYLANSVQHPGPIAFSFFFTAISCVLGFRFSGVRWLEHVTALFLPVSAYL
                     FFYNWIDDTAPYIKIILSSIGFIYLYVGLKPRFGELNKIEREWPILRPWFLVSLLTII
                     FVSIPYGVPTWTSVTSMITGAVIAFWMVNKWQGTLWRWIPILPL"
BASE COUNT          753 a          600 c          554 g          826 t
ORIGIN      
        1 ctcacaaact tagaatcttt gtacctggat gaaaacccca tatctgacct tgggcctatt
       61 tcctcgctta caaatttacg ttgggtaggg ttttgggata ccaatataag tgatctaggt
      121 cctttatctg ggctaacgac cctggagtcc ttgagtctag gcggaaactc aatatccgac
      181 ctcagcccta tttcatcgct tacaaactta gaatctttgt acctggatga aaactccatt
      241 tctgacctaa gtcctacatc cgcgcttaca aatttacgtt gggtaggttt cagaaataac
      301 aatataagta atctggatcc tctagctgca ctttctaatt tgaatagttt agatctatac
      361 caaaatgaaa tatcgaattt agctgcgctt caatctttaa cctcattaac ctacttggaa
      421 attggtgaaa atcccatcac tgactacagt ccaataactg aactttgcaa tgcaaccaat
      481 acatattgca gcggagtgcc tgtacttaca caaaccacag tttcatatcc aactgactta
      541 accgtagcgg atctagcagg gggaggatat ggtggcaact accagatgaa cggatcttat
      601 aacgggcgcc cagtttggac caatcctctt ggcggagata caggatatga acctcgccac
      661 attttcttta gtccccaaca ggtcgtgtca tctaacggta cctgggttat tcagtcggga
      721 tcccccaccg atgagtggga cgctggcggc tactatgact gccaaggtct tccctgggaa
      781 gaatgctccc ctggatggga aggttttaag ttagatgtga ctgttgaggc cagtactgca
      841 tggcaggaat ggcaagactc accataatga tgagggtgac ctccggccgt tgccctcagg
      901 aagtaatggc aagaaaactc aactggttta acccaacctt ctgcccacaa cctttgcgat
      961 cgataatctt cttactaatt gttccgaaat tcgcatagtt cctactcatt ttcttgcaat
     1021 aatggttcca agtggtttca agatcacggc tgttgcaaat aggctaagga caatcatatg
     1081 gctagatgtg aaaactgcgg tcacgaaaac cctggttcga atctattctg tacgagttgc
     1141 gggaccagtt tagctacaga aaatatctct agccattctg tttcaactgc cccagaagaa
     1201 actctaaaaa accccacatc gaccctgaac agcaatctta atatggttct ggaagaattg
     1261 cggaacgtcc ttttttctat agatgccctt agaagttctg atagcgaaat actggatgat
     1321 agcaagtcta accttgaaaa tcgcaaatca gagttactaa ttactttaaa tcgtgttctt
     1381 gaatctgaat ctaaattcaa aactccaacc cggatagagt atttctctaa tttcttacag
     1441 gaagttttgg agtcagacaa ttcactcgag tcagagtcag aattattgga tatcataagg
     1501 tccaatgacc ttgaggtaac atcagataac gaatccaaaa ggatcaccgt tagtcctcca
     1561 tcaaacccgg ctggcataga tccgtcggtt gtagaacaac caataccgcc attctttggt
     1621 tggtctcgcc tatggacaac tcttttctca gaaaatgcca tgtccacttt tctaggacta
     1681 gggatattac ttatgacagt gagctctcta gttctattag tgaattggtg gtcagatcaa
     1741 aatttacgac cttatttgat tgtattagcg tcagtgcaaa tgctagcttt catcataatc
     1801 ggacatatag tcaaatggaa gattggtctt cacttatctg gtcttgcctt tattactatt
     1861 ggagctgttt ggtccatatt cgccgctgga atgcttgctt atctgacatt tgatccactt
     1921 acggaatatc cacgaatacc aggaattgat ctacaagtaa atttgcagcc attagcgtgg
     1981 ctgatggtaa gtgggatcgc gacccctgta tggcttttac ttgcctacag gtaccagggt
     2041 catatattga cacaaggtgc cgctatatta tttaccacat cgtcagtttt aattattgcg
     2101 tcatttggac atagatggga attgtgggaa tgggcagtca caactctacc attaatcgcg
     2161 ttgatgattc tgttcctaag aaaatatata tatagagcca atcaaagcaa tgccatggat
     2221 caactgttat ggacggcgct tgcgatagga attgcatctc cattaattct tacggtaaat
     2281 tatctcgcaa attcggttca acatcctggc ccaatagctt tctctttctt tttcacggca
     2341 atcagttgtg tattgggctt tagattcagt ggggttcgtt ggctagaaca tgtaaccgca
     2401 ttgtttctcc ctgtgtctgc gtatttgttt ttttataact ggatagacga taccgcccct
     2461 tatattaaaa taattctcag ctcaatcgga tttatatatt tatacgtagg actcaaacca
     2521 agatttggtg aattaaataa aatagaacga gagtggccga tactccgccc ctggtttttg
     2581 gtttcgctcc ttacaatcat tttcgtttct attccttacg gtgttcctac gtggacaagc
     2641 gtaaccagta tgatcacggg agctgtaatt gctttttgga tggtcaataa atggcaagga
     2701 actctttggc gttggatccc gatcctacct ctc
//
LOCUS       DASCNS010000238         2007 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001841743, whole genome shotgun sequence.
ACCESSION   DASCNS010000238 DASCNS010000000
VERSION     DASCNS010000238.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001841743"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(68..808)
                     /gene="coxB"
                     /locus_tag="QF838_08615"
     CDS             complement(68..808)
                     /gene="coxB"
                     /locus_tag="QF838_08615"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HJO60780.1"
                     /translation="MKNNWTLPLFSWVLSTVILEILAITNLHTLPSSGSKQAVVIDEA
                     FFLLIYICIPIFTLITVFLIYSVFKFRSKGRPDEDGPHVTNSRNLSYVWVIGSFILVA
                     FVFVHPGVTGVLELRAEEKRVPDVVIQAEAQKWSWKIMYLEEGVYSRNEVVLPAYYNT
                     KFYVTSKDVLHAFWIPAFRVKIDAVPGMTTHVNATPTKLGNFDEDVNYRLQCAELCGI
                     GHAQMSIPVRVLSENDYKTWLTNQKPLR"
     gene            complement(1099..1797)
                     /locus_tag="QF838_08620"
     CDS             complement(1099..1797)
                     /locus_tag="QF838_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HJO60781.1"
                     /translation="MKKVGVLIADDDPRICAFISSHLSKRESIKTAVVHDGQSALEQA
                     ALQQPELVILDLSMPIMDGMTALLKLRDWYSGAIMILSATDEEAQKVQALDIGADDYL
                     TKPFGIEELLARVRTLLRRQREIDSVGQESKPVLTFGNITADLSSRIVLKDGVEINLT
                     RTEFELLKTLATNKGKIISHRELLQKVWGPEYGGEHEYLRTFIKQLRKKIEQEPSRPK
                     FILTQPGMGYRLVG"
     gene            complement(1824..>2007)
                     /locus_tag="QF838_08625"
     CDS             complement(1824..>2007)
                     /locus_tag="QF838_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJO60782.1"
                     /translation="QANIFDKFYRLATSQQNGKPGAGLGLAICQYICKLHGSILTVQS
                     KVGEGTVFKFSFPSIG"
BASE COUNT          576 a          432 c          396 g          603 t
ORIGIN      
        1 ccataagaac ttaagttgcg tcatttgtag atctccgtag tttagggaca aatatcagtt
       61 tattggttca tcgaaggggt ttttgattcg tcaaccaagt tttgtaatcg ttctcggata
      121 aaactctaac cgggatggac atttgagcat gtcctattcc acataattca gcgcattgaa
      181 gtcgatagtt cacgtcctca tcaaagttgc ctaatttagt cggggtagcg ttgacatgtg
      241 tggtcatgcc tggaactgcg tcaattttca ctctaaaggc cggaatccaa aaagcatgta
      301 aaacgtcttt ggaagtgacg taaaacttcg tattgtaata ggcaggtaag acgacttcat
      361 tccgactata cacaccttct tcgagataca tgattttcca actccatttt tgcgcttcag
      421 cttggataac tacatcaggt acacgctttt cttccgctct caattccaga acaccggtaa
      481 cacccgggtg gacaaacacg aaagcaacca agataaatga cccaatcacc caaacatagg
      541 aaaggttacg gctatttgtt acgtgtgggc catcttcatc tggcctacct tttgaccgaa
      601 atttgaagac gctataaatc aaaaacactg taatcaaagt gaaaatagga atacatatgt
      661 atatgaggag aaagaaggct tcgtctataa ccacggcctg ttttgaacca ctagaaggaa
      721 gggtatgtaa atttgtaatg gcgagaattt caagaattac ggtgcttaga acccagctaa
      781 agaggggcag cgtccagttg tttttcattg gcttccttct acaaggaaaa acatcggctg
      841 ctgtgaactg ggagatatgc tcatataaat tttcgtgccg atccttgcaa gcttgtatgt
      901 ttaattcccg ccaaaaccca tccccttgcc tttcttgcaa tcagcaagag gatgagtggt
      961 ttgacgtccc gtaccaggaa ggtgcttctt taatacgaga cgacaacagt ctagttgaac
     1021 cggtgtgaag ggtgtgtgaa acctagaagg ctaatatgaa atttatatga atattcagca
     1081 attttctgtt ttctgggtct atcctacaag gcggtaaccc attcctggct gggtaagaat
     1141 aaacttgggc ctagatggtt cttgttcaat tttctttctt aactgtttga tgaaagtccg
     1201 aaggtattca tgttcaccac catattctgg accccatact ttttgaagca attctctgtg
     1261 ggaaattatc ttacctttat tggtcgccag tgttttcaac aattcaaatt ctgtccgagt
     1321 gagattgatt tcaactccat ctttaagcac tattcttgag gacaaatcag ccgtgatatt
     1381 accaaaagtg agcacgggtt ttgactcctg accaaccgaa tcaatttctc tctgccgtct
     1441 gagtaaagtt cttaccctgg ccaaaagttc ttcgatccca aatggtttcg tcaaataatc
     1501 atctgcccca atatcaagag cctgcacctt ttgagcctcc tcatccgttg cggacaagat
     1561 catgattgcc ccagaatacc aatctcttaa tttcagcaag gcagtcattc catccataat
     1621 aggcatagat agatctagaa taactagctc tggttgctgt agtgccgcct gctctaaagc
     1681 cgattggcca tcgtgaacga ctgctgtttt tattgattct cttttagata aatgacttga
     1741 tataaaagcg caaattctag ggtcatcatc tgcaatcaat actccgacct ttttcatatc
     1801 ttctccatta ataaatgtaa gtatcatcca atggaaggga aagaaaattt aaatacggtt
     1861 ccttctccaa ctttgctttg aactgtcagg atcgaaccat gtaacttgca tatatattgg
     1921 cagattgcta aacctaaacc agccccaggc ttaccatttt gttgagaagt ggccaggcga
     1981 taaaatttgt caaaaatatt agcttgt
//
LOCUS       DASCNS010000239         4680 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001841859, whole genome shotgun sequence.
ACCESSION   DASCNS010000239 DASCNS010000000
VERSION     DASCNS010000239.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001841859"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="QF838_08630"
     CDS             complement(<1..112)
                     /locus_tag="QF838_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HJO60783.1"
                     /translation="MPDSETVTKDDVLEALRDVYDPEIPVNIVDLGLVYGV"
     gene            complement(220..2205)
                     /gene="uvrB"
                     /locus_tag="QF838_08635"
     CDS             complement(220..2205)
                     /gene="uvrB"
                     /locus_tag="QF838_08635"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HJO60784.1"
                     /translation="MPEFKLSADFTATGDQPTAVSQLSKGISKGFGKQTLLGVTGSGK
                     TFTMACIVEKIQKPTLVIAHNKTLAAQLATEFKEFFPENAVEYFVSYYDYYQPEAYVP
                     RTDTYIEKDADINEELDKLRHSATRSLLTRKDVLIVASVSCIFGLGAPEEYQGFVASV
                     KKGESKNQRDLIRKLVDMQYERNDYDLARGRFRVRGDTVEILPAYEQLGIRIEFWGDE
                     VEKITHLDTLTGEILSEKEYVEIYPAKHFVTSKEKLRMAIRDIGEELELHLKHLKSSD
                     KLLEAQRLESRTKYDLEMLEIAGYCAGVENYSRHLAQRAAGSTPYTLLDYFPDDFLLF
                     VDESHMTLPQLRAMYRGDQSRKETLVEYGFRLPSALDNRPLNFGEFEKRVSQVVFVSA
                     TPGPYEMENSSATAEQVIRPTGLLDPFIEVKPVTGQIDDLLDQIDTRVKKGERILVTT
                     LTKRMSEELSDYLKESGIRTQYLHSEIDTLERSEILRDLRMGVYDVLVGINLLREGLD
                     LPEVSLVAILDADKEGYLRSRTSLIQTIGRAARHIDGKVIMYADKMTDSMKNAIEETE
                     RRRLIQQSYNEENGITPQGIRKAIRDITERVKSVVEPSRENLINGLELPKEDLVRLIK
                     DLELQMKSASKDLEFEKAAIVRDQILDLKKIVAEEPR"
     gene            complement(2217..2975)
                     /locus_tag="QF838_08640"
     CDS             complement(2217..2975)
                     /locus_tag="QF838_08640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO60785.1"
                     /translation="MNTDYDSWADIYDEVYSFVDYDINFYLQKASGIKGNILEIGCGT
                     GRITIPLMENGATITGIDSSQRMIERLETKTASTKVPIKIIRQDVRTLELSQKFSLVI
                     FPYRGFQSLLTVEDQIKALNSLRNHLKPGAHLIITLFVPSRDLFDQRPDIFYHLRNSR
                     HKKGNGYRSLHHRTEFDRHNQLLHTQISITEHIDNHLISKRYADFTLKYLYTNEARYL
                     FEYCGFRIEEIAGDYDGTPYGRGSEETIWSLVVK"
     gene            complement(2972..3556)
                     /gene="ruvA"
                     /locus_tag="QF838_08645"
     CDS             complement(2972..3556)
                     /gene="ruvA"
                     /locus_tag="QF838_08645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HJO60786.1"
                     /translation="MVNTLISAVTGRLISKGSEYVDVEMAGITLRISAPVTTVENIGN
                     LGSEVKILTSLQMRQDSITLYGFITENDRNAFDTLITISGVGPRLALAILSTFDAASL
                     AAAVSSEDVNAFKSVSGVGNRTANRILLELKGKMEQTWSIPNDSGELDDVFDSLTALG
                     YSIQEARAAIGSINSENLSTEEKIRMALENITNR"
     gene            complement(3550..4050)
                     /gene="ruvC"
                     /locus_tag="QF838_08650"
     CDS             complement(3550..4050)
                     /gene="ruvC"
                     /locus_tag="QF838_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HJO60787.1"
                     /translation="MRVLGVDPGTIKMGLGVVDSISGDLNSPYHGLITPRRKDPLENR
                     LAQLFEAVKKAIEENQPDAIAVEEPFVSKNPKTAIAIGQAQAVVFIAGAQKDIPVFKY
                     APQEVKKSVTNYGGSSKEQVSEMVSLLLGGRDIQGPDDVTDALAVAICHINSHMLDDL
                     IGSSTW"
     gene            complement(4064..>4680)
                     /locus_tag="QF838_08655"
     CDS             complement(4064..>4680)
                     /locus_tag="QF838_08655"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HJO60788.1"
                     /translation="RLRMAIQIAKDNNMPADNIDRAIKRGTGESSEGVAMAEIVYEGY
                     GPGGIGIMVETLTDNKNRTVSDIRSTLSKAGGNMAENGAVAWQFEQKGVIVVDVEAND
                     AENLALAAIDAGADDFETYDGTLHVYSEPTSLENIRSTLTNSEAKVSSTELSMIPNNT
                     IMLDSKKAIQTLRLLDSLEELDDVQKVYSNADFPEDALDNFKAS"
BASE COUNT         1205 a         1048 c          871 g         1556 t
ORIGIN      
        1 caactccata caccaatccc aagtccacaa tattcactgg tatctccgga tcatagacat
       61 cccgaagggc ttccaataca tcatcttttg tgactgtttc tgaatcaggc atacttcttc
      121 cttaatttag cgtactcact tcttgaccta tttagtccag aataaagttt atattttgag
      181 ttcttacagt gtcaacaaac aaccgtaaga aatttttacc tatctgggtt cttcagcaac
      241 tattttcttt aaatccaata tttggtccct gacaatagca gccttctcaa actccaaatc
      301 ttttgaagca gatttcattt gtaattcaag gtcctttatc aggcggacta aatcttcttt
      361 gggcaactcc aggccattaa taagattttc ccgtgagggt tctactactg acttgaccct
      421 ttcagtaata tctctgatgg cctttcggat gccttgaggg gtaatcccat tctcttcgtt
      481 gtaggattgt tgaatgagac gccttcgttc agtttcttct atggcatttt tcattgaatc
      541 tgtcatctta tcggcataca taatcacttt tccatcgata tgtctagcgg cccgtccgat
      601 agtttgtatt agggaggtcc tagaccttaa ataaccctct ttgtctgcat ctaatatggc
      661 taccaaactt acttcaggaa gatctaggcc ttctcgtaat aggtttatgc ccacgagtac
      721 atcgtataca cccattctca aatctcttaa tatttcactt ctttcaagag tatcgatttc
      781 agagtgcaaa tattgggttc ttattccaga ttccttcaaa taatccgata attcctcaga
      841 cattcttttt gttaaggttg ttaccagaat tctttcccct ttcttcactc tagtgtcaat
      901 ttggtctaac agatcatcaa tctggccagt tactggttta acttcaataa aaggatctag
      961 aagcccagtt ggccttatta cctgttcggc tgtagccgag ctgttttcca tttcatacgg
     1021 acctggggta gctgacacaa aaactacctg acttaccctt ttttcgaatt ccccaaaatt
     1081 taaaggtcta ttgtctagtg cagatgggag cctaaatcca tattccacta gggtttcttt
     1141 cctagattgg tcaccacggt acatggccct gagttgagga agagtcatgt gagattcatc
     1201 cacaaaaaga agaaaatcat ctggaaaata atctaataag gtatacggtg tgctccccgc
     1261 tgccctttgg gcaagatgcc ttgaataatt ttccacccca gcacagtagc ctgcgatttc
     1321 cagcatttcg agatcatatt ttgttctgga ttccagcctc tgagcttcca gcagcttatc
     1381 tgaagattta agatgtttca gatgtaactc gagttcctcc ccaatatccc taatcgccat
     1441 tcgtagtttt tctttggaag taacaaaatg ttttgctgga tatatttcca catactcttt
     1501 ctcacttaat atttctccag ttaatgtatc taggtgagtt attttctcaa cttcgtctcc
     1561 ccaaaattca attctgatac caagttgctc ataggcaggt aatatttcta cagtgtcgcc
     1621 tcttactcga aatcgccctc ttgctaaatc atagtcattt ctttcatatt gcatatcgac
     1681 taattttcta attaaatctc tttggttttt ggattctcct ttttttacag atgctacaaa
     1741 tccttggtat tcctcaggag cacccagccc aaatatgcag gaaacagagg caacgatcag
     1801 cacatccttc cgggttaaca gtgaacgggt cgctgaatgc ctaagtttat ccagctcttc
     1861 attgatatca gcatcttttt caatgtatgt atcagttctg ggtacgtagg cttctggttg
     1921 gtagtagtcg tagtaactta caaagtattc gacggcattt tcaggaaaaa attctttgaa
     1981 ttcagtagcc aattgggcag ccaaggtttt gttatgagcg atcacaaggg ttggtttttg
     2041 aattttttcc actatacagg ccatagtgaa ggttttaccg cttcctgtaa cccctagcaa
     2101 tgtttgttta ccaaaaccct tagatatgcc tttggaaagt tgagagacag cggtgggctg
     2161 atctccggtt gcagtaaagt cagcggataa tttaaattct ggcatatacc ttccagttac
     2221 ttaactacta gtgaccaaat tgtttcctca gaacctcggc cgtatggagt tccatcgtaa
     2281 tctccggcaa tttcttctat tctgaaacca caatattcga aaaggtaacg tgcttcattg
     2341 gtataaaggt atttcaaggt gaaatctgca taccgtttag atatcaagtg gttatcaata
     2401 tgttcagtta tagaaatttg agtatgtagc aattggttat ggcggtcaaa ttctgttcgg
     2461 tgatgcaagg atcggtaacc attgcctttt ttgtgccttg aattcctcag gtggtaaaag
     2521 atgtcaggtc gttggtcaaa taaatcccta ctaggtacaa aaagcgtgat aattaaatgg
     2581 gcacctggtt taagatgatt tctaagagag tttaaagctt taatctgatc ttctactgtc
     2641 aagagagatt ggaaacctct atacgggaaa atcactaggc tgaatttctg ggacagttcc
     2701 aaggtgcgaa catcttgcct gataattttg attgggactt tggtacttgc cgttttggtt
     2761 tcaagccttt caatcattct ttgtgaagaa tctatacccg ttatggtcgc tccattttcc
     2821 attagaggaa tggtgattct tcctgtaccg cacccaatct ctaatatatt gcctttgata
     2881 ccggacgctt tttggaggta gaaattgatg tcataatcca cgaatgagta cacctcatca
     2941 tatatatcag cccatgaatc ataatctgtg ttcatctatt ggtgatgttt tccagagcca
     3001 tacggatttt ttcttcggta cttaaattct cagaattaat ggaaccaatg gctgctcttg
     3061 cttcctgtat cgagtaacct aacgctgtta aggaatcaaa tacatcatct agttcacccg
     3121 aatcatttgg gatagaccag gtttgttcca ttttgccttt tagctcaagt aaaatacgat
     3181 tggctgttct gttaccaaca ccagagacag atttaaatgc attaacatct tctgaagaga
     3241 ctgctgcagc tagagatgca gcgtcaaacg ttgatagaat tgccaatgcc agccgaggtc
     3301 ctacgccact aattgttatt agagtatcaa aggcatttct gtcattttct gttatgaaac
     3361 cgtacagggt tatagaatcc tgtctcatct gtaaagaagt tagaattttt acttcactcc
     3421 ctaggttccc tatgttttct accgtagtta caggggcact tattcttaaa gtaattccgg
     3481 ccatctccac gtctacgtac tcacttcctt tggaaattag tcggcctgtt actgccgata
     3541 ttaatgtgtt caccatgtgg aactccctat taaatcatct agcatgtgag agttaatatg
     3601 acatattgct acggctaagg catctgtgac atcatcaggg ccctgtatgt ccctaccacc
     3661 cagcagtaag ctcaccattt cactcacctg ttctttggaa ctacccccat aatttgtcac
     3721 agattttttc acttcttgtg gggcatattt aaacactggg atatccttct gtgctccggc
     3781 aataaatacg acggcttgcg cctgtccgat agcaatcgct gttttagggt ttttagagac
     3841 aaaaggctct tctaccgcga ttgcatcggg ttggttttcc tctatcgctt ttttaacagc
     3901 ctcaaacagc tgtgccaatc ggttctctag agggtccttt cgcctcgggg taattaaacc
     3961 atgataaggc gaattcaaat cccctgaaat tgaatcaacg acacctagac ccattttaat
     4021 ggtcccaggg tcgacaccca gaactctcat ttaaatccta atgttaactt gctttaaaat
     4081 tgtccaacgc atcttcagga aaatcagcat ttgagtaaac tttttgcaca tcatccaact
     4141 cctctagcga gtcgaggagt ctgagtgttt gaatggcttt tttactatct aacattattg
     4201 tattgttggg tatcatcgac agttccgttg atgatacttt tgcttcactg ttagtcaagg
     4261 tagagcgaat gttttccaga ctggttggct cagagtagac atgtaatgtt ccatcgtagg
     4321 tttcgaaatc gtcggctcca gcatcaatgg cggctaacgc taaattttct gcgtcatttg
     4381 cctcgacatc cacgacaatg actcctttct gttcaaattg ccaagcgacg gctccattct
     4441 cagccatatt tccgcctgct ttcgaaaggg ttgatctaat gtctgacaca gttctatttt
     4501 tgttatcggt tagtgtttca accattatcc ctatcccgcc ggggccgtag ccttcgtaga
     4561 ctatttcagc cattgcaacc ccctcacttg actctccagt cccacgtttt atcgccctat
     4621 caatgttgtc tgctggcata ttgttatctt tggctatttg gattgccatc cttagacgga
//
LOCUS       DASCNS010000240         1175 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001841876, whole genome shotgun sequence.
ACCESSION   DASCNS010000240 DASCNS010000000
VERSION     DASCNS010000240.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001841876"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="QF838_08660"
     CDS             complement(<1..884)
                     /locus_tag="QF838_08660"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJO60789.1"
                     /translation="MTYFNSRRSAVIAKNGAVATSQPLAAQVGLQILQKGGNAVDAAV
                     ATAAALNVLEPMSTGIGGDMFALIWMADTKKVTAINGSGHSASGANPEDVISKGYSSI
                     PNNGPDAGLAVSVPGTVDGWQKCLDRHGTMTLKEVLQPAIDYAENGYAVTDVIAYQWG
                     QAEAKLKQRPSGAEMLSNNKTPRQGDFIKLDTLGKSLKIIAEDGPEGFYKGDIARKIS
                     NYVQSEGGWLTKEDLANHSSDWDDPICTNYRDVTVWECPPNGSGIAALMAMNIAEGFD
                     IASMGSQSADRYHYLIESL"
BASE COUNT          283 a          273 c          244 g          375 t
ORIGIN      
        1 agagactcaa ttaaatagtg atatcggtct gccgattggg aacccataga agctatgtca
       61 aatccctcag cgatattcat cgccattagc gctgcgatac ctgaaccgtt tggcgggcat
      121 tcccacacgg ttacatctct atagtttgta catatagggt cgtcccagtc ggaactatgg
      181 tttgctagat cttctttagt taaccagcca ccttccgact gcacatagtt agatattttt
      241 cgtgcaatat ctcctttgta aaacccttct ggcccatctt ctgcgattat ctttaatgat
      301 tttcctaaag tatctagttt tatgaaatca ccctgacgag gggttttgtt atttgagagc
      361 atttccgcac ctgagggcct ttgctttagc ttggcttcag cttgccccca ttgataggca
      421 atgacgtctg taactgcata gccgttttct gcatagtcaa tcgctggttg cagtacctct
      481 ttcaaggtca tagtgccatg tctgtccagg catttttgcc aaccatccac agtccccggc
      541 acgctgacag ctaatccagc atcaggacca ttatttggga tggagctgta gcctttagag
      601 atcacgtctt caggatttgc gccggatgca gagtgaccac taccattaat ggccgttact
      661 ttttttgtat cggccatcca aatcagcgca aacatatcac caccgattcc agtagacata
      721 ggttccagta catttaaagc tgcagcggtg gcaacagctg catcgacagc atttccacct
      781 ttttgaagga tttgtaaacc tacctgagct gcaagtggtt gactggtagc aaccgccccg
      841 ttttttgcta ttactgcgga cctgcgagaa ttaaagtaag tcaatttact ggcccccttc
      901 caaaaatgtt gttagtgttg ttagtaatga aatggattat acacattcca attctgtcgc
      961 ggggctgttg acctagattt gtccgattca gtggtgaatt agcctacttt ttctataggt
     1021 gtgattgata ttaaggcatc accaacgggt tttaccaaat tttgtactgc agcaaaatta
     1081 ttggatcgac tcagccactg attttcgctt ccttgctcca atataactgg cattctattg
     1141 tgtatcttag acatgatttt gttagatttt gtggt
//
LOCUS       DASCNS010000241         1182 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001849039, whole genome shotgun sequence.
ACCESSION   DASCNS010000241 DASCNS010000000
VERSION     DASCNS010000241.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001849039"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="QF838_08665"
     CDS             <1..718
                     /locus_tag="QF838_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012373.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HJO60790.1"
                     /translation="MRGAAMLISYEIPMALGITSVLILSGSLAMTEIVRSQSVWFILV
                     MPMGFFVFMAASTAEMSRTPFDQIEAESELGSGYNTEYSGIKFAVLFLAEFMAPIVTA
                     AVVTTLFLGGSQGFDFFPGGIWFALKMFIVIFVLLWFRSTWPRLRIDQIMGFAWKTLF
                     GLGLFNIFLVAAEFTLAREFNQIHDGSLTTDYMLIMAAVNWFITIAVILIMANVVGRK
                     KYTRPEPTPSPLANMGMGEN"
     gene            759..>1182
                     /locus_tag="QF838_08670"
     CDS             759..>1182
                     /locus_tag="QF838_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJO60791.1"
                     /translation="MKNLVSPSRQFNIVQYPNRRASPFDLAKIDNQNPVSFIATHPLK
                     TFRSLLGLETITDRMPQHANFRGEEFAWYDRRCTGCASCAKYCPLGIIKIVTGTSGVN
                     QQEGQSYDIDVFDIDIGRCMFCGLCVEACPYDALHMGSG"
BASE COUNT          332 a          219 c          276 g          355 t
ORIGIN      
        1 aatgcggggc gcagcgatgc taataagtta cgagataccg atggcactcg gtattacaag
       61 tgtacttata ctttccggat ctttagcaat gaccgagata gtccgcagtc aatccgtctg
      121 gtttatttta gtaatgccca tgggcttttt tgtattcatg gcagcgtcca cagcagaaat
      181 gagtcgaaca ccctttgatc aaattgaagc ggagtcagaa ttgggctccg gatataacac
      241 cgaatacagc ggaataaaat tcgctgtttt gtttctggct gaatttatgg ctccaatagt
      301 caccgccgca gttgtgacga ctctgttttt aggtggctct caaggattcg atttttttcc
      361 tggcggtata tggttcgcct tgaagatgtt tattgtgata ttcgttcttt tatggtttag
      421 gtctacctgg cctagattgc gaatagatca aataatgggg tttgcttgga agaccctgtt
      481 tggacttggc ttatttaata tatttttggt tgccgcggaa ttcacactgg ccagggaatt
      541 taaccagatc catgatggct cactaaccac agattacatg ctgattatgg ccgcagttaa
      601 ttggtttata acaatagcag ttattttgat aatggcgaat gttgttggaa ggaaaaaata
      661 tactagacca gaaccgacac cgtcgccact tgcaaatatg ggcatggggg aaaactaaaa
      721 tgtatggatt aggtttgttc aaggggcttg gagtgactat gaaaaactta gtgtcaccaa
      781 gccgacaatt taatatagtt cagtacccta atagaagagc tagtccgttt gaccttgcca
      841 agatagataa tcaaaatccc gtatctttca tagcaacaca tccattaaag acttttagat
      901 ccctactggg acttgaaaca ataaccgatc gaatgcccca gcacgccaat ttcaggggag
      961 aagaatttgc ctggtatgat cgtagatgca cgggatgtgc aagttgtgcc aaatattgtc
     1021 ctttaggaat aataaaaatt gtcactggca ctagtggtgt aaatcaacaa gaaggtcaaa
     1081 gttacgatat agatgtattt gatatagaca taggtcgatg catgttttgc ggtttatgtg
     1141 ttgaagcttg cccttatgat gcgctccaca tgggcagtgg at
//
LOCUS       DASCNS010000242        12027 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001852699, whole genome shotgun sequence.
ACCESSION   DASCNS010000242 DASCNS010000000
VERSION     DASCNS010000242.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12027)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001852699"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="QF838_08675"
     CDS             <1..649
                     /locus_tag="QF838_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013430.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HJO60792.1"
                     /translation="GKLLSGVPGSGNCSAFIIGGGTVGFNAATALMGAGADVTVADIN
                     LDRIREIDTLTNGHIKTIYPTPAALAEKIQRSEVVVGAVLVPGAKAPKLVSRDMVKDM
                     MPGGVIIDVAIDQGGCVEDIHPTSHQTPTYIQDDVIHYAVTNMPGIVGHTASISLSNA
                     TLPYVRSIAKNGVYKAVTLNSSLAKGVNTIGGQITYPALAESLSMTFSNLEEMLP"
     gene            680..1393
                     /locus_tag="QF838_08680"
     CDS             680..1393
                     /locus_tag="QF838_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJO60793.1"
                     /translation="MQPDRHIVTAKVANIKQETTSTKTFDLIPYKGKIDFLPGQWIDL
                     FVSLNGREQVAGYSITSTPCNTEFISLAVKNTGDDQVTQYIHQSMLEGDTVSFSTGGT
                     CVYNPEQGNAILIAGGIGITPLVSIFRYIGEATNHRATIVHSVSSSEELLFTRELENI
                     CLLKPDQLSYAYTISSGSHHSTKGFSGRISAEMLDQLDVSSFNIAFVCGPDSFVDSLS
                     AQLQKTGFNKGQIKSETWW"
     gene            complement(1444..2571)
                     /locus_tag="QF838_08685"
     CDS             complement(1444..2571)
                     /locus_tag="QF838_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO60794.1"
                     /translation="MKIKDIKISEFEIKDNTPYFKLRKIDSSWEKYDLRSSVGYVHVM
                     HVITDDGLEGICTVGDARYTTITVNALAHLKHLTVGEDPTKKEYLFDMLTKATRMVFM
                     PPGWFGGFDNCLWDIEGQIKSKSVAALISDSNDQVTAYYNYRGADLNALIDDANVGIE
                     KGFTVLKDHFAWNAAQNINSFESVKGVVGEDITLLHDAAACSYTLHEALEVAKQLSQL
                     NFGWFEEPLNDRELGSLKTLCSQSEVPILALETLMNDFVLMREWVREGAVNIVRGNAR
                     HGTTGIVRIAEDLARKGMKIELNGPGGLFGHVHAQLAVGINNTSYYEYFPDGSRDSIG
                     KEIGLLNPPVPEKGKVKLTERPGWGYEFDWKYFNKKRVRVF"
     gene            complement(2646..2771)
                     /locus_tag="QF838_08690"
     CDS             complement(2646..2771)
                     /locus_tag="QF838_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60795.1"
                     /translation="MDSVDAFEFIGRMAQTRQKQDEETKDLSKEEDKEKSDIDSP"
     gene            complement(2824..2898)
                     /locus_tag="QF838_08695"
     tRNA            complement(2824..2898)
                     /locus_tag="QF838_08695"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2864..2866),aa:Thr,seq:tgt)
     gene            3074..3592
                     /locus_tag="QF838_08700"
     CDS             3074..3592
                     /locus_tag="QF838_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60796.1"
                     /translation="MSVEIVIYGAQIITGLATLAVAFVLVFQLKQQRSVAQRELVLAI
                     NQQRQDLAVAVANNTELSDINSRGSHDFADLNNQSERIRFNRLFAAEMSLSNIAQEYA
                     DLLHVDPDLALRTSFALFPGRRKFYKESLIRFTLPSEFVEKVDEHIKEIEDSVGEDGQ
                     DVSVLDPEMRNS"
     gene            3608..4168
                     /locus_tag="QF838_08705"
     CDS             3608..4168
                     /locus_tag="QF838_08705"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJO60797.1"
                     /translation="MELLRYLPENNIEIGGKVPDFLLVDHENKTLRLSDLSGSPAVLY
                     FYPKDGTSGCTQEACDFRDVYTDFLELDCPVYGISPDTEASHQGMAKEHDLPFDLCVD
                     PDLNTIKGYGVWGEFQLNDSEAEYAKGFGFHISENLRAWSVVRCTFLIDKNGILVHKW
                     MNVNFRGHASSVRDQLHKSFKFDIAF"
     gene            complement(4200..5471)
                     /gene="obgE"
                     /locus_tag="QF838_08710"
     CDS             complement(4200..5471)
                     /gene="obgE"
                     /locus_tag="QF838_08710"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HJO60798.1"
                     /translation="MIDNVLIQIRSGSGGDGAISGRHEKFVPRGGPDGGDGGRGGNVI
                     IKGDHNENTLINFRYIKNFIAGNGENGSKAKKHGKDGKDTIIKVPVGTQIIDEGGSII
                     ADIILSGEEYIAAAGGRGGYGNVKYSSSTSQYPVIAQSGELGSEHNLRLELKLIADVG
                     IIGVPNAGKSSLISFLTAARPKVASYPFTTIEPVLGLVKHREKDFVMVDIPGLLEGAH
                     QGIGLGHEFLRHIERTRVLVHLVDGTSDNPSGDFHKINKELELFDESLKDKPQILVIN
                     KVDLEEVRVLAEDVRDSMGEDAWRFHIVSAISGEGVSEMMDEVVQVLSDQPIANHPEP
                     SEDPEEKNIPILRPQPRRRGVSVHQEDGIYIVEAPGVERIAQRIDYEDWLARMQFYKH
                     MQKTGIVKALEEAGITEGDTVRIGDVEWEWD"
     gene            5778..7133
                     /locus_tag="QF838_08715"
     CDS             5778..7133
                     /locus_tag="QF838_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012432.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HJO60799.1"
                     /translation="MEEMAPYIKNKTTVSEMFSEANLFQSYLDVEAALARAQASQGII
                     PAKAAEIISEKAKVEFLNLQNIYDGLDKTGHPLVPLIWELDRICGEEAGGYIHWGATT
                     QNITQTGKLLLIKKCHNSYLEDLGSLLHILGQLAETTKDYAMPGRTHGQHAVPATFGY
                     KVAVWIDELIRHVDRLQASERRVFVAMLGGGAGTMASVGLEGLETQRLIGIELGLGSM
                     TMPSRVIGDHLTEYITILAMLAATSTKMAREIYTMMKQEFGEVEEPVPVGAVGSSTMP
                     QKRNPRLCQDVVAWASEIRTFVPMSLEAMQTEHEADKTTSMMINSAIDRTCVLMGQIT
                     KGLVEIFTDLKVFPDRMRQNLDLSGGLILSERIMLELGRKMGRQKAHDAVYEAAQRAV
                     NEGRPFSQTLQEEPEVASQLTKEEILRLLDPEEYTGLCSYFAEEFALKAEQKSKELID
                     N"
     gene            complement(7138..8334)
                     /locus_tag="QF838_08720"
     CDS             complement(7138..8334)
                     /locus_tag="QF838_08720"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60800.1"
                     /translation="MTKLPLDGIRVLDLCIVLAGPTCGKTLAQYGAEVIKIDPEHRPP
                     QLTPWLDVGRGKRSISLNIVNPDGLETFLKLVDSADVILEGFRKGVTDRLGIGYQQLK
                     ARNPNLVYASINCFGRSGPWEFRPGFEQNAQAATGVQLRNSGGKGQPRPATFTLNDYG
                     TGIAAAYGIMMALLEREKTGRGQQVEAALSYTSATISGQYHVTHQNYVRNDIGGPGVR
                     GLNALTGLYESSDSWVFLSISSNREWETLCRIEEFSNLGLQEEFLSDDLRSKNDLLLR
                     EAFEKIFVANSTTYWIETMSSIRIPLVKNTTAAEIHGDEFNHERGLIKDKDYTNSMDL
                     NSSWGNTSWAGNPVIMSETPLQDVNPPIFGGDTVPILKELGLTTEEIARLRETGAIPE
                     VLPIKI"
     gene            complement(8331..9455)
                     /locus_tag="QF838_08725"
     CDS             complement(8331..9455)
                     /locus_tag="QF838_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60801.1"
                     /translation="MNRIGPLTGYRVIEFSSYPVGSILGMLLSDQGAEVVRIDSPKDY
                     PMRFEGHAISIWNRGKQSLEINVSDHQGQEEFIKHTAGADVIIYDNHPDCYKGFNLSY
                     NSLAKDNPGLVCVSLPGFPKGHIYEDLPPDEGIVASASGIYSFNPSGEFPVAGEGPSF
                     HGLPYASTFAAITASTAVVAALYHRKKQGTGQQITVPVHDAMYQGMGTALVRHSKRST
                     GNQEGHPVIKRFYQCKDKRWINVNISFSRFLKPFLDNIGHTEWLEPLTDINNLLSNET
                     DRNRWINQFTKIWKNKPALEWENIMDELGIPGTMCRTIDEWLDSEHSMASGATVTIED
                     PTFGKMKQAGKIVRLYNHDHGTLASARASQTEKPQPEVNR"
     gene            9588..10223
                     /locus_tag="QF838_08730"
     CDS             9588..10223
                     /locus_tag="QF838_08730"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HJO60802.1"
                     /translation="MKNTINLPIEIVIATRNSGKMDEMSEMLSNSAYKPLSLEQVRVV
                     EEVEETGTTFKENAILKAETYGKLAGRLTLADDSGIEVDALGGLPGIYSARYGGDGKN
                     DHDRNRLLLSNLNGIAEDELTARFRCAVALWNPIDDTVVTFEGKIEGRITRNAKGRNG
                     FGYDPLFFFPEKNRTLAELTSQEKESVSHRGEAMRKAITYMKENIFSLLED"
     gene            10226..11242
                     /gene="moaA"
                     /locus_tag="QF838_08735"
     CDS             10226..11242
                     /gene="moaA"
                     /locus_tag="QF838_08735"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HJO60803.1"
                     /translation="MGNIPIFDTFNRPLRDLRISVTDRCNFRCPYCMPAEIFGDRYEF
                     LPRADLLTFEEITRIVNIAVGLGVTKVRITGGEPLVRQNIEQLVGMIASVDGVEDLAM
                     TTNAYLLSGYAQKLKDAGLHRITVSLDSLDDDIFQKMNGRGFSTARVLDGIKAAKEAG
                     LNPIKINAVVQKGVNNHTVVGLAEWCRSNGHTPRFIEYMDVGTLNDWKLEQVVPANEI
                     VDIIRQRFPADPVGPTYPGEVAKRYRYKDGQGEFGVISSVSQPFCGDCSRMRLSPEGK
                     AVTCLFAESGTDIRKWLRNGSTDDEIRNTIIGIWGKREDRYSEERSEKTVKRKKVEMY
                     HIGG"
     gene            11232..11660
                     /locus_tag="QF838_08740"
     CDS             11232..11660
                     /locus_tag="QF838_08740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HJO60804.1"
                     /translation="MEDRNNLGEIPIVIYTDGACLGNPGPGGWGAVIFENGKKQQLHG
                     AEENTTNNRMEITAVLEALRTIPRNVDVRIFSDSTYVINTMTKNWKRKKNQDLWPQLD
                     NEVEARNVEWEWVKGHSGDRFNEEADQLAYREASRVAKER"
     gene            11692..>12027
                     /locus_tag="QF838_08745"
     CDS             11692..>12027
                     /locus_tag="QF838_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HJO60805.1"
                     /translation="MSTEKKLTHVNKAGAANMVDVGSKPNTERIAEAKAEVYMEEATI
                     ALIRDNSFDKGDVIATARIAGIMAAKETSKLIPLCHPLPISQVKVDIQLDEENKRVKI
                     ASIVKTNGKT"
BASE COUNT         3436 a         2541 c         2652 g         3398 t
ORIGIN      
        1 tggtaaatta cttagtggtg ttccgggatc tggcaattgc agcgctttta ttattggtgg
       61 gggaaccgtg ggatttaatg cagcgacggc gttgatgggg gcaggggctg acgtaaccgt
      121 tgctgatatc aatctagata gaattcggga aatagacacc ctaaccaatg gccacataaa
      181 aaccatttac ccgaccccag ccgcacttgc agagaagatc caacgctcag aggttgttgt
      241 gggtgcagtg ttagtgccgg gtgcaaaggc tcctaaatta gtcagtcgcg atatggttaa
      301 agatatgatg ccgggagggg tcattatcga tgttgcgatt gaccaaggcg gctgtgttga
      361 agacattcac ccaacctccc atcaaacccc gacctatatt caagacgacg ttatacatta
      421 cgcagtaacc aacatgccag gtatagtggg gcacactgct agtatttcgc tttcaaatgc
      481 cactttaccc tacgttcggt cgattgcaaa aaatggtgtc tacaaagctg tcactttaaa
      541 cagtagcctt gctaagggtg tcaacactat aggtggacaa ataacatatc ctgctctggc
      601 tgagagtctg agcatgacat tttcaaattt agaggaaatg ctaccttaat ttcatattaa
      661 gaacagggtc tgagataaga tgcaacctga tagacacata gtcactgcaa aggttgctaa
      721 catcaagcaa gaaaccacct ctacaaaaac ctttgatttg attccctata aaggcaaaat
      781 tgattttctt cctggtcaat ggattgatct gtttgtttcc ttaaacggga gggagcaggt
      841 ggctggttat tcaatcacat caaccccatg caatacagaa ttcatttctt tagccgtcaa
      901 aaacacaggg gatgatcaag taacccaata tattcatcaa tctatgctgg agggggatac
      961 tgtttctttt tcaaccggtg gaacctgtgt ttataatccc gagcagggta atgcgatatt
     1021 aatagccggt ggaatcggaa ttaccccttt ggtgagtatt ttcaggtaca tcggggaggc
     1081 aactaatcac agagcaacaa tcgtgcatag tgtttcatcc tctgaagagt tattgttcac
     1141 tagggaatta gaaaacatct gcctcttaaa acctgatcag ctttcttatg cctacacgat
     1201 atccagtgga tctcatcatt ccactaaagg gttctcaggt aggattagtg ccgaaatgtt
     1261 agatcaatta gacgtctcct cctttaatat cgcttttgtt tgtggtccag acagctttgt
     1321 ggattctctg tcagctcaat tacaaaaaac ggggtttaat aagggccaaa ttaaatcaga
     1381 gacttggtgg tagattccaa aacggcatgc ttatctattc atgccttcag taaatgtttt
     1441 ttactaaaag acccgaactc tttttttgtt aaagtatttc caatcaaact catatcccca
     1501 ccccggtctt tcagttaact ttacctttcc tttttccggt accggaggat ttagaaggcc
     1561 aatctcctta cctattgaat ctctactacc gtccggaaag tactcgtaat aagaagtatt
     1621 attaatgccc acggccaatt gggcatggac atgcccgaat aatcctcctg ggccatttag
     1681 ttcgatcttc ataccctttc tggctagatc ttctgcgatc cgaacaattc cggtagtacc
     1741 atggcgtgca tttccgcgaa ctatattgac cgcaccttct ctaacccatt ctctcattag
     1801 cacaaaatca ttcatcaaag tttcgagtgc caaaatcggc acctctgatt gtgaacaaag
     1861 tgttttcaat gaacccaatt ctctatcatt caacggctcc tcaaaccatc caaaattcag
     1921 ttggcttaat tgttttgcaa cctcgagagc ctcatgcagt gtatagctac aagcagccgc
     1981 gtcatgtaac aaagtaatgt cttctcccac aacacctttg acagattcga aagaatttat
     2041 gttttgtgct gcattccaag caaaatgatc ttttagaacc gtgaaccctt tttcgatacc
     2101 cacatttgca tcatcaatca acgcattaag atccgctccc cggtagttat agtaggccgt
     2161 gacctggtcg ttgctatcgg aaataagagc tgcaactgac ttgctcttga tttgcccctc
     2221 tatgtcccat aaacaattat caaacccgcc aaaccatcct gggggcataa aaaccattct
     2281 ggtcgcttta gtgagcatat caaacaagta ttcctttttt gttggatcct ccccaaccgt
     2341 aaggtgtttt agatgtgcaa gggcgtttac agtgattgtt gtataccgag cgtcccctac
     2401 cgtacagatt ccctccaacc cgtcatccgt tatcacatgc atcacatgca cgtagcctac
     2461 agaggatctg aggtcatatt tttcccatga actgtctatc ttacgaagtt tgaaatatgg
     2521 tgtgttgtcc ttgatttcaa attcactgat ttttatatct ttgattttca tatcgaactt
     2581 acatggatgc ttggttgata ggctttaatg caagcacatg gagtgctttt aagcggcgct
     2641 acctgttacg gcgaatcgat atcagatttc tctttatctt cttctttaga aaggtccttg
     2701 gtttcttcat cctgcttttg tcttgtctgg gccattcttc ctatgaattc aaaggcatcg
     2761 accgaatcca tttgtaagct cttgtcatcc tctggcatga gatgaatcct tttgaatgtt
     2821 ttgtggagcc gacggtcagg ttcgaactga cgacctgctg tttacaaaac agctgctcta
     2881 ccactgagct acgtcggcat aacgttttga attactgccc aaaatttagt gcagcgacaa
     2941 ccgttgtact aaacgacttc aacactattg tacaagactg atatgattcc attcccattt
     3001 ttcaaatatt atcgaacact taaattgcaa tacccatata tttaaataac ttagggaaga
     3061 gaatcaaact accatgagcg ttgaaatagt aatctatgga gcacaaatta taactggact
     3121 agccacttta gcggtagcct ttgttctggt cttccaattg aagcagcagc gttccgttgc
     3181 acaaagagaa ctggtgcttg caattaacca acaaagacaa gatctcgcgg tagccgtggc
     3241 taacaacact gaattatctg atataaattc ccgcggtagc catgatttcg cagatttgaa
     3301 taatcagtct gaaagaatcc gatttaacag attatttgcc gcagagatga gccttagcaa
     3361 catagcgcag gaatacgcgg acttattaca cgtggaccca gacttggcac taaggaccag
     3421 tttcgccctt ttccccggaa gaagaaaatt ctacaaggaa agcctgatta ggtttacact
     3481 acccagtgag tttgttgaaa aggttgatga acacataaaa gaaatagaag atagtgtagg
     3541 ggaagatggt caagacgtat ctgtgcttga cccagaaatg cgtaattctt aacttaggag
     3601 gccgaagatg gaacttttga ggtacctacc ggaaaataat atagagatag ggggaaaggt
     3661 tcctgatttc cttttagtag accatgaaaa caaaacctta cgactcagtg atttatctgg
     3721 ttctccggcc gtcctttatt tttaccctaa agatggaacc tcaggatgta cgcaagaagc
     3781 ctgcgatttc cgagacgtat acaccgattt ccttgaactc gattgcccag tttatggaat
     3841 aagccccgat accgaggcct ctcaccaggg tatggccaaa gagcacgatt tgccttttga
     3901 tttatgcgta gatccagatc taaataccat caaaggatat ggagtctggg gtgaattcca
     3961 attaaatgat agcgaggcgg agtatgccaa agggtttggg ttccacatca gtgaaaacct
     4021 tcgtgcttgg tctgtagttc gttgtacttt cctcatagac aagaatggga ttttagttca
     4081 caaatggatg aacgtgaatt ttagaggtca tgccagcagt gttagggatc aactccataa
     4141 atcgttcaaa ttcgacatag ctttctaaaa aatattgccc cgacacgttt attgaggtgc
     4201 tagtcccatt cccactccac atcaccaatt cggaccgtgt ctccttcggt aatccccgct
     4261 tcctctaagg cttttacgat tcccgttttc tgcatgtgtt tatagaattg catacgggca
     4321 agccaatcct cataatctat cctctgagct attctttcga ctcctggagc ttccacgata
     4381 tatattccat cttcttgatg taccgaaact cccctgcgcc gtggctgtgg gcgtagtatc
     4441 ggaatatttt tttcctcagg atcctcggag ggctctgggt ggtttgcgat aggttgatca
     4501 gataaaactt gaaccacctc atccatcatt tcggagacgc cctcgccaga tattgccgaa
     4561 actatatgaa acctccaggc atcttctccc attgagtccc tgacatcttc cgcgagtacc
     4621 cttacttcct caagatcaac cttgttgatc actaagattt gcggtttatc tttaagagac
     4681 tcgtcgaata attccaattc tttgttgatt ttgtgaaaat ccccacttgg gttatccgat
     4741 gttccatcta caagatgcac gagaacccgt gtcctttcaa tatgcctaag gaattcatgt
     4801 ccaaggccaa ttccttgatg ggccccttca agcaggccag ggatatctac catcacaaaa
     4861 tccttttctc ggtgttttac cagcccaaga accggttcaa tcgtcgtgaa agggtaactg
     4921 gcaaccttgg ggcgggcagc tgttagaaat gaaataaggc tcgatttacc ggcatttgga
     4981 acacctatta tccccacgtc agctatcaat ttcagttcca gtctcagatt gtgttcggac
     5041 cccaattcgc ccgattgggc gattactgga tattgactgg tggaagaaga gtatttaacg
     5101 tttccatacc cacctcttcc tcctgccgct gctatatatt cttctccgga gagtataata
     5161 tcagcgataa ttgagccccc ctcatcaatt atttgagtcc ccacaggaac ttttattata
     5221 gtgtcttttc cgtccttgcc atgcttcttt gctttagatc cattctcccc gtttccagct
     5281 atgaaatttt tgatatacct aaagttaata agggtgtttt cattgtggtc tccctttatg
     5341 atgacattgc cgcctctccc accatctccc ccgtcaggcc cccctctcgg tacaaatttt
     5401 tcatggcgac cgcttattgc tccgtcacct cctgacccgc ttcttatttg tattaataca
     5461 ttatcaatca taaattgatt ttacttcctt gtagataata aaaataataa taagggtgtg
     5521 taaatagtgg atatcaatat ctggtagtct gtggatatat atagatttta tcatgatgta
     5581 ttgtggaaga tcggaggagg aataaaaata tttgttaata gtatcaatgg tttatgtgga
     5641 gtgtggatat cgatggtgtt ttgtcccctg cttgccctct tgttattaat aaaatagtca
     5701 tataattgca caggctttcc acgagttctg tggaaacttt tttagataca ggaacattta
     5761 tattgttgag gagataaatg gaagagatgg caccttatat taaaaacaaa acaaccgtta
     5821 gtgaaatgtt cagcgaagct aacctttttc agtcctattt agatgttgaa gcagccctgg
     5881 caagagctca ggcaagccaa ggaattatcc ctgccaaagc tgccgagata atatcggaaa
     5941 aggccaaggt tgagttctta aatctacaaa atatttacga tgggttagat aagacaggac
     6001 acccgctggt tcctttaatt tgggagttag accggatatg tggtgaggag gccgggggat
     6061 atatacattg gggggccact actcaaaata taactcagac aggaaaactt ctattaataa
     6121 aaaagtgtca caatagttat ctagaagatc tcggcagctt gcttcatata cttggacaac
     6181 tggctgaaac gactaaagat tatgctatgc caggaagaac acatggccaa catgctgtac
     6241 ctgccacctt tggttacaaa gttgctgtat ggatagatga attgattcgg catgttgatc
     6301 ggttgcaagc aagcgagcgt cgtgtttttg tggcaatgct tgggggaggc gcgggaacta
     6361 tggcttctgt gggtcttgaa gggcttgaaa cccaaaggct gataggaata gaacttggat
     6421 tagggtccat gacgatgccc tctagagtca taggagacca tctgacggaa tacatcacta
     6481 ttctagccat gctcgcagct acttccacca aaatggctcg agaaatctac accatgatga
     6541 agcaggagtt tggtgaagtt gaagagcctg ttcccgtagg ggcggttggt tcttcaacta
     6601 tgcctcagaa aaggaacccc cggctatgtc aagatgtagt cgcttgggct tctgaaatca
     6661 ggacatttgt gccaatgtcc ctagaagcga tgcaaactga acacgaagcg gataaaacca
     6721 ccagtatgat gataaattct gctatcgata ggacgtgcgt cctaatgggt cagattacaa
     6781 aaggtctcgt tgaaatattc acggacctaa aggtttttcc agatagaatg cggcaaaatc
     6841 tcgatcttag tggagggtta atactttcag agagaatcat gctggaactt ggtcgaaaaa
     6901 tgggtcgaca aaaggcgcat gatgcggttt atgaggcagc ccaaagagcc gtcaatgaag
     6961 ggaggccatt ttctcagacc ttgcaggagg aaccagaggt ggccagccag ttaaccaagg
     7021 aggaaatact aaggctattg gacccagaag aatatacagg tctgtgttcc tactttgcag
     7081 aggagtttgc attaaaagct gagcaaaaaa gcaaggaatt aatcgataat taacacacta
     7141 tatctttatc ggtaaaacct cagggatagc gcctgtttct cttaggcgag caatctcttc
     7201 tgttgttaat cctagttcct ttaatatagg cacggtatcc cctccaaaaa tagggggatt
     7261 aacatcttgt aaaggagtct cagacataat gacgggattt cccgcccaag aagtgttgcc
     7321 ccatgaggaa tttagatcca tcgaattcgt atagtcctta tctttgataa gacctcgctc
     7381 gtgattgaat tcatcaccat gtatttcagc tgctgttgta tttttcacca aaggaattct
     7441 aatggagctc atggtctcaa tccaatatgt ggtggaatta gcgacaaaga tcttttcaaa
     7501 ggcttcacgt aaaaggagat catttttcga ccttaaatca tcagataaaa actcttcctg
     7561 taaaccaagg tttgaaaact cctctatcct gcaaagtgtt tcccattccc tgttagaaga
     7621 aatacttaaa aatacccaac tgtctgagga ctcgtacaac ccagttaatg catttaaccc
     7681 gcgaactcct ggtccgccga tatcattcct cacgtaattt tggtgcgtga catgatactg
     7741 gccagatatt gtggccgagg tataagacaa agctgcttca acctgctggc cccgcccagt
     7801 tttttctctt tcgagaaggg ccatcattat cccgtatgct gcggcaatac cagttccgta
     7861 gtcatttaaa gtaaatgtgg ccggccttgg ctgccctttg ccaccgctat tgcgtagctg
     7921 aacccctgtg gctgcctggg cgttttgctc gaaacccggt ctaaattccc aagggcccga
     7981 tcggccgaaa cagttaatag atgcgtagac aaggttaggg tttctggcct tgagttgttg
     8041 gtagcctatc cccaaccgat ccgttacccc cttcctaaag ccttctaaaa tgacgtcggc
     8101 tgaatcgact aatttgagaa atgtttctaa tccgtctggg ttcacgatgt ttaaagagat
     8161 actccgtttg cctctaccaa cgtctaacca aggagtcaat tgaggcgggc gatgttcggg
     8221 gtcaattttt attacctcgg caccatattg tgcgagtgtt tttccacagg ttggcccggc
     8281 tagcacaatg cataaatcca aaactcggat accgtctaga ggtagcttag tcatcgattc
     8341 acctcaggtt gaggcttttc ggtttgtgaa gctctggcag aggctaaagt tccatgatca
     8401 tggttataaa gacggacaat ttttcctgcc tgtttcattt ttccgaaagt agggtcctca
     8461 atagtgacag tggcgcctga ggccatggag tgttcagaat ccaaccattc gtctatagtc
     8521 cggcacattg ttcctggaat gcccaattcg tccatgatgt tttcccactc caaggcgggt
     8581 ttatttttcc atatcttagt aaattggttg atccagcggt tccggtctgt ttcattagat
     8641 aataggttgt ttatatcagt gagcggttct agccattcag tatggccaat attgtccaga
     8701 aacggtttta agaacctaga gaaagagatg ttaacgttaa tccaccgttt gtctttgcac
     8761 tggtaaaatc tttttataac aggatgacct tcctggtttc cggtgcttct ttttgaatgt
     8821 cggactaggg ctgttcccat tccttgatac atagcatcat ggacaggaac ggtaatttgc
     8881 tggccagtgc cctgtttttt cctgtggtac agtgcagcca caacggctgt ggaagctgtt
     8941 atggctgcaa atgtactggc gtaaggcaac ccatggaacg atggcccttc tccggcaacc
     9001 ggaaattcgc ctgatggatt gaaagaatag atccctgagg ctgaggcaac gattccttcg
     9061 tctggaggaa ggtcttcata gatatgtcct tttggaaacc cgggcaagga aacacatact
     9121 agacctggat tatcttttgc cagagagtta taagatagat taaagccttt gtagcagtca
     9181 gggtggttat cgtaaataat tacatcggcg ccggcggtat gtttaataaa ctcttcttgc
     9241 ccttggtgat cactcacatt tatttcgagg gactgtttcc ctctgttcca gattgagatt
     9301 gcgtgacctt caaaccgcat aggataatct ttcggagaat caattctaac cacctcagcc
     9361 ccctggtcag acaatagcat gcctaaaatg gaacctacag ggtacgaact aaactcaata
     9421 actctatatc cagttagggg acctatccta ttcactcctt aaatattggg ccaatatgtg
     9481 gcaaataagc ctaaaatcaa attatacagg aggtgagaat tctctgaata agtacctgag
     9541 gtaacttatc ctaatgaata aaaacaccaa tcgaatataa ttaatctatg aaaaatacta
     9601 tcaacctgcc aattgaaata gtgatcgcaa cgcgtaattc aggaaagatg gatgagatgt
     9661 cagaaatgtt gtccaacagc gcctataagc ctctttctct tgaacaagtc cgagtggttg
     9721 aagaagtgga ggaaacagga acaactttca aagaaaatgc tattttgaaa gccgaaacct
     9781 atggcaaatt agccggtaga ttaactctgg cggatgattc cggaatagaa gtagatgcgt
     9841 taggggggtt acctggaatt tattcggccc gatatggcgg tgacggcaag aatgaccatg
     9901 ataggaatcg actgttgtta tcaaatctaa atggaatagc agaagacgaa ctaacagcta
     9961 ggtttcgatg tgctgttgct ctatggaacc ctattgatga tactgttgtg acatttgagg
    10021 gcaaaattga gggcaggatt accaggaatg cgaaaggaag gaatggattt ggatatgatc
    10081 ccctattttt ctttcctgaa aaaaacagga ccttggcaga attgacctca caagaaaaag
    10141 aatccgttag ccacagggga gaagctatga gaaaggcgat aacttatatg aaagaaaata
    10201 tttttagtct ccttgaagac taaaaatggg aaacatccca atatttgaca cattcaaccg
    10261 accactgcga gatttaagaa tctctgttac ggatcgttgt aactttcgat gtccgtactg
    10321 tatgccggca gaaatttttg gagatcggta tgaatttctt ccccgggcag atctgctaac
    10381 ttttgaggaa attactcgca ttgtaaatat tgctgttgga ctaggggtca caaaggtgag
    10441 aatcactgga ggggagcctt tagtacgtca aaacatagag caattggtag gtatgattgc
    10501 atctgtggat ggtgtcgaag atttagctat gacgacaaat gcctatttgc tatctgggta
    10561 tgcacaaaaa ttgaaagacg cagggttgca ccggattacc gttagccttg attccctcga
    10621 cgacgacatt tttcagaaga tgaatggaag aggatttagc actgctcggg ttttagatgg
    10681 aataaaggca gcgaaagaag cagggttgaa cccaattaaa attaacgctg ttgtacaaaa
    10741 gggagtcaac aatcacactg ttgtggggtt ggcagagtgg tgcaggagta atgggcacac
    10801 tcctcggttt atcgaatata tggatgttgg gacgttaaat gattggaagc tagagcaggt
    10861 tgttcctgct aacgaaattg ttgacataat aagacaaaga tttcctgctg atccggtagg
    10921 tcctacttac cctggggaag tcgccaaaag atatcgctat aaagatgggc aaggagaatt
    10981 tggtgttatc tcttcagtat cccagccatt ttgtggagac tgctcacgga tgcgactgtc
    11041 tccggaagga aaagcggtca cctgcctttt tgcagaatca gggactgata taaggaaatg
    11101 gttacgaaac ggatctactg atgatgaaat cagaaacacc atcattggga tctggggtaa
    11161 gagagaggat cgttattctg aagaacgatc tgaaaaaaca gtaaaaagaa aaaaagtgga
    11221 gatgtatcac attggaggat agaaataact taggagaaat tccaatagtt atatataccg
    11281 atggtgcatg cctggggaac cctggtcctg gcggctgggg ggccgtcatt tttgaaaatg
    11341 gaaaaaaaca acaacttcat ggagcagaag aaaacaccac caataaccga atggagatta
    11401 cggcggtgct tgaggcttta agaaccattc ctagaaatgt cgatgtgaga attttttctg
    11461 attcaaccta cgtcataaat accatgacta agaattggaa gagaaagaaa aatcaagatt
    11521 tatggcctca gttagataac gaggtagagg ctaggaacgt tgaatgggaa tgggtgaaag
    11581 gtcattctgg tgatcgtttt aatgaagaag cggaccaatt ggcgtatagg gaagcctcaa
    11641 gggtggctaa agaaagatag cagcataata tttacatcag aggagccaaa agtgtctaca
    11701 gaaaaaaaat taactcatgt taataaagcc ggcgctgcca atatggttga tgtaggatca
    11761 aagccaaaca cagagagaat tgccgaggcg aaagccgagg tatatatgga ggaagcaacc
    11821 atagctctga ttcgggacaa ttcttttgat aaaggagatg tgatagccac ggctcgtatc
    11881 gctggcataa tggctgctaa ggaaacctct aaattgatac cactatgtca tcccttacca
    11941 atcagccagg taaaggtaga tattcaattg gatgaagaaa ataagagggt taaaatcgcc
    12001 tctatcgtta aaaccaatgg aaaaact
//
LOCUS       DASCNS010000243         5758 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001855393, whole genome shotgun sequence.
ACCESSION   DASCNS010000243 DASCNS010000000
VERSION     DASCNS010000243.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001855393"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="QF838_08750"
     CDS             complement(<1..750)
                     /locus_tag="QF838_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014759.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HJO60806.1"
                     /translation="MNPNPTDTYDNKSLLILIDGHALIHRAFHAIPQPLTVSTSGEDV
                     RGVYGFTNAFIRALSELKPTHVAITFDLSAPTFRHKMFDEYKAHRPPTPPELRTQFDR
                     VRQVMSTFEVPIYELEGFEADDLLGTLSHQAEETNLSTVILTGDSDTLQLVSGTTRVF
                     MNSSFQKSSIYDINAVKERYGGLGPEFVSEIKALQGDSSDNIPGIPGIGIKTAIKLLS
                     DFGSIQGIYDHLEEVTPPRAKKNLAENKAIAD"
     gene            complement(791..1888)
                     /locus_tag="QF838_08755"
     CDS             complement(791..1888)
                     /locus_tag="QF838_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HJO60807.1"
                     /translation="MLDRFKVPKEDQVKVSEPALRKTVEDIFEKTGLSKEDAKNGTDV
                     LVTADLRGVETHGVSNMLRVYVQLFEDGDLNKTPNIKVVKESPGTATIDADRALGIMA
                     GIQAMEMAISKARNVGVGVVTMFNSGHMGAVGHFSMMAAQKDMVGMCSVAAGTGILPT
                     FGAEPRLGTNPISIASPANLEAPLLFDAATSAVAGNKLRLAARVDATLLPGWVASSDG
                     SPIMEETKIVDRDDYYQLPLGGSREQGSHKGYGFGLMVEILGSLLSGDDPGMLRDTPS
                     WTPSRTYFAAYNVDAFTDIDDYKKNMDDMLATLKSTPPAPGHDRVLYPGLSESEEMAK
                     RKVGGIPLHKEVIGWFSETTQRLGLKELEVL"
     gene            complement(1960..3381)
                     /locus_tag="QF838_08760"
     CDS             complement(1960..3381)
                     /locus_tag="QF838_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014877.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HJO60808.1"
                     /translation="MTIRFGTDGWRALMTEEFTVGNVKICAQAVCNFMKDRDVWGNGL
                     IIGYDTRLNSNIFAETVAEVAAGNQIPVILCDRPCPTPVLSYNLVSRECGAGVVITAS
                     HNSREWNGFKFKPSYGGSASEAIISEIEKYIFEVEQSQNISTMPIETAEQIGLLEKLD
                     PEPTYLNHIAELVDLNRIRNAGLNIAIDSMHGAGSKYFAKLIEGGSSRVAEIRGNPDP
                     NFPGMIQPEPIEKNLLPLKELLSDSNFDVGLATDGDADRLGLTDEDGNYISTLHTFAL
                     ICKHLLENLGLKGPIIKSITMTNMINKLGELHDIDVIDTPVGFKYLGPVMMEENAIAG
                     GEESGGYGFTGHIPERDGLLSGLMILDMMSVTGLNPSDLLEDLKDLTGPHHFERLDIA
                     FNEQDREDKLLRISTSTFEHVGELEVLSTDTRDGLKLHLEENAWALIRFSGTEPLIRL
                     YAEGSSPDQVKNIIENCRMIVGS"
     gene            complement(3400..4614)
                     /gene="metK"
                     /locus_tag="QF838_08765"
                     /pseudo
     CDS             complement(3400..4614)
                     /gene="metK"
                     /locus_tag="QF838_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955270.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            complement(4655..>5758)
                     /locus_tag="QF838_08770"
     CDS             complement(4655..>5758)
                     /locus_tag="QF838_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HJO60809.1"
                     /translation="VTTETANLAITLRDGGADVFLCASNPLSTQDDVAAALVSEYGIP
                     TYAIKGEDTDTYYEHIISVLNTKPHMTMDDGADLVATLHGDRTDLMENIIGGTEETTT
                     GVIRLKSLAEEGNLKYPVIAVNDADTKHFFDNRYGTGQSTLDGITRATNVLWAGKTVV
                     VIGYGWCGRGVAMRARGMGGNTVVCEVDSIRALEAAMDGHQVMKMDEASKIGDIFITV
                     TGGMKAIDEKHVANMKDGVLLANSGHFNVEIDIEGINELSDSKSEVKPFVEQYILTNG
                     NRINLLAEGRLINLAAAEGHPSAVMDMSFADQSLSAEFVAKEYKNLENQVYVVPREID
                     NEVGRLKLLSMGIAIDELTVEQEKYLNSWNVGT"
BASE COUNT         1451 a         1381 c         1097 g         1829 t
ORIGIN      
        1 atcagctatt gctttatttt ctgctaaatt tttttttgct cttggagggg tcacttcttc
       61 taagtggtcg tatatgcctt ggatagatcc aaaatctgat agcagcttta tagcagtttt
      121 tatcccaatt ccaggtatcc ctgggatatt gtcagaagaa tctccctgta atgcttttat
      181 ttcagacaca aattctggtc caagtccccc atatctttct ttaacggcgt ttatgtcata
      241 gatagacgat ttttggaaac tcgaattcat gaaaactcta gtcgtgccag atactaattg
      301 cagggtgtca gaatctcccg ttagaataac tgtactgaga tttgtctcct ctgcctgatg
      361 agacaaagtt cctaaaaggt catctgcttc gaacccctcc aactcataaa taggcacttc
      421 aaaggtagac ataacctgcc tgaccctgtc gaattgggtg cggagttccg ggggtgtggg
      481 agggcgatgg gctttatatt cgtcaaacat tttatgtctg aaggtaggag ctgagaggtc
      541 aaaagttata gccacgtggg taggctttaa ttccgataaa gccctgataa aagcattcgt
      601 gaatccatat acacccctga cgtcctctcc actggtacta acggtcaaag gctgtggtat
      661 cgcatgaaat gcccgatgaa ttaaggcgtg cccatcaatg agtatcagaa gagatttgtt
      721 gtcataggta tccgtaggat ttgggttcat ggtagaagaa cgggtccctt tttattcaaa
      781 ttttggtcat ttatagcact tctaattctt tcaatcccaa cctctgtgtg gtttcggaaa
      841 accatccaat aacttccttg tgaaggggta tcccacccac ttttcttttg gccatctctt
      901 cgctttcaga cagtccggga tataaaaccc tgtcatggcc tggtgcaggt ggtgttgatt
      961 taagagttgc gagcatgtca tccatattct ttttgtaatc atcaatatcg gtaaatgcgt
     1021 ctacgttata tgctgcgaaa taggttcttg aaggtgtcca agagggtgta tctctcaaca
     1081 taccaggatc gtccccagaa agaagtgacc cgagtatttc caccattaat ccgaaaccat
     1141 atcctttgtg cgaaccctgt tctcttgatc ctccaagagg gagttgataa taatcgtctc
     1201 tatcaactat tttagtttct tccattattg gggaaccatc gctactcgca acccagcctg
     1261 gtaaaagtgt ggcatccact cttgcagcca atcttaattt gttccctgca acggccgaag
     1321 tggctgcatc gaacagtagg ggtgcctcta ggttagcagg agatgctatg gaaatgggat
     1381 tggttcctaa tcttggctct gcaccgaatg ttgggagaat tcctgtcccg gctgccactg
     1441 aacacatgcc caccatatct ttctgggcag ccatcattga aaaatgtccg accgctccca
     1501 tgtgaccact attaaacatc gtaaccactc ctacgcccac gtttcgtgct tttgaaatgg
     1561 ccatttccat ggcttggatg ccggccatga ttccaagtgc cctatcagcg tctattgtgg
     1621 cggtacccgg actttctttg actaccttta tatttggggt tttgttgagg tctccatcct
     1681 caaaaagttg cacataaact cttagcatgt ttgagacacc atgagtttca actcctctta
     1741 ggtcggcagt gaccagcacg tcagtcccat ttttggcatc ttccttgctt aatcctgttt
     1801 tctcaaatat atcttcaaca gtctttctca aagcaggctc ggatactttt acttggtctt
     1861 ccttgggtac tttaaatcta tctaacacta caacctccaa agtctcttca gaattgcgga
     1921 cataaagctc aaattaaatt ctattagttt aggtaccttt caactgccga ctatcatacg
     1981 gcaattttcg atgatgtttt ttacctggtc tgggctactt ccctcagcat atagcctgat
     2041 taatggctca gtacctgaga atctaataag ggcccatgca ttttcttcaa ggtgcagttt
     2101 gagtccatct ctggtgtcgg tgcttaagac ctccaactcc cctacatgtt caaatgttga
     2161 cgtgcttatt ctaagtaatt tgtcttcacg atcttgttca ttaaatgcta tgtccagtcg
     2221 ctcaaaatga tgtgggcctg ttaaatcctt caaatcttcc aacagatctg aaggatttaa
     2281 gcccgtaacg gacatcatgt ctaatatcat taggccagaa agtaaaccat ctctttctgg
     2341 aatatgacct gtaaatccat atcccccact ttcctctcca ccggctatgg cattctcctc
     2401 catcatcact gggcccaaat atttaaaccc cacgggagta tcaatcacat ctatatcatg
     2461 taattcgcct aatttattta tcatgttagt catcgttatc gattttatta ttggcccctt
     2521 caatcccaga ttttcaagga gatgcttgca aattagggca aaagtgtgaa gtgtcgagat
     2581 gtaattaccg tcttcgtctg taaggcccaa acgatcggca tctccgtcag ttgctaagcc
     2641 cacatcaaaa ttcgagtcag aaagaagttc ttttaagggt aggaggtttt tttctatagg
     2701 ttccggttga atcatgccag ggaagttggg atctggattt cccctaattt ccgctacccg
     2761 acttgatcca ccctcgatca gtttggcgaa atacttggat cctgcgccat gcatggaatc
     2821 tattgctata ttaaggccag catttctaat cctgtttaag tcaactagtt ccgctatgtg
     2881 gtttaaatag gttggttcag ggtctaattt ctctaacagt cctatttgct ctgcagtctc
     2941 aataggcatt gttgaaatat tttgagattg ttctacctca aatatgtatt tctcaatctc
     3001 cgagattatt gcttcggaag cgcttccacc gtatgacggt ttaaatttga aaccattcca
     3061 ctcccttgaa ttatgactcg ctgttattac caccccagcg ccacattctc gtgacacaag
     3121 attgtaactt agtacagggg ttgggcaggg ccgatcgcat agaattacag ggatttggtt
     3181 acctgctgca acttctgcca cggtttccgc gaaaatattg gaatttagtc ttgtgtcata
     3241 tccaattatt agaccgtttc cccatacgtc acggtctttc atgaaattac aaaccgcttg
     3301 agcgcatatc ttcacatttc cgacagtgaa ctcttctgtc atcagggccc gccagccatc
     3361 tgtgccaaat cttatagtca agaccccccc taaaaattat taaaagctta tggctttttt
     3421 tatagcttca actttattag ttttctccca cggcaagtcg atgtcagttc ttccgaagtg
     3481 tccatatgag gctgtctggg catatatcgg ccgtctgagg tctaagtccc ttattattgc
     3541 ccctggtctc aaatcaaaat gtgtttctac tatttgatcg atttgatggt ttgaaatttt
     3601 gtttgtacca aaagtttcaa ctgagactga tattggagct gatattccga ttgcatagga
     3661 tacttggact tcagctctac tcgcgatacc tgctgccact aaattttttg cgacccatct
     3721 cgctgcataa gctgcagacc tgtcgacctt tgttgggtcc ttaccggaaa atgcaccgcc
     3781 cccatgtctt gccattccac cataagtgtc aaccaaaatt tttctacctg tcaagccagt
     3841 atctccgaca ggtccaccta taacaaacct cccggatgga tttacaatga atcgggtgtc
     3901 aataagtaaa tctttaggaa ttatatcctt ggccaccttt tcgatgaggt ctttttcaat
     3961 gatttcgttt gctacatcag gatcatgttg attactaagt agaacagtat gaacacgttt
     4021 gggtctgccc ttggcatatt caaccgtaac ttgggatttc ccatctggac ggaggtattt
     4081 taactccccg cttttcctta cttcggatag tttttttgtt aattgatgtg aaagagccat
     4141 tggtaatggc atcaattctg gagtttcgtc acacgcaaac ccgaccatca ttccttggtc
     4201 tcctgctccc aatgattcga cctcatcatc tgacatgtct tttgactacc tggtttccaa
     4261 agcgttgcta acacctttag agatatcaga tgattgctca tcaatcgcta ccatcacccc
     4321 gcaggtttga tagtcaaaac cgtaggctgg gtctgtatat ccaatactct taattgtgtt
     4381 tctaaccaat ccaggaatat ccacatagcc ttcagatata cttatttctc ccatcactac
     4441 caccagccct gtggtgcaag agacttcaca tgccactctt gctctggggt ctaatttaag
     4501 catagcgtct aggacgccgt cagaaatctg gtcacatatc ttgtcaggat gtccttcagt
     4561 aactgattct gaagtgaata ggtacgattc agattccatg aaatttaagg tcattattta
     4621 atctccaaat gataggctgg gaatttgatc ttaattaggt accaacgttc cagctgttta
     4681 ggtatttttc ttgctctact gtgagttcat caattgctat ccccatcgat agtaatttta
     4741 gtcttcccac ttcattatca atttctcttg gtaccacata aacttgattt tccagatttt
     4801 tatactcttt ggctacaaac tccgccgaca gcgactggtc tgcgaaactc atatccataa
     4861 cggcggaagg gtgaccttct gctgcagcca agttgataag gcgcccttca gctagtaaat
     4921 ttattcggtt gccattggtt aggatgtatt gttctacaaa cggtttaact tcacttttac
     4981 tatccgaaag ctcattaatt ccttcaatat cgatttcaac attgaagtgc ccgctattag
     5041 ccagtaatac tccatctttc atattcgcaa catgtttttc atcaattgcc ttcataccac
     5101 cagttacggt aataaaaatg tcacctattt tagatgcttc gtccatcttc atgacctgat
     5161 gaccgtccat tgctgcttcg agggctctta tggaatcaac ttcacagact actgtattac
     5221 cacccatacc cctagccctc atagctactc cgcggccaca ccatccgtaa cctataacca
     5281 caacggtttt tcctgcccaa aggacgtttg tcgcccttgt tataccatct aaagtacttt
     5341 ggccagtacc ataacggttg tcaaagaaat gcttagtatc agcatcatta actgcgatta
     5401 ccgggtattt aagattacct tcctcggcta gactttttaa cctaattacc cctgttgtag
     5461 tctcttcagt gccaccgata atattttcca tcaagtcagt acgatcaccg tgtaaggtag
     5521 ctaccaaatc agctccgtca tccatagtca tgtgcggttt ggtatttagg accgagataa
     5581 tatgctcata ataagtgtca gtgtcctccc cttttatagc gtaggtaggg atcccatact
     5641 ctgacactaa agcggccgcc acgtcatctt gagtactcaa agggttactt gcgcacaaaa
     5701 atacgtccgc accaccatct cgcaaggtta tcgccaaatt tgcagtctca gtagttac
//
LOCUS       DASCNS010000244         7392 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001857675, whole genome shotgun sequence.
ACCESSION   DASCNS010000244 DASCNS010000000
VERSION     DASCNS010000244.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001857675"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="QF838_08775"
     CDS             <1..419
                     /locus_tag="QF838_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60810.1"
                     /translation="YLYLFITIFILVFILPWFTMIWNPVRRSVWGPPILAASVLLGTL
                     LDRIRLYVASWDVAGKNMVSQLEMEMREVPTKIPSPEYADIFIMVGFIGGSILTFMLA
                     TRLIPAVNLWEQKEMLLYKAEVQFHRTKVMVMGKPR"
     gene            482..1891
                     /gene="nrfD"
                     /locus_tag="QF838_08780"
     CDS             482..1891
                     /gene="nrfD"
                     /locus_tag="QF838_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HJO60811.1"
                     /translation="MQEHTQLSHKQINEDLLKGVLTVPKWWLPAVAFLSLVVLTGLGA
                     FFYMVYHGLGVTGLNRPVMWGIFIANFVFWIGISHAGIMISAILRLTQAEWRRPITRA
                     AEVMTVFSLIAALHTPLMHVGRPWRVLYWIFPYDFSRGIWPNIRSPFVWDPSAVGTYL
                     IASSLFVFVCLLPDIAILRDRAKKPIVKKIYAQMALGFRGTPRQWKLQIIAGILLSAL
                     VLPVFVSVHSIVSWDFAVLIGVEGWHSTIFAPYFIIGAIHSGVSAVAMLMALSVWLYG
                     LDKYIKPDHFDAIARLLIIVATTWFFFFFLEWVYALYTLESPEIALRELQAFQWPYGP
                     LFAIFIITSFVIPVPMWLFKRVRRSAVLMFWTTILVNIGMWLERFLIVIPGLVRRTHM
                     TFDWGTYHPSIVEILVVVETFAFVCLGMLLFAKVLPLIPLFDIKEGMVNRRLIKVGRR
                     VIPATIREGLPHHYYEKKH"
     gene            1916..2431
                     /locus_tag="QF838_08785"
     CDS             1916..2431
                     /locus_tag="QF838_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HJO60812.1"
                     /translation="MAKRSVLGLYTEEDSAADALDNLREAGFEEGEYEILTGVPYPEG
                     TFGEAEPEHKLYRWPLIGAACGFTIALLITAGTELAYPLITGGKPILSIPAMAIIMYE
                     GSMLGAIIFTIIGIIFESRLPRIFMGAYSEKITEGHIGVTVTTDETRTDVAETALNKA
                     GTDGEIIRGWK"
     gene            2449..3111
                     /locus_tag="QF838_08790"
     CDS             2449..3111
                     /locus_tag="QF838_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024834.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HJO60813.1"
                     /translation="MSNNGLNIKITAPIVMVAILLVTLLVNSGCYNSKTGEVKVGGSI
                     SFELPVFPETGSHAVQIFSEMQYSPSYRSQEVPRLLPPEGSVPVTGAEVKILEVEALK
                     ALSMPDHDGSQAAKIYSVNCQVCHGQSLRGDGPITTLSSARGDGSKAYSGAAPVNLIS
                     ERVNDLSDGEIFGYITWGGRPGLAAAARGKDSTSIMPQFGYLLNEEQRWQLVQYIRDQ
                     NK"
     gene            complement(3143..3529)
                     /gene="acpS"
                     /locus_tag="QF838_08795"
     CDS             complement(3143..3529)
                     /gene="acpS"
                     /locus_tag="QF838_08795"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HJO60814.1"
                     /translation="MLITGIDIIEIARVGEVLNKYGARFLNRIYTDSEQRYCKGRAAQ
                     LASRFAAKEAVMKALGTGIRGVGWKDIEIKRERGGPPYIQLHGRGQARASIMGLSEIS
                     LSLSHSNDFAVASVVGEAKRGNRLSK"
     gene            3623..4504
                     /locus_tag="QF838_08800"
     CDS             3623..4504
                     /locus_tag="QF838_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HJO60815.1"
                     /translation="MKYKLIDGKAISESIKLEVKMSVRRLYEESSMIPSLSVILVGDD
                     PASSVYVRNKKSSAINLGMESQIINFPEVVTKEELLRQIRILNQDDNCDGILIQLPLP
                     AHLDENDILCTIDPQKDVDGLHPQNVGLLSMGAGSLIPATPAGIREMLLRSENDPKGK
                     HVVILGRSNIVGKPLSNLLSQKGHGGDATVTLCHSMTNNLKDITKSADILVAAIGIPN
                     FVTSDLVSSGVVAIDVGVNRIEDSTRSRGYRLTGDIDFEGVSRKASAITPVPGGVGPM
                     TIAMLLKNTLKAVLRKI"
     gene            4637..5779
                     /gene="dnaJ"
                     /locus_tag="QF838_08805"
     CDS             4637..5779
                     /gene="dnaJ"
                     /locus_tag="QF838_08805"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HJO60816.1"
                     /translation="MTTHKRDYYEVLGVSRNSTDDDLKKAFRKLAFKYHPDRNKEPEA
                     EEKFKEINEAYQILSDKEKRAQYDRFGHAGVGSSPTGANGSSGFGDVSGFGDIFDSFF
                     GGSGFGRSSRAQNSARRGSDLQYTMTVEFEEAIFGAEREFQIHRTESCSRCRGSKSEP
                     GSSVATCGTCQGSGQVRKAQQSVFGQFVQVGDCNNCKGSGKIVSNPCSSCNGQGRERK
                     NRKLAITIPAGIEEDTQVRLTGEGEHGINGGPPGDLYVAFKIKEHDYFVRDGINIKYR
                     LPINIVRASLGTKIKVPTLDGDYEIDIPPGTQTGRIFRVRGRGVAQLRGDRRGDLLVL
                     IDVRIPKKLDSQQQKLMNELGESLPDTFEDDEDKGIFDKFRSAFTN"
     gene            5776..6693
                     /gene="prmA"
                     /locus_tag="QF838_08810"
     CDS             5776..6693
                     /gene="prmA"
                     /locus_tag="QF838_08810"
                     /inference="COORDINATES: protein motif:HMM:NF018076.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HJO60817.1"
                     /translation="MKWLELSVEAPPEYVEPLTHIFYRYGEGGVAIESPAEFNPDEGE
                     RAPQPQTLKVVTFVPLDESARLRKSNIEIAVRLVAHFASISEIKEREVEEEDWESNWK
                     EYFHPIRVGSNLVICPTWRKHQSLDDDVLIKLDPGMAFGTGHHPTTRMCMELLETLIV
                     GGEKVIDVGCGSGILSISAAKLGAGDILGIEVESRAVEVALENCELNGISPQITILNG
                     TFPEIRVESASFDVALANIAAKVVINLSEYIVGAVANGGRLVLSGILKNSLKDVEKEY
                     SLHEVHFDKVLVDGDWTAVLATKKIATDM"
     gene            6701..>7392
                     /locus_tag="QF838_08815"
     CDS             6701..>7392
                     /locus_tag="QF838_08815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HJO60818.1"
                     /translation="MNWVVMHRFFIDPSDIQDGRVQFPEETSLQMKKVLRLRSGNEVV
                     VFDGGGVDYQVRLEVVEAKASFGTVLSTNDSNNEPAIFITLYQALIRSERYEYALQKG
                     VEAGISRFVPFTSERTQVSTPSHSKMGRWRRIIKEAAEQSGRSVVPKLADPILFSNMI
                     KNLNSVALIPWEQEKNQSIKQVLSRSHQDPGLNVTSIDLIIGPIGGFTPQEVSDAVDC
                     GATSVSLGKRIL"
BASE COUNT         2084 a         1318 c         1814 g         2176 t
ORIGIN      
        1 cgtatcttta tttgttcatc acaatattta tattggtatt tatcctgccg tggttcacga
       61 tgatttggaa tccggtaaga agaagcgtct ggggaccgcc tattttggcg gcctcagttt
      121 tactaggtac ccttcttgat aggataaggt tgtatgtcgc atcttgggat gttgctggta
      181 aaaatatggt aagccaactt gaaatggaaa tgcgtgaggt gcctaccaag attccctctc
      241 cggaatacgc agatattttt ataatggtcg ggtttatagg tggctcaatc ttaactttta
      301 tgttggccac taggttgata cctgcggtca atttatggga acaaaaggaa atgcttcttt
      361 acaaggcgga agtccagttc cacagaacta aagttatggt aatgggcaaa ccaagatagt
      421 aattgaagcc aggcgaggga atttagtcat tttgaaactt aaattagatg gatatattta
      481 aatgcaggaa catacacaat taagtcataa acagattaac gaagatctgt taaagggcgt
      541 cttgactgtg cctaagtggt ggttgcctgc ggtcgcgttt cttagtctag ttgtacttac
      601 cgggttaggt gctttctttt atatggtgta tcacggattg ggcgtgactg gcttaaacag
      661 gccggttatg tgggggattt tcattgccaa ttttgttttc tggattggaa ttagccatgc
      721 aggaattatg atatctgcga ttctgagatt gacccaggct gaatggcgcc gtcctattac
      781 aagagcggca gaagttatga ctgtattttc cttaatcgca gcactgcata ctcctttaat
      841 gcacgtgggt cgaccttggc gtgtcttgta ctggattttc ccgtatgact ttagtcgtgg
      901 tatatggcca aatatcaggt ctccattcgt gtgggaccca agtgctgttg gcacatatct
      961 gattgctagt tctctgtttg tgtttgtttg tttactacct gatatagcga tacttagaga
     1021 tagggcaaaa aagccaatag ttaagaaaat ttatgcccag atggccttgg ggtttagagg
     1081 aacacccaga caatggaaac tacaaatcat tgctggaatt ttactatctg cacttgtatt
     1141 gccagtgttt gtgtcagtgc actcaatagt ctcttgggac tttgctgttt tgataggtgt
     1201 tgaaggatgg cactcaacca tatttgcccc gtatttcata attggggcta ttcattcggg
     1261 agtttcagct gtcgccatgt taatggcact ttctgtctgg ctttacggat tggacaagta
     1321 tattaaacct gaccattttg acgctattgc tagattgcta attatagtgg ccactacatg
     1381 gtttttcttc ttctttttgg aatgggttta tgccttatat acattggaga gtcctgagat
     1441 cgctttgagg gagttacagg catttcaatg gccgtacgga cccttgtttg cgatttttat
     1501 catcacatcg tttgtgatac cagttccgat gtggttgttt aagagggtga gaagaagcgc
     1561 cgtattgatg ttttggacaa caatactagt caatatagga atgtggctgg aaagattctt
     1621 aattgtgatt ccaggccttg ttagaaggac gcatatgaca tttgattggg gtacctacca
     1681 cccgtcaatt gttgaaatat tggtagtagt ggaaactttc gctttcgtct gcttgggtat
     1741 gttgttgttt gctaaagttt tacccttgat tccattattc gacattaagg aagggatggt
     1801 aaaccgccgt ctcataaagg tgggtaggcg agttattccg gcgacgattc gtgaaggtct
     1861 tccccatcac tattatgaaa aaaaacatta aaggtagttt gagagagtat ttgttatggc
     1921 aaaaagaagt gtattaggtc tgtataccga agaagattct gctgctgatg ccctggataa
     1981 cctaagggag gcaggatttg aggaaggaga gtatgaaata ctcactggag ttccttaccc
     2041 tgagggcacc tttggggagg cagaaccaga gcataaactc tatagatggc cgttgattgg
     2101 agcagcatgt ggatttacca tcgccctgtt aatcacggct ggtactgagt tggcgtatcc
     2161 gttgataacg ggtggcaagc caatactatc gattccagca atggctatca tcatgtatga
     2221 agggtcgatg ctcggagcta ttatatttac catcataggg ataatttttg aatcccgtct
     2281 tccacgaata ttcatgggag cgtacagcga aaaaataaca gagggacata ttggtgtcac
     2341 tgtgacaaca gatgaaactc gcaccgacgt tgctgaaaca gctttgaata aggcaggtac
     2401 tgatggggaa ataattagag gctggaagta attaaaaaga agtcaaatat gtctaacaat
     2461 ggtttaaaca taaaaatcac tgcaccaata gttatggttg caattctttt ggtcaccttg
     2521 cttgtcaatt ccggttgcta caacagtaaa acgggagagg ttaaagtagg gggctctatt
     2581 agttttgagc tgccagtatt tcctgaaaca ggctcacatg cagttcaaat attttctgaa
     2641 atgcagtatt ctccttccta tcggtctcag gaagttccta ggctccttcc accggagggc
     2701 tctgttcctg tgacaggggc ggaggttaag attcttgagg ttgaagctct taaggcattg
     2761 tcgatgccgg atcatgatgg aagtcaggct gcaaaaatct attcggtaaa ttgccaagtt
     2821 tgtcatggtc aatcattgcg gggggatggc ccaatcacta ccttaagtag tgcacgaggt
     2881 gatggaagta aagcgtatag tggggcagct cctgtaaatc ttatttcaga aagagttaat
     2941 gatttgagtg atggagagat tttcggttat atcacatggg gaggaagacc agggttagct
     3001 gccgcagcca gaggcaaaga ttccacttca attatgcctc aattcggcta tttgctaaat
     3061 gaagaacaga ggtggcaatt agttcaatat attagagatc aaaataaata gattgacaat
     3121 cactgcttgt tgaaggtcaa acctatttac tcaacctgtt tcctcgctta gcttcaccga
     3181 cgactgaggc tacggcaaaa tcattggaat gggacaatga aagggatatc tcagacaaac
     3241 ccataataga agctctagct tgacctctgc cgtgcagttg aatataggga ggtccgcccc
     3301 tctctctttt gatttctata tctttccacc ctactcccct tatgcctgtt cccagagcct
     3361 tcataacagc ttccttcgct gcaaatcttg atgccaactg ggctgccctc cctttgcaat
     3421 acctttgctc tgaatctgta tatattcgat tcaaaaacct agcaccatat ttattcagca
     3481 cctcacctac gcgagctatt tcaatgatgt caattcctgt aattagcata tcgactattt
     3541 cctgaacatt taaaattgat atgatcggta taggtataat agtgtattca ataatcttgt
     3601 gaccactgcg gttagatatt taatgaaata taaacttatt gatgggaaag ccatatcaga
     3661 atcgataaaa cttgaagtga aaatgtcagt tcgtcggtta tatgaggaat cttcaatgat
     3721 accgtcacta tcagttattt tggtgggaga tgatccggcc tccagcgtat atgtgagaaa
     3781 caaaaagagc tcggcaatca acttggggat ggaaagccaa attatcaatt ttcctgaggt
     3841 tgttactaaa gaagagttgt tacggcaaat tcgaattctg aatcaagatg ataattgcga
     3901 tgggattctc atccaactgc cacttccggc ccatttggat gagaacgata ttttgtgcac
     3961 gattgatccc caaaaagatg ttgatggtct ccatcctcaa aatgttggct tattgtcaat
     4021 gggcgccggt tcattaattc cggcgacacc tgccggcatc agggaaatgc ttttaagatc
     4081 agagaatgat ccaaagggca aacatgtggt tatattgggt agaagtaata tcgtaggcaa
     4141 accattgtct aatttgcttt cccagaaagg gcacggaggc gatgctacgg ttactttgtg
     4201 tcatagtatg acgaataatt tgaaagatat tactaaaagt gctgacatat tggtggcggc
     4261 tattggaata ccaaattttg tgactagtga tctggtttct tctggtgttg tagccataga
     4321 tgtaggtgtt aatcgtattg aagatagcac tcgttcccgc ggctatcgat tgacaggcga
     4381 tatagatttt gaaggggtat cacggaaagc gtctgctatt accccagttc ctggaggggt
     4441 tggaccaatg acaatagcca tgcttttaaa aaatacttta aaagctgtct tacgaaagat
     4501 ctagaagcgt ttatgggttc cacaagatag ttagtggcta ggttataatt tacctgctcc
     4561 gtaaattgga tagtattgga cattaacgta tttttatagt acgaagtggg aattgctgag
     4621 aatataagta catttcatga ccacacataa aagagattat tacgaagttc ttggggtctc
     4681 tcgcaactca accgacgatg acttgaagaa ggcttttagg aaactggcgt tcaaatatca
     4741 tcccgatagg aataaggaac ctgaggcaga ggaaaaattt aaggaaatta atgaggccta
     4801 tcaaattcta tcggataagg aaaaaagggc tcaatatgac cgattcggtc atgctggggt
     4861 aggttcctct cccacagggg caaatggttc ttcggggttt ggagatgtat ctgggtttgg
     4921 ggacatattt gattcattct ttggaggttc tggatttggc agatcttcaa gggctcaaaa
     4981 ttcagctagg cgaggttcag acctacaata cacaatgacg gttgaattcg aagaggccat
     5041 tttcggagcg gaaagggaat tccaaatcca ccgcacagaa tcttgtagcc gctgtagggg
     5101 ttctaagagc gaacctggta gttctgtagc tacttgtggt acctgccaag gttcaggaca
     5161 agttcggaag gctcagcaga gtgtattcgg gcaatttgtg caggtcggag attgtaataa
     5221 ttgcaagggt tccggaaaga ttgtttccaa tccatgttca tcttgcaacg gtcaaggtcg
     5281 cgaaagaaaa aataggaaat tagccatcac tatcccggcc ggaatagagg aagataccca
     5341 ggttaggtta actggtgagg gagagcacgg gataaacggg ggacctcctg gtgatttgta
     5401 tgtagccttt aagatcaagg aacatgacta ttttgtgaga gatgggataa acattaaata
     5461 caggttgcct ataaacatag ttcgagcgtc cctcggaacc aaaattaaag tacctacatt
     5521 ggatggtgac tatgagatcg acattcctcc agggacacaa accggacgga tttttagagt
     5581 gagaggtaga ggggtggccc aacttagagg cgatagaagg ggagaccttt tggtgttaat
     5641 cgatgtaaga attcctaaaa agttagattc tcagcaacaa aaattgatga acgagctagg
     5701 agaatctctg cctgatactt ttgaggatga tgaagacaag gggatattcg ataaattcag
     5761 aagcgccttt accaattgaa gtggcttgaa ttaagtgttg aggctcctcc tgaatatgtt
     5821 gagccattaa ctcatatttt ttataggtat ggggaaggtg gtgtggcgat tgaaagcccg
     5881 gcagagttca acccggatga aggtgaaagg gccccgcagc cgcaaactct taaagtagta
     5941 acttttgtgc ccttagatga gtcagctaga ttacgaaaat ctaatataga aatcgctgtt
     6001 agattagtgg ctcattttgc atctatatct gaaataaaag aaagagaagt tgaggaggaa
     6061 gactgggaat ctaattggaa agagtatttt catccgatac gagtcgggtc taatctggtg
     6121 atatgtccta cttggcggaa acatcaatcc ttggatgacg acgttttaat aaaattggac
     6181 ccaggtatgg ccttcggaac tggtcatcac ccgacaacta ggatgtgtat ggaacttctt
     6241 gaaactctta ttgttggtgg ggagaaagtg attgatgttg gttgtggctc gggaatttta
     6301 tcgatatcag cagcaaaact aggcgctgga gatattcttg gcatagaagt agagtcgcgg
     6361 gcggtcgaag tggccttaga aaattgcgaa ttaaatggta tttctcctca aataaccata
     6421 ctgaatggca cttttcctga aatccgagtc gaaagtgcta gctttgatgt ggccctggca
     6481 aatattgccg ctaaagttgt gataaatttg tccgaataca tagtaggtgc agttgctaat
     6541 ggtggaagat tagtgctgtc tgggatttta aaaaactccc tgaaggacgt cgaaaaagaa
     6601 tactccttac atgaggtaca cttcgataaa gttcttgttg atggcgattg gacagctgtg
     6661 ttagctacca agaaaatagc gactgatatg tagcttgtaa ttgaattggg ttgttatgca
     6721 taggtttttt attgatccat ctgacataca ggatgggaga gtccaatttc ctgaagaaac
     6781 atctctgcaa atgaagaaag ttttgcggct taggagtgga aatgaagtgg tggtgtttga
     6841 tgggggtggt gtggactacc aggtccgctt ggaggtagtt gaggctaaag ccagttttgg
     6901 tactgtgcta tcgaccaatg actctaataa tgaaccagca attttcatca ccctttacca
     6961 agcgttgata agatctgaaa gatacgaata tgctttgcag aaaggagttg aggctggtat
     7021 ctctcggttt gttcctttta catctgagag gactcaggtt tcgactccaa gccattctaa
     7081 gatgggtcgt tggaggcgaa tcataaaaga ggcagctgag cagagtggca ggtctgtggt
     7141 gcctaaacta gcagacccta ttttattttc taatatgata aagaacttaa attctgttgc
     7201 gcttatacct tgggaacagg aaaaaaacca aagtattaag caggtgttat ccagatcgca
     7261 tcaagatcca ggattaaatg tgacatctat tgacctgatt ataggtccga taggtgggtt
     7321 tacaccacag gaagtttctg atgcagtaga ttgcggtgca acttcagttt ctttaggcaa
     7381 gcgaatatta ag
//
LOCUS       DASCNS010000245         4715 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001858561, whole genome shotgun sequence.
ACCESSION   DASCNS010000245 DASCNS010000000
VERSION     DASCNS010000245.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001858561"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="QF838_08820"
     CDS             <1..1045
                     /locus_tag="QF838_08820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60819.1"
                     /translation="GVMTGVGSVGMIIGTLYAGVALEYMSWRWIFFGRIPICILAILG
                     SVTVIRGVGNQNNNNNEATSFDWVGGVLAFVCLIAFVASMNIATAIGWLRVETFVCLG
                     VFIFSGTFFVRRQSIIPDPLVKLSIMKDLVVAGGFGANLFLYMGSFVNLFILPYFVGE
                     IIGASSFVLGTFLLLNAVSVSLFSPVGGYLSDKFGPGFITVLGLFIVLVALISYTALT
                     ADSSLEQIAFRMVFVGMGIGLFQSSNLSLIMGKMIPSDLGSGGAVSSMSRGLGSVTAV
                     TLLGWAFTSIYEGKSPEVDILQAGSSPDSIAAFMYAFQLSYIAGSVIVSVGLVSSLVA
                     WIGLKRSENSFVI"
     gene            1082..1837
                     /locus_tag="QF838_08825"
     CDS             1082..1837
                     /locus_tag="QF838_08825"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HJO60820.1"
                     /translation="MKIARFMLGLEETWGVILGDSIHAIHGSPYDIKNVGDRICSLSD
                     ITLLAPLTLTNKVPAIAANYGEKDDRDGPGIFMKQPGTYASHQGNIIYPRSCKSVIHE
                     AEVGVVISKTARHISESDAMDYVMGFTCVNDVSAKETVESDLGKGLSMRWKHFDTFCP
                     MGPHIETEIDDPGNLKIECFVNGELSTGGNTSEMIFAIPKLISWVSEVMTLQPGDIIP
                     TGCPETAEINIGDVVEVRIEGIGSLINNVIEDY"
     gene            1894..4281
                     /locus_tag="QF838_08830"
     CDS             1894..4281
                     /locus_tag="QF838_08830"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60821.1"
                     /translation="MGTFTKPSALAHISVLDLTGPEGNLCGKIFADLGADVTKIEPPD
                     GDKSRKIGPLASGVNQPDFSLYFANYNTNKYSVMLDLESLEGANKFIELSEKVDVVIE
                     NFRPGYMNQIGIGFERLSLNNPGIVMASISPFGQTGPYSSFIGDELIVQAMGGVMYCQ
                     GDEMKPPCVAPCEQISQLASFHAATGVLAAINHRNLTGKGQCIDVSMYEIAAQLLFNI
                     TRYTYHGDIARRMGSTPIIAPNGHYACSDGYISLAVLENRHWRELVRWMDNESLADPM
                     WEDMNFRRSQPEVVDIFVRDFIAGFTVEDFLEQAYSRHLAVSRVNEISDIAQSSQLKE
                     RNYFREIIDPQIGAYLTPGPPYRFGLTPTARDRPVPRLGEHQERVIGKARTEDWNSKE
                     ANDRLHDDHELPLRGIRILDLSRVWSGPFATRYLGDLGAEIIKVETNKHLDTGRIVTN
                     SSPQFLEINRSKKSITLDFAGPEGVELIKELVQISDVVVENFASGVLNRRGLGYETLR
                     KVKPDLVMISMPGYGTEGPKAEHVGYGQSLMSYAGLSNLWGFPDSPIENHSNVHFPDF
                     VSASTTTTAILAALEYRNQYRFGQHVEIAQVEAMAGSMGVALLDYFVNGKSWEPIGNR
                     NPWSAPNGCYPCEGEDEWCAISCSNEDNWNNLVNSMGNPAWAQDPKFQSRENRLINHD
                     ELDSYISQWTDNFKSHDLMNLLQQHLIPAGVVQTGEQLYNDPHLRDRGFIVSVDHSEW
                     GAVEHPGIAIKFSDTPGRITRGVPYLGEHNEEIFSNLLGVSENDMGQLLQKEVIS"
     gene            4321..>4715
                     /locus_tag="QF838_08835"
     CDS             4321..>4715
                     /locus_tag="QF838_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60822.1"
                     /translation="MMKASVKVLFKTPVRMPNGLQWFENELFVMDQLTDDIYVLGDNG
                     EVIRIINTETENGSGIAVGGGFIWTACNGTASARPPRQTDDHISKIRKIDLVSGETVD
                     SFNTPDGGGIHGLEWEDGNIWITAFNPKSL"
BASE COUNT         1293 a          875 c         1195 g         1352 t
ORIGIN      
        1 gggtgtaatg accggtgttg ggagtgtggg aatgataata ggcactttat atgcgggcgt
       61 cgccttggaa tatatgtcct ggcgttggat atttttcggc aggataccta tttgtattct
      121 agctatcttg ggttcagtca ctgttattcg aggggtcgga aatcagaaca ataacaataa
      181 tgaggcgact agctttgatt gggtcggcgg agtccttgcg tttgtatgtt tgatagcgtt
      241 tgttgcttct atgaacattg ctacggcaat aggctggcta cgtgtcgaaa catttgtgtg
      301 tttaggggta tttatttttt caggaacttt ttttgtcagg cgccagtcga ttattcctga
      361 tccgttggtt aagttatcaa ttatgaagga tttggtagtt gccggtggat ttggtgctaa
      421 tttatttctc tacatgggtt ccttcgtcaa cttatttata cttccgtatt ttgtgggaga
      481 aataattgga gcttcatctt tcgttttagg aacatttctt ttactgaatg ctgtttcagt
      541 atcactattt tctcctgttg ggggttattt atctgataag ttcggacctg gatttatcac
      601 agtgttgggt ttgtttatag tgttggtggc gcttatttca tatactgctt taactgccga
      661 ttctagcctt gagcagatag ccttcagaat ggtatttgtt gggatgggta taggcctttt
      721 ccagagctct aatttgagcc taatcatggg gaaaatgata ccttctgatt tgggttcagg
      781 tggtgctgtg tcatcaatgt ccagaggcct aggtagcgtg acagccgtta cacttttggg
      841 atgggctttc acttcgattt atgaagggaa atccccggaa gtagatattc tacaagcagg
      901 ctcatctcct gattcaatag ctgcttttat gtatgccttc cagctttcat atatagctgg
      961 atctgtcatt gtgtcggttg gactggtttc atcactagtt gcttggattg gtttgaaaag
     1021 atcggagaac tcgttcgtga tttagtctat aatcgtcaaa aattcaaagg tttggacgaa
     1081 tatgaaaatt gcgaggttca tgcttggatt ggaggaaacg tggggggtta ttttgggaga
     1141 ttccatacac gccattcacg gaagcccgta cgacataaaa aacgtcggtg acaggatttg
     1201 ttcactatca gatatcacac tactcgctcc tcttacttta accaataaag tccctgctat
     1261 agctgctaat tatggtgaaa aagacgatcg agatggtcca ggtattttta tgaaacagcc
     1321 tgggacatac gcttctcacc aaggaaacat catatatcca cggagctgca aaagtgtcat
     1381 acatgaagct gaggttgggg tagtgatatc taaaactgct cgccacatca gtgaatccga
     1441 cgccatggac tacgtgatgg gattcacatg tgtgaacgat gttagtgcaa aagaaaccgt
     1501 agagagtgat ctaggcaaag gcctttcgat gcggtggaaa cattttgata ctttctgtcc
     1561 tatggggcct catattgaga cggaaatcga tgatccgggg aatctaaaga ttgaatgttt
     1621 tgtaaatgga gagttgtcga ctggtgggaa tacatccgaa atgatatttg caatacccaa
     1681 gttgatcagt tgggtttcag aagtgatgac tttacaacca ggggatatta ttccgacagg
     1741 ttgccctgaa actgccgaaa tcaatatagg tgacgtggtg gaggtccgca ttgaggggat
     1801 cggttccctt atcaataatg taattgaaga ttattaagat aggcatctat tggcaatttt
     1861 gctataagtg gcgtttagaa aggaggttta ttgatgggta ctttcactaa accctccgcg
     1921 ctcgctcata tttctgtact tgacctcaca ggcccagaag ggaatttgtg cgggaagatt
     1981 tttgcagacc ttggtgcaga tgtcacaaaa atagaacctc ccgacggaga taaaagcaga
     2041 aaaataggac cattggcatc aggagtcaac cagcctgatt tcagcttata ttttgcaaat
     2101 tacaacacga acaaatatag cgttatgttg gatcttgagt cattagaagg agccaacaaa
     2161 tttattgaat tgtcagagaa ggtcgacgta gtcattgaga attttagacc gggctatatg
     2221 aatcaaattg gtattgggtt cgagagattg tctttaaaca atcctggaat tgtgatggca
     2281 tcgatcagcc cctttggcca aactggcccc tatagtagct ttataggtga tgagttgatt
     2341 gttcaggcga tgggaggcgt tatgtattgc cagggagatg aaatgaagcc tccttgtgta
     2401 gcgccgtgtg aacagatttc tcaattggca tcgtttcacg ctgcaacagg ggtattagca
     2461 gcaataaacc atcgtaatct cacagggaaa ggacaatgta ttgatgtttc gatgtacgaa
     2521 atcgcggctc aacttttatt taatataacc aggtacacat atcacggtga catcgcccgc
     2581 aggatggggt ccacgcccat aattgctccg aacggacact atgcatgctc agacggatat
     2641 atatcgttag ccgttctaga aaacagacat tggcgagaac tggtgcgatg gatggataac
     2701 gagagcctag ctgatccaat gtgggaggat atgaattttc gccgatccca acccgaggtt
     2761 gttgatattt ttgtccgtga ttttatagct ggatttacag tggaagattt tttggaacaa
     2821 gcatattccc gtcatttggc ggtaagccgg gtaaacgaaa tttcagacat agctcagagt
     2881 tcacaattaa aggaaaggaa ttatttccgt gaaattatag atccgcaaat cggtgcgtat
     2941 ttaacccccg gtcctccata tcggtttgga ttaacaccaa ctgcccgaga cagaccagta
     3001 cctcggttag gggaacatca agaacgtgtg attgggaagg cgagaaccga ggactggaat
     3061 tccaaggaag ccaatgatcg tctacatgat gatcatgagc tccctttgcg agggatcagg
     3121 atattggatt tgagccgggt ttggtcggga ccttttgcga ctcgctattt gggtgatctt
     3181 ggagcggaaa ttatcaaggt tgaaaccaat aaacatttgg atactggacg aatagtcact
     3241 aactcgtcac cgcaatttct agaaattaac aggagtaaga aaagcataac tttggacttc
     3301 gctgggcctg aaggtgtgga acttataaaa gagttggttc agatttctga cgtggtggtc
     3361 gaaaatttcg cctctggcgt actgaatcgc agagggctcg gttatgaaac tctaagaaag
     3421 gtaaaaccag atcttgtgat gattagcatg ccaggttatg gtaccgaagg acccaaagct
     3481 gaacatgttg gttatgggca aagcttaatg tcttacgcag ggttgtcaaa cctatggggt
     3541 tttcctgact cccctattga aaaccattcg aatgtgcatt ttcctgattt tgtgtcggca
     3601 agcaccacta ccacagctat tctcgccgct ttggaataca gaaaccaata tcgatttggc
     3661 caacatgtgg aaatcgccca ggttgaggcg atggcaggct caatgggggt ggcccttctg
     3721 gattattttg taaatggaaa atcgtgggaa ccgatcggga ataggaaccc ttggagcgcc
     3781 cccaacggat gctatccctg tgaaggagag gatgaatggt gcgcaatttc ttgctctaat
     3841 gaggacaact ggaataattt ggttaattca atggggaatc cggcttgggc tcaagaccca
     3901 aaattccagt cacgagaaaa ccgccttata aatcatgacg agttggacag ttatataagc
     3961 caatggacgg acaattttaa atcacatgat ttgatgaatc tgcttcaaca acatctaatt
     4021 cctgcagggg tagttcagac cggagagcaa ctatataacg atccacatct cagggaccga
     4081 ggcttcattg tgagcgtaga ccattcagag tggggggccg tggaacaccc tggaatagcg
     4141 atcaaattct ctgatactcc tggccgtatc actaggggcg tgccatattt aggagagcat
     4201 aacgaagaaa ttttttccaa tctgctagga gtgtctgaga atgatatggg ccagcttctt
     4261 caaaaagaag tgatatcgtg atttccaatg attgcaagac aagtgtaaga gtggaagtag
     4321 atgatgaagg ctagtgttaa agtgctattt aaaacgccgg ttcggatgcc caatgggttg
     4381 caatggtttg aaaatgagtt gtttgtcatg gatcaactga ccgatgatat ctacgttctc
     4441 ggtgataacg gagaggtaat tcgaattatt aacactgaaa cagagaacgg atccggaatt
     4501 gctgtcggtg gaggattcat ctggaccgcc tgcaatggta cagcgagtgc acgaccgccg
     4561 aggcagaccg atgaccacat atcaaagata agaaaaatag acctagtaag tggtgaaact
     4621 gtggactcgt ttaacacgcc agatggtggt ggaatccatg gacttgaatg ggaggatggc
     4681 aatatttgga taacagcctt caatccaaag tctct
//
LOCUS       DASCNS010000246         5923 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001858604, whole genome shotgun sequence.
ACCESSION   DASCNS010000246 DASCNS010000000
VERSION     DASCNS010000246.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001858604"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /gene="glyA"
                     /locus_tag="QF838_08840"
     CDS             <1..315
                     /gene="glyA"
                     /locus_tag="QF838_08840"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.3"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HJO60823.1"
                     /translation="HRFDLISNQNYLVMIFNVVKNGNHQFYQFRNAIPFDTKPPRQAS
                     GIRLGTPAITSRGMKVREATTVGRLILKVLTNIGDPSVEENVKHEILKLTSSFPVPGI
                     TS"
     gene            complement(352..1719)
                     /locus_tag="QF838_08845"
     CDS             complement(352..1719)
                     /locus_tag="QF838_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60824.1"
                     /translation="MIDLLSIARTEADGGDLLNLLSGLFEPSEVRPEGYSDAVVWQGG
                     NHDGVVNPVAHSLTDAYALLGTIAAVDILGLPFYAVPMWSQYRHDTKLASLGWFGNMP
                     CTSIKWSTAGDAYVNDGHGNKVVVMGKSANAPLRTQAGVYCNVRPYGPITVVRCMPCL
                     PYSQDRDKFVVDDAGIVHSEMNTPGIQMAYANRLFLKMVNDSGHLGRVAMKPTQAMED
                     GINAGLHSWLLKGTKFEVGRRYAVDPYEEHKERIDRIVSLLPPDFKDGMEHWGGRIEQ
                     HISDTNYGLLIWDLIEKRDTIVLATDLDGDRLTDLLADISDPLRAFGDKVVQYVGKNV
                     NMWDAWSEMGYTTGNGRDMTSVMHRMDGPPIHEQTLGSADAMLLRLLDGDESLGGTKR
                     PYDPRIHFVLIRDAYIDANPGNSELRNWLDASLAAFDRIYGENRPGFLDGYKALKEAI
                     TPWGS"
     gene            complement(1891..3534)
                     /locus_tag="QF838_08850"
     CDS             complement(1891..3534)
                     /locus_tag="QF838_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60825.1"
                     /translation="MVKVSGNYLIARTLKEEGVDTLFYLMGGPNFDTIMECQDVGIRT
                     VDFRHEQAAAMAAHAYSRVTGKPSVVMAASGPGTLNLITGVYTASIDCAPMIILGGAS
                     AVHEIGRDTFQEVDQVGIMQPLCKYWHRPTMASRFPEVVSTAYRQAQSGRPGPVYIDC
                     GGDVLYEEVEEDSIPTPSRAVERTRPVATSGDIERAMGLIANAERPLLFAGGGAFFSG
                     AAPELEKFVDVTSIPFYTAPMSRGLVPEDHAVSFQGARSTAMRESDVVLVVGTRMNWM
                     MNYGQRFAPGAKIIQIDINEAELGHNRDIEMGIFGDAKAVMSQLLELAEQRSPDFSGA
                     LESPWIVGLREANEKRMAQTAPLLNSDQTPIHPLRLCKEIDEFLDRDAIISVDGNEIL
                     HFGRQSISTYMPGHRLNSGVTGTMGVGLPYGIGAQVAKPDKQVLVLHGDGSMGMNAME
                     IDTAVRHNLPVVTVISNNEGWTARTPGKRKPGRELGFTEFDRMAEALHGYGEKVEDPT
                     EIKPALDRAFSSGKPAVVNVRVEPTSAGVSRSWGGSRME"
     gene            complement(3624..4298)
                     /gene="rnc"
                     /locus_tag="QF838_08855"
     CDS             complement(3624..4298)
                     /gene="rnc"
                     /locus_tag="QF838_08855"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HJO60826.1"
                     /translation="MLAKVEHKFIDKELFHMSLTHSSWVNESDGREDHSNERLEFLGD
                     AVIDAVIAQSLYEKFPDFNEGDLTKLRSNIVSGVSLAKVARKLGMGNYLLLGKGEEAS
                     GGRDKDSNLANAFEALVGALLLDRGYAAASQFVLQNLNIESPGTGFVKGRNDPKTDLQ
                     ILVQSKTSFTPDYKLISRDGPDHNPIFVVDVLIGDKIMGNGVGSSISKAEMQAAANAL
                     TKFQNH"
     gene            complement(4321..4950)
                     /locus_tag="QF838_08860"
     CDS             complement(4321..4950)
                     /locus_tag="QF838_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_264297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4909..4911),aa:Sec)
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HJO60827.1"
                     /translation="MNKMEFDIFYDFAUPYVYNAAVWLDKVKEHYGDDLKLNWRNFSL
                     QQINAKDPGDWRVWQEEDYTSTRSLMASIAGEAAKRQGDERFDKFFLALLTERHGGSR
                     APLNDDSMFIKLAEGCGLNIEQFKSDIKDPKLVEIIANDHTEAVDIHGAFGTPTFVFG
                     NGQSAYLKTFIPPEEESVDAFKHFLGLFESRSYIGEVKRPQPPWPKGAV"
     gene            5200..>5923
                     /locus_tag="QF838_08865"
     CDS             5200..>5923
                     /locus_tag="QF838_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HJO60828.1"
                     /translation="MANESAELLKLMKDNGCDILDLKFTDLPGSWQHFSVPISEIDDS
                     LVKNGVGFDGSSIRGFQSIDESDMLIVPDAPTAKVDPFFKGTVTCIADIKDPVSGENY
                     TRDPRNVAKKAEAYLKSTGIGDDSFWGPEVEFFIFDSVRFGYGSNHGFHEVNSDEGIW
                     NSGSEYMLDGETLNKGNRPRHKGGYFPVSPVDTAQDLRSEMVRSLVNDFGLRIEAQHH
                     EVATGGQAEIDMRFGPLLQTADN"
BASE COUNT         1672 a         1447 c         1155 g         1649 t
ORIGIN      
        1 catcgctttg atttgatttc taaccaaaac tatttggtta tgattttcaa tgttgtcaag
       61 aatggaaatc atcaattcta ccaattcaga aatgctatcc cgtttgatac aaaaccaccc
      121 aggcaagcta gcggcatcag acttggtaca ccagctatca caagtagagg aatgaaagta
      181 cgcgaggcca ctaccgtggg taggctaatc cttaaggtcc tgaccaatat tggggaccca
      241 tcggtggaag aaaatgtcaa gcatgagatt cttaaattaa cttcctcttt tccagtcccg
      301 ggcatcacga gctagcctag gaaggttagc ctgtgatgca attggaataa cttaagagcc
      361 ccaaggagtg attgcttcct taagtgcttt atacccatcc aagaagccag gtctgttttc
      421 tccatatatc cgatcaaaag ctgctaatga ggcgtctaac cagtttctca actcagaatt
      481 cccaggattg gcatctatgt aggcatctcg tataagcaca aaatgaatac gaggatcgta
      541 tggtctcttc gttccaccca aagattcatc cccgtctaaa agcctaagta acattgcatc
      601 agcagagcca agggtttgtt catgtatcgg cggaccatcc atcctatgca tgactgaagt
      661 catatccctt ccgttaccgg tggtatatcc catctcagac caagcatccc acatattcac
      721 atttttaccg acgtactgta ctaccttatc cccaaacgct cgtaaagggt ctgaaatgtc
      781 tgcgagtaga tcagtcaatc tatccccatc caagtcggta gccaacacta tagtgtctcg
      841 tttttcaatt agatcccaga ttaataaccc ataattggtg tctgagatat gttgttcgat
      901 cctacctccc caatgttcca tcccgtcctt aaaatctgga ggtagcaatg agactatccg
      961 atcaattctt tctttatgct cttcgtatgg gtcaaccgca tacctcctcc ccacttcaaa
     1021 tttagtgcct tttagtaacc aagaatgcaa tccagcattg ataccatctt ccatagcctg
     1081 ggtaggtttc attgcgaccc gacctagatg gccgctgtcg tttaccatct tgaggaaaag
     1141 tcgattagcg taggccattt gaatacctgg tgtattcatt tccgaatgta caattcctgc
     1201 atcatccact acaaatttat ccctatcctg tgaatatggt aggcaaggca tacacctaac
     1261 aacagtaatc ggaccatacg gcctaacatt gcagtagact cccgcctgtg ttctgagggg
     1321 ggcgttggct gactttccca tcacgacaac tttattgcca tgcccatcat tgacataagc
     1381 atctcctgct gtggaccatt taattgaagt acatggcatg tttccaaacc aacccagaga
     1441 agctaattta gtatcatgcc tatattgtga ccacattggg acggcataaa acgggaggcc
     1501 caatatgtct actgcagcga tggtccccag aagagcatag gcatctgtca aagagtgggc
     1561 aacgggattc acgacaccat catgattacc accctgccag acgactgcat ctgaataacc
     1621 ctctggcctt acttcagatg gttcgaataa tccagacaaa aggttaagta aatctcctcc
     1681 atccgcctct gttcgggcaa tggataataa gtcaatcatg aaaacacctc ttttattttt
     1741 ttgtcagctt tattggatat taccaaatta cggcaacaga ttgtccgaat agagaatata
     1801 tttgaattga gttctatagt tcatatattc aaaagggaga gtatcttgag tttagaagtg
     1861 attcaaataa atacaagacg taccgacatg ttactccatt ctggaaccgc cccatgatct
     1921 agatacgccg gcggaagtgg gttcaactct cacattaact acagctggct tcccactcga
     1981 aaaagctcta tctaaagcag gtttgatctc agtaggatcc tccacttttt ccccatagcc
     2041 gtgcagagct tcggccattc ggtcaaactc agtgaaccct aattctcgac caggttttct
     2101 tttaccaggg gtgcgagcag tccaaccttc attattactt atcacagtca ctactggcag
     2161 gttatgacgt actgcagtat ctatttccat ggcgttcata cccatagacc cgtcaccatg
     2221 cagtacaaga acttgcttgt ctggttttgc tacttgcgct ccaatcccat atggtaatcc
     2281 cacccccatt gtcccagtca caccggaatt taaacgatgt ccaggcatat atgtcgatat
     2341 agattgcctt ccgaaatgca aaatctcgtt tccgtctacc gagatgatgg catccctgtc
     2401 taaaaattca tctatttctt tacataagcg taatgggtgt ataggtgttt ggtcggaatt
     2461 gagcaatggg gcagtttggg ccatcctttt ttcattggct tcacgtaacc ctactatcca
     2521 aggagattcc agtgcaccag aaaaatctgg gctcctttgt tcggctaatt ccaataattg
     2581 gctcatgact gcctttgcat ctccaaaaat gcccatttct atatcccgat tatgaccaag
     2641 ctcagcttca ttaatatcta tctgtatgat ttttgcaccc ggtgcaaaac gttgcccata
     2701 attcatcatc caattcattc gagtcccaac taccaacaca acatctgatt ccctcatagc
     2761 agtggagcgt gctccttgaa acgaaacagc atggtcttcc ggaactaaac ctcttgacat
     2821 gggagccgtg taaaagggga tgctagtcac atcaacaaac ttttctaatt ctggagccgc
     2881 gccagaaaaa aaagcgcccc cgccagcaaa taacaacggc ctttctgcgt tagcgataag
     2941 tcccatagct ctttctatat ctccagaagt agccactggc ctagtgcgtt ccactgccct
     3001 tgaaggtgtt ggtatcgaat cctcttccac ttcctcgtaa agtacatctc ctccgcaatc
     3061 gatataaact ggccctggtc gaccgctctg agcctgtcgg tatgcagtac taacgacttc
     3121 cggaaatcta gaggccattg tgggtctgtg ccaatatttg cacaagggct gcattatgcc
     3181 tacctgatcc acttcttgaa aggtatccct tcctatctca tgcactgcac ttgccccacc
     3241 taaaataatc ataggggcac agtcgataga tgctgtatag acccccgtta ttaaattcaa
     3301 agtaccaggt ccggaagcag ccatgaccac actaggcttt cctgtgacgc gagagtatgc
     3361 atgggcagcc atagcagcgg cttgctcgtg ccggaagtct acggttctaa ttcctacatc
     3421 ctggcactcc ataatcgtat cgaagttggg tccacccatt aggtaaaaga gcgtatcgac
     3481 tccttcttcc ttcaaggtcc tagcaataag gtaattaccg cttactttca ccatgcgaaa
     3541 tatctcctga aaatcggatt aagcttagtt aacgaaggca aggttatcac agacccaaaa
     3601 aaagccacaa aaccaataaa tcttcagtga ttttgaaatt tcgttaaggc gtttgcggca
     3661 gcttgcattt cagctttact aatactagac ccaaccccat tacccataat tttatcccca
     3721 atcaacacat caacaacaaa tataggatta tgatcaggtc catctctgga tatcaactta
     3781 taatcaggtg taaatgatgt ttttgattga actaggattt gaaggtctgt tttagggtcg
     3841 ttacgacctt tcacaaaccc agtcccgggt gattcaatat tcagattttg caaaacgaat
     3901 tgactggccg ctgcatatcc cctgtctagg agcagtgccc caaccaaggc ttcgaaggca
     3961 ttagccaaat tcgaatcctt atctcttcct ccggaggctt cttccccttt gcccaataac
     4021 aaatagttac ccatacctaa cttccttgca acttttgcca acgacacacc actcacgatg
     4081 ttcgatcgca gtttggttaa gtctccttca ttaaaatccg gaaatttttc atacaacgac
     4141 tgcgctatta cggcgtcaat gactgcatcc cccaaaaatt caagcctctc attagagtga
     4201 tcttcgcgac catcggattc attaacccag gaagagtggg taaggctcat atggaataat
     4261 tctttgtcta tgaacttatg ttcaaccttc gccaacattt agaaatctat ccagatatga
     4321 ctagaccgcc cctttgggcc aggggggctg tggtcgtttc acttctccta tgtatgacct
     4381 actttcaaac aatcctagaa aatgtttaaa cgcatccaca gactcctctt ctggaggaat
     4441 aaaggttttt agataagcag attggccgtt accaaaaaca aaggtaggag tcccgaaagc
     4501 tccgtggata tcaaccgcct cggtatgatc gtttgcaatt atttctacaa gttttggatc
     4561 tttaatatca gatttaaatt gctcaatatt caacccacag ccttcggcca acttgatgaa
     4621 cattgaatcg tcgtttaatg gcgctcttga cccaccatgt cgctcagtaa gcaaagccag
     4681 gaaaaattta tcgaaacgct catctccttg ccttttggcc gcctcccccg ctattgaggc
     4741 catcaaagat cgcgtggaag tataatcttc ttcttgccaa accctccaat cgccaggatc
     4801 tttcgcgtta atctgttgta aggaaaaatt cctccagttc aactttaaat catccccata
     4861 gtgctctttg actttatcca gccacacggc tgcattgtaa acgtagggtc aagcaaaatc
     4921 gtagaatata tcaaattcca ttttgttcat atttatctcc aatctttgta aggctactgt
     4981 cgttagttta ccttaaagcg actgaaatga tacaactcta ccgcaactct taaagcgtct
     5041 gagtccgcta ccctagtccg ctaccctagt tggttgttac attttgttaa cattgacgca
     5101 ataacaaagt aacagcacgg taagataatt ataaaaagac aaagctgggc tcattgttct
     5161 accgaatgac cacttagaaa aaattggaga attgctttta tggcaaacga atcagcagaa
     5221 ttacttaagt taatgaaaga caatgggtgc gacatattgg acctaaagtt cactgattta
     5281 cctgggtcgt ggcaacattt ctctgttcca ataagtgaaa tagatgactc attagtgaag
     5341 aatggtgttg ggtttgacgg atcaagtatc agaggatttc aatcaattga cgaatccgac
     5401 atgctgatcg tcccagatgc ccctacagca aaggtagacc cgttctttaa gggcacggtg
     5461 acttgcattg cagacatcaa agaccctgtt tcaggtgaaa attacactag agaccctaga
     5521 aacgtagcca agaaagccga agcatatcta aaaagcacgg gtattggcga tgattctttc
     5581 tggggaccag aagtagaatt ttttatattt gactcagtaa gatttggata tggatcaaat
     5641 catggattcc acgaggttaa ttctgatgaa ggaatatgga actctggaag cgagtacatg
     5701 ctggacggag aaacactcaa taaaggcaac aggccgagac ataaaggtgg ctattttcca
     5761 gtttctccgg tagatactgc ccaagatctc agaagtgaaa tggtaagaag ccttgttaat
     5821 gactttggcc tgagaataga ggctcaacat catgaagtag cgactggagg ccaagcggag
     5881 atagacatgc ggtttggccc tctgcttcaa actgccgaca atg
//
LOCUS       DASCNS010000247        22459 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001867745, whole genome shotgun sequence.
ACCESSION   DASCNS010000247 DASCNS010000000
VERSION     DASCNS010000247.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 22459)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22459)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22459
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001867745"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="QF838_08870"
     CDS             complement(<1..906)
                     /locus_tag="QF838_08870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HJO60829.1"
                     /translation="MEFGFYLPTHGPLAKRDPILEIASHAENIGFDSMVAGDHVIAPI
                     NPESQYPYSVGSEVPWDSSGEHLEMITELAFLAGITSKAKLVTSVMIVPHRNPVLTAK
                     MLSTIDVLSGGRLVVGVGVGWLEEEFESLDTPPFNRRGKVTDEYISIFKELWSEEEPS
                     YDGEFYSFKRLQFSPKPIQKPGPPIWVGGQSRAAIRRVVKMADAWHPVGANPASPLEP
                     SEMAVEIDYMDSYCEKIGRNRSEISIVLKAPVYDKENSFGGNRRRFSGNPEQIVADIK
                     EYENLGVSHIVLDTRSADLNKSLEKM"
     gene            complement(929..1711)
                     /locus_tag="QF838_08875"
     CDS             complement(929..1711)
                     /locus_tag="QF838_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60830.1"
                     /translation="MAFADVNGVSMYYEVHGEGPPVVFAHGSGGNHISWWQQIPYFSK
                     HYKCVIFDHRTFGRTPDESEPKGRTAFADDLEGLLNHLGIEKTAIVAHSMGGRTASVF
                     AIRNPERVSALVLSGTNGGSDNEESREVRRKHKASPPFLPEGAIKALSPEFSEQFPEK
                     SLLYRQIMRMNPRHSPDFLAPRPNHSGSTDGRFRDLGIPMLFMVGREDKLVHPKTIEI
                     ASSLIPHSEFIMVDRAGHSVYYERPEFFNDLVHKFLKINLTL"
     gene            complement(1743..2501)
                     /locus_tag="QF838_08880"
     CDS             complement(1743..2501)
                     /locus_tag="QF838_08880"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO60831.1"
                     /translation="MNRLEGKVALVSGAAKGQGAEEVALFAAEGAQVLFGDILDDLGL
                     EVEKNIAAAGHNAKYIHLDVTNSQDWERAINLAESEYGKLDILVNNAGIALPQDYLDV
                     SSEDWDRTMEINSKGVFLGIKYAVPAMQRAGGGSIVNISSIAGIVGRPLSSPAYAASK
                     GAVRVLTKSTAGRFASENIRCNSVHPGPINTDMIKSVTNSVDPESRVGSIPLGRIGQS
                     IDVAYGVLYLASDESSFVTGIELVIDGGVTGVRE"
     gene            complement(2523..3362)
                     /locus_tag="QF838_08885"
     CDS             complement(2523..3362)
                     /locus_tag="QF838_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJO60832.1"
                     /translation="MQEKLQIGNLEILSLMDMVPPARSPEDFFPDVDIALWDPYRSEI
                     LDKGMVQLYYGCFVVRSKGKIVLVDTGIGPGPHATRGGQKGDLLNDLGRVGIKPEEID
                     IVVHSHLHIDHVGWNLDLSSDPPRPYFPNARYLVPSIDWDYFTRPENLINAPWVSENV
                     VPLKALSLMDLFGDGFNVTDEITSLITPGHTPGHMSFLLNSGGQKAAIIGDAIHSKVQ
                     ISEPSWCAGVDVDKIASERSRRDLIERSVSQGMIIAAGHFHPNEHFGRVHQHGDVTTW
                     ETV"
     gene            3486..4403
                     /locus_tag="QF838_08890"
     CDS             3486..4403
                     /locus_tag="QF838_08890"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60833.1"
                     /translation="MEKIKVGFIGLGNMGGGMSMNVAKAGYDMVVHDINKAAATNLLE
                     LGAEWAGNPKELAESSDVIFTSLPGPVEVEAVSMGNEGLIKGVSNGSIWIDLSTSSPT
                     LIRELSEEFEKQGTSVLDAPVSGGVKGAETGKLAIMVGGSENIFNQIKPILDSFGDKV
                     SYTGSIGSGSICKLMHNTIGYGMQMIISECLTLGVKAGVDPENLFECIKNGSVGRGNL
                     LNVTLPDTYLQGKFDPPNFALKLARKDVSLALELGREYDVPMEAASHAYNELTAAMNR
                     GWENKDSRISLLLQEERAGNIEVRLDNSK"
     gene            complement(4421..5608)
                     /locus_tag="QF838_08895"
     CDS             complement(4421..5608)
                     /locus_tag="QF838_08895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60834.1"
                     /translation="MITNFDSLYAGHVDLANVGYAGTPVNDRTFGNEYLVSAFDKATE
                     IAQTMDRCGYDTFWMAEHHFQPEGYECLPNVLMMALHLAHLTKNIRLGCGFNIAPMWH
                     PLRLAEDYATADVLTGGRVVFGVGRGYHTREVETFGSPLLDQPANRDLFEEQVDIIFE
                     AFNKDSFSHKGSYYTIPPEVPYRGYELKEITLVPRPVNRPVECWQPIQSATPRGLDFM
                     AKHGMSGIIGGGSSEGGAMDKAVEGFRDAYSRIGVDTELGENLAIGFHFHIADTKEQA
                     MKEVGNFYEENLKMFGPLRLVKALSDDQITAMGNPETALKAGLPTIEDAVEAGGVLAG
                     PPDLIIEQLKSLEKKYPGLNRVSVSHPVGTLKSVIIEQLERFSEEIMPAFKGSVAEMV
                     PGD"
     gene            5785..9180
                     /locus_tag="QF838_08900"
     CDS             5785..9180
                     /locus_tag="QF838_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HJO60835.1"
                     /translation="MTLKKILIANRGEIAIRIARAASDMNITTVSIYSEDDASSLHVQ
                     STDESFGLDGEGVAAYLDIDQVVEIAKKSGADSIHPGYGFLAENPGFAKRCQEEGIVF
                     IGPSLEMLELFGDKGKARLAAQKSNVPTPAGLEGPITLESAKDFFSNLEEGSGMMLKA
                     IAGGGGRGTRAIKNPSEVERLFKRCQSEAKRSFGNGDLYAEQLVENARHIEVQIIGDN
                     FGNVTHLWERECSIQRRYQKLVEIAPAPFITQSLRDQIIESAINLAVDVGYCNLGTFE
                     FLAYQSGGSEYDSFVFIEGNARLQVEHTVTEEITGIDIVQSQIRLADGSTLSDIGLDP
                     DNPPSVRGYAIQNRINCETISNTGQIKPSTGKITKYGAPGGLGIRTDGFGYSGYETTM
                     AFDSLIAKLICHSNSDNFLDAVNRSKRALSEFDIEGVETNIEFHLNLLSHPEFISGNI
                     TTLFLDDNLAELIPDASSSTGAANTSQPLKADEGFAGANLKTKDPLALFDYDQQVKST
                     QQESEPELTTPQGPDGTVNVVAPIQGTVISIDVDLGQEVTKGQQLLVMEAMKMEHMVT
                     SNISGIIRGITINEGDTIRENYPLFHIEESDVSVSSKDLNQELDPDYIRSDLQELNER
                     RSHIFDENRPSAVDRRRSKGQRTARENIADLCDPDSFVEFGSLAVAAQRLRRDEDWLR
                     INTPADGLICGVGTVNGDMFTEKDSRCLAVSYDYTVFAGTQGNRNHYKQDRVYELAHR
                     FRLPVVLFGEGGGGRPGDTDKMGGIGMDTTTFTTWSRLSGLVPMVGVVSGRCFAGNTA
                     LVACCDVIIAAEDTTIAMGGPAMIEGGGLGVYTPEEIGPLSFQVPNGVVDILVKDEAE
                     GVEVAKKYISYFQGATEEWEENDQRVLRHSVPENRKRIYDMRSIIETIADKDSVLEIR
                     KDFGIGIFTALIRIEGKPMGLIANNPHHLAGAIDSDASDKAARFLQLCDAFDLPIVSL
                     MDCPGLMVGPEVEKTALVRHCGRLFNTGANLTVPMFGVIVRKAYGLGVQAMCGGGSLV
                     PFFTVAWPTAEFAGMNIEGAVKLGYRKELEAIEDPEERIKEYEGMVEKSYERAKAVNA
                     GSYFGIDDVIDPANTRKWLVAGLKSLPPTPPRTEKKRQYIDTW"
     gene            9188..10447
                     /locus_tag="QF838_08905"
     CDS             9188..10447
                     /locus_tag="QF838_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60836.1"
                     /translation="MPTESQNPVHAQVLEGVKIIDLTGPSGFYCTKLLADLGADVIRI
                     ETGSTAELETFAPFYQGNKDIDSSLYRWHFHTNKRSVELDLASQQGKDTFLHLLEVAD
                     VLITTEPQNLANATVIKKAEIRTRYPQLIHTVIRAFSSRSAYSEYKATDIVALAMSGL
                     MAITGFPEDPPNQMGGEQAYHMVSMHGAIGTLVALLDRDLTGKGKDIEVSMQECTSMA
                     TLQTANLNYYIWQNLNRQRTGLNHPFSVPAADETLPTYPKTLYKCLDGWVSYAAHLAP
                     PGAWERFLQWLEDHDSQQDLRDPKYLDPEIRQKNQNHINEVMAEHCSKLKAQELYHSA
                     QSYKLLCVPVQNAADLIADKQLEERNFFKTVKHDNCSEDFIYPGAPYKLSETPWDIKN
                     NAPKIGQHTKEVLDEWLNPANAKVSYE"
     gene            10440..11660
                     /locus_tag="QF838_08910"
     CDS             10440..11660
                     /locus_tag="QF838_08910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60837.1"
                     /translation="MNSPRLPLSNIRIADFGWMIAGPLATRVFANFGAEVIRIESGSR
                     IDEIRVVGARPEGIESPNVAGVFNDINTSKLSVTLDLNTDEGLSLAKNVIEISDVVTN
                     NFRGDRMGKWGLDYDNLKKIKPDIIALSMPMMGSDGPHQDYGGNGINIIAGAGISSIT
                     GYPDKAPVGTGSLYPDFSGNPNHASIALLAAIRYRNRTGKGQFIDLAQYESTVSLLGT
                     SILEYTANGKLPDRPGNRSQNMAPHNVYRCKGMDRWCAIAVSTEEEWQNLKKGLGELE
                     WMNQPKFATFNQRKANEEELDEGISNWTSDKDAYEVMNLLQSHGVPAGVVQDTEDLLK
                     RDKSFTDTHVIDMPHPEIDSMKVHGETISMSGVTPKVDRAPLLGEHTEYILGDLLGVA
                     ESKIDDLYVSGVLK"
     gene            11675..12463
                     /locus_tag="QF838_08915"
     CDS             11675..12463
                     /locus_tag="QF838_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60838.1"
                     /translation="MANKLDGDVAIVTGGNSGIGEATAHLFAKEGAKVAILARREKEG
                     KEVESAIKASGGEATFFQCDVAERGQVDEVVENVVSTYGKISVLFNNAGGGDKGNFPD
                     ETDEGWDRVIAVNLNGTFYVCRAAWSHLIESGNGRIVNMSSLAAQRGFSKNMYDLVGR
                     GPSSSYYAAKAGVDAFTRYAAGMGGQHNIRVNGVRPGQIITPLTDVGGGNHSLKGAFD
                     FIQILEGAGYPDDVANTVLFLVSNDSRFITGEIINVDGGMPGKL"
     gene            12555..13742
                     /locus_tag="QF838_08920"
     CDS             12555..13742
                     /locus_tag="QF838_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60839.1"
                     /translation="MQYTFTEKEEDFRSELLEFLDKELPEDWNSLSPESHFSKENLPF
                     TKSMAKKLAEKGWLTMAWPEEYGGQGRSIMEQLIYREEMTYQDVPGTDLGTGAISWVG
                     PTLMIAGTHEQRKEHLPPIGNGDAYWCTLYSEPGSGSDLASLQTQAQKDGDDYVINGS
                     KIWTSSAHIADFGWLAARTNPEAPKHRGISLFMVDMKSPGVTVRPIYNMAGSHEFNEV
                     FFEDVRIPSKNLVGEENRGWYTLAVALDFERSGVGYSANARRILESLTVYVKSAHKNG
                     NLLSENQLVRNRLAERHIETEVSRSMSYKVAWMQSNGMVPNAEASMSKLFGTELVQRV
                     ANTAMNIIDMKGLLSHESDLSPLEGAVQKMYLSSFSSTIAAGTSEIQRNIIADRGLGL
                     PRA"
     gene            13780..15039
                     /locus_tag="QF838_08925"
     CDS             13780..15039
                     /locus_tag="QF838_08925"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60840.1"
                     /translation="MESLCPPTQERLLPYTILDLAEGGHNICGRLLGDMGAEVIKIEP
                     PGGSQTRLRGPFTKNSDGKPRSLYWASYNSNKKGISLLLDTDEGRQIFLKLVNKSDAI
                     LESFQPGYLDSLNIGFETLAKINPQLVHTSMTPFGSTGPYAGFISTDLITSSMGGMPF
                     VSGDEDRPPVRISFPQAELVAGSQAFGATVAALRHSRNSKTGQHVDVSEQIAVMWTLM
                     NATPFPQLHGTDVTRAGAFRKRGPIDARHVFRCADGYVSMNAQAKTLDGFIRWMSEEI
                     EIEKEILGFNIDKWNLKPDSDPNGKEASEFKKVEKAIESFLSIKTKMEIFERALSAGL
                     LVAPCNTVEDIRKSPQLEARSFWIDVYHPEFGKDITHLGPYMKMSETPLKVYSPSPSV
                     GSHNEEIFKSLGIGKSEIEALRKRGII"
     gene            15039..16295
                     /locus_tag="QF838_08930"
     CDS             15039..16295
                     /locus_tag="QF838_08930"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60841.1"
                     /translation="MQNNTSELLFDGLKVLDFTWVGVGPITTKYFADHGADVIKIESV
                     SRPDVLRGAPPFKDAIPGINRSQFSGNYNSSKRNLGVNLSIPKSVDLIKDIIVQWQPD
                     VIAESFTPRVMKSWGLDYLSVKRIAPDVIYFSTCLMGQYGPHNNYAGFGQLAGAMAGF
                     YEITGWPDRGPAGPYGAYSDFLNPPVAFGSIVAALDYRDRYGKGQHIDLSQYEGAMHF
                     LAPHIMKYNEDGFILGRMGNEDEEMSPHGIFQCLDKERGLTDTEESWVAIAIESESQW
                     TEFSKVLDLPEAISNQNLAERKLNKDQIDNLISNWTLQKDARQIMNLLQSHEIPCGMV
                     QSQSDMWEDPQLKHTDFFQWLEHSEVGPMPYDGLQFTLSKTPGSLTRAQAMVGEHNLE
                     ILDEILSMGPSEAANLLNEGILEQSF"
     gene            16295..17488
                     /locus_tag="QF838_08935"
     CDS             16295..17488
                     /locus_tag="QF838_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HJO60842.1"
                     /translation="MTKGALDGVKIVDLSRMAPGPFCSMMLGDMGADVIKVEAPPTSR
                     IIASKIINDAETRKKAASNPLNRNKRSIVLDLKEKDGIKILHQLCQKADVFIEGFRPE
                     VVTRLGCNYETIKEINPSIIYCSISGYGQTGPYKDLVGHDVNYISVGGALGLIGSKNG
                     TPSIPYNLIADFAAGGMNAAYSIVCALFSRTNTGRGQKIDTSMSDGVTYLLSAIPGEY
                     FNSGLIPKPGQMTLNGGVPYYNVFECQDGKYISLGCIEPNFWKNLCSTLNLEQFASSQ
                     HNEHEYQLIMRELEATFKTKKRDEWWDIFREAEDVAAAPVYEFNEVLDDPHMKARNMF
                     IEAGSVDGAVITQVGFGSKLTDTPGAVRNLGTIPGAHSENILREIGYSADEIKKMVDS
                     EVVFT"
     gene            complement(17810..18217)
                     /locus_tag="QF838_08940"
     CDS             complement(17810..18217)
                     /locus_tag="QF838_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HJO60843.1"
                     /translation="MVFISEEADVFLNQTQIAVISTVGADYRPHSVPIWYGWDGHCAS
                     MFTGTNTLKWKNLSHNPYATLCVDFREPPYKSVVIHGKVEEIKIPLQDFVKKLAIRYY
                     GEEEGREFADLYPDDKKDVVIFKLIPDKIVEEL"
     gene            18314..19375
                     /locus_tag="QF838_08945"
     CDS             18314..19375
                     /locus_tag="QF838_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014893.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HJO60844.1"
                     /translation="MSKVGLAIVGCGTISQLNVPGYLEHPNCEIVALCDVNEDRAIAK
                     AKEWKINPRIYTDYDSVLADEAVDAVELLSPTHLHASQIISGLNAGKHVSCQKPIATS
                     MDEAKQIRKAVENNEKIFRVTENFLYYPPITKAKELIENGAIGTPSLVRIRTIRGLGM
                     ETMIRPEKDAYVWRRDPSKNPGGMLYDDGWHKYATAISWVGGVEKVTSMVTRTDDFLA
                     DTPSAAVMKVKDKDCLITIDYSSANEMPIKGKYYSADEFFEIIGPKGTIWVTRCTGEL
                     LDMPPVILVKGNETISYSVPADWIEGFKGAAKAFIDAITENESLDMDIDFSSQVLEVA
                     LAMYESSDKEATVYIKESY"
     gene            19376..20830
                     /locus_tag="QF838_08950"
     CDS             19376..20830
                     /locus_tag="QF838_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HJO60845.1"
                     /translation="MASQNTDKPNILFILSDDQGAWAMGCAGNGELSTPNLDRLAETG
                     INFQNFFCASPVCSPARASILTGEIPSQHGVQDFLRKGNSKFINTDGTEITYMEGRTS
                     YIEQLSENGYNCGLSGKWHLGNARLPQFGFEYWNVHASGGGPYYKPPMIENGELYRPA
                     EYVTDIITDNALSFLEKQIDSDSPFSLNVHYTAPHAPWTRENHPQEKFDAFYNESRLE
                     STPDLPIHPHQLDKEAGAATIGSDQEGRKTALSGYFTAIEEMDRNIGRIIDWLEENDL
                     RDNTLVIFTADNGMNMGHHGIWGKGNGTYPPNMYDTAVKIPTLVSRPGHVPQNVDCTE
                     LLSQYDFSPTLLDYVGLEQKNTHNLPGRSFVNLLEGRDFGSDKPVYVLDEYGPTRMIR
                     THKWKYVHRYQFGDNELYDLENDPDEEINLFGSDAHQEIKEKLLEQLESWYDKYTDPS
                     VDGRKNSIMGRGQIGMIKDSDKPFADDVVYFNQS"
     gene            complement(20849..21991)
                     /gene="dgoD"
                     /locus_tag="QF838_08955"
     CDS             complement(20849..21991)
                     /gene="dgoD"
                     /locus_tag="QF838_08955"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HJO60846.1"
                     /translation="MPKPTKIEKLETFLWDRWLLLKIHCEDGTVGIGEGGVHGWQRPT
                     KTMIETMEPYLLGQDPHKIEHHYQWLYRSSHFMGSVVQGALSAIDIALWDIKGKRLGV
                     PIYDLMGGKTRDSVRCYMHVGSGEGGSAEDLANDALSAVKKGYTAVRFSPFSTDYYLH
                     KSYSEWADEAVNRVGAVREMVGPDVDVCVEIHRQMNPAESISLGRRLEQFNPFFYEDP
                     MLPDSPKIMGEIAAKCNIPIATGERFTTIFEYQQLLESNAVSYVRPDLCLCGGLSGSK
                     KVAAMAEANHVKVIPHNPLSPVSTAACVQLDACIPNFALQEYTGEDQPPKSELFVESL
                     EMIDGYLKVPEGPGLGIEINEEALSMPENPKVLDTPIGFDGSVQDR"
     gene            complement(22024..>22459)
                     /locus_tag="QF838_08960"
     CDS             complement(22024..>22459)
                     /locus_tag="QF838_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO60847.1"
                     /translation="RTMFNYAGQGALGMGFSVGLGTKLGRPDRTAVSLHGDGGFLYTC
                     GELNTAVRHNIPSVSIVLNNSCMGAEKSQQKALHNEKYVGVDLENPRFDKLAEVFGAK
                     GYYAEHPSEIADTVAEAVKSNAPAVIEIPVQEYFPPSAPTPR"
BASE COUNT         6930 a         4744 c         4875 g         5910 t
ORIGIN      
        1 cattttctcc aatgatttat tcaagtctgc agatcgggtg tccaaaacga tatgactcac
       61 acctaagttc tcatactctt ttatgtcagc cactatttgt tccggattcc cagaaaaccg
      121 tcgtctgttg ccgccgaaag aattttcttt gtcatacacc ggcgctttta aaactatgga
      181 tatttccgat cggtttctcc cgattttctc acaatatgaa tccatataat ctatctcaac
      241 tgccatttct gaaggttcaa gtggagaagc tgggttagca cctacaggat gccatgcatc
      301 tgccatttta actactcttc gaatagctgc tctactttga cctcccaccc aaatcggggg
      361 gcctggtttt tgtattggct tgggagagaa ttgaagccgt ttgaaagaat agaactctcc
      421 gtcataggaa ggctcctcct cactccacaa ttccttgaaa atacttatat attcgtcagt
      481 aaccttgcca cggcgattaa atggaggtgt atccaaggat tcaaattcct cttcaagcca
      541 gccaacacct actcctacaa ctaatctccc cccagaaagc acatctatgg tagataacat
      601 tttggctgtt aagacgggat tcctatgagg aacaatcatc acgctagtta caagtttcgc
      661 tttgcttgta atcccggcga gaaatgccag ctccgtaatc atttctaaat gctctccaga
      721 actatcccat ggcacctctg acccaacaga gtacgggtac tgggattccg ggtttatcgg
      781 agctatcacg tgatcgcccg caaccattga atcaaaccct atattttcag cgtgagaggc
      841 aatttccaat attggatctc tcttagcaag tggcccatga gtcggcaaat aaaatccaaa
      901 ttccattttc attccctttt aaatatattt ataaagttaa attgattttc aagaatttat
      961 gaaccaaatc attgaaaaac tccggtcgtt cataataaac agaatgccca gctcgatcta
     1021 ccatgatgaa ttccgaatgg gggattaacg aggaagctat ttctatagtt ttagggtgga
     1081 ctaatttgtc ttctcttccc accataaaaa gcattggtat tcccaggtcc ctgaacctgc
     1141 cgtcagtaga accactgtga tttggcctgg gggcaagaaa atccggacta tgtcgtggat
     1201 tcatcctcat gatctgccta tacaataaag atttctcagg aaattgttct gaaaattcag
     1261 gagatagggc cttgattgcc ccttccggta aaaaaggagg cgatgctttg tgttttctcc
     1321 gcacttccct ggactcttca ttatccgatc ctccattagt accagagagg actaatgctg
     1381 acacacgttc cgggtttcta attgcgaata ctgatgcagt acgacctccc atggaatggg
     1441 ccacaatagc cgttttctct ataccaagat ggttgagcaa gccttctaaa tcatctgcaa
     1501 aggcagttct tccttttggc tcagactcat ctggcgtcct accaaaagtc ctatgatcga
     1561 aaatgacaca tttatagtgt ttggaaaagt aggggatttg ttgccaccaa gatatgtgat
     1621 tacctcctga accatgagca aagacaactg gaggcccctc cccgtgtacc tcatagtaca
     1681 ttgagactcc atttacatcc gcaaaagcca ttgttctcct tttggaaagt tttaaataac
     1741 cactattccc ttacacctgt taccccgccg tctatgacca actctatgcc agtgacaaat
     1801 gaggattcat cagaggcaag gtataaaacc ccgtaagcaa cgtcaattga ttgcccaata
     1861 cggccaagtg gtatagatcc aactcttgat tctggatcaa cagaattcgt gaccgattta
     1921 atcatgtctg tattaattgg tccgggatgg acagaattgc atcttatgtt ttcagaggcg
     1981 aatcgcccgg cagtagattt agttagaact ctcacagctc ccttagatgc cgcatatgcc
     2041 ggtgaagaaa gtggccgacc cacaatgcct gctattgagg agatgttcac gatagaccca
     2101 cctccggctc tttgcatggc tggcacggca tatttgatcc ctaaaaacac gcctttggag
     2161 ttaatctcca tcgttcgatc ccaatcctcg gaagaaacgt caaggtaatc ttgcggcaat
     2221 gctatacctg cgttattaac taggatgtct aatttaccgt actcagattc cgccaggttt
     2281 atagctcttt cccaatcctg ggaattggtt acatccaaat gaatatactt cgcattatga
     2341 ccagcagccg caatattttt ctcaacttct aaacccaaat catccaatat atctccaaat
     2401 aaaacttgag ccccctcagc ggcaaaaagg gcaacttctt cagcgccttg acctttcgct
     2461 gcccctgaaa caagggccac cttaccttca agcctattca ttgttacctt ccgatgaatt
     2521 gtctatacag tttcccaagt cgtaacgtca ccatgctgat gtacccttcc gaaatgttcg
     2581 ttgggatgga aatggcccgc tgctatgatc atgccctgtg aaaccgacct ctctataaga
     2641 tcacgtctac ttctttcact agctatctta tcgacgtcta ctccagcaca ccaagatggc
     2701 tcacttattt gtactttgct atgaatcgca tccccaataa tagctgcttt ctgtccgcca
     2761 gaattcaata aaaatgacat atgcccaggg gtatgcccgg gagttattag tgaagttatt
     2821 tcgtcagtga cattaaatcc atcaccgaaa agatccatca aagacagggc cttcaacggt
     2881 acaacatttt cacttaccca gggagcatta attagattct caggcctggt gaaataatcc
     2941 caatctatcg atggaaccaa atatctcgca ttcggaaaat acgggcgagg cggatctgag
     3001 gaaagatcca aattccaacc tacgtgatca atatgtagat ggctatgtac cacgatatca
     3061 atttcttctg gtttaatacc cacccttcca agatcattta ggagatcgcc cttttgccct
     3121 cctcgagtag cgtgcggccc aggccctata cctgtatcaa ccagaactat tttccctttt
     3181 gaccttacaa caaagcatcc atagtaaagc tgaaccatac ctttatctaa aatttcgctc
     3241 ctgtaagggt cccagagtgc tatgtccaca tctggaaaaa aatcctctgg agaccgggca
     3301 ggcgggacca tatccatcag tgataggatc tctaaattgc ctatttgtag tttttcttgc
     3361 attaaatacc cccgactacg cttgcaacca attcttgttt actccaagcc tgataaactc
     3421 gcgccgacat tgtattcttt ttttgcaagg atttatctaa aggtcgacag ggggagaaaa
     3481 tcgatatgga aaaaattaaa gtcggattca tcgggctagg aaacatgggt ggaggcatgt
     3541 ctatgaatgt agccaaagca ggctatgaca tggtagttca cgatataaac aaagccgctg
     3601 caacaaacct tctggaatta ggtgctgaat gggccggtaa ccctaaagaa ttggcagaat
     3661 catctgatgt aatcttcact tcattacccg gtccagttga ggttgaagca gtatcaatgg
     3721 gtaatgaggg attaataaaa ggagtgtcaa acggatccat atggattgat cttagtacca
     3781 gctctcccac cctgattaga gaattatccg aagaattcga aaagcagggc acttctgtat
     3841 tggatgcccc tgtcagtggt ggtgtaaaag gagctgaaac gggtaaattg gcaatcatgg
     3901 ttggtggaag cgaaaacatc ttcaatcaaa taaagccgat tctagattct tttggggata
     3961 aagtaagcta taccggttct attggttctg gcagtatctg taaattgatg cacaacacca
     4021 tcggatatgg gatgcaaatg atcatatctg agtgcctaac tcttggagta aaggctggag
     4081 tcgatccgga aaacttattc gaatgtataa agaatggaag tgtcgggagg ggtaatttac
     4141 tcaatgtcac attacctgac acttacttac aaggtaaatt tgacccaccc aattttgctc
     4201 tcaagttagc aagaaaagat gtttcattag cgctagagct tgggcgcgaa tatgatgttc
     4261 ctatggaggc agcaagtcat gcttacaatg aactaactgc tgcaatgaat agaggatggg
     4321 aaaacaaaga ttccaggatt agcctactcc ttcaagaaga aagagcaggc aatatcgagg
     4381 ttagattgga caactcaaag taggctccaa aagtaaccgg ttaatctccc ggcaccattt
     4441 cagcaaccga ccccttaaag gctggcataa tttcctctga aaatcgttct aattgttcta
     4501 ttattacaga ttttaaggtg ccgactgggt ggctaacact gactctattc aggccgggat
     4561 attttttctc tagtgatttc aattgttcaa taattaaatc gggtgggcca gcaagtacgc
     4621 cgccagcctc gacggcatcc tctatcgttg gcaatccagc cttgagtgct gtttccggat
     4681 tgcccatagc agtaatctgg tcatcactca aagctttcac cagtcggaga gggccaaaca
     4741 tttttagatt ttcttcgtaa aaattaccga cttccttcat ggcttgctct ttcgtgtccg
     4801 caatatgaaa atggaatcct atcgccaaat tctctccgag ttccgtatcc acccctatcc
     4861 tggaataggc gtccctaaat ccttctaccg ctttatccat agcccctcct tcagaacttc
     4921 ctccccctat aattccgctc attccatgct tcgccatgaa atccaatcca cggggtgtgg
     4981 cactttgaat aggttgccag cattcgacag gtcgatttac tggtctcggc accaacgtga
     5041 tttcttttaa ctcatagcct ctgtagggaa cctcgggcgg tatggtgtag taggaacctt
     5101 tatgggaaaa ggaatcctta ttaaaggcct caaatattat atcgacctgt tcctcaaaca
     5161 agtctctgtt ggccggttga tctagaaggg gagaaccaaa ggtttcaact tccctagtgt
     5221 ggtatcccct accaacacca aacactaccc tgcctccagt cagcacatcg gcagtagcat
     5281 aatcttctgc aagtctcaga ggatgccaca taggagcaat attgaacccg caacccaatc
     5341 taatattctt cgttaaatgt gccaaatgta atgccatcat gaggacattt ggcagacatt
     5401 cgtaaccttc aggttgaaaa tgatgttcag ccatccaaaa tgtgtcatac ccacatcggt
     5461 ccatagtttg agctatttct gtagccttat cgaaggctga cacaagatat tcgttaccaa
     5521 atgtccggtc gttcacaggt gtcccggcat acccaacatt tgccaaatct acatgtccgg
     5581 cataaagact atcgaaattg gtaatcattc tcccgctcct tgatgcttaa gaaaattaac
     5641 ttagactttg gcgaataata ccagagaacc agttgtacta tttcaatcaa aaaaagtcct
     5701 ggaaaacctt ttatgagatc cggaaacaaa aaaaatagaa actgaagtaa aatgtcggcc
     5761 attaccaaaa cataaggtac ttatatgacg ctaaaaaaaa tacttatagc taatcgcgga
     5821 gaaattgcaa tccgaattgc aagagccgcc tcagacatga atattacgac tgtttcaatc
     5881 tattctgaag atgatgcttc atcacttcac gttcaatcaa cagatgaatc ttttggattg
     5941 gatggggaag gagtagccgc atatctagat atagaccaag tggtggaaat agctaaaaaa
     6001 tctggtgcag actcgatcca tcctgggtac ggatttcttg cagaaaaccc tggatttgca
     6061 aaaagatgcc aagaagaggg aatcgttttc atagggccaa gtttggaaat gctggagcta
     6121 tttggagata aggggaaagc tagattagct gcgcaaaagt caaacgtacc aacaccagcc
     6181 ggactagaag gtccgatcac acttgaatct gcaaaagatt tttttagcaa ccttgaagaa
     6241 ggtagcggta tgatgcttaa agccatagca ggaggaggcg gaagaggaac tagagcaatt
     6301 aagaatccat ctgaagtaga aaggttattt aagcgatgcc aatctgaagc aaaaagaagt
     6361 tttggaaatg gtgatttata tgccgaacaa cttgtagaaa atgctaggca tattgaggtt
     6421 cagataattg gagataattt tggcaatgtg acgcatctct gggaaaggga gtgcagcata
     6481 caacgccgct atcaaaaatt ggtggaaatt gcccctgcac cttttattac gcaaagtctt
     6541 cgtgaccaaa ttatcgagtc agctatcaac cttgccgttg acgtcgggta ttgtaattta
     6601 ggcaccttcg aatttttagc ttaccaaagt ggtggatctg aatacgactc gtttgtgttc
     6661 attgaaggaa atgccagatt acaggtcgaa cacaccgtta ccgaagaaat aacaggaata
     6721 gatatcgtgc agtctcaaat ccgattggcc gacgggtcta ccttaagtga tataggactt
     6781 gatcccgaca atcctccctc agtgagagga tacgcgatac aaaacagaat taactgcgaa
     6841 actatatcta acacaggtca aataaaaccc tccacgggaa aaattactaa atatggtgca
     6901 cccggtgggc tagggataag aactgacgga tttggctatt caggctatga aaccacaatg
     6961 gcttttgatt ctcttattgc aaaactcata tgccattcaa attccgacaa tttccttgat
     7021 gctgtaaatc gctcaaaacg agcattatcg gagttcgaca tagaaggagt tgaaaccaat
     7081 atcgaatttc atttgaacct tctgtctcac cctgaattta tatcgggaaa tataaccact
     7141 cttttccttg atgataactt ggctgaatta attccagacg catcatcgtc tactggagcg
     7201 gccaacacta gccaaccact caaagccgat gagggatttg caggagccaa tctaaaaacg
     7261 aaggatccct tagctctgtt tgattacgat caacaagtaa aatctactca acaagaatca
     7321 gaacctgaac taacaacacc acaagggcca gatgggactg taaatgtggt tgcacctata
     7381 caaggaactg tcataagcat agacgttgat ctaggacaag aagtaacaaa aggtcaacaa
     7441 ttactagtca tggaagccat gaagatggaa cacatggtca ctagcaacat cagcggaata
     7501 attagaggta taacaattaa tgaaggcgat acaattcggg aaaattatcc tcttttccat
     7561 atagaagaat ctgacgtatc tgtatcttca aaggacctca accaagaatt ggacccagat
     7621 tatattaggt ctgatttgca agaactaaat gaaagacgat cccacatatt tgatgaaaat
     7681 aggcccagtg ccgttgacag acgtcgaagc aaagggcagc gaacggctag agaaaacata
     7741 gccgaccttt gtgatcccga tagttttgta gaattcggtt ctcttgcggt ggcagctcag
     7801 cgactaagaa gagatgaaga ttggctgcga ataaacactc ccgcagacgg gctaatatgc
     7861 ggggtaggta cagttaatgg cgatatgttt acagaaaaag actctaggtg tctcgcagta
     7921 tcctatgact acactgtttt cgcgggaaca caaggtaaca gaaatcacta taaacaagat
     7981 agggtctatg agctcgcaca cagatttaga ttaccagtag tactgtttgg ggaaggtgga
     8041 ggaggtcgtc caggggatac cgataaaatg ggaggtattg gaatggatac caccactttt
     8101 acgacttggt ccagattaag cggacttgtt ccaatggtag gagtcgtctc cgggcgctgc
     8161 tttgcaggaa ataccgccct agtcgcatgt tgtgacgtta ttatcgctgc ggaagacacc
     8221 actatagcta tgggcgggcc ggcgatgata gaaggcggag gtctaggtgt ttatacacct
     8281 gaagaaatag gaccgctgtc ctttcaggtg ccgaacgggg ttgttgatat tctcgttaaa
     8341 gatgaagccg aaggtgtaga agtagcaaaa aaatatatct cttactttca aggtgctact
     8401 gaagaatggg aagaaaatga tcaacgagtt cttagacact cagttcccga aaatagaaaa
     8461 agaatctatg acatgagatc cattatagaa accatcgcag acaaagattc agttttagaa
     8521 atacgaaaag attttggtat aggaatcttc accgctttaa taaggattga gggaaagcca
     8581 atgggattga tcgcgaataa tcctcatcat ttggcaggag cgatcgatag tgatgcctct
     8641 gacaaggccg ccagatttct tcaactctgc gacgcattcg atttgcccat cgtaagcctt
     8701 atggattgcc ccggcttaat ggtggggcct gaagtagaaa agactgccct ggttagacat
     8761 tgcggacgat tattcaacac gggagctaat ttaacggttc ccatgtttgg tgtgatagtc
     8821 agaaaagcat atggcctcgg agtgcaagct atgtgcggtg gaggctcact agtaccattc
     8881 tttacagtag catggcctac cgcagagttt gcaggaatga atattgaagg ggctgtaaaa
     8941 ctaggttatc gaaaagaatt agaagcaatt gaggaccccg aggaacgcat caaagaatac
     9001 gaagggatgg tcgagaaaag ttatgagaga gccaaagcgg taaacgctgg aagttacttt
     9061 gggatagatg acgtcataga tcctgcaaac actcgaaaat ggctcgttgc tggtttaaaa
     9121 agtttaccgc caactcctcc tagaacagaa aaaaaacgtc aatacataga tacttggtag
     9181 accaaatttg cctactgaaa gccaaaaccc tgttcatgcc caagttcttg aaggggtaaa
     9241 aataatagac ctgactggtc cgtctggttt ctattgcacc aaattactag cagatttagg
     9301 cgcggacgta attcgaattg aaaccggatc aactgctgaa cttgaaactt tcgcaccttt
     9361 ctatcaaggt aataaagata tagatagcag tttatatcga tggcatttcc ataccaataa
     9421 acgtagtgtt gaattggatc tcgcttcgca gcagggtaaa gatacatttc ttcatctgtt
     9481 ggaagtggcc gatgtgttaa taacaacgga accacaaaat ttagccaatg caacagtaat
     9541 taagaaagct gaaatacgaa ccaggtaccc acaacttatc catactgtga ttagggcttt
     9601 ctcctccaga agtgcttact cagaatacaa agccacagat attgtggcat tggcgatgag
     9661 tggacttatg gcaattacag gtttcccaga agacccaccc aatcagatgg gtggagagca
     9721 ggcctatcac atggtttcaa tgcatggtgc cataggcaca ctggttgccc tgctagaccg
     9781 tgatttgacg ggaaaaggaa aagacattga agtatccatg caagagtgta cctccatggc
     9841 tactcttcaa acagcaaatc ttaattacta catctggcaa aatctaaacc gtcaacgaac
     9901 cggattgaat catccatttt ctgtccctgc agctgatgaa accctaccga cctacccaaa
     9961 aactttatac aaatgtctgg atggatgggt atcttatgcg gcacatttgg cgccgcctgg
    10021 agcgtgggaa agattcttgc aatggctaga agatcatgac agccaacaag atttaagaga
    10081 tcccaaatat ttggatccag aaataagaca aaaaaaccag aatcacataa atgaggtcat
    10141 ggctgagcat tgttcaaaat taaaagctca ggaactctat cactcagctc aaagctacaa
    10201 gcttctttgt gtccctgtac aaaatgctgc agacctcatt gcagacaaac agcttgagga
    10261 aagaaatttt tttaagactg taaaacatga taattgctca gaagatttca tttatcccgg
    10321 agcaccttat aagttgtcag aaacgccttg ggatattaaa aataatgccc caaagatagg
    10381 acaacatact aaagaagttt tagatgaatg gctcaaccct gcaaatgcaa aggtgagcta
    10441 tgaatagccc tcgtttaccg ttatcaaata taaggatcgc agattttgga tggatgatag
    10501 ccggccccct agcgaccaga gtatttgcca attttggagc tgaagttatc agaatcgagt
    10561 ctggttccag gatagacgaa ataagagttg tcggggcaag accggaagga attgaaagcc
    10621 caaacgttgc tggggtgttt aacgatatta acacgtcaaa gttaagcgtg actttagatt
    10681 taaatactga cgaaggcctt tcattagcca aaaacgtaat cgaaataagc gacgttgtaa
    10741 ctaataattt caggggcgat agaatgggaa aatggggatt agattatgac aatctgaaaa
    10801 aaataaaacc cgacataatt gctctcagta tgccaatgat gggaagtgac ggaccccatc
    10861 aggactacgg aggtaatggt ataaatatta tagctggggc aggaattagc agcataaccg
    10921 gttatccaga caaagcgcca gttggtactg gatctttgta cccagatttt agtggtaacc
    10981 cgaatcatgc ctctatagcc ctattagctg ctatcaggta cagaaacaga acaggtaaag
    11041 gccaatttat agaccttgcc caatatgaat ccaccgtatc tctgctaggg actagcatcc
    11101 tcgaatacac agctaacggt aaattacccg atcggccagg aaacagatct caaaatatgg
    11161 cccctcataa cgtttatcgt tgcaaaggca tggatcgttg gtgtgccata gcagttagca
    11221 ctgaagaaga gtggcaaaac ttaaaaaaag gtctgggcga gctagagtgg atgaatcaac
    11281 ctaaattcgc aacctttaat cagcgcaaag caaatgaaga agaattggac gaaggcatta
    11341 gtaattggac ttcggataaa gatgcatacg aggtaatgaa cctactacaa tcccacggtg
    11401 tacccgctgg cgtggttcaa gatactgaag acttacttaa acgggataaa agtttcactg
    11461 acactcacgt tatcgatatg ccacacccag aaatcgactc aatgaaggtt cacggtgaga
    11521 cgatttcaat gtctggtgtc acacccaaag ttgatagggc cccgctacta ggagagcaca
    11581 ctgaatacat tttaggggac cttttaggag tagctgaatc aaaaatagat gacttatatg
    11641 tttcgggagt acttaaatga ataaggagat agtgatggca aacaaactgg acggcgatgt
    11701 agctatagtg acagggggaa acagcgggat aggcgaagca actgcccacc tgtttgcaaa
    11761 agaaggggcg aaggttgcga tcttagctag aagagagaaa gaaggaaaag aagtcgaatc
    11821 tgctataaaa gcttccggtg gagaagccac attttttcaa tgtgatgtag cggaaagagg
    11881 ccaagtagat gaagtagtcg aaaatgttgt ctcaacatac ggaaaaataa gtgttctctt
    11941 taacaatgca ggaggtggtg ataaaggtaa cttccccgac gaaacagatg aaggttggga
    12001 cagggtcatt gcggtaaatt tgaatgggac attctacgta tgtcgtgctg catggtccca
    12061 tttgatcgaa tcaggaaacg ggcgaatcgt aaacatgtcg tcactagcag ctcaaagagg
    12121 gtttagtaaa aacatgtacg atctggtagg ccgcgggcct tcgtcctcct attacgcagc
    12181 aaaagcaggt gttgatgcat ttaccagata tgcagctggt atgggtggcc aacacaacat
    12241 tagagtaaac ggcgtaagac ctggtcaaat cataactcct ttgaccgatg tcggcggtgg
    12301 aaatcattcc ctcaaagggg ccttcgattt tatccaaata ttagaaggtg caggttaccc
    12361 cgatgacgta gccaataccg ttttattcct agtatcaaac gattccagat ttatcacagg
    12421 agagataata aatgttgatg gaggtatgcc tggaaaactt taatttcgtc aaacgagcat
    12481 tgtttctatg aaccatctat gtgtagtagg atgcgagaat aagagcattt agaacttacc
    12541 aaaaatagtg gtatatgcag tatacattta cagaaaaaga agaagatttc agaagtgaat
    12601 tactggagtt tttagataaa gaattgcctg aagactggaa ttctttatca ccagaaagtc
    12661 atttttctaa ggagaattta ccgtttacca aatccatggc caaaaagctg gcagaaaagg
    12721 gttggttgac catggcatgg cctgaagaat atgggggtca gggtcgctca ataatggagc
    12781 aattgatata ccgagaagag atgacttatc aggatgttcc gggaacagat ctaggaacag
    12841 gtgctattag ctgggtcggg cctaccttga tgattgccgg tacccatgaa caaagaaagg
    12901 aacatctacc tcctattgga aatggcgatg cctattggtg cactctttat agtgaacctg
    12961 ggtcaggatc agatttagcc tcactccaaa cacaagcaca aaaagatggt gacgactacg
    13021 taatcaatgg ctcaaaaata tggaccagct ccgctcatat cgcagacttt gggtggctag
    13081 cggcaagaac taatcctgaa gccccaaaac acaggggtat tagtttattc atggtggata
    13141 tgaaatcacc tggagttacg gtaaggccaa tatacaacat ggccggctct catgaattca
    13201 atgaggtttt ctttgaagat gtcagaatac cttccaaaaa cctagtggga gaagaaaatc
    13261 gcggttggta tacccttgcg gtggctttag acttcgaaag gtcgggagtt ggctattcag
    13321 ccaatgcaag aagaatactt gaatctctga cagtctatgt aaagtcagca cataaaaatg
    13381 gcaacctgct ttctgaaaac caattagttc gaaatagact tgctgaaaga catattgaaa
    13441 cagaagtttc aaggtctatg tcatataagg tcgcttggat gcagagcaac ggtatggtcc
    13501 caaatgctga ggcttccatg tctaaactat ttggaactga gttggtacaa agagtagcaa
    13561 atacggcaat gaacataata gatatgaaag gcctgttatc acatgaatca gatctatcac
    13621 ccttggaagg ggccgtccag aaaatgtatt tgagctcttt ttcatccaca atagctgccg
    13681 gtacttcaga aatccaaaga aacatcattg cagatcgagg tttaggttta ccgagggctt
    13741 agttagatat ctaaatcaca tatggtggtc tccgacgaaa tggaatcttt atgtcctcca
    13801 actcaagaaa ggcttctgcc ttataccatt ttagatctgg ccgaaggagg ccataatatt
    13861 tgcggtcggc tcttgggaga tatgggagcg gaagtcatca aaatagagcc ccctggtggc
    13921 agtcaaaccc gtctcagagg accctttaca aaaaattcag atggtaagcc cagaagccta
    13981 tattgggcat catataactc caacaaaaaa ggtatttccc ttttacttga taccgatgaa
    14041 ggaaggcaaa tatttcttaa gttggtgaac aaaagtgatg caatattaga atcgtttcag
    14101 cctggatatc ttgattcatt aaatatagga tttgaaaccc ttgcaaaaat aaatccccaa
    14161 ttagtgcata cctctatgac gcctttcggc tcaaccggac catatgctgg tttcatatcc
    14221 acggatctaa taaccagttc aatgggcgga atgccttttg taagtggtga cgaggatcgt
    14281 ccacccgtaa gaataagctt tccacaggcc gaattagttg ctggatccca ggcttttgga
    14341 gcaacagtag ctgccttaag gcacagtaga aacagtaaaa ctggacaaca tgtagatgta
    14401 tcggaacaaa tagcggtgat gtggacttta atgaacgcca cgccatttcc tcaattacac
    14461 ggcactgatg taaccagggc gggggcattc agaaaaagag gtcctataga tgcaagacat
    14521 gtatttagat gtgctgatgg atatgtaagt atgaatgcgc aggccaaaac acttgatggg
    14581 tttattaggt ggatgtcaga agaaattgag atcgaaaaag agatcctagg ttttaatatt
    14641 gataaatgga accttaaacc tgatagtgac ccgaatggta aagaagcctc tgagttcaaa
    14701 aaagttgaaa aagctataga gagtttcctt tcaataaaaa caaaaatgga aatttttgaa
    14761 agggctctat ctgctgggct tttggtggca ccttgcaata ccgttgaaga tattagaaaa
    14821 agcccccaat tagaggcaag atcattttgg attgatgttt atcatccgga atttggtaag
    14881 gatataactc atctaggacc ctatatgaag atgtctgaaa ctcctctaaa agtttactct
    14941 ccctccccca gtgttggatc tcataatgaa gaaatattca aaagtcttgg cataggcaaa
    15001 tccgaaatcg aagcgttaag gaagaggggg attatatagt gcaaaacaac acatcagaac
    15061 ttctttttga cggtctaaaa gttttagatt ttacatgggt tggtgtcggc cccatcacaa
    15121 caaaatactt tgccgaccac ggggcagatg taataaaaat agagtctgta agtagacctg
    15181 acgtactaag aggggcaccg ccttttaagg acgcaatacc aggcataaat agaagtcaat
    15241 ttagtggaaa ttacaactca agcaaaagga atctgggagt caatttatca atcccgaaat
    15301 ccgtagacct aataaaagat atcatagttc agtggcaacc tgacgtgata gcagaaagtt
    15361 tcacaccacg cgttatgaaa tcgtggggtc ttgactacct ttctgttaaa agaatagccc
    15421 cagatgtgat ttattttagt acctgcctaa tgggccaata tgggccacat aacaattacg
    15481 caggatttgg gcaactggcg ggagcaatgg ctgggtttta cgaaatcaca gggtggcctg
    15541 ataggggccc cgctggacct tatggggcat attctgactt cctcaaccct cctgtagctt
    15601 ttgggtcaat agtggcagca cttgattacc gggatcgtta cggaaaagga caacatattg
    15661 acctttcaca gtatgagggg gcgatgcact ttctagctcc acatatcatg aaatacaacg
    15721 aggatggctt cattctaggt aggatgggaa acgaggatga agagatgtcg ccgcatggga
    15781 tatttcagtg tctagataag gaaaggggtc tcaccgacac agaggaatca tgggttgcaa
    15841 tagccataga atctgaatcc caatggactg aattttcaaa agttttggat ttaccagagg
    15901 cgatatctaa tcaaaatttg gcagagcgga aattgaacaa agatcaaata gacaatttaa
    15961 tctcgaactg gactttacag aaggatgcta gacaaattat gaatttgcta caaagccacg
    16021 aaatcccatg tggaatggta caaagtcagt ctgatatgtg ggaggatcct caactcaagc
    16081 acacggactt ttttcaatgg cttgaacact cagaggttgg tccgatgcca tacgacggac
    16141 tgcagttcac actgtcaaaa acgccaggga gtctaactcg agcccaggcc atggttggtg
    16201 aacacaattt ggaaatcctt gatgaaattc tttctatggg tccctcagaa gctgcgaatt
    16261 tattaaatga aggtatattg gagcaatcat tttgatgacg aaaggcgcat tagatggagt
    16321 aaaaatcgtt gatttaagca gaatggcccc aggaccattt tgttcgatga tgcttggtga
    16381 tatgggggcc gacgttataa aagttgaagc acctcctaca tccagaataa tagcttccaa
    16441 aatcattaat gacgcagaaa cccgcaaaaa agcggcaagc aacccattaa accgaaataa
    16501 aagaagcata gtattagatt taaaggaaaa agatggaata aaaatattgc atcaactttg
    16561 ccaaaaagcc gatgttttta ttgaaggatt cagacctgag gtagtaacca gactgggttg
    16621 taattatgaa accattaaag agattaatcc ttcaataatt tattgttcta tatccggcta
    16681 tgggcagact ggaccataca aggatttagt gggacatgat gtgaactata tctccgtggg
    16741 tggggcttta ggattgattg gcagcaaaaa tggtacgcct tcaatcccat ataaccttat
    16801 tgcagatttc gcagccggag gtatgaatgc ggcttattca atcgtttgtg ctcttttttc
    16861 cagaacaaac accggcagag gacaaaaaat agacacctca atgtcagacg gcgtcaccta
    16921 cctgttatca gccataccag gagaatattt taattctggg ctaatcccaa agcctggtca
    16981 gatgacatta aatggtggtg tcccttatta caatgttttt gaatgtcagg atggtaaata
    17041 cattagcctt ggctgtatcg aacctaattt ctggaaaaat ctttgctcca cactaaattt
    17101 ggaacaattt gccagcagcc agcacaatga gcatgaatac caattgataa tgcgtgagtt
    17161 agaagccacg tttaaaacca aaaagagaga tgaatggtgg gatatattca gggaagccga
    17221 agatgtggcg gcagcacctg tctatgagtt caatgaggtt ctagatgatc cacacatgaa
    17281 agctagaaac atgttcattg aggcaggtag cgtggatgga gcagtcataa ctcaagtagg
    17341 gtttggttca aagcttacag atactccagg ggcggtccga aaccttggga caattccagg
    17401 ggcacactct gaaaatatcc taagagaaat tggctactct gccgatgaaa tcaaaaaaat
    17461 ggtcgactct gaagtagtat tcacctagca ctgatcactc ggctgaaagg tcagggaata
    17521 aggtccctat aagtggccac cactgttccg cacagaacca ggggtaatgg ctcatcctac
    17581 cgctccagta aagagcattt tcgacatttt gcagacgcgc tagagagata gagtgtaccg
    17641 ggtggtccgg caaacaccaa cagtggaaac tatccacatc acatttagat gaaacaacgc
    17701 ctgggaagtg agatacctag gccctccaga tttacaaggg aagaaaaagc acatatatga
    17761 aacttgtcgc ggttctagga gagatgccga gcagctttta gggcaaccct tataactcct
    17821 caacaatttt gtctggtatt agtttaaata tcacgacgtc ttttttatca tcagggtata
    17881 gatcagcaaa ttccctaccc tcctcttctc cgtaatatcg tatagccaat ttttttacaa
    17941 aatcctgaag cggtattttt atttcttcaa cttttccatg tataaccacc gatttgtatg
    18001 gaggttccct gaaatctaca cataaagtag cgtatggatt atgagataaa tttttccatt
    18061 tcaatgtatt agtgcccgta aacatagagg cacaatgacc atcccatcca taccatatcg
    18121 gaaccgaatg cggacgataa tcggccccta cggtactaat aacggctatt tgggtttgat
    18181 ttagaaatac gtcagcctct tcgcttatga aaaccataat tcgccacctt agttaaattt
    18241 aaatgctgtt gccataaata gtaatatgac acaaagagta aaaaataatc caactaaatg
    18301 aaaggtgggt gttatgtcta aagtcggatt agctatagtc ggttgcggaa caatttctca
    18361 actcaatgtg cccgggtatt tggaacatcc aaattgcgag atagtcgcct tatgtgacgt
    18421 taatgaagat agagcaattg caaaggcaaa agagtggaaa ataaaccctc gtatttatac
    18481 tgactacgac agtgttttgg ccgatgaggc cgtagatgct gttgaacttt tatcgccaac
    18541 ccatcttcat gcatcgcaga ttatttctgg tctcaatgca ggaaaacatg tgtcatgtca
    18601 aaagcctata gcaacctcta tggatgaggc aaaacagatt cgcaaagctg ttgaaaataa
    18661 tgaaaaaatc tttagagtga ctgaaaactt tctttattat cctcctataa caaaggcgaa
    18721 ggaacttata gagaatggtg ccattggaac tccttctctg gttagaatca gaactatcag
    18781 gggactgggt atggaaacca tgattaggcc tgaaaaagat gcgtacgtat ggagaaggga
    18841 tccttcaaaa aatccaggcg gaatgctcta tgacgatggt tggcacaaat atgctacggc
    18901 tatatcttgg gttggaggcg ttgaaaaagt aacaagcatg gtaacaagaa ccgatgattt
    18961 tcttgctgat actccaagtg ccgctgttat gaaagtaaaa gacaaagatt gtttaataac
    19021 cattgattat tcttctgcaa atgaaatgcc cattaagggg aaatattaca gtgctgacga
    19081 atttttcgag attataggtc cgaaaggcac aatctgggtc acgagatgta cgggcgaact
    19141 actagacatg cctccggtta tattggttaa aggtaacgag accatctcat acagcgtccc
    19201 tgctgattgg attgagggat ttaagggagc ggcaaaggca ttcatagatg cgatcacaga
    19261 aaacgaatca ttagacatgg atatagattt ttcatctcaa gtgttggagg tcgccctggc
    19321 aatgtatgag tcatctgaca aggaagccac tgtatacata aaagagagtt attaaatggc
    19381 aagccagaac actgataagc caaacatatt attcatattg agcgacgacc agggggcctg
    19441 ggcgatgggg tgtgcaggta acggtgaact gtccacacca aatctggatc gcctagctga
    19501 gacgggaata aattttcaaa attttttctg cgcttctcct gtctgttccc cagcgcgcgc
    19561 ttcaatatta acgggtgaga ttccttccca acatggagtt caagactttt taagaaaagg
    19621 aaactccaaa tttattaaca cagacggaac cgaaataacg tatatggaag gtcgaacctc
    19681 atatatagaa caattgtcag aaaacggtta caactgcggg ttaagcggga aatggcacct
    19741 cggaaacgct cgattaccgc agtttggttt tgagtactgg aacgttcatg cttctggtgg
    19801 aggcccttat tacaagccac ctatgattga aaacggcgaa ctttatcgac cagccgaata
    19861 tgtaacagac ataattactg ataatgccct ttcgtttctt gaaaaacaaa tagattctga
    19921 ttctcctttc tctcttaacg tacattacac ggcccctcac gcaccttgga ctcgtgaaaa
    19981 ccatccacaa gaaaaatttg acgcctttta caacgaaagc cgattggaat ctacacctga
    20041 tcttccaata cacccgcacc aactagacaa agaggcggga gctgccacta ttggaagcga
    20101 ccaggaaggt aggaagactg ccttaagcgg atatttcact gccatagaag aaatggatag
    20161 aaatataggg cgaataatag actggcttga agaaaacgat ctcagagaca acactcttgt
    20221 catattcact gctgataacg gaatgaatat gggacatcac ggcatttggg gaaaaggtaa
    20281 cggaacatat ccacctaata tgtatgatac cgctgttaaa attccgaccc ttgtttcaag
    20341 acctggtcat gttccccaaa atgtagactg caccgaacta ttaagccaat acgatttcag
    20401 tcctacccta ttggactatg taggcctaga gcaaaaaaat acccataatc ttccagggag
    20461 aagttttgtt aatctgctgg aaggaagaga tttcgggtca gataaacccg tctatgttct
    20521 tgacgaatac ggaccaacta gaatgattcg tacacataaa tggaaatacg ttcacaggta
    20581 ccaatttggg gacaatgagt tatatgattt agagaatgat cccgatgaag aaatcaattt
    20641 atttggatca gacgctcatc aagagattaa ggaaaaatta ttagaacaac tcgagagttg
    20701 gtatgacaaa tatactgatc ctagtgtaga cggtaggaaa aactcaatta tgggtagagg
    20761 tcaaattggc atgataaaag acagcgacaa acctttcgct gatgacgtcg tttatttcaa
    20821 tcaaagttaa tataacgtac caataaccct atctatcttg taccgaacca tcaaagccaa
    20881 taggagtgtc cagcaccttg ggattttcag gcatcgacaa ggcttcttca tttatttcaa
    20941 ttcctagacc tggaccttca ggtactttca ggtacccgtc tatcatctcc agtgattcca
    21001 caaaaagttc gctcttgggc ggctgatctt cacctgtgta ttcttgcagg gcgaaatttg
    21061 gaatgcaggc atccaactga acacaggccg cagtacttac tggagacaat ggattgtggg
    21121 gaataacttt tacgtggtta gcttcggcca tggcagcgac ttttttcgat ccgctgagtc
    21181 cgccacacag gcacagatct gggcgaacgt acgaaacagc gttggattcc aaaagctgct
    21241 gatactcaaa aattgttgtg aatctttctc cagttgcaat aggaatatta catttggcag
    21301 ctatttcacc cattatcttt gggctatcag gaagcatcgg atcttcataa aaaaatgggt
    21361 tgaattgctc caatctccta cctaaggaaa tgctttccgc aggattcatt tggcgatgta
    21421 tttcaacaca tacgtcaaca tcaggtccga ccatttcacg caccgcccct accctattaa
    21481 ctgcttcatc tgcccattca ctatatgatt tgtgtagata ataatcagtt gagaaaggcg
    21541 aaaatcttac agctgtatat cctttcttaa cggcacttaa agcatcattt gccaaatctt
    21601 ccgcactacc accctctcct gatccaacat gcatatagca acgaacacta tccctcgttt
    21661 taccccccat aagatcataa attggcacgc ctaatctctt tccttttatg tcccatagcg
    21721 ctatgtctat ggctgacaag gctccttgaa ccaccgaccc catgaaatgg gaagatcgat
    21781 atagccactg ataatgatgt tcaattttgt gtgggtcctg gcctagcaga tagggttcca
    21841 tagtttctat cattgttttc gtgggtcgct gccacccatg cactcctccc tcgccgatgc
    21901 caacagtacc gtcctcacaa tgtatcttca acaacaacca tctatcccaa agaaatgttt
    21961 ccagtttttc gatttttgta ggttttggca cgcttctctc ctttttacaa tcaacgacga
    22021 tttctagcgg ggggtaggtg cggaaggagg aaaatattcc tgaactggaa tttcaattac
    22081 tgcaggagcg ttacttttca cggcttcagc aactgtatcc gctatctctg atggatgttc
    22141 agcatagtaa ccctttgccc caaacacttc agctaatttg tcgaatcttg gattttccaa
    22201 atcaaccccc acatatttct cgttatgcaa ggccttttgt tgagatttct cagcacccat
    22261 gcaactattg ttcaaaacaa tactgacgga tggaatattg tgtctaacag ctgtgtttaa
    22321 ctccccacag gtataaagaa aacctccatc accatgaaga cttactgccg tcctatctgg
    22381 tcttcccagt tttgtcccta agcccacact gaatcccata cccaacgcac cctggccggc
    22441 gtaattgaac attgttcta
//
LOCUS       DASCNS010000248         1740 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001871544, whole genome shotgun sequence.
ACCESSION   DASCNS010000248 DASCNS010000000
VERSION     DASCNS010000248.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001871544"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1740
                     /locus_tag="QF838_08965"
     CDS             <1..>1740
                     /locus_tag="QF838_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012602.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJO60848.1"
                     /translation="PIFNIAMDSCILCGRCAQACQTGHQFIGAIDVLGTSNQTRIGTF
                     MDKPLLESICTTCGQCLSVCPTGAISTKEEPAVIERTVTTTCPYCGVGCGIKAQVTSD
                     DTIVEMLDDPENDSSLGMLCVKGRFGYTFVHHEDRLTEPLVRKNGSLQRVTWDEALDF
                     IADKLAAYEGDSFATLCSAKATNEDGYIQQKFSRLVMQSNHIDHCTRLCHSPSVEAML
                     TSLGSGATSNSYIDYEEAGCLVIIGSDANSNHPVAASRMRRAVVERGAKLIVINPRRI
                     DMCDYADIWLRPRPGTDVALLNGMANVILENGLADEDFIKTRTEGFDDWSDIVNKYTP
                     EYVEAITGVAAADIIDAANMYATPPFSGSCLIWGMGITQHTMGTANAHGLLNLAFATG
                     QLGKPGSGISPLRGQNNVQGCGDAGCLPNAFPGYQTINADSVEKFSTAWGGNPLPDKA
                     GLVVTDMVREIDNGRIKAMYITGENPLLSEPDLNHAEESFKKLELLIVQDIFLHETAQ
                     IADVVLPACSFAEKDGTFTNSERRVQRVRETIKPVGSSRADWEIICDLARRISAKKSL
                     GLGDQFEYRDASAI"
BASE COUNT          501 a          342 c          433 g          464 t
ORIGIN      
        1 ccaatattta atatagcaat ggactcttgc atcctttgtg gtaggtgtgc ccaggcatgc
       61 caaacaggcc atcagtttat tggggctata gatgttttgg gaacatccaa ccagactaga
      121 atcgggacct tcatggacaa gccattgctg gaatctatct gtaccacctg tgggcaatgt
      181 ttatcagtat gtccaacagg tgctatttcc accaaagagg aaccggcagt tatcgaaaga
      241 acagttacta ccacatgccc ttactgtggg gttggttgcg gaataaaggc acaagtaaca
      301 tcggatgaca ccatcgtcga aatgctggat gatccagaaa atgattcaag tttggggatg
      361 ctatgtgtaa aaggaagatt tggatacaca tttgttcatc atgaagatcg tctgactgag
      421 cctttggttc ggaaaaatgg gtcgttgcag cgtgtgactt gggatgaggc tttggatttt
      481 atagctgaca aattggcggc ttatgaaggt gatagttttg ccacattatg ttctgcaaag
      541 gcaaccaatg aggatggcta catacaacaa aaattctcac gtctagtcat gcaaagtaac
      601 catattgatc actgtacaag gctttgccac tcaccatcag tggaggccat gttgacttct
      661 cttggaagtg gggcgactag caattcgtac attgattatg aagaggccgg gtgccttgta
      721 attatagggt cagatgctaa ttctaatcat cctgttgccg cctccagaat gcgaagggct
      781 gtagtagagc gtggcgcgaa actcatcgtc ataaatccac gaagaataga catgtgcgat
      841 tatgcagata tttggctcag gccaagacca ggaacagatg ttgctttgct aaacggtatg
      901 gccaatgtga ttttggaaaa cgggctagca gacgaagatt tcataaaaac tcgaactgaa
      961 ggatttgatg attggtcaga tattgttaac aaatacacgc cggaatatgt ggaagctata
     1021 acaggagttg ctgcggcaga tattattgat gccgctaaca tgtatgccac tcctccattc
     1081 agtggctcat gtcttatatg gggtatggga atcacacaac atacgatggg tactgcgaac
     1141 gctcacggcc tattgaattt ggcgtttgct accgggcagc ttgggaaacc aggttctggc
     1201 atttctcccc ttcgtggcca gaacaacgtc caagggtgtg gcgatgcagg atgtttaccg
     1261 aatgcattcc caggctatca gacaataaat gctgattctg tagaaaagtt ttccaccgca
     1321 tggggcggta atcccctccc tgataaagca gggttggtag taactgacat ggttcgtgaa
     1381 attgataacg gacgtattaa ggccatgtat atcactggtg aaaatccact tctaagcgaa
     1441 cctgatctga atcatgccga ggagtccttt aaaaaactcg aattattaat tgttcaggac
     1501 attttccttc atgaaacagc gcaaatagca gatgttgttt tgccggcatg ttcctttgca
     1561 gaaaaagacg gaacgttcac aaatagtgaa agaagagttc aaagggtcag agagactata
     1621 aaaccagttg gctcctcgag ggcagattgg gagataattt gtgatttagc tcgacggata
     1681 tcagctaaaa aaagtttggg tctaggagat cagttcgaat atagagacgc ctctgcaatt
//
LOCUS       DASCNS010000249         2417 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001871938, whole genome shotgun sequence.
ACCESSION   DASCNS010000249 DASCNS010000000
VERSION     DASCNS010000249.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001871938"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            120..860
                     /locus_tag="QF838_08970"
     CDS             120..860
                     /locus_tag="QF838_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60849.1"
                     /translation="MATFDMKPVEISQEILDGLKKIPTATIYNALRSFGSTFCVCEGI
                     QNYTPFTPGKDRFAARARTLRFMPIRPDINNDKPGGVDSPEYIAMGRCGPGDVLVADI
                     GGRVQDVVAGDVKLLQLAMNNADGVVIDGAIRDLDVLIDEEYDLTIYAKARSFHGNPS
                     SLPAEENVQIQCGGALVRPGDIMVGDNDGVLVIPSWMAQTVLEWAEEHEGAENLVKDK
                     IKAEGVAPGKYYPPTEETIEEWRKLNNR"
     gene            909..1250
                     /locus_tag="QF838_08975"
     CDS             909..1250
                     /locus_tag="QF838_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HJO60850.1"
                     /translation="MKTFAMALDLMDEAGSIEEYKEYHKNVWPEVKEGLLEIGIEGME
                     IYLLGDRLFMVVDTKDDFDITTDFQKYTNSSDKAAQWDILMRKYQKKTPFTEDNDWWA
                     PMQRVFDLNPQ"
     gene            1258..2091
                     /locus_tag="QF838_08980"
     CDS             1258..2091
                     /locus_tag="QF838_08980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60851.1"
                     /translation="MEIVDSHHHLWDLDLFQYEWMPEDNTILRRSYLPSDLASILEQC
                     SVSGSVVVQADQTVEEAKFLLECANDFPWIRGVVGWVDLQDEKVGDTLDELIKLGPLV
                     GIRHQVEEEVDENWLLRKQTLDGLKELSDRNLSYDLLVKPIHIDQIPIVCKELPELRM
                     VVDHIAKPNIRDRVFQSWSDSISKLQPYSNLFCKVSGMVTEADHNNWTPQDLRPFIQQ
                     VRKVFGIDRLMWGSDWPVCLLAGNYQEVMDAALFGMGDMSPQEKKRFLSGTAAEFYRL
                     E"
     gene            complement(2179..>2417)
                     /locus_tag="QF838_08985"
     CDS             complement(2179..>2417)
                     /locus_tag="QF838_08985"
                     /inference="COORDINATES: protein motif:HMM:NF023174.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HJO60852.1"
                     /translation="PNSEAAVNYQKWYEGNDEVKVLGVWGYNVSHTIFGVREAENIQA
                     ITNLLRPQMHTGNVEVLPVMDSIEIRKKGGHWGK"
BASE COUNT          709 a          453 c          595 g          660 t
ORIGIN      
        1 gatactagtc aaatggattg gtggatatgt aattttcata acatatgatg gaagaagtca
       61 gagctacatg ataatgttgc ttacccaaga tagattactg ttctgatagg gggaaattaa
      121 tggctacctt tgacatgaaa ccggttgaga tttctcagga aattcttgat gggttaaaaa
      181 aaattcctac ggccactata tacaatgcgc ttcgatcgtt tggatcaact ttctgtgtat
      241 gtgaaggtat ccaaaactac actcccttta ccccgggtaa ggaccgtttt gcagctagag
      301 ctagaactct gagatttatg ccgatccgtc cagatatcaa taacgacaag cctggaggcg
      361 tagattcccc cgaatacatt gcaatgggac gttgcggacc tggtgatgtg ttggtcgctg
      421 acataggcgg tagagttcaa gacgtcgttg caggcgatgt gaaattactg caattggcta
      481 tgaataatgc tgacggggta gtcatcgatg gggcgatcag ggatttggat gtactcatag
      541 atgaagaata tgatttgacc atatatgcca aagcgcgaag ttttcatgga aacccatcga
      601 gtcttccggc tgaggagaac gttcagatcc agtgcggtgg agccttggta cgacctggtg
      661 acattatggt tggagacaat gacggagtcc tagttattcc ttcttggatg gcgcagacgg
      721 ttctagaatg ggctgaggag cacgaaggcg cggagaacct tgtaaaggac aagataaaag
      781 ctgaaggggt cgctccaggt aaatactacc ctccaactga agaaacaatt gaagaatgga
      841 gaaaactaaa caatcgatag ttgaagtggt gaatttcgtt cacgtattta acgaaaatgg
      901 tgacccaaat gaaaactttt gcaatggcat tggatctgat ggatgaggct ggatcaatag
      961 aggaatataa ggaatatcat aaaaatgtct ggcctgaggt aaaggaaggg ttattggaaa
     1021 ttgggattga aggtatggag atttacctcc ttggagatag gttgtttatg gtagtggata
     1081 ctaaagatga ctttgacata acaacagatt tccaaaaata taccaactcc tctgacaagg
     1141 cagcacagtg ggacatattg atgaggaaat accagaaaaa gactcctttc accgaagata
     1201 atgattggtg ggctccgatg cagagagttt ttgacctaaa ccctcaataa ggcctccatg
     1261 gaaattgtag attcacacca tcacctatgg gatctagatc tttttcaata cgaatggatg
     1321 ccagaagata acactatttt acgtcgaagc taccttccct cagacctagc ttccattttg
     1381 gaacagtgtt ccgttagtgg cagtgtggtc gttcaggctg accaaactgt tgaagaggct
     1441 aaatttctac ttgaatgcgc aaatgacttt ccctggataa gaggagtggt tggatgggtc
     1501 gacttacaag atgaaaaagt cggagatacc ttggacgagt tgataaagtt gggacccctt
     1561 gtgggaataa gacatcaggt ggaagaagaa gttgatgaga attggctact cagaaagcaa
     1621 accttagatg ggttgaaaga actgtcggat cggaacttat cctatgactt attggttaag
     1681 ccgatccata ttgaccagat acccatcgtt tgtaaggaat tgcctgaatt gagaatggtt
     1741 gtggaccata tagccaaacc aaacattcgt gatcgagtgt ttcaatcttg gagtgatagc
     1801 atctcaaagt tacagccata tagtaatctt ttttgtaagg tgtctgggat ggtaaccgag
     1861 gcagaccaca ataactggac tccccaggat ttacgcccat ttatacaaca ggttaggaag
     1921 gtttttggca tagataggtt aatgtggggt agcgattggc ctgtttgtct actcgctgga
     1981 aattatcagg aagtgatgga cgccgctctt ttcggcatgg gagatatgtc accacaggaa
     2041 aagaagaggt tcctatcggg tactgcagct gaattttata gactggaata agggcttcat
     2101 atatcgagta ggtaccgcat ccccatatac aagggggata cggtactggc atataggatt
     2161 ggtatcagat taaaaagtct atttacccca atgcccaccc ttctttctga tttcgatact
     2221 atccataaca ggtaacactt caacatttcc agtgtgcatc tgcggcctca gcagattggt
     2281 gatagcctgt atattttcag cctctctgac gccgaatatg gtgtgagaaa cgttgtatcc
     2341 ccatactccc aatactttga cttcatcatt tccttcatac cacttctgat aatttaccgc
     2401 ggcttcgctg tttggac
//
LOCUS       DASCNS010000250        10701 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001873632, whole genome shotgun sequence.
ACCESSION   DASCNS010000250 DASCNS010000000
VERSION     DASCNS010000250.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001873632"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="QF838_08990"
     CDS             complement(<1..194)
                     /locus_tag="QF838_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60853.1"
                     /translation="MVGVDVGITLTVWVGGGIAVGPVTGVDAGITLTVWVGGGIAVGP
                     VTGVDAGITLTVWVGGGIAVG"
     gene            172..2643
                     /locus_tag="QF838_08995"
     CDS             172..2643
                     /locus_tag="QF838_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60854.1"
                     /translation="MPTSTPTMGDSGALAKDEGDDEGSIESTKAGNFILQCIPGTNDP
                     LNTWVKAKGPIGGLGYNVRYRHDNPDIMYFTDVWSGVQKSINGGLNWTQSNGEGEGAI
                     DFRVGPTLDNIPVYALRIDPNDEDIVWIGLQDNGGLYKSNDGGQSWESKSNGLDPTKI
                     IPQQKLLNADEDEEEDVPELHLTVRMIEIEPGNSNVVYVMGDVDTQVDGFQFNRTRGF
                     VFKSTDGGEQFELVGDFANLTRWLFFTNSDDNEELLVTTGIFDREPDTAVEKEYRNLS
                     DVDAIASGLGVGVFKSNTGSNTWYQSNEGINPKRSMHFGGADQSPFDAKLIIIASGND
                     VDNSLRDYPGAIYKSIDRGASWKDVSPLYSGLGMYGAVAFAESDNNIVYVAGENEFLR
                     SIDQGETWTIHEQWGPAGIKPGNPIDMVVSKTDPNTVFVNNYGGGVFKTEDGGETWIN
                     WSNGYSGANILGISVGRTGCLAVNGRSQIHLSGDFGETWQGILYGKSYSLGDGLAIKF
                     LTHDGSNETIIATDANNGHLYFSSNLGSDWEIATGLVEIEHSLFEESFGIHIVEEAAS
                     NPMVLYAGYIQSKIKGQDPHQLDEIKQSPGMYKSVDGGMSWFPINNGLPDEDYSRNVS
                     DIAVSSQNEDVVYINLLFDGLYYTVDGGESWKQLKGKLPVGMSWHDCGHTCVNEDGER
                     VDMEDMEQSEMIQRKHAMSIAVNPQNDQEIFLGTNVHGVYKSIDRGASWEETLWRTEA
                     INTSKRDHAHAIDISINPANPNTIAVADWNSGVLLSQDGGESWQRTNQGLDSGVVQVL
                     TFSPNGQYLFAGTEGHGIFRYKFFD"
     gene            complement(2908..4104)
                     /locus_tag="QF838_09000"
     CDS             complement(2908..4104)
                     /locus_tag="QF838_09000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO60855.1"
                     /translation="MAIEIRYIQKAEIFESHIAVERANGGHGDKAALPLITEALNDGR
                     GVAAFDEGIIVGFGIAYPLKMNVGIERLPTTLLDNVAVLPTHRRRGILNRMMDFHMRD
                     FYERGETLSALGASESIIYGRYGYGIGSRQVDFSIDRRHTAFINDVSTKGKYSFINSG
                     DALDTLPEVAERANAKRSGFIKGTKSLWKLYLSDPEYRRGDASELFHVVYEENGQVDG
                     YVSYRIKKDILLIHEMISATSTSHTALWRYCFGVDLMRRIDAPKRPIDDPLPWMLSDP
                     RRLHQSLRDDLWLRLVAVKDALSERSYDCEGRLVFEINDRFCPWNENFYELEGSPQGS
                     SCVLTSKSSDISISAADLSSAYLGGITFTALARAGRAHENRQGSLALADNMFKTDLQP
                     WWPNEF"
     gene            4296..4703
                     /locus_tag="QF838_09005"
     CDS             4296..4703
                     /locus_tag="QF838_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60856.1"
                     /translation="MRTVDGGVPLYRVERAFSISRSLLPSLRSDRKLEPLKIIGHKPS
                     LFLFTMTKGNINIASTETPSRAEVDAHEPPVESSAISPCPSGCVSSSPVALPTRLNLL
                     LLLDDLLAVVFFSSSFEGELLNPKKLPKVLLFP"
     gene            complement(4784..5920)
                     /gene="dnaN"
                     /locus_tag="QF838_09010"
     CDS             complement(4784..5920)
                     /gene="dnaN"
                     /locus_tag="QF838_09010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HJO60857.1"
                     /translation="MQVSCLQQNLSRALAIVGRAVATRSNLPVLQNVKIATEDGMLVL
                     TATNLDIAITTKIGAQVETEGEITIPARLLSDFVNSLPDDRIEIKSVVEPLSVSLDCQ
                     RFSANINGTDPDEFPPIPTVDEGATIKVDPQLLKDTVTHVAFAAATEDSRPVLTGIKV
                     EVNQSDFTFAAADGFRLAVYEGKLLEPVSEAMEFIIPARALQEVGRLIGTDDTAVEFT
                     VTPAGTHALFNIGNVEIVSQLMPGSFPNFRSLIPAEYKNRVIVQNSDFSRAVKTSSIF
                     ARDGSGILRIQIMNDDDGGKLSISSRAEEVGDNQGEIDGGVEGDVDEESRIAFNSKYL
                     AEVLDVLGEGEVAFEMTSASSPGVVRATGKEGYTHVVMPMFVQW"
     gene            complement(6045..6935)
                     /locus_tag="QF838_09015"
     CDS             complement(6045..6935)
                     /locus_tag="QF838_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016176.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HJO60858.1"
                     /translation="MNIVKLLGPACIIPILSIGLLGCSSDQVDETPRKAKTPEVTSAP
                     LKQLTVEPAAASVDQKPYEVKKTAPAATPDSPIRTTPMIATVPPNPVPTTKPIPTKPL
                     SSVATSSKGAATPTTAKLESVLISLQPGSVARYRIGEQFARLPTPITAVGETSGINGK
                     VHFNPSGNISSSDASTIVIDVESLKSDESKRDNWVRRNGGIGSEISISLTKVSGHTWP
                     LPDFGKMEVTIEGDMTISDVTNPVIWKGTLDIAESDITGSISTIITWDQFSLSKPRLP
                     FIISVDDEIILELEVVAAKE"
     gene            complement(6977..7480)
                     /locus_tag="QF838_09020"
     CDS             complement(6977..7480)
                     /locus_tag="QF838_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024864.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HJO60859.1"
                     /translation="MIRGLVVSVVMLGLLFGTYACGENVPPKGSHDEVKPAPIVSTSS
                     NQSSSVAVTAPPPATTSSQSSSAASVSAAAPSDYSLDVAMNDANAIYIFNPTDITVKK
                     GETVTLNLTSENEFHSFTVDSLGIDVEVDAGTTEEITFTFSEAGTFDLICIPHESLGM
                     IGKIIVQ"
     gene            7685..8329
                     /gene="lexA"
                     /locus_tag="QF838_09025"
     CDS             7685..8329
                     /gene="lexA"
                     /locus_tag="QF838_09025"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HJO60860.1"
                     /translation="MKRPLSKRQQTILNYIKDFMGNNSYPPTVRDIQTGCEISSTSVV
                     DYNLQILQREGYLQRRREVSRGIELLGETATSTLRNLISIPVVGNIAAGEPLPLVNTE
                     QWNDTDFDNVDLPPFLTKGKENVFALRVKGESMVDALVGDGDLVLLESIEQPQNGDMV
                     AAWLKDREEATLKHFFLEGDKVKLVPANSYMQPITVAAANVSVRGRVVGVVRTM"
     gene            8449..>10701
                     /locus_tag="QF838_09030"
     CDS             8449..>10701
                     /locus_tag="QF838_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HJO60861.1"
                     /translation="MDNNLIDTSLTIPIVGMTCAACVSHVGAALEGIPEVDRAVVNLA
                     TEKATVHISSGHIQKELLKSTVQDAGYQLGSESITLSVEGMTCAACVSHIESSLKKLE
                     GVEEAHVNLATETAKIQYIPGLESISSIRLAITNSGYSSSYIEQEELGYGSTNRAQKR
                     IVQQAIVSLLGAGFIMLGMVSSLASQLPFQSEYLSFIIASIVQFWAGRQFYTSAWNAT
                     KHRTTNMNTLIAVGTSTAYIYSTFAILGKLFGLSDAFPETYFSTSCAIIGMVLLGRYL
                     ESKSRRRATDAIKSLLDLAPKTANIMKGPDVKTVSVDDLVIGDIVIIEPGEKIPADGS
                     VVEGFSEVDESTLTGESVPKAKKMGDPVFTSTINGTGNIKVITESLSDGTVYSKIVSL
                     IEDTQASKAPIQRIADRLATLLVPAILIVAALTFLTWIVLGPDPTYAYATIAAVAVLV
                     IACPCAMGLATPIAIMVGSGRGAENGILYRDALAIETLQKVDSVVFDKTGTLTKGQPQ
                     VTGVDSHSSYSETEILEYAGAVEKNSEHIIAKAIVAECEFRKIVSADASEFRSYPGLG
                     TSGVLNEKIILVGNKNFLREADIEVPEVFNSADLGGVHVSINGSYSGSIFFSDTIKDD
                     AAETIAHLHELNLNLVLLSGDREELVESVANIVNISSYESNFLPQDKAAYISTMQKNG
                     QVVCMVGDGVNDAPALTQADIGIAMGGGTDIAIESAQITILGNRLSSIVQSIRLSKSC
                     ITTIKQNLFWA"
BASE COUNT         3074 a         2229 c         2437 g         2961 t
ORIGIN      
        1 cctacagcaa tcccgccacc aacccaaaca gttagagtta tgcctgcatc tacccctgta
       61 actgggccta cagcaatccc gccaccaacc caaacagtta gagttatgcc tgcatctacc
      121 cctgtaactg ggcctacagc aatcccgcca ccaacccaaa cagttagagt tatgcctaca
      181 tccacaccaa ccatgggtga ttcaggtgct ttagctaagg atgaggggga tgatgagggt
      241 agtattgaat cgacgaaggc tggtaacttt attttacagt gtataccggg tacaaatgac
      301 cctctcaata catgggtaaa ggctaaagga ccaattggag gtttggggta taacgtccga
      361 tataggcacg ataaccctga catcatgtac tttactgatg tgtggtcagg agtacaaaaa
      421 agtatcaacg ggggcttaaa ttggacccag tctaatggag aaggagaggg ggctatcgat
      481 tttcgagtcg gtcctacatt agataatata ccggtatatg ccctcagaat agatccgaat
      541 gatgaagata ttgtatggat aggcctgcaa gataacggcg gtctctacaa atcaaacgat
      601 ggagggcaat cttgggaatc aaaaagtaat ggcttagacc caactaagat tattccgcaa
      661 caaaaattac taaacgcaga tgaagatgag gaggaggatg ttcccgagtt acacttaacc
      721 gttagaatga ttgaaattga accaggtaat tcaaatgtcg tttacgtgat gggcgacgtt
      781 gacactcaag tagatggatt ccagttcaat agaactcgag gatttgtatt caaatctact
      841 gacgggggtg agcaatttga actcgttggt gatttcgcaa atctaaccag atggcttttt
      901 tttacgaatt cggacgacaa tgaagaatta ctcgttacca caggtatatt cgaccgtgaa
      961 cctgacacag cagtagaaaa agaatataga aacctttcgg atgttgacgc aatcgcaagt
     1021 ggacttggag taggcgtctt taaatcaaat accgggagca acacctggta tcaaagtaat
     1081 gagggaatta acccaaagcg gtccatgcat tttggtggag cagatcagag cccatttgat
     1141 gcaaaactaa tcattatcgc aagtggcaac gacgtcgata actctcttcg cgattacccg
     1201 ggagcgattt ataaatctat agatcgagga gcttcctgga aggatgtatc acctctttat
     1261 tcaggattag ggatgtatgg cgcggtcgct tttgctgaat ctgacaataa tattgtgtat
     1321 gtcgctggtg agaacgaatt tcttcgttca atagatcagg gtgaaacatg gactatacat
     1381 gagcagtggg ggcctgcagg aataaagccc ggaaatccaa ttgatatggt agtcagtaag
     1441 accgatccta atactgtatt cgtcaataat tatggtggtg gtgtttttaa aactgaagat
     1501 ggtggagaaa cttggatcaa ttggagtaat ggttattcag gtgcaaacat cttaggcatt
     1561 tctgttggcc gtactggttg cttggctgta aatgggcgaa gtcaaataca tctttctggt
     1621 gattttggcg aaacatggca aggtatttta tatggcaaat cttatagtct tggagatggg
     1681 cttgctatta aatttttaac acacgatggt agcaatgaaa ccattattgc aactgatgct
     1741 aataatggac atctttattt ctcaagcaat ctaggcagtg attgggaaat agctacaggg
     1801 ctagtagaga tagaacattc tttatttgaa gaatcgtttg gcatacacat tgtcgaggaa
     1861 gcagcctcaa atccaatggt gttgtacgct ggttatattc aatcaaagat taaaggacag
     1921 gaccctcatc aattagacga aattaagcag tcaccgggaa tgtataaatc tgttgatgga
     1981 ggaatgtctt ggtttccaat taataatggg ctacccgatg aagactacag ccgtaatgta
     2041 tcagatattg ctgttagcag tcagaatgaa gacgttgttt atatcaattt attatttgat
     2101 gggttgtact atactgtgga tggtggtgaa tcctggaaac aacttaaagg taagttgcca
     2161 gtgggtatgt catggcatga ttgtggacac acgtgtgtta atgaggatgg cgaaagagtt
     2221 gacatggaag atatggagca atccgaaatg attcaacgta aacacgcgat gagtatcgct
     2281 gttaacccac aaaacgatca agagatattt ctaggtacca atgttcatgg tgtttataag
     2341 tccatagata ggggagcaag ttgggaagaa acactttggc gtacagaggc gatcaataca
     2401 tcgaaacgcg accacgcaca cgcaattgat atttctatta accccgcgaa ccctaacacc
     2461 attgctgttg ccgactggaa tagtggcgtg ctactgagtc aggatggggg tgagtcttgg
     2521 caacgaacta accaaggatt agattcaggc gtggttcaag tgctaacatt ttcaccaaac
     2581 ggacaatacc tttttgctgg tactgaaggg catggcatat ttcggtataa attctttgat
     2641 taagagtttt cggttattaa gtccaattca ttcctgatgc ctaatattat tatatgctga
     2701 atcgtactta gaaatactac tttgaagaaa tattaatacc ggctctacca tccagtatca
     2761 gcccgtgtcg ggaatttggt cggcaaaatg tcggcagaag tgaaccggtt ggagtatggc
     2821 ggcaccttta tattggtatg tagtgcctca gactatttct tgcctatttg gataccgtga
     2881 gacttacagg gccagataac tattggatta gaactcgttc ggccaccagg gctgtaagtc
     2941 agtcttgaac atattgtctg caagagccaa cgagccctgc ctgttttcgt gggcgcgtcc
     3001 cgcgcgagca agtgcagtga aggtaattcc tcccagatag gcggaagaca ggtccgcagc
     3061 tgatatagag atatcggagg atttgctggt cagaacacaa ctggatcctt gagggcttcc
     3121 ttcaagctca tagaaatttt cattccaggg gcagaaccta tcatttatct cgaacaccag
     3181 acgtccttca cagtcataac tccgttccga tagggcatct ttgacagcaa ctaaacgaag
     3241 ccaaagatcg tccctcaggg actgatgtag tcgccgaggg tctgacagca tccagggtag
     3301 aggatcgtca ataggacgtt tgggcgcatc aatacgtctc attaaatcta ccccgaagca
     3361 atacctccag agcgctgtat gagatgttga ggtcgccgat atcatctcgt gaatcagtaa
     3421 gatatctttt tttatccggt agctcacata tccatctacc tgtccatttt cctcatagac
     3481 aacgtggaac agctcgctgg catcaccccg acggtactca gggtctgaca ggtatagctt
     3541 ccacagggat ttggtgccct taataaaccc tgaccgttta gcattggcgc gttcagccac
     3601 ttcgggtagc gtgtcaaggg cgtcaccact attgataaag ctgtacttac cctttgtcga
     3661 aacatcatta ataaacgctg tatggcgacg atcaatggag aaatctacct gcctggaacc
     3721 tatgccataa ccatatctgc cgtagattat tgactcagag gcccctagag cagacaaagt
     3781 ttccccacgc tcataaaagt cgcgcatgtg aaaatccatc attcggttga gaatgcctct
     3841 tcgccgatga gttggcaata ccgccacatt gtctaagagg gtagtgggaa gtctttctat
     3901 tcccacgttc attttgaggg gataggcgat gccaaaccct acgataatac cttcatcaaa
     3961 cgcagcgacc cctcgaccgt catttagggc ttcagtaata agaggcaatg cagctttgtc
     4021 gccatgtccc ccgttggcgc gttctaccgc aatatgactt tcaaaaattt cagctttctg
     4081 gatataccga atttctattg ccaaatatct gcttcctcca tctgagaatg ggagccagat
     4141 tcatgtttca tttgcaaaca ttcctgcaat ttttgaaata ataagattta acgcatagta
     4201 acattttgac taacacacgg tgtgattgga attcgcactg ttaggggtag ataaatgggg
     4261 tacgcacgaa ggggtgtcag gtagcattct cgctgatgcg aaccgtggat gggggagtgc
     4321 ctctatatag ggtagaaaga gcattttcga tttcaagaag ccttttgccg tctttgagat
     4381 cagacaggaa attggaaccg ctaaagataa taggtcataa acccagtctt tttctgttca
     4441 ctatgaccaa aggcaacatc aacattgcca gtacagaaac accttcacga gccgaggtgg
     4501 atgcacatga accaccagtt gagtcttctg ccatatcacc ctgcccttct ggctgtgttt
     4561 catcttcacc tgtggctctt ccaacaagac taaacctact tctgctgctc gatgatctgc
     4621 ttgcggttgt cttcttctcg tcatcctttg aaggtgaact cctgaaccca aagaagttgc
     4681 caaaagtcct cctcttccca tgacattcag acgctagaga acttaacctt gcgggaaggt
     4741 gtgattacga acggaatcga gaacaaaaac ccagtattct tactcaccac tgaacgaaca
     4801 ttggcattac tacgtgagtg tagccttctt tgcctgtagc ccgtaccacc ccagggcttg
     4861 aagcggaagt catctcaaaa gcaacctcgc cttcgccaag aacatccaag acttcagcca
     4921 aatatttact gttgaacgcg atcctggatt cttcatccac atcaccttca actccaccgt
     4981 ctatttcgcc ctggttatct ccaacttctt ctgctctgga ggatatggaa agtttccccc
     5041 catcatcatc attcataatt tggatcctga gtattccgct gccatcacga gcaaatattg
     5101 aagaagtctt tacagcacgt gaaaaatcag aattttgaac gatcacacga tttttatatt
     5161 ctgcaggtat aagagatctg aagtttggaa aagaacctgg cataagttga gagacaatct
     5221 ctacattgcc gatattaaat agagcatgag ttccggctgg tgtgactgtg aactcaacag
     5281 cagtatcatc tgtaccgata agtcggccaa cttcttgtag ggccctcgca ggtattataa
     5341 attccatggc ttcagaaact ggttctagta atttaccctc atatacagcc agtctaaatc
     5401 cgtcagccgc tgcaaacgtg aaatctgact gattcacctc aacctttatc ccagtcaata
     5461 ccggacgaga atcctcagtt gctgcagcaa aggccacatg agttaccgtg tccttaagaa
     5521 gttgaggatc aacttttata gttgcccctt catcaactgt agggattgga ggaaactcgt
     5581 cggggtcggt gccattgatg tttgcagaga acctctgaca gtccaacgaa acactaagtg
     5641 gttccacaac tgacttaatt tctattcgat cgtcagggag tgaatttacg aaatctgaaa
     5701 gtaatcgtgc tggtattgtt atttcacctt ctgtttcaac ctgggcacca attttagtag
     5761 taatagcgat gtccaaattg gtagcggtta aaaccagcat tccatcttcg gtggcaattt
     5821 ttacgttttg aaggaccggt aaattacttc tagtagcaac cgccctgcct actattgcca
     5881 aggctcgact aagattttgc tggagacaag atacttgcat attgagcact cctcaggaat
     5941 tgtgactcaa ggataggaac aagtatatta gccttaagaa gccctagtca acgagactac
     6001 tgtatttggt gtagtgaggc gtttaaacca ctacataaag tctactattc tttcgcagct
     6061 actacttcta attcaaggat aatttcgtca tcaacgctga taataaaagg gagcctgggt
     6121 ttggacagag aaaactgatc ccacgttatt attgttgaga tactgccagt tatgtcactt
     6181 tctgctatgt ctaatgtccc cttccaaata actgggtttg tgacatccga tatcgtcata
     6241 tcaccttcta tggtaacttc catttttccg aaatctggca gcggccaagt atgacctgat
     6301 actttggtta aagatatgga tatctcagaa cctattcctc cgttcctgcg aacccagtta
     6361 tctcttttgc tttcatcgct ttttaacgat tccacatcaa tcacgatagt ggaggcatca
     6421 gaactactga tattgccgga agggttaaaa tggacctttc catttatacc tgaggtttct
     6481 ccaacggcag ttattggggt aggtaggcga gcaaactgtt ctccgattct atatctggca
     6541 acagaaccag gttgtaaaga tataagtact gattctagtt tggcagttgt gggtgttgcg
     6601 gcaccttttg atgaggttgc caccgaagat aacggctttg tagggatcgg cttggtggtg
     6661 gggaccggat ttggaggaac cgtggcaatc ataggggttg ttctaatggg agaatctgga
     6721 gttgcggcag gtgccgtttt cttcacctca taaggttttt gatcaacact agcggcagca
     6781 ggttcaacag ttaactgttt cagtggtgcg gaggttactt ctggggtctt tgctttccta
     6841 ggggtttcat cgacttgatc tgaagagcat ccaagcaaac caatcgaaag aataggaatg
     6901 atacaggcag gaccaagcaa cttcactatg ttcatctcgc cacccttgcc acttaataac
     6961 tttccatgtg atttagctat tgaacaatga ttttacctat cataccaagg gattcatggg
     7021 gtatacagat taaatcaaaa gtcccagctt cagaaaatgt aaatgtgatt tcctcagttg
     7081 taccagcatc tacttcaaca tctataccca aagaatctac agtaaagcta tgaaactcat
     7141 tttcggaggt taaattcaag gtgactgttt cacctttttt taccgttata tcagtcgggt
     7201 taaatatata tatagcatta gcgtcattca tagcaacgtc caaagaataa tctgatgggg
     7261 cagctgctga cacacttgca gcagaagaag actgagaaga tgttgtagcg ggtgggggtg
     7321 cagtaaccgc tacactggag gattggttgc tagaagttga aactatcggt gccggtttca
     7381 cttcatcatg actccctttc ggtggtacgt tttcaccaca ggcataggtg ccaaatagca
     7441 aacctaacat cactacactt acgactaatc ctctgatcat gtctcctcct tgagaatgat
     7501 gcgttggtga taactttcac attctcagtt tagcatcgcg acttttgggg ggtcaacgac
     7561 tgaagatttt tgaatacata caaaattact ccctgtagta attgacatag tacacctgtt
     7621 ctgataattt gttcggaaca ttagtgctta tttatatgaa accttaaaaa aggaagtgac
     7681 attagtgaag cggccattat caaaacgaca gcagactatt ttgaactata tcaaagattt
     7741 tatgggcaac aatagctacc ctccaaccgt tcgtgatatt caaacgggat gtgaaataag
     7801 ctcaacgtca gttgttgact acaatttgca aatactccaa cgtgaaggtt atctacaaag
     7861 acgtagggaa gtctcgaggg gtatcgaatt attgggtgaa acagctacat cgacactgag
     7921 aaacctaatt tcaatacctg tggtaggaaa catcgctgca ggcgaacctt taccattggt
     7981 taatactgaa caatggaatg atactgattt tgacaatgtt gatttgcctc cttttttaac
     8041 caaggggaag gaaaatgttt ttgcattgcg agttaaggga gaatcgatgg tcgatgcatt
     8101 ggtcggagat ggagaccttg tactactgga atcgatcgaa caaccacaaa acggtgacat
     8161 ggttgctgcg tggttgaaag atcgggaaga ggcgacctta aaacattttt tcctagaagg
     8221 cgataaagtg aaattggttc ctgcaaattc ctatatgcaa cctattactg ttgcagccgc
     8281 caatgtatca gtgagagggc gagtagtagg agttgtccga acaatgtgac caatccgaat
     8341 attgcctaat ttgagcttac gaaaaggccg gtagtatata ccggtctttt tattagacgt
     8401 atacttttgt caaataggca agcttaggga actggggtgt aatagatcgt ggacaataat
     8461 ctaattgaca catccttgac tatacccatt gttggaatga cgtgtgcagc atgcgtttcc
     8521 catgtaggtg cagcccttga gggcataccc gaagtggacc gggctgttgt gaatttagct
     8581 actgaaaaag ctaccgtaca tatttcctcc gggcacatcc aaaaagagct ccttaaatcc
     8641 accgtacagg atgccggcta tcaattgggt tctgaatcta tcacattgtc agtcgaaggg
     8701 atgacgtgtg cagcatgcgt gtcacatatt gaatcttcat tgaagaaatt agaaggagtt
     8761 gaagaagccc atgtgaattt ggcaactgag acagccaaga ttcaatatat ccctggtctt
     8821 gaatccatct cttcaataag acttgctatt actaattcag gatattcctc atcctatatt
     8881 gagcaagaag aattggggta cggatccact aaccgggccc aaaagcgcat tgttcaacag
     8941 gcgatagtca gcctgttggg agccggattt atcatgctgg gaatggtctc cagtttggcc
     9001 tctcaactcc ctttccaaag cgaatacttg tccttcataa ttgcctcgat tgtgcagttt
     9061 tgggcaggca ggcaatttta taccagcgca tggaatgcaa caaaacaccg cacgacaaac
     9121 atgaacactt taatagcggt tggaacttca actgcctata tatacagcac atttgcaatc
     9181 ctaggtaaat tatttgggtt aagtgacgcg tttcccgaaa cctatttcag tacttcctgc
     9241 gcgataatag gaatggttct tttaggaaga tatttggaat ccaagtcgag gcggcgtgcg
     9301 acggatgcga tcaaatcatt actggatttg gcacctaaga ctgctaatat catgaaaggg
     9361 cccgacgtaa agacagtatc agtagatgat cttgtcatcg gggatatagt cattatcgag
     9421 ccaggagaaa aaatccctgc agatggatca gttgtcgaag gtttttcgga agtcgatgaa
     9481 tccacactca cgggggaaag tgttcccaag gcaaagaaaa tgggggatcc agttttcact
     9541 tccactatca atggaactgg aaatattaag gtgattactg aatcactttc tgatggcaca
     9601 gtgtactcaa aaattgtttc cctgatagag gatactcagg cctcgaaagc tccgattcag
     9661 agaattgcgg atcgtttggc aaccctacta gtgcctgcca tattgatcgt agcggcattg
     9721 acttttttga cctggatcgt acttggccct gatccaacgt atgcttatgc cactattgca
     9781 gcagtagcag tactggtgat tgcttgtcca tgtgctatgg gtctagccac ccctatagcc
     9841 ataatggtgg gaagtggaag gggtgctgaa aatggaatct tgtacagaga tgccctagca
     9901 attgagaccc ttcaaaaagt tgattccgtt gttttcgata aaacaggcac gttgacgaag
     9961 gggcaaccgc aggtcacggg ggtagattcc cactcaagct attctgagac tgaaatcctg
    10021 gaatacgcag gtgccgttga aaaaaattct gaacacatta tcgctaaggc aattgtggcg
    10081 gaatgtgagt ttaggaaaat tgtgtcagca gacgcctcag aattccggtc ttacccagga
    10141 ttaggtacat ctggagtttt aaatgagaaa ataattttag tgggaaataa aaattttttg
    10201 agagaggccg atattgaggt accagaggtc ttcaactcag ccgatcttgg gggggtacat
    10261 gtatccatta acggttcata ttctggatca atattttttt cagataccat caaagatgac
    10321 gcagcggaga cgatagctca tttgcatgag ctcaacttga atttggtatt acttagtggc
    10381 gatcgggaag aattggttga atcagtagct aatatagtaa atataagtag ttatgaatct
    10441 aattttctac ctcaagataa agcagcttat atttcaacta tgcaaaaaaa tggacaagtc
    10501 gtttgtatgg ttggagacgg ggtaaatgac gcccctgcac ttactcaggc ggatatagga
    10561 atagcgatgg gcggtgggac tgatatagct attgaatcag cacaaattac aattctagga
    10621 aacaggctat cgtctatagt ccaatcaatt cgcctaagca aatcatgtat aaccaccata
    10681 aaacaaaacc tgttttgggc t
//
LOCUS       DASCNS010000251         1380 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001883756, whole genome shotgun sequence.
ACCESSION   DASCNS010000251 DASCNS010000000
VERSION     DASCNS010000251.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001883756"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="QF838_09035"
     CDS             complement(<1..1012)
                     /locus_tag="QF838_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJO60862.1"
                     /translation="MDKALKGVKIIDMSRVQAGPSCAQIMGFLGADVIKLEDTAGGDN
                     TRVDMAHVKGVDSVYYTIFNGNKRAMTLNLKSPEGTEIMDKLVKWADIILENFSSGVM
                     DRLGFAYERLREINPGIIYASIKGYGDYGPYSSYKAFEYTAQATGGLMAANGLPDGPP
                     MGTAAGIGDSGTGLHMAIGILAALRQRDKDGEGQRVEVAMQDGVVNLMRIRLIRSLST
                     SEPNVRGVVLGKTNIPHTFACSPGGYDDYVMIHLRGYMWETVLAVMGKEDLIGDERYI
                     DEQARNKRPKEVVDIITSWTSTKSKYEVFHEMAAAGVWCGAVISPEEVITNEHLIARE
                     MVV"
BASE COUNT          363 a          345 c          248 g          424 t
ORIGIN      
        1 ctacaaccat ttctctcgct atcaaatgct cgtttgtaat aacctcttct ggacttatta
       61 cggccccgca ccatacccct gcggcggcca tctcatgaaa tacttcgtat ttagatttgg
      121 tggaagtcca ggaggtgatt atgtcaacaa cttcttttgg tctcttgttt cgagcctgct
      181 cgtctatata acgttcatcc cctatcaggt cctccttacc cataacggct agtaccgttt
      241 cccacatata tcctctcaag tgaatcatta catagtcatc atatccacca ggactacagg
      301 caaatgtatg cgggatatta gtttttccta agacaactcc tctcacgtta ggctcggaag
      361 tactaaggct tctgattaaa cggattctca ttaaattgac taccccatct tgcatagcca
      421 cctccacgcg ttgtccttct ccgtctttat ctctctgtct cagggcagca agaataccta
      481 tagccatgtg gaggccagta ccagagtcac cgataccagc cgcagttccc atcggtggac
      541 cgtcaggcaa accatttgcc gccattaatc ctcccgtggc ttgtgccgta tattcaaaag
      601 ccttgtagga gctgtatgga ccgtagtccc catatccttt gatactagcg tagattattc
      661 caggattaat ttcccttaat ctctcataag caaatcccag tctatccata acacctgaag
      721 aaaagttctc caaaataatg tcagcccatt tgacaagctt atccattatc tcggtacctt
      781 caggggattt gagatttaat gtcatggcac gcttattacc gttaaagatc gtgtaataaa
      841 cactgtcaac acctttaacg tgagccatgt caacgcgtgt gttatctcca ccagctgtgt
      901 cttcgagttt tatcacatcg gctccaagaa aacccataat ctgggcacat gacgggccgg
      961 cttgtactcg actcatatcg ataattttca cgccttttag tgctttatcc atcttaatct
     1021 cctaacctgc tggcgataat ttttccgttg atgcacttaa tatttttcag ttattttgga
     1081 tagggtgttg acatattgtc gaccataaaa tcattccttt ttaaaaaggt acgcagtcac
     1141 gaggtttcca aaaattgcct cctccgctta aacactgaaa ggtttgtttg tactctatat
     1201 catctaagca tttaaaaact gtttagctac ataaaatcct cactcaaaca gggtttagtt
     1261 caggaaacac gcttatggta atctgccaga tttagttaaa ttagctagta ggtcgtcgta
     1321 tgcattacgg ttgactggca aagagattct attaccggtg caaccagaca aaatgatggc
//
LOCUS       DASCNS010000252        12234 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001897386, whole genome shotgun sequence.
ACCESSION   DASCNS010000252 DASCNS010000000
VERSION     DASCNS010000252.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001897386"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(132..207)
                     /locus_tag="QF838_09040"
     tRNA            complement(132..207)
                     /locus_tag="QF838_09040"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Ala,seq:ggc)
     gene            450..1214
                     /locus_tag="QF838_09045"
     CDS             450..1214
                     /locus_tag="QF838_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60863.1"
                     /translation="MRFQGKVAIVTAAANGIGKATSRILAREGAQLVAVDINPEALRD
                     LSKEIEGEGGHIIILETNVLEPNQVNDLVDTVVERFHKIDVLVNAVGGSTIIPNSTAS
                     VDNLSLDDWDRIIQFNLRGTFLCTSVVTRQMKKQGNGKIVNISSDAAHSMGDPSSAYV
                     AAKAGIMAFTKKVAREAGPYGVTCNAIAPSVTLSERVGPRWEQRSEENKQQILEEIPL
                     RRVSQPEDQAKVIAFLASGDADYVTGVTIDTSGGRY"
     gene            complement(1276..1746)
                     /locus_tag="QF838_09050"
     CDS             complement(1276..1746)
                     /locus_tag="QF838_09050"
                     /inference="COORDINATES: protein motif:HMM:NF017470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HJO60864.1"
                     /translation="MARKNFTAYTFRWLVIFASLLAKSPIQPLRNILDYKGRATVREF
                     WLFFIFWVNLIPFILGYYIAGIFGVDLYLDVLVILPYVSLCVRRLHDVGWSGKWVILI
                     EGLWYAFMTISVLESIGLLQFLCLLLHIVMAIVIYGRKGREGYNQYGEQPYDYV"
     gene            complement(1878..2165)
                     /locus_tag="QF838_09055"
     CDS             complement(1878..2165)
                     /locus_tag="QF838_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60865.1"
                     /translation="MAGISKTVVSLQPEKMEEVTKFIDSQSHLVTELDGMIGFAVAVT
                     GKDEITIIGIYESSKNAEAASDTVQKVFSDMASLMASPPDRGVYSGVWFSS"
     gene            2291..2770
                     /locus_tag="QF838_09060"
     CDS             2291..2770
                     /locus_tag="QF838_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HJO60866.1"
                     /translation="MFTMNCPTCGKENESSAQFCGICGSQLSDQQTTSIEYGVDSSKP
                     MVGFGEAISLGFKNYINFSGRATRAEYWWWCLFLVVTGVGLSIIDSITGIGALQNIFN
                     LATFIPGLALGARRLHDIGKSGWWQLLWFAILVGWIILIVWYCKPENQLGNKYNTLV"
     gene            complement(2946..3101)
                     /locus_tag="QF838_09065"
     CDS             complement(2946..3101)
                     /locus_tag="QF838_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60867.1"
                     /translation="MERKPAIHKASVGAGKIWFGKVTCAPNQKGSPHHHGEAETAADI
                     MANLFPH"
     gene            3294..3578
                     /locus_tag="QF838_09070"
     CDS             3294..3578
                     /locus_tag="QF838_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60868.1"
                     /translation="MVRSWGESSYRNQSGIKMREELCIDCDKDNVTKIESNIKTPLIL
                     LMDNHLRCKNHASDFLDQREKMLKNSTNLPTSITEQRMLDEVRVMKAKFK"
     gene            3602..5890
                     /locus_tag="QF838_09075"
                     /pseudo
     CDS             3602..5890
                     /locus_tag="QF838_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            5916..6995
                     /locus_tag="QF838_09080"
     CDS             5916..6995
                     /locus_tag="QF838_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024771.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO60869.1"
                     /translation="MKISSIEYVVLDGLHPFVFIHTDEGITGIGECFRRQPLVTKTVV
                     EELLAPSILGKDPVDTESRFRDMAVAGQALEIGGAIWIGIAGLDIAMWDLKGKILNLP
                     IYELLGGKVRENVPVYASSMARNLTPLQEANRALSFVEKGYTGYKLHSAIPGRIDDSA
                     DQTIATVTEVRKAVGDDIDILVDVNGAYSVHHAIEIGKQLEELGVFHFEEPRPHYDLP
                     GLAKVANALDIPVASGEMIYSLYEYKELMMRGEIDIVQPDIVKTPGFTTLIKIANLAE
                     SLGVPITCHNTQPTISTVAHAHFVAATPGAPYSQEYNIESVSIRDEHPILSEPLQIAN
                     GLLEVPNGPGLGVSLDMKMVSKLKT"
     gene            7262..7660
                     /locus_tag="QF838_09085"
     CDS             7262..7660
                     /locus_tag="QF838_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJO60870.1"
                     /translation="MTNRHHIPEKSRQYSGLANLVKEVRLGKNLSQREFSRMLGMSNA
                     YVAHLEGGKIQPSVKTLRNISTTLGLPYNRLALLAKYIDPSIFDRPANADDSIRFKAI
                     ADLTDEEWQSVLDYIDYVRSKRNNSVDGSN"
     gene            complement(7657..8655)
                     /locus_tag="QF838_09090"
     CDS             complement(7657..8655)
                     /locus_tag="QF838_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013595.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO60871.1"
                     /translation="MTLPFELLRSGYPYYMTLGQRRVTSNPVDIGFGKENVYVLTRGG
                     LGTEVRVINWEDENLGTLGGASLFQWPAALLVDEDENLYVTDEANHKVVVMDKDGEHI
                     NTWGEQGSEPGQLNRPSGMSFDAEGNIVLSDAMNSRIQRFTRDGKHLQAFGEFGNGEG
                     ELNMPWGLTVDQYGDVLVADWKNDRVQRFSSEGEFKSAIGSHGIEKGEFRRPSSVAVD
                     GHGDIYVSDWQNDRVQLFNRDERYIQSFHGNASLSRSGKQYVLSNLVTLRLRAEGDFE
                     KTQRLNAPMTVKVDDQFRLFITDFGNHRIQIYQKDAVELSETEIAPKMRNPILFTT"
     gene            complement(8675..9817)
                     /locus_tag="QF838_09095"
     CDS             complement(8675..9817)
                     /locus_tag="QF838_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HJO60872.1"
                     /translation="MTTFTQKDPVFVVVQLSGGNDFMNTLVPYSNSAYYDNRKFLNIS
                     EEDVLPLDGTLGWHPEMAPFKELYDRGMVSVVQGIGYPDSNRSHFRGMDIWHTCAPDE
                     VSTIGWLGNVIEQLDPSGENPLTGVNFGVGLPRAMSKAGVPVTSVSNLDEYGLMSGIS
                     AQQQRVQALQIFKDMYGQAVGSGPVMDYLSRTGMDVLSGADLIKVAPQQYESTVEYAD
                     NPIAKSLRDVARVHTANLGTRIFYTQQGGYDTHANQLPTQPGLFRDMSRAVNDFFADL
                     EEHDADENVVMLIFSEFGRRINDNGSGTDHGSGGGAYLVGKPMAGGLYSEYPSLERDK
                     WEKGEDLAHTIDFRGIYGTVIEQWLGLEAAPIVYGNFEQINPFRAN"
     gene            complement(9850..11253)
                     /locus_tag="QF838_09100"
     CDS             complement(9850..11253)
                     /locus_tag="QF838_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HJO60873.1"
                     /translation="MTNNDLALKAHLLRRAGFGASRFELEQISDKSYEEIVEDLVHPE
                     RFEEIDEDYLKRYNPELSYHDGHPIHAGRWIWRMINTKRPLEEKMTLFWHHVFATAHY
                     KGDHTPSTIGQIETFRENGLTNIKQILLDLAKDPHMNYWLDNCENHSDQPNENWGREL
                     LELFSMGVGNYSEDDVKQAARAFTGWTFEQPLPLYPYGHYESKFVFNESDHDNSEKTF
                     LGRKGNLNGEDIINIICEERATALFICRHLYNFFVEDEAQVPAWSIEPPRNPEAVDTM
                     IDILMNNDGEIRPVLSYMFNSDFFKNSFYKKVKNPSELVAGTLKLSGRYSIVPAEGEN
                     VGTLYNTVAVMGQALMNPPTVEGWHTGHEWIDGGTLNERVNFAVNQFNDVSSPGFKDI
                     LERLGSVVQSGDLVDRCLDLIGPIEVGEETRIALSNYAEEVGDVNLSTDEDKVENAQK
                     IARMIQLIVSTREYQFA"
     gene            complement(11348..>12234)
                     /locus_tag="QF838_09105"
     CDS             complement(11348..>12234)
                     /locus_tag="QF838_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HJO60874.1"
                     /translation="PPGLVVAEMSRILRASPKAVREIAQDAMREIFSQMKKGNLGAHD
                     TFKKGDWGDLTGQTIPYEFGQSFEINLQKSLFNTVVREGSSIPLKIQSRDLEVNEHEH
                     LTQSATVLMLDQSRSMGMYGTFTAAKKVALALYWLVQTKFPRDKLFVVGFSDYGMTID
                     GKDLPESTWNHWVAGTNMHHGLILSRQLLARQNVANKQILMVTDGEPTAHMEGGQAYF
                     SYPPTTRTLEQTLKEVKRCTREGIVINTFMLEMNYFLMEFMDEMAKINKGRSFYTKPD
                     QLGRYMLVDYLEGRKRKI"
BASE COUNT         3579 a         2733 c         2454 g         3468 t
ORIGIN      
        1 tcataatgcc gaataataca ccttttacaa taactcgcac gtaattccac attctcatgt
       61 agttggtatg atgtgccaat gaaagtggct gcttattgca tattattact atcgttagtg
      121 tttacttcct gtggtggagc tggggagact cgaactccct acctcttcaa tgccattgaa
      181 gcgctctccc agatgagcta cagccccacg tgatggtgaa tgattattat acaggttttt
      241 cgttcctgta taagctgtat tgtgggtgtt tggcgctgaa gggataggga atgcgagact
      301 ctctctattt tgagaagtgg ttgcagataa ggcctcctat ctagctctaa tctcctcatt
      361 ccaagggcgc tttccattaa ttatctggct ttttcttaga acatctccta aactgccggc
      421 aaagttaggt tcaactaagg agggaaggaa tgaggttcca aggcaaggta gctatagtta
      481 cagcggcggc taatggcatt gggaaagcta cctcgagaat ccttgcacgg gaaggagctc
      541 agttagtggc tgtggacatt aaccctgagg ctctcagaga cttgagcaaa gaaatagagg
      601 gtgaaggggg gcatattatc atccttgaaa ccaacgtatt ggaaccgaat caagtcaatg
      661 atttagtgga caccgtagtc gagaggtttc acaaaattga tgttctagta aacgcggtcg
      721 gaggcagcac tattattcca aatagtacag cctcagttga taacctttct ctagatgact
      781 gggataggat catccagttt aacttaaggg gtactttttt atgcactagc gtcgtcacca
      841 gacagatgaa aaagcaaggt aatggaaaaa tagtcaacat atcttctgac gccgcccaca
      901 gtatgggcga ccctagtagc gcttatgtcg cggccaaggc aggcatcatg gcttttacaa
      961 aaaaggttgc tagagaggcc ggaccatacg gtgtgacctg caatgcaatc gcccctagtg
     1021 tgacattaag tgaaagagta ggccccaggt gggaacaaag gtcagaggaa aacaaacaac
     1081 agattcttga ggaaattccc ctaaggcgtg tatctcaacc tgaagaccag gctaaggtca
     1141 ttgcttttct agcatctgga gatgctgatt acgtcacagg ggtaacgatt gatactagtg
     1201 gtggtcgata ctaagtaaga aaattcgtgt atatacattc ctgaatttgc tattgatcta
     1261 acatttcagg gaaatttaaa cataatcgta gggctgttca ccatattggt tgtaaccctc
     1321 tcggccttta cggccataga tgacaatcgc cataactatg tgtagtaaca aacagagaaa
     1381 ctgcagtaac cctattgatt ccaataccga tatcgtcata aaggcgtacc acaacccctc
     1441 aattaaaatc acccatttcc cactccagcc aacatcatga agccgtctca cgcatagcga
     1501 tacgtaaggc aatatcacta gcacatccaa atacaagtca acaccgaaaa ttccagctat
     1561 gtagtagcct aaaataaaag gtatgagatt aacccaaaaa ataaaaaaca gccagaattc
     1621 cctgactgtt gcccgtcctt tgtagtcgag gatgtttcgt aaaggctgta tagggctttt
     1681 agccaacaac gaagcgaaaa taaccagcca tctaaatgta tatgctgtga agttttttct
     1741 agccatcttg tcatcctagc ccaaatatta gtaatgaact aggagtattc aaagaatgtt
     1801 tatatccctc aaataatatt gtgggagggc gcaagagtta gtaaatgaaa ttattacatc
     1861 atttcccatc atgtcgttta gcttgaaaac caaaccccag aataaacacc tctgtcaggt
     1921 ggagaggcca ttaaagacgc catatcagaa aagactttct ggaccgtatc agatgcggcc
     1981 tcagcatttt tactactctc atagatgcct attattgtta tctcatcttt ccctgtcaca
     2041 gcaaccgcga atccaatcat accgtctaat tcagtcacta aatgggattg tgaatcgata
     2101 aatttagtaa cctcttccat tttttctggt tgtaaactaa caacagtctt agatattcca
     2161 gccattctca caactccttt tatcatttaa atattaattt atataaaaca atgatttcca
     2221 ctcctaatta tgctgatctt attgctatta tcaaatgatt acagggatgg aagggtgctc
     2281 atctgctagt atgtttacta tgaattgccc tacttgtgga aaagaaaatg agtcctcagc
     2341 ccaattttgt ggaatatgtg gctctcaact atctgatcaa cagaccacaa gcatcgagta
     2401 tggtgtcgat tcatcaaagc ccatggttgg cttcggggag gccatcagct taggatttaa
     2461 aaattacatc aacttcagtg gccgagcaac gagggcagaa tattggtggt ggtgtttatt
     2521 tctagtagtg acgggagtgg gtctgagtat tattgattcc ataaccggga ttggtgcgtt
     2581 acaaaatata tttaacttag ccacatttat acctggttta gcactgggag caagaaggct
     2641 gcacgacata ggaaaatctg gatggtggca gctattatgg tttgcgattt tagttggctg
     2701 gattattttg attgtttggt actgtaagcc tgaaaatcag cttggcaata aatacaatac
     2761 tctcgtatag ttcaaatctg agtggatctg taggtccatt aatatgatgg ataaaaccac
     2821 cggaacccca cccatattac ttcaaagtat ggatcctatt aacggttctt atttttttta
     2881 ctactttaag aattaactaa aatactgtca atgtgtgttc tattcaaggt aactcactta
     2941 gaatttcagt gtggaaatag gttagccatt atgtcagcag cagtttccgc ttcaccatgg
     3001 tgatgaggtg accctttttg attgggagcg caggttactt taccaaacca tattttaccc
     3061 gcgcccactg aagctttatg aatggctggt ttgcgttcca ttccgagtgt ctgggcggtt
     3121 cctgtcgcag gctcagtacc atgtactaca tagcgatttg acctttgggg gttgttgcca
     3181 tattaatatc tcctcaaaga ttagcaatac tcaacatggg taatacatgt aatacgcatc
     3241 agatagaaat atttacccta gggcgatgtg tccatcatga ggtttaattt gatatggtac
     3301 gtagctgggg agaatcttct tatagaaatc aaagtggaat taaaatgaga gaagaattat
     3361 gtatagactg tgacaaagat aacgtcacaa aaatagagag caatattaaa actccgttaa
     3421 tactcctcat ggacaaccat ttgagatgca agaatcatgc gtcggatttt cttgatcagc
     3481 gtgaaaaaat gcttaaaaat tccacgaacc tacccacctc tatcacagag caaaggatgc
     3541 ttgatgaagt tagagtaatg aaagctaaat ttaagtaatt acataggaga agatccaggc
     3601 agtgacaggc acttttcccg acataagaat cttagatttc agccagggca ttcctggaat
     3661 gtatacgtct cttttgatgg gtgacatggg agcagaggta attaaaatag agccccttgg
     3721 cggggaccct ttacgctctg atccaaatta cagattctac aatcgaggga aaaagagtat
     3781 ctgcttggat ctttcctctg acaaggaaat ggaaaatctc cactctctaa ttaaatcttc
     3841 tgatgttatt gttactactt ggctactgtc tgaagccaaa tccgctttcc tagattacga
     3901 gactctttcc aaaatcaatc cgtcaatttt atattgttcc ataccgcctt acggagatgt
     3961 cggccccatg gctgatattc caggagatga aggtactatc ggtacatata caggcatcca
     4021 tgaagggcaa ggtggagaga ctggaactcc actatatgtc aaactcccat ttcccaccta
     4081 cggaacagcc ttcacagcgt ctcttgcagt gtcagcagcc ttatttgaaa gagaaaccag
     4141 cggattaggg cagaaaatcg aagttcctct atatgctggg tcaacggcga tgcaagctac
     4201 cggtttcatc accggtgaaa aagtgattac tccagctcgg cgaaggggac ccgggccatc
     4261 aactggatta ccggtatatc gtttatacaa atgttcagat gactggattt ttgtggcatg
     4321 cgggaataat attttttgga ataagttgtg catggccctg gaatgctttg atttgctgga
     4381 ggatagtcga tttttggaag caccctggaa catcccttcg gagcactggt cggctttagc
     4441 agagatattg gaaccgattt ttgcttccaa ttccagagat cattggttaa atatcctcag
     4501 agaaaacgat gtgccatgcg ccccagccga gactcgaaaa tggttttccc agcacccaca
     4561 agtcatccac aacgaaatgc tacaaaaaat agaagaccct gaattaggat taacaacaca
     4621 ggtttcccct cctttgaaat tctctatttc aaagtccccc aaacctgaac cagcaaggta
     4681 tccaggagaa gataattcct tgttcgctac ctgcatccca aaggagccaa ttccgctcgg
     4741 aaaaatgaat cagcaccctt tagagggaat aaaggttgtt gatcttactg gttatatagc
     4801 cggagcctat ggcacgactc tattggccga tcttggagca gatgtattga aaatagaatc
     4861 gttcgctgga gatggattca gacaaaatgg agccgcattt caagggtgga atcaaggtaa
     4921 aagaggaatg attctaaacc taaaagacac gaaaggatta gatatatttc atcagttaat
     4981 cagggacgct gatgttgttg ccgaaaacta tagagggggc atagcagcaa atttgggtgt
     5041 tgattacaag agccttcgta aagtgaatcc taagattatc tactcaacag taaatggtta
     5101 tggtctcacc ggcccattca gtgaatatcc tgccttcgat cctcttattc aagcccaagg
     5161 aggtgcaatg aaagatcaag gcggctccgg ggatcctgta tttttaagaa ttgccgcctc
     5221 tgactactca taagcgattt tgtcagcata tggaatcgtc tcggctttgt attatcggtc
     5281 ccgccataac aaaggccagc acgttgaaat atctttagtg aacgccgcct tcgcttatca
     5341 ggcagcagag tatttcaatt atccagataa gccagcaata cccaggctag cctccgtagg
     5401 tcaatccgtc gactacaggc tttataaagc tgaggatggg tggttcttcc tttcctgtaa
     5461 ggaagatatc gaatggaaca agatgtgcca tgcggtagga atgtttgatt tagctatcca
     5521 caacaaagaa tcccgagctg agaatcaaga cgttcttaat cagaaacttg aatcattatt
     5581 tataaattct ccagtatcag tatggataga tattttccgc aaatcagggg tgaaatgcgc
     5641 tgtaaatcag agccttcgag taattcatga tgatccccaa gcacattcac tcggcctcat
     5701 tactgaaaat gattcctata atttagggcg tataaaactg caagggctcc cctttaggtt
     5761 ttcacgaact ccgggaaaac tagatttggc ggccccagac catggagaac atacagatca
     5821 agtgctccga gaactcggat tcttgaaaga gaatatagaa actctcagat caaagaaaat
     5881 agtaggataa aaataattaa attagggtca cacaaatgaa aattagctca attgagtatg
     5941 ttgtattgga cggacttcac ccttttgttt tcattcatac agacgaaggt atcactggca
     6001 ttggggaatg tttcagacga caaccattag taaccaagac agtagtggaa gagcttcttg
     6061 cgccttctat cctaggtaaa gatccggtcg acactgaatc cagatttagg gacatggcag
     6121 tggcaggtca ggccttggaa ataggtggag ccatttggat cggaattgcc ggtttggata
     6181 ttgcaatgtg ggacctgaaa ggaaaaattc tcaacttacc catttacgaa ctacttggag
     6241 gaaaggtgag ggaaaatgta cctgtgtacg ctagttcaat ggctcgaaac cttacaccac
     6301 ttcaagaagc aaatagggcc ctatcctttg tggagaaagg ttatacaggc tataaattac
     6361 acagtgcgat tcccggaaga attgatgact cggcggatca gacaatagcc actgtaactg
     6421 aggtaagaaa ggcagtaggt gacgatattg acatattagt agatgtgaat ggggcctact
     6481 ccgtgcatca tgcaatcgaa ataggtaagc aacttgagga attgggagta ttccactttg
     6541 aagaacctcg acctcattat gatctccctg gtcttgctaa ggtggcaaac gcacttgata
     6601 ttcccgttgc ctcgggggaa atgatataca gcctgtatga atacaaagaa ctaatgatga
     6661 ggggagaaat agacatcgtc caaccagata ttgtcaaaac acctggcttc actaccttaa
     6721 ttaaaatagc taatttagca gaatctcttg gagttcctat aacctgccat aatactcaac
     6781 caaccatcag tactgttgcc cacgcccatt ttgtggcagc aaccccaggc gctccttact
     6841 ctcaggaata taacattgaa tcagtttcaa ttagggacga acaccctata ttgtctgaac
     6901 ccttacaaat agcgaacggg ttactggaag ttcccaacgg accaggatta ggagtctctc
     6961 tagatatgaa aatggtttct aaattgaaga cctaatcctc aagacaaaat gaaaatcccc
     7021 cagccggtca aaagaaccaa aactagggga taccctctgc cgctttgata atctggagga
     7081 gcggctatta aaatgtgatt acactatagc ttttttgttc tatgttgtca aatcttttcc
     7141 gaacattata tctaaataat tttgtttttt aattaatact attaattaaa taccatttat
     7201 ctgaacaata tcccatgaaa tctattacca taggaatgat acaagcacag gaatgcttaa
     7261 tatgactaat agacaccata taccagaaaa aagtaggcag tattctgggc tagccaattt
     7321 ggtgaaagaa gtacggttgg ggaaaaacct gtcccaaagg gaattctcca ggatgttagg
     7381 aatgagtaat gcatacgtcg cacatttgga gggaggtaag attcaacctt ccgtgaaaac
     7441 gctgcgaaac atttccacga ctctaggact cccctataac aggctggcac tgctcgccaa
     7501 atacatcgac ccgagcatat ttgaccggcc agctaatgct gacgattcaa tacgatttaa
     7561 agctatagca gacctaacag atgaagaatg gcagtccgtc ttagactata ttgattacgt
     7621 tcgcagcaaa aggaacaatt cagtcgatgg cagtaattag gtagtaaata aaatagggtt
     7681 cctcatttta ggagctattt ccgtttcaga taactcaacc gcgtctttct ggtaaatttg
     7741 aatcctgtga tttccaaaat ccgttatgaa caatctgaat tgatcatcca ctttcaccgt
     7801 catgggggcg ttcaatctct gggtcttctc aaaatcaccc tcggcacgca gccttagggt
     7861 aaccaggtta ctcaaaacat attgtttccc agacctagaa agtgaagcat ttccatggaa
     7921 actttgtata tacctctcgt ccctattgaa aagctgtact cggtcatttt gccaatcaga
     7981 aacgtatata tccccatgtc catcgactgc taccgaagat ggcctacgaa actccccttt
     8041 ctcaatacca tgggagccga tagcagattt aaattctcct tctgaagaga acctctgaac
     8101 tctatcattt ttccagtcgg caacaaggac atctccgtac tggtctaccg ttaaacccca
     8161 aggcatattt aattcccctt ccccattacc aaattcaccg aatgcttgta aatgctttcc
     8221 gtctcttgta aatctttgaa tccgactatt cattgcatcc gaaagtacaa tgttgccttc
     8281 tgcgtcaaaa gacattccag atgggcgatt tagttgtcct ggttcagaac cttgttctcc
     8341 ccaagtattg atatgctctc catccttgtc catcacgaca actttatggt ttgcttcgtc
     8401 agtaacatat agattttcat cctcatcaac tagcagagct gctggccatt ggaacaggct
     8461 agcgcctccc agggtaccca gattctcatc ttcccagttg ataacccgaa cctcggttcc
     8521 caatcctccc cgagttagaa catatacatt ctccttgccg aacccaatat ccaccggatt
     8581 agatgtcacc cgcctctgac ccaaagtcat atagtatgga tatcctgatc tcaataattc
     8641 aaatggtaat gtcattgcca ccacctccta tattctagtt tgctctaaaa ggattaatct
     8701 gttcaaaatt cccatacact atcggcgcag cttcaagccc cagccactgc tcaatgactg
     8761 ttccatatat gcctcgaaaa tcaattgtat gagccaaatc ttctcccttt tcccatttat
     8821 cacgctctaa agaggggtat tcggaatata aacctcctgc cattggtttc cctaccaaat
     8881 atgcacctcc tccggaccca tgatccgtgc ctgaaccatt gtcattaatt cttctcccaa
     8941 actcagagaa aataagcata acgacatttt cgtcagcgtc gtgttcttcc aaatcagcaa
     9001 agaaatcatt aacggccctg gacatatctc taaataggcc aggctgagta ggcagttgat
     9061 tggcatgcgt atcatatccg ccctgctgtg tgtagaaaat gcgggttccc aaattggctg
     9121 tatgaacgcg ggccacatct ctcagggact tcgctatcgg attatctgca tattccacag
     9181 ttgactcata ctgttgtggc gctaccttaa ttaaatcagc cccagacagc acatccatcc
     9241 cagtacgaga caaatagtcc ataactggtc cggaccccac agcttgaccg tacatatctt
     9301 taaaaatttg gagcgcctga actcgctgtt gttgggcaga aatcccgctc attaatccat
     9361 attcgtctaa gttggataca ctcgtgaccg gaactccagc ttttgacatt gcccttggca
     9421 gccccacacc aaaattaact cctgtaaggg gattttcgcc gctagggtct agctgctcaa
     9481 tcacattacc aagccatcca atcgtcgaaa cctcatctgg tgcacaggta tgccaaatgt
     9541 ccatacctct gaaatgactg cgattagaat ctgggtagcc aattccctgg acaacagaaa
     9601 ccatacctct atcgtacagc tccttgaaag gagccatctc aggatgccaa cctaaagttc
     9661 catccaacgg caaaacatct tcttcagata tattaagaaa tttcctgtta tcgtaatagg
     9721 cggaattaga atatggcacc aatgtgttca tgaaatcatt gccaccggac agttgtacca
     9781 ctacgaaaac aggatctttt tgcgtaaaag ttgtcattat tagttacctt tatctcaata
     9841 gatttcttac tacgcgaatt ggtattctct ggttgagacg attaattgaa tcattcgtgc
     9901 tattttctga gcattctcga ctttatcttc atccgtgctc aagttaacgt cccctacctc
     9961 ttccgcataa ttactaagtg ctatacgagt ttcctcacca acttcaatcg ggcctattaa
    10021 atccaaacat ctatcaacaa gatcgccaga ttgaactacc gaccccaatc tttccaatat
    10081 atctttgaaa cctgggctag aaacatcatt aaactgattg acggcgaaat taaccctttc
    10141 gttaagggtt ccaccatcta tccattcatg tcctgtgtgc cacccttcaa cagttggagg
    10201 attcattagg gcttggccca tcactgcaac tgtattgtag agagtcccaa cattttctcc
    10261 ctctgcaggt acaatcgaat atctaccgga caatttgagt gtaccggcta ctaattccga
    10321 tgggttttta actttcttat aaaaggaatt cttgaaaaag tctgaattaa acatgtaact
    10381 tagaacaggc ctaatttcac catcattatt catgagaata tcgatcatgg tatcaacagc
    10441 ctctggattt cttgggggtt ctatactcca agcagggact tgggcctcat cctcgacaaa
    10501 aaagttatac aagtgtctgc atatgaataa agctgttgcc ctctcctcac atatgatatt
    10561 gattatatct tcaccgttga gatttccttt cctaccaagg aatgtttttt cgctgttatc
    10621 gtggtcggat tcgttaaaca caaatttaga ttcgtaatgt ccgtaaggat agagaggtaa
    10681 aggttgttcg aatgtccacc cagtaaaagc cctagccgct tgtttaacat cgtcttcgct
    10741 ataattccct acacccatcg aaaacagttc caatagttct cgaccccagt tttcattggg
    10801 ctgatcagag tggttctcac aattatcaag ccaataattc atatgcggat ctttagccag
    10861 gtcaagaaga atttgtttta tgttcgttaa cccattctct ctaaacgttt caatctgtcc
    10921 tatggtagaa ggagtgtggt cgcctttata atgcgcagtg gcaaaaacat gatgccaaaa
    10981 aagcgtcatt ttttcctcta aaggacgttt ggtattaatc attctccata tccaacgacc
    11041 agcgtgaatt ggatggccat catgatatga gagttccggg ttgtatcttt ttaaataatc
    11101 ttcatcaatt tcttcaaacc gttcagggtg aactaaatct tctacgattt cttcatagga
    11161 tttatcggat atctgttcga gttcaaacct ggatgcacca aatcctgctc gcctaagcag
    11221 gtgtgcttta agtgccaaat cattattagt catctcccct cctatcaagt attcctatcc
    11281 atcaaccttt catcgatagt tgctatatat cgctcattct agccaaatcg caattaatca
    11341 tcaacggtca gatcttcctt ttcctaccct ccaaataatc aactaacatg tagcgcccaa
    11401 gctgatcggg tttcgtataa aaagaccgtc ctttattgat cttggccatc tcatccataa
    11461 attccatgag gaagtagttc atctcaagca taaatgtatt tatgacgatc ccttcccttg
    11521 tacatctttt cacctctttc aaagtctgct ccaatgtccg agtagttgga gggtaagaaa
    11581 aataggcttg tccaccttcc atatgggctg tgggttctcc atcggtaacc atcaaaatct
    11641 gcttattcgc cacattctgc cgagccaaga gttgcctgga caaaatcaac ccatgatgca
    11701 tattggtacc agccacccaa tgattccaag tcgattcggg taaatcttta ccatcaatcg
    11761 tcatcccata atctgagaat ccgaccacaa acagtttatc tctagggaac ttagtctgta
    11821 ccaaccagta caaagcaaga gccacctttt tcgctgccgt aaaagtcccg tacatgccca
    11881 ttgatcggct ttgatcaagc attaacactg ttgccgattg ggtgaggtgt tcatgctcat
    11941 taacttctaa gtcccgagac tggattttaa gtggtattga tgacccttct ctaactacag
    12001 tattaaaaag agatttttga agatttattt caaaggattg accaaattca tatggtattg
    12061 tttgacctgt gagatctccc caatcgcctt ttttgaaagt gtcgtgggca cccaaattgc
    12121 cctttttcat ctgcgaaaat atttctcgca ttgcatcttg tgcaatctct ctgactgcct
    12181 tcggagaagc cctgaggatg cggctcattt cggccaccac gaggccaggt ggct
//
LOCUS       DASCNS010000253         1753 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001907869, whole genome shotgun sequence.
ACCESSION   DASCNS010000253 DASCNS010000000
VERSION     DASCNS010000253.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001907869"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="QF838_09110"
     CDS             complement(<1..882)
                     /locus_tag="QF838_09110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HJO60875.1"
                     /translation="MKVSTSFQEYSVDGIVGAARKSEERGYDIFSSSETGHNPYLPLV
                     LAAEHTEEIELQTSIALSFSRSPMDTAYIAWDIQNLSKGRFTLGLGSQVRGHIVRRFS
                     MPWSAPAPRMREYVMALKHIWDCWQNGTKLNFAGDYYSFNLMSPFFNPGPIEHPDIKV
                     SIAGVNPNMLRVAGELCDGVILHSFNTPTYTRDVVLPNLQKGAERLGRSVEDISISGG
                     GFIVAGENEEELEKKKKETKSRISFYASTRSYGDVMRAQGWDDTHEKLYRMSIDGKWN
                     EMGDEITDEMLDSFAVVG"
     gene            1055..1675
                     /locus_tag="QF838_09115"
     CDS             1055..1675
                     /locus_tag="QF838_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013077.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HJO60876.1"
                     /translation="MQNHLDLLEALYSQRAIRYFKDQDIPNEIIYRLIEIATKAPSGG
                     NSQPWRFLIIKNQDAKNKIGELYLQSWNQVYGSGSNGERNLEQKVMTSASYLAENIAK
                     APVWVMACLNHDGSDSHMGRGASIYPAVQNFLLGCMEFGLGSVITTLHKRYEKEVKEL
                     LNIPDNTETAALLPLGYLDEKVNYGPTKREPVDQITYQDSWGNPFK"
BASE COUNT          540 a          381 c          347 g          485 t
ORIGIN      
        1 acctactact gcaaaactat ctaacatttc atcggttatt tcatcaccca tttcgttcca
       61 ttttccatca atggacatcc ggtatagctt ttcatgcgta tcatcccagc cttgagccct
      121 cataacatcg ccgtaactac gggttgatgc ataaaaggag atacggctct tcgtttcttt
      181 tttctttttt tctagctcct cttcattttc acctgcaaca ataaaccccc ccccagaaat
      241 tgatatgtcc tctactgacc tgccaagtct ttccgcacct ttctgtaggt taggtagaac
      301 cacatctctt gtatacgttg gagtgttgaa tgaatgaaga atcacaccat cacaaagttc
      361 acccgccact ctaagcatat tagggttgac accagcgatt gaaactttaa tgtctgggtg
      421 ttctatcggt cctggattga aaaatggtga cattagatta aaggaataat agtctcctgc
      481 gaagtttaat ttagttccgt tctgccaaca atcccaaatg tgtttcaaag ccatgacata
      541 ctctctcatc cttggagcgg gagctgacca tggcatggaa aatcttctga ctatgtgccc
      601 tctaacttgg cttccaagtc ctaaagtgaa cctacctttc gaaaggtttt gtatatccca
      661 cgcaatgtat gcggtatcca tagggcttcg agaaaaagat agagcaatag aagtttgcag
      721 ctcaatctct tccgtgtgct cggccgctaa taccaaaggc aaatatggat tatgtccggt
      781 ttcactggaa gaaaaaatgt cgtaacctct ctcctctgat ttcctagcgg caccaacgat
      841 gccgtcaaca gagtattctt gaaagctggt cgaaaccttc atagtctttt atcccccgtg
      901 cattaatttc acaacaggtg gcattttaca cagatgctaa ggcccttaac aataatttcg
      961 gttcttccca ggccatttcc ttattaatag ctggtttttg tttgggtggc accatatata
     1021 atgcgatgca gcaacaaaac atattggggg aaaaatgcaa aaccatttag accttctaga
     1081 agccctttac agtcagcgag ccataagata tttcaaagac caagatatcc caaacgaaat
     1141 aatctataga ttgatagaaa tcgccactaa agcacccagt gggggcaata gccaaccatg
     1201 gagatttttg ataattaaga atcaagatgc gaaaaacaaa attggagaac tatatctgca
     1261 atcatggaat caggtttatg gaagcggctc gaatggagaa aggaatctag aacaaaaagt
     1321 tatgacttct gcctcatact tggctgaaaa tattgctaaa gctccagttt gggtaatggc
     1381 ctgcctgaac catgatggaa gcgattccca catggggagg ggggcgtcaa tctatcctgc
     1441 tgtgcaaaat tttttactgg gttgcatgga atttggacta ggaagcgtca taactacatt
     1501 acataaaagg tacgaaaaag aagtgaagga actgctaaac atcccagata atactgaaac
     1561 tgcagctttg ttgcctctgg gttatttgga tgaaaaagta aactacggtc ccaccaaaag
     1621 ggagcccgta gaccaaatta cctatcaaga tagttgggga aatcctttca aatagctaat
     1681 caaaaatcga agaggagata ggccatggag tacgaattca taaattacga aaaaaaggac
     1741 aagatagtct ata
//
LOCUS       DASCNS010000254        10336 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001914168, whole genome shotgun sequence.
ACCESSION   DASCNS010000254 DASCNS010000000
VERSION     DASCNS010000254.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10336)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10336)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10336
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001914168"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            50..1360
                     /locus_tag="QF838_09120"
     CDS             50..1360
                     /locus_tag="QF838_09120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60877.1"
                     /translation="MELLKRAGSWTPKTTVYYGWIVIAVGAMGTYAASGSAQVTLAGI
                     QTLMFEDTGWDRSTIALGITIGTWVAGLLTPVFGKIADTHGPRLVMPLTSVIVGVCFY
                     WIGGMSAIWHFYAAYIIARGLGNPVLIGVMPRTVAVNFFERKRNLVLGIVSMARPCFG
                     AINVQLITLISTWSSWRTAYKLLGMYSILLALPLIIFVRKDPESIGLFPDGVKKNLSS
                     LESAENPEKDSRNDGEIWSAGEAAKTFALWAVISAEFLIILTSGTIGFQLVPYLHESG
                     LSISMAALAWTLSTLLNAFSNPIWGFLSDIYSPRKLILSALPMSLGVTSIFLLIEGGY
                     PGFACVVIWGAATGGLNVLGGMIIANYYGRHSFGSISGIMGPFQIIGLGLGPIIGAIL
                     YDKTGGYEQLFIFAIIAYLFATILFFFARRPMLKLSKRTQKTLI"
     gene            1432..2274
                     /locus_tag="QF838_09125"
     CDS             1432..2274
                     /locus_tag="QF838_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60878.1"
                     /translation="MNNNSEISPNEAPSFQKARIAFAVSDLSVGTHRLAFGILEPGIG
                     AVKNEEIKLEIFFFQNDDRPILKETLSAKFQKWPIGDKGVYTSNVTFDVPGKWGVGVS
                     FNSTDGIVKKTSSVIQVTEKSQAPSIGDKAFPSNNSTINISRNLSYITTDSAPYKPFY
                     EYSIAESLNEGKAILIFFGSPAFCTTGTCGPQIDIVKSLHSDFSEYLIFIHVEIYENP
                     TELEGDISNAKIVQAVKDWNLPSEPWIFLVDQEGVIKSRFEGLATYQEIEIALIENNF
                     LISK"
     gene            2349..5882
                     /gene="mfd"
                     /locus_tag="QF838_09130"
     CDS             2349..5882
                     /gene="mfd"
                     /locus_tag="QF838_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HJO60879.1"
                     /translation="MDLIKITELLKSDPNYEGLVSEIIKGKPETRVQIIDEAVPFFTA
                     SLWKDLQIPILLICPSPEHSRRLHEYLSAWMDGESKPLRFGESEILPYERLTEDLSTS
                     RERLITLDSLVKGNSEPLVIVTSASALAQKTISIKSFKKNSFSYVQGDKVNLEDMANS
                     WQQMGYSFESNVSDPGTVSRRGGIIDVYPIGEDTPYRIELWGDEIDSIRTFDPDSQRS
                     INIVDRFSIGPAKETLISSTSADEFDRLLSHVDLSRCSPDEITRFNSEIDDLLNGRQI
                     EELGLYSGFFNNGTLLDYFPEDGLVISYRNIDAMNSVWNVEERANQLRMVKENRGEIP
                     LNFPTDRISWNEIEASIEKLKKNIFLMPWGASDLDNNNTRPMPFSSPPLYKGDLNALS
                     VDTSEFTSGGGSVIAITSHHSRLKEILKGLNPSEPPGTGSVTDLDPLSVLPIDTANIG
                     DGFVINTPQHRLTLLGDKEIFGIAKRRRAKRINKASRETFFEEISPGDYVVHVEHGIA
                     RFVGTDSKHKDSGNHEYLILQYAQGDKLYVPMQHLDRVAPYVAPMDRSPSLTRLGTQE
                     WSRTKARVEKSTREMAMELLSIYAARQVSKGHATGPDTKWQFELEESFPYEETVDQIS
                     TLVEIKDDMESDRPMDRLICGDVGYGKTELALRAAFKTVMDGKQVAVLVPTTVLAQQH
                     YETFSERMNAFPVKLSVLSRFKSNIAQKETLKALMDGTVDICIGTHRLVQKDVRFKDL
                     GLVIIDEEQRFGVVHKERLKQMRSQVDILTLSATPIPRTLHMSLAGVRDMSTIESAPE
                     ERLPIKTYVSEFSDELIREAILREMDRQGQVYFLHNRVYNIEYMSEYIRTLVPDAKIG
                     IAHGQMSEKALEKSMIAFSQGETDVLLCTTIIESGLDIANANTLIVNRADSFGLAQLY
                     QLRGRIGRSSKRGYSYLLIPKSQTMTESAERRLKAMLSATELGSGFKIAMKDLEIRGA
                     GNILGSEQSGHIHAVGFELYTRLLATAVEDLRSQDIPPDDPNHPSSPQPNTVSIDLGI
                     PTGISEDYIEDLPSRLDIYQKLIQCRTLDETDLLERELKDRFGPLPWQALNLLFTLRL
                     KIKASLTGMEYIRKTGEKLVIQFPFEVGGMRFALQNLIGPGWTIGNMQIRSTLIELGD
                     NWEESLVEVVEKLGEFQSRMSDELIKSTAST"
     gene            complement(5875..6594)
                     /locus_tag="QF838_09135"
     CDS             complement(5875..6594)
                     /locus_tag="QF838_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024811.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HJO60880.1"
                     /translation="MIKAIFFDLYGTLAGFKPSRYEIQSQACDKFGISLTQQGVLKGY
                     GQADAFMTEQNKVHPLRQMSETERFNFFCEYEMRVIRGSGIDVDLETAGAIWNTVRTI
                     PYDMTIFEDVWPVLDELRSRDMCLGLLSNMNRLGSELLDEFNLSQHMQFAVTSLELGV
                     EKPNPLMFKKALEVAGVSELEAVHVGDQIGSDVDGAENSGVRPILIDRDGNHSGFSRC
                     LRISNMGQLLEALYPLSEDFK"
     gene            6647..7651
                     /locus_tag="QF838_09140"
     CDS             6647..7651
                     /locus_tag="QF838_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HJO60881.1"
                     /translation="MKAGKLPIEHLERVLDNLKSPDPRVVAGPTPGEDASLIDMGDRY
                     LVATTDPITFTTNHIGWYVVNVNANDVAVMGGEPKWMMTTILLPENYNENSIARIFQD
                     ITEACQQLNVSVIGGHTEITVGIDRPLISGVMLGEVKKGSEIKSSGANIGDSIILTKA
                     IPIEGCAILANEAGTKLKENGIAESLIYESQNLIYDPGISVVKDASIAIESGKITAMH
                     DVTEGGLAGGLMELSYASNLGMHIIREDIPEIPQAKQFSDLMGLDVLGMIASGSLLIC
                     ANPKYDSTILRNLNNNGIKATKIGVMKDLAYGLKISENEHIHDLPKFETDEIARFLSN
                     "
     gene            7702..8565
                     /locus_tag="QF838_09145"
     CDS             7702..8565
                     /locus_tag="QF838_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO60882.1"
                     /translation="MNGTYIKSESDHGVLILTMHDPKTRNAIGLEMMAELEEELDRFE
                     SSTALRALVLTGSDPSFCSGANVKTMNADNESAEVNTIPNDRTPWDLLNEAWDKQAAL
                     NSRPRDLIEGVRYVPLRLHNLQKPSIAAVNGSAIGLGMGIALSCDIRFASTNAKFSEM
                     FVRRGLIPADGSCWQLPRMIGLGKTFMLQYTGEIVSAEESLELGIVNKMTPHDQLMNE
                     TMDLAHRIASGATYSMALIKKLIHESLHTNLEESLKLAGPAQDIARQTSDHKEGVKAF
                     VEKRKPNFTGR"
     gene            complement(8575..9396)
                     /locus_tag="QF838_09150"
     CDS             complement(8575..9396)
                     /locus_tag="QF838_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60883.1"
                     /translation="MTGDIRRQIKFNHTGIQLNCSQWGDKSNPSMLLLHGLQDCGRSW
                     EVFAQSMSSEYWVVALDSRGHGDSDYAPGHYTFTDYVSDVEATISKLGIENPILIGHS
                     AGGRYAFSYAAQNKNHVKALVVVDIDPDPINKPSGSMLERYKSESDQWDSFEDVVDRI
                     WSRQPQSSEDMVRFQAQVMTIPKTEGGWVWKRDRNLIYEYERPDLWETWREIDCPTVI
                     IRGRQSTLLTHETAVKMREELADCKLAELEGGGHWFYQESPGSFESTVKWFLNSI"
     gene            complement(9393..10013)
                     /locus_tag="QF838_09155"
     CDS             complement(9393..10013)
                     /locus_tag="QF838_09155"
                     /inference="COORDINATES: protein motif:HMM:NF020123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HJO60884.1"
                     /translation="MRSTIQGNTNVVKGIAILVLAILISLFTVFLATGLSNRTSATGR
                     SGEQLIGRKSPEFTAAGINGDQVSLSNYFGSPIVLNFWASWCPPCREETPHFEKIWRL
                     HPQKDVVVLGINVQDTLADADKYLTEFDVTFTNARDKDGKIMVDYGVTGLPVTFFIDR
                     EGIIIGRWVGSIGESSLKSRVESLENDSFQNIYGSDKNQDGFRRFN"
     gene            complement(10128..>10336)
                     /locus_tag="QF838_09160"
     CDS             complement(10128..>10336)
                     /locus_tag="QF838_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO60885.1"
                     /translation="PTARESFGTTFMTGDYAKIAEGLGAVGITVTKPEEIASALEQAK
                     QLNANGKTVLIDVHSNMEDRRSNF"
BASE COUNT         3166 a         2171 c         2141 g         2858 t
ORIGIN      
        1 gggctttcta gcccgattat ataagaattc acagtgttaa aattccctga tggaattgtt
       61 gaaacgcgct gggtcgtgga cacctaaaac cactgtttac tatggttgga tagttatagc
      121 agtcggagct atgggaactt atgccgcttc cggatcagct caagttaccc ttgcgggaat
      181 tcaaactctc atgtttgagg atacgggttg ggacagaagc actatcgcac ttggtataac
      241 tataggcact tgggtcgctg gcctgctaac ccctgtattt ggaaaaatcg cagatactca
      301 cggcccaagg ttagtaatgc cattaacctc cgtaatcgtt ggtgtgtgtt tttactggat
      361 cggaggcatg tctgcaattt ggcacttcta tgccgcctac ataattgcaa gagggttagg
      421 taaccctgtt ctgatagggg ttatgcctcg aacggtggcg gtgaattttt ttgagagaaa
      481 aagaaatctg gttctcggca tagtatcgat ggcaagacct tgctttggag ccataaatgt
      541 acaactaatc acactgataa gtacatggtc tagctggcgg acggcctata aattacttgg
      601 gatgtattcc atattattag cgctaccttt aataattttt gttagaaaag accctgaatc
      661 aatagggctt tttcccgatg gagttaaaaa aaatcttagc tctctggaat cggctgaaaa
      721 tcctgaaaaa gactcccgta acgatgggga gatttggtca gcaggcgaag cagcaaagac
      781 ttttgcatta tgggcagtga tatccgctga atttttaata atcctgacat ccggaaccat
      841 aggatttcaa cttgtacctt acctacatga atctggatta tccatatcca tggcagctct
      901 tgcctggact cttagcaccc ttttgaacgc gttttctaat ccaatatggg gatttttatc
      961 tgatatatat tctccgcgaa aattgatatt atctgccctc cccatgtcct taggggtaac
     1021 ttccattttt ctactcatag aaggtgggta tcctggtttt gcctgtgtgg ttatttgggg
     1081 ggccgccacc ggaggtctca atgtactggg aggtatgata atagcgaact attatggtcg
     1141 acattctttt ggttcgattt caggaattat gggtcctttt cagatcattg gtctagggtt
     1201 aggacccata ataggtgcta tcctatatga caaaacagga gggtacgaac aactgtttat
     1261 ttttgcgata attgcctacc ttttcgctac tatcttgttc ttttttgcca gacgacccat
     1321 gctgaaatta tccaaaagaa cccaaaaaac tttgatatga aatcaatcca aatacatttt
     1381 tgtgtttcat ttatttttct tatcctattg ggtgtttcct gcaaccaaaa tgtgaataat
     1441 aattcggaaa tttctccaaa tgaagctcct tcatttcaaa aagcccgcat tgccttcgct
     1501 gtgagtgatt tgagtgttgg aactcaccga cttgcttttg ggatccttga gcccggcata
     1561 ggagcagtca aaaatgagga aattaagctt gaaattttct tttttcaaaa cgatgatcgg
     1621 cccatattaa aggaaacttt atccgccaaa tttcaaaagt ggcctattgg agataaaggc
     1681 gtttacacca gtaatgtaac ctttgacgta ccgggcaaat ggggagtagg agtatccttc
     1741 aactccacgg atggcattgt aaaaaagacc tcatcggtca tacaagtaac tgagaaaagc
     1801 caggctcctt caatagggga caaggcattc ccatcaaata atagtactat caatatttca
     1861 cgaaatctca gctacataac aactgacagc gctccttaca aaccttttta tgaatactct
     1921 atcgccgaat cactcaatga aggaaaagcc attctaattt tctttggatc ccctgcattc
     1981 tgtacaactg gtacctgcgg accacaaatc gatattgtta aaagtttaca ttcagatttc
     2041 tctgaatact tgatcttcat acatgttgaa atttatgaaa atccgacgga attagaaggg
     2101 gatatatcaa atgccaaaat tgtgcaagcg gttaaggatt ggaacttacc cagcgagcct
     2161 tggatattcc tagtagacca agaaggtgtg ataaaaagta gatttgaagg tttagcaact
     2221 tatcaagaaa ttgaaatcgc tttaatcgaa aataatttcc tcatttcgaa gtgatggtaa
     2281 catttaagaa agaaggccgg tcctgcgtgg gatcggtcat ttgtagttag aacatatttg
     2341 ataacaaaat ggatcttata aaaataacag agcttttgaa atctgacccc aactatgaag
     2401 gattggtgtc tgaaatcatt aagggtaaac ctgaaacccg tgttcagatc atagacgagg
     2461 ctgtaccttt ttttaccgca tctctatgga aagatttgca aatacctatt ttgcttatat
     2521 gcccaagtcc ggaacattcg agacggctac atgaatattt atccgcttgg atggacgggg
     2581 aatcaaaacc acttaggttc ggtgaaagtg aaattttgcc atatgagaga ttgacagaag
     2641 atctctccac atctcgagaa agactaataa ctttagacag cttggtaaag ggtaattcag
     2701 aaccactggt tatagtaact tcagcgtcag cccttgccca aaaaacaatt tcaatcaaat
     2761 cgtttaaaaa aaattccttc tcctatgtac agggagacaa agtaaatcta gaagacatgg
     2821 ccaattcctg gcagcaaatg ggttactctt ttgaatcaaa tgtgtcagac cctggaactg
     2881 tcagtagaag agggggaatt atagacgtat atccgatagg tgaggatacg ccatatagaa
     2941 tagaactttg gggtgatgaa atagatagta taagaacttt tgatccagac agccaaagat
     3001 caataaatat tgtcgaccga ttctccatcg gtccagctaa agagacttta atatcatcaa
     3061 cctccgcgga tgaatttgat cgactccttt ctcacgtgga tctttcgcga tgtagtcctg
     3121 acgaaataac tcgatttaat tctgaaattg atgacctgtt aaacggtcgt caaattgaag
     3181 aattgggttt atattctggg tttttcaata acgggacctt gcttgattat ttccctgagg
     3241 acggattagt catttcatat aggaatatcg atgcgatgaa ctccgtatgg aatgttgagg
     3301 aacgggccaa tcaattaagg atggtaaagg aaaatcgagg agagattcca ctaaattttc
     3361 ctaccgatag aatttcttgg aatgaaatag aagcctcaat agaaaaacta aagaaaaaca
     3421 tatttctgat gccttgggga gcatcagacc tggataataa caatactcgc ccaatgccat
     3481 ttagttcccc tcccctatat aaaggtgacc taaacgcctt atcggtagat acgtctgaat
     3541 tcacaagtgg tgggggtagt gtgattgcta tcacctctca tcattcaaga ttaaaggaga
     3601 tcctaaaggg tttaaacccc tctgaacctc caggcaccgg ttccgtaact gatttagatc
     3661 cgctatcagt acttccgatt gacactgcta atattggaga cggatttgta attaatacgc
     3721 cacaacatcg tctaaccctt ttaggtgata aagagatatt tggcatcgcc aaacgcagac
     3781 gggcgaaaag aatcaacaaa gcatcaagag aaactttttt tgaagaaata tcccctggtg
     3841 attacgtggt ccatgtggaa cacggtatag ctagatttgt cggtacagat agcaagcata
     3901 aagactccgg taaccatgaa tatttaattc tccagtacgc tcagggggat aagctgtatg
     3961 tcccgatgca gcacttagac agagttgccc catatgtggc tcctatggat agatctccct
     4021 cactcacaag actggggact caagaatggt ctcggacaaa agctagagtt gagaagtcaa
     4081 ctcgcgaaat ggcgatggaa ttgctctcta tatatgctgc aagacaggtg agtaagggtc
     4141 acgctactgg tcccgacaca aaatggcaat tcgaattgga agaatctttc ccctatgaag
     4201 aaactgtaga tcaaatttcc actttagtag aaataaaaga cgacatggaa tctgatcgac
     4261 ccatggaccg actcatatgc ggggacgttg gatacggtaa gacggaattg gctctaagag
     4321 cagctttcaa aaccgtaatg gatggaaagc aagttgctgt gttagtacca accacagtcc
     4381 tagcacaaca acactatgaa actttttcag aaagaatgaa tgcttttcct gtcaaattat
     4441 ctgtattgag cagatttaaa tccaatatag cccagaagga aactttaaag gccttaatgg
     4501 atggaacagt tgatatatgc attggcacac accgactagt tcagaaagat gtcagattta
     4561 aagatttagg cctagtaata atagatgaag aacaacgttt cggggttgtg cataaagaac
     4621 gacttaaaca aatgcgaagc caagttgaca ttctaaccct ctctgccaca ccgataccaa
     4681 gaactttaca tatgtcgttg gccggagtca gagacatgag cacaatagaa agtgccccag
     4741 aagaaaggct accaataaaa acatatgttt ctgaatttag tgacgagctg attagggagg
     4801 cgatacttag agaaatggat cgtcaagggc aagtgtattt tttgcacaat cgggtttata
     4861 acattgaata tatgtccgaa tatattcgaa ccttggtccc agacgcgaag ataggcatcg
     4921 ctcatggtca gatgtcggaa aaagcgctag agaaatcaat gatagccttc tcacaaggtg
     4981 aaaccgatgt cttgctctgt acaacgataa tagaatctgg gttggatata gccaatgcaa
     5041 acacattgat agtaaaccga gcagattcat ttggattagc acagctttac cagttaagag
     5101 gaaggatcgg taggagttct aaaagaggat attcatatct actcatacct aaaagtcaga
     5161 ccatgacgga atcggccgaa agacggctaa aggcaatgtt atccgcaaca gagcttggtt
     5221 caggcttcaa aattgctatg aaggacttag aaattcgggg ggccggaaac atattaggaa
     5281 gcgagcaaag tggtcatatc catgctgtag gttttgaact ttacactagg cttttagcaa
     5341 cagctgttga agatttacga tctcaagata tcccaccaga cgatccaaat caccccagtt
     5401 ctccccaacc taatacggta agcatagatt tgggaattcc taccggaata tctgaagact
     5461 atatagaaga tcttccttca cgtttagata tatatcaaaa attgatccaa tgccgcacat
     5521 tggatgaaac tgacttatta gagcgggagt taaaggatcg gttcggccca ctaccttggc
     5581 aagctttaaa ccttcttttt actcttcgcc tcaagattaa agcatcgtta actggtatgg
     5641 aatacattcg gaaaactgga gaaaaattag ttattcagtt tccttttgag gtgggcggaa
     5701 tgagatttgc actgcaaaac ctaattggtc ctggttggac tattggtaat atgcagataa
     5761 ggtccacttt aatcgaactt ggtgacaatt gggaagaaag tcttgtagag gtagtcgaaa
     5821 aattaggaga atttcaatca agaatgtctg atgagttaat caaatccaca gcatctactt
     5881 aaaatcttct gacaaaggat ataaagcttc caatagctgt cccatattgg aaatcctcag
     5941 acagcgagag aacccagagt gatttccgtc cctgtcaatt aatatcggcc gcacaccaga
     6001 gttttcagcg ccatcaacat ctgaaccaat ctgatcaccg acatgaactg cctcaagctc
     6061 agagacaccg gcgacctcca aggctttctt aaacattaaa ggattcggtt tttcaacacc
     6121 caattctaag gaagtcacag caaattgcat atgttgcgac aagttaaatt catccaatag
     6181 ttcgcttccc aaccgattca tattcgacag taatcctagg cacatatccc gagatctcaa
     6241 ttcgtccaga acaggccaaa catcctcaaa aatggtcata tcatatggaa ttgtcctaac
     6301 ggtattccat attgccccgg ctgtctccaa atcgacatct atgcctgagc cccttataac
     6361 tctcatttca tattcacaga aaaaattaaa ccgttcagtt tctgacattt gccgcagcgg
     6421 gtgaacttta ttctgctccg tcatgaaggc atcagcctga ccatagccct taagaacacc
     6481 ttgttgagtc aacgaaatac caaacttatc gcaggcctgc gattgaattt catatcgcga
     6541 tggtttaaat cccgccaaag tcccatacag atcaaaaaaa atcgctttta tcatatcgca
     6601 gctataatag cacctctata tctcaaagca gcgatggatt gtccttatga aagctggtaa
     6661 gttgccaata gaacacttag agagggttct agataacctt aagtcaccgg atccacgggt
     6721 cgttgcagga ccaacccccg gggaagatgc ttccttaatt gatatgggag atcgatatct
     6781 tgtggcaaca accgacccaa taacattcac aacaaaccac attggttggt atgtcgtaaa
     6841 tgtaaatgcc aatgatgtgg ctgtaatggg gggtgaaccg aaatggatga tgacaacgat
     6901 acttttacct gaaaattata atgaaaattc tatagcccgc atctttcaag atattacgga
     6961 ggcatgccag caattaaatg tatccgttat cggtggccat acagaaatca ccgtaggaat
     7021 tgatcggcct ttgatatcgg gagttatgtt aggagaggtt aaaaaaggca gtgagataaa
     7081 atcttcaggg gcaaatatag gagattcaat tatcttaact aaagccatcc cgatagaagg
     7141 ttgtgcgatc ttggccaacg aggcaggaac taaattgaag gaaaatggca ttgcagaaag
     7201 cttaatttat gaatcacaaa acctcatata tgacccgggc atcagcgtgg ttaaggatgc
     7261 gtctatcgca atagaatcag ggaaaataac tgccatgcat gacgtcacag aaggtggtct
     7321 cgccggtggt ctaatggaac tttcatatgc ctctaattta ggtatgcaca taattagaga
     7381 ggacattccg gagatacctc aagctaaaca gttctcagac cttatgggcc ttgacgtact
     7441 tggaatgata gcctcagggt ctttattgat atgtgcgaac ccaaagtatg actctactat
     7501 attaagaaac ctaaataaca atggaataaa agcaactaaa atcggagtaa tgaaagattt
     7561 ggcctatggg ctcaaaatat ctgagaatga acacatacat gatctaccaa aatttgagac
     7621 tgatgaaatc gctcggtttc tgtccaatta atcacaacaa gtcaaaactc attaagtaag
     7681 catttcaagt ggaggcataa gatgaatgga acctacataa aatctgaaag cgatcatggt
     7741 gttctaatac taaccatgca tgaccctaaa acaagaaacg ccataggact ggaaatgatg
     7801 gcagaactag aagaagagct cgacagattt gagtcatcta ctgcactccg tgcattggtt
     7861 ttgactggat cagatccctc attttgctcc ggagccaatg tcaaaaccat gaatgcggat
     7921 aatgagtccg ccgaggttaa caccatacca aatgatcgca cgccatggga cctcttaaat
     7981 gaagcctggg acaagcaagc agcattaaat tcaagaccgc gggacctgat agagggtgtc
     8041 cgctatgtcc ccctgcgatt gcacaatcta caaaaacctt caatagccgc cgtgaacggg
     8101 tcggccattg gtctgggaat gggcatagct ttatcttgcg acatacgttt tgcttctacc
     8161 aatgctaagt tttctgagat gttcgtcaga agagggttaa tacctgcgga tggttcctgc
     8221 tggcagctac ctcgtatgat aggtttggga aaaacgttca tgcttcaata cacaggagaa
     8281 atagtttcag ctgaagaatc tttagagctc ggtatcgtga acaaaatgac accgcatgat
     8341 cagttaatga atgaaacgat ggacctggcc caccgaatag cctccggggc cacctattcc
     8401 atggctttga taaaaaaact tattcatgaa tcactgcaca ccaacctgga ggaaagttta
     8461 aaactcgccg gacctgctca agatattgcc cgtcaaacat ccgaccacaa agagggcgtg
     8521 aaagcatttg ttgagaaacg aaaacccaat ttcacaggcc ggtaacagct gttcctatat
     8581 agaattaagg aaccatttaa cggtggattc aaaggaacca ggggactctt gataaaacca
     8641 gtgtcctcct ccctcaagtt cagccaactt acagtcggcc aactcctctc tcattttcac
     8701 agcagtttca tgggttaaga gtgtgctttg cctacctctg attatgacag tgggacagtc
     8761 aatctctctc caagtttccc aaagatcagg tcgttcatat tcgtagatga ggtttctgtc
     8821 tctcttccat acccatcctc cttcagtttt tggtatggtc attacttgag cctgaaatct
     8881 gaccatgtct tctgaagact ggggttgtct ggaccatatt ctgtcgacaa catcttcaaa
     8941 actatcccat tggtcagatt cgcttttgta cctttctaac attgaaccag aaggcttatt
     9001 aattgggtcc ggatctatat cgacaactac caacgcttta acatggtttt tgttttgagc
     9061 agcatatgaa aaggcatatc tgccgccggc tgaatggcca attaatatcg gattttcaat
     9121 gcctaatttc gatatagttg cctccacatc ggatacataa tctgtaaatg tgtaatgccc
     9181 aggagcatag tcgctgtcgc catgccctct actatccaga gccaccaccc agtattcgct
     9241 agacatggat tgagcaaaca cttcccaact cctaccgcag tcttgcaggc cgtgcaacaa
     9301 aagcatcgaa ggattagatt tatcacccca ttgcgaacaa tttagttgta tgccggtatg
     9361 attaaatttg atctgcctac ggatatcgcc cgtcaattga atctcctaaa tccgtcttgg
     9421 tttttatcag agccgtatat attctgaaat gaatcatttt ctaatgactc aacccttgat
     9481 ttcaaacttg actccccaat tgaaccaacc catcgaccta tgataatacc ctcccgatct
     9541 ataaaaaaag tgactggtag tccagtcact ccatagtcca ccattatttt tccgtcttta
     9601 tctctagcgt tagtaaatgt gacatcaaat tccgtaaggt atttatcagc atcagccaag
     9661 gtatcttgca cgtttatccc cagcactacc acatcttttt gaggatgcaa tctccagatt
     9721 ttctcgaaat ggggtgtttc ttctctgcaa ggtggacacc aagatgccca aaaattgaga
     9781 acaattggtg acccgaaata atttgataaa gatacttgat ccccatttat accagcagca
     9841 gtaaattcag gtgacttcct tcctatcaat tgctcaccac tccttcccgt tgcagaggtt
     9901 ctgttagaaa ggcctgtggc gagaaatacc gtaaatagcg atataagtat agccaatact
     9961 aagatggcaa ttcctttcac aacattagta ttgccttgaa ttgttgatct cataacactc
    10021 gggaatatga attttcttac gtaagaagca tgcttcaact taatattata aagtgaaaag
    10081 ggggcgtcta aaagactccc ccttattaca attatcagga tacgcgatta aaaatttgac
    10141 cgtcgatctt ccatatttga gtggacatct ataagtaccg ttttaccgtt cgcattcaat
    10201 tgttttgcct gttccaaggc tgaagctatc tcctcaggtt ttgtcaccgt tattccaacc
    10261 gctccgaggc cctcggcaat cttggcataa tcaccagtca tgaaagttgt gccgaaagat
    10321 tcccttgctg ttgggt
//
LOCUS       DASCNS010000255         7949 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001928714, whole genome shotgun sequence.
ACCESSION   DASCNS010000255 DASCNS010000000
VERSION     DASCNS010000255.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001928714"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="QF838_09165"
     CDS             <1..717
                     /locus_tag="QF838_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60886.1"
                     /translation="SGSKIWNSAHADSDWMFMLVRTEPDAPKHRGISFVLTELEGQEG
                     VTIEKIPMMWDAYRGLVTFDNVRVPKKNLVGEQNRGWYVGAALLDFERSGVDRPARAK
                     RVLEDLVEFCKENKKNGRLLADDPVIRNELAELAVQVETTRLMCYNVAWMQSQDLIPN
                     KEASITKVYGTETVNRLYGLGVKIMGLYGQLDPDSKWAPLKGRIENAWLRSFGGMVAA
                     GTSEIQRNVIAQRGLELPRG"
     gene            764..1888
                     /locus_tag="QF838_09170"
     CDS             764..1888
                     /locus_tag="QF838_09170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJO60887.1"
                     /translation="MDLGLNEAQQMLKTTAQDFLSAECPDTYVREMEEDDRGYTPELW
                     TKIAEQGWLGLIIPEQYGGVELEFQDLVVLLEEMGRYMLPGPFFSTVVLGGMSIMDAG
                     SEEQKQEYLPRIAEGQIIMTLALTEPSARWDAAGVELTATSDGDSYTLKGTKLFVQNA
                     HVSDYMVVAARTGNSDEDISLFVVPTQSDGIEQTLLKTIASDRQSEVVFNDVKVPASA
                     LLGEENKGWETIQKVMQWGAIGKCAEMSGGGQQVLDMTVDYAKQRTQFGRPIGSFQAI
                     QHHCANMATDVEGSKFITYQAAWRLSEGLPADREVAMAKAWVSDAYRRVCALGHQSHG
                     AIGFTKEHNMQLYSRRAKASEIMFGDTDFHLENVANIIGL"
     gene            1963..2859
                     /locus_tag="QF838_09175"
     CDS             1963..2859
                     /locus_tag="QF838_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO60888.1"
                     /translation="MKTQIEHVVCTDVFKIFKVADLEVVALRGLDLTVYESEVVALVG
                     ASGSGKSTLLNILAGYDSPSAGQVTIAGKDLINMKGSEIEKFHREEIGFVWQQASRNL
                     FPYLTSLENVALPMLLSTTSDSERKNRSEHLLNMVGMEHRSDHIPDQLSGGEQQRVAI
                     AVGLANDPSLILADEPTGELDDRTASEILDLFGKVNQESKTTIVIVTHDPDIAYKVGR
                     VVVIKDGKTSSEIHRRDLDSKISGEMDKTKPLEESLLIDSNGRVQIPRELLQTTGITN
                     KVKAVAENTSIRIQPPEESPTE"
     gene            2868..3578
                     /locus_tag="QF838_09180"
     CDS             2868..3578
                     /locus_tag="QF838_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO60889.1"
                     /translation="MTDQKPLLELINVSKSYVSESENINPLRDINIKIYEGQFIAIVG
                     RSGSGKTTLLNVMAGLDKPTSGDILIKGSNMTAMDENSLTEIRRTTIGFIFQSFGLLP
                     LLSAFENVELPLRISGVEHGERSNRTKEALDIVGLNPRSNHRPYELSGGEQQRVSIAR
                     AIVTKPAIILADEPTGELDSTNAKSIFGLFKDMVVEQGMTIIAATHDSSLLSMADEVK
                     EIRNGGLYEVQSEDQRYR"
     gene            3716..4072
                     /locus_tag="QF838_09185"
     CDS             3716..4072
                     /locus_tag="QF838_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60890.1"
                     /translation="MNNKNYRPELKGRLDRYVSKSILASPGNTNKKHETVITCYSYHC
                     PLSRKCNIKTQRPSKVSIRKCLRFHPEETKPFGTLSELALDLLSAAEQRGEKLSKRTK
                     SILDQYRPTGPLRQGL"
     gene            4105..4872
                     /locus_tag="QF838_09190"
     CDS             4105..4872
                     /locus_tag="QF838_09190"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HJO60891.1"
                     /translation="MSENLFDLHGKTALITGGNGGIGLGIAKGFAQSGANVAIAGRNT
                     VKTEEVVANLSEQNTSAIGIEVDVADEKSVDNMITNTLENFGQIDILVNNAGIGIRNL
                     PHEYNLEDWNKVIDINLTGAFLCSKAVYPNMKTRGSGKIINIGSMTSIFGLDWAIAYA
                     SSKGGIVQLTKTLAVSWAKDGIQANSILPGWIHTDLTQGIKDNYKDRYDHILSRIPEN
                     RWGEPADLSGTAIFLASDASNYVTGVSIPVDGGYTSF"
     gene            4892..5671
                     /locus_tag="QF838_09195"
     CDS             4892..5671
                     /locus_tag="QF838_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO60892.1"
                     /translation="MSEPIKFNIENQIARVTFNRPEQHNAISYDGWLKLIEIINKIAE
                     DSSIRSVVFSGNGGQAFSAGADIKDFETHRKDSKTAKTYSTAFDGALDAIEALPVPTI
                     SMIRGICVGGGCELSMATDIRIASNRSKFGIPVAKLGILVGYREMKRLVNLVGPGNAS
                     YILLSGRLIDSAEAFRMGLITQLVEDSELEETVLNLANEITPLAPLSQKRHKIILQNV
                     INNQSLDGLDQELPFTNFDSADFKEGKEAFISRRPPKFKGR"
     gene            complement(5697..7814)
                     /locus_tag="QF838_09200"
     CDS             complement(5697..7814)
                     /locus_tag="QF838_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HJO60893.1"
                     /translation="MPIPIEEISKFPLPGLDFPSSFSFSPDGKYLAYLKSVGSSGYKA
                     LYGVDLANKSEFLIADNLGDSSAKETLEEQLRRQRLRQMSGGISQYFWTKNSKILIPH
                     QGNIYWLDDPFSTPDLLMSASDHPCLDVKASPNGNFISFVQNGDIHLIEVKSRTSFKL
                     TEGEDDSTRGVADYIAQEEMGRSTGYWWSADSCYVAFTEVTEKHIPEFLINHVGSDSV
                     GSNAIESHRYPFAGQDNPGVRVGIVEVDSKNCVWVESTEDEYIARVDWFPDNSLAIQT
                     ESRDQQHLKIRKFSIEDSTLTTVLEEDSETWINLHNTMKPITSGRFLWASERTGFNHI
                     YLFCEGDLLELTQGEWQVDSIESVDELGGTIFFTGTKDGHTEKHLYKMSLEGEGLEKV
                     TSESGIHNVSINVKANLFIDIFNSLSSPPTVILRSLGKNYDVEMVIHEPADERIDRYQ
                     LEPPEIFELRLQDGTELSGALYYPNADKFKAPYPTVLSVYGGPHVQLVTNSWMMTASL
                     RAQYLRDKGYLVVVVDSRGSSRRGLKFESPIKNLMGKVEVEDQSFALNHLIEQGIVDK
                     ERIGVYGWSYGGYMSLMCLAKRPDLFKAAVAGAPVTSWDGYDTHYTERYMGMPEENKA
                     GYSKSSVMSYVSEIEGKLLLVHGLVDENVHFRHTARLINALNEAGKDYDLLVFPEERH
                     MPRRLEDRAYMERRILEFLQDNV"
BASE COUNT         2538 a         1652 c         1649 g         2110 t
ORIGIN      
        1 agtggttcaa agatctggaa ttcagcacac gcagattctg actggatgtt catgctggtc
       61 cggactgaac ccgatgcccc aaaacataga ggaattagtt ttgtactgac agaactggaa
      121 ggtcaggaag gggtaaccat agagaaaatt cccatgatgt gggatgcata ccgtgggcta
      181 gttacatttg ataatgtgag ggttcctaag aaaaacctcg taggcgaaca gaacagaggg
      241 tggtacgtag gagccgcact attagatttt gaaagatcag gtgttgacag accggcaaga
      301 gcaaaaagag ttcttgaaga tctcgtggaa ttttgtaaag agaataaaaa gaatgggaga
      361 ttgctggcgg atgacccagt gattcgaaac gaactggcag agctagctgt tcaagtcgaa
      421 acaactcgcc taatgtgcta taacgtcgct tggatgcaaa gccaagatct catacctaac
      481 aaagaagcct ccataacaaa agtatatggg actgagacag taaataggct gtatggactc
      541 ggagtaaaga tcatgggcct atacggtcag cttgacccag attctaaatg ggcacctctg
      601 aaaggccgaa ttgaaaatgc ctggcttcgt tcattcggtg gcatggtagc tgctggcact
      661 tcagaaatac aaagaaatgt tattgcacag agggggttgg aattacctcg aggttaaatc
      721 ctcccatatt ccaacaaatt tttaatatca caggagacta ggaatggatt taggattaaa
      781 tgaagcacaa cagatgctta aaacaacagc acaggacttt ctctcagcag aatgcccaga
      841 cacatatgta agggagatgg aagaggatga ccgaggctac acccctgagc tctggaccaa
      901 aatcgcggaa caagggtggt taggtctcat aattccagaa caatatggtg gagtggagtt
      961 ggaatttcag gatctagtag tactcctgga ggaaatggga cgctacatgc tccccgggcc
     1021 ttttttctca actgtagttt taggcggaat gtccataatg gacgccggca gcgaagaaca
     1081 gaaacaggaa tatttgccaa ggattgctga aggtcaaatc atcatgacat tggcattgac
     1141 cgagccaagc gccagatggg acgccgcggg agtagaactt acagcaacat ccgacggtga
     1201 ttcctacaca ctcaaaggga ccaaattatt tgtacaaaac gcccatgtat cggattacat
     1261 ggtggtagct gccaggacag gaaactcaga tgaagatatc agcctttttg tagtcccgac
     1321 acaatcggat ggcatagaac aaacacttct taagaccatc gcttcagata gacaatctga
     1381 ggttgttttc aacgatgtaa aggtccctgc atctgcctta ctaggagaag aaaacaaggg
     1441 atgggaaacg atccaaaaag tcatgcaatg gggcgctatc ggtaagtgtg ccgagatgtc
     1501 tggcggaggc caacaggttt tagacatgac agtggactat gccaaacaga gaacccaatt
     1561 tggacggcct ataggaagtt ttcaggcaat acagcaccac tgcgcaaata tggccacaga
     1621 tgttgaaggt tccaaattca tcacttacca agcagcctgg cggttgtcgg aagggctacc
     1681 tgcagatagg gaagtagcga tggccaaagc ttgggttagc gatgcttata gaagagtctg
     1741 cgcacttggc catcaatccc acggagcaat aggattcacc aaagagcata acatgcaact
     1801 atactctcgg agggctaaag catctgaaat catgtttggt gacacagact tccacttgga
     1861 aaatgttgcc aatataattg gcctgtaaaa gcgtcattaa tggtacttta aaaagaaccg
     1921 ttgccggact ggcaacggtt ctttttattt ataatgtcta taatgaaaac acaaatagag
     1981 catgttgttt gtacagatgt attcaagata tttaaagtcg cagaccttga agtagtagcc
     2041 ttacgtggtt tagatctcac tgtgtacgaa tctgaggtag tggcactagt cggggcaagt
     2101 ggtagcggta aaagtacatt gctcaacata ttggcgggat atgattcccc ttctgccggg
     2161 caagttacca tagcgggtaa agatcttatc aatatgaaag gtagtgaaat tgagaagttt
     2221 cacagagaag aaatcggttt tgtatggcag caagcctcaa gaaacctctt cccctattta
     2281 acatcgttag aaaacgtggc actgccaatg ctactttcaa caacctccga ttcagaacgt
     2341 aaaaacagat ccgaacatct attaaacatg gtcggcatgg aacatagatc agatcacatt
     2401 ccagaccaac tctctggagg ggaacaacag agagtagcaa tagctgtagg attagcaaat
     2461 gacccgtctc tgatcctagc tgatgaacca actggagagc tagatgatag gaccgcatct
     2521 gaaattttag atttgtttgg gaaagtaaac caggaaagta aaacaactat tgtcattgtt
     2581 actcatgacc cagacattgc ttataaggta ggcagagtcg tcgtaatcaa agacggcaag
     2641 acctcatcag aaattcaccg ccgagatttg gatagcaaaa tcagtggtga aatggacaaa
     2701 acgaagccgc tagaggagtc tttgttgata gattctaacg ggagagtcca gatacccaga
     2761 gaactgttgc aaactactgg cataaccaat aaggtcaaag ccgttgctga aaatacctcg
     2821 ataagaatac aacctccgga agaatcacct actgaataga atatctaatg actgatcaaa
     2881 aaccgctcct agaattaata aatgtatcca aatcatacgt ttcagaatcc gaaaacataa
     2941 atcctttacg agacattaat ataaaaatat acgaaggcca attcattgct attgtgggaa
     3001 ggtctggatc aggtaaaaca acccttttga atgtcatggc aggattagat aaacctacta
     3061 gtggggatat tttgattaag ggctcaaata tgactgcaat ggatgaaaat tcattaaccg
     3121 aaattaggag aactacaatc ggtttcatat ttcaatcatt tggattactt ccactgcttt
     3181 ctgcttttga aaacgtggaa ctcccactga gaatatctgg agtggagcac ggtgaacgat
     3241 ccaatagaac aaaagaagct ctcgatatag ttggcctcaa tcctaggtct aaccacagac
     3301 cttacgagct ttctggcggt gaacaacaaa gagtctcaat tgccagggct atcgtaacca
     3361 aaccagccat aattttagcc gatgaaccga ctggggaact tgattcgaca aacgctaaat
     3421 ccatatttgg tttatttaaa gacatggtgg ttgaacaggg aatgaccata attgctgcaa
     3481 cacatgattc ttcactacta tccatggcag acgaggtaaa agaaataaga aatggaggtt
     3541 tatacgaagt tcaatctgaa gatcaaagat atcgttaaat aaaaccttta agggaatcca
     3601 ataatatatc tttagtgact cgtttattgt agtatcagtc tacccctctg tttaaatgct
     3661 aaattgctta tctgccagtg cggctcctta tattagttat caataagagt tgcaattgaa
     3721 taacaaaaac taccgacccg agttgaaagg gagacttgac cgatatgtct ccaaatccat
     3781 attagcatca ccgggtaata ccaataaaaa acatgaaact gtcatcactt gctattctta
     3841 ccattgtccg ctgtcaagaa aatgtaacat caaaactcaa agaccttcca aggtttccat
     3901 aaggaaatgt cttagattcc accctgaaga aaccaaacct ttcggcactt tatcggaact
     3961 tgccctcgac ttattatctg ccgcagaaca gcggggagaa aaacttagta aaagaacgaa
     4021 atcaatactt gatcaataca gaccaaccgg tccactaaga caagggttat aatcgcgaaa
     4081 tacttattat tgctaggaga agttatgtcg gagaatttat ttgacttaca tggtaagacg
     4141 gccctaatta caggcggtaa cggtggaatt ggattgggaa tagccaaagg ctttgctcaa
     4201 tcaggtgcaa acgtagcaat agcaggaaga aatactgtaa aaacggaaga agtggttgcg
     4261 aatttgagtg agcaaaacac atccgcaatt ggcatagaag ttgatgtagc cgacgaaaaa
     4321 tcagtagaca acatgattac aaatacctta gagaatttcg gacaaatcga tatattagtc
     4381 aacaatgctg gaattggaat acgaaattta cctcacgaat acaatctaga agattggaac
     4441 aaagtaattg atataaattt aaccggggca ttcctctgct cgaaagcagt ttatccaaac
     4501 atgaaaaccc gaggttcagg aaaaataata aatatcggat ctatgacctc gatatttggc
     4561 ctagattggg ccatcgcata cgcctcgagt aaaggaggta tagtgcagct taccaaaacg
     4621 ctggcagtat catgggcaaa agatgggata caggccaact ctatattacc tggatggatt
     4681 cacacagacc taacccaggg aattaaagat aattacaaag ataggtatga tcatatactt
     4741 tccagaattc ctgagaatag atggggggaa ccagcagacc tttccggcac tgcgattttt
     4801 cttgcaagcg acgcttccaa ctatgtaaca ggtgtctcta tcccagtaga tggcggatat
     4861 acctctttct aaccccttat aaaggtaacc aatgtcagaa ccaattaaat ttaatatcga
     4921 aaatcagatt gctcgagtga cttttaacag gcccgaacaa cataacgcta tcagctatga
     4981 tggctggttg aaattaatag aaattattaa caaaatcgca gaagattcat caatccggtc
     5041 agtagttttc tccggtaatg gaggacaggc cttttcagct ggagctgata tcaaagattt
     5101 tgaaacacat cggaaagatt caaaaaccgc taaaacttat tcaaccgctt ttgatggagc
     5161 tctcgatgct atcgaagctc tacccgtgcc tactatttcc atgattcgtg ggatatgcgt
     5221 tggtgggggc tgtgagctat ctatggcaac tgacattcgc atagcatcta atagaagcaa
     5281 gtttggtatc cctgtagcaa aattgggtat attggttggc tacagagaaa tgaaaagact
     5341 agtaaatttg gttgggcctg gcaatgcctc atatattctt ctgagtggaa gactaattga
     5401 tagcgcggaa gcattccgta tgggtttgat aacacagcta gtagaagatt ctgaacttga
     5461 ggaaactgtc ttaaatttgg ccaacgaaat tacaccgctt gctcctcttt ctcaaaagag
     5521 gcacaaaatc attttgcaaa atgtaattaa taaccaatcg ttagatggat tagatcaaga
     5581 gctcccattc actaattttg atagtgccga ttttaaagag ggcaaagagg ctttcatatc
     5641 tcgcagaccc cctaaattca agggtagata gacaagataa ggggctgata taagttttag
     5701 acgttatctt gtagaaactc caaaatacgc cgctccatgt atgctctgtc ttcaagacga
     5761 cggggcatat gtctttcttc tggaaaaacc agcagatcat aatctttacc ggcctcatta
     5821 agagcattta ttaggcgagc agtatgccta aaatgaacat tctcatccac gagaccatga
     5881 accagcaaaa gctttccttc aatttcagaa acatatgaca taacactact tttgctataa
     5941 cctgctttgt tctcttcagg cattcccata tatctttcag tgtaatgagt atcataaccg
     6001 tcccaactag tcaccggtgc ccccgcgact gcagctttaa acaaatctgg tcgcttggct
     6061 aaacacatta aagacatata gccaccatag ctccacccat agacccctat tctctcctta
     6121 tcgactatcc cttgctcgat taggtgattg agcgcaaacg attgatcctc aacttccact
     6181 tttcccatca agtttttgat tggagattca aattttaatc cccttctgga actacctctg
     6241 ctatcaacca ccactaccaa gtatccctta tcccttaaat actgagccct cagtgaagct
     6301 gtcatcatcc atgaattagt aaccaactga acatgtggtc caccatatac actaagcact
     6361 gtgggatacg gggctttaaa tttatctgca tttggataat ataaggctcc tgaaagttca
     6421 gttccatctt gtagtcttaa ctcaaagatt tccggcggtt ccaattgata tctatcgatc
     6481 ctttcatctg caggttcatg tattaccatt tccacatcat agttctttcc taacgaccgc
     6541 aatataactg ttgggggaga agacaaagaa ttgaaaatat ctatgaacaa attagcctta
     6601 acattgatag atacgttatg aatgccgctt tcagacgtga ccttttctaa gccttctcct
     6661 tccagagaca ttttataaag atgtttttcg gtgtgaccgt ctttggttcc tgtaaaaaaa
     6721 attgtgccac ctaattcatc cacagattcg attgaatcca cctgccattc cccttgggtc
     6781 aactctagta gatctccttc acaaaaaaga taaatatgat tgaatcctgt tctttcacta
     6841 gcccataaaa atcgacctga ggtgatgggt ttcatcgtat tatgtaaatt aatccaggtc
     6901 tccgaatcct cctccaggac tgttgtcagg gtagaatctt caattgaaaa tttacggatt
     6961 ttgagatgct gttgatcacg tgattcggtt tgaattgcca atgaattatc tggaaaccaa
     7021 tctactctgg ctatgtattc atcttcagtg gattccaccc acacacaatt ctttgaatct
     7081 acttctacaa tcccaactct gacgccagga ttgtcctgtc ccgcaaatgg atatctgtga
     7141 gattctatcg cgttagatcc taccgaatcg gaaccaacat gatttataag aaactccgga
     7201 atatgtttct cggtaacttc tgtaaatgcc acataacaac tgtctgcaga ccaccagtac
     7261 cctgtgcttc tacccatttc ttcttgagct atataatctg ccacacctct ggttgaatcg
     7321 tcttccccct cagtcaactt aaaagaggtt ctagatttaa cttctatcaa atgaatatca
     7381 ccattttgga caaaagaaat aaaattccca ttcggggaag ccttaacatc taaacatgga
     7441 tgatctgaag cggacatcaa tagatctgga gttgaaaaag gatcatctag ccaatatata
     7501 tttccttgat ggggtattaa tatttttgag ttttttgtcc aaaaatattg ggaaatccct
     7561 ccagacatct ggcgtaacct ctgcctacgg agctgttctt ccagagtttc ttttgcagaa
     7621 gaatcaccta agttatccgc aatcagaaat tccgatttat tagctaaatc taccccatat
     7681 aaagctttat atcctgatga acctactgat tttagatagg ccagatactt tccatcagga
     7741 ctgaaggaaa aactgctagg aaaatccagt cccggcaggg gaaatttgga aatctcttca
     7801 ataggtatag gcattaacgt attcgctgaa ctacctttct tgcagagttc actaattggt
     7861 taacatgatt ttcatcagaa aacacgttta aggctcctgc tgctcccgac tgcaacaaaa
     7921 ttccttcatt cagcatgccg aagtaaaaa
//
LOCUS       DASCNS010000256         5533 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001930005, whole genome shotgun sequence.
ACCESSION   DASCNS010000256 DASCNS010000000
VERSION     DASCNS010000256.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001930005"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="QF838_09205"
     CDS             <1..416
                     /locus_tag="QF838_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO60894.1"
                     /translation="ENRNKNNVCIIGIESVPAIENLENMLKVEGIDAIFVGPNDLTIT
                     LGIPDQYDHPDYEAALREIIAKSEAAGKPVLIHHQTVELTQKWLREGARFVLYSSDAR
                     TMHNGYREEFGEIKSVGSELGGETIGAVGESEEVI"
     gene            429..1328
                     /locus_tag="QF838_09210"
     CDS             429..1328
                     /locus_tag="QF838_09210"
                     /inference="COORDINATES: protein motif:HMM:NF020039.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HJO60895.1"
                     /translation="MSWDFIAVQGPFGGTSEGPAWDGESLLFTHIPESKIYRFNPRTS
                     TTEVFRENTNCANGLMFDESGNLYACEGGGRRVVRYDGDSTEVLAESYQGSRFNVPND
                     LAIDPQGRVWFTDPFYEGAGGPWSQDRANKELDHDSVYRIDMADGKSTAVSRVTEDTT
                     RPNGLLFSLDFQTLYVAQSGRLPEEKRQLRAYPVNTDGSLGDHEVIHDFGEHRGIDGM
                     VLDADGNIVATAGYKAGGPGPMIYVFSASGEVLETHSTPFDRPTNCSFGGEDLSTLYV
                     TTGDGFLLRAFTERQGRLSYPKI"
     gene            1361..2545
                     /locus_tag="QF838_09215"
     CDS             1361..2545
                     /locus_tag="QF838_09215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJO60896.1"
                     /translation="MNADVIVIGAGIAGCSAALHLSESGMSTLLLDMGEVSGEASGVN
                     MGGLGGAGWGNSPKLQEYLTMGSLNIFKRLQIELGYDLEFRQSGSFTAIHNEQQYSYA
                     QNRVIQSQKDGFNLRLLSSYEAKAIEPELNPDLLGYVYAPTRGQADPLKTTRAFGQAA
                     MQAGTKVQTNTQVIGMRSHHGIWDLETNNGNYEAQFVVIAAGAWCKNIGEMVGANIPI
                     EPVVGQMWSTDPLPPRVFSTISSFESSFHWSENPYVDSISPPELTHFGEQRVTRHLYG
                     RQRKNGEIIFGGDRRDAGFNKTVDAGGVEINKGQATEVLPLLSGLPISTTWSGLMPFS
                     KDGQPVIGRIPANDNLFVVTGLCSSGFGKGPGAGMLIADLVSRGESHPVLAESDPARF
                     SA"
     gene            2619..3668
                     /locus_tag="QF838_09220"
     CDS             2619..3668
                     /locus_tag="QF838_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HJO60897.1"
                     /translation="MTGESIKIGLVGAGGNTTLRHIPGLNAMDNVVIDSVCNRSMASG
                     KRVADEFGIEKVYDNWIDVMEDPDNDAVCIGTWPYMHCTLVLAALENGKHVQTEARMA
                     INASEAHIMLEASKNFPGLVTQIVPAPHTLNLDKTIKTLIKDGYLGDILSVDAAITQG
                     GFVDKDKPYHWRQSRDFSGYNIMGLGIWYEAVMRWIGPAATVQAITRVTVPQRPDQNG
                     DLQVLTIPDHVEILCEMFTGPVLHIRFSDVLGHAPGNGVWIYGTEGTLHVDATNQADL
                     KLFGGRKSDSSLSEIAIRPELTGGWRVEEEFINAIRGIEEITHTSFEDGVRYMEFTEA
                     VTRSSQSGEKIYLPL"
     gene            3691..4884
                     /locus_tag="QF838_09225"
     CDS             3691..4884
                     /locus_tag="QF838_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HJO60898.1"
                     /translation="MAYNYQQLASTNLPAEIEMPPGLREEVEYVFSVTYTDPDAMALK
                     EFAEAAATVIERDGRSLGTYPPPQGHEGLREFISSELSSNRGSDIPADNIFLASGAGG
                     ACQVFVDAFIDPGDVVMMEEFCYHGSLNMFIKKGARPCHVSMDQDGMIPEELEKSIQE
                     NIKNGDKPKLIYTISVYHNPTGATINLERRKRIVEISNKYQIPIVENESYADFHIDGP
                     QLPPAMIGLDSDGGVMHISAFTKLMGCGLRLGFGAFPEAARESLAKVGFGVSPSHLTS
                     MAVHEYLKDNKSSYVSGVASSLGRKRDAMLRSLGEYFPPSCTWTEPSGGMMIWMGLPE
                     GTNTWDILEKAVEKGVKYNPGPIFRADRRGGNHLRLTFSHHTPQEIEEGIALLAGVFE
                     AEGVI"
     gene            4886..5185
                     /gene="gatC"
                     /locus_tag="QF838_09230"
     CDS             4886..5185
                     /gene="gatC"
                     /locus_tag="QF838_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HJO60899.1"
                     /translation="MSEQIDLSIEEIRQISVLTRIAMTEDELDVMRAQMSNILDSVAI
                     LNEVDTEDVEPTGHSVNIKSVMREDQIQDSLTQDETLLNAPRRDGSFIRIKAVLE"
     gene            5204..>5533
                     /locus_tag="QF838_09235"
     CDS             5204..>5533
                     /locus_tag="QF838_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HJO60900.1"
                     /translation="MESVNLETITIAEARRLLDNKEVSAVELTKNYLDRIKNFEKDID
                     AYVTVTEDRALEDANLADKRIGEGTQTNLTGIPMQLKDNICYSGIPTTCSSKMLENFV
                     PPFDASVS"
BASE COUNT         1643 a         1013 c         1428 g         1449 t
ORIGIN      
        1 tggaaaatcg aaataagaat aatgtttgca taataggtat tgagagtgtc ccagctatcg
       61 aaaacttgga aaacatgctt aaagtagaag gaattgatgc aatttttgtg gggccaaacg
      121 atctcacaat aaccttaggg ataccggatc aatatgacca tccagactat gaagctgctc
      181 tacgggaaat aattgcaaaa agtgaagctg ccggtaaacc ggttttgatt catcaccaaa
      241 cagttgaatt gactcaaaaa tggcttcgag aaggagcaag gtttgttctt tattcgagtg
      301 atgccagaac tatgcataac gggtatagag aggagtttgg agaaataaaa tcagtaggat
      361 ctgaactagg tggtgagacc ataggtgctg taggcgagtc agaagaagta atttaggagg
      421 aagaaataat gagttgggat ttcatagcag tacaaggacc atttgggggt accagtgagg
      481 gcccagcgtg ggatggtgaa tctcttttat tcacccacat tcctgaaagc aaaatatata
      541 gatttaatcc cagaacgagt accaccgagg tttttagaga aaatactaat tgtgccaatg
      601 gattgatgtt tgatgaatct ggaaacctgt atgcttgcga aggaggagga aggagagtcg
      661 tcaggtatga cggtgactca acagaagttt tagcggaatc atatcaaggc agcaggttta
      721 atgttccaaa tgatttggcg attgatcctc agggaagggt ctggtttaca gatccttttt
      781 atgaaggggc aggtggccct tggagccaag atagggcaaa caaggagttg gaccatgact
      841 ctgtctatag aatagatatg gcagacggga agtcaacagc tgtgagccga gtgaccgagg
      901 atactactcg gccgaacggt cttctgtttt ccctagattt tcaaaccttg tatgtggctc
      961 aaagtggccg attaccggag gaaaaaagac agctacgagc ttacccggtt aatacggatg
     1021 ggtcattagg cgaccatgag gtaatacatg attttggcga acacaggggc atagatggga
     1081 tggtgcttga tgcagatgga aatatcgtgg ctaccgctgg atataaggcg ggcggaccgg
     1141 gccccatgat atacgtattt tccgcctctg gagaggtgct ggaaacgcat tctactccgt
     1201 ttgataggcc aactaattgt tcatttggcg gtgaggattt atcaaccttg tatgtcacga
     1261 caggtgatgg gtttttgtta cgggctttta ctgaacgaca aggacgactt agctatccaa
     1321 agatatagtg ccattgttat gaaaatggga gtgaagtaat ttgaatgctg acgtgatagt
     1381 aataggtgcc ggtatcgcag ggtgttctgc agcccttcat ttgtctgaat caggaatgtc
     1441 aactttgctt ttggacatgg gggaggtttc gggggaagct tctggagtca atatgggagg
     1501 acttggtggt gcaggatggg gtaattcacc taaattgcag gaatacctaa caatggggag
     1561 cctgaacatt ttcaagcgac ttcagattga gttgggctat gatttggaat ttagacagtc
     1621 agggtctttc acggcaatac ataatgagca gcaatattcg tatgcccaga accgggttat
     1681 acaatctcag aaagatggct ttaatctgcg gttattgagt tcctacgagg cgaaggcgat
     1741 agagccggaa ttgaacccag atttgcttgg atatgtttat gctcctacga gaggtcaggc
     1801 tgatccacta aagaccaccc gggcatttgg gcaggctgct atgcaggcag gcactaaagt
     1861 gcaaacaaat acccaagtaa ttggtatgcg atcacaccat ggaatatggg atctagagac
     1921 aaataacggg aattatgagg cacagttcgt cgttattgct gcaggggctt ggtgtaaaaa
     1981 tataggtgaa atggtaggcg cgaatatacc tatagaacca gttgttgggc agatgtggtc
     2041 tacagacccc ttgccgccca gggttttcag tacaatatcg tcattcgagt ctagtttcca
     2101 ttggagtgaa aatccatacg tagacagcat ttctccacct gaattaaccc attttggaga
     2161 acaaagagta accagacacc tatatggaag acaacgcaaa aatggagaaa ttatctttgg
     2221 aggtgatcgt cgagacgccg gtttcaataa gacagtggac gctggagggg tggaaattaa
     2281 caaaggacag gctacagaag ttcttccttt gttatccgga ttaccaatat cgacgacgtg
     2341 gtctggattg atgccatttt cgaaggacgg ccagccagtt atcggcagga taccagcgaa
     2401 tgataattta tttgtggtaa cagggctttg ttcttcgggc ttcggaaaag gacctggcgc
     2461 tggtatgttg atagctgatc tagtttctcg tggtgaatct caccctgtac tggcggaatc
     2521 ggacccagct cgattctctg cgtagactgc gaatcacgac tcaaatataa tccatattca
     2581 atatgatatt ttaagggaaa actgaagagg taccaattat gacaggtgaa agcataaaaa
     2641 tcggacttgt tggggctggc ggaaatacga cacttaggca tatacctggg cttaatgcca
     2701 tggataatgt agttatagac agtgtttgta atagaagtat ggcatctggg aaaagggtcg
     2761 ccgatgaatt tggtatagag aaagtttatg acaattggat agatgttatg gaggaccctg
     2821 ataacgatgc tgtatgtata ggaacttggc catacatgca ctgcaccctg gtactcgccg
     2881 ctttggaaaa cgggaaacat gtgcagaccg aggctagaat ggcaataaat gcctcagagg
     2941 ctcatatcat gttggaagct tctaagaatt tcccgggtct tgttacccaa atagttccag
     3001 caccccacac cctaaattta gataagacca tcaaaactct tattaaagac ggatatctgg
     3061 gggacatatt gtcggtggat gctgctatca ctcaaggagg atttgtagat aaggacaagc
     3121 catatcactg gcgccaaagt agggatttta gtggctataa catcatgggt ctgggtatct
     3181 ggtacgaggc tgtaatgcga tggataggcc ctgcggctac ggttcaagca ataaccagag
     3241 taacagtacc tcagcgacct gatcaaaatg gcgatttgca ggtgctgacg atacctgacc
     3301 acgttgaaat cctgtgtgag atgtttacag gcccggtttt gcatattagg ttcagcgatg
     3361 tcctgggtca tgctccggga aatggtgtat ggatttatgg tacagaaggt actttgcatg
     3421 tagatgccac aaaccaagca gacctcaaac ttttcggtgg gagaaagtca gattcttccc
     3481 tttctgagat agctataagg cccgagttga ccggtggatg gagggtagaa gaggagttta
     3541 taaatgcgat tagaggcatc gaagagataa cccacacatc atttgaagat ggtgtccgct
     3601 atatggaatt taccgaagcc gtgacccgta gttctcagtc gggcgagaaa atataccttc
     3661 cactctagtt agaatttgga gcatcaagaa atggcgtata actatcaaca attagcttct
     3721 actaatctgc cagcagaaat agagatgccc ccaggattaa gagaggaagt tgaatatgta
     3781 ttttcggtaa cttatacgga tccagatgct atggcactca aagaatttgc tgaagcagcg
     3841 gcgacggtta ttgaaagaga cgggagatct ttaggtacat accctccccc acaagggcat
     3901 gaagggctga gggaattcat atcctctgaa ttgtcttcca atcgaggttc agatattccg
     3961 gcagataata tttttctagc cagtggggcc ggaggtgcat gtcaggtttt tgttgatgcc
     4021 ttcattgatc ctggtgatgt agttatgatg gaggaatttt gttatcacgg tagcctgaat
     4081 atgttcataa aaaaaggggc ccgtccttgc catgtatcca tggatcaaga tgggatgatt
     4141 ccagaagagc tagaaaagtc gatccaagag aatattaaga acggtgataa acccaagctc
     4201 atttatacga tttctgttta ccataaccct actggtgcca ctattaatct tgaaagaaga
     4261 aagaggatag ttgagatatc taataaatat cagataccta tagtagaaaa tgaatcctat
     4321 gctgattttc acattgatgg gccacaatta cccccagcaa tgatcgggtt ggattcagat
     4381 ggtggagtca tgcatatttc ggcatttacc aaattgatgg gctgcgggct taggctaggg
     4441 tttggtgcct ttccagaagc tgctcgggaa tcgctggcaa aagtaggatt tggcgtatcc
     4501 ccgagtcatt tgacttccat ggctgtacat gaatatctaa aagacaataa atcttcatat
     4561 gtctctggtg tagcttcctc cttgggtcgg aaacgtgatg cgatgctgag atcgttaggt
     4621 gagtatttcc ctccctcttg tacttggact gagcccagcg gtgggatgat gatttggatg
     4681 ggacttccag aaggaacgaa tacctgggat attttggaga aggcagtaga aaaaggggtg
     4741 aaatacaacc ctggcccaat attcagagca gataggaggg gggggaatca tttacggctt
     4801 actttcagtc accatacacc tcaagaaata gaagaaggaa tagctctttt ggctggcgtt
     4861 tttgaggcag aaggagtcat atagaatgtc cgagcaaata gatttgtcaa ttgaagaaat
     4921 taggcagata tctgtgttga ctagaattgc catgacagaa gatgagttgg atgtgatgcg
     4981 ggctcaaatg tctaatatat tagacagtgt agcaatcctc aatgaagtgg atacagaaga
     5041 tgttgaacct accggacatt cagtaaacat aaagtcagtg atgagagaag accaaataca
     5101 agactctttg acacaggatg aaactctttt aaatgctcct cgaagggatg ggtctttcat
     5161 tagaattaag gctgttctgg aatagggata cccccgacta ggcatggaat ctgtaaacct
     5221 cgaaactatt acaattgctg aagctaggcg gcttcttgat aacaaagaag tgtcagcagt
     5281 tgaactgacc aaaaattatc tcgatagaat taaaaatttc gaaaaagaca tagatgcata
     5341 tgtcacagtc acggaagatc gagccctaga agacgctaac ttggcagata agagaatagg
     5401 tgagggaact caaaccaacc tcactggaat tccgatgcag ttaaaagata atatttgcta
     5461 ctcaggcatt cccacaactt gctcctcaaa aatgttggaa aattttgtac cgccatttga
     5521 tgcatcagta tcg
//
LOCUS       DASCNS010000257         3194 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001942147, whole genome shotgun sequence.
ACCESSION   DASCNS010000257 DASCNS010000000
VERSION     DASCNS010000257.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001942147"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            164..838
                     /locus_tag="QF838_09240"
     CDS             164..838
                     /locus_tag="QF838_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJO60901.1"
                     /translation="MPEIVISTENLHKFYGSRDQKVHALKGLDISIEEGEIVGIMGPS
                     GCGKTTLLNCLSGLDSFDEGNISIQGGTLTDLNDDQLSELRAKKMGFVFQTYNLLPVL
                     NAVENVELPLLVSGFSGKEARSKALEQLAEVELEDWQHHLPGELSGGQRQRVTLARAL
                     VNDPAIVWADEPTGNLDSENERLIMDLLTQLNKENNQTFVIVTHSDYVGGRTDRIIQM
                     KDGEIV"
     gene            843..>3194
                     /locus_tag="QF838_09245"
     CDS             843..>3194
                     /locus_tag="QF838_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HJO60902.1"
                     /translation="MEEVFGLPAKRLALGMTVFLVVIFLVIGIGAARRFILVKMGSRN
                     IPRRKGQSTLIVIGLMLSTAIIATSLGIGDTVRYSVRSVALDFLGPTDEIIKGPGRQL
                     IGEEYFDFSQFQNIEQVTRDNKNIEALLPIIEINLPASNDVLELAESNMRVRGVHGEY
                     SDNYDELQNLAGQTVSINALLDNEIYINADSSRILKLQKGDRVSVYSRSGKFEFSIRD
                     VLKNGGLAGGGRNPYILFSLDELQELIGKQGQITEVLVSNSGEGEDSLKLSEDVTKFL
                     RSELTNTDIATKMFGILQGNGIPEIFLEESNSINQTDKETSKTLNQLSGHLSSNDFGD
                     DFIKEIGDYQNQLTILGILDKSGLKEDAGKIAALSPTLTTLRVDDQKSDSVRLAETVA
                     TGVTTIFSIFGSFSIMVGMLLIFLVFVLLAAARSTELGMARAVGLKRRDLVQLFTYEG
                     TIYAFLAAIVGTVVGVGLSVGLVFILQDLIDSDEFVITPYYSVVSLLIAFSTGLILTF
                     ITVVFSAYRASNLNIVVAIRGLKDEFVKKAPDSIGKKARDFVWNLIFPVRQLVWIIRG
                     NGGRMRNFGLLLLFPVVWPINILTSLFRLSGKHNYLVPGIFSMLLLLYGISTEVGASL
                     SIGLIGSALTVGLFARYLCSKFINDTETGNQIAGTLEGGLVLLSNSLPYDFFSRWLPD
                     LSEPGPWFWPVGGAISTAAAVWLLMSNTRILIALLNLILSRFSGLKAVTKTAISYPMA
                     AKFRTGLTVAMFGLIIFTLMIFSVLNGIQDVATDKTGRVTGGYD"
BASE COUNT          962 a          546 c          739 g          947 t
ORIGIN      
        1 gagtcaaaga agatgaaatt gaatagtaca ggcctcaagg ttgttagcag attaacctag
       61 taacaatttg ccttgattta tcccttcata tttttgtgtt attaagggac aatataaagg
      121 tagatatcaa tttgagacct cgctagtcgg gataggaaat catatgccag aaatcgtcat
      181 ttctactgaa aacctccata aattttatgg ttcaagagat caaaaagttc acgctttaaa
      241 gggattggat atctcgatag aagaggggga aatagttgga atcatgggtc ctagtgggtg
      301 tggtaaaact actcttctaa actgcctgtc cggattggat tcatttgatg aaggaaacat
      361 atccatccaa ggtggtaccc tgaccgattt aaatgacgat caattgagtg aattgagagc
      421 aaagaaaatg ggttttgttt ttcaaaccta taacttgtta ccggttttaa atgcggtcga
      481 aaatgttgag ttgccactac ttgtttcagg attttctggt aaagaggcta gatctaaggc
      541 gttggaacaa ttggcagaag tggagttgga agattggcaa caccatttac ctggtgagtt
      601 atcgggaggt caacggcaga gagttacttt ggctagagca ctggtaaatg acccggctat
      661 cgtttgggcg gacgaaccca caggtaattt agatagcgag aatgaacgac tcattatgga
      721 tctactcacc cagctaaata aggagaacaa tcagactttt gtgatagtaa cccattctga
      781 ttatgtggga ggtaggacag acaggattat tcaaatgaaa gacggggaaa tagtgtaaca
      841 aaatggaaga agtttttggt ttacccgcta aaagattagc cctgggaatg acggtattct
      901 tggtcgtcat ttttctcgtt atcgggatag gggcggcaag aagatttatc ttggtaaaga
      961 tgggaagtcg aaacatccca agaaggaaag gtcaaagcac tttaattgtg atagggttga
     1021 tgctcagtac cgccataatt gctacatctt taggaatagg agatacagtt agatattcgg
     1081 ttaggtctgt agctttagat ttccttggcc ctacggatga gattattaaa ggccccggta
     1141 gacaactaat cggggaggaa tatttcgatt tttcgcaatt tcaaaatata gaacaagtaa
     1201 cccgagacaa caaaaatata gaggctctcc tcccgataat agaaattaat ttaccagctt
     1261 caaatgacgt actagagctg gctgaaagca atatgagagt gcgaggagtc catggagaat
     1321 attctgataa ttacgacgaa ttacaaaatc tggccggtca aactgtatcc atcaatgcac
     1381 ttttagacaa tgaaatttac ataaatgctg actccagtag gattttaaag ctccagaaag
     1441 gagatagggt tagtgtttat agccgttcag gtaagtttga attttcaatt cgtgatgttc
     1501 taaagaatgg tggattagct ggtggaggta gaaacccata catactattt agccttgatg
     1561 aattacaaga attaattggt aagcaaggac agataaccga agttttggtc tcaaattctg
     1621 gcgagggaga ggattcactc aaactttcgg aggatgtcac aaaattccta agatctgaac
     1681 taacaaatac cgacatcgcg acaaaaatgt ttggaatact tcaaggaaac ggtatccctg
     1741 agatattttt agaagagtct aattccatta accaaacaga caaagagacc tccaaaactc
     1801 tcaaccaatt atctgggcat ttatcatcca atgattttgg ggatgacttc ataaaagaaa
     1861 taggcgatta tcagaaccaa ttgaccattc tggggatatt ggacaaatct ggactcaagg
     1921 aagatgccgg caaaatagca gcactttcac caaccttgac cactttgaga gttgatgatc
     1981 aaaagagcga tagtgttaga ttggcggaaa cagtggcaac tggtgtaact acgatttttt
     2041 ctatctttgg atcgttctcc atcatggtag gaatgttact aatcttccta gtatttgtct
     2101 tactcgctgc tgcccgctcc actgaattag gaatggccag agcagtaggt ttgaaacgta
     2161 gggatttagt gcagttattt acatatgaag gcactatata cgcctttcta gcggcaatag
     2221 taggaacggt ggttggagta ggattaagtg ttggattggt atttatcctt caagatttaa
     2281 tagatagtga cgagtttgta atcacaccat actactctgt cgtctctctt ctgatagctt
     2341 tttcaactgg attaatactg actttcatta cggttgtctt ctctgcatat agagccagca
     2401 acttgaacat agttgttgca atcagaggac taaaagatga gtttgttaaa aaggcgccag
     2461 actctatagg caaaaaggca agggattttg tttggaattt aatattccca gttaggcaat
     2521 tggtttggat aattagagga aacgggggtc ggatgagaaa tttcggcctt ttacttctat
     2581 ttccagtggt gtggccgata aatattttga cctcgctatt caggttgtct gggaaacata
     2641 attaccttgt tcctgggata ttctcaatgc ttctgttgct ttatggaata tcgacagagg
     2701 ttggagccag cctttctata gggctgatag gctccgcttt aactgttgga ttattcgcta
     2761 gatacttgtg ttccaaattt ataaatgata cagaaactgg gaatcaaatc gcgggtactt
     2821 tggaaggcgg tctagttctt ctttctaact ctctaccata tgactttttc agcagatggc
     2881 tacctgattt gtctgaacct ggaccatggt tttggccggt aggaggggcg atctcaactg
     2941 cggcggctgt ctggttgcta atgtctaaca ctcgcatttt gattgcactt cttaatttga
     3001 ttttatctag attttctgga ttgaaagcag taacaaagac ggctataagt tacccaatgg
     3061 cagccaagtt taggacaggc ctcactgtag ctatgtttgg tttgatcata tttacactta
     3121 tgattttttc tgtcctaaat gggatacaag atgtggcaac cgacaagact ggaagggtga
     3181 ctggaggcta tgat
//
LOCUS       DASCNS010000258        13899 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001946870, whole genome shotgun sequence.
ACCESSION   DASCNS010000258 DASCNS010000000
VERSION     DASCNS010000258.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001946870"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="QF838_09250"
     CDS             complement(<1..426)
                     /locus_tag="QF838_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_256875649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(256..258),aa:Sec)
                     /transl_except=(pos:complement(160..162),aa:Sec)
                     /transl_table=11
                     /product="FtrB family double-selenoprotein"
                     /protein_id="HJO60903.1"
                     /translation="MPKEMSESEALASAQKFSERYVDRGPYEFFPLKEVVEEVQKGLA
                     ENHRLEGYRYCPUMPLSGDPVEDRKKICPCERHHEDIERDGFCIUMFFVSEEFLRKYE
                     SGEDFAQTIDEAVPLGTELFENTGGKKEFNERRREDNKKK"
     gene            complement(440..757)
                     /locus_tag="QF838_09255"
     CDS             complement(440..757)
                     /locus_tag="QF838_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60904.1"
                     /translation="MEEKEIQALVLNEFDDAVNLRPLNGFKMNFSANPGFKKVFFSAS
                     CDCGTAALLSLEVSETKTDDEITDALPSLVQRIEMQEKSFRKMDCSMHSMMRTGFTPD
                     NVS"
     gene            871..2493
                     /locus_tag="QF838_09260"
     CDS             871..2493
                     /locus_tag="QF838_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HJO60905.1"
                     /translation="MTDNMFYKTLSPISFLDRSAEVYPDKPALSYGDITYTYQDFYDR
                     VNKLAGALLKEGVKKGDRVGFLVPNIPAMFEGHYGPLKIGAVLVAINTRLSAREVSYI
                     LNHSGAKVLVFDSEYAELINQIKDELPEITRFVQVADTFPKSTLVEGPEYEEFLNSAD
                     PGEHRIPLDSESDPVTINYTSGTTGMPKGVLYHSRGAYLSALGEVVESGMNQDSVYLW
                     TLPMFHCNGWCFTWATTAVGATNVCLRRVDPLEVYRLIEEESVTHMCCAPTVLTSMYS
                     IPDAALQDLSGVTIMTAGAPPAPQVIRSMENMGAHVLHAYGLTETYGPITICAIQPDW
                     KGKSSEEVANLKSRQGVAFVVADTGLRVVDAEMNDVKKDGSQMGEVAMSGNMVMSGYF
                     NDEEATDKAFEGGWFHSGDLAVWHPDGYIELQDRAKDIIISGGENISSQEVEKVIMEH
                     DGVLEVTVVGVPDDRWGEVPKAFVMSRDGASVSEEEIITFTRDRIAHFKAPKYVEFGE
                     LPKTATGKIQKYVLRDKEWEGKEKRIQGSSLT"
     gene            complement(2518..3456)
                     /locus_tag="QF838_09265"
     CDS             complement(2518..3456)
                     /locus_tag="QF838_09265"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HJO60906.1"
                     /translation="MPLGETNIKDWLCGPMVAVATPFKEDFALDLDSLTSNIRFMVDK
                     GVVTGNGTLLVGGAGGEHPTMNVDERKSVMAAAVKAANGQVPVLTSIQHTDVRVVLEL
                     AEYASGVGLHGVQLGPTYYYEPSEDDVVRLFQYVSEGSDVNLMIYHTWWEGLTMSISL
                     IDQLSQIKNVNAMKWSAPTVQLYREGLVNFSDKLVIIDNGGEHVWSNVLGAAGFITHL
                     SGFWPEYPSEIWALLKASEYDGVKKRLGDFKWKWREWITKVIQETGGEGPFIKAAMER
                     VGLPAGPPRPPSVRPPEHLMAELDVLMKNAGTPIYK"
     gene            complement(3479..4228)
                     /locus_tag="QF838_09270"
     CDS             complement(3479..4228)
                     /locus_tag="QF838_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60907.1"
                     /translation="MRLKDKVSLITGASNGMGAAEARLFSNEGSHVILADIQDDLGGK
                     LVEEITQSGGLAEFFHLDVTEESNWEKLMEYLKSKFGKLDVLVNNAGISASSPDLLNI
                     EIFDNVMKVNTRGVFLGMKYCLGLMSDNGAGSIVNISSISGLVGQAYVHMGYSGSKGA
                     VRLMTKSAAIQYAEKGIRVNSVHPGVMPPMTTSEMTADPEFRKIMLEDVPMRRVGLVD
                     EVAKAVLFMASDDSSYITGAELPVDGGLTAR"
     gene            complement(4260..5048)
                     /locus_tag="QF838_09275"
     CDS             complement(4260..5048)
                     /locus_tag="QF838_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO60908.1"
                     /translation="MTISTDTILYEKRDGVGYITLNRPDSMNALNRELSAGLREALAE
                     VRDDPEVILGVLIGAGGRAFSAGMDLKERATLDAQGERQVLDPRGAGSFIDMGVYKPL
                     IAAIDGFCVAGGLEVSLQCDIRIATKKSEFGLPEPRWSILAGYGLHNLNRMIPLGESL
                     YMQLTGSRINSDRAYNIGLIQDVVEDREKLIERVDKLAGEIKLCAPLAVQAIKQIVYQ
                     GRNLPVEYSQKLAIPISERISGTEDSIEGPKAFSEKRRPEWKMR"
     gene            5437..5775
                     /locus_tag="QF838_09280"
     CDS             5437..5775
                     /locus_tag="QF838_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60909.1"
                     /translation="MDYELVSILSQTATGIATLAVAAFLSFQLRLQHKESVASAQAEL
                     TNGLGEVVRDIYKDSELTDIYLRGITEYETLLKEEKHRFNIVLWTYFMQIQQLWETKR
                     EEEKVRSYAS"
     gene            complement(6358..6987)
                     /locus_tag="QF838_09285"
     CDS             complement(6358..6987)
                     /locus_tag="QF838_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60910.1"
                     /translation="MNSKVVGIMLILGPILIMGVWIGGLVPDTASISPSEAMTTVLAE
                     KNLAEIGSLIQIFGVIAMFAGLYFLARSLKSDNAVSNQLAEMGGLLLLLVVPIWVVFM
                     GSWTAAIGAAEKFGNDVGTTILASSTMFEAGGMLLIVGLVMLGISLTILKKYKGVIGA
                     LFIIAPVCAFIDVIFDIEALAIIGWMGMFITTLIIGILTTLQKENQPEA"
     gene            complement(7157..7396)
                     /locus_tag="QF838_09290"
     CDS             complement(7157..7396)
                     /locus_tag="QF838_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60911.1"
                     /translation="MNYMRISYLQMINEWNLGVNENNVRYFKGTLGILMGTQGERKYY
                     LTNGRIIVGTVFGLSELVSLGNTVYEELEGSSVPA"
     gene            complement(7452..7724)
                     /locus_tag="QF838_09295"
     CDS             complement(7452..7724)
                     /locus_tag="QF838_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HJO60912.1"
                     /translation="MAAYVIVTHPVKDFDVWKIVFDEFEQLRKEAGELTAVVLRHADD
                     SNIVTVLNTWTSIDAAKAFVASEEIKKGMGEAGVTAPPTFVFANSE"
     gene            complement(7960..8469)
                     /locus_tag="QF838_09300"
     CDS             complement(7960..8469)
                     /locus_tag="QF838_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60913.1"
                     /translation="MKYIDDSLFDGTVAILFQIILKRESPDDLPVTFQGSFFTEEELD
                     PSSPIEVLLNGLERALRMHIYEDEDPTLLKAQLLDAARQLVDIYREREGESDLYSAMS
                     MISVAGDFRRNYVFKFDRKGNKKGFLKRYLRGAKFNQNNVYDANRVENSVQAIKFAFA
                     KIKFARFVG"
     gene            complement(8715..9140)
                     /locus_tag="QF838_09305"
     CDS             complement(8715..9140)
                     /locus_tag="QF838_09305"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HJO60914.1"
                     /translation="MKFRKDVATVITLLCMSWLLAACEAEGTVSSAKGPSTQASQSTQ
                     TQEGELELLEYSWETDEIGMKYLSGVVANNTKYTQTKVAILFAIYDADGLKIGEAADV
                     TDSLRPGEKWEIRAICECFYVVDESAETAELDFLIGFPE"
     gene            9368..9628
                     /locus_tag="QF838_09310"
     CDS             9368..9628
                     /locus_tag="QF838_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60915.1"
                     /translation="MDSYFQSGVLEGKSVNIKYYTVRGRILDLTPDSLPDYYLVIMGS
                     TTSKESFRNMTYPADKASVHLFESSSLIKALNYLAQSASLRP"
     gene            complement(9641..10924)
                     /locus_tag="QF838_09315"
     CDS             complement(9641..10924)
                     /locus_tag="QF838_09315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60916.1"
                     /translation="MSLDPDKSRNRTLRPLFPTGDIFYGWWLVGVAAFLLTLMSLTVF
                     QGLGTILVALERQFGWSRTALSGAFSLARVEGAILGPVEGFLVDRIGTRKMVLIGYIL
                     MGLGFIWLGQVKTLWEFYVSFMIISLGSGLGGWLAVIAMVNNWFTRQRTFAMASAMSG
                     IHFGGLLVPLLAIGIETFEFRGAAIIIGVFLLIVVGPAAKVIRNRPEDIGLQPDGDPE
                     RLSESVLTEDEEPDFTAGQALRTPVFWTLTIMQVASSVAIVTLALHLVPKLTDMGMTL
                     SGAGTVVLTYTIVALPSQFLSGYFADRLPKTLMIAVFLAIQGIAITIIAFADTVLLAY
                     IFALLYGIGFGGRNPLTTAIRGEYFGRKAFATIMGISQFPMNIGMIGAPLFAGYMFDT
                     TNSYVIPFSVFAILTFFGAFLMLFVKKPRKLTLQL"
     gene            complement(11099..11175)
                     /locus_tag="QF838_09320"
     tRNA            complement(11099..11175)
                     /locus_tag="QF838_09320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11139..11141),aa:Val,seq:gac)
     gene            complement(11261..12073)
                     /locus_tag="QF838_09325"
     CDS             complement(11261..12073)
                     /locus_tag="QF838_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60917.1"
                     /translation="MGELDGKVAIVTGAGRLRGIGRAAAEALAKLGADVVVTGTGRSP
                     DSFPDDEKAIGWKDIETVAERVRDIGRRALPLVVDVTNRDDVKRMVDETMKEFGRVDI
                     LINNAAYARAEDRTPILDLNEDTFQKVMDIKVTGTFLCTKAAIKPMIDQGDGGKIVNI
                     SSTAGKRGSANTLAYNGANFAIVGMTQSMAKELGSHNINVNAVCPGAVDTHRMDILGR
                     GNTWSNMADSTPIGRNGTDEEVGDFCAYLCTEAASWIHGQSINQNGGTVMEH"
     gene            complement(12159..12848)
                     /locus_tag="QF838_09330"
     CDS             complement(12159..12848)
                     /locus_tag="QF838_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014305.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJO60918.1"
                     /translation="MQDSDDISIGIIGLAEWEIPENQTGRGLTLQTTRGPINTIVHHD
                     PDKPATRGVIWVGGARGGLDGPAGGLYRIVGEELSPGITSLRIDYREPGELIECVMDT
                     LAGVSFLTGTGHSDILLAGHSFGGAVAIKSAPFSDKVKGVIALSSQTFGATDVSEVSP
                     RPILLIHGEDDIVLSPQCSQTIFDWAEEPKEMIFMPATGHGLTESSEEVIEKVKSWVV
                     NHLYLPGEVPE"
     gene            complement(12860..>13899)
                     /gene="gatA"
                     /locus_tag="QF838_09335"
     CDS             complement(12860..>13899)
                     /gene="gatA"
                     /locus_tag="QF838_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HJO60919.1"
                     /translation="TKNPWDTNRVPGGSSGGPAAAVAASECVFSLGSDTGGSIRQPAS
                     LCGVVGMKPTYGLVSRIGLVAFASSLDQIGPLTKSVEDSALVLQSIAGHDSRDSTSLD
                     VKIPDYFSNLTTDLKGFKIGIPKEYFIEGISPGVEKSILDSVEVLRGLGADVKEVSLP
                     HTKYALAVYYIIAPSEASANLARYDGVKYGFSEKDAETMWAGLEKTRSNGFGPEVKRR
                     IMLGAYALSAGYYDAFYLKAQKVRTLIRQEFEDVFKDVDVLAMPTSPTTAFKIGDKID
                     DPLQMYLNDIFTIPANIAGIPGISIPAGMVNGLPVGLQFMAGTLQETNLFKAAYAYEQ
                     ATEWHNQFPSI"
BASE COUNT         3950 a         3354 c         2705 g         3890 t
ORIGIN      
        1 ttttttcttg ttatcttccc ttctacgttc gttgaattcc ttcttaccac cagtattttc
       61 aaaaagttcg gtacccagtg gtacggcttc atctatggtc tgtgcgaaat cctctccgga
      121 ttcatatttg cgaagaaact cttcgctaac gaaaaacatt catatacaga agccatccct
      181 ttcaatgtct tcatgatgtc tctcgcaagg gcatattttt ttgcgatcct caactggatc
      241 gccgcttaga ggcattcaag ggcaatatct atacccttcc aacctgtgat tctcagctaa
      301 ccccttctgg acttcctcta cgacttcctt caaaggaaaa aactcgtagg ggccacgatc
      361 aacatagcgc tctgaaaatt tttgagcact tgccaaggct tcggattctg acatctcctt
      421 tggcatagct ataaatacct caacttacgt tatctggtgt gaacccagta cgcatcatac
      481 tatgcatact acaatccatt tttctaaacg atttttcttg catttcgatt cgttgaacca
      541 gggaaggaag agcatccgtt atttcatcat ctgttttagt ctcagacact tcaaggctca
      601 ataaagcagc ggtcccacag tcgcaacttg cagaaaaaaa cactttctta aaccctgggt
      661 ttgccgagaa attcattttg aatccattca gcggacgtag attcaccgcg tcatcaaact
      721 cgttcagaac cagggcttga atctcttttt cttccatgga ttcatactaa cctaaaatcc
      781 catatttccc aatatcccgg aaggtgtatt tagagccgtt tgatatctaa tatcagaata
      841 tgatccaatg taccctcagg aggctgaatt atgactgata atatgtttta taaaactctt
      901 agccctatct catttcttga cagatccgcc gaggtatacc cggataagcc cgcgttgagt
      961 tatggcgata tcacctacac ttatcaggat ttttacgatc gtgtaaacaa acttgcaggc
     1021 gcactcctga aagaaggtgt taaaaagggt gaccgggttg gattcctggt tcctaacatc
     1081 cctgccatgt ttgaagggca ttacggccca ttgaaaattg gagctgtgct tgttgcgatt
     1141 aataccaggt tatcagcgag agaagtttcg tacatactca atcattcggg cgccaaggtg
     1201 ctagtctttg actccgagta tgctgaactc attaaccaaa taaaggatga gctacctgaa
     1261 ataaccaggt tcgtacaggt agctgatacc ttcccaaaat ccactttggt ggaaggcccc
     1321 gaatatgaag aattcctcaa ttctgccgac cctggagaac atagaattcc actagactct
     1381 gaatcggacc cagtcacaat taactacact tcaggaacca cgggcatgcc taaaggagtc
     1441 ctataccact ctcgaggcgc ctatttatca gccttgggag aagtcgtaga gagtgggatg
     1501 aaccaagact cagtttatct ttggactcta ccgatgttcc attgcaatgg atggtgcttt
     1561 acctgggcga cgactgcggt gggagcaact aacgtctgcc tccggcgggt ggatccactg
     1621 gaagtgtacc gattgataga ggaagaatct gtcacccaca tgtgctgtgc acccacagtt
     1681 ctcacatcga tgtactctat accggatgct gccctacaag atctcagtgg agtaaccata
     1741 atgaccgcag gtgcgccgcc cgccccgcag gtcatacgaa gtatggaaaa tatgggagct
     1801 catgtcctcc atgcctacgg actgactgaa acatacggcc caatcaccat atgcgctatc
     1861 caaccagact ggaaagggaa gagttctgag gaggtggcaa atttgaaatc tcgacaggga
     1921 gtggcatttg tagttgctga tacgggcctt cgggtggtgg acgctgaaat gaacgatgta
     1981 aaaaaagacg gttcacagat gggtgaggtg gcaatgagcg ggaacatggt aatgtctgga
     2041 tatttcaacg atgaagaagc aactgataaa gcttttgaag gcggatggtt tcacagcggc
     2101 gacctagctg tatggcaccc ggacggatac attgagcttc aagatagagc gaaagacata
     2161 ataatttccg gcggtgagaa catatccagc caagaagtcg aaaaagtaat catggaacat
     2221 gatggagtat tggaagtaac cgtagtgggc gtacctgacg acagatgggg agaagtcccc
     2281 aaagcctttg tgatgtcaag ggacggagcc tcggtgtcgg aagaggaaat aatcactttc
     2341 acaagggacc ggatagccca ttttaaagca cctaaatatg ttgaattcgg agaactgcca
     2401 aagacagcca caggtaagat ccagaaatac gttcttagag ataaggaatg ggaaggaaaa
     2461 gaaaaaagga tccagggatc ttctctcaca tgaagtactg tcgctgtcgt gtcgccttta
     2521 tttgtagata ggagtccccg cattcttcat aaggacatcg agttctgcca taaggtgttc
     2581 aggaggtcta actgagggtg gccttggcgg gcctgcagga agacctactc tttccatagc
     2641 tgcttttatg aaaggtcctt caccccctgt ttcctgaatt actttggtaa tccattcacg
     2701 ccacttccat ttaaagtcac ccagtctctt ttttactcca tcatattcag aagctttcag
     2761 tagagcccat atttcggatg gatattcggg ccaaaaccca ctcagatggg tgatgaatcc
     2821 tgcagcacct agtacattac tccaaacatg ctccccaccg ttatcaatga ttactaattt
     2881 atccgaaaaa ttaactaacc cttctctata aagttgaact gtcggggcgg accacttcat
     2941 agcatttaca ttttttatct gtgacaactg gtctatgagg ctaatactca tagttaaacc
     3001 ttcccaccag gtgtggtaaa tcatgagatt tacatcagac ccttcgctga catattgaaa
     3061 aagtctgact acatcgtcct ctgaaggttc gtaataataa gttggtccta attgaacccc
     3121 atggagccct actccactgg catattcagc aagttccagt acaacacgga catctgtatg
     3181 ctggatactt gtaagcaccg gaacctgtcc attggctgct ttgaccgcag ctgccattac
     3241 agatttacgt tcatccacat tcatcgttgg gtgttcaccg ccggcacctc ctactaaaag
     3301 ggtcccgttt cctgtcacca ctcctttatc aaccataaat cgaatattgc tggtcagcga
     3361 atctaaatcc aaggcaaaat cttccttaaa tggagtcgct acagccacca ttgggccaca
     3421 taaccagtct tttatattgg tctctcctaa gggcatcggt acctcacatt aaatattttc
     3481 atcgagcagt gagaccacca tctaccggta gttctgcacc agtaatataa gatgagtcgt
     3541 ctgaagccat aaaaagtacc gcttttgcta cctcatcaac caatcccacc ctgcgcatcg
     3601 gaacatcttc aagcattatt ttcctaaatt cagggtctgc ggtcatttcc gaagtagtca
     3661 taggtggcat aactcctggg tgaacagaat taacccttat acctttttct gcgtactgaa
     3721 ttgccgcgga tttagtcatt agtctaactg cacctttgga tccactgtat cccatatgaa
     3781 cataagcttg acccaccagg ccagaaatag atgagatatt cacaatgcta ccagcgccat
     3841 tatctgacat aagcccaagg caatatttca ttcccagaaa aacacccctg gtatttactt
     3901 tcatcacatt gtcgaaaatc tcaatattca agaggtctgg tgatgaagca cttatacctg
     3961 cattgttaac caaaacgtcc aacttaccaa attttgattt taggtattcc attagttttt
     4021 cccagttgga ttcctcagtc acatctaaat gaaaaaattc tgccaaaccc cctgattgag
     4081 ttatctcttc caccaatttt cctccgaggt catcttgaat atccgccaaa atcacatggg
     4141 agccttcatt tgaaaaaagt cttgcttcag cagctcccat tccatttgag gctcccgtta
     4201 ttaaagaaac cttgtctttc aatctcatct taacttgcct ccattaattg aagtgtttat
     4261 tacctcattt tccattctgg tcttcttttc tccgagaatg cctttgggcc ctctatagaa
     4321 tcctccgttc ctgaaattct ctctgaaata ggaattgcaa gtttttgaga gtattcgaca
     4381 ggcaagtttc taccctggta aacgatttgc tttatcgcct gtactgcaag aggcgcacac
     4441 agtttaatct ctcccgccaa cttatccact ctttcaatca atttttctct atcttccacc
     4501 acgtcttgta tgagtccaat attgtatgct ctgtcagagt tgattctgct gccagtcaat
     4561 tgcatgtata gagattctcc cagcggtatc attcggttca ggttgtgtaa gccatagcct
     4621 gcaaggatac tccaccttgg ttcagggaga ccgaactccg atttcttggt tgcaattctt
     4681 atgtcacatt gaagggatac ctcaaggccc cccgctacgc agaacccatc aatagccgca
     4741 atcagaggtt tatatacacc catatcgata aatgatccag cccccctggg gtccaacacc
     4801 tgtcgttctc cttgagcatc gagtgtggct ctttctttta aatccattcc tgctgaaaac
     4861 gccctacctc ccgcaccaat caaaactccc agaataacct ctggatcgtc tcttacctct
     4921 gcaagagctt ccctgaggcc agcactcaac tccctgttca gagcgttcat ggaatccggt
     4981 ctgtttaggg ttatatagcc taccccatca cgtttttcat ataaaattgt gtccgttgat
     5041 atggtcattg cattttcctc caaatatttc ttctaccgga ttcggtggaa ccaagtcaaa
     5101 gcatgttatt actcactaat tggcacgaat ttcttaacag tagcagaaag aaatggtagc
     5161 aaacaggggg agcaggcaat agtaggtaga tttacacaac catagctttc ggcactattt
     5221 aggaagcatc aggaagggtc acctcagccc caatcgatgc atcgtggagg gactgagatg
     5281 cccgagcaat tatattaggt gaaggggaca caaatgaacc cttgcaagtt acctatattt
     5341 gagaagcttc atgctccatc atctctactc agcgagcgta attccacaga tttttgaatt
     5401 atttataata acgccacgaa tgattaggag cgcaaaatgg attatgaact agtatcaatt
     5461 ttatcccaaa cagcaacagg tattgcaact cttgcggttg ccgcattttt aagttttcaa
     5521 ttgagattgc aacataaaga gtcagttgca tcagcacaag cagaacttac aaatggattg
     5581 ggtgaggtag tcagagatat ctacaaagac agtgaactta cagatatcta tttgagagga
     5641 ataacggaat acgagacctt actcaaggaa gaaaaacata ggttcaacat agtgctatgg
     5701 acgtatttta tgcaaattca gcagctgtgg gagaccaaac gcgaggaaga aaaagtaaga
     5761 tcctatgcta gctaatacca tgctcaacac agggctggga gttgtggcat ggtggggcaa
     5821 catggggcag tacgtatacc caggagaatt tgtcatatac gtggattccc tcttgaatac
     5881 agactagatt taaaataaag gaaaagccca tgttgctgcc tagtcacaac aatgggattt
     5941 ctccttcgca ccatctctag ttcgcaaaca atggtcaccc cagccccagt ggtgcgttcc
     6001 aacaccggca ctttttacag ggactgagat gcccaattaa tgggactaga ttaatggcaa
     6061 aggcgcagtc ctgataaggc tgcatgggtt tcaatcattt ccttatagcc aacaacaagg
     6121 aagattaaga gtcacgccag ccccaccgca tgtgtagcaa tataggtgag actgaccaaa
     6181 taatagtatc atcccagtcc catttacgag ggtctcctga gcaaattagt tagagtaact
     6241 ctgaaggctt ctgagaccta caggcccatg cgcaggtgaa aatactcttt ctttgattag
     6301 ggtggtaact atttacccta ccttacgcat atcaatccaa ttattcttaa ttaacattta
     6361 cgcttcaggt tggttctctt tttgcagtgt cgttaatatt cctattatta atgtcgttat
     6421 aaacattccc atccaaccta taattgccaa ggcttcaatg tcaaaaatca catcaataaa
     6481 agcacatact ggtgcaataa taaatagtgc tccaataact cccttatatt tttttagaat
     6541 agttagtgat atgcccaaca taactagtcc aactattagc aacattccgc ccgcttcaaa
     6601 catagtagaa gaggctagta ttgttgtacc aacatcattt ccgaattttt cagccgctcc
     6661 tatagctgct gtccatgaac ccataaaaac aacccatata ggtacaacaa gtagaagtaa
     6721 taacccaccc atttcagcca actgattgct tacggcatta tcacttttca atgaccttgc
     6781 tagaaaataa agtccagcaa acatagctat gactccaaaa atctgaatca atgaaccaat
     6841 ttcagctaga tttttttctg ctaagactgt tgtcatagcc tcggatggtg aaatactagc
     6901 tgtatcaggt accaaacctc caatccaaac acccataatt agaattggac ccaatatcaa
     6961 cataataccg acaacttttg aattcatgat tcacctccct aatatgtctt atatgattcc
     7021 acaatgacgt taatctagct aggaattttc agtgttgttt gctccaggat aactcggatg
     7081 caattccatt atccagagga gatacgtctt tccgccacct ctattcagag aggataacca
     7141 cacacaggtt tttaaattat gcaggaacgg aactgccttc aagctcttca tagactgtgt
     7201 tacctaagga aactagttca ctaagaccga aaacagttcc aactattatg cgaccattag
     7261 tcagatagta ttttcgttct ccttgggttc ccattaagat acctagagtg cctttgaaat
     7321 atcggacatt gttttcattt actcctaaat tccattcatt aatcatctgg aggtatgaaa
     7381 ttctcatgta attcataaaa tgatggatag tgtcccgtcg gcactgcctg cacatgttct
     7441 aaatcaatgg gctactctga gttggcaaag acaaatgtgg gtggtgccgt gacaccagct
     7501 tcccccatac ccttttttat ctcttccgaa gccacaaagg ccttggctgc atcgatgctg
     7561 gtccatgtgt taagaacagt cacgatgtta gagtcatctg catgtctcaa gacaactgca
     7621 gttagttcac cagcttcttt tcgaagctgt tcaaactcgt caaaaactat cttccaaaca
     7681 tcaaaatctt tcacagggtg agtaacgata acgtaggcgg ccacattttc tccttatttt
     7741 ggttagcata cctcaccaag tactattttt ctaacccgtt gttaatcctt gaaggggatt
     7801 catcctaaac cgtctctatt caccgaatat agtgcaaccc acacttttga ataatggagg
     7861 actctattca attattagaa tagggttaat actttgtgtc atctaaggag tgtgctcagt
     7921 cacacggatt gaaaataatt ttagtcagat ggaaattttt catccaacaa atctagcaaa
     7981 ctttattttg gcgaaggcga attttattgc ttgaaccgaa ttttctaccc tattcgcgtc
     8041 atatacgtta ttttgattga atttagcccc gcgaagatat cgtttcagaa aacctttttt
     8101 atttcctttt ctatcaaatt taaatacata attacgccgg aaatcaccag ctacagatat
     8161 catcgacatg gctgaatata aatcagattc tccttctctt tctcgataaa tatcgaccaa
     8221 ttgtctagcc gcatctaata attgtgcttt caggagggta gggtcttcat cttcatagat
     8281 atgcatacga agtgctcttt cgagtccatt caaaagaacc tctatggggc tggatgggtc
     8341 taactcttcc tctgtaaaaa aagaaccctg gaatgttact ggcaaatcat ccggagattc
     8401 tctcttgaga attatttgaa agaggatggc cacagttcca tcaaaaagac tgtcgtcaat
     8461 gtatttcatc tgtacgcctt aaataaataa ttccaaccag caaatactgt ctttttcatg
     8521 gggacataat accaatacta ggtgaaaata ctcattcttt tcatcaggag accaaaagac
     8581 cccatcaata aatgtgtttg ggtcatttga gtcaaatcag caatctctgt cagcaggccc
     8641 acacatcgtt gtatgttggt tgagtatgaa ctgaataaaa gcccctccga cgttctagcg
     8701 gtctcctata gatgctattc aggaaagcca atcaaaaagt ccagttccgc cgtttctgca
     8761 ctctcgtcaa ctacatagaa acactcgcaa atggctctaa tttcccactt ttcccccggt
     8821 ctgaggctat cggtaacatc cgccgcttct ccaattttga ggccatcagc gtcatatatg
     8881 gcgaataaaa tagcgacctt tgtttgcgtg tacttagtgt tgtttgctac tacaccactt
     8941 aaatatttca ttccgatttc atctgtttcc caactgtatt ctagcaactc aagttcccct
     9001 tcttgagtct gtgttgactg actcgcttga gttgatgggc cttttgctga agaaacagtg
     9061 ccctctgctt cacaagcagc caaaagccac gacatacaca gcagggtgat aacagtagca
     9121 acatcttttc gaaacttcat atactccact cctctgaaca tgttagtcgt tagacctatt
     9181 gtattgaatg ttgaaaaaga gccaaaggct ttggcattca tttcattcat cctcccatgt
     9241 aaattgtttt ctataggctg aaattggaga aataatagga cgacctgtcc tcacgggtca
     9301 tattggcgaa tacatagctg ccgaaatttt caatatagcc ctctcacatt ctacttctga
     9361 gaagagtttg gatagctact tccaatctgg agtacttgag ggcaaaagtg taaatataaa
     9421 gtactacacg gttagaggga ggattctaga tttaacgcct gacagtcttc cagattacta
     9481 cctcgttata atgggttcaa ccacatctaa agaatcgttt agaaatatga cttatccagc
     9541 cgacaaagca tctgttcatt tatttgaaag ttcctcatta ataaaggcac taaattacct
     9601 cgctcaatcg gcgagtctta gaccataatc aaaaatatca ttatagttgc agtgttagct
     9661 tcctgggttt tttgacaaac aacattagaa aggcaccaaa aaaggtcaag attgcgaaca
     9721 ccgaaaatgg gattacataa ctatttgtag tatcaaacat atagccggca aataggggtg
     9781 ccccaatcat acctatattc attgggaact gggatatgcc cataatcgtc gcgaaggcct
     9841 ttcgcccgaa atattctccc cggatagctg tggttaatgg gttacgacca ccaaacccta
     9901 ttccatacag taaggcaaaa atataggcca aaagtacagt gtctgcgaac gcaattattg
     9961 ttatggcgat tccttggata gcaagaaata cggcgatcat aagagttttg ggaaggcggt
    10021 ctgcaaaata acctgaaaga aactgggacg gcaaggccac tatggtgtag gtaagaacca
    10081 cagtacccgc tccactcaac gtcataccca tgtctgtcag ttttggcact aaatgtaatg
    10141 caagggtgac gattgcaaca cttgaggcca cttgcatgat cgttagtgtc caaaacaccg
    10201 gagttcttaa cgcttgtcca gcggtaaaat caggctcttc gtcttctgtt aggacagatt
    10261 ctgacaacct ctctgggtcc ccgtcaggtt gtagacctat atcctctggg cggtttctta
    10321 taactttcgc cgcagggccc acaactatta agagaaatac cccgattatt atggcagccc
    10381 cgcgaaattc gaaagtttct attccaattg ccaaaagggg aacaagtaac cctccaaagt
    10441 ggatgccaga catggcagaa gccatggcaa atgtacgttg ccgtgtgaac caattattca
    10501 ccatagcgat tacggcaagc caacccccca gacctgagcc aagcgagata atcataaatg
    10561 acacatagaa ttcccaaaga gttttcacct gccccagcca tataaagccc agccccataa
    10621 ggatatagcc tatcaacacc attttccgag ttcctatacg atctaccaag aaaccttcca
    10681 cgggacccag aatggctccc tccacccttg ctagggagaa cgcgccggat aaggcagttc
    10741 ggctccatcc aaattgacgt tcaagagcga ccaaaatcgt acctaaacct tgaaacactg
    10801 taagagacat caatgtcagc agaaaagcag ctaccccaac aagccaccag ccataaaata
    10861 tatctcctgt tgggaacagg ggccttaagg tgcggtttcg acttttatca ggatcaagtg
    10921 acattattct gtttctcttt tgagtggtgg caaacttaat aggacaactg atggcgagta
    10981 taaatggcgt agatgattaa aggaatgtta cgaaaaactt gttttataac cagtcaagaa
    11041 acaaaccacg aaaatggagc aaagaaagga gataattcca tgcccaaccc tagcgggatg
    11101 gtgggcggta taagagtcga acttatgacc tctgcgatgt caacgcagcg ctctaaacca
    11161 ctgagctaac cgccctttat gatgtatctg ttttttctct acgaaggagc aaaagcttag
    11221 caacgtaacc aatctcagtc aatggaatgt gaccccagat ttaatgttcc attaccgtgc
    11281 caccgttctg gttgatggac tgtccatgaa tccatgatgc ggcttcggta caaagatatg
    11341 cacaaaaatc accaacttct tcatcggtac catttcgacc tataggggtc gaatcagcca
    11401 tgttgctcca tgtgttgcct ctgccaagaa tatccatcct atgagtatct acggcccctg
    11461 ggcatacagc attaacattt atattgtgtg atccaagctc cttcgccatc gattgtgtca
    11521 tacctacaat ggcaaaattc gccccattat aggcaagagt atttgctgat ccacgcttgc
    11581 cagctgtcga tgaaatattt actattttcc cgccgtcacc ctgatcaatc atgggtttga
    11641 tggcggcctt agtacaaagg aatgttccag ttaccttgat atccattacc ttctgaaaag
    11701 tgtcttcatt caggtccaat atcggcgtcc tatcttccgc ccgagcatag gccgcgttat
    11761 ttatcaaaat gtccacccgt ccgaactctt tcatggtttc atccaccatt ctcttaacat
    11821 catccctgtt tgtgacatct accaccaaag gaagcgcccg tctgcctata tctcgaaccc
    11881 gctcagcaac agtttcaatg tctttccaac caattgcctt ttcatcgtca gggaatgaat
    11941 ccgggcttcg tccagttcct gtaacaacaa catccgcccc caatttcgcc aaagcctcag
    12001 cagcagctct gcctatacct cttaatcggc ctgcacctgt aacgattgct acttttccgt
    12061 cgagttctcc catatcaaaa aagctcccct ttatttaggt atttattggc tattgactag
    12121 caagtaaact tgagtatatc ccaaccacac gcgccatgct attctggtac ttcaccagga
    12181 aggtataggt gattcacaac ccacgatttg accttctcaa tgacttcctc agaagattct
    12241 gttaacccgt gacctgtagc cggcataaag atcatttctt tcggttcctc cgcccaatcg
    12301 aagatagtct gggagcactg aggggacaaa acgatatcgt cttcgccgtg aattagtaag
    12361 atcggacgtg gtgatacttc cgaaacatca gttgctccaa acgtttggct tgagagggca
    12421 atgactcctt ttaccttgtc agaaaaaggc gccgatttta tagcaaccgc ccccccaaat
    12481 gaatgtcctg ccagtaaaat gtccgaatga cctgtccctg tcaaaaatga tactccagcc
    12541 aaggtgtcca tgacacattc aatcaactcg ccaggttccc tatagtcaat tcgtaaagat
    12601 gtgattccag gtgatagttc ctcacctaca attcgataca gccccccagc tgggccatcc
    12661 aatcctcccc gagcacctcc tacccatata acaccacggg tggctggttt gtcaggatca
    12721 tgatgaacta tcgtattaat tgggcctcta gtagtttgta aagttaatcc tcttcctgtt
    12781 tgattttcag gaatttccca ttctgcgaga ccgataattc caatcgatat atcatcagaa
    12841 tcttgcattt ttagcccttc taaatagagg ggaattgatt gtgccactcg gtggcttgtt
    12901 cgtatgcata tgccgctttg aacaaattag tctcttgtag ggtgcccgcc ataaattgaa
    12961 gccctaccgg caacccatta accattccgg ccggaattga aattcctggg attccagcta
    13021 tgtttgctgg gattgtgaat atgtcattca aatacatttg taacggatcg tcgattttat
    13081 ctccgatctt aaaggctgta gtaggtgaag tcggcatggc taagacatca acgtctttaa
    13141 acacatcctc aaactcttgt cttatcaaag ttctcacctt ttgggccttc aagtagaaag
    13201 catcataata tccagcggat aaagcatatg ccccgagcat aatccgcctc ttaacttcag
    13261 ggccgaaacc attagacctc gtcttctcta gccctgccca catcgtttca gcatctttct
    13321 cagaaaaccc gtatttaact ccatcatatc ttgcgagatt ggcagaagcc tctgagggtg
    13381 cgattatgta gtagactgct agcgcatact tggtgtgggg aagagaaact tccttcacat
    13441 cagctcccag gcccctcaac acctctaccg aatcaagaat cgatttttca acccccgggc
    13501 tgattccttc aataaaatat tctttcggaa taccgatctt aaaaccctta aggtctgtag
    13561 tcagattact gaaataatct ggtattttta catctaggga agtagagtct ctagagtcat
    13621 gtcctgcgat tgactggaga accaatgcac tatcttccac gctcttagtc aaagggccta
    13681 tctggtccaa tgaactagca aaagccacta atccaattct gcttaccaac ccataagtgg
    13741 gtttcatgcc aacaacgcca cataaagagg ccggctgcct tatactccca ccagtgtctg
    13801 atccaagtga aaatacacat tcggaagctg ctacagcagc agcaggccca ccactgctac
    13861 ctcctggaac cctgttagta tcccaggggt tttttgtcg
//
LOCUS       DASCNS010000259         2042 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001947770, whole genome shotgun sequence.
ACCESSION   DASCNS010000259 DASCNS010000000
VERSION     DASCNS010000259.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001947770"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /gene="mutL"
                     /locus_tag="QF838_09340"
     CDS             complement(<1..1423)
                     /gene="mutL"
                     /locus_tag="QF838_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HJO60920.1"
                     /translation="MPVIRKLTPEISALIAAGEVIERPASVVKELVENSLDAGADRIY
                     ISTSGGGIDSIVVSDNGAGINAEEVEVAFERHSTSKIYKESDLDHISTLGFRGEALYS
                     ISSVSRMELVTRPRGQIEGVRIGLNNHVLTSKSFAGIDYGTTISVSELFSQFPARKKF
                     FKSESAEGSRIHSMIQKFAICNPGVAFELNQGSSRSFRTSGSGVLRDVMVAIFGISEA
                     SKMIEIAEEYDQDWQGPMVSGIIGTPDQKRSNRSRIFLFVNGRLVQSRTLSFAFEQAY
                     KGFSPDNKFPIGAITLQVPLDEVDVNVHPAKTEVRFKNESQVFSVVQGGIKSTLLTQS
                     PVQAIANYTRKDSKFTESIEPSAFWPTALSTGGFKSLEPLSSNTIVGQNFISPYQEVL
                     PLLRVLGQVSDTYIVCEGPDGIYMIDQHAAHERIIFEKIVADAKKETVETQMLLEPIN
                     LELDSKQEILVEENKDILNAFGMT"
     gene            complement(1432..1860)
                     /locus_tag="QF838_09345"
     CDS             complement(1432..1860)
                     /locus_tag="QF838_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60921.1"
                     /translation="MNIYFDVDYTLIAMDGSLRPGTTEVFEELINDGHSIYIWSGVGM
                     RTDDIERLGLSKFVSDIFVKPITEYEVGLRRFSVSPRPDFVIDDHREIVEAFGGVHIE
                     PYYFRSAEDGNMAELYKAIKDFISTGTSNHRGFKPVSEEK"
BASE COUNT          595 a          441 c          359 g          647 t
ORIGIN      
        1 gagtcattcc aaaggcatta agaatatctt tattttcctc aactaatatc tcttgcttcg
       61 aatctaattc cagattgatc ggctcaagca acatttgtgt ttcaactgtt tcttttttag
      121 catctgcaac aattttttca aaaattatcc tctcgtgtgc agcatgttga tcaatcatgt
      181 aaataccatc tggtccctca cacacaatgt atgtatcaga tacttgtccc aagacacgga
      241 gcaatggaag cacttcttgg tatggactga taaaattttg acctacaatg gtgttggagc
      301 taagaggttc cagtgattta aaaccacctg tactaagcgc tgttggccaa aatgcagaag
      361 gctctatact ttccgtaaat ttagaatctt ttcgagtata atttgctatg gcctgcacag
      421 gagattgtgt taaaagagtg ctcttaatcc ctccttggac aacggaaaat acttgacttt
      481 catttttgaa acgcacttca gttttagcag ggtgaacatt aacatccact tcatccaaag
      541 gtacctgaag tgtgatcgcc ccaataggaa atttattgtc aggagaaaat cctttatatg
      601 cttgttcaaa tgcaaaagaa agggttctac tctgtactaa ccgcccatta acaaatagga
      661 aaatgcgaga tctattagaa cgtttttggt ctggagtgcc tatgatcccc gataccatag
      721 gtccttgcca atcttgatcg tactcctctg ctatttcaat cattttggag gcctctgata
      781 taccaaagat agcaaccata acgtctctca aaactccgga acctgatgtc cggaatgatc
      841 tagatgaacc ctgatttaat tcaaatgcca ccccaggatt acatatggca aacttttgta
      901 tcatggaatg gattcggctt ccctcagcag attcggattt gaagaatttt tttcgggccg
      961 gaaattgaga gaaaagttca gaaactgaaa tcgttgtacc gtaatcgatg cctgcaaatg
     1021 atttggaggt gagaacatgg ttattcaaac ctattcgcac accttctatt tgacctctcg
     1081 gtctggttac aagttccatc cgagacactg aagatatact gtaaagcgcc tctcccctaa
     1141 aacctaaagt agatatatga tccaaatctg attctttata tatcttactc gttgaatgtc
     1201 tttcaaaagc aacttctact tcttcagcat tgatgccggc accattatca gatacaacaa
     1261 ttgaatcgat gccaccaccg gatgtggaaa tatatatcct gtctgcacca gcatctaagg
     1321 aattttccac taactctttc actactgaag cagggcgttc aatcacttct ccggctgcta
     1381 ttaaggcaga aatttcaggg gtaagtttcc ttataacagg catagataat ttcacttttc
     1441 ttctgaaact ggtttgaatc ccctgtgatt tgaagtaccg gttgatatga agtcttttat
     1501 cgccttgtac agttctgcca tatttccgtc ttctgcagat ctaaaatagt aaggctctat
     1561 gtgtactccc ccgaaagctt ctacgatctc tctatgatca tcaatcacga aatcaggtct
     1621 tggcgatact gaaaatcttc ttaatccaac ttcatattcc gtaataggct ttacgaaaat
     1681 atctgaaaca aattttgaaa gtcccaatct ttctatgtca tctgtccgca tgccaactcc
     1741 agaccaaatg tatatcgaat gaccatcatt aatcaattct tcaaacactt ccgtagtgcc
     1801 cggtctgagg cttccatcca tagctattag agtgtaatcg acatcaaaat atatattcat
     1861 gaatactaat aggtcctatc taatttatta atatattgca atcatatttt gggaaaatat
     1921 agatttcgcg atctttttta tcgggttttc tttaagttgt atattggctg ccaaagtatt
     1981 gatgatatct gactgcgcca gaagtagtgg agacaccccg cgcctaaggt cactgacttt
     2041 tg
//
LOCUS       DASCNS010000260         5124 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001954323, whole genome shotgun sequence.
ACCESSION   DASCNS010000260 DASCNS010000000
VERSION     DASCNS010000260.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001954323"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="QF838_09350"
     CDS             complement(<1..176)
                     /locus_tag="QF838_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HJO60922.1"
                     /translation="MTTIGIVGCGTIGQALLKSSEAGSLTLPITGIASRTEESARAFL
                     ATLNDPPEYCTQPEL"
     gene            524..1447
                     /locus_tag="QF838_09355"
     CDS             524..1447
                     /locus_tag="QF838_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60923.1"
                     /translation="MTKPLMDFGYNPPCGERGYEVITPRNYLYDLHRALDVASQNFSS
                     IWSSDHLNYGAEFRVECWTMLTWMAARYPGPMLGTVVMCNSFRNPSLLAKMSASLQEM
                     SMGRLILGYGAGWYEEEYRAYGFDYPSAGVRVEMFEEGVQIIKKMWVDSPATFNGKYY
                     HIDNVYCEPRPQPQPILMLGGGGEKKTLKVVALYADWWNDVMRPIPDLSRKLGILRQH
                     CETVGRDYNSIRKTLAPRFFIDKNHKKALRMAEGKNDSGQVIAGDPIAVCEQLHEIAE
                     MGFDMVVTTFPKFQELDDMKLFVDEVIPQFC"
     gene            1496..2257
                     /locus_tag="QF838_09360"
     CDS             1496..2257
                     /locus_tag="QF838_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016228.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60924.1"
                     /translation="MPNPKVLITGASGLIGGLVLDHLYQKYDFSALNRRKVEGVPHRQ
                     ADIASLDEILPAFDGIDTVLHLSAYLEDVNDWDNTNKVNITGTYNVFEAARRNNVSRV
                     VFASSGSTMLGYELDYPYGHIARGEHEKIPDNWKLVDYKDPVRPDSLYGVSKIFGETL
                     GRYFSDYHDISVINIRLGAVLDTDKPKLVRHYPGYLSQLDCVQIIDLCLSAPDSITFE
                     TFDAISDNKLKWRDTSHATKKLGWNPIGRSDQYEL"
     gene            2640..3572
                     /locus_tag="QF838_09365"
     CDS             2640..3572
                     /locus_tag="QF838_09365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HJO60925.1"
                     /translation="MKFGVALTTAASPVFTESEQKDFLLEASEISESKGYESVWVSDR
                     TLYPDDIAKRYPQQFGPGKTDPDSQNIMEALITLSFIAGKTKKIHLGTSVLVLPFRNP
                     LLNTKMISTLDVLSDERLILGIGTGWMKEEFDSMGADYSKRGLVTDEHLSFLSKCSSN
                     SSPEYQGKYINTSGMRLYPQTLNSRSIPIWVGGNSNRAFIRTVKYGAAWHGINLTPPE
                     IIRCQERLKRICESHQKDYSEIGITLRATVRITDKLKHSCGERPYLTGSLGQIETDLQ
                     EYKKSGLDYLIISMAGDTTEQVMTSISRFADSFL"
     gene            3618..4400
                     /locus_tag="QF838_09370"
     CDS             3618..4400
                     /locus_tag="QF838_09370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60926.1"
                     /translation="MSDRLKNVKAVITGAAGAIGMATAQAFLKEGALVFVTDYDTETV
                     DQALKTLKAESDSVFGYPADVTSRDEVAVMMNKAVASLNGITALINNAGIVTPGYLID
                     QLSTDIDRVIDVNTKGVIICTQAAIPYLERSENASITSTSSQAGKRGWDQNAVYSASK
                     AAVIGFSRSMAVELAPGVRVNSIAPGHIKNEGMAWAAFAKRKQRDQTIEDFSADLAAT
                     EIPLKRLQSASDIANGFVFLTSKEASEITGAVLNIGGGVVMD"
     gene            complement(4480..>5124)
                     /locus_tag="QF838_09375"
     CDS             complement(4480..>5124)
                     /locus_tag="QF838_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HJO60927.1"
                     /translation="MHAREIWKVERIATIDWDVHHGNGTQQAFYDDPNVLTISIHQDG
                     LFPADSGKVSERGSKEGENFNINIPLPAGSGHGAYSAAIERIVTPAVKFFQPQLILIP
                     CGFDASVYDPLGRMLLTSESYRELTKMLLALANDTCDGKIVFSHEGGYSKRYVPFCGL
                     ATIEALSGIRTEITDLGGRDDLPGQELVSHQEVLIDEIAQYVKHSILHNSGRLA"
BASE COUNT         1496 a         1065 c         1175 g         1388 t
ORIGIN      
        1 agttcaggct gtgtacagta ttcgggagga tcattgaggg tagcaagaaa ggcacgggca
       61 gattcctctg tcctactagc gatgcccgtt attgggagtg taagggaccc ggcttctgaa
      121 gatttgagaa gtgcctgacc tatcgtccca catcctacga ttccaattgt cgtcactgat
      181 ctagatcatc ctccttcaag catttaagta tcgcaacttc cacagtagca cgtatcctcc
      241 agatatgctt ctcttagctg aagccaatct tacaaaatgc agtccgacat taatcgtgtg
      301 catgggaaaa atttaccatc ctacttaaag tgcaatgcgc tattcgaaca atggctatgg
      361 aataaaacgc cgagagatta gtctttagcg gttccagcta attccactca ctttgggatc
      421 cgttattaca gacccattta ttgctgtcct ctgcaagtgg ctgaaataaa ggagtagggt
      481 atatttacca tgaacaaaca aaagtttagt gaggaggaat actatgacaa agcccttaat
      541 ggattttggc tacaacccac cttgtgggga gcgcggttat gaagtgatca caccaagaaa
      601 ctatttgtat gaccttcata gggcacttga tgtcgcctct caaaacttta gttctatatg
      661 gtcgtcggat catttgaatt atggggcaga attcagagta gaatgctgga cgatgcttac
      721 ctggatggca gccagatatc caggacctat gttgggcaca gtagtaatgt gcaattcttt
      781 ccgaaacccg tctctcttgg ctaaaatgtc tgccagcctg caagaaatga gcatggggcg
      841 tctaatactc ggttacggtg ctggttggta tgaagaggaa tatagggcat atgggtttga
      901 ctatccttct gctggtgtaa gggtggaaat gtttgaagaa ggtgtacaaa tcataaagaa
      961 gatgtgggta gacagtcccg ccaccttcaa tggtaagtac tatcatattg ataatgttta
     1021 ctgtgaacca cggccacagc cacagccaat tttaatgttg ggggggggtg gtgagaagaa
     1081 aacattgaag gtagtagccc tgtatgccga ttggtggaat gatgtaatga ggcccattcc
     1141 cgatctatca cgcaaactag gtatactccg acaacattgt gaaaccgttg gacgagacta
     1201 taacagcata cgaaaaactc tggcccccag gtttttcatc gataaaaatc acaaaaaggc
     1261 actgaggatg gctgagggca agaatgactc cggccaggta atcgctggtg acccaattgc
     1321 agtgtgcgaa cagttacacg aaatagcgga aatgggattt gacatggtag tgactacttt
     1381 tcctaaattt caggaactcg atgatatgaa actatttgta gacgaggtaa ttccccaatt
     1441 ttgctagccg ggatagcctg ctaagaaact gaatcgttac acaggagact caaatatgcc
     1501 caaccccaaa gttcttatca caggcgcaag cggtttaatt ggtggattgg tactcgatca
     1561 cttgtaccaa aaatacgatt ttagtgcttt gaatcgccgg aaagttgaag gggtacctca
     1621 tagacaggcg gatatagcta gccttgacga aattcttcct gcgttcgacg gaatcgacac
     1681 agtattacat ttatctgcct acttggagga tgttaacgat tgggataata ccaataaagt
     1741 aaacataacg ggtacgtaca atgttttcga agctgcaagg agaaacaatg tatcccgggt
     1801 cgtttttgca agttctggaa gtactatgct tgggtatgaa ctggattacc catacggcca
     1861 tatcgcacgc ggggagcatg aaaaaatacc tgacaactgg aaacttgtag attacaaaga
     1921 tccagtgaga cctgatagtt tgtacggtgt gagtaaaatc tttggggaga ccttgggaag
     1981 atacttctca gactaccatg acatctctgt tataaatata agactcggtg cggtcttaga
     2041 tactgataaa cccaaactag tccgccacta cccagggtat ttaagtcaat tagattgtgt
     2101 ccaaatcatc gatttatgtt tgagtgctcc agattcaatt acatttgaga cttttgatgc
     2161 gatttcagac aacaagctga aatggagaga cacatcccat gccacaaaaa aacttggttg
     2221 gaaccctata ggtagatccg accaatatga attatgaaaa ccttcaaaat gatgctaatc
     2281 accaccgggc gtaaggtaaa tcaggataga taactcgtgc aggttgtgtg gacaatggct
     2341 aataccgtta cttcaactgg tacctcaatt ttcttgatga tttcttggat acctatctga
     2401 gaggtgtgtg tgactacact tcctgcataa ttccacgtta agacgggaca tacatagcct
     2461 gtaattcaaa gagtaccctt ccgaccggta accacacaca gacagcatgc tcgaaaagtt
     2521 aatctgcttt ttgtattaga cctgagttca ccactattga gataattcac atattcaaat
     2581 gtgtacaatt cgcactttag ctactaatgt tcatcagatt ggagaatggg agttagaaaa
     2641 tgaagttcgg tgtcgcctta actaccgctg cgagccctgt cttcacagaa tccgagcaaa
     2701 aagatttttt gcttgaggct tcagaaattt ctgaatctaa gggttatgaa tccgtttggg
     2761 ttagtgatcg tacgctatat cctgatgaca ttgccaaacg atatccacaa caatttgggc
     2821 ctggcaagac ggatcccgat tctcaaaata ttatggaagc tcttataacc ttatctttca
     2881 ttgccggcaa aaccaaaaaa atacacctgg gaactagtgt tctagtctta cctttcagga
     2941 accctttgct aaataccaaa atgatttcaa ccctagatgt tctttcagac gaaagattga
     3001 tcctgggtat tggtactgga tggatgaagg aagaatttga ttcgatgggg gcagattatt
     3061 cgaaaagagg attagtgact gatgaacacc tgagtttcct gtcaaagtgc agtagcaatt
     3121 cttctcctga atatcaagga aaatacatca atacctctgg catgagactt tatcctcaga
     3181 cactcaatag tcgatcaatt ccaatttggg taggaggaaa ttcgaacaga gcttttatta
     3241 gaactgtgaa atatggtgca gcctggcatg ggattaacct aaccccgccg gaaataatac
     3301 gttgccagga aaggttaaag cgaatttgcg aatcccatca gaaagactat tctgagatag
     3361 gaataaccct cagggctacc gtgagaataa cagataaatt aaaacattct tgcggtgaaa
     3421 gaccttatct aactggtagt ttgggtcaaa tagaaaccga tctccaagaa tacaagaaat
     3481 ccggtttaga ttatcttatt atttcaatgg caggagatac cactgagcaa gtaatgacat
     3541 caatatcaag atttgcagat agcttcttat aaacaccatc ttcacgtgaa ggactaaaat
     3601 tattggagga attagcaatg tctgacagac tgaaaaatgt gaaggctgta atcaccgggg
     3661 ctgctggggc catcggtatg gccactgccc aggcgtttct aaaagaaggc gctctagtgt
     3721 ttgtaactga ttatgatacc gagactgtag accaagccct aaaaacactt aaggctgagt
     3781 cagattctgt gttcggttac ccggcagatg taacttcaag ggatgaagtt gccgtcatga
     3841 tgaataaagc ggtggcttca ttgaatggca tcaccgctct gatcaataat gcaggaatag
     3901 tcacaccagg ttacctcatt gaccaactat ccacggatat cgaccgggta atcgatgtga
     3961 acacaaaggg tgtgattatt tgcactcagg cagctatacc ttatctagaa agatctgaaa
     4021 acgcgtcgat tactagtact tcttcccagg cgggtaaaag aggttgggat cagaatgctg
     4081 tctacagtgc ttcaaaggcc gctgtaatcg ggtttagccg ctctatggct gtggaattgg
     4141 cccctggcgt cagagtaaat tcaattgcac ctgggcatat caaaaacgag ggcatggctt
     4201 gggcagcctt cgctaaacgc aagcaaagag atcagaccat cgaggatttt agcgctgatt
     4261 tagctgctac ggaaatccct cttaaacgtc tccagtccgc gtctgatatc gcaaatgggt
     4321 ttgtatttct aacctcaaaa gaagcgtcag aaatcaccgg tgcagtgttg aatattggtg
     4381 gcggggtagt tatggattaa cagtcctcct tcggcagaaa cggaccgctt ggtttaggaa
     4441 tcttgttatg gggtggcttg gcctatttct tgtcgatcgc tacgctagtc gtccagagtt
     4501 atgcaaaatt gagtgtttta catattgagc aatctcatca ataagaactt cttgatgaga
     4561 taccaattcc tgtccgggta aatcgtcacg gcctccaaga tcagttattt cagtacgtat
     4621 cccgctgagt gcttcaatcg tggcaaggcc acaaaatggc acatatctct ttgaataccc
     4681 accttcgtga gagaacacaa ttttgccgtc acaggtatcg tttgcaagag ccagaagcat
     4741 tttggtaagt tctcgatacg attcagatgt cagtaacatt cgtcccaatg gatcataaac
     4801 gctggcgtcg aatccacagg gtataagtat taactgaggt tgaaaaaact tcacggcagg
     4861 tgtcacgatc cgttctatcg ctgctgagta ggcaccatgt ccagagcctg cgggtagagg
     4921 aatgttgata ttgaagtttt ctccttcctt acttcctcgc tcactaacct taccggagtc
     4981 tgccggaaac agcccgtcct gatgaatgga gattgtcagt acgtttggat catcgtaaaa
     5041 cgcctgctgg gttccgttac cgtgatgaac gtcccaatct atagttgcta tccgttcgac
     5101 cttccaaatt tcgcgggcat gcat
//
LOCUS       DASCNS010000261         7608 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001956818, whole genome shotgun sequence.
ACCESSION   DASCNS010000261 DASCNS010000000
VERSION     DASCNS010000261.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001956818"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="QF838_09380"
     CDS             complement(<1..999)
                     /locus_tag="QF838_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HJO60928.1"
                     /translation="MTTYEPNMVLAEKEFSVVGTRPIRHDGTDKVTGRARYSADSFPA
                     GYLHGKILRSPHAHARIKSIDTSKALAYPGVKAVMTGADLPEVSAEVADLEEGAAVNY
                     GFYSRNVMAREKALYVGHAIAAVAATDSHIAEEALALIEVDYEVLTPVLTAEDALKED
                     APVLHDRLLTQSSVLFRVGGWGDESTAGLTNLALKIISQEGDVDKAFAESDVVVEREF
                     KTKAVHQGYIEPQASTAFWDENGKVTAWCSTQQLFAFRDHISAMLDIPVGNVSVVPLE
                     IGGGFGGKGQGGVYLEPVAAVLSKNSGSAVKITMTRAEVFMGTGPTSGTAMEIKLGA"
     gene            1190..2134
                     /locus_tag="QF838_09385"
     CDS             1190..2134
                     /locus_tag="QF838_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60929.1"
                     /translation="MKFGASISSYTTTWDSIETAIKTMEIGNWDSIWFPDHFIPPAAW
                     KGAEDQPIFESWSLLAAVAGMTKKLRMGHMVNGNTYRNPALVAKMATTIDQISRGRFI
                     LSHGAAWFQREHEAYGWEFPSLRERSDRFEESCALIRILLSSEEPVNFKGNYYSLENA
                     PLSPRSVQKPHIPIMVGGLGEKRTLRTLAKYGDMWNLDGFGVGKEENVRYGGMSLELF
                     NRKVNILHKHCEDVGRDPSEISLTISMPTKLSNDQKETDAFIKQVGPGTVAGSADYIV
                     SRVGEFMEAGVDEIMFSPRPSDSESLQALDEEVLSAFA"
     gene            complement(2131..3084)
                     /locus_tag="QF838_09390"
     CDS             complement(2131..3084)
                     /locus_tag="QF838_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HJO60930.1"
                     /translation="MGNILVTGAAGQIGSELVPALRENYNVDTVIAGWNKTPLPEDIL
                     ETGPNVVLDVTDFDALERIVRDFKIGTIFHMSSILSATAETIRTTAHDVNVNGTFNIL
                     ECAYLNGLEQVVLPSSIAAFGDKTPRSKTPNDTIQNPDTVYGISKVFGELMGNYYHSK
                     RGLDVRGIRLPGIISWKTEPTAGTTDYAVAMFYEAIRKNSYECYLASDTALPMMYMPD
                     AINALIKLYQADSQALIHHSDFNIHAMTFTPDDLAREIIKINPRFEIEYKIDPVRQSM
                     ADSWPISLDDSKAREEWGWEPEYSIQDLAEDMYLNLSKKLS"
     gene            complement(3105..3857)
                     /locus_tag="QF838_09395"
     CDS             complement(3105..3857)
                     /locus_tag="QF838_09395"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60931.1"
                     /translation="MGKLEGKVAIVTGAGQGMGSAIAHSLAQQGASVVVNDMNETTAE
                     ATVSDIKSYNPKVISYVGNVTDICDVQQMIEHTESHFGHIDVLVNNAGVLRPTPLLQI
                     SEEEWDYVVEGNLKSTFLCSKAVLPSMQQNRWGRIINISSSAGKNVSTIGGAHYTAGK
                     AAVLGLTRHAAKEVAEYGITVNAVCPGLIETEMVRSTISDERKHEYADMFPVKRLGQP
                     SEVADLVSFLASDQASYITGASIDINGGDLMI"
     gene            complement(3962..5539)
                     /locus_tag="QF838_09400"
     CDS             complement(3962..5539)
                     /locus_tag="QF838_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HJO60932.1"
                     /translation="MEYDYIVVGAGSAGAIIAARLSEDESKSVLLLESGSDYGDFSSL
                     PEKFKYGYGPELLDEPNWWLDPGDEKRWLFVANATEEQDSPMLIPRGKAMGGSSAVNA
                     QIFLRGDPQDYDTWAEHGNEEWSFEKCLPAFKKLENDTDMGGDFHGHDGPVRVRRHPK
                     DEWTRDAEAFFQGFIDLGFPATDDHNDPDSTGVGPMPFNTINRVRQSTALTYLNPARN
                     RTNLTLRADCNVRRIKLSNKKAVGVEVEKGGDIFVERGSEIIVSAGAIGSPQLLMLSG
                     IGPNQHLEGKGINVRHHLPGLGQNLRDHPQVRLIWEVKDDYEHLKDGSKRGATVAIRY
                     TAEGSELHNDMMVHHTATVPQRFYMTDGEDMYKGVGMSACLYLQIGSGELLLRSSDPQ
                     VQPHLDYNYFREEEDLRRMRECVRLCAEVGDGPSYESIVAGRVQPTDQELEDNDLLDL
                     WILQNATTSHHISGTCKMGPESDPMAVVNQYGKVHGFENLRLGDASIMHDCVRANTNV
                     PAMMIGEKIAEFIKEGK"
     gene            complement(5604..6410)
                     /locus_tag="QF838_09405"
     CDS             complement(5604..6410)
                     /locus_tag="QF838_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60933.1"
                     /translation="MIDLSNKNALVTGAASGIGKGISKVLAEQGANVVLSDIDPNGAR
                     LASEEISGSGGKSQSVHLDVTNRASAQSVVLDLLDQLGQIDIVVNNAGVGGARGWWER
                     KVPSEDDWDAVLAINLRGVVNISQAVEDGMKERRQGKIVNIASIASRQGGPSIPHYNA
                     SKAGVVSWTQSHALQLAPYQINVNAICPGLLWTPLFEQLIGRPDFYDNQALEGLTGRE
                     RFEKVIELGIPMQKEQTPEDIGKLTAFLASDDAHNITGQAINVDGGRRMN"
     gene            complement(6437..>7608)
                     /locus_tag="QF838_09410"
     CDS             complement(6437..>7608)
                     /locus_tag="QF838_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HJO60934.1"
                     /translation="TKGVKTTGGSLAYKDRVPDADCAVVERVLSAGAIMLGKTNSPEF
                     GLLGANENRLGDPCRNPWNPERTSGGSSGGAGASVIAGLTSLATGGDGGGSIRIPASF
                     TGTYGIKPTQGRVSSFSGINAPAAANYTSQQGGLTRTVRDSALMLQVLSGYDPRDPGS
                     LRAPVPDFMAATERDVSGLRIGWSPDFGYTSVDTEVAEITKNAIEAFSELGCSIDQSG
                     LKLENPFDPWLVIFAAAAYVTNGHLLDDPADPLTWYGKWAIEQGRDVTTTDYVRALGS
                     RNQMIHLFEDEFEKFDVLVSPTMPTTAFPTDIYPQKIGDQDPYPNPGWGFVPFTHPIN
                     TIGFTAASIPCGFDSDGMPVGLHIVGKHGDEETVLAVSAAFESARPWIHRRPPVS"
BASE COUNT         2045 a         1944 c         1510 g         2109 t
ORIGIN      
        1 agctcccagt tttatttcca tggcggtacc agatgtcggt cctgttccca tgaaaacttc
       61 ggctcgagtc attgttattt ttacagcaga acctgaattt ttagacaaaa ccgcagcaac
      121 aggctccaaa taaactccgc cctgcccttt gcctccaaag ccaccaccaa tctccaaagg
      181 caccacactg acatttccca caggtatatc tagcatcgca gatatatgat ctctgaaagc
      241 aaatagctgt tgcgtactgc accaggcagt caccttccca ttttcatccc aaaaagcagt
      301 gcttgcttgg ggttctatat agccttggtg aactgccttg gttttaaatt cacgctcaac
      361 gaccacatca gattcagcaa atgccttatc gacatctccc tcttgcgaaa ttatttttaa
      421 ggccaggtta gtcaaacctg cagtactctc atcaccccat cctcctaccc tgaacaagac
      481 acttgactga gtcaataacc tatcatgtaa gacaggtgcg tcctctttaa gagcatcctc
      541 agcggtcaat actggagtca aaacctcgta atcaacttcg atcagagcca aagcttcctc
      601 ggcaatatgg gagtcagtag cggccactgc ggctattgca tggccgacat agagagcttt
      661 ctcccgggcc attacattcc ggctatagaa accataattt acagcagcac cttcttccag
      721 atcagctact tctgccgaaa cttctggcaa atcagctcct gtcataacag ccttaacccc
      781 gggataagca agagctttag aagtatctat agattttatc cttgcatggg cgtgggggct
      841 tctaagaatt tttccatgca gataccctgc agggaatgaa tcagcactat acctagcacg
      901 gccggttact ttgtcagtac catcatggcg tatcggtcta gtacctacta cagaaaattc
      961 cttttcagca agtaccatgt ttggctcata ggtggtcatg gtgcatctct ccattaatct
     1021 gatttgttaa tctaagttaa tttaacacat cgaaaagaaa acagacgtaa attaaaatta
     1081 agtcagtaaa atggtacgca gtatgtctat tacccaggaa gttatgtgaa gtaaattagt
     1141 aggtataaga tgtaactatg aaacagcaaa cgattataag gatgactaaa tgaaatttgg
     1201 agcatcaata tcaagttaca ccaccacatg ggatagcatc gaaacagcga taaaaaccat
     1261 ggagatagga aactgggata gcatatggtt tcctgaccac tttataccgc cagctgcgtg
     1321 gaagggggct gaagatcaac caatttttga atcgtggtct ctactggctg ctgtcgctgg
     1381 aatgaccaaa aagctccgaa tgggtcatat ggtgaacgga aatacctacc gcaatcctgc
     1441 attagttgca aaaatggcaa caaccataga ccagatatcc agaggcagat tcatcctcag
     1501 ccacggtgcg gcatggtttc aaagagagca cgaggcctac ggctgggaat ttccatccct
     1561 gcgggaacgg tcagatagat ttgaggagtc atgcgccctt atcagaatcc ttctcagctc
     1621 cgaggaaccc gtaaatttca aaggaaacta ttacagttta gagaacgcac cgttatcccc
     1681 aagatccgtt caaaagcccc atattccaat aatggtaggg gggttggggg agaaacgcac
     1741 actcagaaca ctagccaaat acggggacat gtggaaccta gatggtttcg gtgtaggtaa
     1801 agaagaaaac gtacgttacg gcggcatgtc gctggagttg tttaaccgaa aagtcaatat
     1861 tctgcataaa cactgtgaag atgttggtag agatccctca gaaatatctc tcacaatttc
     1921 tatgcctacc aaattgtcca acgatcaaaa ggaaacagat gctttcatca aacaagttgg
     1981 acctggaacg gttgctggta gtgctgacta catcgtttca agggtcgggg agttcatgga
     2041 agcgggtgta gatgaaatta tgttttcacc gagaccaagt gacagcgaat ccttacaggc
     2101 acttgacgaa gaggttctct cggcattcgc ttaggaaagt ttttttgaga ggttcaggta
     2161 catatcttcg gctaaatctt gaatagagta ttcaggttcc caaccccact cctccctggc
     2221 tttggaatca tcgagggata taggccatga atctgccatc gactgtctga caggatctat
     2281 tttatattcg atttcaaacc ggggattgat ttttattatc tctctcgcaa gatcgtccgg
     2341 agtaaacgtc atcgcatgga tattgaaatc tgaatggtga atcaaagcct gagaatccgc
     2401 ctggtacaac tttattaaag cattgattgc atctggcata tacatcatag gcagagcagt
     2461 atcggaggca agatagcatt cgtacgaatt tttccttatc gcctcataga acatcgccac
     2521 tgcgtagtct gtagtcccgg ctgtcggttc tgtcttccag ctaatgatcc ctggtaatct
     2581 tatgcccctc acatccaggc cccgctttga atggtaataa ttgcccatca attccccaaa
     2641 aacctttgat atcccataaa ctgtgtcggg gttttgaata gtgtcgttgg gggtcttact
     2701 tcggggagtc ttatctccaa aagcagcaat agaacttggt aataccacct gctccaggcc
     2761 gtttaaatat gcacattcca aaatgttgaa cgttccgttg acattcacat catgcgcagt
     2821 agttcttatg gtttccgcag tggcagacag tattgatgac atatggaaaa ttgtgccaat
     2881 tttaaaatct ctcacaattc tttctaaagc atcgaaatct gttacatcca aaacaacatt
     2941 tgggccggtt tcgagtatgt cttcaggtag aggggtctta ttccaccctg ctatcacagt
     3001 atcaacgttg tagttttctc ttagagcagg aaccaactca gacccgattt gccctgcagc
     3061 accagttaca agaatgttac ccatttcaat ctcattcccc ttttttatat catcaaatct
     3121 ccaccattta tatcgatgga tgcccccgtt atatacgagg cttggtcaga tgccagaaat
     3181 gaaacaagat cagccacttc tgaaggttgt ccaagccgct ttacaggaaa catgtctgca
     3241 tattcgtgtt ttctctcatc tgaaatagtt gatctcacca tttctgtttc gatcaagcca
     3301 ggacacactg cattaaccgt tatcccatac tcagcaacct ctttagccgc atgccttgtt
     3361 aaaccaagga ccgctgcttt tccggccgtg taatgtgctc caccaatcgt gctgacattt
     3421 ttcccagcac tcgatgaaat attgattatc cgcccccatc tattttgctg cattgagggc
     3481 aacacagcct tggagcacaa aaaagttgat ttcaaattcc cttcgaccac gtaatcccat
     3541 tcctcttcag atatctgaag cagaggtgtg ggccgtaata ctccagcgtt attgaccaac
     3601 acatcgatgt gtccaaaatg actttcagta tgttcgatca tttgttgaac atcacaaata
     3661 tctgtgacat taccgacata tgaaatcact ttagggttat agcttttgat atcagagaca
     3721 gtcgcctcag cagtggtctc attcatatcg ttaactacaa ctgaggcgcc ctgctgagcc
     3781 aatgaatggg ctatagcgct acccatacct tgaccggcac cggttactat tgccactttt
     3841 ccttccaatt tacccacagt tagactcctc tagattttac ccggttagtt ttcccaccca
     3901 gaaagtgtac atagtccaat agtgaaccga ttcacctgga ctccaattca cacctaaatg
     3961 atcatttacc ttctttgata aattcagcga ttttttctcc gatcatcatt gccggaacat
     4021 ttgtgtttgc ccttacacag tcgtgcatta tcgatgcatc acctaaacgt aaattttcaa
     4081 aaccgtgaac tttaccatac tggttaacca cagccatcgg atctgattca ggtcccattt
     4141 tgcaggtacc acttatatgg tggctcgttg tagcgttttg gaggatccat aaatctagca
     4201 aatcattatc ctctaactct tgatccgtcg gttgaacacg tcctgcgact attgactcat
     4261 aagaaggtcc atcccccact tctgcgcaca acctcacaca ctctctcatc cgcctcaggt
     4321 cctcctcttc cctaaagtag ttatagtcca gatgaggttg aacttgtgga tctgaagatc
     4381 tcagcagaag ctcccctgag cctatttgaa gatacaggca agcactcata cccacgcctt
     4441 tatacatatc ctcaccgtca gtcatgtaaa acctctgtgg aactgtagcc gtatgatgaa
     4501 ccatcatatc gttgtgaagt tccgatcctt cagcggtgta ccgtattgca accgtcgcac
     4561 cacgttttga accatccttt agatgctcat aatcatcttt tacttcccat ataagccgaa
     4621 cttgggggtg gtctctcagg ttttggccga gaccgggtaa gtgatgtcgc acgtttattc
     4681 cctttccctc gagatgctga tttgggccta taccagacag catgagcaat tgcggtgacc
     4741 ctattgcacc ggcactgaca attatttccg atcctctctc cacaaaaata tccccacctt
     4801 tctccacttc caccccaaca gcctttttat tactcagttt tattcttcgg acgttgcagt
     4861 cagctctcaa agttaggttg gtacggtttc gggcaggatt taggtaagtc aaagctgtac
     4921 tctggcgaac tctgttaata gtgttaaatg gcatcgggcc aacgccggtt gaatcaggat
     4981 cattatgatc atctgtagct ggaaatccca aatctataaa gccctgaaaa aatgcctctg
     5041 cgtctcgcgt ccattcgtct tttggatgcc ggcgaacacg tactggccca tcgtggccat
     5101 gaaaatcccc ccccatatca gtatcgtttt ctaatttttt gaaggctgga aggcactttt
     5161 cgaaactcca ttcttcgttt ccatgctccg cccacgtatc atagtcttgt gggtctcctc
     5221 gaagaaaaat ctgtgcattc acagcactag acccgcccat cgctttaccc ctaggaatca
     5281 acattggaga atcttgttct tctgtagcat tggcaacaaa aagccaacgt ttttcatcac
     5341 cagggtcaag ccaccaattt ggttcgtcta acagctctgg cccataccca tatttaaact
     5401 tttctggaag tgaagaaaaa tccccgtagt cactacctga ctccagaaga agcacagatt
     5461 tagattcatc ttcactcaat cttgcagcta taatggctcc agcagacccc gctccaacaa
     5521 ctatgtaatc gtattccatc tagcagctcc attcttataa tctctctaac caggcaagtg
     5581 tcaatgaaag gaaaactgga gatttaattc atccgccgcc ctccatcaac attaattgct
     5641 tgccctgtaa tattgtgggc atcatcagag gctaaaaagg ccgtcaattt gccaatatct
     5701 tccggcgtct gttccttttg cattggaatc cccagctcta tgactttttc aaatcgttct
     5761 cgacccgtaa gtccttccaa agcttgattg tcataaaaat ctggccggcc gataagctgt
     5821 tcaaataatg gtgtccataa taagcccgga catattgcat ttacattgat ttgatatgga
     5881 gccagctgaa gagcatggga ctgtgtccaa ctcacaactc cagcttttga agcattgtag
     5941 tggggaatag aagggccacc ttgccgggat gcgatcgaag caatattaac tattttccct
     6001 tgcctccttt ctttcatacc atcttcaaca gcctgtgaaa tgtttactac gcctctgaga
     6061 ttgatagcca gtaccgcgtc ccaatcatct tcacttggaa ccttcctctc ccaccagcct
     6121 cttgctccac caaccccggc gttatttacc acaatgtcta tttggcctaa ttgatccaat
     6181 aaatctaaaa ctaccgattg ggcagaagct cggtttgtga catccagatg caccgactgg
     6241 gacttaccac cggatccgga aatttcttct gatgcaagcc tagctccgtt cggatcgata
     6301 tcacttaaca ccacattagc cccttgctca gcaagaacct ttgatatgcc tttaccgatt
     6361 ccagatgcag ctccagtaac taacgcgttc ttattactta aatctatcat tttaaattct
     6421 ccaaaatgcg ttttatctaa cttacaggcg gcctgcgatg aatccatggt cgggccgatt
     6481 cgaaagctgc actaactgca agaacagtct cttcatcccc atgtttgccc acgatatgca
     6541 atcctacagg cattccatca gagtcgaatc cacatggaat actcgctgcg gtaaacccaa
     6601 tggtattaat tggatgagta aaaggtacaa acccccatcc agggttaggg taagggtctt
     6661 ggtcccctat tttttgaggg tatatgtcgg taggaaaagc ggtagtgggc atagtcgggg
     6721 ataccagaac gtcaaatttc tcaaattcat cctcaaaaag atgaatcatt tggttccttg
     6781 atcctagagc cctcacataa tctgttgtcg taacatccct accttgttct attgcccatt
     6841 tcccatacca ggtgagcgga tcagctggat catctagcaa atggccgtta gtcacgtacg
     6901 ccgccgccgc gaatatgaca agccaaggat caaaaggatt ttccagtttc agaccagatt
     6961 gatcaattga acatcccaat tcggaaaaag cttcaatcgc atttttagtt atttctgcta
     7021 cctctgtatc cacggaggtg tatccaaaat ctggactcca gcctatacgt agtcctgata
     7081 catccctttc agttgcggcc ataaaatcag ggacaggggc acgaagagaa ccggggtctc
     7141 tgggatcata tcctgacaac acctggagca ttaaagcaga atctctaacg gtccgggtta
     7201 atcctccttg ctggctggta tagttggcag ccgcaggagc attaatccct gagaaactag
     7261 acactctccc ctgagtaggc ttaattccat aggtgcctgt aaaactagca ggtatccgaa
     7321 ttgagccgcc gccatctcct cccgtggcca aggatgtcaa tcctgcgatt acggatgccc
     7381 cagcacctcc actggatcct cccgacgtac gctcaggatt ccacggattc ctgcagggat
     7441 ctcctaatct attttcattg gctcccaaca gcccaaattc cggggaattg gtttttccca
     7501 acatgattgc cccagcggac agcactcttt cgaccacggc gcaatcagca tcgggtactc
     7561 tgtccttata tgccaatgaa cctccagtcg tcttcacccc tttggtca
//
LOCUS       DASCNS010000262         3557 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001959568, whole genome shotgun sequence.
ACCESSION   DASCNS010000262 DASCNS010000000
VERSION     DASCNS010000262.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001959568"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="QF838_09415"
     CDS             <1..1157
                     /locus_tag="QF838_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJO60935.1"
                     /translation="IENARHRVTVSFDERSKGFVWFFSFLSYFSHLEETESGDLIILL
                     DEPGTALHAMAQKDFLRFMDERLSPRCQVLYTTHSPFMIDLEKLHRIRLVQDMDGVGT
                     VITDDPVHNDRETVFPLQMALGYQMAQTLFLSPHCLMVNSPSDLIYLQVLGDMVAAEA
                     GSRIDPRWVIIPVGSTDNLSTFITLLGDNYVSVAVMMDLTPTNKEKIEAINRKNELEG
                     ENPVKWVQVTRVRDADIEDLFDPKVYLQLVNLSYANQLDHTLTMRSITESNPRIVERL
                     KVYFAKTGIAGGVFDRYVPAAYLLENFDDFKVHISEDTIEKAKTLVERINSLITEVDS
                     IDTLNSSKGRALGGSRTASNKSNINATGTVGIDSIQSSAAVGSLPLPTQF"
     gene            1163..2524
                     /locus_tag="QF838_09420"
     CDS             1163..2524
                     /locus_tag="QF838_09420"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HJO60936.1"
                     /translation="MKEDSDYYRAVEQTLTLADFERGKHSPNHSLFHLERMRLLCTQL
                     GNPQSDIPSVHIAGTKGKGTTSAMITAILDSSGYKVGLVTSPHLHSLTERIRIGSEPI
                     SKNDFTNLVKQIWPLVMETGQNSQYGEVTWFEFMVSAAFHFFGESNVDFQVVETGLGG
                     RLDATNILLPIVSVITSISLDHISILGDSLAQIASEKAGIIKDEIPVVISPQHRESMN
                     VVTGIAAACNAPIKKVSEDYSVAIKHKDLSGQYLQVKTPLRDYEFKLPLLGIHQTENA
                     LTAICAIETIKELGYLIDGDSVETGLSEVCWPGRFEVLKTSGPILVVDGAHNPYSMER
                     LVETVNDHMSNSKVIVVFGATGGHDLANMLEPLKLLEPVLIPVSSRHPKSIQSEIVSK
                     ASLNCDIVTSKAFGSVSKGLDHAFSLAGRNDLILGTGSLSVAAEVSEVIKEISPEIYP
                     NLP"
     gene            2574..>3557
                     /locus_tag="QF838_09425"
     CDS             2574..>3557
                     /locus_tag="QF838_09425"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HJO60937.1"
                     /translation="MAKDNTRVVITGLGVMSPLGQTVEEFWDGLSNGRSGIGRITLCD
                     TEGFPSDIAGEVTGFDAGQYADPKEVRRMARFSQFAVAAAATAIEDSALDLSSNNDRV
                     GVVMGNGNGGFPTTEDNAKILFEKGGMKVSPFFIPMILPNMAAAQVSRVFNLKGYSST
                     LITACAAGNQAIGEAYEAIRRGAADVIVAGGCEAGISRIGLGGFHVIRALSRFDGDPQ
                     GASKPFDSNRDGFVPAEGAGILIMETLDHAQKRGADIKGEIVGYAVSSDAYHAVQPDK
                     NGEGAARAITWAISNADKRLEDIDYINAHGTSTPLNDASETKAIKLAFGDLA"
BASE COUNT         1030 a          676 c          857 g          994 t
ORIGIN      
        1 gaatagaaaa tgcgaggcac cgcgtcacgg tgtcatttga tgaaaggtcc aaagggtttg
       61 tctggttttt ttcttttttg tcgtattttt cgcatctaga agaaacagaa agtggtgatt
      121 tgataatcct acttgacgag ccaggtacag cattacacgc catggcccaa aaagattttt
      181 tgagattcat ggatgaaaga ctttcaccaa gatgtcaggt tctatacacc acccattcac
      241 cttttatgat tgatctggaa aaactgcatc gaatcaggtt ggttcaggat atggatggtg
      301 taggcactgt gataaccgat gatcctgttc ataatgacag ggagactgta ttccctttac
      361 agatggcctt gggttatcag atggctcaaa ccttatttct atctccccac tgtttgatgg
      421 ttaattctcc gtctgatttg atatacctac aagttttggg tgatatggtg gcagcagagg
      481 ccgggtcgag gatcgaccca agatgggtga ttatacccgt gggtagcacg gataaccttt
      541 ctactttcat cactcttctt ggggataact atgttagtgt cgcggtgatg atggatttaa
      601 ccccaacaaa taaggaaaag atagaggcta tcaataggaa aaatgaatta gaaggcgaga
      661 accccgtaaa gtgggttcaa gttaccaggg tacgagacgc ggacatcgag gatttgtttg
      721 atccaaaggt ttatttacaa ttggtcaacc tttcttatgc taatcaactg gaccatacat
      781 tgactatgag atccatcact gaaagtaatc caaggatcgt ggaaagactg aaggtatatt
      841 tcgcgaaaac aggaatcgct gggggtgtct ttgatcgata tgtgcctgcg gcatatcttc
      901 tggaaaattt tgatgatttc aaagtgcaca tatctgaaga tactatagaa aaagccaaaa
      961 cgttggtaga aagaataaat tctttgatca ctgaagtaga ttcaatcgat actttgaatt
     1021 cctctaaagg tcgtgcctta ggcggatctc ggactgcatc taacaaatca aatataaatg
     1081 cgactggtac ggtaggaata gattctatac agtcatcggc agcagtaggg tcactacctc
     1141 tcccgactca attctagcgc ccttgaaaga agatagtgat tactaccgtg ctgttgagca
     1201 gactcttact ttagccgatt tcgaaagagg caaacatagt cctaatcatt cgctctttca
     1261 cttggaaagg atgcgtctcc tctgcactca actgggcaat ccccaatcgg atattccctc
     1321 tgttcatata gctggcacaa aaggcaaagg taccacctca gccatgatca cggctatatt
     1381 ggattcatct ggatataaag tgggcttagt aacttcacct catctacatt ctttaactga
     1441 gaggattcgg atagggtctg agccgatttc caaaaatgat tttactaatc ttgttaagca
     1501 gatctggccg ttagttatgg agactgggca aaatagtcaa tatggtgagg ttacttggtt
     1561 tgagtttatg gtatcggccg cgttccattt ttttggtgaa tcaaatgttg atttccaggt
     1621 tgttgaaact ggattaggcg ggaggttgga cgcaacaaac atactattac ctattgtttc
     1681 agtaatcact tctatcagtc ttgaccatat cagcatatta ggcgatagct tagcccaaat
     1741 tgcctctgag aaagctggaa tcataaagga tgaaatccca gtagtcatat ctccgcagca
     1801 cagggaatcg atgaatgtcg tgacaggaat tgctgctgca tgtaatgcac cgatcaaaaa
     1861 agtcagcgaa gattattccg ttgccataaa acataaagat ttatctggac agtacctcca
     1921 ggtaaagact ccactacgag attacgaatt caaattacca ttactcggaa ttcaccaaac
     1981 ggaaaacgcc cttactgcca tatgtgctat tgagacaata aaagaattgg gttatctaat
     2041 tgatggagat tcggttgaaa ctggactgtc ggaggtctgt tggcctggaa gattcgaagt
     2101 gttaaaaaca tcaggtccaa ttttagttgt tgatggcgct cacaacccgt attcaatgga
     2161 acgattggta gagactgtta acgaccatat gagtaactcc aaagtgatcg tagtttttgg
     2221 ggcgactggg ggccatgatc tcgccaatat gctggaacct ttgaagttac tggagccggt
     2281 tcttattcca gtatcttcaa ggcaccccaa atctatccag tcggaaattg tcagtaaagc
     2341 ttccttgaat tgcgatatcg ttactagcaa ggcatttggg agtgtttcca aaggtttaga
     2401 ccacgccttt agtttggcag ggcggaatga tttaatatta ggtacgggct ctctgtccgt
     2461 tgctgcagag gtttctgaag ttattaagga aatttcgcct gagatttatc caaatttgcc
     2521 ttaaaataac agatggaaat taaaatgagg acattagaag gtttataaat ataatggcaa
     2581 aagacaatac aagggtagta ataaccggac taggggtgat gtccccttta ggtcaaacag
     2641 tcgaggaatt ttgggatggc ttgtctaatg gacgctctgg cataggtaga atcacgttat
     2701 gtgatacaga agggtttccg agtgacatag caggggaagt tacaggattt gatgcagggc
     2761 aatatgcaga ccccaaagag gttcggagaa tggccagatt ttctcaattt gcagtggcag
     2821 ctgctgctac cgcaattgaa gattccgctt tagatttgtc atcaaataac gaccgagttg
     2881 gagttgtgat gggcaacggt aatggcggct ttcctactac agaagacaac gcaaagattc
     2941 tgtttgaaaa gggagggatg aaagtaagtc ccttttttat tccaatgata cttcctaata
     3001 tggctgcagc ccaggttagt cgagtattca atttaaaggg gtattcctcc acccttataa
     3061 ccgcatgtgc tgcgggtaat caggcgattg gagaggcata tgaggctatc aggagaggag
     3121 cagcggatgt catagtggct ggtggttgtg aggcaggtat aagcagaata ggtcttggtg
     3181 gatttcacgt tataagggcc ctaagtaggt ttgatggaga cccacaaggt gcgagtaagc
     3241 cgtttgattc aaaccgggat ggtttcgttc cggccgaagg agctggaata ctcataatgg
     3301 aaaccttaga ccacgctcaa aagagaggtg cggatataaa aggggaaatt gttggatatg
     3361 ctgtgtcctc tgatgcatat catgctgtgc agcctgataa aaatggtgaa ggcgctgctc
     3421 gagctatcac atgggctatt agtaatgccg acaaacgact agaagatatt gactatatca
     3481 acgctcacgg aacctcaaca ccactgaatg atgcttcaga gaccaaagcg atcaagcttg
     3541 cttttggtga tctagct
//
LOCUS       DASCNS010000263         2040 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001968215, whole genome shotgun sequence.
ACCESSION   DASCNS010000263 DASCNS010000000
VERSION     DASCNS010000263.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001968215"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="QF838_09430"
     CDS             <1..211
                     /locus_tag="QF838_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HJO60938.1"
                     /translation="DTRNYEGCEYISLSFDQDDENTFVMTEQWVSREHHRIYSEFREN
                     DGTAEIIAPLLIAEPTFRYLNLTDA"
     gene            complement(359..889)
                     /locus_tag="QF838_09435"
     CDS             complement(359..889)
                     /locus_tag="QF838_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60939.1"
                     /translation="MLVSSSNEITKPYSLKWFAGTLVGSGVAGTLVGSGVAGTLVGSG
                     VAGTLVGSGITVTVVGTRVTTVLAGLSLAVGTGFGPEGTGGWAAGVEGGTKTAGEGAE
                     ANSLVGASGESTSTARPTAGCPHPVITKSKVSTVISTADWPSFIKRLREDDILKYPHI
                     KLIILAESNVTFILIS"
     gene            888..1301
                     /locus_tag="QF838_09440"
     CDS             888..1301
                     /locus_tag="QF838_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60940.1"
                     /translation="MLDSNLSVTGLLSEDAEKDQGLMTFEYNGADIALSWLPQDDLTA
                     ADLVDLGYSLLVDSQPDKEFIPFASGDIEIDGQLGSFGGLVVSDSGGTEAGGGLIGGW
                     TCETQNTSFILMVTSPDSTVLQIRFDRMISGFECP"
     gene            1335..>2040
                     /locus_tag="QF838_09445"
     CDS             1335..>2040
                     /locus_tag="QF838_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60941.1"
                     /translation="MHRLLIVTAVLLFSISNFGSINAADPPPPAPAQAPGGGAGNQQG
                     QQGPAQQGKPADNLGQPGQQGKPAGQQGKPAGQQGKPAGQQGQQSGPAPSLFSAKGDF
                     KNSTKDDMKKMDKEAMAGLDKQHFKGMNREAMSGFDKDHFQKMDKEAMAGLDKDHIKN
                     IGKEAMGGFDKGHFQKMDKEAMAGLDKDHIKNIGKEAMGGFSKDHFQKMDKEAMAGLD
                     KDHIKNIGKEAMGGFDK"
BASE COUNT          606 a          429 c          493 g          512 t
ORIGIN      
        1 cgatactcgt aattatgaag gatgtgaata catttccttg tcgtttgacc aagatgatga
       61 gaacactttt gttatgacag agcagtgggt ttcaagggag caccacagga tatattctga
      121 atttcgtgaa aatgatggaa cggcagaaat aatagcccct ctgcttatag cggaaccaac
      181 tttcagatac ctaaatctca cagatgcata gctgatggag tatggataaa tccacataag
      241 tgtagccacc cacatgtaaa taccgttgta attgggtact aacagcttat agtattttat
      301 taatgctcaa atagtaatta ttgaatttta tgtgttttag caaaatgatc agtgtgaatc
      361 agctaataag aatgaaagtc acgttgcttt cggcaaggat aattaacttt atatgaggat
      421 attttagtat gtcgtcttct ctcagccttt ttataaagct aggccaatcg gctgtactaa
      481 tgactgtgct caccttactt tttgtgatta cgggatgtgg gcaacctgca gttggacggg
      541 ctgttgaggt cgattcacct gaggccccca ccaaagaatt agcctcggct ccctcaccag
      601 cagttttggt tcctccttcc acgccagcag cccaacctcc ggtaccttcg gggccgaaac
      661 cagttccaac tgccaaactt aatccagcca agactgttgt gactcttgtg ccgaccacag
      721 tgactgttat accagaaccc accaaggtgc ctgctacgcc agaacccacc aaggtgcctg
      781 ctacgccaga acccaccaag gtgcctgcta cgccagaacc caccaaggtg cctgcgaacc
      841 attttagtga atatggtttt gttatctcat tagatgaaga taccaacatg ttagactcca
      901 atctctctgt gacgggtctc ctttcagaag atgctgaaaa ggaccagggt ttaatgacct
      961 ttgaatataa tggggctgat attgccctgt catggctacc tcaagacgat ctaactgctg
     1021 cagatttggt tgatcttgga tactctttgt tggttgacag ccaacctgac aaagaattta
     1081 ttcccttcgc tagtggtgat atagaaattg atggccaatt aggaagtttt ggaggactag
     1141 ttgtctccga ttctggtggt acagaagccg gaggtggtct tataggtggt tggacatgtg
     1201 aaactcaaaa tacttctttt atattaatgg taacgagccc tgactccact gtattgcaga
     1261 taaggtttga ccggatgata tccggctttg aatgcccata aaaaagacat aaaaaagaat
     1321 tgagggatag aaagatgcat agactgttga ttgttactgc ggtacttctg tttagtatct
     1381 ccaacttcgg ttcaataaat gccgccgatc caccgcctcc agctccagct caggctccag
     1441 ggggtggagc tggaaatcag cagggccaac aggggccagc ccagcaaggt aagccagcag
     1501 acaacctagg tcagccaggc cagcaaggta agccagcagg ccagcaaggt aagccagcag
     1561 gccagcaagg taagccagca ggccagcaag gtcagcaatc aggacccgcc ccgtcacttt
     1621 ttagtgcgaa aggagatttc aaaaattcaa ccaaagatga tatgaagaag atggacaagg
     1681 aagccatggc tggtcttgat aaacagcatt ttaagggtat gaatcgggag gcgatgtcag
     1741 gttttgataa agatcatttc cagaagatgg acaaggaagc gatggctggg ttagataaag
     1801 atcacatcaa gaacatagga aaagaagcca tgggtggttt tgataagggt catttccaga
     1861 agatggacaa ggaagcgatg gctggccttg ataaagatca catcaagaac ataggaaaag
     1921 aagccatggg tggtttcagc aaagaccatt tccagaagat ggacaaggaa gcgatggctg
     1981 gccttgataa agatcacatc aagaacatag gaaaagaagc catgggtggt tttgataagg
//
LOCUS       DASCNS010000264        15274 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001968560, whole genome shotgun sequence.
ACCESSION   DASCNS010000264 DASCNS010000000
VERSION     DASCNS010000264.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 15274)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15274)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15274
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001968560"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(100..894)
                     /gene="surE"
                     /locus_tag="QF838_09450"
     CDS             complement(100..894)
                     /gene="surE"
                     /locus_tag="QF838_09450"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HJO60942.1"
                     /translation="MRALLTNDDGIHSPGLWAIAKSLEEIADLTVVVPDRDQSGKGAS
                     MTLLRPLTVEKVDTPENMKCPTYTVDGTPGDCVIMACESLCMEPIDLIVSGINQGANM
                     GLDVFNSGTFGAALHGYFRGINSIALSVFYRQDVIYSPAAIVGAEIAKIVHSQNFDST
                     VLLNVNLPACDLKDIKGVDLTKLGPKAYLENVETVESGRRTFHWLRHNKPMEVTYQEG
                     TDVWSINHDRASITIIDPYLTTNNNTELGNLVLMGSINSLGVRPSD"
     gene            complement(1064..2035)
                     /locus_tag="QF838_09455"
     CDS             complement(1064..2035)
                     /locus_tag="QF838_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HJO60943.1"
                     /translation="MNNSITDIPGIAVGHWTNLESITGCTAILCPTGSLGGVSVRGAS
                     PGTRETDVLHPSNRVDEIHAIMLSGGSAFGLAAADGAMRYLEGKGIGVKVGGSIVPIV
                     PSAILFDLGIGEGGVRPDSESGYYACEFASSDPVEQGCVGAGTGATVGKTQGPQRSVK
                     GGLGSASIDLGEGLLVGALVVVNAIGSVHDPENGSLIAGPRDNKSMSSSINDLISGNS
                     FKLPDINGNTTIGVVVTNAKLTKAQAARMAASSHDGLALAIRPSHLIGDGDTMFSIST
                     GEQGGIDGYGDMNRIIAGTIKTVSLAILNAINFAETMGGVPSVKDVS"
     gene            2456..4402
                     /gene="gyrB"
                     /locus_tag="QF838_09460"
     CDS             2456..4402
                     /gene="gyrB"
                     /locus_tag="QF838_09460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HJO60944.1"
                     /translation="MATLKPQNDDQYSASDIQVLEGMEAVRKRPGMYIGSTDQRGLHH
                     LIYEIVDNSVDEFMGGYCSSVNVTIQEDGSVLVTDDGRGIPVDKHPATGMTALETVMT
                     TLHAGGKFGGKAYAVSGGLHGVGASVVNALSKWVKVAVYREEKIFRQEYSRGITQGEM
                     TSENDPDGNNSATGTTIEFMPDIDIFGELVFDFAVLSNRFKEMAYLNKGLEISFKSDW
                     HASRWPNNEVTYYFDGGISSFVRGLNQKRGVVHEEPIYVEKDFEGTKVEVALQYNDSY
                     SEFVYAFANCINTEDGGTHVTGFRSALTRVLNDFGRKSKLIKDNEPNLAGEDTREGIT
                     AVISVKLKDPQFEGQTKNKLGNPEARTQVETVLGEALQIFLEDNPKEAKAIIDKCMTS
                     QRAREAARKARELIIRKNAMDGGGLPGKLADCSEKNPELCEIYLVEGDSAGGTAKMGR
                     NRRFQAILPLWGKILNVEKARADKIVEHDAIRSMITAIGAGLDEDFDVEKLRYHRVII
                     MCDADVDGSHIRTLLLTFFFRHMPELIHHGHLYIAQPPLYKISVGRKDQYVYTDAERE
                     EVLEQLDGKRNVHIQRYKGLGEMNSDQLWDTTMNPESRTLLQVGVADAVIADDTFTIL
                     MGDEVAPRRNFIQTHALEVTNLDI"
     gene            4500..5402
                     /locus_tag="QF838_09465"
     CDS             4500..5402
                     /locus_tag="QF838_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HJO60945.1"
                     /translation="MESERVAYVNGKIIQESLASISIHDRGFLQGYSVFDTTRTFNHR
                     IFKLEEHLNRFYNSLKYARLDPGMPRTKMAQITMEVLETNLPLIDNDDDYWVTQRVSR
                     GVSGPDGETPTVIVETVPLPFKARAKYYRDGMPVVVPSVRRTPPEVLSPRAKIQNYAN
                     LVQAEFEVKDKNPDGWPILLDMNGNLCEGPGSNFFIVKDGVVVTPREQFVLAGISRST
                     TIELAQELGIETREADIDLYDAYTADEAFVTSTSFCICPVSSINGSSIGDSGIVPGPL
                     TSRLSDAYSGLVGIDIAGQYLSRL"
     gene            complement(5466..6386)
                     /locus_tag="QF838_09470"
     CDS             complement(5466..6386)
                     /locus_tag="QF838_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HJO60946.1"
                     /translation="MPYAQSIQDFVNQYSALKAIGNTPLVKLDLPWDIGNSCIHAKFE
                     QLNPGGSIKDRPVLRMLAEAVISGKLKQGKTILDATSGNAGIAYAMVGAILGFSVELV
                     MPENASEERKKRLKAHGANLIFTDAQLGYDEALYEVKRRYAQNPDRYFYCDQYSNNNN
                     PLAHYETTAVELLEQAPFVTHFVGGVGTGGTISGIGRRLKETNPSIQVICIDFEEWPG
                     VEGLKPLSEGHIIPETFDSSVVDRMITVDVVEGYEISNELAKKGIFVGQSSGAYLLGA
                     KQVAEEVRSGHVVTIFNDIGERYFSTSMWG"
     gene            6572..8584
                     /gene="ligA"
                     /locus_tag="QF838_09475"
     CDS             6572..8584
                     /gene="ligA"
                     /locus_tag="QF838_09475"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HJO60947.1"
                     /translation="MDRKEAANRITHLRTVIDRHNYLYFVENVPEIGDGQYDKLMSEL
                     RDIENTFPDLITTQSPTQRVGAAPLIGFSEISHEIPMLSLSNAFSDDDLTAWYHRLYS
                     MLDDTNFEMACELKYDGLAVSLRYENGLFIQGATRGNGMTGEDITLNLKTINSIPLQL
                     QGEFPQTLEVRGEVYFPKSEFDKFNARRDQEGLHTYANPRNTAAGSLRQLDSNVTAGR
                     PLDVFIYSLAQMDSDLSLGTQLKTLNYLRSLGFKINPHNILAKDTQEVIKYHTSWIQN
                     LASLDYDCDGIVVKVNRLDYQQHLGTIGREPRWATAYKFPAVQAQTRLLDIRLNVGRT
                     GSINPYAVLDPVEVGGVIIKQATLHNADYIESKDLKIGDWVVVERAGEVIPQIVRSDK
                     DKRTGAETYFAMPAICPSCGTILVNRKDEAATYCTNSTCPAQLERLVEHFAGKGAMDI
                     EGMGGKVGISLIQNGLIKDVADIYFLQRERLEQLDRMGKKSVDNLLIAIDESKKQPPE
                     RLLVSLGIPHIGSEISSLLLNQFASIDNLVACNQETLLNTPSVGPKISESITEYFGNP
                     DNLKILDKLRSAGLNMVSETVIPSEGIPLLSDLRFVVTGRLENYSRTEIQDLIKKYGG
                     KVSSSVSKSTDYLLAGQDGGSKLAEAAKLEVQIISEHDFETMVAGN"
     gene            complement(8606..9553)
                     /locus_tag="QF838_09480"
     CDS             complement(8606..9553)
                     /locus_tag="QF838_09480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJO60948.1"
                     /translation="MTKLGVLLPTRGLLMTDDLPTNIDEIVDMAESVEASGIDSIWVG
                     DSLTAKPRLEPFTALSAIATRTKNIRLGTAVLLSSLRHPVQLAHVATTLDLISEGRLV
                     LGMGVGGAFNDAQRKEWQNVGVDPRKRAGRFEESLKIFKPLTRGETITFSGKHFDVKD
                     ISIKPVSPQTNGIPVLVAAHGRSKLERQYERVLLGDGIISISDFPDEYEMALKKVSQY
                     CEQKGSPFKNLEKSFYMTVNIGENKEKLTEEADRFLKLYYGVNIWQERWGPWGAPEEV
                     LTRIKSYENVGAETIIVRFASFDQAGQLDLFLNKVATYL"
     gene            complement(9581..10201)
                     /gene="cofC"
                     /locus_tag="QF838_09485"
     CDS             complement(9581..10201)
                     /gene="cofC"
                     /locus_tag="QF838_09485"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HJO60949.1"
                     /translation="MDQFSVLIPMKIPSEGKSRLAGVLDEPSRKLLIISLLERVLEAS
                     MNSSSCDVYVIGGGFEVADICRRIGANWLNSDSGDLNCDVSRGIAEIAQAGNGTIYLP
                     GDLPFVTAEDIDSVIFRSENGDLAVIVESESDGGTNCVLFPYQTKLIPLLGSDSYRKH
                     CEYAVNHGILFESIRPAGLLIDLDTTNDLIKCQRKEKGFLRNVGII"
     gene            complement(10206..11135)
                     /gene="cofD"
                     /locus_tag="QF838_09490"
     CDS             complement(10206..11135)
                     /gene="cofD"
                     /locus_tag="QF838_09490"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HJO60950.1"
                     /translation="MKSVVALAGGVGGAKLALGFSYILGSEELTVVVNTADDDCFYGL
                     HISPDVDTVLYTLAGVSNAEMGWGLAGESFRTLERLKEYGVDAWFNLGDLDLATHLYR
                     TKMLDEGKTLSEVCQRLAKSLGVEHKILPVTDSSIKTILETQDGIMSFQEYFVKNRCE
                     PIVKSIDFDGSSNCTVTPQVREAVLDMDLLVFCPSNPFVSLAPILSVPGFKEMIETFS
                     GKSIGVSPIVGGAAIKGPAAKMLKELGHDVSSVGVARQYVGLCNTFIIDEKDVALKEE
                     IESLGMDVFVTQTIMETDQDKKQLAEYILEISA"
     gene            11248..12420
                     /gene="recF"
                     /locus_tag="QF838_09495"
     CDS             11248..12420
                     /gene="recF"
                     /locus_tag="QF838_09495"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HJO60951.1"
                     /translation="MLWRILIRISHLSLLNFRNYDSLELALPPGTTVIQGANGHGKSN
                     LLEAIYMMAIAKSPKVTTDRELINKSSVLQLNQQELSYSKIGCVTEHTQGSENLEIHY
                     RCQLSQNIHGFVAQKFIRINGSPKRSSELVGIVNAVLFSVGDLDLIYGSPSVRRRYLD
                     ILLSQVDKEYLSSIQQYQKANTQRNHLLRLIRDGSSSANELTFWDQQLAKNAVLLTRK
                     RQEAVKILEGASLPFHRLMGGRNEKMSIRYARTDHLSEYIDLENFLKDLEESRRKDIA
                     QGSTSIGPHRDDLIFAIDNMEASKYASRGQSRTAILALKMAESEFLYARRTTKPVLLL
                     DDVMSELDQTRREKLVEHLEKHDQVILTTAEPSNIPESFVNSSNRLEVLNGSVKTI"
     gene            12433..13251
                     /locus_tag="QF838_09500"
     CDS             12433..13251
                     /locus_tag="QF838_09500"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HJO60952.1"
                     /translation="MEIKKQKSISAAAKIIVQSRYTIALIGAGLSVESGIPTFRGAGG
                     LWTKLGEPPNNGYENFLADPKAWWEQNLNSEVDPERTQFREAIEKAKPNPGHLALVNL
                     EKLNILKHQITQNIDNLHHIAGSKNVTEIHGNRIKLRCVNCDMRWHRDEFDQIRLDWK
                     QNLPPKCNTCLGIVKPDTVMFGEPIPFSTLNTCVNETRLADCILVIGTSATVYPAAGF
                     PREVSSSGGKIIEINPEETPISEAATESIKGPTEDSLPKLVAEIKGIIGDDPAS"
     gene            13659..15188
                     /locus_tag="QF838_09505"
     CDS             13659..15188
                     /locus_tag="QF838_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HJO60953.1"
                     /translation="MKYDKIVIGAGSAGAVLASRLSEDPDCSVLLLEAGPDYPDFGTL
                     PFDLKHGWGTGPDIVVGGIHDWQFTGTASTLSKTMPIPRGKVTGGTSAINGQVFLRPL
                     PEDFERWVEWGNDRWSFKECLPYLLKIETDLDYDGDFHGQDGPILVKRHPLNSLTDDQ
                     LAFYKACKYLGYEENIDHNLPDATGVGPYPLNNPDGIRFSTALGYLSQSRHRLNLTIK
                     PNCVAQKIVFDRNVATGVEVISGGEIFKVEGTEVILSAGSIATPQILMLSGIGSEKLL
                     SPFHISVVSDLPGVGKNLRDHPTVHTTWTPHSSFNKPKQKIGPQKVALRYTSQDSKYQ
                     NDMIMIMRFSHEIGDVGALMISVGIYLAESVGRISLQSTDIHTQPILDYNLLSHKYDL
                     KRLREGVRLADKIAKAPSFTDTIVARLSPDEITINNDDLLDRWMLENVLTMHHISGTC
                     KMGPKSDAEAVVDQYGVVYGTEQLRIADASILPDCPRANTNVAAMLIGERIADFIKTG
                     D"
BASE COUNT         4784 a         3372 c         2963 g         4155 t
ORIGIN      
        1 aaatcaaaag gcattcattg ctactcccag aaaatactta actcaaaagc cactaatgcc
       61 aaaaacttat aacattgctt tacttggtcc tcaaaatacc tagtctgatg gtctcactcc
      121 cagactatta atagagccca tcaacaccaa attacctaat tcagtattat tattggttgt
      181 gagatagggg tcgatgattg taattgacgc cctgtcatgg ttaatggacc aaacgtcagt
      241 tccttcctgg tatgtgactt ccattggttt attgtgacgt aaccaatgaa atgttcgtct
      301 accactttca accgtttcca cattttctaa atatgccttt ggtcccagct ttgtcagatc
      361 taccccttta atatctttca aatcacaggc agggaggtta acgtttaaca aaactgtcga
      421 atcgaagttt tgactgtgca ctatcttggc gatctcagct cccactatgg cagcagggga
      481 atatataaca tcctgcctat agaaaacaga taaggcgata gaattgattc ccctaaagta
      541 tccgtgtagt gccgcaccga aagtgccaga attaaaaaca tctaacccca tatttgcacc
      601 ttggttaata ccggaaacta tcaaatctat gggttccata cataaagatt cacaggccat
      661 gatgacacag tcccctggag taccatctac tgtgtatgta gggcatttca tgttttcagg
      721 ggtatcgact ttctcaaccg ttaaaggacg taacaaagtc atggaagccc ctttaccact
      781 ctggtctcta tctggaacaa ccacagtaag atctgctatt tcctccaaag acttggctat
      841 ggcccagagt ccaggagaat gtatcccatc atcatttgtt aacagcgcgc gcatagagac
      901 ccaacctttt taaattaccc ttttatcaaa cagtaacaga cattataaag ccagagtctg
      961 acaaaaaatt acttcttttt tgaaattact ttatcgatta gtcaggcggt tccggcattc
     1021 acagacacac aaattgttga tcctgatgtt acagaatttt ctgctatgaa acatccttga
     1081 ccgagggaac tccacccata gtttcagcaa aattgatagc attcagtata gctaatgaca
     1141 ccgtcttaat agttcccgcg ataatgcgat tcatatcacc atatccgtca atgccacctt
     1201 gctctccagt ggatatagag aacatagtgt caccatcacc aattagatga gaaggtctga
     1261 tagctaaggc caaaccatca tgcgaactgg ctgccatccg tgcagcctga gctttggtca
     1321 gctttgcgtt agttactaca actccgatgg tggtattacc atttatatct ggcaacttga
     1381 aagaatttcc agatatcaag tcattaattg agctagacat ggatttgtta tctctagggc
     1441 ctgctatcaa tgatccattt tctggatcat gtacactccc tatagcattt acaacaacca
     1501 acgcccctac caataaacct tcacccaaat caatgctggc cgatcctaat ccacctttca
     1561 cggatctttg tggtccctgg gttttcccca cagtcgcacc tgtcccagct ccaacacacc
     1621 cttgttctac aggatctgag gaagcaaatt cacaggcata atatccgctt tccgaatccg
     1681 ggcgcacacc cccttcccca attcccaaat cgaataaaat tgccgacgga actataggaa
     1741 ctatgctacc accaactttt actccaattc cctttccctc caagtatcgc atcgcaccat
     1801 ctgctgcagc taaaccgaaa gcactgcctc ctgaaagcat aattgcatga atttcatcta
     1861 cccggttaga ggggtgtaga acatcagtct ctctagtccc tggggaagcc cctcgaactg
     1921 atactccacc caaagaacct gttggacaca atatagctgt acaccccgtt atggactcta
     1981 gattagtcca atgccccacc gcgatacctg gaatatctgt aatactattg ttcatgtttt
     2041 ggtaccaaaa aataaggtcc gcttttttga aaatagcaga cccttaaaaa atcatctttc
     2101 atcgacataa taatacattt ttccaaggct caccatctct ttaaaggttg gctgcgcaga
     2161 ccaattttat caattaagtc aataacaata cagatactcc aaatacgggc aaatatttcg
     2221 gctaatatta ggaacaaatt acgcttgagt aaaccatcat gattctgcta gaattgaaat
     2281 cgtgaaatta gagactttct catggttact acatcaacca cttagacagg gcatacgtgc
     2341 ccgtattaac gtctcagaga cggcaattcc tcaccctatt tttacaaagg acgcatcctt
     2401 aaatgcaagc agatctataa ctcgtccaat aggctctttt tagggagatt cgcaaatggc
     2461 aactttgaag ccccaaaacg acgatcagta ttcagcaagt gatatccaag tccttgaagg
     2521 tatggaagcg gtccgtaaaa gacccggtat gtacattggg agcacggatc aaagaggcct
     2581 ccatcacctg atatacgaga ttgtcgataa cagcgttgat gaattcatgg gtggatactg
     2641 cagttcagta aatgtaacta tccaagaaga tggctctgtt ttagtaactg atgacggccg
     2701 gggtatccct gtcgataaac atccagctac tgggatgaca gccctagaaa ctgttatgac
     2761 cacattgcat gccggtggaa aatttggagg caaggcatat gcagtctcag gtggactaca
     2821 tggcgttggc gcctcggttg taaatgccct gtccaaatgg gtgaaagttg ccgtatatag
     2881 agaagaaaag atattccggc aagaatattc tagaggtatc actcagggtg aaatgacctc
     2941 agaaaacgat ccagatggta acaacagtgc tactggcaca accatagagt ttatgccaga
     3001 catcgatata tttggtgaac tagtgtttga ttttgccgtt ctttccaaca ggttcaagga
     3061 aatggcatat cttaacaagg gtttggaaat ttctttcaaa agtgactggc acgccagccg
     3121 ttggccgaat aacgaagtaa cctactattt tgacggaggt atatctagtt ttgtcagagg
     3181 cctgaatcaa aaaagaggtg tggttcacga ggaacctatt tatgtggaaa aggattttga
     3241 gggtaccaaa gttgaggttg cccttcaata caatgattcc tatagcgaat tcgtatacgc
     3301 attcgcaaat tgtataaaca ctgaagatgg agggacccac gttaccggtt tcagatcagc
     3361 tctaactcgg gtcttgaatg actttggaag aaaatcaaaa cttattaaag ataatgaacc
     3421 taatcttgca ggagaagata caagagaagg tattacagca gtaataagtg tcaaattgaa
     3481 agatcctcaa tttgagggac agacaaaaaa caaacttgga aatccagaag ccagaactca
     3541 ggtagagacc gtattaggag aggccttaca aatattccta gaagacaatc ctaaagaggc
     3601 gaaggcaatc atcgataaat gtatgacctc acaacgggcg agagaggctg ctaggaaagc
     3661 cagagagtta atcatccgta aaaatgccat ggatggcggt ggccttccag ggaaactcgc
     3721 tgattgctca gaaaaaaacc cggaactttg tgaaatttac ttggtggaag gagattcagc
     3781 tggcggtaca gcaaagatgg gcagaaaccg aagattccaa gcaattctac cgctgtgggg
     3841 aaaaatccta aatgtagaga aagctagagc agataaaata gtagaacacg atgccatccg
     3901 atcaatgatt acagcaatag gggctggtct tgacgaagat ttcgacgttg agaaacttag
     3961 ataccaccga gtaattatca tgtgtgatgc ggatgttgat ggctcccaca tacgtaccct
     4021 actcctgact tttttcttcc gacatatgcc ggaactgatt catcatggtc acctttacat
     4081 cgcccaaccc cctctatata aaatatccgt tggaagaaaa gaccaatatg tttataccga
     4141 tgccgaaaga gaagaagttc ttgagcaact ggatgggaaa agaaatgtcc atatccaacg
     4201 ttacaaaggc ttgggtgaaa tgaactccga tcagctgtgg gacactacta tgaatcctga
     4261 gagtcgtaca ttgttgcagg tcggtgtggc tgacgcagtg atagcagatg atacctttac
     4321 tatactgatg ggagatgaag tggcaccgag aagaaacttt atccaaactc acgctctgga
     4381 agtgaccaac ttagacattt aaaccggtga tgtttggata aaactcgcgc acccggtatg
     4441 atctatgact ttacgacagc atccagatag aagagcgtat tcacgactgg agattgatga
     4501 tggaatcgga aagagtagct tatgtaaacg gtaaaataat acaagagagc ctcgcctcca
     4561 tctcgattca tgatcgaggt tttttacaag gctattctgt ttttgatacc acccgtacat
     4621 ttaaccatcg gattttcaaa ctagaagaac acctcaatcg tttttataac tcccttaaat
     4681 atgcccgatt ggatcccgga atgcctcgca ctaaaatggc tcaaataact atggaagttc
     4741 ttgagaccaa tcttcccctt attgacaacg acgacgacta ttgggttaca caaagagttt
     4801 ccaggggagt atctggcccg gacggagaaa ccccaacagt gatagtggaa actgttccac
     4861 ttccttttaa agcacgcgca aaatactaca gggacggaat gcccgtagtt gtcccatccg
     4921 tccgaagaac accgccggaa gtactaagcc caagggccaa aatacaaaat tacgctaatc
     4981 tcgttcaagc agaatttgaa gtgaaagaca aaaacccaga tgggtggcca attttgttag
     5041 acatgaatgg aaatctttgt gaaggaccag gaagtaattt ttttatagtg aaagatggtg
     5101 tggtggtgac cccaagggaa cagtttgttt tagcaggtat cagtagatct actactattg
     5161 aactggcaca agaactcggc attgaaacca gagaagcaga tatcgatttg tatgatgcct
     5221 acactgcgga tgaagctttc gtcacttcaa ctagcttttg catatgccct gtatcatcta
     5281 taaacggatc tagcatcggt gattcaggaa ttgttccagg tcctttaact tctcgactgt
     5341 cagacgctta cagtggatta gtgggaatcg acatcgctgg acagtatctt tctagattgt
     5401 aactggtata caaagcagag aaaaacatcc taacaagata cttgaactcg tcgaagatgt
     5461 ttaagtcatc cccacatgga ggttgagaaa tatcgttcac cgatatcatt aaaaatagtc
     5521 accacatggc cagatctgac ttcctccgcc acctgcttgg caccaagcag ataagcccct
     5581 gatgactggc cgacgaaaat tcccttcttt gccaattcat tagatatttc atatccctcc
     5641 acaacatcca cagtgatcat gcgatccact actgaactat caaaggtctc aggaataata
     5701 tgcccctcgc ttagcggttt tagtccctct actccaggcc attcttcaaa gtctatacaa
     5761 atcacctgta ttgacgggtt cgtttctttc aaccttcggc caatcccact aatggtacct
     5821 ccagtaccga ccccaccaac aaaatgagta acgaaaggtg cttgttccaa aagttctaca
     5881 gccgtagttt cataatgagc caacggattg ttattattac tatattggtc acagtagaaa
     5941 tatctatcgg ggttttgagc gtaccttctt ttaacctcat ataaggcttc atcgtatcct
     6001 agttgtgcat cagtaaatat gagattcgcc ccatgggcct ttaacctttt tttcctttct
     6061 tcactggcat tttctggcat caccaactca accgaaaatc ccaaaatcgc tcccaccatg
     6121 gcgtatgcta tcccagcatt acccgacgta gcatcaagga tagttttacc ctgtttcaat
     6181 tttccagaaa tgacggcttc tgccagcatc cgcaacactg gcctatcttt tattgagccg
     6241 cccggattta attgttcaaa cttagcgtga atacatgaat tacctatatc ccacggaaga
     6301 tccagtttaa ccaatggggt attcccgata gctttcagag cggaatattg attcacaaaa
     6361 tcttggatac tttgagcgta aggcaagggt ttaatcggcc tctagttttg acaaaaatta
     6421 aagaagaagt aattctatct cctcaatgag tatatcttct gataattttg cagtctagca
     6481 gagaaaaagg aacggatgta gccgtatgta cccttgagca cttttttcca tcaagttagt
     6541 atgaagtaac caaggctctg atcggggatt tatggataga aaagaagccg caaatagaat
     6601 aacccactta cggacggtaa tagaccgtca taattaccta tatttcgtgg aaaatgttcc
     6661 tgaaattgga gatggtcaat atgacaaatt gatgtcagaa ctacgggata tagaaaatac
     6721 cttcccagac ctaattacca cccaatctcc cacacaaaga gttggtgccg caccacttat
     6781 tggattttca gaaatatcac atgaaatacc gatgcttagc ctttcaaacg ccttctcaga
     6841 tgatgattta acagcatggt accaccggct ctactcgatg ttagatgata caaatttcga
     6901 aatggcctgt gaacttaaat atgatggact agcagtctca ttacgatacg agaatggatt
     6961 atttatccaa ggtgcaacgc gaggaaatgg aatgacagga gaggacataa ctttaaacct
     7021 caaaaccatt aatagcatcc cactacaatt acaaggggaa tttccacaaa cccttgaagt
     7081 tagaggtgag gtttatttcc cgaaatctga atttgataaa ttcaatgccc gcagagatca
     7141 ggaaggactt cacacctatg ccaacccccg caacaccgct gcaggatcct tgcggcaatt
     7201 agattccaat gtgactgctg gcagacctct cgatgttttc atatattcgt tagcacaaat
     7261 ggatagcgac ctcagtcttg gcacacaact taagaccttg aactatctga gaagcctagg
     7321 gttcaaaata aatcctcata atattttagc aaaagataca caagaggtca tcaaatacca
     7381 caccagttgg atccaaaatc ttgcctcgtt agattatgac tgtgacggaa tagttgtaaa
     7441 agtgaaccgg ttggattacc aacagcattt aggaactata gggagggagc cgagatgggc
     7501 gacagcttat aaatttccag ctgtccaggc ccaaacaaga ctcctagaca taaggttaaa
     7561 tgtgggaaga actgggagca taaaccctta tgctgtactt gatcccgtag aggttggagg
     7621 tgtcattata aaacaggcaa ctttgcacaa tgcggactat atagaatcca aagatctaaa
     7681 aattggagac tgggttgtag tggaaagagc cggagaagtc atcccgcaga tagtcagaag
     7741 tgataaagat aaaagaaccg gtgccgaaac atactttgca atgccagcca tatgcccaag
     7801 ttgtggcact atattggtaa acagaaaaga tgaagcagct acctactgca caaattctac
     7861 ttgccctgca cagttggaaa gactcgttga gcactttgca ggaaaaggtg ctatggatat
     7921 tgaaggaatg ggaggaaaag taggtatttc cctaatacaa aacggactca ttaaagacgt
     7981 agcagatata tatttcttgc aaagggaaag acttgaacaa ttggatcgga tgggtaaaaa
     8041 aagtgttgat aacctcctta tagctataga tgaaagtaaa aaacaacctc ctgaaagatt
     8101 attggtctct ttaggcatcc cacatatcgg gtcagagata tcatctttat tattgaatca
     8161 attcgcctcc atcgataatt tagttgcctg taatcaagaa actctcttga atactccttc
     8221 tgttggtcca aaaatatccg aaagtattac tgagtatttt ggtaatcctg acaatctaaa
     8281 gattcttgat aaattacgat ctgcgggatt aaatatggta tctgaaaccg tcatcccttc
     8341 tgaaggcatt cctttgctgt cagatctccg atttgtagtc acaggaagac tagagaatta
     8401 cagcaggaca gagatccaag acttaattaa aaaatacgga ggaaaggtta gtagttcagt
     8461 ttctaaaagc acagattatt tattagctgg acaagatgga ggttcaaaac tggctgaagc
     8521 tgctaaattg gaagtccaaa taatttcgga acacgacttt gaaacaatgg ttgcaggtaa
     8581 ttgaaatctt aatccatcat atcctttata agtaggtcgc gaccttattc agaaacaggt
     8641 caagctgccc agcttggtca aatgaggcaa acctaacaat tattgtttca gcaccgacat
     8701 tttcatagct ttttattctg gtcaaaacct cttcaggagc cccccaaggg ccccatctct
     8761 cctgccatat attcacccca taatataact tcagaaaccg atctgcctcc tcggtcaatt
     8821 tttctttatt ctctccgata ttcactgtca tataaaagct tttttctaaa tttttaaatg
     8881 gcgaaccttt ctgctcgcaa tattgggaca ctttctttag ggccatctca tattcatctg
     8941 gaaaatctga tatcgatatt atcccatcac caagaaggac ccgttcatat tgtctctcca
     9001 atttggaacg accatgggca gcgaccaaaa ctggtattcc attggtttga ggggaaaccg
     9061 gtttaatgga aatatctttc acgtcaaaat gcttcccaga aaacgtgatc gtctctcccc
     9121 tggtaagggg tttaaaaatt ttaagggact cttcaaacct accagccctc ttacgagggt
     9181 ccactcctac attctgccac tctttcctct gagcatcatt aaacgccccc ccaactccca
     9241 taccaagaac aagcctgccc tctgatatta aatccaacgt agtcgctaca tgagcaagct
     9301 gaacaggatg tcttaaagaa gaaagtaaca cagccgtacc cagtcgtata ttttttgtcc
     9361 tagtagctat agccgataaa gccgtgaaag gctccaatct aggttttgca gtcaaactat
     9421 cccctaccca aatagaatca atcccagaag cctctacgga ttccgccata tcaacgatct
     9481 catctatatt agtaggaaga tcatccgtca ttaacaatcc cctggtagga agtaacacac
     9541 ctaatttagt catatttaaa cccactttaa ccaagtattt tcaaataatt ccaacattcc
     9601 ttaagaatcc tttttctttg cgttggcatt ttattaaatc attcgtagta tcaagatcta
     9661 taagtaatcc cgctgggcga atcgattcaa aaagaatgcc atggtttaca gcgtactcgc
     9721 agtgcttcct ataactatct gaacccagca aaggaattag tttagtttga tatggaaaaa
     9781 gcacacagtt agtgccacca tcagactcag attcaactat aactgctaaa tcgccatttt
     9841 cagagcgaaa gatcaccgag tcaatatcct ctgcggtaac aaacggaagg tcccctggca
     9901 aatatatcgt tccattccct gcttgagcta tttcagcaat gcccctagat acatcacaat
     9961 ttaaatcccc cgaatctgaa ttcaaccaat tggcaccaat acgcctacag atatcggcta
    10021 cttcaaaacc tcctccaatt acataaacat cacagctaga tgaattcata gaggcttcaa
    10081 gaactctttc caacaaggat attattagta acttcctact tggttcatct aaaacaccag
    10141 ccagtctaga tttaccttca ctaggaattt tcatcggtat aagtacagaa aattgatcca
    10201 cttatttacg cgcttatttc tagtatgtat tcagctaatt gttttttgtc ttgatctgtt
    10261 tccataatag tctgggttac aaaaacatcc attcctagag attctatctc ttccttaaga
    10321 gctacatctt tttcatcgat aatgaatgtg ttgcagagcc caacgtattg gcgagcaact
    10381 cccacagagg atacgtcatg accaagttcc ttaagcatct ttgccgcagg acccttgatc
    10441 gctgccccgc caacaatggg actaacacca atagatttcc cagaaaacgt ctcgatcatt
    10501 tccttaaatc ctggcaccga taggattgga gcaagactaa caaaagggtt tgaggggcaa
    10561 aacaccagta aatccatatc tagaacagcc tctcttacct gaggggtaac tgtgcaattt
    10621 gaggatccat caaaatctat tgatttgaca ataggttcac accgattttt cacaaaatac
    10681 tcttgaaagc tcatgatccc gtcttgtgtt tccaatattg tctttatgga gctgtcagtc
    10741 accggcaata ttttatgctc aacacccaaa gattttgcta atcgctggca tacctccgac
    10801 aatgtttttc cttcatctaa catttttgtg cggtagaggt gggtagcaag atctagatcg
    10861 cccagattaa accaagcatc aacaccatat tccttcaatc tttctaaagt tcgaaaagat
    10921 tcccctgcaa gcccccaacc catttccgca ttggatacgc ctgctaaggt atatagaaca
    10981 gtatctacgt caggggaaat atgtaatcca tagaaacagt catcatcagc agtattgacc
    11041 acaactgtca actcctcaga acctaaaata taagaaaacc caagggccaa tttagctcct
    11101 ccaactccac ctgccaacgc aactaccgat ttcatcaata ctaatctcct aaaccttcaa
    11161 aaacttttca gaataactat ataactcaca cgatataact aacatgctaa aatccttcca
    11221 ataacagcgg attacgacac actgcacttg ctttggagaa ttctcattcg catctcccat
    11281 ttaagccttc ttaattttcg aaattatgac agtttggaac tcgcgttgcc tcctgggacc
    11341 acagttattc aaggagcaaa tggccatggc aaaagtaatc ttctagaggc aatctatatg
    11401 atggccattg ccaagtcacc aaaagtcacc actgacagag aattaatcaa caaatcctct
    11461 gtactacaac tcaaccagca ggaattgagt tactccaaaa tcggatgtgt tactgaacac
    11521 acccagggat cagaaaatct cgagatccat tataggtgcc agctatcaca gaatattcac
    11581 gggttcgttg cacaaaaatt tatacggatt aacggttctc caaaaagatc ttccgagttg
    11641 gtaggcattg ttaacgccgt tttatttagc gttggtgacc tagacctaat atatggtagc
    11701 ccgtctgtga ggcgtagata tcttgacata ctactttctc aagtcgacaa ggaatatctt
    11761 tcatccatac agcagtatca aaaagcaaat actcaaagaa accatctttt gagattgata
    11821 cgggacggct cctcttcggc aaacgaacta acattttggg accaacaatt agcaaaaaat
    11881 gctgttcttc tcacaagaaa acggcaagaa gccgtgaaaa tattagaagg cgcatctctt
    11941 ccgtttcata gacttatggg tggtagaaat gaaaaaatga gtattcgtta cgcgagaaca
    12001 gatcatctct ctgaatacat agatctggaa aatttcttaa aggatctgga agagtccaga
    12061 agaaaggaca tagcccaagg atctacaagt attggtcccc atagagacga tttaatattt
    12121 gcaattgata atatggaggc ctcaaagtac gcctctagag gccagtcaag aacggctatt
    12181 ttagctttaa agatggctga atcagaattt ttatatgccc gtaggactac aaaacctgtg
    12241 ttgttgctgg acgatgtaat gtccgagtta gaccaaacac gcagagaaaa gttagtagaa
    12301 cacctagaaa agcacgatca agttattcta actaccgctg aaccgagtaa cattccggaa
    12361 tcatttgtaa atagttccaa cagattggaa gttttaaacg gttccgtaaa aacaatttag
    12421 aattgaatgg aaatggaaat taaaaaacaa aaatcaatat ctgctgctgc caaaataata
    12481 gttcaatctc gatataccat agccttgatt ggtgccggat tatcagttga aagtggaata
    12541 cctactttta gaggtgccgg cggattatgg acaaaattag gtgaaccacc caacaacgga
    12601 tacgaaaatt ttcttgccga ccctaaagca tggtgggaac aaaacctaaa ttctgaagtt
    12661 gatcctgaaa gaactcaatt tcgagaggca attgaaaaag caaaaccaaa ccctggccac
    12721 ttagcattgg taaatcttga aaaacttaat attttaaaac accaaattac tcaaaatata
    12781 gacaacttac atcacattgc cggatccaag aatgtgaccg aaatacacgg aaatagaatc
    12841 aaattacgat gtgtcaattg tgatatgaga tggcatcgag atgagtttga ccaaataaga
    12901 ttggattgga aacaaaacct acctcccaaa tgtaatacat gcttaggaat cgtgaaacca
    12961 gatactgtaa tgtttggtga acccattccg ttttcgacct taaatacttg tgtaaatgaa
    13021 actagactgg ccgactgcat tttggtcatt gggacttcag caacagtata cccagccgct
    13081 ggtttcccaa gggaagtttc atcttccggt ggaaaaataa tagaaattaa cccggaggag
    13141 actccgatat ctgaagcggc taccgagtct ataaaaggcc ccacagaaga ttctttgcca
    13201 aaattagtag ccgagataaa gggaattatt ggcgatgacc ctgcttcata aatcaacaat
    13261 cctcgctggg ttttcacaca ttgcagccat gctagcaggt ctattattgc tctttttccc
    13321 agtcattagt gacttcgagc aaataacaga ttctggcaat ttcacccaac aattccagac
    13381 aaataaaact atctttgaag cgttgggtcc acaaggtctt ttcgtcataa tattaccttg
    13441 ggttttatcc ggagtatgta ttttttcttc aataatggct aaggctgcca acaataatca
    13501 caaaaccttg attcttagat ggaaatctta ctcgtgggcc gtgtcaatca tttttgtagt
    13561 gtttatcctt ataagtattt catccgttgg aacgttttat attccatcag gtctttttgc
    13621 gatagcttct tctttttata atcgatgagg agtgcaaagt gaagtacgac aagattgtta
    13681 taggcgccgg ctctgccggg gcagttctgg catctcgttt gtcagaggat cctgattgtt
    13741 ccgttctcct attggaagct gggccagatt atccagattt tggaacctta ccctttgatt
    13801 taaaacatgg atggggaact ggacctgaca tagttgtagg tggtattcat gattggcaat
    13861 ttaccggtac agcttccaca ttatcaaaaa ccatgcccat tcctcgtgga aaggtaacag
    13921 gaggtactag cgccataaat ggtcaggttt ttctacgtcc actaccagag gattttgaaa
    13981 gatgggttga atggggcaat gacaggtgga gttttaaaga atgcctgcct tacctcctta
    14041 aaattgaaac agacctagat tatgacggcg actttcacgg tcaagatggg cctattttag
    14101 taaaaagaca tcctcttaat tccttaactg atgaccaatt ggctttctat aaagcttgta
    14161 aatatttagg ctacgaggaa aatatagatc acaacctacc ggacgcaacg ggtgtcggac
    14221 catatcctct taacaaccct gacggcataa gattcagtac tgcgcttggt tacctttctc
    14281 aaagtagaca tcgcctgaat ttaactatta agccaaattg tgtcgcccaa aaaatcgtat
    14341 ttgatcgaaa tgtagcaacc ggtgttgaag tgatcagtgg tggtgaaatt ttcaaagttg
    14401 aaggaacaga ggtaatctta tcagcgggtt caatagcaac acctcaaatt ttaatgctat
    14461 ccggcattgg atcagaaaaa ttattgtcgc cattccacat tagtgtagta agtgatctac
    14521 ccggtgtagg aaaaaatctt cgagatcacc caacagttca cactacatgg acaccccatt
    14581 cttcattcaa caaaccaaaa caaaaaatag gtccgcaaaa agtcgccttg cggtacacct
    14641 cccaagattc caaataccaa aacgacatga tcatgattat gcgttttagt catgaaatag
    14701 gtgatgtcgg tgcgctcatg atctctgtag gaatatatct agctgaaagt gtgggcagaa
    14761 tttcacttca atccaccgat attcatacac aacccatcct agactacaac ctgttaagcc
    14821 ataaatatga tcttaaaaga ttaagagaag gggttaggct cgccgacaaa atagccaagg
    14881 cacccagttt caccgacacc atagttgcga gactttctcc agatgagatc accataaaca
    14941 acgacgactt acttgatcgg tggatgctgg aaaacgtttt gactatgcat cacatttcgg
    15001 gtacctgcaa aatgggtcca aagtcagatg cggaagcggt tgtagaccaa tatggtgtcg
    15061 tatatggaac tgaacaattg aggattgctg atgcttccat attgccagat tgtcctagag
    15121 caaacactaa cgttgcggca atgttgatag gagaaagaat agctgatttt ataaaaactg
    15181 gcgattgatg atgattttta cattaaatgc ggtatctaat cattatataa atgaatactg
    15241 gcgaatttta gacaccacta cttctctcat ctta
//
LOCUS       DASCNS010000265         1910 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001970734, whole genome shotgun sequence.
ACCESSION   DASCNS010000265 DASCNS010000000
VERSION     DASCNS010000265.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001970734"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="QF838_09510"
     CDS             <1..723
                     /locus_tag="QF838_09510"
                     /inference="COORDINATES: protein motif:HMM:NF023431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HJO60954.1"
                     /translation="QHEKILGKIEKEAEIIMWERDSTLYFFTIFGDPTGDIDPWGWRV
                     EGHHLSLHFSIWANDILSITPSFFGANPAKVQSGTHQGLEILAPRQSLALELMQSLDP
                     AQSKKALIFDEAPADILTFSSARASLPKEEGLKATNMSQTQKNILKNIVEEYIGEYRS
                     EIASGIVSKWTENIDTIHLAWGGGNQVGDPHYYRLHGGTFLIEYDNIQNGANHIHSVW
                     RDVDNDFAVDVLRDHLLLYHIT"
     gene            784..1320
                     /locus_tag="QF838_09515"
     CDS             784..1320
                     /locus_tag="QF838_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60955.1"
                     /translation="MSMSDGCALDFLAESMCIAIENMKSTIEQIGTTRSTNTLELETR
                     NWVTALFCYNSLENSNLISRIRKLGTHQTAMNLIEKSSDREIKLVQGIMDMLKEHNKN
                     GTLIQRIKNRFLLSITDLETEFIDHHSLVLEIKRSQNIVVPLSEITECEDTNCEWIFA
                     WLVETLGEEYQEYLVPYV"
     gene            1355..>1910
                     /locus_tag="QF838_09520"
     CDS             1355..>1910
                     /locus_tag="QF838_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015558.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HJO60956.1"
                     /translation="MNWFKPDPERISLGYGLLFCIALAVGAEALYFLEKSVFDFSLFD
                     SLVIVIVIAVLIRNLLPLPQKILPGSSFASKQILETAVLMLGASVNLFKIVDAGASML
                     LIIIFSVCGGLFIAWFVGHKILKLGSRLALLVGAGNSICGNSAVAAVAPAIGASSTEI
                     AAAISISAILGVVQILFLPLLAPTL"
BASE COUNT          583 a          363 c          426 g          538 t
ORIGIN      
        1 caacatgaaa aaatcctagg gaaaatcgaa aaagaagcgg aaatcataat gtgggaaagg
       61 gattcaacct tatatttctt caccattttt ggtgatccta cgggtgatat agatccctgg
      121 ggatggagag ttgaagggca tcatctttcg cttcatttca gtatctgggc caatgatatt
      181 ttatccataa ccccatcgtt ttttggagca aacccggcga aggtccaatc tggaacacac
      241 caagggcttg aaatcttagc acctcggcaa tcgttggcat tagaattgat gcagagtcta
      301 gatccggccc aatccaagaa agctttaatc tttgatgagg ccccagcaga tatcctaacc
      361 ttcagctcag ctagggcctc tcttcccaaa gaagagggct tgaaagcaac aaatatgagc
      421 caaacacaaa aaaacatcct aaaaaatatc gtcgaagaat acattggaga atatagatct
      481 gaaattgcga gcggcattgt atcaaaatgg acagaaaaca tagacacaat tcatctcgcc
      541 tggggtggag ggaatcaagt tggggacccg cattattata gattgcatgg gggaaccttt
      601 ctgatcgaat atgacaacat ccaaaatggc gcaaatcata tacattctgt gtggagagac
      661 gtcgacaacg actttgctgt ggacgtgttg agagaccacc tattgttgta tcacatcaca
      721 tgaactcaat aatcaaacct atagacttat gcttcactca aacaggaaaa caaccataac
      781 atcatgtcta tgagcgatgg gtgcgcattg gactttctgg ctgaatccat gtgtattgct
      841 attgagaaca tgaagtccac gatagaacag ataggaacga cccgcagtac caatacgctg
      901 gagctagaaa caagaaactg ggtaacagca ctgttttgtt ataactcttt ggagaacagt
      961 aacttaatat cacggatccg caaactggga acccaccaaa cagctatgaa tttgatcgaa
     1021 aagagttccg atcgagagat aaagttggtt cagggaataa tggatatgct gaaagagcat
     1081 aacaagaacg ggactttgat acaaaggatc aaaaatcgat tcctattatc cattaccgat
     1141 ctagagactg aattcataga ccatcattca ctagtgttag aaattaaacg ttcgcaaaac
     1201 attgtagttc cgctttctga aatcactgaa tgtgaagaca caaattgtga atggatattt
     1261 gcatggctgg ttgaaacgct tggtgaggaa tatcaagaat atttggttcc atacgtctag
     1321 aaacgaatat ggaatgttac ataaagaaag gttaatgaat tggtttaaac cggaccccga
     1381 aaggatctcg ttgggatatg ggttgctatt ttgtatagca cttgcagtag gtgcagaagc
     1441 actatatttt cttgagaaat ctgtgtttga tttttcctta tttgattctc tggtaattgt
     1501 aatagttatt gccgttctta ttcgaaattt acttccgcta ccacagaaaa tattgccggg
     1561 gtctagtttc gcctcaaagc agattttgga aactgccgtt ttgatgctag gggccagtgt
     1621 aaatcttttt aagatcgtgg atgccggggc ttcgatgctt ttgataatta ttttttcagt
     1681 ctgtggcggt ttattcattg cctggtttgt gggacataag attttaaagc tagggtctag
     1741 actggcatta ttggttggag ccggtaattc catttgtggg aattcggcag ttgcagctgt
     1801 ggctcctgcc attggggcat cttcaacaga aatagccgct gccattagca ttagtgctat
     1861 tctgggtgta gttcagatct tattcttacc gctattggca ccaactctaa
//
LOCUS       DASCNS010000266         8597 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001975464, whole genome shotgun sequence.
ACCESSION   DASCNS010000266 DASCNS010000000
VERSION     DASCNS010000266.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001975464"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="QF838_09525"
     CDS             complement(<1..764)
                     /locus_tag="QF838_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60957.1"
                     /translation="MGTLLKDKVAVVTGSGRGIGRGIALLLAEEGAKVVVNDLGGAVD
                     GSGDSVAPADEVVNEIKAAGGEAVANYDSVSEMDGGENIIKSALDSFGKLDIVVAVAG
                     ILRDRMLFNMTEEEWDAVIAVHLKGTFTVAKAASILFRQQRSGRIINFSSTSGLYGNS
                     GQANYGAAKDGIAGFTRAIARDLGRYGVTVNAISPGANSRMTQTVPQSARDIRASSGI
                     QANSAVTPLELNRDPEDVAPMVAWLASDEAKDVNGHV"
     gene            complement(816..1736)
                     /locus_tag="QF838_09530"
     CDS             complement(816..1736)
                     /locus_tag="QF838_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO60958.1"
                     /translation="MANYLDGKVAIVTGSGRGIGAGVAKQLASEGAKVIVNDIGAALD
                     GEGSSNTPAEQVVEEIKAAGGEATPNYTDISTMEGGEAIVKSALDNYDKLDIVVTVAG
                     ILRDRMIFNMTEQEWDDVIRVHLKGTFTVCKYASILYRQQRSGRIITFASESGLIGNT
                     GQGNYAAAKSGIAGFTKVAAKDLGRYGVTANSISPRANTRMTQSVPDSARQLRADRPD
                     DREAAPPTTQMEAEDVAPFVAYLASDQAASINGQTFLVYGGAITKLSLPRRVRTIFKQ
                     GRWDVEELRELAPQHLTQGLVNPSPPQAPK"
     gene            1860..2393
                     /locus_tag="QF838_09535"
     CDS             1860..2393
                     /locus_tag="QF838_09535"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HJO60959.1"
                     /translation="MLLNTLNEKKIGTTVHIVLYRPEIPYNTGNIIRLCANVGAELHL
                     IHPLGFELTESKLRRAALDYSDLAVVTEHENLEDYKNMYPDRIFYATSARSRKYYSEI
                     EFGSKDTYIFGPESSGIPPAVLDTIPRERHLLIPMKPGNRSLNIANAVSIIVYEAWRQ
                     NNFENAGPPRTISVSEN"
     gene            2562..2972
                     /locus_tag="QF838_09540"
     CDS             2562..2972
                     /locus_tag="QF838_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HJO60960.1"
                     /translation="MKRIQQSHIEEMIDHAKEHDPNECCGILAGTGEDITHIYRVKNS
                     TPSPFRYVMDPKEQFEVMKNADDNGLELLCFYHSHTHSPAFPSDTDVRMAVESGWVDF
                     GYALVSLEEKDRPKVNFFIIDSEGTVIPEDCEVV"
     gene            complement(2969..3580)
                     /locus_tag="QF838_09545"
     CDS             complement(2969..3580)
                     /locus_tag="QF838_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HJO60961.1"
                     /translation="MLIGLITDTHLPGRVRTLDELGDLPGEFLSSVDLILHGGDLTSP
                     IVLDWCEQFAPVICSTGNNDPIPDPRMKEVQVLEIDGWSIGMIHSLEGQFRPIHDLQK
                     LFPKPVQIMISGHTHQERLEYRDGVLLINSGSITFPQHKELRLGTVGTLEIYPGKAKV
                     EIFPLGETFGSPNPGKKMSMSIEDGIVTHSEGPVMLAQHNQQD"
     gene            complement(3621..4655)
                     /locus_tag="QF838_09550"
     CDS             complement(3621..4655)
                     /locus_tag="QF838_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014566.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HJO60962.1"
                     /translation="MDETIRETSKICLNCRNWISSSVASYCQNCGAYIKSETLSFLDN
                     SSTGKNNWWIWIVFTVIFIIVWQVIGSLPLVGFCWLLKIVKVAGYSCNLDELVITGPS
                     KAPNFLFMHTTFLIGAASFYFFLKILHNKNLLKVLTERIQFDYKRSLFGLNTFFLFAV
                     IPLATTLLTNTSELKLRGDVPDLLLITLIALIFVPIQAGLEEAFFRGYLLQGFSLLFK
                     SRVLILIITSSLFAFLHLMNPEPWSYGVGTYLISVFLVGMFMGLITLIDGGIELAVGI
                     HIANNLWVHLIVGLEDSVIPSSSLFITPNQNVDMIPTIIFSMSQYALLTFVFAFKYKW
                     FNKLKKYTLS"
     gene            4787..5977
                     /locus_tag="QF838_09555"
     CDS             4787..5977
                     /locus_tag="QF838_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119704.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HJO60963.1"
                     /translation="MAQTREAAIVGIHEYPLRVAPGVSALQIKAASASKALEDAGLSW
                     QDVDAVYDTGSHQNIGGLGISEYFGLNPRLIDNTSVGGTSYEVQAHHAATMIASGKAN
                     VALLTYGSTAHSDRRAIGTAGATGQGSPTPFNNMEDPWGMTLISGYGMVKARHMHQYG
                     TTNEQFAEISVATRYHAMRNPEAVEAMTALEFVGVNEITTQDVLDSRMIAWPLHLLEC
                     CMVSDGGGAVVIASKEVAKNCKKSPVWIIGGAEATKYRENGGDITVSAGAQSGPRAFE
                     DAGVSPNEIDVLMAYDSFSITVMCMIEDLGFCKKGEGGDYVSQGILKFDQKGGPALNT
                     DGGGLSSNHPGERGIFLLLEATRQMRGESTSQVDGAKLAVAVGNGGQLGARHATSPTI
                     LAAD"
     gene            5998..6420
                     /locus_tag="QF838_09560"
     CDS             5998..6420
                     /locus_tag="QF838_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013920.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HJO60964.1"
                     /translation="MTLRPQPRFPEPDTEPFWEATKEKNLVYQTCDNPDCTDKVIFYP
                     RAHCDTCGGLKTSWNTSKGEGVVYTYSVVMQSRHPAFKDLGPYAVAYVDLDEGFRMMT
                     NVINVDNPISDIQIGMKVKLVWQEQGEGEISLPMFEPA"
     gene            6509..7918
                     /locus_tag="QF838_09565"
     CDS             6509..7918
                     /locus_tag="QF838_09565"
                     /inference="COORDINATES: protein motif:HMM:NF018682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HJO60965.1"
                     /translation="MQHHPVPLKATLDSLPPLWPQDVLPQIRTILTETPSMVFVLDDD
                     PTGAQTVQDASFLTEWTRDMLLSEYHQPEKATFLLTNTRAKTEAEAVEINTTLGREFD
                     QLANYLKIPISIISRSDSTLRGHFPAELHALAKGMGNSHDGIVFIPFFIQGGRYTIDD
                     IQYVAGEGMLAPVGQTPFARDSAFGYESSNLRDWIEEKTFGKVLAKDVLSVSIDDIRI
                     GGPDVVAEKLKDMKSSDICIINAADMRDLDVVALAMLTLQNIYGKNYLIRSSASFVRS
                     RLGQEERPILTKKEMGYTRKGGALILVGSHVPGSTSQLESLLNLPNVYGLELDVHKVL
                     QHDKEQIISITEQTDRLLAESKDIVIYTSREVVTGSSPDEYLEIGTTITEAITEVVSD
                     ITITPRYIISKGGMTSSNIIVNALKIKRATVLGQIASGVLVLQLGLESKYPGSKLILW
                     PGNVGGPTAISDVVLRMRT"
     gene            7927..>8597
                     /locus_tag="QF838_09570"
     CDS             7927..>8597
                     /locus_tag="QF838_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HJO60966.1"
                     /translation="MFATSSDILKWADSQHVAIGGFNVYNFEGIKAVIEGAEEEETPI
                     IIQMHPASLQRGAKPLMAMALEAANLSKVPVSIHLDHVDSLEVVKEGIDFGLQSFMPD
                     GSIHNYQGNVEFTRLCARIIKDNEGFVEGELGRLSGTEDGTIIENYKSLLTDPLQVID
                     FVSSTTIDALAVCVGNVHGKYLSEPKLDFQRLYRIREATEIPLVMHGASGLPNEIISE
                     CIRAG"
BASE COUNT         2490 a         1724 c         1915 g         2468 t
ORIGIN      
        1 acatgaccat ttacatcctt ggcttcatct gaggccagcc atgctaccat gggagcaaca
       61 tcttcagggt cacggtttaa ctccaaagga gtcactgcag agttcgcctg aattccgctt
      121 gaagctctga tatccctggc agattgaggg actgtttgtg tcatcctaga atttgcccca
      181 ggggatatag cattgacagt tacaccatat ctgcctaggt cacgtgcgat agctctggta
      241 aatcctgcta taccatcctt cgcagctccg taatttgctt ggccggaatt gccgtacaaa
      301 cctgaagtgg aagagaaatt aattattctg ccactgcgct gttgtctgaa cagtattgaa
      361 gcagctttag ctacagtgaa ggttcctttc agatgaacag caataactgc atcccactct
      421 tcctctgtca tgttgaacag cattcgatct ctaagaattc cagccacggc tacaacgata
      481 tctagtttgc cgaagctgtc tagagctgat ttgataatat tttccccacc gtccatctcg
      541 gagacagaat cgtagttagc gactgcttca cctccggctg ctttaatttc atttacaact
      601 tcgtcggctg gggcgacaga atcgccgctg ccatctacgg caccaccaag gtcgtttacc
      661 actacctttg cgccctcttc cgcgagtaat aaggctatac cacgccctat accgcgacct
      721 gaaccggtta ctactgctac tttatcctta agaagggttc ccatctttct tactcctgac
      781 ttttttactc ttaataccta tctgaaccat taacactatt tgggtgcttg tggtggtgat
      841 gggttgacta agccttgtgt gagatgctgt ggagctagtt ctctaagttc ctctacatcc
      901 caccttcctt gtttgaatat ggtgcgaact cggcgtggca gggaaagctt agtaattgct
      961 cctccatata cgaggaaggt ctgcccattg atgctagctg cctgatcaga agccaaatat
     1021 gctacgaatg gtgcgacatc ctccgcttcc atctgagttg ttggtggagc tgcttcccta
     1081 tcatcgggcc gatcagctct taattgtcta gccgaatcgg gaacagactg agtcattcta
     1141 gtgtttgctc ttggagaaat agaatttgct gttaccccat atcggcctag gtcttttgca
     1201 gctaccttgg tgaaacctgc gattcccgat ttggcagctg catagttacc ttgtcccgta
     1261 ttccctatca aaccagactc tgatgcgaat gttattattc ggccacttct ttgctgacga
     1321 tacaagattg aggcatattt gcaaactgta aaagtgcctt ttaaatgaac ccgaataaca
     1381 tcatcccatt cctgttctgt catattgaaa atcattctgt ctcttaatat tccagctacc
     1441 gtcactacga tgtctaattt gtcatagttg tccaaagccg acttgacgat agcctctcca
     1501 ccttccattg tagatatatc agtgtagttc ggtgtggctt ctcctcctgc agcttttatc
     1561 tcttccacga cttgttctgc tggggtattt gaactgcctt ctccatctaa tgcagcaccg
     1621 atatcattta caatgacttt ggctccttcc gaagccaatt gtttcgctac tcctgcccct
     1681 atccctctac cagatcctgt gactattgca actttaccgt ctaggtaatt cgccattttt
     1741 ttcctcctga aattcgttcg caagacaaaa ttgaccttat cattagcgcg caacgttcat
     1801 tgtgccacaa atggacagat attgtcatag ttggtttagt ttatttattc tgaactaaaa
     1861 tgttattaaa cactctaaat gaaaaaaaga ttggtactac tgtgcatatt gttttataca
     1921 gacctgaaat tccgtataac accggtaata taattcggct ttgtgctaac gttggggctg
     1981 aattacacct gatccatccg ttgggatttg aactcacaga atctaaatta agaagggcgg
     2041 ctttggatta ttccgattta gcagttgtga cggagcatga aaacttggaa gattacaaaa
     2101 acatgtaccc tgatagaatc ttttatgcta catcagctcg ctcccgaaaa tattacagtg
     2161 aaatagaatt tggaagtaaa gacacataca tattcggtcc agaatcgtca ggaataccac
     2221 ctgcagtcct agatacaatt ccaagagaaa ggcatctgtt gattcctatg aaaccaggaa
     2281 ataggagcct gaatattgca aatgcggttt ccataatagt ttatgaggct tggagacaga
     2341 acaatttcga aaatgcgggt cctcctagaa caatttctgt atcagaaaat taaaatactg
     2401 aaaagaggag tagatttatg ggacgctcca attggcatta tgagcaccct ccggcgtgta
     2461 catgcgttaa atgtgttgaa gaaaggcaaa aaaatggttc tggcggaatt tttcagatca
     2521 taaaaaggtt aatattcttc agaagaaagt aggagaaaag tttgaagcgg atacagcaaa
     2581 gtcatattga agaaatgatt gatcatgcaa aagaacatga tcctaatgaa tgttgtggga
     2641 tattggctgg gaccggtgag gacataactc atatatatcg ggttaaaaac tcgacgccaa
     2701 gcccattcag atatgtaatg gacccaaaag aacaatttga agttatgaag aatgctgacg
     2761 ataacggtct tgaattattg tgcttttatc attcgcatac tcacagccca gcttttccat
     2821 cagataccga tgttaggatg gcggtcgaat ccggttgggt tgatttcggg tatgcactgg
     2881 tttcgttgga agaaaaagat agaccgaaag ttaatttttt tatcatcgac tctgaaggaa
     2941 ctgttatccc tgaagattgt gaagtagtct aatcttgttg gttatgctga gcgagcatca
     3001 ccggaccttc gctgtgggtg acaatcccgt cttctatcga cattgacatt tttttcccag
     3061 ggttcggact tccaaaggtt tcgcctaagg gaaatatttc tacctttgcc ttgccggggt
     3121 atatttcgag tgtcccgacg gttcctagcc gcaattcttt atgttgtggg aatgtgatac
     3181 tgccagaatt gattagaagc accccatctc tatattccag gcgttcttga tgagtgtgcc
     3241 cggaaatcat gatttgtacc ggctttggaa acaatttttg aaggtcatgg atgggccgaa
     3301 attgtccttc taaactatgg atcattccaa tggaccatcc gtctatctca agaacctgaa
     3361 cctctttcat ccgaggatca ggaattggat cattattgcc tgtagaacaa ataacaggag
     3421 cgaactgttc gcaccaatca agaacaattg gtgaagttaa atctccacca tgaagaatta
     3481 aatctacact agaaagaaat tctcctggaa gatcacctaa ttcatctaaa gttctgaccc
     3541 gtccgggtag atgagtgtcc gttattaatc caattaacat ccgaagaact atagtttcga
     3601 tttactgagt tatcaatagg ttatgagagg gtatactttt taagtttatt gaaccattta
     3661 tatttgaaag caaatacaaa tgttaacagt gcgtactgac tcattgagaa tatgatggta
     3721 ggaatcatgt ccacattttg atttggggtg atgaaaagag aagaacttgg tatcactgaa
     3781 tcttccaaac caactattaa atgtacccat aaattatttg caatatgtat accgacagct
     3841 aactctattc caccgtcaat taatgttatt aatcccataa acataccaac cagaaaaacc
     3901 gaaatgagat atgttccgac accgtatgac cacggttcag gattcattag atgcaagaaa
     3961 gcgaaaagtg atgaagtaat aattaagatc agtacacgtg atttaaacag caatgaaaag
     4021 ccctgtaata gatatcctct aaaaaacgct tcttctaaac ctgcctgtat tgggacaaat
     4081 attaaagcga tcaaagtaat tagcaataaa tctgggacat cgcccctgag tttcaattct
     4141 gaagtattag ttagaagtgt ggtcgccaag ggaatcactg cgaacaaaaa aaaggtgttt
     4201 agtccaaaca aacttctttt gtaatcgaat tgaatccgtt cagttaatac cttcaaaaga
     4261 tttttattgt gcaaaatttt gaggaagaaa taaaagcttg cagcaccaat taaaaaggtg
     4321 gtgtgcatga aaagaaaatt aggtgctttg gaaggacctg ttattactag ttcgtcaagg
     4381 ttacaactgt aaccagctac ttttacgatc tttaggagcc agcaaaatcc gactaaaggg
     4441 agcgagccaa ttacttgcca aactatgatg aatataactg taaaaactat ccatatccac
     4501 cagttatttt tgccggtgga ggagttatcc agaaaagata gcgtctcgga ttttatatat
     4561 gcaccacagt tttggcagta ggaggctacc gaagaggaaa tccagttcct acagttcaga
     4621 catatttttg aagtctctcg aatcgtttcg tccatattat gagggtatct gacaaatata
     4681 ttttggacaa taaaacttaa ttttcaatat ttcattgacg gcttagaagg catgtgctaa
     4741 cttcattttc attactaata gcacaaattt tttcgaggac taacttatgg ctcagacgag
     4801 ggaagctgca attgtaggaa tacacgaata cccattaaga gtggcgcctg gcgttagcgc
     4861 tttgcaaata aaggccgcat ctgcatccaa ggcattagag gatgccggtt tgtcatggca
     4921 ggatgtagac gctgtttatg acaccgggtc ccaccaaaat atcggaggac ttggtatttc
     4981 ggaatatttt ggcctgaatc caagattgat tgataacacg agcgttggcg gcacttcgta
     5041 tgaggttcaa gctcatcatg ctgctactat gatagcttct ggcaaggcta atgttgcctt
     5101 gttaacatat gggtccacag ctcattcgga tcgtcgagca ataggaactg ctggagcgac
     5161 tggtcaagga tccccaactc ccttcaataa catggaagac ccttggggaa tgacgttgat
     5221 atctggatac ggcatggtga aagcccgcca tatgcatcaa tatggcacca caaatgagca
     5281 gttcgcagag atttcggtgg ccactagata tcatgccatg cgcaatcctg aggctgtgga
     5341 agctatgaca gcccttgagt ttgttggggt taatgaaatc accacccagg atgttctaga
     5401 ttcaaggatg attgcttggc cgcttcatct tttggagtgt tgcatggtat cggatggtgg
     5461 tggagcagtt gtgatcgcgt ccaaagaagt tgcaaagaat tgcaaaaaat ctcctgtatg
     5521 gattatcggt ggtgccgaag ctacgaaata tagagagaat ggaggggata tcactgtaag
     5581 tgctggtgct caatcaggac ctagggcttt tgaagacgct ggagtatcac caaacgaaat
     5641 tgatgttttg atggcatacg attcattttc cataaccgta atgtgcatga ttgaagattt
     5701 aggattttgc aagaaaggtg aaggtggaga ttatgtcagt caagggatac tgaagtttga
     5761 tcaaaaaggg ggccccgctc ttaacacaga tgggggaggg ctatcttcaa atcacccggg
     5821 tgagaggggc attttccttt tgcttgaagc gaccagacag atgagaggag aatcaacttc
     5881 ccaagtggat ggtgccaaac ttgctgttgc cgttgggaat ggtggtcaat tgggagctag
     5941 gcatgcaacc tcaccaacca ttcttgccgc ggattagtaa atcaggagaa ttataaaatg
     6001 acattgagac ctcagcctag atttccagaa cctgacaccg agccattttg ggaagcgacc
     6061 aaggaaaaaa atcttgttta ccaaacatgt gataatccag attgcaccga taaggtgatt
     6121 ttttacccta gagcccactg cgacacctgc ggtggtttaa agaccagttg gaacacctca
     6181 aaaggcgaag gtgttgtgta cacatacagt gttgtaatgc agagtcgaca tccggccttc
     6241 aaagatttag ggccatatgc tgtggcctat gtagatctag atgaaggctt taggatgatg
     6301 accaatgtta taaatgtgga taacccaatt agtgatatac agattggtat gaaggttaaa
     6361 ttagtttggc aggaacaagg tgaaggagag atctcacttc caatgtttga gcctgcctaa
     6421 attttagctg ctcttgaaaa aggttaatca cgaattcata tcagccttct tgaagtatta
     6481 gcttaagcga atttaagaag ggggatttat gcagcatcac ccagtgccac ttaaagcgac
     6541 tctggattcc ttacctccac tttggcctca ggatgtgcta cctcaaataa gaacaatatt
     6601 aactgaaact ccatctatgg ttttcgtttt ggatgacgac ccgacggggg ctcaaactgt
     6661 ccaagatgcc tcatttttaa ctgaatggac tcgtgacatg ctactttccg agtatcatca
     6721 acctgagaag gcaaccttcc tgctgacaaa cacaagggca aaaacggaag cggaggcagt
     6781 ggagataaat accacactcg gtagggaatt tgaccaacta gcaaattact tgaagatacc
     6841 catctcaata atatccaggt cagattcgac tctccgtgga cactttcctg cagaattgca
     6901 tgctttggcg aagggaatgg ggaactccca tgatggtatt gtctttatcc ctttttttat
     6961 tcaaggggga cgttacacga ttgatgatat ccaatacgtc gcaggggaag gcatgctggc
     7021 gccggtagga caaacgccct ttgctagaga ttctgcattt ggttacgaat catcaaattt
     7081 acgtgattgg atagaagaaa aaacttttgg gaaagttctg gctaaagatg tattgagtgt
     7141 ttcgatagac gatatcagaa ttggtggtcc agatgtggtg gcagaaaaac taaaagatat
     7201 gaaatccagc gatatatgca taattaatgc agcagatatg agagatttgg atgtagtggc
     7261 tctggcaatg ttgactttgc aaaatatcta tgggaaaaat tatttaatac gttcttctgc
     7321 ttcgtttgtt agatctcgac ttgggcaaga ggaacgacct atccttacca agaaagaaat
     7381 gggatacaca agaaaagggg gggcgctcat tctcgttggt tctcatgtgc cgggtagtac
     7441 ttctcagcta gagagtctcc taaatctacc gaatgtatat ggtttggagt tggacgtaca
     7501 taaagttctt caacatgaca aagagcaaat aatttcgata actgaacaaa ccgatagact
     7561 tttggcagaa tcgaaggata ttgttattta tacgagtcga gaagtcgtaa ccggatcgtc
     7621 tcctgatgaa tatttagaaa tagggactac tatcaccgag gcgataacag aagtcgtttc
     7681 tgatatcaca atcaccccaa ggtacattat atctaaaggc ggtatgacat cctcaaatat
     7741 cattgttaat gccttgaaaa taaaaagggc cactgttctt gggcaaattg cttctggagt
     7801 tttggtatta cagttgggtt tggaatccaa atacccagga tctaaattaa ttctatggcc
     7861 tggaaatgta ggcgggccta cagctatttc agatgtggta ttgaggatga ggacttagag
     7921 tatcagatgt ttgcaacctc ttcagatatt ttgaaatggg cagattctca gcatgtcgcg
     7981 ataggcggct ttaatgttta caattttgag ggaataaagg ctgttataga aggggccgaa
     8041 gaagaggaaa ctccgataat tattcagatg catccggcat cgcttcagag gggagctaag
     8101 cctctaatgg ctatggcatt ggaggcagcc aacctatcca aagtaccagt ttctattcat
     8161 cttgatcatg tagatagttt ggaagttgtt aaggagggta ttgatttcgg tcttcagtct
     8221 ttcatgcccg atggatcaat acataactat caaggaaacg tggaatttac ccggttatgt
     8281 gctcgaataa ttaaggataa tgaaggtttt gtagagggag aattaggccg gctttcaggt
     8341 actgaggatg gtacaatcat tgaaaattac aagtcacttt tgacagatcc gttgcaagtg
     8401 atcgattttg tgtccagtac tacgattgat gcccttgcgg tctgtgttgg taatgtgcat
     8461 gggaaatacc tctctgaacc aaagttggat tttcagcggt tgtacagaat acgtgaagct
     8521 acagagatcc cgctcgtaat gcatggtgcg tcaggtctac cgaatgaaat tatttcagaa
     8581 tgtatacgtg ccgggat
//
LOCUS       DASCNS010000267         1802 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001984727, whole genome shotgun sequence.
ACCESSION   DASCNS010000267 DASCNS010000000
VERSION     DASCNS010000267.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001984727"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="QF838_09575"
     CDS             complement(<1..232)
                     /locus_tag="QF838_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO60967.1"
                     /translation="MGYTFTDWVFNKPVTSRNSKLNFEENPMGLLDGKKVLITGARKG
                     IGRGIAITLANEGADVGVNDILDDEFVKKLLEI"
     gene            444..1340
                     /locus_tag="QF838_09580"
     CDS             444..1340
                     /locus_tag="QF838_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HJO60968.1"
                     /translation="MPNRDFQGYANSPPKVVWPNNSRIAISVVVNYEEGSEYSIMDGD
                     ETHESNNEVPSPVPSSQRDLANESFFEYGSRVGVWRILDILDGYSIPASFFCCALALE
                     RNLIVGPEIIRRGHEVVGHGYRWEEYYLMDPESERTAIDKTVESLEKTTGVRPQGWYT
                     RYAPSEKTRELIADTEGFIYDSNSYSDDIPYYENVNGKPWLIVPYSLEVNDTRFWRGG
                     MNRPSDFFETLKNTFDLLYEEGLNNPKLMNIGLHCRIVGRPSRAGVLKEFFEYANSKP
                     DVWFARRDEIAKWWLKNYPPNS"
     gene            complement(1383..>1802)
                     /locus_tag="QF838_09585"
     CDS             complement(1383..>1802)
                     /locus_tag="QF838_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO60969.1"
                     /translation="TFAVYKYHSEKGTPHGKRRASELKTDHTKSLLAAMDQGVKIVAG
                     TDAGGWVHGNNAEELVCLVEAGMSPSQAILAGTQTAAECIGLQEELGTIEKGKKADLL
                     FVSQNPLEYISCLQFGETVSLVMKGGEVVSDKRHESL"
BASE COUNT          488 a          399 c          366 g          549 t
ORIGIN      
        1 ctatttccag cagcttttta acgaattcat catctaaaat atcatttacg ccaacatccg
       61 caccttcatt tgccaatgtt attgcgatgc cgcgtcctat tccttttcta gctccagtaa
      121 ttagaacttt tttaccatca agtaacccca ttggattctc ctcgaaattt agttttgaat
      181 tcctgctcgt cacgggcttg ttaaataccc aatcggtgaa agtgtaaccc atttgcagag
      241 caatgaaatt gcggctaggt gggtatggat gtataagtac agtaggattt cgagtccctt
      301 ctagaggccc cagtattttc tcctctacct agttagtttc accgaccaaa ttctatgcca
      361 tgagtggata atatctattt atgattttgt tttagaatgc tgtcatgaat agtcaattta
      421 tcaaatgaag tgagaagaat catatgccca acagagattt tcaaggttat gctaattcac
      481 ctcccaaagt cgtttggccg aacaattcac gtatagctat ctcggtagtt gtgaattacg
      541 aagaaggttc tgaatattca attatggatg gtgatgaaac ccatgagtcc aacaatgagg
      601 tgccctcacc tgtaccttct tcgcaaagag atctcgcaaa tgaatctttc ttcgaatatg
      661 gaagccgggt tggagtatgg aggatcctgg atatactgga cggctattcc attcctgctt
      721 cttttttctg ctgcgccttg gcacttgaaa gaaacttgat agtcggccct gagataatta
      781 ggcgtgggca tgaagtggta ggccatggat atagatggga agagtactac cttatggacc
      841 cagaatccga aagaaccgct attgacaaaa ccgtggaatc ccttgaaaaa accaccggtg
      901 tcagacctca aggttggtac acgagatacg ctcctagcga aaaaactagg gagctaatcg
      961 ctgatacgga aggattcatt tatgactcga attcttattc tgacgatatc ccttattacg
     1021 aaaatgttaa tggtaaaccc tggttgatcg tcccgtattc tcttgaagtg aatgatacgc
     1081 gtttttggcg aggtggaatg aatagaccat ccgatttttt cgagacacta aaaaacacgt
     1141 ttgatctttt gtatgaagaa ggccttaaca atcctaaact tatgaatatt ggccttcatt
     1201 gccgtatcgt tggtcggccc tctcgggctg gggtcttaaa agaatttttc gaatatgcta
     1261 actctaaacc tgatgtatgg tttgcgagaa gagatgagat tgcgaaatgg tggcttaaga
     1321 actacccacc aaattcgtaa aaatatcttg gcacgattct cttaagtcaa tttgatgcgc
     1381 tgctacaagc tttcatgccg tttgtcagac accacttccc cccccttcat tactagggag
     1441 acagtttcac cgaattggag acaagaaata tattcaagag gattttgaga tacaaataaa
     1501 agatccgctt tttttccttt ttctatggtc cctaattctt cctgaaggcc aatgcattcc
     1561 gctgccgtct gggtcccggc tagaatagcc tgactagggc tcattccagc ctccactaaa
     1621 cagaccaatt cttctgcgtt gtttccatgg acccaacctc ctgcgtccgt accggccact
     1681 attttcaccc cttgatccat cgctgccaac aagctcttag tatgatcagt ttttagttca
     1741 cttgctctac gtttcccgtg aggggtacct ttttcagaat gatatttgta tacagcaaat
     1801 gt
//
LOCUS       DASCNS010000268         2123 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001988756, whole genome shotgun sequence.
ACCESSION   DASCNS010000268 DASCNS010000000
VERSION     DASCNS010000268.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001988756"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="QF838_09590"
     CDS             <1..321
                     /locus_tag="QF838_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar accessory protein FlaH"
                     /protein_id="HJO60970.1"
                     /translation="LTTFVSRAGGEQIQDFFNEMKNVCEKGQVIACTVHENAFDEDFL
                     LRVRSICDAYLRFQVSAHGTNLVKTMEVAKIRGAEMRTGNILGFEVEPGMGIRIVPIS
                     RAKA"
     gene            335..>2123
                     /locus_tag="QF838_09595"
     CDS             335..>2123
                     /locus_tag="QF838_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HJO60971.1"
                     /translation="MAFNLKNLKVIVKMGKADGSEDQNGSMNGNVVSLQDGIKGFLQN
                     TGVGHSSEFVLDEKLLARLPAEFRDQAEQFRHLGAYVSKLPIDDIGIPEYLGFVSRGS
                     DIQKKRNLVYPVGGGVFIHVVHDPEDTRDYYLAVEPALAPGVEEIVDDIDVQLMDYID
                     ELRSADGAEGRLNVILSAVDEICGRANGSSKKVSKPINLTDDQFQAIKYLMRREKAGM
                     GVMDPLVEDPYIEDISCSGVGPLYIEHKVFGGLKASIEFSDSEELDQYVIQLSEKIGR
                     PVTIREPIVDATLPDGSRINIVYGGDVSRRGSNFTIRKFSATPLSILDLIKFGTLTYE
                     MAAYISLMLSEGMNTFVSGETASGKTTILNAISTFIPPNAKIVSIEDTPELQVPHQNW
                     IREVVRGGGENSASVTMFDLLVAALRQRPEEIVIGEIRGAEGAIAFQAMQTGHACMAT
                     FHAASVEKLIQRLTGNPINVPKTYIDNLNMVVIMSAVRLPDGSPGRRCLSISEIIEYD
                     PESNSFGFIEVFKWNPAIDEFEFPGYMNTYLLEQVVASRRGLPPDEYRNIYGELERRA
                     EVLKRLSDRDTTNFYELHNVLAQANREGLF"
BASE COUNT          635 a          387 c          530 g          571 t
ORIGIN      
        1 cttacaacat ttgtttcgag ggccggggga gagcagatac aggatttttt taacgaaatg
       61 aaaaatgtct gtgagaaagg ccaagttata gcatgtacag tccacgaaaa tgcttttgat
      121 gaagattttc ttttgagagt aagatcgatc tgtgatgctt atttaaggtt tcaggtcagc
      181 gcacacggaa ctaatttggt taaaactatg gaagttgcaa aaattcgtgg tgcggagatg
      241 cgaaccggta atattttagg gtttgaagtt gaacctggta tgggaattcg tatagttcct
      301 atttcaagag ctaaggcata ggttgaggca ctaaatggcg ttcaatctca aaaatcttaa
      361 agtaatcgtc aaaatgggaa aggctgacgg ttccgaagac caaaacggct cgatgaatgg
      421 caatgtggtt tccctgcaag atggaattaa gggatttctt caaaacaccg gggtaggtca
      481 ttcttcagag tttgtcctag atgaaaaatt gcttgcgaga ctgccggctg aatttagaga
      541 ccaagcagaa caatttagac atttgggagc atacgtttcc aaacttccca ttgatgatat
      601 aggaatacct gaatatctgg gatttgttag cagaggatct gatattcaaa agaaacgaaa
      661 tttggtttac ccggtcggtg gtggagtgtt cattcatgtg gttcacgatc cggaggatac
      721 ccgggattat taccttgctg ttgagcccgc tcttgctcca ggagtagaag aaatagtcga
      781 tgatatagat gttcaattga tggactacat cgatgagctt cgatcagctg acggggccga
      841 agggcgcctt aatgtcattt tgagtgccgt agatgaaatt tgcggtaggg ccaacgggtc
      901 ttccaagaag gtttcaaaac cgatcaattt gaccgacgac caatttcagg cgattaaata
      961 tttaatgcgt agagagaaag ctggaatggg agttatggac ccgttagtag aagaccctta
     1021 cattgaagat ataagttgta gcggtgtagg ccccctttat atagagcaca aagtatttgg
     1081 aggattgaaa gcaagcattg aattttctga ttctgaggaa ttggatcaat acgtaataca
     1141 gctttcagag aagatcggaa gaccagttac aatccgggag cccatcgtag atgcgaccct
     1201 gccagacggc tcgagaataa atattgtcta cggaggtgat gtctcccgtc gggggagtaa
     1261 ctttacaatc agaaagttta gtgcaacccc acttagtatc ctagatctta ttaaatttgg
     1321 aaccctgaca tatgaaatgg cagcttatat ctctttgatg ctgagcgagg gtatgaacac
     1381 cttcgtgtca ggtgagacag cttcagggaa aacaacgatc cttaacgcca taagtacatt
     1441 tataccccca aatgcgaaga tagtgagcat tgaggatact ccggagcttc aggtaccaca
     1501 tcaaaattgg ataagagaag ttgtaagagg aggtggtgag aatagtgcct cggttactat
     1561 gtttgattta ctcgttgctg ccctaaggca gagacctgaa gagatcgtca tcggtgagat
     1621 caggggcgct gagggggcca tagcctttca ggcaatgcag accggccacg cctgtatggc
     1681 tacatttcac gctgcttctg tagaaaaatt aattcaaagg ctgactggaa acccgatcaa
     1741 cgttccgaag acttacattg ataatttaaa catggtagtg attatgagtg cggttagact
     1801 tcctgatggt agcccgggta gaaggtgtct cagcataagt gaaattatcg aatatgatcc
     1861 agagagcaac tctttcggat tcatagaagt tttcaaatgg aaccctgcta tcgatgaatt
     1921 tgaatttcct ggttacatga acacatatct tctagagcag gtggttgctt ctcgcagagg
     1981 attacctccg gatgaatata gaaacatata cggcgaactt gagagaagag cagaagtttt
     2041 aaaacgtctt tcagataggg acacaacaaa tttttatgaa ctccacaacg tcctcgcgca
     2101 agcaaataga gaaggtctct tct
//
LOCUS       DASCNS010000269         1916 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001992266, whole genome shotgun sequence.
ACCESSION   DASCNS010000269 DASCNS010000000
VERSION     DASCNS010000269.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001992266"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /gene="rpmE"
                     /locus_tag="QF838_09600"
     CDS             <1..155
                     /gene="rpmE"
                     /locus_tag="QF838_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HJO60972.1"
                     /translation="SCGETFITGATKPELRVEICSSCHPFFTGEQRIVDTEGRVERLR
                     RRYNLQ"
     gene            166..1089
                     /locus_tag="QF838_09605"
     CDS             166..1089
                     /locus_tag="QF838_09605"
                     /inference="COORDINATES: protein motif:HMM:NF018798.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HJO60973.1"
                     /translation="MISERVRNFSPFFFDTGKVAPRYGGQAVLEGIMIRGQNNVAIAV
                     RSPSGEIVTKVKKINTLFTGKLRSIPIIRGILALAETLSLGMDALNYSSQVANQEEDT
                     PPSKFSSVMMILVSLSIAVGIFFILPLLVSKPFEGILGSDVISNVAEGIIRLLVFLVY
                     VTAISLMPDIRRVYMYHGAEHMAVHAQEKGKPLTIEEIRKYPTAHPRCGTAFLLTIMV
                     IAIVVFTFIPRDPLWMVIGSRIVLIPLIASLSYEFIRFNSKYETNKWVSKLSIPNLLL
                     QSLTTKIPDDDQIEVAISAMKLAISTDAKSM"
     gene            complement(1093..1728)
                     /gene="hisIE"
                     /locus_tag="QF838_09610"
     CDS             complement(1093..1728)
                     /gene="hisIE"
                     /locus_tag="QF838_09610"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017472093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HJO60974.1"
                     /translation="MSTIQFDEKGLIPAVVQDHQTRKLLMVAYMNRESLAKTLESKEA
                     WFYSRSRENLWHKGETSGNFLIVRSIKLDCDGDTLLLEVDPKGPACHTGAESCFNTDL
                     SDLDNCNFIGADTDLNVFRDLFSIIEDRKLNPKNASYTNKLFDEGVNRIAQKVVEEAG
                     ETAIAGVTGQKEELASEISDLIYHVFVLISANNMSLEQVFAELSKRRESGN"
BASE COUNT          566 a          412 c          375 g          563 t
ORIGIN      
        1 gctcttgtgg tgaaaccttc attacgggag cgactaaacc tgaactaaga gtagaaatat
       61 gctcttcctg tcacccattt tttaccggtg agcaaagaat agtagacaca gaaggccgag
      121 ttgagcggct gaggcgtcgg tacaacctac aatgagaggt aattcttgat tagtgaaagg
      181 gtgagaaatt tctcaccctt tttttttgac acgggtaagg ttgcccccag atatggtggt
      241 caagctgtcc ttgaaggaat aatgatacgg ggccagaata acgttgcaat tgctgtaaga
      301 tctccttcag gtgaaatcgt aacgaaagtc aaaaaaatca atactctttt cacaggaaaa
      361 ctaagatcta tcccgatcat tagagggatt ctggcattgg cagaaacttt atcattagga
      421 atggatgcat taaattactc gtcgcaggtt gccaaccaag aagaggacac ccctccaagt
      481 aaattttcat ccgtaatgat gattttggtt tctttgagta tagctgtcgg aattttcttc
      541 atattgccat tattggtgag caagccattt gaaggcattt taggatctga cgttataagc
      601 aacgtagccg aaggcattat caggctgctt gttttccttg tatatgtaac tgctattagt
      661 ctaatgccag atattagaag agtttatatg tatcacggtg ccgaacatat ggcagtccat
      721 gcgcaggaaa aaggcaaacc cttaaccatc gaggagattc gaaaataccc cacagcgcac
      781 ccaaggtgtg gaacggcatt cttgctcact attatggtga tagccatagt ggtctttact
      841 tttataccga gggatccatt gtggatggtg attggatcac gaatcgtttt gattccttta
      901 atagctagtt taagctacga atttattcga tttaacagta agtatgaaac caataaatgg
      961 gtctctaaac tatcaatccc taatttacta ctgcaatccc taacaacaaa aattccagac
     1021 gatgatcaga ttgaagttgc catcagcgct atgaaattag ccatatctac agatgccaag
     1081 tctatgtagg tattaattac cagattctct tctcttagac aattcagcga acacctgctc
     1141 gagagacata ttgttggccg aaataaggac aaaaacgtgg tagatgagat cagaaatttc
     1201 tgaggccaat tcttcttttt ggccagtcac tcctgctata gcggtttccc ctgcctcctc
     1261 taccaccttc tgggcaatac gattaacccc ttcatcaaac aatttgttag tgtagctagc
     1321 atttttggga tttaacttac gatcctcgat aatggaaaat aaatccctga aaacattcaa
     1381 gtcagtatca gcacctatga aattgcaatt atctagatct gataaatcgg tattgaagca
     1441 actttccgca cccgtatggc aagccgggcc cttaggatca acttctagca acaaggtgtc
     1501 tccatcgcaa tcaagcttta ttgacctgac gatcaaaaag ttccctgaag tttccccttt
     1561 atgccagagg ttttccctgc tcctactata aaaccaagcc tccttactct ctaatgtctt
     1621 agcaagtgat tctcgattca tataggcaac catcaatagt ttacgtgttt gatggtcttg
     1681 gacgactgcc gggattaaac ctttttcatc aaactgtata gtagacatgt aatcaccctt
     1741 accgttttca ttccacacta tttaacgcat gggtcttata ggaatattac gatctaacaa
     1801 gtattctttt gcctgcgaaa tagaaaaagt tccaaaatgg aatatactgg cagctaaaac
     1861 cgcatctgct cgacccactt caatagcctg tctcaaatgt tctagattcc ctgcgc
//
LOCUS       DASCNS010000270         3583 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000001995395, whole genome shotgun sequence.
ACCESSION   DASCNS010000270 DASCNS010000000
VERSION     DASCNS010000270.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001995395"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="QF838_09615"
     CDS             complement(<1..630)
                     /locus_tag="QF838_09615"
                     /inference="COORDINATES: protein motif:HMM:NF020390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HJO60975.1"
                     /translation="MSEKENRRMLAHLMRRAGFGATSKELDGLSKMSYASVVDTLLDP
                     PDRSWMGEHLIRRFHHEQSGMMSAFGPSEYWLYQMVTTKAPLVEKMTLFWHGIFATGY
                     PKVIHGKVLSNQIAMFRKHGLSELHELLIELSKDPAMIVWLDNQENHNGAINENYGRE
                     LLELFSMGVGNYSEDDIKEAARAFTGWSIGNTEYMVLRSDRDSDMPYGRI"
     gene            complement(658..2214)
                     /locus_tag="QF838_09620"
     CDS             complement(658..2214)
                     /locus_tag="QF838_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HJO60976.1"
                     /translation="MKYDFIIVGGGSAGSVLATRLSEDPNKSVLLLEAGPDYRTFEDT
                     PNVVKYGNIPWPASYGPEAPVWGYKATAVSGRVPFGLPRGKIIGGSSSVNGQVFFRGI
                     PEDYDEWEASGNEGWSYLDVLKYFRKSENDLTFGSDDFHGNEGPIPVRRYPDNELMPS
                     SKAFLQACISQGYEFTEDQNHPESTGVGMKPLNNVDGVRMSVALTYLSMARHRLNLTI
                     RGEVLVRQVRFDGTKAIGVQVESQGDVFDLDAEEVILCGGAINSPQLLMLSGIGPSKH
                     LKSIGIDLRKHLPGVGQNLRDHPSAFLLFESYIDQVGEDPKAQQVGMRFTVPQSKVRN
                     DMQVSPLFMTSEHRPDTIPLDPEKNYLGFSVALQKALSSGEITLKSSDPHEQPNLFYN
                     YLEHPEDLRRMREAVRICVTITQNETFNPIIKSRIDSTGFDLECDASLDKWLYSIVGT
                     QHHSSGTCKMGPKEDPLAVVTQTGLVHGIDNLRITDASIMPDVVRANTNATVIMMAEK
                     IADDIFNANK"
     gene            complement(2275..2637)
                     /locus_tag="QF838_09625"
     CDS             complement(2275..2637)
                     /locus_tag="QF838_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJO60977.1"
                     /translation="MYFKQTEAGDEKKAQRIHEGDGLIERRNLFGSSSNLPIQIEIWE
                     LEEGVSEGDHIHGDACPLEEIYYFLEGSGEMTIEGERVLVEKNDALMDPPGLDHGIRN
                     TGTGLLKMMIVWGISSYK"
     gene            complement(2747..>3583)
                     /locus_tag="QF838_09630"
     CDS             complement(2747..>3583)
                     /locus_tag="QF838_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60978.1"
                     /translation="ALAKDRESHSQWQRAISYLILIAGLLYICEALSNVIPLWVGYKY
                     LSYLCLLGSVIFPVFLLLKPGVDSKSYRKGEILLNFGLFITLVLIFTSNLSITIYSLT
                     NPRNDTLGRIGFDQMPVGFGGGSPDSKVTPEIERARLRGTVITAIVRTEPYALTAAIE
                     YIYKGKGSGKYLMGTDSYNTAAKVAYHAAEYSPHIPRLPILPIYSEYQDTWITSLEDL
                     EKITTHGELKYILTSKDMKSMNFEFWTWLSGNFEDVTILSGLPFNGELRVFKLLERDV
                     RN"
BASE COUNT          975 a          838 c          680 g         1090 t
ORIGIN      
        1 tattcttcca taaggcatgt cggaatcacg gtctgatcgt aaaaccatat actccgtatt
       61 tcctatactc cagccagtga aagccctcgc agcctccttt atgtcatctt cggaataatt
      121 accaactccc atggaaaata gttccaacaa ttccctaccg taattttcat tgatcgcccc
      181 attatgattt tcctggttgt ctaaccatac gatcattgca ggatcttttg ataattcaat
      241 caataattca tgaagctcgc taagcccgtg ctttctgaac attgctattt gattggataa
      301 aactttccca tgaataactt taggatatcc agtggcaaaa ataccgtgcc aaaacaaggt
      361 cattttctct actagtggag cctttgttgt caccatttga taaagccaat attcactagg
      421 accaaaagca ctcatcatcc ctgattgttc gtggtggaat cttctgatga gatgttcccc
      481 catccaagat ctatcaggtg ggtccaataa agtgtccaca acagacgcat atgacatttt
      541 tgacaaaccg tctaactcct tcgaagtggc tccaaagcca gccctcctca taagatgcgc
      601 aagcatccgt ctgttttcct tttcactcat gaaaacactc cttttccgga aatgtaccta
      661 tttgttggca ttgaatatat catccgcgat tttttcggcc atcattatta ctgttgcgtt
      721 ggtatttgcc cttactacat caggcattat tgatgcatcg gtaattctta aattatcaat
      781 gccgtggact aaccctgttt gggtcactac ggctaaagga tcttcctttg ggcccatttt
      841 acaagttcct gaggagtgat gctgagtgcc aactatcgaa tatagccatt tatctagact
      901 ggcgtcacat tccaagtcaa aaccggtgga atcaattctc gatttgataa ttgggttaaa
      961 ggtttcattt tgcgtaattg tgacacaaat tcttacggcc tctcgcattc ttcgtaggtc
     1021 ctctgggtgt tctaaatagt tgtaaaaaag atttggttgt tcatgagggt cggaagattt
     1081 caaggttatt tccccactgc ttaatgcttt ttggagagcg acactgaacc ctaggtaatt
     1141 tttttctgga tctagaggta tggtatctgg acggtgctca ctagtcatga acagagggct
     1201 cacctgcata tcattcctta ccttagattg aggcactgtg aatctcatcc ctacctgttg
     1261 tgctttgggg tcctccccaa cttgatctat atatgactca aagagcaaaa aggcagatgg
     1321 atggtctctc aggttttggc caacaccagg aaggtgtttc cttaagtcta tgcctattga
     1381 cttcagatgt ttgctcggcc cgatgccaga caacattagt agttgtggtg aatttatcgc
     1441 acccccacaa agaattactt cttcagcatc caagtcaaaa acatctcctt ggctctcgac
     1501 ttgtacccca atggccttgg taccatcaaa ccttacctgt cggaccaaaa cctcccctcg
     1561 tatagttagg ttcaaacgat ggcgagccat cgaaagataa gtcaacgcaa cgctcatccg
     1621 aacaccatct acgttattta gcggtttcat accgactcca gtggattcag gatgattttg
     1681 gtcttcagta aactcgtaac cttggcttat acaagcttga agaaaagctt ttgaggaagg
     1741 cataagctca ttatctggat atctgcgtac tggaattggt ccttcattcc cgtgaaagtc
     1801 gtcagaccca aaggttagat cattttcaga tttcctaaaa tatttgagga catccaaata
     1861 agaccatcct tcgttgcccg atgcttccca ttcgtcataa tcttcaggaa tcccacgaaa
     1921 gaatacctgt ccgtttacgg aactagagcc gccaatgatt tttcctctag gtaacccaaa
     1981 agggacacgg cctgatactg ctgtcgcttt atatccccat accggagcct ccggaccata
     2041 ggaggctggc cacggaatgt tcccatattt aactacattc ggggtgtctt caaaagtacg
     2101 ataatcaggt cctgcttcaa gtaacaaaac tgacttattg gggtcctcag acaagcgtgt
     2161 ggccaagact gaacctgccg atccgccacc aacaattatg aagtcatatt tcatattaaa
     2221 cctcttttca tatccaatac agatttatct cccgcagtaa ggttaattat gaccttattt
     2281 atatgatgat ataccccaaa caatcatcat tttcaagagg ccggtgccag tatttctaat
     2341 gccatggtca aggcctggtg gatccatcaa tgcatcgttt ttttcaacca aaacccgttc
     2401 tccttcgatt gtcatctccc cactaccttc cagaaaataa tagatttcct ctaatggaca
     2461 ggcgtcccca tgaatatgat ctccctcact gactccttct tctaattccc agatttcaat
     2521 ctgaatgggc aagttgctag aagatccaaa aagatttcta cgctctatga gcccgtctcc
     2581 ttcatgtatg cgctgagcct ttttttcgtc acccgcttcg gtttgtttaa agtacatgta
     2641 aaatccttat tttaaacctt gttgcctttt cggctttatt aaaatatctc ggctatctta
     2701 ccgatccttc ggctcattat ttttgaatcc tttgttttca ggatgttcaa tttcggacat
     2761 ctctttcgag caatttaaaa acacgaagtt caccattaaa gggtaatccc gatagaatcg
     2821 ttacatcttc aaaattaccg gacaaccaag tccagaattc aaaattcata ctcttcatgt
     2881 ctttcgaagt caggatatat ttcaattccc cgtgggtagt tattttttcc aggtcttcca
     2941 aagaggtgat ccaagtgtct tgatattccg agtaaatcgg tagaattggc aaacgtggta
     3001 tatgtggtga atattctgcc gcgtggtagg caaccttcgc tgcagtatta taggaatcgg
     3061 tacccataag atatttacct gatccttttc ctttatatat atattctatg gctgctgtta
     3121 aggcataagg ttcagttcgg acaatcgcag taatcaccgt tccacgaagg cgagcccgtt
     3181 ctatttcggg agttactttg gagtcaggtg agcctcctcc aaagcctacc ggcatttgat
     3241 caaagccaat cctacctaac gtatcatttc gcggattagt aaggctgtag atagtaatag
     3301 aaagattgga cgtaaaaatg agtactaagg tgataaataa accgaaattt aaaagtattt
     3361 ctcctttgcg ataagatttt gaatcaaccc ctggtttcaa aagtaagaag acaggaaaaa
     3421 taactgaacc aagcaggcac aggtagctga ggtatttgta tccgacccac aggggaatta
     3481 cattggataa ggcttcgcaa atgtacagga gcccggctat cagtatcaag tatgaaatag
     3541 cacgctgcca ctgtgagtga ctctctcggt ctttcgccaa tgc
//
LOCUS       DASCNS010000271         2060 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002008776, whole genome shotgun sequence.
ACCESSION   DASCNS010000271 DASCNS010000000
VERSION     DASCNS010000271.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002008776"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="QF838_09635"
     CDS             <1..222
                     /locus_tag="QF838_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60979.1"
                     /translation="VGVGVGVGVGGSGVGVGPGVLVGGGTGVTVGRAVAFGCACGVSC
                     SSFFTFDSVSDIEHETAHNEITRIEIPVR"
     gene            822..1958
                     /locus_tag="QF838_09640"
     CDS             822..1958
                     /locus_tag="QF838_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HJO60980.1"
                     /translation="MWNRMILTMRMLCVVVMVMLIALLSVSCTSDPEIIEVTRIVENV
                     VEVVKEVEVIKEIPVASDANGHHQVDAKPLVIYSGRSESLVDPLIEDFSELTGIEVQV
                     NYAKTGPLAATLLEEGDKTPADIFFAQDPGGLGAVDALLTELPNDLLIAVPEWARDPE
                     NKWVGTSGRARVIVYNTEELTEADLPDSIEDFVKPEWKGRIGWPPTNSSLHAMITGMR
                     QEWGEERTRTWLEGIVANEPKIYEKNTPTVKAAGAGEISVGFVNHYYLHRLSAEEGES
                     FAARNYYTRAADPGSVVLVAGVGMLEASDNKDAAAQFIKFLLSASGQQFFAAKTYEFP
                     LNGEAKPNNLLPPLEEITKPSIDLGDLTDLQGTQDLLREVGALP"
BASE COUNT          576 a          369 c          500 g          615 t
ORIGIN      
        1 gttggcgttg gtgtcggtgt cggagttggt ggcagcggag ttggtgtagg ccccggagtg
       61 ctagttggcg gtggaactgg cgtgactgta gggcgagcag tagcttttgg ttgtgcttgc
      121 ggagtctcgt gctcatcgtt ttttacattc gattctgttt ctgacatcga gcatgagacg
      181 gcccacaacg aaattacaag aattgaaata ccggtcaggt agatccgaat tgaactgctc
      241 aagaacttac tctcatctat taggtgtaaa aattttcacc gagatggtcg ggagcgtgac
      301 catatctgaa aaacgccaca gttttttaga ttcctataca actgtacaaa ttgtttgaac
      361 aagtagcttt ataaaaatac taggctgcat tatttttttt cactgcatta agccgtatat
      421 atcctgttta gcccgcaaga atcgtagagg aaaagtagag cttttcttac gtaaatggga
      481 ttgtatttgt cgagagggtt ctaaaggatt tgaatccgac acttatttta ctgaattcga
      541 tagatcttca ttgaataaag tgatggtgta aaacgataat ttaacgatac ttctttttat
      601 acgaacaaaa taatttgtac tgacatggta atttgggttt ctgtaagttg agaataaaat
      661 tatgctataa catcactaaa ttcatttagt aaaagctgtt gtatgcggtg tataattgtg
      721 tattatctag gtcaggattt ctatatacga aaaaaataca tgtacagacg cttacccggc
      781 ggcaagtctt tactgtttag atatattgga gagaaattaa catgtggaat cgaatgattc
      841 tcaccatgag aatgttgtgt gtcgtcgtta tggttatgtt gatagccttg ctttcggttt
      901 cttgtacatc cgatccagaa atcattgaag tgacacgtat cgtggaaaat gtggttgagg
      961 ttgtaaaaga agttgaagta atcaaagaga tccctgtggc gtctgatgcg aatggtcacc
     1021 atcaggttga tgcaaaacca ctagtaatat attcagggcg tagtgaatca ctggttgacc
     1081 cattgattga agatttttcc gaattaaccg gtattgaagt tcaggttaac tatgccaaaa
     1141 caggtcctct tgcagccact cttcttgaag aaggtgacaa aaccccagca gatatattct
     1201 ttgcacaaga tccgggtgga cttggtgcag tagacgcctt attaactgaa ttacctaatg
     1261 acctattaat tgctgtgcct gaatgggcgc gtgatccgga aaataagtgg gttggaacct
     1321 caggtcgagc ccgtgtaatt gtttacaaca cagaggagtt gactgaagca gatcttccag
     1381 attctattga agattttgtc aagcctgaat ggaaaggtcg tataggctgg cccccaacga
     1441 acagttcttt gcatgccatg atcacaggta tgcggcaaga atggggtgaa gaacggactc
     1501 gtacttggtt ggaaggtatc gtcgcaaatg agccaaagat ttacgagaaa aatactccga
     1561 ccgtcaaggc agctggagcg ggtgaaattt ccgttggttt tgtaaatcat tactatcttc
     1621 atcgcttgag tgcggaagaa ggggaatcat ttgctgcccg caactattac acgcgagctg
     1681 ccgaccctgg ttctgtcgta ttagtagctg gggtaggtat gcttgaagct tctgacaata
     1741 aagatgcggc tgcacaattt atcaagtttt tgcttagtgc atccgggcag cagttttttg
     1801 ccgctaaaac gtacgaattt cctttgaatg gcgaggctaa gccaaacaat ttgctgccac
     1861 ctctggagga aattactaag ccaagcattg atttgggtga cttaactgat cttcaaggga
     1921 cacaagatct ccttagagag gttggtgctt tgccttgatg aggctggcca tttttgactg
     1981 caggtaatta gtgattccat cgcagaaatc acatactttc gaagaagatt aacattcatc
     2041 gctgaatagc aggaagttgg
//
LOCUS       DASCNS010000272         8031 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002010308, whole genome shotgun sequence.
ACCESSION   DASCNS010000272 DASCNS010000000
VERSION     DASCNS010000272.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002010308"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="QF838_09645"
     CDS             complement(<1..290)
                     /locus_tag="QF838_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60981.1"
                     /translation="MNASTLRGKATAVQMLVDTLAKITDLLKIPLRTNSGRIGLAILT
                     VYSIVVIFGPWLTPYIPTEYDLDHRFESPSLNHFFGTDHNGRDVLSRVIAGA"
     gene            complement(292..1239)
                     /locus_tag="QF838_09650"
     CDS             complement(292..1239)
                     /locus_tag="QF838_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60982.1"
                     /translation="MLRFLVIRLAFIAVTLFFVSVAIFTATEFLPGDAASLLLGQQAT
                     ESNLKNLRHNMGLDRHPVERYAVWIKNAVRGDLGMAARGGATINSMVKSRLPNSALLA
                     AVAFLIAVPTSLLAGVLAGIRPDTKLDRILSIGSLLAISIPEFIIGVILMMVFATKLG
                     WFPSSSILLPGETILGTPKILVLPTLTITGALFAYILRMARANVIEVMESNYVRTAIL
                     KGLSMRKVVFKHVLPNAMIPTITVIANNVGWMFGGIIVVEFVFAYPGIGSLLIMAIDT
                     RDMRLLQSTALVIAAIYAFANLAADLLYGLLNPRVRVGQ"
     gene            complement(1364..3079)
                     /locus_tag="QF838_09655"
     CDS             complement(1364..3079)
                     /locus_tag="QF838_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60983.1"
                     /translation="MLRSILGNRTANSFMILSIAAFSIFMTACQGETIIKEVEVIKEV
                     EVEVEVEKEIIKEVIKEVKVEVEKEVIVVATPMPVITGAGGQQYGGTLKLGIIDFGAM
                     DPSIMGLSEGSAIYSNHTYDNLTEPWYDGGIVNRLAETWSANDDLSVYTINVRQGVTF
                     HDGSALTSADVKWTFDRIMDETTASPLLGEIDYISEITTPDAATAIFTLKGSNTNLPQ
                     DISDYHARIVPDGITQETLQESSTDFGTGPYTLGDHNPAERTVMNKYDSYWVEGKPFY
                     DRIIMYYMPEEATRIEAIKSGAVDVIGSFSLAQVEGLNERDSITVTGTDSATIRNLVL
                     DTREESIFSDKNARKALQYAIDRDFVRKAVTYGFGANANDHPIGTADQMYWDEQPLSN
                     QDIELSRSYLTAAGYNDSNPLSFELDASDFNQMLEMALAVEQSVEATDLPIEIEVTKH
                     EESTFWEAIWMQPGGTPAVTSAWNGRPAAQAVSVALKGGGSWNESYFDNPRMDELLEL
                     SSTEIDFDKRKAYWKEIQEILIEEVPSVYLLYVPVLVAHRTHVQGVQAHPNAWTFMED
                     WWSSE"
     gene            complement(3450..5114)
                     /gene="ggt"
                     /locus_tag="QF838_09660"
     CDS             complement(3450..5114)
                     /gene="ggt"
                     /locus_tag="QF838_09660"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJO60984.1"
                     /translation="MFSTYKQEVVGSKGVVASNHPLASLAGVEMLTSGGNAVDAAIAT
                     IFALSVVEPMMVGITGAGFINYYNSRDKSSITIDNYCTAPIKATEDMFTPISDKWPDY
                     METKNHQNRYGYLSVGVPAALQAWAHIEEKYGLLGIEKVISPAIRFSKEGFRVSDYLS
                     GIVENNKDILSSYPSSKSIFLGNGVALKTGDTLANPDYAKTLEEIAKNGVSTLTEGKL
                     GNSIERDMKSNKGIVSLDDLDQYKIKYREPVRGTYRGYEIVGVGPVSSGATHIIQALN
                     IIEGFDVKEMQFGSVEYIHLIAETLKLVFADRFKYMGDPDFVKVPVEGLISKEYGQHC
                     RQKIDLSKSSSFEHDNPAAFATESKNTTHITVADETGSVVSMTQTLNDAFGSRVTVPG
                     TGVLLNNTMYNFDPHPGTANSIAPGKRVLSSMAPITVFKSGKPFMSLGTPGARRIFPS
                     VLQGIINVIDHGMSLQEAVEAPRVWTQGQNLELEPDISPDVIEPLTQKGHVIEGVERV
                     AGGMNGVLFDDTGTIHGAACWRADGSPIAVGGGPATIKGSNPMFRV"
     gene            complement(5121..5681)
                     /locus_tag="QF838_09665"
     CDS             complement(5121..5681)
                     /locus_tag="QF838_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJO60985.1"
                     /translation="MSPARMMYELSNAVPKNSIIVDDSISNRPAMRHYFPAVERNDIR
                     GVRGQSIGGGIGATMGTKCAFPDRPVFGIIGDGSAMMTVQGLWTAANDKIPCIFVICN
                     NGMYRVLKTNFNVYQKEILNEPEPAGDNLLYSDFITPFNICTIAEGMGLHSERVTDPN
                     EIAGAVDRALASGAPALLDIVIDGKI"
     gene            complement(5712..6782)
                     /locus_tag="QF838_09670"
     CDS             complement(5712..6782)
                     /locus_tag="QF838_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60986.1"
                     /translation="MSQTMSGKQAVLEMLKAEGIEYIFGNPGTSEGPLIDMLGDYPEF
                     KYIMALQESVAMGMGESFARSTEKTSFVSLHVDSGLANGIALMLDALNTGTPMVVTSA
                     NYDSRKINETKTDLAELVRPVTKWAVELDLPDQIPSVLRRAFNEANSHPKGPVYVGFT
                     ANALEGIADMNIVPSGKVHDDVIPSPSGILEAAKLLTHAKNPALIVGDRVSDDDAINE
                     SISIAELLGMAVFQSRGAEVSFPTTHPQYQGMHSLRSQASRESLKEFDVILTVGMDTL
                     DELFYWGDVILDESQKLIHIDPIPGRVGRSEPTDVGIISNCKTGLTNLIAEVSKTVDQ
                     NIVSTRKSQVISDASSQKGDYE"
     gene            6829..>8031
                     /locus_tag="QF838_09675"
     CDS             6829..>8031
                     /locus_tag="QF838_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO60987.1"
                     /translation="MTPITAIALTHANMPVLMFIKEKFTGLRKMAEIAAGQAVIELLR
                     AEGVEYVFGVTGLTTNSMVTQMYGREDIKFIDTRHEEGAILMAYGYSKSTGKPSVCMT
                     TSGPGTINLAGGMSLALKGRAPVIAIAGDTAMEYIGRDGAQAFDLVNVFKPLTKMAVQ
                     ANKTERVPGLLREAFRSAMWGKQGPVLLDIPRDLLDKETLSDEMLNPDQYRTVNPRVS
                     GDLDAVQQAVGLLLSAKRPLLLAGGGVVDSEASNQAVQLADLLDMAMVPSYGHHDAIP
                     NSHPNYIGPPGGRGSGEAHEAMDRADVILALGTKINQASSSWDYSVINQETKIIQVDI
                     DPLEIGRNYPVSVGIVGDAQRVAQQLIASISVIAADGKTNDSWKSEFQSIANKRLQRL
                     ESEKSLNAN"
BASE COUNT         2135 a         1764 c         1766 g         2366 t
ORIGIN      
        1 ctggcgccgg ctatgaccct acttaaaaca tctcgaccat tatggtcagt accaaaaaag
       61 tgatttaggg acggagattc aaatcgatgg tccaaatcgt attctgtcgg aatataaggc
      121 gtaagccatg ggccaaaaat cactacgatg gaataaacgg ttaaaatcgc caaccctatt
      181 cggccgctgt ttgtacgaag tggtattttc agaaggtcag tgattttcgc aagagtatct
      241 accaacattt gaacggccgt tgcctttcct ctcaaagtac tagcattcat tttattgtcc
      301 tactctaact ctaggattca agaggccgta aagtaggtcc gccgcgaggt tcgcgaatgc
      361 ataaatagcg gctatgacta gagctgtaga ttgaagcaat cgcatatctc gggtatcgat
      421 ggccataatc agtaggcttc caatacctgg gtatgcaaaa acgaattcaa caacaatgat
      481 tcccccaaac atccatccga cattgtttgc tattactgta atagtgggga tcatggcatt
      541 cggtaagacg tgcttaaaca caacctttct catactgagg ccttttaata tggcagtcct
      601 gacgtaattg ctttccataa cctctatcac gttggctcga gccatgcgca atatataagc
      661 aaataaagcc ccagtgatag taagggttgg aagcactaat attttggggg tacccagaat
      721 agtttctcca ggaagcaaaa tactggagga cggaaaccaa ccaagtttgg ttgcaaaaac
      781 catcattaaa atgacaccaa taataaattc tggtatcgaa attgcaagga gactccctat
      841 gctgagtatc ctgtcgagtt ttgtgtctgg tctaatgccc gccaagactc ctgcaagcaa
      901 tgaggttgga accgctataa ggaatgctac cgctgcgagt agagcagagt tcggaagtct
      961 acttttaacc atgctgttaa tggtagcacc gcctcgagcc gccattccca aatcgcctcg
     1021 tacagcattt ttgatccaca cggcatacct ttctactgga tgccggtcaa gtcccatgtt
     1081 atggcgcagg tttttcaggt tgctctctgt ggcttgttgg cctaaaagga gagaagcggc
     1141 gtctcccggg agaaactcgg tagctgtaaa gatagcaaca gaaacaaaga aaagagtaac
     1201 agcgataaaa gctagcctta tgacgaggaa tcttaggata ttatgaaact ccttggcctg
     1261 aggttaaatg gggcgggtca cgtcagcacg tgatctagga ttgtttcata cctagatcac
     1321 ccactgacgc attcaacgtg ttacgttttg gatattggtg tacctattcg gaactccacc
     1381 aatcttccat aaaggtccag gcattaggat gagcttgcac tccttgtacg tgggttcgat
     1441 gtgcgactag aactgggaca taaagaaggt aaacactagg aacctcttct ataaggattt
     1501 cctgtatttc tttccagtaa gcttttcttt tgtcaaaatc aatttcagta gaggacagct
     1561 ccaataattc gtccatccta ggattatcga aatacgactc gttccaactt ccaccaccct
     1621 ttagggccac gctaaccgcc tgggctgctg gacgtccgtt ccatgcggaa gtaacagcgg
     1681 gtgttcctcc gggctgcatc catatagctt cccagaatgt ggattcttca tgctttgtaa
     1741 cttctatctc aatgggtaga tcggtggctt caacagactg ttctaccgcg agtgccatct
     1801 ctaacatttg gttgaagtct gatgcatcaa gctcaaagct taatgggtta gagtcgttgt
     1861 atccagcagc tgtaagatag ctgcgggaga gttcaatgtc ttgattgctg agaggttgtt
     1921 catcccagta catttggtct gcagtaccta ttggatggtc gttagcatta gcaccaaatc
     1981 catacgttac tgcttttctt acaaaatctc gatcaatagc gtattgcaga gcctttctag
     2041 catttttatc gctgaaaata ctctcttcac gagtatccaa gaccaagttt ctgattgtgg
     2101 cagagtcggt tccggtgact gtgattgaat ctcgctcatt gagaccttcc acctgtgcca
     2161 aagagaaact gccgattaca tcgacggcgc cagattttat agcctctatt ctcgttgctt
     2221 cttcaggcat gtagtacatg ataatccggt cgtagaaagg tttcccttcg acccaataac
     2281 tatcatattt attcattacg gttctttctg ctgggttatg atcaccaagt gtatagggac
     2341 ctgttccaaa gtcagtgctt gactcctgga gtgtctcctg ggttatgccg tcaggaacaa
     2401 tacgtgcatg atagtcagat atatcttgag gtagattggt gttggagcct ttaagcgtga
     2461 atatcgctgt agcagcatca ggagttgtta tctcactgat gtagtctatt tcccctagta
     2521 aaggggacgc ggttgtttca tccataatcc ggtcaaaggt ccactttaca tcagccgatg
     2581 ttagagcgga tccgtcgtgg aatgtgactc cttgtctaac attgatagtg taaacagaca
     2641 gatcgtcgtt agctgaccat gtttcggcta gtctgtttac tatcccaccg tcgtaccatg
     2701 gttcggtgag gttgtcatat gtatggttac tgtatatggc actaccttcc gatagtccca
     2761 taatagatgg gtccatagca ccgaagtcta taattcctaa cttcaaggta ccaccatatt
     2821 gctggccgcc tgcccctgtt ataacgggca ttggagtagc cactactatt acttctttct
     2881 caacttcaac cttaacttcc ttgatgacct cttttattat ttctttttca acttcaactt
     2941 caacttcaac ctcttttatg acctccacct ctttgataat agtctcgcct tggcaggctg
     3001 tcataaatat agaaaaagct gcgattgata gaatcatgaa cgagttcgcg gttctattgc
     3061 ctaaaatact tctcaacata tagctctcct tagttttgtg gaaaatcgcg aattcttaag
     3121 tttataaata tttctacacc ctgtctcaag acccaattag gtaaagtttt ataccacgac
     3181 atagaaggtt gaagcaccgg gttgttgatt gttcagctca tgatcgatga acttggtgct
     3241 acaccattgc ggtgttaaga attaatgcca ttcaaggtcc gccttgaatg cttagcagta
     3301 aggtataaac ctcacgaata tgcggttgtt tgaagttatc tccctactcc gggtttgtgg
     3361 atgatccttc cgaagaggta ttaaatatat ccaaatactc ggcaatggtc aagttattga
     3421 agccaaattt aaggtaaatc gcaaagcaat tagactctaa acattgggtt gcttcccttt
     3481 atagttgctg gacctcctcc aactgctatc ggtgacccat cagccctcca gcaagcagca
     3541 ccgtggatag ttccggtgtc gtcgaataaa accccattca tcccacctgc aactctttcc
     3601 accccttcaa taacatgccc tttttgggtt aatggctcta tcacatcagg ggaaatgtct
     3661 ggttccagtt caagattttg accctgtgtc caaactctgg gggcttctac ggcttcttgt
     3721 agcgacatac catgatcaat aacgtttatt atcccttgta gtaccgatgg gaatatgcgt
     3781 cttgcccccg gtgttcctaa ggacatgaaa ggtttacctg acttaaatac agtgattggt
     3841 gccatgctgc tcaagacacg tttcccaggt gctattgaat tggccgttcc ggggtgagga
     3901 tcgaagttat acatagtatt gtttaacaaa acccctgtcc ccggcaccgt aacacgtgaa
     3961 ccgaaggcgt cattaagggt ttgggtcatg gacaccacac tgccggtttc atctgccacc
     4021 gtaatgtggg tggtgttttt tgattcagtg gcgaaagccg ctgggttatc gtgttcaaaa
     4081 ctagatgatt tagacaaatc tattttttgc ctacagtgtt gcccatattc ttttgagata
     4141 agaccttcta caggcacttt gacaaaatca gggtctccca tgtatttaaa cctatcagcg
     4201 aaaaccaatt tgagggtttc cgcgattagg tgtatgtatt ccacactacc gaattgcatc
     4261 tcctttacat cgaaaccctc tattatattt agagcttgaa ttatatgggt agcaccagaa
     4321 cttaccgggc ccaccccgac aatttcatat cctcggtaag tacctcgcac gggttctctg
     4381 tatttgattt tgtattgatc taggtcatcc agtgatacga ttcctttgtt ggacttcatg
     4441 tctcgttcaa ttgaattacc taatttgcct tctgtcagag tggaaacgcc atttttggca
     4501 atctcttcca gtgtcttagc atagtcagga ttagcaagag tgtctcctgt tttaagagca
     4561 acgccatttc ctaagaaaat tgatttgctg ctaggatatg aagacaatat gtccttgttg
     4621 ttttctacta tcccagaaag ataatcactt accctaaatc cttcctttga gaatctaatt
     4681 gctgggctta taactttttc aatacccagg agtccatatt tctcttctat atgagcccaa
     4741 gcttgaaggg ccgcaggaac tcccactgat agatatccat acctgttttg atggtttttg
     4801 gtttccatat aatcaggcca cttatcagaa attggtgtga acatatcttc agtagctttt
     4861 ataggggcag tgcagtaatt gtcgatagta atggaagatt tatctcttga gttgtagtaa
     4921 ttgataaatc ctgctccagt tataccgacc atcataggct cgacgacaga aagtgcgaaa
     4981 atggtggcta ttgctgcgtc cactgcattt ccacctgagg tgagcatttc aaccccggca
     5041 agggatgcta aagggtggtt agacgcaaca actccctttg aaccgactac ttcttgctta
     5101 tatgtagaaa acatctgtga ctaaattttc ccatctatga caatatctag taatgcggga
     5161 gctcctgagg ctaaggctct gtcaacagcg ccggcgatct cgttcggatc ggtgacacgc
     5221 tcactgtgta ggcccattcc ttcagcaata gtacaaatat tgaatggagt gatgaaatca
     5281 gaataaagga gattgtctcc ggctggttca ggctcgttaa gaatttcctt ttggtaaaca
     5341 ttaaagttcg tcttgaggac cctgtacatt ccgttattgc agattacaaa tatgcaagga
     5401 attttgtcgt tcgcagccgt ccataggccc tgtacagtca tcatcgcgct gccatcacca
     5461 ataattccga acacaggcct gtcagggaaa gcacactttg tccccatagt ggctccaatg
     5521 ccgccaccaa tcgactgacc acggacccct ctaatatcat ttctttcgac ggctggaaaa
     5581 taatgtctca tagcgggtct gttacttatt gaatcgtcta ctattatcga attcttgggg
     5641 acagcatttg atagttcata catcattctt gcaggggaca ttggagtgct gttccatttt
     5701 tccttgactg attattcgta gtcacccttt tgagatgaag cgtctgatat tacctgagat
     5761 tttcgagtcg aaactatatt ttggtctact gtcttggaaa cttcggctat tagattggta
     5821 agaccagttt tacagtttga aattattcct acatctgtcg gttcagatct acccacccga
     5881 ccaggaatcg gatcaatgtg aattagtttc tgggattcat ccaagatgac atcaccccag
     5941 taaaataatt catctaatgt gtccattcct acggtgagga ttacgtcgaa ttcctttaaa
     6001 gattctcttg atgcttgtga tcttagagag tgcattcctt ggtattgcgg gtgtgtggta
     6061 ggaaaagata cttctgcccc tcgtgactga aacaccgcca ttcctaggag ttccgcaatg
     6121 cttattgatt catttatggc atcatcgtct gagaccctgt cacccacgat aagtgcggga
     6181 ttttttgcat gagttagtaa ttttgcggct tccaaaatgc ctgaggggct tggaattaca
     6241 tcgtcatgaa ctttgcctga agggacaatg ttcatatctg ctatgccttc caaagcgttc
     6301 gctgtgaaac caacataaac tggccctttt ggatggctgt ttgcttcgtt gaacgctcgt
     6361 cttaaaacgc ttggtatttg atcaggtaag tctaattcca ctgcccattt agtgacaggt
     6421 cgaacaagtt cagcgagatc agtcttggtc tcatttatct ttctcgagtc atagttagct
     6481 gaagtcacaa ccattggagt gccagtgttg agggcatcaa gcatcagagc gattccgtta
     6541 gcaagcccgc tgtccacatg gaggctgacg aatgaggtct tttctgtcga acgagcgaat
     6601 gattctccca tacccatggc tacactttcc tgtaatgcca tgatgtattt gaattctggg
     6661 tagtctccca gcatatcgat cagcggacct tcactagttc ctggattacc aaaaatatac
     6721 tcaatgcctt cagctttgag catttcaagt acagcttgtt ttccagacat ggtttgcgac
     6781 atctttgccc cctagaattt ctttttcttg agggaaatat ggtacagatt gacgccgata
     6841 acggcaattg ctctaactca tgctaatatg ccggtgttga tgtttatcaa ggaaaaattt
     6901 acaggattga gaaaaatggc agaaatcgca gcaggccagg cagtcataga attacttaga
     6961 gctgaagggg tcgagtatgt atttggagtt accggtttaa ccactaatag tatggtcacc
     7021 caaatgtatg gtcgtgagga tattaaattt atagatacca gacatgagga aggtgccata
     7081 ttgatggcat atggatattc caaatccacc ggaaaaccat cggtgtgtat gactacttct
     7141 ggccccggga ctataaatct ggctggtggg atgtctcttg cattgaaggg gagggcacct
     7201 gttattgcaa tcgctggtga cacagcgatg gaatacattg gcagagacgg tgctcaagca
     7261 ttcgatttag taaacgtttt taagccgttg accaaaatgg ctgttcaggc aaataaaaca
     7321 gaaagagttc caggtttgct tcgagaagcg tttagatcag ctatgtgggg taaacaagga
     7381 ccggtgctac tagatattcc aagagatttg ctagataaag agacgttatc tgatgaaatg
     7441 ttgaatcctg atcaatacag aacagttaac ccacgggtgt ccggtgattt agatgctgtg
     7501 caacaggccg tcgggctact gctctcagca aaaagaccgt tattacttgc tggtggaggt
     7561 gtagtagatt ctgaagcctc taaccaagcg gtacagttgg ctgatttact agacatggcg
     7621 atggttccat cttacggtca tcatgatgca ataccaaaca gccatcccaa ttatattggc
     7681 ccacccggtg gcagagggtc cggagaagct catgaggcga tggatagggc cgatgtgata
     7741 ttggctttag gaaccaaaat taatcaggcg tctagttcat gggactacag tgttataaat
     7801 caagagacaa aaatcattca ggtagatatt gaccccttag aaatcggaag aaattatccg
     7861 gtctcagtgg ggattgtagg tgatgcacag agggtggccc aacagctaat cgcatctata
     7921 tctgtaatag ctgcggatgg taaaactaat gatagttgga agtcggaatt tcaaagtatt
     7981 gctaataaaa gacttcagag attggaatca gaaaaatcgt tgaatgcaaa t
//
LOCUS       DASCNS010000273         2471 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002011899, whole genome shotgun sequence.
ACCESSION   DASCNS010000273 DASCNS010000000
VERSION     DASCNS010000273.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002011899"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="QF838_09680"
     CDS             <1..1110
                     /locus_tag="QF838_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HJO60988.1"
                     /translation="FLGDLNAQIGSVSTAERRILELVDHYDVGALSAIIDGILSATER
                     QVRQFISDWPDGVFKGESHVDDDGFDSKMIPIRAEVTIKGDTMEIDLSGSSPQVTGFI
                     NSAYANTRSIAHAAIMYLAPYDVAKNEGSMGPLTVIAPRGLIVNANPPAPVCMSTNHC
                     AEEIIEAVFKALANAVPAAVNAGFSRRLRYAITGTDPRTGRYFIWHFFMARGGGGASS
                     GNDGWTCVGEVNVAGGIRSPSVEVTEERFPLFVVNHELRPDSAGEGQWRGGLGAECEI
                     IYEGEGEALMNTAGDGVVVPPFGLFGGSPGLPHRYSLVSKGEERVLGSKETGIPVSPG
                     DHIIALSSGGGGYGDPKKRDENSLIWDDKNGYVSS"
     gene            1706..>2471
                     /locus_tag="QF838_09685"
     CDS             1706..>2471
                     /locus_tag="QF838_09685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO60989.1"
                     /translation="MGLADFLVRSAYQMGKTPLLPIFAASLGASDSFAGLIISVSTLT
                     GMIFKPVIGFLSDTWGRKSWLLVGTAFFTFMPFVYSFVDSTQTLFAVRLIHGFATAVY
                     GPVSLAYVAERAGEKKGELLGWFGIAKTSGYVIGPLSAGLLLMYLNPKEVFTIIGILS
                     SAAFLPVLLLPENKLPAQNPKVFSLPKKFLSTIRSGAASSVVWISGSLEGWVYMAVYA
                     VKAFLPIYGIAEGFNVLQVGIFFAVQELVHVMMNPLG"
BASE COUNT          654 a          498 c          609 g          710 t
ORIGIN      
        1 tttctcggtg atttaaatgc acaaataggc tccgtctcaa ctgcagaacg ccgtattttg
       61 gagttggttg accattacga cgtaggagca ttgtcggcaa tcattgatgg aatattgtcc
      121 gccactgagc gacaggttag gcagtttata tctgactggc cagatggagt atttaaaggt
      181 gaatcccacg ttgatgacga tgggtttgat tccaagatga ttccaattcg agctgaggtc
      241 acaataaagg gtgacacgat ggaaatagat ctcagtggct cctcacctca ggtgaccggc
      301 tttataaata gtgcttatgc gaacactcgt tcaattgccc atgcggcaat aatgtatctg
      361 gcaccttacg atgtagccaa gaacgaaggg tctatgggcc ccttgacagt tattgctcct
      421 aggggattga tagtgaacgc aaatcctccc gctccagttt gtatgagtac taaccattgc
      481 gccgaggaga taattgaggc ggtgtttaaa gcattggcaa atgctgtacc agcagccgta
      541 aacgcggggt tttcaagaag gttacggtac gcgatcactg gtacggaccc tcgtactggg
      601 cgatatttca tatggcattt ttttatggct cgtggtgggg gtggtgcctc ttcaggaaat
      661 gatggatgga catgtgtggg tgaagtaaat gttgccggag gtattcgatc ccccagcgta
      721 gaggttacgg aggagcgctt tccactattc gtagtgaatc atgaattacg accagactcg
      781 gcaggcgaag gtcagtggcg aggagggcta ggggctgagt gtgaaatcat atatgaaggt
      841 gaaggagagg ccctcatgaa caccgcaggt gatggcgttg tagtaccacc attcggattg
      901 tttggaggat ctcctggtct accacatcgc tatagtctgg tatccaaagg ggaggaacgt
      961 gtactgggat ccaaagaaac tgggattcct gtttcaccag gcgaccacat tattgcttta
     1021 tcttcaggtg gaggaggata tggtgaccca aagaagcgag atgagaattc tttgatttgg
     1081 gatgacaaaa atggatatgt ttctagttga taggaatata tattcctaag ctttcaggaa
     1141 cctacccact gaaaggcaaa tgagcattct tcaggaaccc taaccctagt aagttttaca
     1201 gagaaatact ctgagcccct ccataccaat ataaaggtgc tgccctaccc caaccggttc
     1261 acttctgccg acactttgcc gaccaaattc ccgacacggg ctgatatcag gcagaaaatg
     1321 tggaacctga atccatattg gtaaggaggc aaagagagag acctcgcctg tggcagaatc
     1381 aaatggaggt tacagtgaaa ggaggacact ttattccgga aaaatcttta catgatattg
     1441 gacagcatat aggggaattt ctttaaaaga cagcttgaag ttttaaagaa actctccgat
     1501 ttcaataccg gttcaacaag agcccttatt ccatggtatt tttggacttt ctttgaaaga
     1561 tttgtgtttc accctcctct tttatgagta atttaagtcc gatagaattg tacggatata
     1621 catttccacg tcacaacaaa gtgattgcag caaaaatata aatataaaac cgaggtatac
     1681 atttgtgagg aagccgatcc ttttcatggg cttagctgac ttcttagtca gatctgctta
     1741 tcaaatggga aaaacaccgc ttctacctat cttcgcagcc tcgctcggag caagtgactc
     1801 gttcgcgggg ctgataattt ctgtttctac cctgacaggc atgattttca agcctgtaat
     1861 agggtttcta tctgatacgt ggggacgaaa atcctggttg cttgttggta ctgccttttt
     1921 taccttcatg ccatttgttt attcattcgt cgattctact caaactctct tcgcggttag
     1981 gctcattcat ggttttgcga cagctgtata tgggcctgtc tctctagcat atgttgctga
     2041 acgtgcagga gaaaaaaaag gtgagttgct cgggtggttt ggaatagcca aaacgtccgg
     2101 atatgtaatt ggcccattat ctgccggttt gctattaatg tatttgaatc ctaaagaggt
     2161 gtttacaata attggaattt taagctcggc agcatttctc ccggttttgc ttttgcctga
     2221 gaataaactt ccggcacaaa accctaaggt tttctcgcta cctaagaaat ttctatcgac
     2281 catccgatct ggggctgcca gctcagttgt ttggatatca ggtagtttgg aaggatgggt
     2341 gtatatggcc gtctacgctg ttaaggcatt tttaccaatt tatggcattg cagaaggttt
     2401 taatgttcta caagtgggga tattttttgc cgtgcaagaa ctagttcatg taatgatgaa
     2461 ccctttagga g
//
LOCUS       DASCNS010000274        10308 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002015873, whole genome shotgun sequence.
ACCESSION   DASCNS010000274 DASCNS010000000
VERSION     DASCNS010000274.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002015873"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="QF838_09690"
     CDS             complement(<1..540)
                     /locus_tag="QF838_09690"
                     /inference="COORDINATES: protein motif:HMM:NF040248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60990.1"
                     /translation="MSRFLVRRLFSMFISIIGATIFIFALTRVGPDPRYLYIPETGGI
                     GIQEDLWEELGEQLGINDPLPVQYGKWVWRMLQGDMGESIGTLRPVSSIIWQHLPATL
                     WLALGAWIFAAVVGLTMGILASVKRATIWDYLARAFALVGQATPQFFTGVLAIMIFSV
                     YMGWFPAGTRGNHDGFFLAW"
     gene            complement(693..2648)
                     /locus_tag="QF838_09695"
     CDS             complement(693..2648)
                     /locus_tag="QF838_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60991.1"
                     /translation="MKIMSKWGISVVTLIALMSLVSVACGGSDDEAEPAAAPAAAPAA
                     ASVAAPAEAGKATVTFAIAEVSEQFGDYPVQPYGASPGELNMGYYDQLLVHDGTDPLS
                     PFLATEWSINDAGNELTLKIREGVNFNTPAEFSSHGDFGELTAHDVAWNLNRQNAMVN
                     PDIRSGLGAQLGGTFGEAVATDDYTVKVPMITEIFWGIPISEFDINDTTVVIDSKKAF
                     DTVGAEAKKLVPVGTGPFTIGEWEPNNRGTVHAIPTHWRETSHIGTFVVVQVPEITSR
                     LAMMQTGQADLGEIDFARLPELPGMGLKFITTMSDKDTMTLSVIWPGNLWTDVNAKTG
                     DALTPWESEVFAVDHPWIGCPWEDKCPYDDTDNPDGISDMEQARLVRWALGMAIDREG
                     INEVLQGGLGTPIYQELMGPKFPGWDPSRTVTAAAIKGYHDKYGGTDWVEYDVPAAEP
                     DYKWPWKIPTDLTEAGRLLDLAGYPKGSDGVRFEIKMNKYRCETGDVCLEQADAVAAG
                     WEELGVKTTLLTEEYGAVVVPRMANRTQAYPVVKNCSVETANYPLDWPPPPSDSTFSR
                     PSWGCSFESKYLDYMYVNINGERDKAKREALHLDMVDYYFYWQLYGGMVQPPRGVAAN
                     PDTIDSWSSRSTAAGAWGRPQHILPAK"
     gene            2883..3335
                     /locus_tag="QF838_09700"
     CDS             2883..3335
                     /locus_tag="QF838_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60992.1"
                     /translation="MNIKIPILVSSFTARFFLGLIFSSFAGFISWVFFFDGSGIDQNV
                     YYVKQSLVIGIPVGFTVSLMWWNTESSGIMMAAQAVVIILFAISLPLLVVNFSNIDVG
                     TTLLGPSLRVPVVSLGDIFKKMLMGAVLGGNVLASLFFLYRSLFHKEI"
     gene            complement(3380..3760)
                     /locus_tag="QF838_09705"
     CDS             complement(3380..3760)
                     /locus_tag="QF838_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60993.1"
                     /translation="MLKWVIVLVLGVVVILVGSAGRIPFTDKSLALSKETRLKAAEEH
                     TPSLSRSEALSRVKTYLVEDCVNGSDYLLNKHRFDATWMRLPRTDDHHVRGMNEWTIN
                     DQSSGAMWRFYEDTGEIITVLGDC"
     gene            complement(3898..4878)
                     /locus_tag="QF838_09710"
     CDS             complement(3898..4878)
                     /locus_tag="QF838_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60994.1"
                     /translation="MTTSTNKITLAELEKSTHDSGISRALYVFRRWPLVPFFILGILI
                     LCALFSPLLAPYEPEKDQLRAVLAMPAWAEACEEGQISNKFKPCNTNGIGLGKDEKKY
                     YFVLGADQLGRDLLSRIIYGARLSLSLAAVAIIIGTIFGTTVGLVAGYYGGIIDEIIM
                     RIVDVFNAIPYLLLAIALVFVLGQSFAVVAFVLALGAWAGIARLVRGQTLQVRNFDYI
                     ALARVAGASTPRIMVRHLLPAVGNTVVVATTLQVGNTILSESILSFLGAGVPPPTPSW
                     GADISNGREYLGSAWWVAFFPGIAIFLTVLSFNFVGDWLRDRWDPRLRQI"
     gene            complement(4888..5835)
                     /locus_tag="QF838_09715"
     CDS             complement(4888..5835)
                     /locus_tag="QF838_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJO60995.1"
                     /translation="MRVFIIRRFLFLIMAILAATLIVFALSRLQGDPRNVMLNVGYVS
                     PEQWEAWGEDFHLDKPLVVQYFIWVGKGFLQGDFGDSLKTGKPVLEMVRGFAPASLQL
                     GLAATLFVLITGIPIGVLSAVKRGTIIDAVGRTFAVFGQALPPFWLGIMLILIFSVNL
                     DWLPSGTRGHDSTGFFDKLTYYIMPAVTLGWLASAGLMRLVRSSMLDVLDSEYIKLAR
                     AKGVDNRSVIWKHAFQNALIPPLTFSALILVGFIGGTVVTETVFAWPGLGQMTYTAIL
                     NNDFPLMTGAVLVFTIVYVFAVFLIDILYALIDPRIRYT"
     gene            complement(5956..8130)
                     /locus_tag="QF838_09720"
     CDS             complement(5956..8130)
                     /locus_tag="QF838_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJO60996.1"
                     /translation="MFKWNKIFGTKMLIGLSAAALLAAGIACGSADDEEAAPAAAPAA
                     APAAAPAAAPAAAPAAAAPAAAAPAASKAPVEQVSTTITIAFDNVGTPQFRNVKGTWP
                     DVLFHGFFGFQEPLLGWEPTFDDQGNAIRDTGEACHAPYVADSWEWILPAKDGSGDIT
                     MDGKLQDKMDLVDLSDPEKQGKIRVHVHEGIDFYRIVDGDMVKIGPLTAEDVAWSFND
                     AGAEDVSSAHSNSSQAYEYYQPWSAVDTYIAEAPNRAFVGDGLQDASSICQDAIWMQS
                     KTLQDETGDTFGIPHGSGPFVVSEWLPAERIEAISRVDHWKQSAAFDKIVFLQANEAQ
                     QRSAMLQTGAADIAMASIQDVGRLEKDGFAFNEGLDAINGNFYYFAGNLWSFAYPPNS
                     KLGEDDLSGKPVMRQGFLPSDKYPWIGDPRLECVGIDRSDPAIQDTPGAGCEVANFEY
                     TFDGSDSKFTADSVGFDYTDTDRFSYETPSMLKAKAFRKALAYSIDRELIAASVTGGY
                     GGAVYGGAAPGMDLHQLHPEYNSAWTYAFDPDKAKAFLAESGVQPGFEFEFFCSQGNG
                     TSLEVCEAVVGQWKENLGLDPFIDSTQYSSRRPTMLGRQIHVPWMTRWGPTSKHGRLG
                     SGGGTYGGGQWPLPAGGWNPGLENNNYWQFREETRVQKKGSPENLATRAIQKAWQYDM
                     TLSTGTVEVPVLIGFNPETVESWDLAPYELPNSFETIVPAGR"
     gene            complement(8363..9538)
                     /gene="nhaA"
                     /locus_tag="QF838_09725"
     CDS             complement(8363..9538)
                     /gene="nhaA"
                     /locus_tag="QF838_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HJO60997.1"
                     /translation="MPVKMLREFFLLESAGGILLVITAGVAIFISNSPLEHYYHDTLN
                     APVIIQIGNFSIDKHLHHWINDGLMAIFFLLVSLEIKREVLDGQLSSRAQISLPAIAA
                     LGGLLFPAFIYLTLNWGNSTTMQGWAIPAATDIAFALGVLTLLGSRVPDSLKLTLVSI
                     AIIDDLAAIVIIAIFYTETLSIYPLGAAFLCILLLFALNKRKISELSPYMVVGTLLWV
                     CVLKSGLHATLAGVIVAIFTPHKSNDKTEPAPLYRLEHGLHLWVAFLILPLFALANAG
                     VSLKGLSTGFFTEPLTLGVGVGLLFGKQIGVMLFTYIGVAFKLCSLPAEIRWSQYYGL
                     ALLAGIGFTMSLFIGELAFEDDKLQTLVRLGVISASLGSGILGYFTLRITSPKQAVA"
     gene            complement(9751..9836)
                     /locus_tag="QF838_09730"
     tRNA            complement(9751..9836)
                     /locus_tag="QF838_09730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9801..9803),aa:Leu,seq:caa)
     gene            complement(9915..10115)
                     /locus_tag="QF838_09735"
     CDS             complement(9915..10115)
                     /locus_tag="QF838_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HJO60998.1"
                     /translation="MKIPLFSRSNKEATDPVCHMTVNPDNPNGGSWDYEGDSYYFCAP
                     GCKIAFQKDPESYLSGEKKIEM"
     gene            complement(10127..>10308)
                     /locus_tag="QF838_09740"
     CDS             complement(10127..>10308)
                     /locus_tag="QF838_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO60999.1"
                     /translation="IAAGILYPLFTSQTVPDWLSPLLGDRGFLNPIVAATAMGLSSIT
                     VVLNALRLKTRKLIN"
BASE COUNT         2862 a         2447 c         2245 g         2754 t
ORIGIN      
        1 ccatgcaagg aaaaatccgt catgattgcc tctagtgccg gctgggaacc agcccatata
       61 gacagaaaaa atcataatag ccaatacccc agtaaaaaat tggggtgtag cctgcccaac
      121 gagtgcaaag gccctagcta gataatccca aatggtggct ctttttacag acgcaagtat
      181 tcccattgtg agaccgacaa ccgctgcaaa aatccaggcg cccaaagcaa gccataaggt
      241 ggcaggaaga tgctgccaaa taatcgagct gacaggacgg agggtgccta tggattctcc
      301 catgtcacct tgaagcattc tccataccca tttgccgtac tgaactggta aggggtcatt
      361 gatacctaat tgttccccca gctcctccca taaatcttct tgaataccaa ttccgccggt
      421 ttcaggtata tataagtagc ggggatccgg accaactctg gtgagagcaa atataaaaat
      481 ggtcgcacct ataatggaga tgaacatgga aaatagtcgg cggaccaaaa atcgactcat
      541 taaataaact aaaacccttg cggaaaggtt ttgtcctcca cggtacaggt gcaccgaact
      601 ttgtgtataa gaggcaaact aagaaagggg gttcactgct cgttagttat acatccgcgc
      661 aggggttaag ctgcgcggat gtatgtgcga atctatttgg caggcagtat gtgttgaggt
      721 ctgccccaag cgccagcagc tgttgatcgc gacgaccacg aatctatcgt gtcaggattg
      781 gctgcaacac ctcgaggagg ttgcaccatt ccaccgtaca attgccagta gaaatagtaa
      841 tcaaccatgt ctaggtgtaa cgcctctctt tttgccttgt cgcgttcacc gtttatgttg
      901 acgtacatgt agtcaaggta cttgctctca aaagaacaac cccagctagg tcggctgaac
      961 gtactgtcag aaggtggggg cggccagtct agtgggtagt tggctgtctc cacgctacag
     1021 tttttaacca ccggataagc ctgagtccta ttagccattc ttggaacgac cactgctcca
     1081 tactcttcag ttaacagagt agttttgacc ccgagttctt cccatccagc ggccactgcg
     1141 tcagcctgtt caagacaaac gtcccctgtt tcacagcggt acttattcat cttgatttca
     1201 aaccggacgc catcggaccc tttgggatat ccggctaggt cgagtaagcg tcctgcttcg
     1261 gtcagatctg taggtatctt ccaaggccac ttgtagtctg gctctgcagc aggaacatcg
     1321 tattctaccc aatcagttcc gccgtactta tcatggtatc ccttgatagc ggctgctgtg
     1381 actgtacgac ttggatccca acctgggaac ttaggtccca tcaactcttg gtatattgga
     1441 gtcccaagtc ctccttgaag gacctcgtta attccttctc tgtcaatagc cataccaagg
     1501 gcccatcgaa caaggcgagc ttgttccatg tcgctgattc cgtctgggtt gtcagtatca
     1561 tcatatggac acttatcttc ccaaggacat ccaatccaag gatggtcaac tgcgaacacc
     1621 tcagattccc aaggagttag agcatcaccg gtttttgcgt ttacatctgt ccacaggtta
     1681 cctggccata taacgctcaa ggtcattgtg tccttgtctg acatcgtggt aatgaacttc
     1741 agccccattc caggtagttc aggtagtcgt gcaaagtcta tttcgccgag gtctgcctga
     1801 ccagtctgca tcatagcaag cctgcttgtg atttctggca cctggacgac aacaaatgtt
     1861 ccaatatggg acgtttctct ccagtgggtt gggatagcgt gtactgttcc ccggttattg
     1921 ggttcccact caccaatcgt gaatggtccc gtaccaactg gaactagttt tttcgcttct
     1981 gccccgactg tatcaaaggc ctttttactg tctatgacga ctgtggtgtc gtttatatcg
     2041 aattcactga tcggtattcc ccaaaaaatt tcggtaatca tggggacttt gacagtgtaa
     2101 tcgtctgtgg caacagcctc accaaaagta ccaccaagct gtgcgccaag accagatcgt
     2161 atgtctgggt tcaccattgc attctgcctg ttaaggttcc atgcaacgtc gtgtgcagtt
     2221 aattctccga agtctccatg ggaagagaat tccgcagggg tattaaaatt aaccccttcc
     2281 cttatcttta aggtgagttc gttgcctgca tcatttatgc tccattccgt agcaaggaac
     2341 ggggataaag ggtcagtccc atcgtggact agcaattggt cgtagtagcc catattcagc
     2401 tcacctgggc tagcaccgta aggctggacc ggatagtcac caaactgttc agacacctct
     2461 gcaatcgcaa aagtaacagt tgcttttccg gcttccgctg gggctgccac tgaagctgcc
     2521 gctggagctg ctgctggagc tgctgccggt tccgcttcat catcggagcc accgcaagcc
     2581 actgaaacta gagacataag agctatcagt gtgacaactg agatccccca tttactcata
     2641 atcttcatga gatttacccc cttgattcat tttcttatct gaattcaaac accaccacgc
     2701 acctttaacc gcgactgttc cagtcacaag ttggcccgtc catcagtagt ggaattatca
     2761 gtatttagag cccatcataa ataaagagaa ctcgtattgc aatagtagtt attttcaacc
     2821 agaatttaat tctggagacc gaattgtttc agtaagccct ttttttcgaa taaaatagcg
     2881 agatgaacat aaaaatacca atattagttt catccttcac agctagattt tttcttggtc
     2941 ttattttttc ctcgtttgct ggctttatat cctgggtatt tttctttgac ggtagcggaa
     3001 ttgaccaaaa tgtctattac gtaaagcaaa gtctagttat tggaattcct gtgggtttca
     3061 cggtgtcttt aatgtggtgg aatacagaaa gttctggaat catgatggct gctcaagcag
     3121 ttgttataat tctatttgct atttccctac cgctcttagt agtaaacttt tcaaatattg
     3181 atgttggcac caccttgctt ggcccatccc ttagagtgcc agtggtatct ttgggggaca
     3241 tttttaaaaa aatgttaatg ggagcagttc taggaggaaa cgtcctagct tcactgtttt
     3301 tcctatatag atcacttttc cataaagaga tataaagtac ctattgaaga ttctttgtat
     3361 gaatcttatt tcagctgaat tagcagtcac caaggacagt tataatttcg ccagtatcct
     3421 cataaaaacg ccacattgct cctgatgatt ggtcatttat agtccattcg ttcattccac
     3481 gcacatggtg gtcgtcagta cgcggtaatc tcatccaggt agcatcaaac cgatgtttgt
     3541 ttaataagta atcgctacca tttacgcaat cttcgaccaa ataggttttt accctagaaa
     3601 gcgcctcgga ccgtgaaaga ctgggggtat gttcttctgc cgccttcaac ctcgtttctt
     3661 tagatagagc aaggctttta tcagtgaaag gaattctgcc tgccgaccca accagaatca
     3721 cgacaactcc taacactaga actatgaccc actttaacaa ttcttctctt ccccttctca
     3781 gtcagtaatt attttcagtt gcacgttaaa ttatagaact atcaacagcc tggtaaattt
     3841 ggacaaacag atttccaggc gagaaagcga gcatttttcc ttgaatgctt aaataggcta
     3901 aatttgccga agtctaggat cccatctatc ccgtagccag tcgccaacga agttaaatga
     3961 tagtacggtg aggaatatcg caatacctgg gaagaaagct acccaccaag cggatccgag
     4021 gtattccctt ccgttggaga tatctgctcc ccatgaaggt gtaggtggcg gtacccctgc
     4081 ccccaaaaag gacaatatac tttcggacag gatagtattt cctacctgta gtgtagtggc
     4141 gacaacaaca gtattcccca ctgctggaag taggtgtctt accattatcc ttggcgtgga
     4201 agcccctgcc acacgcgcta gagcaatata atcaaaattc ctcacttgca aagtttgacc
     4261 tctaacaagc ctcgctattc cagcccaagc cccaagagcc aacacaaaag cgactaccgc
     4321 aaaactctgc cctagtacaa aaaccaacgc gatggcaagc aataagtacg ggatagcatt
     4381 gaagacgtca acgatacgca taatgatttc gtcaatgatg ccaccatagt atccggctac
     4441 caaacccaca gtggtaccaa atattgttcc tataattata gctacagcag caaggcttaa
     4501 cgaaagacgg gctccgtaaa tgattcgact taataaatca cgacctaact ggtctgcccc
     4561 gaggacaaaa tagtattttt tttcatcttt cccaagtcca ataccatttg tattacatgg
     4621 cttgaatttg ttagatattt gaccttcctc acaggcttca gcccatgcag gcatagccaa
     4681 aacagctctc aattggtctt tctctggttc atagggtgct aacaatggag aaaatagggc
     4741 gcaaagaata agaatcccta agatgaagaa aggtacaaga ggccaacgac ggaacacata
     4801 tagtgcccta gaaatacctg agtcatgcgt agatttctct aattcggcca gagttatctt
     4861 atttgttgat gtagtcaaaa gtaatcctta cgtgtatcta attctgggat cgattaacgc
     4921 atacagaatg tcgattaaga atacggcgaa aacatagaca atagtgaaaa ccaatacagc
     4981 ccctgtcatc aggggaaagt cgttattgag tatggccgtg taagtcattt gacctaaccc
     5041 tggccatgca aaaacagtct cagtcaccac tgtgccacca ataaacccga ccagaatcag
     5101 tgcagaaaag gtcagcggtg gaattagggc attttggaaa gcatgtttcc atatgacgct
     5161 gcgattatca acgccttttg ctcttgccag cttaatatat tcagagtcga gaacatcaag
     5221 catcgaagat cttacaagtc gcattaaccc ggcagaggcg agccacccga gggttactgc
     5281 gggcattatg taataggtga gcttatcaaa aaagcctgtc gaatcgtgcc cccgagtacc
     5341 cgagggcagc caatccaggt taactgagaa aatcagaatg agcataatcc caagccaaaa
     5401 aggaggtaaa gcttgcccaa acactgcaaa tgttcttccg acagcgtcaa tgattgtgcc
     5461 tcttttgacg gctgacagca cccctatcgg aattcccgtt ataaggacga aaagtgtggc
     5521 agcaagccca agttgcaaac ttgccggtgc aaaacctctc accatttcta gcacaggctt
     5581 acctgtttta agagagtcac caaaatctcc ctggagaaat cctttaccga cccatatgaa
     5641 gtactgaaca actagtggtt tgtctaagtg aaaatcttca ccccatgcct cccattgttc
     5701 tggtgacaca taaccgacgt ttagcatcac gttgcgagga tcgccttgta ggcgagagag
     5761 tgcaaacacg atcaaagtgg ctgccaaaat agccataatg aggaacaaaa accgtcttat
     5821 tatgaatact ctcattagat atacctaagc gcagtgattc gccaattgcc cggcgaacgt
     5881 aagatgaagg ttcactgacg caccttcccc aacgggaata agtgcgtcag tttacttgct
     5941 taaatttgct tttacttacc ttccggcagg gactattgtc tcaaacgagt taggaagttc
     6001 atacggtgct agatcccaac tttctactgt ctccggattg aatccgatca aaaccggaac
     6061 ttctacggtg ccggtcgaca atgtcatgtc gtactgccag gctttttgta ttgcccgagt
     6121 tgctaggttc tcaggagagc cttttttctg cactcttgtc tcttcacgga actgccagta
     6181 attattattt tcaagacctg gattccatcc accagctgga agaggccatt gaccgccacc
     6241 ataggtacca ccacctgatc ctagcctacc gtgtttgctg gtaggacccc atcgggtcat
     6301 ccaaggaaca tgaatctgtc ggcccaacat tgtaggacgt cgggatgagt actgtgtgct
     6361 gtctatgaaa ggatcaagcc caagattctc tttccactgt ccgacgacgg cttcacatac
     6421 ttcaagagaa gtaccgttac cctgtgagca gaagaattca aattcgaacc ctggctgcac
     6481 accagactct gctaagaaag cttttgcttt atcggggtca aacgcataag tccaagccga
     6541 gttgtattca gggtgtagct gatgtaggtc cattcctggg gcggcgccac cgtataccgc
     6601 tccaccatat ccacctgtaa ccgatgcagc aattagctca cggtcaatag aatacgctaa
     6661 tgcctttcgg aaggctttgg ccttcaacat tgagggggtt tcataggaaa acctgtcagt
     6721 gtctgtatag tcaaatccca ctgagtcagc ggtaaattta gagtcggaac catcaaatgt
     6781 gtactcaaaa ttggctactt cacaaccagc accaggggtg tcctgtatgg ctggatctga
     6841 tcggtcgatt cccacgcatt ccaatctagg gtcaccaatc caagggtatt tatccgaagg
     6901 cagaaatcct tgtctcatta ctggtttacc cgacaagtcg tcttcgccaa gtttggagtt
     6961 aggtggatat gcaaatgacc agaggttacc tgcgaagtaa tagaaattac cattaatagc
     7021 atctaggccc tcgttaaacg cgaacccatc tttttcaagg cggcctacgt cctggattga
     7081 tgccatggct atgtcagcag ctccagtttg gagcatagca gagcgctgtt gagcttcatt
     7141 cgcctgcaaa aagactattt tgtcaaatgc agcgctttgt ttccagtgat ctaccctaga
     7201 aatagcttca attctttcag caggtagcca ttcgcttact acgaaaggac ctgatccgtg
     7261 cggaataccg aatgtgtctc ctgtttcatc ttggagcgtc ttggattgca tccatatcgc
     7321 atcctgacaa attgaggaag catcctgtag tccatcacca acgaaagccc tgttaggtgc
     7381 ttccgcaatg tacgtatcta cagcagacca aggttggtaa tactcgtaag cctgagagga
     7441 gttagagtga gcggagctaa cgtcttcagc accagcgtcg ttgaaggacc atgcaacgtc
     7501 ttcggcagtc aatggtccaa ttttgaccat gtcgccgtcc acaattctgt aaaaatcaat
     7561 gccctcatgg acgtgaactc taattttccc ttgcttctct ggatcactta agtcaaccag
     7621 atccattttg tcttgtaatt taccatccat ggttatgtca ccactgccat ctttagcagg
     7681 aagtatccat tcccaggaat cagctacgta tggggcgtga caagcttcgc ctgtatcccg
     7741 gattgcgttg ccttggtcgt caaaagttgg ttcccatccg agcaatggtt cttggaaacc
     7801 gaagaacccg tggaatagaa cgtcaggcca tgtgccttta acattcctaa attgcggtgt
     7861 cccaacgttg tcaaacgcga ttgtaatagt ggtgctcact tgttccactg gtgcctttga
     7921 ggcagcaggt gcggcagcgg caggtgcggc agcagcaggt gcagcagcag gtgcagcagc
     7981 aggtgcagca gcaggtgcag cagcaggtgc agcagcaggt gcagcttcct catcgtctgc
     8041 agagccacag gcaataccag cagctagtaa agcagctgca gacaggccta ttagcatctt
     8101 ggttccgaaa atcttattcc acttaaacat aactgatcct ccaacaaact gtgtaatgct
     8161 ttaggttgaa ttatcttaat ttataagggc caatacggca ttatctcttt ctgtgagtat
     8221 ttattaactc acagatcaac actatcccac agttagatat cactgtaaag gacggaactc
     8281 ttcactccag aaggaaatcg tgaggatttt gaaccaacga tcagccttcg aatcctaagt
     8341 ataagcgcca aaaatattga gtctatgcca ccgcttgttt ggggctagtg attctcagag
     8401 tgaaataccc gagtatgcca gaacctagag aagcgcttat aactcccaac cggactaaag
     8461 tttgtaattt atcatcttcg aaagccagtt ctccaatgaa caaactcata gtaaatccta
     8521 taccagcgag aagcgctaac ccataatatt gactccatcg aatctcggca ggaagcgagc
     8581 agagtttgaa agcaactcct atgtaggtga atagcatgac tccaatttgc tttccaaaaa
     8641 gcaatcccac tcctacacct agggtaagtg gttcagtgaa aaagccagta gatagacctt
     8701 ttaaagagac gcctgcgttg gctaacgcaa ataaaggcaa tatcaaaaat gcaacccaga
     8761 gatgaaggcc gtgttctagg cgatatagag gcgcgggttc ggtcttatca tttgatttgt
     8821 gaggagtgaa gatagcaacg ataactcctg ctaaagtggc gtgaagacca gactttaaaa
     8881 cacaaaccca aagcaatgtc ccaacaacca tgtaaggtga cagctcagaa atttttcttt
     8941 tattgagagc gaaaagaagc aaaatacaca ggaaggctgc gcccaatgga taaatcgata
     9001 gggtctcagt gtaaaaaatg gcaataatta ctatcgctgc taaatcatct atgattgcga
     9061 tggataccaa ggttaatttt aaagagtccg gcactctact acccaaaagt gtcaacacgc
     9121 cgagggcaaa ggcgatatca gtggcagctg ggatcgccca accttgcatt gttgtcgaat
     9181 taccccagtt caaagtcaga taaataaaag caggaaataa taaccctccc agtgccgcga
     9241 ttgcaggcaa gcttatttga gccctacttg ataactgtcc gtcgaggact tcccgtttta
     9301 tttccaaact caccaataaa aagaaaatcg ccatcaaccc gtcattgatc cagtggtgga
     9361 gatgtttatc aatggaaaaa tttccgatct gaataattac aggggcgttt agggtgtcat
     9421 gatagtaatg ctctagtgga gagttactaa tgaatatggc gacgcctgca gttatgacta
     9481 aaagtatgcc tccagcagac tctagtaaaa aaaattctct aagcattttt acaggcataa
     9541 tattctcctg gaaaatatta cctctaatag agctagagat gtatatggat tactctcttc
     9601 tatcaagcaa ttaaggcgat ttggtgttat gaaacctaaa atccaacttt ctatcgatag
     9661 atggcggtag tgggcatttt agggaatcta gaaaataccg tcgggttgat aatttacaag
     9721 attaaagctt gttttaggta tgaaaaggtt tggtgccgaa ggcgagagtc gaactcgcac
     9781 gagtgttacc tcaacggttt ttgagaccgc cgcgtctacc gttccgccac ttcggcatgc
     9841 aaaggaatgt aattataaag atggttaagt ttggctgcta gtaggtagcc gttttaaatg
     9901 gtgaattctt gaagctacat ttctattttc ttttcccctg aaaggtaact ttcaggatct
     9961 ttttggaatg ctattttgca tcctggggca caaaaatagt atgagtcccc ttcatagtcc
    10021 caagatccac cgttcggatt gtcaggattc acagtcatat ggcagacggg atcagtagct
    10081 tctttgtttg atcttgaaaa tagagggatt ttcattggaa ttcaccttaa ttaattagtt
    10141 ttctagtttt taagcgaaga gcattcaaaa caacagttat cgagctaagc cccatcgctg
    10201 ttgccgctac tattgggttt aaaaaacccc gatctcccaa aaggggagaa agccaatccg
    10261 gtacggtttg tgaagtgaat aaaggatata agatacctgc tgcaatag
//
LOCUS       DASCNS010000275         8032 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002025967, whole genome shotgun sequence.
ACCESSION   DASCNS010000275 DASCNS010000000
VERSION     DASCNS010000275.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002025967"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="QF838_09745"
     CDS             complement(<1..1876)
                     /locus_tag="QF838_09745"
                     /inference="COORDINATES: protein motif:HMM:NF015644.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HJO61000.1"
                     /translation="MASAPFPSFDFDPSSIKEKLGSRIKWIVLASILIPIFVLSWIAK
                     GIFTDYLWYSNMGFEDVFITILMTKVVLFLIGFVFVLALVSANLFYVNRKTAGPLDAP
                     LSENLIDIVRKLLLVVCVLVSVVLAVILGSVIASKWELFLRFSNAAGFGIKDPLHGND
                     LSLYVFELPIYAFLQGWLLVCVGATIVATTALAFLNFTLRGTAFSLTTPLRNHLLVLG
                     ALAILILSAGYWIDRLELVRSEHGIIYGAAYADVYSRLPAYLVLSIAGILVSILMLVG
                     SFMGKIKLSLAPVGIWVILIIGLGTVWPSVVQQFSVDPDEFVKETKYIERNIEFTKLG
                     FGLEEVEETFYEAEGEITAELIEENIPTVENIRLWDYKPLTNVYKQIQLIRPYYDFKD
                     ADVDRYTINGEYRQVLLAAREVAPEKLKEEAQTWVNQKLFYTHGIGIAMSPATEFTPE
                     GRPIFFAKDIPANGEIPISSSEQGASPEIMVKNPRIYYGENTKDYVIVDTNTQELDYQ
                     TGEGDLIVRSYEGSGGISLGSYFRKLLYAWQMRDINILISNQLNEDSKIQYRRSIEER
                     VSTLAPFLKLDKDPYIVAANDQLFWIQDAYTTSDKFPYSQPTDEDDTSLGHYNYIRNS
                     I"
     gene            complement(1890..2780)
                     /gene="argB"
                     /locus_tag="QF838_09750"
     CDS             complement(1890..2780)
                     /gene="argB"
                     /locus_tag="QF838_09750"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HJO61001.1"
                     /translation="MFSEINHSESDDRLERPQSMKSNDECVDQRPSASDVPINQQSLT
                     VIKIGGSTLGSHDTTLMDLVELQKEGEKFVVIHGGGKTISEWMEKQGVRPKFVNGLRV
                     TDSQSLDIVVAVLTGVINKSLVASINALGGRAIGISGADGNMVSAEIADPELGYVGKI
                     RSVDTAPIEAILEGGYIPVIAPVGIHSGSGHQGKLLNINADTVAGYVSSSINANRMVF
                     LTDVEGVLDSSKRLISRMTKRQADSLVASHVIDGGMIPKMEACIEALLGGAISQIIDG
                     RAPGALKDVISGHNLGTRIG"
     gene            complement(2791..4071)
                     /gene="argJ"
                     /locus_tag="QF838_09755"
     CDS             complement(2791..4071)
                     /gene="argJ"
                     /locus_tag="QF838_09755"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HJO61002.1"
                     /translation="MADIFRHFLSAFLTGRLSMEKGEPRLIEIINGGGVTSATGFTAG
                     STYAGLKTEAGFPDLGIILSDLPANSAATFTTNSIESPSVTVSRKRKKAGISRGIVAN
                     SGCANCSVGPQGLTDAEELASLAASHVGVSGDDLYVASTGMIGVELPMALIRQNIVNI
                     TLSNDGGTEFAKSIMTTDSKHKERAVSFMHDGKTVTVGGAAKGVGMIHPNMATMLCFI
                     TTDADVDQSFLQSVLSESVAVSFNMTDVDGDQSTNDSVIVLANGASGTEQIQGNSAGA
                     DSFVEAIRYVCTELAKELARDGEGAQRLIEVTVEGAHTIEDARKAAREIASSSLVKAM
                     VHGKDPNWGRLVMALGKSSIQLEEQKIDIFISDIHIVHNGIAIPYLKDAVVSAMNSDQ
                     VDFRIDLNVGNSTATGWGCDLTEEYVIFNSAYST"
     gene            4245..6479
                     /locus_tag="QF838_09760"
     CDS             4245..6479
                     /locus_tag="QF838_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009818589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HJO61003.1"
                     /translation="MTITNTNQKFTSVGSRPIRHDGFDKVTGKALYGADIELPGLIHG
                     KILRSPHAHAKILSIDTSEAEKMGDVHAVITNHHFPAQEDKSVSKIPGPAMNLKQQTS
                     NILASDKVLYKGHAVAAVAANSAHAAEEALALIKVEYEQLPHVSTGEEAISDGAPLLH
                     DQYKNNIASHDVLEMGDVDKGFGQVDLVVEREFRTSTVHQGYIEPHSATGWWTANNKI
                     TIWGSSQGHFQIRDRTSLVLGVPVSAIKVVPMEIGGGFGGKTTIYLEPVAALLSKITG
                     YPVKITMSRSEILEATGPTSGSYMKVKIGATKDGKITSAQADLKFEAGAYPGSPVGAA
                     ANCIFTPYAIENVKIDGYDIVNNKPKTNAYRAPGAPIGAFAAETLIDELAEKIGMDPL
                     DLRIMNGAKQGTPRVSGINNPLIGCIETFQTTKEHDHYQSPKSSKKSGRGVASGFWIN
                     GSGAACAVANVNFDGTVNLTIGSMDIGGLRPVAAQHVAEVLGIHVDDVIPQVGDTETI
                     GYTSMTGGSGGAFKTGWASYEAAQDVKRQMLERAAEVWETSLDDIELENGIFKHSADT
                     ELKMSFQELAGHLPETGGPVVGRANLDPRGPGSAFATHVVDLEVDIETGKVTILRYTA
                     AQDAGKAIHPSYVEGQIQGGAVQGIGWALNEEYFINDSGGMTNASLLDYRMPTSLDLP
                     MIEALIIEVPNPLHPYGVRGVGEVAIVPPLAAIANAIYDAIGVRMTELPMNPAAVRKA
                     INGE"
     gene            6484..7233
                     /locus_tag="QF838_09765"
     CDS             6484..7233
                     /locus_tag="QF838_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013530.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61004.1"
                     /translation="MKRILVTGMSGLIGGLLKDHLISIGEYQLSALNRSFVEGISTHS
                     ADIAELEQIRPAFENIDVVVHLAAYLGSDSFEEQLPTNIIGTYNVFEAARAAGVKRVV
                     YASSGSTVRGVENIYPYGEIAQGNYENVKGPIPLITHQDIRPGGVYGATKVWGEALAR
                     HFSDTYDMSILCVRIGSVTKENIPSDARQKSIYLSHKDVCQILQLSIDAPSELKYDVF
                     LATSNNRWSYRDLEHPRKILGYEPLNSAEDL"
     gene            7234..7902
                     /gene="coaE"
                     /locus_tag="QF838_09770"
     CDS             7234..7902
                     /gene="coaE"
                     /locus_tag="QF838_09770"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HJO61005.1"
                     /translation="MPLVIGITGAIASGKSTACQILENLGGTHCDADKLVHRMYDPGK
                     PAFHRIVESFGDEVVGTDGFIDRKQLGSKVFGNPEKMSELTTAIGDIKAEVSGVMKQW
                     RNTLSLHEVGLMEAVNFIEAGYGIYSDITWLFAVDDEMAIKRLKERNSFSEEEATQRL
                     SSQKKWQDRAPASDIVTMNNGSLKELKESVTKQYAELTDNYSKGLLPPSKYINWWEKH
                     SPKD"
BASE COUNT         2463 a         1775 c         1676 g         2118 t
ORIGIN      
        1 tgatactatt ccttatgtag ttgtagtgac ctaagctagt atcgtcttca tccgttggct
       61 gcgagtaagg aaatttatcg gatgtagtat aggcatcttg gatccagaac aactgatcat
      121 tagccgccac tatataagga tctttatcca gttttagaaa tggcgccaga gtagagactc
      181 tttcttcaat tgaacgccgg tattggatct tactatcttc atttaattga ttactgatca
      241 ggatatttat atccctcatt tgccaggcat agagtagctt cctaaagtaa gaaccgagcg
      301 agatccctcc cgacccttca taacttctta ctattaagtc tccttctccg gtctggtaat
      361 ccagttcttg cgtattcgta tcaacaatca cgtagtcttt tgtgttttca ccgtaataaa
      421 ttcttggatt cttgaccatt atttcagggg aagcaccttg ctctgaagat gaaataggga
      481 tttcaccatt agctggaata tctttagcaa aaaaaatggg cctgccttcc ggggtaaatt
      541 ctgtggctgg gctcatggca ataccaatac catgggtgta aaacaatttt tggttgaccc
      601 aggtttgagc ttcttctttt aatttttctg gggcaacttc tcttgctgcc aacagtactt
      661 gtcggtattc accatttatg gtgtacctat caacgtcagc gtctttaaaa tcgtaataag
      721 gtcgaatcaa ttgaatttgt ttgtaaacat ttgtcaatgg tttgtaatcc cagagtcgta
      781 tgttttcaac ggtaggaata ttttcttcta ttaactcggc agttatctct ccttctgcct
      841 catagaaagt ctcctctacc tcttccagac caaacccaag cttagtgaat tctatattgc
      901 gctcgatgta ttttgtttcc ttaacaaact cgtcagggtc aactgaaaat tgctgaacaa
      961 cagagggcca gactgttcca agcccaatta tcaagatcac ccaaatacct acaggagcca
     1021 aggataattt aattttaccc ataaaggatc ccaccagcat aagtatagaa acgagaatac
     1081 cagctatgct taaaaccaaa taggcaggta gccgtgagta aacatctgcg tatgccgccc
     1141 cataaataat accgtgctcg gatctaacga gttccaacct gtcaatccaa tatccagcgg
     1201 aaaggatcaa aatagctagt gcacctaaaa caagtaggtg attacgaagt ggtgtggtta
     1261 aggaaaaagc ggtgccccta agggtaaaat tcaaaaaagc caatgcagtc gttgccacaa
     1321 tagtggctcc tacacaaact agaagccaac cttgtagaaa ggcataaatg ggtaattcaa
     1381 aaacatataa agaaagatca ttaccgtgta agggatcttt aatcccaaat ccagccgcgt
     1441 tgctaaatcg gaggaaaagt tcccatttgg aggcaatcac ggatcctagt attactgcta
     1501 atacaacaga taccaacaca catactacaa gcaacaattt cctaacaata tcaattaaat
     1561 tctctgaaag aggcgcgtct aatggtcctg ctgtttttct gttcacatag aaaagatttg
     1621 ctgaaactaa tgctaagaca aacacaaaac ctatgagaaa taatacgact ttggtcatta
     1681 gaattgtaat gaaaacatct tcaaagccca tattcgaata ccaaaggtaa tctgtgaaaa
     1741 tccccttagc aatccatgac agaacaaaaa tagggataag aatagatgct aacactatcc
     1801 attttatccg gcttccaagt ttttctttaa ttgaagacgg atcgaaatca aatgagggga
     1861 agggggcact tgccatgtta taacctttat tagcctattc gagtacctaa attgtgaccg
     1921 gaaattacat cttttaaagc ccctggggcg cggccatcaa tgatttgcga aattgctcca
     1981 cctaaaagag cttcaataca ggcttccatt ttaggaatca ttccaccatc aatcacatga
     2041 gatgcaacca aggaatcagc ctgacgtttt gtcatacgag aaatcaaacg tttggaagag
     2101 tctaaaactc cttccacatc ggttagaaat accatccgat ttgcattaat agaagaagac
     2161 acataccccg ccacagtatc agcatttata ttcaataatt tgccctggtg accggaacct
     2221 gagtgaatcc ctacaggagc tattaccgga atatagccac cttccaatat tgcctctata
     2281 ggagccgtat caacagacct aatcttaccg acgtaaccta actcaggatc agcaatttct
     2341 gcgcttacca tattaccatc ggcgccggaa ataccaatcg ctcttcctcc caatgcgttt
     2401 atagaggcga caagagattt atttataact ccagtaagaa ctgcaacaac aatatcaagg
     2461 ctctgtgagt ctgtgaccct caatccattg acaaatttag gcctaacacc ttgcttttcc
     2521 atccactccg atattgtctt acctcccccg tgaattacaa cgaatttctc cccctctttt
     2581 tgtaattcca ctaaatccat cagggtagtg tcatgactac ccagagtact tccaccaatc
     2641 ttgatgacag tcagtgattg ttgattaatc gggacatcac tggcacttgg tcgttgatcc
     2701 acacactcat cgttgctttt catgctttgt ggtctttcca aacggtcatc ggattcagag
     2761 tgatttattt cgctaaacat ttttaaacca tcaggtgcta tatgcagaat taaaaattac
     2821 atattcttca gtaagatcac atccccaacc ggttgctgtc gaatttccta cattgagatc
     2881 tatcctgaaa tccacttggt cagaattcat tgcacttaca accgcatctt tcagataggg
     2941 tattgcgatg ccgttatgaa caatatggat atcacttatg aaaatatcta ttttttgttc
     3001 ctcgagctga attgagcttt tccctagagc cattaccaat cgtccccagt tgggatcttt
     3061 cccgtgtacc atcgccttca ccagagaaga ggatgcaatt tctctagcgg ctttgcgcgc
     3121 atcttctatt gtatgggccc cttcaacagt gacctctatg agcctttgtg ctccttctcc
     3181 atcccttgcc aactctttgg caagctccgt acagacatac ctaatggctt caacaaaact
     3241 atcagcacct gccgaatttc cctggatctg ttcagttcct gaagcaccat tcgccaaaac
     3301 aattacggaa tcgttagtgc tttgatcacc atcaacatca gtcatattaa aagaaacagc
     3361 tacggattcg gaaaggacac tttgtaaaaa actttgatca acgtcagcat ctgtagtaat
     3421 gaaacatagc attgtcgcca tattcggatg aatcatccct acccctttcg ccgctcctcc
     3481 aactgtgaca gtctttccgt catgcataaa tgaaaccgcc cgctctttat gcttggagtc
     3541 tgtagtcatt atagatttcg caaactctgt accaccatca ttggaaagag ttatattgac
     3601 aatattctga cggatcaatg ccatcggaag ttccactcca atcattccgg tgcttgccac
     3661 ataaagatca tctccagaca caccaacatg actggccgcc aaggaggcaa gttcttctgc
     3721 gtcggtcagc ccttgggggc ctacactgca attggcgcat ccgctattag caacgatacc
     3781 ccttgaaata ccagcctttt ttctcttacg actgactgtc actgagggtg attcgatgct
     3841 gttagtggta aatgtcgccg ccgagttggc cggtaggtca gataaaatta ttcctaagtc
     3901 aggaaatccc gcttcggttt ttaaccctgc atatgtggat ccagctgtga agccagtagc
     3961 tgaggtgacc ccacctccat ttattatttc tatcaacctg ggctctcctt tttccattga
     4021 caacctacca gttagaaaag cagacaaaaa atggcggaat atgtccgcca ttcaaaagct
     4081 tcgaaattat agcatatagg tacaccttag gtacgaaaaa atggtattcc ctatgctaca
     4141 atccagcgga aaggtacatc attcaaaaga ctgttcccaa acttaatgta gtaaacaaaa
     4201 ggagcatcat tcaaacattt ttggattagt aaggacataa attaatgaca atcacaaaca
     4261 caaatcaaaa atttacctca gttggcagcc gcccaataag acacgatggt tttgacaaag
     4321 taacgggaaa agctctctac ggggccgata tcgagctgcc tgggcttatc cacgggaaaa
     4381 tattgcggag tcctcacgct cacgccaaga tactatcaat agacacctca gaagcagaga
     4441 aaatgggaga tgttcatgca gtaatcacaa accaccattt cccagcccaa gaagacaagt
     4501 ctgtttcaaa aatccccggt cctgcaatga atctaaaaca acaaactagt aatattttag
     4561 cctcagacaa ggtcttatat aagggacatg ctgtggcagc tgtagcagca aacagtgctc
     4621 atgcagcaga agaggccctt gccctcatta aagtggaata tgagcaatta ccccatgtgt
     4681 ctaccgggga agaagcaata tcggatgggg ccccgttatt gcacgatcaa tacaaaaaca
     4741 acattgccag tcacgacgtt ttagaaatgg gagatgtgga taagggtttc ggtcaagtcg
     4801 atcttgtggt tgagagagaa ttcagaacct caactgtgca ccaaggctat atagaacccc
     4861 attcagcaac tggctggtgg actgcaaaca ataaaataac catttggggc agttctcaag
     4921 gacatttcca aattagagac agaacatctt tggtcctagg ggtgcctgta tcagcaatca
     4981 aagtggtacc aatggaaatt ggaggaggat tcgggggtaa aaccacgata tatttggagc
     5041 cagtggcggc tttgttatct aaaattacag gatatccagt aaaaattacg atgtcacgtt
     5101 cggaaatctt agaagctacg ggtccaacct ctggttcata catgaaggta aagattggcg
     5161 ccactaaaga tgggaaaatc acctcagctc aagcagattt gaaatttgaa gctggtgctt
     5221 atcccgggtc ccctgtagga gcagcagcaa attgcatatt cactccatac gcaatcgaaa
     5281 acgtaaaaat agacggctat gacatagtaa ataataagcc aaagaccaac gcgtaccggg
     5341 cacctggagc acctatcggc gcgtttgccg ctgagacttt aatcgatgaa ttggcggaaa
     5401 aaattggtat ggacccgttg gatctaagga taatgaatgg tgccaagcag ggtacaccta
     5461 gagtttccgg aataaataat cctctaattg gatgcataga aaccttccaa accaccaaag
     5521 aacacgacca ttatcaatcc ccgaaaagca gcaaaaaatc aggacgagga gtagcctccg
     5581 ggttttggat caatgggagt ggagcagcct gcgctgtggc taacgtaaat ttcgatggca
     5641 ctgtaaacct tactataggt tctatggata tcggaggttt aagaccagtt gcggcccagc
     5701 atgttgcaga agtactagga atacatgttg atgacgtaat tccacaggtg ggagataccg
     5761 aaactatcgg atatacctcc atgacaggag gcagtggtgg agccttcaaa acgggctggg
     5821 ccagctatga ggctgcccag gatgtcaaaa gacaaatgct agaaagagcc gcggaggtat
     5881 gggaaacatc cctcgacgat atagaacttg aaaacggtat cttcaaacat tctgctgaca
     5941 ccgagttgaa aatgtcattc caggaattgg caggtcatct tccagaaaca ggaggaccgg
     6001 ttgtgggtag agctaacctt gatccaagag gtccaggtag cgcgtttgcc actcatgtgg
     6061 ttgatctcga ggttgatata gaaacaggaa aagtgaccat tctcagatac acggctgctc
     6121 aggatgccgg caaggcgata caccctagtt atgtagaagg ccaaatacaa ggaggtgccg
     6181 tgcaagggat tggatgggcg ttaaatgagg aatatttcat aaatgattca ggaggtatga
     6241 ccaacgctag cctgctcgac tacaggatgc ccacttcttt ggatttgccg atgattgaag
     6301 ccctgataat agaagtgccg aatccgctgc atccatatgg agtccgtgga gttggggagg
     6361 ttgcaatagt acctccgctg gcggcaatag caaacgctat atatgatgcc atcggtgtaa
     6421 ggatgactga attacctatg aatccagccg cagtaagaaa agctataaac ggagaataat
     6481 ttggtgaaaa gaattttagt cactgggatg agcggattaa ttggaggtct cctcaaggac
     6541 catttgattt ctatcggcga atatcaactt tcggccctaa atcggagttt tgtcgaagga
     6601 atatctaccc attcagctga tatcgcagaa ttagaacaaa ttaggcccgc ttttgaaaac
     6661 atagatgtgg tagttcattt agccgcatac ctaggttcag acagttttga agagcaactt
     6721 ccaaccaaca taataggtac atataacgtc tttgaggctg ccagggcagc tggggttaaa
     6781 agagttgttt atgccagtag tggtagcact gtaagagggg tggagaacat ctacccatat
     6841 ggggagatcg cccaaggtaa ctacgaaaat gttaaaggac ctattccact tatcacgcat
     6901 caagacatac gacctggggg agtttacggg gccacgaaag tctggggaga ggctttagca
     6961 cgacactttt cagatactta cgatatgtct atcctttgtg ttcgaatagg ttcagttaca
     7021 aaagaaaata ttccttctga tgcccgtcaa aagtccatat atctaagcca taaagatgta
     7081 tgccaaatac tgcaattatc tatcgacgca ccaagtgaac ttaaatacga tgttttttta
     7141 gcgacatcga acaataggtg gtcgtaccga gatctagagc acccaagaaa gatattagga
     7201 tatgaacctt tgaactcagc agaggattta taaatgcctt tagttatagg aattacaggg
     7261 gcaatagctt caggcaaaag cacagcctgt cagattttag aaaatctcgg ggggacacat
     7321 tgcgacgccg ataaattggt tcacagaatg tacgatcctg gtaaaccggc ttttcaccga
     7381 atcgtagaat cctttggaga tgaagttgta ggaaccgatg gttttataga cagaaaacaa
     7441 cttgggtcca aagtgtttgg aaaccctgaa aaaatgtctg aactcaccac tgcaattggc
     7501 gatatcaaag ctgaagtgag cggggtaatg aagcagtgga gaaatactct cagcctccat
     7561 gaggtcggcc tcatggaggc tgttaatttc atagaagccg gatacggaat atatagcgac
     7621 ataacctggc tattcgccgt ggatgacgag atggcgataa aaagacttaa ggaaagaaac
     7681 agttttagtg aggaagaagc tacacagagg ctttcaagtc aaaaaaaatg gcaagatcgc
     7741 gcgcctgcct cagatattgt aacgatgaat aacgggtctt taaaagaatt aaaagagtcc
     7801 gtaacaaagc aatatgctga attaaccgac aactattcaa aaggtttact cccaccttca
     7861 aaatacataa attggtggga aaaacattca cctaaagatt aattaagacc ttaattccaa
     7921 ccgcttaaag gcttctgcaa tttctgcatt gaccttcggc ccatatactg aaaatctatt
     7981 tcttaatctc acgcggccct catctttgga cgaagacatt tggcttgcat gg
//
LOCUS       DASCNS010000276        13308 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002039872, whole genome shotgun sequence.
ACCESSION   DASCNS010000276 DASCNS010000000
VERSION     DASCNS010000276.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13308)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13308)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13308
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002039872"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="QF838_09775"
     CDS             <1..319
                     /locus_tag="QF838_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HJO61006.1"
                     /translation="DNDTIYTPIDTKPKKDITKPLKRIENIANNDEVVVLFDRYSEDW
                     GELAYVMAKGKAKLVSDEQENLQAMRLLENRYIQYRDQNYLPRGAKIIGIRVYDYTSW
                     GNL"
     gene            447..815
                     /locus_tag="QF838_09780"
     CDS             447..815
                     /locus_tag="QF838_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61007.1"
                     /translation="MRIYFCPECGGYSLGPATSCDECQTDLPEDSWAEITEEELQQLE
                     YVEDFDLPHGISTWEYDVVRLKTDTEEGGLRYTTGLLKRMGDKGWELVNITPLGDKDG
                     PRYGIFKRIWDEGHGDEGDS"
     gene            complement(810..1370)
                     /locus_tag="QF838_09785"
     CDS             complement(810..1370)
                     /locus_tag="QF838_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HJO61008.1"
                     /translation="MKLYRGTKYWFIFLNYESFQMTMIEQIKYCQFCGTQLVRRYDRS
                     KERPFCQACNTFIYADPKVAVVAIVPSNKAILLIRRAIEPHLGKWSLPSGYVDRGEAI
                     EDALVREVFEETTIYTSPCHYLGVFSGKGPVVIHVYVATHKSGTAKAAEEVSEIKWCE
                     LDELPNLPFPYDQEIMSAYKTYLQSL"
     gene            1425..2642
                     /locus_tag="QF838_09790"
     CDS             1425..2642
                     /locus_tag="QF838_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013871.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61009.1"
                     /translation="MNIENLLEKTLTNPQYEAIRMLGDQFHKMGIRAFLVGGTVRDLL
                     LEREVSDIDIIVESDPSKLLNKFEPQYQLDVISNSQFNTCKIKFLNQIVDLAMARKEV
                     YPASGALPEVTSASIEDDLGRRDFSINSLAACINPSNWGEVLDLHKGLEDLHSKQVRI
                     LQSESFKNDPTRIFRCIRYSGRLGFSIENETQTQLLHDITDINLLSGVRITNELVKIF
                     SEKSLNSILDLMDQFEVSRHIHSGFPKGSGFPKRKKLLKNIRIPTLEFPLCVLISLIE
                     ADHRMDLIKRLDLGNKLAKFGADLNRIELIAADSSRSEIYKQLTDVSEITISVAKAYA
                     SKELRNNLDLFVNELKAINLCIDGEDLINIGIPQGPKIGNILAKILEDMIDSGQQSKS
                     GQIRMAKKLLCDS"
     gene            complement(2639..4057)
                     /locus_tag="QF838_09795"
     CDS             complement(2639..4057)
                     /locus_tag="QF838_09795"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HJO61010.1"
                     /translation="MSETQTFRSERDSMGEFQVPSEAYYGANTMRAVINFPISNLRFS
                     RSFIEAISMVKLAAAETNIELGLLDSKLGEAIIKSATEVSGGKFDDQFVVDIFQTGSG
                     TSTNMNANEVISNRAIEILGGELGSRYPVHPNDHVNTGQSSNDVIPTSIHLAAAKAIT
                     SDLIPSLEKLSASLNVKSQEFMPIIKTGRTHLQDATPVRLGQEFSGHAGQINRGIRRA
                     EHAVQELGEVALGGTAVGTGVNTHPEFAKRVCEKLTKSAGIEIRETSNHFQAQSTLDN
                     IVEASGAMKTIAVSLMKIANDVRWMGSGPRGGFGEIELPEVQPGSSIMPGKVNPVIPE
                     SVCQVAAQVIGNDMTVAIAGQSGNFEINVMMPVAAYNLLQSIDLLATASSNLAEQCIE
                     GLKATTKGPEMVEKGLAIVTTLVPHIGYDKSAELAHKAQQTGQTIMEVALVETDLNKQ
                     ELQKILDPESMTEPGVSDTSLG"
     gene            complement(4166..4810)
                     /locus_tag="QF838_09800"
     CDS             complement(4166..4810)
                     /locus_tag="QF838_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013866.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HJO61011.1"
                     /translation="MTDVTKELATLSTEISELESLLETPNQIKRPPCVILCHPHPRYG
                     GNMFDIVLNQISSRLLNSGIATLRFNQRGVGMSSGEPGEGTNELLDTNAVVNLTSSNP
                     KIDGSRLGIIGYSFGAWMALESALRSNLIKSLVSIACPQNKFAQYGTVQITQPKLLIL
                     GDRDHDFTLGQFKFLANRMSEPKKTEVITGADHFFREHSDLVGELSSEFIKETL"
     gene            4959..5987
                     /locus_tag="QF838_09805"
     CDS             4959..5987
                     /locus_tag="QF838_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HJO61012.1"
                     /translation="MTKVDLRSDTVTHPSPEMREAIAEAEIGDDVFNDDPSVNKLEEI
                     AAAKVGKEAAVFVASGTMGNLISVLSHCGRGDEIILGDKSHIFRSEAGGAAALGGVSY
                     HTVRNDARGMLDPSEVEEAIRDSTNSHNPHTAVIAIENTQNSCGGRVLTPQDTELIAN
                     VAHNYELPLHVDGARIFNAAVKLETPVDAMLKEADTVSFCLSKGLSCPVGSIVAGTHE
                     FVEEARRWRKMVGGGMRQAGFLAAAGIVALDQMVDRLAEDHANAKKLAEGLSKIDGVA
                     IDPDSVDTNLVFFEVEHPNKNELMKKLESEGIKGASPYSRWRFVTHYGVDSQDIDYVL
                     EVMADAMR"
     gene            6065..7267
                     /locus_tag="QF838_09810"
     CDS             6065..7267
                     /locus_tag="QF838_09810"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO61013.1"
                     /translation="MLLLFKNKSFRRFFYGLLFDDAAMMVSTMLQGWIVLIQTDSAFW
                     VGATAGVSGVGMTLFSPLAGVLIDRYNRKILLLLTQAIQIVLVAILGIAAFYELLEVW
                     HVLGYGFCAGAFHAVRLPCKMAMTLDLVGPKNLLKGTAANFFSMTVMGVLAPLAGGWL
                     IDREIYLGFALMLISLLVSSSIFLNLKNIKSPEISGSSHLEDLVYGAKYVAKNTLVRS
                     LILAILVTEIFGWAVESMLPVIARDELGLDARGLGVLMSVGALGAAISTVIMTAMPDV
                     ENKGKLVIYGLFGFGFGLIAFAFSDVLFLSMILLAFAYGSGVIYESGLSTLLQTSVPD
                     SMRGRVLSFQSLCWGFSGSAGFHAGVIAAILGAPIAVAAGGAVVIINGTRIAKNMISI
                     GAKVASGK"
     gene            complement(7275..8603)
                     /locus_tag="QF838_09815"
     CDS             complement(7275..8603)
                     /locus_tag="QF838_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HJO61014.1"
                     /translation="MNMFDFEASRHLTNNAPLAARMRPTSLDDIVGQAHLTGKNSPLR
                     RSIDAGRIPSFILWGPPGTGKTSLANLITSTSNFHLEKISAVTSGVSELRKIIAESRS
                     RLGMSGQRTALFIDEIHRFNKAQQDAILPHVEDGTVTFIGATTENPSFEIISPLLSRC
                     RVFTLNGLEKKDIVHILTNALNDSEKGLEKRNLSIEDGVLQDIAALSSGDARWALNTL
                     ELAADSTSIQGNEILISRSLLDEVIETKALPHDKAGDKHFDTISAFIKSVRASDPDAS
                     VYWLARLLLAGEDLMFIARRLVILASEDIGLADPRALTIAVSAQQAAHFVGMPEARII
                     LSEATIYLASAPKSNSAYKAINAAMSEIKSSGENPVPLHLRNPVTDLMSREKYGQGYK
                     YAHDYPEHFVRSENLPENLGSNDFYLPGNLGYEKFIAERLNKWWKEEYKE"
     gene            8832..8999
                     /gene="nrtS"
                     /locus_tag="QF838_09820"
     CDS             8832..8999
                     /gene="nrtS"
                     /locus_tag="QF838_09820"
                     /inference="COORDINATES: protein motif:HMM:NF038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HJO61015.1"
                     /translation="MAAGLVGTILILLNQGDFIVSGNFYRALIWKIPLTYIVPFCVAT
                     WGAVTNCKFID"
     gene            complement(8996..9298)
                     /locus_tag="QF838_09825"
     CDS             complement(8996..9298)
                     /locus_tag="QF838_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013844.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HJO61016.1"
                     /translation="MPYQNPQDSKLFKKLSIPNEIHLRKIKVAPAIERLESYIKDATN
                     AGMKEVKVIHGRAGTGDMRRAVHKFLSEHPDVERYYPAPPAEGGPGATIAVLELSF"
     gene            complement(9299..10690)
                     /gene="selA"
                     /locus_tag="QF838_09830"
     CDS             complement(9299..10690)
                     /gene="selA"
                     /locus_tag="QF838_09830"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HJO61017.1"
                     /translation="MIKQGQKMQEKLRHIPSVSAVLSDTRISELMTEYSTAAITDLVR
                     EKLSQVRSTIDDESEVPTISQISDHVSAHIEQNWANWPTNVINATGVVIHTNLGRAPL
                     STESIQATIDSSKTYSDLELELDSGKRGSRHARISQLLSSLTGAEAGLVVNNNAAALI
                     LGLSTLALGKEVIVSRSEAVEIGGGFRIPDVLKQSGATLIEVGTTNRTYVTDYEKELN
                     EETGAILSIHASNFKIIGFTASPSITELSILGAGNGIPILHDVGSGSLIDPTKYGLAP
                     EPLPQESIDAGTDLCFFSGDKLLGGPQAGIIVGRKTYIEQLSTHPLARAFRIDKMNLA
                     ALTATLIHYLKEEAQNKIPVWQMVSAELEDLHRRADKLAIIIPSSNLYSVKVIQTKST
                     IGGGSLPGETMDSYGIQMKVNNPEKFARKIRTGITPVIPRIEKDAVIFDLRTILKNED
                     EALIASITHTLQN"
     gene            10825..11424
                     /gene="coaE"
                     /locus_tag="QF838_09835"
     CDS             10825..11424
                     /gene="coaE"
                     /locus_tag="QF838_09835"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286762.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HJO61018.1"
                     /translation="MLSIGLTGGIGTGKSLVSNLLSDLGATVVNADLLGHEAYLPGTI
                     GFDLVVDAFGDKVVGDDGTVDRTKLGPIVFSSPENMAKLNSIMHPLIHDMIQTQLAEY
                     SSNGTDLVVVEAAVLIEANWQALFDEIWVVTSEREIVIERLKERNSLTREDAIARIDS
                     QMSQAERIEHSNVVVTNDGTTVELADNVKKIWNTRVVNK"
     gene            11442..12320
                     /locus_tag="QF838_09840"
     CDS             11442..12320
                     /locus_tag="QF838_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HJO61019.1"
                     /translation="MTTQSLYKSFIIEEYQLDEEPYYVPIGDEVELFEAAYKQKLPLI
                     FKGPTGCGKTRFVEYMSYKLGAELTKVTKNDADDSGVNLPLVTIACHEDLTGSDLVGR
                     YLLEGDQTVWLDGPLTRAVKAGGICYLDEVVEARKDTTVLIHPLTDHRRILPIEKRGE
                     LLQAADGFLLVLSYNPGYQSALKDLKHSTRQRFVSIEFDYPPMDLEAQIVEHEAGVDK
                     DTAYQLAKLGEKVRNLKEHGLGEGASTRLLIYAGQLIAQGIAPRRACQVSVNWAITDD
                     HSLQQSITEIISSIFE"
     gene            12361..12912
                     /locus_tag="QF838_09845"
     CDS             12361..12912
                     /locus_tag="QF838_09845"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HJO61020.1"
                     /translation="MSGIRELKNSSGNHIPAGVLVLLFDKNGEYWVILNRRTQKVEHH
                     KGEICFPGGRKDRLDKDLTETALRETWEEMGISPGSISVLGVLGKTETTTGYQITPTV
                     GLISYPYQFNIQNEEVEEIIEMPLSTLFSDDSKRDEAKILEGEVIMQPAFHYEDNMIF
                     GATARILNSFACLIDPRNLKEPA"
     gene            12909..>13308
                     /locus_tag="QF838_09850"
     CDS             12909..>13308
                     /locus_tag="QF838_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61021.1"
                     /translation="MTDKLKALEDLLPDLEKFPAPVKDNFNKAIPEMPDALSAEQTSD
                     WLKRGIAIAGQTVRSWEAAAHFFQVSPNVISSMPYSYFVRWMECGASLCEESPTLAAA
                     YFEAKFEVKCKALTIPFVSKIEWLPQGKKMK"
BASE COUNT         3728 a         2566 c         3005 g         4009 t
ORIGIN      
        1 tgataacgac acgatctata ctccaattga tacaaagcct aaaaaggata taactaaacc
       61 tcttaaaaga attgaaaaca tagcaaataa cgatgaagtt gttgtcttat ttgacagata
      121 ttcagaagat tggggtgaat tggcatacgt gatggctaaa ggtaaagcca aacttgtgag
      181 tgatgaacag gaaaacctac aggctatgag attacttgaa aatcgataca tacaataccg
      241 ggatcagaat tatcttccaa gaggggcaaa gattatcggg atcagagtat acgactatac
      301 atcttgggga aatctttaat aaatatattt ggatcttttt attttcaaga aaacgctaag
      361 atggtatcta tttcgcttat agtggacata taatcactgc aggttgatag tgttttcgaa
      421 tatacgaaag agaaaaataa taatctatga gaatttactt ttgtcctgag tgtggcggtt
      481 actcgttagg gccagcgaca tcatgtgacg aatgccaaac agacctgcca gaagactcct
      541 gggcagagat aacagaggaa gaacttcaac agttggagta tgtggaagat tttgatcttc
      601 cgcatggcat atcgacgtgg gagtacgatg tggtaaggct gaaaactgac acagaagaag
      661 gtggattacg ctacacaaca ggtcttctga aacgtatggg agacaagggt tgggagttgg
      721 tgaacattac tccgctaggc gacaaagatg gccctcgata cggtattttt aagaggatat
      781 gggatgaagg tcatggcgat gaaggtgatt catagacttt gtagataagt tttataggct
      841 gacattattt cctggtcata agggaatggt aagttaggta gttcgtccaa ttcacaccat
      901 tttatttcac ttacttcttc ggcagcttta gctgttcctg atttgtgagt tgctacatag
      961 acatggatca caaccggacc cttccctgaa aataccccta gatagtgaca cgggctggta
     1021 tatattgttg tttcctcaaa gacttcccgt accaaggcat cttctatagc ctcaccgcgg
     1081 tctacatagc ctgatggcag gctccatttg cctaaatggg gttctatggc tcgtcttatc
     1141 agcaatattg ctttgtttga tgggactatc gccactactg cgactttggg atccgcatat
     1201 atgaaagtat tacaagcctg gcagaatggc ctttccttgc tgcgatcgta tcttctcacc
     1261 agctgtgtac cgcaaaactg gcaatactta atttgctcaa tcatagtcat ttgaaagctt
     1321 tcataattta aaaatatgaa ccaatatttg gttcctctgt ataatttcaa agtataagac
     1381 ctgaaatctt actgtaaaga accaagagta atgtcgaaag atttatgaat atagaaaatc
     1441 ttttagaaaa aacgcttaca aaccctcagt acgaagcgat aagaatgctg ggggaccagt
     1501 ttcataaaat gggaatccgt gcttttttgg ttggaggaac cgtaagagat ttgcttctag
     1561 aaagggaagt ctcagacatc gatattatcg tggaatcaga cccttcgaaa ctattgaata
     1621 aatttgagcc acaatatcaa ttagatgtga tttctaactc gcagttcaac acctgcaaga
     1681 taaaattttt aaaccaaata gtggatctgg cgatggcaag aaaagaggta tatccagcct
     1741 ctggagcatt acctgaggta acttcagcct cgattgaaga tgatttaggg aggagagatt
     1801 tttcaataaa ttccttggca gcctgtatta atccttcaaa ctggggggaa gtcctcgatt
     1861 tgcataaggg gttagaagac ctacattcaa agcaggtaag aattttgcag tcagaatcct
     1921 ttaaaaatga cccgacgcga atatttagat gcatacgcta ctccgggagg ctggggtttt
     1981 ctattgaaaa tgaaacgcaa acccaattgc tgcacgacat aacagatata aatttgttaa
     2041 gtggggtacg cataaccaat gaattagtca agattttttc ggaaaagagc ctaaactcta
     2101 ttttggatct aatggaccaa tttgaagttt caagacatat tcactctgga tttccgaaag
     2161 gatcggggtt tcctaagaga aaaaaactgt tgaaaaatat caggattcct actttagaat
     2221 tccccctttg cgtgctgatt tcattaattg aagccgatca tagaatggat ttaatcaaga
     2281 gacttgattt aggaaataag ttggcaaaat ttggggcaga tttaaatcgc atcgaactta
     2341 tcgcagcaga ctcctcacgc agcgaaattt acaaacaact cacagatgtc tctgaaatta
     2401 caatatcagt agctaaagca tatgcctcaa aagagcttag aaataatctg gacctatttg
     2461 taaatgaatt aaaagctatt aatttgtgta tcgacggcga agaccttata aatattggta
     2521 tacctcaagg tccaaaaatt gggaatatat tggccaaaat tcttgaagat atgattgact
     2581 ctggccaaca gtctaagtct ggtcagattc gtatggctaa gaagctttta tgtgattctt
     2641 aacccaagga tgtgtctgaa actcctggtt ctgtcataga ttccgggtca agtattttct
     2701 gtaattcttg tttattcaaa tccgtttcaa caagagccac ttccatgatc gtctgaccgg
     2761 tttgttgggc tttatgtgct aattctgcag atttatcgta tccaatatgt ggaactaaag
     2821 tagtcactat agcaagacct ttttcaacca tctcgggacc tttggtggtc gccttaagtc
     2881 cttctataca ttgctcagcc aggtttgagg aggccgttgc taaaagatcg atagattgta
     2941 aaagattata ggctgctaca ggcatcatta cgttgatttc aaaatttcct gattggccag
     3001 ctattgcaac agtcatatcg tttcctatga cctgagctgc tacttgacac actgactcag
     3061 gtatgactgg gttgacttta cctggcatta tagaactgcc cggttgtact tctggaagtt
     3121 caatttcacc aaatcctccc cgaggccctg aacccatcca tctcacatcg ttggctattt
     3181 tcatcaaact cactgcgatt gttttcatcg caccactggc ttctactata ttgtctaagg
     3241 tactttgagc ttggaaatga ttggatgttt ctctaatttc tatcccagcg gatttcgtca
     3301 atttttcgca gactctttta gcaaactctg gatgtgtgtt tactcctgtt ccaaccgctg
     3361 tgccacctaa ggcgacttcc ccgagttctt gaacagcgtg ttctgcgcgc cgaatgcctc
     3421 tatttatttg tcctgcatgt ccggaaaatt cctgaccgag acgaacagga gtcgcgtcct
     3481 gtagatgcgt tcttcccgtt ttaattatcg gcatgaattc ttgagatttg acattgaggg
     3541 aggcggataa tttttctaaa gatggaatta aatcacttgt tatagctttg gcggcagcca
     3601 aatgtatcga tgttggtatg acatcgttcg aagattgacc ggtatttacg tgatcgttag
     3661 ggtgtacggg ataccgggaa cccagttcac caccgagtat ttcaattgcc ctatttgaaa
     3721 ttacttcatt agcattcata tttgtcgaag ttcccgaacc agtttgaaat atgtccacga
     3781 caaattggtc atcaaatttg ccgcctgata cctctgttgc ggattttatg atggcttccc
     3841 ctaattttga atctaacaat cctagttcaa tatttgtttc ggcagcagct aatttcacca
     3901 tagatattgc ttcgatgaaa gagcgggaga aacgaagatt acttattgga aaattaatca
     3961 ctgctctcat cgtattagca ccataataag cttccgacgg tacttggaat tctcccatgg
     4021 agtctcgttc gcttctaaat gtttgggttt ctgacaattg actactccaa agtttgctgg
     4081 ttgaaatttt aggccgttat ttcttgattc caactacggc atcaagtaaa tgataattta
     4141 ttaacctttg catacctata ttagcctata aagtttcctt aataaattcg gaactcaact
     4201 cgccgactaa atcactgtgt tctctaaaga aatgatctgc tccggttatc acttctgtct
     4261 tcttcggttc tgacattctg ttagctaaaa acttaaattg tccaagagta aaatcatgat
     4321 ccctgtcacc gagtatcagt aactttggtt gagttatttg gactgtgcca tattgagcga
     4381 acttattttg tgggcatgca attgatacaa gagatttgat taaattactt ctcaaggctg
     4441 attctaatgc catccaggct ccgaaagaat agccaataat tcctaggcgg ctgccatcaa
     4501 tttttggatt agaacttgtc agattaacta ccgcatttgt atctagcaat tcattggtgc
     4561 cttccccggg ttctccgctg ctcattccga cacctctttg gttaaaccgt agtgtagcta
     4621 ttccgctatt cagtaaacgg ctggaaattt ggttcaggac gatatcgaac atattacctc
     4681 catatcgagg atgtggatgg caaaggataa cgcaaggagg tcttttgatt tggttaggtg
     4741 tttccaaaag ggattcaagt tcggaaattt cggttgataa tgtcgctaat tctttggtta
     4801 catcagtcat ataattaccc tgagaaaata ataatggcca taaacagtga tcaattcaaa
     4861 gttaaaaata agcataagta tagtaattaa gaaaagccgt tgctagaatc aatatataaa
     4921 agtaaaagtc cgaattaatt taccttggtg aaagttatat gacgaaagtt gatcttagaa
     4981 gcgacacagt tacccatcca tcccctgaaa tgagggaagc catagccgag gcggaaatcg
     5041 gggatgatgt atttaatgat gatccgtcag ttaacaaact tgaagaaatt gcggcagcga
     5101 aagtaggaaa agaagctgct gtgtttgttg caagcgggac gatgggcaac ctaatttctg
     5161 tcctatctca ttgcggaaga ggcgacgaaa ttatccttgg tgacaaatct cacattttcc
     5221 gttcagaagc cggcggcgct gcagctctcg gaggagtttc gtaccataca gttaggaatg
     5281 atgcccgcgg aatgcttgat ccttctgaag ttgaagaggc cataagagat tcaacgaata
     5341 gccataatcc ccacactgca gttatagcta tagagaacac tcaaaacagc tgtggtggta
     5401 gggtacttac tccgcaagat accgaactga tcgcaaatgt agcccataac tatgagctgc
     5461 cacttcatgt ggacggggct aggatattca atgcggcagt caaactggaa acccctgttg
     5521 acgcaatgtt gaaggaggcc gacacggtga gtttttgttt gtcaaaagga cttagttgtc
     5581 ctgtgggctc gatcgtggca gggactcacg aattcgtcga agaggctaga agatggagga
     5641 aaatggttgg tggggggatg aggcaagctg gctttctcgc cgcggccggt atcgttgcct
     5701 tagaccagat ggttgatcgt ttggctgagg accatgccaa cgcaaagaaa ttggcagaag
     5761 gattatccaa aatagatggt gtagcgatag acccagactc ggttgacacg aatttagttt
     5821 tctttgaagt cgaacaccct aataagaatg aattgatgaa aaaattagag tcggaaggaa
     5881 taaaaggagc ttcaccgtat tcccggtgga ggttcgtgac ccactacggg gtggattccc
     5941 aagatataga ttatgttttg gaagttatgg ccgatgctat gagatgataa cctcatcgtt
     6001 tgtgccggca tcacaccatt gcctttaata ttgaccctat agcgttacca ggcgcgtttt
     6061 atttatgctc ctcctgttta aaaataagtc ttttcggcgt tttttctatg gtctcttgtt
     6121 cgacgacgcc gccatgatgg taagtactat gctccaaggt tggattgttt taattcagac
     6181 agattccgcg ttctgggttg gggctactgc gggagtttca ggggttggga tgacattatt
     6241 tagccccctg gccggagttt taatagaccg ttacaacaga aaaatattgt tgttgcttac
     6301 acaggctatt caaatagttc tagtcgccat tttagggata gcagcttttt atgaactact
     6361 agaggtatgg cacgtgttgg ggtatgggtt ctgtgcaggg gctttccatg ctgtaaggct
     6421 tccttgcaaa atggctatga ctttagattt agtaggccct aaaaatctcc ttaagggaac
     6481 tgcagctaac ttcttttcta tgacagttat gggagtttta gcaccgttgg caggtggatg
     6541 gcttatcgat cgggaaattt atttgggatt tgccttaatg ctcatatcct tgcttgtatc
     6601 gagttcaatt ttcttaaatc tcaagaatat aaaatctcca gaaatatctg gctcgtctca
     6661 tttggaggat ttagtttatg gtgcaaagta tgtggccaaa aacacactag ttcgttcttt
     6721 gatacttgca attttggtca cagaaatttt tggatgggcc gttgaatcta tgttgccagt
     6781 aatcgcaaga gatgaacttg gattagatgc tcgaggatta ggggttctca tgtcagtagg
     6841 ggcgttagga gccgcaatat ctacggtaat catgactgca atgccagatg tagaaaataa
     6901 ggggaagctg gtgatttatg gactgtttgg atttggtttt ggattgattg cttttgcatt
     6961 ttctgatgtt ttatttttat cgatgatttt actggctttt gcctacggtt ctggggtgat
     7021 ttacgaaagt gggctatcca cactacttca aacatcggtt ccggatagta tgagaggtag
     7081 agttttaagt tttcaatctt tatgttgggg gtttagtggg tcagcaggat ttcacgccgg
     7141 ggtgattgcc gcaattttgg gtgccccaat tgctgtagcg gcaggtggag cagtggtgat
     7201 aattaatggg acgcgaatag ctaaaaatat gatatccata ggagccaagg tggcttcggg
     7261 gaagtgattg actgttattc cttgtattcc tctttccacc atttgtttag ccgttctgct
     7321 ataaattttt cataacccaa attccccggc aagtaaaaat cgttgctccc aagattttct
     7381 gggagatttt ctgatctgac aaaatgttct ggataatcat gggcatactt gtaaccttgg
     7441 ccgtatttct cccgtgacat taaatcagtc acaggattgc gtaaatgaag tgggactgga
     7501 ttttctcctg aagatttaat ttcggacatt gcagcattta tcgctttata ggcagagtta
     7561 cttttaggag cggaggctag gtatattgtt gcctctgata gtatgattcg agcttctggc
     7621 attcccacga aatgggcagc ttgttgagcg gaaacagcaa ttgtcagtgc tctgggatca
     7681 gctaatccta tgtcttcact agccaatata acaagccttc tggctatgaa catgaggtct
     7741 tccccggcca agagtagcct ggctaaccaa tacacagaag cgtcagggtc tgaggctcgg
     7801 acagatttta taaacgcaga tatcgtgtca aagtgtttgt ctcccgcttt atcatgcgga
     7861 agagccttcg tttctattac ctcatccaac aatgatctac ttattaatat ttcattgcct
     7921 tggatagagg tagagtctgc tgccagttct aatgtgttca aggcccatct ggcatcccca
     7981 ctggacaatg ccgctatatc ttggagcact ccgtcttcaa tggaaagatt tcttttttct
     8041 aaacctttct ctgaatcatt cagagcattg gttaatatgt ggacgatgtc tttcttttca
     8101 aggccattta atgtgaacac tctacatcgt gataaaagag gagaaattat ttcgaaggac
     8161 ggattttctg tggtggcgcc tatgaaagtg acagtgccat cttctacatg tggcagtatt
     8221 gcatcttgtt gtgctttatt aaatcgatga atttcgtcaa taaaaagagc agtcctttgc
     8281 cctgacattc ctagccggga tctagattcg gcaattattt ttctcaactc agacactcca
     8341 gaagtgacag cactgatttt ttcgagatgg aaattcgaag ttgaagttat aaggtttgct
     8401 agggaggttt taccggtccc cggtggcccc cataaaataa atgaagggat cctccctgcg
     8461 tcgatacttc ttcggagggg gctatttttg ccagttaaat gagcctgacc tactatgtca
     8521 tcaagactgg ttggccgcat tctcgctgcc aaaggggcgt tattggtcag gtgtcggctc
     8581 gcctcaaaat caaacatgtt catcgtttta gcctcgatgg atgtgtaaaa atggtttggc
     8641 tcacgtaatg caatagtgta caagaatcag cgagttattg attggagact aagatgcaga
     8701 aatccttgat ttgtggcagg tgtaaagaaa cgataaacgg ttcaacttat ttagtcagtc
     8761 caaatggttc gtctgggtat cggcactttt ggtgtttatt ggcgtatttt ccaatgttaa
     8821 accgctcctt aatggctgca ggcctggttg gaacaatatt aattctgttg aaccagggcg
     8881 attttatagt ttctggtaat ttctataggg ccctaatttg gaagatacca cttacatata
     8941 tagtgccatt ctgcgtggcc acttgggggg ctgttaccaa ctgtaaattt atagattaga
     9001 acgataattc caatacagct attgtggctc cgggccctcc ttcggcaggt ggggctggat
     9061 agtatctttc aacgtcggga tgttcagaca ggaatttatg tacggctctt ctcatatcac
     9121 ctgtaccagc gcgcccgtga atcaccttga cctctttcat tccagcattc gtcgcatctt
     9181 tgatgtagga ttctagcctt tcaattgcag gagccacttt gatttttcga agatgaatct
     9241 catttggaat agacaacttt ttgaagagtt ttgagtcctg cggattctga taaggcaatt
     9301 aattttgcag tgtatgggtt atggatgcta tcagggcctc gtcttcattt ttcaatatag
     9361 tccgaagatc gaatattact gcatcctttt caattctagg aattactggt gttataccag
     9421 ttctaatttt gcgggcgaat ttttcaggat tgttcacttt catctgaatt ccatatgaat
     9481 ccatggtttc tcccggtagg ctaccgccac caatcgtaga ttttgtttgg atgactttca
     9541 ccgagtataa gttactacta ggaattatga tcgccagttt atcagctctt ctgtgtaagt
     9601 cctctaattc tgcagatacc atctgccaga caggaatttt gttttgggct tcttctttta
     9661 agtaatgaat taaggtggca gtcaaggcag ccagattcat tttatctatt ctaaaagctc
     9721 tcgctaaagg atgggttgat aattgctcga tatatgtttt tcggcccaca atgattcctg
     9781 cctgcggacc acctaacagt ttgtctccgg aaaaaaaaca taaatcggtg ccagcatcta
     9841 tgctttcttg tggtagaggt tctggtgcca ggccgtattt ggtcggatca atcaatgatc
     9901 cacttcccac atcatgtaag ataggaatcc cattcccagc tcctaaaatt gaaagctctg
     9961 taatcgaagg gctggcggtg aagccgatta tcttgaaatt acttgcatgg atggaaagga
    10021 tagccccagt ttcctcattt aattcctttt catagtccgt cacataagtt ctattggtcg
    10081 tcccaacttc aattagggtt gcgccacttt gctttagaac atccggtatt cggaaaccgc
    10141 ctccaatttc cacagcttca gatcgtgaaa ctattacctc ttttcctaac gccaatgttg
    10201 agagcccaag tattaaagcc gcagcattat tgttgaccac taaccctgct tcagctccag
    10261 tcagactaga gagcagttgg gaaattctcg cgtgcctaga tccgcgcttt ccagaatcta
    10321 gttctaattc cagatcactg taagttttgg aagaatcaat agttgcttga atactttcag
    10381 tacttagggg tgcacgccct aggttagtat gaataacaac tccggtcgca tttattacgt
    10441 ttgttggcca attggcccag ttttgttcta tgtgggctga gacgtggtca gatatttgcg
    10501 aaattgttgg aacttctgac tcatcatcaa tagttgacct tacctgactc agcttttcac
    10561 gtacgagatc tgttatagct gctgtcgagt attcggtcat caattcggaa attcgggtgt
    10621 ctgatagtac ggcactgaca ctggggatat gtcgcagttt ttcttgcatt ttttgaccct
    10681 gtttgatcat ttagcctccc attatatatt gttgaagaaa tcgacttcta ttctcttaat
    10741 ttttcttgaa aacacggttt taattgaccc tcgaaaatct cctcctgtag aatgaccgaa
    10801 tgactttttc ctagacgagg taatatgctt agtattggtc ttaccggagg gatagggact
    10861 ggcaagtcgc tagtttctaa tctattgagc gatcttggtg caacggtggt gaacgccgac
    10921 ttgcttggcc atgaggcata ccttccgggg actatcgggt ttgatttggt cgttgatgcc
    10981 ttcggagata aggtagtggg agatgatggt actgtcgaca ggaccaaact cggtcctata
    11041 gttttctcca gcccagaaaa tatggcgaag ctaaattcaa tcatgcatcc attgattcac
    11101 gatatgatcc aaacccagtt ggccgaatac tcttccaatg gaactgattt ggtcgttgtg
    11161 gaagctgcgg tgctgataga agccaactgg caagccctgt ttgatgaaat atgggttgtt
    11221 acctcagaaa gagaaatagt aattgaaaga ttaaaagaga gaaattcttt gactagggag
    11281 gatgcaattg ccaggataga ttctcaaatg tcacaggctg aaagaattga acattcaaat
    11341 gttgttgtta ccaatgatgg aacgacagtt gaattagccg acaatgtaaa aaaaatttgg
    11401 aacactagag tagttaataa ataggagtgg aatagctgca catgacaact caatcactat
    11461 ataaaagttt cattattgaa gagtatcaat tagatgaaga gccgtactac gtgccaattg
    11521 gggatgaggt ggaattgttt gaggcagctt ataagcaaaa gcttcctctc atattcaagg
    11581 gacctacggg atgtgggaaa actaggtttg ttgaatatat gtcatataaa cttggtgctg
    11641 aattgaccaa ggttaccaaa aatgatgcag atgattcagg ggtgaattta cctctagtaa
    11701 caatagcttg tcatgaagac cttaccggaa gtgatctcgt tggtagatat ttattggaag
    11761 gagatcagac cgtctggctt gacgggccgt tgactagggc tgtgaaagcg ggagggatct
    11821 gctacctcga cgaagtggtg gaggcaagga aagatactac agttttaata catccattga
    11881 ctgaccatag aagaatctta cctattgaaa aaagaggaga gcttttgcag gcggctgacg
    11941 gattcttgtt ggttttatct tataaccccg ggtaccaaag cgctctaaaa gacctgaagc
    12001 acagcactag gcaaaggttt gtttccattg agtttgatta tccgccgatg gatttagagg
    12061 cccaaattgt cgaacatgaa gccggagtag ataaggatac agcttaccaa cttgccaaat
    12121 taggagagaa agttaggaac ctaaaagagc acgggctagg tgaaggagcc agtacaagac
    12181 ttctgatata tgccggccag cttatagctc aaggtattgc acctcgtaga gcctgccaag
    12241 tatcagttaa ttgggcaata acagacgatc attccttaca gcagagcatt actgagataa
    12301 tttcttcaat ctttgaataa acgctcagtg acgctatcac ctgcctacat agagaatgta
    12361 ttgagtggaa taagagaact gaaaaattct tcaggaaatc atatacctgc gggtgttctg
    12421 gtgttgcttt ttgataagaa tggtgaatat tgggtgatat tgaataggag aactcaaaaa
    12481 gtcgaacacc ataaagggga aatttgtttc cctggaggta ggaaggatag gttggataaa
    12541 gatttgactg agactgcttt gagagaaact tgggaagaaa tgggtatctc cccaggcagt
    12601 atttcagttc taggagtatt gggcaaaact gagactacga cgggatatca aataactcct
    12661 acagttgggc taatatccta cccatatcaa tttaatattc aaaacgagga agtagaagaa
    12721 atcatagaga tgccactgtc tactctcttt tcagacgata gtaagagaga tgaggcaaaa
    12781 atactagaag gcgaagtgat catgcagccg gccttccatt atgaagataa catgatcttt
    12841 ggtgcgacag ctaggattct caatagtttt gcctgtctca tcgatccaag aaatttgaag
    12901 gagcctgcgt gactgataaa ttaaaagcac tagaagattt actgccagac ctagaaaaat
    12961 ttccagctcc tgtaaaggac aattttaata aagccatccc tgaaatgccg gacgcacttt
    13021 ctgctgagca aacatcagat tggttaaaaa gaggaattgc tatagcaggg caaacggtgc
    13081 gttcttggga ggccgccgct cacttctttc aagtaagtcc caatgtgatt tcttcaatgc
    13141 catacagtta ttttgtacga tggatggagt gtggggcttc actgtgtgag gagtctccga
    13201 ccttggcggc agcatatttc gaagcaaagt ttgaagtcaa gtgcaaagcc ctgactattc
    13261 cttttgtatc taaaattgaa tggcttcccc aggggaagaa gatgaaaa
//
LOCUS       DASCNS010000277         3441 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002042695, whole genome shotgun sequence.
ACCESSION   DASCNS010000277 DASCNS010000000
VERSION     DASCNS010000277.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002042695"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="QF838_09855"
     CDS             complement(<1..1037)
                     /locus_tag="QF838_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJO61022.1"
                     /translation="MGEITGEQIIAKALKTQGVEHGFGVVGIPVGGIAGAMMKEGMTY
                     HGMRHEMPATYAAQAVSYLSGNGGIGVALAVSGPGVLNAVGAYANAWSNRWPMLLLGG
                     SYERSGHMQGFFQETDQLTAMRPYSKFAERVEDPYRLPIYIAEAIKKATHGTPGPAYL
                     DLPGDVIQNSWDEDKVIWAPKVISPRKTLSDPADVEAAIKALKTAKQPLVIFGKGVNN
                     ADAASEMREFVERTGIPYLAMPMAKGIIPDDHPQSAAAGRSFVLQNADLVVLVGARMN
                     WMLHFGLPPRFREDVRIIQLDFNPEEIGVNVATEVALIGDAKLTLGQMIDVLHRDQWQ
                     FPQDSEWLNEVG"
     gene            1376..2608
                     /locus_tag="QF838_09860"
     CDS             1376..2608
                     /locus_tag="QF838_09860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO61023.1"
                     /translation="MLSKSDTTQFGSTKAWIFVGILAGGHTLYHWVVQGFIVVLPEVQ
                     HHFFLNSIGVGLILTVREVSTGLVSLPGGILSDALKQHWAVFLTVCIGGFGAGAVLLG
                     TSPAFPLLLAGIAIIAASHSFWHLSAASTLSRSFPNDRGTVLSFHGIGGSIGDAGGPF
                     VTGLLLYLLGWQKILSFYGVIAIGFAFVFLFSVSKILKPSESKKDQINYARHISTVFR
                     ALKQPVIWGIAAVKGLRGMSLVALLTILPLYFYDDLSMGPSSRGLHIGLLIVVGIITK
                     PLAGYLSDRFGRKQILIPGLIWSSLIALSLVFFSQGIFLAISIVLLGVFLYPDQPIIT
                     AATLDIAGENISSSVLGVTGTISLAMSTMSPLMAGAIYQNFGFDMVMVYLGILFALAA
                     ALTFTLPLNVNITDGSKL"
     gene            2785..>3441
                     /locus_tag="QF838_09865"
     CDS             2785..>3441
                     /locus_tag="QF838_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013755.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61024.1"
                     /translation="MIQYIADIEQACIALKGLVSESEIGLDIETTGLDPILDSIRLVQ
                     IATPLKTYVIDMYHVPTSVLNPLLTGGPIKVIHNSKFDAGFFYQAPDGVMVEPIFDTM
                     ITDQVLHHRGYARSLKDLSKEYLDIDLDKEKQTSDWGVEHLSEAQIQYAAQDADVLLP
                     LKHIQEEQAMELQITSVVNLENRLVPAICWMERSGVSLDRHLWDELVKSAEAKAVQLK
                     E"
BASE COUNT          890 a          803 c          727 g         1021 t
ORIGIN      
        1 cccacttcat tcaaccattc tgaatcctgt gggaattgcc attgatctct gtgcaataca
       61 tcaatcatct gacctagagt gagtttggcg tcacctatca atgcaacctc agtcgccaca
      121 tttactccga tttcctctgg gttgaaatct aactgaatta ttctcacatc ttctctgaat
      181 cgtggcggaa gtccgaagtg caacatccaa ttcattcgag ctcctactaa cacaaccaag
      241 tcggcattct gtagcacgaa tgaacgaccg gcagccgcgg attgaggatg atcatctggg
      301 ataatgcctt tcgccatagg catagcgaga taaggtatcc cagttctttc aacaaactct
      361 ctcatctcac tggccgcatc agcgttattc acgcccttgc cgaaaataac cagtggttgc
      421 ttagcagttt ttaaagcctt aattgcggcc tcaacatcag ccggatcaga taaggttttc
      481 ctaggactga tcaccttggg ggcccaaatt actttgtctt cgtcccagct attttgtata
      541 acatcacctg ggagatccag atatgccgga ccaggagtac catgagtcgc cttttttatt
      601 gcctcagcaa tatagatcgg taatctgtat gggtcttcca cacgttcagc aaatttcgag
      661 tagggtcgca tagcagtcag ctgatcagtt tcctggaaga atccctgcat gtgcccagat
      721 ctttcataag atccaccaag tagtaacatc ggccatcgat ttgaccatgc gtttgcatag
      781 gcgcctacgg cattcagcac acctggtcct gatactgcaa gcgcgactcc gattccccca
      841 ttcccactca ggtaagacac tgcctgcgca gcatatgtgg ctggcatctc gtggcgcatt
      901 ccgtggtagg tcattccttc cttcatcatg gcaccagcaa tacccccaac tgggatacct
      961 acaaccccaa atccatgctc cacgccttgc gtcttcagtg ccttcgcgat aatttgttct
     1021 ccggtgatct cgcccatgat tcacactcct tcctgatttt taaaatgaac taatttgagc
     1081 agtacgtagc tgcgtcttta ccagtgtgag gatgttacac cattcggatt taatgctcat
     1141 tttttctatg gaattttaac tgaatatcaa taaaaattct ttcctataaa ctcgttattc
     1201 tttgatatcc ctttgtgcgt aacccattta tctaccccaa acagtgcgaa ttcctatcac
     1261 accatgtgtt agtcgaaatg ttactattcg cccatagaaa tttgctgact aatatttata
     1321 ccaattaacc ctttatttat cggggtgctc cacctaatgt tgttgattta ataagatgct
     1381 atctaaatcg gatactactc agtttggcag cacaaaagca tggatttttg tggggatatt
     1441 agccggtggg cacacacttt accattgggt ggttcaagga ttcattgtgg tgctccctga
     1501 ggttcaacat catttttttt tgaacagtat tggggtaggg ttgattctta cggtaaggga
     1561 agtgtcaaca ggattagtgt ccttacccgg aggaatactg tctgatgctc tgaagcaaca
     1621 ctgggctgtc tttctgactg tttgtatagg aggattcggg gctggggcag tcctattggg
     1681 gacatctcca gcatttccac tacttttagc tggcattgcc atcattgccg cttcacattc
     1741 cttttggcac ttgtctgcgg cttctacttt atctcggagt tttccaaacg ataggggaac
     1801 tgtcttgtca ttccatggga ttggtggaag catcggagat gcaggtggtc catttgttac
     1861 cggcctactt ttatatcttc taggttggca gaaaatcctc agcttttatg gagtcatagc
     1921 aattggattt gccttcgtat ttttattttc tgtatccaag atattgaaac caagtgaatc
     1981 taaaaaagac cagatcaatt atgcgcgcca tattagcacg gtattccgtg ccctaaagca
     2041 acctgttata tggggtatag ctgccgttaa aggtctccgc ggcatgtccc tagtagcact
     2101 actgacaata ctgcctttat atttttacga tgacctatct atggggcctt cttcaagagg
     2161 gttacatata ggactgctaa ttgtagtagg aattatcaca aaacccctgg ctggatacct
     2221 gtcagacagg tttggaagaa aacagatttt aattcctggg ttaatttggt ctagtttgat
     2281 tgccctatcc ttagtgtttt tttcacaagg gatttttcta gccatatcaa tagtgctcct
     2341 tggagtgttt ttatacccag atcaaccgat cataactgcg gcaaccctgg atatcgcagg
     2401 ggaaaacata tcttctagcg tcctcggtgt aactggaact ataagtttag ctatgagtac
     2461 gatgtctcca ctaatggcgg gagctatcta ccaaaatttc gggttcgata tggtcatggt
     2521 ttatcttgga attctttttg ccctagcagc agctcttacc tttaccttac cgttaaacgt
     2581 gaatattact gatggatcga aactgtaacc accctatata gagatactac cctgtcggaa
     2641 attctcattg gaaagcatgc tacgtgacac tccttcgtgc gtaccccatt tatcttcccc
     2701 caacagcacg atttccaatc acaccatgtg gtagtcaaaa tgttactatt cgaccatgct
     2761 catcaaaatc atcggaacta gcccttgatt cagtatatag cggatataga acaggcctgt
     2821 attgctttaa aaggacttgt gtctgagagt gaaataggct tggatataga gactacagga
     2881 cttgatccaa tacttgacag cattaggctt gtccagattg caactcccct caagacatat
     2941 gtaatcgata tgtatcatgt cccgaccagc gtgctgaacc ccctcctcac tggtggtccg
     3001 ataaaggtga ttcacaattc caagttcgat gcgggatttt tctaccaggc tcccgatggg
     3061 gtaatggttg aaccaatttt tgacacgatg atcaccgatc aagtgttgca ccatcgtgga
     3121 tatgccagga gtctgaagga cctgtcaaaa gaatatctgg atatagacct ggataaggaa
     3181 aaacaaactt cagactgggg agtcgaacac ttatcggagg cacagattca gtatgcagcg
     3241 caggatgccg acgttttact tccactgaag catattcagg aagagcaggc aatggagctt
     3301 caaataacgt cagttgtaaa ccttgagaat cgattagtac cagcgatctg ctggatggaa
     3361 agatctggcg ttagtttaga cagacatctt tgggatgaac tggtcaaatc agccgaggcc
     3421 aaagctgttc aactcaaaga a
//
LOCUS       DASCNS010000278         1069 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002049467, whole genome shotgun sequence.
ACCESSION   DASCNS010000278 DASCNS010000000
VERSION     DASCNS010000278.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002049467"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1069
                     /gene="purD"
                     /locus_tag="QF838_09870"
     CDS             <1..>1069
                     /gene="purD"
                     /locus_tag="QF838_09870"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HJO61025.1"
                     /translation="NFALAREIDLTIVGPEAPLALGIVDVFVKSGLPIFGPTASASKL
                     ESSKSFAKDVMEKWDIPTASHKVFRDSESANSYLESLNSFPTVLKADGLAAGKGVYIC
                     KDQSEAISALKDVMVTKVFGDAGTTLVIEEFLVGWEISVFAFTDGESISDLVAACDYK
                     RIGEGDTGPNTGGMGAFSPPRVWDQNLKNEIRDLVISPIVEAMRTDHEPFIGTIFAGM
                     MITAEGPKVVEFNCRLGDPETQAILPLLRTDILEIFESCVNRRLNSIKVDWRKDASVA
                     VVLASGGYPSTYTTGYPIDGLALDRDSVNVFHAGTKLDGEGKIITNGGRVLAVNALAP
                     DINQAAEVVYENIREITFSNMY"
BASE COUNT          309 a          176 c          278 g          306 t
ORIGIN      
        1 aattttgcct tggcgagaga aattgattta actatagtcg ggccagaagc tcccttggct
       61 ttgggcatag tggatgtttt tgtgaagtca ggacttccta tttttggacc aacagcttct
      121 gcctccaagt tggaatccag taagtctttc gcaaaagatg ttatggaaaa atgggatatc
      181 ccaaccgcct cacacaaagt ttttcgggat tctgagagtg ctaattcata cctggaaagc
      241 ctgaattctt ttcctacggt tttaaaggcc gacggattgg cagcaggtaa aggtgtatat
      301 atttgcaaag atcaatccga agcgatatct gcacttaaag atgtaatggt tacaaaggtg
      361 tttggtgatg caggaacaac attggtgata gaagagtttc tagtcggttg ggagataagt
      421 gtgtttgcat ttacggatgg agaatctatt tctgatctag ttgctgcttg tgattacaaa
      481 aggatcgggg aaggagatac aggacccaat acaggaggta tgggggcatt ttctccacct
      541 cgggtttggg accaaaactt aaagaatgaa attagagatt tggtgataag tccaattgtt
      601 gaagcaatga gaactgacca tgagcctttc attgggacaa tatttgcggg catgatgatt
      661 acagcggaag gtcccaaagt tgtggagttc aattgtaggt tgggagatcc ggaaactcag
      721 gctattttgc cactccttag aactgatatt ttggagatat tcgaaagctg tgtgaataga
      781 cgattgaact ccattaaggt tgactggaga aaagatgcga gtgttgctgt agtattggca
      841 tctggtggat atccttcgac atacacgacg ggttacccaa tcgatgggct ggcattagat
      901 agagattcgg taaatgtatt ccatgctgga acaaagctag acggggaagg taaaattatt
      961 actaatggag ggcgggtact tgctgttaat gctttggcac cagatatcaa tcaagcagct
     1021 gaagtagtgt acgaaaatat ccgtgaaatc acctttagca atatgtact
//
LOCUS       DASCNS010000279         6420 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002054513, whole genome shotgun sequence.
ACCESSION   DASCNS010000279 DASCNS010000000
VERSION     DASCNS010000279.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002054513"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="QF838_09875"
     CDS             <1..772
                     /locus_tag="QF838_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HJO61026.1"
                     /translation="RTVEDCALTLSAIAGFDQKDQYMRDLPVPDYCAALTGDIKGVKI
                     GVVREFLDPDVMGVTKSVRQGVLQATEVLATLGAEVEEISIPLAPVSGVASRIISAVE
                     RSSLHPEWLRNRPNEFHHNTRVAFTTGELIPSQVYYKAQKVRTLVRKQALEALERFDV
                     LVMPGASEPATVMNLEPGIRSKEAAVRALTEASYRGLFSLVSGPALSICCGYTVEGDN
                     KLPLGIQIAGRPFDEAMVMNVAYAYEQNTNWHLEYPSL"
     gene            complement(960..1308)
                     /gene="ssrA"
                     /locus_tag="QF838_09880"
     tmRNA           complement(960..1308)
                     /gene="ssrA"
                     /locus_tag="QF838_09880"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1462..2241
                     /gene="hyi"
                     /locus_tag="QF838_09885"
     CDS             1462..2241
                     /gene="hyi"
                     /locus_tag="QF838_09885"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HJO61027.1"
                     /translation="MPNFAANLSMMFNEVDFIDRFRVASESGFNAVEYLFPYEYPKEQ
                     LVTLLGENNLVQVLHNLPAGDWNAGERGIACYPDRVNEFQEGVGLAAEYASALNCPQV
                     NCLAGIKPESVSNQEAQATLVANLRYAAPIFKEAGVKLIVESINTLDMPGFFLNRTDQ
                     ASSLINEVASDNLYLQHDIYHMQIMEGDLARTIESNIDLISHMQLADNPGRHEPGTGE
                     INYKYLFEFIDALGYQGWIGCEYIPAGTTVGGLGWITSYLK"
     gene            2312..4687
                     /locus_tag="QF838_09890"
     CDS             2312..4687
                     /locus_tag="QF838_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HJO61028.1"
                     /translation="MASDMDSVKSEPQLSIELLESVQDRVLWLAMQLVHHANNIRKNP
                     DGAKVGGHQASSASIVTLMTSLYFDHMNNQDRIAVKPHGSPVFHAIQYLLGNLDSSYL
                     TELRAYHGLQAYPSRTKDPDLVDFSTGSVGLGPVAPNFASIVRSYNNSHKHGDNPKGR
                     YISIVGDAELDEGSVWEAIAEPAMQDASDILWVVDLNRQSLDRVIPGIRVGAWKDMFV
                     ANGWHVVEAKYGALLEEAFQLPNGEMLREAIDSMSNQVYQRLLRVNSEELRAWLPSYT
                     AYPEEMKKFISGWNDEELNRLFHNLGGHDFSTLRKAFQETDKTKEPSVVFAYTLKGYR
                     LPSVGDPQNHSVTLSASQMEELRENLDIEPANIWGGFDSESEESHFLMSTSEKLRRLD
                     VVTPPPKEDIPSDFGRRYSGYMSTQQIFGLVLTDMSRSLGDLSRRIVTVSPDVASSTN
                     LGGWINRVGIWKLNETETLPEEGIVRALKWEESKDGQHIELGISENNLFMMLGQLGLS
                     NEMNGEMLFPIGTLYDPFIRRGLDAFVYSVYSGAKFIVVGTPSGLSLAPEGGAHQSMM
                     TASIGVEMPQLDAYEPCFGQELEWIMLSALNQIRKRGQSTYLRLTSKRVDQNLLQLPS
                     DTSEIDSLRLQVLKGAYCLRDRTTDSFSKGNGDRITILSVGAMIPEACEASDILLEEG
                     VLANVINVTGPGPLYRHYRDLVKESIGKGYGLSEGLATLFPGHIDGAPILTLADAHPH
                     SLSWIGGALGSRTVSLGVDDWGQSGSREDLYQEYGIDVETIVNAALSTLEL"
     gene            4748..5176
                     /locus_tag="QF838_09895"
     CDS             4748..5176
                     /locus_tag="QF838_09895"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HJO61029.1"
                     /translation="MMKILVVHGAGINMRGKSQVEIFGSDTMEDYSNQIKEYAKQLDV
                     TVDIYHSNIEGEVINKFYQAHEDGYDAAIINPAGYSSGHPALAAAITQVEYPTIEVHI
                     SNPSARGNASQIAPSCKGVITGFGLFGYYIALKAIKNIAG"
     gene            5202..5894
                     /locus_tag="QF838_09900"
     CDS             5202..5894
                     /locus_tag="QF838_09900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJO61030.1"
                     /translation="MRFSIPIAVPASVLILMALFLIYVSCESSQESETSQESSEVKPY
                     ANPPAMTIDTTKDFFAVFEMEDGGRFTIDLYEKLAPNTVNNFVFLANENYYNGTTFHR
                     VIDGFMAQGGDPSGSGSGSPGYYFENELHVDARHDSAGVLSMANKGLVDGKGTNGSQF
                     FITFSATEFLDGFDANDQPKDCASDSCHSVFGKVLTGMDVVTGISKRDPSSATEPGDA
                     IKTVTITTRESN"
     gene            complement(5891..>6420)
                     /locus_tag="QF838_09905"
     CDS             complement(5891..>6420)
                     /locus_tag="QF838_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61031.1"
                     /translation="TENLILESRVIPHSAAQPYSNVPYQPESNAPLPSTNPSKTKTPL
                     GIIIPVAIVAIILIIIAIYRNSLPKPYGFVSDESGTSVLNFDRLQKRRLIRSIFARHI
                     ISGSETGMAEFVGTDFVFGKGSLLITATSAAHTIRVNNHPLVGSAYVVDGTWIGATGK
                     LFRFTKERTYPDHSS"
BASE COUNT         1832 a         1207 c         1568 g         1813 t
ORIGIN      
        1 tcgtacggtt gaggattgtg cattgacctt gagcgccatt gctggatttg atcaaaaaga
       61 tcagtatatg agagatttac cagttccaga ttattgtgct gcactgactg gggatataaa
      121 gggagttaaa ataggggtag tgagggaatt cttggaccct gatgtcatgg gagttactaa
      181 atcggtgagg caaggagtgt tacaggcgac agaagtgctt gctacccttg gggctgaagt
      241 agaagagata tcgatcccat tagcccccgt ctctggtgtc gcaagccgga taataagtgc
      301 agttgaacgc tcaagtttgc acccagaatg gcttcggaac agaccaaatg agtttcatca
      361 taatactcga gttgccttta ctactgggga attgattccg tcacaggttt actacaaggc
      421 acagaaggta cgtaccttgg tgcgcaaaca ggctttggaa gcacttgaac gatttgacgt
      481 gctggtaatg cccggtgcct cagaaccggc tacagtcatg aatctagaac ctggaataag
      541 aagtaaagag gcggcagttc gtgctttaac tgaggcatca tatcgagggt tgtttagtct
      601 agtaagcggc ccagctctgt ctatctgctg tggatataca gtagaaggag ataacaaact
      661 tcctttggga atccagattg caggccggcc gtttgatgaa gctatggtca tgaatgttgc
      721 ctatgcgtat gaacaaaata caaattggca ccttgagtac ccttcattgt gaattgacta
      781 agaatgtagt gttaaacctt ggaaatgcct aggcttacaa tttttgaaga tcattggcag
      841 atcactggaa gatcactgtt ggattagagt tgattgtatt cgctgcagca atactgctgt
      901 tggatccgca aatgctcaaa gcaggttctc aataaatttg atgaaggcaa ttgcgtccat
      961 ggtggagacg ggggagggtc gaactccccg tccagaagac accccgaccg agatatacta
     1021 caggtttagc cggacattaa attcgactgc tcaagccctg accggctagc gcttgaaaca
     1081 gactagccga tagtctttag ccagatatat cggcatctaa ctgactgcac cccgtctatt
     1141 gtcgcccttt cactaccttc ggggatcgga cgtgaaggac gtagccactt aagcggctag
     1201 tgctagctgt ttgttgccag ttactttttt gccatctttt taacgagggt gaaggcgccc
     1261 cgacctgcaa tctcgcggtg acatcccctg tcgaatccgc gcgtccccat gtggtagcaa
     1321 attctatcat gattgggttc ctcggatact ttgtttggag ttattatgat tgaataggac
     1381 gcgatgttta acttggaaag tagatataca atctagacat cccaatatgc aaactttaaa
     1441 tcaaataaat aaggagaaga aatgccaaat tttgcagcta atctcagcat gatgttcaac
     1501 gaggtagatt ttattgatag gtttagagtt gcctctgagt ctggatttaa cgcagtggaa
     1561 tatctattcc catatgagta tcccaaggaa caattggtga ctcttttagg ggaaaataat
     1621 ttggtccaag tcttgcataa tttgccagct ggtgattgga atgcgggaga aagagggatt
     1681 gcttgctacc ctgatagggt gaacgaattc caagaaggtg ttgggttagc tgcggaatat
     1741 gcctcggctt taaattgccc tcaggtgaat tgtttagctg gaataaaacc agaatcagtg
     1801 tcgaaccaag aagctcaagc tactttggtc gccaacctca ggtatgcggc gcctattttc
     1861 aaagaagccg gtgtgaagct tattgtggaa agcataaaca ctttggatat gcctggcttt
     1921 tttctcaatc gaacggacca ggcctcttca ttaattaacg aggttgcctc tgacaattta
     1981 tatttgcagc acgatattta ccatatgcaa attatggaag gagatttggc aagaacgata
     2041 gagtcaaata ttgacctgat aagccatatg caattggcgg ataatcctgg ccggcacgaa
     2101 cctggaactg gggaaataaa ttacaaatat ttatttgaat ttatagatgc ccttggatac
     2161 caaggatgga taggatgcga atatattcct gcaggtacta ctgttggcgg tctaggttgg
     2221 ataacttcct acctgaaata agggacttta gataccgaat agaactacca atgctatact
     2281 tttgtaacaa gcactccagg ggcaatgaat aatggcaagt gatatggatt cagttaaaag
     2341 tgaaccccaa ctttcaatag agcttttgga gtcggtgcag gaccgtgtgc tttggttggc
     2401 aatgcaattg gttcaccatg ctaacaacat tagaaaaaac cctgatgggg caaaagtcgg
     2461 cgggcatcaa gcctccagcg catcaatagt tacattgatg acctctctgt actttgacca
     2521 catgaataat caggacagaa ttgctgtaaa accccacgga tctccagttt ttcatgcaat
     2581 tcagtacctt ttagggaacc ttgattcatc atatctcact gaattgagag cctatcacgg
     2641 tcttcaggca taccctagta gaaccaaaga tccagatcta gttgattttt caacaggttc
     2701 ggtaggccta gggccggtag ctccaaattt tgcctcgata gtacgctctt acaacaattc
     2761 ccataagcat ggagataacc ctaaaggaag atacatatcg atagttggag atgctgaatt
     2821 agatgaaggt tctgtttggg aggcaatagc cgaacccgct atgcaggatg cttcagatat
     2881 tttgtgggtt gttgatttaa atcggcaaag tctagataga gttatccctg gcataagagt
     2941 tggcgcatgg aaagatatgt ttgtggctaa tgggtggcat gtagtggagg ctaaatacgg
     3001 agctctgctg gaggaggctt ttcagctacc aaatggcgaa atgctgcggg aagcaataga
     3061 tagcatgagt aatcaggtct atcagcgtct tttgagagta aattcagaag aactgcgggc
     3121 atggttgcct tcttataccg cctatccaga agaaatgaag aaattcatat ctggctggaa
     3181 tgatgaagaa ctaaaccgcc ttttccataa cttgggaggt cacgacttct cgacccttag
     3241 gaaggctttc caagagacag ataaaactaa ggagccaagt gtagtatttg cctatacgtt
     3301 gaaaggatac aggttgccat ctgtcgggga tcctcagaat cattcggtta cattgtcagc
     3361 ttctcaaatg gaagagctca gagaaaattt agatatcgaa ccagccaata tatggggcgg
     3421 gtttgatagt gaatctgaag aatctcactt tctcatgtct acgtcggaaa aactgcgaag
     3481 acttgatgtg gtaacccctc cccccaaaga agacattcct tcggattttg gcaggaggta
     3541 ttctggatat atgtcgacac aacaaatttt tgggttagtg ctaactgata tgtctagaag
     3601 tttgggagac ttatctagaa gaatagttac agtgagccct gatgtagcaa gttccacgaa
     3661 tttaggcggt tggataaata gagtcggtat ctggaagctt aatgaaactg agactcttcc
     3721 agaagagggt attgtaagag ctttaaaatg ggaagaatcc aaggatggac aacatattga
     3781 attgggtatc tctgagaata atttgttcat gatgctaggc cagttagggc tttccaatga
     3841 aatgaatggg gaaatgttat ttcctatagg gactctctac gatccattta taaggagagg
     3901 gttggatgca tttgtgtata gcgtttactc aggtgctaag ttcattgttg tagggacacc
     3961 gtccggtttg agtttggccc ctgaaggtgg tgcacatcaa tctatgatga ctgcatctat
     4021 tggggttgaa atgccccagt tggatgcata tgaaccttgc tttggtcaag agctggagtg
     4081 gatcatgtta tctgccttaa atcagataag gaaaagagga cagtcaactt atttgagact
     4141 cacaagcaaa cgagtcgatc aaaatttatt gcaacttcca tcggatactt cggaaattga
     4201 ctcgttgaga ttgcaggttt tgaaaggagc ttattgtttg agagaccgga cgacggattc
     4261 cttttcaaaa gggaatggag atcgcataac aatcctaagt gttggagcca tgattccgga
     4321 ggcatgtgag gctagtgaca ttctgcttga ggaaggcgtc ttagctaatg tcattaatgt
     4381 gaccggcccc ggccctttgt atcgacatta tagagattta gtaaaggaat ctattggaaa
     4441 aggttatggc ttgtctgaag gtttggcaac gttgtttcca ggtcatatag atggcgcacc
     4501 gattttgact ttggcagatg cgcatccaca ttccctttcc tggattgggg gagccctcgg
     4561 tagcagaaca gtgtcgttag gtgttgatga ttgggggcag tctggtagta gagaagatct
     4621 ttaccaagaa tatggaattg atgtcgaaac aatcgtaaat gctgccttat ctactttaga
     4681 actttgattt gcacgctgtg ctagaatttg gcactattct gagagaattc catttggaga
     4741 aatattgatg atgaaaatat tagttgttca tggtgcgggg attaatatgc ggggtaagtc
     4801 acaggtggaa attttcggaa gtgacacaat ggaagactat agtaaccaaa ttaaagaata
     4861 tgctaaacaa ctggatgtaa ccgttgatat ttaccactca aatatcgagg gggaagtcat
     4921 aaataagttt taccaggctc atgaggatgg ctacgatgca gctattatta acccggccgg
     4981 ttattcatcg ggccatccag cattggcagc tgctataact caagtagagt atccaacaat
     5041 cgaagtccat atttccaatc cctcggcaag aggtaatgct tcccagatag caccttcctg
     5101 caaaggtgtt atcactggat ttggattatt tggatactat atagccttga aagcgatcaa
     5161 aaacatcgct gggtaatgtt aaaggggtag atctacaaaa aatgagattt tcaataccaa
     5221 ttgcggtacc ggcctcagta ttaatattga tggcattgtt tcttatatat gtttcttgcg
     5281 aaagtagtca ggaaagcgaa actagtcagg aaagttctga agtgaaaccg tatgctaacc
     5341 cacctgccat gactatagat actactaaag acttttttgc cgtgtttgaa atggaagatg
     5401 gtgggcgatt caccatagat ctatatgaaa aactcgcacc taatacggtt aataattttg
     5461 tatttcttgc aaacgaaaat tactataatg gtacgacctt ccatagagtg atcgatggct
     5521 ttatggcaca ggggggcgat ccttccggta gtggtagtgg tagccctggt tattactttg
     5581 agaacgaatt acatgtagat gccaggcatg attctgcagg agttttgtct atggctaata
     5641 aagggctagt ggacgggaaa gggaccaatg gtagccagtt ttttataact ttttcagcaa
     5701 ctgaattcct agatggattt gacgcgaatg accaacctaa ggattgtgcc tcagattcct
     5761 gtcattcagt gtttggcaag gtgttgactg ggatggatgt tgtgaccggg atatctaaac
     5821 gggatcctag ttctgccacc gaacctggcg atgctattaa aactgtgacg ataacgacga
     5881 gagaatctaa ctaggaacta tgatcggggt aagttctctc ttttgtgaat cggaataatt
     5941 tacctgttgc tccaatccaa gtgccatcta caacgtatgc tgacccaacc aaagggtgat
     6001 tgtttaccct aattgtatgt gcagcacttg ttgcggttat caaaagagag ccctttccga
     6061 atacgaaatc ggttccaaca aactcagcca ttccagtttc cgaaccgctg attatgtgtc
     6121 gggcaaaaat tgaccttatt aatcttcttt tttgtaaacg gtcgaaattt aacactgagg
     6181 ttcccgactc atcactaacg aacccatacg gctttggcag agaatttcga taaatagcta
     6241 ttattattaa tataatagct acaattgcca caggaatgat gattcctaat ggggttttgg
     6301 tttttgaagg gtttgtgctt gggagagggg catttgattc aggctgatag ggcacattgg
     6361 aatatggttg tgcagctgaa tggggaatca cccttgattc caaaatcaaa ttttctgttg
//
LOCUS       DASCNS010000280         2579 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002060173, whole genome shotgun sequence.
ACCESSION   DASCNS010000280 DASCNS010000000
VERSION     DASCNS010000280.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002060173"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="QF838_09910"
     CDS             complement(<1..559)
                     /locus_tag="QF838_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HJO61032.1"
                     /translation="MKSRQVGYVLIALIVIGIAGLFYRIVIGGTDEFILEGLLPISED
                     IINQVDIKTNDGLASELLKVNDSYWEVANNPIFTPKLAAFWEHVDDVSGAQLVARKTK
                     YHQLLGVDDESSTKVSFYVGPSLQEQFHIGKWSPEVRLCYVRKSGKNEVYSIPCSQNG
                     IFSSDPDSWRNPIVISIPPSDVTSFD"
     gene            complement(559..>2579)
                     /locus_tag="QF838_09915"
     CDS             complement(559..>2579)
                     /locus_tag="QF838_09915"
                     /inference="COORDINATES: protein motif:HMM:NF021351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HJO61033.1"
                     /translation="TQPIRGWIVLLAKFTSGLLFVSIAIVATIGIPLSLTSAGDLDWG
                     ASISQYIGSIFLAGCLVSIGLFTSSLTRNQIVSFILGLTVSMALMIMGLEIVAITLPS
                     TAANLLQLLSPITHFDNIGRGIIDFRDILYFLSLISTFLCGTFMVMRSRTLSHKTPQY
                     RNLQLGVAGFIILSILIGWFGTSIKGRLDLTEDKIFTLSPATEEIVGQLDDLLTIDVY
                     MSKDPPVQVSPVSRDINDFLDDVESSSDGMVKIARHFPEDDEKALKKADNAGIPPIQF
                     NVRSQGELQIKTGYMGLALTYLDKREVVPFIQSVDGFEYRLASLVNKMLDDRSQKKIV
                     AFLSGHQEMEISRELASFASVLNQQYQVITLATNEGIPIDLEDVDVLMITSPKSRIGE
                     THRESIKNYITDGGKAFIAIEPVLVDFNQLAGIPNRDSSADFILSTFGISVESDLVFD
                     LQSNETLAFGTGGAGNVYLPYPYWVKANVVDSKIGGEVSNVVMPWASTVGILDLPDSE
                     YTVSPLISTTEFGSIDMNLRNLRPNSPTFSETTGDNFVLSDLGVALEHSNGSRMVITG
                     DADWISETATRFNSNVLLGLNIIDWLAQEDNLASVRSKVVSERNLVFSSDRHRNIVQY
                     SNIGGVPLVFIILGLARFMQRRSKGFRNRWSVGSNAKSESPQQNEVSD"
BASE COUNT          737 a          560 c          500 g          782 t
ORIGIN      
        1 agtcaaaaga cgttacatct gacggaggta ttgatattac aataggattt ctccaagaat
       61 cggggtccga ggaaaaaatc ccattttgag agcaaggtat cgaatacact tcatttttcc
      121 cagattttct cacgtagcag agccttacct ccggcgacca tttcccaata tgaaattgct
      181 cttgcaagga ggggcctaca tagaatgaaa ccttagtact gctttcgtca tctacaccta
      241 acagctggtg gtatttagtt tttcttgcaa ctaattgtgc tcctgacaca tcgtcaacat
      301 gttcccaaaa ggcagctaat tttggggtga aaattggatt atttgccact tcccaataag
      361 agtcattgac ctttagcaat tcagacgcta acccatcatt tgttttaata tcgacctgat
      421 ttataatatc ctcactgatg ggtaaaagtc cttctaagat aaattcatcg gtaccgccta
      481 tgacaatcct gtaaaacagt cccgcaattc ctataactat caatgctatt agtacatatc
      541 cgacttgcct acttttcatt aatctgaaac ctcgttctgt tgagggcttt cacttttggc
      601 atttgatcct actgaccacc tatttctaaa tcctttgctt ctacgttgca tgaacctggc
      661 aagccctaaa atgataaaga caagcgggac tcctcctatg tttgagtact gcactatatt
      721 tctgtgtctg tcagaggaaa ataccaagtt acgttcagaa accacctttg acctgactga
      781 agctaaatta tcctcttggg caagccagtc gatgatgttg agtcctaata ggacgttact
      841 gttaaaacga gttgcagttt cggagatcca atctgcatct cctgtgatga ccattctgga
      901 accgttgctg tgttctaaag ccacgcctaa gtcacttaga acaaaattat cccctgtggt
      961 ctcagaaaat gtgggggaat tcggtctcag atttctaaga ttcatgtcaa tcgaaccaaa
     1021 ttcagtggtt gaaatcaatg ggctcacagt gtattcggag tcaggtaggt ctaggatacc
     1081 gacagtactg gcccaaggca ttaccacatt actgacttcg ccaccaattt tactgtcaac
     1141 aacgtttgct tttacccaat acgggtaggg caggtaaaca tttccagctc ccccagttcc
     1201 aaaggctaaa gtttcattcg actgaagatc gaaaactaaa tctgactcca cggatatacc
     1261 aaatgtcgat aatatgaaat ctgccgaact gtccctgttt ggtattcctg ctaattggtt
     1321 gaagtctacg aggacaggtt ctatagctat gaatgcctta cccccatcgg ttatataatt
     1381 ttttatcgat tctctgtgag tttctcctat tcgggatttg ggagacgtaa tcatgagtac
     1441 atccacatct tctaggtcta ttggtatacc ttcgttggta gccaaggtta ttacttggta
     1501 ttgttgattt aagactgagg caaaagatgc caattcccta gatatttcca tttcttgatg
     1561 gcctgataaa aacgcgacaa ttttcttttg ggatctatcg tcaagcatct tgttaactaa
     1621 actagccagc cggtattcaa atccatcaac actttgtatg aaagggacta cctcacgttt
     1681 gtctaggtag gttaatgcta accccatata acctgtctta atttgtaatt ctccttggga
     1741 tctcacattg aactgtatag ggggtatccc agcattatca gcttttttca aagctttttc
     1801 atcatcttct ggaaaatgac gtgctatctt gaccattcca tctgagctag actccacatc
     1861 gtctaaaaag tcattaatat ccctcgatac tggagaaacc tgtacgggag gatctttgga
     1921 catgtatacg tctattgtta atagatcatc caactggcct acgatttcct cagttgctgg
     1981 gcttaaggtg aaaattttat cttctgtaag gtctaatctg cctttaatcg aagttccgaa
     2041 ccaccctatg aggatgctaa gaatgataaa tccagctacc cctagttgta agtttcggta
     2101 ttgcggggtc ttatggctga gagtcctact tctcattacc atgaaagttc cgcaaaggaa
     2161 ggttgaaatc aatgataaaa agtaaagtat gtccctaaaa tctataatgc ctctcccaat
     2221 gttgtcgaag tgagttattg gactcagaag ttggagtaaa ttcgcagcag ttgaaggtag
     2281 tgttattgcc acgatttcta gacccataat catgagggcc atacttacgg ttaggcctag
     2341 tatgaaagag acgatttgat ttcttgtaag actggaagtg aataatccta tagaaaccaa
     2401 gcaaccagcc agaaaaatgc tgccaatata ttgggaaatc gaggcacccc aatctaaatc
     2461 tccagcactg gttaaagata ggggtatgcc tatggtagct acgatagcta tcgacacaaa
     2521 taatagtcct gatgtgaact tcgctaacag cacaatccaa cctctaatag gctgagtta
//
LOCUS       DASCNS010000281         1457 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002066796, whole genome shotgun sequence.
ACCESSION   DASCNS010000281 DASCNS010000000
VERSION     DASCNS010000281.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002066796"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="QF838_09920"
     CDS             complement(<1..295)
                     /locus_tag="QF838_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61034.1"
                     /translation="MFGRIIEWFKDQYGGSREHLSRYEQVQRQFAEGSGWRNNKFVLY
                     ILILAASSLVCFGGFLAFEKGIFFLGAIVSGVLLVLGTVGKYFHSEVLDEHSYK"
     gene            complement(834..907)
                     /locus_tag="QF838_09925"
     tRNA            complement(834..907)
                     /locus_tag="QF838_09925"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(873..875),aa:Gln,seq:ctg)
     gene            1041..>1457
                     /locus_tag="QF838_09930"
     CDS             1041..>1457
                     /locus_tag="QF838_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO61035.1"
                     /translation="MYKIDLSGKTAVVFGIANQRSIAWSITKILSDAGAEIVAAYQNE
                     RVKEPVSKLISELPNVSMIECDVSDDKNVESAFSQIKSISGKVDIVVHSIAFAQKEDL
                     GGRFVDTEREGFRVALDVSAYSLVSISRHASQLMTEG"
BASE COUNT          425 a          302 c          327 g          403 t
ORIGIN      
        1 ctttgtaaga atgttcatca agcacttcgc tatgaaaata tttccccaca gtgccaagaa
       61 ccaataggac tccagaaaca atagctccaa ggaagaatat tcccttctca aacgccagaa
      121 atccgccaaa gcagactaag gatgaggcag cgagtattaa gatatacagt acgaatttat
      181 tatttcgcca gccagaaccc tcggcaaatt gcctttggac ttgttcatat ctagatagat
      241 gctctctaga gcctccatac tggtctttga accactctat gattctgcca aacataagca
      301 tacttccccc gccattatag gtaccttcgt attatgtgcc catgaaaaag atagtgatgg
      361 ctctcaccca tttccctatt ttccatttga gttaaatact caactagtat aggagcggct
      421 cccacaaagg cggggttgag tctgattgtt cgtccgcctg gagagggcgg tgtcctaggc
      481 tgctagacga atacgccaag tgtaaaaacg gtttttcacc gctagatttt aaatcagtat
      541 tcgggttcat gttcacaaaa gtattcccgg tctttttcat aaatacctag tctccgtcgg
      601 cgtttttctt cgatcagtgc cgcctgaaat taggcttgga ttagcctcta taagaagagt
      661 ggctaattta tatttgcctt ttattaattc caaggtttgt gggattgtag gttcccggag
      721 tcgaagtagc gacagcggaa tctcatgcta aacccatagt gggaaagtgg tgatttcagc
      781 cgtgagagga gggtgacatg gctagctata caaatgcgat acggttaatt tcctggctgg
      841 ggatatagga atcgaaccta tgcttacaga ttcagagtct gttgtcctac cgctagacga
      901 atccccagta ttacacaaat tttaacaagg caatataagc gacacaaggt aagcacatgg
      961 catctaacct gttgccgagc gggtagggta gaccataaaa ttcaatcata aggtagaaaa
     1021 tatctgagct agaggtccta atgtacaaaa tagatctgag tggtaaaacc gccgttgttt
     1081 ttggaattgc aaatcaaagg agtatagcct ggtctataac taagattctc tctgacgcgg
     1141 gagcagaaat agtcgctgca tatcaaaatg aaagagtcaa agaaccagtt tcaaagttaa
     1201 tatccgagct tccgaatgta tcgatgattg aatgtgacgt tagcgatgac aaaaacgttg
     1261 aatcggcttt ttcccaaatc aagtccattt caggaaaagt ggacattgtt gtccattcaa
     1321 tagcgtttgc tcagaaagaa gatttgggcg gtaggtttgt ggataccgaa cgtgaggggt
     1381 ttagggtggc actagatgtt agcgcctact ctcttgtgtc aatatccagg catgcttccc
     1441 aactaatgac tgaaggt
//
LOCUS       DASCNS010000282         2894 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002071221, whole genome shotgun sequence.
ACCESSION   DASCNS010000282 DASCNS010000000
VERSION     DASCNS010000282.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002071221"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="QF838_09935"
     CDS             <1..484
                     /locus_tag="QF838_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HJO61036.1"
                     /translation="AETREQAREQAKEGLFRHNNEYSTRALRPDDGPIYKTPDEAVDA
                     TAFSGVGNAVIGTPDDLINKIKEMVDLTGGFGAVIGFAHDWANRENTKNSWDLVARYV
                     VPEVNSMLLDYRDSIEFVVNNRETWARATDARLSNVADHKSASKIMETEGLEGEKSGS
                     "
     gene            complement(501..1046)
                     /locus_tag="QF838_09940"
     CDS             complement(501..1046)
                     /locus_tag="QF838_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61037.1"
                     /translation="MTISALIVLVLRIILPLSIFKNRITGAILSMTLDMLDVVIVDAL
                     QVALGEPKVGFGDNYQLFDKWLDMYYLSIEALVCLSWPNRLAKVTGISLFLFRLAGIS
                     AFEITGEEFRKLIFFFPNLFENFFLYYIICERFAPRLIPTRIKSMMIVLLILYLPKFG
                     QEYVLHFAEVKPWQWVKTNIF"
     gene            1346..1999
                     /locus_tag="QF838_09945"
     CDS             1346..1999
                     /locus_tag="QF838_09945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJO61038.1"
                     /translation="MSLRIGDKAPNFTANTTQGQINFHNWLGNQWGVFMSHPADFTPV
                     CTTEIGRVANLKSEFTKRNTKVLVVSVGTKADLTPLESHNEWIKDVNETQNANVDFPL
                     VEDPNKEIADAYGMIHPNANDTLTVRSVFIVGPDKLVKLIISYPASTGRNFSEILRVL
                     DSLQLTANYQVATPADWEDGQDCIVVPAVATEDIPNKFPKGHTIVKEYLRTTPQPNL"
     gene            2013..2504
                     /locus_tag="QF838_09950"
     CDS             2013..2504
                     /locus_tag="QF838_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HJO61039.1"
                     /translation="MKEFFSFPELINEPAARLVASAVMVMSLISIGLIKLEENFVLII
                     LLVMAYGFLARVLFGPKVSPLALIVTKILVPKLNLEEKLVPGPPKRFAQSIGLVFSVS
                     VLTLWLIDLNAMSIVLLSILSVFAFLEAVIGYCFGCKVFKFLISVGLVPEQICKRCAM
                     YEY"
     gene            2538..>2894
                     /locus_tag="QF838_09955"
     CDS             2538..>2894
                     /locus_tag="QF838_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61040.1"
                     /translation="MLETLASSRIPFMDRFRKVGTNGLGFHTTAEEATKSLDLQNKTI
                     VVTGANSGLGLETVRVLSLRGATVIGMARTIDKAKSIKSVMNKQFTPLECDLSEPSSI
                     TKSAENLIRSGIQIDSI"
BASE COUNT          940 a          574 c          598 g          782 t
ORIGIN      
        1 agcagaaacg cgcgaacaag ccagggaaca agcaaaagag ggattattca ggcataataa
       61 cgaatacagt actcgggcct taagacctga tgatggccca atctacaaaa ccccagatga
      121 agcagtagat gctactgcat tttcgggtgt aggtaatgca gttattggca ctcctgacga
      181 tttaattaat aaaataaaag aaatggtcga ccttacgggt gggtttggag cggttattgg
      241 attcgcacat gattgggcta acagggaaaa tacaaaaaat agctgggatt tagtcgcaag
      301 atatgttgtc cctgaagtca actctatgct gctcgattac cgggattcta tagaattcgt
      361 agtcaataat agagaaactt gggcacgagc cactgatgct aggcttagta atgtggctga
      421 tcataaaagc gcctctaaaa taatggaaac tgaagggtta gaaggagaaa aatctggatc
      481 ttagctaatc tcattatcct ttaaaagatg tttgttttta cccattgcca aggtttcact
      541 tccgcaaaat gcaaaacata ctcttggccg aactttggta gatagagaat taacaaaact
      601 atcatcatag atttgattct tgtcggtata aggcgaggag caaacctttc gcaaattatg
      661 taatacagga aaaaattttc aaaaagattt gggaagaaga aaataagttt tcgaaattct
      721 tcccctgtta tctcaaaagc agaaatccct gccagcctaa ataaaaagag tgaaattccc
      781 gtcacttttg caagccggtt gggccaactc aggcaaacca gggcctcgat cgacaaataa
      841 tacatatcaa gccatttgtc gaacagttga taattatcgc cgaacccgac tttcggttcc
      901 cctaacgcaa cttgcaaagc atccactatc acaacgtcca acatatctaa cgtcatcgat
      961 aatatcgcac cagtaattct atttttaaaa atggataatg ggagaattat cctcaaaacc
     1021 aatactatta aagccgaaat ggtcattatc gaaaacctct gacattaacc ttatatcaaa
     1081 atatggccct aaaacccaat aaccttaaat cactgcgcca gtataaaaag agataaatgg
     1141 tgctaataac ctctgacttg aaacccttga aaacttatta acaagtggtt aggattagta
     1201 ccgaaatgaa tgattcaata tgcacatgca gctgttcttg ttgtaccgac cgacgtggat
     1261 cggggtattg atcgttgatt gaataagact agttaatcaa gtaaaagacc ccgcaaaagc
     1321 ggggtttttt tattaggaga ataaaatgag tttaaggatt ggagacaaag cccctaattt
     1381 cacggcaaat acaacccagg gccaaataaa tttccataac tggcttggta accaatgggg
     1441 tgtattcatg tctcacccag ccgactttac tcctgtgtgc actaccgaaa tagggagagt
     1501 ggcgaaccta aaatcagaat tcactaagag gaacacaaaa gtgctggtag tgagtgttgg
     1561 tacaaaggcc gatttaactc ctctcgaatc acataatgaa tggataaaag acgtaaatga
     1621 aactcagaat gctaacgtcg acttccctct cgtagaagat cccaacaaag aaatagcgga
     1681 cgcctacggt atgatccatc cgaatgcaaa cgatactttg acagttaggt ccgttttcat
     1741 tgttgggccc gataaattag tgaaattgat aatctcatat cctgcatcta caggaagaaa
     1801 tttcagcgaa atcctgagag ttctagattc tctgcaactt actgccaact accaagtcgc
     1861 tacaccggct gactgggaag atgggcaaga ttgcatagtc gtaccagcag ttgccactga
     1921 agacattccc aataagtttc ccaagggaca cacaatagta aaagaatatc ttcgaactac
     1981 ccctcagcct aatttatagg gtttatgtac atatgaagga gtttttttcg ttcccagaac
     2041 tgatcaatga gccagcggca cgactggtcg cttctgctgt aatggtgatg tcccttatat
     2101 ctattggtct gattaagttg gaagaaaatt ttgttttgat aatcctcttg gtaatggctt
     2161 atggattttt agccagagtt ttatttggcc caaaagtaag cccacttgct ttaatcgtca
     2221 ccaagatttt ggttcccaaa ttgaatcttg aagaaaaatt agttcctggg ccacccaagc
     2281 gtttcgctca aagtattggg ttggtgtttt cagtgtccgt tttgacattg tggctgatcg
     2341 atctaaatgc tatgagtata gttttactct caattctgag tgtatttgca ttcctggagg
     2401 ccgtaatcgg ttactgtttc gggtgtaagg tctttaaatt tctaatttca gtaggccttg
     2461 tgccagaaca aatatgcaag agatgtgcga tgtatgaata ctaaagatca attagcgcaa
     2521 ccagcaaaga gcaataattg ttagaaacat tggctagttc acggattccg tttatggatc
     2581 gcttcagaaa ggttgggaca aacggtttgg gatttcatac aactgcagaa gaggcaacaa
     2641 agtccctgga cctccaaaat aaaacaatcg tggttactgg agccaattct ggcctagggc
     2701 tagaaacagt aagagtctta tccctcagag gggccacggt cattggaatg gctagaacaa
     2761 ttgacaaagc taagtcaata aaatcagtca tgaacaagca attcacacct ctggaatgcg
     2821 atctatcaga acctagttca atcaccaaat ctgctgaaaa tttgattagg tcaggcatcc
     2881 aaatagattc tata
//
LOCUS       DASCNS010000283         8281 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002080369, whole genome shotgun sequence.
ACCESSION   DASCNS010000283 DASCNS010000000
VERSION     DASCNS010000283.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002080369"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="QF838_09960"
     CDS             complement(<1..524)
                     /locus_tag="QF838_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61041.1"
                     /translation="MNGFEALVSKIERIAKNRPHIIIGITGFGGSGKSHLANRLRDHF
                     GINNNQIVRIDNLYGQNPKGPGIFDQSDWNLIEHILKDSSAGKRIRYQGKDHKGKLLH
                     FDEDLPRIVIFEGIRLLQPKFSQYFDMSVWIDCPQDFAIKRAKARDRSQGEDETTVAG
                     WDTDWGPKDKKYFDT"
     gene            complement(524..2821)
                     /locus_tag="QF838_09965"
     CDS             complement(524..2821)
                     /locus_tag="QF838_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024754.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HJO61042.1"
                     /translation="MNKNFLEQLNPAQRESVESVTGPVLIVAGPGSGKTRVITNRIAH
                     LVLNEKVSPYNIGAVTFTNKASREMKDRLVPLLGDEARRLTVGTFHSFCSVILRRSGE
                     YIGLPNNFVIYDDDDQIAAIKKSMKDVDVDPKQFNPRSVLSTISNSKSQLVNFQGFNT
                     QKSNYYEEVVGRIFERYEEILSQGVALDFDDLLLKTHQLLAESPTAAEIYQTRFHYFM
                     VDEFQDTNVAQYSIAKMITETSRNLCVVGDPDQSIYSWRNADIRNILSFQSDFPEAKI
                     LPLEQNYRSSQNILSAAKNVIAKNKQRVEKNLWTSNDKGSLISLDEAYDEEEEARKVI
                     KEIQRLSDNEEYSLSDFAVMYRVNAQSRSFEMGCQRFGVPYQIVGGIKFYHRREIKDI
                     TSYLRVVLNPDDDISIGRIINVPPRGIGQRTLSEIGRVARQTGKSLYESMKAISSGDE
                     VSHQLGNRAINSLNQFISCIDQIKNASSELDIMQLIDITMAETGYRKYINNDENAEER
                     LENIQEYRNSSREYSSVPALEGLVGFLEGISLISDIDTLEDDTDSITLITLHQSKGLE
                     FPIVFITGMEEGLLPHIRSLDSGSPEELEEERRLFYVGLTRAEQKLFLTRAFRRGFRG
                     SYEPTVPSRFLSDIPASSLINNLPKNKSSKPKPETPENILEFRRDQSRRRTPNQGTTN
                     TSNTDRSGPKPIRKKRTVNKINILKSKEGPKFQIGDKVIHGQFGNGIVMACDPSGDDL
                     QITVAFKDGGGIKKLLQSLAKLKKV"
     gene            complement(2837..3757)
                     /gene="speE"
                     /locus_tag="QF838_09970"
     CDS             complement(2837..3757)
                     /gene="speE"
                     /locus_tag="QF838_09970"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HJO61043.1"
                     /translation="MNISSPEWHIEPISAGLIQGERILSVLCDIQTEFQHVMIVDGEC
                     FGKSLILDGKTQSTELDEFVYHEGLVHPSMVSHTNPKNVLIVGGGEGATAREVLKYPG
                     VEKVTMVDIDQKLVELCKKFLPNHHMNSFSNPRLDLYYSDALEFVESLNNRFDVAIID
                     VPDPLEFGPAFQLFTVEFFDLLKQKLTMNGIMVIQAGATGPSFSEQCFTAVANTISQT
                     FSSCAGYEIFVPSFGSTWGFVTASDKINPAENTEAFIDEELARQGIHDLKMYDGLSHK
                     GMFQLPKYTREGLISETRVITKSNPIFTYQ"
     gene            complement(3754..4131)
                     /gene="speD"
                     /locus_tag="QF838_09975"
     CDS             complement(3754..4131)
                     /gene="speD"
                     /locus_tag="QF838_09975"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HJO61044.1"
                     /translation="MKSLGTQILLDLENCNSRLLDDIDFIKDVLKEAANSAGATIIGE
                     TFHKFKPVGVTGVVSIAESHICIHTWPEYLYASVDIFSCGEDFDLEKACKIISAKLES
                     GDSFSRIIERGIREDEEDSGDGK"
     gene            4290..5300
                     /locus_tag="QF838_09980"
     CDS             4290..5300
                     /locus_tag="QF838_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HJO61045.1"
                     /translation="MSTEINLRVLLKSRPKGFPTEDDFDVVESVIPRPQEGEILLQSL
                     WLSLDPYMRGRMNDAKSYAPSVNIGDPMVGGAVAKVVESRSPLFKAGDILEGRFGWQS
                     YALSNGLGVRKINESLAPLQTAVGVLGMPGLTAYFGFLDVCEPTPGDTVVVSAASGAV
                     GQIVGQIAKIMGCKVIGTAGTESKVSFIVDELGFDAGINYKNTNVGEALDELCPEGID
                     VYFDNVGGIVTDSVMDRINTGARIAICGQISQYNLESPDMAPRNLGILTRSQAKMQGF
                     LVFAYESRYDEGITRMASWIKDGKLKYREDVVVGLRNAPRAFIGMLNGENFGKTLVKV
                     SE"
     gene            5304..6404
                     /locus_tag="QF838_09985"
     CDS             5304..6404
                     /locus_tag="QF838_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HJO61046.1"
                     /translation="MPDSLTPALLKFREEFSEFLLENSGSSEEEFRELSLDRGYYRMA
                     QPIELGGRASSAMEMLIARETIAKSGILYPGAIIGPEPGLLSKADGLLKSNYLIPLLR
                     GEKKGAFAFTESNADLPTVAEWDDGHLVVSGEKSYVSGGHLSDFMSVVLTVDPEKVTD
                     KSGPAVLVIDSNSNGIKMGDPFYSLDGSSHVVVEFQSVNVDAANIIGEVGQGIPRALG
                     NIMQERIEQSATSVGLAMYALDIVTAHLKKPHRSGGTLSDFEGVRLRYADMRINTFAA
                     RSVLYRVGRLLGSEDSVVNEVTTAKIFCTEAASDVVDTAVQLVGGQALVSGHPLEDLY
                     RKIRSMRLAGGASDILRLNVSKGVFEFDSGIV"
     gene            complement(6437..7705)
                     /locus_tag="QF838_09990"
     CDS             complement(6437..7705)
                     /locus_tag="QF838_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61047.1"
                     /translation="MKYINMRVNGKRTLFTAIISILILISAKSDVHHFTQSDYAVGSH
                     AFSLVRWELRHLPLKWLHLLWDKYPGHKPNDAERNTLIKDYLATTLKLQKELVSLDNS
                     TTLNRLVDTTSMTQTTLKKKEIEYLRRKREALRGRAEEAIESSVSSAARNNNLGLPFG
                     LLIPPTDFRLGDPPHILITSRRDKIELTGSKLIKSDLEWSDRTRIEERAEQYENTSAL
                     VDDLAGLGTYPAIVSDKDELRQLMRTAAHEWLHNYWILKPLGRKMWSSHNMQILNETA
                     ADLAGNELGDEAFKALGNTTVNSYRYDNLNSGNTHLVKILRETRTGVEEMLENGEIEK
                     AEQYMDKQLWKLKLGGYNIRKLNQAYFAFRGNYAEGPASISPIGKELRELRGYFDNVG
                     DFIASISEIGDYRQFHYLLNLKRKQLFLDK"
     gene            complement(7717..>8281)
                     /locus_tag="QF838_09995"
     CDS             complement(7717..>8281)
                     /locus_tag="QF838_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013980.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HJO61048.1"
                     /translation="QNEQYINQKLNDIKNRNFDIKPSKISLPATTKVFDVTYLALETE
                     DFSLKEANETIILKEPKERVSLITNSKLLQQWLQIKAVSILEKAVETNSKKLNIPYNK
                     IKIGSRKSIWGSCSHKKNINLNRNLLFLEPSLIKYVVTHELCHIHEMNHSKQFWYLLN
                     EVLPGSQKLQRQLKSVANSSIPSWALI"
BASE COUNT         2199 a         1630 c         1750 g         2702 t
ORIGIN      
        1 gtgtcaaaat atttcttgtc tttgggcccc caatcagtat cccagccagc tacagtcgtt
       61 tcatcttctc cttgtgagcg atctctagcc ttcgcacgtt taatggcgaa atcttgagga
      121 cagtcaatcc atacactcat gtcaaaatat tgactaaact ttggctgaag tagtcttatt
      181 ccctcgaaaa taactattct cggtaaatct tcgtcaaaat gtaaaagctt ccccttatgg
      241 tcctttccct gatatctaat tcttttgcca gcacttgagt ctttaagaat atgttctatt
      301 agattccaat cactttgatc aaatatgcct ggtcctttag ggttttgtcc atataaatta
      361 tcaattcgaa caatctgatt gttattaata ccaaaatggt ccctcagcct atttgccaaa
      421 tgtgatttac ctgatcctcc aaaccccgta attcctataa ttatgtgcgg tctatttttt
      481 gcgattcgtt caattttaga aaccaatgct tcaaagccat tcattaaact tttttaagct
      541 ttgcaagact ttggagtaac tttttgatcc cgcctccatc tttgaaagcc accgtaattt
      601 gtaggtcatc tccagatgga tcacaggcca tgactatccc atttccgaat tgcccatgta
      661 tcaccttgtc accaatttga aatttaggac cctcttttga ctttaatatg tttatcttgt
      721 taactgttct tttctttcgt atcggctttg gtcccgaccg gtccgtgttc gatgtattag
      781 tggtaccttg attgggtgtt ctcctccttg attgatctct tctgaattca agaatgtttt
      841 ctggagtctc gggtttgggt ttagacgatt tatttttggg gaggttatta attagcgatg
      901 aggcggggat atcggataag aatcgagatg gtaccgtggg ttcatagctt cctctaaatc
      961 ctctgcgaaa tgcccgggtc aaaaataatt tttgttcggc tcttgtcagc cccacgtaaa
     1021 ataatcttcg ttcctcttct agttcctctg gacttcctga atcgagggat cgtatatgag
     1081 gcaggaggcc ctcttccata cccgtgatga aaacgatggg aaattctaga cctttagatt
     1141 ggtgcaaggt aattaatgtg attgagtcgg tatcatcttc caaagtatca atatcagata
     1201 tcaaactaat tccttctagg aaaccgacta atccctccag ggcaggtaca gaagaatact
     1261 ccctggaaga gtttctgtat tcctgtatgt tttctagacg ctcttccgcg ttttcatcgt
     1321 tgttaatata tttcctgtat ccggtttctg ccatggtgat atcgattaat tgcataatat
     1381 ctagctcgga ggaggcattt ttgatctgat cgatgcaaga tataaattgg ttcaaagaat
     1441 ttatagctct attacccaat tgatgggaaa cttcatctcc agagcttatg gccttcattg
     1501 actcatacag agatttgccg gtttgcctag ctactctgcc tatttctgaa agggttcttt
     1561 gtccaatacc ccttgggggc acatttatta tccgccctat acttatatcg tcatcgggat
     1621 ttaataccac tctcagatat gaagtgatgt ctttgatttc ccgcctgtga taaaacttaa
     1681 tacctcccac aatctgataa ggaactccga atctttgaca acccatttca aatgatctgg
     1741 attgggcgtt tacccgatac ataacagcga aatcagataa ggagtattct tcattatcgg
     1801 aaagtcgttg tatttctttt attacttttc gggcctcttc ttcttcgtca taggcttcat
     1861 caagtgatat caaactcccc ttatcgttac tagtccataa atttttttct actcgttgtt
     1921 tattctttgc gattacattt ttcgcggcac ttagaatatt ttgagaggaa cgatagtttt
     1981 gctccaaagg taggatttta gcttctggga aatcactttg gaaacttaat atgttcctaa
     2041 tgtctgcatt acgccaagag taaatcgatt gatcgggatc tcccacaacg cataagtttc
     2101 tggaagtttc agttatcatt ttggcaatcg aatactgggc tacatttgta tcttgaaatt
     2161 cgtctaccat gaaataatga aatcgggttt gatatatttc agccgcggta ggagattccg
     2221 ctaaaagttg atgggttttt aatagaagat catcgaaatc taaagcaact ccctggctca
     2281 atatttcttc atatctctca aaaatgcgac ccacgacctc ttcataatag ttggattttt
     2341 gggtattaaa accttggaaa ttcactagct gacttttgga gttggaaatg gtggacaata
     2401 cagatctcgg attaaactgt ttcgggtcta catccacatc cttcatactc ttttttatgg
     2461 cagctatttg atcatcatca tcataaataa cgaaattgtt tggaagtcct atatattctc
     2521 cacttctcct gagaatgacc gagcaaaagg aatggaaggt accgactgta agccttctgg
     2581 cttcatctcc tagcagtggt acgagcctat ctttcatttc cctagaagcc ttattggtga
     2641 aggtcacggc accaatattg taagggctca ctttttcatt gaggaccaaa tgtgcaatcc
     2701 tattagtgat tactcttgtc ttaccactgc ctggcccagc tactatcaga acaggtccgg
     2761 taactgactc aactgattct cgttgggctg gatttaattg ttccagaaaa tttttgttca
     2821 ttctttctct gctcagctac tggtaagtga aaataggatt tgattttgtt atcactctag
     2881 tctctgaaat aagaccttct ctggtgtatt tgggcaattg aaacatgcct ttatgtgata
     2941 agccatcata cattttcaga tcatgtattc cttgcctagc tagttcttca tcgatgaagg
     3001 cttcagtgtt ctcagcaggg tttattttgt cactagctgt gacaaacccc caagtagaac
     3061 caaatgaggg tacaaaaatc tcatatcctg cacagctact gaatgtttgt gaaatggtgt
     3121 ttgccacggc agtgaaacac tgctctgaga aagatggacc agtagcccct gcctgtatga
     3181 ccataatacc gttcatggtt agtttttgtt taaggagatc aaaaaactcc actgtaaaca
     3241 gttgaaaagc tgggccaaac tccaatgggt cagggacatc aataattgca acgtcaaacc
     3301 tattgtttaa cgactcaaca aattctaaag cgtctgaata atatagatcc aaacgagggt
     3361 tactgaaaga attcatatgg tgatttggta agaatttttt acatagttcc accaattttt
     3421 gatctatatc taccatggtc actttttcga cccctgggta ctttaaaacc tctctggcgg
     3481 tcgccccttc tccacctccc acaattagta cattttttgg atttgtgtgt gacaccatgc
     3541 tgggatgcac taatccttca tggtagacaa attcatcgag ttcagttgat tgggtcttgc
     3601 cgtccaatat gagagacttg ccgaaacatt ctccgtcaac aatcatcaca tgttgaaatt
     3661 cagtttgaat atcgcacagt acggagagga ttctttctcc ttgtatcaga ccggcgctaa
     3721 ttggctctat atgccattca ggtgacgata tgttcactta ccgtcacctg aatcttcttc
     3781 atcttctctt attcctcttt cgataattct agaaaaagaa tctcctgatt ctaatttggc
     3841 gctgatgatt ttgcaggctt tttccaaatc aaaatcttca ccacatgaaa aaatatctac
     3901 tgatgcatat aaatattctg gccaggtgtg gatacatata tgcgattcgg ctatcgaaac
     3961 gacgccggtt accccaacag gtttgaattt gtgaaatgtc tctccaatga tagtagcgcc
     4021 tgcagagttt gcagcttctt ttaagacatc ttttataaaa tcaatatcat caagaagacg
     4081 agaattgcag ttttctaaat ctaatagaat ttgggttccg agagacttca atattagtgc
     4141 accttggggt taggatgggg tccgaatcaa aaaatatgtt ggcttaggta gaattataca
     4201 ggaaccttaa acgataaatt tggccttgat tgacctacct ggccttgctc ccatatgcta
     4261 atctgaagtt atctattaga ggtgggaatt tgtcaactga aattaatctc agagttcttc
     4321 ttaaatcaag accgaaaggt ttcccaaccg aagatgattt tgatgttgtg gaatctgtga
     4381 ttccgcggcc acaggaagga gagattttac ttcaatcatt gtggttgtct ctcgacccct
     4441 acatgagggg gcgaatgaat gatgccaaat catacgcacc ttcagtaaat ataggtgacc
     4501 ctatggtagg gggggcagtt gctaaagttg tagagtctag atcgcctctg ttcaaagcgg
     4561 gagatatatt ggaagggagg tttggttggc agagttatgc cctgtcgaat ggattgggtg
     4621 tacggaaaat taatgaatcc ttggcaccct tgcagactgc agtaggtgtg ttaggtatgc
     4681 ctggattaac ggcctatttc ggctttttgg atgtttgcga acccacccca ggagatacgg
     4741 tggtagtttc tgcggcgtca ggcgcagtgg gtcaaattgt agggcaaata gccaaaataa
     4801 tgggctgtaa agtaatcggg acagctggaa ccgaatccaa agtttctttt atcgttgatg
     4861 aactaggctt tgatgcagga ataaattata aaaatactaa tgttggagaa gctttggacg
     4921 aattatgccc agaaggaatt gatgtatatt ttgacaatgt aggtggtatt gtcaccgatt
     4981 cggtgatgga ccgaatcaac acaggtgcaa ggattgctat atgtggtcaa atatcccaat
     5041 acaaccttga gtctcctgat atggccccta gaaacttagg tattttaact agatcgcagg
     5101 cgaaaatgca ggggttttta gtttttgcat atgaaagtag gtacgatgaa ggtatcacta
     5161 gaatggcctc ttggatcaag gatgggaaac tcaaatacag ggaggatgtt gtagtagggt
     5221 tgagaaatgc ccccagggcg tttattggaa tgctgaatgg agaaaatttt ggtaaaacct
     5281 tagtgaaagt ttcggagtaa cctatgcccg acagtctcac cccagccctg cttaaattcc
     5341 gagaggaatt ttccgaattc ctgttggaaa attcgggtag ttctgaagag gaatttaggg
     5401 agttaagctt ggatagaggg tactaccgaa tggctcagcc gatcgaattg ggtgggcgtg
     5461 cctcctctgc aatggaaatg ttaatcgctc gtgaaactat tgctaaatct ggcattttgt
     5521 atcctggagc tataatcggt ccggagcccg gacttttatc taaggcggac gggctcttga
     5581 aatccaacta tcttattcct ctcttacggg gcgagaaaaa aggagcattt gctttcacgg
     5641 agtcaaatgc tgatttacca actgttgctg aatgggatga tggccattta gtggtgtcag
     5701 gtgaaaaatc ctatgtttca ggaggacatt tgtctgattt catgtccgtt gtattaaccg
     5761 ttgatccgga aaaagtcacc gacaagtctg gcccggcagt gctagttatt gattcaaatt
     5821 ctaacggtat aaaaatggga gatccatttt atagtttaga tggcagttcc catgtggtgg
     5881 tggaattcca atcagtgaat gtagatgctg ccaatataat cggggaggta ggtcaaggaa
     5941 taccacgggc tttgggaaat ataatgcagg aaagaataga acaatctgct acgtcagtgg
     6001 gtttggccat gtatgccttg gatatagtga cggctcatct gaagaaaccc catcgatcag
     6061 gggggactct ttctgatttt gaaggagtga ggttgaggta tgccgacatg cgtatcaata
     6121 cctttgcggc aaggtctgtg ctgtacagag tagggagact cctaggttca gaggattcgg
     6181 ttgtaaatga agttacaaca gcgaagatat tttgtactga ggcggcttct gacgtagttg
     6241 atacagccgt ccaattagtc ggtggtcaag ccttagtttc tggacatccc ttggaagatt
     6301 tgtacagaaa aataagatcg atgcgacttg cgggtggtgc ttcagatatt ttgaggctta
     6361 atgtgagtaa gggagtattt gagttcgatt cagggattgt ctgaaaaaag ttctaactct
     6421 ggtaattaat caaatattac ttatcgagga ataactgttt tcttttgaga ttcagtagat
     6481 aatggaactg ccggtaatca ccaatttctg agatggatgc gataaaatct ccaacattgt
     6541 caaaatagcc tcgtaattcc cttaattctt tccctatagg gctaattgaa gcagggccct
     6601 cagcatagtt cccacgaaag gcaaaatatg cttggtttaa ttttctgatg ttgtaaccac
     6661 caagctttag tttccaaagc tgtttgtcca tatattgctc tgccttttcg atttccccat
     6721 tttcgagcat ttcttcgaca cccgttctgg tttcacgcaa tattttgacc agatgagtgt
     6781 tgcccgagtt caagttatcg tatcggtagg agttgacggt agtgttacca agggctttga
     6841 aggcttcgtc cccgagttcg ttacctgcta aatcggcagc agtttcattc aaaatctgca
     6901 tgttatgaga ggaccacatt tttctgccca gtggttttaa tatccaatag ttgtgtaacc
     6961 attcgtgagc tgctgtacgc attaattgcc tgagctcatc tttgtctgac acgatggctg
     7021 gataggttcc taggccagcg agatcgtcaa caagtgcgga agtgttttca tattgttccg
     7081 ccctttcttc aattcttgtc ctatcggacc actctaagtc agactttatc aatttacttc
     7141 ctgtaagttc tatcttgtct cttctggagg taataaggat atggggaggg tctccgagcc
     7201 tgaaatccgt gggcggaata agtaacccaa atggtagacc caagttatta ttacgtgcgg
     7261 cgctgctcac agaagattcg atggcctctt ctgccctacc tctgagtgct tctcttttcc
     7321 ttcgtaaata ttcaatttct ttctttttga gtgtcgtctg ggtcatcgat gttgtatcta
     7381 ccaagcggtt tagtgttgtt gaattgtcta agctaactag ctccttttgc agttttaatg
     7441 tcgtggctag gtaatcctta attagtgtgt ttcgttctgc gtcattaggc ttgtgcccag
     7501 ggtatttatc ccacaacaga tgtagccatt tcaaaggtaa atgtctgagt tcccaacgaa
     7561 ccaatgaaaa agcatgagat ccgacagcat agtcgctttg agtgaagtga tgaacatccg
     7621 atttggcaga tatgagaatc agaattgata ttatcgctgt gaatagcgtt cttttcccat
     7681 taactctcat gtttatatat ttcaatttgt gtgactttaa attaatgccc aggaaggtat
     7741 tgagctgtta gcaactgact ttaattgtct ctgtaatttt tgagacccag gtaatacttc
     7801 atttagtaaa taccaaaatt gttttgagtg gttcatctca tggatatggc ataactcatg
     7861 agttacaacg tacttaatta gactaggttc aaggaaaagt aaatttctat tgagatttat
     7921 atttttcttg tgagaacagc taccccaaat gcttttacgg ctgcctatct ttattttgtt
     7981 gtatgggatg tttaattttt tagaattggt ttctacagct ttttctaaaa ttgagacggc
     8041 tttaatctgt aaccattgct gtagaagttt tgagtttgtg attagagaca ctctttcctt
     8101 tggttcctta agaattatgg tttcatttgc ctctttgagt gaaaaatctt ctgtttccaa
     8161 tgctaaataa gtgacatcaa acaccttggt ggtggccgga agagaaatct ttgatggttt
     8221 gatgtcaaaa tttctatttt tgatatcatt caacttctga tttatatact gctcattctg
     8281 g
//
LOCUS       DASCNS010000284         3976 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002085588, whole genome shotgun sequence.
ACCESSION   DASCNS010000284 DASCNS010000000
VERSION     DASCNS010000284.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002085588"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /gene="modA"
                     /locus_tag="QF838_10000"
     CDS             complement(<1..499)
                     /gene="modA"
                     /locus_tag="QF838_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HJO61049.1"
                     /translation="MKKMRILTRWFIAAVALVLFLGCHSNEDELVVFAAASLTDVLTE
                     VKEEYEFQNKTIVQYNFGGSQSLAVELSKGSPGHIFISAGEPPMEFLYSEMGSRISGV
                     TSIATNSLVIVTNPQTAGLLDYSSLAELDLISLADPNLAPEGVYSREFLVNTGLWSFM
                     KDKFIF"
     gene            718..2301
                     /gene="nusA"
                     /locus_tag="QF838_10005"
     CDS             718..2301
                     /gene="nusA"
                     /locus_tag="QF838_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HJO61050.1"
                     /translation="MKSDFLIALTQLAAERNLPRDKVMSAIEAALVSAFKKDSVTEGH
                     NISVRLDPGSGDIEVDIVKTVVETVEDSLQELTLEEARANYNPNAGIGDVIKTQNIPN
                     SAGRIAAQTAKQVVLQRLREAEREIVLAEYEGREGEIITVTIQRIEPNKIIVDTGRTE
                     AILPVSQQVSSERYRQGSKLKVLLQAIDKESIRGPELIVSRADEILLRRLFEQEVPEI
                     YNGAVEIVGIARESGSRSKVAVRAKQDGVDPVGSCVGLRGVRIQSIVNELQGEKIDIL
                     EWSKDSNRYIQNALSPAEVLRVDVNNDNNTAVAIVPNRHLSLAIGKEGQNARLAAKLC
                     GWAVDIKSDMDVDLTAYGRQKEQSAPEIPEAPEATFTDEELYELVSSNLVTSNPASKQ
                     DSSITVESQEATVEKIETTHVGVEEIGEILQTESQDTEDKTEDALTPEEELLLLELEE
                     EEIQRTQPEETAAAISELPENIWSVHRSGAGSSQAGAIRFAEDIDELKSGVTGRRERR
                     GGGRSQNKKKGQGNNKRRR"
     gene            2476..>3976
                     /gene="infB"
                     /locus_tag="QF838_10010"
     CDS             2476..>3976
                     /gene="infB"
                     /locus_tag="QF838_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HJO61051.1"
                     /translation="MSSQIDQVENGEEAVAVIEIPAAIMVSDLADQLDVGAVEVVKAL
                     MRGGYMFSVNDVIEHEIASVVCQLFGFQATLLAESGAETNSIVIDNKDEDPKQLSTRP
                     PIVTILGHVDHGKTTLLDSIRKTNVVDGEAGGITQHIAAYQIKHNESPITFLDTPGHE
                     AFTAMRARGAQVTDIAILVVASDDGVMPQTIEAIDHARAADVPIIVAVTKKDLPNADL
                     DKVYRQLSERDLLIEEWGGEVISIPISGITGDGVPELLENIQLVAEISELKANPDRTA
                     QGVVVEARKDKSKGNIATLLVQTGTLSQGDILVIGNTYGKIRAMFDDQGDRIKSAHPS
                     TPVEVMGLHDIPEAGSIFKVAGNDKLAKEMVDIYERQVRAEATKLQDAHTRNVPGDLR
                     QVDLIIKTDVQGTIEPVKAALENLNSADSRVNVIHIASGGITERDVLLAVASDAVVIG
                     FRTLPEGGAGPLAKQEGVEIREYDVIYQLIEDVSKALEGLLQPTYGDVYV"
BASE COUNT         1341 a          760 c          944 g          931 t
ORIGIN      
        1 caaaaataaa cttgtctttc ataaaagacc agagaccggt attcaccaaa aattccctag
       61 agtagactcc ttcgggtgca agatttggat ctgctaaaga aatcaaatcc aactctgcca
      121 agctagaata atcgagcaaa ccggcagttt gtgggttagt tacaataacc aaagagttag
      181 tcgctatgct agtgactccc gaaatcctgg accccatttc actgtaaaga aactccatcg
      241 gtggttctcc agctgaaata aaaatgtgac ccggtgatcc tttggataat tccaccgcca
      301 aactttggga acccccaaaa ttatattgaa ctatagtctt attttgaaac tcatattctt
      361 cttttacttc agtcaaaaca tccgtgaggc tagctgcggc aaaaaccacc aattcatctt
      421 cgttggagtg gcagcctaaa aataacacca gggcaacagc cgcaataaac cacctggtta
      481 aaattctcat ttttttcata gtaataaagg aacttttacc cgtccttaaa tattttcttg
      541 ctctgttaat ttctacaaaa tattccttgg ttagacaacc tttaaccgta agtagtcaag
      601 gttgttccat taggcgtcac aatgctataa tgtttgcctg tttaaagagt agcaaattga
      661 aagtgggcga gtcccacttt ctttcgtatt agaggaaaaa aaaggaggca gtttaatatg
      721 aaaagtgatt ttcttatcgc tttaacgcaa ctagctgctg agagaaacct tcctcgagac
      781 aaagttatgt cagcaataga agcagctctt gtttctgctt tcaaaaaaga tagtgtaacc
      841 gaaggtcaca atatttctgt acggttggac cccggttcag gtgatattga agtcgatata
      901 gtcaaaactg tggtagaaac agttgaggac tcccttcaag aattgaccct tgaggaggct
      961 agagcaaatt acaatcctaa tgccggcatt ggagatgtaa taaaaactca aaatattcca
     1021 aatagcgctg gcaggatcgc cgctcagacg gcaaagcaag tcgtacttca aagacttcgg
     1081 gaggcagaga gagaaattgt cttagccgaa tacgaaggtc gagaagggga aattattact
     1141 gtaactatcc agaggataga acccaataaa atcattgtag acacaggaag aacagaagca
     1201 atattgccgg taagccagca agtttcatct gaaaggtacc gacaaggctc caagctgaaa
     1261 gtattactgc aagctatcga caaagagtca attagaggac cagaattgat agtttcgaga
     1321 gcagatgaaa tcctactgag gagattattt gaacaagaag tgccggagat ttacaacgga
     1381 gcagttgaaa tcgttggaat agcgagagaa agtggctcaa gaagtaaggt cgctgtcaga
     1441 gcaaaacaag atggtgtaga ccctgttggc tcttgtgtag gtctgcgagg ggtgagaatt
     1501 caaagtatag tcaacgaatt gcagggagaa aaaattgaca tactagaatg gagtaaggat
     1561 tccaatcgat acatccaaaa tgcattgagc cctgctgaag tgctccgagt agatgtaaac
     1621 aatgacaaca acactgcggt agctattgtg cctaatagac acctatcact cgccattgga
     1681 aaagaaggcc agaatgccag acttgcggcg aagctctgcg ggtgggctgt tgatataaaa
     1741 agtgatatgg atgttgatct tacagcgtat ggaaggcaaa aagaacaatc agcacctgaa
     1801 atccctgagg ctccagaagc aacattcaca gacgaagaac tatatgaact ggtgtcaagt
     1861 aatcttgtga caagtaaccc tgcctctaaa caagattctt caataaccgt tgaatctcaa
     1921 gaggcaactg ttgagaaaat agagacaact catgtgggag tggaagaaat tggtgagatt
     1981 ctacaaacag agtcccaaga cacggaagac aagactgaag atgccttgac tccagaggaa
     2041 gaattattac tgctggaatt ggaagaggaa gaaattcaga gaactcaacc agaagagact
     2101 gcagctgcca tatccgaact accagaaaac atatggtcag tccataggtc tggagctggg
     2161 tcatcacaag cgggggcaat cagatttgct gaagacatag acgaattaaa gagcggagtt
     2221 accgggcgaa gggaaagacg tgggggagga agaagtcaaa ataagaaaaa aggccagggg
     2281 aataataaaa gaaggcgtta attagccttg tgaaacttag ccatatcggc aaccagaaaa
     2341 attccataaa ttatttaata attggaaata gctccaagaa ggtaagtggt attgctatgt
     2401 gaatattggt aaccaggtag agttgccaaa atatcacagt taaagtgagt ctaaaatctg
     2461 ccctagagga aatgaatgag cagtcaaata gatcaagtcg aaaatggtga agaggcagtt
     2521 gcagttatag aaataccggc tgcgataatg gtcagtgact tagcggacca attggatgta
     2581 ggagcggtag aagttgtaaa ggccttgatg aggggcgggt acatgttctc tgtcaatgat
     2641 gtgatagagc atgaaattgc ctcggtagta tgccagttgt ttggattcca agccacttta
     2701 ttggcagaat ctggagcaga aaccaattct attgtcatcg acaacaagga tgaagatcct
     2761 aaacaacttt cgacacgccc accgatagtt accattttgg gacacgttga ccatggaaaa
     2821 accaccttgt tagacagcat cagaaaaact aacgttgttg atggagaagc aggaggaatt
     2881 acccagcaca tagccgctta ccaaataaaa cacaatgaat ccccgataac gtttttagac
     2941 actcccggcc atgaggcctt cacagcaatg agagcaagag gggcccaggt aacagatatt
     3001 gcaatactgg tagtagcctc agatgatggc gtgatgccac aaactattga ggctatagat
     3061 catgccaggg cggcagatgt accaataatt gtagctgtga caaaaaaaga tctccctaac
     3121 gcagatcttg ataaagtgta cagacagtta tcagaaagag acctgcttat tgaagagtgg
     3181 ggaggagagg taatctcaat cccaatctca ggtataaccg gtgatggagt tccagaattg
     3241 ctggaaaata tccagctggt tgctgaaatt agcgaattaa aagccaatcc ggatagaact
     3301 gcacaaggtg tggttgtaga agcccgaaaa gataaaagca aaggaaatat agcgacgcta
     3361 ttggttcaaa ctggaacact tagccaaggc gacatattgg tcattgggaa cacatacgga
     3421 aagattagag ccatgtttga tgatcaagga gatcgcatta aatcagccca cccatccaca
     3481 cctgtggagg tgatgggact tcatgacatt ccagaagcag gctcgatttt taaggtagct
     3541 gggaatgata agctcgctaa agaaatggtt gatatatacg agaggcaagt tagagcagag
     3601 gcaaccaaac tccaagacgc ccacaccaga aatgtaccag gtgatctaag gcaggttgac
     3661 cttattataa agacagatgt acaaggtact attgaaccag ttaaagcagc gctagaaaat
     3721 ctcaacagcg ccgactctcg agtgaatgtc attcacatcg caagcggtgg cattactgaa
     3781 cgagatgttc ttctggcggt ggcatcagac gcggtggtaa ttggatttag gactctcccg
     3841 gaaggtggtg ccgggccttt agcaaaacaa gaaggtgtgg aaataaggga atatgatgtc
     3901 atttaccagc tcatagaaga tgttagtaaa gcattggaag gattgctcca acccacctat
     3961 ggagacgtat acgttg
//
LOCUS       DASCNS010000285         8565 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002086458, whole genome shotgun sequence.
ACCESSION   DASCNS010000285 DASCNS010000000
VERSION     DASCNS010000285.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8565)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8565
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002086458"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(92..1507)
                     /locus_tag="QF838_10015"
     CDS             complement(92..1507)
                     /locus_tag="QF838_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61052.1"
                     /translation="MNSYQSKTIALCLILSETVWIYSIFAVVGIMTSLGRSPLSYGGC
                     LALYAVSLYLSKLLNSLNIKVSLAICFQTLVGTILCYFIVGYANLPSQETISIYWIFN
                     LDHWVYEDGEVGLAIVLAAFMSVFMWTKGGLTGSTDFPLEALFATFRIGVIVMAFTVT
                     IDIFSGADLYMSRLMYLFFVSTLAGLAIGRLKPSSDSGAVTLQWLRVVSILVGMIVLS
                     GLLFASFSKDFLGAISSPITTLISWLTMAIVYLLAIPIIYAIQFVIRFFSWMFGDTLT
                     PDRPVQEQESFGGLSNVLEELVNAPGSEEPSAFMQYFEYFAIFLLVAGLLFGLGLAFR
                     RINKSKNIVDEGIKSTIEGSGEPMTDLLDIAKRLLWKRGLSKQETMFVIPSHLDPGSK
                     SVLESYYDLLYLGYQKGVNRSDNSTPKEFEPALYRFFGIDPVRKISEAFMDVCYGRIT
                     PVSSRVAEIKELIDGLDKEHH"
     gene            complement(1488..2621)
                     /locus_tag="QF838_10020"
     CDS             complement(1488..2621)
                     /locus_tag="QF838_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013998.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HJO61053.1"
                     /translation="MSLVVVFVAWLWNKVSLENVEYIRVIPQKRVFIGETVPFSVELH
                     NKKPIPLPRIDTVDDIPSALNLNGPDIRPSSTVDAVSMTHSASISAYQRTAWEYSVQA
                     KKRGFHRLGSVNIYGGDIFGLFDSKKTIATRDYILVYPQILKLPDLGMPESRPLGEKI
                     TNLTIYQDISWNRGIRTYEKGDSLNTVDWKYTAKKSELMVKTFESTSKSDVILAVSVE
                     TSSKVWEGYSPVNLERVVAASASIANYCSEEGFNLGFFSNGTPVLSDLPMRIPASQSE
                     DQLKVILETLATIGPISSGPMSENLNKWYRNFPFGSTVVVVTSFITDQLLASLRNISK
                     FGCRVVLINVANEPSYSAADNILIYELGTHFSEMESKGEFVPI"
     gene            complement(2733..3689)
                     /locus_tag="QF838_10025"
     CDS             complement(2733..3689)
                     /locus_tag="QF838_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HJO61054.1"
                     /translation="MVTEGSEFKGIIEKIKNNVQKVIVGKSDAIALVLISILCQGHIL
                     LEDVPGSGKTTLARAVSSSLDCTFGRIQFTPDLMPSDVLGVNWFNQKSSEFEFQSGPI
                     FNQIILADEINRATPRTQSALLEAMQESQVTIDGETRMLPTPFIVMATQNPLDMEGTF
                     PLPEAQLDRFLMKISLGYPTVQEEADIIRRFRKEDSHEQLASVVNSKVIKELQNFSSG
                     IKVEQTICDYIISIVRSTRENDKLDLGSSPRGSLGLYRCSQAKAALNGREYVTPDDVK
                     SLINAVLSHRIIPTSNARLRGFGADEILEEIMSETTVPMEAS"
     gene            3961..4034
                     /locus_tag="QF838_10030"
     tRNA            3961..4034
                     /locus_tag="QF838_10030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3993..3995,aa:Gln,seq:ttg)
     gene            complement(4039..4773)
                     /locus_tag="QF838_10035"
     CDS             complement(4039..4773)
                     /locus_tag="QF838_10035"
                     /inference="COORDINATES: protein motif:HMM:NF027439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJO61055.1"
                     /translation="MVIPMKDETIMYHSINGVAHITLNRPDQINAFNLEMRDALFQTL
                     ELFRDDRESKVAIISGAGERGFCAGADLTEFGTAPSQVIARAVRWERDLWGLFLTIQK
                     PLIAKLHGYVIGSGIEIAALCDIRIAADNSIFRMPEVALGMIPAAGGTQTLRNLVGLD
                     RAMEMIMTNRVVKAKEAIKVCLVDRVVSEETLDSSAEVIAGGLAKIEPELLASIKSSV
                     MRGLDFGLERGIQHEKRNSYHTAKKH"
     gene            complement(4786..5517)
                     /locus_tag="QF838_10040"
     CDS             complement(4786..5517)
                     /locus_tag="QF838_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJO61056.1"
                     /translation="MVNLQSFNEEKIQLINLKSTDKNKDPCRELLWLLEDAINELNAK
                     DSVAVVLINIPDLKLRCDWHAQNDGTKSLSGFISSIKFPTIGIVESEAHGTALELFLC
                     CDIRVADKVSKFSMSQVLAGHTPVEGGTQRLPRIVGVGRSMELLLTGRVFSAAEALEI
                     GVVQYLYSKNVQDNAVELASKISSHGPIAAKYLKEAIVDGSDMTLDQGLRLETDLNVI
                     LQSTFDRKEGIGSFLEKKEPRYEGR"
     gene            complement(5555..6685)
                     /locus_tag="QF838_10045"
     CDS             complement(5555..6685)
                     /locus_tag="QF838_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HJO61057.1"
                     /translation="MQQPGNLNASLEKVAKVSFYVFIGVVVCFGLYLVRGALFPLVLG
                     IVLTYILNPFVNFFEQFIPLKSTKPKLARALSITIVIVTAFLIITGSLLLVVPPMFKQ
                     SSELITNIPSLITDARTTVESWNKEYASNIPEEVRVEIDRLLENGGEILLGAFSGLIG
                     RTAVAAVHALTLVIGLVVLPLFVFYFLKDRETVRESFVEIFPTDTQIHVVYVLRILNR
                     IIGAYVRAQFTLAAVVWVFISIGLMLMGVKYAILLGAVAGIFELVPIVGAWLAIIPTI
                     IVVLSTSPEKILWVLLLYLGVQLFQGAIIVPRVQSFAMKLHPLMILISIVVGSEIAGL
                     WGVVLGPPIAASIKELLIYFSNPPRYGISMEDDEVLEKELDN"
     gene            complement(6817..7452)
                     /gene="jag"
                     /locus_tag="QF838_10050"
     CDS             complement(6817..7452)
                     /gene="jag"
                     /locus_tag="QF838_10050"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HJO61058.1"
                     /translation="MKSIEMTAKTVEEAIEIALKELDVDRVEVEIDVISRGKPGILGI
                     GSELASVRLTKVDGTSDVLDSANEVIKNLVSHMDVDVVVNLKEADNEETGGPVFEIEG
                     DDSGLLIGRKGETLRSLQFLTRFIVGRQTGERANISLDVEGYDERRKQSLANLANRVA
                     QRVVKTGRSIELEPMSARERRLVHVALADNSEVYTESSGEGRDRRVVVIPS"
     gene            complement(7349..8221)
                     /locus_tag="QF838_10055"
     CDS             complement(7349..8221)
                     /locus_tag="QF838_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HJO61059.1"
                     /translation="MINSLLILYSIAISNFGLAIILFTVLIRATMMPLTVKQSKQMKS
                     MTALQPKIKEIQNKFKNDKQKQSQETMKLYKEQGVNPIGCLGPMFIQFPIWIGLYQAI
                     LQTVPNTPESLVNLSGHLYEWLPLVHDVVPINSNFLWLNLANPDPFPIFPILVGVSMW
                     FMQKMTTMPAMDDRQASTNKMMLWMMPIMFGFFTMQFPSGLALYWVVSNVVGIVIQGF
                     ITGWDPIKNLLSFASNRTPQPAAAVAGGLSPVLEEEQTDEVDRNDSEDSGRGDRNRPK
                     RTRRRPRRGRNRRH"
     gene            complement(8288..>8565)
                     /locus_tag="QF838_10060"
     CDS             complement(8288..>8565)
                     /locus_tag="QF838_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61060.1"
                     /translation="DMIAVGSTDGEIRIAETSSGEVLASCDVGESIESPITADGFEIY
                     FSVRDHSVRALTVKANGNPDEKWDAPYFSDLLKDDKNPQPGDWSPDC"
BASE COUNT         2581 a         1993 c         1518 g         2473 t
ORIGIN      
        1 attatggtaa agctgcaaca tacttaactt ttagtgtagt cgccgggtta ttggcagctt
       61 tcctgggcat atggattgca aaacaaacga tttagtgatg ttccttgtct aagccatcta
      121 tcaactcttt gatctcagct actcgagatg aaaccggagt gattctccca taacaaacgt
      181 ccatgaaagc ctccgatatt tttcgaacag gatcaattcc aaaaaaccta tataaggctg
      241 gctcaaactc ttttggagta gaattatcgg atctattcac gcctttttga taacctaaat
      301 acaacaggtc ataataagat tccaacacgc tcttacttcc aggatccaaa tgacttggta
      361 tgacaaacat ggtttcctgc ttggataatc ctcgtttcca gagcaacctc ttagcaatgt
      421 caagcaaatc agtcatgggc tctccagacc cctcaatcgt agatttaata ccctcatcga
      481 ctatgttttt tgacttattg atccttctga aagcaaggcc taggccaaat aacaagccgg
      541 ctaccaagag gaagattgca aaatattcaa aatattgcat gaatgctgaa ggttcttctg
      601 atccgggtgc attaaccaat tcttctaata cattactcag gccaccaaat gattcttgtt
      661 cttgaactgg tcgatcagga gtgagagtgt caccaaacat ccaactaaaa aatcgaatta
      721 caaactgaat tgcataaata ataggtatcg caagaagata aactatcgcc atcgtaagcc
      781 aacttatcag tgtcgttatg ggggaggaaa tagcaccaag aaaatcttta ctgaaggagg
      841 caaaaagtaa gcctgataaa acgatcatac ccaccagtat cgacaccact ctgagccatt
      901 gtagagtgac ggctccacta tcagaagaag gttttaagcg tcctatggcg agacccgcca
      961 aagttgagac gaaaaacaga tacattagtc tcgacatgta caaatccgcc ccgctgaaaa
     1021 tatcaattgt gacggtaaaa gccatcacta taaccccaat tctaaaggtc gcaaataatg
     1081 cttctaaagg gaaatcagtt gaaccggtga gcccgccttt tgtccacata aacacactca
     1141 tgaaagcagc caaaactata gctaacccaa cttctccatc ctcataaacc caatgatcca
     1201 agttaaaaat ccagtatatt gaaatggtct cctgtgaagg taaattggca tacccaacaa
     1261 tgaaataaca gagaatcgtt ccaaccaacg tttgaaagca aatggcaagt gagactttta
     1321 tatttaaaga attaagtagc ttggataaat atagggatac agcatataaa gcaagacatc
     1381 ctccgtacga tagaggactc cgcccaaggg aagtcattat tccaacaaca gcaaaaatcg
     1441 aataaatcca cactgtttca gatagtatta agcacagtgc aatagtttta gattggtacg
     1501 aattcacctt ttgactccat ctcactaaaa tgagttccca gctcatatat caaaatattg
     1561 tctgctgcac tatatgacgg ctcgttagca acattaatta aaactactct gcatccaaat
     1621 tttgatatat ttcttaaaga agccaacagc tgatcggtga tgaaggatgt aacgaccact
     1681 accgtagacc caaaagggaa atttctatac cacttattaa gattttctga cataggccct
     1741 gatgaaatag gacctatggt agccaaggtt tctaatatta cctttagctg atcttctgat
     1801 tgtgaggccg gtattctcat tggcaggtca gacaatacag gggtgccgtt tgaaaaaaaa
     1861 ccaaggttga acccctcctc actacagtag tttgctatgg aagcagaagc agccacaacc
     1921 ctctctaaat ttacaggaga gtagccttcc catacctttg aagacgtttc tacagaaaca
     1981 gccaaaatca cgtcactttt actggtagat tcaaaggttt taaccatcaa ctcagatttt
     2041 tttgctgtat atttccaatc gactgtattg agcgaatctc ccttttcata agttctgatt
     2101 cctctattcc aagagatatc ttggtaaata gtaaggttag tgattttctc tcctaaaggc
     2161 ctagattcag gcatcccgag gtctggaagc tttaaaattt gcggataaac cagtatgtag
     2221 tcgcgagtcg caattgtttt tttactatcg aacaatccaa aaatatcgcc accataaata
     2281 tttactgagc caagcctatg aaaacctctt ttttttgcct gaacagaata ttcccaagca
     2341 gttctttgat aagccgatat agaagccgaa tgcgtcatag acacggcgtc aacagttgaa
     2401 cttggtctga tatcaggtcc attaagattc aaagcgcttg gaatgtcatc aacagtatct
     2461 attcttggta aaggtatcgg ttttttattg tgaagctcta cagagaaggg aacggtctct
     2521 ccaataaaaa ctctcttttg gggtatcaca cgaatatatt cgacattttc cagcgatacc
     2581 ttattccata accatgcaac aaaaacaaca actaagctca tgacagataa tcctaggatt
     2641 aaaccttgcc ccgaaaatag gccgataatt accaaggcaa taattattaa tacccaacag
     2701 tccttccaaa atttttttac catgatttat gcttagctgg cttccattgg gacagtagtt
     2761 tcagacatta tttcttcaag tatttcatct gccccgaatc ccctcaatct agcgttagat
     2821 gtcggtataa tccgatgcga caaaactgca tttataaggc tttttacatc atctggggta
     2881 acatattccc ttccattaag ggccgcttta gcctggctac aacggtaaag gcccaaagaa
     2941 cctctaggac ttgaccctaa atccaatttg tcattttctc gcgttgaacg cacaatcgaa
     3001 ataatatagt cacagatagt ctgttcgact tttattcctg aagaaaagtt ttggagctct
     3061 ttaatcacct tagaattcac cactgaagct agctgctcgt gagaatcttc tttccgaaag
     3121 cgccgtataa tgtctgcttc ttcctgcact gtgggatatc caagagatat tttcatcaaa
     3181 aatctgtcta gttgagcctc aggaagggga aaggtgcctt ccatatctag aggattttgc
     3241 gtagccatca caataaaagg ggttggtaac atacgagtct cgccgtctat cgtaacctgt
     3301 gattcttgca tcgcttctaa aagagctgac tgtgtccggg gagtggctct gttaatttca
     3361 tcagctaata taatttggtt gaagataggg ccgctttgaa attcgaattc actcgatttt
     3421 tggttgaacc aattaacccc taggacatcg gatggcatta agtcaggagt aaattgaatc
     3481 cttccaaaag tgcaatccaa agacgacgat actgcccttg caagggtggt tttacctgag
     3541 ccgggcacat cctcaagaag gatgtgcccc tggcaaagta tagaaattaa gaccagggct
     3601 atagcgtctg atttaccaac aataactttt tgaacattgt tctttatttt ctcaatgata
     3661 cctttaaatt cgctaccttc agttaccaaa aaagcatccc ccttccatta aaaattttca
     3721 ataagaggca atcatacaat caattgaatc aaaaataccg actaattctt taatttcaaa
     3781 agaaattatt ttgtccaact tactaaaagg tattaagaaa accatgataa atctaggagt
     3841 tctagagcgg ttggggaaaa gcctactgaa accagagtgt agacatgtac gagggaaatt
     3901 tttacttgag ccatatttat tgggatatac ttaatctttg tgagtctctt aatgtgtcgt
     3961 tgcccggtgg tgtagtggta gcactaagga ctttgactcc ttcgacccag gttcgaatcc
     4021 tggccgggca gccatggatt aatgtttttt tgcggtgtga tacgagttac gtttctcatg
     4081 ttggattccg cgctctagcc caaaatccaa tcctctcata acagatgatt ttatcgaggc
     4141 caacaattct ggctctattt tagctaatcc cccagcaatt acttctgcag agctatccag
     4201 agtctcctct gaaacaactc gatctacaag acaaaccttt attgcttcct ttgctttcac
     4261 taccctattg gtcattatca tttccattgc tctatcaagt ccaacaagat tacgtaaagt
     4321 ttgagtccca ccagcagcag gaatcatgcc caatgccact tctggcattc tgaaaattga
     4381 gttatccgct gctatcctaa tatcgcataa ggccgcaatt tcaatgccag acccgataac
     4441 gtatccatga agcttggcaa ttagcggctt ttgaatagtc aggaataagc cccacaagtc
     4501 tctctcccac ctcactgctc gcgcaattac ttgagatgga gcagtcccaa attctgtcaa
     4561 atcggcacca gcgcaaaacc ctctctcgcc tgccccactg ataatcgcaa ctttggattc
     4621 cctatcatcc cgaaataatt ctagtgtttg aaaaagagca tcccgcattt ctagattaaa
     4681 ggcatttatt tgatctggtc tgttcaaggt tatgtgagcg acaccattga tagagtggta
     4741 cataattgtc tcatctttca ttggtatcac cattttgatt caccttcagc ggccttcata
     4801 cctaggttct tttttctcca aaaaagaacc tatcccttct tttctatcaa aggtgctttg
     4861 caaaatcaca ttaagatccg tctccagtct taatccttga tccaaggtca tatcagaccc
     4921 atcaactatt gcctctttca gatattttgc cgctatcgga ccatgagagg atattttgga
     4981 ggccagctcc acggcattat cttgtacatt tttcgagtac aggtattgca ctactccaat
     5041 ttcgagtgcc tccgcagcag agaaaactct ccctgtcaat agtaattcca ttgaccgacc
     5101 tacgcctact atccttggca gtctttgcgt cccgccttcc acaggagtat gtcctgctaa
     5161 cacctgagac attgaaaatt tactcacttt atcggcgact ctaatgtcac agcataaaaa
     5221 taactcaaga gctgtcccat gagcttcgga ttcaacgatt ccaatggttg gaaattttat
     5281 gctagatata aaaccactca aagatttggt tccgtcattt tgggcgtgcc aatcacacct
     5341 caatttaagg tcaggtatat tgatcaagac cacagccaca gagtctttgg catttagttc
     5401 atttatggca tcttccaaca accacaggag ttcacgacac gggtctttgt tcttatctgt
     5461 cgattttaga ttgataagtt gtattttttc ttcattaaaa gattgtagat tcaccatatt
     5521 actcgactat gaaagttctt cattaaaaat aaaatcaatt atctaattcc ttctctaaaa
     5581 cctcatcgtc ttccatcgaa ataccatacc ttggcggatt gctaaaatat attaataatt
     5641 cctttatgga agcggcaatc ggtggaccca ataccacccc ccaaagacct gctatttcac
     5701 taccaaccac gatagaaatc aatatcatca agggatgcaa tttcatcgca aaactttgaa
     5761 ctcggggaac tataatcgct ccctgaaaaa gttgcacacc taaatataaa agcaacaccc
     5821 aaaggatttt ttctggggaa gtggatagaa ccacaattat agtagggata atagcgagcc
     5881 aggctcctac aatcggaact aactcaaata tcccggccac agctcccaaa agaattgcat
     5941 acttcacccc cataagcatc aatccgattg aaataaagac ccataccacg gctgctaaag
     6001 tgaattgagc cctaacatat gcaccgataa ttcgatttaa aattcttaac acatacacaa
     6061 catgtatctg ggtatctgta ggaaatatct caacgaacga ctctcgaact gtttccctat
     6121 cttttaaaaa ataaaagaca aataaaggca acaccacaag ccctatcacc aaagtcaggg
     6181 catgaaccgc tgcaactgca gttctaccaa taagtccact aaacgctcct agtaatatct
     6241 ctcccccatt ttccaataat ctatcaattt ccacccggac ctcttccggg atattcgaag
     6301 catattcttt attccaactc tcgaccgtgg ttcttgcatc ggtaataagg ctaggtatgt
     6361 tggtaattaa ctcacttgat tgcttaaaca tcggaggtac cactagcaat aagcttcctg
     6421 tgattattaa aaatgcagta acaatgacta tggttatcga caaggctcga gctagtttag
     6481 gtttggtact ttttaatggg ataaactgtt cgaaaaaatt aacaaaaggg ttaagtatat
     6541 aagttaaaac tattcccaat accaagggga atagggctcc cctgacaaga tataatccaa
     6601 agcaaacaac caccccaata aaaacataaa atgacacctt ggccactttt tccagtgagg
     6661 cattaagatt gccgggctgc tgcatagtat cgaacccaaa tattgactgt ctgacccatt
     6721 atgttactag aaagttgcac aagcgaggga gcacatgttc aaaaagctca aggctgtcta
     6781 tctaggagta aatctagagt ccttaatttc tgagcattat gacggtataa ctaccacccg
     6841 cctatcacgc ccttcacctg agctctcagt atacacttct gaattgtcag ctagcgcaac
     6901 gtgaactaat ctcctctctc tcgctgacat aggctctaat tcaattgatc tgccggtctt
     6961 tacaactcgt tgagccactc tgttcgctag gttagccaaa gattgctttc gtctttcatc
     7021 ataaccttca acatcaagag aaatatttgc cctttccccc gtttgccttc caacgatgaa
     7081 cctggtcagg aattgaagag acctaagggt ctcacctttg cggccaataa gtagcccgga
     7141 atcgtcccct tctatctcaa agacaggtcc tccagtctct tcattatcag cctcttttaa
     7201 attgaccacg acatccacat ccatatgaga aacaaggttc tttataactt cattagcgga
     7261 atctaaaaca tctgaagttc catcaacttt ggtaagtcta acagaagcta attcactgcc
     7321 tattcctaat atacccggtt tacctctgct aatgacgtct atttctacct ctacgcggtc
     7381 tacgtctagt tcttttaggg cgatttctat cgcctcttcc actgtcttcg ctgtcatttc
     7441 tatcgacttc atctgtttgt tcctcctcca gaaccggtga aagcccacct gcaacagctg
     7501 cagctggctg aggtgtccgg ttgctggcaa aactaagtaa attcttaatc gggtcccaac
     7561 ccgtgatgaa cccttgtatt acgatcccga ccacatttga cactacccaa tatagagcga
     7621 gaccgcttgg gaattgcatg gtgaaaaatc caaacattat gggcatcatc cacagcatca
     7681 ttttattggt agaagcctgt ctatcgtcca ttgccggcat tgtggtcatc ttttgcataa
     7741 accacatgga aacaccaacc agtattggga aaataggaaa agggtcaggg ttagccagat
     7801 tcaaccaaag aaaattgcta tttataggaa ccacatcatg aaccaatggt aaccattcat
     7861 acaaatgacc agacaagtta accaagcttt caggggtgtt cggcactgtc tgtaatattg
     7921 cttgatatag gccaatccaa ataggaaatt gtataaacat tggtcccagg caacctatag
     7981 ggttaacccc ctgttccttg tacagcttca tcgtttcctg agactgtttt tgcttatcat
     8041 ttttaaattt attttgtatt tcttttatct tcggttgcag ggctgtcatc gacttcatct
     8101 gcttactttg ttttaccgtc aaaggcatca tagtggcgcg aataagtaca gtgaaaagta
     8161 ttattgccaa accaaagttg ctgattgcta tgctataaag gattagcaaa ctatttatca
     8221 tcggctggat gatcacgcta ttccataatt gagaaaaaat ttccaagtct attgtcctct
     8281 atttttatta gcaatccgga gaccagtcac ccggctgtgg attcttatcg tctttcaaca
     8341 aatcagaaaa atatggtgca tcccattttt catcgggatt cccatttgcc ttcacagtca
     8401 aggctctaac tgaatgatct cgcacgctga aataaatctc aaatccatct gctgtgatcg
     8461 gggattctat actctctccc acatcacagc tagccaacac ttctcctgaa ctcgtttcgg
     8521 ctatccgaat ctccccatca gtggaaccca cggctatcat gtctg
//
LOCUS       DASCNS010000286         1106 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002086747, whole genome shotgun sequence.
ACCESSION   DASCNS010000286 DASCNS010000000
VERSION     DASCNS010000286.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002086747"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1106
                     /locus_tag="QF838_10065"
     CDS             <1..>1106
                     /locus_tag="QF838_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014774.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HJO61061.1"
                     /translation="ALMKAVNEGRLEISNQVMRHWDLCVQCRACEDVCPSGVPYGNLI
                     EATMDHVRDYRRIGLLPRLVYEIALRYLLPNQKNLSVLVSMMDFYIKSGFQKLVRKTK
                     ILKLISRRLENLEMMTPTLNGEFFRADGHLYQSGMADAEKIQMLTGCVMPLTQGNQMR
                     AAVRVLNKAGKDVEVPSSQVCCGAINSHVGDVEKTKDLARENIEAFSNGNPDGVIVVA
                     SAGCGARMKEYGPLLADDPEYSSKATNFSKRVVDINEYLNGTELQLGESVVPRVVTYQ
                     DSCHLANVQKVRDAPRDLLKSIEGVDFCELEEANICCGAGGTYMITEPEMAKGILANK
                     MKNIIASKADTIATANPGCFMQLQNGIREAGLGV"
BASE COUNT          337 a          186 c          289 g          294 t
ORIGIN      
        1 tgctttgatg aaggcagtta atgaagggag attagaaatt tctaatcaag tgatgaggca
       61 ttgggactta tgcgttcagt gtcgggcctg tgaggatgta tgtccttcag gagttccata
      121 cgggaattta attgaagcca ccatggacca cgtgagggat tatcggagaa ttggattgct
      181 tccgcgcctg gtctatgaga tcgccctgag gtatttatta ccgaatcaga aaaacctttc
      241 ggttttagtt tcaatgatgg atttttacat caaaagcgga ttccaaaaat tggtgaggaa
      301 gacaaaaata ttgaaattaa tctcaagaag attagagaat ctggaaatga tgacaccgac
      361 tttaaacggg gaattttttc gggccgacgg ccatctgtat caatctggta tggcagatgc
      421 tgagaagatt caaatgctta caggttgcgt catgccactc actcagggta atcaaatgcg
      481 ggcagcagtc agagttttga ataaagccgg gaaagatgtt gaagttcctt cgtcccaagt
      541 ttgttgtgga gcaatcaatt ctcacgttgg ggacgtagaa aaaaccaagg acctagctag
      601 agaaaatata gaagcttttt ctaacggaaa tccagacgga gttatcgtgg tggcttcggc
      661 aggatgtggg gccaggatga aggaatacgg tcctctgctg gctgacgacc cagaatatag
      721 ttcaaaggct actaacttta gtaaaagggt tgtcgacata aatgaatacc taaatgggac
      781 tgaattacag cttggtgaat ctgttgttcc tcgggtggtt acctatcaag actcgtgcca
      841 tcttgcaaac gtacaaaaag taagagatgc acctagggat ttactaaaat ctatcgaagg
      901 agtagatttt tgtgaattgg aagaagccaa tatttgttgt ggggcgggtg gaacatatat
      961 gatcactgag cctgaaatgg caaagggaat tttggccaat aaaatgaaaa atattattgc
     1021 gtccaaagct gacacaattg caacagcaaa cccgggttgc ttcatgcaat tacagaatgg
     1081 gattagagag gctggattgg gcgtgg
//
LOCUS       DASCNS010000287         4294 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002091535, whole genome shotgun sequence.
ACCESSION   DASCNS010000287 DASCNS010000000
VERSION     DASCNS010000287.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002091535"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="QF838_10070"
     CDS             complement(<1..582)
                     /locus_tag="QF838_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61062.1"
                     /translation="MERPTSKPTGHHHLELDTPALLVNSKLLKSNIENAKSYLKDTSC
                     KLIPNIGTHGAAGIARYQTNFDHEAPVFVDTLQQAHSFANNGFKNIIIESPPVGTGKV
                     ESLHRLVNTAHVTISISNLAHLNMLKHFSSENLSALFRIPNKSDSIGVGTHQTLLELV
                     KLLEEHFEINWKGLITVSKGSDSLEESFKEILSA"
     gene            641..1114
                     /locus_tag="QF838_10075"
     CDS             641..1114
                     /locus_tag="QF838_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61063.1"
                     /translation="MEPFTKQTQGTHQIYPSDKGLSYTITGKDGKREGFIKYDSVESV
                     YVESISRQSRFTFVWLAMLVVLIFIAMAALDGFLMKMIALSVFTLMGSFLLFDHYSQE
                     IQPCLVIQSNGFSAKFPLEKDLADYELRDLMGTVLEYIDTHNSINTSTIRVFIPR"
     gene            complement(1101..2138)
                     /gene="tsaD"
                     /locus_tag="QF838_10080"
     CDS             complement(1101..2138)
                     /gene="tsaD"
                     /locus_tag="QF838_10080"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HJO61064.1"
                     /translation="MNVLGIETSCDDTAAAVITDGTYIRSNIVASQSDMHKKYGGIIP
                     EIASREHFTSLIPVVGEALNEADMTHKDIDAIAVTYGPGLAGSLLIGVNSAKGLSMSW
                     DKPLIGINHLEGHIYSAWLEDLMPDSKAEFPIVCLIASGGHTDLILMKDHLEYELLGK
                     TRDDAAGEAFDKAARVLGLGFPGGPEIQKMAEKAKDEMDPLPRPQIKNSLDFSFSGLK
                     TALVRRAESEGFYPLVDNRGPSAYQTTEYSFAFQEAIVDCLIRNTKQAVLETNSKAVI
                     LGGGVAANSRLRQRMNEVIDIPIFVPRAPLCTDNGAMIGAAAFFNLKNGLPFQLDMDA
                     VPNLRIGLTAE"
     gene            complement(2135..2797)
                     /gene="rpe"
                     /locus_tag="QF838_10085"
     CDS             complement(2135..2797)
                     /gene="rpe"
                     /locus_tag="QF838_10085"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HJO61065.1"
                     /translation="MPEFKLYPSILSADFSQLGKQIQETETCGADGIHIDVMDGHFVP
                     SITFGTPIIKAVRDLTGLPLDIHMMTVRPENHFSEFASAGADSITVHYEASPELKDTL
                     ALLDKFDVRKCVALNPETPIEKIYDIIPLVDQILIMSVQPGAGGQDFIPESLKKIETL
                     SKEIESQGLSIPIQVDGGINPDTTLKAVQSGANIVVAGSAVFNEDFPICDGISNLRNS
                     SI"
     gene            complement(2790..4175)
                     /gene="argH"
                     /locus_tag="QF838_10090"
     CDS             complement(2790..4175)
                     /gene="argH"
                     /locus_tag="QF838_10090"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HJO61066.1"
                     /translation="MSNNKNQNPVIKGLSDKVGDFTVSLHYDRRLFKQDIKGSIVHAS
                     MLCKQKIITLSDKDSIVEALLEIESEIETGNFLWRPELEDIHMNIEARLHEKIGSVAG
                     KLHTARSRNDQVSLDMRMYVKDSINIIIRQLTTLQRILLDIASQNNDVIIPGYTHLQK
                     AQPVLFSHHLLAYFEMLTRDKTRFKEAYERTDVLPLGSGALAGLPYDLDRSYVARELG
                     FSEISQNSMDAVSDRDFVIDYLSAASICMMHISRFSEELILWTTEEFSLIKLPAEYTT
                     GSSIMPQKRNPDFAEISRGKTGRVYGNLVSILTVLKGLPMTYNRDLQEDKEGFFDSHD
                     TLAAVLEVFLGMLPGIQVDSVKARQAAEGGMVLATDLADYLVIKGVPFREAHGIISEL
                     SDYASTNHKLFSEISLEEYRGFSMLFDEDVYEVSVESSVNARNTVGGTSFKMVQQAIK
                     LGYQKLEKQDA"
BASE COUNT         1234 a          897 c          840 g         1323 t
ORIGIN      
        1 ggcagataga atttccttaa aagattcttc taagctgtca ctacctttag agacggtgat
       61 cagtcccttc caattaattt cgaaatgctc ctcaagtagt tttacaagct ctaataatgt
      121 ctgatgagtt cctactccaa ttgagtctga tttattcggg attctgaaca gggcggagag
      181 gttttcgctg gagaaatgtt taagcatatt tagatgagcc aaattggaaa tagatatggt
      241 gacatgagct gtgttcacca gtcggtgtaa agattccacc ttacctgtac caacgggcgg
      301 ggattcaata attatatttt taaagccatt atttgcaaaa gaatgcgcct gctgaagggt
      361 atcaacaaat accggagctt cgtgatcaaa attcgtttgg tatcgggcaa tccctgctgc
      421 tccgtgtgtg cctatattgg gaatgagttt acaggacgta tcctttaaat aggatttcgc
      481 attctctata ttggacttca atagcttgct atttactaat aaggctggag tgtctaattc
      541 gagatgatga tggccggtag gttttgatgt gggtctttcc atttataatg cccttgaaaa
      601 tatatcgatt aatatatcag aaagaggtac cttcatatat atggaacctt ttacaaagca
      661 aacacaggga actcaccaga tatacccctc agataagggc ctttcgtaca ccatcactgg
      721 gaaagacggt aaaagggaag ggtttataaa atatgattct gtagaatctg tttatgtgga
      781 atctatcagt agacaatcaa gattcacttt tgtttggctt gcaatgcttg tggtcttaat
      841 ttttatagct atggctgctt tagatgggtt tttgatgaaa atgattgcct tatctgtctt
      901 cactttaatg ggatcttttc tcttgtttga tcattactct caagaaatcc agccctgcct
      961 tgtaattcaa tctaatggat tttcggcgaa atttcctctt gagaaagatt tggctgacta
     1021 tgaattgagg gatttaatgg gtacagtact ggaatatata gacactcata attctataaa
     1081 caccagtact attagggttt ttattccgcg gtaagcccta ttctcagatt tggtacggca
     1141 tccatgtcta gttggaacgg aagcccgttt ttgaggttga agaaagcagc agctcctatc
     1201 atagctccat tgtctgtgca cagtggggct cgtggtacaa agatcggtat gtctataacc
     1261 tcgttcattc tttgccttag gcgactgttg gccgctacac caccaccgag aatgactgcc
     1321 ttactattgg tttcaagaac tgcttgtttg gtatttctta taaggcaatc cacaatagct
     1381 tcttgaaaag caaaggaata ctccgttgtt tgataggcac ttgggcctcg gttatcaaca
     1441 agcggataaa acccttcaga ctcagctcgc ctcaccaacg cggttttaag accgctaaaa
     1501 ctgaaatcca aagagttctt aatctgcggt ctgggcaagg ggtccatttc atctttagcc
     1561 ttctcagcca ttttttgaat ctcagggcct ccaggaaaac ctagaccaag caccctagca
     1621 gctttatcaa aggcttctcc ggcagcatca tctctagttt taccgagtaa ttcatactct
     1681 aaatgatctt tcatcaatat cagatcagta tggcccccag acgctatcag gcaaactata
     1741 ggaaactctg ccttggaatc cggcatcaaa tcttccaacc aagcggaata aatatggcct
     1801 tctaggtggt ttatgcctat taaaggtttg tcccaagaca ttgataatcc ctttgctgag
     1861 ttcacaccta tcaaaagaga tcctgctaag cctgggccat atgtgacagc aattgcatcg
     1921 atatctttgt gagtcatgtc agcttcattt agagcctcac ctacaactgg gataagcgat
     1981 gtgaaatgct ccctggaagc tatttcgggg atgatgccgc catatttttt atgcatgtct
     2041 gactgggatg cgacaatgtt tgagcgaata tatgttccat ccgttataac ggctgcagct
     2101 gtatcatcac aggaagtttc tattcccaaa acgttcatat tgatgagttt ctcaagttgg
     2161 atatcccatc acaaatagga aagtcttcat taaaaactgc actaccagct actactatat
     2221 ttgctccact ttgcactgcc tttagagtag tgtctggatt tataccgcca tcgacctgaa
     2281 taggtatgga aagcccttgg gattcaatct ctttagaaag tgtttcaatc tttttgagac
     2341 tttcgggtat aaagtcttgc ccgccggcac ctggttgtac tgacattatc aatatttggt
     2401 ctaccagagg aataatatcg taaatctttt caataggggt ttcaggattt aacgctacac
     2461 atttcctaac atcgaattta tctagcagtg ctaatgtgtc cttcaattcc ggactggcct
     2521 cataatgcac tgtaatagaa tcagcgcctg cagacgcaaa ctcagaaaaa tgattttcgg
     2581 ggcgtacggt catcatgtgt atatctaagg gcaaaccagt taaatctctc acagccttta
     2641 tgataggggt cccaaatgtt atggatggga caaaatgacc atccataaca tctatatgaa
     2701 tgccgtcggc cccgcaagtt tcggtctcct gtatttgctt gcccaactga gaaaaatcag
     2761 cagataaaat agatgggtac agcttaaatt caggcatctt gtttttccaa tttttgatag
     2821 ccgagtttga tagcctgttg aaccatctta aaggaggtgc cacctacagt atttcgagca
     2881 ttaaccgaag attctacact gacttcgtat acatcttcat caaataacat agaaaaaccc
     2941 cggtactctt ctaaagaaat ctcactgaat aatttatggt tagtggaagc atagtctgat
     3001 aattcactaa ttattccatg ggcttctcga aacggaactc ctttaattac taaatagtcg
     3061 gctaggtcag tcgctagaac catcccgccc tcggctgcct gtcgagcttt tactgaatct
     3121 acctgaatac cgggtagcat tccgaggaaa acttccaaga ctgccgctaa agtatcgtgg
     3181 ctgtcaaaaa aaccttcctt atcttcctgc aaatcccggt tgtaagtcat aggcaagcct
     3241 ttcaagacag tcaatataga aactaaatta ccgtacaccc tgccggtttt gcctcgagaa
     3301 atctcagcaa aatcaggatt tcttttttga ggcattatgc tactaccagt cgtgtattct
     3361 gcgggtaatt tgataagtga aaactcttct gtagtccaaa gaattagttc ttcagagaat
     3421 cttgaaatat gcatcataca tatagaagct gcggataaat aatctattac aaaatccctg
     3481 tctgaaaccg catccatact attttgagaa atctcagaaa atcctaactc tcgggcaaca
     3541 taagagcggt ctaaatcata aggaagccca gccaaggccc cgctacctag cggtaaaaca
     3601 tcagttcgtt cgtaggcttc tttaaatcgg gtcttatccc ttgtgagcat ttcaaaatat
     3661 gctaataaat gatgggaaaa aagcaccggc tgtgcctttt gtaaatgggt gtacccagga
     3721 atgattacgt cgttgttttg agatgcaatg tccaataaga tacgttgcag agttgtcagc
     3781 tgtctgataa tgatgtttat cgaatcttta acatacattc tcatatctag agaaacttga
     3841 tcattcctag aacgggcggt gtgcagctta ccggcaacgg aacctatttt ttcgtgtaat
     3901 cgagcttcga tattcatatg aatatcttct aattccggtc tccataagaa atttccagtt
     3961 tcaatttcag actctatttc taataatgct tctacaatgg aatccttatc agacaaagtt
     4021 attatttttt gtttgcaaag catagaagca tgaacaatag agccttttat gtcctgtttg
     4081 aacaatcgtc ggtcataatg caaagagaca gtaaaatctc ctaccttgtc tgagagtcct
     4141 ttaataactg gattttgatt tttgttgttg ctcatatacc cggacctgaa ctatttcttt
     4201 cttgcataca taatatacga caacgacttc ttacatagat aacataggat tatagtaacg
     4261 tagtacaaat aacatgacac aactcactca aaat
//
LOCUS       DASCNS010000288         5890 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002094296, whole genome shotgun sequence.
ACCESSION   DASCNS010000288 DASCNS010000000
VERSION     DASCNS010000288.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002094296"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(88..435)
                     /gene="rnpA"
                     /locus_tag="QF838_10095"
     CDS             complement(88..435)
                     /gene="rnpA"
                     /locus_tag="QF838_10095"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HJO61067.1"
                     /translation="MQKYQKLRNSSDFDSVKKYGTGSADFNLVMVAMDQAPAGEYKGS
                     RLGFIVSKRIGGATVRNKYKRRLKESANSISIIDGMDIVFIARNGILGSNFHGISSSM
                     YKLLADASLISTE"
     gene            complement(519..656)
                     /gene="rpmH"
                     /locus_tag="QF838_10100"
     CDS             complement(519..656)
                     /gene="rpmH"
                     /locus_tag="QF838_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HJO61068.1"
                     /translation="MPKRTYQPKNRRRMRVHGFRSRMSTKGGRNVLKRRMFKGRQKLT
                     V"
     gene            complement(1141..1521)
                     /locus_tag="QF838_10105"
     CDS             complement(1141..1521)
                     /locus_tag="QF838_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61069.1"
                     /translation="MSDLNLKQRIRAGEILIGVSVPLDASESEMEAIISKDDYAFAFT
                     DSQHGPFNEETLVRFCEASSNLGIPSQFRIKHTRHTYLIGNILDLGPVGIEVPQIETL
                     ETVQEAVRYFYYPQIRWQECRWDS"
     gene            complement(1573..2319)
                     /locus_tag="QF838_10110"
     CDS             complement(1573..2319)
                     /locus_tag="QF838_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HJO61070.1"
                     /translation="MSDLVWSVNPFGGAVIDPDSVSSLVLDFEIGLINAINKWRTEDI
                     KVAWLEIPKSAFKAIPRASEEGFLFHHATEEYAMMTLQVEENAFVPPYATHYIGIGGV
                     VINKDDELLVVSERYRASGRGPGYKLPGGALQPGEHLAEAAVREVFEETGISTTFQAL
                     TFFRHWHGYRYGKSDIYFVARLSPQDNEITMQEEEIAECIWMPVDQFLNEESVHLFNK
                     TIVRSATESEGLKVTPIDGYEPAEKFEFFK"
     gene            2449..3681
                     /locus_tag="QF838_10115"
     CDS             2449..3681
                     /locus_tag="QF838_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO61071.1"
                     /translation="MKYIIQSDRLIDGSGSIPAQNGAIVIENDKISKICATSELTEAD
                     KNQAEILSVPGGSILPGFIEMHSHIHCSSETDAYEHITTESNETFLLRGTQAVRSALS
                     SGVTTMRDLGSRNEVVFPLREGITNGIIPGPRLLVAGTPITTTGGHCNMFGTEANTSE
                     EVVKAIRQQFKLGADCIKIMSTGGGFTPGTNVRAPQYSTDILKKAVNDAERLGLKVAA
                     HCHAAEGVKNCVEAGIHNLIHCSWLATNPSELYDYDHDVADQIAEKGIYVDPTLALSH
                     LNKLRGKAIRPDSGAMRDPEKRFEILRDMWNRGVKFVTGMDSGMTNAHFDDFAYIPEV
                     MVNNMYISPLEAITCATKTSAECLGMEDEIGTLEAGKSADVIVVNGNPLEDITKLHDV
                     NTIISQGTLVKRENTLLI"
     gene            complement(3706..4785)
                     /gene="modC"
                     /locus_tag="QF838_10120"
     CDS             complement(3706..4785)
                     /gene="modC"
                     /locus_tag="QF838_10120"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HJO61072.1"
                     /translation="MPIEVNVTKKYGDFELMIQAAIGNGITGIFGPSASGKSTLLNCI
                     AGFEDPDDGLITLNSKTVFSSRAKTNVSPEKRRVGYVHQHSALFPHLTVKKNLEFGYN
                     LTEKYRRVGDLEDIVHLLQLEQIMDRGVINLSGGERQRVALARSLAASPDLLLLDEPL
                     ASLDMPFRGFILEKLREVSRGWNLDMIYVSHSVSEMIALADSVIVIRDGMKVVEGNSS
                     LLLNNSSVADYVDYASFENILHGVVDGSVDQNVSTLTIGDVKLITPKLTSEKGKQVTI
                     SIKASDIIVSKYPPVAISARNILKVSITNINHSENPTFIRCETGGCILTAALTMQSVS
                     DLGLRVSDEVYLIIKANSVTPMELY"
     gene            complement(4792..5460)
                     /gene="modB"
                     /locus_tag="QF838_10125"
     CDS             complement(4792..5460)
                     /gene="modB"
                     /locus_tag="QF838_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HJO61073.1"
                     /translation="MVDLILLSLQVAVIATLINIPIAIYVGWLIGRKNIKGKLILETL
                     ISLPLALPPVVTGYFLLILVGREAPLGQLSRFIFGADIVFTWVASAIAASVVSFPLMV
                     RAIIVAMQGVDTTLERSARSLGAGPLRTFLKITMPLCYNGLVAGILLGFVRAIGEFGA
                     TIVVAGNIPGRTQTIPLAIYSKVQLGKDSEALNLIFISTLMAIMTLIVHHWLIIRGSK
                     KRTG"
     gene            complement(5468..>5890)
                     /gene="modA"
                     /locus_tag="QF838_10130"
     CDS             complement(5468..>5890)
                     /gene="modA"
                     /locus_tag="QF838_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HJO61074.1"
                     /translation="VIVTNPQTAGLLDYSSLAELDLISLADPNLAPAGVYSREFLVNT
                     GLWSFMKDKFIFAADVRAALNYVKSGNVDAAIVYMTDGLTEPNLLLWDIVPPDSYGPI
                     SYPVAVIEAENRNQSGQSFVEFLVSPEVGDIFHSYGFR"
BASE COUNT         1783 a         1335 c         1094 g         1678 t
ORIGIN      
        1 tgagattatt tttttataag attttattaa acctaatact gtggatttaa caaaattatc
       61 tttgcggagt ttcaatctat ttcacgttta ctcagtggat attaggctag catcagccag
      121 cagtttatac atcgaagacg atattccatg aaaattggaa cctaagatcc cgtttctagc
      181 tataaagact atatccattc catcaattat cgaaatggaa ttagcagact ctttcaatct
      241 tcttttatat ttgtttctga ccgtggctcc tccaattctc ttcgagacaa taaaacccaa
      301 gcgagaccct ttatattctc ctgctggtgc ttgatccata gcaaccatga ccaagttgaa
      361 gtcagcagat ccggtaccgt atttttttac agaatcaaag tcggaggagt tacgaagttt
      421 ctgatatttt tgcatgtagt cactctaagt ccataagtcg accataaaac atgggcattg
      481 atacagtgac aaatttccta aggttaacga caataaaact atacagtcag cttctgacgt
      541 cctttgaaca ttcgtctctt aaggacattt cggccgcctt tggtactcat acgggatcga
      601 aatccatgca ctcgcatgcg acgcctgttt tttggttggt aggttctttt tggcattgat
      661 attcccttaa attaacctac aaattttact tacatcaata gaacagtgtc aatttattta
      721 gcagacggat attgaacttc ttttagccag ttattatttc ggatgctcca tcagaaccgt
      781 tacgcccata tttatgtact tatttctcag cgtgtgatca taacttctaa agctaattct
      841 tgagagcctt gtagttggtc taaggcatgc ttcaggcaat catcaactgt ttgaatcgga
      901 tgtttgggat gagcctcaat atcgtaaagt aagtcattag ggccccaggt aaggcaatca
      961 acaccattga tagcaagtta actcacagtg gtaactgccc ttagtgattc catttgtatc
     1021 gacaaaaatc ctgtttgatt ccaccacttg ccatactcaa ttctctcgaa ccctacctcc
     1081 ctagatccag acccacttcc atccttcagg ccgttgctat gataatcatg aattccgtac
     1141 ctagctgtcc catctacact cctgccacct tatctgtgga taataaaagt aacgaacagc
     1201 ctcctgaacc gtttcaaggg tttcaatttg aggcacttca atgcctactg gtcctaagtc
     1261 aagaatgtta ccgatcagat aggtatgcct agtgtgttta atacggaact gtgaaggaat
     1321 tcctaaatta ctggaggctt cgcaaaacct caccagagtt tcctcattaa agggtccatg
     1381 ctgactgtca gtaaaagcaa atgcataatc atctttactg ataatggctt ccatttctga
     1441 ttcagatgca tctaaaggca ccgaaactcc gattaagatc tctcctgccc gaattctttg
     1501 tttgagattc aaatcactca tttttatctc cttgaggcat tcaaaagtat ttgggccagg
     1561 tagcccaaat cattatttaa agaattcgaa tttctctgct ggctcatatc catcaattgg
     1621 ggttactttc aggccttcac tttctgtggc tgacctaaca atggttttat taaacaaatg
     1681 aacagactct tcattcaaaa attgatcaac cggcatccat atgcattctg ctatttcttc
     1741 ttcttgcatt gttatttcat tgtcttgtgg agataacctc gctacaaaat aaatatctga
     1801 tttaccatac ctgtacccat gccagtgtct aaaaaatgtg agagcctgga aggttgtaga
     1861 aattcctgtt tcctcaaata cctctctaac agcagcctcc gccaaatgtt cccctggctg
     1921 caaagcacct cccggcaatt tatatcctgg accccttcct gatgctctgt acctctctga
     1981 aacaaccaag agttcatcat ctttgtttat tacaacacct ccaataccta tgtaatgagt
     2041 tgcatatggg ggaacaaagg cattttcctc aacttgaagt gtcatcatcg cgtattcttc
     2101 agtggcatga tgaaataaaa aaccctcctc cgacgcccgg ggaatagcct taaaggctga
     2161 tttaggaatc tccaaccagg cgactttgat gtcctccgta cgccatttgt tgattgcatt
     2221 tattaaccca atttcaaagt ctaaaaccag ggatgacact gaatcagggt caataactgc
     2281 cccaccgaaa ggattcacgg accaaactaa gtcactcata tcgcctccat ttacttgagc
     2341 aaagggaagt atattgttgt gcccaaccca cggcaaccaa ccgcagatct ttggtgatta
     2401 gttgttgcta tataatgcct tcaagcttaa tcaaaatggg actcacaaat gaaatatatt
     2461 attcaatcag atagattaat tgacggttca ggttcaatcc ctgcacaaaa cggagcaata
     2521 gtcattgaaa atgacaaaat atccaagatt tgtgccacta gcgaattaac tgaagcagac
     2581 aaaaatcagg ctgaaatcct atcggttcct gggggatcta ttctccctgg atttatagaa
     2641 atgcattctc atatacattg cagttcagag accgatgcct acgaacatat tactactgaa
     2701 agcaatgaaa cttttttgtt acgtggtact caggcagtca ggtcagcact ctcttccgga
     2761 gtcacaacca tgagggacct cgggagtaga aatgaagtcg ttttcccact tagagagggt
     2821 atcactaacg ggataatccc aggtcctaga cttcttgtcg ccgggactcc cataactacg
     2881 acaggtggac actgcaacat gttcggaact gaagccaata cttcagaaga ggtcgtcaag
     2941 gcaattagac aacagttcaa acttggggcc gattgtataa aaataatgtc cacaggggga
     3001 gggttcactc ccggaaccaa tgtcagggcc ccccagtaca gtaccgatat attgaaaaaa
     3061 gctgtgaatg atgccgaaag actcggactt aaagtagccg cacactgtca tgcagctgaa
     3121 ggtgtcaaaa attgcgtgga ggctggtata cacaacctaa tacactgctc ctggttggct
     3181 actaacccat cagaactata tgactacgat catgatgtcg cagatcagat tgccgaaaaa
     3241 ggaatttacg tagatccgac tcttgctctt tctcatctca ataaattacg gggcaaggct
     3301 atcagacctg attctggagc aatgagagat cctgaaaaaa ggtttgaaat tctcagagac
     3361 atgtggaacc ggggtgtcaa attcgtcact gggatggatt ctggtatgac aaatgcgcat
     3421 tttgacgatt ttgcctatat cccggaggtg atggtaaata atatgtacat ttcccccctt
     3481 gaagccataa cttgcgcaac gaaaacatca gccgaatgtc ttggaatgga agatgaaatc
     3541 ggaaccttgg aagctggcaa atctgccgat gtcatcgttg taaacggaaa tccattagag
     3601 gatataacca aacttcatga tgtaaatacg attatttctc aaggtacact cgtaaagagg
     3661 gaaaatactt tgctcatata gcctcaaagg attatttttt gaggttcaat acaattccat
     3721 tggtgtgaca ctgttggctt ttataatcaa atagacttca tcagacaccc ttaagcccaa
     3781 atccgaaacc gattgcatcg taagtgctgc agtaagaatg caccctccag tttcacaacg
     3841 gatgaaagtt gggttctcag agtgattaat atttgtaata ctgaccttta atatgtttcg
     3901 ggcgctgatt gcaacaggcg gatatttgct gacgataatg tcagaagctt taatcgaaat
     3961 agttacctgc ttaccttttt ctgatgttaa cttgggagtg atcaacttaa catctccaat
     4021 tgttagggta gatacgtttt gatctactga accgtcaaca acaccgtgca gaatgttttc
     4081 aaaggaagca tagtctacat aatcagccac agagctatta ttcaacaaca aagaggaatt
     4141 accttccacc accttcatcc catccctaat cacgatgaca gaatcagcca aagcgatcat
     4201 ctctgaaaca gaatgagaca cgtaaatcat gtctaaattc catcctctag atacttcacg
     4261 aagtttttct aatatgaacc ccctgaaggg catatccaaa gaagctaatg gttcatctaa
     4321 gagtaataag tccggagaag cagccaagct tctggccaga gcaactcgct ggcgctctcc
     4381 tccagacaaa tttataactc cccgatccat gatttgctct agttgtagca aatgaacaat
     4441 gtcttccaaa tcccctaccc ttctatattt ttcagtgaga ttgtaaccaa attctaagtt
     4501 tttctttact gttaaatgag gaaataaagc agaatgctgg tgaacgtatc caactcttct
     4561 tttttccggg gatacgttag ttttagctct tgaagaaaat acagttttgg aatttaaggt
     4621 tatcagccca tcatcagggt cctcaaagcc agcgatacaa ttcagcaaag ttgatttacc
     4681 actagccgat ggaccaaaaa taccagttat cccattgcct atggctgctt gtatcatcaa
     4741 ttcgaagtcg ccatattttt ttgtgacatt tacttctata ggcaccgtaa attacccagt
     4801 cctttttttt gatcctctga tgatcaacca atgatgaact atcaaagtca ttatcgccat
     4861 taaggtggag ataaatatta aatttaatgc ttcggaatct ttcccaagct gtaccttact
     4921 gtatattgcc aggggaatag tttgagtcct tccgggaata tttcccgcca ccacaatagt
     4981 cgccccaaat tctccaattg ccctaacaaa acctagcaaa atcccagcca ccaacccgtt
     5041 gtaacacaac ggcatagtaa tttttaaaaa agttcttaaa ggtcctgccc ccagagatcg
     5101 cgctgatctc tctaaagtgg tgtcaactcc ttgcatagcg actatgatgg cccgaaccat
     5161 gagtggaaaa gacacaacgg aagctgctat cgctgatgct acccaagtga acacgatatc
     5221 tgccccgaaa atgaatcggg ataactgtcc caaaggagcc tcccttccca ccaaaatcag
     5281 caaaaaatac cctgtgacca ctggaggtag agccaagggc aaagaaatta aagtttctaa
     5341 aattaattta ccttttatgt tctttcggcc tattagccaa ccgacgtaga tggctatagg
     5401 aatattaatc aaagtcgcaa tcaccgcgac ttgtagcgat aacagaatta aatcaaccaa
     5461 atttcattca cctaaacccg taagaatgaa aaatatcacc aacttcagga gatactaaaa
     5521 attccacaaa tgactggcca ctctgattcc tgttctcagc ttcaataaca gctactggat
     5581 atgaaatggg accataggaa tcaggcggta ctatgtccca taacagtaag ttcggttcag
     5641 ttaaaccgtc agtcatgtaa acaatagctg cgtcaacgtt accagacttg acatagttga
     5701 gagctgccct gacatctgct gcaaaaataa acttgtcttt cataaaagac cagagaccgg
     5761 tattcaccaa aaattcccta gagtagactc ctgcgggtgc aagatttgga tctgctaaag
     5821 aaatcaaatc caactctgcc aagctagaat aatcgagcaa accggcagtt tgtgggttag
     5881 ttacaataac
//
LOCUS       DASCNS010000289         2498 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002095238, whole genome shotgun sequence.
ACCESSION   DASCNS010000289 DASCNS010000000
VERSION     DASCNS010000289.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002095238"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="QF838_10135"
     CDS             <1..416
                     /locus_tag="QF838_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HJO61075.1"
                     /translation="LPEGGAGPLAKQEGVEIREYDVIYHLIEDVSKALEGLLQPTYGD
                     VYVGRASVRAVFNVGRRVKVAGIFVSDGKISRDSKIRVLRGGSEIFMGPVSSLKHFKD
                     DVREVANGFEGGVSVDGFNDFEDDDVLEAYNTETI"
     gene            441..800
                     /gene="rbfA"
                     /locus_tag="QF838_10140"
     CDS             441..800
                     /gene="rbfA"
                     /locus_tag="QF838_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HJO61076.1"
                     /translation="MYHRMDSINSLLKKEISRVILEEVKDHRLAQVVSIVSVTTSRNM
                     QNATAHVTVMGDIKAKKDTISGLNSASRFIQRILRKDLSIKYIPSIRFVLDESLDEAE
                     KIRNIIDSISIETPDNT"
     gene            808..1731
                     /gene="truB"
                     /locus_tag="QF838_10145"
     CDS             808..1731
                     /gene="truB"
                     /locus_tag="QF838_10145"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HJO61077.1"
                     /translation="MSLPINGIINVNKPPGITSMDVIRRIRRVSGLRRVGHSGTLDPL
                     ASGVMVVALGVAARLLEYITELDKSYAAIIELGKSTDTYDGEGEIVNSESAVIVSDSD
                     VRSAIQTFKGTQSQIPPMHSAIKVNGVKLYNLARKGITIDRAPRQVNLYDAYISDFKS
                     PELELQITCSKGFYVRTFANDLGIRLGCGAYLKELVRTSVGKFSIEDSFNLDFIETNF
                     QKNNSRIFLHKLETVLDPIPKLQLNFDETQKVQNGISIPAEGNHKLEINSPISMALTE
                     EEKLVAVMRFNEEELIWKPEKVFRDQMQKIN"
     gene            1781..2101
                     /gene="rplU"
                     /locus_tag="QF838_10150"
     CDS             1781..2101
                     /gene="rplU"
                     /locus_tag="QF838_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HJO61078.1"
                     /translation="MNSFAIVETGGKQYRVHEGDTIRVESIEGDEGDTIDLESVKLIS
                     LDGETSVGTPNVEGAKVQAEVVNKGKGKKIVVFKYKAKTRYRRKNGHRQNFTDLRITD
                     ISVS"
     gene            2126..2380
                     /gene="rpmA"
                     /locus_tag="QF838_10155"
     CDS             2126..2380
                     /gene="rpmA"
                     /locus_tag="QF838_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HJO61079.1"
                     /translation="MAHKKGGGSTRNGRDSKSKRLGVKKYDTQVVTAGSILVRQRGTR
                     IHPGYNVGLGKDHTLFSLINGSVKFEYFPNGKKCASVYPA"
     gene            2393..>2498
                     /locus_tag="QF838_10160"
     CDS             2393..>2498
                     /locus_tag="QF838_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HJO61080.1"
                     /translation="MKSEIHPEYYNEATVVCSCGETFITGATKPELRVE"
BASE COUNT          848 a          463 c          581 g          606 t
ORIGIN      
        1 ctctcccgga aggtggtgcc gggcctttag caaaacaaga aggtgtggaa ataagggaat
       61 atgatgtcat ttaccatctc atagaagatg ttagtaaagc attggaagga ttgctccaac
      121 ccacctatgg agacgtatac gttggacggg cttctgttag agcggtgttc aatgtaggaa
      181 gacgggttaa ggttgctgga atatttgtta gtgatgggaa aatatcacgg gattcaaaaa
      241 ttcgtgtcct gaggggtgga tctgaaattt ttatggggcc tgtatccagc ctcaaacatt
      301 tcaaagacga cgtacgagaa gtggctaacg ggtttgaagg tggggtaagc gtggatggat
      361 tcaatgattt cgaagacgac gacgtactag aagcctataa caccgaaacg atctaaagtc
      421 gtttaggctc tggagtgact atgtaccacc gtatggatag tatcaactca cttctcaaaa
      481 aagaaataag ccgcgttatt ctggaggaag tcaaagatca taggttagcg caggtggtat
      541 ctattgtctc ggtaacaaca tctaggaata tgcaaaatgc gaccgcccat gtaaccgtga
      601 tgggggatat caaagcaaaa aaagacacta tttccgggct caactcagcc tctagattta
      661 tacaaagaat tctgagaaag gacttaagca taaagtacat ccctagtatc agatttgtat
      721 tggacgaatc gttggatgag gccgaaaaga tcaggaatat catcgattca atatctatag
      781 aaacccctga caatacatag gtccccaatg tctctgccta tcaacggaat cataaatgtg
      841 aataagccac ctggtatcac ctctatggac gttattcggc gaatacgtcg ggtaagtggg
      901 ctaagaagag taggacatag cggaacttta gatcctctag caagtggcgt catggtggta
      961 gccctaggag tagcagcacg attactggaa tatataacag aattagataa aagctatgct
     1021 gccataatag aattaggcaa aagtacagat acctatgatg gagaggggga aatcgtcaat
     1081 tccgaatctg cagtcatcgt aagtgattca gatgtgcgat ccgccattca aactttcaaa
     1141 ggcacccaat cgcaaatccc accaatgcac agcgccatca aagtgaatgg ggtcaaactc
     1201 tacaaccttg ctagaaaggg aattacaatt gacagagccc ctagacaagt caatctatac
     1261 gacgcgtaca tttctgattt caaatctcca gaactagaat tgcaaattac ctgtagtaaa
     1321 ggtttctacg ttagaacttt tgccaatgat ctgggcatac gactcgggtg cggtgcttat
     1381 ctcaaggaat tggttagaac atctgtaggg aaattcagca ttgaagactc cttcaacctg
     1441 gattttattg aaactaattt tcaaaaaaac aattcgagaa tttttttgca caagctagaa
     1501 accgttctag atccgatacc aaaactacaa ttaaattttg atgagaccca aaaagttcaa
     1561 aatggtatct caataccagc agaagggaat cataaattgg aaataaacag ccctatttcc
     1621 atggccctta cggaggaaga aaagctcgta gcggtcatga gattcaatga agaggaactg
     1681 atctggaaac ctgaaaaagt atttcgtgat cagatgcaaa aaattaattg acgttaccca
     1741 taaaggatag atacaattat taatcgtgaa ggtgatataa gtgaacagtt ttgcaatagt
     1801 agagaccggc ggaaaacagt acagagtcca tgaaggagat accatccgag tggaatcgat
     1861 agaaggtgac gagggtgaca caatcgacct tgaatcagta aaactgattt ctcttgatgg
     1921 tgagacatcc gtgggaaccc ccaatgtgga aggagctaaa gttcaagcag aggtggttaa
     1981 caagggtaaa ggaaagaaga ttgttgtatt caaatacaag gccaagactc gttacagaag
     2041 gaaaaacggt caccgccaaa attttactga tctaagaatt actgacatat cagtcagcta
     2101 agacacaacc gcaggagtaa gacccatggc ccataagaaa ggtggaggaa gtacccgtaa
     2161 tggaagagat agtaaatcta aacgattggg cgttaaaaaa tacgatactc aggtagtcac
     2221 tgcaggatct atattggtca ggcaaagagg taccagaata catccagggt ataatgtggg
     2281 actaggcaag gatcacactc tgttctcatt aattaatggt tcggtgaaat ttgaatattt
     2341 cccgaatggt aagaaatgcg ctagcgttta cccagcctag gaactaaaca gaatgaaatc
     2401 cgaaatacac ccagaatatt acaatgaagc tacagtggtt tgctcttgtg gtgaaacctt
     2461 cattacggga gcgactaaac ctgaactaag agtagaaa
//
LOCUS       DASCNS010000290        13537 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002095901, whole genome shotgun sequence.
ACCESSION   DASCNS010000290 DASCNS010000000
VERSION     DASCNS010000290.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002095901"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(124..417)
                     /locus_tag="QF838_10165"
     CDS             complement(124..417)
                     /locus_tag="QF838_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61081.1"
                     /translation="MKLGLMALAALTAAIIFVGCGTAVGRATGADAEPINTNTSAGSL
                     PVITEEKLIVVNENLASQEALLVDQEKALDKHQAELQNLKEIVTAFESHSAKN"
     gene            complement(588..1127)
                     /locus_tag="QF838_10170"
     CDS             complement(588..1127)
                     /locus_tag="QF838_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61082.1"
                     /translation="MPKTKENVAQMQYAYQIWNQKTSTRKDIYQKMVRKFGTRTTISF
                     STLNRWLLQYDALPEDEVMQDGPYEWKWLAHYGIPWQEGSLADELYREYQLRTKTYPT
                     GRHMKWLWRQWHLEGQGFRPRELPNTDDTTFFWEQLFKHVHKRVEEEKIEYMARKLNW
                     GNVHNPKFLDQTHSKTKDP"
     gene            complement(1407..1994)
                     /locus_tag="QF838_10175"
     CDS             complement(1407..1994)
                     /locus_tag="QF838_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61083.1"
                     /translation="MTPNLKISAKQKTQAYILYSSGHSQHSIIEQLKKHFEENTISLR
                     ILSRWISSFKKLPESATKLDEPFQWNKCDDYGIPWTNSLKLLELCHHYYEREDKTPSA
                     RQAKWWWKVSQAGPDLRANQIAELGNLYSDREVEGMVTGDAPVLDDLNAYITYKPYHT
                     NRVRTYARFINMNGIKPLKPKSDESNTTGGLKNTL"
     gene            2098..2619
                     /locus_tag="QF838_10180"
     CDS             2098..2619
                     /locus_tag="QF838_10180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJO61084.1"
                     /translation="MTERFFYYLIYAMALDYKTKIVSTSVELEDVLELRVEVFVLEQS
                     VPAIEEIDELDTMKAIKSGRAIHVLVSKGQLAVGTARLILEGSIPGSEKISELPHIGR
                     VAVKKITRRQGIGEILMVRLHQLAKTMGFRGVTLSAQMQAASFYTQLGYSARGAVYDD
                     VGIPHQDMDLIFD"
     gene            complement(2638..3963)
                     /locus_tag="QF838_10185"
     CDS             complement(2638..3963)
                     /locus_tag="QF838_10185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HJO61085.1"
                     /translation="MLYDAESGNLSITAGGDAMITRKLSVHKEENFLDLIELFRSSDV
                     GYVNLEMLMHDFEHSPGSAGGTFTGSDPSNLAELTWSGINLVSTANNHSHDYGEGGVL
                     TNLKHLKTSDLTHAGTGQHLSEARSAGYLDTHSGRVALVATASTFAESGRALDQRPDL
                     KGRPGLNPQRFKTTYTVDQDSFDQLRRTNRLLGLEQKRNFQRGFRPSGVIPADTETEF
                     SFLETRFMVGDYFSENTELNKEDLSENLKWISDAKRMSDWVIVSFHCHESGKTMSEPP
                     EFLINFAHECIDAGASVFIGHGPHVTRGIEIYKGAPILYSLGNFIFQNDTVKWQPSYN
                     YDQIGLDHTFTPADFYDKRSDEGKRGFPGDPIYWQSVVAQIKFENGNLSSLDVVPIDM
                     GYGKPRSMTGRPLIASGKIAAQALSRMKDLSEDMGTDISIVGNRGIVTI"
     gene            complement(3997..4551)
                     /locus_tag="QF838_10190"
     CDS             complement(3997..4551)
                     /locus_tag="QF838_10190"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HJO61086.1"
                     /translation="MTEPNVELRKVAREDILRINQWLADDEVAENWFGRYSYGNPAHL
                     GYHPEQIDTFSDQQWSETFSNPEHAIFSIYTTDGSHIGETHIAIEESLGDGQISILIG
                     AREMWHHGFGTAALHATLNQAFTHYELFRVWADIPEYNEVALKMFEHFGFKHEGTLRK
                     SRPHEGARYDSIVMGMLSTEYNVD"
     gene            4787..5857
                     /gene="prfA"
                     /locus_tag="QF838_10195"
     CDS             4787..5857
                     /gene="prfA"
                     /locus_tag="QF838_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HJO61087.1"
                     /translation="MLERLSSVEEKYEELNKLLSDPEVVADYTKVQQYAKEQSVMREV
                     VKLAREYREVIKEISELRDMLRSESDPELLSLAKDEQATLEGKKENAEEKLRRALVPK
                     DPNDDKNVIIEIRAGTGGEEAGLFAQNLYRMYSRLAQRLNWNLEVINSNSTGIGGLKE
                     ITFQVGGDQAYSKLKHESGVHRVQRVPTTESSGRIHTSAATVAVLPEAEEVDVEINQE
                     DLQIDIYHASGHGGQNVQKVATAVRITHIPSGVVTTCQDERSQHKNKEKALAVLRSRL
                     LAAEIEKQQQEITDNRRSQVGSGDRSERVRTYNFPQGRITDHRIGLTSYNLEQVLDGD
                     LMEFIEALVQEDQARKLQNASL"
     gene            5854..6702
                     /gene="prmC"
                     /locus_tag="QF838_10200"
     CDS             5854..6702
                     /gene="prmC"
                     /locus_tag="QF838_10200"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HJO61088.1"
                     /translation="MNVKETLNVASEELMKIGSLDARLEAEVLLRHVLKIDRATMFRD
                     LDEDVSIEDSGNIASLVKRRTQREPLAYITGAKEFYGLPFVVSEEVLIPRQETELLVD
                     TAIVQAKSLDKSEISIVDVGTGSGAIAVSLALNIPTSSVIAVDISESALTIADDNRRA
                     HGLYSRVKLRRGNLLEPIVEKIDILVSNPPYIRSDKLTSLQEEVLQEPMVALDGGYDG
                     LELIKKLLFQAVDKMSNPSVILFEIDSDQAAEAVKLSQQFFPSAIITVLKDLSNNERA
                     VLLEIR"
     gene            complement(6762..7895)
                     /locus_tag="QF838_10205"
     CDS             complement(6762..7895)
                     /locus_tag="QF838_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HJO61089.1"
                     /translation="MSNLRELRRTYGFDEVAIAPGTVTVNPEMTDTSFKLGDVSINIP
                     ILGSAMDAVANPYFIGKMHEQGALSVMNLEGVQTRYEDPEGVLAEIAKTPNDMVTAVM
                     QKVYSSPIKEALIGRRVEEIKATGSKCSVSVTPAVTKSYAPLAVEAGADMIVVQSTVT
                     TARHSSNSIKGLQFPELLEMVKVPILVGNCVGYEVATEIMETGIHGILVGVGPGAACT
                     TREVTGVGIPQVTATMDCALARDDFFQRTGKYIPIITDGGIRTGGELCKAIASGADAV
                     MIGTPLAQSKEAPGQGFNWGMASPHPALPRGTRVNVGVKGTLKELFYGPSSVSNGTQN
                     LVGALKACMGMVGARNIKDMHSVEVVVAPSIKTEGKHYQLGLE"
     gene            8174..9346
                     /gene="queG"
                     /locus_tag="QF838_10210"
     CDS             8174..9346
                     /gene="queG"
                     /locus_tag="QF838_10210"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HJO61090.1"
                     /translation="MPLSNGNLEDQVRECAFSLGFDLVGITDSEPFKSNEKAAMKRIN
                     DGHMEGYHWYTKERVRKMNRPQLLLDNAKSVISLATSYLTMDPDTGTFKNGRVARYAW
                     GDDYHKVLKSKLKEFCIKLQDISGKDINTRIFVDDGPMNDRAAARRSGVGWFGKNTNI
                     LTPTHGSWVFLSQVITDLYLKPDNPLKKTCGNCVKCIVDCPTGAIVAPYVIDNEKCIS
                     YLTIELRGVIPRKLRTLMGDWIFGCDICQDVCPVNKKALKGNLGAFQQRPGFSTPDLV
                     EILNMDQAEFSRKYKDSPIKRTKLVGLKRNACVALGNNGDPDAITALSIALKTLESLV
                     RIHAAWALGRIGGRESLVVLKEALQFEEDQDTLDEIQMSIGEIGIPLSGEELKNVR"
     gene            9336..10097
                     /locus_tag="QF838_10215"
     CDS             9336..10097
                     /locus_tag="QF838_10215"
                     /inference="COORDINATES: protein motif:HMM:NF037411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61091.1"
                     /translation="MSGKIYVINVGSNASHSFCSPIFSDQTFEFIPIPEDRQLPSSHG
                     TEYRNLKSHYDPEQDLSSYIPKNMFGATVHSDPEFDTYTYGDNCEVNPRAMGMKNVTR
                     GDFLLFLSRLQYWEAGEPTDNFGFYFIGFIHVDQILQSVWAPPSELQMERFNVNAHIR
                     RGMADEDLWDGFWVFGGSSWSRRFYKAVPVTRNLCEQVFVAANGSPWEWKEKRTELQT
                     IGSYTRSCRCAIDPAVGNGKVRADILWDWVEKYSR"
     gene            10222..11013
                     /locus_tag="QF838_10220"
     CDS             10222..11013
                     /locus_tag="QF838_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HJO61092.1"
                     /translation="MDIEDPCVRCGFNCTCAIRAVGACVEFNGSLVLDDVTFSVGKGV
                     LSALVGPNAGGKSTLFNAIAGIQDIVHGSIFVNGLPPKDSKGSISYIPQRENVNWRFS
                     LTALEVVDLGLVKNNSLLAFSRKGSLEKVRSALERVDMWNYKDQLISQMSGGQRQRVF
                     IARALCQEANILLLDEAFSGVDVASQFGLIDMLRELKEQGKTIIIATHDLNTLADRFD
                     EVICLNRHVCAQGSPDTAFTPEVLEELYGSHPGMFLSHSLGHHHD"
     gene            11006..11845
                     /locus_tag="QF838_10225"
     CDS             11006..11845
                     /locus_tag="QF838_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HJO61093.1"
                     /translation="MINIVLEPFQYDFMLRALFVSSMVGIICPILGAYVVIRGMGFMG
                     DAMAHAVMPGIVIALILGLSPFLGSVPMAIVVAVSVGYLIHKKDVSVDTAVGVMFAGL
                     FSLGLVLMSLVGGLAISIEDILLGQILGVSRVDMLISFILTVLVLVALVTFHKQLIFS
                     GFDPVGSVVAGLNAGALNYMFLILLGISIVVTLHVVGVVLVVGLLITPAAASQMVMRR
                     FTRAMLLGIVFGLFSTVVGLYLSYYFDLPSGPVMTLISFAIFLCCAVYEKFFSQTQTS
                     HLM"
     gene            complement(11818..12243)
                     /locus_tag="QF838_10230"
     CDS             complement(11818..12243)
                     /locus_tag="QF838_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HJO61094.1"
                     /translation="MTNSNSNTTRPNLLALLEDHGLRITAPRQRIVDYIHAAAEWFTA
                     EQMADDLSNVSRATVFRTLKILLEANVICRLSIPGGNPRYSLSQVDHHHHTVCVSCGK
                     VGEFKVSTVEKLLKGIESDIPGSIVGHNIEFYIKCEVCV"
     gene            12397..>13537
                     /locus_tag="QF838_10235"
     CDS             12397..>13537
                     /locus_tag="QF838_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO61095.1"
                     /translation="MTSRKEYSPTDSDQTTNTSMNPGQNRFKFFYGWWICIGGAFVMA
                     MSSGINFHGFGNFIIPLSKEFGWSRTTVSAVFSLARLEAGFIGPVEGWAVDRLGPRKL
                     MLVGIPVMGLGFILLSQVSGLFTFMLVYILGITLGNSLGMHVPASAAVANWFNRKRGL
                     AFGIMWSGVGLGGLLVPALGWAIDVYGWRDASVYVGIFVIFVGVPVAALMRHRPEQYG
                     FLPDGQIIPQKTSNGKLGKNEDLEVDFTAREALQTSSFWFLSLSIMARSLVTGGVGLH
                     LVPYFVGLGASPIEAAAYAGSVGVISIPGRFGLSYLGDYFNRRYMMAISLLFMTLSIV
                     LLATADSLSSSIPGLVAYSISQGGISVIPQSIIADYFGRKAFATIS"
BASE COUNT         3684 a         2585 c         3032 g         4236 t
ORIGIN      
        1 agaaatttct tcttcgagat gttgtccctg cgcagccatg tcattttcta attggtttga
       61 catagttgaa acttcggctg cgacagattt aatttcccta tctgcctaac tttctgtatt
      121 ggcttaattt tttgctgaat gtgattcaaa ggcagtaact atttctttaa gattttgcag
      181 ctctgcttga tgtttatcca gtgccttttc ctggtctact agtaatgctt cctggcttgc
      241 caaattctcg ttaaccacaa tcaacttttc ctcggttata acaggtaggg aaccagccga
      301 ggtgtttgtg ttgatgggtt ccgcgtcagc gccggtagca cgccctactg ctgtaccgca
      361 acccacgaat attattgcgg cagtaagagc cgcaagagcc attagtccga gtttcatagt
      421 tactacctcc aaaattttgt atttgttaag caattgagtg attctgtagt ttaacttatg
      481 gaaattaaaa agatagggta ttttcacttg ttcctattta cttcaaacta gtagtaatag
      541 gcggcattga ttgcaggtca tagcagcact ttttgtttac gatcggttta tggatctttt
      601 gtttttgagt gtgtttgatc tagaaacttg ggattatgga catttcccca atttagtttt
      661 cttgccatat attcaatttt ttcctcttct actcttttat gaacatgttt aaaaagttgt
      721 tcccagaaaa aagttgtatc gtcagtattt ggcagttccc gcggcctaaa gccttgccct
      781 tctaagtgcc attgtctcca taaccacttc atgtgtcttc cggtggggta agttttagtc
      841 ctcagttggt attctctata aagttcgtca gctaaactcc cttcctgcca gggtattcca
      901 tagtgtgcca accacttcca ttcgtagggt ccatcctgca taacttcgtc ctcaggtagg
      961 gcatcgtatt gtaacagcca cctatttaac gtggaaaagc taatagtcgt cctcgttcca
     1021 aatttcctga ccatcttttg ataaatgtct ttccgggtag atgtcttttg attccatatc
     1081 tgatacgcat attgcatttg agctacgttt tcttttgttt taggcattgc agattctcat
     1141 ataaaattat tatttatcaa tcatggatta ccaataacca ctatttattg ttttatagat
     1201 ttaaattcat attaacacca agtatcatat atgagtaaca aacaacctgg tttggagttc
     1261 aaaaaaagta ttgcccccat tgttgataaa acagtaattc gaagtggggg aaactaataa
     1321 tacatatcct ctcgtgttag aggatatgtt gatgggtacc agggtcatat gaaagatttc
     1381 aactggggtt attttccaac gaatttctac aaagtatttt tgagcccacc tgtggtgttg
     1441 ctctcatcac tttttggttt caagggtttt atcccattca tattgatgaa acgagcgtag
     1501 gtcctaacac gattggtatg atacggcttg tacgtgatat aagcattcaa atcatcaaga
     1561 acaggtgcat ccccagtcac catgccttct acttctctgt ctgaatatag atttccaagt
     1621 tctgctattt ggtttgctct aagatcgggg cctgcctgac ttactttcca ccaccatttt
     1681 gcctgtcttg cagatggtgt tttgtcttcc ctttcataat agtggtgaca taactccaat
     1741 agttttaatg aattggtcca aggtataccg tagtcgtcac atttattcca ttggaacggt
     1801 tcatcgagtt ttgtggctga ctcaggtaat tttttgaagc tagatatcca acgactaagt
     1861 attcttaaac tgatcgtatt ttcctcaaaa tgttttttta actgttcaat tattgagtgc
     1921 tgactatgac cagacgagta gagaatgtat gcctgagttt tttgcttcgc acttattttt
     1981 aaatttggag tcaagatttt ctcctctcca gactttgact tatttcaagc tgaatatgtg
     2041 aaattgaaaa ctaattatta aatgttatcc cctatttgcc ttatctttca tagtgaattg
     2101 actgaaagat ttttttacta tctaatttat gctatggcgc tagattacaa gactaaaatc
     2161 gtttctactt ctgtagaact tgaagatgtt cttgaacttc gagtggaagt tttcgtgttg
     2221 gagcaatctg ttcccgccat tgaagagatt gatgaattag atactatgaa ggcgattaaa
     2281 tctggtcgag ccatacatgt attggtatct aaaggtcagc tggcggtagg gacagctaga
     2341 ttaatactgg aaggcagtat acctggatct gagaaaatct cagaattgcc tcatatcgga
     2401 cgagttgcgg ttaaaaaaat tacgagacga caaggaattg gtgagatact catggtgaga
     2461 ttgcaccaat tagccaagac aatgggcttt agaggggtca ctctttcagc tcagatgcaa
     2521 gccgcttctt tctatactca attgggttat agtgctagag gggcagttta tgacgacgtt
     2581 ggtattccac accaggatat ggatttgatt ttcgattaat cagtagatga aattagtcta
     2641 aattgtgacg attccgcggt ttccaacgat tgaaatgtcg gttcccatat cttcagacaa
     2701 atctttcatt cttgataaag cttgagctgc gattttgccc gaagcaatca aaggccttcc
     2761 tgtcattgag cggggctttc catatcccat atcaatcggt acaacatcca gagaggagag
     2821 gtttccattt tcgaatttga tttgtgccac tacactttgc caatatatcg gatcacctgg
     2881 gaatcctctt ttcccttcat cgctacgctt atcgtagaaa tctgctggtg tgaaggtatg
     2941 gtctaagcca atttgatcat aattatagga aggctgccac ttgacggtgt cattttggaa
     3001 aataaaatta cccaggctat ataaaattgg ggcacctttg tatatttcga ttccccttgt
     3061 gacatgcgga ccatgcccga taaagacaga tgcacccgca tcaatgcatt catgggcgaa
     3121 attaataaga aattccggtg gttccgacat agttttcccg ctttcgtggc agtgaaatga
     3181 aactatgacc cagtcggaca ttctttttgc atctgagatc cattttaagt tttcactaag
     3241 atcttcttta tttaattccg tattttctga gaagtaatcc cctaccataa atcgggtttc
     3301 aagaaaggaa aattctgttt cagtgtccgc tgggattact cccgatggtc tgaaacctct
     3361 ttgaaagttt cgtttttgct cgagtcctaa gaggcgattg gtccttctga gttggtcgaa
     3421 ggaatcttgg tccactgtgt atgtggtttt aaatcgttgg ggatttagtc ccggcctacc
     3481 ttttagatcc ggtctttggt ctaaagctct acccgattct gcaaaggtag aggcagtagc
     3541 taccagtgcg actcttccac tgtgggtatc aaggtatccg gcactcctag cctcacttaa
     3601 atgttgacca gtgccagcat gggttagatc cgaagtctta agatgtttga gattcgttag
     3661 gactccacct tctccatagt catgactatg gttgttggcg gttgatacta aattaatgcc
     3721 tgaccaggtt aattcagcta gatttgaagg atcagatcct gtgaatgttc cacctgcaga
     3781 tccgggggag tgttcaaaat catgcattag catttcaaga ttcacatatc cgacatctga
     3841 tgaacggaat aattcaatta ggtctaagaa attttcttct ttgtggactg aaagttttct
     3901 ggttatcatg gcgtccccac cagcagttat agacaggttc ccagattcag catcataaag
     3961 catagttaat tcctgttaat ttaatttgga atctatttag tcaacgttat attcggttga
     4021 caacataccc ataacaatgg agtcgtacct tgccccttcg tgaggtcgac tctttctcaa
     4081 agttccttcg tgtttaaaac caaaatgttc aaacatttta agagcgactt cattatattc
     4141 aggtatatct gcccatactc tgaataattc atagtgggta aacgcttggt ttaaagttgc
     4201 atgtaaggct gcggtcccaa aaccgtggtg ccacatctcc ctggccccaa ttaggatcga
     4261 tatttgccca tcgccaagtg attcctcaat ggcgatgtgc gtttccccta tatgagaacc
     4321 atcagttgtg tagatggaaa atatggcgtg ctccggattt gaaaatgttt ctgaccattg
     4381 ctggtcggaa aaggtgtcaa tttgttccgg gtggtatccc agatgggcgg gattaccgta
     4441 tgagtatctt ccaaaccaat tttcggcaac ttcgtcatct gccaaccatt gatttattcg
     4501 taatatatct tccctagcaa cttttcttaa ttcaacgtta ggttcagtca tacaaacctc
     4561 actatttgta atggacatgt ttgcaagatg ccatagatga tcgcatttac agagaattat
     4621 ttaaaattga gcgggatacg tcaataaacc taaaattgtt gaatttctgt tcatgaacag
     4681 aaactacaca tatctaattc caaaattatg gaaatcgaga taagttcaag ttatgattac
     4741 tctttgatct caaagttatc acaaactcaa ctctcaggat gaattgatgt tagaaaggct
     4801 ttcctcagtt gaagaaaagt atgaggagtt gaataaatta ttgtcagacc ctgaggtggt
     4861 agctgattac actaaagttc agcagtatgc taaagaacag tcggtgatga gagaagttgt
     4921 gaaacttgct cgagagtaca gagaagttat taaagaaata tctgagttgc gagatatgct
     4981 cagatctgaa tcggatcctg agctattatc gctggctaaa gatgaacagg cgactcttga
     5041 aggaaagaag gaaaatgccg aagaaaaact tcgtagggct ttagttccaa aagatcctaa
     5101 tgacgataaa aatgtaatca ttgaaatcag agctggaacc gggggggaag aggcaggctt
     5161 atttgcccaa aacttatata gaatgtactc tcggttggcc cagcgattaa attggaattt
     5221 ggaggtaata aattcaaatt ccacggggat tggggggtta aaggaaatca cttttcaagt
     5281 tggaggtgac caagcctaca gcaaactgaa gcatgaaagt ggtgttcaca gagttcagag
     5341 agttccgacc actgaatcta gcggaaggat acatacatct gctgccaccg tagctgttct
     5401 tcctgaagct gaagaggttg atgttgagat taatcaagag gatcttcaga tagatatcta
     5461 ccacgcgagt ggtcatggtg gacaaaatgt acaaaaggtt gctactgccg ttcgaattac
     5521 acatattcct tccggtgttg taacaacatg ccaggatgag aggtctcaac ataaaaataa
     5581 ggaaaaagct ctcgcagttc ttaggtcaag attacttgct gctgagatag aaaaacaaca
     5641 acaagagatt accgataacc gaaggtccca agtaggctct ggagatcgtt cagaaagggt
     5701 tcggacatac aattttccac agggtaggat tacagaccat aggatagggt tgaccagcta
     5761 taaccttgag caggtactgg atggcgattt gatggagttt atcgaagctt tggtccagga
     5821 ggatcaggct cgtaaattgc agaatgctag tctatgaacg ttaaagaaac ccttaatgtt
     5881 gccagtgaag agctaatgaa aataggctca cttgacgcta ggctggaagc ggaagtatta
     5941 cttaggcatg ttctaaagat tgacagagct actatgttca gggatttaga tgaagatgtc
     6001 tccattgagg attcaggcaa tatagcttcc ttggtaaaaa gaaggactca gagagagccc
     6061 cttgcgtaca ttaccggggc caaggaattc tatggattac cgtttgtcgt gtcagaagag
     6121 gtgttgattc caaggcaaga aacggagctt ttggttgata cagctatcgt tcaagccaag
     6181 tcgttggata aaagcgaaat ttctatagtg gatgtcggaa caggcagtgg agctatagcg
     6241 gtttcattgg ctttgaatat tcctacttct agcgtaatag ctgtagacat tagtgaatca
     6301 gctttaacaa ttgcagatga taatagaagg gctcatgggt tgtactcaag agttaaactg
     6361 aggcgtggta acttacttga accgatcgta gaaaaaatcg atatattggt tagtaatccg
     6421 ccttacatca ggagtgataa attgacctcc ctccaggaag aggtattaca ggaaccgatg
     6481 gtggctttgg atggaggata tgatgggctg gagttgataa aaaaattact ctttcaagcg
     6541 gtagacaaaa tgtccaatcc atcggtcata ttgtttgaaa tagattctga tcaagcggct
     6601 gaagctgtca aattatctca acaatttttt ccgtctgcca taattacagt tttgaaagat
     6661 cttagtaata acgaacgggc agtgctttta gaaatcagat aggtgcttat tcatatatgt
     6721 cgagtaagtc tgctagtgta tgtaattaat cctatcgacc cttactctag accaagttgg
     6781 taatgtttac cctcagtttt tattgaggga gccactacga cttctacaga atgcatgtct
     6841 ttgatattcc gcgctcctac catgcccata caggctttta gtgcacctac caaattttgg
     6901 gtaccatttg atacagagga aggaccataa aacagttctt taagagttcc tttgacacca
     6961 acgttaaccc tcgtacctct aggcaatgct gggtgaggac tagccattcc ccaattaaat
     7021 ccctgccctg gcgcttcttt tgattgagcg agaggagttc ctatcatcac ggcatcggct
     7081 ccacttgcga ttgctttaca aagttcaccc ccagttctga tccctccgtc ggttatgata
     7141 ggtatgtatt tgcccgttct ctggaaaaaa tcgtctcttg ccagtgcgca atccatggtg
     7201 gccgtcactt gaggtatgcc aactcctgtg acctctctgg tagtacatgc agcacctggt
     7261 ccaactccaa cgaggatacc gtgtatacca gtctccatta tttccgtggc gacttcgtac
     7321 cctacacagt tacctactaa aatcggcact ttcaccattt cgagcaactc cggaaattga
     7381 agacctttta tgctgttgga agaatgccga gcagtagtaa ctgtcgattg aaccacaatc
     7441 atatctgctc ccgcctcaac ggcaagtgga gcatatgact ttgtaaccgc tggggttaca
     7501 gaaacggaac attttgaacc tgttgctttg atttcttcaa ctcttcttcc gatcaacgcc
     7561 tcttttatcg gtgaggaata gactttttgc attacagcag taaccatgtc atttggagtt
     7621 ttcgctattt ctgcaaggac accttcagga tcttcatacc gagtttgtac cccctccaag
     7681 ttcataacgc ttaaggcacc ctgttcgtgc attttgccta taaagtaagg gttggccacg
     7741 gcatccattg ctgaccctag aatcggtatg ttgatactca catcacctaa tttaaatgag
     7801 gtatctgtca tttcagggtt aactgtcact gtcccggggg ctattgccac ttcgtcgaat
     7861 ccataggttc ttcttaattc gcgaagattt gacatttcca gctcctcaaa ttgaattcat
     7921 ttaaatgaga gacattctaa attttctttg ggatggaatg ttcaaaaatt tatctgaata
     7981 atcttaatgg tttatccact aggcgaatgt gattagaatg gttcgtagag ctgaaaaccc
     8041 aatctgaccc gttggtcaat agatgctcca taataacaag atacgtatag tgggatgcgg
     8101 tgatgatatg gctgaaatgc gagaggtgtc aatacgatag cacggatatc tataattcaa
     8161 agggagattt aaattgcctc tcagtaatgg aaatctagag gatcaagtta gagaatgtgc
     8221 cttttctctt gggtttgatc ttgttggtat aacagattct gaacctttta aaagcaatga
     8281 aaaggccgcc atgaaacgaa taaatgatgg gcacatggaa gggtaccact ggtacacaaa
     8341 agaacgggtc cgaaagatga atcgcccgca acttctttta gataatgcta aatcggtaat
     8401 atccctagcg acaagctacc taacaatgga tccagataca ggaacattta agaatgggcg
     8461 agtagcaagg tatgcatggg gagatgacta tcataaagtc cttaaatcta aactaaagga
     8521 attttgcata aaactacaag atatctctgg taaagatata aacactcgta tttttgtaga
     8581 tgatgggcca atgaatgata gggctgctgc acgaaggtcc ggggtaggtt ggtttggtaa
     8641 aaacactaat atattgactc caacccatgg ctcatgggtt tttctatccc aagtgattac
     8701 tgatttgtat ttaaaaccag ataacccact aaaaaaaact tgtggcaact gtgtgaaatg
     8761 tatagttgac tgtccaaccg gagcaattgt agccccgtat gtgatagata atgaaaaatg
     8821 tatctcttat ctaaccatcg aactccgagg tgtcattcct aggaaattga gaaccctaat
     8881 gggggattgg atttttggtt gtgatatctg tcaggacgtg tgccctgtta ataaaaaagc
     8941 tttaaagggt aatcttggcg cgtttcaaca aagacccggc ttttctacac cagatctggt
     9001 tgagatattg aatatggatc aggctgaatt tagtagaaaa tataaagata gtcctataaa
     9061 aagaaccaag ttggtcgggt taaaaagaaa tgcatgtgtt gctttaggga acaatggtga
     9121 ccccgatgca ataacggcac tatctatcgc tttaaaaacc ctcgaaagtc tagttcgcat
     9181 acatgccgct tgggcgttag gaaggatagg ggggagggaa tcccttgtag tactaaaaga
     9241 agcactccag ttcgaagaag atcaggacac gctagatgaa attcagatgt ctattggaga
     9301 aattggcatt cctctgtcgg gcgaggaatt aaaaaatgtc cggtaagatt tacgtcatta
     9361 atgttgggtc taatgctagt catagtttct gtagcccaat ctttagtgat cagacctttg
     9421 aattcatacc gataccagaa gataggcaat tgccttccag tcatgggacg gaataccgaa
     9481 acttaaaatc gcattacgat cccgaacagg atttatctag ctacattccc aaaaacatgt
     9541 ttggagctac tgtgcacagt gatcctgagt tcgatactta cacatatggg gataattgtg
     9601 aggttaatcc acgggcaatg ggaatgaaaa atgtaacaag aggagacttc cttttgtttt
     9661 tatccagatt gcaatattgg gaagcaggtg aaccaacaga taatttcggg ttctatttta
     9721 ttggctttat ccacgttgac cagattctgc aatcagtctg ggcgcctcca tccgaattgc
     9781 agatggagcg ctttaatgtt aacgctcata taagacgtgg aatggcggat gaggatctct
     9841 gggatggatt ttgggtattt ggcgggtcat catggtcgag gcgattctat aaagcggttc
     9901 ctgtaactag aaacctttgt gaacaggttt ttgttgcggc taacggatct ccttgggaat
     9961 ggaaagagaa aagaactgaa ttgcaaacaa ttgggtccta tacaagaagc tgcagatgtg
    10021 caatagatcc tgctgttgga aacgggaaag tcagggctga tattttatgg gactgggtgg
    10081 agaaatatag ccgatgataa ttttgttttg attgacacca tttgtgactg ggacatataa
    10141 tctataccct taattgatac taagtctcaa tagagtgatt gtagttgtga caatatgaag
    10201 aatcaaggga agtttttcaa agtggatatc gaagatcctt gtgtgaggtg tggtttcaat
    10261 tgcacatgtg caataagagc tgtgggggca tgtgtggaat ttaacggtag tttagttttg
    10321 gatgacgtta cattctccgt aggcaagggg gttttatctg ccttggtggg tcccaatgct
    10381 gggggaaaaa gtactttatt taacgccatc gctggaatcc aagatatagt ccatggttcg
    10441 atatttgtta atggcctgcc ccccaaagat tcaaaaggtt ctattagcta tatacctcaa
    10501 cgagaaaatg ttaattggag attttcgttg acagccctag aagtggttga cctaggcctt
    10561 gtaaaaaata attctctttt agcattttca agaaagggga gcctggagaa ggttcgatct
    10621 gctttggaac gggtagatat gtggaattac aaggaccaat taattagcca aatgtcagga
    10681 ggacagcggc aaagagtttt tatcgccaga gctttatgcc aggaagcgaa tatattactt
    10741 ttagacgagg ctttcagtgg ggtggatgtg gcatctcaat tcgggttgat cgatatgctt
    10801 agagaactaa aagagcaagg gaaaactata ataattgcca ctcatgatct taatacccta
    10861 gctgatcgat tcgacgaggt catatgcctt aataggcacg tatgcgctca aggatctccg
    10921 gacacagcat ttactcctga ggtcttggaa gaattatatg gatcccaccc tgggatgttt
    10981 ttgagtcatt cgttaggtca tcaccatgat taacatcgtc cttgaaccgt tccaatatga
    11041 ctttatgctg cgagctttat ttgtatcaag catggttggc attatatgcc cgattttagg
    11101 ggcctatgta gttattagag gaatgggttt tatgggtgac gctatggctc acgcggttat
    11161 gccgggtata gtgattgctc taattctcgg tctctcccct ttcttgggtt ctgtgcctat
    11221 ggcgattgtt gttgcagtat cagtcggtta tttaattcac aagaaagatg tcagtgtgga
    11281 tacagccgtc ggggttatgt ttgcaggatt gttctctttg gggctagttt tgatgtcttt
    11341 agttggtggt cttgctataa gtattgagga tatcttactt ggacagattt tgggggtgtc
    11401 tagggttgat atgttgattt catttatttt gactgttctt gttctagtcg ctcttgttac
    11461 attccacaag caacttattt tttctgggtt tgatcccgta gggtctgtcg tagcaggttt
    11521 aaatgctggt gccttaaatt atatgttcct aattttgtta ggcatatcca ttgttgttac
    11581 tttgcacgta gttggagtgg ttttggttgt cgggttgttg ataactcctg ctgctgcgtc
    11641 tcagatggtg atgcgaagat tcacgagagc catgctttta ggaatagtct ttgggttgtt
    11701 ttctacagtg gttgggttat acctatccta ttatttcgat cttccttcag gacctgtaat
    11761 gactttgatt tcgtttgcga tttttctatg ctgtgccgtt tatgaaaaat ttttctctca
    11821 aacacagact tcgcatttga tgtagaactc tatgttatga ccgacaatcg aaccgggtat
    11881 gtctgattcg atgcctttca ataatttttc aacagtcgag actttaaatt ctccgacctt
    11941 tccacatgat acgcagacgg tatgatgatg gtgatctacc tgagagagtg aatatctagg
    12001 atttccgcct ggaatagaaa gacggcatat aacgtttgct tccaacagaa tcttcaaagt
    12061 tcgaaatacg gtagctcggg atacatttga tagatcatct gccatttgct cagcggtgaa
    12121 ccattcggcg gcagcatgga tgtaatctac tattctctgt cttggggccg taattctaag
    12181 tccatgatct tccaatagcg ccaatagatt cggcctagtc gtgtttgaat tagagtttgt
    12241 cattacagat tctcacccag acacagaata ccagatttat tagtctcggg aacattggaa
    12301 ctttgctcat tgcaacatcg ttctaagttt ttagattccg acaatgttaa acttccattc
    12361 caaatacaca gatatttact attgaaataa tgacaattga cctctagaaa agaatacagc
    12421 ccgactgact cagatcagac cacaaatact tcgatgaatc ctggacagaa cagatttaag
    12481 tttttctatg gttggtggat ttgcattggt ggggcttttg ttatggccat gtcatctgga
    12541 attaattttc atggatttgg caatttcatt attcctttga gtaaagaatt tggttggagt
    12601 agaaccaccg tgtcggctgt gttctccctg gctaggcttg aagctggatt tatcggacct
    12661 gtagaagggt gggcggttga tagactgggc ccaagaaaat tgatgttagt cggaatacct
    12721 gtgatgggtt tgggtttcat attattaagt caggttagtg gcctttttac attcatgttg
    12781 gtttatattc ttggcataac attaggtaac agtcttggaa tgcacgtgcc tgcctcagct
    12841 gctgttgcaa attggttcaa taggaaaaga gggttggcat ttgggatcat gtggtccggc
    12901 gtgggactag gagggttgtt ggtgccagcc ttggggtggg ctattgatgt ctacggttgg
    12961 agagatgctt cggtttatgt gggcattttc gttatatttg ttggtgttcc tgtcgctgct
    13021 ttgatgaggc acaggcctga gcaatacgga ttcttgcccg acggtcaaat aattcctcag
    13081 aaaacctcca atgggaaact cggcaagaac gaagatttag aagttgactt cacggctcga
    13141 gaggcgcttc agacttcgag tttctggttt ctatctttat cgataatggc gaggtctcta
    13201 gttactggag gggtcggact tcatctggtt ccgtactttg ttggattggg agccagcccc
    13261 atagaggctg cagcctatgc agggtcggtt ggcgtcatta gtattccagg acgcttcggt
    13321 ctcagttact taggtgatta ttttaatagg cgttatatga tggctatttc gctactattt
    13381 atgaccttat ctattgtgtt actagctaca gccgactccc tgtcttcatc cattcccgga
    13441 ttggttgcct attcaatctc tcaaggggga atttctgtca taccgcagtc cattattgcc
    13501 gactattttg ggaggaaggc attcgccaca atttctg
//
LOCUS       DASCNS010000291         5395 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002098402, whole genome shotgun sequence.
ACCESSION   DASCNS010000291 DASCNS010000000
VERSION     DASCNS010000291.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002098402"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /gene="argC"
                     /locus_tag="QF838_10240"
     CDS             complement(<1..324)
                     /gene="argC"
                     /locus_tag="QF838_10240"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF013298.3"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HJO61096.1"
                     /translation="MKVGIINVTGYAGAEVARILSEHEEVEIACVTGRSGAGKNISDI
                     FPHLANLDIEISPTLDKSVDFVFSARPHAASAEALKDAINSGIKGVDISADFRIRNLD
                     TYSEWY"
     gene            complement(395..1081)
                     /gene="nth"
                     /locus_tag="QF838_10245"
     CDS             complement(395..1081)
                     /gene="nth"
                     /locus_tag="QF838_10245"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HJO61097.1"
                     /translation="MTVIRNGPKTKKLKVKAVSDLLSIIYGPIPWKPRYNASEELVYT
                     ILSQHTSDINSERAFHNLLTVFGDLDHIADASVDDIEVAIRRGGLSKTKAPRIKSILN
                     EIREELGSFDLSFLAEMPLKDAKAWLINLKGVGPKTAAIILCFSFGMPAMPVDTHIYR
                     VSQRLSLVGKKISAEKAHDILESLVEPKEVFQFHMYLIKHGRDTCKAQRPKCHQCSLT
                     ELCPSFKRYQ"
     gene            1153..2154
                     /locus_tag="QF838_10250"
     CDS             1153..2154
                     /locus_tag="QF838_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013663.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HJO61098.1"
                     /translation="MTTVGIIANPSAGKDIRRLISQSRFVSNQEKVNTLRRVIKGLSG
                     VGVNEILLMPDYGRIAQEAVSNLQESISVKFLEMSIFNSEQDTITAAKLMESHGASAI
                     VTLGGDGTNRAVALGTCEVPIMPLSTGTNNVFPFLIEGTLAGMAAGTVAGHLVDRELC
                     APRCKKMNVYKSRDMAMDLIDTALVDIAISTETYVGARAIWDINTVSEIFLAVAKPDS
                     IGLSSIGGLTNPLSETDSHGLHIEIEKQIDSKYVSAPLTAGNIAKVGIKNISNMIPNH
                     PYVVKRSPCIIGLDGERSIPLRNGNSITIVLTGDGPPVIDPHKTLKLVGQNKIYFNE"
     gene            complement(2158..2676)
                     /locus_tag="QF838_10255"
     CDS             complement(2158..2676)
                     /locus_tag="QF838_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJO61099.1"
                     /translation="MSLNWENDPDLSSKSDMDYLQDLIESLTSLLHSTPEDKVLLFRR
                     GNAYLDKGKYEEAILDYSAIIGMNPSDSIPLNNRGICLRSLGDPDNAIRDFNKAIELN
                     NEYRDAYNNRGMELSDKEDYLNAIDDFTRAIEIDSEYWYAYNNRGMALWAIGKKDSAI
                     VDYNKVRSLIGS"
     gene            complement(2654..3175)
                     /locus_tag="QF838_10260"
     CDS             complement(2654..3175)
                     /locus_tag="QF838_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HJO61100.1"
                     /translation="MTVSEETLNHQVDCRGMPLIKSPDEIGVSASGIIYDRFGRVLLQ
                     KRTDIGWWGLPGGRLEIGENLTDCVEREVLEETNITVRAKALIKLYSDLTEYTAIQYP
                     NGKLVQYVTALFLCEKTSGYLIMSNESTDIGYYDPQHFPENTLLSTRLRVKDAREFSR
                     SDKGYFDVIELGK"
     gene            complement(3172..3786)
                     /locus_tag="QF838_10265"
     CDS             complement(3172..3786)
                     /locus_tag="QF838_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO61101.1"
                     /translation="MAISKWFFAQWYDVLNSIVEPKVVPYRNLTAGKSHGRTLEVGGG
                     TGANLPYFETTSSLTIVEPDVHMTNKLRKRVNNFNKDISIVKSFGEKLPFEDNIFDSV
                     VTTLVLCMVNDVEKVILEIYRVLKPGGTFFFYEHTVARSFSGRLLQQLLDPIWIITTT
                     GCHLKRDILGVVKAAPFTKLEYDIFNLNVGIPITIPNIVGSGTK"
     gene            complement(3799..4896)
                     /locus_tag="QF838_10270"
     CDS             complement(3799..4896)
                     /locus_tag="QF838_10270"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HJO61102.1"
                     /translation="MQSYRPNLGATIDLIETPSLLIDLNLMESNFDVISKTYSNTLCK
                     MRQHAKNIKCPDILWRQIEIGGTLNGVCTAKVSEAEVMVEGGIRDVLITSEIVGEDKQ
                     RRLAYLAKRADVKVCIDNKANLNSLSLVAQEIGSEIGVLIEVDTSMGRAGIRTVEQGV
                     DLAKLAESLNSIKFLGVMSHQTVDGFADKETRFIEGKRYIDKCLAVKTAIEDNGIEVT
                     IVSSGETFSYDVAPQIEGVTEVEGGTYALMSGPTKYMEEFSLAGKVLTTVIDVTEIEV
                     VIDAGLRCLGGPLGVTPTIEGHDNLAVKEMHDSYSVLKVEGVNPFEIGQTLFICTSQQ
                     DILVNRWDNFIGVRDGVVEAVWDISARGCHH"
     gene            complement(4912..>5395)
                     /locus_tag="QF838_10275"
     CDS             complement(4912..>5395)
                     /locus_tag="QF838_10275"
                     /inference="COORDINATES: protein motif:HMM:NF025397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61103.1"
                     /translation="LSAKSFLQSHSFTSNELLINTSNQSLKKAHANLPSEITGLISGN
                     YPFEINGYFPVCSVVSTVMSTPEPGRAYIDCGQKAISIDRGVPSVVNYPNTVVEKMSA
                     EHGYLVFDTAEMNLEIGDKILLRPANYSDTFNLYDFINLIKNDRLESVIETTARGTFK
                     "
BASE COUNT         1512 a         1143 c         1066 g         1674 t
ORIGIN      
        1 ataccattcc gaataagtgt cgagattccg aatgcggaag tccgcgctga tatctacgcc
       61 ttttattcct gaattgattg cgtcctttaa agcctctgca ctggcagcat gaggtcgtgc
      121 agaaaacaca aaatctacac ttttgtccag agtcggtgat atttctatat ctaaattagc
      181 caaatgagga aatatgtcag aaatattttt accggcccca ctccgaccag ttacacatgc
      241 aatttcgacc tcttcgtgtt cacttaaaat ccgggcaact tccgctcccg cataacctgt
      301 gacgttgata attcctactt tcatagtaaa ccttcggtat atttttaata ttttgccagg
      361 aggtatattc tagtacatta gtggtatttg caccctattg ataccttttg aatgaaggac
      421 acaattcagt gagagaacat tgatggcatt tcggcctttg ggccttgcag gtatctcgtc
      481 catgcttaat taagtacata tggaattgga aaacttcttt aggttctact aacgattcta
      541 aaatgtcatg ggctttctca gccgatattt ttttccccac caaagacaac ctctgggaca
      601 ctctataaat gtgagtatcc acaggcatgg cgggcattcc gaatgaaaaa caaagaatta
      661 tggctgcagt tttcggtccc acccccttta gatttataag ccaagccttg gcatctttga
      721 ggggcatttc ggcgagaaag ctaagatcaa aggaacctaa ttcctctctg atttcattga
      781 gaatggattt tatccgtggg gcctttgttt ttgagagtcc gcctctcctt atggctactt
      841 caatatcatc aacgctggca tcggctatgt gatccaaatc gccgaataca gttaataggt
      901 tatgaaaagc cctttctgaa ttgatgtctg aggtgtgttg ggacaaaatg gtatagacca
      961 gttcttcgga ggcattgtat ctaggtttcc atgggatagg accataaata atggaaagca
     1021 aatcagaaac tgctttaact ttcagttttt ttgtttttgg gccatttcta attacagtca
     1081 tatcaacaaa ctatccttat gaaacactat aatcggcttc atagaaaagt aaagatgtta
     1141 ggcacttgtt aaatgacaac ggttggaata atagcgaatc cctcggctgg taaagacata
     1201 aggcgactta tctctcaaag cagattcgtt tcaaatcagg aaaaggtcaa tactctacga
     1261 agagtcatta aaggactttc tggggttggc gtaaatgaaa tattacttat gccggactat
     1321 gggcgtattg cacaagaagc tgtctctaat ttacaagaga gcatttcggt aaagtttctt
     1381 gaaatgagta ttttcaattc cgagcaagat accattactg cagcaaaatt gatggaaagt
     1441 cacggagctt ctgccatcgt taccttgggg ggtgacggta ccaatcgtgc cgtggcttta
     1501 ggtacatgcg aggttccgat tatgccattg tctaccggta ctaataatgt gtttccattc
     1561 ttgattgagg gaacattagc tggtatggcc gcaggaactg tggcaggtca cttagtcgat
     1621 agagagttat gtgcccctag gtgtaaaaaa atgaatgttt ataagagccg ggatatggcg
     1681 atggatctga ttgatacggc tttagtggac atagctatat caacagaaac ttatgttggt
     1741 gcacgggcta tttgggatat aaacacagtg tcagagatat ttttagctgt tgctaaacct
     1801 gacagtatag gactttcctc tattggaggt cttacgaacc cgttatccga aacggattcc
     1861 catggacttc acattgaaat agagaagcag attgattcca agtatgtttc tgctcctctg
     1921 actgcaggta atattgccaa agttggaatc aaaaatattt ctaacatgat cccaaaccat
     1981 ccatatgtgg tgaaaagatc tccatgcata ataggtctag atggcgagcg atcaatacct
     2041 ttacgaaatg gaaattcaat cacgatagtt ttaaccgggg acggacctcc agtaatagac
     2101 cctcacaaaa ctctaaaatt agtcggccaa aacaagatat attttaacga atagtattta
     2161 agaaccgatt aacgatctta ctttgttgta gtcaacgatg gcagaatcct ttttcccgat
     2221 agcccacaac gccatgccac ggttgttata tgcataccaa tattcactat caatttctat
     2281 ggcccgagtg aaatcatcga tagcgttgag gtaatcctct ttatctgaga gttccatccc
     2341 acggttattg taggcatccc tatattcgtt gtttaattcg attgctttgt tgaagtccct
     2401 gattgcatta tcagggtcac caagggatcg taggcaaatt cccctattgt tcaatggaat
     2461 agaatctgat gggttcattc ctattattgc tgaataatcc aatatagctt cctcatattt
     2521 tcctttatct aggtatgcgt tgcccctgcg gaacaataga actttatctt ctggagttga
     2581 atgtaaaaga gaggtgagcg attcgatcaa atcctgtagg tagtccatat cacttttgga
     2641 acttaagtcc ggatcatttt cccaattcaa tgacatcaaa atatccttta tctgacctag
     2701 agaattctcg tgcatctttt acccttagtc tggtagagag taaagtgttt tccgggaaat
     2761 gttgagggtc gtaatatcct atatcagtgc tttcatttga cataatcaaa tatcctgaag
     2821 ttttttcaca aagaaataat gcagtcacgt attggactag ttttccgttt ggatattgga
     2881 tagcagtgta ttctgtaaga tcggaataca attttatcaa tgcttttgcc ctcactgtga
     2941 tgttagtttc ttcgaggacc tctctttcca cacagtcggt caaattctct ccaatttcta
     3001 aacggcctcc tggtaacccc caccaaccta tgtcagttcg tttttgaagg agtacgcgcc
     3061 caaatcggtc ataaatgatt cctgatgctg aaaccccgat ttcatcaggg ctttttatca
     3121 acggcatacc acgacaatca acttgatggt taagagtctc ttcagatact gtcatttggt
     3181 tcctgaaccc acaatgttgg gaatcgtaat tggaattccc acgttgagat tgaatatgtc
     3241 atattctaat ttggtaaacg gagccgcttt tacaacacct agtatatctc ttttaaggtg
     3301 gcatcccgtg gtggtaataa tccatatagg gtctaaaagt tgctggagta atctcccgga
     3361 aaaactacga gcaacagtat gttcgtagaa gaaaaatgtc ccacccggtt taagtactcg
     3421 ataaatctcc aagataactt tttcaacatc atttaccata caaagaacca gggttgtgac
     3481 aaccgaatca aagatgttgt cctcgaatgg gagtttttcc ccaaatgatt tcacgatgga
     3541 tatatctttg ttgaagttat taaccctctt gcgaagctta ttggtcatgt gaacatcggg
     3601 ttcgactatg gttaaggagc tggtcgtttc gaaatatggc aaatttgctc ccgttccccc
     3661 accgacttcc agggttctgc catggctttt cccagctgtt aaatttcgat acggaacgac
     3721 tttgggttca acaatcgaat ttaagacatc gtaccattga gcgaagaacc attttgatat
     3781 agccattatt taactaactt agtgatggca tcctctggct gatatgtccc aaacagcttc
     3841 taccacgcca tcacgtactc caatgaaatt atcccatcga ttaaccaaaa tatcttgctg
     3901 ggaagtgcag ataaacaaag tttgaccgat ttcgaaagga tttacccctt ctactttgag
     3961 aacactatag gaatcatgca tttctttaac ggctaaatta tcgtgtcctt caatggttgg
     4021 tgttacccct agaggtccgc ccaaacaccg tagtccagcg tcgatcacaa cctcaatttc
     4081 cgtcacgtct ataacggtcg ttaaaacctt tcctgctaga gaaaattctt ccatatattt
     4141 tgtgggcccg ctcatgagag cataggtgcc cccctctact tcagtcacac cttctatttg
     4201 aggggcaacg tcataagaaa aagtttcccc agacgaaacg attgtaactt caatcccatt
     4261 atcttctatg gcagtcttca ctgccaggca tttgtctata taccgtttac cctctataaa
     4321 tcgcgtttcc ttatccgcga aaccatcgac tgtttgatgg ctcataacac ccaaaaattt
     4381 tattgaattt agcgattcag ccaattttgc taggtcgact ccctgttcaa cagttcgaat
     4441 gcctgcccgc cccatggagg tatcaacttc tatcaagacc ccaatttcag aacctatttc
     4501 ctgagctact aaagacaaag aatttaaatt tgctttattg tcaatgcaaa ctttcacatc
     4561 cgctcttttt gccagatatg ccaaccttct ttgtttatct tcacctacaa tttcactggt
     4621 gattagaaca tcccgtatgc cgccttcaac catgacttca gcctctgaaa ccttggctgt
     4681 gcacaccccg tttaaagtgc caccaatttc aatttggcgc cataatatgt cgggacactt
     4741 tatgtttttt gcatgctgac gcattttaca tagggtattg gaataagttt ttgaaataac
     4801 atcaaaattc gattccatta aattgagatc tataagaaga cttggggttt ctatcagatc
     4861 aattgtggcc cctaggtttg gccggtatga ttgcataatg ttttcctgat tttatttgaa
     4921 tgtgccacgg gcggtggttt cgatgactga ttctaatcga tcatttttta tgaggtttat
     4981 aaaatcgtaa aggttaaagg tgtctgagta gttagctggc ctcagtaaaa ttttgtcacc
     5041 gatttccaag ttcatctcag ctgtgtcgaa aacaaggtac ccgtgttcgg cgctcatttt
     5101 ttctaccaca gtatttggat aattgacgac ggatggaact cctcgatcta tgctaatagc
     5161 tttttgtcca caatctatgt atgcccgccc gggttccggg gtactcataa ctgtcgacac
     5221 taccgagcag actgggaaat acccatttat ctcgaaagga taatttccag atatcaaacc
     5281 agttatctcc gacggcagat tggcatgagc tttcttcaaa gattgatttg atgtgtttat
     5341 taacaactca tttgaggtga aggaatgaga ttgaagaaaa gatttggcag ataga
//
LOCUS       DASCNS010000292         1716 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002101576, whole genome shotgun sequence.
ACCESSION   DASCNS010000292 DASCNS010000000
VERSION     DASCNS010000292.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002101576"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="QF838_10280"
     CDS             <1..1599
                     /locus_tag="QF838_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013225.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HJO61104.1"
                     /translation="SMSARDRVNSIVDFGSFKEIDRNLVSVDPISFTSSTSYKETLHK
                     DQKRTGLTEAVLSGTCSIDGILCMIISFDFGFMGGSMGCAVGQKISNAIETAEKKKLP
                     LLTIISGGGSRIQEGVLSLMQMAKTTIAVNRHNAKGLPFISIFSNPSTGQAFASFANL
                     ADITIAEPGAILGYNPIGTTRTENSSTPFDRQTSESHLEHGLIDSIASRTELREVVSV
                     ILDLLQKEFTLNRTNRRGEISVDTPKNLAWHSVQLARHSSRPTSLDYMARMLDNFVEI
                     HGDKQYSDDPAISCGIGQLGGQTIVVIGQNRSKDQVSGNVLRILPEGFRKCQRAIKIA
                     RKFNLPIVSLIDTPGADTSIEAENRGLAHSIATTMSLFADQKAPTIAVVIGEGGSGGA
                     LSLGIADRVIMLENAIFSAISPEEAANLIYRDENRANEAAETLKLTAADCKSLGIVDV
                     IVSEPGGGAHTDHHESARLLKRALLQQLFEVQNNSARKRLRERYKKFKDIGEYSSYFR
                     STVTRELNLLQHSITERIKGLRRA"
BASE COUNT          550 a          348 c          360 g          458 t
ORIGIN      
        1 agcatgagtg ccagagacag agttaattct attgttgatt ttggatcatt taaagaaatt
       61 gacaggaatt tggtatctgt tgaccctatt tcttttacat cttcaacttc ttacaaagaa
      121 actctccata aggatcaaaa gcgaactgga ctcacagagg ctgttttgag tggcacctgc
      181 agtattgatg gcattctttg tatgatcatc tcatttgact ttggatttat gggagggtct
      241 atggggtgtg cagtcggaca gaagatatcc aatgccatcg aaacagctga aaagaagaaa
      301 ttgcctctac taaccatcat atctggaggt ggatctcgaa tccaagaagg ggttttgtca
      361 ttgatgcaga tggccaaaac cacaatcgca gtcaataggc ataacgccaa aggactcccg
      421 tttatatcta ttttttcaaa tccatctaca ggacaggctt tcgccagttt tgccaatcta
      481 gccgatataa cgatcgctga acctggagcc attctaggat ataaccctat aggtacaact
      541 cgaactgaaa acagttcaac cccctttgac cgacaaacgt ctgagtcaca tctcgaacac
      601 gggttgattg attccattgc ctcccgaaca gaattaagag aagtggtttc tgtcatatta
      661 gatctacttc aaaaagaatt tactttaaac aggacaaata gaagaggtga aatttctgtt
      721 gacactccaa aaaatttagc ctggcattct gttcaattag ctcgtcattc atctcggccc
      781 acatctttgg attatatggc acgtatgttg gataattttg tggagataca tggggataaa
      841 caatattctg atgacccggc aataagctgt ggaatcggac aattgggagg ccaaaccatt
      901 gttgttattg gacagaaccg ttcaaaagac caagtatcag gaaatgtatt aagaatattg
      961 ccagaagggt ttcggaaatg ccaaagagct ataaaaattg ccaggaaatt caatttaccg
     1021 atagtatcat taatagatac tcctggagca gatacttcca tcgaagccga aaatcgggga
     1081 ctcgctcatt caatcgcaac gacaatgtct ctctttgctg atcaaaaagc acctactatt
     1141 gcagttgtga ttggagaagg aggaagcgga ggggctcttt cattaggaat agcggacaga
     1201 gtaatcatgc tggaaaatgc tattttttca gccatatccc cggaggaagc cgcaaatcta
     1261 atatatagag atgaaaacag ggctaatgaa gcagctgaaa cactgaaact cacggctgca
     1321 gattgtaaat ccctaggaat agtcgatgtg attgtttcag aacctggcgg cggtgcacat
     1381 accgatcatc atgaatcagc cagactgctc aaaagagcat tgttacaaca actgtttgaa
     1441 gttcaaaaca actcagccag aaaaaggttg agagaacgct acaaaaagtt taaagatatt
     1501 ggtgaatata gttcatattt ccgaagtact gtaaccagag aattgaacct tctccaacat
     1561 tcaatcacag agcgaattaa aggccttaga cgggcctagg aatttgttaa cataatgagt
     1621 ctagaaactt aaaaccccac cctccttcga acgcccagac tcaaagacag atatatctgt
     1681 ctcgtcttct aaggtgaggc ctatgtcatc tagtcc
//
LOCUS       DASCNS010000293        10437 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002105940, whole genome shotgun sequence.
ACCESSION   DASCNS010000293 DASCNS010000000
VERSION     DASCNS010000293.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002105940"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="QF838_10285"
     CDS             <1..862
                     /locus_tag="QF838_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJO61105.1"
                     /translation="HVLGADVSGEVVATGPKVTKVSEGDRVVVNPRITCMQCRYCQSN
                     RSEYCSSAGMVGSAIRGGYAEYVVVPATNAFPIPDDLSYVQAASLPTVFMPSWNILIR
                     RSQLKPWETALVPSASSGVGTAAIQVAKNIAKSSVISTTSTIEKAEKATALGSDHVIN
                     YKTEDVSERLKEITEGRGVDVVIDHVGTDVWNAANRRLAKGARYGICGVTSGYKAELQ
                     MGAMFTGHLTMFGVFMGRSEDLEQIIYHAGQGTIKGVVDSEYSLQDVRKAHEAMEGLD
                     FFGKLVITVD"
     gene            complement(923..1183)
                     /locus_tag="QF838_10290"
     CDS             complement(923..1183)
                     /locus_tag="QF838_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014841.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61106.1"
                     /translation="MKTNEFAGAGLDVFDIEPTPTDNPLFQMENVMVTPHSAGTSNRA
                     VIAAQIQVGQEAARLLNGSWPMSLVNPEVRSSIDVRSLATRG"
     gene            complement(1170..1940)
                     /locus_tag="QF838_10295"
     CDS             complement(1170..1940)
                     /locus_tag="QF838_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61107.1"
                     /translation="MSKLKVAMISRSNPETSLVQQQLEGIDCEFDVYVCQSEGETIEA
                     VKGVDVIINQGVPMPSTVMDEIDSAQGIVSFGHGFNHIDHESATDNSVMVINTAGFCT
                     EEVSNHAIMFILSCAKKLTILNDLTKEGKWGTETGSVLSNMVPVTDQTLGIVSLGNIG
                     RAVARKAQALGMDVIAYDPYVQPWVAKEYRVELVSSLKELASRSDFVSIHTPLNNQTR
                     HLVGSDFFKAMKPTAYFINTLQGRYGRRGCSNQCLENQ"
     gene            complement(2050..3513)
                     /locus_tag="QF838_10300"
     CDS             complement(2050..3513)
                     /locus_tag="QF838_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HJO61108.1"
                     /translation="MGKATGFKEFGRSTPKRQPVGDRLQNFHEFYTEWNDDDAQAQGS
                     RCMNCAVPFCMSGCPLGNLIPDFNDSVYKKQWEGALKELHSTNNFPEFTGRICPAPCE
                     ASCVLNINQDPVTIEYIEKAISDKGWQEGWIKPEPPKVRSGKKVAIVGSGPAGLAAAQ
                     QLNRTGHHVTVYERSHRTGGLLALGIPDFKLEKYVIDRRIDQMEKEGVIFFTDIEIGT
                     DVPASTLADDFDVVLLTGGSTIPRDLDIPGRELKGVHFAMEFLTQQNNVNAGFVVEEK
                     DRIVTTEKNVVILGGGDTGSDCLGTSHRQGAGQVHQLELLPEPPKERRENDRWPNWPM
                     ILRTSSSHEEGGFRDYNILTKKFTGENGVLKKLHGVRLEWGKPDENGRPQMMEVEGSE
                     FEIETDYVFLALGFLHPQHEGPVKELKLDLDPRGNVKTDSNKMTNVPGIFAAGDISRG
                     QSLVVWALAEGREAARGIDEYLMGETSLPKSAGSYIG"
     gene            complement(3506..8062)
                     /gene="gltB"
                     /locus_tag="QF838_10305"
     CDS             complement(3506..8062)
                     /gene="gltB"
                     /locus_tag="QF838_10305"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HJO61109.1"
                     /translation="MKSPYENKKLRKLVKQGLYDPVFEHDACGVGMVANIKGKQSHSI
                     VSQGLEALSHLAHRGAAGADPETGDGAGILIQMPHDFFKLELSSTGCDLPDPGQYGVG
                     MAFLPSDKGEAELSRSLIEECVLKQGYDFLAWRQVPIDPEKIGVLANRDRPHITQFFV
                     TPKGNVPKNIDRDLYLLRRAIESSFDSASLENADDFYICSLSSSTIVYKGLVMAEQLE
                     DFYKDLTDANLVSSFALVHSRFSTNTLGSWKLAHPYRFIIHNGEINTVRGNTNWMLAR
                     EQVFESSDPDVNTSDLVPVIKPGQSDTAALDNALELLLATGRSIEHSMMMLIPEAWGD
                     HIPMEQSKKDFYEYHSSMMEAWDGPALVIGTDGTKVCAVLDRNGLRPCRYLVTTDDVL
                     VMASETGVIDVPPEKVLYKERIYPGRMFLLDTKKGELVNDLDLKAGLAGSKPYGEWLS
                     QNRIFLEDLPEAVNATYEKIIDIKKQQNTFGYTLEDFRMILEPMAVNGYEPTGSMGND
                     APLAFLSDHNPILFNYFKQLFAQVSNPPLDAIREELVTSLRGTLGEEKNLFAETPDHC
                     RQITLRQPIITDLDLGRIKNSSQKNLTAKTISTLFDPSVTSGFKDALSRINKEASDAI
                     KEGHSILILSDRGVGKSQAPLPSLLATSAVHHHLIRKGERSKAGIVVETGEAREVAHF
                     AALIGYGAAAINPYLAFETIRNQISQKEFENDIDATEAIRNYISACHKGILKIMSKMG
                     ISTIQSYRGAQIFEAVGLNQSVIDEYFTWTPSRIGGIGIEEIEREYITRHTSGYGTKR
                     NAANLEVSQGGQYQWRRDGEFHMWNPNTITSLQHAVRENSWDSYEQFADSVDQETRRL
                     STIRGLLDFKKSDNPISIDEVEPAKEIVRRFATGAISLGSISREAHETMAIAMNRIQA
                     RSNTGEGGEDHSRYVLDPNGDSRNSAIKQVASGRFGVTINYLANAKDIQIKMAQGSKP
                     GEGGQLPGHKVDEYIGGVRNSTPGVELISPPPHHDIYSIEDLAQLIHDLKNANPEARI
                     HVKLVAEAGVGTIAAGVSKGHGDVVLISGDTGGTGASPESSIKHAGLPWELGVAETQQ
                     VLVMNDLRGRIVVQADGQIKTGRDVAIACLLGAEEFGLATGPLIAMGCIMLRKCHLNT
                     CSVGIATQDPELREKFAGKPEHVINYFFFIAEQLRKIMADLGIKKIEDMVGRTDLLDK
                     RDLIAHWKTEGVDFSQLLHMPDVPEGVAKHKIMEQDHSLEKALDNELIKDATVSIDTG
                     KPITITKEIDNSYRTVGTTLSHTIAKKYGEDGLPENTISLDFSGSGGQSFAAFLSKGV
                     SIDLKGDSNDYFCKGLSGGKVVIRPHEKSTFTPEENIIIGNVALYGATGGQVFIRGIA
                     GERFAVRNSGAEAVVEGVGDHGCEYMTRGKVVILGPTGRNFAAGMSGGEAYVFDEEGK
                     FESLCNKGLVTLEKISEEKDLKDLKRLIELHKKHTGSKNAERIMSDWSSMTRKFVKVM
                     PVDYKRVLAERQARQADKVVSANG"
     gene            complement(8094..9431)
                     /locus_tag="QF838_10310"
     CDS             complement(8094..9431)
                     /locus_tag="QF838_10310"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HJO61110.1"
                     /translation="MLERNSEAVEYVLREARENDVKFIRLWFSDILGNIKGFAITVVE
                     LENALTRGMGFDGSAIEGYARADERDLYALPDPTTFSLLPWRPKTNAVAKMFCDIITP
                     DDKEFEGDPRSVLKKNLNRASEKGYTYYVSPELEYFYFKDSSSTVGLDNSGYFDQNSP
                     DVGTDLRRDTVLTLEELGIPVESSHHESAPSQHEIDLRHTDALTMADSVMTYRLVVKE
                     IAMAKGYYASFMPKPIADINGNGMHTHMSLFKGDENVFYGEKNEAHLSDIAKHFCAGL
                     MKHAREITLVTNQWVNSYKRLVPAYEAPTYITWAEVNRSDLIRIPSFKPDRKSSRRIE
                     YRAPDPSCNPYLAFSVMLAAGLEGIDNKYEMPASLTKSVHEMSENERTSLGIESLPGN
                     LWEAIQITEDSELVKKTLGDSVFNSFIENKKIEWNRYSSQISPYEVERYLPIL"
     gene            complement(9618..>10437)
                     /locus_tag="QF838_10315"
     CDS             complement(9618..>10437)
                     /locus_tag="QF838_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61111.1"
                     /translation="VNDFGLRIEAQHHEVATGGQAEIDMRFGPLLQTADNVMMYKYAV
                     RNVAKQHGKVVTFMPKPLFDDNGTGMHTHQSIWKNGEPLFAGNEYAGLSQLALNYIGG
                     LIKHGKALMALAAPTTNSYKRLTPGFEAPTILALSARNRSAACRIPMYFDNPAAKRVE
                     FRSADPTCNPYLTFAAMLMAGIDGIENGYSPGEPLQQDLFELSAEETATFPQVPGSLE
                     EALKALQDDHEFLLKGGVFTEDVIENWISFKTENDVDPVRLRPHPYEFHLSFDA"
BASE COUNT         2669 a         2408 c         2092 g         3268 t
ORIGIN      
        1 ccatgttttg ggtgcagatg tgtctggaga agttgtcgcg actggaccca aagtaaccaa
       61 ggtatctgaa ggggacagag tcgttgtgaa tccaagaata acttgtatgc agtgccgata
      121 ttgtcaatcg aatcgaagcg aatattgctc ttcagcaggg atggttggat cagccattag
      181 aggaggatat gcagaatatg tagtagtgcc agcgactaac gcctttccca ttccagatga
      241 tttaagttac gtacaggcag cctccttacc aactgtattt atgccaagtt ggaatatact
      301 tattagaaga tctcagctta agccctggga aacggcacta gttccctcag cctctagtgg
      361 tgtcggcacc gcagctatcc aagttgcaaa aaacatagcg aaatctagcg ttatatctac
      421 tactagtaca attgaaaaag ctgaaaaagc cactgctctt ggatccgacc atgtcatcaa
      481 ctataaaact gaagatgtct ctgagcgttt gaaggaaata accgaaggac gaggtgttga
      541 tgtggtaata gaccatgtgg gaacggatgt ttggaatgcc gctaataggc gtcttgctaa
      601 aggcgcgaga tacggaattt gcggagttac atcagggtac aaggctgagc tacagatggg
      661 tgccatgttc acgggtcatt tgaccatgtt tggagtgttt atgggccgta gtgaagattt
      721 ggaacaaatt atctaccatg ccggccaggg gacaataaag ggtgtagttg attcagaata
      781 ttccttgcaa gatgttagaa aggcacacga agccatggag ggcctagatt ttttcgggaa
      841 actagtaatt acagtagatt gatttaactt acggaccttt aaaggctgag atcgccagtt
      901 ggaatctgac ctggtctcct tcttaacctc tagttgccag agaccgaacg tcgatgctgg
      961 atctaacctc aggattcacc aaagacatag gccaagaccc atttaataac ctagcagctt
     1021 cctgcccgac ttgtatttga gccgctatga cagccctatt ggaagtccct gctgagtgag
     1081 gggtcaccat gacattttcc atttgaaata agggattgtc agttggagta ggctcaatat
     1141 caaaaacatc taatcctgcc cctgcaaact cattggtttt caaggcattg attagagcat
     1201 cctcgtctac cgtacctccc ctgcaaagta ttaatgaaat aggcggttgg cttcatagct
     1261 ttgaaaaaat ctgatccgac tagatgtctc gtttgattgt tcagaggagt gtgtatggaa
     1321 acaaaatcag atcgtgaggc gagttctttt agagaactaa ccaactcgac gcggtattcc
     1381 tttgctaccc aaggttgaac atatgggtcg tatgcaatga catccattcc tagtgcttgt
     1441 gcttttctgg caacggctct tcctatgtta cctaaagaca ctataccaag tgtttggtct
     1501 gtgacgggca ccatattgga aaggacagag ccagtttcag taccccattt gccttccttt
     1561 gttaagtcat taagaatcgt tagctttttg gcacaagata atatgaacat tatcgcgtgg
     1621 tttgaaacct cttcagtaca aaaacctgcc gtattgatca ccataacgga attgtctgta
     1681 gcggactcat gatcaatatg attgaaaccg tgtccgaagc taacaatccc ttgagctgaa
     1741 tctatttcgt ccatcacggt gcttggcatg ggaacacctt ggtttattat tacgtccact
     1801 ccttttacag cttctatagt ttcaccttcg ctctggcaaa catatacatc gaattcgcaa
     1861 tcgattcctt ctagctgttg ttgaacgagg ctagtttcag gattgcttct ggatatcata
     1921 gccaccttaa gtttcgacat atcatctccc caattgaaat aatgtttttt tatgggctga
     1981 ttatagctat aaaattattg tgttgtgtaa aataagtgag agacctagct gaacggtaaa
     2041 caccgtattc taaccgatgt atgaaccagc agatttagga agactagtct ctcccattaa
     2101 atattcgtct atcccgcgtg ctgcttctct tccttcagct agtgcccata ccaccaagga
     2161 ttgacctcta gatatgtctc ctgcggcgaa tatacctggt acattagtca tcttattgga
     2221 gtcagttttt acatttccac gtggatccaa atctaacttt aattctttaa ctggtccctc
     2281 atgttgaggg tgcagaaaac ctaaagccaa gaagacgtag tccgtttcta tttcaaattc
     2341 gctaccttca acttccatca tttgaggtcg tccgttttcg tcaggttttc cccattccaa
     2401 cctgacgccg tggagttttt tcaaaactcc gttttcaccc gtgaatttct tcgtgagtat
     2461 gttgtagtcc ctgaaccctc cctcttcgtg agaagatgag gttctaagaa tcataggcca
     2521 gtttggccac ctgtcgtttt ctcgtcgttc ttttggcggt tctggaagga gttcaagctg
     2581 atgcacttgg ccggctcctt gccggtgaga cgttccaaga cagtctgacc cagtatctcc
     2641 accacccaag ataaccacat tcttttctgt tgtcacaatt cgatcttttt cttcaaccac
     2701 aaaacctgca ttaacattgt tctgttgagt gaggaattcc atcgcaaaat gaaccccttt
     2761 taattccctt ccagggatat ccagatccct tggtatcgta gatcctccgg tcaagagaac
     2821 tacgtcaaaa tcatcagcta gtgtggacgc tggcacatct gttccgattt caatgtcggt
     2881 aaaaaatatt accccttcct tttccatttg atcaattcta cgatcaataa cgtatttttc
     2941 gagtttaaaa tcagggatac ccaatgccaa taggccacca gttctgtgtg atctttcata
     3001 gacagtcaca tgatgtcctg tccggttaag ctgttgggct gctgcgagac cggccggtcc
     3061 agaccctact atggcgactt tttttcctga tcgtaccttg ggtggttcag gtttgatcca
     3121 gccttcttgc caccccttat cagatattgc tttttctatg tactctattg tgactgggtc
     3181 ctggtttata tttagtacac atgaagcttc gcacggggcg gggcatattc ttccagtgaa
     3241 ttcagggaaa ttattggttg aatgaagttc tttcaaggct ccttcccact gctttttata
     3301 aaccgaatca ttaaagtccg gaatcagatt tcccaaagga catccactca tacagaaagg
     3361 aactgcgcaa ttcatgcatc ggctgccttg cgcctgagca tcatcatcat tccattccgt
     3421 ataaaattca tggaaatttt gtagtctatc tcctacaggc tgtctcttgg gtgttgacct
     3481 tccaaattct ttaaatcctg ttgccttacc cattggcgct caccacctta tctgcctgcc
     3541 gggcctgtct ttcagccaaa acccgtttgt agtcaacagg catgactttt acaaatttcc
     3601 tagtcattga actccaatct gacataattc tttcagcatt tttgcttccg gtatgttttt
     3661 tatgcaattc aattaatctt ttcagatctt tcaaatcttt ttcttcagag attttctcta
     3721 aggtaactag gcctttgttg caaagagatt caaattttcc ctcttcgtcg aatacatagg
     3781 cttctccacc actcattccg gctgcaaaat tacgtcctgt tggcccaagt attacaactt
     3841 tacctcttgt catgtactcg cacccgtgat ctccaactcc ttcaacgaca gcctcggcac
     3901 cactgtttct taccgcaaat cgttcaccgg cgatgcctct aatgaaaact tgtcccccag
     3961 tcgcaccata caatgctacg ttaccaataa ttatgttctc ctcaggtgta aaagtggatt
     4021 tttcatgggg acgtatcact acctttccac cagacaaacc cttacaaaaa tagtcgttgg
     4081 aatcaccttt gaggtcaatt gacacccctt tacttaggaa ggcggcaaag ctttgccctc
     4141 cagaaccact aaaatctagg gaaatagtgt tttctggcaa tccatcttcc ccatattttt
     4201 ttgcaatcgt gtgactcaat gtggtcccta cggtccggta tgaattgtcg atttcctttg
     4261 tgatggttat tggttttcct gtatctattg aaacagttgc gtcctttatt aactcattat
     4321 ccaaagcttt ttctaatgag tggtcctgtt ccattatttt atgttttgcc actccttctg
     4381 ggacatccgg catgtgaagt aattgactaa aatccacacc ctctgttttc caatgtgcga
     4441 ttagatctct tttatcgagc agatccgttc gccctaccat gtcctcgatt tttttgattc
     4501 ctaggtcggc cattattttt ctcaattgtt cagctatgaa gaaaaaataa tttattacat
     4561 gttctggctt tccagcgaat ttctcccgca attcagggtc ttgtgtcgct atgcctactg
     4621 agcatgtgtt taaatggcat ttccttagca tgatgcaacc cattgcgatc aacggaccag
     4681 tagcaagccc aaattcttct gctcctagca aacaagcaat tgcgacgtct ctgccagttt
     4741 taatttgccc atcagcttgc accacaatac gacctctgag gtcattcatg accaagacct
     4801 gttgagtttc ggctacaccc aattcccacg gcagcccggc atgtttaata gaagattctg
     4861 gggatgcacc cgttcctcca gtgtcaccac ttatcagtac tacatcaccg tgccctttcg
     4921 aaacccctgc tgcaattgtg ccaacaccag cctctgcaac tagtttgaca tgaattcttg
     4981 cctctggatt agcattcttc agatcgtgta ttaactgagc taggtcttcg atagagtata
     5041 tgtcgtggtg cggtggggga gatataagct ctacacctgg agttgaattt ctcactcctc
     5101 cgatgtattc gtcaactttg tggccaggaa gctgccctcc ttctccaggc ttggaacctt
     5161 gagccatttt gatttgtata tcttttgcat tggccaggta attgatggta acgccaaatc
     5221 ttcctgaggc cacttgtttt attgcactgt ttcttgaatc cccattaggg tctaagacgt
     5281 acctagagtg atcctcacca ccttcccctg tattgcttct agcctgaatc cgattcatcg
     5341 ctattgccat ggtttcatga gcttctctac taattgagcc aagagatata gctcctgttg
     5401 cgaacctcct cacaatttcc ttagcaggct caacttcatc aatcgatatc ggattatcgg
     5461 acttcttaaa atcaaggagc cctctgattg tgcttagcct tctagtttct tgatcaacgg
     5521 aatccgcaaa ttgttcatag ctgtcccaac tgttttctct aacagcgtgt tgaagagatg
     5581 taattgtgtt tgggttccac atatgaaatt cgccatctct tcgccattga tattgcccgc
     5641 cctgcgaaac ttccaggtta gccgcatttc ttttagttcc ataaccggaa gtatgacggg
     5701 tgatatattc cctctctatt tcttcaattc caatgccgcc tattcgagat ggagtccaag
     5761 tgaagtattc atcaatgaca gattgattta aacctacggc ctcaaaaatt tgtgctcccc
     5821 tataactttg gatggttgag atacccattt ttgacattat cttcagaatg cccttgtgac
     5881 atgctgaaat gtaattgcga attgcctcgg tagcatctat gtcgttttca aactctttct
     5941 gtgaaatttg attccgaatg gtctcgaaag ctaaatatgg atttattgcc gcagccccgt
     6001 accctattag agcagcaaaa tgggctactt ctcgcgcttc acctgtctcc acaactattc
     6061 cggctttaga tctttcccct ttcctaatta aatgatgatg cacggccgaa gtcgctaaaa
     6121 gacttggcaa tggagcctgg gatttaccta cccccctatc tgataatatc agtatcgagt
     6181 gcccttcttt tatagcgtct gatgcttctt tatttattct gctcagcgca tctttgaaac
     6241 cactagtgac actggggtca aataatgtag atatagtttt tgctgtcagg tttttttggg
     6301 atgagttttt aatgcggccg agatctaaat ctgtaattat cggctgtctt aaagtgatct
     6361 gacggcaatg atctggagtc tcggcgaata ggtttttttc ctcacctaac gtgcctctta
     6421 aggaggttac caattcttcc ctgattgcat ccagaggggg attactgacc tgtgcgaaca
     6481 gttgcttgaa ataattgaac agtattggat tatggtcaga aagaaaggca agaggggcat
     6541 cgttacccat cgaacctgtt ggttcgtacc cgttcactgc catcggttcc aatatcattc
     6601 taaaatcttc taatgtgtat ccgaatgtgt tctgttgctt ctttatgtcg atgatttttt
     6661 cataggttgc atttaccgct tctggtaaat cttctagaaa tatcctgttt tgagaaagcc
     6721 attcgccgta tggtttggaa ccggctaaac ctgctttcag atcgagatcg ttgacgagct
     6781 ctcctttttt tgtgtccaat aaaaacatcc ggcctgggta gattctctct ttgtaaagaa
     6841 ctttctcggg agggacgtca atcactccag tttcagaggc cattaccagt acatcatcag
     6901 tggttactaa atagcggcaa ggtctaaggc cgttgcggtc taataccgcg cagactttcg
     6961 tcccatctgt ccctatcaca agggccgggc cgtcccaagc ttccatcatg cttgagtgat
     7021 attcatagaa atcctttttc gattgttcca tcggtatgtg atcaccccac gcttcgggta
     7081 tcaacatcat cattgaatgc tctatgcttc tgccggtcgc tagtaagagt tcaagcgcat
     7141 tgtcaagagc agcagtatca ctttgccctg gtttaataac gggaaccaaa tcacttgtgt
     7201 ttacatctgg atccgatgat tcgaaaacct gttctctagc caacatccaa ttggtgttgc
     7261 cacgcaccgt gtttatttca ccattgtgga ttatgaatct gtatggatgt gctagtttcc
     7321 aactccctaa agtgttggta ctaaacctgg aatgtacgag agcaaatgaa ctgacgagat
     7381 tggcatcagt caaatctttg taaaaatctt ctagctgttc agccattaca aggcccttat
     7441 aaactatagt actggaagac aatgagcaga tatagaagtc gtcagcattt tctaaagaag
     7501 ctgagtcaaa tgaagattca atagctcttc ttagtaagta caagtctctg tcaatgtttt
     7561 taggaacatt tcccttggga gtaacaaaaa attgagttat atgagggcgg tctcggtttg
     7621 ccaatacacc tattttttct gggtctattg gaacttgcct ccatgcaaga aagtcataac
     7681 cttgttttaa aacacattcc tcaatcaaag acctacttaa ttctgcttca cctttgtcag
     7741 atggtaaaaa tgccatgccc accccatatt ggcctggatc gggcaaatcg catcctgtgg
     7801 atgacagttc caatttaaaa aaatcatgag gcatttgtat gaggattcca gctccatccc
     7861 ctgtttcagg atcagcacct gcagcccctc tatgtgctaa atgagataaa gcctctaacc
     7921 cctgcgaaac tatggaatga gactgctttc cttttatgtt ggcaaccatc ccaactccac
     7981 aggcatcatg ctcaaataca ggatcataaa gcccttgttt aacgagtttt cttaatttct
     8041 tattttcata tggtgatttc atctgttttc caatgcattg cctttacctt tccttacaaa
     8101 atgggtaggt aacgctctac ttcatatggt gaaatttgtg aagaatacct attccattct
     8161 atttttttgt tttcaataaa actattaaat acactgtcgc ctaaggtttt tttgactagt
     8221 tcactgtctt cagttatctg gatagcttcc cataaatttc caggtagaga ctctattcca
     8281 agagaagttc tttcgttttc cgacatctca tgaacactct tagttaatga ggcgggcatc
     8341 tcatacttat tgtcgatgcc ttccaatcca gcggctaaca ttacactgaa cgccaaatag
     8401 gggttgcagg atgggtcggg cgctcgatac tcaatccttc tagaggattt cctatctggt
     8461 ttaaaagaag gaattcttat taaatctgac ctattgacct ccgcccacgt tatgtaagtc
     8521 ggcgcttcat atgctggaac caaccttttg taagaattga cccactggtt tgttactaga
     8581 gttatttctc tagcgtgttt cataagtcca gcgcaaaaat gttttgctat gtctgataag
     8641 tgagcctcat ttttctcacc atagaataca ttttcatccc ctttaaagag ggacatatgc
     8701 gtatgcattc cgttgccgtt aatgtccgct attggcttag gcatgaaaga ggcataatac
     8761 cccttggcca ttgctatttc ttttaccact aatctgtagg tcataacaga atcagccatt
     8821 gttaaagcat cagtatgtcg caagtctatt tcatgttggc ttggcgctga ttcgtgatgg
     8881 ctcgattcca ctgggattcc taattcttcc aaggttaaaa ctgtatctcg ccttaaatca
     8941 gtaccgacat caggggaatt ttgatcaaaa tacccactgt tatctaatcc tacggttgag
     9001 cttgaatcct tgaaataaaa atactcaagt tcaggagaga catagtaggt ataacctttt
     9061 tctgatgctc gattgaggtt ttttttgagg acggatcgag ggtcgccttc aaattctttg
     9121 tcatctgggg tgatgatgtc gcaaaacatt ttggcgacgg catttgtctt tggtctccaa
     9181 ggtaaaagac tgaaagtggt cgggtcgggt agtgcatata ggtcccgttc atctgcccga
     9241 gcatagcctt caattgcaga tccatcaaac cccatgcctc tcgttagggc attctctaac
     9301 tctaccacgg tgatagcaaa gcctttaata ttacccaata tatcggaaaa ccagaggcgt
     9361 atgaatttca catcgttttc tctggcttcg cggagaacgt attcaactgc ctcactgttt
     9421 ctttccaaca ttgggaaatc cttattagga agggcttgct atcttctatt tgttcttgta
     9481 attaaggaaa atataggcat taaagagtaa tcatttgtga aaaaagtgtc aaacaaaatt
     9541 cacacttgac cactgggaaa aataacgatg ctccactcct aaaaaaagag cggaacattc
     9601 tagaataact aggtcaatta tgcatcgaat gaaaggtgga attcatatgg atgcggtcgc
     9661 agcctcactg ggtcaacatc attttcagtt ttaaagctga tccaattttc aataacatct
     9721 tcggtgaata ccccaccctt tagtaaaaat tcatggtcat cctgtaaagc tttaagggcc
     9781 tcttcaagac ttcctggtac ttgcggaaaa gttgccgttt cctccgcaga cagttcaaaa
     9841 aggtcttgtt ggagcggttc ccctgggctg tagccgttct ctattccatc aatccctgcc
     9901 attaacatag cggcaaaagt aagataagga ttacaagttg ggtctgcaga cctgaattcc
     9961 actctttttg cggccggatt atcgaaatac attggaatcc tgcatgcagc cgatcggttt
    10021 ctagctgaca gcgccaaaat cgttggggct tcgaatcctg gtgttaatct tttataggaa
    10081 tttgtcgttg gggctgctaa cgccattaag gctttaccat gttttattag gccaccgata
    10141 taattgagag cgagttgtga taaaccggca tattcgtttc ctgcgaataa aggttcacca
    10201 tttttccata ttgattggtg ggtatgcata cctgtcccat tatcatcaaa tagcggcttg
    10261 ggcatgaagg tcactacttt tccatgttgt ttggcaacat tacgtaccgc atatttgtac
    10321 atcatcacat tgtcggcagt ttgaagcaga gggccaaacc gcatgtctat ctccgcttgg
    10381 cctccagtcg ctacttcatg atgttgagcc tctattctca ggccaaagtc attaaca
//
LOCUS       DASCNS010000294         4669 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002128105, whole genome shotgun sequence.
ACCESSION   DASCNS010000294 DASCNS010000000
VERSION     DASCNS010000294.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002128105"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(37..1068)
                     /locus_tag="QF838_10320"
     CDS             complement(37..1068)
                     /locus_tag="QF838_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJO61112.1"
                     /translation="MPFGGNDWLDLTIEETLEPDLPICDPHHHFWDHRLERIPYQEYL
                     LPQLVEDITSGHNVTSTVFIEARSMYRQNAPDHMKPIGEVEFVQGLAAASASGIYGDN
                     RAAAAIVGHANLNLGSEVREVLEALKEASPKRFRGIRHSVTWDPHPEIENTALHRSEG
                     QMLTKTFQDGAKVLADMGLTFEAWMYSHQLGELVNLAKTVPDLKIILNHVGGLVLDGP
                     YESIREEVLTNWRDRIKELSECPNVVVKLGGIGMPRTGFDWHLRQRPIGSEELAVQMG
                     PLLSYCIEQFGPERGMFESNFPVDKVSYSYNILYNAFKIFSKDYSPTERALMFRDNAI
                     DIYSVDESL"
     gene            1238..2149
                     /locus_tag="QF838_10325"
     CDS             1238..2149
                     /locus_tag="QF838_10325"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HJO61113.1"
                     /translation="MGTREWFKGNLHTHTTKSDGDETPEKVCEWFEKHDYDFLVLSDH
                     NHLTLLDYESTGKSNLKMIPGEEVTAFASSNMAPVHIGAIGISTLVEPVVCEDVVTTL
                     QMNIDHILEAGGIACINHPNFKWAFDHREMIEIEGAALFEVYNASRGCNNLGGEGKFS
                     TSEMWDHMLSNKKIIYGAATDDSHNYQDFAPDKHNPGRGWIMVRSESNDQDSLIKNME
                     SGNFYSSSGVSLDNLESSDHHIEFSISQIDDFLYSTKLIGENGRILKEVSGNKVRYEF
                     EGNEGYVRAEIMDSDGALAWTQPIFVL"
     gene            complement(2254..3030)
                     /gene="recO"
                     /locus_tag="QF838_10330"
     CDS             complement(2254..3030)
                     /gene="recO"
                     /locus_tag="QF838_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HJO61114.1"
                     /translation="MTSNRIYRTKAVVLKQMPIGETNRIITFYTRSAGKVAAVARGVR
                     RPGSKFGGSLEILNYVQTSIGRGRSLDNVNECLVIDSYKKLRENLTAIAEGIYVAELV
                     DTFGEHKSPNPRLFDILLETLSNLDKLEHREFWILWFEFRLLHVSGFLPEFMSCVECR
                     SSLDKSDHIFDPIAGGVLCPSCVDSHGSEHSWIVSVYALGILRFLQRSLNVTLGSAEA
                     SSYSDNLPELKSLLRIYMRYIAERDMKSTGFLDLVRFKES"
     gene            complement(3057..3668)
                     /gene="recR"
                     /locus_tag="QF838_10335"
     CDS             complement(3057..3668)
                     /gene="recR"
                     /locus_tag="QF838_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HJO61115.1"
                     /translation="MSTEYSSGTAPVQRLIEEFSRLPGIGPKSAQRLTYHLIRMPAEQ
                     AENLASAILSVKTRITLCSKCFHITESDPCSICLDTTRDQSKICVVEQPLDVMALEKT
                     STYDGLYHVLHGVISPINGIGPDDLKVKPLLQRLIDNDVTEIIMATNPNLEGEATSMF
                     IHKLIEPSGITITRPARGLPVGGDLEYADEATLGRAIEGRQNF"
     gene            complement(3670..3975)
                     /locus_tag="QF838_10340"
     CDS             complement(3670..3975)
                     /locus_tag="QF838_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HJO61116.1"
                     /translation="MNRNMMKQAQQLQKQMLKAQQEIEGTKVEFSSGGGVVKVVVSGK
                     MIVESIEIDPDAVDPEDVEMLQDLVLSAVNGAMAKAQELASSKMGAITGGLNIPGLT"
     gene            complement(3975..>4669)
                     /locus_tag="QF838_10345"
     CDS             complement(3975..>4669)
                     /locus_tag="QF838_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61117.1"
                     /translation="FRDVLTPALPLEIAIVESIMSHQNTESALNQFQETKTESTDDKK
                     SGEHILDKRAVSTEQKPIVARSINENETKDATTPPPVVPVTETYSESEPPELEKNKRT
                     PQIIASGDSIDEHWNELLRSLRQTGKRFNLGALLRGCKERKIKDDTLVLTFLHNSHLE
                     RVNSEIEDPLVNGQFESVVENVLGKKYKISVELASSEVKSVRGPIGQQSHLVRAAQSL
                     GARIVEERSKSQ"
BASE COUNT         1363 a         1024 c          905 g         1377 t
ORIGIN      
        1 acttggaggc ttcaaagaaa aaatccattc ccctctttat aaactctcgt caacgctata
       61 tatatctatt gcattatccc ggaacattaa agcgcgttcg gtaggagaat aatccttgga
      121 aaaaatttta aatgcgttat ataagatatt gtatgaataa gataccttgt ctacaggaaa
      181 attgctttca aacatacctc tttcaggccc aaattgttca atacaataac ttagaagagg
      241 ccccatttgg accgctagtt cctctgaacc gataggtctc tgtctcagat gccagtcgaa
      301 tccggtccgg ggcatcccaa taccgccaag tttaacaacc acgttagggc attctgacag
      361 ttcttttatt cggtccctcc aatttgttaa aacttcttcc ctgatagatt catatgggcc
      421 atctaggact aaaccgccga catggttaag tattattttg agatcaggaa ccgttttggc
      481 caaattaact aactccccaa gttggtgaga gtacatccaa gcctcaaaag taaggcccat
      541 atcagccaaa actttggcac catcctgaaa agttttggtg agcatctgcc cttcacttct
      601 atggagagct gtattttcta tttcaggatg agggtcccag gtaacggaat gtcttatacc
      661 tctgaacctt ttcggactgg cctcttttaa ggcctccaac acttcccgca cttcgctacc
      721 gaggttaaga tttgcatgcc ccacgatagc agcggctgct ctgttatcac cgtatatacc
      781 actcgcactt gccgcagcca aaccttgaac aaattccact tcacctatag gtttcatatg
      841 gtccggtgcg ttctggcgat acatagagcg agcttcaata aataccgtag aagttacgtt
      901 atgtccgctc gttatatcct caaccagttg aggtaacaaa tactcttgat aagggattct
      961 ttctaacctg tgatcccaaa aatggtgatg aggatcacat attggcaaat ccggctccag
     1021 ggtctcctcg atagtcaaat ccaaccaatc atttccacca aatggcatag ttagtactcc
     1081 atgctttaat catttttcac gatttcatgc aaatatacag gatttcaatg acaatgtggg
     1141 ttaggacatt ttcggtatcc taaaggttgt tatctgcaaa acgtataatt ggggtgttaa
     1201 gaaatttgaa acataaactt gcttaaaagg atttcacatg ggtactagag agtggtttaa
     1261 aggtaatctt cacacgcaca ctactaagtc agatggagat gaaactcctg aaaaagtttg
     1321 cgagtggttt gaaaaacatg actacgactt cttggtctta agtgatcaca atcatttaac
     1381 attactagat tacgagtcaa ctggaaaatc aaatctgaaa atgattccag gggaagaagt
     1441 tacggctttt gcttcttcta atatggcacc ggtacatatc ggtgctatag ggattagtac
     1501 actagtggaa cctgttgtct gtgaggatgt ggttaccaca ttacaaatga atatagatca
     1561 cattttagag gcaggcggga tagcatgcat caatcaccct aatttcaaat gggcgtttga
     1621 ccatagggag atgatagaaa ttgaaggggc cgcactgttt gaagtctaca acgcaagtag
     1681 agggtgtaac aatttaggtg gggagggaaa attctcgaca tcagaaatgt gggatcacat
     1741 gttaagtaat aaaaagatta tttatggtgc agcaacggac gattcacata actatcagga
     1801 tttcgctcca gataaacata accctggtag agggtggata atggtgcgtt cagaatcaaa
     1861 tgatcaggat tcgttgataa aaaatatgga gtcgggaaat ttctattctt caagcggtgt
     1921 ctcgctagac aatttggaaa gctcagatca ccacattgaa ttttccattt ctcaaataga
     1981 tgattttcta tattccacca aattaattgg tgaaaatggg cgaatattga aagaagtgtc
     2041 tggaaacaaa gtcagatacg aatttgaagg aaatgaagga tatgtacgtg cagagataat
     2101 ggattccgat ggagcattag catggactca gcccattttc gtcctatgaa atacctggct
     2161 tgtaacttag ctcacaagtc atttatatat ctgataatac caaaagccac caagtggact
     2221 agaaataaaa gaggacttaa agagattagc taattaactt tctttaaacc tgaccaaatc
     2281 caaaaaccca gtagatttca tatctctttc agctatatat ctcatataaa tgcgtaacaa
     2341 tgacttgagc tccggcagat tatcagaata agaagatgcc tccgctgacc ctaatgttac
     2401 gttcagtgac ctttggagaa aacggagtat gccgagggca taaactgaaa ctatccaaga
     2461 gtgttcgctc ccatgactgt caacacaaga agggcacaaa acgccacctg caattggatc
     2521 gaatatatga tcagatttat caagagagct acggcattcg acacaactca taaattccgg
     2581 tagaaatccc gaaacatgca acaatctaaa ctcaaaccat aatatccaaa attcacgatg
     2641 ctcaagttta tccagattcg atagggtttc caacaatatg tcaaacaatc gaggattggg
     2701 agatttgtgc tccccgaaag tatcgactag ctcagctaca taaataccct cagcaatggc
     2761 agtcaaattc tctctcaact ttttgtagga atcaataacc aagcattcat taacattatc
     2821 tagacttctc ccacggccaa tagaagtttg gacataatta agaatttcca agctgccacc
     2881 aaatttactt cccgggcgcc tcaccccacg cgctacggct gcaactttac cagcagaacg
     2941 ggtatagaaa gtaataatcc gattcgtctc accaatcggc atttgcttta gaacaactgc
     3001 ttttgtcctg taaattcgat tgcttgtcat cggtttgcct taccggatgg aatcatttaa
     3061 aagttttgac gaccttcaat agctctaccc aatgttgcct catctgcata ctctaggtca
     3121 ccgccaacag gaaggcctct ggcaggtctt gttattgtaa ttccagaagg ttcgatgagc
     3181 ttatgaatga acattgacgt ggcttcacct tccaaattag ggttagttgc cataattatt
     3241 tcagtcacat cattatctat cagtctctga agtaaaggtt taacctttag atcatcaggg
     3301 ccgatcccat tgattggtga gatgactcca tggaggacat gatataaccc gtcatatgta
     3361 gaggttttct ccaaggccat aacatccaaa ggttgctcaa ctacacatat tttgctttga
     3421 tccctcgttg tatctaaaca aatactgcaa ggatcgcttt ccgttatgtg aaaacatttt
     3481 gagcaaagtg ttatgcgagt cttgacggag aggattgcag aagccagatt ctcagcctgt
     3541 tcggctggca tcctaattaa gtgataagtc agtcgctgtg cggatttagg accgatccct
     3601 ggaagtctac taaattcttc tataagccgc tgaacaggag cagtcccgct agaatattcc
     3661 gtgctcaagc taagtaaggc ctggtatgtt caagcctccg gttatagcac ccattttaga
     3721 agatgccaat tcctgagcct tagccattgc accattaaca gccgataaaa caagatcctg
     3781 aagcatttca acatcttctg ggtccacggc atctggatct atctctattg attccactat
     3841 cattttcccc gagactacca cttttactac acctccaccg gaagaaaatt ctactttagt
     3901 tccttcaatt tcctgctgtg ccttcaacat ttgtttctgc aattgctggg cctgtttcat
     3961 catattgcgg ttcatcattg tgatttactc ctttcttcca ctatacgggc accaagtgat
     4021 tgtgcagctc gaactaaatg gctttgctgg ccaatgggac ctcgaaccga tttcacttca
     4081 ctactagcca gttcaacact aattttatac tttttaccta aaacgttttc gacgacactt
     4141 tcaaattgtc catttactag cgggtcttct atttcactat tcactctttc aagatgagaa
     4201 ttatgtagga aggttagcac caaagtgtca tccttaattt tccgttcttt acatcctcga
     4261 agcaaagcac ctaaattaaa tcttttaccg gtttgtctta aggagcgtag taactcattc
     4321 cagtgttcat caatgctatc cccagaagcg attatttgcg gagtcctttt atttttttct
     4381 aattcgggtg gctcactttc agaataggtt tccgtaactg gtacaaccgg cggtggagtg
     4441 gtggcatctt tcgtttcatt ctcgtttatg gatctagcta ctatcggttt ttgttcagta
     4501 ctaacagcac gcttatcaag gatatgttct ccacttttct tatcgtccgt actttccgtc
     4561 tttgtttctt gaaactgatt cagagccgac tcagtgtttt ggtggctcat aatagattcc
     4621 actattgcga tctccaatgg caacgccggg gtcaaaacat ctctgaagt
//
LOCUS       DASCNS010000295         5754 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002129684, whole genome shotgun sequence.
ACCESSION   DASCNS010000295 DASCNS010000000
VERSION     DASCNS010000295.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002129684"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="QF838_10350"
     CDS             <1..1002
                     /locus_tag="QF838_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61118.1"
                     /translation="VNENLASQEALLVDQEKALDKHQAELQNLKEIVTAFESNSANNS
                     ANTESSADREIKSVAAEVSTMSNQLENDMAAQGQHLEEEISMMHYRITSLEEQSAFIQ
                     TLLGDIQDEAEAIRYSISAISNEIEKSAEITGNLTHSFDAIVSTTEDFQGQLRDLEDE
                     TAFLNVAVEQSTSAIEQLDDALEEVEETQEELADSSNYPITVDQFTTYDNLIGNGLIG
                     DVLNSMPAPETMVVPDCTGPDGTRECIDKLVNSVNVDIDGTYESIQLSINDFCQAAEN
                     AYGLERVHGNRWDSTAPTDETDALLNAGFISESEFSIIQWSLKNRDILETEYPGTEC"
     gene            1337..2518
                     /locus_tag="QF838_10355"
     CDS             1337..2518
                     /locus_tag="QF838_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HJO61119.1"
                     /translation="MKVTGVKTYVIENEPGVRTGGGGQTEEWYIGGKYFLILELSTDE
                     GIVGIGEKLTGSSFTGKMTWKDFKSQIQLVHETVEAFVIGQDPFNIEKIYYEMYAGRH
                     DYRLPSLHFGMVISGIEMALWDIVGKANNQPIYNMLGGKCWDKLRAYAYMPPIPEGNP
                     ENAGTVAERLLAEGNTACKFDPFHPITGVPRDISLKEITYAENIFKNIRSAVGDELEV
                     GLGTHGQLTTYSAIRVAKAIEPYQPLWFEEPVSPENIDEMARVAAHTSIPIATGERLA
                     TVFQFSEVLRKQAAQIIQIDIGQAGGILVGKKVAAMAEASYAVIAPHMYCGPVAAAAA
                     IQVATCSPNFMIAEANQGSLHKKIFKDPIVFENGYIKIPTGAGLGVEFDPKVLEKHIV
                     S"
     gene            2682..3677
                     /gene="glnII"
                     /locus_tag="QF838_10360"
     CDS             2682..3677
                     /gene="glnII"
                     /locus_tag="QF838_10360"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HJO61120.1"
                     /translation="MAFKAEYIWIDGTEPSPSLRSKTRVLADESDFPIWGFDGSSTNQ
                     APGENSDCVLRPVFSCPDPISGGNNKLVMCEVLLPDMSPHPTNNRAACAEVASKFKHH
                     ESWFGIEQEFTLFEADGKQPIGFPDGGFPEAQGPYYCSVGANYNFGRGVSEAHATACI
                     DAGIGIAGTNAEVMPGQWEFQVGPLGPLEVSDQLWVARWLLHRVAEEFGIVVSIDPKP
                     VSGDWNGAGCHTNFSTKAMRESYQPIIDACEAIGKNINEHIANYGVGNEKRLTGLHET
                     QRIDQFSYGVSDRGASIRIPWQVEVDQKGYLEDRRPASNMDPYLVTKLLIQTTCG"
     gene            3902..4738
                     /locus_tag="QF838_10365"
     CDS             3902..4738
                     /locus_tag="QF838_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HJO61121.1"
                     /translation="MGKAPPHPTTLSSHEIDLEDYLLSLKNRGLSDNYIETSGNFLRH
                     YIQWLATNSATTNAESAEKYLSKSNHLKLNSRKRYADYLRKFLEFHGMTFDIKIKRPH
                     ILPEIVNEADIERLKDAITKASSWKISVTTHLALIETAVKTGMRRAELADLRVKDVDL
                     QRCRVKVVAGKGNKDRVIPIGAELCEMLARVCAELGEDERVFGMNKHSLGVWVRKWAT
                     KAGVNLHTHSFRHYFASTLATRGVNLRVIQELLGHSSLATTQVYLSVTADHLEDAIQL
                     LE"
     gene            complement(4746..5075)
                     /locus_tag="QF838_10370"
     CDS             complement(4746..5075)
                     /locus_tag="QF838_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61122.1"
                     /translation="MKDFNSTGFPLHKINKFLQGIFERTGRVALIAFRFYGFYSIRID
                     KPSVGPCTVGMVYEPEFLGNDERVKEHKDVIRGGVSWDAGFEAVGPEKVPNLYKKHCG
                     PTLPDYL"
     gene            complement(5325..>5754)
                     /locus_tag="QF838_10375"
     CDS             complement(5325..>5754)
                     /locus_tag="QF838_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61123.1"
                     /translation="LIWQMVLQRKFLETAHTDNEQSLTFESLGLTDKLLAVRMEEDFS
                     NVWSKRHSTEKNLNEVEFEKLNNYYLRWCTVMQTEWRMGRLTKEADMFYYKRKLWAML
                     NSICGREFYVREGRDFLRFEGLRELGDELYEELEGAPMPA"
BASE COUNT         1795 a         1171 c         1261 g         1527 t
ORIGIN      
        1 gttaacgaga atttggcaag ccaggaagca ttactagtag accaggaaaa ggcactggat
       61 aaacatcaag cagagctgca aaatcttaaa gaaatagtta ctgcctttga atcaaattca
      121 gccaataatt cagccaatac agaaagttcg gcagataggg aaattaaatc tgtcgcagcc
      181 gaagtttcaa ctatgtcaaa ccaattagaa aatgacatgg ctgcgcaggg acaacatctc
      241 gaagaagaaa tttctatgat gcattaccgc attacctctc tagaagaaca gtctgccttc
      301 atacaaaccc tgcttggaga tatacaagat gaagcggaag cgatccgata ctctatctca
      361 gccatatcaa atgagatcga aaagtcggct gaaatcacag gaaatcttac ccattcattt
      421 gatgcaattg tttctacaac ggaagatttc caaggacagt tgcgtgatct cgaagacgag
      481 acagcttttc taaatgtagc ggttgaacaa tcaacgtccg caatagaaca gttggatgat
      541 gctttagaag aggtggagga aactcaagaa gaattagcag actcctcaaa ctatccaatc
      601 acggtagacc aattcactac atacgataat ttaattggta acggactaat cggagacgta
      661 ttaaatagca tgccagcacc agaaactatg gttgtaccgg actgtaccgg cccagatgga
      721 actcgagaat gcatagataa acttgtgaat tctgtaaatg tggatataga tggaacttat
      781 gaaagcatac aactatcaat aaatgacttt tgtcaggcag cagaaaacgc ttatggactt
      841 gaacgggtac acgggaatcg gtgggactca acagctccaa ccgatgagac agatgcattg
      901 ctaaacgccg gattcatttc agaatctgag ttcagtatta ttcagtggtc attgaaaaac
      961 cgcgatattt tggaaactga atatcctggc acggaatgct gataggttaa gcctataggg
     1021 agggtgatta agtcctctct ctataattaa gagctctgtt aaagattact ccgccttgcc
     1081 aaatacagac gtaggaagtg tacttactac gaataggccc gcctttaagg cctcccaagt
     1141 aaaaagtgcc tgagttgcct ctatgggttc ccaagtgttt ataacaggca caacctcatc
     1201 cttctgagag tgccgtaggg ccctaacccc actcacggcc ttcttgtggt attggttcaa
     1261 attggtcgaa attgacacta ataccgaccc aatataaagt cacctgatat cgtgaatttt
     1321 gtaaaatgga gagcatatga aagtaacagg tgtaaagact tatgttattg aaaatgaacc
     1381 tggtgtaagg actggtggag gcggccagac ggaagaatgg tatataggtg gtaaatattt
     1441 ccttattttg gaactttcta ccgatgaagg aatagtggga atcggagaaa aacttacagg
     1501 tagctctttc actggaaaaa tgacatggaa ggatttcaaa tctcaaatac aattagttca
     1561 tgaaaccgtt gaggcttttg ttatagggca agatcctttc aatattgaaa agatctacta
     1621 cgaaatgtat gcgggaagac atgattacag attacccagc ctacattttg gaatggttat
     1681 atcagggata gagatggcct tatgggacat tgtaggtaaa gctaataacc aacctattta
     1741 caatatgctt ggtgggaagt gttgggataa actcagagcc tacgcttaca tgccacccat
     1801 tccagaagga aaccctgaaa atgcaggaac tgtggcagag cggttacttg cagaaggtaa
     1861 tactgcatgt aaatttgatc cttttcatcc gataactggc gttcctaggg atatcagttt
     1921 aaaggaaata acttacgccg aaaatatttt taaaaatatc agatctgctg tcggggatga
     1981 gcttgaagtt ggattaggta cacatggaca gttaacgact tacagcgcaa taagagttgc
     2041 aaaagcgata gaaccgtacc aaccactttg gtttgaagaa cctgtttcac ctgaaaatat
     2101 agatgagatg gctagggtgg ccgctcatac cagcatccca attgctactg gggagaggct
     2161 agccaccgtt tttcaatttt ctgaggtatt aaggaagcaa gctgcacaaa ttattcaaat
     2221 tgatattggt caagcaggag gaatattagt ggggaaaaaa gttgcagcta tggcggaagc
     2281 aagctatgcg gtaattgcac cgcatatgta ttgtggtcca gtggcagctg cagctgccat
     2341 acaggtggct acttgctccc caaactttat gattgctgag gcaaaccaag gatctttgca
     2401 caaaaaaata ttcaaggatc ctatcgtttt tgaaaacgga tatataaaaa tccctacagg
     2461 ggctgggcta ggagtagagt ttgaccctaa ggttttggag aaacatatag taagctgatc
     2521 aggctaaggt ttcttaccaa aagcagttac agacaaaaag atcctaaatt aacgaattgc
     2581 ttcgcataaa tttataaaat atcattcgac attgcaaaaa agttctaatt ttattacgtt
     2641 atatccgtag atttttaaat cacaaaattg aggtgcaaat aatggcattt aaagcagaat
     2701 atatatggat cgatggaaca gagccatccc cttcattaag atctaaaacc agggttttag
     2761 ctgacgagtc agattttcca atttggggat ttgatgggtc cagtaccaac caagcgccag
     2821 gagaaaattc agactgtgtc ttacgtcctg ttttttcatg ccctgatcca atttcaggcg
     2881 gaaacaataa attagtcatg tgtgaagttc tactacctga tatgtctcca catccaacaa
     2941 acaacagagc ggcctgcgcg gaagtcgctt caaaatttaa acatcatgag tcatggtttg
     3001 gcattgaaca ggaatttacg ttatttgaag cggatggaaa acaaccgatt ggattccccg
     3061 atggaggatt ccctgaagca cagggaccat actattgtag tgtgggagca aattataact
     3121 ttgggagagg agtatcagaa gcccatgcaa cagcttgtat cgatgcgggt ataggaattg
     3181 caggcactaa tgccgaagtg atgcctggtc aatgggaatt tcaagttgga ccccttggac
     3241 cgctagaggt ttctgatcag ttgtgggtag caagatggct actccatagg gtggctgagg
     3301 aattcggtat tgttgtatca attgatccaa aacctgtgtc aggtgactgg aatggtgcag
     3361 gctgtcatac aaatttttct acaaaagcaa tgcgagaatc ttaccaacca attatcgatg
     3421 cttgtgaagc catcgggaaa aacataaatg agcatattgc aaattatggc gtcggaaatg
     3481 aaaaaagact gactggactc catgaaactc aacgaattga tcaatttagt tatggtgtat
     3541 ctgatagagg tgcctcgata agaattcctt ggcaagttga agttgatcaa aaagggtacc
     3601 ttgaagatcg taggcctgct tcaaacatgg acccgtacct cgtaaccaaa ctactcatcc
     3661 aaacaacttg tgggtgaaat ttttctactg aggcaacaat tttaatatta acctcagtag
     3721 aaaaagaatg gttggtctag atggattatt gaagaggaac tggatcccaa ataaggaagc
     3781 ccatcgctaa gcggtcgttg gtgaaactta gaaagctgct gcaatctaat ggcataatgg
     3841 tggagctggg gaggatcgaa ctccctacct cttcaatgcc attaaagacc ccaccttcgg
     3901 tgtgggtaaa gcaccacccc acccaactac tctttccagt cacgaaatag acctagaaga
     3961 ctacttatta agtcttaaaa atcggggtct gagtgataat tacattgaga cttctgggaa
     4021 ttttctcagg cactatattc aatggcttgc caccaattct gccaccacta acgctgaaag
     4081 tgctgaaaag tacctctcaa aatccaatca tctcaagctc aacagcagaa agagatatgc
     4141 ggactatttg aggaagtttc tagaattcca cggcatgacc tttgatataa agattaagcg
     4201 tccccatata ctgccagaga tagttaacga ggcagacatt gagaggctga aagacgctat
     4261 caccaaagcc agctcttgga agatatcagt cactactcac ctcgccctaa tagagacggc
     4321 tgttaagact ggaatgcgcc gtgctgagct ggcagacctg cgagtcaaag acgttgattt
     4381 acaacggtgt cgcgtcaaag ttgtggcagg caaaggcaac aaagatagag taattccgat
     4441 tggggctgag ctatgcgaaa tgctggctag agtgtgcgct gagctgggcg aggatgagag
     4501 ggtgtttgga atgaataagc acagtctagg cgtatgggtt cgcaagtggg ctaccaaggc
     4561 tggcgtaaat ctccacactc atagctttcg gcactatttc gcctccacct tagcaactcg
     4621 tggcgtaaat ctgagagtaa tacaggaact tctggggcac tctagccttg ccaccacgca
     4681 ggtatatcta tcggtgacgg ctgaccattt ggaggatgct attcaattat tagaataaac
     4741 tattttcaaa gatagtccgg taaggtgggc ccacaatgtt ttttataaag attgggaacc
     4801 ttttctggcc caaccgcctc aaagccagcg tcccaagaaa cgccacccct tattacgtct
     4861 ttgtgttctt taacgcgctc atcgttgccc aaaaattcag gttcatatac cataccaacc
     4921 gtacaaggac ctacgctcgg tttatcaata cgaatggagt aaaacccata aaaccgaaaa
     4981 gcaatgagag caacacgccc ggtgcgctca aaaatacctt gtaaaaattt gtttattttg
     5041 tgaagaggaa acccagttga attgaagtct ttcatatgga accggatacc cgtcaacata
     5101 ccggatacta ggaaggaatg gatattactg gtttgtattc cacttggtgg tactgcgttg
     5161 tcaacaacgg tagtaattct attttatttt ctctaattta ctggctattt ctaaaaatag
     5221 tcacaataac tcgcacgtaa ttccactatt actgctaatc agtaagggac ttcgtctttt
     5281 acgcctatct acacatggag cctattgcca cacaaatttt tgaattatgc cggcatgggg
     5341 gcgccttcaa gctcctcgta caactcatcc ccaagttctc ttaaaccttc gaacctgagg
     5401 aaatccctac cttccctcac gtaaaattcc cggccacata tggaattaag catcgcccac
     5461 aatttacgtt tgtaatagaa catgtcggcc tctttggtta gacgacccat gcgccactct
     5521 gtttgcatga cagtacacca tcgaaggtag taattgttta gtttttcaaa ctctacttca
     5581 tttaaatttt tttccgtgct atgtctcttg ctccatacat tggaaaaatc ctcttccatc
     5641 cgaactgcta ataatttgtc cgtcaaacca agagattcaa atgtaaggct ctgttcatta
     5701 tctgtatgag cagtttccag gaattttctc tgtaatacca tctgccaaat taat
//
LOCUS       DASCNS010000296         6738 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002136066, whole genome shotgun sequence.
ACCESSION   DASCNS010000296 DASCNS010000000
VERSION     DASCNS010000296.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002136066"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="QF838_10380"
     CDS             complement(<1..347)
                     /locus_tag="QF838_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HJO61124.1"
                     /translation="MLTKRIIPCLDVDNGRVVKGVGFINLRDAGDPSELAKFYDREGA
                     DELIFLDITASSDNRDTMVEVVKEVSEEVFIPLTVGGGIRQVSDVRKMLEAGADKVSL
                     NTAALGDPNLVKES"
     gene            complement(364..918)
                     /locus_tag="QF838_10385"
     CDS             complement(364..918)
                     /locus_tag="QF838_10385"
                     /inference="COORDINATES: protein motif:HMM:NF017026.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJO61125.1"
                     /translation="MPTKTNQDSFIAAKKLDIYHITSLLPAMPYHEIADIGCAEGALS
                     VPLGKLVYRGNVVSLDPIKKNLTTTRRELKKARLTNVKATHITDEPTLPLKDESLDGA
                     MSAFPFQFFKKPEQTLKDANRCMKKGAWLSVIEWHPDFLEYGPASKNRVQPTDLRGKI
                     EKAGFKFYIRHNLSDMAYMMIFSK"
     gene            complement(941..1669)
                     /gene="hisA"
                     /locus_tag="QF838_10390"
     CDS             complement(941..1669)
                     /gene="hisA"
                     /locus_tag="QF838_10390"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HJO61126.1"
                     /translation="MKVIPAIDIRGGSCVRLLQGDYSQETIFAEDPLEMAIKWIQQGA
                     EYLHIVDLDGAREGSAVNMEIISKICSESAVPIQLGGGIRDYETAARYIETGVSRVIM
                     GTSAVDRTGELERILNTLGDESLIVSVDSREGLVALDGWTRDSEMTVEDVIENMEAIG
                     VKRCMYTDILKDGTLSEPNYEGVKNLLAFTKMKIIAAGGIASLDHLKQMALSGVEAAI
                     VGRALYTGDIKLREAIDELKYSNR"
     gene            complement(1666..2319)
                     /gene="hisH"
                     /locus_tag="QF838_10395"
     CDS             complement(1666..2319)
                     /gene="hisH"
                     /locus_tag="QF838_10395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HJO61127.1"
                     /translation="MNESSSDRPKIAVIDYQAGNLRSVEKALVRTGADALVTSNPSAI
                     ESCDGIVFPGQGACDSSMINLKNRSLDKLILQLIAAKKPFMGVCLGLQLLLETSEEGE
                     EKCLGLIAGTVKRFPEGKKIPHMGWNEVNIKSDHPVLATLPNNSHFYFVHSYYADPTD
                     KNIIAATTKYGVEFCSAVAFQNVVAVQFHPEKSGDIGLEIYKNFVEFTKASMGTSSQ"
     gene            complement(2312..2875)
                     /locus_tag="QF838_10400"
     CDS             complement(2312..2875)
                     /locus_tag="QF838_10400"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HJO61128.1"
                     /translation="MKIYTRAGDKGETGLFFGGRVSKNDPRCEAYGSVDHVVSAMGTA
                     RAFCKDEKVKRIIIDIQNQLFTVGAELATLPENYDTMKDSYKIIVPEMVIELEQLIDE
                     LDSEVKLPPSFILPGASPGSAVLDLARTALREAERRILDVEELGQLVNRQIIPYINRL
                     SDLLFMLARYEDRDLPDELITGQKINE"
     gene            complement(3107..3937)
                     /gene="moeB"
                     /locus_tag="QF838_10405"
     CDS             complement(3107..3937)
                     /gene="moeB"
                     /locus_tag="QF838_10405"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HJO61129.1"
                     /translation="MATKTPSLALTPYQVNRYSRHIIMPQVGSVGQRKLIDAKVLMVG
                     AGGLGSPISIYLTLAGVGTVGLVDFDDVDVTNLQRQILHFNDDIGRPKVDSAVETLKA
                     YNPDTNVNVHEEPISSINAMEIMKDYDIIVNGADNFPTRYLVNDAAYLLGKPLVDGSI
                     LLFDGQATTYLPGNGCYRCLFPTPPPPGEVPSCAEAGVLGMLPGMIGTIQATEVVKLI
                     LGVGDSLSGRLLLVDALSMEFRTVKLRRNPDCPLCGDNPTVTELIDYIAFCGGAPLTG
                     "
     gene            4276..5370
                     /locus_tag="QF838_10410"
     CDS             4276..5370
                     /locus_tag="QF838_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014045.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HJO61130.1"
                     /translation="MTDSNIALLVDYENVGVDSLQYLVEQVSGMGRIIIKRAYADWSS
                     QRKGQERLIELGIEAVHNYRSVKSGKNSCDIKLTIDAVEILHSGQVDSFVIVSSDSDF
                     VPLVTHLRGAGKGVIGAGRKAVTSTTMVKSCDKYIFLDLQSAPAVAQKSVPQTKNNYR
                     KPAPYRERKPVVSVPDGLDNEKSVSQLLMKAVEATAENDGNSLGTKLAQTMTRIDPGF
                     DYKELGFRSFRDFLASRDEIEVTFREGTDFTVSIRTNEAQTSNTHENSDPPRIPSVSP
                     RIISFQPEESNQDSSQLPEGWESEVDTAWKSKEKDQLSGQSAATQAAKVLGFQRLRDS
                     LYPTIDKLLESSNLLKSNWARNKNYVTRKK"
     gene            5390..6157
                     /locus_tag="QF838_10415"
     CDS             5390..6157
                     /locus_tag="QF838_10415"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HJO61131.1"
                     /translation="MLIAIDIGNTNITIGLFRESELVSTWRFSTDSQKTSDEYGLLLK
                     QVMSYNNVEIAQIDAATICSVVPPLTTTFESLCRIYIDVNPLTVVAGTKTGIKVLYDS
                     PRDVGTDRIVDAAAAHHIYGGPCIIVDLGTATVFDAVNERAEYLGGAIAPGMIVSADS
                     LYRATSQLRKVELNAPGQAIGKNTTHSIQSGLVFGYSELIKGMIARFKKELGTEATVI
                     ATGGQATIVKDQVGEFDYIDPDLTLKGLQIVHSLNIN"
     gene            6168..>6738
                     /locus_tag="QF838_10420"
     CDS             6168..>6738
                     /locus_tag="QF838_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HJO61132.1"
                     /translation="MPSPLNNKRVVLGVTGSIACYKAADLASKLVQSGALVDVVLTSA
                     ATQFVSELTFRSITHRSVFTDLYSPESELSINHVALAETADIIIVAPTTANTTAKIAN
                     GISDDVLTTTILATRAPIIIAPAMDANMFDNAATQANVTKLVTRGVFIAGPANGRLAS
                     GLIGQGRMVEPSDIISSANMILGKDGDLSG"
BASE COUNT         1996 a         1546 c         1266 g         1930 t
ORIGIN      
        1 gattccttaa ctaaattagg gtctcctaga gccgcagtat ttaatgagac tttgtctgct
       61 cctgcttcta acatcttcct tacatctgat acttgccgaa taccaccacc aacggtcaat
      121 ggaataaaca cttcttctga cacttccttg acaacttcaa ccattgtgtc tctattgtct
      181 gaggatgcag ttatgtctag aaatatcagt tcatccgcac cttctctatc gtaaaattta
      241 gccaattcag atggatcacc agcatctctc aagtttataa aaccgacacc ttttaccacc
      301 ctaccgttat caacatccag gcacggtatt atcctctttg taagcatctc attgacctat
      361 cgatcatttg gaaaaaatca tcatataagc catatctgac aaattgtgcc taatgtagaa
      421 tttgaatcct gctttttcta ttttccctct caggtcagtt ggttgaaccc ggtttttact
      481 ggcaggtccg tactcaagaa aatctggatg ccattctata actgacaacc aagcaccttt
      541 cttcatacat ctattagcgt ctttcaaggt ttgttcgggc ttcttaaaaa actggaaggg
      601 aaatgctgac attgcaccat ccaagctttc atccttaagg ggaagtgtag gctcatccgt
      661 tatatgagtc gccttcacat ttgtcaatct ggcttttttt aactcacgcc gtgtagttgt
      721 aaggtttttc ttgataggat ccagtgatac tacatttcct ctataaacca attttcccag
      781 aggaaccgaa agggcccctt cagcacatcc tatatctgca atttcatggt aaggcatagc
      841 aggaagaagc gaagtaatat gatatatatc cagttttttc gctgctataa aagagtcttg
      901 gttcgttttt gtcggcatgt attgcaccaa ttattttttt ctatctatta gaatatttta
      961 actcatcaat tgcttctcgc aatttgatat ctccagtgta caaggctcga ccaactatag
     1021 ctgcttctac acctgataac gccatctgtt taagatggtc aaggctagct atgccccctg
     1081 ccgctattat tttcattttc gtaaatgcca aaagattttt gactccctca taattgggct
     1141 cactaagagt cccatccttt aatatgtcgg tatacataca tctcttaaca ccaatcgctt
     1201 ccatattttc aattacatct tctactgtca tttcactatc tctagtccaa ccatctaatg
     1261 caaccagccc ctctctagaa tctacgctga ctatcaaaga ttcgtcaccg agcgtattta
     1321 atatccgctc taattcgcct gttctatcta cagctgaagt tcccatgatt actcgtgaaa
     1381 ctccagtttc aatatatctt gcagccgtct cgtaatcacg aataccccct ccaagttgaa
     1441 tcggcacagc agattccgag catatttttg aaattatttc catgttcaca gcagagcctt
     1501 cgcgtgctcc atctaagtcg acaatatgta gatattcagc tccctgctgt atccatttga
     1561 tagccatttc caatgggtct tcagcaaaaa ttgtctcttg agaataatcc ccctgaagca
     1621 atctgacaca agaacctcct cgaatatcaa ttgcaggtat cactttcatt gagatgatgt
     1681 acccatggag gctttagtaa attcaacaaa atttttatat atttcaagtc ctatatcacc
     1741 gcttttttca gggtgaaatt gaacagcgac cacattttga aatgcaacgg cactacagaa
     1801 ctccacacca tattttgtag tggcagcaat tatgttttta tcagtggggt ctgcataata
     1861 gctgtggaca aaataaaaat gagaattatt tggtagagtt gctaatacag ggtgatccga
     1921 ttttatattt acttcgttcc atcccatatg tgggatcttc tttccctcag gaaaccgttt
     1981 tacagtacct gcaatcagac caagacattt ttcttcacct tcctccgaag tttccaaaag
     2041 aagttgcaat cccaaacaaa cccccataaa aggttttttt gctgcaatca attgcaatat
     2101 caacttgtct agagaccggt ttttcaaatt aatcatagaa ctatcacatg cgccctggcc
     2161 gggaaaaacg attccatcac aagattcgat agcacttgga tttgacgtta ctaaggcatc
     2221 agcgccagtt cgaaccaatg ccttttcaac acttcttaaa ttccctgcct gataatcaat
     2281 aactgctatt ttaggcctgt ccgaactgga ttcattcatt tattttttgg cccgttatta
     2341 attcatctgg taaatcccta tcctcatacc ttgccagcat aaatagaagg tctgaaagcc
     2401 tgttgatgta gggtattatt tgcctgttca ccagttggcc caattcctcc acatcaagga
     2461 ttctcctttc agcctcacgc aacgcagttc ttgccaaatc taaaaccgcg gaaccgggtg
     2521 aagccccagg taaaatgaaa cttggtggta attttacttc tgagtccaac tcatcaatta
     2581 attgttcaag ttcaattacc atttcaggaa cgataatttt gtatgagtct ttcatagtgt
     2641 catagttctc aggcagggta gctaattctg ccccaactgt gaaaagctga ttctgtatat
     2701 caatgattat tcttttgact ttctcatcct tgcagaaagc acgagccgtt cccatagcag
     2761 atacaacatg gtcgactgag ccgtaagctt cacaccgtgg atcatttttg gaaaccctgc
     2821 caccaaaaaa tagccccgtc tcccctttat cgcctgctct ggtatatatt ttcacgatat
     2881 cgaattccta aaggtgtctt aaggtaacac tgtatatggc tgaatataat atctctcact
     2941 caacgaacag taaacggaat atatggtcca aagatacgca tcggtagtaa tctacctgtc
     3001 catatagtct caacagactg tttcattcaa ttccgtagaa gaattggaag acccaattgt
     3061 gaactacatg atgtagcctg tcctaaactt accaccaaat cggaaatcat cctgtcagtg
     3121 gggcaccgcc acaaaaggct atgtagtcaa tcaactctgt tacggttgga ttgtctccac
     3181 ataacgggca atctggattt ctcctaagtt ttacggttct aaattccata cttaaagcgt
     3241 ctacaagcaa aagccggccg cttaatgaat ctccaactcc gagaatcaat ttcactacct
     3301 cggtagcttg tatagttcca atcatacctg gaagcatccc caaaaccccg gcttcagcac
     3361 aactaggaac ttcccctgga ggaggaggtg ttggaaatag gcatcgatag caaccattac
     3421 ctggcaaata ggtcgtcgcc tgtccatcga ataacaaaat actaccgtca accagaggtt
     3481 tccccagaag ataagccgca tcattaacca aatatctagt agggaaatta tctgcaccat
     3541 tcacaataat gtcataatct ttcattattt ccatggcatt tatcgatgaa ataggttctt
     3601 catgcacatt cacattggtg tcagggttat aagctttaag ggtttcaaca gccgaatcaa
     3661 ctttgggcct accaatatca tcattaaaat gaagtatctg ccgctgcaag ttagtcacat
     3721 caacatcatc gaaatcaacc aacccgacag taccaacgcc agctagcgtt aggtatattg
     3781 aaatagggga tcctaatcca cccgcaccaa ccatgagtac tttggcgtca atcaatttcc
     3841 tctgaccgac actccctacc tgtggcatta tgatgtgtct gctgtatcta ttgacctggt
     3901 atggggtcag tgctagtgat ggtgtttttg tagccaaaac tatcctccat aaattttctc
     3961 gttaatatat agagccccgc taccaacgct tcaaagcttt gggcggggct ctatatacac
     4021 ctgcggccta gttttacagc gttgacagcc ttaaatctag ttcctacagg aacaggttat
     4081 tttcaacacc tttttataca tgaaattcat cattttcaga attatagcat cacgtaattt
     4141 aggcatcaac taacggcaat ataactaaac atagtgttta aattatgatt taacaacaat
     4201 taagagtatc tctggatggt ataattggag acgcttttta acttttctta cacttagccg
     4261 agcggcggga ttccattgac tgattcaaat attgcattat tagtagatta cgaaaacgta
     4321 ggagttgatt ccctacaata cctggtggaa caggtatctg gtatgggacg catcatcata
     4381 aaacgtgcct acgccgattg gtcatcacag agaaaaggtc aagaaagatt gattgaacta
     4441 gggatagagg ctgtacataa ctacagatca gtgaaatcgg gcaagaactc ctgcgacata
     4501 aaacttacca tcgatgccgt agagattctt catagtgggc aggtcgattc ttttgtcatt
     4561 gtgtcctcag atagtgactt cgtgcctttg gttactcatc tgcggggtgc tggtaaaggt
     4621 gtaataggtg caggtagaaa agctgtaaca tcaaccacca tggtgaaatc atgcgacaag
     4681 tacatatttt tggatcttca atctgcccct gccgtggctc aaaaatcagt accccaaaca
     4741 aaaaacaatt acagaaaacc cgcgccatat cgagaacgaa agccagtcgt ttctgtaccg
     4801 gatgggttag ataatgaaaa atctgtcagt caattgctaa tgaaggctgt agaagccacc
     4861 gccgaaaacg atggcaactc attaggaacg aaattggccc aaactatgac tcgtatagac
     4921 ccagggtttg actataaaga actcggattt agatcattta gagatttttt agcctcaagg
     4981 gatgaaatag aagtcacctt tcgcgaaggc accgatttca cggtgtcaat cagaactaat
     5041 gaggcacaaa cttccaacac ccacgaaaat tctgaccccc ctcgtatacc ttcagtaagt
     5101 cccagaatta ttagttttca accagaggag tcaaatcaag attctagtca attgccggaa
     5161 ggctgggaat cagaagttga tacagcttgg aaaagtaaag aaaaagacca attgtcgggc
     5221 caatccgcag ccactcaagc agcaaaagtt ttgggcttcc aaagactgag ggattctctg
     5281 tacccgacta ttgataaact acttgagtca agtaacctgt taaaatcaaa ttgggctaga
     5341 aacaaaaatt acgtgactcg caaaaaataa ctgagatcat gctgacacca tgctgattgc
     5401 tattgatatt ggtaatacca acataacgat aggcctattt cgagaatctg aacttgtctc
     5461 aacatggaga ttctcaactg actcgcaaaa gacctcagat gaatatggtt tgttgctaaa
     5521 gcaagttatg tcatacaaca acgtagaaat tgctcaaatt gacgcagcaa ctatctgtag
     5581 cgtggttcct cctctgacaa ctactttcga atccctgtgt aggatatata ttgatgtcaa
     5641 cccactgaca gttgtggcag gaaccaaaac tggaataaag gtcctctatg atagccccag
     5701 agatgtggga accgatcgaa tagtagatgc agcggcggcg caccatatat acggtgggcc
     5761 atgcatcata gttgatttgg gaaccgccac ggttttcgat gcagtcaacg aacgagcgga
     5821 atatctcgga ggagcaatag cccccggaat gattgtttct gccgattctc tttatagggc
     5881 cacctctcaa cttagaaaag tggaactaaa cgccccaggt caagcgatcg gaaagaatac
     5941 gacccattcg atacaatctg gtcttgtatt cggttattca gaacttataa aaggtatgat
     6001 agccagattc aaaaaagagc taggaactga ggcaactgtt attgcaacag ggggtcaagc
     6061 aaccatagta aaagaccaag ttggggaatt tgattacata gatccggacc tcactcttaa
     6121 gggtttgcaa atagttcatt cccttaacat taactaaagg tagcgaaatg ccatctcctc
     6181 ttaacaacaa aagagtcgta ctgggcgtaa cgggtagcat agcgtgctat aaagctgctg
     6241 acctcgcgag caaacttgtt cagagcggcg cattagtaga cgtagtactg acaagtgctg
     6301 caacccaatt tgtatcagaa ttgactttca gaagtataac tcacagatcg gtatttaccg
     6361 acctatattc accggaatct gagctttcaa taaaccatgt agcactcgcg gaaaccgctg
     6421 atataataat agttgcccct acaacagcga acacaacagc caaaattgcc aatggtattt
     6481 ctgatgatgt tctgaccacc actattttag caactagggc tccaattatt atcgcccctg
     6541 caatggatgc caacatgttt gataacgcag ctacccaagc aaatgtgacc aaacttgtaa
     6601 ccaggggagt ttttattgca ggcccagcca atgggaggct tgcctcaggc cttatcggac
     6661 aaggaaggat ggttgaaccc tctgacataa ttagttcggc caatatgata ttaggtaagg
     6721 atggggactt gtcaggta
//
LOCUS       DASCNS010000297         4401 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002136955, whole genome shotgun sequence.
ACCESSION   DASCNS010000297 DASCNS010000000
VERSION     DASCNS010000297.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002136955"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="QF838_10425"
     CDS             complement(<1..231)
                     /locus_tag="QF838_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HJO61133.1"
                     /translation="MALKPLILEMGTGIDLHGQNATEAARRAVWNAVHQSSIMGLGLF
                     GPDTSKNMVVEITIAITRPDEVDEDVVLAVLPH"
     gene            456..530
                     /locus_tag="QF838_10430"
     tRNA            456..530
                     /locus_tag="QF838_10430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:488..490,aa:Gly,seq:ccc)
     gene            561..1844
                     /locus_tag="QF838_10435"
     CDS             561..1844
                     /locus_tag="QF838_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJO61134.1"
                     /translation="MSNLTIRDVRTIFTEPDNIRLVIVKIETNEPEIYGVGCATFTQR
                     PTPVMAAVEDYLKPFLIGKDPADIEDIWQSAYVSSYWRNGPVLNNALSGIDQALWDIK
                     GKLAGMPVYDLLGGKARSSAAVYVHANGTEYQEVEDDVHMYIEEGFHHIRVQVVTPGY
                     VTYGNKGSSDEATSPKSGPRLGTDRIFEPIPGKLHAHPGGIFEPGPYIESALGLFEHI
                     RSKIGWDVEILHDVHERVPPIQGVGFAKEVEPYKLFFLEDLFSPEDNEYFRLVRQQTS
                     TPIAMGELYNNPHEVIPMIKDRLLDFLRIHISQIGGITPARKLAALCEAFNVRTAWHG
                     PGDTSPIGHAANLMLDLNTINFGIQEYAIFGENTKEVFPGCPEVKDGYMWPNGLPGLG
                     IDIDESLAAKFPFKDRAYGGAWDTVRRADGSVVKP"
     gene            1898..2662
                     /locus_tag="QF838_10440"
     CDS             1898..2662
                     /locus_tag="QF838_10440"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HJO61135.1"
                     /translation="MASEIDFNCDMGESFGMYKMGFDEEVIKYISSANIACGFHAGDP
                     MWMRKTVELAEQHGVAVGAHPSYPDLNGFGRRNMNATPQEVRNDVVYQAGALKAFTSD
                     RNLQHVKPHGAMYNQAVDDADLGKSICEAVLEVDSDIILLALAGSTWIKVADDLGMRV
                     AREVFADRAVHSDGTLVSRSKEGSVIHDTNEVVERSLRMVTEGKILSVEGDIVEVEAD
                     SICLHGDTPGAVDMAKTLKQEFHAAGVQVKQLRDIV"
     gene            2686..3423
                     /gene="pxpB"
                     /locus_tag="QF838_10445"
     CDS             2686..3423
                     /gene="pxpB"
                     /locus_tag="QF838_10445"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HJO61136.1"
                     /translation="MNGTLLNEQPQFAAMGDSALMVTVGDSISYDINKKVHDIISLIK
                     LEDWPGIKEIVPTYSSFVVHFNPIIFSMEGLVECVQGIWNDDLNSENTDSRIVLIPTL
                     YGSEYGPDLEFVSEMTGLPIQEVIEIHSKVDYLVYALGFSPGFPYLGGLDSKIHCPRL
                     ETPRVSVPQGAVAIADSQTGVYPVTSPGGWRLIGRTPVKMFDPSRANPSLVTPGDYIR
                     FVPIGSDEEFAEILEKSAQGTFEVEVQ"
     gene            3420..>4401
                     /locus_tag="QF838_10450"
     CDS             3420..>4401
                     /locus_tag="QF838_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HJO61137.1"
                     /translation="MIEIAKAGTLTTIQDAGRTGYQELGVPESGALDNFAFRCANLLV
                     GNAENTPALESVLFGPEIKFLKDTFIAITGADLGPVLNGDKVNLWETLEVSSDSILTF
                     SGPTGNGLRSYLALAGGVAGAGTDLVMGSYSTYIAGEFGGHEGRALRDGDVINTGAGL
                     EGFEGGLSLENPPNYDEDVVIRIIEGPQHDRFSETAMETLTSSSYQVTPNSDRMGCRL
                     DGPILEHLTGPDVISDGNAFGVIQVPGDGKPIILLADRGTTGGYTKIATVITADRSKL
                     AQLLPGSSIKFEIVGQTKAEELLREQETLLASFLNKEGSPLKFKVDGMLVD"
BASE COUNT         1262 a          811 c         1082 g         1246 t
ORIGIN      
        1 gtgaggtaaa accgccaaaa ctacatcttc atccacctca tctggccgag ttattgcaat
       61 cgtaatttca acaaccatgt tttttgaagt gtcaggacca aataaaccca accccatgat
      121 gctactctga tgcaccgcat tccagacagc tcttcttgca gcctcagtcg cgttttgacc
      181 atgtaaatcg attcctgtac ccatctcaag tattaaaggc ttcagtgcca tatttattct
      241 ccgatctaag ttgttagatg gcttttgatt aaccgtaatt ctaactgatc gagctactgc
      301 tgtggatttt tgtcatattt aaacagcctc tgttaaatgg gataagaact tttcgacatt
      361 tttgtgacat atgttttcat ttttgatcac ctacccggct ttagaaataa tcactggatt
      421 tcgtagctaa atttgtccta taatgggttt tccaggcggc agtagctcag gggtagagca
      481 tttgcttccc aagcaaaggg ccgtgggttc gaatcccatc tgccgctcca atatgacact
      541 attttttcgt cgagatattt atgagtaatc tgacaattag ggatgttcgg acaattttta
      601 ctgaacccga taatatccga ttggtgatag taaaaattga aactaatgaa ccggagattt
      661 atggggtagg gtgcgctact tttacccaga gaccgactcc tgttatggct gccgttgaag
      721 attatctaaa gccatttttg attggtaaag accctgctga tatagaggac atatggcaat
      781 ctgcttatgt ttcatcgtat tggcgaaatg ggccggtttt aaataacgca ttgagtggga
      841 tcgatcaggc gttgtgggat attaagggta aattagccgg aatgcctgtt tatgatttac
      901 ttggaggaaa ggccagaagt tcggccgcgg tttatgtaca cgccaatggt actgaatatc
      961 aggaagttga ggatgatgta catatgtata tcgaagaagg gttccaccat attcgtgtgc
     1021 aggttgttac ccctggatat gttacatacg gcaataaagg atcttctgac gaagctactt
     1081 ccccaaaatc tggtccaaga ttgggaacag ataggatatt tgaaccgata ccaggtaaat
     1141 tacatgctca tcctgggggt atatttgaac ctggacctta tatagaatct gctttaggac
     1201 ttttcgagca cataagatca aaaattggct gggacgtgga aatactccat gatgttcatg
     1261 aacgtgtacc ccccattcaa ggagttgggt ttgcaaaaga agttgaaccg tacaaacttt
     1321 ttttcctgga agacttgttt tcgccagaag ataatgaata tttcaggctg gtaaggcaac
     1381 agactagtac tccgattgct atgggagagc tttataacaa cccacatgaa gttattccaa
     1441 tgattaaaga taggctgtta gattttttaa gaattcatat ttcacaaatt ggtggaatta
     1501 ctccagcccg gaaattagca gctttgtgtg aggccttcaa tgttaggacc gcctggcatg
     1561 ggccaggaga cacttctccg attggtcatg cagctaatct gatgttagac ctcaacacta
     1621 taaacttcgg aatccaagaa tatgcaatat ttggagagaa taccaaagaa gtgtttccag
     1681 gatgtccaga agtgaaagat gggtacatgt ggccaaacgg attacctggc ctgggtattg
     1741 atatcgacga gtctcttgcg gcaaaattcc catttaagga tagggcttat ggaggtgctt
     1801 gggacacagt acgcagagct gatggtagtg ttgtaaaacc gtaggcgtag aaaagtaaaa
     1861 tgtgaccacc attttggttg ggtaaaggaa agtaataatg gcttcagaaa tcgactttaa
     1921 ttgtgacatg ggtgaaagtt ttggcatgta caaaatgggt tttgatgaag aggttataaa
     1981 atatatttct tctgctaata tcgcctgcgg gttccatgct ggtgacccaa tgtggatgcg
     2041 taagacggtc gaactcgccg aacaacatgg tgtagcggtt ggtgctcatc cgagctaccc
     2101 ggacttgaat ggatttggac gaagaaatat gaatgccacc cctcaggagg ttcgtaatga
     2161 tgttgtctac caagcaggcg cattaaaggc gtttacatcg gaccggaacc tgcagcatgt
     2221 gaaaccccat ggggcaatgt acaaccaagc tgtagatgat gcagatttgg gtaaatcaat
     2281 ttgtgaagct gttcttgaag tcgattcaga tatcattttg ctagctcttg cagggtctac
     2341 gtggataaag gtcgctgatg atttagggat gagggttgcg cgcgaggtat tcgctgatag
     2401 agccgtccat agcgacggaa ctttagtttc tcgatcaaaa gaaggctctg taattcatga
     2461 taccaatgag gtggttgaaa gaagtttaag gatggtaact gaaggcaaaa tcctttcggt
     2521 tgaaggggat attgtagagg tagaagcgga ctcgatttgc cttcatggag atactcccgg
     2581 tgcagtggat atggcaaaaa ctctaaaaca agagtttcac gccgccggag tccaagttaa
     2641 acaactgaga gatattgttt agtagaacct aggtgaggat atttaatgaa cggaactttg
     2701 ctaaatgaac agccacaatt tgctgccatg ggagattctg ccctgatggt aacggtaggg
     2761 gattccattt cctatgacat taataaaaag gtacatgaca ttatttcact gataaagtta
     2821 gaggactggc caggtatcaa ggaaattgtg cccacataca gttccttcgt tgttcatttc
     2881 aatccgataa tattttccat ggaaggtttg gttgaatgtg ttcaagggat ttggaatgat
     2941 gatttaaact cagaaaacac ggattcaaga atagttttga taccgacttt gtatggttca
     3001 gaatatggcc ctgacctcga attcgtatct gaaatgacag gattgccaat tcaggaagtg
     3061 atcgagattc actcgaaagt ggattacctg gtgtacgccc taggatttag tccaggattt
     3121 ccatatttgg gagggttgga ttcaaagata cattgtccca gattggagac tcccagagtg
     3181 agtgttcctc aaggagccgt tgcaatcgca gattcacaaa cgggcgtata tcctgtgact
     3241 agcccaggag gttggagatt gattgggaga acacctgtca aaatgtttga tcccagtagg
     3301 gccaatccat cgttagtaac cccaggtgat tacataaggt ttgtccctat aggatctgat
     3361 gaggaatttg cagaaatatt agaaaaatcc gcacaaggca cctttgaggt tgaagtccag
     3421 tgattgaaat tgctaaggca gggacattaa ctactattca agatgctggt agaacggggt
     3481 atcaggaatt gggcgttcct gagtcggggg ctttagacaa ttttgccttc agatgtgcaa
     3541 atctgcttgt tggtaatgcc gagaatacac ctgcactaga atcagtttta tttgggccag
     3601 agatcaagtt cttgaaagat acattcattg ctataactgg agcggatcta ggtccggtat
     3661 taaatggtga caaagttaat ttgtgggaga ccttggaagt ctcgtcagat tcaatcttga
     3721 ctttttctgg tccgaccggc aacggccttc gttcgtattt agcgctagca ggaggagttg
     3781 caggagcggg aacagattta gtgatgggca gctactcaac atatatagct ggggaattcg
     3841 gcggtcatga aggcagggct ttaagagatg gagatgttat aaatacgggt gcgggccttg
     3901 aaggttttga aggtggttta tcactagaga accctcccaa ttatgatgaa gatgtagtca
     3961 ttaggataat tgaaggaccc caacacgata ggttttctga gacagcaatg gaaactctca
     4021 cctctagttc ctatcaagtg acccctaatt cagatcgcat gggctgccgt ttagatggac
     4081 ctatattgga acatttgacc ggacctgatg tgatttcaga tggaaatgcg tttggcgtaa
     4141 ttcaagtacc tggcgacggt aagccaataa tattacttgc tgatagaggt acgactgggg
     4201 gatacaccaa gatagcgacc gtgattactg cagataggag taaattggca caattactac
     4261 caggatccag catcaagttt gaaatagttg gacaaacaaa agctgaggaa ttattgagag
     4321 agcaagaaac attgctagcc tcattcctta acaaggaagg ctcgccttta aaatttaaag
     4381 tagatggtat gctagttgat g
//
LOCUS       DASCNS010000298        14594 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002141677, whole genome shotgun sequence.
ACCESSION   DASCNS010000298 DASCNS010000000
VERSION     DASCNS010000298.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 14594)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14594)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14594
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002141677"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="QF838_10455"
     CDS             complement(<1..478)
                     /locus_tag="QF838_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024900.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HJO61138.1"
                     /translation="MEVFINGKEIQVPEHSSVLDSINISGTAISQLCKDPDMKPIGAC
                     RTCLVEIEGVRGYPASCSTPILQGMKISTTGNKLDLIRKSVLELTAGMVDSDKDDYRE
                     LSRVMESNSINGQVWKSRSREETDASNPIFNIAMDSCILCGRCAQACQTGHQFIGAI"
     gene            complement(478..2004)
                     /locus_tag="QF838_10460"
     CDS             complement(478..2004)
                     /locus_tag="QF838_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013662.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HJO61139.1"
                     /translation="MNSQDFITSVANRAVKNPAEILVQVAQCSIAVGAQEIYRTLEER
                     LPASIAVDYAGCDGACFAGPKIHSEKDVSEYLEGVRVTSTGDSNVSNYINSQTRIVLE
                     HAGNISACDIYGYITNKGFSALAKVLSQPPEQTVDMVEESGLRGRGGAYFPVSIKWRS
                     AISTVSKERHLVINAEEGEPGVFKDRHVMESVPFRLIEGALIAAYSTEATSIRLYVNA
                     EAHLSYQRMLNALEICRSKGFLGPNIMGSRFTIDIEILKGAGGYVCGEESTLLNTIEG
                     KRREPRLRPPFPTESGLNQQPTIINNVETLCNLPWIVLNGPDEFKKIGDLDTPGTKIM
                     SISGSVSTPGVAELPMGSNLAELMVKFGGIASINALSAIAVGGPSSGVIPSSESLNLS
                     IGPGFIDGKSVMLGAGGVIGLSQDTNVLDVVRKLSEYNAKESCGKCTPCREGTSRLLE
                     LMANDSGDDPINWDGIGQLAEVINAASLCGLGQAAGNPVLSYGSYFQEVSPGSQRGGI
                     "
     gene            complement(2006..2473)
                     /locus_tag="QF838_10465"
     CDS             complement(2006..2473)
                     /locus_tag="QF838_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HJO61140.1"
                     /translation="MLNRSNMSSNNEERKRRNDLRRELIDKFPRDRSYLLPALHFLQE
                     KFAYLPDWGLEVLGWHLGVPASEVYGTATTYTELLLSPPAQKTVSVCDGVACRENGAT
                     QILEFLSNKTNSDLKVQKTDCAFLCSVAPAVSVNHRWFGRQVPGDVLLRLTEN"
     gene            complement(2467..4269)
                     /locus_tag="QF838_10470"
     CDS             complement(2467..4269)
                     /locus_tag="QF838_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013481.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HJO61141.1"
                     /translation="MFGYHNKILIIDVSSKSWRWEDLQEEVLKDFIGGSGLGAYLLFN
                     YCPPRTDPFSPENPLIFCASPLVGSRLTTTSKFAVVSKSPQTGFIGDSLSSSSLALSI
                     KGIGADAVIILGSSNQWLSLKVDESGIQFNDASHLIGNTTSTTEGHLIETLGPGYGTA
                     CIGPAGENMVRFASISNDGGRQAGRTGNGAVMGSKKIKAISFKGYKKLNYFNELSLSE
                     TRRQLAIKSLGEATEKYRTLGTMANLSVFERLKVLPIGNFRNQSNKDFQSISGEAIHN
                     EEKVSTSHCANCTIGCEKLVTIPNGGSAKVRLEYQSLFALGPLVGISDRETILKSSKF
                     CDEMGMDTISAGSTCAWFMEAMQKGLIPKRWLELLPIYENDEDLLTKLLNAISERSGI
                     GDILAEGSARASKEIGLGTEDFAMHVKGLEMPGYEPRSLHTMALALAVSTRGACHNRS
                     SAYEYDFASSSNGGKSSKGRMVVEGEDYSAVMDSLIWCKFVRKVFDEFYVESSEILNN
                     VTGWDMDPNQLARCGERINNLKKQFNIREGWVKDQDNLPKRIFTEPLSDDENRQSLSE
                     SDLGIMIKSYYDAREWTSDGLIPESKLKSLGIEC"
     gene            complement(4271..4558)
                     /locus_tag="QF838_10475"
     CDS             complement(4271..4558)
                     /locus_tag="QF838_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61142.1"
                     /translation="MAISLTVELYGNSRIIAGQKFIQLKFAESKVGAADVIKELALLF
                     PSLDGKVLDLGGKKLLSSYVFNLNGVGFQGPQKLKLRDGDSLLILPSQAGG"
     gene            complement(4548..5174)
                     /locus_tag="QF838_10480"
     CDS             complement(4548..5174)
                     /locus_tag="QF838_10480"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HJO61143.1"
                     /translation="MEAIELTGILLAGGRSRRLGRDKAVERIGSERIIDRVIQTLAGV
                     TTSQTVVVDRLNRIKELNIEKEIRYVVDSYSETGSLGGLFSGLSQSPTPWSFLTACDM
                     PFLSATLIKFMMTKISNHSPDVVIPKFEGRLQTTHALYNKSCLKHIEARLEAGLLKMD
                     GYFSKITVCEITEDEFLNIEGSGDSFFNVNTPDDLETALRMEKYFNGD"
     gene            complement(5180..7126)
                     /locus_tag="QF838_10485"
     CDS             complement(5180..7126)
                     /locus_tag="QF838_10485"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HJO61144.1"
                     /translation="MSPQAGFERNYFLKDIPLQIAHDKYQEHLLDIGPILKRDTEIIS
                     IKDSVGRVSTQPVFAKISSPNVTTSAMDGVAIRYESSLGATETRPIKLMEGSDFRWVD
                     TGDPVPEGFDSVVMIEDLEKYSATEILIRNPVAPYQHVRKIAEDIAAPELLLQQGAII
                     RSLDLACLGAAGVSDIQVNIPPKVCFIPTGTELVKIGETPGPGQIIEFNSVFLSALVE
                     GWGGKASVQNPVSDDPILLEQALLNSIADCDVLVVIAGSSAGSEDFTSSVISKLGEVV
                     VHGVAIRPGHPVILGSVSGKPVIGMPGYPSSALTTAELFLKPIIYDFLNSNEIIPDQI
                     KSVMGKKVASPLGEEEFLRVLVAPVNGHYIANPTTRGAAMTLSISKSDGVIRIPANSE
                     GLEEGSTVSVILNRQRSEIDKTLLISGSHDIALDILASELTFAENGVRLISSSVGSVG
                     GLLALSKGYAHMAGSHLLDEETGEYNFSAVKRHMPNDKVRIYDFVGRTQGLMVQKGNP
                     KEITDVKDLIVKGTKFINRQRGSGTRLLFDYLLKQSDLDPAKIIGYENEEYTHWNVAA
                     AVSSGFVDAGVGIMAAAVASDLEFIPLENERFQIVVPSKFIGSVKTVDILLEHLLSSD
                     LRNQIASLGGYDVSLMGNVAEEVG"
     gene            complement(7142..8371)
                     /gene="glp"
                     /locus_tag="QF838_10490"
     CDS             complement(7142..8371)
                     /gene="glp"
                     /locus_tag="QF838_10490"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HJO61145.1"
                     /translation="MADFFNVISPPEAIRLMSEKVSPVGFETVDTEDAIGRTLAKRVI
                     SEENSPSFNRSSMDGYSVRASDTYGASDSLPAYLELVGEIPMGSEAVLPLSPGEAAIA
                     YTGGMLANNADAVVMVEHTKITPTGLLEVYRPVAAGENVIMAGEDFHIGESILESGKT
                     IGHAELGSLMSLGITSIQAYRTPKVAVISSGDELVPPKEKLAKGMIRDINLYTIGALI
                     EGLGASPTFFPRVPDEFDELLEVGKKAMELSDMIVFTSGSSISSRDLTAQVINKMGSP
                     GVLIHGLSVKPGKPTILGFSAGKPVIGLPGNPVSAITIFKAVVDPLLRLISGKKIVRS
                     FKTVEAILNSNLSSTSGRTDYVRVQLKNKNGETFAVPVHGKSNLISTILDSDGYVEID
                     SESSGVYKGALLEVYLD"
     gene            complement(8423..10060)
                     /locus_tag="QF838_10495"
     CDS             complement(8423..10060)
                     /locus_tag="QF838_10495"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HJO61146.1"
                     /translation="MSQGWAMKLIENIASDLGILRDDLVPYGSYKAKLSLSAIKKERK
                     GKLVVVTGITPTPAGEGKTTTVVGLAQAMGKMKKNVVATLREPSLGPIFGIKGGGTGG
                     GASKVEPEDEVNIHFTGDAHAVGSAHNLLVALTDNVAQRNKIKGFSPQGVTIRRVTDV
                     EERSLRSVLTGLGGKANAPLRETGFDIVTASEVMAILALSSSLEDLRERLSKIVVGYK
                     DDGTPVQAAEIKAVGSMMSLLRYAIQPNIVQTSEGQPVFVHTGPFGNIAHGCCSVVSD
                     QLALGYADYVLTEAGFGADLGFEKFMHIKARLNDLEPAAAVIVASVRALKSHGGVPLR
                     SLDAANKEALVKGMSNLKHLIGIIKSFNLPVVVAVNSFPTDGSEETELVKSLSIEAGA
                     EHAVVSKVYEDGGEGGLDLADAVIKAADDSPNAISYMYELSDSLEDKISSLATKVYNA
                     TGVNYTPVARRALRQFEENGWGGFPICMAKTHLSLSHNRSLKGLPSDYDFRVSDARAS
                     VGAGFIYPIAGSIMTMPGLPGEPRSLDVDPSGNILGL"
     gene            complement(10116..11351)
                     /locus_tag="QF838_10500"
     CDS             complement(10116..11351)
                     /locus_tag="QF838_10500"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJO61147.1"
                     /translation="MSFSGTYESLKNRDFFFLWVGMLTLMFGMNMQMLVRSYFVYELE
                     SSGTILGIVNAGSAIPMLTLALFGGVIADRLNKKRIIQSGQLLAGCVSLVVAVLILSE
                     AVTWVHLLIASIVQGGVFAFMMPARQALIPQMVGQNLVSNAVALNAAAMSVSTLAAPT
                     AAGLLYEFIGPGKVYLLITVLNFSSIIFTMLIKNKGYSVAPKNGSASDSNRPSVLISD
                     MLQGLKYVRNNSFILILLAIALSTTILAQPFRFIMPIFIVDIYGLKADSMGLLVSVMG
                     LGALMGSIYIASLGNRLRGKVLLISSYFSGFSLLLIAAVPLYLFAALIMLPLGLGDAG
                     RRSINMALIMEEAEEEYRGRVMSIYMLNWGLMPLGVLPAGLMADWLGGQGSIAVLSVL
                     LILFTVWITFKYPEIRRYQ"
     gene            complement(11628..12407)
                     /locus_tag="QF838_10505"
     CDS             complement(11628..12407)
                     /locus_tag="QF838_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HJO61148.1"
                     /translation="MIRNHVRSNLLSGKPSIGPFIGLQSPNVAELMGNAGFDFVVVES
                     EHNGIESAEIEHMLMAIGNTNALPIVRIPSSQQVYIQKALDLGASGIVVPAVKTAQEA
                     AEIVSATRFPPDGKRSWGPLRASKYTFDNQDYLKNANDNTLVVLIIETVEAVGNLQEI
                     AAVPGIDVLFLGPWDMCLSLGLDPLLLPHEEIDQILEKMIEASAKFDVVAGAGASEPD
                     DVCKRLNQGVKFLSYGPDYALLSAAAISGVDAFKNWNKDVD"
     gene            12551..13633
                     /gene="aroC"
                     /locus_tag="QF838_10510"
     CDS             12551..13633
                     /gene="aroC"
                     /locus_tag="QF838_10510"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HJO61149.1"
                     /translation="MSNSFGKAFRITTWGESHGDAVGVVIDGCPPGLPISLEDIQKEL
                     DRRRPGQSEISTQRKEGDKAEILSGVFEGLSLGTPISIAVWNTDAKGKDYEHMKSIYR
                     PSHADYTYQAKYGIRNWKGGGRASARETIGRVAAGAIAKKFLFAEYGTEIVAFVKRIS
                     DIESNIGHEEITLEGIESNMARCPDQDAAVAMIEAVKTARKNGDSLGGIVEAFATNVP
                     VGIGEPVFDKLDADLAKAMLSLPACKGFEVGSGFGGTYSTGSQHNDPFYMSKGTVRTK
                     TNNSGGIQGGISNGESIILRAAFKPTATIMSSQDTVDIFGNDVELQGRGRHDPCVLPR
                     AVPIVESMFALVLCDHVIRHRGQTGK"
     gene            complement(13675..14466)
                     /locus_tag="QF838_10515"
     CDS             complement(13675..14466)
                     /locus_tag="QF838_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJO61150.1"
                     /translation="MDLGLKGKVAIVTGGSDGIGKAAAISMAAEGAKVAIVGRTQEKL
                     NAAVSEIKNTSGGDVIGVAADVSVESDVKAMVDKVVGEFGQLDILVNNAGTSSATTLE
                     KMTDEDLKVDFGIKVYGAVYCARAALPHLKKSGAGAIVNTTTPGGKAPNPGGQPTSLS
                     RAAGISLTKSWAGELAKDNIRVNTVCVGVLKSGQHRARWESANAKDSSYTLEDHWKIV
                     GKNVPMGRIGEAHEAGDLICFLASAKASYITGTAVNVDGGTSPVV"
BASE COUNT         4179 a         3547 c         2763 g         4105 t
ORIGIN      
        1 ctatagcccc aataaactga tggcctgttt ggcatgcctg ggcacaccta ccacaaagga
       61 tgcaagagtc cattgctata ttaaatattg ggttgctagc atctgtttcc tctcgagacc
      121 gagatttcca cacctgacca tttattgaat tggattccat cactctagac aattctctgt
      181 aatcgtcttt atctgaatcg accataccag cagtcaattc cagaacactc tttcttatca
      241 aatccagctt gttaccggtc gtcgaaatct tcataccctg caagataggg gtcgaacacg
      301 acgctggata accacgaact ccctcaatct ccactaaaca ggttcggcaa gcaccgatag
      361 gtttcatatc aggatcttta cataactgag aaatcgccgt cccggaaatg ttgatagaat
      421 caaggacaga actatgttct ggcacctgaa tttctttgcc atttataaaa acttccatta
      481 aattcctcct ctttgggatc ccggcgatac ttcctgaaaa taggagccat agctcagcac
      541 tggattgcca gcggcttggc ctaatccaca gagagaggca gcatttataa cttcagccag
      601 ctggccaatt ccatcccaat taattgggtc atcaccagaa tcatttgcca tcaactcgag
      661 taaacggcta gtgccctctc ggcaaggtgt gcattttccg catgattcct tcgcattgta
      721 ttcagaaagc ttcctgacca catccagcac attagtatct tgagataggc ctatcacacc
      781 ccctgcgccc aacatcactg atttgccatc tataaaacct gggccaattg ataagttgag
      841 ggattcagaa gagggtataa ccccacttga aggcccacct acggcaatgg ctgatagcgc
      901 atttatacta gctatacccc caaatttaac catcaactca gcaaggtttg aacccattgg
      961 taactctgca acaccgggag tgctcaccga tccgctgata ctcatgattt tcgttcctgg
     1021 agtatccaaa tctcctattt ttttaaactc gtcaggtcca ttaagcacaa tccaaggtag
     1081 attacacaaa gtttcaacat tgttaatgat tgtaggctgt tggtttaaac cagattcggt
     1141 cggaaacggt ggtctcaaac gtggttcccg tcttttaccc tctatagtat tgagtagggt
     1201 ggattcctca ccacagacat atccaccagc accttttaaa atttctatat caatggtgaa
     1261 cctactgccc attatatttg gccccaagaa accctttgaa cgacatattt ccaatgcgtt
     1321 tagcatcctc tgataagaca ggtgagcctc ggcattcaca tacaacctaa tggaagtagc
     1381 ttcagtagaa tatgctgcaa tcaaggcccc ttctataaga cgaaatggaa cgctttccat
     1441 gacgtgccga tctttaaata ctccgggctc gccctcttct gcattgatta ctaaatgtct
     1501 ttctttggag acagtggata ttgcggagcg ccatttaatc gaaactggaa aatatgcccc
     1561 acctctacct ctgagaccgg attcttccac catatccacc gtttgttcag gcggttggga
     1621 aagaaccttg gcaagagcag aaaaaccctt attagttata tatccataga tgtcacaggc
     1681 acttatatta cctgcatgct ccaaaactat tcttgtctgg gaattgatgt aattactgac
     1741 atttgaatcg cccgtactcg tcactctaac cccttctaga tattcactca cgtctttttc
     1801 agaatgaatc ttgggaccgg caaagcaggc cccatcgcaa cctgcgtaat caacggcaat
     1861 tgaggcaggc aatctttctt ctaaggtcct gtaaatttct tgggctccaa cagctatgct
     1921 acattgggca acttgaacca gtatttcggc tggattcttt acagctctat tggctacgga
     1981 ggttataaaa tcttgagaat tcattttaat tttctgtcag cctcagtaaa acatcaccgg
     2041 gaacctgtct cccaaaccac ctgtgattca ctgataccgc gggtgcaacc gaacacaaaa
     2101 atgcacaatc cgtcttctgc actttcaaat cagagttagt tttgttagat aaaaattcca
     2161 gtatctgagt tgctccattt tcacgacaag ctacaccatc acataccgac actgtttttt
     2221 gcgcaggggg ggagagcaac aattctgtgt acgtagttgc agtgccatat acttcgctag
     2281 cagggacacc taaatgccat cctaatactt ctagtcccca atcaggcaga taagcaaatt
     2341 tttcctgaag aaagtgcaaa gcaggaagga gatacgatct atcccgtgga aacttatcta
     2401 ttaattccct gcgtaaatcg tttcttcttt ttctttcctc attatttgaa gacatattgc
     2461 tgcggttcag cattctatac ccaaactttt caatttcgat tccggtataa ggccatcgct
     2521 tgtccattcc ctagcatcat agtaggactt aatcataata cccaaatcag attcactaag
     2581 tgactgtctg ttctcatcat ctgatagagg ttcagtaaat atccttttgg gtaagttatc
     2641 ctgatcctta acccatcctt ctcggatgtt aaactgcttt ttcagattat ttattctttc
     2701 accgcaccgg gcaagctgat ttgggtccat atcccaccct gttacgttat tgagtatttc
     2761 agaagattca acataaaatt catcaaatac ctttcgaacg aatttacacc aaattaggga
     2821 atccataacc gcactataat cttctccttc tacaaccatc cggccttttg aagatttacc
     2881 accattggag cttgatgcaa aatcatactc ataggcagaa gatcgattgt ggcacgcacc
     2941 tctggtactt acagccaaag ctagggccat cgtatgcaat gatctaggct catatccagg
     3001 catttcaaga cctttgacat gcatggcaaa atcttctgtc ccaaggccaa tttctttact
     3061 tgccctagca ctaccttctg ccaatatatc cccgatacct gatcgttcag atatagcatt
     3121 caacaatttg gtaagaaggt cttcgtcatt ttcatatata ggcaggagtt ccaaccacct
     3181 tttaggaatc aacccttttt gcatagcctc cataaaccaa gcacatgtgg atcctgcact
     3241 gatggtatcc attcccattt catcacaaaa tttgctgctt ttaagtatcg tctctctgtc
     3301 agatataccg acaagaggtc ccaaggcaaa tagtgattga tattccaatc gcacttttgc
     3361 tgaaccaccg tttggtatgg tcaccaattt ttcacaacct attgtgcaat tagcgcaatg
     3421 cgaagtggaa actttttcct cattatgaat tgcctcacca gatatgctct gaaagtcttt
     3481 atttgattgg ttcctgaaat tcccaatggg aagaactttc aatcgctcga acaccgatag
     3541 gttagccatc gtccctaagg tcctgtattt ttcagtagcc tcccccaagc tttttatggc
     3601 tagctgcctt ctggtttcgg aaaggctcaa ttcattaaaa taattcaatt tcttgtatcc
     3661 cttaaaggat atggctttta ttttcttgga tcccatcacg gccccgtttc cggtccggcc
     3721 tgcctgtcgt cctccatcat tacttatgct ggcaaacctc accatatttt ctcccgccgg
     3781 gcctatgcaa gcggtaccat aacctgggcc taaagtttct attagatggc cctctgttgt
     3841 ggaagtggta ttccctatga gatgtgaagc gtcgttgaat tggataccag attcatcaac
     3901 cttcagagac aaccattggt tggatgaacc aagaattata acggcatccg ctcctatccc
     3961 tttaatagat aaggccagtg aacttgaaga taaagaatcc cctatgaagc ctgtttgtgg
     4021 tgatttggaa accactgcga acttcgaggt tgtggtgaga cgactcccca caagaggact
     4081 cgcacaaaaa attaatgggt tctccggaga aaacgggtcc gtcctaggag ggcaataatt
     4141 aaaaagaaga taggctccta aaccgctgcc accaataaaa tctttgagaa cctcttcttg
     4201 aaggtcttcc cacctccagg attttgagga gacgtctatt attaaaattt tattgtgata
     4261 accgaacacc ttaccctcct gcttgactag gaagtatcaa tagcgaatca ccatcacgta
     4321 atttcagctt ctgaggaccc tgaaatccaa ccccattcaa attgaaaacg taacttgaca
     4381 aaagtttctt gccaccaagg tccaaaactt taccgtcaag agatgggaaa agtagtgcta
     4441 attccttaat aacatcagca gcaccaactt tagattcggc aaacttcaac tgaataaatt
     4501 tctggccagc aattatccta ctgtttccgt acaactcaac cgtcaaacta atcgccattg
     4561 aaatattttt ccatccgcaa tgcggtctca agatcatcag gtgtatttac attaaaaaaa
     4621 ctatctccag aaccttcaat attcaggaat tcatcctctg ttatctcaca cacggtgatt
     4681 ttcgaaaaat atccgtccat tttcaaaagt ccagcctcca atctcgcctc aatatgctta
     4741 aggcacgatt tattgtataa agcatgcgta gtttgcaatc tcccttcaaa tttagggatg
     4801 acaacatcag gagaatgatt cgagattttg gtcatcataa attttatcaa ggtagctgat
     4861 aaaaacggca tgtcacaagc tgttaaaaat gaccagggag taggggattg actcaaacct
     4921 gaaaataacc ccccaagaga acctgtttcg ctataggaat ctaccacata ccgaatttct
     4981 ttttctatgt taagttcttt gattcggttc aatctatcca ccaccaccgt ctgagatgta
     5041 gtaactcctg ctaaggtttg tatgacccta tcgatgatac gttcactacc gatacgttct
     5101 acggccttat cacgtcctaa gcgcctactc ctaccgccgg ctaataatat cccggttaat
     5161 tcaatagctt ccatttacct taacctacct cctcggcaac atttcccatt agtgaaacgt
     5221 cgtaacctcc aagggaggca atctggtttc gtaaatcact actcaaaagg tgctctaata
     5281 atatatccac ggtcttaacc gaacctataa atttagacgg aactactatc tggaatctct
     5341 cattctccaa aggtatgaat tccagatcac tggccaccgc cgctgccata attcccaccc
     5401 cggcatcaac aaacccggaa gacaccgccg ccgctacatt ccagtgagta tattcctcgt
     5461 tttcgtaacc aatgattttg gccgggtcca aatctgactg ctttaacaaa tagtcaaaaa
     5521 gaaggcgggt tccagatcct ctttgcctat ttataaattt tgtacccttt acaatcaaat
     5581 ctttgacatc tgttatttct tttggattcc ctttctggac catcaaacct tgagtacgac
     5641 cgacaaagtc atagatccgg accttatcgt tgggcatgtg ccgttttact gcagaaaaat
     5701 tatattcacc tgtttcctca tccagtaggt ggctacctgc catatgagcg tagcctttgg
     5761 atagtgccaa caacccacct acacttccta cactagagga aattaatctg acaccgtttt
     5821 ctgcgaaggt taattcgctt gccagtatat ccaaggcaat atcatgactt cccgatataa
     5881 gtagagtttt atctatctca gagcgttgcc tatttaaaat tactgaaact gtagagcctt
     5941 cctcaagacc ctcagaatta gcaggtattc ttattacccc gtcagatttg gatatagaaa
     6001 gagtcatagc ggccccacgg gtggttggat tagctatata gtgtccattt acgggtgcca
     6061 ctagcaccct caaaaattct tcttctccca aaggtgaagc tacctttttt cccataacgg
     6121 attttatctg gtctggaata atttcattgg agttcaaaaa gtcatagatg attggcttta
     6181 aaaatagttc cgctgtagtg agggctgaag agggataccc gggcatgccg attactggtt
     6241 tcccagatac ggaacccaat atgactggat ggccaggcct aatagctaca ccgtgtacaa
     6301 ccacctcacc taatttggat ataacggagg atgtaaaatc ctctgaaccc gctgatgagc
     6361 ccgcaataac tactaaaacg tcacagtcag ctatagagtt aagcaacgcc tgttcgagca
     6421 aaataggatc gtcagataca gggttctgta ctgacgcttt gcccccccaa ccttccacca
     6481 gcgctgatag gaaaacggaa ttaaattcga ttatttggcc cgggcctgga gtttccccga
     6541 tttttaccaa ttcagtacca gttgggataa agcaaacctt aggtggaata ttaacttgga
     6601 tatctgacac gcctgccgcc ccaagacaag ccaagtctaa agatctaatt atggcaccct
     6661 gttgtaataa caactcaggt gcagcaatat cttcggctat ctttctaaca tgctgatatg
     6721 gggctacagg attccgaata agaatttcag tagcagaata tttctctaga tcctcaatca
     6781 taaccacaga atcgaaccct tcaggtacag gatctccagt atcgacccat ctaaaatcgg
     6841 atccctccat tagtttaatt ggcctggttt ccgttgctcc taacgaagat tcgtatctta
     6901 tggcgactcc atccatagca gatgtggtca cattaggcga agatatcttg gcaaaaacag
     6961 gctgagtgga aactcttcca acagaatctt tgatggagat tatttcggta tctcttttca
     7021 aaatgggacc gatatccaat aaatgctctt ggtatttatc atgggcaatc tgaagtggaa
     7081 tatctttcag gaaataattt ctttcaaaac cggcttgtgg cgacattgtt agcctttata
     7141 attaatctaa ataaacctct agcagtgcac ctttatacac acctgaagat tcagagtcga
     7201 tttcaacgta tccatcagaa tccaatatag tggaaattag attggatttt ccatgcacgg
     7261 gtacagcgaa agtttcacca tttttatttt ttaactgaac cctgacataa tctgtccggc
     7321 cactagttga agatagatta gaattcagaa ttgcctcaac agttttgaag ctgcgaacta
     7381 tttttttacc agatattagc cttaacaaag ggtctacaac cgccttgaat atagtaattg
     7441 cagacacagg gttaccgggc agaccgatta caggctttcc ggccgaaaac cctaaaattg
     7501 taggtttacc tggttttaca gataagccat gtatcaatac tccaggggag cccattttat
     7561 taatcacttg tgctgtgaga tctctagaac ttattgaact acccgatgta aaaacaatca
     7621 tgtcagagag ttccattgcc tttttaccaa cttccaataa ctcatcaaat tcatctggaa
     7681 ccctaggaaa aaaggtaggt gaggccccaa gcccttcaat aagagcccca attgtgtaaa
     7741 ggttaatatc ccgtatcatg cctttcgcta acttttcttt gggtggaact aattcatccc
     7801 ctgaagatat gactgctact ttcggcgtgc gataggcttg gatagaggtt atccctaaag
     7861 acattaacga tcccaattcc gcatgaccaa ttgtttttcc agattcgagt atcgactcac
     7921 caatatggaa atcttcccca gccataataa cgttctctcc cgccgctacg ggtctataaa
     7981 cttccagaag gccggtaggt gttatttttg tgtgttccac catcaccact gcgtcagcat
     8041 tattcgccaa catcccccct gtataggcga tggcagcctc accaggggac aagggtaaaa
     8101 ctgcctcgct gcccataggt atttctccca ctaattcaag atacgcggga agtgagtcag
     8161 atgctccata agtatcagaa gctcttactg aatagccatc catagaagat ctgttaaagg
     8221 atggggaatt ttcctcacta ataactcgtt ttgcaagtgt tctaccaata gcatcttcag
     8281 tgtccactgt ctcaaatcca acaggagata ccttttcaga catcagtcgt atcgcctctg
     8341 gaggtgaaat cacgttgaag aaatcagcca tcaaaacctc gtctaaccag gctattaaac
     8401 catggccaac ccagattaaa ttctaaagtc ccagaatatt accagaggga tccacatcta
     8461 gtgacctagg ttctccaggc aatcctggca ttgtcatgat acttccagct atggggtata
     8521 tgaatcctgc accaacagat gctctggcat cagacaccct aaagtcatag tcagaaggaa
     8581 ggcctttcaa tgatcggttg tgtgaaagcg acaagtgtgt ttttgccata catattggaa
     8641 agccacccca tccattttct tcaaattgtc ttaaggctcg tctagccacg ggagtataat
     8701 tgaccccagt tgcgttataa actttggtag ctaaagatga aattttgtct tctaaggaat
     8761 cagacaattc atacatatat gaaattgcat tcggagagtc gtcggcggct ttaataacag
     8821 catcagcaag gtcaaggccg ccctctcctc cgtcttcata aactttcgat acaacagcat
     8881 gctctgcacc agcttctata gaaagggatt tgacgagttc tgtctcttca gagccatccg
     8941 tgggaaaact atttaccgct acaactacgg gtagattaaa gctcttaatg attccaatta
     9001 gatgtttaag attagacatg cctttaacca gagcttcttt gttagccgca tccaaactac
     9061 gaagtggaac tccgccatgt gatttaaggg cccttacaga tgccacaatg actgcggcgg
     9121 caggttcaag atcatttaac cgtgccttta tatgcatgaa tttctcaaat cccaaatcag
     9181 ctccaaaacc tgcttcggtt aagacataat cagcataacc aagtgctaat tggtctgata
     9241 ctaccgaaca acatccatga gctatattcc caaacggacc agtatgaacg aaaaccggct
     9301 gcccttcgga tgtttgtact atgttcggtt ggatagcata ccgcagcaac gacatcatgg
     9361 acccaacagc ctttatctct gccgcttgta ccggggtgcc gtcgtcttta tatccaacga
     9421 ctattttgct caatctttcc ctgagatctt ccaaacttga tgacaaggct aaaattgcca
     9481 tcacctcaga ggctgtgaca atgtcaaatc ctgtctcacg gagaggagca tttgctttcc
     9541 caccgagacc tgtcagcacg cttctaagag acctctcttc cacgtctgtg actcttctaa
     9601 tagtgacacc ttgcggtgag aatcctttaa ttttatttct ttgggcgacg ttatcagtca
     9661 aagcaaccag taaattgtga gccgacccta cggcatgtgc gtcaccagtg aaatggatat
     9721 tcacttcatc ttctggctca actttggaag caccaccacc tgttcctcct ccctttattc
     9781 caaatatagg tcccagagag ggctctcgca atgtagcaac aacatttttt ttcatcttac
     9841 ccatcgcttg cgctaaacca acgacggtgg tggtttttcc ttctccagcc ggagtggggg
     9901 taataccagt aaccaccacc agcttccctt ttcgttcttt cttaattgca gacagagaca
     9961 attttgcctt ataggaacca taaggaacta agtcatcccg caagattcct aagtcactag
    10021 caatattttc tattaacttc atagcccatc cctgagaaat ctattaacac tttccacaat
    10081 tggtaaggat atcatcgaat atcacatttt atcgtttatt ggtaccgtct aatctccggg
    10141 tatttaaaag ttatccacac cgtgaaaagt attaatagaa ccgataacac tgcaattgag
    10201 ccctgcccac ctaaccaatc tgccataagc cctgctggca gtactcccaa aggcattaag
    10261 ccccaattca gcatatatat actcatcact cgcccacggt attcttcctc agcctcctcc
    10321 attatcaggg ccatgttaat ggaccgacga cctgcatcgc ctaggcctaa aggaagcatt
    10381 attaatgcag caaataaata caaaggtacc gcggcaatca agagcagact aaatcctgaa
    10441 aaatacgaac tgataagtag cactttccca cgcaacctgt tgccaaggga ggctatataa
    10501 atagatccca ttagggcccc gagccccata acagaaacca gtagccccat tgaatctgct
    10561 ttcaacccat atatatctac tatgaaaatt ggcattataa atctaaaggg ttgagctaaa
    10621 atagtcgtag ataaagcaat tgcgagaagt atcaaaatga aggaattgtt acggacatat
    10681 tttaatccct gaagcatatc gctaattaga accgaagggc gattggaatc ggatgctgac
    10741 ccattctttg gggccacact gtatcctttg ttttttatca gcatagtgaa tatgatcgaa
    10801 gagaaattga gaactgttat caacaagtaa actttgccag gaccaatgaa ttcgtaaagt
    10861 aaaccggcag cggtaggtgc ggctaaagtg ctgacactca tcgcggcagc attgagggcc
    10921 acagcgtttg acaccaaatt ctgtcctacc atctgcggta tcagcgcctg acgggccggc
    10981 atcataaagg cgaacactcc gccttggaca atcgatgcta taagcagatg aacccaagtg
    11041 acggcctcac ttaaaattaa gactgccact acaagtgaca cacaccctgc caacagttgt
    11101 ccgctttgaa ttatcctttt cttgttaagc ctgtcggcaa taactccgcc aaacaaagcc
    11161 aaagtcagca taggaattgc tgatcccgca ttgactattc caagaatagt ccctgaactc
    11221 tcaagttcat acacgaaata actccgaaca agcatctgca tattcatacc gaacatgaga
    11281 gtcagcattc ctacccataa aaagaaaaaa tcgcgattct ttaaagactc gtaagtacct
    11341 gaaaagctca ctgttgaact tgcaacaaag aaaatctaat taacaaaaat tgaaccaatc
    11401 ctgatctcta aaatttctcc aagaccctag ttaaaggggg tccgaaccac ggtagactct
    11461 cattcaaaag aaaggtcacc gcaggtgttc agttcggacc cgtttgaact catttatggt
    11521 acaaatattt gagtaaccac aacatatccc cctaccacta tcgattctaa cactatcgtt
    11581 atccctgcaa tcagactatt tccccctgca gtaaccaatc ccttccctta atcaacatcc
    11641 ttattccagt ttttaaatgc atctactcca gaaattgccg ctgcagataa cagggcatag
    11701 tcagggccat agctcagaaa tttgactcct tgatttagtc ttttgcatac atcatctggt
    11761 tcagacgcac ccgcccctgc gaccacatca aacttagccg aagcttcaat cattttttcc
    11821 aatatctggt cgatttcctc atgaggcaac aaaagagggt ccagtccgag ggataaacac
    11881 atatcccatg ggccaagaaa taacacatca atcccaggaa ctgcagctat ctcttgaagg
    11941 ttaccaacag cctctacagt ttcgattatt aacaccacaa gagtgttatc gtttgcattt
    12001 ttcaaataat cttgattgtc aaaggtatat ttagatgctc tcaacggtcc ccaacttctc
    12061 tttccgtccg gtggaaacct tgtggcggaa acaatttcag cagcctcctg tgcagttttt
    12121 accgcaggaa caacgatccc cgatgcacct aaatctaagg ctttctgtat gtaaacctgt
    12181 tgcgaggaag gtatcctgac aatcggcaag gcattggtgt ttcctattgc cattaacatg
    12241 tgttcaattt cggccgattc tattccatta tgttctgatt ccactactac gaagtcaaac
    12301 cctgcattgc ccatcaattc agctacgtta ggactttgaa gaccaataaa aggtccaatc
    12361 gaaggtttcc cagacaataa gttacttcta acgtgatttc ttatcatatc catacacctt
    12421 attagctacg tatatcaggt ttattgagtt ttgtcccaaa aatccagttg aatacgggat
    12481 tataatgttt cctacacatt taaataaata gcataacaat tagtaacctt gattaggtta
    12541 acgacaaaaa atgagtaatt cattcggcaa agcattcaga ataacaacct ggggagaatc
    12601 gcacggcgat gctgtggggg tggtaattga tggatgtccg ccaggcctac ctataagttt
    12661 ggaggatatt caaaaggagc tcgaccgacg aagaccgggg caaagtgaaa tatccactca
    12721 acggaaagaa ggtgataaag ctgagatctt atcgggagta tttgaaggat tatctttagg
    12781 aaccccaata tcaattgcag tgtggaatac tgacgcgaag ggcaaagatt acgagcatat
    12841 gaaaagcatt taccgacctt cgcatgctga ttatacctat caagccaagt acggaatcag
    12901 gaattggaaa ggtggcggga gagcctctgc cagagaaact atagggcgag ttgctgcggg
    12961 agcgatagca aagaagtttt tatttgcgga atatggcact gaaattgttg ccttcgtcaa
    13021 gagaataagt gacatagagt caaacattgg acacgaagag ataaccttag agggaattga
    13081 aagtaacatg gcgagatgcc ctgaccaaga tgctgcggtc gcaatgatag aagcagtcaa
    13141 aacggctagg aagaacggtg attcattggg ggggattgtg gaagctttcg cgacaaatgt
    13201 gccggttgga attggagaac ctgtgttcga caaattagac gccgacctag cgaaagcgat
    13261 gctatcttta cctgcatgta aagggtttga ggtcggatcc ggttttggcg gaacctactc
    13321 aacaggctcc caacacaatg atcctttcta catgtcgaaa ggtacagttc gaaccaaaac
    13381 taacaactct ggtggcattc aaggtggaat tagtaacggg gaatccatca ttttgagagc
    13441 cgcattcaag ccaacggcga cgattatgag ttctcaagat accgtggata tttttggtaa
    13501 tgatgtcgag ttgcagggta gagggcgcca cgatccatgc gtattaccaa gggcagtacc
    13561 aatcgtggaa tctatgttcg ccctagtatt atgtgatcac gttattcgcc atagggggca
    13621 aacaggaaaa taaggggagg gagttttaat ctccctcgcc tttaaaaagt atttttagac
    13681 cacgggtgat gtgccaccgt caacgttaac cgcagttcca gtgatgtagc ttgccttagc
    13741 tgaagctaag aagcagatca aatctccagc ctcgtgagcc tcaccaatgc gacccatcgg
    13801 aacattcttt ccaactattt tccaatgatc ttccaacgta tagctagaat cttttgcatt
    13861 agctgattcc catcttgcgc ggtgctggcc agatttcaat actcccacgc agacagtatt
    13921 tactctgata ttgtcttttg ccaattctcc tgcccacgat tttgtgagtg aaatacctgc
    13981 tgctctagac aatgaggtag gttgcccacc aggattagga gccttccctc cgggggttgt
    14041 cgtatttaca attgccccag ccccagattt tttcaaatgg ggcagggcag cccgtgcaca
    14101 ataaacagcc ccataaactt ttataccgaa atcgactttt aggtcttcat cagtcatttt
    14161 ttccagggtt gtagcagaag aggttcctgc gttattaacc aaaatgtcta gctgcccaaa
    14221 ttctcctact accttgtcca ccatcgcttt aacgtcagat tcaaccgaca catctgctgc
    14281 aacaccgata acatccccac cggaggtatt tttgatttct gaaactgccg cattcaattt
    14341 ttcttgagtt cggccgacaa tagcaacttt agctccttct gcagccatgg atatagcagc
    14401 ggcttttcca attccgtcac tacccccagt aacgattgcg acttttcctt ttaaccctag
    14461 atccatacct tttctccttg ttttttattt tttttattaa ttacttttat attttttgga
    14521 aggttattca atacttctaa tgtctcagag tcatctgatc catcattaac tacaattatc
    14581 tcaatatctt tata
//
LOCUS       DASCNS010000299         1845 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002142119, whole genome shotgun sequence.
ACCESSION   DASCNS010000299 DASCNS010000000
VERSION     DASCNS010000299.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002142119"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(235..393)
                     /locus_tag="QF838_10520"
     CDS             complement(235..393)
                     /locus_tag="QF838_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJO61151.1"
                     /translation="MPATMILGCTVEKDDSISLIHKAIDDGITFVATAKPYNNGLAEE
                     VVGMALVR"
     gene            complement(440..1459)
                     /locus_tag="QF838_10525"
     CDS             complement(440..1459)
                     /locus_tag="QF838_10525"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HJO61152.1"
                     /translation="MIIRQKQEPLLYERTLGMSVMEGKGFYYPNDAAVSSNGKIYVLN
                     RSLEARGSGRGMRVTICDSSDEFHGDFGEFGNKPGQFMWPSAITQGPDQNIYITDEHL
                     HKIMKFTAAGLFLKEWGQFGSEPGNLDAPSGIAFNSTGELFVSDTYNNRIQVFTPSGE
                     YIRCLDNNGNLNLPWRLSFDSSDYLYVADWGNDRICKYDPDGKFMSSFGKTGGGEGEF
                     LRPADVVVDDAGRMFVCDWGNERIQVIDANGQFLQMNLGQSDLSPWATNFLNINVEEG
                     KAREKSDLHKKDIKFANPHDRHEISSHIEEYFWSPMALSLTEDGHLYVIESNRHRIQI
                     FKTVT"
     gene            complement(1481..>1845)
                     /locus_tag="QF838_10530"
     CDS             complement(1481..>1845)
                     /locus_tag="QF838_10530"
                     /inference="COORDINATES: protein motif:HMM:NF020390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HJO61153.1"
                     /translation="TMKMTFMGQHLTNPPSVEGWHEGEEWIETGALVERVNFAAEQLG
                     NLKMPGVTAMVDNVLSKAASGQNIPELCLLQMGNLEVEDSIKLILQETVEKSEEPDRS
                     TIEDLFRIIAASPEYQRC"
BASE COUNT          501 a          424 c          358 g          562 t
ORIGIN      
        1 actatcaaca acatcaactt ggtgataatg aagtaagtgc tgacctagaa aaatagacgc
       61 cccttgcatt gagcaggggg cgtttctttg tagattcagg agagccaacc ctctcaactt
      121 aaatcccatc gcatagaaat tgctaataaa tacagaaatg gttggcaggg agaccctgta
      181 ctcctcactg tgttctcgac gaacctggct aagagatagc ctagatcgtt ctttttaccg
      241 aacgagtgcc atacctacta cctcttctgc taatccgttg ttatagggtt tagccgtggc
      301 aacaaaagtg atcccatcat ctatggcttt atggattaaa gaaatagaat cgtccttttc
      361 aacagtgcag cccaaaatca tggttgccgg catagggagg atacttttaa tcctgatttt
      421 cgcaggttac ctaattacct taagttactg ttttaaaaat ttgtatccta tgtcggttac
      481 tttcaataac gtaaaggtgt ccatcttcag tcagtgataa tgccattggg gaccagaaat
      541 attcctcgat atgtgatgag atttcatgcc tatcatgtgg gttggcaaat tttatatcct
      601 tcttatgaag atcggatttt tcccttgcct taccctcctc aacattaata tttaagaaat
      661 tggtcgccca tggagaaagg tctgattgac ctagattcat ttggaggaat tgtccattgg
      721 catcgattac ttggatcctt tcattgcccc aatcacatac aaacatcctt ccagcatcgt
      781 caaccaccac atcagcgggg cgtaaaaatt ccccttcacc gccaccagtt ttcccaaatg
      841 atgacataaa ttttccatct ggatcgtatt tacatattct gtcattcccc caatccgcga
      901 catataggta atctgaggaa tcaaaactta aacgccatgg aagattgagg tttccgttat
      961 tatctaagca gcggatatat tctccggaag gagtgaaaac ttgaatgcgg ttgttatagg
     1021 tatcagatac aaaaagttcc cctgtgctat taaatgcaat tccggacgga gcatctaaat
     1081 tcccgggttc cgacccaaat tgtccccact ctttgaggaa aaggcccgct gctgtgaact
     1141 tcattatctt atggaggtgc tcatctgtga tgtagatatt ttgatctgga ccttgtgtaa
     1201 ttgccgatgg ccacataaat tgacctggtt tatttccaaa ttcaccaaaa tcaccatgaa
     1261 attcgtcaga ggaatcacag attgttaccc tcatgccacg tccactgcct cgggcttcca
     1321 gtgagcgatt gaggacataa atttttccat tcgaagaaac cgccgcatcg tttggataat
     1381 aaaagccttt accttccata acagacatac caagggttct ttcatagagc aatggttctt
     1441 gtttttgtct tataatcatt tcaacgactc ctaaaaaaat ctagcacctt tgatattcag
     1501 gggatgcggc tatgattctg aataagtctt ctatggtaga tcgatcaggc tcttcacttt
     1561 tttccacagt ctcttgcaag atgagcttga ttgagtcttc aacctccagg ttgcccattt
     1621 ggagaaggca taattcaggg atgttttggc cacttgctgc cttacttagg acattgtcaa
     1681 ccatagcggt tacaccgggc attttgagat ttcccagttg ttccgcggca aagttaaccc
     1741 tttcaacaag ggcaccagtt tcaatccatt cttcaccctc atgccaccct tccacgctgg
     1801 gtgggtttgt caaatgttgt cccataaaag tcatcttcat tgtgt
//
LOCUS       DASCNS010000300         2759 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002162821, whole genome shotgun sequence.
ACCESSION   DASCNS010000300 DASCNS010000000
VERSION     DASCNS010000300.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002162821"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="QF838_10535"
     CDS             complement(<1..589)
                     /locus_tag="QF838_10535"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HJO61154.1"
                     /translation="MAHSIDWPEEYKRGLVVVAHADDAEYGCSGTVARLIAEGWDMAY
                     VLCTDGSKGSSDPDITEEQLSEIRREEQENAGKVLGLSNVAFLGHPDSYLQPTLDLRK
                     DIAREIRRQKPDVVICQYPMRVLDGGFGSGHPDHMTAGDTALSAIFPTARDHMTFPEL
                     IEEGFEPHKVAEAWIMGHPEPDLWVDISDHMETSIK"
     gene            complement(647..2116)
                     /locus_tag="QF838_10540"
     CDS             complement(647..2116)
                     /locus_tag="QF838_10540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HJO61155.1"
                     /translation="MNFHLIYPEFLLIGLAFLVLMLDFFLSEKKKYLLNWVSTLGMAF
                     ILGIALFMNQQGDLYQGLLIIDGYASFFRIFFLAIGIVVTLISRDYVDSNISNPGEYY
                     AILIFTILASISLASSGELLTAYVSLEILSFGLYVLVAYDRYNPASNEGGVKFILLGA
                     FSSALILYGLSQTYGLIGTTRFNEIAVLLNSMENISIGMVVGFVLILAGIAFKLAAIP
                     FHMWAPDAYEGAPIPITAWLSVCSKVAIVALAIRFFTNGIFPLAPDWQPILIVLAVLT
                     MIGGNLLALVQKNMKRLLAYSSIGHAGYLLMGLATLVGVSDEGIVSKELTHLVSNGLI
                     FHMVAYGATNFAIFFCLIIVYNSTGREDIQGLSGLASRQPLVALVMVCSLFSLAGMPV
                     FAGFTSKFYLFNAVASQGFLLLVGVGIIASLISLYYYLTVARYLYIEKISDNSMIYVS
                     FNSKLILIALLMFMILGGVYPSPLMDLIQTATDSILKID"
     gene            complement(2113..>2759)
                     /locus_tag="QF838_10545"
     CDS             complement(2113..>2759)
                     /locus_tag="QF838_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HJO61156.1"
                     /translation="LGAVNVIYGALSAMGQTDLKYVIGYSSVSHMGYVLMGLGTLSLV
                     GLNGAVLQMFSHGVMTALFFAVVGMIYERTRTRDSLVLSGLAQKMGIIAVLFTLAGLT
                     SLGLPGLSGFAAELLIFIGIFQSYEIWGIILGSLAVIGAAITAVYILRLLAKVFFGLA
                     DETLPEYLDSTPREKFAASILVIFIVLVGLWPFPFVKIIESGVEPILLQIVGAG"
BASE COUNT          923 a          652 c          475 g          709 t
ORIGIN      
        1 cttttattga agtctccata tggtcactta tatcaaccca aaggtcaggt tccggatgcc
       61 ccattatcca tgcttctgcg actttatgag gctcaaatcc ttcttcgatc aattcaggaa
      121 aagtcatgtg atctctagca gttggaaata tagcagaaag ggcggtatcg cccgcggtca
      181 tatgatctgg atgcccagaa ccaaaaccac cgtctaggac tctcatggga tattgacaga
      241 tgaccacatc cggcttttgt cgacgaattt ctctagctat gtcctttctt aaatctaagg
      301 taggctgtaa ataactatca ggatgtccta aaaaggcaac gttagataag cctaaaactt
      361 ttcctgcatt ttcttgctct tcccttctaa tctcagataa ttgctcttcc gttatgtcag
      421 ggtcactact gcctttactc ccgtcagtgc acaagacata ggccatatcc caaccctcag
      481 caattaatcg agctactgtg cctgaacaac catattccgc atcatcggca tgagctacta
      541 caacaaggcc tcttttatat tcttcaggcc aatctattga atgagccaat ttcatctcct
      601 ttctctcagt gtaattagaa atccaccgtt cagtctatca gaggaattag tctattttta
      661 agatggaatc ggtggcagtt tgaatcaaat ccatcaaagg tgaaggatag actcccccta
      721 aaatcataaa cattaataat gctataagaa ttaatttact gttaaatgaa acatatatca
      781 ttgaattatc ggagattttt tcaatataca aataccgtgc tacggtcaag taatagtaca
      841 gtgagatcag gcttgcaata atccccaccc caaccaacag taaaaatccc tgggatgcta
      901 cagcattaaa cagataaaat ttactagtga atcctgcaaa cactggcatg cctgctaaag
      961 aaaacagaga gcaaaccatc accaaagcaa ctaacggttg ccttgaagca agtcctgaga
     1021 gtccttgaat atcttctcta ccagtactgt tgtagacaat tatcaggcag aaaaaaatcg
     1081 caaaatttgt cgccccatag gcaaccatat gaaaaatcaa tccgttcgaa actaaatggg
     1141 ttagttcctt acttacaata ccttcgtcgc taaccccgac taatgtcgcc aaacccatca
     1201 gcaaatatcc ggcatgtcca atgctggaat aagctagtaa tcgtttcatg tttttctgca
     1261 caagtgctaa taaattacca cctatcatgg taaggactgc caagactatt aaaatgggct
     1321 gccaatcagg agcaagagga aatataccgt ttgtaaagaa tctaatagcc aaagcaacaa
     1381 tcgctacttt ggaacagacg gacaaccaag ccgttattgg tataggggct ccttcatagg
     1441 cgtccggagc ccacatatga aatggtatgg cagccagttt gaatgctatt ccagccagaa
     1501 tcaatacgaa cccgacgacc ataccaattg atatgttttc cattgagtta agtaaaacag
     1561 caatttcatt aaatctagtg gttcctatca gcccgtatgt ttgactcaag ccatatagaa
     1621 ttaaagcaga ggaaaaagct cctaaaagga taaacttgac acctccttca tttgaagcag
     1681 gattatatcg atcatacgcg actaatacat acaacccgaa gctcaaaatt tctaggctga
     1741 cataggctgt cagtaactcg ccggatgaag ccaacgatat tgatgccaaa atagtgaaaa
     1801 tcaaaatagc gtaatattca ccgggatttg aaatattaga gtccacataa tctcgtgata
     1861 taagtgtcac tactattcct attgctagaa aaaatatcct aaaaaaactc gcatacccgt
     1921 caatgatcaa taatccttgg tataggtcac cttgctgatt cataaacaag gctatcccaa
     1981 gtatgaaagc catacccaag gtggagaccc aattcaacaa atatttcttt ttctccgata
     2041 agaaaaaatc taacatcaga acaaggaacg caagcccaat taaaagaaac tctggatata
     2101 tcaagtgaaa gttcatccag cacccacaat ttgaagaaga atcggctcaa caccactttc
     2161 aattattttc acaaatggaa acggccagag acccactaac acaataaaaa taactaagat
     2221 acttgccgca aatttctccc ttggtgtcga atccaaatat tctggcaaag tttcatcagc
     2281 taggccgaaa aatactttag cgagcaacct caaaatatat acggctgtaa tcgcagctcc
     2341 aataactgcg agggatccca aaatgatgcc ccaaatctca taactttgaa aaataccaat
     2401 aaatatcaat agctctgctg caaatccact aaggcctggt aaaccaagag aagttaaacc
     2461 cgccagggtg aacaagacag ctattattcc catcttctgt gccagtccgc taagtactaa
     2521 agaatccctg gtacgtgtac gttcgtaaat cattcctacc acagcaaaaa acagagcagt
     2581 catgacccca tgggaaaaca tttgcaacac agctccgttc aagcccacaa ggctcaatgt
     2641 gcccagtccc attaatacgt agcccatatg actaactgat gaatagccta tgacatattt
     2701 caggtccgtc tgacccatgg ccgaaagagc accataaatc acattgaccg ccccgagtc
//
LOCUS       DASCNS010000301         8575 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002168878, whole genome shotgun sequence.
ACCESSION   DASCNS010000301 DASCNS010000000
VERSION     DASCNS010000301.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002168878"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(11..886)
                     /locus_tag="QF838_10550"
     CDS             complement(11..886)
                     /locus_tag="QF838_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61157.1"
                     /translation="MTKRVEGKVAIVTGAGRGIGREVALWLARDGAKVVVNDLGGSVD
                     GTGDSATPADLVVDEIKAEGGEAVASYDSVADFHSAAKIVEKAVDTFGTVDILCHPAG
                     ILRDRMIFNMTEEEWDLVLQVHLYGAFNMVRNVVPHMIKNKYGRIVLFSSGSGLGASG
                     QGNYAAAKEGMVGFTRAVSKELEPFNIMVNAVYPGGSTRMTDTVPQSTRQLRQDQREA
                     VGLATADPSEPPPEYRDPGNNAPKVVYLCSEAADGITGQVFGTSGWALSLYSPRHVIK
                     SIHKDGRWTLDELDQ"
     gene            complement(950..1873)
                     /locus_tag="QF838_10555"
     CDS             complement(950..1873)
                     /locus_tag="QF838_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61158.1"
                     /translation="MGNRLEGKVAAVTGGGRGIGRGIAKLLAEEGASILINDKGAEVD
                     GTGASNMPADSVVEEIKSAGGNATANYADISTMEGGENLIQSCIDTYSHIDILVNSAG
                     SLKDRMIYQMTESDFDQVVRNNAKSVFTTTKFAGILFRQQRSGRIVNMVADAGLGDVG
                     RSNYAAATEGIIGLTRTTAKDLGKYGITANAISPMAETRLFPGAVDQHRLPDVSGPSR
                     DERAGIGPSPEIPEWLGEGSPDEPENIAPLAVYLCTNAAPNINGYVLGVRGGSLYVYS
                     NPDIENVVNKWGNFTMEEMDVLFPKMMGYGF"
     gene            complement(2015..3046)
                     /locus_tag="QF838_10560"
     CDS             complement(2015..3046)
                     /locus_tag="QF838_10560"
                     /inference="COORDINATES: protein motif:HMM:NF021615.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HJO61159.1"
                     /translation="MGGCKNCDFENTESSLFCGGCGKEIATQCERCRTINRNQTFCTN
                     CGQVLSHDLGEIYNPGAVDESENEKSKTDIPSPSVARLIPEPGKLVDLISISSYAYRK
                     NFKNFLILSLINGIPVLVIGIILRTALETDWVSLANFQPYNDGQLSENLMSDLKSIPI
                     WKLFSIFILILIGLVANLISVMAIIVGAAQFANSEKVSSIACLNYTLSKIGRLILLSL
                     FLIGLLIIPFLLSIMLIGIPILLYLFVRWYFSLPSMALDNTGITRSIQMSWDLVEKRW
                     WVTFGTGLVIVILMTIPTYLLNLLSPNLNSDFIILLIDVCVTTLVSPFQAIATGLYFL
                     SLKKFPKEQ"
     gene            complement(3050..5701)
                     /locus_tag="QF838_10565"
     CDS             complement(3050..5701)
                     /locus_tag="QF838_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HJO61160.1"
                     /translation="MISIPFQKHTLGNGLDIITHVDKTVPTVAVNIWYHVGSKNEIPG
                     KTGFAHLFEHVMFEGSRNHNKDYFGPLQEIGATVNGSTTNDRTNYWENLPSNYLELAL
                     WLESDRMGFLLEALDQKRFDLQREVVKNERRQSYENRPYGMAYLKLQELLFPPPHPYN
                     WPTIGSQEDLDGAEIEDVKEFFRNFYHPSNASISIAGDIDPNSTFDLIDKYFGNLSPG
                     PAINRFHKMDSILKGQTWVTLEDSVHLPRLYLAWPTVPEFTQEQAALDVLSVIMADGR
                     SSRLEQNLVYKKQLARDIRAYHHGQEISGEFHIIATANPGQTTGEIYKEIQALLDELS
                     QTPPSDKEMERAYNIINSYHVRQLEKIGGFGGKADQLNHFNVMAGNPGLIETDVERYK
                     KVSANDVSAAAKLLNGNFVGLTVNPLKDTKPSSRQLDRTNLPKVAALTTFSAPQPKDI
                     FLENGTRLLVIERSELPLTTVGVFFDTGSAEDLEERPGIAQFATDMLFEGTQSRSSSQ
                     IADEMEFLGSHVTKDVSREHTFLAVSGISDNTNKELDVLSDIILNPNFPVTEMERIRK
                     EKLSDLRSIQDSADATASVAMRSILLGPTTPYGHTINGDETSIAAMKTGDLEKHYHET
                     LLTSKKTFIVVGNISSNLAAELITEKFRTFENRQKPQENQVASKPLAGEGPTIYLVDR
                     PGSAQSILRAGHISIPREHQDYHSLAFVNYILGGDYSSRLNMNLRQDKGYSYGFHSSI
                     EWMKPLSILVTRGSVQTEVTKESVFETLKELTDIVELNPIGLEEFRKAKEGLLKSVPS
                     QFESNQQITNQLLNIAAFDLPLDYFETNIEKISDLTLDDVRQSAVKHILPAEIKMIVV
                     GDKEKIQKNLEELGYPLFVIDMYGNNI"
     gene            complement(5705..6139)
                     /locus_tag="QF838_10570"
     CDS             complement(5705..6139)
                     /locus_tag="QF838_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61161.1"
                     /translation="MKENLYFEDVEIGDDIGPIKKSVTDKDVIEFVSIREKEITPSRF
                     TSKEIANNEGMPEAIVPGPMNIALMSQILTDWSDTVCLLKIDVVFRQTVPHNQDLTFS
                     GIVTDKDETGDSTKLECDVVMENSDGVKLVIGTGVIALPNKP"
     gene            complement(6136..6588)
                     /locus_tag="QF838_10575"
     CDS             complement(6136..6588)
                     /locus_tag="QF838_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024844.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HJO61162.1"
                     /translation="MTTDEGIVYDRSLLGVETQIGTFELTREMLLGFAKSTGETNPRY
                     VGDEHGAGHIVAPPTICNVFVSGVSRPDIKLEFGELGFFAGQSIECKAEIRPGDVLSA
                     STKLDKVYAKTGRSGKMVFAVWETKFSNQDEETVALVTESFVRRNKNR"
     gene            complement(6610..7251)
                     /locus_tag="QF838_10580"
     CDS             complement(6610..7251)
                     /locus_tag="QF838_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024660.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HJO61163.1"
                     /translation="MLLDLHTHSVASDDSRATVEQYIKWVNVLRRKGKSIHGFVLTEH
                     RQFDHDVDYSKLASDDGIIILKGAELDTDTGHFLVYGISEQITNELDFSDVNISAQNL
                     VDVCDTYGGIAIPAHPGRVGIGFAEYLDNGVTGFETVKTVEQLNGSNRPGEGEKAESL
                     IKAKGYLGTGGSDAHIVSAVGTCMTRFEKKIANESDLVQELRNGRFTAVKLES"
     gene            7362..8138
                     /locus_tag="QF838_10585"
     CDS             7362..8138
                     /locus_tag="QF838_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61164.1"
                     /translation="MDLGLTGKIALITGGSRGLGRESALSLAREGVSVAICGRTKNVL
                     DATVKEIEQIGATCVGIVADVSDTRAIEVLHSSVIEALGPIDILVNNAGGTRSRDDIN
                     GTPLEDFKGTFDLNVFGAFELMQHVIPSMRSNGWGRIINIASIWGREYGGNISYMSAK
                     AALIAASKHSAISLAKDGILVNSIAPGSIEHPGGSWERFQNDNSPQVVENFIKENLPL
                     GRFGWPEPLGDLVAYLASDRSGLITGSCIVIDGGQSVSMI"
     gene            complement(8319..>8575)
                     /locus_tag="QF838_10590"
     CDS             complement(8319..>8575)
                     /locus_tag="QF838_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014841.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJO61165.1"
                     /translation="VSLEELLENSDFVSLHAPLTPNTEKMIGSEQFDLMRDDAFLINA
                     ARGPLIDEIALYEALSEGKIGGAGLDVMVDNETSRDHPLI"
BASE COUNT         2263 a         1845 c         1714 g         2753 t
ORIGIN      
        1 agatcggtat ctactgatct aactcatcta aagtccatct cccatcttta tgtatgcttt
       61 ttattacgtg ccgtggagaa taaagcgaca atgcccagcc actggtacca aaaacttgtc
      121 ctgtgatacc atccgcggct tcagaacaga ggtagactac ctttggtgca ttgttaccag
      181 gatctctata ttctggaggt ggttcagacg ggtctgcagt tgctaaaccg actgcttctc
      241 tctgatcctg tctcaactgc ctcgtcgatt gagggaccgt atctgtcatt ctagtagacc
      301 ccccaggata gacggcattc accatgatat taaagggttc gagttccttt gacacagccc
      361 ttgtgaatcc aaccatacct tccttggctg cagcataatt accttggcct gaggcaccta
      421 atccggatcc agaggaaaat aatacgattc tgccgtattt gtttttgatc atgtgaggga
      481 ctacattcct taccatattg aaggccccgt acaggtggac ttgtaatact aggtcccatt
      541 cctcctcagt catattaaat atcatcctgt ccctcaatat gcctgctgga tgacaaagta
      601 tgtccactgt cccaaaagtg tctacagctt tttcgacaat tttagcggca ctatgaaaat
      661 ctgctactga gtcatatgac gcaacagcct ctccaccttc ggcctttatt tcatcaacta
      721 ctaaatctgc gggagtcgca gaatcaccgg tgccatcaac tgatccccca aggtcattaa
      781 ctacaacttt ggcaccgtcc cttgctaacc aaagtgcgac ttcccttcct atgcctcggc
      841 cggcccctgt tactattgct acttttcctt cgaccctctt agtcaccttt tcacctcttc
      901 gtttgctttt ttttcatgtt catcaaaatt ggttcggaag aaattgattt cagaatccgt
      961 accccatcat tttcgggaac aaaacatcca tttcttccat tgtgaaattt ccccatttgt
     1021 ttactacatt ttcaatgtct gggttggaat aaacgtacaa gcttcctcca cgtacgccca
     1081 gcacgtaacc attaatattg ggggcagcat ttgtgcatag gtaaacagct agtggggcaa
     1141 tattttcggg ttcatctgga gaaccttctc ccaaccattc gggtatctct ggtgatggtc
     1201 caatccctgc tctttcatcc cgggaaggtc cagatacgtc tggtagtctg tgttgatcta
     1261 cggcacctgg gaataatcta gtttccgcca taggtgatat tgcattggca gtaataccat
     1321 atttgcccaa atcttttgca gtagttctcg ttaatcctat aataccttcg gtcgctgcgg
     1381 catagttcga ccgtcctaca tcaccgaggc ctgcatcagc caccatattt acgattcttc
     1441 ccgacctttg ctgacgaaac aggattcctg caaattttgt agttgtaaaa acgcttttag
     1501 cattattgcg aactacttga tcgaaatccg attccgtcat ttggtaaatc attcgatctt
     1561 ttaatgaacc tgctgaattg accaaaatat cgatatggct ataagtgtct atgcaggatt
     1621 gaatcaggtt ctcaccccct tccatggtcg aaatatcggc ataattcgct gtggcattac
     1681 ctcccgcgga tttgatttct tctacaactg aatccgccgg catgttagag gcaccggtcc
     1741 cgtctacctc agcacctttg tcgtttatta gtatggatgc accctcctct gcgaggagtt
     1801 tggcaatacc tctaccaata cctctccccc caccagtaac cgccgccacc tttccttcta
     1861 atctgtttcc catattatga ccatcctatt taattatttt ttaggtttgg caaattatct
     1921 attatcgaca attttcactt tatatcagaa ttagctaatt ataaaaggat gtctgcccaa
     1981 aataaacata gccgtcaaat ttaaagtttg aaggctattg ctccttggga aattttttta
     2041 aagacagaaa gtaaagtccg gtggctatgg cttggaatgg ggaaacaagg gttgttacac
     2101 acacatcaat taaaagtatt atgaaatctg aattaagatt cggtgaaagt agatttaata
     2161 agtatgtagg tattgtcatg agaataacaa ttactaaacc tgtaccgaaa gttacccacc
     2221 atcttttctc cacaagatcc caactcatct gaatagatct tgtgattccg gtgttgtcta
     2281 atgccatcga tgggagcgaa aaataccagc gaacaaagag gtacagcagg ataggaatac
     2341 ctattaacat tattgaaagt aggaacggta ttatcaaaag ccctatcaaa aacaatgaga
     2401 gcaggattaa cctgcctatt tttgaaagtg tgtagttgag acaggcaatg gaggagacct
     2461 tttcagagtt tgcaaattgt gctgctccaa cgataatggc cattaccgat attaggttgg
     2521 ccactagtcc tatgagaatt aaaatgaaga tagaaaacaa tttccatata ggtattgatt
     2581 tcaaatcaga cattaaattt tcggaaagct gaccatcgtt atagggctga aaattcgcaa
     2641 gggaaaccca atccgtttcg agagcagtac gtaaaataat tccgattacc agtactggga
     2701 taccatttat taaggatagg atgagaaaat ttttgaaatt ttttctatag gcgtagctgg
     2761 agattgaaat tagatctact aattttcctg gctctgggat taatcgggcc acagaaggtg
     2821 atggtatatc tgttttactt ttttcatttt ctgattcgtc tacggcacct gggttgtata
     2881 tttcccctag atcatgtgat aaaacctgac cacagttagt gcaaaatgtt tgatttctgt
     2941 tgattgtccg acaacgttca cattgggttg ctatctcttt tccgcaaccc ccacaaaaaa
     3001 gactgctttc ggtattttcg aaatcgcaat ttttgcagcc ccccattttt tatatgttgt
     3061 ttccgtacat atcaataacg aagagagggt accctaactc ctctaaattc ttttggattt
     3121 tttctttgtc accaacgaca atcattttta tttcagctgg taaaatgtgc ttcacagctg
     3181 actgtctgac atcatcgagt gtcaaatccg aaatcttttc aatgttggtc tcaaaataat
     3241 ccagaggtag gtcaaatgca gctatgttga gcaattgatt tgtaatttgt tgattggatt
     3301 caaattgaga aggcacactt tttagcaaac cttcttttgc ttttctaaat tcctcaagac
     3361 caattgggtt taattccaca atatcagtta attccttcag tgtttcaaaa actgattctt
     3421 ttgtaacctc tgtttgtaca cttcctctag tcactagaat ggagagaggt ttcatccatt
     3481 ctatggaaga atggaaccca taactatacc ctttgtcttg tcttaaattc atatttaggc
     3541 gactcgaata atcgccaccg agtatgtaat ttacaaaggc aagggaatga taatcttggt
     3601 gttctcttgg tattgatatg tgacctgctc gtaatatcga ttgggccgaa ccaggtctat
     3661 cgactaggta tatcgtagga ccttcaccgg caagaggctt tgaggcaact tgattttctt
     3721 gaggcttttg tcgattttcg aaagttctaa atttttcggt tattaattcg gctgcaagat
     3781 ttgatgaaat atttcccacc acaatgaaag tctttttaga ggtgagtaag gtttcgtggt
     3841 aatgcttttc aagatcacct gttttcattg cagcaatact ggtttcatct ccgttgatag
     3901 tatggccgta gggtgtggtg ggccccaata aaatagatct catagcaaca gatgcagtgg
     3961 catcagcact atcctggatg gaacggagat cagataattt ttcttttcgt attcgctcca
     4021 tttcagttac gggaaaattt gggttcaata ttatgtctga caagacgtca agttctttat
     4081 ttgtgttgtc ggatatgcca gaaacagcta gaaaggtgtg ctctctcgac acatctttgg
     4141 tcacgtgaga gccaaggaat tccatttcat ctgcaatttg agaactactt ctgctctgtg
     4201 tgccttcaaa caacatatcc gttgcaaact gggctattcc aggcctctct tctaggtctt
     4261 ccgctgaccc agtgtcaaag aatactccaa ctgtggtcag tggcaattca gaacgctcaa
     4321 ttactagcag ccttgttccg ttttccagaa atatgtcctt cggttgagga gctgaaaatg
     4381 tagtgagtgc tgccactttt ggtagattag ttctatcgag ttgtcttgaa gagggttttg
     4441 tgtctttaag tgggttcaca gtcaggccta cgaaattgcc gttgagcaac tttgcagctg
     4501 ctgaaacatc attagctgac acttttttgt atctttctac gtctgtctct ataaggccgg
     4561 gatttccagc catcacattg aagtggttta attggtcggc tttaccccca aacccaccta
     4621 ttttttctaa ctgtcggaca tgataggaat ttattatgtt ataggccctt tccatttctt
     4681 tgtcggatgg gggcgtttgt gatagctcat ctaataatgc ttggatctct ttatagattt
     4741 ctcctgtggt ttgtccagga tttgccgtgg ctataatatg aaattcacca gatatctctt
     4801 gtccatgatg ataagcgcga atgtccctag caagttgttt tttatagaca agattttgtt
     4861 ccagtcttga acttcttcca tcggccataa ttacagataa tacgtctaaa gccgcttgct
     4921 cttgagtaaa ttcggggacc gttggccaag ctagataaag tcgaggaaga tggacagagt
     4981 cttccaaggt gacccaagtt tgtcctttca agatggaatc cattttgtga aacctgttga
     5041 ttgcaggtcc cggcgataaa tttccgaaat atttgtctat gagatcaaag gttgaattcg
     5101 ggtctatatc tcccgcgatt gaaatgcttg cattggaagg atgatagaaa ttacgaaaaa
     5161 attcttttac gtcttctatt tctgctccat ccaaatcttc ctgagatcct atagttggcc
     5221 agttataagg atgtggaggt ggaaagagaa gttcttgtag ttttaaatac gccattccat
     5281 aagggcgatt ttcataactt tgtcttcttt cgtttttcac cacttctctt tgcaaatcaa
     5341 atcgtttctg gtcaagggcc tctagtaaaa atcccatccg atctgattcc agccataatg
     5401 ccagttccaa gtagtttgaa ggcaaattct cccaataatt tgtcctatcg tttgttgtag
     5461 acccgtttac cgtggcccct atctcctgaa gagggccgaa ataatcctta ttatgatttc
     5521 tggatccttc aaacatcaca tgttcaaata aatgcgcaaa tcctgttttg ccgggaatct
     5581 cgttttttga tcccacgtga taccaaatat tcacggccac ggttgggact gttttatcaa
     5641 catgagtgat gatatctagg ccatttccga gagtatgctt ttgaaaaggt atcgatatca
     5701 tgttctacgg cttattaggg agtgctatta ctcctgttcc aatgacaagt ttcaccccat
     5761 cagaattttc catcactacg tcacattcta attttgtact gtccccagtc tcatctttat
     5821 ctgtcactat cccactgaac gtcaggtctt ggttgtgagg aactgtttgt cggaagacga
     5881 cgtctatttt gagaagacaa actgtgtctg accagtcggt taaaatttgt gacatcagcg
     5941 ctatattcat gggacctggc acaatggctt ccggcatccc ctcgttattt gctatttctt
     6001 ttgacgtaaa tcttgaaggg gtgatttctt tttctctgat tgaaacaaat tcgatcacat
     6061 ctttgtcagt gacggatttt tttatcggac caatatcgtc tccgatttca acatcttcaa
     6121 aatatagatt ctctttcatc tatttttatt ccttctaaca aaagactctg ttactaaggc
     6181 aacggtctct tcatcctggt tgctgaattt tgtttcccaa acagcaaaaa ccatttttcc
     6241 ggaccggcct gttttggcat aaactttgtc cagcttcgta ctggctgaaa gaacatcgcc
     6301 aggtcgaatt tctgctttac attcgataga ttgaccagcg aaaaacccca attctccaaa
     6361 ttcaagcttt atgtcgggtc ttgatacccc tgaaacaaaa acattgcaga ttgttggtgg
     6421 ggctacgata tgcccggcac catgttcatc ccccacgtac cttggattag tttcacctgt
     6481 cgatttcgca aaccctaata gcatttccct ggttaactca aaagttccaa tctgtgtttc
     6541 cactccaagc aaacttctgt cgtatacgat tccttcatct gtcgtcattc tgtgctccaa
     6601 aatatgtggt caggattcaa gtttcacagc ggtaaaacgg ccatttctta gttcctgtac
     6661 taaatcagat tcattcgcta tttttttttc aaaacgtgtc atgcaggtac ctaccgcact
     6721 cactatgtgg gcgtcgcttc ctcctgttcc taaatacccc ttcgctttaa taagtgattc
     6781 ggctttttct ccttctcctg gcctgttgga cccgttaagc tgttcgacag ttttaactgt
     6841 ttcaaaacca gttacaccat tatctaaata ttcagcaaaa cctattccaa ctcgaccggg
     6901 atgagctgga attgctattc ccccataggt gtcgcaaaca tcaactaggt tttgtgcact
     6961 aatgttaaca tctgagaaat ccaattcatt ggtgatttgt tctgagatcc cgtaaacgag
     7021 aaaatgcccg gtgtctgtgt ctaattcagc ccctttcaaa atgattattc catcatctga
     7081 ggctaactta ctatagtcca catcgtggtc gaattgcctg tgctcggtta agacaaatcc
     7141 atgtatcgat ttgccttttc ttcgtagcac attaacccat tttatgtatt gttctaccgt
     7201 tgctctggaa tcatccgagg caactgagtg agtgtgtaaa tcaaggagca taacaggatt
     7261 gaatgttagg tcagcatatt catgtagtca agaaattaat gtattaaact tagggaaatt
     7321 gcatccaata gatgtaaaac acaacaaagt gggtgattaa tatggattta gggttgactg
     7381 gaaaaattgc tttaataact ggcggcagta gaggtttggg tagagaaagt gcattgtcct
     7441 tagctagaga aggcgtgagc gttgccatat gtggtagaac aaaaaatgta ttagatgcga
     7501 ctgtaaaaga gattgagcaa attggggcaa cctgcgtggg cattgtggct gatgtatctg
     7561 ataccagggc tatagaagtt ttgcattcat ctgtaattga ggccctcgga cctatagata
     7621 tactcgtgaa taatgcaggt ggaaccagat ctagagatga tattaatgga acccctttag
     7681 aggattttaa aggcaccttt gatttaaatg tttttggagc atttgaattg atgcaacatg
     7741 ttataccgtc tatgagaagt aacggttggg gcagaattat taatattgct tctatctggg
     7801 gccgagaata tggcgggaat atatcttaca tgtcggcgaa agcggcatta atcgcagctt
     7861 ctaaacactc ggcaatttcc ctggctaaag atggaatctt ggttaattct attgctcctg
     7921 gctcaatcga acaccctgga ggttcatggg aacgttttca aaatgacaat tcgcctcaag
     7981 tcgtagaaaa ctttataaag gaaaatctgc ctcttggcag gtttggctgg cctgaaccgc
     8041 ttggagattt ggttgcctat cttgcctctg atagatctgg gctaattacc ggttcatgta
     8101 tagtaatcga tggtggtcag agcgtatcga tgatctagaa attagttttc taaagacctt
     8161 ttggggttgg gatgttctaa tacttctgga ttgactaaat tttcaggcat ggtattcctg
     8221 tacactttga cgacctcgct ggctgctcgt tcttctagct caagggttga ttcctgggag
     8281 aagaaagctg tgtgaggggt tatcagtatg ttttccagtt aaattaaggg atgatcccgt
     8341 gatgtttcgt tatccaccat cacatctaga cctgcaccac ctattttacc ctctgataag
     8401 gcctcgtata aggctatttc atcaattaag ggacctcgtg cagcattgat taggaaagca
     8461 tcgtctctca tcaaatcaaa ttgttccgat ccgatcattt tctctgtgtt gggagttaat
     8521 ggtgcatgaa gtgaaacaaa atccgaattc tccagtagtt cctcaaggct tacca
//
LOCUS       DASCNS010000302         9916 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002178667, whole genome shotgun sequence.
ACCESSION   DASCNS010000302 DASCNS010000000
VERSION     DASCNS010000302.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002178667"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(44..550)
                     /gene="purE"
                     /locus_tag="QF838_10595"
     CDS             complement(44..550)
                     /gene="purE"
                     /locus_tag="QF838_10595"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HJO61166.1"
                     /translation="MTELQNSSGPTVSVVGGSTSDFPLLRQATEILDQLAIDNELRVV
                     SAHRTPDWLYEYAETAENRGIKVIIAGAGGAAHLPGMLAAKTIIPVIGVPVPLEYLKG
                     MDSLMSIVQMPRGVPVATVAIGGAENAAILAAEIIAVSDSKVRDKLRSWRENLTETVL
                     KNPEAKGD"
     gene            complement(798..1049)
                     /locus_tag="QF838_10600"
     CDS             complement(798..1049)
                     /locus_tag="QF838_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012725.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJO61167.1"
                     /translation="MKSCSWQEQFRKGNCLANRGDHVETIKCFLVTIRHNPEHAMAWN
                     NIGVAKLCLSKPDEAVIAFEKALEIDPDYRDALHNTALA"
     gene            1163..2338
                     /locus_tag="QF838_10605"
     CDS             1163..2338
                     /locus_tag="QF838_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HJO61168.1"
                     /translation="MDSANQDLNLSTSILPGMVDASPEMHHAKLNLTAKINTILTKYG
                     YDIFDCPIMESTELYAMKSGGELTSRLYSFVEPGGISVSLRPEFTSSVIRFYVSNEAV
                     HKKTVKKQYLGPVFRYFDSNQGLNQRQFTQQGCEIIGDSSIGSDVEILKLSLLALAES
                     GLEKVTIKLGHMGYIRSILEDFELSEALIGFTLSNLQVLNESESGIAGLVDMASGIGL
                     INPGENHDETTNIKTAETPMSSLGRRTFEQITNRSLKKKSHLFSVEKYSKALTRLSLF
                     LSTFSGDINNVSNNMTTDPIHKDAAKYFKRVIKESANVSDMAVDFIVDCAAQRGFTYY
                     TGIIFDIEHVSDNKSIPLGGGGRYDGLVKLLGGPEEIPATGFAVNVDSILSIITVAE"
     gene            2341..3090
                     /gene="hisG"
                     /locus_tag="QF838_10610"
     CDS             2341..3090
                     /gene="hisG"
                     /locus_tag="QF838_10610"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HJO61169.1"
                     /translation="MLSEATELKLIIPSDGAMYESTLEFMEKVGLSVTRESKRRYIAH
                     IPNLSGVTVLLLRSADITSTIEDGSADVGLLGEDRYLELRQENLPTEILLANLKYGNC
                     SLTLGVPNSWADVSSIADVAEVAVEFRERGLSMRVATKSPRLTERYLLSHGINYFSLV
                     RLSGTLELAPEMGFADIIADITSTGTTMKENQLKTIQGGTIIESEACLIGNTNELSSN
                     VIKLGTAKQFIKTIISNFESKSSGIDVRYNL"
     gene            3119..3865
                     /locus_tag="QF838_10615"
     CDS             3119..3865
                     /locus_tag="QF838_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013078.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HJO61170.1"
                     /translation="MPNLPTHIHFSFESLGKYNDLDLSKNLDVYLLGSTAPDIRVITK
                     QDRSFYHFVKLDFDGVGDGIRNMQSFHPQLNYLKEKDSHTRSFMAGYASHLILDETWI
                     TTMFRPFFSDVNLFGGRNQGLILDRALQMQLDKSYWKTLEQNLAHVESCEIEVDVEFL
                     REEPLKEWQKWISTLLNREFSWDRLEFMANRIAKGNSQHPVVDLAKDFIADPVGGVDD
                     ILERLPNGILEEFSNQSLENIDKALKEFTL"
     gene            3862..5184
                     /gene="hisD"
                     /locus_tag="QF838_10620"
     CDS             3862..5184
                     /gene="hisD"
                     /locus_tag="QF838_10620"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HJO61171.1"
                     /translation="MRKVIGVEQSLDILSKNRGIDLEGDPKYISQFSEAIFGEKMSLL
                     DYVKEILTKVKTGGDKAIYDLSSKIDGQNLKQISMGKKDLENAYKHIPPNLREALEFS
                     ANRIKNFHGRSMPTDWHDNAEGYGQRYVPVNSAAAYIPGGTAKYPSTVLMTAIPARVA
                     GVEEIAITCPMPKSPLEYASVLAAAYISEVDQVYCVGGAQSIGALAYGTESVSKVDFI
                     CGPGNVFVTLAKKLVYGEVGIDGLYGPTETVVIADEHANPTLCAADLIAQAEHDSLAR
                     PILITTSPNIAADVEAEIYLRLSRLDRSEIATKSIETQGFIAIVNNLEEALELSNSFA
                     PEHVCIATNDPEKLVPAIKNAGMIFVGELSHEVLGDYGAGPSHVMPTAGTARFNSGLG
                     VQSFLKTIPVVTIDSDNSSKLGHVASVIAREEGLTGHAEAAEIRIELN"
     gene            5231..6319
                     /gene="hisC"
                     /locus_tag="QF838_10625"
     CDS             5231..6319
                     /gene="hisC"
                     /locus_tag="QF838_10625"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HJO61172.1"
                     /translation="MEIVDIQKIIRPHLVDIKTYEAMEAPEALAKKSGIPESEIIKLN
                     GNENPYGGSQKALDAVAKTPLHIYPDPNQTNMRIALSEYTGMPSENIVVGSGADELID
                     LIFRLFISEGDKILDCEPTFGMYSFCARVAGGEITLVQRDEQFDIDVPSVLESIDAKT
                     KVIFVTSPNNPTGNIATESQVTDLLGTGLLVVVDEAYFEFSGQTVQHLVSQNENLIVL
                     RTMSKWAGLAGLRVGYGIMHRKLVKYFMDVKPPYNVNIAAESALIASLEDVNYLLSNV
                     GKIIEERERMFELINNLPRAKAWPSHGNYLMCEFEKNEAERVYVELAAKGIFVRNFNS
                     PRLRDCFRIAIGTASDTDKLMAAMKIII"
     gene            6333..6929
                     /gene="hisB"
                     /locus_tag="QF838_10630"
     CDS             6333..6929
                     /gene="hisB"
                     /locus_tag="QF838_10630"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HJO61173.1"
                     /translation="MSRTSENKRKTTETDISVKVNLDGTGNYKVESGNGMFDHLLAQI
                     SRHGLIDLEIYASGDTHVGWHHLVEDTAIVLGRSFKEAVGEGKGIVRMGHTLVPLDEA
                     LVLTVIDYSGRGYSIIDSPLTESDLGDLPSDLIRHFMETFSREGGFNLHLTVMAGINN
                     HHIAEASFKSIARSLKSALSFDPRQGEAISSTKGTISG"
     gene            complement(6930..7181)
                     /gene="purS"
                     /locus_tag="QF838_10635"
     CDS             complement(6930..7181)
                     /gene="purS"
                     /locus_tag="QF838_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HJO61174.1"
                     /translation="MAFAANIYVSLKSTVSDPQGATVSGALSQLGFDSVKDVRVGKYI
                     TLHIDKSSKKEAESVVADMCEQLLANTVIEEYKFELQEL"
     gene            complement(7185..7832)
                     /locus_tag="QF838_10640"
     CDS             complement(7185..7832)
                     /locus_tag="QF838_10640"
                     /inference="COORDINATES: protein motif:HMM:NF022747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HJO61175.1"
                     /translation="MSTQNPKNRPTPTQARAARRGRRQNRRKFTRVFIGLAIGGVALL
                     LILGLILPMLGDLGSSADKVPDGPGKRIESDGKDHIDEGSEHPAYRTVPAASGWHYPQ
                     PLAPVSWGIHTEYIPEEKRIHNLEHGGISIAYNCAEPTSDDCKQLVESLGSIVGRARE
                     ANLKVVMSPYPGMPTKIALTAWTFIDAFDEFDEDRIKDFINSHESSPNAPERFSN"
     gene            complement(7864..8754)
                     /locus_tag="QF838_10645"
     CDS             complement(7864..8754)
                     /locus_tag="QF838_10645"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJO61176.1"
                     /translation="MSIDSSDILLESNVAKLVHRGKVRDTHSIDEETLLMVSTDRVSA
                     YDVVMPNAIPGKGAILNQMSVFWFGMTNHIVPNHLINFAMDMEDMGLSEAVRRRSMIV
                     KKAERIDIECVARGYITGSALQEYEDNGMIAGVSMPTGMKEGDLFERPIFTPATKAET
                     GHDENISIEKMEDMIGGELTHLLQQMTLEVYRFAHGYALNKDIIIADTKMEFGFIDGE
                     LSLIDELLTPDSSRFWDKNLYSPGSTQPNYDKQFVRDWLANQGWDKEPPAPKLPDDIV
                     NKTSIRYREAFDKLTEIDLR"
     gene            complement(8757..>9916)
                     /gene="purB"
                     /locus_tag="QF838_10650"
     CDS             complement(8757..>9916)
                     /gene="purB"
                     /locus_tag="QF838_10650"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HJO61177.1"
                     /translation="VKIRKANYDFDLFYEILDRTKHDMTAFLQSTTANLGPEGRWLHQ
                     GLTTSDVWDTATSLQLVEAAQLLDFELEQLEAALKQTAIKHKNTLMMGRTHGVHAEPI
                     TFGLKIALWWDEVKRGRKRLHEATGSISVGKLSGPVGTHATAPPSIEKDVCDLLGLSP
                     APISNQVIQRDRHAHFITTLALVAATLEKIATEIRGLQRTEIREVEEPFTEGQTGSSS
                     MPHKRNPELTERVCGLARLIRGHSITAMENVALWGERDISHSSAERLILPDSCLALDY
                     ITNLLTGVIKGLRIYEDRMLENMELTKGLLFSQRTMLLLIEKGLAREDSYKIVQENSM
                     KTWDNGLDFRELISNDHRVTDIVSPQELADLFDYSHYTKYVDEIFDKAFLS"
BASE COUNT         2962 a         2079 c         2061 g         2814 t
ORIGIN      
        1 ctaatgttga gccaggagga agtatcgaaa ctgatgacat taactaatca cccttggctt
       61 cagggttttt tagcacggtt tcagtcagat tttcccgcca ggatcttaat ttatctctta
      121 ctttagaatc ggaaaccgct attatctctg ccgccaaaat agcggcattt tctgcacccc
      181 ctatagccac cgttgcaaca ggaacacctc ggggcatttg cacaatagac atcaaagaat
      241 ccattccttt caaatattcc aatggcactg gtactcctat aaccggaatt atagtttttg
      301 ctgcaagcat tcctggtaaa tgagccgccc ctccggcacc cgcgatgata actttaatcc
      361 cgcgattttc ggctgtttcg gcatattcat aaagccagtc aggggttcta tgagcggaaa
      421 ctactcgcag ctcattatca atggctaact gatctaaaat ttccgttgct tgcctcaaaa
      481 gtgggaaatc tgatgtactt cctccaacaa cagaaactgt tgggccagaa ctattttgta
      541 actctgtcat ttccgcctcc attttcgata tattttatat ctatttatag tttcatgatc
      601 cgatttaacc ttttaataga accctagtgt ccataagtca attctttgaa tttaatataa
      661 tctttgaaac tttgttcaga atttccaagc atctggttaa gtatgctgcg atgtttgtat
      721 gacgcccaat gtccaggatc tagtgagatc gctcggttca aatcatccat tgctgaatct
      781 aattcaccga catctgacta ggccagggcc gtgttatgca acgcgtctcg gtagtctgga
      841 tctatttcca aagccttctc gaaagcaatg acagcctcgt ctggcttaga caaacaaagt
      901 ttcgccactc ctatgttgtt ccaggccata gcatgctcag gattgtgtct gatagttact
      961 agaaaacact ttatagtctc aacatgatct cctcggttgg cgaggcagtt acctttccta
     1021 aattgctcct gccaactaca agatttcaat attaattcct cttttgggga ctgaaaaatc
     1081 acttacctgg tgagacagct gcatttgaag ccatgatttc accatactaa aattaacatg
     1141 cctattgtag tatttatgga caatggattc agcaaaccaa gatctaaatc ttagtacatc
     1201 aatcctccct ggcatggtag atgcatcgcc agagatgcat catgccaaat taaacttaac
     1261 tgcgaagatt aatacgattc tcacaaaata tggctacgat atatttgatt gcccaataat
     1321 ggaatcaact gaactgtatg caatgaagtc cggaggtgag cttactagtc ggttatattc
     1381 cttcgtagaa ccaggcggta taagtgtcag cctgagacct gaatttacat cttcagtgat
     1441 aagattctat gtctcaaacg aggccgttca caaaaaaacc gtcaaaaagc aatatctcgg
     1501 tccggttttc aggtattttg attccaacca agggttgaat cagagacaat ttacccagca
     1561 aggctgcgaa ataattggtg attccagtat agggtccgat gtagaaattc ttaagcttag
     1621 cctgctcgct ttggctgaat ctggtttaga gaaagttaca atcaaactag gtcatatggg
     1681 ttacataaga tcaatacttg aagatttcga actaagtgaa gccttgatag gattcacact
     1741 ctcaaatttg caagttttaa acgaaagtga gagtgggata gctggtttgg tcgatatggc
     1801 ttcagggata gggctaataa atcccggaga aaaccatgat gaaacaacga atatcaagac
     1861 tgccgaaact ccgatgagta gtctgggtag acgaacgttt gaacaaataa ctaaccgatc
     1921 cttgaaaaaa aaatcacatt tgttctcagt tgaaaaatat tcaaaagcac tgacaagact
     1981 ttccttattt ttgagtactt tctcagggga tatcaacaac gtttccaata acatgaccac
     2041 tgatcctatt cacaaagatg ctgcaaaata ttttaagcgt gtgataaaag agtcagctaa
     2101 cgtgtctgac atggcagtcg atttcattgt cgattgtgct gcacaacgtg gatttacata
     2161 ctacacaggc atcatttttg atatcgaaca tgtgtcagac aacaaatcaa ttccgctggg
     2221 aggaggtggg aggtacgatg gcctggttaa acttttaggg ggtccggaag aaataccagc
     2281 tacaggtttt gcagtaaacg tggattccat actttcaata atcacggtgg ctgaataaaa
     2341 atgctgtcag aagccactga actgaaatta attattccga gtgatggggc aatgtatgaa
     2401 tcaactttgg aatttatgga aaaggttggg ctatccgtca caagggaaag caaaagaaga
     2461 tatatagctc atatcccaaa tttatcggga gttactgtcc tgctacttag aagcgccgac
     2521 ataacctcca cgatagaaga cggtagtgct gatgtaggct tactgggtga agatcgctat
     2581 ctagaactta gacaggagaa tctaccaacg gagatcctat tagcaaacct aaaatacggt
     2641 aattgttccc ttacccttgg agttcctaat tcatgggcag atgtgagttc tattgctgac
     2701 gtggccgaag tggcagtgga attccgagaa cgagggttga gtatgagggt tgcaaccaaa
     2761 tcaccacgcc ttacggaaag gtatctactt tctcatggaa taaattattt ttcgctagtc
     2821 cgattaagtg gaacgttgga gctcgcgcct gaaatgggct ttgctgacat aatagctgat
     2881 ataacttcaa ctggcacgac catgaaagag aaccaactga aaacgataca gggtggaaca
     2941 attattgaat cagaggcctg tctgataggt aacacaaacg aattgtcctc aaatgtaatt
     3001 aaactaggta cagcaaaaca gtttataaag acaattataa gtaattttga gagtaaatcg
     3061 agtgggattg atgtcaggta caatctgtag aaagaaaagt taaaagccgt ctataaatat
     3121 gcctaacttg cccactcata tacatttttc gtttgaatca cttggaaaat ataacgatct
     3181 cgacttgagc aagaatctgg atgtatatct tttagggtcc acagctccag atataagagt
     3241 gataactaaa caagatcggt ccttttatca ctttgtaaag ttggattttg atggggtggg
     3301 ggatgggatc cgaaatatgc aatcctttca tcctcaactg aattatctca aggaaaaaga
     3361 tagccataca aggtcattta tggctggata tgcctctcac ctgattttag acgaaacatg
     3421 gattacaaca atgtttcgtc catttttttc agatgtgaat cttttcgggg gcagaaatca
     3481 aggtcttatt ctggaccggg cgctccaaat gcaattagac aaatcgtatt ggaaaacttt
     3541 ggaacaaaat ttggcacatg ttgaatcatg tgagatcgag gtagatgtgg aattcctgcg
     3601 ggaagaaccg ctgaaagaat ggcagaaatg gatatccaca ttgctcaacc gagaattctc
     3661 atgggacaga ctggaattca tggctaaccg aatcgccaaa ggaaattcac agcacccagt
     3721 ggtagacctt gcaaaagatt tcatcgcgga cccggtgggg ggtgtagatg atattctcga
     3781 aaggctccca aatggaattt tagaagaatt ttcaaatcag agtttggaaa atatagacaa
     3841 agctctcaaa gagtttacct tatgagaaaa gtgatcggag tcgaacaatc tctagatatt
     3901 ctttctaaaa ataggggtat cgatctagaa ggggatccca aatacatctc acaattttct
     3961 gaggctatat ttggggagaa aatgagttta ttggattatg taaaagaaat acttactaag
     4021 gttaaaacag gtggagataa agctatttat gatctttcat caaaaataga cgggcaaaac
     4081 ctgaaacaga tttctatggg taaaaaagac cttgaaaatg cctacaaaca catacctccc
     4141 aatcttcgcg aagctctaga attttcggcc aatcgtatta aaaattttca tggacgatct
     4201 atgccaaccg attggcatga taatgctgaa gggtatggtc aaagatatgt tcctgtaaat
     4261 tcggcggctg cctatatacc aggtggtacc gcaaaatatc cttctacagt gctcatgaca
     4321 gccataccag ctagggtagc cggagttgaa gaaatagcta taacatgccc gatgcctaag
     4381 agtcctttgg aatatgcttc cgtgcttgca gcagcgtata tatcagaagt agatcaggtt
     4441 tattgcgtag gaggagcaca atccatcgga gccttggcat acggtaccga atcagtatcg
     4501 aaagtagatt ttatatgcgg cccaggtaat gtgtttgtca ctttagctaa aaaactagtc
     4561 tatggagagg ttggcataga cggcctctat gggcctacag agactgtagt gatcgccgac
     4621 gaacatgcta acccaacatt gtgtgctgct gacttaatcg cccaagctga gcatgattct
     4681 ttagcacgtc caatcctcat aaccacctct ccaaacattg ctgcggatgt agaggctgag
     4741 atttatttac gcttatccag attagaccga tcggaaatag ctaccaaatc aattgaaact
     4801 cagggattta tagcaatagt caataatctt gaagaggctt tggaattatc aaattcattc
     4861 gccccggaac acgtctgcat agctaccaat gacccagaga agttagtccc ggcaataaaa
     4921 aatgccggta tgatttttgt tggagaatta tcacatgaag tactcggaga ttatggagcc
     4981 ggtccaagcc atgtaatgcc taccgcaggc acggcccgtt ttaactctgg gttaggggtg
     5041 caaagttttc ttaagactat acctgtggtt accatcgatt ctgataattc ttcaaaatta
     5101 gggcatgtgg cgtcggtgat agccagagaa gaaggtctaa ctgggcacgc agaagcagcg
     5161 gagataagaa tcgaacttaa ctagtcctat aatatggttg attacaactt ttcaaagaaa
     5221 ggctatacat atggaaatag ttgatataca aaaaattatt cggccacatc ttgtggatat
     5281 taaaacttat gaagcaatgg aggcgccaga ggccttagcg aagaagagtg gtatcccaga
     5341 gtcggaaatc atcaagttga atggaaatga aaacccatat ggcggttctc aaaaagcttt
     5401 ggatgccgtc gctaaaaccc cgcttcatat ctacccagac ccaaaccaaa caaatatgag
     5461 aattgcctta tcagaataca ctggtatgcc atctgaaaat attgttgtgg gatcaggggc
     5521 tgatgaacta atagatctaa ttttcaggct gtttatatct gaaggagaca aaatattaga
     5581 ctgcgaacct acctttggaa tgtatagttt ttgtgcaaga gtggcaggag gtgaaatcac
     5641 actggtgcaa agggatgaac aattcgatat tgatgtgcct tcagttttgg agagcataga
     5701 tgccaaaaca aaggtcattt ttgtaacttc cccaaacaac ccgacaggta atatagccac
     5761 tgagtcccaa gtgactgatc tactcggtac gggactttta gtcgtagtcg acgaagccta
     5821 tttcgaattt tcaggccaga ctgtacaaca tttagtttcg cagaatgaaa acctaattgt
     5881 gctcagaact atgagtaaat gggcaggatt agctggactg agggtgggtt acggtatcat
     5941 gcaccgtaaa ttggtgaaat atttcatgga tgtaaaaccc ccatacaacg tcaacattgc
     6001 cgctgaatct gcacttatag cttcattgga agacgtgaat tatctcctaa gcaatgtggg
     6061 gaaaataata gaagagcgag aaaggatgtt tgagctaata aataacctac cacgtgcaaa
     6121 agcttggcct tctcatggta attatttaat gtgcgaattc gagaaaaacg aggcagaaag
     6181 agtatatgtg gaattagccg caaagggtat attcgttagg aactttaact cccctagact
     6241 gagggattgc ttcagaatcg ctatcggtac tgcctcagac actgacaaat taatggcggc
     6301 catgaagata ataatttaac gggataataa aaatgagtag aacctctgaa aacaaaagga
     6361 aaactaccga aaccgacata tcagtaaagg taaacttgga tggtaccggt aattacaaag
     6421 ttgaatccgg taatggaatg ttcgaccatt tgttagctca aatttctcgg catggattaa
     6481 ttgatcttga aatttatgcc tccggtgaca ctcatgtcgg ctggcatcac cttgtagaag
     6541 atacagccat tgtgttggga aggtccttca aggaggctgt cggggagggt aaggggatag
     6601 tgaggatggg ccacacattg gtaccgttgg atgaagcatt agtcttaacc gtcatagact
     6661 atagtgggag agggtattca atcatcgatt cgcctctcac agaaagtgat ttgggagatt
     6721 tgccttcaga cttgattaga cattttatgg agactttttc acgggaaggt ggattcaacc
     6781 tacaccttac agtcatggcc ggaataaaca accatcatat tgcagaggcc tcattcaaaa
     6841 gcatcgccag atctctaaaa tccgctcttt cgtttgaccc acggcaaggg gaagccatat
     6901 ctagcacaaa aggtacaatt agtggttgat tacagctcct gaagctcgaa tttatattcc
     6961 tctatgaccg tgtttgcaag taactgctca cacatatcag caacaactga ctcggcctct
     7021 ttttttgaag atttgtcaat gtgtaatgtt atatattttc caacccgaac atctttaaca
     7081 gaatcaaacc ccagctggga cagggctcct gaaacagtcg ccccttgcgg gtcactgacc
     7141 gtagatttca aactaacgta tatatttgcg gcaaaggcca tggtctagtt ggaaaacctc
     7201 tctggtgcat tcggtgaact ttcatgagaa tttatgaaat cttttattcg gtcttcatca
     7261 aactcatcaa aagcatcaat gaaagtccat gccgtcaaag ctattttagt aggcatcccc
     7321 ggatacggcg acattactac tttcaaatta gcctctctgg ccctaccaac tatgctcccc
     7381 agtgactcaa ctagttgctt acaatcatcg ctagtaggtt cagcgcagtt atatgctatg
     7441 gaaatacccc catgctcaag gttatgaatt cgtttctctt ctggaatata ttctgtatgt
     7501 attccccatg aaacgggagc aagcggttgc ggatagtgcc aaccagatgc agctggtaca
     7561 gttctgtatg ctggatgttc tgaaccttcg tcaatatgat ccttcccgtc ggattcaatc
     7621 cgctttcctg gtccatcagg cactttatca gctgaactac ctaaatcacc aagcataggc
     7681 aaaatgagcc ctaatataag taaaagagca acgcctccga tcgccaaacc tataaagact
     7741 ctagtaaatt ttctacggtt ttgtcttctc cctctacggg cagcgcgagc ttgggtgggc
     7801 gtaggtctat tcttaggatt ttgtgtactc atattctttt atcaaaacac catatttaat
     7861 tttttatcgt aggtctatct ctgtaagttt atcgaaagct tcccgatatc gtatactcgt
     7921 tttgttaaca atatcatcag gtaatttagg tgcagggggt tctttatccc atccttggtt
     7981 agctaaccaa tctcgtacaa attgcttatc ataattcggt tgtgtactac ctggagaata
     8041 taagttttta tcccagaacc tactggaatc cggggttaac aattcatcaa ttaaactcaa
     8101 ttcgccgtca atgaacccaa attccatctt cgtatcagca ataattatat ccttattcag
     8161 ggcatatccg tgggcaaatc ggtatacttc caacgtcatc tgttgcagca agtgggtcaa
     8221 ttctccccca atcatatcct ccatcttctc tatggatata ttctcgtcat ggccagtttc
     8281 agctttggtg gcaggggtaa agattggacg ttcaaaaagg tccccttcct tcatacctgt
     8341 aggcatggaa actccggcta tcatcccgtt atcttcgtat tcctgaagag cagatcctgt
     8401 tatgtatcct cgagccacac attcaatgtc gatcctctct gcctttttta ctatcatgga
     8461 tctacggcgt acggcttccg aaagacccat atcctccata tccattgcaa aattgatcaa
     8521 atgattagga acgatatggt ttgtcattcc aaaccagaac accgacatct gatttagtat
     8581 tgcccctttc cctggtatcg cattaggcat cacaacgtcg taagcagata ccctgtcagt
     8641 ggataccata agtaaagttt cttcatcgat tgaatgcgtg tcgcgtacct tgcctcggtg
     8701 taccaattta gcaacatttg attctaataa aatgtccgaa ctatcgattg acatagctaa
     8761 ctaagaaagg ccttatcgaa aatttcatca acgtactttg tgtaatgaga ataatcgaaa
     8821 agatcagcta attcttgagg ggacactatg tcagtcaccc tatgatcatt tgatattaat
     8881 tctctaaaat ccaacccatt gtcccaagtt ttcatggaat tttcctgaac aatcttgtag
     8941 gaatcctctc ttgcaagtcc tttttctatt agcaaaagca tggtccgctg agaaaataac
     9001 aaacctttag tgagttccat attttccaac atacggtcct cataaattcg gagtcccttt
     9061 attacccccg tcagtaaatt cgtaatgtaa tctaatgcca ggcaagaatc gggtagtatc
     9121 aatctttcag cagatgagtg gctaatatcc ctttcacccc ataacgccac attttccata
     9181 gcggttattg aatgacccct aataagccta gccaaaccac atactctctc agtcaattcc
     9241 ggatttcttt tatgtggcat tgaggaggac cctgtttgac cttctgtaaa tggttcctct
     9301 acctccctaa tttctgtcct ttgtaagccc ctaatttcag tggcgatttt ttcaagtgtt
     9361 gcggcaacca aggccaaggt tgtaataaaa tgtgcatgcc gatccctttg gataacctga
     9421 ttagagatcg gtgcagggga caagccgaga agatcgcaaa catccttttc aatcgatggt
     9481 ggagccgtag catgcgtgcc aacaggacct gaaagctttc cgacagatat gctgccggta
     9541 gcttcatgaa gtcttttcct accacgcttc acttcatccc accaaagagc aattttcagc
     9601 ccgaaagtaa taggctctgc atgaaccccg tgggtccgac ccatcatcag agtattttta
     9661 tgtttaatcg ccgtttgttt cagtgcggct tctagttgtt ctaattcaaa atctaaaagt
     9721 tgcgcagctt cgaccaattg taggcttgtc gctgtatccc aaacatcact tgtagtaagc
     9781 ccctggtgca accagcgccc ttctggtccc aagttggccg tcgtagactg caaaaaagca
     9841 gtcatatcgt gctttgtacg atccaatatt tcataaaata gatcaaagtc ataattagcc
     9901 tttctgattt tgacta
//
LOCUS       DASCNS010000303         8817 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002180895, whole genome shotgun sequence.
ACCESSION   DASCNS010000303 DASCNS010000000
VERSION     DASCNS010000303.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002180895"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="QF838_10655"
     CDS             complement(<1..599)
                     /locus_tag="QF838_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015418.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HJO61178.1"
                     /translation="MATHHHGHHHEDMLSVEEARDKILSQFGVLNAQNFLITEALGLV
                     TSDQVRSEINIPPLDNSAMDGYAVMSRDVSTATYEHPVMLEILETIPAGSLPTKPLKT
                     GAASRIMTGAPVPEHCDAVIPFEDTTEKDFSKSGRLTEIGIRTSVSPGTNVRVKGKDI
                     KEADSVLEEGHVITAATIGVLASMGMSHVRAIRKPVVSIL"
     gene            complement(626..1123)
                     /locus_tag="QF838_10660"
     CDS             complement(626..1123)
                     /locus_tag="QF838_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61179.1"
                     /translation="MSFTKSAGITYETGKVADIFLNQILIGHIGELKSNLSEFYDLSS
                     SPVIIFRLFLDSIEKVAEVQFVDYSEPSRYPSSFRDLALIADKEIDSQSILSTISKNK
                     LVANSFPIDQYEGGEVPDGKHSITMRIIFQSDDKTLSAKEIDRAHVQIIKSLEHQYKI
                     SERYS"
     gene            complement(1060..3060)
                     /gene="pheT"
                     /locus_tag="QF838_10665"
     CDS             complement(1060..3060)
                     /gene="pheT"
                     /locus_tag="QF838_10665"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HJO61180.1"
                     /translation="MKIPLSWLRNYVDIDSDVTDLAHSLTMAGLEVGSIEQIGDKWDS
                     DSLVVAQVIELEAHPNADRLRLPTLDLGKGEKAKVVCGAPNLEIGQKIVFAKEGANLF
                     NPRSGKHEILKGANIRGIESKGMVCSALELGIGEDSGGILVLDNESITGTSVKDLLSD
                     SILDTELTPNRPDWLSIIGTAYEIAALLARDLIQPESSYQSGDTKIEDCIKVTIQDSL
                     NCPRYTGSFIENVKVAPSPVWLQDVLTKSGHRPINNIVDITNYVMLEYGQPLHAFDFN
                     KLEGNEIIVRQAFGSERLETLDSQPRTLTPPMLVIADTSKPIGLAGIMGGVNTEIDEY
                     TTDIFLEAANFDAANTRATRSALGLNTEASYRFERDIRHELAPIALKRATYLISQLCG
                     GIPAEGIIDTFSGSHHIKTIEITEARFEKILGTPVFMKDIWNIIKRLGFQKIEEDVTH
                     VINVIPPIWRSDISIQDDIIEEYARIYGYDNLPTSNLSSSIPSLGSKNPQRVREHMRD
                     RLVSAGMNETISYSVTDLETINLFDSISNDQEPITIANPMDASKRYLRPTLVPNVLET
                     LSRNRRTDQQAGLRLFEIGRVFLPLETDEADSMPVEKEEIVGVITGLSNSTDLWSESD
                     RVMDFFDAKGVVEHIFHGLSGFCHLLKVLESLMKQAKWPIFF"
     gene            complement(3072..4133)
                     /gene="pheS"
                     /locus_tag="QF838_10670"
     CDS             complement(3072..4133)
                     /gene="pheS"
                     /locus_tag="QF838_10670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HJO61181.1"
                     /translation="MNEKSYLEIRIQIEKDALFSLSQIETLNELETWRINTLGRNGKV
                     TGLLRGISDLPVDSRRKAGGEANSLKNLLTEKYESQKSGIENKENNVTGNGPELLDVT
                     LPGRSISLGSLHPTTAAVREICQVFESMGFQTIEGPEVELDKYNFQKLNIPEEHPARD
                     MWNSLWIDRELPDGSRPLLLRTHTSPMQIRTMESQSPPVRVVVPGKTYRYEATDATHE
                     WQFCQIEGLAVDKDITFANLKATLEEFARRIFGDKRKARFRCDFFPFVEPGAEVSIDC
                     FKCEGDGCRVCGNTGWIEIMGAGMVHPKVLAGVGYDPEIYSGFAWGMGAERIAMLKHG
                     IDDIRHFYSNDMKFLKQFS"
     gene            4374..5501
                     /locus_tag="QF838_10675"
     CDS             4374..5501
                     /locus_tag="QF838_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HJO61182.1"
                     /translation="MEISQLTDSALVGAIAAGLILVAFVILAIFLRVTLAILTKRADK
                     QKNDRLLLVFIGSIKGPSVLLVISLGLLFAYFIIARIDEGIFTSIAGTGEYAFRVWQV
                     SAIFMATFTFSHLSDRMIKWYLANVAGKTATTLDDTLLPIFRRVLPITVYAVGTLIAI
                     DNLGVSISPILAGLGIGGLAVALAVQPTLSNFFAGTYVVTEGELKAGDFIELDGGPSG
                     YVESVGWRSTKIRSRFNNLVIIPNSRMAESIVTNYFSPTPAMNVIVTCGVSYESDLEQ
                     VENIALEEAEALVKESRHSIKDMDPFFGFSNFGDSNIDFFIFLQAVDRNGTFILKSQL
                     MKRIHSRFTAEGIEINYPVRKLVLSDEVNFEEIIGSETTEM"
     gene            complement(5525..6370)
                     /locus_tag="QF838_10680"
     CDS             complement(5525..6370)
                     /locus_tag="QF838_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HJO61183.1"
                     /translation="MILTSQYPYSLDETLYSGQAFRWTPVLNGEVHEGILDGVRVRLT
                     QKEDGIHCSLGSRNLVDEGFIRKYLRIEDDLPRIYSQLSSDTYFTQAKNRYEGLHILR
                     QDPWECLITFICSANNNIPRIKSLVKGISESSGERIKDQFGHFYSFPGPVSISEFGEL
                     GLREIGLGFRAKYVAKVADIVGRNEIDLKSFRGLEYEEALIELLKFPGVGDKIANCVL
                     LFSMDKLQAFPVDIWIKRVLRENYLNDIDEAIADTKLRDWAQDYFGEFAGYANQYLFH
                     KRRLT"
     gene            complement(6372..>8817)
                     /locus_tag="QF838_10685"
     CDS             complement(6372..>8817)
                     /locus_tag="QF838_10685"
                     /inference="COORDINATES: protein motif:HMM:NF016724.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HJO61184.1"
                     /translation="DTLDLSYVIHPTQLDYSLTGLSLFLELDISNSHRAMADCENTMN
                     LFLNLLRSIKELKLQTFVQINNMAVKGSWDSQPVLQTLLSERLLSEKTNKNPTDSQQD
                     PPVDVQTEVQTKVQNFSDDLQEIFSDHGALAYFLTAFEERQEQLDMAMMVKETIENNR
                     TSIIEAGTGVGKSMAYLIPTIQYAKDNDKRVVISTNTINLQDQLMEEDLPMAAYVVKA
                     ADKSDPGNELRYSVLKGRRNYLCLKNFRNFASREQLDKTETILISKILVWLDTTLTGD
                     QAELNLSRAQHRFLWSRLNAEGAFMCGGYNGKCFLKQAREEANNSDLVVVNHSLLMAD
                     VKSGRSLLPEFGALVIDEAHHLEDQATQAFGFQVSHGLAGETLSSLRGRDSIFSNINA
                     LFTPLNMNTEDVSRLSRSSESVALSLTKLQDSFNTLFSFATEIMKQSTSHKSSFSPSD
                     KYRIKIGEEPPLWEDLCSLGRDIEEHVSAISKEFIAANRILEKYDNHRSVIEILGDIQ
                     NTQSSITEANNFIQHFLFERTENYVYWISHDNNPDYSTLSGAPIEVGDQLRDQLFDQN
                     FSVVITSATLSTDGSFQHINQRLGINPENQLLLGSPFPYEDLATVVMPTDIPDPREPY
                     YEEMVAKNIYQAAMAAGGRTIALFTSYSSLKSVFQLIKDKYSGEHLEILAQGISGTPR
                     QLIERMKKHPETVLLGTSSFWEGIDLRGDALQILVITRLPFDVPSDPIYAARAEKYSN
                     PFMEYALPSAIIRFRQGFGRLVRSNKDRGAVFILDSRVVNSRYGHRFIKALPKMEIQR
                     PKSEEVEDIVSSWLGI"
BASE COUNT         2442 a         1909 c         1713 g         2753 t
ORIGIN      
        1 agtattgata ccacaggttt tctaatggct cgaacgtgag acatacccat agacgcaaga
       61 actccgatgg tagccgcagt gattacatga ccctcttcca acactgaatc tgcttccttt
      121 atgtccttgc cttttactct tacattagtt ccaggggata cagatgtcct tataccaatt
      181 tcagtcaacc gaccagattt tgaaaaatct ttctccgtgg tatcttcaaa aggtattact
      241 gcatcacaat gttctggtac aggtgcccct gtcataatcc ttgatgcggc tccagtcttt
      301 aatggttttg tgggcaagga tcccgccggg atggtttcca atatttcaag catcactgga
      361 tgttcgtatg ttgccgttga tacgtctctc gacatgacag cgtatccatc cattgcagaa
      421 ttatccaagg gaggtatgtt tatttcagac ctaacttgat ccgatgtcac caatcctaac
      481 gcttcggtta ttaggaagtt ttgggcattg agaactccga attggctcaa tattttgtct
      541 ctagcctctt caacactaag catatcttcg tgatgatgtc cgtgatgatg tgtggccatt
      601 ggaatgtatc gctttatcgt ttgttttagg aatagcgttc tgaaattttg tattggtgtt
      661 cgagagattt gattatttga acgtgagctc tatctatctc cttcgcagac aaagtcttat
      721 catctgactg aaatatgatt ctcatggtta tggaatgttt tccgtccggt acctctcccc
      781 cttcgtattg atcgatgggg aacgaattag ccacaagttt gtttttactt atagtgctca
      841 aaatcgactg ggaatcgatt tctttatcgg ctattaaagc caaatcccta aatgaagatg
      901 gatatcgact aggttcagaa taatcaacga actgaacttc cgccaccttt tctatggaat
      961 ctagaaaaag acggaaaatt ataactggac tagaagaaag gtcataaaat tcactcagat
     1021 tggattttaa ttcaccgata tgtccgatca aaatttgatt tagaaaaata tcggccactt
     1081 tgcctgtttc ataagtgatt ccagcacttt tagtaaatga caaaacccgc tcaacccatg
     1141 aaatatgtgt tcgactacac cctttgcatc aaaaaagtcc atgacccgat ctgattcaga
     1201 ccataaatct gtggaattgc tcaatccagt gataaccccg acgatttcct ctttctctac
     1261 aggcatagaa tccgcttcat ctgtctccaa aggaagaaat actcggccga tttcaaataa
     1321 tcgaagccca gcttgctggt ccgttcgcct gtttctagac aaggtttcaa ggacattcgg
     1381 aactaaagtc ggtcggagat accttttgga agcatccatt ggattcgcga ttgttatagg
     1441 ttcctggtca ttagaaatcg aatcaaacaa gtttatggtc tcgagatctg tgacggaata
     1501 cgaaatggtc tcattcatac cagcagacac gagcctgtct ctcatatgct ccctcaccct
     1561 ctgaggattt ttggaaccta ggcttggaat ggaagaggac aaattgctgg taggcaaatt
     1621 atcatatcca tatatccgag catattcctc gatgatgtca tcttgtatcg aaatatctga
     1681 cctccagatg ggagggatta cattgataac atgggtcaca tcttcctcta ttttctgaaa
     1741 gccaagcctc ttaataatgt tccatatatc cttcatgaac accggagtac ccagtatttt
     1801 ttcaaatctg gcctcagtaa tttcaattgt tttaatatgg tgggatccgc taaaggtatc
     1861 gatgatgcct tcggccggaa ttcctccgca caattgacta atgagatacg tcgccctttt
     1921 caatgcaata ggtgcaagtt catgccgtat atctcgctca aaccggtacg aggcctctgt
     1981 attcaaacct agagcactcc tggtagccct cgtattggcg gcatcaaaat tagccgcctc
     2041 caaaaatata tctgtcgtat attcgtctat ttcagtattt acccctccca taattccggc
     2101 caggccaatg ggtttggaag tgtcagctat gactagcatc gggggagtga gggttctagg
     2161 ttgagaatca agagtttcca gccgttcaga tccaaaagcc tgcctaacta tgatttcatt
     2221 tccttccaac ttgttgaaat cgaatgcgtg gagcggctgc ccatattcca acatcacata
     2281 gttagttata tctactatgt tgttgattgg tctatgccct gatttagtta atacatcctg
     2341 aagccacact ggggaagggg caaccttaac attttcgata aatgatccag tgtatcttgg
     2401 gcagttgagg gagtcttgta ttgttacctt tatgcagtcc tctattttag tatccccact
     2461 ttgatacgaa gattccggtt ggataagatc cctggctagt aaggctgcta tttcatatgc
     2521 tgtaccaatt atggacaacc agtcaggtcg atttggagtt aattcagtgt caagaatcga
     2581 gtcagacagc aaatctttga ccgaagtacc agttatggat tcattatcta atactaatat
     2641 ccctccgctg tcttctccaa tacctagttc taaagcggaa caaaccattc ctttcgattc
     2701 aattcctcta atattggcgc cttttaaaat ttcatgtttc ccggatcttg gattaaagag
     2761 gttcgctcct tcttttgcga aaacaatttt ctgtccaatt tctaaattcg gggcaccgca
     2821 aactactttt gccttttctc cttttccgag gtctagcgtg ggaagtctga gtcggtctgc
     2881 attgggatgt gcctctaact cgatcacttg agccaccaca agcgagtcag aatcccattt
     2941 gtctccaatc tgctctatag acccgacttc taaacctgcc attgtcaaag aatgagccaa
     3001 atctgtgaca tcagagtcaa tgtctacata gttacgcagc caagaaagtg ggatcttcat
     3061 aagttagcct gttaagaaaa ctgttttaaa aatttcatat cattggaata aaaatgcctg
     3121 atatcgtcaa tcccatgctt taacattgct attcgttctg ctcccatacc ccaagcaaat
     3181 ccgctatata tttctggatc ataacctacc cctgccagaa ctttcgggtg aaccataccg
     3241 gctcccataa tttcaatcca acccgtattc ccacacactc ggcacccatc accttcacat
     3301 ttgaagcagt cgatcgacac ttcagctcct ggttcaacaa acgggaaaaa gtcacaccta
     3361 aatctggctt tcctcttatc gccaaaaatt cgtctagcga attcttctaa agtggctttt
     3421 aggttggcaa aagtaatatc tttatcaacc gctaggccct ctatctggca aaattgccat
     3481 tcatgagtgg cgtctgtcgc ctcgtatcgg taggtcttac ctgggaccac cactcgaact
     3541 ggaggggatt gtgattccat agtcctaatt tgcattggtg aggtgtgagt ccgcaaaagt
     3601 agtgggcggg aaccgtctgg taattcccta tcaatccata aactgttcca catatccctg
     3661 gctggatgct cttctggaat gttcaatttc tgaaaattgt atttgtctaa ttccacttcg
     3721 gggccttcaa tggtctggaa acccattgat tcaaacactt gacatatttc tctgacagcg
     3781 gctgtggttg gatggaggct gcccaatgaa atacttcggc ctggtaaggt cacgtccaaa
     3841 agttctggcc cgtttcctgt tacattattt tccttgtttt caattccaga tttctgactt
     3901 tcgtattttt ctgtcaaaag gttttttaag gaattagctt cgccaccagc ttttcttcga
     3961 ctatccacag ggagatcaga gattccacgt aacaaaccag taacttttcc gttacgtccg
     4021 agagtgttaa ttctccaagt ttcaagctca ttgagggtct caatttgaga aagggaaaat
     4081 aaggcgtcct tttctatttg gatccgtatt tcgaggtaag atttttcgtt cattaatcaa
     4141 gtaggtaatt catatgtctt aatacaataa aatataggcg caaattatac ctcacaacta
     4201 aaaatctatc ggataggaca gaaatatata aatagatatc cgttacgata gtttttttgt
     4261 tttccaaaat ttcataaatc attgatatat tttagctaca atttcgatac ataaatatac
     4321 ttctaacctt ataatatgca aaagaggcgt tagtttatta aaggtgtgta agagtggaaa
     4381 taagccaatt aactgattct gctttggtag gggccatagc tgcgggcctt atattggtcg
     4441 cctttgtaat tttagcaata tttttacggg tgactctagc tatcctgacc aaacgtgctg
     4501 ataagcagaa gaatgacaga ttgttattgg tgttcatagg ttctatcaag ggaccctcag
     4561 ttctactcgt gatttcttta gggcttctgt ttgcatattt cattattgcc aggatagatg
     4621 agggtatttt tacctcaata gctggaaccg gagaatatgc tttcagagtc tggcaagtct
     4681 ccgctatttt catggcaaca ttcacttttt ctcatctttc agatcggatg atcaaatggt
     4741 acctggccaa tgtggcagga aaaactgcaa caaccctaga tgacactttg ctccctattt
     4801 tcagaagggt gttgcctata actgtatatg cggttggcac attaattgct atcgacaatt
     4861 taggggtatc cattagccca atactggccg gtttgggtat cggtggattg gccgttgctt
     4921 tggctgttca gccaacgtta agcaatttct ttgcaggtac atatgttgta actgaaggcg
     4981 aattaaaggc tggtgatttc atagagttag acggaggacc ctctggttac gtagaaagtg
     5041 tcggctggcg cagcaccaaa attcgtagca gattcaacaa cctagtcatc ataccaaatt
     5101 cccggatggc tgaaagtata gttactaatt atttcagccc cacacctgcg atgaatgtaa
     5161 ttgtaacttg tggagtgagt tatgaatcag acctagaaca agttgaaaat atagccttgg
     5221 aagaggctga ggctcttgta aaggaatcca ggcattccat caaagatatg gaccctttct
     5281 ttggattttc taattttggc gattccaata tagatttttt tattttcctt caagctgtgg
     5341 atcgcaacgg tacttttata ctgaagagtc agctcatgaa gagaatacat tccaggttta
     5401 ctgccgaagg aattgaaatt aattatcctg ttcgaaaact tgtgctttca gatgaagtta
     5461 acttcgagga aattataggt tctgaaacaa cggaaatgta aattctggca aagaaccctg
     5521 atcgttaagt aaggcgcctt ttatggaaaa ggtactgatt agcgtatcct gcaaattcac
     5581 cgaagtaatc ttgcgcccaa tctcttaact ttgtgtcagc aatagcctca tcgatatcat
     5641 tcagataatt ttctcgcagg acacgtttta tccatatatc aaccggaaat gcttgaagtt
     5701 tatccattga aaatagtaaa acacagttag ctattttatc tcccactcca ggaaatttaa
     5761 gtagttcaat caatgcttcc tcgtactcca aacctctaaa actttttaag tcaatttcgt
     5821 ttctacccac tatgtcggct acttttgcca cgtacttggc gcgaaaacct aatcctattt
     5881 ctcttaaccc taattccccg aactcggata tagaaactgg tccaggaaaa gagtaaaaat
     5941 gcccaaattg atcttttatt ctttcgccgg aggattctga tatccctttt accaaggatt
     6001 taatcctcgg aatattattg tttgccgaac aaatgaatgt aataaggcat tcccaagggt
     6061 cctgtcttaa aatatgaagc ccttcatacc tattttttgc ctgagtgaaa taagtatctg
     6121 aagataattg ggaataaatt cgtggtaaat catcctctat ccgaagatat tttcttatga
     6181 atccctcatc aaccaaattc cggctgccaa gactgcagtg tattccatct tctttttgag
     6241 tcagccttac tctgacccca tctagaatcc cttcatgtac ctctccattt aatacggggg
     6301 tccaccggaa agcttgtccg ctgtaaagag tctcatcaag ggagtacggg tattgggagg
     6361 tcagtatcaa cttaaattcc aagccagctg gagactatgt cttcaacttc ttcagatttt
     6421 gggcgttgaa tttccatttt tggcaaagct tttatgaacc tgtgtccata cctgctattg
     6481 accacccggc tatctaatat gaaaaccgct cctcggtctt tattcgaacg gactaatctt
     6541 ccaaatcctt ggcgaaaccg aataatagcg ctaggaagag catattccat aaaaggattt
     6601 gaatattttt cagcccttgc cgcataaatc ggatcactag gtacatcaaa aggtaatctc
     6661 gttataacta ggatttgcaa ggcatctccc cggagatcta tcccttccca aaaagatgag
     6721 gttcctagca gcactgtctc aggatgtttc ttcattcgtt cgatcaattg cctcggagtt
     6781 ccagatatac cctgggccaa tatttccaaa tgctcaccag aatatttgtc tttaatcaac
     6841 tgaaatactg attttaagga agagtatgag gtgaacaatg caatggttcg ccctccggct
     6901 gccatggcgg cttgataaat atttttagca accatttctt cataataggg ttccctcggg
     6961 tcgggtatat ctgttggcat caccacagta gctaaatcct cataaggaaa aggggagcct
     7021 aacaacaatt ggttctctgg atttattcct aatctctgat tgatatgctg aaaagagcca
     7081 tcggtgctca aggtggcact agttattacc acagaaaaat tttggtcaaa cagctgatcc
     7141 ctaagttggt cgccaacctc aataggtgct cccgacaacg tggaatagtc aggattgttg
     7201 tcgtgcgata tccaataaac ataattttct gtccgctcaa aaagaaaatg ctggatgaaa
     7261 ttattcgcct cagtaatgga actttgagta ttttgaatgt ctcccaaaat ttcaataacg
     7321 ctacgatggt tatcatattt ttccaagatt ctgtttgcag cgatgaattc tttagatata
     7381 gcggaaacgt gttcttcaat atctcttccc aaagaacaca gatcctccca cagtggtggc
     7441 tcttcaccaa tcttaattcg gtatttatcc gaaggtgaaa aggatgattt atgtgaagtt
     7501 gattgcttca tgatttcggt ggcgaaagaa aatagagtat tgaaactatc ctgcaatttg
     7561 gttaatgata aggctacaga ctcacttgat cttgataatc gacttacgtc ctcggtattc
     7621 atgttgagag gtgtgaataa tgcatttatg tttgaaaata tcgaatcccg acctctaagt
     7681 gatgacaggg tttcaccagc aagtccgtga gatacttgaa acccaaaggc ttgagtcgct
     7741 tgatcctcaa gatggtgagc ttcatctatc accaaagccc caaactccgg gagtaaagaa
     7801 cggccagatt ttacatccgc cataagaagt gaatgattta cgacaactaa atccgaatta
     7861 ttcgcttctt ctcgggcctg ttttaaaaaa catttcccgt tatatccccc acacataaaa
     7921 gctccttcag cgttcagcct tgaccaaaga aacctgtgct gggctctcga taagtttaat
     7981 tctgcctgat ctcccgtcaa agttgtatcc agccacacta gaattttaga gattagaatg
     8041 gtttctgttt tgtctagttg ttctcttgag gcaaagttac ggaaattttt aagacatagg
     8101 tagttacgcc tgcctttcaa gacagaatac ctaagctcat tacccggatc agatttatcg
     8161 gctgccttaa caacatacgc cgccataggt aaatcttctt ccatcaattg atcttgcaag
     8221 tttatcgtat tagttgaaat taccactcgc ttatcgttgt ctttcgcgta ctggattgta
     8281 ggaatcaaat acgccataga tttaccgact ccggttcctg cttcaatgat ggaagttctg
     8341 ttattttcaa ttgtctcttt caccatcatt gccatatcca actgttcctg tctctcttca
     8401 aaagcagtca aaaaataggc cagagctcca tggtccgaga aaatttcttg caaatcatca
     8461 gaaaaatttt gtacttttgt ttgcacctca gtttgaacgt ctactggtgg gtcttgctgt
     8521 gaatcggtgg gatttttatt ggttttctca gacaataacc tttcagacag caatgtttgc
     8581 aaaacaggct ggctatccca tgatcctttt actgccatgt tgttgatttg gacaaaagtc
     8641 tgtaacttta attcttttat tgatcttagt aaatttaaga agaggttcat tgtgttttca
     8701 caatcagcca tcgcccgatg ggaatttgag atatccaatt ctaaaaacaa actaagccca
     8761 gtgagactgt aatctaattg ggtagggtga ataacatagg acaaatcaag agtgtcg
//
LOCUS       DASCNS010000304         7206 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002190520, whole genome shotgun sequence.
ACCESSION   DASCNS010000304 DASCNS010000000
VERSION     DASCNS010000304.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002190520"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="QF838_10690"
     CDS             <1..208
                     /locus_tag="QF838_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61185.1"
                     /translation="EKTKSPIRVPLTFLCDNRNSKDETEFIIVPLWSNICFAVLLWIS
                     DESGLSDAQDRLNILTRKIATRII"
     gene            complement(306..1421)
                     /locus_tag="QF838_10695"
     CDS             complement(306..1421)
                     /locus_tag="QF838_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61186.1"
                     /translation="MFRTKTLIIMTALMVSFLIPTVVFAETDTVTFGTAVVTNDQAVN
                     DSLVVSMSGVAPPAEGSAYNAVLVSSDGTKSLDLGELSVVQPVIQGVIQATGTINFVY
                     DSTSEGYDGEDLFATYSRIKLTNGSGGPTAYSDNVPVDAAVYINSAMSDINSIDAHLA
                     TAIAAAFSAQSSSDIDAMKTDIASAVANTSVISALADGLGANVTAAAAEDVEDSDMAE
                     GAAGVIASAANISSWVNAAKATADNDVASQTSISVASIFAGKVFNELSAARNGWDANT
                     DGTISSSTGEGGVVQARASAQQMATLTLEAKDLPEDEVATVSEDGTPVVVVVDSVLGL
                     GLPSVGERIIKDLMLLSFLFGIAMVGIGSVILSRNRT"
     gene            1684..2448
                     /gene="rsmG"
                     /locus_tag="QF838_10700"
     CDS             1684..2448
                     /gene="rsmG"
                     /locus_tag="QF838_10700"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HJO61187.1"
                     /translation="MFSVFKQDGSKPPEIRNPQRLHSGLSSLGITPQSTTISQLMFFH
                     HLLVDANSRVNLTKVLDWPEVIVRHYLDSLSVVMAVPSLFDGGENILDIGSGFGMPGI
                     PLKVMFPGNDLVMLDSVKKKMEFIDFAVKEMNLTNTMTVVGRAEDIAKTRKHRENYSV
                     VVSRAVATLPVLLELALPFCNVDGTFVAMKSSAVQSEIDRSENALNLLGGEIERVVNV
                     EDKLPFLPGKLVVIRKITETPDIYPRKAGIPKKRPL"
     gene            2445..3548
                     /locus_tag="QF838_10705"
     CDS             2445..3548
                     /locus_tag="QF838_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HJO61188.1"
                     /translation="MKFPTEFISLFFRRLSKKNDLGSDFAGKQNRVFGTVLPVRTALT
                     FLGAIFLIFFSFKVFRVDWASALDAFKTLDIVKYFIAFIAYYLSFLFRGLRWKIISFN
                     SSALDRSSDGDSILNHASTFQYSALVLSGWFVNSVAWLRMGDVYRAYCLGVISKLGFS
                     WGIGTLVAERFLDMIIVFLMILASLTMLSSGVKGQALDFLVIFAFIITAILTIFVSSL
                     AIADKRLVSWLPIFLRSHYSKFKIGVLSGFKNIFIVTFLGLAAWLMEITRLYFVTDSL
                     NIDMSIYFIILATMSNAVLSTFPTPGGIGIVEPGLTGIFIISVSSSDAASITLLDRSI
                     TYLSVLMFGGITFLLYQAWFQKKSKIISVGEVT"
     gene            3778..5031
                     /locus_tag="QF838_10710"
     CDS             3778..5031
                     /locus_tag="QF838_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61189.1"
                     /translation="MTLKEIHHTVKRPGRTQDHDSVEIPVAEELATVRGIVKKDNDVA
                     WYSRNYPIVSQNVEKAADREWTWTVYTGEMYEYRKHHDEVNKPLVEAAYVSGNKQPTG
                     NVAPAKDVTEEIREKARELGFGEVGFTKYDRKYTYKSKKAWVKYEHAICLALEQDYWQ
                     TQTIPSMEAEFAHFGTYEVEGALALDLADFIRDLGYRAQIHSPNDNSAAYIPMFVEAG
                     LGQLGANGQLLSPHFGNRSRLIIISTDAIVTYDEPVDYGIHKFCEMCQVCVNRCPGRA
                     LVKEKVFWRGVEKHKLMYDRCRPVMATYEGCGVCMLVCPVQRYGMAPVMEHFVETGEV
                     LGKGTDNLEGYEIRGKGYYGPGKLPSFDREFFEMPHGTKDEWLFTQFKQKIEEKGKVS
                     EDEKQEFVEKLGEILFEDSTFDAGL"
     gene            5059..5268
                     /locus_tag="QF838_10715"
     CDS             5059..5268
                     /locus_tag="QF838_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61190.1"
                     /translation="MTSRSTFEKEHIDGLFGELNTDYKGMPESEQLHRDAHLAIAYHD
                     SGRQIPDSIDPRVIALIAKYGPTGI"
     gene            5428..7119
                     /gene="ilvD"
                     /locus_tag="QF838_10720"
     CDS             5428..7119
                     /gene="ilvD"
                     /locus_tag="QF838_10720"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HJO61191.1"
                     /translation="MATNTKNLELLKHKSGEVSLGPDRAPARSMLRAVGLDDEDMEKP
                     FIAVANLASDVTPCNVHLTRIADKVKEGIRDAQGVPFMFGTITISDGISMGTEGMKGS
                     LVSREVIADSIETVCFTESMDGLAVVAACDKNMPGAMMSMARLNIPSIFVYGGAILPG
                     NYAGKDINVQDMYEAVGAFSTRQMSLEELIAMERVACPGEGACSGMFTANTMASAIEA
                     MGMSIPGAASIPAVDPRNLDVAHEAGKHLYYMLENGIKPRDIMTRKAFENGIRLVLAM
                     GGSTNAVLHLLAIAREAEVKLTIDDFDILSRETPYLTDLRPGGNYVMSDVDRSGGVQV
                     VLKELLDAGMLHGDAKAINGKTLEENLSDVHTKPDERVIFSVHNAKSSTGGLAILKGN
                     LAPEGAVIKVAGTKIEKHEGPARVFDGERSAFEAVTGGLIKDGDVVVIRYEGPKGGPG
                     MQEMLAVTGAIMGAGLGETTVLITDGRFSGATRGGSIGHVAPEAAVGGPIGLIEDGDI
                     INLDIEARDLSVKLTDNELEDRRSKWSPRKPNYERGVLAKYAKLVSSASVGAVTS"
BASE COUNT         2066 a         1364 c         1730 g         2046 t
ORIGIN      
        1 agagaaaacc aaatcaccta ttcgggtgcc actaacattc ttgtgcgata atcggaattc
       61 gaaagatgag acagaattca ttattgtccc gctctggtct aatatttgct ttgccgtctt
      121 gctttggatt tcggacgaaa gcggattatc tgatgcacaa gatagattaa atatactcac
      181 tagaaaaatt gctaccagaa taatatagaa attatctctt atcattatta ctcactacgg
      241 tagacaaaaa aaaaccccgt tcgagcgagc ggggtttttc catccaaaag gaatttatat
      301 ctggattatg ttctgttcct tgaaaggata acagagccaa ttcctaccat cgcgatgcca
      361 aacaggaatg acagtaacat aagatcttta attattcttt ccccaacgct gggtagtcca
      421 agtcctagta cactatctac gacaacaaca acgggagtac catcctccga caccgtcgct
      481 acttcatctt ctgggagatc cttggcttct aaggtcagag tagccatttg ctgggcactg
      541 gctctagcct gcacgacccc tccctctccc gtagaggaag aaatggttcc gtctgtattg
      601 gcatcccaac cgtttctggc tgcagaaagt tcattgaaaa ctttcccggc aaaaatactt
      661 gccaccgata tactagtttg agaggcaacg tcgttgtccg cagtagcttt tgcagcatta
      721 acccacgagg aaatgttagc cgcagaggct attaccccag cagctccttc agccatatcg
      781 ctatcttcaa catcttcagc agctgcggcg gtgacgttag ctcctaagcc atcggctaaa
      841 gcgcttatta ctgaggtatt agcaacggca ctagctatgt cagttttcat cgcatcaatg
      901 tctgatgaag attgcgcgga gaaggcggca gctattgctg ttgccaaatg cgcatctata
      961 ctgtttatgt cggacattgc cgagttgata taaacagcag catccactgg tacgttatcg
     1021 ctgtaggctg tgggcccacc tgatccgttc gtcaatttaa tccggctata ggttgcgaat
     1081 agatcttcgc cgtcatatcc ttccgaagta ctgtcgtaaa cgaaatttat ggttccagtc
     1141 gcctggatga caccttgtat gactggttgg acgacggata attctcctaa atccagagat
     1201 ttagtcccat ctgatgatac gagtaccgca ttatatgcac taccttcggc ggggggagca
     1261 acccctgaca ttgaaacgac taaggagtcg ttaacagcct ggtcattcgt tactacggcc
     1321 gtaccgaaag taacagtatc ggtttctgca aagactactg tcggtattaa aaacgacacc
     1381 atcaatgcgg tcattattat taatgtcttg gtcctgaaca tagcttacac gctgcctcct
     1441 tgaccaatga tttgactgaa tcagtcttta gttatttagt tacgcgtagt aataacacta
     1501 ccacaaacgt gtctcgcata gtagcacttc aggggtaaag attccaatag aaattgctat
     1561 aaaaatatcg ggaatgctac tttaggcaca tcgtacaata tgtttaagca ttcttgcttt
     1621 tagttaatta tggtcgaaac agccgggttt ttctaattaa taatatggga gtaggtggag
     1681 tagttgtttt ctgttttcaa acaagatggt tcaaaaccgc ctgagattag gaacccacaa
     1741 agattacatt ctgggctttc ttcattaggg ataactcctc agagcaccac tatctcccaa
     1801 ttgatgtttt tccaccattt actggtagat gccaactcca gagttaatct gactaaagtg
     1861 ttggattggc cggaggtgat cgtcagacat tatttggatt cacttagtgt tgtcatggca
     1921 gttccatccc tatttgatgg aggggaaaat atactggata taggttcagg attcggtatg
     1981 cccggcattc cattgaaggt gatgtttcct ggaaatgatt tggttatgtt ggactcagtt
     2041 aagaaaaaga tggaattcat tgattttgct gtaaaggaaa tgaaccttac caataccatg
     2101 acagttgtgg gtcgagcaga ggatatagca aaaaccagga aacataggga aaactattca
     2161 gtagtggtat caagagccgt tgcgactctt cctgtcctac ttgagttggc cttgccattc
     2221 tgcaatgttg acggtacttt cgttgccatg aaaagctcag cagttcaatc tgaaatcgat
     2281 cgatcagaga atgctttgaa tttattaggt ggagaaatcg aaagagttgt caatgttgag
     2341 gataaactgc cgtttttacc aggtaagctg gtagtcataa gaaaaataac agagactccc
     2401 gatatttatc ctagaaaggc tggaattcct aaaaagaggc cactttgaaa tttccgacag
     2461 aatttatttc tctatttttt agaaggttat caaaaaaaaa tgaccttgga agtgatttcg
     2521 caggtaagca aaatcgggtt tttggcactg tccttcctgt acggactgcc ctcacgtttt
     2581 tgggagccat ttttctgatt tttttttcct tcaaagtttt cagagtcgac tgggcctctg
     2641 ctttggacgc ctttaaaaca ctggacatcg taaaatattt cattgctttt attgcatact
     2701 atctcagttt tttattcagg ggactcagat ggaaaatcat ttctttcaac tcctctgccc
     2761 tagatcgatc ttctgatggt gactcaatcc tgaaccatgc ttccaccttt caatatagtg
     2821 ccctggttct atccggttgg tttgttaatt cggttgcatg gttaaggatg ggggatgtct
     2881 acagagctta ttgtttgggt gtgatctcaa aattggggtt ttcttgggga attggcactt
     2941 tagtggctga aagatttttg gatatgatta ttgtatttct aatgatttta gcttccctaa
     3001 cgatgctttc ctctggcgta aagggccaag ctttggactt tcttgtaatt tttgccttca
     3061 taataactgc aatcctcacg atttttgttt catctcttgc gattgcagac aaaaggttag
     3121 tttcttggtt acctattttt cttaggtcac attattcgaa attcaaaatc ggggttttaa
     3181 gcggctttaa aaatattttt atagtcacct ttttgggatt agcagcatgg cttatggaga
     3241 tcacgagatt gtattttgtt accgactctc tgaacattga tatgtcgata tattttatta
     3301 ttttagctac tatgagtaat gccgtcttat caactttccc aacaccgggg ggaataggga
     3361 tcgtagaacc agggctaaca ggaattttca taatatcggt ctcaagttct gacgctgcct
     3421 ctattacctt gctggacagg tcaataactt atttaagcgt gttaatgttt ggtgggatta
     3481 cattcttact ttatcaagcc tggtttcaaa aaaagtcgaa gatcatctct gttggtgaag
     3541 tgacttaaat tcggacattt gaaagactca tagatgttat ttaaagaggt gagccgaggt
     3601 gagccgaggt gagccccaga agtttgctat atagaggtga ttatttaagg ataaatgagc
     3661 agtgttttat tgacttgtcg acgtaacatt aatagacttc acttttgaag atagtcatta
     3721 ttgtgattgt gaattagtcg tcaagaattt ggaatacgtc tatggaggga atttgacatg
     3781 accttaaagg aaattcacca taccgttaag agaccaggta ggactcaaga tcacgattcc
     3841 gtggagattc cagtagcaga ggaactcgcg acagtacgag ggatagtgaa aaaagataat
     3901 gatgtagcgt ggtattcgcg aaattatcca attgttagcc agaatgttga gaaggctgcg
     3961 gatagggaat ggacgtggac ggtctatacc ggggagatgt atgagtaccg taaacatcac
     4021 gatgaagtca acaaacctct tgttgaggcg gcatatgtat caggcaacaa acagccaact
     4081 gggaatgtcg caccggctaa ggatgtgaca gaagagatca gagagaaggc aagagagctc
     4141 gggtttggtg aggtaggatt tacaaagtac gataggaaat atacctacaa aagtaagaaa
     4201 gcctgggtca agtatgagca tgcgatatgt ttagcacttg aacaggatta ttggcagacc
     4261 cagactatac caagtatgga agctgaattt gcccactttg gaacatatga ggtagaaggc
     4321 gctctagccc tggatcttgc tgactttatt agggatttag gttacagagc tcagatacat
     4381 agcccaaacg acaatagtgc tgcctacata ccaatgttcg ttgaggcagg gctgggacaa
     4441 cttggtgcta atggacagct actctcacca cattttggta acagatctag acttattatt
     4501 attagtacag atgcaatagt aacgtacgat gaaccagtcg attacggcat acataagttt
     4561 tgcgaaatgt gtcaggtctg tgtcaatcga tgccctggaa gagctttggt taaagagaag
     4621 gttttttgga ggggtgtaga aaagcacaaa ttaatgtacg atcgatgtcg acctgtaatg
     4681 gctacctacg aaggttgcgg ggtttgcatg ttagtttgcc ctgtacagcg ctatggcatg
     4741 gcccctgtga tggagcattt tgtcgagact ggagaagttc taggaaaggg gacggataac
     4801 ctggaaggtt atgaaattcg gggcaaagga tactacggac ccggtaaact tccctcgttt
     4861 gacagagaat ttttcgaaat gccccacggc acaaaggatg aatggctatt cactcaattc
     4921 aaacagaaga tagaggaaaa aggtaaggtc agcgaagacg agaaacagga attcgtggag
     4981 aaattgggag aaatcttgtt tgaagactct actttcgatg ctggattata aaggttttga
     5041 agtacaggtt atatcgacat gactagtagg agtacatttg aaaaggaaca tatagatggt
     5101 ctttttggtg aactcaatac tgactacaaa gggatgccag aaagtgagca gctgcataga
     5161 gacgctcatt tggcgattgc ctaccatgat tcaggaaggc aaatacctga ctccatagac
     5221 cctagagtga ttgcattaat agccaaatac ggaccaacag gtatctaaaa actgctcttt
     5281 gatggaagtc cctgctatcc caacgataaa ctgggatagc agggacttct catatataat
     5341 gccggcgcta aatatccccc aatacgataa tttaagatcg caaaacggcc taattgacct
     5401 gtgaacccaa atttaaggaa gtgaaaaatg gctaccaaca caaaaaattt agaattgttg
     5461 aaacataaat ctggagaagt tagtttgggc ccggatcggg ctcctgctag atcaatgctt
     5521 agggccgtag gactagacga cgaggatatg gaaaaaccat tcattgccgt tgctaatttg
     5581 gccagtgatg tcaccccgtg taatgttcat ttgacaagga tcgcagataa agtgaaagaa
     5641 ggcatacggg atgctcaggg agtgcccttt atgtttggca ccatcacgat tagcgacgga
     5701 atatcgatgg ggactgaggg tatgaaggga tccttggtaa gccgcgaagt gattgcagat
     5761 tctattgaaa ctgtgtgttt taccgaaagt atggacggtt tggccgtagt agcggcatgc
     5821 gacaaaaata tgccaggggc catgatgtct atggcgcgtc taaacatacc ttcgattttt
     5881 gtttatggtg gtgccattct tccagggaac tatgcaggca aggacataaa tgtccaagac
     5941 atgtacgaag cggttggggc attctctacg agacaaatgt cattagaaga attgatagcc
     6001 atggaacgcg tggcttgccc tggagaagga gcctgttccg gcatgtttac agccaacacc
     6061 atggcgtctg ctattgaagc gatgggcatg tcgatacccg gtgccgcatc tattcctgcg
     6121 gtggaccctc gaaatttaga tgtggctcat gaggcaggta aacatttata ttacatgctt
     6181 gagaatggga ttaaacctag ggatatcatg actcggaagg cttttgagaa tggtataagg
     6241 ttggtgttgg cgatgggagg gtctaccaat gcagttcttc atctattggc cattgcaaga
     6301 gaggcagagg ttaaattaac aatagatgat ttcgacattc tttcaaggga gacaccatac
     6361 ttaactgatc taaggcctgg aggaaattat gtaatgtctg atgtcgatag atccgggggt
     6421 gttcaagtag tattaaagga attgctagac gcaggaatgt tacatggtga tgccaaggca
     6481 attaatggta aaactctaga agagaatctt tctgatgttc atacaaagcc ggatgaaaga
     6541 gtaatcttct cagttcataa cgcaaaaagt tcaactgggg gactagctat tctcaaaggt
     6601 aatttagctc ctgaaggagc tgtcataaaa gttgctggga ccaagattga aaaacatgaa
     6661 ggaccagcaa gggtatttga tggggagcgt agtgcttttg aggctgtcac cggaggtttg
     6721 atcaaagatg gtgatgtggt tgtcataaga tacgaggggc ctaaaggcgg accagggatg
     6781 caggagatgt tggcggtgac tggggcaatc atgggtgctg ggttggggga aactacagtt
     6841 cttattaccg atggtagatt ctctggtgca actagaggtg gctccattgg tcatgttgct
     6901 ccagaggcgg cggtaggggg accaattgga ttgattgagg atggggacat aataaacttg
     6961 gacatagaag cacgggacct gagcgtaaaa ttgactgata atgaattaga agatagaagg
     7021 tcaaaatgga gcccaagaaa acctaattat gagagaggtg ttttggcgaa atatgccaag
     7081 ttagtgtctt cagcctcagt tggggctgtt accagctaat tctctaatta ctccaaacag
     7141 ctagtgatat aattcactag catcggtctc aatccattga ttggcgtcgt tattcccgca
     7201 gattaa
//
LOCUS       DASCNS010000305         3061 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: SAR202 cluster bacterium isolate Fu_MAG_135
            c_000002191214, whole genome shotgun sequence.
ACCESSION   DASCNS010000305 DASCNS010000000
VERSION     DASCNS010000305.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270956
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      SAR202 cluster bacterium (marine metagenome)
  ORGANISM  SAR202 cluster bacterium
            Bacteria; Chloroflexota; Dehalococcoidia; SAR202 cluster.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:02:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,100
            Genes (coding)                    :: 2,084
            CDSs (with protein)               :: 2,084
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 6 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="SAR202 cluster bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002191214"
                     /isolate="Fu_MAG_135"
                     /isolation_source="seawater"
                     /db_xref="taxon:2030829"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="QF838_10725"
     CDS             complement(<1..1082)
                     /locus_tag="QF838_10725"
                     /inference="COORDINATES: protein motif:HMM:NF028500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJO61192.1"
                     /translation="MRKILKQEVASEFNNSTVIGGIEAFLSSNVGMLPERFLEPLKGY
                     SIMNNQQRAYVVGELIRALTPDVKPNFSTPRKDIFLADSIESFKKSGRGWPVKKISES
                     LGLNTVKDLILHFPERHEDFSNIRKISDLISGETQSSELSVKSLQLKKSRNGKHDRVE
                     IEFSDTTGSMKVTFFGQQWILKDLKPGDKVMLSGKVEYEKGKPTYKNPVYDRINPRVT
                     KLHTGRLVPVYKLTQGIIVKTLRREIHAALTSALDQLDEYLPETLLSRTGLYPIKKAV
                     ARYHYPRNIQDFSRARRRIAFDELFLVQLAMRKKKLDWKNQVSGISLNQNRDILSLFI
                     STLPFKLTLAQSRCMDEICEDLSSGIP"
     gene            complement(1134..2798)
                     /locus_tag="QF838_10730"
     CDS             complement(1134..2798)
                     /locus_tag="QF838_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HJO61193.1"
                     /translation="MTDFNVTIADTFFGMFSSSVVFFKRHVEAINSLNVFPVPDGDTG
                     TNMYGTLHGMQSRMSSVSYNNLYELTDQLSQAALYEGRGNSGIILSQIFRGLSDHLSR
                     HTSDVDFELIAGAIENARLKAFKSVAEPAEGTMLTVLSDVAKKAEFLKRKNISIKNFF
                     RSLVEEAEKSVDRTPELLQILKESGVVDSGGYGLEVMLKGMSIFLEGNDPNNFPVLLR
                     LPKGDGLGVENLISDHFGSEDDFGYCTQFLLDTKTDMEGIENLFQGLGTSTVIVGDRD
                     IFRIHIHTVDPGKVLTKAIAIGSIGSVSIENMETQSQDILNSSKKEKPSVKIDLPGQT
                     ALMSIVNGDGILRMNNELGVDLAIDGGLDMNPSVEDIVTAVNQVRADSILLLTNDKNV
                     IPAANQAAELLTATIEVLPTETQVQGLECIFNFNPEISALQNKEIMGRLIPHIKTIAI
                     FKSSRSVKINGMEVGRLQPIVMTEGEIRKSGDTLDELFLEVIQEELGQHVEHVTVLVG
                     NGMNSNDIDRINKILDHNVENLEEDIIELHVGGQPHYDYLISVLSN"
     gene            2951..>3061
                     /locus_tag="QF838_10735"
     CDS             2951..>3061
                     /locus_tag="QF838_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013026.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HJO61194.1"
                     /translation="MATCEAPNCKIQVRTGNNVSHSKRRTRRTWSPNIHKA"
BASE COUNT          890 a          687 c          551 g          933 t
ORIGIN      
        1 ggaataccgc tagataaatc ttcacaaatc tcatccatgc acctagattg agccaaggta
       61 agtttgaatg gtagcgtgga aatgaatagg ctgagtatgt cacggttttg attcagtgat
      121 attccagaaa cttggttctt ccaatcaagt ttcttctttc gcatggccag ttgtactaaa
      181 aacaattcgt cgaaagctat tcttctacgg gctctactga aatcttgaat gtttctagga
      241 taatgatatc tagcaaccgc tttttttata ggataaagac ctgttcggct gagcaaagtt
      301 tctggaagat attcatctaa ttgatccaag gcagacgtaa gagccgcatg tatctctctt
      361 ctcaaagtct ttacaattat tccttgagtc aatttataca ccggcactaa tcggccggta
      421 tgtaacttgg taacccgtgg gttgattcgg tcataaactg gattcttgta tgtcggtttc
      481 cctttttcgt attccacttt cccactcaac atcactttat ctccaggctt gaggtcctta
      541 aggatccatt gctgtccgaa aaaggtcact ttcattgaac ctgtcgtatc cgaaaattca
      601 atttccaccc gatcatgttt tccgttacga cttttcttaa gttgcaagga tttcacggat
      661 aattcggatg actgggtttc cccgctgatc aaatcagaga ttttccgtat gttactaaaa
      721 tcctcgtgtc gttcagggaa atgcaaaatc aaatctttta ctgtgttcaa acctaaagac
      781 tctgatatct tttttacagg ccagcccctc ccgctcttct taaatgattc gatagagtcg
      841 gccaagaaaa tatcttttcg tggagttgag aaattaggtt tcacatcagg agttaatgct
      901 cgaatcaatt cccccactac ataagctcgt tgctggttat tcataattga atagcctttc
      961 aaaggctcca aaaatcgctc tgggagcatt ccgacatttg aagacaaaaa ggcctctatc
     1021 cctccaatca cagtggagtt attaaattcc gaagctactt cctgcttcag aatctttctc
     1081 aaggtttcca ccatcaaatt acgattgacc attgccgata tgccaactga taattaatta
     1141 cttaaaacag agatcaggta gtcataatga ggttgcccac ctacgtgcag ttcaataata
     1201 tcttcttcca aattttccac gttgtgatcc aagatcttat tgatccgatc aatatcattg
     1261 ctattcatcc cgttgcccac aagaactgtg acatgttcta catgttgccc taattcctct
     1321 tgaattacct ccaagaataa ctcatccaag gtatctccag acttacgaat ctctccttca
     1381 gtcatcacta ttggttgcaa tcgacccacc tccataccgt ttattttcac agacctggag
     1441 gatttaaata tcgcaattgt cttaatatgg ggtatcagtc ggcccattat ttctttgttt
     1501 tgtaaagcgg atatttctgg attaaaatta aaaatacatt ctagcccttg aacttgagtt
     1561 tccgtcggca acacctcgat ggttgcggtg agcaattcag cagcctgatt agcagccggg
     1621 atgacatttt tgtcgttagt taaaagtaaa atagaatctg ccctaacttg attgactgct
     1681 gtaactatat cttccacgct gggattcata tccaatcccc cgtcaatcgc cagatcaaca
     1741 cctagttcat tattcatcct tagaatcccg tccccattaa caatggacat aagcgccgtt
     1801 tgaccaggta aatcaatttt tacggaaggt ttttccttct tggaagaatt taaaatgtcc
     1861 tgggactgtg tttccatatt ttctatcgat actgacccta tacttcctat cgcaatagct
     1921 ttggtaagta ctttacccgg gtcaacggtg tgtatatgaa ttctgaatat atccctatcc
     1981 cctactatca cagtagatgt tcctaaacct tggaaaaggt tttctatacc ctccatatcg
     2041 gttttggtat cgagaagaaa ttgagtacaa tagccaaaat catcttcaga tccaaaatga
     2101 tcagaaataa gattctcaac ccccaaacca tctcccttcg gaagtctcaa taagacagga
     2161 aaattattag ggtcgttacc ttcaaggaaa atcgacatac ctttcaacat gacttcaagc
     2221 ccatatcccc ccgaatccac cactccactt tcctttagta tctgcaacaa ttcaggggtt
     2281 ctatctactg atttttccgc ctcttcaacg agggagcgga agaaattttt aatggagata
     2341 ttttttcttt taagaaattc cgccttcttt gcaacatctg acaatactgt gagcatagtc
     2401 ccttccgccg gctctgctac agatttaaat gccttgagtc tggcattttc aatagcgcct
     2461 gcgatgagtt caaagtcaac atcggacgtg tggcgggaca aatgatcaga caatcctcga
     2521 aatatttgag acaggattat cccactatta cctcttcctt cgtaaagagc cgcctgtgag
     2581 agctggtctg taagttcata aagattgttg tatgaaactg aggacatccg agattgcatg
     2641 ccatgcaggg taccgtacat gttggtgcca gtatccccgt caggaaccgg aaagacattt
     2701 agagaattta tggcttcaac atgtcgctta aaaaaaacaa cagaggagct aaacattcca
     2761 aaaaaagtat ctgctattgt tacattgaaa tccgtcatag atacaccaca acaaaaatat
     2821 gctggaagta taataaagga tatggtattt tttaacctag atttcttcca atgctaaggt
     2881 gatttagtga cttggaaatt gattcagcga cccataaaag atacgtaata aaattaccga
     2941 aaggcgaaaa atggctacat gtgaagctcc caattgtaaa attcaggtac gtaccggcaa
     3001 taatgttagc cattctaaaa ggcgaacgcg aaggacatgg agtccgaaca ttcacaaagc
     3061 c
//
