LOCUS       DASCPJ010000001         3391 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000000981, whole genome shotgun sequence.
ACCESSION   DASCPJ010000001 DASCPJ010000000
VERSION     DASCPJ010000001.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000000981"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="QGF01_00005"
     CDS             complement(<1..211)
                     /locus_tag="QGF01_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19322.1"
                     /translation="MIITIAGATKYIPIFSRITPMLTSLALLNYVDIDSEHEMLGYGI
                     GLILLNIGMYFVAPAIVIITIKKRLF"
     gene            complement(309..1238)
                     /locus_tag="QGF01_00010"
     CDS             complement(309..1238)
                     /locus_tag="QGF01_00010"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HJN19323.1"
                     /translation="MQTFNPKSSLSVYYELTKPKIWYLLVFTAFGAAITASNIYNIPI
                     SLATWALMLGGVAAGSAAANTLTNYRDRDIDAIMERTKDRPIPSGRINPPEKARNFGL
                     ILAAISLICAFGICFTTSFWNGILAGSFMAFGLADNILVYSYLLKRKSRSNIILGGFS
                     GGAPALIGYVAVTTTGLWDFGLIIAGLVFVWIPMHIWALTLHFKDDYKKVNVPMLTAV
                     QSEKTSARIIAGTTLMMVLFSIVPFFLTHDNGEPLMHEIYLYTAIASGVLMVILSFWV
                     VAKPSEKSSWVLFKFSSPYLAVLFIALMVDSAL"
     gene            complement(1268..1723)
                     /locus_tag="QGF01_00015"
     CDS             complement(1268..1723)
                     /locus_tag="QGF01_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJN19324.1"
                     /translation="MAKVDNLDLKILSELSDDAAISIPKLSKKIDVNTSVVYSRIKRL
                     LKRKMIERYTIEINDKELGYTVKSFTGINIDSKQRDSVINGLFEINGVREISEVTGRF
                     DILVTMHAKNLAEMHRLISEGIGKIQGIIGSESFVEMKRRKKQTPYMVD"
     gene            complement(1795..2868)
                     /gene="asd"
                     /locus_tag="QGF01_00020"
     CDS             complement(1795..2868)
                     /gene="asd"
                     /locus_tag="QGF01_00020"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HJN19325.1"
                     /translation="MKKRVAIIGVTGAVGQEFVQSLKNHPWFEVTQIAASERSAGKKY
                     VDAIRDDSGIVAWDVGGEIPDYIKDMPVLTIDEINVAELDLIFSAVESQAAIDIETKF
                     AKDLPVISTSSAYRYEEDVPILIPGVNDEQAELLEVQKKNRNWKGWVAPLPNCTTTGL
                     AITLKPLFDKYGAKKVLMTSMQAISGGGKTGVSAMGITDNILPYIPKEEEKVRKETRK
                     ILGKLKDGKIEDADIRVSCTCTRVPVIDGHTESVFVETEKEIDPALAKNTYDAYNKEI
                     SVAGLPSAPEDYYAFHEDPTRPQPRMEREVGDGMTTTIGRVEKEELFDNGIKYMLFSH
                     NKKMGSAKGAVLLAEMLYKKDKF"
     gene            2947..>3391
                     /locus_tag="QGF01_00025"
     CDS             2947..>3391
                     /locus_tag="QGF01_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJN19326.1"
                     /translation="MRIHPNKPAQSHIPASHGGKYGTKNPNPQVIDFSSNVNPLGCHP
                     GVKRYLKKQLDLISEYPDPSSSDLRTNLAWFTGVRKKQIVVGNGATEIIYNFCKTFLN
                     KKTPILIPIPTFSEYEVSAKLCECKISFFKTMNLSKNLQKFIMKIP"
BASE COUNT         1080 a          669 c          520 g         1122 t
ORIGIN      
        1 aaaataaacg ttttttgatt gtgattataa caattgctgg tgcaacaaag tacattccaa
       61 tatttagtag aatcagtcca attccatatc caagcatctc atgttctgaa tcaatatcaa
      121 cgtagtttag aagtgctaat gatgtcaaca ttggagtgat tctactaaat attggaatgt
      181 actttgttgc accagcaatt gttataatca caatcaaaaa acgtttattt taatttcagc
      241 aggattgttt tcataaataa ttatggatct tgatctagaa agaattttcg cttcgttgtt
      301 tgactaaatt aaagagcaga atctaccatt agggcaataa acagtactgc caagtatggg
      361 cttgaaaact tgaatagaac ccatgaagat ttttcacttg gttttgcgac tacccagaaa
      421 ctaagaatta ccattaaaac tccagatgca attgcagtgt acaggtagat ttcatgcatt
      481 aatggttctc cattatcatg agtaaggaaa aatggaacta tactgaataa taccatcatt
      541 aatgttgtac cagcaattat tctagcagaa gttttttctg attgaacagc agtaagcata
      601 ggaacgttta cttttttgta atcatcctta aagtgtaaag tcagtgccca aatgtgcatt
      661 ggaatccaaa caaacaccaa tcctgcaatt ataagaccaa agtcccaaag accagttgtg
      721 gttacagcaa cataaccaat caatgcaggt gcaccaccag aaaatcctcc taaaataata
      781 tttgatctgc ttttacgttt tagaagataa ctgtacacca aaatattatc tgctaatcca
      841 aacgccataa aagaaccagc caagatgcca ttccaaaaag atgtagtaaa acaaatacca
      901 aaagcacata tcaatgaaat tgcagcaagt atcaatccaa aatttcttgc cttctcagga
      961 gggttaattc gcccacttgg aattggccta tccttggtcc gttccataat agcatcgata
     1021 tctctatcac gatagtttgt tagggtatta gctgcagccg aaccagctgc tacacctcca
     1081 agcattaatg cccatgttgc aagagaaatt ggaatattgt aaatattaga agcggtgatt
     1141 gcagctccaa aagcagtgaa aactagaagg taccaaatct ttggtttagt taattcatag
     1201 tatacagaaa ggctagattt tgggttaaaa gtttgcatct atcattcacc ttctcacatc
     1261 cgttttatca atctaccata tagggtgttt gtttttttcg tctcttcatc tcaacaaaac
     1321 tttcggaacc tataattcct tgaatttttc cgatcccttc tgaaataaga cggtgcatct
     1381 cagcaagatt ttttgcatgc attgtaacta agatatcaaa cctaccagtt acttctgaaa
     1441 tttctcttac accattaatt tcaaataatc cattaatcac actatcccgt tgttttgaat
     1501 caatatttat tccggtaaat gatttgacag tatatcccaa ttctttatca ttaatctcaa
     1561 tagtgtatct ctcaatcatc tttcttttga gtagtcgttt tattctactg taaaccactg
     1621 atgtattcac gtcaattttt ttagaaagtt ttggaataga aatagctgca tcatcactaa
     1681 gctcggataa aatcttcaaa tccaaattat ccactttagc catctaaaac actcagaaaa
     1741 gctgtatttg gtggtctaat aaagctatta ttgaaatttt tacagaatta aggactaaaa
     1801 cttatctttt ttgtatagca tctcagctag gagaacagcg ccttttgcag atcccatctt
     1861 cttgttatgc gagaacagca tgtactttat tccattatca aacaactctt ccttttcaac
     1921 tctacctatt gttgtagtca taccatcacc aacttctctc tccattcttg gttgtggtcg
     1981 tgttggatct tcatgaaacg catagtaatc ttctggtgct gaaggcaatc ctgcaactga
     2041 gatttctttg ttgtaagcat cgtaggtatt ttttgctaaa gctggatcaa tttccttttc
     2101 tgtttcaaca aagactgatt cagtatgtcc atctatgacg ggaacacgtg tacatgtaca
     2161 gctaactcta atatctgcat cttcaatctt accgtccttt agtttaccta gaatttttct
     2221 agtctctttt ctcaccttct cttcttcttt tggaatgtat ggaagtatgt tatcagtaat
     2281 tcccattgca gatacaccag tctttccacc accggagatt gcctgcattg aagtcatcag
     2341 aactttcttt gcaccatatt tgtcaaatag tggcttgagt gtgatagcca aaccagtagt
     2401 tgtacaattt ggtagtggtg ctacccaacc tttccaatta cgatttttct tttgaacttc
     2461 aagcagttct gcctgttcat catttactcc tggaataagt attggaacat cctcttcgta
     2521 tctatatgca gatgaagttg agattacagg aagatctttt gcaaattttg tctcaatatc
     2581 tattgcagct tgtgactcta ctgctgagaa aattaaatct aattctgcaa cattaatctc
     2641 gtcaattgtt aaaactggca tgtcctttat gtaatcagga atctctccac caacatccca
     2701 agcaactatt ccactatcat ctcttatagc atcaacatac ttttttccag cagaacgttc
     2761 tgatgcagcg atctgggtaa cttcgaacca tggatggttc tttaaggatt ggacaaattc
     2821 ttgaccaact gctccagtta cgcctatgat ggctactctt ttcttcatgg atcattttca
     2881 ggttttcaat taataatcgt tagcaataac caaaacatac taaattagca atagataatc
     2941 tatgatgtga gaatccatcc aaataaacca gcacagtcac acattccagc ctcacatggg
     3001 ggtaaatatg gtacaaaaaa tcctaatcct caagtgattg actttagttc aaacgtcaat
     3061 ccattgggat gtcatccagg tgtaaagcgt tatttaaaaa aacaattaga tttgatctct
     3121 gaatatccgg atccaagctc ctcagattta cgtacaaatt tagcctggtt cacaggtgtt
     3181 agaaaaaaac aaattgttgt tggaaacggg gcaacagaaa taatctataa tttttgtaaa
     3241 acatttctaa ataaaaaaac gcctatttta attccaatac caacctttag tgaatacgaa
     3301 gtttctgcaa aactttgtga atgtaaaatt tcatttttta aaactatgaa tctttcaaaa
     3361 aacttacaga aatttattat gaaaattccc a
//
LOCUS       DASCPJ010000002         1817 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000015510, whole genome shotgun sequence.
ACCESSION   DASCPJ010000002 DASCPJ010000000
VERSION     DASCPJ010000002.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000015510"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="cobS"
                     /locus_tag="QGF01_00030"
     CDS             complement(<1..464)
                     /gene="cobS"
                     /locus_tag="QGF01_00030"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HJN19327.1"
                     /translation="MLKEVSSVFSFLTIIPSNNTDLGTVAKYMHIFPIIGIAIGLIIA
                     SIGFGLSFFLDPLIVSLLVVASIAVITGIHHTDGLADFADGLMTRGSKEKKRKAMKDL
                     STGSAGIVSVVLYIAGAIIALSLTDGYALFQAILLSEILAKFSMVLMASIGNS"
     gene            complement(457..1428)
                     /locus_tag="QGF01_00035"
     CDS             complement(457..1428)
                     /locus_tag="QGF01_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HJN19328.1"
                     /translation="MILESIVIVSFALLLDFLVGDPKTKYHPTAWIGKLIALLVPFAK
                     NSSAKRELIGGVLIVGIVITIVCMLLVGLDIGISLLTIDIVSLIVSVALGSILLKTTI
                     AIRGMQKHALAVADAVEKEDLDLARNHLSMIVKRDTKNLDKNHILSAVLESVSENTVD
                     GITGPLFYYAIFGLPGAFVYRAVNTIDSMIGYKTTLFKNVGWFGAKCDTILNYIPSRL
                     TGLVMILGALILGYNWKESFYIMKRDSTKLESPNAGYPMAALAGALGTKLEKMNYYTI
                     GNGSIEYTKSHIISAIRLMKVSSILFCGIVTIPIITCLSFLGWWIHA"
     gene            complement(1428..>1817)
                     /locus_tag="QGF01_00040"
     CDS             complement(1428..>1817)
                     /locus_tag="QGF01_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN19329.1"
                     /translation="MKLAEKTKSPVILITDIERGGSFGSIVGTMSLLEKKYQRVIKGF
                     VFNKFRGDLDILKPGFRKLKQNTGKPVFGTIPLTKFLLPEEDSITSNSKQLALNRKNL
                     KKIDSEIEKLSKVVKSSLNIRAIEKLL"
BASE COUNT          618 a          347 c          261 g          591 t
ORIGIN      
        1 gaattaccaa tacttgccat caaaaccatc gaaaattttg ctaaaatttc actaagtaat
       61 atggcttgga atagtgcata accatcggtt agtgataacg caatgattgc accagctata
      121 tacaatacaa cagaaacaat tcccgccgac ccagttgaaa gatctttcat tgctttgcgt
      181 tttttttctt ttgatcctct agtcataaga ccgtcagcaa aatctgccaa tccatcagta
      241 tgatggattc cagttatgac agcaatggat gcaacaacta aaagtgaaac aatcaatgga
      301 tctaggaaaa atgataatcc aaagccaatt gaagcaatta tcagtcctat cgcaattcca
      361 ataataggaa aaatatgcat gtattttgct actgttccca gatctgtatt attagaagga
      421 attattgtga gaaatgaaaa aactgaactt acttccttaa gcatgtatcc accatcctaa
      481 aaatgaaaga catgtaatga ttggtatagt cacgatgcca cagaataaaa ttgatgaaac
      541 tttcatcagt ctaattgcag aaattatatg agatttggta tattctatac taccattgcc
      601 aattgtataa taattcattt tttctaactt agttccaagt gcacctgcta gtgcagccat
      661 tggatatcct gcgttagggc tttctagttt tgtactatca cgtttcataa tatagaatga
      721 ttctttccaa ttatagccca aaatcaatgc acccaagatc attactaatc cagtgagtcg
      781 agatggaatg tagtttagta tagtatcaca cttagctcca aaccagccca cattcttgaa
      841 taaagttgtc ttataaccaa tcatagagtc tatggtgttt actgctctat acacaaatgc
      901 gcctgggaga ccaaaaatag cataataaaa taatggaccc gtaattccat caactgtatt
      961 ctcacttaca ctttctaaaa cagcagatag aatatgattt ttatccaaat tctttgtatc
     1021 tctttttaca atcatggaaa gatgatttct agccaagtct aaatcttctt tttcaacagc
     1081 atcagctact gctaaagcat gtttttgcat tcctctaata gcgatagttg tttttagtaa
     1141 aattgatccg agagctactg atactattaa tgaaacaata tcaattgtca ataaactgat
     1201 tccaatatct aaaccgacaa gtagcataca tacaatagtt ataacaatcc caacaatcaa
     1261 aactccacca attaattctc tttttgcaga actgtttttt gcaaatggca caagtaaagc
     1321 aattaatttt ccaatccaag cggttggatg atatttagtt tttggatcac ctaccaaaaa
     1381 atccagtaaa agtgcaaaac taactataac tatgctttca agtatcatta caagagtttc
     1441 tcaatagctc tgatattaag acttgacttt acaactttgc ttaatttttc aatctcagaa
     1501 tcaatctttt ttaagttctt cctattcaac gcaagctgtt ttgaattaga ggtgatagaa
     1561 tcctcctcag gaagtaaaaa cttggtaagg ggaattgttc caaaaacagg ctttcctgta
     1621 ttttgtttta gttttctaaa tccaggttta agaatatcta aatcaccacg gaatttgtta
     1681 aaaacaaaac ccttgatcac tctctggtat tttttttcta ataacgacat ggtaccaaca
     1741 atacttccaa acgagcctcc tctttcaata tcagtaatta aaattacagg tgattttgtt
     1801 ttctcggcaa gtttcat
//
LOCUS       DASCPJ010000003        14344 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000018947, whole genome shotgun sequence.
ACCESSION   DASCPJ010000003 DASCPJ010000000
VERSION     DASCPJ010000003.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000018947"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /gene="acs"
                     /locus_tag="QGF01_00045"
     CDS             <1..1572
                     /gene="acs"
                     /locus_tag="QGF01_00045"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HJN19330.1"
                     /translation="SLGIKSGDRVTIYLPMVPELIISMLACARIGAIHIVVFSGFSAI
                     SLKGRVEDSQSKIIITADGGYRKGKIINLKEIVDENAAIVDSVEHVIVLERIKNKITI
                     NKKDKFWTDLMTNASDVCSPEELDSNHPLYILYTSGTTGKPKGVLHGIGGYLTHIYST
                     YKWAFDIKDDDIYFCTADIGWVTGHSYVAYGPLLHGATQVMYEGAPDYPKPSRMWEII
                     QKYNVSIFYTTPTALRMFMKFGDHIPDSFDLSTLRLLGTVGEPINPEVWKWYFDVIGK
                     KKCPIIDTWWQTETGGMMITSLPGLESIVSKPGSATKPIPGVDIAVVDENGNETSPNT
                     KGSLVIRNPWPGMLLGLWKDDKKYTDVYWSKFDGMYYPGDYAIKDSDGYFWLLGRADD
                     VLKIAGHRIGTAELESSLVSHNDVIEAAVCGIPDEIKGESIIAFVILRENCTNSREKL
                     LIELRGVVRNEIGAIATPQQFYFVTKLPKTRSGKIMRRLLKSIVCGEKIGDTSTLEDK
                     TSVNEVQSAFNDQTL"
     gene            complement(1605..1730)
                     /locus_tag="QGF01_00050"
     CDS             complement(1605..1730)
                     /locus_tag="QGF01_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19331.1"
                     /translation="MQKRTMPICFTSKQYQSIVEYAKKNGMVNTSQAIEKLLSNI"
     gene            1813..2322
                     /locus_tag="QGF01_00055"
     CDS             1813..2322
                     /locus_tag="QGF01_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HJN19332.1"
                     /translation="MARKRQILVIGNNDDGCTPEVEKLAYDMGSKIAKSNSVLITGGL
                     GGVMRAASRGAHETGGLTVGIIPQNDFSLANEFCDIVIPSGMGLMRDFLNALSADGVI
                     VIGGGAGTLSEICAAYMYKKPIIALKNSGGTASKYADQFLDHRKNVQIIGVETADEAI
                     KCVLEQINT"
     gene            complement(2312..3451)
                     /locus_tag="QGF01_00060"
     CDS             complement(2312..3451)
                     /locus_tag="QGF01_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HJN19333.1"
                     /translation="MEIKNITVLGSGIMGHGIAQVYAMAGYNIVLRDIEQKFLDKAME
                     KIKWSLDKLVSKERISLEEESKILSRIKPIVDLKDAVHDSDLVIEAVPEIMDLKKKVY
                     AELDKVAGDQVIFASNTSTLPISEIANTLSRPDKFIGIHFFNPPQLMKLVEIIPGQKT
                     SDDILNLIINLVKSVKKTPVICRKDVPGFIVNRLFIPLVHEACYVMERQKIQQVEIDS
                     AVKFNLGFPMGIFELADYTGMDVIHKATIEMHLRDKKVILPHPKIEQLFNEKKLGQKS
                     GEGFYKYSDDKYERILLSEELGKKCNPTQIIANILNNAAWLVTNKASDVNEIEKAANL
                     GLNLKKPLFETANEIGMQKIVNELKKLSEQYGAFYEPDPLLLSMC"
     gene            3505..3672
                     /locus_tag="QGF01_00065"
     CDS             3505..3672
                     /locus_tag="QGF01_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19334.1"
                     /translation="MGLFGKKNSNKTKCDVCNLELPTPERLERHKKKAHGNVPEKKMD
                     GSSNAGEGGLW"
     gene            3705..3983
                     /locus_tag="QGF01_00070"
     CDS             3705..3983
                     /locus_tag="QGF01_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19335.1"
                     /translation="MEMNDSKKYGLMFLFTFLGAALIIGTVVFPFWNLIRADVYEEVE
                     IMLNERGMCYVDTSDNIPKTIDNCEYQIGDTVTVKFGEGLAYATIVSP"
     gene            4019..4429
                     /locus_tag="QGF01_00075"
     CDS             4019..4429
                     /locus_tag="QGF01_00075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HJN19336.1"
                     /translation="MDCIFCKIVNGEIKSRLIMESKNSVAFLDVFPVTKGHTLVIPKT
                     HYGKIQDMTQDDSADLFRVVCEVVPKIDKITGSTLLAIHNGKESGQEIPHVHVHLIPR
                     QSNDLAGPVHSMFKNRPNLSDDEFDQLHIMLKSS"
     gene            complement(4430..5158)
                     /locus_tag="QGF01_00080"
     CDS             complement(4430..5158)
                     /locus_tag="QGF01_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024763.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HJN19337.1"
                     /translation="MNRYKAIQTLSLESGATFDDAKYRYRKLALELHPDKNKNERNDD
                     KFKKITDAYHYLKKQNKMNNSKLINEKTANQEKSSMNTTKQTHWKTHSSDTQPTEDWT
                     SHTNNFEMDEEFWTLYEKSFWEDYEQNVFQKPKNNFEKMFWEDSEKNMEQMFKNRLEN
                     RKKFDHNLKIQVDKSLCIACCSCETIAPDVFVVDKISAINPKSKVHNQHGSHKGKIMD
                     AAKTCPTKAILVHQKDSKKKIYPL"
     gene            complement(5201..5297)
                     /locus_tag="QGF01_00085"
     tRNA            complement(join(5201..5245,5260..5297))
                     /locus_tag="QGF01_00085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5261..5263),aa:Ser,seq:cga)
     gene            5379..6401
                     /gene="fen"
                     /locus_tag="QGF01_00090"
     CDS             5379..6401
                     /gene="fen"
                     /locus_tag="QGF01_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HJN19338.1"
                     /translation="MGVDIKTLLIREKTKLESFSSKIIAIDAYNAIYQFLAIIRGPEG
                     MHLTDRKGRVTSHLTGLLYRNVNFLLMGIKPVYVFDGKPPSMKMAEIERRKKLKKAAT
                     VKYEKAKVEGDIETARKYAQQTTSMHDGMVQDSKHLLDLFGIPYVQAPADGEAMAAHL
                     NITGKAYAVASQDYDSLLYGARNLVRNFTNSGRRKLPNRNTYIDVEPEIINYQKTLDG
                     LSVTREQLIDIGILIGTDFNPDGFERIGPKTALKLIKEYSKLEDIPQIQEQLHTIQFN
                     KIREIFLQTNVSEPGNIEFHDIDYTGILGYLSRERDFSEDRVNASLNRLKKGLEKRSQ
                     TLEKWF"
     gene            6443..6739
                     /locus_tag="QGF01_00095"
     CDS             6443..6739
                     /locus_tag="QGF01_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19339.1"
                     /translation="MADIDHSKNVYLFIHGRVDLAEKSIDLLTSSGFSKEKIIMADPK
                     KAGSVDDYMAMLWMPPNPDHIKIQKITKVEEADDSGMIGVWKGVSKDDVSKIMF"
     gene            complement(6749..7096)
                     /locus_tag="QGF01_00100"
     CDS             complement(6749..7096)
                     /locus_tag="QGF01_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19340.1"
                     /translation="MSDIRFIIIGIVLVFSGIIVMSVFGSQFSELTIQAEFDKCFEYY
                     EDEPPREIDCDTGFQDKIFAFTPAAILIALGVFSIIKGIRGTWDQDVKPEDMVGPNNS
                     FDTHDEPKDSDDD"
     gene            complement(7127..7585)
                     /locus_tag="QGF01_00105"
     CDS             complement(7127..7585)
                     /locus_tag="QGF01_00105"
                     /inference="COORDINATES: protein motif:HMM:NF022072.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HJN19341.1"
                     /translation="MTKNNSEYAINHNANLKKSIIINAPPKKIWRKISDVVNLDWLVG
                     MKKCVSKSRNKSGIGTHRRLEFDDGSIINEYIVSWEDEHYFSYIAVDGLPLRGYHATI
                     SLTAYNQKSKVTWESYFNTEEMTQIEFREFCLFLESFYKNSLENLKKLLE"
     gene            7661..8944
                     /locus_tag="QGF01_00110"
     CDS             7661..8944
                     /locus_tag="QGF01_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19342.1"
                     /translation="MVFGWGKKKLDENEINVVPKEKHILLSDISNILEEISSVRTKTI
                     MAEVKTFRNKIDSNRKTLLKITKELDGDTLNTEEIDHHLRRLVDRGKSDVLSIIKKEC
                     TKNLIEINSFDDVKAFSETVTKMLKRVGDSLGRHSNVIHIFAKKYAKRLKDDLRVMQS
                     DNKEISVLVTNHIDLENKIEKITESVIEYEQFQESLSTLDVRKDNAEKNIQKFIEMNK
                     NIIDEIETLKNSKEYTAYLKIKEEINSFLSVRLEIKNQIDSQFANISRPMNKYIYISS
                     LDKSQKKLLENLINNPIDVLTDENKDGIIQILEAVIKNVKSESISVKNIEKSLNQLNQ
                     TISLVDEFIDKINNCSQSKIDAENRLSVFNYANLEKKELDLSKNKNNTSETESELESF
                     ELETGSITDSMSKSIKSIESILNQISAVHYVIALE"
     gene            8946..9356
                     /locus_tag="QGF01_00115"
     CDS             8946..9356
                     /locus_tag="QGF01_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HJN19343.1"
                     /translation="MLIKKNNHKRKVTMTEEARDGIISFCKMNHPNEGILILRGKSKR
                     GNVFVDGLVIPPFSETGADFAGFPHNPLPLDLSYVGIVHSHPAGSAEPSPTDLNNYFG
                     LVSMIVKSPYNDDDIFAWDSNGDEIPLEVLATEN"
     gene            9404..9730
                     /locus_tag="QGF01_00120"
     CDS             9404..9730
                     /locus_tag="QGF01_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19344.1"
                     /translation="MINYKHYLIFLFASLAVSIGLQMILPFPYGLGAALAIFIVFPLL
                     LRKRMMSRMKGSGQSGGSGFGLGGGGFFGQGQSSGVRYVCLTCNHRFKGGSCPRCGSK
                     MKRADF"
     gene            9745..10071
                     /locus_tag="QGF01_00125"
     CDS             9745..10071
                     /locus_tag="QGF01_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19345.1"
                     /translation="MPILALDEIRKKVIYQNTVDIWIAVCYEKGVDWNSTNGYKKFIA
                     YLLKSNLKMQKFPLCIKESGGDFERGHDKTKFGEELSESNDEVSTAYTIKLNDPAAEI
                     IRGFQF"
     gene            complement(10072..10545)
                     /locus_tag="QGF01_00130"
     CDS             complement(10072..10545)
                     /locus_tag="QGF01_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJN19346.1"
                     /translation="MYFLDDLKKTNILSSPVKKVKKNYFTGPIKIQEISTITKPNEHD
                     MYHVKFLKSSRTKLHYHSGAQLLIVTKGTGSLILYKKKDHATSKFQTKITKTINLKNG
                     DVAYIPAKTLHTHGAVKNEKIFSHIAINFYPHKNNAVKTVWFESDLKSNLFHKIS"
     gene            10622..11344
                     /locus_tag="QGF01_00135"
     CDS             10622..11344
                     /locus_tag="QGF01_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19347.1"
                     /translation="MTRDEYKIKTVVLRPSIDFDETEEIVENKKTSHFRAMLQKPKKA
                     DVHVHSIKLSYEAFLILSGKYSADYFRKVIHTISVGPTVKEVILGDTIFPIKRGKTIF
                     GKLGTKIKSSAKRKNQIDLELEEHVFLEEEQEIAFDHHGKEIKMPYKMSSRLIESYPK
                     RTLRNTKNVVKKPEITYDAAVQRLVVKLKKSVSIGTRSLEEKINVDEIIELYVPIYEA
                     RLVGPKKSVRLMRIDGIRKKIL"
     gene            complement(11345..12163)
                     /locus_tag="QGF01_00140"
     CDS             complement(11345..12163)
                     /locus_tag="QGF01_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJN19348.1"
                     /translation="MIFFSGNQKTEDLLYQAMSYMEKRQPKAAIPLFKKIIKQDPKNI
                     TALYNEGLALNQLKKYQDAITCFDQVTEIDPNDVAAVNNKGISLAEMGNTDGAFECYN
                     KSIEMDPKYAAAHYNKGVLHDKLLQHEESIQSLDNSLKCDSGNVNTAFYKGVVLGRMK
                     KHEEALNCFENIYRKHPKHMDAFFHKGIELAELGKHKKAIEIFEKLLQLHKDNVNVIY
                     AMARSNAAISNIDKALYLLEQAVKKDRKTIKRWAAEDEFFDSLLDEPKFRKLVK"
     gene            complement(12352..13668)
                     /locus_tag="QGF01_00145"
     CDS             complement(12352..13668)
                     /locus_tag="QGF01_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJN19349.1"
                     /translation="MIENDLKIYKKNTIKSSKLFKKSKKLHVNGVHHNIRFFEPYPFV
                     AKSSKGKFLTDVDSNKYVDYWMGHWSLILGHSPKNIMNDAKKQINDGWMYGTVNKNSI
                     ELSEKISKAVPVAEKIRYVTTGTEATMYAIRLARTATKRKTIAKIDGGWHGYTTDLLK
                     TVNWPFHNSESSGLVDEKYVKSLPYNNLHDSIKILKAVKNDLAAVIIEPVLGGGGGIP
                     ATKDYLSGIEEFCHRNGSLLILDEIVTGFRFRYGCMYETMKLDPDIVTLGKIVGGGLP
                     IGVITGKNELMSYANTSIFSKTNRAYIGGGTFSANPLTMITGNSTLSTLKNRGANLYK
                     KLNSFGSETRKMLDKKFDGEVITTGIGSLFLTHFVTNGDTEIKNASDVAKCNIKKLHD
                     YHFHMIANDNIFFLPGKLGAFSDMHTKSDITNIAKSTDRFLTKFKK"
     gene            13952..>14344
                     /locus_tag="QGF01_00150"
     rRNA            13952..>14344
                     /locus_tag="QGF01_00150"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
BASE COUNT         4780 a         2162 c         2476 g         4926 t
ORIGIN      
        1 tctttaggca taaaaagtgg tgatagagtg actatttacc taccaatggt accagaatta
       61 ataatatcta tgcttgcatg tgcaaggata ggtgctatcc acatagtagt tttttctgga
      121 tttagtgcaa tatcattaaa gggccgtgtt gaagattcac aatctaaaat tataatcacc
      181 gcagatggtg gttatcgtaa ggggaaaata atcaatctga aagagattgt agatgaaaat
      241 gctgcgatcg tagattcagt tgaacatgta attgttttag aaagaatcaa gaataaaatt
      301 acaataaata aaaaagataa gttttggacg gatttgatga caaacgcatc agatgtatgt
      361 agtcctgaag agttagatag taatcatcca ctttacatac tttacacctc aggcacaact
      421 ggaaaaccta agggtgtgtt acatggaatt ggcggatact tgacgcatat ttattcaaca
      481 tacaaatggg catttgatat caaagatgac gacatttatt tttgtactgc tgatataggt
      541 tgggttactg gacatagtta tgttgcatat ggtccacttt tacatggtgc aacacaagtt
      601 atgtatgaag gggcacctga ttatcctaaa ccatctagaa tgtgggaaat aattcaaaaa
      661 tataacgtaa gtatatttta cacaacacct actgcactga gaatgttcat gaagtttggt
      721 gatcatatac cagattcgtt tgatctatca acattacgtt tgcttggtac agtaggcgaa
      781 ccaattaatc ctgaagtttg gaaatggtac tttgatgtta ttggtaaaaa gaaatgtcca
      841 attattgata catggtggca aacagaaact ggaggaatga tgattacttc gcttccagga
      901 ttggaatcaa ttgtatcaaa acctggctca gcaacaaaac caattccagg tgttgatata
      961 gcagtagttg atgaaaacgg aaacgaaaca tccccaaata caaaagggtc attggtaata
     1021 agaaacccct ggccggggat gttacttggt ttatggaagg atgataaaaa atacactgat
     1081 gtttattggt caaagtttga tggtatgtac taccccggtg attatgctat aaaagattca
     1141 gatggttatt tttggttact cggtagagcc gatgatgtac tcaaaattgc tggtcataga
     1201 ataggtacag cagaacttga aagtagtctt gtctcacata atgatgtaat tgaagcggct
     1261 gtatgcggta tacctgatga aattaagggt gaatcaatca tagcatttgt tattttgaga
     1321 gaaaattgta caaactcacg ggaaaaatta ttgattgaat taaggggtgt tgtacgcaat
     1381 gaaataggcg caattgctac gccacagcag ttctattttg ttaccaaact gccaaagaca
     1441 cgcagtggta aaattatgag aagactacta aaatctattg tatgtggaga aaaaattggt
     1501 gatacaagca cattagaaga caaaacatct gttaatgaag tacagtcagc gtttaacgat
     1561 cagacactgt aatcatgaaa aaagaaaatt agtattattt atgactaaat attactgagt
     1621 aatttttcaa tagcttgact agtatttacc ataccattct ttttcgcata ctcaactatg
     1681 ctttgatact gtttactagt aaaacaaatt ggcattgttc gtttttgcat atactaatga
     1741 tttcatttaa tcttaatatc ctttcctata aaatttcaca aagtatttca tagattgttt
     1801 ggtataatct gtatggcaag aaagcgccag atcttggtga ttggaaataa tgatgatggg
     1861 tgtacgccag aagtagaaaa acttgcatat gatatgggtt ctaagattgc aaagtcaaac
     1921 tcagttttga taactggcgg tcttggtgga gtaatgcgtg cggcatctcg tggtgcacat
     1981 gaaactggtg gattaactgt aggaataata cctcaaaacg atttttctct tgcaaatgaa
     2041 ttctgtgaca tagtaattcc ctctggaatg ggtttaatgc gtgattttct aaatgcactt
     2101 tctgcagatg gtgttattgt aataggtggt ggagctggga cactttctga aatttgtgcc
     2161 gcatatatgt ataaaaaacc aattatcgca ttaaaaaatt ctggcggaac tgcatcaaaa
     2221 tacgctgatc agtttcttga tcataggaaa aatgtacaaa ttattggagt ggaaactgct
     2281 gatgaagcga taaaatgtgt tttagagcaa attaacacat agataacaat aatgggtctg
     2341 gttcataaaa cgcaccatat tgctcagata atttttttaa ttcattgaca attttttgca
     2401 taccaatttc atttgcagtt tcaaataatg gttttttaag atttagcccc aagttcgctg
     2461 ctttttcaat ctcatttaca tcactagctt tgtttgtaac aagccatgct gcattattta
     2521 gaatgtttgc gattatttgt gttgggttac attttttacc taactcttct gaaagtaaaa
     2581 ttctttcata tttatcatca gaatatttgt aaaatccctc tccacttttt tgacctagtt
     2641 ttttttcatt gaataattgt tcaatttttg gatgtggtaa aatgactttt ttatctctta
     2701 gatgcatctc tatggttgct ttgtgaataa catccattcc ggtataatca gctaattcaa
     2761 aaatacccat agggaaccca aggttaaatt tgaccgcaga atcaatctct acttgctgaa
     2821 ttttttgtct ttccatcaca tagcatgcct catgaacaag tggtatgaaa agtctattca
     2881 caataaaccc cggaacatct tttctacaaa ttactggtgt cttctttaca gatttcacaa
     2941 gattaatgat aagattgaga atgtcatctg aggttttttg accagggatt atttcaacaa
     3001 gtttcattag ttgtggtgga ttgaagaaat gaattcctat aaatttatca ggtctagaga
     3061 gggtgtttgc aatttctgaa attggtaatg tactggtatt tgatgcaaag ataacttggt
     3121 caccagcaac cttatctaat tccgcatata cttttttttt gagatccatt atttctggaa
     3181 cagcttcaat cactaagtca ctatcatgta cagcatcctt caaatctact attggcttta
     3241 ttcttgaaag gatttttgat tcttcttcta aggaaattct ttcttttgaa acaagtttat
     3301 ctaaactcca tttaattttc tccattgcct tgtctaggaa tttttgttcg atatctctaa
     3361 gcactatatt atatccagcc atggcataaa cttgagcaat tccatgaccc attattccag
     3421 atcctagaac tgttatgttt ttaatttcca taccgtccaa agaaattggc atcctttata
     3481 atatgtttct tgattattca aaacatggga ttatttggta aaaagaatag taacaaaaca
     3541 aaatgtgatg tgtgtaatct agaattgcct acacctgaac gattggagcg tcataaaaag
     3601 aaggctcatg gtaatgtgcc agaaaaaaag atggatggct cttcaaatgc tggtgaaggc
     3661 ggtttatggt gacttttcat aattaataca aatggtgaac tgttatggaa atgaatgatt
     3721 caaaaaaata tggcttgatg tttttattta cttttttggg tgcagctctc atcatcggaa
     3781 cagttgtatt tccattttgg aatttaatac gtgctgatgt ctatgaggaa gttgaaatta
     3841 tgttaaatga aagaggtatg tgttatgtgg atacttctga taacatacca aaaacaattg
     3901 ataactgtga atatcaaatc ggtgacactg taactgtaaa atttggtgaa ggacttgcat
     3961 atgcaactat tgtgagccct taacaatcat tttatatttt ataaaattct aacgaagtat
     4021 ggattgtata ttttgtaaaa ttgttaacgg agaaattaaa tctaggctga ttatggagtc
     4081 taaaaattct gttgcttttt tagatgtatt tccagttact aaggggcata cattagtaat
     4141 tccaaaaacc cattatggta aaatccaaga catgacacaa gatgatagtg ctgatctatt
     4201 tcgtgttgta tgtgaagtag ttcctaaaat tgataaaatt accggatcca ctcttttagc
     4261 tatacataat ggcaaagaaa gtggtcaaga aataccacat gttcatgttc atctaatacc
     4321 tagacaatca aatgatttag ctgggcctgt acacagtatg ttcaaaaaca gaccaaacct
     4381 ttcagatgac gagtttgatc aattacatat aatgttaaag agttcttgat tataatggat
     4441 agattttctt cttagaatct ttctgatgaa ctaatattgc ctttgttgga caggttttag
     4501 cggcatccat gatttttcct ttatgggaac catgctgatt atgtaccttt gatttaggat
     4561 taatggctga aatcttatct acaacgaata catcaggtgc aattgtttca caactacaac
     4621 atgctatgca tagactcttg tcaacttgaa ttttcaggtt atgatcaaat tttttcctat
     4681 tttctagtct atttttgaac atttgttcca tgtttttttc agaatcttcc caaaacattt
     4741 tttcgaaatt attttttggt ttttgaaata cattttgttc ataatcttcc cagaaagatt
     4801 tttcatataa tgtccaaaac tcttcatcca tctcaaagtt atttgtatga ctagtccagt
     4861 cttctgtagg ttgtgtatct gaagaatgtg ttttccaatg tgtttgtttt gtagtattca
     4921 ttgatgattt ttcctgatta gcagttttct cgtttattag tttagaatta ttcattttat
     4981 tttgtttttt taaataatga tacgcatctg tgattttttt gaatttatcg tcatttcttt
     5041 catttttatt tttatctgga tgtagttcta aggccaattt tctatatcta tatttggcat
     5101 cgtcaaatgt agcaccagat tcaagagata atgtttggat tgccttgtat ctattcacta
     5161 gtatctgtta tgtttgcata ttttaaaaag tattgaataa cgccgggaga gggatttgaa
     5221 cccccgagtg cgatgcacag acgctatcct gtatgagatt tcgaggcgtc cgccttacca
     5281 ggcttggcta tcccgacaaa gtaaatttat caaagatcct aatatagtca atactttgaa
     5341 agattaaata aaaaaataag gtaaaagaat ggtagatcat gggcgtagac ataaaaactc
     5401 tgttgatacg ggaaaaaact aaacttgaat cattctcttc aaaaataatt gcaattgatg
     5461 catataatgc aatttaccaa tttttggcga tcattagagg acctgaaggc atgcatctta
     5521 cggacagaaa aggtagagtt acaagtcatc ttactggttt gttatatcgt aatgtaaatt
     5581 tccttttgat gggaataaaa ccagtttatg tttttgatgg gaagccacct tccatgaaaa
     5641 tggctgagat tgagcgtaga aaaaaattaa aaaaagctgc gacagtaaaa tatgaaaagg
     5701 caaaagttga aggagacata gaaactgcca gaaaatatgc acagcagaca acatcaatgc
     5761 atgatggaat ggtgcaagat tccaagcatc tacttgatct ttttggaata ccatatgtgc
     5821 aggcaccagc tgatggagaa gcaatggctg ctcatctgaa cataacaggt aaggcttatg
     5881 ctgtggcaag tcaagattat gattctttat tgtatggagc aagaaatctt gttcgtaatt
     5941 ttacaaattc tggtagaaga aagctcccaa atcgtaatac ctacatcgac gtagaacctg
     6001 aaatcattaa ctaccagaaa actttggatg gattaagcgt tacacgtgaa caattgattg
     6061 atatcggaat tttaattggg accgatttca atcctgatgg ttttgagaga ataggtccaa
     6121 aaactgcact caaactaatc aaagaatata gtaaattaga agatatacca caaatacagg
     6181 aacaacttca tactattcaa tttaacaaga ttagagaaat cttcttacag actaatgtgt
     6241 ctgaacccgg taatatagaa tttcatgata ttgactacac cggaatactt ggttatcttt
     6301 ctagagaaag agatttttct gaagatagag taaatgcatc attaaataga ttaaagaaag
     6361 gattagaaaa aagaagccag actttagaga aatggttttg atattgaata tactaaaata
     6421 aaagaacaat attaaatcaa atatggctga tattgatcat tcaaaaaatg tgtatctttt
     6481 catacacggt cgtgtagatt tagctgaaaa atcaattgat ctattaactt ctagtggttt
     6541 ttcaaaagaa aaaatcatta tggctgatcc aaaaaaggct ggtagtgttg atgattatat
     6601 ggctatgtta tggatgcctc caaatcctga tcatataaaa attcagaaga ttacaaaagt
     6661 agaggaagct gatgattctg gaatgattgg agtttggaag ggtgtttcca aagatgatgt
     6721 tagcaaaatt atgttttaat aaatttaatt aatcatcatc agaatccttt ggttcatcat
     6781 gtgtatcaaa agaattgtta ggacctacca tgtcttctgg tttaacatct tgatcccatg
     6841 tacctcttat tccttttatt atagaaaata ctcctaatgc aataagaatt gcagctggtg
     6901 taaaagcaaa tattttatcc tgaaatccag tatcacaatc aatttctcta ggcggttcat
     6961 cttcataata ttcaaagcat ttatcaaatt cagcctgaat tgttaattca ctaaattggg
     7021 aaccaaaaac tgacataaca atgattccag aaaacacgag cacaatacca attataataa
     7081 agcgaatatc actcatatac taatttgata tattgaatat tttaggctat tctagcaatt
     7141 tcttaagatt ctctaatgag tttttgtaaa atgattctag gaataaacaa aattctctaa
     7201 attctatttg agtcatttct tcagtgttaa agtatgattc ccatgtaact ttggattttt
     7261 gattatatgc tgtcagtgaa atagttgcat ggtacccacg taacggcagt ccatctacag
     7321 ctatatatga aaaataatgt tcatcttccc aactaacaat atattcatta attatactgc
     7381 catcatcaaa ttctaaccgt ctgtgtgtgc caataccaga tttattacga gatttcgata
     7441 cacatttttt catcccaact aaccaatcca gattaacaac atcactaatc tttctccaaa
     7501 tttttttagg tggagcatta attataatag atttttttag atttgcatta tgattaatcg
     7561 catactcgga attattttta gtcataatcc tcataccgta tgataatttt taaattttct
     7621 agtttaatac atttaaaatt gtgattttat agtaaaatat atggtatttg gttggggtaa
     7681 gaaaaaatta gatgaaaatg aaattaatgt agtacccaag gaaaagcata tcctactttc
     7741 agatatttct aatattctgg aggaaattag cagtgttagg actaaaacaa tcatggcaga
     7801 agtgaaaaca tttaggaata aaattgattc aaaccgcaaa acacttctga aaattacgaa
     7861 agaattggat ggggatacac tgaatactga agaaatagat catcatttaa gaagattagt
     7921 tgatcgagga aaaagtgatg tattatcaat catcaaaaaa gaatgcacaa aaaatctaat
     7981 agagataaat tcatttgatg atgttaaagc gtttagcgaa actgtcacca aaatgttaaa
     8041 acgtgttggc gattcattgg gaagacattc taacgttatt catatatttg caaaaaaata
     8101 tgcaaaaaga ctaaaagatg atctacgtgt catgcaaagt gacaataaag agattagtgt
     8161 attagttacc aatcatattg atttagaaaa taaaattgag aaaattactg aaagtgttat
     8221 agaatatgag cagtttcagg agtcgttatc tacattagat gttcgtaagg ataatgctga
     8281 aaaaaatata cagaaattta tagagatgaa caaaaatatt attgatgaaa ttgaaacatt
     8341 aaaaaatagt aaagagtata cagcatattt aaaaattaaa gaagaaatta attctttcct
     8401 ttcagtgagg cttgagataa aaaatcaaat tgattcacag tttgcaaata tatctaggcc
     8461 catgaataaa tacatctata tctcatcact tgataaatca caaaaaaaat tattggagaa
     8521 tttaattaat aatcccattg atgtgttgac tgatgagaat aaggatggaa ttatccaaat
     8581 attggaagca gtaattaaaa atgtaaaatc agaatcgatt tctgttaaaa acattgaaaa
     8641 atcattaaac caacttaatc aaacaatttc tttagttgat gaatttattg ataaaatcaa
     8701 taattgtagt caatctaaaa ttgatgccga aaatagatta tcagttttta attatgctaa
     8761 tttggagaaa aaagaattgg atttgagtaa gaataaaaat aatacaagtg aaacagagtc
     8821 agaattagaa tcgtttgagc ttgagactgg ttcaataaca gactcaatgt ccaaatctat
     8881 taaatcaata gagtcaattc ttaatcagat ttccgctgtg cattatgtaa tagcattaga
     8941 ataaaatgtt gataaaaaaa aataatcata aacgtaaagt gacgatgacg gaggaagctc
     9001 gagatggaat aatatcattt tgtaaaatga atcaccctaa tgaaggaatt ctgattttgc
     9061 gtggaaaatc aaagcgtggg aatgtgtttg ttgatggttt agtcatacca ccattttctg
     9121 aaacaggtgc tgatttcgct ggattcccac ataatcccct tccactagat cttagttatg
     9181 ttggaatagt tcattcacat cctgcaggtt cggcagaacc atcacctact gacttgaata
     9241 actattttgg attggtatcc atgatagtga aatcacccta taatgatgat gacatatttg
     9301 catgggatag taatggtgat gaaataccat tagaagtgtt ggcaactgaa aactagtgat
     9361 cctgacaaaa ctttatatgg tttttttacc aatttttctt agtttgatta attacaagca
     9421 ttatttgata tttttgtttg catcacttgc agtaagtatt ggtttacaaa tgattttacc
     9481 atttccatat ggtcttggtg cagccttagc aatattcatt gtttttccat tactcctacg
     9541 aaaaagaatg atgtcacgta tgaaaggctc tggtcaatca ggcggttctg gatttggtct
     9601 tggtggcgga ggattttttg gacagggtca aagttctggt gtaagatatg tatgtcttac
     9661 ttgtaatcat agattcaaag gtggttcatg tccacgctgt ggttctaaaa tgaagcgcgc
     9721 tgacttttaa aaatttgtga tcagatgcct atacttgcat tagatgaaat aagaaagaaa
     9781 gtaatttacc aaaatacagt tgatatatgg attgcagtat gttatgaaaa aggtgttgat
     9841 tggaatagta ctaatggcta caaaaaattt attgcgtatt tattaaaatc aaatctaaaa
     9901 atgcaaaaat ttcctttatg tatcaaagaa tcagggggag attttgagag aggtcatgat
     9961 aaaacaaaat ttggtgaaga actttcagaa tctaatgatg aagtcagcac ggcatataca
    10021 attaaactaa atgatccggc agcagaaatt attagaggat tccaatttta actatgaaat
    10081 tttatggaat agatttgatt taagatcaga ttcaaaccat actgttttta ctgcattatt
    10141 tttatgtgga taaaaattta ttgcgatatg tgaaaatatt ttctcatttt tcactgcgcc
    10201 atgtgtatga agtgtttttg ctggaatgta agctacatca ccatttttca aattaatggt
    10261 ttttgttatt ttagtttgaa attttgatgt agcgtgatct tttttcttat ataaaattaa
    10321 actgccagta ccttttgtaa caattaacaa ttgtgcacca gaatggtaat ggagtttagt
    10381 tctagatgat ttgagaaatt tcacatgata catatcatgt tcatttggtt ttgtgatagt
    10441 agagatttct tgtattttta taggtccagt gaagtaattt ttcttcactt tttttacagg
    10501 tgacgataat atgttagttt ttttcaaatc atctagaaaa tacattttct atcaactaaa
    10561 tgtttgtgag ttatcatgaa aataacataa tattaaaaac tcttagtatc aatatagtgg
    10621 tatgactcgt gatgagtata agattaagac agttgtccta cgtccttcaa tagattttga
    10681 tgagacggaa gagattgtag aaaacaaaaa aacttcacat tttagggcaa tgctacaaaa
    10741 acccaaaaaa gctgacgtac atgtacattc tattaaacta tcatatgagg catttttaat
    10801 tttatcaggg aagtatagtg ctgattattt tagaaaagtt attcatacaa ttagtgttgg
    10861 accaactgtt aaagaagtga tcttgggtga tactatattt ccaattaaac gtggaaaaac
    10921 tatctttggt aaattaggta caaaaataaa atctagtgct aaaagaaaaa atcaaattga
    10981 tttagaatta gaagagcatg tatttttgga ggaagagcaa gaaattgcat ttgatcatca
    11041 tgggaaggag attaaaatgc cttacaaaat gagttcacga ttaattgaaa gttatccaaa
    11101 gcgtacatta cgaaacacaa aaaatgttgt aaaaaaacca gagattacat atgatgccgc
    11161 agttcaaaga ttggtagtta aattgaaaaa atctgtttca attggtactc gtagtttaga
    11221 agaaaaaatt aatgttgatg aaataattga actatatgta ccaatatacg aggcaagatt
    11281 ggtaggacca aaaaaaagcg tgcgtcttat gcgaatagat ggtataagga aaaaaatcct
    11341 ataattattt aacaagtttt ctgaattttg gttcatccaa caatgaatca aaaaactcat
    11401 cctcggcagc ccatcttttg atagtttttc gatctttttt caccgcctgt tctagtagat
    11461 ataatgcttt atcaatatta gaaattgcag cattacttcg tgccattgca taaatcacat
    11521 ttacattatc tttatgtaat tgtaatagtt tctcaaaaat ctctattgct tttttatgtt
    11581 ttcctaattc tgccaattca attcctttat gaaaaaatgc atccatatgt tttggatgtt
    11641 ttctataaat attctcaaag cagtttaatg cttcttcgtg ttttttcatc cttcccagca
    11701 cgactccttt gtaaaatgct gtatttacat tacctgaatc gcattttaat gaattatcca
    11761 aactttgtat tgattcttca tgttgtaata atttatcgtg aagcactcct ttattgtaat
    11821 gagctgctgc gtatttgggg tccatttcta tggatttatt atagcattca aacgcaccat
    11881 cagtattacc catttctgca agtgatattc ctttattatt tacagcagcc acatcatttg
    11941 gatcaatttc tgttacctga tcaaaacatg tgattgcatc ctgatatttt tttaactgat
    12001 ttagtgctaa tccctcatta taaagtgcag taatattttt tgggtcttgc tttattattt
    12061 ttttaaaaag tggtattgct gcttttggct gtcttttttc catatatgac atggcttgat
    12121 agagtaaatc ctccgttttt tgatttccac taaaaaatat caataaatca cacctagttt
    12181 aataccaaat ctttctcaac ttaaagattt gaactttttt attgcttttt ttgcaagttc
    12241 ttccatatct ccatgtggaa attgtgttgg atcatcaatt gctatatgct ctttatcttt
    12301 tttattaggt ttcatttttt ttattcttca tatatcatat atattattaa attatttttt
    12361 aaactttgta agaaatctat cggtggattt tgcaatatta gtaatatctg attttgtgtg
    12421 catatctgaa aatgcaccaa gtttgccagg taaaaagaaa atattatcat tcgcaatcat
    12481 atggaaatga tagtcatgta gtttttttat attacattta gctacatcgc ttgcattttt
    12541 tatctcagta tctccattag ttacaaagtg tgttaggaaa agtgaaccaa ttccagttgt
    12601 aattacttca ccatcaaatt ttttatctaa catttttctg gtttcagagc caaaactatt
    12661 taattttttg taaaggttag cacctctatt ttttagtgta ctaagtgttg agttcccagt
    12721 tatcattgtg agtgggttag ctgaaaatgt tccaccacca atgtatgcac gatttgtttt
    12781 tgagaatatt gatgtatttg cataactcat taattcattt ttaccagtaa taacaccaat
    12841 tggtaatcca ccaccaacta tttttcccaa cgtgactata tcaggatcaa gtttcattgt
    12901 ttcatacata caaccatatc taaatctaaa accagtcaca atctcatcta aaattaacaa
    12961 tgatccattt ctatggcaaa attcttcaat cccagataaa taatcttttg ttgctggtat
    13021 acccccacca ccaccaagaa ctggttcaat gataactgcc gctaaatcgt tcttcactgc
    13081 ttttagaatt ttgatcgagt catgtaaatt attatacggt aatgatttaa catatttttc
    13141 atcaacaagt ccactgcttt cagaattatg aaatggccag tttaccgttt tgagtaaatc
    13201 agtggtatag ccatgccaac caccatcaat tttggctata gtctttcttt tggtagcagt
    13261 tctagcaagt ctgatagcat acattgttgc ttctgtccca gttgtcacat aacgaatttt
    13321 ttctgcaact ggcacagctt ttgaaatttt ttctgataac tcaattgaat ttttattgac
    13381 agttccatac atccaaccat cattaatctg tttttttgca tcattcataa tattttttgg
    13441 tgaatgtcca aggatgagac tccaatgccc catccagtaa tcaacatatt tgttagaatc
    13501 tacatcagtt aggaatttcc cttttgagga ttttgccaca aatggatatg gttcaaaaaa
    13561 tcttatgtta tgatgtacac cattaacatg caatttttta gattttttaa aaagtttgga
    13621 cgattttata gtgttttttt tatatatctt taaatcattt tcaatcattt ttatccatca
    13681 ttttttctaa tatattactg atcgagctaa acatcttttt tttaaaattt ttatgtacag
    13741 tttatatgta tttgataaat ttcacttctt gcatacgtaa tgcaagagcg gcgcttgtga
    13801 tgaagtatgg tattggacca ttttatgatt tacgagcctc caattacgca tacaaaatga
    13861 ggatttgact gaattttcaa atctgagatg tgaagataag tgcattccgt caatttccaa
    13921 tattacaact aatagtactt caatgaatca gaatccggtt gatcctgccg gacctgactg
    13981 ctatcggatt gatactaagc catgcaagtc tttgtagtaa tacaaggcat acggctcagt
    14041 aacgcgtagt caacctaccc tatggatggg gataatctcg ggaaactgag cataataccc
    14101 aacagatcat caattctgga atggttgatg attcaaatga tttttcgcca taggatggga
    14161 ctgcggccta tcagcttgtt ggtgaggtaa tggcccacca aggcaattac aggtacgggc
    14221 tctgagagga gaagcccgga gatgggtact gagacaagga cccaggccct atggggcgca
    14281 gcaggcgaga aaactttgca atgtgcgaaa gcacgacaag gttaatccga gtgatttctg
    14341 ctaa
//
LOCUS       DASCPJ010000004         2222 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000059629, whole genome shotgun sequence.
ACCESSION   DASCPJ010000004 DASCPJ010000000
VERSION     DASCPJ010000004.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000059629"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="QGF01_00155"
     CDS             complement(<1..101)
                     /locus_tag="QGF01_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HJN19350.1"
                     /translation="MTTGYCVKCRSKREIKGATAVTMKNGKPVTKGS"
     gene            331..1158
                     /locus_tag="QGF01_00160"
     CDS             331..1158
                     /locus_tag="QGF01_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HJN19351.1"
                     /translation="MDVFADSSIDISLDLPIYTKSDIITIFGNIEHDDSLNLMIIDPK
                     ADIILSETVLLTQGEFTYNLQLNDYDLNTSGIYQISITYHDTNNSTEIEIKKEFFYDA
                     AQNVNPAGVDIERISESDQIPIFIIGIVIILGIFLFLARHAIFRRKNEYDDEDWQSKK
                     NRDYEKYHSEWMSDEQEFSGKEKKEIDENEFLKSLQGKNISDYYGILEIQKNASQSEI
                     KKQFRILAKKWHPDKKHGPDAEKKMAEINMAYGILSNQKNRKLYDQYYQSDDTDDKH"
     gene            1263..1820
                     /locus_tag="QGF01_00165"
     CDS             1263..1820
                     /locus_tag="QGF01_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19352.1"
                     /translation="MNLLDLAEQYYLITFGIVIGIGLLHGAILGRGIRSRFPSFKKHA
                     RIVSSILLVLFLVNAISSILKFAEPSKVTLSTLVIPTSPEEIFSLVINLLGIDAGLWT
                     VIGTFITVILIIFLKFADIHQIARYFIFTISCIVLIVALLGKFTDFIPTSFQIMLYAF
                     YQVGITIGIFLITRRKESDVLSEIK"
     gene            complement(1873..>2222)
                     /gene="tfb"
                     /locus_tag="QGF01_00170"
     CDS             complement(1873..>2222)
                     /gene="tfb"
                     /locus_tag="QGF01_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247877.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HJN19353.1"
                     /translation="NGINIRRKDVARCYRLIFRELDLKIPVADPVKGVSRIASIAGLG
                     EITKRKAIALLNKAKKIGIVAGKDPMGIAAAALYLACISSGGNKTQKEISIASGVTEV
                     TIRNRCAGLKKLL"
BASE COUNT          762 a          340 c          327 g          793 t
ORIGIN      
        1 caggaacctt ttgttactgg tttaccattt ttcatagtaa cagcagtggc acctttgatc
       61 tctctcttac ttctacattt cacacagtaa cctgttgtca tggtaagatt gagtattatc
      121 tcatattagc aatgacttgt gaactagatt tcacattttt gtgactctgt tttatataaa
      181 atcatcaaag aatatccaaa ctttgccagt tttcccctta aaacctagtt ttttaaaaat
      241 aatcaaataa aactaggaat attatcacag atatcaatgg attataaaca ctatttcatt
      301 atttttatga tatttttagc atttttacca ttggatgttt ttgctgattc atctatcgat
      361 atctcattag atcttccgat atacacaaaa tcagatatca taactatttt tggaaatatt
      421 gaacatgacg attcattaaa tcttatgata attgatccaa aagcagatat tattttgagt
      481 gaaacagttt tgctcacaca aggagaattt acgtataatt tacaattgaa tgattatgat
      541 ttgaatacga gtgggatata tcaaatctct atcacatatc atgatacaaa taattctact
      601 gaaattgaaa taaaaaaaga gtttttttat gacgctgccc aaaatgtaaa tccggcaggt
      661 gtggatatag aacgcattag cgaatctgac cagattccta tatttataat tgggattgtt
      721 ataattttag gcatatttct ttttcttgca cgccatgcta tatttaggag gaaaaatgaa
      781 tacgacgatg aagattggca atcaaagaaa aatcgtgatt atgaaaaata tcattccgaa
      841 tggatgagtg atgaacaaga gttttctgga aaagagaaaa aagaaataga tgaaaatgaa
      901 ttcctaaaat cattacaggg taagaatatt tcagattatt atgggattct tgaaatacaa
      961 aaaaatgcta gtcagtctga aataaaaaaa cagtttagaa tattggctaa aaaatggcac
     1021 ccagataaga aacatggacc agatgcagaa aagaaaatgg cagaaatcaa tatggcgtat
     1081 ggtattcttt caaatcagaa gaacagaaaa ctgtatgatc aatactatca aagtgatgat
     1141 acagatgata agcactagct tataatctaa ttagtgacta attttttatt ttcactctac
     1201 tgtaaaatta cattcttagc aatactaaat acattttgta ttttccatct tatgggaata
     1261 aattgaattt acttgactta gcagaacaat actatctaat cacttttggg attgttatag
     1321 gaattggttt actacatggc gcaattttgg gaagaggtat tcgttctcgt tttccaagtt
     1381 tcaaaaaaca tgcacgtatt gtttcaagta ttttacttgt attattttta gtcaatgcaa
     1441 tatcaagtat actaaaattt gcggagccat ctaaagttac attatctaca ttagttattc
     1501 ctacatcacc agaagaaata ttttcactag taatcaactt attaggaata gatgcgggat
     1561 tatggacagt tattggtaca ttcattactg tcattcttat tattttctta aaatttgcag
     1621 acattcatca aattgcacga tattttattt tcactataag ttgcattgtt ttaatagttg
     1681 cattacttgg aaaatttaca gattttatac caaccagttt tcaaataatg ttatatgctt
     1741 tctatcaagt tggtattacg ataggaatat tcctcataac taggcgtaag gaatctgatg
     1801 tcttatcaga aataaaataa tatctactgg aaaattgtaa ttggttttta gtatttgagt
     1861 ttaaaaataa atttatagta gttttttcaa gcctgcacat ctatttctaa tagttacttc
     1921 tgtgacacct gacgcaattg aaatttcctt ctgtgtttta tttccacctg atgaaataca
     1981 tgcaagatat aacgcagcgg cagctattcc cattggatcc tttcctgcaa ctattccaat
     2041 ttttttagct ttattcaata acgcaattgc ttttcgttta gttatttcac caagaccagc
     2101 tatactagca attcttgaaa caccttttac aggatctgct acaggtattt ttagatcaag
     2161 ttccctgaaa atcaatctat aacatctagc cacatctttt cttcttatat tgatcccatt
     2221 tg
//
LOCUS       DASCPJ010000005         4870 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000061004, whole genome shotgun sequence.
ACCESSION   DASCPJ010000005 DASCPJ010000000
VERSION     DASCPJ010000005.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000061004"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /gene="rplC"
                     /locus_tag="QGF01_00175"
     CDS             <1..414
                     /gene="rplC"
                     /locus_tag="QGF01_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HJN19354.1"
                     /translation="TTVDTAAITKGKGWEGPITRWGVKKKQHKSRKSVREVGSLGPIS
                     PQYVMYTVPRAGQRGFHQRIEYDKRIMIMSNTNDAEFKINPDGGFKHFGNVKGDFIIL
                     RGSVPGTYKRLVKLRTQIRNAPAKIIKPNILEVVV"
     gene            414..1226
                     /gene="rplD"
                     /locus_tag="QGF01_00180"
     CDS             414..1226
                     /gene="rplD"
                     /locus_tag="QGF01_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HJN19355.1"
                     /translation="MKVPILTTDGKKDGDVDLPLIFSTEFRKDLIHKAFTNLNSHKFQ
                     PQGRHPTAGMDVVARSNDPPTGQGRSRIAKMRGGGGGRQGEAGGVASVRGGRQAHPPT
                     VKKVIFKKLNQKENKLALCSAIAATKSKEIIESRGHKTKNISNFPMIVSDKIESVLKT
                     NEMVKILRSLNLIQDIERLKSRKPRTGKSLLRGRGTKIGKSVLFVVSKAEKLSKACKG
                     IPGVDVQTTKNLSVLDLAPGADPIRLTVYSTTALKEIGEIKSPHLELMVSMN"
     gene            1223..1489
                     /gene="rplW"
                     /locus_tag="QGF01_00185"
     CDS             1223..1489
                     /gene="rplW"
                     /locus_tag="QGF01_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HJN19356.1"
                     /translation="MKTETANKIILNPYITEKTFELVENDAKICFIVTNKSTKIMISE
                     AIETLYHEKATSVNTARTLYGKKAFVQFETVDKARDLATKIGML"
     gene            1530..1925
                     /locus_tag="QGF01_00190"
     CDS             1530..1925
                     /locus_tag="QGF01_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HJN19357.1"
                     /translation="MVKEFAYNGLSEKELNGLSLEKLLAVFPARARRSLTRGINDNKR
                     KLIKDIKTAKTDKTSTSIKTHIRDLIILPYMVGVTVNVFSGKEFMPVNITTQMVGHYL
                     GEYVITNKRVSHGAPGVGSSRSSLYVPLK"
     gene            1931..2389
                     /locus_tag="QGF01_00195"
     CDS             1931..2389
                     /locus_tag="QGF01_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HJN19358.1"
                     /translation="MGRFSYAFQDFDKTKHVRAAIREKGISHKHAREIGISIKGLSIE
                     KSRDYLLSVIKLERSIPFKRYKNQVGHKSDPGTMSGRYPQKAATEILKLLDNLESNAE
                     YKGMDLDRLKIINATVHKGRLVKRFIPRAQGRATAKNNVLTHVELVAQEK"
     gene            2392..3192
                     /locus_tag="QGF01_00200"
     CDS             2392..3192
                     /locus_tag="QGF01_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HJN19359.1"
                     /translation="MTQVKTLIKDNYNLMLLNDFLRKEIKEAGFSKVEVAKTPTGTRI
                     TLYVTKPGIVIGRKGTGIKELTSRLETDFQLKNPQIAVEEIKKPELSPNVMCNRMASH
                     IQRGTAFRRVTMWTLQQIMESGAMGVQITISGKLRGDRSAFEKHSLGILPRAGHHAEI
                     VVEEDIAHVETPMGLIGIRIRIAQKEKIIPEFELNEKISKRKSDAKADVESDAKADVE
                     SDAKADVESDAKADVESDAKSESEEIVIEEEKIKKMKETLEEEEAEFK"
     gene            3192..3395
                     /gene="rpmC"
                     /locus_tag="QGF01_00205"
     CDS             3192..3395
                     /gene="rpmC"
                     /locus_tag="QGF01_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HJN19360.1"
                     /translation="MVKLGSKTIRELNEKDLRDKLEEMRSELSKLRVESSKGTLRKES
                     GKLKPVRKNIARMLTRINELNKE"
     gene            3392..3658
                     /locus_tag="QGF01_00210"
     CDS             3392..3658
                     /locus_tag="QGF01_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HJN19361.1"
                     /translation="MITLENLKLHEMIGMQTKIIKSTNPQLNNIKGRIIDETKSTFTL
                     STTNGLKKFPKQQNQWEFQLNGVKISLNGRMISKKPEDRIGMKK"
     gene            3655..3984
                     /locus_tag="QGF01_00215"
     CDS             3655..3984
                     /locus_tag="QGF01_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HJN19362.1"
                     /translation="MTRNIGLPIKNPKKKADDGDNKNPFNGSLSIRGKIFEGIVIKSM
                     AKNTVILEKEAPIYFEKFKRYGRSKNRIHAHVPSNLDVNDGDRVMAAECRPLAKSVSF
                     VVVEVKK"
     gene            3984..4415
                     /locus_tag="QGF01_00220"
     CDS             3984..4415
                     /locus_tag="QGF01_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HJN19363.1"
                     /translation="MGGARSKASKGVVEFRPYVTRVIPVGAQIVCADNTGAKILEIVN
                     VHKYKTRVSRLPSAAVGDFCNVVVKKGPAELRKQVYGAVIIRQKYAIRRLNGVRVSFE
                     DNAAVLITPEGEVKGTDIKGPVSAEAAEKWPRIANLASVVI"
     gene            4417..4866
                     /gene="rplX"
                     /locus_tag="QGF01_00225"
     CDS             4417..4866
                     /gene="rplX"
                     /locus_tag="QGF01_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HJN19364.1"
                     /translation="MKPGKVRNQQIYRATNQVRNKQLGASLSKQLRQKYGRRHIRILD
                     GDVVKVTRGEYKGINGKITKISLDGNRVAIEGIKKEKIKGDKVDILIHTSNLLITSLK
                     TDDKWRTKILEAKTKPTEAKTKPTEAKTKPTEAKTKTKSSNKKEDEK"
BASE COUNT         1954 a          668 c          944 g         1304 t
ORIGIN      
        1 acaactgttg atactgcagc aattactaag ggtaaaggct gggaaggtcc tatcacacga
       61 tggggagtaa agaaaaaaca acataaatca agaaaaagtg taagagaagt tggttccctt
      121 ggtccaatat ctccccaata tgttatgtat actgttccaa gagccggaca acgtggattc
      181 catcaaagaa tagaatatga taaaagaatt atgataatga gtaacacaaa tgatgctgaa
      241 tttaaaatta atccagatgg tgggtttaag cattttggaa atgttaaggg tgactttata
      301 atattacgtg gatctgttcc tggtacatat aaacgactag taaaactaag aacacaaata
      361 cgaaatgctc cagcaaagat aatcaaacca aacatattgg aagttgttgt ttgatgaaag
      421 ttccaatact tacaacagat ggaaaaaaag atggagatgt agatttacca cttatatttt
      481 caactgaatt tagaaaagat ttaattcata aagcatttac aaatctaaat tctcataaat
      541 tccaaccaca aggaagacac ccaactgctg gtatggatgt agtggcaaga tcaaatgacc
      601 cacctacggg tcaaggaaga tcaagaattg caaaaatgcg tggtggtgga ggcggtcgtc
      661 aaggtgaagc tggtggcgta gcatctgtaa gaggcggtag acaagcacat ccacctactg
      721 taaaaaaagt aatttttaaa aaattaaacc aaaaagaaaa taaacttgca ttatgttctg
      781 caattgcagc aacaaaatca aaggagatta ttgagtctag aggccataaa actaaaaata
      841 tatctaattt cccaatgatt gtttcagata aaattgaatc agtattgaaa actaatgaaa
      901 tggttaaaat tttacgctct ttaaatttga tccaagatat tgagcggcta aaatcacgaa
      961 aaccacgtac cggtaaatcc ctactaagag gcagaggaac aaaaattgga aaaagtgttt
     1021 tgtttgttgt ttcaaaagca gaaaaattat caaaagcatg taaagggatt cctggtgttg
     1081 atgttcaaac aacaaaaaat ttaagcgttc tagatttagc cccaggtgca gacccaatta
     1141 gattaaccgt ttattcaact actgcattaa aagaaattgg tgagataaaa tcccctcatt
     1201 tagaattaat ggtgagtatg aattgaaaac agaaacagcg aataagataa ttcttaatcc
     1261 atacatcact gaaaaaacat ttgaattggt tgaaaatgat gcaaaaattt gtttcatagt
     1321 aacaaacaaa tctacaaaaa tcatgatatc ggaagcaatt gaaacacttt atcatgaaaa
     1381 agccaccagc gtaaatacag caagaacatt atatggtaaa aaggcctttg tgcaatttga
     1441 aactgttgat aaagctagag atctagcaac taaaatagga atgctctaat atggttcaaa
     1501 aattaaaaca acaagaatgg tgatattgat tggttaaaga atttgcatat aatggattgt
     1561 cagaaaaaga gttgaatggg ttatcattag aaaaactact tgctgtattt cctgctagag
     1621 ctagaagatc tttaactaga ggaattaatg ataataaaag aaaattaatc aaagatatta
     1681 aaactgcaaa aacagataaa acatccacat caattaaaac acatattcgt gatcttatta
     1741 ttctaccata catggtagga gttactgtaa acgttttttc aggaaaagaa ttcatgcctg
     1801 ttaacataac cacacaaatg gttggccatt atcttggcga atatgttatc acaaataaac
     1861 gtgtaagcca tggtgcacca ggagttggtt catcaagatc aagtttgtat gttccactaa
     1921 agtgagaaat atgggtagat tttcatatgc atttcaagat tttgataaaa ctaaacatgt
     1981 tcgtgcagca atcagggaaa aaggaatttc tcataagcat gcacgtgaaa taggaatatc
     2041 aattaaagga ttatcaattg aaaaatctcg tgactattta cttagtgtaa tcaaattgga
     2101 acgaagtatt cctttcaaac gctacaaaaa tcaagttggt cataaatcag atcctggtac
     2161 aatgtcaggt agatacccac aaaaagcggc gactgaaatt ttgaaattgt tagataattt
     2221 agaatcgaat gcagaataca aaggaatgga tttagataga ttaaaaataa ttaatgcgac
     2281 tgtgcataaa ggcagattag taaaacggtt tattccacgt gcacaaggta gagcaacagc
     2341 aaaaaataat gtgttaacac atgttgaact agttgcacag gagaaataaa aatgacacag
     2401 gttaaaactc tcataaaaga taattataat ttgatgcttc taaatgattt tctgagaaag
     2461 gaaattaaag aagctggatt ttcaaaagtt gaagttgcta aaacacctac tggtaccaga
     2521 ataactctat atgttactaa acctggaatt gttataggta gaaaaggaac tggtattaag
     2581 gaattaacaa gtagattaga aacagatttt caattaaaaa atccacaaat tgcagttgaa
     2641 gagataaaaa aaccggaatt gtccccaaat gttatgtgta atagaatggc atctcatatt
     2701 caacgtggaa cagcatttag aagggtaact atgtggacat tacaacaaat tatggaaagt
     2761 ggggctatgg gtgtacaaat tacaatttca ggtaaattac gtggtgatcg atctgcattc
     2821 gaaaagcatt cattaggaat tctaccaaga gctggtcatc atgcagagat agttgtcgaa
     2881 gaagatattg cacatgtcga aacaccaatg ggattaattg gtattagaat cagaattgca
     2941 caaaaggaga aaatcatccc tgaatttgaa ttaaatgaaa aaatttcaaa gaggaagtct
     3001 gatgcaaagg ctgatgtaga atctgatgca aaggctgatg tagaatctga tgcaaaggct
     3061 gatgtagaat ctgatgcaaa ggctgatgta gaatctgatg caaagtctga atcagaagaa
     3121 atagtaattg aagaagaaaa aatcaaaaaa atgaaagaaa cattagaaga agaggaggcg
     3181 gaatttaaat aatggttaaa ttgggttcaa aaacaataag agagttaaat gaaaaagatt
     3241 tgcgtgataa gttagaggag atgcgttctg aactttcaaa attacgtgtt gaatcgagca
     3301 aaggcacatt acgaaaagaa agtggtaaac ttaaaccggt acgaaaaaat attgccagaa
     3361 tgcttactag aattaacgag ttgaataaag aatgattact ttagaaaatt taaaacttca
     3421 tgagatgatt ggtatgcaaa caaaaattat aaaatcaaca aaccctcaac tcaataatat
     3481 taaagggcgt attattgatg aaactaaatc aacttttact ttatctacaa caaacggtct
     3541 gaaaaaattt ccaaaacagc aaaatcaatg ggaattccaa ttaaatgggg taaaaatttc
     3601 attaaatggg agaatgattt ctaaaaaacc agaagatagg atagggatga aaaaatgaca
     3661 cgaaatattg gtttaccgat taaaaaccca aaaaagaaag cagatgatgg tgataacaaa
     3721 aatccattta atggttcatt atctatacgt ggtaaaatct ttgagggtat agttatcaaa
     3781 tctatggcaa aaaatactgt gatacttgaa aaagaagctc caatttattt tgaaaaattt
     3841 aaaagatatg gtagaagtaa aaatagaata catgcgcatg taccttcaaa tttagatgtc
     3901 aatgatggcg atcgtgttat ggcagctgaa tgtagacctc ttgcaaaatc tgtttcgttt
     3961 gtggtagtgg aggttaaaaa ataatgggtg gtgcacgtag taaagcatca aaaggtgtag
     4021 ttgaatttag gccttatgtc actagagtta ttccagttgg tgcacaaatt gtctgtgcag
     4081 ataatacagg tgcaaaaata ttggaaatag taaatgttca taaatataaa actagagtgt
     4141 caaggctgcc atctgcagct gttggtgatt tctgtaatgt tgttgtaaag aaaggtcctg
     4201 cagaattaag aaaacaggtt tatggtgcag ttattattag acaaaaatat gcaattagaa
     4261 gattgaatgg ggttagagta tcatttgaag ataatgcagc tgttttgatc acgcctgaag
     4321 gtgaagtaaa aggaactgac attaagggac ctgtctctgc agaagcagca gaaaaatggc
     4381 cacgtattgc taacttggca tcagtggtga tatgatatga aaccaggtaa agttagaaat
     4441 cagcaaattt atcgtgcaac taaccaagta cgaaataaac aattaggtgc atcactatca
     4501 aaacaacttc gacagaaata tggacgtcga catatcagaa ttcttgatgg agatgttgta
     4561 aaagtaactc gtggtgaata taagggaatt aatggaaaaa taactaaaat ttcacttgat
     4621 ggaaataggg ttgcgattga aggaatcaaa aaagaaaaaa taaaaggtga taaagttgat
     4681 attttaattc acacttctaa tctacttatc acatcactca aaacagatga taagtggcgc
     4741 acaaaaatac tagaagctaa gactaaacct acagaagcta agactaaacc tacagaagct
     4801 aagactaaac ctacagaagc taagactaaa acaaaatcta gtaataaaaa ggaggatgaa
     4861 aaataatggg
//
LOCUS       DASCPJ010000006         5024 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000061143, whole genome shotgun sequence.
ACCESSION   DASCPJ010000006 DASCPJ010000000
VERSION     DASCPJ010000006.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000061143"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /gene="trpS"
                     /locus_tag="QGF01_00230"
     CDS             complement(<1..848)
                     /gene="trpS"
                     /locus_tag="QGF01_00230"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HJN19365.1"
                     /translation="MADDFVVTPWNVEGDIDYDKLIKRFGTQKITSDLLTKIEKFTKE
                     SHFMLRRGIFFSHRDLNRLLDDYEKGKKFFLYTGRGPSGRTHIGHLVPWVFAKWLQDK
                     FGAKMYFQLTDDEKFWSKKDMTLEKTSNFAFENALDFIALGFKPENTKIIINTKNIKT
                     LYPIAAEFAKRINFSNTKAVFGFQNETNIGMIFYTSLQSAPCFIEDMPVLIPFGVDQD
                     PHFRITRDIAPKINKPKPALIHNIMIPALGGPKGKMSASNENETIYTTDSPEVVRKKI
                     NKYAFSG"
     gene            972..2366
                     /locus_tag="QGF01_00235"
     CDS             972..2366
                     /locus_tag="QGF01_00235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HJN19366.1"
                     /translation="MSHVLHDIEKQIIKLLQSTPNLTEDQLSHETKLSMDQIRRGVEW
                     LRQKSLADVTDTTNLSYSLGKNGSDALKNGLPERRLVNLVKDGPKTFDEIRSNLHGPD
                     FNAAIAHAKKNDWINIEKTDTGSKISLKQEPTESQDEHAISLIDKAQKPRLDLVKSLM
                     ERPDFIIKHEEKTKTVSLSETGKHIDLEKLESGAIDVEADVPHVHAARIHPLKDTINE
                     IRETFVHLGFTEINGNLSQSSFWNFDALFTPQDHPARELQDTFYLRDLNAKQLATPTQ
                     IKNVSNAHKKGWRYYWDIQEARRMVLRTHTTCVTIKHLADKKPDEARIFSLGRVFRNE
                     KLSFKHLAEFNQVEGVVVGKHMTLRDLMGIQKEFYRKIGLTKVKFWPTFFPYTEPSLQ
                     SMVYNEKLGKWIELFGMGIFRPEVTKPLGITKPVLAWGGGIERIAMLKFGVDDVREFY
                     NNNLSWLRTATKCQ"
     gene            2357..4000
                     /gene="pheT"
                     /locus_tag="QGF01_00240"
     CDS             2357..4000
                     /gene="pheT"
                     /locus_tag="QGF01_00240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HJN19367.1"
                     /translation="MPVVELSLSRLEKLIGKNTNKKQILDSLPFLGLDIESLGRNVVR
                     VEYSPNRPDYSTDFGIALGLQGILGISKGMPRLNIRKKGNYEIKVDSSTSRIRPFVTG
                     IIARNGSLNDEAVKQIINMQEDLHFGLGRKRKKSSIGVHDLDRISFPLKYTTTSRDHR
                     FVPLNSTKESSISEILRDSEVGRDYGSILGQSAKVPIITDAKGQTISFPPIINAALTT
                     VTTKTKNILVEVTGIDKQSAEDMLSVVVAILQGAGFQFSQLKVSGSKNSTPNFAPRSI
                     VFDADPVNKILGLTISNSMLVSCLKKCRLDAVRKGKKIQCTIPRYRFDIFGGMDIVEE
                     IALGYGIDNLKPAWPASVHIGEKNTTSEKLDSISRLMTGFGFMETLNSTLTSEQILYE
                     MTNKDSSQIISVISSKSQEHTILRDSILPGLLENLSKNIHETYPQKFFETGIVFSKGS
                     PIKETTNLACVSASEETNFSEMKSVLQSLFRIGFNIKCETKTLSEHALFTQGRVAEII
                     VGKKTVGYIGELDSKVLENFRIRTKAVAFEIKLNGLIFD"
     gene            4207..4623
                     /locus_tag="QGF01_00245"
     CDS             4207..4623
                     /locus_tag="QGF01_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HJN19368.1"
                     /translation="MVNYWLAKQEPSGPRGYNFSTLEKEKTTVWDGVHNNLALKHMRN
                     MKKNDLVMYYHTGDERQIVGIMTITSKPYPNPEEDNERFVVVDVKYKKRLKNTVSLEV
                     MKKEKSFKNWELLRISRLSVMPVPTDIWNTIIKISS"
     gene            4675..4914
                     /locus_tag="QGF01_00250"
     CDS             4675..4914
                     /locus_tag="QGF01_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HJN19369.1"
                     /translation="MATAYVLINCELGSEEEIIKQLKNLEGVIEVHGTFGAYDILAKI
                     ESSTVEMLRETITWKIRKIEKIRSTLTLMGIEGQT"
BASE COUNT         1759 a          838 c          957 g         1470 t
ORIGIN      
        1 ccagaaaatg catatttgtt aatctttttt cttacaacct caggagaatc tgtggtgtat
       61 attgtttcat tttcattcga tgcagacatt tttcctttgg gaccaccaag tgccggtatc
      121 ataatattat gaattaatgc tggttttggt ttattgattt ttggtgcaat gtctcttgta
      181 attctgaaat gtggatcctg gtcaacacca aacggaatca gtacaggcat atcttctata
      241 aagcatggag ctgattgtaa tgaagtgtaa aatatcattc caatattggt ttcattttga
      301 aatccaaaaa ctgcttttgt gttagaaaaa ttaattctct ttgcaaattc tgcggcaatt
      361 ggataaagtg tttttatgtt ttttgtgtta attataattt ttgtattttc tggtttgaaa
      421 cctaatgcta taaaatcaag agcattttca aatgcaaaat tagatgtttt ttctaaagtc
      481 atatcttttt tcgaccaaaa tttttcgtca tccgtaagct ggaagtacat ctttgctcca
      541 aatttgtctt gaagccactt tgcaaaaacc catggaacca aatgacctat atgcgtacga
      601 ccagaaggac ctctacctgt gtagagaaag aatttcttgc ctttttcata atcatctaat
      661 agtctgttca aatctctatg agagaaaaaa atacccctcc ttaacatgaa gtgtgattcc
      721 ttggtaaatt tttcaatttt tgttaaaaga tctgatgtga tcttttgtgt tccaaatctt
      781 ttgattagtt tatcataatc aatgtcaccc tcaacattcc aaggtgttac aacaaaatca
      841 tctgccattc aagtttgact ttgattcagt gttaaaaagt cttattccat tattttgact
      901 actttctaat caaaaaaact gcaacaattg ttccagctaa tttaattaag tgatttttga
      961 acgatgaact attgtcccat gttttgcatg atattgaaaa acaaatcatc aagcttctac
     1021 agtcaactcc aaatttgact gaggatcaac tatctcatga aaccaaatta tcaatggatc
     1081 aaattaggcg tggagtagaa tggcttcgcc agaaaagtct tgcagacgta actgatacta
     1141 ctaacctttc ttacagcctt ggaaaaaatg gctcagatgc tctaaaaaat ggcttgcctg
     1201 aacgaagact tgtaaatctt gttaaagatg gtcccaaaac atttgatgaa attcgaagca
     1261 atttgcatgg acctgacttt aacgcggcaa ttgcacatgc gaaaaagaat gactggatta
     1321 acatagaaaa aacagataca ggatcaaaga tttcattaaa acaagaacct actgagagtc
     1381 aggatgaaca tgcaatctcg cttatcgata aagcccagaa gccaagacta gacttggtta
     1441 agtcactgat ggaaagacct gactttatca ttaagcacga agaaaaaaca aaaacagttt
     1501 ctctttctga aactggtaaa catattgatc ttgaaaaatt agaaagtggt gcaatagatg
     1561 ttgaagctga tgtacctcat gtgcatgccg ccaggataca tccactaaag gacacaataa
     1621 atgaaatcag ggaaaccttt gttcatctag ggttcacaga aattaatgga aacttatcac
     1681 agtcaagttt ctggaatttc gatgcattat ttactccaca agaccatcct gctcgagaac
     1741 tacaagacac gttttatctt agggatctga acgcaaaaca acttgcaaca ccaactcaaa
     1801 ttaagaatgt atcaaatgca cacaaaaaag gttggagata ctattgggac atacaagaag
     1861 caagaagaat ggtcctgcga acacacacca cttgtgttac tataaaacat cttgcagaca
     1921 aaaaaccaga tgaggctagg atattttcat tgggacgagt tttccggaat gaaaaattaa
     1981 gtttcaagca tttagctgaa ttcaatcagg ttgagggtgt ggttgtagga aaacacatga
     2041 cgctacgaga cttgatggga atacaaaagg aattttatcg taagatcggt cttaccaaag
     2101 taaagttttg gcccacattc tttccttaca ctgagccctc tctacaatct atggtttaca
     2161 atgaaaagct tggaaaatgg attgaattat ttggtatggg aatatttaga ccagaagtta
     2221 caaaaccatt gggaattaca aaacccgttt tagcttgggg tggtggtatt gaaaggattg
     2281 ccatgttaaa atttggagta gacgacgtaa gggaatttta taataacaat ctaagttggt
     2341 tgaggactgc tacgaaatgc cagtagttga gctgagtctt agtaggcttg agaaacttat
     2401 aggaaaaaat acaaacaaga agcaaatact tgacagtctg ccatttcttg gtcttgatat
     2461 agaatcattg gggcgaaacg ttgtaagagt tgaatacagt cccaatagac cagactattc
     2521 cactgacttt ggtattgcat taggactaca aggcattttg ggaataagca aaggaatgcc
     2581 acgattaaac ataagaaaga agggaaatta tgaaatcaag gttgatagtt caactagcag
     2641 gattagaccg tttgttactg ggataattgc aagaaatggc tcacttaacg atgaagcagt
     2701 caaacagatt attaacatgc aggaggactt gcattttgga ttaggtagaa agagaaaaaa
     2761 atcatcaatt ggtgtccatg acctagacag aatatcattt ccgctcaagt atacaacaac
     2821 ttctcgagac cataggtttg ttccgttaaa ctctactaaa gagagctcaa tctcagagat
     2881 tctccgtgat tcagaagttg gaagggatta tggatcaata cttggacaat ctgctaaagt
     2941 tccaataatc accgacgcaa aaggacaaac aatatcattt ccacccatca ttaatgccgc
     3001 gttaacaact gtcacaacaa aaaccaaaaa tattctagtc gaagttactg gcatagacaa
     3061 acagtcagct gaggatatgc tgtcagttgt tgttgcaata ctgcaaggag ctggatttca
     3121 attttcacaa ctgaaggttt ctggatcaaa aaattcgaca ccaaactttg caccacgatc
     3181 cattgtattt gacgccgacc ccgtgaataa aattcttgga ttgaccatct ccaattctat
     3241 gcttgtatca tgtctcaaaa aatgcaggct ggatgcagta aggaagggca aaaaaatcca
     3301 gtgtacaatt ccaaggtaca ggtttgatat ctttggtgga atggacattg ttgaggagat
     3361 tgcactgggc tatggaattg ataacctaaa gccagcatgg ccagcatctg tgcacattgg
     3421 tgagaaaaat acaacatctg agaaacttga ctcgattagc agattgatga ctggttttgg
     3481 ttttatggaa acgttaaatt ctacgttgac aagcgagcaa attttgtatg aaatgacaaa
     3541 caaagactca tcacagataa tctctgtcat aagctcaaag agtcaagaac atacaattct
     3601 aagagattca attctaccgg gattattaga gaatttatca aagaacatac atgaaacata
     3661 tcctcaaaaa ttttttgaga cgggaatagt tttttcaaaa ggttctccaa ttaaggaaac
     3721 aacaaatcta gcgtgtgtca gtgcttcaga agaaacaaac ttttcagaaa tgaagtctgt
     3781 cttgcaatca cttttccgaa ttggatttaa tattaaatgt gaaacgaaga ctttgtcgga
     3841 acacgcactt ttcactcaag gtagggttgc agaaattatt gttggtaaga agacagttgg
     3901 atatatcgga gaattagatt ctaaggttct agaaaatttt agaataagaa caaaggctgt
     3961 tgcctttgaa ataaaactaa acggattaat atttgactag tctgtgtatt actgcccaag
     4021 agcatacgca cagacggact atgatgatga tgatcgttat gatcctgatg atttgatagt
     4081 tcacccaggt gcgtaataag tgggcgcccc ggtttcagat ctaaaggagg tatggctaat
     4141 accaatgaat ctttgcgagt caggaccggg gaacatttaa tttcaaaaat aatatcttgt
     4201 taacaaatgg taaattattg gcttgccaag caagaaccca gtggaccaag gggatacaac
     4261 ttttcaacat tggaaaaaga aaaaacaaca gtgtgggatg gtgttcataa taatttagcg
     4321 ctcaagcata tgagaaacat gaaaaaaaat gatctagtaa tgtattatca tacaggtgat
     4381 gaacggcaga tagtaggtat aatgactatt acatcgaaac catatcccaa cccggaagaa
     4441 gacaacgaac ggtttgttgt ggttgatgtc aaatataaaa aacgattaaa aaatactgta
     4501 tcattagaag taatgaagaa agaaaaatct tttaaaaatt gggaattatt acgaatatca
     4561 agattatctg ttatgcctgt tcctacagat atttggaata caataatcaa aatttccagt
     4621 taaaataatg agaataacag tgtcattgat atacatcggt atcgtaataa taccatggca
     4681 acagcatacg tcctcattaa ctgtgaacta ggttcagagg aagaaataat caagcaactg
     4741 aagaatctag aaggtgttat tgaagtgcat ggaacatttg gcgcttacga catcttggca
     4801 aaaattgaat cctcaacagt tgaaatgtta cgtgaaacca taacatggaa aattcgtaaa
     4861 atagaaaaga tcaggtctac ccttacactg atgggtattg aaggccaaac ataactattt
     4921 tttttattaa catgttattg cattttatct ttgtaataaa agacaaagaa ttgggacaac
     4981 gagataagga atatgaatat gtaaaaaaaa tggctcaatt tttt
//
LOCUS       DASCPJ010000007         3805 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000082213, whole genome shotgun sequence.
ACCESSION   DASCPJ010000007 DASCPJ010000000
VERSION     DASCPJ010000007.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000082213"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="QGF01_00255"
     CDS             <1..102
                     /locus_tag="QGF01_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HJN19370.1"
                     /translation="DITQVTIAKSRRTTTGTMMLARNPVTILSATKK"
     gene            114..836
                     /gene="cobI"
                     /locus_tag="QGF01_00260"
     CDS             114..836
                     /gene="cobI"
                     /locus_tag="QGF01_00260"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HJN19371.1"
                     /translation="MAELVCVGCGPGDPELLTVKAVNAIKAADTIMCPASHEDRPSIA
                     LSVISSIIDKSKNQEIVRLIFPMTKDKSVLESTWKKNAKIMAEKVLSGKNVVYITVGD
                     PYLYSTWIYMNREIRTNHPDMKITVIPGIVSIFTFAAKVGVSVAEGSEKVAVIPSCYD
                     LSSVKEIAKNSESMIFLKDGRYFDQVIQVLKDAGFPDNSIFAIGQDLGTDHEIIRKMT
                     LGEVNDDTLTTKYFSILVVKRV"
     gene            829..1602
                     /gene="cobM"
                     /locus_tag="QGF01_00265"
     CDS             829..1602
                     /gene="cobM"
                     /locus_tag="QGF01_00265"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HJN19372.1"
                     /translation="MSKVFFIGCGPGDPDLITVKAKKLLQKADVVVYSGSLIPEQIMK
                     MCKKAKTFDAAKLVREEIFDILRKNAKKGKNVVRLHDGDPTIYGAIREQMDNLHKEKI
                     NSEVIPGVTSFLASAAALGSQLTLPGVTQTMIITRAEKRTKVPKREKISELAKHRATM
                     IFYLSVQLIDNIVKEAVAGGYPKTTPVGVVYRASWPDQMIITGTLQTISKKIKDQKIT
                     RTAIIIIGDVIKPKSYEYSKLYDKSFSHGFRKAKTNSKK"
     gene            complement(1586..2344)
                     /locus_tag="QGF01_00270"
     CDS             complement(1586..2344)
                     /locus_tag="QGF01_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HJN19373.1"
                     /translation="MARIIVFDSGFGSLSIIKPIQQAVKSDIIYFADQKNFPYGQKSI
                     PELTKIITKRLELLEEKFKPDLIVVGSNTPSLLVQIKKKNVIKVLPPIKMASKISVTG
                     NIAILATHATIKSKKLSEFIKKNKLARRINIKKINASKLVQLVESVKFIDDKNLCEKT
                     INNVLSQEFSKYNIDVATLSSTHLPFLLSFFNKQFPNVNFLDPGQEVADKVRTAVKKP
                     SKRNSMKIFTSSKPEKFQKELSKIGIKQKVTFLN"
     gene            2452..3114
                     /locus_tag="QGF01_00275"
     CDS             2452..3114
                     /locus_tag="QGF01_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19374.1"
                     /translation="MTAFNKTWARQETKSMTDKIRDAVKPQGALKPRIQVAVNKLQVQ
                     TSKMDSMLTKLRERDQQLFARIVTAMQNHDTSASRVLSAELAEVRKVSRTLGNARTSL
                     EQVQMRLTTIHDLGDAMVSIGPAMSTMKGLKSSLSRFMPEADSELTSMTQTLNGLMVD
                     SLSGDSFNAMESDVSNEETDAILQEASAVAEQQTDERFPSIPTSAGPESQSQSNPMNF
                     LE"
     gene            complement(3159..>3805)
                     /locus_tag="QGF01_00280"
     CDS             complement(3159..>3805)
                     /locus_tag="QGF01_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HJN19375.1"
                     /translation="AEFYEKTGINMEKSRFRKLGDKEKAFYAEVAFDFEVETAVGWLE
                     LVACNYRADYDLNSHATKSKEKFAVMDGDEKVLPHVFELSMGIDRSMYTILEHSLRED
                     KENERMVLSLKPYLSPIHVGVLSLVKKDGLAEKTDEIYLNIKRKYDVFLDHSGAIGRR
                     YRRLDEVGAPYAITIDHQTLDDDTVTIRMRDSMEQSRIKISELDEKLSKETAFP"
BASE COUNT         1257 a          676 c          666 g         1206 t
ORIGIN      
        1 gatataactc aagttactat tgcaaagagc cgaaggacta caaccggaac catgatgctt
       61 gcacgaaatc cagttacaat tttatcggct acaaaaaaat aggatgattt ggtttggctg
      121 aacttgtttg tgttggttgt gggccgggag atccggaatt acttacagta aaggcagtaa
      181 atgcgatcaa agcggctgat acaataatgt gtcccgcctc acacgaagat cgaccaagta
      241 tcgcactatc agttatttcc tcaattatag ataaatcaaa aaaccaggaa attgttcgcc
      301 taatttttcc tatgaccaaa gacaagagtg tattagagtc aacttggaaa aaaaatgcaa
      361 agataatggc tgaaaaagta ttatctggaa aaaatgttgt atacattaca gtaggtgatc
      421 catatttgta tagtacatgg atctacatga acagagaaat tagaaccaac catccagaca
      481 tgaagatcac tgtaatacct ggaattgttt caatattcac ttttgcagct aaggtagggg
      541 tcagtgtagc tgaaggttca gaaaaagttg cagtaattcc atcttgttat gatttatcct
      601 ctgttaagga aattgcaaaa aattcagaaa gcatgatctt tcttaaggat ggaagatatt
      661 ttgaccaagt gattcaggtt ttaaaggatg caggatttcc tgataattcc atttttgcaa
      721 taggccaaga tcttggaaca gatcacgaga taatcagaaa aatgacttta ggcgaagtta
      781 atgatgatac tcttacaaca aaatatttct ccatcttggt ggtaaaacgt gtctaaggtt
      841 ttcttcatag gttgtgggcc gggagatcca gacctaatta cagtaaaggc aaaaaaactg
      901 cttcaaaagg cagatgtggt agtttattct ggttcactta ttcctgagca aataatgaag
      961 atgtgtaaaa aggcaaaaac tttcgatgca gccaagcttg ttcgtgagga aatttttgat
     1021 attctcagga aaaatgcaaa aaaaggcaaa aatgtagtac ggctccatga tggtgatcca
     1081 actatttacg gggcaattcg tgaacagatg gataatctac ataaagagaa aattaattct
     1141 gaagtaattc ctggcgtcac atcatttctt gcatctgcag ctgcacttgg ttcacaactt
     1201 acactacctg gagttacaca aaccatgatt attacaagag cagaaaaacg tacaaaggtt
     1261 ccaaaacgtg aaaagatttc tgagcttgca aaacatagag ctacaatgat cttttatctt
     1321 agcgtccaac ttattgacaa tattgtaaag gaggcagtgg caggtggata cccaaagact
     1381 acaccagtgg gagttgttta ccgtgcaagt tggcctgatc aaatgatcat cacaggaaca
     1441 ttacaaacaa tttcaaaaaa gattaaggat caaaaaatta cgcggacagc aataatcatt
     1501 atcggtgatg tcataaaacc aaaatcatat gaatattcca aactatatga taagagtttt
     1561 agtcatggat ttagaaaagc aaaaactaat tcaaaaaagt aactttttgt ttaattccaa
     1621 tcttggaaag ctccttctgg aatttttctg gtttagatga tgtaaaaatt ttcatactat
     1681 ttctttttga tggttttttt acagctgttc taactttatc agcaacctct tgtccaggat
     1741 ctagaaagtt gacattagga aattgtttat taaaaaatga caataaaaat ggcaagtggg
     1801 tactagacaa cgtagcaaca tcaatattgt atttagaaaa ttcctgactt agaacattgt
     1861 taatggtttt ctcacacaaa tttttatcat caataaattt tacagattca actaattgta
     1921 ctagttttga tgcgtttatt tttttaatgt taatccgtct tgccagttta ttctttttta
     1981 taaactcaga taatttttta ctctttatgg tagcatgtgt tgcaagtata gcaatatttc
     2041 ctgtcactga aattttagac gccatcttaa ttggaggtaa taccttgatc acattttttt
     2101 tcttgatctg aactagtaat gatggagtat tagatccgac aacaattaga tcaggcttga
     2161 acttttcttc aagtaattcg agtcgttttg ttataatttt tgttagttca ggtattgatt
     2221 tctgtccata aggaaaattt ttctgatcgg cgaaatagat gatatcagac tttactgctt
     2281 gttgtattgg ttttattatt gatagagagc caaatcctga atcaaatact atgattcttg
     2341 ccataaatca ctcatttctt ttatagtttt tactgtttgt aagctaaatt tagcctaatg
     2401 ttgatctaaa cgagctcctt gcattaagag taaaattttc aagaattttc tatgacagct
     2461 tttaacaaaa catgggcacg tcaagaaaca aaaagcatga ccgataaaat tcgtgacgct
     2521 gtgaaaccac agggtgcatt aaaaccacga attcaggtag ccgtgaacaa acttcaagtc
     2581 caaacttcaa aaatggactc catgttgaca aagcttcgcg aaagggacca acaactcttt
     2641 gccagaattg taacagcaat gcagaatcat gatacatctg cgagcagagt tttgtctgca
     2701 gaattagcag aagtacgcaa ggtttcaaga actttaggca acgcaagaac atctctagaa
     2761 caagttcaaa tgcgattgac tacaattcat gaccttggtg atgcaatggt ttcaattgga
     2821 ccagcaatgt caaccatgaa aggtttgaaa tcatcgttat ccagattcat gccagaagcc
     2881 gattctgaat tgacttcgat gacacaaaca ttgaatggat taatggtaga ttcactatcc
     2941 ggtgattcgt ttaacgccat ggaatctgat gtctcaaacg aagagacaga tgcaatctta
     3001 caagaagcat cagcagttgc agagcaacaa acagatgaaa gatttccatc aataccaaca
     3061 tcagcaggac cagagtccca atctcaatct aacccaatga attttcttga gtagatattg
     3121 agactaggtc tattttttta tttttttaat ttgtaacttt aagggaaagc tgtttccttg
     3181 gaaagtttct catctaactc agaaatcttt attctacttt gttccatgga atcacgcatt
     3241 ctaatcgtaa ctgtatcatc atctaatgtt tgatgatcaa ttgtaatggc ataaggtgca
     3301 ccaacttcat ccaatcgtct gtatcgtctt cctattgcgc cagaatgatc caaaaacaca
     3361 tcatattttc gtttaatgtt tagataaatc tcatcagttt tttctgcaag accatctttt
     3421 ttcactaatg ataaaactcc aacatggata ggtgataagt atggtttaag agataacacc
     3481 attctttcgt tttctttatc ctcacgtagg ctatgttcca gtattgtata catactacga
     3541 tctattccca ttgaaagttc aaacacatgt ggtaacacct tttcgtcacc atccattact
     3601 gcaaactttt ctttactttt agtggcatga ctgttcaaat cataatcagc tctataatta
     3661 catgctacaa gctccaacca accaactgca gtttccactt caaagtcaaa tgccacttct
     3721 gcatagaacg ccttctcttt gtctcctagt tttctgaatc tacttttttc catatttatt
     3781 ccagtttttt cataaaattc tgcta
//
LOCUS       DASCPJ010000008         8973 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000087483, whole genome shotgun sequence.
ACCESSION   DASCPJ010000008 DASCPJ010000000
VERSION     DASCPJ010000008.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000087483"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="QGF01_00285"
     CDS             complement(<1..1330)
                     /locus_tag="QGF01_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN19376.1"
                     /translation="MALIQISNQSTKSLGKKSTIRFTQSICPDCNMILDAEVFERNDQ
                     VFMSKICPTHGECEELYFGSYDMYKKFSTYWVDGKGAHAPNVVMQDKCSCPNNCGLCT
                     NHLSHSGLANIIVTNRCDLTCWYCFFYVKKGLEGAYMYEPTQDQVRAMMKTLRAERPI
                     PGNSMQITGGEPMLREDITDLIKIMKEEHVDHIQMNTNGIRHAMDPEAGRDVRMAGCN
                     NLYLSFDGVTARTNPKNHWEIPHALDTCRKTGTTVVFVPTVIKSINDHELGGIIRYGQ
                     KNMDVVHAVNFQPVSLTGRMGKQEREKYRITVPDCIQRIEEQTDGQVTVDDWYPVPSC
                     MPLTNVIEAFSSKPKYELSIHFACGAGTYIFEDADTKKFVPMTRFCDLQGMLELFEDK
                     SEQIRSGANKYFTMLEVVRKLKGFIDKDKQPAGLDLAKMFSTILMKRSFDA"
     gene            1439..2209
                     /locus_tag="QGF01_00290"
     CDS             1439..2209
                     /locus_tag="QGF01_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJN19377.1"
                     /translation="MSVSEQTRKSLEKIGLTGYEIKTFAALLNTGEITASDLSQKCGV
                     PYSKIYEVLGGLEEKGWIGSDNSRPTKYFAKSPNSALQTTKQRREEEFAKNQKVILND
                     LNPIYKKSGTAERPDIWVLTGTMNIATRILEMIETCREEILIAIPKAGEELVKQALPK
                     LRHLHDKGVKTTILTSDKFDKDVIKGLSKLATIKIKKGLFGGGIISDKHNVVILLGPE
                     ISHSNASEIIAICTDHAELSGFAKEYFEYLLKDTEDGK"
     gene            2209..2667
                     /locus_tag="QGF01_00295"
     CDS             2209..2667
                     /locus_tag="QGF01_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HJN19378.1"
                     /translation="MNSADDEILFIGTAEAEHVEMYLKAIWHIKERNEPVKISTIAKM
                     LHVRQPSVVQMLKKLNIKDLVEYNKAGVSLTENGERIGASMMRNSRLLEVLMDSALKV
                     EIDEEMVCGIEHHMNKQFTDALCTMLNHPRKCPHTHDIPMGECCENKDSN"
     gene            2727..2891
                     /locus_tag="QGF01_00300"
     CDS             2727..2891
                     /locus_tag="QGF01_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19379.1"
                     /translation="MTCFCGCEEFEKNPNGQFVGCVKCGHGLQNHDTKYRESARTNEE
                     QSQKRDTDFG"
     gene            3054..3446
                     /locus_tag="QGF01_00305"
     CDS             3054..3446
                     /locus_tag="QGF01_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19380.1"
                     /translation="MVTIDTPASLENFRKFVIASTCSSFAPQSYIEDTEVFAEREQNL
                     GSIYVEAADKVTLKKIRDITFVNARDVLGIIYNSKTGNTSLKWRQHSRNNGRVIGEAS
                     SNSIVNLSSCGVITLDWVENYVKNNKKD"
     gene            3476..4528
                     /locus_tag="QGF01_00310"
     CDS             3476..4528
                     /locus_tag="QGF01_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HJN19381.1"
                     /translation="MLTKTVDENSISCIPEDSDDLVSLRRIIKKGDRVVGETVRVIKQ
                     EKDFARPDKGERVKIKLVLEVEKISLDNVLDRIRVGGIIKESNNESVPHGSHHSFIIK
                     IDQSFNLIKKKWNSIEKKLIRNRDQQSTYILIAIDTGDCGIGKLRGTHLHLLPNMYSG
                     SSGKRYKTTFKIEDFFNEISKAILSIIKNDDQIIIFGPGETRKKFANFLQNMQIGKKH
                     QIKIIEGIDSGGEDGIHLFTKSKSMKEIISTSKLAKVHDIIDQIMFLANKKSRKFTMG
                     LEETMKANEYGAIDSLVFSEGVIQNYDEQKIIDFLNDVESKGTKVYSVDATTDVGLRV
                     TGLGGIISLLRFAVEG"
     gene            complement(4525..4836)
                     /gene="cutA"
                     /locus_tag="QGF01_00315"
     CDS             complement(4525..4836)
                     /gene="cutA"
                     /locus_tag="QGF01_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HJN19382.1"
                     /translation="MQPIIIISTFPSKQSITKISNQIIRKNLAACVNIVKISSVYAWK
                     GKIENQPEYLALFKTTQKNESNLKKELIKLHPYDVPEIAKINVSSMNKPYLNWLIDST
                     N"
     gene            4925..6079
                     /locus_tag="QGF01_00320"
     CDS             4925..6079
                     /locus_tag="QGF01_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJN19383.1"
                     /translation="MAWVVILIIAKSLKLENHGFQIKAYSLTYKNTQVQSALSKMLTR
                     TKRGIRVFADVSVVAGFLMMGFAFWFLLTNISNFFVEPTEFAELTVLIPGVTLTSASA
                     ILYFLLSIPIVLIVHEGAHGIVATLEKIKIKTGGFAIFIAMFAGFVEPDEDDFDKAKK
                     ISRLRVIGAGATANVIFAFALGLLLLTNPFFALILPEPMLGWFYEAPDGVLILSIIPD
                     SGAEKAGLQKNDLITGINGVVIVAPPDFQKVDLNPGDTAIVTILRDGTSLQLPVEIMP
                     SPDDPEKGLVGIMRENALSYKPVYNIIEWDPQVSMFLLWLWMISFFIGIINMLPLPIL
                     DGGKFIQTIIDKKVSEKKANGIMWAIYACTFIIFALNIGLSYMKSGWFTI"
     gene            6089..7033
                     /locus_tag="QGF01_00325"
     CDS             6089..7033
                     /locus_tag="QGF01_00325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HJN19384.1"
                     /translation="MKILFISPTFSGAGGIGPHAFRVSEKLRQEGHDIELMDTPHIPI
                     KNLKNVSFSVFGTLKALSNSKTYDAVHAWNVPSAFIMKHIKAKKKILSIHGIYSDQVK
                     MLHSKLAGSIVTSKEDQVLDWADVLTTDSKYVQTEYKKKLGKDFEYIPAPLDPKKFVD
                     IPDVKKNQKQVVYVGRDSFEKGIDILKEIESKIDGNVKYCTSLPWNKAMEVLKASQVF
                     VLPSRVESIPQSIKEAFFLKIPVVATDVGGVHELVTDNKTGLLVAPNNSKELLDKINY
                     LLNNIELCNILANNAYEFITKNFSWEALLSKYIKLYES"
     gene            7074..8120
                     /locus_tag="QGF01_00330"
     CDS             7074..8120
                     /locus_tag="QGF01_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJN19385.1"
                     /translation="MKLSILDHIVCPTCSSSFKLKIKSQLKNEIKEGTLICKKCNDKF
                     KISKGIPRFVVDQTKDFVRTEMAFSEKWKIHHQNHHAKDWIEFQRKWFLERFDWKSFS
                     MFKEFLKSKNHILDAGTGIGNSAKMLSVNKSTTVFALDASDSIDFAYRNYGSRSNIHF
                     IQADIRQLPFKKSFFDYIFSDQVLHHTKNTATSFKYLTKFLVKSGFISIYVYNKKAPI
                     REYVDDYVRKKTVKMSVAECTEFSKDMAYLGKALSKLKKKIIIPRDIPLLGVKAGTYD
                     VQRFVYWNFLKCFWDESDNFQRSVGVNFDWYYPKFAYRHTASEVKKWFRNAKLRITTL
                     KEIESGISVTGVKR"
     gene            complement(8124..8630)
                     /locus_tag="QGF01_00335"
     CDS             complement(8124..8630)
                     /locus_tag="QGF01_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HJN19386.1"
                     /translation="MIEKYEDPFVKIANMIGGDEYLKVARSLLKAQDATDEEIASSTG
                     LRINMVRKILYDLFGKALITGIRVKDERKGWFVYRWRSRKDEVENFIEKEKKKISDRL
                     QQRLDYENSSQFYHCGNDDCLRVTFEDGLEQFFKCSSCGQVLNLKKNEKSKKAFSKKI
                     DEMKKDMR"
     gene            complement(8656..>8973)
                     /locus_tag="QGF01_00340"
     CDS             complement(8656..>8973)
                     /locus_tag="QGF01_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HJN19387.1"
                     /translation="KKNMATKIVHLTMYGQNINDIQTKLQNEDKLLIVVGAEKVPRTI
                     YDTADYNVAIGNQPHSEISALGILLDRIQQGRQFDLKFKNSERVIIPQEHGKSVQMNK
                     LTD"
BASE COUNT         3122 a         1386 c         1542 g         2923 t
ORIGIN      
        1 ctgcatcaaa cgaccttttc atgagaatag tgctaaacat tttagctaaa tctagacctg
       61 caggctgctt gtccttatca atgaaaccct ttagttttct tacaacttca agcattgtaa
      121 agtatttgtt agcaccagaa cgaatttgtt ctgatttatc ttcaaacaac tcaagcattc
      181 cttgtaggtc acaaaatcgt gtcattggaa caaatttctt agtatctgca tcttcaaaaa
      241 tatacgtacc tgcaccacat gcaaaatgaa ttgatagttc atactttggt ttacttgaaa
      301 atgcctcaat cacatttgta agtggcatac aacttggtac aggataccaa tcatcaacag
      361 ttacctgacc atctgtttgt tcttctattc tctgtataca atcaggcaca gtgattcgat
      421 acttctctct ttcctgtttt cccattctac cagtaagtga tactggttga aagtttacag
      481 catgtacaac atccatgttt ttttgtccat atctaataat accaccaagt tcatgatcat
      541 taattgattt aattacagtt ggaacaaaga ctacagttgt tccagttttt ctacatgtat
      601 ctaaagcgtg tggaatttcc caatggtttt taggattagt tcttgctgtt acaccatcaa
      661 aactaagata aagattgtta catcctgcca ttcttacgtc tctaccagct tctggatcca
      721 ttgcatgtct aattccattt gtattcattt gtatatggtc aacatgttct tctttcatta
      781 ttttgatcaa gtcagtgatg tcttctctta acattggttc accaccagta atctgcatag
      841 agtttccagg aattggtctt tctgctctaa gtgttttcat cattgcacgt acttgatctt
      901 gtgttggttc atacatgtat gcaccttcaa gacccttctt tacataaaag aaacagtacc
      961 aacatgtaag atcacatctg tttgtaacta taatatttgc aagtccacta tgggaaaggt
     1021 gattagtaca aagcccacaa ttgtttggac acgaacattt gtcttgcatt actacatttg
     1081 gtgcatgagc tccttttcca tcaacccaat aagtactaaa tttcttatac atatcatatg
     1141 aaccaaaata gagttcttca cattctccat gtgtaggaca aatctttgac atgaacactt
     1201 gatcatttct ttcaaagact tcagcatcaa gaatcatgtt acagtcaggg caaatacttt
     1261 gagtgaaacg tatagttgat tttttaccca aagatttggt agattggtta gaaatctgaa
     1321 ttaatgccat tattactaaa ttttttaaaa attagtatat aatggctttc ccactctaca
     1381 ccgttggaat aggagagtaa tttatactat gtatcaataa tatactaaat cgataatgat
     1441 gagcgtttct gaacaaacac gaaaatcttt agaaaaaatt ggtctaactg gttatgaaat
     1501 caaaactttt gctgcattat tgaatacagg agaaattact gcatctgact tgagtcaaaa
     1561 atgtggagtt ccatactcga aaatttatga agtgttgggt ggattagaag aaaaaggttg
     1621 gataggttct gataattcaa gacccaccaa atattttgct aaatctccaa actctgcatt
     1681 acaaacaaca aaacaaagga gggaagaaga gtttgctaaa aatcagaaag ttattctaaa
     1741 cgatcttaat ccaatataca aaaaaagtgg aacagcagaa cgaccagata tttgggttct
     1801 aaccggaact atgaatatag caactagaat tcttgagatg attgaaacat gtagagagga
     1861 aatattaatt gcaattccaa aagctggaga agaattggta aaacaggcat tgccaaaatt
     1921 aagacattta cacgacaaag gagttaaaac cacaattctt acctctgata aatttgacaa
     1981 ggatgttatc aaaggattat ccaaacttgc tacaataaag ataaaaaaag gactttttgg
     2041 gggtggcata atttcagata aacataatgt tgtaatatta cttggtccag aaatcagtca
     2101 ctcaaatgcc tctgaaatta tagcaatatg tacagatcac gcagaactat ctggatttgc
     2161 aaaagaatac tttgaatact tgttaaaaga tacagaggat ggaaagtaat gaattcagct
     2221 gacgatgaaa ttctctttat tggtactgct gaggcagaac atgttgaaat gtatctcaaa
     2281 gcaatttggc atatcaaaga aaggaatgag cctgtaaaaa ttagtaccat tgcaaaaatg
     2341 cttcatgtta ggcaacctag tgtagtccaa atgttaaaaa aactaaatat caaagatcta
     2401 gttgagtata acaaagctgg agtttctctt actgaaaatg gcgaaagaat tggtgcaagt
     2461 atgatgagaa atagtagatt gcttgaagtt ttgatggata gtgcgctaaa ggttgagatt
     2521 gatgaagaaa tggtctgtgg aattgagcat catatgaata aacagtttac agatgcattg
     2581 tgtactatgc taaaccatcc acgaaaatgt ccacacactc atgacattcc catgggagaa
     2641 tgttgtgaaa ataaagactc gaactagaga cgattcacat aattataata agatgaaaat
     2701 atagagctat ataggtaaat gaaataatga catgtttttg cggatgtgaa gaatttgaaa
     2761 aaaacccaaa cggtcaattc gttgggtgtg taaaatgtgg tcatggttta cagaatcatg
     2821 atacgaaata cagggaatct gcaaggacaa atgaggaaca atcacaaaaa cgtgatactg
     2881 actttggtta attctttatg attatacaaa aacaataatc gcaggctcag aactcaagat
     2941 ctttatcacc aaatcaaagg gatatgttca tcaatgcctg caaaaagagt tgttatagat
     3001 actaaatgag tttttcaata aacttgataa atagcaatag acatgaaaaa tgaatggtaa
     3061 ctatagatac acctgcttct ttggaaaatt ttcgaaaatt tgtcatcgca agtacatgtt
     3121 catcatttgc tccacaaagc tacatagaag atactgaagt ttttgctgaa cgtgaacaga
     3181 atcttggttc aatatacgtt gaagccgctg acaaggttac tttaaaaaaa attcgagata
     3241 ttacatttgt aaatgcaaga gatgttttag gaataatata taattcaaag actggaaata
     3301 ctagtctaaa atggcgtcag catagcagga ataatgggag ggttattggt gaagcatcat
     3361 caaactctat tgttaattta tcatcttgtg gtgtaattac tctcgattgg gtagaaaact
     3421 atgtcaagaa taacaaaaaa gattaacatt ttttgtttct cattaaaaag ataaaatgct
     3481 aaccaaaact gttgacgaaa actcaatatc atgcatacca gaagattccg atgatctagt
     3541 ctcacttcgt agaattatta aaaaaggaga tagagttgtt ggtgaaacgg tccgtgttat
     3601 caaacaagaa aaagattttg cgagacctga caaaggagaa cgagtgaaga tcaaactagt
     3661 acttgaagtt gaaaaaatat cattagataa tgtactagat cgaattcgag ttggtggaat
     3721 aatcaaggag tctaataatg aatcagttcc tcacggatca catcactcat ttattatcaa
     3781 aattgatcaa tcatttaacc taatcaaaaa gaaatggaat agcatagaaa aaaaactgat
     3841 aagaaacaga gatcagcaat caacctacat cttaattgca atagatactg gtgattgtgg
     3901 tatcggaaaa ttaaggggaa cacatttgca tttactacca aacatgtatt caggatcaag
     3961 tggaaaacgt tacaaaacca catttaaaat tgaagatttc ttcaatgaaa tttcgaaagc
     4021 aatactatct ataataaaaa atgatgatca gattataata tttgggccag gtgaaacaag
     4081 aaagaaattt gcaaactttc tacagaacat gcaaattgga aaaaaacacc agataaaaat
     4141 aattgaaggt attgattctg gaggtgagga tggaattcat ttatttacta aatcaaaatc
     4201 aatgaaggaa attatctcta ccagtaaact tgcaaaagta catgatatca tagatcaaat
     4261 tatgttcctt gcaaacaaaa aaagcagaaa gtttaccatg ggtttagaag aaaccatgaa
     4321 agcaaatgaa tatggtgcga tagactctct tgttttttct gagggagtaa ttcaaaatta
     4381 tgatgagcag aaaattattg attttctaaa tgatgtagaa tcaaaaggca ccaaagtcta
     4441 tagtgtagat gcaactacag atgttggtct aagagtaact ggacttggtg gaattatatc
     4501 tcttctaaga tttgcagtgg aaggttagtt tgttgaatct atgagccagt tgagatatgg
     4561 tttattcatg gaactgacgt taatttttgc aatttctgga acatcgtatg gatgaagttt
     4621 tatgagttct ttttttagat ttgattcatt tttttgcgtg gtcttgaata aggcaagata
     4681 ttctggctga ttttcgattt ttcccttcca cgcataaaca gacgaaatct ttactatatt
     4741 gacacatgct gcaaggtttt ttcttattat ttgattggag atctttgtta tcgattgttt
     4801 actaggaaat gtggatatta tgattatagg ttgcatagta accgataaat ttacgtgaat
     4861 aaaaaaagta ccaatcgatt tggaacttga ttttttaact caaaatgcaa taatctacgt
     4921 tttgatagca tgggttgtta tcttaattat agccaaatct ctaaaactgg aaaatcatgg
     4981 ttttcaaata aaggcttata gtttaaccta caaaaatacc caagtccaat cggctctttc
     5041 aaaaatgctt actagaacta aacgcggaat tcgagttttt gcagatgtta gtgttgttgc
     5101 aggattttta atgatggggt ttgcattctg gtttttactt acaaacatct caaacttttt
     5161 tgtagagcca actgaatttg ctgaattgac agttttgata ccgggtgtta cactaacttc
     5221 tgcctcggcg atactttatt ttttattatc aattccaatt gttttgatcg ttcacgaagg
     5281 tgctcatggt atagtagcaa cacttgaaaa aataaagatc aaaacaggag gatttgctat
     5341 atttattgca atgtttgcag ggtttgtaga acccgatgaa gatgattttg ataaagcaaa
     5401 aaaaatatca aggttaagag ttataggtgc tggagcaacc gcaaatgtaa tctttgcatt
     5461 tgcattggga ttattactgt taaccaatcc atttttcgca ctaatcctac ctgaacctat
     5521 gttaggatgg ttttatgaag cacccgatgg agtcctaata ctttcaatta ttccagatag
     5581 tggtgctgaa aaagctggtt tgcaaaaaaa tgatcttata actggaataa atggtgttgt
     5641 gatagtagct ccgccggatt ttcaaaaggt tgatctaaat cctggggata cagctattgt
     5701 tacaatttta agggatggaa cttcgttgca attacctgtt gagattatgc cttctccaga
     5761 cgatcctgaa aaaggattgg ttggtatcat gcgggaaaat gcgttatcct acaaacctgt
     5821 ctacaacata atagaatggg atcctcaagt gtccatgttt cttctttggc tatggatgat
     5881 ttcatttttt attggaataa taaacatgct tcctcttcct atactggacg gaggtaaatt
     5941 tattcaaacc ataattgata agaaagtttc agagaaaaaa gctaatggaa taatgtgggc
     6001 tatttacgct tgcactttta ttatatttgc tcttaacata ggactgtctt atatgaaatc
     6061 aggttggttc acaatctaat atgatataat gaaaattcta tttatttctc caacattttc
     6121 tggagcaggt ggaataggac cacatgcatt tcgtgtttca gaaaaactga ggcaagaggg
     6181 tcacgatata gagttaatgg atacaccaca cataccaata aaaaacctaa aaaatgtcag
     6241 tttctctgta tttggaactt taaaggcatt atccaattca aaaacttatg atgctgttca
     6301 tgcatggaat gttccatcag cattcataat gaaacacatt aaggcaaaaa agaaaatact
     6361 ttcaattcat ggtatctatt cagatcaagt gaaaatgtta cattccaaat tagcggggag
     6421 tattgttact tctaaagaag atcaggtttt agattgggct gatgttttaa caactgattc
     6481 aaaatatgtt cagacggaat ataagaaaaa attaggaaaa gattttgaat atattcccgc
     6541 gcctttggat ccaaaaaaat tcgtagacat tccagacgtt aaaaaaaatc aaaaacaagt
     6601 tgtttatgtt ggacgtgata gttttgaaaa aggaatagac attctaaaag aaattgaatc
     6661 aaagattgat ggtaatgtga aatactgtac atcacttcca tggaataaag ctatggaggt
     6721 actaaaggca tctcaagttt ttgtgttacc atctagagtt gaaagtattc ctcaatccat
     6781 taaagaagcc tttttcttga aaatccctgt ggtcgctaca gatgtaggtg gtgttcatga
     6841 gcttgtaact gataataaaa ctggcttact ggtagcacca aataattcta aagaattact
     6901 tgataagata aattaccttt taaataatat cgaactttgt aacatacttg ctaataacgc
     6961 atatgagttt attacaaaaa attttagttg ggaagcctta ttgtcgaaat atatcaaact
     7021 ttatgaaagt taatggtatg tcttttatta aataggtata atgtaatttt ataatgaaac
     7081 tttcaatttt agatcatata gtttgtccaa cttgtagttc cagctttaag ctcaaaatta
     7141 aatcacagtt aaaaaatgag attaaagaag gcacattaat ttgtaaaaag tgtaatgata
     7201 aattcaaaat ttcaaaaggc attcctaggt ttgttgttga tcaaacaaaa gatttcgtta
     7261 gaacagaaat ggcattttca gaaaaatgga aaattcatca tcaaaatcat catgcaaaag
     7321 attggatcga atttcaaagg aagtggtttt tggaaagatt tgattggaaa tcatttagta
     7381 tgtttaagga attccttaaa tctaaaaatc atattctaga tgccggaact ggtattggta
     7441 atagcgcaaa gatgttatct gtgaataaat caactaccgt atttgcgctt gatgcaagtg
     7501 atagtattga ttttgcatat agaaactatg gaagtcgttc taacatacat tttatccaag
     7561 ccgacatacg tcaacttcca tttaaaaaat cattttttga ttatattttc tcagatcaag
     7621 ttttacatca taccaaaaat acagcaacat ctttcaaata cctaacaaaa tttttggtaa
     7681 aatctggttt catctcaatt tatgtttata ataagaaagc accaattcgc gaatatgttg
     7741 atgattatgt tagaaaaaaa actgtcaaga tgtctgtggc ggaatgtact gaattttcca
     7801 aagatatggc atatcttgga aaggcattat ctaaattaaa aaagaaaata ataataccac
     7861 gtgatattcc attgttaggt gttaaggctg gtacatatga tgtacaaaga tttgtctatt
     7921 ggaatttttt gaaatgtttc tgggacgagt cagataattt tcaaagaagt gtaggagtaa
     7981 attttgattg gtattatcca aaatttgctt atcgtcatac tgcatcagaa gttaagaaat
     8041 ggtttcgtaa tgcaaaattg agaattacaa cattaaagga aattgaaagt ggaattagtg
     8101 taactggagt aaagcgttaa tcattaacgc atgtcttttt tcatctcatc aattttcttt
     8161 gaaaatgctt tttttgattt ttcatttttc ttcagattta aaacctgtcc acatgaacta
     8221 catttgaaaa attgttctaa cccatcttca aatgtaactc tgaggcaatc atcatttcca
     8281 caatgataaa attgtgagga attttcataa tcaagtcttt gttgaagtct atcagaaatt
     8341 ttcttcttct ccttttcaat aaaattttct acttcatctt ttctagaacg ccaacgatac
     8401 acaaaccaac cttttctttc atcttttact ctaatccctg taatcaatgc tttaccaaaa
     8461 agatcgtaaa gaatctttct gaccatatta attctcaatc cagtagagct tgcaatttct
     8521 tcatctgttg catcctgtgc ttttaggagt gatctagcaa ctttgagata ttcgtcacca
     8581 ccaatcatat ttgcaatttt tacaaatgga tcttcatatt tctcaatcaa taatatcaca
     8641 caatatatcc tagtattaat ccgttagttt attcatctga acagatttgc catgttcttg
     8701 aggaattata actctctcag aatttttaaa ttttaaatca aattgccttc cttgttgaat
     8761 acgatctaat agtataccaa gagcacttat ttcagaatgt ggctggttac ctatagccac
     8821 attataatct gcagtatcat agattgttct aggaactttt tccgctccaa ccacaatcaa
     8881 gagtttatcc tcattttgta gtttagtttg aatgtcattg atattttgac catacatagt
     8941 aagatgaaca atttttgtag ccatattttt ctt
//
LOCUS       DASCPJ010000009         6814 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000133808, whole genome shotgun sequence.
ACCESSION   DASCPJ010000009 DASCPJ010000000
VERSION     DASCPJ010000009.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000133808"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="QGF01_00345"
     CDS             complement(<1..550)
                     /locus_tag="QGF01_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN19388.1"
                     /translation="MLESIIDQSKILERISAGEELTFQDGVDLLEHDNLHVLGMTANS
                     LRQKYVGDQVTFTSSSYVNYTNVCAASCQICAFYRKEGDDDSYTLTPEQIEKRVTTAK
                     SMGATEIHIVGGFHPKLSLDYYESMMKIIKTNHPTLKIKAFTAAEIFFFSKITKNSIK
                     EILSRLKDAGLDSLPGGGAELFD"
     gene            612..752
                     /locus_tag="QGF01_00350"
     CDS             612..752
                     /locus_tag="QGF01_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19389.1"
                     /translation="MVLPLIDENKPKCFVCQTMFENMEELKKHQETIHQEFFQKLADD
                     KE"
     gene            complement(765..1244)
                     /locus_tag="QGF01_00355"
     CDS             complement(765..1244)
                     /locus_tag="QGF01_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19390.1"
                     /translation="MKVTPQNMLDHAHELCEDEDYVNALEYFDIVLEMEPNNLNAIID
                     KGVTFQNLEYFTKALQMYDKALLIEPENSDVLINKGSILHILKKYTEAVSCYDNVIRR
                     DKKNAMALAYKGLSIGEMGNVSLAITYFKKALSIEQNYDLAQSSLLTAKNLLKSHSK"
     gene            1376..1873
                     /locus_tag="QGF01_00360"
     CDS             1376..1873
                     /locus_tag="QGF01_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HJN19391.1"
                     /translation="MTSLLAEYLYAGVFIAALIETIIPPIPTMAVFPTAGFLASQNNF
                     ELYQVVLLGIIGGLGASIGSTVIYLIALKLGRVALLRYLRYVKVSEKKLTRVEKWFQK
                     YGEKAVLFGRMIPVFREMISIPAGLFKMKFAKFLTYTVLGSCGWSITLILVGYYFGNA
                     AFEFW"
     gene            complement(1881..2108)
                     /locus_tag="QGF01_00365"
     CDS             complement(1881..2108)
                     /locus_tag="QGF01_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJN19392.1"
                     /translation="MESVNALITLGKSHLENGSFHEALESFEQAVSLDQNNPDLWNFM
                     GVTLRSLGRYDEAVDCFNKSLKIDPRDKDSS"
     gene            complement(2140..2379)
                     /locus_tag="QGF01_00370"
     CDS             complement(2140..2379)
                     /locus_tag="QGF01_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19393.1"
                     /translation="MKLEKQFDPDFLYNHIVHYYIDKKKCTKEEANKIAQKVINREYQ
                     RRICKNPKCNHMSHDHIRNGDTCFMLDCSCTKFVK"
     gene            complement(2436..3758)
                     /locus_tag="QGF01_00375"
     CDS             complement(2436..3758)
                     /locus_tag="QGF01_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19394.1"
                     /translation="MELLNTPTELRSKILEEAKKIGLTEPCYQKMLDYTIEIFETNGL
                     GTEYYGYHNINHELEVTLGILLIGNNRKNISKLSDDDLKYLFVAALFHDLDPQKYVDK
                     PHEKNVLEHIVIDSTIKDLISKSNLDLEIIKALILRTTYPWTGEAKKECEQEIQEYFK
                     KSKITSDNSEKQEHLMWVGWILSIIDRVIGYALGDFSKAMHLAKTNSHALAWHPSFTV
                     RRSVTYFEDLIGKESEMCEMVLRCLPKYMRKNFMDNVQGFLKLRQQEIQTQSDFLYDD
                     IKFTTKIESTKMKNSEEFINTLHSIYLELPKPLRLERNFKESLTEPTTFLNTLRLRDK
                     NGPIIGFAKGGPLERYQLRSEIKDENFGKNNTIFLEPIALKMGYWGLGGGHQMRQLFV
                     MQAHAMNYEYLTSFALRDVIERRCNGMERAEFVTKFDPERWDYYRIKL"
     gene            complement(3809..3991)
                     /locus_tag="QGF01_00380"
     CDS             complement(3809..3991)
                     /locus_tag="QGF01_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19395.1"
                     /translation="MLLWIVFAAIGILAAIILVYKYSKTSRYDTLGFSINCKTCGKKT
                     NGLKCTYCKEEKHDWR"
     gene            complement(4417..5391)
                     /locus_tag="QGF01_00385"
     CDS             complement(4417..5391)
                     /locus_tag="QGF01_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJN19396.1"
                     /translation="MQEEFEIVIVGAGPAGLSAGIYVARQNVSCLVISKDLGGQMNLI
                     PRLENYPGTIMSSGPILAKTLETQYLSFKGEIIYDTVEKIDESEDGFKIKTNRSEYKS
                     KAIVLAPGKVPNMLGIENESQYYNKGIHYCTKCDAPFYQGKTTASIGVGSYLVESGLL
                     LSRMASKMYLIVKGSKLAGDKDLVAALENNKNIEIVTQSSVKAITGNSVLEQITIVDS
                     SGTEKVLDVDGLFIEMGSKINLDFVKHLVKINTKGEIEIESGGMTSHPAIFAAGDATT
                     IPYKQIVVACGDGSNAGLSAFNYLEKLKGKPGVRADWKKQIGDQVFHY"
     gene            complement(5395..5673)
                     /locus_tag="QGF01_00390"
     CDS             complement(5395..5673)
                     /locus_tag="QGF01_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HJN19397.1"
                     /translation="MTENDSPSNQAYVLLNVDYKHQKDIINKAKTLSTVKNVKTMYGI
                     YEVMIILESNDMHAIKKTIDVDIHNLDGITNMTSLVTVGTKDIHSILE"
     gene            complement(5743..6138)
                     /locus_tag="QGF01_00395"
     CDS             complement(5743..6138)
                     /locus_tag="QGF01_00395"
                     /inference="COORDINATES: protein motif:HMM:NF042555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HJN19398.1"
                     /translation="MEYSESIELYAAPCREIQNEAGVRFAAVIDHSGKKIAGGFRDGV
                     IPFEGDEQKLQDFFKIAVDLSSKNDNFSRSLGALNYIASRRDKVILISFPFPLSEKIL
                     LVSAEPNLEIEKLASKIVNIFSDSSITSD"
     gene            complement(6404..>6814)
                     /locus_tag="QGF01_00400"
     rRNA            complement(6404..>6814)
                     /locus_tag="QGF01_00400"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
BASE COUNT         2172 a         1210 c         1004 g         2428 t
ORIGIN      
        1 gatcaaataa ttcagctcca ccacctggta atgaatcaag accagcatcc tttagtctag
       61 ataatatttc ctttatagaa ttttttgtta tcttagaaaa gaagaaaatt tctgcagcgg
      121 taaatgcttt gatttttaat gttggatgat ttgttttaat gattttcatc atagattcat
      181 agtaatcaag tgagagtttt ggatgaaacc cccctacaat atgtatttct gttgcaccca
      241 tactttttgc cgtagttact cttttttcaa tttgttctgg tgttagtgta taagaatcat
      301 catccccctc ttttctataa aatgcgcata tttgacaact tgcggcacat acatttgtat
      361 aatttacgta tgatgaagaa gtaaatgtca cttgatctcc tacatacttt tgtcgtaaac
      421 tattagcagt cataccaagc acatgaagat tatcatgttc tagtaaatct acaccatctt
      481 gaaaagttaa ttcttcgcca gcagatatac gttctaatat ttttgattgg tctattattg
      541 attcaagcat atatcaataa tagattattt gttaataaaa agataagtaa ggctcatagg
      601 aaattataac cttggtatta cccttaattg atgaaaataa accaaaatgc ttcgtatgcc
      661 agacgatgtt tgaaaatatg gaagaattaa aaaaacacca agaaactatt catcaagaat
      721 ttttccaaaa acttgctgat gataaagaat agatgcagaa taaatcattt tgaatgtgat
      781 ttgagaagat tttttgcagt taaaagactg ctttgtgcaa gatcataatt ttgttctata
      841 gataatgctt ttttaaaata cgtgatagcc aaggaaacgt tacccatttc accaatagaa
      901 agacccttgt aggctagcgc cattgcattt ttcttatcac gcctaattac attatcataa
      961 caagaaactg cttcagtgta ttttttcaat atatgtaata ttgaaccttt attgattaaa
     1021 acatctgaat tttctggttc gattaacaat gctttatcgt acatctgtaa tgctttagtg
     1081 aaatactcta aattttgaaa tgtaacgcct ttatcaataa ttgcattcaa attattaggt
     1141 tccatctcta aaactatatc aaaatattct aaagcattca cataatcttc atcttcacat
     1201 aattcatgag catgatccaa catattttgt ggcgttactt tcataatgat taattatagt
     1261 ggttaataat atattttcaa tttagttttt agttgacaat tacttcagaa actataaaca
     1321 aaccaaacct agtgttatta tgatctgatt ggtttttgag gtatttgttg aatggatgac
     1381 ttcattactg gctgaatatc tttatgccgg tgtatttatt gccgcattaa tcgaaacgat
     1441 aatcccccca attcctacaa tggctgtatt tccaactgca ggattcttgg cttcacaaaa
     1501 taattttgaa ttataccaag ttgttttatt aggtattatt ggtggacttg gagctagtat
     1561 tggttcaaca gtgatatacc taatagcatt gaaactagga cgtgttgcat tattaagata
     1621 tttgagatat gtaaaagtct ctgaaaaaaa attaacaaga gttgaaaagt ggtttcaaaa
     1681 gtatggcgaa aaagcagtat tgtttggaag aatgattcca gttttcagag aaatgatttc
     1741 tattcctgca ggattattca agatgaaatt tgcaaaattc cttacatata ccgttcttgg
     1801 ttcatgtgga tggagtataa ctttaatttt ggttggatat tattttggaa acgcagcatt
     1861 tgaattttgg taaataataa ttaagaactg tctttatcac gtggatcaat ttttaacgat
     1921 ttgttaaaac agtctaccgc ctcatcgtat ctaccaagac ttcgtaatgt aacacccata
     1981 aaattccaca aatcaggatt attttgatca agtgaaaccg cttgttcaaa tgattccaat
     2041 gcttcatgaa aagaaccatt ttcgagatgt gatttaccca aagtgattaa tgcattaact
     2101 gattccattt ttatctcaat aaattattat gcaaaatatt catttcacaa attttgtgca
     2161 ggaacaatca agcataaagc atgtatcacc atttcttatg tgatcatgtg acatatggtt
     2221 acatttagga tttttacaaa tacgacgttg atattcacgg ttgattactt tttgagcaat
     2281 tttatttgcc tcctccttag tacatttttt cttgtcaatg taataatgta ctatatgatt
     2341 ataaagaaaa tctgggtcga attgcttttc taatttcata gtactaatac agcattaaag
     2401 aatataatgc tgatctaaat tattaccaaa atatgctata atttgattct gtaataatcc
     2461 caacgttcag gatcaaattt ggttacaaat tctgctcttt ccataccatt acaacgtctt
     2521 tcaataacat ctcgtaatgc aaaacttgta agatattcat aattcattgc atgtgcctgc
     2581 ataacaaaaa gttgcctcat ctgatgacca ccgccaagac cccaataacc catttttaat
     2641 gcaataggct ctaaaaatat tgtattattt tttccaaaat tttcatcctt gatttcagat
     2701 ctaagttgat agcgttctaa tggtccaccc tttgcgaacc caatgatagg accgttctta
     2761 tctctcaatc gtaatgtgtt caaaaatgtt gtaggctcag ttaatgattc tttaaaattt
     2821 ctttctaatc gtaatggttt tggaagttca agataaatgg aatgtaatgt attgataaat
     2881 tcttcactat ttttcatttt tgttgattca attttagttg taaattttat atcatcatac
     2941 agaaaatcac tttgcgtttg aatttcttgc tgacgaagtt ttagaaaacc ctgaacatta
     3001 tccataaagt tcttacgcat atattttgga agacaacgca gtaccatctc acacatttca
     3061 gattctttcc caattagatc ttcaaaatat gtcacagagc gtctaacagt aaacgatgga
     3121 tgccaggcaa gtgcatgtga atttgttttt gctagatgca ttgcctttga aaagtcgcct
     3181 aaagcatacc caataaccct atcaatgatt gaaagtatcc aacctaccca cataagatgc
     3241 tcttgttttt ctgagttatc actagtaatt ttagattttt tgaagtattc ctgaatttct
     3301 tgttcgcatt ccttctttgc ttctcctgtc caaggatatg ttgttctaag aattaatgct
     3361 ttaattattt caagatcaag atttgattta ctgattagat cttttattgt agaatctatt
     3421 acaatatgtt caagaacatt tttttcatgt ggtttatcta catatttctg aggatctaaa
     3481 tcatgaaaaa gtgcagcgac aaaaagatat tttaaatcat catctgacag tttagagata
     3541 ttttttctat tattaccaat taataatatg cctaatgtaa cttctaattc atgattaata
     3601 ttatgataac cataatactc ggttcctaat ccatttgttt caaaaatttc aatcgtgtaa
     3661 tcaagcattt tttgatagca tggttctgtc agtccaattt ttttggcctc ttctaaaatt
     3721 ttactcctga gctcagtagg agtattcagt aattccatta aaatgtgagc gcatatcttg
     3781 tttttaagtt aataaatctg ggttacgact atctccagtc atgtttctct tctttacaat
     3841 atgtacattt caaaccattt gtttttttcc cacaagtctt acagtttatt gaaaatccaa
     3901 gtgtgtcata tcttgatgtt tttgaatatt tatacaccaa aataattgcg gctaaaatac
     3961 caattgcagc aaaaactatc cataataaca taatatcact tagatatact aagattaaat
     4021 cttccgtaaa aaaaatattg ggtttaaggg tattcataaa attctttata attgaacatt
     4081 ttagtattat tgctgatgat gaagacgatt tgctctgatt attgatgaga tccgcgacga
     4141 tatgaagcat agttaattat tgaattaaca tttttatttt gataaattaa acaaatcatg
     4201 tcgactccaa aaaccgactg aaaatgaggc gttaaaacct cattttcatt tttgatttcc
     4261 actaaattga attatacaga agtaatattg caccaacggt tatgattgca atgggtatta
     4321 atcggcttct tacattcatt ctaaatccta aaaattagta gtgttttcat agtattaaca
     4381 tatttgagat aaatctaatc tattcaataa aaataatcaa taatgaaata cttgatcacc
     4441 tatttgtttt ttccagtcag cacgtacacc tggtttacct tttagttttt caagataatt
     4501 gaatgcagaa agaccagcat tagacccatc tccgcacgct acaacaatct gtttatatgg
     4561 aattgttgtt gcatcaccag ctgcaaatat tgcaggatga cttgtcatac cacctgattc
     4621 tatttcaatt tcacctttgg tatttatttt taccaaatgc ttaacaaaat caagattaat
     4681 ttttgaaccc atctctataa ataaaccatc aacatctaat actttttcag taccggatga
     4741 atcaactatt gtaatttgct ctaaaacgga atttccagta attgctttta ctgacgactg
     4801 tgtaacaatt tcaatatttt tattattttc caaggctgca acaagatctt tatcacctgc
     4861 aagttttgaa cccttcacaa ttaggtacat cttagaagcc attctagata ataataatcc
     4921 agactcaaca agataagatc ctacacctat agatgctgta gttttaccct gataaaatgg
     4981 tgcatcacat ttagtacaat aatgaattcc tttgttataa tactgtgatt cgttttcaat
     5041 acctaacata tttggcacct taccaggtgc taacacaatt gcctttgact tgtactcaga
     5101 acgattagtt ttaattttaa atccatcttc tgactcatca attttttcaa cagtgtcata
     5161 aattatttca cccttgaatg acagatattg agtttcaagt gttttagcca atattggtcc
     5221 acttgacatt atagtgccag gataattttc taatcttgga atcaggttca tttgaccccc
     5281 caaatctttt gatataacca aacatgatac attttgtctt gcaacatata ttccagctga
     5341 aagacctgcc ggtccggctc ctactattac gatttcaaat tcttcttgca tttcttattc
     5401 taaaattgaa tgaatatctt tggtgccaac agtgactaat gaagtcatat ttgtaattcc
     5461 gtcaagatta tgtatatcaa catcaattgt ttttttaata gcatgcatat cattagattc
     5521 taagattatc atgacttcat aaattccata cattgtcttg acatttttta cagttgatag
     5581 tgttttagct ttattgataa tatccttctg atgtttataa tcaacattca ataaaacata
     5641 tgcttggtta ctgggagaat cattttcagt catacaatta tacaaaaata tcttgataaa
     5701 aaagtttcca gggttatcac agtcgaaaaa cgagcaatat tatcaatctg atgttataga
     5761 ggaatcagag aaaatattaa ctatttttga tgccaatttt tcaatttcca ggttaggttc
     5821 tgcagaaacc aacagaattt tttcgctcaa tggaaacgga aagctgatca atatgacttt
     5881 gtctcttctt gacgctatgt aatttaatgc acctaaggaa cgtgaaaaat tatcgttctt
     5941 tgatgaaaga tctactgcaa tcttgaagaa atcttgtagt ttctgttcat caccctcaaa
     6001 aggaataaca ccgtccctaa aaccaccagc aatttttttg ccactatgat ctattactgc
     6061 agcaaatctt acaccagcct cattttggat ttcacggcat ggtgcagcat aaagctcaat
     6121 actttcagaa tattccatat atgacattat aattctgaga atatcaactt atggaaattg
     6181 tgaggacaga atggtattta ccgttaggat tggagttact cttccttgga ctttaggtga
     6241 aatacccctc acaaagtttt cctacagtat accacatata atctaagttc ttcattcatt
     6301 ataccacaaa ttatcttatg aaaatgtatg gtgaatttgt ctatagttta cactattcaa
     6361 attaaaaata tgaatcgtca tgtatagtct ctatgtacgt ggccgtgctg atgtgttggt
     6421 ctattagtaa tggcatgctc accactcgcc gaagcttgtg atctacacat ccattctatc
     6481 aacgcagtct tcttctgcga accttcatct tacgaaaggc tgtcttgtct cgggatgggc
     6541 ttcgtgctta gatgctttca gcacttaacc caaacggctt agctgcccgg cctgccttat
     6601 cagacaaccg gtagaccagt ggccacgctg ctctgttcct ctcgtactag gagcaacttc
     6661 ccctcagaca gccatgcttc catcaggcag agaccgacct gtctcacgac ggtctaaacc
     6721 cagctcatgt tcccttttaa taggcgagca gcctcaccct tggcccctgc tgcaggacca
     6781 ggataggaaa agccgacatc gaggtaccaa accg
//
LOCUS       DASCPJ010000010        26777 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000148252, whole genome shotgun sequence.
ACCESSION   DASCPJ010000010 DASCPJ010000000
VERSION     DASCPJ010000010.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 26777)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26777)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26777
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000148252"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(71..144)
                     /locus_tag="QGF01_00405"
     tRNA            complement(71..144)
                     /locus_tag="QGF01_00405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Gly,seq:ccc)
     gene            365..1516
                     /locus_tag="QGF01_00410"
     CDS             365..1516
                     /locus_tag="QGF01_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJN19399.1"
                     /translation="MIYDVIYEHLKNIRQTKVGTIQSAATIIDPKHTVSQTIAKIIKN
                     DSYDAFSKHGKIVYGTNVRELLSARAVNSMKVEPFLHPIPSVTPNDNLEKVAKIINHH
                     RTRSIPIVEKDEILGVVKAENVLKVLSNLDNKWIKASLIFTPTPITIDHNTLLSSARK
                     IMISKRIDHLPVTDNGQLKQILTSYHVLQTLIPEERLGKKTMAEKRITRFGSKVKNIG
                     TNKISQCKPNDNISTVIKSMLENNTSFSLVMLWDNIQGIITYRDILDLFETKVENDIP
                     IYIIGMPDGERDADMIGSKFKKTLQRLNKTSPEIQEARISIKRQRIWGTKKLYQITAL
                     IITPHKTLSFKEEEWDLSKSIENINQKILKSLSKKDRKRSKTSIRKILR"
     gene            2028..2300
                     /locus_tag="QGF01_00415"
     CDS             2028..2300
                     /locus_tag="QGF01_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19400.1"
                     /translation="MNWYDPDHQSNGATLGDNIGHQENDDWDCEIELSCHKKENQGRV
                     STIFMRYLRDKYGHTADRALKRAQKREQRERHTSTEPDSQIISGVF"
     gene            complement(2297..2506)
                     /locus_tag="QGF01_00420"
     CDS             complement(2297..2506)
                     /locus_tag="QGF01_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19401.1"
                     /translation="MKMRGVFWILIGVVLVPPLSTLWLAPLGVLPHIAFHSPYLLGSL
                     AIGIMFIIKGSLEIRKEKRENPRKF"
     gene            2576..3679
                     /locus_tag="QGF01_00425"
     CDS             2576..3679
                     /locus_tag="QGF01_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJN19402.1"
                     /translation="MIQGFATPEGTASFASKAIAHKENFRKIQNLTLSNIGIGTYLGN
                     QDLETDKQQKTAIKQSILQGVNVIDTAINYRAQKSERTIGKAVSELIEEEKINRDQIF
                     VSTKNGYVTNDGDIPEDFMQYIMREFGKKGIVKEGDISSQYNCITLPFLEDQLARSQK
                     NLGLDCIDLMYLHNAVEGQPQMPHDEFIHHLSDIFEFFEQQREKQNIKFYGLATWECF
                     RVSNDKPNYLSLSEVIELAKKIGGDNHGFRFVQLPYNLSFDQAFMQKNQLADSNNQIT
                     FLEAAIHHNIGVFTSVPLMQGKLLEWIKNNEKFTNQSPSVDALQFIRSTPGVLAPLIG
                     QKQESHVSENLQVLKIPPYTEQEFYDLFKELTG"
     gene            complement(3681..3812)
                     /locus_tag="QGF01_00430"
     CDS             complement(3681..3812)
                     /locus_tag="QGF01_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19403.1"
                     /translation="MKARFNGKCVECNESIKKGKEIVKNSKGVWVHKHCSDVEEELP"
     gene            complement(4255..5466)
                     /gene="coaBC"
                     /locus_tag="QGF01_00435"
     CDS             complement(4255..5466)
                     /gene="coaBC"
                     /locus_tag="QGF01_00435"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HJN19404.1"
                     /translation="MTNHPSLDIVESYGTELSGKKIVLCVAGSVAAYKSIELARLLMR
                     HGANVKCVMSNASTKLIKPDYMKWATGNNVITKLTGNMEHIDLADYKRSDLIIVYPST
                     ANTLGKLANGIDDTPISTVLTVAFGSKIPILMGLAMHASMYENAAVRKNIQFLRRKVD
                     FISPQMIEGKAKAPEPEDVLDFVLTKFGQSKKLKGKKVLMTAGPTVEKIDPVRVMTNI
                     STGKTGTLLASELVSAGAKVTLVYGPGIAEPPKGAKIIRVTTVSEMFSTVKKELRKKF
                     DVVILAAAVSDFIPKNSNSKIKSNRNTTIKLTRAPKIIDHIKKIHKGILIGFKAEANI
                     SKKELVSRTRKKLRESDADLMVANDIGSGYNNSDYNEVFLIDSKNVVKTGRKTKAKIV
                     KIIRKNIEKNL"
     gene            complement(5463..6245)
                     /gene="panB"
                     /locus_tag="QGF01_00440"
     CDS             complement(5463..6245)
                     /gene="panB"
                     /locus_tag="QGF01_00440"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HJN19405.1"
                     /translation="MPKTIDEIVRKKNGQKITVVTSYDYTMATLCDQVDILLVGDSAG
                     MVMLGYENTAPVTIDQMCMFTEGVSNGRKNALIVADLPNKSYENETDAIANSQRLIKA
                     GADAVKLEGRLPEIIKAIVQADIPVMGHLGLLPQTATKYSVQGKTKSDVESLLDDAKA
                     LQEAGVFSIVLEMVTSEATKKVTKMLSVPTIGIGCGQYCDGQVLVIHDMLGLFEKLKP
                     KFAKRYLSLSEEIKKSISQYVKDVESGTFPSDEHSFHMEIQS"
     gene            complement(6238..6972)
                     /locus_tag="QGF01_00445"
     CDS             complement(6238..6972)
                     /locus_tag="QGF01_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate/pantothenate synthetase"
                     /protein_id="HJN19406.1"
                     /translation="MNIPKSHPRFVSLQTREKLVKAFGSGLVAKEGLIAQGRGEALDY
                     LIGEKTSKSAKKAISAAAYTLQNAKNPVISVNGNFAALCTPEIIKLSRILDAKIEVNL
                     FYGPESRKKAIARLLKKSGAKEILGTDLKSARKLKGIDSARRIVDKDGIFSADVVLVP
                     LEDGDRTIALKKAKKKVITFDLNPLSRTAQASDVTIVDNVTRAMKLLVKACKKKKVKP
                     RYNNKEVLAESISEIKKNLKRRARLA"
     gene            complement(6993..7886)
                     /locus_tag="QGF01_00450"
     CDS             complement(6993..7886)
                     /locus_tag="QGF01_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HJN19407.1"
                     /translation="MRAKAFSPAHITGFFKAELDKKEPNQLGSLGAGFSIQKGVKTTV
                     NVRSKKEHDINNYTIRVKGFNTGDIRVSEYVLNEFLLDGKYFDVTHEIDVPVGYGFGC
                     SGAVALSLAIALNDALKYGYTKTKVAQIAHKAEIKCQTGLGDVLASYHGGFEIRTKSG
                     APGVGEVQRITPKEKLEVMIICFNPISTKKFLKEKISLINGLGGKMVQKLVKSQDMDE
                     FQDMSIKFANYIHVITPKMNKVIKDLHDNGIKCGIALFGETIFSLVPKDKKQKALEIL
                     KKYDDGVIITSRIDNSGARLE"
     gene            7950..8939
                     /locus_tag="QGF01_00455"
     CDS             7950..8939
                     /locus_tag="QGF01_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HJN19408.1"
                     /translation="MTTFTRRLQRIGSSILVSLPKEWVVANNLDKSSQVEIETTQNNL
                     SITTQQSKKPTKEVEIPYPLSKEDSVVANITGAYLLGYDLIRIKGKSTISIKDRENIR
                     ASMRSLVGIEIIDEDAKTITTQILLDESAVNPQKILKRMSSIAHGMFIDVLDMLQNPD
                     QLASVVDRDSEINRQYFLLVRLIRSAIVDKRLAATFNLVDMDILDYRLAANIIENAGD
                     TIVSITDTIAKSMAFKTMSKSHFNLISDIAKDIEEIQTKSVDAFVSNDRILAITTIQH
                     HKKLLKKMYGARIPKGTALKETEILPFLDLLQGIENYAKSWSDILDLIQPSYK"
     gene            complement(8940..9452)
                     /locus_tag="QGF01_00460"
     CDS             complement(8940..9452)
                     /locus_tag="QGF01_00460"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HJN19409.1"
                     /translation="MKKKTVYKGKPVSIDVYNLTIEKRKVRREIIQHPGAAAILAFDE
                     KGKVILVKQHRFPHGYILEIPAGTLEKGETPRKCALREIEEETGYRAKKMTHFLTYYP
                     SVGYNQEAIHCFVASGLTKSKTHLDTDEFITVKKMDLKKLINMIKKDKIIDSKTICSV
                     MVYAAQKKLF"
     gene            complement(9454..10371)
                     /locus_tag="QGF01_00465"
     CDS             complement(9454..10371)
                     /locus_tag="QGF01_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HJN19410.1"
                     /translation="MKLSKIDNLDMRILSNLLNNCRESDRQIGQKIGLSGVAVKSRIN
                     KMLKSKLIEKFTLKIEPHLLGYNVIYLVTTGQDVNEIMKQVKLVGEPFFVVPCVGGYS
                     ACGIVVNGEVEQKIAIIKNLLNQVRILNIFEAEDAGIESNLTKTDLDVIEQLLKNPRE
                     QIDVVAKNAKISSKTVTRSIEKLQENPAFQFTLIYNPAKIKPYISHAVLCVVNGNIKN
                     LLKTLEKQFEDHFMQIPFIAKNQIALFLYSEDIFEMDEMVQKASSVENVVAVENFMPK
                     KISLPHDWIRNAIKENRKSERLHLLRITT"
     gene            10475..11755
                     /locus_tag="QGF01_00470"
     CDS             10475..11755
                     /locus_tag="QGF01_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HJN19411.1"
                     /translation="MDYRLNLAKRMLFNKRGSLIGAVLAVSIGILVINVNFVIFQGIY
                     DAIIRDMSDYRFGDIYVYDDENSTIEKSDIVFINWFERMPNVEAATPRLEGSATAETR
                     ILGQAHEKFNVPVLGVDPISDIRTSTIHETVGEGQYVYSRNSIVVGESVASDLGGCSQ
                     DGFFMSGGTDPTVTGSEAISKCQPVRVGDSVKLIITDQWGIDQKKRFVVTGISGTAGG
                     QGFDRSVIIHIDTLRDMLDRSGESRSIMVRLQESTTAYEAAQIKNQFLAAFPNDNLKA
                     ETIEESAESTLSGFRSGIAMINLIGYFGMGSSAFAILTIQMMLVNSKTREIGIMRAIG
                     ARRKDILIIFIMQGMIIGAMGAGAGTALGLGYTTYAKITNMEFQGSLALEVNYNWQKI
                     GETAFLAFILAMIASIYPSYRATKKQPVEAMRTV"
     gene            11778..12455
                     /locus_tag="QGF01_00475"
     CDS             11778..12455
                     /locus_tag="QGF01_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJN19412.1"
                     /translation="MTKIYGEAETKVMALDDVSFSVKKGEFVLIVGSSGSGKSTLLNM
                     IGLLDKPTKGKVSIDGFATSNLTDNEISAFRNKKLGFVFQFANLLADLTVLENVLLPS
                     QIPLDSSIPFANAKKILEKVGLEDQLYKRANKISGGQAQRAAIARGLVNNPTIVLADE
                     PTGNLDSVTAQNIVQLMKKMAKELNQTFIVVTHDRKQFGDVDRIITVKDGHVFEGEHM
                     EQMEVTI"
     gene            12452..13783
                     /locus_tag="QGF01_00480"
     CDS             12452..13783
                     /locus_tag="QGF01_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19413.1"
                     /translation="MNYKLFFLVTIFLSGTCVSSIDAEIQTPPQGVVTDSKRIMGVKF
                     LDAYFGTETTKIEVAPGDKNVPLTVTLANIGTNDIVGIKGQLYLPMGFSSPNGAYKPI
                     YADTNQKALAGANFQLTFFVNISLQADIKTYPATIELDYSRLRESGLHHDAFNFDFKL
                     TGESVINLKAVTPFITSITNNNVVLEISNPGSSSLSNIDIVLQNTSTTTSSTTTSTTN
                     VEKVIFDQNQWKIGNIGPNSSKIFSFNVFVPESLKNEPLRIPLGITYSDAHGEQKSVT
                     RVADLYINGLVNPSIYGVKVIELSGKKTIIGEILNEGNSDGLFGFVKLQPRGDSNIKE
                     SSQYIDEIEPDSPVPFNIPIETNGILSFGEHDIRILVSYKDSVRDEHILTYDTTITIK
                     PFEDNTDYGSMIGGLIFLAFVIAVAYKLYSKNKIPFIKKGQIPFIHKKIDS"
     gene            13834..14217
                     /locus_tag="QGF01_00485"
     CDS             13834..14217
                     /locus_tag="QGF01_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19414.1"
                     /translation="MKEIEVPKNLRQFMIEGAEETNLGDKKGARKQFRYGNLHIREYD
                     DRFTVHSDKIDPRKNPLGHLLMDAPEVLIGLAGAAIGGLAIGAYIYKMKKDSPVKTPQ
                     SIISGLAASLATGYASYIISKKLKE"
     gene            14222..15772
                     /locus_tag="QGF01_00490"
     CDS             14222..15772
                     /locus_tag="QGF01_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HJN19415.1"
                     /translation="MVLVRTIHVFIKLVPVILALRKDRILWIAQEGKGIDEKRFRRNA
                     QRILNTCISLGPVFIKFGQWLSSRADILPQPYLEELSKLQDSVPAAPFDKVRPIIEKD
                     IGKIEEKFDDLDQNSLSGASLGQVHRATKNGQQVIVKIKRPGIEKQVEKDLKVLMKII
                     PFAMKFVDPNLRFSIIPIMKQFADSIHEEMDYTKELDNLKKIKKNMKPYNNVVIPEVH
                     DDYSTKNILTMEYIPGIKITNIEALDKKGIDRQQLVIDVHKVFFTMLLRHSIFHADPH
                     PGNISVKDDGSLILYDFGMIGRLNNETRLRLVRLYLALVEKDPPRTVNAMDDLGMLAP
                     DFNREVIEKGIDMSIKSMYGKKPDEMEVEALMSLANRTMSKFPFKLPKNLALYLRMST
                     IIEGIYHTHKVDFKFIKVLRQILEEESLIKDAYIEEIKHSFKRFAKTLDDTLTIAPEI
                     KKFMDENRVLMQKNKRGSNTLLSGSILSGAVFFGSAFLFQSSETIGTIGIIASAAIMG
                     ICVAARNR"
     gene            complement(15769..16155)
                     /gene="hsp14"
                     /locus_tag="QGF01_00495"
     CDS             complement(15769..16155)
                     /gene="hsp14"
                     /locus_tag="QGF01_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp14"
                     /protein_id="HJN19416.1"
                     /translation="MDLVKNIAKDFFKEIDDKSREFFEFVLPPIDLHEENDTLIVTVD
                     IPGFGKNDINVTLNSNVLSIKAEKKNDVNGRIIMKQRPRIIEKKMRLPITVKEGEEKV
                     NSAKYVNGVLTIKIPITKKGKEISLD"
     gene            16226..16807
                     /gene="tmk"
                     /locus_tag="QGF01_00500"
     CDS             16226..16807
                     /gene="tmk"
                     /locus_tag="QGF01_00500"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HJN19417.1"
                     /translation="MIIAIEGSDQAGKKTQTAMLAKALKSQKIKTAIFDFPDYSTVIG
                     KEIDAYLHGKRKFPPEIIHYLYAVNRWEKVEEIKKSATKNSILIMNRYYHSNIVYGIA
                     NGLKEKWLESLDASVPKSDLVIVLDVSQTESFSRKKSKRDKFEKNKLFLKKISQIYRK
                     LAKKHKWKIIDASGTKQETHKEILKIIFKKIGS"
     gene            16804..18045
                     /locus_tag="QGF01_00505"
     CDS             16804..18045
                     /locus_tag="QGF01_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJN19418.1"
                     /translation="MKKTYLDIIDPIHDFVRVNNSELEIIDTPIFQRLRRIKQLSGAH
                     LIYPGAQHTRFEHSLGVMHITSMACQSLLEKSIIPLDEIQNLRFAGLLHDIGHGPFSH
                     LFEEVLQKNRKISHEDLGRNIIQKTEIGDLISKNGFNKKSIANLAFSNSKHQFMNEII
                     SGALSADMMDYLLRDGYFTGAEHAKIDHKRLTYSLDVYKNKLALDKSALVNFETMLIS
                     RYQMFKAVYFHKTVRAGEVMLLKAMSLAEDELGFSSLDLDEFLKLSDESVLSKLLNLP
                     ESNSDLKTSKKIATNYLNRNLFKCVFERMVTSSQGFSNKMIGELENKIAKKSKIDSNE
                     IHLDTSTTNSIPMTPSKKESKSVILITRKNKIIAEEFPISKIPLVSTISGKMNILRVY
                     TQQNNRKKVEIAAKSILSEIE"
     gene            18045..18725
                     /gene="pyrH"
                     /locus_tag="QGF01_00510"
     CDS             18045..18725
                     /gene="pyrH"
                     /locus_tag="QGF01_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HJN19419.1"
                     /translation="MKKKLVIKLSGKVFAIEETKKLKDYATFFVKKSKICQPIIIAGG
                     GKIARYFINHARSSGADESTLDELGIEISRLNARLLIYALKGKAYPHPPTNLTEVKLA
                     VDSGLIVVTGGLHPGQSTNATAALIAEKVHATEFLNATDVDGIYDSDPNKNKNAKKFK
                     RIELKNLRNLLVHEDSVAGGYDLMDIVALKVIERSKIKTRIIKSDIKTIEKAMKGLST
                     GTEIILPN"
     gene            complement(18726..19079)
                     /locus_tag="QGF01_00515"
     CDS             complement(18726..19079)
                     /locus_tag="QGF01_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19420.1"
                     /translation="MQYFQAVQIGKRVANKAQMALFEITGFAMLTLTTKKIDGKFFPV
                     GEESFAAVIKTEDGFVIILVDEGGFTKAQTKPLDEGEAKSVFQKVLDSGIAEFSDNKI
                     KIWNDVYPTVQNELK"
     gene            19151..19699
                     /locus_tag="QGF01_00520"
     CDS             19151..19699
                     /locus_tag="QGF01_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19421.1"
                     /translation="MNPRIFVGVAVVALVAVLFLFGFSSVISDVSEDGFFTSPSDLGQ
                     TVLPLEIDLHDFSILEVSEKQATLHVEFKVTNPNFKSVMLQLIKYSIYHDDELIEVGE
                     IGTRPEGGITSPNYFIILNERPLPIGEKFTIKNTGNTPELWQALNNDELNWRVTAEIF
                     YNLSSLTSGQENTSTFDFSKQD"
     gene            complement(19999..20685)
                     /locus_tag="QGF01_00525"
     CDS             complement(19999..20685)
                     /locus_tag="QGF01_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19422.1"
                     /translation="MTPKFAGGGAGDKDYKQIVKNALDGIRQDIEIDIDPKDVNTILL
                     HEVTKCLRKSYYDRVDPINVEESNLNQVLGGVFRQMKSKATTNNYKMKDGVELKGQAD
                     MIESDVIMIFRSVEEFPESPLANDILYLNACMWIFDKIEGIITYITRGGKEDSFVVNR
                     NQKMFEEVVRRAMVLHDLLKRQNSDAKAKPPIIEPSVDCNTCQYYSRCYIKKKEGKPI
                     TFRNLFGSKD"
     gene            complement(20729..21331)
                     /locus_tag="QGF01_00530"
     CDS             complement(20729..21331)
                     /locus_tag="QGF01_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19423.1"
                     /translation="MTGEAKRQRYIISHLASEGDSITRTRTAIAQHIAEKNGIVWKNI
                     YSGVFRDLDEVLIPLNIVIEDGRLPLTRGPKALQESGVPFYKLTIKGLLVALGLVELK
                     DKDGVLQQFLSKSEIKENHFKESIKILAKISPSFAYSIFEKYIRAYCDGKVKDIIPFN
                     IIEMKKISGQTFKIQKELLEGFTTLSKSDKNAVLNMFIGK"
     gene            21540..23120
                     /locus_tag="QGF01_00535"
     CDS             21540..23120
                     /locus_tag="QGF01_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HJN19424.1"
                     /translation="MNSKNHKYALLLMAAVAITATGAMSQAYAQQITDGMDGYVVGDP
                     DGGAGIYTGNPNECWHDTDDDGVPDMYCLIDTGDTAWMITASSLVLFMTPGVAFFYGG
                     LARSKNAVNTVGMVFIVMGLMSVQWVLWGYSLAFGPHDNEANMFMGNLDYMGFNQVSA
                     WAPLGEPGPCEGTWSDYYQMQQMKSEDAGLCSQGWPGTVPHQLFAMFQATFAIITPAL
                     IVGGLIDRMKFSALCIFVLLWGTFVYDPIAHWVWGGGYIGNMDFDPDLSPSYGLDFAG
                     GTVVHISSGFAALAGALVLGRRLGYGKVPMEPHNVPMVVLGASILWFGWFGFNAGSEV
                     LSDGIAVSAWTVTNTATGMASVTWLLMSWAHTGKASITGAATGAVAGLVAITPASGWV
                     GPMAAIIIGVAAGTVCYGCVAFKNARKWDDALDVWGVHGMGGFTGAVLTGTLASPHVW
                     DTGDGIGAWTGTPEGYEQQAINIAGALLSVAYAFGITIVILKIMDAVWPGGIRVTPKE
                     EEIGLDLAQNGERAYVNE"
     gene            23202..23978
                     /locus_tag="QGF01_00540"
     CDS             23202..23978
                     /locus_tag="QGF01_00540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HJN19425.1"
                     /translation="MFKTTSELEKILDNPNLLLIDTRSFQEYSNGHISNSVNLDLFSF
                     HWIDTSKEGISSFNQQFKKIFSQVGVSEEKTVIFYDNISGMLAARGVWLLEYFSHQNV
                     SILDGGFTKWKNEGRIWDTIPIGYKPTNFSGIPNSKILAGFEYILENLDKITILDARS
                     QEEFTGKLVRAARGGHIPTSINIDWKENISEQGVFKNDENLSELYQLPKDKEIITYCQ
                     GAYRAANTYLVLKKIGFTNIKVYLGSWGEWGNKLELPIEE"
     gene            24040..24372
                     /locus_tag="QGF01_00545"
     CDS             24040..24372
                     /locus_tag="QGF01_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HJN19426.1"
                     /translation="MASTRKKFSFLAVIAGAVMLVMTFATPSAFAAGGDDSGLSKPIL
                     ALAAAIAIAGGLIGTGSAQQGIGAAGMGIIAEKPEKFGQVLFFFVIPETLWIIGFVLG
                     IILLLDIL"
     gene            24384..24938
                     /locus_tag="QGF01_00550"
     CDS             24384..24938
                     /locus_tag="QGF01_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014093.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HJN19427.1"
                     /translation="MSIDTFIDEIETRKKNDLSDLDKKLNDKKSETNTKKNSVIKELK
                     DNYTNEAKTKAEREGARIVEAGKLEAKKILFDAINKNLDATFDVIKKELTGYSKKPEY
                     KKVLQKLVDYSKKVLAKEIVVHCRDEDKSFFKSGVKIGSTIQTLGGFVAENTEGTKEL
                     DLTFEELLRNNEDEIKNSILEKIL"
     gene            24938..26002
                     /locus_tag="QGF01_00555"
     CDS             24938..26002
                     /locus_tag="QGF01_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014094.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HJN19428.1"
                     /translation="MGASQYASSFGRLQSIAINFLSEDFLQNMLKANNVTEMVKQLES
                     TWYGEEIKKAASVYKDAALLEVALNRHIVETNKIISEATPFAGKNAVRTYLSKWDLYN
                     IELILSSKAMGREITETESLLVSSRNVPAGIAAGNISHDEIKIILAETTVDGVVNKLT
                     KYDYGVVLMKHLDEFQKTGDLGPMMSALHESYFQKLLESLKFFQGDEGAIRDLVRAEI
                     DKKNVLNLLKAKESNLEKDIVSKHLIDGGRISIKELLDSYEVKDVVEMVGRFDSYFKL
                     TDAIEQYKTSKSLIDFEVGITKNIFENNVKKLRNIALSIGNIFYFIFRAEIEHENLKR
                     ITYGKRYDLSIDKIKEMLLI"
     gene            25999..26358
                     /locus_tag="QGF01_00560"
     CDS             25999..26358
                     /locus_tag="QGF01_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HJN19429.1"
                     /translation="MSQQVSKQKTDSSGRLAVVGDRELVIGYRLIGIDDTFIVSDEED
                     ASKKIQELYSSGEFGLIIASNSMRAKLSSKFIAEIEESIEPLVLFMPSQKETAEEDKE
                     SIAALAKRVLGISIEVN"
     gene            26360..>26777
                     /locus_tag="QGF01_00565"
     CDS             26360..>26777
                     /locus_tag="QGF01_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HJN19430.1"
                     /translation="MVDATIARISGPVAVAKDLEGAHMFDVVRIGEMGLMGEIIRLEG
                     NTAQIQVYEDTTGLKPGEKVVNTQRPLSMQLGPGLLTSIYDGIQRPLNVLAEESGDFI
                     SRGKMIPALDQKKKWDFIPVKKNGDQVSPGEIIAEVQ"
BASE COUNT         9019 a         4609 c         4710 g         8439 t
ORIGIN      
        1 tcaatgaagt aattgaaatt ctttcaattg aaaaagtgga cgataaggac ttacgttaat
       61 ttcaatagta tgcggagtgc gggatttgaa cacgcgacct ccagcttggg aagctgacgt
      121 cctgccgggc tggactaact ccgcttactg attttagtaa gaatcttact aaaaacagta
      181 atcacatagc tgagattcat tctgatccag catacaagga gtggcttttt gaaaacaaaa
      241 acccactgta cgcaagatac atcttgcgtc gaagctcgcg tgttgacaca ttattaatgc
      301 ttaatattac atgtgagaat attgagtttc ttttaatgtt aacaagacta cattgctatg
      361 aagtatgatt tatgatgtaa tttatgagca tttaaaaaat attaggcaga ccaaggtcgg
      421 aacaattcaa agtgcagcaa caattattga tccaaagcac acagtttctc agaccattgc
      481 taaaattata aaaaatgact cgtacgatgc attttccaaa catggaaaga tagtatatgg
      541 aacaaatgtg agggagttgt tatctgcgag agctgttaat agtatgaaag tagagccatt
      601 tttacaccca attccatcag tgacaccgaa tgataatttg gaaaaagttg caaaaatcat
      661 caaccatcat agaacaagat caattccaat agttgaaaaa gatgagattt tgggagtcgt
      721 taaagcagag aacgttctca aggttctatc aaacttagac aataaatgga ttaaggctag
      781 tttaattttc actccaacac cgataactat agatcataac acgttactta gttctgcaag
      841 aaaaattatg atttcaaaaa gaattgatca tcttccagta acagacaacg gacaattaaa
      901 acagattttg acttcatatc atgtacttca aacactaatt cctgaagaaa gattagggaa
      961 aaaaacaatg gctgaaaaaa gaattacaag gtttggctca aaggtgaaaa acataggcac
     1021 aaacaagatt tcacaatgta aacctaatga taacatcagc acagtgatca aatctatgtt
     1081 ggagaataat acatcattta gtctagtaat gctatgggat aatattcagg gaataataac
     1141 ttacagagat attttggatc tgtttgagac caaagtagaa aatgatattc ccatttacat
     1201 tattgggatg ccagatggtg agagggacgc agacatgatc ggttctaagt ttaaaaaaac
     1261 tctgcaaaga ttaaacaaaa cttcgcctga aattcaagaa gcaagaatat caatcaaaag
     1321 acaaagaata tggggaacaa agaaactata tcaaatcact gcattaatta tcacaccaca
     1381 caaaacactt tcattcaaag aggaggaatg ggatctttca aaatcgatag aaaacattaa
     1441 tcaaaaaatt ctcaaaagtc tttcaaaaaa ggataggaaa agatcaaaaa ctagtataag
     1501 aaaaatcttg agataggctt tctattcaga atcttcaggt atatcgctag tcttactacc
     1561 tacatcaacg ctagaatcat tttctggtag ctaaaaacca aaaaaatttg atttagtttc
     1621 ttaactttaa atttgtaaag ataaggtcag attggtttga ataactaata cctgttcaat
     1681 actagtttaa aacgacttac taggtcaagc tagtagaggg ttgtttttgg acagtaagga
     1741 acggcaatat tcagaatcaa taaactacag tagaacaacc atagcgctat gacaaaaacg
     1801 acatcgcaaa aacacggtaa acgcggctaa aaacgtgatg aatttttaac cgtgtttgtt
     1861 cgtgtcaaac taaaattata aaatatctag tctcttattg tcacctaaaa tctcttaaga
     1921 tattattata gtatcgccta attttttatc aaaacaaaat caaacatgac gtaattgtct
     1981 agttttgtcg acttttttcg ccttaaacac atttgggaaa aataatcatg aattggtatg
     2041 atccagatca tcaatctaat ggagcaacat taggagacaa tataggacat caggaaaatg
     2101 atgactggga ttgtgaaata gaattaagtt gccataaaaa agaaaaccaa ggtagagtaa
     2161 gtacgatctt catgcgatat cttcgggata agtatggaca cacagctgat agagcattaa
     2221 aaagagcaca aaaacgtgaa caaagagaaa gacatacttc aacagaacca gattcacaga
     2281 ttatatcggg tgtattttaa aactttcttg ggttttctct tttttctttt cgtatttcca
     2341 gagaaccctt gattataaac ataataccaa ttgctaaaga accaagcaag taaggggaat
     2401 gaaatgcaat atggggtaat acacctaatg gtgcaagcca taaagtagaa agtggaggaa
     2461 ctaagaccac tccaatcaaa atccagaaca ctcctctcat tttcaatatc aatagatcac
     2521 ttcaatctaa tattaattca ataaaatttt aagaagctac aaattatcaa aatcaatgat
     2581 tcaaggtttt gcaactccag aaggaacagc aagttttgct agtaaagcta tagcacataa
     2641 ggagaatttt agaaagattc aaaatctaac attgtcaaac attggaatcg gaacatatct
     2701 gggcaatcaa gacttggaaa ctgacaaaca gcaaaaaact gcaataaagc aatccatact
     2761 acaaggagta aatgtaatag atactgccat aaattatcgc gcacaaaaat ctgaaagaac
     2821 cataggaaag gcagtttctg aattgattga agaagaaaaa attaatcgtg atcagatttt
     2881 tgtttcaact aaaaacggtt atgttacaaa tgatggtgac ataccagaag attttatgca
     2941 atatattatg agagaatttg gaaaaaaagg aatagtaaag gagggtgata tctcatcaca
     3001 gtataactgc attacattac catttcttga agatcagttg gcacgaagtc agaaaaactt
     3061 ggggttggat tgtatagatt tgatgtatct acataatgca gtagagggac agccacaaat
     3121 gcctcacgat gaatttattc atcatttatc agatatcttt gaattttttg agcagcaaag
     3181 agaaaaacaa aatatcaagt tttatggact tgcaacttgg gagtgttttc gggtttctaa
     3241 tgacaaacca aattatttat cgttatcaga agtaatcgaa ctggcaaaaa agattggtgg
     3301 agataatcat ggatttaggt ttgttcaact tccatacaat cttagttttg accaagcatt
     3361 tatgcaaaaa aaccaattag cagactctaa taaccaaata acatttttgg aggctgccat
     3421 acaccataat attggagttt ttactagtgt tccactgatg cagggtaaac tattagaatg
     3481 gattaagaac aatgaaaaat ttacaaatca aagcccatct gtagatgcac ttcagtttat
     3541 tagatcaacc ccaggagttt tagctccatt aattggtcag aagcaggaat ctcatgtatc
     3601 tgaaaattta caggtgttaa aaattcctcc ttataccgaa caagagtttt atgatctttt
     3661 taaggaacta actggatgag ttatggtaat tcctcttcaa catcagaaca atgtttgtga
     3721 acccatacac cctttgagtt ttttactatt tctttacctt ttttgattga ttcattacat
     3781 tctacgcatt ttccattaaa tctagctttc aaagtaacag tattacatct atacctaatt
     3841 tgaattagat gattaatttt tttgacgttc taactctttg gttgcttcct agagcattca
     3901 atacaaaact ctccaacctc gaaaacatac aggagttttt tacaatcctt acaccttctt
     3961 tctgctcttg ttttatcata accgtaaggc attgtatatc agtctgtaat tatgtaccag
     4021 aacccacatt tcgatttttt gagtttctag tttttgtggt tccccatgta ttctttttaa
     4081 tttgtgattt tgacaaatca ctagtatgtg ccttattttt atgcctctct aatcgttcat
     4141 tgctactaaa ttcgagattg catttgttac actttgtctg actttgatcc tttccaaaga
     4201 aacccaattt tgatcatatt atattatttt actagaaaat aaatgagatc actactaaag
     4261 atttttttca atgttttttc ggataatttt tacgatctta gccttggttt tacgaccagt
     4321 cttaaccaca ttctttgagt ctatcaaaaa aacctcgtta tagtcggagt tgttgtatcc
     4381 agaaccaata tcattggcta ccatcaggtc ggcgtcagat tccctcagtt ttttccttgt
     4441 ccgggaaacc aactcctttt tcgatatgtt agcttcagcc ttgaaaccga tgagaattcc
     4501 cttgtggatc tttttgatgt gatctatgat ctttggggct ctggtgagtt tgatggtagt
     4561 gttcctattg ctcttgatct tggagtttga gttttttggg ataaagtcag atacggcggc
     4621 agccaatatc acgacgtcaa atttctttct aagttctttc ttgaccgtac taaacatctc
     4681 tgaaactgta gttacacgaa taattttggc accttttgga ggttcagcga ttccaggccc
     4741 ataaactaat gtcactttgg cacctgctga gactagttca gaggccaaca gggttccagt
     4801 ctttcccgtg ctgatatttg tcattacacg tacaggatca atcttctcaa ctgtaggacc
     4861 tgcagtcatc aagaccttct ttccttttag tttttttgac tgtccgaact tggtcaaaac
     4921 aaaatccaga acatcttctg gttctggcgc ctttgccttt ccctcaatca tttgaggaga
     4981 tatgaaatcc accttccttc tcaaaaactg tatattcttg cgaactgccg cattttcgta
     5041 catcgatgcg tgcatcgcta atcccatcaa tataggaatt ttagaaccaa aggcaacggt
     5101 aagaaccgta gaaattggtg tgtcatctat accatttgcg agcttaccca aagtgtttgc
     5161 agttgatggg tatacaataa tcagatcaga tcttttgtaa tctgcaagat caatatgttc
     5221 catatttcca gttagtttag ttattacatt gttaccagta gcccatttca tataatctgg
     5281 tttgattagc tttgtacttg cgttactcat tacacatttg acatttgcac catgacgcat
     5341 caataatctt gcaagttcta ttgatttgta tgctgcaacg gagccagcca cacacaaaac
     5401 tattttcttt ccagataatt cagtaccata actttctaca atatctaagg atggatgatt
     5461 agtcatgact gtatctccat atgaaaagaa tgttcatcag atgggaatgt accactttcg
     5521 acatctttta cgtattgaga aatggatttt ttgatctctt ctgatagaga tagatatctc
     5581 ttagcaaatt ttggctttag tttctcaaaa agcccaagca tgtcatggat taccaaaacc
     5641 tgtccatcac aatattgtcc acaaccaata ccaattgttg gaacagaaag catcttcgtc
     5701 accttctttg tagcttcaga ggtaaccatc tctaatacta tactgaatac acctgcttct
     5761 tgtaacgctt ttgcatcatc aagtaaactt tctacatctg attttgtttt tccctgcaca
     5821 ctatactttg ttgccgtttg tggtaataga ccaagatgtc ccataactgg aatatcagct
     5881 tgtacaattg ctttaattat ttcaggcaat cttccttcta gttttactgc atcagctcca
     5941 gctttgatta gtcgttgaga atttgcaatc gcatcagtct cattttcata tgacttgttt
     6001 ggtagatcag caacaatcag tgcatttttt cttccattac taactccctc agtaaacata
     6061 cacatctgat ctatggttac aggagcggta ttttcatatc caagcatgac cattccagca
     6121 ctatctccta ctagaagtat atccacctgg tcacatagtg tggccattgt ataatcataa
     6181 cttgtaacaa ccgtaatctt ttgtccattt ttctttcgaa caatctcatc tatggtctta
     6241 ggcaagtcta gccctccttt tcaaattttt cttaatctca gagattgatt ctgccaaaac
     6301 ttccttgttg ttatatcttg gttttacctt tttctttttg caagctttaa caagcagttt
     6361 catcgcacgc gttacattat caacaattgt aacatcagac gcttgtgcag ttcgtgataa
     6421 tggatttaga tcaaaagtaa taactttctt ttttgctttc tttaatgcaa ttgttctatc
     6481 tccatcttct aaaggaacaa gaacaacatc agctgaaaaa attccatcct tgtctacgat
     6541 tcttcttgca ctgtctatgc cctttagttt tcttgcagat tttaggtcgg ttccaagaat
     6601 ctcttttgct ccagattttt tcaaaagtct ggcaattgcc ttctttcgac tttcaggtcc
     6661 atagaagaga tttacctcta tttttgcgtc taaaattcgg cttaatttga tgatttcagg
     6721 cgtacagaga gcagcaaaat tgccatttac agagatcact ggattcttgg cattttggag
     6781 agtataggcc gctgccgaga ttgccttttt ggcactctta gatgtctttt caccaatcag
     6841 ataatcaagt gcttcacctc gtccttgtgc aatcaatcct tcttttgcga ccaaaccaga
     6901 accaaatgcc ttgacaagtt tttcacgtgt ttgtaacgac acaaaacgtg gatgggattt
     6961 aggaatgttc atacaatcac aacaattttt gtttattcta gtctagcacc agaattatca
     7021 attctcgaag tgataattac gccgtcatca tattttttta aaatttccaa agccttttgt
     7081 ttcttatctt ttggaacaag agaaaatatg gtttcaccaa acaatgcaat tccacatttt
     7141 attccattgt catgaagatc tttaatcact ttgttcatct ttggagtaat tacatgaata
     7201 tagttagcaa actttatcga catatcttga aactcatcca tatcttgaga tttgacaagc
     7261 ttttgaacca tcttgcctcc aagaccattt atcaatgaga ttttttcctt gagaaatttt
     7321 ttagtagaaa ttgggttaaa acagataatc attacttcta gcttctcctt tggggttatt
     7381 ctctgaacct caccaactcc aggtgcacct gatttagttc taatctcaaa tccaccatga
     7441 tatgaagcta atacatcgcc aagtccagtc tgacatttta tctccgcctt gtgtgcaatc
     7501 tgtgcaactt ttgtttttgt gtatccatat ttcagtgcat catttaatgc aattgctaaa
     7561 gataatgcaa cagctccact acaaccaaat ccataaccaa caggaacatc aatctcatgt
     7621 gtcacatcaa aatattttcc atctaataga aactcattca aaacatattc tgatacacgg
     7681 atatcacctg tattgaatcc ctttactcta attgtataat tattgatatc gtgttccttc
     7741 tttgatctga cattaacagt tgtctttact cccttttgta tcgagaatcc agcccctaga
     7801 gaaccaagct gattcggttc ttttttatct aactcggctt tgaaaaatcc agttatgtga
     7861 gcaggcgaaa atgcttttgc acgcatgatc gagacgtaaa acctcttaaa atattaatac
     7921 ttggcttaaa tagtttaaat tagtataaat tgactacatt tacgcgacgc ctacagagga
     7981 ttggaagcag tatcctagtt tcattaccaa aagagtgggt tgttgcaaat aatctagata
     8041 aaagttcaca ggttgagatt gagacaacac aaaacaacct ctctattaca acacaacaaa
     8101 gtaaaaaacc aacaaaagaa gtggaaatcc catatccact ttcgaaagaa gatagtgttg
     8161 tagctaacat tactggtgca tacttgttgg gatatgatct tattagaata aaaggcaaat
     8221 ctacaatttc tattaaagat cgagaaaata taagagcctc aatgagaagt ctagtaggaa
     8281 ttgaaattat tgatgaggat gcaaaaacta ttactacaca aattttgctt gacgagtcag
     8341 cagtaaatcc acaaaaaatt ctaaagagaa tgagttctat tgctcatgga atgtttattg
     8401 atgttttaga tatgcttcaa aatcctgatc aactagcatc ggtggttgat cgtgatagtg
     8461 aaattaatag gcaatatttc cttcttgtaa gattaattcg tagtgcaatt gtagataaaa
     8521 gactcgctgc aactttcaat cttgtggata tggatatttt ggattatagg ttggcagcta
     8581 atataatcga gaatgctggt gatacgatag ttagtatcac cgacacaata gcaaaatcga
     8641 tggcatttaa gacaatgtcc aaatctcatt tcaatttaat ttctgatatt gcaaaagata
     8701 tagaggaaat acaaaccaag tcagttgacg catttgtatc aaatgatagg attcttgcta
     8761 ttactacaat tcaacatcat aaaaaacttt tgaaaaaaat gtacggtgca agaattccta
     8821 aaggtacggc ccttaaagaa actgagatcc ttccgttctt agatcttctt caaggaattg
     8881 aaaactatgc aaaatcttgg tctgacatat tggatctaat acaaccaagc tacaaataat
     8941 taaaataatt ttttttgtgc agcataaacc atcacggagc aaatcgtttt cgaatcgatg
     9001 atcttatcct tctttatcat attgatcaat ttcttcaaat ccatcttttt caccgtaatg
     9061 aattcatctg tatcaagatg agttttggac ttggttagtc cagatgctac aaagcagtga
     9121 attgcctctt gattatagcc aacagatgga taataggtga gaaaatgggt catttttttg
     9181 gccctatagc cagtttcttc ctcaatttct cgaagagcac attttcttgg agtctcacct
     9241 ttttccaaag ttccagctgg aatttctaaa atatatccat gtgggaagcg gtgttgcttt
     9301 acaagaatga cttttccttt ttcatcaaat gctagaatgg cagcagctcc tggatgttga
     9361 attatctcac gacgaacctt tcgtttttct atggtaagat tgtaaacatc aatgctcact
     9421 ggctttcctt tatagacagt cttttttttc atgttatgtg gttattctta acagatgaag
     9481 tctttcagat ttacggtttt ctttgatcgc attcctgatc caatcatgtg gaaggctaat
     9541 cttttttggc atgaaatttt caactgcaac cacattttca acagaactag ctttctggac
     9601 catctcatcc atctcaaata tatcttcact atacaaaaac aacgcgatct ggttttttgc
     9661 aataaatgga atttgcataa agtgatcctc aaattgtttt tccaatgttt tcagtaaatt
     9721 ctttatgttt ccgttaacca cacataatac tgcatgtgaa atgtatggtt ttatttttgc
     9781 aggattgtat attagagtaa actggaatgc aggattttcc tgaagctttt ctatagatct
     9841 ggtaactgtc ttggatgaga tctttgcatt tttagcaaca acatcaattt gctctcgtgg
     9901 atttttcaat aattgctcaa tcacatcaag atctgttttg gtaaggtttg attctattcc
     9961 agcatcttct gcttcaaaga tatttagaat tctaacttgg tttagcaagt ttttgattat
    10021 agcaattttt tgctcaactt caccatttac aacaattcca caggctgaat aacctccaac
    10081 acatggcaca acaaaaaatg gttcaccaac aagctttact tgcttcatta tctcatttac
    10141 gtcctggccg gttgtgacaa gatagattac attatatcct aatagatgtg gttctatctt
    10201 caaagtaaat ttttcaatca gctttgactt taacatttta ttaattctag actttactgc
    10261 aaccccagaa agtccaatct tttgtccgat ctgtctatct gattccctac aattatttaa
    10321 taaattgcta agaattctca tatctaaatt gtctattttg ctaagtttca ctataatttc
    10381 atattaaata gtagttatta tacttatttt tgtctaatat gttagcacta tggtaataat
    10441 cctttatata ttctacataa attaaacatg cataatggat tataggctta acctagccaa
    10501 gagaatgctc tttaacaaaa gaggcagtct gattggggct gtactggctg tttcgattgg
    10561 aattttagta atcaatgtaa attttgtaat ttttcagggg atttatgatg cgataatacg
    10621 tgatatgtca gattatcgtt ttggagacat ttacgtttac gatgatgaga attccactat
    10681 tgaaaaatct gatattgttt ttatcaattg gtttgaacga atgccaaatg ttgaggcagc
    10741 aacgccgagg ttagaaggat ctgctacagc agaaactaga attttaggac aggcacatga
    10801 aaagtttaac gtaccagttc ttggtgttga tcctatttct gatattagga catctaccat
    10861 ccacgaaaca gtaggtgagg gacagtatgt ctattctcgt aattcgattg ttgttggtga
    10921 atcagttgca tctgatcttg gaggttgttc acaagatgga tttttcatgt ctggtggaac
    10981 tgatccaaca gtaacaggga gtgaagccat cagtaaatgt caaccagtta gggttggcga
    11041 cagtgtaaaa cttatcataa ccgatcagtg gggcattgac caaaaaaagc ggttcgttgt
    11101 aactggaatt tctggaacag ctggtggcca gggatttgat aggagcgtca ttatccacat
    11161 agatacttta cgtgacatgt tggatagatc aggtgaatca agatcaatta tggttagatt
    11221 acaagaatca acaactgcat atgaagcagc acaaattaaa aatcaatttc ttgcagcatt
    11281 tccaaatgat aatctaaaag ctgaaacgat tgaggaatct gcggaatcaa ctctttcagg
    11341 attcagatct ggaattgcaa tgattaattt aattggctat ttcggaatgg gttcatccgc
    11401 atttgccatt cttactatac aaatgatgtt agtaaacagc aaaacaaggg aaattggtat
    11461 tatgcgagcc attggtgcta gacgcaaaga cattttgatt atttttatca tgcaaggaat
    11521 gattattggt gcaatgggtg caggtgcagg taccgcacta ggattgggtt atactacata
    11581 tgccaaaata acaaatatgg aatttcaggg cagtttggca ctagaggtta actataactg
    11641 gcaaaaaatc ggtgagacag cttttctagc attcattctt gctatgattg cgtcaatata
    11701 tccctcgtat cgtgctacta aaaaacaacc agtggaggcg atgagaactg tctgatgttg
    11761 ctttacaatt agaaaatgtg accaaaatct acggagaagc agaaaccaag gttatggcac
    11821 tcgatgatgt ttcattttct gtaaaaaaag gcgaatttgt attgattgta ggaagctctg
    11881 gttctggaaa atctacccta ctaaacatga ttgggttatt ggacaagcca acaaaaggta
    11941 aggttagcat agatgggttt gctacaagta atcttaccga taatgaaatt tctgcattta
    12001 ggaataaaaa attgggcttt gtgtttcagt ttgctaatct tttggcagat ctcacagtat
    12061 tggaaaatgt cttacttcca agccaaatac cgttagattc ttcaattcct tttgctaatg
    12121 caaagaaaat tctagaaaag gttggtctag aagatcaatt atacaaaaga gcaaataaaa
    12181 tttctggcgg ccaagctcaa agagctgcta tcgcacgcgg acttgtaaac aacccaacca
    12241 tagttttggc agacgaacca acaggaaatt tggattcagt tactgctcaa aacattgttc
    12301 aattaatgaa gaaaatggca aaggaactaa accaaacttt cattgtagtc actcacgaca
    12361 gaaaacaatt tggagatgtt gatcgaatta tcactgtcaa ggacggtcac gtatttgaag
    12421 gcgaacatat ggaacaaatg gaggtcacaa tatgaattac aaacttttct tccttgtaac
    12481 aattttcttg tcaggaactt gtgtatccag cattgacgct gaaatccaaa cgccaccgca
    12541 aggagttgta actgactcta aacggataat gggcgtaaaa tttcttgacg catatttcgg
    12601 aacagaaaca actaagatag aagttgcacc aggagacaag aatgtaccgt taacagtaac
    12661 attggctaat ataggaacta acgatattgt cggtattaaa ggacaattgt atttgccaat
    12721 gggattctct tcaccgaatg gcgcttacaa gccaatatat gcagatacta atcaaaaggc
    12781 tcttgcgggt gctaatttcc aactaacatt tttcgtaaac atttctctgc aagcagatat
    12841 caaaacatat cctgcaacta tagagctgga ttattcaaga ttacgagaat ctggacttca
    12901 tcacgatgca tttaattttg atttcaaact aactggtgag agtgtaatta atcttaaagc
    12961 tgttacacca tttatcacat caatcacaaa taacaatgta gttttagaaa tttcgaatcc
    13021 aggctcctct agtttatcaa atattgatat agttctccaa aatacttcta caacaacttc
    13081 ttccacaact acatccacca caaatgtaga gaaagtaatt tttgatcaaa atcagtggaa
    13141 aattggaaat atcggaccaa actcctcaaa gatattttca tttaacgtgt ttgttccgga
    13201 aagtttgaaa aacgaaccat tacgtattcc attaggaatt acttattctg acgcacatgg
    13261 agaacaaaaa tctgtaacta gggttgcaga tctttacatt aacggattag tcaacccatc
    13321 aatatatgga gtaaaagtaa ttgaattgtc aggaaagaaa actattattg gtgagatatt
    13381 aaacgaggga aactctgatg gattatttgg atttgtcaaa ttacagccac gtggtgattc
    13441 aaacatcaag gaatcatctc agtatataga tgaaatagaa cctgactctc cagttccatt
    13501 taacatccca atagaaacta acggaattct aagttttggt gaacatgata ttaggatact
    13561 agtttcatac aaagattcag tacgtgatga acatatacta acatacgata ccaccataac
    13621 catcaaacca tttgaagata atactgatta tggttctatg ataggtggac tgatttttct
    13681 tgcatttgta attgcggtag cttacaaact gtatagtaaa aataagattc catttatcaa
    13741 aaaaggacaa attccattca ttcataaaaa aatagattcc taggaaatat agctaaacaa
    13801 tataaacaat tagagaaata ctcgatcatc aaaatgaaag agatcgaagt tccaaagaat
    13861 ctaagacaat tcatgattga aggagcagaa gagaccaatc ttggtgataa aaagggagcc
    13921 agaaaacaat tccgttatgg aaatctacac attcgtgaat atgatgatag gttcacagtt
    13981 cattcagata aaattgatcc tagaaaaaat ccattaggtc atttgttaat ggatgcaccg
    14041 gaagttttga ttggtctagc aggagctgct attggtggat tagcaattgg agcttacatt
    14101 tacaaaatga aaaaagactc tccagtcaaa acaccacaat caataatctc tggcttagct
    14161 gcatccttag caacaggtta tgcgtcatac attatttcaa aaaaactaaa agagtaacat
    14221 cttggtactt gtaagaacaa ttcacgtgtt tatcaaacta gttccagtta tactagctct
    14281 aagaaaagat cgaattcttt ggatcgcaca ggaaggaaag ggcatagacg aaaagcgatt
    14341 tagaagaaat gcacaaagaa ttctaaacac atgtatttca ctaggtcctg tttttataaa
    14401 atttggacag tggctttcgt cacgtgctga tattctacca caaccctatc tagaagaatt
    14461 atctaaatta caagatagtg ttccagctgc tccatttgat aaagtaagac cgataattga
    14521 gaaagatatt ggcaaaattg aagagaagtt tgatgatctc gaccaaaact cactatctgg
    14581 cgcatcactt ggacaagtac atcgtgcaac aaagaatgga caacaagtca tagtaaaaat
    14641 aaagcgacca gggattgaaa agcaagtaga aaaagatctt aaagttttga tgaagataat
    14701 cccatttgca atgaagtttg ttgatccaaa tttgagattc tcaattattc caataatgaa
    14761 acagtttgca gactcgattc atgaggagat ggactatact aaagaattgg ataatctaaa
    14821 aaaaataaaa aagaatatga agccatacaa taacgtcgta attccagaag ttcatgatga
    14881 ttattcaaca aaaaatattc taacgatgga atacattcca ggaattaaaa ttaccaatat
    14941 tgaagcgctc gacaaaaagg gaattgatag acaacagttg gttattgacg tgcacaaggt
    15001 cttttttact atgcttttgc gacattcaat atttcatgcg gatccacacc caggaaacat
    15061 ctcggtgaag gatgacggat ccctgatttt atatgacttt ggaatgattg gaagattgaa
    15121 taatgagact agattgcgtc ttgtgcgact ttatcttgcc cttgtggaaa aagatcctcc
    15181 aagaactgta aatgcaatgg atgatcttgg aatgcttgca ccagatttta atcgagaagt
    15241 gatagaaaag ggaattgaca tgtccataaa atcaatgtat ggtaaaaagc cagacgagat
    15301 ggaagtagaa gctttgatgt ctcttgcaaa tagaacaatg tcaaaatttc catttaaact
    15361 tccaaagaat cttgcacttt atcttagaat gtcaaccatc atagagggaa tctatcacac
    15421 acataaggtt gattttaagt tcatcaaagt tctaagacaa attttagaag aggaaagttt
    15481 gatcaaagat gcatacattg aagagatcaa gcattcattc aaaagatttg caaagacact
    15541 tgatgatact ctcacaattg cgccagagat aaaaaaattc atggatgaga atcgtgttct
    15601 aatgcaaaaa aacaaacgtg gttcgaacac attactttct ggaagtatct tatctggcgc
    15661 tgtgtttttt ggttctgcat ttctgttcca atcaagcgaa accatcggaa cgattggaat
    15721 tattgcatct gcagcaatca tgggaatatg tgtagcagct agaaatcgtt aatctaatga
    15781 gatttctttt cccttctttg taattgggat tttaattgtt agtacaccat taacatattt
    15841 tgcagaattt actttctctt ctccctcttt cacagttatt ggtagtctca tttttttctc
    15901 aattatacgt ggtctttgtt tcattattat tctaccattc acatcatttt tcttttctgc
    15961 tttgatagat aagacatttg agttcaatgt aacattaata tcgtttttac caaatcctgg
    16021 aatatccact gtgactatga gagtatcatt ttcttcatgt agatcaattg ggggcaatac
    16081 aaattcaaaa aattccctag atttgtcatc tatttcttta aaaaaatctt tagctatatt
    16141 ttttactaaa tccatttctt ccaatgaatt tttttacatt ataaattttg atagattttt
    16201 aatcatgaga tgttaatttt acaatatgat tattgcaatt gaaggtagcg atcaggctgg
    16261 aaagaaaaca caaactgcaa tgttagccaa ggcattaaaa tcgcaaaaaa tcaaaacagc
    16321 aatatttgat tttcctgact attcaactgt aatcggtaaa gaaattgatg cttatcttca
    16381 cggcaaaaga aaatttccac cagaaatcat tcattatctt tatgcagtaa atagatggga
    16441 aaaagtggaa gagattaaaa aatcagctac aaaaaattct attttaataa tgaatagata
    16501 ttatcattct aacattgttt acggtattgc aaatggatta aaggaaaagt ggttagaaag
    16561 cctagacgcc agtgttccaa aatctgatct tgttattgtt ttagatgttt cacaaactga
    16621 atcattttca cgtaaaaaat ctaaaagaga caaatttgaa aaaaataaac tctttttaaa
    16681 aaaaatatct caaatatatc gtaaactagc taaaaagcac aagtggaaga taattgatgc
    16741 atcaggtact aaacaggaaa ctcataagga aattcttaaa atcattttta aaaaaatagg
    16801 atcatgaaaa aaacatattt agacataatc gatccgattc atgattttgt acgagttaac
    16861 aatagtgaat tggaaattat tgacacacca atcttccaaa gattgagaag gataaaacaa
    16921 ctttcaggtg ctcatttgat atatcccggg gctcagcata ccagatttga acactctctt
    16981 ggtgttatgc atattacatc tatggcgtgt caatcacttc ttgaaaaatc aattattcca
    17041 ttagacgaaa tccaaaattt acggtttgct ggattattac atgatatagg gcatggtcca
    17101 ttctcacatc tttttgaaga ggtattacaa aaaaatcgaa aaatatctca cgaagatctt
    17161 ggaaggaata ttatacaaaa gacagagatt ggtgatctta tctcaaaaaa tggatttaat
    17221 aaaaaatcta tagcaaatct tgctttcagt aactccaaac atcaattcat gaatgagata
    17281 atttctggtg ctttgagcgc agatatgatg gattatttac ttcgagatgg atatttcacg
    17341 ggtgctgaac atgcaaaaat tgatcacaag cgtctgacat attctttaga tgtttacaaa
    17401 aataaactcg cacttgataa atctgcattg gtaaattttg aaacgatgtt gatttccaga
    17461 tatcaaatgt tcaaagcagt atattttcat aaaactgtga gagctggtga ggtaatgcta
    17521 ctaaaagcaa tgagtttggc agaagatgaa ctaggttttt cttcattaga tctagacgaa
    17581 tttctaaaac ttagtgatga atctgttctc tctaaattac ttaatctacc agaatctaat
    17641 tctgatctca aaacttcaaa aaaaattgcc actaattatt tgaacaggaa tttattcaaa
    17701 tgtgtatttg aaagaatggt aacatcaagt cagggattta gtaataaaat gattggcgaa
    17761 ttagagaata aaattgcaaa aaagtcaaaa attgactcaa atgaaattca tttagataca
    17821 tctactacaa attcaatccc aatgacacca tcaaaaaagg aatcaaaatc agtaattttg
    17881 ataaccagaa aaaataaaat tatagcggaa gaatttccaa tttcaaaaat tccattggtt
    17941 tctacaatat ctggtaaaat gaacattctt agagtatata ctcaacaaaa taaccgcaaa
    18001 aaagttgaaa ttgccgctaa atcaatactt agtgaaatag aataatgaaa aaaaaattag
    18061 tcataaaact ttctggtaaa gtatttgcca ttgaggaaac aaagaaacta aaagattatg
    18121 ccacattttt tgtaaagaaa agcaaaattt gtcaaccaat aataatagct ggtggaggaa
    18181 agattgcgcg atattttatc aaccatgcta gatcttcagg ggctgacgag tccacgctag
    18241 atgaacttgg aattgaaata tcaagactta atgcgcgatt actgatttat gcattaaagg
    18301 gtaaagccta ccctcaccca cctacaaatt taaccgaagt aaaacttgca gtagatagtg
    18361 gtttaattgt tgttacgggt ggattgcatc caggtcaaag tactaatgca actgctgcac
    18421 ttattgcaga aaaagttcat gccacagaat ttctaaatgc aacagatgtt gatggaatat
    18481 atgattcaga tccaaacaaa aataaaaatg ctaaaaaatt caaacgaata gaattaaaga
    18541 atcttcgtaa tttattggtc catgaggatt ctgtagctgg aggttatgat cttatggata
    18601 ttgtggcatt gaaggtgata gaaagatcca aaattaaaac aagaattatc aaatcagata
    18661 tcaaaaccat tgaaaaagct atgaaaggat tgtctacagg aactgaaata atcttaccta
    18721 attgattatt ttaattcatt ttgtacagtt gggtatacat cattccaaat cttaatttta
    18781 ttatctgaaa attcagctat acctgaatcc aaaacttttt ggaaaacact ttttgcttca
    18841 ccttcatcta acggttttgt ttgtgctttt gtaaatccac cttcatctac taatattatt
    18901 acaaaaccat cctcagtttt tattactgca gcaaatgatt cttcacctac gggaaaaaat
    18961 tttccatcaa tttttttcgt tgtcagagtt aacatagcaa aaccagttat ttcaaacaaa
    19021 gccatttgtg ctttatttgc cacccgcttt ccaatttgaa cagcttgaaa atactgcatg
    19081 attttatcaa actatcgtag tataagcatt ttatcttaac acttaagaaa tgtactaaaa
    19141 aaactgacat atgaatccaa gaatatttgt tggtgttgcg gttgtagcac ttgtagcagt
    19201 tttgtttcta tttgggtttt cttcggtaat cagcgatgtc agtgaagatg gatttttcac
    19261 ttcaccctcg gatctgggac aaacagtttt accacttgag attgatttac atgatttttc
    19321 catattagaa gtctcagaaa aacaggcaac tttgcatgtg gaatttaagg ttacgaaccc
    19381 aaatttcaaa tcagttatgc ttcaactaat caaatattct atttatcatg atgatgaatt
    19441 aattgaggtg ggtgaaatag gtacaagacc agagggtggc attacttcac caaattattt
    19501 tatcatactt aatgaaagac cattgccaat aggcgaaaaa tttactatca aaaatacagg
    19561 taatactcct gagctttggc aggcactaaa taatgatgaa ttaaactgga gagtaactgc
    19621 tgagatattt tataatctta gttcattgac atcaggacaa gaaaatacat cgacttttga
    19681 tttcagcaaa caggactaaa atgtatcata aaattatgaa attgtgatta tttgtgaaaa
    19741 tataagaaaa attaacaata ctatatatga tggaagctgg caaattcaac aaatggcaga
    19801 agcatcaatg gcagcattcg ggtcagcaat aggagtttta atcgctatgg cagttgtaac
    19861 atttgcactc cgtggcaaag gacatcctga agcaccagac gcctcgtaaa aaaaaattag
    19921 aaaacaccct acattatttt tctaatcttt actaccgaac agatttctaa aggtaattgg
    19981 tttacctaca ttatttttct aatctttact accgaacaga tttctaaagg taattggttt
    20041 accttccttc tttttgatat aacatctaga gtagtattga caagtattgc aatccactga
    20101 tggttctatt atgggcggct tggcttttgc atcactattt tgcctcttta gtaaatcatg
    20161 taataccata gctcttctca caacctcttc aaacatcttt tgatttctat ttaccacaaa
    20221 cgaatcttct tttccgcccc ttgtgatata tgtaattata ccttcaattt tgtcaaagat
    20281 ccacatacat gcatttagat ataaaatatc attagctagc ggactttcag gaaattcttc
    20341 cacagatcga aaaatcatga taacatcact ttcaatcata tccgcttgtc cttttagttc
    20401 cacaccatct ttcattttgt aattatttgt cgtggctttc gatttcatct gtcgaaaaac
    20461 tccacctaga acttggttta aattactttc ttctacattg ataggatcta ctctatcata
    20521 gtaagatttt cgtaaacatt ttgtaacttc atggagaaga attgtgttga catccttagg
    20581 gtctatatct atttcaatgt cttgcctgat accatctaga gcgtttttaa cgatttgttt
    20641 gtagtcttta tctccagcac caccgccagc aaatttagga gtcattattt tcagctatat
    20701 ttggtacctt aaatagatta accacaactt atttccctat gaacatattc aaaacagcgt
    20761 ttttatcaga ttttgataaa gtcgtaaatc cttctaataa ctccttttga atcttgaagg
    20821 tctggccaga tatttttttc atctcaataa tattgaaagg tataatgtcc ttcacttttc
    20881 catcacaata tgcccttatg tatttctcaa aaattgagta cgcaaatgaa ggtgaaattt
    20941 tggctaatat ttttattgat tctttaaaat gattctcttt tatttcagat tttgacaaaa
    21001 attgctgcaa cacaccatct ttatctttta attcaaccag gcctaatgca acaagaagtc
    21061 cctttattgt taatttgtaa aatggaacac ctgattcttg caaagcctta ggtccacgag
    21121 ttagtggtaa ccgtccatct tcgatgacaa tattcaatgg aataaggact tcatctaaat
    21181 cccgaaaaac tccagaatag atattcttcc aaactatacc atttttttcc gctatgtgct
    21241 gagcaattgc agtacgtgtc cttgtaatag aatccccctc tgaagcaagg tgagaaatga
    21301 tatatctctg ccttttagcc tcaccagtca attgatcagt ttttgtttta aatgtatgaa
    21361 atatagctag attgtttgtt gtattcttca gtaaggttca cctttcatac ctatttctaa
    21421 tataacataa tattagaatt ttgtgatact atcttaattt aaatatcttt agtaaatgac
    21481 ttcatttacc attaatccaa ctttatgttt ataaccgcac tatcgacccg aaaaagaaga
    21541 tgaactctaa aaaccataag tatgctctat tacttatggc cgcagtcgca attaccgcga
    21601 caggagcaat gtctcaggca tatgcccaac agattaccga tggtatggat gggtatgttg
    21661 tcggagaccc tgatggcggc gccggaatct atactggaaa tcccaacgaa tgttggcatg
    21721 atacagatga cgatggcgtt ccagatatgt attgtctgat agatacaggt gatacagctt
    21781 ggatgataac ggcatcttct ctagttttgt tcatgacacc aggagtagca ttcttctatg
    21841 gtggacttgc aagatcaaag aacgctgtta acaccgttgg tatggtattc attgtcatgg
    21901 gactgatgtc agtccagtgg gtgctctggg gatactccct agcattcgga ccacatgata
    21961 acgaagcaaa catgttcatg ggcaacttag attacatggg tttcaatcaa gtttcagctt
    22021 gggcaccact aggtgaacca ggtccatgtg aaggcacatg gtctgattat tatcagatgc
    22081 aacaaatgaa atctgaagac gcaggtctat gtagtcaagg atggcctggt acagtaccac
    22141 atcaattgtt tgcaatgttc caagcaacat tcgcaatcat cacaccagct cttatcgttg
    22201 gcggtttaat tgaccgaatg aagttcagcg ctttgtgtat atttgttctc ttatggggaa
    22261 ccttcgtcta tgacccaata gcacactggg tatggggagg tggatacata ggaaacatgg
    22321 actttgatcc agacttatca ccaagctatg gacttgactt cgcaggcggc actgtagttc
    22381 acatatcttc aggattcgca gctctagctg gagctttagt tcttggtaga agacttggat
    22441 acggtaaggt tccaatggaa ccacataacg taccaatggt tgtactagga gcttcaatac
    22501 tctggttcgg ctggttcgga ttcaatgcag gaagcgaagt actatcagac ggaattgctg
    22561 ttagtgcttg gactgtaaca aacactgcaa ctggtatggc ttcagtaact tggttactga
    22621 tgtcatgggc acatactggc aaagctagta ttacaggtgc tgcaaccggt gcagtagcag
    22681 gattggtagc aatcacacca gcatctggtt gggttggacc aatggcagca atcataattg
    22741 gtgtcgctgc aggaactgtt tgttatggat gtgtagcatt caagaatgca cgaaaatggg
    22801 acgacgcact cgatgtatgg ggtgtacatg gaatgggtgg tttcacaggt gcagttttga
    22861 ctggaacact tgctagtcct catgtatggg ataccggtga cggtattggt gcatggactg
    22921 gaacaccaga aggatatgag caacaagcta tcaacatagc aggcgctctt ctctcagtgg
    22981 cttatgcatt tggtattaca atcgtaatat tgaagatcat ggacgcagtt tggccaggtg
    23041 gaattcgagt aactcctaaa gaggaggaaa tcggacttga ccttgctcaa aacggtgaac
    23101 gagcatacgt aaacgaatag aaaaaatctc tttttttctt tctttttttt cttttatttt
    23161 cctaaagaca aattcattta gtttcaattt tacacaattc tatgttcaaa acaacttctg
    23221 aacttgaaaa aatccttgat aatcccaatt tgctacttat tgacacaaga tcgttccaag
    23281 aatactctaa tggacacatc tcaaattctg tgaatcttga ccttttttca tttcattgga
    23341 ttgatacaag taaagaaggt atttcttcat ttaaccaaca gttcaaaaaa attttttcac
    23401 aggtaggtgt ttcagaagaa aaaacagtta ttttctatga taatatttct ggtatgctgg
    23461 cagctagagg agtttggcta ctagaatatt tttcacatca aaatgtatca atacttgatg
    23521 gtggtttcac aaaatggaag aatgaaggac gtatttggga tacaattcca attggttaca
    23581 agccgacaaa tttttctgga attccaaact ccaaaatttt agcaggtttt gaatatattt
    23641 tagagaatct tgataaaatt acaattttag atgcacgttc tcaagaagag tttactggta
    23701 aacttgtaag agcggccaga ggtggtcaca tacccacttc aataaatata gattggaaag
    23761 aaaatatttc agaacaaggt gtattcaaaa atgatgaaaa tctctctgaa ttatatcaac
    23821 tacctaaaga taaagaaata attacttatt gtcagggtgc atatagagca gcaaatactt
    23881 acctagtttt aaaaaaaatt ggatttacaa atatcaaagt gtatttggga tcttggggag
    23941 aatggggaaa taagcttgag cttccaattg aagaataaga aatgcgatcg aaaaaatcat
    24001 gatatagtta atataacgta aaaaacgcag gcaaaacaca tggcttctac cagaaagaaa
    24061 ttttcctttc ttgcagtaat cgcaggagca gtcatgttgg ttatgacatt tgctacaccc
    24121 tcagcttttg ccgctggtgg tgacgattca ggattatcaa aacctatttt ggctttggca
    24181 gcagctattg caatagctgg cggtttaatt ggaactggta gtgcacaaca aggaattggt
    24241 gcagctggta tgggaattat agcggaaaaa ccagaaaagt ttggccaggt cttattcttc
    24301 tttgtaattc cagaaacact ttggattatc ggatttgttc taggaattat cttattgtta
    24361 gatatactct agggcgaatt acgttgagta ttgacacttt catagacgag attgaaacta
    24421 gaaaaaaaaa tgatcttagt gatctagaca aaaaacttaa cgataaaaaa tcagaaacta
    24481 atacaaaaaa aaattctgta ataaaggagc ttaaagataa ctataccaat gaggccaaga
    24541 caaaagcaga aagagaaggt gcaagaatag ttgaggctgg aaaattagaa gctaaaaaaa
    24601 tattatttga tgcaatcaac aaaaatttag atgctacatt tgatgtgatc aaaaaagaat
    24661 taactggata ctcaaaaaaa ccagaatata agaaagtttt acagaaattg gttgattatt
    24721 caaagaaagt acttgctaaa gaaatagtag ttcactgccg cgatgaagat aagtcatttt
    24781 tcaaaagcgg agtaaaaatt ggttctacaa tccaaaccct aggaggtttt gttgctgaaa
    24841 acacggaagg aacaaaagaa cttgatttga cttttgaaga attacttaga aataatgaag
    24901 atgaaataaa gaattcaatt ttggagaaga tactataatg ggtgcatcac aatatgcatc
    24961 ttcatttgga agacttcagt caatcgcaat aaattttctt tctgaggact ttttacaaaa
    25021 tatgctcaag gcaaataatg tcacagagat ggttaagcag ttagaatcaa cttggtatgg
    25081 tgaagagatt aaaaaggccg catcagtata caaagatgca gcacttttag aagttgcatt
    25141 aaaccgccac attgttgaaa ctaacaaaat tatttcagaa gcaacaccat ttgcgggtaa
    25201 aaacgcagtt agaacttatc tttcaaaatg ggatctttat aatattgaac taattctttc
    25261 ctcaaaggca atgggaagag aaattacgga aactgaatca ttactagttt ctagcagaaa
    25321 tgttccagca ggaatagcgg ctggtaatat ctcacatgat gagataaaga tcattcttgc
    25381 agaaacaaca gttgatggtg tagtgaacaa actcacaaaa tatgattatg gcgtagtctt
    25441 gatgaaacat ctggatgaat ttcaaaagac cggtgatctt ggaccaatga tgtcagcact
    25501 tcatgaatca tactttcaaa aattactcga atcactcaaa ttctttcagg gagatgaggg
    25561 tgccattaga gatttagttc gcgctgaaat agacaagaaa aatgttctaa acctcttaaa
    25621 ggcaaaagag tcaaatttgg aaaaggatat tgtatcaaaa catctcattg atggtggacg
    25681 aatttctata aaggaattac ttgatagcta cgaggttaag gatgttgtag aaatggttgg
    25741 tagatttgat tcttacttta aactaactga tgctattgaa caatacaaaa cctcaaaaag
    25801 tttgattgat tttgaagtag gaattacaaa gaatatcttt gaaaataatg ttaaaaaatt
    25861 aagaaacatt gcattgtcta taggaaacat tttctacttc atatttagag cagaaattga
    25921 acacgaaaac ctgaaaagga ttacatatgg taagcgttac gatctttcaa ttgataaaat
    25981 taaggagatg ttattaattt gagccaacaa gtttcaaaac aaaaaactga ttctagtgga
    26041 agacttgcag ttgtaggaga tcgtgaactt gtaattggtt atcgtctgat cggaatagat
    26101 gatacattta ttgtcagcga tgaagaggac gcaagtaaaa aaattcaaga gctctactca
    26161 tctggcgagt tcggtttgat tattgcaagt aattcaatgc gcgctaaact ttcttcaaaa
    26221 tttattgcag aaatagagga atctattgaa ccattagtcc tattcatgcc atctcaaaaa
    26281 gaaactgctg aggaggataa agaatcaatc gctgcattgg caaaacgagt tctgggaatt
    26341 agtatagagg ttaattgata tggtagatgc tacgattgca agaatttctg gtcctgttgc
    26401 tgttgctaaa gatctagaag gtgcgcatat gtttgacgtt gtacgtattg gtgagatggg
    26461 tcttatgggt gaaattattc gtctagaagg aaacaccgca cagattcaag tttatgaaga
    26521 tacaactggt cttaagccag gagagaaagt agttaacaca caaagaccac tttcaatgca
    26581 acttggtcct ggactcttaa catcaattta tgatggaatt caaagaccct tgaatgtcct
    26641 agctgaagaa agtggagact ttatcagtag aggaaaaatg attcctgcac ttgatcaaaa
    26701 gaaaaagtgg gattttattc cagtaaagaa aaatggagac caagtttctc caggtgaaat
    26761 aattgctgag gttcaag
//
LOCUS       DASCPJ010000011        16008 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000150359, whole genome shotgun sequence.
ACCESSION   DASCPJ010000011 DASCPJ010000000
VERSION     DASCPJ010000011.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 16008)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16008)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16008
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000150359"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="QGF01_00570"
     CDS             <1..1091
                     /locus_tag="QGF01_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJN19431.1"
                     /translation="KIVSRHLGILAMTGMGKSNLVTLLAKQIAKLDGTVIIFDYHNDY
                     SGLDFPKINVIDAKINPRLLEADTLADVLEIREAADIQQRVLRLAFTPEVKESANFWE
                     ALDSQVQYVGANPDRKEDRHSADRVQDKIDDARHRASEILDPDMSNPVALIKEGRLNI
                     LNVSEFTDRQANVALAYYLQELLLDRKAAVNAKGGKVKSKRNYRFNSPVFVIIEEAHV
                     FIPKGEDAKAKYWAAKIAREGRKFGLGLGIVSQRPRDIEANVLSQMGSLAVMKIVQED
                     DQRQIASAAESISREFIAQLTSLNIGDAVLVGQWVNLPAIVHIDEMKGKKIGSDQNAV
                     DEWAVAKKVESAGTNSSQDLVQKDLLMD"
     gene            1093..2235
                     /locus_tag="QGF01_00575"
     CDS             1093..2235
                     /locus_tag="QGF01_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HJN19432.1"
                     /translation="MLFSHISDTHLGLQQYSLEEREQDIYDSFNQAIDISIKDHVDFV
                     IFAGDLFHTPNPSGAAILQMANALKRLKENSIESFFILGEHDISRMRATPVPYVYHNL
                     EFSKYVGRGKPVYHKDVMIAGFDKIRKNEISTFEEKFSEIDTLAKQHSGHKILVLHQG
                     ITEINQFAGELNSSDLPKNFTYYAMGHLHDKFVKQFEQLGGPLAYPGSTEMTTSEGIK
                     ETEKGFFEVDISSQESNPNWIKLDTRPQFSTKIEAKNLDASISEILEKISTFDKKPLV
                     EIKIIGKDIERDVVQTKISQIIPQTLHCSWKMLQNEDASSVLLNRPARIDEELFKLAV
                     NALKSEKLANFAVNDLLPLLSTNQLEQATQLVIENFEEFKGRKNDS"
     gene            2225..4306
                     /locus_tag="QGF01_00580"
     CDS             2225..4306
                     /locus_tag="QGF01_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN19433.1"
                     /translation="MIRNIELVDFLAHSNTKLEFDNDATVFVGDNGAGKSSIIDAITF
                     SLFGEHTRKNNKGLIRRGANQGFAKIEFTANGKNYQAIRKIDSKGALTAQFAEDLDGK
                     LIPIAEGERKQFGESMTKHVEETLGMDFEKLKIASIVQQGELSSIIKAKPKEFKELLN
                     TIIGIDKLDTALALMRTVQREFRTSIQEKFGYDDTQIQLIENKITEYENESENAQPKL
                     EHLNTEKKKQESLISKLEKQIQDDSTKESQLKDLESRKKEWKEYAMDIIKSIKKEVAE
                     KEEIVNECKPCFGLSKNKNEIELEINKIQKKILDTESELSDLVKKQVRLEEHGELAKK
                     LVLKDGKCPVCDSSVEHLKPLFQKKHIEDEIQEIEKKITQLENEKDELEENIGSLTGE
                     LEESKKAETILSTYKIKNESQLDEIVAEIKMKVKQMQKIPLTINSGQLVETASLDSHA
                     KTIYENIVSLEKSTKGFDQQQFLRNIESRDVSVSKLSQINQEYGEISGNIKATKIELE
                     KLSSTLAELKYVDGYVMELERIQENVYNRDGPVGKSLRSWALEIISQKASEYLEKLNT
                     KIQRISLSEKTRDVNISCYSRNTVLDLESLSGGEQVSVALALRLGMSHLLGASNLNFM
                     ILDEPTTHLDSERRKSLVNVLSQLTNLKDDDSSMQFIIITHDAEIFDDSAVENIYKFE
                     SAQNGTIVSRL"
     gene            complement(4303..4791)
                     /locus_tag="QGF01_00585"
     CDS             complement(4303..4791)
                     /locus_tag="QGF01_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HJN19434.1"
                     /translation="MDVVPQSRLSLLAEMEDKYEKKETEYFVDLLEHEDYVIRCRATC
                     ILVDIGGEDKVQYIAKVLKDDPNELVRHEAAFSLGQMCYSNGIEPLEDATKNDPSMFV
                     RHEAAIALGVMGSKKARETLEKALDDPDEPVRDSAVVALSNLEFMEKLSKNEKFAKLT
                     GG"
     gene            5068..5631
                     /locus_tag="QGF01_00590"
     CDS             5068..5631
                     /locus_tag="QGF01_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit C"
                     /protein_id="HJN19435.1"
                     /translation="MAQMPALIPKEVEIHRLKKIWLIVISMGSTAASVEVDNFVDGSL
                     HQTSIRDSAFTPAHWWLYSHFIALPLGWASVAIYDRKVPILRGPNNSMNTGLKMTILG
                     YLATMFTIGINEMWHFWFVEEIFAVPNHWMFNMGVVVAFMGALAYVVRVYARLVELGA
                     ETPGEDPYVAEMYKMALEGKLYSRSIP"
     gene            5745..6404
                     /locus_tag="QGF01_00595"
     CDS             5745..6404
                     /locus_tag="QGF01_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HJN19436.1"
                     /translation="MVVQVGQKAPNLKISQWVQGLDTNLDKQGDNIILVDVFQVNCPG
                     CFMYGIPESIEIYNKYKTEGVSVLGVATAFEDFDKNTISNLELLVKTGEVIGETKKML
                     SQYGKLDGNKLAYKIPYPVAMDSLIKESGDVSKEKMDSMINTQIPNFDAQSEDYKNQI
                     YERVKNYFMSKEYTAETFEMYSLQGTPSNILIDRKGVLRDVSFGQNGSLESKIQNLVN
                     E"
     gene            complement(6383..7759)
                     /locus_tag="QGF01_00600"
     CDS             complement(6383..7759)
                     /locus_tag="QGF01_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HJN19437.1"
                     /translation="MKVLFFLIPIILFVLITPTLVSAEVYIPDNEYVGFYDHDGVYTV
                     IGGIKNTEIYPVTPTITVTVHDGNNIFSNDYVFSSVMPQQMLPMKIKLPQITSDSPKL
                     EPYAITYNLTGYEFKGGYVLYNDSLVLHSDGNMTGQIKNSSDETFYDFKIYALIKDKN
                     EVILDVASSQKFDVMYPDEILDFQMIPNPKIANNIHLYSCFAFGDDEIFPLNAERNDE
                     KFTFRYDSGAWFTDGQFNQEGTALNMYALNSWHLDMNASLEFPQSSIHENLEVYLDGK
                     KYAEGQPIHEKIERLQSLDEMGNWHLYFTVPNFYQGDVTIKGFMHPDGTVSVPEELDL
                     SSMVMTELTSGEITGIIANTSENSIIFSLETDDSGILSMTTSDFLIRPFNDGSFFVLV
                     NGEQTENVKFENKILSIPYTSEAETIEVYGSYVVPEFGTIAMLIFAVAIISIIVVSKK
                     TTYSLTRF"
     gene            complement(7761..8219)
                     /locus_tag="QGF01_00605"
     CDS             complement(7761..8219)
                     /locus_tag="QGF01_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HJN19438.1"
                     /translation="MPNSQEQVWLKMLKNNSVELRQKAIIWRKENAITRIDKPSRITR
                     ARRLGYKAKQGIVVIRMRVGTGGMRKQRPRAGRRPKHLGVTRIKADVSMRKVSERRVL
                     ERYPNMKLLGSYFLYKDGMHYWFEIILADPSHPSIAKDKEMRRRVLPNIA"
     gene            8270..8920
                     /locus_tag="QGF01_00610"
     CDS             8270..8920
                     /locus_tag="QGF01_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19439.1"
                     /translation="MKIVVFSLSILVLFITLSSLTPAYSVPVVTIETSQETYHYCDKL
                     SITVNVSEITGDVATIYIINPEEGKSILLSPPISAETTELPSRFAFDATIWDPGTYTL
                     EIEYSETASSVQFSIDDNEEICLPYWIKDVAYMWISEPTVTDKDFARALGFLIEQEII
                     KIPYTSTGDETVTKIPDWVKTNAEWWITDQISDTEFTLSLQYLIEKGIILVNTSAV"
     gene            complement(8934..9602)
                     /locus_tag="QGF01_00615"
     CDS             complement(8934..9602)
                     /locus_tag="QGF01_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19440.1"
                     /translation="MKIAILLAISVLILSQGYGMAYGEKLTLSTDSSSYEKGTTIEVS
                     GLISDFDTSDPLKVYQITLRVVDPKNNIVTVSQFDPNDDGTFSTSINSDGPTWKFDGD
                     YVIMLNYGELKGTYTFAFSVPAPGSEIIDEAGDVIDAGVDVIDEAVDVADAIVEEVSG
                     ALQCGPGTTDDGGICIIDKSGSFNPQVEEGTFYAMIIPTAIAFGIGMAVLVALALIAR
                     AHRK"
     gene            complement(9604..10536)
                     /locus_tag="QGF01_00620"
     CDS             complement(9604..10536)
                     /locus_tag="QGF01_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HJN19441.1"
                     /translation="MDVKHTVLICDQVNSILNEILTKNGLEVTYEPEITPEQISEKIG
                     SYEVVVVRSRTKLVKELIEKADNCKIIARVGVGLDNIDQVAAKEKNIKVINAVEGAMN
                     AVAELVIGFMLSMAREIPRADREVRNGNWIKKELMGSELRGKYLGIVGLGNIGKRLAR
                     LARALNMNIIGYDVVPIDQEFSKEVGLMQADLDTLLSSSDYISLHVPLIDSTHHMINS
                     EKIAMMKKSAHIINTSRGGVIDEEVLYNALKNGDLSGAALDVFEAEPATSNKLISLPN
                     FIATPHIGAQTKEAQSLAANIIAEKIIQTIRGVI"
     gene            complement(10628..12064)
                     /locus_tag="QGF01_00625"
     CDS             complement(10628..12064)
                     /locus_tag="QGF01_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HJN19442.1"
                     /translation="MNKRINFMFTMAAIAVMGGALFGSSFTSQMTGSTFDFSKSDNAA
                     LQKIHDMGGLELVMPAAFAEAGNCDSDEYADRTVVEFPLTGVSIDLPRLGGGTFSAMT
                     FSEQIPGPTLRVTQGDVVKMTLTVPDGEPTPHGNDMHASQVTAVPTFGAVQPGTEKTY
                     CYIAQSPGVYKYHCSGINVAAMDQHVLQGMYGIAIVDPIDGYSKLMVEKTAVNANGDV
                     SRDRQFYSADALEFSLQYQQMYLTDAGTYDATKMFAHQTSYTAVNGQPFGYVPNEIHN
                     LLNNGDTGKNIFVAQPWNSMDLHQYQSQLMFTPTGVHNRIFVENQGNEPVYFHIVGEI
                     LDRVTQGNRVQSGATETWLLGGSQNMIVDIVYDEPGIYVAVNHDYAAIYTGAASIFVA
                     GLPLPNINQAATDLGVVQGAVDTGMYAGVLGNPYDSIPPYGSQSIHHPAKNVHCMCSD
                     GVAAAQAAEGAVELWVAVPAIIAAATGE"
     gene            12223..13584
                     /locus_tag="QGF01_00630"
     CDS             12223..13584
                     /locus_tag="QGF01_00630"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HJN19443.1"
                     /translation="MKNRSDKDSLGLVKIPADAYYGPFTGRAVKQYHVTGQKSHIHLI
                     KSFVMIKRSAAIANMKAKALDNKRGRAVVKACDKILAGKYQDQFVVDMINSGAGTAFN
                     MNTNEVIANVTLKILGKKLGQYQHLHPNDHVNMSQSSNDTYPTAMHMSILFALDDLVP
                     IIKKLETSLDKKAKQFEKYKKVGRTHLMDALPVTLGSEFKAYSSSVRYARLKIESVYE
                     SLYRVALGGTAVGTGANTPNGYRKNVINELARISSLPLKPAAESQHALQSRLAITHAS
                     SFLRNLAVELGKISNDIRLMASGPVAGFGELEIPAVHAGSSIMPGKVNPSLAECMNMI
                     CFDIIGNDTTVAFAAQAGQLELNVMLPVMLKAVLDSTDMLTNFLPIFTTNLIAGLGAN
                     KEKLQANIEKSPVIVTLLTPKIGYQKSAELFKESMKTGKTIRELVISKKLMTKKQVDS
                     LLS"
     gene            13587..14855
                     /locus_tag="QGF01_00635"
     CDS             13587..14855
                     /locus_tag="QGF01_00635"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HJN19444.1"
                     /translation="MAKIWVEAYGCSASFADSEMISGLIVNGGHTLTENESDSDLNLI
                     VTCSVKDATATKMVYRIKKSQSKPLIVAGCLPKAERHTVEKFAKNASLMGPNSIGKTV
                     QIIEKTLDGSKMVCLDDTDISKVGIPKVRLNHTVGIVEIASGCMSECTFCQTKLAKGD
                     LKSYRIGDIVRQVKTELADGCKEIWLSSTDNGCYGLDIGEDLSSLINNVSEIPGEFRI
                     RVGMLNPMFIPRIRNNLLKSFENDKVFRFLHVPVQSGSNNVLNNMKRGHTVETFKNVV
                     KEFRTKFGPFTISTDIIVGYPTETEEDFQETVKLLKETKPDIINLSRYSRRPGTVAAE
                     MPQLNVAEVKRRSKQITDIVNNISLENNKKWIGWKGKVLFDEDTDGQIKGRNFAYKPI
                     FVENDAKLDQISTVLITNATNHSLVGEIAS"
     gene            14928..15869
                     /locus_tag="QGF01_00640"
     CDS             14928..15869
                     /locus_tag="QGF01_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HJN19445.1"
                     /translation="MTFEITEPILVIGLGRVGADLAEKAKKSLNSNSLLISHDQKDLT
                     GENSIKISTKSVVNPSIHLIRGSTLENLEEIEKNISKYSTIILMSNLAGKAGIGIAPV
                     VSKICKEQEKNLLSFAIMPFKFEKDRIFQSGIALKRIRHDSHCTIVIDNDALLDSNPD
                     LSHEQCNNISNKAIESVISSLKSSEISEDVNILSTSKNASDMEVSLKDSLRMLYEDAP
                     PNSIKRSMLYVYGGSNVPIGVLNSISDITGGVFDENTTHVDMSSNESKIVMLSSVQGE
                     TKFDRYDPLGIISSEKTIDWDEPECSINCELDLKQLE"
BASE COUNT         5450 a         2794 c         2916 g         4848 t
ORIGIN      
        1 acaagattgt ttcaagacat cttggaattc ttgcaatgac aggaatggga aaaagtaatt
       61 tggttacatt acttgctaaa caaattgcaa aactcgatgg aactgtgatc atttttgatt
      121 atcataatga ctattctggc ttagattttc caaaaattaa cgtgattgac gctaaaatta
      181 accctagact attagaggca gacacacttg ctgacgtact agaaatccga gaggcggctg
      241 acatacagca aagggtactg cgacttgcct ttactccaga agttaaagaa tctgcaaatt
      301 tctgggaagc attggattcc caagttcaat atgtcggtgc aaatcctgac agaaaggaag
      361 atagacattc tgctgataga gttcaagaca aaattgatga tgctagacat agagcatctg
      421 aaattttaga ccctgacatg tcaaacccag ttgctttaat caaagaaggt agattgaata
      481 ttctaaatgt atcagagttt acagacagac aagcaaatgt tgcgcttgcc tactatctac
      541 aagaactttt actggataga aaagcagctg taaatgctaa aggtggaaaa gtaaaatcaa
      601 agagaaatta cagattcaat tcacctgtat ttgttataat tgaagaagca catgtattca
      661 ttcctaaagg tgaggatgca aaagcaaagt actgggctgc aaagattgct cgggaaggaa
      721 ggaagtttgg tcttggtcta ggaatagtat cacaaagacc aagagatatt gaggctaatg
      781 tcctaagcca gatgggctcg cttgcagtaa tgaagattgt gcaggaggac gaccagagac
      841 agattgcatc tgccgctgaa tccattagta gagaattcat tgcacagctt acatcattga
      901 acattggtga cgctgttctt gttgggcaat gggtaaacct tcctgctata gtacacattg
      961 atgaaatgaa aggaaagaag atcggttctg atcaaaacgc ggtcgatgaa tgggctgttg
     1021 caaaaaaagt tgaaagtgca ggaacgaatt caagccaaga tctggttcaa aaagatcttt
     1081 tgatggattg aaatgttatt ttcacatatc tctgatacac atttaggatt acaacaatat
     1141 agtttagaag aaagagaaca ggacatctat gattcattca accaagcaat tgatatttca
     1201 ataaaagatc atgtagattt tgtaatattt gccggagacc tgttccatac accaaatcca
     1261 agcggtgcag caatcttaca aatggctaat gcactaaaga gactaaaaga aaactcgatt
     1321 gaatcatttt tcattctggg tgaacatgac atcagcagaa tgcgtgctac gcctgtacct
     1381 tatgtgtacc ataatctgga attttcaaag tatgtaggaa gaggtaagcc tgtatatcac
     1441 aaagatgtaa tgattgcagg atttgataaa ataaggaaaa atgagatttc tacgttcgag
     1501 gaaaaattta gtgagattga tactctcgca aaacaacata gtggtcacaa gattctagtg
     1561 ttacatcaag gaattactga gataaaccaa tttgctgggg aattaaattc ttcagatttg
     1621 ccaaagaact ttacttacta tgcaatggga catctgcatg ataaatttgt aaaacagttt
     1681 gaacaattag gaggacctct tgcatatcct ggatcaacag agatgacgac tagtgaagga
     1741 ataaaagaaa ccgaaaaagg attttttgaa gtagatattt catcgcaaga atcaaatcca
     1801 aactggataa aactagatac cagaccacaa ttttctacaa aaatagaagc caaaaactta
     1861 gatgcttcaa tatctgaaat cttagaaaaa atatctacgt ttgacaaaaa accgttggtg
     1921 gagattaaaa ttattggcaa agatattgaa agagacgtag tacagacaaa aatttcccag
     1981 ataattccac aaacactaca ttgttcatgg aaaatgctac aaaatgaaga tgcatcatca
     2041 gttctactaa acagacctgc acgaatagac gaggaactat tcaaacttgc agtaaatgca
     2101 ctgaaatcgg aaaaacttgc taattttgct gtaaatgatt tgcttcctct attatcaaca
     2161 aaccaattgg aacaagccac acaactagtc attgaaaact ttgaagagtt caaggggaga
     2221 aaaaatgatt cgtaatatag agctagttga ttttctagca cattctaata caaaattaga
     2281 gtttgataat gatgcgacag tatttgttgg tgacaacggt gcaggaaaat caagcatcat
     2341 agatgcaatt acattttcac tatttggaga gcatacaaga aaaaataaca aaggattgat
     2401 tagaagggga gcaaatcagg gatttgcaaa aattgaattt acagccaatg gaaaaaacta
     2461 tcaggcaata agaaaaattg attctaaagg agcactaact gcacaattcg cagaggattt
     2521 ggatggaaaa ttaattccaa tcgctgaggg agaaagaaaa cagtttggag aatcaatgac
     2581 caagcatgtt gaggaaacac ttgggatgga ttttgaaaaa ttaaagattg catctatcgt
     2641 tcaacaaggc gaacttagtt caatcatcaa agcaaaacca aaagagttca aggaactgtt
     2701 aaacacaata atcggaattg acaagttaga cactgcacta gcattaatga gaactgtaca
     2761 aagggaattt aggacatcta tacaggaaaa atttggttat gatgacacac aaattcagct
     2821 tattgaaaat aaaattactg aatatgaaaa tgaatcagaa aatgcacagc caaaactaga
     2881 acatctgaat acggaaaaga aaaaacaaga atcattgata tcaaaacttg aaaaacaaat
     2941 tcaggatgat tcaacaaagg aatcacagct aaaggatcta gaatcaagaa aaaaggaatg
     3001 gaaagaatat gcaatggata tcataaaatc aatcaaaaaa gaagttgctg aaaaagaaga
     3061 aattgtaaac gaatgtaaac catgttttgg attatctaaa aacaaaaatg aaattgaatt
     3121 ggaaattaat aaaattcaga aaaaaatctt ggataccgag tctgaactaa gtgatttagt
     3181 gaaaaagcaa gttaggcttg aagaacatgg agaacttgct aaaaaattgg tgctgaagga
     3241 tggaaaatgt cctgtatgtg attcatcagt agaacatctc aagccattat tccaaaaaaa
     3301 gcatatagaa gacgaaattc aggaaataga aaaaaaaatt actcaattag aaaatgaaaa
     3361 agatgaactt gaagaaaata ttggcagtct tactggtgag ctagaagaat caaaaaaggc
     3421 tgaaacaatt cttagtacat acaaaattaa gaacgaatca cagttagatg aaattgttgc
     3481 agaaatcaag atgaaagtaa aacaaatgca aaagataccg cttacaatca attctggtca
     3541 attggtagaa actgctagcc tggattctca tgcaaaaact atctatgaga atattgtttc
     3601 acttgaaaaa tcgaccaagg gttttgatca gcaacaattt ctcagaaaca tagaatctag
     3661 agatgtttct gtaagtaaat tgagccagat taatcaggaa tatggagaga tttctggaaa
     3721 tattaaagca acaaagatag aattggaaaa actgagttcc acattagcag aactaaagta
     3781 tgttgatgga tatgttatgg aacttgaacg tattcaggag aatgtttaca atagagatgg
     3841 cccagtaggc aaaagccttc gttcgtgggc attagagata atttcacaaa aggcatcaga
     3901 atatctagag aaacttaaca caaagataca aagaatttcc ttgtcagaaa aaacacgcga
     3961 tgtgaacatt tcgtgttatt ctagaaatac tgtgcttgat cttgaatcac ttagtggtgg
     4021 agaacaggta agtgttgctt tagcattaag acttggtatg tcgcatcttc taggtgcatc
     4081 aaatcttaac tttatgatac ttgacgaacc aactacccac ttagatagtg aacgtagaaa
     4141 atctcttgta aatgttctat ctcaactaac aaatttgaaa gatgatgatt catcaatgca
     4201 attcattatc ataactcatg acgcagaaat ctttgatgat tctgctgtag aaaacatata
     4261 caagtttgaa tcagcacaaa acggaactat agtaagtaga ctttagccac cagtgagttt
     4321 tgcaaatttt tcattcttgc tcaacttttc cataaactct aggttggaca gcgcgacaac
     4381 cgcagagtcc cttactggtt catcaggatc atctaatgcc ttctcaagag tttctcgtgc
     4441 tttcttagaa cccattacac ccaacgcaat tgcagcttca tgtcgtacaa acatgctagg
     4501 atcatttttt gttgcatctt ctaatggttc aataccgtta ctgtaacaca tctgtcctag
     4561 tgaaaatgca gcctcatgtc taacaagttc atttgggtca tctttcagca ccttcgcaat
     4621 atactgcact ttatcctctc caccaatgtc aacaagtatg caggttgccc tacaacgaat
     4681 cacataatct tcatgttcta acagatctac aaaatactca gtttcttttt tttcatattt
     4741 atcttccatt tctgccaaaa gactgagtct tgactgtggc acaacatcca ttacatcaaa
     4801 tttgaataaa ctgggaattt aggtcttctt tagattctga aggtcggaaa tgcccataat
     4861 taccctaaat ctatgtatag acacactcat tgattaacaa tgaagctaat caatgaaata
     4921 tgaggaaaat atagtataga ttaagtgtaa aaatatgtaa aattttataa tttttatatt
     4981 tttcatcgaa aataatcaaa aaattacaaa aatatcatgt tgatattcag tggttacctt
     5041 atatgtcaaa agattactat agtatctatg gcacaaatgc cggcactaat tccaaaagag
     5101 gtagaaattc acagattgaa aaagatctgg cttattgtta tctcaatggg atctactgca
     5161 gcttctgtag aggttgacaa ctttgttgac ggctctctac accagacttc tattagagac
     5221 agcgcattta ctccagcaca ttggtggcta tattctcact ttatagctct gccattggga
     5281 tgggcatctg tagcaatcta tgatagaaaa gttccgattc ttagaggacc aaataattca
     5341 atgaacactg gactcaagat gaccattctt ggttacttgg caacaatgtt tacaattggt
     5401 atcaatgaga tgtggcactt ttggtttgtc gaagaaatct tcgcagtacc aaatcactgg
     5461 atgtttaaca tgggtgtagt cgtagccttt atgggcgctc tagcatacgt agttagggtt
     5521 tatgcacgac tggtcgaact aggtgcagaa acaccaggtg aggacccata tgttgcagaa
     5581 atgtacaaga tggccttaga aggcaaactg tacagcagat caatacctta gaaaaaacgg
     5641 aaaacagaaa agaaggtttt ccgctttctt ttctttttat tttgttaaaa aaattaaaac
     5701 agttatcaaa gttatctttt taggtgcata aaaaaagtga ttgtatggta gtgcaggtag
     5761 gtcagaaggc accgaatcta aagatttctc aatgggttca aggacttgat acgaatttag
     5821 acaaacaggg tgataatata attttggttg atgtgtttca agtaaactgt cctggatgtt
     5881 ttatgtatgg aatacctgaa tctattgaaa tttataacaa gtataaaaca gaaggtgttt
     5941 ctgttttggg tgtagctact gccttcgaag attttgataa aaatacaatc agtaatctag
     6001 aacttttggt gaaaactggt gaagtgattg gtgaaacaaa aaaaatgttg agtcagtatg
     6061 gaaagttaga tggaaataaa ctagcatata aaattccata tcctgtagca atggattcat
     6121 taatcaagga gtctggtgat gtaagcaaag aaaaaatgga ttctatgatt aatactcaaa
     6181 ttcctaattt tgatgcacag tcagaggatt ataaaaatca aatttatgag agagttaaaa
     6241 attactttat gtcaaaggaa tatactgctg agacatttga aatgtattcc cttcagggaa
     6301 ctccttctaa catactaatt gatcgtaagg gggtattacg tgatgtctca tttgggcaga
     6361 atggatcatt agaatcaaaa attcagaatc tggtcaatga ataagtcgtc tttttagaga
     6421 ctacaattat ggagattatg gctaccgcaa atatgagcat cgcaatagta ccaaattcag
     6481 gaactacata actaccataa acttcaatcg tttctgcctc tgatgtgtat ggtattgata
     6541 aaatcttatt ttcaaatttg acattttctg tctgttcccc attaaccaaa acaaagaaac
     6601 ttccatcatt aaacggtcta atcaaaaaat cagatgttgt catagacaat ataccactat
     6661 catctgtctc aagactgaat atgatcgaat tctcacttgt atttgcaatt attcctgtta
     6721 tttcaccact agttaattca gtcattacca tagaagaaag atcaagttct tctggaactg
     6781 atactgttcc gtcaggatgc atgaatccct tgatagtcac atcaccctga taaaaattgg
     6841 gcacagtaaa ataaagatgc caatttccca tttcgtcaag gctttgaagt ctttcaattt
     6901 tttcatgaat aggttgtcct tctgcatatt ttttaccatc tagatatact tcgaggtttt
     6961 catgaataga gctttgagga aattcaaggc ttgcgttcat gtcaagatgc cagctgttca
     7021 atgcatacat attgagagct gtaccttctt ggttgaattg accgtcagta aaccatgcac
     7081 cagaatcata cctgaatgta aacttctcat cgtttctttc tgcattcaat ggaaagattt
     7141 catcgtctcc aaatgcaaag caactgtaaa gatgtatgtt gttagctatt tttggattag
     7201 gtatcatttg aaaatctaaa atctcatctg gatacataac atcaaatttt tgcgaactag
     7261 cgacatctaa tatcacttca tttttatctt tgattaaagc ataaatctta aaatcataaa
     7321 atgtttcatc actggaattt tttatctgac cagtcatatt accatcggaa tgtaaaacta
     7381 gtgagtcatt atacagtacg taaccaccct taaactcata tccagtcaag ttgtatgtta
     7441 ttgcatatgg ttcaagttta ggactatctg atgttatttg tggtagtttg atcttcattg
     7501 ggagcatttg ttgtggcatc actgaagaaa atacataatc atttgaaaaa atattatttc
     7561 cgtcatgaac agtaacagtg attgtcgggg ttacaggata aatttcagta ttttttatac
     7621 cacctatgac agtataaaca ccatcatgat cataaaaccc aacatactcg ttgtcaggaa
     7681 tgtatacttc agcacttaca agtgttggag tgattaaaac aaataatatt attggaatta
     7741 aaaaaaagag tactttcatt ttacgctata tttggtaaaa cacgtcttct catctcttta
     7801 tccttagcaa tacttggatg ggaaggatct gccaatatta tttcaaacca atagtgcata
     7861 ccgtccttgt acaaaaagta ggaaccaagt aatttcatgt taggatatct ttctaaaact
     7921 ctcctctcag atactttcct catagaaaca tctgccttaa ttctagttac acctagatgt
     7981 ttaggtctcc ttccagctct aggtctttgt tttctcattc cgccagtacc aacacgcata
     8041 cggataacaa caattccttg ctttgcctta tagcccaacc ttctagctcg tgtaattctg
     8101 ctgggtttat ctattctagt aatggcattt tcttttctcc aaatgatagc tttttgcctt
     8161 aactcgacag aattgttttt cagcattttt aaccatactt gctcttgact atttggcata
     8221 aagtaggtca aaatgtaccc ttaatattcg taatatcgac aggcaaatta tgaaaatcgt
     8281 cgtattttca ctgtccatct tggtcttatt cataacatta tcaagtttga ccccagctta
     8341 ttctgtacct gtggtaacca ttgaaacgag tcaggagact tatcattatt gtgataaatt
     8401 atcaataaca gtcaatgttt ccgagataac cggtgatgtt gcaactatat acatcatcaa
     8461 ccctgaagaa ggaaaaagta tattgttaag cccaccaatt tctgcagaaa ctacagaact
     8521 tccttcacga tttgcttttg acgcaacaat atgggatcct ggcacatata cactagaaat
     8581 tgaatattca gaaactgctt cctcagtgca gttttctata gatgataacg aagaaatctg
     8641 cctaccatac tggataaagg atgtcgcata catgtggata agcgaaccaa ctgttacaga
     8701 caaagatttt gcgcgtgcac taggatttct gattgagcaa gaaatcatca aaattccata
     8761 cacatctaca ggtgatgaaa cggtaacaaa gatacctgac tgggtaaaaa ctaatgcaga
     8821 atggtggata actgatcaaa tatccgatac cgagtttact ttatcacttc aatatctcat
     8881 agaaaaggga attatcttag taaatacgtc tgcagtgtaa tcttctactt atcttatttt
     8941 ctgtgtgccc ttgctatcaa ggctaaagca actagaactg ccatacctat tccaaatgct
     9001 atagcagttg gaattatcat tgcataaaat gtaccctcct caacttgtgg attgaaagaa
     9061 ccagatttgt caataataca aataccacca tcgtcagttg ttccaggtcc acattgaaga
     9121 gcacccgaaa cttcctctac aatagcatct gcaacatcta ctgcctcatc aataacatca
     9181 acaccggcat caataacatc tccagcctca tcaataattt cagatccagg tgctggaaca
     9241 gagaaagcaa aagtgtatgt ccctttcaat tcaccataat tcaacatgat tacgtaatca
     9301 ccatcaaatt tccacgtagg gccatcagaa ttaatagatg tagagaaagt accatcgtca
     9361 tttggatcaa attgagatac agtaacaata ttgttcttag gatctacaac tctaagtgta
     9421 atttgatata ctttaagtgg gtccgatgta tcaaaatcag aaattaatcc tgatacttct
     9481 atagtcgtac ctttctcata tgatgaggaa tcagttgaca atgttaattt ttcaccatat
     9541 gccataccat agccttggga taaaatcaga acagaaattg caagcaggat tgctatcttc
     9601 atcttagatt acgcccctaa tcgtctgtat tatcttttcg gctataatat ttgcagctaa
     9661 tgactgagct tcttttgtct gggctccaat atgtggagtg gctataaagt taggtagtga
     9721 tatcaattta ttacttgtag caggctctgc ctcaaaaaca tccaatgcag ctccagataa
     9781 atctccattt ttcaatgcgt tgtataatac ttcttcatca atcacaccac ctcgtgaagt
     9841 attgatgata tgagcagatt ttttcatcat agcaatcttt tctgaattta tcatatgatg
     9901 tgtactatca attaatggaa cgtgtagtga aatataatct gaacttgata acaatgtatc
     9961 aagatctgct tgcataagtc caacttcctt tgaaaattct tggtcaattg gaactacatc
    10021 atatcctata atattcatat tcaatgctct ggctaatctt gcgagtcttt ttccaatatt
    10081 tcctaatcca actattccaa gatacttgcc tctaagttca ctacccatca attctttttt
    10141 tatccaattt ccgttacgaa cttctctatc agctcgtgga atctcccttg ccattgaaag
    10201 catgaaacca atcactaatt cagctacagc attcattgca ccttccactg cattaattac
    10261 tttgatgttc ttctcctttg cagcaacttg atcaatatta tcaagtccca ctccgactct
    10321 ggcaattatt ttacagttat ctgctttttc tatcagttct ttaaccagtt ttgttctgct
    10381 tcgtactaca acaacttcat aacttccaat tttttcagat atttgttctg gagtaatttc
    10441 aggttcatag gttacctcaa gaccattttt agtcagaatt tcattaagta tagaatttac
    10501 ttgatcacag attagtactg tatgttttac atccataaat tcagccacta agcacgaaat
    10561 ataatcattt tgagtcacta taacacagtt taggctttaa ttaaaaaaaa agaaaaaaag
    10621 aatgggttta ttctcctgta gctgcagcga taattgctgg aactgctacc catagttcga
    10681 cggcgccttc ggctgcttgt gctgctgcga ctccgtcgct acacatgcag tgaacgttct
    10741 ttgctggatg atggattgat tgtgagccat atggtggtat tgaatcatat ggatttccca
    10801 atactcctgc atacataccg gtatctactg caccttgcac aacacccaag tcagttgctg
    10861 cttggttgat gttaggtagt ggtagaccag caacgaagat ggaggctgct ccagtgtaaa
    10921 ttgctgcata gtcatgatta accgcgacat aaatgcctgg ttcatcataa acgatgtcaa
    10981 caatcatgtt ctgggaacca ccaagtaacc atgtttctgt agcaccagat tgtactctgt
    11041 tgccctgtgt gactctgtca agaatctcac ccacgatgtg gaaataaact ggttcgttgc
    11101 cttggttctc tacaaagatt ctgttatgaa cgccagttgg tgtaaacatc agttgcgatt
    11161 gatattgatg taggtccatg ctattccatg gttgtgctac aaagatgttc tttccagtgt
    11221 caccattgtt aagcaagttg tggatttcgt ttggtacgta accaaatggt tgtccattaa
    11281 ctgctgtgta tgatgtctgg tgtgcaaaca tttttgttgc atcataagta cctgcatcag
    11341 tcaagtacat ctgttggtat tggagtgaaa actccaatgc atcagcagaa tagaattgtc
    11401 tatcccgtga aacatcaccg tttgcattta cagctgtctt ttcaaccatt aacttactgt
    11461 aaccatcaat tggatcaact atggcaatgc catacattcc ttgaaggacg tgttggtcca
    11521 ttgctgctac attaatacca gaacaatggt atttgtatac ccctggtgat tgtgcaatgt
    11581 aacaataggt tttttcagtt cctggctgta ctgcaccaaa tgttggtaca gcagtgactt
    11641 gtgatgcgtg catgtcgtta ccgtgtggag ttggttcacc atctggtaca gttagtgtca
    11701 tctttacgac gtcaccttgt gtaactctta gtgtaggtcc tggtatctgc tcactgaaag
    11761 tcatagcact aaaagtgcca ccacctaatc gtgggagatc aatacttaca ccagtaagtg
    11821 ggaactctac aacagtacga tctgcgtatt catcgctgtc acagtttcca gcctcagcaa
    11881 atgctgctgg cataacaagt tctaatccac ccatgtcatg gatcttctgc aatgctgcat
    11941 tatctgactt tgaaaagtca aatgttgatc ctgtcatttg ggatgtaaaa ctacttccga
    12001 atagcgcgcc gcccataaca gctattgctg ccatggtaaa catgaagttt atgcgcttat
    12061 tcattgtaat caaactgatc atttgccact taatatagct ttttcatgtt tcggctaaaa
    12121 taaaatcatg gttcaatatt tagaattgtt taatgagtat actacatttt gttaaattaa
    12181 tcatttacga aaatatatac taagaataag ctatttgtat atgtgaagaa tcgaagcgat
    12241 aaagattcac ttggtttagt taaaattcct gctgatgcat attatggacc gtttactggt
    12301 agagcagtca aacaatacca tgttactgga caaaagtcgc atatacattt gatcaaatca
    12361 tttgtaatga taaagcgttc tgctgctatt gcaaacatga aagctaaagc attagacaac
    12421 aaacgtggaa gagcggtcgt caaagcatgt gataaaattc ttgcagggaa atatcaggat
    12481 cagtttgtag ttgatatgat aaactctggt gctggtacag cgttcaacat gaacacaaac
    12541 gaagtaattg caaatgttac attaaaaatt cttgggaaaa aattaggtca gtatcaacac
    12601 ttacacccaa atgatcatgt aaacatgtct caatcaagca atgatacata cccaactgca
    12661 atgcatatgt caatactgtt tgcacttgat gacctagtgc caattatcaa aaagctagaa
    12721 acgtctttag ataaaaaagc aaaacagttt gaaaaataca aaaaagttgg acgaacacac
    12781 cttatggatg cattaccagt aactcttgga agtgagttta aggcttattc atcttctgtt
    12841 cgttatgcac gactcaaaat tgaaagcgtc tatgaatcat tgtatagagt tgcattgggt
    12901 ggtaccgccg taggtactgg agcaaataca ccaaatggat atcgtaaaaa tgtaattaat
    12961 gagcttgcaa gaatttcaag tcttccacta aaacctgcgg ctgaaagtca gcatgcactt
    13021 caaagtagac ttgcaattac acatgcatca tctttcttga gaaacctagc tgttgagctt
    13081 ggaaaaattt caaacgatat taggttgatg gcctcaggac ctgttgcggg atttggtgag
    13141 ctcgaaattc ctgcggtaca tgctggctct tcaatcatgc caggaaaagt aaatccatct
    13201 ttagcagaat gcatgaacat gatctgcttt gatataattg gtaatgatac tacagtagct
    13261 tttgctgcac aagcaggaca actagagctt aatgtaatgc ttccagtaat gctcaaggca
    13321 gttcttgatt ctaccgatat gttaacaaac tttttgccaa tatttactac aaaccttatt
    13381 gctggacttg gtgcaaacaa ggaaaaactt caggccaata ttgaaaaaag tcctgttatt
    13441 gtaactctgc tcacaccaaa aattggctat caaaaatcag ctgaactctt caaggaatcg
    13501 atgaaaacgg gtaaaacaat tcgtgaactc gtaatctcaa aaaagttaat gactaaaaag
    13561 caagttgatt ctttactaag ctagaaatgg caaaaatttg ggttgaagcc tatggttgtt
    13621 cagctagttt tgctgattcc gaaatgatat ctggattgat cgtaaatggt ggacatactc
    13681 ttactgaaaa tgaatctgat tcggatctta atctgatcgt cacttgttct gtaaaggatg
    13741 ccactgccac aaagatggtt tatcgtatta aaaaatcaca atcaaaaccg cttattgttg
    13801 ctggttgtct tcctaaggca gaaagacata cagttgaaaa atttgcaaaa aatgccagct
    13861 tgatgggacc aaattcgatt gggaaaactg tgcagattat tgaaaaaaca cttgatggtt
    13921 caaaaatggt gtgcctagat gataccgata tttccaaagt tggtataccc aaagtaagac
    13981 tcaatcatac agtaggaatc gtagaaattg ccagtggttg tatgagtgaa tgtacatttt
    14041 gccaaacaaa actggcaaaa ggtgacttga aaagctacag aatcggagat attgtgagac
    14101 aggtcaaaac agaacttgca gatggttgta aagaaatatg gctctcttca actgataatg
    14161 gttgttatgg cttagatatt ggagaagact tgtcttcttt gatcaacaat gttagtgaaa
    14221 tacctggaga gtttagaatt agagttggga tgttgaatcc tatgtttata cctagaataa
    14281 ggaataatct tctgaaatca tttgaaaatg ataaagtatt tcgattttta catgttccag
    14341 ttcaaagtgg aagtaataat gtactgaata atatgaaacg tggacataca gttgaaacct
    14401 tcaaaaatgt tgtaaaagaa tttagaacaa aatttggtcc atttactatt tcaacggata
    14461 ttattgtagg atatccaacg gaaacagaag aagattttca agaaacagta aaacttttga
    14521 aagaaaccaa accagatatc ataaatcttt cacgatatag cagaagacct ggaactgtgg
    14581 ctgctgaaat gcctcaactt aatgttgcag aagttaaacg aaggagtaaa caaatcaccg
    14641 atattgttaa taatatctca cttgagaata ataaaaaatg gattggatgg aagggcaaag
    14701 ttttgtttga tgaagataca gatggacaga ttaaaggcag gaattttgct tacaaaccaa
    14761 tttttgtaga aaatgatgcc aaacttgacc aaatatccac ggttttgata accaatgcaa
    14821 caaatcacag tctggttgga gagatcgcaa gttaaaaaat tttgatcgtt gttttgatga
    14881 aaacttagtt aacggttatt taccggaatt taccataata tgctaccatg acattcgaga
    14941 taactgaacc tatactggta attggcctag gacgagttgg tgctgatctt gctgaaaagg
    15001 caaaaaaatc cctaaactct aattcgttac ttatcagtca tgaccaaaaa gatttgactg
    15061 gcgaaaactc gataaaaatt tccacaaagt ctgttgttaa cccatcaatt catcttatta
    15121 gaggatcaac tttagaaaac ttggaagaga ttgaaaagaa tatttcaaaa tactctacaa
    15181 taattttaat gtcaaatctt gctggaaagg ctggtatagg aattgctcca gttgtttcta
    15241 aaatttgtaa agaacaagaa aaaaaccttc tatcatttgc tattatgcca tttaaattcg
    15301 aaaaagatag aatattccaa tcaggaattg cgttaaaaag aattagacat gattcacatt
    15361 gtactattgt tattgataat gatgcacttc ttgatagtaa tcctgatctc tctcatgaac
    15421 aatgtaataa tatttccaat aaagccattg aaagtgtcat atcatcacta aaatcatctg
    15481 aaatttctga ggatgtaaat attttatcaa ccagtaaaaa tgctagtgat atggaagtgt
    15541 ctcttaaaga ttcattaaga atgctatatg aagatgcacc tccaaacagc attaaaagat
    15601 ctatgctgta tgtctatggt ggtagtaatg ttccaattgg agtattaaat tcaatttctg
    15661 atattactgg tggagttttt gatgaaaata ctacgcatgt tgacatgtca tctaatgaat
    15721 caaaaatagt catgctgagt tctgttcagg gtgaaacaaa atttgacaga tatgatccac
    15781 ttggaataat ttcttcagag aaaacaattg attgggatga acctgaatgc agtataaact
    15841 gtgaacttga tcttaaacag ttagaataaa taaattagtt tctagaatta cttttttgct
    15901 ttagcagatg gctttgcttt agcagatggc tttgctttag cagatggctt tgctttagca
    15961 gatggctttg ctttagcaga tggctttgct ttagcagatg gctttgct
//
LOCUS       DASCPJ010000012         3048 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000171504, whole genome shotgun sequence.
ACCESSION   DASCPJ010000012 DASCPJ010000000
VERSION     DASCPJ010000012.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000171504"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="QGF01_00645"
     CDS             <1..434
                     /locus_tag="QGF01_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19446.1"
                     /translation="GNMFSVVLFPHRWIFENQEAWHTGNGVGFGSDIEDAKGIDHPPV
                     TAGAFFAAKLGVAEYLLEKKCQASALVLREIRPEYAVPVGVWQIREAIRSAMKNELYI
                     AENFDDGITFAAKRMSVSKSEWLSNGNMLSMLKQKSISDFF"
     gene            486..1418
                     /gene="artG"
                     /locus_tag="QGF01_00650"
     CDS             486..1418
                     /gene="artG"
                     /locus_tag="QGF01_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumarchaeosortase"
                     /protein_id="HJN19447.1"
                     /translation="MHRNTILAILLISSPILFVLAAYPDTFSMSWNQGRGGFLFALAF
                     IVAELVGIKFAVSRNNLIFAIPLAVITITYFVALNFGLHDYIMDAAPAFDVHLIYSWE
                     WFWDFVVITIFVTVSAAILFGKKWIRILPAGPIFLGGSAIILSLDAFFPYDTLGPLQY
                     FVPYLVQTNVWIINIFDLGIATARDNLMFLQGEHGPFALQVFWPSAGVHSVIIYSLVM
                     MAFLLKMNIQRKRKALYFGLGIIGTIIINLIRIFSLSLFALKVSTNPVEFEEYHSVAG
                     EIMFLPWLFVFLLVVTFIESRRLKKKEISTKSNL"
     gene            complement(1402..2496)
                     /gene="dinB"
                     /locus_tag="QGF01_00655"
     CDS             complement(1402..2496)
                     /gene="dinB"
                     /locus_tag="QGF01_00655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HJN19448.1"
                     /translation="MKQRIILHIDFDYFYAQCEEVRKPELKTSPVVVCMFSDRGNDSG
                     AVATANYIAREYGVNSGLSIKFAKKRLEERTDSVFLPADFEFYSSVSEKSMNIIKEFA
                     DVFEYVGRDEAYLDVSKKIEHDFKRASHISQQIKNKIREETKLTCSVGVSGNKLLSKI
                     ASNYKKPDGLTVVLPDRISQFIEDLEIRDIHGIGGKTEERLAEEGIKTISNLKDLDIF
                     SLVQMFGRKSGTYIFNAVRGIDDEPVTPRAPTAQIGKIVTLKKDSDDYDFLEEALLQL
                     CDNLNAVIIKENKAFKIVGIQLTQIDLSNKTKSRMLKNPTSSLEMLQKTSKQLLREML
                     DDKPDLVRRLGVKVSDLTTIEGQSNITNYF"
     gene            2574..2822
                     /locus_tag="QGF01_00660"
     CDS             2574..2822
                     /locus_tag="QGF01_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19449.1"
                     /translation="MTYPISAEKDGVKIKPELMEKEKLYHCIFNDKVVLLFKDQNEIL
                     NCYEIEDEQIVSKVKSSKEEDIEEILEEYISKENLKIK"
BASE COUNT          974 a          528 c          507 g         1039 t
ORIGIN      
        1 ttggaaacat gttttcagtt gttttgttcc cgcatcgctg gatttttgaa aatcaggaag
       61 catggcatac tggtaacggt gttggttttg gttctgatat tgaggatgca aaaggaatag
      121 accatccacc tgttacagcg ggtgcatttt ttgcagcaaa acttggcgtt gctgaatatt
      181 tacttgaaaa aaaatgtcaa gcatctgcac ttgtattacg tgagattaga cccgagtatg
      241 cagttcctgt cggtgtttgg caaatcagag aagcaatacg ctcagctatg aaaaatgagc
      301 tgtatattgc agaaaacttt gatgatggta ttacctttgc agcaaaaaga atgagtgtta
      361 gcaagtccga gtggctttct aatggtaata tgttgagtat gttgaagcaa aaatcaatct
      421 cagatttttt ttagaattga gtccgtatct atagcgtatt tattgaactt ttttactttg
      481 atagtatgca tagaaataca attcttgcaa tattgcttat ttcatctcca attctattcg
      541 tactagctgc atatccagac acattcagta tgagctggaa tcaaggacgt ggcggatttt
      601 tgtttgctct ggcattcatt gtagctgaat tagtgggaat aaaatttgca gtttcaagaa
      661 ataatctcat ttttgcaatt ccattggctg taataacaat aacttacttt gtagcgttaa
      721 actttggatt acatgattac ataatggatg cagctcccgc atttgacgta catttaatct
      781 attcatggga atggttttgg gattttgttg ttatcacaat atttgttaca gtcagcgccg
      841 caattctgtt tggcaaaaaa tggattagaa ttcttcccgc cggtccaatt tttcttggtg
      901 gaagcgcaat aattctatca ctagatgcat ttttcccgta tgatacactc ggtccactac
      961 aatactttgt accttatttg gtacaaacaa acgtatggat aatcaatatc tttgatcttg
     1021 gaattgcaac tgcaagagac aatcttatgt tcttacaggg tgagcatggc ccatttgcat
     1081 tacaagtgtt ctggccatca gccggtgtac acagtgtaat catctactca cttgttatga
     1141 tggcattttt attgaaaatg aacattcaga gaaagagaaa ggctctatat ttcggtcttg
     1201 gaataattgg tacaatcatt ataaatttga ttagaatatt ctcactatca ttatttgcat
     1261 tgaaagtgtc aacaaatcca gtagaatttg aggaatatca ttctgttgct ggtgaaataa
     1321 tgtttttgcc gtggttgttt gtatttttgc ttgtagtaac tttcatagaa tctagacgtc
     1381 tgaagaaaaa agaaatttca actaaaagta atttgtaata ttactctgtc cctcaatagt
     1441 tgtaagatca ctgactttca ctcctaacct tctcaccaaa tctggtttat catcaagcat
     1501 ttcacgaagc aattgcttgg aagttttttg taacatctcc agactagacg tcggattttt
     1561 taacattctt gattttgttt tgttagataa atcaatttgt gttaactgta ttccaacaat
     1621 tttaaacgcc ttgttttctt tgataattac tgcgtttaga ttatcacaca actgaagcaa
     1681 tgcttcttct aagaaatcat aatcatccga atcttttttg agagttacaa tcttaccaat
     1741 ttgagcagta ggtgccctag gcgttacagg ttcatcatca atgcccctta ctgcattgaa
     1801 tatgtatgtg ccagattttc taccaaacat ttgaactaaa gagaagatat ccaaatcttt
     1861 tagatttgag attgttttga taccctcctc agccaatctt tcttcagttt tccccccaat
     1921 accatgaata tccctaattt ctaaatcctc aataaattga gagatcctat caggtagtac
     1981 aactgttaag ccatctggtt ttttgtaatt tgatgcaatt tttgatagta atttatttcc
     2041 agatacgcca acagaacatg ttaattttgt ttcttctcga attttatttt ttatttgctg
     2101 agaaatatgg ctggctcttt tgaaatcatg ttctattttt tttgatacat ctagataagc
     2161 ttcatctcta cctacatatt caaacacatc agcaaattct ttaattatat tcatagattt
     2221 ttcagataca ctagaataaa attcaaagtc tgcaggcaaa aacacggaat ctgttctttc
     2281 ttctaatctt ttctttgcaa attttattga taaaccagaa tttacaccat attctcttgc
     2341 aatataattt gctgttgcga ccgctccact atcattacct ctatctgaaa acatacatac
     2401 cactacagga cttgtcttta attcaggttt acgaacctct tcacactgcg cataaaaata
     2461 atcaaaatca atatgaagaa ttatccgttg cttcattttg ttactttaac ataaaaccat
     2521 attaccatat ctataaggaa attatctaaa tatcatcata atttgatata attatgacat
     2581 atcctatttc agctgaaaaa gatggtgtaa agattaaacc tgaattaatg gagaaggaga
     2641 agctatatca ttgtatattc aatgataaag ttgtattatt attcaaggat caaaatgaaa
     2701 ttttgaactg ttatgagatt gaggatgagc aaattgtttc aaaagttaag tcatctaaag
     2761 aagaggatat tgaagaaata ttagaagaat acatctcaaa agaaaacttg aaaataaaat
     2821 agctctatga tccttaaaga taaaataaaa ccacatgcat ttatgtatta gatacgtgat
     2881 gggggcagaa aataaaaaaa gcgatgcaga agaaattctt tccgaattta ataaaagaca
     2941 tggaattcca gattcagaac cagaagcacc ctcagaacca gaagcaccct cagaaccaga
     3001 agcaccctca gaaccagaag caccctcaga accagaagca ccctcaga
//
LOCUS       DASCPJ010000013        16409 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000186245, whole genome shotgun sequence.
ACCESSION   DASCPJ010000013 DASCPJ010000000
VERSION     DASCPJ010000013.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 16409)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16409)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16409
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000186245"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="QGF01_00665"
     CDS             <1..1141
                     /locus_tag="QGF01_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HJN19450.1"
                     /translation="RVFDGLGNPKDNGPKIVSKEKFDLVGSSINPYSREEPAEFIQTG
                     MSNIDGMNTLVRGQKLPIFSASGLPHNQLASQIARQAQVLGTEENFSVVFAAIGITSE
                     EANFFIKQFEESGSLERTALFLNLSSDPSMERILTPRLALTTAEFLAYEREQHVLVIM
                     TDMTNYCEALREISAAREEVPGRRGYPGYLYTDLASIYERAGKIKGRKGSVTQLPILT
                     MPADDITHPIPDLTGYITEGQIVMNRELHRANVQPPVDVLTSLSRLMNQGIGEGRTRE
                     DHRQLADQLYAAYAQGKDARALAAIVGEGALSDLDRKFLGVANNFEKKFVHQGPNENR
                     SIEQTLSLGWELLAGLSETELTRIKPEFIAKYGKKINTGDTDKSE"
     gene            1152..1811
                     /locus_tag="QGF01_00670"
     CDS             1152..1811
                     /locus_tag="QGF01_00670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HJN19451.1"
                     /translation="MPSNADIRPTRLEYLRTKKRIKIAQKGLKLLKLKRQALILEFFS
                     ISKASSRLRGELNAELVKGYQSIRMAEMIDGEMRLENEAMKIPQLKKLSIIPKNVMGV
                     KIPRLEGGRREEALTDYLLEIPVSISEAMKAFQEVHKIVLDVAEKETTLRKLLYEIDK
                     TKRKANAIENVFIPRLESAVKFITFRLEEMERDTFAMLKTVKRKMNEREENKEVELIA
                     N"
     gene            1931..2260
                     /locus_tag="QGF01_00675"
     CDS             1931..2260
                     /locus_tag="QGF01_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19452.1"
                     /translation="MSASQDQYINSLKKIKQVEEDAQKEIDSYKSKIEDQIIQLDSEL
                     EKAIAAAKSEGEKLVDSNVEKATKKANAETQKTIEEAKTKSKDITSQITAQQAQDIIN
                     ILLKGVE"
     gene            2262..4373
                     /locus_tag="QGF01_00680"
     CDS             2262..4373
                     /locus_tag="QGF01_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HJN19453.1"
                     /translation="MFKPVKMAKIAIMGLRKNQQTTVSILHDMEILQLEPLSKDVSAI
                     LKNERDNELTRQVSDQLLRVKALMTVLPSIPLTSCRRFESINDLLHTAISIDIDEQVA
                     SLEKEKEALLTEIQETENNLKLVEEFSFFPEDLKILQLSSATSYFGRIPSDKFEEFKK
                     VIDEHQKDIMLYTKAEKELTHLILVVFPTISPDKFANIIQLHNVKIEAVPSISGKPNE
                     IITNQKSNLQTQNQRLEQINNKLNQISEKHFADLVEVEEQLQIENKKLEVISNLGVTK
                     DAFAMEGWIPKSKLGQVESTLEKFTDGTTIYELEAKEEEKAPTLMSNPPRFRLFEAFI
                     RFYSLPQSKEFDPTMVFALIFPIFYGLMIGDTGYCIVILLVCLWVIRRVEKGKRNLNI
                     MPRQLRSFAMLILKKRQMVKLSKAMIPGCVVGIVLGFIFDLHFGFHMNGYLFDVLASA
                     GVTGLPEPGEILNRPSQAFLDPIHNAGTLLLYAGYIGIGFVSFGLILGVIDCMREGEK
                     KEALVKVGWLMVGWGIVLLGLALIGGDAINPTWDRLVEVNPIAYMYYGLIFGGIALMV
                     ACDKSKGPMKVMALMEVATIISHILSYTRLIGILLASVILAHTIDYVFLKSINIGIPL
                     VILGTMILFIGHLFNIIIGVFEPGIQGARLVYVEYFSKFYRGNGRAFKPFGSLRRFTE
                     NQFKSEQPEKEKSVKPKLKVK"
     gene            complement(4568..5029)
                     /gene="pyrI"
                     /locus_tag="QGF01_00685"
     CDS             complement(4568..5029)
                     /gene="pyrI"
                     /locus_tag="QGF01_00685"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HJN19454.1"
                     /translation="MDQSDLTVRRIKDGTVIDHINVGSGLKVLKALQIDGSDGNVITI
                     ALNVPSGKLNKKDIIKVEGKFLEDDDTNKLAVIAPTSTVNIVKDYKLVEKRRVSLPKE
                     IDRIFRCSNPECVTNSNEHIESIMDVLDKEKLVLKCRYCNRVLDVNQLKYS"
     gene            5070..5999
                     /gene="pyrB"
                     /locus_tag="QGF01_00690"
     CDS             5070..5999
                     /gene="pyrB"
                     /locus_tag="QGF01_00690"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HJN19455.1"
                     /translation="MNDFFQKDIISIRDLGKENLESIFSATEKIIDLESNQRREIARG
                     KTLGYLFFEPSTRTRLSFQSAMALIGGTSLGIADVSSSSVQKGESLSDTVKVMSSYTD
                     VLVLRHTLDGSSRFAAEISQKPIINGGSGTEEHPTQAIQDLFTIKREKKNIDGLKIGI
                     VGDLKYGRTVYSLLYGLGNYNVEVHLISPEALRIRSDSAYDIKKQLSYTESTNLDDYI
                     DDLDVLYVTRIQKERFPDEEEYTKVKGSYVIGPDMLKKMKDDAIILHPLPRIDEISPD
                     IDDAKQAKYFEQAEYGKYTRAALLGLILNENGF"
     gene            complement(6007..7497)
                     /locus_tag="QGF01_00695"
     CDS             complement(6007..7497)
                     /locus_tag="QGF01_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HJN19456.1"
                     /translation="MNSRKLALILIGLMLIPISQAFGHGLGIDTTTSLNYEERKILVS
                     VEMLPFYFDESENKKIRIYAFDKKTEENISNVTFLIDIYKNNKLILHDSFFAPDGELI
                     IDVKEYDYLEPYFKSGGLYHYEIDITSIDEVDNVTDSLSGYIADVSVVDTTYHKFKNS
                     ENIDVEFRHKSYYDEISNFVYEPLEKIITFEMPFNWETKNISHLQVVHEEIFFPKNFT
                     EMISPSYKAQINGIDVFKSNLQIDDYSDEDGRIIHFILLQDHVKFFKNTIKQQQAEHG
                     IDTFPDKIYFQLVPNDELIFPVSVLTVNGKYQIDLSWVPKEIMSNSETKFVYTVRNGE
                     NLELEYNSNFDFVVSKDGEQIHRSAEQTRIGGGAIDFVFPSDGQYTIIFDKIRNSESF
                     TSFTLIVGQPTENKTSIPDWIKNNAGWWAEGQIDDSAFLQGIQFLINEGIMIIPSTET
                     TQAPESQEVPSWIKNNAGWWAEGQIDDNTFVSGIQYLVKVGIIILG"
     gene            7682..8719
                     /locus_tag="QGF01_00700"
     CDS             7682..8719
                     /locus_tag="QGF01_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HJN19457.1"
                     /translation="MAIVVAIVSFGFVGYLVFPDVYITAQEQPEVFVTHTGAVVQTTG
                     DVLDPVYTIQEVEFDPEKYLRDFDYGKVSQLEDGTILREYTIVAEDDKIMEIAPGIFY
                     NVWTFNGQVPGPTIRATEGDLVKIYFTNNGSKEHTIHFHGNHPAEMDGIFEIVGSGGG
                     QFTYEFIAGPAGVHPYHCHVMPVEEHIGHGLYGVFIVDPKEGRQQADEMVLMLNGFDT
                     DFDGENNLYAANTIPFYYHHHPIQVNTNELIRVYVVNMVGLDPINNFHLHGTLYKYYP
                     TGTDMIPSEFTDMTTFSQSERGILEFEYQDTGRYMFHAHLTEFSEKGWSGVFYAKDSS
                     QLNMEGYAYGS"
     gene            8709..9881
                     /locus_tag="QGF01_00705"
     CDS             8709..9881
                     /locus_tag="QGF01_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation transporter"
                     /protein_id="HJN19458.1"
                     /translation="MEVKRSSNVKTAVSVIIPFVLLAIMIGYVFGPGSELIGYGVVLP
                     EISIEKVEFVDSEIIATVRNTGQISVNIVMADINDRIYPAAIEPDKHLERFESAIVRI
                     PFEWNEGEPYAVGLTVDDGTRFEKQVDVAVQSIKPTVEMISYFAVIGTYVGIIPVLIG
                     LLWFPFISKLSRSKYKFFLALTVGLLLFLGISTAEEAIEISANNLSDVFNGVLLVATV
                     AVVSFLALNYVGEKLRKRAGASKLAGPVAIALMIAIGIGLHNFGEGLAIGAAIVLGEA
                     ALGAFLIVGFALHNTTEGFAIAAPMARTKLMIGKLAAMGMIAGVPAIFGAWVGGFSYS
                     PLAAVIFLAIGTGAIFQVIVSIMRWIQNEEGKLSNSSVLAGIAVGMIIMYITSILI"
     gene            complement(9891..10379)
                     /locus_tag="QGF01_00710"
     CDS             complement(9891..10379)
                     /locus_tag="QGF01_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19459.1"
                     /translation="MLKLAIKDILKQKSFGGIAIVSGFLLGLIYYFLTLSSAVNHNLI
                     ALVVSPQYLAASIGLTIIVAILGGINIALVVYNIKTQRMNLQKGGSSAIFGGALAAFT
                     PGCPACTTSLTAVLGIVGGLAIFPLQGLELKFVSIAALSFSIWWAMRNINKANCCSMK
                     SN"
     gene            complement(10420..11586)
                     /locus_tag="QGF01_00715"
     CDS             complement(10420..11586)
                     /locus_tag="QGF01_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJN19460.1"
                     /translation="MQNSRLSNNAQVSIIIPTYNESKNIIQILKSIGEHLPKNLLAEA
                     IVVDDNSPDGTGKIVESYLNEMKQKVGYTIDVIHRKAKNGLSSAILNGIQHSSGETII
                     VMDSDFSHPPKIIPRLIEEIKKSRCDIAIASRYMTGGSIKGWTSKRKLLSKTATKIAK
                     TGLGVNQSDPMSGFFAFRRKIIDGLKFDAIGYKILLEILVKAKGVKVKEIPYTFTNRV
                     HGSSKLDSSTMIDYARSVWKLYRYGRKAENSETRNSVRFISKASRFYTVGASGLLINY
                     LASLMFLDSTMNFWYIHATTVGIIISMSSNFILNKIWTFGDRSFEARKTVIQYGKFVG
                     FSSLGGLVQLGMVFVLVDSYQFTYPMALVFAVAIAASSNFILNKKWTFKEKIWN"
     gene            11813..12736
                     /locus_tag="QGF01_00720"
     CDS             11813..12736
                     /locus_tag="QGF01_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HJN19461.1"
                     /translation="MKVITLSSLVVLFAIASMVAIAPNAFADHHSATVTNAPGSSVPG
                     CEETADGCFIPNTVTIDIGGIVTWENNDTAAHTSTGGSATDGPSGVFDSSLIMAGSSF
                     SHTFEDAGTFDYFCMVHPWMVGTVIVEDMAAAAAAEADAAEAAAAEADAAEAAAAEAA
                     AAEADAAEAAAAAMTVRDPAVDLVDTLSHSTSNGSVSSIMTNSDDATLVVAMDTSDDG
                     ELSINLDSDYITAFDDGSYFVLVNGEEAAFSQDGNDLTIPFEAGTEKIEIVGSVVVPE
                     FGTIAMIVLAVAIVSIIAITAKTRTTLIPKL"
     gene            complement(12756..13391)
                     /locus_tag="QGF01_00725"
     CDS             complement(12756..13391)
                     /locus_tag="QGF01_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19462.1"
                     /translation="MKYAFFAIMGLVVLGFGFTFAYAHTTIQVDPYEIEVGWQDEPPV
                     VGILNAITIDIREPGDVEGVSTGVINAFKKLDASVVSGGASKVLDINTDPRPGHYYAK
                     IIPTKTGSLEVKLAGEINGVQINTIIPIEDVESTSILDFPPTSTSSSGQEVTALKNAV
                     TSLQQDISSIKAQSSTSTGSDEGAAYNFAVFGISLGAAGVILAIIAMVKRK"
     gene            13491..16295
                     /locus_tag="QGF01_00730"
     CDS             13491..16295
                     /locus_tag="QGF01_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HJN19463.1"
                     /translation="MNKLLFLPFLVLIIGIPSAYAHPFLLDSDPGQGQNAAIGTTQII
                     TFYSEAIEIDFSELKVFDSNGNKIDNMDTSYYDGENSLVITTPPLEDGIYTVTSKVLS
                     KIDGHLVQAAIIFGVGEVEVDLSLLESQEESETTFLPEAAARFPGMVGQTIVLGSAIA
                     AIAIWGTQLKHFGKESRVLLNQVYRSKFAKITGISLLAVFTSNFIMLGIQTMRLETSP
                     MSVIDTTFGTTWLIRMIITSILLGIWFWMERKQHLSTKKHIPMLIASLMLIFTTTMMG
                     HGASTELSAPIILDYVHNLLASVWIGGVIFFGFVLLPTLASIDGTQKSKAIIAILPRY
                     SGMIIIALGILIITGPLLLWFLESDVSSLTNSTYGSLIIAKIMLAIVMIGFGAFYQFK
                     IQKPAERNLKSGNMFTFNKLSKLLKAEAMVGIVLLGVVALLVNSSLPAGEIQTVDAQD
                     ITFGFSSNLFSEKAEFDVLLMPASVGQNTISVLVSNVSGEPLSDISNLKIKVSNPQRN
                     IAPIEVPMIEQKLDNETRFEGDITFGFAGIWQIEIEAQRIQTVNEGIIFDILVKPTLS
                     EIRTEITEYDFPDPDSAPLYPRFDGDNTIWISDAAKPQLWEFTLDDQQFKSHEFNGLT
                     TIFIDIDQNGKVWFTDTPNSMIGSFDPKTEKFETIEIPILDIISEQSIPIAVKADFED
                     RIWVALVDKNKLLAYNQNTNNFDKFLTIETEESGPSALLVDDGGNVWFAEALAGKIGV
                     VDSKTFEITEFVPDAPLNEPFALLFDKSGSLWISEHTGPGITKFDPILETFERVKAPN
                     PESLPFGLAIDKYDNIWFGQHITDELAVYDPYNDQLIEVSIPTPESFTQFTTSDNDGN
                     IWFVEQRGNKLGMVSISSVPSQAKAVIVGNDEFNVNYTEIVSPLIAGGIIISSLFFVK
                     SVHDKRRIDGLLQNDITKPS"
BASE COUNT         5418 a         2823 c         3040 g         5128 t
ORIGIN      
        1 aagagtcttt gacggactag gaaatccaaa agataacgga ccaaagattg tatcaaaaga
       61 aaaatttgat ctagtaggtt ccagcattaa tccatattcc agagaagaac cagcagaatt
      121 cattcaaaca ggaatgtcaa atattgacgg aatgaacaca ctggtaaggg gtcaaaaatt
      181 accaatcttc tcagcatccg gcctaccaca taatcaactt gcatcacaaa ttgcaagaca
      241 ggcacaagtt cttggcacag aagaaaactt ttctgttgtc tttgcagcaa ttggaattac
      301 aagtgaggag gcaaactttt tcattaaaca atttgaagag agtggttcac ttgaaagaac
      361 tgcattattc cttaacttat cttctgatcc ttcaatggaa cgtatactta ctcccagact
      421 tgcattaacc acagcagaat ttcttgcata tgaacgcgaa caacatgtct tagttattat
      481 gactgatatg acaaactatt gtgaagcatt aagagaaatt tctgcagcac gtgaagaggt
      541 tccaggaaga agaggttatc caggttattt gtatacagat ttagcttcaa tctacgagcg
      601 ggcaggaaaa atcaagggaa ggaaaggttc agtaacacaa ctaccaattc ttacaatgcc
      661 tgctgacgat atcacacatc caattcctga tcttactggt tacatcactg agggtcagat
      721 tgtaatgaat cgagaattac acagagcaaa tgttcaacca cctgttgatg ttctaacaag
      781 cctgtcacgt ttaatgaacc aaggaattgg tgaaggaaga acaagagaag atcatagaca
      841 acttgcagat caactttatg ctgcttatgc acaaggtaaa gatgcaagag cacttgctgc
      901 tattgtaggt gaaggcgcat taagtgatct tgatagaaaa ttccttggag ttgcaaacaa
      961 ttttgagaaa aaatttgttc atcagggacc taacgagaac cgttctattg aacaaacact
     1021 ttcacttggt tgggaactat tagcaggact ttcagagact gaacttacac gtatcaaacc
     1081 agaatttatt gctaaatatg gaaagaagat taacaccggt gatacagaca aatctgaata
     1141 ggtgatttga attgccatct aacgctgata tccgtccaac ccgtcttgaa tacttacgta
     1201 caaaaaaacg aattaagatt gcacaaaaag gacttaagct gttaaaacta aaacgccaag
     1261 cattaattct cgaatttttt agtattagca aagcatcatc tagattacgg ggagaactaa
     1321 atgcagaact agtcaagggt tatcaaagca ttaggatggc agagatgatt gacggtgaga
     1381 tgagattgga aaatgaagca atgaagatcc cacaactgaa aaaattatcg atcataccaa
     1441 agaacgttat gggagtaaag attccaagat tagaaggagg acgaagggaa gaggctctta
     1501 cagattattt attagaaatt cctgtttcca taagtgaagc aatgaaagca ttccaagaag
     1561 tgcataaaat tgttctagat gtagcagaaa aggagactac attacgaaaa ttactttatg
     1621 aaattgacaa gacaaaacga aaagctaacg caatcgaaaa cgtctttatt ccaagattag
     1681 agtcagctgt taaatttatc acattcaggc ttgaagagat ggagcgggat acgtttgcta
     1741 tgttaaagac tgtaaaacga aagatgaatg aaagagaaga gaataaggag gtagaattaa
     1801 ttgcaaatta attttaaaaa ttctcagcaa aaaaaatttt attatattaa acgcgcaatg
     1861 atgatgggaa atatcttcgt agctgttgct gttatattgt atttgagtaa ggagtttttg
     1921 ggtgcataat atgtcagcgt cacaagatca atacattaat tcattgaaga agatcaaaca
     1981 agtagaagag gatgctcaaa aagaaattga tagctacaaa agtaagattg aggatcaaat
     2041 cattcaatta gactcagaat tagaaaaagc aatagcagca gcaaaatctg agggtgaaaa
     2101 actagttgat tcaaatgtag aaaaagctac caagaaagcc aatgctgaaa cacaaaagac
     2161 gattgaagaa gcaaaaacaa aatctaaaga tataacatca caaattacgg cacagcaagc
     2221 acaagatatt atcaatatat tactaaaagg agtagagtag aatgttcaaa cctgttaaaa
     2281 tggctaagat agctattatg ggtttacgaa aaaatcaaca aactacagtt tctattttgc
     2341 atgatatgga aattttacaa ttagaaccat tatcaaaaga tgtctctgcc attttaaaga
     2401 acgagcgtga taatgagcta acaagacagg tatctgacca attattacgt gttaaagcat
     2461 tgatgactgt gttacctagc atacctctaa cttcctgtag gcgttttgag tctatcaatg
     2521 atctattaca caccgcaatc tcaattgata tagatgagca ggttgcaagc cttgagaagg
     2581 aaaaagaagc gcttctaact gaaatccagg agactgagaa caacctaaaa cttgttgaag
     2641 agtttagctt ctttccagaa gacctgaaga tccttcaact ttcctctgca acttcctatt
     2701 tcggtagaat tccatccgac aaatttgagg aatttaagaa agtaattgat gaacaccaaa
     2761 aagatatcat gctttacact aaagcagaaa aggaactaac acatctaatt ctagtagtat
     2821 tccctactat ttcacctgat aagtttgcca acattataca actacataat gtcaagatag
     2881 aagccgtacc cagcataagt ggaaaaccaa atgaaatcat cacaaatcaa aaaagtaatt
     2941 tacaaacaca aaatcaacga cttgaacaga tcaataataa gctaaaccag atttcagaaa
     3001 aacactttgc agatttagtt gaagttgagg aacaattaca gatagagaat aaaaaacttg
     3061 aagtgatttc taatcttggt gtcacaaaag atgcctttgc aatggaagga tggattccaa
     3121 aatcaaaact tggtcaagtc gagtctactt tggaaaaatt tacagacggt acaacaatct
     3181 atgaactaga agctaaggaa gaagagaaag caccaacatt gatgagtaat ccacctcgat
     3241 ttagattatt tgaggcattc attcgattct attccttacc acaaagcaaa gagtttgatc
     3301 ctaccatggt ttttgctctc atcttcccaa tcttttatgg attaatgatc ggtgatacgg
     3361 gatattgtat agttatcttg cttgtttgtc tatgggttat cagacgagtt gagaaaggaa
     3421 aacgcaatct aaacataatg cctaggcaac tgcgtagttt tgcaatgtta atattgaaaa
     3481 aacgacagat ggtaaagcta tcaaaagcaa tgattcctgg ctgtgtcgtt ggaatagttt
     3541 taggatttat ctttgattta catttcggat tccacatgaa cggttatctg tttgatgttc
     3601 tagcaagtgc aggggttact ggacttccag aacctggaga aatcttgaat cgaccttcac
     3661 aagcatttct agatccaatt cataatgcgg gaacactctt gctttatgca ggatacattg
     3721 gaattggatt tgtatcattt ggcttgattt taggtgtaat agattgtatg agagaaggtg
     3781 aaaagaaaga ggcattagtc aaagttggct ggttaatggt tggttgggga atagttcttc
     3841 tcggattagc tctaataggt ggtgacgcta tcaacccgac atgggataga ctggttgaag
     3901 ttaatccaat agcatacatg tattatggat taatcttcgg aggtatcgcg ttaatggttg
     3961 catgtgataa gagtaaagga cctatgaagg tcatggcatt aatggaagtg gcaacaatca
     4021 tcagtcacat tctttcctat acaagactga ttggaatttt actagcatct gttatacttg
     4081 cccatacaat cgactacgtt ttcttaaaat cgattaacat aggaattcca ttagttattc
     4141 ttggtacaat gatattgttc atagggcatt tattcaacat aatcattggt gtctttgaac
     4201 ccggaattca aggtgcaaga ttggtttacg ttgagtactt ctccaagttt taccgtggta
     4261 atggtagggc attcaagcca tttggatcat tacgaagatt tacagaaaac caattcaagt
     4321 ctgaacagcc agaaaaggaa aaatcagtta aaccaaagct aaaggttaaa tagaaaattt
     4381 acaaaatctg attaaaattt tacaaagaag tatcacaaaa ttaactagtt tgatcattat
     4441 ccaaccttgt aagtcaaata tgcaaagaat gggattctca cgatgctccc ctatacgtgt
     4501 ggattccatc tttttgcatt aaaagacaaa caggactatg attatttgtt tattattgaa
     4561 aattaagtta agagtatttt aattggttta catctaatac tctgttacaa tatctacatt
     4621 tcaaaactag tttttctttg tccaagacat ccatgattga ttcaatatgc tcattactgt
     4681 ttgtaacaca ctcaggattc gagcatctaa aaattctatc aatctccttt ggaagcgata
     4741 cgcgtcgttt ctctaccaac ttgtaatctt tgacgatgtt aaccgttgat gtcggtgcta
     4801 tcacagcaag tttatttgtg tcatcatctt caaggaattt tccttccacc ttaatgatgt
     4861 cttttttgtt tagttttcca ctaggaacat ttagtgctat tgtgataacg tttccatcac
     4921 taccatcaat ctgaagtgcc tttaacacct taagcccact tcccacattg atatggtcta
     4981 tcacagtgcc atctttgata cgtctgactg tgagatccga ctgatccatt agtatagttt
     5041 gtatatatgc ctctataaat ggttcaaaga tgaatgactt tttccagaag gacattatct
     5101 caattagaga tcttggtaag gaaaatctag aaagtatttt ttctgctact gaaaaaatta
     5161 tagatttaga atctaatcaa agacgcgaaa ttgcaagagg taaaacactt ggttaccttt
     5221 tctttgagcc aagcacaaga actcgcctaa gcttccaatc tgctatggca ctaattggtg
     5281 gaacttctct tggaattgca gacgtatctt catcatcagt acaaaagggt gaaagcttat
     5341 ctgatacagt taaggtcatg tcatcctata cagacgttct tgttttacga catacgcttg
     5401 acggttctag taggtttgca gcagagattt cacaaaaacc aataattaat ggtggaagcg
     5461 gaactgaaga gcatccaaca caagcaattc aggatctatt cacaatcaag agagaaaaaa
     5521 agaatattga tggtctgaag attggaatag ttggagatct aaaatatgga agaacagttt
     5581 actctctttt gtatggacta ggtaattata atgtagaagt gcatcttatc tccccagaag
     5641 cacttagaat tcgctctgac tctgcttatg atatcaaaaa acaattatca tacacggaat
     5701 ccacaaatct agatgattac attgacgatt tggatgtact atatgtaact agaatccaaa
     5761 aagaaagatt tcctgatgaa gaagaatata ctaaggtaaa aggtagttat gtgattggtc
     5821 cagatatgct aaagaaaatg aaagatgatg caatcatatt acatccacta ccacgtattg
     5881 atgaaatttc tccagacatc gatgatgcca agcaagcaaa atactttgag caggcagagt
     5941 atggaaaata tacacgagct gcattactcg gtttgattct caatgaaaat ggtttctgaa
     6001 cttgaatcag ccaagaataa taattccaac tttgactaaa tactgaattc ctgaaacgaa
     6061 agtattgtca tcaatctgtc cttcagccca ccatcctgcg ttatttttga tccagcttgg
     6121 aacttcctgt gattcagggg cttgtgttgt ttcagtagaa ggtataatca ttattccttc
     6181 attgatcaga aattgtattc cttgaagaaa cgctgaatca tcaatctgtc cttcagccca
     6241 ccatcctgcg ttatttttga tccagtcagg tattgacgtt ttattctcag ttggttggcc
     6301 aacaataaga gtgaatgaag taaaagattc actatttcta attttatcaa aaattatagt
     6361 atattgacca tcagacggaa aaacaaaatc tattgcccca ccaccaattc ttgtttgctc
     6421 agcacttctg tgaatctgtt caccatcttt agatacaaca aaatcaaaat tggagttata
     6481 ttctaactca agattttcac catttctaac tgtataaaca aattttgtct cagaattgga
     6541 catgatttcc ttaggaaccc aggataggtc aatttgatac tttccattta cagttagaac
     6601 agatactgga aaaattaatt catcatttgg tacaagctga aaatagattt tatctggaaa
     6661 ggtatcaatt ccatgttcag cttgttgttg ttttattgta tttttaaaaa attttacatg
     6721 atcttgcaat aaaataaaat gaatgattct accatcctca tcagaataat catcaatttg
     6781 tagattagat ttgaaaacat ctattccatt tatttgtgct ttataacttg gtgatatcat
     6841 ctcagtaaaa ttttttggaa aaaagatttc ctcgtgaaca acttgaagat gtgatatatt
     6901 ttttgtttcc cagttaaatg gcatttcaaa agttataatt ttttctaatg gttcatagac
     6961 aaaatttgaa atttcatcat agtaggattt gtgtctaaat tccacatcta tattttctga
     7021 gtttttgaat ttatgatatg ttgtatcaac tacactcaca tctgcaatat aaccggaaag
     7081 agaatctgta acattatcca cttcgtcaat ggaagttatg tctatttcat aatgatagag
     7141 accaccagat ttgaaatatg gctccaagta atcatattct ttgacatcaa tgatcagttc
     7201 accgtcagga gcaaaaaagg aatcatgtag gatcaactta ttatttttat aaatatcaat
     7261 aagaaatgtg acatttgaaa tattttcttc tgttttcttg tcaaaagcgt aaatacggat
     7321 ctttttattt tctgattcat caaaatagaa tggtaacatc tcaactgaaa caagaatttt
     7381 tctttcttca tagttcaaag atgttgtagt gtcgatacct aacccatgtc caaatgcttg
     7441 agagatagga atcagcatca aacctatcaa aattaaggct aattttcttg aattcatgat
     7501 gattatgcta aatgactcgt tagcttttta aaaacgatct gaattagaat ctgcttctca
     7561 ttcatagata tcaaaatttg ccttatgtta ataattttgc atctgtctaa aaaagttagc
     7621 catggctaaa ttttaaatat tgcaaattag tcgaacatat gtgttcacaa ttagccaaaa
     7681 aatggctatc gtcgtagcga tcgtttcttt tggatttgtg ggctatcttg tttttcctga
     7741 tgtgtatatt actgctcaag aacagccaga agtttttgta actcatactg gtgcagtggt
     7801 acaaaccact ggtgatgtat tagatcctgt ttatacaatt caagaagttg aatttgatcc
     7861 ggaaaaatac ttacgtgact ttgattatgg caaagtttcc caattagaag atggtacaat
     7921 acttcgtgag tataccattg tagctgagga tgataaaata atggaaattg caccaggcat
     7981 attttataat gtttggacat ttaatgggca ggttcctggt ccaacaattc gtgccaccga
     8041 gggtgaccta gtaaaaattt attttactaa taatgggagc aaggagcata caattcactt
     8101 tcacggaaac catccagctg agatggatgg tatttttgag atagttggtt ctggtggagg
     8161 tcagtttact tatgaattca tagcgggacc tgctggtgtt cacccatatc attgtcatgt
     8221 aatgccagtt gaggagcata ttggtcatgg actttacggt gtattcattg tagatccaaa
     8281 agagggaagg caacaagctg atgaaatggt tttgatgcta aacggatttg acactgactt
     8341 tgacggcgaa aataatctat atgctgcaaa cactattcca ttttattatc atcaccaccc
     8401 cattcaggtt aacacaaatg aactaatccg agtttatgtt gtaaacatgg tagggttaga
     8461 tcctatcaat aattttcatt tacacggaac cttgtacaag tactatccaa ctggtactga
     8521 tatgattcca tcggagttta ctgacatgac cacattttct caaagtgaaa ggggaattct
     8581 tgagtttgaa tatcaagata caggcagata catgttccat gcacatttga ctgaattttc
     8641 tgagaaggga tggtcaggtg ttttctatgc aaaagatagt tctcaactaa acatggaagg
     8701 atatgcttat ggaagttaag agatcgtcga atgtaaagac agcggtaagc gtcatcattc
     8761 cttttgtttt attagctata atgattggat atgtttttgg tccaggttct gaactgattg
     8821 gatatggtgt tgtattacca gaaatatcta ttgagaaagt tgaattcgtt gactcggaaa
     8881 taattgcaac tgtacgaaat actggacaga tttctgttaa tattgttatg gcagatatca
     8941 atgacagaat ataccccgct gctattgaac ctgataaaca tctggaacga ttcgagtcag
     9001 caattgttcg gataccattt gaatggaatg agggagaacc atatgctgta ggacttactg
     9061 tggatgatgg aaccagattt gaaaagcaag ttgatgtagc tgtacaatcg attaagccta
     9121 ccgttgaaat gatatcatat tttgctgtaa taggaacata tgttggaatt atccctgtgc
     9181 tgataggtct tctttggttt ccatttattt ctaaacttag ccgtagcaaa tacaaatttt
     9241 tcttggcact tactgttggt cttttacttt tcttaggaat tagtactgct gaggaagcca
     9301 ttgaaatatc tgccaataat ttgtctgatg ttttcaatgg agtactttta gttgctacgg
     9361 tagctgtagt gtcttttctt gctctgaatt atgtaggtga aaaactcaga aagcgtgcag
     9421 gtgcatcgaa acttgctggt cctgtagcaa ttgcattgat gattgcaatt ggaataggct
     9481 tgcataactt tggagaaggt cttgctattg gtgccgcaat tgtactaggt gaagctgcac
     9541 ttggagcttt tcttattgtt ggatttgcgc tacataatac tacagaagga tttgcaatag
     9601 ctgcacccat ggcacgtaca aagctgatga ttggaaagct tgctgcaatg ggaatgattg
     9661 ctggagttcc tgcaattttt ggtgcctggg ttggtggatt ttcttattca cctttagcag
     9721 cagttatctt tttagcaata ggtactggtg caatatttca ggtaattgtt tcaataatga
     9781 gatggataca aaatgaagaa ggtaaactct caaacagctc agtactagcg ggtattgctg
     9841 taggaatgat aattatgtat attacaagta ttctaatctg aaatctattc ttaattactt
     9901 ttcatcgaac agcaatttgc tttgttgata tttcgcattg cccaccagat tgaaaatgat
     9961 aatgcggcta tagatacaaa tttgagctct aagccttgaa gtgggaaaat tgctaatcca
    10021 ccaacaatac ctaaaaccgc tgttagtgat gttgtacatg ctggacatcc tggcgtaaat
    10081 gcagcgagtg caccaccaaa tattgctgat gatccaccct tttgcagatt cattctttgt
    10141 gttttgatat tgtatacaac taaggcaata ttgatcccac ccaaaattgc tacaattatt
    10201 gttaatccga tagaagctgc taggtattgg ggactaacaa ctagtgcaat taagttgtgg
    10261 tttaccgcgc ttgataaggt aagaaaatag tagattaaac caagtaaaaa gccagaaact
    10321 atagcaattc caccaaaact tttctgtttc agtatatctt tgattgctaa cttcaacata
    10381 atattctatt agtgattatg cttattaaat tcttgaatac taattccaaa ttttttcttt
    10441 aaatgtccac tttttgttta gtataaagtt actagatgca gcaatagcaa ctgcaaatac
    10501 aagtgccata ggataagtaa actgatagct atcaactaac acaaaaacca ttccaagttg
    10561 gactagtcca cctaacgaac taaatcctac aaattttcca tactgtatga cagtttttct
    10621 agcctcaaag ctcctatctc caaacgtcca aattttattc agaataaaat tcgaagacat
    10681 tgaaattatt attcctaccg tagtcgcatg gatataccaa aaattcatag ttgaatccag
    10741 aaacataagt gatgctaggt agttgatcaa aagtccagat gcacctacgg tgtaaaatct
    10801 agatgctttg gatataaagc gaacagagtt tcttgtctca ctattttctg ctttgcggcc
    10861 atacctgtac agtttccata cggatcttgc ataatctatc attgtagatg aatccaattt
    10921 actagatccg tgtactctat ttgtaaacgt gtaagggatt tcttttactt tcacaccctt
    10981 cgccttcact aaaatttcta agagaatttt atatccaatg gcatcaaatt ttaatccatc
    11041 aataattttt cgtctgaatg caaaaaatcc agacattgga tctgattggt ttactccaag
    11101 acctgttttc gcaatttttg ttgctgtctt acttaacaat tttcgtttag atgtccatcc
    11161 tttgatggaa ccaccagtca tatagcgtga cgcaattgcg atatcacatc tagatttttt
    11221 gatctcttcg attaatcttg gtattatttt tggtggatgt gaaaaatcac tatccattac
    11281 aataatggtc tctccacttg aatgttgaat accgttgagt atggcagagc ttaaaccatt
    11341 cttagcttta cggtgaatca catcaatagt atatccaacc ttttgtttca tttcatttag
    11401 ataagattct acaatttttc ctgttccatc aggtgaatta tcatcaacaa ctattgcttc
    11461 agcaagtaga ttttttggaa gatgttctcc aattgatttc aaaatttgta taatattctt
    11521 agattcgtta taggttggaa ttataattga aacctgtgca ttgttgctga gtcgtgaatt
    11581 ttgcatgttt cttcaactga aattttggtc attaatattt tttgtgtaat acatttcttt
    11641 accaagctta gctattttca cctgaaatta cgaagcacca gagagagatc agataggtta
    11701 tctgctgtct tacgaagaga atttttacca ttacccctta atcgttaaat tagtatgctt
    11761 agggctgaaa tagcgaaaag gaatattaac aaggatacag gatcctgatc aaatgaaagt
    11821 gataacatta agttctcttg tagtactttt tgcaatagca tcaatggtag caattgcacc
    11881 aaatgctttc gcagatcatc attctgcaac agtaaccaac gcaccaggat catcagtacc
    11941 aggttgtgag gaaacagctg acggttgttt cattccaaac actgtaacaa ttgatattgg
    12001 tggaatagtt acttgggaga acaatgatac agcagctcac acatcaacag gtggttcagc
    12061 aacagatggg ccaagtggtg tgtttgacag cagcttaatc atggcaggct catcattttc
    12121 tcatacattt gaagatgcag gaacattcga ttacttttgc atggttcatc catggatggt
    12181 aggaactgta attgttgaag acatggcagc cgcagcagcc gcagaagcag atgcagcaga
    12241 agcagcagcc gcagaagcag atgcagcaga agcagcagcc gcagaagcag cagccgcaga
    12301 agcagatgca gcagaagcag cagccgcagc aatgacagtt agagatccag cagtggactt
    12361 ggtagacact ctaagtcaca gtactagtaa tggttctgtt tcaagtataa tgactaacag
    12421 tgacgatgct actcttgtag ttgcaatgga tacatcagat gatggagaac tgtcaataaa
    12481 cctagacagt gattacatta ctgcatttga tgatggcagc tactttgttc tagtaaatgg
    12541 tgaagaggct gcatttagtc aagatggaaa tgacttaaca attccatttg aagctggaac
    12601 tgagaagatt gagatcgttg gaagcgttgt agttccagaa tttggaacaa tagccatgat
    12661 tgtactagct gtagcaatag taagtattat tgcaataact gcaaagacaa gaacaactct
    12721 aattcctaaa ctttaggaag ttctttttta tttttttatt ttctttttac cattgcgatg
    12781 atagctagta taactccagc agcaccaaga gatattccaa acacagcaaa gttgtatgct
    12841 gctccctcat cagaacctgt actagtactg ctttgtgctt ttattgacga aatatcttgt
    12901 tgaagagatg ttacagcatt tttaagagca gtaacttctt gtcctgatga tgaggtagac
    12961 gttggtggaa agtcaagaat agacgtactt tcaacatctt ctatgggaat tattgtgttt
    13021 atttgaacac cattaatctc tccggctagt ttcacttcaa gagaacctgt ctttgttgga
    13081 attatctttg cataataatg tccaggtctc ggatcagtgt ttatgtcaag taccttagaa
    13141 gctccacctg aaacaacaga tgcatccaat tttttaaatg cgttaattac accagtggat
    13201 acaccctcaa catctccagg ctctctaata tcgatagtaa tggcattcaa aatacccacg
    13261 actggaggtt catcttgcca tccgacttca atttcgtagg gatcgacttg aatggttgta
    13321 tgtgcatatg caaaagtaaa accaaatcct aaaacaacca agcccataat cgcaaaaaag
    13381 gcatatttca cgatttgcaa aacaaaccca tgtgttatta tctttactaa aatggtacaa
    13441 ctttcgaatt ggcaattctt taaattatga atatagtggt tttatgtaaa atgaacaaat
    13501 tactttttct accttttttg gtattgatta taggtatacc atctgcatat gcacatcctt
    13561 ttttacttga ttcagatcca ggacagggac aaaatgcagc cattggtaca acacaaatca
    13621 ttacttttta ttctgaagct atagaaattg acttttctga acttaaggtc tttgacagta
    13681 atggaaataa aattgataat atggatacgt catattatga tggagaaaat tctttagtaa
    13741 ttacaacgcc accacttgaa gacggcatct atactgtaac aagtaaagtc ctttcaaaga
    13801 tagatggtca tttggtgcaa gccgcaatca tctttggtgt tggtgaagta gaagttgacc
    13861 tatcacttct agaatcacaa gaagaatcag aaactacatt tctacctgaa gctgcagcaa
    13921 gatttccagg aatggttggt cagacaattg ttttaggatc tgcaatagca gctattgcaa
    13981 tctggggtac acaactgaag catttcggaa aagaaagtcg tgtcttattg aatcaagtat
    14041 atcgttccaa atttgctaag attactggaa tatctcttct tgcagtcttt acatctaatt
    14101 ttattatgtt aggaattcaa accatgcggc ttgaaacatc acctatgagt gttatagata
    14161 ctacatttgg caccacttgg ttaattcgga tgatcatcac ttcaattctt ttaggcatat
    14221 ggttttggat ggaaagaaaa caacatcttt caactaaaaa acacataccc atgcttatcg
    14281 catcgttgat gctaatattt acaacaacaa tgatggggca tggagcatca accgagcttt
    14341 ctgcaccaat catacttgat tatgtacata atttgttggc atcagtatgg ataggcggtg
    14401 taattttctt cggttttgtg ttattaccca cacttgcaag tattgatgga acacaaaaat
    14461 caaaggcaat tattgcaata ttacctagat attctggaat gatcataatt gcacttggta
    14521 tactaataat tacaggacca ttactgttat ggttcttaga gagtgacgta tcatctctga
    14581 caaattcaac ctatggttca ttgataattg caaagatcat gttagctata gtaatgattg
    14641 ggtttggagc attttaccaa tttaaaatcc aaaaaccagc tgaaagaaat ctaaagtcgg
    14701 gtaatatgtt cacatttaac aaactatcaa aactactcaa ggctgaagct atggttggta
    14761 ttgtgcttct tggagttgta gctctacttg ttaattcatc tcttcctgcc ggcgaaatcc
    14821 agacagtgga tgcacaagat ataacgtttg gattttcatc aaacttattt tcagaaaaag
    14881 ccgagtttga tgtcttactt atgcctgcaa gtgtaggtca gaacacaatc agtgttttag
    14941 tttcaaatgt ttctggggaa ccgttatcag acatttctaa tctgaagatc aaagtttcta
    15001 atccacaaag aaacattgca ccaattgaag ttcctatgat tgagcaaaaa ttagataatg
    15061 aaacacgctt tgaaggagat attacatttg gatttgctgg tatatggcag atagaaattg
    15121 aagcacaaag aattcaaaca gtaaacgagg gtatcatttt tgacattcta gtgaaaccga
    15181 cactttctga gattcgtact gaaattactg aatatgactt tccagatcct gactctgctc
    15241 cactttatcc cagatttgat ggtgataata caatatggat aagtgatgca gcaaagcctc
    15301 agctttggga atttacttta gatgatcaac agtttaaatc gcatgaattt aacggactta
    15361 ctaccatatt tattgacata gatcaaaacg gcaaggtttg gtttacggat acaccaaatt
    15421 ctatgattgg aagttttgat ccaaaaacag aaaaatttga aactattgaa atcccaatac
    15481 tagatattat ctctgaacaa tcaatcccga ttgcagtgaa agccgatttt gaagatagaa
    15541 tctgggttgc actagtagac aaaaataaac tattagcata caatcaaaat actaacaact
    15601 ttgataagtt ccttacaatt gaaacagaag aatctggccc atctgcactg ttagttgatg
    15661 atggtggtaa tgtatggttt gcagaagcat tagcaggtaa gattggtgtt gttgattcta
    15721 agacttttga aattactgaa tttgtaccag atgctccatt gaatgaacca tttgctttat
    15781 tatttgacaa atctggttcg ttatggatat ctgaacatac cggaccagga attacaaaat
    15841 ttgatcctat tctagaaaca tttgaacgtg taaaagcccc caatccagaa tctcttcctt
    15901 ttggattggc tattgataaa tatgataaca tttggtttgg tcaacatatt acagatgaac
    15961 tagctgtata tgacccctat aatgatcaat tgattgaagt ctccatccca acgccggaaa
    16021 gctttacaca atttacaaca tctgataatg atggcaatat ttggtttgtt gaacaaagag
    16081 gaaacaaatt aggcatggta tcgatttcta gcgttcctag tcaggcaaaa gctgtaattg
    16141 ttggtaacga tgaatttaat gtcaactata cagaaattgt atcaccatta attgcaggcg
    16201 gcatcataat ttcatcatta ttctttgtca aaagcgttca tgacaaaaga agaattgatg
    16261 ggttactaca aaatgatatt acgaaaccgt catgattccg tttgatatta accattgaag
    16321 tccagaaaca aatgcagaat caggaatatc tccactagcc caccatcctg cattattttt
    16381 gatccatcct ggaattacat cgtcagatc
//
LOCUS       DASCPJ010000014        14334 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000186444, whole genome shotgun sequence.
ACCESSION   DASCPJ010000014 DASCPJ010000000
VERSION     DASCPJ010000014.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 14334)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14334)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14334
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000186444"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(185..520)
                     /locus_tag="QGF01_00735"
     CDS             complement(185..520)
                     /locus_tag="QGF01_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19464.1"
                     /translation="MNNYTLLGIGVLAAFLISMNYAVPAFAQYGGQYGSEDTNTGAFG
                     SLEEQLELAKRKVEAAENNPATGSGTPYLDADGVFGASAIAGGIFGGIAAAFLIKGRQ
                     GRYAAYGRG"
     gene            640..1089
                     /locus_tag="QGF01_00740"
     CDS             640..1089
                     /locus_tag="QGF01_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HJN19465.1"
                     /translation="MGRLHTHNHGKAHSIRPIESKFSSWVKQDTKEIEDLVIKYTKDG
                     LTPSQIGIKLRDQHAIPLVKPIINKTISDILEQNDLKSEIPEDLNNIVTKAVGLQKHL
                     KSNKNDRRNIRSLELIEAKVHRLSIYYKKIGRISKNWKYKSVIAQLE"
     gene            1089..2510
                     /locus_tag="QGF01_00745"
     CDS             1089..2510
                     /locus_tag="QGF01_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HJN19466.1"
                     /translation="MVKSLNESLSYFKHTISDLIKSGKNISIISHLDCDGLTSASIIG
                     KALFREGIRCTISTTNEFNNNLVDRLSNDSRDFHIITDLAGGFANTLDEKLGEDWLVL
                     DHHEIPEEEYDNERVINSWKFGIDGGLEICAGGMTYLASKSLNKKNEDLAAIAIISAI
                     GDRQDKGERKSFTGKNLDILNTAKSNGQIDVDLDLLLVGRETRPLSDALAFTSQPFIE
                     GLTWNRSSCLALLNSSGIPLKEKGRWRVPAELSDDEKRHLIETISKFATGKNMTEILE
                     ELIGYTFTFPKEDQRSFLRDVREFSTVLNSCGRISKSGVGISICMGDRHRMLQEGEKI
                     LSEYRKKIRDYMNVLSNERWRTAENMNCVMVNGEGVVPEMMTGTITSIIAGSPKNTGK
                     IVILRTNGEEGMIKFSSRKSNTCNLNINLSSLMRTGAAKFDGVGGGHDAAAGAKITKD
                     KLDDFLDYLEGNVTNLQDPNNTQ"
     gene            2473..2715
                     /locus_tag="QGF01_00750"
     CDS             2473..2715
                     /locus_tag="QGF01_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HJN19467.1"
                     /translation="MSLTCKIQITLNRLSDEKAVAIKKALEPDNIDFPKDLSLEIENL
                     GDQLVVSFKNRGDTKKLISTVDEVLEHIQLALKVIE"
     gene            2715..3977
                     /gene="serS"
                     /locus_tag="QGF01_00755"
     CDS             2715..3977
                     /gene="serS"
                     /locus_tag="QGF01_00755"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HJN19468.1"
                     /translation="MLDMKIVRQDPEKIQKMLRDRYVEFDLDSLLDLDKKRREYILST
                     DDLRKKKNEMSLKIAETKKAGQDADKIIQEMQLVSRQLTELEETQNKTELEYSKLILT
                     LPNIFDQTVPIGEDSSANKEVKKWGDIPKFDFQVSDHIDITQKLNLVDLQRAAKTAGA
                     RFYYLRGDLVKLNQSLIQFALDFLDDKGFTLFQPPYMINKKSMEGAIIAEDFKDVIYK
                     IDDEDLYLIGTAEHAMASMYADEILDGASFPMRCAAISPCFRKEAGAHGKDQKGIFRV
                     HQFEKVEQFVFSRPEDSWKEHEKMIAITEEFFQKLEIPYRVMLLSSGDMGKISAKTYD
                     LETWMPGQNAYRETASCSNCLEYQARRLKIRFRDRTNEDTKYVNTLNSTLVATERTMV
                     AIIENFQTKDGHIEIPKQLQKYVGKKMI"
     gene            4019..4633
                     /locus_tag="QGF01_00760"
     CDS             4019..4633
                     /locus_tag="QGF01_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HJN19469.1"
                     /translation="MVRRKTRKIKDKWKEKKWVTVLAPDAFNNIPIAYIPVTSDETAK
                     GRVLEVTLFEILKGDPSQHQYKLFFQVDKVNQEKATTIFKRYEYAKEFLRSLIRRGSS
                     RINFILDAKTKDGYIFRIKMLALSHRELNTSRKRALRLIAKDVLEKTVRNMTLDQFVQ
                     ASCYGKINSDIMAAAKKVIHLRHVGLEKVKLVRTAEAEITLLQA"
     gene            4630..5076
                     /locus_tag="QGF01_00765"
     CDS             4630..5076
                     /locus_tag="QGF01_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013994.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HJN19470.1"
                     /translation="MIIQKNLKNQKKLDVTIELILHATENFQKVYDAFFDLFVIEKDD
                     ISKQELVGHFGNPIFMLHIQIKKIKAYEVVKKLISIMPKSELDLILKDLENRIDNSSL
                     YLRFSKQNFLENIIKLEEKDPIRMKIYTPVYVKKEIVDVYRKLLET"
     gene            5137..6600
                     /locus_tag="QGF01_00770"
     CDS             5137..6600
                     /locus_tag="QGF01_00770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJN19471.1"
                     /translation="MDTDYDVIVAGGGLTGTIVAQAVSHYSNRNLSILSIDRNPENLP
                     GRKSNPGWTCGDACSKEAVDFMTERIKVPWTSPEIEHNVKGVMAFSPDKETAIPFDGD
                     GYMLNRQKLPEIQNARAKKMGVNFDFEINLTGLIYDELQVIGVQGINNKTKQPYKKTA
                     KVVVDATGVTSMLRNQLKNSTKVEKKIDRRDLESTGRHIMYFEKGEEDLTEFDPNYCI
                     IHLDQDIAPGGYGWVFPKADNKVNIGLGVEKSILDQRNQRLGKKDNVASLMEEYLQRN
                     RAIKNAKLSQDPEDIHNNTGVFQVSVRRQNDCMVSGGYMLVGDSAWMPKPIDAGGIGP
                     ALIAGTILGKNVVDAIQANDVTEKGLWQYNLDYIEEYGYKTAGLELFRRLVQTLTNEQ
                     ISYGMKHFLGNLDVESISKGEHPDFSGLTKLGMIIRGAMNKTVASGLKYTSEQNQWLV
                     EHYRNYPKEPEGFNEWNKSLLKTLDESFVKIATYSKN"
     gene            7101..7457
                     /locus_tag="QGF01_00775"
     CDS             7101..7457
                     /locus_tag="QGF01_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJN19472.1"
                     /translation="MQTVLNGVPIEQKEKIEVILQIMADKYSRDLLRITQDFPKSAFK
                     IADETGIPISTVYRRLQKLQNNDVIRVSGEITMEGKKHFLYQSKVKSISSKVTGDFIH
                     VEVIPNPPSAPHKWGV"
     gene            7563..8090
                     /locus_tag="QGF01_00780"
     CDS             7563..8090
                     /locus_tag="QGF01_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19473.1"
                     /translation="MSYRLPSKLLDDLETEAREKNMSQNMLVKQILMKYIGWDRFAQK
                     MGMIPVPQKILESLGGDISNQTILKIIDTLFTIIKDSVMLMKGGYDLRRTIETLEDYM
                     RGSGMESDHRIDGDTHHFIIQHKMGMQWSIFTELLLRRVFTEFEKGDDITFQTTESTV
                     IATIPLGSDFDEHNY"
     gene            complement(8101..8466)
                     /locus_tag="QGF01_00785"
     CDS             complement(8101..8466)
                     /locus_tag="QGF01_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19474.1"
                     /translation="MSQAESIYDICLAKLRMNHNNIDPYLLMKIMYLERTVSTNIALG
                     QKGELPNVPPMVEIEIKFKEGTDTEKVIYEMQSRGIPAMHHGKEIFMKSTMSASDLCD
                     LASNPDVEMIHGNATPASW"
     gene            8620..9447
                     /locus_tag="QGF01_00790"
     CDS             8620..9447
                     /locus_tag="QGF01_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN19475.1"
                     /translation="MSDQNEQSVDESEQSTYLDWNNSINILDSAFEKGLFVLVIGPKG
                     TGKTSLVREYAAQKSKKLESINFSLRTRESHLIGTKSLSEGSIGFDEGVLVKSMKEGS
                     MLYLDELNAAEADVLLRLDEALDDRRQIVLKESDGQVIKADDSWFAVATINPLTHVGT
                     KELPPQLLSRFPVRIRLDYPPEDVEYKIIKKHVKNLHETEILQGIKLANTLRQASAVE
                     ELYYSPSMRETIAYAKLLEGGSSAKEAAKIVFGNVYSQWGNVEFQKVNDIITSMFGS"
     gene            9447..11015
                     /locus_tag="QGF01_00795"
     CDS             9447..11015
                     /locus_tag="QGF01_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HJN19476.1"
                     /translation="MQSIQLRNDTLIDIATFLVRRWSEKENVTVEFSKIKQSETRLKE
                     KRVLLIPNDRYYGDSFQKYRQFRTSIWYEGMRIRHCKKILSNDHAYGFILNTIETRRI
                     ELLGIKIWKGMTEELIFNYTNMWLSRNNLSSVFGKSRMIEAFYQYFLFGDVKGEVQPS
                     NFDKVVKAVKFAKNILNDSIKNDYDTEWIEKKIPEILKILDVDALISIPISVPLRGPG
                     IAITPNDFIKAMKQVGKSREGDFGKIDPENILQGKAVSEEFKVIEEENKKNEKKGLSS
                     GTIGIQIPEMSNADETKIFDQDLINNLKTRFKEWKTGWKEYHSFHGDEFDSDVYLEGF
                     DQPFITDLKKSIKTRIVILLDHSSSITDQQIDYKKATLALCEVLAFLKIKFSVYAFNT
                     TGRQVVCWLVKPEDLKWNNSCAKRLAKIPANGGTPLAEVYDKMYPILRSKKPDIFLTL
                     SDGEPSDPYAVRSMVKSLKSLGIKMVAIGVGRDTSNATIIATNLKYLGFERTLRVSRL
                     NDIPKKVLTVLGNN"
     gene            11029..11259
                     /locus_tag="QGF01_00800"
     CDS             11029..11259
                     /locus_tag="QGF01_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19477.1"
                     /translation="MEKECSECGSLFICEEDSGTCWCASLPKLSKEQITDNDCMCKKC
                     LLQKYKKKILKTDEITKDLFEEEFRYQWKNKH"
     gene            complement(11263..11634)
                     /locus_tag="QGF01_00805"
     CDS             complement(11263..11634)
                     /locus_tag="QGF01_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19478.1"
                     /translation="MVGLFNKNKPQQKLRKFVKEKKYAEALKYSVNLEKDEKYRNDTD
                     IAFIIGTIHFLKGDNEKTLSYMNKVLEIAEYDIDALVLKASILADMKNKKGVMECCDK
                     IKEIDPKNTTMQKILYQFNEL"
     gene            complement(11655..12320)
                     /gene="fsa"
                     /locus_tag="QGF01_00810"
     CDS             complement(11655..12320)
                     /gene="fsa"
                     /locus_tag="QGF01_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HJN19479.1"
                     /translation="MKIFLDTANLEIIRMYNDMGLVDGITTNPSLLAKEGGDPQKIME
                     EISEIIKGDVSLEVISTDYDGMMEEGRRLRKYGDNVVVKCPMTGEGLKACKSLTAEGI
                     PVNVTLIFSPNQAVLAAKAGAKYVSPFIGRLDDIGHTGMDLIKEIKQIFQNYDFNTEI
                     LVASIRHPQHVVDAAKIGADIVTVPGAVLGKMLTHTLTDKGLKAFLDDWEKLKSENLN
                     ADN"
     gene            complement(12317..13291)
                     /locus_tag="QGF01_00815"
     CDS             complement(12317..13291)
                     /locus_tag="QGF01_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HJN19480.1"
                     /translation="MSTEHLGDMRTEYSKALVAVGEQDPNVVVLGADTTDSLKTSNFG
                     KKFPERFFNVGIAEANLVSVAAGLAYSGKTAFASTYAIFLPGRCVDQIRNAIAYPSPG
                     DKKGLNVKLVVSHGGLSVGADGGSHQQIEDIAIMRVIPNMRVLVPADSTTVSKLTWII
                     SQQYGPFYMRMARSKTPIIHNDSQEFQIGKGIVLRDGSDCTIAACGITVKMALDAANM
                     LQQDGISCRVIDCFSIKPIDKDLLEKSARETGSIVTCEEHNIIGGFGSAVSEVVSESY
                     PVPIKRIGAQDKFGESARDNEIPQLLEKHGLTSINIAKAVKEVRSKKT"
     gene            complement(13288..>14334)
                     /locus_tag="QGF01_00820"
     CDS             complement(13288..>14334)
                     /locus_tag="QGF01_00820"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HJN19481.1"
                     /translation="SRWKTGDKWRSFGWNVIEVDGHRTEQISDAIRRANETKGLPSII
                     IARTVKGKGVEHMEDNPQWHGKAPQQDIVPIIEDELESQFMIAPSIIAGDMSNLGKEV
                     ERCNEGRADYIHLDVMDGQFVPNKTFDHTEIQKLRPLSLLPFDTHLMINDPVKHVRNY
                     IDAGSDIITVHAEVCDESSFGEICDQLHSNQVGIGLAINPDTDLPQWSYKFAEMLDQI
                     IVMSVVPGKSGQKFIESTHEKIQRITKDLHDHKFDGYIEVDGGVNLENIGACFADGGR
                     AFVGGSAIIGQSDVRMIIKEFRNQVLDSRRRLLIKKAHDLGGTDLVNKWIDLHVVGKK
                     KDGLVQIAKELGFQ"
BASE COUNT         5060 a         2316 c         2548 g         4410 t
ORIGIN      
        1 tcgtagcttg gtcctatttg tccagagaga ctagatagaa tacttagtac tgtcccaaat
       61 ataggaatca tcaataaaat gactggttgg aatgcatata tacatttctg ttattttcag
      121 atttaacata ttctgcgcac tataatctaa ctatatgaaa taaaataagg aaagaggaaa
      181 aattttaacc gcgtccgtat gcagcgtatc tgccttgtct tcccttgata aggaaggctg
      241 cggcaattcc gccaaatata ccaccagcaa tagctgatgc accaaataca ccatctgcgt
      301 cgagatatgg tgttcctgat ccagtggcag gattattctc tgcagcttcg acttttcgtt
      361 ttgcgagttc caattgttct tcaagtgatc caaaggcacc agtgttagta tcttctgagc
      421 catactgacc accatattga gcaaatgctg gaacagcata attcatactg atcaagaaag
      481 cagcgagaac gcctattccc aataatgtat aattgttcat tggtatctat caaaatttga
      541 tatatgttat tatttaactt tttctcactt tcagcgttga aagacactaa gatctagtat
      601 taagtaacat ttatgttagt tttttagagg tttaacctaa tgggaagact tcatactcat
      661 aatcatggaa aggcgcattc aataagacct atagaatcaa aattttcatc atgggtcaaa
      721 caggatacaa aagagattga agatttagtt atcaagtata cgaaagatgg tcttacacca
      781 agtcaaattg gtataaaact cagagatcag catgccatac ccttggttaa accaattatc
      841 aataaaacaa taagtgatat tttggaacaa aatgatctga aatctgaaat accagaagat
      901 ttgaacaata ttgttaccaa agctgttggt ctacaaaaac acctaaaatc taacaaaaat
      961 gatagaagga atatacgttc acttgaactg attgaagcca aggttcatcg actgtcaata
     1021 tattataaaa aaatcggtcg tatatctaaa aattggaaat ataagtctgt aatcgctcaa
     1081 ttagagtgat ggtaaagagc ttaaacgaat cactatcata ttttaagcat acaatttctg
     1141 acttgattaa atcgggaaaa aacatctcaa taatttcaca tcttgattgt gatggactaa
     1201 cttcagcaag cataattggt aaggcgcttt ttagagaggg gataagatgt actattagta
     1261 ctaccaatga atttaacaat aatttagttg atagactatc taacgattcg agggattttc
     1321 atataatcac tgatctggct ggtggttttg ctaatacatt agatgaaaaa ttgggtgaag
     1381 attggcttgt acttgatcac catgaaattc cagaagagga atatgataat gaacgtgtaa
     1441 ttaactcgtg gaaatttggt attgatggag gcttggaaat ttgtgctgga ggaatgacat
     1501 atttagcatc aaaatcactc aacaaaaaaa atgaagatct tgccgccatt gctataattt
     1561 ctgctatagg tgatagacag gacaaaggag agagaaaatc atttactggc aagaacttag
     1621 atattctcaa tacagcaaaa tctaacggcc agattgatgt ggatttagat ctacttcttg
     1681 ttggcagaga aactcgacca ctatctgatg cacttgcatt tacttcacag ccattcatcg
     1741 aaggactaac ttggaataga agttcttgtt tggcattgct taattcttca ggtattccac
     1801 taaaggaaaa aggaagatgg cgtgttccag ctgaattatc tgatgatgaa aaacgtcatc
     1861 ttattgaaac tatttccaaa tttgctacag gcaaaaatat gacggaaata ttggaagagt
     1921 taattggcta cacttttaca tttccgaaag aggatcaaag aagtttctta cgagatgtta
     1981 gagagttttc aactgttttg aattcatgtg ggcgtataag caaatctggt gtgggtatat
     2041 cgatctgtat gggtgataga catagaatgt tacaagaggg tgaaaagatc ttatctgaat
     2101 ataggaaaaa aatccgcgat tacatgaatg tattatcaaa cgagcgatgg aggactgctg
     2161 aaaatatgaa ctgcgttatg gtcaatggtg aaggagtagt acctgaaatg atgacaggca
     2221 caataacatc aattattgcc ggttcaccca aaaatactgg taaaattgta atattacgca
     2281 caaatgggga agagggaatg atcaagtttt cctcacgaaa atcaaatacc tgtaatctga
     2341 acataaatct gagctcatta atgagaacag gggccgcaaa atttgatggt gttggaggag
     2401 gccatgatgc tgccgcagga gccaaaataa ctaaagacaa attggatgac tttttagatt
     2461 atttagaagg aaatgtcact aacttgcaag atccaaataa cactcaatag actttcagat
     2521 gaaaaggctg ttgcgataaa aaaggcgtta gagccagata atatcgattt tccaaaagat
     2581 ttatcacttg agattgaaaa tcttggtgat caacttgttg ttagttttaa gaatcgggga
     2641 gatacaaaaa aattgatctc aactgttgat gaagttttag aacatattca acttgcgtta
     2701 aaggtgattg aataatgtta gatatgaaaa tagtcagaca ggatcctgag aaaatccaaa
     2761 aaatgctcag ggatagatat gtagaatttg accttgattc attactagat cttgataaaa
     2821 aacgaaggga gtatattctt agtactgacg atcttagaaa gaagaaaaat gagatgtcgc
     2881 taaaaattgc tgaaacaaaa aaagccggtc aagatgcaga taaaataatt caagaaatgc
     2941 agttagtttc tcgtcaactc actgaattag aagaaactca aaataaaaca gagttagaat
     3001 attcaaaatt aatattgaca cttcctaata tttttgatca aacagttccc ataggtgaag
     3061 atagttcagc aaataaggaa gttaaaaaat ggggtgatat tccgaagttt gattttcagg
     3121 tgagtgatca tattgatatc acccaaaaac tgaatcttgt ggatctccaa agggcagcaa
     3181 aaacagctgg agcaagattc tattatctga gaggtgattt agtaaaatta aaccaatcat
     3241 taattcaatt tgcgttagat tttttagatg ataaaggatt cacattattt caaccaccat
     3301 atatgattaa taaaaaatct atggagggag ctatcatagc tgaagatttc aaagacgtta
     3361 tctacaaaat tgatgatgaa gatctatatc taattggaac tgcggaacat gctatggcat
     3421 caatgtatgc agatgagata ttagatggag cctcatttcc gatgagatgt gctgcaataa
     3481 gcccttgttt tagaaaagaa gctggagctc atggaaaaga tcaaaaaggg atttttcgtg
     3541 tgcatcaatt tgaaaaagtt gaacaatttg ttttttccag accagaagat tcatggaaag
     3601 aacatgaaaa gatgattgct ataacagaag aattttttca aaaacttgaa attccatacc
     3661 gtgtgatgct tttatcaagt ggtgatatgg gaaaaatctc cgccaagaca tacgatctag
     3721 aaacctggat gcctggtcaa aatgcctaca gagaaacagc atcatgttca aattgtcttg
     3781 agtatcaagc tagaaggcta aagattagat tcagagatcg gacaaatgag gatactaaat
     3841 atgtcaatac actcaacagc acacttgttg caacagaacg aactatggtt gcaataattg
     3901 aaaactttca aacgaaagat gggcatattg aaattcctaa gcaattacaa aagtatgttg
     3961 gaaagaaaat gatctaaagg tggcacactt atattttgga atttgaggtc gatagtaatt
     4021 ggttagaaga aaaacacgaa aaataaaaga taaatggaaa gagaaaaaat gggtgacagt
     4081 tttagctcca gatgcattta acaatattcc aattgcatac ataccagtaa caagtgatga
     4141 aacagccaaa ggaagagttt tagaggttac attatttgag attttaaagg gtgatccttc
     4201 acaacatcaa tacaaattat ttttccaagt ggataaagtt aatcaggaaa aagctactac
     4261 aatttttaag agatatgaat atgccaaaga gtttcttaga agtttaataa gacgtggttc
     4321 gtcaagaatt aattttattt tagatgctaa aactaaagac ggatatattt ttagaatcaa
     4381 aatgctagca ttgagccata gagaactcaa tacgtctaga aaacgtgcat taagactaat
     4441 tgcaaaagat gtcctggaga aaaccgttag gaatatgaca ctggatcagt ttgtgcaggc
     4501 ttcatgttat ggaaaaatta attcagacat aatggcggct gcaaaaaaag ttatacatct
     4561 cagacatgtt ggtttagaaa aagttaaact tgttagaact gcagaagcgg aaatcactct
     4621 actccaggct tgataattca aaaaaattta aaaaatcaga aaaaacttga tgttacaatt
     4681 gagttaatat tacatgcaac tgaaaatttt cagaaagtgt atgatgcgtt ttttgatctt
     4741 tttgtaatag aaaaagatga tatttcgaag caagaattag ttggtcattt tggaaatcca
     4801 atatttatgc ttcatataca aataaaaaaa ataaaggcct acgaagttgt aaaaaaactc
     4861 atttcaataa tgccaaaatc tgaactagat ctaatattga aagatctaga aaatcgaatt
     4921 gataattcat ctctatatct acgtttttca aagcaaaact tccttgaaaa tatcataaaa
     4981 cttgaagaaa aggatccaat ccgaatgaaa atctacacac ctgtatacgt aaaaaaggaa
     5041 atagtagatg tatatcgaaa actgcttgaa acataaccca ataaattttg gtttttatga
     5101 gtattgtttt aaataaagtt gtaagattga agaattatgg atactgatta tgatgttata
     5161 gtagcaggtg gaggtctgac tggaacaatt gtagctcaag ctgtatcaca ttactctaat
     5221 aggaatttat ccattctttc aattgacaga aatccggaaa acctccctgg acgaaaatca
     5281 aaccctggtt ggacatgtgg tgatgcttgt tcaaaggaag cagtagattt catgacagaa
     5341 aggataaaag ttccttggac atctccagaa attgagcata atgtaaaagg agtgatggca
     5401 ttctcacctg ataaagaaac tgcaattccg tttgatggtg atggctatat gcttaatcgt
     5461 caaaaactac ccgaaatcca aaatgcgaga gcgaaaaaaa tgggagttaa ctttgatttt
     5521 gaaatcaatc ttacaggtct aatctatgat gaactacaag tgataggagt tcaaggtatt
     5581 aataacaaaa caaagcaacc atacaaaaaa actgcaaagg ttgtagtgga cgcaacagga
     5641 gttacttcaa tgcttagaaa ccagcttaag aactctacca aagttgaaaa aaaaatagac
     5701 agacgtgatc tagaatcaac aggaagacat attatgtatt ttgaaaaggg tgaagaagat
     5761 ctcacagagt ttgacccaaa ttattgtatt attcacctag accaagacat tgcacctgga
     5821 gggtatggct gggtttttcc aaaggctgac aataaggtga atataggatt aggtgttgaa
     5881 aaatcaattt tagaccaaag aaatcaaagg cttggaaaaa aagataatgt tgcatcgctg
     5941 atggaagaat atcttcaaag aaacagggcg attaagaatg caaaactatc acaagatcca
     6001 gaagatattc acaataacac gggagtattc caagtttctg taagaagaca aaatgactgt
     6061 atggtttctg gaggctacat gttagttgga gattctgcat ggatgccaaa accaattgat
     6121 gctggaggaa taggtccagc cttgattgct ggtacaattc ttggtaaaaa tgttgtagat
     6181 gcgattcaag ctaatgatgt tacagaaaaa ggattatggc agtacaatct tgactatatt
     6241 gaagaatacg gttacaaaac tgctggactt gaacttttta gaagactagt acaaacgctg
     6301 actaacgaac agattagtta tggtatgaaa cattttttgg gaaaccttga cgttgaatct
     6361 atcagcaaag gtgaacatcc agatttttca ggattaacaa aactaggtat gattattcgt
     6421 ggtgctatga ataagacagt tgctagtgga ctaaaatata catcagaaca aaatcaatgg
     6481 ttggttgaac attatagaaa ttatcctaaa gaacctgaag gatttaatga gtggaacaaa
     6541 tctcttctca aaacacttga tgaatcattt gtaaaaatag ccacatactc aaagaattag
     6601 tagagctgga tcagatttat tttgatctaa tggacttgat gatatatcat aatttccttg
     6661 tttttgaggc caatacatat tttcaaatat gaaaatctac gatttcgctt tattccttgc
     6721 tgagtgaatt atattatgaa aaaaagatgc cttgtttgta ataatgaact aaaaaacgaa
     6781 aatgctacac attgttcgga aaagtgttta ttcaagagtg ttgaaaaaag caagtctatt
     6841 ttagaaaata atcacaaata atatactatt catcatttaa tttgtatgct acaattgttt
     6901 gagtgtctga atttttcttt cttttttttt actaattccc cattcatcct ttgtatgacc
     6961 gaaatttgat tcagataggt tttcttcttt cttagcatca accataatca aacaagttta
     7021 tcataagata taatcgacaa gtatacaatg tgttgcaccg taaacaactg tgttatattt
     7081 aaactacaag tatactacac atgcagactg ttcttaatgg tgttccaata gagcaaaaag
     7141 aaaagattga agtaattctg cagattatgg ctgacaaata ctctagagat ctgcttagaa
     7201 tcacacaaga tttcccaaaa tctgctttca aaatcgcaga cgaaactgga attccaatta
     7261 gtactgtgta tagaagatta cagaagctcc aaaataatga tgtgattaga gtttctggtg
     7321 aaatcactat ggaaggaaaa aaacactttc tttaccaaag taaagtgaaa tcaatttctt
     7381 ccaaagttac gggtgacttc atccatgtag aagtaatccc aaaccctcca tctgctcccc
     7441 ataaatgggg ggtttagttc ttctatacac tataattaat aaccccacaa cgcattgtta
     7501 cattattgac gtcaacgact agacgaaaac cacatacaaa aataaaggaa gaaactgata
     7561 ctatgagcta tcgcctcccg tcaaaactat tagatgacct ggaaactgaa gcaagagaga
     7621 aaaacatgtc tcaaaacatg ctcgtaaaac aaattctcat gaaatatata ggatgggata
     7681 gatttgcaca aaaaatggga atgattccag ttccacagaa aattctcgaa agtttaggcg
     7741 gagatatatc taatcagact attcttaaaa tcattgatac tctgtttaca attataaaag
     7801 attcagttat gctcatgaaa ggtggttatg atctaagacg cactatagaa acacttgagg
     7861 attatatgcg aggctctggt atggagtcag accatcgcat tgatggtgat acacatcatt
     7921 ttattattca gcataagatg gggatgcaat ggtcaatttt taccgaacta ctgcttagga
     7981 gggttttcac tgaatttgaa aaaggtgatg acataacatt ccaaaccaca gaatccactg
     8041 tgattgctac tataccattg ggttctgact ttgacgaaca taattactaa gttctaaaca
     8101 ttaccatgac gctggagttg cattaccatg aatcatttca acatctggat ttgaagcaag
     8161 atcacacaaa tcactagctg acattgtaga tttcatgaat atttcttttc catgatgcat
     8221 agctggaatt ccacgagatt gcatttcata aattactttc tctgtatctg ttccttcctt
     8281 gaatttaatc tcaatctcta ccataggtgg tacattagga agttctccct tttgtcccaa
     8341 tgcaatattt gtagaaacag ttctttcaag atacataatt ttcattaaca aataaggatc
     8401 aatgttatta tgattcattc ttaattttgc aagacaaata tcataaatag attctgcttg
     8461 tgacactaaa tttatgataa aaatatttga attaaataga acagtagatt tccaaaaatg
     8521 aaaattacag ttagatcaga tttagcctca tatcttgtat ataaaaaaat actgggctga
     8581 attagtctta aataaagtat ccatctcaag gatattactt tgtcagatca aaacgaacaa
     8641 tcagtagatg aaagtgagca atcaacttac ctagattgga ataactcaat caatatttta
     8701 gacagtgcgt ttgaaaaagg actatttgtt ttggttatag ggccaaaagg aaccggtaaa
     8761 acttcgttgg taagagaata tgcagcacaa aaatcaaaaa aattagaatc aatcaatttt
     8821 agtcttagaa caagagaaag ccatctgata ggaaccaagt cattatccga aggaagtatt
     8881 ggttttgatg aaggtgtttt ggtaaaatca atgaaagaag gtagtatgtt atatcttgat
     8941 gagctcaacg cggcagaagc tgatgtttta ttgcgattag atgaggcatt agatgatcgt
     9001 aggcagattg ttctcaagga atctgatggt caagtgatta aagcggatga ctcgtggttt
     9061 gcagttgcaa ctattaaccc tctaacacat gttggaacaa aggaactacc gcctcaactt
     9121 ctaagtagat ttccagttag aataagattg gattatccac ctgaagatgt ggaatataaa
     9181 ataataaaaa aacatgtaaa aaatctacat gagactgaga ttttacaggg aatcaaactt
     9241 gcaaatacat tacgtcaagc atctgctgtt gaagaattgt attattcccc atctatgaga
     9301 gaaaccattg catatgcaaa attattagaa ggtggatcct cagcaaagga agcagcaaag
     9361 attgtttttg gaaatgtata ttctcaatgg ggaaatgtag aatttcaaaa ggtaaatgat
     9421 atcatcactt caatgtttgg tagctaatgc aatctatcca actacgaaac gatactttaa
     9481 ttgatattgc cacatttctt gtaaggcgtt ggtcggaaaa agaaaatgta acggtagaat
     9541 tctcaaaaat aaaacagagt gaaacaagat taaaagaaaa aagagtgtta cttattccaa
     9601 atgatcggta ttacggagat agtttccaaa aatatagaca gtttagaact tcaatttggt
     9661 atgaaggtat gcgaataaga cattgtaaaa aaattcttag taatgatcat gcatatggat
     9721 ttattttgaa tactattgag acccgtagaa ttgaacttct aggaataaaa atttggaaag
     9781 gaatgactga agaattgata tttaattata caaatatgtg gctatctagg aacaacctaa
     9841 gttcggtttt tggaaaatct aggatgatcg aagcattcta tcaatatttt ttatttgggg
     9901 atgtgaaagg tgaagtgcaa ccaagtaatt ttgacaaagt agttaaagca gtaaaattcg
     9961 ccaaaaatat tcttaatgat tctattaaaa atgattatga tacagaatgg atagaaaaaa
    10021 agatccccga aattttaaag attttagatg ttgatgctct tatctcaatt cctatatctg
    10081 ttccattaag aggacctgga attgccataa cacctaacga ttttatcaaa gcaatgaaac
    10141 aagttggtaa atcaagggaa ggagattttg gaaagattga cccagaaaat attttacaag
    10201 gaaaagcagt ttctgaagaa tttaaagtaa ttgaagaaga aaataagaaa aatgagaaga
    10261 agggtttgag tagtggaaca ataggaatac aaattcctga aatgtctaat gctgatgaaa
    10321 ctaaaatctt cgatcaagat ttgataaata atttaaagac cagatttaaa gaatggaaaa
    10381 ctggatggaa agaataccat tcttttcatg gtgatgaatt tgatagtgat gtgtatctcg
    10441 aaggttttga tcaaccgttt ataacagatc ttaagaaatc gattaaaaca cgaatcgtaa
    10501 ttttacttga tcattcatcc agtattactg atcaacagat agattacaaa aaagccacat
    10561 tagctctatg cgaagtccta gcttttctaa agatcaaatt ctctgtatat gcatttaata
    10621 caactgggag gcaagttgtc tgctggcttg ttaagccaga agatcttaaa tggaataatt
    10681 catgtgcaaa aagactagct aagattccag caaatggtgg aactccacta gctgaagttt
    10741 atgataaaat gtatcctatt ttgcgttcca aaaaaccaga tatttttttg acactctcag
    10801 atggagagcc ttcagatcca tatgcagttc gttcaatggt taaatcactc aaatctcttg
    10861 gaatcaagat ggttgcaatc ggagtgggta gagatacctc taatgcaaca ataattgcaa
    10921 ctaatttgaa atatctagga ttcgaaagga cgttgagagt ctccagactt aatgatatcc
    10981 ctaaaaaggt gttaactgta ctaggtaata attgaggttt tagataaatt ggaaaaagaa
    11041 tgttctgaat gtggaagttt gtttatttgt gaagaagata gcggtacttg ttggtgtgcc
    11101 agtttgccaa aattatcaaa ggaacagatt actgataatg attgtatgtg caaaaaatgt
    11161 cttttacaga aatataagaa aaaaatccta aaaacagatg aaattacaaa agaccttttc
    11221 gaagaagaat ttcgctatca gtggaaaaat aaacattaat ttttaaagtt cgttgaattg
    11281 gtagagtatt ttttgcattg tagtattttt aggatctatc tcttttattt tatcacaaca
    11341 ttccattacc ccttttttat ttttcatatc ggcaagaata ctagccttaa gcacaagggc
    11401 atcaatatca tattcggcaa tttctagaac tttattcata tatgataagg ttttttcatt
    11461 atcacctttc agaaaatgga ttgtgcctat aatgaatgca atatcagtgt catttctata
    11521 tttttcatct ttttcgagat ttacactata ctttaatgcc tcagcatatt ttttttcctt
    11581 gacaaattta cgaagtttct gttgaggttt atttttattg aaaagtccta ccaattttga
    11641 tacaaattaa aattttagtt atcagcattt agattttctg atttgagttt ttcccagtca
    11701 tccagaaaag ccttcaagcc tttatcagtt aatgtatgag taagcatttt tccgagaact
    11761 gcgcctggaa ctgttacaat atctgcgccg atttttgcag catcaactac atgttgtgga
    11821 tgcctaatac ttgcgactaa aatctctgta ttaaaatcat aattttgaaa aatttgtttg
    11881 atttccttta tgagatccat gcccgtatga ccaatatcgt ctaatcgtcc aatgaaagga
    11941 ctgacatatt ttgcgccggc ttttgcagct aaaacagcct gatttggaga aaatatcaaa
    12001 gtaacattga ctggaattcc ttctgcggtt aaactcttac aagcctttag tccttctcct
    12061 gtcattggac atttaactac aacattgtca ccatacttac gaagtcttct tccttcttcc
    12121 atcattccat cgtaatcagt actaataact tcaagactta catcaccttt gataatttct
    12181 gaaatctctt ccatgatctt ttgtggatct ccaccctcct ttgctaataa tgaggggttt
    12241 gttgtaatac cgtcaacaag tcccatgtca ttatacattc gtataatctc aagattagct
    12301 gtgtctagaa aaattttcat gtttttttac tccttacttc ttttaccgct tttgctatat
    12361 taatagatgt caatccatgt ttttcaagca attgtggaat ctcgttatca cgtgctgatt
    12421 caccaaattt atcctgtgca ccaatacgtt ttattggcac tggataagat tctgaaacaa
    12481 cttcagaaac tgctgaacca aatccaccaa taatattatg ttcttcacat gttacaattg
    12541 atcctgtttc tctagcactt ttttctaata gatccttgtc aattggtttg atagaaaaac
    12601 aatctataac tctacaagat attccgtctt gttgaagcat attagctgca tccaatgcca
    12661 tcttcactgt aattccacat gcagcaattg tacaatcaga accatcacgt aatactatac
    12721 cctttccaat ctgaaattct tgcgagtcgt tatgaattat gggagttttt gatctagcca
    12781 ttctcatgta aaatggtcca tattgttgag atattatcca agtaagtttt gaaacagtag
    12841 ttgaatcagc tggtacaaga actctcatgt taggaatcac tcgcatgatt gcaatatctt
    12901 caatctgttg atgagaacca ccatcagctc caacacttaa accgccatga gataccacta
    12961 atttaacatt taatcccttt ttgtctccgg gagacggata tgcaatagca tttctgattt
    13021 gatcaacaca gcgccctggt agaaatattg catatgtact agcaaacgct gtctttccag
    13081 aatatgcaag acctgcagca actgaaacaa gattagcttc agcaattcct acattgaaga
    13141 atctctcagg gaattttttt ccaaagtttg aagtcttcaa tgaatcagtt gtatcagcac
    13201 ccaaaactac aacatttgga tcttgttcac caactgcaac aagtgcttta ctatattcag
    13261 ttctcatatc acctagatgt tctgtactca ttggaatcca agctcctttg caatctgaac
    13321 taaaccatct tttttctttc caacaacgtg taaatcaatc catttgttta caagatctgt
    13381 tccaccaaga tcatgagcct ttttgattaa taatcgtctt cttgaatcaa gaacttgatt
    13441 tctaaactct ttaatgatca ttctaacatc actttgtccg ataatagcac ttccacctac
    13501 aaatgctctt cctccatctg caaaacatgc accaatattt tctaaattta ctccgccatc
    13561 tacttcgata tatccatcaa acttatgatc atgaagatct ttagtaattc gctgtatttt
    13621 ttcatgcgta gattctataa atttctgacc ggatttacct ggaacaactg acatgactat
    13681 tatttggtct aacatttcag caaatttgta agaccattgt ggaagatctg tatctggatt
    13741 aattgcaaga ccaattccaa cttgattcga atgtaattga tcacatattt caccaaaact
    13801 agattcgtca catacttcag catgaactgt aattatatca cttccagcat ctatgtagtt
    13861 acgaacatgt ttcactggat cattgatcat aaggtgtgta tcaaaaggaa gtagtgatag
    13921 cggacgaagt ttctgaattt ctgtatgatc aaatgttttg ttaggtacaa actgaccatc
    13981 catcacatct agatgaatat aatcagctct tccctcgtta catcgttcaa cctcttttcc
    14041 aagatttgac atatcacctg caataattga aggtgcaatc ataaattgag attcaagctc
    14101 atcctcaata atgggaacaa tgtcttgttg aggagctttt ccatgccatt gcggattatc
    14161 ttccatatgt tctactcctt tgcctttgac ggttctagca ataattattg atggaaggcc
    14221 tttggtttca ttagctcttc ttattgcatc agaaatttgc tcagttctat gtccatcaac
    14281 ctctataaca ttccaaccaa aagaacgcca tttatcacca gtcttccatc tact
//
LOCUS       DASCPJ010000015         3000 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000200874, whole genome shotgun sequence.
ACCESSION   DASCPJ010000015 DASCPJ010000000
VERSION     DASCPJ010000015.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000200874"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="QGF01_00825"
     CDS             complement(<1..290)
                     /locus_tag="QGF01_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19482.1"
                     /translation="MSKQKLESNQEIIKNIIQTFPGLRFNEIKKEGSIANGTLQHHLS
                     ALQKAGSITVDYDNVTPRYFSSEINTDYQIIIKRLTQPTTSKIIKLLLKKEC"
     gene            414..974
                     /locus_tag="QGF01_00830"
     CDS             414..974
                     /locus_tag="QGF01_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19483.1"
                     /translation="METGALPKGFKSNQASANKTSANKAGIIITVSILAVALVGSGLF
                     LTGDFPMSETPESQMEPSTPVMQQEYDAPLSPEKSLSQPASYASSAGDLYGYLEFKIS
                     NGDIKSMTADDYNTSLVIAVDTYGDGEMYIDLDEQFIGSLDDGTYFVIVDNEEFDDYM
                     QAGTELYIPFEMGTEKIEIVGSYILS"
     gene            1071..1214
                     /locus_tag="QGF01_00835"
     CDS             1071..1214
                     /locus_tag="QGF01_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19484.1"
                     /translation="MDKPTAICNHGILHRKTDQNYQCLFCKDEFEVNIGLPVLNQEGE
                     MIT"
     gene            1298..1849
                     /locus_tag="QGF01_00840"
     CDS             1298..1849
                     /locus_tag="QGF01_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19485.1"
                     /translation="MTKLDANGFLESVGCAHVLYFHDGSKITKDTEFGFIKKGVEKQE
                     HCFYTTQDPEAILNQMKEFGIDTEASSKFLHLVKIPEKFEDYSKMILAKVDELPHSSQ
                     IRVVSTHYFDFNSEKRTDRMAEIEQCVEDDFHKLKGNFVCSFAVQKITNEIRGRFLNK
                     LLDSHRAIVIQTEKGGTEVFTLP"
     gene            complement(1846..2133)
                     /locus_tag="QGF01_00845"
     CDS             complement(1846..2133)
                     /locus_tag="QGF01_00845"
                     /inference="COORDINATES: protein motif:HMM:NF026297.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJN19486.1"
                     /translation="MEKNKVKERRTKTQIFFDVITAIIDDTQNNELVSPTRIQVKCNT
                     SYDKLTKYLEEMERREMIEKEKSITITEKGMEFHKDYSRINELINEMDKKF"
     gene            complement(2135..2602)
                     /locus_tag="QGF01_00850"
     CDS             complement(2135..2602)
                     /locus_tag="QGF01_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19487.1"
                     /translation="MYVPGFLQNRVYLLTIIAIITTISALGPFFFEDIACSVISTPTD
                     EHGHMSTETEGCWQLLAHETLHVAAITFGVFLFILSIIAYFSTRNTNMIFTAMAFATF
                     TFLSVFLLEEDLVAERIGHQESVVVDVLLTVMIGFFAIGVFSNQKFPTRRKYD"
     gene            complement(2660..>3000)
                     /locus_tag="QGF01_00855"
     CDS             complement(2660..>3000)
                     /locus_tag="QGF01_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-binding protein"
                     /protein_id="HJN19488.1"
                     /translation="TMGPIAAMLKEHETTKLIAEKIEEFAKKYLETGDSANLVDALDV
                     YVEHVSAHLWKENNRLFLMANARLQAYGKEVVDALQETETAKLAQLGKTRGDYEKMVD
                     DLKENLGAVQ"
BASE COUNT          969 a          515 c          558 g          958 t
ORIGIN      
        1 tggcattctt tttttaacag taattttatg attttagagg tagtgggttg ggtgagcctt
       61 ttaattataa tctgataatc agtattgatc tcgctggaaa aatatctagg agtgacatta
      121 tcataatcaa ctgtgattga acctgctttt tgaagcgcag agagatgatg ttgaagagtt
      181 ccatttgcaa tggaaccttc ctttttgatt tcattaaaac gaagccccgg aaaagtttgt
      241 attatatttt taataatttc ctggtttgac tcaagttttt gtttggacat atattatcta
      301 aaaaatcaaa cataggttat ttaaatttta attgaaaaat gtggtacaat aatcaaacaa
      361 aattaattct gataccggca ctcttatttt tacaatagta agttgcttta acaatggaga
      421 ctggtgcttt accaaaaggt ttcaaatcaa atcaagctag tgctaataaa actagtgcta
      481 ataaagctgg tattataatc acagtgagta ttcttgctgt ggctttagtt ggttctggtt
      541 tatttcttac aggagacttc ccaatgtcag aaacaccaga gagtcagatg gaaccaagca
      601 cgccggtaat gcaacaggaa tatgatgccc cattatcacc agaaaagagt ttgtcacaac
      661 ctgcatcata tgcatcttca gctggagact tgtatggtta tcttgaattc aagatcagta
      721 atggtgatat caaaagtatg actgctgatg actataatac ctctcttgta attgccgttg
      781 atacatatgg tgatggggag atgtatattg accttgatga acaattcata ggctcgttgg
      841 atgacggaac atactttgta attgtagaca atgaggagtt tgacgactat atgcaagctg
      901 gaacagaact gtacatccca tttgaaatgg gaactgaaaa aatcgaaatc gttggaagtt
      961 atatcctctc ataattcaaa aagtgtaaaa atcaagatga ccaaattatt cattcctaga
     1021 agacctaatc gttcagccca tactgatgat gaagaggagg agaattaagt atggataaac
     1081 caaccgcaat ttgtaaccac ggaatacttc atagaaaaac agaccagaat tatcagtgtc
     1141 ttttttgcaa ggatgagttt gaagtgaaca ttggattacc tgtattgaat caagaaggtg
     1201 agatgataac ataatgatca atcataacga ggaagaataa actcatcatg gtgagcgagt
     1261 attataagca attattcagt catacaaaaa gtataaaatg accaaattag acgcaaatgg
     1321 atttttagag agtgttggat gtgcgcatgt cttgtatttc catgacggta gtaaaataac
     1381 gaaagataca gagtttggtt ttataaaaaa aggggtagaa aagcaagagc actgctttta
     1441 caccacccag gatcctgaag ctatactgaa ccagatgaaa gaatttggta ttgatactga
     1501 agcaagcagc aaatttttgc acctggtaaa aattccagaa aagtttgagg attactccaa
     1561 aatgattctt gcgaaggtcg acgaattacc gcactcttct caaataagag tggtatcaac
     1621 ccattatttt gactttaatt cagagaaaag aacagaccga atggcagaaa ttgagcagtg
     1681 tgtagaggat gatttccata aacttaaggg aaattttgtt tgctcctttg cggtacagaa
     1741 gattactaat gagattcgcg gcaggtttct aaacaaacta cttgactctc atagggcaat
     1801 agtaattcaa acggaaaaag gcggaacaga agtttttacg cttccctaga attttttatc
     1861 catctcattt atgagctcat ttattctaga ataatcctta tgaaactcca ttcccttctc
     1921 agtaattgta attgattttt ccttttctat catttctctt ctctccattt cttccagata
     1981 ttttgttagt ttatcataag atgtgttaca cttgacttgg atcctggtcg gagatactaa
     2041 ttcattgttc tgagtgtcat ctattatagc ggtaataaca tcaaagaaaa tttgagtttt
     2101 agtccttctt tctttaacct tatttttctc catgctaatc atatttcctc ctagtaggga
     2161 acttttgatt cgagaaaaca ccaatagcaa aaaatccaat catcaccgta agtaaaacat
     2221 ccacaacaac tgattcttgg tgtcctatac gctcagctac taaatcctct tcgagaagaa
     2281 agacggaaag aaatgtgaat gttgcaaatg ccatagctgt aaaaatcata tttgtatttc
     2341 ttgttgagaa gtatgcaatt atgctaagta tgaaaagaaa cacaccaaaa gtaattgctg
     2401 caacatgaag agtttcatgc gctagtagtt gccaacaacc ttccgtttct gtggacatat
     2461 gcccatgttc gtctgtcggt gtggaaataa cggagcatgc aatatcttca aagaaaaatg
     2521 gaccaagtgc tgatatggtg gttataatag caataattgt aaggagatat actctatttt
     2581 gtagaaatcc tggtacgtac atttagaggt tacaaactgt cttcaataaa aatacaaatg
     2641 gtacaatggt caaagtgaac tattgtactg cgcccaaatt ttcttttagg tcatcaacca
     2701 tcttttcata gtcgcctctg gtttttccaa gttgagctag ctttgcagtt tccgtttctt
     2761 gtaaggcatc tacaacctcc tttccatatg cctgtaatct ggcatttgcc ataagaaata
     2821 atctgttgtt ttctttccag agatgggcac tcacatgttc cacatacaca tccaatgcat
     2881 ctacaagatt tgctgaatct ccagtttcca gatatttttt ggcaaactct tcaatctttt
     2941 cggcaatcag ttttgtggtt tcatgctctt ttaacatcgc agcaattggt cccatggttg
//
LOCUS       DASCPJ010000016         6393 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000239071, whole genome shotgun sequence.
ACCESSION   DASCPJ010000016 DASCPJ010000000
VERSION     DASCPJ010000016.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000239071"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(178..462)
                     /locus_tag="QGF01_00860"
     CDS             complement(178..462)
                     /locus_tag="QGF01_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19489.1"
                     /translation="MSHDEQIQMMINQTMDSAISQIEGYLGEIERSNDILKISDSKEF
                     VYGLIIGQTLGLAMAALSSLKKEMPTQNDQEKIRDMIYKNVPELRKRLFE"
     gene            complement(507..1073)
                     /locus_tag="QGF01_00865"
     CDS             complement(507..1073)
                     /locus_tag="QGF01_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024724.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJN19490.1"
                     /translation="MIKSNNSLINESKDIAKKAHKNQKRKDKKTPYINHLRMVVDLIK
                     KSGVTGNDVQIIGWLHDTIEDTPTDFDDIKNQFGLHIAKCVSALTKDTRVEEQKRDRL
                     YVRELKKAHWHVKLVKICDITANILDLKNTEYSEKEKINHWKRKKPYLIAIKYGLIKN
                     KKKIPHLKIIFDNLNCGLAKFGQSPVSI"
     gene            complement(1070..1423)
                     /locus_tag="QGF01_00870"
     CDS             complement(1070..1423)
                     /locus_tag="QGF01_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HJN19491.1"
                     /translation="MQTKLGVEFEIDFAHTLKDHPKCGKPHGHTAKIIIEVAGDVKKG
                     DTYQDNMVMDFHEMKTTCWDVIKDLDHTNLNDMFDFPTSENIAMWIFDNLKERIPISS
                     VKFFEGSNKYCEITK"
     gene            complement(1525..2886)
                     /locus_tag="QGF01_00875"
     CDS             complement(1525..2886)
                     /locus_tag="QGF01_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJN19492.1"
                     /translation="MNDTSSSEIKREFMKSKSGLVGIGILACLIIMSIIAVITIPIDT
                     FKQWNNPGNWISYPKTSIPIWVNYFTTEKIPEHVIIENPEIITKDGIISLTSQQFMLD
                     YDYSDFPDGMIYEFDTEYSGSVLLHISVIRPDQTEMLLLSTSLPYSDTKTQHHERIFT
                     TDNNIKKNLEFQSANLDANKNTKSSEMLIFSDANGEVLNGRYLFLVNFYGVNQQLEIL
                     DSKLIVGGKAFGLMGTDELRRDLMVGLLWGTPLALFIGIVVAIGSVTIGLLYGVYAGF
                     KGKKTDEGMMRFNDIIYALPALPFLIILAVTISNSIFVIIGFLLIFSWVGIAKVSRSM
                     TLQIKIKQYVEASKIMGQTDSKIISKHIIPQLLPYAFASIAISVPAAITTEAGLSFLG
                     LGDPTFPTWGQILHDAHTFGAASRGLWWWIIPPGAMVAITGLAFVFIGNALDAIINPK
                     LKR"
     gene            complement(2883..3896)
                     /locus_tag="QGF01_00880"
     CDS             complement(2883..3896)
                     /locus_tag="QGF01_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJN19493.1"
                     /translation="MFGVLMVTLLLTIALVGSNMDVILKQGIIYQVRSEITENSSIVE
                     SFDSVKEFEEFVQVQIDQRIKALGLDNPWYSPQRIGFTMYKILILDFGNATFLTSDSG
                     SSNVGEILLEKIPRTVLLFTTATIIISMIGIFLGALSGSKVGSVVDRLTSTFAVISSS
                     FPVWWIGMLMIFLFAFTYHIFPARATPSMLPSEPGYVIALLYHMALPLITIVIIGFGS
                     WAYLVRNFMVGTMQEDFITAKKAIGIKKNKIIYRHALKNAAPPIVTILALSLSGSLGG
                     AIITEAVFDWPGMGRLYFEAISVMDLPIIIGATYVLTVLFLISIFIADLLYGYFDPRV
                     KRE"
     gene            4224..5936
                     /locus_tag="QGF01_00885"
     CDS             4224..5936
                     /locus_tag="QGF01_00885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJN19494.1"
                     /translation="MKNIGTLEYVLDKYSGTWTWKITGDRAVFMVSKIIPKVWYGDGP
                     NEVIVPDDESTVKQIKMILERYPMEILSKSVWQRKSSPVLIKKQKPVKIEKLSKAKPG
                     KQFRGNLLNFQKEGLDFLLKSTGNALLADDMGLGKTVQTLAYIASEKQSLPVLVVAPL
                     VTLKNWQREIIRFMKKRSKNGRIIEDDAPTTTLIRTGKNEPLGDFDFYIINYELLKKR
                     LDDISRLKIRTVICDEVQHLRSKTTQKYTAIKKIAGLDIVNYRIGLSGTPIYNHGSEI
                     WPIVDILKPGFLGSFKEFCEYFCYMNEKGRAIVLENKRESLRHELQKHIMLRRKKSDV
                     LKELKDKVRHKEYVDADVDYYKNELNKIWHKLEQEQQNAETEFAKSASYQRAIQSERQ
                     AAGIAKLHHVVDFVKNTMEIEESVVVFCHHKMIHTLLHEKLGEFNPASIIGGQTDNVR
                     QNNIDNFQEGKTKLMIAGLRAGNVGINLTTAKYVIFAELDWSPAIHRQAEDRLHRIGQ
                     KNTVFAYYLIGNGTLDDHVGDILVDKEYEIDTIMDAGTESFENKEKSKLILAQIHDKI
                     RTKN"
     gene            complement(5969..6112)
                     /locus_tag="QGF01_00890"
     CDS             complement(5969..6112)
                     /locus_tag="QGF01_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HJN19495.1"
                     /translation="MTTGYCVKCRSKREIKGATAVTMKNGKPATKGTCPTCTTTMFRI
                     GKA"
BASE COUNT         2183 a         1073 c          994 g         2143 t
ORIGIN      
        1 gtattatata tcattcacac tgcattttga acagtataat tatttagaat ctgataattg
       61 cgcattaaag agatttcaat actgaggata aataaattat tctataaatt ttctatagcc
      121 tcactttgct ttttcacaag gatatgagag cactttattt tatttgccat ttcaacacta
      181 ttcgaaaagc cttttcctta attcaggcac atttttgtat atcatatcac ggattttttc
      241 ttggtcattt tgggttggca tttctttttt taatgatgat aatgctgcca tggctaaacc
      301 caatgtttgt ccaattatta atccataaac aaattcttta gaatctgaaa ttttaagaat
      361 gtcattactt ctttcaattt ctcccaagta tccctcaatt tgtgatatcg cgctatccat
      421 tgtttgattt atcatcattt gtatttgctc atcatgactc atgattggtg aaacaaataa
      481 catgtaataa atatacagtt caagttctat atactgactg gactttgtcc aaattttgct
      541 aatccgcaat ttagattatc aaaaataatt tttagatgag gtattttttt cttatttttt
      601 attaatccat attttattgc aataagatat ggcttcttac gtttccaatg attaattttt
      661 tctttctctg aatattctgt gttttttaga tcaagaatat tggcagttat atcacagatc
      721 tttacaagtt ttacatgcca atgtgctttt tttaattctc ttacgtaaag tctatctctc
      781 ttttgctctt caaccctcgt atctttagtt aatgcagaaa cacatttggc aatatgcaaa
      841 ccaaattgat tcttgatgtc atcaaaatcg gtcggagtat cttcaatcgt atcatgcaac
      901 caccctataa tttgtacatc attaccagta acaccacttt ttttgatcaa gtcaactacc
      961 attcgcaggt gattaatgta gggagttttc ttatcttttc ttttttgatt tttatgtgct
     1021 ttttttgcaa tatcttttga ttcatttatt agagaattat tgctcttaat cattttgtga
     1081 tttcacagta tttgttacta ccctcaaaaa attttacact agaaatggga attctttctt
     1141 ttagattatc aaaaatccac attgcaatat tttctgacgt aggaaaatca aacatatcat
     1201 taaggttagt atggtccaaa tctttgatca catcccaaca cgtagttttc atttcgtgaa
     1261 aatccatcac catattgtct tggtatgtat cacccttttt cacatcacct gcaacttcaa
     1321 ttatgatttt ggcagtgtga ccatgcggtt taccacattt tggatgatct tttagtgtat
     1381 gtgcaaaatc tatctcaaat tcaacgccaa gttttgtttg catattccct cattgtatat
     1441 gggtttcaaa tacttagcgt atatccacga ataaattcta gatataaaca caaaatttct
     1501 cttaaattga ttctattttc aaaattaacg tttcaatttt ggatttatta tagcatctag
     1561 tgcattccca ataaatacaa atgctaatcc cgtgattgca accatagcac caggtggaat
     1621 tatccaccac cataaacccc tagacgctgc accaaaagta tgtgcgtcat gtaaaatttg
     1681 cccccatgta ggaaatgttg ggtcaccaag acctagaaaa cttaaaccag cttctgtcgt
     1741 gattgcagca ggaactgaaa ttgcaatact agcaaaagca tatggaagta gttggggaat
     1801 aatatgtttg gaaattattt tagaatctgt ttgccccata atttttgatg cctcaacata
     1861 ttgttttatt tttatttgga gtgtcatact acgtgatact tttgcaattc caacccaact
     1921 gaatatcaat aaaaatccaa ttatcacaaa tatactgtta ctgatcgtca cagcgagtat
     1981 aatcaaaaac ggtagtgcag ggagagcata aataatatca ttgaatctca tcatgccttc
     2041 atcagttttt ttccctttga aaccagcata caccccatag agtaatccta ttgtgaccga
     2101 gcctatagca actacaatgc caatgaataa tgccaatggg gttccccaca aaagaccaac
     2161 cataagatca cgtcgtaatt catccgttcc catcaatcca aatgcttttc caccaacaat
     2221 taatttagaa tcaagaattt ccagttgctg attcactcca taaaaattta ccaaaaacaa
     2281 atatcttcca tttagaactt caccattcgc atcagaaaag atcagcatct cactggattt
     2341 agtatttttg ttagcatcta aatttgctga ttgaaattca agattttttt tgatattgtt
     2401 atcggtagtg aaaatccgtt catgatgttg agtttttgta tctgagtatg gtaatgaagt
     2461 agataaaagt agcatttcag tttggtctgg tctaataaca gagatatgaa gtagtactga
     2521 tccagaatat tcagtatcaa attcatagat cattccatct gggaaatcac tataatcata
     2581 atctagcata aattgctgtg atgtcaatga tattattcca tctttagtga ttatttccgg
     2641 gttttctatt atgacatgtt ctggtatttt ttcagtagta aaataattca cccatattgg
     2701 aattgatgtt tttgggtatg aaatccaatt tccaggatta ttccattgtt tgaatgtatc
     2761 aattggtatt gtgataactg ctataattga cataataata agacaagcca aaatcccaat
     2821 acccacaagc ccgcttttac ttttcatgaa ttctcgtttg atttctgatg aagatgtatc
     2881 attcattctc ttttcaccct tggatcaaaa taaccataaa gtaaatcagc gataaatata
     2941 ctaattaaaa ataaaacagt gaggacatat gttgcaccaa taataattgg taaatccatg
     3001 acactgattg cttcaaagta aagtcttccc ataccaggcc aatcaaaaac agcttctgta
     3061 attattgcac cacccaatga gcctgaaagg ctgagtgcga gaattgttac tattggtgga
     3121 gctgcgtttt ttaatgcatg tctatagatg attttattct tttttatacc aatagccttt
     3181 tttgccgtga taaaatcttc ttgcattgta ccaaccataa aattacgtac taggtatgcc
     3241 caagagccaa atccaataat tactatggta attaatggta atgccatgtg ataaagtaat
     3301 gcgatgacat atcctggttc acttggaagc atagacggtg tagctcttgc tggaaaaata
     3361 tgatatgtaa acgcaaataa gaaaatcatt aacatgccta tccaccatac aggaaaacta
     3421 ctactaatta ctgcaaatgt ggaagtaagt ctatcaacta ctgaacctac tttactgcct
     3481 gataatgcac ctaaaaatat accaatcatt gatatgataa ttgtggcagt agtaaagagt
     3541 aaaactgtac gcggaatctt ttctaataat atttctccaa catttgaaga accagagtca
     3601 cttgtaagga atgtagcatt tccaaaatca agaattagta tcttatacat tgtaaagcca
     3661 atcctttgtg gagaatacca tggattatct agcccaagtg cttttattcg ctgatcaatc
     3721 tgtacctgga caaactcttc aaattctttt acactatcaa aactttctac aatagaactg
     3781 ttctcagtaa tctctgatct tacttgatag attattcctt gtttcaaaat aacatccatg
     3841 ttagagccaa ccaatgctat agtcagcaat agtgtaacca ttagtacacc aaacattgtc
     3901 agacctcttg tagcaatgta tctttttaga ctcaataatc aggtctagaa aaattacctt
     3961 ataacttatt tcagttaaaa ttttaattct acacactatt taataatata gagtagatct
     4021 aaaattagta tgcgatgtgc gagttgtgag aatcataaac attatgaatg tattgattgt
     4081 cagaatgatc ataaaacaca tctttgtaat tgtgattgtc atactacaga gcacaaacac
     4141 gagtaagttg atccattaat acttccagca aaaaacaaga ttcataaacc attaaatcaa
     4201 tttagagaaa tcacgaatct atcatgaaaa acattggaac attagaatac gtattagaca
     4261 aatattcagg aacatggacc tggaaaatca ctggcgatag ggctgttttc atggtatcaa
     4321 aaataattcc taaagtatgg tatggcgatg gtcctaacga agtcattgtt ccagatgacg
     4381 aaagtactgt gaaacaaatt aaaatgattc tagaacgata tcctatggag atactctcaa
     4441 aatctgtatg gcagagaaaa tcatcaccag ttttaattaa aaaacaaaaa ccagtaaaaa
     4501 ttgaaaaact ttcaaaggct aaacctggga aacagtttcg tggaaattta ttaaatttcc
     4561 aaaaggaggg attagacttt cttttaaaat ccactggtaa tgcattacta gcggatgaca
     4621 tgggattagg taaaactgtt cagacacttg catacattgc atcagaaaag caatcactac
     4681 ctgttcttgt agttgcacca cttgtcacat taaaaaattg gcagcgtgaa attataagat
     4741 tcatgaaaaa acgaagtaag aatggaagaa taattgagga tgatgctcct actaccacac
     4801 ttatccgtac tggaaaaaat gaaccgcttg gagattttga tttttatatt atcaattatg
     4861 aattattgaa aaagagatta gatgatatat caagactaaa aattagaact gtcatatgtg
     4921 atgaagtaca acatttacgt tctaaaacaa cacaaaagta tacagctata aaaaaaatag
     4981 ctgggttaga catagtgaat tacagaatag gtctttctgg aacaccaatc tacaatcatg
     5041 gttctgaaat atggcctata gttgatattt tgaaaccagg atttttgggt agtttcaagg
     5101 aattttgtga atatttttgt tatatgaatg aaaaaggacg tgcaattgtg cttgaaaata
     5161 aacgagagag tttacgacat gaattacaaa aacatatcat gcttcgacga aaaaaatcag
     5221 atgtattaaa agaattaaag gataaagtga gacataaaga atatgttgac gcagatgttg
     5281 attactataa aaatgaatta aataaaattt ggcataaatt agagcaagag caacaaaatg
     5341 ctgaaacaga atttgccaaa tctgcttcat atcaacgagc aattcagagt gaacgacagg
     5401 ctgcgggaat tgcaaaactt catcatgtag ttgattttgt aaaaaatacg atggagattg
     5461 aagaaagcgt tgtagttttc tgtcatcata aaatgataca tacgttactg catgaaaaat
     5521 taggagagtt taaccccgca tctataattg gaggtcaaac agataatgta agacaaaata
     5581 atatagataa ttttcaagaa ggaaaaacaa aattgatgat tgcaggctta cgcgctggta
     5641 atgtaggaat aaacctaaca actgctaaat atgttatatt tgccgaatta gattggagtc
     5701 ctgcaattca ccgtcaggct gaagatcgtt tacatagaat agggcaaaaa aatactgttt
     5761 ttgcttatta tttaatcggt aatggtacac ttgatgatca tgttggagat atcttggttg
     5821 ataaggagta tgagattgac acaattatgg atgcaggaac tgaatcattt gagaataaag
     5881 aaaaatcaaa actcattctt gcacaaattc atgataagat acgcacaaaa aactagagga
     5941 ataaataata aaaaagtgag aaatgttcct aagctttacc tatacggaac atcgttgtag
     6001 tgcaagttgg acaggtacct tttgttgctg gtttaccatt tttcatagta acagcagtgg
     6061 cacctttgat ctctctctta cttctacatt tcacacagta acctgttgtc atggtaagat
     6121 tgagtattat ctcatattag caaggacttg tgaactagat tttacatttt tgtgactctg
     6181 ttttatacca aaatcatcga agaatacaca aactttgcca gttttccctt taaaacctag
     6241 ttttttaaaa ataatcaaat aaaactagga atattatcac agatatcaat ggattataaa
     6301 cactatttca ttatttttat gatgttttta gcatttttac cattggatgt ttttgctgat
     6361 tcatctatcg atatctcatt agatcttccg ata
//
LOCUS       DASCPJ010000017        46220 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000243537, whole genome shotgun sequence.
ACCESSION   DASCPJ010000017 DASCPJ010000000
VERSION     DASCPJ010000017.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 46220)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46220)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46220
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000243537"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="QGF01_00895"
     CDS             <1..557
                     /locus_tag="QGF01_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HJN19496.1"
                     /translation="EAANKIGYSVVMKIASPQIVHKSDAGGVKVNLTNDDEVRNAFKE
                     IVENAKKYDSDAEIKGVLIVEMVKGGKEMIIGSKLEPGFGPVIMLGMGGIYVEILKDV
                     TFRLAPLTDQESNDMIMSIKTKKLLDGVRGEKPSDVEKLSECIQRLSQLVTDFSEIKE
                     LDMNPVLVMEKNNGCKILDVRIGI"
     gene            621..2147
                     /locus_tag="QGF01_00900"
     CDS             621..2147
                     /locus_tag="QGF01_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HJN19497.1"
                     /translation="MQKTIRPIRTGEEYIESLRGRDLTIYLFGELVKEPVDHPMIRPS
                     INAVAKTYDLAVEEEDLASVKSSITGEQVNRFLHIAESAQDVVKQNKMQRKLGQLTGT
                     CFQRCVGMDALNSLHSTTFEIDEKHGTDYHKKLLEFIKMVQHENLVIGGAMTDVKGDR
                     SMGPSDQEDPDLFLRIVERNDKGVYVSGAKAHQTGCINSHWMLVMPTMRLTENDKDYA
                     VVGAIPVDAPGITYIYGRQSCDTRSMEPGDIDQGNSEYGGQEAMVIFDKAFIPNEMIF
                     MDGEYEFASMLVERFTCYHRRSYVCKTGLGDVLIGAAATIADYNGVPKVSHIKDKLIE
                     MTHLNESIYGTGIASSYQSQKMKSGVWQNDDMLANVCKHNVTRFPYQISRFAQDIAGG
                     LMVTLPSEAELRNPITGPLLEKYLKGRKGVDVESRMRILRLIENMTMGRNAVGYLTES
                     MHGAGSPQAQRIQIARQMQLGYKKRLAKNLAKIQDDGEETLENADYFKRVFKLDNSKE
                     "
     gene            complement(2150..2860)
                     /locus_tag="QGF01_00905"
     CDS             complement(2150..2860)
                     /locus_tag="QGF01_00905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HJN19498.1"
                     /translation="MGSSSIKSIVKDIIIVAICVAVIWIGLQAYFGAVNPFYVVSSGS
                     MYPQLAMYDIIVITGHTPFEDVKIGDIIVFDRPKDHDKVIVHRVVAVVDDDPKTLRTK
                     GDNNQASIPGTDFPITEQEYVGTVVHVIPQVGYITKILQPPINYIIIAVIIGIITIRE
                     IAKKYNKKDLIINDTDESKSDEKIDYFLNDKEYSSNDFLANDGKSTDGISKEDTKSID
                     DIPEFFRDKEQKSEEEKE"
     gene            complement(2908..3714)
                     /locus_tag="QGF01_00910"
     CDS             complement(2908..3714)
                     /locus_tag="QGF01_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19499.1"
                     /translation="MSSDLRIKKLRGSGGYVMAQVSDEQQRKGNLGGPDLFLAPIGRL
                     DEYTIKKYSCNICDKEYDGSPKIEYEHPNEQVADNLILVERGQYLCIECDSPIAEYRE
                     FQKSDESSDIGNAKPTEVNAEIFQELNNDTQEDSESIMNEIISENNEQFENVDKSLET
                     DVESGDIDSTFSAITGKIVFDENAKQIGVAKQVGVNSKNEVVLVMTDNYGNDKSVDWQ
                     KIKKIGEIVLLGNTVTERENIPAPDGLSCSNCNFDNKSDAKFCENCGSKV"
     gene            3805..4131
                     /gene="trxA"
                     /locus_tag="QGF01_00915"
     CDS             3805..4131
                     /gene="trxA"
                     /locus_tag="QGF01_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HJN19500.1"
                     /translation="MAVVHVSDTKTWETDVIGSSTPVFVDFWAEWCGPCRMVGPVVEE
                     LSGDYDGTVKFVKVNVDEANELASKYNVFSIPTLMIFNNGQVVAQQVGAASKETYKTM
                     IDKALE"
     gene            4197..4736
                     /locus_tag="QGF01_00920"
     CDS             4197..4736
                     /locus_tag="QGF01_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HJN19501.1"
                     /translation="MSLGEVDTLNLLTDKLNNLFEESQGYYESFLDTNNMYKEGKLTE
                     REFFQKLGDYVVAYSALEFLSIKVIFEIKKAVDKISGGTSLGGTQSPGLMPGMGTPGM
                     MPGGMTTPPGPRSGTADNPVGGPASVISAATAFDNAGTLPTPDPSLVSKPSNAAKCSS
                     CGSDLRPNAKFCTNCGNKI"
     gene            complement(4750..5112)
                     /locus_tag="QGF01_00925"
     CDS             complement(4750..5112)
                     /locus_tag="QGF01_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJN19502.1"
                     /translation="MKVFNGKQAAEDYMAAHTLTFSTPDLTLKRFSFWLGDIVPDPNN
                     KEETMPRLMTYIEEKDFAPTQVIDDDSYEPSGAVLKSGLYDSTNTDSGSQTKFCSECG
                     EKISASARFCTECGAGQE"
     gene            complement(5152..5223)
                     /locus_tag="QGF01_00930"
     tRNA            complement(5152..5223)
                     /locus_tag="QGF01_00930"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5189..5191),aa:Ala,seq:cgc)
     gene            5298..6695
                     /locus_tag="QGF01_00935"
     CDS             5298..6695
                     /locus_tag="QGF01_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJN19503.1"
                     /translation="MTYDTLIVGSHIITPTGIIDKNMILDEGKIVKLTNETPSCDHRI
                     NAEGLVSIPGVIDPHVHYGVYSPIEQAAQSESHAAAIGGVTTMMRMLRLGDSYEKSLS
                     DQLTSSSNSHYVDYAIHASIFTLNQVNEMRFCVESGVTSFKLYMNLGGEVGHVYMDMK
                     PGENVLREECVEVNSEIITKVVQNAASLGCPVLVHAEDYEQCGCGIKKSKEKNLDGLS
                     AWSKSRPSESESHSIKTISKLARDFNCVLYFAHIGSRTALQQINEERKNGTKIFTETC
                     PHYLTLSYEKQEGYLAKVMPPIRSDDDVLAVWNAINENQVDTIGTDHVANQLKLKLDG
                     DTIWDALAGFPGIGTSLPLLLSEGVNKNRITLQQLVNITSTNTAKIFGMHPTKGSFEP
                     DSDADITLIDLNKEQKVTNDLFGGFSDYLIYEDWKLKGWPVKTIVRGQTVSEDFQVVG
                     KCGYGKLVPRKNNSI"
     gene            complement(6688..7362)
                     /gene="rpiA"
                     /locus_tag="QGF01_00940"
     CDS             complement(6688..7362)
                     /gene="rpiA"
                     /locus_tag="QGF01_00940"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HJN19504.1"
                     /translation="MTYDSAIKALSQDALKFVKNNQVIGLGSGRAATAFVKSLSSYVK
                     KNKIHVKCIPTSLQIKLEAEKGKLHLMDTVQDQIDVVFDGADQIDKQKNLIKGGGGAL
                     LRENILISAAKKVVIMADDSKFVTNFNRDVPVEVHPLARNTATKDILKIGGKPSIRTL
                     DRGYPFVTENGNIILDCDFKTIKKPKALQEKIQSIPGVLEVGIFTRKPDIIYKALKDG
                     RFDVIK"
     gene            complement(7363..8259)
                     /locus_tag="QGF01_00945"
     CDS             complement(7363..8259)
                     /locus_tag="QGF01_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19505.1"
                     /translation="MQEINYSIKNESNSLVIERDILTKSISRLYQQDPQLTRIQQDKL
                     LTKYQYQLRVVNEKIKNLESKKEPDVLSSKDQSYKEMDQKLSRIEKKLQELTIKFTRP
                     NEDVYKANQKPTQKPKKVDSQILAKKNEDLNLYTSNMKIDSKSQKDLEVITMTEVPKK
                     IPEFFLTKFKEKQLETKTIQHSTDIPVVKNLENVVKIAVPETNVCERPECTNPKFTNR
                     HCTLHSNSNQTKFENNETEVIIPSSQAVESQQTEIKPPITNGNTKEQVKDVDESLDED
                     DITKVVASIEKSLSNLDQVEVE"
     gene            complement(8260..8427)
                     /locus_tag="QGF01_00950"
     CDS             complement(8260..8427)
                     /locus_tag="QGF01_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HJN19506.1"
                     /translation="MVKGTTSMGGFTRKHVHIRCRRCGKNSYHKRHHTCASCGFPDSK
                     IRKYSWIKWYT"
     gene            complement(8441..8671)
                     /locus_tag="QGF01_00955"
     CDS             complement(8441..8671)
                     /locus_tag="QGF01_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LSm family protein"
                     /protein_id="HJN19507.1"
                     /translation="MSVDMAVKILDESINKTVLIKLKGSKTIRGNLLGFDQHMNLLLD
                     QSEEIPSEGDSKELGTIVVRGDNVIMISPPPS"
     gene            complement(8750..9457)
                     /locus_tag="QGF01_00960"
     CDS             complement(8750..9457)
                     /locus_tag="QGF01_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HJN19508.1"
                     /translation="MDASDLYDPEFRKLIKSKKPTVIIPVGSIEQHGAHLPITTDSDI
                     VTEVASRLAKKCGFIVFPTISYGVSKEHDPFFNVSLESNTLWNIIGDIDRSVVKNKLK
                     SVIILNGHHGNALSLKLAERKTILQRPARMRKIRFYSYWHFMNHEFDHAGFVETSIML
                     AISNKVKMKKAKKGLITKGLSEKEIQRISKLSTQPGGFPQVTKNGVWGDPTGATKKDG
                     QKFLTEIVRNLAKECQS"
     gene            complement(9463..10305)
                     /locus_tag="QGF01_00965"
     CDS             complement(9463..10305)
                     /locus_tag="QGF01_00965"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HJN19509.1"
                     /translation="MKKTIVGITVVGKDREGIVAQFTNFVFQKHGNIEKVNQNVIKGL
                     FGMYLEVSFTSKINFKKLDSELQKLGKKIRMEVSTHSETNSEKNIAIFVTKEDHCLKE
                     ILSARRTVKGKISVIVGTEKALAPLAKKAKIPFIIVNDRNQEKAEEKLLKLCKKFEVD
                     VIVLARYMRILTPNFVWRYPDRIINIHPSLLPAFPGSLAYAQAFERGTKIVGVTSHYV
                     TENLDQGPIIFQDSFKVTPEDTLESIKRNGQKLEATTLFKAVKMHLDNKLEVRWRKVH
                     VKSK"
     gene            complement(10363..10450)
                     /locus_tag="QGF01_00970"
     tRNA            complement(10363..10450)
                     /locus_tag="QGF01_00970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10412..10414),aa:Leu,seq:tag)
     gene            10498..11865
                     /gene="glmM"
                     /locus_tag="QGF01_00975"
     CDS             10498..11865
                     /gene="glmM"
                     /locus_tag="QGF01_00975"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HJN19510.1"
                     /translation="MGKLFGTNGVRGIFSEDFNLEFVHDLVLSISAYFKHGTILVGYD
                     GRHSSPTISKTVCDTLNSAGLDCHLAGLVPTPCLEYATKKLGYDGGIMITASHNPPEY
                     NGIKPVASDGVEISRDDEKSIEEIFFQKKWMENNSALGYTTNDSRAIQTYLDGIKFQV
                     DVDKIKSKKFKVVLDLGNGAQIITAGKLCDQLGCEVIMINEQIDGNFSGRGSEPTPYN
                     LEELSSTVLNNNADIGIAFDGDGDRSIFCDNEGKLLSGDKSALLLTKYLLNKNPNSKI
                     VTCLNSGNSIDEIVTNTNSSVIRTKVGSVEVSRRMIVENALLGFEENGGFMFGKHNHV
                     RDGAMTLALVLDLLADSGKTITEELQILPSSFTTKDKISCTNEAANRVISELKSMFPD
                     ADITDGIKIVFDKKNWIMVRQSGTEPIMRIYGESDSQKNLEVLMRTYLDKIQLILSDN
                     TFKTT"
     gene            11907..12293
                     /gene="rpl7ae"
                     /locus_tag="QGF01_00980"
     CDS             11907..12293
                     /gene="rpl7ae"
                     /locus_tag="QGF01_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HJN19511.1"
                     /translation="MTKPYYVKFETPENLVSPILESLRVAAETGKVRKGTNEATKAIE
                     RGISKLVIIAEDVEPPEVVAHLPIICEEQKAHYAFVPSKQDLGSALGIEVTAAAAVIL
                     DAGDAQHIIDEVIASIDKIKGGKSDE"
     gene            12290..12508
                     /locus_tag="QGF01_00985"
     CDS             12290..12508
                     /locus_tag="QGF01_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HJN19512.1"
                     /translation="MSSTEHSDELTTAEVIQIVGRTGIAGEVIQVRVKILDGKDRGRI
                     LTRNVKGSIRMSDILILRETEREAKKIR"
     gene            12518..12721
                     /locus_tag="QGF01_00990"
     CDS             12518..12721
                     /locus_tag="QGF01_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HJN19513.1"
                     /translation="MSMLVKSCSFCDRPVAKGSGTMFVKNDGTVMWFCSAKCKKNSLV
                     LKRDPRKLKWTKKYVKGGIRVKK"
     gene            12723..13121
                     /gene="ndk"
                     /locus_tag="QGF01_00995"
     CDS             12723..13121
                     /gene="ndk"
                     /locus_tag="QGF01_00995"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HJN19514.1"
                     /translation="MAEQTLIIIKPDAAKRNLVGEILSRFEKKGFTISKIKALNFTVE
                     MAEQFYSAHNSKPFFGELVSFITSRKVVAAVIEGENVIDTTREIIGKTNPKDAAPGTI
                     RGDFGIDITENSIHASDSSESFDKEVNVVF"
     gene            13131..14912
                     /gene="infB"
                     /locus_tag="QGF01_01000"
                     /pseudo
     CDS             13131..14912
                     /gene="infB"
                     /locus_tag="QGF01_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
     gene            complement(14918..15334)
                     /locus_tag="QGF01_01005"
     CDS             complement(14918..15334)
                     /locus_tag="QGF01_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HJN19515.1"
                     /translation="MSYKYLEHATDAFIEVKAKTMEEAFLVAGKSVVETIIDSKNIQE
                     IEEKYIKVVGENIHNLLYNWLEEIVTITITDGFAIKNFSVNIKKNKEYQIVSKISGEK
                     LNLKKHNYKVEIKSPTFHLMEIKENDGVLMRYLLDL"
     gene            15415..16689
                     /gene="hisS"
                     /locus_tag="QGF01_01010"
     CDS             15415..16689
                     /gene="hisS"
                     /locus_tag="QGF01_01010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HJN19516.1"
                     /translation="MELPRGMKDFDKNEMIKIEFIRQKFLETASVFGFNLMEPSPIEL
                     LSVLEAKSGPSIRDEIYQFQDKGDREVALRFDFTVGLTRYATSQKTLKLPAKLSSFGG
                     VWRYDEPQKGRYRFFHQWNIEIFGNLNSDYDAEIIEFTSRFFDNLTMKNIIIDINHRK
                     LVQSYINQLFESNETSLLNDIFRAVDKIQKKSKNEILQEYTKKGYSSEKLEKILEFSK
                     IKGTPSEIQQNFDVSDLDGWDELVTLHDSLKHRGIDNIRINFGIVRGLDYYSGIVFEA
                     YDTTSDLGALVGGGRYDSLPNTFGRNDFGATGVAGGVERIILCLDAQNVSYTQSHYTV
                     SVLYSNEELKLEAVRTASKLRNLGIPTNIDLNGKSLKKQMEISSSSRFSIIFAPQEFS
                     EKQVVLRNMVDRTEKRISLEELITNPKDILNL"
     gene            complement(16679..17344)
                     /locus_tag="QGF01_01015"
     CDS             complement(16679..17344)
                     /locus_tag="QGF01_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HJN19517.1"
                     /translation="MDIIKYDVYSGPNIGIYTKVNNQFVFVPNGFAKTKAENLANYLQ
                     TDYLTISIANTRLLGILAVLNNHGMLLPNTCSQEEIDHLKKHTDLNIKLLDIKYNALG
                     NLICANDKGGIVSPTIDKKYVKEIEDVLDIEVLQKKIAGFYQVGAVMSATSNGGIIHP
                     LTDEDDVNTFSNILGVKIEPATINGGIPFVSSGILANDNAIVVGTLTNGPEIMMLTSA
                     FIN"
     gene            17470..18024
                     /locus_tag="QGF01_01020"
     CDS             17470..18024
                     /locus_tag="QGF01_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HJN19518.1"
                     /translation="MPIKEDFCKGDKKPIGKINYSDGENFHWVWPSQAGEAGNDWDAS
                     TDDKVLADYKKHGEKMEKLGITGTMVANDWDVCVADGACIEACPVQIFQWYRTDKDIS
                     GIDAVKDKTPWPGAGTTEKEERLDFTDKADAIREHDCIWCMACVSVCPPLAVLVDQGN
                     QEFHEKAAGTFQELGSGQANPHSH"
     gene            18092..18175
                     /locus_tag="QGF01_01025"
     tRNA            18092..18175
                     /locus_tag="QGF01_01025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18126..18128,aa:Ser,seq:tga)
     gene            complement(18176..19132)
                     /locus_tag="QGF01_01030"
     CDS             complement(18176..19132)
                     /locus_tag="QGF01_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HJN19519.1"
                     /translation="MQSNVMWTEKYRPQKISELINQKETIGSLNSMLKNQLELPHLLF
                     SGSAGVGKTTISLCICKEILGEYWKNYTLELNASDERGINMVRERVKKFSRFAGLDTK
                     IPFKIIILDEADEMTSDAQTALRRIIEDTAKFCRFILIANNLSKIIEPIQSRCVVFKF
                     TKVSEKDILLHLKQIGKNEKIKIDEKGLGAISAYADGDLRHAINILQASASTGNVTVD
                     SVKSVIGLTKTNDVQNILKMAIDGKISESREKMIELIKVYGMSESDFLKYINQAIFNL
                     KNDNIAEMSEITAKYDYRILAGANPEIQLSALLAELTKFSKK"
     gene            19253..19771
                     /locus_tag="QGF01_01035"
     CDS             19253..19771
                     /locus_tag="QGF01_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19520.1"
                     /translation="MLDIQNLVRVHSIGNDLHDVKVEFNKDLKIDISDITVEGKQGEI
                     INIPRWIADILHSAKHVEIKDEDMFSELKQSIVKEKVQGEFDLSTLDDYFYIKLKSYM
                     KRLSENDYDKVESMLNTLLRKRQGKIVHLADSSKLTPDISQKLSIEEREFFNQIYEIS
                     TKFSKKIVGDKK"
     gene            19768..21846
                     /locus_tag="QGF01_01040"
     CDS             19768..21846
                     /locus_tag="QGF01_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HJN19521.1"
                     /translation="MTAESQTKSAMSDQVKEFLTQFKDTTGSFSYVEQIDQMMSKRMT
                     HIMIDYNDLVSYPDIESKFNERPDDILKAFSKAIGEILHERFPQYSEKIKKVIRARIA
                     NYPVQRSLRQIKADVISKMTSVSGMVVRSSEVKPLAQELTFICPEEHITVVNLFQGIS
                     LDSPVQCSTPNCKHKNLGIVPESSRFIDFQIVRLQELPEDLPPGQLPHYIDVSIKQDL
                     VDNARPGDRIILTGIVRIEQERISGVSKNESALYRLRIDGNNVEFIGGRGVKSSRRSE
                     REEISAEEEKIIKSLAKNQDIYSRLIDSFAPHIKGHEIIKEAILLLIVGSNQRLLTDG
                     SKIRGDINIFLVGDPGTAKSEMLKFCSRIAPRGLYTSGRGSTAAGLTAAVVRDSTGIF
                     MLEAGAVVLGDQGLVCIDEFDKMRAEDRSALHEVMEQQSASIAKGGIVATLNARTSIL
                     AAANPMFGKYDQFKNIHENVNLPIPLLTRFDLIFIVRDIPSQVKDREIAKHIIDLHGQ
                     DGTDTKSLIDIDILTKYLAYAKRKEPTLTADAENTILEYYLKMRNVESEDMLTVTPRQ
                     LEGLVRLATARSRLLLKNQVDKEDAERAVFMMESMLHETGIDVNTGKVDLGVLQGRPR
                     SEISRIQTFMDILKDLEGEQKTPVEEQQFIDHLVKSEKFDEEGAKNYLRKMIRENTIY
                     ESKPGHYNRV"
     gene            21843..23999
                     /locus_tag="QGF01_01045"
     CDS             21843..23999
                     /locus_tag="QGF01_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJN19522.1"
                     /translation="MNISELNLPKKSIDFLLEQGYTELYPPQEDAVKAGIIDGKSVLV
                     SAPTASGKTLTATMAILSHLSKNKSKVIYLTPLRALASEKFEEFKKLEKINQDQKIKV
                     AISTGDFNSNSHDLDDADVIILTNETMDAMMTFQKSWIYEIGLMVSDEIHLIGDSGRG
                     PTLEMILTRFKTGYVGKKPQIIALSATISNSDKLAEWLDCKLVESEWRPVPLSEAVYS
                     NHTITNQDRVESEGNLDRNRESRHKKAEIGLGLDTIEDQAQSLIFTMTRASSVATATE
                     AGKFVVKQLKKDELEKLIKISKTILPKETEDQTKLVTKLADMVKNGVAFHHAGLDQRC
                     RTIIETEFKNGHIKLLTSTPTLAAGVNLPARRVIISNVFRFGSNGNAPITILEYKQMC
                     GRAGRPQYDDEGESIIIAKGSPHDYLEHYIDGEPESLESHILEDSSLRIHLLGLIATS
                     STFSSVSEKKINDFFSQTFGGLSDTALENKISERLKDLKEYDMITDEDGFKPTKFGQK
                     VFWLRIDPKTASNITSDLEHYVKGSKHTFGSLHMITNLPEFYPHFDVPEKLQEYMEII
                     YDNFKHEKLYAQQELQKNWTKSLLILYHWIDGMTYSDMSEKFDAEPGDIFQIRRNAEQ
                     LAYIIREIARFWKNQVLVDELDILRQRIRHGVPEEYLDLVRIKNVGRVRAKILYKYNF
                     RDRIDLKEVSVEKLAAIDKIGMTIAKSIKSQIEKVR"
     gene            24000..24989
                     /locus_tag="QGF01_01050"
     CDS             24000..24989
                     /locus_tag="QGF01_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-1-phosphate transferase"
                     /protein_id="HJN19523.1"
                     /translation="MQEQIIIAIIVSSIAFFSVYGLTPIFIKALASRNLTVKDVNKKG
                     DVMIPRPGGIPIVIGLVAAEIALYAFFPISEILAVIIVSILGFCVGFVDDRKVMGGWF
                     KPLALAFCASPILLLGAYDSNLSFPLFGLVQIPSLYLALVVFMIPITGNTINSIDVLN
                     GVASGVVTIASFALAISLFIMQNYEIGIASLCLAFASLGFYKYHKFPSKIFPGDSGAL
                     VLGSTYGVIAIVGHVEVISAIAILPAVINSFLFLTSVKKIVEHREIKNPTLRTEDFKI
                     KSTDDKTAPITLVRLLVAKLPLSEKQIGAEIFKLSIFCGILAVITAFFMEVKI"
     gene            25055..25213
                     /locus_tag="QGF01_01055"
     CDS             25055..25213
                     /locus_tag="QGF01_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19524.1"
                     /translation="MVLFIGPISFPENIDPKITSVVKISLALFLIFIWVLILTKIKNW
                     IFKSQLES"
     gene            complement(25223..25699)
                     /locus_tag="QGF01_01060"
     CDS             complement(25223..25699)
                     /locus_tag="QGF01_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HJN19525.1"
                     /translation="MISNYISDKAKIGKNVKIWHFAYVGDNVEIGNDVKIGSLAHVDY
                     NVKIGDNTLIEGLVYIPPLTRIGKNVFIGPSATLTNDPYPPSEKLIGITIEDNVIIGG
                     RAVIKAGVTIGKNSVVAMGAVVTNDVLPDTVVIGVPAKAKYSRQEYDKKQSEWKNA"
     gene            complement(25706..25975)
                     /locus_tag="QGF01_01065"
     CDS             complement(25706..25975)
                     /locus_tag="QGF01_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HJN19526.1"
                     /translation="MNREILEILACPIDKFHPLDLFECKTENEKIVEGVLYCKKCSRF
                     FPIIDEIPIMLPDDLRDKKRDLEFLQACIEQLPQKIVKEALPWHL"
     gene            complement(25972..26907)
                     /locus_tag="QGF01_01070"
     CDS             complement(25972..26907)
                     /locus_tag="QGF01_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HJN19527.1"
                     /translation="MKIIQIGTGGWGKNHCRVLSELGVLSAVCDKDLHLAEEFGKKYS
                     VNFYNSLETLLEKEEFDAAFICTPTITHADIAAKLIESKKHVFVEKPLTYLSEDGQKL
                     VDMANKNNVVLTCGYIERFNPAVSTVKDFLTSKKYGDLIRLEFYREHRMPQHIKDVGI
                     IYDTAVHDIDTAMWLFGKTPEVVFAIAGKINHEHEDFATIMLGFKDNKTATISSNWIT
                     PTRVRNFNAVCTDGRIFSDFITQEIRIETDHGTENPKNEKTEPLLLEIKNFLGAIETK
                     NDIKVKAEQAVNVTKIAEAALLSSQKGIPIYLDIK"
     gene            complement(26908..27825)
                     /locus_tag="QGF01_01075"
     CDS             complement(26908..27825)
                     /locus_tag="QGF01_01075"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HJN19528.1"
                     /translation="MKFLFSKYVWFDGKFVLTNKAKVPVTTHAIHYGTSIFEGIRAYW
                     NSEKLHIFRLKEHVKRFRNSGKFYDITLNFSDKQIENAIINLCKKNKIKKSCYIRPFY
                     FVGDYGINLHVTKKAPTHVSIFSFHFGDLFNKNGITATISKWRKFSDMSTPTQAKMGG
                     NYLNSILATQDAKQKGFDEAILLDQHGNVSEAPGENIFLVKNNQVITPPLSSSALDGI
                     TRKSIITILKDMNIKIVIRKIRKNELNLADEVFLSGTAAEITPVIKINRRKIGTGKVG
                     NITKLLMTTYSNIVMNKNKKYSKWLTRVY"
     gene            complement(27855..28457)
                     /locus_tag="QGF01_01080"
     CDS             complement(27855..28457)
                     /locus_tag="QGF01_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19529.1"
                     /translation="MSIAIYLAHLNPVTNAHVEIIEELKQENKVVVMPVRFLKEEQEI
                     NTKSFPFNFEIRKKMIESVFGDTVSVSSNYSFHAPFKKYFPPLISPKSWALKKQILQG
                     IESDYFTYTGDKAEGLMLKLYRLNPKVGIRKPISAKDVKNQIYAAVQNNDMEWKKSVP
                     ENIAQIISENWDIITKFASGEDMTTRVAGMKFPKEGYNSK"
     gene            complement(28614..29459)
                     /locus_tag="QGF01_01085"
     CDS             complement(28614..29459)
                     /locus_tag="QGF01_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJN19530.1"
                     /translation="MAIDKKAIITYVQLLKEDLAVFRLVPTDGVIPNYEAGQFITVGM
                     PIPSEDNKIIRRAYSIASHPENKKFIELVVRWVRRPLPGRVTTALFNAGEGDEVSWIP
                     PTGAALKINEKMGDGSKDQRRIVCIGGGTGIAPFMSFARHLHDLGDKRELVCLHGSSY
                     VDELSYKEELTALDQESLDRGSNKWNFKYRAAISRPQEWFNRSWTGQTGRVEQFLKPT
                     SSGKSPLEELVGEKVTPDNTIFYVCGWQGTVDGVLDYVRPKGFLLEKEKDKKGNFSVK
                     FESYG"
     gene            29571..29644
                     /locus_tag="QGF01_01090"
     tRNA            29571..29644
                     /locus_tag="QGF01_01090"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29605..29607,aa:Ala,seq:ggc)
     gene            complement(29626..30159)
                     /locus_tag="QGF01_01095"
     CDS             complement(29626..30159)
                     /locus_tag="QGF01_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HJN19531.1"
                     /translation="MDESKIDKTEISEHIHTQNPEIIKKIISKLDDQNIQVRGEAFYS
                     LFLNENNISDILIDGLNSESNNVKGFLSLVLANRGDSNAIPHVERLIQDTNSMVRSYA
                     LSALAHLNARQSINLIRNCFDDKKIEVRKNAVQAFFKIGGKIFDNEIEKLSENADEEL
                     IFLLEKYEKMSNGPEGI"
     gene            30381..30632
                     /locus_tag="QGF01_01100"
     CDS             30381..30632
                     /locus_tag="QGF01_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HJN19532.1"
                     /translation="MGKKPLMAYVTSTLIQLANVKSVSIVARGMSISRAVDVAQIISR
                     KTEHTGYSIGDIKIDSVPMESKDGRTRNVSTIEIKVERK"
     gene            complement(30649..31506)
                     /locus_tag="QGF01_01105"
     CDS             complement(30649..31506)
                     /locus_tag="QGF01_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HJN19533.1"
                     /translation="MKSVRKLLQDKSKPLVMPGVYDAIGAKIVEKVGFEAMFQTGYGT
                     SATLFGMPDYGFIGSTETVDNARRICHAVSTPVIVDADTGYGNALSIWKLVQELEEVG
                     ASGIFLEDQRWPKRCGHMQGKEVIQKDEYAEKLQAALDARSNKDFIIVARTDARAIEG
                     LDKAIERGLYYKKIGADAIFIEAPKTIQEMKIIGKSINAPLVANMIEGGATPVISKSK
                     LHKMGFKIVLYPLSVLFSNTYSTLKLLQELKKSGTTRKLSKQIVNFNQFNDIVELSKY
                     RKLEQKYKK"
     gene            complement(31537..32043)
                     /locus_tag="QGF01_01110"
     CDS             complement(31537..32043)
                     /locus_tag="QGF01_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HJN19534.1"
                     /translation="MIGDWIQRVGSSIPRGFSRFFILELLKKKSYTGKQIIDSAIKQS
                     GGKWKPSPGLIYPLLGRLLDDGLISEDKDGKYRITKKGRKTTDDLQTINEIVKNQLDV
                     LFRLGSVGRFVILDMLERLSVIGSLLSENAAQMTKEEIKKYEKFLKSELDKMEKMSNE
                     REQKIKID"
     gene            32157..33869
                     /locus_tag="QGF01_01115"
     CDS             32157..33869
                     /locus_tag="QGF01_01115"
                     /EC_number="1.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HJN19535.1"
                     /translation="MKNSLEYDLIIVGSGLAGLRATIQAARRNSKIRIGVISKVQVMR
                     SHSVSAEGGTAAVLFPDEGDSIESHIYDTVKGSDFLADQDAAERLCKEIPNEIYQLEH
                     WGMPWSRREDGKIGQRAFGGYSFPRASYASDKVGFFEMQTLYDTCQKFDNIEFLNEWF
                     VTSIVQSGKKFAGITAIELSSGNFYTIKGKALIIATGGAGRIYSFSTYALSSTPDGLD
                     MAYRAGLGLKDMEFVQFHPTGIMPSGILITEGARGEGGHLLNKDGERFMAKYASKLME
                     LAPRDIVSRAIMTEINEGRGFKNETGVDCMKLDLRHIGDEVIKQKLGGIREISIKFSG
                     VDPAKELLDIRPVCHYMMGGIHTDINGASELKGVWAAGEAACISIHGSNRLGANSTSE
                     CIVWGKITADEAVTYIEKESSSESFPDHLVYAEERRIYDGIFRGKGEVNPYEIKQKLT
                     DTLTEKAYVFRNETDLVEGLKKVRELKAMTWKHVDDKAKEYNTNFSNVMELDSMFRIA
                     EVVLIGAINRKESRGSHARTDFPKRDDKNFLHHTLAYYDPQEPIMKTHPVIITNYKPV
                     ERKY"
     gene            33869..34297
                     /locus_tag="QGF01_01120"
     CDS             33869..34297
                     /locus_tag="QGF01_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HJN19536.1"
                     /translation="MEKDRKEGIKGMANPGRYGIERVAYWLMRITGLGLLFYFIGHIY
                     ETSNLLDGKAAWNSMLELTQTTEGHIFLTLVIGMCVFHTGNGIRLMIGQAGFGLGKPS
                     RADYPYTPKSLNMKGKLCIYVSIGLAALAMMYGLGVMYDV"
     gene            34302..34652
                     /locus_tag="QGF01_01125"
     CDS             34302..34652
                     /locus_tag="QGF01_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HJN19537.1"
                     /translation="MRESHIMKIHYLTALIAVGFVIIHIMVRVTQGFANSLEFESVIA
                     NYKSIPYAIVLEAMLILISVHGFNGLRIILLELKQGNAYENAVTYGCLVAMIALIAYG
                     SRTIIMASMGMVEA"
     gene            34649..35449
                     /locus_tag="QGF01_01130"
     CDS             34649..35449
                     /locus_tag="QGF01_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HJN19538.1"
                     /translation="MTKDGALPKGFERPSSLPSQEKYEKVKSSSKTTTLRISKFNPES
                     DSTSTFSEFKVHYQRWTTVLDAILEVKSNYDHSIGVRYSCRQATCGSCGMIINGKPRL
                     ACFTKISELDSDLVTVEPMNNFPIIRDLAVGFERLIATHKRIKPYIIRDDSEVTSETK
                     EFLQSPEDVEKYIQFAGCIKCGLCNSACPTMATDSSFVGPQALAQAYRYVADNRDKGK
                     DERLKIIDEPHGIWRCHFAGSCSQVCPKGVDPAMGIQLLRGYLLGYRK"
     gene            complement(35450..35812)
                     /locus_tag="QGF01_01135"
     CDS             complement(35450..35812)
                     /locus_tag="QGF01_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HJN19539.1"
                     /translation="MSQELKQIRVKIFDKLSGIVDPEINTSITELELIDEVDIQDTNV
                     KVDLHLTSPFCPAVFGFKICQDIHDDLLSIDGIDDVKVNVSNHFMAEQINNQVNNSPN
                     PHKKETKTEDESSKSSEE"
     gene            35863..37323
                     /gene="argH"
                     /locus_tag="QGF01_01140"
     CDS             35863..37323
                     /gene="argH"
                     /locus_tag="QGF01_01140"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HJN19540.1"
                     /translation="MYRSRLGKNLGKITLDYVSSLNDDIQIVMYDILGSQAHVIMLYE
                     NKIIVKKDLKKILTGLEFLKKENFRKKSSSEDIHELIESLLIKKIGIDAGGKMHTARS
                     RNDQVALDIRMKIRDDTNIICQCLLDTIKSLVKLAKKHQETIVPLYTHLQQAQVGLFS
                     HYLLSYADALFRDLDRFYTTYDRINENPLGAGPIGGTSIMIDRQSTGKMLGFNGIVEN
                     SIDATTTRDFAIEYIAASAILMTNLSRISKDFSIWSTSEFSFLELSDEFTSPSSVMPQ
                     KKNPDILELTRGKTSQIIGNLTSILSTIQVLPSGYSRDLQQVNPSLWDTSHTVISALL
                     IMESMLNTIIINKKNMQKAAEDGYLIALDLAEKLVHHDISFRKSHQIIGILVKAAHKS
                     RKSISALTKLEVKNVMKGEKVNLELLMKLIQSTTVMSSLNDRKSQGSSGISEQKRMVI
                     HRMKKIDIYQKEVKKRSNNIQNSLDSLNKKVKTLIK"
     gene            complement(37326..37399)
                     /locus_tag="QGF01_01145"
     tRNA            complement(37326..37399)
                     /locus_tag="QGF01_01145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37363..37365),aa:Lys,seq:ttt)
     gene            37490..37567
                     /locus_tag="QGF01_01150"
     tRNA            37490..37567
                     /locus_tag="QGF01_01150"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37526..37528,aa:Asp,seq:gtc)
     gene            complement(37568..38368)
                     /locus_tag="QGF01_01155"
     CDS             complement(37568..38368)
                     /locus_tag="QGF01_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HJN19541.1"
                     /translation="MDIIEILRSASKLVYENVKDLPGTEEAVAGDFGRGAGGDISRNI
                     DIIAEKTVVDYFKEINFECTVLGEECGKVELSSNPKGFVIMDAIDGSANAVRGVPFFC
                     CSLAFATDDKLSSITDAAITNLSTGDIYWTSKEKGAFLNDKPISVHKEKPLYKIVGIN
                     SSGTSPELLKRLSPIYEKHHHVRHFGANALEMALFAQGLIDIFIDLRGKIRVQDIAAG
                     YLLIKEAGGLILDENLEPLDSDLNYETRLSFVAATNKEILSEVFSLIK"
     gene            38445..39563
                     /locus_tag="QGF01_01160"
     CDS             38445..39563
                     /locus_tag="QGF01_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19542.1"
                     /translation="MSGKDESVTSKSSLKGTKAGKKIIKQYFFKSKGYRQFNKYKKEY
                     ETNFPEFAKRFTNDLLQQIKDDSSPNITQQKFGEEIGSTDIILGSSEIDPIKSKLESF
                     EILNDRVLRILNSNFVKMTFPVFYGLFDASAEYFHDSNDPKLREDIIDGHIIAIDLSE
                     PMDRIMDRDEDLDYLDDYKLMNPYILKLARDKIAKGGEEVLKQFESGFRDARIGQYID
                     ATLKQNPTSMTEEKLDESYKKYRSVMGTAGCNMALSRQPLGEIFQIGMGKASESVGCG
                     NEIEDSIRDKAVKIPSWPLYYSLLENDVRKGFDLTMKKSESYLREARNALESLPKNFS
                     HREFLEFLFLTVEHYNEFWFNRLQHENIWSDLQSNLPK"
     gene            39572..40729
                     /locus_tag="QGF01_01165"
     CDS             39572..40729
                     /locus_tag="QGF01_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN19543.1"
                     /translation="MWSEKYRPKNIIDLIGNEEVRSDFVDWFTKWKKGTKPILLVGPP
                     GIGKTTLAKLAAKQFGYDLIELNASDVRSKGRIQNILQPILGSESLLGHPMIFIDEVD
                     GIHGRADYGGAETLIKILKEPTVPILLAANSDISTKMKSIKKAVRTIHLKPLSPRMMQ
                     LYTNIILKKEGVSLTPKSLQKIVIESRGDLRSMINSIQANVTGFEPPTEKSFERLDIE
                     EGINTFFKSHSIDEARMILYSMHIDPREKINAFYSSIITSNIDKKNLAYMLEIISKAD
                     MLYGKIMKTQNWRLLRYLDSILLGLYKPNTSIRYSRFNLSWPMINRIRWDGKKIKLLS
                     KIISKNLHISVSTFTTFVFNTMLFCMKNDKLDFELDEEFDDIIEKEMSLIK"
     gene            40726..41049
                     /locus_tag="QGF01_01170"
     CDS             40726..41049
                     /locus_tag="QGF01_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19544.1"
                     /translation="MSWIKITMKYDGTCIVCNKKIRAKEIGFWLKEESKVKHEKCAEV
                     NKELKCAICNGSAGCMDCEFAEICDRENVSKLCICKKCESVHDSFTLYQKSIIEKFPL
                     LNLKI"
     gene            41069..41800
                     /locus_tag="QGF01_01175"
     CDS             41069..41800
                     /locus_tag="QGF01_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19545.1"
                     /translation="MSEGKKEETQKETGPVTLSEAEAEAAVAKAEYEAAKEEAEDAKP
                     DDGTLRSTTISDAVAASELAATAETAYEAAKEKVKAARIASEAAWKVEYEAAKANAGE
                     ITKDEFTHKRKQDRIFRREMGEPEFFGNAKNIPLKAMHTQQEEEEISRKVQIPTDQTP
                     KYNPENLLSPEFAGISNYESGLGDILDESKLKLERLLNDPNSTVQEIELAKTNLKKLN
                     YLFENYHLGMNVFRTAKGGRDKIKE"
     gene            41812..42864
                     /locus_tag="QGF01_01180"
     CDS             41812..42864
                     /locus_tag="QGF01_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HJN19546.1"
                     /translation="MAKNKFNILNARITFKNVPIHKITNYSFKDISTASESFKKINGV
                     SECIIIQTASRVEIFLVSKSEYFAVEKIKEVWTSLTELEQIDIDHWDQTLEVYMNTDV
                     YRNLLRLASGIESVVVGKEEILDEIKNMSSSAKQAGNSGKVLDKLFDSSIRIATDIRN
                     STGIGIGIKSIGDIAVNLAEEKVGNIGSKKILLIGTGETAAMVAKCFNKQNHGFDVAS
                     MSIERATGFSKILGGNPVNFEDVIGFPKYDIVFVATTADYFLVSAKDMKKSMKKRKSG
                     IMILDLSDPRAVELQVGMIPKIKALFRDEISELDDESGTRRKKTSTVEEAISKEVPIL
                     EESMKQLKQGNIITAN"
     gene            42932..44479
                     /locus_tag="QGF01_01185"
     CDS             42932..44479
                     /locus_tag="QGF01_01185"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HJN19547.1"
                     /translation="MHSDKINEFLKKRKGSSHAGGQDKISKQHEKGKLTARERVNLLL
                     DEGSFVEIDALTTHHYYEYDMQKKKFHGDGVIGGYGSVNGRQTYVFAYDFTVLGGTLS
                     QMGAKKITKLMDHAIRNGCPIIGIMDSGGARIQEGILSLDGFGDIFYHNQLASGVIPQ
                     ITASIGPSAGGAVYSPAMTDFVVMVNKLGTMFVTGPDVVKTVLGEEVSFDELGGAMTH
                     GSKSGVAHFVVQNEYECMDHIKTLLSYIPQNNIEEPPIVVNGDDPNRLDHNIINFVPE
                     DPVKPYDIKKIIYSIVDDHNFFEIHELFAQNIVVGFARMHGRTIGVIANQPLFLAGAL
                     DIDSSNKAARFIRFCDCYNIPIVTLVDTPGYMPGTNQEHNGIIRHGSKLLYAYSEATV
                     PKITIVIGKAYGGAYIAMGSKNLRTDINYAWPTAQIAVLGSEGAVKIMNRKELANSKD
                     PESLKKQLVDEFTEKFANPYVAASHGTVDTVIDPAETRPMIIKALEMLSNKRDKQLPK
                     KHGNINL"
     gene            44485..45972
                     /locus_tag="QGF01_01190"
     CDS             44485..45972
                     /locus_tag="QGF01_01190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HJN19548.1"
                     /translation="MISKVLIANRGEIALRVIKTCKALGIKTVAVYSDEDVHSLHVKN
                     ATEAYHIGEAAPAKSYLNQEKILEVMLSSGADAVHPGYGFLSENDDFARLCEKNKINF
                     IGPSADSMNLCGDKMRCKEAMLKAKVPTVPGSPGLVKDADEAEKIANEIGYPVMLKSV
                     YGGGGRGIRIVNTDQELQDGYETVTAESIAAVGKSAIIVEKFLEKTRHIEYQMCRDHH
                     GNTVHLFERECSIQRRNQKLIEQTPSPVVDEAKREEIGELVVKAAEAVDYTNLGTAEF
                     LRADSGEFYFIEINARLQVEHPISEMVSGLDFVKLQLDIANGEPLPFKQSDLKMNGYA
                     IECRINAEDTFLDFAPSTGPVPDVTIPSGPNVRCDTYLYPGCTVSPFYDSLMAKLVTW
                     GRTFDESRLRMLNALNDFYIQGVETSIPLYKTILNTEEYKNGKLTTDFLKRYNIIERL
                     EEDIKNQRNDKQIEAVASAVMYSEFFQSKIKSSNTTNSLWKSLMD"
     gene            45979..>46220
                     /locus_tag="QGF01_01195"
     CDS             45979..>46220
                     /locus_tag="QGF01_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HJN19549.1"
                     /translation="MEFKLEDVEETFNGEIINKISNNEYLIKIQNKEHQLQILNINSN
                     GMEFVLDNKFHNVKYVENNTAEMRIVIDGVSLIINM"
BASE COUNT        15679 a         7345 c         8169 g        15027 t
ORIGIN      
        1 ttgaagctgc aaataagatt ggatattctg tagttatgaa gattgcctca ccacaaatag
       61 tacacaaatc agatgcaggt ggtgttaaag tcaatcttac aaatgatgat gaagttcgta
      121 atgcattcaa agaaattgtt gagaatgcca aaaaatatga tagtgatgct gaaatcaagg
      181 gagtcttaat tgtagagatg gttaaaggtg gtaaagaaat gatcataggt tccaagcttg
      241 agcctggttt tggtccggtt attatgctgg gtatgggtgg aatttatgtt gaaattttaa
      301 aagatgttac tttcagactt gcaccattaa ctgatcaaga gtcaaacgat atgattatgt
      361 ccattaagac aaagaagctc ttggatggag taagaggtga aaaaccatct gatgtagaga
      421 aattatctga gtgtattcaa cgattatccc aacttgtcac tgactttagt gaaattaaag
      481 aacttgatat gaatcccgtg ttggtcatgg aaaaaaataa tggctgtaaa atattggatg
      541 taagaattgg tatctaaatt agctaaagct atggctaatc tatttctaga atttttataa
      601 gagaatatta tagtagtaac atgcaaaaaa caatcagacc cataagaact ggcgaagaat
      661 atattgaaag cctgagaggt cgtgatctta caatttactt gtttggtgaa cttgtcaaag
      721 aacctgttga tcacccaatg attcgtcctt caattaatgc agttgctaaa acttatgatc
      781 ttgctgtgga agaagaagat cttgcatcag taaaatcttc tatcacagga gaacaggtaa
      841 atagattctt gcatattgca gaaagtgcac aagatgtagt taaacaaaac aagatgcaga
      901 gaaagttagg acagctgact ggtacatgtt tccaacgttg tgttggtatg gacgcattaa
      961 actcacttca ttctactaca tttgagattg acgaaaaaca tggaacggac tatcataaaa
     1021 aactgttaga gtttatcaaa atggttcaac atgaaaatct ggtgattgga ggtgcaatga
     1081 ctgatgtcaa gggtgacaga agtatgggac catctgacca agaagatcct gatctatttc
     1141 ttcgtatagt tgaaagaaac gataaaggtg tttacgtatc tggagccaag gctcatcaga
     1201 ctggttgtat taactctcat tggatgcttg ttatgccaac tatgagatta actgaaaacg
     1261 ataaagatta tgcagttgta ggagcaattc cagtagatgc accaggaata acttacatct
     1321 atggtagaca atcttgcgat acaagaagta tggaacctgg tgatattgat caaggtaact
     1381 cagaatatgg tggtcaagag gcgatggtaa tttttgataa agctttcatt ccaaacgaaa
     1441 tgatcttcat ggatggagag tatgaatttg cttcaatgtt ggttgagagg tttacttgtt
     1501 atcatagaag aagctatgtt tgtaagactg gtttaggtga tgtactgatt ggtgctgcag
     1561 ccactatagc agattataat ggtgtgccta aagtttccca tattaaagat aaattaattg
     1621 agatgactca tctaaacgaa tcaatctatg gtacaggaat tgcatcttcg tatcaatctc
     1681 aaaagatgaa gtctggcgtt tggcaaaatg acgatatgct agcaaacgta tgcaaacaca
     1741 acgttacacg ctttccttat caaataagca gatttgctca agatattgct ggtggattaa
     1801 tggttacatt accatcggaa gcagagctca gaaacccaat aactggacca ttacttgaaa
     1861 aatatctcaa aggaagaaaa ggtgttgatg ttgaaagtag gatgagaatt ttacgtctaa
     1921 ttgaaaatat gactatgggc agaaacgcag ttggttatct tacagaatca atgcatggag
     1981 caggttctcc acaagcacaa agaattcaga ttgctcgcca gatgcaactt ggttataaaa
     2041 aacgacttgc aaaaaatcta gcaaaaattc aagatgatgg tgaggaaact cttgagaatg
     2101 cagattattt taaacgcgtt tttaaactag ataattccaa agaataagat cattctttct
     2161 cttcttctga tttctgttcc ttatcacgga aaaattctgg tatatcatct attgatttag
     2221 tatcttcttt actaattcca tcggtagatt ttccatcatt agctagaaaa tcattagaac
     2281 tatactcttt atcatttaag aaataatcta ttttttcgtc tgatttggat tcatcggtat
     2341 catttattat cagatccttt ttgttgtatt tttttgctat ttctctaatt gttattattc
     2401 caataattac agctattatg atgtaattta ttggaggctg taaaattttt gttatataac
     2461 caacttgtgg aataacatga accacggttc caacatactc ttgctcagtt attgggaagt
     2521 ctgtaccagg aatagatgcc tgattattgt caccttttgt tctcaatgtt tttgggtcat
     2581 catcaactac tgctaccact ctatgtacga tgactttgtc atggtctttt ggtctatcaa
     2641 atacaataat atctccaatt ttgacgtctt caaatggcgt atgtccagtg attactataa
     2701 tatcatacat tgctaattgt ggatacatac ttccactaga tacaacataa aatggattta
     2761 cggcaccaaa ataagcctgt aaaccaatcc atattactgc cacacaaata gccacaatta
     2821 tgatatcctt tactatactt ttgattgaag atgatcccaa gttacgtttt ttccttcatg
     2881 atgattagat aaatcttatt gtgcttacta tactttcgat ccacaatttt cacaaaactt
     2941 agcatctgat ttgttatcaa aattacagtt tgaacagctc aatccgtccg gagctggaat
     3001 attttctctc tcagttacag tatttccgag taatacaatc tcaccaattt ttttaatttt
     3061 ttgccaatca acacttttgt catttccata attatctgtc ataactaaaa ctacttcatt
     3121 tttggaattg acacccactt gtttagcaac cccaatctgc ttagcatttt catcaaatac
     3181 tatttttccg gtgattgcac taaatgtcga atctatatca cctgattcaa catcagtttc
     3241 taaagacttg tctacatttt cgaattgctc attattttca ctaataattt cattcattat
     3301 actttctgaa tcttcttgag tatcattgtt taattcctgg aaaatttctg cattcacttc
     3361 agttggcttt gcattaccga tgtcacttga ttcatctgat ttttgaaatt ctctatactc
     3421 agcaataggt gaatcacatt caatgcaaag atattgacct cgttcaacaa gaatcaaatt
     3481 atcagctact tgctcgttag gatgttcata ttcaattttt ggactgccgt catattcttt
     3541 gtcacatata ttgcaagaat attttttaat tgtatattca tccagtctac ctataggtgc
     3601 caaaaagaga tctggaccac caaggtttcc ttttctttgc tgttcatcgg aaacttgagc
     3661 catcacatat cctcctgaac ctcttaactt ttttattcgt aaatctgagc tcataggtgt
     3721 tcatatctaa aatctcattt aaatatattc tatgaactca taattcagct gaacatttaa
     3781 gtggcgattt tttttagggt caatatggct gtggttcacg tttcagatac gaaaacttgg
     3841 gaaacagatg ttataggttc atcaacacct gtttttgttg atttttgggc tgaatggtgt
     3901 ggtccatgta gaatggttgg acctgttgta gaagaactgt ctggtgatta tgatggcaca
     3961 gttaaatttg tcaaagtgaa tgttgatgag gctaatgaat tggcgtcaaa atacaatgtt
     4021 ttcagcatac ccactttaat gatattcaat aatggtcaag tcgttgctca acaagtaggt
     4081 gctgcttcta aggaaaccta caaaacaatg attgataagg cactggaata atttagcctc
     4141 aattattttt tgatagaacc taaagtaatt ataattgtaa aatgtgatta aaatttatgt
     4201 cactaggaga agttgataca ttaaaccttt tgactgataa attaaacaac ttattcgaag
     4261 aatctcaagg ttactacgaa tcatttcttg acactaacaa tatgtacaag gaaggaaaat
     4321 tgacggaaag ggaatttttt cagaaactgg gtgattatgt tgttgcatat tccgcattgg
     4381 aatttttatc aatcaaagtg atctttgaaa ttaaaaaagc cgttgataaa atttctggcg
     4441 gtacatcatt gggtggaaca caatctccag gtttgatgcc aggaatgggt acgccaggaa
     4501 tgatgccagg tggtatgaca actccaccag gaccacgttc tggaactgca gacaatcctg
     4561 ttggtggtcc agcatctgta atatctgctg caaccgcttt cgataatgca gggacactac
     4621 caacacctga tccatcactg gtttcaaaac catcaaacgc tgcaaagtgt tcctcatgtg
     4681 gttctgattt gcgaccaaat gcaaagttct gcacaaactg cggaaataaa atttaatctt
     4741 taatttttac tattcttgac ctgcaccaca ttcagtacaa aatcttgctg aggcagaaat
     4801 tttttcacca cattctgaac aaaattttgt ttgactacca gaatcagtat ttgtactatc
     4861 ataaagtcca gattttaaca cagcgccaga tggttcataa ctatcatcgt ctataacttg
     4921 agttggcgca aagtcttttt cttctatgta ggtcattaat ctaggcattg tttcttcttt
     4981 attatttgga tcagggacaa tatctccaag ccaaaatgaa aatcgtttga gggtcaaatc
     5041 aggagtagaa aatgttaagg tatgagctgc catataatct tctgcagcct gttttccatt
     5101 gaaaactttc attacacatt tttctatgat ttatctataa tagtttatag atggactggg
     5161 agggaattga accctcgaca tccgcgttgc gaacgcggca ttataccact aaaccaccag
     5221 ccctgtaata ttcatcataa aatcagtttt tattcatttg ctcaagtaaa aatactagaa
     5281 ttttgcaata gaattagatg acgtatgata cattgatcgt aggctcgcat attatcacac
     5341 ctactggaat aattgataaa aatatgattt tggatgaagg aaaaattgta aagttaacaa
     5401 atgaaacacc atcatgcgat catagaatta atgctgaggg attagtatcc attcctggtg
     5461 ttatagaccc gcacgtgcac tatggagttt actctccaat agaacaggct gcacaatctg
     5521 aatcacatgc tgctgcaatc ggtggtgtaa ccaccatgat gagaatgctc cgattaggtg
     5581 actcttatga aaaatcatta tcagatcaac tgacatccag ttcaaattct cattatgtgg
     5641 actatgctat tcatgcatca atttttactt taaaccaagt aaatgaaatg cggttttgtg
     5701 tagaaagtgg agttacctct ttcaaactgt acatgaatct aggaggagaa gttggacatg
     5761 tgtatatgga tatgaaacct ggggagaatg tcttacgaga agaatgtgta gaagtaaact
     5821 ctgaaataat tactaaagtt gtacaaaatg cagcctcact tggatgtcct gttttagttc
     5881 acgcagagga ttacgaacaa tgcggatgcg gaataaaaaa gtctaaagaa aaaaatcttg
     5941 acggactttc tgcatggtct aaaagcagac cttctgaatc agaatcacat tcaatcaaaa
     6001 caatttcaaa acttgctaga gatttcaatt gtgttttata ttttgcacac ataggctctc
     6061 gtactgcctt gcaacaaatt aatgaagaaa gaaaaaatgg aacaaaaata tttactgaaa
     6121 catgtcctca ttatttgaca ctatcttatg aaaaacaaga aggatatctt gccaaagtaa
     6181 tgccacccat aagaagtgat gatgatgttt tagctgtatg gaatgcaatt aatgaaaatc
     6241 aagttgatac gattggaaca gatcacgttg caaatcaatt aaagctgaaa ctagatggag
     6301 atactatttg ggatgcatta gcagggtttc caggtattgg aacatcttta ccattgttac
     6361 tcagtgaggg tgtaaacaaa aatagaatta cattacaaca gttggtaaat attactagta
     6421 cgaacaccgc caaaatattt ggaatgcatc ctacaaaggg ttcatttgag cctgattcag
     6481 atgcagatat caccttaatt gatctaaata aagagcaaaa agttacaaat gatctatttg
     6541 gtggattttc tgattatcta atctatgaag attggaaatt gaagggttgg ccagtaaaaa
     6601 ctattgttcg tggacaaaca gtgtctgaag atttccaagt tgtcggtaaa tgtggttatg
     6661 gtaaattagt gcctagaaaa aataattcta tttgatcaca tcaaagcgtc catcttttaa
     6721 tgccttgtaa atgatgtctg gttttctggt gaaaatacct acctctaaaa ctccaggtat
     6781 gctttgaatt ttttcctgca atgcttttgg tttttttatt gttttaaaat cacaatcaag
     6841 aattatattt ccattttctg taacaaatgg atatccccta tcaagtgttc gaatgcttgg
     6901 ttttccacca atttttaaaa tatcttttgt tgctgtattt ctagctaaag gatgaacttc
     6961 aactggtaca tctctattga aatttgttac aaattttgaa tcatcagcca taataactac
     7021 tttctttgcg gcactaatga gaatattttc tcttagcaat gcaccaccgc caccctttat
     7081 caaatttttt tgtttatcaa tttgatcagc tccatcaaaa acaacatcga tttgatcttg
     7141 gacagtatcc ataagatgaa gttttccctt ttctgcttcc aatttaattt gtaaagaagt
     7201 tggtatacat tttacatgaa ttttattttt ttttacatat gaggatagtg attttacaaa
     7261 tgcagttgcg gctcttccac tacctaatcc aatcacttgg ttatttttta caaattttag
     7321 tgcatcctga gataatgcct taatggcact atcatatgtc aattattcta cctctacttg
     7381 atctagatta gataatgatt tttctataga tgctactact ttagtaatat catcttcatc
     7441 aagtgattca tcaacatctt ttacctgttc ctttgtgttt ccgtttgtga tagggggttt
     7501 aatctcagtc tgttgagatt ctactgcttg ggaacttgga ataattactt cagtttcatt
     7561 attttcaaat tttgtttgat ttgaattact atgtaacgta caatgtctat ttgtgaattt
     7621 tggatttgta cactctggac gttcacatac attagtttca ggtactgcaa ttttaaccac
     7681 attttctaaa ttttttacta ctggaatatc agtactatgt tgtatggttt ttgtttctaa
     7741 ttgtttttct ttaaatttag tcagaaaaaa ttctggtatc tttttaggta cttcagtcat
     7801 agtaataacc tcaagatctt tttgtgattt tgagtctatt ttcatatttg atgtatagag
     7861 atttagatct tcattctttt ttgctaaaat ttgagaatca accttcttag gtttttgagt
     7921 tggtttttgg ttggccttat acacatcttc attaggtctg gtgaatttta ttgttaattc
     7981 ttgtaatttt ttttctattc ttgataactt ttgatccatt tctttgtagg attgatcttt
     8041 tgatgataaa acatctggct cctttttact ttctaaattt ttaatttttt catttactac
     8101 cctaagttga tattgatatt ttgtcaaaag tttgtcttgc tgtattcttg ttaattgagg
     8161 gtcttgttga tataatcttg aaatagattt tgtaagaata tctcgttcta tgaccaatga
     8221 attagattcg ttttttatac tatagttaat ttcttgcatc taggtgtacc atttgatcca
     8281 agagtatttt cttattttgg aatctgggaa tccacaactg gcacatgtgt gatgacgttt
     8341 atgatatgaa tttttaccac aacgtctgca tctaatgtgc acatgtttcc tagtaaaacc
     8401 acccatggat gttgtacctt ttaccatagc attttcacct ttaggatggt ggtggagata
     8461 tcattatgac attatctcct ctcacaacta tggttccaag ttctttagaa tcgccttcag
     8521 aagggatctc ttctgattgg tcgagtagca ggttcatgtg ttggtcaaaa cctagaaggt
     8581 tacctcttat ggtttttgag cctttgagct tgatcagtac tgttttgtta atactctcat
     8641 ccaatatttt tactgccata tcaacggaca tattttacac ttattgtgac tgattacgaa
     8701 gccgtatatt aaacattcac tggtataatt tccaaccttg ttcagtaagt caagattgac
     8761 actcttttgc cagatttcta acaatttcag taaggaattt ctgtccgtcc ttctttgttg
     8821 caccggttgg gtcaccccac actccattct ttgtaacctg aggaaatcct cctggttgtg
     8881 tagaaagttt gctaattctt tgtatttcct tctcgctcaa gccctttgtt attaacccct
     8941 ttttcgcctt tttcatcttg accttgtttg aaatagcaag cattattgaa gtctctacaa
     9001 atccagcatg gtcaaattca tggttcatga aatgccaata tgagtaaaat cttattttcc
     9061 gcattctagc aggtctttgg agtattgtct ttctttctgc tagctttaat gatagagcat
     9121 ttccatgatg accattcaaa attataactg atttgagttt gttttttact actgatctat
     9181 caatatcgcc tatgatattc caaagagtat tactttccag actcacattg aaaaagggat
     9241 cgtgttcctt agaaacacca taactaattg ttggaaaaac tatgaagcca catttttttg
     9301 ctaaacgtga agcgacctcc gtcacaatat cagaatcagt tgttattggt aaatgagcgc
     9361 catgttgttc aatagagcca acaggaatta tgactgtagg tttttttgac ttgattaatt
     9421 ttctaaattc tggatcatat agatcgctag cgtccattac tttcacttag atttaacgtg
     9481 gacttttctc cagcgaactt ctaacttatt atctagatgc atttttacag ccttaaagag
     9541 agtagtagcc tctaactttt ggccattcct ttttattgat tcaagagtat cttctggagt
     9601 tactttgaag gaatcctgga aaattattgg tccttgatct agattttctg taacatagtg
     9661 tgatgttaca ccaacaattt ttgttcctcg ttcaaatgct tgtgcataag caagagatcc
     9721 aggaaatgcc gggagcagtg atggatgaat gtttataatt ctatccggat atctccaaac
     9781 aaagtttgga gttaaaattc tcatatatct tgcaagaact attacatcca cttcaaattt
     9841 tttacaaagt ttgagtaatt tttcttcagc cttttcttgg tttctatcat taacaatgat
     9901 aaatggaatt tttgcttttt ttgcaagtgg tgcaagagct ttttccgtac caacaattac
     9961 agaaatctta cctttgacag tacgtcttgc tgataaaatc tcttttagac aatgatcctc
    10021 ttttgttaca aatatagcga tatttttttc cgagttagtt tcagagtgtg tactaacctc
    10081 cattcttatc tttttaccta gtttttgtag ttccgaatct aatttcttaa aattaatctt
    10141 agaagtaaag gatacttcta gatacatacc aaataagccc tttattacat tctgatttac
    10201 tttttcaata ttaccgtgct tttgaaatac gaaatttgtg aattgtgcta cgataccctc
    10261 tctatctttt ccaactacag tgattccaac tattgttttc ttcattggta gagatgatta
    10321 atgattcctt atattcttag gtttttcgga aaataattga aatggtgcgg gaggtgggat
    10381 tcgaacccac gaactcctaa aagacagggt cctaaaccct gcgcctttga ccaggctggg
    10441 cgactcccgc aataattgtg caacaacaga caaatttatc gttattggat tgggtacatg
    10501 ggtaaactgt ttggaacaaa tggtgtaaga ggaatttttt ctgaagattt caatttagag
    10561 tttgttcacg atcttgtttt atcaatttca gcctatttta aacacggcac aatacttgta
    10621 ggatatgatg ggagacactc tagtcccaca atatcaaaaa cggtttgtga tacgttaaac
    10681 tccgctggtc ttgactgcca tctagcaggt ctagtaccta caccctgttt ggaatatgca
    10741 actaagaaac ttgggtatga tgggggaatc atgatcactg catcacataa ccctcctgaa
    10801 tataatggaa ttaagccagt tgccagtgac ggtgttgaga tttcacgtga tgacgagaaa
    10861 agtatcgaag agattttttt ccaaaaaaaa tggatggaaa ataattccgc tctgggttat
    10921 acgacaaacg attctagggc aattcaaaca tatcttgatg gaataaaatt tcaagtagat
    10981 gtagataaaa tcaaatctaa aaaattcaag gttgtattag accttggaaa tggagcacag
    11041 attattactg caggaaaatt atgcgatcaa cttggctgtg aggttattat gattaatgag
    11101 caaattgatg gaaatttttc tggtaggggt tcggagccta caccatataa tctagaagaa
    11161 ctttcttcta ctgttttgaa taataatgct gacattggaa ttgcttttga tggtgatggt
    11221 gatcgaagta tattttgtga taatgaagga aaattattat ccggagataa atcagcatta
    11281 ttactaacaa agtatctttt aaacaaaaat ccaaattcca aaattgttac ctgcttaaat
    11341 tctggaaatt caattgatga gattgttaca aatacaaatt cttcagtgat tagaacaaaa
    11401 gttggaagtg ttgaggtttc aagaaggatg attgttgaaa atgcactttt gggatttgag
    11461 gaaaatggtg ggttcatgtt tggaaaacat aatcatgtta gagatggagc catgacatta
    11521 gcattagttc ttgatttact tgctgattct ggtaaaacca taacagaaga actccagatt
    11581 ttaccatcct cattcaccac gaaagataaa atttcatgta caaatgaggc tgcaaatagg
    11641 gttatttctg aactaaaaag catgtttcca gatgctgata ttacagatgg gataaaaatc
    11701 gtatttgata agaaaaattg gattatggta agacaaagtg gaacggaacc aataatgaga
    11761 atttatggag aatctgattc acaaaaaaat cttgaggtat taatgagaac atatctagat
    11821 aaaatacaat taattctttc agataacact tttaaaacca cttgatacaa tttttaaaat
    11881 ggaaatgatc cctttgggtg atacaaatga ctaaaccgta ttatgtaaaa tttgaaacgc
    11941 ctgaaaattt ggtaagccct attttagaat cacttcgggt agctgccgag acaggaaaag
    12001 taagaaaagg cacaaatgaa gcaaccaaag ctattgaaag aggtataagt aaattagtca
    12061 ttattgctga agatgttgaa ccaccagaag ttgttgcaca tcttccaatt atttgtgagg
    12121 aacagaaagc acattatgca tttgttccaa gcaagcaaga cttgggtagt gcattaggta
    12181 ttgaggtaac cgcagcagct gctgtaattt tagatgcagg tgatgctcaa catataattg
    12241 atgaagtaat tgcctcgata gacaaaataa aaggtggcaa atcagacgaa tgagctcaac
    12301 agaacattca gacgaattaa ccactgctga agttattcaa attgtaggta gaactggtat
    12361 tgctggtgaa gtaatacaag taagagtaaa aattttagat ggtaaagatc gtggaagaat
    12421 attaacaaga aacgtaaagg gttctataag aatgtctgac atattaatat taagagaaac
    12481 tgaaagggaa gctaagaaga ttaggtagtg aaaataaatg agtatgttag taaaatcctg
    12541 cagtttttgt gaccgtccag tggcaaaggg ctctggtacc atgtttgtaa aaaatgatgg
    12601 aacagtaatg tggttttgtt ccgctaagtg caaaaagaac tctcttgttc taaaacgtga
    12661 tccaagaaaa ttaaagtgga caaaaaaata cgtgaaaggc ggaattagag tcaaaaaata
    12721 aattggctga acagacatta atcattataa aaccagatgc tgcaaagcga aatctggtgg
    12781 gagaaatcct ttcacgtttt gaaaaaaagg gatttactat ctcaaaaata aaggcattga
    12841 actttactgt agaaatggct gagcagtttt attctgctca taattctaaa ccattttttg
    12901 gagagttggt ttcatttatt acatctagga aagtcgtagc agcagtaatt gagggtgaaa
    12961 acgttatcga tacaactagg gaaattatag gtaaaactaa tcccaaggat gccgctcctg
    13021 gaactatccg tggagatttt ggaatagaca taactgaaaa ttctattcat gcgtcagatt
    13081 caagcgaaag ttttgataag gaagtgaatg tggtattcta gtgatcaaag atgcgcattc
    13141 gacaaccaat tgtgtccgtt ttgggccatg tagattctgg aaaaacatca ttacttgata
    13201 aagtacgtgg tactggtgta caaggaagag aagctggtgg tataactcag catatcggtg
    13261 caagttttct accggtagaa acaatacaaa aaatctgtgg accactttat gaaaaactga
    13321 gtaaggttga acaccaaatc cccggaattt tagtaataga tactccaggt catgaggttt
    13381 ttacaaattt gcgctcacga ggaggttctg cagcagatat tgctatttta gtcgttgacg
    13441 caaatagggg atttcaacca caaactaatg aaagtttaaa aattctagaa agccgtaaag
    13501 tgccttttgt tgtagcgtta aacaaaacag acatggtttc tggttggaag aatacagaaa
    13561 cacaatttat ttcacaatct attaaggaac aaaatgttga ggtccaaaac tttctagatg
    13621 agaaaatcta taatgttgtc acagcacttt ctgttttagg atatcagtca gaagcatttt
    13681 acaggattga tgattacaaa aaagagattg caatagttcc cgtcagtgca aaaacaggta
    13741 taggtatacc tgaattattt gcagttttag ttggattgac acaacaatat ctaaaagaga
    13801 aattaaacca agaggaaaag tccaaccgag gaataatatt agaagttaat gatgaagtag
    13861 gtcttggtcc cacagcaaat atgattttaa tagatggtca aatgagaaaa gaagattcta
    13921 tcatagcagc aaaaagagat tcagtaattg ttacaaaacc taaggctttg ttattaccaa
    13981 aacctcttga cgaaatgaga gatcctcgtg ataaatttaa accagtagag cacatacaag
    14041 cggctgcagg tgtaaaaatt gcatcaccgg atttagacgg tgttttgcct ggtagcacac
    14101 tttatgcagt aagtgatgat gcagagatag aacattttaa aaagattatt gaagatgaaa
    14161 tgcaatctgt tttcattagt acagaaacaa atggagttat tctgaaatgt gatgctattg
    14221 gatcacttga ggctataaca gaaatgttac gccgtcaaca aattcctatt tcaaaagctg
    14281 acataggacc tgtaacacat agagatattc tagaagctat ggcaacaaag gaaaacgata
    14341 ggcatttggg tgtaatttta gcatttaatg taaaggtttt acaagatgct gaaactgagg
    14401 cagataataa tcatatcaaa gttttcactg ataaaataat ctacagtcta attgatacat
    14461 atacgcaatg ggtagatgat gataaagcgg atgaagaaaa ttctgttttc aaagaattaa
    14521 caccgatatc taaatttact tttttgaaag gttttgtatt tagaaataac aatcctgcag
    14581 tgtttgggat cagagtagat gtaggtcaac ttcggtaaaa aattcctttc atgaataaaa
    14641 ctggaaaaaa aattggcatt atacatcaat tacaacatga tggtaaaacg gtaagcatgg
    14701 ttaagactgg acaggaagtg gcatgttctg tacaaaatat caccatagga agacaaatag
    14761 ctgaggaaga tgttttttac acattacctt cttcctcaga cgcaaaacaa ctgatcaaac
    14821 gattcatgca taaattaagt agtgaagaga cagatgtgtt aaatgaaatt atagagatcc
    14881 aacgagcaat tgatccagca tatgcatatt aaataaatta taaatcaaga agatatctca
    14941 tcaacacccc atcattttcc tttatctcca ttaaatgaaa tgtaggagat ttgatttcta
    15001 ccttgtagtt atgttttttt aggtttaatt tttcgccaga gatctttgaa acgatttgat
    15061 actccttatt ttttttaata ttgacggaaa aattttttat cgcaaacccg tcagttattg
    15121 ttatagtcac aatctcttct aaccaattat ataacaaatt atggatgttt tctccaacca
    15181 ctttgatata tttctcttca atttcttgta tattcttcga gtcaattata gtttcaacta
    15241 ccgatttacc agcaaccaaa aatgcttctt ccatggtttt tgctttgact tctataaacg
    15301 catcagttgc atgctcaagg tatttgtaac tcatgttttt tctatgaatt tctgttatta
    15361 atgagttaaa gtaaacagta agtcttaaag gcatttttta cgattttttt ttaaatggaa
    15421 ctaccacgtg gaatgaagga ttttgataaa aacgagatga tcaaaataga gttcatacgg
    15481 caaaaattct tggaaactgc aagtgtattt gggtttaatt taatggagcc ttcaccaatt
    15541 gaacttttat ctgtattaga agcaaaaagt ggaccttcta ttagagatga aatataccaa
    15601 tttcaagata agggtgatag ggaagttgcg ctcagatttg attttactgt tggattgacc
    15661 agatatgcaa cgtcacagaa aactttgaag ttacctgcaa agctttcttc atttggagga
    15721 gtttggagat atgacgaacc acaaaagggt aggtatagat tttttcacca gtggaacatt
    15781 gagatatttg gaaatctaaa ctcagattat gatgcggaga ttatagaatt cacatctaga
    15841 ttttttgata atcttactat gaaaaatata attattgata ttaatcacag aaaattggtt
    15901 caatcataca tcaatcaact ctttgaatca aacgaaactt ctcttttaaa tgatatattc
    15961 agagcagtag ataaaattca gaaaaaatca aaaaatgaaa tattacaaga atatactaaa
    16021 aagggttatt catcagaaaa attagaaaaa atattagaat tttcaaaaat taaaggtact
    16081 ccatcagaaa tccaacaaaa ttttgatgtt tcagatttag atggttggga tgaattagta
    16141 actttacatg attcattaaa acacagggga attgataata tcagaattaa ctttggaatt
    16201 gtaagaggtc ttgattatta ctctggaatt gtttttgaag catatgatac tacttctgac
    16261 ttaggtgcat tagtaggtgg tggaagatat gatagtttac caaacacatt tggccgtaat
    16321 gattttggtg ctacaggtgt ggcaggagga gtggaaagaa ttattctatg tcttgatgca
    16381 caaaatgttt cgtataccca atctcattac actgtatctg ttttgtattc aaatgaggaa
    16441 ttaaaattag aggcagttag aactgcatca aaactacgaa atcttggcat accaacaaat
    16501 attgatttaa atggaaaatc tttgaaaaaa caaatggaaa tatcatctag ctccaggttt
    16561 tctatcattt ttgcacctca agaattttca gagaagcaag ttgtattaag aaacatggtt
    16621 gatagaactg aaaaacgtat ttctttagaa gaattaatta caaatccaaa agatattctt
    16681 aatttataaa cgcactagtt aacatcatga tttcaggtcc attagttaat gtaccaacca
    16741 caatagcatt atcgttagct aatattccag atgaaacaaa tggaattcct ccattaatag
    16801 tagcaggttc aatcttaaca cctaagatat tagagaaagt attcacatca tcttcatctg
    16861 taagaggatg aattattcct ccattacttg tagctgacat tacagcacct acttgataaa
    16921 atcctgcaat ttttttctgt aacacttcta tatctaacac atcttcaatt tctttcacat
    16981 attttttatc aatagttgga gaaactattc ctcctttgtc attagcacag atcaagtttc
    17041 ctagagcatt atattttata tctaataatt ttatattcag atcagtgtgt ttctttaaat
    17101 gatcaatctc ttcctgtgaa catgtatttg gcaaaagcat tccatgatta tttaaaactg
    17161 caagaattcc aagcagtctg gtatttgcga ttgatatagt tagataatca gtttggaggt
    17221 agtttgctaa gttctctgct tttgtttttg cgaatccatt tggtacaaaa acaaattgat
    17281 tattgacttt ggtgtaaata ccaatatttg gaccgctgta aacatcatat ttgataatat
    17341 ccatatttga tacgtataat gaaaaaatat gaacgtaagg aatggaaaac agtgttatat
    17401 tattaaatta aaaaaaaatg gttcaggatc caatctagat ttatataata tctcataata
    17461 gaattcataa tgccaattaa agaagacttc tgtaaaggag acaaaaagcc aattggaaag
    17521 atcaattatt ctgacggtga aaattttcac tgggtatggc catctcaagc tggtgaagca
    17581 ggaaatgatt gggatgcatc tacagatgat aaagttctag ctgattacaa gaaacatggc
    17641 gaaaaaatgg agaaacttgg aatcactggt actatggtag caaatgattg ggatgtttgt
    17701 gtcgctgatg gtgcttgtat tgaagcatgt cctgtacaaa tatttcaatg gtacagaact
    17761 gataaggaca tatcaggcat agatgcagtg aaagataaaa ccccatggcc aggcgcaggt
    17821 acaaccgaaa aagaggaacg attagatttc acagataagg cagatgcaat aagagaacat
    17881 gactgtattt ggtgtatggc atgtgtatca gtttgccccc cattagcagt gttagtggat
    17941 caaggaaatc aagagtttca cgaaaaagct gcagggactt tccaagaatt aggttctgga
    18001 caagcaaatc cacacagtca ttagaaagca atttctaatc tattttttat tttttaaaac
    18061 agattttaat tagttaatat tatcaatttt tgcagaggtc gcctagcctg gtagggcgcg
    18121 ggccttgaga gcctgtgttc gtaagaacgc gggggttcaa atccctctct ctgcgttatt
    18181 ttttgctaaa ttttgttaat tcagctaata aagctgatag ttgaatttca gggttagcac
    18241 cagccaatat tctataatca tattttgcag tgatttctga catctctgct atgttatcat
    18301 tttttaaatt aaaaattgct tgattaatat attttaaaaa gtctgattca gacattccat
    18361 aaactttgat caattctatc attttttctc ttgattcaga aatctttcca tcaatcgcca
    18421 tttttaaaat attctggaca tcgtttgttt tagttaatcc aattactgat tttacactat
    18481 caacagtaac atttcccgta cttgctgatg cctgaagtat atttattgca tgtcgtaaat
    18541 caccatcagc ataagcagat atagccccca gacctttttc atctattttg atcttttcat
    18601 tttttccaat ttgttttaag tgtaataaaa tatctttttc agaaacctta gtaaatttga
    18661 atacaacaca tctactttgg ataggttcaa ttattttaga taaattattt gcaattaaaa
    18721 taaatctaca aaatttagct gtatcttcaa taatacgtct aagcgcagtt tgtgcatcag
    18781 atgtcatttc atccgcttca tcaagaatta ttattttgaa tggaattttt gtatctaatc
    18841 cagcaaacct agagaatttt tttacacgtt ctctgaccat gttaattcct ctttcatcag
    18901 atgcatttag ctcaagagta tagttcttcc aatattcacc caagatttct ttacatatac
    18961 aaagtgaaat tgtagttttg ccaactcctg ctgaaccaga gaataaaaga tgaggtaatt
    19021 ccagttgatt ttttagcatt gaatttagac taccaatagt ttctttttgg tttataagct
    19081 cagatatttt ctgcggtcta tatttttctg tccacatcac gtttgactgc attttcatat
    19141 ctcaagcagt cataccttat taataaatgg gttagaggat caaatgatca aagctgtttg
    19201 taaattcatt tgatcgatcg cacccattaa attcctcaac ggagatccat aaatgttgga
    19261 tatacaaaat cttgtacgtg ttcattcgat aggtaatgat ctacatgatg tgaaagttga
    19321 atttaacaaa gatttgaaaa tagatatttc agatataact gttgaaggta aacagggtga
    19381 gatcatcaac attcctagat ggattgcaga tatattacat tcagctaagc atgtggaaat
    19441 taaagacgaa gatatgttca gtgaattaaa acaatctatt gtgaaagaaa aggttcaagg
    19501 tgaatttgat ctttcaacct tggacgatta tttctatatc aaactgaaat catatatgaa
    19561 aagattatct gaaaatgatt atgataaagt agaaagtatg cttaacactt tgctaagaaa
    19621 aaggcagggt aagatagttc atctagcaga ttcatcaaaa ttaactcctg atatatcaca
    19681 gaaattatca atagaagaac gtgaattttt taatcaaata tatgaaatta gtacaaaatt
    19741 ttccaaaaaa atagttggtg ataaaaaatg actgcagaat cacaaacaaa atcagcaatg
    19801 tcagatcaag tgaaggaatt tttaacacag ttcaaagata ctactggatc tttttcatat
    19861 gttgaacaaa ttgatcaaat gatgtctaaa cgtatgacac acattatgat tgactataat
    19921 gatcttgttt catatccaga tattgaatca aaatttaatg aacgaccaga tgacatccta
    19981 aaagcgttct caaaagccat tggggagatt ctgcatgaac gttttccaca atactcagag
    20041 aagattaaaa aggtaatacg tgcacgtata gcaaattatc ctgtacaaag aagcctacgt
    20101 caaatcaaag ctgatgtcat aagtaaaatg acaagtgttt caggtatggt agtaagatca
    20161 tccgaagtaa aaccactggc acaagaacta actttcattt gtccagagga gcatattaca
    20221 gttgtaaacc tattccaagg aataagctta gactcacctg tacaatgctc aacccctaat
    20281 tgtaagcaca agaatttggg tatagtacca gaatcaagta gatttattga ttttcaaatt
    20341 gtccgtttac aagaattacc tgaagattta ccaccaggac aacttcctca ttacattgat
    20401 gttagtataa aacaagattt agtagataac gcacgtcctg gagatagaat cattctaact
    20461 ggtatcgtca gaattgaaca ggaacgtata tcgggtgtta gcaaaaacga gagtgctctt
    20521 tacagattac gaatagatgg taacaacgtt gaattcattg gaggtagggg agtaaaaagt
    20581 tcaagacggt cagagagaga agaaatatct gccgaagaag aaaagataat taaatccctt
    20641 gcaaaaaatc aggatattta ttcacgatta atagattcat tcgctcctca tattaaaggg
    20701 catgagataa ttaaagaagc aattctttta ttgatagttg gttctaatca acggctatta
    20761 actgatggga gtaagattag aggagatatc aatatttttc ttgttggaga cccaggtact
    20821 gcaaaaagtg agatgctaaa attctgttca agaattgctc ctagaggatt atatacatct
    20881 ggcagaggtt caacagctgc aggtcttacc gccgctgttg tacgtgatag tactggaatt
    20941 ttcatgttgg aagctggtgc tgtggtactt ggtgatcaag gtttggtttg tattgacgaa
    21001 tttgataaaa tgagagcaga agatagaagt gctttgcatg aagtgatgga acaacaatct
    21061 gcaagtatag caaagggtgg tatagtagct acacttaatg caagaacatc aattcttgca
    21121 gcagcaaacc caatgtttgg aaaatatgat caatttaaaa acatacatga aaatgtaaac
    21181 ttacctattc cacttcttac aagatttgat ttaattttta ttgtaagaga tatcccatca
    21241 caagtaaaag atagggagat tgctaaacac attatagatc tacatggtca agatggaaca
    21301 gatactaaat cactcataga tattgatata cttacaaaat atctagcata tgcaaaacgg
    21361 aaagagccta ccctaacagc tgatgcagaa aatacaatat tagaatatta tctgaagatg
    21421 agaaatgttg aatcagaaga catgctcact gtaactcctc gacaattaga aggattggtg
    21481 agattggcta ctgcacgctc aagactctta ctaaagaatc aagtagacaa agaagacgca
    21541 gaaagagcag ttttcatgat ggaatctatg ttacatgaaa caggaataga tgttaatact
    21601 ggcaaagttg atcttggtgt attacagggg cgtcctcgta gtgagatttc aagaattcag
    21661 acatttatgg atattttaaa ggatttagag ggtgaacaaa aaacaccagt agaagaacag
    21721 caattcatag accatcttgt aaaatcagag aaatttgatg aagaaggagc aaaaaattac
    21781 cttagaaaaa tgattcgtga aaacactatt tatgaatcta aacctggtca ttataaccgg
    21841 gtatgaatat ttctgaactt aacttaccaa aaaaatcaat tgatttttta cttgagcaag
    21901 gttatacaga actttatccg ccacaagaag atgccgtcaa ggcgggaatt attgatggaa
    21961 aaagtgtttt agtttctgca cctactgcca gtggaaaaac attaactgcg acaatggcaa
    22021 tattatctca cctgtcaaaa aacaaatcaa aagtaatcta tcttacacca ctacgagcat
    22081 tagcttctga aaaatttgaa gaatttaaaa aattagaaaa aatcaatcaa gaccaaaaga
    22141 tcaaagttgc aatatctact ggtgatttta attcaaacag tcatgatttg gatgatgcag
    22201 atgttatcat tcttacaaat gaaactatgg atgcaatgat gacattccaa aaatcttgga
    22261 tatatgaaat aggattaatg gtttctgatg agatccatct aattggtgat tctggaagag
    22321 gacctacact agaaatgatt ctaactcgat tcaaaactgg atatgttggg aaaaaaccac
    22381 agataattgc attaagtgcc actatatcaa attcagataa attagcagaa tggttagact
    22441 gtaaactggt tgagagtgaa tggagacctg taccactatc tgaggcagta tacagtaatc
    22501 atactataac aaatcaagat cgtgttgaat ctgagggtaa tcttgataga aatagagaat
    22561 ctagacataa gaaagctgaa attggtctag gattagatac tatagaagat caagctcaaa
    22621 gtttgatatt tactatgaca agagctagtt cagttgctac tgcaactgaa gctgggaaat
    22681 ttgtagtgaa acaattaaaa aaagatgaac ttgaaaaatt aattaaaatt tctaaaacaa
    22741 tccttccaaa agagactgaa gatcaaacaa aactagtaac aaaattagca gatatggtaa
    22801 aaaacggtgt tgcatttcat catgcaggac ttgatcaaag atgtcgtaca ataattgaaa
    22861 ctgaattcaa aaatggtcat atcaaattat taacctcaac tccaacttta gctgcaggtg
    22921 taaatctacc tgcacgccga gtgataatat caaacgtatt cagattcgga tcaaatggaa
    22981 atgcaccaat tactattttg gagtataaac agatgtgtgg aagagctggt aggccacagt
    23041 atgatgatga aggagaatca ataattattg caaaaggttc accacacgat tatctagagc
    23101 attatataga tggagaaccc gaatctcttg aatcacatat acttgaagat agttctctaa
    23161 gaattcattt actaggtctg attgcaacat catctacttt ttcttcagta tctgaaaaaa
    23221 agattaatga ttttttttct caaacttttg gtggactttc tgatactgca ttagaaaata
    23281 aaatatcaga acgtctaaaa gatctgaaag aatatgatat gattactgat gaagatggat
    23341 tcaaacctac aaaatttgga caaaaagttt tttggttaag aattgatcca aaaactgcat
    23401 caaacataac ttctgacctt gaacattatg ttaaaggaag caagcatacg tttggttcat
    23461 tacatatgat tactaatctc cctgaattct atccacattt tgacgttcca gaaaaacttc
    23521 aagagtatat ggaaataata tatgataatt ttaagcatga aaaattatat gcacaacaag
    23581 aattacagaa aaattggaca aaaagtctgc taatattata tcattggatt gatggtatga
    23641 cgtattctga tatgtctgaa aaatttgatg ctgaacctgg tgatatattc caaatacgtc
    23701 gaaatgcaga acagttggca tatattatta gagaaatcgc cagattttgg aaaaaccaag
    23761 tacttgttga cgaattggat attctcagac agcgcattcg tcatggcgtt cctgaagaat
    23821 atcttgatct agtcagaatc aaaaatgttg gtcgtgtgag ggcaaaaata ttatacaaat
    23881 ataatttccg tgatcgtata gatttgaagg aagtctctgt tgagaaatta gcagcaatag
    23941 ataaaattgg tatgactatt gcaaaaagta tcaagtcaca gatagaaaag gtaagataat
    24001 tgcaagaaca aataatcata gctataatcg tttcatccat tgcttttttt tcagtatatg
    24061 gcttaacccc gattttcatt aaggcattag caagcagaaa tcttacggta aaggacgtaa
    24121 acaaaaaagg cgatgttatg attccaagac cgggaggtat tcctatagtt attggacttg
    24181 ttgcagcaga aatcgccttg tatgcctttt tcccgatttc agagattttg gctgtgatta
    24241 ttgtgtctat tttgggattt tgtgtaggat ttgtagatga tagaaaagta atgggaggtt
    24301 ggttcaaacc tctcgcattg gcattttgtg catccccaat acttttgttg ggagcctatg
    24361 attcaaatct aagtttccca ttatttggtt tagttcaaat accatcgtta tatttggcgt
    24421 tagtagtttt tatgattccc attactggaa atacaattaa ctccattgat gttcttaacg
    24481 gtgtagctag tggtgttgtt acaatagcaa gttttgcatt agcaatttca ttatttatta
    24541 tgcaaaatta tgaaattgga atagcaagtt tatgtttggc ttttgcctct cttggatttt
    24601 ataaatatca taaatttcca agtaaaatat ttcctggaga ttcaggtgca ttggtattag
    24661 gttcaacata tggtgtaatt gccatagtag gtcatgtaga agtaatttct gcaattgcaa
    24721 ttttaccagc agtaatcaat tctttcttat ttcttactag tgttaaaaaa attgttgagc
    24781 atagggaaat taaaaatcca actttacgta ctgaagattt taaaataaaa tctacggatg
    24841 ataaaactgc acctattact cttgtaagac tacttgttgc aaaactaccg ttatcagaaa
    24901 aacaaattgg tgccgaaatt tttaaactat ccatattttg tggaatttta gcagtgataa
    24961 cagcattttt tatggaggta aaaatttgat atcagtaaaa atttatgctt tcatattttc
    25021 tatgtggatt ttaattttga ttggtggtgg tttaatggta ttattcattg gaccaatttc
    25081 atttcctgaa aatattgatc caaaaataac ttctgttgta aaaatatcac tagccctatt
    25141 tttgatattt atctgggttt tgatacttac aaaaattaag aattggatat ttaagtcaca
    25201 actagaatct tagattaaca cttcaagcat ttttccactc actctgcttt ttatcatatt
    25261 cttgccgtga gtattttgcc tttgcaggta caccaatgac tactgtgtcc ggaagaacat
    25321 cattggtaac gactgctccc attgcaacta cactgttttt acctatagta acaccagctt
    25381 ttatgaccgc ccgaccacca atgatgacat tatcctcaat tgttatcccg attaattttt
    25441 cactaggagg atatgggtca tttgttaatg tggcacttgg tccaatgaag acattttttc
    25501 caattctagt gagtggtgga atgtacacta atccctcaat aagcgtatta tctccaattt
    25561 ttacattata atcaacatgt gctaaggagc caatcttgac atcatttcca atttcaacat
    25621 tatctccaac ataagcaaaa tgccagatct ttacattttt gccaatctta gctttatcag
    25681 aaatatagtt agaaatcaaa attcctcaca agtgccatgg tagtgcttcc ttaactattt
    25741 tttgcggtaa ttgttcaatg catgcttgta gaaattcaag atcacgtttt ttatctctca
    25801 gatcatctgg taacatgatt ggaatttcat ctattatagg gaaaaatctc gaacattttt
    25861 tacagtataa cactccttct actatttttt cattttcagt tttacattca aaaagatcta
    25921 gcggatgaaa tttgtctata ggacatgcaa gtatttctaa tatttctctg ttcattttat
    25981 atcaagataa atgggtatcc ctttttgact agataggagt gcagcttcag caatttttgt
    26041 gacattaaca gcctgttctg ctttcacttt aatatcattt ttagtttcta ttgcacccag
    26101 aaaatttttt atctctaata ataacggctc tgttttttca ttttttggat tttcagtacc
    26161 atgatctgtc tcaatcctaa tttcttgagt aatgaaatca gaaaatattc ttccatcagt
    26221 acaaacagca ttaaaatttc tcacacgtgt tggagttatc caattagaag agatagtggc
    26281 agttttgttg tctttgaagc caagcattat tgtagcaaaa tcttcatgct catgattaat
    26341 tttacctgct atagcaaaaa caacttctgg tgttttacca aataaccaca tcgcggtatc
    26401 aatatcatgc accgcagtat cataaatgat accaacatct ttgatatgtt gtggcattct
    26461 atgctctcgg taaaattcaa gtctaatcaa atcaccatat ttttttgagg taagaaaatc
    26521 ttttactgta gaaacagcag ggttgaatct ttcaatataa ccacatgtta gaactacatt
    26581 gtttttatta gccatatcta caagtttttg accatcttcg gataaatacg tgaggggttt
    26641 ttcgacaaaa acatgctttt tactctcgat taattttgcg gctatatctg catgtgtgat
    26701 tgttggtgta cagataaacg cagcatcaaa ttcttctttt tctaataatg tttcaagtga
    26761 attataaaaa ttaacagaat atttttttcc aaattcttcc gcaaggtgca ggtctttatc
    26821 acaaactgca gaaagaacac caagttctga aagaactcta caatgattct taccccaacc
    26881 accagtacct atttgaataa ttttcaatca atacacccta gttaaccatt tagagtattt
    26941 tttgttttta ttcattacga tgtttgaata agtagtcatt aacaatttag taatgtttcc
    27001 aacttttcca gtgccaattt ttctcctatt tattttaata actggtgtaa tttctgcagc
    27061 agtaccagaa agaaaaacct catcagctaa attcaattca ttttttctaa tttttcttat
    27121 tactattttg atattcatat cctttaatat agtaataatt gattttcgtg taatcccatc
    27181 aagtgctgag gaacttaatg gtggagtaat cacttgatta ttttttacta gaaatatgtt
    27241 ttcaccagga gcttcactta catttccatg ttgatctaaa agaattgctt catcaaaccc
    27301 tttttgttta gcatcttgtg ttgccaaaat agaatttaga taattccctc ccatctttgc
    27361 ctgtgtagga gttgacatgt cactaaattt tctccacttt gatatagttg cagtgatacc
    27421 attcttgtta aataaatcac caaaatgaaa actaaatata gatacatgtg ttggtgcttt
    27481 ttttgtaaca tgcaaattaa ttccataatc accaacaaaa taaaatggtc taatgtaaca
    27541 tgattttttt attttatttt ttttacataa atttattatt gcattttcaa tttgtttatc
    27601 tgaaaaattt agtgtaatat cataaaattt tcctgaattt ctaaatcgtt taacatgttc
    27661 ttttaatcta aaaatatgta atttttctga attccaatat gctctaattc cttcaaatat
    27721 tgatgttcca taatgtatag catgtgttgt caccgggact ttagctttat ttgtaagtac
    27781 aaacttacca tcaaaccata catacttaga aaacaaaaat ttcacaaaaa tttgaaaaat
    27841 aatctataat taatctattt tgaattataa ccctcttttg gaaatttcat gcctgccact
    27901 ctagtagtca tatcttctcc ggaagcaaat tttgtgatta tatcccaatt ttcactaatt
    27961 atttgagcaa tattctcagg aacagatttt ttccattcca tatcattatt ttgaactgct
    28021 gcgtatatct gattttttac atcttttgca gagatgggtt ttctaatccc aacctttgga
    28081 tttaatctgt atagtttgag cataagacct tcagctttat ctcctgtata tgtaaaataa
    28141 tcactttcaa ttccttgtaa aatttgtttt tttaatgccc atgattttgg agaaattaat
    28201 ggtggaaaat attttttaaa aggagcatga aaagaatagt ttgatgaaac gcttacagtg
    28261 tcaccaaata ctgattcaat catttttttt cttatttcaa aattaaatgg aaaactttta
    28321 gtattaattt cctgttcttc ttttaaaaaa cgtacaggca ttacaactac cttattttct
    28381 tgttttaact cctcaataat ttccacatga gcgtttgtaa ctggattcaa atgtgcaaga
    28441 tatattgcaa tactcaatgt gtatactcaa cggtggtcat atttcaatga ttgatttttg
    28501 ataagaatga tgggatttga atccatgatc tccagtacct aggtcgacag tatatcaaac
    28561 taacccatgt ccctataaga taatcagagt gaacagtatt attttctagc tatttatcca
    28621 taagattcga atttaacaga aaagtttcct tttttgtctt tttctttttc taataaaaat
    28681 ccttttggtc ttacgtaatc caaaacacca tcaactgttc cctgccaacc acatacataa
    28741 aatatagtgt tatcaggagt aaccttttct ccaacaagct cctcaagagg agattttcca
    28801 ctgctagttg gttttaaaaa ttgttcaacc cttccagttt gaccagtcca tgatctattg
    28861 aaccattctt gtggcctact aatagctgct ctatatttaa aattccattt gttagaacct
    28921 ctatccaaac tttcttgatc taatgctgtt aattcttcct tatagcttaa ttcatcaaca
    28981 taactagatc catgaaggca tacaagctct cttttatcac ccaaatcatg aagatgtcta
    29041 gcaaagctca tgaatggagc aatacctgtt ccaccaccta tacaaacaat tctcctttga
    29101 tccttactac cgtcacccat cttctcatta atttttaatg cagcaccagt tggtggaatc
    29161 caggaaactt catcaccttc accagcatta aataatgcag ttgttaccct accaggtaat
    29221 ggtcttctaa cccatctaac taccaattca atgaattttt tattttcagg atgtgaagca
    29281 attgagtatg ctctccgaat aattttatta tcttcacttg gtattggcat tccaactgtg
    29341 atgaattgcc ctgcttcata atttggaatt acaccatctg ttggaacaag tctaaaaact
    29401 gctaaatctt cttttagtaa ttgaacatat gtgataatag cttttttgtc gattgccata
    29461 ggtgttgatc cgcttcgtta tggttaaaaa tattgtgatg aatcttaggc taacctaaag
    29521 tattactcgt ttagtaatcc taaaaggtcg ataaattatg gtttatctat gggccggtcg
    29581 ttcagcctgg taggatgcgc gcatggcatg cgtgaggtca agggttcaaa tcccttccgg
    29641 tccattcgac attttttcat atttttcaag tagaaatatt aattcttcat ctgcattctc
    29701 actcagtttt tcaatttcat tgtcaaaaat tttacctcct atcttaaaaa acgcttgaac
    29761 tgcatttttc ctcacttcaa tttttttatc atcaaaacag tttctgatga gatttattga
    29821 ttgccgagca tttagatgtg ccaatgcact aagagcatag gatctcacca tactattagt
    29881 atcctgtatc aatctttcaa catgtggtat tgcattggaa tctcctctat ttgctaaaac
    29941 aagagacaaa aaacctttaa cattgttact ttctgaattc aaaccatcaa tcaaaatatc
    30001 tgatatgtta ttttcattaa gaaaaagtga ataaaatgct tcacccctta cctggatatt
    30061 ctgatcatct agttttgata tgattttttt gataatctcc ggattttgtg tgtgtatgtg
    30121 ttctgagatt tcagttttat ctatttttga ttcatccatg atgaattaat tatagatagt
    30181 aagatgtaaa aatattctga atattattca tatttttaga tctgatttaa attacagcta
    30241 atgctcaggg cagtatgtca accgacaaca cagaaacaaa cacagaaaca aacacagaaa
    30301 caaacacaga aacaaaaaca acaaccaata cttctcagcc ttctgtaaaa actgaaccaa
    30361 gaagacaaga tgttgtatac atcggaaaga aaccattgat ggcatatgtc acatccacat
    30421 taattcagtt ggcaaatgtt aaatctgtta gcattgtcgc acgcggtatg agtattagtc
    30481 gtgctgtaga tgttgcacaa atcatatcac gcaaaacaga gcatactgga tatagtattg
    30541 gagatatcaa aattgattct gtaccaatgg aatcaaaaga tggaagaact agaaatgttt
    30601 ccactattga aattaaagta gagagaaaat aactctactt gttttctttt attttttata
    30661 tttttgttct aattttctat attttgataa ttccacaata tcattgaatt gattaaagtt
    30721 tacgatttgt ttagataatt ttctagttgt tcctgatttt tttaattctt gcagcaattt
    30781 gagtgttgaa tatgtattgg aaaacaaaac tgaaagtgga tatagaacta ttttgaatcc
    30841 cattttgtgt aattttgatt tagaaattac tggtgttgct ccaccctcta tcatgtttgc
    30901 taccaatggg gcattaattg attttccaat tattttcatt tcttgaatgg tttttggtgc
    30961 ttcaatgaat atagcatctg caccaatttt cttataatac aatcctcttt caatagcttt
    31021 gtctaatcct tctattgctc gtgcatcagt tcgtgccaca ataataaaat ccttattact
    31081 tcttgcgtct aatgcagctt gaagtttttc agcatattca tccttttgta taacttcttt
    31141 tccttgcata tgaccacatc ttttgggcca tctttgatcc tctaagaata taccagacgc
    31201 tccaacttct tctaattctt gaactagttt ccatatactt agtgcatttc cataccctgt
    31261 atccgcatct acaatgactg gtgttgagac agcatgacaa atccttcttg cattgtcaac
    31321 agtttcagtt gaaccaataa atccgtaatc aggcattcca aacaatgtag ctgatgttcc
    31381 ataaccagtt tggaacatgg cttcaaaacc aactttttct actatctttg caccaatggc
    31441 atcataaaca ccaggcatta ccaacggctt tgatttatcc tgaaggagtt tcctcacact
    31501 tttcataatt catatttttt ttgaattatt tatggattag tctatcttga tcttttgctc
    31561 tctctcatta gacatttttt ccattttatc tagttcagat ttcaagaatt tctcatattt
    31621 tttaatctct tctttagtca tctgtgctgc attctcactt agtaatgaac caataacaga
    31681 tagacgctct agcatatcta atatgacaaa tctacctaca ctaccaagcc taaacagaac
    31741 atccaattga ttttttacaa tctcgttaat tgtttgaagg tcatcagtag tttttctacc
    31801 tttttttgtt attctatact ttccatcttt atcttcagaa attaacccat catctagaag
    31861 ccttcctaac aagggataaa ttaaacctgg agagggtttc cattttccgc cactctgttt
    31921 gatagctgaa tcaatgattt gttttccagt atatgatttt tttttcaata attcgagtat
    31981 aaaaaaacgt gaaaaacctc ttggtattga acttccaact cgttgaatcc agtcacctat
    32041 catcactagt gatatcacta gtgatatata taatactttt gtgggttgta aataatagag
    32101 atcaagattt ttagaattta catacatatt gacccaatca aaatatcaga ttaggaatga
    32161 aaaatagtct tgaatatgat ctgataattg ttggttctgg tcttgctgga ttgagagcaa
    32221 ctattcaagc tgcaagaaga aattccaaaa tacggattgg agtaatatcg aaagttcaag
    32281 ttatgcgttc tcattctgtt tctgctgaag gaggaactgc agcggtattg tttccagatg
    32341 aaggtgattc tattgaatca catatttatg atactgtaaa gggtagtgat tttttggcag
    32401 atcaagatgc tgcagaacga ctttgcaaag aaatacctaa cgaaatctat caacttgaac
    32461 attggggtat gccatggtcc agacgagagg atggtaagat tggccaacga gcttttgggg
    32521 gttatagttt tcctcgagca tcatatgctt cagataaagt tggatttttt gaaatgcaaa
    32581 cattatatga tacatgccaa aaatttgata atatagaatt tcttaatgaa tggtttgtaa
    32641 cttcaattgt tcaaagtgga aaaaaatttg cgggtataac agcaattgag ttatcaagtg
    32701 gcaattttta cacaatcaaa ggtaaggcgt tgataattgc aactggtggt gctggtagaa
    32761 tttacagttt ctcaacgtat gcactttcat caacccctga tggtttagac atggcatatc
    32821 gtgcaggcct tggtttaaaa gatatggaat ttgtacaatt ccatccaact ggaatcatgc
    32881 cttctggaat attgataaca gagggtgcaa gaggggaagg tggtcatctg ctcaataagg
    32941 atggtgaaag attcatggca aaatatgcat caaaattgat ggaattagca ccacgagata
    33001 tagtatctag agcaattatg actgaaatta atgaaggtcg tgggttcaaa aatgaaacag
    33061 gagtagattg tatgaaactt gatcttcgac atataggaga tgaagttatt aaacaaaaac
    33121 ttggcggtat tagagaaata tcaataaaat tttctggtgt agatcctgca aaagaattat
    33181 tggacattcg acctgtatgt cattatatga tgggtggtat tcataccgac ataaatggtg
    33241 cttcagaatt aaaaggagta tgggcagctg gagaggccgc atgtattagc atacatggtt
    33301 caaacagatt aggtgcaaat tccacttcag aatgtatagt ttggggaaaa attacggctg
    33361 acgaggctgt cacttacata gaaaaggaat caagctcaga atcatttcct gatcatttag
    33421 tttatgcgga agaaagaaga atatatgatg gaattttcag gggaaaagga gaagttaatc
    33481 catatgaaat aaaacaaaaa cttaccgata cgttaactga gaaagcatat gtttttagaa
    33541 atgaaacaga tttggttgaa gggttaaaga aagttcgtga actcaaggca atgacatgga
    33601 aacatgttga tgataaggct aaagaataca acactaattt ttcaaacgtt atggaattag
    33661 actcgatgtt tagaattgct gaagtagttc taattggggc aatcaataga aaagaatcta
    33721 gaggttctca tgcaagaact gatttcccaa agagagatga taaaaatttc ttgcatcata
    33781 cattagcata ttatgatcct caggagccaa ttatgaaaac tcatccagtc ataataacta
    33841 attacaaacc tgttgagagg aaatactaat ggaaaaagat cgtaaggagg gcatcaaagg
    33901 aatggcaaat cccggacgat atgggataga acgtgtagca tattggttga tgagaataac
    33961 tggtcttgga ttgttatttt atttcattgg tcatatttat gaaacaagta atctgcttga
    34021 tggaaaagca gcttggaatt ctatgttaga attaacacaa accacagagg gtcacatatt
    34081 cttgacgcta gttattggaa tgtgtgtatt tcatacaggt aatggaatta gattgatgat
    34141 tggtcaagca ggatttgggc tcggtaaacc tagtagagct gattatccat atacaccaaa
    34201 atcacttaat atgaaaggta aactttgcat ttatgtttcg attggtctcg ctgcattagc
    34261 tatgatgtat ggtctaggag tgatgtatga tgtctaatat aatgcgtgaa agtcacataa
    34321 tgaagattca ttatttgact gctctaatag ctgttggatt tgtaattatt catattatgg
    34381 ttagagttac acaagggttc gcaaattccc tagaatttga gagtgtgatt gcaaactaca
    34441 aatctattcc ctatgctata gtgttagaag ctatgctaat actcatttca gtccatgggt
    34501 tcaatggttt acgtataatc ttacttgagc taaagcaagg aaatgcatat gaaaatgcag
    34561 ttacatacgg gtgtttggtt gcaatgattg ctcttattgc atatggttct agaacaataa
    34621 ttatggcaag catgggaatg gtggaagcat gactaaagat ggagcattac caaaaggatt
    34681 tgaaagacca agtagcttac cctcacaaga aaaatacgaa aaagtaaaat catcttccaa
    34741 aacaacaact cttagaatat ctaaatttaa tccggaaagt gattctactt ctacattctc
    34801 agaattcaaa gtgcattatc agagatggac tactgtattg gatgcaattt tggaagttaa
    34861 aagtaactat gatcattcaa taggtgtacg ttattcatgt agacaagcta catgtggttc
    34921 ttgtggtatg attattaacg gaaaaccaag acttgcatgt tttacaaaga tatctgaatt
    34981 agattctgat ttggtaacag tggaaccaat gaataacttt ccaataattc gtgatttagc
    35041 tgttggattt gaaaggctca tagcaactca taagcgaata aaaccataca taattcgtga
    35101 tgattcggaa gtaacttctg aaaccaagga gtttttacaa tctcccgagg atgttgaaaa
    35161 atatattcaa tttgctggat gtataaaatg tggtttatgt aactctgcat gtccaacgat
    35221 ggcaacagat tcatcctttg ttggaccaca agcgctagca caagcctata gatatgttgc
    35281 agataataga gataagggta aagacgaacg gcttaaaatt attgatgaac ctcatggtat
    35341 ttggagatgt cactttgctg ggtcatgcag ccaagtatgt ccaaagggag ttgatccagc
    35401 aatgggaatt caactactaa gaggatattt gttagggtat agaaaataat tattcttcag
    35461 atgatttaga agactcgtct tcggtttttg tttctttttt gtgtggattt gggctattat
    35521 ttacctgatt attaatctgt tcagccatga aatggtttga aacgtttact ttgacatcat
    35581 caattccatc aatactcaaa agatcatcat gtatatcttg acagatttta aacccaaata
    35641 cagctggaca aaaggggcta gtaagatgca aatcaacctt gacattagta tcctgaatat
    35701 caacttcgtc aattaattca agttctgtga ttgatgtatt aatttcaggg tcaacaatgc
    35761 ccgataattt atcaaagatt ttaactctta tttgtttaag ctcttgagac attaacgaca
    35821 aatcatttat gctatatata gtcattctga cttgtatatt caatgtaccg ttcacgctta
    35881 ggaaaaaatt tgggaaagat aacattagat tatgtatcat cactaaatga tgacattcaa
    35941 attgtaatgt atgatattct aggaagtcaa gcacacgtta tcatgcttta tgaaaataaa
    36001 attatcgtaa aaaaggatct aaaaaagatt ctaactggac ttgaattttt aaaaaaagaa
    36061 aattttcgaa agaaatctag ctctgaagat attcacgaat taatagaatc attactaatc
    36121 aaaaagatag gaattgatgc aggaggaaaa atgcatactg ctaggtcacg aaatgatcaa
    36181 gtagcattag atattcgtat gaaaattcgt gatgacacaa atattatttg tcaatgtttg
    36241 ttagatacta taaaatcact tgttaaatta gctaaaaaac atcaagaaac tattgttcca
    36301 ctttacacgc atcttcaaca agctcaagtt ggtttgttct ctcattatct tttatcttat
    36361 gctgatgctt tgttcaggga tcttgatagg ttttacacaa catatgatag gataaatgaa
    36421 aatccattag gagctggacc aattggtggt acaagcataa tgattgatag acaaagtact
    36481 ggaaaaatgc taggattcaa tggcattgtt gaaaattcaa tcgatgcaac aaccactagg
    36541 gattttgcaa ttgaatatat tgcggcaagt gcaatattaa tgacgaattt aagtaggatt
    36601 tcaaaagatt tttctatttg gtctacttca gaattttctt tcttggaact atctgatgaa
    36661 ttcacttcac catcaagtgt tatgccacaa aagaaaaatc cagacatcct tgaattgact
    36721 cgtggcaaaa catcccagat aataggtaat ttaacatcta tactatcaac aatacaagta
    36781 ctgccatcag gttatagtcg ggatttacaa caggttaacc cgtcactctg ggatacatct
    36841 catactgtaa tttctgctct attgattatg gagtcaatgc tgaatacaat tatcataaat
    36901 aaaaaaaata tgcaaaaggc cgcggaggat ggatatctta tagctcttga tctagctgaa
    36961 aagttagttc accatgacat atcatttagg aaatcacatc aaattatcgg tatactagtc
    37021 aaggctgcgc acaaatcacg taaatctatt tctgctttga caaaattaga ggtaaaaaat
    37081 gtgatgaagg gggaaaaagt gaatttggaa cttttgatga aattgataca atctactaca
    37141 gtcatgtctt cactcaacga caggaagtct caaggttcat ctggaatatc agagcaaaaa
    37201 cgaatggtaa tacacagaat gaaaaaaatt gatatctatc aaaaggaagt aaaaaaacga
    37261 agtaataaca tacaaaactc gctagatagc ctgaataaaa aagtcaaaac attaatcaaa
    37321 taaaacgggt ctagtgggat tcgaacccac gacagccgga ttaaaagtcc ggtgcgctac
    37381 ctggctgcgc tatagacccc gccaagatac attaactgta taatttagtc ttttacagat
    37441 tatcggtagc tggaaacttt aaaattgata actttgagcg aatagttgag cccttgtagt
    37501 atagcccggt ctagaattcg gccctgtcac ggctgagacg cgtgttcaaa tcccgccgag
    37561 ggcgccatta ttttattaac gaaaaaacct cactcaaaat ttctttattt gtcgccgcta
    37621 caaatgacag tctagtttca taattaagat ctgagtcaag cggttctagg ttttcatcta
    37681 atattaggcc accagcctct ttaatcaaaa gatatccagc tgcaatatct tgaaccctaa
    37741 tctttcccct caaatcaata aaaatatcaa ttaatccttg agcaaacaaa gccatctcta
    37801 gggcatttgc accaaaatgt ctaacatgat gatgtttttc ataaatcgga gaaagtcgtt
    37861 tgagtaattc tggagaagta ccagaactat ttattccaac aatcttgtaa agtggttttt
    37921 ctttatggac agaaattggt ttatcattta gaaaagcacc tttttctttt gatgtccaat
    37981 aaatatcacc tgttgaaaga tttgtaatcg cagcatcagt aatggaactc aatttgtcat
    38041 cagttgcaaa tgcaagtgag cagcaaaaaa atggtacacc tcttacagca ttagcacttc
    38101 catctattgc atccattatc acaaacccct taggatttga tgataattcc actttcccac
    38161 attcttctcc aagtactgta cattcaaaat ttatttcttt gaaataatca acaacagttt
    38221 tttctgcaat aatatcgata tttcgtgaaa tatctccacc tgcacctctt ccaaaatctc
    38281 cagccactgc ttcttcggta ccaggtagat ctttgacatt ttcataaaca agtttagaag
    38341 cagatctaag aatttcaatt atatccataa agaatttcag ataaaaacat aatttaagtg
    38401 aaattttttt taatgcataa ataccaattt acaaatgtca agatatgagc ggtaaggatg
    38461 aatctgttac tagtaaaagt tccctgaaag gaaccaaagc tggaaagaag ataattaagc
    38521 aatatttttt caaatctaag gggtataggc agttcaataa atacaaaaag gaatatgaaa
    38581 caaactttcc tgaattcgct aaacgattca caaatgacct tttgcaacaa attaaagatg
    38641 attcatctcc taatattacg cagcagaagt ttggggaaga aattggttca actgatatca
    38701 ttttaggatc ttcagaaatt gatccaatca aatcaaaact ggaaagcttt gaaatactta
    38761 atgatagagt tctaagaatt ttaaattcaa attttgtaaa aatgaccttt cctgttttct
    38821 atggattatt tgatgcatct gcagagtatt ttcatgatag caatgatcca aaacttagag
    38881 aagatattat tgatgggcat attattgcaa ttgatctcag tgaaccgatg gatagaatca
    38941 tggacagaga tgaagattta gattatcttg atgactataa actgatgaat ccatatatac
    39001 taaaattagc tcgtgataaa attgcaaaag gcggggaaga agttttgaaa caatttgaat
    39061 ctggattcag agatgctaga attggacagt atattgatgc aacgttaaaa caaaatccta
    39121 cttctatgac agaagaaaaa ctcgatgaga gttataagaa atatagatcc gtaatgggta
    39181 cggccggttg taatatggca ctttcaagac aacctcttgg agaaatattt cagataggaa
    39241 tgggtaaagc ttcagaatca gttgggtgtg gcaatgaaat tgaagattca atacgtgata
    39301 aggcagtaaa aatcccatca tggcctttgt attacagttt attagaaaat gatgttagaa
    39361 agggatttga tttgaccatg aaaaaaagtg aatcgtatct tcgtgaagca agaaacgcac
    39421 ttgaaagttt accaaaaaac ttttctcaca gagaattttt agaattttta tttctcacag
    39481 tggagcatta taatgaattt tggttcaata gattacaaca cgaaaacatt tggtcagatc
    39541 tccagtcaaa tcttccaaag tgatttttat gatgtggtca gaaaaatatc gccctaaaaa
    39601 cattattgat ttgattggaa atgaggaggt taggtctgat tttgtcgatt ggtttactaa
    39661 atggaaaaag ggtacaaaac caatcttatt agttggaccc cctggcatcg ggaaaactac
    39721 acttgccaaa ttagcagcga agcaatttgg atatgattta attgaactta atgcaagtga
    39781 tgtgcgtagc aaaggcagga ttcaaaatat acttcagccg atcttaggca gtgaatcttt
    39841 actggggcat ccaatgattt tcatcgatga agtcgatgga atacatggaa gagcagatta
    39901 tggaggagct gaaaccctca tcaaaattct aaaagaacca accgtaccaa ttttactcgc
    39961 agcaaattct gatatttcta ctaaaatgaa aagtatcaaa aaagccgtaa gaacaataca
    40021 cctaaaaccc ttatccccac gaatgatgca attatacaca aacattatac taaaaaaaga
    40081 gggagtatca ctaacaccaa aatctctgca gaaaatagta attgaatcaa gaggggattt
    40141 acgttctatg atcaatagca tacaagctaa tgtgacaggc tttgagccac caacggaaaa
    40201 atcatttgag agattagaca tagaagaagg aattaataca ttttttaaat ctcattcaat
    40261 cgacgaagct agaatgatac tttattctat gcatattgat cctcgtgaga agattaatgc
    40321 attttactct agcatcataa caagcaatat tgataaaaag aatctcgcgt atatgttaga
    40381 aataatatct aaagctgata tgttatatgg aaagataatg aaaacacaaa actggagatt
    40441 gttaagatat cttgattcta ttttattagg actttacaaa cctaatacat caattcgtta
    40501 ttcacggttt aatctatctt ggcctatgat aaacagaatt agatgggatg gaaaaaagat
    40561 taaactcttg tcgaaaataa tatccaagaa tttacatatt tccgtaagca catttacaac
    40621 atttgttttt aatactatgt tgttctgcat gaaaaatgat aagctagatt ttgaattaga
    40681 tgaggaattt gatgatatta ttgaaaaaga aatgagtttg ataaaatgag ctggataaaa
    40741 attactatga aatatgatgg aacctgtatt gtatgcaata aaaagatcag agcaaaagag
    40801 attggttttt ggctaaaaga agaaagtaaa gttaaacatg aaaaatgtgc tgaagtaaat
    40861 aaagaactga aatgtgctat atgtaatggc tcagctgggt gtatggattg cgaatttgct
    40921 gaaatatgtg atcgtgaaaa tgtttccaaa ctttgtatct gtaaaaaatg tgagagtgta
    40981 catgattcgt ttactttata tcaaaaatca attattgaaa aatttcctct tctaaatctt
    41041 aaaatttgac cgattttaag cactcttaat gtcagaaggc aaaaaagaag aaactcagaa
    41101 agaaacaggt ccagtaacac tttcagaggc agaggcagag gcagcagtag caaaagcaga
    41161 atatgaagct gcaaaggaag aggcagaaga tgcaaaaccc gatgatggta cgcttagatc
    41221 aacaacaatt tcagatgctg tagcggcatc ggagttagca gccacagcag aaacagcata
    41281 tgaagcagca aaagaaaagg taaaagctgc aagaatagca tcagaagccg catggaaagt
    41341 agagtatgaa gcagcaaaag ctaatgctgg tgaaataaca aaagatgaat ttactcataa
    41401 acggaagcag gaccggatat ttagaagaga aatgggtgaa ccagaatttt ttggaaatgc
    41461 aaagaatatt ccattaaaag caatgcatac acaacaagag gaagaagaaa tttcaagaaa
    41521 agtacagata ccaacagatc aaaccccaaa atataatcca gaaaatttat tgagtcctga
    41581 atttgctgga atttctaatt atgaatccgg gcttggtgat attttagatg aatcaaaact
    41641 gaaattagaa cgacttctaa atgatccaaa ttcaactgta caagaaattg aacttgctaa
    41701 aactaatcta aaaaaattaa attatttatt tgaaaactat catttgggaa tgaatgtctt
    41761 tagaacagct aaaggtggta gggataaaat taaggaatag actgatgtga catggctaaa
    41821 aataaattta atattttaaa tgcaagaatt acttttaaga atgtccctat acacaaaatc
    41881 acaaattatt catttaaaga catttcaact gcatctgaat cctttaagaa aatcaatgga
    41941 gtctcagaat gtataataat tcaaactgct agtagagtgg aaatttttct agttagtaaa
    42001 tcagaatact ttgcagtcga aaaaataaaa gaagtctgga catcactaac agaacttgaa
    42061 caaattgaca ttgatcattg ggatcaaact ttagaagtgt atatgaatac tgatgtttac
    42121 cgtaatctgt tacgattagc atctgggata gaatcagtgg ttgttggaaa ggaagaaata
    42181 ttggatgaaa ttaaaaatat gtcttcaagt gctaaacaag ctggaaactc cggtaaagta
    42241 ctggataaat tatttgacag ttctataaga attgcaactg atatcagaaa ttcaaccgga
    42301 ataggtattg gtataaaatc aataggtgat atagctgtta atctagcgga agaaaaggtt
    42361 ggaaatattg gttccaaaaa aatattgctt ataggaacag gagaaactgc agctatggtt
    42421 gctaaatgtt tcaataaaca aaatcatggg tttgatgttg cgagtatgtc tatagaacgt
    42481 gcaactggtt tttcaaaaat tttaggtgga aacccagtaa actttgaaga tgtgatagga
    42541 tttccaaaat atgatatagt tttcgttgca actacagcag actattttct agtctctgct
    42601 aaagatatga aaaaatcaat gaaaaagaga aaatctggta ttatgatatt agatctatca
    42661 gacccaagag cagttgaatt acaggtgggt atgattccta aaattaaggc attgtttcgg
    42721 gacgaaatat ctgaattaga tgatgaaagt ggtaccagga gaaagaagac ttcaactgta
    42781 gaagaagcca ttagtaaaga agttccaatc cttgaagaat caatgaaaca acttaagcag
    42841 gggaatatca ttacagccaa ttaactattc ataatatgaa agaataagat attcattaat
    42901 ataataaaaa taatcttgtt tgttagtaaa tatgcattct gataaaatta atgaattttt
    42961 aaaaaaaaga aagggatcta gtcacgctgg tggtcaggat aaaatttcta aacaacatga
    43021 aaaagggaaa ttaactgcga gagaacgagt aaatctttta cttgatgaag gaagttttgt
    43081 agagattgat gcgttaacca cacaccatta ttatgaatat gatatgcaga aaaagaaatt
    43141 ccatggagat ggtgtgatcg gtggatacgg aagtgttaat ggacgacaaa cttacgtttt
    43201 cgcttatgac tttacagttt tgggaggaac actgagtcaa atgggtgcaa aaaaaattac
    43261 taaattaatg gatcatgcta ttcgtaatgg ttgtccaatt ataggaatta tggattcagg
    43321 tggagcacga attcaggaag gtatactaag tcttgatgga ttcggtgata ttttttatca
    43381 taatcaatta gcatcgggtg ttatcccaca aatcactgct agtattggac catctgcagg
    43441 tggtgcagtt tattctccgg caatgactga ttttgttgta atggtaaaca aattaggaac
    43501 tatgtttgtt acaggacctg atgttgtaaa gacagttctt ggtgaagaag tttcatttga
    43561 tgaattgggt ggagcaatga cacatggtag taaaagtggt gttgcacatt ttgttgttca
    43621 gaatgaatac gaatgtatgg atcatatcaa gacattgtta tcatatattc ctcaaaataa
    43681 tattgaggaa cccccaattg tagtaaacgg tgatgatcca aatagactgg atcataatat
    43741 aattaacttt gtacctgaag atcccgtaaa accgtatgac attaaaaaaa ttatttattc
    43801 aatagtagat gatcataact tttttgaaat tcatgaatta tttgcacaaa atattgttgt
    43861 tggatttgct agaatgcatg gaagaacaat tggagttatt gccaaccaac cattgtttct
    43921 tgcaggtgca cttgatattg attcttcaaa taaagcagca cggtttatca gattttgtga
    43981 ttgttacaat attccaattg ttacattggt tgatacacct ggttatatgc cagggaccaa
    44041 tcaggagcat aatggaatca ttcgacatgg cagtaaactt ctttacgctt attctgaggc
    44101 aacagttcca aaaattacta ttgtaattgg aaaagcatat ggtggtgctt acattgcaat
    44161 gggtagtaaa aacctaagaa ctgatattaa ttatgcttgg ccaacagcac aaattgcagt
    44221 tctagggtct gaaggagccg ttaagattat gaatagaaag gaacttgcga attcaaaaga
    44281 tcccgagtca ttgaaaaaac aactggtaga tgaatttact gagaaatttg ctaatccata
    44341 tgttgctgca tctcatggaa cagttgatac agttatagat cctgcagaaa ctagacctat
    44401 gattataaaa gctcttgaaa tgctttcaaa taaacgtgat aagcaattac ccaaaaaaca
    44461 tggaaatatc aacctgtgat caaaatgata agcaaagtac taattgcaaa tcgtggagaa
    44521 attgccttac gtgtaatcaa aacttgtaag gctctaggaa taaagacagt tgcagtatat
    44581 tctgatgaag atgtacattc acttcatgta aaaaatgcaa cagaagcata tcatattggt
    44641 gaggctgctc ctgcaaagag ctatcttaat caagaaaaaa tccttgaagt catgttatct
    44701 tctggtgcag atgctgtaca tcctggttat ggtttccttt cagaaaatga tgactttgca
    44761 cgattatgtg aaaagaacaa aatcaacttt attggacctt ctgcagattc aatgaatctt
    44821 tgtggagata agatgcgttg taaagaagca atgctcaaag ctaaggttcc aactgtacct
    44881 ggaagtccag gtttagtaaa agatgctgat gaggcagaaa agatcgcaaa tgaaattggc
    44941 tatcctgtaa tgttaaaatc agtttatggt ggtggtggtc gtggaattag aattgtaaac
    45001 accgatcaag agttacaaga tggatatgaa acagttacag cagagtcaat tgcagcagta
    45061 ggaaaatctg caatcattgt tgaaaaattc ctagaaaaaa caagacatat tgaatatcaa
    45121 atgtgtagag atcatcatgg aaatacagta catctctttg aaagagaatg ctcaattcaa
    45181 agacgaaatc aaaaactaat tgaacaaacc ccatctcctg tggttgatga agccaaaaga
    45241 gaagaaatcg gtgaattagt tgtcaaagca gcagaagctg ttgattatac taacttaggt
    45301 actgcagaat ttctcagagc agattctgga gaattttatt tcatagaaat taatgcaaga
    45361 ttgcaggtag aacacccaat ttctgaaatg gtatctggat tagactttgt taaacttcaa
    45421 ctagatattg caaatggaga accattacca ttcaaacagt ctgatcttaa gatgaatggc
    45481 tatgctattg aatgtagaat taatgcagaa gatactttct tagactttgc accatcaaca
    45541 ggtcctgttc cagacgttac aattccttca ggaccaaatg ttagatgtga cacatacttg
    45601 tatcctggat gtactgtttc accattttat gattcactaa tggcaaaact tgtaacatgg
    45661 ggacgaacat tcgacgaatc tagacttaga atgcttaatg cgttaaatga tttttacatt
    45721 cagggtgtgg aaacttcaat ccctctttac aaaacaattt tgaacacaga agaatacaaa
    45781 aatggaaaac tcaccacaga ttttttgaaa cgatataata ttattgaaag attggaagaa
    45841 gacataaaaa atcaaaggaa tgataaacaa attgaagcag ttgcctctgc cgtaatgtat
    45901 tcagaattct ttcaaagtaa aataaaatca tcaaatacga caaactcact atggaaaagc
    45961 ttaatggatt gatataaaat ggaattcaaa cttgaagatg tagaagaaac tttcaacgga
    46021 gaaattatta ataaaatttc aaataatgaa tatttaatta agattcaaaa taaagaacat
    46081 caacttcaaa tattaaatat taattctaac gggatggaat ttgtattaga taataaattc
    46141 cataatgtga aatatgtgga aaataatact gctgaaatga ggatcgttat tgatggtgta
    46201 tctttgataa tcaatatgca
//
LOCUS       DASCPJ010000018         1769 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000289265, whole genome shotgun sequence.
ACCESSION   DASCPJ010000018 DASCPJ010000000
VERSION     DASCPJ010000018.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000289265"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="QGF01_01200"
     CDS             <1..277
                     /locus_tag="QGF01_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HJN19550.1"
                     /translation="PNLSPANYNDFLDVGINDWGGISPITVDYVNPEFAWPQINTLEN
                     KCTEQSFELKARFPVYPKFIKMINPNLKEKIEKLSDDEKYVRREYWR"
     gene            274..1527
                     /gene="cofH"
                     /locus_tag="QGF01_01205"
     CDS             274..1527
                     /gene="cofH"
                     /locus_tag="QGF01_01205"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HJN19551.1"
                     /translation="MTLETKNFDSKLKHADPIIAGILNDALSEKEIDIKDSVMLFSAK
                     GIELELVCSVADELRKRRVGDVVTYVVNRNINFTNVCIKQCGFCAFSRDFREEEGYLL
                     PIEEIVRRAKEAHELGATEVCIQAGLPPDMDGDLYEKICREIKKEIPKMHIHGFSPEE
                     ILYAATTNDITIRDYLLRLKNAGVDTIPGTSAEILDQKMRDKISPGRISVKDWIKVIK
                     TAHKIGVRSTSTMMYGHMESYVDRANHIGIIRKIQKETGGFTEFVPLNFIHAEAPMYK
                     HDLHKGINPGADSDDIMLTHAVSRIMLNNCIDNIQMSWVKTGEKMSQQLLRCGANDFG
                     GTLINESISTAAGSQHGQLLKPKQIRRLVRDVGRIPAERNTTYKILRTFENEPNDEDL
                     DNVDDSKFGSYFDLIKIKKFRYERT"
     gene            complement(1524..>1769)
                     /locus_tag="QGF01_01210"
     CDS             complement(1524..>1769)
                     /locus_tag="QGF01_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HJN19552.1"
                     /translation="HTDIGIGTIVGSNIYNILMIMGVASVITGITVVPGMFTDYLVMI
                     GFAVVLVAFLRSGIIPRPAGVGLAIAYAAYLGYTLLL"
BASE COUNT          628 a          316 c          371 g          454 t
ORIGIN      
        1 accaaacctc tcacctgcta attataatga ctttcttgac gttggaataa atgattgggg
       61 aggaatttca ccaataactg tggattatgt taatccagaa tttgcttggc cacaaatcaa
      121 cactttggaa aataaatgca ctgaacagag ttttgaatta aaggcacggt ttccagtata
      181 tccaaagttc atcaagatga ttaatcctaa cctaaaggag aaaatcgaaa aactttccga
      241 tgatgaaaaa tatgttagga gggaatattg gagatgactt tggaaactaa aaattttgac
      301 tcgaaactta aacatgcaga tccaatcatt gctggaatac taaacgatgc attatcagaa
      361 aaggaaattg acataaaaga ttctgtaatg ttattttccg caaaaggaat tgagcttgaa
      421 cttgtttgtt ccgtagctga cgaacttaga aaaagaagag taggagatgt tgtcacatat
      481 gttgtaaaca ggaacatcaa tttcacaaat gtctgtataa agcaatgtgg attttgtgca
      541 ttcagcaggg atttcaggga agaagaaggt tatctcctcc caatagaaga aatagttaga
      601 agagccaagg aagcacatga attaggtgcg acggaagttt gcatacaagc tggtctccca
      661 cctgatatgg atggagatct atacgaaaaa atttgccgtg agataaagaa agaaattcca
      721 aagatgcata ttcatggatt ttcacctgaa gagattttgt atgcggcaac aacaaacgac
      781 atcaccataa gagattacct tttgagattg aaaaatgcag gagtggatac gattccaggt
      841 acgtcagctg aaattttaga tcaaaaaatg cgtgataaaa tttcgccggg caggatttct
      901 gttaaggact ggattaaggt tatcaaaact gctcataaga ttggggtaag gtcaacgtca
      961 actatgatgt atggtcatat ggaaagttat gtagaccgtg ccaaccacat tgggataatt
     1021 cgcaaaatac agaaagagac tggaggattt accgaatttg tgccactcaa cttcatacat
     1081 gcagaggcgc ccatgtacaa gcatgatttg cacaagggaa ttaatcctgg tgctgattca
     1141 gacgacataa tgctgacaca tgcggtttcc agaatcatgt tgaacaactg catagacaac
     1201 atacagatgt catgggtaaa aacgggtgaa aaaatgtcac agcaactact gagatgtggc
     1261 gcaaatgact ttggtggaac actgatcaat gaaagcatct caactgctgc aggttcacag
     1321 catggtcaac tgttgaagcc aaagcaaatc cgacgccttg tcagagatgt tggtaggatt
     1381 cccgcggaaa gaaacaccac ctacaaaatt ctcaggacgt ttgaaaatga accaaacgac
     1441 gaggatttgg acaatgtaga tgattcaaag tttggttctt attttgatct gatcaaaatt
     1501 aaaaaattca gatacgaaag aacctagagc aacaaagtgt aaccaagata cgctgcatat
     1561 gcaattgcca gtcccactcc agcaggtctg ggaattatac cggatcttaa aaatgcaact
     1621 aggacaacgg caaatccaat catcaccaaa taatcagtga acatgcctgg cacaacagtt
     1681 atgccggtta tcacagaagc aacgcccata atcataagta tattgtaaat attacttcca
     1741 acaattgtgc caattcctat gtctgtatg
//
LOCUS       DASCPJ010000019         7626 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000325172, whole genome shotgun sequence.
ACCESSION   DASCPJ010000019 DASCPJ010000000
VERSION     DASCPJ010000019.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000325172"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="QGF01_01215"
     CDS             <1..190
                     /locus_tag="QGF01_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiS"
                     /protein_id="HJN19553.1"
                     /translation="GKKTSLNKLHTELKPDINSKLLSRNNVSYIQKQFGISKKQISVI
                     SSKNPLEDILVERAAILF"
     gene            complement(182..700)
                     /locus_tag="QGF01_01220"
     CDS             complement(182..700)
                     /locus_tag="QGF01_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HJN19554.1"
                     /translation="MNLVVTCGRNLESEAEIEIKKILEANTCEGSEVLKTGMRGILTV
                     QTKMDPIKFVDYVKKKINEEPWSIRYCLRIIPIQHVTVTDLIEIKQSVEDLKDIIQKE
                     DSYRITVENRNSDISSMEVISEIAKIIPNKVSLENPDWVIQIEIFGNKTGVSILKQNT
                     IFSLEKAKRNLE"
     gene            complement(697..1296)
                     /locus_tag="QGF01_01225"
     CDS             complement(697..1296)
                     /locus_tag="QGF01_01225"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HJN19555.1"
                     /translation="MRISCDNIDMQSAYQTVKQGGIVIFPTDTVYGIGCDPYNENSVS
                     LLYKIKKREKTKPFPVLGYSKKELEKIAEFNSLATKIAEKFWPGPITMILNVKDTDIQ
                     KSLGLGGKIAVRVPYNTCALSLLKECKVLVGTSANISDETSLIDPNECETKIPNYDLL
                     IDGGIIISKGESTIVEIDGDKAKIVREGSVSEKEIYELN"
     gene            complement(1327..1611)
                     /locus_tag="QGF01_01230"
     CDS             complement(1327..1611)
                     /locus_tag="QGF01_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJN19556.1"
                     /translation="MPSHRTQMKIVGEILSTTMDTDSQDGTNITHLIRMANISHGRIS
                     TILGNLVSQGLLEQVDSKRSCKYKISSSGKEFLLAYKTFREFSEDFGLTI"
     gene            complement(1705..2358)
                     /locus_tag="QGF01_01235"
     CDS             complement(1705..2358)
                     /locus_tag="QGF01_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19557.1"
                     /translation="MEFLFEIILNLIGFCVGVIIGVISYIGFKNTGSPTLFRLTIAFF
                     AIGIGFGVLALGYIIDDTITHTGNVNRGITTIGIAIQTIGYWFIAFSHTIKTFFPKSK
                     YLRSVGVLPLFLVSFASVEHVIRAVSFILLVYGSIETMLSYLQGRKKSTLFVAIGLGL
                     LGLGEFVSWYSFVFPESILYAISIVIKIGGLTSLGIPISRIPLQKVKFDPELKDDST"
     gene            complement(2425..2634)
                     /locus_tag="QGF01_01240"
     CDS             complement(2425..2634)
                     /locus_tag="QGF01_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19558.1"
                     /translation="MKIICPKCKGTAELTPDFSLVICKKCDYEISYGEYIKHVAHSES
                     TYSDILGDYTGNTEGQTAGSLEEWD"
     gene            2710..2988
                     /locus_tag="QGF01_01245"
     CDS             2710..2988
                     /locus_tag="QGF01_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HJN19559.1"
                     /translation="MSRVCTKCKSEIPDNIELEPLQSTYPCCPKCWDEWKEYRVMVMN
                     EMRLDMSLKDHRKLLKKNEKVFLGVMTPEGDVIDYSDEKNRKPEEPPA"
     gene            complement(2985..3446)
                     /locus_tag="QGF01_01250"
     CDS             complement(2985..3446)
                     /locus_tag="QGF01_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19560.1"
                     /translation="MTEPNYAGNIIVILANLPDFLRKPILKKRMSEFYSMSDQEKNIL
                     IQNALEAGPTIPFPNFSKLFKTWLEVLADETEEHRQILISGYVNEITNFPQKIIAFNL
                     DGMLEIYFTLEDSKKMIINNSLKLIIGKLESDAKRKIILLIPNVLKNELGV"
     gene            3523..4524
                     /locus_tag="QGF01_01255"
     CDS             3523..4524
                     /locus_tag="QGF01_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HJN19561.1"
                     /translation="MPSTVVVGGFFGDEGKGKVISYLAKKDNPSIVVRGGAGPNAGHT
                     IKDGDTTYKVRMLPSGFLNKNARVMIGPGVVVNPDVFFKEIQEYGVSDRAFLDKHCGI
                     IEQNHLDQDSKGRLKEKIGSTGSGTGPANAERAMRTLKMAKDIESLSSHVIDVPAEVN
                     SALKDQKNVLVEGTQGTHLSLWHGTYPFVTSKDVTASGICADIGIGPKRVDEVLVVFK
                     AYLTRVGTGPMAGELDAKETEQKGWAEFGTVTGRPRRAAEFDFPLAQRAVMLNSATQI
                     AITKLDVRFPECAGIKSFNDLNDDAKSFIKNIEDKLEVPVTLIGTGPLLDDVIDIRE"
     gene            4570..5235
                     /locus_tag="QGF01_01260"
     CDS             4570..5235
                     /locus_tag="QGF01_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19562.1"
                     /translation="MDKFPNYIRVTSPLEFTRLVCALERSPRVSFLHEHEGKKVLSVQ
                     MDMLKESPVIYYTPVESFDHYISYGFRSGKEESVMVNSTLDNSKLYSPIVKIKSLPRS
                     LRPSTNSSSIKYQPLEFEDLGSLAKLSFGFEEAPFPLFSFPFNGKWLLGVFLNFNEDG
                     DSFFCYVTLKEEPTKPFLKHTTTSGGQPVFVDNTSEHGYSYIKIVKLQETHPLVNYDQ
                     IQS"
     gene            5216..5953
                     /gene="pyrF"
                     /locus_tag="QGF01_01265"
     CDS             5216..5953
                     /gene="pyrF"
                     /locus_tag="QGF01_01265"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HJN19563.1"
                     /translation="MTKFKVRIHKSSVVSRIILANDYDLGTEKLVSKTLGNIKTLSEY
                     ICAIKLNFHTLLPLGKKEITRINKTAHDYGLQTIADIKLNDIGNTNLVTTKTLWSLGF
                     DAVIANPIMGLDALSKIVKAAHNNNNGVIALCHMSSPEAKLSYDMSVKLSNSKLTSLY
                     NVFLKWAISSKTDGIIVGATFPKIIKECKKAIGRKMDIYSPGVGVQGGNPKQTIESGS
                     DFLIVGRTILNSKNPVQTAKKLQLASI"
     gene            complement(5950..6357)
                     /locus_tag="QGF01_01270"
     CDS             complement(5950..6357)
                     /locus_tag="QGF01_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HJN19564.1"
                     /translation="MVKQKSAGIVLFQNSHGANHFLLLNYPQGHWDFVKGKMEHDETP
                     HDTARREAGEETGITDIEFIDDFEESVEYEFRFNGDLIQKKVIFFLAKTNTSKISLSH
                     EHKDFVWLEYEDAMEKITFQNAKKILAKANNFC"
     gene            complement(6389..7099)
                     /gene="uppS"
                     /locus_tag="QGF01_01275"
     CDS             complement(6389..7099)
                     /gene="uppS"
                     /locus_tag="QGF01_01275"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HJN19565.1"
                     /translation="MGDMPNHIAIILDGNRRWAKRNLVISKHGHFRGANAVENLLDWC
                     EEFDIKITTLYVLSAENLDRKNEELEFLYDLIKTRLEKLYDDPRIHKNQMRVKAIGRI
                     ELLPDSIKDVLSRLDNATKDYDKHFLNIAIAYGGQNEMVDAIKKIGSKIKSGELDTKD
                     ISKDVIESNLYTSHLPHPSADMILRTSGEKRMSGFLMWQSAYSELVFLDIFWPEFRKI
                     DLMRAIRIFQKRKRRIGK"
     gene            complement(7256..>7626)
                     /locus_tag="QGF01_01280"
     CDS             complement(7256..>7626)
                     /locus_tag="QGF01_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19566.1"
                     /translation="STVYTSGNVEIAQLLTNDVVVGQFVSGMIPFLWIGLGTMFAGIL
                     FSNWLNRKINHVTDTLRIIVLISLYPTVHQFTNILVNDASSFSLILPLVIGFGVTAAS
                     ATLLIRRYRKKKYEKQSESE"
BASE COUNT         2554 a         1298 c         1173 g         2601 t
ORIGIN      
        1 aggtaaaaaa accagtctaa ataaacttca tactgaactt aaacctgata ttaactcaaa
       61 gctactttca aggaacaacg tatcatatat tcaaaaacaa tttgggatat caaaaaaaca
      121 aatatctgtg atttcttcaa aaaacccatt agaggatatt cttgtagaaa gagcagctat
      181 actattctaa attacgtttt gccttttcaa gactaaatat tgtattttgt ttaagaattg
      241 acacacctgt tttattgcca aatatctcaa tttgtattac ccagtcagga ttttccaatg
      301 aaactttatt tggaataatt tttgctatct cagaaattac ttccattgat gaaatatctg
      361 aatttctatt ttcaacagtt attctgtagg aatcctcttt ctgaataata tctttcaaat
      421 cttctacact ttgttttatt tcgataaggt cagttacggt aacgtgttga attggtatta
      481 ttcttaggca gtatcttatg gaccatggct cttcatttat tttctttttg acataatcaa
      541 caaattttat cggatccatt tttgtttgaa ccgttaaaat acctctcatt cctgttttga
      601 gtacctctga gccttcacaa gtattggctt ctaaaatttt ctttatttca atttctgctt
      661 ctgattcaag atttcttcca catgtaacaa ctaaattcaa tttaattcat agatctcctt
      721 ttctgagaca ctaccttctc taacaatttt tgctttgtcc ccatcaattt caacaattgt
      781 agattcccct ttactaataa taattccacc atctatcaat agatcataat ttgggatttt
      841 tgtttcacat tcattgggat caattaatga tgtttcatcg gaaatattcg cacttgtacc
      901 caccaacact ttacattctt tgagaagtga taaggcacat gtattataag gaactctaac
      961 agcaattttt ccccccaaac ccaaggattt ttgtatatca gtatctttta cattcaaaat
     1021 catagtaatt ggtccaggcc aaaatttttc tgcgatttta gtagcaagag aattaaattc
     1081 agcaattttt tccagctctt tttttgaata acctaataca gggaatggtt ttgttttctc
     1141 tctttttttg attttataca ataatgatac tgagttttca ttatatggat cacatccaat
     1201 accataaact gtatccgtag gaaaaatcac aatcccacct tgtttgactg tttgatatgc
     1261 agattgcata tctatattat cgcatgaaat tctcaagatg ctacaaaaca gataatacga
     1321 ttctaactaa atagttaatc caaaatcctc tgagaattct cgaaatgttt tatacgctag
     1381 aagaaattcc tttcctgatg aactaatttt atatttacag gatcttttgg aatcaacttg
     1441 ctctaagagc ccttgtgata ctaaattacc cagaattgtt gaaattctac catgtgaaat
     1501 atttgccatt ctgattaggt gtgtaatgtt agtaccatct tgactatcag tatccatcgt
     1561 tgtactaagt atttcaccaa caattttcat ctgtgttcta tgtgatggca taattattta
     1621 cacatatttt cagtattgat actgaatcat ttttttcaat atactataaa gacaaaagaa
     1681 attgacaaca acaaattctc atgattatgt actatcatcc tttagttctg ggtcaaattt
     1741 caccttctgc aatggaattc gtgatattgg aataccaagt gaggttaatc ctccaatttt
     1801 tatcacaatt gagattgcat ataaaattga ttctggaaat acaaatgaat accagctaac
     1861 aaattcacct aaccctaaca aacccaaacc tattgcgaca aaaagcgttg atttttttct
     1921 tccctgtaaa tatgataaca tggtttcaat tgaaccatat accagtaaaa tgaatgatac
     1981 cgctcgtatt acatgttcaa cagaagcgaa cgataccaaa aataatggta atactcctac
     2041 cgaacgaaga tactttgatt tagggaagaa agttttgatc gtgtgagaaa atgcaataaa
     2101 ccaataccca atagtttgta tagcaattcc aattgttgta atacctctat taacattacc
     2161 agtatgcgtt attgtatcat caatgatata tccaagcgcc aaaactccaa aaccaattcc
     2221 aattgcaaaa aaagctatag tcagccgaaa cagtgttgga cttccagtat ttttgaatcc
     2281 aatatatgaa ataactccaa ttattacacc cacacaaaaa cctatcaaat tcagaataat
     2341 ttcaaataaa aattccaatg ttgctaactt gttagctgtt ttaaataagg tttgagaaca
     2401 agttttctga ctttatgatc aacatcaatc ccattcctct aaactacctg ctgtttgacc
     2461 ttctgtatta cctgtataat caccaagtat atcggaatat gtagattctg agtgtgcaac
     2521 atgcttgata tattcaccat agctaatttc ataatcacat tttttacaaa taactagaga
     2581 aaaatctggt gttaattctg cggttccttt gcattttggg catataattt tcatacacta
     2641 gatttgaatt cttatctatt attggtattg atttgaaaaa aataaaagta actagttaat
     2701 gggaaaagta tgtctcgtgt atgtactaag tgtaaaagtg aaattccaga taatatagaa
     2761 ttagaaccac ttcaatctac atacccttgc tgtcccaaat gctgggatga atggaaggaa
     2821 tatcgtgtca tggtgatgaa tgaaatgaga ttagatatgt cactaaaaga tcatagaaaa
     2881 ctgttaaaaa agaacgaaaa ggtgttttta ggtgtcatga cacctgaagg agatgtcatt
     2941 gattatagtg atgaaaaaaa tagaaagcca gaagagccac cagcctaaac ccctaactca
     3001 ttttttaaca catttggaat tagaagtata atctttcgtt ttgcatcact ctccaatttt
     3061 ccaataatca gttttaagga attgtttata atcatttttt ttgaatcttc taatgtgaag
     3121 taaatttcaa gcataccatc tagattaaag gcaattattt tttgaggaaa attagtgatc
     3181 tcattaacat aaccagaaat aagaatttgt ctatgttctt ccgtttcgtc tgctaaaact
     3241 tcaagccatg ttttgaaaag ttttgaaaaa ttgggaaaag gaattgtagg accagcctca
     3301 agtgcatttt gtatcagaat atttttttct tgatctgaca tcgaataaaa ttctgacatt
     3361 ctttttttta atattggttt tctgagaaag tcaggcaaat ttgctagaat cacaataata
     3421 ttacctgcat aatttggttc tgtcaacaca atcccttcaa tttgtatgta ataaccgtac
     3481 ttgtaagttg aattaaatat gtgaattctt tagtatctct gtatgccttc aactgttgta
     3541 gtaggtggtt tttttggaga tgaaggaaag ggtaaagtta tttcttattt agcaaaaaag
     3601 gataacccgt caattgttgt tcgtggggga gcaggaccaa atgcaggaca taccatcaaa
     3661 gatggtgata cgacctataa ggtacgtatg ttgcctagtg gatttttaaa taaaaatgct
     3721 agagtgatga ttggacctgg tgtggttgtt aatcctgatg tcttttttaa agaaatacaa
     3781 gagtatggtg tttcagacag agcatttttg gataaacatt gtgggataat tgaacaaaac
     3841 catcttgacc aggattctaa gggtagatta aaagaaaaaa ttggaagtac tggctcagga
     3901 actggtcccg caaatgccga gcgcgcaatg agaactttaa aaatggcaaa agatattgaa
     3961 tcactttctt ctcacgttat tgatgtacca gcagaggtta attctgcgtt aaaggatcaa
     4021 aaaaatgttc tagtggaagg cactcagggt acacatctat cattatggca tggaacttat
     4081 ccatttgtaa catccaagga cgttactgca tctggcatct gtgcagatat tggtatagga
     4141 ccaaaacgtg tggatgaagt tttagttgtg tttaaggcat acttgactcg tgttggaact
     4201 ggtcctatgg ctggtgaact tgatgctaaa gaaaccgagc aaaaaggatg ggctgagttt
     4261 ggtacagtta caggtaggcc taggcgtgcg gcagaatttg actttccttt agcacaaagg
     4321 gcagtcatgc tgaatagtgc aactcaaatt gctattacaa agttagatgt aagatttcct
     4381 gaatgtgcag gcataaaatc tttcaacgac ttgaatgatg atgctaaatc tttcataaaa
     4441 aatattgaag ataaacttga ggtcccagtc acgcttattg gaactggtcc actcctagat
     4501 gatgtaatag atattcgaga gtaatatttt ttggttaacc tttaatgtag tattttcttg
     4561 atatctaata tggataaatt tccaaattat atcagagtga catcaccttt agaatttact
     4621 agattagtat gtgcattaga aagatcacca cgtgtatctt tccttcatga acatgaagga
     4681 aagaaagttc tttcagtaca aatggatatg ctaaaagaaa gtccagttat ttattatact
     4741 ccagtagaaa gctttgatca ttacatttct tacggtttta gatctggtaa agaagagtct
     4801 gttatggtaa actctacatt agataattct aaactatact caccaattgt aaaaataaaa
     4861 tcattaccac gatctctgag accatcaaca aatagcagta gtattaaata ccaaccactt
     4921 gaatttgagg atctaggaag tcttgcaaaa ctcagttttg ggtttgaaga agcaccattt
     4981 ccattatttt cattcccatt taatggaaaa tggttacttg gagtttttct gaattttaat
     5041 gaggatggtg actcgttttt ctgttatgtt acattaaaag aagagccaac aaaaccattt
     5101 ttgaagcata caacaactag tggtggacag ccagtttttg tcgataatac cagtgaacac
     5161 ggttattcat acatcaaaat agttaaacta caggaaacac atccattggt caactatgac
     5221 caaattcaaa gttagaattc ataaatcatc agtagttagt cggatcattc ttgctaatga
     5281 ttatgatctg ggaactgaaa aacttgtttc aaaaacccta ggaaatatca aaacattatc
     5341 agaatacata tgtgcaataa aactaaactt tcatacactc ttaccgttgg gaaaaaaaga
     5401 aattacaaga atcaataaaa ctgcacatga ttatggatta caaacaattg ctgatataaa
     5461 actaaatgat ataggaaata caaatttggt aactacaaag acattatgga gtttaggatt
     5521 tgatgcggtc atagctaatc caattatggg tcttgatgca ttatcaaaaa ttgttaaggc
     5581 tgcacacaat aataacaatg gagtaattgc attatgccac atgagttcac ctgaagccaa
     5641 attatcttat gatatgagtg taaaattatc taactctaaa ctcacatcat tatacaacgt
     5701 attcctaaaa tgggcaatat ctagtaaaac tgatgggata attgtaggtg caacttttcc
     5761 caaaatcatc aaagaatgta agaaagcaat aggtaggaaa atggatattt attcaccagg
     5821 tgtgggtgtt caaggtggta atcccaaaca aactattgaa agtggaagtg actttcttat
     5881 tgtgggaaga acaattctta attcaaaaaa ccctgtacaa acagctaaga aattacaatt
     5941 agcaagtatt tagcaaaaat tatttgcttt cgctagtatt tttttagcat tttgaaatgt
     6001 aattttttcc atggcatctt catattccag ccaaacaaaa tccttatgct catgagataa
     6061 ggaaatttta gatgtgtttg ttttcgcaag aaaaaaaata actttctttt gaattagatc
     6121 accattaaat ctaaattcgt attctacaga ttcttcaaaa tcatctataa actcaatatc
     6181 agttattcca gtttcttctc cagcctctct tcttgcagta tcatgaggtg tttcatcatg
     6241 ttccattttc cctttaacaa aatcccaatg tccttgagga taatttagta atagaaaatg
     6301 atttgcccca tgagaatttt gaaataaaac aattccagca gatttctgct ttaccatatt
     6361 ctgttatctt ttattccatt tatttaggtt attttcctat acgtcttttt cttttctgga
     6421 atatacgtat agcacgcatg agatcaattt ttctaaattc tggccaaaat atatctaaaa
     6481 aaaccaattc gctgtatgca ctttgccaca ttaaaaatcc actcattctt ttttctccag
     6541 atgttcttaa aatcatatca gctgatgggt ggggtaagtg cgaagtataa agatttgatt
     6601 caataacatc tttgcttata tcttttgtat ctaattcacc tgattttatc ttagaaccaa
     6661 tttttttaat tgcatcaacc atttcatttt gaccaccata cgcaattgca atattgagaa
     6721 agtgcttatc ataatctttt gttgcattat ctaatcttga taacacatct tttatcgaat
     6781 ccggcaataa ttcaattcgt cctatcgcct tgactctcat ttggttttta tgaattctgg
     6841 gatcatcgta gagtttttct aaccgtgttt taatcaagtc atatagaaat tcaagttctt
     6901 cgttttttct atccaaattt tcagctgata acacatacag agttgttatt tttatgtcaa
     6961 attcctcaca ccaatcaaga agattttcaa cagcattcgc acctctaaaa tgaccatgtt
     7021 ttgatatgac caaatttcgt ttcgcccatc tacgattacc atcaagaatt atggcaatat
     7081 gattaggcat atcccccata ctgatttctt tctccaatct tttagagtaa aatctgtata
     7141 gaccagaaaa attcaatact gcgtttataa taccattaaa ttcaatcacc catttctatt
     7201 tttaatgggt agccacagat atgttttatt acaaaaatgt tctaaagtaa catatttact
     7261 ctgactcgga ctgtttttca tatttctttt tcctatatct gcgaattaat agagttgcag
     7321 atgcagctgt tactccaaaa ccaattacca aaggcaagat taaagaaaat gaacttgcat
     7381 cgttgacaag tatgttcgta aattggtgta cagttgggta caaagaaatt aagacaatta
     7441 ttctaagtgt atcagtaaca tgatttattt ttctatttag ccaattactg aataagattc
     7501 cagcaaacat tgtcccaaga ccaatccaca aaaatggaat catacctgaa acaaattgac
     7561 cgactactac atcgtttgta agtagctgag caatctctac attgccagat gtatatactg
     7621 tactta
//
LOCUS       DASCPJ010000020         2458 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000334109, whole genome shotgun sequence.
ACCESSION   DASCPJ010000020 DASCPJ010000000
VERSION     DASCPJ010000020.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000334109"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="QGF01_01285"
     CDS             <1..537
                     /locus_tag="QGF01_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN19567.1"
                     /translation="ELKKMRESGLNMLYLGIETGSDIILKKITKGATSETIIKACKKA
                     METGFTLSCIIILGLGGKTYTKEHIEGTARVVNAASPHYLGTLTLILETGVKEEFLTK
                     FGEEFYPINDDEALIELHDLVQQIDIKEKMVFRANHGSNAYNIAGTFPDEKNAMLEKI
                     TWLKEHPENIRPEGFRAF"
     gene            complement(538..612)
                     /locus_tag="QGF01_01290"
     tRNA            complement(538..612)
                     /locus_tag="QGF01_01290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(575..577),aa:Val,seq:tac)
     gene            745..2160
                     /locus_tag="QGF01_01295"
     CDS             745..2160
                     /locus_tag="QGF01_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HJN19568.1"
                     /translation="MNSVVIAKFGGSVIGIDGASIPLIIERIKSLTGKSKVIAVFSAP
                     LTAVDGNRRSLTDVALELGKIAEDGSVPDLTIVRKTYEKISELVDSKFQNECKMMIDD
                     YLEKVKTELEKVVKNKEFADETRSKTLAFSGEIIMSQMMEYILKSHNIKSQFVGLEDW
                     PIITDNNIESTNFLVNESSKNIEHIEKMLEKNDVISIGGFIGRTKDGIITTYERGGSD
                     RTAADLGILLHKKFDVKIDLEKDRSVVSADPKIVSDGLTEVSSLSYNEARLAGMFGMN
                     ILDPIAIKEILENGASLPIIITDIKNPDKCTEVARKISAGNGHPIKIVTGKKNCAILK
                     IEEETVGELLKSLENEKRYSEFVELSPFIKDGIKFARMLFLDGDYVKRNEKYVLGFDA
                     LATITYNRGVITLIGDEMWRVQQIVSKTSAKIGEDGLNILNIDAQEETSRIIIVIEDS
                     ADNIKKAINAVHSERSNIKFV"
     gene            2199..>2458
                     /locus_tag="QGF01_01300"
     CDS             2199..>2458
                     /locus_tag="QGF01_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HJN19569.1"
                     /translation="MKETGKIWMNGKLIPFKDAKVHVLTHALHYSTAVFEGIRCYNTP
                     DGSAIFRLPEHVDRFFNSAKMYGMKMRYSKKQISNAIIKTVKT"
BASE COUNT          926 a          347 c          502 g          683 t
ORIGIN      
        1 gaattgaaaa aaatgcgtga atctggtttg aatatgttat atcttggaat agaaactgga
       61 tctgatatta ttttaaaaaa gattacaaaa ggtgccacgt cggaaaccat cataaaagca
      121 tgcaaaaaag caatggaaac tggatttacg ctatcatgca ttatcatttt gggtttggga
      181 ggaaaaactt acacaaagga acacattgag ggaacagctc gtgtagtaaa cgctgcatca
      241 ccacattatc ttggtacatt aactctgatc ttggaaacag gggttaagga agagttccta
      301 acaaagtttg gtgaagaatt ttatccaata aatgatgatg aagctttgat tgagctccat
      361 gatttagttc aacaaattga catcaaagaa aaaatggtgt ttagagcaaa tcatggttct
      421 aatgcataca acatagctgg aacattccct gatgagaaaa atgcgatgct tgaaaaaatt
      481 acttggttaa aggaacaccc agaaaatatt aggccagaag ggtttagagc tttctaatgg
      541 gtccacaggg atttgaaccc tggattttcg ccgtgtaagg gcgacgtcat aaccgagcta
      601 gaccatggac ccagcaaatt tcttgctaaa aaccatttaa actttgagaa gttatttttt
      661 ggaatcagaa cccgagcgta tattcccaaa aaccacaaaa tttcaaacta acgaataata
      721 atgttgaatt ttctatacta cattatgaat tcagtagtaa tcgcaaagtt tggcggaagc
      781 gtgattggga ttgatggagc ctcaatacca ttaatcattg agaggatcaa atcactgact
      841 ggcaaatcca aagtaattgc agttttttct gcccctttaa ctgcagttga tggaaaccgc
      901 agatcactga cagatgttgc gctagaactt ggaaaaatag ctgaggatgg aagtgtccct
      961 gatttaacta ttgtaagaaa aacatatgaa aaaatttccg agttggtgga ttctaaattt
     1021 caaaatgaat gtaagatgat gattgatgac tacttagaaa aagtgaagac agaattagaa
     1081 aaagtggtaa aaaataaaga gtttgcagat gaaacccgtt ctaaaacact ggcgttttct
     1141 ggggaaatca taatgtcgca aatgatggaa tatattctaa aaagtcataa catcaaatca
     1201 caatttgttg gactggaaga ttggccaatt ataactgata ataatataga atctacaaat
     1261 tttcttgtaa acgaatcaag caaaaatatt gaacatatag aaaaaatgct tgaaaaaaat
     1321 gatgtgatat caatcggtgg tttcataggt cgtaccaagg atggaataat aacaacatat
     1381 gaaagaggtg ggtcagatag aaccgctgca gatttgggaa ttttgttgca caaaaaattt
     1441 gatgtaaaaa ttgatttaga gaaagacaga tcagttgttt cagcagatcc taaaattgtg
     1501 agtgatggac tgacagaagt cagtagttta tcatacaatg aagccagatt agctggaatg
     1561 tttggaatga atattctaga tccgattgca attaaagaga ttttggaaaa tggtgcatca
     1621 ctgccaataa ttataacaga cataaaaaat cctgacaagt gcacagaagt tgcgaggaaa
     1681 atttctgccg gaaatggtca tccaataaaa attgttactg gaaaaaagaa ctgtgcaata
     1741 ttaaaaatag aggaggagac agtaggtgaa ttattaaaat cacttgaaaa tgaaaaaaga
     1801 tacagtgagt ttgtagaatt atctccattc atcaaagacg gaataaaatt tgctagaatg
     1861 ttattccttg atggtgatta tgtcaaacga aacgaaaaat atgttttggg ctttgatgcg
     1921 ctggcaacaa tcacatacaa tagaggcgtg ataacattga ttggggatga aatgtggaga
     1981 gttcaacaaa ttgtgtctaa aactagcgca aaaataggtg aggatggact caatatactc
     2041 aacatagatg cgcaagagga aacttcaaga ataattattg tgattgagga ttctgcagat
     2101 aacatcaaaa aagctattaa tgcagttcat agtgaacggt caaatatcaa attcgtttag
     2161 gcgtaattat ttaaattacc aaaattggat agagcaatat gaaagagaca ggtaaaattt
     2221 ggatgaatgg aaagttaatt ccattcaagg atgctaaggt tcatgttctt actcatgcgt
     2281 tacattactc aacagctgtt tttgaaggaa taagatgtta taatacacct gatggttctg
     2341 caatatttcg actgccggaa catgttgata ggttttttaa ttctgcaaaa atgtatggta
     2401 tgaaaatgcg ttactcaaaa aaacaaatca gtaacgcaat aatcaagaca gtaaaaac
//
LOCUS       DASCPJ010000021         2002 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000349941, whole genome shotgun sequence.
ACCESSION   DASCPJ010000021 DASCPJ010000000
VERSION     DASCPJ010000021.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000349941"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(247..1974)
                     /gene="ilvD"
                     /locus_tag="QGF01_01305"
     CDS             complement(247..1974)
                     /gene="ilvD"
                     /locus_tag="QGF01_01305"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HJN19570.1"
                     /translation="MEISSRNVVEGTARSPHRAMYKAMGLTDADIAKSFIGVCHTGNE
                     ATPCNIHLPGLAQKAKDGVSEAGATPREFSTIAVSDGIAMGHEGMKSSLVSREVIADS
                     IELMVRAHQYDALVGIAGCDKSLPGTMMAMARLNIPSVFVYGGTIMPGILDERELTVV
                     DVYEAVGQYDAGKISIEELKKIEDNACPNAGSCGGMFTANTMASISEMIGLALPGSAS
                     PPAENDRREKIVYDTGKACVNLLEIGIKPSDILTFEAFENAITMLNAVGGSTNGILHL
                     LALSNEAGIKLTYDDFERIRKKTPHIADMKPGGEYVMNSLDKIGGIPFIMKKLDDKSL
                     IHSNALTVTGKSIKENLAKIIIPEQSDQQVVKSVETPIHNVGTAVILKGSLAPDGAVI
                     KTAGVDMTKFIGTAKVFDTEEVAFDSISKGEIEEGDVIVIRYEGPKGGPGMREMLATT
                     AAIVGQGLGKKVAMVTDGRFSGGTRGFMVGHVAPEAFVGGPIAFIKNGDKISINTEDN
                     SIDLEVSSDELESRKKEWKMPKPKYESGALAKYAFLVGSAANGAITDPTNFLKMTKYL
                     QKNNLIRED"
BASE COUNT          610 a          420 c          289 g          683 t
ORIGIN      
        1 ggatttttga cactaaaaac cacataaatt tggagctaaa atttagtagt gaatcaaaga
       61 aattaagttt tgttcctata ctcaaaatta ctgtatagta cggtaccctt attagtaatg
      121 tggcataata gaatttatga agaaaaaatg tcacaagtgc ggcacaattg gcttgaattg
      181 gagtcaatca taccataggc aaacgggtaa atggaaactt gaaaaccata aaggctgtca
      241 ggatatttaa tcttcacgta tcaagttatt tttctgaaga tatttggtca ttttaaggaa
      301 gtttgtggga tcagttattg caccattagc agcagaacca acaagaaatg catatttggc
      361 taatgcacct gattcatact tgggttttgg catcttccat tccttctttc tactttcaag
      421 ttcatcgctt gaaacctcaa ggtcgatact attatcctca gtattaatac taattttatc
      481 accatttttg ataaaagcga ttggtccacc aacaaatgct tcaggtgcca catgaccaac
      541 cataaatcct cgggtccctc cagaaaatct accatcagtt accattgcaa ctttttttcc
      601 aagaccttga ccaactatag cagctgtggt tgcaagcatt tctctcatac ctggaccacc
      661 ttttggacct tcatatctaa taacaataac atcaccttct tcaatctcac cttttgatat
      721 tgaatcaaat gcaacttctt ccgtatcaaa aactttagcg gtcccaataa atttagtcat
      781 atcaacacct gcagttttaa ttactgcacc atcaggtgct aaactaccct tgagaattac
      841 tgctgttcca acattatgta tgggggtttc aactgattta acaacttgtt gatctgattg
      901 ttctggaatt attattttag caagattttc tttaattgat tttcctgtga cagttaatgc
      961 attggaatga ataagtgatt tgtcatcaag ttttttcata ataaatggga tgccaccaat
     1021 tttatccaaa ctattcatca catattcacc acctggtttc atatcagcaa tatgaggtgt
     1081 tttttttctg attctttcaa aatcatcata tgtgagtttt attccagctt cattagataa
     1141 tgctagtaaa tgtaaaatac catttgttga tcctcctact gcattcaaca tagtaattgc
     1201 gttttcaaat gcttcaaatg ttaaaatatc acttggcttt ataccgattt ccaataaatt
     1261 aacacatgct ttacctgtat catatactat tttttctctt ctatcatttt cagctggtgg
     1321 acttgcacta ccaggtaatg caagaccaat catctcagat attgatgcca ttgtattggc
     1381 tgtgaacatt ccaccacatg aaccagcatt agggcatgca ttatcttcta tcttttttaa
     1441 ttcctctatc gaaatttttc cagcatcata ttgacctacc gcttcataca catcaacaac
     1501 tgttaactct ctttcatcaa gtataccagg cattattgta ccgccataaa caaaaacaga
     1561 aggaatgttt aatcttgcca ttgccatcat agtaccaggt aaacttttgt cacatccagc
     1621 aattccaact aatgcgtcat attgatgagc tcttaccatt aattcaatag aatcagcgat
     1681 aacttctcta ctaactaatg atgatttcat accctcatgc cccattgcta ttccatcact
     1741 tacggcaatt gtactaaatt ctctaggagt tgcaccagct tcacttactc catctttagc
     1801 cttttgcgca agtcctggta agtgaatatt acatggggtt gcttcattac ctgtatgaca
     1861 aacaccaatg aaggattttg caatatcagc atcagttaag cccatagcct tgtacatagc
     1921 ccgatgaggt gatctagctg ttccttccac tacatttcta ctagatattt ccatttttaa
     1981 tacaataaag taaaattact at
//
LOCUS       DASCPJ010000022         5619 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000360901, whole genome shotgun sequence.
ACCESSION   DASCPJ010000022 DASCPJ010000000
VERSION     DASCPJ010000022.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000360901"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(149..1045)
                     /locus_tag="QGF01_01310"
     CDS             complement(149..1045)
                     /locus_tag="QGF01_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HJN19571.1"
                     /translation="MRFVVTGGAGFVGSHLVKLLLKNDHDVIVIDNLHTGKKENLGSV
                     IDKIEFQELDIRNHESIKNILKNIDGVFHQAALTVVQDSFSNPKEYHDVNVQGTENIF
                     KLSIENKFKVVYASSSSVYGHKQKIPIEESVERNPINPYGQTKLECEHLFEKYSKLDG
                     SIIGLRYFNIFGKGQTIEYAGVITKFIERLKEGKAPIIFGDGSQIRDFIYVNDIAKAN
                     LMAMNCNESNLLVNIGSGKAISVLELANMMIKISELELNPIFTEALQGDIEKSHADIE
                     LAKRSFGWKPEITLQEWLKEII"
     gene            1137..2513
                     /locus_tag="QGF01_01315"
     CDS             1137..2513
                     /locus_tag="QGF01_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HJN19572.1"
                     /translation="MIKNFSVLDDSQIQECLNNKSLKIGIVGIGRIGLPTALSFANSG
                     FETIGIDINKELVDMVNSGNYPLKDEPEFDKIFENVYNNKKITATTDISEAIPHCDVV
                     ILSLPTPMDDQNIPDYSALLTVGKNLNQLLSNGQIVIVESTVEPGFIENELLQSIEGH
                     DRTIKSGIDFHLAVCPETANPGEIMNDFKKLPRLVSAIDKRISSIVSQIYTHIFDVEL
                     ISMPNCKTANAVKLTTNVFRDVNIAFVNELAILFEKLGIDTYTVIEAAKRKYNFQAHY
                     PGPGVGGPCLPVNSYQYINSSKKIGADLLKIVQHARKINEKMPEHVIELTSDAFNECK
                     KPIQDSHILILGVSYKPNVKDVQLSPAEHIVRKFQNLGVNVHIYDPYFLSTEVFGIRV
                     EENIDDILSKIDAVVIVTNHDDFKKIEISSFNKMKNPILIDTRGIFDPASVKHHKIIF
                     RGLGRGDF"
     gene            2500..3702
                     /locus_tag="QGF01_01320"
     CDS             2500..3702
                     /locus_tag="QGF01_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HJN19573.1"
                     /translation="MEISSSSITDILTLRYDPSINPNLPKKTWKDFESINESPDIDFI
                     ENSILEEIENKLKSLSSKNVSIALSGGIDSTLVLSLLRKTNPDLKINAVSIKFANSVD
                     ETPVASKIAEKYDAEHHIIYLENFIAELPKAISITKLPFWDLHWYYVVKKSQTFSKIL
                     ISGDGGDELFSGYTFRYKKFLSLTSENSTPLEKVKAYLQCHERDRVTDQEKIFDEKCG
                     FSWNSIYNTLLPYFDNSLPRLEQVYLADYNGKLLYNFNPVNSRLTDYFEMKQLTPLLN
                     NKLLNTAPHIASKYKYDIDNEIGKLPLRTILAKNNAESLVTKEKLGFSVNTTNLWKSY
                     GHDLCKEFLGNSRVVQDGWINKDWILKHIDNSELDVKYINKFLGILAFEIWFRIFVTK
                     EMNGNESL"
     gene            3797..4279
                     /locus_tag="QGF01_01325"
     CDS             3797..4279
                     /locus_tag="QGF01_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19574.1"
                     /translation="MAKNRPFRAWFYFRQGWTAYFAFIMAAINVLTVTYFLAIENYPV
                     LQAIFPTFGHYIAIVVGTGVPLLVLVGYFHYKRSQAYAAEADINIEANPYWYKIPPGW
                     NKEVVFPLYLNMINLMLKMSKNEKLAPDEIEKMSDIQKSLSNLIDGGYVGKPFRMKDD
                     "
     gene            4272..5000
                     /locus_tag="QGF01_01330"
     CDS             4272..5000
                     /locus_tag="QGF01_01330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJN19575.1"
                     /translation="MTDFDYQWKNLLGKEALKDMEQMFECNNDRVKEFLGLTGIKSWY
                     KKDSFIKDKICLDAGCGPGRWTCAMQRLGAKKVDSFDLSEEAINRCKKINPNAYIFNI
                     WDLKPNPIYDFVLSWGVLHHTKKPREAFAKVASQIKKNGMLHVMIYNKENDWVYEGFR
                     GETCVEKHEYWITLSEQEKIKLCENMVKKHRGNIHGWFDALNPTYNWSYSSDEIESWF
                     KEEGFSEIKLRMVKQNINMNGILS"
     gene            complement(5047..>5619)
                     /locus_tag="QGF01_01335"
     CDS             complement(5047..>5619)
                     /locus_tag="QGF01_01335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJN19576.1"
                     /translation="PENWYHTDGMKLKHPCVGLLQSAVIWEKTKEMLTITKVLKKMPE
                     VNFYWAGDGPYRDKILPELEKFDNFHWLGNLEYPNKVRDFLTEIDIYALVSGLDMSPL
                     TLQEAQLMKKPVIATNVGGIPELMKNNKTGFLVKKGQGDDLHEKIEKLINDKDLQINF
                     GKTGREFIENNFTWDIIADNFLKNIKTKIN"
BASE COUNT         1962 a          842 c          893 g         1922 t
ORIGIN      
        1 agttacaccc tatttggtag gaggtgatac ttctacaact ttagttgatg gcactggatt
       61 ttctaagata ataggatcaa caaaattaaa actaaaaaat gttagaaaag taaaaaatga
      121 gattatgcta cattatcagt gtatttgatt atattatttc ctttaaccat tcttgaagtg
      181 taatttcagg tttccaacca aatgatcttt tagctaattc tatgtcagca tggcttttct
      241 ctatatcacc ttgtagtgct tcagtaaata ttggatttaa ttccaactca gatattttga
      301 tcatcatgtt tgctaattcc aaaactgaga ttgctttacc tgaaccaatg ttgactagta
      361 gatttgattc attacaattc atagccatca gatttgcctt tgcgatatca ttaacataaa
      421 taaaatctct aatttgtgaa ccatccccaa aaattattgg ggcttttcct tcctttaatc
      481 tctctataaa tttcgttata actcctgcat attctattgt ttgacccttg ccaaatatgt
      541 tgaaatatct taatccaatt attgatccat ccaactttga atatttttca aacagatgct
      601 cacattcaag ctttgtttgt ccatatgggt ttattgggtt tctttccaca ctttcttcta
      661 ttggtatttt ttgtttgtga ccataaacgc ttgagctact tgcatatact actttgaatt
      721 tattttctat tgagagtttg aaaatatttt ctgttccctg tacattcaca tcatgatatt
      781 ctttaggatt tgaaaatgaa tcttgtacaa ctgtaagtgc tgcttgatgg aacactccat
      841 ctatattttt caagatattt ttgatggatt catggtttct aatatctaat tcttgaaact
      901 caatcttatc tatcacagac ccaagatttt ctttttttcc tgtatggaga ttatcaatta
      961 ctattacatc atggtcattc ttgaggagaa gtttcaccaa atggcttcct acaaatcctg
     1021 cgccacctgt aaccacaaaa cgcatatggc aactaatatt tgtctgtctt taagtattag
     1081 gcataataaa caataaatcc aaaactacaa aaacaacaat ttacgcgatt aaatacatga
     1141 tcaaaaattt ttcagtgtta gatgattctc aaattcagga atgccttaac aataaatcat
     1201 tgaaaattgg aattgtaggt attggccgaa ttggactacc taccgcatta tcctttgcaa
     1261 attctgggtt tgagacaatt ggaattgata tcaataaaga attagtcgat atggtaaact
     1321 ctggaaacta tcccttaaaa gatgagcctg aatttgataa gatatttgag aacgtatata
     1381 ataataaaaa aattactgca acaactgata tttctgaagc aattcctcac tgtgatgttg
     1441 taatactatc tttgcctact ccaatggatg atcaaaatat acctgattat agtgcattgt
     1501 taactgtcgg aaaaaatctt aaccaattat tgtctaatgg acaaattgtt atagtagaga
     1561 gtactgttga accaggattt attgaaaatg aattacttca atctattgaa ggtcacgata
     1621 gaactattaa atctggaata gattttcatt tagctgtttg tcctgaaaca gctaatccag
     1681 gagaaataat gaatgatttt aagaaattac ccagacttgt tagtgcaatt gataaaagaa
     1741 tttcttcaat tgtatctcaa atttatacac atatttttga tgtagaacta atttcaatgc
     1801 ctaactgtaa aacagcaaat gccgtaaaat tgaccacaaa cgtgtttcgt gacgttaaca
     1861 tcgcatttgt aaatgaatta gcaatattat ttgagaaact gggtattgat acatacactg
     1921 taattgaagc tgcaaaacgt aagtataatt ttcaggctca ttatcctgga ccaggtgtag
     1981 gtggaccatg tttacctgta aattcatatc aatacataaa ctcatcaaaa aaaattggtg
     2041 cagatttatt aaaaattgta caacatgcta ggaagattaa tgaaaaaatg ccggaacacg
     2101 ttattgagct tacttctgat gcatttaatg aatgtaagaa accaatacag gatagccaca
     2161 ttctaattct tggcgtttca tacaaaccaa atgtaaaaga tgttcaatta tctccagctg
     2221 aacatatagt aagaaaattc caaaatttag gcgtgaatgt tcacatctat gatccctatt
     2281 ttctttcaac cgaggtattt ggtatcaggg ttgaagaaaa cattgatgac atattatcaa
     2341 aaattgatgc agtagtaatt gtaacaaacc atgatgattt taagaaaatt gaaatttctt
     2401 ctttcaataa gatgaaaaat ccaatattaa ttgatactag aggaattttt gatccagcat
     2461 ctgtaaagca tcataagata atttttagag gattaggtcg tggagatttc tagttcgtca
     2521 ataacggata ttcttactct tagatatgat ccttctataa atccaaattt acctaagaaa
     2581 acttggaaag attttgaatc tattaatgaa tctccagata ttgattttat tgagaattca
     2641 atattggaag aaattgaaaa taaattaaaa tctctaagtt cgaaaaatgt ctcaattgct
     2701 cttagtggag gaatagattc tacattggtt ttatccttac ttcgcaaaac aaatcctgat
     2761 ctaaaaatta atgcagtttc gataaaattc gcaaatagtg ttgatgaaac gccagttgca
     2821 tcaaagatag cagaaaaata tgatgctgag caccacataa tctatctaga gaattttata
     2881 gctgaattac caaaagcaat tagtattacc aaattaccat tttgggattt acattggtat
     2941 tatgtggtaa aaaaatcaca gaccttttct aaaatattaa tctctggtga tggtggtgat
     3001 gagcttttta gtggctacac gtttagatac aaaaaatttc tttctttaac gtctgaaaat
     3061 tcaactcctc ttgaaaaagt taaggcatat ttacaatgtc atgaaagaga tagagtaact
     3121 gatcaagaaa aaatttttga tgaaaaatgt ggattttcat ggaattctat ttacaacacg
     3181 cttttaccat attttgataa cagtcttcca cgattagaac aagtttatct agctgattat
     3241 aatggtaagc ttttgtataa ttttaatcca gtaaactctc ggctgactga ttattttgaa
     3301 atgaaacaat taaccccatt actaaataat aaattgttaa acacagctcc gcatattgct
     3361 tctaaataca aatatgatat agataatgaa ataggaaaat tacctttacg tactatttta
     3421 gcaaaaaata atgctgaatc attggtaact aaagaaaaac tgggatttag tgtgaataca
     3481 actaatctct ggaaatcata tgggcatgat ttatgtaaag aattcttagg caattcaaga
     3541 gtagtacaag atggatggat aaacaaggat tggattctga agcatattga taattcagaa
     3601 ttggatgtaa aatatatcaa taaattcctg ggtattttgg cgtttgaaat atggtttaga
     3661 atatttgtta ccaaagaaat gaatggtaat gaatcattat agccttaaaa attacctaaa
     3721 aacagaaaat tttcttttta gttagacaac ttttttgata gtattgttga tatataatca
     3781 atctaaaatt ttatgattgg ctaaaaatag accgtttcgt gcttggtttt atttcaggca
     3841 aggatggaca gcttactttg catttatcat ggctgcaatc aatgtgttaa cagttacata
     3901 ttttcttgca atagagaact atcctgtttt acaagcaata ttcccaacat ttggacatta
     3961 catagctatt gtggttggta caggagtgcc actattagtt ctggtcggct attttcatta
     4021 taaaagaagc caagcatatg cagcagaagc agatatcaat attgaggcta atccatattg
     4081 gtataaaatc ccaccaggtt ggaacaaaga ggttgttttt ccactatatc ttaacatgat
     4141 aaatctaatg ctaaagatgt caaaaaatga gaaattggct cctgatgaaa tagaaaaaat
     4201 gtcagatata cagaaatctc tgtcaaattt gattgatggt ggatacgtcg gaaaaccatt
     4261 taggatgaaa gatgactgat ttcgattatc aatggaaaaa tcttcttggg aaagaggctt
     4321 tgaaggatat ggaacaaatg ttcgaatgta ataatgatag agtaaaagaa tttcttgggt
     4381 taactggaat caaatcatgg tacaaaaaag attctttcat taaagacaaa atttgtttgg
     4441 atgcgggttg tggtccagga cgatggacat gtgcaatgca gagacttgga gcaaaaaaag
     4501 ttgatagttt tgatttaagc gaagaagcaa taaaccgttg taaaaaaata aatcctaacg
     4561 catacatttt taatatttgg gatttaaaac caaatccaat ttatgacttt gtcctatctt
     4621 ggggagtttt acatcataca aaaaaaccaa gagaagcatt cgcaaaggtg gcatcacaaa
     4681 tcaaaaaaaa tggaatgttg catgttatga tctacaacaa agaaaatgat tgggtatatg
     4741 aggggtttcg tggagaaact tgtgttgaaa aacacgagta ttggataaca ttatcagagc
     4801 aagaaaaaat aaaactttgt gagaatatgg taaaaaaaca tagagggaat atacatggtt
     4861 ggtttgatgc ccttaaccca acttataatt ggagttattc aagtgatgaa atagaatcat
     4921 ggttcaaaga agaaggtttt tccgaaatta aattaagaat ggtaaaacag aatataaaca
     4981 tgaatggtat tcttagttaa aatctatatt gcaaagaata tctattttca taatactggc
     5041 taaaatctag ttaatttttg tttttatatt tttcagaaaa ttatccgcta ttatatccca
     5101 agtaaaatta ttttcaatga actctctccc tgtttttcca aaattaattt gtaaatcttt
     5161 atcattgatc agtttttcaa ttttttcatg tagatcatct ccttgacctt ttttcacaag
     5221 aaatcctgtc ttattatttt tcattaactc tgggatacct cctacatttg tagcaattac
     5281 aggttttttc atcaactgtg cttcttgtag tgtaagtgga gacatatcaa gaccactaac
     5341 taaagcataa atgtcaattt cagttaagaa atctcgtact ttgtttggat attctaaatt
     5401 tcctaaccaa tggaaattat caaatttttc taattctggt agtattttat cacgataagg
     5461 tccatcacct gcccaataga aattaacttc tggcattttt tttaaaactt tagtaattgt
     5521 cagcatttct tttgtttttt cccagattac agcactctgt aataacccta cacatggatg
     5581 cttcagcttc attccatctg tatgatacca attttcggg
//
LOCUS       DASCPJ010000023         3687 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000370473, whole genome shotgun sequence.
ACCESSION   DASCPJ010000023 DASCPJ010000000
VERSION     DASCPJ010000023.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000370473"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(51..986)
                     /gene="nadA"
                     /locus_tag="QGF01_01340"
     CDS             complement(51..986)
                     /gene="nadA"
                     /locus_tag="QGF01_01340"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HJN19577.1"
                     /translation="MLKDEILKLKKEKDVVILAHNYQIPEVQDVADFVGDSLGLSRQA
                     AKVKEETILFCGVHFMAETAAIISPNKRVLIPDLKAGCSLSDSITIDQLKKWKNEHPD
                     AITVGYVNTTAEIKSELDYCCTSSNAVNVVNAIPKEKEILFLPDMFLGSYVSKMTGRK
                     NMQIWAGECHVHAGITPDDIERKLTEMKNAEFVIHPECSCTTPMMYDMASGHYKNHQV
                     QILSTEGMLNHVSKSDSQQFVVATETGILYRMRKQNPQKTFVPASENAECEYMKVITL
                     DKVYRSLYDDKYEVKVGKKIADKARLAIERMLSIS"
     gene            complement(1078..1896)
                     /locus_tag="QGF01_01345"
     CDS             complement(1078..1896)
                     /locus_tag="QGF01_01345"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HJN19578.1"
                     /translation="MKRIGLLGCGAIGTQIALAIDSGKIPAILTHVYDTKRSQADNLV
                     SKLQQKPEVVANSHLLSSNQLDLVVEAASQDAVRNDAVSILQNKKDLMIMSAGALLDE
                     SIFEVISDACKDFDKTVYLPSGAIAGLDALKAVKDELESVVLVTTKNPASLIGAPFFE
                     NSDINPDKISEPTILFEGVAKDAISLFPKNVNVAALLSLVGLGSKNTNVRVIADPNTN
                     KNTHQINAEGKFGKISVTVENIPDDTNPKTSRLATLSAIELLRQICSKGLEIGT"
     gene            2016..2174
                     /locus_tag="QGF01_01350"
     CDS             2016..2174
                     /locus_tag="QGF01_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19579.1"
                     /translation="MELEVDSDEKAEIAKIISSEDSPVGIDAKKTHIIIIHKLLEIEK
                     RLAKLEQK"
     gene            2271..3116
                     /locus_tag="QGF01_01355"
     CDS             2271..3116
                     /locus_tag="QGF01_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HJN19580.1"
                     /translation="MLNSVLKDAIKKRDETLSILKGPKFEQIVKQAKANWVDFSPTKQ
                     DVVAAGIDSSFNSTKFQGAELWAVTAVSVKSDGEILVDLHEHGLDSNTEIASLASKME
                     VDACIESVDETELVLMDGSIHSHFLKRQKPLTKSLVDAVTKKNNVVFISKTSNTKKQF
                     ENLGAIAGDIFYYNHATITPGFSKIHEDSRFEASGEAPIISSIFARLAESLPLIKIEL
                     LGSGYTNDDFKLILNKILKNSVGGYPYALKLAHNNCKISGRDLTKLASIHGLSNEIGS
                     RAILE"
     gene            3113..>3687
                     /locus_tag="QGF01_01360"
     CDS             3113..>3687
                     /locus_tag="QGF01_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HJN19581.1"
                     /translation="MTLGFVIGESKPTIVTAQTSRSLPIGEYVIINSNDGKIVGLVEK
                     SIVSSAALADVKNYDETVESIEVAVLNKRDKSHTAMIRILGFLESLQKGKAILPAIPP
                     TPGTEIIKATKDDLGQIFGPENKEWIKIGNLLRNPEINSLINLNKIVSRHLGILAMTG
                     MGKSNLVTLLAKQIAKLDGTVIIFDYHNDYSG"
BASE COUNT         1188 a          687 c          668 g         1144 t
ORIGIN      
        1 attggattag acctaagcct agatgttata aaataatatt gtattacttg ctagctaatt
       61 gaaagcatcc tttcaattgc caaacgagct ttatcagcta tcttttttcc taccttcacc
      121 tcatatttgt catcatataa tgagcgatag actttgtcta atgtaatgac tttcatatac
      181 tcacattccg cattttctga agcaggtacg aatgtctttt gaggattctg ttttctcatt
      241 ctatataata ttccagtttc tgttgcaaca acaaattgct gtgaatctga ttttgaaaca
      301 tggttaagca ttccttcagt tgagaggatt tgaacttgat ggtttttgta atggccagat
      361 gccatatcat acatcattgg tgtggtacaa ctacattctg gatggattac gaattcagca
      421 tttttcattt cagttaattt tctttcaatg tcatcaggag tgattccagc atgaacatgg
      481 cattcccctg cccatatctg catatttttt ctgccagtca tctttgatac ataagagccc
      541 aaaaacatgt caggcaaaaa tagtatttcc ttttccttag gaattgcgtt gacaacattt
      601 actgcgtttg aagatgtgca acaataatct aattctgatt ttatttcagc agtcgtgttc
      661 acataaccta cagtaattgc atcaggatgt tcatttttcc atttttttag ctggtctatt
      721 gtgatagaat ctgatagtga acaaccagcc tttagatcag gaataagcac ccttttgtta
      781 ggactaataa tcgccgcagt ttcagccata aaatgtacac cgcagaaaag aattgtttcc
      841 tccttgacct tagcagcctg ccttgataga ccaagcgaat ctcctacaaa atcagccaca
      901 tcttgaactt cagggatctg atagttatgt gcaagtataa ctacatcctt ttctttcttt
      961 aatttgagaa tctcatcctt taacataatc aaaaaattga accaatgtta caatttaaag
     1021 gataatgtta tgacataaat ttgtcttcaa aatcagtcat atgggcaaat tttgccacta
     1081 cgttcctatc tcaagccctt tactacaaat ttgtcttaaa agttctatgg cagaaagagt
     1141 ggctagccta cttgtttttg ggtttgtgtc atctggaata ttttccacag ttacggaaat
     1201 tttgccaaac ttcccttcag cattaatttg atgtgtgttt ttatttgtat taggatctgc
     1261 aataactcta acatttgtat tcttactccc taaacccact aaactaagaa gtgcagctac
     1321 gttaacattt ttaggaaata atgatattgc gtctttagcc acgccctcaa aaagaatcgt
     1381 aggttctgaa attttgtctg ggttaatgtc agaattttca aaaaatggtg cgccaataag
     1441 tgatgccgga ttttttgttg taacaagcac aactgattct agctcatctt ttaccgcctt
     1501 taatgcatca agaccagcta ttgcgcctga tggaagatat acagtcttgt caaagtcttt
     1561 acaggcatca gatatcactt caaatataga ttcatcaagt aaagcgcctg ctgacattat
     1621 catcaaatct tttttgttct gtaatatgct tacagcatca tttcgcacgg catcctgtga
     1681 tgcggcctcc actaccaaat caagttggtt tgaagaaagc aagtgagagt tggcaacaac
     1741 ttcaggcttt tgctgaagtt ttgaaactag attatcagcc tgtgatcttt tggtatcata
     1801 tacatgggta agtatggcag ggattttccc tgaatcaatt gcaagtgcta tctgcgttcc
     1861 tatcgcccca catcccaata atccaattct tttcaaatcg ataaaatgca attatgatta
     1921 taattaaacc atagccaata ggcaattatt tatcatgaaa tagtatagta ggtaattctt
     1981 acaatatttt aataccataa tcactaacga tagaaatgga acttgaagtt gatagtgatg
     2041 aaaaggctga aattgctaaa atcatcagca gtgaggatag cccagtagga atcgacgcga
     2101 aaaaaaccca cataatcatt attcataaac tgctggaaat tgagaagcgg ttagccaaac
     2161 tcgaacaaaa ataggatcta ttgattctaa gaggctatag tcccaattag atctcgagtc
     2221 agcaagtttt gcaccccaat attcatattc gataaaaccg atcctgctat atgttaaaca
     2281 gtgttctaaa ggatgcaatc aaaaaacgtg acgaaacgct gtcaattttg aagggtccaa
     2341 agtttgaaca gatcgtcaaa caggcaaaag caaattgggt ggatttttca cccacaaaac
     2401 aagacgtagt cgctgctggt atcgatagta gtttcaacag tacaaaattt cagggtgcgg
     2461 aactatgggc agttacagca gtttctgtca aatcagatgg tgaaattctg gttgatttac
     2521 acgagcatgg tcttgattca aacacagaga ttgcttcgct tgcaagtaag atggaggttg
     2581 acgcatgtat agaatcagtc gatgagactg aattggtact gatggacggt tcgattcatt
     2641 cccactttct aaaacgacaa aaaccgctaa caaagtcact agttgatgca gttacaaaaa
     2701 agaacaatgt tgtttttatc tcaaaaacat caaacactaa aaaacaattt gaaaacctag
     2761 gtgcgatagc aggggacatc ttctattaca atcatgcaac aattactcca ggattcagca
     2821 agattcacga ggactcccga tttgaggcaa gtggtgaagc accgattatc tcatcaatct
     2881 ttgccagatt ggcagaatca ttgccgttga tcaagattga acttttaggt tctgggtata
     2941 ccaatgatga ttttaagtta attttaaaca aaattctaaa aaacagcgtt ggtggatatc
     3001 cctacgcgct aaaacttgct cataacaact gcaagatttc aggcagggat ttgacaaaac
     3061 ttgccagcat acacggacta tcaaatgaaa ttggttcgag ggcgattcta gaatgacact
     3121 gggatttgtt attggtgaat caaagccaac catagtaacc gcacaaacat ctagatcact
     3181 tcctataggt gaatacgtta tcataaactc aaatgatggg aaaattgttg gtctggttga
     3241 gaaatcaatt gtttcaagtg cggcactagc tgatgtgaaa aattacgatg agactgtaga
     3301 aagcattgaa gttgcagtac tcaacaaacg agataagagt cacactgcaa tgattaggat
     3361 tctaggattt ttggaatcac tgcaaaaagg taaagcgata cttcctgcta taccaccaac
     3421 tccaggaact gaaattatca aagccacaaa agatgatctt ggtcaaatct ttggaccaga
     3481 aaataaagag tggataaaga ttggtaatct tctaagaaac ccagaaatca attcgttgat
     3541 taatctgaac aagattgttt caagacatct tggaattctt gcaatgacag gaatgggaaa
     3601 aagtaatttg gttacattac ttgctaaaca aattgcaaaa ctcgatggaa ctgtgatcat
     3661 ttttgattat cataatgact attctgg
//
LOCUS       DASCPJ010000024         3427 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000439697, whole genome shotgun sequence.
ACCESSION   DASCPJ010000024 DASCPJ010000000
VERSION     DASCPJ010000024.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000439697"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="QGF01_01365"
     CDS             <1..983
                     /locus_tag="QGF01_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJN19582.1"
                     /translation="NLNLDGVQFATQSDVCFGFLQLLAHAFILLFGSPQFKFCRLENA
                     MCGLVQAGSGSAHIGISVSEGGLKEISKNTLVVPYNDSHTLEQVLSKNKDVAGLIIEP
                     VLANMGLILPEKNFLSDVRKITKQHDVPLIFDEVVTGFRVSNGGAQKTFKIKPDITTL
                     GKALGNGFTIAAVGGKKEIMNKLAPGGNVYQASTFAGNPISVTAAINSIKTINKMENK
                     LYEKLARNCELLVDEIDDLATDYKIPHQINSLASMFQIFFTNKPVIDYKTSKKSNTKK
                     FHKLFSNLLKNGIFIAPSQYETAFLSDAHTDVDITKTVGAYGLSLEALRN"
     gene            980..1915
                     /gene="hemC"
                     /locus_tag="QGF01_01370"
     CDS             980..1915
                     /gene="hemC"
                     /locus_tag="QGF01_01370"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HJN19583.1"
                     /translation="MTFLIGTRGSKLSLAQTNWVISELQKQNLASEFQIKTITTKGDT
                     DTRSLFTIDQRGIFEKEIDKAVADKQVDFAVHSMKDVPSDIPDSLIIASIPKRESSND
                     VIITKDGSNLRTIQSKSIIGTSSLRRAVQVSRVRPDVVVKPVRGNIETRINKVMDGKF
                     DAIILAQAGISRLGSDVKHSALSTKDFPPSPGQGALAIICRSDDDKTISMLKKIEDKK
                     SRTEIEAEREISKYVESGCRFPVGALAIANSNMLKLTVTAYSVDGKKSITLEKTGDIE
                     NPKNLGQSLGEELKRSGIAEIASNWRESLEEWNKK"
     gene            1912..2658
                     /gene="cobA"
                     /locus_tag="QGF01_01375"
     CDS             1912..2658
                     /gene="cobA"
                     /locus_tag="QGF01_01375"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HJN19584.1"
                     /translation="MTGKVFLVGAGPGDPSLITLRAVELIKKADVVLYDRLVSKKILS
                     MIPKKAEAVYVGRAVGDDFKHQNDTNALMVKYAKIKRNIVRLKGGDPIIFGRGGEEAE
                     YLKKHKIQFEIVPGITSGIGSATYAGIPLTHRNFASSVVFVTGHEDFEKNKDAVRWKK
                     LAKSTDTIVIMMGLSRIGIICKQLSDGGIDKKTPVAVIQDGTTSKQRMITGTVSNITQ
                     KVKTSKIKPPSIIIIGKVVSLSKTIGWRKK"
     gene            2655..>3427
                     /locus_tag="QGF01_01380"
     CDS             2655..>3427
                     /locus_tag="QGF01_01380"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HJN19585.1"
                     /translation="MIKGKNIAITRSKDDSQEFIDLITREEGNPFPLPTIELVSKGEK
                     IVDEFLSALREDNYDYSVFMSSKAVSLLFDTAKKIGKFDELQLAVANTIVIAVGPKTK
                     IVLEKENIKVAYMPKRYSSVGIGEVFTKLNAVDKKVIVPRSGASTPFLKDLLEKIGLH
                     ITELYLYDVCAFRDTSQWNVFRKLFSQNKIDGIIFTSASSVRAFVEIMTKDYEQTELV
                     GALHKTSIIAIGPFTADELKKLNVENVIADVHTVSGAFDA"
BASE COUNT         1271 a          539 c          663 g          954 t
ORIGIN      
        1 ccaacttaaa tttggatggt gtacaattcg caacacaatc tgatgtgtgc tttggctttc
       61 tccaactcct tgcacatgcg tttatcctgc tcttcggatc tccccaattc aaattctgca
      121 gattggagaa tgcaatgtgt ggcttagttc aagctggttc tgggtctgca catattggaa
      181 tttcagtttc tgagggtgga ttaaaggaaa tatcaaaaaa cacactagta gttccctaca
      241 atgattcaca tacacttgaa caagttcttt caaaaaataa ggatgttgca ggtctgatca
      301 tagaaccagt tctcgcaaac atgggactga ttttgcctga aaagaatttt ctttctgatg
      361 taagaaaaat taccaaacaa catgatgttc ctctaatttt tgacgaagtt gtcacaggat
      421 ttagagtatc gaatggtggt gcacaaaaga ctttcaagat taaacctgat attactactc
      481 ttggaaaggc acttggaaat gggtttacaa tagctgcagt aggtggtaaa aaggagataa
      541 tgaataaact tgcaccaggt ggaaatgtat atcaagcaag tacatttgca ggaaatccaa
      601 tatctgtcac ggcagcaatt aactccatca agacaattaa caaaatggaa aataaattat
      661 atgaaaaact cgcaagaaat tgcgaacttt tggtagatga aattgatgat ctagcaacag
      721 attataaaat tccacaccag attaattcat tggcttcaat gttccaaatt ttctttacaa
      781 ataaaccagt gattgattat aaaacttcaa aaaaatcaaa cacaaaaaaa ttccacaaat
      841 tattttcaaa tcttctcaaa aatggaatat ttattgcacc atcacagtat gagactgcat
      901 ttttgtcaga cgcacatact gatgtagata ttacaaagac agttggtgcc tacggattat
      961 cattagaggc actgagaaat tgacattttt gataggaaca cgcggaagta agctatcgtt
     1021 ggctcaaaca aattgggtaa tatcagaatt acaaaagcaa aatctagcct cagaatttca
     1081 aatcaaaact ataacaacaa aaggcgatac cgatacacga tctctcttta caatagatca
     1141 aaggggaata tttgaaaagg aaattgataa agcagtagca gataagcaag ttgattttgc
     1201 tgtacatagc atgaaggatg ttccatcaga tatcccagat tcgttgataa ttgcttccat
     1261 accaaagcga gaatcatcta atgatgtcat aataacaaag gatggttcta atcttcgaac
     1321 aattcaatca aagtccataa ttggtacaag cagtttaaga agagcagttc aagtttctag
     1381 ggttagacca gatgtagtgg taaaaccagt aagaggaaat atagaaacaa ggattaacaa
     1441 agttatggat gggaaatttg atgcaatcat attagcacaa gcaggaattt ctagattagg
     1501 ttctgacgtc aaacactcag cattgtccac aaaagatttt cctccatcac ctggtcaagg
     1561 agccttggca attatctgta gatctgatga cgataaaaca atctctatgc tgaaaaaaat
     1621 tgaagacaaa aaatctagaa cagaaattga agcagaaaga gaaatatcaa aatatgttga
     1681 atcaggctgt agatttcctg taggtgcatt ggcaattgct aactcaaaca tgttgaagct
     1741 gacagttact gcatattcag ttgatggaaa aaaatcaatt acattagaaa agacaggaga
     1801 catagaaaac ccaaaaaatc taggacagag tcttggcgaa gaactcaaaa gaagtggtat
     1861 tgcagagatt gcttctaatt ggagagaaag cttggaagag tggaataaaa aatgactggt
     1921 aaagtatttc tcgttggcgc gggtcctggt gatccaagtc ttattacatt acgtgcagtt
     1981 gagctgatca aaaaggcaga tgttgtactt tacgacaggc ttgtcagtaa aaaaatactt
     2041 tcaatgattc caaaaaaggc agaagctgtt tacgttggaa gagcagtagg tgatgatttc
     2101 aagcatcaga atgatacaaa tgctctcatg gtaaaatatg caaaaataaa acgtaatatt
     2161 gttcgattaa agggaggtga cccaataata tttggtagag gaggagaaga agcagaatat
     2221 ctgaaaaaac ataagataca atttgagatt gttcccggta ttacatctgg aattggctct
     2281 gcaacatatg cgggaatacc attaacacac agaaattttg catcatccgt agtatttgta
     2341 accggtcatg aagattttga aaaaaataaa gatgcggtga gatggaaaaa acttgcaaaa
     2401 tctactgaca caatagtcat catgatgggt ctttctcgta tcggcatcat ttgtaaacaa
     2461 ttatcggatg gaggaattga caaaaaaact cccgtcgctg taattcagga tggaactaca
     2521 tcaaagcaaa gaatgattac gggtacagtt tcaaatatca cacaaaaggt taagacatca
     2581 aagataaaac caccatcaat aattataata gggaaggtgg ttagtctttc aaaaactatt
     2641 ggctggagaa aaaaatgatt aaagggaaaa atatagcaat tacacgttca aaggatgatt
     2701 cacaagaatt tattgattta attacaaggg aagaaggaaa tccatttccg cttccaacga
     2761 tagagttagt aagcaaggga gaaaaaatag tagatgaatt cctatctgca cttagagaag
     2821 ataattatga ttattcagta tttatgagtt caaaagcagt atcacttcta tttgacactg
     2881 caaaaaaaat tggaaaattt gatgaattac aattggcagt agcaaacacc attgtgattg
     2941 ctgtaggtcc taaaacaaaa atagttttag agaaagaaaa tatcaaagtt gcatatatgc
     3001 caaaacgata ttcatcagtt ggcataggtg aagtgtttac aaaattaaat gcagttgata
     3061 agaaagtaat tgtgccacgt agtggtgcat ctacaccttt cttgaaagat cttttagaaa
     3121 aaattggttt gcatataact gaattgtatc tgtacgatgt ttgtgcattt cgtgatacgt
     3181 cacagtggaa cgtattccgc aaactttttt cacaaaacaa aattgacggg attattttta
     3241 ccagcgcatc atcagttaga gcatttgttg aaatcatgac aaaggattat gaacagacag
     3301 aacttgttgg cgcgttacat aaaacatcaa tcattgctat aggaccattt actgcagatg
     3361 aattaaaaaa acttaatgta gaaaatgtaa ttgcagatgt tcatactgtt tcaggcgctt
     3421 ttgatgc
//
LOCUS       DASCPJ010000025        16063 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000444265, whole genome shotgun sequence.
ACCESSION   DASCPJ010000025 DASCPJ010000000
VERSION     DASCPJ010000025.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 16063)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16063)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16063
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000444265"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /locus_tag="QGF01_01385"
     CDS             <1..2238
                     /locus_tag="QGF01_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HJN19586.1"
                     /translation="SASTGDVIEIKGKRRTVAKCLPLYPSDEGKGIIRIDGLGRNNSG
                     IAIGDAITVTKIKAVAAEKIVVAPLEAIPPIDERYLADALESVPLIKGDNVMVPYFGG
                     RLTFQVIGVTPNSDAVLVTQKTVFHIAEKGDTLRGVPQVSYEDIGGLRDEIKKVREMI
                     ELPLRHPEIFEKLGIEAPKGVLLYGPPGTGKTLLAKAVANESNAHFISISGPEIMSKF
                     YGESEARLREIFKEAREKAPSIVFVDEIDSIAPKREEVTGEVERRVVSQMLSLMDGLE
                     ARGKVIVISATNRPNAIDPALRRPGRFDREIEIKVPDKKGRADILNIHTRNMPLGDDV
                     DIKRIAGVSHGYVGADLEYLCKEAAMKCLRRLLPEINLDDEKIPPETLDKLIVNGEDY
                     KKALIEVTPSGMREVYIENPDVQWNEVGGLNDTKQELQEAVEWPMKYPGLYEKLGHKM
                     PRGILLHGPSGTGKTLLAKAVATESEANFVSVRGPELLSKWVGESERGIREIFKRARQ
                     SSPCVIFFDEIDSIAPIRGGGAETQVTERVVSQLLTELDGMQDMHGVVVLAATNRADM
                     IDPALLRPGRFDKIIQIPLPDKESRQRILEINAEEIPFEKDPKSEDYVNFSKLAEASD
                     GFSGADVAAIANTAVSFVIHEHLDKFAMSGQAKTKVTETIVTEKKIPPKNVDLIVEEK
                     EVRSISSAEEKAMKDKEEKEITIVEKAAENAKVTMKHFEDAIKKVREQKDLKIGQKVE
                     LSAFR"
     gene            2270..3556
                     /gene="gatD"
                     /locus_tag="QGF01_01390"
     CDS             2270..3556
                     /gene="gatD"
                     /locus_tag="QGF01_01390"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HJN19587.1"
                     /translation="MSEDQGYSGNALDCLKKYNIKVGDSIKITADLTYSGILMPRYES
                     GDDKHLVIKLGSGYNVGIDVDDIDKIQIVSSSEIKPKQGEERKDDPTLPKILLLSTGG
                     TIASKVDYRTGAVTPALSASDLNEAVPELGKIANIDTEVLFSEYSENLQPEHWTEIAE
                     KLDSLANSDYKGILIAHGTDTMHYTSSFLSFALAGFPIPVVLVGSQRSSDRPSSDAAI
                     NLIAASKFLIESNTKGIFLVMHHNDNDQTVACHAGTRVRKNHTSKRGAFETMGGDPAF
                     IVTPTLEIQKNIQEEFFKDAKYIPRMKIDTKVALVKYHPGYDPKLIENIIQTGCKAII
                     FEGTGLGHVGRTMYNVVKNAKENDLFLGMTSQCIDGRVRMTVYESGRDLLELGITPLE
                     NMTPETSLVKAMWALGNSKDMDEMKSLMLENIASEF"
     gene            3559..5454
                     /gene="gatE"
                     /locus_tag="QGF01_01395"
     CDS             3559..5454
                     /gene="gatE"
                     /locus_tag="QGF01_01395"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HJN19588.1"
                     /translation="MAEIDFEKIGLKVGLEIHQQLNTNKKLFCKCKPIESDEYTERFS
                     RRLRTAKSELGELDPAALFEKTKSKKINYYANSQSSCLVEKDEEPPHDLDHNAKKISL
                     LISSMLESKIFSEIHVMRKTVIDGSNTTGFQRTMLVSQGGNLKVNGKKIGVQAVCLEE
                     DAAKLLKDEQNERDYSLDRLGVPLVEIALEPVSTKPSEVKEIALTLGRLLRATRMVKR
                     GIGSIRQDVNISVMNSGVVEVKGVQQLDQLEKIIGYEAKRQYGLIQIGDKLKKLSITI
                     TKEDVHDITEIFKDCESKIIQKALKSKAKIKVIRIRNFSGMFGFEPYSGIRLGKEIGQ
                     IVRFFGIGGVFHSDELPNYGINSADIDKVRKYLELVDEDGFLIIAGEDPKLDYAIDSI
                     IKRIQDATNGVPAETRAATQDGETIFLRPRPGASRMYPETDIPSISVIPEEIKLAMEN
                     IPKSWDESIAEIQKKYTLNSQLSEQIFDSEHMELFEKICENKKNSPNFVASILCSSIT
                     NLERQGLDATLLKPEHVIESFELLASGKIPKESLEIIFESIMSGKSENVSTAMQSTNV
                     SSMDEDKLNGILDEIIQNNMELVKKLGDKAITTLMGLAMKEVRGQASGKIVNELLEEK
                     IKNIIKK"
     gene            complement(5487..5747)
                     /locus_tag="QGF01_01400"
     CDS             complement(5487..5747)
                     /locus_tag="QGF01_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19589.1"
                     /translation="MAKVTRVSGRGTGSRKTDSKKSSSFSGKAFKEHQEKRDKATAGK
                     YITTGRGTGRRLVGSKKSTTSKSTKGATRVTGRGTGTRKSRG"
     gene            complement(5802..5886)
                     /locus_tag="QGF01_01405"
     tRNA            complement(5802..5886)
                     /locus_tag="QGF01_01405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5848..5850),aa:Leu,seq:cag)
     gene            5965..6129
                     /locus_tag="QGF01_01410"
     CDS             5965..6129
                     /locus_tag="QGF01_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19590.1"
                     /translation="MEFREIYCYNCHKTLGKYNVKFYTETQIAEIIQTIHADHIKIGH
                     HVEIRKKKSK"
     gene            complement(6131..7234)
                     /locus_tag="QGF01_01415"
     CDS             complement(6131..7234)
                     /locus_tag="QGF01_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HJN19591.1"
                     /translation="MSAKRDYYEVVGVSKNASINEIKSQYRKLALKFHPDRNKSPEAG
                     EHFKEISEAYAVLSDPKKRSLYDQHGHAGVDGRYSTEDIFRGAGANFDDVFSDLFGRQ
                     GRSSGGFDSIFQNLFGGGGGFGGFGNQRGRDMLYETSLTMEDVLHGKRMEIDLQKNVD
                     CPDCNGSGCSPGTSKTSCAECKGHGQVRVSRNMGGMSFVTVQPCRKCGGEGSIIEKPC
                     RKCKQGKVKGTKHLSFDLPTGIDDGDYVLSGEGESIPNGANGDLIIRVRIQTHPEFKR
                     DGRDIFYDAHITMIDASLGKSFEVPTLDGSHKINVDSGSQPNSIIKLKGKGLPGKGFH
                     GRGDQYVRLVVNIPKKLNKQQKNLLKEFDDSTN"
     gene            complement(7309..9210)
                     /gene="dnaK"
                     /locus_tag="QGF01_01420"
     CDS             complement(7309..9210)
                     /gene="dnaK"
                     /locus_tag="QGF01_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HJN19592.1"
                     /translation="MSEKIIGIDLGTSNSAAAVIQGGKPTIIPAAEGQTAHGKAFPSV
                     VAFPKDGSPMIVGSPAVNQAVTNPENTITKAKRKMGEDHIYKIQGKEYKPQQISAFIL
                     QKIKKDAEAFTGDKITKAVITVPAYFDDNQRQATKDAGTIAGLDVVRIINEPTAAALA
                     FGLDKAKQDMKILVFDFGGGTLDVTIMEMGGGVFEVMSTSGDTHLGGTDMDLVIMDYV
                     KEEFRKKEGIDLSSDKEAMARLQEACEKAKIELSGVMETEINLPYFHEKTTLQVKLTR
                     AKIESLVTSLVEKCKSSIDKAIEDAKISTSDITKIVLVGGPTRMPIVKKFVENAIGKN
                     PESGVDPMEAVAFGAAIQAGIMAGDVESDIVLLDVTPLTLGIETLGGVREPIIERNTT
                     IPTSKDKTFTTAADNQTAVTINVVQGERPMVADNVSLGSFNLTEILPAPRGVPQINVK
                     FDIDANGIINVTAKDLGSGKDAKITIESSTKLSDEQVEKMKKDAEEHAEEDKKKRESV
                     DIKNEAESYIYTTEKLINQDLKDKISQEQGIKATDAMKELKESLTKDQDEIKTKLDAL
                     KAIVNEITTEIYKNAAPPPGADQQKDDGAKSEENTKSEENTKSEENTKSEENTKSEPE
                     SKPTEEQSK"
     gene            complement(9221..9799)
                     /locus_tag="QGF01_01425"
     CDS             complement(9221..9799)
                     /locus_tag="QGF01_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HJN19593.1"
                     /translation="MSHINENTNDVSQADAENDNKNLEHYDESEIETTESEQTLHKLQ
                     IDELKERVDQTTEKWKRALADYDNLEKRTQQEIIDKVSSETGKLILEFLTVYENFIRA
                     KEVYAKDGVDITGLEGIIKNMNSLFSEYDVRPIDAMGKIFDPNLHEAISIVEDPKLAE
                     DTIIEEIAKGYIIRGKVLKYSKVCVSKKIIKK"
     gene            complement(9826..10959)
                     /locus_tag="QGF01_01430"
     CDS             complement(9826..10959)
                     /locus_tag="QGF01_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19594.1"
                     /translation="MKKEKIVVYGLTTEGYFLASQMAMKGADVSIIDESSPSAVLLKA
                     ETAKTYPNLTTFQEDEPLLSVEPIDVAISNAKYLFFAPRIRKTGQDLRTEITSKYKDA
                     TKALKKGSSIIYCLPTGLGGNQENISLLTHITGLDVGKTISYFYFPLNNLDETPEVIG
                     SLDNQDDKILSSLLTIEKKEKKFVSITSAECIYGINIIQYFTNRSAILEICKFVKDTK
                     STTFNNFKTVYLDDIIKGLYDLRLLGSSFEGANNLLYLTNASIKAIHSYIKRLIDTIR
                     TTLKKNELKASRTKIVLSWTLDKNEMRGDKIEILNSITSKLRDYIGDVDTSEGLVNSF
                     HSDKKTIIIACSMNDYTKIVSDKRSADLIIIKANPLFEIENKK"
     gene            11022..12047
                     /locus_tag="QGF01_01435"
     CDS             11022..12047
                     /locus_tag="QGF01_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HJN19595.1"
                     /translation="MKIWLDILTPKQILFFEPMIQRLKKNHSVLCTSRKYNQVTDLAK
                     IRKQKLVIIGKHGGGEKHDKLNASLSRSKLLVKKISKFSPDITISFCSPEAARISYGL
                     GIKHICFSDSPHAENVMRLVVPLVQKLLIPWIIPKKKFTKFGIDQNDIIQYKAIDAAT
                     ISKRKIKNIKKTVGKRIILVRLEEDEAAYSLKKRPIIPIIKEILKEFSGQEIVIMTRY
                     ASQKKYLQQIFQKKIKILSKVVDSKLLLYNADIFIGSGGTMTAESALLGIPTISYDAV
                     PNIIEDYLVRKKLVMREVNPKKMVSVMKKVFKSSNSRRKNRAKKMMSSMEDPYPILLK
                     TMKSILK"
     gene            12080..12151
                     /locus_tag="QGF01_01440"
     tRNA            12080..12151
                     /locus_tag="QGF01_01440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12113..12115,aa:Gln,seq:ttg)
     gene            complement(12160..12921)
                     /locus_tag="QGF01_01445"
     CDS             complement(12160..12921)
                     /locus_tag="QGF01_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJN19596.1"
                     /translation="MHPIIRLVNRNITISINPGFLIWQVIFPLIWIFVAGFAYTALID
                     EVSFGNKDLSYPAFLASGMIGFNIMNSTLVSGIIIWNDRRHGMFEQIMSGPFTRANYI
                     LSNITTIAIVGLVSAALITVVGYPVFFQSAEFSLITIPLIIFSSIVGAILFGSIASII
                     SARLRSSEGFNVIINTVFIFFAFVSTAFYPAAGAPQPLATIFYLNPLTYLVDIIRAGV
                     FGTITEFVILEMVVLITLASILFIIATKLLTKMDF"
     gene            complement(12905..13849)
                     /locus_tag="QGF01_01450"
     CDS             complement(12905..13849)
                     /locus_tag="QGF01_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJN19597.1"
                     /translation="MKSILARSLFKSYDGINAVDGIDLDVDEGQIFGFLGPNGAGKST
                     VIKMLTTLIQPTGGEITVLKINAMKEPLKIRKKIGVVLQQPSYEPTLSVEKSLDKYGM
                     MWNIDKKTRKARTEELLTAFNLVEIRKKRNEDLSIGQRRRVQVAREFMHDMDLLFLDE
                     PTVGLDPTARRQLLDFLKNKVKEKNLTIFYTTHVLTEAEYLCDNIAIINKGKIITVDS
                     PNELKNRFGKEKTIKIHISSNTETLPDLLENIQDCKIDYGTGATITIRSTNSEVVLMN
                     ILKILNQNNIAIDDLSAIPTNLEEIFLKVVSDSNAPDN"
     gene            13918..15333
                     /locus_tag="QGF01_01455"
     CDS             13918..15333
                     /locus_tag="QGF01_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HJN19598.1"
                     /translation="MSEFEEIIENILKQKPELTRENLEGLIDQKTQKIGAGYLTDQGA
                     LFLIASDLGISLTQSTKLEVGLKDLYVGAKEISLEGRVLNISPTKEFSRKDGSSFLLR
                     TITVYDNDSTASVKLWDDKANLPGIENLKPGDLVKIIKAYVKSDLNGSPTINIGSGSD
                     IEPINQESKIRSIDALAVDSSEIKQDQNNLVISGKIDGSVSTLEFTNKRGDPAKALKM
                     SLKGNNGASTKVILWGKDETSLPKVVSQDSKVLLLGVRTKMGNQGLEIHGNDGTMVKV
                     EGSKEIEPIILRIVAKTKNEQGKTIALGINDKKNMVYVSDSVNMLEPTNIGDVVECMP
                     SQVYGNSLIIDSGSLVRNIEDDGTLSTLSDLRTKISEIKSGNEYCVEAIILKEPERRD
                     VQTKAGETISLGEMFVEDTTGQIWIKGWRNQAKLLDGLSRGEVISVTSVNAKAGLEGR
                     TELFLTPSSTVNKKFNHPKNP"
     gene            complement(15311..>16063)
                     /locus_tag="QGF01_01460"
     CDS             complement(15311..>16063)
                     /locus_tag="QGF01_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr helicase"
                     /protein_id="HJN19599.1"
                     /translation="THNINWRTWCVAKKFGMVERGSIYDRKTGHFIYEQYQTTPLVKE
                     ALRELFHDKFDLLGTDKLLTRIKNNEIQIEWIDVVKFSKLAEPLLDHTTKYYSSPANV
                     DKAILDEVKKRLLKTKHRLICARCGKWQLAIITGEFEKRPKKLICKYCKGRQITATYY
                     SDYDLVKIIQKNHKSKKLSLEENHKFKRAWKVASLIETFGNNAITVLSGYGVGADTAA
                     RILRNMVDEEYMYKQIYEAERQYVMTRGFWDD"
BASE COUNT         5341 a         2634 c         2851 g         5237 t
ORIGIN      
        1 agcgcgtcga caggcgatgt tattgaaatt aagggtaaaa gaagaaccgt tgcaaaatgt
       61 ttaccgcttt atccatcaga tgaaggaaag ggtattatta gaatagatgg acttggtaga
      121 aataattctg gaattgctat tggagatgct attaccgtaa caaagatcaa agcggtggct
      181 gcagaaaaaa ttgttgtagc accactagaa gctataccac caattgatga aagatatctt
      241 gcggatgctt tagaaagtgt gcctttgatt aagggtgata atgtgatggt accatatttt
      301 ggtggtcgtt taacatttca agtcatcggc gtaacaccaa atagtgatgc agtacttgta
      361 actcaaaaaa cagttttcca tatagcagaa aaaggtgata cgttaagagg tgttccacaa
      421 gtttcctatg aagatattgg tggtttgcgt gatgaaatta aaaaagtaag agagatgatt
      481 gagcttccgt tacgtcatcc ggagatattt gaaaaacttg gaatagaagc tccaaaaggt
      541 gtgttacttt atggacctcc tggtacagga aaaacattgt tagcaaaggc agttgcaaat
      601 gaaagtaatg cgcacttcat tagcatttct ggccctgaaa ttatgagtaa attctatggt
      661 gaaagtgaag ctagattacg agaaatattc aaagaagcaa gagaaaaagc accatcaatt
      721 gtttttgttg atgaaattga ttcaatagca cctaaacgag aagaggtgac aggtgaagta
      781 gaacgaagag ttgtatcaca aatgctatca cttatggatg gattagaagc gagaggtaaa
      841 gtaatagtaa tttctgcaac aaacagacct aatgcaattg atcctgcatt aagaaggcct
      901 ggaagatttg atagagaaat tgaaattaaa gttcctgata aaaagggaag agctgacatt
      961 ctcaatattc atacacgaaa tatgccatta ggtgatgatg tagacattaa acgtatagct
     1021 ggagtgagtc atggatatgt tggtgctgac ctggagtatt tgtgtaagga agcagctatg
     1081 aaatgtttac gcagattgtt gccagagatt aacttagatg atgaaaaaat tccgcctgaa
     1141 acacttgata aactaattgt aaatggagaa gattacaaaa aagcattgat tgaagttaca
     1201 ccatctggaa tgagggaagt gtatattgaa aacccagatg tacaatggaa tgaagtgggt
     1261 ggtctaaatg atactaagca agaacttcaa gaggcagttg aatggccaat gaaatatcct
     1321 ggactctatg aaaagttggg acataaaatg ccacgtggta ttttgcttca tggtccaagc
     1381 ggtacaggaa agacattgtt agctaaagca gttgctactg aaagtgaggc aaactttgtt
     1441 tccgtacgtg gtcctgaatt gttatcaaag tgggtaggag aatctgagag aggaatcaga
     1501 gaaattttca aaagagcaag acaatcatca ccatgtgtaa tattctttga tgaaattgat
     1561 tcaatagcac caattagagg aggcggagca gaaacacagg ttactgaaag agttgtcagt
     1621 caattattaa ccgaattaga cggtatgcag gatatgcatg gtgttgtagt gcttgctgca
     1681 acaaatagag cagatatgat tgatccagct ttacttagac ctggtagatt tgataaaatt
     1741 atacaaattc cattaccaga taaggaatct aggcaaagaa ttttggagat caatgcagaa
     1801 gaaatcccat tcgaaaaaga ccctaagagt gaagattatg tgaactttag taaattagcg
     1861 gaagcatctg atggatttag tggtgcagat gttgcagcca tagctaatac tgctgtatct
     1921 tttgttattc atgaacacct tgacaaattt gctatgagtg gacaagccaa aacaaaagtt
     1981 actgaaacaa tagttacaga aaagaagata ccacctaaga atgtagattt gatagttgag
     2041 gaaaaagaag tacgttctat ttcaagcgca gaggaaaaag ccatgaaaga caaagaagaa
     2101 aaagaaatta ctattgttga aaaagccgct gaaaatgcca aagttacaat gaagcatttt
     2161 gaggatgcaa tcaagaaggt aagagaacaa aaagacttga agataggaca aaaagtcgaa
     2221 ctttcagctt tcaggtagtt ttaataaaat aactgcttaa accacctcaa tgtcagaaga
     2281 ccaaggatat tctggaaacg ctcttgattg tttgaaaaaa tataacatca aagtaggcga
     2341 ttctatcaag attacagcgg atttaacata ttcgggaatt ttaatgccta ggtacgaatc
     2401 aggtgatgat aaacatctag taatcaaact aggaagtggt tataatgtag gcatcgatgt
     2461 tgatgacatt gataaaatac agattgtttc tagttctgaa ataaaaccga agcagggtga
     2521 agaaagaaag gatgatccaa cacttcccaa aattttgctt ctttctactg gaggaactat
     2581 tgcaagcaaa gtagactaca gaactggggc tgttacaccc gcccttagtg cttctgatct
     2641 taatgaagca gtaccagaac ttggaaaaat tgctaatatt gatacagaag ttttattctc
     2701 tgaatattca gaaaatttac agccagaaca ctggacagaa attgcagaaa aactagattc
     2761 gcttgcaaac tcggactata agggaatact aattgcacat ggaacagata ctatgcatta
     2821 cacatcttct tttttgtctt ttgcacttgc tgggtttcct attcccgttg tacttgttgg
     2881 ttcacagaga tcgtctgaca gaccatcatc tgatgcagct ataaacctga ttgcagcatc
     2941 aaagtttctt attgaatcta atacaaaggg catttttctt gttatgcatc ataatgataa
     3001 tgatcaaact gttgcatgtc acgcaggaac tagagttaga aaaaatcata caagtaagcg
     3061 tggagcattt gagacaatgg gtggtgatcc ggcattcata gttacaccaa cattggaaat
     3121 tcagaaaaat atacaagagg aattttttaa agatgcaaaa tacatcccaa ggatgaagat
     3181 tgacacaaaa gttgctcttg taaaatatca cccaggttac gacccaaagt taattgaaaa
     3241 cattatacaa acagggtgta aggcaataat ttttgaagga acaggactag gacatgttgg
     3301 acgaacaatg tacaatgttg taaaaaatgc aaaggaaaat gacctgtttt tgggaatgac
     3361 atcacaatgt atcgatggta gggttaggat gacagtctat gaaagtggta gggatttgtt
     3421 agaattagga attactcctc ttgaaaatat gactccagaa acttcacttg taaaagcaat
     3481 gtgggcctta ggaaattcaa aggatatgga tgaaatgaaa tcactaatgc tagaaaatat
     3541 tgcttcagag ttttaattat ggcggaaatt gattttgaaa aaattggttt gaaggtggga
     3601 ttagaaatcc atcagcagtt aaatacgaat aaaaaattat tttgcaaatg taaaccaata
     3661 gagagtgatg aatatactga gagattctct agaagactca gaacggcaaa aagtgagctt
     3721 ggtgaattag atccagctgc attatttgag aaaacaaaat caaagaaaat caactattat
     3781 gcaaattcac aaagtagttg cctagttgaa aaagatgaag aacctcctca tgatctagat
     3841 cataacgcaa agaagatttc attactgatt agcagtatgc ttgaatcaaa aatttttagt
     3901 gagattcatg ttatgagaaa aactgttatt gatggatcca acacaactgg ttttcaaagg
     3961 accatgcttg tttcccaagg aggtaatcta aaggtaaatg gaaaaaagat tggagttcag
     4021 gcggtttgtc ttgaggagga tgcagcgaaa cttctcaagg atgaacaaaa tgaaagagat
     4081 tacagtttag atcgtttggg tgtaccatta gttgaaattg ctttagaacc tgtaagcact
     4141 aaaccttctg aagttaaaga aattgcatta acacttggaa gattgttaag agcaacaaga
     4201 atggtaaaaa gaggaattgg ttccattaga caggatgtaa atatttcagt tatgaatagt
     4261 ggagttgtgg aagtaaaggg tgtacaacaa ctagatcagc ttgaaaaaat aattgggtat
     4321 gaagccaaaa gacagtatgg attaattcag attggtgata aactcaaaaa actatcaata
     4381 acaattacaa aagaagatgt tcatgacatt actgagattt tcaaagattg tgaatcaaaa
     4441 ataattcaaa aggcattaaa atcaaaagca aaaattaagg taatacgaat aagaaatttt
     4501 tcaggaatgt ttggttttga accatactcc ggaattagat taggaaaaga aattggacag
     4561 attgtaagat tttttggtat tggaggagtt ttccattcag atgagctacc taattacgga
     4621 attaatagtg ctgacattga taaggtaaga aaatatcttg aattggtaga tgaggatggt
     4681 tttctaatta tagctggtga agatccaaag ttagattatg ctatagattc tataataaag
     4741 agaattcaag atgcaacaaa cggagttcca gctgagacta gagctgcaac acaagatggt
     4801 gaaactattt ttcttagacc acgtccaggt gcatcaagaa tgtatccaga aaccgacatc
     4861 ccttccatta gtgtaatacc tgaagaaata aaacttgcaa tggaaaatat tccgaaatca
     4921 tgggatgagt caatagctga aattcagaaa aagtacactc taaattctca gctgtctgaa
     4981 cagatctttg actcagaaca catggaacta ttcgaaaaaa tctgtgaaaa taaaaaaaac
     5041 tcgccaaact ttgtagcctc tatactctgt tctagcataa caaacctgga gagacaggga
     5101 cttgacgcaa cgctattgaa accagaacat gtcatagaat catttgagct gttagcgtct
     5161 ggcaaaatac ctaaggaatc actagaaatt atttttgaaa gcataatgtc cggtaaatca
     5221 gaaaatgttt caactgcaat gcaaagcaca aacgtttcaa gtatggatga ggataaattg
     5281 aatggaattt tagatgaaat aattcaaaac aacatggaac ttgttaagaa gttaggagat
     5341 aaagcaataa ccaccttgat gggacttgcg atgaaagaag taaggggtca agcttccgga
     5401 aaaatagtaa atgaattact agaagaaaaa ataaaaaata taataaagaa atgaaaaaat
     5461 gatactggtt ttagttaaat gttaatttaa cctctgctct ttcttgtacc tgttcctctg
     5521 ccagtaactc ttgttgcacc tttagttgat ttacttgttg tacttttttt gctaccaaca
     5581 agtcttctac ctgttcctct gccagtagta atatatttgc cagcagtggc tttatcgcgt
     5641 ttttcttggt gttctttgaa tgcttttcca gagaaacttg aagatttttt agagtcagtt
     5701 tttctacttc ctgtgcctct tccgcttact cttgtgactt ttgccatgaa taattaaaaa
     5761 catcttgagc ttttaaggtt ttcatcagtt tgattataat atgcgggaag tgggatttga
     5821 acccacgaac tcctaagaga taaggatctg aaccttacgc cgttgaccag gctgggcgac
     5881 tcccgcataa aagaaaggaa aaacccagaa taagttcctt tttaggatca aaacacaaaa
     5941 aaaacaaatc aaattttgta gattatggaa tttcgggaga tttattgtta taattgccat
     6001 aaaacccttg gtaaatataa tgtaaaattt tacactgaaa ctcagattgc tgaaataata
     6061 caaacaatcc atgcagatca tatcaaaatt gggcatcatg ttgaaattcg taagaaaaaa
     6121 tctaaataga ttaatttgta gaatcgtcaa attcctttag tagatttttt tgttgtttgt
     6181 tcagtttttt tggaatattc actactaatc ttacatactg atcacctcta ccatgaaatc
     6241 cttttcctgg taaacccttt cccttgagct taataatcga atttggttgg ctgcctgaat
     6301 ccacattgat tttatgggaa ccatctagtg taggaacttc aaaacttttt cctagacttg
     6361 catctatcat cgttatatgt gcatcataaa aaatatctct tccgtcacgt ttgaattcag
     6421 gatgtgtttg tatacgtact cggattatca aatcgccatt tgcaccattt ggtatagatt
     6481 caccctcacc agataataca taatctccat catctatacc agttggaaga tcaaatgata
     6541 gatgtttagt gcctttcact tttccttgtt tacattttct acagggtttt tctattattg
     6601 atccctcccc accacatttt ctacatggtt gaacagttac aaaagacatg ccgcccatat
     6661 ttctactaac tctaacttgt ccatgacctt tacattctgc acatgaagtt tttgatgtac
     6721 cgggtgaaca accagaacca ttacaatctg ggcaatctac atttttttgt aaatcaattt
     6781 ccattctttt tccatgaagt acatcttcca tggtgagaga agtttcatat aacatatcac
     6841 gaccacgttg gtttccaaaa ccaccaaagc ctcctcctcc tccaaacagg ttttgaaaaa
     6901 tagaatcaaa acctcctgat gatctacctt gtcttccaaa caaatcacta aatacatcat
     6961 caaagttggc tccagctcct cgaaagatat cttcagtact atatctacca tctaccccag
     7021 catgaccgtg ttgatcataa agactacgtt tttttggatc ggataaaact gcatatgctt
     7081 ctgaaatttc cttaaaatgt tccccagcct ctggtgattt gtttctatct ggatgaaatt
     7141 ttaatgctaa ttttctatat tgagacttga tctcatttat tgaagcgttt tttgagactc
     7201 ccacaacttc ataataatca cgttttgcac tcataatata tcaaataatc ctttactgta
     7261 taaaatatct aatgtatata tttttgaata gttttaattt gtatatcgtt atttagactg
     7321 ctcttcagtt ggttttgatt ctggttcaga ttttgtattt tcttcagatt ttgtattttc
     7381 ttcagatttt gtattttctt cagattttgt attttcttca gattttgcgc cgtcatcttt
     7441 ttgctgatct gcacctggtg gtggcgcagc atttttgtat atttctgtag ttatctcatt
     7501 aactattgct tttaatgcat caagttttgt tttgatttcg tcttgatctt tagttagaga
     7561 ctccttcaat tccttcatag catcagtggc tttgattccc tgttcctgtg atattttatc
     7621 cttcaaatct tggttgatca atttttcagt agtgtagata tagctttctg cttcattttt
     7681 aatatcgact gactctcttt tcttcttgtc ttcttcagca tgttcttctg catctttctt
     7741 cattttttca acttgctcat ccgataattt tgtagaagat tctattgtaa ttttagcatc
     7801 ttttccactt ccaagatctt ttgctgttac atttatgatt ccattagcat caatatcaaa
     7861 tttgacatta atttgtggaa cacctctggg agctggtaaa atctcagtaa gattaaaact
     7921 gcctaatgaa acattatctg caaccattgg acgttcgcct tgtactacat taatcgtaac
     7981 agcagtttga ttgtcagcag ctgttgtgaa agttttatct ttagatgtag gaattgttgt
     8041 atttctctcg ataattggtt cacgtacacc acctaatgtt tcaattccta atgttaatgg
     8101 agttacatca agtaatacaa tatcactttc aacatcacca gccataatac cagcctgaat
     8161 agcagctcca aaagcaacag cttccatagg atcaactcct gattctggat ttttcccaat
     8221 agcgttttca acgaattttt tcacaatagg cattctagta ggtccgccaa ctagtacaat
     8281 cttggtaata tctgaagttg atattttggc atcctcaatt gctttatcga ttgaagattt
     8341 acatttttca actaatgatg ttacgaggct ctctatctta gctcttgtta atttcacttg
     8401 aagcgtagtc ttttcatgga aatatggcaa gtttatttca gtttccatta cacctgaaag
     8461 ttcaatctta gccttttcac aagcttcttg gagtcgtgcc atggcttcct tatcacttga
     8521 aagatcaata ccttcttttt ttctgaattc ttctttgaca taatccatta ttactaaatc
     8581 catatcagta ccacccagat gagtatcgcc agaagtgctc attacttcaa agacaccacc
     8641 tcccatttcc attattgtta catctagtgt accaccacca aaatcaaata ctaatatttt
     8701 catatcttgc ttcgctttgt caagaccaaa agcaagagcg gctgcagtag gctcattgat
     8761 aattcgtaca acatccaatc cagctattgt tcctgcatcc ttggtagctt gtctttgatt
     8821 atcatcaaaa taggcaggaa ctgtaattac tgcttttgta atcttatcgc cagtaaaggc
     8881 ttcagcatct ttcttaattt tttgtaaaat aaacgcagag atttgttgtg gtttgtattc
     8941 tttaccttga attttgtaaa tatgatcttc acccatcttt ctttttgcct tagtaatagt
     9001 attttcagga tttgttacag cctgatttac agcaggagaa ccaacaatca tggggctacc
     9061 atcctttgga aatgcaacaa cagatggaaa tgctttaccg tgtgctgttt gaccctcagc
     9121 agcaggaatt atcgttggct taccgccttg gataactgca gcagctgaat tgcttgttcc
     9181 aagatcaatt ccaattattt tttctgacat ttttttatac ctattttttt attatttttt
     9241 tcgaaacaca tactttcgag tatttcaata ctttgccacg aataatatat ccttttgcga
     9301 tctcttctat gattgtatct tctgctagtt ttggatcttc tactatagaa atagcttcgt
     9361 gtagatttgg atcaaaaatt ttacccatag cgtctattgg ccttacatca tattctgaaa
     9421 atagagaatt catattttta ataataccct ctagacccgt aatatctact ccatcttttg
     9481 catatacctc cttagcacgt ataaaatttt catatacagt taaaaattct aaaatgagtt
     9541 taccagtttc cgaactaact ttatcaataa tttcttgctg tgttcttttt tcgaggttgt
     9601 cgtaatcagc tagagcacgt ttccattttt ctgttgtttg atctactcgt tcttttaatt
     9661 catcaatttg taatttatgt aaagtctgtt ctgattctgt agtttctatt tcagattcat
     9721 cataatgttc taaattttta ttatcatttt ctgcatcagc ctgtgataca tcgtttgtat
     9781 tttcgttaat gtgtgacata tctttcaaat tttcattaga taattttact ttttgttttc
     9841 tatctcgaaa agggggtttg ctttgataat aataagatct gcacttctct tatctgaaac
     9901 aatttttgtg taatcattca tagagcaggc tataatgata gtttttttgt cactgtgaaa
     9961 ggagtttact aatccttcag atgtatccac atcaccaatg taatcacgta atttagatgt
    10021 aatagaattt aatatctcaa tcttatctcc cctcatttca tttttatcta gtgtccaaga
    10081 taacacaatt tttgttctac tagctttcag ttcatttttc tttagggtag tcctaattgt
    10141 atcaattaat cttttgatat aactgtgaat ggccttgata cttgcatttg ttaaatatag
    10201 taaattgttt gctccctcaa atgaagatcc aagtaaacgt aaatcgtaaa gaccttttat
    10261 tatatcatca aggtatacag ttttaaaatt attaaatgtg gtagattttg tatctttaac
    10321 aaatttacaa atttctaaaa ttgcagaacg atttgtgaaa tattgtataa tattgatacc
    10381 gtaaatacat tctgcagaag taattgatac aaattttttt tctttttttt ctattgttaa
    10441 aagagatgat agtatcttgt catcttgatt atctaatgaa ccaattactt caggtgtttc
    10501 atccaaatta tttaatggaa aataaaaata tgagattgtt ttgcccacat ccaatccagt
    10561 aatatgtgtt aatagtgata tattctcttg gtttcctccc agcccagttg gaagacaata
    10621 aattatcgag cttccttttt tcagtgcttt tgttgcatct ttgtattttg aagtaatttc
    10681 tgttctgaga tcctgaccag tttttcttat tcttggtgca aagaaaagat acttcgcatt
    10741 tgaaatagct acatcaatag gttcgacaga aagtagaggc tcatcttctt gaaatgtagt
    10801 aagattagga tatgtcttgg cagtttcagc ttttagtagt actgctgatg gagatgattc
    10861 atctatgata gaaacatctg ctcctttcat agccatttgt gatgcaagaa agtatccttc
    10921 agtagttaat ccataaacaa caattttttc ctttttcatt gtaattaagt ggtataaagg
    10981 gggagaaata cttattgcat ttcagaaatc agtgaaaatg cttgaaaatc tggcttgata
    11041 tacttacgcc caaacaaata ctattttttg aacccatgat tcaacgtctg aaaaaaaatc
    11101 atagtgtatt atgcacttca agaaaatata atcaagtgac tgatttagca aaaatacgga
    11161 agcaaaagtt agtaataata ggaaaacatg gtggcggaga aaaacatgat aaattgaatg
    11221 ctagtttaag ccgttcaaaa ttgctagtta aaaaaattag caagttttcg cctgacatta
    11281 caataagttt ttgctcgcct gaagcagcaa gaatttctta tggtttgggc attaaacata
    11341 tttgtttttc tgattcacct catgctgaaa atgtaatgcg tcttgttgta ccattagtac
    11401 aaaaattact aataccttgg attataccga agaaaaaatt tacaaaattt ggaattgatc
    11461 agaatgatat aatacagtat aaagcaattg atgcagcaac aatttcaaaa cgaaagataa
    11521 aaaatattaa aaaaactgtt ggtaaaagaa taatactagt tagattagaa gaagatgagg
    11581 cagcatattc attaaaaaaa agacccataa tacctatcat caaggaaata ctcaaagaat
    11641 tttctgggca ggagattgta attatgaccc gctatgcgtc acagaaaaaa tatcttcaac
    11701 aaatttttca gaaaaaaatt aagatactat ctaaagttgt tgatagcaaa ttacttttat
    11761 ataatgctga tatttttatc gggtctggag gaacaatgac tgctgaatct gctcttcttg
    11821 gcattcccac tatttcatat gatgcggttc caaacattat tgaagattat cttgtgagga
    11881 aaaaattggt aatgagggag gtaaatccta aaaaaatggt cagtgtgatg aagaaagttt
    11941 tcaaatcttc taattcgaga agaaaaaata gagctaaaaa gatgatgtct tctatggaag
    12001 atccttatcc cattcttcta aaaaccatga agtctatctt gaaataatgt tataagggga
    12061 aaaggaagaa aaattacgaa gcccggtagt gtagtggcaa gcataggggc ccttggagcc
    12121 tttgacctcg gttcgagtcc gggccgggct atcatttttt taaaaatcca ttttagtcaa
    12181 taatttggtt gcaataataa aaagaataga tgcaagtgtt atcaaaacca ccatttctaa
    12241 aataacaaat tctgtaatgg tgccaaaaac tccagctctg attatgtcta caagatatgt
    12301 taatggattc aaataaaaaa tagttgcaag aggttgtgga gcaccggcag caggataaaa
    12361 agctgtgctc acaaaagcaa agaaaataaa aactgtgttg ataattacat tgaagccttc
    12421 acttgaacgt aatcttgctg atatgatcga agcaatggaa ccaaataata ttgcacccac
    12481 tattgaactg aaaattataa gtggaattgt gattaaacta aactctgctg attgaaaaaa
    12541 tactggataa ccaactaccg tgattaatgc tgcactaact aggccaacta ttgcaatagt
    12601 tgtaatatta ctaagaatgt aatttgctcg tgtaaatgga cctgacatta tttgttcaaa
    12661 cattccatgt cgtctatcat tccaaattat aattccagaa acgagtgtac tattcataat
    12721 attgaaacca atcatcccag atgctaagaa tgctggatag cttagatctt tgtttccaaa
    12781 agatacttca tctattaatg cagtgtatgc aaaaccagct acaaaaatcc aaatcagtgg
    12841 aaaaattacc tgccagatca aaaatcctgg gttaatcgaa attgtaatgt ttctattaac
    12901 taatctaatt atcgggtgca ttggaatcac tcacaacttt cagaaatatt tcttcaaggt
    12961 ttgttggtat tgctgataaa tcatctatag ctatattatt ttgatttaaa atttttagaa
    13021 tattcatcaa aacaacttca gaattagtag accgtatagt gattgttgca ccagtaccat
    13081 agtcaatttt acaatcttga atattttcta aaagatctgg taacgtttca gtgttagatg
    13141 aaatatgaat tttaattgtt ttttccttac caaatctatt tttcagctcg tttggagaat
    13201 caacggtaat tatcttacct ttgttaatta ttgcaatatt atcacaaaga tattctgcct
    13261 cagttagtac gtgtgtagta taaaatattg ttagattctt ttcctttact ttgtttttca
    13321 aaaaatctaa taattgccgt ctagccgttg gatccaatcc aacagtgggt tcatccaaaa
    13381 atagtaaatc catatcatgc ataaattcac gcgccacctg aactcttcgg cgttgaccta
    13441 tggacaggtc ttcatttctc ttctttctaa tttcgaccag attaaaagca gttaacagtt
    13501 cttcggttct agccttccta gtttttttat ctatattcca catcatacca tacttatcta
    13561 gagatttttc cacagaaagt gttggctcat aactgggttg ctgcaaaacc acaccaatct
    13621 tttttcttat ctttaatggt tccttcatag cattaatttt tagaactgtg atttcaccac
    13681 ctgttggctg aatcaatgta gttaacattt taatcacagt ggatttgcct gctccatttg
    13741 gtcctaaaaa accaaaaatt tgcccctcgt cgacatcaag atctatacca tctactgcgt
    13801 ttataccatc atatgacttg aaaagggatc ttgctagaat tgatttcata tttcttaaaa
    13861 ttctccaact cgccatatat gttaataaga gattaaaaag taaaattagt gactagcatg
    13921 tctgagtttg aagaaataat agaaaatata ctaaaacaaa aacctgaatt aactagagaa
    13981 aatttagaag ggttgatcga tcaaaaaacg caaaagatag gtgcgggata cttaactgat
    14041 caaggagccc tattcctgat tgcatctgac ctaggaattt cattgacaca atctactaag
    14101 ttagaggttg gactcaagga tctttatgtt ggtgctaaag agatatcgtt ggaaggaaga
    14161 gtgttgaata tttcccctac aaaagaattt tctagaaagg atggttcctc tttcttacta
    14221 agaacaatta ctgtgtatga taatgattct accgcaagtg taaagctttg ggatgataaa
    14281 gctaatcttc ccggaattga aaatttgaaa cctggtgatt tagttaagat aatcaaggct
    14341 tatgtaaaat cagatcttaa tggaagtcca acgataaata ttggttcagg ttctgatata
    14401 gaaccaataa atcaagaaag taaaatccgt tccattgatg ctctagctgt tgactcgagt
    14461 gaaattaaac aggaccaaaa taatttagtg atttctggta aaatagatgg tagtgtaagt
    14521 acattagaat ttactaacaa acgtggagac ccagcaaagg cgcttaaaat gagtttaaag
    14581 ggaaataatg gtgcttcaac aaaagtaata ctatggggaa aagatgaaac atctttgcct
    14641 aaagttgttt ctcaagattc aaaagttcta cttttgggtg tgagaacaaa aatgggaaat
    14701 caaggtcttg agattcacgg aaacgatgga acaatggtta aagttgaagg gagtaaggag
    14761 attgagccta ttattcttcg tatcgtcgca aaaactaaaa atgagcaagg aaaaactatc
    14821 gccttaggaa ttaatgataa gaaaaatatg gtttatgtat ctgattccgt aaatatgtta
    14881 gagcccacca atattggtga tgttgtagaa tgtatgccat cacaagttta tggaaattct
    14941 ctgataattg actcgggctc gttagtaaga aatattgaag atgatggaac actttctaca
    15001 ttatcagatc taaggacaaa aatttctgag attaaatctg gaaatgaata ctgtgttgaa
    15061 gcaataattt tgaaggaacc agaaagacga gatgtgcaga caaaagctgg agaaacaatt
    15121 tcattaggtg aaatgtttgt agaagatact actggacaga tctggattaa gggttggaga
    15181 aatcaagcta aacttttaga tggattatca agaggtgagg taatttctgt caccagtgtt
    15241 aatgcaaagg ctggtcttga aggtagaact gagttgtttt tgactccatc ttcaactgtt
    15301 aataaaaaat ttaatcatcc caaaaacccc tagtcattac atactgtctt tcagcttcgt
    15361 aaatctgttt atacatatac tcctcatcaa ccatgtttct tagaatacgt gctgctgtat
    15421 cagcacctac accatatcct gataaaacag tgattgcatt attaccaaat gtttcaatta
    15481 atgaagcaac cttccaagct cgtttaaatt tatgattttc ttccaatgaa agtttcttgg
    15541 atttatggtt tttctgaatt attttcacca agtcatagtc ggaataataa gtggcagtaa
    15601 tttgtcttcc cttacaatat ttacagatca gtttttttgg tcttttctca aattctcctg
    15661 taattatagc tagttgccat ttcccacaac gagcacaaat tagacgatgt ttagttttta
    15721 gtaagcgttt ttttacctca tcaagtattg ctttatccac atttgctggt gatgaataat
    15781 attttgtagt gtgatctaaa agtggttctg ccaattttga aaatttgaca acatctatcc
    15841 attcaatttg tatttcatta tttttgattc tggtcaagag tttatctgtg cctaacaaat
    15901 caaatttatc atggaatagc tcacgtaatg cttctttaac aagtggtgtg gtttgatatt
    15961 gctcataaat gaaatgccca gtttttctat cataaataga tccacgttct accataccaa
    16021 atttttttgc aacacaccat gttctccaat taatattatg tgt
//
LOCUS       DASCPJ010000026         2722 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000450345, whole genome shotgun sequence.
ACCESSION   DASCPJ010000026 DASCPJ010000000
VERSION     DASCPJ010000026.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000450345"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            202..1425
                     /gene="pgk"
                     /locus_tag="QGF01_01465"
     CDS             202..1425
                     /gene="pgk"
                     /locus_tag="QGF01_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HJN19600.1"
                     /translation="MRILTIDDLEFDNKTVFLRVDMNCPIDSQTLEISGTKRIEETID
                     TLNSLKNAKIVIASHQGRVGNKDYTSMAKHAKILEKLLNRKIKYTDDIIGTTAQNEIK
                     NLESGDILLLDNLRLCAEENYEFTGSTAAKTIMVQRLAKLFDICVLDSFPSAHRSHPS
                     IVGFSYILPACAGRIVEREVMKLDEILSVAKAPHTIVLGGSKIDDRLEAIRLLIKNGR
                     ADHILLTGLIGNVFLRAQGRIKSSLGIKREEEMVSNAHSLIGEYPDIFSTPVDVAIEK
                     NGKRVELDVRDLNKDDQVYDLGPKTIEYYNKIISGAGTVFISGPPGFFEKENFDYGTK
                     TLLECVTNSMATTIVSGGHLTSALKKYGLEKKITHISTAGGALVRYLTGIKLPMIKSL
                     EESAEKYRSTVKNHN"
     gene            complement(1403..1738)
                     /locus_tag="QGF01_01470"
     CDS             complement(1403..1738)
                     /locus_tag="QGF01_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19601.1"
                     /translation="MDNIPITISIRKIIFENFNDDDLRFNNDQIFEILKQNKIIESSL
                     TIDDMEIYFKELCDSEILRNIAQNFTTQWFKLFESLNKVQCVSCKEDSYLISSENKIC
                     QNTSCGSSL"
     gene            complement(1740..2189)
                     /locus_tag="QGF01_01475"
     CDS             complement(1740..2189)
                     /locus_tag="QGF01_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HJN19602.1"
                     /translation="MSGDPIYTEMIIEYSRNPSNFGKIENPQIHRHDSNPLCGDSIDL
                     YVNIDDSKITEVKFDGKGCAICMACTSVLTEMIQGKNLDDVKNFQKDELLAELGLEHL
                     IKTSPVRIKCALLSLKALKHGIYSYFAEKMNDVEAAGNLKEEAASLY"
     gene            complement(2186..>2722)
                     /locus_tag="QGF01_01480"
     CDS             complement(2186..>2722)
                     /locus_tag="QGF01_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJN19603.1"
                     /translation="GVGILWARKELLENMKPFQCGGDMIREVHKQETTWNDLPYKFEA
                     GTPNVADVICFAAAIDYLTEIGMDNVRNHEIELTKYALEKMSKVKGLVIYGPSDTKKQ
                     GGVISFNFKDVHPHDVATIIDKNGVAIRSGHHCAQVLMEKLDVAATNRASFYIYNTKE
                     EVNTLIDSLEQVAKVFKL"
BASE COUNT          933 a          431 c          433 g          925 t
ORIGIN      
        1 aaaaaaaacc aattgaacgc atttcgaaga taaatttttg catcaacgaa ctctcataac
       61 gatcattttt aattttgtct atgaattaag tgatcgtacc caaaatcagt gctgtttttt
      121 ataaaacata aacagaacac aagtttactt tgaaattatg aagttgtaaa agattttaac
      181 tggattcact ggatggtata tgtgaggatc ctaacaatag atgatttaga atttgataat
      241 aagacagtat ttttgagagt cgatatgaat tgtccaattg attctcagac tttagaaatt
      301 tctggtacaa aaagaattga agaaacaatt gatacgttaa attcattgaa aaatgcaaaa
      361 attgttattg catcacatca gggtagagtt ggaaacaaag attatactag tatggctaaa
      421 catgccaaaa tacttgaaaa attattaaat cgtaaaatca agtatactga cgatattata
      481 ggtaccactg cacaaaatga gataaaaaat ttagagagtg gtgatattct gctacttgat
      541 aaccttagat tatgtgcaga agagaactat gaatttacag gatctacagc agcaaaaaca
      601 ataatggtac aacgattagc aaaattattt gatatctgtg tcctagattc ttttcctagt
      661 gcacatagat cacacccatc aattgttggt ttttcatata tactaccagc atgtgcaggc
      721 agaattgttg aaagagaagt gatgaaatta gatgagattt tatcggtagc aaaagctcct
      781 catacaatag ttttaggtgg ctcaaagatt gatgataggc ttgaagcgat acgtttactc
      841 ataaaaaatg gaagggcgga ccatatttta ttaactggat taatcggtaa cgtgtttttg
      901 agagcacagg gtagaattaa atcaagtctt ggaataaaga gagaagaaga gatggtttca
      961 aatgcacatt ctctgattgg tgaatatcct gatatttttt caactcctgt ggatgtagct
     1021 atagaaaaaa atgggaagag agtagagtta gatgttagag atttgaataa agatgatcag
     1081 gtttatgatc ttggacctaa aacaattgaa tactataata agattatttc tggagctggt
     1141 actgttttta ttagtggtcc tcctggattt tttgaaaaag aaaattttga ttatggaact
     1201 aaaactctac tagaatgtgt aactaattct atggccacaa caattgttag tggtggtcac
     1261 cttacatccg cattaaaaaa atatggatta gaaaaaaaaa tcacacatat tagcacagct
     1321 ggtggtgcat tggttcgtta tcttactggt atcaaactac caatgattaa atcgttagaa
     1381 gaatcagcag aaaaatatag atctacagtg aagaaccaca actagtgttc tgacatatct
     1441 tattctcaga tgatatgaga tagctatctt ccttacatga tacacattga actttattca
     1501 gtgattcaaa taacttgaac cattgtgtag taaaattttg agcgatatta cgtaaaattt
     1561 cactatcgca taattcttta aaatagattt ccatatcatc tattgttaaa gatgattcaa
     1621 tgattttgtt ttgttttaaa atttcaaaga tttgatcatt attgaatctt agatcatcat
     1681 cattaaaatt ttcaaaaatt atcttcctaa tactaattgt tattggaata ttatccatac
     1741 tagtaaagtg atgcagcttc ttcttttagg tttccagctg cttccacatc attcattttt
     1801 tcagcaaaat aagaataaat cccgtgtttt aacgccttca aagaaagtaa tgcacattta
     1861 attcgtactg ggcttgtttt gatcagatgt tctagaccaa gttctgccaa taattcatcc
     1921 ttttgaaagt tttttacatc atcaagattt ttgccctgta tcatttcagt taaaacagaa
     1981 gtacatgcca tacatatagc acaacctttg ccatcaaatt ttacttcagt aatttttgaa
     2041 tcatcaatat taacatacaa atcaatactg tcaccacaaa gaggattact atcatgccta
     2101 tgtatttgag gattttctat tttaccaaaa tttgaagggt ttctagaata ctctattatc
     2161 atttctgtat aaattggatc tccactcata atttgaacac cttagctacc tgttcaagtg
     2221 agtcaattag agtgttaact tcttcttttg tattgtaaat gtaaaaactt gcacgattag
     2281 ttgctgcaac atccaatttt tccatgagta cttgtgcaca atgatgacct gatctaattg
     2341 caacaccatt tttatcaatt atagttgcaa catcatgtgg gtgaacatct ttgaaattga
     2401 atgatattac gccaccttgt tttttcgtat cactaggacc ataaatgacc aagcctttga
     2461 cttttgacat tttttctaat gcatactttg ttaactcaat ttcatgattt ctaacattat
     2521 ccattcctat ttctgtaaga taatcaattg cagctgcaaa acaaataaca tccgcaacat
     2581 taggagttcc agcctcgaac ttgtatggaa gatcattcca agttgtttct tgtttatgaa
     2641 cttctcttat catgtcacca ccacactgaa agggtttcat attttctagt aactcttttc
     2701 gtgcccacag aataccaaca cc
//
LOCUS       DASCPJ010000027         3207 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000463546, whole genome shotgun sequence.
ACCESSION   DASCPJ010000027 DASCPJ010000000
VERSION     DASCPJ010000027.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000463546"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            199..348
                     /locus_tag="QGF01_01485"
     CDS             199..348
                     /locus_tag="QGF01_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19604.1"
                     /translation="MRFPSIVLQTISSAKNYALDSTEPKRGKKYRLLSDIEKIVYMMK
                     DIYYY"
     gene            463..1719
                     /locus_tag="QGF01_01490"
     CDS             463..1719
                     /locus_tag="QGF01_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019615910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HJN19605.1"
                     /translation="MKILIVNTSDIDGGAARASYRLHKSLLALHVNSKMLVQNKISCD
                     FTVIAETSKLRKGFNKLRPTIDSLPIGFFKERTKTLFSPSWFGFSNIVDHINEINPDI
                     VHLHWICGGMMTIEDIARIKAPIVWSLHDMWAFTGGCHYDEECQGYEKECGNCKVLGS
                     DKENDLSKKIFKRKQKAFTQKKDITIVGISNWLNECSKNSTLLKDKNHINLPNPLDTN
                     IFKPFDKEKARELWGLPKDKKLVLFGAMGATSDPRKGFYELMESLEKIEDSNVELVIF
                     GSERCEDTKDLGFNVYYLGQLYDDVSLVTLYNSVDVMIVPSLQENLSNTIMESLACGV
                     PVVGFDIGGNADMIDHERNGYLAKPFSTIDLAFGIEWVLNNYNYKILCQNARNKVLNN
                     FDSVILAEKYIELYKSILDPINEIVC"
     gene            complement(1767..2684)
                     /locus_tag="QGF01_01495"
     CDS             complement(1767..2684)
                     /locus_tag="QGF01_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19606.1"
                     /translation="MKHDALQTNPPVLVDIGASGTIHEKWEPIAKYAICIAFDADSRD
                     FEICESEDKGWRKLYSLNRLVASEASEEMDFYLTHSPHCSSSLAPDKEALKPWAFSPL
                     FEVENIVKLPSVDLQSALLKAGVDYIDGYKTDSQGTDLRIFNSLPTEIIDKMLIAEFE
                     PGIIDAYKGEDKLHHLLAHMEKESFWISKMEIKGSQRINHLDFKSLNYFQQRGIGSFL
                     KMAPGWCEISYLNKMEIGEMTLREYLLAWVFASLSSEHGFALFVAKKGYELFGDKLLL
                     NLQKKSLKKLSSGYFHLSFKLLKRIAKLK"
     gene            complement(2876..>3207)
                     /locus_tag="QGF01_01500"
     CDS             complement(2876..>3207)
                     /locus_tag="QGF01_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19607.1"
                     /translation="TAWIKEMKMMWYVDRIRKIENNFSKLSDLQITLLLQLINSDILA
                     SVEKISPKIKLNIKGSIEDFIIYRSERGFEGEDYLQLIDDPNFRNDSAPHIKKLISRH
                     EYLTSMH"
BASE COUNT         1090 a          473 c          574 g         1070 t
ORIGIN      
        1 taacgacacg taaatcttcc aaatatggtt cataaatctg ttttcttgtt attgaaggaa
       61 tggaatacga agtggtaaat tgaaccatgg gttttgatcg tttttctgtg agacgaagtt
      121 catgaaagtg ccaaaaaatt ccgtcttaac acttaaagaa actatggaat atatagtaat
      181 gcatagttca atataggtat gaggtttccc tctattgttt tacaaactat ttctagtgct
      241 aagaattatg cacttgattc gacagaaccg aaaagaggta aaaaatatag attattgagt
      301 gatattgaaa aaatagttta tatgatgaaa gatatatatt attactaggc gataagaaat
      361 aattgagata tttaaagata cagatgataa atttattaga aatgaagatg atacagtgaa
      421 aagattgagt atacatactg aaaaattaaa agatatttta aaatgaaaat tcttatagta
      481 aacacatcag acatagatgg tggagcggca agggcttcat atagacttca taagtcattg
      541 ttagccttac atgtaaatag taaaatgttg gttcaaaata aaattagttg tgattttaca
      601 gtaatagcag aaacttcaaa actaagaaaa ggctttaata aacttcgtcc aactattgat
      661 agtttaccaa taggattttt taaagagcga acgaaaacac tttttagccc atcttggttt
      721 ggctttagta atatcgtaga tcatataaat gagataaatc ctgacatcgt acatcttcac
      781 tggatatgtg gagggatgat gactatagag gatatagctc gtataaaagc tcctatagtt
      841 tggtcacttc atgatatgtg ggcttttaca ggtgggtgtc actatgatga agaatgtcaa
      901 ggatatgaaa aagaatgtgg aaattgtaaa gttcttggaa gtgataaaga aaatgactta
      961 agtaaaaaga tctttaaaag aaaacaaaaa gcatttactc aaaaaaaaga tataactata
     1021 gttggtatta gtaattggtt aaatgagtgt tccaagaata gtacactttt aaaggacaaa
     1081 aatcatataa atctaccaaa tcctttagat acaaatatat ttaaaccgtt tgataaggaa
     1141 aaagcaagag agctttgggg tttgccaaaa gataaaaaac tggtgttatt tggtgcaatg
     1201 ggggctacaa gtgaccctcg caaaggtttt tatgagttaa tggagtctct agaaaaaatt
     1261 gaagatagta atgttgaatt agttattttt ggcagcgaaa gatgtgaaga cactaaagat
     1321 ttagggttta atgtatatta tctagggcag ttgtatgatg atgtgagttt agttacactt
     1381 tataattctg tggatgtaat gatagtgccg agccttcaag aaaacttatc aaatacaatt
     1441 atggagagtt tggcttgtgg tgtccctgtt gttgggtttg atattggtgg aaatgctgat
     1501 atgatagacc atgaacgtaa tggttattta gcgaaaccat ttagtacaat agatttagca
     1561 tttgggattg agtgggtttt gaataattat aattataaaa tcctttgcca aaatgccaga
     1621 aacaaggtat taaataattt tgatagcgtg atactggctg aaaagtatat tgaactttat
     1681 aagagtattt tggaccccat aaatgagata gtatgttaag gtgtattttc aatcatttag
     1741 gattactatt ctggaatagt tattcattat ttcaattttg caattcgttt taacaattta
     1801 aagctgagat gaaaatatcc cgaagataat tttttcaggg attttttttg taagtttaat
     1861 aggagcttat ctccgaaaag ttcatacccc ttctttgcaa caaacaaagc aaatccatgt
     1921 tctgaactga gcgatgcaaa aacccacgcc agtaaatatt cgcggagtgt catttcacct
     1981 atttccattt tattgagata ggaaatttca caccacccgg gggccatttt caaaaaagat
     2041 ccaatacctc tttgttgaaa ataatttaat gatttgaaat ctaaatgatt gatacgttgc
     2101 gaacccttga tttccatctt tgaaatccaa aaagattctt tttccatatg cgccagaaga
     2161 tgatgtaatt tgtcttctcc cttgtaagca tcaattattc ccggctcaaa ttccgcaatc
     2221 aacattttat ctataatttc tgtgggtaaa ctgttaaaaa ttcttaaatc cgtgccctgg
     2281 ctatccgttt tataaccatc gatatagtcc actcctgctt ttaagagagc actttgaaga
     2341 tccacagaag gtagtttaac tatattttcc acttcaaaaa gaggactaaa tgcccatggt
     2401 ttcaaggcct ctttgtcagg agctaaagaa ctggaacagt gcggtgaatg agttaaataa
     2461 aaatccatct cttctgatgc ctcagatgca accaaacggt taagagaata gagttttcgc
     2521 catcccttgt cttctgattc acagatctca aaatccctcg aatctgcatc aaacgcaatg
     2581 cagattgcat attttgctat gggttcccat ttttcatgaa ttgtacctga agccccaata
     2641 tctactagaa caggtgggtt tgtttgtagg gcatcgtgtt tcattatttt atctataatc
     2701 aatctcaccg atcggacctg tggtgagatt tgggacttac atctatcatt ttatctttac
     2761 aacaagatcg atggtttaac cacttttatc tagaatttat atcaaacgtt tgtatctctt
     2821 attaagagtt aacaacttat aaaaaatccg atcattcaga tttgccatat acattttaat
     2881 gcatcgacgt caaatattca tgtctggaga taagtttctt aatatgtggg gcgctatcat
     2941 ttctaaagtt cgggtcatct attagctgta gatagtcctc gccttcgaaa cccctttctg
     3001 atcggtatat aataaaatct tctatcgaac ctttaatatt taatttaatt tttggagaaa
     3061 tcttttcgac agacgccagg atatcagaat taattaattg taataatagt gttatttgca
     3121 aatcagataa ttttgaaaaa ttgttttcga tctttcttat tcgatcaaca taccacatca
     3181 ttttcatttc ttttatccaa gcagtaa
//
LOCUS       DASCPJ010000028         7532 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000468379, whole genome shotgun sequence.
ACCESSION   DASCPJ010000028 DASCPJ010000000
VERSION     DASCPJ010000028.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000468379"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(127..366)
                     /locus_tag="QGF01_01505"
     CDS             complement(127..366)
                     /locus_tag="QGF01_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJN19608.1"
                     /translation="MSVAYVLVQCKIGYEMKILKELLSFHFVKEAKGTFGFYDILVKI
                     EDQSEKKIEHNITKDIRQINNVITTTTLSVIPEQD"
     gene            complement(363..1802)
                     /gene="purF"
                     /locus_tag="QGF01_01510"
     CDS             complement(363..1802)
                     /gene="purF"
                     /locus_tag="QGF01_01510"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HJN19609.1"
                     /translation="MVKENCGVVGIHSLDGVNVIPMLIDSLRALQHRGQEAWGIAVPN
                     KSPLKKLGLVSSSASEFKKISMEYSSASAIGHVRYSTIGKSNIDNAQPLKVRDLCVAH
                     NGTISNVEELSNVVGGCSFTPQNTSDTMIAAQRLVSLIKEKGQMSKALSILKNEMIGS
                     YCFTFLSDDTSVYAARDPCGFRPLVLGEKNDGRVHIVASESSAFSAIGAELIRDVSPG
                     ELIKFSKNGIESQSFSEDKTHAHCSFEFTYFAHPTSKMEGTNIYVARKRIGKYLARKF
                     PIKDADLVIPVPDSARPAALGFAQELGVPFDEGLLKDRYSKKGPLRSFIEPHQTDRIE
                     INRWIIPIREIIENRHVVVVDDSLVRGTSSKAIIKALRKAGASKITMLITYPPIKFPC
                     YAGIDFPSQDELATYIDDKKYSEEEIIENVRRDIGADFLGYNDPENLANAIGIQKDSL
                     CFTCTTGNYTSLGITPVFKTREEMKGEVQ"
     gene            complement(1795..3963)
                     /gene="purL"
                     /locus_tag="QGF01_01515"
     CDS             complement(1795..3963)
                     /gene="purL"
                     /locus_tag="QGF01_01515"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HJN19610.1"
                     /translation="MSLSVQESKHITKKIGRDLSITERQIIAAEWSEHCSYKSSKKHL
                     KILPTTGRNVINEKGYDSGVLDVGDGYVVTVHIESHNHPSAVEPYGGAATGVGGVIRD
                     ILSTGTRPIAIFDGLRFGDIENDTHARWLFKNAVSGIADYGNCLGIPTIGGEVEFDEC
                     YKNYALVDVAAIGFGKKDKLIKNHANVGDLVVLIGGPTGRDGIGGSQFASDSLESEDR
                     SAIQIPDPFIEKLIIETILEARNEKCINAMKDLGGGGLSCAISETADSLGIGIELDVD
                     NIHTREAGLSPDEIMISESQERMLIITEKKKLPKLNEICNKFRVSCSLIGYVKNDKMM
                     HVRKGENTFALLPADFVAHAPLLDRKKTIPKYLRHLKKENKNKKLLDYSKTILKLLSS
                     PNIASKHWVFHQYDHEVGIRTVVKPGFDASVLRLDNGKFLSAKIDGNPKHCYLNPRQG
                     AIGCFEEACRNIVCTGANPIGMVDHLQFGNPEDPEIFWTFMEALEGLSDFAKFLRVPC
                     VGGKVSFYNETPSGPIKPTPLIGVLGIIDKPPLVPVPPSNGDYLLIVGKTKDELGGSE
                     YFEYVHKFIGGNCPTVDFKTSKLNMITVLSLIKNNLIKCVHDCSKGGFVIALSELSIN
                     GNIGCSIDIEKIPSTKNLSHEKILFSESHSRYLLVVDGKNITRVKQYLSKQKILFGVL
                     GKFSGDQISITHKSNNLVKSNVDVMRKRYFNGLEDLLKHG"
     gene            complement(3960..4640)
                     /gene="purQ"
                     /locus_tag="QGF01_01520"
     CDS             complement(3960..4640)
                     /gene="purQ"
                     /locus_tag="QGF01_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HJN19611.1"
                     /translation="MKVGIVVFPGSNCDRDIFHVLTDVFDLEAEYFWHEKHLPSKIDA
                     VILPGGFSYGDRLRAGVIAAHSPIISDVKSMAKKGVPVLGICNGFQILVESGLLPGVL
                     LKNTTMNFMCKWINLIVQNNNTPFTNKLKQGQKISIPIANGEGRYFADNKTLKELQEK
                     NQIVFTYEESLNGSSLNIAGVCNESGNVVGMMPHPERAVESEINPTDNKPSSLIFESL
                     LKTAGIEK"
     gene            4712..5125
                     /locus_tag="QGF01_01525"
     CDS             4712..5125
                     /locus_tag="QGF01_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJN19612.1"
                     /translation="MGQVRDIMEKNVITIDINETANNAANQMKENDISFLVIIKNGKP
                     VGVVSERDFVQKLCINNQNSSDVKISDIMSYKFRWVNPTTKIEDAIQKMLNNNIRRLL
                     VLDDEKLVGVITQTDLASYLREKLMIDGTIKNIKK"
     gene            complement(5127..5387)
                     /gene="purS"
                     /locus_tag="QGF01_01530"
     CDS             complement(5127..5387)
                     /gene="purS"
                     /locus_tag="QGF01_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HJN19613.1"
                     /translation="MPTYWVHVTIENKSGISDPEGETILNDLVLQGKFSSVSKINSAK
                     LLKFQINEKNKKLAKKKIEQLCDELRIFNPMVSKLSINIITD"
     gene            5463..5927
                     /locus_tag="QGF01_01535"
     CDS             5463..5927
                     /locus_tag="QGF01_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19614.1"
                     /translation="MSRSRYWKLTSEEVENLTHDPDKILNWEIKCIKEPEVDAKFIGV
                     FLYRHGTPYDYDSKKGIVYYYNNIERNDLPEITKFLQKRFGGEKIEKGERIFLEGSKE
                     IYAGKEIADLANALNEKFHTKSVISMELDGATQDELKGWGYPDSKLLPIPGK"
     gene            5961..6752
                     /gene="tatC"
                     /locus_tag="QGF01_01540"
     CDS             5961..6752
                     /gene="tatC"
                     /locus_tag="QGF01_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HJN19615.1"
                     /translation="MSELNEIYSHLNELRTRVLRIVIIIGIIAIFLMAFHFESITYGG
                     IMFYYPSPEPLDNIAAQLTDYFRNNLVPEDVQLIQTAPGQAFFAQVYIAALVGLVAGT
                     PVIIREIVNFLKPALKENEINIGKTITMPAMGLFISGCLFAYFIVIPFMLDFLYKYGA
                     SAGLLTFLNIMDFVTFVLQFLLAFGLSFQLPMIMYALSQAGMTDTKFWRKNIRYAIII
                     IVVFGAVVTPDGSGVTMWFIAGPMIGLYLAGMILVERKDKQKLKT"
     gene            6802..6987
                     /locus_tag="QGF01_01545"
     CDS             6802..6987
                     /locus_tag="QGF01_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HJN19616.1"
                     /translation="MPMGTEWIWIAIIIGVFIFGAKKIPELAKTLGKAKGEYKKGEIE
                     GDKELKDFKENNNDKTS"
     gene            complement(6997..>7532)
                     /locus_tag="QGF01_01550"
     CDS             complement(6997..>7532)
                     /locus_tag="QGF01_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HJN19617.1"
                     /translation="STGVIYGIRILEKLKEFNIETHLIMSDWAQKCITMETEYDLNYV
                     KSLGTVISDDANMAASISSGTHKTDGMIVIPCSMKTLSSIANGYDETLVARAAGVIIK
                     ESRKLILVARETPLTAINLENMLKLARLGVIIMPPVTEFYTKPKNIDDLINHIVGKCL
                     DQFGIEHDLFPRWGSNN"
BASE COUNT         2478 a         1333 c         1135 g         2586 t
ORIGIN      
        1 ttaataaatt aaatatacta ttattattat agtagtaaaa aaaataatta cggtgatcct
       61 gattgattcc ataatgagtg acaatgtgat tcgagttgaa atgactagta tttgatgtat
      121 aattaatcaa tcctgctcag gaataaccga aagagtggta gtggttataa cattatttat
      181 ctgccttata tccttggtaa tgttgtgttc aatttttttt tctgattgat cctcaatttt
      241 gactagaata tcataaaatc caaaagttcc tttggcctct tttacaaaat ggaaactaag
      301 taattctttt aatattttca tctcatatcc aatcttacat tgtaccaaaa catatgcaac
      361 actcattgta cttcaccctt catttcttca cgtgttttaa aaacaggggt aattcccaat
      421 gacgtataat tacctgtggt acatgtaaaa cataatgaat ccttttgaat gccaatagca
      481 tttgcaagat tctcaggatc attatatcct agaaaatctg cccctatatc acgcctaaca
      541 ttttcaatga tttcttcttc tgaatatttc ttatcatcaa tataagttgc caattcatct
      601 tgagatggaa agtcaatacc tgcataacaa ggaaatttga ttggtgggta tgtaatgagc
      661 atagtaattt tacttgcacc tgcttttctt aatgctttta ttattgcctt tgaacttgtt
      721 cctctaacta ggctatcatc aacaacaaca acatgtctat tctcaataat ctctcgtatt
      781 ggaataatcc atctattgat ttcaatacgg tcagtttgat gtggttcaat aaaacttcta
      841 agtggaccct tcttgctata acgatctttt agtaatcctt catcaaaagg tactccaagt
      901 tcctgtgcaa atccaagtgc agcaggtcta gcagaatcag gtactggaat aactaaatca
      961 gcatctttta taggaaattt tctagcaagg tatttaccaa ttctttttct tgctacatag
     1021 atgttagttc cttccatttt actcgttgga tgtgcaaaat atgtaaactc gaatgaacaa
     1081 tgtgcatgag ttttatcctc tgaaaatgat tgtgattcta taccattttt agaaaatttg
     1141 attaattccc caggagaaac atcacgtatt aactcagctc ctatcgctga aaaagctgag
     1201 gattctgaag caacaatatg tactcttcca tcattttttt cacctaaaac aagtggtcga
     1261 aaaccgcatg gatcacgtgc tgcataaaca gatgtgtcat cagacaaaaa ggtaaagcaa
     1321 tacgagccaa tcatctcatt ctttagaatt gataatgctt tggacatttg ccctttttct
     1381 tttatcagtg aaaccaatct ttgtgctgca atcattgtat cacttgtatt ctgtggtgta
     1441 aatgaacaac caccaacaac attagatagt tcttctacgt ttgagattgt tccattatgt
     1501 gcaacacata aatcacgaac cttgagtggt tgtgcattat ctatattgct tttcccaatt
     1561 gtagaatatc taacatgccc aattgcagaa gcagatgaat actccatgga aattttcttg
     1621 aattctgagg ctgaagaaga aaccaaacct aattttttta atggtgattt atttggtaca
     1681 gcaattcccc atgcctcttg acctctatgt tgaagtgcac gcaatgaatc aataagcatt
     1741 ggaataacat taacaccgtc caaactatga atcccgacaa ctccacagtt ttctttaacc
     1801 atgtttcaac aaatcctcca acccattgaa atatcttttt cgcatgacat ctacattgct
     1861 cttcaccaga ttatttgatt tatgtgtaat actaatttga tcgccagaaa atttaccaag
     1921 aacaccaaag agtatttttt gcttagaaag atattgttta acgcgagtta tgttttttcc
     1981 atcaaccacc aacaaataac gagaatgact ttctgagaat aatatttttt catgactgag
     2041 atttttagta gatggtattt tttcgatgtc aatactacag cctatatttc cattaattga
     2101 caattctgat aatgctataa caaatcctcc ttttgagcaa tcatgtacac atttaatcaa
     2161 attatttttt atcaatgata gtactgtgat catgttgagt ttagaggttt taaaatcaac
     2221 agttggacag tttccaccaa taaatttatg aacgtattca aaatactcag aaccaccaag
     2281 ttcatctttt gttttaccaa caattaataa ataatcacca ttgcttggag gtaccggtac
     2341 caatggaggt ttatctataa ttcctagaac acctatgagt ggagttggtt ttattggtcc
     2401 actaggagtt tcattataga aacttacttt accaccaaca catggaactc tcaaaaattt
     2461 tgcaaaatca gacaagcctt caagtgcttc cataaaagtc caaaatattt ccggatcctc
     2521 aggattacca aattgaagat gatcaaccat tccgattgga tttgcgccag tacaaacaat
     2581 atttctacat gcttcttcaa aacagccaat agcaccctgc ctaggattaa gataacagtg
     2641 ttttgggttt ccatcaattt ttgctgaaag aaacttacca ttatccaatc taagaacaga
     2701 tgcatcaaaa cctggtttaa caacagttcg aattccaact tcatggtcat attgatgaaa
     2761 aacccaatgt ttacttgcaa tatttgggct tgataataat tttaaaattg ttttcgaata
     2821 atctaataac tttttatttt tattttcttt ttttagatgc ctcaaatact ttggaattgt
     2881 tttttttcta tcaagtaatg gtgcatgagc gacaaaatct gcaggaagta acgcaaatgt
     2941 attttcaccc tttctcacat gcatcatctt atcattttta acataaccaa tcagtgaaca
     3001 actcactcta aatttattac agatttcatt taattttggt aattttttct tctcggtgat
     3061 tattaacatt ctttcttgtg attcagaaat cattatttca tcaggtgata gaccagcttc
     3121 tctcgtgtga atattatcaa catctaactc tattccaatt cctaaactgt cggctgtttc
     3181 cgaaattgca catgataatc cgccacctcc caaatctttc attgcattta tgcatttttc
     3241 atttcttgcc tcaagtattg tttctataat taatttctca ataaatggat caggaatttg
     3301 tattgctgaa cgatcttcac tttctaatga atcagaagca aactgtgaac ctcctattcc
     3361 atcacggccg gtaggaccac ctattaaaac tactaaatct cccacattcg catgattttt
     3421 tatgagtttg tcttttttcc caaatccaat tgctgcaaca tcaactaatg cataattttt
     3481 ataacattca tcaaattcaa cttcaccacc tatagtcggt atacccaaac agtttccata
     3541 gtcagcaatt ccagaaacag cgtttttaaa aagccaacgt gcatgtgtat cattttcaat
     3601 atctccaaaa cgtaatccat caaaaattgc aataggacga gttccagttg ataaaatatc
     3661 acgaataaca ccgcctacac cagttgcagc tcctccatat ggttcaacag ctgatggatg
     3721 attatggctt tcaatatgta cagtcacaac ataaccgtct ccaacatcta aaacaccaga
     3781 atcatatcct ttttcattaa ttacatttct tccagtagta ggcaatattt tcagatgttt
     3841 tttggatgac ttgtacgaac aatgttctga ccactctgct gcaattatct gtcgttcggt
     3901 aatactaaga tctctaccaa tttttttggt aatatgtttt gattcttgaa cactcagact
     3961 catttttcaa taccagctgt ttttagtaat gattcaaaga tcagtgatga gggtttgtta
     4021 tctgttggat taatttctga ttcaactgct cgttctggat gaggcatcat cccaactaca
     4081 ttaccagatt cattacatac tccagcaata ttcaatgatg aaccattaag tgattcttca
     4141 taggtaaaaa ctatttgatt tttttcttgc aattctttca gtgttttatt atctgcaaaa
     4201 tatctacctt caccattagc tattgggatt gaaatttttt gtccttgttt tagcttattt
     4261 gtaaatggtg tattattatt ttgtactata agattaatcc atttgcacat aaaattcatt
     4321 gttgtgtttt ttaataaaac tcctggcaaa agaccagatt caacaagtat ttgaaaaccg
     4381 ttacatatac caagaactgg tacacctttt tttgccatac tcttcacatc cgaaatgatt
     4441 ggactgtgag cagcgatgac accagctctc aacctatcac catatgaaaa tccgcctggt
     4501 aggataacgg cgtcaatttt tgatggtaag tgtttttcat gccaaaaata ctctgcttct
     4561 agatcaaaaa catcagttag tacatgaaag atgtcacgat cgcaattact cccaggaaaa
     4621 actactatgc ctaccttcac gtattccctt gtaatttcgt gttattaaat caacacaaaa
     4681 cagtttttaa ttaaaaaatt gacaattgga catgggtcaa gtcagagata tcatggaaaa
     4741 aaatgttatc acaatcgaca taaatgaaac tgcgaataat gctgcaaatc aaatgaaaga
     4801 aaatgatatt agttttcttg taataattaa aaatggaaaa cctgttggtg ttgtaagtga
     4861 gcgtgacttc gttcaaaaac tatgtattaa caatcaaaac tcatccgatg taaaaatttc
     4921 agatataatg tcgtataaat ttagatgggt taaccccacc acaaaaattg aagatgcaat
     4981 tcaaaaaatg ctgaataata atattagaag actactggtt cttgatgatg aaaaacttgt
     5041 aggagttatt acacaaacag atctggcaag ttacttacgt gaaaagctaa tgattgacgg
     5101 tacgataaaa aatattaaaa aatagatcaa tcagtaataa tattaattga taatttactt
     5161 accattggat taaaaatcct taattcgtca caaagttgtt ctattttttt tttagccaat
     5221 tttttatttt tttcatttat ttgaaatttt aataatttag cagaattaat ttttgacact
     5281 gatgagaatt taccttgtaa aacaagatcg tttaaaattg tctcaccttc aggatcgctt
     5341 atgccggatt tattttctat tgttacatga acccaatatg taggcatatt cattttcata
     5401 attaaacgat aatatgttta acgcgaacgt atttgtatca gtaatgatga taatcgttta
     5461 aaatgagtcg ttctagatat tggaaattaa catctgaaga ggttgaaaat cttactcatg
     5521 atcctgataa aatcttaaac tgggaaatta aatgtataaa ggaaccagaa gtagatgcta
     5581 aatttattgg tgtatttctc tatcgacatg gtacaccata tgactatgac tcaaaaaaag
     5641 gaatagtcta ttactataat aatatagaac gaaatgatct accagaaatt acaaaatttc
     5701 ttcaaaaacg ttttggtggt gaaaaaattg aaaaaggtga aagaattttc ttggagggtt
     5761 ctaaagaaat ctatgcagga aaagaaattg cagatctagc taatgcatta aatgaaaaat
     5821 ttcatacaaa gtctgtcata tctatggaat tggacggtgc gactcaggat gaactgaaag
     5881 gatggggata tccagattct aaacttcttc ctattcctgg taaataatta atatgaaaaa
     5941 atctaacaga gatcccacag atgtctgaat taaatgaaat ttattcacat ttgaatgaac
     6001 tcagaacaag agtattacga atagtgatta taattggaat cattgccata tttctaatgg
     6061 cgtttcattt tgaaagtata acatatggtg gaatcatgtt ttattatcca tcccctgaac
     6121 ctctagataa tattgctgca caacttactg attattttag aaataatttg gtgccagaag
     6181 atgtacaact aattcaaact gcaccaggtc aagcattctt tgcacaagtg tatattgctg
     6241 cactagttgg tcttgttgct ggaacaccag taattattcg agaaattgtt aattttctga
     6301 aacccgcgtt aaaggaaaat gaaataaaca taggaaaaac tattaccatg ccagccatgg
     6361 gcctttttat ttctggttgc ctttttgcat atttcatagt tataccattc atgctagatt
     6421 tcttgtacaa atatggtgca tctgcaggtc ttctcacatt tttgaatata atggattttg
     6481 ttacatttgt tttacaattt ttattagcgt ttggattatc ctttcagtta ccaatgataa
     6541 tgtatgcact tagtcaagct ggtatgactg atacaaagtt ttggagaaaa aatatacgat
     6601 atgcaattat catcattgtg gtttttggtg cagtagtaac tcctgacggt agtggtgtga
     6661 caatgtggtt cattgcaggg ccaatgattg gattatatct agcaggcatg attcttgttg
     6721 aacgaaaaga taaacaaaaa ctgaagactt aaatctataa tttatgtatg aaaaataatg
     6781 tttgaatctc ttttacttca aatgcctatg ggaacagaat ggatatggat tgcaattatt
     6841 atcggtgtat tcatttttgg tgccaaaaaa attccagagc ttgcaaaaac gcttggcaaa
     6901 gcaaaaggtg aatataaaaa gggtgaaatt gaaggtgata aggaattaaa agattttaaa
     6961 gaaaacaata acgacaaaac atcctaacaa gttattttaa ttattagagc cccatctagg
     7021 aaaaagatca tgttcgatac caaattgatc taaacatttc ccaacaatat gatttatcaa
     7081 atcatcgata tttttgggtt tggtgtaaaa ctcagtaact ggtggcataa ttatgacccc
     7141 caatcttgct agttttagca tgttttctaa attaatagct gtcaaaggtg tttcccgtgc
     7201 tacaagaatt aattttcgtg attcttttat aatgacacca gctgcgcggg caactaatgt
     7261 ttcatcataa ccatttgcaa tactagataa tgttttcata ctgcatggaa taactatcat
     7321 gccgtcagtc ttatgtgtgc cacttgaaat gcttgcagcc atatttgcat catcagaaat
     7381 tactgtcccc aacgatttta cataatttag atcatactca gtttccatcg tgatacattt
     7441 ttgtgcccaa tctgacataa tcaaatgagt ctcgatattg aattctttca atttttcaag
     7501 aatcctaatt ccataaatta cacctgtact ac
//
LOCUS       DASCPJ010000029         4926 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000472608, whole genome shotgun sequence.
ACCESSION   DASCPJ010000029 DASCPJ010000000
VERSION     DASCPJ010000029.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000472608"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="QGF01_01555"
     CDS             complement(<1..514)
                     /locus_tag="QGF01_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HJN19618.1"
                     /translation="MKAAQIIEPNKPLKISEINTPSPTGKQVLIKVKAVGVCHSDLHL
                     WEGGYDTGDGFMKVTDRGVKFPVIPGHEIVGTVSATGDSVVGIKEGDDVLVYPWIGCG
                     TCPTCDKGDTNLCEAPQSLGVFQDGGYAEYSLIPDYKFLAQISGMDLEGAASLACSGL
                     TAYTAVKKAIL"
     gene            610..1320
                     /locus_tag="QGF01_01560"
     CDS             610..1320
                     /locus_tag="QGF01_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein ScpA"
                     /protein_id="HJN19619.1"
                     /translation="MDYDKQLTGISQEPVNILFNPSAISKKDIWQIDIIKILKILIKI
                     LEQTGKKDLKVAGMAALSSSLIYRMKVESIFALQKAAMEKKPLTSRNDVDIDILDIPY
                     RHESTYPVSLDELLDLLENLIGNIANPSSRRNNRLNFEAVEVPDFKDYFVSFENVVGK
                     YQDLIIRKIGDSGKGVLRQIVKDLETLDSIRCFFAILFLARDEKVTLEQKDDDILVTL
                     ITDIPKKSEGDGKSRKSG"
     gene            1295..1819
                     /locus_tag="QGF01_01565"
     CDS             1295..1819
                     /locus_tag="QGF01_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HJN19620.1"
                     /translation="MVKVENQDEITARLESALYSAGRPLSIEDLIKASGTESRIKTVS
                     ILDSIIKKTKSTFKAIEVVILPDGNYVFQLKPEYSSTVRKYASKPILAKATQKTLSYI
                     AYAQPISSKQLVETRGSGVYSHLKELRQLDFIEHQSIGRLKIYSTTDKFQKYFGIQGD
                     INTLKEKLFTRVRK"
     gene            1926..2303
                     /locus_tag="QGF01_01570"
     CDS             1926..2303
                     /locus_tag="QGF01_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HJN19621.1"
                     /translation="MKRSVENLSTSKITGGRRRPLRSRKKHETNRFPNEALVGEQITI
                     TRKVRGKNVKTAIKTIDTVNLVIDSKAKKVKILKVLENATNNDYQRRGIISKGAILET
                     AEGKCRVMSRPGQHGVVNAVLIK"
     gene            2308..2607
                     /locus_tag="QGF01_01575"
     CDS             2308..2607
                     /locus_tag="QGF01_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HJN19622.1"
                     /translation="MKDYDHIVVWLDYFNKTLSRKKGRRLPKDKCIFDPSLNELIESS
                     KAVGLEPTETNDQVRYPRRSYVRSGHIVLEKKYTKTEILNKIAVNLVAKRAKQSK"
     gene            2680..2958
                     /locus_tag="QGF01_01580"
     CDS             2680..2958
                     /locus_tag="QGF01_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HJN19623.1"
                     /translation="MQEVGEILHLASSGRVIVRLSKIASAGQILCDEKNVKVAKVVEL
                     IGPVAKPYASAALMTNNIKKFIGKKVFALDNPPAKKNKEHKRKKKWKR"
     gene            2946..3899
                     /locus_tag="QGF01_01585"
     CDS             2946..3899
                     /locus_tag="QGF01_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor IIB"
                     /protein_id="HJN19624.1"
                     /translation="METLEIQQCTTCNSSLVDDTQNGERICSGCGIVVVEQMEDYGPE
                     ARSGNMENKTNLARASGQTTYSQHDLGITTEISTESRDFSGKKINSEVASQMHNLRKW
                     QQRVRVSSSRDRRLTNILTKISNICQSCSLSENVLETASVIYRSLDGKNIEVKGKSVV
                     SISAAVVYMACKRCGVIRSLEEIIQGICQPKEVKPKTKLASKYYRTLVMELGTVTTPA
                     VTIDKYISKIANMTNTDVRVERLALEIAEKTKNRNLSDGKAPNGVAAAYLYLASILLG
                     QSVLQRDVSSISGVTEVTIRNRCKEILTNYKMNVTLRPSLA"
     gene            4001..4774
                     /gene="sufC"
                     /locus_tag="QGF01_01590"
     CDS             4001..4774
                     /gene="sufC"
                     /locus_tag="QGF01_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HJN19625.1"
                     /translation="MAVLEIKDLHVSRDGKEILKGVNMKTGPGEVHAIMGPNGSGKST
                     LAYTLLGHPKYQVTKGNILLDGESILDLKTDERAKKGLFLGFQYPTEVSGVGFSHFLR
                     TSYNALSKALQDNDREVFITVREFQKYLKENVQAVGMKDEFLSRYLNEGFSGGEKKRS
                     EVLQMTVLKPRVSILDEPDSGLDIDSVQAVAKAISNTFNKNTTTIMITHYARILNYLD
                     KLDFVHVFAQGRILKTGDSSLAGELEEKGYDWVLEKKSD"
     gene            4777..>4926
                     /locus_tag="QGF01_01595"
     CDS             4777..>4926
                     /locus_tag="QGF01_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase"
                     /protein_id="HJN19626.1"
                     /translation="MEVNDNEQYFFNFSFFKLDPKWRWMADLAKEESAKEVENVIRNS
                     GIKFRS"
BASE COUNT         1844 a          751 c          928 g         1403 t
ORIGIN      
        1 ttaaaattgc tttttttact gcagtgtaag cggtcaagcc agaacatgct aacgatgcag
       61 caccttctag atccattcct gaaatttgag ccaaaaattt gtaatctggt attaacgagt
      121 attcagcata tccaccatcc tgaaaaaccc ctaacgattg tggggcttcg cataaattgg
      181 tatcaccttt atcacaagtt ggacatgtac cacaacctat ccaaggatac accaatacat
      241 cgtcaccttc ctttattcct actacagaat ctccagttgc tgacactgtt ccaacaattt
      301 catggccagg aatgacagga aactttactc ctctatcagt aactttcatg aaaccatcac
      361 cagtgtcata tccaccctcc cagagatgta aatcactgtg gcagacaccc accgctttga
      421 ctttgattag aacttgctta cctgtaggtg atggcgtatt aatttcagat attttcaaag
      481 gtttgtttgg ctcgatgatt tgtgctgctt tcattgcgaa attttcagtt tcttataata
      541 taagagttga ctgaagagga gaaaaaaata gagtgtatag tattagtcaa tcataattta
      601 acaaattttg tggattacga taaacaactt actggtatat cacaggaacc agttaatatt
      661 ctctttaatc catctgcaat ctcaaaaaaa gatatctggc aaatagatat tatcaaaatt
      721 ttgaagatct tgataaaaat attagagcag actggaaaga aagatttgaa ggtagcaggt
      781 atggctgcac tttcttcttc tttgatttat agaatgaagg ttgaaagtat ttttgcgctg
      841 caaaaggccg ctatggagaa aaaaccatta acgagtagaa atgatgttga tattgatatt
      901 ttagacatac catataggca tgaatcaacg tatcccgtat cattagatga attattagat
      961 ctacttgaaa atttaattgg aaatatagca aatcctagta gtagaagaaa caatagactg
     1021 aattttgaag ctgtggaagt gccagatttt aaggattatt ttgtttcgtt tgaaaatgtg
     1081 gtaggcaaat atcaggattt gataattcgt aaaattggag attctggaaa aggtgtcttg
     1141 agacaaattg taaaagatct agaaacattg gactcgataa gatgcttttt tgcgatttta
     1201 ttcctagcac gtgatgaaaa agtgacatta gagcaaaaag atgatgatat tctggttact
     1261 ttaatcaccg atataccgaa aaaaagtgag ggagatggta aaagtagaaa atcaggatga
     1321 gataacagcc agacttgagt ctgcattata ctctgctgga agaccacttt cgatagaaga
     1381 tctaatcaaa gcatccggta cagaatcacg gataaagact gtatctatac tagattctat
     1441 aattaaaaaa accaaaagca cattcaaagc tatagaagtt gttatcttac ctgacggaaa
     1501 ttatgtattt caattaaaac cagaatatag ttccacggtt agaaaatatg catcaaagcc
     1561 catcttagca aaagctacac aaaaaacatt atcatatata gcatatgcac aacctattag
     1621 ctcaaagcaa cttgtggaaa ccagaggttc tggtgtttat tcacatttaa aagaactacg
     1681 acaactggat ttcattgaac atcaaagtat tggcagacta aaaatttatt cgacaactga
     1741 taaattccaa aaatattttg gtatacaggg agatatcaat actctaaaag aaaaattatt
     1801 cacgagagta agaaaataaa ccaaaatcat aatttgtgga taaaatcatc aaaaaatatg
     1861 aaaacacgca aaaatctgta atgatttttc ggtttgtata aattagagtg aattaaaagg
     1921 catttatgaa aaggtcagtt gagaatctat caacaagtaa aataacaggt ggtcgtcgac
     1981 gtccattacg ttcaagaaaa aaacatgaaa ctaataggtt tcctaatgag gcattagttg
     2041 gagaacagat cacaataact agaaaggttc gtggaaagaa tgtgaaaact gccataaaaa
     2101 caattgacac tgttaatttg gtaattgatt caaaagcaaa aaaagtaaaa attctaaaag
     2161 ttttggaaaa tgcaacaaat aacgattatc aacgacgtgg aattatttct aaaggtgcaa
     2221 tacttgaaac agcagaagga aaatgtagag ttatgtcaag acctggacaa catggtgttg
     2281 ttaacgcagt tttaataaag tgatttcatg aaagattatg accatatcgt ggtctggcta
     2341 gattatttta ataagacatt atcaagaaaa aaaggaagaa gattaccaaa agataaatgc
     2401 atttttgatc cttcattaaa tgaattaatt gagtcatcaa aagcagttgg gcttgaacca
     2461 actgaaacaa atgatcaagt tagataccca agaagatcat atgttaggtc aggacatata
     2521 gtattagaaa aaaaatatac aaagactgaa attttaaata aaattgcagt gaatctggta
     2581 gcaaaaagag caaagcagtc aaaataaaat tctatctagc tttaagctta agtaaaattg
     2641 acagaacgtt tgatcaacta tcaaacgtta ttggaataat tgcaggaggt aggggaaatt
     2701 ttgcatcttg ccagtagtgg cagagtcata gtaagacttt cgaagatagc gagcgcaggc
     2761 caaatacttt gtgatgaaaa gaatgtcaaa gttgcaaaag ttgtagaatt aattgggcct
     2821 gtcgcaaagc cgtatgcatc tgcagcttta atgaccaata acataaaaaa atttattggg
     2881 aaaaaagtat ttgcactcga taatcctcct gcgaaaaaaa ataaagaaca caagaggaaa
     2941 aaaaaatgga aacgttagaa attcaacaat gtacaacgtg caattcatca ctagttgatg
     3001 atacacaaaa tggcgaaagg atttgttctg gctgcggaat agtagtagta gaacaaatgg
     3061 aagattacgg accagaagca agaagtggta acatggaaaa taaaacaaat cttgcaaggg
     3121 catctggaca aacaacatat tctcaacatg atttgggaat cactacagaa atttccactg
     3181 aatctagaga ttttagtggg aaaaaaatca attcagaagt agcatcacaa atgcacaatc
     3241 tgagaaaatg gcagcaaaga gtcagagttt ctagttcaag agaccgaaga ctgactaata
     3301 ttttgacaaa aatttcaaat atttgccaaa gctgctctct ttcagaaaat gttttggaaa
     3361 ccgcctcggt gatttacaga agtcttgatg gtaaaaatat cgaagtgaag ggaaaatctg
     3421 tagttagtat atctgcagca gtagtttata tggcatgcaa gcgatgtggc gtgattcgtt
     3481 ccttagaaga aataattcaa ggaatttgtc aaccaaaaga agttaaacca aagacaaagt
     3541 tagcttcgaa atattacaga actttggtaa tggaacttgg aactgttaca acaccagctg
     3601 taacaattga taagtacatt tcaaaaattg caaacatgac aaacactgat gtgagagttg
     3661 aaagattagc tcttgaaatt gcagagaaaa ccaaaaatcg taatctttct gatggaaaag
     3721 cgcctaatgg agtcgctgca gcatatctat atctagcatc aattcttttg ggacagagcg
     3781 ttcttcaaag agacgtatct agtatctctg gtgtaactga ggttacaatt agaaaccgat
     3841 gtaaggagat tctaactaat tacaaaatga atgtgacact tagaccatca ttagcctaaa
     3901 cccctttttt ctttttattt cgtctaaaga attcctattt tttgttagga gtagctaaaa
     3961 ttcgtctcgg ttatatatag aaattaagtc tattgaccat atggcagtgc tagaaatcaa
     4021 agatcttcat gtttccagag atggtaagga aattctcaag ggcgtgaata tgaaaactgg
     4081 acctggtgaa gtacatgcaa taatgggccc taacggttca ggaaagagta ctctagcata
     4141 tacattactc ggtcatccaa aatatcaagt aactaaagga aatatcttgt tagatggaga
     4201 aagtatttta gatttaaaaa ctgatgaaag agcaaaaaaa ggactgtttt taggttttca
     4261 atacccaact gaagtttctg gtgttggttt ttctcatttt cttagaacat catacaatgc
     4321 attaagtaaa gcacttcaag ataatgatcg tgaagtcttc atcacagtta gggaatttca
     4381 aaaatacctc aaggaaaatg tccaagcggt tggaatgaaa gatgaatttc tatcaagata
     4441 tctcaatgaa ggattctctg gtggtgaaaa aaagagatcc gaagtattgc aaatgactgt
     4501 gttgaagcca agagtttcta tattagatga acctgattca ggtttggata tagattcagt
     4561 tcaagcagtt gctaaagcaa tcagcaacac atttaacaaa aatacaacca caatcatgat
     4621 tacacattat gcaagaattc ttaactatct agacaaactt gattttgttc atgtttttgc
     4681 tcaaggaaga atactaaaaa ctggtgactc gtctctcgcg ggtgaacttg aagaaaaagg
     4741 ttatgactgg gtgttagaaa agaaatctga ctaagattgg aagtaaacga taacgaacag
     4801 tattttttta atttttcatt tttcaaactt gatccaaagt ggagatggat ggctgattta
     4861 gccaaggaag aatctgctaa agaagttgaa aatgtgatta gaaattcggg tataaaattc
     4921 agatca
//
LOCUS       DASCPJ010000030         3057 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000584834, whole genome shotgun sequence.
ACCESSION   DASCPJ010000030 DASCPJ010000000
VERSION     DASCPJ010000030.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000584834"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /gene="rqcH"
                     /locus_tag="QGF01_01600"
     CDS             complement(<1..1870)
                     /gene="rqcH"
                     /locus_tag="QGF01_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HJN19627.1"
                     /translation="MQLAGIELRYLVNDIGQKTEDYYVANIYGISKDSLLFKFRHPEN
                     PDVLLMLSTSGIWVTKVKIDQIEPNKLLRRLRSDLLRSKLKEIKQIGTERVVYLTFSN
                     FEKEFVIVSELFADGNVILCNSDMKILALLYSVNVRHRQLRVGSPYVPPPQNNFDILK
                     MTEADLGEVRFTSTPVAKWIGRTLGLPRKYVEEITRLSNVESKKSGTEISNEEIKSLF
                     DNATKVINNVVNGQHDPVISRDEEINVHPIPLGEKNLEKISSFMEGLDTAFTENILNK
                     GKTTQSSSFQKKISELETRLNEQTNAIKIVTDKSEKIAGVANSLFDYVSQGISSMSDS
                     RIDGLLKTNNAEIIKEKGITHIKVEDAKIRVDLESSLPTIASKLFNESKKQKSAIGAI
                     QKLIKKTEKELEKTIEEGENAKQVISYSEVRKKNWFERYRWFFTSDGFLVVGGRDSSS
                     NSAVIRKHLEKDDKVFHGEIYGSPFFLLKDDGSSMTSSLNEVAHATVCFSRAWKESMY
                     GTNAYWVNPDQVKKGAPSGQSMGKGSFIIEGQRNFVKISTLKLCVAIVEYEGTHLLTC
                     GPPSLKENCVAYVMIEPTGMEMVDVAKKIRFELISMNETIAKSFSVDDYVRVLPSGP"
     gene            1952..2710
                     /locus_tag="QGF01_01605"
     CDS             1952..2710
                     /locus_tag="QGF01_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19628.1"
                     /translation="MNLEISLVRHGKKKRRFGSRERKPLDFVDPDRDPHTYIERYVRE
                     EDYRDWFDRNYPDYTIYEAVGLTESDLDEVERRFAPAHEETSLEPEPEPDGSVSGKWT
                     AEPSKEERPRVAKRRDSPPTERRDSSPTEPVDASTPPVSSQEELLTKKRTNVLFWMRI
                     AFALIGGAIATFAFDSIEDSEEKRWTSIIFMIGLFIATCVIAKGMKINFSRADRKKLV
                     TTGIGSYIFLYLFAWILSYTLLNLPQSNIPTPFT"
     gene            2729..>3057
                     /gene="cbiT"
                     /locus_tag="QGF01_01610"
     CDS             2729..>3057
                     /gene="cbiT"
                     /locus_tag="QGF01_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HJN19629.1"
                     /translation="MWNAKTPGIPDELFDRDENVPITKEEVRVVQISKARLKPGMIVY
                     DIGCGSGSISVEAAHQVEESGHVHAVDYDAKAVELTKKNLEKFNLANVSVILGNAKEK
                     ISELPNA"
BASE COUNT          938 a          595 c          539 g          985 t
ORIGIN      
        1 ttggaccaga tggtagcacc cgcacataat catcaacact gaatgatttc gcaatagtct
       61 cattcattga tataagctca aaacgaattt tctttgctac atcaaccatt tccattcccg
      121 ttggctctat cataacatag gcaacacagt tttccttcag ggatggcggt ccacaagtca
      181 gcaaatgagt tccttcatac tctacaatgg cgacacacag tttcagagta gaaattttga
      241 caaagttacg ttgtccttcg atgataaacg aaccttttcc catggattgt ccgcttgggg
      301 ctcctttttt gacttggtct gggttaaccc aataagcatt ggtaccatac attgattctt
      361 tccaggcacg gctaaaacaa actgtagcat gtgcaacttc attcaggctt gaagtcatgg
      421 aagaaccatc atctttcagc aaaaagaacg gagaaccata aatttcgccg tgaaagacct
      481 tgtcgtcttt ttcaagatgt tttcgtatta cagcagaatt ggatgatgaa tcacgcccac
      541 ccacgaccaa aaatccgtca gatgtaaaaa accatctata tctttcaaac caatttttct
      601 ttctaacctc agagtagcta attacctgct ttgcattttc accttcctca atagtttttt
      661 ctaactcctt ctcagttttt ttaattaatt tctgaattgc acctattgca gatttttgtt
      721 tctttgattc gttgaatagt ttggatgcaa ttgttggaag agacgattcc aaatctactc
      781 tgattttagc atcttcaact ttgatatgtg tgattccttt ttcttttata atttctgcat
      841 tgttagtttt caataaacca tcaattcgcg aatcactcat agatgaaatt ccctgagaca
      901 cataatcaaa aagtgagttt gcaacaccag caattttttc tgatttgtca gttacaatct
      961 ttattgcatt tgtttgttca ttcaatcttg tttctaactc tgaaattttt ttctgaaaag
     1021 aactgctttg tgttgttttt cctttattaa gaatattttc cgtgaaagca gtatcaagac
     1081 cctccatgaa actagaaatt ttttcaagat ttttctctcc caatgggata ggatgtacgt
     1141 ttatctcttc atcacgagaa attacgggat cgtgttggcc attcaccaca ttgttgataa
     1201 ctttagtggc attatcaaag agactcttta tctcttcgtt tgatatttct gtaccactct
     1261 tttttgattc aacatttgac aatcttgtta tctcttcaac atattttctt ggcagaccta
     1321 acgtacgtcc tatccatttt gcaactggag ttgaagtaaa tctaacttca ccaagatcag
     1381 cttctgtcat ttttagtata tcgaagttat tttgtggagg agggacatat ggtgatccca
     1441 ctcttaattg tctatgtcta acattaacag aataaagcaa cgcaagaatt ttcatgtcac
     1501 tattgcaaag tatgacattt ccatccgcaa ataattcact tacgattaca aactcttttt
     1561 caaaattaga aaatgttaga tatacaacac gttcagttcc tatttgtttt atctccttca
     1621 gtttagatct tagaagatca cttcggaggc gtcttagcag tttgtttggt tctatttgat
     1681 ctatctttac ttttgtaacc caaattccac tagttgataa catcagtaaa acatcaggat
     1741 tttccgggtg gcgaaacttg aaaagtagac tatcctttga aataccatag atattggcga
     1801 cgtaataatc ctcagttttc tgacctatgt cattaactag atatcttagt tcaattccag
     1861 ctagttgcat aagtgggtat atgtatctcc gattatactc ttatcgattt tccagcctac
     1921 tagacaaagg ttttaaaaaa ggcacatctg attgaatcta gaaatttcat tggttagaca
     1981 cggtaaaaag aaaagaagat ttggttcaag agaaaggaag cctcttgatt ttgtagatcc
     2041 agacagagat cctcatacct atatcgagag atatgttaga gaagaagact atagggattg
     2101 gtttgataga aattatcctg attataccat atacgaagca gtaggattaa cagaatctga
     2161 tttggatgag gtagagcgaa gatttgctcc tgctcacgaa gaaacttcct tagaacctga
     2221 accagaacca gatggttctg tgtcaggaaa gtggacagct gaacccagta aagaagaaag
     2281 accacgagta gctaaacgac gtgattcacc accaactgaa cgacgtgact catcaccaac
     2341 tgaaccagtg gatgcatcta cgccacctgt tagttcgcaa gaagaacttc taacgaaaaa
     2401 acgtaccaat gtactttttt ggatgcgtat tgcgtttgca cttataggcg gagctatagc
     2461 tacatttgct tttgattcca tagaggacag tgaagaaaaa agatggacct caatcatatt
     2521 catgataggg ttgtttattg caacttgtgt tatcgcaaag ggaatgaaga taaacttctc
     2581 aagggcggat agaaaaaaac ttgtgacaac aggaatcgga agttacatct ttctttatct
     2641 atttgcatgg atcttaagct acacactgtt gaatcttcca caaagcaaca taccaactcc
     2701 atttacctaa ccaacttgat gtatcattat gtggaatgca aaaactcctg gcatccctga
     2761 cgaacttttc gatagggatg aaaatgttcc catcacaaag gaagaagtca gagtagttca
     2821 gattagcaag gctagactaa aacctggaat gatagtttac gatattgggt gtggctctgg
     2881 ttctatatca gtagaggctg cacatcaagt tgaggaatct ggtcacgtac atgctgtaga
     2941 ttatgatgca aaagcagtcg agctcactaa aaaaaatctt gagaaattta atcttgcaaa
     3001 tgtttcagtc attctcggaa acgctaaaga aaaaatttca gaactaccaa atgcaga
//
LOCUS       DASCPJ010000031         2196 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000595798, whole genome shotgun sequence.
ACCESSION   DASCPJ010000031 DASCPJ010000000
VERSION     DASCPJ010000031.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000595798"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(184..477)
                     /locus_tag="QGF01_01615"
     CDS             complement(184..477)
                     /locus_tag="QGF01_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19630.1"
                     /translation="MGFFSKYKEKKIAELKEKQSMMNGKELKKLLTIMKKDRDKIERQ
                     TGKRPEIDDTTRLFMQKILNVWMSEGKDIDDEKFWNAVDYNEQFDHPVEYYER"
     gene            complement(515..706)
                     /locus_tag="QGF01_01620"
     CDS             complement(515..706)
                     /locus_tag="QGF01_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2-type zinc finger protein"
                     /protein_id="HJN19631.1"
                     /translation="MGLFKKIQCDKCDEKFRKQEELMHHQLLAHAEDSRYDCKVCDEY
                     FDSMEAMRAHLMRKHSYKR"
     gene            complement(715..981)
                     /locus_tag="QGF01_01625"
     CDS             complement(715..981)
                     /locus_tag="QGF01_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19632.1"
                     /translation="MEDPTGGMWIYLIFFMIPLVRILPRMIKKYKRKNAGLEPEPEPA
                     EKYNEKQFFTREQMSEPPRYTEEPQPEPEPEPDSKGGWMKKDDF"
     gene            1268..1681
                     /locus_tag="QGF01_01630"
     CDS             1268..1681
                     /locus_tag="QGF01_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HJN19633.1"
                     /translation="MDAYLSQHKLHDVVNMGLRAAVGVVFIVHGSGKFNPGFLGFMEM
                     LGLPPEMQIPIALAETVPGILLLIGVFTRLSASLLSIVMLGAIFYVKQAGSLTGERGF
                     ELDLILLAACLVVMIAGPGRISIAHVVKKLPRCLH"
     gene            1730..>2196
                     /locus_tag="QGF01_01635"
     CDS             1730..>2196
                     /locus_tag="QGF01_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19634.1"
                     /translation="MNRSSKITIAAILVIGVFSISIMTTPAFAEWDKTNYPKIQGTIP
                     ADNSEQKDTIALSVAMAVGENSVSDGKAVYGKLTDVNEFLVYKVVVITDDHAYYKILV
                     DAGNGEALYVSDMIKKGDHKKISDKKHSDKAKKYFKGMSDEQIAEKKRQFKEMG"
BASE COUNT          651 a          400 c          414 g          731 t
ORIGIN      
        1 tcattgccgt aatgccgatc acaagatcaa atatagacta actgacccag aaaaagttag
       61 ggctattgtg cttgactacg gcaaaacatc tcttcagaat tctgtagaca atttatctga
      121 tgtctttttg tcactcaaat aattcttgat ctaaacatac aaaatttggg cacagtacaa
      181 cagctatctt tcgtaatatt caacaggatg atcaaattgc tcgttatagt caaccgcatt
      241 ccaaaacttt tcatcgtcaa tgtctttgcc ctcagacatc caaacgttga gaattttctg
      301 catgaataat cttgtagtgt catctatttc cggtctctta ccagtttgcc tctctatttt
      361 gtctcggtcc ttcttcatga tggttagaag tttctttagc tctttaccat tcatcatgga
      421 ttgtttttct ttcaattcag caattttctt ttctttgtat tttgagaaaa aacccatacc
      481 aatcaattaa cattcaatgt cttaaaattt tatgctatct tttgtaagaa tgctttctca
      541 ttagatgtgc ccgcattgct tccatcgaat caaaatattc atcgcaaacc ttacaatcat
      601 accgtgaatc ctcagcgtgc gctaaaagtt gatgatgcat taattcttcc tgttttcgaa
      661 atttttcatc acatttgtca cattgaattt ttttgaacaa acccatgttt ttcactaaaa
      721 atcatctttt ttcatccagc cgcctttact gtctggttca ggttccggtt ctggctgagg
      781 ttcttccgtg tatcttggtg gttccgacat ttgctctctg gtgaaaaatt gtttctcatt
      841 gtatttttct gctggttccg gttctggctc caatccagcg tttttgcgct tatatttttt
      901 tatcatgcgt ggaagtattc ttaccaacgg aatcataaag aaaatcaaat agatccacat
      961 tccaccagta gggtcttcca tgatttgata taatgaacag cctatttcat tttttcgact
     1021 gttttacgta ctagattatt ttcgagctgt atcaaaagca gagaacgtgc ggctgattta
     1081 ttaaatcccg caaaaagatt tggtcgtagg ctagggatga aaaatgtgtg aacataaaaa
     1141 aattataaaa aacaaaaaca tatgggcatg ctcagaatgt gacgaggaat ttctgataga
     1201 gtaatctgaa taattagttt tgatatctat accaccaact tattaaattc tgcagaaaag
     1261 cctctttatg gacgcatatc tgagccaaca caaactgcat gatgttgtga acatgggttt
     1321 gcgtgcggct gttggtgtgg tttttatcgt tcacggttct ggcaaattta atcctggatt
     1381 tcttgggttt atggaaatgc tcggactacc tccagagatg caaattccaa tcgcacttgc
     1441 agaaactgtt ccaggaattt tgttgttgat aggagttttt acaagacttt cagcgtcatt
     1501 gctttcgata gttatgctag gtgcaatttt ctatgtcaag caggcgggca gtttgactgg
     1561 tgaacgtggt tttgagttag atttgatatt acttgctgca tgtttggttg taatgattgc
     1621 tggaccagga aggatttcta tagcacatgt agtaaagaag ctgccacgat gtttgcacta
     1681 ggaacaaatt gtaggatcat tttttataga gtgcaaaaag tcataatcca tgaatcggtc
     1741 atctaagatt acaatcgcag cgattctggt aattggcgta ttttcaatat caatcatgac
     1801 tactcctgca tttgcagaat gggataagac caattatcca aaaattcagg gaaccattcc
     1861 agctgataac tcggaacaaa aggatacaat cgcactgtct gttgcaatgg cggttggtga
     1921 aaattctgta tcagatggaa aggcagttta tggaaaactg acagatgtca atgaatttct
     1981 ggtttacaag gttgtagtaa tcactgacga tcatgcatat tacaaaattc tagttgacgc
     2041 aggaaatggt gaggcacttt atgtatctga tatgataaaa aagggtgacc acaaaaaaat
     2101 ctcagataaa aaacatagtg acaaagctaa aaaatatttc aagggaatgt ctgatgaaca
     2161 gatagcagaa aagaaacgtc aattcaaaga gatggg
//
LOCUS       DASCPJ010000032        11150 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000604653, whole genome shotgun sequence.
ACCESSION   DASCPJ010000032 DASCPJ010000000
VERSION     DASCPJ010000032.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000604653"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="QGF01_01640"
     CDS             <1..466
                     /locus_tag="QGF01_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HJN19635.1"
                     /translation="FKNRDFFLFPKPHVLASATLEEVQQCGLGYRSSYIIDASRAVLS
                     EKINFNFLKKTDYQTAKESLVKIPGIGNKVADCIMLYSLEKLEAFPLDVWMLKILEKY
                     YSDEFDIDTKTITPRKYDMIHQKAVDYFGSHAGYAQQFLFKMERDLNGKKWL"
     gene            500..574
                     /locus_tag="QGF01_01645"
     tRNA            500..574
                     /locus_tag="QGF01_01645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Arg,seq:tct)
     gene            621..971
                     /locus_tag="QGF01_01650"
     CDS             621..971
                     /locus_tag="QGF01_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19636.1"
                     /translation="MKDIELRLDSDDVKSYSEIKGFVVVHYGGKYDGVVINTQIIGSN
                     NLIVYKSFNGKQISQNMSRLFISKDHMSDNKAEFTAMIEFEPEHPHDVKFRASIIEQH
                     KEVESDQLFAKLTV"
     gene            complement(968..1447)
                     /locus_tag="QGF01_01655"
     CDS             complement(968..1447)
                     /locus_tag="QGF01_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HJN19637.1"
                     /translation="MSTVSSGHQYGVGLLAVLVAASVGIGYYQMYYLPEQLATPDVDE
                     HVLHPDKSTHIEMIVGSADPDQQDNYVPKLVNVQLTIDNHVIWTNVDDVPHTVTPDHR
                     YTDGYSGDFGSSGVVKAGEIYEFLFTEAPPNIAVEIKYHCDPHPWMTGTLLIGQARF"
     gene            complement(1444..2895)
                     /locus_tag="QGF01_01660"
     CDS             complement(1444..2895)
                     /locus_tag="QGF01_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HJN19638.1"
                     /translation="MAVSLERRNSTANFLYWIWNELDRTIFTAIKFSFPSRFVSPFGF
                     LGMLTFIMFVTLGLTGALLMFYYEPIMTRSWDSVQFINDSVPFGFHIRNLHYHGSNAM
                     VMLAILHMYYQYFSGRYKIRNEILWVTGVILGTVTILEAFTGYDVIFSERAELAISIA
                     ASLTNSIPVAGPMIRELAFGAGFHDFVLRFYAQHVFILPLVMLGLMAVHFPRFLVFDV
                     PMVMAICGALCLAGGVFPVDLGFKFEPTVPPGITVPEWYLTGLYAFLRTQYDKFLTGV
                     LWPGLFILALLVTPFIDRYKKFSWKDRPWITAMGITGIAQILVTTYWGFYIPPDTTIP
                     LVERLVIDPINLYLVMILLVPLSFGFTLMMIHLAKDAERKAKLAKESAPHKVSPINLS
                     IKWTNWLLVALLAFQVFLNIAAYNAFLSGMNNVNLWLTGVILITFAGFFHLYRYGMAQ
                     SKQIPKPPSISSTKAVESTQKSLPPTNESEESS"
     gene            complement(2879..3481)
                     /locus_tag="QGF01_01665"
     CDS             complement(2879..3481)
                     /locus_tag="QGF01_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HJN19639.1"
                     /translation="MSEQEKGPGGMSRRDFLKLLGAAGTSMAFAPFVPWGKFLPNPSS
                     STLSRVPVILPDGTQANLNTYPINHAEVITYPETSDEVLNEEAFRKWQFIRLPEKFGG
                     GRKNVSAFRAYSMVCLHLWCLWKYWPEEGRTRGECPCHGSMYDVRTGTSYLGPASLQA
                     PPSNTLAKLDFEADSDGFMFISPPIWGVNKNGVVGYGRFA"
     gene            complement(3507..7787)
                     /locus_tag="QGF01_01670"
     CDS             complement(3507..7787)
                     /locus_tag="QGF01_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HJN19640.1"
                     /translation="MFKTIIFFTLFFLTCILVQEESYGFIESFEPSQTPNFNFDKTSD
                     IISLDITNVNDQPKRYLVFGYDSLNNAYQDTKNIVYGINSNSGFFSVGILDESEALNL
                     KSKGYYVIEDYMLDFHSKYLSTNAATDISQIGNIAESEKVHTLYDVTGDGVTIAVVDT
                     GVDFSNPDITDSLLRNHLNEPVMLDADGQGIILTNSTFYANKSEFGLIKNYTKSHTIP
                     EDATSTVYLTNEGVFLDMIQNGNNTKISVYNSFYPYAGIGTVLAGTVEHDFKIGNTER
                     VYIKSQSGIYHLGYILNAHMGKVQVVPVLVTDPNEAGIYDTITPDLSTSWMDFFRDEP
                     GVRSADYDFDFTDETSISIGDGNELLIYDSDGDGKNDYSAGIIGASVVDVYGLFSKKA
                     EIDDNVGAVNGTLLPPMDKDGRFFGLMTDFFGHGTASSSTIASKGITEYDIYNDTKKY
                     TIKGIAPDVKILPVKALWAGDVVYAWMWIAGFENEDNSWVYTGEPKANIISNSWGIST
                     FPNLGYGPGFDFLAHIVNILATPESLHENYPGMTIVSSAGNSGHGYGTIASPGISSYG
                     ISVGAVTSNDFVGYSSFKEQPRFGNTTAHSDHVVDFSSRGPGILGEPKPDLMSIGAYS
                     FVPSAVTITPDTDEEQFSLFGGTSMSAPITAGATALVIESLNERQIDYDPFAIRNILM
                     STTDDLQNDPFTQGTGLVNALSAVKVVNGEGGKFFVHNNESFSNIKKVIDRPISLFDL
                     EIFGLEKAKVSDNTFPITSWYGGTLLPGEKTTTIYTLENPTNKSLDIKINPVTLKLIE
                     KLQMDGVTKVHLQDPILNDTGVYRPNYIKLSDIGKDEPSDQTSIYKQPNQDSIINPDS
                     SLMVLNLNFPFSTFMNQTDSIYADDLKISSLYVYDWRDKNKDNEISSDELSLVNRGGS
                     WGTVQEIRVSNPVEKFEDEPVIGVYPVPERISYWIGNTNQNSTAMNYTLSASYYKNDL
                     WEDVVVDHQEITIPPNNSTKISATISPPAEKHPGIYQGFLNFEGEHHNVRSPVSYNIL
                     KVVDQKEKQIVIDGLEGNTLYGNGYVKGAFDMSGKYMAGDWRQYSFDIQDRTINSASI
                     NLGWENDDTNFSAFMIDPQGKIIQTNVPSGVFGHFWSWPTIDWLGVTPFSQGGGFFPV
                     KNKDNTSTVMFAPINQTGTYTLLLHSTLFNGESITEPFSLAVKFDTILADDDPPEIIF
                     DMPEFINNNFKIRPVIIEENLNFVKFYVDGNEVESETPVCNECNMNWKLNDGPHELRI
                     HASDILENDADKTFSFIVDNTPPKITLNLPKYNTTVSNSLKIDFKIDDKNLSETGAIQ
                     ILLPQGDSYEDITSLSYNTTNVFAGTYDLKIVAKDLAGNEAVETVPFIVDHSFIQKSM
                     ENVKPEIQEPNMLIIVISIVIITIIVAFVAIKKARSKHDQDKTLKQDL"
     gene            complement(7818..8825)
                     /locus_tag="QGF01_01675"
     CDS             complement(7818..8825)
                     /locus_tag="QGF01_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HJN19641.1"
                     /translation="MIKNHQIQLKLLGLATVFVLIVFTLPQAFADELTLFTNQQIYTE
                     RHPLYVYGDAPAGQPLVVRLFSPDGSITEFQQVSTNNDGTYGVKLLEWPSSSTTFPFG
                     TYTVESITLDGTSVKIDVKFASTTEVTKIPIERDLNVQVFAPEIAATHKSFRIYVQVT
                     SDGLMVNDEAASLLSSSHLHSPDGKVQSMIKSLSMLHEGLFFVEHTPTLEGTYIFHMV
                     AFNQGTQSHGSAATLVLGQDIAGLSRQVVTLNEVITTASDEVGTLQTEIHGFGDTFEG
                     ASDKIRGSLTEIDNSVSSMSKAVTTIEEASLQVNSLLFPIVASIAVILALQITILARR
                     R"
     gene            complement(8904..>11150)
                     /locus_tag="QGF01_01680"
     CDS             complement(8904..>11150)
                     /locus_tag="QGF01_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19642.1"
                     /translation="YSQEREVGTTANAGDTFAVSYNDTITSIANLKLVETGDDTGVFV
                     GQFLTSSGNIGNDLKFTYYDNKDASGTTVDVYTSVKIATESGSISFDRQVYPVPFFNG
                     WLTDGSGDTLDDPFDTSPDPTAGATHGNVTVYFTVSDGDEVGQTITSAADRVYIKLNG
                     NVIATGGAAAADLTSGETGPLTEVERGTSVYEGSFSLDHQETIYDGVTGTCATSTCAA
                     FNVTSGMVLQAAYDDQTDDAGVNTVFYDSATVDLRTGSISTDKEVYVIGSDIIVTITD
                     PDLNLDSDSSESYSTELVEWDSSASSSRLMSNSEFAAMPSKFIETGSNTGVFQTVFTF
                     PSSISSTSIEQGEEVTITYRDQGLSGKDEVHDDYNDIEATIYASNFGAIVELDRAIYD
                     WTDRVLITVTAPDHNQDSEKIENIGTTALPIKVSTRQETFPTGSTSYTLRETGEDTGV
                     YYGEYVLKGFADGNVFETALTADSATGNTDGVINTAGQTDGITVSYEYTDGSVSLASA
                     LIAWNIGEISFGDSSVSPGGSSTITLVDGDLDKNPDVIDTKTANVFSDSDSGGISITL
                     HETGEATGVFETTVFFTADDKSTGSLLRVSEGDTVTVEYTDQTLPEPYESADTLTFAA
                     TTTVGTVYPPLERAPASNARVVDAFGSQVSEVSADQQVQIAADVSNGTGKDQSFAYLV
                     QVQDANGVTVSLAWITGALTSGQSMSPALSWTPSASGSYTATVFVWQSVDNPTALSPT
                     VSVNIDVV"
BASE COUNT         3366 a         2156 c         2033 g         3595 t
ORIGIN      
        1 attcaaaaat agggattttt ttttatttcc taaacctcac gttctggcaa gtgctacatt
       61 agaagaagta cagcagtgtg gattaggata cagatcatca tacataatag atgcatctcg
      121 cgctgttcta tcagaaaaaa ttaattttaa ttttctaaaa aaaactgatt atcagacagc
      181 aaaagaatca cttgtaaaaa ttcctgggat agggaataaa gttgctgact gtataatgtt
      241 atattcactt gaaaaattag aagcatttcc actagatgtc tggatgttaa aaattctaga
      301 aaaatattat tcagatgaat ttgatattga cacaaaaact attacaccaa ggaaatatga
      361 tatgatccat caaaaggctg tagattattt tgggagtcat gcaggttatg cacagcaatt
      421 tctcttcaaa atggaaaggg atttgaatgg aaaaaagtgg ttgtaaaacc cttaatagag
      481 taaaaaattt ttgattcttg ggctcatagc tcagcttgga tagagtgttg gacttctaat
      541 ccaatggtca agggatcgaa gccctttggg cccattattt tttaacaaaa ttatttattc
      601 tcataaatga ttcaatcatt atgaaagata tagaattacg attagatagt gatgatgtaa
      661 aatcatattc tgagatcaaa ggatttgttg tagtacatta tggaggaaaa tatgatggtg
      721 tggtaattaa tactcagatc ataggttcta acaatctaat tgtttacaaa tcatttaatg
      781 gaaaacaaat ttctcaaaat atgtcacgat tattcataag caaagatcat atgagtgata
      841 ataaagcaga gtttactgcc atgatcgagt ttgaacctga gcatccacat gatgttaagt
      901 ttcgtgcatc aattatagaa caacataaag aagtagaaag tgaccagtta tttgcaaaat
      961 taacagtcta gaatctagcc tgtcctatta gaagtgtccc tgtcatccat ggatgtggat
     1021 cacagtgata ttttatttct acagcaatgt ttggtggtgc ttctgtaaac aaaaattcgt
     1081 atatttctcc tgctttgact acaccagaag aaccaaagtc cccactgtat ccatctgtat
     1141 aacgatgatc aggtgtaact gtatgcggaa catcatctac attagtccat attacatgat
     1201 tatcgatagt caattgtacg ttaactaatt ttggtacata attatcctgc tgatcaggat
     1261 cagcagaacc aacaatcatt tctatatgtg tagatttatc aggatgcaaa acatgttcat
     1321 caacatcagg cgttgcaagt tgttcgggaa gataatacat ttgataataa ccaattccaa
     1381 ctgatgctgc aaccaacact gctagcaatc ctacaccata ttgatgacct gaagaaactg
     1441 tactcaactt gattcctccg actcgttagt gggtggtaat gacttttgcg tactttctac
     1501 agccttagta gaagaaattg aaggtggttt tggtatttgt tttgattgtg ccataccata
     1561 tctataaaga tggaaaaatc ctgcaaaagt gatgaggatt acccctgtca accacaaatt
     1621 aacattattc atgccagata gaaatgcgtt ataggcagct atattcagga aaacctgaaa
     1681 tgcaagcaac gctacaagta accaatttgt ccattttatg gatagattga ttggtgatac
     1741 tttgtgtggt gcactctctt tggcaagttt agcctttctt tctgcatctt ttgcaagatg
     1801 aatcatcatc aatgtaaagc caaagcttag cggtactaat aatatcataa ccagatagag
     1861 attgatcgga tcaattacca atctctctac aagtggaatt gtcgtatctg gtggaatgta
     1921 aaatccccag tatgtagtta ccaatatctg cgctattccg gtaattccca tagctgtaat
     1981 ccacgggcga tccttccatg agaatttttt atatctgtct atgaacgggg ttaccagtaa
     2041 agccagtatg aataaaccgg gccacaaaac tcctgttagg aatttatcat attgtgtcct
     2101 caaaaatgca tacaatcctg taagatacca ttcaggaact gttatccctg gaggtacagt
     2161 tggttcaaat ttgaaaccca agtcaacagg gaatacgcca ccagctaaac acaaagctcc
     2221 acatattgcc attaccattg gaacatcaaa caccaagaat cttgggaaat gtacagccat
     2281 taatccaagc ataacaagcg gtaggatgaa aacatgctgt gcataaaacc gaagcacaaa
     2341 atcatgaaag cccgcaccga acgcaagctc acgaatcatc ggaccggcaa ccggtataga
     2401 gtttgtaagt gatgcagcaa tactaattgc aagttctgct ctttcactga agattacatc
     2461 atatccagta aacgcttcaa gtattgttac agtgccaagt atcacacctg ttacccataa
     2521 aatttcattt cttattttgt atctgccact aaagtactga taatacatat gaagaatagc
     2581 caacataacc atggcatttg aaccatgata gtgaagattt ctgatatgaa atccaaatgg
     2641 caccgaatcg ttgatgaact gcacactatc ccatgaccta gtcatgattg gttcgtaata
     2701 gaacatcagt agagctccag tgagacctaa agtaacaaac atgatgaatg taagcatgcc
     2761 caaaaatcca aaggggctta cgaatcgtga cggaaatgaa aattttattg cagtaaaaat
     2821 tgttctgtct agctcattcc aaatccaata aaggaaattg gctgtactat tacgtctttc
     2881 aagcgaaacg gccataacct accactccat ttttattaac gccccatatt ggtggggaaa
     2941 taaacataaa accatctgaa tcagcctcga aatcaagttt tgcaagtgta ttagaaggag
     3001 gtgcttgtag tgatgcaggt cctaaatagg aagtaccagt tctaacatca tacatgcttc
     3061 catggcatgg gcattcccct ctcgtacgtc cttcctctgg ccaatacttc catagacacc
     3121 acagatgaag gcataccata ctgtatgctc taaatgcaga aacatttttt cttccaccac
     3181 caaatttttc tggaagtcta ataaactgcc attttctaaa agcttcctca ttcagaactt
     3241 catcagatgt ttctggataa gtaataacct cagcatggtt aattggataa gtgttaagat
     3301 ttgcttgtgt gccatctggt aatattacag gcactcttga aagggttgag gatgatggat
     3361 taggcagaaa tttgccccat ggaacaaaag gtgcaaacgc catggatgtt ccggcagcac
     3421 ccaaaagctt aagaaaatct cgcctggaca ttccaccagg tcctttttcc tgctcagaca
     3481 ttcaagctca aatgtcgaca aattggttat aaatcttgtt tcaaggtttt atcctgatca
     3541 tgtttacttc tggctttttt aatggcaaca aaagctacaa taattgtgat tatcacaatg
     3601 ctgatcacga ttatcaacat atttggttcc tgaatctctg gttttacatt ctccatagat
     3661 ttctggataa atgaatgatc gacgataaat ggaactgttt caactgcctc attgcctgca
     3721 agatctttag caactatctt gagatcatac gtaccagcaa aaacattcgt tgtattgtat
     3781 gaaagagatg ttatatcctc atacgaatca ccttgcggta gtaaaatttg gattgctcct
     3841 gtctcactca gatttttatc atcaatctta aaatcaattt ttaatgaatt tgatactgtt
     3901 gtattgtatt ttggcaagtt tagagtaatt ttgggtggag tattatcaac aatgaaagag
     3961 aacgtcttat cagcatcgtt ttcaagtatg tcgcttgcat gtatacgtaa ttcgtgtgga
     4021 ccatcattta atttccaatt catattacat tcattacata ccggtgtctc actttctact
     4081 tcatttccat caacgtaaaa ttttacaaaa ttaagatttt cttcaataat tactggtcta
     4141 attttgaaat tattattaat aaattcaggc atatcaaata ttatttcagg tggatcgtca
     4201 tcagcaagta tcgtatcaaa ttttactgcc aatgaaaaag gttcagtaat tgattcaccg
     4261 ttaaacagag ttgaatgaag taataatgtg tatgtacccg tttggtttat tggagcaaac
     4321 attactgttg atgtattatc tttatttttc acaggaaaga atccaccacc ttgactaaaa
     4381 ggtgtcactc caagccaatc aatagttggc caactccaaa aatgtccaaa aactcctgat
     4441 ggaacatttg tttgtatgat ttttccctgg ggatcaatca taaacgcaga aaaattagtg
     4501 tcatcgtttt cccatccaag atttattgac gcagaattta ttgtacggtc ttgtatatcg
     4561 aatgaatatt gcctccaatc acctgccata tacttacctg acatatcaaa agctcccttc
     4621 acgtaaccat ttccatacaa tgtgttaccc tctaacccat ctatcacaat ttgtttttct
     4681 ttttgatcaa ccacctttag tatattatat gaaacaggtg aacgaacatt atgatgctcg
     4741 ccttcaaaat ttaaaaatcc ttgatatata cctggatgtt tctctgcggg tggtgagatg
     4801 gttgcagata ttttagtgga attgtttggt ggtattgtaa tttcctgatg atcaacaact
     4861 acatcttccc acaaatcatt tttgtagtag cttgcagaga gtgtataatt cattgctgtt
     4921 gaattttgat ttgtatttcc tatccagtat gaaattctct caggtactgg atatacacca
     4981 atgacaggtt cgtcttcaaa tttttctact gggttagaca cacgaatttc ttgaactgtt
     5041 ccccaagatc cccctctatt tactaatgat aattcatcgc ttgatatttc attgtctttg
     5101 tttttatctc tccaatcata aacatataaa gaagaaattt ttaggtcgtc agcatagatg
     5161 gaatctgttt gattcatgaa tgtactaaat ggaaagttta ggttcaaaac cataagagat
     5221 gagtcaggat tgattatact atcttggtta ggttgtttgt atatactagt ttgatcagat
     5281 ggttcgtctt ttccaatgtc acttaatttt atgtaatttg gtctatatac tcccgtatcg
     5341 tttaagattg gatcctgaag gtgtactttc gtgacgccat ccatttgtaa tttctctatc
     5401 aattttagtg tgacagggtt aatcttgata tcaagtgatt tgtttgttgg attttctaat
     5461 gtgtaaatag tcgtggtttt ttctcctgga agcaatgttc caccatacca acttgtaatt
     5521 ggaaatgtat tatctgaaac tttagccttt tccagtccaa aaatttccaa atcaaataaa
     5581 gaaattggcc tatcaatcac ctttttgata tttgaaaatg attcgttatt gtggacaaaa
     5641 aatttcccac cttctccatt taccaccttg accgcactta acgcgtttac taatccggta
     5701 ccctgtgtaa atggatcatt ttgtagatca tctgtagtag acattagtat gttccttatt
     5761 gcaaatggat cataatctat ctgtctttca ttcaagcttt caataaccaa cgctgtagct
     5821 cctgcggtaa tcggtgcaga catactagta ccaccaaaaa gtgagaattg ttcttcatct
     5881 gtatctggtg ttattgttac agctgatggc acaaaactat aagcgccaat actcatcaaa
     5941 tcaggttttg gttcacccag aattcctgga cccctacttg agaaatctac aacgtgatca
     6001 gagtgagcag ttgtattgcc aaatcttggt tgctccttga atgaactata tccaacaaaa
     6061 tcattacttg tcacagcacc cacagatata ccatatgatg aaattcctgg actagctatt
     6121 gttccatatc catgccctga atttccagca ctggaaacta tagtcattcc gggataattt
     6181 tcgtgaagag attctggtgt agcaagtatg ttaacaatat gtgccaaaaa atcaaatcct
     6241 ggtccatatc ccaagtttgg aaatgtcgaa atcccccaac tattagaaat tatatttgcc
     6301 tttggctctc cagtgtaaac ccaactgtta tcttcatttt caaaccctgc aatccacatc
     6361 catgcataga caacatctcc agcccataat gccttgactg gaagaatttt aacgtctggt
     6421 gcaatcccct ttattgtata tttcttggtg tcattataga tatcatactc tgtaattccc
     6481 ttggaagcaa tagttgacga gcttgctgtc ccatgtccaa aaaaatcagt catcaaacca
     6541 aaaaacctac catccttatc cattggtgga agcaaagttc cattaactgc acctacatta
     6601 tcatcaattt cagccttttt tgaaaaaaga ccatacacat ccacaacgct tgctccaata
     6661 attcctgccg agtaatcatt tttaccgtca ccgtctgaat cataaatcaa taattcattt
     6721 ccatcaccaa ttgatattga agtttcatcg gtaaaatcaa aatcataatc tgccgatcgg
     6781 actccaggtt catctctgaa gaagtccatc caagaagttg acaagtctgg tgtgatggta
     6841 tcatagatgc cagcttcgtt tgggtcagtc accaaaacag gtacaacctg aactttgccc
     6901 atatgtgcat tcaggatata ccctagatga tatattccac tttgggattt gatgtacacg
     6961 cgttcagtat ttccaatttt gaaatcatgc tctactgtac cagctagaac ggttccaatt
     7021 ccagcatatg gatagaagga attgtaaaca gaaatttttg tattatttcc attctgtatc
     7081 atgtctaaaa acacgccctc atttgtcaaa tatacggtag aagtggcatc ctcggggatg
     7141 gtatgtgatt ttgtataatt ttttatcaag ccaaattcac ttttgtttgc ataaaatgtt
     7201 gaattggtca gtataatccc ctgaccatcc gcatcaagca tgacaggttc attcaaatga
     7261 tttcgtagca acgaatcagt gatatctgga tttgaaaaat caacaccagt atcaactact
     7321 gcaattgtca ctccgtctcc tgtgacatca taaagtgtgt gaactttttc tgattcagca
     7381 atgtttccaa tttgtgatat gtctgttgct gcatttgttg acaaatattt cgaatgaaaa
     7441 tccaacatat agtcctcgat aacataatat ccttttgatt ttagattaag tgcctcactt
     7501 tcatctaaaa taccaactga aaaaaatcca ctgttagaat taattccgta tacaatgttt
     7561 tttgtgtctt gatatgcatt attcagtgaa tcataaccga acacaagata tctttttggt
     7621 tgatcattta cattcgttat atccaacgaa attatgtctg atgttttatc aaaattaaaa
     7681 tttggagttt gtgatggttc aaaactttca ataaatccgt atgattcttc ttgcactagt
     7741 atgcatgtca aaaaaaataa tgtgaagaaa attatggttt tgaacatact tcaaacctcc
     7801 ttaaacgttt attttaatta tcttcttctt gctagtattg ttatctgtag agctagtatc
     7861 acagctatag atgcaactat aggaaatagt aatgaattta cctgtaatga tgcttcctca
     7921 atcgtagtta ccgcctttga cattgatgat acactattat caatttcagt tagacttcca
     7981 cgaattttgt cgctagcacc ttcaaatgta tcaccaaatc catgtatctc agtttgtaat
     8041 gttccaactt cgtcagatgc agtagttatt acttcattta gagttactac ctgacgagac
     8101 agtccagcta tgtcctgtcc taaaacaaga gttgctgcag aaccatgcga ttgtgttcct
     8161 tggttaaatg ctaccatatg gaagatgtac gtaccctcaa gtgttggagt atgttctaca
     8221 aagaacaatc cttcatgtaa catgcttaat gatttgatca tagattgcac ttttccgtca
     8281 ggagaatgca agtgtgatga tgatagtaaa cttgcagcct catcatttac cataagacca
     8341 tcacttgtga cttgaacata aatacgaaaa gatttgtgtg tagctgcaat ttctggtgcg
     8401 aagacctgta cgttcaggtc acgttctatt ggtatttttg taacctctgt ggttgatgca
     8461 aatttcacat caatttttac tgatgttcca tctagtgtga tggattcaac agtataagtt
     8521 ccaaaaggaa atgttgttga agaagatggc cattccagta attttacacc gtatgtacca
     8581 tcattatttg tggacacttg ctgaaattcc gtgatggaac cgtcgggtga aaagaggcgt
     8641 acaactaaag gctgaccggc tggtgcatca ccatatacat aaagtgggtg ccgttcggtg
     8701 tatatttgct gattagtaaa caaggtgagt tcatctgcaa atgcctgtgg taaagtaaac
     8761 acaattaata caaatacagt tgctaatcct aatagtttca attgtatttg atgatttttg
     8821 atcataaata gggtttatga ataaattact ttagttatcg aagttaaaaa aaagaaaagg
     8881 ggggggttag atagtttcta gtcttatacg acgtctatgt ttacagatac ggttggagac
     8941 aacgctgttg gattatcaac actctgccat acaaacactg ttgcagtgta agagccagaa
     9001 gctgatggtg tccatgacaa tgctggactc attgattgtc cagaagtcaa agcaccggta
     9061 atccaagcga gtgagactgt aacaccgtta gcatcttgta cttgtaccaa gtaagcgaat
     9121 gactgatctt tgcccgttcc gttagaaacg tctgcagcaa tctgtacttg ttgatccgct
     9181 gatacttcag agacctggct tccgaatgca tcgactacgc gtgcattaga tgctggggct
     9241 ctctcaagtg gtggataaac tgtaccgact gttgtagttg cggcaaaggt caatgtgtct
     9301 gcgctttcat atggttcagg taatgtttgg tcagtatatt ctacagtgac ggtatcacct
     9361 tcagaaactc gtagcaatga accagttgat ttgtcatctg cagtaaagaa tactgtagtt
     9421 tcaaatacac cagttgcttc gccggtttca tgtaaggtta ttgatatacc accagagtca
     9481 gaatctgaga atacatttgc tgttttagtg tcaattacgt caggattttt gtctaagtca
     9541 ccatcaacaa gagtaatagt agaagagcca cctgggctta cactactatc tccgaagctg
     9601 atttcaccaa tattccatgc gattagcgcg gatgctagtg aaacactacc atctgtgtat
     9661 tcgtatgata ctgtgatacc gtcagtttga cctgcggtat tgatcacacc atcggtgtta
     9721 ccagttgcgg aatctgctgt taatgctgtt tcaaagacgt ttccgtctgc aaagcctttg
     9781 agtacatatt caccgtaata aactcctgtg tcttcaccag tttctctgag agtgtaagaa
     9841 gttgatccag ttgggaatgt ttcctgtctg gttgatactt taatcggtag agcggttgta
     9901 ccaatatttt ctattttctc tgagtcttgg ttatgatcag gtgctgttac tgtaattaat
     9961 actctgtctg tccaatcgta aattgctcga tctaattcga cgatagcgcc gaagttagat
    10021 gcatagatag tggcttcaat atcgttgtaa tcatcgtgta cctcatcctt gcctgaaagg
    10081 ccttgatccc tgtatgtgat cgtcacttct tctccttgtt caatagatgt tgaacttatt
    10141 gaggatggga aggtgaacac tgtctggaag acaccagtat tgctaccggt ctctataaac
    10201 ttggatggca ttgctgcgaa ttcagaattg ctcatcaatc ttgatgaact tgcagaagaa
    10261 tcccattcaa ccagttctgt agagtaagat tcggagctat cactgtcaag gttcaagtct
    10321 gggtcagtta tcgttacgat aatgtctgat ccaatgacat aaacttcttt gtcagttgat
    10381 atagaacctg ttctcaaatc tacagttgcg gaatcgtaga atacggtatt aacacctgcg
    10441 tcgtcagttt gatcgtcata tgctgcttgt aagaccatac cagaagttac gttgaatgct
    10501 gcacatgtgc ttgttgcaca tgttccagtt actccatcgt aaattgtttc ctggtgatct
    10561 agtgagaagc ttccttcata tacgctagtt cctcgttcga cttcggtcaa aggaccggtt
    10621 tcaccacttg tgaggtcagc tgctgctgct ccacctgtgg ctattacatt accattgagt
    10681 ttgatgtaaa ctctgtcagc agcagatgtg attgtttgac ccacttcatc gccgtcgctt
    10741 actgtgaagt aaactgtgac gttaccgtga gttgcacccg ctgttggatc tggtgatgtg
    10801 tcgaatggat cgtctagtgt gtcgccagat ccatcagtta gccatccgtt aaagaatggt
    10861 actggataga cctgtctatc aaaggaaatt gagccagact cggttgcaat tttgactgaa
    10921 gtgtatacgt caacagtggt gccggaggca tctttattgt cgtagtatgt aaacttcaaa
    10981 tcatttccaa tgttaccaga gctggtcaaa aattgaccaa caaatacacc tgtatcatct
    11041 cctgtttcca caagcttgag gttagcgata ctagtgatag tatcgttgta tgatacagcg
    11101 aatgtatcac ctgcgttagc tgttgttcct acttcacgtt cttgtgagta
//
LOCUS       DASCPJ010000033        10387 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000622255, whole genome shotgun sequence.
ACCESSION   DASCPJ010000033 DASCPJ010000000
VERSION     DASCPJ010000033.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000622255"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(121..1218)
                     /gene="aroC"
                     /locus_tag="QGF01_01685"
     CDS             complement(121..1218)
                     /gene="aroC"
                     /locus_tag="QGF01_01685"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HJN19643.1"
                     /translation="MVVGSSIGHNLVLTSFGESHGKCVGAVLDGCPAGLELQEKDIQK
                     MLDLRRPGQSLVSTQRKEGDVVEILSGVFRGYTTGAPITTIIWNKDHDSKSYDKLRTA
                     MRPGHSDYPAFVKYKKFNDHRGGGRFSGRLTATHVMGGAIARKLLKVTLGIETNAYTC
                     KIGKIGMKNQATKQSLKSIYTNDVRCPEKTIAGKMKKSILDARKKGDSLGGIVESVTN
                     NIPIGLGEPIFNSLESELSKAIYAIPAVKGVEFGSGFSGSEKHGSQNNDSYTFRNGKV
                     VTKTNNAGGILGGISNGMPIIMRVAFKPASSIGKIQETVDIKTKKNTKLRVEGRHDPC
                     VVPRAPPVVDSIVSLVIADQALQGGFIKPVI"
     gene            complement(1270..2541)
                     /gene="aroA"
                     /locus_tag="QGF01_01690"
     CDS             complement(1270..2541)
                     /gene="aroA"
                     /locus_tag="QGF01_01690"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HJN19644.1"
                     /translation="MKCKVEKSKISGHIICPSNKSYTHRAVFIASLSDGKSIIKNILK
                     SSDTIATINACKNFGVKLTESENKIIIKNSIDSKVEGCIIDACNSGTTIRIAAAIAVL
                     SHGKTVLSGDDSLKKRPMKPILDALESIGAKCSSNEGKPPISVEGEVKGGEVQISGSI
                     SSQFISALMIIAPRLDNGLVLNIQNELVSKPYLDLTIATMDKFGASVKTEIPYQKYII
                     KHQNYKATNFTIPSDFSSLALLLSAAVLLGNELEIEISMGDMPQADEAIIDILEILGV
                     IITLEKNTIKIKSSEKLAGGKFDLSDSPDLLPAIAILSLKSAKPIEIFNVKHARYKET
                     DRIAILARELAKLGINVTENDDGLILKPAKKLVSATLCSENDHRLFMAFCIAGMYVGD
                     CTITDPESVEISYPNFVSEMKKIGGKIFPDE"
     gene            complement(2531..3385)
                     /locus_tag="QGF01_01695"
     CDS             complement(2531..3385)
                     /locus_tag="QGF01_01695"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HJN19645.1"
                     /translation="MVSVQSSIHSAISIVNAIATGKGATLGISKKIDIIIKEMPGKGI
                     VIESDGETISSRLINEVIKQIVPVNELAKTKLKVSLNSEIPTGYGLKSSSAISSAVSL
                     GCAKLFRPDMNETEILRIGVDASIKTKVSLTGAYDDACACYYGGFNVTDNYKKKIIHS
                     EKCLDDISAIIFLPKSRKRGKIKKLVTLSSVFERAWNFAKNSDYWNAMIINGLATSAI
                     LDSDPKIISKLIENGALGASISGNGPSIAAIAKNDVLSDVKKVFAPLDGNVTITKINN
                     KKAEVYEV"
     gene            complement(3385..4206)
                     /gene="aroE"
                     /locus_tag="QGF01_01700"
     CDS             complement(3385..4206)
                     /gene="aroE"
                     /locus_tag="QGF01_01700"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HJN19646.1"
                     /translation="MTKTYAIIGDPIDHSLSPLIHNAAFRSLNLDSTYIAYRIQKNEL
                     EVGINALKKIKIAGFNVTIPHKTEMMKFLDETDDICNMCGAVNTVVNNDGVLKGYNTD
                     VDGFLDPIKKRKITIKDSKVLILGAGGAARAIIAGMVKEKADKITISNRTLKNATELV
                     QFTKNLGGNATAIPLEEIDQFISEHKFIINSTSVGLRNESSLIPTDQIDKNSIVYDIV
                     YTPMNTNLIVNAKKNGATIIYGYEMLLSQASHAFEIWHDVKAPYEDMKKAIFGGM"
     gene            complement(4207..4875)
                     /locus_tag="QGF01_01705"
     CDS             complement(4207..4875)
                     /locus_tag="QGF01_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HJN19647.1"
                     /translation="MIYKTCVSIGEKTPKKISKSLSKSLKKSDHAEIRFDFLNPELVP
                     ETLDMIKKDLKKCVCTLRPSSEGGRFSGTEKNRISIIKLIAEYNPFLLDIEFNTIKKN
                     KNLLRYLKSTNTDLLVSWHNFKQTPNASMLKKQYFKMKKFSNNIKIVTTAKTVNDIPK
                     ILSLYDNKNTRLIAFCMGDIGKVSRLLSLFFGSPFTYVSLGKPIAAGQLKLDELKSML
                     MRSG"
     gene            complement(4877..5932)
                     /locus_tag="QGF01_01710"
     CDS             complement(4877..5932)
                     /locus_tag="QGF01_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HJN19648.1"
                     /translation="MINNKQLIIQPKVGKNQLVKFVNNLEAEGIKTIFADPKILKKTK
                     IQTIFPSQDANYVILDKDVIKKSKGKKVGRRFKVSSNKDIEKILDSAKKGLDFVIIEV
                     KDWKIIPLENIIAKLHKLHTQIFAIANNPKEARKMFSILDVGVDAVIFNTGSMNEIRE
                     ALVYLGSKSFELSVAKIIDIQEVGDGERVCIDTASMLNKGEGMLIGNRANFLFLIHNE
                     SVGSSFTSPRPFRVNAGAVHCYTLSPDGTTKYLSELETGVEVLVLNTKGKARRATIGR
                     CKIEKRPMLMIKAKVGEEIGGIIAQDAETIRLVKSNGHLISVTHLKKGDSVLVHSKTA
                     TGRHFGMEVSDEYILEK"
     gene            complement(5919..6707)
                     /locus_tag="QGF01_01715"
     CDS             complement(5919..6707)
                     /locus_tag="QGF01_01715"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HJN19649.1"
                     /translation="MTTGTEIRLNRILRKGRMLCIPMDHGISNGPIIGLEKPHSMIYK
                     CESHGISCVIINKGIIKTLPRPPKVGILVHFSASTSLSTAPNRKMLTGSVDEALRLGA
                     DGVSLHINIGGKEEPEMLADLGNISEQCHKWNVPLLAMMYPRGENIKNPHDPEIIAHA
                     ARIGAECGADIVKTLYTGDIDSFSKVVESIPVPVVIAGGAKMETDMDVLQMTEDAMKA
                     GAKGVTYGRNIFAHRSPEKIVEALSGIIFKNQTAKEVIDTIDKQ"
     gene            6809..7936
                     /gene="mqnC"
                     /locus_tag="QGF01_01720"
     CDS             6809..7936
                     /gene="mqnC"
                     /locus_tag="QGF01_01720"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HJN19650.1"
                     /translation="MSQILNQIHSSDIKDILENSLNGIRPGPDDCIRLLESDDVHLMG
                     LTAGTLTRKKFGQKASFVNNIILNYTNVCITDCKFCAFYRPPNHEESYTLGLDEIELR
                     IKTAWDLFKIRQVLIQGGHNPDLGIEYYENSFKMIRKKFPQVGVHGLSVSEIDTIAKV
                     EKSSTKEVLSRLKDAGLQSLPGAGAEILNDQVKEIISPKKIMSKEWLDIMEEAHTLQI
                     PSSATMMYGHVESNKQIAEHFEKIVHLQEKTNGFMAFIPWSFEPNNTVMQKDGTITSG
                     AGGYQLLKMIAISRLVFDGLISHIQSSWLTNGVGMAQLALQYGADDFGGTLIGEEVVS
                     CTGSRSTELTSRQIIDSIHQIGFEVEERDNFYQTVCNYQIP"
     gene            complement(7924..9366)
                     /locus_tag="QGF01_01725"
     CDS             complement(7924..9366)
                     /locus_tag="QGF01_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HJN19651.1"
                     /translation="MTIESIQHFIDELEKAGELKRVKTEVDTNLEIAEILRRVSYTDG
                     PAVLFENVRNYEIPVLGNAFGSIKRLEIGLETKDFSEIGQRIVDMTKMEIPSGVFNKI
                     RKLPELSKMSESFPKLEKSGPVTDIINNSPTFDKIPILKSWPKDAGKFITFGLVATKH
                     PETGVRNLGVYRIQIIDDTHALMHWQKHKRGAAHHDLLKEKNNKIEAAIIIGGEPATV
                     FSAVAPVPEGLDKYLFAGITRKKGIKTVKCKTIDLEVPANAEIVFEGYVDAMDVRGEG
                     PFGDHTGYYTPKEPFPTFTLTGIMQRKNPVYLTTVVGKPILEDAYIGKVIERSFLPLI
                     KMLHPEVVDFSMPPAGWFQGLAIVSIKKRYPGQAKKVMMGLWGMGQLALTKMIVVVDS
                     DVNVHNVNEVIWAITTRSDAARDTTIINNTPTDTLDPASPKVNLGSKLGIDATQKTLE
                     EGFEREIQEEVKVDESTKEMVDSKWSNYGI"
     gene            9476..9709
                     /locus_tag="QGF01_01730"
     CDS             9476..9709
                     /locus_tag="QGF01_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19652.1"
                     /translation="MAEKQKCVVCSGKGKIELLDTQCPFCNGTGEYTKISEKYFESHI
                     CQCIFLDRKICPLCEKKCHHDTPNKPKLLIGPM"
     gene            complement(9719..10093)
                     /locus_tag="QGF01_01735"
     CDS             complement(9719..10093)
                     /locus_tag="QGF01_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19653.1"
                     /translation="MDDFEEDFPDFDWKNTPAYKHPGGKNCPCPKHEYIREQVNFTKQ
                     VNDSGKDARVTLKFCPEHYSVYINEVIPAMPMKYKILTKIALKIGAIKIVILKHMQSD
                     LCFYCKFGSGGHDKKNELPPMP"
     gene            10182..>10387
                     /locus_tag="QGF01_01740"
     CDS             10182..>10387
                     /locus_tag="QGF01_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HJN19654.1"
                     /translation="MAKAKKKSRKVSRKTARKTARKPARKPARKPARKTARKAAPSKK
                     ALESKIAALERKLSNLSKPKPQTQA"
BASE COUNT         3171 a         1898 c         1571 g         3747 t
ORIGIN      
        1 ccaagacttg ctttggcatc gcctatctgc ttggcaatgt ctgttcttgt cttcaacaac
       61 ttgatcatct caagcgttat ctcttcaatt ttcttacgat gttcctctat atctgacatt
      121 ttagataact ggttttatga atccgccttg taatgcttga tccgcaatta ctagcgatac
      181 tatagaatca acaacaggag gtgctcgtgg cactacgcat gggtcatgcc ttccctctac
      241 tcttaatttt gtatttttct tagttttgat atcaacggtt tcctgaatct tgccaataga
      301 tgaagctggc ttgaaggcaa ctcgcattat tattggcatt ccattcgata ttcctcctaa
      361 aattccacca gcattgtttg tcttggtaac caccttgcca ttacgaaatg tgtatgaatc
      421 attattctgt gagccgtgtt tctcagatcc tgaaaatcca gaaccaaatt caacaccttt
      481 cacagctggt attgcataaa tggctttact gagttctgat tcaagtgagt taaaaattgg
      541 ttcacctaaa ccaataggaa tattatttgt tacagattca acaatacctc ctaatgaatc
      601 accctttttc ctggcatcga gtattgattt tttcattttt cctgcgatag ttttttcagg
      661 acatctaaca tcgttggtgt atattgattt gagtgattgt ttagtagctt gatttttcat
      721 tcctatcttt ccaattttgc atgtgtacgc atttgtttca ataccaagag taactttgag
      781 taatttcctc gcaattgctc cacccattac gtgtgtagct gtcaatctac cagaaaatct
      841 tccaccgcca cgatgatcat tgaatttttt atattttaca aaagcagggt aatcagagtg
      901 accaggtcgc attgcagttc gtaatttatc ataacttttt gaatcatgat ccttattcca
      961 gatgattgtt gtgatgggtg caccagttgt atatcctcta aacacacctg aaagaatctc
     1021 tacaacgtca ccctcttttc tctgtgttga aactaatgat tgaccaggtc ttcttagatc
     1081 aagcattttt tgtatgtctt tttcttgtaa ttctagacca gctggacagc catcaagtac
     1141 tgctccaaca catttaccat ggctttcgcc aaaacttgtt aagactaaat tatgtccgat
     1201 tgaacttcct accacaatat aatattattt aagacaaata taactttaca atctataatt
     1261 aatcagcatt cattcatctg gaaaaatttt accaccaatt tttttcattt cagatacaaa
     1321 attaggatat gaaatctcta ctgattctgg atcagttata gtacaatcac caacatacat
     1381 accagcgata caaaaagcca tgaataatct atgatcattt tcagagcaaa gtgttgcgct
     1441 tactaatttt ttcgctggtt ttaggattaa accatcgtca ttttcagtaa cattgattcc
     1501 taatttggct agttctctag caagtattgc aatcctatca gtttctttat acctagcatg
     1561 tttaacatta aaaatttcaa ttggttttgc cgattttaat gaaagtatgg caatcgcagg
     1621 gagaagatct ggtgaatcgc ttagatcgaa ttttccaccc gctaattttt cagacgactt
     1681 gatttttatt gtattttttt ctaatgtaat aataacacct aaaatttcta atatgtcaat
     1741 tattgcttca tccgcctgag gcatatcacc catactaatt tcaatctcaa gttcatttcc
     1801 caataatact gcagctgaaa gcaataatgc taaactagaa aaatcagatg gaattgtgaa
     1861 atttgtagcc ttgtagtttt gatgttttat gatgtatttt tgataaggaa tttcagtttt
     1921 tacactggca ccgaatttat ccatcgttgc aattgtcaga tcaagataag gttttgacac
     1981 caattcattt tgaatgttta aaacaagtcc attatctaat cttggtgcaa taatcatcaa
     2041 tgctgaaata aattggcttg agatggaacc agatatctgt acttcaccac cttttacttc
     2101 tccttcaact gaaatagggg gttttccctc gtttgaacta catttagcac caattgattc
     2161 cagtgcatca agtattggtt tcattggtct ctttttcaga ctatcatcac ctgataatac
     2221 agttttacca tgagatagaa ctgcaattgc cgctgcaatt ctaatggttg taccagaatt
     2281 acatgcatca attatacatc cttcgacctt cgaatcaatt gaatttttta taattatttt
     2341 attctctgat tcagtaagtt ttacaccaaa gtttttacat gcatttatcg ttgcaatggt
     2401 atcacttgat tttaaaatat ttttgataat acttttacca tctgatagtg atgctatgaa
     2461 tacagctcta tgtgtgtaac ttttgtttga tggacatatg atatgtccag aaatttttga
     2521 tttttctact ttacacttca tatacttcag cctttttgtt atttatttta gtaatagtta
     2581 cattaccatc aagtggagca aaaacttttt tcacatctga caaaacatca tttttagcaa
     2641 ttgcagcaat tgatggacca ttaccagaga ttgatgcacc taatgcacca ttttcaatca
     2701 atttggagat aattttagga tccgaatcta ggattgcaga cgtggcaagt ccattaatga
     2761 tcattgcatt ccagtaatca gaatttttcg caaaattcca ggcacgctcg aaaacactac
     2821 tcagagtaac cagttttttg atttttcctc tttttcttga ttttggcaag aaaattatag
     2881 cagaaatatc atctaagcat ttttcagagt gtatgatttt cttcttataa ttatcagtga
     2941 cattaaatcc accgtaataa catgcgcatg catcatcata ggccccagtt aaactaactt
     3001 tagttttaat tgatgcatcc acaccaatcc gtaatatctc agtctcattc atatcaggtc
     3061 taaataattt tgcacatcca agagatacag cagaagaaat tgcgctggaa ctttttaacc
     3121 cataaccagt tggtatttct gaatttaatg aaacttttag ttttgtttta gcaagttcat
     3181 taacggggac tatttgtttt attacttcat tgatcaaacg tgagctaata gtctcaccat
     3241 cagactcaat tactattcct tttccaggca tttcttttat gataatatct attttttttg
     3301 aaataccaag cgtggcaccc ttacctgtag cgatagcgtt tacaatagag atcgcactat
     3361 ggatagatga ttgtacagat accattacat tcctccaaat atcgcctttt tcatatcttc
     3421 ataaggagct tttacatcat gccaaatctc aaatgcatga gacgcctgtg ataaaagcat
     3481 ctcataacca taaattatag tagcaccatt cttttttgca ttaacaataa gatttgtgtt
     3541 cattggagta taaactatgt cgtatacaat cgagttttta tcaatttgat cagtaggaat
     3601 taagcttgat tcatttctta atcctacaga tgttgaattt ataatgaatt tatgttcaga
     3661 aataaattga tcaatttctt ctaatggaat cgcagttgca ttaccaccaa ggttttttgt
     3721 gaattgtact aattcagttg cattttttaa ggttctattt gagattgtta ttttatccgc
     3781 tttttctttt accattccag ctataattgc tcttgcagca ccacctgcac ctaaaattaa
     3841 cactttggaa tccttaatcg tgatttttct tttttttata ggatctaaga aaccatccac
     3901 atcagtattg tatcctttga gcactccatc attatttact actgtattta ctgctccgca
     3961 catattgcat atgtcatcag tttcatctaa gaatttcatc atttcagttt tatgaggaat
     4021 tgttacatta aatccagcga ttttaatttt ttttagagca ttgattccta cttctaattc
     4081 atttttttgt attctatatg caatataggt agagtctaag ttcaaagaac gaaatgcggc
     4141 attatgtata agcggtgaca aggaatgatc aataggatca ccaatgattg catatgtttt
     4201 agtcatttat ccacttctca tcaacattga tttcagttca tccagtttaa gctgtcctgc
     4261 tgcaattggt tttcctaatg atacataggt gaatggacta ccaaaaaata aactgagaag
     4321 acgtgatact tttccaatat cacccatgca aaatgcaatc agtcttgtat ttttgttatc
     4381 atataacgat agtatttttg gtatgtcatt aactgttttt gctgtcgtaa caattttaat
     4441 attgtttgaa aattttttca ttttaaaata ttgttttttc agcatggatg catttggagt
     4501 ttgtttaaaa ttatgccatg atacaagtaa atccgtattt gtacttttga gataacgtaa
     4561 tagattttta ttttttttaa ttgtattaaa ttctatatct aacaaaaatg gattatattc
     4621 tgcaattaat tttataattg atattctatt tttttcagtg cctgaaaacc ttcctccttc
     4681 tgaacttggt cttaatgtac agacacattt tttcaaatct tttttgatca tatctaatgt
     4741 ttcaggcact aattcaggat taagaaaatc aaatctaatc tcagcatgat cagatttttt
     4801 tagtgatttg gaaagactct tactaatttt ttttggtgtt ttttcaccaa tactaacaca
     4861 tgttttgtat atcatatcac ttctctaata tgtactcgtc agatacctcc attccaaaat
     4921 gtctaccagt agcagttttt gagtgaacta gcactgaatc cccttttttt agatgtgtta
     4981 cagatatcaa atgaccatta gattttacta aacgaattgt ttctgcgtct tgcgctataa
     5041 taccaccaat ttcttcacca acttttgcct taatcataag cattggtctt ttttctattt
     5101 tacatctgcc tattgtagca cgtcttgctt tccctttcgt attaagtaca agtacttcta
     5161 caccagtttc taattcagat aggtacttgg tcgtcccatc aggtgataat gtatagcagt
     5221 gaactgcacc tgcgttaacc ctaaatggtc taggcgaagt aaatgatgaa ccaactgatt
     5281 cattatgaat tagaaacaaa aaatttgctc tgttaccaat tagcattcct tcacctttat
     5341 tgagcataga tgcagtatca atacataccc tctcaccatc tccaacttct tgaatatcta
     5401 tgatttttgc aacactaagt tcaaaacttt ttgaacctaa atacaccaat gcctctctaa
     5461 tctcattcat tgaacctgta ttgaaaatca cagcatcaac tccaacatcc aaaatagaaa
     5521 acatttttct tgcctctttt ggattattcg caatagcaaa aatctgtgtg tgaagtttgt
     5581 gtaattttgc aataatattt tccaatggaa ttattttcca atcctttact tcaattatca
     5641 caaaatccaa tccttttttt gccgagtcaa gaattttttc tatatccttg tttgaagata
     5701 ctttgaaccg tctcccaact ttttttcctt ttgatttttt gattacatct ttatctaaaa
     5761 taacataatt agcatcttgg gaaggaaaaa tcgtttgaat ttttgttttt ttcaggattt
     5821 taggatcagc aaatatagtt ttaattccct cggcttctag attgttcaca aacttgacta
     5881 gttggttttt accaactttg ggctgaatga ttaattgttt attgtttatc aattgtgtcg
     5941 atcacttctt ttgctgtttg atttttgaat attattcctg aaagagcttc aacaatcttt
     6001 tctggagatc tatgtgcaaa tatatttcta ccatatgtca cacccttagc tcctgccttc
     6061 atggcatctt ctgtcatttg taaaacatcc atatcagttt ccatttttgc tccaccagca
     6121 attactaccg gcacaggtat gctttccaca acttttgaaa aagaatctat atcaccagta
     6181 tacaatgttt taacaatatc agcaccgcat tcagcaccaa ttctagctgc atgagcaatt
     6241 atttcaggat catgtggatt ttttatattt tcacccctag gatacatcat tgccaataaa
     6301 ggaacattcc atttgtgaca ttgctctgaa atatttccca agtcagcaag catctcaggt
     6361 tcttcttttc caccaatatt gatatgtaat gaaacaccgt ctgcacctaa tcttaatgct
     6421 tcatcaacag aaccagtaag catctttctg tttggtgcag ttgaaagtga tgtacttgca
     6481 gaaaaatgca ctaatattcc aactttaggt ggtcttggta gagttttgat aatacctttg
     6541 ttaattataa cacatgagat tccatgactt tcacatttat agatcataga gtgtggtttt
     6601 tctaaaccaa taataggacc atttgagatt ccatggtcca taggtataca aagcattcta
     6661 ccttttctta gaattctgtt tagtctaatc tcagttccgg ttgtcataga tggtataatt
     6721 tttgcatctt acttaaaaat tatagcgaat tgatttcttc gctttctaac gattaaatag
     6781 tcataatcac taccaaaatt ggggttaatt gagccaaatt ctgaaccaaa tccatagttc
     6841 tgatatcaaa gatatacttg aaaattctct aaatggaatt agacctggac ctgatgattg
     6901 tattagacta cttgagtcag atgatgtgca tttaatgggg ctaacagctg gaactttgac
     6961 acgtaagaaa ttcggtcaaa aggcatcatt tgtgaataat ataattctaa attacacaaa
     7021 tgtgtgtatt actgattgta agttttgtgc attttataga ccacctaacc atgaagaatc
     7081 ttacacattg ggtttggatg aaattgaatt acggattaaa accgcatggg atttatttaa
     7141 aattagacaa gtcctaatcc aaggagggca taatccagat cttggaatag aatattatga
     7201 aaattcattc aaaatgatta ggaagaaatt tccacaagtc ggtgttcatg gtttatctgt
     7261 atcagaaatt gatacaattg ctaaagttga aaaatcatca accaaagaag tattatcaag
     7321 attaaaggat gcgggtttac agtcattacc tggtgctggc gctgaaattc taaatgatca
     7381 agtaaaagaa atcattagtc ctaaaaaaat tatgagtaaa gaatggttag atattatgga
     7441 agaggcacac actcttcaaa ttccatcatc tgctacaatg atgtatggtc atgtagaatc
     7501 aaataaacag attgctgaac attttgaaaa aattgttcat cttcaggaaa aaactaatgg
     7561 gtttatggcg tttattcctt ggagttttga acctaataat acagtaatgc aaaaggatgg
     7621 cacaatcacg tctggcgctg gaggatatca actcctaaaa atgattgcaa tatctagact
     7681 tgtatttgat ggacttattt cacatattca gtcatcatgg ttaacaaatg gagttggaat
     7741 ggcacaatta gcattacaat atggtgctga tgattttggt gggacattaa ttggagaaga
     7801 agttgtatca tgtactggtt ctcgttctac agaacttaca agcaggcaga ttattgattc
     7861 cattcatcaa attgggtttg aagtagaaga aagagataat ttctatcaaa ctgtttgtaa
     7921 ttatcaaata ccgtaatttg accactttga atcaaccatt tcttttgttg attcatccac
     7981 tttgacttct tcttggattt ctcgctcaaa cccttcttcc aaagtttttt gcgtcgcatc
     8041 aattcctaat tttgagccca aattaacttt cggtgaagca ggatctaagg tgtcagtagg
     8101 tgtattattt atgatggtag tgtctcgtgc agcatcagat cttgtagtaa ttgcccaaat
     8161 cacttcattg acattatgta catttacatc actatcaact actacaatca tcttagtcag
     8221 tgctaattgt cccatacccc ataaccccat catgactttt tttgcttgac caggatatcg
     8281 ttttttgatt gacacaatag ctaatccttg aaaccatcct gcaggaggca tactgaaatc
     8341 tacaacctcc ggatggagca ttttaattaa tggtaagaaa gagcgttcta ttacttttcc
     8401 aatatatgca tcttccaaga ttggtttacc aacaacagtt gttagataaa ctggattctt
     8461 tcgctgcata attcctgtga gtgtaaatgt tggaaatggt tcttttggtg tatagtaacc
     8521 agtatgatct ccaaacggtc cttcacctct cacatccatt gcatcaacat atccttcaaa
     8581 cacaatttca gcgttagcgg gtacttcaag atctatagtt ttgcatttga cagttttgat
     8641 tccttttttt ctagtaatgc cagcaaaaag atacttatca agaccttctg gtacaggtgc
     8701 tacagcagaa aatactgttg caggttctcc accaattatt atagcagctt cgattttgtt
     8761 atttttttct tttagtaaat catgatgtgc agcacctctc ttgtgttttt gccaatgcat
     8821 taatgcatgt gtatcatcaa taatttgtat tctatataca cccaaatttc gtacaccggt
     8881 ttctggatgt tttgttgcaa ctagaccaaa cgtaatgaat tttcctgcat cttttggcca
     8941 tgattttaaa attgggattt tatcaaaagt cggtgaatta tttatgatgt cagtaactgg
     9001 accacttttt tccaattttg gaaatgattc actcattttt gacagttcag gtaattttct
     9061 aattttatta aaaacaccag atggaatttc catctttgtc atatctacaa ttctttgacc
     9121 aatctcagaa aaatctttag tttctaatcc aatttcaagc cgtttaattg aaccaaatgc
     9181 gttgcctaac actggaattt catagttacg tacattttca aaaagaacag ctggaccatc
     9241 agtgtaagat acccttctta aaatttcagc aatttctaaa ttagtatcaa cttcagtttt
     9301 tacccttttt aattcaccag ctttttctaa ttcatctatg aaatgctgaa tactttcgat
     9361 agtcacatta ctcatttatg gtatgatagt ataagcttaa ctcgatttac atacctcatt
     9421 tcctaaattt tttttacata aatattttaa ttcaataaaa cttgaattct aattaatggc
     9481 agaaaaacaa aaatgtgttg tatgtagcgg taagggtaaa attgagttgt tagatacaca
     9541 atgtcctttt tgtaatggca ctggtgaata tacaaaaatt tcagaaaaat attttgaatc
     9601 tcatatctgt caatgcattt ttttggatag aaagatatgc cctttatgtg aaaaaaaatg
     9661 tcatcatgat acaccaaaca aaccaaaatt attgataggt cctatgtagt tttttatgtc
     9721 agggcatagg tgggagttca tttttcttat catgtccacc cgaaccaaat ttacagtaaa
     9781 aacacaaatc agattgcatg tgttttaaaa ttacaatttt tatcgcccct attttcagtg
     9841 caatctttgt caagatttta tatttcatgg gcatggcagg tatgacttca tttatgtata
     9901 cagaataatg ttctggacaa aatttcaaag tgaccctagc atcttttcca gagtcattaa
     9961 cttgttttgt aaaattaact tgttctctta tgtattcatg ttttgggcaa ggacaatttt
    10021 ttcccccagg gtgtttatat gcaggagtat ttttccagtc aaaatctggg aaatcttctt
    10081 caaagtcatc cataatatca atattcgttg tgattataaa aattcgatct agactgatta
    10141 taagcacaat ctaaataaag tagatatcaa gaaggtagca tatggctaag gctaaaaaga
    10201 aatctagaaa ggtatctaga aaaacagcta gaaaaacagc tagaaaacca gctagaaaac
    10261 cagctagaaa accagctaga aaaacagcta gaaaagcagc accttcaaag aaggctcttg
    10321 aatcaaagat cgctgctctt gagagaaaac taagtaatct ttctaaacca aaaccacaaa
    10381 cgcaagc
//
LOCUS       DASCPJ010000034        11178 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000626571, whole genome shotgun sequence.
ACCESSION   DASCPJ010000034 DASCPJ010000000
VERSION     DASCPJ010000034.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000626571"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="QGF01_01745"
     CDS             <1..194
                     /locus_tag="QGF01_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19655.1"
                     /translation="PKKEAPKEEPKKEAPKEEPKKEAPKEEPKKEAPKEAPKEEPKKE
                     APKEAPKEAPKKEPKEKKA"
     gene            226..1488
                     /locus_tag="QGF01_01750"
     CDS             226..1488
                     /locus_tag="QGF01_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HJN19656.1"
                     /translation="MHWKDTLPDWYIKKYGKQPCVNIGTAGHVDHGKTTLIQALTGSW
                     TSVHSQELKRGITIRVGYSDAAFYKCKKCESPLGYSTEPKCPNCGKESELSRVVSFVD
                     SPGHESLMANMLSGSALIDGAMLLVAANSKVPQPQTKEHLLALQTLGIKQIVIVQNKV
                     DLISYQDALSNFSEITKFVKGTKHADSPIIPVSAQSSLNVDALIGAIEETIETPERDE
                     SKNTVMHVLRSFDINKPGSKIKNLKGGVIGGSLTQGKFSVGDEIEIKPGLLDGRKKSY
                     QSIQTEIVSLGTGAGLVDSVKPGGLVAIGTKLDPSLSRSDSLIGSIIGKPDTLPENTT
                     NVKLSINLFDMAVGSVTETKVAPIRTDELLRVNVGTAPVPAKVKKVKSNDIEVEFKRP
                     ICLFEGSNVALSRRIADRWRLIGAGIVG"
     gene            1481..1849
                     /locus_tag="QGF01_01755"
     CDS             1481..1849
                     /locus_tag="QGF01_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HJN19657.1"
                     /translation="MVDVICDTSFLIHLATRNIKNLDSIYSEIGNIEFVVPLVVLNEL
                     EQLTKNQNKQDDASKTLEFVRDMKNIEILGKFADNAISEYIQKHGGMVATMDNELKNK
                     IKNLNGTIISFSNDKIVLES"
     gene            1886..2332
                     /locus_tag="QGF01_01760"
     CDS             1886..2332
                     /locus_tag="QGF01_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HJN19658.1"
                     /translation="MGDLQLTSSAFTDGGEIPRECGYKNGNEVPPLTISGKPEGTKSL
                     ALIMDDPDAMGAVGKVWVHWVIWNINPDTGQVDGYIDDPEGVTDFGERGYGGPAPPDK
                     RHTYVFKLYALDTRLDLPDKSTKADLEKAMEGHILEQTQLTGTYAP"
     gene            complement(2339..2509)
                     /locus_tag="QGF01_01765"
     CDS             complement(2339..2509)
                     /locus_tag="QGF01_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HJN19659.1"
                     /translation="MRFQLRKCSVCNRYTLKNECLKCNNPTKLVHPAKFSPDDKYMRL
                     RMEKNSNLDSTK"
     gene            complement(2509..3303)
                     /locus_tag="QGF01_01770"
     CDS             complement(2509..3303)
                     /locus_tag="QGF01_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HJN19660.1"
                     /translation="MSTEMQEFPDEGEIVVVTVTKIGDHGAYVGLDEYNSIQGFLHIS
                     EIAPGWVRNVRKYVTEGEKKVVLVKKIRKAKGEIDLSLRQISKDQQKKKLLDVKRHEK
                     GKGIIKNIQDKAKLSEKQIEEVEDKIFSKYDSVYDAVIDIGTNGIKVFDTFGLPRETL
                     DVIEEVSSKIKLPSVEIRGVLEISDSSFNGIENIKKQLQDAEQKGGVEILYIGAPKYR
                     MSVRAPDFKSAERTLKPILEDLQKNIEKNKGTFVFTREESKKTRGD"
     gene            complement(3343..3909)
                     /gene="cobO"
                     /locus_tag="QGF01_01775"
     CDS             complement(3343..3909)
                     /gene="cobO"
                     /locus_tag="QGF01_01775"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HJN19661.1"
                     /translation="MGEKGLVIVYTGGGKGKTSAALGLVLRAVGYNHKVCMIQFVKGS
                     WHYGELDSAKRLEPEFELVTAGKGFVGIIDDKSPREDHVKAANDALTISKEKILSGKY
                     DVVILDEINYAIQLELVSLNDVLEIIKSKPDELDLVLTGNLCPLGKGDVQSTAAEKII
                     EVADLVTEMKEVKHPFKSGIKAKKGIDF"
     gene            3972..5306
                     /locus_tag="QGF01_01780"
     CDS             3972..5306
                     /locus_tag="QGF01_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HJN19662.1"
                     /translation="MKIPRLIIAGATSGVGKTTITSAIIYGIKKCGYTVQPFKVGPDY
                     IDPSYLSSISKNNAKNLDVWLMGKNELVQSFVKNSTSDISIVEGVMGYYDGFGGKTNF
                     ASTHHVASLLKAPTVLVLDASKAARSIAATALGFAKFHRNSRIVGIILNRIGSKKHEN
                     MCRQALTNLKIPILGFIPKNSESMESRHLGLIPVREQKSLQNKIRKVSREISDNLDIN
                     KIIQICKSVPELPKMKPKKFKKSIATVAVALDSSFNFYYHDNLEALRREGAKLKFFSP
                     INDKKIPACDLIYIGGGFPEVLGQSLERNTTMRKMIKKRAEEGTSIYAECGGLMYLTK
                     LISFGKKKYKMVGLFDAETHMTKKMTLNYTEGRIRSNCFVSPTAKFRAHEFHYSKIRN
                     LPRDAKLVYDLKIGEGISGKKDALSEYNTLASYCHLYFDSAKYATNMVRNSI"
     gene            complement(5320..5562)
                     /locus_tag="QGF01_01785"
     CDS             complement(5320..5562)
                     /locus_tag="QGF01_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HJN19663.1"
                     /translation="MTEPEIPQKRPYPTQVEAGKEYHVCMCGKSQNQPLCDGSHAGSG
                     LGPKIFKATEDGFIAFCGCKHSNDESGKCDGTHGTL"
     gene            complement(5613..6242)
                     /locus_tag="QGF01_01790"
     CDS             complement(5613..6242)
                     /locus_tag="QGF01_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HJN19664.1"
                     /translation="MQTRKGQNIEDESMQVIDNEVGPHSYDELEWPIVRRMIHSTADF
                     DFAGKNKIIFHKDAISSGMSALKNGCSIICDVNGLVGFLNKQNPKDFGNEVICNISNP
                     IVIETAKKDGKTRAEASMRFVASEMNGGIVAIGSAPTALYEVLKMIKEGVTKPALIIG
                     IPVGFIAAAESKDELCSSNIPFITNIGRKGGSSCAASTINALFKLIRQS"
     gene            complement(6232..6984)
                     /locus_tag="QGF01_01795"
     CDS             complement(6232..6984)
                     /locus_tag="QGF01_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HJN19665.1"
                     /translation="MKRGLMLIDRGSREREAEEELETICEKVKEKGNYDFTEFCFLEV
                     VPPFIEDGMKKCLKKDIDEMTIVPYFLYPGKKVKIAVADAMKYQKDTKIKFLVSKPMT
                     MHRTLVDLVDNRIVSALKENEISLSKDEVDVLVIGHGSKDPNAGMSINYIVDELRKTY
                     KNVDRCYLEIEEPDIEQGIQKCEKNKPKVLVIVFYFLHEGAHVKTDINNDLKPALEKV
                     NLNKVCITKHLGTDEKMIDLILERAKEVEDAN"
     gene            complement(6981..8039)
                     /locus_tag="QGF01_01800"
     CDS             complement(6981..8039)
                     /locus_tag="QGF01_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HJN19666.1"
                     /translation="MERIAIIAITKNGIKMAKGLKEKFPTWEIFAPEKFSDDDKKINW
                     YNNSTTIKIKELFESNDGLICLFSLGAVVRLISPHLKDKKTDPAVIVIDDQAQFVIST
                     LSGHLGGANQLTNDIAEQLSAVPVITTAADVNKTISVDLVGKDFGWKIDDDSNVTKIS
                     AFMVNKEKIAVYQSCGARNWWQQNLPENVSSYSTLDELKKSKSKGFLIISDEHIDDND
                     ILENAVIYRPPSLVVGIGLHWNTTKEKIMEGLDASLKKFNLSKKSIVRLASTKKREDA
                     VGLNDFTKEMNVPLELYDRDDLAPIKTPNPSKMVESYEGTASVSEAAAIKSSGGVLIV
                     EKQKFPPDLTIAIARIQE"
     gene            complement(8072..9169)
                     /locus_tag="QGF01_01805"
     CDS             complement(8072..9169)
                     /locus_tag="QGF01_01805"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HJN19667.1"
                     /translation="MDLPKQPTGKLRTGFTTGTSATAASIAGILSILNQEKIKSVDVL
                     LPKKDKIKININSCEFGKNHARCSVIKDGGDDPDVTHGAEIVVDLELTSNPNSVEIDG
                     GEGVGRITKPGIGLEIGQAAINPTPRKMITENLILTAKEILEKNGIKVLISVPKGKEL
                     GPKTDNPRIGIVGGISILGTSGIVMPYSTASFAAAIRQQISVVVSMGDDTVVLTTGGR
                     SEDYARKILEFPEHSYIQMGDFSGYTLSQCAKKDIKKAYVCGFIGKFAKMATGVKQTH
                     VKGSKVNMQFLSEIAKKCKAEQTIIKEIKNANTARNVQEIILENNIEGFFDEICSQVY
                     KQLTNHSENKTPIEIILFDFDGNVLARYPKQ"
     gene            complement(9205..9627)
                     /locus_tag="QGF01_01810"
     CDS             complement(9205..9627)
                     /locus_tag="QGF01_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HJN19668.1"
                     /translation="MLDSSRYYEILGINENSSSKEMKNAYRKLSLKYHPDKTNIDQSD
                     TKFKEVTEAYQFLKSHQKRVKKTIKNDVGSNYADFWQKQYEEQYEHGFNHRENFRNEF
                     HIKSPNQEKPVSHKIFHITIFGGLALITLWIVLSEILK"
     gene            9674..10528
                     /locus_tag="QGF01_01815"
     CDS             9674..10528
                     /locus_tag="QGF01_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HJN19669.1"
                     /translation="MTKQEISTITITGANGFVAKNLRNFLSKNHIKVIAIARKTFQKH
                     HTETAVYSKTLLEKGLQNKLRNCDALVHLIGIGKQSSKYNFEGNVDLTKNMIKTCKKS
                     GIKKIIYISGLGVTKNSRSDYFISKYKAEQEIINSGLNYTIFRPSYIVGKKDYLSKFV
                     LKQIKKGIVIIPGSGKYHLQPIFVEDVAKIILESISEKKFSNKILDLVGPEIIKFEDF
                     VRYFVKNKKIRIQKINLESIYHEALHNPKSIYDLDSLNLLIGDYTGNLKQLQKISNVK
                     LRPIKDIL"
     gene            complement(10523..>11178)
                     /gene="metK"
                     /locus_tag="QGF01_01820"
     CDS             complement(10523..>11178)
                     /gene="metK"
                     /locus_tag="QGF01_01820"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HJN19670.1"
                     /translation="QVSVEYVDNKPQRVQTVVISTQHDPEISNEEIRKEIIDKVIKPV
                     CGNWWHEEIVIHVNPTGKFEIGGPHGDAGLTGRKIIVDSYGGMGRHGGGAFSGKDPSK
                     VDRSACYMCRYVAKNLVAAGLADRCEVQIAYAIGVCQPLSLMVNTFETNKIPEEKIEK
                     ILNSHFDMKPASIVSHLDLKRPIYKKTAGYGHFGRNDPEFSWEKTDKAEDLRKAAGL"
BASE COUNT         3587 a         2088 c         1747 g         3756 t
ORIGIN      
        1 aaccaaagaa agaagctcct aaagaagaac caaagaaaga agctcctaaa gaagaaccaa
       61 agaaagaagc tcctaaagaa gaaccaaaga aagaagctcc taaagaagct cctaaagaag
      121 aaccaaagaa agaagctcct aaagaagctc ctaaagaagc tccaaagaaa gaacctaagg
      181 aaaaaaaggc atagattaac atattacgat ttacaatttt gcttaatgca ctggaaagac
      241 acacttcctg attggtacat aaaaaaatat ggaaagcaac catgcgtcaa tattggtacg
      301 gcaggtcatg tagatcatgg aaaaactact ctaattcagg ctcttactgg gtcttggaca
      361 agcgtacata gccaagaact caaacgaggt atcaccatac gtgttggtta ttcagacgca
      421 gcattctaca aatgtaaaaa atgtgaatca ccactaggtt attctaccga gcccaaatgt
      481 ccaaactgtg gaaaagaaag cgaattatca agagtggtaa gctttgttga tagtcctggg
      541 catgaaagtt tgatggctaa catgctttca ggttctgcat taattgatgg tgcaatgctt
      601 ttggttgctg caaactcaaa ggtaccacaa cctcaaacaa aagaacatct tttagccttg
      661 caaacattag gcattaaaca aattgtaatt gttcaaaata aggttgactt gatatcatac
      721 caagatgctt tatcaaattt ttctgaaatc actaaatttg tcaagggtac aaaacatgca
      781 gattcaccaa taattccggt atcagcacaa tcatctctta atgttgatgc gttgattgga
      841 gctattgagg aaacaattga aactccagaa agagatgaat ccaaaaatac tgtgatgcat
      901 gttctaagat cttttgatat caataaacca ggttcaaaaa taaaaaatct caaaggtgga
      961 gtcattggag gcagtctaac tcagggtaaa ttttcagttg gagatgaaat cgagattaaa
     1021 ccaggattat tagatggtag gaaaaaatct tatcaatcca tacaaacgga gatagtttca
     1081 ttgggtacag gagctggttt ggtagattct gttaaacccg gaggtttggt tgctatagga
     1141 acaaaacttg atccttctct ttcaagaagt gattcattga taggttcaat aattggaaag
     1201 ccggatacac tcccagaaaa tacaacaaac gtaaaactaa gcataaattt gtttgatatg
     1261 gctgttggat ctgttacaga aacaaaagta gcaccgatca gaactgatga attgttaaga
     1321 gttaatgtag gaacagcgcc agtacctgca aaagtaaaga aagtaaaatc caatgatatt
     1381 gaagtggaat ttaaaagacc aatctgcctt tttgagggat caaatgttgc acttagtaga
     1441 agaattgcag atagatggag actcattggg gcaggaatag ttggttgatg ttatctgtga
     1501 tacaagtttt ctaattcatc tagccactag aaatatcaaa aatcttgatt ccatatattc
     1561 tgaaattggt aatattgaat ttgtagttcc tttagtggta ttaaatgaat tagaacaact
     1621 tacaaaaaac caaaacaaac aagatgacgc atcaaaaaca cttgaatttg tgcgtgatat
     1681 gaaaaatatc gagatattag gtaaatttgc tgacaatgca atttccgaat atatccaaaa
     1741 acatggagga atggttgcaa caatggacaa cgagctgaaa aataaaataa aaaatctaaa
     1801 tggtacaatt atatcatttt caaacgacaa aattgttttg gaatcctagt aaaatttaac
     1861 agcaatataa tacaaaatta agcaaatggg agaccttcaa cttacaagtt ctgctttcac
     1921 tgatggtggt gaaataccac gagaatgtgg atataagaac ggaaatgaag tacctccatt
     1981 aacaatcagt ggaaaacctg agggaacaaa atccttagct ctcattatgg atgatccaga
     2041 tgcaatggga gctgttggaa aggtttgggt gcattgggtt atttggaata ttaatcccga
     2101 cacaggacaa gtagatggat atattgatga cccagaaggt gtgacagatt ttggtgaacg
     2161 aggatatggt ggaccagcac cgcctgacaa aaggcacaca tatgtcttca aactttacgc
     2221 attagatacc agactggatc ttccagacaa gtctacaaag gcagatcttg aaaaagcaat
     2281 ggaaggtcac atcttagaac agacacaact aacaggcaca tacgcaccct gattaagatt
     2341 atttagtaga atccaaatta ctgttttttt ccatccgcaa tctcatatac ttgtcatcag
     2401 gagaaaattt tgcaggatgt actaacttgg taggattatt acattttagg cattcatttt
     2461 ttagtgtata tctattacac acagaacatt ttcttaattg aaatcgcatt aatctcccct
     2521 agttttttta gattcttctc ttgtaaatac aaaagttccc ttattttttt caatgttttt
     2581 ttgcaaatct tctaaaattg gttttaatgt tctttctgca gatttaaagt ctggtgctct
     2641 cacactcatt ctatattttg gagcaccgat gtataatatt tctacaccac ctttctgttc
     2701 tgcatcttgc aattgcttct ttatattttc aattccatta aaactgctat cagatatttc
     2761 taatacacct ctgatttcta ctgagggaag tttaattttg gatgaaactt cttcaattac
     2821 atctaatgtt tctcgtggta aaccaaatgt atcgaaaact ttgattccat ttgtaccaat
     2881 atctattact gcatcataaa cggaatcata ttttgaaaaa attttatctt ctacttcttc
     2941 aatttgtttc tctgataact ttgccttgtc ttgtatattt ttaataatcc ctttcccttt
     3001 ttcatgtcgc ttgacatcaa ggagcttctt tttttgttga tcctttgaaa tctgtcttaa
     3061 tgataaatca atttctccct tagcttttct aatcttcttt acaagtacaa cttttttctc
     3121 tccttccgtt acatatttcc gaacatttcg tacccatcca ggagcaattt ctgaaatatg
     3181 taaaaagccc tgaatgctat tatactcatc gaggccgaca taagccccat ggtcacctat
     3241 ctttgtgact gttacaacta caatttcacc ctcatcgggg aattcttgca tctcagttga
     3301 cattcattct aattttttta ctagctaatt tattgttgct gatcaaaaat ctattccttt
     3361 ttttgctttt ataccagatt tgaacgggtg ttttacctcc ttcatctcag ttacaaggtc
     3421 agctacttca attatttttt cagctgcagt actttgaaca tctcccttac ctaatggaca
     3481 tagattaccc gtaagtacaa gatcaagttc atcaggtttt gattttataa tttctaaaac
     3541 atcgttcagg cttactaatt ctaattgaat tgcatagtta atctcatcta atattacaac
     3601 atcatatttg ccagacaaaa ttttctcttt actaatagta agtgcatcat ttgctgcttt
     3661 cacatgatct tcccgaggac ttttatcatc tattattcct acaaatccct tacctgcagt
     3721 aaccaactca aattctggtt ccaaccgttt agcactatcc aattcaccat aatgccatga
     3781 gcctttgaca aactggatca tgcaaacctt gtggttatag cctactgccc taagaacaag
     3841 acccaatgca gctgaagttt tccccttacc acctccagta tagacaatta ctaggccttt
     3901 ttcacccata tctagtttga attttatcta actaaaaaga ttgatactag atgaaattca
     3961 taggttaaga attgaaaatt ccaagattaa tcattgctgg agcaactagc ggtgtgggca
     4021 aaacaacgat cacatccgca ataatttatg gtattaaaaa atgtggttac acagttcagc
     4081 catttaaggt tggaccagat tatattgatc ctagctatct atcttcaata tcaaaaaaca
     4141 atgcaaaaaa tttggacgtt tggttgatgg gcaaaaatga gcttgtccaa agttttgtaa
     4201 aaaattctac atctgacatt tctatagttg agggtgttat gggttattat gatggatttg
     4261 gtggaaagac aaactttgca agtacacacc acgttgcgtc actactaaaa gctccaacag
     4321 ttttagtttt agatgcaagt aaagctgcgc gttctattgc agcaactgcg cttgggtttg
     4381 caaagtttca tcgtaattct aggatagttg gaataatttt gaacagaatt ggcagcaaga
     4441 aacatgaaaa catgtgcaga caggctctca caaatctaaa aattcccata cttggattta
     4501 tcccaaaaaa ttctgagagt atggaatcac gacatttagg tctcattcct gtaagggaac
     4561 aaaagtcact gcaaaacaag attagaaaag tatcacgcga gatttctgat aatcttgata
     4621 ttaacaaaat aattcagatt tgtaaaagtg tgccagaact gccaaagatg aagccaaaaa
     4681 aattcaaaaa atcaatagct acggtagcag tagcactaga cagttcgttt aatttttact
     4741 atcatgataa tcttgaagca ttacgtagag aaggtgcaaa actaaaattt tttagcccta
     4801 taaatgacaa aaaaattcca gcctgtgatt taatctacat tggtggtgga tttcctgaag
     4861 ttttggggca atcacttgaa aggaacacaa caatgaggaa gatgataaaa aaacgtgctg
     4921 aagaaggaac gtcaatttat gccgaatgtg gagggttgat gtatcttact aaattgataa
     4981 gctttgggaa aaaaaagtac aagatggtag gattgttcga cgcagaaact catatgacaa
     5041 aaaaaatgac attaaactat actgaaggga gaatcagatc aaattgtttt gtttcaccaa
     5101 ctgcaaagtt tcgtgcacat gagtttcact actcgaagat tagaaatcta ccaagagatg
     5161 caaagcttgt ctatgatctg aagattggtg aaggcatatc tggtaaaaag gatgcacttt
     5221 ctgaatataa cacacttgca tcatactgtc atctgtattt tgatagtgca aaatatgcta
     5281 caaacatggt tcgaaattca atctagcaat ttagagagat taaagtgtgc catgtgttcc
     5341 gtcacacttt ccactttcat cattactatg tttgcatcca caaaaagcaa taaatccatc
     5401 ttcagtagcc ttaaaaattt ttggtcctaa accggaacct gcatggctac catcacaaag
     5461 tggctgattt tgggattttc cacacataca tacatgatat tcttttcctg cttcaacttg
     5521 cgtaggatat ggtctttttt gtggtatctc tggttcggtc ataattttta ttaaccgccg
     5581 tacttgtaag tataactaaa tttttgatcg gcttaactct gtcttattaa tttgaataat
     5641 gcatttatgg tagacgcagc gcaagaactt ccaccttttc ttccaatatt tgtgataaac
     5701 ggaatattag agctgcacaa ttcatcttta gactcagctg ctgcaataaa acctacagga
     5761 ataccaataa ttagagcagg ttttgtaaca ccttccttaa tcatcttaag aacttcatac
     5821 agagcggttg gggcacttcc aatagccact attcctccat tcatttcaga ggcaacaaaa
     5881 cgcatagatg cctctgccct tgtcttgcca tcttttttag cagtttcgat cacaattgga
     5941 ttagaaatat tgcaaatcac ttcatttcca aaatcttttg gattttgttt attgagaaaa
     6001 ccaacaagac cattaacatc acaaattatg ctacacccat tctttaacgc agacattcca
     6061 ctagaaattg catctttgtg gaaaataatc ttattttttc cagcaaagtc gaaatcagct
     6121 gtagaatgta tcatacgtct tactatgggc cattctaact catcatatga atggggaccc
     6181 acttcattat caataacttg catactctca tcctcaatat tctgaccttt cctagtttgc
     6241 atcttctacc tcttttgctc gctctaaaat caaatcaatc attttttcat cagttccaag
     6301 atgcttagta atacaaactt tgtttagatt gactttttct agcgcaggtt tcagatcatt
     6361 gttaatgtct gtcttcacat gagctccttc atgcagaaaa taaaagacta tgaccaaaac
     6421 ttttggcttg tttttttcac atttttgtat tccctgttca atgtctggct cttcaatctc
     6481 taagtagcac ctgtccacat ttttgtaggt ttttcttagc tcatctacaa tgtaattaat
     6541 tgacattccg gcattaggat ctttactgcc atgaccaatt acaagcacat ctacctcatc
     6601 ttttgataat gagatctcat tttcctttaa tgctgaaact atcctattat ccaccagatc
     6661 tactaatgtt ctatgcatag tcattggttt tgagacaaga aacttgattt tcgtatcttt
     6721 ttggtacttc attgcgtctg ccaccgctat ctttactttt tttccaggat atagaaaata
     6781 cggtacaatt gtcatttcat caatatcttt tttcagacat tttttcatcc catcttcaat
     6841 gaatggtgga actacctcaa gaaaacaaaa ttctgtaaaa tcataatttc ctttttcctt
     6901 aactttttca caaatagttt ctaattcttc ttcagcctct ctttccctac tacccctatc
     6961 gattaacatt aatccacgtt tcattcttga atcctcgcta ttgctattgt taaatctggt
     7021 ggaaattttt gtttttcaac aatcaaaact ccacctgatg attttatagc tgcagcctca
     7081 gacacactag ctgttccttc ataactctca accattttag atggattagg agttttgata
     7141 ggcgcaagat catctctatc ataaagctcc aatggtacat tcatttcttt tgtaaaatca
     7201 ttcagtccta cagcgtcctc tcttttcttt gtagacgcaa gtctgactat ggatttttta
     7261 cttagattga attttttcaa agatgcatcc aacccttcca ttatcttctc ttttgtagtg
     7321 ttccagtgta aacctattcc tacaactaaa ctaggaggtc tgtaaataac tgcattttct
     7381 aatatatcat tgtcatcaat gtgttcgtcc gaaataatca agaagccttt tgattttgat
     7441 ttttttaatt catcaagtgt tgaatatgat gaaacatttt ctggtaagtt ttgttgccac
     7501 caatttcgtg caccacaact ttgatacaca gctatttttt ctttgtttac cataaatgcg
     7561 ctaatcttag ttacattaga gtcatcatca attttccatc caaaatcttt tccaacaagg
     7621 tctaccgaaa ttgttttgtt gacatcagct gctgttgtta ttacaggtac tgcactaagt
     7681 tgctctgcaa tatcatttgt aagctgattt gctccaccaa gatgtccaga aagtgtactt
     7741 atgacaaatt gagcttggtc atcaattaca attactgcag gatccgtctt cttatccttc
     7801 agatgtggtg aaattaatct aaccactgca cccaatgaga ataaacaaat caacccatca
     7861 tttgattcaa ataattcctt aatttttatt gtagtgctat tgttatacca attaattttt
     7921 ttatcatcat ctgaaaattt ctccggggca aatatttccc acgtgggaaa tttttccttt
     7981 aagcctttgg ccatttttat gccatttttt gtgatagcta tgatcgctat tctttccata
     8041 tcctagttgt ttcttagttt aataaaatcc cttactgctt tggatatcgt gccaaaacgt
     8101 tcccatcaaa gtcaaatagt atgatttcga tgggagtctt attttctgaa tgatttgtaa
     8161 gctgtttgta aacttgtgaa cagatttcat caaaaaaacc ctctatatta ttttcaagaa
     8221 taatttcttg tacatttcta gcggtatttg cattctttat ttccttgatt atagtttgtt
     8281 cagccttaca cttctttgct atttctgaga gaaattgcat attcaccttg gagcctttta
     8341 catgtgtctg cttcacacca gtagccattt ttgcaaattt tccaataaaa ccacaaacat
     8401 aagctttctt gatatctttc ttcgcacatt gagaaagtgt gtatccagaa aaatctccca
     8461 tttgtatgta tgagtgttct ggaaattcaa gtatcttcct tgcataatct tcacttcttc
     8521 caccagttgt caagacaact gtatcatctc ccattgaaac tacaacacta atttgttgtc
     8581 gaatagcagc agcaaatgat gcggtagaat atggcataac aattccacta gttcctagta
     8641 ttgatattcc tccaactatt ccaattctgg ggttgtcagt ttttggcccc aactcctttc
     8701 ccttcggaac tgatatcaat actttaattc catttttttc tagaatttct ttggcagtta
     8761 gaattaaatt ttcagtaatc atttttcttg gagttgggtt tattgcagct tgaccaattt
     8821 ccagtcctat accaggcttt gtaatacgac caacaccttc tccaccatca atttcaaccg
     8881 agtttggatt agatgttagt tctaaatcca caacaatttc agcaccatgt gttacatcag
     8941 gatcatcacc accatcctta atcactgaac agcgtgcatg attttttcca aactcacaac
     9001 tattgatatt tatttttatt ttatcctttt ttggtaataa cacatctaca gatttgattt
     9061 tttcttgatt aagaattgat aggataccag ctattgatgc ggcggtggca ctagtaccag
     9121 tggtaaatcc tgttcttagc ttacctgtag gttgttttgg taagtccaca ttataattga
     9181 gatttttttt atgtaataac tcttttattt caaaatttca gataaaacaa tccagagtgt
     9241 aatcaatgca agaccaccaa aaatagtaat atgaaaaatt ttatgtgaaa ctggtttttc
     9301 ctgattagga gattttatgt gaaattcatt cctaaaattt tctctgtgat taaaaccgtg
     9361 ctcgtattgt tcttcatatt gtttttgcca aaaatccgca taattacttc caacatcatt
     9421 tttgatggtt tttttcactc gtttttgatg tgatttgaga aattgatatg cttctgtaac
     9481 ttctttgaat ttagtgtctg attgatcaat attggtttta tctggatgat attttaacga
     9541 cagttttctg tatgcattct tcatttcctt tgatgacgaa ttttcattta tacccaatat
     9601 ttcataatat ctcgaagaat caagcacaaa ttgtatatga tatctttaaa attaaatctt
     9661 gtatgatttt tttatgacta aacaggaaat ttcgactatt actattacgg gcgcaaatgg
     9721 ttttgtcgca aaaaacttgc gaaattttct atctaaaaat catattaaag tcatcgcaat
     9781 tgctagaaaa accttccaga aacatcatac tgaaacagcg gtgtactcaa aaactcttct
     9841 ggaaaaagga ctccagaaca aattaagaaa ttgtgatgct ttagtacatc ttattggaat
     9901 tggcaaacaa tcctccaaat ataattttga aggtaatgtt gatctaacaa aaaatatgat
     9961 taaaacatgt aaaaaatctg gaattaaaaa aattatttat atcagtggtt tgggcgtaac
    10021 taaaaattct agatcggatt attttatctc aaaatacaaa gctgaacaag aaataattaa
    10081 ttccggtctg aactatacaa tatttcgacc ctcttatatt gtagggaaaa aagattatct
    10141 aagtaagttc gtcctgaaac aaattaaaaa aggaattgtg ataattcctg gttctggtaa
    10201 atatcattta caaccaatat ttgtagaaga tgttgctaaa attatactcg aatcaatttc
    10261 tgagaaaaaa ttttctaata agatattaga tttagtggga ccagaaatca taaaatttga
    10321 agattttgtt agatattttg ttaaaaataa aaaaataagg atacaaaaaa ttaatttaga
    10381 aagtatatat catgaagcat tacataatcc aaaaagcatc tatgatttag atagtctcaa
    10441 tcttttgatt ggagattata caggcaatct aaagcaactt caaaaaatat ccaatgtaaa
    10501 actaagacca attaaagata ttttataatc cagcagcctt tcttagatct tcagctttgt
    10561 ccgtcttttc ccaactaaac tctggatcgt ttctaccaaa atgaccataa cctgcggtct
    10621 ttttgtagat tggtcttttt agatctaaat gagaaactat cgaggctggc ttcatatcaa
    10681 agtgtgagtt tagaattttc tcaattttct cttcaggtat tttatttgtt tcaaacgtat
    10741 taaccatcaa tgataatggt tgacaaactc ctatagcata agcaatctgc acttcacatc
    10801 tatcagcaag acctgccgcc acaagatttt ttgcaacata tctgcacata taacatgcag
    10861 atcgatctac ctttgatgga tcctttccag aaaatgcccc accaccatgt cttcccattc
    10921 ccccatagga atcaacaatg atttttctgc ccgtaagtcc agcatcaccg tgtggtcctc
    10981 caatttcaaa tttcccagtt gggtttacat gaatcacaat ctcttcatgc caccaatttc
    11041 cacaaacagg ttttatcact ttatcaataa tctcctttct aatttcctca tttgatattt
    11101 caggatcatg ttgagttgaa attacaacag tttgaactct ttgaggctta ttatctacat
    11161 attcaacaga aacttgag
//
LOCUS       DASCPJ010000035        11061 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000649621, whole genome shotgun sequence.
ACCESSION   DASCPJ010000035 DASCPJ010000000
VERSION     DASCPJ010000035.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000649621"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="QGF01_01825"
     CDS             <1..1398
                     /locus_tag="QGF01_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HJN19671.1"
                     /translation="SFMFRYSKLSGISKFQEFALKTLKKMAKGGIFDQIGGGFHRYST
                     DTRWLVPHFEKMLYDNALLPIVYSEAYQITKDPFFENVVRKTLDYVIREMTSNEGAFF
                     SAQDADTNGEEGQTFVWKKQEIEKILGEDTEIFCIYYDVTDGGNFEGNTILANNINAS
                     SLAFKFGKNESEIRAIISKCSDKLLEVRNKREQPGKDDKIITSWNGLMISAFLSGYKI
                     TDNIKYFDIGKASIDFFENNFEKNHILYRTFKNGKSKLNGYLDDYAYMANASIDMFEA
                     TSQPKYLVFAINLANYLLTHFWGDSINGFFFTSDNHEKLIIRPKNNYDLSMPSGNSVA
                     AHMLLRLHHITQNEQFLEISKKIIESHVISAAENPFAFGYLLNVSYLYYQKPTEITII
                     NDKNPKIISYLRKKFLPESIMVIVPNQNNLNVLSKYPFFSGKEFQDNETNVFVCKNFS
                     CSLPLSDLSEIEKEL"
     gene            complement(1395..1715)
                     /locus_tag="QGF01_01830"
     CDS             complement(1395..1715)
                     /locus_tag="QGF01_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19672.1"
                     /translation="MSDIIGYKIQQIIDNGPYVQITLTEDVQLEPISQKQLVMESVSK
                     KLDQETKQQVMPLLEAILQSQPTIKIKNYQHTSIAITMPKNRYDKMGRPQVGEKLQVD
                     LKKI"
     gene            complement(1773..2057)
                     /locus_tag="QGF01_01835"
     CDS             complement(1773..2057)
                     /locus_tag="QGF01_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19673.1"
                     /translation="MVISKENQEFLEGLIDYYVKEAKSYREIAQEFSSEINSITDTAF
                     GIIIGCIYSSFLQAYSNQKQVPDMEDIQEFNEMITKNTEIIKKSIMNENV"
     gene            complement(2051..2812)
                     /locus_tag="QGF01_01840"
     CDS             complement(2051..2812)
                     /locus_tag="QGF01_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HJN19674.1"
                     /translation="MKIVAVFIVLLIFVIPGVWAEVYIENDYQYISTDDTIHIAGEII
                     NDSNTPLNQVVVSAIIYSEENIIHEANTTTLTNLIMPGMKAVFDLQITEKIGTIDNYL
                     LDIDYKVAYPKNQVIEITSSEMKYGPSQNLIIQGTVANHGDATANMINVIATLYDKDG
                     NIVGVSKTSMEPDYLRANDESVFVIAILDGSESHEIVNYSLTAESEEYTAVPEFPFAS
                     GTLLMVSLSAYIILTKTPFQRTINYYKSHIFHNKW"
     gene            complement(2844..3353)
                     /locus_tag="QGF01_01845"
     CDS             complement(2844..3353)
                     /locus_tag="QGF01_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19675.1"
                     /translation="MKKRRGKKKGSLMYVAVIAGIVIIASSIFITYSNEQGTVRGKIF
                     GEELQIIQEDLKKLTHTFDSNVSMFKDGSLTKEGFLKLTKNHIKEMEELVLQYDKLSV
                     PNSFVSSVKLFKLSSESQLESDNQMIEWVETGDDAKRVRSDSLLQDSFEYEMAALAKY
                     KSAQGQLNP"
     gene            complement(3356..3643)
                     /locus_tag="QGF01_01850"
     CDS             complement(3356..3643)
                     /locus_tag="QGF01_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19676.1"
                     /translation="MEKIEYVGTVFVLDHKYPEPLVDHSLKKLQQLGIKKDDIKVTDA
                     PDNPKVGSVVVEIFPYHLDISRVRTIRNDSFISGTLMTLELSTDAEGNYID"
     gene            complement(3677..3910)
                     /locus_tag="QGF01_01855"
     CDS             complement(3677..3910)
                     /locus_tag="QGF01_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HJN19677.1"
                     /translation="MHKGFVLLNCDLGAEEYIIEELKQIPQVAHAYVTFGAYDVIVEI
                     DAASQETFDETVALKIRRLTRIVSTMTLNVVNS"
     gene            complement(3960..5252)
                     /locus_tag="QGF01_01860"
     CDS             complement(3960..5252)
                     /locus_tag="QGF01_01860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJN19678.1"
                     /translation="MTKFAKLGLEKSLLSVLDDIGFDTPFPIQERAIPVLLEGDDVIG
                     QAHTGTGKTAAYSLPMLQLVTPKAGIQGVIMAPTRELAIQITGEIKRFAKNIGIKTAT
                     IYGGQGMGIQFDALRKKPEILVATPGRLIDHLKRGTIKFNNIKHVVLDEADVMLDMGF
                     VDDIEFILDLTPEEKTVSLWSATMPPEIMRLAETNMISPKRILVDADDLSGAGIDQSF
                     LIIKDKEKMKFLFDFLDGNIGQCIVFCSTKMRTRTVARELYKARESVVTIEGDMSQHK
                     REDSMQRFRKSKADILVATDVASRGIDIPAVALVVNYDIPNQDMVYFHRIGRTARAGA
                     KGRAITLVSYSSVAEWEAIKKQTKVKMTDLNEKLGIKISIPDPLKRRSMPRQNFRQFR
                     KPMKQFTRRRQPSRDQYEKRTNKTEYVKRHNFNQKRRW"
     gene            complement(5382..5456)
                     /locus_tag="QGF01_01865"
     tRNA            complement(5382..5456)
                     /locus_tag="QGF01_01865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5419..5421),aa:Thr,seq:tgt)
     gene            5551..5772
                     /locus_tag="QGF01_01870"
     CDS             5551..5772
                     /locus_tag="QGF01_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19679.1"
                     /translation="MQLKYIPPKKLKVLIIMFFVAAAFGIFVGLVIAKGGQGFYITLL
                     GVVNLCLGGFMAYLLMTQKPKVRDSRKRK"
     gene            5822..6373
                     /locus_tag="QGF01_01875"
     CDS             5822..6373
                     /locus_tag="QGF01_01875"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HJN19680.1"
                     /translation="MLDLVAKKLFLTKGVGIADDKLTSFEFALRNAGISGTNIVLISS
                     IFPPRAKLIPRKEGLKLIKPGQILFTIYSRTQTNEPHRLISASVGVAQPKDRSKYGYL
                     SEYDAYGQNESKAGDYAEDIAAQMLASSLGIKFDLDKSWDEKRQQWTISGQIYKTKNI
                     TQSAKGDKNGKWTTVFAAAVLIV"
     gene            complement(6370..7887)
                     /locus_tag="QGF01_01880"
     CDS             complement(6370..7887)
                     /locus_tag="QGF01_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HJN19681.1"
                     /translation="MYKILLLATVALLFVFYIPQSFSESIEYSSWSETAQLVIDEKGG
                     KTSVSLGVMSKNPDDIMIPELLESMILNSPRLIGISYTNQYDCVPGVEEVVDYELHEF
                     SCVMIDVTRPSEIRGLVELKTDAQAVGDSFIGVINKSFGIDAEFHSVFINRMGLTSDL
                     YKSKLTVTYIIPKKPSVEHFDEITAPFVKDEIHNGGGFFKHAQKMAEHKDSVFSFSLV
                     PVDNRVLRSITVLMEDWGQYDITNMRISLLELLMVDTLERSKVFTDGFYPLNSIISLT
                     VIPDQNSQITELNSNLIIDPANIEELKTGGWYFDSSSTSELGEVISGKYLFGTESSVD
                     REDMYLIIGNNDGTEPVIHNDVIVEENASDNPSYDEAVKQATEFKERGGCLIATAAFG
                     SEMAPQVQFLREIRDNTVLKTESGTAFMTSFNQFYYSFSPTIADYERENPVFKEVVKL
                     TLTPLLTSLTLLQYADIDSESEMLGYGIGVILLNIGMYFIVPALVIIKIKTIKKI"
     gene            complement(8052..9824)
                     /locus_tag="QGF01_01885"
     CDS             complement(8052..9824)
                     /locus_tag="QGF01_01885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HJN19682.1"
                     /translation="MTEYKLSKWSLDDLLKNPTRSTFDKKLAELNNYAREFEKQKKFL
                     NKKISSKKLLELIHKIEHITEKSSIIGGYASLQYSENTQSDEATSLLTRISQFGSEIE
                     NRLLFFDLWWKKQIDEKNARRLIKSSGEYSQYLEFKRILAKYSLSEPEEKIINTLDVT
                     GASALVKLYDKITNAFVYVVKIDGKKKRMNREELTTLVRNKKSKVREAAYKSLFSKYS
                     ENKGVTGEIYQNIVLNLKDEGIGMRGFSSPIMIRNIANNVDEKTVKTLLDVCRRNAKV
                     FQKYFKEKAKLLGMKKIRRYDLYAPRKSNPKEKTYSYDKSVKLVLKSLDKFSPTLSKY
                     ASSVFSQNHVDSELRPGKVSGAFCSTPSPNKIPYVLMNFTGRSRDVFTLSHEIGHAIH
                     SICASKNSILISDASLPLAETASTFSEMLLYDNLSELVSDEERKIMLSEKIDDFYATI
                     GRQSFFTLFEISAHQQIANSTTVDEISKTYLHNLNEQFGSSVNVSEDFQTEWSCIPHF
                     YHAPFYCYSYAFGNLLSLSLFQKYKREGSGFASTYTEILSAGGSKKPEKLLQEFGMDI
                     TKSRFWQDGFDYIKTQVDELSRLG"
     gene            complement(9830..10087)
                     /locus_tag="QGF01_01890"
     CDS             complement(9830..10087)
                     /locus_tag="QGF01_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HJN19683.1"
                     /translation="MTDECEHFGEASNEVSPNTKGCEECEKEKLPWVAIRMCLTCGHV
                     GCCDSSVGLHATKHFKETGHPVMVAVPDKSWRWCYIHKDYY"
     gene            10166..10240
                     /locus_tag="QGF01_01895"
     tRNA            10166..10240
                     /locus_tag="QGF01_01895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10201..10203,aa:Val,seq:cac)
     gene            10272..10397
                     /locus_tag="QGF01_01900"
     CDS             10272..10397
                     /locus_tag="QGF01_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19684.1"
                     /translation="MDKRDWIIYKKKEIGWAIGIGIMTVGFLFLVNNFGLGMFRP"
     gene            10745..>11061
                     /locus_tag="QGF01_01905"
     CDS             10745..>11061
                     /locus_tag="QGF01_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJN19685.1"
                     /translation="MKVTKISAVTLPVSDMKKSVDFYSKIPNFKIVYGGPDSQFTSFL
                     IEDTTKSYLNLKLNEVHPPNFGRIIFYVDNVDDLFAYMESDETISGLGKLESKPEDAE
                     WGER"
BASE COUNT         3490 a         1935 c         1715 g         3921 t
ORIGIN      
        1 tcattcatgt ttagatactc aaaactttct ggaatatcca agttccaaga atttgcattg
       61 aaaactttaa aaaaaatggc taaaggtgga atctttgatc aaattggtgg cggttttcat
      121 cgttattcta ctgacacaag atggttagtg ccacattttg aaaaaatgtt gtatgataat
      181 gcactattac ctatagttta ttctgaagcg tatcagatta ccaaagatcc tttctttgag
      241 aacgttgtaa ggaaaactct tgattatgtc atccgtgaga tgacatcaaa tgaaggcgca
      301 tttttttctg cacaagatgc tgatacaaat ggggaagagg gtcaaacgtt tgtatggaaa
      361 aagcaggaaa ttgaaaaaat tttgggtgag gacactgaaa tcttctgtat ttattatgat
      421 gttaccgatg gaggtaattt cgagggtaat acaatccttg caaataacat taatgcatct
      481 tctttggctt tcaaatttgg aaagaatgaa tctgaaatac gggctataat ttcaaaatgt
      541 tctgacaaac tacttgaggt tagaaacaaa agggaacagc ctgggaaaga tgataagatt
      601 ataacttcat ggaatggctt gatgatttcc gcctttttat caggctataa aataactgat
      661 aacataaaat attttgatat cggaaaagcg tcaatagatt tctttgaaaa caattttgag
      721 aaaaatcata ttttgtatag aacattcaaa aatggcaagt cgaagctgaa tgggtattta
      781 gatgattatg catatatggc aaacgcatca attgatatgt ttgaagctac ttcacaacca
      841 aaatatttag tatttgcaat taatcttgct aactatctat taacacattt ttggggtgat
      901 tccattaatg gatttttttt cacatctgat aatcatgaaa aattgatcat acgtccaaaa
      961 aataattatg atctttcaat gccatctggt aattcagttg cggcacatat gttgcttagg
     1021 cttcatcata taacacaaaa tgagcaattt ttagaaatat ccaaaaaaat tatagaatca
     1081 catgtaatat ctgcagctga aaacccattc gcatttggat atctacttaa tgtgtcatat
     1141 ctctattatc agaaacccac tgaaattact ataatcaatg ataagaatcc caagattatt
     1201 tcatatttaa gaaaaaaatt ccttccagaa tcaattatgg tcatagtacc aaatcaaaat
     1261 aatttgaatg ttttatcgaa atacccattc ttttctggaa aagaatttca agataatgaa
     1321 acaaacgtat ttgtatgtaa aaattttagt tgttcattac ctctgtctga tttatctgag
     1381 atagaaaaag aactctaaat cttttttaaa tctacttgta atttttcccc aacttgaggt
     1441 ctacccattt tatcatatct attcttaggc attgttatag caattgatgt atgttggtag
     1501 ttttttattt tgattgttgg ttgtgattgt aatattgctt caagtaatgg cattacttgc
     1561 tgcttagttt cttgatcaag tttttttgaa acagattcca taacgagttg tttttgtgaa
     1621 ataggttcta actgaacatc ttccgttaat gtaatttgta catacggacc attatctatt
     1681 atttgttgta ttttgtatcc tatgatatca gacaataaga taactaaaac atgttggaat
     1741 ttatctctaa ctaattcttt attctaattt agttatacat tttcgttcat gatagatttt
     1801 tttataattt ccgtattttt tgttatcatc tcattgaact cttgaatatc ttccatatct
     1861 ggaacttgtt tttgatttga atatgcctga agaaaacttg aataaataca accgattatg
     1921 ataccaaagg cagtatctgt tattgaatta atttctgaac taaactcttg cgctatttct
     1981 ctatacgatt ttgcttcctt cacgtaataa tcaattaatc cttctaaaaa ttcctgattt
     2041 tcttttgaaa ttaccattta ttatgaaaga tgtgcgattt ataataatta attgttcttt
     2101 gaaatggagt tttagttaga atgatgtatg ccgatagtga caccattaga agtgtaccag
     2161 aagcaaaagg aaattctggc actgccgtat attcttctga ttctgcagta agcgaataat
     2221 ttacaatttc atgtgattct gatccatcca gaatagcaat aacaaaaact gattcatcat
     2281 ttgctctaag ataatctggt tccatactag tttttgatac accaacaata tttccatctt
     2341 tatcatacaa tgtagcaatc acatttatca tgttagcagt agcatcaccg tgatttgcaa
     2401 cagttccctg tattatcaga ttctgcgatg gcccatattt catttctgat gatgtaattt
     2461 caatcacttg gttttttgga tacgcaactt tgtaatcaat atctaaaaga taattatcta
     2521 ttgtacctat tttttcagtt atttgcaaat caaacaccgc tttcatccca ggcataatca
     2581 aatttgttaa tgtggtagta tttgcttcat gaataatatt ttcttctgaa taaataatag
     2641 cagatacaac aacttgattc aacggagtgt ttgaatcatt aattatttct ccagcgatat
     2701 gaattgtatc atcagtactg atgtattgat agtcattttc aatgtaaact tcagcccaga
     2761 cacctggtat tacaaatatc aataaaacaa taaacacagc tactattttc aatatacttc
     2821 attccttgat ataattaatg aagttatggg tttaattgac cttgagctga tttgtattta
     2881 gccaaagcag ccatctcata ttcaaacgaa tcttgtagca acgagtcaga tcttacacgc
     2941 tttgcatcat caccagtttc aacccactca atcatttggt tgtcgctttc taattgagat
     3001 tcagatgaca atttaaagag cttgacagat gatacaaaag agtttggtac tgatagttta
     3061 tcatattgaa gaacaagctc ttccatttct tttatatgat ttttagtcag tttcaagaat
     3121 ccttccttag tcaggctacc atctttgaac atggatacat tcgagtcaaa cgtatgagtt
     3181 agttttttta aatcctcttg gattatttgt aattcttccc caaatatttt acctctaaca
     3241 gttccctgct catttgaata tgtaatgaat atactagaag caattataac aattccagca
     3301 ataaccgcaa catacatcaa agagcctttc tttttacctc ttcttttttt caagattaat
     3361 ctatataatt cccttctgca tctgtagata gttctaacgt cattaatgta ccactaatga
     3421 aagaatcatt tctaatcgtt ctaacacgtg aaatatctaa atgataagga aatatttcaa
     3481 caactacact cccaacttta gggttatctg gagcatctgt cactttaata tcatcttttt
     3541 tgattcccag ttgttgcaat tttttaagac tgtgatcaac taatggttct ggatatttat
     3601 ggtctaacac gaatacagtt cctacatact caattttttc caattagaat aagcgttaag
     3661 ttatggcata taatcattag gaattaacca cattaagcgt cattgtacta acaatacggg
     3721 ttaaccgtct aatcttcaaa gcaaccgttt catcaaaagt ttcttgtgaa gctgcatcaa
     3781 tttccacaat cacgtcataa gctccaaatg taacatacgc atgagcaacc tgtggtatct
     3841 gctttaattc ttcaataatg tactcctcag cacccaaatc acaattcaat aggacaaatc
     3901 ctttatgcat attctattaa cttaatttat tatgttggct tggctttaaa tcttctttat
     3961 taccaacgtc ttttttgatt gaaattatgt cgtttaacat attctgtttt gttggttctt
     4021 ttttcatatt gatcacgtga aggctgtcgt cttctcgtaa attgtttcat aggttttcta
     4081 aattgacgga aattctgtcg tggcattgat ctccttttta atggatctgg tatgctaatc
     4141 tttattccaa gcttttcatt caaatctgtc atctttactt ttgtctgctt tttgattgcc
     4201 tcccattcag caactgaaga gtaagaaact aaagttattg ctctaccttt tgcaccagca
     4261 cgagctgttc ttcctattct atggaaatat accatatctt gattaggaat atcataattt
     4321 actacaagtg ccactgcagg aatatcaatt cccctagatg ctacatctgt agcaactaga
     4381 atatcagctt tagattttct gaacctttgc atagaatctt ctcgtttatg ttgagacata
     4441 tcaccctcaa ttgtaaccac actttcacgt gctttgtaca attcccttgc cactgttcta
     4501 gtccgcattt ttgttgaaca aaatacaata cattgcccaa tattaccatc aagaaaatca
     4561 aacaagaatt tcattttttc tttatctttt attatcaaaa atgattgatc aattccagca
     4621 ccactaagat catctgcatc aacgagaatt ctttttggag aaatcatgtt agtttcagcc
     4681 aaccgcatga tttcaggtgg cattgtagca gaccataatg atacagtttt ttcttctggt
     4741 gttagatcta aaataaactc gatatcatca acaaaaccca tatctagcat tacatcagct
     4801 tcatctagaa caacatgttt tatgttattg aattttatag ttcctctttt caaatgatca
     4861 attagtctac ctggagtagc aactaatatt tccggttttt ttcttaatgc atcaaactgt
     4921 attcccatac cttgtcctcc ataaattgtt gcagttttga ttcctatgtt tttagcaaat
     4981 cgtttaattt cacctgttat ttgtattgcc agttctctag ttggtgccat aataacaccc
     5041 tgtattcctg cttttggagt taccaattgt aacataggaa gtgaatatgc agcagtttta
     5101 ccagtaccag tatgtgcttg accaataaca tcatcaccct ctaataaaac aggaattgca
     5161 cgctcttgta tcggaaatgg tgtatcgaac ccaatatcat caagcacact caatagtgat
     5221 ttttctagtc ctaattttgc gaattttgtc attttatttt ttagcaataa cggagaacgc
     5281 tgttgcctaa tccgctatta ttttctaata ttggaatttt ttaaatcggt ataataaacc
     5341 cttgtcgacg tttatccgta cgtcattgta aattttgatt aagccaatga cgagatttga
     5401 actcgcgacc tgctgattac aaatcagctg ctctagccag gctgagctac attggcatga
     5461 tgtatcagaa taattaagat aatttaaaat gttcttaaag caccatatat ggtatatcac
     5521 tttaataatc accagatctg aaaaatctct atgcagttga agtatatccc accaaaaaaa
     5581 ctaaaggtac tcatcatcat gttttttgta gcagcagctt ttggaatttt tgtaggatta
     5641 gtaatagcaa aaggtggtca aggattctat attacactgc ttggagttgt taatttatgc
     5701 ttaggtggat ttatggcgta tcttttaatg actcaaaaac ccaaagtacg tgatagtagg
     5761 aaaagaaaat aaaacataat ctagttaagt tcttaatata gctgaaaatt acaaataatt
     5821 aatgcttgat ctagttgcca aaaaattatt tcttacaaaa ggtgttggta ttgctgatga
     5881 taagttaaca agttttgaat ttgcattgag aaacgctgga atttctggaa caaacatagt
     5941 attgatctct agtatttttc caccacgtgc aaaattaata cctcgaaaag aaggtctgaa
     6001 actaatcaaa cctggccaaa tactatttac aatttattca agaacacaaa caaacgaacc
     6061 tcacagactg atttctgcat ctgtaggagt agcacagcca aaagatagat caaaatatgg
     6121 ttacctttct gaatacgatg cctatggaca aaatgaatca aaggcgggag attatgcaga
     6181 ggatatagct gcacaaatgc tggcatcatc acttggaatt aaatttgatc tcgataaaag
     6241 ttgggacgaa aagcgccaac aatggaccat ctctggccaa atctataaga caaaaaatat
     6301 cacacaatct gcaaagggtg ataaaaatgg aaaatggaca actgtgtttg ccgcagcagt
     6361 tttaatagtt tagatttttt taattgtttt aatcttgatt ataactagtg ctggaacaat
     6421 aaaatacatt ccaatattta acaggatgac accaattcca tatccaagca tctcagattc
     6481 tgagtcgatg tcagcatatt gtaataatgt aagtgaagtt agaagtggag ttagtgttag
     6541 ttttacaacc tccttgaata ctgggttttc tcgttcataa tcagctattg ttggagaaaa
     6601 tgaataatag aactgattaa aactagtcat gaatgcagta ccagattcag ttttaagtac
     6661 agtgttgtca cgaatctctc gtaagaattg gacttgtggt gccatctcag aaccaaacgc
     6721 agctgttgct atgagacatc caccacgctc tttgaactct gttgcctgtt ttactgcttc
     6781 atcataactt ggattatctg aagcattttc ttcaactatt acatcattat gaattacagg
     6841 ttctgttcca tcattattac caatgatcaa atacatatct tctctatcta ctgatgactc
     6901 tgtaccgaac aaatacttac cactaattac ttcacctaat tcagaggttg aagaagaatc
     6961 aaaataccat ccaccagttt tgagttcctc tatatttgct gggtcaataa tcaaattaga
     7021 attcaattct gttatctgtg agttttgatc tggaataact gtcaaagata ttattgagtt
     7081 tagaggataa aatccatctg taaatacctt agagcgttct aatgtatcaa ccatcagcag
     7141 ttccaacagg cttattctca tattggttat atcgtattgt ccccaatctt ccatcagaac
     7201 tgttattgag cgtaaaactc tgttatctac tggaactagg ctgaatgaaa atacagaatc
     7261 tttatgttca gccatttttt gggcatgttt aaaaaaacca ccaccattat ggatctcatc
     7321 tttaacaaac ggcgcagtta tttcatcaaa atgttctact gatggttttt ttgggattat
     7381 gtaagtcact gttagttttg atttgtataa atctgaagtc aagcccattc tattgatgaa
     7441 tacagagtga aattctgcat caataccaaa ggatttattg ataacaccta tgaatgaatc
     7501 acccactgct tgcgcatcag tctttaattc aactaatcct ctgatttcag aaggccttgt
     7561 tacatctatc atgacacaag aaaattcatg taattcataa tcaacaactt cttctacacc
     7621 tggaacacaa tcatactgat ttgtgtagct aatcccgatt aatctaggag aattaagtat
     7681 cattgattcc aatagttctg ggatcattat atcatcagga tttttactca tcacacctaa
     7741 tgatactgat gttttaccac ccttttcatc tattactaat tgtgcagttt ctgaccatga
     7801 tgaatattct atcgattctg aaaatgattg tggtatgtaa aaaacaaata gcagtgcaac
     7861 tgtggctaat aataaaatct tatacatagt tatgtgccta tttttttcta ttaattactt
     7921 tactatcaaa cctttaaatt aaatatattt tttcaatatc ttgacatcga ttcaaaccgt
     7981 tggggaacaa gctggcagtt agatggctct aactgccagc cccatattta ttaaattttt
     8041 aaaagataaa atcatccaag tctagacaat tcatcgactt gggttttgat ataatcaaaa
     8101 ccatcttgcc agaatctaga ttttgtgata tccatgccaa actcctgaag caatttttca
     8161 ggtttctttg aaccacctgc agataggatt tctgtgtatg tagaagcaaa tcctgaacct
     8221 tctcttttgt atttctgaaa taaagaaagt gacagcaagt ttccaaaagc atatgaatag
     8281 cagtaaaatg gagcatggta aaaatgtggt atacaactcc attctgtttg aaaatcttcc
     8341 gaaacattaa cggaacttcc aaactgctcg ttaagattat gaagataagt ttttgagatc
     8401 tcatctaccg ttgtactatt tgcaatttgt tgatgtgcag aaatctcgaa taatgtaaaa
     8461 aaagactgtc taccaattgt tgcataaaaa tcatctattt tttctgacag cattattttt
     8521 ctttcctcgt cacttactaa ttcagataaa ttatcataca gcaacatttc agaaaatgta
     8581 gatgctgttt cagctaatgg tagtgatgca tctgatatta gtatagaatt ctttgaagca
     8641 catatgctgt gtatggcatg accgatttca tgtgataggg taaacacatc acgtgatcta
     8701 cctgtaaaat tcatcaatac atatggtatt ttgtttggag atggtgtgct acaaaatgct
     8761 cccgatacct ttccaggtct taattctgaa tcaacatgat tttgtgaaaa tacactactt
     8821 gcatattttg acagtgtggg actaaattta tccagtgatt tcagtaccag ttttaccgat
     8881 ttatcatacg agtatgtttt ttcttttgga tttgattttc taggagcata tagatcatac
     8941 cgtctaatct ttttcatgcc cagtaatttt gctttttctt tgaaatattt ttgaaaaact
     9001 ttggcattcc ttctacatac gtcgagtaat gtttttactg ttttttcatc gacattgttg
     9061 gcaatatttc taatcatgat tggagatgaa aaacctctca taccaattcc ttcatccttt
     9121 aggtttagaa caatattttg ataaatttct ccagttactc ccttgttctc agaatatttt
     9181 gaaaataatg atttgtatgc tgcttctcta acttttgatt ttttatttct aacaagtgta
     9241 gttagttctt ccctattcat cctctttttt ttcccatcaa ttttgacaac atacacaaat
     9301 gcattggtta tcttgtcgta taattttacc agtgcagaag ctccagttac atcaagtgta
     9361 ttgatgattt tttcttcagg ttcactaagt gaatattttg ctaaaattct tttgaattca
     9421 agatactgtg aatattcacc tgatgatttg atgagacggc gtgcattttt ttcatcaatc
     9481 tgttttttcc accataaatc aaaaaataat aagcgatttt ctatttcaga accaaactga
     9541 gagatccgtg ttagaagtga agttgcctca tcagactgtg tattttcaga atattgtaat
     9601 gatgcatatc cacctatgat actagatttt tccgtaatat gctcaatttt gtgtatcaat
     9661 tctaaaagtt tcttagagga tattttttta tttagaaatt tcttctgttt ttcaaattcc
     9721 ctagcataat tattcaattc ggctaatttt ttatcaaatg tagatctggt tgggtttttt
     9781 aacagatcgt caagactcca ttttgaaagt ttgtattctg tcatattgat caataatagt
     9841 ccttgtgaat ataacaccag cgccatgatt tgtcaggcac tgcaaccatt acaggatgac
     9901 ctgtttcttt gaaatgcttt gtcgcgtgta aaccaactga agagtcgcag catccaacat
     9961 gtccacatgt aagacacatt cgtattgcga cccatggaag tttttccttt tcacattctt
    10021 cacatccttt ggtatttggt gatacttcat tagacgcttc accaaaatgc tcgcactcat
    10081 ctgtcatatg acattttcat ggtatattcg tatatatttt cacaactttt ccaatgccta
    10141 aataacacat caatcaagaa aaaccggatc cgtcgtctag cttggtttgg ataccgcact
    10201 cacactgcgg gggtcaaggg ttcaaatccc ttcggatcca ttttaactac tgcttgattt
    10261 cataatataa tatggataaa agggactgga ttatttacaa gaaaaaagag attggttggg
    10321 ctattggaat aggcattatg actgtcggat ttttgttcct tgtaaacaac tttggattag
    10381 gaatgttcag accatagaat cacaaatcaa acatgttttt tacattacat aagttggttt
    10441 ttttcagatc tatgctaatt tgcaaacatt tttctgcctg taattcagat gacaaataat
    10501 tggtttgcac gccaacacct agggagatag cattgttatc tctctagggc ataatgatta
    10561 gctttttgaa taaccgtcta gttttagttt tgttggttgg ctagattagg aaatatactg
    10621 aatacctgac gttttacggc agataattat acggtttatt atttgcataa aattgctgga
    10681 tgccaattaa cactaatcaa atcccttcgg atccattttt acatatgtta gggcataaaa
    10741 caacatgaaa gtaacaaaaa tttctgcggt cacattacca gtttctgaca tgaaaaagtc
    10801 agtcgatttt tactccaaaa ttccaaactt taagatagtt tatggcggac ctgatagcca
    10861 attcacatca tttctgatag aggatacaac aaagtcatat cttaatctga aattgaatga
    10921 agtacatcca ccaaattttg gtagaattat tttttatgtt gataatgttg atgatctttt
    10981 tgcatacatg gaaagtgacg aaacaatttc ggggctaggt aaactagaat ccaagccgga
    11041 ggatgcagaa tggggtgagc g
//
LOCUS       DASCPJ010000036         1574 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000675699, whole genome shotgun sequence.
ACCESSION   DASCPJ010000036 DASCPJ010000000
VERSION     DASCPJ010000036.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000675699"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            109..822
                     /locus_tag="QGF01_01910"
     CDS             109..822
                     /locus_tag="QGF01_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HJN19686.1"
                     /translation="MIVDISELSVNYDGIDALQKISFGINEGDFLGIIGPNGAGKSTL
                     FNCMLRIHLQYQGVIKFFNKDIRKSEEYLTHIGYVPQKPVVDRNFPATIREVVSMGQI
                     NNDPDKVDEALQKVWMHELADRRIGDLSGGQQQRVFIAKALVNSPKILVLDEPVTGVD
                     IHNKDLFFQILSELNSKGKISIIWSSHDLDAVERLANKVACLNKTLFFHGISQEFFND
                     EEMMKKYSETSMQMHMHHH"
     gene            829..>1574
                     /locus_tag="QGF01_01915"
     CDS             829..>1574
                     /locus_tag="QGF01_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HJN19687.1"
                     /translation="MIFDILSFGFMQNAIVSGIAISLICSTVGLFLVLRKFSLFGDAL
                     AHSAFGGVALGLFLGFYPLWAAYAVSILSALGLTKIRQKYDISGDAIIAILLSSGIAV
                     GIVLISLSGGFSIDIFSFLFGSVLLVSTENVIMILGLSAAILIILIICYKKFMYITFS
                     EEQAQVSGIPVQKLNYLLIAIAGVTVVTSMQLVGVLLVSALFVIPNVTAMMFKRSFKQ
                     TIILSMSFSVFSTVAGIMISYPLDIAPSGMV"
BASE COUNT          535 a          217 c          295 g          527 t
ORIGIN      
        1 cgctgatgtt ttaattttat cacctgcagc cacagaacat atagaaaata agaattattt
       61 tgagagaatg caagaaaata cttctaatct aaaggaggca ttatgtaatt gatcgtcgac
      121 atttcagaat tatcagtaaa ttatgatgga atagatgcat tacaaaaaat aagttttgga
      181 attaatgaag gagattttct tggaatcata ggtccaaatg gtgcaggaaa atccacatta
      241 tttaattgca tgttgaggat tcatttacaa tatcaaggag taatcaaatt tttcaacaaa
      301 gacattagaa aatcagaaga atatctaaca catatcggat atgtcccaca aaaaccagtc
      361 gttgatagaa attttccagc aacaataaga gaagtggtga gtatgggcca gataaataat
      421 gatccagata aggtagatga agcattacag aaagtatgga tgcatgaatt agcagatagg
      481 cgaataggag atttatcagg cggtcaacaa caaagggtgt ttattgcaaa agcattagtc
      541 aattctccta aaatattagt tctagatgaa cctgtcacgg gagtagatat tcacaataag
      601 gatttatttt ttcagatttt aagtgaacta aactcgaagg ggaaaatatc catcatttgg
      661 tcatcacatg atcttgatgc agtagaaaga ttagcaaata aagttgcatg tctaaataaa
      721 acactatttt tccatggtat ttcacaggaa ttctttaatg atgaagaaat gatgaagaaa
      781 tactcagaga catctatgca aatgcatatg caccatcatt gatgagatat gatttttgac
      841 atcttatctt ttgggtttat gcaaaatgct atagtttcag gcattgcaat ttcactaatt
      901 tgttccacag taggattatt tttagttctt agaaaatttt cgttgtttgg tgatgcatta
      961 gcacattctg catttggtgg agttgcattg ggtttatttc tcggatttta cccattatgg
     1021 gcggcatatg cagtttcaat tcttagtgca ttaggattaa caaagattcg acagaaatat
     1081 gatatttcag gagatgcaat tatagcaatt ttactttcat caggaatagc agtaggaatt
     1141 gttctgatta gtttatcagg aggattttca attgatattt tctcattttt gtttggaagt
     1201 gttttgcttg tgagtacaga aaacgtaatt atgattttag gattgtctgc agcgatttta
     1261 ataattctta tcatatgtta caaaaaattt atgtacatta cattttctga agaacaagca
     1321 caagtttcgg gaatcccagt tcaaaaattg aattatttgt taattgcgat agcaggagtt
     1381 acggtagtaa catcaatgca attagtaggc gtacttttgg tttcagcact ttttgtaatt
     1441 ccaaatgtca ccgcaatgat gtttaaacgt tcatttaaac agacaattat tctttcaatg
     1501 tcattttcag tattttccac agtggcagga ataatgattt catatccgct agatattgca
     1561 ccatcaggaa tggt
//
LOCUS       DASCPJ010000037         5876 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000679292, whole genome shotgun sequence.
ACCESSION   DASCPJ010000037 DASCPJ010000000
VERSION     DASCPJ010000037.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000679292"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="QGF01_01920"
     CDS             complement(<1..1151)
                     /locus_tag="QGF01_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HJN19688.1"
                     /translation="MQIGLLGKANVGKSTFFSAATETPVQIGNFPFTTIKPNIGVAYV
                     NTTCACKHFRIEHNNPLCISGIRFVPIKLIDVAGLVPGAHEGKGLGNQFLDDARQAEA
                     LIHVVDISGSTDIQGQQISVGTHDPNEDIKFVEEEFDLWFKQILEREWQKLSSEIDKK
                     STKPVDAITERFSGLGVKTHHVHHVLQNLNLLNKKPKEWDSSDLVKFAQMLRKNTKPM
                     LIAANKADLCEDLGISDEIHKNRDIVNCSAETELVLKKAAKANMIDYIPGNEKFVLNK
                     NMNMSSAQKEALKLVENVLSKLNSTGIQTALNYTVFRTLKMIVVFPVEDETKLSNKDG
                     EVLPDAKLLPDNATAKNLAEVIHQDIAKGFLFAVDVKTKQRIGADHQLK"
     gene            1223..1780
                     /locus_tag="QGF01_01925"
     CDS             1223..1780
                     /locus_tag="QGF01_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024635.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN19689.1"
                     /translation="MLVLTGNPGVGKHTTASEIMKQNAMYEIIDINNLAIELGLTEKA
                     KETLEVYVAELKIKMKQKVSKNSLIVGHLAPYILDESDVDVAIVLRKNPLDLIKVFKD
                     RGYSKEKIRSNTAAEFIGVTFNDSITSFGEEKTFQIDTTNKTPEQVTSIIDKIVHGKN
                     KGDIVDWFHLVDKNAAIQELLDIIE"
     gene            1814..3328
                     /gene="tgtA"
                     /locus_tag="QGF01_01930"
     CDS             1814..3328
                     /gene="tgtA"
                     /locus_tag="QGF01_01930"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HJN19690.1"
                     /translation="MFEIKYSDLAGRIGILHSNHGKIETPAYVPVIHPVKQTIPTKKI
                     REMGFDLVITNAYITMKRYGEEAIKRGIHDVIGFDGAIMTDSGGYQVLEYGDVDIAPP
                     DMADFERKIMTDFAIPLDKPTGYGLSKKIAKSYVNQTLKDSKKTLESRKDNGQIWLGP
                     IQGGEHQELVKSSTKHLIDYGFPMLALGSPVEFMESYEYKLLASMIITAKKQIPNNIP
                     LHLFGAGHPLTIPLAVALGCDTFDSASYILYAKHDRYIEEDKTTHLADIKHFSCLCEV
                     CTKFKPKELLALKSEDKINKIALHNLFAIKSEVDRVKESIMQGRLWEYVMMKMRAHPK
                     LFEAIDVFIKNSDFFLSTTPKFKKKAIFVFSKEDQYRPEILAYQNIVKKFKTRKKSIV
                     ITKNTSTKPAYLSNEYMSMKKKFKDDESVQFCYYNPFIGIIPLELSDIYPASHYEMPR
                     SDFMPEDFPMFTQIWNTFFSRNNFDVLYISKKDPFIKYFVKMLPKNIKRKFLKK"
     gene            complement(3358..3660)
                     /locus_tag="QGF01_01935"
     CDS             complement(3358..3660)
                     /locus_tag="QGF01_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJN19691.1"
                     /translation="MPIAILPDVDEQRCIGCALCVEICTSLGPDVLRVKPVDGWKRGK
                     AFVFYPERCISDGACIGVCPTKSIFWMRPMNYTAGQPVPLHKNGVFVKGWAEDAAL"
     gene            complement(3790..4002)
                     /locus_tag="QGF01_01940"
     CDS             complement(3790..4002)
                     /locus_tag="QGF01_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19692.1"
                     /translation="MGRSYDEWIKQQDQALVAKVRAGDESQKPLLNQINWIWVANLMN
                     QKADLNPSSAELLDWVTSGQIDAMRK"
     gene            4112..5380
                     /locus_tag="QGF01_01945"
     CDS             4112..5380
                     /locus_tag="QGF01_01945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJN19693.1"
                     /translation="MKIKVLGAANEVGRSGFLVNSDNTNLLLDYGVMFARKRTDPPSY
                     PLHVKPKEIDAAVITHAHLDHSGCIPSLFNGGNTNVYSTPPTIDLSVLLIEDMLKIEK
                     NKHDFDYRHVNNMIRNSKEIGYRQPVNVGDIKFELRSSGHVIGGSTVIVEAKGKRLFY
                     TGDINLRGSRMLPPADLDIGDIDLLITESTYSQSQQTPRAESEKGLLEFASEVMDRKG
                     VLFIPSFSVERSQEIACVLKNANFKYPVIMDGMALKVNDIMFRYPEYLKNVNIFSDAV
                     NGATLIRDHSDRKRAISEPCVVISPAGMLVGGNAVYYMQNLASSDKNGVAMVSYQGEG
                     TPGKKLLETGKISLKGKDMNVKATVKRFEFSGHADRDSLFEMLKNIKGNPKILTVHGD
                     RESCTKFSEEIQQKFGFDSHAPKLGEEITI"
     gene            5361..>5876
                     /locus_tag="QGF01_01950"
     CDS             5361..>5876
                     /locus_tag="QGF01_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HJN19694.1"
                     /translation="MKKLPYNIHDTINSGQVFLWQNQENVWFGIDGQNTIQISRKPSQ
                     TVILSKKARNFLRDDDDFSKIVKCISKDKIVKKAIKHSPGLRITRQDPFQCYISFIAS
                     ANSNIAKIRMCLEKLCRRFGTKARFKNRDFFLFPKPHVLASATLEEVQQCGLGYRSSY
                     IIDASRAVLSEK"
BASE COUNT         2011 a          949 c         1036 g         1880 t
ORIGIN      
        1 tgttttaatt ggtgatcagc accaattctc tgttttgttt taacatctac agcaaacaaa
       61 aatccttttg caatgtcttg gtgtattact tctgcaagat tttttgcagt agcattatca
      121 ggtaacagtt tggcatcagg aagaacttct ccatctttgt tagataattt cgtttcgtcc
      181 tctacgggaa aaactacgat cattttcagt gttctaaata ctgtataatt cagggcagtt
      241 tgtattccag tggaatttaa ctttgacaaa acattttcca caagtttcaa tgcttctttt
      301 tgtgctgaag acatattcat atttttgttt agaacaaact tttcatttcc tggtatgtaa
      361 tcaatcatgt ttgctttagc agcttttttt aagactaatt cggtttctgc actacagttt
      421 acaatatctc tatttttgtg gatttcatct gatattccca aatcttcaca caaatcagcc
      481 ttgttcgcag caattaacat gggtttagta ttttttctta acatctgcgc aaacttgaca
      541 agatcagaac tatcccattc ttttggcttt ttgtttaaca ggttcaaatt ttgtaaaaca
      601 tgatgaacgt gatgagtttt aacaccaagt ccactaaaac gctctgtgat agcatcaact
      661 ggtttggttg attttttatc tatctcactt gaaagtttct gccattcacg ttcaagtatt
      721 tgtttaaacc ataaatcgaa ctcctcttca acaaatttaa tatcttcatt gggatcatgt
      781 gttcccacgc ttatctgctg tccctgtata tcagtagaac cagaaatatc tacaacatga
      841 attagtgctt ctgcttgtct agcatcatct agaaattgat ttcctagtcc cttgccttca
      901 tgtgctcctg gtacaagacc tgcaacatca ataagtttga ttggtacaaa tcgaattcca
      961 gaaatacata atggattatt atgctcgatt ctaaaatgtt tgcaagcaca agtagtgttc
     1021 acatatgcaa cgccaatatt tggcttaatt gttgtgaagg gaaaatttcc aatctgaacc
     1081 ggagtttctg ttgcagcaga gaaaaatgtt gatttcccaa cgttagcttt tccaagcaga
     1141 ccaatttgca tgacatagaa tgatggtttt atacatatta gtattacaga gatttttaat
     1201 tcggttagtt ttgaatatgt gtatgttagt tttaacgggg aatccaggtg tgggtaaaca
     1261 tacaactgca tctgaaataa tgaaacaaaa tgcaatgtat gaaattatag atattaacaa
     1321 tctagcaata gaacttggat taaccgaaaa agcaaaagaa actttggagg tttatgttgc
     1381 agaattaaaa ataaagatga aacagaaggt ttccaaaaat tccttaattg ttggacattt
     1441 ggcaccatat attctggatg aatctgacgt tgatgtggct atagtattaa ggaaaaatcc
     1501 attggatttg attaaagtgt ttaaagatag aggatactct aaagaaaaaa tcagatcaaa
     1561 tactgcggct gagtttatag gagttacatt taatgattct attacatctt ttggcgaaga
     1621 aaaaacattt caaattgata caacaaataa aactcctgaa caagttacta gtattattga
     1681 taaaatagtt cacggaaaga ataaagggga tatagtagat tggtttcatc ttgttgataa
     1741 aaatgcagca attcaggagt tgctagatat catagaataa taatttaata atgtacaaaa
     1801 ggtatgctaa caaatgtttg aaataaaata tagtgatttg gcaggtagaa tcggtatttt
     1861 acattccaat catggaaaaa ttgaaactcc tgcttatgtt ccagttattc atcctgtaaa
     1921 gcaaacaata ccaactaaaa aaattcgtga gatgggtttt gatcttgtga tcactaatgc
     1981 atacataaca atgaaaagat atggcgaaga agcaatcaaa agaggaattc atgatgtgat
     2041 aggttttgat ggcgctataa tgacggattc tggtggatat caagtattag aatatggtga
     2101 tgttgacatt gcacctcctg atatggctga ctttgaaagg aagataatga cagactttgc
     2161 tatacctctt gacaaaccaa caggttacgg tctaagtaaa aagatagcaa aatcatatgt
     2221 taatcaaact ctgaaggatt ctaaaaaaac actagagagt agaaaagata atggacaaat
     2281 ttggttgggt ccaatacaag gaggtgaaca tcaagaactg gtaaagagtt caacaaaaca
     2341 tctaattgac tatggatttc ctatgttagc ccttggcagc cctgtggaat tcatggaatc
     2401 atatgagtat aaacttctag cgtcaatgat aataacagct aaaaaacaaa ttcctaataa
     2461 tataccatta catctttttg gagctggtca tcctttgaca attcctttgg ctgtagcgct
     2521 tggatgtgat acttttgatt ctgcatcata catactatac gccaagcatg accgatacat
     2581 agaagaagat aaaacgactc atttggctga cataaagcat ttttcatgcc tatgtgaagt
     2641 atgcacaaaa tttaagccga aagaacttct ggcattgaaa tctgaagata aaataaacaa
     2701 aattgcactt cataacctat tcgccattaa atctgaagtg gatagagtca aagaatcaat
     2761 catgcaggga aggctttggg agtatgttat gatgaagatg cgtgctcatc caaaactatt
     2821 tgaagcaatt gatgttttca taaagaattc tgattttttt ctcagcacta ctccaaaatt
     2881 taagaaaaaa gcaatctttg tgttttcaaa agaagatcaa tataggccag aaattcttgc
     2941 ttatcagaat attgtcaaaa aattcaaaac aaggaaaaaa agtatagtta taacaaaaaa
     3001 tactagtact aaacctgcat atctttcaaa tgaatatatg agtatgaaga aaaaattcaa
     3061 agatgatgaa tcagttcaat tttgttatta taatccattt attggtataa taccattaga
     3121 attatcagat atctatccag catcacatta tgaaatgcca agaagtgatt ttatgccaga
     3181 agattttcca atgtttactc aaatatggaa tactttcttc tccagaaata actttgatgt
     3241 tttatacata tcaaaaaaag atccattcat aaaatatttt gtgaaaatgt taccaaaaaa
     3301 tatcaaaagg aaattcttaa aaaaataata aaaaaaagtt tagtgggaag atacagatta
     3361 tagtgctgcg tcttctgccc aaccttttac gaatacaccg tttttgtgaa gaggtacagg
     3421 ttgtcctgca gtatagttca ttggtctcat ccaaaagatt gactttgtag ggcacactcc
     3481 aatgcatgct ccatcagaga tacatctctc aggataaaat acaaatgctt ttcctctctt
     3541 ccaaccatca acaggtttta cacggagtac gtcaggtcca agagatgtac agatttctac
     3601 acaaagtgca catccgatac atctctgttc atctacgtct ggaagtattg ctattggcat
     3661 ttatttcact gatcatccag tcacactttg ctatttaaac catgattgaa atctataacc
     3721 ctgttataga atagatcgtt aggtgaaact aataaaaaaa acaaaaaaaa tacgtgatgg
     3781 ttacacgctt tattttctca ttgcatcaat ttgaccagag gttacccagt caagtaattc
     3841 tgcagatgaa gggtttagat ctgctttttg attcatcaaa ttagcaaccc aaatccagtt
     3901 tatctgatta agaagtggtt tttgtgactc gtcaccagct ctgaccttag ctacaagagc
     3961 ctgatcttgc tgttttatcc actcatcata acttcttccc atgagagatc gatcttaggt
     4021 ttgcctaatt aaagcttatg gtaattttcc tatttgtaat ttgatcggtt acactaagaa
     4081 gtttttaatg tagtggctta agatattttc cgtgaagatt aaggttttag gagctgcaaa
     4141 tgaagttggt agatctggat ttttagtaaa ttctgacaat acaaatcttc ttttagatta
     4201 tggtgtcatg tttgccagaa agcgtactga ccccccttca tatccattac atgtaaaacc
     4261 aaaagaaatt gacgctgcag ttatcactca cgcacatcta gatcattcag gatgtattcc
     4321 ctctttgttt aacggaggaa atactaatgt ctactctacc ccacctacta ttgatctgag
     4381 tgtattactt attgaagata tgctcaaaat tgaaaaaaac aagcatgact ttgattatcg
     4441 ccatgtcaat aatatgattc gaaattctaa ggagattggt tacagacaac cagtaaatgt
     4501 tggtgatata aaatttgagt taagatcttc tggacatgtt ataggtggta gtacagttat
     4561 tgttgaggca aaaggtaaac gactgtttta tacaggagat atcaatctga ggggctcccg
     4621 tatgttgcct ccagctgatc ttgatatagg agatatcgat cttttgatta ctgaaagcac
     4681 ttattcgcaa tcacaacaaa cgccacgagc tgaatctgag aaaggcttac tcgaatttgc
     4741 tagcgaggtt atggatagaa aaggagtttt attcattccc tcattttctg ttgaaagatc
     4801 acaagaaatt gcatgtgtgt taaaaaatgc aaacttcaag tatccagtaa ttatggatgg
     4861 aatggcatta aaagtaaatg atattatgtt tcgttatcct gaatatttga aaaatgtgaa
     4921 tatatttagt gatgcagtta atggtgctac tctgatcaga gatcatagtg atagaaaaag
     4981 agctatttca gaaccatgcg ttgtcatttc tcctgctggt atgctggtag gtggtaatgc
     5041 tgtatactat atgcaaaatc ttgcttcaag tgataagaat ggagttgcta tggtttcata
     5101 ccaaggagaa ggtacacccg gaaaaaaatt attagaaaca ggaaagatat cattgaaagg
     5161 aaaagatatg aatgtaaaag caactgtaaa acggttcgag ttttctggtc atgccgatag
     5221 agattcattg tttgaaatgc tcaaaaatat taaaggtaat ccaaagattt taacggttca
     5281 tggtgaccgt gaatcttgca caaaattctc agaagaaatt cagcaaaaat ttggttttga
     5341 ttctcatgca cctaaactag gtgaagaaat taccatataa catccatgat accattaaca
     5401 gtggccaagt tttcttatgg caaaatcagg aaaatgtatg gtttggaatt gatggtcaaa
     5461 atacaattca gattagccgc aaaccctctc aaactgtaat attatcaaaa aaagctagaa
     5521 attttttgag ggatgatgat gatttcagca aaattgtgaa atgtatatca aaagataaaa
     5581 ttgtgaaaaa agcaataaaa cactctcctg ggctgcgtat tacacgacaa gacccatttc
     5641 aatgctacat ttcatttatt gcctcagcga attcaaatat agcaaaaatt agaatgtgtt
     5701 tagaaaaatt atgtagaaga tttggtacta aagcacgatt caaaaatagg gatttttttt
     5761 tatttcctaa acctcacgtt ctggcaagtg ctacattaga agaagtacag cagtgtggat
     5821 taggatacag atcatcatac ataatagatg catctcgcgc tgttctatca gaaaaa
//
LOCUS       DASCPJ010000038         3664 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000682792, whole genome shotgun sequence.
ACCESSION   DASCPJ010000038 DASCPJ010000000
VERSION     DASCPJ010000038.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000682792"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="QGF01_01955"
     CDS             <1..485
                     /locus_tag="QGF01_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19695.1"
                     /translation="QFFKVWIKAKFSLDFEIKCDEMITKPRIILQRLDTHSLLKDHTE
                     RGKDIFHFYLCHFRPLWTDCLCEGYHAENFCMLRWEKPKNENDVIAMAEKNCTAVSHE
                     ITHELLRQSGYKRYIEDVHEVWQKHLFGDLPFEQYGEDFKLTSKKPSFLALDTTLFGL
                     "
     gene            complement(510..1142)
                     /locus_tag="QGF01_01960"
     CDS             complement(510..1142)
                     /locus_tag="QGF01_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19696.1"
                     /translation="MVKKGFPSFGVSQSGAFVAALQNYNLPDFILALVAKDCDSDLLV
                     RGRINDRLASMNDQSLETLHRVFVDCEEDLGGKFAQYRFFSYVSSMYHKCEVLINETI
                     PGKSNKNHKIPIAIKNNGMYVAVGFNKPKGNSVSEKDVIKFHDIVDDVKNGDHGTQLI
                     DAIYGSSVGFKMKAIIALKQLSKSRDENPETKVNFLTANFENRIYSVTKC"
     gene            1277..3568
                     /locus_tag="QGF01_01965"
     CDS             1277..3568
                     /locus_tag="QGF01_01965"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HJN19697.1"
                     /translation="MLLGKIEIDTTVEMVQNVYSKLEKNVLVFKKTLNRDLTLSEKIF
                     AGHLEEKFLSKILAEGTEYVFLRPDRVALQDVTGQMVMLQFMQAELKSVVLPTTIHCD
                     HLIQARTEGKSDTKLAIFENNEVYKFLESSASKFGAGFWKPGAGIIHQVVLENYAFPG
                     GLMIGTDSHTPNAGGLGMLAIGVGGVDAAETMAGVPWELLYPKRVGVYLKGEMNGWTS
                     AKDVILYVAGELSVSGGTNSIIEYFGPGTKSISCTGKATITNMGAEIGATCSIFPYDE
                     RMEVYLKSTGRKQIADLANKYKEHLTADSDVEQNPEKYFDKIIEIDLAKLEPHIVGPH
                     TPDLARPISQLADDVKNKNYPDNISVALIGSCTNSSYEDMSRSASLAKQAQSKGIKSK
                     IPLLVTPGSEQIRATIERDGQIDSLKSIGATVLANACGPCIGQWHRPELKQGERNTIV
                     TSYNRNFPGRNDGKRETMNFIASPELVTALALGGNLSFNPLNDSLVASDGTKFRLDPP
                     ERAPDVPDREFVNVEDIYLSPSENPDTVQVLIDPESERLQKLEPFTAWDGQDLNNLHI
                     IMKAKGKCTTDHISPAGPWLRLRGHLDKLSDNLLLGAVNAFNDEVGKAKNLLTNKIEN
                     CAEIARQYKKQNISWIIIGDDNYGEGSSREHAAMTPRYLGCKVVIAKSFARIHETNLK
                     KQGVMALTFDNPKDYEKIHEDDKISINSLSDISPGKSVDCTVEHVDKTSEKIILNHSY
                     NDLQIEWFRAGSAMNFLRKNQKD"
BASE COUNT         1289 a          592 c          676 g         1107 t
ORIGIN      
        1 ctcaattttt taaggtgtgg ataaaagcaa aattttctct tgattttgag attaaatgtg
       61 atgaaatgat taccaaacca agaataatac tacaaagatt agacactcat tcattactaa
      121 aggatcatac agagcgtgga aaggatattt tccattttta cttgtgtcat tttagaccat
      181 tatggactga ttgtctttgt gaaggctatc atgcggaaaa cttttgcatg ttgagatggg
      241 aaaaaccaaa aaatgaaaat gacgtgatag cgatggcaga aaagaattgt accgctgtat
      301 cacacgaaat aacgcatgaa ttattgcgac aatctggata caaaagatac atagaagatg
      361 ttcacgaagt ttggcaaaaa catctttttg gtgatctacc atttgaacaa tatggtgaag
      421 attttaaact aacttcaaaa aaaccatcat tccttgcatt agatactaca ctttttggat
      481 tatgattcaa tccaattgat taatctactt taacattttg taactgagta aattctattt
      541 tcaaaatttg cagtcagaaa gtttacttta gtttcaggat tttcatctct agatttactt
      601 aattgtttta atgcaataat agctttcatc ttgaatccta ctgatgaacc atagattgca
      661 tcaattaatt gtgtaccatg atcaccattt tttacatcat caacaatatc atggaattta
      721 ataacatctt tttctgagac agaattgccc tttggtttgt taaatccaac agcgacatac
      781 ataccgttat tttttatagc gattggaatt ttgtggtttt tattagattt tccaggtata
      841 gtttcattaa tcaaaacttc acatttgtga tacatgcttg atacataaga aaaaaatcta
      901 tactgagcaa atttaccacc tagatcttct tcacaatcaa caaaaactct gtgtaatgtt
      961 tctagtgatt ggtcattcat actagccaac ctgtcattga ttcttccacg aacaagtaga
     1021 tctgaatcac aatcttttgc aactaatgca agtataaagt caggtagatt ataattttgt
     1081 aaagcagcca caaacgcacc agattgtgat acaccaaaac taggaaagcc tttttttacc
     1141 aattaaatcc cccgcaaaaa atattatgcg aaattaatat gaggaacaat agtctagaat
     1201 atcgtttttc tcttataatt gtatgtaaaa atttactgaa caagttaatc cgtttataaa
     1261 cgaaaaatcc tacaagatgt tattgggaaa aatagaaatt gacacaacag tagaaatggt
     1321 acaaaatgtt tattctaaac tagagaagaa tgttctagta ttcaagaaaa cactcaaccg
     1381 tgatctaact ttatctgaaa aaatttttgc tggacatcta gaagaaaagt ttctgagcaa
     1441 aattttggca gagggaacag aatatgtttt cttacgtcca gacagagttg cacttcaaga
     1501 cgttactggt caaatggtta tgcttcaatt tatgcaagca gaactaaaat cggttgtact
     1561 tcctacaaca attcattgtg atcatctaat acaagctaga accgaaggaa aatcagatac
     1621 aaagctagca atatttgaaa acaacgaagt ttacaaattt ttagaatcat ctgcaagtaa
     1681 attcggtgca ggcttttgga aaccaggtgc aggtattatt caccaagtag ttttagaaaa
     1741 ctatgcattt ccaggaggtt tgatgattgg tacagattct catacaccaa atgctggggg
     1801 acttggaatg ttggcaattg gagttggcgg tgtagatgca gcagaaacaa tggctggtgt
     1861 accttgggaa ttactatatc caaaacgtgt tggtgtttat ctgaaaggtg agatgaatgg
     1921 ttggacttca gcaaaggatg ttatactata tgttgcagga gagctttcag tatcaggagg
     1981 aacaaattct ataatagagt attttggccc tggtacgaaa tctattagct gtacgggaaa
     2041 agctacaatt actaatatgg gtgcagagat aggtgctacc tgctcaattt ttccatatga
     2101 tgaaagaatg gaagtttatt tgaaatcaac tggaagaaaa caaattgctg atcttgctaa
     2161 caaatacaaa gaacatctaa ctgcagattc ggacgtagaa cagaatcctg aaaaatattt
     2221 tgataaaatt atagaaattg atctggcaaa acttgaacct cacatagttg gtccacacac
     2281 accagatctt gcaagaccta tttcacaatt ggctgatgat gtaaaaaata aaaattatcc
     2341 tgacaacatc tccgttgcat tgattggaag ttgtacaaat tcttcatatg aggatatgtc
     2401 caggtctgca agtttagcaa aacaggcaca gtcaaaagga attaaatcaa aaattccatt
     2461 actagtaact cctggctcag aacagattag ggcaactata gaaagagacg gtcagattga
     2521 ttcactaaaa tcaataggtg caacagtact tgcaaatgct tgtggaccat gcattggtca
     2581 atggcataga cctgaactca aacagggaga acgaaatact atagtaacat catacaatcg
     2641 taattttccc ggtaggaatg acggtaaacg agaaacaatg aatttcattg caagtcctga
     2701 attggtaact gccttagcac ttggcggtaa tctttctttt aacccattaa atgattctct
     2761 tgtagcatct gacggaacaa agtttagact ggatccgcca gaaagagcgc cagatgtgcc
     2821 tgatagagaa tttgtaaatg tagaagatat ttacttgtct ccttcagaaa atcctgacac
     2881 tgttcaagtt ttgattgatc cagaaagtga aagattacaa aaacttgaac cattcactgc
     2941 atgggacggt caggatttga ataacctgca tataattatg aaagcaaaag gaaaatgtac
     3001 tacagatcat atctcacctg ctggtccttg gttaaggctt agaggtcact tggataaatt
     3061 aagtgataat ttgttgctgg gtgcagtcaa tgcatttaat gatgaagtgg gtaaagccaa
     3121 aaacttactc acaaataaaa ttgagaactg tgcagaaatt gcgagacagt ataaaaaaca
     3181 aaatatttct tggattataa ttggcgatga taattatggt gaaggaagca gtcgtgagca
     3241 tgctgcaatg acaccacgtt atttgggatg taaggtggtt attgcaaaaa gctttgcacg
     3301 tatacatgaa acaaatctca agaagcaggg ggtaatggca ttgacatttg ataaccctaa
     3361 agattatgaa aaaattcatg aagatgataa aattagtata aactctttga gtgatatttc
     3421 tcctgggaag tcagttgatt gtactgtaga acatgttgat aaaacatctg aaaagattat
     3481 cttaaatcat tcttacaatg atttacaaat tgaatggttc agagcaggtt ctgccatgaa
     3541 ctttttaaga aaaaaccaaa aggattaatc ttaacgtggg gccgatcgga attgaaccga
     3601 cgacctacgg gttacttcgc agctccaaac tcacatcatc ttttcatcag agtttgaact
     3661 ttaa
//
LOCUS       DASCPJ010000039         4473 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000742627, whole genome shotgun sequence.
ACCESSION   DASCPJ010000039 DASCPJ010000000
VERSION     DASCPJ010000039.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000742627"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="tuf"
                     /locus_tag="QGF01_01970"
     CDS             complement(<1..1117)
                     /gene="tuf"
                     /locus_tag="QGF01_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HJN19698.1"
                     /translation="MADKDHLNLIVTGHIDNGKSTTMGHFLMDLGIVDERTIAAHAKE
                     SEETGKGDTFKYAWVMDNIKDERARGITIDLAFQKFETPKYFFTLIDAPGHRDFVKNM
                     ITGASEADCAVLVLSAKEGECDTAIAPGGQAREHAFLLKTLGVAQIIVAVNKMDAADY
                     KEEAYNAVVQKGTELVKQCGYKPDTVPFIPVSGWKGDNLVKKSENMAWWKGDTLLESF
                     DKFTVPEKPTGKPLRLPIQDVYSITGVGTVPVGRVETGVMKAGDKIVVMPAGATGEIK
                     SIETHHTEMPSAQAGDNIGFNLRGIEKKDIKRGDVLGDPTNPPMIAKEFRAQIIVIHH
                     PTAIAPGYTPVMHCHTAQVAATITAFESKIDPATGGTA"
     gene            1253..2389
                     /gene="fbp"
                     /locus_tag="QGF01_01975"
     CDS             1253..2389
                     /gene="fbp"
                     /locus_tag="QGF01_01975"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HJN19699.1"
                     /translation="MKITVSVIKADVGGIGGHTRPSDRLLDAIRNTVKNSGDLLIDHY
                     IGYCGDDSHIVMTHNHGVDNQKIHQLAWDAFMAGTEIAKQEGLYGAGQDLLKDSFSGN
                     IKGMGPGVAEMEFEERLNEVFTVFAADKTEPGAFNYPIYKLFVDSLSNTGLIVNQSLA
                     KGVNINIMDVEEGKIANLSLWEDKPLIEAALMYPSRYVVDSVYTKDGEPILDASTDRL
                     HNIAGTYVGKDDPICLVRTQKNFPATEEAGSVFSNPHYVAGNTRGSHNMPLMPVKLNS
                     AATINFCIPIVEALVFSMHNGKLTGPFDGFSTPDWDYIREIATKKALTMRSQGFIHPA
                     TLVPSELEYAEGYKAVMDVLHAKMKPIEDQPHDNQRKESYEDPD"
     gene            2433..2843
                     /locus_tag="QGF01_01980"
     CDS             2433..2843
                     /locus_tag="QGF01_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19700.1"
                     /translation="MKSPKIISGLKPFAITYISVIAFSNFVFVLFSQTVRDIVWSFFK
                     DAGGVIILGMVFLFALTWLIKARPQNIPKKYQIIVFDVFGKESHVDGMRTEFKNHDVA
                     WSFMKFYKKSYPLCNFAMVTKQKDSAKKIIFKYI"
     gene            2908..>4473
                     /locus_tag="QGF01_01985"
     CDS             2908..>4473
                     /locus_tag="QGF01_01985"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HJN19701.1"
                     /translation="MEFSTLSEIFQRMEKTSKRIELTDILVELLKKTPKEILPNVVYL
                     LQGIIRPNFEGVELGIAEKLAIHAISKSSGLSIKKIEDDYREGGDLGLTASNILKLKT
                     QTTFTVEKITVERVYDTLFKIAKLEGKGSQDLKMKYISSMLNDATPTEAKFVMKILLG
                     TLRLGVAENTVMDALAIAFTGKKENKEEIENAYNVSSDLGKVSLIVATDGIEEIKKFK
                     ISLFSPIRPMLADRVKSETDAIKKMPQLFAAEYKLDGERVQIHKQMNKIILFSRRLEN
                     ITQYYPDIIENIGKYINVDEGIFEAEIVPINENTGEFLPFQELMHRRRKYKLEKAVSQ
                     YPITVNFFDVLYFDDRNCLNLEYTKRREILEKVVKEDNFAKLVPMLLVKNENEIEDFL
                     ENSINSGCEGLMLKTPNAVYRAGARGSNWLKLKREYRNELGDSLDLIVIGAYFGRGRR
                     TGLYGTLLLGTYNPENDTFPSICKVGTGFTDESLDQLYQILSNKVILKKNSRVVSEME
                     ADVWLEPELVLEIV"
BASE COUNT         1528 a          689 c          825 g         1431 t
ORIGIN      
        1 ctgctgttcc accagtagca ggatctattt tagattcaaa tgcagtaatt gttgcagcca
       61 cttgtgcagt gtgacagtgc attactggtg tataaccagg tgcgattgct gttgggtgat
      121 gaatgacgat tatttgtgcc ctgaattctt ttgcaatcat tggtgggttt gtaggatcac
      181 caagaacatc tccacgtttg atatccttct tttcaattcc tcttaagtta aaaccaatat
      241 tatctcctgc ttgagcagat ggcatttctg tatgatgagt ttctatagat tttatttcac
      301 cagttgcacc tgcaggcata actacaattt tatcaccagc tttcattaca ccggtctcaa
      361 cccgtcctac aggaacagtt cctacaccag taattgaata aacatcttgg ataggaagac
      421 gtaatggttt tcctgtaggt ttttcaggaa cagtaaattt atcaaatgat tctaataatg
      481 tgtcaccttt ccaccatgcc atgttttctg attttttcac gagattatca cccttccagc
      541 ctgaaactgg aataaatgga actgtatcag gtttgtaacc acattgtttt actaattctg
      601 tacctttttg tacaactgca ttatatgctt cctctttgta atctgccgca tccattttat
      661 ttacagcgac tataatctga gcaacaccta aggttttcag taaaaatgca tgttctctag
      721 cttgaccacc aggtgctata gcagtatcac attctccttc ttttgctgaa agaaccaaaa
      781 cagcacaatc tgcttcagat gcaccagtaa tcatgttttt tacaaagtct ctatgacctg
      841 gtgcgtcaat taatgtgaaa aagtattttg gtgtttcaaa tttctgaaat gcgagatcga
      901 tcgtaattcc tcttgctctt tcatctttaa tgttatccat aacccaagca tacttgaatg
      961 tgtcaccttt tccagtttcc tcagattctt tggcatgtgc agcaattgtt ctttcatcaa
     1021 ctataccaag atccatcaaa aagtgaccca tagtagttga tttaccatta tctatgtgtc
     1081 ctgttacaat tagattcaaa tggtctttgt ctgccatatt gttggcaaac tgagacgggc
     1141 tttattacta tttcgatttt ttgtaaaaat tagttatatc ttctcattct tttttatttt
     1201 tgaaatatgg atgatgcata tagtataaat cgaggttata gtatgaaaat tcatgaaaat
     1261 caccgtatca gtaatcaaag cagatgtagg tggtattggc ggtcatacaa gaccaagtga
     1321 tagattactc gatgcaatca gaaatacagt aaaaaactct ggtgatctac tgattgatca
     1381 ctatattgga tattgtggtg atgattccca cattgtaatg actcataatc atggtgttga
     1441 caaccaaaaa atacaccaat tagcatggga tgcatttatg gctggtactg aaattgctaa
     1501 gcaggaaggg ttgtatggtg caggacaaga tctgttgaag gattcatttt caggtaatat
     1561 caaaggcatg ggcccaggtg tagcagaaat ggaatttgaa gaaagattaa acgaagtatt
     1621 tactgtattt gctgctgata aaactgaacc cggtgctttc aattatccga tctataagtt
     1681 atttgttgac tcacttagta atactggatt aatagtgaac caatcactcg caaaaggtgt
     1741 caacataaac ataatggatg ttgaggaagg taaaattgcg aatctttcat tatgggaaga
     1801 taagccattg attgaagctg ctcttatgta tcctagtcgt tatgtggttg attcagttta
     1861 tacaaaagat ggtgaaccga tattagatgc gtccactgat aggttacata acatagctgg
     1921 cacttatgtt ggtaaagatg atccgatttg tctagttaga acacaaaaaa actttccggc
     1981 tactgaagaa gcgggaagtg tatttagtaa tccacactat gtggctggaa acactagagg
     2041 cagtcataat atgcctttaa tgccagttaa acttaattcg gccgcaacga tcaatttttg
     2101 tattccaatt gttgaagcat tagttttcag tatgcataat ggtaaactaa caggtccatt
     2161 tgatggattc tcaacacctg attgggatta catacgtgaa attgctacaa aaaaagcatt
     2221 aacaatgaga agtcaaggat ttatccatcc agctacacta gtaccatcag agttagaata
     2281 tgctgaagga tacaaagctg taatggatgt tttacatgcc aaaatgaaac caatagaaga
     2341 tcagccacat gataatcaaa gaaaggaatc ttatgaagat cccgattaga ttaatcaaaa
     2401 aaggttaaat caaattaaaa ataaatacaa ttgtgaaatc acctaaaatc attagtgggt
     2461 taaaaccatt tgctataaca tacatttccg tcatagcttt ttcaaatttt gtttttgtat
     2521 tattcagtca aactgttcgg gatattgttt ggtcattttt taaagatgct ggaggagtaa
     2581 taatacttgg aatggtgttt ctatttgcac ttacatggct gattaaagca agaccacaaa
     2641 atattccaaa aaaataccaa attatagttt ttgatgtatt cgggaaagaa tcacatgtgg
     2701 atggaatgag aactgaattt aagaatcatg atgtcgcatg gagttttatg aaattttaca
     2761 aaaaatcata cccactatgc aactttgcta tggtcacaaa acaaaaagac tctgctaaaa
     2821 aaataatttt caaatatatc taggtctatc acacccattc tcaattttag gttcaggttt
     2881 attacttgaa tacgcaaaaa aaatctaatg gaattttcaa ctctttccga aatatttcag
     2941 aggatggaga aaacatcaaa gcgtatagaa ctgacagata ttttagtaga attattaaaa
     3001 aaaacaccta aagagatact cccaaatgtt gtttatcttt tacagggcat cattagacca
     3061 aattttgagg gtgtagaatt aggaattgct gaaaaacttg caattcatgc tatttcaaaa
     3121 tcttctgggt tatcaataaa aaagattgaa gatgattata gagaaggagg tgatctaggt
     3181 ctaactgctt caaacatatt aaaattaaaa acccaaacca catttactgt agaaaaaatt
     3241 actgtagaaa gagtttatga tacattattc aagattgcaa agttagaagg taaaggttca
     3301 caggatttga aaatgaaata catttcaagc atgctaaatg atgcaacccc aacagaagca
     3361 aaatttgtca tgaagatttt gttgggtaca ttacgtttag gagtagcaga aaatactgtc
     3421 atggatgcac ttgctattgc ttttactgga aaaaaagaga ataaggaaga aatagaaaat
     3481 gcatataatg tttcaagtga tttaggtaaa gtttctttga ttgttgcaac agatggaatt
     3541 gaagaaataa aaaaatttaa gatttcatta ttcagcccaa taagacctat gctggcagat
     3601 agggtaaaaa gtgaaacaga tgcaattaaa aaaatgcctc agttatttgc tgcggaatat
     3661 aaattggatg gagagcgtgt gcaaattcat aaacagatga ataaaataat tttattttca
     3721 agaagactag aaaatattac acaatattat ccagacatta tagaaaatat tggaaaatat
     3781 attaatgtgg atgaaggaat ttttgaagca gaaattgttc ctattaatga aaatactggt
     3841 gaattcttac catttcaaga gctgatgcat agacgaagga aatacaaact tgagaaagct
     3901 gtttcacaat atccaataac agttaatttt tttgatgtcc tatattttga tgatagaaac
     3961 tgtttaaatt tagaatacac taaaagaaga gagattttag aaaaagtagt taaagaagat
     4021 aactttgcaa aacttgtacc tatgctgctt gttaaaaatg aaaatgagat tgaagatttt
     4081 ctagaaaact ctatcaattc tggttgtgaa gggttaatgc tgaaaactcc taatgctgta
     4141 taccgtgctg gagcaagagg tagtaattgg ttaaaattaa aacgtgaata tagaaatgaa
     4201 ttaggagata gtcttgatct aattgttata ggtgcatatt ttggaagagg gcgtagaact
     4261 ggtctatatg gcacattgtt attgggaacg tataatccag agaatgacac ttttcctagt
     4321 atatgtaaag ttggcactgg ttttactgat gaaagtttgg atcagttgta tcaaattctc
     4381 tcaaacaaag tcatacttaa aaaaaattct agggttgtaa gtgaaatgga agctgatgtg
     4441 tggctcgaac ctgaattagt attagaaatt gtt
//
LOCUS       DASCPJ010000040         4087 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000746762, whole genome shotgun sequence.
ACCESSION   DASCPJ010000040 DASCPJ010000000
VERSION     DASCPJ010000040.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000746762"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="thiC"
                     /locus_tag="QGF01_01990"
     CDS             complement(<1..1009)
                     /gene="thiC"
                     /locus_tag="QGF01_01990"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HJN19702.1"
                     /translation="MATQMSSARRGVATDEMKQVAKDEDVTLDWLLPKIASGSIIIPS
                     NNTRPQKIHNVGIGKGMKTKVNVNIGTSTLNVNIEEEIEKAKVAVKYHADTIMDLSDG
                     GDVGEIRRALLDVAPITFGTVPIYEAYNFGVEKHKNPLDITEDDYLKAFENNIKDGVD
                     YTTIHSGITKEIAKRILKVQRYAGIVSKGGTITAAWMLKYDKENPYITNYDYMIELAQ
                     KYDVTFSLGDALRPGSILDSHDELQVQEMINIGRLAKRAIEKDVQVMVEGPGHVPLDE
                     VATNVKLAKSMIGDVPYYVLGPLVTDVASGHDHIASAIGAAVSASEGVDLLCYLTPSE
                     HL"
     gene            1093..2376
                     /gene="thiD"
                     /locus_tag="QGF01_01995"
     CDS             1093..2376
                     /gene="thiD"
                     /locus_tag="QGF01_01995"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HJN19703.1"
                     /translation="MNILSIGGSDPSSGAGIQSDIKTFSNYNVYGFTIITAITSQNTR
                     KVTSIEPVSTKILIAQLDSILSDFHIDAIKIGMVYNSRIVKAIYSRLKNIKVPIIIDP
                     IIKSTTGTILLKKNAVDDYKKMIIPLADVITPNKYEAKVLSGLSNIKRSAKKIQDMGA
                     KCVIITSSTESSTKISDYILEKNKDYLISGKKIPIRNHGSGCNYSAVITVSLAKGNTI
                     NHAVKMAKDYTYQSIKNAKNIGKGVSITHKNIPSRMQVLTDSINDFKQIKNIYKIIPE
                     CQTNFVFAKKNPKTIKDVLGISGRLVKSGKEIITAGEIIYGGSQHVATAVIQVSKKFP
                     EICSGLNIKYDPKIISKAKKIGFIVLNYDRSKESKKSRASENTSISWGISNSLKTKLP
                     DVIYHKGDIGKEPMILIFGKDPRDVIRKTSILTTY"
     gene            2425..3123
                     /locus_tag="QGF01_02000"
     CDS             2425..3123
                     /locus_tag="QGF01_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HJN19704.1"
                     /translation="MKAVIIAGGLGVRLRPLTNKKPKPMLPLDKKPLLEHLIKWIKKN
                     GIKDIVLCVSYLHETIEKYFEDGSKFGVKIEYAVSKKPLATAGQLKTAQKFIDDTFVC
                     VYGDTIFDFNLKNMINEHKKKKSFVTMGLYEYKTHLKYGVIDTKNSGKVSAWNEKPEI
                     KVKINMGCYVMEPKILNFIPKNKPYGMDSVIKKLIADRFAINSVLVKKGFIDVGDKKT
                     YEKLRLEYRKRGKI"
     gene            3130..3567
                     /locus_tag="QGF01_02005"
     CDS             3130..3567
                     /locus_tag="QGF01_02005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJN19705.1"
                     /translation="MSEIEIREIDESDLERGFLESLDFLRKASDIDKNKAYDILRKIK
                     QNPDHIIHVALDNNKIVGSTTLFIEQKFIHNGGRVGHIEDVVIRKDYEGRGIGMKLVI
                     SLLNRAKEEKCYKTILDCKDDLKIFYERIGFKSESNCMRYNHD"
     gene            complement(3564..4022)
                     /locus_tag="QGF01_02010"
     CDS             complement(3564..4022)
                     /locus_tag="QGF01_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19706.1"
                     /translation="MALQYGAYIAMAGIALYAIFAGEMISILNYMLEPAVEQTLENDF
                     LKPRVYDAPSKILQFISIGVAPGLVMSGTSYMLARKFGSKQIGWLIIIGGLVLLIGMF
                     YSYTMLDDIEKDYQIFTVTITPPLFMAVSIPIMITGSLLFRIKKRPKKYF"
BASE COUNT         1490 a          642 c          666 g         1289 t
ORIGIN      
        1 ctaaatgttc agatggagtt agatagcata aaagatccac cccctcactg gctgaaactg
       61 cggcacctat tgcgcttgca atatgatcat gcccagaggc aacatctgta accaatggtc
      121 caagaacata atatggaaca tcaccaatca tagattttgc gagtttaaca tttgttgcta
      181 cttcatcaag tggaacatga cctggacctt caaccatcac ttgtacatct ttttcaattg
      241 ctcgttttgc taatcgtcca atgttaatca tttcctgcac ttgtaactca tcatgtgaat
      301 ctaatattga accaggacgt aacgcatcgc ctaaactgaa ggtaacatca tatttttgtg
      361 ctaattcaat catgtaatcg taattagtga tatagggatt ttccttatca tactttagca
      421 tccatgctgc agttattgtt cctcctttac ttacaatacc ggcataccgc tgtaccttga
      481 gtattctttt tgcaatttct tttgtaattc cagaatgaat tgttgtataa tcaactccat
      541 cctttatgtt attttcaaat gcctttaggt aatcatcttc tgtaatatca agtggatttt
      601 tatgtttttc aaccccaaaa ttatatgctt cataaattgg tacagttcca aatgttatag
      661 gtgcaacatc taataacgct cttctaattt caccaacatc gccaccatca ctaagatcca
      721 ttattgtgtc tgcatggtat tttacagcaa ccttagcttt ttcaatttcc tcttcaatat
      781 tgacattcag tgtagatgtt ccaatgttaa cattaacttt ggttttcata ccttttccaa
      841 tccctacgtt gtgaattttt tgaggccttg tattgttact tggtattatg attgaaccac
      901 tagcaatctt tggtagaagc caatccagtg taacatcctc atcttttgca acctgcttca
      961 tctcgtcagt tgcaactcct cttcttgcag agctcatttg agtagccatg aatataataa
     1021 ccaacaactg aatttaaaca atcatcatat cagcgatcaa attttaaaac atggcatata
     1081 gattagtatg atatgaacat tctttcgata ggtggatctg acccatcatc cggtgctggt
     1141 atccaaagtg acatcaagac gttttccaac tataatgttt atggttttac catcattact
     1201 gcaataacaa gtcagaatac aagaaaagtg acttcaattg agcctgtttc tactaaaata
     1261 ttgatagcac aactggattc aattttatct gattttcata ttgatgcaat caaaattgga
     1321 atggtttaca actcaaggat tgtaaaggct atttattcaa gattaaaaaa tatcaaagtt
     1381 ccaataataa tagatccaat aataaaatca acaactggta caatacttct gaaaaaaaat
     1441 gcagttgatg attacaaaaa gatgatcatt ccgttagcgg atgtaataac tccaaataaa
     1501 tacgaggcaa aagttttgtc tggactgtca aacataaaaa gatctgcaaa aaaaattcaa
     1561 gatatgggtg ccaagtgtgt aatcattact agttcgactg aatcgagcac taaaatctct
     1621 gattatattt tagaaaaaaa caaggactac ctaatttctg gtaaaaaaat tcctattaga
     1681 aatcatggaa gtggatgtaa ttactctgcg gttattacag tgtcacttgc aaaaggaaat
     1741 acaattaatc atgctgtaaa aatggctaag gactatactt atcaatccat aaaaaatgca
     1801 aaaaatatag gaaagggggt gagtataacc cataaaaata ttcccagtag aatgcaggtt
     1861 ttaacagatt caataaatga cttcaaacaa atcaaaaata tttacaaaat aattccagag
     1921 tgtcaaacaa attttgtgtt tgctaaaaaa aatccaaaaa ccataaaaga tgtattaggt
     1981 atatcaggcc gtttggtaaa atcaggtaag gagattataa cagctggtga aattatctat
     2041 ggtggttcac agcatgttgc tacagctgta attcaagtaa gtaaaaaatt tccagaaatt
     2101 tgttctggtc ttaacataaa atatgatcca aaaattattt caaaagcaaa aaaaattgga
     2161 ttcattgttt taaattatga tagaagtaag gagtcaaaaa aatcaagagc gagtgaaaat
     2221 acttctattt cttggggaat atctaattcg cttaaaacaa agttacctga tgtgatttat
     2281 cacaaaggtg atattggaaa agaaccaatg attctaatat ttggtaaaga cccacgtgat
     2341 gtaatcagaa agacctctat tctaactact tactgaaaat tgtataactt aacataaata
     2401 atgctacaac gagaatacaa attcatgaaa gcggtaatta ttgctggagg attgggtgtt
     2461 agactacgtc cacttactaa taaaaagcca aaaccaatgc tgccattaga caagaagcca
     2521 ttattggaac acctaatcaa atggattaaa aaaaatggta taaaggatat tgttctatgt
     2581 gtgagttacc tgcatgaaac aattgaaaaa tattttgagg atggtagtaa atttggtgtg
     2641 aagattgaat atgccgtatc caaaaaacca cttgctactg caggacaact aaaaactgca
     2701 caaaagttca tcgatgatac atttgtttgt gtttatggcg acacgatttt tgattttaac
     2761 ttaaaaaata tgattaatga gcataagaaa aaaaaatctt ttgttacaat gggcctttac
     2821 gagtataaaa ctcatttaaa gtatggagtg attgatacaa aaaatagtgg taaagtatct
     2881 gcatggaatg aaaaaccaga aattaaggta aaaatcaata tgggttgtta tgttatggag
     2941 ccaaaaattc taaattttat tcctaaaaac aaaccatatg gaatggatag tgtaataaaa
     3001 aaattaattg cggatcgttt tgcgattaac agcgtactag taaaaaaggg attcatagat
     3061 gttggtgaca aaaaaaccta tgaaaaatta agattagagt acagaaaacg aggaaaaatt
     3121 tgatttaata tgtcagaaat tgaaattcgt gaaattgacg aaagtgatct tgaaagaggg
     3181 tttctagaaa gtttagattt tttaagaaaa gctagtgata ttgataaaaa taaagcatac
     3241 gatattttaa gaaaaattaa acaaaatcca gatcatataa tccatgttgc actagataat
     3301 aacaaaattg ttggatcaac tactttgttt attgagcaaa aatttatcca taatggtgga
     3361 cgagttggtc atattgaaga tgttgttatc agaaaagatt acgaaggacg tggaatcggt
     3421 atgaaattag tcatatcatt attgaaccgt gcaaaagagg aaaaatgcta caaaacaatt
     3481 ttagactgta aagatgatct aaagatattt tatgaaagaa ttggatttaa atctgaatca
     3541 aattgtatgc gatacaatca tgattaaaaa tatttttttg gtcgtttttt gatacgaaat
     3601 aaaagtgagc ctgtaatcat aattggtatt gatacagcca tgaataatgg tggtgttatc
     3661 gtaacagtga aaatttgata atctttttct atatcgtcta acattgtgta tgaataaaac
     3721 ataccaatca acaatactaa accgccaatg attatcagcc atccaatttg ctttgaacca
     3781 aattttcttg caagcatata tgatgtgcca gacataacta atccaggagc aacacctatt
     3841 gagataaact gtaaaatttt ggatggtgcg tcatagaccc gtggttttaa aaaatcattt
     3901 tctaatgtct gctctactgc aggttctagc atatagttaa gaattgaaat catttctcct
     3961 gcaaatattg catataacgc aatacctgcc attgcaatgt aagcgccata ctgtaatgcc
     4021 atatgaatca gattctgatg tgtataaata aactatattt attcaatacc tacaagatta
     4081 gcaagtt
//
LOCUS       DASCPJ010000041         7401 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000750752, whole genome shotgun sequence.
ACCESSION   DASCPJ010000041 DASCPJ010000000
VERSION     DASCPJ010000041.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000750752"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="QGF01_02015"
     CDS             complement(<1..1104)
                     /locus_tag="QGF01_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19707.1"
                     /translation="MSDIFMKIFQAKFFRFQADIIPEKGPWAALILIPVTIISGAYWT
                     SIPFYGVIAVLWLFQRKTPIQWEAGLSLMGITLLGVFVVSKTDYAFWIYTIKIIGCFV
                     IVTSLRPAIQAHQRIWVDIYFVLLLGIIWETNAYIHYGRWTFDMGFGRYLPNWTAIYC
                     FLFAMLAVRWRLPLTLLVTLTFGWLTGSRNFALALGVVGMGYLLFPIWQWVFSKTSRT
                     VVLYLFSAGLALYILEQITKGQWTGGFFFNERILKFTADAGRLNWDMIGISEWTKSMQ
                     NFAWGVGDWPNDGPNPHHSLIHTLLERGIVGTGVLIVFIMNDLSKHARGMEPWLLGWF
                     SLGMYLHLNFVTMFWVISILILRCRYVRNGKDIQ"
     gene            complement(1463..1843)
                     /locus_tag="QGF01_02020"
     CDS             complement(1463..1843)
                     /locus_tag="QGF01_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJN19708.1"
                     /translation="MIEFISNYFEHSDERGSFQGLVNFGNWEEINIISSEAHSIRGNH
                     YHKYTDELFIILEGRIKITLQNVLTNKKLGKKKKTFEVLKGDVFFIKKNVNHIFETIE
                     FSKWINVLSMKTNKKKPDINRINK"
     gene            complement(1840..2694)
                     /locus_tag="QGF01_02025"
     CDS             complement(1840..2694)
                     /locus_tag="QGF01_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016228.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HJN19709.1"
                     /translation="MVIAIIGASGFIGNRIFHSLSIKNTFNLIGTFYKHKTSSEFIHL
                     DVTSIEQIKQFLIRYRPSLIFWLAGSKDLNECENDIKYAYRINTQPIIDFYQIKKRIN
                     LDSRLVFFSTDYVFDGVKGNYKDNDVGNPKTNYGIANKLAEDAIRHGSNNDLIIRTSA
                     AMGRGGQFFDWLTKSLVDDKEVKMFNDIFFSPTPIQLLSEATEYLINHRRSGIIHICG
                     GLRLSRFEFAKILLGINKTFSATIVPISASNASNPFQNDLSLIKSNICEKFQTKDFEN
                     YIREELLS"
     gene            complement(2685..4067)
                     /locus_tag="QGF01_02030"
     CDS             complement(2685..4067)
                     /locus_tag="QGF01_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN19710.1"
                     /translation="MKIVFVNPPNIRSNTSTAKNDFTIKEFVVPKFLSKIKWSEKVFK
                     GLEHITGLGNGVRYGVRAGSRWPFTMDQPLSNYAPYPFFMGYAASYLMENGFEVNILD
                     AVAEMEYNYDTFLEEIKLEEADIVVLECSTPTIDIDVWFANKIATFSKVALAGPHLNR
                     TTVTEIMPDNPKINFYLLGEYILSSLKMAKSQKNGIYDSEILKNIDSITPPYRDYKSA
                     SKYFDPSMPTPKPQLAIYGSKGCPFKCTFCAWPQTMYFGNVALRSPDNIAKEIRDAVD
                     IYGFRSIFFDDDTFNLGTKRVSKLCDYLKEINIPWTMMGRLDISPDWLYDKMVDSGCV
                     GMRFGVETFNIDVLKNIEKGIERRDFRKTLEYICSKYPEIMIHITMMSDMPGQTDADT
                     EMDMKIIKEMGFTTDNILRSYQLSKCAPFPGTKLYEQMKTKLDEPTLKTYKAYDGGQD
                     TVMKVSKKWS"
     gene            complement(4064..5041)
                     /locus_tag="QGF01_02035"
     CDS             complement(4064..5041)
                     /locus_tag="QGF01_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJN19711.1"
                     /translation="MDIGFYIIWNKHSVKSKGNVVGDELFAESLCKSINKQFKSINAE
                     LYAPNYLPKKKLDALIYLNESAPNEKLSKYHFLYIQNGCEENIDIIINKSIALGYDGH
                     IFFSEIIKNLFDKKKQPRKNSLYLPFGVDLDHFSPRQRENRFTFDCAFIGNDIKGPES
                     AMKYLYPAAEFNFGLFGNWKVERHKYKIWKNLKKLPPYKKVFENLSRGKISQNDVPIL
                     YSSAKINLNNTIKSCVEWDVITLRTYEVLACKGFLISDSVPIAKKTLKDCMVFTEGGS
                     DLTEKIEYYLKYPKMREEMAQNGYNFVVKYCSIDSRVGELINYVQEIVK"
     gene            complement(5085..6398)
                     /locus_tag="QGF01_02040"
     CDS             complement(5085..6398)
                     /locus_tag="QGF01_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005498153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HJN19712.1"
                     /translation="MAKTGFKRYFENTSWLIAEKITRLTVGLFVGIWIARYLGPEQFG
                     TLSYAQAFVGLFGAISTLGLDGIVIEILVKDEDNRDLYLGTTFVLKLLSAIVVMVIIC
                     FVALITSNDNNDAFIITIIASTLLFQSLNVIDFYFQSKVLSRYIVIANLTSQLIVSLV
                     RVVLILTNASIIAFAYVILLDAIILALGYIYVYKHKNLSFRNWKFKNTTAHDLLKLSW
                     PLALSGIVISIYMKIDQVMIKEILGKTSVGQYAAAVKISEAWYFVPVAISSSLFPAII
                     NAKKRSKKEFYDRVKMLYSFMFWIAVSVSIPLSFYSNNIIVLLFKKQFDLAGSVLMIH
                     IWAGVLVSLGVVTSNIVISNKTQKNALIATCIGAFSNIILNFFLIPSYGIIGAAFATI
                     LSQLISGIFVPIVYKIDPFYPNILRSSLLYLPRELFANWNRNQSK"
     gene            complement(6557..>7401)
                     /locus_tag="QGF01_02045"
     CDS             complement(6557..>7401)
                     /locus_tag="QGF01_02045"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HJN19713.1"
                     /translation="RLLPVADRLHLHSGTLESFPALYKIFRDVQPDECYHLAAQSFVS
                     ISFEDEFSTMQTNINGTHYLLAALKDSYPECRFYFAGSSEMFGKVEETPQCETTRFHP
                     RSVYGITKVAGFELTRNYREAYGLFTCTGMLFNHESPRRGFEFVTRKITSTAARIKLG
                     LEKELRLGNVEAQRDWGFSGEYVKIIHIMLQQDEPDDFVIGTGQTHTVREFVQIVFDE
                     LELNYEDHLVIDPRFYRPAEVEILVANPAKAREKLGWEPKVKFKELALSMVRADYDIL
                     MKNK"
BASE COUNT         2379 a         1371 c         1166 g         2485 t
ORIGIN      
        1 ctgaatatct ttaccatttc ttacataacg acagcgcaaa ataagtatgg aaattaccca
       61 gaacatggtc acaaaattta aatgcaaata cattccaagc gaaaaccatc ccaaaagcca
      121 tggctccatt ccgcgagcat gtttgcttaa atcattcatg ataaaaacaa tcaatacacc
      181 tgtgcccaca attccccgct ccaaaagggt gtgtatcaga gaatgatggg gattagggcc
      241 atcgtttggc caatctccca ctccccacgc aaaattctgc atgctctttg tccattcact
      301 aattcctatc atatcccaat tgagtcgtcc agcgtctgca gtgaatttta atattcgctc
      361 attaaagaaa aagccccctg tccattgtcc tttagtgatt tgctccagta tataaagtgc
      421 taaccctgcg gaaaacaggt acagtactac tgtccgactg gttttggaaa aaacccactg
      481 ccagatgggg aataaaagat aacccattcc tacgacacca agagcgagag caaaatttct
      541 acttcctgtc agccaaccaa atgtcaatgt tactagcaga gttaagggta acctccagcg
      601 aacagcgagc attgcaaata aaaaacagta tattgccgtc cagtttggga gatatcttcc
      661 gaatcccatg tcaaatgtcc atctgccata atgaatgtat gcatttgttt cccaaataat
      721 ccctagtaac aacacaaaat agatatcaac ccaaatccgt tgatgagctt gtattgcagg
      781 tcttaacgaa gttacaataa caaaacagcc tatgattttg atagtgtaaa tccaaaaagc
      841 ataatctgtt tttgacacaa caaaaactcc taacagagta atacccatta gagagagccc
      901 tgcttcccat tgaatgggag tcttgcgttg aaaaagccaa agcaccgcta ttactccata
      961 aaaagggatt gaagtccagt aggctccaga gattattgtc actggaatta gtattagggc
     1021 cgcccaaggg cctttttcag gtatgatgtc tgcttgaaaa cgaaaaaatt ttgcttgaaa
     1081 gattttcata aaaatatcac tcattttttt catttctaca ctgaccactg ttggtgatga
     1141 tttgttcgaa atgttctaag tttttgaaga ttcattagtt tttaacaatt cttcataatt
     1201 attgagagtt ttagtaatat gatatattag gtttatgcca ttcataattt atttgaagga
     1261 aactactaat atcaaaaaac tcataactga aaaattgttt gatggtttgg tatgtacctc
     1321 tcttcgtcaa ttatttttgc caaagagcta aacataatga aatgattgag ttcaaaaatg
     1381 tttactttta tctcgaagca aggatgctta cttaggaaat cctgaaaaat cttttcagga
     1441 taggtgaaaa agggagaaaa atttacttat tgattctatt gatatcaggt ttttttttgt
     1501 tggttttcat tgaaaggaca tttatccact ttgaaaactc aattgtttca aatatatgat
     1561 tcacattttt ctttataaaa aaaacatctc cctttaggac ttcaaatgtt ttcttttttt
     1621 tacctagttt tttgttagta agaacatttt gcaaggttat ttttattctt ccttctaata
     1681 taataaacaa ttcatctgta tatttatgat agtgatttcc cctgatacta tgagcttcgg
     1741 aggatataat atttatttct tcccaatttc caaaatttac caaaccctga aaagaacctc
     1801 tctcatcaga atgctcaaaa taattagaaa taaattcgat catgaaagta attcttcacg
     1861 aatataattt tcaaaatctt ttgtttgaaa tttttcgcaa atattagatt taatcagaga
     1921 taagtcgttt tgaaaagggt tgctagcatt tgaagcagaa attgggacta tggtcgcaga
     1981 aaatgttttg ttgataccca acaaaatttt ggcaaattca aacctgctaa gtctaagacc
     2041 tccgcaaata tgaataattc cagacctccg atgattgata agatattccg tagcttcaga
     2101 taataactga attggtgttg gagaaaaaaa aatatcgtta aacattttta cttctttgtc
     2161 atcaactaaa gattttgtca accaatcaaa aaactggcct ccccttccca ttgcggctga
     2221 agttctaata atcaaatcat tattacttcc atgacgtatt gcatcttcag ctaatttatt
     2281 ggcaatacca tagttggttt tgggatttcc cacatcgtta tctttataat ttccctttac
     2341 tccatcgaac acataatccg tagagaaaaa aactaatctt gaatctaaat ttattctttt
     2401 ttttatttgg taaaagtcaa ttataggctg tgtatttatt cgatatgcat atttaatatc
     2461 attttcacat tcatttaagt cttttgaacc agctaaccaa aaaattaaac ttggcctata
     2521 tcgaatcaaa aattgtttaa tttgttctat agaagtaaca tctaggtgaa taaactcgga
     2581 tgaagtttta tgtttgtaaa aggttccaat taaattaaat gtatttttga tgctcaatga
     2641 gtgaaaaatt cgattaccta taaaaccaga agctccaatt attgctatga ccattttttt
     2701 gaaactttca tcacagtgtc ttgaccacca tcatatgctt tataagtttt taacgtaggc
     2761 tcatcgagtt tggttttcat ttgttcgtag agttttgttc cagggaatgg agcacattta
     2821 gataattgat aacttcttag tatattgtca gttgtgaaac ccatttcttt aataattttc
     2881 atatccattt cggtgtctgc atcggtctgt cctggcatat cactcatcat tgtaatatga
     2941 atcataattt ccggatattt agaacatatg tattccagag tctttctaaa atctctgcgc
     3001 tcaattccct tttcaatatt ttttaacaca tcaatattaa aagtttcaac gccaaacctc
     3061 attcctacac atccgctgtc aaccatcttg tcatatagcc agtctggtga aatatcgagt
     3121 ctacccatca ttgtccaggg gatgttaatt tcttttaggt aatcacacaa tttagaaact
     3181 ctttttgtac ctaagttgaa agtgtcatca tcaaaaaata tactcctaaa accataaata
     3241 tcaactgcat cacggatttc cttggcaata ttgtctggac ttcttaatgc aacattacca
     3301 aaatacatag tttgaggcca tgcgcagaaa gtacatttga acgggcaccc tttactccca
     3361 tatatcgcca attgtggctt tggagtgggc atacttgggt caaaatattt tgaggcactt
     3421 ttgtaatctc tatatggtgg agtaatcgaa tcaatatttt ttagaatctc ggagtcatag
     3481 ataccgtttt tttgagactt tgccattttt aatgagctta aaatatattc tccaagcaag
     3541 tagaaattta ttttcgggtt atctggcata atttcggtga cagtagttcg gtttaaatgg
     3601 ggacctgcca gagctacttt agaaaacgtc gcaattttat ttgcaaacca aacgtcaata
     3661 tcaattgtag gagttgaaca ctccaaaact acaatatcag cttcttctag ttttatttcc
     3721 tctagaaaag tgtcataatt atattccatc tctgcaactg catctagaat attaacttca
     3781 aacccgtttt ccatcaagta ggatgcagca tatcccataa aaaaagggta tggtgcataa
     3841 ttcgatagag gctgatccat agtaaaaggc catctagacc cagctctaac tccatatcta
     3901 acaccattac ctaatccagt aatatgttcc aatcctttaa aaactttttc ggaccattta
     3961 attttactta aaaattttgg aacaacgaat tctttaatgg taaaatcatt tttagctgtt
     4021 gaagtattcg atcttatgtt tggcggattt acaaatacta ttttcatttt acaatttcct
     4081 gaacgtagtt aattaactca cctacccttg aatcaattga acagtatttc acaacgaagt
     4141 tatacccatt ttgagccatt tcctctctca tttttggata ttttaagtag tattctattt
     4201 tttcagtaag gtcagatcct ccctcagtaa acaccataca gtctttgagt gtttttttag
     4261 caataggaac tgaatctgaa attaaaaaac ctttacaagc taatacctca taggtcctta
     4321 atgttataac atcccattct acacagcttt ttattgtatt attaaggttt atcttggctg
     4381 aactatacaa tattgggaca tcattttgcg agattttccc tcttgataaa ttttcaaata
     4441 cttttttata tggaggtaat tttttaagat ttttccaaat tttgtattta tgtctttcca
     4501 ctttccaatt accaaaaagg ccaaaattga attctgccgc ggggtacaaa tacttcatgg
     4561 cagattctgg tccctttata tcgttgccaa taaaggcaca atcaaaagtg aaacgatttt
     4621 ctctttgcct tggcgagaag tgatccaaat caacgccaaa aggcaaatat aaactatttt
     4681 ttctaggttg ctttttttta tcaaacagat tttttatgat ttctgagaaa aatatatgtc
     4741 catcatatcc taaagctata cttttattaa taattatatc tatattttct tcgcagccat
     4801 tttgaatgta taaaaaatga tactttgata atttttcgtt aggagctgat tcattcaagt
     4861 atatcaaggc atcgagtttc tttttcggga gataatttgg agcatagagc tcagcgttta
     4921 ttgatttaaa ctgcttatta attgacttgc aaagtgactc ggcaaataat tcatcaccta
     4981 cgacgtttcc tttcgatttg acggaatgtt tgttccaaat tatataaaaa cctatatcca
     5041 ttaatgaata ccgcgaaaat ttttgtaaat actcatttta ttatttattt agattggttt
     5101 cgattccagt tagcgaaaag ttcgcgaggt aaatacaata atgagcttct taaaatattt
     5161 gggtaaaaag ggtcaatttt ataaacaata ggtacaaata tacctgatat tagttgagaa
     5221 agtattgtag caaatgccgc tccaatgatt ccataagatg ggatcagaaa aaaatttaat
     5281 attatatttg aaaaagcacc tatacaagta gctataaggg catttttctg agtcttattt
     5341 gatataacta tatttgaagt aacgacacct aaactaacca aaacacctgc ccaaatgtga
     5401 atcattagta cagagcctgc taggtcaaat tgttttttaa ataataaaac tataatatta
     5461 ttactataga aacttaaagg aattgaaact gatactgcaa tccaaaacat aaagctataa
     5521 agcattttca ctctgtcata aaactctttt ttacttcttt tcttagcatt tattatagct
     5581 ggaaatagtg aagaacttat tgctactgga acaaaatacc atgcttcact tattttgaca
     5641 gcagcagcat attgtcctac agatgtctta cccaatattt ctttaatcat tacttggtct
     5701 attttcatat aaatggagat aactattcca cttaaagcta gtggccaact aagtttcaaa
     5761 agatcatgag cagtggtgtt tttaaatttc caatttctaa acgataaatt cttatgtttg
     5821 taaacatata tgtagccaag tgctagaatt attgcatcaa gcagaatgac ataagcaaaa
     5881 gcaattattg atgcgttcgt taatattaag acaactctta ctaaactaac aattaactgg
     5941 ctggttagat tagctattac tatatatcta ctcaatactt tgctttggaa gtagaaatct
     6001 atgacgttaa ggctttgaaa caataatgtg ctagcaatga tggtgattat aaaagcatca
     6061 ttgttatcat ttgaagttat aagagcgaca aaacatatta tgaccattac aactattgcg
     6121 cttaacaatt taagcacgaa agtagttcct agatataaat cccgattatc ctcatccttt
     6181 acaagaattt ctattacaat tccatcaaga cctagtgttg aaattgcacc aaaaagtccg
     6241 acgaatgcct gggcgtaact taatgtccca aattgctcag gcccaagata tctggcaatc
     6301 catattccca caaagagacc cacggtgagt cttgttattt tttcagcaat tagccaggaa
     6361 gtattttcaa aatatctttt aaagccagtt tttgccaaga tgtttatcat gtaaaatatt
     6421 ggatatttaa ttagaaaaca tttttgagat ttttttcaga gaatttaagg aaatagagtg
     6481 agctatttaa ttcattatca aagattgttg caaatacaat ctcatttgtc tcgatggctg
     6541 acagaattaa gcagaatcac ttatttttca ttaatatatc atagtcagca cgcaccatac
     6601 tgagtgctaa ttctttaaat ttgactttcg gctcccagcc taatttttct ctggcttttg
     6661 caggatttgc caccaggatt tcaacttcag ccggacgata aaatcttgga tcaataacca
     6721 ggtgatcttc ataattcagt tcaagttcat caaagacaat ttgtacaaac tcacgcacgg
     6781 tatgggtttg tccggtaccg atcacgaagt cgtccggctc gtcttgttgt agcataatgt
     6841 gaattatttt cacatattct ccggaaaatc cccaatctct ttgcgcctca acgtttccaa
     6901 gccgcagctc tttttccagt ccaagtttga tacgtgccgc agttgaagtg atttttcggg
     6961 tgacgaactc aaagcctctt ctgggagact catggttaaa cagcattccg gtacaggtga
     7021 ataatccgta ggcttcacga taatttcgtg tcagttcaaa accagctact ttggtaatac
     7081 cgtaaactga acggggatgg aaacgggtgg tttcacattg tggagtttcc tcgactttac
     7141 caaacatttc cgaagaacct gcaaagtaaa atcggcattc tggataagaa tctttgagtg
     7201 ccgccagcag ataatgcgtt ccgttgatgt tcgtctgcat cgttgagaac tcgtcttcaa
     7261 acgaaattga aacaaaactc tgcgccgcaa ggtggtagca ctcatccggc tgaacatccc
     7321 gaaaaatttt gtagagcgcc ggaaagcttt ccaaagttcc ggaatgcaaa tgtaaacggt
     7381 ctgcaaccgg caagaggcgc c
//
LOCUS       DASCPJ010000042         4675 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000752344, whole genome shotgun sequence.
ACCESSION   DASCPJ010000042 DASCPJ010000000
VERSION     DASCPJ010000042.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000752344"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(10..177)
                     /locus_tag="QGF01_02050"
     CDS             complement(10..177)
                     /locus_tag="QGF01_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19714.1"
                     /translation="MKGHRKCSLCNTEMELSYVPMEEWKMDGFLCGRCYSRKLAEFYP
                     GEHVRVEPSEK"
     gene            complement(358..2163)
                     /locus_tag="QGF01_02055"
     CDS             complement(358..2163)
                     /locus_tag="QGF01_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022062.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HJN19715.1"
                     /translation="MGLSHNYPLLDKEKERRMMNLPSRVADIRLFSEEGRLILSEKKP
                     LEGFSRSVEDSIKEATSEATNTVQKGHDYLIWMLTKVFEETVENAENAIIDGPDDCGI
                     DAVLRYEDKKEITLVQSKFGESHNIGMIDKFVKDVERFKNAEQSRIKRDDLSYLWNEI
                     NQKNVKINLVYVTEQYAEYECDVVRVIDLAQTVQILSERKMKPEKGHKATINYLDGYQ
                     RNNIFYCTVKGIEIANLVDRNKYILDNNIRHHLGHRMKVNRGIRKTLEECPERFAEYN
                     NGLTMTCVDFNDLEGKIECDSPIIVNGAQTSHGILDVSKKTKNLDAEIQLKVIKTDDE
                     EQQKSITRFSNSQNAVKGKDLVALEDYWKSISYQIDTRTGYFFEYQSGSWDYRLDAGQ
                     RAKFQGNEIFNQYLPENHKKKLASKDAIQTYVAYFKQNPSGAYQSISKFLPGGAEYEK
                     IFTEELMDDYRIFLFPTLIMECAKNELEYGPKNKTHPFKRYSTIFFVGVTGKIIHEYI
                     LETKDDFDNDISKLESMIRNVSLFKRILRLTDKIVDQVLASRDLGEELEKVNNSPHNL
                     FSKSIYNYEMKKVIEHYISLESEEISNIKKTISGL"
     gene            2177..2302
                     /locus_tag="QGF01_02060"
     CDS             2177..2302
                     /locus_tag="QGF01_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19716.1"
                     /translation="MIREAVEVWMKEMDVTYHETLTEKKTRGRPRIKRKRGRPRT"
     gene            2299..2715
                     /locus_tag="QGF01_02065"
     CDS             2299..2715
                     /locus_tag="QGF01_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HJN19717.1"
                     /translation="MNKKIGIVIGVIVLGMIFVVSNINFEEQAEITQPEEPEEPENLV
                     LMPVKAARPDCGPNDECYIPAKITIKPGETVYWKNQDAAFHSVTSGFYDDPDGLFDSE
                     LLDPEDIFSYKFTEEGIYDYYCTLHPWMKGIILVER"
     gene            complement(2710..2783)
                     /locus_tag="QGF01_02070"
     tRNA            complement(2710..2783)
                     /locus_tag="QGF01_02070"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2747..2749),aa:Cys,seq:gca)
     gene            complement(2824..3045)
                     /locus_tag="QGF01_02075"
     CDS             complement(2824..3045)
                     /locus_tag="QGF01_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HJN19718.1"
                     /translation="MKSSELGLSPMYRILKKSGAERVSDESANELRRVIEEIAESIAK
                     NAVDMSTHAGRKTIKAEDIKLASKQFNKY"
     gene            3147..3902
                     /locus_tag="QGF01_02080"
     CDS             3147..3902
                     /locus_tag="QGF01_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HJN19719.1"
                     /translation="MNWIYDTHIHLSDLSYESYLPFILNSMKKIHLKACCVSMDYTSA
                     KTTLELGKKSDLIFPFIGIHPEKANGSTESILTLIEENKEQISGIGEIGLDDTYSDSD
                     EELSRQEEIFRTQLSLAEKLKKPVSIHSRKTLDKIFKILPSYNVTNVLLHWFDGNKKQ
                     LRDAMDLGCFVSFGPVMVYSQDKQVLLTKADKDKILVETDGPVRFSRCFGYKEAQVSF
                     IPSVIFSASKVLGVTYNELSEILEQNSKSYLDI"
     gene            3934..4305
                     /locus_tag="QGF01_02085"
     CDS             3934..4305
                     /locus_tag="QGF01_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19720.1"
                     /translation="MNRIKRLSTMVLDDYKDRFGDSFDDNKKILNDVSTIRSKSLKNK
                     IAGYITKLIKNEIRNNAEKENQQSNQKTTVDTEVPVDTEVPVDTEVPVDTEVPVDTEV
                     PVDTDKTEPTESPTKDTDTNE"
     gene            4321..>4675
                     /locus_tag="QGF01_02090"
     CDS             4321..>4675
                     /locus_tag="QGF01_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HJN19721.1"
                     /translation="MITIKFMGGAKKSFSTDKILLEKTNLTITELFEHLIQIKSSDTL
                     EFDTNNLLVAINGVDSSVLDGYNTSLKDNDVVSIIPIIHGGKPDRIQFVLLGSNVEMF
                     YIKYNKKFDSQFLDQI"
BASE COUNT         1520 a          782 c          757 g         1616 t
ORIGIN      
        1 acgaataatt tatttttctg aaggctcaac ccttacatgt tcaccaggat aaaactccgc
       61 aagttttcgt gaataacatc taccacacaa aaaaccatcc attttccatt cttccattgg
      121 gacataacta agttccatct ctgtattaca tagtgaacat ttacgatgcc ctttcatcaa
      181 tcacattttt gtactgatta taaaaactat tttaaatctc tattgatttt ttatacaaaa
      241 tccatatttg ttatatagaa gtttttttta tagattaacg gtagaatatc tggtcattgt
      301 aaacctcctg caagaattta cttggctgga tgtctacctt tatttagaat tacttgatta
      361 aagaccagaa attgtttttt tgatattgct tatttcttca gattctaaac tgatgtaatg
      421 ttcaattact ttcttcattt cataattgta aatacttttt gagaataggt tatgaggtga
      481 attgtttact ttttctaatt cttctcctaa atcacgacta gctagtactt ggtctacaat
      541 tttatctgtt agtctcagaa ttcttttgaa taatgaaaca tttctaatca tactttctag
      601 tttagaaata tcattatcaa aatcatcttt tgtctctaaa atatactcat gaataatttt
      661 tccagttact cctacaaaaa atattgttga atatcgcttg aatgggtgtg ttttattctt
      721 tggaccgtat tcaagttcat ttttagcaca ttccatgatt agagttggga acaagaaaat
      781 cctgtagtca tccataagtt cttcagtaaa gattttttca tattctgctc cacctggaag
      841 aaatttggaa attgattggt atgcaccaga aggattctgt ttgaaatatg caacataagt
      901 ttgaatggca tcttttgagg ctaatttctt tttatgattt tctgggaggt attgattaaa
      961 aatttcatta ccttggaatt ttgctctctg accagcatca agtctatagt cccaagaacc
     1021 tgattgatat tcaaaaaagt atcctgttcg tgtatctatc tgataactaa tagatttcca
     1081 gtaatcctct agcgctacaa gatccttacc ttttactgca ttttgggaat tgctaaatct
     1141 tgtgatactt ttttgttgtt cttcatcatc cgttttaatt acttttaatt ggatttcagc
     1201 gtctaggttt tttgttttct tggacacatc aagtattccg tgtgatgttt gagcaccatt
     1261 aacaattatt ggagaatcac attcaatctt tccttctaga tcattaaagt caacacaggt
     1321 catcgttagt ccgttattgt attcggcaaa tctttcagga cattcctcta atgtctttct
     1381 aattccccta tttactttca ttctatgacc taaatgatgt cgaatattgt tatcaagaat
     1441 gtacttgttt ctatctacta aatttgctat ttctattccc tttactgtac aataaaaaat
     1501 attatttctt tggtaaccat caagataatt tatagtagct ttatgtcctt tttcaggttt
     1561 catttttcgt tcagataaaa tttgaacagt ttgtgccaaa tctataacac gaacaacatc
     1621 acattcgtat tcagcatatt gttcagtgac atatactagg tttattttta catttttttg
     1681 attgatttca ttccaaagat atgatagatc gtcacgtttg atccttgact gttctgcgtt
     1741 tttaaatcgc tcaacgtctt taacaaattt atcaatcata ccgatgttgt gagattctcc
     1801 aaattttgac tggacaagag taatttcttt tttatcttca tatcgtaata cggcatctat
     1861 tccacagtca tctggaccat caataattgc attctctgca ttttcaactg tttcctcgaa
     1921 aactttagtc agcatccaga taagataatc atggcctttc tgaacagtat tggttgcttc
     1981 actggtagct tcttttattg aatcttctac agacctagaa aaaccttcaa gtggtttttt
     2041 ttctgagaga ataagacgcc cttcttcact aaaaaggcgg atgtccgcta ctctagaggg
     2101 caaattcatc attcgtcttt ccttctcctt atcaagtagt gggtaattat gagataaccc
     2161 catcactaag aattctttga ttagagaagc tgttgaagtt tggatgaaag aaatggatgt
     2221 aacatatcat gaaactctaa cagaaaagaa gaccagagga agaccaagaa ttaaaaggaa
     2281 acgtggaaga ccaagaacgt gaataaaaaa atcggtatag taattggagt aattgttttg
     2341 ggcatgatat ttgttgtctc aaacataaat tttgaagagc aagctgaaat cacccaacca
     2401 gaagaacctg aagaacctga aaatctagta ctaatgccgg taaaggctgc tcgtcctgat
     2461 tgtggtccaa atgatgaatg ttacattcca gcaaagatta cgataaagcc tggcgaaaca
     2521 gtttattgga aaaaccaaga cgctgcattt catagtgtaa caagtggttt ttatgatgat
     2581 ccagatggat tgtttgacag cgaattatta gatcctgaag atatattttc ttacaagttt
     2641 accgaggagg gaatttatga ttattattgt acgcttcatc cctggatgaa aggcattatt
     2701 ttagtagaaa ggtgagggag agatttgaac tcccgacact aggctttgca ggcccatgca
     2761 ctaccagact gtactacctc accgtacaga aaaaaataac aaatgagcaa ttaactcttt
     2821 agattaatat ttgttaaatt gcttcgaggc taattttata tcctctgcct taatcgtttt
     2881 ccttcctgca tgtgttgaca tatctactgc atttttggca atagattctg ctatttcctc
     2941 tataactctt ctaagctcat ttgcagactc gtcactaaca cgctctgccc cagatttttt
     3001 tagaattctg tacatggggg aaagtcccaa ctctgacgat ttcatgatta tttttgttta
     3061 aagtatgtta aaaaaccact agtaaaataa ttcagataga ataccgattt aaacagactt
     3121 taactcatga ccaatctagt ataatcatga attggattta tgatacccat atccatcttt
     3181 cagatttatc atatgaatca tatcttccat ttattctaaa ttctatgaaa aaaattcatc
     3241 ttaaagcatg ctgtgtttcg atggactaca cttccgcaaa aaccacttta gaacttggaa
     3301 aaaaaagtga tctaattttt cctttcatag gcatacatcc tgaaaaagca aatggcagca
     3361 ctgaatcaat attaacactc attgaagaaa ataaagaaca gatttctggc ataggcgaaa
     3421 taggattgga tgatacatat tctgattccg atgaagagtt gtcaaggcag gaagaaatct
     3481 ttagaacaca gctttcattg gctgaaaaat tgaaaaaacc tgtttccata cattctagga
     3541 aaacacttga taagattttc aagattttac catcatacaa cgttaccaat gttctgttac
     3601 attggtttga tggaaataag aaacagttgc gtgatgcaat ggatttgggg tgttttgtat
     3661 cgtttggacc agtaatggtt tattcacagg ataagcaggt attgctaacc aaagccgaca
     3721 aagataaaat tttagtggaa accgatggtc cagtaagatt ttctagatgt tttggttaca
     3781 aagaggcaca agttagtttt atcccaagcg taattttttc tgcatcaaaa gtattgggtg
     3841 tcacatacaa tgagttatct gagatattag aacaaaattc caaatcttat cttgatatat
     3901 agatataaaa aacccgccaa attcttgagg ttagtgaata gaataaagcg actttcaacg
     3961 atggttttag atgattataa ggatcggttt ggagatagtt ttgatgacaa taaaaaaatc
     4021 cttaacgatg tatccacaat tcgttctaaa tctctcaaaa acaagatagc cggatatatt
     4081 acaaaattaa ttaagaatga aataaggaat aacgctgaaa aggaaaatca acagtcaaat
     4141 caaaagacca ctgttgatac ggaagtacct gttgatacgg aagtacctgt tgatacggaa
     4201 gtacctgttg atacggaagt acctgttgat acggaagtac ctgttgatac ggataaaaca
     4261 gaacctactg aatcaccaac aaaagatact gatactaacg agtagttttc aattaaatta
     4321 ttgattacta taaaattcat gggtggtgcg aagaaatcat tttccactga taaaatatta
     4381 ctagaaaaaa ctaaccttac aataacagag ttatttgaac atcttattca gattaaatct
     4441 agtgatacat tggaatttga tacaaataat ttacttgtgg caatcaacgg agttgattca
     4501 tctgtgttag atggttataa tacgagttta aaggataatg atgttgtaag catcatacca
     4561 attatccatg gagggaaacc tgatagaata caatttgtat tattaggttc aaatgtggag
     4621 atgttttata taaaatataa taaaaaattt gactctcagt ttttagatca aataa
//
LOCUS       DASCPJ010000043        15701 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000759982, whole genome shotgun sequence.
ACCESSION   DASCPJ010000043 DASCPJ010000000
VERSION     DASCPJ010000043.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15701)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000759982"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="QGF01_02095"
     CDS             complement(<1..583)
                     /locus_tag="QGF01_02095"
                     /EC_number="3.5.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase IIa"
                     /protein_id="HJN19722.1"
                     /translation="MIQLTIMKITGYGPWTLTLGSDREHELQMLQASLYQQVQNLFSQ
                     KNGLVFSNRSDEFFAVTNGITLEDHIEIQKKLESSFDLKLSMSIGYGYSPFDANIRAY
                     DGKKSGKMLNEKHNIFGFIDGKEESKVTIMHLDIDDLASTRKTISPYEISSLMFKLYA
                     KMSEHFLAKKSLSFFMGGDNFMVVADSDYKKNAK"
     gene            complement(585..998)
                     /gene="ribH"
                     /locus_tag="QGF01_02100"
     CDS             complement(585..998)
                     /gene="ribH"
                     /locus_tag="QGF01_02100"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HJN19723.1"
                     /translation="MNIAIVVSEFNNKVTSRMHQVALEKSKELKLKVKYDCKVPGIFD
                     MPLILDILLQKKDVDGIVTLGAVIKGQTKHDEVITNAVAARISKLSIKYQKPISLGVS
                     GPGMSERQAFARIRPVSERAVQAISSIAEELKRIE"
     gene            complement(1001..1675)
                     /gene="ribB"
                     /locus_tag="QGF01_02105"
     CDS             complement(1001..1675)
                     /gene="ribB"
                     /locus_tag="QGF01_02105"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HJN19724.1"
                     /translation="MTFDTAISSLKEGEFVMIYDSDGRESEVDLCVAAQFITPEHVAR
                     MRQHAGGLLCLAISNGLAKKLDLEYMHNILANSNDLDIDSKNMIMGTAPYGDHPTFSI
                     SINHKRTYTGITDSDRALTINEMANLYNTDNPKKQFTSSFKTPGHVPLLLASDGLLSS
                     RKGHTEMSIYLTKLAKLHPVSAICEMMDAETYAALSVEKAKKYAKENAIPFIDGKKLY
                     EFSKVR"
     gene            1782..2366
                     /locus_tag="QGF01_02110"
     CDS             1782..2366
                     /locus_tag="QGF01_02110"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HJN19725.1"
                     /translation="MFTGIIVGEGKVTKLNPKPKSRSAIQMTVDLGKYAKGLKIGQSV
                     AINGVCLSATKISGKKCIFEMIDETTKKTDLGNVKAGSTVNIERSLKIGDRLEGHFVL
                     GHIDGVATLTKIEKKPKEVKFWFKVPKKLVKYVVKKGSIALDGISLTVVDVKKDLASI
                     CLIPHTIKVTNFKSKKIGDKLNIETDILGKYIKK"
     gene            complement(2478..3329)
                     /locus_tag="QGF01_02115"
     CDS             complement(2478..3329)
                     /locus_tag="QGF01_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HJN19726.1"
                     /translation="MPNLFRSLVFVPGNNPRFLEKAKSLPADVVCFDLEDSVPDKEKK
                     KARVLIKNTLKKRNQYSASIFARTNSPDSGKIAADLKEIVQKGIDGIVIPKVNSAKEL
                     KKIEKIISSLEKKRKIKGIRLIPSIESVLGVVNCYEIASASKRIDALVFGIFDLLNDM
                     GIEYTKGNPRGAKYSRYKVPVAAAAAGVAAIDGIWQDLKDKKGFTQDCKVGKSLGYAG
                     KSVIHPDQIKTVHKIFHPSKPEILWAKKVCSVYEKSTKKGKGATVVDGKMIDEVHYKW
                     AKAVLEI"
     gene            complement(3337..4416)
                     /locus_tag="QGF01_02120"
     CDS             complement(3337..4416)
                     /locus_tag="QGF01_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJN19727.1"
                     /translation="MKGLVYEEYAPDDDFKKILKVKDVDDPKPKPDEVVFKVKAAALN
                     FNDIWGMRGQPVPVPLPHISGSDAAGEVIAVGDEVTKIKVGDRVMSHSNMSCRVCSAC
                     TDGREFDCEKRTIWGFQTGPNWGAYSEITHLPEVNVVKLPDNVSFDEAAAASMTLLTS
                     WHMLVGRAKIKPGQTVLIMGGGSGVGSFGIQIAKLYNCKVIATASPDKLDKCKQLGAD
                     YAVDHRKDDWHKEVRAITKEIAKETGVSPGIDISFDHIGQTHWNKQLQNLKYGATLVS
                     CGATTGYDAMTDLRHVFFKGINILGSTQGTRAELEEGVYWMGQGKIKATIDSTWSFEQ
                     AHEAHTKMLTGKGLFGKILMKPEGA"
     gene            complement(4458..4745)
                     /locus_tag="QGF01_02125"
     CDS             complement(4458..4745)
                     /locus_tag="QGF01_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HJN19728.1"
                     /translation="MSYPNPDNENNIPQEKDSELKAQKEMMLKQALSSDARLRLNNVR
                     MVKPDLAEMVENYLLNLIMQGKISGQISDEQLKQILLSTQKPKRDFKINRI"
     gene            complement(4753..5205)
                     /locus_tag="QGF01_02130"
     CDS             complement(4753..5205)
                     /locus_tag="QGF01_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HJN19729.1"
                     /translation="MAKVYDVPADVFISRLAEILKTEDIPAPNWTVFVKTGAHADKPP
                     QNPEWWHTRCASILRKIYLQGPITVNELRSIYGGSKPHGYGAARHRYASGAIIRNAIH
                     GLEKLGYVEKIEKKGRVITKQSMQKLDRLATEILSEFIVKEPRLKIYS"
     gene            complement(5237..5539)
                     /locus_tag="QGF01_02135"
     CDS             complement(5237..5539)
                     /locus_tag="QGF01_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit"
                     /protein_id="HJN19730.1"
                     /translation="MNPSRKKIALERMHILVKIAISNARSDPNLAEKQANLARRISTH
                     YRIRMPYELRINFCKKCKKFIVPGINSRIRIGRTHLKSIRITCNFCEHTYRKVIAQ"
     gene            complement(5586..5659)
                     /locus_tag="QGF01_02140"
     tRNA            complement(5586..5659)
                     /locus_tag="QGF01_02140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5622..5624),aa:Gly,seq:tcc)
     gene            complement(5680..6354)
                     /locus_tag="QGF01_02145"
     CDS             complement(5680..6354)
                     /locus_tag="QGF01_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis protein"
                     /protein_id="HJN19731.1"
                     /translation="MKRLSFILAESSLETVPKKIQNHPSVISHSKKLGKAPSEILLDN
                     SWHYAAMKGIENENKRGRPDIVHSSLLEACSIPLYFEKKISIYIHTINDEIIKINDNV
                     RLPKSYHRFVGLIEQLFSEKRIESEGNILLEIVNMKFSELIKKINPKSIVGLSRLGEM
                     SSYQNVAQTLDDDSCIIIGGFQKGHFSEEVKNTIEQVFSINQKPLESHIVTARILYEY
                     EKTIFM"
     gene            complement(6382..9039)
                     /locus_tag="QGF01_02150"
     CDS             complement(6382..9039)
                     /locus_tag="QGF01_02150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HJN19732.1"
                     /translation="MEETKSQIENSIAQVRKRDGSISNFNRDKILTAIHKAFSATNNP
                     DKEIATKLSDGVLEKLVEHGFSATNPPSVESIQDLVESTLIDQGHSDIAKGYILYRHE
                     RRKIREAKMKLLNTKNLDAVSKSFDINCLRVLASRYLLRDHKNEINENPSELFERVAV
                     LVAISDMLRDNELFTIEGNVSQNTDEAIEYLKKLDDFNYKFKIGTYYLNKWHFRCLIN
                     CYADLANQGKMKVSFKELLTMLASKKLDRYADKISEYYELMVSQDFLPNSPTMMNAGG
                     RLGQLSACFVLGMPDDMAGIMKSTSDAALIFKSGGGVGINYSDLREEGEIVASTSGVA
                     SGPVSFMNIINSVTEVVKQGGKRRGANMGIMEVWHPDIEKFITNKTEPGVLENFNVSV
                     GVWKDFWDTLVNSDDGKYTLRCPRDNSPAREINAHHLIDLIAQSAWKSAEPGLIFFDN
                     INKNNVFAKARGQLLRATNPCGEQSLYPYESCNLGSINLANLVKRTADGQYEFDWQRY
                     EETVRKTTRYLDNIIDVNLYPIPEIDKASKESRRIGLGVMGVADLLYKLRIPYNSKEG
                     YDFQLKLAEALTYYSMEESVALAKSRGKFVLCSSTEYPEGKIPVSGYYEKPKEEQSYD
                     WDALITKIQKYGIRNVLTTTVAPTGTLSMLADCSNGMEPNFALVFEKRVTVGRFFYTN
                     KIFKEVLKENNLYSDELLAKVADNYGSVKGIPEIPDWIQNIFVTAMDIHWSDHVMAQA
                     VWQDWISNAIAKTINMPNDVSVEDVKAAYLLSHELGLKGITVYRDGSRHKQVLHMTGE
                     NSEKKFDVTPSDFVKNYISTNIKNAYVKSQIDNALSAVKLEEIQRPMQSVQEDEKEET
                     LCPVCKNKLIFAEGCNICIDCGYSGCTSG"
     gene            complement(9205..11379)
                     /gene="nrdD"
                     /locus_tag="QGF01_02155"
     CDS             complement(9205..11379)
                     /gene="nrdD"
                     /locus_tag="QGF01_02155"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HJN19733.1"
                     /translation="MLGSKESIALEDELDLNADLDLDDELDGAGLDADDLDEPSETGA
                     LQSSSKRVRMIFSVMASPNRIDILRILNSKGPLTYSELKSLAGFKSKKESGKFAYHLR
                     KLLRQSLVALNKSERRYTITNLGKLVLSLARQIEERSIIESGKMYVRTSHDSIEEFNS
                     HKIIQSLVREGNLPLELAQKITEEVENRIYKYQTAYLTGSLIRELVNSVLLEHGYEEY
                     RHKLARVGLPAFEVQETISNAENLDSGIESLLFNTGQTVFAEYLLTNTLPKDIADEHL
                     SGDLHITRPGLWSLLPDSIFINIKELIEDGIDLKGKALSVSKLTSIKTLSNLSSALSM
                     IISLIAKEASQEVIMDGLVLLLSKYSKNLSELEEKLVNSFIMSSTAFKFNKTPTIVSF
                     TLPLGVEQKIVNVVLSAYRTYVELTPRPRIGLVINYEKGKITDVSNILSEIISLGGEI
                     IFAKHNISQNGVTRLKSTSSSTLHLGSLSINLPRLAFESNKDETYFRARLALLMKPAL
                     VSMNLRKKNISELVRLGMNPILANNTQYMQRCSTSLVINLVGLREAVFGILGFKDDKQ
                     GHEILHKVIETSVNIALKKAKDLGTDVLVTMTKSDGSGRFISLDEEKYGKNSILKITE
                     NETYTEGVVIDATTLADLTIKSPQIVECNRMAKTLNGGLFTQVKIPNGSKITEIKKNI
                     EKISNMTNSFKLIMRVPICGNCGLKDQTLVDKCPTCKSSYII"
     gene            complement(11432..11593)
                     /locus_tag="QGF01_02160"
     CDS             complement(11432..11593)
                     /locus_tag="QGF01_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19734.1"
                     /translation="MSVDIKCGKCGENISTMQMLKPIKDVLKHYNNKCPSCGQKLSTS
                     EFSLDIEKK"
     gene            complement(11663..12037)
                     /locus_tag="QGF01_02165"
     CDS             complement(11663..12037)
                     /locus_tag="QGF01_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HJN19735.1"
                     /translation="MCKLTIKDEFIANAKQGKILARKCKNCNDLNLVTVYFCKKCGKK
                     EFENTILDGNGVIVTYTIMTVPPSGFETFSPYAWVVMKIEGHDLNISGFLANIGKPAD
                     LPIGTAVKIIDFDKRGLVLEKR"
     gene            complement(12030..13184)
                     /locus_tag="QGF01_02170"
     CDS             complement(12030..13184)
                     /locus_tag="QGF01_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HJN19736.1"
                     /translation="MEKVCVLGAGSTKYGKLEDSITDITLQASVSAIESAGIESKEIK
                     SGYISNVFGVADKQVHLGPVIMSNLGISDKPSLSIESACGSGSVSFREAYANVAAGFY
                     DVVLVTGVEKVTHTGTEWTTTYFSYCSDFFYEGQAGVSFPGLFASMARAYITEFNASE
                     EDFAAVAVKNHENGLLNPKAHLRKKITIDDVMNSAVVASPLKLYDCCPFSDGASAVII
                     CSEKFAKQQGGDYVEVIGSGRGGSPAALQGRDHITTIPSTKIAADAAYKMANITSKDI
                     DFAEVHDCFTIAEVVDTEDLGFFEKGKGIDAIRNGVTKLNGQLPINPSGGLKSKGHPI
                     GATGVGQVVEVYDQLTGNAGERTVNDAKIGLTQNFGATGASCAVHIFQSV"
     gene            13256..13717
                     /locus_tag="QGF01_02175"
     CDS             13256..13717
                     /locus_tag="QGF01_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HJN19737.1"
                     /translation="MKKRIQSLPLTKTKKSISVTKPTKKKIKILKNELEQYLDSNGYI
                     SYSTKKKKYVILGTNSPKNGIVGCPQCGIGQLMIIKSPITKKRFIGCSNYNNGCTASS
                     PLLQKATLRVIKAKCQLCKWPIVVFRYARNQNWKRQCSNIECKSRKTKISV"
     gene            complement(13691..14503)
                     /locus_tag="QGF01_02180"
     CDS             complement(13691..14503)
                     /locus_tag="QGF01_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HJN19738.1"
                     /translation="MSKVAIVQFKASTDKTKNLPKILDYIKQAAKNHADLCAFPEYMM
                     FYTPRTQSAKQVARQAETINGKFVSTIRECARKNSIIVIGTMYEKSRKKDRVFDTSFI
                     INKNGKISAKYRKIHLFDALGFKESSKLSAGKTLPPTTKTSVGKLGMMICYDLRFPEL
                     SRILASSGSEILVVPSAWVKGPMKEEHWFTLNKSRAVENGCYVIAPNHLGNIYSARSL
                     VVDPYGKIILDMKKRQGLGYVNISSAVIQKIRKKLPLLKNRRTDLYTDFGFS"
     gene            complement(14538..14714)
                     /locus_tag="QGF01_02185"
     CDS             complement(14538..14714)
                     /locus_tag="QGF01_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HJN19739.1"
                     /translation="MPLITVSMYPGRTQQQKNDFAKSITKSAVEVLKTKEEHVIVVFD
                     ENPKENWFVAGSQL"
     gene            complement(14750..15520)
                     /locus_tag="QGF01_02190"
     CDS             complement(14750..15520)
                     /locus_tag="QGF01_02190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HJN19740.1"
                     /translation="MLPIGDENPHPPGFKPKLTYALIAINVIIFLFEVAVTGQFFEFS
                     NRQAMNMFLNWGAVPGCITGQISGIDTGINIINCPAIPELTLLSSVFMHGGLMHLGGN
                     MLFLWIFGDNIEAKFGRVKYLGIYLLWGIGAGLIHVMGDTSTGIPAVGASGAISGVLG
                     AYLVMFPHAKVKTFLMLGFFWRMLHIKAMYFLPFWLIFQNLLPFFIGGFGVAGGGVAY
                     LAHIGGFVLGLAVGYIYKKSRGSASMYGTRYGYRGDYR"
BASE COUNT         4869 a         2918 c         2361 g         5553 t
ORIGIN      
        1 ccttagcatt ttttttgtag tccgaatctg caacaaccat aaaattatca ccgcccataa
       61 aaaatgatag tgactttttt gccagaaaat gctcagacat ttttgcatat aacttaaaca
      121 tcaatgaaga aatttcatat ggtgatattg tttttcgtgt agaagctaaa tcatcaatat
      181 ccaaatgcat gattgtgact ttcgattctt cttttccgtc gataaatcca aaaatattgt
      241 gtttctcgtt cagcattttc ccagattttt ttccatcata tgctctgata tttgcatcaa
      301 atggtgaata gccatatcct attgacattg atagtttcaa atcaaatgat gattcaagtt
      361 tcttctgaat ctctatatga tcttctaacg ttatgccatt tgtcacagca aaaaattcgt
      421 ctgagcgatt tgaaaaaaca agaccatttt tctgagagaa taaattttga acttgctgat
      481 aaagtgatgc ttgaagcatt tgcaattcat gctctctatc acttcctaat gtcaaagtcc
      541 atggaccata tcccgtaatt ttcattatcg tgagttggat caatctattc tatcctcttt
      601 agctcttctg ctatactaga aattgcttga acagcccttt cagatactgg acgaattctt
      661 gcaaaagcct gcctttcaga cataccagga cctgaaactc ctaatgagat tggtttttga
      721 tatttaattg ataatttaga aattcttgct gcaactgcat ttgtaataac ttcatcatgt
      781 tttgtttgtc ctttaattac agcacctaat gttacaatcc catcaacatc ttttttttgt
      841 aataaaatat ccaagatcaa tggcatatca aaaataccag gtactttaca gtcatacttt
      901 acttttaatt ttagttcttt tgatttttct agagcaactt gatgcattct agatgtcact
      961 ttattgttaa attctgaaac aactattgca atattcaaaa ttatctcacc tttgagaatt
     1021 catagagttt tttcccatca ataaatggaa tggcattctc ttttgcatat ttttttgcct
     1081 tttcaactga aagtgcagca taagtttctg catccatcat ttcacatata gcagaaactg
     1141 gatgtaattt tgctagctta gttaggtaaa ttgacatctc tgtatgacct tttctagaag
     1201 aaaggagacc atctgatgca agtagtaatg gaacatggcc cggagtttta aaggaggaag
     1261 tgaattgttt ttttggatta tctgtattat acaagtttgc catctcattg atagttaaag
     1321 ctctatcact gtcagtaatt ccagtgtatg tacgcttgtg gtttatagat attgaaaaag
     1381 ttggatgatc accatatggt gctgttccca ttatcatatt ttttgaatct atatcaagat
     1441 catttgaatt tgcaagtata ttgtgcatat attctagatc taattttttt gctaaaccat
     1501 ttgaaattgc aagacaaagc aaaccacctg catgttgtct cattctagca acatgttcag
     1561 gagtgatgaa ctgagctgca acgcagagat caacttcgct ttcacgtcca tctgaatcat
     1621 aaatcataac aaattcgcct tcttttagag aagatattgc agtatcaaat gtcattattg
     1681 aaaattgatt tttggatctt ataaacttac taaaaatatg gtaattacca cttttttggt
     1741 aataagtgta gttcatatat tatcgctaaa ttactaaaat aatgttcact ggcataattg
     1801 ttggagaagg aaaagtcaca aaacttaatc caaaaccaaa aagtagaagc gcaattcaaa
     1861 tgacagtcga tcttggtaaa tatgcaaagg gattaaagat tggacagagt gttgctataa
     1921 acggagtttg tttgagtgca acaaaaattt caggtaaaaa atgtatcttt gagatgattg
     1981 atgaaacaac aaagaaaact gatttaggaa atgtaaaagc aggaagtact gttaatatcg
     2041 aacgcagttt aaaaattggt gacagattag agggtcattt tgttttagga catatagatg
     2101 gagtagcaac actgacaaaa attgaaaaaa aaccaaaaga agtaaagttt tggttcaaag
     2161 ttccaaaaaa attagtaaaa tatgttgtaa aaaaaggttc gattgcactt gatggaatta
     2221 gtttgacagt ggttgatgta aaaaaagatc ttgcatcaat ctgtttgata ccacatacaa
     2281 tcaaggttac taattttaaa tcaaaaaaaa ttggagataa actcaacatt gaaacagata
     2341 ttctaggaaa atatattaaa aaataataat tattcttcgc tttcgtgaag gtgtttcatt
     2401 cgttcggatt gcgtggaaca gctagtttga agaataaata tcgagaatgg tcaacaaaag
     2461 ccaaaactaa atgaaaatca aatttctaaa actgcttttg cccacttgta gtgaacttca
     2521 tcaatcatct ttccgtcaac aacagtagca ccttttcctt tctttgttga tttttcataa
     2581 acactacaaa ctttttttgc ccataaaatc tcaggtttac taggatgaaa aatcttatga
     2641 acagttttga tttgatctgg atggataaca ctttttccag catagcctaa actttttcca
     2701 actttacaat cctgagtaaa acctttttta tctttcaaat cttgccatat accatcaatt
     2761 gcagctacac ctgctgcagc cgctgccact ggaaccttat atctggaata ttttgctcct
     2821 ctagggtttc ctttggtata ttctatgccc atatcattca acaaatcaaa tataccaaaa
     2881 actagagcat caatacgttt gcttgctgag gctatttcat agcaatttac aacacctaga
     2941 acagattcta ttgagggtat caaacgaatt cccttgattt tccttttttt ctctagagat
     3001 gagattattt tttcaatttt tttgagttct tttgctgaat ttacctttgg aatcacaatt
     3061 ccatcaatcc ccttttgtac tatctctttc agatctgctg caatcttgcc agagtctggt
     3121 gaatttgttc ttgcaaaaat ggatgctgaa tattgatttc ttttctttaa tgtattcttg
     3181 atcagtactc tagctttttt cttctctttg tctgggactg aatcctcaag atcaaaacaa
     3241 acaacatcag ccggaagcga ttttgctttc tctaaaaatc ttggattatt tcctggaaca
     3301 aaaactagac tacgaaatag gtttggcatt aactgattaa gcgccttcag gcttcattag
     3361 gattttgcca aacaaacctt taccagttag catttttgta tgagcttcat gagcctgttc
     3421 aaatgaccaa gtagaatcaa ttgtagcctt tatttttcct tgacccatcc agtatacacc
     3481 ctcttctagt tcagcacgtg ttccttgtgt tgaaccaagt atgttaattc ctttgaaaaa
     3541 tacatgacga aggtcagtca ttgcatcata tccagttgtt gcaccacaag aaaccaaagt
     3601 agcaccatat ttgagattct gaagttgttt attccaatgt gtttgaccta tatgatcaaa
     3661 agatatatca attccaggag aaacacctgt ctcttttgca atctcttttg ttattgctct
     3721 aacttcttta tgccaatcat cttttctatg atctacagca taatcagctc ctagttgttt
     3781 acatttatcc agtttgtcag gacttgctgt ggcaattacc ttacaattgt ataattttgc
     3841 aatctgaatt ccaaaacttc ctactccaga accacctccc atgataagta cagtttgtcc
     3901 aggtttaatc ttggctcttc caactaacat atgccatgat gttaaaagag tcattgatgc
     3961 agcggctgct tcatcaaaac ttacattgtc aggaagttta acaacattaa cttctggaag
     4021 atgagtgatt tctgaatatg cgccccaatt tgggccagtc tgaaaacccc agattgttct
     4081 tttttcacaa tcaaattctc tgccatccgt acatgcacta catacacgac atgacatatt
     4141 cgaatgtgac attactctgt caccgacttt gatcttagtt acttcatcac caacagcaat
     4201 aacttcacct gctgcatcgg aaccagaaat atgaggtaat ggaacaggta ctggttgacc
     4261 tcgcattccc caaatatcat taaagtttag tgctgcggct tttaccttaa aaaccacttc
     4321 gtccggtttt ggttttggat catcaacatc ttttacttta agaatttttt taaaatcatc
     4381 atcaggtgcg tattcttcat acactaagcc tttcatgaaa aattttgaat ttttccccta
     4441 attatatttt cttgctgtta aattctgttg atcttgaaat cacgcttggg tttctgggtt
     4501 gataacaata tttgtttcag ctgttcatca gatatttgac cagaaatttt tccctgcatt
     4561 attagattca aaagatagtt ttcaaccatt tcagccaagt caggttttac cattctaaca
     4621 ttatttaatc tgagtctagc atcagatgat aaagcctgtt ttaacatcat ttccttttgt
     4681 gcttttagtt cggaatcttt ttcttgagga atattgttct cgttatccgg attaggatag
     4741 ctcataacat cactaagaat aaattttcaa acgtggttct tttacaataa attctgataa
     4801 tatttcagtg gctaatctat ctagtttctg cattgattgt tttgttatca cacgaccctt
     4861 tttttcaatt ttttctacgt atcctaattt ttcaagtcca tgaattgcat ttcgaataat
     4921 tgccccactt gcatatctgt gacgagcagc accatatcca tgtggtttac tacctccata
     4981 tattgaacgt aattcgttca cagtaattgg accttgtagg taaatttttc tcaagattga
     5041 tgcacaacgt gtatgccacc attctggatt ttgtggtggt ttatctgcgt gagcaccagt
     5101 tttaacaaat actgtccaat ttggagctgg tatatcttca gtttttaaaa tttcagcaag
     5161 tctgctgata aaaacatctg ctggtacatc ataaacttta gccatttaat gtaaaacttg
     5221 taaatcgtct tataaatcat tgagctatta ctttacgata ggtatgctca caaaaattac
     5281 aagttatccg aattgatttt agatgtgttc taccaatcct aattcgtgaa ttgattccag
     5341 gaactatgaa ttttttacat tttttacaaa aatttattct taattcatat ggcattctaa
     5401 ttctataatg tgtactaatt cttctagcca agttagcttg tttttcagct aaattaggat
     5461 ctgatctggc atttgaaatt gctatcttaa caagtatatg catacgttca agtgcaattt
     5521 tttttcttga tggattcata ttttttagat aatcatgtca ctaaaaattg gatcggatta
     5581 aaaaatgcgg tcagcgggat tcgaacccgc gtcacagggt tggaaacccc gaatactaac
     5641 caggctatac tatgaccgca taggttgcaa atttttttgc tacataaaaa tggttttttc
     5701 gtattcataa agaattctag cagtcacaat gtgtgattcc aatggttttt ggtttatact
     5761 aaatacctgt tcaatcgtat tttttacctc ctcagaaaag tggccttttt ggaatcctcc
     5821 tattataata catgaatcat catctagtgt ctgcgcaaca ttttgatatg aactcatttc
     5881 acccaaacga gataagccaa ctatactttt tgggttaatc tttttaatca attctgaaaa
     5941 tttcatgttc actatctcaa gtaagatgtt tccttccgat tctatccttt tttctgaaaa
     6001 taattgttca attaaaccca caaatctatg ataggatttt ggtagtcgaa cattatcatt
     6061 aatttttatt atttcatcat ttatagtgtg aatgtaaata gagatctttt tttcaaaata
     6121 tagtggtatg ctacaagcct ctagtaaact agaatgaaca atatctggcc tgcctctttt
     6181 attttcgttt tcaattcctt tcattgcagc gtagtgccaa gaattatcaa gtaatatttc
     6241 tgatggtgct ttaccaagtt ttttactgtg agatataaca gatggatgat tttgaatttt
     6301 ttttggtaca gtctccagtg atgactcagc tagtatgaat gatagtcttt tcattaaatt
     6361 acaagtttaa aaaattgtat gctaacctga tgtacatcca ctataaccac agtcaatgca
     6421 aatattacat ccttcggcaa aaataagctt gttcttacat acaggacaca aagtttcttc
     6481 tttttcatct tcctgaacgg attgcattgg tctttgtatc tcctctaact taacggctga
     6541 taatgcgtta tcaatttgag atttgacata tgcatttttg atgttggttg aaatatagtt
     6601 tttaacaaaa tcgcttggag ttacatcgaa ttttttttct gaattttcac ctgtcatatg
     6661 caagacttgt ttatgccttg aaccatctct atatactgtg atccctttca atcccaattc
     6721 atgtgataac agatacgcag ccttaacatc ctcaacagat acatcattag gcatattgat
     6781 tgtcttagca attgcatttg atatccaatc ttgccataca gcctgtgcca taacatgatc
     6841 agaccagtgt atgtccattg ctgtcacaaa gatgttttga atccagtcag gaatttcagg
     6901 aatgcctttg acagaaccat aattatctgc gactttggca agtagttcat cactgtacag
     6961 attattctct tttagtactt ctttgaaaat tttatttgta taaaagaatc tgcctactgt
     7021 aactcttttt tcaaaaacta atgcaaagtt tggttccatg ccgttagaac aatcagccaa
     7081 catagataaa gttccagtag gagccacagt tgtagttaaa acatttctaa ttccatattt
     7141 ttgaattttt gttataaggg catcccaatc ataggattgt tcttcttttg gtttttcata
     7201 atatcctgaa acaggtatct taccttctgg gtattcagta cttgaacaca atacaaactt
     7261 acccctactt tttgcaagtg ctacactttc ttccatagaa taatatgtta atgcttcagc
     7321 taatttcaat tgaaagtcgt atccttcttt tgaattatat ggaatgcgta atttgtataa
     7381 tagatctgct actcccataa cacctaatcc tatacgtcga gattcttttg atgccttatc
     7441 aatttctggt ataggatata gattcacatc tattatgtta tccaaatatc tcgtagtttt
     7501 tctaacagtt tcttcatatc tttgccagtc aaattcatac tgtccatcag cagtcctctt
     7561 tacaagattt gccaagttta ttgatcctaa gttacaagac tcgtatggat agagactttg
     7621 ttcaccacat ggattagttg cacgaagaag ttgaccacgt gcttttgcaa acacattatt
     7681 tttgtttata ttatcaaaga aaatcagacc tggttctgca ctcttccatg ctgactgtgc
     7741 tatcaaatca atcaagtgat gtgcatttat ctctcttgct ggtgagttat ctcttggaca
     7801 acgtagtgta tattttccat catcagaatt tacaagtgta tcccaaaagt ccttccaaac
     7861 accaacacta acattaaagt tttctaagac cccaggttct gttttatttg taataaattt
     7921 ctcaatatct ggatgccaaa cttccatgat tcccatattt gcacctcttc tctttcctcc
     7981 ctgttttact acttcagtta cactattgat aatattcata aaagaaactg ggccagatgc
     8041 gacaccagat gtagatgcta caatctctcc ctcttcacga agatcagaat aattgattcc
     8101 tacgcctcca ccagacttga atatcaaggc agcatcagaa gttgatttca ttatgccagc
     8161 catatcatca ggcatgccca aaacgaaaca ggcagacaac tgacccagtc taccacctgc
     8221 attcatcata gttggtgaat ttggcaaaaa gtcctgggat accattaatt cataatattc
     8281 tgatatcttg tcagcatatc tatctagttt tttggaagcc agcattgtta atagttcctt
     8341 aaatgaaacc ttcatttttc cttggtttgc tagatcagca taacagttta tcagacatct
     8401 aaaatgccat ttattgagat agtacgtacc aatcttgaat ttatagttaa aatcatctag
     8461 ttttttcaga tattctattg cttcatctgt attttgtgat acgttacctt ctatagtaaa
     8521 caattcattg tctcttagca tatcagaaat cgcaaccaaa actgcaactc tttcaaataa
     8581 ttctgatggg ttttcgttaa tttcattttt gtgatcacgt aaaagataac gagatgctaa
     8641 cactctcaag caattaatat caaatgattt tgatacagca tcaagatttt tagtatttaa
     8701 aagcttcatc tttgcttctc gaatttttct gcgttcatga cgatatagaa tataaccttt
     8761 ggcgatatca ctatgacctt ggtcgattag tgtggattca accaagtctt gaatagattc
     8821 tacactagga gggttagttg ctgaaaatcc atgctctact agtttttcta aaacaccatc
     8881 agaaagtttg gttgcaattt ctttatctgg attattggtt gctgaaaatg ctttatgtat
     8941 agcagttagt attttatctc tgttaaagtt tgaaattgat ccgtctctct tacgaacttg
     9001 agcgatagaa ttctctattt gcgattttgt ttcctccaca agtctcccct ataagaccaa
     9061 taattgatat agcatataac ttctgtggca aaaaagaatt atctaaaatg aggtataagc
     9121 ttaactttgc taacaacgat ctaaaatttc ataataatat ttctagcgat ctattgaaat
     9181 gatagaatag aatgaattgt ggatctagat aatatatgat gatttacaag ttgggcattt
     9241 atctaccaat gtctgatctt tcagaccaca gtttccacat ataggtactc tcataattaa
     9301 tttaaatgaa ttagtcatgt tagatatctt ctcaatattc ttcttaattt cagttatttt
     9361 ggagccattt ggaattttca cctgtgtgaa taaaccacca ttgagagttt ttgccatacg
     9421 gttacattca acaatttgag gacttttgat agtaagatct gccagagtag tcgcatcaat
     9481 aacaactcct tctgtataag tctcattctc agtaattttg agtatggagt ttttaccata
     9541 tttttcttca tctagagaaa taaacctacc agaaccatca cttttagtca tagttactaa
     9601 cacatcagta ccaaggtctt tagctttctt taatgcaata tttactgatg tttcgatcac
     9661 tttgtgtaat atttcatgtc cttgtttatc atccttaaac ccaagtatac caaacacagc
     9721 ttcacgtaaa cccactaaat tgataactaa agatgttgaa catctttgca tatactgtgt
     9781 attattggca agaattggat tcatacctaa cctaacaagt tcagagatat tttttttacg
     9841 taaattcata gatactaatg caggtttcat taataatgca agtcttgctc tgaaataagt
     9901 ttcatctttg tttgattcaa atgcaagtcg tggtaagtta attgatagtg agcctaaatg
     9961 tagagttgaa gaacttgtac tttttaatct agtaacaccg ttttgtgata tgttatgttt
    10021 tgcaaatatt atttctcctc caagagaaat gatttcagat agtatgtttg atacatcagt
    10081 gatctttcct ttttcataat tgataacaag acctatcctt ggacgaggtg tgagctccac
    10141 atatgtccta taggcagaaa gtacaacgtt tacgattttt tgttcgacac cgagtggtag
    10201 tgtaaatgat acaattgtcg gagttttatt gaatttgaat gctgttgaag acataataaa
    10261 tgaattaaca agtttttctt ctaattcaga taagttcttg gaatattttg atagtaataa
    10321 aactaaacca tccataatta cctcctgaga agcctcttta gcaattaatg atattatcat
    10381 ggataatgca gaagatagat tactcaatgt tttgatagat gtaagtttgc ttacactcaa
    10441 agcttttccc ttcagatcaa taccatcttc gattaattct tttatgttga tgaaaatact
    10501 atctggtaat aatgaccata atcctggacg tgtaatatga agatcacctg atagatgctc
    10561 atcagctata tcttttggaa gtgtgtttgt aagaagatat tcagcaaaga cagtttgacc
    10621 tgtattaaat aataaactct ctatgccact atctagattt tctgcatttg aaattgtttc
    10681 ctgaacctca aatgcgggaa gtccaactcg cgcaagttta tgtctatatt cttcgtaacc
    10741 atgctccaaa agcactgaat taaccaattc acgaataagt gaaccagtta ggtatgcagt
    10801 ttggtatttg tagatacgat tctctacttc ttcagttatt ttttgtgcaa gttctaacgg
    10861 taaattccct tcacgtacta atgattgaat tattttatga gagttaaatt cttcaataga
    10921 atcatgtgaa gttcttacat acattttacc actttcaata attgatcttt cttcgatctg
    10981 tcttgctaga cttaatacaa gtttacccag atttgtaatt gtatatcttc tctctgattt
    11041 atttaatgct acaagtgatt gccttaataa tttccttaaa tggtaagcaa atttaccact
    11101 ttcttttttt gatttgaatc ctgccaatga tttgagttca gagtaagtaa gcggtccttt
    11161 tgagtttaag attcgtaata tatcaattcg gtttgggctt gccataacag aaaatatcat
    11221 tcgtacacgt tttgaactgg attgaagtgc tccggtttct gatggttcgt ctaaatcatc
    11281 agcatcaaga cctgctccat ctaactcatc atctaaatct aaatcagcgt ttagatctag
    11341 ttcatcttct aatgctatac tctctttact ccccaatatg taacaaaata aattcgagta
    11401 attaagactt attgctcata tggcgtacag attatttttt ttctatatct aatgaaaatt
    11461 cactagttga aagtttctgt ccacatgaag gacatttatt attgtaatgc tttagaacat
    11521 ctttaattgg ttttagcatt tgcattgtag aaatattttc tccacatttt ccacatttaa
    11581 tatcaacaga catacaaaaa aattaatttc gtcacgtatt agttttttta aacattttat
    11641 tctatcctag actaaattct gtttatcttt tttcaagtac taatccacgt ttatcaaaat
    11701 ctattatttt gactgctgta ccaattggaa gatctgctgg tttgccaata ttagccaaaa
    11761 aaccggatat attcaaatca tgaccttcga ttttcataac tacccatgca tatggtgaaa
    11821 aagtttcaaa gcctgagggt ggaactgtca taattgtata agtcacaatt acaccattac
    11881 cgtccaatat ggtattttca aattcctttt ttccacattt tttgcagaaa tacacggtta
    11941 ccaaattcaa gtcattacaa ttcttacatt tacgagctag aatcttacct tgttttgcat
    12001 ttgcaataaa ttcatctttt attgtcaatt tacacacttt gaaatatatg aacagcacaa
    12061 ctagcgccag ttgcaccaaa attctgagtt aagccaatct ttgcgtcatt tacagttcgt
    12121 tcacctgcgt ttcctgttaa ttgatcataa acttcaacaa cttgacctac acctgttgca
    12181 cctattggat gaccttttga tttaagaccg cctgatgggt taattggtag ttgaccgttt
    12241 aatttagtta caccattacg aattgcatct attcctttgc ctttttcaaa gaaaccaaga
    12301 tcctcagtgt caacaacttc cgctatagta aaacaatcat gaacttcggc aaaatcaata
    12361 tcctttgatg tgatattcgc cattttgtaa gcagcatctg ctgcaatctt tgtacttggt
    12421 atagtagtaa tatgatctct accttgcagt gctgcaggtg atccacccct acctgaacca
    12481 ataacttcaa cataatcacc accttgttgc tttgcaaatt tctcactaca aattataact
    12541 gcacttgcac catcagaaaa tggacagcaa tcataaagtt tcagtgggct tgcaactaca
    12601 gcagaattca ttacatcatc aatagtaatt ttttttctta aatgtgcttt aggatttaac
    12661 aatccgtttt catgattctt aacagctact gctgcaaaat cttcctcaga tgcattaaac
    12721 tctgtaatat aagctcgagc catagatgca aataatccag ggaatgagac acctgcctga
    12781 ccttcataaa aaaaatctga gcaatatgaa aaatatgtgg ttgtccattc tgttcctgta
    12841 tgtgtaactt tttcgactcc agttactaac actacatcat aaaatccagc agcaacattt
    12901 gcgtaagctt ctctgaatga tacagaacca ctaccgcaag ctgattcaat agaaagtgac
    12961 ggtttgtctg atattcctag gttactcatt attacaggtc caagatgtac ttgtttatca
    13021 gcaacaccaa aaacattaga aatgtaacca gatttaattt cctttgattc tattccagca
    13081 ctttcaattg cacttactga tgcttgaagt gtgatatcag ttatactatc ttctaattta
    13141 ccatatttgg tacttccagc acctaaaaca catacttttt ccacaaaaaa ttctgacctg
    13201 ttccctataa aaattcttca aaaaatttca taagggttga tcgatttaat aacatatgaa
    13261 aaaaagaatt caatctttac cattaacaaa aacgaaaaaa tctattagtg taaccaagcc
    13321 tacaaagaaa aaaataaaaa ttttaaaaaa tgaattagag caatatttag attctaatgg
    13381 ttacatctct tattctacaa aaaagaaaaa atatgttatt ttaggcacaa attctccaaa
    13441 gaatggaatt gttggttgcc cgcaatgtgg gattggtcaa ttgatgatta taaaatcacc
    13501 aataactaag aagaggttta ttggttgttc taattataat aatggatgta ctgcatcatc
    13561 tccacttttg caaaaagcta cactacgagt aattaaggca aaatgtcagt tatgcaaatg
    13621 gcctattgtg gtttttagat atgctagaaa ccagaactgg aaaagacagt gctcaaatat
    13681 agaatgtaaa tcaagaaaaa ccaaaatcag tgtataaatc tgttctacga tttttcaata
    13741 atggtaattt ttttctaatt ttctgtataa ctgcagaaga gatgtttacg tatcccaatc
    13801 cttgtcgttt tttcatatcc agaatgattt taccataagg atcaaccact aaacttcttg
    13861 ctgaatatat atttccaaga tggtttggag caataacata acatccattt tcaacagcac
    13921 gagatttatt taatgtaaac caatgctcct ctttcattgg acccttgacc caagcagaag
    13981 gcacaactag tatttcacta ccggatgatg ctaatattct agatagttct ggaaatctta
    14041 gatcataaca tatcatcatt cccaattttc caactgaagt ttttgttgtt ggaggaagag
    14101 tttttcctgc cgacagtttt gacgattcct tgaatccgag tgcatcaaaa aggtgtattt
    14161 ttctatactt ggcagaaatt tttccatttt tatttattat gaatgaagta tcaaaaaccc
    14221 tatctttttt tctgcttttt tcatacatag ttccaattac aattatggaa ttcttccttg
    14281 cgcattctct tatagttgat acaaatttac cattaattgt ttcagcttgc cgtgcaactt
    14341 gttttgcaga ttgtgttctt ggtgtataaa acatcatgta ctctggaaat gcacaaagat
    14401 ctgcatgatt ttttgctgct tgtttaatat aatctaaaat ctttggaaga ttttttgttt
    14461 tatcagttga agccttgaat tgtactatgg caactttaga catatgtttc ataatattga
    14521 tcaaaataaa aagatagtta gagttggctt cctgctacaa accaattctc ttttggattc
    14581 tcatcgaata ctacaatcac atgttcctcc tttgttttta gaacttcaac tgcagatttt
    14641 gtaatagact ttgcaaaatc atttttttgc tgttgagttc tgcctggata cattgataca
    14701 gttatcagtg gcatgaagat ttttttgtaa tctacaattt atgttgatgc tatctgtaat
    14761 ctcccctata gccataacgt gtaccataca tagacgctga accgcgagat tttttgtaga
    14821 tatatccaac agcaagtccc aatacgaagc ctccaatatg cgctaaatat gctacaccac
    14881 ctcctgcaac accaaaccct cctatgaaaa atggtaacaa attttgaaaa attagccaaa
    14941 atggaagaaa atacatagcc ttgatgtgaa gcattctcca gaaaaatccc aacatcaaaa
    15001 atgtcttaac ttttgcatgt ggaaacatta caaggtaagc tcccagtaca ccggatattg
    15061 cgcctgatgc accaaccgca ggaattccag ttgatgtatc gcccatgacg tgaattaacc
    15121 ctgcacctat tccccataac agatagatgc ctagatattt tactcgtcca aactttgctt
    15181 caatgttgtc accaaagatc caaagaaaca acatgtttcc accaagatgc atcaaacctc
    15241 catgcatgaa aactgaggat aataatgtaa gctctggaat tgcaggacaa ttaatgatat
    15301 taattcctgt atcaatacca cttatttgtc cggtaataca accaggtact gcgccccaat
    15361 tcaagaacat attcattgct tgacgattgc taaattcaaa aaattgtcca gttacagcta
    15421 cttcaaaaag gaaaataatc acattaatcg caattaatgc atatgttagt tttggtttga
    15481 atcctggtgg atgaggattt tcatccccta ttggtaacat atctgattat ctcacacagt
    15541 atatcaataa taatatttca tgaaatgata ttacgaaacc gtcatgattc cgtttgatat
    15601 taaccattga agtccagaaa caaatgcaga atcaggaata tctccactag cccaccatcc
    15661 tgcattattt ttgatccatc ctggaattac atcgtcagat c
//
LOCUS       DASCPJ010000044         1623 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000770152, whole genome shotgun sequence.
ACCESSION   DASCPJ010000044 DASCPJ010000000
VERSION     DASCPJ010000044.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000770152"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="QGF01_02195"
     CDS             <1..576
                     /locus_tag="QGF01_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HJN19741.1"
                     /translation="PIEFSHQNIKSIVNQREVGVDTKSYANALKASLREAPDVILVGE
                     IRVRETMEAALELSNTGHLALSTLHANNAGQAIERVINMFPQSMHKQLLMDLSLNVRG
                     IISQRLVKAKDGKRCAALEILVMTPHIQDLILKGDIEEIKIAMEESGKKGMQTFDTAL
                     HNLYKEGRIELEEALNNASSRTNLEAKINFG"
     gene            complement(617..1504)
                     /locus_tag="QGF01_02200"
     CDS             complement(617..1504)
                     /locus_tag="QGF01_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HJN19742.1"
                     /translation="MARPKTRNTLYVEQAKLISQDIYDNDQYHMILQAPETSMHAKPG
                     HFVHMQCSPTIYMRRPMSIMRSTPKNNTIEILYKVHGEGTNALSKKKVGDEIDLIGPI
                     GQPFKIKNYKKYPLLIGGGVGIPPMIFLAEHIKDTTKNLSPLILMGSEIPFPFKIQPS
                     KIMLKEIPADVIASMPLLDDWGMPSRLTSLNDYAGCFNGYVTELANIWLEKLDDDQRQ
                     EVEIFSCGPTPMLKAVSELANKYDLPCQVSLEEYMACAVGGCAGCTVLIKTENGNAMK
                     RVCVDGPVFEAKSIVQFQN"
BASE COUNT          517 a          284 c          298 g          524 t
ORIGIN      
        1 ccaatagaat ttagccatca gaatattaag tcaattgtca atcaacgaga agttggtgtt
       61 gatacaaaat catatgcaaa tgctctcaaa gcctcactac gcgaagcccc agatgtaata
      121 ctagttggcg agattagagt tagagagaca atggaggcgg cacttgaatt gagtaataca
      181 gggcatctcg ctttatctac ccttcatgca aataatgcag gacaagccat tgaacgtgtt
      241 attaatatgt tcccacaatc tatgcataaa caattattaa tggatttatc actgaatgtt
      301 cgaggcatta tttcacaacg acttgtaaaa gcaaaagatg gtaaaagatg tgctgcttta
      361 gaaatattag tcatgacccc acacatacaa gatctaatct tgaaagggga tattgaagaa
      421 atcaaaatag ctatggaaga gagcgggaaa aaaggtatgc agacatttga tacggcgtta
      481 cataatttat ataaagaggg gcgtattgaa ctagaagaag cgttaaataa tgcaagttca
      541 agaacaaatc ttgaagctaa aataaatttt gggtaaaaat cattttttta ttcattactg
      601 aattatttta taacgcttag ttttgaaatt gaacgatact ctttgcttca aatacagggc
      661 catcaacaca aacacgtttc attgcatttc cattttcagt tttaattaat actgtacagc
      721 cagcacatcc gccaactgca caagccatgt attcttcgag tgatacttgg cagggtaaat
      781 catatttgtt agcaagttca cttactgcct ttagcattgg tgtaggccca caggagaata
      841 tctctacttc ttgacgttga tcatcatcaa gtttttctaa ccatatattt gcaagttcag
      901 ttacataacc attaaaacaa cctgcataat catttaagct agtaagacgt gatggcatac
      961 cccagtcatc taataaaggc attgacgcaa tcacatcggc aggtatttct tttagcatta
     1021 tttttgatgg ttgaatttta aagggaaacg gaatttcaga gcccatcaat ataagtggag
     1081 ataaattttt tgttgtgtcc tttatatgtt ctgccaaaaa aatcatcggc ggaataccaa
     1141 caccaccacc gataagtaga gggtattttt tgtagttttt tatcttaaag ggttggccaa
     1201 ttggccctat caaatcaatt tcatcaccaa cctttttttt agataatgcg tttgttcctt
     1261 caccatgaac tttataaagt atttcaattg tattgttttt aggagtgctg cgcataattg
     1321 acattggtcg acgcatgtaa atagtcgggc tgcattgcat atgaacaaaa tgaccaggtt
     1381 ttgcatgcat agatgtttct ggagcttgca atatcatatg gtattggtcg ttatcataga
     1441 tatcttgtga tatcagtttt gcttgttcca catataatgt gttacgtgtc tttgggcggg
     1501 ccattagatt aatctgtact aaaaaccact tcaccattta gtaacgtatg agttactttg
     1561 ccttgtagtt cccaaccatg aaagggtgta ttttttccag tactgagcat attattttta
     1621 tca
//
LOCUS       DASCPJ010000045        10370 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000770619, whole genome shotgun sequence.
ACCESSION   DASCPJ010000045 DASCPJ010000000
VERSION     DASCPJ010000045.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000770619"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="QGF01_02205"
     CDS             <1..903
                     /locus_tag="QGF01_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HJN19743.1"
                     /translation="DDVPAIFSAYDKKIEQLDVGKSGKVGLLRLKLEENSDKNKTVIT
                     DQYSQVPLYTQKALYYDEKLPNMAHLFIMSPSGGILQGDRYRMDISLANNAVSHITTQ
                     GATRIYKMNSNYATQLININVGKNCYLEFIPDQIIPFRNSRYYQKVSLNVDPSATLIY
                     SEIIVPGRVAMGELFSYDLCYLKTIAQNTEKKIKFIDNCMLKPKNQNMNTLGMFGNHT
                     VLASIYILTKNEHIQILNKKINSVIKNNDEVSGGSTILPNNSGLLVRLMCNSSEIIKT
                     EIYDVVRIVRKEILDAEFTDIRKT"
     gene            complement(906..1463)
                     /locus_tag="QGF01_02210"
     CDS             complement(906..1463)
                     /locus_tag="QGF01_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJN19744.1"
                     /translation="MLLPSLETIKQKREKLHISQKKLAVMTGVSTSMINQIESGRSKP
                     SYDTAKKIFENLANLEGQSSSHVAGEICKSPIIKMKITQTVKEAIKKMESLKISQIPV
                     FDSAEPVGIVSEEGLVRKLADSNISQWKQTHLKDVMTAVPPIINYNTPANTLGPLLQF
                     TKCILVSKNSKIIGIITATDTLKMI"
     gene            complement(1517..4168)
                     /gene="ppdK"
                     /locus_tag="QGF01_02215"
     CDS             complement(1517..4168)
                     /gene="ppdK"
                     /locus_tag="QGF01_02215"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HJN19745.1"
                     /translation="MKKFRPVYAFEEADFKNRMLLGGKGAGLSEMTHLKLPVPPGFTI
                     TTQVCNQYYENGKKLPKDVMPLVMKNIVKMEKKTNKKWNSTKNPLLVSVRSGAAISMP
                     GMMDTILNLGLNEKTVEGFAKQTNNPRFSWDSYRRFIQLFGKVVFGVDDKKFDNVLNT
                     TKKKQKVKDDSKLNVKSLKKIVLEYKKICEKHTKRKFPDSPNEQLELAIEAVFKSWMG
                     ERAVVYRRQNKVTRDIANGTAVNVVTMVFGNMGDDSATGVVFTRNGHNGKKELEGEYL
                     TNAQGEDVVAGVRTGKNIEMLKKEMPRSYKELNNACTKLEKHFKEVQDIEFTIEQGKF
                     YLLQTRNAKMAAQGYVKTSVDMIKEKLIDRDRSLTRIPAEQLEALLHKTIDESKIKQF
                     KQLAKGIAASPGAANGIAVFDVKKAIELGESGKKVILIRKETKPEDVPAFFSSEGILT
                     SLGGKSSHAAIVSRGMGKSCIVGCNELKIDEERNRCNANGITVKENDVVTIDGTTGAI
                     YIGNVPTVKPKKTKDFETILEWAQKTKKLGIRANADTPNAAKLAREYGAEGIGLCRTE
                     RMFNEENRIGMFVKMIMAKSVEEREKVLEKLQKLQKSDFIAILKAMEGYKVTIRLLDP
                     PLHEFLPNPETLMDKIYKKKDKSEKTMRVLDRARDLAETNPMMGHRGVRVGITYPEIY
                     RMQIKAVFEAVAQLTKSKVDAKPQIMIPQVGSIAELNYIKNIYDEVKKEMQKKFKMKF
                     NINFGTMLEVVRACLTANELANTAEFFSFGTNDLTQGVFSFSREDVEGKFLGDYMDKG
                     LVEISPFQSIDENGVGYLMQIGIKQGRHVQKTLEIGICGEHGGDPNSIKFCHAVDVSY
                     VSASPYRIPTAILAAAQAVIEQKKSKK"
     gene            complement(4194..4850)
                     /gene="tpiA"
                     /locus_tag="QGF01_02220"
     CDS             complement(4194..4850)
                     /gene="tpiA"
                     /locus_tag="QGF01_02220"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HJN19746.1"
                     /translation="MFIINCKNYEEIAGEKIIKLAKIAAKISKKYKIKIAIAPPHHLI
                     PLITKFGIIVLAQHLDDKKIGSTTGFMIPEIIKKSKIDGSIINHSEHRITESEIKNLV
                     KRLKKLKLKTVVCVKNVSEAKKYAKINPTFIAIEPPELIGTGRAISTEKPQLITNSIN
                     AVQSAKNSTKLLCGAGIVSAEDVSRAVELGSKGILVASGVIKAKNWESILSDFSRGLV
                     "
     gene            complement(4851..5135)
                     /locus_tag="QGF01_02225"
     CDS             complement(4851..5135)
                     /locus_tag="QGF01_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HJN19747.1"
                     /translation="MELDETTMAVIGILAGILILSGWVPQIMKGYRTKKLDDVSRYLM
                     ILIFIGAALWLVYGMALDDVYIMGVNIAAMILTMTVLSMKLKYEKKPDYT"
     gene            complement(5141..7699)
                     /locus_tag="QGF01_02230"
     CDS             complement(5141..7699)
                     /locus_tag="QGF01_02230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase I"
                     /protein_id="HJN19748.1"
                     /translation="MTEQITNNNLESTSNLLLISANYNSQQKAVVLKFYDPTSHKIVL
                     WTDKTGHKPYCFSKMSPAELDFLSERSDVISIKEVTKTDLLEDKTITVSKILVSDPLA
                     IGGTQTDKSIRNVVEAWEADIKYYENYLYDNSLIVGKYYRIENDKLTPHNFKISDETR
                     LSLKSMLWDKLGELNIPDHEQFMKNVSDWADLLNQPIPKLRRISLDIEVESEEKNRLP
                     DAKTAVGRVTAIGFESSDNLKQVFVLRRNDISEGTNDLAPDIKVRFYDEDNEKGMIED
                     VFNLIKDYPLLITYNGDGFDLPYLYNRAERLGIDKNTNPLYMMKDSATLKEGVHLDLY
                     RTLSNRAFQIYAFGQKYTDFSLNSVSKGLLNEQKLDYGVEIDKMTLYQIGKYCQNDAY
                     LTYKLTSFNDDLLMNLLVVISRIGRMPIDDISRMGVSQWIRSLLYYEHRKNNMLIPRR
                     EEIDKRSTDVTNDAVIKDKKYRGGLVVEPKEGIHFDVTVMDFASLYPSIIKVKNLSYE
                     TVRCSHTECKKNMIPQTNHWVCIKKNGLTSVLIGSLRDLRVNYYKTMSKNQNLTNEER
                     QLYTVVAAALKVILNASYGVMGAQIFPLYFLPAAEATTATGRHIILDTIEKCRGLNVD
                     VLYGDTDSIFVKNPTEQQMQTIISQAKNNHGVELEVEKEYRYVVLSDRKKNYLGVTKN
                     GKVDVKGLTGKKSHTPAFIKSLFYDLLDILSEVKTSDEFKNAKSKISSKIAECSRKIQ
                     AKEIPLSELAFNVMLSKSLNEYTKTIPQHIRAAKQLESVREIKKGDRISYVKIINKPG
                     VRPVELAKQSEIDSSKYMEFMQSTLDQITSSMNMDFDTIIGKPRQTGLDQFFWN"
     gene            complement(7701..8216)
                     /locus_tag="QGF01_02235"
     CDS             complement(7701..8216)
                     /locus_tag="QGF01_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HJN19749.1"
                     /translation="MLSESFERPTWDEYFMLQAELAKLRSNCLTRQVGAVIVRDHRQI
                     SMGYNGTPPGIKNCYEGGCKRCQDRMEGKIKSGESLDRCLCNHAEANAIMHCAILGIE
                     AGNKDTVLYSTFVPCLECSKMAITIGIKRIICLDSYPETDYDLLNQAGAKVEKLEKTK
                     IQYWSKSLQNI"
     gene            8316..8453
                     /locus_tag="QGF01_02240"
     CDS             8316..8453
                     /locus_tag="QGF01_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19750.1"
                     /translation="MPKCCICKKIEGKIKIKEGNPAYKGKPICAECQGYRKLLLEDHK
                     H"
     gene            8534..10021
                     /locus_tag="QGF01_02245"
     CDS             8534..10021
                     /locus_tag="QGF01_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HJN19751.1"
                     /translation="MNFIEHKLIKPNSIEIREYQTNLANDVKNQNCLIVLPTGLGKTT
                     IALQVIVDYMQNGTGGVLFLAPTRVLVNQHYEFLKENLLIADIGIVTGEDLLSKRKKK
                     WMNSVVCATPEITKNDFARNMVSPNQFALVVFDEAHRTVGDYAYSIIAEYFQNTDTRI
                     LAMTATLPSEKEKAKEIVTRLRIKNLIERSDESPDVKPYIQKTETEWVRVDLTKEMKA
                     IQMCIKLALEERYKELRKNGLRLSDNKSLSQLLNARQYVLTQNRRSAKPLFTAIRITY
                     ALNIFEAHGITPFLKFCDRTKEKKGAGIKDLFETDPNFTRAISLAKNQQSNGLEHPKI
                     DRLVDILHDVESKVLIFSSYRDSVNVIHQKLLGMNISAEILIGKSGETGLKQKKQIET
                     VQRFRDGVTKALIATRVGEEGLDISEVNLVIFYDNVPSSIRFIQRKGRTGRKDKGRLI
                     VLIAKNTIDETYYFIGKRKIESAKNMGDKMIKHLGKDEKVLDDFF"
     gene            complement(10022..10300)
                     /locus_tag="QGF01_02250"
     CDS             complement(10022..10300)
                     /locus_tag="QGF01_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJN19752.1"
                     /translation="MANKIKCSHILVKKQSESIEILDKIKKGEKFGKMARQFSTDSGS
                     AKRDGSLGYFGRGKMVKEFENAAFALQTGEISEPIKTQYGYHIIKRLS"
BASE COUNT         3161 a         1830 c         1550 g         3829 t
ORIGIN      
        1 gatgatgttc cagcaatatt ctcagcgtat gataaaaaaa tagagcaact ggatgtaggc
       61 aaatcaggca aagttggact tttgaggtta aaacttgaag aaaattcaga taaaaataaa
      121 actgtaatca ctgaccaata ttctcaggtt ccactttata cacaaaaagc actgtattat
      181 gatgaaaagc ttccaaatat ggctcatttg tttatcatgt caccatcagg aggcatatta
      241 caaggtgacc gatatcgaat ggatatttcc ctagcaaata atgctgtaag ccatattaca
      301 acacaaggtg caactaggat ttacaaaatg aattctaatt atgcaacaca gctgatcaac
      361 ataaacgttg gaaagaattg ctatttagaa tttataccag atcagataat accatttaga
      421 aattctagat attatcaaaa ggtatcatta aatgtagatc cttctgcaac attgatttat
      481 tcagaaataa ttgtaccagg aagagttgca atgggcgagt tgttttcata tgatctatgt
      541 tatcttaaaa caattgcaca aaatactgaa aaaaagatta aatttattga taattgcatg
      601 ttaaaaccaa aaaatcaaaa catgaatacg cttggaatgt ttggtaatca tactgttttg
      661 gcatcaatat atattttgac aaagaatgaa cacatacaga ttctaaataa aaaaataaat
      721 tcagttataa aaaataatga tgaggtttca ggtggttcta ctatattacc aaataattct
      781 gggttattgg taaggttaat gtgtaactca tctgaaataa ttaaaactga aatatatgat
      841 gtagttagaa ttgtgagaaa agaaatactt gatgcagaat ttactgatat cagaaaaaca
      901 taaaattata tcatctttag tgtatctgtt gcagtgatga taccaattat ttttgaattt
      961 tttgaaacaa gtatacattt ggtaaattgt agtaatggcc caagtgtgtt agctggtgta
     1021 ttgtagttta ttatcggtgg aactgctgtc attacatcct ttagatgtgt ttgtttccac
     1081 tgggaaatat tagaatctgc taatttcctt acaagacctt cttcactaac tatcccaaca
     1141 ggttcagcac tatcaaaaac tggaatctga ctaattttta aactctccat tttttttatt
     1201 gcttccttaa ctgtttgtgt aattttcatt tttattatag gtgatttgca aatttcacca
     1261 gcaacatggg atgatgattg accctcaaga ttagctaaat tttcaaaaat tttttttgca
     1321 gtgtcatacg atggtttact tctacctgat tctatttgat ttatcatgga tgtactcaca
     1381 ccagtcatca cagccaattt tttttgactg atatggagtt tttcacgttt ttgtttaatg
     1441 gtttccaaac taggtagcag catatcatta ttacttaatt ttcattataa aaagttaagt
     1501 attaattaat agtatattat ttttttgatt ttttttgttc aataacagct tgtgctgctg
     1561 caagtattgc agtaggtatt ctgtatggtg acgcactgac atatgatacg tctactgcat
     1621 gacagaattt gatagaattt gggtcaccac catgttcccc acatatacca atctctaggg
     1681 ttttttgtac atgtctacct tgttttattc caatttgcat tagataccca acaccatttt
     1741 catcaattga ttggaatgga cttatctcca caagaccttt gtccatgtaa tcaccaagga
     1801 attttccttc tacatcttct ctgctaaagc taaatacgcc ttgtgtcaaa tcatttgttc
     1861 caaaactgaa gaattctgcg gtgtttgcca attcgtttgc tgtaagacat gcacgtacaa
     1921 cctcaagcat tgttccaaaa ttaatgttaa atttcatctt gaattttttc tgcatctctt
     1981 tttttacttc gtcatagata ttttttatgt aatttaattc tgcgatactt cctacttgtg
     2041 gaatcataat ctgtggtttt gcatcaactt tgctttttgt aagctgagct actgcttcaa
     2101 atactgcttt aatttgcatt ctatagatct ctgggtatgt gattcctact ctgactcctc
     2161 tatgtcccat catcggatta gtttctgcca aatcacgtgc acggtctaat acacgcattg
     2221 ttttttcact tttgtccttt ttcttgtaaa ttttatccat taatgtttct ggattgggta
     2281 aaaattcgtg tagtggtggg tctaagagtc tgattgtgac tttataccct tccattgctt
     2341 ttagaattgc tataaaatca gatttttgta gcttttgtag tttttctaaa actttctctc
     2401 tctcttctac ggattttgcc ataatcattt ttacaaacat accaattcta ttttcttcgt
     2461 tgaacattct ttctgttcta cataatccaa ttccttctgc tccgtattct cttgcaagtt
     2521 ttgcagcgtt tggagtatca gcatttgcac gaattcctaa tttctttgtt ttttgggccc
     2581 actcaagaat tgtttcaaaa tcttttgtct tctttggttt tactgttggc acattaccga
     2641 tatagatagc accagtagta ccatctattg ttaccacatc attttccttt acagttattc
     2701 catttgcatt acatctgtta cgttcctcat caatcttcaa ttcattacac ccaacgatac
     2761 aggatttacc cattcctctt gatactattg cggcgtgtga tgactttcca ccaaggcttg
     2821 tcaaaattcc ttctgatgaa aagaatgccg gaacatcttc gggtttagtt tcttttctaa
     2881 ttaaaatcac ttttttccct gattctccta attctattgc ttttttcaca tcaaaaaccg
     2941 caattccgtt tgcagcacca ggtgatgcag caattccttt tgctaactgt ttgaattgct
     3001 taatctttga ttcatcaatt gttttatgca ataatgcctc taactgttct gcaggaattc
     3061 ttgtcaaaga tctgtctcta tcaattaatt tctcttttat catgtctaca gatgttttta
     3121 catatccttg cgcagccatt tttgcatttc ttgtctgtaa caaatagaat ttaccttgtt
     3181 cgatagtgaa ctctatgtct tgtacttctt tgaagtgttt ttctagtttt gtacacgcat
     3241 tgttcagttc tttgtacgat cgtggcattt ctttttttaa catctcaata tttttcccag
     3301 ttcttactcc cgcaacaacg tcttcacctt gggcgttcgt caaatattct ccttcaagtt
     3361 cttttttacc attatgccca tttctagtaa atacaactcc tgttgcacta tcatctccca
     3421 tattaccaaa aaccattgta accacattaa ctgcggttcc atttgcaatg tctcttgtaa
     3481 ctttgttttg tcgtctataa accactgctc tttcacccat ccagcttttg aaaacagcct
     3541 caatcgctaa ttctaattgc tcgttaggtg agtcaggaaa ttttcgttta gtgtgttttt
     3601 cacaaatttt cttgtattct aaaacaattt tctttaatga tttaacattt aatttactat
     3661 catctttcac tttttgtttt ttcttagttg tgtttaacac attatcaaac tttttgtcat
     3721 ctacaccaaa gacaactttt ccaaataatt gaatgaatct tctatatgaa tcccatgaga
     3781 atcgtggatt gtttgtttgt ttggcaaatc cttctactgt tttttcattt aaccctaaat
     3841 tcaaaattgt atccatcatc cctggcatag aaattgcagc acctgatcta actgagacta
     3901 gtaatggatt ttttgtagaa ttccattttt tatttgtctt tttttccatt ttaacaatat
     3961 ttttcattac taaaggcatt acatcttttg gaagtttttt tccattttca tagtattggt
     4021 tacaaacttg ggttgtgatt gtgaagccag gtggtacagg aagttttaga tgtgtcattt
     4081 cacttaaacc agcgccctta ccaccgagta gcattctatt tttaaagtct gcttcttcaa
     4141 acgcataaac aggtctgaat tttttcatga ggtatgctcg atttgagctg cttttaaacc
     4201 aatccacgtg aaaaatcact aagaatactt tcccaatttt ttgctttgat aacaccactt
     4261 gctactagga ttcccttgga accaagttct acagcacggg atacatcttc cgcagaaact
     4321 ataccagcac cacatagtag cttggtagaa ttttttgcag attgtacagc attgattgaa
     4381 tttgttatta attgtggttt ctcagttgat attgctctac cagttccaat caattcagga
     4441 ggctcaattg ctatgaatgt tggatttatt tttgcatatt tttttgcctc actaacattt
     4501 ttcacacaaa cgactgtctt gagtttcagt ttctttagac gttttaccaa atttttaatt
     4561 tcactttctg tgatccgatg ctcactatga ttaattattg aaccatcgat ctttgatttt
     4621 tttataattt ctggtatcat aaaaccagta gtacttccaa tttttttatc atctagatgt
     4681 tgtgctaaaa caataattcc aaattttgtg attagtggga ttaaatgatg tggaggtgca
     4741 attgcaattt taattttata tttttttgaa atttttgctg ctatttttgc aagttttatt
     4801 attttttcac ctgcaatctc ttcataattt ttacagttta taatgaacat ttacgtataa
     4861 tctggttttt tctcatattt taatttcata gataagacag tcattgtaag aatcatagca
     4921 gcaatattta cacccataat atatacatca tcaagtgcca ttccataaac taaccataat
     4981 gctgcaccaa tgaaaatcag tatcatcaaa taccgtgata catcatctag tttttttgtt
     5041 ctatatcctt tcataatttg gggaacccaa cctgataaaa ttaagatccc agctaatatt
     5101 ccaatcactg ccattgttgt ttcatctaat tccatataga tcaattccaa aaaaattgat
     5161 caagccctgt ttgtctgggc ttaccaataa ttgtatcaaa atccatattc atggatgaag
     5221 ttatttgatc aagcgtactt tgcataaatt ccatatattt cgaagaatct atttcacttt
     5281 gttttgccag ttcaacaggt ctaactccag gtttgtttat aattttgaca tatgatattc
     5341 tatcgccttt ttttatttcc ctaacagatt ctagttgttt tgccgcacga atatgttgtg
     5401 gtatggtttt tgtatattca ttaagcgatt tacttagcat gacattaaat gcaagctcag
     5461 ataatgggat ttctttagcc tgtatttttc gtgaacattc agcaattttt gaacttattt
     5521 tactttttgc atttttaaac tcatcagaag ttttcacttc tgataatata tctaaaagat
     5581 cataaaacaa tgatttgata aaagcaggag tatgggattt ctttccagtt aatcctttta
     5641 catcaacctt accatttttt gtcacaccta ggtaattctt ttttctatca ctgagtacta
     5701 catacctgta ttctttttca acttctaatt ctacaccatg attgtttttt gcttgactga
     5761 ttattgtttg catctgctgc tctgtaggat ttttcacaaa tattgaatct gtgtcaccat
     5821 aaagaacatc aacatttaat cctctacatt tttcaattgt atctaaaatt atatgtctac
     5881 cagttgcagt tgttgcctcc gcagcaggta agaagtataa tggaaatatc tgtgcaccca
     5941 taaccccata actcgcatta agtataactt tcagtgcagc agcaacaaca gtgtataact
     6001 gtctttcttc atttgttaga ttttggtttt ttgacatagt tttgtaatag ttcactctaa
     6061 gatctctcag tgacccaatt agtactgatg ttaaaccatt tttctttata caaacccaat
     6121 gatttgtttg cggtatcata ttttttttgc attcagtatg tgagcagcgt acagtttcgt
     6181 atgacaagtt tttaactttt attatacttg ggtacagact tgcaaaatcc attacagtta
     6241 catcaaaatg aattccttct ttcggttcaa ctactagccc tccacgatat ttcttgtctt
     6301 ttataacagc atcatttgta acatcagttg atcttttatc aatttcttct cttctaggaa
     6361 ttaacatgtt atttttccta tgttcgtagt atagcaaact acgtatccat tgtgaaacac
     6421 ccatcctaga aatatcatca attggcatcc tcccaattct gctaatgacg acgagtaaat
     6481 tcatcaacaa gtcatcattg aagctagtta gcttgtaggt aagataggca tcattttgac
     6541 agtattttcc aatttgatat aatgtcattt tatcaatctc tactccataa tctaattttt
     6601 gttcattcag taagcctttt gacacactgt ttaatgaaaa atcagtatat ttttggccaa
     6661 atgcataaat ttgaaaagca cgattcgata gagttctgta caaatcaagg tgtacacctt
     6721 cctttaatgt tgcagaatcc ttcatcatgt atagtggatt agtattttta tcaattccta
     6781 gtctttctgc acgattgtac aaatatggta gatcaaaccc atcaccattg tatgtaatta
     6841 ataatgggta atctttaatc aggttgaaca catcttctat cattcctttt tcgttatctt
     6901 cgtcataaaa tctaactttg atatctggtg ctaaatcatt agttccttca cttatatcat
     6961 ttcgtcgtaa aacaaatacc tgttttaggt tatcactaga ttcaaaaccg attgcggtga
     7021 ctcttcctac tgcagtttta gcatcaggca aacgattttt ttcttcggat tcaacttcta
     7081 tgtctaaact aattcttctt aattttggta taggctggtt tagaagatct gcccaatctg
     7141 aaacattttt catgaactgt tcatgatctg gtatgtttaa ttcaccaagt ttatcccata
     7201 acatactttt tagtgataac ctagtttcat cagaaatttt aaaattatgt ggagttaatt
     7261 tgtcattttc tatcctataa tatttcccaa caattagtga attatcgtat aaataatttt
     7321 cataatattt tatatcggct tcccaagctt caacaacatt tcttatactt ttgtcagttt
     7381 gtgtaccacc tattgctaat ggatcactaa ctaaaatttt tgaaacagtt attgttttat
     7441 cttcaaggag atcagttttt gttacttcct ttatactaat tacatctgaa cgctcagaga
     7501 gaaaatctaa ttctgctggt gacattttag aaaaacaata gggtttatga cctgttttgt
     7561 cagtccataa aacaatttta tgagatgttg gatcataaaa ttttagaaca acagcttttt
     7621 gttgactatt ataatttgca gaaattagta ataaattgga tgtgctttcc aaattattat
     7681 ttgtgatttg ttctgtcatt ctagatgttt tgtaatgatt ttgaccagta ttgaattttg
     7741 gttttttcta gtttttcaac cttggctcct gcctgattaa gtaggtcata atcagtttca
     7801 gggtagctgt ctagacaaat tatccgctta attcctattg tgattgccat tttactacat
     7861 tctaaacatg gaacaaatgt tgaatacaag acagtgtctt tattcccagc ctcaattcct
     7921 aaaattgcac aatgcattat ggcgtttgcc tctgcatgat tacataggca tctatcaaga
     7981 gattcaccgg atttaatttt gccttccatt ctatcctgac atcttttaca accaccttca
     8041 tagcagtttt ttatacctgg cggtgtaccg ttataaccca ttgaaatctg tctatgatca
     8101 cgaactatca ctgcaccaac ttgtctagtc aaacaattag aacgtaattt cgctaattca
     8161 gcttgtagca taaaatactc atcccatgta ggtctttcaa atgattctga caacatgatt
     8221 ggttttattt cctgtaatat aagataaagt tcatttattc atgcatcatg ctttgggaaa
     8281 actagtttta taagatgact tgatgaaaaa atcacatgcc taaatgttgt atttgtaaaa
     8341 agattgaagg caaaattaag atcaaggaag gtaatcctgc atacaaagga aaacctattt
     8401 gtgctgaatg tcagggatat agaaaactct tacttgagga tcataaacac tgaatgctaa
     8461 catacagtca acaatcataa ttcaaaacta taaaatcaaa acatatccat aatcaaatgg
     8521 tgaaatagac ataatgaatt ttatcgagca taagctaatc aaaccgaatt ctatagagat
     8581 tagggaatat cagactaatc ttgcaaatga cgtaaaaaat caaaattgtc ttattgtact
     8641 tcctacaggt cttggtaaaa ccaccattgc gttacaagtg attgttgatt atatgcaaaa
     8701 tggaacaggt ggggtgttat tcctcgcacc aactagagtt ctagtcaacc aacactacga
     8761 atttctcaaa gaaaatttgc ttatcgctga tattggaatt gtaaccggag aagatttgtt
     8821 aagtaagagg aagaaaaaat ggatgaacag tgtggtttgt gctacacctg aaataacaaa
     8881 aaatgacttt gccagaaata tggtttctcc aaatcaattt gctcttgtag ttttcgatga
     8941 ggcacataga actgtaggag attacgcata ttcgatcatt gcagaatatt tccaaaatac
     9001 tgatacacgt atactggcca tgactgcaac tcttccaagt gaaaaagaaa aagcaaagga
     9061 gatagtcact agattacgca ttaaaaattt gattgaaaga tcagatgaaa gccctgatgt
     9121 aaaaccatac attcagaaaa ctgaaacaga gtgggttaga gtagatctta ccaaggaaat
     9181 gaaggcaatt cagatgtgta ttaaacttgc attagaagaa cgctataaag aattacgtaa
     9241 aaatggttta cgactctctg ataacaaatc tctttcacaa ttactaaatg ctaggcaata
     9301 tgttctgact caaaatagac gttcagcgaa accacttttt acggcaatta gaattacata
     9361 cgcattaaat atttttgaag cacatggaat cacgccattc ctaaagtttt gcgataggac
     9421 aaaggaaaaa aaaggtgctg gtattaagga tctttttgaa actgatccta attttacacg
     9481 agcgataagt ttagcaaaaa atcaacagtc taatggttta gagcatccaa aaattgatag
     9541 actggttgat attttacatg atgtagaatc taaagtatta attttttcta gttatagaga
     9601 ctctgttaat gttattcacc aaaaacttct tggtatgaat atatctgctg aaattcttat
     9661 aggtaaatca ggtgaaactg gactcaagca aaaaaagcag atagaaactg ttcaaagatt
     9721 tcgagatgga gtgacaaaag ctcttattgc tacaagagta ggcgaggaag gattagatat
     9781 atcagaagtg aatttagtaa ttttttatga taatgtacca agctctattc ggtttataca
     9841 aagaaaggga agaacaggaa gaaaagataa aggacgtttg attgtactaa ttgcaaaaaa
     9901 tacaattgat gagacatatt attttattgg taaacgaaaa attgagtctg caaaaaatat
     9961 gggagataaa atgataaagc atctgggaaa agatgaaaaa gtactggatg atttttttta
    10021 gttatgataa acgtttgatg atatgataac catactgtgt ttttattggc tcagagatct
    10081 ctccagtctg taaggcaaat gctgcatttt caaactcttt gaccattttt cctctaccaa
    10141 aataacctaa actcccgtct ctttttgcac taccagaatc agtagaaaat tgcctagcca
    10201 ttttgccaaa tttttcaccc ttttttatct tatccaatat ttctatagac tcactttgct
    10261 ttttcacaag gatatgagag cactttattt tatttgccat ttcaacacta ttcgaaaagc
    10321 cttttccaag agatttagca atttttgctt tgtcctttat aaaatcacct
//
LOCUS       DASCPJ010000046         1633 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000783713, whole genome shotgun sequence.
ACCESSION   DASCPJ010000046 DASCPJ010000000
VERSION     DASCPJ010000046.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000783713"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            36..551
                     /locus_tag="QGF01_02255"
     CDS             36..551
                     /locus_tag="QGF01_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19753.1"
                     /translation="MGESSALELYAILQKANAEGGGRFVAMICDGIEKYKKNLATIGQ
                     EGPTQVSVQEANASGYDKVIWIHAQYTPQEPGIELIAKSLGIDKSKICVPDVKTAQEL
                     LTTQQIPESLSKDLEGDSKPLLVCMAGKTSLMAAKVLATKGVMTDSLIGGITQLPEGQ
                     GKQLSELIRQA"
     gene            556..747
                     /locus_tag="QGF01_02260"
     CDS             556..747
                     /locus_tag="QGF01_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19754.1"
                     /translation="MKKQILSGKKFIPICLIVFGIIIGMPFNFMAGMDGVEADMIWIF
                     VGMAMVGYGIYKLKKELEN"
     gene            complement(748..987)
                     /locus_tag="QGF01_02265"
     CDS             complement(748..987)
                     /locus_tag="QGF01_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19755.1"
                     /translation="MANQVLVHVKTSKGTWETTFQKSSRIKDVILGSRVHFRLPKETK
                     LVLCKEESPHDDFDPDRALVNYNVIDGDVLILKQV"
     gene            1046..>1633
                     /locus_tag="QGF01_02270"
     CDS             1046..>1633
                     /locus_tag="QGF01_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HJN19756.1"
                     /translation="MTFTHLPKGELPEIRQIKIDDVRHYETPDGTFISITSLLKNYTP
                     DGILSWRESVGDDVADHVMRTATVRGNKVHKLIENCLSNKPESDLVGNHGVLAAGLFQ
                     LMIPALDKIDQIRFLEKAIYSKRLRLAGTADCIAEYDGKLSIIDFKTASRKRDEINEN
                     YLVQTTFYSIAWEELTGEKIEQIVILTVTEDGVLDV"
BASE COUNT          540 a          290 c          357 g          446 t
ORIGIN      
        1 ttcttctcaa gttttttgta gaaaaaggtc atgacatggg cgaaagcagc gcactcgaac
       61 tttatgcaat tttgcaaaag gcaaacgctg aaggtggcgg aaggtttgtt gcaatgattt
      121 gtgacggaat tgaaaagtac aaaaagaacc ttgcaacaat tggacaagaa ggtcctacgc
      181 aggtatccgt acaggaagca aacgcaagcg gctatgacaa agtaatctgg attcatgccc
      241 aatatacccc acaggaaccg ggaatagagt taattgcaaa atctctaggc attgataaaa
      301 gcaagatttg tgtgccagac gtaaaaactg cgcaggaact actgacaaca cagcaaattc
      361 cagaatcact aagcaaagac ctggaaggtg acagtaagcc attgctggtt tgtatggctg
      421 gaaaaacttc gctgatggct gccaaggtac ttgcaaccaa gggcgttatg accgacagcc
      481 tgattggcgg aataacacaa ctaccggaag gacaaggcaa acaactttct gaattaatca
      541 gacaggcttg aaaatttgaa aaaacaaatt ttatccggga aaaaattcat accaatctgt
      601 ctgatagttt ttggaataat tattggaatg ccattcaatt tcatggctgg gatggatgga
      661 gtagaagctg atatgatttg gatatttgtt ggaatggcta tggttggtta tggaatttac
      721 aagctaaaga aagagttaga aaactaatca tacttgcttt aggatcaaga catcgccatc
      781 tattacatta taatttacca gcgccctgtc aggatcaaaa tcatcatggg gtgactcttc
      841 cttgcatagt acaagttttg tttctttagg caatcgaaaa tgcacccttg aaccaagtat
      901 tacatctttt atcctagaag atttttgaaa tgtagtttcc cacgttcctt ttgatgtctt
      961 tacatggact agaacttgat ttgccatcaa gaaaaaatac tcccaagaaa taaaatagtg
     1021 tttagatggc agaatgtcat aaatcatgac ttttacacat ctacctaaag gagaacttcc
     1081 tgaaatcagg cagataaaga ttgatgatgt aagacattat gaaacgccag atggtacttt
     1141 catcagtatc acatcacttt tgaagaatta cacgcctgat ggcatattat catggagaga
     1201 gtctgttggc gatgatgttg cagatcatgt gatgagaact gctaccgtta gaggaaataa
     1261 agtgcataaa cttattgaaa attgtctatc aaacaaacct gaaagtgacc ttgttggaaa
     1321 ccatggtgta cttgcggcag gtcttttcca gttgatgata cctgcactgg ataagattga
     1381 ccaaatcaga ttcttggaga aggcaattta cagtaaacgt ttgcgtctag ctggaacagc
     1441 tgactgtata gcagagtatg acggcaaact atccatcatt gactttaaga ctgcgtcaag
     1501 gaagcgcgat gagataaatg agaattatct tgtgcagact accttctatt ctattgcctg
     1561 ggaagaactc actggtgaaa agattgaaca gattgtaatt cttaccgtta cagaagacgg
     1621 agtgctcgat gtg
//
LOCUS       DASCPJ010000047         4582 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000809699, whole genome shotgun sequence.
ACCESSION   DASCPJ010000047 DASCPJ010000000
VERSION     DASCPJ010000047.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000809699"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="QGF01_02275"
     CDS             <1..276
                     /locus_tag="QGF01_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19757.1"
                     /translation="LRFDNGSFVSITEGKENIGSMSASITSGPVPTSTIIIPSKKESL
                     FLKLMSEQISSIIQGINIVSAFLPQEIDSNTAKILMTKVMEMIRNDR"
     gene            266..1600
                     /locus_tag="QGF01_02280"
     CDS             266..1600
                     /locus_tag="QGF01_02280"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HJN19758.1"
                     /translation="MTDDLRKYLQEIKKANDLIVIKKKVSTKYEIASITEKLEGSKAV
                     LFENVKGSKFKLVSNLVGTRARFMQAIGSKKSDINQKIVKAISSAKKPKISTNAKFFE
                     NSSKDISILPTVTHFQNESGPFITSSILYTQNQEKKSQNSSIHRLMPIGKNQFTIRMV
                     EGRDLHRTFMFAKNQGKDLPVAITIGVHPAITIACAFQAKWGKNELEIANSLLNNKLT
                     LTKCPGTGFLVPSTAEIVMEGKILRNKTHKEWMVEMLRTYDMPRPAPIIQIEKLYFRN
                     NPIYHDILSGYTEARLLMGMPIEAKLNGIMKKRFPQTKQVILTSGGANWLHAVVQISK
                     TKSTNVKKIINETFANHRSLKMVTVVDDDIDPTDAVAVEFAMATRFQADRDLVIIKNV
                     RGSSLDPSSDQKRLRTTKMGIDATIPGSKRPEGFKLGTIPKGKSNLKDYLKK"
     gene            1597..2313
                     /locus_tag="QGF01_02285"
     CDS             1597..2313
                     /locus_tag="QGF01_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HJN19759.1"
                     /translation="MNARYFIVIVLVSGFTAGTIHGAVNLAIVEPYLDEAIEIENQSL
                     IASGQVQDTSQFWVEYNSYRDWQKSGQILAGGILGMSIGALFGIIFAYSRNSLPKGHT
                     VKKTFVLAAIMWVTIFLIPFLKYPANPPTVGDADTVVLRAILYLSFIAISGFSAVAFS
                     RLYKKLENKKYLAFVGYAIFITAVFFIMPPSPDEVTAPMDLVNGFRTMSVMAVTTFWI
                     AEAIILGMLWQKYKTKLQEP"
     gene            2584..3660
                     /locus_tag="QGF01_02290"
     CDS             2584..3660
                     /locus_tag="QGF01_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HJN19760.1"
                     /translation="MALSPIIASSAYAESSYVEKLEFGAGLEETLGHFWAIEQNLDDN
                     NAVLALVHATHPVAELYDAMKPTLVATSPSLDAKVSNILEELGAKTDSRQVTRADADQ
                     AIVDAKEVVAIARSQVIGDELSNDTGFKLELMKTLLETSIAEYEEAISDGVIGEMAEF
                     QDGSAFVWRAGVIFEEIEGDIPQHEADEIEELFEDVNAAYDKRADPSEVSTLTGGILH
                     EIDEILGTEESEGDQLQGYVNNIVDLLTRADAAYPGDKDLALSLVTKAYLDNYEFLEA
                     PLVNAGEGELMEDIEHEMREELRNMIKRNYSASEVSLQIDNILAQMDTAASVLGVPSG
                     EGWKTTSGGVLYYSSDNVSVSVHS"
     gene            3808..>4582
                     /locus_tag="QGF01_02295"
     CDS             3808..>4582
                     /locus_tag="QGF01_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19761.1"
                     /translation="MRQIHSILFVFLVIFLIGTIPNVLSAVDIDLVTFYSVKEITQTS
                     LILAEINIQNGNNETGKQFIDFASQQFSNNLQELRQLDSNLTDEIHISLIDLQTQQIN
                     SDNQSQISSQLEKINELLSTIPEDVEYNPNVIVALLIIADQQYENFETNDDEFSYQIS
                     LGLIERANQIFYAGTDYDERQKIELESFFNDLFEQVKNKDSFSSVGTLISFIQRDLLG
                     TDVLTTAGIDNSSLYNIIRELYGELLIELDNGNYDKAEEI"
BASE COUNT         1627 a          746 c          842 g         1367 t
ORIGIN      
        1 ttacgatttg ataacggaag ttttgtttcc ataactgaag gaaaagagaa tattggatca
       61 atgtctgcat caatcactag cggtcctgta ccaactagca caataatcat tccttcaaaa
      121 aaggaatcac tttttttaaa attaatgtca gaacaaatat catcgatcat acaaggcatc
      181 aatattgttt ctgcttttct tccacaagaa attgatagta ataccgctaa aattttaatg
      241 acgaaggtaa tggagatgat taggaatgac cgatgaccta agaaagtatc tacaagaaat
      301 aaaaaaggca aatgatctga ttgtcataaa gaaaaaagtt tcaacaaaat atgagattgc
      361 aagtattact gaaaaacttg aaggctcaaa agctgttcta tttgaaaatg tcaaaggaag
      421 caaattcaaa ctagtttcaa atttggtagg aactagagct agatttatgc aagcaatagg
      481 ctccaaaaaa tctgatatta atcaaaaaat tgtcaaggca atttcttctg caaaaaaacc
      541 aaaaatttct acaaatgcaa agttttttga aaatagttct aaggatattt ccattcttcc
      601 aactgtaaca cattttcaaa atgaatcagg tccttttatc acatcatcta tactttatac
      661 acaaaaccag gaaaaaaaat ctcagaactc ttctattcat agactaatgc caattggaaa
      721 aaaccaattc acaattagaa tggtagaagg aagagatctt catagaacat ttatgtttgc
      781 aaaaaatcaa ggaaaagatt taccagttgc aattaccatt ggagtacatc ctgcaattac
      841 aattgcatgt gcattccaag caaaatgggg aaaaaatgaa cttgagatag ctaactcact
      901 tttgaataat aaactcactt tgacaaaatg tcctggtact ggttttcttg taccatcaac
      961 tgcggaaatt gtgatggaag gaaaaatttt acggaacaag acacacaagg aatggatggt
     1021 ggaaatgctt cgaacatatg acatgccaag acccgctcca ataattcaaa ttgaaaaatt
     1081 gtatttcagg aacaatccga tttatcatga tatcctatct ggctatactg aggcaagatt
     1141 actcatgggc atgcctattg aggctaaact taacggtata atgaaaaaaa gatttccgca
     1201 gacaaaacag gtgatcctta ctagtggtgg ggctaattgg cttcatgctg tagtgcaaat
     1261 ctcaaaaacc aaatcaacta atgttaagaa aatcattaac gaaacgttcg caaatcatag
     1321 atcattaaag atggtgactg tagttgatga cgatattgat cctacggatg ctgtagccgt
     1381 tgaatttgcc atggctacta ggtttcaggc tgacagggat ctggtaataa taaaaaatgt
     1441 gagaggttcg agtcttgatc cttctagtga tcaaaagaga ttacgaacca caaaaatggg
     1501 catagatgcc acaatacctg gctcaaagag gccagaaggg ttcaagctag gcacgattcc
     1561 aaagggtaaa tcaaacctga aagactattt gaagaaatga atgccagata ttttattgtg
     1621 atagtcctag tttctggttt taccgcaggc acaatacacg gtgcagtaaa tttagcaatc
     1681 gtagagccat atctagatga ggcaattgaa attgaaaacc aaagcctcat tgcgtctggt
     1741 caagtacaag atacatccca gttttgggtt gaatacaact cgtatcgtga ttggcaaaaa
     1801 agcggccaga tactagcagg tggaattcta ggaatgtcaa taggtgcatt atttggcatc
     1861 atctttgcct actcaagaaa ttccctgcca aaaggacaca ccgtaaagaa aacatttgtt
     1921 cttgccgcaa tcatgtgggt taccattttc ttgataccat ttttgaaata tccagcaaac
     1981 cctccaaccg ttggagatgc agatactgtt gtactgagag caatacttta tctttcattt
     2041 attgcaatct ctggtttctc agctgttgct ttttccaggc tgtacaagaa acttgaaaac
     2101 aaaaagtatc tggcatttgt tggttatgca atttttatca cagctgtatt tttcataatg
     2161 ccaccaagtc ctgacgaagt tactgctcca atggatttgg tgaatggttt tagaacaatg
     2221 tctgttatgg ccgttacaac attttggatt gcagaggcaa taatacttgg aatgctgtgg
     2281 caaaaataca agacaaagtt acaggaacca tgatgttata ataaaatggg aaaaaatgcc
     2341 gctagtaata cttagctgcc aataaagtgg cctgccagat caagcacaat actacattac
     2401 cactaacggc aattttctaa tcataaatta ctaattaaaa ataatgttga atgtaatatt
     2461 gtattatcaa aagttgtaac ttattcttag aataaagatt tagaatgatc agtgggttta
     2521 actattgaat taattaggat cactccatcg atgaaattaa aaatactttc attatttgtt
     2581 cttatggcat taagcccaat tattgcatcg tcagcatatg cagaatcatc atacgtagaa
     2641 aaattggaat ttggagcagg tcttgaagaa acactaggtc acttttgggc tattgaacaa
     2701 aacttggatg ataacaatgc agtattggca ttagttcacg caacacatcc agttgctgaa
     2761 ttgtatgatg caatgaaacc aacacttgta gcaacttcac catcactcga tgccaaagtt
     2821 agtaacatac ttgaggaatt gggcgccaaa accgattcac gacaggtaac cagagctgac
     2881 gcagaccagg caattgttga tgcaaaggaa gtagttgcaa ttgcaagaag tcaagttatt
     2941 ggagatgaac ttagcaatga tactggtttc aaattagaat taatgaagac attactcgaa
     3001 acatcaatcg cagaatatga agaagctatc tcagatggag ttatcggaga aatggctgaa
     3061 ttccaagatg gttctgcatt tgtatggaga gcaggtgtaa tctttgagga aatcgaaggt
     3121 gatattcctc agcatgaagc agatgaaatc gaggaattat tcgaggacgt aaacgcagca
     3181 tatgacaaaa gagcagatcc ttctgaagta tctactctaa ctggtggtat attacatgag
     3241 attgacgaga ttcttggtac tgaagagtct gaaggagatc aattacaagg ttatgtgaat
     3301 aacattgttg atctattaac tcgtgcagat gcagcatatc ctggtgataa ggaccttgca
     3361 ttgagtcttg taacaaaagc atatcttgat aactatgaat tcttagaagc accactagta
     3421 aatgctggtg agggagaatt aatggaagac atcgaacatg aaatgcgaga agaattacgt
     3481 aatatgataa aacgtaatta ctcagcttcc gaggtgtcat tacagataga taatatcttg
     3541 gcacaaatgg ataccgctgc aagcgttttg ggagtaccaa gtggtgaagg ttggaagact
     3601 acatccggtg gtgtattata ttattcatct gacaatgtca gcgtatctgt tcattcttag
     3661 tactaactac aaatacaaag acgcaattac tcctaaactt taagaaaatt atttttttca
     3721 tttagttttt catacacacg tattttctta ttctataatc ttagaatgag aacttagata
     3781 aaatattagg tgaaactaac gattcgaatg aggcaaattc atagcatact gtttgtattt
     3841 ttagtgatat ttcttattgg aacaattcct aatgttttat cagcagttga tattgatcta
     3901 gtcacatttt attctgtaaa agaaattact caaacgagtc ttattcttgc tgaaataaat
     3961 atacaaaacg gtaataatga aactggcaaa caattcattg attttgcttc tcagcagttt
     4021 tcaaataatc ttcaagaatt aagacaattg gattctaatc ttacagatga aattcatatc
     4081 tcattaatag atctacaaac tcagcagatt aattctgata atcaatcaca aatttcatct
     4141 caactcgaaa aaataaatga acttttatca actattcctg aagatgtaga atacaatcca
     4201 aatgtaattg ttgcattatt gattattgct gaccaacagt atgaaaattt tgagactaac
     4261 gatgatgaat tttcttatca gatttcactt ggactaatcg aacgagcaaa tcagatattt
     4321 tatgctggta ctgattatga tgaacgacaa aaaattgagt tagaatcatt ttttaacgat
     4381 ttgtttgaac aagtaaaaaa taaggattca ttctcatctg ttggtacatt aatctctttc
     4441 atacagagag atttacttgg aactgatgtt ttaactactg ctggaataga taattctagt
     4501 ctctataata ttattcgaga actgtacgga gaattattga ttgaacttga taatggaaat
     4561 tatgataagg ctgaagaaat tg
//
LOCUS       DASCPJ010000048         2364 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000812619, whole genome shotgun sequence.
ACCESSION   DASCPJ010000048 DASCPJ010000000
VERSION     DASCPJ010000048.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000812619"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="npdG"
                     /locus_tag="QGF01_02300"
     CDS             complement(<1..593)
                     /gene="npdG"
                     /locus_tag="QGF01_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HJN19762.1"
                     /translation="MKIGIIGGTGGMGKGFALRWCKKHEIIIGSRDAERAISAANEYS
                     DLAKDTYGNVDGKILGKDNISVAKESQVLILAIPYPNIDSICSQLLPEISNECVVISP
                     IVPMTKTDVGFECISIKEDKPFSHQAVEKYMNDKTKLVSAFHVISEKKLVNPTLSLDY
                     DIFVVGDDMNSVKVVNDLINEIEGLRPIYLGGGKLSYL"
     gene            650..1267
                     /locus_tag="QGF01_02305"
     CDS             650..1267
                     /locus_tag="QGF01_02305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJN19763.1"
                     /translation="MIIFLRHGQAENNTKRILAGRTEGVPLTKIGIEQAEQIGKYLKP
                     IGISAIYSSPIERANNTAKIVAESNSIDYKLDERLTELDMGKFTRMPYDEIFAKHGNV
                     FLKFYSDDPIISEHNVETFPHVQKRVMDMLDYTIKKHDQENVILVTHMDPIKSVIAKV
                     IDLKPLSLFELIIANCSLTIIKHHDEKLSLSAINTMDADRFSQDW"
     gene            1325..1582
                     /locus_tag="QGF01_02310"
     CDS             1325..1582
                     /locus_tag="QGF01_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19764.1"
                     /translation="MRTIWAPIILIVLFATPMIQLVFASETPGEYVNRAQEIWNLFFR
                     MMTIAFVVGGVIAGTIVWQVWRFRESNPKAKPTPYEDKGDL"
     gene            1585..2007
                     /locus_tag="QGF01_02315"
     CDS             1585..2007
                     /locus_tag="QGF01_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19765.1"
                     /translation="MGHSNWPEWIYVAVVIALLVYVGAEAWNIENLIDHAPEDAEIIK
                     VTAQQWFWSFEHADGTKEIGELHLKKDHAYKFEIHSKDVNHNFNIPEFLVLMDAVPGR
                     INTVWFSPNESGEFDIQCREYCGLIHYNMRAKLFVEGS"
     gene            2063..>2364
                     /locus_tag="QGF01_02320"
     CDS             2063..>2364
                     /locus_tag="QGF01_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HJN19766.1"
                     /translation="MVLTLAKPRPIWQIMFSTHHTDVGLMYLIFSIMALFIGGAMAIA
                     IRVELFAPGAQIIADAMTFNRLFTSHGTTMIFLFLLPSASAVGNYLVPIMVRYKDMA"
BASE COUNT          794 a          412 c          405 g          753 t
ORIGIN      
        1 agatatgata attttcctcc acccaaataa attggacgta aaccttcaat ctcattaatt
       61 aaatcattaa caactttaac tgaattcata tcatcaccta ctacaaaaat atcatagtct
      121 aacgataaag ttgggttaac cagttttttt tctgagatga catgaaatgc agaaaccagt
      181 tttgttttat cattcatata tttctcaact gcttgatgtg agaacggttt gtcctcttta
      241 attgagatgc attcaaatcc aacatcagtt tttgtcatcg gaacaattgg tgagatcaca
      301 acacattcgt tactaatttc aggtaataat tgtgaacaaa ttgaatcaat atttggatac
      361 ggaattgcta aaatcaaaac ttgggattct tttgcaactg aaatattatc tttacctaag
      421 atttttccat caacatttcc ataggtatct tttgccaagt cagaatattc atttgcggca
      481 gatattgctc gttcagcatc acgtgaacca attattatct catgtttttt acaccatctc
      541 aaggcaaatc ctttacccat acctccagta ccaccaataa ttccaatttt catgaattca
      601 atctaacaat cttattatta tctctatttg aaatttgaat caagccaaaa tgataatatt
      661 cctaagacac ggtcaagcag aaaataacac aaaacgtata cttgctggac gtactgaggg
      721 tgtaccttta actaaaattg gaatagaaca ggctgaacaa atcgggaaat atctaaaacc
      781 tattggcatt tctgctatat attcaagtcc aattgagcgt gcaaataata ccgcaaaaat
      841 tgttgcagag agtaactcaa ttgactacaa attggatgaa agacttactg agttagacat
      901 gggaaaattt acacgtatgc cttatgacga aatttttgca aaacacggta atgttttttt
      961 aaaattttat agtgatgatc ctatcatatc agaacataat gtagaaacat ttcctcacgt
     1021 acagaaacgt gtaatggaca tgctagatta cactattaaa aaacatgatc aagaaaatgt
     1081 gatacttgtt actcatatgg atccaatcaa atcagttatt gccaaagtga tagacctaaa
     1141 accattatct ttgtttgaat taataattgc aaattgttcc ctgacaatca taaaacatca
     1201 tgatgagaaa ttatcgcttt cagcaattaa tacaatggat gccgatcgat ttagccaaga
     1261 ttggtgattg gaaatcttac ataactttaa atagaattta tagtccacgt ccaaataacc
     1321 gactatgaga acaatatggg caccaatcat cctaattgta ttatttgcta ctccaatgat
     1381 tcaactagta tttgcatctg aaactcctgg ggaatatgtt aatagggcac aagaaatctg
     1441 gaacctcttt ttccgtatga tgacgatagc ctttgttgta ggtggagtaa ttgctggaac
     1501 tattgtatgg caagtttgga gatttaggga atcaaatccc aaagcaaaac ctactccata
     1561 tgaagataag ggtgacttgt aaaaatggga cattctaatt ggcctgaatg gatatatgtt
     1621 gcggttgtaa ttgcgctttt ggtttatgtt ggtgctgaag cgtggaatat tgaaaatctt
     1681 attgatcatg caccagaaga tgctgaaatt atcaaagtca ctgcacagca atggttctgg
     1741 agttttgaac atgcagatgg tacaaaagaa ataggcgaac ttcatttgaa aaaggaccat
     1801 gcatacaaat ttgaaattca cagtaaagat gttaaccata atttcaacat accagaattt
     1861 cttgttctaa tggatgcagt tcctggaagg atcaacactg tttggttttc tcctaacgag
     1921 tcaggcgagt ttgatataca gtgtagggaa tattgcggtc tgattcatta taacatgcgt
     1981 gctaaactat ttgtggaggg atcataatga cgccacattt aattctgtat gataaactaa
     2041 atcaaaacat ggaggataga aattggtact aacattagca aaaccacgtc caatatggca
     2101 aataatgttc tcaactcatc atacagatgt tggattaatg tatctaattt tctcaattat
     2161 ggcgctgttt ataggaggcg caatggctat tgctattcgt gtcgagttgt ttgcacctgg
     2221 tgcacagatt attgcagatg caatgacttt caacagatta ttcacatccc atggaactac
     2281 catgatcttc ttgttcttac tcccatctgc atcagctgtt ggtaattatc ttgtaccaat
     2341 tatggtgaga tacaaagaca tggc
//
LOCUS       DASCPJ010000049         4630 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000831011, whole genome shotgun sequence.
ACCESSION   DASCPJ010000049 DASCPJ010000000
VERSION     DASCPJ010000049.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000831011"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            156..455
                     /locus_tag="QGF01_02325"
     CDS             156..455
                     /locus_tag="QGF01_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19767.1"
                     /translation="MDSKELQGFISDVREDLNNFDELFDYYKFIDDHNIRKIVFNPDN
                     DENFFEGSVIAVLEQRYREFFRSRFNADMDDLVRIDVLEIIRSYLPAIHEKIDQE"
     gene            complement(458..1087)
                     /locus_tag="QGF01_02330"
     CDS             complement(458..1087)
                     /locus_tag="QGF01_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase"
                     /protein_id="HJN19768.1"
                     /translation="MGKKTREHRRERREDFASKRTHQKRKSNLLAAGILGIIAVIVGF
                     SIYTFIEMDSSDAMGLPEGAGKLGDEHEHASLLVRIFGDQFDFSTSTYQIKNSWIHFE
                     ESDGKTIHRHSSGVTLEYLFDSLNLTVGKVSTDGILKDCFAFPESDGRKFCTNEDYSL
                     KFYINHQAVSEIKDYVIEDGDRILISYGNETEEQINEQLMELDAQPIND"
     gene            complement(1157..1411)
                     /locus_tag="QGF01_02335"
     CDS             complement(1157..1411)
                     /locus_tag="QGF01_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19769.1"
                     /translation="MIFRIETIIGDRHESPDSLTNEQVHQWLEKIQKHDILKVETEDD
                     YWEDVPDELFELLKTNIDAEKYDYTMAKGHLWLNVDIPIE"
     gene            1464..1874
                     /locus_tag="QGF01_02340"
     CDS             1464..1874
                     /locus_tag="QGF01_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HJN19770.1"
                     /translation="MPSCIFCDMIRGNGSYHEIYQDEDKLAILDKYPIDIGHSLVIPK
                     KPYEKLTDMSIDEVSELFSKIPKIANAIIKATNADGFSIAQNNGKAAKQIIPHVHVHI
                     IPRYNSTGTEWTKRKILNEDELDELAKKIRVHLK"
     gene            complement(1878..3158)
                     /gene="prf1"
                     /locus_tag="QGF01_02345"
     CDS             complement(1878..3158)
                     /gene="prf1"
                     /locus_tag="QGF01_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HJN19771.1"
                     /translation="MGKIKVEKYDSVKLYKIRKTIEELSKIAGRGTELITVYIPKGKQ
                     LHEVITILREEQGTADNIKSDLTRTHVVDSLSKVVQRLKLYKKTPERGLAIFCGALPR
                     EVGGPPGSEVVKAFEIDPPKDMQTFLYRCDDHFHVDILKNMLKDENMIGFLAVDAKDA
                     GWGLLHGDKIEVLKETSSGVAGKHRQGGQSAKRFQKLREMELTYYFNRLATITREYFI
                     DIYPIKGLVISGPGPTKEEFINNNYLEYRLQNMIIATIDASYSGSEGIREAFAKSSDI
                     LSNFRMVEEKQIVEKLFNEINNNTGLGIYGLDDIINHLKNNIVDKIIITDNTELYRIE
                     SKCKKCEHIQEKIVERIKVISTKTELQNSSCLSCQSTDINVFDQDIIDYIAILALKTG
                     TTVEVISGVSEHGTMLASIGKIGAILRYNPNYSN"
     gene            3304..3516
                     /locus_tag="QGF01_02350"
     CDS             3304..3516
                     /locus_tag="QGF01_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HJN19772.1"
                     /translation="MNTKQFVLSTRTVPFSALIILTSILCLGLFFVGFDQGHFFSIVQ
                     GEDAFIEQFLHEFTHDMRHAAGFPCH"
     gene            complement(3524..3871)
                     /locus_tag="QGF01_02355"
     CDS             complement(3524..3871)
                     /locus_tag="QGF01_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HJN19773.1"
                     /translation="MKGKLPKPSILELNEYDVKQRIIEALTNTCSHAILFSIVTEAKN
                     AVQISQELQLSLSAVYKTLANLEKLTLVTIEDFEFSNEGKKTKRYRSKIKKASIEIGV
                     NDYEVNLYSVKTN"
     gene            complement(3877..4206)
                     /locus_tag="QGF01_02360"
     CDS             complement(3877..4206)
                     /locus_tag="QGF01_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HJN19774.1"
                     /translation="MQHKLTLPQLKKYDITQKVIEALADAESRAIIFTIIRKGKTAYD
                     LAETLKIPLSSVYKKLTDLENLTLIQVEKFMTTDSGRRLKIYRSRIKKADIIIKSKEP
                     EIQLSAN"
     gene            complement(4293..>4630)
                     /locus_tag="QGF01_02365"
     CDS             complement(4293..>4630)
                     /locus_tag="QGF01_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1297 domain-containing protein"
                     /protein_id="HJN19775.1"
                     /translation="PSFNVIGNSPLVLRESLLDEVYTMGENFVDAAKRVVSPGMNGPF
                     CIEGVYDENAKFTSFEFSARIVAGTNIFMNGSPYTTLIYNEPMSMGRRIAREIKQAEI
                     ENKISEITT"
BASE COUNT         1447 a          736 c          658 g         1789 t
ORIGIN      
        1 ccatagcctt gtacatagcg cgatgaggtg atctagctgt tccttccact acatttctac
       61 tagatatttc catttttaat acaataaagt aaaattacta taatttaaat tctaaaataa
      121 aaatttgata aactagtagt aattaaaaaa tatctatgga ttcaaaagaa ctccaaggct
      181 tcatttctga tgttagagag gatcttaata attttgatga gttatttgat tattacaagt
      241 tcatagatga ccataacatc agaaaaattg tttttaaccc agataacgat gagaattttt
      301 ttgaaggaag tgttatagca gttttggagc aaagatatcg tgaattcttt cgtagtagat
      361 ttaatgctga tatggatgat cttgttagaa ttgatgttct agaaattatt agaagttatc
      421 ttccagctat tcatgaaaaa attgatcaag aatgatacta gtcatttatg ggctgtgcgt
      481 caagctccat taactgttcg ttgatttgtt cttctgtttc gttaccatac gaaattaaaa
      541 ttctatcacc atcttcaata acataatctt taatttcact aacagcttga tggttaatgt
      601 agaattttaa tgaataatct tcatttgtac aaaactttct tccgtctgat tctggaaatg
      661 caaagcagtc tttgagaatt ccatcagtgg atactttacc aacagtaagg tttagactat
      721 caaataaata ttcaagtgta actccagatg aatgcctatg gattgtttta ccatcgcttt
      781 cttcaaaatg aatccaacta ttttttattt gatacgtact agtagaaaaa tcaaattgat
      841 cgccaaaaat tcttacaagt aatgacgcat gttcatgttc atcacctaat tttccagcac
      901 cttcaggtag ccccattgca tcgctagaat ccatttcgat aaatgtataa attgaaaatc
      961 ctacgataac tgcaattatt ccaagaattc cagctgcaag cagattagat ttccgttttt
     1021 gatgtgtacg cttagaggca aaatcttcac gcctttcacg tctatgttct cttgtttttt
     1081 tacccaatgc ttcagaaatt tatcatagcc ctatataaca tatgaaatta taatattata
     1141 cattcgattt agttttttat tcaatgggaa tatctacgtt aagccacaag tgtcctttag
     1201 ccattgtata gtcatatttt tcagcatcaa tattggtttt taataactca aaaagttcat
     1261 ccggtacatc ttcccaataa tcgtcttcag tttctacttt caaaatatca tgcttctgaa
     1321 ttttttctaa ccattgatga acctgttcat ttgttaatga atctggagat tcgtgtctgt
     1381 ctccaataat tgtttcaata cgaaatatca ttggattatt gtattggtgc taatatatta
     1441 atggaatttt ataggtagat gtaatgccat cttgtatttt ttgtgacatg atacgaggaa
     1501 acggttcgta tcatgaaatt tatcaagatg aagataaatt agctattttg gataaatatc
     1561 caattgatat tgggcattca ttggtaattc caaaaaaacc atatgaaaaa ttgacagata
     1621 tgagtattga tgaggtatca gaattatttt caaaaattcc aaaaattgct aatgctataa
     1681 ttaaggcaac aaatgctgat ggatttagta tagcacagaa taatgggaag gccgcaaaac
     1741 aaattatacc acatgttcat gttcacataa taccaagata caattcaact ggtacagaat
     1801 ggacaaaaag aaaaattttg aatgaagacg aattagatga attggctaaa aaaatacgag
     1861 ttcatctgaa ataattttta gttagaataa ttgggattat agcgtaaaat ggcacctatc
     1921 ttaccaatac ttgcaagcat ggtaccatgt tcagaaactc ctgaaatcac ttctacagtt
     1981 gttccagttt ttaatgcaag tattgcgata taatcaatta tgtcctgatc aaatacattg
     2041 atgtcagttg actgacatga taaacatgac gaattttgta attccgtttt ggttgaaatt
     2101 acttttattc gttcaacaat tttttcttga atatgttcac attttttaca ttttgactca
     2161 attctataaa gttcagtgtt atctgtaatt atgattttat ctacaatatt attttttagg
     2221 tgatttatta tatcatctag tccatatata ccaagaccag tattattatt tatttcatta
     2281 aataattttt caacaatttg tttttcttcc accattctga aattagaaag aatgtctgaa
     2341 gattttgcaa atgcttcacg tattccttct gaaccagaat aagatgcatc aattgttgca
     2401 ataatcatat tttgcaatct atattcaaga taattattgt tgatgaattc ttcttttgtc
     2461 ggaccaggtc cagaaattac taatcctttg attggataaa tatcaataaa atattctcta
     2521 gttatagttg ccaatctatt aaaataatat gttaattcca tttcccttag tttttggaat
     2581 ctttttgcag attgtcctcc ctgcctatgt tttccggcaa ctccagaact tgtttccttt
     2641 aaaacctcaa ttttatcacc atgtaataat ccccatccag catccttagc atcaactgct
     2701 aagaatccaa tcatattttc atcttttaac atatttttta gtatgtcaac atggaaatga
     2761 tcatcacatc tgtataggaa agtttgcatg tcttttggcg gatcaatctc aaatgctttt
     2821 accacttcac tgcctggcgg tccacccact tctcttggta atgcaccaca gaatattgct
     2881 aaacctcttt caggagtttt tttatataat tttaatcgtt gtactacttt tgataacgag
     2941 tcaacaacgt gcgttctagt aagatctgat tttatgttgt cagcagtacc ttgttcttct
     3001 cttaaaatag taattacttc atgtaattgt tttcctttag gaatatagac agtgattaac
     3061 tctgttccac gacctgctat ttttgatagt tcctcgattg tttttcttat tttgtacagt
     3121 ttgactgaat catatttttc aaccttgatt tttcccatat tctaccttgg gatttattaa
     3181 tataagataa atgattttaa tttttttatt ttattttaga ataatttgat gaaatatttc
     3241 agatatttga tgatctataa aataaataac ttaattaaag ctcacaaatc aattgattta
     3301 agaatgaata ctaaacaatt tgtactttct actagaactg ttccattttc tgctttaatc
     3361 atactcactt caattctttg tttaggatta ttttttgttg gatttgatca aggacatttt
     3421 tttagtattg tgcaaggtga agatgctttt attgaacaat ttttacatga attcactcat
     3481 gatatgagac atgccgcagg atttccatgt cattagataa aatttagttt gtttttacac
     3541 tataaagatt tacttcataa tcattgacac caatttctat actggctttt ttgattttac
     3601 ttctataccg tttagttttt tttccttcat tcgaaaattc gaagtcctct atagttacta
     3661 gtgttaattt ttccagattt gccagtgttt tataaactgc tgataatgat aattgtaatt
     3721 cttgagatat ttgcactgca ttttttgcct ctgtgacaat tgaaaataat attgcatgtg
     3781 aacatgtatt tgtcaaggct tctatgatac gctgtttcac atcatattca tttagttcaa
     3841 gtattgatgg ttttggaagt tttcctttca ttttaatcaa ttagcagata gctgtatttc
     3901 tggttccttg ctttttatta ttatatcggc ttttttaatt ctgctacgat aaatttttag
     3961 acgtcttcct gaatcggtag tcataaattt ttccacctga ataagcgtta aattttctag
     4021 gtctgtcaat tttttatata cactacttaa tggaattttt agcgtttcag ctaagtcata
     4081 cgcagtttta ccttttctga tgattgtaaa tataattgct cttgattcag catctgctaa
     4141 agcctcaatt actttttgtg ttatatcata tttttttaat tgcggtaatg tgagtttatg
     4201 ttgcattttt catattggtt atcatctata aaaaaatgtt tttatttatt ctaagaatat
     4261 agaataaaaa tcataatatg tatgaatgtg ttttatgttg ttatttcact aattttattt
     4321 tcgatttcag cttgtttaat ctcacgtgca atacgacgac ccatactcat aggttcgttg
     4381 tagattagtg tggtgtaagg tgaaccattc ataaaaatat ttgtacctgc tacaattcga
     4441 gcagaaaatt caaatgatgt aaatttagca ttttcatcat atactccttc gatacaaaat
     4501 ggaccattca tacctggaga aacaactctt tttgcagcat caacaaaatt ttcacccatc
     4561 gtatacactt catctaataa tgattctctc aaaactagtg ggctgtttcc aattacgtta
     4621 aaggatggaa
//
LOCUS       DASCPJ010000050         8855 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000861363, whole genome shotgun sequence.
ACCESSION   DASCPJ010000050 DASCPJ010000000
VERSION     DASCPJ010000050.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000861363"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            110..634
                     /locus_tag="QGF01_02370"
     CDS             110..634
                     /locus_tag="QGF01_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HJN19776.1"
                     /translation="MSLLIKDYKKIAKTRRQRGYQWEDTLVKRFNSLEGWKAFRLGSP
                     SVALPDVLVVNNKKSVIFTIEAKSGTGTTLFVPYDQIERCQRWTNNFEVYKTRKVILA
                     FKFLSKKRIGTGKYERRKLHEFYKIWGNKKKAVNCVCTYNGEMYESSNGERKELCLND
                     LPMPFKSKYQIITK"
     gene            677..1444
                     /locus_tag="QGF01_02375"
     CDS             677..1444
                     /locus_tag="QGF01_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19777.1"
                     /translation="MNIKGYFKKIQNKSDNKKSKSELDDKRDAPKEGLKASILANEME
                     ARIETTLNFRDKNKDPHEYLKRYIQEPEYRDWFKRNFPNRTIYEAVGLTAMDYLEVKR
                     SMDNESEQGSEVHTKQKNLKKESSFKPIVKEDPKINFNDFIDERMLVSHNAFGDKEMK
                     IKIVEVSDEIAPLVTKFGDRVKVNKVLVTIKHLKTQKVEEAEFDIESIERELMEKRHY
                     TSTNRWVPTKEIKNGYVTNTRHTILITDAASLDYIIF"
     gene            1516..1833
                     /locus_tag="QGF01_02380"
     CDS             1516..1833
                     /locus_tag="QGF01_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19778.1"
                     /translation="MITKDLKIAGQSFHLTLTDHTITQVNSLKSLYSTAYEDPESFEQ
                     VSAEISSTIKQISSAVEPEINDENLDELIQEIIKIVDAKAAQIQKQSEEKIKKPSTKK
                     SKK"
     gene            complement(2046..2432)
                     /locus_tag="QGF01_02385"
     CDS             complement(2046..2432)
                     /locus_tag="QGF01_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19779.1"
                     /translation="MKIVLEDFSEYLKGVMRQILDSYNIIIQLDDEPSDLAIIKKELS
                     KIRGQLTVLKNKLKSKEYQSDDLVTLYKVSLYYTETYDFSREINLLSQIYYNDPLRLK
                     NLRLLIIESLNDKKLIEKFQEIFNRL"
     gene            2535..3866
                     /locus_tag="QGF01_02390"
     CDS             2535..3866
                     /locus_tag="QGF01_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HJN19780.1"
                     /translation="MNSYLKCVNQKCGKEYPILSTDFKCSCGNLLDVKYKNSPSSNLK
                     EVFYKRRNPEGSIFNESGVWRFRELLNFCEIDTEDLEQCSKNLVSLDGSEGRQSKPYH
                     MSKVASFVGIDNERLMLQPEGYNPSGSFKDNGMSAAVTHAKMVNAKKIICASTGNTSA
                     SAGMFAANENMECDVYIPEGEISPGKLSQAYQFGTQMIHVKGTFDDAFVKSLNASKES
                     GSYTVNSINPFRIEGQKTIPYRALEFLNWEVPDWIVYPGGALGNTSSCGKCIMELYEW
                     GWIKKIPRIAVINAEGANTLDVLYNGKFEETELRWNKGDPNVELIDQYYSKMNDDGIR
                     PQTKATAIQIGKPANILKGLRALEFTDGVVTSVTDNEMLDGMSIVGLNGFDCEMASGA
                     VPAGVKKLMGEEIIKKDDTVIGILTGRQKEPLLPIEYHNDISNKFARPPKE"
     gene            3998..5242
                     /locus_tag="QGF01_02395"
     CDS             3998..5242
                     /locus_tag="QGF01_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HJN19781.1"
                     /translation="MQLEYEIAALIAMIGLSGYFSGLEVALVSIRISKIEQMIKDKVK
                     GATSLHKIKSNPSRMMSSVNLGNNLASIAATALATDISLKLFGDEGLAIAIGIMTFLI
                     LVFSEITPKTYCNANPTKVAIKNSRILLVFTYALFPIVWFFEIITRGMMKLTGSSDMP
                     PGLTEDEIKGIVDQGLKDKAIEKQESELVHGALNFDDIVIRSVMTPRTKMFTLNSKTV
                     LFEALPQINQSGFSRIPIHGDNKDEIVGIIHVRDVLKALENDQKTHTLEQISRNPIFV
                     SQEKMVSDLLKEMQGRQTHMAIVVDEFGGVEGCVTLEDLLEEIVGEIRDETDTNQQVF
                     QNDGPDAIITNGDIEIDTINEIFKTYLSAGDDYSRLNGLLHEKLHDIPKEGDKLEINS
                     LRIIVEKVSKNKPERIRIEKIK"
     gene            complement(5243..6292)
                     /locus_tag="QGF01_02400"
     CDS             complement(5243..6292)
                     /locus_tag="QGF01_02400"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HJN19782.1"
                     /translation="MKNVFVNGYGSIGSRIAEFIKDDSEINVIGVGKHSPDEKVDLAI
                     KNGFSVYVPQKKIDNFKNYNISGSIESVLSDCDLVIDASPEGHGFINKKNLYEPNNIM
                     AIYQGGETIYGDDAVSDLIFNSRVNYKKVLQKKHVMQGSCNVTGMGKILEPLRNKYGS
                     NLIRFDVTLVRRWADLDQSDKQLVDTIEMSQHPHHGNDVKSYFGKDAPLFVRAIKVPT
                     RQMHLHIMDIRFSGTAPNVDEIHSLFKDESGVVVLWTATNTKDVRDFANTMKFSFKDT
                     NMVHIHANMSVAINDTVQIMYSDDQTGIVIPENHMLMQAMLFERSYEDALSHTNELFH
                     MGDKKKLLEEHFRKN"
     gene            6448..7842
                     /locus_tag="QGF01_02405"
     CDS             6448..7842
                     /locus_tag="QGF01_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HJN19783.1"
                     /translation="MAAGIDDIALYIPNLYLEASDFAEARGLDPAKLQRGLGVEQMAI
                     VDTNQDPACLAANACLNLMQKNKLSPEKIGRLYVATESAFDESKAMNSYVIGMLEQVY
                     GNESFGHCGGIECKFACVSGSYALYDNTNWIRAGEAEGQNAIVVVSDIAKYDLGSSGE
                     VTQGAGAIAMLLNDNPRLLAFDPKVTSTSIKNEYDFYRPFGKETPIVHGQYSNLLYLI
                     QVKNALADYKEKILKTGLVTLKDDETILDHIDYLNMHLPYSNMGKKALAYLVLHEWRS
                     LPRWKDIIAEIGMEEPVPKDPRGTIESVLADAEFMAKDHEFTKLFTKTEKYAEVYESK
                     LASSLIASKMVGNLYTASLYLGFRSSLEFEYQKGVDLNGKRIGFGSYGSGASAMVFSG
                     LVQSEYEEVVKDMNLEAEIGVRTKLTLEEYEELHENKRNTENNIHSAKKEFILTDVKT
                     SPESRGERYYEYIE"
     gene            7848..8399
                     /locus_tag="QGF01_02410"
     CDS             7848..8399
                     /locus_tag="QGF01_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19784.1"
                     /translation="MSENSSIKDTLYSKINAIGQETIHKLLLEKKYSELFELTYTKAL
                     QSINTKIGEIEKMGILAESFTHYLFTEMLIPSQRKISYENIELDMIIPNLVELKKNSD
                     NSILIFFVKSSNNNIIKQRIQTVKKIQKTDKNIWVISKDKINISQKVYKIEKESFSRF
                     LDDIQNFTELKNMNKLNIFKSKV"
     gene            complement(8373..>8855)
                     /gene="bioD"
                     /locus_tag="QGF01_02415"
     CDS             complement(8373..>8855)
                     /gene="bioD"
                     /locus_tag="QGF01_02415"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HJN19785.1"
                     /translation="PYFFEIPTSPYDACKILAQKINLKKIIDAYHKLLTSHDLLIVEG
                     IGGLMTPITQNYFVSNLISELNIDTIIVTGSKLGTVNHTLLTYEHCKQMQLKLKGFVI
                     NQTEHDGYKLSNLKQQIIELTNQIVYCAIPYHKNFELDMYVDNFPNFVNVSNFGFEDI
                     "
BASE COUNT         3374 a         1217 c         1460 g         2804 t
ORIGIN      
        1 gtaatttctg aaatttagta tcattttcaa tgtattgata gcgatcatta acacattgtt
       61 gataatataa ttaccagcta ttctatatcc ttcaaaaaca caggctagta tgtcactttt
      121 gattaaagat tataaaaaaa ttgcaaaaac tagaaggcag agaggctatc aatgggaaga
      181 cacattggtg aaaagattca actcgcttga aggatggaaa gcatttaggc taggatctcc
      241 tagtgttgca ttgccagatg ttttagttgt taataataaa aaaagtgtaa ttttcacaat
      301 agaggcaaaa tctggaactg gtactacatt gtttgttcca tatgatcaaa tagaaagatg
      361 tcagagatgg acaaataatt ttgaggttta taaaacaaga aaggttattc tggcattcaa
      421 atttctgtca aaaaaaagaa taggcacggg taaatatgaa agaagaaaat tacatgaatt
      481 ttacaaaata tggggtaata agaaaaaggc tgttaactgt gtctgtacat ataatggtga
      541 gatgtacgaa tcatctaatg gtgaacgaaa agaattatgt cttaatgatt tacctatgcc
      601 atttaaatcc aaatatcaaa taataacaaa ataaccaaat gtaatcattt tttaataatc
      661 aaatacaaca tatattatga atatcaaggg ttattttaaa aaaattcaaa ataaatctga
      721 taataaaaaa tcaaaatctg aactagatga taaaagagat gccccaaaag aaggattaaa
      781 ggcatcaatt cttgctaatg aaatggaagc ccgtattgaa accactttaa attttagaga
      841 taaaaacaag gatccgcatg agtatttgaa aaggtatatt caggaaccag aatacagaga
      901 ttggtttaag cgtaattttc ctaatcgaac aatctatgaa gcagttgggt taacagctat
      961 ggattatctt gaagtcaaaa gaagtatgga taatgaatca gaacaagggt ctgaagttca
     1021 tacaaaacaa aagaatctaa aaaaagaatc cagctttaag cctattgtta aagaggaccc
     1081 taaaatcaat tttaatgatt tcattgatga acgcatgtta gttagtcaca atgcatttgg
     1141 tgataaagaa atgaaaataa aaatagttga agtttctgat gaaatagctc cattagtaac
     1201 aaaatttggt gatcgggtaa aagtaaacaa ggtattagtt actataaaac acctaaaaac
     1261 tcaaaaagta gaagaagccg aatttgatat tgaatcaatt gaaagagaat taatggaaaa
     1321 acgacattac acatcaacaa accgatgggt tcctactaaa gaaatcaaaa atggatatgt
     1381 aactaataca agacacacaa ttctaatcac tgatgcggca tcattagatt atatcatatt
     1441 ttgaatcaat aatctctaat tatattttat aatcaagaag aaaaagtata agaagctcac
     1501 aaaaaaagct ctgatatgat tacaaaagat ctgaaaatcg caggacagag ttttcatctt
     1561 acgttgacag accatacgat aacccaagta aatagcttaa aatcacttta tagcactgca
     1621 tatgaagatc cagaaagttt tgaacaagtt agcgctgaaa tttcatctac cattaaacaa
     1681 atttctagtg cagtagaacc agaaataaat gatgagaatt tggatgaatt aatacaagaa
     1741 attatcaaaa tagttgacgc taaagctgct cagattcaaa agcagtcaga agaaaaaatt
     1801 aaaaaaccat caactaaaaa atcaaaaaaa taaaatattt gatgttttga ttaatccaaa
     1861 tttagaatag aagtaaacgt gacgttttag ttatatttaa ccagtacaat atactatatg
     1921 gtaaaacact gtgaatgtgg aatatgtggg cattttactg aagatgactg tataaaaaat
     1981 aaatgtaaat gttgtagtaa ttttcatcta agatctggct aagtgataat aaacatcctt
     2041 taacgttata acctattaaa aatctcttga aatttctcaa tcaatttttt atcatttaat
     2101 gattcaatta ttaataatct taaatttttt aatcgtaaag gatcattata ataaatctgt
     2161 gataataaat taatttctct actaaaatca taagtttctg tataatataa tgaaacttta
     2221 tagagtgtaa ctaaatcatc agactgatat tccttacttt ttaatttgtt ttttaataca
     2281 gtcaactgtc ctcttatttt tgataattct ttctttataa ttgccaaatc acttggttca
     2341 tcatctaatt gaatgattat gttatacgaa tctaaaatct gccgcataac tcccttcaga
     2401 tattcagaaa aatcctccaa aacaattttc actgtacata acccttcaaa aagtttagta
     2461 atttggatca aatttgcata aatatcttat ttcttatctt taaattactc aaaaactaaa
     2521 ttagcacatt gtaaatgaac tcatatttaa aatgcgttaa tcagaaatgt ggaaaagaat
     2581 atcctatttt atccacagat ttcaaatgtt cttgtggtaa tcttttagat gttaaatata
     2641 aaaatagccc atcatccaat ctaaaagaag ttttttacaa aagaagaaac ccagaaggta
     2701 gtatattcaa tgaaagtgga gtatggagat ttcgtgaact tttaaatttt tgtgaaatag
     2761 atactgaaga tttagagcaa tgttcaaaaa atcttgtgtc attagatgga tctgaaggca
     2821 gacagtcaaa gccatatcac atgtcaaaag ttgcttcatt tgttggaatt gataatgaga
     2881 ggttgatgtt acagcctgag ggatataatc caagtggctc attcaaagac aatggtatgt
     2941 cagctgctgt tacacatgca aagatggtta atgcaaaaaa aataatctgt gcatcaacag
     3001 gtaatacctc tgcttctgct ggtatgtttg ccgccaatga aaacatggag tgtgacgtgt
     3061 atattccaga aggagaaatt tcaccaggta aacttagtca agcgtatcaa tttgggacac
     3121 aaatgataca tgtaaaaggt acttttgacg acgcttttgt aaaatcactt aatgcgtcaa
     3181 aagaatcagg aagttataca gtaaattcaa ttaatccgtt tcgtattgaa ggtcaaaaaa
     3241 ctattccata tcgtgcacta gaatttttga attgggaagt accagattgg atagtttatc
     3301 ctggtggtgc attgggtaat acgtcaagtt gtggaaaatg tattatggaa ctttatgaat
     3361 gggggtggat aaaaaaaatt ccgcgtattg ctgttattaa tgcagaaggt gcgaatacat
     3421 tggatgtttt atacaatgga aaatttgaag aaactgaatt aagatggaat aaaggagatc
     3481 cgaatgttga attaattgat caatattatt caaaaatgaa tgatgatgga attaggccac
     3541 aaacaaaagc aacagcaata caaattggta agcctgctaa tattttgaaa ggattacgtg
     3601 cattagaatt cactgatggt gttgttacat ctgtaacaga taatgaaatg ttggatggta
     3661 tgtcaattgt tggtttaaat ggattcgact gtgaaatggc ttctggagct gttcctgcag
     3721 gtgtaaaaaa actaatgggt gaagaaataa tcaaaaaaga tgatacagtt attggtattt
     3781 taactgggag gcagaaagaa ccattacttc caatagaata ccataatgat atatcaaata
     3841 aatttgcacg cccacctaaa gaataaattt gagcgtatac tctctatcaa aaaatgtgaa
     3901 cccttaaaag atcgagtttg tatctggtat taacaagaaa tgtggtgttt tctaagttaa
     3961 tgtgcagtat tttaaaattt gaagagtggg gtaatctatg caattagaat atgaaattgc
     4021 agcactaatt gcaatgatag gtctttctgg atatttcagt ggtttagaag ttgcattagt
     4081 tagtattagg atttctaaaa ttgaacaaat gattaaagat aaagtcaaag gtgctacatc
     4141 acttcataag ataaaatcta acccaagccg aatgatgtct agtgttaact taggaaataa
     4201 tttggctagt atagcggcaa ctgctttagc aactgatatc tcattaaaat tatttggtga
     4261 tgaaggatta gcaattgcaa tagggattat gacattttta attctagtat ttagtgaaat
     4321 cactccaaaa acatattgta atgcaaatcc aacaaaagtt gcaataaaaa atagtagaat
     4381 actattggta tttacctatg cattatttcc aattgtgtgg ttttttgaaa taatcacaag
     4441 aggtatgatg aaactcacag gtagtagtga tatgccgcct ggattaacag aagatgaaat
     4501 taaaggaatt gttgatcaag gcttaaaaga taaagcaatt gaaaaacagg aaagtgaact
     4561 tgttcatggt gcattgaatt ttgatgatat tgtcattaga tctgtaatga caccaaggac
     4621 aaagatgttt acattaaatt caaaaacagt attatttgaa gcattaccac agattaatca
     4681 aagtgggttt tctagaatac caattcatgg tgataataaa gatgaaattg ttggaattat
     4741 acatgttaga gatgttctga aggcgttaga aaacgatcaa aaaacacaca cattagaaca
     4801 aatttctaga aatccaattt ttgtttcaca agaaaaaatg gttagtgatc ttcttaaaga
     4861 aatgcaaggt aggcaaacgc atatggcaat tgttgttgat gagtttggtg gcgtagaagg
     4921 ttgtgtcacg ttagaagatc ttttagaaga aattgtcggt gaaattagag atgaaacaga
     4981 tacaaaccaa caagtattcc aaaatgatgg tccggatgca ataattacaa acggtgatat
     5041 tgagatagat acaataaacg aaatttttaa aacatattta tcagccgggg atgactattc
     5101 acggttgaat ggcctattac acgaaaaact acatgatata ccaaaagaag gtgataaatt
     5161 agagataaac tcgttacgaa tcatagtaga aaaagtttca aaaaataaac ctgaaagaat
     5221 aagaattgaa aaaataaagt aattaatttt tcctaaaatg ctcttctaat agttttttct
     5281 tatctcccat atgaaacaat tcatttgtgt ggctaagtgc atcttcatat gatctttcaa
     5341 aaagcattgc ctgcattaac atatgatttt ctggtatcac aattccagtt tgatcatccg
     5401 aatacatgat ttgtactgtg tcattaatcg cgacactcat gtttgcatga atatgtacca
     5461 tatttgtatc cttaaaacta aacttcatag tatttgcaaa atctctaaca tcctttgtat
     5521 ttgttgcagt ccataacact acaacaccac tttcatcttt gaacaaacta tgaatttcat
     5581 ctacattcgg cgctgtaccg ctaaatctta tatccattat atgaagatgc atttgacgtg
     5641 taggaacttt aattgcacgt acaaataatg gtgcatcctt tccaaaatat gatttaacat
     5701 cattaccatg atgaggatgc tgactcattt ctatggtatc cactaattgt ttatcagatt
     5761 gatcaagatc agcccatcgt cttactaacg taacatcaaa acggattaaa tttgaaccgt
     5821 atttattacg tagtggctct aaaatttttc ccattcctgt aacattacaa cttccctgca
     5881 tcacatgttt tttctgaagt acttttttat aatttacacg tgaattgaaa ataagatctg
     5941 aaactgcatc atcaccataa attgtttcac caccttgata aatcgccata atattatttg
     6001 gttcgtaaag atttttttta ttaataaatc catgcccctc cggagatgcg tctattacta
     6061 aatcacaatc agacagaaca gattcaattg aacctgaaat attataattt ttaaaattat
     6121 caattttttt ctgtggaaca tatacagaaa atccattttt tattgcaaga tcaacttttt
     6181 catctggaga atgttttccc acaccaatta catttatctc cgaatcgtct ttgataaatt
     6241 cagcaattct acttccaatg gatccatatc cattgacaaa tacattcttc atgtatattt
     6301 ttcacataat tcatttattt acatactggt atcttttctg tacgcatgaa ttagattttt
     6361 ctgttgtgat gtagcgatct aaaaattgta aattatatag aaaaattcac ttggaaatgt
     6421 atatatgcta agtttcgaag gcaagctatg gcagcaggaa tagacgatat tgcactatat
     6481 attccaaatt tgtatctaga agcatcagat tttgctgaag caagaggctt agatccagca
     6541 aaattacaac gtggtctagg tgtagagcaa atggctattg tagatactaa ccaggatcca
     6601 gcatgtcttg cagctaatgc ttgtcttaat cttatgcaaa aaaataaact gtcaccagaa
     6661 aaaattggta gattatacgt tgcaacagaa tctgcttttg atgaatcaaa agcaatgaat
     6721 tcttacgtta ttggaatgct agaacaggta tatggtaatg aatcatttgg acattgtggt
     6781 ggaattgaat gtaaatttgc ctgtgtcagt ggttcatatg cattatatga taacacaaat
     6841 tggataagag ctggtgaagc tgaaggtcaa aatgctatag ttgtagtttc agatattgca
     6901 aaatacgatc ttggttccag tggtgaagta acacaaggtg caggtgcaat tgctatgtta
     6961 cttaatgaca atcctagatt attagcattt gatccaaaag ttacatcaac atctatcaaa
     7021 aatgaatatg atttttacag gccatttggt aaagaaacac caattgttca tggacaatat
     7081 tctaacctct tgtatctaat tcaagttaaa aatgcactag cagattataa agaaaaaatt
     7141 ctaaaaacag gacttgttac gctaaaagat gatgaaacaa ttcttgatca tattgattac
     7201 ctcaatatgc atctcccata tagtaatatg ggtaaaaagg cacttgctta tcttgtacta
     7261 catgaatgga gaagtttacc aagatggaaa gacataattg cagaaattgg tatggaagaa
     7321 ccagtaccta aagacccacg tggaactata gaatcagttc ttgctgatgc agaatttatg
     7381 gcaaaagatc atgaatttac aaaactattt acaaaaacag aaaaatatgc tgaagtgtac
     7441 gaatcaaaac ttgctagttc attaattgct tcgaaaatgg ttggaaatct ttacacagca
     7501 tccttgtatc ttggatttag aagtagttta gaatttgaat atcaaaaagg agttgatttg
     7561 aatgggaaac gtattggatt tggttcttac ggaagtgggg ccagtgcaat ggtgtttagt
     7621 ggtttagtac aatcagaata cgaagaagtt gttaaagata tgaatcttga agctgaaatt
     7681 ggtgttagaa ctaaactcac attagaagaa tatgaagaat tacacgaaaa taaacgtaat
     7741 acagaaaata atattcattc agctaagaaa gaatttatcc ttacagatgt aaaaacatct
     7801 cccgaaagta gaggagagcg ttattacgaa tatattgaat aatacaaatg agtgaaaatt
     7861 catctatcaa agatacacta tattcaaaaa tcaatgctat tgggcaagaa acaattcata
     7921 aattactttt agagaaaaaa tattcagaat tatttgaatt aacatataca aaagcattac
     7981 aatccattaa tacaaaaatt ggtgagatag aaaaaatggg aatattagca gaaagtttca
     8041 cccattattt attcacagaa atgttaattc ctagtcaaag aaaaatttct tatgaaaata
     8101 tagaattaga tatgattatt ccaaatttag ttgaattaaa aaaaaattct gataattcaa
     8161 ttttaatatt ttttgttaaa tcatcaaata ataatataat aaaacaaaga atacaaacag
     8221 taaaaaaaat tcaaaaaact gataaaaata tttgggtaat ttctaaagac aaaatcaata
     8281 tctcacaaaa ggtttacaaa attgaaaaag aatcattttc tagattttta gacgatatac
     8341 aaaattttac agaattaaaa aacatgaata aattaaatat cttcaaatcc aaagtttgaa
     8401 acattaacaa aatttggaaa attatctaca tacatatcta attcaaaatt tttatggtaa
     8461 ggaattgcgc aatacacaat ttggttagta agttcaataa tttgttgttt taaattagac
     8521 aatttgtaac cgtcatgttc tgtttgattg atcacaaatc cttttaattt taattgcatt
     8581 tgtttacaat gttcatatgt tagtaatgtg tgattaacag tgccaagttt agatcctgta
     8641 acaattattg tatcaatatt aagttcagaa ataagatttg aaacaaaata attttgagtt
     8701 ataggggtca ttaatccccc aattccttca acaataagca aatcatgaga agtgagcagt
     8761 ttatgatatg catcaattat tttcttaagg ttgattttct gagcaaggat tttacatgca
     8821 tcgtatgggg aagttggaat ttcaaagaaa tatgg
//
LOCUS       DASCPJ010000051         2178 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000864049, whole genome shotgun sequence.
ACCESSION   DASCPJ010000051 DASCPJ010000000
VERSION     DASCPJ010000051.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000864049"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="QGF01_02420"
     CDS             <1..599
                     /locus_tag="QGF01_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN19786.1"
                     /translation="CGAGTYIFEDADTKKFVPMTRFCDLQGMLELFEDKSEQIRSGAN
                     KYFTMLEVVRKLKGFIDKDKQPAGLDLAKMFSTILMKRSFDAVGGFHVKGLFLGMMHF
                     QDKYNEDLERLQRCDIHYTTPDLRVIPFCAFNVIPEWYRDRIQKKYSITVEEWEEQQG
                     EKLEDGLYRGLMRRGAGDELAAGCAKSQMFHDAQQATM"
     gene            complement(646..>2178)
                     /locus_tag="QGF01_02425"
     CDS             complement(646..>2178)
                     /locus_tag="QGF01_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19787.1"
                     /translation="IILGSFFLVLPFFRNDKKFIVYATVIFIFSMSIVLSQLDPASFS
                     ASILSIDKDRGGVPGYDMLPGPLGVFMAGVLLFAFICAVIQKIKQRFRTSYGLIILGS
                     LVAIGFSAASIIGKLPSFRYQTAANPFLVTTDPLTESIAEHAFSSLDISFFYHSILMI
                     FAAIGIWLIFQNRINKSVHIKNEMAAFALITGIIGLYFSSAFVRLELFAAISMIILAS
                     IGISILISKIFAAQNTAKSITKISFVACVVILLVIPVGLPLNDNWVTSTSIAPAILYG
                     GSYLLAPTNDWPDAMLWLKTKTPEDAKILSWWDYGYWIETLGDRTTYADNATLDTQII
                     KKTAQMFYSSPDNAWKELVAIDADYVLIWVTAERVVSSSDIPMYLLGVGGGDETKRFW
                     FAKVADAPLEKYFFDDLVSGPNAFWHNTFLGKMIPFSPALYLDPVHEKNYKQYQPNTV
                     GVYVKDYKFSNNPIDALDEPLQLVYTSPSFDRTDDGEISGVIIYKVNKDYKPSFEYDI
                     LN"
BASE COUNT          770 a          373 c          370 g          665 t
ORIGIN      
        1 catgtggtgc aggtacatac atctttgaag atgcagatac aaagaaattt gttccaatga
       61 cacgattttg tgacctacaa ggaatgcttg agttgtttga agataaatca gaacaaattc
      121 gttctggtgc taacaagtac tttacaatgc ttgaagtggt aagaaaacta aagggtttca
      181 ttgataagga caagcagcct gcaggtctag atttagctaa aatgtttagc actattctca
      241 tgaaaaggtc gtttgatgca gttggaggtt tccatgtcaa agggttattc ttgggtatga
      301 tgcacttcca ggacaagtat aatgaagatt tggaaagact tcaaagatgt gatatacatt
      361 acacaacacc ggatcttaga gttattccat tctgtgcatt taatgtaatt ccagaatggt
      421 accgtgacag aattcaaaag aaatattcca ttacagtcga agaatgggaa gaacaacaag
      481 gtgaaaaact tgaagatggt ctttacagag gtcttatgag aaggggtgct ggtgatgagc
      541 tcgcagcagg ttgtgccaag agccaaatgt tccatgatgc acaacaagca acaatgtaat
      601 taaacggaaa aattatttct tatgaaatat acaaataact tttaattaat ttaatatatc
      661 atattcaaaa ctaggtttat agtccttgtt tactttgtaa ataatcacac cactaatttc
      721 accatcatca gttctatcaa atgatggtga agtgtatact aattgtaacg gttcatccag
      781 cgcatctatt ggattgttag aaaatttata atcttttaca tatactccta cagtattagg
      841 ttgatattgt ttgtagttct tttcgtgcac tggatcaaga tataatgctg gagaaaatgg
      901 aatcattttt cccaaaaatg tgttatgcca aaaagcatta ggtccactaa ctaaatcatc
      961 aaaaaaatat ttttctaaag gtgcgtctgc aacttttgca aaccagaatc ttttagtttc
     1021 atcaccgcca ccgacaccca ataaatacat aggaatgtca ctgctactca caactctttc
     1081 agcagtaacc cagatcaaaa cataatcagc atcaattgct actaattctt tccatgcatt
     1141 atccggagaa ctgtaaaaca tttgtgcagt ttttttgata atctgcgtgt ctaatgtagc
     1201 attatctgca taggtagttc gatcaccaag tgtttcgatc caatatccgt agtcccacca
     1261 tgaaagaatt tttgcatctt ctggagtctt ggtcttcaac cataacattg catcaggcca
     1321 atcatttgtt ggtgccagaa gataactacc accataaaga attgcaggtg caatacttgt
     1381 agatgttacc caattatcat tcagaggtaa tcccacaggt atgactaaaa gtatgacaac
     1441 acatgctaca aacgaaattt ttgttataga tttagcagta ttttgtgcag caaatatttt
     1501 tgatataaga attgatattc caattgatgc tagtattatc atagaaatag ctgcaaataa
     1561 ttctaatcta acaaatgcag agctaaaata aagaccaatt attcctgtta ttaatgcaaa
     1621 tgcagccatt tcgtttttta tatgaacaga tttattgatt ctgttttgaa aaattaacca
     1681 tattcctatc gcggcaaata tcattaaaat tgaatgataa aaaaatgata tatctaaaga
     1741 tgaaaaagcg tgttcagcga ttgactccgt aagaggatca gttgttacca aaaatggatt
     1801 tgctgcagtt tgataacgaa aactaggtaa tttaccaata attgatgctg ctgaaaatcc
     1861 tatagcaact aaacttccta aaataattaa accgtaacta gtcctgaaac gttgttttat
     1921 tttttgtatc acagcacaaa taaaagcaaa tagtaaaaca ccagccataa aaactccaag
     1981 gggacctggt agcatatcgt aaccaggtac accgccacga tccttatcaa tactcaaaat
     2041 actagcagaa aaagatgctg gatcgagttg tgataataca atactcatgc tgaaaataaa
     2101 tatcactgtg gcataaacaa tgaatttttt atcatttcta aaaaatggta ataccaaaaa
     2161 aaacgatccc aagataat
//
LOCUS       DASCPJ010000052         2429 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000868634, whole genome shotgun sequence.
ACCESSION   DASCPJ010000052 DASCPJ010000000
VERSION     DASCPJ010000052.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000868634"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="QGF01_02430"
     CDS             complement(<1..823)
                     /locus_tag="QGF01_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HJN19788.1"
                     /translation="MNERRIRIGIDVGGTFTKAVAIDIVTGNIISLSTVPTTHSSENG
                     VSDGIVLALSNILKNSDIKLNEIEIIAHSTTQAINSLLESDTAKVGIIAMGVGPEKKD
                     VIKRTNLQDAKINSQNDIKTSHRFLDTSHLITESEVSQAIQELKNDGAKVIVATEAFG
                     VDDPSNEMFVMENATKSGIPSTSSHEISGIYGLEIRTLTAAINASVLPKTVEVAGFVE
                     SAIRKAGVTAPLMIMKGDGGVTSMETFRTKPILTILSGPAASVAGALLYLKVTNGI"
     gene            complement(816..>2429)
                     /locus_tag="QGF01_02435"
     CDS             complement(816..>2429)
                     /locus_tag="QGF01_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HJN19789.1"
                     /translation="GTLISFIQRDLLGTDVLTTAGIDNSNLYNIIRDLYGELLIELDN
                     GNYDKAEEIGIEAYLENFEYLEPEIEIADAELLYQLEWDMREELRAMIKNQESPETIR
                     AFLVDSIIPRLDIAEAKVADLKAQGVIIQEAFANKDVKPMGSASEDQKGEVRDEIDFI
                     REQLMATQIYYEQGDTQSAYASARTAYLDSYEYVEIPLRVIAPDFTLQVEFEFATLRN
                     LINENAPFDEIDKAVIAIERSLDESERLVSGTGTIAPMIAFVSSFAIIFREGLESVLI
                     LGAIITYLEASRNIKFKKYVHYGIVLAIGATAVTWFIASYVIEISGANRELIEAIAAL
                     SATAVLFYVSFWILNKIEHKRWMEFVKAKVFQASASGGVTVFVLLSFFTVYREGFETV
                     LFYQAMFAFAKYMELYVALGFILGIVSLLAIYFAFRKLGKRLPLRALFGLTMGIGAYL
                     SIAFLGNAIREFQVLDYIPYTSMLGIIPRLDINVATMTGIYPTLETTIGQIVLLSVYV
                     VASLYVLILRPRRQKALATMRKSRAQVNE"
BASE COUNT          744 a          485 c          387 g          813 t
ORIGIN      
        1 atattccatt tgtaacttta agatagagta atgctccagc aacactcgcg gcagggcctg
       61 ataaaattgt gagtattggc tttgtccgaa aagtttccat acttgtcaca ccgccgtcac
      121 ctttcataat cattaatgga gctgtcacac cagcttttct aattgcactt tcaacaaatc
      181 cagcaacttc tacagttttt ggtagtacac ttgcattaat tgctgcagta agagttctaa
      241 tctctaaacc ataaattcca gaaatctcat gtgaagatgt agatggaatt ccagattttg
      301 ttgcattttc cataacaaac atctcatttg aaggatcgtc tacgccgaat gcctcagtag
      361 caacaataac ttttgcacca tcatttttta gttcttgaat agcctgagaa acctctgact
      421 cggttattag gtgagatgta tcaagaaatc tatgactagt tttgatgtcg ttttgagagt
      481 ttatttttgc gtcttgtaga tttgtccttt tgataacgtc ttttttttca ggacctactc
      541 ccattgctat aattcctact ttagcagtat cagactctaa aagtgaattt attgcttgag
      601 ttgtgctgtg tgcgatgatc tcaatctcgt ttaatttaat atcagaattc ttcaaaatgt
      661 tagaaagtgc taaaactatt ccgtcggaaa caccattttc agacgaatgt gttgtcggaa
      721 cagtagacaa cgaaataata tttcctgtaa ctatatcaat agcaacggct tttgtgaatg
      781 ttccaccgac atcaattcct attctaattc ttctttcatt catttacttg tgccctcgat
      841 tttctcattg tggcaagtgc tttttgtctc cttggtctta gaatgagaac atataatgat
      901 gccactacat agactgaaag caaaactatc tgtcctatag ttgtctccag tgtaggataa
      961 attcctgtca tggttgctac attgatatct aatcttggaa taattccaag catgctagta
     1021 tatgggatgt aatctaatac ttggaactct ctaatcgcat ttcctaagaa tgctatagac
     1081 aagtacgcac ctattcccat ggttaaacca aatagtgcac gtaatggcag acgttttcca
     1141 agttttctaa atgcaaagta aattgcaagt aatgatacta ttcctaaaat gaatcctaat
     1201 gcaacgtata gttccatata ttttgcaaat gcaaacattg cttgatagaa cagaactgtt
     1261 tcaaatcctt ccctataaac agtgaaaaat gaaagcaaga caaacaccgt cactcctcca
     1321 gaagcagaag cttggaaaac tttggctttt acaaattcca tccatcgctt gtgctcaatt
     1381 ttattaagta tccaaaaact gacatagaac aaaactgcag tcgcagataa ggcagctatt
     1441 gcttcaatta attctctatt tgcacctgaa atttctatca cgtaagatgc gataaaccag
     1501 gtaactgcag ttgcaccaat agcaagtacg ataccataat gtacgtattt tttgaatttt
     1561 atgtttcttg atgcttcaag atatgttatg attgcaccta agattaagac tgactctaaa
     1621 ccttctctaa atattatagc aaaagatgaa acgaatgcaa tcattggtgc tatagttcca
     1681 gttccagaaa caaggcgttc tgattcatca agactccgct ctattgcgat tacggcttta
     1741 tcaatttcat caaatggagc attttcatta attaaatttc tgagtgttgc aaattcaaat
     1801 tcaacttgca acgtaaaatc aggtgcgatt acacgtaaag gaatttcaac gtattcgtaa
     1861 ctgtcaagat atgctgttct tgcactagca tatgcagatt gagtatcgcc ttgttcatag
     1921 taaatttgag tggccattaa ttgttcgcgg ataaaatcaa tctcatctcg gacttcgccc
     1981 ttttgatctt cactagcaga acccattggc ttaacgtcct tatttgcaaa cgcttcttgt
     2041 ataatgacac cctgggcctt caagtctgca acttttgctt ctgcaatatc taatctagga
     2101 ataatagagt caaccaaaaa tgcgcgtatt gtttctggtg attcttgatt tttaatcatt
     2161 gcacgcaatt cttcacgcat atcccattct aactggtaaa gtaattcagc gtctgcaatt
     2221 tcaatttcag gttctaaata ttcaaagttt tcaagatatg cctctattcc aatttcttca
     2281 gccttatcat aatttccatt atcaagttca atcaataatt ctccatacaa atctcgaata
     2341 atattataca gattagaatt atctattcca gcagtagtta aaacatcagt tccaagtaaa
     2401 tctctctgga tgaaagagat taacgtacc
//
LOCUS       DASCPJ010000053         3197 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000882811, whole genome shotgun sequence.
ACCESSION   DASCPJ010000053 DASCPJ010000000
VERSION     DASCPJ010000053.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000882811"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(140..262)
                     /locus_tag="QGF01_02440"
     CDS             complement(140..262)
                     /locus_tag="QGF01_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19790.1"
                     /translation="MKISDIETIYIKEKKIEKRTDGSQDALLVKITTDSGIIGW"
     gene            complement(278..1021)
                     /locus_tag="QGF01_02445"
     CDS             complement(278..1021)
                     /locus_tag="QGF01_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HJN19791.1"
                     /translation="MVVSSFKPTSEVLAIPVNILPKNWQIDAYTNLSNYTEYDFTRYF
                     INSFIVTFFAVLLAVILCTTAGYGFAKYKFKYNNLIFIIVLSTIMIPFQAIVVPLFIL
                     MKDLGLQNSYLGLIIPESLTAFGVFLMRQFFYSIPDEIIESARIDGASEFRIFWTISV
                     PMAKTAIFALIIFHSQWVWNLLLWPLIIISSPEMRTIPQAIALFSGVYFTPYPEQLAI
                     SVLACLPLLVIFMFLSKNFTKGITLTGLK"
     gene            complement(1091..1960)
                     /locus_tag="QGF01_02450"
     CDS             complement(1091..1960)
                     /locus_tag="QGF01_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HJN19792.1"
                     /translation="MKYNEKIQKKGFYFVLPTLIIYGIFLIYPMLSAFHYSFYKWNMI
                     SESKYVGLKHYIRMFGDRRFWNSYLTTFHFTFISIFIIIILSFLLALLFQEKIMLKNF
                     FQSSIFVPVILTMVAIAVVWQFMFQSTGVLSNIFLDIFGINIKWLTSKSVAPYSMILV
                     NVWKITGYYMIIFIAGLLNIPSIYYEAAKVDGTSYLQRLFYITIPLMKNTFILAFVSG
                     VIFSFAAFPQQYVMTEGGPARSTEVLALLIYKQAFEFTKFGYSSAISVTFFLTLLVFS
                     LIQFRIFQSESNY"
     gene            complement(2046..>3197)
                     /locus_tag="QGF01_02455"
     CDS             complement(2046..>3197)
                     /locus_tag="QGF01_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013697.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HJN19793.1"
                     /translation="DKIIMQTAKDFMAKNPNVEIKVQLVADADMPTKVRTALTGDGVD
                     TFAMPNMQSPWFMANGTTAEIIPSAFGKSTVQEVVDMWLPGAIKKTGGFYEGKYYGIP
                     HEMSNYAAWVNTAHMKAAGLDPKKDIPKTWQEFKLIGKMLTKKNNDVITRNGFAINTK
                     AAIFPFLILHTLMQQKGQDWSSEKGLHKSLSTKGGLEAFSTFTNWATKDGIFDPGLFD
                     NEREGFGNGLTSQFLTGGTWYWGVLDGYSVKRNEVMPIKYPRFKDGENIGGVAYGYCV
                     FVAKQTKNKEMAWKWLNYLESHPEKYIVHGWFQPRKSLDKTLAAKYIPNYEVFGEEFE
                     YAASIIATANFAELQDAVGAAVQRVIFEGTSNEDSLSMLKDDVENILDM"
BASE COUNT         1098 a          557 c          460 g         1082 t
ORIGIN      
        1 tttgatacat tttgtcccac aaactttcga tttctaaagg attttcacca acaataatat
       61 tttttaaacc atcaacaagc ttatgtgaat agggagcatt aattattgca tcagcgacta
      121 caggacaact atcaacttct caccatccaa taataccaga gtccgtagtg atttttacta
      181 aaagtgcatc ttgagaaccg tcggttcttt tttcaatctt tttttctttt atataaatag
      241 tttcaatatc tgaaattttc ataagtattt tttcggatta ttttaatcca gttaaagtta
      301 tacctttagt gaaatttttc gataaaaaca taaatatcac tagtagaggt aagcaagcca
      361 gaacacttat agctaattgt tctggatatg gagtaaagta cacaccagaa aaaagtgcta
      421 ttgcttgagg gattgttctc atttctggag aagagataat tattaaaggc cataaaagta
      481 aattccacac ccactgagaa tgaaatataa tgagtgcaaa aatagctgtt ttagccatcg
      541 gtactgatat agtccaaaaa atcctgaatt cagacgcacc atctattctt gctgattcaa
      601 taatttcatc aggtatagaa taaaaaaact gtctcattag aaatactcca aatgccgtta
      661 gactttctgg aataataagt cccaagtaag aattttgtag gcctaagtct ttcataagaa
      721 tgaaaagtgg tactacgata gcttgaaaag gtatcattat tgttgaaagg acaataatga
      781 agattaagtt attgtattta aatttatatt tggcaaaccc atatcccgca gtagtgcaga
      841 gaataacggc taacaagaca gcaaaaaatg ttacaatgaa gctgtttata aaatatcttg
      901 taaaatcata ctctgtataa tttgacaaat ttgtataagc atcaatctgc caattcttag
      961 gtaaaatatt tacagggatt gctaaaactt ctgaagttgg tttgaatgat gatacaacca
     1021 tccagaaaaa tgggaataaa aatattatta ttagtaatac taatgaaaag tactttaata
     1081 tccttaacat ctaataatta gattctgatt gaaaaattct aaactgtatt agagaaaaaa
     1141 ctagtaatgt taaaaagaaa gttaccgata tggcagacga gtaaccaaat tttgtaaact
     1201 caaatgcttg tttatagatc aacaatgcca aaacttctgt agatcgagcc ggtccacctt
     1261 cagtcataac atattgttgt ggaaatgccg caaatgagaa aattacccct gatacaaatg
     1321 caagtataaa ggtgtttttc attaagggga tagtaatata gaacaatctc tgtaaatatg
     1381 atgtcccatc aactttagca gcttcataat aaatactcgg aatatttaaa agacccgcaa
     1441 taaatataat catataatac ccagttatct tccaaacatt tacaagaatc attgagtatg
     1501 gtgctacgga tttagaggtg agccatttta tatttattcc aaaaatatct aaaaatatat
     1561 tagataatac cccagttgat tggaacataa attgccaaac aacagctatt gcaaccatag
     1621 ttaaaataac agggacaaat atagatgatt gaaaaaaatt tttaagcatt attttttctt
     1681 gaaataataa tgctaataaa aatgataaaa taataattat aaaaatactt ataaatgtaa
     1741 aatgaaatgt tgttaaataa gaattccaaa atcttctgtc accaaacatc ctaatatagt
     1801 gtttcaatcc aacatattta ctctcactaa tcatattcca tttataaaat gaatagtgga
     1861 atgcggatag cattggatat attaaaaata ttccatatat gattaatgtt ggtaaaacaa
     1921 aataaaaccc ttttttttgg attttctcgt tgtacttcat tacttgtttt atctataatt
     1981 tttattacta ctatttacca attctattta ttggcactgc ttttagcagt gccaataaaa
     2041 ttaaattaca tatctaaaat attttccaca tcatctttaa gcattgataa agagtcctca
     2101 tttgatgtgc cttcgaatat aactctttga actgctgcac caacagcatc ttgtaactca
     2161 gcaaagtttg cagttgctat tatggaggca gcatactcga attcttcacc aaacacctca
     2221 tagtttggaa tgtactttgc ggcaagagtc ttatctaagc ttttccgtgg ctgaaaccag
     2281 ccatgtacaa tatatttttc aggatgactt tctaaatagt ttaaccattt ccaagccatt
     2341 tccttgtttt ttgtctgttt tgcaacaaat acacagtaac catatgctac accaccaata
     2401 ttttcaccat ctttaaatct tggatatttt attggcataa cttcatttct tttaacagag
     2461 tatccatcta aaactcccca ataccaagtt cctccagtta aaaattgtga tgttaaacca
     2521 tttccaaatc cctctctttc attgtcaaaa aggcctggat caaagatgcc atcttttgtt
     2581 gcccaattag taaaagtaga gaaagcttcc agcccacctt tagtacttaa acttttatgc
     2641 agtccttttt ctgacgacca atcctgcccc ttttgctgca ttaaagtatg taatattaga
     2701 aatggaaata tagccgcttt tgtgtttatt gcaaatccat ttctagtgat aacatcatta
     2761 tttttttttg taagcatttt cccgattaac ttgaattctt gccaagtttt gggaatatcc
     2821 tttttggggt ctaatccggc ggctttcata tgtgccgtat ttacccatgc ggcataattg
     2881 ctcatttcat gtgggattcc ataatatttc ccctcataaa aaccaccagt tttctttatt
     2941 gcacctggta accacatatc tacaacttct tgaacagtgc ttttgccaaa tgcagatggg
     3001 attatttctg ccgttgttcc attcgccata aaccaaggtg attgcatatt aggcatagca
     3061 aaagtatcga caccatctcc tgtgagtgca gtccggactt ttgtaggcat atccgcatct
     3121 gcaactagtt gaactttaat ttctacatta ggattttttg ccataaaatc tttagctgtt
     3181 tgcataataa ttttatc
//
LOCUS       DASCPJ010000054         8009 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000882921, whole genome shotgun sequence.
ACCESSION   DASCPJ010000054 DASCPJ010000000
VERSION     DASCPJ010000054.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000882921"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="QGF01_02460"
     CDS             complement(<1..480)
                     /locus_tag="QGF01_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HJN19794.1"
                     /translation="MTEQLSDSDGTFLVKIARKAATEFLSNGNKIKLDTDNEKKFSFN
                     SGVFVTLNKHQELRGCIGFPMPDKKLSHAIVDGAIAASTQDPRFQPVKIKELDDIVFE
                     VTVLTPPSEIDVSNPTEYLEKIKIGRDGLIVSHSFYSGLLLPQVPGEYGWSVEEFLQH
                     "
     gene            541..750
                     /locus_tag="QGF01_02465"
     CDS             541..750
                     /locus_tag="QGF01_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19795.1"
                     /translation="MAEPILALTALALITIALIGQGIEMRKIRIRTYGEDGVGHPNIF
                     LDKKNVKWYILIAAGLGLAFSAQYV"
     gene            complement(747..1064)
                     /locus_tag="QGF01_02470"
     CDS             complement(747..1064)
                     /locus_tag="QGF01_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HJN19796.1"
                     /translation="MSDIIPPFKAILETKGRKTGKTYKTELLVVRYNNKFYFSRRNPN
                     SDWLKNAIKNSDVKVSFDGQTFAGKANLVKDDLLNKKISQLKYKDEKRANEARVVLEV
                     DCS"
     gene            1154..1606
                     /locus_tag="QGF01_02475"
     CDS             1154..1606
                     /locus_tag="QGF01_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HJN19797.1"
                     /translation="MSTHLEGMPEHLQTADEFRGKPVVDREGIKYGKIKHIHIHSDTL
                     SVAGVTVHQGFHKDYYLSNDSIDKFTEKTLLLSTPPIRVGVQVVDIDGEKIGKVKKLH
                     RHPDTNELEFIEIPAGLIHKKMIPKSDIWGVGEKIILNFTKKEFNKLE"
     gene            1971..2978
                     /locus_tag="QGF01_02480"
     CDS             1971..2978
                     /locus_tag="QGF01_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJN19798.1"
                     /translation="MHAIEVDSLTKKFGDFKAIDNISFDVEEGEIFGFLGPNGAGKST
                     TMMILTTLLKPSSGKVLVGGHDITSDAKKVREKIGYVQQEISVDEFLTGRENLYLHAR
                     INQIPGNLIKSRIDDVLELVELGEKQDHATLTYSGGMRKRLDIANGLLSRPSVLFLDE
                     PTVGLDIQTRRKIWSYIKKIRKDFGMTVFISTHYMEEADDLCDRIGIIDFGKIQVIDT
                     PKSMKRAIGNEIISFNLVDGKANQDTLIDQINNIEFVKEVKNKQDLITVFSTNSNEVI
                     PKIFQASTNLNMKINSLTLKQPTLDDVFISYTGHDLRDETENKKYSRRKEFNLNRRKG
                     L"
     gene            2978..3748
                     /locus_tag="QGF01_02485"
     CDS             2978..3748
                     /locus_tag="QGF01_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJN19799.1"
                     /translation="MTDTYSIFWREMKRYKKSRGGFIIRLIQPAVWIIVMGNIFSGTQ
                     PLIQSVGFDGEYIEFMTPGIIILTAIFTSIFGGVNTLWDRRYGFMNQALTSPISRSSI
                     ALGKMLAISAVAAIQASLILGIALAIGVTMPNLFMIAPIMAIVVLFSIGFSGISVMFA
                     AVAKSQETFWGTVNFLGLPMFMISPALFPLALMPDWLATIAHFNPVTYAIILVRSMMS
                     GFVEGSSAALSIVVLSAFVIAMTLLASYVFTREVNKPF"
     gene            3822..7322
                     /locus_tag="QGF01_02490"
     CDS             3822..7322
                     /locus_tag="QGF01_02490"
                     /inference="COORDINATES: protein motif:HMM:NF028479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HJN19800.1"
                     /translation="MKKLALIGLLFGILLVGAIPNGLSASDHRYASEWGGYSMSNDGE
                     FFRPQYVAIDDDGNVYVTDLSSRVQKFTSDGTFLKSWGSMGPVDGQFGSPSGIAVFGD
                     HVYVADKKQHSIDVFDKDGNFVFSWGEFGSGDGQFSKPHGVKINSDGIVFVADPGNHR
                     IQKFTTDGEFLSAFGIFGTTDGKLKTPIQLAFHEDSVYVADPGNLKIEKYTDDGVFLK
                     SFDQRFGGSNISPSDIVIDSDGDIYVTDLFKHRIVKISSEARTLKTFGHAGNEKNQFL
                     EPTGIAIDAKGYLLVVDSVKNRIVKFETPISKQIEEALTAQQVEKLKELSYTEESENT
                     DIEPVDQTTETTEESTEPEQQFVHDTTKPLLQVPNDLVIESTGSFTQVDIGEAMAMDD
                     NGIRLLINNSPESFSLGTYQIIWTAVDNSGNSAFAIQEIHVVDTIPPVISQIQDKTLE
                     AAAPIENIVNLQTPTATDVSGVVSIISDTPEFFPIGETIVTWTATDVAGNESSIEQRV
                     TIVDTTSPSLQIPADIEIEATSIDQNIIDLGEVTATDNGEIISITNDAPEFFVVGNTE
                     VIWSVIDSSGNLSSGSQMISIIDTTVPIITPAIDITLEAISIDENISDLIVPSVYDIH
                     DISITNDTPEFFPIGETIVTWTATDVAGNESSIEQRVTIVDTTSPSLQIPDQKTVEAT
                     SLQNTLVEIGEAQADDISGISSITNNAPESFSLGTTLVTWTAVDNYGNTATHDQTITV
                     VDTTAPSIFVPDDIFSEAIDPTLNYIDIGQASGFDPTSAVSISNDMPTSFPFGSTTIT
                     WTAVDDSGNVSTDTQTVTIVDTTVPEILSPSDIVVEATGNFVTDIELGLAIIDDIIEV
                     NTVTNDAPDSFPLGETTVTWTATDSSGNFAISTQTVSVVDTTAPSITAPNAIQVEATG
                     IEGNLADIGISTASDSVEIISITNDAPDSFPLGETTVTWTATDSSGNFAISTQTVSVV
                     DTTAPTLTIPENIIVDSFTLEKLVVIGEAYAFDSVDTLPILTNDAPEEFPLGNTVVTW
                     SVIDQFGNSTTLEQTVSVQACGQSISYYNQIMGTTEDDVLMGTGTADLIFGFSGNDMI
                     IGGAGNDCIIGGEGDDVIFGNEGNDHLVGGEGTDIIKGNSGDDKLTGGFGYDILDGGD
                     DFDISYDSVSDIVIKCEEQL"
     gene            7376..>8009
                     /locus_tag="QGF01_02495"
     CDS             7376..>8009
                     /locus_tag="QGF01_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJN19801.1"
                     /translation="MVVKTAEIDNNKIRYLEEGSSENTLLLLHGLGASAERWEHVIPL
                     FAKEYRVIVPDLVGFGHSDKPMVDYTMEYFSEFTSKFVESIGIKKLNIIGSSLGGQIA
                     AEYAVNHNDKIRKLVLVSPSGVMKYATPALNSYTSAALYPNNDAALTAFQTMSCQDDI
                     DEKMVSEFVERMQLPNAKMAFMSTLLGLSNAKVITEKLNMITVPTMIVWGE"
BASE COUNT         2681 a         1359 c         1496 g         2473 t
ORIGIN      
        1 atgttgtaaa aattcttcca cactccaacc atattcacca ggtacctgtg gcaaaagcag
       61 tcccgagtaa aaagaatgac ttacaattag accatcccta ccaatcttga ttttttccaa
      121 atattcagtg ggattagaaa catcaatctc acttggaggt gttaagactg tcacttcaaa
      181 tacaatatca tccagttctt tgatttttac tggttggaat ctgggatcct gagtagaagc
      241 agctattgct ccatctacta ttgcatgtga tagttttttg tctggcatag gaaatccaat
      301 acatcctcgc aattcttgat gtttgttgag tgtaacaaac acaccagagt taaatgaaaa
      361 ttttttctca ttatcagtat ctaattttat tttgtttcca tttgaaagga actcagtagc
      421 agcctttctt gctattttga ctagaaaagt tccatctgaa tcagataact gctctgtcat
      481 gaagtactca actgacatgt attaaaaaaa cattaaattt tacaaaagat tggataatat
      541 atggcagagc caattttagc acttactgca ttggcattaa tcacaattgc attgataggg
      601 cagggaatag aaatgcgaaa aatacgcata agaacttacg gtgaggatgg agttggacat
      661 ccaaacatat tcttggacaa aaaaaatgta aaatggtata ttcttatcgc cgcaggtctt
      721 gggttggcat tttctgctca gtatgtttaa ctacaatcta cctctaagac aactcttgct
      781 tcattagcac gtttttcatc cttatacttt agttgagata tttttttgtt aagcaagtca
      841 tcctttacaa gattagcctt tcctgcaaat gtctgcccat caaatgatac ctttacatcc
      901 gaatttttta ttgcattttt tagccagtcg ctgtttgggt ttctacgtga gaaatagaat
      961 ttattgttgt atctgaccac aagtaattca gttttgtatg tttttccagt tttcctacct
     1021 tttgtttcca aaattgcctt gaatggagga atgatgtcac tcatatcaag tttggatcaa
     1081 gtcaagtaat ttaactacat tgattgaaaa ttctgatatt caattagata aaataatcta
     1141 gcaagtcaat tgcatgtcta cacacttaga aggaatgccg gagcatctgc aaaccgcgga
     1201 tgagttcaga ggtaaacctg tagttgatag agagggaatc aaatatggaa aaattaaaca
     1261 catccacata cactctgata cactttcagt ggctggcgtt acagtgcatc aaggatttca
     1321 taaagattat tatctttcaa atgattccat tgataaattc acagaaaaaa cattgctttt
     1381 gagtactcct ccaattaggg ttggtgttca agtcgttgat atagatggcg aaaaaattgg
     1441 taaagtaaaa aaattacatc gtcatcctga taccaatgaa ttagaattta ttgaaatacc
     1501 agctggacta atacacaaaa aaatgatccc aaaatctgat atttggggag ttggagaaaa
     1561 aataattcta aatttcacca aaaaagaatt caacaaacta gaataaccac ttttttcatt
     1621 tctaattttt atttttaaaa atggatcact tatctatcat tcgcgtttct tttatgcaaa
     1681 ttttgctaat tacacatatt cgacaagtca acgtcagacc atagttgtcc cttttcgaat
     1741 agagtcatca cgggctctcg tttttagaga gccctttttt tatgtttgat tgcttatgaa
     1801 tacagctaga acaaagaatg tcatagcaat tgaagcacct acacccatga taaaaataat
     1861 cttattgcca gtcattcaca ctaatcttga aatgatagaa tctaaatagc tttaatttcc
     1921 aatatttcaa attagtaatc aaattataat tgtgaaaatc agacctaaca ttgcatgcaa
     1981 tagaagttga ttcattaaca aagaaatttg gtgattttaa ggcaatagac aatatttcct
     2041 ttgatgtaga ggagggagag atttttggat ttttaggtcc aaacggtgca ggtaaaagca
     2101 ccacaatgat gatacttaca acactgctaa aaccatcatc aggaaaggta ctggtaggcg
     2161 gtcacgatat tacgtctgat gccaagaagg taagggagaa aattggttat gttcaacagg
     2221 aaatcagtgt agatgaattc ctaaccggta gagaaaatct atacttacat gcacgaatta
     2281 atcaaattcc aggcaatctg atcaaatcac gaatagatga tgtgttagaa ctggtggagc
     2341 tgggtgaaaa acaggaccat gcaacattaa cctattctgg cggaatgcga aagagacttg
     2401 acatagccaa cggattgtta agcagacctt ccgtactatt tcttgacgag cctactgttg
     2461 ggcttgatat acaaacaagg cggaagatat ggagctatat aaaaaaaatt cggaaggatt
     2521 ttggcatgac agtttttatc agcactcatt acatggagga agcagatgat ctatgtgaca
     2581 gaattggaat aattgatttt ggaaaaatac aagtcattga cacaccaaaa tcaatgaaga
     2641 gggctatagg aaatgaaata atctctttta atcttgttga tggtaaagca aatcaagata
     2701 ctttaattga tcaaattaat aatattgagt ttgtaaagga agtaaaaaat aaacaagatt
     2761 taatcactgt attttctaca aacagtaacg aagtaatccc aaaaatattt caggcttcta
     2821 ctaatcttaa catgaaaata aattctctta cgttaaaaca gccaacactt gatgatgtct
     2881 ttatctcata cacagggcat gatttgagag atgaaactga aaataaaaaa tacagtagaa
     2941 gaaaagaatt caatctgaac aggagaaaag gactttgatc actgacactt actcaatttt
     3001 ttggcgtgag atgaaacgct ataaaaaatc aagaggtggt tttataatta gactgattca
     3061 gccagctgtt tggatcatag ttatgggtaa catattttca ggaacacaac ctctaattca
     3121 atcagttgga tttgatggtg agtatataga attcatgaca ccgggaatca taatactcac
     3181 tgcaattttc acaagtatat tcggtggtgt aaacactttg tgggatagaa gatacggttt
     3241 catgaatcag gcgcttactt ctccaatctc taggtcatcc atagcacttg ggaagatgct
     3301 tgcaatatct gcagttgcag ctattcaggc cagtcttatt ttgggtattg cacttgcaat
     3361 aggtgttaca atgccaaatt tgttcatgat agctccaatc atggcgatag tagttttatt
     3421 ttctattgga ttttctggaa tatctgtcat gtttgcggca gttgcaaaat ctcaagaaac
     3481 tttttgggga actgtaaatt ttcttggttt gcctatgttt atgataagtc cagctctttt
     3541 tccattagcc ctgatgccag attggctggc taccattgct cattttaacc cggtcacgta
     3601 tgcgataatc ttggttcgaa gtatgatgtc tggttttgtc gaaggctctt cggctgcact
     3661 tagtatagtc gttttatcgg catttgttat agccatgaca ttattggcta gttatgtatt
     3721 cacgcgtgaa gtgaacaaac ccttctgatt taaccaaatt tgacctaaaa acgaagataa
     3781 tcaaacatag aagatcttgt gttactctga gaccataatt attgaaaaaa ttagcattaa
     3841 ttggattact ttttggtata cttctagtag gggcaatacc taatggatta tctgcatctg
     3901 atcatcggta tgccagtgaa tggggaggat acagcatgtc aaatgacggt gaattttttc
     3961 gacctcagta tgtagctatt gatgatgatg gtaatgtcta tgtcacggat ctaagttcta
     4021 gagttcaaaa atttacatct gacggaacat ttctgaaatc ttggggttct atgggtccag
     4081 tagatggtca atttggttct ccaagtggta tagcagtatt tggtgatcat gtctatgttg
     4141 cagataaaaa gcagcattcc atcgatgtat ttgacaagga tggtaacttt gtttttagtt
     4201 ggggagaatt tggaagtgga gacggacaat tttctaaacc acatggagta aaaattaatt
     4261 cagacggaat agtttttgtt gcagatccag gtaatcatag aattcaaaaa tttactactg
     4321 atggagaatt tttatcagca tttggtatct ttggcaccac tgatggaaaa ttaaaaacac
     4381 ctattcaact tgcatttcat gaagattcag tttatgttgc agatccaggc aatctaaaaa
     4441 ttgaaaaata tactgatgat ggagtttttc taaaatcatt tgatcaacgc tttggtggtt
     4501 caaacataag tccatctgac atagttattg attcagatgg cgatatttac gtaacagatt
     4561 tgttcaagca tagaatagtt aaaattagct ctgaagctag gacactaaaa acattcgggc
     4621 atgctggcaa tgaaaaaaat caattcctcg aaccaactgg tattgctatc gatgcaaaag
     4681 gatatctctt ggttgtagac tctgtcaaaa acagaatagt aaaatttgaa acaccaattt
     4741 caaaacaaat tgaagaagca ttaactgcac aacaagttga aaaattaaaa gaattatcat
     4801 acacagaaga atctgaaaac acagacatag aaccagttga tcaaacaaca gaaactactg
     4861 aagaatcaac tgaacctgaa caacaatttg tccatgatac gacaaaaccc cttttgcaag
     4921 tgccaaatga tttggttata gaatcaactg gtagtttcac acaagttgat attggtgagg
     4981 caatggctat ggatgataat ggtattagat tgttaatcaa taactcaccg gaatcattct
     5041 cattaggcac atatcagata atatggaccg cagttgataa tagtggcaat tctgcctttg
     5101 ccatacagga aatacacgtg gttgatacta taccacccgt aatctcacaa attcaagata
     5161 aaactctcga agcggccgca ccaatagaaa atatcgttaa cttacaaaca ccaaccgcaa
     5221 ccgatgtatc tggcgtggtt tctattatta gcgacactcc tgaattcttt ccaattggtg
     5281 aaactatagt tacatggact gcaactgatg tagcaggaaa tgagtcaagt atagagcaaa
     5341 gagtaactat agttgatacc acatcaccat ctttacagat acctgctgat atagaaattg
     5401 aggcaacaag tattgatcaa aatattatag atcttggtga agttacagca actgacaacg
     5461 gcgaaatcat ttcaataaca aatgatgcac ctgaattttt tgtagttggt aatacagaag
     5521 ttatatggtc agtaattgat tcatcaggaa atctatcaag tggtagtcaa atgatttcta
     5581 taattgatac aacagtacca atcataacac cagcaattga tattactctt gaagcgattt
     5641 caattgatga aaatatatct gatcttattg ttccatctgt ttatgatata catgatattt
     5701 ccattacaaa tgatactcct gaattctttc caattggtga aactatagtt acatggactg
     5761 caactgatgt agcaggaaat gagtcaagta tagagcaaag agtaactata gttgatacca
     5821 catcaccatc tttacagata cctgaccaaa aaactgtaga agctactagt cttcaaaaca
     5881 ctctagtaga aattggtgag gcacaagctg atgatatatc tggaatttct tcgatcacaa
     5941 ataatgcacc tgaatcattt tcattgggta caacactagt tacatggact gcagttgata
     6001 actatggtaa tacagcgaca catgatcaaa caataactgt tgttgatacg accgcaccat
     6061 caatttttgt cccagatgat attttttctg aagccattga tcctactctg aattacatag
     6121 atattggaca agccagtggt tttgatccta ctagtgctgt atctatcagt aatgatatgc
     6181 ccacttcatt cccatttgga agtacaacca tcacctggac tgcagtagat gattcaggca
     6241 atgtttcaac agatactcaa actgtgacca tagtggacac aactgttcct gagatactct
     6301 caccttctga tattgtagta gaggcaactg gtaattttgt tactgatata gaattaggtc
     6361 tagcgataat agatgatata atagaagtca atactgtcac taacgatgcc ccagattcat
     6421 ttccactcgg tgaaaccaca gtaacatgga ctgcaactga ctcatcaggt aattttgcaa
     6481 tatcaacaca gacagtttct gtagttgaca caactgctcc aagcattacc gctccaaatg
     6541 caattcaagt cgaagcaaca ggcattgagg gaaacttggc tgacattgga atatcaaccg
     6601 ccagtgattc tgtagagatt atttcaataa caaatgacgc accagattca tttccactcg
     6661 gtgaaaccac agtaacatgg actgcaactg actcatcagg taattttgca atatcaacac
     6721 agacagtttc tgtagttgac acaactgctc caacattaac aattcctgaa aatattatag
     6781 ttgatagctt tacactcgaa aaattagtag taataggaga ggcatatgca tttgattctg
     6841 tagatacttt acccattcta acaaatgatg ctccggaaga atttccttta ggaaatacag
     6901 ttgttacatg gagtgttatt gatcaatttg gtaattccac aactttagag cagacggtat
     6961 ctgttcaagc atgtggacaa tcaatatcat attataatca aatcatggga actaccgaag
     7021 atgatgttct tatgggcacc ggtacagcag atcttatctt tggatttagc ggaaatgata
     7081 tgatcatagg tggagcaggt aatgattgta taattggagg tgaaggtgat gatgttatat
     7141 ttggaaacga aggcaatgat catcttgtag gtggagaagg cactgatatc attaagggta
     7201 attctggaga tgacaaactc acaggtggat ttggttatga tattcttgat ggaggagatg
     7261 attttgacat atcatatgac tctgtttcag acattgtaat caaatgtgaa gaacagttat
     7321 agatctatta ttatagataa aatcttatat tttctgtatt tttaatcagc ttggtatggt
     7381 tgtaaaaaca gctgaaattg ataataataa aatacgttat ttggaggaag gaagttcgga
     7441 aaacactctc cttctacttc atggattggg ggcttctgca gaaaggtggg agcatgtaat
     7501 accacttttt gctaaagaat acagagtaat tgttcctgat cttgtaggtt ttgggcacag
     7561 tgacaaacca atggtagatt ataccatgga gtatttttca gaatttacct ccaaatttgt
     7621 agaaagtatt ggaatcaaga aactgaatat tattggttct tcacttgggg gccagattgc
     7681 agcagaatat gcagtgaatc ataatgacaa aataagaaaa cttgttcttg tttcaccatc
     7741 tggagtgatg aagtatgcaa cacctgcact gaatagctat acctcagctg cattatatcc
     7801 aaataatgat gccgcattaa ctgcattcca aaccatgtca tgccaggatg atattgatga
     7861 aaaaatggtc tctgaattcg tagaaaggat gcagttacca aatgcaaaga tggctttcat
     7921 gtcaacactc ctaggtttga gtaatgccaa agtgatcact gaaaaattga acatgatcac
     7981 agttcctact atgattgtat ggggtgaaa
//
LOCUS       DASCPJ010000055         1758 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000887191, whole genome shotgun sequence.
ACCESSION   DASCPJ010000055 DASCPJ010000000
VERSION     DASCPJ010000055.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000887191"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="QGF01_02500"
     CDS             <1..230
                     /locus_tag="QGF01_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19802.1"
                     /translation="PGVSSSLQDLTGVLNDVMTTSTFDPESIISNDHLDQDTTAILEE
                     AHAVIEGETISKMPEPPAIFTQIAKEKKFMI"
     gene            complement(220..561)
                     /locus_tag="QGF01_02505"
     CDS             complement(220..561)
                     /locus_tag="QGF01_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19803.1"
                     /translation="MEEFDIKLKLSEAPTVTQTKKLKNYFKDMPVDEIISGLKFANSR
                     WIAKDAGVLNVGRKSILKKEIHSVTPEQAQWRLKNWKMMIANYRRRGYSYPTISRIKN
                     NLRQISKKTKS"
     gene            840..1580
                     /locus_tag="QGF01_02510"
     CDS             840..1580
                     /locus_tag="QGF01_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HJN19804.1"
                     /translation="MMPAQQGYDRAITVFSPDGRLYQVEYAIETVKRGTLAIGIKTKD
                     GIIIAAEERPRKLQIDESPQKLFQVDHHIGIAAAGYIPDARNQVDDARFFSQSNKLVY
                     DEPVDVETVAKHLADQAQHYTQYAGVRPFGVALIIGGIDQQGSSLFLTDPSGTYIPYN
                     AVAIGSNSDKVTEFLRKEYKHDITVDDAKILAIAGINQVAENTEGSNHVKMSQIINDT
                     KHFKILSGEEIAKIAQISKEKYAIEPKK"
     gene            1610..>1758
                     /locus_tag="QGF01_02515"
     CDS             1610..>1758
                     /locus_tag="QGF01_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA uridine N3 methyltransferase"
                     /protein_id="HJN19805.1"
                     /translation="MNLSIAIPDSLLVDEATKIDKTKKISIIARTCAIFRVREIFIYR
                     EGGGH"
BASE COUNT          656 a          258 c          287 g          557 t
ORIGIN      
        1 tgccaggagt tagttcttca ttacaagatt taactggagt gttaaatgat gtaatgacaa
       61 catcaacatt tgatccagaa tcaattatat caaatgatca tctggatcaa gataccacag
      121 caatattaga agaggcacat gctgttattg aaggtgaaac aatatctaaa atgcctgaac
      181 cgccagcaat atttacacaa attgctaaag aaaaaaaatt catgatttag tttttttact
      241 gatctgacga aggttatttt taattctaga aatggttggg taactataac cacgtctacg
      301 ataatttgcg atcatcattt tccagttctt caatctccat tgagcctgtt caggagtgac
      361 tgaatgaatt tcttttttca atatgctttt tctgcctaca ttcaacacac ccgcatcttt
      421 ggctatccaa cgactatttg caaattttaa tccagaaatt atttcatcta caggcatgtc
      481 cttaaaataa ttttttaatt ttttagtttg agtgacagtg ggtgcttcag ataatttcaa
      541 tttaatatca aattcttcca tattcatgaa tgattattat tatttaataa aaatgagatt
      601 aattagaatt cacaatcaaa agaaaaatac aagcgtaaat taacatataa tcgtaaaaaa
      661 accattacaa attatgttat gcacggttga aataaaaaat ccagaaatga acactcctat
      721 tgcaataaca atgatttttt gtaggtgttt catacagtat aagatcctaa attacatatg
      781 aattatggta ctaaaccatc aactagactt aaattaaact ttcattgaga tgaattaatt
      841 tgatgccagc acagcaaggt tatgatagag caattacagt tttttcacct gacggtcgat
      901 tatatcaagt tgaatatgct attgaaactg taaaaagagg tacattagca attggaatta
      961 aaacaaagga tggtattatc atagcagctg aagaacgacc tagaaaatta caaattgacg
     1021 aatcaccaca aaaattattt caagtagatc atcatatagg aatagctgca gcggggtata
     1081 ttccagatgc tagaaaccaa gtggatgatg ctaggttttt ctcacaaagt aacaaattag
     1141 tgtatgatga acctgtagat gttgaaactg ttgcaaaaca tttagctgat caagctcaac
     1201 attatacaca atatgcaggt gtacggccat ttggtgtggc attaattatt ggtggaattg
     1261 atcaacaagg atcctcatta tttttaactg atccaagtgg aacatatatt ccctataatg
     1321 cagttgcgat tggatctaat tctgacaaag ttacagaatt tttacgaaaa gagtataaac
     1381 atgatatcac tgttgatgat gcaaaaattt tagcgatagc aggtataaat caagttgctg
     1441 aaaacactga aggttctaac catgttaaaa tgtctcaaat aataaatgat acaaaacatt
     1501 ttaagatatt atctggcgag gaaattgcta aaattgcaca aatttcaaag gaaaaatatg
     1561 caatagagcc aaaaaaataa ctgtgatttt ataataatat aactggataa tgaatttatc
     1621 aattgctata cctgattcat tattagttga tgaagcaact aaaattgata aaacaaaaaa
     1681 aatttctata atagcacgta catgtgcgat attcagggtt agggaaattt ttatttatag
     1741 agaagggggt gggcacag
//
LOCUS       DASCPJ010000056         7658 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000889229, whole genome shotgun sequence.
ACCESSION   DASCPJ010000056 DASCPJ010000000
VERSION     DASCPJ010000056.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000889229"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /gene="glyS"
                     /locus_tag="QGF01_02520"
     CDS             complement(<1..647)
                     /gene="glyS"
                     /locus_tag="QGF01_02520"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HJN19806.1"
                     /translation="MTYDDVMKLALERGFYFPSSEIYADAQAGFWEYGPSGVSLKNKF
                     LELWRRELVRRDKMIEIDGSQIMSKSVFDASGHLASFADPIVRCKKCKATHRADKIIV
                     EVSQIEIPESADLDEFDKTIVEKNIKCPKCNGDFENAKKFNMMFEVGIGPEGESAYLR
                     PETCQSIFVDFPRIFKTMRGKLPLGIAQTGKSFRNEIAPRQSLLRLREFYQAEIEV"
     gene            complement(684..1523)
                     /locus_tag="QGF01_02525"
     CDS             complement(684..1523)
                     /locus_tag="QGF01_02525"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HJN19807.1"
                     /translation="MQIGHHVSISGSLANAVTNANERECSAFQIFTGNPRGWYTKDIT
                     DEEALKFKNNLSQTNIDRFATVAHMPYLPNLSSPEPPVYEKSIKAMIREVKRCDQIGI
                     PFLVTHLGSHKGTGAEKGIKRLTSALIEVAEKTKNVTILLENTAGQKNSVGSNFSELS
                     EIFSSLKPADRFGVCIDTCHAFAAGYDLKHEKNVKSTFDEFDKEIGMEHIKILHLNDS
                     KGELGSNLDRHEHIGMGHIGSTGLGKVIKLMNKNKTPIILETPIDERRSDFEDIGAAK
                     ELA"
     gene            1622..2644
                     /locus_tag="QGF01_02530"
     CDS             1622..2644
                     /locus_tag="QGF01_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HJN19808.1"
                     /translation="MISFGHDEIVKYPFLAEAGQYLKDQGFTLEQFATDPDLQIIVDR
                     AYERIESASQGNIYNYKVDDSSNKDSSLPLEVFSFLIAIILLKLAGMNTLVSRFSLAE
                     ARRAERFLEKDLVGKSNKTNEEFATKIFLDLFSIKIKKNGDYFTIPIADYLKHAVHFH
                     ELEWKLINRHVENGMVFLTPHESVRLTRKQLGNYIGTRIRSAQTPESFQVFEEKVAKL
                     VKMGKKLTVTTTISTEFPPCIKRAIEVLNNGENLSHSGRFMLGTFLLGRGQTIEQIAP
                     LFKNAPDYKERTTLYQLKQLSGETGSNTKYACPSCDKIKSNDLCFAIPECDNIINPMQ
                     FGKKRL"
     gene            2645..3769
                     /locus_tag="QGF01_02535"
     CDS             2645..3769
                     /locus_tag="QGF01_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HJN19809.1"
                     /translation="MFEKDLKFLEDAFKQYYFDHFNLINVPDRASEREFGYMKFNTGM
                     IRHISLKTDKDLHLMLMSNIPSDVFCSNAYYTFPSMPMSEKDWKGADIIFDIDAKDLK
                     LPCRKDHTCNKCTSCGEIFVSQDGCPKCKSNKIEHISLPCDNCMAGLKKELSKLDKVL
                     TDDLNIPANDIVTSFSGNEGFHLYVKHSQYNDLGSKERGELIDYIKFQKVIPETFGFK
                     KNNPSKSSFPEFDESGWKGRVAKELFGSKSKKSKLVSKIISDGYVAYREKLDEIGKNS
                     IGIKIDPNVTVDIHRIFRLEGSLNSKSGLCKLACKDVGKFRPYTEACLIDDKPTEIIA
                     NCPIKFQLKNKKFGPYNGEKTSVPKYAAVYMMCKGIANSA"
     gene            3833..6685
                     /locus_tag="QGF01_02540"
     CDS             3833..6685
                     /locus_tag="QGF01_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HJN19810.1"
                     /translation="MYSSSKADNTPPPDAGKYIRVGIIAIIIIATFVVVGHQGIIMLM
                     NVEEFADLFTTPLYFSLISGLILAAIALVRVNVVRRASIGWYVLQTFLGFMSRQGTNA
                     SAQTIPLFGDFKISGIQFAIWQITKVLLFGAFFANLMFGFSVVYLVDGNDLGIDNIPT
                     IFSLPFVTPPTDHSYATEKVVPMIPALLVLVPPLLGAIGTRLLLYIGVHHVIKTVTAF
                     LHDSTEGKPKYLSYVSTLEAVIGIGVLWAGFNMFFGNQIDYNTKYAIGGTLVVGLALI
                     AFSFLDRMRSRILTHMLKRDVYIRIITIVVIALIVGIAMSVNNSVADAKKIEYLGPYN
                     AQQIGVNRYLGELDQIEEHIHDVTLKSISPNNISGYIDENKDVLDGIRVWDWQAAFAK
                     LKPEIGLIPYVTFEDNDILRFNDKLYWTASMAPILPSSVSLENRWYNEHLVYTHVPEG
                     FLTLEATDGSIVDSSELFPQRKIYYGEGGLLDTTWSGYPTNRDGSTAELNNETYTGMG
                     GLDISPPISWMFEPNFMISYPGDSIHVMRYKDVNERMSTLYPYFLFNLFGKELDSLPV
                     TDGKATYWLVPLIIGYDTSNIPWSVGNPYLRLAGYALIDTYDGDIQLIQHGDDFFTDM
                     FMTQYQDNVIEMPEWLEKQIRYPQELFNWKTEMYNIYHVTDVDTFIQANEFYEIPRGL
                     DTYYIEAKPPGFEEPEFVGLLSLELRGSQGRNLAGYMVVENDTPNLGKMQFYEVPIDS
                     DTKLLGPTSVREALDRDPDFAQLKTLLRNPRIGDNILYRVGEHDTYFIPVYTAGAGGV
                     VAQLGTIAAVGAAFTGEYYVGLGDTQESAFEAYLQKLSGVATTSSVTSGGVSMIMDKT
                     AREDAIMSIFEEKEIKITTPTSIQIPLSFSIGDIAFYSKADLESTTELITKFIGEAGV
                     GKRILMWEVDDTINFGYLKIVDNVTELHYIAIEVGK"
     gene            6685..7227
                     /locus_tag="QGF01_02545"
     CDS             6685..7227
                     /locus_tag="QGF01_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962336.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HJN19811.1"
                     /translation="MLLVVDNGSIYTKNLVNFLTKEETQFIDQKFDEVDLSDIGQFNS
                     FILSGRRKNNQKMNAINSQIIKYTVSEKKSLLGICYGAEILALTLGGTIRKSPKSVKG
                     EEIIVTEKENKLCEGKINAYESHNYEISRLGNYLQGLASSQSCKYEIIRHINHDIFGT
                     QFHPEMSDDGKSIIQNFLLV"
     gene            7262..7399
                     /locus_tag="QGF01_02550"
     CDS             7262..7399
                     /locus_tag="QGF01_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19812.1"
                     /translation="MVKKLEVQTNDKGEITSPTYLDLIAKINEVIEILSIEKVDDKDL
                     R"
     gene            complement(7411..7484)
                     /locus_tag="QGF01_02555"
     tRNA            complement(7411..7484)
                     /locus_tag="QGF01_02555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7448..7450),aa:Gly,seq:ccc)
BASE COUNT         2512 a         1309 c         1400 g         2437 t
ORIGIN      
        1 acttcaatct ctgcctgata aaactctctc agtctaagca gactttgtct aggcgctatc
       61 tcatttctga aacttttacc tgtttgtgca attcctagag gaagctttcc tctcattgtt
      121 ttgaatattc gtgggaaatc aacaaatatt gattgacatg tttcaggtct tagatatgca
      181 gattcacctt ctggtcctat tccaacttca aacatcatat tgaatttttt tgcattttca
      241 aaatcaccgt tacattttgg gcacttgata tttttttcta caatagtttt gtcaaattca
      301 tcaaggtcag cgctttcagg tatttctatt tgactaacct caacaataat tttatcagct
      361 ctgtgtgtcg ctttacattt tttgcatctt acaattgggt cagcaaaact tgcaagatga
      421 ccagatgcat caaacacaga cttggacatt atttgtgaac cgtcaatctc aatcatctta
      481 tcacgtctaa ctaattctct tctccaaagt tccaggaatt tattttttaa acttacacca
      541 gatggaccat attcccaaaa acctgcctga gcatcagcat agatctcaga acttggaaag
      601 taaaatcctc gctccaatgc aagcttcatc acatcatcat atgtcataat ttcaaactct
      661 cctggtagaa taaattctta atttcatgca agttccttag ccgctccaat atcttcaaag
      721 tcactccttc tttcatcaat tggtgtctct aatattatag gagtcttatt tttattcatt
      781 agtttaatca ctttacctaa tccagtactt ccaatatgtc ccataccaat atgttcatgt
      841 ctatcaagat tggatcctaa ttcgcctttt gaatcattca aatgtaggat tttgatatgc
      901 tccattccta tttctttgtc aaattcatca aaagtggatt tgacattttt ttcatgtttt
      961 aaatcatatc cagcagcaaa tgcatgacag gtatcaatac aaaccccaaa cctgtcagca
     1021 ggttttaggc tagaaaagat ttcagataac tctgaaaaat tagaacctac tgagtttttc
     1081 tggccagcag tattttctag tagaatggta acgtttttgg ttttttctgc aacttcgatc
     1141 agcgcacttg tcaacctctt gattcctttt tctgctcctg ttcctttatg gctacctagg
     1201 tgtgttacaa gaaatgggat gcctatttga tcacatcgtt tgacttctcg aatcattgct
     1261 ttgattgatt tttcataaac aggtggttct ggagaagaaa gatttggtag ataaggcatg
     1321 tgtgccacag tagcaaatcg atctatgtta gtttgactaa gattattctt gaatttaaga
     1381 gcctcctcat ctgtaatatc tttcgtatac catcccctag ggtttccagt aaagatctga
     1441 aatgcagaac attcacgttc atttgcattg gttaccgcat tagcaagcga acctgatatg
     1501 gatacatgat gaccaatttg cacgactaaa aaccctcatt acataattta aattagtata
     1561 acgtttttgc cttcaattct tttaaagtaa attttttaga aaggtcggag tatgtttgta
     1621 tatgattagt tttggacatg atgagattgt aaaatatcca tttttggcag aagctggcca
     1681 atatctaaaa gatcaaggtt ttacacttga acaatttgca actgatcccg atcttcagat
     1741 tattgttgat agggcatatg aaagaattga atctgcatca caaggaaata tttacaatta
     1801 caaagtggat gattcgtcta acaaggactc ttcacttcca ttagaagtat tttcattttt
     1861 gatagcaata attctattaa agttagctgg aatgaataca ctcgtcagtc gtttttccct
     1921 tgctgaagcc cggagggctg aaagattttt ggaaaaggat cttgtgggta aatcaaacaa
     1981 aacaaatgaa gaatttgcaa ctaaaatttt tctagatctt ttttccataa agataaaaaa
     2041 gaatggtgat tactttacaa ttccaattgc tgattacttg aaacatgctg tacattttca
     2101 tgagcttgaa tggaagctta ttaaccgtca tgtggagaac ggtatggttt ttttaactcc
     2161 tcatgaatct gtgaggttaa ctagaaaaca actaggaaat tatattggaa ctagaattag
     2221 atccgctcaa acaccagaat catttcaagt atttgaagag aaagtggcta aattagttaa
     2281 aatgggcaaa aaattaaccg tcactacaac catttcaacc gaatttccac catgtattaa
     2341 gcgcgcaatt gaagtactga ataacggaga aaatctatct cactctggta gatttatgct
     2401 aggtacattt cttcttggta gaggacagac aatagaacaa attgcaccat tattcaaaaa
     2461 cgctccagat tataaagaaa ggactacgtt atatcaactg aaacaacttt ccggtgaaac
     2521 aggtagcaac accaaatatg cctgcccatc atgtgacaag attaaaagta atgatctttg
     2581 ttttgcaata ccagaatgtg ataatattat aaatccaatg cagttcggaa agaaaagatt
     2641 gtaaatgttt gaaaaagatt taaaattttt ggaagacgct ttcaagcaat attattttga
     2701 tcatttcaat ttaatcaacg ttccagacag agcatctgaa agagaatttg gatacatgaa
     2761 atttaacact ggaatgatta gacacatttc gttaaagacg gataaggatc tacatttaat
     2821 gttaatgtca aatatccctt cagatgtttt ttgttcgaat gcatattaca cttttccaag
     2881 catgccaatg tcagagaaag attggaaagg agcagatatt atttttgata tagatgcaaa
     2941 ggatctaaaa ttaccctgta gaaaagatca cacttgtaac aaatgtacat cttgcggcga
     3001 gatatttgtg tcacaagatg gctgcccaaa atgtaaatca aataaaatag aacacatatc
     3061 actgccatgc gataactgta tggctggctt aaaaaaagaa ctttcaaaac tggacaaagt
     3121 tttgactgat gacctaaaca ttccagctaa cgacatagtg acttcttttt ctggaaacga
     3181 ggggtttcat ttgtatgtga agcattcaca gtataatgat ttaggatcaa aagaaagggg
     3241 tgaattgatt gattacatca agtttcaaaa ggtcatacct gaaacgtttg gttttaaaaa
     3301 aaataatcct tctaaatcat catttccaga gtttgatgaa tctggctgga aggggcgagt
     3361 agcaaaggaa ttatttggtt ccaaatcaaa aaaatccaaa ttagtttcaa aaattatttc
     3421 cgatggttat gtagcatata gagaaaaact tgatgagatt ggcaaaaact cgatcggcat
     3481 aaaaatagat ccaaatgtaa cagttgacat acacagaatc ttccgtcttg agggttcatt
     3541 aaacagtaaa agtggtcttt gtaagcttgc ctgtaaagac gttggaaagt ttaggcctta
     3601 cactgaggcg tgtctgattg atgataaacc gacagagatt attgcaaatt gtccaattaa
     3661 atttcaacta aaaaataaaa aatttgggcc atataacggc gaaaaaacat ctgttccaaa
     3721 gtatgcagct gtgtatatga tgtgtaaagg cattgcaaat tcagcataga atgataaaat
     3781 ttgggtattc gttgccaagg aattaatagc tcctgaatta aattttgtaa gtttgtatag
     3841 ttcatcaaag gcagataaca ctccaccacc agatgctggt aagtacatta gagtcggaat
     3901 tatcgcaatt atcataattg cgactttcgt ggttgttggt catcagggca tcatcatgtt
     3961 gatgaatgtg gaagaatttg ctgatctctt tactacgcca ctttactttt cattaatttc
     4021 cggtctgatt cttgctgcta tcgcattagt gcgtgtaaat gttgtaagac gggcttctat
     4081 cggatggtat gttttacaaa ctttccttgg gtttatgagt cgtcagggta ctaatgcctc
     4141 tgcacaaacc ataccacttt tcggcgattt taaaatttca ggaatacagt ttgctatttg
     4201 gcaaattacc aaggttttac tctttggcgc attctttgca aatcttatgt ttggattttc
     4261 tgttgtttat ctggtagatg gaaatgatct agggatagat aatataccta caatattttc
     4321 acttccattt gtcacaccac caactgatca ttcctatgca actgagaaag tagttccaat
     4381 gattccagct ctcttggttt tagttccacc gttacttgga gctattggta caagattact
     4441 gttgtatatt ggagttcatc acgtaatcaa aaccgttaca gcatttcttc atgattcaac
     4501 agaagggaag ccaaaatatc tcagctatgt atcaacactg gaagcagtta taggaattgg
     4561 tgtactttgg gctggattca atatgttctt tggtaaccaa attgattaca atacaaaata
     4621 tgcaataggt ggaacacttg ttgttggctt ggcgttaatt gcattttcat tccttgatag
     4681 aatgagatct agaatactta ctcatatgtt gaagagagat gtttacatta gaattatcac
     4741 tatagttgta attgcattaa ttgtaggcat agcaatgtct gttaacaata gtgttgctga
     4801 tgcaaaaaag atagaatatc ttggtccata taacgcacaa caaattggtg tgaatagata
     4861 tcttggagaa ttagatcaga tagaggaaca tattcacgac gttaccctca aatcaatttc
     4921 tccgaataat attagcgggt atatagatga gaacaaggat gtacttgatg gtatacgtgt
     4981 atgggactgg caagcagcat ttgccaaatt gaaaccagaa attggtctta taccgtatgt
     5041 aacttttgaa gataacgata ttttgagatt taatgataaa ctgtattgga ctgcatcaat
     5101 ggcaccaatc ttaccttcat ccgtcagttt ggaaaataga tggtataacg aacatttagt
     5161 atatactcat gttcctgagg gatttctaac attagaagca actgatggat caattgttga
     5221 cagttctgaa ctatttccac aaaggaaaat ctactatgga gaaggtggtt tacttgatac
     5281 aacatggtct ggatatccta caaacagaga tggaagtact gcagagttga ataatgaaac
     5341 ttatacagga atgggcggct tggacatatc tcctcctata agctggatgt ttgaacccaa
     5401 ctttatgatt tcatacccag gtgattctat ccacgttatg agatacaagg atgttaacga
     5461 aaggatgtct acactatacc cttatttctt attcaactta tttggaaagg aattggattc
     5521 acttccagta actgatggaa aagccactta ctggctcgtt ccactcatca ttggatatga
     5581 tacaagtaac atcccatggt cagtaggtaa tccatatcta agattagcag gatatgcatt
     5641 gatcgataca tacgacggag acattcagtt gattcaacac ggagacgact ttttcactga
     5701 catgtttatg acacaatacc aagacaacgt aatcgagatg cctgaatggt tagaaaaaca
     5761 gatcaggtat cctcaagaac ttttcaattg gaagactgaa atgtataata tttaccatgt
     5821 cactgatgtt gatacattca ttcaagcaaa tgaattctat gaaattcctc ggggtttaga
     5881 cacttactat attgaagcaa agccacctgg atttgaagaa ccagaatttg ttggcttgtt
     5941 gtcactcgaa cttcgtggtt cacaaggaag aaacttggcc ggatatatgg tagtagaaaa
     6001 tgatactcca aacttgggta aaatgcaatt ttatgaagtt ccaattgact cggacactaa
     6061 actattaggt cctacttctg ttcgtgaagc acttgacaga gatcctgatt ttgcgcaatt
     6121 aaagacgctt ctgagaaatc cacgaattgg tgataacata ttgtatagag taggagaaca
     6181 tgatacttac tttataccag tttatactgc tggtgctggt ggtgttgtag cacaacttgg
     6241 tactattgct gcagtaggtg ctgcatttac tggagaatat tatgttggat tgggcgatac
     6301 acaagaatcc gcatttgagg cttatctaca aaaattatca ggcgtagcaa ctacttcatc
     6361 tgtaaccagt ggtggggtca gtatgataat ggataagacc gcacgagaag atgcaatcat
     6421 gtcgatattt gaagaaaaag agatcaaaat aactactcca acatcgatcc agattccatt
     6481 atcatttagt ataggtgata ttgcattcta ttcaaaagct gacttggagt ctacaactga
     6541 attgattaca aagtttattg gtgaagctgg agttggtaaa cgaattctaa tgtgggaggt
     6601 agatgataca attaactttg gatatctcaa aattgttgat aacgtaactg aactacatta
     6661 tatcgcaatt gaggtaggca aatagtgcta cttgttgtgg ataatggttc tatctacaca
     6721 aaaaatttgg taaactttct tactaaagag gaaacccaat ttatcgacca aaaatttgat
     6781 gaagttgatc tatcggatat aggacaattc aattcgttta ttctttctgg acgaagaaaa
     6841 aataatcaaa aaatgaacgc cataaactct caaattataa aatatacagt atctgagaaa
     6901 aaatcgttac ttggaatttg ttatggggct gaaattttgg ctctcacatt gggtggaaca
     6961 ataaggaaat ctccgaaatc tgttaaaggt gaagaaatta ttgttactga aaaagaaaat
     7021 aaactatgtg aagggaaaat caatgcgtat gaaagtcata attatgaaat ctcacgatta
     7081 gggaattatc ttcaaggttt agctagttct caatcatgca agtatgaaat catacgacac
     7141 atcaatcatg atatttttgg tacacagttt catcctgaaa tgagcgatga tggtaaatct
     7201 ataatccaaa attttctcct agtctaatag attgaaatat agcaaataat tagaaaatat
     7261 aatggtaaaa aaattagagg tgcaaacaaa cgataaaggt gaaatcactt cgcctaccta
     7321 tctcgaccta atcgctaaaa tcaatgaagt aattgaaatt ctttcaattg aaaaagtgga
     7381 cgataaggac ttacgttaat ttcaatagta tgcggagtgc gggatttgaa cacgcgacct
     7441 ccagcttggg aagctgacgt cctgccagtc tggactaact ccgcttactg attttagtaa
     7501 gaatcttact aaaaacagta atcacatagc tgagattcat tctgatccag catacaagga
     7561 atggcttttt gaaaacaaaa acccactgta cgcaagatac atcttgcgtc gaagctcgcg
     7621 tgttgacaca ttattaatgc ttaatattac atgtgaga
//
LOCUS       DASCPJ010000057         9275 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000905075, whole genome shotgun sequence.
ACCESSION   DASCPJ010000057 DASCPJ010000000
VERSION     DASCPJ010000057.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000905075"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="QGF01_02560"
     CDS             <1..622
                     /locus_tag="QGF01_02560"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965681.1"
                     /note="catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Sulfolobus
                     solfataricus has two 5'-methylthioadenosine
                     phosphorylases; 5'-methylthioadenosine phosphorylase II
                     has a higher affinity for 5'-deoxy-5'-methylthioadenosine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HJN19813.1"
                     /translation="KKHTIPPHLINYRANIWAFKELGIKRIIAPSAVGSLKEEIKPEH
                     FVIPTQFLDFTKSRKTSFSEEGKVIHISVADPFCPELQSIVSNNMKNQNISMHENCTY
                     VCIEGPRFSTKAESKFYKSIGADVIGMTLVPECQLAREAQMCYTSISTVTDYDVWAEK
                     PVTAKEVINTLTKNVQITKKLLTELIDKIPKTRSCSCDKALSEAEF"
     gene            complement(619..903)
                     /locus_tag="QGF01_02565"
     CDS             complement(619..903)
                     /locus_tag="QGF01_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19814.1"
                     /translation="MNLKVHINNIDGTQMAAKINGVFELDDHSFDFTAIAFGRIGGQN
                     IGAKLSDDTKKTLSDLDYNIDEVIDELQRNLVKGNISIPDDVKRESFADD"
     gene            complement(910..1311)
                     /locus_tag="QGF01_02570"
     CDS             complement(910..1311)
                     /locus_tag="QGF01_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19815.1"
                     /translation="MGEIYRCCENPQITYLAPVNININERTIGSVDVWRCATCKKMFC
                     EEKQLGIDSLTEIVGMPKIEDDEKWAVIISKLQKGKDKWKLVKLKQNGIIKYETADEK
                     ILDLKIEDYKIVDDYHNSFLVEDHLNRAVEI"
     gene            complement(1318..1686)
                     /locus_tag="QGF01_02575"
     CDS             complement(1318..1686)
                     /locus_tag="QGF01_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19816.1"
                     /translation="MNYEKIKNDLITEVRLTKNQAEVFLLVVTEGKMPASQIAKLADL
                     TVDEATEISQKLVELGGFIDMPKTEYEAMHPRFTAVNMYRRMCERENIDFGKNLVVDN
                     IGIALEDVYDDARTKYNKKM"
     gene            1787..2248
                     /locus_tag="QGF01_02580"
     CDS             1787..2248
                     /locus_tag="QGF01_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HJN19817.1"
                     /translation="MSKFNRPAPYTDQKSLNFIIPIILLLGIGVVYVMTQKSDTSFAN
                     FILIGGAAITIIYWVPTLKKLVVENKPKYTAKEAEAKNWVYDLIRGDNELVFVAEVPG
                     PDDKIMVRLIDGILYIRGSGGFSKEVPIEGSQEMKITDFKYRNGVLTLRIH"
     gene            complement(2245..3342)
                     /locus_tag="QGF01_02585"
     CDS             complement(2245..3342)
                     /locus_tag="QGF01_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HJN19818.1"
                     /translation="MKKKPEKKTARDKQKTLVELLKKHGAQTYDDLNKGDFPKFSIPS
                     RSVSNIVYDKKLRQYILGTNSAVRNSRNTSQLRSFSQLMWLAFFANRLTKEKKSSTLR
                     DVYYSSQAFEIDFEDQSESDNIIVDLEAMLTRPREDFHVFPEERSSVFGDLTIEYTVP
                     GYEGKKMNLSDHPDGYAIGPSLTSAELLETSAEVIIAIEKGGLFTRFIEEQVDKKFKS
                     IIVDTGGQAPRSTRTLLKRLHDELSLPVVILTDGDVYGEHIAMVIKSGSANAAHLREL
                     TVPDAKWVGVWATDIEKYKLPTIPMTESDIKRCHDLKKDPRYQDGIWKKELEVFLKIK
                     RKAELEAFSKYGLTNITDKYLPQKLELAKSL"
     gene            complement(3326..5164)
                     /locus_tag="QGF01_02590"
     CDS             complement(3326..5164)
                     /locus_tag="QGF01_02590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HJN19819.1"
                     /translation="MSSKESFNQISPSEFFYRNRDLAGFSNPTRSLYTAVREFVENAL
                     DACDQRGIFPDVHLSIKAVDPDKPDPKQYILTVRDNGPGIESKHVPLAFGTVLYGSKF
                     GLKQARGMFGLGATMAILYGQITTNRPVTVKSSTDGKIQDQFEMLLDIQKNKPVILKN
                     QTKEVSKTGLSVSICLDGDYAKAGSKIRDYVYQTSLITPYATITFDDPKGEKFKYTRI
                     VKNMPSAPTIIRPHPYGIDVETIRRMLLDTHYQIPTVDDSMIVKVRKELGLSKKNLNY
                     SVIMQKAQKKWKVLTRPVRIVVAMMSFLKMDFEKLSKITIEEIDIGNKKLTYWDFGES
                     QSISIDMDPESHYYKQLANTVQGETLTSFLTKRFQRVGPTTASKFAEFAKFKPEHRIG
                     NMNNQELVKLTDALQSYDDFMAPDPSCLAPLGATALEKGMQRFFEPDFLAVVQRGASA
                     YSGFPFIIEMGIAYGGKISSHGIKVYRFANRIPLLYDEGSDVVLKVVNDTDWSRYKIK
                     GDPPLVIVSHICSTRVPYKTVGKENVADRPELERELKLALLSLSRKLSSFMSKKGQAE
                     AAKKRANLYTKYIPLIAQFCTELAGKTKQPNYKKMITEVPVVEEKT"
     gene            complement(5154..5723)
                     /locus_tag="QGF01_02595"
     CDS             complement(5154..5723)
                     /locus_tag="QGF01_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HJN19820.1"
                     /translation="MSFEKIIRIPGDRIGVLIGKAGKTKIEIEESCSVKLEIDSNTGE
                     VTLRGSGDVTTIQPFKAVEIVTAIGRGFSPINAMKLLEEPNALHVINLQEFAGKSPDQ
                     MERIKGRIIGEGGKARTNMENLSNTSISVYGKTVSVIGSPNQLKIVIDALSSLSSGSM
                     HGSVYNKLETARRREKIDKLQLWENQDVF"
     gene            complement(5720..6505)
                     /locus_tag="QGF01_02600"
     CDS             complement(5720..6505)
                     /locus_tag="QGF01_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HJN19821.1"
                     /translation="MKTFSDKTDDLDFFLNSKNFLKSKKKTLPDGFKKNKTVNEVLDK
                     LTTLTLFNLIKSKTIAYVNGVVRAGKESVVFWAVSPDNNDVALKVYLVTTASFKKRAT
                     YILGDPRFSRIKKGTRNLVYLWAKKEYQNLTRCVRNSIPVVKPITVKNNVLALEFVGK
                     DGIPAKTLIESEVNGDDYKSAIRLIKQLYKKARLVHGDFSEYNIFKTENGLVLFDLGS
                     AVDLEHPKAHEFLKRDIENISNFFVKRGLTVKNPIDIINEVTK"
     gene            complement(6529..6840)
                     /locus_tag="QGF01_02605"
     CDS             complement(6529..6840)
                     /locus_tag="QGF01_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HJN19822.1"
                     /translation="MGKRKVLNESALKEIKVAQEGELFGRVIKLLGSDQVMVKCTDNI
                     TRRGRIRGKLKRRIWIRDNDIVIIAPWDFKEQERGDIIWRFTLSQVEWLKDNGHIPRD
                     F"
     gene            complement(6878..7171)
                     /locus_tag="QGF01_02610"
     CDS             complement(6878..7171)
                     /locus_tag="QGF01_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HJN19823.1"
                     /translation="MFSARLVNQQNTSMLNICDYELLGKTITNHGLKIEINQAYYGEK
                     KIDDVEAKYLLKKSTSVNMVGKQIISLSVSLGIGTLNGVKEIDGIPFLIVFKM"
     gene            complement(7171..7593)
                     /locus_tag="QGF01_02615"
     CDS             complement(7171..7593)
                     /locus_tag="QGF01_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HJN19824.1"
                     /translation="MKTSEYEKLLKRIDVKDPKISDDTDSRFVVPPVDVMWEGQRTIL
                     RNFSDYPKIMRRDPSKVLQYLSKEFAVPAEKIGDTAMFIGKREPDDFTRLFKIYVTDY
                     IECQTCKSPDTKIVKEKRISFLLCEACGAKSTVKGKYA"
     gene            complement(7646..7789)
                     /locus_tag="QGF01_02620"
     CDS             complement(7646..7789)
                     /locus_tag="QGF01_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HJN19825.1"
                     /translation="MTTAYCVKCREKREIKNPQITELKNGRPAVKGTCPECGTNVFRI
                     GKP"
     gene            7950..8486
                     /locus_tag="QGF01_02625"
     CDS             7950..8486
                     /locus_tag="QGF01_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19826.1"
                     /translation="MASKKGLGITIAILVGVVAASFSVYLIPENMDTEMKFVVSDFEK
                     YLDDVDEKTSMLSTGVEESFGDLMNHELSPEEYFVTANITQQQVNSLIIELTLSGEPQ
                     EWTASYKTYVGALKKLNEQIRETVIVANLMNEINSIDCDEECIDSMDRRLNELIPKVY
                     ELRAESLDLIEKSNNFRP"
     gene            8562..9125
                     /locus_tag="QGF01_02630"
                     /pseudo
     CDS             8562..9125
                     /locus_tag="QGF01_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
BASE COUNT         2909 a         1652 c         1406 g         3308 t
ORIGIN      
        1 aaaaaaacac accattccac cacacctaat aaattaccgt gcaaatattt gggcctttaa
       61 agagttagga attaaaagaa ttattgcgcc ctcagctgta ggaagtttaa aggaagaaat
      121 aaaaccagaa cactttgtta tcccgacaca atttttagat ttcacaaaat ctcgaaaaac
      181 ctcattttcg gaagaaggta aagtaattca tatttcagtt gcagatccat tctgcccaga
      241 attacagagt atcgtttcta ataatatgaa aaatcagaat atatccatgc atgaaaattg
      301 tacgtatgtt tgcattgaag gtccaagatt ttctactaaa gccgaatcaa aattctacaa
      361 atctatcggt gcagatgtga ttgggatgac tctagtgcct gaatgtcaac ttgcaagaga
      421 agcgcagatg tgttatacgt caatttctac tgtcacagat tatgacgttt gggcggaaaa
      481 acctgtaacc gctaaagaag taataaatac attaacaaaa aatgttcaaa ttacaaaaaa
      541 acttctcaca gaactaattg ataaaatccc aaaaactaga tcatgttcat gtgataaggc
      601 attaagtgaa gcagaattct aatcatctgc aaatgattca cgctttacat catcaggaat
      661 actgatattt ccttttacta aatttctttg taattcatca ataacttcat caatattata
      721 atccaaatca cttaatgttt tttttgtatc atcagataat ttagcaccaa tattctgacc
      781 accaatcctg ccaaaagcaa ttgcagtaaa atcaaatgaa tgatcatcaa gttcgaacac
      841 tccattaatt tttgcagcca tttgcgtacc atcaatattg tttatgtgta cttttaggtt
      901 caagtggatc taaatttcca ctgccctgtt caaatggtct tctacaagaa aactattatg
      961 atagtcatca actatcttgt aatcctcaat ttttagatct aaaatttttt cgtcagctgt
     1021 ttcatatttt ataattccat tttgttttaa ttttacaagt ttccacttat cttttccctt
     1081 ttgaagttta cttataatca cagcccattt ttcatcatcc tcaatttttg gcataccaac
     1141 aatttctgtc agtgaatcta ttcccaattg tttttcttca caaaacattt ttttacatgt
     1201 agcacaacgc caaacatcta cactaccgat agttctttca ttgatgttta tgttaactgg
     1261 ggctaggtaa gtaatttgag gattttcaca gcatctgtat atttcaccca tacaaactca
     1321 catttttttg ttatatttag tccttgcatc atcatataca tcttccaatg caataccaat
     1381 attatcaaca actaaatttt ttccaaaatc aatattttct cgttcacaca ttcttctgta
     1441 catatttact gcagtaaacc tcggatgcat tgcctcatat tcagttttag gcatgtctat
     1501 aaaaccacct aattcaacaa gtttttgaga tatttctgtg gcctcatcaa cagttagatc
     1561 tgcaagtttt gcaatttggc tagccggcat ttttccttca gtaacaacta gtaaaaacac
     1621 ttcagcctga tttttggtga gtctgacctc ggttatcaga tcatttttta ttttttcata
     1681 attcatcaat atttactctc aaataattac attaaaacaa tactaaaatt ggttgtttct
     1741 aaatttcaaa tatactttat atttggtaaa ataattttag aattaattgt caaaatttaa
     1801 cagacctgct ccatacacag atcaaaagtc cctaaatttc ataattccaa ttatactatt
     1861 actgggtatt ggggtagtct atgtaatgac tcaaaaatct gatactagct ttgcaaattt
     1921 tatcttgatt ggaggtgctg caattactat aatctattgg gttcctactc ttaaaaaact
     1981 ggtagtggaa aataagccaa aatatacagc aaaagaagca gaagctaaga attgggttta
     2041 tgatttaatt cgtggtgata atgaattggt ttttgttgca gaagtaccag gtcctgatga
     2101 caagattatg gttagattaa ttgatggtat tttatatatc aggggatctg gtggcttttc
     2161 gaaggaggta cctattgaag gctcacagga aatgaagatt actgatttta aatatagaaa
     2221 tggtgtgtta acacttagaa tacattaaag actttttgca agttcaagtt tttgtggaag
     2281 atatttatcc gtaatatttg tcaaaccata ttttgagaat gcttccaatt ctgcttttct
     2341 tttaattttt aagaacactt caagttcttt tttccaaatt ccatcttgat atcttggatc
     2401 tttcttgaga tcatgacatc gctttatgtc agattctgtc attggtatag taggaagttt
     2461 atatttttca atgtcagtag cccaaacacc aacccacttt gcatcaggaa cagttaattc
     2521 tcttagatga gctgcatttg ctgatcctga ttttattacc attgctatat gctcaccata
     2581 tacatcacca tctgtcaaaa taacaactgg taaacttagc tcatcatgca gtctttttaa
     2641 aagagttcga gttgaacgtg gagcctggcc accagtatct acaataattg atttgaattt
     2701 tttatccact tgctcttcaa tgaatcttgt aaataaacca cctttctcaa ttgcgattat
     2761 cacttcagca ctagtttcca gcaattcagc actagtcaga cttgggccta ttgcataacc
     2821 gtccggatga tcagacaaat tcattttttt tccttcgtat cctggtacag tatactctat
     2881 tgtaagatca ccaaatacac tacttcgttc ttcaggaaaa acatgaaaat cctctcttgg
     2941 tcgtgtaagc atggcctcta gatcaactat tatgttatca gattctgact gatcctcgaa
     3001 gtcaatttca aatgcttgag aggaatagta tacgtccctt aaggtggatg attttttttc
     3061 ttttgtaagt ctattagcaa aaaaagccaa ccacattaat tgtgaaaatg aacgaagttg
     3121 agatgtgttc ctggaatttc gtacagctga atttgttcct agtatatact gccgtaattt
     3181 tttatcatag acaatattac ttacagaacg gctgggaata gaaaattttg gaaaatctcc
     3241 tttatttaga tcatcataag tttgtgcacc atgttttttt aaaagttcaa ctagtgtttt
     3301 ttgcttatca cgtgcagttt ttttctcagg ttttttcttc aacaacaggt acctccgtaa
     3361 tcattttctt ataatttggt tgcttggttt ttccagctaa ttctgtacag aactgcgcta
     3421 tcaaaggaat atattttgtg taaagattag ctcttttctt tgccgcttct gcttggcctt
     3481 ttttagacat gaatgaagac aatttcctag atagtgatag taatgcaagt ttcaattcac
     3541 gttctaattc aggtctatct gctacatttt cttttcctac tgtcttgtat ggaactctcg
     3601 tggaacaaat gtgggatact atgacaagag gtggatcacc ctttatttta taacgtgacc
     3661 aatcagtgtc atttacaact tttagtacca catcacttcc ttcatcataa agaagaggaa
     3721 ttctgttagc aaatcgataa accttgattc catgactaga aatttttcct ccatatgcaa
     3781 ttcccatttc tatgataaat gggaatcctg agtatgcaga agcaccacgt tgtacaacag
     3841 caagaaagtc tggttcaaaa aaccgttgca tgcctttttc taatgcagtt gcgccaagtg
     3901 gagctaagca acttggatca ggtgccataa aatcatcata ggattgtaat gcgtcagtga
     3961 gttttacaag ttcttgatta ttcatgttac caattctgtg ttcaggtttg aactttgcaa
     4021 attcagcgaa ttttgaagcc gtggtaggtc ctaccctttg gaaacgtttt gtcaaaaagg
     4081 aagttagtgt ttcaccctgc acagtgtttg ctaactgctt gtagtaatga ctttcaggat
     4141 ccatatcaat agaaattgat tgtgattcac caaaatccca ataggttaat tttttatttc
     4201 ctatatcaat ttcttcaatt gtaatcttac ttaatttttc aaaatccatt tttaaaaatg
     4261 acatcatggc aaccactata cgaactggac gtgttagcac cttccatttt ttttgtgctt
     4321 tttgcattat tactgagtaa tttagatttt ttttagataa tcctaattcc tttctgactt
     4381 ttacaatcat actatcatct acagttggaa tttgataatg agtgtccaac agcatacgcc
     4441 taattgtttc aacatctatt ccatatggat gaggtctgat aattgttggc gctgacggca
     4501 tatttttaac aattcttgta tatttgaact tctcaccttt tggatcatca aaagtgattg
     4561 ttgcatatgg tgttatcaat gatgtttggt agacataatc acgtattttt gagccggctt
     4621 ttgcataatc accgtctaaa catatactaa cagataatcc agtttttgaa acttcttttg
     4681 tttggttttt tagtatgaca ggcttgttct tttgtatatc caaaagcatt tcaaattgat
     4741 cctgaatttt tccatcagta ctacttttca cggtaacagg tcgatttgtt gtaatttgac
     4801 catacaaaat tgccattgta gcacccaagc caaacattcc tctggcttgc ttcaaaccaa
     4861 attttgaccc atacaaaact gttccaaatg ccaaaggaac atgtttagat tcaattccag
     4921 gaccgttatc tcttacagtc aagatgtatt gttttggatc tggcttatct ggatcaactg
     4981 cttttattga caaatggaca tctggaaaaa ttcctctttg atcacatgca tcaagagcgt
     5041 tttcaacaaa ttcacgtaca gcagtataaa gtgatctagt tggattacta aagccagcaa
     5101 gatctctatt acgataaaaa aactcacttg gagaaatctg attaaaagat tctttagaag
     5161 acatcttgat tttcccacaa ttgtaattta tctatctttt cacgtcttcg tgctgtttcc
     5221 aatttattat aaacagatcc gtgcatacta ccactagaaa gagatgaaag tgcatctata
     5281 acaattttta attggtttgg tgaaccaatt acagaaacag tttttccata aacagaaatt
     5341 gatgtattgc taagattttc catatttgtt cgtgctttac ctccttctcc aataattctt
     5401 ccttttatcc gttccatttg atcgggtgat tttccagcaa attcttgcag atttatcaca
     5461 tgtaatgcat taggctcttc taataatttc atagcattaa ttggagagaa accacggcca
     5521 attgctgtaa caatttctac agccttaaat ggttgaatag ttgtaacatc cccggatcca
     5581 cgtaaagtaa cctcacctgt attactatca atctctagtt tcactgaaca tgattcttcg
     5641 atttcaattt tggtttttcc agctttacca attaatacac cgattctatc tccaggtatt
     5701 ctgattattt tttcaaaact cattttgtaa cctcattaat tatgtcaata ggatttttta
     5761 ctgtcaatcc tctttttaca aaaaaattag atatattctc aatatctctt tttagaaatt
     5821 cgtgcgcttt tggatgttct agatcaaccg ctgatcccaa atcaaataaa actaaaccat
     5881 tttctgtctt aaatatatta tattctgaaa aatcaccatg aaccaatcta gcttttttgt
     5941 aaagttgttt aatcaatctg attgcagact tgtaatcatc cccattaact tctgactcga
     6001 taagagtttt tgctggaatg ccatctttcc cgacaaactc caaggctaac acattatttt
     6061 tgactgttat tggtttcact actggaatac tatttcgaac acagcgagtt aagttttgat
     6121 attctttttt agcccataaa tataccaaat ttctagtacc tttttttatt ctagaaaacc
     6181 gtggatcgcc caaaatgtaa gtcgcacgtt ttttaaaact agctgttgta acgagataaa
     6241 ccttcaatgc tacatcatta ttatctggtg aaaccgccca aaataccact gattcctttc
     6301 ctgcacgaac tacaccatta acataagcaa ttgttttaga ttttattaaa ttaaaaagtg
     6361 ttaaagtggt taatttatct aatacttcat taacagtctt gtttttttta aatccatcag
     6421 gcaaagtttt tttttttgat tttaaaaaat ttttagagtt aagaaaaaaa tccaagtcgt
     6481 cagttttatc agaaaatgtt ttcatttaat caacgaaaat aaatttatct agaaatctct
     6541 tggaatatgc ccgttatctt ttagccattc tacttgactt agagtgaatc tccatattat
     6601 atctcctctc tcttgttctt taaaatccca tggtgcgatg atcacaatat cattgtcacg
     6661 aatccaaatt cgtcgtttta gttttcccct aattctacct cgtcttgtta tattatcagt
     6721 acacttaacc atcacctgat cacttccaag taatttgatt actctaccaa ataactcacc
     6781 ttcttgtgca actttaattt cctttaatgc actttcgttc agaaccttac gctttcccat
     6841 agaaaaaaat aaaaaatttt ctatataatt gtggtgatta cattttaaag acaattaaga
     6901 atggaatacc atcaatttct ttaacaccgt tcaatgttcc tatacctaaa ctcactgata
     6961 aagagattat ttgttttcct accatgttaa cactagtaga ttttttcaga agatattttg
     7021 cttcaacatc atcaattttt ttttctccat aatatgcttg attgatttca attttcaaac
     7081 catggtttgt gatagtttta cccaatagtt cataatcaca aatatttagc atagacgtgt
     7141 tttgctgatt aactagcctt gctgaaaaca ttatgcatat ttacccttga ctgttgattt
     7201 tgctccacag gcttcacaca gtaggaaaga aattcgcttt tctttcacta ttttagtatc
     7261 agggctttta caagtttgac attctatata atcagtcaca taaattttga aaagacgtgt
     7321 gaaatcatct ggctctcttt tgccaataaa cattgcagta tcacctatct tctcagcggg
     7381 aactgcaaat tcttttgata gatactgcaa aacctttgaa ggatctctac gcattatttt
     7441 tgggtaatca gaaaaatttc ttaaaatggt tctttgtccc tcccacataa catcaactgg
     7501 tggaaccaca aatcttgaat ctgtatcatc tgatatcttt ggatctttta cgtcaatacg
     7561 ttttagaagt ttttcgtatt ctgatgtttt catagaataa tcaaaataaa actacctata
     7621 tgggtttaga tataaaaaaa gtgttctaag gtttacctat tcggaaaaca ttagttccac
     7681 attctggaca tgtacctttt actgctggtc ggccattttt taattcagtt atctgtggat
     7741 tttttatttc tctcttttct ctgcatttta cacaatatgc tgttgtcatt ataacatacc
     7801 gcaatcaatg tcatatttag aactgtgatg taaaaaaatt ttaactagag gatagatgat
     7861 tatagggatt ttcaccaaat tatccgatat tatttaacta atttcattga aaaattaatt
     7921 tatggataaa gatctctgaa taacatcaaa tggcgtcaaa aaagggtctt ggaataacta
     7981 tagctattct tgttggtgtt gtagctgcga gtttttcagt ttatttgatt ccagaaaata
     8041 tggatacaga aatgaagttt gttgtatctg atttcgaaaa atatctagat gacgtagatg
     8101 aaaaaacctc aatgctgtcg actggtgttg aagaatcatt tggagacttg atgaatcatg
     8161 agttatctcc agaagaatat tttgtcactg caaacattac acaacaacaa gtaaattcat
     8221 tgataatcga actaacttta agtggtgaac cgcaagaatg gaccgcaagt tacaaaactt
     8281 atgttggtgc attaaaaaaa ttgaatgagc aaattagaga aactgttatt gttgcaaatt
     8341 tgatgaatga aattaatagt attgattgtg acgaagagtg tatagactcg atggatagac
     8401 gattaaatga attgattcct aaagtttatg aattaagggc agaatcacta gatcttattg
     8461 aaaaatcaaa taatttcaga ccataaacta gtcacatatc ctaaatctcc taatcataaa
     8521 agatgggaat cattaaaaac taattttgct atgtataatg tatgtcactc aacggtaaac
     8581 taaagaaaga tatcgaggca gcagttgcca ctgatttact agttgtttgt gttgatagag
     8641 acgatgatgt agggaaaaaa gctggaatta caactcctgt gattggtaga gatgcatgta
     8701 ttgaggctgc acaaagatta gcattagaag atcctgagga tgctgattca aacgcaatat
     8761 tttctgcgat aaaaacatat gaagatttaa tcagcaaagg atacaaagct caagtagtca
     8821 cggtggctgg ggtagaaagc agaggtgtgc aaggtgatga aaaaattgct gcagaaataa
     8881 aattagtatt aaagaaattt tctgctgacg gagctgtgat tgtatctgat ggagaagacg
     8941 atgaaatggt cattccaatc attcaaaata taattccagt tgtatctgtg caaagagttg
     9001 ttatgaaagt tagtagaaca attgaacatt cctatgcagt ttttggtaaa tttctaaaaa
     9061 tggttgtata tgatccaaaa tattcaaaat tctttttggg tgttccagga attttattat
     9121 taatagactg gcacataaat atgcatcatt tttatcttta ccttgtatta cagctgttat
     9181 ttttgtgttt tctgggtatt caaattgttt ccaatattta gcttgagctg ccatcatatg
     9241 tccttgcatc caaacatcat caccatcacc attcc
//
LOCUS       DASCPJ010000058         4907 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000915995, whole genome shotgun sequence.
ACCESSION   DASCPJ010000058 DASCPJ010000000
VERSION     DASCPJ010000058.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000915995"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            113..985
                     /locus_tag="QGF01_02635"
     CDS             113..985
                     /locus_tag="QGF01_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19827.1"
                     /translation="MASKLKNSLKQFTPPIVANKTKDLINYINFLKYKDIVKNNAELK
                     DKHKGRRCFLLGSGPSIKDENLKPLKNEIVFALNNFYVHDDFSEIMSGNIEKYYLTAP
                     IHPPQTETEWKDWFSDMEQHMPKNVNLIFGISNQNNNIKSILSHHNLFVNFKKYWYYA
                     GININDYYNYKPRDIDITRMTWIADTISIYSIIVAIYMGFSEIYLLGMDHNYICNNKS
                     NYRFYKDGIHQNDEDERMLKDTSLTKHLSFSIYKIFHQYELLSNNSDTEIYNTSRNSL
                     LDIFEYVTFKDIIK"
     gene            1048..1482
                     /locus_tag="QGF01_02640"
     CDS             1048..1482
                     /locus_tag="QGF01_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19828.1"
                     /translation="MINTIENKGKTLAIILRTTYNEKGINFFTPNEFSQQLAYMNHPS
                     GYEIQPHVHNAVQREVQFTKEVLFIKSGKVRVDFYDNEKNYLESRILASGDVILLAFG
                     GHGFEMIEDSEIIEVKQGPYAGESDKTRFKAISTGQILIKEE"
     gene            1483..2607
                     /locus_tag="QGF01_02645"
     CDS             1483..2607
                     /locus_tag="QGF01_02645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HJN19829.1"
                     /translation="MTTYPFIPVNSPLLDGNEKKYLNECIDTGWISSEGQFVNQFEDQ
                     FAKRLGRNHAIAVTSGTAALDVAIEALLIGPGDEVILPAFTIISCISQIIRSGAIPVL
                     VDSDPLTWNMNLQQIEEKITERTKAILVVHIFGLPVDLDPILELAKKNDIKIIEDAAE
                     MHGQTYNGNPCGSFGDISTFSFYPNKHITTGEGGMIVTNDAQLAEDCRSLRNLCFQPE
                     KRFVHERFGWNYRMTNIQAALGLAQLERLDEFVERKRIMGKKYTQGLRVLNEVQLPLE
                     KTDYAENIYWVFGVVLDDSIGFDAEEAMKMLEEKGIGCRPFFCPMNQQPVLRKMGFFI
                     DESYPVAERLYKQGFYIPSGVALNSEQINHVTNKLIEVLK"
     gene            2610..3365
                     /locus_tag="QGF01_02650"
     CDS             2610..3365
                     /locus_tag="QGF01_02650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJN19830.1"
                     /translation="MVFNAYAAYYDLLYQDKNYHKEAQYIYRFLEENGISSGKILELG
                     SGTGKHAEEFAKMGFCVHGIDLSQKMVQEANHRNNNNQKGQLFFEVGDVRNFEAGKKF
                     DAVISLFHVISYQTKNEDIQKMFKTAAKHLKQNGVFIFDFWYGPGVLTDPPAVRQKHL
                     ENKDIEVLRIAEPVMYPNENIVDVNYCVHIKQKRSGKVTELNETHKMRYFFIPEILQF
                     SSTWFTLKTNFAWMKDSKPGFNDWLSVCSLIKK"
     gene            3362..4549
                     /locus_tag="QGF01_02655"
     CDS             3362..4549
                     /locus_tag="QGF01_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012744.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HJN19831.1"
                     /translation="MKIIAIIEKPITSGGGFNQALNAIEQMNRISVDKFDFSVVTPTK
                     ENIYSLKKLNIKTSYVGITIFDKILSRLFTGPIWGQILAKLKIVGPFEKQLIKQNCDL
                     VYFVEQSSRAESIQNLNFIETVHDLCHQDQLEFPEVRNFGKFIRRERVFQNTLKRAFI
                     VMVDSEELAVKINLRYGVDRDRIVNMPFSPSPFLNETKASDSADILKLYKLDYGYFFY
                     PAQFWAHKNHYRIIEAVKLLHQEGKQCRVVFVGGDKGNYDFLDRLIRGYGLGKHVQSL
                     GFLPAEHMRALYENCQAVIMPTYFGSTNLPPLEAWLMGKPLIYSKLCSEQAGNAALLI
                     DPDDTNSVADAMKSMLDESISKKYKKNGYERLKQISTMRKISESNLSAKLLQFEQKMK
                     CWE"
     gene            4628..>4907
                     /locus_tag="QGF01_02660"
     CDS             4628..>4907
                     /locus_tag="QGF01_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HJN19832.1"
                     /translation="MLHKTGFELRRFSVEHSENARFISMLRANNVNLIFDVGANTGQF
                     GVLMREIGFDGKILSFEPLSEARESLLKISRNDSMWQVALQSAIGEENG"
BASE COUNT         1748 a          717 c          938 g         1504 t
ORIGIN      
        1 cctatgaatg ccataactaa cataaaaaat atcgaaggta taactcactt atctaaatcc
       61 ggaggagatg gagctgtacg agagtttgta gaattattat taaatgaata acatggcgtc
      121 aaaattaaag aatagtctaa aacaattcac accaccaata gttgcaaata aaactaaaga
      181 tctaataaat tacattaatt ttctaaaata caaagacatc gttaaaaata atgccgaact
      241 aaaggacaag cataaaggtc gacgatgttt tttgttaggt agtggtccat ctataaaaga
      301 cgaaaatcta aagcccttga aaaacgaaat cgtctttgca ttaaataact tctacgttca
      361 tgatgatttt tcagaaataa tgagtggtaa tattgaaaag tactatttga cagcaccaat
      421 acaccctcca caaactgaga ctgaatggaa ggactggttt tctgatatgg aacagcacat
      481 gccaaaaaat gtaaacttga tttttggaat cagtaatcaa aataacaata ttaaaagcat
      541 cctgtctcat cataatttat ttgtgaattt caagaaatac tggtactacg cagggattaa
      601 tatcaacgat tattataatt ataaaccaag agatattgat attactagaa tgacttggat
      661 agctgatact attagcattt attctataat tgttgcgata tatatgggat tcagtgaaat
      721 atatttactg ggaatggatc acaactatat ttgtaataac aaaagtaatt ataggtttta
      781 taaagacgga attcatcaaa acgacgaaga tgaaagaatg ctaaaagata cttcactgac
      841 taaacatctt tcttttagta tatataaaat ttttcatcaa tatgaattat tatcaaataa
      901 ttcagatact gagatctaca atacatcgag aaactcattg ctggatattt ttgaatatgt
      961 aacattcaaa gacatcataa aataattatt gttaagctct atggaagtaa taaaattatt
     1021 caaaataatt tactagaaat aatctttatg ataaacacaa ttgaaaataa gggaaaaact
     1081 ttagcaataa tcttacgaac aacttataac gagaaaggga taaatttctt tactccaaat
     1141 gaattctcac aacaactcgc atatatgaac cacccatccg gttatgaaat ccaaccgcat
     1201 gtgcataacg cagtacaaag agaggttcag ttcaccaaag aggtcttatt tattaaatca
     1261 ggaaaggttc gagtggactt ttatgataat gagaaaaact atctcgaaag tagaatttta
     1321 gcaagtggcg atgtgatttt acttgcattt ggagggcatg gttttgaaat gatagaagat
     1381 agtgaaatta ttgaggtaaa acaagggcct tatgcgggtg aatctgacaa aactagattt
     1441 aaagccatct caactggtca aatattgatt aaggaggaat aattgactac ttatcctttc
     1501 atcccagtca attcaccttt acttgacgga aacgaaaaaa agtatcttaa tgagtgtatt
     1561 gatactggct ggatatcttc tgaaggtcaa tttgttaatc aatttgaaga tcagtttgca
     1621 aaacgtttag gtcgaaatca tgccattgca gtgaccagtg gaacagcagc tttggacgtg
     1681 gctattgaag cattgctaat tggtccggga gatgaggtaa ttcttcccgc atttacgatt
     1741 atctcttgca ttagccaaat tattcgttct ggtgcaattc cagttttagt ggatagcgat
     1801 cccttgactt ggaatatgaa cctgcaacaa attgaagaaa aaatcactga acgcacaaaa
     1861 gccattttgg tggttcatat ttttggacta ccagttgatt tagacccaat actggaacta
     1921 gcaaaaaaaa atgacattaa aattattgaa gatgctgctg aaatgcatgg gcaaacttat
     1981 aatgggaacc cgtgtggaag tttcggagat atcagtactt ttagttttta cccaaacaaa
     2041 cacattacca ccggtgaagg gggtatgatt gtaaccaatg atgcacaact ggcagaagat
     2101 tgtcgtagtt tacgcaattt atgttttcaa cctgaaaaac gttttgtcca tgaaaggttc
     2161 ggctggaatt atcgaatgac aaatattcaa gctgcactag ggttagctca gttagaacgg
     2221 ttagatgaat ttgtggaaag aaaaaggatt atgggtaaga aatacacgca aggattgaga
     2281 gtattgaatg aggtacaact tccgcttgaa aaaacagatt atgcagagaa tatctattgg
     2341 gtttttggcg tggtattaga tgattctata ggttttgatg ctgaagaagc aatgaagatg
     2401 ttggaggaaa aaggaatagg ttgtcgtcca tttttttgcc caatgaatca acagcctgtt
     2461 ttgcggaaaa tgggattctt tatagacgaa tcttatccag tagcagaaag attgtacaaa
     2521 caaggtttct atattcctag cggtgtagct ttgaattctg aacaaataaa tcatgtaaca
     2581 aataaattaa ttgaggtctt gaaatgaaca tggtgttcaa tgcctatgca gcgtattatg
     2641 acttactgta tcaagataaa aattatcata aagaggccca gtatatatat cgattcctag
     2701 aggaaaatgg aataagtagt ggaaaaatat tagaactcgg ttctggaacg gggaaacatg
     2761 ccgaagaatt tgcaaaaatg ggattttgcg tgcacggaat tgatttaagt cagaagatgg
     2821 tccaagaagc aaatcataga aataataata accaaaaagg tcagttgttc tttgaagtcg
     2881 gagatgtgcg aaattttgaa gctggaaaaa agtttgatgc tgttatttcc ctttttcatg
     2941 tgattagtta tcaaacaaaa aacgaagata tacagaaaat gtttaaaaca gctgcaaaac
     3001 accttaagca aaatggcgtt tttatttttg atttctggta tgggccaggt gttttgactg
     3061 acccacctgc tgtaaggcag aagcatttag aaaataaaga cattgaagtt ttaaggattg
     3121 ctgagcctgt gatgtatcca aacgaaaata ttgttgatgt aaattattgt gtgcatataa
     3181 aacaaaaaag aagtggaaaa gttactgaat tgaatgaaac tcataaaatg cgttattttt
     3241 ttattccaga aattttacaa tttagtagca cgtggtttac tctgaaaaca aattttgcat
     3301 ggatgaagga tagtaaacct ggctttaatg attggcttag tgtctgtagc ttgataaaaa
     3361 aatgaaaatt attgctatta ttgaaaaacc aatcacatct ggtggtggtt ttaatcaggc
     3421 attaaatgct attgaacaga tgaacagaat atctgttgat aaatttgatt tttctgttgt
     3481 tactccaacc aaagaaaata tctattcctt aaaaaagcta aacattaaaa caagttatgt
     3541 gggaataacc atatttgaca aaatactgtc aagattgttt actgggccca tctggggaca
     3601 gattctagca aagctaaaaa ttgtaggacc atttgaaaaa caattaatta agcaaaattg
     3661 cgatttagtt tattttgttg agcaatcatc tcgagcagaa tctatacaaa atctgaattt
     3721 tattgaaact gttcatgact tatgtcacca agatcaatta gaattcccgg aggtaagaaa
     3781 ttttggtaaa tttataaggc gcgagcgggt ttttcaaaat accctaaaac gtgcgtttat
     3841 tgttatggtt gactcagaag agctggcagt caaaataaat cttcgatatg gtgttgatcg
     3901 agatcgtatt gttaatatgc ctttttcgcc atcccctttt ctcaatgaaa ctaaagcctc
     3961 agatagtgca gatatattga aattgtataa acttgattac ggatactttt tttatccagc
     4021 acaattttgg gctcacaaaa accattatag aattatcgaa gctgttaaat tacttcatca
     4081 agaaggcaaa caatgtcgag tggtttttgt tggaggcgac aaaggaaatt atgatttttt
     4141 agatcgtctt ataagagggt atggcttagg caagcacgtt caatctttag ggtttttacc
     4201 tgcagaacat atgagggcat tatatgaaaa ttgtcaggca gtaataatgc ctacctattt
     4261 cggttcaaca aatttaccac ctctagaggc gtggcttatg ggaaaaccgt tgatctattc
     4321 caaactttgt tctgaacagg ctggaaatgc agcattattg atcgaccctg atgacacgaa
     4381 ttctgttgct gatgcaatga aatccatgtt agacgaatct atatcaaaaa aatataaaaa
     4441 aaacggttat gagcgcctca agcaaatatc aaccatgaga aaaatatctg agagcaattt
     4501 gtccgcaaag ctacttcagt ttgaacaaaa aatgaaatgc tgggaatgat atgtttccct
     4561 aaatagtaat ggatgttctt tttcaatttt aatatttggg gtaatattga aatcatttat
     4621 taaacgaatg cttcataaga caggttttga gttacgacgt ttttctgttg aacattcgga
     4681 aaacgctcga tttatatcaa tgcttagagc taacaatgta aatttaatat ttgatgttgg
     4741 agcaaataca ggacagtttg gagttttgat gcgcgaaatt ggttttgatg gaaaaattct
     4801 ttcttttgag cctctttctg aggctaggga aagtttgcta aaaatcagta gaaatgactc
     4861 catgtggcaa gttgcacttc aatccgcaat aggtgaagaa aatggag
//
LOCUS       DASCPJ010000059         3688 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000939335, whole genome shotgun sequence.
ACCESSION   DASCPJ010000059 DASCPJ010000000
VERSION     DASCPJ010000059.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000939335"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="QGF01_02665"
     CDS             complement(<1..2001)
                     /locus_tag="QGF01_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJN19833.1"
                     /translation="MEKLDQKISEKTQTETFLQEKFRLLGFKQFTNIQKKAAPIVSQK
                     KNSLIIAPTGSGKTECTVIPIFSLIKKTKQQGKIKALYVTPLRALNRDVFRRIIKYAD
                     YDDLTIQVRHGDTSQSLRKKISDSPPDILITTPETLVILLTQKKFLVALAELEWVIID
                     EIHELLASERGSQLSLSLERLQLNASQKIIRIGLSATIGNIDDAAQFLVGTNKPCAII
                     QDESVRNYDVEVKFVNGSISEVADSVINYVEKSNFDSPILLFTNTRGESEFLASILRD
                     KTMMNIELHHGSLSKQVREETESILRSGRKGIVVCTSSLELGLDIGSVDLVIHYGSPR
                     QVSKLVQRIGRSRHTRNSSAKGLIITNNGDDEFETTAILDRVKNNSIENQNIHDESFD
                     VLAHHLVGLSMQIGEVPIDFAHKIITQAYPFRNLTLDDLCDVLEILHSISRPILFFDK
                     PKMKFWKKGNSFRYYFENLSTIPDILKFKVFDSVGKKLIGSLDQRFVGDFGEQGNIFV
                     LRGLQWRILNVDEKSLQVNVEPIKSGGKKVPYWEGENIPVDFATAQKVGLLRKKVRDG
                     SLSMINQTIPNLDFDVTPDEKTIVVESLRTEGTVVIHACFGTRINSTLATILSSLLSS
                     VLGYTVESRSDAYRIVLTSNSRIHKKIFVEALIEKFDINDVVS"
     gene            complement(2035..2685)
                     /locus_tag="QGF01_02670"
     CDS             complement(2035..2685)
                     /locus_tag="QGF01_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020014.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HJN19834.1"
                     /translation="MISTGLQKLDACLGGGLKEGLITDIFGKNATGKSQLAFQICINA
                     LKSGKEVLFEDTTGGFRPERLVEMIGSQNMDLKILDKIKIARITDSIQQIQYLSKIPI
                     DNFSLIIIDNVSDLFSFEYSKKNNFLEKHLSFMKYMYDLSRISITTRIPIVVTNIVRN
                     TDDNEIENLEKSISMYSHVKIKLSKINHEYTCQIISPFSSQKISYVISSGGLTNPS"
     gene            2789..3031
                     /locus_tag="QGF01_02675"
     CDS             2789..3031
                     /locus_tag="QGF01_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0000398 - mRNA splicing, via spliceosome
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U6 snRNA-associated Sm-like protein LSm6"
                     /protein_id="HJN19835.1"
                     /translation="MSQTTSKRPLTVLQKNTKKDVIVRLKSEVEYKGKMENVDSYMNL
                     IMTDAEEVNQGKTVGKFGRVIVRGNNVLFIKLENEF"
     gene            3045..>3688
                     /locus_tag="QGF01_02680"
     CDS             3045..>3688
                     /locus_tag="QGF01_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HJN19836.1"
                     /translation="MAKNYIFTSESVTEGHPDKVCDHISDAILDEFLRQDPDSRVAVE
                     TLVTTDFVTVAGEVTSKGKFDIEKVVRDTIAEIGYDDAALKFDAKTCKVLVKIDPQSP
                     NISQGVTVSQTQAEQGAGDQGLMFGYATNETDELMPLPILLAHKLTKKLADVRKSKEL
                     AWVRPDGKSQVSVEYVDNKPQRVQTVVISTQHDPEISNEEIRKEIIDKVIKPVCG"
BASE COUNT         1227 a          589 c          581 g         1291 t
ORIGIN      
        1 agaaacaaca tcatttatat caaatttttc aatcagagcc tcaacaaata tttttttatg
       61 tattcgtgaa ttagatgtta aaacaattct atatgcatct gaacgtgatt ctacagtata
      121 tcctagtacg gatgatagaa gtgatgaaag tatagttgct aaagttgaat ttatccttgt
      181 accaaaacat gcatgaatta caaccgtacc ttctgttctt aatgattcaa ctacaattgt
      241 tttctcatca ggtgtaacgt caaaatcaag attaggaatt gtttggttaa tcattgataa
      301 tgaaccatct cttacttttt ttctaagtaa accaactttt tgtgctgtgg caaaatcaac
      361 tgggatgttt tcgccctccc aatatggaac tttttttcct ccacttttaa taggttcaac
      421 attaacttgc aatgatttct catcaacatt caaaattctc cattgcaatc cacgaagaac
      481 aaaaatattt ccttgttcac caaaatctcc aacaaatctt tgatctaatg atccaatgag
      541 ttttttccct actgaatcaa aaactttgaa ttttaaaata tctggaattg ttgaaagatt
      601 ttcaaaataa tatctaaaag aatttccttt tttccaaaat ttcatttttg gtttatcaaa
      661 aaataaaatt ggtctactaa ttgagtgtaa aatttctaaa acatcacata aatcgtctaa
      721 tgttaaattt ctaaatggat atgcttgtgt gattatctta tgtgcaaaat ctattggtac
      781 ttcacctatt tgcattgata atccaactaa atgatgagct aggacatcaa aagattcatc
      841 atgaatattt tgattttcta ttgaattatt ttttacccta tcaaggattg ctgttgtttc
      901 gaactcatca tcgccattat ttgtaataat caatcctttg gcagaagaat ttcttgtatg
      961 tctacttctt cctattcttt gtactagttt tgatacttga cgtggtgaac cataatgaat
     1021 gacaagatct acagatccta tgtccaaacc caattcaaga gatgatgtgc aaactactat
     1081 tccctttctt ccacttcgta gtatgctttc agtttcttct ctgacttgct ttgataaaga
     1141 cccatgatgt agttcaatat tcatcattgt cttgtccctc aatattgatg ccaaaaattc
     1201 cgattcgcct ctggtattag taaacaagag aattggggag tcaaagtttg atttttctac
     1261 ataattaatg actgaatcag cgacctcgga aatagaacca ttgacaaatt ttacctcaac
     1321 atcataattt ctaactgatt catcttgaat tattgcacat ggtttgtttg ttcctacaag
     1381 aaattgcgca gcgtcatcaa tatttccaat cgtagcggat aacccaatcc gtataatttt
     1441 ttggcttgcg ttaagttgaa gacgttctag acttaatgaa agttgagaac ctcgttcact
     1501 tgctagaagc tcatgtatct catcgatgat aacccattct aattcagcaa gtgcaaccag
     1561 aaatttcttt tgggtaagta agatgacaag cgtttctggt gtagtaatta atatgtcagg
     1621 tggtgaatct gaaatttttt ttcgaagtga ttgtgatgta tctccatgcc ttacctgaat
     1681 tgtaagatca tcataatctg cgtacttgat tattctacga aagacatctc ggttgagtgc
     1741 tcttaatggt gtaacataaa gtgccttaat ctttccttgt tgcttcgttt tttttattaa
     1801 tgaaaaaata ggaattactg tacattcagt tttcccagaa ccagttggtg caattatcaa
     1861 tgaattcttt ttttgagaaa caatgggtgc tgctttcttc tgaatatttg taaattgttt
     1921 aaatcctaat aagcgaaatt tttcctgtag aaaagtttca gtttgagttt tctccgatat
     1981 cttttgatct aatttttcca taagacatct agtagttttt ctgtgttaaa taaattaaga
     2041 tggattagtt agaccaccac tagatataac ataagaaatt ttttggctgc taaacggtga
     2101 aattatttga cacgtatact catgattgat ttttgaaagt tttattttga catgagaata
     2161 catgcttatt gatttttcta aattttctat ttcattatca tcagtatttc tgacaatatt
     2221 cgtaaccact ataggaattc tagttgttat ggaaattcta gataaatcat acatgtattt
     2281 cataaaagaa agatgttttt caagaaaatt attttttttt gaatattcaa atgaaaacag
     2341 atcacttaca ttatctatta taattaatga aaaattatca attggtattt ttgacaaata
     2401 ctgaatttgt tgtatgctgt cagtgattct tgcaatctta attttatcaa gtatttttaa
     2461 atccatgttc tgcgagccta tcatttcaac taatctttct ggtctgaacc cacctgttgt
     2521 atcttcgaat agcacttctt ttccactttt taatgcatta atacaaattt gaaaagcaag
     2581 ttgtgattta cctgtagcat ttttaccaaa aatgtcagtt atcagacctt cttttaaccc
     2641 accaccaaga catgcatcta atttttgtaa tcccgttgaa atcatactct tagtacttgc
     2701 agtaaaaatt aaacatattt cataattacc aaaataggca aggcttttat tttggtaaac
     2761 aaggaaacca cacaagcgag aattatttat gtcacagaca acatcaaaaa gaccattaac
     2821 tgtattacaa aaaaatacta aaaaagatgt tatagtcaga ctaaaatctg aagtggaata
     2881 taaaggaaaa atggaaaatg ttgattctta tatgaatttg ataatgactg atgctgaaga
     2941 agtaaatcaa ggaaaaactg ttgggaagtt tggaagggtt attgtacgag gaaacaacgt
     3001 gttatttatc aagttagaaa atgaatttta atctgtttta acaaatggca aaaaactata
     3061 tttttacatc agaatcagta actgaaggtc atccagataa ggtatgcgac catatttctg
     3121 atgcaatatt agacgaattt cttcgtcagg atccagattc tagggtagcg gtagagacgc
     3181 ttgtaacaac agattttgtc acagttgctg gtgaggttac atcaaaagga aaatttgaca
     3241 ttgaaaaagt tgtaagagat acaattgcgg aaattggtta tgatgatgct gcattgaaat
     3301 ttgatgcaaa aacttgcaaa gttcttgtaa agatagaccc acaaagtcca aatatttctc
     3361 aaggagtaac agtatcgcaa acccaagcgg aacaaggagc gggagatcag ggtttgatgt
     3421 ttggatatgc tacaaacgaa actgatgagc ttatgccatt accaatatta ctggctcaca
     3481 agcttaccaa aaaacttgct gatgttagaa aatcaaaaga attagcatgg gtaagaccag
     3541 acggtaagtc tcaagtttct gttgaatatg tagataataa gcctcaaaga gttcaaactg
     3601 ttgtaatttc aactcaacat gatcctgaaa tatcaaatga ggaaattaga aaggagatta
     3661 ttgataaagt gataaaacct gtttgtgg
//
LOCUS       DASCPJ010000060        20348 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000975279, whole genome shotgun sequence.
ACCESSION   DASCPJ010000060 DASCPJ010000000
VERSION     DASCPJ010000060.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 20348)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20348)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20348
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000975279"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="QGF01_02685"
     CDS             <1..277
                     /locus_tag="QGF01_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJN19837.1"
                     /translation="TESGASFMTGFNQFYYSFSPAIADYERENPVFKEVVKLTITPLL
                     TSLTILNYVDIDTEEEMLGYGIGVILLNIGMYFVAPAAILFKIKNRK"
     gene            315..638
                     /locus_tag="QGF01_02690"
     CDS             315..638
                     /locus_tag="QGF01_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19838.1"
                     /translation="MNVEFEEFSTVEEIFTYMASIAPPMKNYLPINSYKGYICSIIPL
                     SQSGGETFLMVFTKGSLDNGILEFDINTNTYKKVESMERADKNYFIVMSPKRNTIADA
                     AIEKL"
     gene            complement(643..2169)
                     /locus_tag="QGF01_02695"
     CDS             complement(643..2169)
                     /locus_tag="QGF01_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN19839.1"
                     /translation="MTNYRGNFLYGFIACGPYEVLPEWVFDKVFCPAVETDPITGEAK
                     VAQVGLRRVESALLQGYKRDEIMTVNPDYIEKSIGPDTKIVGINVMDPLGMAPVTTTM
                     SPEKLSYVAMKFKKLCANIIQLKKKYDFKVVVGGNGAWELAKSDRMKVHGIDTVVVGE
                     ADELALDLFHELETGNAPELMHCYVKNIQNIPEITAPTVNSLIEAMRGCGRGCDFCDV
                     NKRSKKDLPLERLQREAKINLDYGFDSVWLHSDEMLLYGCDNRDFQPNYDAITTLWRG
                     LKDLGANFVGTTHMTFSAVAADPKLIKDISEINDMHKSGRWISTNLGIETVAPDMVKK
                     HLGIKAKPFSADEWGWVVREGAQILNKNHWFPAATIIIGWPDETPDQVQYTIDMMRDF
                     RAFNFRGLVAPLLYQDFSEKNSMHFGNLNEAQFTLFWKCWENNLRVINDIIPIILRNK
                     TYGPPMKIFMYGLIKAGTWAIMRYLRGLCKDLFNGRMPEEIIDKYARSRSVTASTYIK
                     "
     gene            2305..3027
                     /locus_tag="QGF01_02700"
     CDS             2305..3027
                     /locus_tag="QGF01_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HJN19840.1"
                     /translation="MKEISEPRHTESHIAESSTIRDFVFGFGDGINTSLGIAAGVGGA
                     NVASDIIVLAALVGMFTGAKAMAVQNYLAVKSQRQLLESEIEREKWEIENKPDIERQE
                     IEDIYKAKGFAGKDLEMIVNKITSDKKVWLDTMLSEELKLNVDIVGSPLKSALIMFVS
                     FLAGGMLPIVPFFFGSGYSALAIAIGISVTASFIVGAIKSRMANTGIVKGGLEMAGLG
                     TGVALIGFGIGTELANLGIINV"
     gene            complement(3017..3796)
                     /gene="larB"
                     /locus_tag="QGF01_02705"
     CDS             complement(3017..3796)
                     /gene="larB"
                     /locus_tag="QGF01_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HJN19841.1"
                     /translation="MNIEQIFQSLQDGKISPSKAKKLLSLYSIEKIGNIAQIDTGRRN
                     RKGIPEIILAERKELLDLKKIIKKTLTKNNEVLISRIKQKDYTKILEFAKKNKYKIKN
                     GKNTTALLIYKNKIKKLGGKVGIIAAGTSDISVGEEARLVCESMNCDCVCSYDVGIAG
                     LHRIFPVLKQFIESEVDVIIVVAGMEGALASIVSSIADVPVIGVPTSIGYGYGEKGVA
                     ALASMLQSCSLGLTVVNIDNGVGAGAAAANIANKIKNKSTR"
     gene            complement(3832..4746)
                     /locus_tag="QGF01_02710"
     CDS             complement(3832..4746)
                     /locus_tag="QGF01_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HJN19842.1"
                     /translation="MITIIGAGKVGGSAALCSAIKQLDDQILLLDIAEGLPQGEAMDL
                     NHLLSEQGIDVEIKGSNNYEDMKGSDIVVVVAGAGRKPGMTRMDLLKINAGVVKNVVG
                     NIKKYADNSLIIPVTNPLDPMAYITFKTSGFEKNRVFGMGGMLDLSRFKQFIHEASDC
                     PRKEIDAIVIGEHGENMLPLTRLAQISGKPLPTVLSQEKLDEIFTLTKNVAAEVIKLK
                     GATVHAPGNAISSMIESVVKDKKQVMPVSTYLDGEYGYSDVTIGVPAVIGKNGVEKIN
                     ELDLNQDEKEWFDKGVNSVKSALAGIDI"
     gene            4833..6053
                     /gene="larC"
                     /locus_tag="QGF01_02715"
     CDS             4833..6053
                     /gene="larC"
                     /locus_tag="QGF01_02715"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HJN19843.1"
                     /translation="MVLVIDSQIAGISGDMLLCSLVNMGANRSKIIDGIRNAESLCKD
                     VKVKKVEFVEVKKNSLQATALLLEIDDDVHERKGIEIKEIIEKSAEKLTISESAKTFA
                     IKSIETLIRAESKIHGEPENSVHFHEAASFDTVIDILGTAIALDDLGCFDDDIVGTPV
                     AIGGGTMIFSHGISSNPAYAILEIFRESGIITVGGNVKDEMTTPTGASMLVNLIKECS
                     EFYPPMKIQSIGYGAGQKDFEGFSNVLKIVRGVVSTKLQLDTVKILETNVDDVSGEVL
                     GNMIEKIMAYGAKDVTISSAITKKGRPTNLVSVICDSDTMNSIMDLLVTETGTLGVRV
                     RTSERYIVPRSVKTISVNINGQSFDIRFKIRDLNNDSHFKIESDDIKEISGVLSISFK
                     ETEELLNQEIRKKL"
     gene            6050..6853
                     /gene="larE"
                     /locus_tag="QGF01_02720"
     CDS             6050..6853
                     /gene="larE"
                     /locus_tag="QGF01_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HJN19844.1"
                     /translation="MKTHLTKLLNWFDDKNNVLVALSGGVDSALVAYSAYAKLGKSAI
                     AVTADYKTLAQEELEYAKKISSEIGIKHLVIEYNELDNESFVKNDNNRCFYCRNELSE
                     HLLEIAKESGSDIIVDGTNTDDLGDYRPGVLALHENGIRSPLAETKFSKKMVREVAKN
                     VGLSVYDKPSNSCLASRIPWGQSVTAERLARIEVGEKIVKQLTGAKQVRVRDMNGIAR
                     IEVGVDELSLLENNLVLQKIKDQLKLISFDDISIDPEGYRPGKINVITN"
     gene            6850..8016
                     /locus_tag="QGF01_02725"
     CDS             6850..8016
                     /locus_tag="QGF01_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HJN19845.1"
                     /translation="MIYLDNAASTEIHPEVVKEMLPYFDKYYGNPSSIHQFGRKAKNA
                     IEKARKQVAALIGAEPNEIIFTSGGTESNNTIFYGIISQGEKFELSHIITSSIEHDAV
                     LEPIRQFEKNGCQITYLPVDKHGMINLDDITKSISKQTVMISIMFANNEVGTIQPIKE
                     ISKICNKYQIPLHTDAVQAVGKIPINVKELGVDALSISSHKINGPKGVGALFLKKGST
                     TTPYIVGGGQENGMRSGTENVASIVGFGKACEIAKDKLSDNISHFQTLHSSMLSRIVK
                     EIPHVKLNGHPQNRIFNNIHLTFLGINGEDLIIKLDEHGIAASTGSACSVHTQKASHV
                     LKAMGFNHEQITGSLRISFGYTNTLDEVEQTVEVLKKVVSELRSVSPYKTKYNF"
     gene            complement(8021..8821)
                     /locus_tag="QGF01_02730"
     CDS             complement(8021..8821)
                     /locus_tag="QGF01_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HJN19846.1"
                     /translation="MIITISIIPVFAQDSESITVSLNKETYTQGDTIVVFGEVKKMIG
                     GLPSITIQIYHSGNLVDVAQPQLANDGTYAESIFASGKLWKTNGTYLVRVFYTEEIVT
                     ETNFEFYNTQQTTQPSSTLSVNIPNASEVFDVGYAITTGEVTDIQIDKDNYSLVTDFN
                     IDAAGDLVLSLPRDSFDAKTTEGSDDVFIVLITNISGDTFEVEYEETQTNAEYRTIKI
                     PFVEGDYKIQIIGTYVIPEFGTIAIMILLISIISVIILSKNKFSLVHN"
     gene            complement(8928..9743)
                     /locus_tag="QGF01_02735"
     CDS             complement(8928..9743)
                     /locus_tag="QGF01_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HJN19847.1"
                     /translation="MIRKGLFILIGIMAIASASLALQPIYADDGITIETDNDVYNHSS
                     TIILTGHITVVDQNEVPVVIMVISPNGNIANIAQVSVDSDGVFSTTFNTAGAMMRFDG
                     TYQIQASYGFVETTISIELTNAIESAMTSDTMETAETTLEDAIKDGQINWTIDGGVIT
                     SFFVNGDENSIVLYIEATDDGILDITLHEDIIKPFDDGTFFVTVNSEESQDFVQNVNE
                     LSIPISADTEKITIFGGWAIPEFGTIAIMILVVAIVSIIALSAKTKLSLVPRY"
     gene            complement(9828..10628)
                     /locus_tag="QGF01_02740"
     CDS             complement(9828..10628)
                     /locus_tag="QGF01_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HJN19848.1"
                     /translation="MTSLSIVFLAVAMISVSALSQNAYAASGLSLSADGSGDTIAISG
                     HSDRSDLDVTIVLSNSMGIHGVAQSSVSGGSYSASFNVSMLDGSYTITANQGDSSKYS
                     VSVDVDVSDGTSDSSVSATTITAADVTIEEVGSASIRGLTITADAIEGATTVDVSGST
                     DRTGDIVLTVTAPNGNIVSVAQVSPSGGSYMTTISVGGALWSQDGMYTISAQQGEAAL
                     YKTSTQVDIKDGHVVPEFGTIAIMILVVAIVSIIALSAKTKLSLVPRY"
     gene            10839..11090
                     /locus_tag="QGF01_02745"
     CDS             10839..11090
                     /locus_tag="QGF01_02745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HJN19849.1"
                     /translation="MVSKKNIVLVPDHILVPKHEIMAKKDAEEVLKTYNCRTTDLPLI
                     FVNDPAIVGLGVKPGDMIKITRKSATAGESFYYRYVVEV"
     gene            11087..14431
                     /locus_tag="QGF01_02750"
     CDS             11087..14431
                     /locus_tag="QGF01_02750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HJN19850.1"
                     /translation="MTFSSDKRWPVIQNILITQGIAKQHLNSFDEFLENGLQEIINEV
                     QQIEIENAEYPYTIKLGKIKFQEPRMMELDGSITHTTPAESRLRNVSYVAPLQMEASV
                     VEDGKTLESKPIHIGDIPVMVKSNACILRSFTDQKLIDHGEDPEDPGGYFIINGSERV
                     IVGLEDLSYNKIIVDTDKVGGKYIHKAKVYSSIVGYRAKLELILKDDGLIVAKIPGSP
                     VDIPVITLIRALGLETNDEIINAVSLVDQIQAELESSFEKTEVNTSKDAIVYISKRIA
                     PGMLEEFQIKRAETLLDWGLLPHLGKHPESRREKAQFLGEAACKLLELKLGWIEIDDK
                     DHYGNKVIKFAGQMLADLFRTAFRNLVRDMKYQLERSGQKRGINAVAAAVRPGIISDK
                     LNNAIATGNWGRGRVGITQLLDRTNYLSTISHLRRVQSPLSRTQPNFEARDLHSTHFG
                     RICPSETPEGSNCGLVKNLALSAIISTSVDSSDIRQKLDDEGVADFLDVNDSVKKTGA
                     RVFLDGKFIGYFPDGEKLVLTLRELRRNNKINSHVGISLHTPEEEGATKRLYVNCNAG
                     RVLRPVILIKDGKSLLTNDLIEKTSKKLVSWLDLIRMGVIELIDANEEENCDITFDEK
                     NTKKFTHLEIFSSAILGAGASIIPYPEHNQSPRNTYESAMAKQSLGFSTPMMNTSTYV
                     RQHFMLYPQTPIVSTKAMNLLGLEKRPAGQNCVVAVLPFDGYNIEDAIVLSQSSVDRG
                     LGRTFFYRIYESEAKQYPGGMRDSFQIPNADENIRGYKGEKSYRLLEEDGIVATESTV
                     KGGDILIGKTSPPRFMEDYREFESQGPYRRDTSVGVRPTENGVVDTIVMTQSNEGGKM
                     FKVRVRDMRIPEIGDKFASRHGQKGVLGILAKQEDLPYTADGISPDVLINPHAFPSRM
                     TVGMMMESITGKAAALRGAKFDGSAFVGEKIDQVRDVMKDAGFKYSGKEIMYDGRTGK
                     PFPVEVFIGIVYYQKLHHMVADKIHARARGQVQMLTKQPTEGRARGGGLRFGEMERDC
                     IIAYGASMILKDRLLDESDKDDIFVCERCGLVAYHDIKQRRYICRVCGDKGKVSSVSV
                     AYAFKLLLQEMQSLNIAPRLMIKEKI"
     gene            14431..18222
                     /locus_tag="QGF01_02755"
     CDS             14431..18222
                     /locus_tag="QGF01_02755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HJN19851.1"
                     /translation="MSIETIKSIQGIRFSVWSPSEIRKYSVAEITAPETYDEDGMSVQ
                     GGLMDGRLGTLEPGQKCLSCGNTSARCPGHFGHIEFAEPVLHIAFIDSIHKLLLCTCR
                     SCSRLKIPSDDLERLANMKKQKASYTIISQKRVPEQILEKAKKQKECPHCGKVQYDLI
                     FTKPTIFIEKTELGENRLLPITIRERFSQITNDDLDLLGYDHTTARPEWFVLQVLPVP
                     PVTVRPSIILETGIRSEDDLTHKMVDIIRVNQRLKESKEAGTPPLIVQDLVDLLQYHT
                     TTYFDNEVSGIPQAHHRSGRPLKTLTQRLKGKEGRFRGSLSGKRVDFSSRTVISPDPN
                     LDLSEVGVPVPIAMKLTIPEIVTEWNIEKLKKLIINGPSTFPGVNYVVRPDGVKIRLD
                     FVEDRSIIADSIETGYLIERHLLDGDIVLFNRQPSLHQMSIMAHHVRVLPGKTFRLHP
                     SVCPPYNADFDGDEMNLHVPQSEEARAEAILLMRVQEQLISPRFGGPIIGGLRDFITG
                     AYLLTKDDTILTSQEFSNYAMLGGYKGKLPEPSSKNKTGASYTGKQLFSIFLPSDFNY
                     IMTSKWSKGTKGTNQDVVIKNGELISGVIDKSSIGAEEPESVLHRIAKDYGNERAKNF
                     LNSVLIIIKQFITDYGFSYGYSDLELSDKDREAILDDIQATYDKVGDIITEKNKGTLK
                     GLRGMTVSETAEAKITFELAKARDRAGITANSNLEDNNAGKIMATTGARGSALNVGQM
                     AGALGQQSRRGKRLHTGYANRTLPHFKTHDENPDSHGFVKSNFRDGLSTLEFFFHAMG
                     GREGLVDTAVRTQQSGYMQRRLINALEHIKLEYDGTVRDPHRHIIQFLYGEDGIDVAK
                     SDHGEAFNINRLIESQRIIDSGKAATKEEITSLTKKYSKTFNPRLTSIVSDGLLDSKL
                     SKVGIESVCKKGLELYDNCKAEPGQAVGIVTAQSIGEPGTQMTLRTFHTAGIAEKNVT
                     LGLPRIIELVDARKKPATPSMDIYLDKDSKVSQEKAVEVARDIKETKISDLLIRTVSD
                     HATEIKLEFDMKKLETRHVTLEQVTSLLESNKKFKLEFSKNTIILKLIEESDSVTVNT
                     IKNKVLKSTVRGVSDIERVTLKHDNGEWIIQTTGSNVAKILHVKGIDKQNIRTNNVYE
                     IAETLGIEAARNSLINELKFTLENQGLEVDTRYLMLVSDLMCHKGYLQQIGRHGIAGS
                     KDSVLARAAFEITVPTIAGAAKLGQIEELKGITENVIVGSQIPIGSGTVDIYMESATK
                     T"
     gene            18313..18624
                     /locus_tag="QGF01_02760"
     CDS             18313..18624
                     /locus_tag="QGF01_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HJN19852.1"
                     /translation="MAKLLEKTLKDAIKENNHVIGTKQVMSSIKSSKLVIISQSIPSP
                     TIQQIEDNTKKENIPLLHFEGTSIMLGKLCGLQFRVSTISLKSLSNTNIQAILKESET
                     K"
     gene            18694..19158
                     /locus_tag="QGF01_02765"
     CDS             18694..19158
                     /locus_tag="QGF01_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HJN19853.1"
                     /translation="MTNSIKLTTEQMRFISLFQNVTKSSARDCLDDEKQDRLIFVVNE
                     GKMGLAIGKGGSNIKSLQNILKRNVELIEYNEEPIKFLKNILNAKLISEIKLNTKPDG
                     SLHAVVFVDPGKKGIVVGREGRNAEKARLLAKRYFNISTVMINSQEMTQLEV"
     gene            19162..19599
                     /locus_tag="QGF01_02770"
     CDS             19162..19599
                     /locus_tag="QGF01_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HJN19854.1"
                     /translation="MAKSPLGLFAGRVLKEKKKRQRWSISRYKRRELGLDKKSNPLGG
                     APQARAIVLEKVGVEAKQPNSAVRKCVRVQLIKNGKSVTAFLPRDGAMNFIDEHDEVH
                     LEGMGATQGGAMGDIPGVRYKVFKVNGTSLKQLVTGKKEKPRR"
     gene            19604..20200
                     /locus_tag="QGF01_02775"
     CDS             19604..20200
                     /locus_tag="QGF01_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HJN19855.1"
                     /translation="MTEQKMLLFRRWDLSTIEIKDSGLKNAICLKPSILPIDFGRSAL
                     RRFNKADVNIVERLVNKLMHFGKKYSKNTGRMAGKKDHSFNVVKTAFEIIHLKTEKNP
                     IEVLVRAVENSAPNEDTTRIVYGGTVYHVSVDVAPIRRVDLALRFIADGVKEATFSNP
                     KPIEEYLAEHLILAANNDPTSPSVKKRNELERIAQASR"
     gene            complement(20210..20281)
                     /locus_tag="QGF01_02780"
     tRNA            complement(20210..20281)
                     /locus_tag="QGF01_02780"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20246..20248),aa:Gln,seq:ctg)
BASE COUNT         7048 a         3346 c         3706 g         6248 t
ORIGIN      
        1 aacagaatct ggtgctagtt tcatgactgg attcaaccag ttctactatt cattcagtcc
       61 agcaatagct gactatgaac gagaaaaccc agtattcaag gaggttgtga aattgacaat
      121 aactccactt ctaacatcac taacaattct aaactatgtt gatattgata ctgaagagga
      181 aatgttagga tatggaattg gagtgatact tcttaacatc ggaatgtatt tcgttgcacc
      241 tgcagcaatc ctgtttaaaa tcaaaaatag gaaataatct ttaggttatt atactttaaa
      301 atatttcaga cagtatgaac gtagaatttg aagaatttag taccgtagaa gaaatattca
      361 catatatggc ttctattgct ccaccaatga aaaattattt acctatcaat tcttacaaag
      421 gctatatttg ttctataata ccattgagcc aatcaggtgg tgaaacattt cttatggttt
      481 ttacaaaggg ttcgttagat aatggaattt tagaatttga catcaatact aatacttaca
      541 aaaaagttga atcaatggaa agagcagaca aaaactattt catagttatg tcaccaaaaa
      601 gaaatactat agctgatgca gcaatagaaa aattataatc tattatttta tgtaagtaga
      661 agccgtaacg gatctacttc ttgcatattt gtcgatgatc tcttcgggca ttctaccgtt
      721 gaacaagtcc ttacataatc cacgcagata tctcattatt gcccacgttc cagctttaat
      781 gagaccatac atgaaaatct tcattggggg tccatatgtt ttgtttctaa gaataattgg
      841 tataatatca ttaataacac gaaggttgtt ttcccaacat ttccaaaata atgtgaattg
      901 tgcctcattg agattaccaa aatgcattga atttttttcg ctgaaatctt ggtatagtag
      961 tggagctact aatcctctaa aattaaatgc tctaaaatca cgcatcatat caattgtgta
     1021 ttgtacttga tcaggagttt catctggcca accaataatt atagtggcag ctgggaacca
     1081 atggtttttg ttaagtattt gtgctccttc tcttactacc caaccccatt catcagcaga
     1141 gaatggtttt gcttttattc ccaagtgttt ttttaccata tcaggagcaa ctgtttcaat
     1201 ccctagattt gttgatatcc atcttccact tttatgcatg tcgttaattt ctgaaatatc
     1261 cttgatgagc tttggatcag cagcaacagc tgaaaatgtc atgtgagttg taccaacaaa
     1321 attagcacca agatctttaa gccccctcca taacgttgta attgcatcat aatttggttg
     1381 gaaatctcta ttatcacaac cgtatagtaa catctcatca gaatgtaacc aaactgaatc
     1441 aaatccataa tcaagattaa tcttagcctc acgttggagt ctttcaagcg gtaagtcctt
     1501 tttagatctt ttatttacat cacaaaagtc acagcctcta ccacaacctc tcatagcttc
     1561 aattagtgaa ttcacagtag gggcagtaat ttctggtata ttttgtatat ttttaacata
     1621 acaatgcatt aattctggcg catttccagt ttctaattcg tgaaataaat ctaaagcaag
     1681 ctcatctgct tcaccaacaa ctacagtatc aataccatga actttcattc tatcagattt
     1741 tgcgagttcc catgctccat tacccccgac aacaaccttg aaatcatatt ttttctttaa
     1801 ttggataata ttagcacata actttttaaa tttcatcgca acgtaagaaa gtttttctgg
     1861 tgacatagta gttgtaactg gtgccattcc aaggggatcc attacattga ttcccactat
     1921 cttagtatca ggaccaattg atttttcaat ataatcagga ttcacagtca taatctcatc
     1981 tctcttgtaa ccttgtagta atgcactttc cactcttctt aatccaactt gggcaacttt
     2041 tgcttcacca gtaataggat cagtttcaac tgcaggacaa aagactttgt caaaaaccca
     2101 ttctggaaga acttcataag gaccacatgc tatgaaacca taaaggaaat tacctctata
     2161 attggtcatc aaactacgat ccgctgtaag tactattctt ttaccagcca atgtaagctt
     2221 gaccttgtcg atagtataaa atttttgcga caagacatct aatatttacc aaaaattcga
     2281 ttactaatag ggtcttgaaa taaaatgaag gaaatcagtg agcctagaca caccgaatca
     2341 cacattgctg aatccagcac cattagagat tttgtttttg gatttggaga tgggataaac
     2401 acttcccttg gtattgctgc tggtgttgga ggtgcgaacg ttgcctctga tattatagtt
     2461 ttggcggcat tagtgggaat gtttactggt gcaaaggcta tggctgtaca aaattatctt
     2521 gctgtgaaat ctcaaagaca attgttagaa tcagaaatag agagggaaaa atgggagata
     2581 gaaaataaac ctgacattga acgtcaagag attgaggata tttacaaagc aaaaggattt
     2641 gcaggaaaag accttgaaat gattgtaaac aaaattactt ctgacaaaaa ggtctggcta
     2701 gatacaatgc ttagtgaaga actaaagcta aacgtcgaca tcgttggaag tccactaaaa
     2761 agcgcattga taatgtttgt ttcttttttg gctggaggta tgcttcctat agttccgttc
     2821 ttttttggta gcggttatag tgcgcttgca atagcaatag gaattagtgt aactgcttca
     2881 ttcattgttg gtgccataaa atcacgaatg gctaacactg gtatagtaaa gggtggttta
     2941 gagatggctg gtcttggaac cggggttgca ttaattggat ttggtattgg cactgaactt
     3001 gcaaacttgg gaataattaa cgtgtagact tattttttat tttatttgca atatttgcag
     3061 cagcagcacc agcaccaaca ccattatcta tattgactac agtaagacca agtgaacaac
     3121 tttgtagcat ggatgcaagc gcagcaactc ctttttcacc atatccatat ccaatcgagg
     3181 taggtactcc aattacaggt acatcagcta tagatgatac aatagatgct aatgcgcctt
     3241 ccatcccagc tactactatt ataacgtcaa cttctgattc gataaattgt ttaagaactg
     3301 gaaaaattct atgtaatcct gcaatcccaa catcataact acaaacacaa tcacaattca
     3361 tggattcaca aacaagtctg gcttcttccc ctacactaat atctgatgta ccagcagcaa
     3421 ttattccaac tttaccacct aattttttaa ttttattttt ataaattaac agtgcagtcg
     3481 tatttttccc atttttgatt ttatatttat ttttctttgc aaattctaaa attttagtat
     3541 aatctttctg tttaatcctt gaaataagta cttcattatt tttggttaaa gtttttttga
     3601 taatcttttt caaatctagg agttcttttc tctcagcaag aataatctcc ggtattccct
     3661 ttctatttct tcttccagta tcaatctgag caatattacc tatcttttca atcgagtata
     3721 atgataataa ttttttagct tttgatggtg aaatcttccc atcttgtaat gactgaaaaa
     3781 tttgttctat attcaattat ttaccttaaa tttgattagt taaaaaaatg atcagatatc
     3841 aatccctgct agggcacttt taacactgtt tactccctta tcgaaccatt ccttttcatc
     3901 ttggtttaga tcaagttcat tgattttttc cacaccattc tttcctatta ctgcaggaac
     3961 accaattgta acatccgaat aaccatattc tccatccaag tatgtggaaa ctggcataac
     4021 ttgtttttta tctttcacaa cgctttcaat catagaggag attgcatttc caggtgcatg
     4081 aaccgtagct ccttttagct ttatcacttc agctgcaaca ttttttgtca aagtaaaaat
     4141 ttcatctaat ttttcttgag aaagtactgt gggtaaaggt ttgccagata tttgagctaa
     4201 tctagttaat ggtaacatat tttccccatg ttctcctatt actatcgcat caatttcctt
     4261 ccgtggacaa tcacttgctt catgaatgaa ttgtttgaat cttgacaagt ctaacattcc
     4321 acccattcca aaaactctat ttttttcaaa acctgaagtt ttgaatgtta tatacgccat
     4381 tggatctaat ggattggtta ctggaattat caatgaatta tctgcatatt tttttatatt
     4441 tccaacaaca ttttttacta ctcctgcatt aattttcaat agatccattc tagtcattcc
     4501 tggttttctt ccagcaccag caactacaac aacaatatcg gaccctttca tatcttcata
     4561 attattagat cctttgatct caacatctat cccttgttca gatagtagat gattaagatc
     4621 catagcttct ccctgtggga gtccttctgc aatatctaaa agaagaattt gatcatctaa
     4681 ttgcttgatt gcagaacata gtgcagcaga tccaccaact tttcccgcac caattatagt
     4741 tatcatagaa tgcactcaga tttttaataa ttaaatctat gcagtagatg actctaaata
     4801 gaatttataa gcatttcaaa gaatttagtt ttatggtact tgttattgat tcacagattg
     4861 ctggtatttc cggtgatatg ctactttgca gtttggtaaa tatgggtgca aacagatcaa
     4921 agatcataga tggcattcgt aacgctgaat cactatgtaa ggatgtaaaa gtaaaaaaag
     4981 tagagtttgt agaagtgaaa aaaaattcat tacaggcaac tgcattacta ttagaaatag
     5041 atgatgacgt acatgagaga aaaggcatag agataaagga aataattgag aaatctgcag
     5101 aaaaattaac aatttctgaa agtgcaaaaa cttttgcaat aaaatcaatt gaaactctca
     5161 tacgtgcaga atcaaaaatt catggtgaac cagagaattc ggtgcacttt catgaggcgg
     5221 cgagttttga tacggttata gatatcctag gaactgctat tgctctagat gatttgggtt
     5281 gttttgatga tgatatagtt ggtacaccag tggctatagg tggtggcaca atgatctttt
     5341 ctcatggtat tagctccaat cctgcttatg caattttaga aatattcaga gaatcaggaa
     5401 ttattacagt aggcggaaat gtgaaggatg aaatgacaac ccctacaggg gctagcatgt
     5461 tagttaatct gattaaagaa tgctctgagt tttatccacc tatgaaaatc caatctattg
     5521 gttatggtgc gggccaaaaa gattttgaag gattttcaaa cgtattgaaa atagttagag
     5581 gagttgtatc aactaaactt cagcttgata cagtaaaaat acttgaaaca aacgttgatg
     5641 atgtatcagg cgaagtactt ggaaacatga ttgaaaaaat aatggcttat ggagcaaagg
     5701 atgtcacaat atcttctgca atcacaaaaa agggtcgccc tacaaattta gtatctgtta
     5761 tttgtgattc tgataccatg aattctatta tggatttgtt ggttactgaa actggaacac
     5821 ttggtgtaag agtaagaact tctgagcgat acatcgttcc tagatctgtt aaaacaatct
     5881 ctgtgaatat taatggacag agttttgata tcagatttaa aatcagagat ctaaataatg
     5941 actctcattt caaaattgaa tctgatgata tcaaagaaat ttctggtgtt cttagtattt
     6001 catttaagga gacagaagaa ttactaaatc aggaaattag gaaaaaacta tgaaaacaca
     6061 tcttactaag ttgctaaatt ggtttgatga caaaaacaac gtacttgtgg cactttcagg
     6121 tggtgttgat agtgctttgg ttgcttattc tgcttatgca aaattgggca aatcagcgat
     6181 agcagtaact gctgattata agacacttgc acaagaagaa ttagaatatg caaaaaaaat
     6241 atcttcagag attggaatca agcatttagt aatagaatac aatgagcttg ataacgaaag
     6301 ttttgttaaa aatgataaca atcgttgttt ttactgtaga aacgagcttt cggaacatct
     6361 cctagagata gcaaaagaat ctggatctga tattattgta gatggcacaa atactgatga
     6421 cttgggagat tatcgtccag gagtattagc gctgcatgag aatggaatta ggagcccatt
     6481 agcagaaact aaattctcaa agaaaatggt aagagaagta gctaaaaatg tgggcctatc
     6541 agtttatgat aaaccatcta attcatgttt agcatcacgt attccttggg ggcaaagtgt
     6601 aactgctgaa agattagcta gaattgaagt tggtgaaaaa attgtgaagc aacttacagg
     6661 agctaaacaa gtacgtgtta gagacatgaa tggcatagca agaattgagg ttggcgtaga
     6721 tgaactatct ttacttgaaa ataatttggt attacagaaa atcaaagacc aactaaaact
     6781 gataagcttt gacgatattt ctatagatcc agaaggttat agacctggaa aaattaacgt
     6841 gataactaat tgatctatct cgataatgct gcttcaacgg aaatacatcc agaagtagtt
     6901 aaggagatgt taccatattt tgacaaatat tatggcaatc cgtcatcaat tcatcaattt
     6961 ggtaggaaag caaagaatgc aatagaaaaa gcacgaaaac aagttgctgc acttattggt
     7021 gcagaaccaa atgaaatcat atttacatca ggtggaactg aatcaaataa tactattttc
     7081 tatggtataa tatcccaagg agagaaattt gagctgagtc atatcataac atcatcgata
     7141 gagcatgatg cagttttaga acccataagg caatttgaaa aaaatggatg tcagattaca
     7201 tatcttccag ttgataaaca tggaatgata aatctggatg atattaccaa atcaataagc
     7261 aagcaaacag ttatgatcag catcatgttt gcgaacaatg aagtaggcac aatacagccg
     7321 attaaagaaa tatcaaaaat ttgtaacaaa tatcaaatac cattacatac agatgctgta
     7381 caggcagtag gtaaaatccc aattaatgta aaagaattag gtgttgatgc attatcaatt
     7441 tcttcacaca aaattaatgg tcctaagggt gttggggcat tatttctaaa aaaaggttcg
     7501 acaacaactc catacattgt agggggtggt caggaaaatg ggatgcgttc tggcacagaa
     7561 aatgttgcta gtatagttgg atttggaaag gcatgtgaaa ttgcaaaaga taaattatct
     7621 gataacattt cccactttca aacccttcac tcttcaatgc tctcaagaat agtcaaagaa
     7681 atacctcatg taaaactgaa tggtcatcca caaaatagga tattcaataa tattcatctc
     7741 acatttttag gaataaatgg tgaggattta atcataaaat tggatgaaca tggaattgct
     7801 gcatccacgg ggtctgcatg ctcagtgcat actcaaaaag catcacatgt attgaaagca
     7861 atgggattta atcacgagca gataactggt tcattgcgta tatcatttgg atatacgaat
     7921 acattggatg aagtagagca aacagtagaa gttttaaaaa aagttgtatc tgaactgcga
     7981 agtgtttctc cctataaaac taaatataat ttttaaattt ctaattatgt acaagtgaaa
     8041 atttattttt tgataatata ataactgaga ttatggatat taatagaatc ataattgcta
     8101 tagttccaaa ctcaggaatc acataagttc ctattatctg tattttataa tcaccttcca
     8161 caaaaggaat tttaatagtt cgatattcag cattagtttg tgtttcttca tattcaactt
     8221 cgaatgtatc tcctgagata ttggtaatta aaacgataaa tacatcatca cttccctcag
     8281 ttgttttagc atcaaaactg tctctgggta atgataaaac aagatcacca gcagcatcaa
     8341 tgttgaaatc agtaacaagt gaataattat ctttatcaat ctgaatatct gtaacttctc
     8401 ctgttgttat agcatagcca acatcaaaga cttcagatgc attaggaatg ttgacagaaa
     8461 gagttgagga aggttgagta gtttgttgtg tattataaaa ttcaaaattt gtttctgtta
     8521 cgatttcttc tgtataaaac actctaacta aatacgtacc atttgttttc cataattttc
     8581 cagaagcaaa aatactttcc gcatacgttc catcgttagc caattgaggc tgagcaacat
     8641 ccacaaggtt tccactatga tagatttgta tggttatact aggtaaccca cctatcattt
     8701 ttttgacttc tccaaaaact actatggtgt caccctgtgt gtatgtttct ttattgagac
     8761 taactgttat tgattctgag tcctgtgcaa aaacaggaat tatggagata gtgataatca
     8821 ttatagccaa aatggaagaa attgccttat agtacacgat tgcattaaat atgaattcta
     8881 tatttccttt actgatttta taaatgaaca aaaaagattg aatgttacta gtatcttggt
     8941 actagactga gttttgtttt tgcagatagt gcaattattg acacaattgc tacaacaagt
     9001 atcattattg ctattgtacc aaattctgga attgcccaac caccaaatat tgtgattttt
     9061 tcggtgtctg cactgattgg aattgacaat tcattcacat tttgtacaaa atcctgactt
     9121 tcttcactgt ttactgttac aaaaaatgtc ccatcatcaa acggtttgat aatgtcttca
     9181 tgaagggtta tatctaatat tccatcatcg gttgcttcga tgtataacac aatagaattt
     9241 tcatcaccat ttacaaagaa tgaggtaata acaccaccgt caatcgtcca gttaatctga
     9301 ccatccttga ttgcatcttc aagtgttgtc tctgcagttt ccattgtatc tgaagtcatt
     9361 gcagattcta tagcattagt aagttctatt gatattgtag tttctacaaa accataagat
     9421 gcttgaattt ggtatgtacc atcaaatctc atcatagcac ctgcagtatt gaatgtggtt
     9481 gaaaatacac catcagaatc tacactaact tgagctatat ttgcaatatt tccatttgga
     9541 cttatgacca tgataacaac cggtacttca ttttgatcaa ccactgtaat atgtcctgtt
     9601 agaattatag ttgaagagtg attgtataca tcattgtcag tttctattgt aataccatca
     9661 tcagcataaa tgggttgtag tgcaagactt gcactagcta tcgccattat tccaatcaaa
     9721 atgaataatc ctttacgaat catagattaa acaagagtat tttcgtattt atacatattc
     9781 agaaaaataa taaaacaaaa aaaacaaaat aaaaagattg aatgttacta gtatcttggt
     9841 actagactga gttttgtttt tgcagatagt gcaattattg acacaattgc tacaacaagt
     9901 atcattattg ctattgtacc aaattctgga actacatgtc catctttgat atcaacttga
     9961 gtagatgttt tgtataatgc agcttcacct tgttgtgctg atattgtata catgccatct
    10021 tgactccata acgcacctcc aactgaaatt gtagtcatat aactaccacc tgaaggactt
    10081 acttgagcaa cagaaactat gtttccattt ggcgctgtta cagttaaaac gatatcacca
    10141 gttctgtctg ttgaaccact tacatctact gtagttgcac cctcaattgc atctgcggtt
    10201 attgttagtc cacgtatact tgcagaacca acttcttcta ttgtaacatc agctgctgtt
    10261 attgttgttg cagaaactga tgagtctgaa gtaccatcac taacatcaac gtcaactgat
    10321 acagagtact ttgatgagtc accttgattt gctgtaattg tataagatcc atccaacatg
    10381 cttacgttaa atgatgcaga gtaactacca cctgaaacag atgattgtgc aacaccatgt
    10441 attcccatac tgtttgataa tacaatagtt acatccaagt cacttcggtc agaatgtcca
    10501 cttatggcaa ttgtatcgcc agaaccatct gcagataatg acaatcctga tgccgcatag
    10561 gcattttgac ttagtgcaga aactgagatc atagctactg caagaaatac tattgatagg
    10621 gacgtcatcg tatatctacg agaattcatt attgagtctt attttgagtc ctatttatgt
    10681 ttcatgattg gatcgttttt ttctgtaatt tgagaaaaga accaacagaa aaggacggtt
    10741 tatccagagt aaatatatat taccttcaaa tcgctttttt tctgtttgtg ttgtttgcta
    10801 ttattcccac ttgatgttcg gtttggaggt tttctgtaat ggtatctaaa aagaacattg
    10861 tcctagtacc agatcacatt cttgtcccaa aacatgaaat catggctaaa aaagatgctg
    10921 aggaagtttt aaaaacatat aattgtcgta caactgattt accattaatt tttgtaaacg
    10981 atcccgctat cgttggactt ggagtaaaac caggtgacat gataaagata acccggaaga
    11041 gtgcaactgc aggagagagc ttctattaca gatacgtggt tgaagtatga ctttttcctc
    11101 ggataagcgt tggccagtaa ttcagaatat tctgatcacg caaggaatag ctaaacagca
    11161 tcttaactcg tttgatgaat ttttagagaa tggtctacag gaaattatta atgaagtaca
    11221 acaaatagaa attgaaaatg cggaatatcc ttacacaata aagttaggta aaataaaatt
    11281 tcaagaacca agaatgatgg agcttgacgg ttctatcact catactactc cagcagaatc
    11341 acgtttacgt aatgtttcat acgttgcccc tttacagatg gaagcaagtg ttgttgaaga
    11401 cggaaaaact ctagaatcaa aaccaattca tatcggcgat attccagtaa tggtaaaatc
    11461 aaatgcttgc atcttacgaa gttttacgga tcaaaaacta attgatcatg gagaagatcc
    11521 agaggatcct ggtggttatt ttatcattaa cggctcagaa cgagtgatag tgggattaga
    11581 agatttgtca tacaacaaga ttattgttga tactgacaaa gtgggtggta agtatataca
    11641 caaagcaaaa gtttattctt caattgttgg ataccgtgca aaattagaat tgattttaaa
    11701 agatgatgga ttaatcgttg caaaaatacc gggttctcca gttgatatac cagtaattac
    11761 attgattcgt gctcttggat tagaaactaa tgatgaaatt attaatgcag tatctcttgt
    11821 tgatcaaatt caggctgaac tggaaagttc ttttgaaaaa actgaagtta acacatcaaa
    11881 ggatgctatt gtttacatta gtaaaagaat agcacctgga atgttagagg aattccaaat
    11941 caaacgagca gaaacattgt tggattgggg tttacttcca cacttaggta aacatcctga
    12001 aagcagaaga gaaaaggcac agtttttggg tgaagctgct tgtaaattac ttgaattaaa
    12061 actgggatgg attgaaattg atgataaaga tcattatggc aacaaagtca tcaaatttgc
    12121 aggacaaatg ttagcagatt tgtttagaac tgcatttaga aatttagtta gagacatgaa
    12181 atatcaactt gaacgatccg gacaaaaaag aggtattaat gctgtagctg ctgcggttcg
    12241 acctggtatc atcagtgata aactaaacaa tgcaattgct acaggtaatt ggggacgtgg
    12301 aagagttgga attacacaat tacttgatag aaccaattat ctttctacaa ttagccatct
    12361 acgacgagtt cagtcaccat tgagcagaac ccaaccaaac tttgaagcaa gggatctgca
    12421 ttctacacac tttggaagga tttgtcctag tgaaacacca gaaggctcta actgtggctt
    12481 agtcaagaat ctagcattat ctgcaataat ttctactagt gtggatagtt cagatatcag
    12541 acaaaaatta gatgatgaag gggtagcaga ttttcttgat gttaatgact ctgtgaaaaa
    12601 aaccggtgcg agggttttcc ttgatggaaa atttattggt tatttcccag atggcgagaa
    12661 actagtttta acattacgtg aactacgtag aaataataaa attaattcac atgttggaat
    12721 atccttgcat actccagaag aagagggtgc tacaaagaga ttatacgtaa attgtaatgc
    12781 aggacgagtt ttgagaccag ttattttgat taaagatggt aaatcactgc taacaaacga
    12841 tcttattgaa aagacttcaa aaaaacttgt ttcttggctt gacctaattc gtatgggtgt
    12901 aatcgagctt atagacgcaa atgaagaaga aaattgtgat atcacatttg atgaaaaaaa
    12961 cacaaagaaa tttactcatc tagagatatt ctcatcagcc atattaggtg caggcgcttc
    13021 cattatccca tatcctgaac ataaccagtc tccacgaaac acatacgaat ctgctatggc
    13081 aaaacaaagt cttggttttt caacaccaat gatgaatact agtacatatg tcagacagca
    13141 ctttatgcta tatccacaaa caccaattgt ttccacaaag gcgatgaatc ttcttggttt
    13201 agagaaaaga cccgcgggac aaaattgtgt tgttgctgtg ttaccttttg atggatataa
    13261 cattgaagat gctattgtat taagccaatc atcagtagat agaggactgg gaagaacatt
    13321 cttttataga atatatgaat cagaggcgaa acaataccct ggaggaatgc gtgatagttt
    13381 tcaaattcca aatgcagatg aaaacattag agggtataaa ggtgaaaaat catatagatt
    13441 attggaagaa gatggaatag ttgcaactga atccactgta aagggtggag acattctcat
    13501 cggtaaaaca agcccaccta gatttatgga agattatcgt gagtttgaat ctcagggacc
    13561 ttatagacgt gatacttctg taggtgttag accaactgaa aatggagttg tagacactat
    13621 tgttatgaca caatcaaacg aaggaggaaa aatgttcaaa gtaagagtaa gggatatgcg
    13681 tattcctgaa attggagaca aatttgcttc aaggcatggt cagaaaggtg ttttgggtat
    13741 tttagcaaaa caagaagatt taccatatac tgctgatgga atttcgccag atgttctaat
    13801 taaccctcat gcatttccat cacgaatgac agttggtatg atgatggaat ctataacagg
    13861 gaaagctgcg gctctacgag gagcaaaatt tgacggctct gcctttgtag gagaaaagat
    13921 agaccaagta agagacgtca tgaaagatgc cggattcaaa tattctggta aggaaataat
    13981 gtatgatggg agaactggta aaccattccc tgttgaagta tttattggaa ttgtctatta
    14041 tcaaaaacta catcacatgg ttgcagataa gatacatgca agggcgcgtg gacaagtaca
    14101 aatgctcacc aaacaaccaa ctgaaggtag agcacgtggt ggcggtttac gattcggaga
    14161 aatggaaagg gattgtataa tagcttatgg agcatctatg attcttaaag atagattact
    14221 tgacgaatcg gataaggatg atatttttgt atgtgaacga tgtggacttg ttgcgtatca
    14281 tgatattaaa caaagaaggt atatttgtag agtttgtggt gataaaggaa aagtatcttc
    14341 tgtatctgtt gcatatgcgt tcaaattatt attacaagaa atgcaaagtc tgaacattgc
    14401 gccacgtctg atgataaagg agaaaatata atgtctattg aaacaataaa atctattcaa
    14461 ggaatcaggt tctctgtttg gtctccaagt gaaattagaa aatattcagt tgctgagatt
    14521 actgctcctg aaacttatga tgaggatgga atgtcagtac aaggaggttt aatggatgga
    14581 cgacttggaa ccttggagcc tgggcaaaaa tgtttgagtt gtggtaatac ttctgctaga
    14641 tgtcctggac actttggtca catagagttt gccgaacctg tacttcatat agcattcatt
    14701 gacagtattc acaaactatt attatgtaca tgtagaagct gttcaagatt aaaaattcct
    14761 agcgatgatc ttgaaagact tgcaaatatg aaaaaacaaa aggcgtcata tactataatc
    14821 tcccagaagc gtgtccctga acaaatttta gaaaaagcaa aaaaacaaaa ggaatgtccc
    14881 cattgtggaa aagtccaata tgatttaatt tttacaaaac ctacaatttt tatagaaaaa
    14941 actgaattag gagaaaacag attacttcca atcacaataa gagagcgatt ttcacaaatt
    15001 actaatgatg atcttgattt attgggatat gatcatacta ctgctaggcc agaatggttt
    15061 gttttgcaag tattacctgt accacctgtt acggtaagac cttcaattat acttgaaacc
    15121 ggaatcagat cagaagatga tttgacacat aaaatggtag atattatcag agtaaatcaa
    15181 cgacttaaag aaagcaagga agctggaaca ccacctttga tcgttcaaga tcttgttgat
    15241 ttattgcaat accatactac aacttatttt gataatgaag tttcaggcat tccgcaggca
    15301 catcatcgtt ctggtagacc actcaagaca ctaacccaaa gattgaaagg caaggagggt
    15361 agatttagag gttcattatc tggaaaacgg gttgatttct caagtagaac cgtcatctct
    15421 ccagatccaa atttggattt gtcagaggtt ggcgtgccag tacctattgc tatgaaactt
    15481 acaattccag agattgtaac agaatggaat attgaaaaac tgaaaaaact aattattaat
    15541 ggaccaagta catttccagg cgtaaattat gttgttagac ctgatggtgt aaaaattaga
    15601 cttgattttg tagaagatag atcaatcatc gcagacagta ttgaaacagg atacctcata
    15661 gaaaggcatc ttctagatgg cgatattgta ttgtttaaca gacaaccatc actccaccaa
    15721 atgtccatca tggctcatca cgttcgtgtg cttcctggaa aaacttttcg attacatcct
    15781 tccgtttgcc caccatacaa tgctgacttt gatggtgatg aaatgaatct tcatgttcca
    15841 caaagtgagg aagctcgagc tgaagctata ctattaatgc gagtacaaga acaattaatt
    15901 tcaccaagat ttggcggtcc tataattggt ggtttacgtg actttatcac tggagcatat
    15961 cttctaacta aggatgatac aattctaaca agccaagaat ttagtaatta tgcgatgtta
    16021 ggtggttata aaggaaagtt acctgaacct tcatctaaaa ataaaactgg agcatcttat
    16081 actggcaaac aactgttttc tatctttctt ccttctgatt ttaattatat tatgacttca
    16141 aaatggtcaa aagggacaaa gggaacaaat caagatgttg taattaaaaa tggtgagcta
    16201 attagtggtg tcatagacaa atcatctata ggtgcagaag aaccagaaag tgttttgcat
    16261 agaattgcaa aagactatgg taatgaaaga gctaaaaact ttctaaactc ggttcttatc
    16321 attatcaaac aatttatcac tgattatgga tttagttatg gttattctga tttggaactt
    16381 tccgataaag atagggaggc aattcttgat gacatccaag caacatatga caaggttggt
    16441 gatattatca ccgaaaaaaa taagggaact ctaaaaggat tgaggggaat gactgtttct
    16501 gaaacagctg aggctaaaat tacatttgag ttagcaaaag ccagagatcg tgcaggtatc
    16561 actgcaaatt caaatcttga agataataat gcaggaaaaa taatggctac tactggtgca
    16621 agaggttcag cattgaatgt aggtcagatg gcgggtgcat taggtcaaca atcaagacgt
    16681 ggaaaaagac tacatactgg atatgctaat cgtactttac cacacttcaa aacacatgat
    16741 gaaaatcctg actcgcatgg atttgtcaaa tctaatttcc gagacggtct ttcaacatta
    16801 gaatttttct tccatgctat gggtggaaga gagggtcttg ttgatactgc agtaagaaca
    16861 caacaaagtg gatatatgca acgtagactg atcaacgcgc tagaacatat caagttagaa
    16921 tatgatggaa cagtaaggga tccacataga catattatac aatttttgta tggggaagac
    16981 ggaatagacg ttgcaaaaag tgatcatggt gaagccttta acattaatag attaatagaa
    17041 tcccaaagaa tcatagattc tggtaaagct gcaactaagg aggaaattac atcattaaca
    17101 aaaaaatatt ccaagacatt caacccccga ttaacttcca tagtaagcga tggattactt
    17161 gattctaaac taagtaaagt gggaatagaa agtgtttgta agaagggttt agaattatat
    17221 gataactgta aagctgaacc tggtcaggca gtaggcattg taactgcaca atcaattggt
    17281 gagccaggaa cacaaatgac attaagaaca tttcatactg caggaattgc agaaaagaac
    17341 gtaacacttg gtttaccaag aatcattgaa ttagtagatg cacgtaaaaa acctgctact
    17401 ccatctatgg atatatatct agataaagat tcaaaagttt ctcaagaaaa ggcagttgaa
    17461 gtagctcgtg atatcaaaga aacaaaaata tctgatttgc ttataagaac agtaagtgat
    17521 catgcaactg agataaaact tgaatttgat atgaagaaac tagaaactag acatgtaaca
    17581 cttgagcagg tcacaagtct attagaatca aataaaaaat tcaaactcga atttagtaaa
    17641 aatactataa ttttgaaact aattgaagaa tcagattccg tgactgttaa tacaataaaa
    17701 aataaagtgt taaaaagtac tgtaagagga gtatctgaca ttgaacgtgt taccctcaaa
    17761 catgataatg gtgaatggat tattcagaca actggttcaa atgtggcaaa aatattacat
    17821 gtcaaaggaa ttgataaaca aaatattaga acaaataatg tgtatgaaat tgcagaaacc
    17881 ttaggcatag aagcagcaag aaactctcta atcaacgaat taaaatttac cctcgaaaat
    17941 caaggtttag aagtcgatac acgatatctt atgttagtat ctgatcttat gtgtcataaa
    18001 ggatatctgc aacagattgg tcgtcatgga atagcaggtt caaaggatag tgttcttgct
    18061 agagcagctt ttgagattac tgtgccaaca attgcgggtg ctgctaaact aggacaaata
    18121 gaagaattaa aaggaattac tgaaaacgtt attgttggaa gccaaatacc tattggtagt
    18181 ggcactgtgg acatttacat ggaatctgca actaaaacat aattattaat tttttaatta
    18241 taaaatacag aatattattt ggtataaatc tataaaggga taataaaaca gacaaacaag
    18301 aaaggtataa cgatggcaaa acttttagaa aaaactctca aagacgcaat aaaagaaaat
    18361 aatcatgtaa ttggaacaaa acaagtaatg tcttctataa aatcaagtaa actggttata
    18421 atatcgcaat ctattccttc tcctactatt caacagatag aagacaatac aaaaaaggaa
    18481 aatataccat tactacattt tgaaggaaca tcaattatgc taggaaaact ttgtgggtta
    18541 caatttagag tatcaacaat ttcacttaaa tcgttatcta acacaaatat acaagcaatt
    18601 ttaaaagaat ctgaaacaaa atgattttac aagaaaaaga ttattatcta agtttaaaag
    18661 agatagaaaa acagccaatc taaaagataa acaatgacaa actcaataaa attaactaca
    18721 gaacaaatgc gtttcatctc gctttttcaa aatgttacaa aatcttctgc acgtgattgc
    18781 ttagatgatg aaaaacagga tagattaatt tttgttgtga atgagggcaa aatggggctt
    18841 gctatcggca aaggcggttc taatataaaa tcacttcaaa atattctaaa acgaaacgta
    18901 gaattaattg aatataatga ggaaccaata aaatttttga aaaatatact gaacgcaaaa
    18961 ttaatcagtg aaataaaatt aaatacaaaa cctgatggtt cattacatgc tgtagttttt
    19021 gttgatcctg ggaaaaaagg tatagtggtc ggacgtgaag gacgtaatgc agaaaaggct
    19081 agactgctag ctaaaagata ttttaatatt tcaactgtca tgataaatag tcaagaaatg
    19141 acacaacttg aggtgtaatg tttggctaaa tcacctcttg gattatttgc tggccgtgtg
    19201 ctaaaagaaa agaagaaacg tcagcgttgg tctatatcta gatacaaacg ccgagaactg
    19261 ggtttggata aaaaatcaaa tcctttgggt ggtgctccac aagcaagggc aattgtttta
    19321 gaaaaagtcg gtgtagaagc aaaacagcca aactctgcag ttagaaaatg tgttcgtgta
    19381 caattaatta aaaatgggaa atcagttact gcatttttgc cacgagatgg tgcaatgaac
    19441 ttcattgatg agcatgatga ggtacatctt gaaggtatgg gtgctactca gggcggtgct
    19501 atgggtgata ttccgggtgt acgatacaaa gttttcaaag ttaatggaac atcgttaaag
    19561 caattagtaa caggaaagaa agaaaaaccg agaagatgat aaaatgactg aacaaaaaat
    19621 gttgcttttt agaagatggg atttatctac aattgaaatc aaagattcgg gtctaaaaaa
    19681 tgctatatgt cttaaaccat caatacttcc aatagatttt ggacgatctg ccctaagacg
    19741 attcaacaag gctgatgtta acatagttga acgattagta aacaaattaa tgcattttgg
    19801 caaaaaatat tcaaaaaaca caggtaggat ggctggaaaa aaagatcatt catttaatgt
    19861 tgtcaagact gcttttgaga taattcatct aaaaacagaa aaaaacccaa ttgaggtttt
    19921 agttcgtgca gtagaaaatt cagcaccaaa tgaagatact actagaattg tatatggtgg
    19981 aacagtatat catgtatcgg ttgatgtggc accaatcaga agagttgatc tggcattacg
    20041 atttattgca gatggtgtca aagaagctac attttccaac ccaaaaccaa ttgaagaata
    20101 tttagctgag catctaattc ttgcagcaaa caatgatcct acatctccat ctgttaagaa
    20161 aagaaacgaa ttagaacgta ttgcacaagc atctagataa ttaaaccaat agcccggccc
    20221 ggactcgaac cgaggtcaaa ggctccaggg gcccctatgc ttgccactac actaccgggc
    20281 tacaagaaca tttgtaaaat atagaatata attatttcat tctaacttca ttcattgcag
    20341 ttgaataa
//
LOCUS       DASCPJ010000061         9122 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000976513, whole genome shotgun sequence.
ACCESSION   DASCPJ010000061 DASCPJ010000000
VERSION     DASCPJ010000061.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000976513"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(52..660)
                     /locus_tag="QGF01_02785"
     CDS             complement(52..660)
                     /locus_tag="QGF01_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19856.1"
                     /translation="MKSVASAPAKIILFGEHFVVHGTKAILAAIDKRVTVTSSFTENK
                     TIKINSQLGTLEVPMSSSHEEVKSELRPFVYLANKMINSHQDVNGLEITVNSDIPIGV
                     GLGSSSACCVAATASISGLFNELSREEIINLSIEAEKTIFPDTSGADCTVCTYGGKIV
                     YPSIEKIGNTFDLNLLIANSMIPHSTKSSVEKVNKFKENDER"
     gene            complement(682..1287)
                     /locus_tag="QGF01_02790"
     CDS             complement(682..1287)
                     /locus_tag="QGF01_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HJN19857.1"
                     /translation="MSQIAEQPEMKDKIIASGIRVGTAVKTKFMIPFITQSNPEGFYL
                     LDLDLTLNRIQTAGRFIKKFDMSKVIVCSGSNLSKTAIEKFCEITGAKMMFGRFMPGT
                     LTNPSLPFYSEPQLVIISDPQIDTQAVTEATNAGIPVIGISNTDNITSQLDLIIPANN
                     RGRQSIATIYWLLAGEIDPSIKAKYQIEDFETKTVDTEEEL"
     gene            complement(1293..2531)
                     /locus_tag="QGF01_02795"
     CDS             complement(1293..2531)
                     /locus_tag="QGF01_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HJN19858.1"
                     /translation="MAKITSINGRLIYNSRGSKALEVDVVSDNQYLGRSCAPSGASVG
                     KYEAVSFPNDQPEESLRFLDEISKKIIGYESDDLKFIDEVIKSVDGTADYSKIGGSLA
                     YAITIATMESASKAVNKQLFQLIGEQNEYRFPLPLGNILGGGAHAGPGTPDIQEILIC
                     ATGSKTIRDAIETNFAVHKELQKVIEKVDPTFTNGKGDEGGWAPKCKNEKALEFCVTA
                     CENLGYTLGKEVSLGVDFASSTQWNEEKGKYVYERAGFENTPEKQIEFVTGIIKKYKI
                     IYAEDAVHEEAFEDMAELTAKFPDTLIVGDDLTVTNKNILKKAIEQKSCNAAILKVNQ
                     AGSLYDALKFAKLASSNNIKLITSHRSGESIDSQISHIGIGTNSKMLKIGVVGGERVA
                     KINELIRISEHDLIQGVMEI"
     gene            complement(2533..2769)
                     /locus_tag="QGF01_02800"
     CDS             complement(2533..2769)
                     /locus_tag="QGF01_02800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HJN19859.1"
                     /translation="MLIPVRCFTCGNLIADKFDEFHNAIKSGEDAAKTLDSLGIERYC
                     CRRMLLTTLETIQQVVPFYEAIHQRNLEIKSELE"
     gene            2818..2902
                     /locus_tag="QGF01_02805"
     tRNA            2818..2902
                     /locus_tag="QGF01_02805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2854..2856,aa:Leu,seq:gag)
     gene            complement(2911..3519)
                     /locus_tag="QGF01_02810"
     CDS             complement(2911..3519)
                     /locus_tag="QGF01_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HJN19860.1"
                     /translation="MGLKNVKSSLPLISKELEKTNSSREFLIKNNRQVIINCSQAIIS
                     IHKNDLLSAKNKIKTAKEQLVKLKQKTDGNLNRYIITPEQELVEAVSLLSIIEGKDIP
                     SYKTLKVSAESYILGLLDCIGELKRQVIDQIRIGNSKNAEKLFDIMESLYLMLYPFAT
                     FDKIVKESRRKLDVNRMLVEETRLVLTEEIRRQEFLKKMNSK"
     gene            3592..4239
                     /locus_tag="QGF01_02815"
     CDS             3592..4239
                     /locus_tag="QGF01_02815"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HJN19861.1"
                     /translation="MEISVDQMYQIENKGHDMGFFKKFMMENAGAASVKRLAEKLENL
                     QSKQILIFVGLGNNGGDGLVMARHLAGYDAKVTVMLLGTPDKIKTEESNWNWSILEKM
                     PSIRLLTGNSIHFDFNPDIIIDSILGTGISGDIKEPYASAINYINKTNSYKFAVDVPS
                     GLDPQTGDTANICTKCDMTVTFHKMKKGIPKRKDLTGEIFVEKIGIPPEAEIGVL"
     gene            4236..4847
                     /locus_tag="QGF01_02820"
     CDS             4236..4847
                     /locus_tag="QGF01_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase family protein"
                     /protein_id="HJN19862.1"
                     /translation="MKVYQIPVGPMQNFSYIVEDESTHEAIVIDPSWDLEKLTEIISE
                     QNLNPKYIVNTHWHDDHTRGNEELAKELSVKIVQHDASQLKNDLIVSDGGSIKFGCSE
                     FAVYHTPGHSKDSICLVGDGKIFSGDTLFVGNCGRIDLPGGSAKELYHSLFDVITNFT
                     DDLVLYPGHDYGSSPTSTIGKEKQTNPVMQRISEAEFIQRMGG"
     gene            4865..5914
                     /locus_tag="QGF01_02825"
     CDS             4865..5914
                     /locus_tag="QGF01_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HJN19863.1"
                     /translation="MHCNEEKGKKFIKDVEQKRFLFSFVLSYTETCEIPGITVAGANT
                     DFIKFTPPADAEFLHYGYCKSIDVIPMTPDGKPTPALLTKASLESASIPQVIINAGSK
                     ISPKLPYFQTGITHGKNIAIEPAFEQSNVVQAIDYGRILGRTLASSTDCLIIGESVPG
                     GTTTALAVLKSLGVDAHVSSSMPENPQSLKNGVVAKALARSDSKEPYDVIANAGDPMI
                     PFVAGMLSTASKITNVILAGGTQMAAVLAIGKSLGYDQNKVALGTTSYVIDDKNANLL
                     DIIKSISDIPVLSIDPKLNDSKFDGLRAYSKGFVKEGAGAGGSMISSILKTGISSKKL
                     LELVDKEYTRITTSQ"
     gene            complement(5901..7655)
                     /gene="glmS"
                     /locus_tag="QGF01_02830"
     CDS             complement(5901..7655)
                     /gene="glmS"
                     /locus_tag="QGF01_02830"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HJN19864.1"
                     /translation="MCSIIGYSGLESAAPILVKGLQKMEYRGYDSVGIATESGKKIVL
                     KKGVGKVAEVNNSIMLDTLPGNIGIGHTRWATHGKVTEKNAHPHSSNSGKIAIAHNGI
                     IENFEELKSELQNKGYKFQSETDTEVIANLIQLNFDETADIKQSIMKTVNQLKGHYSF
                     VVIFEDGTLAAARFHEPLIVGVGKNSHYISSDVLGFIEKTDDAIYVDNEDFVIVDGSG
                     IHIFDFDGTSVKYQVTKVSKEFADVYKGDFAHYTLKEISEQPDTILRAVDNNQIEEFV
                     DNIKQAKNLYITGSGTSYNAAEITKYLMSKFAKIKINTLISSELPFSPNEIEPNSTLI
                     TISQSGESADVLEAVKIAKESNAKILSIVNLLNSSLSQQSSLAIGLNCGPEIGVAATK
                     SFTSQLSILYKICDQLCNGCINPDWEKVSSAISQILSNNSKIKEIAKSLKDVSDIYVL
                     GRGIHLPIAKEAALKLKELTYIHAEGIPAGELKHGPLALMDSNVYVIIINPNDSTYND
                     TLNSANQIKARGAKIIGISDKKSNVYDYWIDIPPIDEALYPLIEIVPIQLLSYYSALE
                     KKTDPDYPRNLAKSVTVK"
     gene            complement(7700..8350)
                     /locus_tag="QGF01_02835"
     CDS             complement(7700..8350)
                     /locus_tag="QGF01_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HJN19865.1"
                     /translation="MGDPKTPRRVWKKPKRPLNYDLMMDELKTLGTFGLKTKRELWKA
                     NTELSRVRHQARILLALRQEIRDEKEPILINSLSKIGLVNNDSTLDDVLNLQVTDLLS
                     RRLQTIVQKKLYFKTPYQARQAIVHGHVMIGDSVITIPSYTVKTDEEEKIHLITESSY
                     NQTLTKPSSDLGSPETENLEDNANEQSENEKPAAKQESDTEPAAKQESDTESTNES"
     gene            complement(8360..8989)
                     /locus_tag="QGF01_02840"
     CDS             complement(8360..8989)
                     /locus_tag="QGF01_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HJN19866.1"
                     /translation="MSTQYEHIVRILGNDIPGDKKIIVGLTQIRGVGYMFANTILNTL
                     KINPNQRMGHLSTDNIQLIEKIVKDPLSSDFPSWFLNRRKDLETGVNKHLVTSDIAFT
                     VRNDIEREKTAGSWRGYRHMYGLKVRGQRTRCTGRKGGAVGVAKGGKIMPSTGGGGAG
                     APAAEGAAAEGAAAEGAAETPVTNTKDEKPKTESKGTVEKKESAKPEKK"
BASE COUNT         2743 a         1641 c         1469 g         3269 t
ORIGIN      
        1 cagacataat tcaccctgct tcgtttttgt gtatttctca ttacccatca ttcaccgttc
       61 atcattttcc ttgaatttgt taaccttctc aacactactc ttagtagaat gcggaatcat
      121 agaatttgct attagaagat taagatcaaa agtattacca attttttcaa tgctaggata
      181 tacaatcttt ccaccataag tacagacagt acagtctgca ccggacgtat caggaaagat
      241 tgttttttca gcctctattg atagatttat gatctcttca cgtgatagtt cattaaacaa
      301 tcctgagatt gaagcagttg cagcaacaca acacgcggag gatgatccaa gtcctacacc
      361 aataggaata tcagaattta ctgtaatttc taatccgtta acatcttgat gagaattaat
      421 cattttattt gccagataaa caaatggtct gagctcactc ttcacttctt catgcgagga
      481 cgacataggc acttcaagcg ttcccaattg tgaattaatc ttaatggttt tgttttccgt
      541 aaaagatgat gtaactgtta cccttttgtc tattgcagca agaattgctt tagtcccatg
      601 aacaacaaaa tgttctccaa atagaattat ttttgctggc gctgatgcta ctgatttcaa
      661 tattttggtt tagaaaatga tctataattc ttcttctgta tctacagtct tggtttcgaa
      721 atcttcaatc tgatattttg ctttgattga tgggtctatc tcaccagcca ataaccagta
      781 aattgtagca atagactgac gacctctatt attggcaggg attatcaaat caagttgtga
      841 tgttatatta tctgtattag atattccaat tacaggaatt cccgcatttg tggcttcagt
      901 aacggcttga gtatcaattt gtggatcaga aattattaca agttgtggtt ccgaataaaa
      961 aggcaatgag ggatttgtga gtgttccagg cataaaccta ccaaacatca tttttgcgcc
     1021 agttatttcg cagaatttct ctatcgccgt tttagataaa ttacttcccg aacatacaat
     1081 cactttactc atatcaaatt tttttatgaa tctccctgct gtctgaattc tatttagagt
     1141 cagatctaaa tcaagcaagt agaatccttc aggattagat tgagtaatga atggaatcat
     1201 aaatttagtt tttactgctg ttcctactct tatgccactg gcaataattt tgtctttcat
     1261 ctctggttgt tcagcaatct gactcattga ttttaaatct ccattactcc ttgtattaaa
     1321 tcatgctctg aaatacggat caactcatta atttttgcaa cacgttctcc gcctacaacc
     1381 ccaatcttta gcattttaga attagttcca ataccaatat gagaaatttg cgagtcaatt
     1441 gattctccag aacggtgtga tgtgatcaat ttaatattat tagaacttgc taatttagca
     1501 aatttcaatg catcatacag acttcccgcc tgatttactt tcaaaattgc agcgttacat
     1561 gatttttgct caattgcttt ttttagtata tttttgtttg tcacagtcaa atcatctcct
     1621 acaattagtg tatcaggaaa ttttgcagta agttctgcca tgtcctcaaa cgcttcttca
     1681 tgtactgcat cttctgcata aattatttta tattttttaa taataccagt tacaaactca
     1741 atttgttttt ctggtgtatt ttcaaatcca gctctttcat agacatattt tcccttttct
     1801 tcattccatt gtgttgaaga tgcaaaatca actccgagtg aaacttcttt ccctaaagtg
     1861 tatcctaaat tttcacaagc agttacacaa aactctaaag ctttctcatt tttacacttt
     1921 ggtgcccatc ctccttcgtc accttttcca tttgtgaaag tgggatcaac tttttctatc
     1981 actttttgta gctctttgtg tactgcaaaa tttgtttcaa tagcatctct aattgtcttt
     2041 gaacctgttg cacaaattag aatttcttgt atatcaggag ttcctggtcc ggcatgtgct
     2101 cctccaccca aaatattgcc taatggaagt ggaaatctgt attcattttg ttcaccaatt
     2161 aattggaata attgcttgtt aactgctttt gaagctgatt ccattgtagc aattgttatt
     2221 gcatacgcta atgagccacc aattttagaa taatctgctg ttccgtctac tgattttatt
     2281 acttcatcaa tgaattttaa atcatcagat tcatacccaa taattttttt tgaaatttca
     2341 tccaagaacc gaagggattc ttctggttga tcatttggaa aacttaccgc ttcatatttc
     2401 ccaacacttg caccagaggg tgcacaactt ctacctagat attggttgtc agaaacaaca
     2461 tcgacttcaa gagctttact tcctctacta ttgtaaatta atctaccatt aattgatgta
     2521 atcttagcca agttattcta actcagactt gatttctaaa ttcctttggt gtattgcctc
     2581 atagaatgga accacttgct gaatcgtttc taatgtagtt aataacattc ttctacaaca
     2641 atatctttct attcccaacg aatccaaagt tttagcagca tcttcaccag attttattgc
     2701 attatgaaac tcgtcaaatt tgtcagcgat aagattacca catgtgaaac atctgactgg
     2761 aattagcata tttttggaaa acaatcagat tataaaaacc ctgcacgaaa actaattgcg
     2821 ggtgtcgccc agcctggcca aaggcgctag cttgaggggc tagtctctta ggagttcgtg
     2881 ggttcaaatc ccatcgcccg cataaatatg tcattttgaa ttcatttttt taagaaattc
     2941 ttgtcttcta atctcttcag taagaacaag tcttgtttct tcgactaaca ttctattaac
     3001 gtctaacttt cttcttgatt cctttacaat tttgtcaaag gtggcaaaag gatatagcat
     3061 caaatacaag ctctccataa tatcaaataa tttctctgca ttttttgagt ttccaattct
     3121 gatctgatct attacctgtc ttttcaactc tccaatacaa tcaagcagac caagaatgta
     3181 tgattcagct gaaactttga gtgttttgta tgaaggaata tcttttcctt ctattataga
     3241 taaaagtgaa acggcctcaa ctagttcttg ctcaggagtt atgatatacc tattgagatt
     3301 gccatcagtc ttttgtttta attttacgag ttgctctttt gcagttttta ttttattttt
     3361 tgctgaaagc agatcatttt tatgaattga gataattgct tgactacaat tgatgatcac
     3421 ttgcctatta tttttaatta aaaactctct tgacgaattt gttttttcca attctttaga
     3481 aataagaggt aatgatgatt tgacgttttt tagtcccatt ggttctgttt ttaatattct
     3541 cagttaaagg catttgccaa tactcttatt ttagatattt ttttgtgaat tatggaaata
     3601 tcagtagatc aaatgtatca aattgagaat aaaggacacg atatggggtt ttttaaaaaa
     3661 tttatgatgg aaaacgctgg tgctgcatcg gtaaaacgat tagcggaaaa acttgaaaat
     3721 ttacaatcaa aacaaattct gatttttgtg ggattgggaa ataatggtgg tgatggtttg
     3781 gttatggcaa gacatcttgc aggttatgat gcaaaagtta ctgtaatgct tcttggtacg
     3841 cccgataaaa tcaaaactga agaaagtaat tggaattggt caattttaga aaaaatgcct
     3901 tctatacgac tactaactgg taattctata cattttgatt ttaatcctga tattataata
     3961 gatagtattt tgggaactgg tatttcagga gacataaaag aaccatatgc gtctgcaatt
     4021 aattatatca acaaaactaa ctcctacaaa tttgcagttg atgtaccttc tggattagat
     4081 cctcagacag gagatactgc aaatatttgt actaagtgtg acatgacagt gacattccat
     4141 aaaatgaaaa aaggaattcc taaaagaaag gatttaactg gtgagatatt tgttgaaaaa
     4201 attgggattc ctccagaggc agagattggt gttctatgaa agtataccag attccagtag
     4261 gtccaatgca gaatttttca tatattgttg aagatgaatc tacacatgag gcaattgtga
     4321 ttgatccatc atgggatttg gaaaagttaa ctgaaattat cagtgaacaa aaccttaatc
     4381 ccaagtacat cgttaatact cattggcatg atgatcatac cagaggaaat gaggaattgg
     4441 caaaagagct cagtgtaaag attgttcaac atgatgcatc acaactaaaa aatgacttga
     4501 tagtttcaga tgggggttcc ataaaattcg gctgttctga atttgctgta tatcacacac
     4561 ctggacattc gaaagatagt atttgtcttg taggtgatgg aaaaattttt tcaggtgaca
     4621 ctttatttgt aggaaactgt ggtcgtattg atttaccagg aggtagtgca aaggaactct
     4681 atcatagcct ttttgatgta attacaaatt ttaccgatga cttagttttg tatcctggtc
     4741 atgattacgg ttcatctcca acatcaacta taggaaagga aaaacagaca aatcctgtaa
     4801 tgcaaagaat atctgaagca gaatttattc aacgaatggg tggttaatta tatctgatat
     4861 caaaattcac tgtaatgaag aaaaagggaa aaaattcata aaagatgtag aacagaagcg
     4921 attccttttt tcatttgtgc tttcttatac ggagacatgt gaaattcctg gaattactgt
     4981 agcaggagct aataccgatt ttattaaatt tactccacct gcagatgcgg agtttcttca
     5041 ttatggatat tgtaaaagta tcgatgtaat tccaatgacg cctgatggaa agccaacgcc
     5101 cgcattgttg actaaggcat ctttggaatc agcaagtatt ccacaagtaa ttattaatgc
     5161 tggaagtaaa atttctccaa aacttccata ttttcaaact ggtataactc atggtaaaaa
     5221 tatagccata gaaccagcat ttgagcaatc taatgttgtg caagctattg actatggcag
     5281 aatacttggt agaacacttg cttcatctac tgactgtctc ataattggtg aaagtgttcc
     5341 tggtggtaca actactgcac ttgctgtttt gaaaagtttg ggagttgatg cacatgtgag
     5401 ttcaagcatg ccagagaacc cacaatcatt aaaaaatgga gttgttgcaa aagcattagc
     5461 acgttctgat tctaaagaac catatgatgt gatagccaat gcgggtgatc ctatgatacc
     5521 ctttgttgcg ggtatgctaa gtacagcatc taaaattaca aatgtaattc ttgcaggtgg
     5581 aactcagatg gctgcagttc ttgccatagg aaaatcgtta ggatatgatc aaaataaggt
     5641 tgcattagga actacgtcat acgtaattga tgataaaaat gcaaatttat tggatataat
     5701 aaaatcaatt tctgatattc ctgtattatc aatagatcca aaattgaatg attcgaagtt
     5761 tgatggttta cgtgcttatt caaaaggatt tgtaaaagaa ggagctggag ctggtggcag
     5821 catgatttct tcaattctta aaactggtat atcatcaaaa aaattattag agttggttga
     5881 taaagagtat acacgaatta ctacttcaca gtaactgatt ttgctaaatt tcttggatag
     5941 tcaggatctg ttttcttttc taatgcagag taatatgaca gcaattgaat tggaacaatt
     6001 tcaattaagg ggtatagcgc ctcatcaatt ggaggtatat caatccaata atcataaaca
     6061 ttactttttt tgtctgatat tccaataatt tttgcacctc tagctttgat ttggttggca
     6121 ctgttcaatg tatcattata agttgaatca ttcggattaa ttattatcac atatacattt
     6181 gaatccatta gagctaatgg accatgtttt aattcacctg caggtattcc ttcagcatga
     6241 atgtatgtaa gttcctttag tttgagtgca gcttccttag caattggaag atgaattcct
     6301 ctcccaagaa cgtaaatatc tgatacatct ttaagacttt ttgctatttc ttttattttg
     6361 gaattatttg atagaatttg tgagatggct gacgaaactt tttcccagtc tgggtttata
     6421 caaccgttac atagttgatc acagattttg tataatatag ataattgtga tgtaaaactt
     6481 tttgttgctg caacaccaat ttccggtcca cagtttaacc caattgctaa tgatgattgt
     6541 tgtgaaaggg atgaatttag tagattcaca atagataata tctttgcatt tgattcttta
     6601 gcaattttta ctgcttctaa cacatcagca ctttcaccac tttgtgagat tgtaattagt
     6661 gttgaattag gttcaatttc atttggtgaa aatggtaatt cactagaaat taatgtattt
     6721 attttaattt ttgcgaattt tgacatgaga tattttgtta tttctgcagc attgtaactt
     6781 gttccacttc cagtaatgta caagtttttt gcttgtttga tgttatctac aaactcttca
     6841 atttgattat tatccacagc acgtaatatt gtgtctggtt gctctgaaat ctcctttaat
     6901 gtataatgag caaaatctcc tttgtaaaca tcagcaaatt cttttgaaac ttttgtgact
     6961 tgatatttta ctgaagttcc atcaaaatcg aaaatatgta ttccagaacc atccacaatc
     7021 acaaaatctt cattatcaac gtaaattgca tcatctgttt tttctatgaa acccaggaca
     7081 tcactagata tgtaatgact attttttcca actccaacga tcagaggctc atgaaatctt
     7141 gctgccgcaa gtgtaccatc ttcaaaaatc acaacaaaag aataatgccc ttttaattga
     7201 ttgactgttt tcataataga ctgtttgata tcagcggttt catcaaaatt cagctgaatt
     7261 aaattagcaa ttacttctgt atcagtctca ctctgaaatt tatatccttt attctgtaac
     7321 tcagatttca attcttcaaa gttttcaata attccattat gagcaatggc aatttttcca
     7381 gaattacttg aatgtggatg ggcatttttt tccgtgactt tgccatgtgt cgcccatcgt
     7441 gtatgtccga taccaatatt tccaggaaga gtatcaagca ttatactatt gtttacctca
     7501 gcaacctttc caacaccttt ttttagtaca attttctttc ctgattctgt cgcaattccc
     7561 acactgtcgt aacccctata ctccatcttc tgtagtcctt taacaagtat aggtgcagca
     7621 gactccaatc cggaataacc aataattgaa cacattttaa ttctgaagaa attagatatt
     7681 gtctgctgat aagcttaact tacgattcgt tagttgattc tgtatcactt tcttgtttag
     7741 cagcaggttc tgtatcactt tcttgtttag cagcaggttt ttcattttca gattgttcat
     7801 tagcattatc ctccaaattt tctgtctcgg gacttcctaa atctgaactt ggttttgtta
     7861 atgtttgatt atatgatgat tcagtaatca aatgtatttt ttcctcttca tcagttttta
     7921 cagtatatga tggaattgta ataacactat ctcctatcat tacatgtcca tgaacaatcg
     7981 cttgccttgc ttgatatggt gtcttaaagt ataatttttt ctgtacaata gtttgtaaac
     8041 gacgtgataa caaatcagta acttgtagat tcaaaacatc atctaatgta gagtcattat
     8101 ttactaatcc aatttttgaa agtgagttaa ttagtatagg ttctttttca tcacgaattt
     8161 cctgtcttag agcaagtaat atacgagctt gatgtctcac tcgtgataat tcagtattag
     8221 ctttccataa ttctcgttta gttttcagtc caaatgtacc tagagttttt aattcatcca
     8281 tcataagatc ataattcaat ggacgttttg gttttttcca aactcttctt ggtgttttag
     8341 gatctcccat ttatttcgcc tatttctttt caggttttgc agattccttt ttttctacag
     8401 tacctttact ttctgttttt ggtttttcgt cttttgtatt agttactggc gtttcagcag
     8461 caccttcagc agcagcacct tcagcagcag caccttcagc agcaggagct ccagcaccac
     8521 caccaccagt tgatggcatg attttacctc ccttagcaac cccgacagct cctccttttc
     8581 tacctgtaca tctcgtacgt tgacctctaa cttttaatcc atacatatgc cgataacccc
     8641 tccaacttcc agcagttttc tctcgttcta tatcatttct gacagtaaat gcaatatcag
     8701 atgttaccag atgtttgttg acaccagtct caagatcttt tcttctattc aaaaaccaag
     8761 acggaaaatc ggatgatagt ggatccttta caattttttc aataagttga atattatcag
     8821 ttgatagatg acccattctt tgattaggat taattttcag tgtgtttagg atagtattag
     8881 caaacatgta acctacaccc cttatttgtg taagcccaac aattattttt ttatctccag
     8941 gaatgtcatt tcctaaaatc cttactatat gttcatattg cgtactcaag tatcaagatt
     9001 ttaaatatcc ccattaaaaa ccatgctgag atttttgata cccaattcga tcatattcat
     9061 agcattatta tccatctatt caagaataat aatatgtcaa atgatatttc atctaacgat
     9121 ga
//
LOCUS       DASCPJ010000062         7388 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000982926, whole genome shotgun sequence.
ACCESSION   DASCPJ010000062 DASCPJ010000000
VERSION     DASCPJ010000062.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000982926"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="QGF01_02845"
     CDS             <1..794
                     /locus_tag="QGF01_02845"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HJN19867.1"
                     /translation="NTPDGSAIFRLPEHVDRFFNSAKMYGMKMRYSKKQISNAIIKTV
                     KTSGLKECYIRPLAYYGYGTMGLTPTLNKVDVSIACWKWKMGESKAGKFSGAKCKISK
                     WIRIDSKSQPMQSKSAANYSNAALARVEALKNGYDEAIMLNNKGNVAEGSAENIFVVK
                     NGIIKTPPLDADILNGITRDSVIQLIKKEHMKLVEKNITVNELLKADEVFMTGTAAEV
                     KSVTKIDKNAIGNGDVGEITKELQKSFMNIVMGKDKRFVRWLTRL"
     gene            831..1001
                     /locus_tag="QGF01_02850"
     CDS             831..1001
                     /locus_tag="QGF01_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19868.1"
                     /translation="MEISSSSAQDVCWFCKKGRHSECMKEMPFDAKTEGPDDCTFNTK
                     MVSCKCMHDDKK"
     gene            complement(1004..1513)
                     /locus_tag="QGF01_02855"
     CDS             complement(1004..1513)
                     /locus_tag="QGF01_02855"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJN19869.1"
                     /translation="MLKIPHHVKKIFLEQQMIVVSSVDENNMPNISPRTTFTITDDVI
                     YWIELFRHKSFENFQNSDWISVASFDKEKLKGFQLKGKVTMLQDEEKKKNISLQIIDK
                     LTRLHKERILKQLGNKVPTIASFKPFIIYSSNPVELTDVPLIIGADPAISPFVGGSHM
                     KSKFGFEIS"
     gene            complement(1519..1941)
                     /locus_tag="QGF01_02860"
     CDS             complement(1519..1941)
                     /locus_tag="QGF01_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HJN19870.1"
                     /translation="MIRKILVPLDGSRNSYRGLDEAISLARQCKASIIGIYVKDMPGV
                     YVLHPIGSIGLAVGKVGKRVIAYARSKCAKKGITFNGKILGGDPPFDIVKFAHNKRNK
                     VSMIVIGARGRGTLKGIFLGSVSNYVIHKSKIPVLVVK"
     gene            complement(1938..2426)
                     /locus_tag="QGF01_02865"
     CDS             complement(1938..2426)
                     /locus_tag="QGF01_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HJN19871.1"
                     /translation="MANNKQSLSRISKAPTQFWNMPLALNDILSDNFGMDFDKFFNHF
                     HARPLFFPRTISTSCDIEDLGKELLVKVDLPGVTKKDIKLDISENSIEISAEHKDESE
                     NKRKNFLKKERTALSYYRVLPLPVKVLSVKVKAKLSDGILSITLPKVNPTKQKKKSIQ
                     VE"
     gene            complement(2795..2935)
                     /locus_tag="QGF01_02870"
     CDS             complement(2795..2935)
                     /locus_tag="QGF01_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19872.1"
                     /translation="MPKINSEVERPYLFGWIGETVEKLDNYLADDNEETEAKEETSEE
                     NL"
     gene            complement(3073..3387)
                     /locus_tag="QGF01_02875"
     CDS             complement(3073..3387)
                     /locus_tag="QGF01_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HJN19873.1"
                     /translation="MKRLDIVVRLDKVNSVTKAISKVGVGGMTVIHARGQGTADPPLI
                     GQFYSREVIVVIADDSKVDKLKSAITNAACTKLKGDGKIFVSDVVNAFDICSGVTGLD
                     AI"
     gene            complement(3398..3817)
                     /locus_tag="QGF01_02880"
     CDS             complement(3398..3817)
                     /locus_tag="QGF01_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HJN19874.1"
                     /translation="MKKILVPLDGSNNSFRGLDHAISLAKLSGASIVGAHVSYVPGNL
                     ANPRQGFINQALLKDAKRYLNTAKKRCADNNVTFTSKILAGTPSHGIVKFGQEVRNGI
                     NMIIMGTRGLSSAKESFLGSVSNHVVHKSKIPVLLVK"
     gene            complement(4251..4324)
                     /locus_tag="QGF01_02885"
     tRNA            complement(4251..4324)
                     /locus_tag="QGF01_02885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4288..4290),aa:Lys,seq:ctt)
     gene            complement(4372..4977)
                     /locus_tag="QGF01_02890"
     CDS             complement(4372..4977)
                     /locus_tag="QGF01_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HJN19875.1"
                     /translation="MTLVSKSIDIKAPVETVFTYFARPEHVSDQLKDQERVGMTVIPM
                     DIKEGMGVGTTFRIIGDFDGKRLTWDCETTEFDRNEKISAKMIKGKFKRWEITNEFKA
                     LEENLTRVTMTVDYEMPLGPLGGIIDKLKFAKSAEKGMENALYNVRGLLEGNGSIPVY
                     ITLDAYQKLLAEKKKMNNIPVSAALTTILEKYSQIPEEIKN"
     gene            5066..5587
                     /locus_tag="QGF01_02895"
     CDS             5066..5587
                     /locus_tag="QGF01_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19876.1"
                     /translation="MKMKCATIFIILLLIFPAIGLFEAQAHPLFNSSEEWIGDHRVQI
                     STLPEIPAVGEKIQVLLRVVDADFEELENFLLGIRIFYHENQIDAIQPAVYEDGHLEM
                     DYVFEESGNHIFKVDLYDVAVDGGVLTYVFNISAQNPFGYIFIGAITCGGIIAGVIFV
                     YIYASKIRSKLRR"
     gene            complement(5577..6020)
                     /locus_tag="QGF01_02900"
     CDS             complement(5577..6020)
                     /locus_tag="QGF01_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HJN19877.1"
                     /translation="MALQYLALASLVVLYSLMFIGGYISAAGLGLTCPEWPMCPNGLL
                     PDDEYFIEWGHRLIAATTGVLIVATAVGSWITKNSHWRIRTTSALAGIFVVTQITLGA
                     LVIDMKLHALLVSIHLGIGILLFAMVLLTTLFAFRLNSKSIRTIV"
     gene            complement(6022..6585)
                     /locus_tag="QGF01_02905"
     CDS             complement(6022..6585)
                     /locus_tag="QGF01_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HJN19878.1"
                     /translation="MSENDETIYRTSAGRTGKMMAICAGLVIVGGIIFFALGDYWISE
                     LSPAGEAFYGITGEDAAPVVAQTGEDIPITLKFIESSDFRTLVFNALPGEPDNNPTIN
                     AKVGDRIIFDVINAGKSFHAFGVTLDEEGFGGILAGTDVATANAPLKPGESGFSEFIP
                     GEPGTYYYICTVPGHRDMGMVGTIIVE"
     gene            complement(6595..>7388)
                     /locus_tag="QGF01_02910"
     CDS             complement(6595..>7388)
                     /locus_tag="QGF01_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HJN19879.1"
                     /translation="PAIGMMYEIIPRFSRRPIFSHSSGVFAFVLLSIVAFASWAHHMF
                     ATGMSFTEKTVFMVGTLAAVPASAMHVFNFLATMWNGRIKFATPMMWAIGGIALFFSA
                     GAGGVVNTAMPLDFITHDSYWVVGHFHLFVFGTIAFGTIGFFYYLFPYVTRRMYNEKL
                     GVVHFILSMVGASLTFFTQHLLGLYGMPRRVFDYPEIPEWIAMNQIISIGAMIILVGM
                     GIFLVNLIYSSARGKPANMNDPFGLGGKYYYPYSQLNPHHDKAGY"
BASE COUNT         2377 a         1351 c         1276 g         2384 t
ORIGIN      
        1 ataatacacc tgatggttct gcaatatttc gactgccgga acatgttgat aggtttttta
       61 attctgcaaa aatgtatggt atgaaaatgc gttactcaaa aaaacaaatc agtaacgcaa
      121 taatcaagac agtaaaaacc agtgggctta aagaatgcta catcaggcca ttagcctatt
      181 acggttatgg tacaatgggg ctcacgccta cattgaacaa ggttgatgtt tccattgcat
      241 gctggaagtg gaaaatgggc gaatcaaaag ctggtaaatt ttcaggtgca aaatgcaaaa
      301 tttcaaagtg gataagaatt gattcaaagt cacaacctat gcaatctaaa tcagcagcaa
      361 attattcaaa cgctgcttta gcacgtgttg aagcgttaaa aaatggatat gatgaagcca
      421 tcatgctaaa caataaagga aatgtggcgg aaggtagcgc agaaaatatt tttgttgtga
      481 aaaatggaat aataaaaaca ccaccactgg atgctgacat attaaatgga attacaagag
      541 atagtgtaat tcaattaata aaaaaagaac atatgaaact agttgaaaaa aatatcacag
      601 taaatgaact gttaaaggct gatgaagttt ttatgactgg taccgccgca gaagtaaaat
      661 ctgtaacaaa aattgataaa aatgctatag gaaatggtga tgtaggagaa attaccaagg
      721 agttacaaaa atcattcatg aacattgtga tgggaaaaga caaacgattt gttcgttggt
      781 tgactcgtct ctagaacagg ttttttactg aataattagt atgagataat atggaaatat
      841 cttctagttc tgcacaagat gtttgttggt tttgtaaaaa aggaaggcat tcagaatgta
      901 tgaaagagat gccatttgat gctaaaacag aaggtcctga tgattgtacg tttaacacga
      961 aaatggtttc ttgcaaatgt atgcatgatg ataaaaaatg aagttaagaa atttcaaagc
     1021 caaattttga tttcatgtgt gaaccgccaa caaacggcga aatcgctgga tctgcaccaa
     1081 taatcaaagg tacatcagtt aattcaacag gattcgaact gtaaataata aatggcttaa
     1141 agcttgctat agtaggaacc ttatttccaa gttgcttcaa aattctttcc ttgtgaagtc
     1201 ttgtaagctt gtcaattatt tgaagagaaa tatttttctt tttttcctcg tcttgtaaca
     1261 tagtaacttt acccttaagc tgaaatcctt tgagcttttc cttatcgaat gatgcaacgc
     1321 ttatccaatc actattctgg aaattttcga acgatttatg cctaaaaagc tcaatccaat
     1381 agattacatc atctgtaatt gtaaacgtag tcctgggtga aatattaggc atgttattct
     1441 cgtcaactga cgaaacaaca atcatttgtt gctctagaaa tatttttttt acatgatgag
     1501 gaattttcag catctaagtc acttcaccac taacactggt attttggatt tgtgtataac
     1561 atagtttgaa acacttccca aaaatattcc cttaagtgtg cctctacccc ttgcaccaat
     1621 cacaatcatg ctaaccttat ttcttttatt atgggcaaat tttacaatat caaatggtgg
     1681 atctccacca agtatcttac cgttaaatgt tattcctttt tttgcacatt ttgatctagc
     1741 atatgcaatt accctttttc ccactttgcc tacggccaaa cctatggaac ctatcggatg
     1801 cagaacataa actccaggca tatccttaac gtaaattcca ataatggacg ccttacattg
     1861 tctcgccaaa gatattgctt catccaaacc gcgataagaa tttctagaac catctaacgg
     1921 tacaagtatt tttctaatca ttccacctga atcgattttt tcttttgttt cgtgggattt
     1981 acctttggaa gagttatgct taaaatccca tcagaaagtt ttgccttaac ctttacagac
     2041 aagactttga cgggtagggg caaaactctg taatatgata acgctgtgcg ttccttttta
     2101 agaaaatttt ttctcttgtt ctcactttca tccttatgct ctgctgatat ctctatggag
     2161 ttttcagaaa tgtctagttt gatgtctttt tttgtgactc ctggcaggtc cactttaaca
     2221 agcagttctt taccaagatc ctcaatatca catgaagttg aaatagtacg tggaaaaaac
     2281 aatggcctcg catggaaatg attgaaaaac ttgtcaaaat ccattccaaa attatcagat
     2341 aaaatgtcat tcaacgctag cggcatattc cagaattgtg taggcgcctt agatattcgt
     2401 gaaaggctct gtttgttatt agccatagat aaaatattac atttttacat aaaaactgaa
     2461 acgtgtttta tcatttttga aaattaacaa gcaggaattt tactgatcta tcttgttgat
     2521 aaaaattggc gtggcccatt caaacaaaac atcgtatagg agattgcctc caacaacttt
     2581 caaagaaagt tatctgtttt tcatgttttg cagagccact ctgcattcag atcatttgca
     2641 tacactgaat atactttaat cgtatttaat gtagaatctt aacaatcatt tttgagaatt
     2701 aaagtaatta acttattttt tattttatct attatgaatt agtcagatta tacattaaat
     2761 cattaattgg aatttgagac ttaaaaaaag aagtttagag attctcttct gaggtctctt
     2821 cttttgcttc tgtttcttcg ttgtcatcag caagatagtt atcaagcttt tcaacggttt
     2881 caccaatcca gccaaataga tatggacgtt ctacttctga atttattttt ggcatgatat
     2941 attatccatt atttaacata tattcagcca agtcctttct catttttgaa aataatatat
     3001 ggaaattatc aaaactgcga aatagttcgc tagtgcaagt ttcaaactac gaagattttt
     3061 ttcttcttta aattatatag catccaaacc agtaacacct gagcaaatat caaaagcatt
     3121 cacaacatct gagacaaata tcttcccgtc tcctttcagt ttagtacatg cagcgtttgt
     3181 tatggcagat ttcagcttat ccactttaga atcatccgca atcacgacaa taacctctct
     3241 cgagtagaat tgacctataa gtggaggatc agccgttccc tggccacgtg catggatgac
     3301 agtcattcca cccacaccaa ctttgctgat tgctttggtt acactattca ccttgtcaag
     3361 ccttacaaca atatccaggc gtttcatgat ttctaaacta ctttaccaga agtactggaa
     3421 ttttggattt gtggacaacg tggttagaaa cactgcccaa aaatgactct tttgctgaag
     3481 ataatccacg tgttcccatg ataatcatgt ttatcccgtt tcttacttct tgtccaaact
     3541 taacaatacc atggcttgga gtaccagcta ggattttgct tgtgaatgta acattgttat
     3601 cagcacatcg tttttttgct gtgtttaggt aacgttttgc atcttttagc aatgcctgat
     3661 ttatgaatcc ctgtcgtgga ttggcaagat ttccaggtac gtaggataca tgggccccaa
     3721 ctatacttgc accagatagt tttgccaaac taattgcgtg gtctaaacca cggaaagaat
     3781 tgtttgatcc atcaagtggc actaggattt ttttcatatt tattttattc atggatataa
     3841 tatacagttt ttacataaaa acagatgaga gttttttcat gtttaagaat cagttttatt
     3901 taaaataaat agactagaag attagcgtaa taaaaaattt gatgtgattg ggaaatttca
     3961 aaaccaaatt tgataataga ataccaaatt taaataaaat aataatcatt ttaccatggc
     4021 agaagttttc aacatttccg tctacatcta taatgtctgt caaagtgaat tgtattgtag
     4081 acggaaatta ttttcttata tttgaataaa tcttacggac attctaataa tgaaagttag
     4141 aaaaagaatc tggatgaatt ataccaaaat tgcatcagga aaatttctaa aaactgagtt
     4201 tctgaaatcc gacatgtttt atgaatccaa aaagtgactc tacaggaaaa cgggtctaat
     4261 gggattcgaa cccatgacag ccggattaag agtccggtgc gctacctggc tgcgctatag
     4321 acccataaaa aaagaaaaca atactgtctt tatttaaact tgggttaagt cttagttttt
     4381 aatttcttct ggaatttgac tatatttttc aagaattgtg gtaagtgcag cagaaacagg
     4441 tatattattc atttttttct tttctgcaag gagtttttga tatgcgtcga gagtgatata
     4501 cactggtatg gagccatttc cttccaataa gcctctcacg ttataaagag cattttccat
     4561 tcctttttct gcgcttttag caaatttgag cttgtcaata ataccaccta aaggacctaa
     4621 tggcatttcg taatcaactg tcattgttac acgtgtaaga ttttcttcta gtgctttgaa
     4681 ctcgttagtt atttcccatc tcttgaattt tcctttaatc atttttgcag atatcttctc
     4741 atttctgtca aattctgtag tctcacaatc ccaagttaga cgttttccgt caaagtcacc
     4801 tatgattcta aaggtagttc ctacacccat tccttctttg atatccatag gaataaccgt
     4861 cattccaaca cgctcctgat ctttcaattg atcagaaaca tgttcaggtc gtgcaaaata
     4921 tgtgaatact gtttcaactg gtgccttgat gtctattgat ttgctaacta gggtcaacgt
     4981 tgttcactct ttttggtcag gatataaaga ttttgccaat tactagaatg gttatcttac
     5041 attatattag taaggagata aaataatgaa aatgaaatgt gctacgatct ttattatatt
     5101 attacttatt tttccggcga tcggtttgtt cgaggctcaa gctcatccac tctttaactc
     5161 atcagaagaa tggataggag accatcgcgt tcaaatttca actttgcctg aaatacctgc
     5221 agttggagaa aaaatacaag ttcttcttag agtagttgat gcagattttg aagaattaga
     5281 gaattttttg ttaggaatta gaatttttta tcatgaaaac caaattgatg ctatacaacc
     5341 tgcagtttat gaagatgggc atctcgaaat ggattatgtt tttgaagaat ctggaaatca
     5401 tattttcaaa gtggatttgt atgatgtggc agtagatgga ggagtgttaa cttatgtatt
     5461 taacataagc gcgcagaatc catttggata tattttcata ggtgcaataa cttgcggcgg
     5521 aatcatcgct ggagtgatct ttgtctacat ttatgcttca aagattagat ctaaacttag
     5581 acgatagtac gaatagattt tgaatttaat ctaaatgcaa acaaagtggt taaaagaacc
     5641 atagcaaaaa gtaatattcc aattcctaaa tgaattgaaa ccaatagggc atgtagtttc
     5701 atatcaatta ctaatgctcc taaagtgatt tgagttacaa caaatatgcc agctaatgcg
     5761 ctagtggtac gaattctcca atgagagttt ttggtgatcc atgagccaac cgcagttgct
     5821 acaatcaata ctccagttgt tgctgcaatt aatctatgtc cccattcaat gaaatattca
     5881 tcgtcaggta aaagcccatt tggacacata ggccattcag gacaggtcaa acctaagcct
     5941 gctgcagata tgtatcctcc tataaacatc aatgaataaa gaactaccaa agatgctaat
     6001 gctaagtatt gtaaagccaa actactctac tatgattgtt ccaaccatac ccatgtctct
     6061 gtgaccaggt acagtacata tgtaataata tgttcccggt tcaccaggga taaattcaga
     6121 aaatccactt tctccaggtt ttaatggagc atttgcagtt gcaacatcag ttcctgctaa
     6181 tattccacca aatccttcct catccaatgt aacaccaaat gcatggaatg attttccagc
     6241 attgataaca tcaaagatta ttctatcacc tactttggca ttaatcgttg gattattgtc
     6301 tggttcacct ggcaatgcgt taaaaaccaa tgtcctaaag tcagatgatt caatgaattt
     6361 taatgttata ggtatgtctt cgccagtttg tgccacaaca ggtgctgcat cctcaccagt
     6421 tatgccataa aatgcctcgc cagcaggtga aagctctgaa atccagtagt cacctagggc
     6481 aaagaagatt attccgccaa caataactaa accggcacat atagccatca tctttcccgt
     6541 tcgtccggct gaagtcctgt aaattgtttc gtcgttttca ctcatcttta tcacctaata
     6601 tccagccttg tcatgatgtg ggttaagttg tgagtatgga tagtaatatt ttccacctag
     6661 accaaatgga tcattcatat ttgcaggttt tcctcttgct gaactgtaaa tcaaattaac
     6721 caaaaatatg cccataccta caagaataat catggctcca atactgataa tttggttcat
     6781 tgcaatccat tcagggattt ccggataatc aaatactctt cgtggcatgc cgtaaagacc
     6841 caacaagtgt tgtgtaaaga atgttaatga tgctccaacc attgataaga taaagtggac
     6901 cacgccaagt ttttcgttat acattctgcg tgttacatat gggaatagat aatagaagaa
     6961 tccaattgtt ccaaatgcaa ttgttccaaa cacgaagaga tggaagtgac ctacaaccca
     7021 gtacgagtca tgtgtgataa agtctaatgg catagcagta ttaactactc ctcctgcacc
     7081 tgcagagaag aataatgcaa ttccgcctat agcccacatc attggtgtag caaacttgat
     7141 tctgccgttc cacatggtag ccaggaagtt gaaaacgtgc atagctgaag cgggaacagc
     7201 tgccaatgta ccaaccataa atacagtttt ttcggtaaat gacattcctg tggcaaacat
     7261 atgatgagcc caagatgcaa atgcaactat cgataaaagt acaaaagcaa agacaccgga
     7321 ggagtggctg aatatgggtc tacgtgaaaa tctaggtata atctcataca tcattccaat
     7381 tgcaggaa
//
LOCUS       DASCPJ010000063         2635 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001017967, whole genome shotgun sequence.
ACCESSION   DASCPJ010000063 DASCPJ010000000
VERSION     DASCPJ010000063.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001017967"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="QGF01_02915"
     CDS             complement(<1..308)
                     /locus_tag="QGF01_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HJN19880.1"
                     /translation="MIEKLGIGTDITDINKFKKIPYHTNKKFYKKIFLQSEIKYCIKY
                     KNPSPHFAGKFAVKESVIKAISEKISFLNIETFHSNSKPQIRLKGKIDKKYAFRASLS
                     "
     gene            complement(318..746)
                     /locus_tag="QGF01_02920"
     CDS             complement(318..746)
                     /locus_tag="QGF01_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJN19881.1"
                     /translation="MNKKSANYKFDDITIGLTKEFSIEITESMINNFAEFSGDYNELH
                     MDENYASTTKFGKRICHGMLLASFFSRLVGMSIPGKNSLYFSQSINFQSPCFIGDQIL
                     VKGEVIEKSDSTHIITIKTTIFNQDSKCLIDGIAKVIVQN"
     gene            complement(739..978)
                     /locus_tag="QGF01_02925"
     CDS             complement(739..978)
                     /locus_tag="QGF01_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HJN19882.1"
                     /translation="MTNKLYSMIAKIMDVPESDINDQSGPETIANWTSFNSYVLLYQL
                     ETEFNVKFTIDEAMDVQIVADIKRHLNNHGVNLNE"
     gene            complement(978..>2635)
                     /locus_tag="QGF01_02930"
     CDS             complement(978..>2635)
                     /locus_tag="QGF01_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HJN19883.1"
                     /translation="FTLDGLNETIKVKCSELKVGCDTFYGGYNRYNEEILNSESKLYS
                     FSPDVCFLILDTRNILGNLFYYPYNLSVDKRREFIQNKINELINLIKSFKEKSNSKLV
                     ISNFILPTYSPYGIFETKIDYGLQEMVFDLNYKLNNICRDENSIYVYDINGFVSKHGE
                     ENVFDFQQYFFGDVKISLSHIPILANDLLGYIKPTLGLNKKCIVLDLDNTLWGGIVGE
                     DGFNKIKLGPQPPGNAYIEFQKYLLALHERGIILAVNSKNNLEDAIQVIKEHPNMILR
                     EKHFACLKINWNDKVTNLKSIAQELNIGLDSMVFLDDDQVNRDFVRETLPEVLTVELP
                     KDPSLYVSTLTELNDFDTMNITEEDKQRGKMYTQQRKRVESEKNSASFEEYLKQLNIK
                     IHIKKADEFTIPRISQLTLKTNQFNLTTKRYQEEDIKTFSQDKKKIVGCAQVEDKFGD
                     NGITSVFIIKKDNEEEWIIDTFLLSCRVIGRGVEEGILDYIINEARKNNVKRIIGNFI
                     PTKKNKPSESFLSNFGFKKVNDYWIYSLEKHSKKQSHLVLLNE"
BASE COUNT          863 a          403 c          333 g         1036 t
ORIGIN      
        1 tgagataaag atgcccgaaa agcatatttt ttatcaattt ttccctttag ccttatttgt
       61 ggtttagagt tagaatgaaa agtttcaata tttaaaaatg aaattttctc agagatggct
      121 tttatgacag attcttttac agcgaatttc ccagcaaaat gtggtgatgg atttttgtat
      181 ttgatacagt atttaatttc agattgtaaa aaaatttttt tgtagaattt tttattggta
      241 tgatagggaa tttttttaaa tttattgatg tctgttatgt ccgtacctat accaagtttt
      301 tcaatcattt gtaatcacta gttttgtact ataactttag caatcccatc aattagacac
      361 ttactatctt gattgaatat tgtagttttg attgtaataa tatgtgtaga atcacttttt
      421 tcaattactt caccctttac cagaatttga tctccgataa aacatggaga ttgaaagttg
      481 atactttgag agaaataaag cgaattttta cccggaattg acattcctac cagccttgaa
      541 aaaaaagatg ccagtaacat tccatgacaa attcttttac caaattttgt tgttgaggca
      601 taattttcat ccatatgtaa ttcattatag tctcctgaaa attctgcaaa attattaatc
      661 atagattctg taatttcaat agaaaattct ttagttagtc caatagtaat atcatcaaat
      721 ttataatttg cagatttttt attcattcaa atttacaccg tgattattca aatgtctttt
      781 gatgtcagca acaatctgaa catccattgc ttcgtcgatg gtaaatttta cattaaattc
      841 agtttctaat tggtaaagta aaacatatga attaaatgat gtccaatttg caattgtttc
      901 aggtccagac tgatcattaa tatctgattc aggtacatcc ataatttttg ctatcattga
      961 atataattta ttggtcatta ttcattcaac aataccaaat gtgattgttt tttagaatgt
     1021 ttttctaaag agtatatcca ataatcattt acttttttaa acccaaaatt tgatagaaaa
     1081 gattcagatg gtttattttt tttagttgga ataaaattac caataattct ttttacatta
     1141 ttttttctgg cttcattaat tatgtaatca agtattccct cttctacgcc tctaccaatt
     1201 actctacaac ttaaaagaaa agtatcaata atccattctt cttcattatc ttttttgata
     1261 atgaatacag atgtaatacc gttatctcca aatttatctt ctacctgtgc acaaccaaca
     1321 atttttttct tatcctgaga gaaagttttg atatcctctt cttgataacg ttttgttgtg
     1381 agattaaatt ggttggtttt taaagtgagt tgagatattc taggaattgt gaactcgtca
     1441 gcttttttaa tatgaatttt aatatttagc tgtttaagat attcttcaaa actggctgaa
     1501 tttttttcgg attctactct ctttcgttgc tgtgtataca tttttccacg ctgcttatcc
     1561 tcttctgtaa tattcatagt atcaaaatca tttaattctg ttaatgttga aacatatagt
     1621 gaaggatctt ttggtaattc aacagtaagt acctcgggaa gagtttcacg tacaaaatct
     1681 ctattaactt gatcatcgtc cagaaatacc atactatcta atcctatatt gagttcttga
     1741 gcaatgctct tcaaattagt aaccttgtcg ttccaattaa tcttcaagca agcaaaatgt
     1801 ttttccctca gaatcatatt tggatgctct ttgattactt gaattgcatc ctctagatta
     1861 tttttgctat ttacagctaa aattattcca cgttcatgaa gagcaagtaa atacttttga
     1921 aattctatgt aagcgtttcc tgggggttgt ggtcctaatt taattttatt aaatccatct
     1981 tcacccacta taccacccca caaagtatta tcaagatcta aaacaataca cttcttattg
     2041 agacctaaag ttggtttaat atatcctaga agatcatttg ccaaaattgg aatgtgactt
     2101 aatgatattt ttacatcacc aaaaaaatat tgttgaaaat caaatacatt ctcttcacca
     2161 tgttttgaaa caaatccatt aatatcataa acataaattg aattttcatc tctacaaata
     2221 ttatttagtt tgtaatttag atcaaaaacc atttcctgta aaccgtaatc tattttggtc
     2281 tcaaatattc catatggtga atatgttgga agaataaaat tagaaatgac taattttgaa
     2341 tttgattttt ctttaaaaga ttttattaga ttaattaatt cgttgatttt attttgtata
     2401 aattctctac gtttgtctac tgataaatta tatggataat aaaataaatt ccccaaaata
     2461 tttctagtat ctaaaataag aaaacaaaca tctggagaaa aagaataaag tttgctttca
     2521 ctatttagta tttcttcatt atagcgatta taaccaccat aaaatgtatc acatccaact
     2581 tttaattctg aacattttac cttgattgtc tcattcaaac cgtctaatgt gaaac
//
LOCUS       DASCPJ010000064         2024 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001027554, whole genome shotgun sequence.
ACCESSION   DASCPJ010000064 DASCPJ010000000
VERSION     DASCPJ010000064.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001027554"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            93..1346
                     /gene="hisD"
                     /locus_tag="QGF01_02935"
     CDS             93..1346
                     /gene="hisD"
                     /locus_tag="QGF01_02935"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HJN19884.1"
                     /translation="MIKILQVRNIDSFVESRRQKTSDKDIHTVLAILDDVRKNGDSAV
                     KKYEQKFNGRKTSQLRVSEKEINKEKITEAQFDALRSSALRVSITQRRLKKGLIGSVS
                     KLRGISFIPIASVGCYVPGGQARYPSSAIMSAITAAEAGVKRIVITSPPGPDGKIDPM
                     TITVAKKCGAEIYKVGGAQAIGALAYGTKSIPKVDKIVGPGGKFVSIAKLIVSEQTSI
                     DMIAGPTELGIIADSSANPELVALDLISQAEHSNDTMCFVITTSKTVAKQIQKLLEKL
                     ISDVERSVIVKQSISKNGFIAVCKNEKEMVELANKIAPEHMELMVKNAKTLSKKITGA
                     GLLLIGKNTPSSASDYLLGTNHILPTNGFGRTRGGLSVLDFLKLQTTVEEKKSGLKSI
                     SDKLKTLTDAEGLPNHYEAVKRRLD"
     gene            1343..>2024
                     /locus_tag="QGF01_02940"
     CDS             1343..>2024
                     /locus_tag="QGF01_02940"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HJN19885.1"
                     /translation="MNKEFQKKLNELSKLTGYEKPKKISGSLKLDSNENFVIGKQFQS
                     DLIDAAKKRCDVREYPLGGTEKLISKLSKYLKVPSNMVGIGNGSDQILDLFLANFCTR
                     KTKILTSDPTFAFFEERCKLYGVKMIKIPFSDGMTLNVDQFLSKSKNADLIYIDSPNN
                     PTGFQFSKNDIQKIIKKFKGPIIIDEAYGEFSDYSVVNLVKNNDNLIVIRTFSKAFGL
                     AGLRLGYFV"
BASE COUNT          740 a          331 c          415 g          538 t
ORIGIN      
        1 gttcctaaac taagaaagat tgcacaggga ttggtagttc acgagccaag acaaatactg
       61 gaacttgagg agataaaacg tgacgaagag aattgatcaa gatattacag gttcgtaata
      121 ttgacagctt tgtagaatct aggcgtcaaa agacctcaga caaagacatt cacacagttc
      181 tggcaatatt ggatgacgta agaaaaaatg gagattctgc tgtcaaaaaa tacgaacaga
      241 aatttaatgg aagaaagact tcacaactac gtgtaagcga aaaagagatc aataaagaaa
      301 aaattactga agcacagttt gatgcactac gaagttctgc tttaagagta tcaattactc
      361 aaagacgact taagaaagga ttaattggct cagttagcaa gcttcgaggc atctcgttta
      421 taccgattgc aagtgtaggt tgttacgtac ctggaggcca agctaggtat ccaagttctg
      481 ccataatgtc tgctatcact gcagctgaag ctggtgtcaa gagaattgta attacatccc
      541 caccaggacc tgatggaaag attgatccaa tgacaattac tgttgctaag aaatgtggtg
      601 cagagattta caaggttggt ggggcacaag caattggtgc attggcatac ggaacaaaat
      661 caatacctaa agttgataaa attgttggac ctggaggaaa atttgttagc attgcaaaat
      721 taattgtcag tgaacaaact tctattgata tgattgcagg accaacagaa cttggaatca
      781 tagcagattc aagtgcaaat cctgagttag ttgcattaga cttgatttct caagcagaac
      841 acagcaatga tactatgtgt tttgttatca caacgtcaaa aaccgtggca aaacaaattc
      901 aaaaattact tgaaaaatta atttcagatg tagagaggag tgtaattgta aagcaaagta
      961 tctcaaaaaa tggattcatt gctgtatgca agaatgaaaa agaaatggtt gaattagcaa
     1021 acaagattgc accagaacac atggaactga tggtgaaaaa tgccaaaact ctttcaaaaa
     1081 agattacagg cgcaggctta ttgctaatag gaaaaaacac accatcttct gcaagtgatt
     1141 accttctggg tactaaccac atacttccaa ctaatggatt tgggagaacg agaggcggat
     1201 tgtctgtttt agattttcta aagctacaga ctacggttga agaaaaaaaa tcaggattaa
     1261 aatcgatctc agacaaacta aaaacattaa cagatgcgga aggcttacca aaccactacg
     1321 aggcagtaaa gaggagatta gattgaacaa agaattccaa aagaaactaa acgagttgtc
     1381 aaagctcact ggatatgaaa aaccaaagaa gatttcgggt tcattaaagc tggattccaa
     1441 cgagaatttt gtcattggga agcagtttca gtcagatcta attgatgctg caaaaaagcg
     1501 ctgtgacgtt cgagagtatc cgttgggagg aacggaaaag ctaatctcga agttatccaa
     1561 atacctcaaa gtaccatcaa atatggttgg tatcggaaac ggttctgatc agatacttga
     1621 cttgtttctg gctaatttct gtacaagaaa gaccaaaatt ttgacatccg atcctacttt
     1681 tgcatttttt gaagagagat gcaagcttta cggagtcaaa atgatcaaga ttccattttc
     1741 tgatggtatg acattaaacg ttgatcaatt tttgtcgaaa tcaaagaatg cagatttgat
     1801 ctatattgac tcaccaaaca accctacagg ctttcaattt tcaaaaaatg acatacaaaa
     1861 aattatcaaa aaattcaaag gtcctatcat catagatgaa gcctatggag aattttcaga
     1921 ctattcggta gtaaatctgg taaaaaacaa tgacaatcta attgttatca gaacgttctc
     1981 aaaggcattt ggattagcgg gtttgagatt gggttatttt gttg
//
LOCUS       DASCPJ010000065         3701 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001047350, whole genome shotgun sequence.
ACCESSION   DASCPJ010000065 DASCPJ010000000
VERSION     DASCPJ010000065.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001047350"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(101..613)
                     /locus_tag="QGF01_02945"
     CDS             complement(101..613)
                     /locus_tag="QGF01_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19886.1"
                     /translation="MTKDPKFNSEDEKPKKESDLIKFFKKRSFIYLLCAVMFVVFFVP
                     EMMQPSELDQKITEILDSDDENHAWSILKSYKGTDDKGSTLGEIIMIQIENAYPSEKI
                     LKHHDTSIEISALDAQEEQGSGFYQVHFSFKTYDNAKDYLWYVNIQTDEIIPLNDGAR
                     KMMNIINNYD"
     gene            complement(681..812)
                     /locus_tag="QGF01_02950"
     CDS             complement(681..812)
                     /locus_tag="QGF01_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19887.1"
                     /translation="MMNTPPKSGETQGPLCRICEKPFSEHTFQQQQECAKKMQDKGQ"
     gene            900..1058
                     /locus_tag="QGF01_02955"
     CDS             900..1058
                     /locus_tag="QGF01_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HJN19888.1"
                     /translation="MAIATCRDYGYDCDFVVEGDIDKVAIEFGKHSAEQHGIEYSKET
                     LTKFMVKK"
     gene            complement(1071..1976)
                     /locus_tag="QGF01_02960"
     CDS             complement(1071..1976)
                     /locus_tag="QGF01_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HJN19889.1"
                     /translation="MLSSWLRAIRIRFLLASIIAVSTGLAISWWTGNSITIFDAVLTF
                     LGVLALHASVDLLNDYWDFKRGIDSETVKTKLSGGSGVLPEGLLDTKHVYRAGIIFLI
                     IGALIGTYFVITDGIIIGVILAFAILSIYFYSTKIVNWGLAEVFVAIKGTLIVIGTCY
                     IQTSQINESMILGGIVVGVLSSFVLFITSFPDYDIDKAKGRKTLVISLGLRRSCTVFW
                     LFPIIVYTLTIVSVILDVFPLICLCILLTFPLIIKSGKKLKTSYSKLSGLIPAMSSTL
                     VFSRITGAIFFICFIIDALVMRNVF"
     gene            complement(1976..2377)
                     /locus_tag="QGF01_02965"
     CDS             complement(1976..2377)
                     /locus_tag="QGF01_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HJN19890.1"
                     /translation="MDNYFTIKSANEMLPTIIEKFQKIKQEKNEVEKMEQKLQMNLSG
                     TSNLDDYVTLKQNLNASVTRFYISIEELEKTGVVLKGLEEGLLDFPSKKFDDEIWLCW
                     KEGETEIKFWHEKDVGFNGRKPIDVNKESLV"
     gene            2498..2926
                     /locus_tag="QGF01_02970"
     CDS             2498..2926
                     /locus_tag="QGF01_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19891.1"
                     /translation="MGLFGRKRAICTICNKEITHKHKAKREWYVKSPLCGDCYLDKMK
                     QSYDATITQSCVVCKIKSKVTDLWEPRWQWDMEGLLCKKCFDEKESNFETEKDFCSIC
                     GCKLKAFNRFHPKGKWKLKGLLCKNCWDSQKIHEKENSSK"
     gene            complement(2919..3131)
                     /locus_tag="QGF01_02975"
     CDS             complement(2919..3131)
                     /locus_tag="QGF01_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rbp10"
                     /protein_id="HJN19892.1"
                     /translation="MSDETTPEADNEVTTPEVNEEKFEVIYTCLRCGTPVSNIELSRL
                     PEIKCICGFRVFSKLRPPIVKTVKAI"
     gene            complement(3167..3475)
                     /gene="rpsJ"
                     /locus_tag="QGF01_02980"
     CDS             complement(3167..3475)
                     /gene="rpsJ"
                     /locus_tag="QGF01_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HJN19893.1"
                     /translation="MVQPARVKLTSTNLIKLGDVCNEILEIGKKSGVKVKGPTPLPVK
                     RLNISTRKSPCGSGTETYEKWEMKMHRRIIDINADDKAIRQLMRLKIPDYVYIELSLT
                     "
     gene            complement(3487..>3701)
                     /locus_tag="QGF01_02985"
     CDS             complement(3487..>3701)
                     /locus_tag="QGF01_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 1-alpha"
                     /protein_id="HJN19894.1"
                     /translation="KIDPATGGTAEENPKFLKVGDSAIVRIKPVRPTCIETFKEFPEM
                     GRFALRDMGATIAAGVVKDITEEHKP"
BASE COUNT         1262 a          632 c          526 g         1281 t
ORIGIN      
        1 cccaaaaaaa attttttctg tattgaatga ttaggttttg gtttgtatgg aaatgccata
       61 ttatgaatat gatttcttct taataaaatt aaatggtcga ttaatcataa ttattgatga
      121 tgttcatcat ctttcttgca ccatcattta gcggaattat ttcatcagtt tgtatattta
      181 cataccacaa atagtcctta gcattatcat atgttttaaa agagaaatga acttgataaa
      241 aaccagaccc ctgttcttcc tgcgcatcca atgctgatat ttctatagaa gtatcatgat
      301 gcttgagtat tttttcacta ggatacgcat tttctatctg tatcattata atttcaccaa
      361 gtgttgatcc cttatcatca gtacccttgt atgatttcaa aatactccat gcatgatttt
      421 catcatcact atccaaaatt tcagttatct tttgatctaa ttcacttggt tgcatcattt
      481 ctggaacaaa aaatactaca aacatgactg cacataataa ataaataaat gaccttttct
      541 taaaaaattt tattaaatct gattctttct ttggtttctc atcttcacta ttgaattttg
      601 ggtccttggt cattctgtat attaccacta catgaaaaga gaataaaaaa cctaacttta
      661 cctattaatt gatatatatc ctattgaccc ttatcttgca tttttttagc acattcttgt
      721 tgttgctgaa aagtatgctc agaaaatggt ttttcacata ttctacataa agggccttgt
      781 gtttctccac tttttggtgg ggtattcatc atttttgatc atttaactga caatattata
      841 caaaataaat atgctcatta gttcagttag tttattaatt ctaccatgcc cgatgaatta
      901 tggccattgc aacttgccgt gactacggat atgactgcga ctttgtagtt gaaggtgata
      961 ttgataaagt tgctattgaa tttggtaagc attcagctga acagcatggg attgaatatt
     1021 caaaagagac actaacaaaa tttatggtta aaaaataact aataaactga ttaaaagaca
     1081 ttcctcataa ctaacgcatc tataataaaa catataaaaa atattgcacc cgtaattctg
     1141 ctaaatacca atgttgaact catggcgggt ataagaccgg ataatttact gtatgatgtt
     1201 tttaattttt ttccagattt tataattaat gggaatgtta gtaaaataca taggcaaata
     1261 agtggaaata catccagaat tacagaaact atagtaagag tataaacaat aattgggaat
     1321 aaccaaaaaa ctgtacaaga tcttctcagt cccaaactta tcacaagtgt ttttctacct
     1381 tttgctttat caatatcata atcagggaat gatgtaataa atagaacaaa tgatgataat
     1441 acaccaacaa caattccgcc taaaatcatt gattcattta tttgagaagt ttggatgtag
     1501 caagttccaa tcacaattaa ggttcctttt attgcaacaa aaacttctgc taatccccaa
     1561 ttaacaattt tagttgaata aaaataaatt gaaagtatag caaatgcaag aattacccca
     1621 ataattatac catctgttat aacaaaatat gttccaatca gtgctccgat tattaaaaat
     1681 ataattccag cacggtaaac atgtttagta tctaacaatc cttcaggtaa aacaccagat
     1741 cctccactca attttgtttt cacagtttca ctatcaatcc cacgtttgaa gtcccaataa
     1801 tcattcagta aatcaacact tgcatgaaga gcaagtacac ccaaaaatgt caatacagca
     1861 tcaaaaattg ttatagaatt accagtccac caactaattg ctaatccggt agaaacagct
     1921 ataatcgatg ctaataaaaa acgaattcgt atggcacgta accaggaaga aagcatcaca
     1981 ctaatgattc tttattgaca tcaattggtt tacggccatt aaaaccaaca tctttttcat
     2041 gccaaaattt tatctcagtt tcaccttcct tccaacataa ccaaatttca tcatcaaact
     2101 tttttgaagg aaagtcaagt agaccttctt caagtccttt taatacaacg ccagtttttt
     2161 ccaattcttc tatactaata taaaatcttg ttacagatgc attaagattt tgtttaagcg
     2221 ttacataatc atccaaatta gatgttcccg ataaattcat ctgtaatttt tgttccattt
     2281 tttcgacctc atttttttct tgctttattt tttggaattt ttcaatgatt gttggtagca
     2341 tttcatttgc agatttaatc gtaaaataat tgtccataaa ttgttcagaa ttaagagaaa
     2401 tttaaaggat atagaagcaa atacgcatat gatgcattag aactcattct catgtaaata
     2461 ggaaagatat aatattttaa agaaaatatt tgtaatcatg ggtttgtttg gacgtaaacg
     2521 tgcaatttgt actatatgta ataaggaaat tacacataag cataaagcaa aaagagaatg
     2581 gtatgttaaa tcaccattat gcggtgactg ttatttagat aaaatgaagc aatcgtatga
     2641 tgcaacgatc actcaatcat gtgttgtatg taaaataaaa agtaaggtca ctgatttatg
     2701 ggaaccgcga tggcagtggg acatggaagg tcttttatgt aaaaaatgtt ttgacgaaaa
     2761 agaatctaat tttgagactg aaaaagattt ttgtagtatt tgtggatgta aattaaaagc
     2821 attcaataga ttccatccaa aaggcaaatg gaagttaaag ggtcttttat gtaaaaactg
     2881 ctgggactcc caaaaaatac acgagaagga aaatagttct aaatagcttt tactgttttg
     2941 actataggtg ggcgtaattt agagaatact ctaaagccac aaatacattt tatttctggt
     3001 aatctgctta gttcaatatt agataccggt gtaccacatc tcaaacaagt atatatcact
     3061 tcaaattttt cctcattaac ctctggcgta gtaacttcgt tgtcagcttc aggggttgtt
     3121 tcgtcagaca tgattttata aaaaaaagac cagaataaaa tcctaactat gtaagtgata
     3181 attcaatgta aacataatca ggaattttca atcgcattaa ttgtcgtata gccttatcat
     3241 cagcattgat gtctattatt cttctatgca ttttcatctc ccatttttca tatgtctctg
     3301 ttccactacc acagggagat tttcttgtac taatattcaa tcgtttcaca ggaagcggtg
     3361 ttggaccctt aacttttaca ccactttttt tacctatttc taaaatttca ttacaaacat
     3421 cacccaattt aatcaagttt gtactagtta atttcacacg agctggctga accattctaa
     3481 aactcgctat ggtttgtgct cttctgtaat atctttaacg acaccagcag ctattgtagc
     3541 acccatatct cgtaatgcaa atcttcccat ttcaggaaat tctttgaatg tttcaataca
     3601 tgttggtcta actggtttaa ttctaacaat agcagaatca ccaactttaa ggaatttagg
     3661 attttcttct gctgttccac cagtagcagg atctatttta g
//
LOCUS       DASCPJ010000066         2977 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001049327, whole genome shotgun sequence.
ACCESSION   DASCPJ010000066 DASCPJ010000000
VERSION     DASCPJ010000066.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001049327"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /gene="metG"
                     /locus_tag="QGF01_02990"
     CDS             <1..1408
                     /gene="metG"
                     /locus_tag="QGF01_02990"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HJN19895.1"
                     /translation="AAFNIEFDFFYRTSSPENKEFVQYVFKKLENAGHIYENDIIQFY
                     CNNDKKFLPDRYVIGTCPYCKSEEQYSDLCEKCGRVPEEIENPHCSICGQPPVKEKTS
                     HYFFKLKNFSEKLSQYLEENQNLQKDVKKYVQNWIKEGLTDWDITRDISWGIPVPIDG
                     VNDKVFYGWFDNHLAYISSTIKFLNDNGKDGKKFWNESDIYHFIGKDIVYHHYLFLPA
                     MRLGIEEEYKLPDYIPTRGHLTLQSKKISKSRNWYIGLKDFLQIFPADYLRFYLISIN
                     PYSQDDLNFDWNEFATKINSELIGNLGNLVNRVLGFTVKSFGGVIPEPEEFDDADKES
                     EKLIKEFVTDIGTLMEQNHLDRALKKIFQFSAHFNQYFQHKEPWKNNPGTNSCIYLSV
                     NAVRSLAIATYPFLPESSQKIWVQLGMHDNISAQGFDEISNISIKQGHKLGKISPLFQ
                     KVEESVIKEQSAKLGDSM"
     gene            1410..2162
                     /locus_tag="QGF01_02995"
     CDS             1410..2162
                     /locus_tag="QGF01_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HJN19896.1"
                     /translation="MKKIFPRISTRGFYDLDSGTTLQNESYNVYPKRMFDALVGTKEI
                     TIMIHGLRNNAAGALTKFITARRRLAALGYKHPVIGYSYDSNTVGAQYISYALHALHI
                     GVIIANKNGRNLAKFVEDFKHKSPETKIRLVGHSLGAHVIMSTIKNLARNTKNKGIIE
                     AVYFFGGSIPSNSLNMKNGSIAQKIVARKIRNYYSPYDDVLRLADYWNWVDKPIGYRG
                     SDGKTIPKYSQTMVKPKNHRFVSYASVLRAFP"
     gene            2159..>2977
                     /locus_tag="QGF01_03000"
     CDS             2159..>2977
                     /locus_tag="QGF01_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HJN19897.1"
                     /translation="MRSVNFVVLGEQTIANDFGKKGTVTDLTVYDRKESDIIRTWVVP
                     NGFPEKIQPLLQAINIAEYVIFYVASLDRYTGEQIIALDMLNKKNGILSHSYEVDENR
                     LHSMIKGTVLQNYKKIDSLNIKEELVKLPPVSNDGPAQIVIDHSFDVKGVGTVVLGKV
                     LQGTINQYDNLKHLPSGTDVMVKSIQMHDDDVKQAVCPARVGLSLKGIKPDEVGRGDV
                     ITSDESFEIKNELEIDFKQSPFYKGEISENQMCLVSIGLQVKAAKFSSISPVKLI"
BASE COUNT         1086 a          425 c          554 g          912 t
ORIGIN      
        1 tgcagcattt aatatagaat ttgatttctt ttatagaaca agttctcctg aaaacaagga
       61 gtttgttcaa tacgtattca agaaacttga aaacgccgga catatttatg aaaatgacat
      121 aatacagttt tactgtaata atgataagaa atttttacct gatagatatg taattggtac
      181 atgtccatac tgtaagtcag aagaacaata ttctgattta tgtgaaaaat gtggtcgtgt
      241 tccggaagaa attgaaaatc cacattgttc aatttgtggg caaccgccag ttaaggaaaa
      301 aacatctcat tattttttca aactgaaaaa tttttcagaa aaactgtcac agtatcttga
      361 ggagaatcaa aacctacaaa aagatgtcaa aaaatatgta cagaattgga tcaaggaagg
      421 actaactgat tgggatatca ctcgtgatat atcttgggga attcccgttc cgattgatgg
      481 cgtgaatgat aaggtgtttt atggttggtt tgataaccat cttgcataca tttcatctac
      541 aataaaattt cttaatgata atggtaagga tggcaaaaaa ttttggaacg aatctgacat
      601 ttatcatttt attggaaagg atatagttta tcatcactat ctatttttgc cagcaatgcg
      661 tttggggatt gaggaggaat acaaacttcc tgattacatt ccaacaagag gccatcttac
      721 attacagtca aaaaaaatat caaaaagcag aaattggtac ataggtttga aagatttctt
      781 acaaatattc ccagctgatt atctcagatt ttatcttata tccataaatc catactctca
      841 ggatgatctc aattttgatt ggaatgagtt tgcaactaag ataaactctg aattgatagg
      901 taatcttgga aatttagtga atcgtgtact aggatttact gtaaaatcat ttggtggtgt
      961 tattccagaa ccagaagaat ttgatgatgc ggataaagaa tcagagaaat taatcaaaga
     1021 atttgttacg gatattggta cattaatgga acaaaaccat cttgatagag cattaaaaaa
     1081 aatatttcaa ttttcagctc attttaacca atatttccag cataaagaac catggaagaa
     1141 taatcccggt acaaattcat gcatatatct atccgttaac gctgttcgaa gtttggcaat
     1201 agctacatat ccatttttac cagaatcatc acaaaaaatt tgggtgcagt tgggtatgca
     1261 tgataatatt tcggcacaag gttttgatga aatatcaaac atatcgataa agcagggtca
     1321 caagcttggt aaaatatctc cactctttca aaaagtagaa gaatctgtga taaaggagca
     1381 aagtgctaag cttggagata gcatgtaaat tgaaaaagat ttttccacgt atatctacaa
     1441 ggggttttta tgatttagat tctggtacca cattacaaaa cgaatcatat aatgtgtatc
     1501 caaaacgaat gtttgatgca ttagttggaa caaaagaaat aaccatcatg attcatggtt
     1561 tacgaaataa tgcagctggt gcacttacaa aatttattac agcaaggaga aggttagctg
     1621 ctcttggcta caaacatcct gttataggat atagttatga ttcaaacacc gttggtgcac
     1681 agtatatttc atacgcactt catgctttgc atataggtgt gataatagcc aacaaaaatg
     1741 gaagaaattt ggctaaattt gttgaggatt ttaaacataa aagtccagaa acaaaaatca
     1801 gacttgtggg acattccctt ggggcacatg taattatgag tacgataaaa aatctcgcaa
     1861 gaaatacaaa aaataaagga attatagaag cagtttattt ttttggcggt tctataccta
     1921 gtaattcatt aaatatgaaa aatggttcta tagctcaaaa aattgtcgca agaaaaatta
     1981 gaaattatta tagtccatat gatgatgttt tacgtttagc tgattattgg aattgggttg
     2041 ataaaccaat tgggtatagg ggatctgatg ggaaaacaat accaaaatac tctcaaacta
     2101 tggtaaaacc aaaaaatcat cgatttgtta gttatgcatc tgtacttagg gcatttccat
     2161 gagatcagtg aattttgttg ttcttggtga acaaacaata gcaaatgatt ttggaaaaaa
     2221 aggcacggtg acggatctta cagtttatga tagaaaagaa tcagacataa ttaggacatg
     2281 ggtagtacct aatggatttc cagaaaaaat acaaccatta ctacaggcga taaatattgc
     2341 agagtatgtt attttttatg ttgcatcgct tgataggtat actggagaac aaatcattgc
     2401 actcgatatg ctaaataaaa aaaatggaat actaagtcat tcctatgagg ttgatgaaaa
     2461 tcgtcttcat agtatgataa aaggaactgt attacagaac tacaagaaga tagattcact
     2521 aaacatcaag gaagaattag taaaacttcc acctgtttca aatgatggtc ctgcacaaat
     2581 tgtgattgat cattcttttg atgtgaaagg tgttggtaca gtggttttgg gtaaagtgtt
     2641 acaaggaaca ataaatcaat atgataatct taaacatctt ccatcgggta ctgacgtgat
     2701 ggtgaagtca atccaaatgc atgatgatga cgtgaagcag gcagtttgcc cagcaagggt
     2761 tggcctatca ttaaagggaa tcaaaccaga cgaagttgga agaggagatg taattacttc
     2821 tgatgaatca ttcgaaataa aaaatgaact tgaaattgat tttaaacaat caccattcta
     2881 taaaggtgag atttctgaaa atcaaatgtg tcttgtaagt attggattac aggtaaaagc
     2941 tgcaaaattt tcctcaatat ctccggtcaa attaata
//
LOCUS       DASCPJ010000067         7337 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001060308, whole genome shotgun sequence.
ACCESSION   DASCPJ010000067 DASCPJ010000000
VERSION     DASCPJ010000067.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001060308"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="QGF01_03005"
     CDS             complement(<1..96)
                     /locus_tag="QGF01_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJN19898.1"
                     /translation="MDKLDTEILNEIQWEFPLVIKPFDEIAKKFGI"
     gene            187..840
                     /locus_tag="QGF01_03010"
     CDS             187..840
                     /locus_tag="QGF01_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HJN19899.1"
                     /translation="MIIDLHLKGNLVIVIGSGNEGVKKVNSLLTQDCKILVISDKLNS
                     QIERYVKQGKIKFKKTKLKDATFLSKYKPYLVMATTSDRMLNRKIVEKARKMKSYAYA
                     SDDPEISDFAHPSIINIADTIQIAISTGGSSPAMARKIKLKAEPFFKKNISNEDIYQI
                     KLQKFARFEAKQVLSTQLDRKKFLYAVMNDKRVKGLLKEGKYKMSQVQVKKMLKEWS"
     gene            837..2093
                     /gene="hemA"
                     /locus_tag="QGF01_03015"
     CDS             837..2093
                     /gene="hemA"
                     /locus_tag="QGF01_03015"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HJN19900.1"
                     /translation="MKQNIINARITFRKSPIHVLEKFAFKDLSDAYLSFKKHASISEC
                     VIIQTCNRVELFTASNNHDVHKIKKTWASLTGLEENAFADTLEICDNHDAFEHLLKLT
                     SGLESLVVGEEQILGQIKESITVARNTKASGEKLNKLFDTSIRIGTRIRNSTGISKGG
                     ISLGSMAVKLAEENVDDIKSKNVLLIGTGEAATMVAKSLNKRGYEFHVTSRTVERSTG
                     FSQTLGGKPIKFEDVLSTFDNYEIIFVATIAPYFLITFDKMIESMKNKKSGMMILDLS
                     NPRTVDEQVATIPGIKLMNIDQIAEMVDKNMTKRKEKVSTVQNIISEEVPIIEATMKR
                     LDAEPLVKDVFTNADYMRIKELQKALQMLGETDEKRIKIIDELTKAVVESIVSAPMNN
                     LRKASEQGNPDLLETASKLFNYKKKD"
     gene            2138..3124
                     /gene="hemB"
                     /locus_tag="QGF01_03020"
     CDS             2138..3124
                     /gene="hemB"
                     /locus_tag="QGF01_03020"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HJN19901.1"
                     /translation="MATFPSKRLRRLRVNENIRSLVQEVRLSVNDLVCPIFVEEGLKE
                     KKSVGSMPDIVRLPLSEISNEVQNISDLKIPAVMLFGIPSQKDESGTSAYAENGIVQR
                     TISEIRSNFGDKISIMADVCLCQYTSSGHCGIINGKKIDNDLSIKTLTDIAVSQAKMG
                     VDVVSPSAMMDGQVSAIRKGLDDANFKDVIIMSHSAKHSSSFYSPFRDAAECAPQFGD
                     RKTYQVPFTNPREAMREVEADIEEGVDIVMIKPALSYLDLIAEAKKKFNVPVSAYSVS
                     GEYALVKAAANQGWINENEVTNEILSSIKRAGADFIVTYFAKLGAKLINENS"
     gene            3121..3432
                     /locus_tag="QGF01_03025"
     CDS             3121..3432
                     /locus_tag="QGF01_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19902.1"
                     /translation="MIDDERFEIQVAFDLLPHVVGCSWATIWFRLNKIKNPTREEFRQ
                     KVTEYFKMLDPLVNIYPEDKNFEEIINYIRKRNVVEIKKISAGTNPEVERRYDRYVDY
                     G"
     gene            complement(3440..4060)
                     /locus_tag="QGF01_03030"
     CDS             complement(3440..4060)
                     /locus_tag="QGF01_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19903.1"
                     /translation="MADIIEVIAEYSYLGILFLLMAVNAAPILMPPTWIILSSFFVLD
                     TSLDPFFLALVGATGATIGRFFLKWMSGFFRRFVGKQQKSNLDVIGNFLNRKKFGYTL
                     TSFLFAVTPLPSNMLFVAYGMMRAKSIGLYIGFWFGRLLSYYVMITISAAVLVPFLQL
                     FEDRIIGIIVADVVGIGSVIFFTCIDWQALLLQRKLKFVRPKLWRI"
     gene            complement(4305..4405)
                     /locus_tag="QGF01_03035"
     tRNA            complement(join(4305..4349,4366..4405))
                     /locus_tag="QGF01_03035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4367..4369),aa:Leu,seq:caa)
     gene            4466..4759
                     /locus_tag="QGF01_03040"
     CDS             4466..4759
                     /locus_tag="QGF01_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HJN19904.1"
                     /translation="MTIEDAKNQRSDITLQGTIEEKGEPRTVNTKYGETQVCDSYLKD
                     DTGRIKLTLWGEDIEKVKNGDTVSIQGAYTTTFRNEVQLNVPKKNGKLEVISN"
     gene            complement(4788..5708)
                     /locus_tag="QGF01_03045"
     CDS             complement(4788..5708)
                     /locus_tag="QGF01_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19905.1"
                     /translation="MVSLTKKLTVLKQRKSRAKKNRTKTSETLRLAKLLYKKSVSGIV
                     SIQHKMDNFNSELEGVSTVLQQKFAQRDSITSLIEQAEDRLSSEKEKYDEIKEEVSFA
                     SGEAKKQLQFTINTVSTEIEDLHSQLKSRKKTLEKVIQSIQEYDIKKSKLNKQIKRTL
                     ESKPQLMKAIQQSKKTVAKLEKKLPSLIQAEENVHKNFLKINSIIKEQTSKKKESQAN
                     IRKKKARDAKRKSVEDKRILSMARKLANKILAEKKKASKRKASKKKAPKRKASKKKAP
                     KRKASKKKAPKRKASKKKAPKRKASKKNKK"
     gene            complement(5790..6245)
                     /gene="msrA"
                     /locus_tag="QGF01_03050"
     CDS             complement(5790..6245)
                     /gene="msrA"
                     /locus_tag="QGF01_03050"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HJN19906.1"
                     /translation="MKATFGAGCFWHVEEIFRKTKGVKSTQVGYSGGMKQNPTYEDVC
                     TDTTGHAESVEIDYDPEITSYNDLLKIFWENHNPTTLNRQGPDVGSQYRSIIFYHSEE
                     QKKLATEMKDQLNSVVKEKFKKEIVTWIEPVSNFYRAEEYHQRYLTKFS"
     gene            6282..6599
                     /locus_tag="QGF01_03055"
     CDS             6282..6599
                     /locus_tag="QGF01_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19907.1"
                     /translation="MEKADIESIPVQKTFDLKDEKDAYDAAEEMVRIGFYKEKKGFKV
                     LMAKEPKKTAKRIGYIVTTTVTASLRNDDQYRDIRYWTYHHDKERYGIVLVNSKVIDE
                     LGF"
     gene            complement(6588..7241)
                     /locus_tag="QGF01_03060"
     CDS             complement(6588..7241)
                     /locus_tag="QGF01_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HJN19908.1"
                     /translation="MQKFIFEMRSIGFFFLVISFLIITALVYAEITEQFDISVITYFQ
                     SISGNSTLDTSMWAITEIGGIIPIMVFCFVMFVWRRTRRMGLVMLLAVIIATVASGYF
                     KDYIVERPRPDLEYIGTELPLEIEHDTSVLGGKGSFPSGHAARASVIAFVLGLALSER
                     FPKGWLLLWIFPISVGLSRIYVLQHYPMDVIGGILFGMIIATILASKLSISKDTSKT"
BASE COUNT         2556 a         1138 c         1291 g         2352 t
ORIGIN      
        1 gattccaaat ttctttgcaa tttcatcgaa tggtttaatc accaatggaa attcccattg
       61 tatctcattt agaatttcag tgtcaagttt atccatatta gacaatatat cagatcttca
      121 ctatcaatta aaaaatgtaa ctagtttttt tgaatctata aatatcattc acggtgtgcc
      181 agattattga ttattgatct tcatctaaag gggaaccttg tgattgtgat aggttctgga
      241 aacgagggcg taaaaaaagt caattcgctt ttgacgcagg attgtaaaat tcttgtgata
      301 agtgataaat taaattccca aattgaaagg tatgtcaaac agggaaaaat taagtttaaa
      361 aaaactaaac tgaaagatgc gacatttctt tcaaaataca aaccctatct ggttatggct
      421 actacctctg acagaatgct aaacagaaaa attgttgaaa aagcaagaaa aatgaaatca
      481 tatgcgtatg catcagatga tcctgaaatt agtgactttg cacatccctc aatcatcaat
      541 atagctgaca cgatccaaat tgcaatatca acaggtggaa gtagccctgc gatggcaaga
      601 aagattaaac taaaggcaga accttttttt aaaaaaaata tctcaaacga ggatatctat
      661 caaatcaaac tgcaaaaatt cgcaaggttt gaagcaaaac aagtgctttc tacccaatta
      721 gatcgcaaaa aattcctcta tgcagtaatg aatgataaac gtgtaaaagg attattaaaa
      781 gaaggaaaat acaaaatgtc acaggtacaa gtaaaaaaaa tgcttaagga atggtcatga
      841 aacaaaatat aatcaatgca cgcataacat ttagaaaatc acctatacat gtacttgaga
      901 aatttgcctt caaagatctg tctgatgcat acttatcttt taagaaacat gctagcattt
      961 cagaatgtgt gataattcag acatgtaata gggttgaatt atttactgcg tcaaataatc
     1021 acgatgttca taaaatcaaa aaaacttggg catcactaac tgggcttgaa gaaaatgcgt
     1081 ttgcagatac cttagaaata tgtgataatc atgatgcgtt tgaacatctg ttaaaactta
     1141 cgtccggatt agaatcattg gttgttggtg aagaacaaat tcttggacag attaaagaat
     1201 caattacagt tgcaagaaat acaaaagctt ctggtgaaaa attgaataaa ttatttgata
     1261 catctattcg gattggaact cgtattagaa actcaacagg aattagtaaa ggaggtatat
     1321 cattaggttc aatggctgta aaattagctg aagaaaatgt agatgatata aaatcaaaaa
     1381 atgtcttgct aataggaaca ggagaagcag caactatggt tgccaaatct cttaacaaaa
     1441 gaggttatga gtttcatgta actagtagaa ctgtcgaacg atctactggt ttttctcaaa
     1501 cattaggtgg taaacccata aaatttgagg atgttttatc aacattcgat aactatgaga
     1561 taatttttgt tgcaactatt gcaccttatt tcttgattac ctttgataaa atgatagaat
     1621 cgatgaagaa taaaaaatct ggaatgatga tattggatct atcaaacccg agaacagttg
     1681 atgaacaagt tgctacaatc ccaggaatta aattgatgaa tattgatcaa attgctgaaa
     1741 tggtggataa aaatatgaca aaaaggaagg aaaaggtatc tactgtacaa aatataatta
     1801 gtgaagaagt accaatcatt gaggctacaa tgaaacgact tgatgcagaa ccactagtca
     1861 aagatgtatt tactaatgca gattatatga gaatcaagga gttgcaaaag gcgttacaaa
     1921 tgttaggaga aacagatgaa aaaagaatta aaattattga tgagttgaca aaagctgttg
     1981 tagagagtat tgtttctgct cctatgaata acttgagaaa ggcatcggaa caaggtaatc
     2041 cagatttgtt agaaacagca agtaagttat ttaattataa gaaaaaggat tagtcatact
     2101 aacctaagat tatattactt gttatcatat ttttatcatg gctacgtttc cctcaaaaag
     2161 attaaggcgg ctacgagtta atgaaaatat tagaagtttg gtgcaagagg tcagactgtc
     2221 tgtaaatgat ttagtatgcc caatctttgt agaagaagga ttaaaagaaa agaaatcagt
     2281 tggatcaatg ccagatattg tgagattacc attatcagag atttcaaatg aagtgcaaaa
     2341 tatttctgat ttaaaaatac cagcagtgat gctgtttgga attccttcac aaaaagatga
     2401 aagtggaaca tcggcatatg ctgaaaatgg tattgtgcaa aggacaattt ccgaaataag
     2461 aagcaatttt ggtgataaaa tatcgatcat ggctgatgtc tgtctatgtc agtatactag
     2521 ttcagggcat tgtggaataa taaatgggaa aaaaatagac aatgatctga gtataaagac
     2581 tttgactgat attgctgtta gtcaggcaaa gatgggtgtc gatgtggtct ctccatctgc
     2641 aatgatggat ggtcaagtat ctgctataag aaagggatta gatgatgcaa attttaaaga
     2701 tgtaataata atgtcacatt cagcaaaaca cagttcatcg ttttattcac cgtttagaga
     2761 tgcagcagaa tgtgctccac aatttggtga tagaaaaaca taccaagttc catttacaaa
     2821 tccacgtgaa gcgatgaggg aagttgaagc agatattgaa gaaggtgtag atatagttat
     2881 gataaaacct gcactatctt atcttgattt aattgcggaa gctaagaaaa aatttaatgt
     2941 tccagtttct gcatatagtg tctcaggcga atatgcgtta gttaaagctg cggcaaatca
     3001 aggatggatc aatgaaaatg aagtgacaaa tgaaatactt tcgtccatca agcgtgcagg
     3061 agctgatttc atcgtgacgt attttgctaa attaggtgca aaattaatta atgaaaattc
     3121 atgatagacg atgaacgttt tgagatacag gttgcttttg atttattacc acatgtagta
     3181 ggctgtagtt gggctactat ctggtttcgc ttaaataaaa ttaaaaatcc tacaagagaa
     3241 gaatttaggc aaaaagtgac cgagtatttc aaaatgcttg atccattggt gaatatatat
     3301 ccggaagaca aaaactttga ggaaattatc aattacataa gaaaacgaaa tgttgtagaa
     3361 attaaaaaaa taagtgctgg gacaaaccct gaagttgaaa gaagatatga cagatatgtt
     3421 gattatggct aaaatagcat caaattcgcc ataatttggg tctgacaaac tttaattttc
     3481 gttgtaataa gagagcctgc caatctatgc atgtaaaaaa tatgaccgag cctataccta
     3541 ccacatctgc tacaattatg cctattattc tatcctcaaa taattgtaaa aatggtacaa
     3601 gtacagcggc acttattgta atcatcacat aataagatag gagtcttcca aaccaaaatc
     3661 caatataaag tccaatactt tttgctctca tcattccata tgcaacaaat aacatgttac
     3721 taggaagtgg tgttactgca aatagaaaag atgtcagggt atatccaaat tttttacgat
     3781 tcaggaaatt gccgataaca tctagattcg atttttgttg tttacccaca aatcgacgga
     3841 aaaaaccact catccatttt aaaaagaatc tgccgattgt agctcctgtg gctcccacta
     3901 atgcaaggaa aaatggatcg agtgaagtat ccaatacaaa aaatgatgac agtatgatcc
     3961 aagtaggagg catcaaaata ggtgcagcat ttactgccat caacaaaaat aatatgccga
     4021 ggtaagaata ctccgcaatt acttcgatta tatctgccat aatagtaaaa ccagcactcc
     4081 atctaaaact cttaagaaag tatcaatcat accttaccct taattattaa tttaagtaat
     4141 atttctcatg agtaccaaag ctaggaaatg ttttagatgt aatttgatat tcataaatga
     4201 agaaattgcc atattgcata aggatctttt aaaccatcct acaaaactgg ttcgattaat
     4261 taaatcataa atcagtatca ctttttcatg taaaaatata aaaatgcagg aggtgggatt
     4321 cgaacccacg aacccctaag ggaaaggata acccataacc ttgatcttga gtccttctcg
     4381 gttggccggg cttcgatacc cctgcacatt gaattaatca tgtcgtttaa aaaaagttat
     4441 taattgaaga aatagagaat acctaatgac aattgaagat gcaaaaaacc agagatccga
     4501 tattaccctt caaggaacaa ttgaggaaaa gggtgaacca agaacagtca atactaaata
     4561 tggagaaaca caagtttgtg attcatattt gaaggatgac actggtagga taaaactcac
     4621 actttggggt gaggatatcg aaaaagtgaa gaatggcgac actgtttcga ttcaaggggc
     4681 atatacaaca actttcagaa atgaagttca gctaaatgtt cccaagaaaa atggaaaatt
     4741 agaagtaatt tctaattaat atctaataaa ctatctcaat ctagagatta ttttttattc
     4801 tttttagatg cttttcgttt tggtgctttc tttttagatg cttttcgttt tggtgctttc
     4861 tttttagatg cttttcgttt tggtgctttc tttttagatg cttttcgttt tggtgctttc
     4921 tttttagatg cttttcgttt tgatgctttc tttttttcgg ctagtatttt attcgcaagt
     4981 tttctagcca tactcaaaat tcgtttatcc tccacagatt ttctttttgc atctcttgct
     5041 tttttcttcc gtatgtttgc ctgactttct ttctttttgg atgtctgttc tttaattata
     5101 gaattaatct ttaagaaatt cttatgaaca ttttcttctg cctgtataag tgatggtaat
     5161 tttttttcta gttttgctac agttttttta ctttgttgaa tggctttcat gagttgtggt
     5221 tttgattcta gagtcctttt aatttgctta tttagtttag attttttaat atcatattct
     5281 tggattgact gaataacttt ttctaatgtt ttctttcttg atttaagttg agagtgtaaa
     5341 tcttcaattt cggtagaaac ggtatttatg gtaaattgca gttgtttttt tgcctcgcca
     5401 ctcgcaaaag atacctcttc cttaatttca tcatattttt ccttctcact agatagtcga
     5461 tcttctgctt gttcaatcaa acttgtgata ctgtcacgtt gagcaaattt ttgttgtaaa
     5521 acggtagata caccctctaa ttctgaatta aaattgtcca ttttatgctg gattgaaaca
     5581 atacctgata ctgatttttt gtatagtaat tttgctaatc tcaatgtctc tgatgtttta
     5641 gtacgatttt ttttggctct agatttcctc tgtttcagta ctgttaattt ttttgtcagt
     5701 gataccatat caaattagcg cttgatgagt cggttaaatt gtaaatatga ctagtaattg
     5761 acttgacggt taagaaattt tgcatgtaat tatgaaaatt ttgtcagata acgctggtgg
     5821 tattcttctg ctctataaaa atttgataca ggttcgatcc aagttacaat ttctttcttg
     5881 aatttttctt tcacaacaga atttagttga tccttcattt ctgttgctaa ttttttttgt
     5941 tcttcgctat gatagaaaat tatagatcta tactgcgatc ctacatcagg tccttggcga
     6001 ttcaatgttg ttggattatg attttcccaa aaaatcttta gtaaatcatt gtatgaggta
     6061 atttctggat cataatcaat ctctacagat tctgcatgac cagtagtatc tgtgcataca
     6121 tcctcatatg tgggattttg tttcattcca ccagaataac caacctgagt tgattttaca
     6181 ccttttgttt ttctaaatat ctcctcaaca tgccaaaagc aacctgcacc aaacgtggct
     6241 ttcatattat tttaataaca aatatctaat ttataattta gatggaaaag gctgacattg
     6301 aatcaatacc agttcaaaag acatttgatc taaaagatga aaaagatgct tatgatgcag
     6361 ctgaagaaat ggtacgaatt gggttttaca aagaaaagaa aggattcaaa gttttgatgg
     6421 caaaagagcc caaaaaaaca gccaagcgaa ttgggtatat agttacaaca accgtaactg
     6481 ctagtttgag gaatgatgat cagtatagag acatcagata ttggacatat catcatgaca
     6541 aggaacgtta tggtattgta ctggttaact ctaaagttat agatgaatta ggtttttgaa
     6601 gtatctttgg aaatagaaag cttgcttgct aatatagtag caattatcat tccgaacaaa
     6661 attcctccaa tcacatccat tggataatgt tgtaaaacgt aaatcctgct aagtcctact
     6721 gagataggaa aaatccataa aagaagccaa ccttttggga atctttctga tagagccaag
     6781 cctaaaacaa atgcaattac agatgcacgt gccgcatgtc cagatggaaa agaaccttta
     6841 ccacctagga cagatgtgtc atgttctatc tccagtggta actcagttcc aatgtattca
     6901 agatctggtc ttggacgctc aactatgtaa tctttgaaat aacctgatgc cacagtagca
     6961 ataattacag ctagtaacat taccaaaccc atacgtctag ttctacgcca tacaaacatt
     7021 acaaaacaga aaaccatgat tgggattatc ccaccaattt ctgtgattgc ccacattgat
     7081 gtatctaacg ttgagttacc agaaattgac tgaaaatatg ttatcacaga tatgtcaaat
     7141 tgttccgtga tctctgcata aactaatgca gtgataataa ggaatgatat aactaggaaa
     7201 aaaaaaccaa ttgaacgcat ttcgaagata aatttttgca tcaacgaact ctcataacga
     7261 tcatttttaa ttttgtctat gaattaagtg atcgtaccca aaatcagtgc tgttttttat
     7321 aaaacataaa cagaaca
//
LOCUS       DASCPJ010000068         3632 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001075763, whole genome shotgun sequence.
ACCESSION   DASCPJ010000068 DASCPJ010000000
VERSION     DASCPJ010000068.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001075763"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(221..562)
                     /locus_tag="QGF01_03065"
     CDS             complement(221..562)
                     /locus_tag="QGF01_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HJN19909.1"
                     /translation="MMRGGNRQMRRMMDKMGLDMTEIPNVQEVIIKTDKKEIIIPKPS
                     VTEMKSKENSIFQVIAESFEEKELEVQIFSEDDIMLVCQQASCNEDQAKEALGETKGD
                     IAQAILRITSN"
     gene            complement(569..1033)
                     /locus_tag="QGF01_03070"
     CDS             complement(569..1033)
                     /locus_tag="QGF01_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HJN19910.1"
                     /translation="MNSISKLKHTIDALFGTGVSKYLPKDIDILFSHRTGRIREVSHK
                     QKLLCTLRIDGGLAITPYFAQMLLASKRFKENCLEIDKESKPFVEDGRSVFCGHVISC
                     GKNIRIQSEVPVLYKNKVIAVGKAILSSEMMKGQTMGVALKIRDSLKSQQRG"
     gene            1124..2170
                     /locus_tag="QGF01_03075"
     CDS             1124..2170
                     /locus_tag="QGF01_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HJN19911.1"
                     /translation="MMSEEETRKIQFTGKSTYIVSLPKKWVGDLGLKKGDQILVQRKG
                     TSSLQIVPYSTRTKRESETATLEIDPKEQPSSVERKLIALYFLHFKTISIKPIKGRIT
                     PSQRTGIRNAVKKILMGSEITAESSDGITIQILINLVELSVVGALKRMLLLAKSMQND
                     ALLAIKEGNTELANEVINSDDDVDRFSFYITRQLAIAIENEHLLKEMGFSNARDCLGY
                     RLIIKNIERMGDHAVRISKDVLDYKKPISGKIFDAIRDMSDFAISVIDDASDALFQGD
                     IDRAEQAVTDAQNITKKEKRIMDYLKLNIDEEEKYRIRRIVENTRRIAEYASDVGEIV
                     LNMNIEKIIKKPKM"
     gene            2179..3288
                     /gene="hflX"
                     /locus_tag="QGF01_03080"
     CDS             2179..3288
                     /gene="hflX"
                     /locus_tag="QGF01_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HJN19912.1"
                     /translation="MDSAILITYDQDDVISETLALCDSAGFKVESVIKQDYLQKPKYG
                     ISEGKIQDLNDLIERTNPDVIVFDEVLKPSQHYNLASELKMEILDREALLLQIFEKRS
                     SSAESKLQVQLAQTRYEMSRAKEKVKLSRRGEQPGFMGLGTFEVDVYYNEIKRRMINI
                     KSKLAKSGKQRELHRQARKRLGFKIISLAGYTSAGKTTLFNRLTGEQKEENSALFTTL
                     STTIRKVMIGKETALISDTVGFINKLPAYMIEAFKSTLEELLYADIILVLIDANDGLY
                     QLEKKFKNCFRILNEIGVEPNKMIFVLNKSESLSDDEILSKANYLKFNENKKWMAISA
                     ITGKNINKLEKLINEYFQNNILENKNNVETKAYGN"
     gene            3278..>3632
                     /locus_tag="QGF01_03085"
     CDS             3278..>3632
                     /locus_tag="QGF01_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HJN19913.1"
                     /translation="MEIEVLRIGQRVVRDDRVTTHVALVARSFGAKKIYMNEINPEIN
                     DTVSKINKTWGGDFEIEMIDDWKKIIREKKNMATKIVHLTMYGQNINDIQTKLQNEDK
                     LLIVVGAEKVPRTIYD"
BASE COUNT         1322 a          542 c          625 g         1143 t
ORIGIN      
        1 gataaaatca gttcttcata atcttcccta gtagtatttt catctttttc tagaatttct
       61 ttcatttctt ttctactgac atcttctagt tccccgataa ttcttaaagc attttgtatt
      121 ctttccttat cgttcataac attctaagaa tatgctagtt gattaatttt aatttaattt
      181 tattccatat taatttggat gttatggtac gaaaaatcac tcaattactt gtaattctaa
      241 gtattgcctg ggcaatgtca ccttttgttt cacccaacgc ctctttagct tggtcctcat
      301 tacagcttgc ctgctgacat accaacatga tatcatcttc tgaaaatatt tgaacctcta
      361 attctttttc ttcaaaactt tctgcaataa cctgaaatat tgaattttct ttggatttca
      421 tctcagtaac agaaggcttg ggaataatga tctctttttt atcagttttt attattactt
      481 cttgaacatt tgggatctct gtcatatcaa gacccatttt atccatcatt cttcgcattt
      541 gccgattacc tcctcgcatc ataatgatct atcctctttg ctgacttttt aaactatctc
      601 taattttgag tgcgactccc attgtctgtc ctttcatcat ttctgaagat agaatagctt
      661 ttccaactgc aattacctta ttcttgtata atactggaac ctcactctga attcttatgt
      721 ttttaccaca tgatatgaca tgaccacaaa atacagatct tccatcctcc acaaatggct
      781 ttgattcctt atcaatttct agacaatttt ccttgaatct tttactagcc aaaagcattt
      841 gtgcaaaata tggagtgatg gctaaaccgc catcaattct taatgtacaa agaagctttt
      901 gcttatgaga tacttcacga attcggccgg ttctatgtga aaataaaata tcaatatctt
      961 ttggcagata ttttgaaact cctgttccaa ataaagcgtc aatggtatgt ttcaattttg
     1021 agattgagtt catcagaatg acgtatctag atatctaaat atttcttgac actacatcat
     1081 agaggtcaat taatctatat agaaggaaaa aaagaatata gatataatga gtgaggagga
     1141 aacgcgcaaa attcagttta cgggaaagtc tacctacatt gtatctctac caaaaaaatg
     1201 ggttggtgat cttggactaa aaaaaggtga tcaaattcta gttcagcgta aaggaacatc
     1261 tagtttacag attgtacctt atagtacaag aacaaaaaga gaatctgaaa cagctacact
     1321 agaaatcgac ccaaaagaac aaccgtcatc agtagaaaga aaactaattg cactttactt
     1381 tttacatttt aaaactatta gtataaaacc aattaagggt agaattacac cgtcacaaag
     1441 aactggtatt agaaatgctg ttaagaaaat attgatgggt tcagaaataa ccgcagaatc
     1501 ctctgacggt ataactattc agattcttat taatttagta gaactttccg ttgttggagc
     1561 tttgaagaga atgttattgt tagcaaaatc aatgcaaaat gatgctttac tcgcaataaa
     1621 ggagggaaat acggaacttg caaatgaagt tatcaatagc gatgatgatg tagatcgatt
     1681 ttcattttat attacacgcc aactagcaat agcaattgaa aatgaacatt tgttgaagga
     1741 gatgggattt agtaatgccc gtgattgtct aggttacaga cttatcataa aaaatattga
     1801 aagaatggga gaccatgcgg ttagaatatc aaaagatgtc ttggattata aaaaaccaat
     1861 aagcggtaag atttttgatg cgatacgtga tatgagtgat tttgcaattt cagtcataga
     1921 tgatgcaagt gatgcattgt ttcaaggaga tattgatcga gcagaacaag cagtgactga
     1981 tgcacaaaat attacaaaaa aggaaaaacg gattatggat tatctgaaac taaatattga
     2041 tgaagaagaa aaatatagga ttagaaggat tgttgaaaat acacgaagaa tagctgaata
     2101 tgctagtgat gttggagaaa ttgttctaaa tatgaatatt gaaaaaatta tcaaaaaacc
     2161 aaagatgtag gtttggctat ggattctgca atattaatta catatgatca ggatgacgta
     2221 attagtgaaa cacttgctct ttgtgattcg gcaggtttca aagttgagtc tgttataaag
     2281 caggattact tacagaagcc aaaatacggt ataagtgagg gtaaaattca ggatctaaat
     2341 gatctcatag agagaactaa tccagacgtc atagtttttg atgaagtgtt aaagcctagc
     2401 cagcattata atctggcttc tgaattgaaa atggaaatac tagatcgaga agctttactt
     2461 ttacaaatat ttgaaaaaag atcttctagt gcagaatcta aacttcaggt acaattagca
     2521 caaacacgtt atgaaatgtc aagagcaaaa gaaaaagtca agctttcaag acgtggggaa
     2581 caacctggat ttatgggcct tgggacattt gaagttgatg tgtattataa tgaaattaag
     2641 aggagaatga ttaacattaa atcaaaatta gcaaagtcag gaaaacagcg tgaattacat
     2701 agacaagctc gaaaacgtct gggttttaaa ataatttcat tagcaggata cacatcagct
     2761 ggaaaaacta cattattcaa tagattaact ggagagcaga aagaagaaaa cagtgcatta
     2821 tttactacac tttcaactac tatcagaaaa gtaatgattg gtaaagagac ggcattaatt
     2881 tcagatactg ttggatttat caacaaactt ccagcataca tgattgaagc tttcaaatca
     2941 acattagaag agcttctgta tgctgatatc atactagtat taattgatgc aaatgatgga
     3001 ttatatcaat tagaaaaaaa attcaaaaac tgcttcagaa ttctcaatga gattggcgtg
     3061 gaaccaaaca agatgatttt tgttttaaac aaatcagagt cattgagtga tgacgaaatt
     3121 ttatctaaag caaattactt gaagtttaat gaaaataaaa aatggatggc tatttcagca
     3181 attactggaa aaaacattaa caaacttgaa aaactaatta atgaatattt tcaaaataat
     3241 atattagaaa acaaaaataa tgttgagacg aaagcgtatg gaaattgaag tgttaagaat
     3301 tggtcaacgg gttgtaagag atgatagagt tacaactcat gtagcccttg tggcaagatc
     3361 atttggagct aagaaaatat acatgaatga aattaatcct gaaataaatg acactgtttc
     3421 taaaatcaat aaaacatggg gcggtgattt tgaaattgaa atgattgatg attggaaaaa
     3481 aataatcaga gaaaagaaaa atatggctac aaaaattgtt catcttacta tgtatggtca
     3541 aaatatcaat gacattcaaa ctaaactaca aaatgaggat aaactcttga ttgtggttgg
     3601 agcggaaaaa gttcctagaa caatctatga ta
//
LOCUS       DASCPJ010000069        11990 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001086658, whole genome shotgun sequence.
ACCESSION   DASCPJ010000069 DASCPJ010000000
VERSION     DASCPJ010000069.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001086658"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="QGF01_03090"
     CDS             complement(<1..692)
                     /locus_tag="QGF01_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HJN19914.1"
                     /translation="MSSTSAAGQRCPSCTKTKMILDDNTGELFCSFCGFVVTDKIVEG
                     GPEWRSFSNDGPDRSRVGSGTSITMHDMGLSTIIGPQNKDATGKPLDTNMKKSIERLR
                     TWDNRSQAYSSSDRNLRQALSEMDKLQDKLSLTNAVIEKAAYIYRKAIERKLVKGRSI
                     HGLVAACIYAACRNTETPRTLDDVANGINIRRKDVARCYRLIFRELDLKIPVADPVKG
                     VSRIASIAGLGE"
     gene            complement(824..2353)
                     /locus_tag="QGF01_03095"
     CDS             complement(824..2353)
                     /locus_tag="QGF01_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19915.1"
                     /translation="MIKNLMYTLIAISLILATSTGTVFADDIDVKGTVHDLTIHANWT
                     GELYDAKQNFMKGDYKKSINIYGDYLEYFPNDVNIKAMKGIALNNLRLGSTLASQPSE
                     NFSFPSNPVHLNKLSMLEFYEILKLEPHNIIALNGMGLGFGNYAEYEESKKYFKKTLD
                     VKPDDKIAKNYIDYIEKVEKKFITSSTEKPEFLKQLEKKEIPDWVKNNAEWWADEQIS
                     DSDFIYGIEYLIQKSVIIINTVNINESTSEHIPSWIKINASWWAKDIISDRDFLSSVQ
                     YLIENGIIKLNYYNDSENLKRDQDRQRWNFQLYLDNITKRLKDEIIYVEHPNPSADVI
                     KKFHRDSSKWSAPGLDKQQQFPDREISLIDDIYHVKYKIFVSPQPAGLPLDHVGTLKN
                     SFKYWEGRTFVASDDNEVIFHLEITERSDLANIWVTWSVRSLGEGILGHANIGKGVAE
                     VALGSYGCDGSFQLFDIETVEKIMTHELGHSIGFGGHSDDPNDIMYSSMSPGFAYCLL
                     D"
     gene            complement(2401..3420)
                     /locus_tag="QGF01_03100"
     CDS             complement(2401..3420)
                     /locus_tag="QGF01_03100"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HJN19916.1"
                     /translation="MSKIKLKNMYNSFEKTSISALLLLLKSHQTEYLSGQDLSDVLKI
                     SRVAIWKHIKKIRSLGYKIESKQHLGYKLTDFTDQLIPWEITDNLRTKLLGKRVYYFE
                     TIDSTQAFAIKIAANKIENGTVVISKRQSKGRGRLKRKWESPNGGIWMSVIIHPKFDI
                     NHVMLMPIATSLALCLAIEKILKIKPELKWPNDLTIHRKKVAGILLDSSIQSNNIEYL
                     VLGIGINFKIKQKKLENMLKKQSNFYGVQTLTKKNERPITLVKEFLCELENILHMINL
                     DQNKKIVNMWTKRSSTIGKKIVYMTDNGKKIGKVIKMDHDGALIISSNKKRERLIAGD
                     ISYKN"
     gene            complement(3437..4087)
                     /locus_tag="QGF01_03105"
     CDS             complement(3437..4087)
                     /locus_tag="QGF01_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJN19917.1"
                     /translation="MTDFLIRQDHKKLEIKSKEKIIKFLRSQQTGRIASIDESGFPQI
                     IPMNFVFINDTVYLHSHIRGEKLDNIRRNQKVGFEVDKSLEFLPSYFSDPTDASLADT
                     LYISVVIKGNASIVSNKEEKTIALNGLMKKYQPEGGYEPIKPDMDVLKGVEVIKIVPG
                     SLRGKYKIGQNMDMKSRIDLARQILERNSHTAKETLDVMGFEIINNELKLVDDTPW"
     gene            complement(4119..5204)
                     /locus_tag="QGF01_03110"
     CDS             complement(4119..5204)
                     /locus_tag="QGF01_03110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJN19918.1"
                     /translation="MIKFGIQNGLNVARAGYSEDQILTACMLADRTGYDSIFYMDHTN
                     VPQWKNAIVLDPWVMLSSIAAVTNHVELGTCVTDAIRRHPSNIALAAITLDRISKGRA
                     ILGIGAGEAQNLKEFCIPFEKPVSKWEEQIQVIKKLYHSTPDNTVDFEGKYYQLESAC
                     LQAPPIRKPHPPTYMASGGKRTLYLTGKYGDGWLPIGYTPELFEDHKKQIEVSMNENN
                     RTQEEKDNFQMALDIDVYFSDDAEESWAKMKEAVKVSLFKPEILRVHGLKEIEGFDFR
                     KYFTEYSMSNQDWIVKMREAATKIPDGIARSSTGVGTPDDIIPIFERFMKAGVNHFVI
                     RFWGKNYFGSIDKFASKIIPYFKEQQK"
     gene            complement(5293..6237)
                     /locus_tag="QGF01_03115"
     CDS             complement(5293..6237)
                     /locus_tag="QGF01_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HJN19919.1"
                     /translation="MHTVRIPKVIQFGENALSEADYPKNALVVTTAPPALSGKWLDKM
                     GIQDYMLFDKVTPEPSIDDVKNLIDEFKTKNPSALIGLGGGSSMDVVKYSSSEFGVEK
                     ILIPTTFGTGAEMTTYCVLKFDGKKKLLHEDRFLADRAIVDSYFMDGTPEQIIKNSVC
                     DACAQATEGYDSKLGNNLTKTLCKQAFDVLYDAIMNDKPENYPYGSMLSGMGFGNCST
                     TLGHALSYVFSNEGVAHGYSLSSCTTIAHKHNNSSFYEKFKQVIEKLGFDKMQLKAPV
                     DEAADVVMTDKGHLDPNPIPISKDDVIKCLNAINDGNL"
     gene            6320..7600
                     /locus_tag="QGF01_03120"
     CDS             6320..7600
                     /locus_tag="QGF01_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HJN19920.1"
                     /translation="MIKNFDLLANTKMERKALQILESGLMAAQPKNFLKKSVHQNHIS
                     LGKNRIVLSKYRKIFVVAYGKAASTMTEYVVKKINISKGIVVIPKNTKSSLISKKFII
                     IHAGHPIPDRESVKAGKIIQKFVESCSKEDFILFLVSGGGSSLLSLPDKITLDEKKQV
                     TKLLLKSGATIDEFNSVRKHLSRIKGGKLVKNMQSDGCALIMSDVLTNNLSVISSGCT
                     YNDNTTFSDAIKVITKYSLTKKLPKKVIAHLKHGLKEQVVQPKRLKVKNEIIATNLDC
                     LNTMAMKAKSLGISTKIYSSVNDDVSTSAQKIVKTMKSSRKSCIIFGGEPTVHVKGNG
                     KGGRNQELVLHILKRIQNIDQNVLVSSISTDGIDGNTNYSGALIKNHSINLQKISLYL
                     KNNDSNSFFKKYGGLIKTGPTNTNLMDVGLIIKY"
     gene            complement(7602..7808)
                     /locus_tag="QGF01_03125"
     CDS             complement(7602..7808)
                     /locus_tag="QGF01_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19921.1"
                     /translation="MKDINDVLPKVPNMKWGALMNKAPTNDKVEELNKIFPANGKWHT
                     VFEETDHTYIDGNLIWKKDKKDWT"
     gene            complement(7940..8383)
                     /locus_tag="QGF01_03130"
     CDS             complement(7940..8383)
                     /locus_tag="QGF01_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19922.1"
                     /translation="MEHELFFDGNLKEYSWSIKTGKEIINQIREHPAAYLSGGKLDIK
                     NKEESKFVALHVGIYWGLGVFIIKDFDKIHVMCDTKEMHEILIQQHKTQNQIIDDKIH
                     FINQLVGHRNLKLEYHLIESAKNIATEHLSGTNKTSASRDSSGFV"
     gene            8460..8666
                     /locus_tag="QGF01_03135"
     CDS             8460..8666
                     /locus_tag="QGF01_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19923.1"
                     /translation="MQESHSFFTRMVDELVEFSEHDAELSDGIKWLDNQAQKKGLSFY
                     DMVFEVLYKHDVNSKAKEWLNSRN"
     gene            complement(8673..9839)
                     /locus_tag="QGF01_03140"
     CDS             complement(8673..9839)
                     /locus_tag="QGF01_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HJN19924.1"
                     /translation="MDTKNIGLRNIEVADTKISCIDGQKGKLIYRGFDILDLAKNSNF
                     EETCYLLLHDELPTKNEYDDFKKKLSDSRLIPKQMQKNMGNWRKDADPMDVLQAFVAA
                     FGGYYDEEFSNKESSYERAANLIAKVPTVVASWSRIRNNEKIIDPDHSLSHAGNFLYM
                     LNGKKPESEIEKIFDICLILHADHTLNASTFAAREVASTRAHMYSAASAAVGALSGEL
                     HGGANYEVMKMLLEIETEDNVTPYIKEKFKKGERVMGMGHAVYKTVDPRSQILKEFSK
                     RLSKKTGQPWYDITSKVEEVTASLMKESKGTEIFPNVDLYSASIYYMLGIPMDLNTPI
                     FAVSRVAGWAAHIIEEKFAEAAPKPMLYRPKAVYVGKYAGPQGCEYIPIEKRIN"
     gene            9954..10118
                     /locus_tag="QGF01_03145"
     CDS             9954..10118
                     /locus_tag="QGF01_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19925.1"
                     /translation="MSQEERKLMKLIDEHILNATPEELIKLQQVDLNTQLDCVWFYDT
                     YNLPENSIKA"
     gene            10187..11221
                     /locus_tag="QGF01_03150"
     CDS             10187..11221
                     /locus_tag="QGF01_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HJN19926.1"
                     /translation="MVIKSKKSDMNKISKSKKTERTKIICVSHQEDADGISSAALIKQ
                     VFGGETILVDYPGMMDVLESLRTDTKLKKLFICDLGLNKKTNDAFVDLLTELRKKRIS
                     VTYIDHHDMESKVINKLKKIKVKLIHDTSECTSVLVYNMLKKKLSENATFIAVCGAIT
                     DYMENKPISSKLLQMYDRQFALISATVLTYNIVGHQKDPDYLLYLVDELSESKFPHEI
                     PNTYEFAQIQVGKLAEIMSKVRKSMKVSKNLAHMEVLDSGASGAVNFVLGFSGKDVGI
                     AYKERVDKGIYAVSVRGSPSCKTHLGRLVSSVTSEIGGSGGGHDKACGAVIPKDKIKE
                     FLKKMNSELG"
     gene            complement(11213..11671)
                     /locus_tag="QGF01_03155"
     CDS             complement(11213..11671)
                     /locus_tag="QGF01_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HJN19927.1"
                     /translation="MIIEEKLKSLGIELPHPPKPAGSYIPVSIIKDLAYVSGQIPMLN
                     GKLRYTGKIPTPRTVEDGKSSAKLCAINALSQLKVKIGDLDRIVKIVRVSGFVNSSPD
                     FTEHAQVIDGASDFLYQIFAEKGVHTRTAVGVSSLPLDATTEIDMILELS"
     gene            11795..>11990
                     /locus_tag="QGF01_03160"
     CDS             11795..>11990
                     /locus_tag="QGF01_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN19928.1"
                     /translation="MNLAPQELERNASKSASDAIKYDSQGARGMAISHYEKAIQSLSK
                     LMKLYPDSKLNTIYTERLKSY"
BASE COUNT         3844 a         2063 c         1755 g         4328 t
ORIGIN      
        1 atttcaccaa gaccagctat actagcaatt cttgaaacac cttttacagg atctgctaca
       61 ggtattttta gatcaagttc cctgaaaatc aatctataac atctagccac atcttttctt
      121 cttatattga tcccatttgc aacatcgtct aatgttcttg gtgtttctgt atttctacat
      181 gcagcataaa tacacgcagc aactaatcca tgaatagatc ttccttttac aagctttctt
      241 tctattgctt ttctgtaaat atatgcagcc ttttcgatga ctgcattggt taaagatagt
      301 ttatcttgta atttatccat ctcactcaat gcctgacgaa gatttctatc tgatgaagag
      361 tatgcttgcg aacgattatc ccatgttctg agtctttcaa tagatttctt catgtttgta
      421 tctaatggtt tgccggttgc atctttattt tgtggaccaa taatagttga aagacccata
      481 tcatgcatag taatagatgt accagatcct actctacttc tatctggacc atcattagaa
      541 aatgagcgcc attcaggccc tccctctaca attttatcag ttactacaaa cccacaaaaa
      601 ctacagaata actcacctgt attatcatcc aaaatcattt ttgttttagt acatgatgga
      661 caacgttgtc cagctgcaga tgtgctactc atatactata ttagcgattt tattatataa
      721 ataaaatctg ctactttttt tggttgtttt gtgtgtagga cgagctaatt tactaatctt
      781 agcaatattg atagtaaatc acttctacat tatattttca tatttaatca agtaaacaat
      841 aagcaaaccc aggcgacata ctactataca ttatatcatt tgggtcatca ctatgcccac
      901 caaatccaat agaatgtccc aattcatgcg tcatgatttt ttctacagtt tcaatatcaa
      961 aaagctgaaa actaccatca catccataac taccaagagc aacttctgct acacctttac
     1021 cgatattcgc atgacccaaa ataccttcac ctagacttcg tacgctccat gttacccaaa
     1081 tatttgctaa atcacttctc tcagtgattt caagatgaaa aataacttca ttatcatcac
     1141 tggcaacaaa tgtacgacct tcccaatatt tgaatgaatt tttcaatgta ccaacatgat
     1201 caagtggtaa gccagcgggt tgaggactca caaatatctt atattttaca tggtaaatgt
     1261 catcaattaa cgatatttca cgatcaggaa attgttgttg cttgtccaaa cctggcgcgc
     1321 tccatttcga gctatctcta tggaattttt ttattacatc agcacttgga tttggatgct
     1381 caacgtatat gatttcatct ttcaatcttt tagtaatatt atcaagataa agttgaaagt
     1441 tccatctctg tctatcttga tctcttttta ggttttcaga atcattataa taatttagtt
     1501 ttataattcc attttcaatt agatactgta cactagataa aaaatctcgg tctgatatta
     1561 tgtcttttgc ccaccagcta gcattgattt ttatccaact gggtatatgt tcggaagtgc
     1621 tttcattaat attaactgta ttaatgatga taacactttt ttgaataaga tattcaattc
     1681 cataaataaa atccgagtca gaaatttgtt catccgccca ccattcagca ttatttttta
     1741 cccaatcagg tatttctttt ttttctaact gtttaagaaa ttcaggcttt tcagtagagc
     1801 tggttataaa ttttttttct accttttcga tataatctat ataattttta gcaattttat
     1861 catctggttt tacatccaaa gtttttttga aatatttttt agattcttca tattcagcat
     1921 aatttccaaa tcctaaaccc ataccattta acgctatgat gttgtgtggt tcaagcttta
     1981 gtatctcata aaactctaac attgacaatt tgtttaaatg aactggattt gatggaaagg
     2041 aaaaattttc acttggctgt gatgccaatg ttgagcctaa tctcaaatta tttaatgcga
     2101 ttcccttcat tgccttaata tttacatcat ttgggaaata ttctaaataa tcaccgtaaa
     2161 tatttatgga ttttttataa tcccctttca tgaaattttg ttttgcgtca tataattcac
     2221 ctgtccagtt tgcatgaatt gtaagatcat gtacagttcc ttttacatca atatcatctg
     2281 caaatactgt accagtagat gttgctaaaa ttaatgaaat agctatcaat gtgtacatta
     2341 gatttttaat catatttgtg ataagcacca attttacaaa atatattaaa agtatatcta
     2401 ctaattcttg tatgatatat ctccagcaat caatcgttct ctttttttgt tggaagaaat
     2461 aattaatgca ccatcgtgat ccatcttaat tactttacca attttcttac cgttatcagt
     2521 catgtacact attttttttc caatggtaga tgatcttttt gtccacatat taacaatttt
     2581 tttattttga tccaaattaa tcatatgtaa aatattttcc aattcacaca aaaattcttt
     2641 aactaatgtt ataggccttt catttttttt tgttaatgtt tgtacaccat aaaaattaga
     2701 ttgttttttt aacatgtttt ctaatttttt ttgtttgatt ttaaaattaa ttcctatacc
     2761 taaaactaag tattcaatat tatttgattg aattgatgaa tctaataata tccctgcaac
     2821 tttttttcta tgaattgtaa gatcatttgg ccattttagt tcaggtttaa tttttaatat
     2881 tttttctatc gctaagcata atgcaagaga agttgcaatt ggcattaaca taacgtggtt
     2941 aatatcaaat tttggatgta tgattacaga catccaaatt cctccattag gagattccca
     3001 ttttctcttt aacctacctc tacctttact ctgtcttttt gatataacaa ctgttccatt
     3061 ttcaatttta tttgccgcta tttttatagc aaatgcttga gttgaatcaa ttgtttcaaa
     3121 ataataaact ctttttccca acaattttgt tcttagatta tcagttattt cccatgggat
     3181 tagttgatct gtgaaatcag ttagtttgta tcctaaatgc tgtttagatt ctatcttgta
     3241 acctagtgaa cgaatttttt tgatatgttt ccagatagca acacggctaa tttttagaac
     3301 atcactcaaa tcctgtcctg ataaatattc agtttggtgt gatttcaaaa gtaaaaggag
     3361 tgcacttatg cttgttttct caaatgaatt atacatattt ttcaatttaa ttttgctcat
     3421 aaaataaaga tttagactac catggggtat cgtctactaa ttttagctca ttgtttatga
     3481 tttcaaatcc cataacatcc aatgtttcct ttgctgtatg tgaatttctt tctaagattt
     3541 gtcttgcaag atcaatacgt gacttcatgt ccatgttctg accaattttg tatttccctc
     3601 ttaatgatcc tgggacaatt ttgataacct caactccctt tagaacatcc atgtcgggtt
     3661 taattggttc atatccgccc tcaggttggt attttttcat cagaccattt agcgcaatag
     3721 ttttttcttc tttatttgaa acaatactgg catttccttt tattacaaca ctaatgtata
     3781 atgtatcagc gagagacgca tcagtaggat cagaaaaata tgatggtaaa aactccagac
     3841 ttttatctac ttcaaatcct actttttgat tacgcctaat gttatcaagt ttttcgcccc
     3901 ttatgtggga atgcaggtaa accgtatcat tgatgaatac aaaattcatt ggaatgattt
     3961 gtgggaatcc actttcatca atacttgcta ttctcccagt ttgttgtgat cttaaaaatt
     4021 tgataatttt ttcctttgat tttatttcta actttttatg atcttgtcgt atcaaaaagt
     4081 ctgtcataat gtatttaaca taaataattg aattgtattt acttttgctg ttctttaaaa
     4141 taaggaatta tcttactagc aaatttatcg attgaaccaa aatagttttt accccaaaac
     4201 ctgattacaa aatgattcac gccagctttc ataaatctct caaatattgg aattatatca
     4261 tcaggtgtac caacaccagt tgatgatctt gcaataccat ctgggatttt tgttgcagct
     4321 tcacgcattt ttacaatcca atcttgattt gacatggaat attccgtaaa gtatttccta
     4381 aaatcaaatc cttcaatttc tttaagtcca tgcactctaa gtatttcggg cttaaacaga
     4441 ctgactttta cagcttcttt catcttagcc caagactctt ccgcatcatc agaaaagtaa
     4501 acatcaatat ctaatgccat ctgaaaatta tctttttctt cttgtgttct attattttca
     4561 ttcattgaca cctcaatttg ctttttatga tcctcaaata attctggtgt gtaccctatt
     4621 ggaagccagc catcaccata tttaccagta agatacagtg ttctctttcc accagaagcc
     4681 atgtatgtag gtgggtgagg ttttcgtatt ggcggagcct gtaaacatgc agattctaat
     4741 tgataatatt taccctcaaa atctacagtg ttatccggtg ttgaatggta tagttttttg
     4801 ataacttgaa tctgttcttc ccattttgaa acaggttttt caaatggaat acagaattct
     4861 tttaagtttt gggcttcacc agctccaatt cccaaaattg ctcttccctt tgaaattcta
     4921 tcaagtgtga ttgcagctag ggcaatattt gatggatgtc ttctaattgc atctgtgaca
     4981 catgttccta gttcaacatg atttgtaaca gctgcaattg atgataacat gacccatggg
     5041 tctaagacaa ttgcattttt ccattgtggt acatttgtgt ggtccatata gaaaatagaa
     5101 tcatagccag tacggtctgc cagcatacaa gcagtcagaa tttgatcttc tgaataaccc
     5161 gctctagcaa cgttgagacc attttgaatt ccaaacttga tcattgttta ctcacgatat
     5221 taatcttgaa ctttacagtt aataagtttc acagaaataa gtcatagcta attatattcc
     5281 agtacaaaaa aatcataaat taccgtcatt aattgcattg aggcatttaa tgacatcatc
     5341 ctttgagatt ggaattgggt taggatcaag atgtccttta tctgtcatta caacatcagc
     5401 tgcctcatca acaggagcct ttaactgcat tttgtcaaac ccaagttttt caataacctg
     5461 tttaaatttt tcatagaaac ttgaattgtt atgtttatgc gctatggtag tacatgaaga
     5521 taatgaatat ccatgtgcaa cgccttcatt tgaaaataca tatgataatg catgtcctaa
     5581 tgtagtagaa cagtttccaa aacccatacc tgaaagcatt gaaccataag gataattttc
     5641 tggcttatca ttcatgattg catcatataa tacatcaaat gcttgtttgc ataacgtttt
     5701 tgtcaaatta ttacctaatt tactatcata tccttcagta gcctgtgcac atgcatcaca
     5761 aaccgaattc ttaataattt gttctggtgt accatccata aagtatgaat caactatagc
     5821 acgatctgct aagaatctat cttcatgtag aagttttttc ttaccatcaa attttaaaac
     5881 acaataagtt gtcatctctg cacctgttcc aaaagttgta ggtatcaaaa tcttttctac
     5941 gccaaattct gatgatgaat attttacaac atccatagaa cttccaccac caagaccaat
     6001 caatgcagat ggattttttg tcttgaactc atctatgaga tttttaacat catcgatgga
     6061 aggttcaggt gtaactttat caaataacat atagtcttga attcccattt tatccaacca
     6121 ttttccagat aatgctggag gagcagttgt taccactagt gcattttttg gataatcagc
     6181 ttcggatagc gcattttcac caaattgaat cacttttggt attcttactg tatgcatgac
     6241 ttattgaaat attaattatt attatacctt acaattatca tggctttggt ttatttattt
     6301 aaaataaaat tataaagcga tgatcaaaaa ttttgacctg ttagcaaata caaaaatgga
     6361 aagaaaggca ttacagattc tagagtcagg attaatggca gcacagccaa aaaacttctt
     6421 aaaaaaatct gtacaccaaa accatatttc acttgggaaa aatcgtattg tattatcaaa
     6481 atataggaaa atttttgttg ttgcatatgg caaagcagct tctacgatga cagaatatgt
     6541 tgtaaagaaa atcaatattt caaaaggtat agttgtaatc ccaaaaaata ctaaatcatc
     6601 actaatcagc aaaaaattta tcataattca tgctggacac ccaataccgg atagagaaag
     6661 tgtgaaagca ggaaaaatca tacaaaaatt tgttgagagt tgttctaaag aagattttat
     6721 actttttttg gtgtcaggtg gaggttcctc attattgtct cttccagata aaataacttt
     6781 agatgaaaaa aaacaggtga ccaaattatt attaaaatcc ggtgccacaa ttgatgagtt
     6841 taattcagta agaaaacatc tttcaaggat aaaaggcggt aagcttgtaa aaaatatgca
     6901 atctgatggc tgtgcattaa ttatgtcaga tgttctgact aacaatttat cagttatttc
     6961 atcaggttgc acttataatg ataatacaac attttctgat gcaataaaag tcataacaaa
     7021 atactcactc acaaaaaaac ttccaaaaaa agtgattgca catctaaagc atggtttgaa
     7081 ggagcaagta gtacaaccaa agaggttaaa agttaaaaat gaaattattg caaccaatct
     7141 tgactgcttg aatactatgg cgatgaaagc taagtcactt ggaatcagta ccaaaattta
     7201 ttcatcagtt aatgatgacg ttagtacgtc ggcacaaaaa attgttaaga cgatgaagag
     7261 tagtagaaaa tcttgcatca tatttggtgg tgagccaaca gtacacgtga aaggaaatgg
     7321 aaagggtggc agaaaccaag aattagttct acatatttta aaacgaattc aaaatattga
     7381 tcaaaatgta ttggtttctt caatttcaac tgatggaata gacggtaaca caaactattc
     7441 tggagcactg atcaaaaacc attcaatcaa tttgcaaaaa atttctttat atttaaaaaa
     7501 taatgattct aattcatttt tcaaaaagta tggaggttta ataaaaacag gaccaactaa
     7561 tacaaattta atggatgtag gattgattat aaaatattag tttatgtcca atcctttttg
     7621 tccttcttcc aaataagatt accatcaatg tatgtgtgat ccgtctcttc aaaaactgta
     7681 tgccatttac catttgctgg aaaaatttta ttcagttctt caaccttatc attggttgga
     7741 gccttattca ttaatgcacc ccatttcata ttgggaactt ttggtaatac atcattgatg
     7801 tctttcatgt atcgcaattt ttacccctat atttaaaatc ccttatcaat ttttacggtt
     7861 atttcaattg tttggaggca aagttctcat tactgtaatt agatgatttc caatcaattt
     7921 tcttcgtttt attaaattat taaacaaaac cactagagtc tctagaagca gacgttttat
     7981 tcgttccaga aagatgttct gtagctatat tttttgctga ctctatcaaa tggtattcta
     8041 attttagatt cctgtgccct actaattgat taataaagtg aattttgtca tcaattattt
     8101 ggttttgcgt tttatgctgc tgtattaaaa tttcatgcat ttcttttgta tcacacatga
     8161 catgtatttt gtcaaaatct tttatgatga aaacaccaag accccaataa attcccacat
     8221 gaagtgcaac aaattttgat tcttctttgt ttttaatatc taattttcca cctgaaagat
     8281 atgctgcagg gtgttctcta atttggttta ttatttcctt tccagttttt attgaccatg
     8341 agtattcttt caaattaccg tcaaaaaata attcatgctc catatgtcaa tcaaaagggt
     8401 gttttttgtc tatatatttt gaattaattc cttattgatc cattctgaaa ctttttgcta
     8461 tgcaagaatc acattcattt tttaccagaa tggttgacga attagttgaa ttttcagaac
     8521 atgatgcaga attatccgat ggtataaaat ggctggataa ccaagcacag aaaaaaggat
     8581 tatcatttta tgatatggtt tttgaggttt tatacaaaca tgatgtaaat tccaaagcaa
     8641 aagaatggct taattcaagg aattaatatt tcttagttga ttcttttttc tattggaata
     8701 tattcacatc cttgtggtcc tgcatatttt ccgacgtaaa cagctttagg tctatagagc
     8761 ataggttttg gtgctgcctc ggcaaatttc tcttcaatta tgtgtgcagc ccaccctgct
     8821 actcttgata ctgcaaatat tggtgtatta agatccattg gtatccctaa catgtaataa
     8881 attgaagcac tgtaaagatc aacatttgga aaaatctccg ttcctttact ttctttcatc
     8941 aaacttgctg taacttcttc aactttactg gtgatgtcat accaaggttg accagttttt
     9001 tttgatagtc gttttgaaaa ttccttaagt atttgtgatc ttggatctac ggttttgtat
     9061 actgcatgac ccatacccat aactctttca cccttcttga atttttcttt aatgtaagga
     9121 gttacattat cttctgtttc tatttcaaga agcattttca ttacttcata gtttgcccct
     9181 ccgtgtaatt ccccactcaa tgcaccaact gcggcactag ctgcagagta catatgtgcc
     9241 cgtgttgatg cgacttccct tgcagcaaat gtagatgcat ttaatgtatg atctgcatga
     9301 agaatgagac aaatgtcaaa tattttttca atttctgatt ctggtttttt accatttagc
     9361 atgtacaaaa aattaccagc atgacttaga ctatgatcag ggtcaatgat tttttcatta
     9421 ttacgaattc tagaccagct tgcaacaaca gttggtactt ttgcaataag attagcagct
     9481 cgttcataac tggattcttt attagagaat tcttcatcat aatagccacc aaaagcagca
     9541 acaaatgcct gtaagacatc cattgggtca gcatcttttc tccaattacc catattcttt
     9601 tgcatttgct ttgggattaa ccttgaatca gataatttct ttttaaaatc atcatattca
     9661 tttttagttg gtaattcatc atgtagtaaa agataacatg tttcctcaaa attagaattt
     9721 tttgccaaat ctaaaatatc aaatccacga taaatcagct ttcctttttg accatcaata
     9781 caggaaattt tggtatccgc gacttcaata tttcttaaac cgatattttt ggtatccata
     9841 ctttacattg aaaatagcat ttttaaattt tcgaccttga attcgtcaaa atttagcaat
     9901 ttgtctaata gattaacttc gagaattata tgatcaattt taactaaatc aatatgtcgc
     9961 aggaggaacg taaattgatg aagttgatag atgaacatat tttgaatgcg acaccagagg
    10021 aattgattaa attacaacag gttgatctta acacacaact tgactgtgtc tggttttatg
    10081 atacatataa tcttccagaa aattcaataa aagcttgata ttttacctaa attttttgat
    10141 cacatgtaat actaaattta aatgagggtt ttagtgattt aatcatatgg taatcaaatc
    10201 taaaaaatca gatatgaata agatatccaa atctaaaaaa acagaaagga ctaaaattat
    10261 ttgtgtatca catcaagaag atgctgatgg tatttcttct gctgcattaa ttaaacaagt
    10321 atttgggggt gagaccatac ttgtagatta tcctggtatg atggatgtat tagaatcgtt
    10381 acgaactgac acaaaattaa aaaaattatt tatctgtgat ttaggcttaa ataaaaaaac
    10441 taatgatgct tttgttgatt tgcttactga attaagaaaa aaacgaattt ctgtaactta
    10501 cattgatcat catgatatgg aatcaaaagt aatcaataaa ctgaaaaaaa ttaaagtgaa
    10561 attaattcat gatacttctg agtgtacttc ggttcttgtt tataatatgt tgaagaaaaa
    10621 attatccgag aatgctacat ttatcgctgt atgtggagca ataacagatt atatggaaaa
    10681 taaaccaata tcttcaaaac ttttacaaat gtatgataga cagtttgcat taatcagtgc
    10741 tactgtattg acttacaata ttgttggaca tcaaaaagat cctgactatc ttctttactt
    10801 ggttgatgaa ttatcagaat caaaatttcc acatgaaatt ccaaatacgt atgaatttgc
    10861 ccaaattcaa gttggaaaat tggctgaaat aatgtctaaa gttagaaaat caatgaaagt
    10921 ttcaaaaaat cttgctcata tggaagttct tgattcaggt gcaagtggag ctgttaattt
    10981 tgtacttggt ttttcaggca aagatgttgg cattgcatac aaagaacgtg ttgataaggg
    11041 aatttatgct gtttctgtac ggggttcacc ttcatgtaaa actcatcttg ggcgtttggt
    11101 ttcatctgtc actagtgaaa ttggtggttc aggagggggt catgataagg catgtggggc
    11161 tgttattcct aaggataaaa tcaaagaatt tttaaaaaaa atgaactcag aattaggata
    11221 attctaaaat catatcaatt tcagttgttg catctaatgg taggcttgag acaccaacag
    11281 ctgttctagt atgaacgcct ttttcagcaa atatctgata tagaaaatct gatgctccat
    11341 ctatcacttg tgcatgttca gtgaaatcag gtgatgaatt aacaaaacca gaaactctta
    11401 caatttttac aatacgatcc aaatctccaa tcttcacctt taattgtgat aaagcattga
    11461 ttgcacataa cttagcagaa gatttaccat cttcaactgt tctcggtgtt gggattttac
    11521 cagtgtatct taattttcca tttaacatgg gaatttgacc tgacacatat gctaaatcct
    11581 tgattattga cactggaata tacgaaccag caggttttgg agggtgaggc aattcaatac
    11641 ctaacgattt taatttttct tcgattatca tgattaaatt caaataattc acgcctataa
    11701 gtaatagtgt aaataattta accagatttt ctacagatat ttactcgtga tggtacctta
    11761 gcaaaacttc actgatgttc acaaggtaat ttgaatgaat ttagcaccac aagaattaga
    11821 aagaaatgct agcaaatctg cttcagatgc tataaaatat gattcacaag gtgcacgtgg
    11881 aatggctatt tctcactatg aaaaggcaat acaatcacta tctaaattga tgaagcttta
    11941 ccctgatagt aaactaaaca ctatctatac tgagagatta aaatcatatc
//
LOCUS       DASCPJ010000070         2681 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001095747, whole genome shotgun sequence.
ACCESSION   DASCPJ010000070 DASCPJ010000000
VERSION     DASCPJ010000070.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001095747"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            210..608
                     /locus_tag="QGF01_03165"
     CDS             210..608
                     /locus_tag="QGF01_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJN19929.1"
                     /translation="MNRYKKFIKKGIKEPLSTVEFHTFAPIKRVSMLSKKIIPKSDTH
                     IAIHVIDSAQKNMLDYSLLHKHNADEINLIISDNDKLKYEIQLGDEKYRVTSPTTIFI
                     PKGVRHKAKLISGKGTFVCIILSGKYTSSK"
     gene            complement(615..1724)
                     /locus_tag="QGF01_03170"
     CDS             complement(615..1724)
                     /locus_tag="QGF01_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HJN19930.1"
                     /translation="MVESNYEILNLSDDASKKEIQKAYRILVLQHHSDRGGNDEKFKR
                     IKQAYEDLQKGKKYPDTLDEKKSNAKFYSGTSEDEKLRKNLLLSNDVAKEMKTAQEWV
                     AALSRTNTTGMRLFGSNEFGKMEFERKPTKTLSMKGKFWAGNFKYDNSVMMWGSITSP
                     YFSPYEKHKTHIHLTDGNFRMIDSIQNKYDIDGGAKITVDNGDMEVGNVSGIKKRVSD
                     PQGRVGMSIMKEYFTELKALNGKLVAGNVTDTVKLDADTVIALNLVDNIKIKGREILV
                     YGSKVTHNVEFFLKNNGKIRFYDQGSGFDISDDATIQLESDRIIKIRDLKRAKMISYG
                     GKDIRYEDIVNLGTRKKSKNGTSNNFGSFKKIFGR"
     gene            1810..2397
                     /gene="dps"
                     /locus_tag="QGF01_03175"
     CDS             1810..2397
                     /gene="dps"
                     /locus_tag="QGF01_03175"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HJN19931.1"
                     /translation="MAESKKPVVVGIEILKKNGLDVDSLIKELVINASVEFTAYYYFT
                     LLRANCTGMDGEGIKGVIEDARLEDLSHFESCIERIYQLGGSLPKDATQFIKMSGCEF
                     LQLPPNPTDLTAILEKCLKAEQGAIVNWDKICNMTLGKDPATYDIAKDILAEEIEHES
                     WFLELLYSRPSGHMRRKYPGERPHTKKHSRALDSL"
     gene            complement(2432..>2681)
                     /locus_tag="QGF01_03180"
     CDS             complement(2432..>2681)
                     /locus_tag="QGF01_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19932.1"
                     /translation="FLIEDATKSYLNLKLNEAHPPNFGRIIFYVDNVDDLFAYMESDE
                     TISGLGKLESKPEDAEWGERFFHALDPDGYKLSFATPI"
BASE COUNT          860 a          484 c          417 g          920 t
ORIGIN      
        1 gtttcaaaag agccagagag cgaaccccat gccgcaaagc atgtaacgat gctagtgtat
       61 ggcaaaaaca gcaggccaat tgtcactcga aaattcctag agccaaattt tggaacaaac
      121 cactctgatg tcctatcccg aagcatggaa actgtacgtc aaagtatgtt tttaacttag
      181 attttatagt atgtaaaatg aattagatta tgaatcgcta taagaaattt ataaaaaaag
      241 gaatcaagga accactctca actgtcgaat ttcatacttt tgcgccaata aagcgtgttt
      301 caatgcttag caaaaagata attcctaaat cagatactca tattgccatt catgttattg
      361 attctgccca gaaaaatatg ctggactata gtttgttaca taaacacaat gcagatgaaa
      421 ttaatctgat tatttctgat aatgataaac tgaaatatga aattcagctt ggtgatgaaa
      481 aatacagggt aacgtcacca acgacaatat tcattccaaa gggagtacgg cataaggcaa
      541 agttgatatc tggcaaaggg acttttgtct gtattattct gtcaggaaaa tatacgtcat
      601 caaaatagat acaattatcg cccaaatatt tttttaaatg aaccaaaatt atttgatgtt
      661 ccattctttg atttttttcg agtacctagg tttacaatat cttcatatct aatgtctttg
      721 ccgccataac tgatcatttt tgcacgtttt agatcacgta tttttatgat cctatcggat
      781 tccaattgta ttgtggcatc atcacttatg tcaaagcctg aaccttgatc ataaaacctg
      841 attttgccgt tattttttaa gaagaattct acattgtgtg ttaccttact gccgtaaact
      901 agtatttctc ttcctttgat ttttatatta tcaactagat taagtgcaat cacggtatca
      961 gcatcaagtt ttacagtatc agttacattt ccagcaacaa gttttccatt cagagctttg
     1021 agttcagtga aatattcttt cataatcgac atgccaactc ttccctgtgg atcactaacc
     1081 ctctttttta taccacttac attaccaact tccatatctc cgttatcaac agtgattttt
     1141 gctccaccat caatatcata tttattttga atggaatcaa tcattctaaa atttccatct
     1201 gtaagatgta tgtgggtttt atgtttttca tatggactaa aatatggact tgtgatacta
     1261 ccccacatca ttactgaatt atcatatttg aaattaccag cccaaaattt acctttcatt
     1321 gacaatgttt ttgtgggttt tctttcaaac tccattttac caaattcatt tgaaccaaat
     1381 aatcgcatac cagtagtatt ggtacggcta agagcagcaa cccattcttg cgctgttttc
     1441 atttcttttg ctacatcatt agataataga agattctttc gtaatttttc atcttccgag
     1501 gttccagaat aaaattttgc gttactcttc ttttcatcta atgtatcagg atatttttta
     1561 cctttttgca ggtcttcgta tgcctgctta attcttttga atttttcatc attgccacct
     1621 ctatctgagt gatgttgaag taccaatatt ctgtaagctt tttgaatttc ttttttagac
     1681 gcatcatcag atagattcaa aatttcataa ttactctcta ccatacttaa catacatctg
     1741 atactttctt aaatcttaag aaattaaata caaaaatcct ttttatttag tgataattac
     1801 aatctgcata tggcagaatc aaaaaaacct gttgtagtgg gtattgagat tttaaaaaaa
     1861 aatggacttg atgtagattc tcttatcaaa gaattagtga ttaatgcatc tgttgaattt
     1921 acggcgtatt attacttcac attattacgg gcaaactgta ctggtatgga tggtgaagga
     1981 attaagggtg tgattgaaga tgcaagatta gaagatctaa gtcactttga atcatgcatt
     2041 gagagaatct atcaacttgg tggctctcta ccaaaagatg ctacacaatt cattaagatg
     2101 tctggctgtg aatttctaca actcccacca aacccaacag atctaactgc aattctagaa
     2161 aaatgtctca aagctgagca aggtgcaata gtaaactggg ataaaatatg taatatgaca
     2221 ttgggtaagg atccagcaac gtatgatatt gcaaaggata tactagctga agagattgag
     2281 catgaatcat ggtttctaga gctcttgtac tcaagaccat ctggtcatat gagaagaaaa
     2341 tatcctggtg aaagaccaca cacaaaaaaa cattctaggg cacttgattc gttataattc
     2401 taaatttttt ataaataatt agactatttt ttcaaattgg tgttgcaaat gatagtttgt
     2461 aaccatcagg atctaatgcg tgaaaaaatc gctcacccca ttctgcatcc tccggcttgg
     2521 attctagttt acctagcccc gaaattgttt cgtcactttc catgtatgca aaaagatcat
     2581 caacattatc aacgtaaaaa ataattctac caaaatttgg tggatgtgct tcattcaatt
     2641 ttagatttag atatgacttt gttgcgtcct ctatcagaaa t
//
LOCUS       DASCPJ010000071         4465 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001100599, whole genome shotgun sequence.
ACCESSION   DASCPJ010000071 DASCPJ010000000
VERSION     DASCPJ010000071.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001100599"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="QGF01_03185"
     CDS             <1..288
                     /locus_tag="QGF01_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJN19933.1"
                     /translation="SEFVERMQLPNAKMAFMSTLLGLSNAKVITEKLNMITVPTMIVW
                     GENDPVIPIEYAQSFVSGINDCRFYKMLGCGHTPYVENSEKFFQIVVDFLD"
     gene            320..619
                     /locus_tag="QGF01_03190"
     CDS             320..619
                     /locus_tag="QGF01_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HJN19934.1"
                     /translation="MVMEGKKNPYDPVELYREWLEKASEAQSNFANMNPFQANAQNPF
                     NLQNMLPNFLSWGAFKTSVGSNGRISIPEAERDALSVNEGDLVQVIVIPVNPKSK"
     gene            693..1109
                     /locus_tag="QGF01_03195"
     CDS             693..1109
                     /locus_tag="QGF01_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19935.1"
                     /translation="MSNQTDKNDVFSTVEQTVDKQIKSTEKSFPNYQTSVTSLQQEYT
                     EAFKNVVESSIALQREFANKTGINTGVPQATEDAVRQSNEQISKVQDVQNKIALATID
                     ATRQNVKTFNENAKAFADLNKSVMQSWITAFTPKFN"
     gene            complement(1132..2193)
                     /gene="phaC"
                     /locus_tag="QGF01_03200"
     CDS             complement(1132..2193)
                     /gene="phaC"
                     /locus_tag="QGF01_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HJN19936.1"
                     /translation="MTQNRFDPKIIGEFLNFGRNITEAPMKISIPHEVRIGSTEFDVV
                     YKEDKMRLLHFKPLTTKQVKTPLLIVYAIINRYHIFDIDPKKSWVKNLLEQGFDLYMI
                     DWGTPTSLDKFLGFDDYVNRYLDNCVEFICNESDVKKISMQGYCTGGTLATVYSALHP
                     EKIKNLVVTAPVIDGWKDTTVVSNLTKSFDVDKFVDTIGNMPPEFMYYCFSILKPFEQ
                     GVEKYLKFMNNINNEKFIESFLKIEKWLDETPPIPGQLFKEWIKGIYQDNLLIQNKMF
                     VGNKLVSLEKLDMPIFTQVAVGDHLVSPECSMPLHYAVSSTDKTLQLYPTGHVGMIAS
                     SFSQKKVLPELGQWLKERS"
     gene            complement(2180..2752)
                     /locus_tag="QGF01_03205"
     CDS             complement(2180..2752)
                     /locus_tag="QGF01_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HJN19937.1"
                     /translation="MQNGSNNPYDYYKKTAVFWSDMITMMSSKPTALSAVGPLRNLST
                     NMKKITAEITEANKEIVEFNNFLIEYYKQLSQTWTDAQNEVTAKTSQLPKDEEGTEAY
                     KRVWIDNFENYFTGLFDSKKFSENYNKLISKELDLMKRWNVVMDIMLKSANMPTKQEI
                     DEIYKELHSLKQKISKLESSTKKSDKNDAE"
     gene            2803..3147
                     /locus_tag="QGF01_03210"
     CDS             2803..3147
                     /locus_tag="QGF01_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HJN19938.1"
                     /translation="MKYDELCERIKKVDPKIRFAGVINSKGRLVSGGMVSSKKPLEDT
                     KKDEMLFMELALRVKMRSEFDDELGQVKFSLSFREKLIVMSFPLKNDVLLVSMDKRMS
                     FDTIPFKILKLL"
     gene            3237..3866
                     /locus_tag="QGF01_03215"
     CDS             3237..3866
                     /locus_tag="QGF01_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HJN19939.1"
                     /translation="MGQFFSWVKSNEKQILVILDNLAKKGVEVSEAVVVMLSDLDNPE
                     HHKQIHILETEADSLVREIFSELNSTFITPLDREDMQRVASKIDDTIDHMDGIASRLY
                     SYKITITPPYALDIANELVNATKEVELMTSKLQNMKNPSTMIEHCRRTSAIEHTVDDL
                     YKLAMSELFESTDAIQIIKLKDIYESMETASDRCQDVADVVEDIVLKYT"
     gene            3875..>4465
                     /locus_tag="QGF01_03220"
     CDS             3875..>4465
                     /locus_tag="QGF01_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HJN19940.1"
                     /translation="MIEVAIGAIIVALIFDFVNGFNDSANSVATVIGTRVLKPLHAVA
                     LSAAANFVGPFIFGVAVATTIAKGIVSPDEVTVYMIIGGLAGAISWSSLCTYFGLPIS
                     NSHSLIGGIMGAGIAGLGFEQLVYGGLVKVFAGIIIAPIGGIVFGMALVGIVIAIFAK
                     YRPAIVNRTFGRLSIISSAWLALTHGANDGQKTMGII"
BASE COUNT         1457 a          749 c          818 g         1441 t
ORIGIN      
        1 tctgaattcg tagaaaggat gcagttacca aatgcaaaga tggctttcat gtcaacactc
       61 ctaggtttga gtaatgccaa agtgatcact gaaaaattga acatgatcac agttcctact
      121 atgattgtat ggggtgaaaa tgacccagta atcccaattg aatatgctca atcttttgtt
      181 tctggaatca atgattgtag gttttacaag atgttaggct gtggacatac accatatgta
      241 gaaaattcag aaaaattctt ccaaattgtg gtagattttc tcgattaacg atcattttat
      301 taaggttgtt tacctatcaa tggtaatgga aggaaagaaa aacccgtatg atccagttga
      361 attgtacagg gaatggctgg aaaaagcatc agaggcacag tctaattttg caaacatgaa
      421 tccatttcag gcaaatgccc aaaatccatt caatttgcaa aacatgctgc caaactttct
      481 ctcttggggc gctttcaaga ccagtgttgg tagtaatgga agaatctcaa ttcctgaagc
      541 ggaaagagat gccttgagtg tgaatgaagg tgatttagtg caagttatcg ttattccagt
      601 taaccctaaa agcaaataat attggtaaaa aatggtaatg attggttttc attaccagta
      661 gatatatata atcatgccaa ttagaacata tcatgagtaa ccaaactgac aagaatgatg
      721 tattctcaac tgtagaacag actgtagata aacagattaa atctactgag aaatcattcc
      781 caaactatca gacatctgtt acaagcttac agcaagaata cactgaagca ttcaagaacg
      841 tcgtagaatc aagtattgct ttgcaaagag aatttgctaa caagactgga atcaacaccg
      901 gtgttccaca ggcaacagaa gacgctgtac gccaatcaaa tgaacagatt agcaaggtac
      961 aagatgttca gaacaaaata gcattagcaa caattgatgc tacccgtcag aatgtcaaga
     1021 ccttcaacga gaacgcaaag gcttttgctg atttaaacaa aagtgtaatg caatcttgga
     1081 tcaccgcatt cacacctaaa tttaactagt gtgaatgact acttttttta tttatgacct
     1141 ttctttaagc cattgaccaa gttctggtaa gacctttttt tgactgaacg aactagctat
     1201 cattccaaca tggccagtag gatacagttg taatgtcttg tcagtgcttg aaacggcata
     1261 atgtaacggc atgctacatt caggagatac tagatgatct cctaccgcaa cctgagtaaa
     1321 aataggcata tctagctttt ctaacgaaac cagtttgtta ccaacaaaca tcttattctg
     1381 aatcaaaaga ttatcctgat aaatcccctt tatccattct ttaaaaagtt ggccaggaat
     1441 aggtggtgtt tcatccaacc atttctcaat tttgagaaaa ctttcgataa acttttcatt
     1501 atttatatta ttcatgaatt tcaagtattt ctcaacacct tgctcgaatg gtttgagtat
     1561 tgagaaacaa tagtacatga attctggagg catgtttcct atagtatcta caaatttgtc
     1621 aacatcaaat gatttggtca aattactaac tactgttgta tctttccaac catcaattac
     1681 tggagcagtt acaaccagat ttttgatttt ttcagggtga agtgcagagt acacagtggc
     1741 caaagttcct ccggtacagt aaccttgcat tgatattttt ttcacatcag attcattaca
     1801 aataaactca acacaattat ccaggtaacg atttacataa tcatcaaatc ctagaaactt
     1861 atccaagcta gttggtgttc cccaatcaat catatataga tcaaatcctt gttcgagtaa
     1921 attttttacc caacttttct ttggatcaat atcaaaaata tgatatctat tgataattgc
     1981 atagacgata agtaatggtg tctttacctg ttttgttgta agtggtttaa aatgtaacaa
     2041 gcgcatttta tcttccttat acacaacatc aaattctgtt gagccaatgc gaacctcatg
     2101 tggtatagag atcttcattg gtgcctctgt tatattacga ccgaagttta gaaactcacc
     2161 aataattttt gggtcaaatc tattctgcgt catttttatc agattttttt gtggatgatt
     2221 ctaattttga aattttttgt ttcagggaat gtagctcttt gtatatttca tcaatctctt
     2281 gcttggttgg catattagcg gattttagca ttatatccat cacaacattc catcttttca
     2341 taagatctaa ttccttagaa atgagtttgt tatagttttc cgagaatttt ttagagtcaa
     2401 acaatcctgt aaaataattt tcgaaattat ctatccaaac tcttttgtag gcttctgtgc
     2461 cttcctcatc cttaggtaat tgagatgttt ttgcagttac ttcgttttgt gcatcggtcc
     2521 atgtttgaga taattgttta taatattcta ttagaaaatt attgaactcc acaatttcct
     2581 tgtttgcttc ggtaatctct gccgtaattt ttttcatatt tgtcgaaagg ttacgtagag
     2641 gaccaactgc ggaaagtgct gtaggcttgg atgacatcat cgttatcatg tcagaccaaa
     2701 acactgctgt ttttttgtag taatcatagg gattatttga gccattttgc atgcgtatta
     2761 taatatggat ctcaataaat agatcgcgga ttatagatac atatgaaata tgatgaatta
     2821 tgtgaacgta tcaaaaaggt tgatcccaag ataaggtttg caggcgtgat taattcgaaa
     2881 ggcagacttg tctcaggagg aatggtttca agtaaaaaac ccctagaaga tacaaaaaaa
     2941 gatgaaatgc tcttcatgga acttgcactt agggtcaaaa tgaggagtga atttgacgac
     3001 gaacttggac aagtaaagtt ctccttatcc tttagagaaa aattgattgt gatgagcttt
     3061 cctttgaaaa atgatgtgtt gcttgtatca atggataaga gaatgtcatt tgacacaatt
     3121 ccatttaaga ttctcaaatt actatagatg tttatttttt gattgggttt tattactata
     3181 tagtacatat agattatgta cgccatagtt aagttcaaaa ctttgctacg cagattatgg
     3241 gacaattctt ttcctgggta aaatcaaacg aaaaacaaat tttagtaatt ctagataatt
     3301 tagcaaaaaa aggagttgaa gtctctgaag cagtagtagt tatgttatcg gatcttgata
     3361 atcctgaaca tcataagcaa atccatattc ttgaaactga ggccgattct ctagtacggg
     3421 agattttttc agaattaaac agtacattca tcacaccttt agatcgtgag gacatgcaaa
     3481 gagttgcatc aaaaatagat gatacaattg atcacatgga cggaatagct tcaagacttt
     3541 atagctacaa aataaccatt acccctccat atgcattgga tatagcaaat gagcttgtaa
     3601 atgcaacaaa agaagtagaa ttgatgacat ctaaactgca aaatatgaag aatccttcta
     3661 caatgataga acattgtaga aggacaagtg caattgaaca tacggtggat gatttgtata
     3721 aactagctat gtctgaacta tttgagagta ctgacgcaat acaaatcatt aaactaaaag
     3781 atatctatga atcaatggaa accgcatcag atagatgcca agatgttgct gatgttgttg
     3841 aagatatcgt tctaaaatat acctagatga atttatgata gaagtagcaa ttggagcaat
     3901 aatcgtagca ctaatctttg attttgtaaa tgggttcaat gattctgcta attctgttgc
     3961 tacggtaatc ggaacgcgtg ttttaaaacc gcttcatgca gttgccctat ctgcggccgc
     4021 taattttgta ggaccattca tttttggtgt tgcagttgct accactattg caaaaggaat
     4081 tgtaagtccc gatgaggtta cagtttatat gattattgga ggtttggcag gcgcaatatc
     4141 ttggagttca ttatgcacat attttggatt accaatttcc aacagtcatt ccttaatcgg
     4201 cggaataatg ggagcaggta ttgcgggatt aggctttgaa caacttgttt acggtggatt
     4261 agtcaaagtt ttcgcaggaa taattatcgc accaattggt ggaatagttt ttggtatggc
     4321 tttagtagga attgtaattg caatctttgc gaaatataga ccagccatag taaacagaac
     4381 atttggcaga ttatcaatta tatcatctgc atggctcgca cttacacatg gagccaatga
     4441 tgggcaaaaa actatgggaa taatt
//
LOCUS       DASCPJ010000072         8517 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001125247, whole genome shotgun sequence.
ACCESSION   DASCPJ010000072 DASCPJ010000000
VERSION     DASCPJ010000072.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001125247"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="QGF01_03225"
     CDS             <1..269
                     /locus_tag="QGF01_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HJN19941.1"
                     /translation="PVAEPEPVAEPEPVAEPEPVAEPEPVAEPEPVAEPEIIESSNDP
                     FEGIKTDDINNFADLYNIAPPENDKEAYELAKKIRKWIADGRPK"
     gene            316..1227
                     /gene="argF"
                     /locus_tag="QGF01_03230"
     CDS             316..1227
                     /gene="argF"
                     /locus_tag="QGF01_03230"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HJN19942.1"
                     /translation="MKIKTKNLLTIDELSSKEFLGLIDFSIKLKSKKKKTKGKPLLEN
                     KTLAMIFEKPSTRTRVSFETGMLQLGGHAISLSLTDMQHSRGESIKDTAKALSRYVDA
                     IVARVYEHSFLEDLAKHATIPVINGLSNSYHPCQTLADLMTVKEYKKKFKGIKIAWVG
                     DGNNVCNSLIFGCSKTQTHLSIATPKGFEPNPAVVKKSSKHTTIDLTTDPKLAVKDAD
                     VVMTDSFVSIHDSDAKSKKFLPTYQVNKSLMDKAKNDAIFMHCLPAKRGHEVTNSVMD
                     STQSVIWDQAENRLHSQKALLASLLSV"
     gene            1265..1753
                     /locus_tag="QGF01_03235"
     CDS             1265..1753
                     /locus_tag="QGF01_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HJN19943.1"
                     /translation="MAYSKILRRIRNEKTNYNKRKRMLMGHSDFISVQISNENIQVQI
                     HQPKITGDLVISSAHSRLLTQRGWKGSRKNIPAAYLTGYYAGKKALGKGTSSAIMYSG
                     TRKYTQRMAAALKGVIDAGLQVPADEESFPIESRINGEHLKVKNDIKNLKSLIDNEVE
                     HK"
     gene            1750..2475
                     /locus_tag="QGF01_03240"
     CDS             1750..2475
                     /locus_tag="QGF01_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HJN19944.1"
                     /translation="MSQTSQAQTSKPPGQGRPGQGRPGQGRPGGRPGQGRPRRERKPI
                     VEPEWIPKTILGKKVASGEISSMEEILSQGLRIQEAGIIKKLVPDLETKVFDVGIIQK
                     MTPNGQSTSFKALVAAGNKNGWLGIGMGKSKQMRVAIEKANNAAYINVSPIKLGCGSW
                     ECRCDQKHSVPFKVKGKGGSVTIEILPGPRGLGLVAGGKIRDLLQLAGLKDAWTTAKG
                     STATMNSTSKALLNCLRQTFSQG"
     gene            2480..2947
                     /locus_tag="QGF01_03245"
     CDS             2480..2947
                     /locus_tag="QGF01_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HJN19945.1"
                     /translation="MAKAFLVVRIKGQADVPHWANTTLNLLNLEKKYRAVIIPVKENT
                     VGMLRKVQHYLSWQEIDIETTKELLDKKGRKSGYKKITGEDVSKAGFKTIDELATSLS
                     EGKISMSKIKPIKPWFALSPPRQGFKRSTKRLYGQKGVLGHNKELTTLIRRMM"
     gene            2949..3425
                     /locus_tag="QGF01_03250"
     CDS             2949..3425
                     /locus_tag="QGF01_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HJN19946.1"
                     /translation="MATRLRKTRKLRGGRHMGWGQVAQHRASGHKGGLGRSGHMKHLW
                     STVIKEDQDHFGHKSSHTPHPNITKKWASVRDLDDLYLQYGKEENGKKTLNLTLLGFD
                     KLLGGGQVKNAYTITIKQTTKSAIEKVKQSNGEIIGIIQKTKETPEEKIDSTNNDG"
     gene            3418..4863
                     /gene="secY"
                     /locus_tag="QGF01_03255"
     CDS             3418..4863
                     /gene="secY"
                     /locus_tag="QGF01_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HJN19947.1"
                     /translation="MAEGTLTNYIRRVVAKAEPYIPQVPKPKKKIPLQTKLLWCGACV
                     VIYMVMGQTPLYGATTPEFDFLAFARVIFASQQGSLVELGIGPIVTAGLLMQLLRGSD
                     ILKFDFKKPEERGIFQTATKLLTYIVILVEGGVYGIAVYGGNISDPTVMGVLIAQLIG
                     ASIIVMFMDELIQKGWGLGSGISLFICAGVAQQILWSLFSPMPAGDGGVVGVLPYVVQ
                     SAIHDATTGMGTLADTVFRSNQLPSIFGLCLTVSMLLILVYTQGMKVEIPIVSTKYRG
                     FAATYPIKLMYVSNIPVILASALTANAVFIGQMFWANFNPKNANPFLNILGTFDPTSP
                     SSPIGGIIYYITPPRGLDLVALDPMRAVLYVLFMIGIVMVFGKLWVELGGLSSKKAAQ
                     NLLDADVVIPGFRRSNKPVEALLNRYIPSVTILGSVILGLIAGVSDVLGVFGSGIGIL
                     LMVDILINYYNQLVREQVEVVMPRLGAWLGR"
     gene            4844..5431
                     /locus_tag="QGF01_03260"
     CDS             4844..5431
                     /locus_tag="QGF01_03260"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HJN19948.1"
                     /translation="MLGLEDNKKIVIVGIPGVGKTTVVNKIAEKLNQQNKSVSVHSFG
                     TVMFEEAKKSGVQSRDELRKLNVEQQKNLQKKAAETIANSQEDVIIIDTHAFISTKEG
                     FYPGLPNYLIQILKPTHFIAISAKPQEIYDRRTNDDTRNRDIISIGSIKKELDVQDAM
                     LSSCSVLSGSPMKVILNDEGKVEKTADTVINAIGL"
     gene            5428..6066
                     /locus_tag="QGF01_03265"
     CDS             5428..6066
                     /locus_tag="QGF01_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HJN19949.1"
                     /translation="MIEFNFILNLLDIIYLQGDIFGFREGPLGSDDPLIKGMLLSVFA
                     VIGLSILLNLFNAGIRKKLVDSVKLKRIMKETKGWQKERMAAFRSKDQKKIAELSKKS
                     QYMNKMQMEMMQMNMRPMMITIIPLLLIFYFLMPTLFSYTVALSPIPLNVIPVDFFQL
                     TCTVEKMAQDAAAGMGPYCNAENEIYLWAWYFLTAIAFSGIIMKVTKTSMNE"
     gene            6069..6629
                     /locus_tag="QGF01_03270"
     CDS             6069..6629
                     /locus_tag="QGF01_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN19950.1"
                     /translation="MIKSIIISGPPAIGKTTVAKAVAEEFNLTHLSGGDILKEFAQEE
                     GFESGGDDWWDTEEGMNFLNKRQADSKFDKKVDEKLKKYFLDGNVVITSYTLPWLVEG
                     GIKIWLAGTKENSAQRMTARDNLTKNEALDIVQKRYQENKKIYKALYGFEFGEDLSVF
                     DKIIETDSYDASQVIDLAKSTVRNLF"
     gene            6626..7639
                     /locus_tag="QGF01_03275"
     CDS             6626..7639
                     /locus_tag="QGF01_03275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HJN19951.1"
                     /translation="MKLKQLENMISIDDDTTDENYGTYFDKRSVEQLLKFGLILLDKP
                     PGPTSHEVVAWTKRILEIPKAGHSGTLDPQVSGVLPLGLGEGTKALGVLLFGPKEYHA
                     LGRLHSLPSKEQLDDILNQFTGDIYQRPPQRSAVSRQIRIRRIFELELLEQKERLILL
                     RVLCEAGTYIRKLLYDFGEVLGHGGTMIELRRTRVGQFDENYPLVTLHEVADAFATWK
                     EKKDDSLLLQLIHPIEYILSELKSVVIRDSAVDALCHGAQLAIPGILKISANLQQSEL
                     VGVYTQKGEIVALAQSTMSGEDIQKIPKGHAFETKRIIMEANTYPKRWKSQATIKEKL
                     QST"
     gene            7650..8096
                     /locus_tag="QGF01_03280"
     CDS             7650..8096
                     /locus_tag="QGF01_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19952.1"
                     /translation="MIPKGLLIAVLLGLISGPIAGAILFLQDDNQQLIFVEGPSVSIV
                     TEKIDFELKEEIVIKIINSGTVPLTFNDTSYGLRITGLDGVLYYTPMAAQVISELEPK
                     DEITFVWDQQKLDGSYSLEGRYKITTEGFDPENNKVKKSIVINVLK"
     gene            8127..8224
                     /locus_tag="QGF01_03285"
     tRNA            join(8127..8164,8179..8224)
                     /locus_tag="QGF01_03285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8161..8163,aa:Ser,seq:gga)
     gene            8256..>8517
                     /locus_tag="QGF01_03290"
     CDS             8256..>8517
                     /locus_tag="QGF01_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJN19953.1"
                     /translation="MKKYDIAIIGGGILGTSISYWISTLHDLNVCVIEKEEDVGQHSS
                     TRNSGVVHYPFYLNSKRKKNFSKAAFLSHKMWKALANENNVPW"
BASE COUNT         2993 a         1365 c         1729 g         2430 t
ORIGIN      
        1 aaccagtagc tgaaccagaa ccagtagctg aaccagaacc agtagctgaa ccagaaccag
       61 tagctgaacc agaaccagta gctgaaccag aaccagtagc tgaaccagaa attatagagt
      121 catctaatga tccttttgaa ggaattaaaa ctgatgacat aaacaatttt gcagatctat
      181 ataatattgc acctcctgag aatgataaag aggcatatga attggcaaaa aaaatacgta
      241 agtggattgc tgatggacga ccaaaataaa attacaaaac ctaaaaagta atagaattat
      301 acggaaattt agaaaatgaa aattaaaaca aagaacctct taacaattga tgagctttct
      361 agcaaagaat ttttgggact gatcgatttt tccataaaac taaagagtaa aaagaaaaaa
      421 acaaagggaa aaccattgct agaaaataaa accttagcca tgatatttga aaaaccatca
      481 acacgaacta gggtaagttt tgaaactgga atgttacagt taggaggaca tgctataagc
      541 ttgtcattaa ctgacatgca acattcacgt ggggaatcaa ttaaagatac agcaaaggct
      601 ctgtctcgtt atgttgatgc aattgtagca cgggtgtatg aacatagttt tctcgaggat
      661 cttgcaaaac atgctacaat tccagtcatt aacggattat ccaattcata tcatccatgt
      721 cagactttag cagatctgat gactgtaaaa gaatataaga aaaaattcaa gggtatcaag
      781 atagcttggg ttggtgatgg aaataatgtg tgtaattcat taatttttgg ttgcagtaaa
      841 acacaaactc atctatccat agcaacccca aagggttttg aaccaaaccc agctgttgtg
      901 aagaagtctt ccaaacatac tactattgac ctaacaactg atccaaaact tgctgtcaaa
      961 gatgcagatg tggtaatgac cgattcattc gtatccatac atgattctga tgcaaaaagc
     1021 aagaaattct tgccaactta ccaagtgaac aaatcattga tggataaagc caaaaatgat
     1081 gcaattttta tgcattgtct tccagcaaaa cgcggtcatg aggtaactaa ttcggttatg
     1141 gatagcacac aatcagttat ctgggatcaa gcagaaaata gactacactc acaaaaggct
     1201 ttgcttgcat cattgctcag cgtctaacgt ttataaaagc agattcaaat tttttcgtat
     1261 agatatggca tattcaaaaa tattacgtag aataagaaat gaaaaaacga attataataa
     1321 aaggaagcgg atgctaatgg gtcatagtga ttttatttct gtacaaattt caaatgaaaa
     1381 tatacaagtt caaattcatc aacctaaaat tactggtgat ttggtaattt catctgcaca
     1441 ttccagattg ttaactcaaa gaggttggaa aggatctcgt aaaaatatac cagctgctta
     1501 cttaactggt tattatgctg gtaaaaaggc attagggaaa ggaacaagtt cagctattat
     1561 gtatagtggt acaagaaaat atactcagcg tatggccgcg gcactaaagg gtgttataga
     1621 tgcaggactt caagtaccag cagatgaaga atcatttcct atagaatcta gaataaacgg
     1681 tgagcatctt aaagtaaaaa atgatattaa aaatctgaaa tctttgatag ataatgaggt
     1741 agaacataaa tgagtcaaac ttcacaagca caaacttcta aaccaccagg tcaaggacgc
     1801 ccaggtcaag gacgcccagg tcaaggacgc ccgggaggac gcccaggtca aggacgccca
     1861 cgtcgtgaaa gaaagccaat tgtagaacca gaatggatac caaaaactat tttaggaaaa
     1921 aaagttgcgt ctggtgaaat cagttctatg gaagaaattt tatctcaagg gttaagaatt
     1981 caagaagctg gaataataaa aaaattagtt cctgatttag aaacaaaagt atttgatgtt
     2041 ggtataattc aaaaaatgac acccaatgga caatctacta gttttaaagc attagttgct
     2101 gcaggaaata aaaatggatg gttaggtatt ggtatgggaa aatcaaaaca aatgagagtt
     2161 gctattgaaa aagcaaataa tgctgcgtac attaacgtta gtcctataaa attaggatgt
     2221 ggtagttggg aatgtagatg tgatcaaaaa cattcagtac cattcaaagt aaaaggaaag
     2281 ggtggaagtg taacaattga aattcttcca ggtccgcgtg gacttgggtt agttgcaggt
     2341 ggaaagatta gagatttact tcaactcgct ggcttgaaag atgcatggac tactgcaaaa
     2401 ggttcaactg ctacaatgaa ttctacatca aaagcactct taaactgttt gaggcaaaca
     2461 tttagtcagg gttgattata tggcaaaagc atttttggtt gttagaataa aaggacaagc
     2521 cgatgttcca cactgggcta acactacatt aaatctatta aacttggaaa agaaatatcg
     2581 tgctgttata atacctgtca aagaaaatac tgtaggaatg ttaagaaaag tacaacatta
     2641 tctctcttgg caagaaattg atattgaaac aactaaagaa ttattggata aaaaaggccg
     2701 aaagtcaggc tacaaaaaaa ttactggtga ggatgtatcc aaggcaggtt ttaagacaat
     2761 tgatgaatta gcaacatctt tgtcagaagg gaaaattagt atgtctaaaa taaaaccaat
     2821 aaaaccatgg tttgccttgt ctccacctag acaagggttt aaacgaagta caaaacgctt
     2881 gtatggtcag aagggtgttc ttggacataa taaggaactg accactttga ttaggagaat
     2941 gatgtaaaat ggcaacaaga ttacgaaaaa ctagaaaact tagaggagga aggcacatgg
     3001 gatggggtca agttgcgcag catcgtgcta gtgggcataa aggtggtctt ggaagatctg
     3061 gtcacatgaa acatctgtgg agtacagtta tcaaagaaga tcaagatcat tttggtcata
     3121 aatcatctca tacaccacat ccaaatatta caaaaaaatg ggcaagtgtc agggatcttg
     3181 atgatttgta tttacagtat ggaaaagaag aaaatggtaa aaaaacctta aacttgacat
     3241 tattaggttt tgataaactt ctaggaggtg ggcaagtaaa gaatgcgtat acgattacta
     3301 ttaaacagac aactaaatct gctattgaaa aagtaaaaca gtctaatggt gaaataattg
     3361 gaattataca aaaaacaaaa gaaacaccag aagaaaaaat agattctaca aataacgatg
     3421 gctgaaggaa ctctaactaa ttatattcga cgtgttgttg caaaggcgga accttacatt
     3481 cctcaggttc caaaaccgaa aaaaaagata cctcttcaaa caaaattact gtggtgtggg
     3541 gcttgtgttg ttatctatat ggtaatggga caaactcctc tttatggtgc tacaactcct
     3601 gaatttgatt ttcttgcatt cgcacgtgtt atttttgcct cacaacaggg ctctctggtt
     3661 gaacttggaa ttggaccaat tgtaactgct ggtttactta tgcaattatt gagaggttca
     3721 gacattctaa aatttgactt taaaaaacca gaagagcgtg gtatattcca gactgccact
     3781 aaacttctta cttacatagt catacttgtc gaaggaggtg tctatggcat agcagtttac
     3841 ggcggaaaca tttcggatcc tacagttatg ggtgttttga tagcacaatt gattggagca
     3901 tcaattattg tcatgtttat ggatgagcta attcaaaagg gatggggttt aggatcagga
     3961 attagtttat tcatctgtgc tggtgttgct caacaaattc tttggagtct atttagtccg
     4021 atgcctgctg gtgatggtgg agttgttggc gtactgccgt atgttgttca atcagctata
     4081 catgatgcaa caacaggcat gggtacactt gctgatactg tctttaggtc aaaccaactg
     4141 cctagtatat ttggtctttg tcttacagtt tccatgttac tgattctagt ttatactcaa
     4201 ggtatgaaag ttgaaatccc aattgtatcc acaaaataca gaggatttgc agcaacctat
     4261 cctatcaaat taatgtatgt ttcaaacatc cctgtaattt tagcatcagc tctaactgca
     4321 aatgcagtat tcataggtca gatgttttgg gctaatttca atcctaagaa cgctaatcca
     4381 tttttgaata tacttggaac ctttgatcct acaagcccgt catctcctat tggaggtatc
     4441 atatactata tcactccacc aagaggattg gatctagttg ctttagatcc tatgcgtgct
     4501 gttttgtatg tattatttat gattggtata gttatggtgt ttggaaaatt atgggtggaa
     4561 ttgggaggac tttcatcaaa aaaggctgca caaaatcttt tggatgcaga tgtggtgatt
     4621 ccaggattta ggagatcaaa caaaccagtc gaagctttat tgaatagata cattccatcc
     4681 gtaacaatcc ttggttctgt aatattaggt ctgatagctg gagtttctga tgtactagga
     4741 gtttttggat ctggaattgg cattttgttg atggtagata ttttgataaa ttattataac
     4801 cagcttgtaa gagagcaagt ggaagtggtt atgccgcgac ttggtgcttg gcttggaaga
     4861 taataaaaaa attgtaatag ttgggatccc aggagttggt aaaaccactg tagtgaataa
     4921 gattgctgaa aaattaaatc aacaaaacaa aagtgttagt gtgcatagtt ttggaactgt
     4981 gatgtttgaa gaagccaaga aatccggtgt tcaaagtaga gatgaattac gaaaactgaa
     5041 tgtggagcaa caaaaaaacc ttcaaaaaaa ggcagcagaa acaattgcta actcccaaga
     5101 ggatgttata attattgaca ctcacgcctt catctcaacc aaagagggat tttatccagg
     5161 attaccaaat tatctaatcc agattttaaa accaactcac tttattgcca tatcagcaaa
     5221 accacaagag atctatgatc gtagaacaaa tgatgatact cgtaacagag atatcatatc
     5281 tattggttca ataaagaagg aattagacgt tcaagatgcc atgctttcat cttgttcagt
     5341 tttatcaggt tcacctatga aagtgatttt aaatgatgag ggaaaggttg aaaaaacagc
     5401 tgatactgta ataaatgcta taggactatg attgaattta acttcatttt gaatttgttg
     5461 gatatcatct acttacaggg tgatattttt ggattccgtg aaggtccact tggaagtgat
     5521 gatccactga taaaaggaat gctgctttct gtattcgcag taattggttt atctattctt
     5581 ttgaatttgt ttaatgctgg aattagaaaa aaactagttg attctgtaaa actaaaacga
     5641 ataatgaagg aaacaaaagg ttggcaaaaa gaaagaatgg ctgcatttag atcaaaggat
     5701 cagaaaaaga ttgctgaatt aagcaaaaag tctcagtata tgaataaaat gcagatggaa
     5761 atgatgcaga tgaatatgcg tccaatgatg attaccataa ttccactatt actgattttc
     5821 tactttctaa tgcctacttt attttcatat acagtagcac tctccccgat tcctctcaac
     5881 gtaatacctg ttgatttctt ccaacttact tgtacagttg aaaaaatggc tcaagacgca
     5941 gctgcaggta tgggaccata ctgcaatgca gaaaatgaga tctatttgtg ggcatggtat
     6001 tttcttactg ctattgcatt tagtggtatt atcatgaagg ttactaagac ttccatgaat
     6061 gaatagagtt gataaaatcc ataattatct ctggaccacc agccatcgga aaaacaaccg
     6121 tggctaaagc cgttgcagaa gagttcaatc ttacacattt gagtggtggt gatattctca
     6181 aggaatttgc acaagaagaa gggtttgaaa gtggaggaga tgattggtgg gatacagaag
     6241 aagggatgaa ttttttgaat aagcgccaag cagattccaa atttgataaa aaggtagatg
     6301 aaaaactcaa aaaatatttc ctcgacggaa atgttgtcat caccagttat actttaccat
     6361 ggcttgttga aggtgggatc aagatttggt tggcaggaac caaagaaaac agtgcacaaa
     6421 gaatgactgc aagggacaat ttgacaaaaa acgaggcatt agatatagta caaaaacgct
     6481 atcaggaaaa taaaaaaatt tacaaggcat tgtatggatt tgaatttgga gaagaccttt
     6541 ccgtttttga taaaataatt gagactgaca gttatgatgc atctcaagtt attgatcttg
     6601 ccaaatctac tgtgaggaat ctcttttgaa actaaagcag ctcgagaaca tgatcagtat
     6661 tgatgatgat actactgatg aaaattatgg tacctatttt gacaagcgtt cagttgaaca
     6721 attactgaag tttggcctca tacttttaga caagccgcca ggcccaacta gtcatgaagt
     6781 agtggcatgg accaagcgaa tactggaaat tcccaaggct gggcatagtg gaaccttgga
     6841 tccacaagta tctggagtac ttccattagg acttggagaa ggtactaagg ctcttggtgt
     6901 tttactcttt ggaccaaagg agtatcatgc actaggcaga ttgcattcat taccatcaaa
     6961 agaacagctt gacgatattt tgaaccaatt tactggagac atttaccaaa gaccaccaca
     7021 acgttcagca gtttccagac aaattcgtat tcgacgtatt tttgaattag aattattgga
     7081 acaaaaggaa agactgattc tgcttcgtgt tttatgcgaa gctggaacat atattcgaaa
     7141 attactatat gattttggtg aagtcttagg acatggaggt accatgatcg agttaagacg
     7201 tacacgtgta ggtcaattcg atgagaatta tcctttggtt actcttcatg aggttgcaga
     7261 tgcttttgca acttggaagg agaaaaaaga tgattcgtta ttattgcaat taattcatcc
     7321 aatagaatat attttgagtg aattaaagtc ggttgttata cgtgattctg ctgttgatgc
     7381 gttatgtcac ggtgcacaac ttgccatacc tggcatacta aaaatatctg cgaatttaca
     7441 acaaagtgaa cttgttggag tttatacaca aaagggtgag attgtagcat tagcacaatc
     7501 tacaatgtct ggtgaagaca tacaaaaaat tccaaagggt catgcatttg aaacaaaacg
     7561 aatcattatg gaagccaata cttatcctaa acgctggaaa tcacaagcaa ccatcaaaga
     7621 aaaacttcaa agtacttgaa agaataaaat tgattcctaa gggtcttctc atagcagttt
     7681 tgcttggttt aatttctgga cccatagctg gagcaatctt attcctacaa gatgataatc
     7741 aacaattgat ttttgttgaa ggtccatcgg tttctattgt aacagaaaaa attgattttg
     7801 aattaaaaga ggaaattgta attaaaatta ttaattccgg tactgttcca ttgacattta
     7861 acgatacatc atatggatta agaattacag gattggatgg tgtattatac tacacaccaa
     7921 tggcagcaca agttatatct gaacttgaac caaaagatga gataacattt gtctgggatc
     7981 agcaaaaatt agacggatcc tattcgctag agggcagata taaaatcaca acagaaggct
     8041 ttgaccctga aaataataaa gtgaaaaaat ccatcgtgat aaacgttcta aagtaagata
     8101 ttcggaaatt tattggcgaa tagaatgccg gggtcgccta gcctggtagg gcgcgcgcct
     8161 ggaaataatt taccataaag cgcgttctcg caagggatcg agagttcaaa tctctctccc
     8221 ggcgccttta cataataaaa agtggtattg accatatgaa aaaatatgac attgcgatta
     8281 ttggcggagg aatattagga acctcaatct catactggat ttccacatta catgatctaa
     8341 atgtatgtgt aattgaaaag gaagaagatg tagggcagca ttcaagcact cgcaatagcg
     8401 gagtggttca ttatccattt tacctgaatt ccaaaaggaa gaagaacttt tccaaagctg
     8461 catttctttc ccataaaatg tggaaagcac tagccaatga aaataacgta ccatggg
//
LOCUS       DASCPJ010000073         3879 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001133277, whole genome shotgun sequence.
ACCESSION   DASCPJ010000073 DASCPJ010000000
VERSION     DASCPJ010000073.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001133277"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="QGF01_03295"
     CDS             complement(<1..1367)
                     /locus_tag="QGF01_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HJN19954.1"
                     /translation="MKIRKRQIHFFILLFVVSSLLFHPPFDFEANATADWYNDSWEYR
                     KKITTSLDTVISSDLTDFPYLVSFTDSDLTKTTESDGTDIVFTASDGTTELAYEIERF
                     DQSTGEVIAWVKIPTVSASENTDIYIYYKGTASSSSSSVWDSNYKQVYHLNQSSTGTE
                     NEFVDASGTGNHGTGGGEGDKTTDTDRTPTRVEGKIGYGQSFDGPTQRGINQEGTGDF
                     IWIDSVNGWTKNMDVTAEFWINVDSDHTDVNMMDVFGYRAGGQNNNLGLFKVDSLNLH
                     VRNGALHDSTVDAATSDWAYVVVVYDSGAPKIYLDGVCVSCTSTETRTGVRVDGNFVL
                     GGDIDSTTRVNNELVGDMDEFRISDTVRSADWVAASYCNQNGSNDCQTVGPEEPLTVT
                     ITTSNGNCGDTISSSPGSSATFSYTATFSTSTSDFIIDDITVTGTADDGSPVASNFAG
                     SGTAYT"
     gene            complement(1409..2548)
                     /locus_tag="QGF01_03300"
     CDS             complement(1409..2548)
                     /locus_tag="QGF01_03300"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HJN19955.1"
                     /translation="MKNRLGIIGGGQLGMMITEAAQNLTDEISEITVLDPTENCPASQ
                     SGAKQIVGDFKDEIAILKLAEQSDIITYEIESGNTDVLSKLKAKIEPSPSTLGTIQDK
                     LSQKTFLSENELPVSEFYEITSLDDLREKINELGLPVLLKSRRDAYDGRGNFKITSSD
                     EIEKAYQHFNGKSLMVEKFVNFKMEVSVIAARNTKGEIATYPLVENIHENNILKITIA
                     PARVSEDVIINAGEIAKKTMQVLNGAGVFGIEMFVDQDDKILINEIAPRVHNSGHHTL
                     QSCKTSQFEQHLRAILGLKLGSTELVHKTVMCNILGPDGFEGKYKQVELEKDGAYLKM
                     YGKEISKPQRKLGHFNVVDLSDSKDVSELLQMADEIKNTVTVSPA"
     gene            2623..3189
                     /gene="purE"
                     /locus_tag="QGF01_03305"
     CDS             2623..3189
                     /gene="purE"
                     /locus_tag="QGF01_03305"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HJN19956.1"
                     /translation="MKKSLIGIIMGSSSDSRIMHAAGEILDDFGITHEDQIISAHRTP
                     TRLDEYAKHAEKSGFKVIIAGAGGAAHLPGMIASHTVLPVIGVPIMVYNDKQQKKSEQ
                     HKFSAFGGLDSLLSISEMPSGSPVVTVGVNKAGNAAIYAMKILGNEFPKIRKMLKSHK
                     DTQHKSVLKESQELKKMGLKKFVKKKFK"
     gene            complement(3336..>3879)
                     /locus_tag="QGF01_03310"
     CDS             complement(3336..>3879)
                     /locus_tag="QGF01_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HJN19957.1"
                     /translation="DGGFTVADGISVKTPGKQTFEIIKNKIDEIVLVDDDEIINAMFL
                     LMERSKSVVEPAGAAGLAYLISKKPSPGKKVVPILCGGNIDMYLLGQIVAKGLSTMGR
                     MMKIFILLKDKPGALKEVVDELASLNANIVEVVHDRLSSSVHAGTTGVTLSLETEDQN
                     HMDKLIEHLKEKNIEFKVLT"
BASE COUNT         1201 a          782 c          632 g         1264 t
ORIGIN      
        1 gtgtatgccg tgccagatcc cgcaaagttg ctggctactg gtgaaccatc atctgcagtg
       61 ccagttactg tgatatcatc aattataaaa tcactggtag atgtactaaa ggtagcagtg
      121 taactaaatg tagcagaaga acctggacta gaggaaatcg tatcaccaca atttccatta
      181 gacgttgtga ttgttacagt taatggttct tctggtccta ccgtttgaca atcatttgag
      241 ccattttgat tacaatatga tgctgcaacc caatcagcag aacgaacagt atctgaaatt
      301 ctaaattcat ccatatcccc tactaactca ttgtttacac gcgttgtact atcaatatct
      361 ccacctaaaa caaaattacc atctactctt actccagtcc ttgtctcagt actggtacaa
      421 ctgacacaaa caccgtctag gtaaatttta ggtgcacccg aatcataaac tactacaaca
      481 tatgcccaat ctgatgttgc ggcatctaca gttgaatcat gtaatgcacc atttctaaca
      541 tgtagattaa gactatcaac tttgaacaat cccaagttgt tattttgccc tcctgctcga
      601 taaccaaaaa catccatcat gtttacatca gtatgatcac tgtctacatt tatccaaaat
      661 tccgcagtca catccatatt cttggtccaa ccatttacag aatctatcca aatgaaatct
      721 cctgtacctt cttgatttat tccacgttgt gttggaccat caaagctttg tccatatcca
      781 atttttcctt ctactctagt aggtgttcta tctgtatcag ttgttttatc accttctcca
      841 ccacccgttc cgtgatttcc agtaccagaa gcatccacaa actcattttc agttcctgtt
      901 gaagattggt taaggtggta aacttgtttg taattactat cccacacact agacgagcta
      961 gatgatgcag tacccttgta gtaaatgtaa atatcggtat tttcagaggc agaaactgtt
     1021 ggaatcttta cccatgcaat aacttcccca gtagactgat caaatctttc aatttcatat
     1081 gccagctctg tcgtaccatc acttgcagtg aaaacaatat ctgttccatc actctctgtc
     1141 gttttagtca aatctgaatc agtgaatgaa actaaatatg gaaaatctgt taaatcagaa
     1201 ctaatcaccg tatctaggga tgttgtgatt ttttttctgt attcccatga atcattgtac
     1261 caatcagctg tagcatttgc ctcaaagtca aacggaggat ggaaaagaag cgaggatact
     1321 acgaataata agatgaaaaa gtgaatctga cgctttctaa ttttcataga tcagttttcc
     1381 agcaaaacca agttaaaaat ctgttttatt atgcaggtga aactgttaca gtatttttta
     1441 tctcatcagc catttgaagt aattcagata catccttaga gtcactcaaa tctacaacat
     1501 taaagtgtcc aagcttgcgc tgcggctttg aaatctcctt tccgtacatc ttaagatacg
     1561 caccatcctt ttctaactca acttgcttgt actttccttc aaatccatca ggacctaaaa
     1621 tattacacat cacagtttta tgaaccaatt cagtgcttcc aagctttaac ccaagtattg
     1681 cacgaagatg ctgttcaaac tgggatgttt tacatgattg taaggtatga tgtccagaat
     1741 tatgtacacg tggtgctatc tcattgatga gaatcttatc atcttgatca acaaacatct
     1801 caattccaaa aacaccagca ccattcaata cctgcattgt cttttttgca atttcacctg
     1861 catttattat gacatcttca gagacgcgtg caggtgcaat tgtaatcttt aaaatattat
     1921 tttcatgaat attttctact agaggatagg tggcaatctc accctttgtg tttctggctg
     1981 ctattacaga aacctccatc ttaaaattga caaacttttc aaccatcaga ctttttccat
     2041 taaagtgctg atatgccttt tcaatctcat ctgatgatgt gatcttaaaa tttcctctgc
     2101 catcatatgc atctcttctt gattttagta aaacgggtag tccaagttcg ttaatttttt
     2161 cacgtaaatc atccaatgat gtaatctcat aaaattctga aacaggtaat tcattctctg
     2221 aaagaaatgt tttttgtgaa agcttgtctt gtatggttcc taaggttgat ggtgatggtt
     2281 caatctttgc ctttagtttg cttaacacat cagtatttcc agattctatt tcgtatgtaa
     2341 tgatgtcaga ctgctctgca agttttagga ttgcaatctc atccttaaag tcaccaacga
     2401 tttgttttgc accagattgt gaagctggac agttttctgt tgggtcaagt actgtgattt
     2461 ctgatatttc atcagtcaaa ttttgggcag cctctgttat catcattcca agctgacctc
     2521 ctcctatgat tccaagccta tttttcataa ttatcctaga aataaactag ataaaaacca
     2581 gtgcgacgtt acattaaaaa cagctgtatg tatagtacta atatgaaaaa atctcttata
     2641 ggaattataa tgggttctag ttccgacagc agaataatgc atgctgctgg agaaattctt
     2701 gatgattttg gaattactca tgaagatcag ataatctcag cacatcgaac acctacaaga
     2761 ttagatgaat atgcaaaaca cgcagaaaaa agcgggttta aggtgattat agctggtgct
     2821 ggaggtgctg cacatcttcc tggaatgatt gcgtcacata cagtactacc agtaattgga
     2881 gtaccaatca tggtttacaa tgacaaacag caaaaaaaat ctgaacaaca taaattttct
     2941 gcgttcggag gtcttgattc actactctca atctcagaaa tgccatctgg ttctcctgtt
     3001 gtaactgttg gtgtgaataa agctggaaat gcagccattt atgcgatgaa aatattgggt
     3061 aacgaatttc ctaaaataag aaaaatgctc aaatctcaca aggatacgca gcataaatct
     3121 gtcctaaaag agtctcaaga actaaaaaaa atgggtttga agaaatttgt taagaaaaaa
     3181 ttcaagtaga tatggtcttt ttatctaaga gggtctattc atgagaaaac acgagataaa
     3241 caaaagaaaa aaggctagtc agaagcgtaa gagttttggt aaaaaggaag aaaaaaaagt
     3301 caggcgtaaa attagaggta ggttttagtt aatttttatg ttaaaacttt gaattctatg
     3361 tttttctcct tcaaatgttc tatcagttta tccatatgat tttgatcttc tgtctctaaa
     3421 ctgagtgtga caccagttgt accagcgtgc acacttgagc ttaatctgtc atgaacaact
     3481 tcaacaatgt ttgcgttgag tgatgccaat tcgtcaacaa cttcttttaa cgctccaggt
     3541 ttatctttaa gcaagatgaa aattttcatc attctgccca ttgttgaaag acccttcgca
     3601 actatttgac caagtaagta catgtctata tttccaccac ataagattgg aacaactttt
     3661 tttccaggtg atggcttttt gcttattaaa tatgcaagac ctgcagcacc tgcaggttcc
     3721 acgactgatt ttgatctttc catcaaaaga aacatagcat taataatttc atcatcatct
     3781 acaagcacaa tttcatcaat cttattttta attatttcaa atgtttgttt accaggagtt
     3841 tttaccgata taccatcagc gactgtaaat ccaccgtcg
//
LOCUS       DASCPJ010000074         2694 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001154576, whole genome shotgun sequence.
ACCESSION   DASCPJ010000074 DASCPJ010000000
VERSION     DASCPJ010000074.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001154576"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            47..169
                     /locus_tag="QGF01_03315"
                     /pseudo
     CDS             47..169
                     /locus_tag="QGF01_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997661.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(477..950)
                     /locus_tag="QGF01_03320"
     CDS             complement(477..950)
                     /locus_tag="QGF01_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19958.1"
                     /translation="MNNLKLVFSSYSYAILGIVVFVSMFIPISLLSEFIFVEPYLVLH
                     VTEYSAFSFTLVVIVSIISAIVIPMNVYRIKVLRKNTSKIGGSMIGTIIGASAGICSC
                     GPIGFSVISTFGTVAGTATAFLTDYEIPLRLGSIGLLCIVYYTTSRSISTECKVS"
     gene            complement(952..1620)
                     /locus_tag="QGF01_03325"
     CDS             complement(952..1620)
                     /locus_tag="QGF01_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HJN19959.1"
                     /translation="MNKKLAIMGAAIVMVIVVVIMGSTYYSTLMQMQEYQPRMSSASV
                     DLTNGSPMLGSPDAPITIIEFGDYQCEMCYHWFHTTRADVINTYVDTGKANLVFLDLA
                     FLGRDSHPAAQATYCADDQGKYWEYHTTLYNFQEGVDDGWASKDRLVAFAFNLEMDMD
                     EFNDCMDTSKHANRVNANYDQAVKQGAQATPSFIIVSSDGTTKKISGAQPYSVFSRTL
                     EAML"
     gene            complement(1666..1839)
                     /locus_tag="QGF01_03330"
     CDS             complement(1666..1839)
                     /locus_tag="QGF01_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19960.1"
                     /translation="MSGSNNSFSKFVCTECGASFLTNRGLDVHFEKQHGIYCETCPID
                     IITKKIVSIFKQK"
     gene            complement(1911..2279)
                     /locus_tag="QGF01_03335"
     CDS             complement(1911..2279)
                     /locus_tag="QGF01_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2-type zinc finger protein"
                     /protein_id="HJN19961.1"
                     /translation="MITVNCHEVEPILHELMIYVADQVAAVPFIKFHKFILTPIMDDE
                     SVNGDDVISSIKEFLDSIGEKHNYAVISNEDTITIKSIHGKQIEREKQPQSQLFSCTH
                     CGYVTQYEVEHNNHVKIHYL"
     gene            2394..2694
                     /locus_tag="QGF01_03340"
                     /pseudo
     CDS             2394..2694
                     /locus_tag="QGF01_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965297.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          873 a          498 c          476 g          847 t
ORIGIN      
        1 ggtagccaaa gcagacttcc gatgattaga gtgatgactt ttttattcag tatccattgc
       61 cgcagtagta tcaaaacctg atgaaaaatg gggagaagta ccttgtgcat ttattgagcc
      121 tgttaaagac aaacctgtca ctgaaaaaga actgattgat ttttgtaaaa aatccaatag
      181 cgaaaattac caaaagtgaa aaatacaaaa ctctcattct aatcagatcg tatactttta
      241 cataaatttg atcgtgtgtt ttggaaagga taattgaata atgatgtatg aaaatctaac
      301 ggctactcaa tatgtcaatt agaactactc ttcttcttcg tcaggatctg attctcttcg
      361 gctaggtcta attggaatgt agatttttga cattaacaca taccaaaagt agattcaatg
      421 aatatgatga tgggttgcga aacattgtct ctttggttag attgaatgaa tcaacgtcaa
      481 ctgaccttgc attccgtgga aattgatctt gacgtggtgt aatagacaat gcacaacagt
      541 ccgatggatc ctagacggag tgggatttca tagtccgtca aaaacgcggt tgctgttcca
      601 gctacggttc caaatgttga gattacagaa aatccgattg gaccacagct acaaattcca
      661 gccgaggcac caattattgt tccaatcata cttccgccaa tcttggacgt gttttttcgt
      721 aataccttaa tcctgtaaac gttcattggt atgacaattg cagatattat cgaaactata
      781 accaccagcg taaaactgaa cgcactatac tctgttacat gaagtacaag atacggttca
      841 acaaaaatga attcggaaag tagtgatatt ggtataaaca tactaacaaa aactactatt
      901 ccaagaatag catacgaata acttgaaaat acaagtttaa ggttattcaa tttagagcat
      961 tgcttctagc gttcttgaaa aaactgagta tggctgtgca ccagaaatct tcttggtagt
     1021 tccatcagat gaaactatga tgaatgatgg cgttgcttgt gctccctgct tgacagcttg
     1081 gtcatagttt gcattaacac ggttggcatg ctttgacgta tccatacaat cattaaactc
     1141 gtccatatcc atttcaagat tgaatgcaaa tgcaactagt ctgtctttgc tagcccatcc
     1201 atcgtcaaca ccttcctgga agttgtaaag tgttgtatga tattcccagt acttgccttg
     1261 atcatccgca cagtaagttg cttgtgcagc aggatgagag tcacgtccca aaaatgctag
     1321 atctaaaaat actagatttg cttttccagt atccacatag gtgttaatca catcagcacg
     1381 tgttgtatga aaccagtggt agcacatttc acactgataa tcgccaaatt ctataattgt
     1441 aataggtgca tccggtgaac caagcatagg agaaccgttt gtaagatcta ccgatgcaga
     1501 tgacattctt ggttggtatt cttgcatttg cataagtgta gaataatacg tagaacccat
     1561 aataactaca acaataacca taacaatagc tgcgcccata atagcaagtt tcttgttcat
     1621 ctgattcctg cttttacatg agaattaaaa atctatcttg cagaactatt tttgcttgaa
     1681 gatacttaca attttcttgg ttattatgtc aattgggcat gtctcacagt agatcccgtg
     1741 ctgcttttca aaatgtacat caagaccacg attagtcaaa aatgaggcac cacattcagt
     1801 acaaacaaac tttgagaatg aattgtttga accactcatt atttatgata ttttttttat
     1861 gaatataaac aatttatttg atctcaattt tgaaaatcat acaaatttta ctatagataa
     1921 tgaattttca catggttatt gtgttcaacc tcatattgag tgacataccc acagtgtgta
     1981 caggaaaata attgactctg tggttgtttc tcacgttcta tctgtttacc atgaattgat
     2041 ttgatcgtaa tggtgtcttc atttgatatt acagcatagt tgtgtttttc accaatcgaa
     2101 tccaaaaatt ccttgattga tgaaataaca tcatcaccat tcactgattc atcatccata
     2161 attggtgtca ggataaactt gtgaaatttt atgaaaggaa ctgcagctac ctgatctgca
     2221 acataaatca tcaattcatg aaggataggt tccacttcat ggcagttgac tgttatcatg
     2281 ccaatttaat acaaaagaca aatttaatct taacattttc tgaattctta ccaacttccg
     2341 gtcaaagttt ttccttccaa agaagatatc tctaagaatt ctaaaagaat tacatggata
     2401 gttctgatat aagaaaaggg attcagagca gatgagaaga atatctttca acgcatgcaa
     2461 atcttttcac atcagactcg ctcgatggct ctagtccacc atcagttttt gttggttcat
     2521 acggatatcc aaaagttagc gtaggaccaa tggttccacc tatgcatggt gatacaactt
     2581 tacttgacag tccagaactt tgggttggaa aaagtttaga agagattgta aattttcgac
     2641 taagtctgat tagaggtatc aaaaacatac ctgtcaacga acctaacgga agat
//
LOCUS       DASCPJ010000075        18243 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001161950, whole genome shotgun sequence.
ACCESSION   DASCPJ010000075 DASCPJ010000000
VERSION     DASCPJ010000075.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 18243)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18243)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18243
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001161950"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(45..509)
                     /locus_tag="QGF01_03345"
     CDS             complement(45..509)
                     /locus_tag="QGF01_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HJN19962.1"
                     /translation="MSQESERVYTVNLGKVLLTPNNQRAKRAINMVKEFARRHMKMEQ
                     IKIEEDLSHLLWSRGIKHPPRKVRVRMTKTDEGYILISKYEGEEHQEKKKDEKIKPVP
                     EPVIESEVVESEPVEPAAKAKPAAKAKPAAKAKPAAKAKPAAKAKPAAKAKK"
     gene            complement(509..667)
                     /locus_tag="QGF01_03350"
     CDS             complement(509..667)
                     /locus_tag="QGF01_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HJN19963.1"
                     /translation="MAARKHSGRKIRLMKKIKQTSSVPAWIMLRTNKKVRTNPKRRAW
                     RQTDVEVG"
     gene            complement(725..1285)
                     /locus_tag="QGF01_03355"
     CDS             complement(725..1285)
                     /locus_tag="QGF01_03355"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HJN19964.1"
                     /translation="MDIDGIRTALTSKISPVQFPADGNILAAVLIIIYGHDPIILMTK
                     KAKTLKQHGGEISFPGGKWQSSDHDLLETAIRETNEELSLSIDRNQILGQLDNVTTLN
                     SKYTIAPFISIIDKIPNLKPNSEVESVLHIPLFSFLETISNDTNHEHNSIKEMYTLTF
                     EKHIVWGASARMLRQLNILLNRKKLL"
     gene            1359..2090
                     /locus_tag="QGF01_03360"
     CDS             1359..2090
                     /locus_tag="QGF01_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methenyltetrahydrofolate synthetase"
                     /protein_id="HJN19965.1"
                     /translation="MTIMYEINPPRISAEQNVLDTLFERIESISSICDSIHLTDSVLG
                     IPRISPLDIAKKIREYNQKIKLTCSLRVRDKELNTIEEIVKESIGIVDGILILMGDKS
                     NTNNDKTNLVSSKVVELLNDKGFNDKLELFLSIPANPNFAKIQKKINARPKGFFTQIV
                     NSQAMVQRITDYLKPKGFRIIPCLLLPSEKNLKSAEFLKIDWSNYKDNFIEFANEIHK
                     LSDDILISSPNDFQMAQTYLFKMKS"
     gene            2084..3034
                     /locus_tag="QGF01_03365"
     CDS             2084..3034
                     /locus_tag="QGF01_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HJN19966.1"
                     /translation="MLTESLLEALEKRILLFDGAMGTEIQKLDPNPEDFPDGKDGFND
                     GLSFSKPEWIKKIHRSYLEAGADCIETNTFGSNKIKLIEYGFGDKTVEFNQKNAEMAV
                     SICKEFSDKQRFVVGSMGPTGFLPSSNDPDLGQKSLEEIRNAFELQAEGLILGGVDAL
                     LIETSQDILEVKLAIEASHNAIKKTGKKVTIIANTTLDQYGKMLLGTNVQAAYTTVCD
                     MGIDLFGLNCSTGPIEMEPSIRWLNDQKHKKLLVVPNAGMPENEGGQAVYKMTPKNMA
                     DTLSSFLDEYPQIKIIGGCCGTGPAHITELRKVIDEKKKE"
     gene            3074..5572
                     /locus_tag="QGF01_03370"
     CDS             3074..5572
                     /locus_tag="QGF01_03370"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HJN19967.1"
                     /translation="MVKQRVSSALKSVDLKQEVGPLLIGERINTQGSKKAKQLVMNDD
                     FDGLIELARNQVDDGAHCLDVCVATTERSDEKEFMLKLAKHLSLEIDAPLVIDSTDPS
                     VIEKAIEQIPGKPIINSINLEGDGSRFKSLAPIMAKYGIPAIALCIGPNGMAKTPEQK
                     LETAELLYESGKKYGLKIEQFIFDVLTFTLATGEEEFQNAGKNTLEGIRLVKERFPNS
                     FTTLGLSNISFGLSPQSRRVVNSVFLYHAVKAGLDSAIVNVKEITPYTDIDENEKKLV
                     ENLIFNKSPDALSELITHFEKIISGSAKSERKTDIDPSWSAGKKANFRIVSRLKDGIE
                     NDVVSAIAEKISVNVIDSNGKLSLNAKKEVTHPGAIQTLNEDLLPAMKEVGDKFGSGE
                     LILPFVLKSAECMKAAVKELENYLVKEEGTSKGKLVIGTVYGDVHDIGKNLVKTIFVN
                     NGYEVYDLGKQVPLQKFVEKIDEVKPDAIGLSALLVSTSKQMQYFVEYSRKTNADIPI
                     LCGGAAINTNFINRIAKEGGIYNPGVFYCNTMFEGLKTMDKIVSNEREKFVEEWKNNI
                     EKWEERVIDNKKQIEKLPRSEIKPVKPPVPPTINQTIVINPEEIDLNEIWKNLHKKSL
                     FKLSWGIRGNSDVEAVIDQEKLLEEWKKRVVDDNLFEPKAVYGYFKCSCDDNNLVVKN
                     VDGGKLIFDFPRSSKSKHLCLSDYFGDDDVVAFQVVTVGNKVAETIEKWNNEDKYTDA
                     YYLHGLAVETAEAMAEWLNLKIKNELNLKNNAGLRYSWGYPSCPDISQHHLVWKLLHP
                     ENLGLKLTEVGQIIPEQSTAAIIIHHPDAEYFVL"
     gene            complement(5614..6096)
                     /gene="leuD"
                     /locus_tag="QGF01_03375"
     CDS             complement(5614..6096)
                     /gene="leuD"
                     /locus_tag="QGF01_03375"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214804.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HJN19968.1"
                     /translation="MKGKVIKYEQDNIDTDVILPGPYLKLHEHSELAEHAMEGMDPDF
                     HAKVKNANIIVTGNNFGCGSSREHAPIALSASGIEAVLALSFARIFYRNSVDGAYLLP
                     IEIDEKTYSSISENDELEIDTDNNQIKNITKNQTYSMKPFPDLIGKIISAGGLFKYKS
                     "
     gene            complement(6096..7364)
                     /locus_tag="QGF01_03380"
     CDS             complement(6096..7364)
                     /locus_tag="QGF01_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HJN19969.1"
                     /translation="MNITEKILARASSKDNVEPGDVQFANIDKIMLHDVSGPGVIKTF
                     EKLEKEGIIKVDKLFDPSMIWVTEDHFVPSVDKISAENIAKLSKFTNKFGITKHFKYG
                     MGQYGICHTMSHEEGLVSPGELYVGGDSHTNTTGALGAFACGLGHTDIAYAILHGELW
                     FKVPETLYFKLNGKLPENVMAKDFILRIIGDITTEGAAGKAMQFGGSGISDMSVESRL
                     TLTNMTTEAGAKNGIIEPDQKVFDYISERGITKCDPVYDDDDAQYEKTFEYEASELEP
                     LVAKPFSPQNVSPVRETNIEIDKSYIGSCTGAKYEDLVAAAKILKGRTVKVRTEVLPA
                     TISIYKKATENGLIQIFIDAGAVVGPPTCGACCGAQMGVLAKDEVCVSTTNRNFPGRM
                     GDLESKAYLASPLVTAASAVTGKLTDPRDL"
     gene            7437..8210
                     /locus_tag="QGF01_03385"
     CDS             7437..8210
                     /locus_tag="QGF01_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HJN19970.1"
                     /translation="MALEVLSIKATRMQDKFDLYNEIRKLVKKNSVSLQNGDILVISS
                     KYVSNSQGRVLDQNSIKSSEKAKEISKKFSINDKLSEAVIRESDVVFGGVSGFVITSS
                     DNIMAPNAGIDKSNSQNKFILYPTDPYLLAEQIRRKFFLDDCIHVGIIIVDSRLMPAR
                     IGTVGVAIACSGIEPVLDRRATKDLDGNPLKVTFQAIADNLASIANHKMGEGDETLPL
                     AIIRNSGAKITDRVINPKEMAISYDECVYVRGLKNWDST"
     gene            8265..8834
                     /locus_tag="QGF01_03390"
     CDS             8265..8834
                     /locus_tag="QGF01_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19971.1"
                     /translation="MVEYLTKYPKTVSFVDVVCGSLKGIVSVKKDSSVEQLHIVVKKN
                     ANELFKMFSAEGFTKIKFEHKQPSQIGHGLSLKLKKPWEMHVRLVKLKKELVAIHAEV
                     EISRDYIQHLFGQRTPIVYEIENMLKKYQIEYKVWDNKIKKYVQTVFDNYKIKLATPN
                     IPVFAWKPMLFVISTVGLMYLWKYLDTVF"
     gene            8888..9424
                     /locus_tag="QGF01_03395"
     CDS             8888..9424
                     /locus_tag="QGF01_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19972.1"
                     /translation="MPDNPATANFKHYGISPWEIEVLYELFTKKFEITQKVIEQTDEN
                     FVSWLEIILPVQFSEEFFQWFEFKSWEKVKSIIKEMKRRRGSGNAIRVEIYFIGKPSV
                     RFVVDLKENHDFNGAMEKIDYVVELLQYHLSEKDVPKNLTEIIYKFNIDIGRWMLNTV
                     FAEDKKFVYVENSWKGIT"
     gene            complement(9417..9851)
                     /locus_tag="QGF01_03400"
     CDS             complement(9417..9851)
                     /locus_tag="QGF01_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19973.1"
                     /translation="MDIIEANKIFRKSIIKGFFEETLLTLDFKKSNIKHPNISGDGLM
                     QSLLLHIFFDVETGLDYPDGDEWFIVDFLFPYEIKIPDSIIGPDYFTTISTGTSKNFW
                     HHREMVRYKYGKTKKLSESLKFIDSKYKELHSLVEPLEKDLK"
     gene            complement(9898..10863)
                     /locus_tag="QGF01_03405"
     CDS             complement(9898..10863)
                     /locus_tag="QGF01_03405"
                     /EC_number="1.2.7.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018491 - 2-oxobutyrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0047553 - 2-oxoglutarate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HJN19974.1"
                     /translation="MAIKLGDYKTGVHNDWCPGCGDFGIVNAIQMSLAEMQIPKHKTA
                     IFSGIGCSGKTSHFINTYGVHTLHGRVLTFAQGAKIANPEMTIVAAGGDGDGLGIGAG
                     HFVAAGRRNVDMTYIIFDNAVYGLTKGQASPTLKLGEQTKSLSNPNPNYNVNPIALAI
                     SSGFTFVARSYSYDVRQLKDLIISAINHKGLAFIDVLQPCPTYNDLYTRDWFGGLDQV
                     NEETKKPTPRIYNLADTDYDPVVHYSTIDELNQKLGQALAKSLEWGNKIPTGVFYKNE
                     LITPYTKRITDKIPNYLENPAAQQKISKNGKPITDISKLLDSLIV"
     gene            complement(10853..12760)
                     /locus_tag="QGF01_03410"
     CDS             complement(10853..12760)
                     /locus_tag="QGF01_03410"
                     /EC_number="1.2.7.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018491 - 2-oxobutyrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0047553 - 2-oxoglutarate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HJN19975.1"
                     /translation="MVFDYSWLIGGPQGSGVDTAANIFSRVGAKLGYHVFGKREYHSN
                     IKGLHSYFVVRLSDNKVRSNVNRANMMIAFDAETMIRHGLSISKNGVIIYDSSIVNTS
                     VDQIPTIEADHRVRLDEFFKSKNMEPKVSSIIELAKESGVKVYPISFWDTLSNIADEL
                     KRPELSRMTRMFNVLGVSFSLGVLKAPIAPLLESIEEIFSAKPAIVDLNKKAANFAYN
                     YASAKFSDFNISLDETDKKPNTMLVQGHQGSALGKIVCGCRFQSYYPITPASDESEFL
                     ESNEILQVNEDRPGSTTIVQTEDEISAIGMAVGASLTGTRSATSTSGPGFSLMAETLG
                     WAGINEVPVVISLFQRSGPSTGLPTRHGQDDLLFAINAGHGEFPRIVYASGDVEDSFY
                     DTTKCFNFADVFQLPVIHILDKFIASSVVTCNRFEPDLVSIDRGKLLEKIDGDYKRFA
                     HSPDGISPRSKLGLENGVFWNTGDESDEHGHISEDPVNRIQMMDKRQKKVEQILTLVP
                     KNDQAVVYGKSDICIVSWGSPKGPILDAIEMHRKEGHEISFIDIKLLEPFPTEHIKSL
                     LKDSSVIIDIEANMTAQLGSLIRKNLLKDPDYYVLKYTGRPMTCIEIFDSLKKILEKK
                     AEKRQVLLYGD"
     gene            complement(12774..12932)
                     /locus_tag="QGF01_03415"
     CDS             complement(12774..12932)
                     /locus_tag="QGF01_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19976.1"
                     /translation="MSADNTNEEFTTCDDCKNPIEKCTCVCPFCGERDKCECGLKLSN
                     NDGAATGG"
     gene            complement(12980..13240)
                     /locus_tag="QGF01_03420"
     CDS             complement(12980..13240)
                     /locus_tag="QGF01_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19977.1"
                     /translation="MLPDKCSIREGSRDCVNPPEFLITVVTGNDEFMLGITCQKHKTV
                     VLSKIENLQTQGNIPNGSIKFENLKSVQTDCVRGDPDDFIQL"
     gene            complement(13264..13881)
                     /gene="pdxT"
                     /locus_tag="QGF01_03425"
     CDS             complement(13264..13881)
                     /gene="pdxT"
                     /locus_tag="QGF01_03425"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HJN19978.1"
                     /translation="MTESNVGILALQGDVTENFMATMAALHDAGMDATVSQVKTPDQI
                     SKLDGLIIPGGESTMMGQLSMVNGAMNALKEIIDGGTPVFGICAGMILLSKNSTDRVM
                     GSTEQPLLDMLDVEIERNSFGRQKDSFQAEISLDPIGISSFQGVFIRAPAILTSGSNV
                     EVLSKFNEKIIAVKQGNILATSFHPELTQDISLHKHFVEMIKESN"
     gene            complement(13878..14846)
                     /gene="pdxS"
                     /locus_tag="QGF01_03430"
     CDS             complement(13878..14846)
                     /gene="pdxS"
                     /locus_tag="QGF01_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HJN19979.1"
                     /translation="MIPLSGDIPNAKGIKSDSIDSIDVTKGTSTLKRGFAHMLKNGVV
                     MDVTTVEQAQIAEEAGAVSVMVLDKLPSDVRKAGGVARTASIRIIQDIMDNVTIPVMA
                     KCRIGHVYEAKVLAEANVDMIDESEVLTPADENHHIWKWDYTTPFVNGARSLAEALRR
                     IEEGAAMIRTKGEPGTGNVAEAILHIKKVNDELRTIKSIFDSGDKQDLVRMAREFKVS
                     FDLVEETARIGRLPVVNFAAGGIATPADAAYLMSLGCDGIFVGSGIFKADDARERARA
                     VVLATTFWEDPKAVKEAQKMINEKQSMLGLDVDNLELRMQDRGGSA"
     gene            complement(14905..15189)
                     /locus_tag="QGF01_03435"
     CDS             complement(14905..15189)
                     /locus_tag="QGF01_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HJN19980.1"
                     /translation="MIIAIVDITPKEERMVEFKSWISDSNKVLSKFDGFVSRRLLEGE
                     DGSKRIVVEFENMEQFRKMHQSPEHTKFHSELGSFMQKPPQRQFFHIIAE"
     gene            complement(15255..15530)
                     /locus_tag="QGF01_03440"
     CDS             complement(15255..15530)
                     /locus_tag="QGF01_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19981.1"
                     /translation="MSTPYDDSVQKEWKERKGKIEDYYRDMGKVGTVDSKKISSISVV
                     KSGDKLVVQFVDGDGILYEITEDLVNPLKMIKTLSHAEIEKFKSLTK"
     gene            complement(15570..16001)
                     /locus_tag="QGF01_03445"
     CDS             complement(15570..16001)
                     /locus_tag="QGF01_03445"
                     /inference="COORDINATES: protein motif:HMM:NF022072.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HJN19982.1"
                     /translation="MPKFKFVKTVDANRDKIFKIITDFENLAKIFPKYFKSLKIISKS
                     GNITKLQEDLEFLGKKLSVTVDHVVEEPDKHIVIMLDGPGKDTRFEETFEKVPEGTKI
                     TVEVDLVLNGKFKVMGFFAKKKIKSKMSEALDEFAEVAKND"
     gene            complement(16007..16738)
                     /locus_tag="QGF01_03450"
     CDS             complement(16007..16738)
                     /locus_tag="QGF01_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19983.1"
                     /translation="MKKTFLIFLGIIFLFFNIDTVSAHQLIFSDGMNTNIDNSLYIPD
                     PQISWAMYGEVENNVLFYKFVAEQKEPFYASVVIPALDGLEEFHPSLAIISERQNIGL
                     ISLQSVENNTDLPFELAEKFNAIVFDYDSEFPGRVSYEPFTQVNYWERQEIRIDSINP
                     GTYYLAVFDTSENGKFTLAVGEIEDFRNANFFDSFIRAWFETKIFFEDYLTAFVSILI
                     TIAIFSGIGYGIFRAGRRIIRKSQR"
     gene            complement(16735..17661)
                     /locus_tag="QGF01_03455"
     CDS             complement(16735..17661)
                     /locus_tag="QGF01_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HJN19984.1"
                     /translation="MEPQRIVTFLPSVTELIYSLGADNKLFGVTHECNYPSDAKTKPR
                     VINSVFDPASMSSKQIDDKICQLMTDGKEIYNLNKENLLNAKPDLIISQNICEVCSAH
                     TEHVKLAVEMLEKKPEVYTMDPHDVSEILVSIKEISAMIGKEKEGNEIADSLTKRLEF
                     VKSQMFEKRPKVVAIEWVDPFFTSGHWIPEMIQIAGGENLISSEKMPSRKMKLEEIKE
                     ANPDIIVMMPCGFNVKRTVSEYNNVLAKNPDWNELKAIKENNVYAVDANSYFSKPSLR
                     TITGIEILAKITHPDIFNDLQLPKDSFMKIEI"
     gene            17754..17921
                     /locus_tag="QGF01_03460"
     CDS             17754..17921
                     /locus_tag="QGF01_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN19985.1"
                     /translation="MDAIPLSEQYRINIKDAIELIDNTLESVEKSNVILSGKHLREIR
                     KAISKKPYQKL"
     gene            complement(18011..>18243)
                     /locus_tag="QGF01_03465"
     CDS             complement(18011..>18243)
                     /locus_tag="QGF01_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HJN19986.1"
                     /translation="IPNVTAMMFKRSFKQTIILSMSFSVFSTVAGIMISYPLDIAPSG
                     MVVLLAITLFIGSLIMKSAGMIKTTGIQPKIH"
BASE COUNT         5868 a         3254 c         3081 g         6040 t
ORIGIN      
        1 actactccct tttttctctt ccgctactcc ctttgtttct agaattactt ttttgcttta
       61 gcagctggct ttgctttagc agctggcttt gctttagcag ctggctttgc tttagcagct
      121 ggctttgctt tagcagctgg ctttgcttta gcagctggtt ctactggttc tgactcaact
      181 acttctgatt caataactgg ctctggtaca ggttttattt tctcatcttt tttcttctct
      241 tggtgttctt ctccttcata ttttgaaatc agaatatatc cttcatcagt ttttgtcatt
      301 ctgactcgta cttttcttgg agggtgttta attcctctag accaaagtag atggctaaga
      361 tcttcttcaa ttttgatttg ttccattttc atatgacggc gtgcaaattc ctttaccata
      421 ttgatggcac gcttagcacg ttgattatta ggtgtgagta ataccttacc caaatttact
      481 gtataaacac gctcagattc ttgagacatt atcctacctc aacatcagtt tgtctccaag
      541 cccgtctttt tggatttgtt cttactttct tgttagttct aagcataatc catgctggta
      601 ctgaagatgt ctgttttatc tttttcatga ggcgtatctt tcttcctgaa tgtttgcgtg
      661 ctgccatatt gcacaaacca aattaagctc tttttaaatt aatctaaaac gatataatct
      721 gactttacaa caattttttc ctattcaata ggatattaag ttgccttaac attcttgctg
      781 atgcacccca aacaatatgt ttttcgaaag taagtgtata catttctttt atagaattat
      841 gttcgtgatt tgtatcattt gatatcgtct ctaaaaaaga gaaaagaggt atgtgcaaaa
      901 ccgattcaac ttctgaatta ggtttaagat taggaatttt atctataata gaaatgaatg
      961 gtgctattgt atattttgaa ttaagagtag ttacattatc cagttgccct aatatctgat
     1021 tcctatcaat tgataatgat aattcctcat ttgtttcacg aattgcagtt tctaataaat
     1081 catgatcact gctttgccat ttgccacctg gaaatgaaat ctcaccgcca tgttgtttca
     1141 aagtttttgc cttttttgtc atgagaatta ttggatcatg accgtagatg ataatcaaaa
     1201 ctgcagcaag tatgtttcca tcagcaggaa attgtacggg agaaattttg ctggtcaatg
     1261 cggtgcggat tccatcaata tccataatat cgaaacaggt ttgttcgtta tgggtttatc
     1321 gctcaaagtt ttaatcatgc tgtagcaaaa actattctat gacaataatg tatgagatca
     1381 atcctcccag aatttcagcg gaacagaatg ttctagatac gctttttgag agaattgaat
     1441 cgatcagttc tatatgtgat agcatccatc ttactgattc agtgttagga attccgagaa
     1501 tttctccgtt ggatattgca aaaaaaatta gagagtataa tcaaaagatc aaactgactt
     1561 gtagtttaag agttagagat aaagaattaa acactattga agaaattgta aaagaatcaa
     1621 ttggtatagt tgatggtata ctaatcttga tgggagataa atcaaataca aataatgata
     1681 aaacaaattt agtttcaagt aaggttgtag aacttttaaa tgataaaggg tttaatgata
     1741 aacttgaatt gtttctttca atcccagcaa atcctaattt tgcaaaaatt caaaaaaaaa
     1801 ttaatgcaag accaaaagga ttttttacac agattgttaa ttcacaagca atggtacaac
     1861 gaataactga ttacctcaaa ccaaagggct ttaggattat cccttgttta cttttacctt
     1921 cggaaaaaaa tttaaaatct gcagaattcc ttaagattga ttggtcaaat tataaagata
     1981 attttatcga gtttgccaat gagatacata aattgtctga tgatattcta ataagttcgc
     2041 caaatgattt ccaaatggct caaacatatt tattcaaaat gaaatcttga ctgaatcgct
     2101 gttagaagca ctcgaaaaaa gaattttgtt gtttgatggt gctatgggta ctgaaattca
     2161 gaaactcgac ccaaacccag aagattttcc tgatggaaaa gatggattca atgacggtct
     2221 ttcattttcc aaacctgagt ggataaaaaa aatacatcga agttatttgg aggctggtgc
     2281 tgactgtata gaaactaaca cctttggttc aaacaagatc aaattaatcg aatatggttt
     2341 tggtgataaa accgtggaat ttaaccaaaa aaatgctgag atggctgtaa gtatctgtaa
     2401 ggaattttct gataaacaaa gattcgttgt aggatcaatg ggtccgactg gatttttgcc
     2461 gagctcaaat gatccagatc ttggacagaa atcacttgaa gaaatacgta atgcatttga
     2521 acttcaagct gagggattaa ttcttggagg ggttgatgca ctattaattg aaactagtca
     2581 agatatactg gaagttaaat tagcgattga agcctcacat aatgcaataa aaaaaactgg
     2641 taagaaggtt acaataattg caaatacaac tcttgatcaa tatggtaaaa tgttacttgg
     2701 aacaaatgtt caggctgcat acactacagt atgtgacatg ggcattgatc tatttggctt
     2761 aaattgttcc acaggtccaa tcgagatgga accaagtatt cgatggttaa atgatcaaaa
     2821 acacaaaaaa cttttggtag tccctaatgc aggaatgcct gaaaatgagg gtgggcaagc
     2881 agtatacaaa atgactccaa aaaatatggc agatacgtta agctcatttt tggatgaata
     2941 tccacaaata aagataatcg gtggttgctg tggaactggt ccagcacata ttactgaatt
     3001 acgaaaagta attgacgaaa aaaagaaaga atgatgagtt aagtatttag aaaaaatcaa
     3061 ttaattgtaa tatatggtaa aacaacgagt tagttctgcg ttaaaatctg tagatctcaa
     3121 acaggaagtt ggtccgcttt tgattggaga gagaatcaat acacaaggtt caaagaaagc
     3181 aaagcagcta gtcatgaatg atgactttga tgggttgatt gaacttgcaa ggaatcaggt
     3241 tgatgatggt gcacattgtc ttgatgtatg tgttgcaaca actgaacgtt ctgatgaaaa
     3301 agagttcatg ttaaaacttg caaaacatct tagtttagaa attgatgctc cactagtaat
     3361 tgactcaact gaccccagtg ttattgaaaa ggcaattgaa caaattccag gtaaaccaat
     3421 aattaattca ataaatcttg aaggcgatgg tagtagattc aagtcacttg cacctataat
     3481 ggcaaaatat ggaattccag caattgctct atgcatagga cctaacggaa tggcaaaaac
     3541 tccagaacag aagttagaaa ctgcagaact tctttatgaa tcaggtaaaa aatatggatt
     3601 aaaaatagaa caattcattt ttgatgtttt aacatttaca cttgcaactg gagaagaaga
     3661 atttcaaaat gctggtaaaa atacattgga aggaataaga cttgtgaagg aacgttttcc
     3721 aaattcattt acaacgcttg ggctaagtaa cattagtttc ggtttatcac cacaatctag
     3781 aagagtggta aattcagttt tcctatatca tgctgtaaaa gctggattag acagtgcaat
     3841 tgttaatgta aaagaaataa caccatacac agacattgat gaaaatgaaa agaaacttgt
     3901 tgagaactta atcttcaaca aaagcccaga tgcattatca gaattaatta cacattttga
     3961 gaaaattatc tccggttccg caaaatcaga aaggaaaact gacattgatc cctcatggtc
     4021 ggctggaaaa aaagcaaatt tcagaatagt gagtagatta aaggatggaa ttgaaaatga
     4081 tgtagtatcc gcaatagcag aaaaaatttc agtgaatgta atagatagta atggaaaact
     4141 atctcttaac gcaaaaaaag aggttacaca tcctggtgca atacagacat taaacgagga
     4201 tctacttcct gccatgaaag aggtaggaga caagtttggt tctggtgaat tgattttacc
     4261 ttttgtactg aagtcagctg aatgtatgaa agctgcagta aaagaacttg aaaattatct
     4321 tgttaaagag gaaggcacta gtaagggtaa acttgtcatt ggtacagttt atggtgatgt
     4381 acacgatata ggaaaaaatc ttgtaaaaac aatcttcgtg aataatggct atgaagttta
     4441 tgatcttggt aagcaagttc ctttgcagaa attcgttgaa aaaattgatg aggttaagcc
     4501 agatgcaata ggactttcag cacttttagt ctcaacttca aaacagatgc agtattttgt
     4561 agagtattca agaaaaacta atgctgatat ccctatactt tgtggtggtg cagcaatcaa
     4621 tactaacttc ataaacagaa ttgcaaaaga aggcggtata tacaatcctg gtgtatttta
     4681 ctgtaacaca atgtttgaag gtctaaagac aatggataag attgtttcaa atgaacgtga
     4741 aaaatttgtt gaagaatgga aaaataatat tgagaaatgg gaagaaagag taattgacaa
     4801 caagaagcaa atcgaaaaac ttccacgcag tgaaattaag ccagtaaagc ctccagttcc
     4861 accaacaata aaccaaacaa ttgtgataaa tccagaagag attgatctta atgaaatatg
     4921 gaagaatttg cacaaaaaat cattattcaa actatcatgg ggcataagag gaaattcaga
     4981 tgtagaagca gtgattgatc aggaaaaatt actcgaggaa tggaagaaaa gagttgtgga
     5041 cgataatctg tttgaaccca aagctgttta tggttacttc aagtgtagtt gtgatgataa
     5101 caatttagtt gtgaaaaatg tggatggcgg aaaattaatt tttgatttcc cacgttcatc
     5161 caaaagtaaa catctctgtt tatccgatta ttttggagat gatgatgttg ttgcatttca
     5221 agtggtcact gtaggaaaca aagttgcaga aactattgaa aagtggaata atgaagacaa
     5281 gtacactgat gcttattatc ttcatggtct tgcagtagaa actgcggaag caatggctga
     5341 atggttaaat ttgaaaatta aaaatgaatt aaatctgaaa aataatgctg gtttaagata
     5401 tagttgggga tatccaagtt gtccagatat tagtcagcat catctagtct ggaaattatt
     5461 gcatcccgag aatttgggtt tgaaactcac agaggttgga cagattattc cagagcagtc
     5521 tactgctgca ataatcatac atcatccaga tgcagaatat ttcgttttat aattttattt
     5581 tagaattcta tgcgagaata aaaaatccaa tattcaagat ttgtatttga aaagaccccc
     5641 ggctgatatg attttaccaa tgaggtctgg aaatggtttc atggagtacg tttgattttt
     5701 tgtaatattt ttaatttggt tattatcagt gtcaatttct aattcgtcat tttctgaaat
     5761 actagaatat gttttttcat caatttctat gggtaaaaga tatgcaccat ccactgaatt
     5821 cctataaaaa attctagcaa atgacaatgc taatactgct tctattccac tcgcgcttag
     5881 agctattggt gcatgctccc tcgaagaacc gcaaccaaaa ttatttccag taacaataat
     5941 gttagcattt tttaccttgg catgaaaatc aggatccatt ccctccattg catgttctgc
     6001 taattcagaa tgttcatgta gtttcagata tggtccaggt aaaattacat ctgtatcaat
     6061 attatcctgc tcatatttga ttactttgcc tttcatcata aatccctcgg atcagttaat
     6121 tttccagtaa ctgcagaagc tgctgtaacc aatggtgaag caagataagc ctttgattcc
     6181 aagtctccca tacgaccagg aaaattacga ttggttgtac ttacacaaac ttcatctttt
     6241 gctaatacac ccatctgtgc gccacaacaa gcaccacatg taggaggacc tacaactgca
     6301 cctgcatcaa taaagatctg tattaaacca ttttcagttg cttttttgta aattgaaatt
     6361 gtggctggaa gaacttctgt tcttacctta actgttctac cctttagaat ttttgctgca
     6421 gcaactagat cctcatattt tgctccagtg caagaaccaa tgtatgattt atcaatctct
     6481 atattagttt ctctaacggg tgaaacattt tgtggtgaaa atggtttggc tacaagtggt
     6541 tcaagttctg atgcttcata ttcgaatgtc ttttcatatt gtgcatcatc atcgtcatat
     6601 actggatcac attttgtaat accacgttca gaaatataat caaatacttt ttgatcaggt
     6661 tcaatgattc cattttttgc acctgcctct gtagtcatat tagttaatgt caaacgtgat
     6721 tccactgaca tatcagagat tccacttcct ccaaactgca ttgccttacc ggcagcacct
     6781 tcagttgtaa tatcaccaat gattcttaaa atgaaatcct ttgccataac attttcaggt
     6841 aattttccat ttagtttaaa atacaaagtt tcaggaactt taaaccataa ttctccatgt
     6901 aaaattgcgt atgcaatatc tgtatgaccc aatccacaag caaatgcacc taatgctcca
     6961 gttgtattag tatgagaatc tccacccacg tacaattctc ctggtgatac aagaccttcc
     7021 tcatgagaca tggtatggca tattccgtat tggcccattc cgtatttgaa atgcttagta
     7081 attccaaatt tatttgtgaa tttggataat ttagcaatgt tttcagcaga gattttgtct
     7141 acagatggaa caaaatgatc ctcagtaacc caaatcattg aaggatcaaa aagtttgtct
     7201 acttttatga taccttcctt ttctaatttt tcgaatgttt tgataactcc tggtcctgaa
     7261 acatcatgta acatgatttt atcaatattc gcaaattgta catcacctgg ttcaacattg
     7321 tccttacttg acgctctggc tagaattttt tcagtgatat tcatacccaa aaatcgtttt
     7381 tcttcagatt aaaacctttt cagaataata aaaagtgagt gtgttataaa accagcatgg
     7441 ctctagaagt gctttctata aaggcaacaa gaatgcagga taaatttgat ctttataatg
     7501 aaattagaaa attagtaaaa aagaatagtg tttcacttca aaatggtgat attttagtta
     7561 tttcaagtaa atatgtctct aattcacaag gcagagtact agatcaaaat tctatcaaat
     7621 catcagaaaa agcaaaagag atatctaaaa aattttctat caatgataaa ttaagtgaag
     7681 cggtaatcag agaatctgat gttgtgtttg gcggagtttc tggatttgta attacatcat
     7741 ctgataatat tatggcacca aatgccggta tagataaatc aaattcacaa aataaattta
     7801 ttttgtatcc aacggatcca tatttacttg cagaacaaat cagaagaaaa ttctttttag
     7861 atgactgtat acacgttggt atcataattg ttgatagtag attaatgccg gcaaggattg
     7921 gaacggttgg tgtagcaatt gcatgttcag gaattgaacc tgttttggat agacgtgcaa
     7981 ctaaggatct tgatggtaat ccattaaaag ttacattcca agctattgcc gataatttag
     8041 catcgattgc aaatcataaa atgggagagg gtgatgaaac actaccattg gcaataatta
     8101 gaaattcagg tgcaaagatc actgacagag taattaaccc aaaagagatg gcaatttcat
     8161 atgatgaatg tgtctatgtc agagggttaa aaaactggga tagtacataa agtaacaaca
     8221 tattgagata ttagttttaa atatatgatg tcttcagaaa aattatggta gagtatctta
     8281 caaaatatcc aaagactgtt tcttttgttg atgtagtttg tggtagtcta aaaggaattg
     8341 taagtgtaaa aaaggattct agtgttgaac aactacatat tgtagtaaaa aaaaatgcaa
     8401 atgaattatt taaaatgttt tcagcagaag gtttcacaaa gatcaaattt gaacacaaac
     8461 aaccttctca aattggtcac ggtctttcgt taaaactaaa aaaaccatgg gaaatgcatg
     8521 ttagacttgt aaaactaaaa aaagagcttg tagccataca tgcagaagta gaaatttcta
     8581 gagattatat acagcatcta tttggacaac gaacaccaat agtatatgaa atagaaaata
     8641 tgctaaaaaa ataccagatt gagtataaag tatgggataa taagataaaa aaatatgttc
     8701 aaactgtatt tgataattat aagatcaaat tagcaacccc aaacatacca gtatttgcat
     8761 ggaaaccaat gctatttgtt atctctactg tgggattgat gtatctttgg aaatatctgg
     8821 atactgtttt ttaaactaga atttttttct gtttaaactt taattacaaa tttctttttt
     8881 ttatattatg ccagataatc cagccacagc caatttcaaa cattatggaa tttctccatg
     8941 ggaaatagaa gtgttatatg aattatttac caaaaaattt gaaattacac aaaaagtgat
     9001 tgagcaaact gatgaaaatt ttgtaagctg gttagaaata atattacctg tacaatttag
     9061 tgaagaattt ttccaatggt ttgaatttaa aagttgggaa aaagtcaaat caatcatcaa
     9121 ggaaatgaaa agacgtcgtg gtagtggaaa tgctatcaga gttgaaattt attttattgg
     9181 taagccatca gttaggtttg tagtggatct caaggagaat catgatttta atggtgcaat
     9241 ggaaaaaatt gattatgttg tagagttact acaatatcat ctaagcgaga aggacgttcc
     9301 aaaaaatcta actgaaatta tttataaatt caatatagat ataggaagat ggatgttgaa
     9361 tacagttttt gcagaagata aaaaatttgt ttatgttgaa aattcttgga agggtatcac
     9421 ttaagatcct tttctaatgg ttctactagt gagtgaagtt ctttatactt actatcaatg
     9481 aattttaatg attcagagag ttttttcgtt ttgccatact tgtatctaac catttcacga
     9541 tgatgccaaa aatttttact agtgccagta ctaattgtag taaaataatc tggaccaata
     9601 atactatcag gaatttttat ctcataagga aataaaaaat caacaataaa ccattcatca
     9661 ccatcaggat aatctaaacc tgtttcaacg tcaaaaaaaa tgtgaagtag aagagattgc
     9721 attagtccat cacctgaaat attaggatgt ttgatgttag attttttgaa atctagagtt
     9781 aggagtgttt cttcaaaaaa acctttgatg atggattttc taaaaatttt gttagcttca
     9841 atgatatcca tgtgtaatta gatgggaata ctatctaata ttcattatat cagttaatta
     9901 aacaattaag gaatcaagaa gttttgatat gtcagtaatt ggttttccat tttttgaaat
     9961 cttttgttgt gctgcaggat tttcaagata gttaggaatc ttatctgtga ttctttttgt
    10021 gtatggtgtg atcaattcat ttttgtaaaa tacaccagta ggtatcttat ttccccattc
    10081 aagtgatttt gccaatgcct gacctaattt ttgatttagt tcgtcaatag tgctgtaatg
    10141 tactactgga tcataatcag tatctgctag attgtaaatt ctaggtgttg gcttttttgt
    10201 ttcttcgtta acttgatcaa gacctccaaa ccagtctcta gtgtaaagat cattataggt
    10261 aggacatggt tgtaaaacgt caatgaatgc tagaccctta tggtttattg cagatatgat
    10321 gagatctttt agctgtctaa catcatatga ataacttcta gcaacaaatg taaaaccact
    10381 agaaattgct aaagctatag gattcacatt ataatttggg ttgggatttg ataatgattt
    10441 agtttgttct cccaacttca aggttggtga tgcttggcct tttgttagtc catatacagc
    10501 attatcaaat atgatatagg tcatgtcaac atttcgtctt ccagctgcaa caaaatgtcc
    10561 agctccaatt ccaagaccat caccatctcc accagcagca actatcgtca tttcaggatt
    10621 tgcgattttt gcaccttgag caaatgtcaa aaccctacca tgtagagtat gaacgccata
    10681 tgtgttgatg aaatgtgatg tctttccaga acaaccaatt ccagagaaaa tagctgtttt
    10741 atgtttaggg atttgcattt ccgccaatga catttgtatt gcattaacta ttccaaaatc
    10801 tccacatcct ggacaccaat cattatgtac tccagtctta taatctccta acttaatcgc
    10861 catacaacaa cacctgtctt ttttcagctt tcttttctaa tatttttttc aacgagtcaa
    10921 aaatttctat acaggtcatg ggtcttcctg tatattttaa aacataatag tctggatcct
    10981 ttaacaaatt ttttctgatc aatgagccaa gttgagcagt catatttgct tcaatgtcaa
    11041 ttataactga agaatctttt agaagtgatt tgatatgctc tgtagggaag ggttctaata
    11101 gtttgatatc tatgaaacta atttcatgtc cttcctttct atgcatttca attgcgtcta
    11161 aaattggtcc ctttggagag ccccaactta caatacaaat atcagattta ccatatacaa
    11221 cagcctgatc atttttagga actaaagtaa gaatttgctc aacttttttc tgtcttttat
    11281 ccatcatctg aattctgttt actggatcct ctgagatatg accgtgctca tcactttcat
    11341 caccagtatt ccaaaaaaca ccattttcaa gaccaagttt tgatcttgga gaaataccat
    11401 cgggtgaatg agcaaaacgt ttgtagtcac catctatttt ttcaagtaat tttccacgat
    11461 caatcgagac caaatctggt tcaaatctat tacatgttac aactgaacta gcgataaatt
    11521 tgtcaagtat gtgaatcaca ggaagttgaa aaacatcagc aaaattgaaa cattttgtag
    11581 tatcatagaa actatcttca acatctcccg atgcataaac tattcttgga aattctccat
    11641 gacctgcgtt tattgcaaaa agtaaatcgt cttgaccatg tcttgttggt aatcctgtag
    11701 atggtccact tctttgaaat aatgaaatga ctactggcac ctcatttatt ccagcccatc
    11761 caagtgtctc tgccatcaat gaaaatcctg gtcctgaagt tgatgttgca gaacgggtac
    11821 ctgttagtga tgcacctact gccatgccaa tcgcagaaat ttcgtcctca gtttgtacta
    11881 tagtagtaga acccggtcta tcttcattta cttgtaaaat ttcatttgat tctagaaatt
    11941 cgctttcatc tgacgcaggt gtgataggat aatatgattg gaatctacac ccacaaacaa
    12001 tcttaccaag ggctgaaccc tgatgtccct gaactaacat tgtgtttggt ttcttgtctg
    12061 tttcatcaag tgatatatta aaatcagaaa attttgcaga tgcataatta taagcaaagt
    12121 ttgctgcctt tttgttcaaa tcaacaatgg caggttttgc tgagaagatt tcttctatgg
    12181 attccagtaa tggtgctata ggtgctttca aaactccaag tgaaaatgaa acacccaaca
    12241 cattaaacat acgtgtcata cgtgaaagtt caggtctttt caattcgtct gcaatgtttg
    12301 ataatgtgtc ccaaaatgaa attggataaa ccttaacacc actttcctta gccaactcaa
    12361 ttatgcttga aacttttggt tccatgtttt ttgatttaaa aaattcatct aatctgactc
    12421 tgtgatctgc ttcaatggtt ggaatttgat caacgcttgt attaactata gaagaatcat
    12481 aaattattac tccatttttt gaaatgctca aaccatgcct gatcatggtt tcagcgtcaa
    12541 aagcgatcat catgtttgca cggtttacat ttgaacgaac tttgttgtct gaaagtctca
    12601 ctacaaaata actgtgcaaa cctttgatgt tagagtgata ttctcttttt ccaaaaacat
    12661 gatatccaag ttttgcacca acacgtgaaa atatgtttgc tgcagtgtca acaccacttc
    12721 cctgtggtcc tcctattaac caactataat caaaaaccat aacaaatcaa cccttaacca
    12781 ccagtagctg caccatcatt attagaaagt tttaatccac attcacactt gtcacgctct
    12841 ccgcaaaaag gacaaacaca cgtacatttt tcaattgggt ttttacaatc atcacaagta
    12901 gtaaattctt cgttggtatt atcagcactc atagtttttt attgttcaga tattataaag
    12961 ggtttaagtt ttttttcaat cataattgaa taaaatcatc aggatctcca cgaacacagt
    13021 cagtttgtac tgatttcaaa ttttcaaatt tgatggaacc gttaggtata tttccctggg
    13081 tctgaagatt ctctattttt gaaagaacca cagtcttatg tttttgacat gttattccta
    13141 gcataaattc atcattacca gttactactg taattaaaaa ttctggcgga tttacacagt
    13201 ctctacttcc ttccctgata gagcatttgt caggtaacat tgaaaaaatc tggtgaactg
    13261 gtattaattt gattctttta tcatttctac aaaatgcttg tgtaaagaaa tgtcctgcgt
    13321 caattcagga tggaatgatg tggcaagtat gttaccctgt ttaactgcaa ttatcttctc
    13381 gttaaatttt gacaaaactt caacattcga tccagaggtc aaaatggcag gagcgcgaat
    13441 aaacactcct tgaaatgatg aaattccaat tggatccaaa gaaatttccg cttgaaaaga
    13501 gtctttttgc ctaccgaaag aatttcgctc aatttcgaca tcaagcatat caagcaatgg
    13561 ttgttctgtt gaacccatca cacgatccgt tgaatttttt gaaagcaaaa tcattcctgc
    13621 acatattcca aaaactggcg tccctccatc aattatttct ttgagagcgt tcattgcacc
    13681 attgaccatg gaaagttgtc ccatcattgt actttctcca ccaggaatta tcaagccgtc
    13741 aagtttggat atttgatcag gggttttaac ctgacttaca gttgcatcca ttccggcgtc
    13801 atgcaatgca gccattgttg ccatgaagtt ttctgttaca tcaccttgta atgctaaaat
    13861 acctacatta gattcagtca agcagagcca ccacgatctt gcattcttaa ttcaagatta
    13921 tctacgtcca accctaacat agattgtttc tcattgatca tcttttgtgc ttcttttaca
    13981 gccttaggat cctcccaaaa tgttgttgcc aatacaacag cccttgcacg ttctcttgca
    14041 tcatcagctt tgaatattcc agagccaaca aaaattccat cacatcccaa tgacatcaag
    14101 tatgctgcgt ctgcaggtgt ggcaattcct cccgctgcaa agttaacaac aggcaatctt
    14161 ccaatccttg ctgtttcttc aactagatca aaggaaacct tgaactctct agccattcta
    14221 accaaatctt gcttatcacc tgaatcaaaa attgatttaa ttgttcttag ttcatcatta
    14281 acttttttga tatgtaaaat tgcttccgca acattccctg ttcccggttc gcctttagtc
    14341 ctaatcattg cagcaccttc ctcaattcgt cttaatgctt cagccaatga tcttgcaccg
    14401 tttacaaatg gtgtggtgta atcccatttc catatatgat gattttcatc agcaggtgtt
    14461 aaaacctcag attcgtctat catgtccacg tttgcttcag ctaaaacttt tgcctcgtag
    14521 acatgaccta tcctacattt tgccatgaca ggaattgtaa cgttatccat gatgtcttga
    14581 attatcctaa tacttgcagt tcttgcaacg ccaccagctt ttcgaacgtc tgaaggaagc
    14641 ttgtccaaaa ccatgacaga aactgctcca gcctcttcgg caatttgtgc ttgttctacg
    14701 gtagtaacat ccatcactac tccatttttc agcatatgtg caaaaccacg tttcaaagtg
    14761 gaagttccct ttgtcacatc gatagaatct atactgtcag acttgattcc cttggcattt
    14821 ggaatgtcac ctgaaagtgg tatcatgttt tgaaatacac ttctctctat ttattctatt
    14881 tcggtcaagc ccaacatgga tatatcattc ggcaatgata tgaaaaaact gtcgttgtgg
    14941 tggtttttgc atgaaactac ccaattcaga atggaatttg gtgtgttctg gactttgatg
    15001 catttttcta aactgctcca tattttcgaa ttcaactact atgcgtttag agccgtcttc
    15061 accctcaagc agtcttcttg aaacaaatcc atcaaacttt gaaagaacct tattggaatc
    15121 ggaaatccat gacttgaact ctaccattcg ttcctctttg ggagtgatat ctacaattgc
    15181 aataatcata ctgaccagtt ttgctcgtca aataacttct tttctatgat tttcagtagc
    15241 ctaaatccat caaactactt cgtcaagctc ttaaatttct caatctcagc atgcgataag
    15301 gtcttaatca ttttcaaagg atttaccaga tcctcagtta tttcatagag tatcccgtct
    15361 ccatcaacaa attgaacaac taatttatct ccagatttta caacgctaat tgaactgatt
    15421 tttttactat caaccgtacc cactttaccc atgtcgcgat aataatcttc gatttttccc
    15481 tttctttctt tccattcttt ttgtaccgaa tcatcataag gagtactcat aatattctaa
    15541 gagttccgtt ttagataagt ttgttcgctc taatcgtttt ttgcaacttc tgcaaactcg
    15601 tcaagtgcct cactcatctt gcttttgatc tttttcttgg caaaaaatcc cattacctta
    15661 aacttgccat ttagcaccaa atccacctcc accgtaattt tggtcccttc cggtactttt
    15721 tcaaatgtct cctcaaacct tgtatcctta ccaggtccat caagcattat cacaatgtgc
    15781 ttgtcaggtt cctcaacaac atgatccacc gtcacagaaa gcttcttacc caaaaactcc
    15841 agatcctcct gcagttttgt aatatttcca gattttgata ttatttttag tgatttgaaa
    15901 tactttggga aaatttttgc cagattttca aaatctgtga ttatcttaaa gatcttatcc
    15961 ctgttagcat ctaccgtttt tacaaacttg aattttggca tgccaactat ctttgacttt
    16021 ttctaataat tcttcgccca gctctgaaaa ttccatatcc aattccagaa aatattgcaa
    16081 tggtaatcaa tatggataca aatgctgtga ggtaatcctc aaagaaaatt tttgtctcaa
    16141 accatgctcg tatgaaagag tcaaaaaagt tggcgtttct gaaatcttcg atctctccaa
    16201 cggctaatgt aaatttgcca ttttcagatg tgtcaaaaac cgccagatag tatgtacctg
    16261 ggtttatact atcgattcta atctcctggc gttcccaata atttacctga gtgaatggtt
    16321 cgtaagacac ccttcctgga aactcgctgt cataatcaaa aacaatcgca ttaaatttct
    16381 ccgccaattc aaatggcaaa tcagtattgt tttcaactga ctgcaaacta atcaatccaa
    16441 tattttgccg ctcagatatt attgctaaag atggatgaaa ttcttccaag ccatccagtg
    16501 ccggtataac aacactcgca tagaaaggct ccttttgctc agccacaaat ttgtaaaaca
    16561 gaacgttgtt ctcaacctcg ccatacattg cccacgaaat ctggggatct ggtatgtaaa
    16621 gagaattatc tatgtttgta ttcattccgt ctgaaaagat gagctggtgt gcggatactg
    16681 tatctatatt aaaaaacagg aatatgattc caagaaatat taaaaaagtt tttttcatat
    16741 ctcaattttc ataaacgaat cttttggtaa ttgcaagtca ttaaaaatat ctggatgagt
    16801 aatcttggct aggatctcaa ttccagttat ggttcgaagg ctcggcttgc tgaaatacga
    16861 atttgcgtca actgcataaa cattattttc cttaatggcc ttcagttcat tccaatctgg
    16921 attcttggca agtacattat tgtattcaga gactgtccgc ttgacattaa acccacatgg
    16981 catcattaca ataatgtcag gatttgcctc cttgatctct tccaacttca tttttcttga
    17041 tggcattttt tccgaactaa tcagattttc tccaccggct atctgaatca tctcaggtat
    17101 ccagtgacct gaagtaaaaa atggatccac ccattctatt gcaaccactt tgggtctttt
    17161 ttcaaacatt tgacttttga caaattcgag cctttttgtg agtgaatcag caatttcgtt
    17221 gccttccttt tccttaccaa tcattgctga gatctccttg atgcttacca agatttcact
    17281 aacatcgtgt gggtccatcg tgtaaacctc tggctttttt tccaacatct ccacagccag
    17341 tttcacatgt tctgtatgtg cagagcaaac ctcacaaatg ttctgggaaa taatcaggtc
    17401 gggtttagcg tttaggaggt tttccttatt gagattgtaa atctccttac cgtcagtcat
    17461 taattggcag attttatcat caatctgctt gctggacatt gaggctggat caaatacgga
    17521 attgataact cgtggtttag tttttgcgtc actcggataa ttgcattcat gtgttacccc
    17581 aaaaagtttg ttgtcggcgc ctaggctgta aatcagctct gtcacactcg gcaggaaagt
    17641 gacaatgcgt tgtggctcca taactcatca ttatcaggca tcaaataaat ctgatttgac
    17701 aatcgtagaa ttcaagaaag aatatgtaga agtattatga aatatgattt agaatggacg
    17761 caattcctct ttcagagcaa tatagaatta acatcaaaga tgctatagaa ctaattgata
    17821 atacgcttga atcagtggaa aaaagtaatg ttattttatc aggaaagcat ttgagagaaa
    17881 tcagaaaagc gatatcaaaa aaaccctacc aaaagttatg aaaaaagggt aaaattaatc
    17941 tcaattatta gctttttaac ctctattttc cactttttaa catcaatttt gaggttttag
    18001 tatctaattt ctaatgtatc tttggctgga ttccagtagt ctttatcatt ccagctgact
    18061 tcattatcaa agaaccaatg aagagcgtta ttgctaataa cacaaccatt cctgatggtg
    18121 caatatctag cggatatgaa atcattattc ctgccactgt ggaaaatact gaaaatgaca
    18181 ttgaaagaat aattgtctgt ttaaatgaac gtttaaacat cattgcggtg acatttggaa
    18241 tta
//
LOCUS       DASCPJ010000076         3894 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001204816, whole genome shotgun sequence.
ACCESSION   DASCPJ010000076 DASCPJ010000000
VERSION     DASCPJ010000076.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001204816"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            174..1529
                     /locus_tag="QGF01_03470"
     CDS             174..1529
                     /locus_tag="QGF01_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HJN19987.1"
                     /translation="MDIFGHTSTPRIISLDYSGTQFEIYNKISRNYPYSFLFESLTGP
                     EELAETSIMGFDPEFIVKGYYDKVTIQNRDGTIKTISTSDPLDTIKDLVQKTTDQSYR
                     YLGGAVGIINYDSIRLWEDIPKHHDISEPIMEFGIYNDGILFDNKKRKSLYFYYDKNR
                     VDEIKSSESKFEEFTISKITNNLNEEKFSEIVNKAKKYIYDGDIFQVVLSRKFSFEAD
                     GDYLKVYQKLRSLNPSPYLYHLKMNEKTVIGSSPEMLLRVTNDHVETFPIAGTRKITN
                     DEKKNEEMKQELISDEKELAEHTMLVDLGRNDIGKVCEYGSVHVKELMEVKKFSHVQH
                     MVTHVVGKLGKDYDMYDAFRAVFPAGTVSGAPKVRAMEIIDELEPESRGSYAGAIGYF
                     SFNGCCDFAIAIRSIFANGNLGFVQAGAGIVFDSISENELKETEHKANAMLTALKEAA
                     E"
     gene            1526..2116
                     /locus_tag="QGF01_03475"
     CDS             1526..2116
                     /locus_tag="QGF01_03475"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HJN19988.1"
                     /translation="MKFLIVDNYDSFVYNIAQRLGELGVNSDVIRNDRITIDEIKEKN
                     YDAIIISPGPGTPEEKRYFGICKDIIQKLGPKLPILGICLGHQGIIDCFGGKVVNAGN
                     VRHGKTSQIQHYDDSLFHGVKNPFRATRYHSLVGDKTIIPDSLKITAIAEDDGEIMGV
                     SHKEYFIDGVQFHPESIMTDEGKKILENFITRVKND"
     gene            2109..3155
                     /gene="trpD"
                     /locus_tag="QGF01_03480"
     CDS             2109..3155
                     /gene="trpD"
                     /locus_tag="QGF01_03480"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HJN19989.1"
                     /translation="MIKELTEQLSLGTNLTIDQMSNVMDEILTGTQNDDNVAEFLKNL
                     TQKGESDDELLAMLNKMEEFSLHISPNCKGKIIDVCGTGGDKLKTFNISTAASFVIAG
                     AGGNVAKHGNRSISGISGSADIFEYFGFDLNSEPNKVNETIEKFGIGFMFAQKFHPAM
                     KNVAKARKIVGGRTAFNLLGPLTNPAMVKNQLIGVFSEKYQEKIVKILQRKNAETVMA
                     VRSADGMDELSTTCKNQICMLKNDTITKMTLDPQKVGLQKGNITDIQIRSKEDAIKSF
                     ITVLNNTANKTKIEITALNAAGGLVISDIADNFTDAVELALDTIHSGKAFDKLKNFVK
                     NYGDIEKLDGAEKL"
     gene            3152..>3894
                     /locus_tag="QGF01_03485"
     CDS             3152..>3894
                     /locus_tag="QGF01_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HJN19990.1"
                     /translation="MKNVLEKLVVNSRKAIDNGIYDIIETLPKSEIDLEESIRNNVHA
                     SLITEIKFSSPAEGNIRQISDPLQIAESMISGGAKALSVLTQPYLFHGSPEYFIKIRK
                     NVKIPLLMKDVMIDKIQIDAAKKMGADYFLLIQALFDNDFVSEIDELIDYGHKNGLKI
                     LLESHTKAEFDNAVKTNADIIGINNRNLDTLEINLETTKQILENYQESKMILSESGIK
                     SAEDVKFLRDCGADAFLIGTSIMKSSDIQ"
BASE COUNT         1496 a          521 c          703 g         1174 t
ORIGIN      
        1 atttataagt ataccgtacc atacggtaat ttcactctga ttttatgtat caaaaacagt
       61 gattatgata gtaaaaatac tgatttagta gttaaaaata cattttttga tacaaaaatt
      121 tgtataatag aaatcatgat ttaaatacag ttgaattttg tttatagaaa tgggtggaca
      181 tatttggaca cacgagcact cctaggataa tttctctaga ctattctggc acccaatttg
      241 aaatttataa taaaatatca cgaaactatc catattcatt cctgtttgaa tcattaactg
      301 gcccagaaga attagctgaa acttcaatca tgggatttga tcctgaattt attgtaaaag
      361 ggtattatga taaagttaca atccagaatc gagatggaac tatcaaaaca atttctacat
      421 cagatccatt agatacaata aaagatctag tccaaaaaac caccgaccaa tcatatcgtt
      481 atctcggagg tgctgttggg attattaatt atgattcaat tagattatgg gaagatattc
      541 caaaacatca tgatatatca gaaccaataa tggaatttgg tatttataat gatggaatat
      601 tattcgacaa taaaaaaaga aaatcattat acttctatta tgataaaaat agagtagatg
      661 agataaagag ttctgaatca aaatttgaag aattcacaat atcaaaaata actaataatc
      721 tcaatgaaga aaaattttct gaaattgtaa ataaagcaaa aaaatacatc tatgatggag
      781 atattttcca agttgtttta tcgagaaaat tctcgtttga agctgatggt gattatctta
      841 aagtgtatca aaaacttcgt tcattaaatc catcacctta tttatatcac ttgaaaatga
      901 atgaaaaaac cgtgattggt tctagtcctg aaatgctttt acgtgttaca aatgatcacg
      961 ttgaaacatt tcctatcgct ggcacaagaa aaattacaaa tgatgagaaa aaaaatgagg
     1021 agatgaaaca agaattaatc agtgatgaaa aagagttagc agagcataca atgctggttg
     1081 atttaggaag aaatgatatt ggtaaggttt gtgaatatgg ttcagtccat gtaaaagagc
     1141 tcatggaagt aaaaaaattt agtcatgtcc aacatatggt tacacatgtt gttggtaagc
     1201 ttggaaaaga ttatgatatg tatgatgcat tcagagctgt cttcccagct ggaactgttt
     1261 caggtgcacc aaaagtaaga gcaatggaaa taattgatga attagaacct gagtcaagag
     1321 gatcatatgc tggtgcaatt ggatattttt catttaatgg atgctgtgat tttgcaattg
     1381 caatcagaag tatttttgct aatggaaact tgggctttgt gcaggcaggt gctggaattg
     1441 ttttcgattc aatttcggag aatgaattga aagaaactga gcataaagct aatgcaatgt
     1501 tgactgcatt aaaggaggca gcagaatgaa atttcttatt gttgataatt atgattcatt
     1561 tgtttacaat attgcccaaa gattaggtga attaggtgta aattctgatg ttataagaaa
     1621 tgatagaatt actattgatg aaataaaaga aaagaattat gatgcgataa ttatctcacc
     1681 gggtccagga acccctgaag aaaaaagata ttttggaatt tgcaaggata ttatacaaaa
     1741 acttgggcct aaactgccaa ttctaggtat ttgtttaggt catcaaggaa taatcgattg
     1801 ctttggtggt aaagttgtaa atgctggaaa tgtaagacat ggtaaaacaa gccagatcca
     1861 acattatgat gattcacttt ttcatggagt aaaaaatcca tttagggcta ctcgctatca
     1921 ttcacttgtt ggagataaaa cgattattcc agattcatta aaaattacag caatagctga
     1981 agatgatgga gaaattatgg gtgttagtca taaagaatat ttcatagatg gtgtacaatt
     2041 tcatcccgaa tccatcatga cagatgaggg caaaaaaatc ttggaaaatt tcataacgag
     2101 ggttaaaaat gattaaagag ttaacagagc aactttccct tggaactaat ctaacaattg
     2161 atcaaatgtc taacgtaatg gatgagatac ttactggaac acaaaatgat gacaatgtag
     2221 cagaattttt aaaaaattta acacagaaag gcgaatcaga tgacgaactt cttgcaatgc
     2281 taaacaaaat ggaagaattt tctctgcaca tatcaccaaa ttgtaaagga aaaattattg
     2341 atgtgtgtgg tactggtggt gataaactca aaacattcaa tatctcaaca gcagcatcat
     2401 ttgtcattgc tggtgctgga ggaaatgttg ctaagcatgg taaccgttca atatctggca
     2461 tatcaggcag tgcagatatt tttgaatact ttggatttga tttgaattct gaacctaaca
     2521 aagtaaatga aacaatagaa aaatttggta ttgggtttat gtttgcacaa aaatttcatc
     2581 cagcaatgaa aaacgttgca aaggctagaa aaattgttgg cggcagaacc gcattcaatc
     2641 tattaggacc attaactaac ccagcaatgg taaaaaacca acttattgga gttttctcgg
     2701 agaaatatca agaaaaaatt gtaaaaattc tgcaaaggaa aaatgcagaa acagttatgg
     2761 ctgttagatc agctgatggg atggatgaat tatctactac atgcaaaaac caaatatgta
     2821 tgttaaaaaa tgatacaatt acaaaaatga ctctagatcc tcagaaagtt ggattacaaa
     2881 aaggtaacat cactgacata caaatacgtt caaaagagga tgcaataaaa tcattcatta
     2941 ctgtactaaa caatactgca aacaaaacaa aaattgaaat cactgcacta aacgctgctg
     3001 gtgggctagt gatatcagat attgctgata atttcactga tgctgttgaa ttggcattag
     3061 atactattca tagtggcaaa gcgtttgata aattgaaaaa ctttgtaaaa aactatggcg
     3121 acatagaaaa attagacggt gctgaaaaat tatgaagaat gttctagaaa aactagttgt
     3181 aaattcacga aaggctattg ataatggtat ttatgatata atagaaacat taccaaaatc
     3241 agaaatcgat ttagaagaat ctataagaaa taatgttcat gcctcactca ttacggaaat
     3301 aaagttttca tcacctgcag aaggcaatat tagacagatt tctgatcctc tacaaatagc
     3361 tgaatcaatg atatctggtg gtgcaaaagc actttctgtc ttaacacaac catatctttt
     3421 ccatggctca cctgaatatt ttataaaaat tagaaaaaat gtaaaaattc cattattgat
     3481 gaaagatgtt atgattgata aaatacagat tgatgcggca aaaaaaatgg gtgcagatta
     3541 ttttttactt attcaggcat tatttgataa cgattttgtt agtgaaatag atgaattgat
     3601 tgattatggt cataaaaatg gattaaaaat tttactagaa tcacatacta aagcagaatt
     3661 tgataatgca gtaaaaacta atgcagatat tattgggatc aataacagaa acttggatac
     3721 acttgaaatc aatcttgaga caacaaaaca aattttagaa aattaccagg agtctaaaat
     3781 gatattgtct gaaagtggaa tcaagtcagc agaagatgta aaatttttac gtgattgtgg
     3841 tgcggatgca tttcttatag gtacaagtat aatgaaaagc tcagatatac aaaa
//
LOCUS       DASCPJ010000077         2398 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001218911, whole genome shotgun sequence.
ACCESSION   DASCPJ010000077 DASCPJ010000000
VERSION     DASCPJ010000077.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001218911"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="QGF01_03490"
     CDS             complement(<1..875)
                     /locus_tag="QGF01_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJN19991.1"
                     /translation="MKIAAGIPAFNEEKNVGSVIVELLKFVDIVIVCDDGSNDNTGMI
                     AKKMGAIVVNHERNLGYGAGIKSLFLKARELGVDVLVTLDADGQHRPKDVLTVLEPIK
                     KHETDIVIGSRFLDQNQQQIPSYRKAGIKIITKLANTTLDKTITDSQSGFRAYNSNVL
                     SEIIPLEHGMGVSNEILIKANKKGFTISEVPIVVSYQGDTSTHNPVSHGASVIFSTLK
                     IISIENPFKLYGIPGSIFLAIGLFFTVLTIQDFTETKQMLLSTAIIGAATIIFGTILL
                     MTAIILYSVVNVIRE"
     gene            complement(880..2322)
                     /locus_tag="QGF01_03495"
     CDS             complement(880..2322)
                     /locus_tag="QGF01_03495"
                     /inference="COORDINATES: protein motif:HMM:NF015983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN19992.1"
                     /translation="MKYKQILLTRPPTGSNLLQGGTDKDPHPQPPLGLASIAGVLERQ
                     NDVNVLDILDGNFSDNYVYDVTQAVKKHNPDLVGFTAFTPFANPALEAITAVKEINPN
                     ILTVLGGPHAVLADVTMRKCPALDITVYDEGEEQIEEIVTGKPIDEIAGLFIRKDGRI
                     VPTKPRAYIANMDSLPYPAYHKLPHFPDGYHPHPPKSTGKRWCSIMWSRGCPFFCNYC
                     NRENSFGLKFRNQSPEYVVDHIQYLHSEYGIEELTFYDDVMTLNRKATMDLMDALHPK
                     KLGFKMSWDAETRVDLVDKELLLAMKEAGCRMMSYGIEHGEFIQEIKGGNATLEQSEK
                     AVKWAHEAKIQTAGYFMIGLPNETPETIRKTIDFAKKLDCTYATFSITMPFPGNKLYD
                     EAVKSGLIQLDDTWEKFVYGGVGVGGIQAPVLTTESLSANDLEYWTKKAFREFYLRPS
                     YIMKKLLSVRSIGDLMTYYNGYKMMKKDTV"
BASE COUNT          729 a          452 c          355 g          862 t
ORIGIN      
        1 ctttctctta ttacgtttac aacggaatat aatatgatag cagtcattag taaaattgtg
       61 ccaaagatta tggtagcagc tcctataatt gcagtactaa gtaacatttg ttttgtttca
      121 gtaaaatcct gaattgtgag tacggtaaaa aataaaccaa ttgcaagaaa aatggatcca
      181 ggtattccat aaagcttgaa cggattttct attgagatta tttttagtgt gctaaagatt
      241 acggatgcgc catgagatac aggattatgt gtagaagtat ctccttggta tgaaacaact
      301 attggaacct cagaaattgt gaagcccttt ttgttagcct ttattagaat ttcgtttgat
      361 acacccatac cgtgttctaa tggaataatt tctgataaaa catttgagtt gtatgcacga
      421 aacccactct gtgaatcagt gattgtttta tctaatgttg tatttgctaa ctttgttatt
      481 attttaattc ctgcttttct gtaagaaggg atttgttgtt ggttttgatc taaaaatcta
      541 gaacctatca ctatgtcggt ttcatgtttt tttattggct ccaaaacagt taggacatct
      601 ttcggtctgt gttgtccatc tgcatctaat gtaactaaga catcaactcc aagctctcta
      661 gcttttaaaa aaagtgattt tattcctgca ccataaccta aatttctctc atggttaact
      721 acaatagcac ccattttctt tgcgatcata cctgtattat catttgaacc gtcatcacaa
      781 acaataacaa tatcaacaaa tttgagtaac tctacaatta cagaaccaac atttttttcc
      841 tcattaaaag cgggaattcc agcagctatt ttcaaataat cacactgtat cttttttcat
      901 catcttgtaa ccattataat acgtcataag atcacctata ctccttacac tgagtaattt
      961 tttcataata tatgatggtc gtaaataaaa ttctctaaat gctttctttg tccaatattc
     1021 aagatcatta gcagataatg attcagttgt caaaactggt gcttgtattc cacctacacc
     1081 tacaccacca taaacaaatt tttcccatgt atcatctagt tgtatcaatc ctgatttaac
     1141 agcctcatca tacaatttat tcccagggaa tggcatggtt atactaaatg ttgcatatgt
     1201 acaatcaagt tttttagcaa aatcaatggt ttttctgatt gtttctggcg tttcatttgg
     1261 tagcccaatc ataaaatatc cagctgtttg aatttttgct tcatgtgccc atttaactgc
     1321 cttctcagat tgttcaagcg tagcatttcc acctttgatt tcttgtataa attcaccatg
     1381 ttcaattcca tatgacatca ttctacagcc agcttctttc attgctaaaa gtaattcctt
     1441 atcaacaaga tcaactcttg tttccgcatc ccaagacatc ttaaaaccta gtttttttgg
     1501 atgtaaagca tccatgagat ccatagttgc ttttcgattt aatgtcataa catcatcata
     1561 aaacgttaat tcctcaatac catattctga atgaagatac tgaatatgat caacaacata
     1621 ttctggtgac tggtttctaa actttagacc aaaactattt tctctattac aataattaca
     1681 aaagaatgga catcctctgg accacattat actacaccac ctctttccag tactttttgg
     1741 cggatgtggg tgataaccat ctggaaaatg aggaagtttg tgataagccg gatatggtaa
     1801 tgaatccata tttgcaatgt acgcacgagg ttttgttggt actatacgtc catcttttct
     1861 aatgaataag cctgcaattt catctatagg tttacccgtt acaatttctt ctatttgttc
     1921 ttctccttca tcatatacag taatatcaag agctggacat tttctcattg taacatctgc
     1981 aagtacagca tgaggtcctc caagtacagt taaaatattt ggatttattt cttttacagc
     2041 tgtaatagct tctaatgctg gatttgcaaa aggtgtaaat gccgtaaacc caaccaaatc
     2101 aggattatgt ttttttaccg cttgggttac atcgtaaaca taattatctg aaaaatttcc
     2161 atcaagtata tctagtacat ttacatcatt ttgtcgttca agtactccag ctatggatgc
     2221 aagtcctaat ggaggctgtg gatgtggatc cttatctgta ccaccttgta ataaattaga
     2281 accagtaggc ggtctagtta gaagtatttg tttatatttc atatatactc acagccgatt
     2341 tgttcttcat aacgtctaaa agtattacca tctaaaaaat tcatcatttt tgatattt
//
LOCUS       DASCPJ010000078        26262 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001240346, whole genome shotgun sequence.
ACCESSION   DASCPJ010000078 DASCPJ010000000
VERSION     DASCPJ010000078.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 26262)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26262)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26262
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001240346"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="QGF01_03500"
     CDS             <1..847
                     /locus_tag="QGF01_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJN19993.1"
                     /translation="IHLKDKLRISREEALDRAVKTVEYAKSHGLKIRFTVEDGCRADP
                     QFLIKVCKAIEEAGVDRISLPDTVGVLRPIGMYNFVKRVRSEVNVPLDAHVHNDIGFA
                     LANAFAACDAGADQIHTTIDGIGERTGIPSLAETAVALAYLYKSPNDFRLDMLVDLSR
                     LIEQYTTIKPYDSKPIVGESAYKHKAGTHLAAILRNPAAYEPIPPRTVGQTRKAVFGE
                     LAGKTGAEYLMSILGLEKNVENAKNVANGLKNLRMGDLIEVPLENNVERKINEEKMSK
                     GRKND"
     gene            840..1007
                     /gene="lysW/argW"
                     /locus_tag="QGF01_03505"
     CDS             840..1007
                     /gene="lysW/argW"
                     /locus_tag="QGF01_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HJN19994.1"
                     /translation="MTNCSECDADLSVPSDAIEGEIVTCAECGASFELVKAPNGFELK
                     PAQTVGEDWGQ"
     gene            995..1846
                     /gene="lysX"
                     /locus_tag="QGF01_03510"
     CDS             995..1846
                     /gene="lysX"
                     /locus_tag="QGF01_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HJN19995.1"
                     /translation="MGTVTSITILYDTIRLEEKLLIESAKKNNITIEMVDCKKLFIDL
                     DKNSHDIQTVLQRCVSYYRNIHSTAVLEGQGVKVVNCLNTGIFAGNKLFTHMLLKKAG
                     IETPNATVAFSKDAAMKMFGECGFPKIIKPTVGSWGRMVSKINDMDSADGIIEGKEAM
                     HPIHHIHFFEEFVQRPPRDIRVIVIGDNAVAAIYRNSADGNWKTNTHLGGSAETCKIT
                     NELEDVCLKAKNTVFGDIVGVDLMESEEKGLVVHEINNTTEFRNVVRVTGIDVPKLML
                     EHVKGLQ"
     gene            1848..2969
                     /locus_tag="QGF01_03515"
     CDS             1848..2969
                     /locus_tag="QGF01_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HJN19996.1"
                     /translation="MDTHLVSSKYAVKTLEKALRIYTPSLREKALADFLADRCDDLGF
                     KDIHQDEVGNLIAIKGSGEPRIMLCGHMDVVPGRIKVRKEGDYLFGRGASDAKSPLIA
                     MLFAASSMQENEGTVIFVGAVDEEGNATGIKNLVKNKPEIDYAIFGEPSSIQKVTIAY
                     KGRLAINLKISVENSAHASAPWLAKNAIEETATVVKDLKNNLEGGQENKTKGMMLTAS
                     LTEIRGGTSHNITPKECIATIDIRIPVDMNCNSVEEKITTSINEISSKRGIEILYSVV
                     DETEPFEAAHNSALVRAITLAVLDVEQKRPTLIRKTGTGDMNVVGNQLKIPVITYGPG
                     DPHEAHTIDEKVSIEEFLKGIDVFKRTLFHLKRLHGRSK"
     gene            2969..4006
                     /gene="dph5"
                     /locus_tag="QGF01_03520"
     CDS             2969..4006
                     /gene="dph5"
                     /locus_tag="QGF01_03520"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HJN19997.1"
                     /translation="MLWFIGLGISGTESIPSESIKIIEKADLVYLESFTSPIYVKHEE
                     EIKNLVHGEFKIAKRWMVEDGKEILNASRSSIVVLLSYGDPYIATTHIELRTRARLED
                     IETKTIHSTSAITAMIGEIGLQFYKVGKIVTIMSDKKSVTTPYTTIFKNLIEGLHSII
                     LLEYNEDQNFFLDPKTAILSLLDIEKEQKRKVVSNDTFAIVTSRVGMKTQKITSGKFS
                     DLLKMDFGEPPHSVIITGKLHFTESDAISALTDCLSKPADNSNDIKNISMQMIEKYVP
                     MVREALNEIKPFYSDAKEFQDILQNAELYIDDAENFLKEGKDENAVLSIGYADGLVDA
                     LRIAKGIDPKM"
     gene            4096..4521
                     /locus_tag="QGF01_03525"
                     /pseudo
     CDS             4096..4521
                     /locus_tag="QGF01_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
     gene            complement(4523..5212)
                     /locus_tag="QGF01_03530"
     CDS             complement(4523..5212)
                     /locus_tag="QGF01_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HJN19998.1"
                     /translation="MSDLKIQHILTLSQLLSKGARYNFIQITTSSLGKNIKKSQQSAS
                     KHLLELETNGFIERVVHGRKVSVKITQNGYAELLKLHLLLRANIETSVSSLELKGTII
                     SGMGEGAYYMSLKGYTKQFKSKIGFIPFPGTLNVKLNQPEYIQAVEQFIVLDGIMIEP
                     FSDGKRTYGWVKCFHAKLNNSTKCELIRLERTHHDNSVIELISKNNLRKTAKLKTGAK
                     VTIKILIPNRS"
     gene            complement(5219..6361)
                     /gene="dnaG"
                     /locus_tag="QGF01_03535"
     CDS             complement(5219..6361)
                     /gene="dnaG"
                     /locus_tag="QGF01_03535"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HJN19999.1"
                     /translation="MPYTGIVKYHVKLSFDVDGLVERADIIGAIFGQTEGLLGPEMNL
                     NELQRVSKVGRIEVTTTSTENTTNGNVLLPMSTDVDTCALISAAIESIDKVGPFDCSF
                     KLDAIDDVRASKKEDIVRRAKEIKQKWSTKSVSEGDTMLKDVHESIAGKVTTYGPNKL
                     PCSTGVFDSPWIILVEGRADILNLLRAGFDNALAINGATIDESIKEMCKSKKKIVAFL
                     DGDRAGGMILQELKSVVNVDIELRADSGIEVEELTPQRVADILNDTNIEMKKLGTSPP
                     TINEDDKSLATVISKIYPDLNETLESVALDDKDNQLFKIPISELVNKLSTQTGIKCLI
                     LDGIITQRLLDNAKTSGIGYIVGHRAAKLSNLGDVKIKTFTELGIS"
     gene            complement(6466..6792)
                     /gene="erpA"
                     /locus_tag="QGF01_03540"
     CDS             complement(6466..6792)
                     /gene="erpA"
                     /locus_tag="QGF01_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HJN20000.1"
                     /translation="MITITEKAAEKISEFMKEEKESPQFLRIYVQGGGCSGLSYGMGF
                     EKAAEEDDLTIEENGVKVIVDTYSQEHLKGANVDYIESLMGSGFKINNPNVTKSCSCG
                     SSFSTE"
     gene            complement(7007..7768)
                     /locus_tag="QGF01_03545"
     CDS             complement(7007..7768)
                     /locus_tag="QGF01_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJN20001.1"
                     /translation="MSLLTVKTEEGICTVKINRPEKLNAMNTDVAKEIITTFKDLDKD
                     DNVKVIILTGEGDKAFSAGADIEYMSKISAEESERYAKLGQELTATVENVTKPTIAAV
                     NGFALGGGCELAMSCDIRIASDNAKIGQPEVTIGIPPGWGGTQRLMRIVGIAKAKELV
                     FTGKAVNANVAKEIGLVNHVYEQAVLMDESLKMAKTIAANATLAVHMSKTAINKGRNA
                     DLDTGLSVELLAWRNCFSDPEREERMTNFVNKGKK"
     gene            complement(7806..9923)
                     /locus_tag="QGF01_03550"
     CDS             complement(7806..9923)
                     /locus_tag="QGF01_03550"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxypropionate--CoA ligase"
                     /protein_id="HJN20002.1"
                     /translation="MNSITKIFDDTIQTDHKVITEEAAKSILKKYGIKVPGFALVKST
                     NEAVKAAKKIGFPLVMKVVSPQILHKTDVGGVKVGIDNIADVKKTFNDMYGRLSKKKG
                     VDVKGILLEKMIPKGGVELIVGIQSNPQFGPMLMVGLGGVLTDILKDVAFRMLPITIS
                     DAKSMLNELKGSKLLKGFRGSAPIDIDMIAKALVQIGKIGVDNADYINSIDFNPVIVY
                     SKSYFVVDAKIILNKELKNNSISKAKPIITSMEKFFTPKSVALVGASATPGKIGNSVL
                     DALGKQDYKGKVYPINPKQKKILGIKCYPSLESIKAKVDLVVVCIDLSACVPIMKTCA
                     KKGIHSVVIVSGGGKELGGDRAAMEAEVKALSLKHKIRVIGPNCIGMFNAANRLDCAF
                     QGQSRMVRSKLGNVAFFSQSGTMGISMLESADLFGLSKMISFGNRSDVDEADMIWYAA
                     NDPQTKVIGLYVEGFGDGRKFIKTAKRVMKEKKKPIIIWKSGRSEAGAKQAASHTGSL
                     GGDNAMIMGAFKQAGIISVDSYQELVGVLKAMAWQPPAKGNRVAMTSNGAGPMIGGID
                     HLERLGLTIGKLTSKTRKKIKEHFPPTVPIHSGNPADVGGGATADDYRFVIQQFLDDK
                     NVDIAMPWFVFQDDPLEETIVDYLAGLSKKRKKPILCGGNGGPYTEKMIKLIEKHNVP
                     VYQDLRTWVAAASALFHWGSVRG"
     gene            complement(9965..10645)
                     /locus_tag="QGF01_03555"
     CDS             complement(9965..10645)
                     /locus_tag="QGF01_03555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HJN20003.1"
                     /translation="MTDILVIQNAAIEGIGQLGKMLESDGYTLKTINAKQEKIPQKKF
                     PFLIILGSPDSVNDNLPHFADELKLVKDYVSENIPVLGICLGSQLLAKAFGSKIYRGE
                     KKEVGFYHDIIPENQNQSKIFSNFRKPFSVFHWHNETFDLPDQATRLAYSINYPNQAF
                     KIGSAVGIQFHLEIDEPTINLWLNTTKEDISNTHIQNVKDEIPKYLTVIEENMMYFYK
                     NLKKEFSL"
     gene            complement(10725..11999)
                     /locus_tag="QGF01_03560"
     CDS             complement(10725..11999)
                     /locus_tag="QGF01_03560"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HJN20004.1"
                     /translation="MTQADPFENATKQVNDACDVLGINDEGIRDYLIMPNRFLRVKIP
                     VKMDDGKIRVFTGFRCQHNNDRGPYKGGIRYFDPEGGVEYMEREVMALSSWMTWKCAV
                     VDIPLGGGKGGIFVNPKKEKLSDGELERLTRQFASRIAEVIGPQKDIPAPDVYTTGRE
                     MTQIMDTFTKMNGNNYSPGTITGKPIPVGGSLARNVATGLGNAYCIREAAKILNIELK
                     GAKVVLQGFGNASTFSGEYLEKMGATCIAASDSKGSILVPDGFKVQELIDWKQKEGSV
                     VGYPGSKEISTEELLTTECEVLVPGALENQITAAIAEKLKCKIIGEAANGPTLPEADP
                     ILHKKGVFVIPDILANSGGVCISYLEWVQNNMGYYWSFDEVASKMETNIVKGLKDMYE
                     LSKKHNIDNRRAAMVLAVSRVLEAFNHKGIWP"
     gene            complement(12082..12456)
                     /locus_tag="QGF01_03565"
     CDS             complement(12082..12456)
                     /locus_tag="QGF01_03565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HJN20005.1"
                     /translation="MEKKLEQFHNQKYINFETYKKDGSPVRTPVWFVIDNDLLYVITK
                     EHTGKVKRLKNNQNVRIVSCSFQGEPKNEWVKGMTQLITDESANKIIKLRKKKYGFAA
                     RLIGLFTSQKGNFVVYSIQLTE"
     gene            12578..13300
                     /locus_tag="QGF01_03570"
     CDS             12578..13300
                     /locus_tag="QGF01_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HJN20006.1"
                     /translation="MMASRGYDMTPTMYSPDGRIYQVEYAIETVKRGTIAVGIQCKDG
                     VILAVEEKTRDLQVGDVTQKIFQVDDHIGVAAAGYIPDARVQVDTARFFAQSNKLTYD
                     EAVEVETVAKHLADQAHQYTQYSGVRPFGVALIVAGIDKKGTRIFVTDPSGTYVSYAA
                     VAIGGTSDDVTNFLEKNYRNDMSMDDAISLAISAINLKNDKNSTEDIKMSKITLSTQI
                     IEKVSNDELKKYAQAKNDTSAK"
     gene            13342..14031
                     /locus_tag="QGF01_03575"
     CDS             13342..14031
                     /locus_tag="QGF01_03575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJN20007.1"
                     /translation="MGLGSYWGEVLDVLQDIIPIYDKVNSYISLGKDSEHRNKAIKGR
                     IKKGDFVLDAGSGFGNMSKTAIDATNGDIHVMLYDPLVPMLKNTNRLFQKKPEVTCGV
                     FEHIPFKDEQFDAVLSGYSLRDAISLKIAISEIHRILKKDGKWVIVDLGKPDNPIIRF
                     GVSLYLKTILPIIAYVIGGKLGLKFAALYETYRLWPQNRKLESLLNEKFSKIEFEKDL
                     MGGAVMIAAYK"
     gene            14079..14432
                     /locus_tag="QGF01_03580"
     CDS             14079..14432
                     /locus_tag="QGF01_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HJN20008.1"
                     /translation="MIEYSLNIIGGEWLIIIFAALILLLGTNRFPETAKKIGKIVGEY
                     KKAKDTVEKQMKDVTKENFEISGPVQNERQKLDIMSKTLGIDSKNTNDEDLRKIIQNK
                     IGHSENDTVVKNKNN"
     gene            complement(14440..15210)
                     /locus_tag="QGF01_03585"
     CDS             complement(14440..15210)
                     /locus_tag="QGF01_03585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HJN20009.1"
                     /translation="MKKFSLNKIYNQNCIDGMKDIPDNKIDLVITDPPFAINFKPKKA
                     NYNRIASRVLPGYNEIESKDYYNFTYNWMSQINRILKKSGSMYVFSGWNNLKDILTAL
                     DENGFITINHIIWKYQFGVVTSKKFVTSHYHCLYVCKNNNERKFFPYSRFDKNAKTTD
                     GKSLHYSDKEDVWNIKREYWTGDEKTPTKLPAEIIKKILEYSSEEKDVIFDPFLGSGQ
                     VAVVSKMLKRKYLGFEIVKEYYNFAKKRLEKNVYRLKK"
     gene            complement(15282..15476)
                     /locus_tag="QGF01_03590"
     CDS             complement(15282..15476)
                     /locus_tag="QGF01_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20010.1"
                     /translation="MASYTTEVASIHRQFQAGLKKANSRQAVLNCYWKHKAQHERLLK
                     KHLKEEMAQVNRIKSKIKYR"
     gene            15617..16450
                     /locus_tag="QGF01_03595"
     CDS             15617..16450
                     /locus_tag="QGF01_03595"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJN20011.1"
                     /translation="MEKIAVVTGTSTGIGFETALALAREGYYTYATMRDITKGQKIKE
                     LGQKENLKINVLKLDVDDENSVKTAIQKILDEKHRIDVLVNNAGWALWGCVEDVSVDE
                     FKAQFDTNFFSIIRLIQKVGPTMRKQGSGTIVNVSSVVGRIGFPASPAYISSKFALEG
                     LSESLRFELAPFGVNVVIIEPGVIKTNFMKNMKMAKKYGPESVYTDITDKVVSGIKMM
                     CEMGTDPKEVADAIVKATKDENPLPRYIVGNDASMFLEAKKNKTDLEFENYIKKELYG
                     E"
     gene            16560..18251
                     /locus_tag="QGF01_03600"
     CDS             16560..18251
                     /locus_tag="QGF01_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HJN20012.1"
                     /translation="MKWKTLEHKGILFPPEYESVGIKIKINGENVSLTLEQEEMVYQW
                     AKKKDAPKPGATEKYVEDPVFQKNFIKDFAKTFNNKFKDLKYADIDFTQSYKLVDKEK
                     EVKELMTKEEKKALAVERKKIRENMKISYGKAIMDGKEVDIANYMAEPPGIFMGRGEH
                     PMRGRFKPRVTAKNVTLNLGKDAKIPDGKWGKIVHDKDSMWIASWMDILTQKRKYVWL
                     ADTAGIKQERDQAKYDKAKKLAKEIESIKTQISKDMQNKDPRTRKISTACYLIYRTAM
                     RVGDEKDPDEADTVGATTLRKEHIKLSGNAIEFDFLGKDSVRWKETIPAEGQDKQFHD
                     NLKELVSNKKNNQEIFDDITSRHVNVYYSTIVKGLTAKVFRTYLASSVVSKYLRAHDN
                     IKSESNMKKLFHAKLANLDAAKMCNHKRTIPKNFDQTLEKKKNTLNDVEKAKPWQKYE
                     ESLKKTQSKIIKTEVQRETQKKQIRKIKTMIKKKKEKHTERIEKLQLQIDLTQKTRDY
                     NLGTSLRNYIDPRIFQSWTDEVVAEWEKLYTSALQKKFLWVKNVDSKWSEISKYY"
     gene            18294..18389
                     /locus_tag="QGF01_03605"
     tRNA            join(18294..18326,18347..18389)
                     /locus_tag="QGF01_03605"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18350..18352,aa:Met,seq:cat)
     gene            18468..19658
                     /locus_tag="QGF01_03610"
     CDS             18468..19658
                     /locus_tag="QGF01_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20013.1"
                     /translation="MSELDEFAEALMGQISVELDEEKEIATLSKKINDDKDFTIKFDD
                     VKSIVQKIFPDLKQKVYDYTGLEISNELSIEYLGLDGFKKLKGKKVFTDNAREFIDKL
                     FDAVTKNDLKKIAEIIGEDTAKFLVYSTYVKSYISKLTTTYGDYLDSKIYLNMFILGD
                     YPKIILYKQGPPYQMKSESVKSGYLGALKMTVLEEIIHSVQTNLQKLNIQAVVQVNTI
                     NEELAKTILGLDEKTVTELTEYLQLQLVPEEFKIAKKANLFFMLNPDNFITNVMGPDV
                     MTYTRVEIDPKISDFIPSLEAIYQRWLKPIQSQHAIFTTMEGMAEFLVQRILKDDTDF
                     QNYLTTFVGTDYSAYSVKKSTGKEFTEYLFNEFGKNTFEKLIIDPPNTKELKNPQLYL
                     NRVG"
     gene            19670..20497
                     /locus_tag="QGF01_03615"
     CDS             19670..20497
                     /locus_tag="QGF01_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HJN20014.1"
                     /translation="MTTEKFDPFVTEWLSFSQDPKYNLIEKCLKLAQILDYPELNITD
                     YIEKINQIGNSLKLKIDDVENPIYLISMLSEHIFENYGFSGDDQNYYDPRNNFLNTVI
                     DKKSGIPITLSIVYTEIAKYIGLDLKIVGFPGHVIVKYNDDIILDPFYRGRLLTIENL
                     EEILHSNFGEDVEMISEYLDEATEEQVLTRLLRNLKNAYTQSFAYDKAMKCANMILGI
                     QPDSPEEIRDKGILEERLLHYDKALPLLNKYLEMQPEAEDVDFILELIKNVREKSNQ"
     gene            complement(20487..20666)
                     /locus_tag="QGF01_03620"
     CDS             complement(20487..20666)
                     /locus_tag="QGF01_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20015.1"
                     /translation="MPKQTDESETIYERVAKLEDEVASLRNEIDFLKNAFKNKLSRHE
                     IKMVRKGNDVSSIID"
     gene            complement(20722..20883)
                     /locus_tag="QGF01_03625"
     CDS             complement(20722..20883)
                     /locus_tag="QGF01_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20016.1"
                     /translation="MGRRKTQKIVKSGPKNVTTGMCPECQTIGRIFIIGQVGKERGKC
                     QSCNQEFEL"
     gene            complement(20924..21007)
                     /locus_tag="QGF01_03630"
     tRNA            complement(20924..21007)
                     /locus_tag="QGF01_03630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20971..20973),aa:Ser,seq:gct)
     gene            complement(21106..21852)
                     /locus_tag="QGF01_03635"
     CDS             complement(21106..21852)
                     /locus_tag="QGF01_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HJN20017.1"
                     /translation="MEIVQLSDLHIGAQFREEVFGNVIDEINSLKPEAVVITGDLTNE
                     GLIEQYEKCKNLVSQIKTEKIIAISGNHDYRNTGYLLFKKYFPFKAENPIGNDVILVT
                     LGSARPDRDEGEVGHHQNLWLERTLKKYENKLKIVAMHHHLIGIPDTGSDRLTVIDAG
                     DVLRTILDSNVNLVLCGHKHRPWIWDFNTLSIANAGTASSERVRGFFENSYNIVNVDN
                     GTFRVDLKIVNGKRTPLRNIVQDYTQTSEN"
     gene            21961..22995
                     /locus_tag="QGF01_03640"
     CDS             21961..22995
                     /locus_tag="QGF01_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HJN20018.1"
                     /translation="MTKKAVVMKGDGIGPEVVDSMLKVLKECNLQSELIHCEVGSEQW
                     ERNGFKEKSYIPDKTMEALDDADACFKGPTTTIPKPDAPKSVAVTLRQKFELYSNIRP
                     IKTFKRLTPNRNLDFVCFREATEGLYSGIEVQISDDAAIAIRQITRKNCRRFILSGFQ
                     WAKKYNLKKMTGITKRNILKKTDGIFWEELENCQKEFPNMELEEIYIDNMSQQLVINP
                     EQFNNSVLLSTNLFMDIISELASGIVGSIGLIYSANMGDSFAMFEAAHGSAPSIAGQN
                     KANPTATILAGAWMAEYLGESDIKNAIFSATEQVINENKATTSDIGGSAGTTQMTDAI
                     VVYAKQKLRR"
     gene            complement(22984..23526)
                     /locus_tag="QGF01_03645"
     CDS             complement(22984..23526)
                     /locus_tag="QGF01_03645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HJN20019.1"
                     /translation="MQKKLLEIEPYPPAEDTFFLADYIEKISGKSVLDIGTGSGYLAD
                     LLSRSFPLVVATDLSFFTLKKQNMFSNNIICCNGADALNYKFEFVICNLPYLDTNEIL
                     DIRTDGGKDGLEIPKKIIDSVQSCVIPGGKLIYVTSSLSNFQKLIDYTELKGFRAQIA
                     AKKKLFFEELILVEATKLPS"
     gene            complement(23501..24202)
                     /locus_tag="QGF01_03650"
     CDS             complement(23501..24202)
                     /locus_tag="QGF01_03650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HJN20020.1"
                     /translation="MKKRQNFGQHFLKSNTIANHIVSEAKITKNDVVFEIGTGHGILL
                     PYMCKKANKIISIEIDKNLYNLAKSKFHDEKNLTLEYGDGFKSNHSFTIFISNLPYSK
                     SRKSIEWLLQKNFSRAVIMVQKEFADKLLSSKNHTAISILTNYGFQLKIIMDVKKSNF
                     YPVPKVDSVVIFLTQKKIVSKTIISTVNRMFSYRRKTLQNVLKQFGINSTSKERLDDL
                     SGDEIIKIAKKIIRN"
     gene            complement(24199..24759)
                     /locus_tag="QGF01_03655"
     CDS             complement(24199..24759)
                     /locus_tag="QGF01_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HJN20021.1"
                     /translation="MGNTQPRRYEEYAHVLDFKPRGKSTTVKGRTGNIIIAIGDERLT
                     LLEILGLEDTTFEIGEKIYIGKEGRTKVQSVLGKLDYAKISVTAQSEISGIVELIIKE
                     NEKRFVDYLNKAQPLTPRIHALELIPGIGKTYLNLILDEREKKLFESYHDFEERVGLK
                     DLITNISKRIHEEISGKARMNLFVKR"
     gene            complement(24780..25106)
                     /locus_tag="QGF01_03660"
     CDS             complement(24780..25106)
                     /locus_tag="QGF01_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4"
                     /protein_id="HJN20022.1"
                     /translation="MEEVKKKQLISLAEVKEILGKVDAESLDQIQRWTYDYVSKFSNV
                     DPKIAKKMKQDLIKECGLTEEEAVEILNIMPESLPELRSFTFGWKKLILSETLEKTLK
                     IIKEHS"
     gene            complement(25109..25408)
                     /locus_tag="QGF01_03665"
     CDS             complement(25109..25408)
                     /locus_tag="QGF01_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HJN20023.1"
                     /translation="MTTKKPGGRSHGFKHKSRSVMTKDAPRGVSFLLHEYHDGDKALV
                     IIDPRQHKGLPHRRYHGKVGTVSKVGRRSVVLDIKLGNKSKTLITRFDHIKPFGV"
     gene            complement(25449..25883)
                     /locus_tag="QGF01_03670"
     CDS             complement(25449..25883)
                     /locus_tag="QGF01_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20024.1"
                     /translation="MSDKYSVSKAKMFQVAAATHRITQDPDVKNLEMFEVVFTMIFAK
                     PMDASHIVVETENKFGIPEILYLTSALKVNENLPQALTLEEKSKFEIEYEQEVIPEII
                     MDQEPDMDKVTCGDGTILKDNLCVPKEWSFLDFFEKLMKLFG"
BASE COUNT         8874 a         4338 c         4435 g         8615 t
ORIGIN      
        1 tatacatcta aaagataagt taagaatttc tagagaagaa gcgttggata gggcagtaaa
       61 aactgttgaa tatgctaaat ctcatggtct gaaaataagg ttcacagtag aagatggatg
      121 tagggctgat ccacagttcc tcattaaggt atgtaaagca atagaagaag ctggtgtgga
      181 taggattagt cttcctgata ctgttggagt cttacgacct ataggaatgt ataattttgt
      241 taaaagagtt cgttctgagg tgaatgtgcc attagatgca catgttcaca atgatattgg
      301 gtttgcactt gcaaatgcat ttgccgcatg tgatgctggt gctgatcaaa tacacacaac
      361 aattgatggc attggtgaaa ggacaggaat tccatctctt gcagaaactg cagtagcact
      421 tgcatatctg tataaatcac caaatgattt tagattagac atgctggttg atctttcaag
      481 actaattgaa cagtatacaa caataaaacc atatgactca aaaccaattg ttggggaatc
      541 agcttacaaa cataaagctg gaacacattt ggctgcaatt ttgagaaacc ctgctgcgta
      601 tgagccaatt ccaccaagaa ctgtaggcca aacacgaaaa gcagttttcg gagaattagc
      661 tggaaaaact ggtgcagaat acctcatgtc catattagga ttagaaaaaa atgtcgaaaa
      721 cgcaaaaaat gttgcaaatg gcttaaagaa tcttagaatg ggtgatctga tcgaagttcc
      781 acttgaaaat aatgtagaaa gaaagattaa tgaagaaaaa atgagtaaag gtaggaaaaa
      841 tgactaactg ttctgaatgt gatgcagatt tgtcagtacc aagtgacgca attgaaggag
      901 aaatcgtgac atgcgctgag tgtggggcga gttttgagct agtaaaggct cccaatggat
      961 ttgagttaaa accagcccaa actgttggag aggactgggg acagtgacta gtatcacaat
     1021 tctttatgat acaatacgtc ttgaagagaa attattgatt gaatctgcaa agaaaaataa
     1081 tataacaata gagatggttg attgtaagaa attgtttata gatctagata aaaattcaca
     1141 tgatatacag actgttttac agcgatgtgt tagttattat cgtaatattc attctactgc
     1201 ggtattagaa gggcaaggtg taaaagtcgt caattgttta aacacaggta tttttgcagg
     1261 aaataaactc tttacgcata tgctattaaa aaaggcagga attgaaacac caaatgcaac
     1321 tgtagctttt tcgaaggatg cagcaatgaa aatgtttggt gagtgtggtt ttccaaaaat
     1381 cataaaacca actgttggta gttggggtag gatggtatca aaaattaatg atatggattc
     1441 cgcagatgga attatagaag gtaaggaagc tatgcaccca attcatcata tacatttttt
     1501 tgaagaattc gttcaacgtc ctccaaggga tattcgtgta attgtgattg gtgataatgc
     1561 tgttgctgca atttatagaa attcagcaga tggtaactgg aaaactaata cccatcttgg
     1621 tggctccgct gaaacatgta agattacaaa tgaattagaa gatgtttgtt taaaggcaaa
     1681 aaatacagtg ttcggtgata ttgtgggtgt agatttgatg gaaagcgaag aaaagggtct
     1741 tgttgttcat gaaataaaca atacaacaga atttagaaat gttgttagag tgacaggaat
     1801 agatgtccca aaattaatgt tagagcatgt aaaagggttg caataaaatg gatactcatt
     1861 tagtttcttc aaaatatgct gtaaagacac ttgaaaaggc gttaagaatc tacacacctt
     1921 cattacggga aaaagcatta gctgattttc ttgcagatag atgtgatgat ttaggattta
     1981 aagacataca tcaagatgaa gttggaaatt tgattgccat aaagggttca ggtgaaccaa
     2041 gaattatgtt atgtggccat atggatgtag tcccaggaag aatcaaagtc agaaaagaag
     2101 gagattattt atttggtaga ggagcgtcag atgcaaaatc tccattaatt gcgatgttat
     2161 ttgcggcatc tagtatgcaa gaaaatgaag gtacggttat ttttgtgggt gcggttgatg
     2221 aagaaggcaa tgcaacagga attaaaaacc ttgtaaaaaa taaaccagag atagactatg
     2281 caatttttgg agagccaagc agtattcaga aagtaaccat agcatacaag ggaagattag
     2341 cgataaatct caagattagt gttgaaaaca gtgctcatgc tagtgcgcct tggctagcaa
     2401 aaaatgcaat cgaagaaaca gcaaccgtag tcaaagattt gaaaaataat ctggaaggtg
     2461 gacaggaaaa taaaacaaaa ggaatgatgc ttactgcatc attaacggaa attcgtggtg
     2521 gaacaagcca taatattaca ccaaaggaat gcattgcaac catagatatc agaattccag
     2581 tagacatgaa ttgtaacagt gtggaggaaa aaattacaac tagtataaat gaaatttctt
     2641 cgaaacgtgg aattgagatt ttatattcgg ttgttgatga aactgaacca tttgaggctg
     2701 ctcataattc agcattagtt agagctatca cacttgctgt attggatgta gaacaaaaaa
     2761 gacctacatt aattagaaaa actggaactg gtgacatgaa tgtagttggt aaccaattaa
     2821 aaatcccagt tattacatat ggacctggtg atcctcatga agcacataca attgatgaaa
     2881 aagtatccat agaagaattc ctaaaaggaa ttgatgtgtt caaaaggaca ttatttcatt
     2941 taaaaagatt gcatggtaga agtaaataat gctgtggttc attggtcttg gtatatctgg
     3001 aactgagtca atacctagtg aatccataaa aattattgag aaagctgatt tagtatattt
     3061 ggaatcattt acgagtccaa tttacgtgaa acatgaagaa gaaattaaaa acttagtgca
     3121 tggtgaattc aaaattgcaa aaagatggat ggtggaggat ggtaaggaaa tacttaatgc
     3181 ttcaagaagt tctattgtag ttttattatc ttatggggat ccgtatatag ctacaacaca
     3241 catagaacta agaactagag caagattaga agatatagaa acaaaaacaa ttcattctac
     3301 ctcagcaata actgcaatga taggagagat aggattacaa ttctataaag ttggaaaaat
     3361 tgtaactata atgagtgaca aaaaatctgt aacaacacct tatactacta tttttaagaa
     3421 tctaatagaa ggattacatt ctataatatt attagaatat aatgaggatc aaaatttttt
     3481 tcttgaccct aagactgcta ttttgagcct gctagacatt gaaaaggaac aaaaacgaaa
     3541 agttgtaagt aatgatacat ttgcaattgt tacttcaaga gttggaatga aaacacaaaa
     3601 aatcacctcc ggaaaattca gtgatctgct caaaatggat tttggtgaac cgccacatag
     3661 tgttattatt actggaaaac tacattttac agaatctgat gctatcagcg cattaactga
     3721 ttgtttatcc aagccagccg ataattcaaa tgatattaag aatatctcaa tgcagatgat
     3781 tgaaaaatat gttccaatgg ttagagaagc attaaatgaa attaaaccat tttatagtga
     3841 tgcaaaagag tttcaggata tacttcaaaa tgcagaactt tacattgatg atgcagaaaa
     3901 ttttctaaaa gaaggaaagg atgaaaatgc agttttgagt attggttatg cggatggttt
     3961 agtagatgct ctaagaattg caaagggtat agatccaaaa atgtagatta tcgatgcgtt
     4021 aaaaagtaat aagaagagtg aggaatataa ttggattttt cagtaaagtt gagaaatgat
     4081 tgggaaaaac gtggactcaa aattgtgttg gctggaggag tttttgatat tatccatcct
     4141 ggtcatatac acacccttaa tgccgcaaaa gatctaggcg atgtcttagt agttgtaatt
     4201 gctactgaca aaacagccat aaaaatgaaa aaaagatcac cactacatag tcaggagtct
     4261 agatgtgaat tggtaagttg tttatccatg gttgatgttg taattatcgg taatgaagat
     4321 aatatctttg aaactgtaaa actagtaaaa cctgatgtaa tcgcattagg atatgatcag
     4381 atccatcaag aaaaattcat atctgatgga tgtaagcaaa ttaaccttga tgtggaaata
     4441 gcaagattgt cgtcacccat tcctgaatta tcaagctctg acattcaaaa aaaatatggt
     4501 gaagatatcc atgatcttta gtttacgatc gattaggaat taaaatttta attgtgactt
     4561 tagcgccagt ttttagttta gcagttttac gtaaattatt ttttgatatt aattcaatca
     4621 ctgaattatc atgatgtgtt ctctcaagac gaattaattc acatttagta gaattattta
     4681 atttggcatg aaaacatttt acccatccat atgtcctctt tccatcagaa aatggttcaa
     4741 tcattatacc atctaaaaca atgaattgtt caactgcttg gatatattct ggctgattta
     4801 attttacatt aagtgttcct ggaaatggga taaatccaat ttttgatttg aattgttttg
     4861 tatatccctt caatgacata taatatgcac cctctcccat ccctgaaatt attgtaccct
     4921 ttaattctag agaagataca gatgtttcta tattagcacg taaaagtaga tgtagtttaa
     4981 gtaattcagc atatccattt tgggtaattt taacagaaac ttttcttcca tgaactactc
     5041 gttcaatgaa tccattcgtt tctaattcta aaagatgttt tgaggcagat tgctgcgatt
     5101 tttttatatt tttacctaga gatgatgtgg tgatttgaat aaaattatac cgtgcaccct
     5161 tagacagtaa ttgtgataat gtgagaatat gttgaatctt tagatcagac ataaagaatt
     5221 aagatatgcc gagttctgta aatgttttta tttttacatc accaagattt gataactttg
     5281 cagctctatg accgactata tacccaatac cagatgtttt tgcattgtct aataatctct
     5341 gagtgattat tccatccaaa ataagacatt ttataccagt ttgcgtagac agtttattta
     5401 ctaattcact gataggtatt ttgaataact gattgtcctt atcatctaat gcaacagact
     5461 ctagtgtctc atttagatct gggtaaattt tacttattac agtggctaat gatttatcgt
     5521 cttcgtttat tgtaggtgga ctagtaccta attttttcat ttcaatgtta gtatcattta
     5581 ggatatctgc tacacgttga ggggtaagct cttccacctc aatccctgaa tcagcacgta
     5641 gttcaatgtc tacattaaca actgatttga gttcttgtag aatcattcca ccagctctat
     5701 caccgtccaa aaatgccaca attttctttt tggatttaca catttcttta atagattcat
     5761 ctatagttgc accatttatt gctaaagcat tatcaaatcc agctctaagt aagtttaaga
     5821 tatcagccct tccctccacc aaaattatcc atggtgaatc aaaaactcct gtactacatg
     5881 gaagtttatt tggaccgtac gtggtaactt ttcctgctat gctttcatgt acatctttca
     5941 gcatggtatc cccctcacta acagatttag tggaccattt ctgtttgatt tcttttgctc
     6001 tgcgtacaat gtcttccttt tttgatgcac gtacatcatc tattgcatct aatttgaaac
     6061 tgcaatcaaa cggaccaact ttatctatac tttcaattgc agcagatatt agcgcacagg
     6121 tatcaacatc agtactcata ggtagaagta cgttaccatt tgtagtattc tcagtagaag
     6181 tagtagtaac ttctattctt ccaaccttag aaacacgctg aagctcattc agattcatct
     6241 cagggcctag aagaccctca gtttgtccga atatagcccc tatgatgtct gcacgttcga
     6301 caagaccatc gacatcgaat gatagtttaa catgatactt gacgattcca gtgtacggca
     6361 attaattttc atctccattt tattcaatat atttaagtct tcaatgatgg ttataagtgc
     6421 ttgcggatca aggataactt tctataataa agtttgaaaa aatgattatt cagtactgaa
     6481 cgatgaacca caagagcatg atttggtaac attaggatta ttgattttga atccagatcc
     6541 catgagactt tcaatgtagt ccacatttgc acccttgaga tgttcctgac tgtaagtgtc
     6601 aactattact ttgacaccat tttcttcaat agttagatca tcttcttctg ctgctttctc
     6661 gaaacccatt ccgtaagaaa gtccagaaca accaccgcct tgaacatata ttctaaggaa
     6721 ctgtgggctc tccttttctt ctttcatgaa ttctgaaatt ttttctgctg ctttctcagt
     6781 gattgtgatc aattttcctg attgtgtttg aggttgtggt gactcgacaa ctacttcttg
     6841 aggttttgga gattcatgag attctccatg atcgtgtcca cacccacatg aatgatcagc
     6901 agatgtttcg tgactttgtt ctgtagtcat atattcaact ctggttggtt caaattataa
     6961 taaccttttc ctattaaaca tattaggaat aaaataagaa gtctgtttat tttttaccct
     7021 tattaacaaa attagtcatc ctttcctctc tttcaggatc tgaaaaacaa tttctccaag
     7081 caagtaattc aacactcaat ccagtatcca agtctgcatt tcgtcctttg tttattgcag
     7141 tttttgacat atggacagca agagtagcgt ttgcagctat tgtttttgcc atttttagag
     7201 attcatccat taagactgct tgttcatata catggttaac aagaccgatc tcttttgcaa
     7261 catttgcatt aactgcttta ccagtaaaaa caagttcttt tgcttttgca attcctacaa
     7321 ttctcattaa tctttgtgtt cctccccaac caggtggtat tccaatcgtt acttctggtt
     7381 gaccaatttt tgcattatct gatgcaattc taatatcaca agacatagca agttcacatc
     7441 caccacccaa agcaaatcca ttaactgcag caatagttgg ttttgttaca ttttcaacag
     7501 tagctgtaag ttcttgtcca agttttgcat aacgttcaga ttcttctgct gatatttttg
     7561 acatatattc tatatcagcc cccgcagaaa atgctttatc accttcacca gttaaaataa
     7621 ttactttaac attatcatct ttgtctagat ccttgaaggt agtaattatt tccttagcaa
     7681 catcagtgtt cattgcattt aatttttcag gacggttaat cttaacagtg catattcctt
     7741 cttcggtttt tactgttaag agagacataa agcaaatact tccatgatga aattaaatgt
     7801 tatctctaac ctctaacgga accccagtgg aataatgcag atgcggcagc aacccacgtt
     7861 cgaaggtcct ggtaaactgg gacattgtgt ttttcaatca attttatcat tttttcagta
     7921 tatggaccac catttccacc acataaaatt ggcttttttc ttttctttga aagtccagct
     7981 aaataatcaa caattgtttc ttcaagagga tcatcttgga atacaaacca aggcattgca
     8041 atgtcaacat tcttgtcatc taagaattgt tgaataacga atctataatc atccgcagta
     8101 gcaccaccac ctacatctgc tggattacca ctatgaatag gaactgttgg tggaaaatgc
     8161 tcctttattt ttttgcgagt ttttgatgtt aactttccta tagtaagacc caatctttct
     8221 aaatggtcaa ttccaccaat cattggaccg gcaccattac tagtcatggc aacacgatta
     8281 ccctttgcag gaggttgcca cgccattgcc ttcaagacac caactaattc ttgataacta
     8341 tcaacagaaa taattcctgc ttgcttgaat gcacccataa tcattgcatt atcaccaccg
     8401 agagaacctg tgtgtgatgc ggcttgtttt gcacctgctt cgcttctacc actcttccaa
     8461 attataattg gtttcttctt ttctttcatt acacgttttg ctgtttttat gaattttctg
     8521 ccatcgccga atccttcaac ataaagtcca attactttag tttgaggatc atttgcagca
     8581 taccatatca tatcagcttc atcaacatca gaacggttac caaaactaat catttttgat
     8641 aaaccaaaca aatcagcact ctccaacatg ctaattccca tcgttccact ttgtgagaag
     8701 aatgcaacat ttcctaattt tgaacgaacc attcttgatt gtccttgaaa tgcacaatca
     8761 agtctatttg ctgcattaaa cattccaata cagttaggac cgattacacg aattttatgt
     8821 tttaatgata atgcttttac ctctgcctcc atagctgctc tatcaccacc aagctcttta
     8881 cctccacccg aaactattac aacactgtga attcctttct ttgcacaagt tttcatgatt
     8941 ggaacacatg ccgaaagatc aatacaaaca acaaccagat caacttttgc cttgattgat
     9001 tctaatgaag gatagcattt gattccaaga attttctttt gtttaggatt gattggatat
     9061 actttacctt tgtaatcttg tttacccaac gcatctaaga cagaattacc aatttttcct
     9121 ggtgttgcag atgcaccaac taatgcaact gattttggag taaagaattt ttccattgat
     9181 gtaatgattg gttttgcttt tgaaattgaa ttatttttca gttctttatt tagaatgatc
     9241 tttgcatcta ctacaaagta agattttgaa tatacaatta cgggattaaa gtcaatactg
     9301 tttatgtaat cggcattatc gactcctatc tttccaattt ggactaatgc ttttgcaatc
     9361 atgtctatat caattggtgc actaccacgg aatcctttga gaagttttga gccttttaat
     9421 tcatttagca ttgattttgc atcagatatt gtaattggaa gcattctaaa tgcaacatct
     9481 ttaagaatat cagtaagaac accacctaaa ccaaccatga gcattggacc aaattgtgga
     9541 ttgctttgta taccaacaat tagttcaact ccaccttttg ggatcatctt ttccaaaaga
     9601 attcctttta catccacacc ttttttcttg gataaacgac catacatatc attaaatgtc
     9661 tttttgacat ctgcaatatt gtctattcca acttttacac caccaacatc agttttgtgg
     9721 agaatttgtg gagatacaac tttcattact aatggaaatc caattttttt tgcagccttt
     9781 actgcttcgt tggtagattt tactagagca aacccaggaa ctttaattcc atactttttt
     9841 aaaattgatt ttgctgcttc ttcagttatt actttatgat cagtttgaat tgtatcatca
     9901 aaaatttttg taatactgtt cataatagat cgataaaaaa atttttcaat atattaaact
     9961 ttggtcataa agaaaactct tttttcagat ttttataaaa atacatcata ttttcctcga
    10021 tcacagtaag atattttggt atctcgtctt tcacattttg gatatgtgta ttggaaatat
    10081 cttctttggt ggtatttagc caaagattga ttgttggttc gtcaatctcc aaatgaaatt
    10141 gaatcccaac tgcactacct attttgaaag cctgatttgg ataatttatt gaatatgcta
    10201 atctagtggc ctgatctggc agatcaaacg tttcattatg ccaatgaaaa actgaaaaag
    10261 gttttctaaa attagaaaat atttttgact ggttttggtt ttctggaata atatcatgat
    10321 aaaacccaac ttcttttttt tctccgcgat aaattttaga tccaaacgct tttgctaata
    10381 attgagagcc taaacaaatt cccagcactg gaatgttttc tgaaacatag tctttcacaa
    10441 gttttagttc atctgcaaaa tgtggtagat tatcatttac actgtcgggt gaacctaata
    10501 ttataagaaa aggaaacttt ttttgaggta ttttttcctg ttttgcattg attgttttga
    10561 gtgtataacc atcactttct aacatcttgc ctaattgacc aattccttca atggctgcat
    10621 tctgaataac aagaatatca gtcatgttac aaatttttta aaacatatta tatgtttagt
    10681 agatgaatga tcaagaatat tcagagagaa taatgcattt acaattatgg ccagatgcct
    10741 ttatggttaa atgcttctaa gacacgactt acagccaata ccatagcggc tcttctattg
    10801 tcaatattat gttttttaga aagttcatac atatccttca gacctttgac aatgttagtt
    10861 tccatcttac ttgcaacttc atcaaatgac cagtagtaac ccatgttgtt ttgcacccat
    10921 tccagataag agatacaaac accaccagaa tttgccaaga tgtccggtat aacaaaaact
    10981 cctttcttgt gaagaattgg atcagcctca ggtaaagtag gaccgtttgc agcttcgcca
    11041 ataattttac attttaattt ctctgcaatt gctgcagtga tttgattctc aagtgcaccg
    11101 ggaaccaaga cttcacattc tgttgtaagc aattcctctg tagaaatctc tttactacct
    11161 ggataaccaa ccacagatcc ttccttttgc ttccaatcaa ttaattcctg aactttaaat
    11221 ccatctggaa caagaattga accctttgaa tcacttgccg caatacatgt tgcacccatc
    11281 ttttctagat attcaccaga aaaggttgaa gcgtttccaa atccttgcag tacaacctta
    11341 gcgcccttaa gttcaatatt caaaattttt gctgcttctc taatacagta ggcattgcct
    11401 aaacctgtag caacatttcg tgcaagagaa ccacccacag gaattggttt tccagtaata
    11461 gttcccggag aataattatt tccattcatt tttgtaaatg tgtccataat ctgagtcatt
    11521 tctcttccag ttgtgtaaac atctggagca ggaatatcct tctgtggacc aatcacttca
    11581 gcaattctag atgcaaattg tcgagttaat ctttcaagtt caccatcact aagtttttct
    11641 ttttttggat taacaaaaat tcctccctta ccgccaccta gtggaatatc aacaacagca
    11701 cacttccatg tcatccaaga agaaagagcc attacttctc gttccatgta ttcgacacca
    11761 ccctcaggat caaagtatct tataccgccc ttgtatggtc ctctatcgtt gttatgctga
    11821 catctaaatc ctgtaaagac cctaatctta ccatcatcca tttttactgg aattttgact
    11881 cgaagaaacc tatttggcat gatcaaataa tctcttatgc cctcatcgtt tatccctaaa
    11941 acatcacatg cgtcattaac ctgttttgtt gcattctcaa atgggtcagc ttgagtcaag
    12001 atggatcaga tttaattcaa atttgtattt aaatgtctct agtaaaatca gatttttaaa
    12061 aaataataga tggtaaaaat tctactcagt taactgaata gaatatacaa caaaattacc
    12121 cttttgagat gtgaataaac caattaatct tgcagcaaaa ccatattttt tcttacgaag
    12181 tttgataatt ttatttgcac tttcgtctgt aatcaattgt gtcatacctt tgacccattc
    12241 atttttaggc tctccctgaa aggagcagct aacaattcta acattttggt tatttttgag
    12301 tctcttaact ttgcccgtat gctctttagt tattacatat aatagatcat tatcaatgac
    12361 gaaccaaaca ggtgttctaa caggcgatcc atctttcttg tatgtttcaa aattgatata
    12421 tttttgatta tgaaattgct ctaatttttt ttccatatta tgtatagagt agttctgtat
    12481 tttaacacac tcaaacagat aagaatatga aagtgaataa aaatttcaat tcagaataac
    12541 taacccttaa attgacttcc attttaattc agatattatg atggcatcac gtggctatga
    12601 catgacacct acaatgtatt caccggatgg tcgaatttat caggttgaat atgctattga
    12661 aactgtaaaa agaggaacca tagcagttgg tatacaatgt aaggatggtg tgatacttgc
    12721 agtggaggaa aaaactcgtg atcttcaagt gggagatgta acccaaaaaa tttttcaggt
    12781 tgatgaccat ataggtgtag cagcagcagg gtatattcct gatgcacgtg ttcaggttga
    12841 cacagcacga ttttttgccc aaagtaataa actcacttat gatgaggctg ttgaagtgga
    12901 aacagttgca aaacatttag cggatcaagc tcatcaatac acacaatatt caggtgttcg
    12961 tcctttcggt gttgcactaa ttgttgctgg gattgataaa aagggaacta gaatatttgt
    13021 tactgatcct agtggtacat atgtttcata tgctgcagtt gcaattggtg gtacatcaga
    13081 tgatgttacc aattttttgg aaaagaatta tagaaatgat atgtctatgg atgatgccat
    13141 atcattagca atttctgcta taaatctcaa gaatgacaaa aacagtacag aagacatcaa
    13201 aatgtcaaag atcacattga gcacacaaat catagaaaaa gtctctaatg atgaattgaa
    13261 aaaatatgct caagcgaaaa atgatacatc tgcaaagtag tatatcatat aaaatataat
    13321 atcagatatt gtagaattaa tgtgggttta ggaagttatt ggggagaagt actagatgtt
    13381 ctgcaggata ttattcctat atatgataaa gtcaattcgt atatttcatt aggaaaggat
    13441 tctgaacatc gtaataaggc cataaaaggc agaataaaaa aaggtgattt tgtacttgat
    13501 gctggttcag gatttggtaa tatgtcaaaa actgcaatag atgcaaccaa tggtgatatt
    13561 catgtgatgt tatatgatcc attagtacca atgttaaaaa atacaaatag attatttcag
    13621 aaaaaacctg aagtgacttg cggtgttttt gagcatatac catttaaaga tgaacaattt
    13681 gacgcagttc tttctggtta ttcattacgt gatgcaatta gcttgaaaat agcaatttca
    13741 gaaattcata gaattttaaa aaaagatggc aagtgggtta tagttgactt gggtaagccg
    13801 gacaatccaa tcattaggtt tggtgtttca ctatatttga aaacaatttt gccaattatt
    13861 gcgtatgtaa ttggaggaaa acttggatta aaatttgcgg cactttatga aacatataga
    13921 ctctggccac aaaatagaaa actagaatca ctcctgaatg aaaaattttc aaagattgaa
    13981 tttgaaaagg acctcatggg tggtgcagtg atgattgctg catataagta aaattcaaaa
    14041 atccaaatct ttaattttat actattaaga ataatttcat gatagaatat tcgttaaata
    14101 tcataggtgg tgaatggtta ataattattt ttgctgcatt aattttactg ctgggcacaa
    14161 acagatttcc tgaaactgca aaaaaaatag gaaaaattgt gggtgaatat aaaaaagcaa
    14221 aagatacggt tgaaaaacaa atgaaagatg tgacaaagga gaatttcgag atatcaggac
    14281 ctgtacaaaa tgaaagacaa aagttggata taatgtctaa aactttggga atagattcca
    14341 aaaatacaaa tgatgaagat cttagaaaaa taatacaaaa taaaattgga cactctgaaa
    14401 atgatacagt ggtaaaaaac aaaaataact aagtttctgt tatttcttca atctgtaaac
    14461 atttttttca agtcgttttt ttgcaaaatt ataatattct tttacaattt caaatcctaa
    14521 gtattttctt ttcaacattt tactaactac tgcaacctga cctgaaccta aaaatggatc
    14581 aaatattaca tctttttctt cacttgaata ttccaatatt ttttttatta tttcagcagg
    14641 taatttagtt ggagtttttt catctcctgt ccaatattct cttttaatat tccatacatc
    14701 ctccttatca ctataatgaa ggctctttcc gtcagtggtt tttgcgtttt tatcaaatct
    14761 tgaataagga aaaaattttc gttcgttatt atttttgcaa acgtacagac agtgatagtg
    14821 tgatgtgaca aatttttttg atgtgactac tccaaattga tatttccaaa ttatgtgatt
    14881 aattgttatg aatccatttt catctaaagc agttagtata tctttcaaat tattccatcc
    14941 agaaaataca tacatacttc cagatttttt taaaatacga ttaatctgac tcatccaatt
    15001 ataagtaaaa ttgtaataat ccttagattc aatttcatta tatccaggta aaactcgtga
    15061 agcaattcta ttataatttg cttttttagg tttgaagtta atagcaaatg gaggatccgt
    15121 aataactaaa tctattttat tatctggaat atctttcatt ccatcaatac aattttgatt
    15181 atagatttta ttcagactga attttttcaa taattatggt aatcgtatgt tacataataa
    15241 ttaattaatt aaaaaaatac aaggaataga tatcagtttt attatctata tttgatctta
    15301 gattttattc ggttaacttg tgccatttct tctttcagat gtttcttaag tagtctttca
    15361 tgctgagctt tatgcttcca ataacagttc aaaactgctt gtcttgaatt ggctttcttt
    15421 aatcctgcct gaaactgtct atgaattgag gctacttctg ttgtgtaact tgccatatac
    15481 ttatttcatt taattataga taatagtttc ggatacgata atcatgctta catattcatg
    15541 ttaatgctat caataggggt atcctactat acaaggggat cataaaacta ttacatgact
    15601 caatgaaaaa atcttcatgg aaaagattgc tgttgtcact ggcacttcca ctggaatagg
    15661 gtttgagacc gctttagcac tagcaagaga ggggtattat acatacgcca cgatgagaga
    15721 catcacaaaa ggtcaaaaaa ttaaagagtt gggacaaaaa gagaatttga aaattaatgt
    15781 gttaaaactg gatgtagatg atgagaattc ggttaaaaca gctatacaaa aaattcttga
    15841 tgaaaaacat agaattgacg ttcttgtaaa taatgcaggg tgggctctct ggggatgtgt
    15901 ggaagatgta tctgttgatg aatttaaggc acagtttgat acaaacttct tttcaataat
    15961 aagactaatt cagaaagttg gaccgacaat gagaaagcaa ggttctggaa caatagttaa
    16021 tgttagttca gttgtaggtc gtattggatt tccagcatcg cctgcataca ttagctccaa
    16081 atttgcttta gaaggattaa gtgaatcctt gagatttgaa ctagcaccat ttggtgttaa
    16141 tgttgtgata attgaacctg gagtcatcaa aacaaacttt atgaaaaaca tgaagatggc
    16201 aaaaaaatat gggccagagt ctgtttacac ggacattact gacaaggtgg tgtctggcat
    16261 taagatgatg tgtgagatgg gaaccgatcc aaaggaagtt gcagatgcta tagtcaaggc
    16321 tacaaaagat gagaacccat taccaagata cattgttggt aatgatgcat cgatgttctt
    16381 agaggcaaag aagaacaaaa cagatcttga gtttgaaaat tacataaaaa aagaacttta
    16441 tggagaatag atattcagtg ataactctct ttaacctatg ttttattcaa tttctgtggt
    16501 tagattgata tacagttttg ttcaattttt ttctaagtcg tattagataa gtgaaatata
    16561 tgaaatggaa aacattagag cataaaggaa tattatttcc tcctgagtat gaatcagtag
    16621 gtattaagat caaaatcaat ggagaaaatg tatctcttac attagagcag gaagaaatgg
    16681 tttatcaatg ggcaaaaaag aaggatgccc caaaaccagg agctacagaa aaatatgtcg
    16741 aggatcctgt gtttcagaaa aattttatca aagattttgc taaaactttc aacaataaat
    16801 tcaaggatct aaaatatgcg gatattgatt tcacacaatc atacaaactt gttgataaag
    16861 aaaaggaagt caaagaatta atgacaaaag aagaaaaaaa ggcacttgca gtagaacgga
    16921 aaaaaatccg tgaaaatatg aagattagtt atggaaaagc aataatggat gggaaagaag
    16981 ttgatattgc aaactacatg gcggaaccac caggaatttt tatggggaga ggtgagcatc
    17041 ctatgcgcgg acgattcaag ccacgagtga cagcaaaaaa tgtcacgctg aatcttggta
    17101 aagatgcaaa aattccagat ggtaaatggg gaaaaatcgt acatgataaa gattcaatgt
    17161 ggattgcaag ctggatggat atactaactc aaaaacgaaa atatgtctgg ttagcagaca
    17221 cagctggaat taaacaagaa cgagatcaag ctaaatatga taaagcaaaa aaattagcca
    17281 aagagatcga aagtatcaag acacaaatca gtaaggatat gcaaaataag gatccaagaa
    17341 ctcggaaaat atctactgct tgttatctga tttatcgtac tgcaatgaga gttggagatg
    17401 aaaaagatcc tgatgaagct gacactgtgg gtgccacaac attacgaaaa gaacacatca
    17461 aactttctgg aaatgcaatc gagtttgatt tccttggaaa ggacagtgtg agatggaaag
    17521 aaaccattcc agcagagggt caagacaaac agtttcatga taatctgaag gagcttgttt
    17581 caaacaaaaa aaataaccaa gaaatttttg atgatatcac atcaagacat gtaaatgtat
    17641 attattcaac aatcgtgaaa ggtctcacgg caaaggtctt tagaacatat ctggcatcaa
    17701 gtgtagtttc aaaatatctt cgtgcccatg ataacatcaa atctgaatca aatatgaaaa
    17761 aactattcca cgcaaaatta gcaaacctag atgcggctaa aatgtgtaat cataaacgta
    17821 ccataccaaa gaattttgat caaacattag aaaagaaaaa aaatactcta aatgacgtag
    17881 aaaaagcaaa accatggcaa aaatatgaag aatcgttgaa aaaaacacaa tctaagatta
    17941 taaaaacaga agtgcaaaga gaaacccaga agaaacagat cagaaaaatt aaaactatga
    18001 taaaaaagaa aaaagaaaaa cacactgaac gtattgaaaa actacaactt caaatagatc
    18061 tgacacaaaa aacacgagat tataatttag gtacatcact gagaaattac attgatccaa
    18121 gaatctttca aagttggact gatgaagttg ttgctgaatg ggaaaaactt tacacatcag
    18181 cacttcagaa aaaatttcta tgggtaaaaa atgtagattc taaatggtct gaaatttcaa
    18241 aatattatta gattcagaac gatttaattg tactagaaat tcatgcgtat tatgccgggg
    18301 tagctcagcc tggcctagag tgccagcctc tccaaagaag ggcaatactc ataatctgga
    18361 ggtcgtgggt tcgaaaccca cccccggcac tcacaactat atcaataaca aaggctgatt
    18421 caatggttag atagtattaa atttagtttt ttgacgatcg aattaacatg tcagaattgg
    18481 atgaatttgc tgaagcattg atgggacaaa tttcagtaga acttgacgaa gagaaagaaa
    18541 tagcaacatt atcaaaaaaa attaatgatg ataaagattt tacaataaag tttgatgatg
    18601 ttaaaagtat agtacaaaaa atatttccag atttgaagca aaaagtttat gattacactg
    18661 gattagaaat ttctaatgag ttatctatag aatatttagg attagatgga ttcaagaagt
    18721 taaagggtaa aaaagtattc actgataatg cacgagaatt tattgataaa ttatttgatg
    18781 ccgttacaaa aaatgattta aaaaaaattg cagagattat tggagaagat actgctaaat
    18841 tcttggttta ctctacttat gtaaaatcat acatttcaaa attaacaact acatatggtg
    18901 attatctcga ttctaagatt taccttaaca tgtttatttt aggagattat ccaaaaataa
    18961 ttctgtacaa acagggacct ccatatcaga tgaaatctga atctgtaaaa tctggatatc
    19021 ttggggctct aaaaatgact gttctagaag aaataatcca ttcagtacaa accaatctac
    19081 aaaaattaaa catacaagct gttgtacagg ttaatacgat aaatgaggaa ttggcaaaaa
    19141 ctatcctggg attagatgag aaaacagtaa ctgaattgac cgagtattta caattacaat
    19201 tagtaccaga ggaattcaag attgcaaaaa aagccaattt attttttatg ttaaatcctg
    19261 ataatttcat cacaaatgtt atgggacctg atgtgatgac atacacaaga gtggaaattg
    19321 acccaaagat ttcagatttt ataccttcgt tagaagcgat ttatcaaaga tggttgaaac
    19381 ctattcaatc acaacatgca atatttacta ctatggaagg tatggcagaa tttctagtac
    19441 aacgaatatt aaaagatgat acagattttc aaaattatct tactacgttt gtaggtactg
    19501 attattcagc atatagtgta aaaaagagta caggtaaaga attcactgaa tatctattca
    19561 atgagtttgg taaaaataca tttgaaaaat taattataga tccgcccaat acaaaagaat
    19621 tgaaaaatcc acaactatat ctaaatagag ttggatagca ctttgtttat tgacgacaga
    19681 aaagtttgat ccatttgtca cagaatggct gtctttttca caggatccaa aatataattt
    19741 aatagaaaaa tgtctcaaac ttgcacaaat cttggattat cctgaactaa atatcacaga
    19801 ctatattgaa aaaataaatc aaattggtaa ctctctaaaa ttaaaaatag atgatgttga
    19861 aaatccaata tatctcattt caatgctaag tgaacatatt tttgaaaatt atggtttttc
    19921 tggagatgat caaaattatt acgatcctag aaataatttt ctaaatactg taattgataa
    19981 aaaatccggt atccccatca cgctttcaat tgtatatact gaaatagcaa aatacattgg
    20041 acttgatctg aagattgtgg ggtttccagg tcatgttatt gtaaaatata atgatgatat
    20101 tattttagat ccattttata gaggtaggct tcttacaatt gaaaatttag aagaaattct
    20161 acacagtaat tttggtgaag atgttgagat gatttctgaa tatcttgatg aggcaacaga
    20221 agaacaagtg ttaactagac tactaagaaa tttgaaaaat gcatatacac aatcatttgc
    20281 atatgataaa gctatgaaat gtgcaaatat gattttgggg atacaaccag attctccaga
    20341 agaaattcgt gataaaggaa tattagaaga acgactttta cattatgata aggcattacc
    20401 attactgaat aaatatctcg agatgcaacc agaagcagaa gatgtagatt ttattcttga
    20461 attaattaaa aatgtaagag aaaaatctaa tcaatgattg atgatacatc gttacccttt
    20521 ctcaccattt taatctcatg acgtgataat ttattcttga atgcattttt tagaaaatct
    20581 atttcgttac gtaatgaagc tacttcatct tctaattttg caactctttc gtaaatcgtc
    20641 tcagattcat cagtttgttt aggcatgtga tcaaccaaat aaaagacctt aaaaagttat
    20701 tggtaatatc tgctaataat attaaagctc gaactcttgg ttgcaagact ggcatttacc
    20761 tctttccttg cccacttggc caattataaa aattctacct attgtttgac attcaggaca
    20821 cataccagtt gtaacatttt ttggaccgct ttttacaatt ttttgagttt tccgtcttcc
    20881 cattggaagt tttgaattaa tcgtactttt aagtttaatt cagcgcgggg agagggattt
    20941 gaacccacga gttcttgcga acataggatt agcaatccta cgccctacca agctaggcga
    21001 tccccgcaca gtgatattaa attaatttgg ttaattagta taacgtgaat ttaagatagt
    21061 aaaaatcatt aatcgtttca ttaaactaaa tatttcaaaa tgaatttagt tttctgaagt
    21121 ttgagtatag tcttggacaa tatttctcaa tggtgttcgc ttgccattaa caatttttag
    21181 atcaacacga aaagttccat tatcaacatt gactatatta tacgagtttt caaagaatcc
    21241 acgaactctt tctgaagaag cggtgccagc gtttgcaatc gataatgtat taaaatccca
    21301 aatccacggt ctgtgtttgt gcccacataa aactagattc acattagaat ctagaattgt
    21361 acgtaataca tctccagcat caataacggt taatcgatca gaaccagtat ctggaatccc
    21421 aatcaaatga tgatgcattg ctactatctt caatttattt tcatattttt tcaaagttcg
    21481 ttctagccat agattttgat gatgacccac ttcaccttca tcacgatctg gtctagcaga
    21541 accaagtgtc acaagtatta catcattccc aatgggattc tctgctttaa atggaaaata
    21601 ttttttgaat aataaatatc ccgtatttct ataatcatga tttccactaa ttgcgattat
    21661 tttctctgtt ttaatctgag aaactaagtt tttacatttc tcatattgtt caattaaccc
    21721 ttcatttgtt agatcgccag tgatcacaac tgcctctggt tttaatgaat ttatttcatc
    21781 aatgacattt ccaaatactt cttctcgaaa ttgagctcca atatgtagat ctgataattg
    21841 aactatctcc atctgtgtag attaactaca cttcatattt taaacgattg attgatgtaa
    21901 atttaacaaa ttgagaaatt tcaagttagg gttaaataat taaaatttag aatatgtaat
    21961 atgacaaaaa aagcagtggt gatgaaagga gatgggatag gacctgaagt agtggattct
    22021 atgctcaaag tgctcaaaga atgtaattta caatcagaac taatccattg tgaggtagga
    22081 tcagagcaat gggaaaggaa tgggttcaaa gaaaaatcat acattccgga taaaactatg
    22141 gaggcacttg atgatgctga tgcttgtttt aaaggaccaa ccacaactat accaaaacca
    22201 gatgcaccaa aaagtgtagc agttacactt cgtcaaaaat ttgagttgta ttctaatata
    22261 agaccgatta aaacattcaa acgattaacc ccaaatagaa acttggactt tgtttgtttc
    22321 agagaagcca ctgagggatt gtacagtgga attgaagtgc aaatatcaga tgatgcagct
    22381 atagctatac gtcagattac aagaaaaaat tgtaggcgat ttattttatc tggatttcaa
    22441 tgggctaaaa aatacaatct taaaaaaatg actggtatta caaaacgaaa tatactcaag
    22501 aaaactgacg ggattttttg ggaagaatta gaaaactgcc agaaagaatt tccaaatatg
    22561 gaattggaag agatctatat tgataatatg agtcagcaac tagtaataaa tcctgaacaa
    22621 tttaacaact cagttttatt aagtacaaat cttttcatgg atataatttc tgaactagca
    22681 tcagggattg ttggttcaat cggattaatt tattctgcaa atatgggtga ttcctttgca
    22741 atgtttgaag cggcacatgg aagtgctcct tccatcgccg ggcagaataa ggctaatcca
    22801 actgcaacta tacttgcagg tgcttggatg gctgaatatc ttggtgaatc agatatcaaa
    22861 aatgcaatat tttctgctac tgaacaagtg atcaatgaaa ataaagcaac cactagtgat
    22921 attggtggta gtgcaggtac aacacaaatg acagatgcaa tagttgtata tgctaaacaa
    22981 aaactaagaa ggtaatttag ttgcctcaac caatattaat tcttcaaaaa ataatttctt
    23041 ttttgcagca atctgagctc tgaaaccttt caactcagta taatcaatca gtttttgaaa
    23101 attagacagt gatgatgtaa catagattaa ttttccgcct ggtattacac atgattgaac
    23161 tgaatcgata attttttttg gtatttctaa cccatctttc ccaccatctg tgcgaatatc
    23221 caatatttca ttggtatcaa ggtagggtag attacaaatc acaaactcaa acttgtaatt
    23281 caaagcgtca gctccattac agcatatgat attgttcgaa aacatatttt gtttttttag
    23341 cgtaaaaaaa ctcaaatccg ttgccactac gagaggaaaa gatctcgata aaagatctgc
    23401 taaatatcca gaccctgtac ctatatccaa taccgattta ccgctgattt tttctatata
    23461 atccgccagg aagaatgtat cctctgctgg aggatacggt tcaatttcta ataatttttt
    23521 ttgcaatttt tattatttca tctccagata agtcatctaa tctttcctta gatgttgaat
    23581 taatcccgaa ttgttttaaa acattctgaa gcgttttacg cctatatgaa aacattcgat
    23641 tgactgttga aattattgtc tttgatacta tcttcttttg tgtcaaaaaa attacaacgg
    23701 aatctacttt tggtactgga taaaaatttg atttttttac atccattata atttttaatt
    23761 gaaagccata atttgttaga attgatattg cagtatgatt tttactagaa agtaacttat
    23821 ctgcaaattc tttttgtacc ataatcacag cacgagagaa attcttctga agtaaccatt
    23881 ctattgattt tctactcttt gaataaggaa gatttgaaat aaaaatagta aaactatgat
    23941 ttgatttgaa cccatcacca tactctagag ttaaattttt ttcatcatgg aattttgatt
    24001 ttgctaaatt gtacagattt ttatcaattt ctatggatat tattttattt gcttttttgc
    24061 acatgtatgg aagtaaaatt ccatgaccgg tgcctatttc aaatacaaca tcattcttgg
    24121 tgatttttgc ttcagaaact atgtgatttg cgattgtgtt tgattttaaa aaatgctgtc
    24181 cgaagttttg acgttttttc atcgtttaac aaagagattc attcttgctt ttccagaaat
    24241 ctcttcatgt attctttttg atatattagt aatcaaatcc ttcaagccaa cacgttcttc
    24301 aaaatcatga tagctctcga atagcttttt ttctcgttcg tcaagaataa gattgagata
    24361 tgtcttacca atacctggaa tcaactctaa tgcatgtatt cttggtgtga gaggttgtgc
    24421 cttattcaag tagtctacaa accttttctc attttcttta attattaatt ctactatccc
    24481 tgaaatttca ctttgtgctg taacagaaat tttggcataa tctaatttac ctaaaacaga
    24541 ttgaactttt gttcttcctt cttttccaat gtatattttt tctcctattt cgaaagttgt
    24601 gtcctcaagt ccaagtattt ctaaaagtgt taacctctca tcacctattg caattataat
    24661 atttcctgtt ctgcctttaa cagtagtcga ttttccacgt ggtttaaaat ccaacacatg
    24721 tgcatattcc tcatatctcc ttggttgggt attccccatg tccaactatg tcacctaaat
    24781 tatgaatgct ccttaattat tttgagtgtt ttttccagag tttctgaaag aattaatttt
    24841 ttccacccaa atgtaaaaga acgtaattct ggtaaagact ctggcataat attcaaaatt
    24901 tctacagcct cctcttcagt taaaccacat tctttgatta gatcttgctt cattttcttt
    24961 gctatttttg gatcaacatt actaaatttt gaaacataat cataagtcca acgttgaatt
    25021 tgatctaatg attcagcgtc gacctttcct aagatttctt tgacttctgc caatgaaatt
    25081 aattgtttct tttttacttc ttccataatt atacaccgaa tggtttaatg tgatcaaatc
    25141 tagtgattaa ggtttttgat ttattaccaa gtttaatatc aagaacaacg ctacgtctac
    25201 caactttact cacagtccca acttttccgt gatatcttct gtgtggaagt cccttatgct
    25261 gtcgtggatc aataatcacc aacgctttat ctccatcatg atattcatga agtaagaatg
    25321 aaacgcctct aggagcatct tttgtcataa cagatctaga tttatgttta aatccatgag
    25381 aacggccacc tggcttttta gtagtcatat cgaatatgag tcaaaatccc ttattttaat
    25441 actgacgctc atccaaacag cttcattagt ttctcaaaga aatcaaggaa cgaccattcc
    25501 tttggcacgc acagattatc cttcagtatg gttccatcac cacaggttac tttgtccata
    25561 tcaggctctt gatccatgat tatctctgga atgacttctt gctcatattc aatctcaaac
    25621 tttgactttt cttctaatgt taacgcctgt ggaagatttt cgtttacctt cagcgcactg
    25681 gtcagataca atatctccgg aattccaaac ttgttctctg tctcaaccac aatgtgtgat
    25741 gcgtccattg gctttgcaaa tatcatagta aagaccacct caaacatttc cagattcttt
    25801 acgtcagggt cttgggttat cctgtgtgta gcagctgcaa cttgaaacat cttagccttt
    25861 gatacgctgt atttgtcact catgattgaa tagtcgatat gtgcaccatc ttcatagttt
    25921 gttggcgaat tgaagattcc agtgtacact gatgcgaagt gaatttttgt atgtgtgggg
    25981 ttgttaagtg tactaagtgt gactgttacg ggctgtccaa ccagaacctc ggtggtcgga
    26041 acgttagtcc aggtgtctat aattcgatat tcatcattgt tgattgatat ggttccacag
    26101 tttccaagga tgatatgttt tgaacaggta ccccttcatt ctttttaatc ggaccagaaa
    26161 atgaaatagt gaagattcat ggagcacaac catatagtac ttttaaacaa acttttgatt
    26221 cacaattaga aaaataaaat ttctgataac ctgatcaaac tt
//
LOCUS       DASCPJ010000079         4208 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001240601, whole genome shotgun sequence.
ACCESSION   DASCPJ010000079 DASCPJ010000000
VERSION     DASCPJ010000079.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001240601"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="QGF01_03675"
     CDS             complement(<1..209)
                     /locus_tag="QGF01_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HJN20025.1"
                     /translation="MEQDISNIDFSKGDGIVPVIVQDVNTKEILTLAYTNKESLGLAI
                     KTGNSWFWSRSRNKLWMKGEESGNT"
     gene            complement(244..1047)
                     /gene="hisF"
                     /locus_tag="QGF01_03680"
     CDS             complement(244..1047)
                     /gene="hisF"
                     /locus_tag="QGF01_03680"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HJN20026.1"
                     /translation="MTLTKRVIPCLDVAKGRVVKGLNFKSIKDAGDPVLLAEKYSDEG
                     ADELVFLDITASEEKREIIKSLVSKVAQVINIPFTVGGGVKTLQHARDILLSGADKVA
                     INTGAVKNPQIISELMELFGRQCIVVAIDVKRNYNIKNQKNIFSINDKKFWFEVFIYG
                     GKKETGLDAIEWAKKVESLGAGEILLTSIDMDGTKDGYDIELTKKIVNSVSIPVVASG
                     GCGKPDDMIDVFQKTNVEAALAASIFHYETHSVNRVKKIIKENGIPVRV"
     gene            complement(1044..1751)
                     /gene="hisA"
                     /locus_tag="QGF01_03685"
     CDS             complement(1044..1751)
                     /gene="hisA"
                     /locus_tag="QGF01_03685"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HJN20027.1"
                     /translation="MKIIPAIDVMDGKVVRLYRGDPSKKTTYSDNPLEIASKWQSAGA
                     DMLHLVDLDATLGLGSNFELIQKMAKAVSIPVQVAGGLRNENIIQNALDFSERVVIGT
                     LAFKDKVILDKLRTIYGSKRIVISVDHKDGLIVVNGWQESTKLGLIDAVNEFVAMGFS
                     EFLSTNVTRDGTLEGPDLSLIQQINKTEGTNVIVSGGISNIGDVAKVKKTNSFGVILG
                     KALYENKISIEEAKKIA"
     gene            complement(1748..2353)
                     /gene="hisH"
                     /locus_tag="QGF01_03690"
     CDS             complement(1748..2353)
                     /gene="hisH"
                     /locus_tag="QGF01_03690"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HJN20028.1"
                     /translation="MKVAIFDYGAGNIFSLKNSLEKNGAEVDVITGFDNLQKYSGLLL
                     PGVGNFDPAIRSIRDYSAIDFNEYVSQDMPVLGICLGMEMFFEKSEEGKEKGLNVIDG
                     EVVLLPTSMKIPHMGWNNLEIKQSSKFLEGVDDGSWVYFVHSYRVKPKASELIVAETD
                     YGIKVPAIVETKNFIGTQFHPEKSGEVGSLMLKNFLRECQK"
     gene            complement(2356..2943)
                     /gene="hisB"
                     /locus_tag="QGF01_03695"
     CDS             complement(2356..2943)
                     /gene="hisB"
                     /locus_tag="QGF01_03695"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HJN20029.1"
                     /translation="MKPRTSKINRETKETGVKATVNLDGTGKTSIQTGISFLDHLITS
                     FAKHSMIDLSIKGKSLDNIEHHLIEDVGITIGAAIDKALGDRSGITRFSFASVPMDES
                     LAETSIDLVKRPFTKITLLVKRTKIEGISKEDIEHFFSSLTQNLNSCIHITVKYGEND
                     HHKIEAAIKSFAVAFRLAASPDKKRKGVPSTKGAM"
     gene            complement(3005..>4208)
                     /locus_tag="QGF01_03700"
     CDS             complement(3005..>4208)
                     /locus_tag="QGF01_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20030.1"
                     /translation="NYAQLTKTLADAVIDHEINHETELLYTFVTLDGEPKDRFMDMSY
                     GGSVGLESLLLAYEVTSDRTYLDQVKRTILAYWELRDKNTNLIPSKINADTKSVKDSF
                     MEQYGAGIFLKLLLHYYYLTEDPEIYKIIEDYTDAVVNYYWDGKTWNYRVDYDGTVWS
                     DVIEGNYGKLDDALFLVYDLDPSRFQKAYELAKSDYDFSFKDKNVMANGLVIHSVKDD
                     GSPESPESMMTYAFIINQNPAVRLYHDTTNPQYIQDMKDFYEKVILHHKRQYGYIYGV
                     DAYTLGKTELGTLLNQYAPGTIANKINLSFVPSNSVNIVWTKIGNFEITEPFIVNFNE
                     PGRFNAISFDYAEKSIFFEAVENEGTITFSGEIKSVLVDGGNYPNFSGKTLNTLEGKY
                     NYKVTLVD"
BASE COUNT         1188 a          847 c          614 g         1559 t
ORIGIN      
        1 tgcgtgtttc cagattcttc acctttcatc caaagtttgt tacgcgatct actccagaac
       61 cacgaattgc ctgtttttat agcaagccca agtgattctt tgtttgtata tgctaatgtc
      121 agaatctctt ttgtatttac atcctggact atgacgggaa ctataccatc tccctttgaa
      181 aaatcaatgt tgcttatgtc ttgttccata attcatcata aactgtatgt ttgttaatct
      241 ttactatact ctcactggaa ttccgttttc cttgatgatt ttttttacac gattaaccga
      301 atgtgtttca taatgaaata ttgaagctgc cagagctgcc tcaacattag ttttttgaaa
      361 gacatcaatc atatcatcgg gttttccaca tccacctgat gcaacaacag gtatagaaac
      421 tgaatttaca attttttttg tcagttctat atcatatcca tctttggttc catccatatc
      481 aatgctagta agaagtatct ctcctgctcc caatgattca actttttttg cccattcaat
      541 tgcatctaaa ccggtttctt tttttccacc gtaaataaaa acttcaaacc agaatttttt
      601 atcatttatt gaaaatatgt ttttttgatt tttaatgtta tagtttcttt ttacatcaat
      661 tgcaacaaca atgcactgtc tgccaaaaag ttccatcaat tcagaaatta tctgtgggtt
      721 ttttacagca cctgtattta ttgcaacctt atctgcacca ctgagcaata tgtctctagc
      781 atgctgaagt gttttaacac caccaccaac agtgaatgga atgttaatca cttgagcaac
      841 ttttgaaact agtgatttga ttatctctct tttttcttca gatgctgtga tgtcaagaaa
      901 tacaagttcg tcagctccct catcgctata tttttcagct agtaaaacag gatcaccggc
      961 atccttgatt gatttaaaat ttaatccctt tactactctt ccttttgcaa catcaagaca
     1021 tggtattact cttttagtta aagtcatgct attttttttg cctcttcaat tgaaatttta
     1081 ttttcataca gtgcttttcc taatatgaca ccaaatgaat ttgtcttttt gactttagca
     1141 acatcaccaa tgttggaaat tcctccacta acgattacat ttgtgccttc agttttgtta
     1201 atctgttgta ttaagctgag atcaggacct tctaatgttc catctctggt tacatttgtt
     1261 gataaaaatt ctgaaaagcc cattgctaca aactcgttta ctgcgtcaat taatcctaac
     1321 tttgttgatt cttgccatcc atttacaact atcaaaccat ctttgtgatc aactgatatg
     1381 actattcttt tgcttccgta tattgttcga agtttatcca gaattacttt atccttgaat
     1441 gccaatgttc ctatgacaac tcgttctgaa aagtccaagg cgttttgtat aatgttctca
     1501 tttcgtaatc cccctgcaac ttgtacagga atgcttactg cttttgccat tttttgtatt
     1561 aattcaaagt ttgaacctaa tccaagtgtt gcatcaagat ctaccaagtg gagcatatca
     1621 gctcctgcag attgccattt acttgcaatt tctagtggat tatcactgta ggtagtcttt
     1681 ttgctggggt cccccctata gagcctgaca acttttccat ccataacatc aattgctgga
     1741 ataattttca tttttgacat tctcttagaa agttctttag catcaaggaa ccaacctcac
     1801 ctgatttttc agggtgaaat tgtgttccga tgaaattttt tgtttcaact attgctggta
     1861 ccttgatccc ataatcagtt tctgcaacaa taagttcaga ggcttttggt tttactctgt
     1921 acgagtgcac aaagtaaacc catgaaccat catcaactcc ttctaaaaat ttacttgatt
     1981 gtttaatttc tagattattc caacccatgt gcggaatttt cattgaagtt ggtaagagaa
     2041 ctacctcacc atcaattaca ttcagacctt tttcttttcc ttcctcactt ttttcaaaaa
     2101 acatctccat cccaagacat attcctagta ctggcatatc ttgactaaca tattcattga
     2161 aatcaattgc agaataatca cgaatacttc taatcgctgg atcgaaattt cctacacccg
     2221 gcagtaataa gcccgaatac ttttgcaaat tgtcaaagcc tgtaatgacg tccacttcgg
     2281 caccattttt ttctagcgag ttttttaagc taaaaatatt tcctgcgccg taatcaaata
     2341 ttgcaacttt caatactaca tcgcaccttt ggtgcttgga actccctttc gttttttgtc
     2401 gggtgaagct gctaatcgaa atgcaactgc aaatgatttt attgcagcct caatcttgtg
     2461 atgatcattt tcaccatact ttacagtgat gtgtatacat gagtttagat tctgtgtaag
     2521 ggatgaaaaa aagtgttcta tgtcctcttt tgaaattcct tctatttttg ttcgcttaac
     2581 taacagtgta attttcgtaa atggtctttt gaccaaatcg attgatgttt ctgccagtga
     2641 ttcatccatt ggaactgatg cgaagctaaa cctggtaatt ccagaacggt ctcctaatgc
     2701 tttgtctatt gctgctccaa ttgttattcc tacatcctca ataagatggt gttctatgtt
     2761 atccaaagat tttcccttta tagataggtc aatcattgaa tgcttggcaa atgaagttat
     2821 caagtgatct aggaagctga taccagtctg gattgaagtc ttgcctgttc catctagatt
     2881 aactgtagct ttaacgccag tttccttggt ttctctgttt atttttgatg ttcttggttt
     2941 catatgtatc ccgaatttct ctttactctt aaacctgatt taatactaac tatctgattc
     3001 attattaatc caccagtgta actttgtagt tatatttacc ttctaaagta tttagtgtct
     3061 ttccactaaa attgggataa tttccgccat caactaaaac actttttatc tcacctgaaa
     3121 atgtaatagt cccctcattt tcaactgctt caaaaaatat ggatttttct gcataatcaa
     3181 agctaatagc attaaatcta ccaggttcgt taaaatttac tataaatggt tctgtgattt
     3241 caaaatttcc aattttggtc catacaatat ttacactatt tgatggtaca aatgacaagt
     3301 ttattttatt tgcaatcgtt ccaggcgcat attggtttaa caatgttcct aattcggttt
     3361 ttcccaacgt gtaagcatcc actccataga tatatccata ttgtcgttta tgatgaagaa
     3421 tgactttctc gtaaaaatcc ttcatgtctt gaatatattg tgggtttgtg gtatcatgat
     3481 aaagcctaac tgcaggattc tgattaatta tgaatgcata ggtcatcata gactccgggc
     3541 tttcaggaga accatcatct tttacactat gaataacaag tccatttgcc ataacatttt
     3601 tgtctttaaa tgaaaaatca tagtcagatt ttgctagttc gtatgccttt tggaatctgg
     3661 atggatctaa atcataaacc aaaaacaatg catcgtctag tttgccataa tttccctcaa
     3721 taacatctga ccagactgtt ccatcataat ctacacgata attccaggtt ttaccatccc
     3781 agtaataatt cacaacagca tctgtataat cttcgataat cttgtaaatt tcaggatctt
     3841 cagttagata ataataatga agtaaaagtt tcaaaaatat tcctgctcca tattgttcca
     3901 taaaagaatc tttgacactt ttagtatctg cattaatttt tgatggaatt agatttgtgt
     3961 ttttatctct taactcccaa tatgcaagaa tagttctttt gacctgatct aggtatgttc
     4021 tatcagaagt cacttcatat gcaagcaaaa gagattctaa accaaccgaa ccaccgtatg
     4081 acatgtccat gaaacggtct tttggctctc cgtcaagtgt aacgaatgta tacaataact
     4141 ctgtttcatg gtttatttca tgatcaatga ctgcgtctgc taatgttttt gttaattgtg
     4201 cgtaattc
//
LOCUS       DASCPJ010000080         2048 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001244907, whole genome shotgun sequence.
ACCESSION   DASCPJ010000080 DASCPJ010000000
VERSION     DASCPJ010000080.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001244907"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="bioB"
                     /locus_tag="QGF01_03705"
     CDS             <1..726
                     /gene="bioB"
                     /locus_tag="QGF01_03705"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HJN20031.1"
                     /translation="IDKYELMSPEEVVREAMKAKKSGADSYCLVAAWKEPSRDDFTKV
                     CNIIKEVNDKVGISIECSLGFLTTEQAIILKQLGVIRYNHNLETSKSKFPDICTTHTY
                     QDRVNTLNIAKNAGLELCTGGIIGIGETKKQREELIFEIASFQPEEVTINLLVPIPGT
                     PLEIQKPVKIEEIIRVFATLRFLLPKSIIKISGGREVNLVDDGQKLLLSGANGIISSG
                     YLTMNGNSPEKDIKMLKKINLEV"
     gene            716..1843
                     /locus_tag="QGF01_03710"
     CDS             716..1843
                     /locus_tag="QGF01_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HJN20032.1"
                     /translation="MKSRLSFINQQLEYIKRNNLYRKMYDVKNIGPNISVNSKKLINL
                     CSNDYLGIKSPKISRKQNQSSSRLIAGNDESFKILESKLAKHKSQDSALIFPTGYMAN
                     LGVISSLIQKNDHVYSDEFNHASLIEACKMTGAKITIFKHNDMYDLNSKIKTDSKRKF
                     IITEGIFSMDGDCADLKIISEISQKHDAITILDDAHGDFIIGKDGGGSANYFGVEKKI
                     DIYTSSLSKGLGSFGGYIASQKNVIELSINKSKSFIYTSALPSLFIDDAIKRFDSDRE
                     KRRKKLWENTNYFSTCLKKIGYENKSKSHIIPIMIGKEKLAIEFAIYLRKNGIFVHPI
                     RYPTVPKNQSRIRISITASLTKKQIEFSLDVFDKARKKFQI"
BASE COUNT          830 a          276 c          322 g          620 t
ORIGIN      
        1 attgacaagt atgaattaat gtcaccagaa gaagtagtaa gagaggctat gaaagcaaaa
       61 aaatcaggag ccgattcata ttgcttagtt gctgcgtgga aagaaccctc aagggatgat
      121 tttactaaag tttgtaatat aataaaggaa gttaatgata aggttggtat ctcaattgaa
      181 tgtagtttag gatttttaac aactgaacag gcaataatat taaaacaact tggtgtgatt
      241 agatataatc ataatttaga aacatccaaa tctaaatttc cagatatatg tactactcat
      301 acataccaag atagagttaa cacactaaat attgcaaaga atgctggatt agaactatgt
      361 actggaggaa taattggaat tggtgaaaca aaaaaacaac gtgaagaatt aatttttgaa
      421 attgcttcat ttcaacctga agaagtgaca atcaatttat tggtcccaat tcctggtacg
      481 ccattagaaa ttcaaaaacc tgtcaaaatt gaggaaatta tacgtgtttt tgctactctt
      541 agattccttt tacctaaatc tataatcaag atatcaggtg gaagagaagt aaatcttgtg
      601 gatgatggac aaaaactgtt acttagtggg gctaatggaa ttattagttc tggatattta
      661 acaatgaatg gtaattcacc agaaaaagac ataaaaatgc tcaaaaagat taatcttgaa
      721 gtctagatta tccttcataa accagcagtt agaatacatt aaaagaaata atctctatcg
      781 gaaaatgtat gatgttaaaa atataggtcc gaatatcagt gtgaattcta aaaaattaat
      841 taatctttgt tctaatgatt atttaggaat aaaatcacca aaaatatcta gaaaacaaaa
      901 tcaatcaagc tccagattaa ttgctggtaa cgatgaatca tttaaaattc ttgaatctaa
      961 acttgcaaaa cataagtcac aagatagtgc attaattttt ccaacaggtt acatggcaaa
     1021 tcttggagtg atatcttcac ttattcaaaa aaatgatcat gtttatagcg atgagttcaa
     1081 tcatgccagt cttattgaag catgtaaaat gactggggca aaaattacaa tttttaaaca
     1141 taatgatatg tatgatttaa attccaaaat aaagactgat tctaaacgaa aatttatcat
     1201 cacagaggga atttttagta tggatggtga ttgtgctgat ttaaaaataa tttcagaaat
     1261 atctcaaaaa catgatgcaa ttacaatatt ggatgatgcg catggagatt ttataatagg
     1321 aaaagatggt gggggatctg caaattattt tggtgtggaa aaaaaaattg atatttacac
     1381 tagtagtcta agtaagggtc ttggatcatt tggtggatac attgcatcac aaaaaaatgt
     1441 gattgagtta tctataaaca aatcaaagtc atttatctat acatcagcac ttccttcatt
     1501 atttattgat gatgcaatta aaagatttga ttctgacaga gaaaaaagaa gaaagaaatt
     1561 atgggaaaac actaattatt tttcaacttg tctaaagaaa attggttatg aaaataaatc
     1621 aaaatcacac attataccaa taatgattgg aaaagaaaaa ctggcaatag aatttgcaat
     1681 atatcttcgt aaaaatggaa tttttgttca tcctataaga tacccaacgg taccaaaaaa
     1741 tcaatcacgt atccgaattt caattactgc atcattaaca aaaaaacaaa ttgaattctc
     1801 attagatgtg tttgataaag ccagaaaaaa gttccagata tgataattat ctatttagat
     1861 caaatcctcc aaacattgga gaacccatca aaaaggctgc aataactata atggctaaag
     1921 caacccctgc aactatcaat ctactattca tatcaaaatt aaaaattatc cagttaaaaa
     1981 gtcattcaat atcaattatc aaaaacgagc ataaaaaatg aatagaagtt gtatcaccat
     2041 aaaaattt
//
LOCUS       DASCPJ010000081         3017 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001260594, whole genome shotgun sequence.
ACCESSION   DASCPJ010000081 DASCPJ010000000
VERSION     DASCPJ010000081.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001260594"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="QGF01_03715"
     CDS             complement(<1..506)
                     /locus_tag="QGF01_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HJN20033.1"
                     /translation="MSDDSEKNQLDMIGMMLGKHMDGTVDTDTMIIKTQERLVNSLEH
                     GIEYDGTVEESDNFLREHVWKKLPMVVMYVDIVGSTQMVLSLPEEKVAMLFSSFTQEM
                     AILIEQNAGMVLKFVGDAVIGYFIDASNLLASDNAVTCARSMIRVINEGINPVLAKSG
                     YPELAVKIG"
     gene            652..1485
                     /locus_tag="QGF01_03720"
     CDS             652..1485
                     /locus_tag="QGF01_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20034.1"
                     /translation="MRLGLDTEDEFLIKLNEINEQIQDIFLVEAKKMTEKISVDVMLQ
                     DGTAKKQDTCSMEMIKAFYDKIAKKLTNWTLQTTSTTNNEDLRRDFIKMHAIVDDYRI
                     LLHISTQYHVLLYYKPDYQVMKKQKELADFLTMTEKNEKKLAEKSDQVILKKLKDEGY
                     KDLNPENLFEIFFKDDKIMENVTKEIDKQTDGDLNKIYEQKQSLLKELDNFLLEIYQI
                     EPVLIDEARLVTGEEGFVFNLDVERVNDEQSKQFSSDKMSEETKKNITIRVEEILKVI
                     K"
     gene            complement(1499..2326)
                     /locus_tag="QGF01_03725"
     CDS             complement(1499..2326)
                     /locus_tag="QGF01_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20035.1"
                     /translation="MTDTLSSTIVTLKTEYMTNNTSHAHIHEFIPSSSSNILSIDKNH
                     LELLHQFMLSNPIYSSSHESKIDEIPCTIYEGDVTHFWLDSIKHDTSYAPFYPTWMLS
                     AYTLALISKNLGVNNIIDIGSGDGRIAYCGKVVGMESYGIEIDENLVLLQKSISDDTK
                     VNFNMKLDDATKFDYTSLNLSQPAVFLSGLPEQGELLANSVIEKILNISNLKNNAMFV
                     FMGSYSKRKYTKNDSKFGWGNIIDSFDLDIMKTVTLPTYWTSDQPVDTPFIFTKLHD"
     gene            2417..>3017
                     /locus_tag="QGF01_03730"
     CDS             2417..>3017
                     /locus_tag="QGF01_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HJN20036.1"
                     /translation="MAISYDDVVNAQKTQDNVIRKTSLTFSDTFTKITGSTVYLKNEF
                     EQKTGSFKLRGAYYKIKTLSEEEKRNGVVAASAGNHAQGVAFASALEKIKCVIVMPKN
                     ASPAKVAATKAYGATVVLAGKNYDESWIKAQGIAKTTGATIIHAFDDPQIIAAQGVIG
                     LEIMEQLPDVDEVYVPIGGGGLAAGILTAIKDKNPNVKII"
BASE COUNT         1117 a          475 c          479 g          946 t
ORIGIN      
        1 ccaattttta ccgcaagctc tggatagcca gatttagcta aaacgggatt tattccctca
       61 ttgattactc taatcataga tctagcacat gttactgcat tatctgatgc cagaagatta
      121 cttgcatcta taaaatagcc aattactgcg tcaccaacaa attttaacac cataccagca
      181 ttctgttcta ttaaaattgc catttcctgc gtaaatgaac tgaataacat agccaccttt
      241 tcttcaggca aagatagtac catttgtgtg gaaccaacta tgtccacata cataaccacc
      301 attggtaatt ttttccaaac atgttctctc aaaaaattat ctgattcttc gacagtgcca
      361 tcatattcaa taccatgttc taatgaattt actagtcttt cttgtgtttt tataatcatt
      421 gtatccgtat ctactgtacc gtccatatgt ttaccaagca tcattccaat catatctaat
      481 tgattttttt ctgaatcatc actcaaaatt tggctcacct ctaaaacaaa atatgttttt
      541 catataatgt ctcaattcac ttttagatta taataattgt tatgaaactc agttatcgta
      601 atcaatatct tattcaatta agattttatt tagtataatt ctagttagat aatgcgatta
      661 ggcctggata ctgaagatga attcctgata aaattaaacg aaataaatga acaaatacaa
      721 gatatttttc tcgttgaggc taaaaaaatg acggaaaaaa tatcagttga tgttatgtta
      781 caagatggaa ctgcaaaaaa acaggacact tgcagtatgg aaatgataaa agcattttat
      841 gataaaattg ccaaaaaatt aacaaattgg acattgcaaa ccacttctac cacaaataat
      901 gaggatttac gccgagattt tatcaaaatg catgcaatag tagatgatta cagaatctta
      961 ttgcatatat caacgcagta ccacgtttta ctatattata agccagatta tcaggtaatg
     1021 aaaaaacaaa aagaattagc agacttctta acaatgacag aaaaaaatga aaaaaaactt
     1081 gcagagaaaa gtgatcaagt aatactcaaa aaattaaaag atgagggtta taaggatctc
     1141 aaccctgaaa atctttttga aattttcttt aaagatgata aaattatgga aaacgtcacc
     1201 aaagagattg ataaacaaac tgatggtgat ttaaataaaa tttatgagca aaaacaaagt
     1261 cttctgaaag aattggataa ttttttacta gaaatatatc aaatagagcc cgtattgatt
     1321 gatgaagcca gattagttac tggcgaagaa ggatttgttt ttaatttgga tgtagaacga
     1381 gttaatgatg aacaatcaaa acaattctct tctgacaaaa tgtcagaaga aacaaaaaag
     1441 aatattacaa tcagggttga agaaattcta aaagttataa aataaattat aattagtatt
     1501 aatcatgaag ttttgtaaaa ataaaaggtg tgtcaacagg ttgatcagaa gtccaatatg
     1561 ttggtaatgt gacagttttc ataatatcaa gatcaaaaga atctattata tttccccatc
     1621 caaactttga atcatttttt gtgtattttc tctttgaata acttcccata aacacaaaca
     1681 ttgcattatt tttcaaatta gatatgttta gaattttttc aattacactg ttggctaata
     1741 actctccttg ctctggtaaa ccagaaagaa agactgctgg ctgagacaag tttaatgatg
     1801 tataatcaaa ttttgtagca tcgtctaatt tcatattaaa attaactttg gtatcatctg
     1861 atattgattt ttgtaaaaga acaagatttt catctatttc aattccatac gattccattc
     1921 caacaacttt accacaatat gctattctcc catcccctga accaatgtca ataatattat
     1981 taacaccaag attttttgaa attaatgcca aagtgtatgc agaaagcatc catgttggat
     2041 aaaatggcgc ataacttgta tcatgcttga tactatccag ccaaaaatgt gttacatcac
     2101 cttcatagat cgtgcatggt atttcatcaa tttttgattc atgggagctt gaatagatgg
     2161 ggttactgag catgaattga tgaagtaatt ctaaatgatt cttatcaatt gatagaatat
     2221 ttgatgaaga actagggata aactcgtgaa tgtgagcatg actggtgtta ttagtcatat
     2281 attctgtctt taacgtgact atagtacttg agagtgtgtc agtcatctat accatacaga
     2341 ttcagcattg agattataaa attattagaa atatacagtt acaaaaaatc aaatatggaa
     2401 aaaatacagg caattgatgg caatttcata tgatgatgta gtgaatgcac aaaaaacaca
     2461 ggataatgtt atcagaaaaa catctcttac cttttctgac acatttacca aaattacggg
     2521 ctcgacggtc tacctaaaaa atgaattcga gcaaaagact ggctcattca aactacgtgg
     2581 agcatattat aaaatcaaaa ctctgtctga agaagaaaaa aggaatggtg tagttgcagc
     2641 ttctgctgga aatcatgctc aaggagttgc atttgcttca gcattagaaa aaatcaaatg
     2701 tgttatagtt atgccaaaaa atgcttcacc agctaaggtt gccgcaacaa aggcatatgg
     2761 tgcaacagta gttttggcag gaaaaaatta tgatgaatca tggataaagg cacaaggaat
     2821 tgctaaaaca acgggtgcaa caatcattca tgcattcgat gatccacaaa ttatagctgc
     2881 acagggagta ataggattag aaatcatgga acaacttccc gatgttgatg aagtctatgt
     2941 tccaataggt ggtggtggat tggcggcagg tattttaaca gcgattaaag acaaaaatcc
     3001 aaatgtgaaa atcattg
//
LOCUS       DASCPJ010000082         3122 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001274656, whole genome shotgun sequence.
ACCESSION   DASCPJ010000082 DASCPJ010000000
VERSION     DASCPJ010000082.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001274656"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="QGF01_03735"
     CDS             complement(<1..375)
                     /locus_tag="QGF01_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HJN20037.1"
                     /translation="MATEVIATEGEAMYAWLIWILPFIAALIIPAVGKFSKISTGRVA
                     VGFAAMSAISAAILLPMALDGHEIHDQIDWISAIGLKAGVLADPLSIIMANVVGWISF
                     LIMCYSTGYMKGDKDIVRFWFWM"
     gene            complement(379..1929)
                     /locus_tag="QGF01_03740"
     CDS             complement(379..1929)
                     /locus_tag="QGF01_03740"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HJN20038.1"
                     /translation="MEYLLLQAVFLPLLLSPLAYIIGRRLGHTAATWFTFGLLLYCTT
                     LLIAGSMGETYEEHYPWTELFGEFGFLLDGLAAPFAIMIYVLCTILALYSKPYMSHKF
                     HEFFKQQGIKTVYQSDGTVLAGTSLEQFVNKQSGVYYALFLVFSMGMLGTVLATNLIE
                     FYVFFEVMLIPAFLLIGFWGDHPRRRIALMFFFWTHVGAVILLLGFLAIGLSVGSFDF
                     ADINEADISSDILVPAAVAIIIGLGVKLAAFGFHIWLPYAHGSAPTPISALLSPAMIG
                     IGAYALFRLIVEFLPQTYADLSIWITIWGIGTMFYGGAMALMQDDIKRVFAYSSISQM
                     GYILFGIGSISTLGLAGAEMMYISHALGKGLLFMTAGVLIVQVGTRSLSKLGGLGSKL
                     PITAVCAVIGALTIMGVPPTSGFMGEWMLFYGVLETALEEGNDVRSLMFALGLVATVL
                     TMSYLLWMLKRVFFGKLPENFSKVKEANWYMLSPMMVLAGFTIVVGIYPDIFLQKIMP
                     YMQGVLGG"
     gene            complement(1929..2234)
                     /gene="nuoK"
                     /locus_tag="QGF01_03745"
     CDS             complement(1929..2234)
                     /gene="nuoK"
                     /locus_tag="QGF01_03745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HJN20039.1"
                     /translation="MSNELIDFLIVSIALLAIGIYGVSVKRNFIRMLFAIEIIINAAN
                     LNLVAFARFIPNSEGQTLALFSIAVAAAEVAVGLALIIVAYRMYKNIDVADFRSLKG"
     gene            complement(2215..2730)
                     /locus_tag="QGF01_03750"
     CDS             complement(2215..2730)
                     /locus_tag="QGF01_03750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HJN20040.1"
                     /translation="MVDALFLGFAVLTIVSAIAALEMRSLIYGSIALMGTLGGIAGFF
                     ILLDSPFVALFQIAVYVGSIAVLILFTVMLVKRELIFKMKEDRKRRYAGIGLMLIMMV
                     ALGSLLFSSGLPSITTDEPPVDYKEIGKDFLTYYWPALIVMGLVLAGSITGAIALARR
                     VDGVEDEQRTD"
     gene            complement(2723..>3122)
                     /locus_tag="QGF01_03755"
     CDS             complement(2723..>3122)
                     /locus_tag="QGF01_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJN20041.1"
                     /translation="FVGDGYQFDPTSGVGIAGYKGRHILFHDKCTGCNLCSIACEEIA
                     EAIAMPKVPEQWKHNKKAIMPQIDYGKCVFCGLCVDACPFYALYMTNDYELSSFTKEG
                     LIYTPAQLQVKPQVQQDVEIQIDEKGANHG"
BASE COUNT         1061 a          677 c          506 g          878 t
ORIGIN      
        1 catccagaac cagaatctta ctatgtcctt gtctcctttc atgtatccag tactgtaaca
       61 cataatcagg aaggaaatcc atcctacaac gtttgccatt attattgaaa gaggatcagc
      121 taaaactcca gcctttaaac caattgcaga aatccaatca atttgatcat gaatttcatg
      181 tccgtctaat gccattggga gtaatattgc tgcagatata gcactcatgg cagcaaatcc
      241 aacagctact cttccagttg aaatttttga aaactttcca actgcaggaa taattagagc
      301 tgcaataaat ggaagaatcc agatcagcca agcatacatt gcttcacctt cagttgcaat
      361 aacttctgtc gccatttact atcctcctaa aaccccctgc atgtatggca tgattttttg
      421 taaaaatata tcaggataaa ttccaactac gattgtaaat cctgcaagaa ccatcattgg
      481 agagagcata taccaattgg cttcttttac cttggaaaaa ttttcaggaa gttttccaaa
      541 aaagactctc tttaacatcc aaagaagata tgacattgta agaacagtgg ctaccaaccc
      601 aagtgcaaac atcaatgatc ttacatcatt tccttcttcc aatgcagttt ctagaacacc
      661 gtagaacaac atccattcac ccataaatcc actagttggt ggtacaccca taattgtcag
      721 agctccaatc acagcacata ctgcagtgat tggtaattta cttccaagtc caccaagttt
      781 tgaaaggctt cgtgtaccta cttgaacaat caagactcca gctgtcatga agagcaatcc
      841 cttacctagt gcgtgagaaa tatacatcat ttctgctcct gccagaccaa gtgtagagat
      901 agaacctatt ccaaacaaaa tgtagcccat ttggcttata ctggaatatg caaaaactcg
      961 tttgatatca tcttgcatta atgccattgc cccaccgtag aacattgttc ctatacccca
     1021 tatggtgatc caaatagaca gatcggcgta tgtctgtggt aaaaattcaa caattaacct
     1081 aaagagtgca tatgctccaa ttccgatcat tgcaggggaa agtaaagcgc tgatgggtgt
     1141 tggtgcagat ccatgagcat aaggtaacca aatatggaat ccaaatgctg caagttttac
     1201 acctaaccca attatgattg ccactgccgc aggaaccaga atgtcagatg aaatatcagc
     1261 ctcattgata tcagcaaaat caaagcttcc tacactaaga ccaatagcca aaaatcctag
     1321 aagtaagata actgctccaa catgtgtcca gaaaaagaac ataagtgcaa ttctacgtct
     1381 tggatgatca ccccagaaac ctatcaaaag aaatgctggt attaacataa cttcaaagaa
     1441 tacgtaaaat tcaattaggt ttgtagcaag tacagtacca agcattccca ttgaaaaaac
     1501 gaggaataac gcatagtaca ctccagattg cttgttaaca aattgttcta aagatgtacc
     1561 tgccaatact gttccgtcac tttgataaac tgttttaatt ccctgctgtt tgaaaaattc
     1621 atgaaatttg tgtgacatgt aaggcttgga gtataatgca agtattgtac acaaaacata
     1681 gatcattatt gcaaagggag cagctaaacc atcaagcaaa aatccaaatt ccccaaagag
     1741 ctcagtccaa ggataatgtt cttcatatgt ttcacccatt gatccagcaa taaggagagt
     1801 agtgcagtaa agtagtaatc caaatgtaaa ccatgttgca gcagtatgac caagtcttct
     1861 accaattatg tacgctaaag gtgataataa cagtggcaag aaaactgcct gaagtaacaa
     1921 gtactccatt atcctttcaa actcctaaag tctgctacat caatattctt gtacattcta
     1981 tatgctacaa tgataagtgc gagtccgact gcaacttctg cagctgcaac ggcaatagaa
     2041 aataatgcta gtgtttgtcc ttcactgttt ggtatgaatc ttgcaaaagc aacaagatta
     2101 agattagctg catttattat aatctcaatt gcaaaaagca ttcgaatgaa gtttcgcttt
     2161 actgatacac cataaatacc aattgccaga agggcaatag aaacaattag aaaatcaatc
     2221 agttcgttgc tcatcttcca ctccatccac tcttcgtgct aatgcaattg ctcctgtaat
     2281 ggagccagcc aatacaagcc ccataacaat tagtgccggc caataatatg ttaaaaagtc
     2341 tttacctatc tctttgtaat ctactggagg ttcgtctgta gtaattgagg gaagtcctga
     2401 actaaatagt agggagccta aagcaaccat cattattaac atcaaaccaa ttcctgcata
     2461 cctacgtttt cgatcttcct tcattttgaa aattaattct ctttttacta acattacagt
     2521 gaataagatg agtacagcta tcgagcctac atatacagca atttgaaata gtgcaacaaa
     2581 tggggaatct aacagaataa aaaatcctgc aataccgcct aatgtgccca ttaaagctat
     2641 tgaaccatat atcagcgaac gcatctcaag tgccgctata gcggaaacaa ttgtcagtac
     2701 agcaaatcct agaaataaag catcaaccat gattagcacc tttttcgtca atctgaattt
     2761 caacatcctg ttgaacttgt ggttttacct gaagttgtgc aggagtatag attaaaccct
     2821 ctttggtaaa tgaagataat tcgtaatcat ttgtcatata caaagcatag aaaggacatg
     2881 catccacaca taatccacaa aatacgcact tgccataatc tatttgaggc attatggctt
     2941 ttttattatg cttccattgt tctggaactt ttggcatagc aatcgcctca gcgatctctt
     3001 cacatgcaat tgaacaaaga ttacatccag tacatttatc atgaaataaa atatggcgtc
     3061 ccttatagcc tgctatacca acaccgctag taggatcaaa ttgatatcca tctcctacaa
     3121 at
//
LOCUS       DASCPJ010000083         3173 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001313444, whole genome shotgun sequence.
ACCESSION   DASCPJ010000083 DASCPJ010000000
VERSION     DASCPJ010000083.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001313444"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="QGF01_03760"
     CDS             complement(<1..754)
                     /locus_tag="QGF01_03760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJN20042.1"
                     /translation="MLKFQNKTVLVTGSGTGIGQAITKKFVENGASAVILGRRKEPLE
                     ETAKMLEEIIKEKQSDASIKIFDGVDVSDEKAVTGMFDSLKSENVNLDIVVNNAGVSG
                     PVTCFAHAPLDQFRSTVDIHLTGTFWTSVQALKVMKEGAKIITISTFFAEERPLEQRP
                     YRFRDPYTASQGAKNRLVEAMSWELTEKGIISIGTNPGPVHSDRIYKTVYPKAASEFL
                     RVSGFEDLSPSEVEAANNEIVGLLGESDETIKA"
     gene            852..1088
                     /locus_tag="QGF01_03765"
     CDS             852..1088
                     /locus_tag="QGF01_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20043.1"
                     /translation="MEDSEPETIVLKNWPEKFSDILNEIGVSAVPNEIGKGDVEKGDY
                     YSRCFTQTTRMITNKGSLKITGTDIDAVHIIQKG"
     gene            1091..1468
                     /locus_tag="QGF01_03770"
     CDS             1091..1468
                     /locus_tag="QGF01_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20044.1"
                     /translation="MQDPNPLPWGAQDRFQAHFIVQKDIGEELLNLTARTRLQTTGHF
                     GSKKITGVTWEGGQLADELNTDSSLNKMIAKQSLNDAIIFVDPTDNGVRIYGKWKNSY
                     NFGITKELFDIYNKIAGYIKKLN"
     gene            complement(1461..2000)
                     /gene="endA"
                     /locus_tag="QGF01_03775"
     CDS             complement(1461..2000)
                     /gene="endA"
                     /locus_tag="QGF01_03775"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HJN20045.1"
                     /translation="MKSELIENRIIVWDIEHSKELFGNGYFGKPIGIPKPKVDEINVP
                     LVLDLIEGCYLQEISKIKIYKNKKRVSEKEMITICKKEYHNFDKKYLVYKDFREKGYI
                     VNPGIKFGSDFAVYQKGPGIDHAPYLVQVYNGNDPISSTDVVLAGRLASSVKKQFILA
                     IPKGKNNVEYLALDWWKPN"
     gene            complement(2002..2655)
                     /locus_tag="QGF01_03780"
     CDS             complement(2002..2655)
                     /locus_tag="QGF01_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HJN20046.1"
                     /translation="MYSGELEVQAKRKAIAVLQDEINRILNAARELSSLPELLVKKNK
                     TDVKAKLEQISSLEDEVENLRRKITRDVADVGGLIMNRENILNTAYTMDEIAGYITGI
                     AFKLSNIKVKTLTTEDFEKELTELIELVVDQVYKLNEIIRSLNTDSTKSVEFAQETQK
                     IEREIDIKYRNMSIKALDTISNTKELLLMKDIIEGIEEMADKCQEVSDSFILLALSI"
     gene            complement(2715..>3173)
                     /locus_tag="QGF01_03785"
     CDS             complement(2715..>3173)
                     /locus_tag="QGF01_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HJN20047.1"
                     /translation="DSLDLIVIGAYFGRGRRTGLYGTLLLGTYNPENDTFPSICKVGT
                     GFTDESLDQLYQILSNKVILKKNSRVVSEMEADVWLEPELVLEIVASEITLSPIHKTG
                     LNLVRKNSGFALRFPKFTGKIRYEKAVEDASTHDEVLALYKNQSKINHEK"
BASE COUNT          985 a          581 c          458 g         1149 t
ORIGIN      
        1 cagcctttat agtttcatca ctttcaccta acaatcccac aatttcatta tttgcagctt
       61 caacttcact tggtgatagg tcttcaaaac cgcttacacg tagaaattct gatgcagcct
      121 ttgggtaaac tgttttgtaa attctgtctg aatgaactgg accgggatta gttcctattg
      181 atataatacc cttttccgta agttcccatg acattgcctc tacaagtcta ttttttgctc
      241 cttgggatgc agtatatgga tctctgaatc tgtacggtct ttgttctaac ggcctttctt
      301 ctgcaaaaaa tgttgaaatt gtaataattt ttgccccttc tttcataact tttagtgcct
      361 gaactgaagt ccaaaaagtt ccagttagat gtatgtctac agtacttctg aattgatcaa
      421 gtggtgcatg tgcaaaacat gtcacagggc ccgagactcc ggcattgttt acaacaatat
      481 ctagattgac attttcactt tttaatgaat caaacatccc agtgacagct ttttcatcac
      541 taacatccac tccatcaaaa attttgatgg atgcatcact ttgtttttct ttgataattt
      601 cttctagcat ttttgcagtt tcttctagtg gttcctttct tcttcctaga attactgcgc
      661 ttgccccatt ttccacaaat tttttagtaa ttgcctgccc aatcccagta ccgcttccag
      721 taactaaaac agttttattt tgaaacttta gcatgagatc aaaaattagt ttatcatgta
      781 tttattttat tgctaatcat taatcaagaa caacaagaaa tgttaaatta tgtgctaaaa
      841 tcagatacga catggaagat tctgaacctg aaactattgt attaaaaaat tggcctgaaa
      901 aattttctga tatattaaat gagattggtg ttagtgcagt ccctaatgaa ataggtaaag
      961 gtgatgttga gaaaggagat tactatagta gatgcttcac tcaaaccact agaatgatta
     1021 ccaataaagg ctcattgaaa attactggta cagatattga tgctgtacat ataatacaga
     1081 agggatagaa atgcaagatc caaatccact tccatggggt gctcaggata gattccaagc
     1141 acattttatt gtccagaaag atattggtga agaattactg aaccttactg caagaactag
     1201 actacaaact acaggtcatt ttggttcaaa aaaaataact ggtgtaacat gggaaggagg
     1261 tcaactagct gatgagttaa acactgattc atcattgaat aaaatgattg ccaaacaatc
     1321 acttaacgac gcaataattt ttgttgatcc aacagataat ggggtacgca tttatggaaa
     1381 atggaaaaat agttataatt ttggaataac aaaagagtta tttgatattt acaataagat
     1441 tgcgggttac ataaagaaac tcaattaggt ttccaccaat caagagccaa gtattcgacg
     1501 ttgtttttgc cttttggaat ggcaagtatg aattgttttt taacactgct agctaatcta
     1561 ccagccaaca caacatctgt tgaagaaata ggatcattac cattgtaaac ttgaactaga
     1621 tatggggcat gatcaatacc aggtcccttc tgatatactg caaaatcaga accaaatttt
     1681 ataccaggat tcacaatgta tcctttttca cgaaagtctt tgtatacgag atatttttta
     1741 tcaaaattat gatattcttt tttacaaatt gttatcattt ctttttctga aactctcttc
     1801 ttatttttgt aaatcttgat ttttgatatt tcttgaagat aacatccctc gattaaatca
     1861 agaacaagtg gaacattaat ttcatccact ttgggttttg ggatcccaat cggttttcca
     1921 aaataaccat taccgaataa ttcttttgaa tgttctatat cccaaacaat aattcggttt
     1981 tctattaact cgcttttcaa atcaaatact aagtgctagt aaaataaacg aatctgaaac
     2041 ttcttggcat ttatcagcca tttcttcaat accttcaatg atatctttca ttaacaataa
     2101 ttcttttgta tttgatattg tgtccaatgc tttgattgac atattgcgat attttatatc
     2161 tatttcgcgc tcaatttttt gtgtttcctg agcaaattca acagatttag tagaatcagt
     2221 gtttaaactt ctgataattt catttaattt gtatacttga tcaacaacaa gttcaattaa
     2281 ttcagttaat tctttttcaa aatcttctgt ggttaatgtt ttcactttga tatttgagag
     2341 tttaaacgca attccagtta tgtagccagc aatctcatcc atagtatagg ctgtattcaa
     2401 gatattttct ctgttcataa ttaatccccc aacatctgca acatctctag taattttacg
     2461 tcgaaggttt tctacttcgt cttcaagact agaaatttgt tcaagtttag ctttaacatc
     2521 agttttattc ttttttacta gtaattctgg caaacttgat aattctctag cagcatttag
     2581 aattctattt atctcatctt gtaaaacagc tatcgccttt ctttttgctt gaacttctaa
     2641 ctctccacta tacataacgc taattaccta tagatcgctt ttctaattta tacctacttt
     2701 ttttgataaa tcattcactt ttcatgatta attttggact gatttttgta caaggctaat
     2761 acttcatcgt gagttgaagc atcttctact gctttttcat atctgatttt tccagtaaat
     2821 tttggaaatc tcaaggcaaa tccactattt ttccttacta aatttaatcc agttttatga
     2881 ataggactta atgttatttc tgatgcaaca atttctaata ctaattcagg ttcgagccac
     2941 acatcagctt ccatttcact tacaacccta gaattttttt taagtatgac tttgtttgag
     3001 agaatttgat ataactgatc caaactttca tcagtaaaac cagtgccaac tttacatata
     3061 ctaggaaaag tgtcattttc tggattatac gttcccaata acaatgtgcc atatagacca
     3121 gttctacgcc ctcttccaaa atatgcacct ataacaatta gatcaaggct atc
//
LOCUS       DASCPJ010000084        23347 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001376271, whole genome shotgun sequence.
ACCESSION   DASCPJ010000084 DASCPJ010000000
VERSION     DASCPJ010000084.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 23347)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23347)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23347
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001376271"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="QGF01_03790"
     CDS             <1..964
                     /locus_tag="QGF01_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HJN20048.1"
                     /translation="FLRVSGFEDLSPSEVEAANNEIVGLLGESDETIKAGIKSAAEKL
                     GKPETTLTNLLDKVKGIAEKIQKNTSTMIPDQQFLSQEQVATTVLTLADDAQAKILNG
                     KIIPGDRVFYPVKSHIRASVPKSEKNNLDGKRFILDEKAEVSSITSMIEEKGGKHEVS
                     NDYDLALFNTGNLPDFPKLTELPRDEWDKLVDQFINTPAQWTQSALKDFVPGGSEDPR
                     KFKDANGRIIIIGPALPAGKKISGHERAKVEVFRGALRPFATTVNQELSDVLKSNVRV
                     FLILPGTVDGKEPNNENIVNTINYLMSDEAISSSEVIFCPDETR"
     gene            complement(967..1722)
                     /locus_tag="QGF01_03795"
     CDS             complement(967..1722)
                     /locus_tag="QGF01_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HJN20049.1"
                     /translation="MFEEIWLIPLGFIAGILGSIVGLGGGIIIVPVLTFMGFSPTLAV
                     SNSLFAIFSNSVGATAAYAKQKRVEFSLGWKLGLMAVPGTILGAFISSEMSSEIFKIL
                     FALILISSASYIFLKRKIEEKPVDISRLLLVFSAGASFFAGIISSLFGIGGGLIFVPL
                     MVVALGISMKRAAPTSQFILMFASFSGLIVHSMLGHPDYYQALLLSIGAFAGGILGAR
                     LSLEIKENKLKIIVVIVLIAAAIKLIIDSTGVF"
     gene            complement(1724..2218)
                     /locus_tag="QGF01_03800"
     CDS             complement(1724..2218)
                     /locus_tag="QGF01_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HJN20050.1"
                     /translation="MVTRTNVLIPIAAAAVIVGIFGMLTFPTDVKLESVEFPIGTIKL
                     DDKILEVQIADTAPLHARGLMFQDEMSYDQGMLFVFDNPGLRPMWMLNMQFHLDIVWF
                     DENQNIIGIEKHVPPCKTTIEVVTCRENGVSHDNAKYVLELTSGFVDNFNITNKSKLE
                     LISI"
     gene            complement(2261..2611)
                     /locus_tag="QGF01_03805"
     CDS             complement(2261..2611)
                     /locus_tag="QGF01_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20051.1"
                     /translation="MEDPLKKHLAKLKAIKESVHRRAEMYSDLSKLDNKHTMRSLGAL
                     NKAPSPGKKFQKIGFIMLWIPEPTGITCAVGGPMIVAGRYLSKVYNDSKITDVGENTK
                     NTISEIHNFKQSLL"
     gene            2736..3197
                     /locus_tag="QGF01_03810"
     CDS             2736..3197
                     /locus_tag="QGF01_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HJN20052.1"
                     /translation="MKKDTKRLDSIKAANQSIDRKTHIRREDYLEIISELVGLKGYAT
                     TLDISRHMNVSPPSVTKMLQKLDKDGYLEYEKYHGINLTSKGNQVAEAIRQKHSTLLE
                     IFEILGIKKDIANQDAEGMEHYLNPKTVKILRKFLVFLKSNPKIIDEFHEF"
     gene            3284..3937
                     /locus_tag="QGF01_03815"
     CDS             3284..3937
                     /locus_tag="QGF01_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20053.1"
                     /translation="MSELDDLIRSGYGDKNKLRNIRETIKHDNFITANDKKYVSSLIT
                     KYLRNQPFEESDSFEEDYVDETKPTPQKLVSKDHPKTSLSVNTKLVVPIAIAAAVAII
                     AVIGFLAMNQGGVTDSVVISTPNNPLLINVDQSTYQKADIISISGDTNSHSKSVELSI
                     ENSNGVRIWSENITPKNDGVFSTLIIAGGGGWENGGDYLLKVVQGDLKNEAKFKFVA"
     gene            complement(3941..4171)
                     /locus_tag="QGF01_03820"
     CDS             complement(3941..4171)
                     /locus_tag="QGF01_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20054.1"
                     /translation="MGLIHTNHYLDFPVDAWKQISSNPTVFEAIVDNVVLELLDTGHR
                     EQKIVFKNGGKIKYIRSIGKYRLSWDDEDLVS"
     gene            4295..4864
                     /locus_tag="QGF01_03825"
     CDS             4295..4864
                     /locus_tag="QGF01_03825"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HJN20055.1"
                     /translation="MNSNLTIKSINEKIVKCTKCNLCKNRKNAVPGIGNEHADIVFVG
                     EAPGRSEDKYGKPFIGSAGKKLDEALKYAGLTREEVYITNIVKCRPPKNRIPTDHEKI
                     MCSMYLEKELLIIDPKIVCLMGNTAYHSILNGKEISKNHGKVIDKDDKTYFVTFHPAA
                     MIYNKKLDAVFKNDIKKLVKIVKNLKKND"
     gene            4857..5423
                     /locus_tag="QGF01_03830"
     CDS             4857..5423
                     /locus_tag="QGF01_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HJN20056.1"
                     /translation="MIDSKILPRGFYNNDTVKVAKNLLGKNLVRVVGKKILSGIITET
                     EAYKSKHDPASHAVNRITDRNKAMFGQVGRAYVYFTYGNYFMLNAVARGKNNEAGAVL
                     IRSIYPQDGMKIMLKNRKNTDIRNLTNGPGKLTQAMQISISQYNTDLTKKSAIFITEG
                     IKPKKIFKKSRVGITKGIDKLWNFSFNI"
     gene            complement(5420..6043)
                     /locus_tag="QGF01_03835"
     CDS             complement(5420..6043)
                     /locus_tag="QGF01_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HJN20057.1"
                     /translation="MDLFPFAPEIGYIGLSLVSFFGSLIPFVPIPSFVLLATMAVGEQ
                     FNIHLLALFAALTSTVAKQIIFYLSYGGRKIISEKTKNRMKPFQKLVRRYGATAAFVA
                     AATPIPDDIVYIPLGLAKYNPKKFFVATLLGKIVLCYIIVLISHYMGLSILEPILEDI
                     ENPAPVYFGIILFGVIMTVTVILLLRLDWAKILGRIAPWTLEDDENN"
     gene            complement(6359..7294)
                     /locus_tag="QGF01_03840"
     CDS             complement(6359..7294)
                     /locus_tag="QGF01_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJN20058.1"
                     /translation="MVKLYVSDLSVNYETSSGDVHALENVDFQLEDNESIGIAGESGC
                     GKSTLGLSIIKMIQGGKIIAGEITLGEKSILNMTESEFDDIIRWKEISMVFQGAMNSL
                     DPVFTIEQQFDQILKQHNSTDDFKKTMHNSLDTVSLSHSILKKYPHELSGGMKQRVVI
                     AMALLLEPKFVIADEPTTALDVLIQAQIINLLKSLKKKDVSIMLISHDLAILSEIVEK
                     IGIMYGGKMVEFGSSTEIYREPKHPYTKGLLDSIPTLHGERKLKFIPGNPPNLLSPGS
                     GCRFFDRCPEAMDKCKKNPPKIKTDSGYVLCWLYE"
     gene            7364..8659
                     /gene="aspS"
                     /locus_tag="QGF01_03845"
     CDS             7364..8659
                     /gene="aspS"
                     /locus_tag="QGF01_03845"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HJN20059.1"
                     /translation="MIYVKFNRTHYIEDVKSSMNGQEVILGGWVEDLRKMGKMTFLTL
                     RDVTGITQIILTDDIIKSTEDITRQSVVRITGKIQDTRARDFECEIKASEINLLAKAI
                     HPLPIDPIGRLESHIDNRLNSRALDMRNQKTASIFKIRHHTLAALRKSFIEKKFIEIT
                     TPKIIGSASEGGANLFSLDYFGKQAYLAQSPQLYKEQMTIGLERVFEISSFYRAEKSH
                     TGRHLSEFTSIDMESAFMSYTDVMDVLEDLVVSTFKYVSENCKKEQETIGNKTIIPNN
                     PFKRITYSHALEELNKKDAKLEFGDDLLDSHLRILGEIYPEFYFLTDWPLKIKPFYIS
                     EKEDNAELSESFDLQFGYLELSSGGTRLHNPEKIKMRLSEQNLDPAKFSEHLQAFDWG
                     MPPHAGWGLGLERLLTVIIGIDNVREVILYPRDPERLKP"
     gene            complement(8648..9667)
                     /locus_tag="QGF01_03850"
     CDS             complement(8648..9667)
                     /locus_tag="QGF01_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HJN20060.1"
                     /translation="MTKKYQISLITGDGIGPEISESAFSILQEIHDNMGIKFEIKKLE
                     AGDTVLKNTGNALSEETFEIIKNSDACLKAPVGESAKDVIVYLRRMLDLYANIRPAKS
                     YPNTPSLRDDIDLVIVRENTEDLYTGEEFDIGDTSVAMRIISEKASKRIAEYAFKTAT
                     QRKMKKVTCVHKSNVMKKTDGLFARTCSKVADNFPNIKFEEMYVDACAMNLIRQPQLF
                     DVIVTTNLFGDILSDESAQVVGGLGMAPAANIGDKFGLFEPVHGAAFDIAGKNIANPT
                     SFILSIKMMLNWIGNKYADTKCIEIAQKLENVVYDVVKNNIKTKDTGGDKTTVEFTKE
                     IISRL"
     gene            complement(9683..11194)
                     /locus_tag="QGF01_03855"
     CDS             complement(9683..11194)
                     /locus_tag="QGF01_03855"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJN20061.1"
                     /translation="MKVRIFDTTLRDGEQSPGVALSPENKLSIAKKLDELGVDAIETG
                     FPVISQGEQQAVKMITEANLSAELCGLARTNKKDIDAVVDCGLKYVHTFIATSDIHLE
                     HKLHLSQDEALEKAVESVEYAKSRGLQVEFSAEDATRTDRNFLKKIFTAVTKAGADRI
                     DIPDTVGYSTPQYIAEITKDAIEATKLPISMHCHNDFGLAVANAISGIQAGAQCAHVT
                     INGIGERAGNASLEELVMALQCLKFDQSWETNIKTKLLYDTSKYVSKLAGMPVQPNKA
                     IIGENAFGHESGIHTHGVLSNPLTYEPISPEIVGRTRWLQVGKHAGVHGINAMLQEYG
                     IEPNNDQTRQILEKVKSIGDQGKHVTDVELFSIANEVIGDTKLKRLVKLSGFSVSTGI
                     GNMPYAFVKLIIDGNEHTATDHGVGPVDASLNAIQKATGKISEIKIKDYNLASISGGS
                     NALCEVTVKLEDATGNMISSKSIGEDIVITSVQAVIDGINRLMLKKSLREKKV"
     gene            complement(11205..11672)
                     /gene="ilvN"
                     /locus_tag="QGF01_03860"
     CDS             complement(11205..11672)
                     /gene="ilvN"
                     /locus_tag="QGF01_03860"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HJN20062.1"
                     /translation="MWAILSIRVENKPGILFKVTHLFRSRNFNIDSISVGVMENPEFS
                     KMTITTVGTEKQIAQIVKQLDKMIDTIEVKRLDENESVYKELALFKIKLSEASDSIEI
                     NKLADTYGAKVHNEQKDSLIVELTSTPDKIKTFEEVLKPFGILDSARTGVTAL"
     gene            complement(11681..13381)
                     /gene="ilvB"
                     /locus_tag="QGF01_03865"
     CDS             complement(11681..13381)
                     /gene="ilvB"
                     /locus_tag="QGF01_03865"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HJN20063.1"
                     /translation="MNDKMEVMNGGKALMKALEKEGVKEVFGLPGGANLPMYDELYKS
                     NIRHILVRHEQSAAHMADGFGRVSRKPGVCFATSGPGATNLLTGIATAQADSAPMIAV
                     TGQVPVNMIGRDAFQESDIIGMSNPCVKYAFQPRTPQEIPEVVKKGFYIAETGRPGPV
                     LLDIPKDVQQNEAEITFPNEVRIPGYHPWTDVDITNIERSIDLLLSSEKPIILAGGGT
                     IISSAFAELQSIAEMLMIPVVTTFKGKGSFPENHPLSLGPIGMHGHAEANKIMSEVDC
                     VLAIGTRFSDRSVGTFEEFEKNLKIIHMDVDPAEIGKNQTTQVAVVGDVRASLRVMLK
                     ILAKKVLKKSDGNKWLKHVNEVKDYWRENLKLHPGEMSAAKILRKLREVLPKESIVTT
                     EVGQHQMWASLFFDAIHPGTFFSSTGLGTMGWGFPAAIGAKVARPDVPVLDIAGDGSF
                     NMTENSLATSVLEDIPVIVFLINNFSLGMVAQWQRTFYERRMIGIDLKKCPDYVKLAE
                     SYGAQGIRAQSLDELGKAVKVGLNSEVSTVIDIPIDPEEDVLPFVAPGTSLKDMILPS
                     "
     gene            13596..14000
                     /locus_tag="QGF01_03870"
     CDS             13596..14000
                     /locus_tag="QGF01_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HJN20064.1"
                     /translation="MSDEQEILEVIRTFFEAGRTKDFSILSKIQLNSQKFSSFSDIPP
                     YGLKDYSESIALEELRFSSITDYNYEIKNEKTNIFDSVGISTFTLEQKGMLVDSQTYT
                     GQYMTINGRATFVLIKKPTWKIVHYHLDKISN"
     gene            complement(14011..14826)
                     /gene="lsrF"
                     /locus_tag="QGF01_03875"
     CDS             complement(14011..14826)
                     /gene="lsrF"
                     /locus_tag="QGF01_03875"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HJN20065.1"
                     /translation="MDWGLKNRLAHIIKPNDNRALMLAVDHGYFLGPTEKLEIPKKTI
                     GPLLKYCDSLMLTRGVQRTSVNPTYPVPIVLRVSGGASIIGQDLSNEQITVSIKDAIR
                     LNASALAMSIFVGAKHEHQTIVNLSKLVNDANEYNLPVLAVTAVGKEIGQKDARYLSL
                     ACRIAAEHGAHIVKTYYCDNFERVVDSCPVPIIVAGGKKIPERDALELAYNSIKGGAV
                     GVDMGRNIWQSENPVPMIRAVHNIIHGNANVDEAYELYKKLCQPKPKSPKTKK"
     gene            complement(14869..15903)
                     /locus_tag="QGF01_03880"
     CDS             complement(14869..15903)
                     /locus_tag="QGF01_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HJN20066.1"
                     /translation="MKAAIVKSDSNIEIKNMIKPSLGSDDILVKMHACGICGSDVEKV
                     FGKYGQASMKLGHEPAGTITEVGSEISDFSIGDRVFTHHHVACYSDDCHECNHGNETM
                     CKKYYESNLEPCGLADEYVVPGWNVKHGGVLKIPDNVSFEEAAMIEPLACCIRAWNKF
                     THKTNDSVAILGVGPTGIMHVLLAKFYGLKEVFCLDLNDYRLDFAQKLETITIHSDDP
                     TASEIMKSNLQHPFGVDVVIVATGNLKALQDAVTFVRKGGTIVMFGVPSKGATVELDM
                     SKVYSKSITIVNSYAASDMDTRDALEKISSKQINVTQLITHKYKLNECQKAFEHAKSG
                     ENAMKIIISE"
     gene            16002..16556
                     /locus_tag="QGF01_03885"
     CDS             16002..16556
                     /locus_tag="QGF01_03885"
                     /inference="COORDINATES: protein motif:HMM:NF023043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HJN20067.1"
                     /translation="MAKIGTFILLGLIIGSISFSLFLFLDTQTITVISESGGAVIAGD
                     VKFYVEHIGNHEILKKTDEYSEAEKNLVQKGLDRSEIPEGVYFQIQINAHNTGDETVR
                     VTGGQFYLYDTDNEKYEAVFIGYGEEELSVIDLKPNETIVVTTQFDIPYDEEMKYTIG
                     IIPNKYGLQDAQERIFVCVKQCEV"
     gene            complement(16557..16865)
                     /locus_tag="QGF01_03890"
     CDS             complement(16557..16865)
                     /locus_tag="QGF01_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20068.1"
                     /translation="MNKSSKKYNSVLNEKRIKLHVFEPSNRKIWTVVGSDREYWLDPD
                     LDFCSCPGYYFTKKNNEKNCYHLDSLKTINHATDIESVTFSDTEYRDFLSGLLSDLKK
                     "
     gene            16900..17646
                     /locus_tag="QGF01_03895"
     CDS             16900..17646
                     /locus_tag="QGF01_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HJN20069.1"
                     /translation="MVKTRIVPNYPALLSTGSEKNLIITDLHLGLEGNLSLNKVFVEK
                     NVTVKKTIIEIEKLLKITKPDSLILLGDIKSGIKSITKTEWESIPMFFDKLKNRVNLI
                     LVPGNHDSNIEKLVPKEVNIASPKGIIIDEILFTHGHTLPTENYGNVSKIVMGHLHPV
                     FFQKESIINGKRVWISIKCSKDEIFPSQTGDVELIIVPAFNKYFHMTKKKFYKKSISP
                     IIEKINTTKAKIITLDGTIIGDEKLLPNLI"
     gene            complement(17643..20885)
                     /gene="carB"
                     /locus_tag="QGF01_03900"
     CDS             complement(17643..20885)
                     /gene="carB"
                     /locus_tag="QGF01_03900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HJN20070.1"
                     /translation="MPKNESLKKILVLGSGAIKIGEAGEFDYSGSQCLKAIHEDGLKS
                     VLINPNIATIQTDTRFADKVYLQPVTPQYVESVIESERPDSIMLSYGGQTALNCGVKL
                     DEMGILQKYDVKVLGTQIPGIMATEDRQKFKDNMQKCGVPVLKSKTVHTFEGAKKVAE
                     ELGYPVIVRVAYTLGGKGGGVAHNEIELHEIAERGIAASLVGQILVEEYIGHWKQIEY
                     EVMQDYGGNNVIVCNMENVLSMKVHTGDNIVVAPSQTINNHEYHTLRSAAIRATKHVG
                     IVGECNIQYALDSDSDRYVAIEINPRLSRSSALASKATGYPLAYMSAKIGLGYSLPEL
                     VNRITKSTTACFEPSLDYIVCKHPRWDFEKFELVNRKLGVTMKSVGEVMAVGRTFEES
                     LQKAIRMLDIGKDGLVLNRENGQKFDVEDIEDHLQHHDDFILYFVAAALKKGITVQKI
                     YDLSSIDPWFIEKIKNIVETEEKLKQNELEKPLLQEAKKLGFSDNQIARAKDMQSTEV
                     RKIRKDYNIIPSVKQIDTLAAEWPAKTNYLYLTYGGETNDIEINESDKGVIVLGAGPY
                     RIGSSVEFDWGTVNMVWGLQENGEKNVSVVNCNPETVSTDYDICSRLYFEELTLERIL
                     DIAEFENPKGIVTSVGGQTANNLTPNLAKNGINLIGTSAENVDRAENRSKFSEILDNL
                     HIKQPLWQAFSNITDAKNFALDVGYPVLVRPSYVLSGAAMKVVWSQEELKEFVTNAAV
                     VSPDYPVVITKFMLDSLEVDVDGVSDGKNVVIGAIVEHIDSAGVHSGDAMMCIPPWRI
                     SNRIIENITDSTVRIAREFKIKGPFNLQFLINNDQEYVIELNIRASRSMPFVSKLVRM
                     NLISLASKAVLGKQLPHIPYDEWKKIPIHGIKVPQFSFMQLDGADIVLGVEMQSTGEA
                     ACFGDSFYDALSKGLTSVGYNLPKSGSALVTIGGVKNREKLLQTSALLQKLGFKILAT
                     ETTAEFLNEKLGNVEIVHKISEPDRKPNISDLLYQKKIDFIINIPSTSTIEKYVGMLY
                     DEYQIRRKSLELGIPVLTTIELAESFVKTLEWLQNNTPTKKALEPYEDYN"
     gene            complement(20890..21987)
                     /gene="carA"
                     /locus_tag="QGF01_03905"
     CDS             complement(20890..21987)
                     /gene="carA"
                     /locus_tag="QGF01_03905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HJN20071.1"
                     /translation="MILSDGTILDGMGFGFSTTVFGEIVFNTGMVGYTEALTDPSYNG
                     QILTLTYPLVGNYGIPDLTEVDEDGISKFFESDMMQIRGLVVHELSQTASHWNLKMTL
                     DEWMYNEKVPGISGIDTRAITKKITSSGVMMAALVVSDEQIDVEKIKKELTNVTKYNS
                     EKFMDIVSTKEEKIYGDDNQSVVVIDTGAKNAIVRNIRELGYKAIVVPWNTPFNKVMS
                     YNPKGVVLSSGPGDPQNCPETIDTAKKLIENNIPTLGICLGAQIIGIAGNTETYKLKY
                     GHRGQNKPCINLETNQVYVTSQNHGYGIKPESLEQSDFKLWFSNADDNTVEGIKHKTQ
                     NCIAVQFHPEAAPGPFDCKYVFDELKKLMEK"
     gene            complement(22109..23305)
                     /locus_tag="QGF01_03910"
     CDS             complement(22109..23305)
                     /locus_tag="QGF01_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJN20072.1"
                     /translation="MNLAPQELERNASKSASDAIKYDSQGARGMAIAHYEKAIQSLSK
                     LMKLYPDSKLNTIYAERLKSYQNRIKALQSLQGNDFEPAVDPKATPEEQQSNLGKARQ
                     DSFEEMIMKEKPDINWRDVVGVDEAKNALRESIVWPTKRPDLFPLGWPRGILLYGPPG
                     CGKTILAAATANELDGYFINVDASSMMSKWLGEAEKNVAKLFRMARSYAEREDKPVIL
                     FIDELDSLLGERTNEIGGEVRSRNQFLTEIDGISNKGKSIKLYVIGATNKPWSLDNPF
                     LRRFQKRIYVSLPTIDARTKLFDLYTHQLKMDSRVRTRSLATIFDGYSASDIKDICQA
                     SQMKVVNELFCSPQFKEPVAGENPTQPRDLTLKDFKEILERRKPSVSVEMIRAYYKWS
                     EQFKAL"
BASE COUNT         7766 a         4181 c         3625 g         7775 t
ORIGIN      
        1 atttctgcgt gtaagcggtt ttgaagacct atcaccaagt gaagttgaag ccgcaaacaa
       61 tgagattgta ggattgttag gtgaaagtga tgaaactata aaggctggaa taaaatcagc
      121 tgcagaaaag ttaggtaaac cagaaactac acttaccaat ttactggata aagtaaaagg
      181 cattgcagaa aaaattcaaa agaacacctc aacgatgatt ccagatcagc aatttttatc
      241 gcaagagcaa gttgccacaa cagttctaac acttgcagat gatgcacagg caaaaattct
      301 caatggaaaa ataattcctg gtgacagagt tttctatcct gtcaagtcac atattagagc
      361 atccgttcct aaatctgaaa aaaataactt ggacggaaaa cgatttatct tagacgaaaa
      421 agctgaagta agcagtatta catcaatgat agaagaaaaa ggtggaaagc acgaagtttc
      481 taatgattat gatttagctt tgtttaacac tggtaatctt ccagattttc ccaaactaac
      541 agagttaccc agagatgaat gggataaatt agttgaccaa ttcattaaca cgcctgccca
      601 atggacacag tctgcgttga aggatttcgt accaggtggt tctgaggacc caagaaaatt
      661 caaagatgca aatggaagga tcattataat cggtcctgct cttcccgctg gtaaaaaaat
      721 cagtgggcat gaaagggcaa aagttgaagt tttcagaggt gcccttagac catttgcaac
      781 tacagtcaac caagaattga gtgatgtact gaaatcaaat gtaagggtat tcttaatttt
      841 gcctggaaca gtagatggaa aagaaccaaa caacgaaaat attgtaaata caattaacta
      901 cttgatgtca gatgaagcaa tatcaagttc ggaagtaatt ttctgcccag acgaaacaag
      961 ataacactag aaaacacctg tagaatctat aattagctta attgcagccg cgataagtac
     1021 gattaccact ataattttca atttattttc cttgatctca agtgataatc gagcacctaa
     1081 aattcctcca gcaaaggctc caatcgacaa caataatgcc tgataataat caggatgacc
     1141 caacatggaa tgaacaatca atcccgaaaa agacgcaaac atcaagatga attgtgatgt
     1201 tggtgcagca cgcttcattg agattccaag tgcaactacc atcaatggta caaagatcaa
     1261 accacctcca ataccaaata gacttgatat tattccagca aaaaagctag cacctgcaga
     1321 aaaaacaagt aaaagacgag atatgtctac aggtttttcc tctatttttc ttttgagaaa
     1381 tatgtatgat gctgaagata tgagaattaa tgcaaacaaa attttgaaaa tttctgatga
     1441 catctcagaa gagatgaatg caccaagtat ggttccagga acagccatta aaccaagttt
     1501 ccaacctaac gaaaactcta cacgtttttg tttagcatat gcagctgtgg ctccaacaga
     1561 attgctaaag atagcaaaca aactgttgct tacagccagt gtaggagaaa atcccatgaa
     1621 tgttaataca ggaacaataa ttattcctcc acccaaacca actattgatc ccaatattcc
     1681 tgcaataaat cctaaaggaa ttaaccaaat ttcttcaaac atattaaatc gatatcagtt
     1741 caagtttgga tttatttgta atgttaaagt tgtcaacgaa accagaagta agttccaata
     1801 cgtattttgc attatcatgt gaaactccat tttctctaca tgttacaact tctatagtgg
     1861 ttttacaagg cggaacatgt ttctctatac caattatatt ctgattctca tcgaaccaga
     1921 caatatctaa atgaaattgc atatttagca tccacatagg acgcagccca ggattatcaa
     1981 aaacaaataa cattccttgg tcatatgaca tttcatcttg gaacattaat cctcttgcat
     2041 gtaatggagc agtatcagca atttgtacct ctaaaatttt atcatcaagt ttgattgttc
     2101 caattggaaa ttcaacagac tctagcttaa catctgtagg gaaagtaagc atgccaaaaa
     2161 ttccaacaat aactgcagcc gcagcaattg ggattaaaac atttgttctt gtaaccatat
     2221 attacaataa tatttctctg cataaaaact aaaagaaatc ttataataat gattgcttga
     2281 aattatgaat ttcagagatg gtatttttgg tattttctcc tacatcagtt atttttgaat
     2341 cattgtatac tttgctaaga tatctaccag ctacaatcat tggtccccca actgcgcatg
     2401 tgattccagt aggttctgga atccaaagca tgatgaaacc aattttttgg aattttttcc
     2461 caggtgatgg agctttgttt aatgcgccca aagaacgcat agtgtgctta ttatctaatt
     2521 tactcaaatc ggagtacatt tcagccctac gatgaacaga ttcttttatt gcctttaatt
     2581 tggcaagatg ttttttcaaa ggatcttcca tatctaatat atgaagaaaa ttacataaag
     2641 aacaaagtaa aatctacgtc catcaacggt caaaaattga ttacaagaca ttctaacagg
     2701 cagaaaatta tactagttca agcataaatt aaagaatgaa aaaagacacc aaaagactag
     2761 attcgattaa agcagcaaac caatctatcg atagaaaaac acacatcaga agagaagatt
     2821 atctagagat tatttcagaa ttggttggtc taaagggata tgcgacaaca ttagatatat
     2881 caagacacat gaatgtcagt cctccaagcg tcacaaaaat gctacaaaaa ttagataagg
     2941 atggatacct tgaatatgaa aaatatcatg gaatcaattt gacgtctaag ggtaatcaag
     3001 ttgcagaagc aattcgtcag aaacatagta cattattgga aatttttgag attttaggaa
     3061 taaaaaaaga tattgcaaat caagatgcag aaggtatgga acattaccta aatccgaaaa
     3121 ccgtcaaaat attaagaaaa tttcttgtat ttttgaaatc aaacccaaaa attattgatg
     3181 aatttcatga attttaaata aattgttcat tttcacgcaa ttattgattc catgaatagt
     3241 gaattttaat taaagagtga atatgagata aaatatgaca ttcatgtctg aacttgatga
     3301 tttgatacga tctggttatg gggacaaaaa taaattaaga aatatcagag aaaccataaa
     3361 acatgataat tttatcactg ctaatgacaa aaaatatgtc agttcgttaa ttaccaaata
     3421 cttaagaaat caaccgtttg aggaatctga ttcatttgaa gaagattatg ttgatgagac
     3481 gaaacctaca ccacaaaaat tagtttcaaa agatcatcct aaaactagtt tatcagttaa
     3541 tacaaaacta gttgttccta ttgcaatagc tgctgcagtt gcaataattg cggtaatagg
     3601 atttttagca atgaatcagg gtggtgtgac agattcagta gttatctcaa caccaaataa
     3661 tccattactg ataaatgttg atcaaagtac ctaccaaaaa gctgatatta tttctatatc
     3721 aggtgatacc aactcccata gtaaatcggt tgaattatca atagaaaact caaatggagt
     3781 aagaatatgg agtgagaata ttacaccaaa aaatgatggt gtattttcga cacttatcat
     3841 agctggtgga ggtggatggg aaaatggtgg tgattatcta ttaaaagtag tacagggtga
     3901 tttaaaaaat gaagccaagt tcaagtttgt tgcataaaaa ctaagatact aaatcttcat
     3961 catcccaact taatctgtat tttccaatag aacggatata ctttatttta ccgccatttt
     4021 tgaaaacaat tttttgttcc ctatgtcctg tatcaagcaa ttctagtact acattatcaa
     4081 caatcgcttc aaaaactgtt gggttactag atatttgctt ccaagcatct actggaaaat
     4141 ctaagtaatg atttgtatga atgagaccca tactatacaa taatttaggt acctaaagac
     4201 tttacaatat ttgaatgtca tagcattcac aataatttta actatgaaca gatttttaca
     4261 caagatttag gaatgtgtga ttaaattata atttatgaat agtaatctaa caattaagag
     4321 tattaatgaa aaaatcgtca aatgtactaa gtgtaattta tgtaaaaata gaaaaaatgc
     4381 tgttcccggt ataggaaacg aacatgctga tattgtgttc gtaggagaag ctccaggaag
     4441 atctgaggat aaatatggta aaccattcat tggttcagct ggtaaaaagc ttgatgaggc
     4501 actaaagtac gctggactta ctagagaaga agtatacatc acaaatattg ttaaatgtag
     4561 acctccaaaa aatagaattc ctactgatca tgaaaaaata atgtgtagta tgtatttaga
     4621 aaaagaactc ttaatcatcg atccaaaaat agtttgctta atgggaaata ctgcctatca
     4681 ttctatatta aatggtaaag aaatttcaaa aaatcatgga aaggtaattg ataaagatga
     4741 taaaacatat tttgttactt ttcatccagc tgcaatgatt tataataaaa aacttgatgc
     4801 tgtctttaaa aatgatataa aaaaattagt aaaaattgta aaaaacttga aaaaaaatga
     4861 ttgattcaaa aattttacca agagggtttt ataataacga tacagttaag gttgcaaaaa
     4921 atcttctagg gaaaaattta gtaagagttg ttggaaaaaa aatattatct ggtattatta
     4981 cagaaacaga agcttacaaa agtaaacatg atcctgccag tcatgcagta aatagaatta
     5041 cggatagaaa caaagccatg tttggtcagg ttggaagagc atatgtttat ttcacatatg
     5101 gtaattattt tatgcttaat gctgtagcta gaggtaaaaa taatgaagca ggtgcagtgt
     5161 taattcgttc tatttatcct caagatggta tgaaaattat gttgaaaaat agaaaaaata
     5221 ctgatattcg aaatcttaca aatgggcctg gaaaattaac ccaagctatg caaatttcta
     5281 ttagtcaata caatactgat ttaacaaaaa aatctgctat tttcatcaca gaaggtataa
     5341 aaccaaaaaa aattttcaaa aaatcaagag ttggtatcac aaaaggaatt gataaattat
     5401 ggaattttag ttttaatatt taattattct cgtcatcctc aagtgtccaa ggagctattc
     5461 tgcctaaaat ttttgcccaa tctaatctca aaagtagaat tacagttaca gtcataatca
     5521 caccaaataa aataatgcca aagtaaactg gtgcgggatt ttctatatcc tcaagaattg
     5581 gttctaaaat tgatagcccc atataatgtg aaattaaaac gatgatataa catagaacga
     5641 tttttcctaa taatgttgca acaaaaaatt tttttggatt gtattttgct aatcctaagg
     5701 gaatgtaaac tatatcatca ggaataggcg ttgctgctgc cacaaaagct gcagtagcac
     5761 cataacgcct gactaatttc tgaaatggct tcatgcggtt tttagttttt tcagaaataa
     5821 tttttcggcc accataactt aggtaaaata ttatttgttt agcaactgtg gatgttaatg
     5881 ctgcaaataa agctaataaa tgaatgttaa attgttcacc aaccgccatt gttgctaata
     5941 aaacaaatga tggtataggt acaaaaggaa ttaatgaacc aaaaaaacta acaaggctta
     6001 atccaatata accgatttca ggagcaaaag gaaaaagatc caaaaggtcc aatatgtaca
     6061 aattatacaa tgagtattta aggcgatcaa aaaaaaaaat agtaaatgcg atcagatttt
     6121 ttaatgatta tgccgtatat gaaatatgta ctctacatgt tcacaatgtg gtcgattaat
     6181 atctagacct gaaaccaaat actgttcaga tgaatgcgaa acaaaacaaa ataccaaatg
     6241 aaatatcgat taaataatac tctctaaatc aattccattc tcattataga aaagttaata
     6301 attttagatg ctcatgatta caaatttttg ttaagatgaa tttgaatgcc acaacaaatt
     6361 attcataaag ccaacataaa acatatccag aatctgtttt aatttttggt ggattttttt
     6421 tacatttatc catcgcttct ggacatctat caaaaaatct acatcctgat cctggagaaa
     6481 gtaagttagg tggattacca ggaataaatt ttagttttct ctctccatgt aatgttggta
     6541 tggaatccaa taaccccttt gtgtatggat gctttggttc acgatatatt tctgttgacg
     6601 aaccaaactc aaccattttt ccgccataca ttatacctat tttttctaca atttcagata
     6661 agatggcaag gtcatgtgaa attaacataa ttgacacgtc tttctttttc aaacttttca
     6721 ataaattgat gatttgtgct tgaatcaata cgtctaatgc tgtagttggt tcatcggcta
     6781 taacaaattt aggttcaagt aacaatgcca tagcgataac tactctttgc ttcataccac
     6841 cactcaactc atgagggtat ttttttaaaa tagaatgtga aaggcttact gtatctaatg
     6901 aattatgcat ggtcttttta aaatcatcag tagaattgtg ctgctttaga atttgatcga
     6961 attgttgctc gatagtaaaa actggatcca atgaattcat agcaccctga aacaccatcg
     7021 aaatttcttt ccaacgtatg atgtcatcaa attcagattc tgtcatattt aatatggatt
     7081 tttcaccaag tgttatttcg cccgcaataa tttttccacc ttggatcatt tttatgatcg
     7141 ataaacccaa agtactctta ccacatccac tttctcctgc aataccaatt gattcattat
     7201 cttctaattg aaagtcaaca ttttctaaag cgtgaacatc tccagatgat gtttcataat
     7261 tgaccgatag atctgacaca tacagtttta ccattaaaaa taataataaa cgctctattt
     7321 tattttttca agaaaggata tattcaatgg atcataatta aaaatgattt acgtgaagtt
     7381 taatcgaacc cactatattg aagatgtaaa gtcgtctatg aacggccaag aggtgattct
     7441 tggaggttgg gtggaagatt taagaaaaat gggtaaaatg acatttctta ctctacgcga
     7501 cgtaacaggc attactcaga ttatacttac agatgatata ataaaatcaa ccgaggatat
     7561 aacacggcaa agtgttgtga gaatcactgg taaaattcag gatacaagag ccagagattt
     7621 tgagtgtgaa attaaggcta gtgaaattaa tttattagca aaagctattc atcctttgcc
     7681 cattgatcct atcggtaggc ttgaaagtca tatagacaat aggctaaact ccagggcact
     7741 agatatgcgt aatcaaaaaa cggcatcaat cttcaaaatc agacatcata ctttagcggc
     7801 gttaagaaaa tcattcatag aaaaaaaatt catagaaatc actacgccta aaattattgg
     7861 tagtgcgagt gaaggtggtg caaacctatt ctcattagat tattttggaa aacaggccta
     7921 tcttgcacag agtccacagt tgtataaaga acaaatgacg attggtctag aaagagtgtt
     7981 tgaaatatct tcattttaca gagcagaaaa atcacataca ggtagacact tgagtgaatt
     8041 tactagtatt gacatggaat ctgcatttat gagttataca gatgttatgg atgttttaga
     8101 ggatcttgtt gttagcacat tcaagtatgt ctctgaaaat tgtaagaaag agcaagaaac
     8161 aatagggaat aaaactatta ttccaaacaa tccattcaag agaatcactt attcacatgc
     8221 tttagaagaa ttgaataaaa aagatgctaa attagaattt ggagatgatc ttttagattc
     8281 tcatctaaga atattgggag aaatatatcc cgaattttat tttctaacag attggccact
     8341 caagatcaaa ccattctaca taagtgaaaa agaagataat gctgaacttt ctgaatcatt
     8401 tgatctacaa ttcggttatc tagaattatc atctggtgga accagattac ataatccaga
     8461 aaagatcaaa atgaggttaa gtgaacaaaa ccttgatcct gcaaaatttt cggagcatct
     8521 acaagcattt gattggggta tgccacctca tgcaggttgg ggactaggtt tagaaagatt
     8581 acttacagtg attataggaa ttgataatgt gagagaagtt attttgtatc caagagatcc
     8641 agaaagatta aagccttgag ataatttctt tagtgaactc tacagtagtc ttatcgccac
     8701 cagtatcttt tgttttgatg ttatttttta caacatcata aacaacattc tctaattttt
     8761 gggcaatttc aatacatttt gtatcagcat acttatttcc aatccaattt aacatcattt
     8821 tgattgaaag tatgaatgaa gttggattag caatattttt tcctgcaata tcaaatgctg
     8881 ccccatgaac aggttcaaat aatccaaatt tatcgcctat atttgcagca ggagccatac
     8941 ccaaaccacc cacaacttga gctgattcat cagaaagtat atcaccaaat aaattagttg
     9001 taacaatcac atcaaataat tgtggttggc gaattaaatt catagcgcat gcatcaacat
     9061 acatttcttc aaatttgata tttggaaaat tatcagcaac ttttgaacat gtacgtgcaa
     9121 ataaaccgtc agtttttttc atcacatttg atttatgtac acacgtaact ttcttcattt
     9181 ttctttgggt tgcagttttg aaagcatact ctgcaattct tttagatgct ttttctgaaa
     9241 ttattctcat tgcaacagat gtatcaccta tatcaaactc ttcacctgta tacaaatcct
     9301 cagtgttttc acgtacaatt accaaatcaa tatcatcacg caacgatggt gtgttaggat
     9361 atgattttgc tggtcgaata tttgcatata gatctaacat acgtcttaaa tatacaatca
     9421 cgtcctttgc actttcccca actggtgcct ttaaacatgc gtcagaattc tttattatct
     9481 caaatgtttc ttctgataat gcatttccag tattttttaa aactgtatca ccagcctcta
     9541 gtttttttat ttcaaacttg atacccatat tatcatggat ctcttgtaaa atactaaatg
     9601 cggattctga aatttctgga cctattccat ctccagtgat taatgatatc tgatattttt
     9661 tggtcattct actatacaat gattatactt ttttttctct taatgacttt ttcaacataa
     9721 gtctatttat tccgtcgatt acagcttgaa cactagttat gactatatcc tcaccaattg
     9781 atttagagga aatcatattt cctgtagcat cttcaagttt cacggtaacc tcacataatg
     9841 cattagaacc tcctgatata gatgctaaat tataatcctt aattttgatt tccgagattt
     9901 tgccagtagc tttttggatt gcgttgagag atgcatctac tggtccaact ccatgatctg
     9961 tagctgtgtg ctcgttacca tcaatgatca atttcacaaa tgcatatggc atattcccaa
    10021 ttccagtaga aactgaaaat cctgatagct tcactaagcg ttttaattta gtatcaccaa
    10081 tcacttcatt tgctatggaa aataactcaa catcagtaac atgttttccc tgatctccaa
    10141 ttgattttac tttttcaaga atctgtcttg tttgatcatt gtttggctct ataccatatt
    10201 cttgaagcat tgcattaata ccatgaacgc cagcatgttt accgacttgt aaccatctgg
    10261 tcctacctac aatctcagga ctgataggtt cgtaagtaag tggattgctc aaaactccat
    10321 gagtatgtat gccagattcg tgtccaaacg cattctcacc tataattgcc ttgttgggct
    10381 gaactggcat tccagctaat tttgaaacat atttcgatgt atcataaaga agtttagttt
    10441 tgatattagt ttcccaagac tgatcaaatt ttaaacattg taacgccatc actaattctt
    10501 ctaatgatgc attacctgca cgttcaccaa taccgtttat cgttacatgt gcacattgtg
    10561 cacctgcttg tattccagat atagcattag ccacagcaag accaaaatca ttgtgacaat
    10621 gcatactgat tggtaattta gttgcctcaa ttgcatcctt agttatttca gcaatatatt
    10681 gtggtgttga atatccgaca gtatcaggta tatctattct atccgctcca gcttttgtaa
    10741 ctgccgtaaa tatttttttt aagaaattac gatctgtacg tgttgcatcc tctgctgaaa
    10801 actcaacctg caatcccctt gattttgcat actcaactga ttctacagcc ttctcaagtg
    10861 cttcatcctg tgaaaggtgt aatttatgtt ctaggtgaat gtctgaagtt gcaataaatg
    10921 tgtgaacgta ttttaggcca caatcaacta ctgcgtcaat atctttttta tttgttctag
    10981 caagaccaca taattcagca gaaagatttg cttctgtaat catttttacc gcctgctgtt
    11041 caccttgtga gattacagga aatcctgtct caatagcatc cactcctaac tcgtctaatt
    11101 tttttgcgat gcttaactta ttttcaggtg ataatgcaac acctggtgat tgttcgccat
    11161 ccctcaatgt tgtatcaaaa atccggactt tcattttaca tcatctataa tgcagtaaca
    11221 ccagtcctag cagaatctaa aataccaaat ggctttaaca cttcctcgaa tgttttaatt
    11281 ttatcaggtg tagatgttaa ctcaactatc aatgaatctt tctgttcatt atgaactttt
    11341 gctccatatg tatctgctaa cttattgatc tctatactat ctgatgcctc acttaactta
    11401 attttaaata atgccaattc tttatacaca ctctcattct catctagtct tttgacctca
    11461 attgtatcaa tcattttatc aagttgttta acaatttgag caatttgttt ttcagtgcca
    11521 acagttgtta ttgtcatctt tgaaaattca ggattctcca ttacacctac tgatatacta
    11581 tctatattga aatttctaga tctaaacaag tgggtaacct tgaataatat tcctggtttg
    11641 ttttcaaccc gaattgataa tattgcccac ataacactca ctatgatggt aagatcatat
    11701 cctttaatga agttccaggt gcaacaaatg gtaaaacatc ttcttctgga tcaattggta
    11761 tatcaattac tgtgctaact tcactgttaa gtccaacctt gacagcttta ccaagctcgt
    11821 caagtgattg agcccttata ccttgtgcac catatgattc tgctaattta acataatctg
    11881 gacatttttt taaatcaatt ccaatcattc gtctttcata gaatgttctt tgccattgtg
    11941 caaccattcc taatgaaaaa ttattaatta agaatactat gacgggtata tcctctaata
    12001 ctgaagtcgc aagtgagttt tctgtcatgt taaaacttcc atctccggca atatctaata
    12061 ctggtacgtc tggccgtgca acttttgcac caatggcagc gggaaatccc catcccattg
    12121 ttccaagacc agtagagcta aagaatgtac ctggatgtat tgcatcaaag aataatgatg
    12181 cccacatttg atgctgacct acttcagtag taacaattga ttctttagga agcacttccc
    12241 gtaacttcct taaaatcttg gcagctgaca tttcacctgg atgtaatttt agattttcac
    12301 gccaataatc cttaacctca ttaacatgtt tcaaccattt atttccatca gatttcttta
    12361 atactttttt agcaagaatt ttgagcataa ctcttaatga tgccctaacg tcaccaacaa
    12421 ctgcaacttg agttgtctga ttctttccta tttctgcagg atccacatcc atatgaataa
    12481 tttttaaatt tttttcaaac tcttcaaatg ttccaacaga acgatctgaa aatcttgttc
    12541 ctatggctaa aacacaatct acttcagaca taatcttatt agcttctgca tgtccatgca
    12601 tgccaattgg accaagtgaa agtggatggt tctccggaaa agaacccttg cccttaaatg
    12661 ttgtaacaac tggaatcatt aacatttcag cgattgattg aagttctgca aatgcagaag
    12721 aaattattgt tccacctcca gccaaaatta ttggtttttc tgaagaaaga agtaaatcta
    12781 tagatctttc aatattagtt atatctacat cagtccatgg atgataacca ggaattctta
    12841 cttcgttagg aaaagtaatt tccgcctcat tttgttgaac atcttttggt atgtcaagta
    12901 atacaggtcc aggtctacca gtttctgcaa tgtaaaatcc tttttttaca acttcgggaa
    12961 tttcttgagg tgttcttggt tgaaatgcat attttacaca tggatttgac attccaatta
    13021 tatcactttc ttgaaatgca tcacgtccaa tcatgttcac tggtacttgt ccagtaacag
    13081 caatcattgg ggcagaatct gcttgcgccg ttgcaatacc agttaacaaa tttgttgccc
    13141 caggaccaga tgttgcaaaa caaacacctg gttttctact gacacggcca aatccatcag
    13201 ccatatgtgc agcagattgt tcatgtctta ctaagatatg acgtatatta cttttgtaaa
    13261 gctcatcata cattggaagg tttgcacctc ccggtaagcc aaagacttcc ttaacgcctt
    13321 ctttttctag cgctttcata agcgctttcc cgccattcat aacttccatt ttatcattca
    13381 tatcatacac cataatttta attgttaaaa aattggctgt atatttacag cttcaaatca
    13441 actagcaata gaatacttac aatgctagtt tgactaattt tgctcatata gcaaggtaaa
    13501 caacattaat tgaattaatt aatgtttctg gttgtgaatt taagacctta aatttctgtt
    13561 aacacttaaa ccgatgaaat agaaataaaa atggtttgtc tgacgaacaa gaaattcttg
    13621 aagtaattcg aacttttttc gaagccggta gaacaaagga tttctctata ctgagtaaaa
    13681 ttcaacttaa ttcacaaaaa ttttctagtt ttagtgatat tccaccatat ggattgaaag
    13741 attattcaga atcaatagct ctggaagaat taagattctc aagcattaca gattacaatt
    13801 atgaaataaa aaacgagaag accaatattt ttgatagtgt tggaatctcc acatttacat
    13861 tggaacaaaa gggtatgcta gtggatagtc aaacttacac cggtcagtat atgacaatta
    13921 atggtagagc tacatttgtt ttgattaaga aaccaacttg gaaaattgtg cattatcatc
    13981 tagataaaat ttctaattaa tttttatgta ttatttttta gtttttggtg actttggttt
    14041 aggctgacat aattttttgt ataattcgta ggcttcatca acatttgcgt ttccatgtat
    14101 aatattatga accgccctga tcattggtac tgggttttca gattgccaga tatttcgtcc
    14161 catatcaact cccacagcac ctcctttgat tgaattgtat gctaattcaa gtgcatctct
    14221 ttccggtatt tttttaccac ctgcaacaat aattggtact gggcaagaat ctactacacg
    14281 ctcaaaatta tcacaataat atgtcttaac aatatgtgca ccatgctcag ctgctattct
    14341 acatgcaaga gaaagataac gtgcatcttt ctgaccaatt tctttaccaa cagctgttac
    14401 tgctaagaca ggaagattat actcatttgc atcattaaca agtttactaa gatttacaat
    14461 tgtttgatgt tcatgttttg ctccaacaaa aattgacatt gccaatgctg atgcatttaa
    14521 tcgtatggca tccttgatac ttacagtaat ttgttcattg gataaatcct gaccaattat
    14581 actggcaccg ccagatactc ttagaactat tggtacagga tatgtcgggt taactgaggt
    14641 tctttgcact cctcttgtta gcatcaatga atcgcagtat ttgagtaatg gtcctattgt
    14701 ttttttagga atttctagct tttcagttgg accaagaaaa tatccatgat caacagccag
    14761 catcaaagcc ctattatcat taggtttgat tatatgagca agcctatttt ttaatcccca
    14821 atccatttgt atcgttttca aaagaaatca tatctttctt aagccttttc attcgcttat
    14881 gattatcttc atggcgtttt ctccactctt ggcatgttca aaggcttttt ggcattcatt
    14941 tagtttgtat ttatgagtga ttaattgtgt aacattgatt tgtttacttg atattttctc
    15001 aagtgcatct ctagtatcca tgtcagatgc tgcataagaa ttaacaattg taatactttt
    15061 tgaatacact ttactcatgt ctagctcaac agttgctcct ttacttggta caccaaacat
    15121 aacaatagta cctccttttc taacaaaagt cacagcatct tgcagtgctt tcaaattacc
    15181 agtagccacg ataacgacat caacaccaaa tggatgctga agatttgatt tcataatttc
    15241 tgaagcagtt ggatcatcag aatgaattgt tatagtttca agtttttgtg caaaatctaa
    15301 gcgataatca ttcaaatcta aacaaaacac ttctttcaaa ccataaaatt ttgctaataa
    15361 tacatgcatt attccagtgg gaccaacacc aaggatagca acactatcgt ttgttttatg
    15421 tgtaaattta ttccatgctc taatgcagca tgcaagtggt tcaatcattg cagcttcctc
    15481 aaaggaaaca ttgtcaggaa ttttcagcac acctccatgt tttacattcc atccaggtac
    15541 gacatattca tcagcaagtc cacatggttc aagatttgat tcataatatt ttttacacat
    15601 tgtttcatta ccatgattac attcatgaca gtcgtctgaa taacatgcaa catgatgatg
    15661 agtaaataca cgatcaccaa tactaaaatc tgaaatttct gaaccgacct cagttattgt
    15721 tcctgctggc tcatgtccaa gtttcattga tgcttgtcca tatttaccaa atactttttc
    15781 aacgtcagaa ccacaaattc cacaagcatg cattttgaca agtatatcat ccgaacctaa
    15841 agatggttta atcatatttt taatttctat attggagtca ctcttcacaa ttgctgcttt
    15901 catgaaattt atactcaata attatatgta aagtatggta ttaactgata atttatgcga
    15961 aaataaacaa ttttaattat agatgaatca taaattcaaa aatggctaaa attggtacat
    16021 ttattttatt gggattaatt ataggctcga ttagtttctc actttttcta tttttggaca
    16081 cacaaactat tactgtaatt tcagagtcag gtggtgcagt gattgctggt gatgttaaat
    16141 tctacgtaga acatattggg aatcatgaaa ttctcaaaaa aacagatgag tacagtgaag
    16201 cagaaaaaaa tctagttcaa aaaggcttgg atagaagtga aattcctgag ggagtttatt
    16261 tccaaattca gattaatgct cataacaccg gagatgaaac tgtaagagtg actggtggac
    16321 aattttatct ctatgataca gataatgaaa aatatgaagc tgtttttatt ggatatggtg
    16381 aagaagaact atcagtaata gatcttaaac ctaatgaaac aattgttgta acaacacaat
    16441 ttgatattcc ttacgatgaa gaaatgaaat atacaattgg tataattcca aataaatatg
    16501 gtctacagga tgcacaagaa agaatatttg tttgtgtgaa acaatgcgaa gtttgattat
    16561 tttttgagat cagataatag tccagataaa aaatctctat attcagtatc agagaaagta
    16621 actgattcta tatctgttgc atgatttatt gtttttaatg aatctaaatg atagcaattt
    16681 ttttcattat tttttttagt aaagtaataa ccagggcatg aacaaaaatc taaatcagga
    16741 tcaagccaat attctctatc cgaaccaaca acagtccaaa tttttctatt actaggttca
    16801 aagacatgaa gttttatgcg tttttcattt agaaccgaat tatatttttt agatgattta
    16861 ttcataagaa tttaataaaa aaggttgatt atatttttga tggtgaaaac tagaatcgtc
    16921 ccaaattatc ctgctttatt atcaactggt tctgaaaaaa atctgattat aactgattta
    16981 catttaggct tggaaggtaa tttgtcactg aataaagtat tcgtagaaaa aaatgtaaca
    17041 gtaaaaaaaa caataataga aattgaaaaa ttgcttaaaa taacaaaacc tgactcgtta
    17101 attcttcttg gtgacataaa atctggaatc aaatctataa caaaaactga atgggaatca
    17161 atcccaatgt tttttgataa actaaaaaat cgagtaaatc taatacttgt tccaggaaat
    17221 catgattcta atatagaaaa acttgtgcca aaggaagtta atatcgcaag cccgaagggg
    17281 atcataatag atgaaatact ttttacacat ggtcacacat tgcctacaga aaactacggt
    17341 aacgtaagca aaattgtaat gggtcattta cacccagttt ttttccaaaa agagtcaata
    17401 attaatggta aaagagtgtg gatatccata aaatgtagta aggatgagat tttcccatca
    17461 caaactggtg atgttgaatt gataattgtt ccagcattta acaaatactt ccatatgaca
    17521 aagaaaaaat tctataaaaa atcaatatca ccaataattg aaaaaattaa cactacaaaa
    17581 gccaaaatta taacacttga tggtacaatc attggtgatg aaaaattact tccaaatcta
    17641 atctaattgt aatcttcgta tggttctaat gctttttttg tgggtgtgtt attctgtaac
    17701 cattctaatg ttttcacaaa tgactcagcc agttcaatgg tagtcaaaac aggtatacca
    17761 agttctaatg attttcttcg tatttgatat tcatcataaa gcattccaac atatttttca
    17821 atagttgatg tacttgggat gtttataata aaatcaattt ttttctggta aagtagatca
    17881 gatatgtttg gttttctatc tggctcacta attttatgta caatttcaac attacctaat
    17941 ttttcattta aaaattcagc agtagtttca gttgcaagta tcttaaatcc aagtttttgt
    18001 aaaagtgcag atgtttgtaa taatttttct ctattcttaa caccaccaat agtaacaagt
    18061 gcagaaccgg atttaggaag attatatcct actgatgtca aaccttttga tagggcatcg
    18121 taaaaactat caccaaaaca tgcagcttcg ccggtggatt gcatctccac acccagaaca
    18181 atatctgcac catctaattg catgaaggaa aattgtggaa ctttaattcc atgaatagga
    18241 atttttttcc attcgtcata aggtatatgt ggtaattgtt ttcctaatac agcctttgat
    18301 gcaagagaaa ttagattcat tctaacaagt tttgatacaa atggcatgga acgtgatgca
    18361 cgaatattta gttctattac gtattcctga tcattattaa ttaaaaattg aagattgaaa
    18421 ggacctttaa ttttaaattc cctggcaatt cttactgtgg aatcagtaat attctcaatg
    18481 attctattac ttattctcca aggtggtata cacatcattg catcaccaga atgaacacct
    18541 gcactatcga tgtgctcaac tattgcacca attacaacat ttttaccgtc agaaacacca
    18601 tcaacatcaa cttccagtga atctagcatg aattttgtaa taactactgg gtaatcaggt
    18661 gatacaactg cagcgtttgt tacaaattcc tttaattcct cctgtgacca tacaactttc
    18721 attgcggctc cgcttagtac gtatgaaggt ctaaccaata caggataacc tacatctaat
    18781 gcaaagtttt ttgcatccgt aatattagaa aatgcctgcc aaagtggctg ctttatgtga
    18841 agattgtcca agatttcact gaactttgag cgattctcag cacgatctac attttctgca
    18901 ctagtaccaa tcaaattaat tccattttta gcaaggtttg gggtgaggtt gttagcagtt
    18961 tgaccaccaa cagatgttac aataccttta gggttttcaa attctgcaat atctaaaata
    19021 cgttctagtg ttaattcttc aaagtaaagt ctactgcata tatcataatc agttgataca
    19081 gtttcagggt tacagtttac aacactaaca ttcttttcac cattttcctg caaaccccaa
    19141 accatattga ccgtacccca atcaaattca acactgctcc caattctata tggtcctgca
    19201 cccaaaacaa ttacaccttt atctgattca tttatctcga tgtcattcgt ctcgccacca
    19261 taagtcagat aaagataatt tgtttttgca ggccattcag cggcaagtgt atcgatttgt
    19321 ttcacagagg ggattatatt gtaatcttta cgtatttttc taacttctgt actttgcata
    19381 tcttttgcac gtgcaatttg attatctgaa aatccaagct tttttgcttc ttgtaaaagt
    19441 ggtttttcca attcattttg ttttagtttt tcttctgttt ctacaatatt ttttattttt
    19501 tcaataaacc aaggatcaat tgatgataaa tcataaattt tttgaacagt tattcctttt
    19561 tttagagctg ctgcaacaaa atatagaata aaatcatcat gatgctgcaa atgatcttct
    19621 atatcttcta catcaaattt ttgaccattt tcacggttca atacaagacc atcttttcca
    19681 atatctaaca ttcgtattgc tttttgtagt gattcttcaa atgttcttcc aacagccatt
    19741 acttcaccaa ctgatttcat agttactcct agtttacgat taacaagttc aaatttttca
    19801 aaatcccatc ttgggtgttt acaaacaata taatctaatg atggttcaaa acatgcagta
    19861 gtagactttg taatacgatt cacaagttct ggaagtgaat aacccaatcc tatttttgca
    19921 gacatgtatg ctagcggata accagttgcc ttgctagcaa gagccgaaga acgtgaaaga
    19981 cgtggattga tctcaatagc aacatatctg tcagaatcag agtctaatgc atactgaata
    20041 ttgcattcac caacgattcc aacatgtttt gtagcacgta ttgcagcaga acgtaacgtg
    20101 tgatattcat gattattgat tgtttgcgat ggtgcaacaa caatattatc gcctgtgtgt
    20161 actttcattg aaagaacatt ttccatattg cacacaatta cattatttcc accataatcc
    20221 tgcattacct catattcaat ttgcttccaa tggccaatat actcttcaac taggatttgc
    20281 ccaacaagac ttgctgcaat tccacgttct gcaatctcat gcaattcaat ttcattatgt
    20341 gccacaccac caccttttcc tccaagagta taggctacac gaacaattac tggataacct
    20401 aattcttccg caactttttt ggcaccctca aaagtgtgaa ctgtcttact cttcaaaaca
    20461 ggaacaccac atttttgcat attatctttg aatttctgcc tatcttcagt agccataatt
    20521 cctgggattt gggttcccaa tactttgaca tcatattttt gtaaaatacc catttcgtct
    20581 aattttacac cacaattcaa tgcagtctgc ccgccatatg acaacatgat tgaatcaggt
    20641 cgttccgatt caattacaga ttcaacatat tgaggagtaa caggttgaag atagactttg
    20701 tctgcaaatc tagtgtcagt ttgaattgtt gcgatgtttg gattaattag aacacttttg
    20761 agcccatctt catgaattgc tttaagacat tgacttccag aataatcaaa ctctccggct
    20821 tcaccaattt ttattgcacc acttccaagt acaagaattt tctttagtga ttcatttttt
    20881 ggcaatttgt tatttctcca tcaatttttt tagttcatca aaaacatatt tacagtcaaa
    20941 aggaccaggt gcagcttcgg gatggaattg tacagcaatg caattttgtg ttttatgttt
    21001 tatgccttca actgtgttat catcagcgtt tgaaaaccat agtttaaaat cagattgttc
    21061 taaagattca ggtttgattc cataaccatg attctgactc gtaacataaa cctgattcgt
    21121 ttctaaatta atgcaaggtt tattctgacc acgatgccca tactttagtt tataagtttc
    21181 agtatttcct gcaatgccaa taatttgtgc acccaaacaa atacctaatg taggaatatt
    21241 attttcaatt aatttttttg cggtatcaat agtttcagga caattttgag gatcaccagg
    21301 accactactc aaaacaactc cctttggatt atatgacatt actttattga acggagtgtt
    21361 ccatggaaca actattgctt tgtatcctaa ttctcgaata tttctaacaa tggcattttt
    21421 tgctccagtg tcaataacaa ctacagattg attatcatca ccatagattt tttcctcttt
    21481 ggtagataca atatccataa atttttcaga attgtatttt gtaacgttag taagttcctt
    21541 tttgattttt tcaacatcaa tttgttcatc agatacaaca agagcggcca tcatcacacc
    21601 actacttgta atctttttgg tgattgctct agtatcgatt ccagatatgc ctggaacttt
    21661 ttcattatac atccactcat ctagagtcat tttcagattc caatgacttg cagtttgaga
    21721 caactcgtga actactagtc ctcgaatttg catcatatct gattcaaaga atttagaaat
    21781 tccatcctca tcgacttcgg ttaaatctgg aattccataa ttcccaacca gtggatatgt
    21841 cagtgtaaga atttggccat tgtatgaggg atcagttaga gcttctgtat aaccaaccat
    21901 acctgtgtta aagacaattt caccaaaaac tgtggtagaa aaaccaaatc ccattccatc
    21961 aagaatggtg ccatcagaaa ggatcagctt tccaaacttg tttccatgtt tggatttttt
    22021 tatggctatt ggaaccctcg ataactgctt taattgagaa atttaagttt ttgtaaaatt
    22081 tgtgctgata aaataagaaa ttactcttct ataatgcctt aaactgttca ctccatttat
    22141 aatatgccct gatcatttcc acggaaacac ttggctttcg tctttctaga atttctttaa
    22201 aatccttcaa cgtcaagtcc cgtggctgag tagggttttc acctgctact ggttccttaa
    22261 attgaggtga gcaaaataac tcattcacta ctttcatttg tgatgcctgg caaatatctt
    22321 taatatcact tgcactatac ccatcaaaaa ttgtagctag tgaacgagtg cgtactctac
    22381 tatccatttt taattgatgt gtgtaaagat caaaaagttt tgtacgtgca tcaattgtag
    22441 gtagtgaaac atagattctt ttttgaaatc tacgtaggaa tggattatca aggctccatg
    22501 gtttattggt tgcccctatc acgtataatt tgatactctt ccctttgtta ctgattccat
    22561 ctatttctgt caaaaattga ttcctggatc ttacttcacc gccaatttcg tttgtacgtt
    22621 cacctaataa tgaatcaagt tcgtcaatga ataatataac aggtttatcc tctctttcgg
    22681 cataactacg tgccatcctg aataattttg ccacattctt ttccgcctca ccaagccatt
    22741 tactcatcat tgacgatgca tcaacattta tgaaataacc atcaagttca tttgctgttg
    22801 ccgcagccag tatagtttta ccacaacctg gtggaccata tagtaatatt cctcttggcc
    22861 aacctaatgg aaatagatca ggtctttttg ttggccaaac aattgattct cgtaatgcat
    22921 tttttgcttc atcaactcca acaacatcac gccaattgat gtccggtttt tctttcatta
    22981 tcatttcttc aaaactatcc tgtcgtgctt ttccaagatt tgattgttgt tcttctggcg
    23041 ttgctttagg atcaactgct ggttcaaaat cattgccctg caaagattga agtgctttaa
    23101 tcctattttg atatgatttt aatctctcag catagatagt gtttagttta ctatctgggt
    23161 aaagcttcat caatttagat agtgattgta ttgccttttc ataatgagca atagccattc
    23221 cacgtgcacc ttgtgaatca tattttatag catctgaagc agatttgcta gcatttcttt
    23281 ctaattcttg tggtgctaaa ttcattcaaa ttaccttgtg aacatcagtg aagttttgct
    23341 aaggtac
//
LOCUS       DASCPJ010000085        14549 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001390111, whole genome shotgun sequence.
ACCESSION   DASCPJ010000085 DASCPJ010000000
VERSION     DASCPJ010000085.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001390111"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="QGF01_03915"
     CDS             <1..992
                     /locus_tag="QGF01_03915"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HJN20073.1"
                     /translation="KAKALDALRSLLPASTLTNVGITGNGRAFEYLLSILLSSNLAEE
                     RDLALAMKGQLDTTIKSFVGRANDKYGKVLQKYLHDIRKNSIKLGKKYAKGKPVQGNT
                     VKLVESDTESSALDSVISALIYEQSSGIQFLQIKKNIKKISKRQKAQIINDFAQIRKN
                     RRHRPPRAFEMTYYTFDLVTNYGMFRDFHRHRALTLERQLLSTDHGYHTPNEIIELGI
                     EKEFVDCMKRTKNVFQKIRVKMPEQAQYVVNFSYNYPYYMKLNLREATHLIELRTVPQ
                     GHIDYRKVAQKMYRSINQKHPALSKIIKFVDLNQYGLERFESEKRTEEKRKKI"
     gene            989..1387
                     /gene="msrB"
                     /locus_tag="QGF01_03920"
     CDS             989..1387
                     /gene="msrB"
                     /locus_tag="QGF01_03920"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006618845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HJN20074.1"
                     /translation="MTYKITKTDEGWKTQLTPEQFEVCRKKGTEQAFTGIYWNCKDNG
                     TYNCLCCGTKLFSSDTKFDSGTGWPSFFEPIDKKDIEYIEDTSFGMIRIEVNCKNCGA
                     HLGHVFDDGPQPTNLRYCINSVSLDLHKLD"
     gene            1413..2423
                     /locus_tag="QGF01_03925"
     CDS             1413..2423
                     /locus_tag="QGF01_03925"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HJN20075.1"
                     /translation="MRIILCGFGVVGQSLAKLLESRAEDLYARYGLKPRIIGVFDSKG
                     SAVDVSGLDVTRLIQIKKKYGSVKKYSNTKNNVSGIQMINELEAEVLIETTASNYKDA
                     EPGMSHIINAMKHGMHVISVNKGPLALAFPSLMELATYNHVIFRFSGTVGGGTPILDY
                     AKNSLKGERIISFDGILNGTTNYILTNMTNGMSFKDALNDAKKRGYVEADESLDLDGL
                     DAAAKLVILANWIMGMKVTMPEIKRIGIRKIDSDDIRKAAANNSAIKLIASCDNGLVV
                     KPKELPFDNPLCVSGTLNAISFTSEHSGTQTIIGRGAGGIETASSILRDLIDIRNEIV
                     KI"
     gene            2420..2896
                     /locus_tag="QGF01_03930"
     CDS             2420..2896
                     /locus_tag="QGF01_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HJN20076.1"
                     /translation="MKSNLISKSKTAEILEQINLQWNVKLSKQKNVRFHYITDNEIII
                     TGTDITAIMVGNDILPFLNETSILEKFPSVVVDMGAIQFVCKGANIMRPGIKEFSDFK
                     KGDIVCVIEESQKKILAVGIAEMSSDELKEAKKGEVIKNIHYISDNFWEIGKELSL"
     gene            complement(2886..3479)
                     /locus_tag="QGF01_03935"
     CDS             complement(2886..3479)
                     /locus_tag="QGF01_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HJN20077.1"
                     /translation="MPGATAVGITYNDGVVFASERRIAYGNFLVSKTTKKTFVITDKV
                     GATCAGLVADMQILSLQVAALAKIRKMEIKRDVPPNTVAKMMSNMMYERRFFPLLTQV
                     IVGGLVDKPTIYTLDPLGSVLPDEYAAVGTGAEMALGVLDPQYKKQMNEAEAIDLAVK
                     AVRSASLRDSASGDGIDVLIINKNGVKEVSEKILATS"
     gene            complement(3551..4912)
                     /locus_tag="QGF01_03940"
     CDS             complement(3551..4912)
                     /locus_tag="QGF01_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HJN20078.1"
                     /translation="MSIKKVLQYVDKNMNTLVNDLCILIQQPSVSAKNQGIKKCANLV
                     KNTLKKSGIKAEILSTKNYPPIVYGEIKSKNNPHKTLLFYNHYDVQPVEPIDLWDDDP
                     FSGKIDGNKIYGRGSSDDKGEMITRIKAVESFLKETGDVPCNVKFVIEGEEETGSAHI
                     EQYLKKYKKKFSCDGIIWEFGHVDNNDRPIIDLGMKGLLYVELSLRESNMDSHSSLAV
                     LIKNPAWRLIDALKTLRDPSGKILIKNWYNEVKPLSNEDIKILKSFPFNEVEFKKEFG
                     IKQFLGNKKGLVIKKSLAAEPTCNIAGIVSGYHAEGAKTVIPAEARVKIDFRLVPNMD
                     PKKQEIRLRKHLKSNGFGDIKIKVYHGEAAARTDSKHPFVSHVTSAASKSFGKYILSV
                     SSAGTGPMHSFTKILNAPAIAIGSTHVFSRIHSPNEYTRIDLLNKATKCMCIILENFS
                     KSR"
     gene            complement(4909..5769)
                     /locus_tag="QGF01_03945"
     CDS             complement(4909..5769)
                     /locus_tag="QGF01_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HJN20079.1"
                     /translation="MSIIITSKLVKKFIPVRKSSSRKGDNGKVLVLGGSYIYHGAPAL
                     SSLAALRTGADLVYTCVPKINVQSTRAVSPNLIVIPLADSKLTRGAVNKLLGQIPRDL
                     DSATIGMGLSIQDPEALKLLVKSLLDRDVRLSLDASALVNYILPFLSGKNVIVTPHPG
                     EFKRLFGEVPPESKKTRITMVEKFAKKHSVTIILKGSTDIISNGTTTYLNPKNIPAMT
                     VGGTGDILSGIIAGILAQNRDVLESAVTATYFNGLAGKMTQRKFGTHMTATDLLDVLP
                     NVMKSFDKIK"
     gene            complement(5779..6633)
                     /locus_tag="QGF01_03950"
     CDS             complement(5779..6633)
                     /locus_tag="QGF01_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HJN20080.1"
                     /translation="MGKIKQNNYVLFFYNDSKKWLIKISKNDQLHTHIGVIKHSDAIG
                     KEYGSRLVTNKDKYVYLYEPTIHDFVMKIQHGTQIVYPKDLGYIVARTGLTSGQKVVE
                     IGTGSGSLTSFLAGIVKSRGHVYTYDVEPKFMQIAEKNIKKAGMSKYVTMNKFDFKKA
                     KKVPHSEIDLVVVDLGDSWNVVPQARKMLKGSGGFIAICPTMNQLEKLVSVLIQNEFS
                     DIECTEQILRHIDAREGKTRHSFRGIGHTTYLAYARKSFFDIKPVKKSKLVKAKTVSK
                     KKVYSPKK"
     gene            6870..7034
                     /locus_tag="QGF01_03955"
     CDS             6870..7034
                     /locus_tag="QGF01_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20081.1"
                     /translation="MTEPPKDIEEYFENSKCKICGVVVQPHFQGNTKIPKEEHLTEKH
                     NFAKHQFGKT"
     gene            7199..7600
                     /locus_tag="QGF01_03960"
     CDS             7199..7600
                     /locus_tag="QGF01_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HJN20082.1"
                     /translation="MDKERFPDNHAIKKQKADLTEADLTEADLSGTDLRGANLIDADL
                     SNANLSGANLSNANLTRADLSGASLTRADLSGANLFRANLRRANLYHANLNDANLTEA
                     NLNNTDLTEANLSDADLSNANLSYAKGLDQH"
     gene            complement(7751..9631)
                     /gene="argS"
                     /locus_tag="QGF01_03965"
     CDS             complement(7751..9631)
                     /gene="argS"
                     /locus_tag="QGF01_03965"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HJN20083.1"
                     /translation="MAFLSILNQVKQHISKIIEEKGYDKVNFVVESSKSGFGDITCNI
                     AFLLAKNLKKTPQQIAEEFASAYDTGSADQLDRVEAHQSGYINFFLDTNSFNKSILSE
                     SQKKNYGSTDIGNNSKLIVEHTSVNPNKALHIGHLRNVAIGDALSRILSKVNYDVQVL
                     NYVDDSGLQVADIIVGFKFGGFSESPPPGEKFANYCGDTVYVKTTGKYENDKELESKR
                     GEILMELEDPESDTAKFGKIITRKVLTDQLDTVWKLGASYNCLNFESQILYSKLWEKI
                     FEQMKSENLVRFEEQGDNAGCWVLPIENEDDKILVRSNGVATYVAKDIPYAAWKLGVL
                     NDPFNYTVFSDEQIDDKLLWETTLEETQSEKKSFAANVVITVIDNRQTNLQKIITSLM
                     SKFKPGSSYVHLGYEAVTLSSETAKLLGNKTDGKDVQMSGRKGLYINADEMIQKLEKR
                     IFLESKERNDDLDDESLKKIAHNVAIGTIRYELIKPDLDKIITFDINTSLKLEGDTCS
                     YIQYSCVRAARILEKAGKQPGFDVQFDQLNNEYEINLIKQIAHFDVFVNDAAKNYSPK
                     VIARYCYDLAVTFSSFYEHVKVLKAETSELINARLCLVLSFKMTLEKALDLLGIIAPK
                     RM"
     gene            complement(9634..10329)
                     /locus_tag="QGF01_03970"
     CDS             complement(9634..10329)
                     /locus_tag="QGF01_03970"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HJN20084.1"
                     /translation="MRKKIIAVDIDGTITLNGMGAIHLGALSRLRALKEEGHSIIPVT
                     GRSSVEGYLLSIFGGLTHMAVGENGGCITFSDKIKHKLLGNKGECIHALATIQTKLDS
                     EIKEKPVFPRMTEVVLERTFDIEKARKIINDENLNVVLTDSGYAFHINSKGIDKGSGF
                     LEALKMLDADLNDAIVIGDSDTDIPLFKIVKNSIAVANSTENLKKIAKIVTTKKSGEG
                     VLEGLDMISFEDD"
     gene            10400..11026
                     /locus_tag="QGF01_03975"
     CDS             10400..11026
                     /locus_tag="QGF01_03975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJN20085.1"
                     /translation="MDETKKLFDKWAVTGRAEEMEKGHGVTVNKFLDSISFDKPFSFL
                     DVGCGNGWIVRKIAQLPNCKKAVGIDKSKKMISVAISKQIYKKEQYTCTNLESWNYTG
                     KFDVVFSMESLYYSVPMEPALAKVFKMLKTDGFFYCGTDFYSDNHLTKRWKKVMKVPM
                     DLRSKTEWKKMFNEADFKTTTRQVKDTKHRAKWKREFGTLFVIGQKTC"
     gene            complement(11030..13507)
                     /locus_tag="QGF01_03980"
     CDS             complement(11030..13507)
                     /locus_tag="QGF01_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJN20086.1"
                     /translation="MKFWLVLLLAVITLPAVFAEKGSFVDEVKFIQYLDENTALEEVR
                     YGNLDIYYSRISSDRIESQDNRDGIQVFASSGGSYSILVNPAISDKFNPFSITDVRFA
                     LNYLVDRNLIVNELLGGHGKPMISNYGMYAADYLSIIDEIESFHFEYNPSYANDLITD
                     ALENAGAEKIHDTWYHDGEQIEISFFIRSDDPIRKSIGEILSYELENIGFKVKKDFGD
                     LNKAFVVVYGSNPAMQKWHLYTEGWGSSGFSKYDSVGLAQMYSPWFSNMPGNNDPTYW
                     NYKNDYIDTLTQKIYIGDFTSAQERTSLIEEATKEGVNESVRIFLASKTDQFIANENV
                     DGVINALGAGITTRFTPINANSDTNSLVIGVKQIYQGAWNPIAGFSDTYSNQVWLNLY
                     DPGVFSHPFTGKIIPIRTGWEVENFGNDKKISVPEDAIIWDIDNQNWKKVGSDQYAIS
                     KITFDLILGDWHHNQKMDLNDILYSIYFLSEWGSAPYENDKTYDSEYSAQAIQNAKTL
                     IGIKPLDDNTIEVYVDYWHFDKAEIAGWAAPWSSMPWEIMASMESAVLDGNVSFSRSG
                     AVIKNVNWLSLIVPNDSQIIRQHLIELKESGHVPSSLQGSEHDWEYFEGRYDAAINWI
                     DQNNHAVISNGPFYLDNYSPESRTITINSFDSHEYPFESGKWEKFEQVKFPKITNVKI
                     SDVVNSGESVSIYVETSDSSQVHYFILDPRGEINISGTSPVDNNSSKIVLSKSETLEL
                     DMGTNTIKIFASSDEVLRPDDYSTAFLVVDGETSFPTVPISEAESRSEGTSYTGIVLA
                     IIGAVIVGIIVYIRRKRKAKLSNVKTS"
     gene            13577..14035
                     /locus_tag="QGF01_03985"
     CDS             13577..14035
                     /locus_tag="QGF01_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20087.1"
                     /translation="MESKLRSALFDIQEGVPIDQVANNIHWKDFEGLVAEILVSKHFE
                     VLRNFRMKKPTMEIDVVGKHLDVTMLIDCKHWKRMSYSNLKIIVNKQIKRAEQYIQNK
                     KNMTVVPIIVTLYKEEASFVNGTPIIPIFQFSSFIDEFYGNLEYMNTIEK"
     gene            complement(14022..>14549)
                     /locus_tag="QGF01_03990"
     CDS             complement(14022..>14549)
                     /locus_tag="QGF01_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HJN20088.1"
                     /translation="EILVAKTSFPLRYGYANSTSMNIETGDLAGGGIGMLCLAFEQKK
                     YFLCWADANNMENGVREKIVAHLQNNGYNLVEMFTSDTHFTTMGVRNRNGYYQLGIVT
                     KPEKLADWCLSIAKKAEKNIASGQFEILENKAKVRVMGSGIFEHLSVALDNSLKMTQG
                     FMIGCVGIFLLSIFL"
BASE COUNT         4781 a         2527 c         2385 g         4856 t
ORIGIN      
        1 caaaagcaaa agcacttgat gcattaagaa gtttattacc agcatcaact ttgaccaatg
       61 ttggtatcac gggaaacggt cgtgcgtttg aatatctttt atcaatattg cttagttcaa
      121 atcttgcgga agaaagagat ttggctttgg caatgaaggg tcaactggat acaactatca
      181 aatcatttgt tgggcgtgcc aatgacaaat atggtaaagt tttacagaaa taccttcatg
      241 atatcagaaa aaactcgata aaactaggga aaaaatatgc aaagggaaaa cctgttcagg
      301 gaaatactgt taaactagtg gaatctgata ctgaatcaag cgcgttagat agtgttattt
      361 ctgcattaat ttatgagcaa tcttctggca tccaattctt acagattaaa aaaaatatta
      421 aaaaaattag caaaagacag aaagcacaaa tcataaatga ttttgcacaa atcagaaaaa
      481 ataggaggca tagaccacct agagcatttg agatgactta ctatacattt gacttggtta
      541 ccaattatgg aatgttcaga gattttcata gacatagagc attaacactt gaaagacaat
      601 tactatctac tgaccatggt tatcatacac caaatgaaat tatcgagttg ggaattgaaa
      661 aagaatttgt tgattgtatg aaaagaacaa aaaatgtttt ccaaaaaatt agagtgaaaa
      721 tgcctgagca agcacaatat gttgttaatt tttcatataa ttatccatat tatatgaaac
      781 taaatttgag agaagctaca catttaattg aattacggac agttccacaa ggacatattg
      841 attatagaaa ggttgcacaa aaaatgtata gatccataaa tcaaaaacat ccagcactct
      901 ctaaaattat aaaatttgtt gatttaaatc aatatggact agaacggttt gaatcagaaa
      961 aacgtacaga agagaaacga aaaaaaatat gacatataaa ataacaaaaa cagatgaagg
     1021 ttggaaaaca caattaacac ctgaacagtt tgaggtctgt aggaaaaaag gaacagaaca
     1081 agcgttcacg gggatttact ggaactgtaa agataatggg acttacaatt gtttatgctg
     1141 tggaacaaaa cttttttcat ctgatacgaa atttgattct ggtactggat ggccgagttt
     1201 ttttgagccc atcgataaga aggatataga atatatagaa gatacgagtt tcggcatgat
     1261 tagaattgag gttaactgca agaattgtgg tgctcattta ggacatgtat ttgatgatgg
     1321 gccacaacca actaatctta gatattgtat aaactctgtt tcattagatt tacataagtt
     1381 agactaaata atccatagga gattatgata ttatgagaat aatactatgt ggttttggtg
     1441 ttgttggcca aagtttagct aaacttctgg aatcacgtgc agaagacttg tatgcaagat
     1501 atggattgaa gcctagaatt ataggagttt ttgatagcaa gggcagtgca gttgatgtgt
     1561 ctggccttga tgttactaga ttgattcaaa ttaaaaaaaa atatggttca gtgaaaaaat
     1621 attctaatac aaaaaataat gtttccggaa ttcaaatgat aaatgagttg gaggctgaag
     1681 tcctgattga aacaactgct agtaactata aggatgcaga acctggcatg tcacatatta
     1741 ttaatgccat gaaacatgga atgcatgtaa tttcagtaaa caagggtcct cttgcactgg
     1801 catttccttc attgatggaa ctggctactt ataaccatgt tatttttcgt ttcagtggaa
     1861 cagttggtgg cggtacacca attttagact atgcaaaaaa tagtttgaag ggtgaacgga
     1921 ttatttcatt tgatggtata ctaaatggaa cgactaacta tattctaact aatatgacaa
     1981 atggaatgtc attcaaggat gcattgaatg atgccaaaaa aagaggttat gttgaggctg
     2041 atgaatcctt agatcttgat gggcttgatg ctgccgctaa actcgtgatt ttagcaaatt
     2101 ggatcatggg tatgaaagta actatgcctg aaataaaacg tataggtata cgaaaaattg
     2161 atagtgatga tataagaaag gctgcagcca ataattctgc aataaaatta attgcatctt
     2221 gtgataatgg attggtagtt aagccaaaag aacttccatt tgataaccca ttatgtgtta
     2281 gtggaacact aaatgcaata tcttttactt ctgaacattc aggaactcaa accatcatag
     2341 gtagaggtgc aggaggtatt gaaactgcaa gctcaatctt gcgtgattta attgatataa
     2401 gaaatgagat tgtaaagatt tgaaatcaaa tctaatctca aaaagtaaaa ctgctgaaat
     2461 acttgagcag atcaatttgc aatggaatgt aaaattatcc aaacaaaaga atgtaagatt
     2521 tcactatata acagataacg aaataatcat tactggtact gatatcactg ccataatggt
     2581 tgggaatgat attttaccat ttttgaatga gacatctatt ttggaaaaat ttcctagtgt
     2641 agtggttgat atgggtgcaa ttcaatttgt atgtaaggga gccaatataa tgaggccagg
     2701 tatcaaagaa ttttcagatt ttaagaaggg agatatagta tgtgtgatag aagaatcaca
     2761 aaaaaaaatt ttagctgtag gtattgcaga aatgtcaagt gatgaattaa aagaagctaa
     2821 aaaaggtgaa gtgataaaaa atatacatta tatttctgat aatttttggg agattggaaa
     2881 agaacttagc ttgtagcaag aattttttct gatacttctt ttacaccgtt tttattgatt
     2941 attagaacat caataccatc accactcgct gaatctctta gacttgcaga gcgaacagcc
     3001 tttacggcaa gatcgattgc ttctgcttca ttcatctgtt ttttgtattg tggatccaat
     3061 acacctagtg ccatttcagc acctgttcca actgccgcat actcatcagg taatacagag
     3121 cccaaaggat ccagagtgta tatggttggc ttgtcaacca atcctcctac aattacttgt
     3181 gttaacaatg gaaaaaatct acgttcgtac atcatgttag acatcatttt tgctactgta
     3241 tttggtggaa catctcgttt gatctccatt ttcctaattt tagctagtgc agcaacttgc
     3301 aatgaaagaa tctgcatatc tgccactaat ccagcacaag tggcacccac cttatcagtt
     3361 attacaaaag tttttttcgt tgttttactg acaagaaaat ttccataagc aattctacgc
     3421 tcacttgcaa ataccactcc atcattatac gtgataccca cagcggtagc acctggcata
     3481 tacatcgcag tcataaatca aggagtttta gagaagaata aaagtcatta cgagaaggtt
     3541 ttttaaaacc tcatctagac ttggaaaagt tttctagtat aatacacata cattttgtag
     3601 ccttgtttaa tagatctata cgtgtatatt cattgggaga atgaatacgt gagaaaacat
     3661 gtgtagaccc aatagcgata gcaggagcat tcaatatctt agtaaatgaa tgcataggac
     3721 cagttcctgc actcgaaaca ctcagtatgt attttccgaa tgacttactg gctgcagatg
     3781 taacatgaga aacaaacgga tgttttgaat cagttcttgc agccgcttca ccgtgatata
     3841 ctttaatttt gatatctcca aaaccgttag attttaggtg ttttcttaat ctaatttctt
     3901 gtttttttgg atccatattt ggtacaagtc taaaatcaat ttttacacgt gcctccgctg
     3961 gaattacagt ttttgcaccc tcagcgtgat aaccagaaac aataccagct atgttacaag
     4021 ttggctctgc tgcaagtgat ttttttatca cgagtccctt cttatttcct aaaaattgtt
     4081 taattccaaa ttcctttttg aattcaacct cattaaatgg aaaagatttc aagattttga
     4141 tatcttcgtt acttaatggc tttacttcat tataccagtt ttttatcaaa attttgccag
     4201 aaggatcacg aagtgttttc aatgcatcaa ttaaacgcca agcaggattc ttaatcaaaa
     4261 ctgccaaact tgaatgagaa tccatgttag attctcgtaa cgataactca acatacaaca
     4321 aacccttcat cccaagatca atgattggtc tgtcgttatt atcaacatga ccgaattccc
     4381 aaattattcc atcacatgaa aattttttct tatatttttt tagatattgc tctatatgtg
     4441 cacttcccgt ttcttcttca ccctctataa caaattttac gttacaagga acatctccag
     4501 tttcttttag gaatgattca acagccttta tgcgtgtaat catttcacct ttatcgtcag
     4561 atgagcctct tccatagatt ttattcccat ctatctttcc actaaatgga tcatcatccc
     4621 aaagatcaat tggttctact ggctgaacat cataatggtt gtaaaataac aaagttttgt
     4681 gtggattatt ctttgatttt atctcaccat aaacaatagg tggatagttt tttgtactca
     4741 aaatttcagc ttttatgcct gattttttta atgtgttttt tacaaggttt gcacattttt
     4801 tgattccctg gtttttagct gaaacacttg gttgttgtat taaaatacag agatcattga
     4861 caagtgtatt catatttttg tcaacatatt ggagaacttt ttttatgctc attttatttt
     4921 atcaaatgat ttcatgacat tgggtaaaac atcaagtaaa tctgttgcag tcatatgtgt
     4981 cccaaatttc ctttgagtca tcttccctgc aagtccgtta aagtatgtgg cagtaactgc
     5041 agattcaagt acatctctat tttgtgcaag aattccggct atgatgcctg aaagaatatc
     5101 acctgttcca ccaaccgtca ttgcaggaat attttttggg ttaagatatg tggtagtgcc
     5161 gttagaaatt atatcagttg agccctttaa aataattgta actgaatgtt tctttgcaaa
     5221 tttttcaacc attgtgatac gagtcttttt tgattctggg ggaacctcgc caaacagcct
     5281 tttaaactcg cctgggtgtg gagtgacaat tacatttttc ccagataaaa atggtaaaat
     5341 gtaatttacc aatgcagatg cgtcaagtga aagtctaaca tctctatcaa gtaacgattt
     5401 aaccaaaagc ttcaatgctt caggatcttg tattgaaaga cccataccta tagtggcaga
     5461 gtctaaatct ctagggattt gccccagtag tttattcact gctccacgag ttagttttga
     5521 gtcagctagg ggaattacta taagattggg tgaaactgca cgagtggatt gaacgttaat
     5581 ttttgggaca catgtgtata ctagatcagc gcctgtcctc aatgcagcaa gagatgataa
     5641 agctggtgca ccatgataga tgtagctacc accgagaacc aataccttgc cgttgtctcc
     5701 cttcctagag gatgattttc gtacgggtat gaattttttt actagctttg atgtaatgat
     5761 tatagacaac gcatctcctt atttttttgg tgaataaact ttctttttac ttacagtttt
     5821 tgccttaact agttttgatt tttttactgg ctttatatca aaaaatgact ttcttgcata
     5881 agcaagatat gtcgtgtgac ctattccacg aaatgaatgt cttgtttttc cctcacgtgc
     5941 gtcaatatga cgaagaattt gttcagtaca ctcaatatca gaaaattcat tttgaattaa
     6001 aacagatact agtttttcaa gttgattcat tgtagggcaa attgcaataa aaccaccact
     6061 accctttaac atctttcgtg cttgtggaac tacattccaa gaatctccaa gatcaactac
     6121 aactagatca atttctgaat gtggtacctt ttttgctttc ttaaaatcaa atttattcat
     6181 tgtaacatat tttgacattc ctgctttttt gatatttttt tcagcaattt gcataaattt
     6241 tggttcaaca tcatatgtgt atacatgacc tcgtgacttt actatcccag ccaaaaatga
     6301 tgtcaatgaa ccactaccgg ttccaatctc aaccactttt tggccgctag taagaccagt
     6361 tctagcaact atgtaaccca aatcctttgg ataaactatc tgagtaccat gttgtatctt
     6421 cattacaaaa tcgtgaatag ttggctcata aagatagacg tatttgtcct tatttgtcac
     6481 aagtctggaa ccatactcct tgccaatcgc gtcactatgt tttatcacac cgatatgtgt
     6541 atgaagttga tcattttttg aaattttgat cagccatttt tttgaatcgt tgtaaaagaa
     6601 caaaacatag ttgttctgct ttatttttcc catgaaaata gatactcaat ttgattatat
     6661 gtaataatcg actgtcaaat ttagttaatt agattctttt tcgtgcctaa acatcgaatt
     6721 ttttttcatg taagtagatt taaaatgaac gataagggaa aatgaataga aatggctaac
     6781 acacacagac attattgtaa aaaatgtggg ttgaagcgta ccgtaaagat gaacagtaaa
     6841 cttaccaaat gcacatgtga gcaattgaaa tgactgaacc ccctaaagat attgaagagt
     6901 attttgaaaa ctcaaaatgt aaaatatgtg gagttgtagt ccaaccacat tttcagggaa
     6961 ataccaaaat cccaaaagaa gaacatttaa ctgagaagca taattttgct aaacaccaat
     7021 tcggtaaaac ctagtatatt ggacaagata aatcccattc gtacatatta cattgccaca
     7081 attgtactag ttgttgcaac aatagcgttt tttattctag atttttagag acctaaacct
     7141 catactatca agcagagaac tggaattctt ttctataact tcaccattct aattttatat
     7201 ggacaaggaa agatttcctg acaatcatgc aataaagaag caaaaggcgg atttgactga
     7261 agcagatttg actgaggcgg atttgtctgg tactgattta cgtggtgcga atttgattga
     7321 tgcggatttg tctaatgcaa atttgtctgg tgcaaattta tctaatgcaa atttaactcg
     7381 tgcagattta tctggtgcaa gtttgactcg tgcagatttg tctggtgcaa atttgtttcg
     7441 tgcaaattta cgtcgtgcga acttgtatca tgcaaatttg aatgatgcaa atttaactga
     7501 agccaatttg aataatacgg atttgactga ggcaaattta tctgatgcgg atttgtctaa
     7561 tgcaaattta tcttatgcaa aggggttaga ccaacattaa tctctatgag attgatgcaa
     7621 ttaacttacc tgtttatatc agctgtaact cagtaaattg gaaaacccaa gttattgtgg
     7681 ttaatattaa tcataataag gacacttggg ttttccattt aatcatctat taaaaaaagt
     7741 aataatacat ctacatacgc ttaggcgcta taataccaag taaatcgagt gccttttcca
     7801 aagtcatctt gaatgataat acgagacata atcttgcgtt aatcaattca gatgtttctg
     7861 ctttcaagac tttgacatgt tcataaaatg aggaaaatgt gacagctaaa tcataacagt
     7921 atcttgcaat aacttttggg gaataatttt ttgcagcatc attcacaaaa acatcaaagt
     7981 gagcaatttg tttgataaga ttgatttcat attcattatt caattggtca aactgtacat
     8041 caaatccagg ttgtttacca gccttttcta atattcttgc agctctgaca catgaatatt
     8101 gaatgtaact acaagtgtct ccttcaagtt ttaatgacgt attgatatcg aaggtaataa
     8161 ttttatcaag atcaggtttg atcaattcat atcttatagt tccaattgca acattatggg
     8221 ctattttctt taaggattca tcatcaagat catcattcct ttcttttgat tcaagaaaaa
     8281 tacgtttttc gagtttttga atcatttcat ctgcgttgat gtaaagacct tttctgcctg
     8341 acatctgcac atccttacca tctgttttat tgcctagaag ttttgcagtt tcagagctta
     8401 gagtaactgc ttcatagcca agatgaacat aagaacttcc aggcttaaat tttgacatta
     8461 ggctggttat tattttttgc aggttagttt gcctattatc aataactgtt ataaccacat
     8521 ttgctgcaaa agattttttt tctgattgtg tttcttcaag tgttgtttcc cacaataact
     8581 tatcatctat ttgttcatca gaaaaaacag tgtagttaaa tggatcattc agtacaccaa
     8641 gtttccaagc tgcatatggt atatcctttg caacatatgt agcaactcca ttacttctga
     8701 caaggatttt atcatcttca ttctctattg gcaaaaccca acaaccagca ttatcacctt
     8761 gctcttcaaa tcgtacaagg ttttcagatt tcatttgttc gaatattttt tcccagagct
     8821 ttgaatacaa gatttgggat tcaaaattta agcagttgta tgatgcgcca agcttccaaa
     8881 ctgtatctaa ttgatcagtt aagacttttc tagttattat ttttccaaat tttgcagtat
     8941 cagattcagg atcttccaat tccattaaaa tttcacctct tttagattcc aattctttat
     9001 cattttcata ttttccagta gtcttcacgt aaactgtatc accacaataa tttgcaaatt
     9061 tttctccagg aggaggtgat tctgaaaagc ctccaaactt aaacccaaca attatgtctg
     9121 ctacctgaag gccagaatca tcaacataat ttaaaacctg aacatcataa tttacctttg
     9181 aaagaattct ggaaagtgca tcaccaattg ctacgttcct tagatgccca atatgtaatg
     9241 ccttatttgg attaactgag gtgtgttcta ctatcagctt agaattattg ccgatatcag
     9301 tgctaccata atttttcttc tgagattctg acaaaataga tttattgaat gagttcgtgt
     9361 caagaaaaaa attgatgtag ccactttgat gtgcttcaac cctatctaat tgatcagctg
     9421 aaccagtatc ataagcagat gcaaactctt ctgcaatttg ctgtggtgtt tttttgagat
     9481 tttttgctag taaaaatgca atattacaag taatatcacc aaatccactt ttagatgact
     9541 cgacaacaaa attcacctta tcatatcctt tttcttcaat aatttttgaa atatgctgtt
     9601 ttacttggtt tagtatacta agaaatgcca ttatcaatca tcttcgaatg atatcatatc
     9661 taacccttca aggacacctt ctcctgattt tttggttgta acaattttag ctattttttt
     9721 taggttttca gtagaatttg caacagcaat gctatttttc actattttga ataaaggaat
     9781 atcagtatca ctatcaccta tgacaattgc atcattcaaa tctgcgtcca acattttgag
     9841 tgcttctaaa aatccagagc ctttatcgat ccctttggaa ttaatatgaa aagcataacc
     9901 actatcagtc aaaacaacat taagattttc atcatttatt atttttcgtg ctttttctat
     9961 gtcaaatgtt cgttctaata caacttctgt catcctaggg aaaacaggtt tttcttttat
    10021 ttcactatct aattttgtct gaattgttgc aagggcatga atgcattcac ccttatttcc
    10081 aagtaactta tgtttaattt tatcactgaa ggtaatacag ccaccatttt ctccaacagc
    10141 catatgtgtt aacccaccaa atattgataa caaataaccc tctacagaag atcttcccgt
    10201 tacagggatt atagaatgac cttcttcctt caatgctctt agtctagata atgcaccaag
    10261 atgaattgca cccataccgt tcaaggttat tgtcccgtca atatcaactg caattatctt
    10321 ttttctcact atatttctca agatattcat caataataac tgaaacgttg aataatgaat
    10381 taaagataaa agtgacatga tggatgaaac taaaaaatta tttgacaaat gggccgttac
    10441 gggaagagct gaggagatgg aaaaaggaca tggtgttaca gttaacaagt ttttagatag
    10501 tatttcattt gacaaaccat tctcattttt ggacgttgga tgtgggaatg ggtggattgt
    10561 tagaaaaatt gcacaattac ctaactgtaa aaaggcagtt ggaatagaca aaagcaaaaa
    10621 aatgattagt gttgcaattt caaaacaaat ctacaaaaag gaacaataca cgtgtactaa
    10681 ccttgaatcc tggaactata ccggaaagtt tgacgtagtt ttttccatgg aatccttgta
    10741 ctactcagta ccgatggagc cagcactagc taaggttttc aagatgctca aaactgacgg
    10801 tttcttttac tgtggaaccg acttttacag tgacaaccat ctcaccaaga gatggaaaaa
    10861 agttatgaaa gtaccaatgg acttgcgttc taaaactgaa tggaaaaaga tgttcaacga
    10921 agctgatttt aagaccacta caagacaagt taaagacaca aaacatagag cgaagtggaa
    10981 aagggagttt ggcacactgt ttgtgattgg ccagaaaact tgctaggtct taagaagttt
    11041 taacattaga tagtttcgcc tttctttttc ttcgtatgta cacaattatt ccaactatca
    11101 ctgcaccaat tattgctaaa acaatgccag tgtatgatgt accctcagat ctagattctg
    11161 cctcagaaat aggaaccgtt ggaaaactag tttcaccatc aactacaaga aatgctgtac
    11221 tataatcatc tggtcttaat acctcatcag acgaagcaaa aattttgatg gtgttagttc
    11281 ccatatcaag ttctaatgtt tcagattttg atagtacaat tttggatgaa ttattatcaa
    11341 ctggactagt tcctgatata ttgatctcac ctcgaggatc caaaataaag taatggactt
    11401 gtgaagagtc tgatgtctca acataaatcg atactgattc acctgaattt acaacatctg
    11461 aaattttcac attggtaatt tttggaaatt taacctgttc aaatttttcc cacttacctg
    11521 attcaaaagg atattcgtga gaatcaaatg aattaatagt gattgtcctt gattctggag
    11581 aataattatc aagataaaat ggaccattgc tgataacggc atgattgttt tggtctatcc
    11641 aattgattgc agcatcatac cttccttcaa aatattccca atcatgttca gaaccttgta
    11701 atgatgatgg aacatggcct gactccttca attcaattag atgctgtcgt attatttgag
    11761 aatcgtttgg aactataagc gaaagccagt ttacattttt gataactgcc cctgaacgtg
    11821 aaaaagatac attcccatca agaactgcac tttccatgga tgccataatc tcccaaggca
    11881 tactactcca aggggctgcc catccagcaa tttcagcttt atcaaaatgc caataatcaa
    11941 cataaacctc aattgtgtta tcatctaatg gttttattcc gattaatgtt ttggcgtttt
    12001 gtattgcttg tgcagagtat tcagaatcat atgttttatc attttcatat ggtgcagaac
    12061 cccattctga tagaaaatat atcgaataca agatatcatt cagatccatc ttctgattgt
    12121 gatgccaatc accaagaatc aaatcaaatg taattttact tattgcatat tgatcacttc
    12181 ctactttttt ccagttttga ttgtctatat cccagatgat tgcgtcttca ggtactgata
    12241 tttttttatc attgccaaaa ttttcaacct cccatcctgt acgaattgga atgatctttc
    12301 cagtaaatgg atgactgaaa actcccgggt catatagatt cagccatact tgattactgt
    12361 atgtatcact aaaaccagca attggattcc atgcgccttg ataaatttgc ttaactccta
    12421 taaccaatga attggtgtca gagtttgcat ttattggtgt aaatcttgtt gtaattcctg
    12481 cacctaatgc attgataaca ccatcaacat tttcatttgc aataaattga tctgttttac
    12541 tagcaagaaa tattcttaca gattcattaa caccttcttt tgttgcttct tctatcaaag
    12601 atgttctttc ttgtgcagat gtaaaatcac cgatatagat tttttgtgtt aacgtatcaa
    12661 tatagtcatt tttgtaattc cagtaagtag gatcattatt acctggcatg ttagaaaacc
    12721 atggagaata catttgtgcg agtccaactg agtcatattt tgaaaatcca gagctgcccc
    12781 aaccttcagt ataaagatgc catttttgca tggcaggatt tgaaccgtac actacaacaa
    12841 atgccttatt cagatcacca aaatctttct tcactttaaa cccaatattt tctagttcat
    12901 atgataaaat ctcaccaata gatttcctga taggatcatc actacgtata aaaaatgata
    12961 tttcaatttg ttccccatca tgataccatg tatcatggat tttttcagca ccagcatttt
    13021 caagtgcgtc agtaattaga tcatttgcat aagatggatt gtattcaaaa tgaaatgatt
    13081 caatctcatc aataatagaa agataatcag cagcatacat tccataatta gaaatcatag
    13141 gtttaccatg acctcctaat agttcattaa caatgagatt tctatccact aaataattta
    13201 atgcaaatct tacatcagtg attgaaaacg gattaaattt gtcagaaatg gctggattaa
    13261 caagaatact gtatgatccg ccactagatg caaaaacctg tattccgtct ctattatctt
    13321 gactttcaat tctatctgag gaaattctag aataataaat atccaaatta ccgtaacgaa
    13381 cttcttctaa tgctgtattt tcatctaaat attgaataaa tttaacttcg tccacaaaac
    13441 ttcccttttc agcaaatact gccggtaatg tgatgactgc tagtaaaaga actagccaaa
    13501 atttcatggt atttgtcagc tatcaacata atttaaggtt cattaagtat taacaaatta
    13561 ttcacatagt gattgtttgg aaagcaaact gagatctgca ctatttgata tacaagaagg
    13621 tgttccgatt gatcaagttg caaataatat tcattggaaa gattttgagg gattagtggc
    13681 tgaaatactt gtatctaagc actttgaagt actgcgtaat tttaggatga aaaaaccaac
    13741 tatggaaatt gatgttgtag gaaagcatct agatgttaca atgttaatag attgtaagca
    13801 ttggaaaaga atgagttatt caaatcttaa aattattgtt aataaacaaa tcaaaagagc
    13861 agaacaatac attcaaaata aaaaaaatat gactgtcgtt ccaataattg tgactttata
    13921 caaagaagaa gccagtttcg ttaatggtac accaatcata cccatattcc aattttcttc
    13981 gtttatcgat gagttttatg gtaatttaga atatatgaac actatagaaa aatagacaac
    14041 aaaaatatcc caacacatcc tatcatgaat ccctgtgtca tttttaatga attgtcaagt
    14101 gccacagaaa gatgttcaaa gattcctgac cccatcactc ttacttttgc tttattttct
    14161 aaaatttcaa actgacctga agctatattt ttttccgctt tctttgcaat agataaacac
    14221 caatcagcta acttttcagg tttggtgaca attccaagtt gatagtaacc atttctgttt
    14281 ctcactccca ttgttgtaaa atgggtatca gaggtaaaca tttcaaccaa gttataacca
    14341 ttattttgta gatgtgctac aattttttca cgtacaccat tttccatatt attagcatca
    14401 gcccaacaca aaaagtattt tttctgctca aaagcaagac acagcattcc tataccacca
    14461 ccagcaagat caccagtttc aatattcata gatgttgaat ttgcatatcc atatcgtaat
    14521 ggaaaacttg tttttgcaac aagaatctc
//
LOCUS       DASCPJ010000086        14155 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001437194, whole genome shotgun sequence.
ACCESSION   DASCPJ010000086 DASCPJ010000000
VERSION     DASCPJ010000086.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 14155)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14155)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14155
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001437194"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="QGF01_03995"
     CDS             complement(<1..1348)
                     /locus_tag="QGF01_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HJN20089.1"
                     /translation="MSDSDDIQNIHKRYSFTLINPASFYVSLIFSIVTAAIISTLVIL
                     NYLQSGEILFTIPIVIAILLVTQYTDSRFTKHKEYSKSLHMSLFGNVLWLITVVGGIV
                     GAFIVSKELSLFYVAVGMYIFSSFRIGIMTTTLGISLKKSCVLCFIQPLAMFFVMVPM
                     DMWPILYDVQSLVFGIIFLAVACFWSYLTNRSGLPVIKSTHKLLQAYLQSVSHNDPRD
                     MESIILETSKSSNITTSQIRFYTNDEKNDFRMVLPDIHPGPFNPVGGSDIPNQIYKTM
                     NSSAMVMHSVSDHSLNLPSQQDVQTYLDGLSQSSVSTKGLMCTEPVTAQMNKARVVGI
                     RLDKSAVLLLSLSPHGMEDVPLYIKTEIEQIAKNRNFESVLVVDSHNAMGGKISQEDS
                     QDLITAAKNALEILVAKTSFPLRYGYANSTSMNIETGDLAGGGIGMLCLAFEQKKYF"
     gene            complement(1350..1520)
                     /locus_tag="QGF01_04000"
     CDS             complement(1350..1520)
                     /locus_tag="QGF01_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HJN20090.1"
                     /translation="MADKKKNAPLPASSAGLLRFFEDETRGVRIDPKIIISIPISLIA
                     VSWALELFLVIK"
     gene            complement(1560..1883)
                     /locus_tag="QGF01_04005"
     CDS             complement(1560..1883)
                     /locus_tag="QGF01_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HJN20091.1"
                     /translation="MSKDLTRKAVEMLLNGATLLAEPCPYCKGVRVIKDGNALCIDCG
                     KEPKQEAENIPNKHESTSTSKIMQNKLEKLSKQLEKEEDPVKQQELIKTIDSLISVID
                     KLNKK"
     gene            1989..2297
                     /gene="yciH"
                     /locus_tag="QGF01_04010"
     CDS             1989..2297
                     /gene="yciH"
                     /locus_tag="QGF01_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HJN20092.1"
                     /translation="MAVICSTCGLPEDLCACGDLDKDSNHIIIRLETRRFKKKGTMIE
                     GLDPKINNLESVGKDLKKKYACGGTAKNGYIFLQGDHRDTIKDTLIKLGFAAETIELH
                     "
     gene            2309..3295
                     /locus_tag="QGF01_04015"
     CDS             2309..3295
                     /locus_tag="QGF01_04015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HJN20093.1"
                     /translation="MENKIIQSLGIPFTALISIIFLLMISSFPIGAYVVFNSEINDNI
                     TYEYPMSNLESILSEIGIESSMKFELGDGFILLWSIYLILFTIAILGPKKHFIQVLQS
                     IISHGKYEIKDNYMFPIIKWFSILVLTSTIIILAQEFFGISTEQLGTSNDLIQFFDVT
                     LAPLIEEFLFRGVLIGIPIFLLYSNRFSFQFFIKSLWRPWQNLEIVEKRKGLFVIVIV
                     GIVFGASHIIFGEGWSTGKFAQASIGGIIIGWVYFRYGFAPAIIIHWATNYFIFSYGY
                     LVADLNQFTIENAFTHPFVSMIEILLIVTGIISIIILVTNYIMEKRCVIEKE"
     gene            complement(3299..3931)
                     /gene="cofC"
                     /locus_tag="QGF01_04020"
     CDS             complement(3299..3931)
                     /gene="cofC"
                     /locus_tag="QGF01_04020"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HJN20094.1"
                     /translation="MKTSAIIPVKSFSKAKTRLNLPQIQKEDLCIKMFDEVLRTISNC
                     KSIDKILVVSKDEQAMKLSKQYNTMQIFDHETGVNDAMDLANQYLIDKKYDCSIIFPQ
                     DIPIMKPSDIDNIFSFFKSDMGVIIVPSRQFNGTNALLQSPVGIMNTQYDRGSYRYQL
                     DAAKTATKNTSIALIRRIMLDIDDSSDLSLMLNQNEKPDFCKQIINLQQS"
     gene            complement(3933..4850)
                     /gene="cofD"
                     /locus_tag="QGF01_04025"
     CDS             complement(3933..4850)
                     /gene="cofD"
                     /locus_tag="QGF01_04025"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HJN20095.1"
                     /translation="MITVLAGGTGSVKIVRGLASQRSDVHVISNVGDNYWLYGLYICP
                     DIDIIIYGLSDLLDQDKGWGLKKDTFGFLRQMEIFGEETWFRIGDRDTATHLTRTNML
                     KNGKSLSDITKWMCEKFAIETKIIPVSDHSIETRLITDKGEMHLQEYWVKYRGKDTVK
                     GIEYMGSDKARPNPEALNSIHDASLVVIAPGNPLTSIGPMIQLKGVRKELIKNKRKVV
                     AVSPIVGDKALSGPAGKYMEAAGLEVSAYGIAKLYADICSHIVIDTKDRMQTKKIEAL
                     GMHVHETSIKMTNKKSEDALASYLLNHIH"
     gene            4960..6258
                     /locus_tag="QGF01_04030"
     CDS             4960..6258
                     /locus_tag="QGF01_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20096.1"
                     /translation="MRTKSVILTTIIASMFIIGTITPVLGSAQLTVTVNPDNDTATAS
                     MKYQRAIFIDYSEGGELANDLRGKDVFVSFSADSSNAGVKNLIGQLNSYILSKNSQAQ
                     ITDLTVHYTATMTGRENSMSIDYKIELLPVIEKFVIRSYSPGSPAIIDVDWRGFGTNG
                     PVAINTAEYGTLEINTPISFIQKFEPELAAAIVAGDADVLTKTILMNADGVKNQPVGN
                     WHFLFDPTGIQADASQHGLAEELAGKVISSYTMGESNLREGRQVEQVYEATFTMDKVY
                     TLKTFESADNALISVIGFATREISGTSEVFGVSPEPPEGYATTSTGEFPAMILYGMAG
                     MAALGGVAMMMMSRRKLSKEKGHMHQTGIDPSQLTGVATSASAGGYQTVRGEAQVKGL
                     DDYAQHKNFYEEDKPQVEEKKESDSPSTKGALPKGWKPEK"
     gene            complement(6261..6536)
                     /gene="rnpB"
                     /locus_tag="QGF01_04035"
     ncRNA           complement(6261..6536)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="QGF01_04035"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            complement(6576..8936)
                     /locus_tag="QGF01_04040"
     CDS             complement(6576..8936)
                     /locus_tag="QGF01_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012544.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HJN20097.1"
                     /translation="MKIENESSGLVRSLSLSQAVMIGVASMIGGAIFVLVGPGISAAG
                     PALIIAFLLNGVITLFTALTYAELGSALPATGGGYKWVREGLPRPNSYLSGWMAWFAH
                     TIAGSLYAVAFGTFLGHLLTSTKIIDDSFGLPLEKIFAVLAIILFTFVNVRGSSHTGK
                     VGSAITFSQLAIIGILVVGALVAMTFANPTWPENFQDFFPNGTTGLILAMGLTFIAFE
                     GYEIIAQAGDEIKRPKKNIPKAILISLGIVISVYVLFTFVFIGGLNPSLVDEPAWQFI
                     GSFGELGIIETAEYYLPFGALIVLAGGFVSTLAALNATTFAASRVSFAMGRNHDLPPM
                     FKKLHTKYRTPYISTICSAIVMMILAVSFDLTMIALAASVMFLFLFAQVNVACITIRR
                     LYSKKIAYGFKTPLFPLIPVIGFALAIIFSVYLLFSQPLSWVIAIVWVVIGFLIYKFY
                     TSKKEKEYNAPLVFNQAPEERKEYRILVLFDNNTATRLTKIATAIANQHNGEISFLDI
                     INVPKQTPLSMADKSDEVGLGVFDDFKKSISHSIRHRYMVRLCHDPTEAILSTVEEQS
                     INLLIVDFSFLRNKRKILSLSTCDIMGVKLHKNFEKDISNLIVSYDKGRHSNSSLEIA
                     HSLSKEYESKIRIVRGITQSPEVEIEIVNKINECMFDLDIKKVQFENVYPKTDNMLVS
                     SELLANFDQDKHGIVFLGAGNQADAAFSPKTLSLANRSKKTVIILRNHLFSEFHTRSI
                     WNLFVTRFKQNKQVYRIYVEILQLFYSIKSNKEIKRYYEDYFESKF"
     gene            complement(8999..9502)
                     /locus_tag="QGF01_04045"
     CDS             complement(8999..9502)
                     /locus_tag="QGF01_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HJN20098.1"
                     /translation="MKINVIMLKQDDPKKCSAAKLVKFGLVSNIRKTSSRTLVLNPFS
                     KKILLKSDKKLVNSITGIDCSWNHSTDTFSKSFSGISRKLPPLLAGNPVNYAKLNKLT
                     TVEALSAALYILGDSETMTSLLAKFNWGHTFYTLNKNLLEDYSKAKSESDIHKICEEY
                     GLSYSYL"
     gene            complement(9569..9642)
                     /locus_tag="QGF01_04050"
     tRNA            complement(9569..9642)
                     /locus_tag="QGF01_04050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9606..9608),aa:Pro,seq:tgg)
     gene            9745..10638
                     /gene="map"
                     /locus_tag="QGF01_04055"
     CDS             9745..10638
                     /gene="map"
                     /locus_tag="QGF01_04055"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HJN20099.1"
                     /translation="MQIEYYEKAGKIAGEVREDVRNKNWVGHTVEEICQYVESEIIKK
                     GAKCAFPVNTSINEIAAHYTAEPNDPKTISDTDLVKIDLGAQINGYIADTAVTVNYNP
                     KYDTLVQTAENALQGAMSMIKSGIKSKDVGRSIQKTITDVGFKPIANLSGHSLDQYTI
                     HAGKTVPNTWTIGSFTFSENEAFACEPFVTTHEGLGYVRNGKIKNIFALVSRKKTKDE
                     DANKLLEYIWTNFNMLPFALRWILKEWEEKEARKLLEFLEKKKAVHSYPILVEANGEP
                     VAQAEHTFIPTTTGVNVTTMV"
     gene            complement(10628..10807)
                     /locus_tag="QGF01_04060"
     CDS             complement(10628..10807)
                     /locus_tag="QGF01_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20100.1"
                     /translation="MTRKIKTVISYTVEQCDSCTELSKRKFSSGDYIFKIVSKCPSCN
                     GEIRITKIFGETITP"
     gene            complement(10807..11928)
                     /locus_tag="QGF01_04065"
     CDS             complement(10807..11928)
                     /locus_tag="QGF01_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1512 domain-containing protein"
                     /protein_id="HJN20101.1"
                     /translation="MDFSNFSIDEFFGMSDDSNPLMMLIWIIPIILFVFYGQRIQLII
                     TSSDIKKKITELEQFRNDSRNSLVEYVKKNLSPKDDVSQKIDRFIEYFTIMPIDVDPN
                     GIIPKIDHFVRSREDVTRKQVRSLFTDIDMMEVTKIQNLLEIATTLQLLHKVVRHLFL
                     TAKKQNNYPLILPLQMILPMIMEHAYALNDAVPTFKHGQPIGDGIGPLIVGGMMLSTK
                     KQNIEIETVYSESELEGRKIILLKAEGPYATVGRPGKATEKLVEKYKPNMMIMIDASL
                     KLEGEETGSTAQGFGAAIGGIGTDRFMIEEIATKQNIPIFSIIIKQSINDAISLLKKE
                     IIDQVDDVKNQIHEMINDNTNPNETILIIGVGNTIGVSQ"
     gene            12034..12594
                     /locus_tag="QGF01_04070"
     CDS             12034..12594
                     /locus_tag="QGF01_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HJN20102.1"
                     /translation="MPQTKPIVSIENVVSSASVDQKMDLNEITRNFPDVEYHPDQFPG
                     LVFRLKTPKTATLIFTSGKMVCTGAKSEKMSKLAVKTVVEQLRKGGIKVKKQAVVEVQ
                     NIVASINLGGKIHLEQAARTLPRSMYEPEQFPGLIHRMLDPKTVILLFSSGKLVCTGA
                     KKEPDVYRSVNNLHSLLEEKSLMHYE"
     gene            complement(12709..12939)
                     /locus_tag="QGF01_04075"
     CDS             complement(12709..12939)
                     /locus_tag="QGF01_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20103.1"
                     /translation="MTITYEDFKKLDLRVATISSIEKIPGKTRILKGQIDLGDETRDV
                     IVGGADFFEPEELLNKTVIVVANLEPKKNCKY"
     gene            13014..13430
                     /locus_tag="QGF01_04080"
     CDS             13014..13430
                     /locus_tag="QGF01_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HJN20104.1"
                     /translation="MTIINSSIDINASIEKVWNIISDLDNEPKFWKGTKESKTISKDG
                     NVVIREIIIAFRDSKCMQKITIYPKEKIYAEFTDGILKGSKTLNLKAKENSLWLEVEW
                     DIKMTGLAGMFTGMIKRHVKGGTEQALELIKLEAES"
     gene            13437..>14155
                     /locus_tag="QGF01_04085"
     CDS             13437..>14155
                     /locus_tag="QGF01_04085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJN20105.1"
                     /translation="MDIILDIFHYVFIAILIGTSAAWIVLIKSMIITFQKTPVLDKFK
                     KIEHDQPKVSIILPARNEEKFIDKCLSSLLNQDYPNYEIIAINDSSDDSTGEIIKKYA
                     KEFPNVVSVDAKMKPEEWMGKNWACMEGYKKATGDLLLFTDSDTKHASSVISLAVSHL
                     LSLKLDALTVIPKMLCLDPITKITLPMISIFLHTRFSALRVNDPTKNTGYFFGSFFII
                     NKTTYDSVGTHEGVKGEIIEDG"
BASE COUNT         4758 a         2386 c         2347 g         4664 t
ORIGIN      
        1 aaaagtattt tttctgctca aaagcaagac acagcattcc tataccacca ccagcaagat
       61 caccagtttc aatattcata gatgttgaat ttgcatatcc atatcgtaat ggaaaacttg
      121 tttttgcaac aagaatctcc aatgcattct ttgcggcagt gatcaaatct tgagagtcct
      181 cttgtgatat cttccctccc atcgcgttat gtgaatcaac aaccagtaca ctttcaaaat
      241 ttctgttttt cgctatctgc tcaatctcag ttttgatata aagtgggaca tcttccatgc
      301 catgtggaga caaagataac aatagaactg cagacttgtc aagacggatt ccaacaacac
      361 gtgccttgtt catttgggca gtgacaggtt ccgtacacat gagacctttt gttgaaacag
      421 aactttgtga taatccatcc aagtaagttt gtacatcctg ttgtgaggga agattaagtg
      481 agtgatcaga tacgctatgc attaccatcg ctgatgaatt catagttttg tagatttggt
      541 ttggaatatc gctaccacct acgggattaa aaggacctgg atggatatcg ggtagaacca
      601 ttcgaaaatc atttttctca tcatttgtat agaaacgaat ttgagaagta gtaatgtttg
      661 atgattttga tgtttcaagt attattgatt ccatatcacg tggatcatta tgtgaaacgg
      721 attgtagata tgcctgtaat aatttatgtg tgctttttat cactggtaat ccagatctgt
      781 ttgtaagata agaccagaaa catgcaacag ctaaaaatat aattccaaat actaatgatt
      841 gtacgtcata aagaattggc cacatgtcca ttggaaccat tacaaaaaac attgctagtg
      901 gttgaatgaa gcataacaca catgattttt tcaaactaat tccaagagtt gttgtcataa
      961 ttccaattct aaaactagaa aaaatgtaca tgccaactgc aacataaaac aaggataatt
     1021 cctttgaaac tatgaaggca ccaactatac caccgactac ggtgattaac cataacacat
     1081 taccaaaaag tgacatgtgt aacgattttg agtattcttt atgtttggtg aaacgagaat
     1141 cagtatattg tgtgacaagc aatattgcaa taacaattgg aatagtgaat aggatttcac
     1201 cactctgaag ataatttaga attactagtg ttgatattat agctgcagtg acaattgaaa
     1261 atattaacga aacataaaat gatgcagggt tgattaatgt gaatgaatat cgtttatgga
     1321 tattttgtat atcgtctgaa tcagacatgt tattttataa ccaaaaatag ttcaagtgcc
     1381 catgaaactg caatcaaact gattggaata gatattatga tctttggatc gattctaaca
     1441 cctctagttt catcctcaaa aaatcttaat aatcctgcac tagatgctgg taatggcgca
     1501 tttttctttt tatctgccat atctgattca gaaatctaaa gttagcataa aaacttttgt
     1561 tattttttat tcagtttatc aataacactg attagtgaat ctattgtttt gatcaattct
     1621 tgttgtttta cgggatcttc ttccttttca agttgttttg atagtttttc taacttgttt
     1681 tgcattattt ttgaggtaga tgttgattcg tgtttattag gaatattttc tgcttcttgt
     1741 ttaggctcct tcccacaatc aatgcataat gcatttccat ccttgataac tctcacaccc
     1801 ttacaatatg gacagggttc tgcaagtaat gtagcaccat tcaggagcat ttctactgcc
     1861 tttcttgtta gatccttaga cataatatta tcaaatcaca ctttgtaaaa aatactagga
     1921 ttgctaaaat ataattgaaa atattcaaga aggcttaata gtgttaacaa gcttaattgt
     1981 tctaactgat ggcagtaatt tgtagtacat gtggacttcc tgaggattta tgtgcctgtg
     2041 gtgatcttga caaagatagt aatcatatca taatacgtct tgaaactaga cgttttaaaa
     2101 aaaagggaac aatgattgaa ggtcttgatc ctaaaatcaa taatcttgaa agtgttggaa
     2161 aggatttaaa aaagaaatat gcttgtggtg gaactgcaaa aaatggttac atctttttac
     2221 aaggtgatca tagagacaca ataaaagata cattgatcaa gctcggattt gcggcagaaa
     2281 cgatagaatt acactagaaa aagtattgat ggaaaataaa atcatacaat cattaggaat
     2341 accattcact gctttaatat caataatttt tttattgatg attagctcat ttcctatagg
     2401 cgcatatgta gttttcaatt cagagatcaa tgataatata acatatgaat atcctatgag
     2461 taatttagaa tctatactat ctgaaattgg aatcgaatca tcaatgaaat ttgaacttgg
     2521 tgatggtttt attctattat ggtcaatcta cctgattttg tttactatag caatacttgg
     2581 accaaaaaaa catttcatac aagtattaca atcaataatt tcccacggca aatacgaaat
     2641 caaagataat tacatgtttc ctataatcaa atggttttct atcctagtat taacatcgac
     2701 aatcataata cttgctcaag aattttttgg tatttctact gaacagttgg gaacatcaaa
     2761 tgatctaata cagttttttg atgtaacgtt ggcaccatta attgaggaat tcctttttcg
     2821 tggtgtattg ataggtattc caatttttct attgtattca aatagattct cattccagtt
     2881 ttttattaaa tcgttatgga gaccatggca aaaccttgaa attgttgaaa aaagaaaggg
     2941 attgttcgta atagttattg ttggaatagt ttttggtgca tcacatatta tttttggtga
     3001 agggtggagt actgggaaat ttgcacaggc atctattggc ggtattatta ttggttgggt
     3061 ttatttcaga tatggatttg ctcctgcaat tataattcat tgggcgacaa actacttcat
     3121 tttctcatat ggatatcttg tcgctgatct aaatcaattt actatagaaa atgcatttac
     3181 ccatcctttt gtgagcatga ttgagatatt attgattgtt acaggaataa tttcaataat
     3241 aatccttgta acaaattaca tcatggaaaa aaggtgtgtt atagaaaagg aataatgatt
     3301 aagactgttg gagattaata atttgtttac aaaagtctgg tttctcattt tgattaagca
     3361 tcaatgaaag atcagatgaa tcatctatat ctagcattat tcttcgaatc aaagcaatcg
     3421 atgtgttttt tgttgcagtc tttgcagcgt ctaattgata ccgataactc cctctatcat
     3481 attgtgtgtt cataatacct acgggagatt gtagtagcgc attagtacca ttaaattgtc
     3541 tagagggcac aattatcaca cccatatcag atttaaaaaa actgaaaata ttatcaatat
     3601 cagaaggctt cattattggt atatcttgtg gaaatattat agagcaatca tattttttat
     3661 caattagata ttgatttgct aaatccattg catcattaac acctgtttca tgatcaaaaa
     3721 tctgcattgt gttatactgt ttacttaatt tcatagcctg ttcatctttt gacactacaa
     3781 gaattttatc aattgattta cagtttgaga tagttctcaa tacttcgtca aacattttga
     3841 tacaaaggtc ttctttttgt atttgaggta agttcaatct tgttttagct ttagaaaaac
     3901 ttttcactgg tattattgca gatgttttca atctaatgta tgtgatttaa tagataagat
     3961 gcaagtgcat cttcagattt tttgttagtc atttttatac tagtctcatg aacgtgcatg
     4021 cctaatgcct ctatcttttt tgtttgcata cgatccttag tatctatcac tatatgtgaa
     4081 caaatatctg catataattt tgctatacca tatgctgaaa cttcaagtcc tgctgcttcc
     4141 atatatttac cagcaggtcc actgagtgct ttatcaccaa ctataggact gactgcaacc
     4201 actttacgtt tatttttaat caattcttta cgaactcctt tcagctgtat catcggacct
     4261 atgctagtca atgggttacc tggtgcaata acaacaagtg atgcgtcatg aatagaattt
     4321 agcgcttccg gatttggacg agccttgtct gaacccatat attcaattcc tttaaccgta
     4381 tcctttcctc tatattttac ccagtattct tgtaaatgca tttcaccctt atctgttatc
     4441 aatcgtgttt ctatcgaatg atcactgact ggaattattt tagtttcaat tgcaaatttt
     4501 tcacacatcc atttagtgat atcactcaaa ctttttccat ttttcaacat attagttcgt
     4561 gtaagatgtg ttgcagtatc tctatcacct attctaaacc atgtttcctc tccaaaaatc
     4621 tccatctgtc gtaaaaatcc aaatgtatct tttttgagtc cccaaccttt atcctgatca
     4681 agcaaatcag ataacccata aattatgatg tcaatatctg ggcaaatgta taatccgtaa
     4741 agccagtaat tatcaccaac attactaatt acatggacat cacttctttg agaagcaagc
     4801 cctctaacaa tttttacaga cccagtacca ccagcaagaa cagttatcat tttatcagaa
     4861 cgttttctca aacatcttta ccaatattac tttttcacta tcaaaatttg aacagggttt
     4921 tgtaacaaag tttattacct tttgagaaaa aaaaacaaag tgcgcacaaa atcagttatt
     4981 ttaacaacaa ttatagcttc tatgtttatc attgggacaa ttacacctgt actaggttca
     5041 gcacaactta ctgtaacggt taaccctgac aatgatactg ctacagcgtc aatgaaatac
     5101 caaagggcta tctttattga ttattcagaa ggtggggaac tagctaatga tttaagagga
     5161 aaagatgtgt ttgtatcatt ttctgctgat tcatcaaatg ctggtgtaaa aaacttgatt
     5221 ggacaattaa attcgtatat tctttcaaaa aatagtcaag cacaaattac agacttgaca
     5281 gtacattata ctgccacaat gacaggtagg gaaaatagta tgtcgattga ttataaaatt
     5341 gaattattac cggtaattga aaagtttgtg attagaagtt actcacctgg aagtccagcg
     5401 atcatagatg ttgattggag aggttttggt actaatggac ctgtcgctat taatacagca
     5461 gaatatggaa ctctggaaat taacacgcct atttcattta ttcaaaagtt tgaaccagaa
     5521 ttagctgcag caatagttgc tggtgatgct gatgttctaa ctaagacaat actgatgaat
     5581 gcggatggag tcaaaaatca accggttgga aactggcact ttttgtttga tccaactgga
     5641 atacaagcag atgcatcaca acatggactt gcagaggagc ttgcaggtaa agtaatttct
     5701 tcatacacta tgggagaaag taatcttaga gaaggtagac aggttgaaca agtctatgaa
     5761 gcaacattta cgatggataa agtatacact ctaaaaactt tcgaatcagc tgataatgca
     5821 ctaatttctg taattggatt tgccactagg gaaataagtg gaacttcaga agtgtttggt
     5881 gttagtccag aaccaccaga gggttatgct actacatcaa ctggagaatt tccagctatg
     5941 attctttatg gtatggctgg tatggcagca cttggtgggg tagcaatgat gatgatgagt
     6001 agaagaaagc tgtcaaaaga aaaaggtcat atgcatcaaa caggtattga cccatcacaa
     6061 ctcactggtg ttgcaacaag tgcaagtgct ggtggatatc aaacagttag aggtgaagct
     6121 caagtaaagg gactagatga ttatgctcaa cataaaaatt tctatgaaga agataaacca
     6181 caagttgaag aaaaaaaaga gtctgactca ccaagtacaa aaggcgcgtt accaaaaggt
     6241 tggaaaccag aaaaataagt gcgtgctagc cgtaaccctc cttctgtttt aacgacattt
     6301 cgctttgcag cctacctgaa cctagtacag gatctttggg ttcggtttgg ctttgcttca
     6361 tgtgggacag gttttcattc cgtctctggg aatctcaggc aaaaccatca tcgtgctcca
     6421 ggtgggattt tttctgtact gttgccaacc atctctgatt atctatctcc agatcacatt
     6481 tcctgtatga agggaggagt ttcctctgcc gtagcagata tcgctcacta gcttgcacct
     6541 aattgtatga aaaatacaag taatttgaat atttctcaaa actttgattc gaaatagtct
     6601 tcatagtatc tcttaatttc cttattggat ttaatcgagt aaaataattg aagaatttca
     6661 acataaatac gatacacttg tttattttgc ttaaatctag tcacaaacag attccaaatt
     6721 gaacgtgtat gaaattctga aaataaatga ttacgtaaaa ttataactgt cttttttgat
     6781 ctattagcta atgacaaggt ttttggacta aaagctgcat cagcctgatt tcctgcaccc
     6841 aaaaatacta taccgtgttt atcttgatca aaatttgcta aaagctcaga tgatactaac
     6901 atgttatctg tttttgggta aacattttca aactgtactt ttttgatatc aagatcaaac
     6961 atacactcat tgattttgtt tacaatttcg atctcaactt ctggagactg cgttatgcca
     7021 cgaacaattc taattttact ttcatattct ttagataaag aatgtgcaat ctcaagacta
     7081 gaatttgaat gacgtccttt atcatatgaa actataagat ttgaaatatc tttctcaaaa
     7141 tttttatgta attttactcc cattatatca catgtagata atgataaaat ttttctttta
     7201 tttcgtaaaa atgaaaaatc aactatcaat aaattgattg actgttcttc aactgtggat
     7261 aagatagcct cagtaggatc atgacaaagt ctaaccatat atcgatgtcg tatagaatga
     7321 gaaattgatt ttttaaaatc atcaaacaca cccaatccta cttcgtcaga tttatcagcc
     7381 atcgataacg gtgtttgttt tggtacattg attatgtcta gaaatgaaat ttcaccatta
     7441 tgctgattag caattgctgt agcgattttt gttaatctag tagctgtatt attatcaaat
     7501 aatactaaaa ttctatactc tttcctttct tcaggtgctt gattaaatac aagtggtgca
     7561 ttgtattctt tttccttctt tgatgtataa aatttataaa ttaaaaatcc aattacaacc
     7621 caaacaattg cgattaccca actaaggggt tgagaaaata acaagtaaac agaaaagatg
     7681 atagctaatg caaaacctat gactggtatt aaaggaaata aaggtgtctt aaatccatat
     7741 gctatctttt ttgaataaag ccgacgtatg gtgatacatg ctacatttac ttgagcaaat
     7801 aaaaatagaa acataacact agctgcgagt gcaatcattg ttaggtcaaa tgagactgca
     7861 agaatcatca taactatagc agaacagatt gtagaaatgt atggagttct atattttgta
     7921 tgtagtttct taaacatggg tggcaggtca tgatttctgc ccatagcgaa cgatacacgg
     7981 ctagcggcaa atgttgttgc gtttagcgct gctaaagttg atacaaatcc tcccgcaagc
     8041 acaattagag caccgaatgg aagataatat tcagcagttt ctattattcc tagttcacca
     8101 aaacttccaa taaattgcca agccggttca tcaaccagtg atgggtttaa gcctccaata
     8161 aaaacaaacg tgaataaaac ataaactgaa attactattc ctaaagaaat cagaatggct
     8221 ttgggtatat tcttctttgg tcttttaatc tcatcacccg cttgtgcaat aatttcatat
     8281 ccctcaaatg caataaacgt caaacccatt gctaaaataa gtccggttgt accattagga
     8341 aaaaagtctt gaaagttttc tggccaggta ggatttgcaa atgtcatcgc tacaagggct
     8401 cctactacta agatgccaat aattgctaac tgggaaaatg taatagcact gccaactttt
     8461 ccagtatgtg aagaacctct aacattaacg aaagtaaata atatgatggc tagtacagca
     8521 aaaatttttt caagcggtaa tccaaatgag tcatcaatta tcttagtaga agttaagaga
     8581 tgacccaaaa atgttccaaa tgcgactgcg tataaactac cagctatagt atgtgcaaac
     8641 catgccatcc aaccacttag ataagagtta ggtctaggaa gtccttctct tacccatttg
     8701 taaccaccac ctgttgcagg caatgcggat cccaattcag cataagtcag tgcagtaaaa
     8761 agagtaatca caccattaag cagaaatgca attattaatg caggtcctgc tgcagatatg
     8821 ccaggtccaa ctagaacaaa tattgcccca ccaatcattg atgcaacacc aatcattact
     8881 gcctgagaaa gtgaaaggct cctaacaagc ccagaagact cattttcaat tttcattatc
     8941 gaccgatttg gtattgcact agaataaatc tgtagttgtt tattatatcg aataaacgtt
     9001 atagatatga gtatgatagc ccatattcct cacaaatttt atggatatct gattctgatt
     9061 tagcttttga ataatcttcc aagagatttt tgtttagcgt ataaaatgta tgcccccaat
     9121 tgaatttagc aaggagtgag gtcatggtct cagaatctcc taaaatgtat agagctgcag
     9181 atagtgcttc aactgtggtt aatttattca gtttagcata gttcacagga tttcctgcca
     9241 atagtggtgg aagttttctt gatattccag aaaatgattt agaaaatgta tccgtcgaat
     9301 gattccatga acagtcaatt ccagttatag agtttacaag ttttttgtct gattttaata
     9361 atattttctt tgaaaatggg ttcaaaacaa gtgtgcgtga agatgttttt ctaatattac
     9421 ttacaagccc aaattttaca agttttgccg cactacactt tttgggatca tcctgcttta
     9481 acataattac atttattttc atatcaaaaa tttgagtatg cttagttaat gtttttcttt
     9541 catactttgt atgatgataa aaaatcaatg ggattggagt gattcgaaca ctcgacctct
     9601 gcgacccaaa cgcagaatca taccaagcta gaccacaatc ccagtgagat acaaaattgc
     9661 cccaatttaa gcagtatcac atgcattttg ctttgatacg atgttacatg gatgatttta
     9721 atatgataca aaattaggtg gcgtatgcag attgaatatt atgaaaaggc aggaaaaatt
     9781 gctggtgaag taagagaaga tgttcgtaac aaaaactggg ttggccatac tgttgaagaa
     9841 atctgtcaat atgttgaatc tgaaattatc aaaaagggag ctaaatgtgc gtttccagtg
     9901 aacacaagta taaatgaaat tgcagctcat tatactgctg aaccaaatga tccaaaaaca
     9961 atctctgata cagatttagt aaaaattgat cttggtgcac aaataaatgg atacattgct
    10021 gatactgcgg ttacagtcaa ctataatcca aaatatgaca cacttgttca aactgcagaa
    10081 aatgctttac aaggtgctat gtcaatgatc aaaagtggta taaaatcaaa ggatgttggt
    10141 agatctatac aaaaaaccat aacggacgta ggttttaaac cgattgcaaa tctaagtggt
    10201 cattcattag atcaatatac aattcatgca ggaaaaacag ttccaaatac gtggacaatt
    10261 ggctcattca ccttttctga gaatgaagca tttgcatgtg agcctttcgt gacaacacat
    10321 gaagggttag gatatgttcg taatggtaaa ataaaaaaca tatttgcact agtctcacga
    10381 aaaaaaacca aagatgaaga tgctaacaag ttactagaat acatatggac aaactttaac
    10441 atgctaccat ttgcattacg atggatttta aaggaatggg aagaaaagga ggcaagaaaa
    10501 ctactagaat ttttagaaaa aaagaaggca gttcattctt atccaattct tgttgaagca
    10561 aatggtgaac ctgtagccca agcagaacac acattcattc ctactacaac cggtgtgaat
    10621 gttaccacta tggtgtaatc gtttctccaa aaatttttgt tattcttatt tcgccattac
    10681 atgaagggca ttttgaaaca attttaaaaa tataatctcc tgatgaaaac ttccgcttgg
    10741 ataattctgt acaagaatca cattgttcta cagtatatga tattactgtc ttaatcttac
    10801 gagtcattat tgagatactc ctatggtatt tcctacacct ataatgagta tagtttcatt
    10861 aggatttgta ttatcattaa tcatttcatg gatttgattt ttcacgtcat ctacttgatc
    10921 aataatttct ttttttagta atgatattgc gtcattaatt gattgtttta taataataga
    10981 aaaaattggt atgttctgtt tagtcgcaat ctcttcgatc ataaacctat cagtcccaat
    11041 tccaccaata gcggcaccaa atccctgtgc tgtactgcca gtttcttcac cctccaattt
    11101 tagtgaggca tcaatcatta tcatcatatt tggtttatac ttttcaacta atttttccgt
    11161 agcttttcct ggtctaccaa cagttgcata tggtccttca gcttttagta aaatgatctt
    11221 tcttccttct aattctgact cgctgtaaac agtttcaatc tctatatttt gtttttttgt
    11281 agatagcatc attcctccaa ctatcaaagg acctattcca tcaccaatag gttgaccatg
    11341 tttgaaagtt ggtacagcat catttagtgc atatgcatgc tccataatca ttggtaaaat
    11401 catttgtaat ggtaatatca aaggataatt attctgcttt tttgcagtca aaaataagtg
    11461 cctaactacc ttatgcaaaa gctgtaatgt tgtagcaatc tctaagagat tttgaatttt
    11521 agtaacctcc atcatatcaa tatcagtaaa taaagatcta acctgttttc tagtaacatc
    11581 ttcacgtgaa cgtacaaaat gatcaatttt tgggattata ccattaggat caacatcaat
    11641 tggcatgatg gtaaagtatt ctatgaaacg atcaattttt tgagatacat catcttttgg
    11701 agaaagattt tttttgacat attcgaccaa agaatttcta gaatcatttc taaattgttc
    11761 gagctcagta attttttttt tgatatcact tgaagtgatg atcaactgta tacgctgacc
    11821 ataaaataca aaaagaataa ttggtataat ccaaattaac atcataagtg ggtttgaatc
    11881 atcactcatt ccaaaaaatt catcaatact aaaattacta aaatccacat agcaaatcat
    11941 tatactataa acataaacta ttcgtcaatt caccacatat gattagtatc gaattagtat
    12001 tgcttttatt atggaatata aaaaaacaca actatgccac agacaaaacc tatagtaagt
    12061 atagaaaatg tggtatcttc tgcgtcagtt gaccaaaaaa tggatctgaa tgaaataaca
    12121 cgtaatttcc ctgatgttga gtatcatcct gatcaattcc caggattagt ttttcgactg
    12181 aaaacaccaa aaactgctac gttaattttt acatcaggta aaatggtatg tacaggtgca
    12241 aaatctgaaa aaatgtcaaa actagctgtt aaaactgttg ttgagcaatt acgaaaaggt
    12301 ggaatcaaag ttaaaaaaca ggctgttgtt gaagttcaaa acattgttgc atcgattaat
    12361 ttaggtggaa aaattcatct tgaacaagca gcaagaactc tacctcgtag tatgtatgaa
    12421 ccagaacagt ttccaggtct tatccataga atgcttgatc ctaaaactgt aattctgtta
    12481 ttttcatctg ggaagctagt ttgtactggt gcaaaaaaag aacctgatgt ttaccgttct
    12541 gttaataatt tacattcact ccttgaagaa aaatctttaa tgcattacga ataatcatta
    12601 atgcaataat agaattattt aatggctgtt ccaggtggca ctttactatc tacagttaac
    12661 caaaatggtt taccgtctac atcagcagcc aaaagcatgc aatcagattc aatatttgca
    12721 attttttttt ggttctaaat ttgcaacaac aatgacagtt ttattcaata attcctcggg
    12781 ttcaaaaaag tcagcgccac caactatcac atctcgtgtt tcgtcaccaa gatctatctg
    12841 gcctttgaga atccttgttt taccaggaat tttttctatt gatgaaatag ttgcaactct
    12901 aagatctaat ttcttaaaat cttcatatgt tattgtcata gtttaattaa ataccacact
    12961 ttaaaattaa tttcgaaatc aagactaata tttaaagtgg ttatatacat aatatgacaa
    13021 taataaattc gtctattgat attaacgctt caatagaaaa agtttggaat ataatctcag
    13081 atcttgataa cgagccaaaa ttttggaagg gtacaaagga atcaaagact atatcaaaag
    13141 atgggaatgt ggttattaga gaaataataa ttgcgtttag ggattctaaa tgcatgcaaa
    13201 aaattacaat atacccaaaa gaaaaaattt atgctgaatt tactgacggg atacttaagg
    13261 gttcaaagac attaaaccta aaagccaaag aaaatagttt atggcttgaa gtcgaatggg
    13321 atatcaaaat gacaggacta gctggtatgt ttacaggtat gataaagaga catgtcaaag
    13381 gtggtactga acaagccctt gaattaatca aactagaagc cgagagttag taattcatgg
    13441 acataatact agatattttc cattacgtat tcattgcaat attaattgga acgtcagcag
    13501 cgtggattgt actcatcaaa tccatgatta ttactttcca aaaaacacct gttttagata
    13561 aatttaaaaa aattgaacat gaccaaccca aagtctcaat aattttacct gcaagaaatg
    13621 aggaaaaatt tattgataaa tgccttagtt cgttgttaaa tcaggattat cctaattatg
    13681 aaattatagc cattaatgac tcctcagatg attctactgg agaaataatc aaaaaatatg
    13741 ccaaagaatt tcctaacgtt gtatcagtag atgcaaaaat gaaacctgaa gaatggatgg
    13801 ggaaaaattg ggcatgtatg gaaggataca aaaaagctac tggtgacttg ttactattca
    13861 ccgactctga cacaaaacat gcctcatctg tcatttcact ggctgtatcg catttattgt
    13921 cactgaaact tgatgcatta acagtaattc caaaaatgtt atgcttagat ccaattacta
    13981 agatcacctt gccgatgatt tctatattct tgcacaccag attttctgca ttacgagtaa
    14041 atgacccaac aaaaaataca ggatattttt ttggaagttt ctttattatt aataaaacaa
    14101 catatgattc agttggaaca catgaaggtg tcaaaggaga gattattgaa gatgg
//
LOCUS       DASCPJ010000087         2016 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001451771, whole genome shotgun sequence.
ACCESSION   DASCPJ010000087 DASCPJ010000000
VERSION     DASCPJ010000087.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001451771"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(14..135)
                     /locus_tag="QGF01_04090"
     tRNA            complement(join(14..49,98..135))
                     /locus_tag="QGF01_04090"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Tyr,seq:gta)
     gene            228..848
                     /locus_tag="QGF01_04095"
     CDS             228..848
                     /locus_tag="QGF01_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20106.1"
                     /translation="MNAIVLESTHKKIGYICVPYKHDNFSVSVKDYWTLSKNFSSIYF
                     VTATFSDDIKPYFPSSTNHYLLGKFNDDADIIKNHKKFMNDSPSFVFSINDELFERNI
                     KQMQRFVSIYYVEFNDQEAISDISNVIVKKDRIQQAGFAHLSVFCENKPKFTFPYSDR
                     IIILEVADDRSPQSICKYCEKTRQDISRKGVVMNNLVSFSLLEKLK"
     gene            883..1296
                     /locus_tag="QGF01_04100"
     CDS             883..1296
                     /locus_tag="QGF01_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HJN20107.1"
                     /translation="MSKPTELGSLKTGSYILLPVSDQPTGDPCRITEYDTSKPGKHGA
                     AKARIVAVGVFDGQKRPHVGPVSMQVHVPLIDKRAGQIVSITGSNFQLMDSETFETFD
                     ADLVDEEIEGKLQQGQDIEYWKVMDRIKIMRIKSS"
     gene            1421..>2016
                     /locus_tag="QGF01_04105"
     CDS             1421..>2016
                     /locus_tag="QGF01_04105"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HJN20108.1"
                     /translation="MKLASLFSGGKDSMYSIYKAKQMGHSIECLITAFPKSSDSKLLH
                     FPAIKLTELQSKTMKIEQIISELNSDETVEQMNALEILIEKAKKDFQIEGIVHGGISS
                     EFQKKSFENICQKNNLKIITPLWMTDPKEYMNELINSGFKFILTSVSSDGLDESWLGK
                     IISSSDLTKISNLSIEYGFNLNFEGGEAETFVIDCPLYS"
BASE COUNT          728 a          291 c          364 g          633 t
ORIGIN      
        1 attgggttgt aagtcccaag cgaaggaatt gaaccttcga caacccggtt tctgtatgcc
       61 tgataggctg gaattcagtt tagcctaaag ccaactacta cagccggaag ctctaccagg
      121 ctgagctagc ttgggacaac attccgaatc attatggtat taaaaaatta tcggatgtat
      181 tttattctat gaaatggagg caatatgatt aaaataagta ggtgattttg aatgctattg
      241 tattggaatc aactcacaaa aagatcggct atatctgtgt accatataaa catgataatt
      301 tttcagtctc tgtgaaggat tattggacgt tatcaaaaaa ttttagttca atttattttg
      361 ttacagctac tttctcagat gatataaaac catattttcc atcatcaaca aatcattatc
      421 ttttgggtaa attcaatgat gatgcagata ttatcaaaaa ccataaaaaa ttcatgaatg
      481 atagcccatc atttgttttt tcgatcaacg atgaactttt tgagagaaat attaaacaga
      541 tgcaaagatt tgtctcaata tactacgtgg aatttaatga tcaagaagct atatctgata
      601 tttctaatgt aattgtgaaa aaagatagga ttcaacaagc tggttttgca catttgagtg
      661 ttttttgtga gaataaacca aaatttacat ttccatattc agatcgtata ataattttgg
      721 aggtggcaga tgatagaagc ccacaaagta tttgtaaata ttgtgaaaag acacgtcaag
      781 acatatccag aaagggtgta gttatgaata atcttgttag tttttcactc cttgagaaac
      841 tgaaatagaa aagttcaaaa gctgctcata tcaattttct acatgagtaa acctactgaa
      901 ttaggatcat taaaaactgg ttcttacata ttacttcctg tatcagatca accaacaggt
      961 gacccatgtc gtattacaga atatgataca agtaaacctg gtaaacatgg tgcagccaaa
     1021 gcaagaattg tcgctgtagg agtttttgat ggccaaaaac gaccacacgt aggtccagtt
     1081 agtatgcagg tacatgtacc tttgattgat aaaagggctg gacaaatagt ttcaataaca
     1141 ggttccaatt ttcaattgat ggattctgaa acatttgaaa cttttgatgc tgatttggtt
     1201 gacgaagaaa ttgaaggaaa attgcagcag ggccaagata ttgaatattg gaaggtcatg
     1261 gatcgaatta aaataatgcg cattaaatca tcatagcttg gatgctgatg atgattggaa
     1321 aagccatctg aattctgatg aaggttatga gacatgaagc atccttattt tctatattta
     1381 cacaaaaaaa tgaaatatgc tttgaattaa atcatttagt atgaaattag cgtcattatt
     1441 ttcaggtgga aaagatagca tgtattcgat ttacaaagca aagcagatgg ggcatagtat
     1501 cgaatgtttg attacagcct ttccaaaatc ttctgacagc aaactattac attttccagc
     1561 tataaaatta acagaattgc agtcaaaaac aatgaaaatt gaacagatta tctcagaatt
     1621 aaattctgat gaaacagtag aacaaatgaa tgcccttgaa atattaatag aaaaagcaaa
     1681 gaaagatttc cagattgagg gaatagtaca tggcggtata tctagtgaat ttcaaaaaaa
     1741 atcttttgaa aatatatgtc aaaaaaataa cctaaaaata attactcctt tatggatgac
     1801 agatccaaaa gagtacatga atgaacttat caactctggg ttcaaattta ttttaactag
     1861 tgtatcatct gatggattgg atgaatcatg gttaggaaaa attatttcca gttcagatct
     1921 gacaaagata tctaatttat ctatagagta tggatttaat ctaaattttg aaggtggtga
     1981 agcagaaacg tttgtaatcg attgtccact ttactc
//
LOCUS       DASCPJ010000088         5943 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001466684, whole genome shotgun sequence.
ACCESSION   DASCPJ010000088 DASCPJ010000000
VERSION     DASCPJ010000088.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001466684"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="QGF01_04110"
     CDS             <1..282
                     /locus_tag="QGF01_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HJN20109.1"
                     /translation="CVTKKAELREKPQPREKAEPKKAELREKPQPREKVELREKPQPR
                     EKVELREKPQPREKVELREKPQPREKLQPREKAAQKEDASHFSTIKQSS"
     gene            complement(357..1598)
                     /locus_tag="QGF01_04115"
     CDS             complement(357..1598)
                     /locus_tag="QGF01_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HJN20110.1"
                     /translation="MPKNVKISGVSPFGSSTGTFEVEISILVSKPSKDDIKNKTFDSL
                     WKDNYHLRVVNGNFSEVLGDGSNAIPDKVFSLESIWILITDQFSDIHTSFEVKLSQSA
                     KDDSTTTKVSDDGKRQKSKERDVSTQKTQKDRGYFEKRYIPVKGQPGPMGPAGDKGHA
                     GPPGPTGDKGPAGSKGSTGDKGDKGDRGITGPTGDKGPTGPTGDKGPSGDKGIQGSKG
                     DKGHNGDKGDKGDKGITGPTGDKGPTGPTGPPGPTGDKGIQGPPGEKGITGIHGPPGD
                     KGPQGPQGEAGDKGLTGIPGQQGERGIPGPMGLQGERGPTGKSGEPGPLGPQGIQGQQ
                     GERGLTGPSGTPGDAGTIGDKGPMGQPGPRGPPGPPGEKGPTGGISEDTKTLIKELLE
                     VLASKNIITTEEQIKLSSYLY"
     gene            1720..2976
                     /locus_tag="QGF01_04120"
     CDS             1720..2976
                     /locus_tag="QGF01_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20111.1"
                     /translation="MKKQSNPSSDDPDKIPEFFLDKDKIEKTEDVLEEKEKSETKPAK
                     KNFFKKRSNSDKKSFNTKLAKQETSLTDSDFNRKKLFSKGINQMADEKLEEASRTFEM
                     ILRIDPNDVYALLKLGYARFHLEDYTESMRAYDKVLDIDVTNADAWNLKSLVYYERKS
                     YGKALDCAEKSIDSDPTFGMAWYNRACYLSLLNEVPESLDALIHSIEIDVKSAKRAVK
                     DKDFMNVRLEEGFKRIIEVVVIESLRQGYHTIGAIVWTTFLDKVDVIKCLDRLMIKGL
                     VVKHEQRKVWSTIDTYDLVQELTSKISVTRRGLLGIAKTTLPQPVKSLKVLAEAIQLI
                     KTSIEDEELEKSIEYCNFFIDPEKCGQEMINDFLEEHREIRLFKIRLKDKGLDFLQDN
                     KKKMLLMLDDMEVSVTKKLRSELPQN"
     gene            complement(2984..3784)
                     /locus_tag="QGF01_04125"
     CDS             complement(2984..3784)
                     /locus_tag="QGF01_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HJN20112.1"
                     /translation="MSNEIIQSTIDSDTVRSKINDGSVRLVDVRREEEYNQGHIINAI
                     NLPLATLLADDSPENVVKLIEKMGIADDTTVIVYDDTFGALASRVAWTLQYIGHSDVA
                     LLDVTYSKWNELGLETDTKKPNIKSVKHSLNLKPDIIATAEYLEKAKEKNDVVLIDNR
                     ERLNFLEQHIPGAINIPYRTLGTDGKILRTKESMRNFLKNRGVSENAEIITYCGSVGT
                     LSGLAFFALKSIGIPNVKLYVRSFKEWKSLEKQTDKQENASYWDLSAE"
     gene            complement(3781..5583)
                     /locus_tag="QGF01_04130"
     CDS             complement(3781..5583)
                     /locus_tag="QGF01_04130"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HJN20113.1"
                     /translation="MAQQLRPSKSTSKKSNVDWSRREESDNFASMVKLYRQGKIDHDS
                     FRRFRLQHGAYGTRMTDDYAMVRIKIPAGQIYPYQLEKIAQLSEMYSIGSAHVSTREN
                     MQLHWVVLEDVSEIMHGLADVGLTSREACGNTVRNVMCSPLSGVCDNEAFDATPYAIA
                     TAKFLLRNPLNQSLPRKFKFNFSCCENHGMTRIVDVGLIPQIREIDGKNQRGFKIFLG
                     GGLGNKSYVGHQLEDFTSDEDLLYTSIAVLQIFDRMGDRKNIARNRMRYLVHEMGWEK
                     FQGLILKQRAIVRTTQSVIVRLNAKQSSNEIKRPISVSDESGSKPDGYARWLKSTTYK
                     QKQEGHTSVFITLEAGDITANQIRAIAQITRDFSAEGFARNGFSQDIVLRYVHESDLP
                     KLYSRLLEVGLANSGSLTMVSPVGCSGTSSCNLALTNSHRLAKEIQRKFLELKLDEDD
                     DLRNATIKISGCPNSCGQHEIATVGFSGGGARLGKDMYPYYNMSLGGRSDEKAMLGVF
                     CMKVPVKRTIPVLLKIIELFKKNKQQNDTLSKWIDRIVLGNESSEIKSVRDIKKILSP
                     LVVPPTKDNEPDFYLDYGSDTSYHTVTGKGECAA"
     gene            5742..>5943
                     /locus_tag="QGF01_04135"
     CDS             5742..>5943
                     /locus_tag="QGF01_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HJN20114.1"
                     /translation="MQESVNDQRPQKIFADAKEVEITRAIAEEFFSVLQDRAESDVII
                     IGAGPAGLTAARELSLMGYKILV"
BASE COUNT         1895 a         1117 c         1012 g         1919 t
ORIGIN      
        1 tgtgtgacaa aaaaggcgga gctaagagaa aagccccagc caagagaaaa ggcggagcca
       61 aaaaaggcgg agctaagaga aaagccccag ccaagagaaa aggtggagct aagagaaaag
      121 ccccagccaa gagaaaaggt ggagctaaga gaaaagcccc agccaagaga aaaggtggag
      181 ctaagagaaa agccccagcc aagagaaaag cttcagccaa gagaaaaggc ggcgcaaaaa
      241 gaagacgcta gtcacttttc cacaattaag cagtcgtcat aagcgacgat tgttttttac
      301 ttttttctac aaatttttct aagaaaatat aatactcatt attttaaatc aagaagttag
      361 tatagataac ttgaaagctt gatttgttct tctgtagtta tgatattttt tgatgcaagt
      421 acttctagta attctttgat caacgtttta gtatcctctg atattcctcc ggtaggtcct
      481 ttttctccag ggggtccagg tgggcctctt ggtcccggct gtcccattgg tcccttatca
      541 ccaatagtgc cagcatcgcc aggtgttcca gaaggaccag ttaatccacg ttctccttgc
      601 tgtccttgaa ttccttgtgg acccagtgga ccaggttcac ctgatttacc tgtgggacca
      661 cgttcacctt gtaatcccat tgggcctggg attccacgtt ctccttgctg accaggaata
      721 ccagtaagtc ctttatctcc agcttcacct tgtggacctt gaggtccctt atcaccaggg
      781 ggaccatgta tacctgttat tcctttttct ccaggaggac cttgaatacc tttatcacca
      841 gttggtcctg gcgggccagt tggacccgta ggtcctttat cgccagttgg accagtaatt
      901 cctttgtcac ctttatcacc tttgtcacca ttatgaccct tgtcaccttt ggatccctga
      961 atacctttat caccagatgg tcctttatcg ccagttggac ccgtaggtcc tttatcgcca
     1021 gttggaccag taattcctct atcaccttta tcacccttat cacctgtcga ccctttagat
     1081 ccagcaggtc ctttatcgcc agttggtcct ggtggaccag catgtccttt atcaccagca
     1141 ggacccattg gacctggctg acccttgaca ggaatatagc gtttttcaaa ataaccacga
     1201 tctttctgtg ttttttgggt actaacatct ctctcttttg acttctgtct ctttccgtca
     1261 tcagaaactt tagttgtagt actgtcatct ttagccgatt gcgataattt aacctcaaat
     1321 gacgtatgta tatctgaaaa ttggtcagta attagaatcc atatcgactc taaactaaac
     1381 actttatctg gaattgcatt agacccatca cctaaaactt cagaaaaatt tccatttaca
     1441 actctcaaat gataattatc tttccataaa gaatcaaacg ttttattttt gatatcatcc
     1501 ttcgaaggtt tagatactaa tatagaaatt tcaacttcaa atgtgcctgt cgaggaacca
     1561 aatggtgata cacctgaaat tttaacgttt tttggcaata atgtcagtgt ctatatcgag
     1621 tatttgtata tattctgtac tataaaatat acccaataaa caacaaatcc gaatatcatg
     1681 aaatagtatt tatctagaga tactatcatt tctagcaaga tgaagaaaca atcaaatcca
     1741 tcatctgatg atcctgacaa gatacctgag ttttttttgg acaaggataa aatagaaaaa
     1801 actgaagatg ttttagagga aaaggagaaa tccgagacca aaccagcaaa aaagaatttt
     1861 tttaagaaaa gatcaaattc ggataaaaaa tcatttaaca caaaattagc aaagcaagaa
     1921 actagcctaa cggattctga tttcaatcgt aaaaaactgt tcagtaaagg aattaatcaa
     1981 atggctgatg agaaacttga agaggcatca cgaacatttg aaatgatttt aagaattgat
     2041 cctaatgatg tttacgcact tctgaaatta ggatatgcca gattccattt agaagattat
     2101 acagaatcta tgagagcata tgataaagtt ctagatatag atgtcacaaa tgcagacgct
     2161 tggaatctta agagtttagt atattatgaa cgaaagagtt atggaaaagc attagactgt
     2221 gcagagaaat caattgattc tgatcctaca tttggcatgg cctggtataa tagagcatgt
     2281 tatttatcat tacttaacga agttccagaa tcacttgacg ctcttattca ctcaattgag
     2341 attgatgtaa aaagcgcaaa gcgtgcagta aaagacaaag attttatgaa tgtaagatta
     2401 gaggaaggct tcaaacgtat catagaagtt gttgtaattg aatcattaag acaaggttat
     2461 cataccattg gtgcaatagt ctggactaca tttttggata aagttgatgt gataaaatgt
     2521 ttagacagat taatgataaa aggtctggtt gttaaacacg aacaaagaaa ggtttggagt
     2581 actattgata catatgatct tgttcaggag ttaacaagta agataagtgt aacaagaaga
     2641 ggtttactgg gaattgctaa aacaacatta ccacaaccag taaaaagtct gaaagtgtta
     2701 gcagaagcca tacagttgat aaaaacatca attgaagatg aagaattaga aaaatcaatt
     2761 gagtattgta acttctttat agatccagaa aaatgtggtc aagaaatgat aaatgatttc
     2821 ttagaagaac atagagaaat aagattattt aaaataagat taaaggataa aggcttagat
     2881 ttcttacagg ataataagaa aaaaatgcta ttaatgcttg atgatatgga agtttctgtt
     2941 actaagaaac tacgtagtga attaccacaa aactaatttt agattattct gcagaaagat
     3001 cccaataact tgcattttcc tgcttatcag tctgcttttc aagacttttc cactccttaa
     3061 aggaacggac gtataatttc acatttggaa tacctattga ttttaaagca aaaaatgcca
     3121 gacctgataa tgtaccaaca ctaccacagt atgttattat ctcagcattt tctgaaactc
     3181 ctctattttt caggaaattt ctcatacttt ctttagttct tagaattttt ccatcagttc
     3241 ccaatgttct atatggtatg ttgatagcac ctggaatatg ttgctccaaa aaattcagtc
     3301 tttctctgtt atctatcaat acaacatcat ttttttcctt tgctttttct aaatattcag
     3361 cagtagctat aatatctggt tttagattta gtgaatgttt tacagatttg atatttggtt
     3421 ttttagtgtc tgtttctaaa ccaagctcat tccatttgga atatgtaacg tctaataatg
     3481 caacatcact atgacctatg tattgaagag tccatgcaac tctagatgct aaagcaccaa
     3541 aagtgtcatc ataaacaata actgttgtat cgtcggcaat tcccattttt tcaataagtt
     3601 ttacaacatt ttctggagaa tcatcagcta aaagagttgc aagtggtaag tttattgcat
     3661 taataatatg accttgatta tattcttctt ctcgtctaac atcaaccaat cttacggaac
     3721 catcattaat ttttgaacga actgtatctg aatcaatagt tgattgtatt atttcattac
     3781 tcaagcagca cattcacctt ttccagtaac tgtatgataa cttgtatcac ttccgtagtc
     3841 taaataaaag tcaggttcat tatcttttgt tggaggaaca actaatggtg aaagaatttt
     3901 ctttatatct ctcacagatt ttatttcaga cgattcatta ccaagtacaa tcctatcaat
     3961 ccattttgaa agagtatcat tttgctgctt atttttctta aagagttcaa ttatttttaa
     4021 taaaacagga attgtccgtt ttacaggaac tttcatacag aaaactccaa gcattgcttt
     4081 ttcatcagaa cgtccaccga gactcatgtt ataataagga tacatgtctt tacccagtcg
     4141 agcaccccct ccagagaaac caactgtagc gatctcatgc tgtccacaag aattaggaca
     4201 tccactaatt tttatggttg catttcttaa atcatcatct tcatccaatt ttaactctaa
     4261 aaattttcgc tgaatttctt ttgccaatct atgtgaattg gtcaatgcaa gattacaaga
     4321 tgatgtacct gaacagccaa caggtgaaac catggttaat gaacctgagt ttgccaagcc
     4381 tacctcaagt aatcgagagt aaagttttgg tagatcactt tcatgtacgt atcgtaagac
     4441 aatatcttga gagaacccgt ttcttgcaaa accttcagca gaaaaatctc tagtaatctg
     4501 agcaatcgca cgaatctggt ttgctgttat gtccccagcc tctagtgtta tgaaaacaga
     4561 agtgtgtcct tcttgttttt gcttatatgt tgtagatttt aaccaccgtg catatccatc
     4621 aggttttgat ccagattcat ccgaaacaga aataggtctt ttaatttcat ttgatgactg
     4681 tttagcattc aatcttacaa tcactgattg agtggttctg acaatagctc tttgttttag
     4741 aattagacct tgaaattttt cccatcccat ttcatgaact agataacgca ttctattcct
     4801 tgctatattt tttctatccc ccattctatc aaaaatttgt agaaccgcga tagatgtata
     4861 caaaagatcc tcatcagatg taaaatcctc aagttgatga cctacatacg atttatttcc
     4921 tagacctcca ccaagaaata ttttgaaacc acgttgattt ttaccatcaa tctctcttat
     4981 ctgtggaatc aatccaacat caactatcct tgtcattcca tggttctcac agcaagaaaa
     5041 attaaatttg aatttacgtg gaagtgactg atttagtgga tttcttagaa ggaactttgc
     5101 agttgcaatt gcatatggtg ttgcatcaaa cgcttcatta tcacaaactc ctgataacgg
     5161 actacacatg acatttctaa ctgtgttacc acacgcttca cgagatgtaa gtccaacatc
     5221 tgcaagtcca tgcattattt ctgaaacatc ttctaataca acccagtgta attgcatatt
     5281 ttctctagtt gatacatgag cactgccaat tgaatacatt tcacttagct gagcaatttt
     5341 ttctaattgg taagggtaga tctgaccagc aggaatttta attctgacca ttgcataatc
     5401 atctgtcatt cttgtaccat atgcaccatg ttgtagacga aatcgtctaa aactatcatg
     5461 atcaatttta ccttgacggt atagctttac catactagca aagttatctg attcttctct
     5521 tcgtgaccaa tcaacatttg actttttaga agtcgatttt gatggtctta gctgttgtgc
     5581 catctttaga tttgttattc aactacggat ataaattttt gataatagga attttaacta
     5641 gtataatgaa taatttaggt aattgttgcc gactagtatt cattagtgtt gcctaacttt
     5701 caaacaagaa aaattatatg cactcattat attgtatttt tgtgcaggaa tcagttaatg
     5761 atcagcgacc acaaaaaatt ttcgctgacg caaaagaagt tgaaataaca cgtgcgattg
     5821 ctgaggaatt cttctctgtt ttacaagata gagctgaatc tgatgtgata attattggtg
     5881 caggacctgc tggattaaca gctgcgcgtg aattatcatt gatgggctac aaaattttag
     5941 tta
//
LOCUS       DASCPJ010000089         1626 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001488318, whole genome shotgun sequence.
ACCESSION   DASCPJ010000089 DASCPJ010000000
VERSION     DASCPJ010000089.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001488318"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="QGF01_04140"
     CDS             <1..604
                     /locus_tag="QGF01_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HJN20115.1"
                     /translation="MKKSNVNRQRSDKNKKLLNSILLRDAGNKYPNVKPINELHAMNF
                     SCIVDMPVEIGISNILKMKTFNAGGLTDYEEKAKVAAQAMSTENAIYVHLKGPDEFGH
                     DGDAIGKMKNIEEIDKRFFGTLLSNIDISKIAVMISADHSTPCINKGHSDDPVPLLIS
                     GDMIVNDNSQRFTEEDGKKGTIGLIEGASVVKTGIDLFKT"
     gene            complement(594..1154)
                     /locus_tag="QGF01_04145"
     CDS             complement(594..1154)
                     /locus_tag="QGF01_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HJN20116.1"
                     /translation="MDRFMLHLKNSRYLPKDANTVLINSRDLIYGMTAIIRDCRISSK
                     FVELDISVHKNNLELLLEKLTSIGKTDNARLIVEEEIEKGQLVEDGISYFNNERFWES
                     HESLEGAWKQSKGSEKDLIQGLILIAAALVHYQKNEDEICLSVLGRALDKLHDKSGQY
                     CKINVDDVKQKVIEMLDKKTIFIFTF"
     gene            1234..>1626
                     /locus_tag="QGF01_04150"
     CDS             1234..>1626
                     /locus_tag="QGF01_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20117.1"
                     /translation="MSMIQTLNDENNSFLSRREITCDFKGLGGKLKKMEAVEMITKEY
                     KLEGKFVIPIKLHNHTGQSKITGVFFVYEDEKLAKRHVNPTIFKRLDKAKAKDAEPAK
                     EDTKADAEPAKEDTKADAEPAKEDTKADA"
BASE COUNT          602 a          230 c          280 g          514 t
ORIGIN      
        1 catgaaaaaa agtaatgtaa atagacaaag atcagataaa aataaaaaat tactgaatag
       61 catactgtta agggatgcgg gtaacaaata ccctaatgta aaacctatta atgaattaca
      121 tgcaatgaac ttttcgtgta tagttgatat gccagtagag ataggtattt ccaatattct
      181 taagatgaaa acattcaatg ctggtggact tacagattat gaagaaaaag ctaaggttgc
      241 ggcacaggcg atgagtactg agaatgcaat atacgtacat ttgaaaggac ctgatgaatt
      301 tggacatgat ggtgatgcaa ttggtaaaat gaaaaatatt gaggagattg ataagagatt
      361 ttttggcaca ttattgagta atatagatat ctcaaagatt gctgtaatga tttcagcgga
      421 tcattctaca ccatgtatta acaaaggaca tagtgatgat ccagtaccac tactaatttc
      481 tggtgatatg atagtaaatg ataattctca acgttttact gaagaagatg gtaaaaaagg
      541 tacgattgga ttgattgaag gtgcaagcgt agtgaaaact ggaatagatt tatttaaaac
      601 gtgaaaatga agattgtttt tttatctaac atttcaatta ctttttgttt aacatcatca
      661 acattaattt tgcaatattg tcctgattta tcatgtaatt tatctaatgc acgaccaagc
      721 acagatagac atatttcatc ttcatttttt tgataatgta ctagtgctgc agcaattagt
      781 atcaatcctt gaattagatc tttttcacta cctttggatt gtttccatgc accttctagt
      841 gattcatggg attcccaaaa tcgttcatta ttaaaataag aaataccatc ttcaactaat
      901 tgtccttttt caatttcctc ttcaacaata agccgagcat tatcagtttt ccctattgaa
      961 gttaatttct ccaatagtaa ttcaagatta tttttatgta cagaaatatc taattctacg
     1021 aatttgcttg atattctaca atcacgaata atagcagtca taccataaat tagatcccgt
     1081 gaattaatga gtacagtatt tgcatcttta ggtaaatatc tcgaattttt taaatgtagc
     1141 ataaacctat ccatgctttt tttctatggt aatttcataa atttgttgat attagattga
     1201 agatttatat gaaatactca aagggttcta aacatgtcaa tgatacaaac gttaaacgat
     1261 gaaaataact catttttgtc aagaagggag attacatgtg atttcaaagg cttgggtggt
     1321 aaattaaaaa aaatggaagc tgtagaaatg attacaaaag aatacaaatt agagggaaaa
     1381 tttgtcattc ctataaaatt acataatcat acaggtcaat caaagattac tggtgtattt
     1441 tttgtttatg aagatgaaaa attggcaaaa agacatgtta acccaacaat cttcaagcgt
     1501 ttagataaag cgaaggctaa agatgccgaa ccagcaaagg aagatactaa agcagatgcc
     1561 gaaccagcaa aggaagatac taaagcagat gccgaaccag caaaggaaga tactaaagca
     1621 gatgcc
//
LOCUS       DASCPJ010000090        10981 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001488502, whole genome shotgun sequence.
ACCESSION   DASCPJ010000090 DASCPJ010000000
VERSION     DASCPJ010000090.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001488502"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="QGF01_04155"
     CDS             <1..239
                     /locus_tag="QGF01_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20118.1"
                     /translation="LSLIKLLYQLKHQPMIFSDLYTKSMIRMKKSFLNYLHFCVNYKF
                     ITKKSVGQNVVYSITEKGLTMLNLFTQNHDGNDQ"
     gene            273..998
                     /locus_tag="QGF01_04160"
     CDS             273..998
                     /locus_tag="QGF01_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HJN20119.1"
                     /translation="MKSEFSDTNIHEIKKVELNTPIIFAGFVGAGLVGPLSVGCIIDK
                     LEMEEIGYLRSKHLPPSTVFIQGRLRHPFRLYSNHDGTICAIICEVTLRLEGLYEIVS
                     SILDWAEKKGSHEIVILDGVASTTHDKKTFCAAEEDLCRVMEDNGISMIPQGFITGVA
                     GGLLNECLVRKIQGVTLLVKANDKRPDPIASATLVDAVNRTYDMNIDTSDLRKEERKI
                     GVDFKELSDKYTEHKKMNSSMYM"
     gene            1041..2063
                     /gene="purM"
                     /locus_tag="QGF01_04165"
     CDS             1041..2063
                     /gene="purM"
                     /locus_tag="QGF01_04165"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HJN20120.1"
                     /translation="MVTTYKKVGVDITEIKKTQNVIGKIISSTYNSQKLAKVEHGFGH
                     YAGIVQIPGKKFLATHTDGVGSKIIIANMLKKYDTIGIDCIAMNVNDIICVGATPISF
                     VDYIAANKNDQRVFKQIVKGLAYGAKLAQVPIIGGETAIMPDLFSGNKFAMDLAGTVV
                     GITSKRKMVFGKSIKTGDVIIGIKSSGLHSNGYTLARNVLFPKYKLNDKIKGIGHLGR
                     ALLKPTEIYVKPVLDALTKCKIHGLAHITGGSFTKLLRLKNIGYDLDNLPKPPPLMQL
                     IEDCGVGNREMYKTFNMGIGFCIVSPKNNVTKIQNIFKKYGFTSYQIGKIISKKGVFI
                     NSTKIT"
     gene            2133..3569
                     /locus_tag="QGF01_04170"
     CDS             2133..3569
                     /locus_tag="QGF01_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HJN20121.1"
                     /translation="MDVEIASHFSMRSFGIGMAALLVLLVMLFTLPEYVGLELTITMM
                     ATLGVLIGMYVILITEVVHRTALALFGALVMLIVLFTTGVLDPHDSVDFVIGAIDFNT
                     IGLLLGMMVIVGILGETGIFQYIGIKAAKISNGNVWKLMILLAVITAVGSAFLDNVTM
                     VLLMVPVTISVCRILNINPISLILAQIFASNIGGATTLIGDPPNIMIGSAAGIDFMSF
                     AYHMTPEIIITMAVTIVLFKFMFRKDLKQKPENIEKLQALDEKKEIKDTMLLKKSAII
                     LGAVIIMFMLHGMLGLEVSIIALGGAAVLLVVTGKQPYIALKHVEWPTLLFFCGLFVI
                     VGGVEVSGALELLAHNILEITQGELGTTMFTITMVSAFASAFVDNIPFTATMIPIIEN
                     ISLDPAFAHNLTAFDYNPLWYALAFGADLGGNGTLIGASANLVAIAIAEKFGYRIFFR
                     EFLIKGLPLMVITTLVAFGAFYLRVLYL"
     gene            3610..4104
                     /locus_tag="QGF01_04175"
     CDS             3610..4104
                     /locus_tag="QGF01_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20122.1"
                     /translation="MTKTDDESTSSDMLVNHLLGAKKKHDEDKNVEIEEVEHLDKGTD
                     YLVSFLVGQGIQVSQPKKATKQKPPGLEELKKKFVENLVNEGYVTVNDAGRTVLTENG
                     KKFLDSKTKSADTKVAIAKLKNLQFRNELDRQNKRLANKAKKGLKKKIKGLKKKISRK
                     SKRR"
     gene            4156..5736
                     /locus_tag="QGF01_04180"
     CDS             4156..5736
                     /locus_tag="QGF01_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HJN20123.1"
                     /translation="MKNTNFSKACSEISQSLLQIIEPTKKQTKLEIKKICTKYSLERI
                     PKNHEILSTVDGKSYEKLKKVLLKKPVKSASGVTVIALMPKPFACPHGRCTYCPGGVE
                     FNTPNSYTGKEPVTINAIKNQYSPNIQITSKLNQLQLFGHDTSKIELVIVGGTFLFMP
                     KDYQNNFIKSCYDALNGFVSNDLQTAMKNNETTKNRSVGFTIETKPDYCKKEHIDLML
                     SYGVTRVEIGVQSLHERVYKIVNRGHDYNDVIESFQISKDSGYKIVAHMMPGLPTITP
                     KEDINDFKKLFKDSNFKPDMLKIYPSLVLENTALYNDFIAKKYTPYSDEEMLNVLTET
                     KKIVPKWVRIMRVQREITPNEIQAGPKSGNLRQMIHKRLEEQGYACKCIRCREAGLSR
                     KNMPSEVLKLNREEYDSSSGKEVFLSFDDKDETIYGFLRLRKPSKMAHRNEITENSCI
                     VRELHIFGKSIEIGKHEENSIQHSGLGRKLMNEAEKISREDFDARKILVISAIGTREY
                     YKKLDYRLDGPYMSKQLR"
     gene            5740..7341
                     /gene="lysS"
                     /locus_tag="QGF01_04185"
     CDS             5740..7341
                     /gene="lysS"
                     /locus_tag="QGF01_04185"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HJN20124.1"
                     /translation="MTEQKIIGKGTWIDKLAHEVIEREKHLGRNIDGVIRVESGLGAS
                     GIPHIGSLGDAVRAYGIKLALENFGYKSELITYSDDLDGLRKIPEGLQVKEENIGKRV
                     STIEDPYGKCHDSYGSHMSSLLLEGLDKLGIKYVHKTAHDTYKNGILKEQIHKILMNS
                     KKIGDKIEELTGQTKFQNILPYFPVCQNCDKLYTTESYEYVENEKKVMYRCKDSEIGS
                     DKHILNGCGHEGEADITNGLGKLAWKVEFAARWEAFDIRFEAYGKDIMDSVKVNDWVA
                     DEVLGFPHPHHVKYEMFLDKGGKKISKSLGNVVTSQKWLNYGTPESILLLLYKRITGA
                     RELGFEDIPALMDEYNELENIYFGKTKIENEAKLVKSKGLYEYVNLLNPPKNSDVHIS
                     YGLLIELCKIFKDDRLERVNSKLIDYGTIKETNSRIEELIKLAGKYSDDFEETKIPAT
                     KIIIDDSCKIALTQLVELLLKNEKTEDLQNSIYTIAKENQVQPKDFFRILYQIILSAN
                     RGPKIGPFIEDIGKKKVADNISKYI"
     gene            7347..7556
                     /locus_tag="QGF01_04190"
     CDS             7347..7556
                     /locus_tag="QGF01_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20125.1"
                     /translation="MAHNEHNKPKRSGSFLLIIAGSILLMLAPTQYPTSKELGILALI
                     SGLIIGSIGFYIKFVKGRKSRKNNA"
     gene            7568..8353
                     /locus_tag="QGF01_04195"
     CDS             7568..8353
                     /locus_tag="QGF01_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20126.1"
                     /translation="MNWFRNTKKNTDNDSEDSKKNDGIDIKYNKTDEPQIENNTVNEH
                     DTLSSKLELVQQEYHKIVHELMTSKKEFNKMKADIQKLNDENDVLTSKIKSGHTELLK
                     INNELKEKNEILKKIMNDYHRQSIVIKETDKTKNELVQIKEEIKKYNNELDNAKTKTN
                     NSVEVQKARNEKNELESEILKKRKEVESAIKELKFIESEMSKIGNYKQTEVIANNQSN
                     TNIVDAASAVVSSLNKKLQITQKELEMVKKNLDIVRKQKKKIP"
     gene            complement(8371..8811)
                     /locus_tag="QGF01_04200"
     CDS             complement(8371..8811)
                     /locus_tag="QGF01_04200"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJN20127.1"
                     /translation="MITNKIRKFMSEQKLGFVATVSADGTPNLSPKGTIIAWNKDTLA
                     FANIRSPQTMKNLKNNNSIEINIIDPFYRKGYRFKGIAEIITSGSKYNKIISHYKISG
                     VKNPIESIVLVKVKQMNEVISPLYDLGYTEDEIKDKWKKYYLSL"
     gene            complement(8851..8925)
                     /locus_tag="QGF01_04205"
     tRNA            complement(8851..8925)
                     /locus_tag="QGF01_04205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8888..8890),aa:Arg,seq:gcg)
     gene            complement(8968..9681)
                     /locus_tag="QGF01_04210"
     CDS             complement(8968..9681)
                     /locus_tag="QGF01_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HJN20128.1"
                     /translation="MVNPRAWLAEGISTFGLVFFGPLSVILAVVAFGEGLSIEAIIMI
                     SLGHGGIIALMVYAFGHISGAHINPAVTIPMMITKKIGIADGVGYIISQIIGGIIATL
                     TLVAILPEIGKPVLWGGHGGPSELLNNSAMSGFMVEIILTFFLVVVIFMTAVHKKAPK
                     SVYGAAIGGMVFLLHLVGVPLTGASMNPARSFAPAIVSGDVGLLEIQWLYWAAPIIGG
                     IIAGVIMNYVFVKKAEQEA"
     gene            9771..10238
                     /gene="bcp"
                     /locus_tag="QGF01_04215"
     CDS             9771..10238
                     /gene="bcp"
                     /locus_tag="QGF01_04215"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HJN20129.1"
                     /translation="MIDEGKTVPMFELSDADGNIVKTKDFKGKKFVVYFYPRDFTPGC
                     TVEADEFSKNYKKFQKLGIDIIGISKDDVESHKKFCKKMSIPYILLADTTTKVSKLFG
                     VWGKKKFMGKEYMGINRSTFLINKKGKIFKVFEKVKPKGHSKEVLDAYVNAEK"
     gene            complement(10257..>10981)
                     /locus_tag="QGF01_04220"
     CDS             complement(10257..>10981)
                     /locus_tag="QGF01_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20130.1"
                     /translation="ARKAAPSKKALESKIAALERKLSNLSKPKPQTQAPPPKPQTQAP
                     PPKPQTQAPPPPPKQQTLPKPQTPPPKPAEKAPPALVYEQWKQVPTKNWNDQKEKVTG
                     YTTASDRYWASLHVQNAPDVPAKNWNDQKAVVTGYTAPGDKYFATRQRMSYHPRTKTF
                     QQSIAASVSGSDSTQTAPPPPPQPESQPQADSGNTSKQQSLEDYEKDYLLRLEQQAEE
                     GSAAAEEAVATTSKKGALPKGF"
BASE COUNT         4001 a         1587 c         2046 g         3347 t
ORIGIN      
        1 ctctatcatt aatcaagtta ctctaccagc taaaacatca acctatgata ttttctgatt
       61 tgtatactaa atcaatgatc aggatgaaaa aatcattttt aaactacctt catttttgtg
      121 ttaattacaa gtttattaca aaaaaatcag ttgggcaaaa tgtcgtatat tctattacag
      181 aaaaaggtct gactatgctc aatttgttta cgcaaaatca tgatgggaat gatcagtgaa
      241 agaataatta gtatgtaaaa attcaaaaaa gtgtgaagag tgaattttca gatactaata
      301 tacatgaaat taaaaaagtg gaactgaata cacctattat ttttgcaggg tttgtgggtg
      361 ccggcttggt tggtccattg tctgtaggat gcataatcga taaactagaa atggaagaaa
      421 ttggttatct tagatcaaaa catttacctc catcaactgt ttttatacag ggacgacttc
      481 gacatccatt ccgtttatac tcaaatcatg atggaacaat ttgtgcaata atttgtgaag
      541 ttactctacg attagagggg ctttacgaaa ttgtttccag cattttggat tgggcagaaa
      601 aaaaaggttc acatgaaatt gtaattctgg atggtgttgc tagtacaaca catgataaga
      661 aaacattctg tgcagctgag gaggatcttt gtcgtgttat ggaggataat ggtatcagta
      721 tgatacctca aggttttatc acaggtgttg cgggtggact gttaaatgaa tgcttagtac
      781 gaaaaattca gggtgttact ttactggtaa aagctaatga caaacgacca gatccaattg
      841 catctgctac tttggtagat gctgttaata gaacatatga tatgaatatt gacacatctg
      901 atcttcgaaa agaagagaga aaaataggcg ttgattttaa agaactttca gataaatata
      961 ctgaacataa aaaaatgaat tcgagtatgt acatgtgaca tgataaaaaa tttaattatt
     1021 gaaaaaaatg aggcagttgt atggtgacta cttacaaaaa agttggtgta gatatcactg
     1081 aaattaaaaa gacccaaaat gtaattggaa aaattatatc atctacatat aactcacaga
     1141 aattagcaaa agttgaacat ggttttggtc attatgcggg aatagttcag atacctggaa
     1201 agaagttttt ggctacacat acagatggtg ttggttcaaa aatcataatt gcaaacatgc
     1261 tgaaaaaata tgatactatt ggtatagatt gtatagcaat gaatgtaaat gatataatat
     1321 gtgttggtgc aacaccaatt tcatttgttg attatatagc tgcaaacaaa aatgatcaac
     1381 gtgtattcaa gcaaatagtc aaagggcttg catatggtgc aaaattagca caggtaccaa
     1441 ttattggtgg agaaactgct atcatgcctg atctattttc aggtaataag ttcgctatgg
     1501 atttagctgg tactgttgta ggaattactt caaagagaaa gatggtgttt ggtaaatcaa
     1561 ttaaaacagg agatgtaata attgggatta aaagtagtgg gcttcattct aatggatata
     1621 cattagcaag aaatgtatta tttccaaaat acaaactaaa tgataagata aaagggatag
     1681 gacatcttgg acgagcttta ctaaaaccaa ctgagattta tgtcaagcct gtattggatg
     1741 cactgaccaa atgtaaaatt catggcttgg ctcatattac tggtggttca tttaccaaac
     1801 tcttacgctt aaaaaatata ggatatgact tggataatct tcccaaacca ccgccactaa
     1861 tgcaattgat agaagattgt ggtgttggaa acagggaaat gtacaagacg tttaacatgg
     1921 gaataggatt ttgtattgtt tcgccgaaaa acaatgtgac caaaattcaa aatattttca
     1981 aaaaatatgg atttacatct tatcaaatag ggaaaataat cagtaaaaag ggggttttca
     2041 tcaactccac aaaaattacg taatctgttt acccaatttg aaatatattt tgagtaaata
     2101 ctgccatata tagggaaaaa tcagaattag taatggatgt agagatcgct tctcattttt
     2161 ccatgagaag ttttggcatt ggaatggcag ctctactagt actgcttgtt atgttattta
     2221 cacttccaga gtacgtagga ctggaactga ccatcacgat gatggctacg ttgggcgtat
     2281 tgattggcat gtacgtaatt ttgattactg aagtggtaca ccgtactgct cttgcattat
     2341 ttggagctct tgtaatgcta attgttctat ttactactgg tgtacttgat ccacatgata
     2401 gtgtagactt tgtaatcggt gcaattgatt tcaacacaat tggtcttctt ttaggaatga
     2461 tggtgattgt tggaattcta ggtgaaacgg gaatttttca atatattgga attaaggctg
     2521 caaaaatttc aaatggaaat gtctggaagt taatgatttt attggcagta ataactgccg
     2581 ttggttctgc atttcttgat aacgttacaa tggttttact tatggtacct gtaacaattt
     2641 cagtctgtag aatactaaat atcaatccca tatcattaat tttagctcag atttttgcat
     2701 caaacattgg tggggcaaca actctgattg gtgacccacc aaacattatg attggctcag
     2761 ctgctggaat tgattttatg tcttttgctt accacatgac accagaaatt attatcacta
     2821 tggctgttac aattgtacta ttcaaattta tgtttagaaa agatcttaaa caaaaaccag
     2881 aaaatattga gaagcttcaa gctctagacg aaaagaaaga aatcaaagat actatgctgt
     2941 taaaaaaatc agctattatt cttggtgctg taattattat gttcatgtta catggaatgc
     3001 ttggtctaga agtatctata attgcattag gtggagcggc agtgctcttg gtggtcactg
     3061 gtaaacaacc atacatagca ttaaaacatg tggaatggcc aacactactt ttcttttgtg
     3121 ggttgtttgt cattgttggt ggtgttgaag tttctggtgc tttagaactt cttgcacata
     3181 acatactgga aattactcag ggagaattgg gtacaacaat gtttactata acaatggtgt
     3241 ctgcatttgc gtcagctttt gtagataata tcccgttcac tgcaacaatg attccaataa
     3301 ttgaaaacat atctcttgac ccagcgttcg ctcataactt aactgcattt gattacaacc
     3361 cgttatggta tgcactagca tttggcgcag accttggtgg aaatggtaca ttgattggag
     3421 caagtgcaaa tcttgtggcg atagcaattg ctgaaaaatt tggttataga atattcttca
     3481 gggaatttct tatcaagggt ttaccactga tggttataac aacactagtt gcctttggag
     3541 cattttacct tagagtactt tacctgtaaa tggatagact ataaattcaa gtatttctct
     3601 agatcgatat tgacaaaaac tgacgacgaa tccacaagtt cagacatgct cgtcaatcat
     3661 ctactaggag caaaaaagaa gcatgatgaa gacaaaaatg ttgaaatcga ggaagtagaa
     3721 catttagaca agggtactga ttatcttgtt tcatttttgg tcggacaagg tatacaagta
     3781 tctcaaccaa aaaaagcaac aaaacaaaaa cctcctggat tagaggaact gaagaaaaaa
     3841 ttcgttgaaa atcttgttaa tgagggttat gtaacagtca atgatgctgg tagaacagtt
     3901 ctcaccgaaa atggtaaaaa atttcttgat agtaaaacaa agagtgcaga tactaaagtg
     3961 gccattgcta aattaaaaaa tttacaattt agaaatgaac ttgataggca aaataaacga
     4021 ttggcaaata aagctaaaaa aggtcttaaa aaaaagataa aaggtcttaa aaaaaagatt
     4081 tctagaaaat caaaacgcag ataagattag atcgcatgta taaggatttt atgttagaat
     4141 ctatcagtat aacaaatgaa aaacacaaat ttttctaagg catgtagtga gatatcacaa
     4201 agtctattac aaattataga acctactaaa aaacagacaa agttagagat caaaaaaata
     4261 tgcacaaaat attcgttgga aagaatacca aaaaaccatg aaattttatc aacagttgat
     4321 ggtaaatcat atgaaaaact aaaaaaagtt ttactgaaaa aaccagtaaa atctgcatca
     4381 ggtgtaactg taatcgcact aatgccaaag ccatttgctt gcccccatgg aagatgtact
     4441 tattgccctg gtggagttga atttaataca ccaaatagct atacaggtaa agaacccgtc
     4501 acaatcaatg ctattaaaaa ccaatacagt ccaaatattc aaatcacttc aaaacttaat
     4561 caattacaat tatttggaca tgatacatcc aaaatagaat tagtcattgt tggtggtaca
     4621 tttcttttca tgccaaaaga ttatcagaat aactttatca aatcatgtta tgatgcatta
     4681 aatggatttg tatcaaatga tttacaaact gccatgaaaa ataatgaaac tacaaaaaat
     4741 cgtagcgttg gatttactat cgaaacaaaa cctgactatt gcaaaaaaga acatattgat
     4801 ttaatgctat catatggcgt gacaagagtt gaaattggtg ttcaaagttt gcatgaacga
     4861 gtttacaaga ttgtaaacag ggggcatgac tataatgatg ttatagaatc gttccaaatt
     4921 tcaaaggatt ctggatataa gatagttgca catatgatgc ctggattacc tacaataaca
     4981 cctaaagaag atatcaatga tttcaagaaa ctattcaaag attcaaattt caagccagat
     5041 atgttaaaga tctatccatc attagtttta gagaatactg cattatacaa tgattttatt
     5101 gcaaaaaaat acacaccata ttctgacgaa gaaatgttaa acgttttgac tgaaacaaaa
     5161 aaaatagttc ctaaatgggt tagaattatg cgtgttcaaa gagagattac acctaatgag
     5221 attcaagctg gaccaaaatc tggtaattta agacaaatga tacacaaacg gttggaagaa
     5281 caaggatatg cttgtaaatg tatcagatgt agagaagctg gactttctag aaaaaatatg
     5341 ccatcagaag ttttaaaact taacagagaa gaatatgatt catcttctgg aaaagaggtt
     5401 ttcctttctt ttgatgacaa agatgaaaca atatatggat tcttacgatt aagaaagcca
     5461 agtaagatgg cacatagaaa tgaaattaca gagaactcgt gtatagttag agaactccat
     5521 atttttggaa aatcaattga aattggaaaa catgaagaaa atagcattca gcattctgga
     5581 ttaggaagaa aattaatgaa tgaagcagaa aaaatttcaa gagaagattt cgatgcaaga
     5641 aaaatacttg tgatcagtgc tattggtaca cgagaatatt ataaaaaatt agattacaga
     5701 ttagatggac catatatgtc aaagcaattg aggtaaataa tgacagaaca aaaaataatt
     5761 ggtaagggta catggataga caaactagcc catgaagtaa ttgaacggga gaaacatctt
     5821 ggacgaaata ttgatggagt tattagagta gaaagtggat taggtgcttc tggtattcct
     5881 catataggaa gtcttggtga tgctgttaga gcgtatggta tcaaattagc tcttgaaaac
     5941 tttggctata aatctgaatt aattacatat tctgatgatc tcgatgggtt acgaaaaatc
     6001 ccagagggat tacaggtaaa agaagaaaat attggtaaga gggtatctac tattgaagat
     6061 ccatatggaa aatgtcatga ttcttacgga agccatatga gcagtttact tcttgaggga
     6121 ttggataaat taggaataaa atatgtgcat aaaacagcac atgatactta caaaaatggt
     6181 atcttgaaag agcagattca caaaatactt atgaatagta aaaaaattgg agataaaatt
     6241 gaagaattaa caggtcaaac aaaattccaa aacattttac catattttcc agtttgtcaa
     6301 aactgtgata aattatacac aactgaatct tatgaatatg ttgaaaatga aaaaaaagtt
     6361 atgtatagat gtaaagactc tgaaattggt tctgataaac atattttgaa tggatgtggg
     6421 catgaaggtg aggctgatat tacaaacggt cttggaaagt tagcatggaa agttgagttt
     6481 gctgcaagat gggaagcatt tgatataaga tttgaagcgt atgggaaaga cattatggat
     6541 tctgtaaaag tcaatgattg ggttgcagat gaagtgctag gatttccaca ccctcatcat
     6601 gtaaaatatg aaatgttctt agataaagga ggaaagaaaa tttcgaaatc acttggaaat
     6661 gttgtcacct cacaaaaatg gctgaactat ggaacacctg aatcaattct tcttttactt
     6721 tacaaaagaa ttactggtgc tagagaacta gggtttgaag atatacctgc attgatggat
     6781 gaatataatg aattggagaa tatctatttt ggaaaaacaa agatagaaaa tgaagcaaaa
     6841 cttgttaaat ctaaaggtct ttacgaatat gtaaatcttc taaatccccc aaaaaattct
     6901 gatgtgcata ttagttacgg attactaata gaattatgca agatattcaa agatgatcga
     6961 ttagaacgtg taaattcaaa attgattgat tatggtacaa taaaagaaac gaattcacgt
     7021 attgaggaat taatcaaact tgctggaaaa tattcggacg acttcgagga aacaaaaata
     7081 cctgctacta aaataataat tgatgattca tgtaagattg ccttgaccca attggttgaa
     7141 ttactattaa aaaatgagaa aaccgaagat ttacaaaatt caatatatac gatagcaaaa
     7201 gaaaaccaag tccaaccaaa agattttttc agaatacttt accaaatcat actttcagca
     7261 aatcgtggac caaaaattgg accgtttatt gaagacatcg ggaagaaaaa agttgcagac
     7321 aatatttcta agtatatttg atagtcatgg cacacaatga acataataag ccaaaaagaa
     7381 gtggttcttt cctattaatt atcgccggct ctatattgtt gatgctagca ccaactcaat
     7441 atccaacctc aaaggaattg ggaatacttg cattaatcag tggattaatt attggcagta
     7501 ttggattcta cataaaattt gtaaaaggga ggaaatctag aaagaataat gcttagagaa
     7561 aatgaatatg aattggttta gaaatacaaa gaaaaataca gataatgatt ctgaagattc
     7621 aaagaaaaat gatggtattg atattaaata taataaaaca gatgaaccac aaatagaaaa
     7681 taatacagtt aatgaacatg atacactttc aagcaaatta gaacttgttc aacaagagta
     7741 tcataaaatt gtgcatgaat taatgacatc aaaaaaagaa tttaataaaa tgaaagctga
     7801 catacaaaaa ttaaatgatg aaaatgatgt actaacatca aaaataaaat ctggtcacac
     7861 tgaactacta aaaataaata atgaattaaa agaaaaaaat gaaatattaa aaaaaataat
     7921 gaatgattac catagacaaa gtattgttat taaagaaact gacaaaacga aaaatgaact
     7981 agttcagatt aaagaagaga ttaaaaaata caacaatgaa ctagataatg caaaaacaaa
     8041 aacaaataat tctgttgaag ttcaaaaagc aagaaatgaa aaaaatgaat tggagagtga
     8101 gattctcaaa aaaagaaaag aagtagagtc tgccattaaa gaattaaagt ttattgaaag
     8161 tgagatgtca aaaataggta attataagca gactgaagtg attgctaata atcaaagtaa
     8221 cacaaacatt gttgacgctg ctagtgcagt agtgtcctca cttaataaaa aattgcaaat
     8281 tacacaaaaa gaattggaaa tggtaaagaa aaatcttgat atagtaagaa aacaaaaaaa
     8341 gaaaatacca taaaaaatta ctacgtccga ttaaagtgat agataatact ttttccattt
     8401 atcttttatt tcatcttctg tataaccgag atcataaagt ggtgaaataa cttcattcat
     8461 ttgttttact ttaactaaca caattgattc gataggattt ttcacaccag agatcttgta
     8521 atgtgaaata attttattgt atttagatcc agatgtgata atctctgcaa ttcccttgaa
     8581 tctgtaacct tttctgtaaa acggatcaat gatgttaatt tcaattgaat tattattttt
     8641 tagatttttc attgtttgtg gtgaccgtat attggcgaat gcaagagtgt ctttattcca
     8701 ggcaataatt gtgcccttcg gagacagatt tggcgtgcca tctgctgaaa cagttgcaac
     8761 aaatccaagt ttttgctcag acatgaattt tctgatttta tttgtgatca taattgattc
     8821 tggaggatga agtatattat taaatttgaa cgggcccgaa gggatttgaa cccccgacct
     8881 aatgctccgc aagcattcgc tctatccaag ctgagccacg agcccaataa cacgtttaga
     8941 aaaattgtta aaaacgtttt gaagtaatta tgcttcctgt tctgcctttt tcacaaagac
     9001 ataattcatt ataacacctg caataattcc gccaattatt ggtgctgccc agtaaagcca
     9061 ctgtatttct aacaaaccaa catcccctga tacaatagct ggtgcaaatg atcgtgcagg
     9121 attcatcgaa gccccagtta atgggacgcc tactaagtga agcaagaata ccattccacc
     9181 aattgctgca ccatagacgc tttttggtgc ttttttgtga accgcggtca tgaaaattac
     9241 aactaccaaa aagaatgtga gaataatttc taccatgaat ccagacattg cactattgtt
     9301 aagtaattca cttggaccac catgaccacc ccagagaact ggttttccaa tttctggtaa
     9361 tattgcaacc aaggttaacg ttgcaatgat gccgccaatt atttgagata taatgtatcc
     9421 cacaccatca gcgattccaa ttttctttgt gatcatcatt ggtatcgtaa cagctggatt
     9481 gatatgtgca ccagaaatat ggccaaaagc gtaaaccatg agtgcaatta ttccaccatg
     9541 tcccaaagaa atcataatta ttgcttcaat agataatcct tcaccaaaag caaccacagc
     9601 caaaattacg gataatggac caaagaacac caagccgaat gttgaaattc cttctgcaag
     9661 ccatgctctt ggatttacca tagatcacaa ccgatgatct tttccatata aaagattcga
     9721 attatttccc tgaacaaaaa gtaattaatt gggatattag aaaataggat atgatcgacg
     9781 aaggaaagac agtgcctatg tttgaattaa gtgatgcaga tggaaatatt gtaaagacaa
     9841 aggatttcaa ggggaaaaag tttgttgttt atttttatcc tagagatttt acacctggat
     9901 gtactgttga agcagatgaa ttttcgaaga attataagaa atttcagaaa ttaggaattg
     9961 atatcattgg tatcagtaaa gatgatgttg aatcacacaa aaaattttgt aagaaaatga
    10021 gtatacctta cattttgctt gctgatacca caaccaaagt ttctaaactg tttggagttt
    10081 ggggaaaaaa gaagtttatg ggaaaagagt atatgggtat taatcgtagc acatttttga
    10141 taaataaaaa gggaaaaatt ttcaaagttt ttgaaaaagt aaaaccaaaa ggtcattcaa
    10201 aagaagtgct agatgcatat gtcaatgcag aaaaataaaa ataaaaataa aaaattctag
    10261 aaacctttgg gtaatgcacc tttcttagat gttgttgcta ctgcttcttc tgctgcagct
    10321 gaaccctctt ctgcttgttg ttcaagtcta agcagataat ctttttcata atcttctaag
    10381 ctttgttgtt ttgatgtatt cccagaatca gcttgtggtt gactttctgg ttgtggtggt
    10441 ggtggtggtg ctgtttgtgt tgaatcacta ccagaaactg atgccgcaat tgattgctgg
    10501 aatgtcttgg tacgtggatg atatgacatt ctttgtctag ttgcaaaata cttgtcaccg
    10561 ggagcagtat aacctgtaac tactgctttt tgatcattcc aattctttgc tggaacatca
    10621 ggtgcatttt gtacatgtaa actagcccaa tatctatcac ttgcagtagt ataaccagtc
    10681 accttttctt tttgatcatt ccaatttttg gttggaactt gcttccactg ttcatataca
    10741 agagctggtg gtgccttttc tgctggcttt ggtggtggtg tttgtggttt tggtaatgtt
    10801 tgttgttttg gtggtggtgg tggtgcttgc gtttgtggtt ttggtggtgg tgcttgcgtt
    10861 tgtggttttg gtggtggtgc ttgcgtttgt ggttttggtt tagaaagatt acttagtttt
    10921 ctctcaagag cagcgatctt tgattcaaga gccttctttg aaggtgctgc ttttctagct
    10981 g
//
LOCUS       DASCPJ010000091        12668 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001490410, whole genome shotgun sequence.
ACCESSION   DASCPJ010000091 DASCPJ010000000
VERSION     DASCPJ010000091.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001490410"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="QGF01_04225"
     CDS             <1..350
                     /locus_tag="QGF01_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HJN20131.1"
                     /translation="SMIRVINEGINPVLVKSGYPELAVKIGVDFGKNMIVRYGSDKKT
                     SHVDLLGPSMNVASKIQNLAKPQQILIGEDVYKKIHPTLQKKCKQEILSEAKWKYRYR
                     NSDKLYPIYHYAD"
     gene            complement(347..496)
                     /locus_tag="QGF01_04230"
     CDS             complement(347..496)
                     /locus_tag="QGF01_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20132.1"
                     /translation="MIQRKKKTCLKCKKEIMMMELHKVVMYVIEDEFTDHHYEHVECP
                     DNFTI"
     gene            639..1001
                     /locus_tag="QGF01_04235"
     CDS             639..1001
                     /locus_tag="QGF01_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20133.1"
                     /translation="MVDFINMKKLNPYDFMHDFLAMPNSIRYPPGSNIPKGLINTSKL
                     KEKLSSDNAVLGPKLENFAEMYRQMAASLLNMSVDKLIPPGHPLYSKDHSLTLLKEEN
                     EKILKENLELKKKLENKK"
     gene            complement(1006..1410)
                     /locus_tag="QGF01_04240"
     CDS             complement(1006..1410)
                     /locus_tag="QGF01_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJN20134.1"
                     /translation="MNVRKVGNVILAVKDLEKSLEFYNKILGMPIKRQRENWVDLGQS
                     GALLSLHPASAELTTPNDMIMIGFVVGDVSSAIDELKSKNVKIHRDIQNKSSGKNAII
                     LDPDEYMISLFEPDFSAEKNIQSGGYIGFTPE"
     gene            complement(1445..1663)
                     /locus_tag="QGF01_04245"
     CDS             complement(1445..1663)
                     /locus_tag="QGF01_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HJN20135.1"
                     /translation="MVKAVVLVKSPKKLIAARLKKIDFISISFPVSGQFDAVAIIEVN
                     DLSQIKDVTTDIQKITGVIRTETMVQVK"
     gene            complement(1657..1896)
                     /locus_tag="QGF01_04250"
     CDS             complement(1657..1896)
                     /locus_tag="QGF01_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20136.1"
                     /translation="MYALCLITVNPGKIDKVISYLSKKRKPMKEVMTVTGRADVSILF
                     NGSLNDINNGVNELKRIKDIVSTETLIEVEVDLGW"
     gene            complement(1937..2152)
                     /locus_tag="QGF01_04255"
     CDS             complement(1937..2152)
                     /locus_tag="QGF01_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20137.1"
                     /translation="MVEKTTKVKKTSKKTSSTKIKKKSKAKSKNLDTYDDSGIVLIDD
                     DLEIDTEKVSEERKAYLEESRSQDASE"
     gene            complement(2223..2296)
                     /locus_tag="QGF01_04260"
     tRNA            complement(2223..2296)
                     /locus_tag="QGF01_04260"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2260..2262),aa:Phe,seq:gaa)
     gene            2566..3093
                     /locus_tag="QGF01_04265"
     CDS             2566..3093
                     /locus_tag="QGF01_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJN20138.1"
                     /translation="MILNFFQRVIELKTVPRQGWKNKLRLNDVESVAEHTYSTAILSM
                     IFSDLHGFDTEKIIKMALIHDLAESVIGDLTPNQIPKNKKNKLENATMKQILKNLPKK
                     ISNDYYHIWDEYQKKSSDESILLHEIDKLEMALQAKFYQDQGISKKDLRSFFATADAQ
                     ITNKHLRQLLRSFLE"
     gene            3180..3962
                     /locus_tag="QGF01_04270"
     CDS             3180..3962
                     /locus_tag="QGF01_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20139.1"
                     /translation="MVARRHKKKSAKHRNRGSGHDSIANKIIHEIINAKYDITENEAN
                     LVKDALNYSTETRTMTSLMKKDLESVTSSIFKSRRKLIEKKVSVDILKASIKEKKDIV
                     KKASSYVSHMKTKLTNEKIQMLNTQKGLLEITKAIDDITKVTSKLSDADADAATYGLK
                     ASLIAKEANSKKAEVQYNSTEKLANQAANRHDDAVKSLEKLERSFAHESSNLKIITDN
                     MKDAFDTLTNTYNDVKDLSLSLLDEAFVPEKSSQTTDTLAVQ"
     gene            4024..4371
                     /locus_tag="QGF01_04275"
     CDS             4024..4371
                     /locus_tag="QGF01_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20140.1"
                     /translation="MQYFLALKLGQKRVNTAREYLNKFANGKAMPALALKDNNTNIWE
                     PVGEENLYATVNGVDGFVVTDDTGYILVLCDKNGIAKTIAQGLSDEEKSTIINSLKLD
                     NIEEYHGKISLPV"
     gene            4429..5640
                     /locus_tag="QGF01_04280"
     CDS             4429..5640
                     /locus_tag="QGF01_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HJN20141.1"
                     /translation="MKKFSSEIELRGHIIDSLILTKVFDGIMDHGGSFEVLDIQVGKK
                     KKDESYAKLLVIGKNEKNLDRILNYAYRQGATSKTQSDVKLNAALKDMVMPDNFYSTT
                     NNQTQIFLKNKWVDVENMMMDKCIVVKAKKATCIPIRQIKKGDKIVVGENGIKVIPPE
                     RPREGMNVFEFMGSSSSSERPTQHIAKKVAEDIRLTKKNGGKIVLVGGPAIIHTGASE
                     SVSTLIRHGYIDAVLAGNALAVHDIEYATLGTSLGMNVRDGTLAVRGHRNHMEAINTV
                     FKAGSIEKMVKTKKLTRGIMYECVKKKIPFVLAGSLRDDGPLPDVITDVATAQKEYKR
                     VLKGASMVIMVATMLHSIATGNMLPANVKVIVIDINQPTVTKLMDRGTWQALGIVSDA
                     GAFLPMVAKEL"
     gene            complement(5801..7138)
                     /locus_tag="QGF01_04285"
     CDS             complement(5801..7138)
                     /locus_tag="QGF01_04285"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJN20142.1"
                     /translation="MTKKRILVLGGGFAGVDCTRKLENYFESNSDIEITLVSDDNFLL
                     FTPMLPQIVAGTVTPRHIVMPLRALCKKARIYESVVRDIDPIQQHVTLEGTPEKIGIR
                     IHYDYLVIALGSKTNFFGLNDVERNSFTMKTLADAISLRNRIIDMLEQAENEKNSKIK
                     ERLLTFVIVGGGFAGVETAGEINDFLNEAISYYHNIKLVDVQVILVEAVGTILQGFNE
                     SLSKYAHNKLEKNGIKILLQHAVTSFDGKNVTIKKLPSTSSNVKHEVTIDSHTLVWTA
                     GVTPVEIIQNSAFKLKNGKIIVNEFLEIPNHPEIFAIGDCSQFDREDVNKFPPTAQLA
                     EAHAKLAAYNIKQSIESKEKKKFVYKWKGQSAIIGKHYGIAESMGIKITGFWAWVLWR
                     NYYLNKMPNIEKRIRVWLDWNIDLFSRVDISRYGFKRDTSKEYRELDEVDDVW"
     gene            complement(7131..8540)
                     /gene="gatB"
                     /locus_tag="QGF01_04290"
     CDS             complement(7131..8540)
                     /gene="gatB"
                     /locus_tag="QGF01_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HJN20143.1"
                     /translation="MAKIGLEIHGQLTKLESKLFCPCKADYRQFEPNTNICPICMGIP
                     GALPLLNRTAVEKATLIAMALDCKTPNKIAFFRKNYFYPDLPKNFQITQLNVYGNTSI
                     GWEGKILVADKEIRIRRIQLEEDPGRLIYEGANERTKVTLVDYNRAGTPLVEIVTEPD
                     FETPHQVREFLNILSDLLENLNVSDPGLEGAMRADANVSIEGGSKVEIKNIGSFHDLE
                     KAVHFEITRQQSLAERKIEIVQETRHWDDGRKITVSARTKEEEAEYRYHLEAEIPWVR
                     INDDTKNQLRTHMPESISSKKKRYVSKYGITPQVADILSSDRYFSELFENAKNETNAK
                     DVANMITTDIMGLLDTREKREISKLSPEHLSDLVECIKSNKITRSSAKLALDEIIKNG
                     KSLEQIINDLDLGHVSDKEELSNIITQVLNEEAKAVEDAKLNPEIVNFLVGKVMQKTR
                     GKADPELTLSLLKKSLGID"
     gene            complement(8540..9988)
                     /gene="gatA"
                     /locus_tag="QGF01_04295"
     CDS             complement(8540..9988)
                     /gene="gatA"
                     /locus_tag="QGF01_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HJN20144.1"
                     /translation="MNLGISALNYVNSIKNGDFTVEEFIEKSFERIKKVDDKLHAFLS
                     FNDIALEKAKGIDRRISSNQKIGQCFGMPISVKDNMCVEGTKTTCASKVLENFISPYT
                     GTAVSRLETEDAIIIGKTNLDEFAMGLSTEFSAYGSSKNPWNVDYVPGGSSGGSAVSV
                     SANECIASLGSDTGGSIRNPASFCSIVGLKPTYGLVSRFGLVSYANSLEQIGPMTKTV
                     EDSAFLLNIISGIDHNDNTTIDNKNQDYLKDIDAGIAGKKIGVVTEMTNPNGLSTSVL
                     NATNDAIKKFEDLGANCEQVSLGMSKYAVAAYYTITSTEAGSNLARYDNIRYGYEFDS
                     SGFEFNSYISQARSKLGPEVIRRMILGGFVPSAGHAGKYFLKALKVKSKLITEINNIF
                     KKFDYLISPTVPIQPFKFGEKINDPVSFMLVDFNTVTANLTGIPAISVPFKIEDGLPI
                     GIQIMANHMDEHSLLQAAYALEKTVSLPEVEI"
     gene            complement(9989..10264)
                     /locus_tag="QGF01_04300"
     CDS             complement(9989..10264)
                     /locus_tag="QGF01_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20145.1"
                     /translation="MKKLVDEEEIIHVAKLMKIELADYNIHIKKVQKMLEYFDILDEA
                     NIESEDLIVQETDVDKLRDDKHVIYDKKLLKFLKNYQETYVKAPKLS"
     gene            complement(10265..11575)
                     /gene="aspS"
                     /locus_tag="QGF01_04305"
     CDS             complement(10265..11575)
                     /gene="aspS"
                     /locus_tag="QGF01_04305"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HJN20146.1"
                     /translation="MTESELDDWRRTHYSNELVSSLDELDVIVMGWISSVRGHGNISF
                     VTIEDMTGQIQIVSKKGVCPDQVLEEISKLKEHTCIGIKGRIKKSEKAPNGIEVIPND
                     LRIFSDVKIIPPFDPNAKTVKNIDTRLEIRAVDLRRNTLKKVFVARNEILKSIRNYFA
                     TQNFIEINTPKMIATATEGGAALFPIFYYNKEAFLAQSPQLYKEQLTMSFEKVFEIAP
                     IFRAEPSRTNRHLSEAISIDFEEAYIDYNDVMNRIEEIIKNSVKTMNDFAHKNPDTEF
                     LIPSISEKIPRYTYAELIDKMQKSGVKTQWGDDLYPSNLSKIGLSGFYFIIDWPLGPK
                     PFYVKATKNDPKISESFDLMYGDLEISSGSTRIQTKSELELRMKNKGMKVDSFSYHLK
                     IFDFGVPPHAGCGIGLERLMMALTGIENIRDTTFYPRDVDRLTP"
     gene            complement(11625..11966)
                     /locus_tag="QGF01_04310"
     CDS             complement(11625..11966)
                     /locus_tag="QGF01_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJN20147.1"
                     /translation="MQVIPQVVKIDNENRKQIYLDVLSDQFCLSILTSIINQPKTAVV
                     VAKETGIPISTVYRRLQFLQENKLLKVSGGINKDGKYFIYQSKIKEVSTKFDGISTMI
                     HVTPNLNFTIM"
     gene            12050..12259
                     /locus_tag="QGF01_04315"
     CDS             12050..12259
                     /locus_tag="QGF01_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20148.1"
                     /translation="MLNENDKIIAHVSSAIAVYSIRSSNGMLTNDISMIDFILKTTPK
                     NLEAKVSIDLIDDVFSYISGTHFDT"
     gene            12295..>12668
                     /locus_tag="QGF01_04320"
     CDS             12295..>12668
                     /locus_tag="QGF01_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1246 domain-containing protein"
                     /protein_id="HJN20149.1"
                     /translation="MATIVTLGSHCALQVLKGAKDEGFKTILVCEKKREKLYRRFKFI
                     DELIIVEKFSEILNQEIQDKLKQQDAILIPHGTLIAQMNSEEIESINVPIFGNKWILR
                     WESDREMKEKLMREATLPMPKPV"
BASE COUNT         4306 a         2096 c         1848 g         4418 t
ORIGIN      
        1 gatctatgat tagagtaatc aatgagggaa taaatcctgt tttagttaaa tctggctatc
       61 cagagcttgc ggtaaaaatt ggtgtagatt ttggcaaaaa tatgattgta agatatggat
      121 ccgataaaaa aacatctcat gttgatttat taggtccatc gatgaatgtt gcatcaaaaa
      181 tacaaaattt agctaaacca cagcaaattt taattggaga agatgtctat aaaaaaattc
      241 atcctacttt acaaaaaaaa tgcaaacaag aaatcttaag cgaggctaaa tggaagtatc
      301 gttatagaaa ttcagataaa ttatatccaa tttatcacta tgctgattaa atcgtaaaat
      361 tatcaggaca ttctacatgt tcataatgat gatcagtaaa ttcatcttca ataacataca
      421 taacaacttt gtgtaattcc atcatcatga tctctttctt acatttcaaa catgtctttt
      481 ttttcctctg aatcattttt tatcaaaaac atatgatcgc tataaggatc agcgatcaac
      541 tgtaattgat aaggccttaa tgagttgatc aagaattctt tactaagatt tttccaggca
      601 tactaaagca caaatatggc gataaaaata taaaaaatat ggttgatttt attaatatga
      661 aaaaactgaa tccttacgat tttatgcatg attttttggc tatgccaaat tctatacgat
      721 atccacctgg ctcaaatatt cctaaaggtt tgataaacac atcaaaactc aaggagaagc
      781 tttcttctga taatgcagta cttggtccta aattagaaaa ctttgccgaa atgtatcgtc
      841 aaatggcagc aagccttctt aacatgtcag ttgataaatt aattcctcca ggccatccat
      901 tatattcaaa ggatcactca cttacattac tgaaggaaga aaatgaaaaa attcttaaag
      961 aaaatttaga attaaaaaag aaattagaaa ataaaaaata aaattttatt caggagtaaa
     1021 tcctatgtaa ccgcctgact gtatgttttt ttcagcgcta aaatctggtt caaacaatga
     1081 aatcatatat tcatcaggat ctaatataat tgcatttttt ccagatgact tgttttgtat
     1141 atctctatga atttttacat ttttagactt caattcatca atcgcagatg aaacatcacc
     1201 tactacaaaa ccaatcataa tcatatcatt tggtgttgta agttctgcag atgctgggtg
     1261 aaggctaagt aatgccccag attggccaag gtcgacccaa ttctctcttt gtcttttgat
     1321 cggcatgcct aaaattttat tataaaattc gagtgatttt tctagatcct ttacagccaa
     1381 aataacattt ccaacctttc taacattcat atttttatca ctacataccg tcctaaatgc
     1441 tttattattt tacctgaacc attgtttcag tgcgtataac cccggttatt ttttggatat
     1501 ctgttgttac atctttgatt tgagacaaat cattaacttc tattattgct acagcatcaa
     1561 attgaccact aacaggaaat gatatggaga taaaatcaat ctttttgagt cttgccgcaa
     1621 tgagtttttt tggtgatttc actaatacta ctgcctttac catcctagat caacctcaac
     1681 ttctataagg gtttcagtag aaacaatgtc tttgattctt ttcaattcat tcactccatt
     1741 atttatgtca tttaatgaac cattaaaaag aatagaaaca tctgcccttc ctgtaacagt
     1801 cataacttct ttcatcggtt tacgtttttt tgaaagataa cttattactt tatcaatctt
     1861 acctggattg actgtgatta gacataatgc atacatgatg aatactatgt taaacatgca
     1921 taaaaattta gttaggctat tcagaagcat cctgtgaacg tgattcttcc aaatacgcct
     1981 ttctctcttc tgataccttc tctgtatcaa tttctaaatc atcatcaatc agaactatcc
     2041 cagaatcatc ataagtatcc aaattcttac tttttgcttt tgatttcttc tttattttag
     2101 tcgatgaagt ttttttactg gtttttttga cctttgtagt tttttcaacc atatattttc
     2161 agaaaatcag tatgaaatct tctattaaaa tattgccatt gcaaaataat ataaaatttt
     2221 aatgccagag gcgggatttg aacgcgcgac agctcggtct tcagccgagt gctctcccag
     2281 gctgagctac cctggcacaa cgcaattcaa attaataggg atttaaggtt gtcttttata
     2341 ctattatttg ttaattatta aatttgtgct tggaataact atcatattct aattttggat
     2401 tatatctgaa aaaataataa atttatgatt aaattaatgc aagcataatc caaattaata
     2461 ccaattctat gtttcatttt atgaaatata gaattaacat tcataaaaca atctaagatt
     2521 tgtaaaataa aaaaaattta tgagtgtgat caaaatagat actttatgat tttaaatttt
     2581 ttccaaagag ttatagaatt aaaaactgtt cctagacaag gttggaaaaa taaacttaga
     2641 ttaaatgatg ttgaatctgt tgcagaacat acttactcta cggcaatttt atctatgata
     2701 ttttctgatt tacacggatt tgatactgaa aaaatcatca agatggcatt gatacatgat
     2761 ttagcagaat cagtgatagg tgacttaaca cctaaccaaa taccaaaaaa taaaaaaaat
     2821 aaacttgaga atgctacaat gaaacaaata ttgaaaaacc tcccaaaaaa aatatcaaat
     2881 gattattatc atatttggga tgaatatcaa aaaaaatcct cagatgaatc catactacta
     2941 catgaaattg ataaactaga aatggcattg caggctaaat tctatcagga tcaaggtata
     3001 tcaaaaaaag atctgcgatc attttttgca accgctgatg cccaaataac aaataaacat
     3061 ctacggcaac tacttagatc atttttagaa taataagatg taactaaata ccaataaatt
     3121 ttctttttgt gaacaaagtt attttttaac tttaaaaata ggtctgatta tcatgagtta
     3181 tggtagcaag aaggcataaa aaaaaatcag caaaacatag aaatcgtgga tcaggtcatg
     3241 attcaattgc caataaaata atccatgaaa ttattaatgc taaatatgat attactgaaa
     3301 atgaggctaa tctagttaaa gatgccttaa actattctac tgaaacaaga actatgactt
     3361 cattgatgaa aaaagatttg gagtcagtaa ctagctcaat ttttaaatcc agaagaaaac
     3421 tgattgaaaa aaaagtatct gttgacatct taaaggcatc cataaaggaa aaaaaagata
     3481 ttgtgaaaaa agcatcatca tatgtgtctc atatgaaaac aaaattaacg aatgaaaaaa
     3541 ttcagatgct taatacacaa aaaggcctct tggagataac aaaagcaatt gatgatatca
     3601 caaaagtcac gtcaaaatta agtgatgcag atgcagatgc cgcaacatat ggactaaagg
     3661 cttcattaat tgctaaagag gcaaattcta aaaaagcaga agtgcagtac aattctacag
     3721 aaaaattagc taaccaagca gcaaatagac atgatgatgc agtaaaatca cttgaaaaat
     3781 tagaacgctc atttgctcat gaatcttcaa acctaaaaat aattactgat aatatgaaag
     3841 atgcatttga cacactaact aatacataca atgatgtgaa agatctttca ttaagtttgt
     3901 tggatgaagc atttgttcca gaaaaatcaa gtcagactac tgatactctt gctgtgcaat
     3961 aaataatttt ataaaaataa aaaaatcata aatgtattaa taataatcat tacaataaat
     4021 agtgtgcaat attttctagc tttaaaattg ggtcagaaac gtgttaatac tgcaagagaa
     4081 tatcttaata aatttgcaaa tggcaaagca atgccagcat tagcattgaa ggataataat
     4141 acaaatattt gggagccagt tggggaagaa aatttgtatg ctactgttaa tggtgtagat
     4201 ggatttgtag taaccgacga cactggatat attcttgttt tatgtgataa aaatggcata
     4261 gccaagacaa ttgctcaagg attatctgat gaggaaaaat ctactataat aaattcacta
     4321 aaattagata atattgaaga gtatcacggt aaaattagcc tcccagtata gtagatactc
     4381 aaattatttt gtcgtaacat tttacttacc aataattcat gtcaacgtat gaagaaattc
     4441 tcatcagaaa tagagctacg tggacatatc atagattccc ttatcctaac aaaagtattt
     4501 gatgggatta tggaccatgg tggttcgttt gaagttcttg acatccaggt tggtaaaaag
     4561 aaaaaagatg aaagttatgc aaaattactt gtaataggta aaaatgaaaa aaatcttgat
     4621 aggatattaa attatgcata tcgtcaagga gctacatcta agactcaaag tgatgtaaaa
     4681 ctaaatgcag cattaaaaga tatggtgatg cctgataatt tttacagtac aacaaataat
     4741 caaacacaga tttttcttaa gaataaatgg gttgacgtgg aaaatatgat gatggataag
     4801 tgcattgttg ttaaagcaaa aaaagcaact tgtattccaa tacgtcaaat aaaaaaggga
     4861 gataaaattg tagttggaga aaatggtatc aaagtaattc ctccagaaag acctagagaa
     4921 ggaatgaatg tatttgaatt tatgggaagt agtagttcaa gtgaaaggcc aacacagcat
     4981 attgctaaga aagttgcaga ggatatacga ctcactaaaa aaaatggtgg taagattgtt
     5041 ctggttggtg gtcctgctat tattcataca ggagctagcg aatcagtttc tactcttatt
     5101 cggcatggat atattgatgc agtcttggct ggaaatgccc ttgcagttca tgatattgaa
     5161 tatgcaacat tgggaacatc acttgggatg aatgttcgtg atggcacatt agcagtaaga
     5221 ggccatagaa atcatatgga agcgatcaat actgtattca aagctggctc tattgaaaaa
     5281 atggtaaaaa caaaaaaact cacacgtgga ataatgtatg agtgtgtaaa aaagaaaata
     5341 ccatttgttt tggcgggatc tttacgtgat gatggtccat tacctgatgt aattactgat
     5401 gttgcaactg ctcaaaaaga atacaaaagg gttctaaaag gtgcatcaat ggtaataatg
     5461 gtagctacaa tgttacattc aattgcaact ggcaatatgc ttcctgcaaa tgtcaaagtg
     5521 atagtgattg acataaatca accaactgtc acaaaattaa tggatcgtgg tacatggcag
     5581 gcattaggaa tcgtatctga tgctggtgca tttttaccaa tggtggctaa agaattataa
     5641 ttcaaataaa attagggctg caggaataag atttcgggat tatagaaaaa agacgcaata
     5701 aaaataccac ctaaaactcc agcaatgacg cctgatgcta aataaaaaaa tactgtattc
     5761 atatctgcgc taataataac tatctaataa ctaatatcaa ctaccaaaca tcatctacct
     5821 catctaattc acggtattct ttggatgtgt ctcgtttgaa gccatatcta gagatatcta
     5881 ctctagaaaa taaatcaata ttccaatcca gccaaactct tattcttttt tctatgttag
     5941 gcattttatt aagataataa tttctccata gcacccatgc ccaaaatcca gtaatcttta
     6001 tgcccattga ttcagctata ccataatgct ttccaattat ggcactctgt cctttccact
     6061 tgtatacaaa tttctttttt tctttgctct caattgattg tttaatatta tatgcagcta
     6121 attttgcatg tgcttctgct aactgtgcag ttggtggaaa tttgttaaca tcttccctat
     6181 caaattgtga acaatcacct attgcaaata tttctggatg atttggtatt tctaagaatt
     6241 catttacaat tattttccca ttttttagtt taaaggcaga attttgtatg atttcaactg
     6301 gagttactcc agctgtccaa accaatgtat gagaatcaat cgtaacttca tgttttacat
     6361 ttgatgaagt actaggcaat ttttttattg tgacattttt gccatcaaaa cttgtaactg
     6421 cgtgctgaag taatattttg ataccatttt tttctaattt attgtgtgca tattttgata
     6481 aactttcatt aaacccctgt aaaatagttc caacagcttc aactagtata acttgtacat
     6541 caaccagttt gatgttatga taataagaaa tagcttcatt aagaaaatcg ttaatttcac
     6601 ctgctgtttc tacaccagca aatccgccac caacaataac aaaggtaagt aatcgttctt
     6661 taatttttga atttttttca ttttctgcct gctctagcat atcaattatt ctgtttctta
     6721 atgatattgc atctgctaat gttttcattg taaatgaatt tctttcaaca tcatttaacc
     6781 caaaaaaatt ggttttgctt cccaatgcaa taaccaaata atcataatgt attctaatac
     6841 caattttttc tggtgttcct tctaatgtaa catgttgttg aataggatca atatctctaa
     6901 ctactgattc gtatattcgt gctttcttac ataacgctct aagcggcata acaatatgcc
     6961 taggagtgac tgttccagca actatttgtg gtaacattgg tgtaaaaagt aagaaattat
     7021 catcactgac cagtgttatt tcaatatcag aatttgattc aaaataattt tctaattttc
     7081 tggtgcaatc cactcccgca aaaccgccac ccaaaaccaa aattcgtttt ttagtcaata
     7141 ccaagacttt ttttcaatag tgataacgtt aattctggat ctgccttacc tcgtgttttc
     7201 tgcataactt tgcctaccaa aaaattaact atttcaggat ttagttttgc atcttccact
     7261 gcttttgctt cttcatttaa tacttgtgta ataatattag ataattcttc tttgtctgaa
     7321 acatgtccta aatcaagatc gttaattatt tgttctaatg attttccatt ctttatgatt
     7381 tcatctagtg caagttttgc agaagacctt gtaattttat ttgatttgat acactcaact
     7441 agatctgaaa gatgttctgg agatagtttt gatatttcac gtttttcacg tgtatcaagt
     7501 aatcccataa tatcggtagt aatcatattt gcaacatctt ttgcatttgt ttcattcttt
     7561 gcgttttcaa aaagttctga aaagtatcta tctgaagata atatatctgc aacctgtggc
     7621 gtaataccat actttgaaac atatcgcttc ttctttgaac tgatactttc aggcatgtgt
     7681 gttcgtaatt gattttttgt atcatcatta attctcaccc acggtatctc tgcttcaaga
     7741 tggtatctat actcggcttc ttcctctttt gttcttgctg aaacagttat ttttcgacca
     7801 tcgtcccaat gtcttgtttc ttgaacaatc tcgatttttc gttctgcaag actttgttgt
     7861 cttgttattt caaaatgaac agccttttct aaatcatgaa atgagccaat attttttatt
     7921 tcaacctttg aaccgccttc tattgaaaca ttagcatcgg cacgcattgc accttctaga
     7981 cctggatctg atacattcaa attttcaagt aaatctgata gtatgtttaa aaattctcta
     8041 acttggtgtg gggtttcaaa atctggctcg gttacaattt caaccaatgg tgttccagcc
     8101 ctattgtaat caactaacgt aacttttgtt ctttcatttg caccttcata aattaatcgt
     8161 cctggatcct cttctaattg tattcttcga attcttatct ccttatctgc cactaagatt
     8221 ttaccttccc aaccaatact tgtatttcca tatacattga gctgagtgat ttgaaagttt
     8281 tttggcaaat caggatagaa ataatttttc ctaaaaaatg caattttatt aggagtctta
     8341 caatcaagtg ccattgcaat taatgttgct ttttcaacag cagttctatt cagtaatggt
     8401 aatgctcctg gaattcccat acagattgga cagatgtttg tatttggttc aaactgacga
     8461 taatctgctt tacatggaca aaatagttta ctttcaagtt ttgttaattg accatgtatc
     8521 tctagaccga tttttgccat cagatttcta cctcaggtaa acttactgtt ttttctaatg
     8581 catacgctgc ttgtaataat gaatgttcat ccatatgatt tgccattatt tggattccta
     8641 ttggtaagcc atcttcaatt ttaaatggta cagatattgc aggaattcca gtcaagtttg
     8701 cagtaacagt gttaaaatca actaacataa acgatactgg atcgtttatt ttttcaccga
     8761 atttgaacgg ttgaatggga actgttggtg aaattaaata atcaaatttt ttaaatatat
     8821 tatttatttc tgtgatgagt ttagatttta cttttaatgc cttcaaaaaa tattttccag
     8881 catgccctgc tgatggtaca aaaccaccta gaatcattct tctaataact tcagggccca
     8941 gtttacttct tgcttgtgat atatacgaat taaactcgaa gcctgatgag tcaaattcgt
     9001 atccatatct tatattatca tatcttgcaa gattacttcc tgcttctgtt gatgttatag
     9061 tataatatgc agctacagca tattttgaca ttcctaatga cacctgctca caatttgctc
     9121 caagatcttc aaattttttg attgcgtcat tggtagcatt tagaacacta gtactcaatc
     9181 cattaggatt tgtcatttct gtaactaccc caattttttt cccagcaata ccagcatcaa
     9241 tatcttttag ataatcctga tttttattat caatagtcgt attatcgtta tgatcaattc
     9301 cagaaattat attcaataaa aatgcagagt cctctactgt ttttgtcatt ggacctatct
     9361 gttcaagact atttgcgtaa gaaacaagac caaaacggct aacaagtcca tatgtaggtt
     9421 ttaaaccaac aattgaacaa aaacttgcag gatttctaat tgatccacca gtatcagatc
     9481 ccaacgatgc aatacattca ttcgcagaaa cagatacagc actgccacct gatgaccccc
     9541 ctggaacata atcaacattc caaggatttt tactggatcc atatgcacta aattctgtac
     9601 taagacccat ggcaaattca tcaagatttg ttttaccaat tattattgca tcttctgttt
     9661 ctaatctaga aactgcagtg ccagtataag gtgaaataaa attttctaaa acttttgatg
     9721 cgcatgtagt ttttgttcct tctacacaca tattgtcttt aacagaaatt ggcataccaa
     9781 aacactgtcc aattttttga tttgaactta ttcgacgatc aattccctta gccttttcta
     9841 atgcaatgtc attaaatgat aaaaaagcat gtagtttatc atctactttc ttgatccttt
     9901 caaatgattt ttcaataaat tcttctactg taaaatcccc gttcttgata ctatttacat
     9961 aatttaatgc agaaattcct aagttcattc aactcaactt tggagccttt acatacgttt
    10021 cctgataatt ttttagaaat tttaataatt ttttatcata gatgacatgt ttatcatctc
    10081 ttaatttatc aacatcagtt tcctgtacga ttagatcttc agattcaata tttgcctcat
    10141 caagaatatc aaaatattca agcatttttt gaactttttt tatatgtatg ttataatctg
    10201 caagttcaat tttcatcaat ttagcaacgt gtataatttc ttcttcatca accaattttt
    10261 tcacctatgg tgttaatcta tcaacatctc ttggataaaa tgttgtatct ctgatatttt
    10321 cgatcccagt taatgccatc atcagtcttt caagacctat tccacatcca gcatgtggtg
    10381 gaacaccaaa atcaaatatt tttagatgat aactaaatga atctactttc atgcctttat
    10441 ttttcattcg taattcgagt tcagatttgg tctgaatcct agtacttcct gatgatattt
    10501 ctaaatcacc gtacattaag tcaaaagatt ctgaaatttt tggatcgttt ttggttgctt
    10561 ttacataaaa tggttttgga cctaatggcc aatcaataat aaaatagaat ccagataacc
    10621 caattttgct caaattggat ggatatagat catcacccca ttgtgttttt actccagatt
    10681 tctgcatttt atcaatcaat tcagcgtatg tgtacctagg aattttttca gatatacttg
    10741 gaattaaaaa ctcagtatca ggatttttgt gtgcaaaatc attcatggtc ttaacagaat
    10801 tttttataat ttcttctatc ctattcatta catcattata atcaatgtac gcttcttcaa
    10861 agtcaatcga tatagcttct gatagatgtc tatttgttct tgatggttcc gcacgaaaaa
    10921 ttggagcaat ctcgaatact ttctcaaaac tcattgtgag ttgttcttta tataattggg
    10981 gactctgtgc aagaaatgcc tctttgttat aataaaatat aggaaaaagc gcagcaccac
    11041 cttcagttgc agttgcaatc atttttggtg tgttaatttc aataaaattt tgtgttgcaa
    11101 aataatttct tattgatttt aatatttcat tacgtgctac aaaaactttc tttaacgtat
    11161 tacgtcgtag atcaactgct ctaatttcta atcgtgtatc aatattttta acagttttag
    11221 cattaggatc aaatggagga attattttca cgtcagaaaa gattctaaga tcatttggga
    11281 taacttctat accatttgga gctttttcag atttttttat tcttcccttt ataccaatgc
    11341 aagtatgctc ttttaattta gaaatttctt ctaatacctg atctggacaa acaccttttt
    11401 tagaaactat ttgaatttga cctgtcatat cttctatagt aacaaatgag atatttccat
    11461 gacctcttac actagatatc catcccataa ctataacatc caattcgtcc aaagatgaaa
    11521 ctaattcatt tgaataatgt gttcttctcc aatcatccaa ttctgattct gtcattatat
    11581 gattttttat aatcgagtat ttattaggat tattttagta attatcacat tattgtaaaa
    11641 ttaaggttag gtgtgacatg aatcatagta gatataccgt caaattttgt tgaaacttcc
    11701 tttatcttac tttgataaat gaaatatttc ccatctttgt taattccacc tgaaactttc
    11761 aatagtttat tctcttgaag gaattgaagt cttctatata ctgtagatat aggtatgcca
    11821 gtttctttag caaccacaac agcagttttt ggttgattga ttattgatgt tagtattgat
    11881 aaacaaaatt gatcagataa tacatcaagg taaatttgtt tacgattttc attatcaatt
    11941 ttaacaactt gaggaattac ttgcaatagt aatttgatga atataccact ataaattaga
    12001 ttcttttcaa atagtattta taaaaataat tagtaaaaag taattaaata tgcttaatga
    12061 gaatgacaaa attattgcac atgtatctag tgcaattgca gtttattcta tacgaagtag
    12121 taatggaatg ttaacaaatg atatctctat gattgatttt atactaaaaa caacaccaaa
    12181 aaatttagag gcaaaagtat ctattgactt aattgatgat gttttttcat atatatcagg
    12241 cacacacttt gatacatagt ttcatatgtt gtgtttatct catgaaaata tagtttggca
    12301 acaatagtaa ccttaggttc acactgcgcg cttcaagttt taaaaggtgc aaaagatgaa
    12361 ggatttaaaa cgattcttgt ttgtgagaaa aaacgtgaaa aattatacag gagattcaaa
    12421 tttattgatg aattaatcat tgttgaaaaa ttttccgaga tattgaatca agaaatacaa
    12481 gataaactca aacagcaaga tgcaatatta attcctcatg gaacattaat cgcacaaatg
    12541 aactctgaag agatcgaatc tattaacgta ccaatttttg gaaacaaatg gattcttaga
    12601 tgggaatcag acagagaaat gaaagaaaaa ctcatgagag aagcaacact tccaatgcca
    12661 aaaccagt
//
LOCUS       DASCPJ010000092         7680 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001490943, whole genome shotgun sequence.
ACCESSION   DASCPJ010000092 DASCPJ010000000
VERSION     DASCPJ010000092.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001490943"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            75..443
                     /locus_tag="QGF01_04325"
     CDS             75..443
                     /locus_tag="QGF01_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HJN20150.1"
                     /translation="MAHIYSSYNNTIIHITDLTGAETVSISSGGHHVNAARYESSPFA
                     AMKAAKAVVDAAKTKGFTGLHIKVRAVGGVGSRVPGPGAQAAIRALARGGFKIGRIDD
                     VTPIPHDTTRRKGGKRGRRV"
     gene            complement(440..751)
                     /locus_tag="QGF01_04330"
     CDS             complement(440..751)
                     /locus_tag="QGF01_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20151.1"
                     /translation="MPTFEISTTTFDEDISVIQVALTKIAAICDFESEFKIAEPVSRF
                     GWTFFQILIQQELYFGISDKFSDMIKKCKGDKVNEKFVNFLIRYFDSCGCKVRIKWIS
                     D"
     gene            complement(752..1174)
                     /locus_tag="QGF01_04335"
     CDS             complement(752..1174)
                     /locus_tag="QGF01_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HJN20152.1"
                     /translation="MTEEDKDLEMLKAKRLAEMQKNISSQQKQEDSNVPQGEQKKNEP
                     TPREIVVQQLGYRGLEVLGNAEYQFPKETQIIILKLAELISSGSVTETLEGGKLLALF
                     RGVGINVRMKNKINVEKDGKFVSLSDKLRIKESSVKDD"
     gene            complement(1171..2619)
                     /locus_tag="QGF01_04340"
     CDS             complement(1171..2619)
                     /locus_tag="QGF01_04340"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HJN20153.1"
                     /translation="MSNFRPKKIGNMRYQIDADSSKGMKVPVTIYADEKLLAKMMTDR
                     TIQQAVNVSTLPGIQEHAIVLPDGHEGYGFPVGGVAAMDAEEGMISPGGVGYDINCGV
                     RLLRTNLTEGDVRPKLKDLVNDLFKSIPSGVGSKGAIRLNPSELDEVLVRGVTWAIDH
                     GYGISDDANVCEESGQIANADPNKVSERARKRGAPQLGSLGSGNHFLEVQKVAEIHDE
                     KAAKAMGIEKDSVTILIHCGSRGFGHQICSDYLRISEQAQKKYNIQLADRELACVPNK
                     SEEGESYRAAMFAALNFAWSNRQMISHWTRKSFERVFKQSESDLDMKLVYDVAHNIAK
                     VEKHKIAGKLKSVVVHRKGATRAFPANMEDVPSKYRDLGQPVLVPGSMGTGSWILLGQ
                     ENSMNTSFGSTAHGAGRMMSRSKARRDFSESEVKKSLNDKGIFLKSLTRDGVVEETPQ
                     AYKDVDAVVNVSHELGIATKVAKLVPIGVIKG"
     gene            2691..3338
                     /locus_tag="QGF01_04345"
     CDS             2691..3338
                     /locus_tag="QGF01_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJN20154.1"
                     /translation="MKTAIVLGGSRGIGKAIAESLKSIGCDVIATSKNELDTSSLESV
                     SNFAEKHNEVDILVLNTGGPEPKEFFSVTQDDWKHYHNQLFLGFCLLLQKIKINDNGY
                     VFLMSSNVIKEPNPKLIISSAYRSAFSSVFKILSKEFAKKQISCINIAPGPIQTDRTK
                     ELIDDVEAFAETLPMKRLGKPEEIGNFVRSIVEHDIKYLSGAVINFDGANSNFVY"
     gene            3379..4740
                     /gene="purB"
                     /locus_tag="QGF01_04350"
     CDS             3379..4740
                     /gene="purB"
                     /locus_tag="QGF01_04350"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HJN20155.1"
                     /translation="MSILPIDTGRYGTKEMLEIFKEQKKIDYQLDIEAAAALSQSEIG
                     LIPASVAKDISRIAKSGKITAKRIKQLESKSDHDTAALVEALAEKCKTESRPWIHYGL
                     TSNDLVDTSNSMQMRDAIKIIQPKVSKLSLILAKNAVKHKSVPAVGRTHGQHASIISF
                     GLKFANWASEMSTHLERLDEIKKRILICKTLGVVGTGSLMGKNAIRVQERVARKLSLY
                     PAKVTTQIIPRERYAEYIFQLALLGATLEKIAIEIRNLQRTEIAEVSEHFKKGQMGSS
                     AVPTKRNPIKSERISSLSKLLRSQISTSFENIPLWHERDLSNSANERFILPMTSILLD
                     EMLETMTKIIGNLQVDTKRVQENLHITKGQIFAEFVLEALIKKGIPRIKAYRDVQRVA
                     FAAHKKGINFMYALENDKAVSSILSDKEIKSVFVAKNHLGASLSIIDNVERHVKVNSK
                     KFS"
     gene            complement(4724..5479)
                     /locus_tag="QGF01_04355"
     CDS             complement(4724..5479)
                     /locus_tag="QGF01_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HJN20156.1"
                     /translation="MNAQLLTEMQNAVKGTVTEKRIGVAFSGGVDSTLLAKLVKDMGY
                     ETHLLTIGFHDSHDINFAKEVNEILNLPHSISEIDPEKFKEITDKIHQLIKSDNLSWN
                     ENSIAFYYVAELAQKNGLSMVVTANGIDELFCGYNSYREAIETGEDEVEKMMNNKLKN
                     EKEMMIAVNAVTAEFGVRMIQPFLSPNFIDHAKKIPISEKIHGSDDMKRKHPVRELAM
                     DYGVPEIAAQKQKKALQYGTQIHKSLLKARKTS"
     gene            5534..5815
                     /locus_tag="QGF01_04360"
     CDS             5534..5815
                     /locus_tag="QGF01_04360"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HJN20157.1"
                     /translation="MGLTKLSDEQIQTALQGLEGWSVVEGKLHKEFQFSDFNEAFGFM
                     ARASMHIEKMNHHPEWFNVYNKLVVDLMTHDASGITENDTKLASILNSL"
     gene            5818..>7680
                     /locus_tag="QGF01_04365"
     CDS             5818..>7680
                     /locus_tag="QGF01_04365"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HJN20158.1"
                     /translation="MDPKIQEKVWDPKIESEIRKKWENSKLYEFQADADGYTIDTPPP
                     YPSGRPWHIGAAAHYSQIDMISRTARMNGKNVYFPIGIDRNGLPVELYTEKKHGIRMR
                     ETERGKFLDLCKDALDDLEAEMIQIMKSLGLSCDFDNYYRTDSEEYRALTQSTFIELW
                     KNDSIYLATRPNNYDWVTGTTIADAEIIYEELPTKLVHMKFKIADGNEVIIASTRPEL
                     LCACKTIIVNPEDERYSDLIGKKVTVPISNQEVEIQTHHSAQMEFGSGAVMVCSYGDQ
                     NDVALFRELSLEEVVAIGQNGRLTEAAGDYAGLKPKQARTKIIEDLESGGFVEKIEDI
                     SHRTPISERSKTPIEIIPMEEYYLKQKESVGKMRELGAQIKFYPEMHQQILMNWLDSI
                     KIDWPISRRRFYGTEIPIWYCKKCSEPYLPEPGKYYRPWNEPCPAEKCAKCDSTEFVG
                     EDRTFDTWMDSSISPLFITKFKKDEEFFKRTYPTGVRPQAKDIVRTWLYYTLLRCENL
                     TGEKPWSEAWVMGYGLDEEGKKMSKSKGNALDPIPIIEKFGADTFRFWSASEVNHGSD
                     FRCSEQKIESTKKFLTKLWNVSRFLSSFPVIESAEISDTDKWIISELDKLVKDCK"
BASE COUNT         2510 a         1423 c         1405 g         2342 t
ORIGIN      
        1 aagtaaagac tgaagctgaa gtaaaaactg aagtacagcc tgaagtcaaa actgaaggta
       61 aagaaaaatg gggaatagcg catatttaca gtagttataa taatacaatc atccacatta
      121 ctgatcttac tggagctgaa acagtttcaa ttagctcagg tggtcatcat gtcaatgctg
      181 caagatacga gtcttcacca tttgctgcaa tgaaagctgc taaggctgtg gttgatgctg
      241 ctaagacaaa aggttttact ggattacata tcaaagtaag ggcagttgga ggtgttggct
      301 caagagttcc tggacctggt gctcaagcag caattagagc acttgcccga ggtggtttca
      361 aaattggacg tattgatgat gtcacgccta ttccacatga tactactaga aggaaaggtg
      421 gtaagcgtgg aagaagagtt taatctgata tccatttaat tctaacttta caaccgcatg
      481 aatcaaaata acgaatcaaa aagtttacga atttttcgtt cactttatcc cccttacatt
      541 ttttgatcat atctgaaaat ttatcactga ttccaaaata caattcttgt tgtattaata
      601 tttgaaaaaa tgtccatcca aatcttgaaa ctggttctgc aatcttaaac tccgattcaa
      661 aatcacaaat agcggcaatt ttagtcagag ctacttgaat tactgatata tcttcatcga
      721 atgttgttgt acttatttca aatgtaggca tctaatcatc ttttactgaa gattctttga
      781 tcctaagttt atcagaaagt gaaacaaatt ttccatcttt ctctacatta attttgtttt
      841 tcattctgac gttaatccca acacctctaa ataaagccaa taacttacca ccctcaagtg
      901 tttctgtaac acttcctgat gaaattaatt cagctagttt tagtataatt atctgtgttt
      961 cctttgggaa ttgatattct gcattaccta aaacttctaa tcctctatag cctaattgtt
     1021 gaacaacaat ctcgcgtgga gtaggctcat tttttttctg ttccccttgt ggcacgttag
     1081 aatcttcttg tttctgttga gaggaaatat ttttttgcat ttcagccaag cgtttagcct
     1141 tgagcatttc tagatcttta tcttcttcag tcatcctttg ataacaccta ttggtactaa
     1201 ttttgctact tttgtagcaa ttccaagttc atgtgacaca ttaactaccg catcaacgtc
     1261 tttgtaagct tgtggtgttt cttctaccac tccatcacgt gttaatgatt ttagaaaaat
     1321 tcctttatca ttaagagatt ttttaacctc agattcacta aagtctcgcc ttgcttttga
     1381 gcgggacatc attcttcctg caccgtgtgc tgtggaacca aaacttgtat tcatagaatt
     1441 ttcttgtcct aaaagtatcc aacttcctgt tcccattgag cctggtacta aaactggttg
     1501 accgagatca cgatactttg agggcacatc ttccatattt gcaggaaatg ctctggtagc
     1561 tccttttcta tgaaccacaa ctgattttaa ttttccagcg attttatgtt tttccacttt
     1621 tgctatgttg tgtgccacat catagactag tttcatatcc agatcggatt ctgattgttt
     1681 gaaaaccctt tcaaaagatt ttcttgtcca gtgggaaatc atttgtctat tactccaggc
     1741 aaaatttagt gctgcaaaca ttgcagctct atacgactct ccctcttcag atttattggg
     1801 aacacacgca agttctctat ctgccaattg aatattgtat tttttttgtg cctgttcaga
     1861 aattctaaga taatcactac atatttgatg accaaatcct cgtgaaccac aatgaatcaa
     1921 tatggtaaca ctatctttct caattcccat tgcctttgct gccttttcat catgaatctc
     1981 tgcaactttt tgtacttcaa gaaaatgatt tccggaacca agacttccta actgtggtgc
     2041 acctcttttt ctagcacgct cagatacctt gttaggatca gcatttgcta tttgcccact
     2101 ttcttcacat acatttgcat catcgcttat accataccca tgatcaattg cccaagttac
     2161 tcctctaacc aaaacttcat ctagttctga aggatttagt cggatagctc ctttagaacc
     2221 aacacctgaa ggaattgatt tgaaaagatc atttacaaga tcttttagtt tgggtctaac
     2281 atcaccttct gttaaattag ttctaagaag tctaacacca caattgatat catatcctac
     2341 acctccaggg ctaatcattc cttcttctgc atccatagca gcaactcctc caacaggaaa
     2401 accatatcct tcatgaccgt cgggtaaaac tattgcatgt tcttgaattc caggaagtgt
     2461 ggatacattc acagcttgtt gaattgtcct atcagtcatc attttggcca ataatttttc
     2521 atccgcataa attgttacag gtactttcat tcctttagaa gaatctgcat ctatctgata
     2581 tctcatattt ccaatttttt taggtctgaa attagacatg tagttttgaa ttattgtttg
     2641 acaatttaaa tcataattag attaaaatga aatgagtttg taagacattt atgaaaactg
     2701 ccatagtttt aggaggatca agaggaattg gtaaggcaat tgctgaatct ctgaaatcaa
     2761 taggctgtga cgtaatagct acctcaaaga acgaattgga tacatcaagt ttagaaagtg
     2821 tttcaaattt tgctgaaaaa cataatgaag ttgatatctt ggtactaaac acgggtggac
     2881 ctgagccaaa agaatttttt tctgtaaccc aagatgattg gaaacattac cataaccaat
     2941 tgtttctagg cttttgttta cttttgcaaa agatcaaaat caatgacaat ggatacgtgt
     3001 ttctcatgtc ttctaatgtg ataaaagaac ccaacccaaa attaatcatt tcaagtgctt
     3061 atcgctccgc tttctctagt gtctttaaaa ttcttagcaa agagtttgca aaaaaacaaa
     3121 taagttgtat caatatagca ccaggaccaa tccaaactga tagaacaaaa gaactaattg
     3181 atgatgttga agcatttgct gaaactctcc caatgaaaag acttggaaag ccagaagaaa
     3241 ttggcaattt tgtaagatca atagttgaac atgatatcaa atatctttct ggtgcagtaa
     3301 tcaattttga tggcgcaaac tcgaattttg tttactagta agacatagat ttatttctag
     3361 aataaactgg aaataattgt gtccatttta cctatagata ctggccgtta tggtacaaag
     3421 gaaatgctgg aaatttttaa ggaacaaaaa aagattgatt accaattaga cattgaagct
     3481 gcagctgcat tatcacaaag cgaaatagga ttgattcctg cttctgttgc aaaagatata
     3541 tccagaatag caaaatctgg aaaaattact gccaaaagaa tcaaacagtt agaatctaaa
     3601 agtgatcacg atactgctgc attggtagaa gcactagcgg aaaaatgtaa gacagaatca
     3661 agaccgtgga tacattatgg gttaacatcg aacgatctag tcgatacaag taactcgatg
     3721 caaatgagag atgccattaa aattattcag ccaaaggttt caaaactatc gttaattctt
     3781 gcaaaaaatg ctgtaaaaca taagagtgtt ccagctgttg gaagaacaca tgggcaacat
     3841 gcaagtatta tttcgtttgg attaaagttc gcgaactggg cttcggaaat gtcaacccat
     3901 cttgaaagat tggatgagat aaagaaacga attcttatct gtaagacact tggggtggtt
     3961 ggaacaggat ccctaatggg taaaaatgca ataagagttc aagaacgtgt agctagaaag
     4021 ctttcacttt atccagcaaa ggtcactaca caaattattc ctagagaaag atatgctgaa
     4081 tatatttttc aattagcgtt gcttggagca actttggaaa aaatcgctat agagattaga
     4141 aacttacaac gaacagaaat tgctgaggtt tcggaacatt tcaagaaggg tcagatggga
     4201 agcagtgcag tacccacgaa aagaaatcca ataaaaagtg aaagaatttc atccttatca
     4261 aaacttctaa gaagtcaaat cagtacatcc tttgagaaca ttccgctctg gcatgaaaga
     4321 gatttgtcaa attctgcaaa tgaacgattc attctaccaa tgacatctat tctactggat
     4381 gaaatgctag aaactatgac caaaataatt ggaaaccttc aggttgatac aaagcgtgta
     4441 caagaaaatc ttcacataac aaagggacaa atctttgctg aattcgtgtt agaagcatta
     4501 atcaaaaaag gtataccacg aatcaaggcg tatagagatg tgcaaagggt tgcatttgcc
     4561 gcacataaga aaggaatcaa ctttatgtat gcattagaaa atgataaggc tgtttcatct
     4621 attctttcag ataaggaaat aaaatcagtc tttgttgcaa aaaaccatct tggagcatca
     4681 ctatcaataa ttgataatgt agagagacat gttaaagtaa attctaagaa gttttcctag
     4741 cttttaacaa agatttgtgt atttgagtac catactgtaa tgcttttttc tgtttttgtg
     4801 cagcaatttc tggtacacca taatccatag ccaactcacg aacaggatgt tttcgcttca
     4861 tatcatctga accgtgaatt ttttcagaga tgggaatttt ctttgcatga tctataaaat
     4921 ttggtgatag aaatggttgg atcattctta caccaaattc tgcagtaact gcattgactg
     4981 caatcatcat ttctttttca ttttttaatt tattattcat cattttctca acttcatctt
     5041 cacctgtttc aattgcctca cggtatgaat tataaccaca aaataattca tctataccat
     5101 tagcagtaac aaccatactc aatccattct tctgtgcaag ttctgcaaca tagtaaaatg
     5161 caatagaatt ttcattccat gagagattat ccgacttgat taattgatga attttatcag
     5221 tgatctcctt gaatttttca ggatcaatct ctgaaatact gtgtggcaaa tttagtattt
     5281 catttacttc cttggcaaaa ttgatgtcgt gtgaatcatg aaacccaatt gttagcagat
     5341 gtgtctcata ccccatatcc ttgacaagtt ttgccagcaa agtactgtca acacctcctg
     5401 aaaatgcaac tcctattctc ttctctgtga cagtgccttt taccgcattc tgcatttctg
     5461 taagtaattg cgcattcatg attctaatta tataatctag ataatttatt aattaaccat
     5521 acaatgcttc tgcatgggat tgactaaact ttcagatgag cagatacaaa cagcacttca
     5581 aggtttggaa ggatggtcag tcgtagaggg taaactccat aaagaattcc aattttctga
     5641 tttcaacgag gcatttggct tcatggctag ggcatctatg catattgaaa agatgaacca
     5701 ccatccggaa tggttcaacg tatacaacaa gctagttgtt gacctgatga cgcatgacgc
     5761 ttcaggtatt actgaaaatg ataccaaatt ggcaagtatc cttaattcac tttagatatg
     5821 gacccaaaaa tccaagaaaa agtatgggat cccaagattg aatcagagat tagaaaaaaa
     5881 tgggagaatt caaaacttta cgaatttcaa gcagatgctg acggttatac gatagatact
     5941 cctccaccat atccttcagg tagaccatgg cacattggtg ctgcagcaca ttattctcaa
     6001 attgatatga tttcgagaac agcacgtatg aacggaaaga atgtttactt tccaattgga
     6061 atcgatagaa acggtttacc agtagagctc tacactgaaa aaaaacatgg cataagaatg
     6121 cgagaaacgg aaagaggaaa gtttcttgac ctgtgcaagg atgcactcga tgatcttgaa
     6181 gcagaaatga tacagatcat gaaaagcttg ggactcagtt gtgactttga taactattac
     6241 agaaccgact cggaagaata ccgagcgtta acacaatcta cattcataga actgtggaag
     6301 aatgattcaa tttacctagc aacgagacca aacaactatg attgggtaac tggaacaaca
     6361 atagcagacg ccgagataat ctacgaagaa cttccaacca aacttgtcca catgaaattc
     6421 aagatagcag atggaaatga ggttatcatt gcaagtacac gacctgaact tctatgtgct
     6481 tgtaaaacta taattgtaaa cccagaagac gaaagatatt ctgatctgat tggaaaaaaa
     6541 gtaactgtcc caatatcaaa ccaagaagtt gagatacaaa cacatcactc ggcacaaatg
     6601 gagtttggtt ccggtgcggt gatggtctgt agctatggtg accagaacga tgttgcgtta
     6661 tttcgagaac taagtctgga agaagttgtt gcaattggac agaatggaag actgaccgag
     6721 gctgctggcg attatgctgg tctcaaacca aaacaagctc gtacaaaaat tattgaggat
     6781 ttggaaagtg gtggatttgt tgaaaagatt gaagacatct ctcataggac accaatatcc
     6841 gagagaagca agactccaat cgagatcatt ccaatggagg aatattacct caagcaaaaa
     6901 gaatcagttg gaaaaatgag agaacttggg gctcaaatca agttttaccc agagatgcac
     6961 cagcaaattc tgatgaactg gcttgactca atcaagatag actggccaat ctctcgtagg
     7021 cggttttacg gaacagaaat tccaatctgg tactgtaaaa aatgctcaga gccatacttg
     7081 cctgagcctg gaaaatatta tcgaccatgg aatgagccat gtccggcaga aaaatgtgcc
     7141 aaatgtgact ctaccgagtt tgtgggtgag gatagaacgt ttgatacgtg gatggactct
     7201 agtatttcac cattattcat tacaaaattc aaaaaggatg aggagttttt caagagaact
     7261 tatccaactg gagtgagacc acaggcaaaa gacattgtca gaacgtggct gtactataca
     7321 cttttgcgat gtgaaaatct tactggtgaa aaaccatggt ccgaagcttg ggttatggga
     7381 tacggtttgg atgaggaagg gaagaagatg agcaaaagca agggcaatgc tctggatccc
     7441 ataccaatta ttgaaaagtt tggtgctgat acattcaggt tctggagtgc cagcgaggtt
     7501 aaccatggtt cagactttag atgttcggaa caaaaaatcg agtcaacgaa aaagtttctt
     7561 actaaacttt ggaatgtctc aaggtttctg tccagttttc ctgtaatcga atcagcagag
     7621 atatcagata ctgacaagtg gatcatctcc gagctcgata agcttgtaaa ggactgcaag
//
LOCUS       DASCPJ010000093         4004 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001491230, whole genome shotgun sequence.
ACCESSION   DASCPJ010000093 DASCPJ010000000
VERSION     DASCPJ010000093.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001491230"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(104..934)
                     /gene="nadC"
                     /locus_tag="QGF01_04370"
     CDS             complement(104..934)
                     /gene="nadC"
                     /locus_tag="QGF01_04370"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HJN20159.1"
                     /translation="MVIDVKKELLRFLAEDAHSYDITSALLPNQKINAQIISRDDGII
                     AGIKFAKDIFKLKGIHVKSFKPDGSKIKVNQKILQITGNSRSILSCERTVLNLLSRMS
                     GIATQTNFLVTQIKKHSKKTKLYSTRKTAPGLRYFDKVAVRIGGGYKHRLSLNDMIMI
                     KDNHLLLSNSMEEIIKKARKKRKRVEVEVENQNDSILAAKCGATVVMLDNFSPTQIKK
                     TIASLERKKLRDKVQLEASGGINAKNITAYAKTGVDMISVGSVTNSVIGLDLSLDVIK
                     "
     gene            complement(936..1124)
                     /gene="spt4"
                     /locus_tag="QGF01_04375"
     CDS             complement(936..1124)
                     /gene="spt4"
                     /locus_tag="QGF01_04375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HJN20160.1"
                     /translation="MVREMACRKCKFVTVGKVCPACKSSDLTPDWQGVVLIVNPEGSR
                     IASTLGISVKGKYALKVT"
     gene            complement(1126..1755)
                     /locus_tag="QGF01_04380"
     CDS             complement(1126..1755)
                     /locus_tag="QGF01_04380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HJN20161.1"
                     /translation="MFSISTLGDVVRIPPDLFGTSLKKAAINILKSKYESMVNSNLGY
                     IIMILDAKVDPMGKVIAGNGGTFHRVTFDALTFYPKLQEIVNGEIVDITEFGAFVRIG
                     PTDALLHLSQVMDDYLKSDVASGMILANQSGRTLKVGSTIRTRITAVSLGKAATMGKI
                     GITCRQPFLGAPDWIKEELKNSNAPEEKENVVEAKEKSKPKEKESSQEK"
     gene            1912..3003
                     /locus_tag="QGF01_04385"
     CDS             1912..3003
                     /locus_tag="QGF01_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HJN20162.1"
                     /translation="MIGIDDVLNKLTTVIDPDLKKDIVSMGMIKDLELKSGNLKFTLE
                     LTTPACPFNDEIEQDVRNAIKELDEIKNFDMNVTAKVMEGRDLAADEAMSTVKNIIAV
                     ASGKGGVGKSTVSLNLALALSKAGAKVGLLDADIYGPSIPLMLGMTKTPMQVEDNKLQ
                     PPESNGVKVVSFGFFAEQEHQAAIYRGPVISGIVKQFLIDTNWSDLDYLIVDLPPGTG
                     DIPLTLAQTIPITGILVVTTPQQVASSVAVKAIGMFDKLNVPMLGVVENMSYFECSKC
                     NEKHHIFGKGGAERISEKHNIPFLGAIPLNSGIMEGSDLGKPIMLTQPDSPSAEAFTS
                     TAKNIAAQCSIVAAKLQEEMQKEEAPTAS"
     gene            3010..3507
                     /locus_tag="QGF01_04390"
     CDS             3010..3507
                     /locus_tag="QGF01_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HJN20163.1"
                     /translation="MKLPENLREDLKIPLGDLINDIDVNNKNIQTKINPKSVLITVGD
                     RTTENMLQLGLKPQIQIIDGLEKRNQRIVSTDDAVNTNLSCRNPPGEITKESIQVIQK
                     AFSSEPPVRITVDGEEDLLVLPVCIAAPENSVVMYGQPNEGLVIVHITPEIRAKVQKI
                     LDVMN"
     gene            3640..4002
                     /locus_tag="QGF01_04395"
     CDS             3640..4002
                     /locus_tag="QGF01_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20164.1"
                     /translation="MKLDPDMRLRIYEMIGIYASRFSIPEPKILLSTREVLDMPREIT
                     EGARTSAYKYLGLSYNNQSLIFINVRKISDEKILENTIVHELIHQRFPYLSHGKRFNK
                     LVRQGLRGKRFLAYQKRK"
BASE COUNT         1299 a          703 c          710 g         1292 t
ORIGIN      
        1 tatatgatga caaatatgag gtgaaggtag gaaaaaagat agctgataaa gctcgtttgg
       61 caattgaaag gatgctttca attagctagc aagtaataca atattatttt ataacatcta
      121 ggcttaggtc taatccaatg acagaatttg taacacttcc aactgaaatc atatcaacac
      181 cagtttttgc ataggcagtt atgtttttgg catttattcc accagatgct tctaactgta
      241 ctttgtcacg cagttttttc ctttcaagtg aagctatagt ttttttaatc tgcgtaggtg
      301 aaaagttatc caacataact actgtggcac cacatttggc agcaagtata gaatcatttt
      361 gattttcaac ttcgacctcc acacgtttac gtttttttcg tgcttttttg ataatctctt
      421 ccatcgagtt agataacaaa agatgattgt ctttaatcat tatcatgtca tttagtgata
      481 gtctatgttt gtatccaccc ccaattctca ctgccacttt gtcaaaatat ctaagaccgg
      541 gtgcagtttt tctagtggag taaagcttcg tttttttact gtgttttttg atttgtgtga
      601 ccaagaaatt tgtttgtgta gcgattccac tcattcttga cagtagattt agtactgttc
      661 tttcacacga taaaatagat cttgaatttc ctgtaatttg tagaattttt tgatttactt
      721 ttattttaga accatctggc ttaaacgatt taacatgtat tccttttaat ttgaaaatat
      781 ctttggcaaa tttaatacca gcaattattc catcatctct tgaaattatt tgagcattaa
      841 ttttttgatt tggtaataag gcactagtta tatcataaga gtgtgcatct tcggccagga
      901 atctgagtaa ttcttttttt acatcaataa ccaatttagg ttaccttgag tgcatatttt
      961 ccttttacag atattcccaa agtactggcg atcctagatc cttctggatt gacaatcaga
     1021 actactccct gccagtcagg tgtgagatct gaagacttgc atgccggaca aactttgcca
     1081 acagtgacaa atttacattt tcgacatgcc atttctctaa ccatattatt tctcctgtga
     1141 actttctttt tcttttggtt tgctcttttc ttttgcttct actacgtttt ctttttcttc
     1201 tggagcatta ctatttttta attcttcttt gatccaatca ggtgcaccaa ggaatggttg
     1261 tctacatgtg attccaattt tacccatagt tgcagcctta ccaagtgaaa ctgcagttat
     1321 tctagttctt attgttgatc caacctttag agttctacca ctttgattag ctagtatcat
     1381 accggatgcc acatcacttt tgagataatc atccataact tgtgataagt gtaataatgc
     1441 atcagtagga ccaattcgaa caaaagcacc aaattcagtg atatcaacaa tttcaccatt
     1501 gacaatttct tgtaattttg gataaaatgt taaagcatca aatgtcacac gatgaaacgt
     1561 tccaccatta ccagcaatca cttttcccat tggatcaact tttgcatcta gtatcatgat
     1621 tatgtatcca aggttagaat ttaccatgct ttcatatttt gatttgagta tgttgatagc
     1681 tgcttttttc agtgacgtgc caaaaagatc aggtggaatt ctaacaacat cacctagagt
     1741 agaaatagaa aacacttcga aatcattcac gattttcaat ttatgaacct ttgggtaaat
     1801 tttcacgtat ttctatacaa aatagcaatt tgagaagacg agagtagaat tatgaaatta
     1861 agccaagcta aatcagccta tctttaaata aagttcgagc caatttcgca tatgattggc
     1921 attgatgatg ttttaaacaa attaacaact gttattgatc ctgatctcaa aaaagatata
     1981 gtttcaatgg gaatgatcaa agacctagag ctaaagtctg ggaatctaaa gttcacactt
     2041 gagcttacca cgccagcatg cccatttaat gatgaaatag agcaagatgt acgtaatgca
     2101 ataaaagaac ttgatgaaat taagaacttt gacatgaatg taactgcaaa agttatggaa
     2161 ggtcgtgatt tagctgctga tgaagcaatg tctactgtga aaaatattat tgcagtggca
     2221 agtggtaaag gtggagttgg taaatccact gtatcattga atcttgcatt ggcactttca
     2281 aaggcaggtg ctaaagttgg attgttagat gctgatatct atggtccaag cattccatta
     2341 atgcttggaa tgactaagac accaatgcag gttgaagaca ataaattaca accacctgaa
     2401 tcaaatggag ttaaggttgt ttctttcggt tttttcgctg aacaggaaca tcaggcagca
     2461 atttacaggg gaccagtcat ctctggaatt gtaaaacagt ttttaattga taccaattgg
     2521 tctgacttgg attatctaat agttgattta ccaccaggta ctggtgatat accattaact
     2581 ctagcacaaa caattccaat tacaggaatt cttgtagtta caactccaca gcaagttgca
     2641 agtagtgtag cagttaaggc aattggaatg tttgataagc ttaatgttcc gatgttagga
     2701 gttgttgaaa atatgagtta ctttgaatgt tcaaaatgta atgaaaaaca tcacatattt
     2761 ggaaaaggtg gtgcagaaag aattagcgaa aaacataaca ttcctttcct tggtgcgata
     2821 cctctgaact ctggaattat ggaaggttct gatttaggta aaccaattat gcttacacaa
     2881 ccagattcac caagcgcaga agcatttact tcgactgcca aaaatattgc agcgcagtgt
     2941 agtattgttg ctgcaaagct acaagaggaa atgcagaaag aagaagcacc cacagctagt
     3001 taggatttct tgaaacttcc tgaaaacctt cgtgaagatc ttaagatacc attaggggat
     3061 ttaatcaacg acatagatgt aaataataaa aatattcaaa ccaaaattaa tccaaaatca
     3121 gttctaatta cagtaggaga tagaacaact gaaaatatgt tacaattggg tctcaaaccg
     3181 caaattcaaa tcatagatgg tctcgaaaaa cgtaatcagc gtatcgtatc tactgatgat
     3241 gcagtaaaca cgaatctttc atgcagaaat ccacctggtg aaataaccaa ggaaagcata
     3301 caagttatcc aaaaagcatt ttctagtgaa ccaccggtta gaattacagt agatggagag
     3361 gaagatcttc ttgttttacc tgtatgcatc gcagcacctg aaaattctgt agtcatgtat
     3421 ggtcaaccta atgaaggctt ggtaattgtt cacatcacac ctgaaatacg ggcgaaggtt
     3481 caaaaaatac ttgatgtaat gaattagggg tatgatgaga cggtggctgt atgaattatg
     3541 attacactac taaactctga ccctactctg taaacctaaa ctcagatcga aataacaatt
     3601 gacatcgtat gataaaccat aatccaaaga tatgttctca tgaaattaga tcctgacatg
     3661 agactaagaa tttatgaaat gattggaatt tacgctagcc gtttttccat ccctgaacca
     3721 aaaattcttc tatccacacg tgaagttctg gatatgccaa gagagataac tgaaggtgct
     3781 aggacttcag catacaaata tctgggtctt tcatataaca atcagagcct aatattcatc
     3841 aatgtcagga aaatttctga tgagaagatt ctggaaaata cgatcgttca tgagctgatc
     3901 catcaaagat ttccatatct tagccatgga aagaggttta acaaattggt ccggcaaggt
     3961 ttacgcggaa aaagattctt ggcatatcaa aaaagaaaat aagt
//
LOCUS       DASCPJ010000094         6137 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001494680, whole genome shotgun sequence.
ACCESSION   DASCPJ010000094 DASCPJ010000000
VERSION     DASCPJ010000094.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001494680"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="QGF01_04400"
     CDS             <1..620
                     /locus_tag="QGF01_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HJN20165.1"
                     /translation="TKFAKDNGPFTWLAQGTLYPDVIESGVSKGPASVIKTHHNVGGL
                     PDWLELKVLEPLRDLYKDEVRKVAKILDVPENLIKRHPFPGPGLAVRIIGEVNQHKLE
                     ITRMASKIVEEELRNAELYDNIWQAFAIVGDDRAVGVVGDERKYGHVVMIRIVNSVDA
                     MTADWVRLPNELLEKISNRITNEIDEVTWVSYVISSKPPATIEPQ"
     gene            complement(621..1499)
                     /locus_tag="QGF01_04405"
     CDS             complement(621..1499)
                     /locus_tag="QGF01_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HJN20166.1"
                     /translation="MHKIGIDLGGTKIEGILVDEKFNLIERKRIETHQESGYDSIVKS
                     ITSLVSELKSKTNVDTSVGVCTPGISDTNSGLIKNSNTQCLIGMPLKTDIEKRLGHKI
                     FMENDANCFALAESSLGAGKGYNIVFGVILGTGVGGGIVINGSLHKGRTNIAGEWGHH
                     TLHPNGRQCYCGKQGCVETYISGPALENNWSVLTGKKESLQIITENHSDEKKEEWKRN
                     FLENFGIGIANVIDILDPDAIILGGGVSNIPFLYDEGKKMIFEKVFSDSVDTPILKNK
                     LGDSAGVFGACLLDDL"
     gene            complement(1549..3168)
                     /gene="thsB"
                     /locus_tag="QGF01_04410"
     CDS             complement(1549..3168)
                     /gene="thsB"
                     /locus_tag="QGF01_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HJN20167.1"
                     /translation="MASIQQTPNGPVLVLKESALQQKGKDAQKNNITAAKLVAQLVKT
                     SLGPRGLDKMLVDSLGDVTITNDGATILKEIDAQHPAAKMMVEVSKTIDTEVGDGTTS
                     SVVIAGSMLEKAEKLLEKNIHSTVIIDGYQNAAEKALELLKGLAKKIQADDHESLTKI
                     AKTSMQSKLISEDSVGLSKLVVDSILKIARKDGENYSVDLDNLKVEKKSGGSIDDTTL
                     IKGIVLDKEIVHSGMPTKIEKAKIALVNAALEVEKTEMSAEIRISDPTQMQMFLEEEN
                     RMLKDMVEKLHQVGANVLICQKGIDDIAQHFLSKHGILAVRRVKESDMTKLTKATGGR
                     ITTNLDDITTNDLGSADIVKQKKVESDKWVFIEGCRNPHSVTVLIRGGSQRVVDEVDR
                     SIHDALMVVKDVIETPSIVAGGGSPEAYLSSQLKEWADTFDGREQLAIKQYAESLEAI
                     PLTIAENAGMDPIDTIISLRARQASGKKSIGINAKEGKIGDMYSLDIVEPLVVKEQII
                     KSATETACMILRIDDVISISGGSAGPGGPMG"
     gene            complement(3207..4973)
                     /locus_tag="QGF01_04415"
     CDS             complement(3207..4973)
                     /locus_tag="QGF01_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HJN20168.1"
                     /translation="MHSGLSRSISALNSVATGSKPADLVLHNCSLVNVYTREIVPDTE
                     IAISQGRIAYVGKNASHTKGKQTVIIDLEKKFVTPGFADPHIHLDQFVTPLEFVKKSI
                     LCGVTSLFADSIDIASTCGYRGFSEFVKMTSELPVRIFHVIPGGMPVDRKFSHGKTFS
                     ISEEKSAMKIPDVIGLGEVFSWTKVTQRDPKTMKTLSAMLERNLVINGHTAGLADKKL
                     NAYVSSGILSCHEPINFDQVLERLRLGMWIMIREGSIRRDLTEIIKFVHSNGVYTDRL
                     MFCSDGLDPIDLTKFGHIDHCVRESVKAGLNQVDAISMASKNCFDYYNMGKDLGGIAP
                     GKLADILVFDDLNKLKPNKVFIGGRLVVSNGSIVSKIKKYTIPKWMTKTIKLKKITEN
                     DFLVKSKSDLVNVNVIDMKTEIVTEKSSESLSVYNGNVLASHEKDVWKVAAFDRISGS
                     AKHTVAFLRNFGAEIGAFASTWSFHENDMIVIGSNEKDMAIAANHLAKTQGGMIVVNN
                     GKILSFMPLQMAGIVSTDNFEKVTENFENISQILTDRGCKFKKPHLIPLFLPFLALPS
                     IRILFSGLIDVKTRSFLKVVTG"
     gene            5048..5329
                     /locus_tag="QGF01_04420"
     CDS             5048..5329
                     /locus_tag="QGF01_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HJN20169.1"
                     /translation="MNLPKAKNTFCPKCNKHTIQKISIYKAGKRRGMAAGERRHAQRK
                     KGYGGQKFPKLAKPAKTTKKVTQVLTCPDCNKKMMKKGIRIRKFELVAK"
     gene            5326..5523
                     /locus_tag="QGF01_04425"
     CDS             5326..5523
                     /locus_tag="QGF01_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HJN20170.1"
                     /translation="MKREHIIIPEPNSKFLKVNCKECDTENVVFSHASTPITCKSCGN
                     VIVKPTGSKAKIIGKVSGTAE"
     gene            complement(5506..6090)
                     /locus_tag="QGF01_04430"
     CDS             complement(5506..6090)
                     /locus_tag="QGF01_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HJN20171.1"
                     /translation="MIGLVMAGGKGTRMDHSGEKLLLKYKKPVVINVIDSLQNSNCFS
                     KVIVSTSPNSPNTKSVLEQNGIETLSTPGNGYTNDLNSLLQKMEGFLFVISGDLPLLD
                     EKIIQIMTEKFNPENVWTSFLVSKNFLNSLNLESDLLINYDNIECVYTGISIINADKI
                     KNLNLVKETYIILDDKRIAFNLNTKKDYELLSSS"
BASE COUNT         1929 a         1135 c          966 g         2107 t
ORIGIN      
        1 ttaccaagtt tgcaaaagat aatggtccgt ttacatggct tgcacaagga acactttatc
       61 ctgatgtaat tgaaagtgga gtgtcaaaag gacctgcatc tgtgatcaaa acacatcata
      121 acgtaggagg attgccagat tggttagaac ttaaggtttt agaaccatta cgtgatttgt
      181 ataaagacga agtaagaaag gtagcaaaga ttcttgatgt accagaaaat ttgatcaagc
      241 gccatccatt tccaggtcct ggtcttgctg taagaataat tggtgaagtt aaccaacata
      301 aattagagat tactagaatg gcaagtaaaa tagtagaaga agagttacgg aatgcagaat
      361 tatatgataa tatttggcaa gcgtttgcta tagtaggtga tgatagggct gtcggtgttg
      421 ttggagatga aagaaaatac ggtcacgttg tgatgattag aattgtaaat tcagttgatg
      481 ccatgactgc tgactgggtc agactaccaa atgaattatt agagaaaatt agtaatcgta
      541 taacaaatga gatagatgaa gtgacatggg tttcctatgt gatctcaagt aaacctccag
      601 ccacaataga acctcaataa tcacaaatca tctagtagac aagcaccaaa tactccagca
      661 gaatcaccaa gtttattttt tagaattggt gtatcaactg aatcactaaa caccttctca
      721 aatatcatct ttttaccctc atcatatagg aatggaatgt tagaaactcc tccacctaaa
      781 ataatggcat caggatctaa gatgtcaatt acgtttgcaa taccaattcc aaaattttct
      841 aaaaaatttc ttttccactc ttccttcttt tcatcagaat gattttcagt tatgatttgt
      901 aatgattctt tttttccagt aagtactgac caattattct ctagtgctgg accgctaata
      961 tacgtctcaa cacatccttg ttttccacaa taacactgtc ttccattagg atgtaatgtg
     1021 tgatgtcccc attctccagc aatatttgtt ctacccttat gtaacgatcc gttgattact
     1081 attcctccac caacacctgt tcctaatatt actccaaaaa ctatattgta tccttttcca
     1141 gcacccaagg acgattcggc aagggcaaaa caatttgcat cattttccat aaaaattttg
     1201 tggcctaacc ttttttcaat atctgttttt aacggcattc caataagaca ttgtgtatta
     1261 ctgttcttaa ttagcccaga attggtatca ctgatacctg gcgtacatac accaactgat
     1321 gtatctacat ttgttttaga ttttaattca cttaccaaag atgtaataga ttttacaata
     1381 gaatcataac cactctcttg atgggtttca atgcgtttac gttcaattaa attaaatttt
     1441 tcatccacta aaattccttc aatcttagtt ccacctagat caattcctat tttatgcaca
     1501 agtacagttt attgcgtaag ttttgaagtt ttggtaaaaa atctataatt atcccattgg
     1561 accgcctggt ccagcactac caccagaaat agagattaca tcatctattc tgagaatcat
     1621 gcatgcagtt tccgtggctg atttgataat ctgttctttc actactagtg gttctacaat
     1681 atctaatgaa tacatatcac caatcttacc ctcctttgca ttaattccaa tacttttttt
     1741 gccactcgct tgccttgcgc ggagagagat aatagtatca attggatcca ttcctgcatt
     1801 ttcagctatt gttaatggaa tagcttctaa ggattcagca tattgcttaa tcgctaattg
     1861 ttctctacca tcaaatgtat cagcccattc ttttaattgt gaagatagat atgcttcagg
     1921 tgaaccacct ccagctacta tagatggagt ttcaattaca tcttttacaa ccattaaggc
     1981 atcatgaatt gagcggtcaa cttcatcaac aactctttgt gaacctcctc tgattagaac
     2041 tgttactgaa tgtgggtttc tacagccttc gatgaatacc catttatcag attcaacctt
     2101 tttttgtttt acaatatcag cgcttccaag atcattagta gttatatcat caagattagt
     2161 agtgattctt ccacctgtag cttttgttaa tttagtcata tcactttctt ttactcttcg
     2221 tacagctaaa attccatgtt tagatagaaa atgttgtgct atgtcatcaa tgcccttttg
     2281 acagattaga acattggcac ctacttgatg taatttttca accatgtctt tgagcattct
     2341 attttcttct tccaagaaca tttgcatttg agttggatca gaaatcctaa tttcagcact
     2401 catttcagtt ttttctactt caagagcagc attaacaagt gcaatttttg ccttttctat
     2461 ctttgttggc atgccactat gaacaatttc cttatcaaga actatacctt tgattaaagt
     2521 tgtatcatcg atagaaccac ctgatttttt ttctactttt agattatcaa gatcaactga
     2581 atagttttca ccatctttac gtgcaatttt tagaatagaa tcaacaacta gtttagacaa
     2641 accaacacta tcttcagaaa ttaatttgga ctgcatactg gtttttgcaa ttttggttaa
     2701 tgattcatgg tcatcagcct gtattttttt tgccaatcct tttaacaatt caagagcttt
     2761 ttcagcagca ttttgatatc catcaataat tactgtggaa tgaatatttt tttcaagtag
     2821 tttttctgcc ttttctaaca tagaacctgc aataactaca gaagatgttg ttccatcacc
     2881 tacctcagta tcaattgtct tagaaacttc gaccatcatt tttgcagctg gatgctgagc
     2941 gtcaatttct tttagaatag tggctccgtc gtttgtaatt gtcacatcac ctaacgaatc
     3001 caccaacatt ttgtctaaac cgcgtggtcc tagactagtt ttaactaatt gagcaactaa
     3061 tttggcagca gtaatattgt ttttttgtgc atctttgcct ttttgctgta atgcactttc
     3121 ctttaatact aaaactggac cgttaggagt ttgttgaata gatgccatta atttacagat
     3181 tttttttata tgttcccttt ataattttat ccagtaacaa ctttgagaaa tgaacgcgtt
     3241 tttacatcaa ttaaaccact aaacagtatt cggatagatg gaagtgctaa aaatggtaag
     3301 aaaagaggta ttaggtgagg ttttttaaat ttacagcctc tatcggttaa tatttgtgat
     3361 atgttttcaa agttttctgt aactttttca aaattatctg tagaaacaat acctgccatt
     3421 tgtaaaggca tgaaggaaag gattttacca ttatttacta ctatcatacc tccttgtgtt
     3481 ttggcaagat gattagcggc tattgccatg tctttctcat ttgaacctat tacaatcata
     3541 tcattctcgt gaaaactcca agttgacgcg aatgcaccta tttcagcacc aaaattcctt
     3601 aggaatgcca cagtatgctt tgctgagcct gatatcctat caaaagcagc aactttccat
     3661 acatctttct catgggatgc caaaacattt ccattataaa cagataaaga ttcactactc
     3721 ttctctgtga ctatctctgt tttcatatca atcacattaa cattaaccaa atcggatttg
     3781 ctcttgacta ggaaatcatt ctctgtaatt ttttttaatt ttatagtttt tgtcatccat
     3841 tttggtatgg tgtatttttt tatttttgaa acaatactac cattagatac tactaatcgg
     3901 cctccgatga aaactttgtt gggtttcagc ttattcagat catcaaaaac taaaatatct
     3961 gctagcttac ccggtgctat tccccctaaa tctttgccca tgttgtaata atcaaaacag
     4021 ttttttgatg ccatagatat tgcatcgacc tgattaagac ctgcctttac tgattctcta
     4081 acacagtgat caatatgacc aaattttgtt agatcaatag gatctaagcc gtctgaacaa
     4141 aacatcaatc tgtccgtata aactccattt gaatgaacaa attttatgat ctcggtaaga
     4201 tctctgcgaa ttgatccttc cctgatcatt atccacattc ctaatcgcaa tcgttctaaa
     4261 acttgatcaa agttaatcgg ttcatgacat gataaaattc cagatgagac gtaagcattt
     4321 agtttcttat cagccaagcc cgcagtatgt ccatttatca ccaaattcct ttcaagcatt
     4381 gctgataatg ttttcatggt tttaggatca cgttgtgtta ccttagtcca tgaaaatact
     4441 tcgcccaagc caataacatc aggtatcttc attgcagatt tttcttcaga tatactgaat
     4501 gtttttccgt ggctgaactt tctatcaaca ggcatacctc caggtattac atgaaaaatg
     4561 cgtactggaa gctcagatgt cattttgaca aattcactaa aacccctata cccacatgtg
     4621 cttgctatat caattgaatc agcaaaaagt gaagtaaccc cgcataggat agattttttt
     4681 acaaattcta gtggtgtgac gaactgatcc agatggatgt gaggatcagc aaatccagga
     4741 gtaacaaatt ttttttctaa atctatgatt acagtctgtt ttccttttgt atgagatgca
     4801 tttttcccaa cataagctat tctgccttgt gatatagcta tttcagtatc tggtacaatc
     4861 tcacgagtat acacattgac tagtgagcaa ttatgcaata caagatcagc cggttttgag
     4921 ccggtagcaa ctgagtttag agctgaaata gatctagata accctgaatg cattattgat
     4981 cttaacccgt tgaacttatt atagattaga agcgatgttt aaattacagt cagattcgga
     5041 ataaattgtg aatctaccaa aagcaaaaaa tactttttgt ccaaaatgta ataagcatac
     5101 tattcaaaaa atctctattt acaaagcagg taaaagacgt ggaatggctg caggcgaaag
     5161 aagacatgcg caaagaaaaa agggatatgg tggtcagaag ttcccaaagc ttgcaaagcc
     5221 tgcaaaaaca acaaagaaag ttacacaggt tttgacgtgc ccagattgta ataagaagat
     5281 gatgaaaaaa ggtatcagaa taagaaaatt tgagttggtt gcaaaatgaa gagagagcat
     5341 ataattatac cagaaccaaa tagtaaattc ttaaaagtta attgtaaaga atgtgacact
     5401 gaaaatgtag ttttttcaca tgcttctaca cctataactt gcaaatcctg tggaaatgtt
     5461 atagtaaaac caactggctc aaaagcaaaa ataattggta aagtttcagg aactgctgag
     5521 taactcgtaa tctttctttg tgttcaaatt aaaagctatt ctcttatcat ctaaaattat
     5581 gtaagtttct ttaactagat tcaaattttt aattttgtca gcatttatga tagaaattcc
     5641 agtatacaca cattcaatat tatcataatt aatcaataga tcagattcta gattcaagga
     5701 gtttaaaaaa tttttagata caagaaaact ggtccaaaca ttttctggat taaatttttc
     5761 agtcataatc tgaatgattt tttcatctag tagtggtaag tccccagaaa taacaaacaa
     5821 aaatccttcc attttttgta gtagagaatt tagatcattg gtatatccat ttcctggtgt
     5881 ggataaagtt tctattccat tttgttctaa tacagatttt gtattgggtg agtttgggct
     5941 tgtagataca ataactttag aaaagcaatt agaattttgt agtgaatcaa ttacattgat
     6001 gactactggt tttttatatt tgagtaataa tttttcacca gaatgatcca tcctggttcc
     6061 tttgcctcca gccatgacta atccaatcat aatgatacaa agattagcag agatgatagc
     6121 cttgtaagtt cattagc
//
LOCUS       DASCPJ010000095         3518 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001528031, whole genome shotgun sequence.
ACCESSION   DASCPJ010000095 DASCPJ010000000
VERSION     DASCPJ010000095.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001528031"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..72)
                     /locus_tag="QGF01_04435"
     tRNA            complement(1..72)
                     /locus_tag="QGF01_04435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Gln,seq:ctg)
     gene            complement(103..423)
                     /locus_tag="QGF01_04440"
     CDS             complement(103..423)
                     /locus_tag="QGF01_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20172.1"
                     /translation="MLREKGKYATSTQNRRIVWHKIIWPLILELDDVVFSLKQYQKKR
                     DEICNKDTLKISEMSRGLVSLLQKGIILKESDMYSIHYRLIPYMRVKAECDYSTAMNE
                     VRMK"
     gene            454..1158
                     /locus_tag="QGF01_04445"
     CDS             454..1158
                     /locus_tag="QGF01_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HJN20173.1"
                     /translation="MSQSEIAKSLELLQKDWDIDPIIKDFHLGKRDDISENSIKVKDV
                     IFHIPFLNKIKKFILWKCYWPDCSNCCSRQGRLPLTSDDLITIGTGMKYQKTSDFIKN
                     ETVIATWQEPSPGGGFTTLTGINLKRKEDETEADDGTHIKCRFLDEQGACNIHPTRPG
                     VCYLYPFSTWLQNEKGKPRVHATFQFTGDCPGFYLDDSINPMKEILQEYSEIIYNYNI
                     KSNQTTREGLGSISLG"
     gene            complement(1155..2165)
                     /locus_tag="QGF01_04450"
     CDS             complement(1155..2165)
                     /locus_tag="QGF01_04450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJN20174.1"
                     /translation="MKIAVVGIGVAGSYLLSRLKKDHEVIGYERMTQENHDSICAWGT
                     IKSSMSELCSKSDIDFEKHIIHDGKNLYIDMNNQQRFDIKLKGLCTYDKIGLINDMAK
                     GCKIHYDVEPKLEDMEKEFDLIIDATGFYRPYLPKIKKDFFLPTYQYKILFENEVPLD
                     DFYVKPFPGMTGYFWYFPLGKNMAHIGAGDYKKQHVQQTNEFLKKYGGKILKTVGRPI
                     RLATPDLCEPFYHGKVVGVGESIGTVYPLLGEGIIPSMVCADLFVKNIGNNEQYRKDV
                     LKYFSVYAKVLHFIKTKMKGEFSAMKQLGELFKIYRYMKKNEERFGMEIHMRDLMKVA
                     KA"
     gene            complement(2209..>3518)
                     /locus_tag="QGF01_04455"
     CDS             complement(2209..>3518)
                     /locus_tag="QGF01_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HJN20175.1"
                     /translation="ISKFVNAKEILAENMSDEFFDYVFYEKGNSDAVAQKMQITVKKL
                     DEIRKEFLYFYPVDSRHSGRDLVPNHLTFFVLNHVVLFPKENWPKEIVVNGSVLMDGK
                     KMSKSMGNIIPLRDAIKKYGADPIRLSILISAELLQDADFNIESVSGIKNKLEVMLEE
                     CKKSKKSECADLEPEDVWIQSRLSHLISEVTESMDKMRFREALHHIVYEFDSELQWFL
                     KRIKAKKRENVTGIMYKILSVRVAMLSPFAPHIAEEMWEQLGNTKMVSKSTWPIQEIQ
                     DNNTPVLTESLLSSTMNDIANIIKVTKISPKRIVIYTADSWKSKAYHQILKNVHSGQT
                     DIGTLIKELIANKDTEQIKKDPDFVKKTLKDILSEPTELRDAKMTAGEIDENASISSE
                     LTSLIENDYGIKLQVFSEDDQDKYDPKNKARIARPYKPAILIE"
BASE COUNT         1079 a          634 c          513 g         1292 t
ORIGIN      
        1 tagcccggcc cggactcgaa ccgaggtcaa aggctccagg ggcccctatg cttgccacta
       61 cactaccggg ctacaagaac atttgtaaaa tatagaatat aattatttca ttctaacttc
      121 attcattgca gttgaataat cgcattctgc tttgactctc atataaggaa ttaatctgta
      181 atgaatcgaa tacatatcac tttctttgag gattattcct ttttgtaata atgatactaa
      241 tccacgcgac atctctgaga tttttaatgt atctttattg catatttcat cacgtttttt
      301 ctgatattgt ttaaggctga atactacatc atcaagttct aagattaatg gccatatgat
      361 tttgtgccag acaatccttc tattttgggt agatgttgca tacttgcctt tttctctcaa
      421 cattaataat atgattttgc actattataa ttcgtgtccc agagcgagat tgcaaaatca
      481 ttagaacttt tacaaaaaga ttgggacata gacccaatca taaaagattt tcatttggga
      541 aagcgtgatg atatatctga aaattccata aaagtgaaag atgttatatt tcatattcca
      601 tttctaaaca aaattaaaaa attcatactt tggaaatgtt attggcctga ttgttcaaat
      661 tgttgtagta gacaaggaag acttcctttg acttctgacg atttgataac aattggaact
      721 ggaatgaaat atcaaaaaac atctgatttc ataaaaaatg aaacagtgat agctacttgg
      781 caggaaccaa gtcctggtgg tggctttacc acattaactg gtattaattt gaagcgaaag
      841 gaagatgaaa cagaagcgga tgatggtact catatcaaat gtagatttct agatgaacaa
      901 ggtgcatgta atattcatcc aacacgacct ggtgtatgtt atctttatcc attttcaaca
      961 tggttacaaa atgaaaaagg caaaccaagg gttcacgcta cttttcaatt tactggtgat
     1021 tgtccaggtt tttatctaga tgattcaatt aatccaatga aagaaatctt acaagaatat
     1081 tctgaaataa tatataatta taatataaaa tctaaccaaa ctacgcgtga agggttaggc
     1141 tcaatcagtt taggttaggc ttttgcaact ttcatcaaat ctcgcatatg aatttccatc
     1201 ccaaatcttt cttcattctt cttcatatac ctgtatattt tgaataactc accaagttgt
     1261 ttcattgcac tgaattctcc cttcattttt gtcttaataa aatgaagtac tttagcataa
     1321 actgaaaaat actttagaac atcttttcta tactgttcat tatttcctat atttttgaca
     1381 aacaaatctg cacaaaccat actaggtata ataccctcac ccaataatgg atatacggtc
     1441 ccaattgatt caccgactcc tactacttta ccatggtaga atggttcaca taaatcagga
     1501 gttgctagac gtataggccg ccccaccgtc ttgagaatct ttccaccata tttttttaaa
     1561 aattcattgg tttgttgaac atgttgtttt ttataatcac cagctcctat atgggccata
     1621 ttttttccta atgggaaata ccaaaagtaa ccagtcatac ctggaaatgg tttcacataa
     1681 aaatcatcaa gaggaacttc attttcaaat aatattttat attgatatgt aggcaggaag
     1741 aagtcttttt tgatttttgg aagatatggt cgataaaatc cagtagcatc aattatcaga
     1801 tcaaattctt tttccatgtc ttccagtttt ggctctacgt catagtgaat cttacaacct
     1861 tttgccatat cattaatcaa cccaatttta tcatacgtac ataatccctt caattttatg
     1921 tcaaatcttt gttggttatt catgtctatg tatagatttt ttccatcatg aatgatatgt
     1981 ttctcaaaat cgatatcaga ttttgagcag agttcactca tactactttt tattgttccc
     2041 catgcacaaa ttgaatcatg attttcttga gtcattcttt catatcctat aacctcatga
     2101 tcttttttta gtctagaaag aagataacta ccagccacac ctattccaac aactgcaatc
     2161 ttcatttgat ttttaggtaa atattaccca ataaatacaa ttctagtttt attctattaa
     2221 aattgctggt ttgtaaggtc tagctatacg tgctttgttt tttggatcat atttgtcttg
     2281 gtcatcttca gaaaatacct gtaatttaat tccataatca ttttcaatta aagatgtaag
     2341 ctctgatgaa attgatgcat tttcatcaat ttctccagca gtcattttag catctctaag
     2401 ttccgttggc tctgatagaa tatccttaag agtttttttt acaaaatctg ggtctttttt
     2461 tatctgctct gtatccttgt tcgcaatcaa ttctttgatt aacgtcccaa tgtctgtttg
     2521 cccagaatga acattcttta gaatttgatg gtatgccttt gatttccaag agtcggctgt
     2581 atagattaca attctttttg gagagatttt tgtcactttg attatatttg caatatcatt
     2641 cattgtagaa cttagtagac tctcagttag tactggtgta ttgttatctt gtatttcttg
     2701 aattggccat gtagattttg ataccatttt tgtatttcca agttgttccc acatttcttc
     2761 tgcaatatgt ggtgcaaacg gagaaagcat ggcaacacgt actgacaata ttttatacat
     2821 gattccagta acattttctc ttttttttgc cttgattctt ttcaagaacc attgaagttc
     2881 agagtcgaat tcgtaaacta tatgatgtaa cgcttcacga aagcgcattt tatccattga
     2941 ttcagttacc tctgaaatca aatgtgatag ccgactttga atccagacat cttctggttc
     3001 taaatcagca cactcagatt tctttgattt tttacattct tcaagcatta cctctaactt
     3061 atttttgatt ccagagactg attcaatatt aaagtcagca tcttgtaata attcagctga
     3121 aattagaatt gaaagcctaa ttgggtctgc accatatttt ttaattgcat cacgtaatgg
     3181 tatgatgttt cccatagatt ttgacatttt ttttccatcc atcagaactg atccgtttac
     3241 aacaatctct ttcggccaat tttcttttgg gaataacact acatggttta atacaaaaaa
     3301 tgttagatga tttggtacca aatctcttcc agaatgtctt gaatcaacag ggtaaaaata
     3361 caaaaattct ttccttatct catcaagttt ttttactgta atctgcattt tttgcgcaac
     3421 agcatctgaa tttccttttt cataaaatac atagtcaaag aattcgtctg acatattttc
     3481 agccaaaatt tcttttgcat taacaaattt tgaaatta
//
LOCUS       DASCPJ010000096         1787 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001535524, whole genome shotgun sequence.
ACCESSION   DASCPJ010000096 DASCPJ010000000
VERSION     DASCPJ010000096.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001535524"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /gene="mtnA"
                     /locus_tag="QGF01_04460"
     CDS             complement(<1..760)
                     /gene="mtnA"
                     /locus_tag="QGF01_04460"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HJN20176.1"
                     /translation="MTLRTIEWKNNEVIMIDQTKLPNELVYVKFTDYNDVAEAIRTLV
                     VRGAPAIGVSGAFGMALAGLQSKAETKEELLSDLEKAQKILFMTRPTAVNLVWGLEQI
                     MKVAKQNDSVDEIKESIIDKAKQMADDDIKINREMGKHGSQLFENNDSVMTHCNAGAL
                     ATVGYGTALGVIRATKESGKNIKVIATETRPVQQGSRLTAFELKHDGIDVAIIPDTAV
                     GYTMANNLVNRVVVGADRILRTGHVYNKIGTYQVA"
     gene            859..>1787
                     /locus_tag="QGF01_04465"
     CDS             859..>1787
                     /locus_tag="QGF01_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HJN20177.1"
                     /translation="MIDRSRLDKIDSLGMCKVYDKWPEIAESNFNMDSEPIECDKIDH
                     VVFAGMGGSGSLSDIFSAILSKSNIHVSVVKGYHLPKTVNSDTLVVTTTVSGNTTETL
                     SVLNSATKQNCKVIGFTSGNKMEEYCIKNKIECRKIEQIHSPRSSFPAFLYSMLNTLK
                     PILPIEKNDVLESISHLKNQHAKISSENLSDTNPAISLAEWIVGIPVIYYPWGLEAAA
                     IRFKNSFQENAKSHAMAEDVIEACHNGIVAWEKKTNVQPVLIRGQDDFVKTVERWKIL
                     KSFFEKNGIEYKEIVSVQGNIVSKIINLIYLLD"
BASE COUNT          563 a          321 c          288 g          615 t
ORIGIN      
        1 tagctacctg atatgttcca attttgttat acacatgacc agttcgcaat attcgatctg
       61 ccccaactac aactctgttt acaagattat ttgccatagt ataacctact gctgtatctg
      121 gaataatggc aacatcaatt ccatcatgtt ttaactcgaa agcggttagt cttgatcctt
      181 gttgtacagg tctagtttct gttgcaatta ctttgatatt ttttccactt tcttttgtgg
      241 ctctaataac acctaaagct gtgccatatc ccacagtagc taacgcacca gcattacaat
      301 gcgtcattac cgaatcatta ttttcgaata attgggaacc atgttttccc atctctcggt
      361 taattttaat atcatcatca gccatttgtt ttgccttatc aattatggat tctttgattt
      421 catctacaga atcattttgt tttgcaactt tcattatttg ctccaagccc caaactaaat
      481 tgacagcagt aggtcttgtc ataaagagaa ttttttgtgc tttttctaaa tcagataaca
      541 actcttcttt agtttccgcc ttactttgta atcccgccaa tgccattcca aatgcccccg
      601 acacaccaat agcaggagca ccacggacaa ctagtgttct aattgcttca gctacatcat
      661 tataatcagt aaattttaca taaactaatt catttggcag tttagtttga tctatcatta
      721 ttacttcatt atttttccat tctattgttc ttagtgtcat ataccttcag aaaatcctaa
      781 aagaggttat aatacttgcc cagcctagta tgaataatta gttaagcatt ttattgtcat
      841 aagaaatcgt aaagtagatt gattgatcgt tcaagattgg ataagataga ttctttgggg
      901 atgtgtaaag tttatgataa atggccggag atagcagaat caaacttcaa tatggattct
      961 gagccaattg aatgtgataa gatagatcat gttgtttttg ctggtatggg aggatctgga
     1021 tcattgagcg atatattttc tgcaatatta tcaaaatcca atattcatgt atctgtagta
     1081 aaaggatatc atttgccaaa aactgtaaat tcagatacac ttgttgtgac aactactgtg
     1141 tctgggaata ccaccgaaac tctttctgta ttaaattctg cgactaaaca aaattgtaaa
     1201 gtgattggat tcacttctgg taacaaaatg gaagaatatt gtataaaaaa taaaattgag
     1261 tgtcggaaga ttgaacaaat acattcacca cgttcttcat ttcctgcttt cttgtattcg
     1321 atgcttaaca ctctaaagcc cattttaccg attgaaaaaa atgatgtatt ggaatcaatc
     1381 tcacatctaa aaaatcaaca tgccaaaatt tcatcagaaa atctttcaga tacaaacccc
     1441 gctatttctc ttgctgaatg gattgttggt ataccagtca tatactatcc atggggcctt
     1501 gaggctgctg ccattcgttt taaaaactca tttcaggaaa atgccaaatc acatgcaatg
     1561 gctgaggatg tgattgaagc ttgtcataat ggtatagttg cttgggaaaa aaagacaaat
     1621 gttcagcctg ttttgattag aggacaagat gattttgtca aaactgtaga acggtggaaa
     1681 attctaaaat cattttttga aaaaaatggg attgagtata aagaaatagt ttcagttcaa
     1741 ggaaatatcg tttccaaaat aatcaacctc atttatttat tagattt
//
LOCUS       DASCPJ010000097         4774 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001543459, whole genome shotgun sequence.
ACCESSION   DASCPJ010000097 DASCPJ010000000
VERSION     DASCPJ010000097.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001543459"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="QGF01_04470"
     CDS             <1..398
                     /locus_tag="QGF01_04470"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965801.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HJN20178.1"
                     /translation="LIHNIMIPALGGPKGKMSASNENETIYTTDSPEVVRKKINKYAF
                     SGGQPDIEEHRKKGGNPDIDVSYQYLRIFFEPDDKKLEQIHDDYKSGKMLTGELKEIL
                     VEKINQFLASHQQKREKAKNELDKFLLKD"
     gene            395..679
                     /locus_tag="QGF01_04475"
     CDS             395..679
                     /locus_tag="QGF01_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20179.1"
                     /translation="MNEILILCEDKYEAQKLSGFIFSKDEKESFISEILNVVGNEIVI
                     SLKDKSAHSVLLKDDHQVEAFVDFIQSVIEKEHKVLKLDILENSVKLTKG"
     gene            complement(682..1011)
                     /locus_tag="QGF01_04480"
     CDS             complement(682..1011)
                     /locus_tag="QGF01_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20180.1"
                     /translation="MSVTSKILNQINNDVVNLKDGLNPENISYWYEKIINETREMAPP
                     WLTDKINVKQDPILPLKFDIDISKRAVRYFMQIVDANLDSMPYSTKLYFLKVEEILSS
                     EMDKSLV"
     gene            complement(1072..1536)
                     /locus_tag="QGF01_04485"
     CDS             complement(1072..1536)
                     /locus_tag="QGF01_04485"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HJN20181.1"
                     /translation="MKLFDLVAMGGTFDIIHSGHMALLNKAFSISTKVIIGVTSDQLA
                     VKNGKKPGNDYSKRISLLKSTIEENFPDSAYEIARLENEFGPAVLEEYVKALIVSEET
                     SDKGTLLNKLRAERNLPPVKIVVVPIVLAKDGKAISTTRIKNSEIDSHGNLN"
     gene            1588..1719
                     /locus_tag="QGF01_04490"
     CDS             1588..1719
                     /locus_tag="QGF01_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20182.1"
                     /translation="MIEKLKGVGDDYLPDLLAVIASQYTKPGAKPTISGMKTPIILE"
     gene            complement(1818..1891)
                     /locus_tag="QGF01_04495"
     tRNA            complement(1818..1891)
                     /locus_tag="QGF01_04495"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1855..1857),aa:Ala,seq:tgc)
     gene            1974..2522
                     /locus_tag="QGF01_04500"
     CDS             1974..2522
                     /locus_tag="QGF01_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HJN20183.1"
                     /translation="MVLLESEVALTTGDVAPNFELVGTDDQKHSLDDYKDYKGLLVVF
                     MCNHCPYVKAKIEAIKEIHEKFSHNIALIGINSNDSIKYPDDNFESMKQVVEEKDLKF
                     DYLMDEDQETAKKYGAVCTPDPFLFDQNRKLIFHGRIDDAMNPDAIAVEKTMIVNIEK
                     FLNGDTIEKDFEPSIGCSIKWK"
     gene            2562..2636
                     /locus_tag="QGF01_04505"
     tRNA            2562..2636
                     /locus_tag="QGF01_04505"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2597..2599,aa:Ile,seq:gat)
     gene            complement(2651..3547)
                     /locus_tag="QGF01_04510"
     CDS             complement(2651..3547)
                     /locus_tag="QGF01_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HJN20184.1"
                     /translation="MTRGYKIEDIKEKILDLLANSKTGFSGLEISGRLGINRITVSKY
                     LNIFSAEGLIKQKNIGNVIIWFVEDGIEKFHLPEDFFKARTRYSEYLISKRENLVYTL
                     IRNCFHSIENPIKIITEIIVPGIVYTQTLFDQGKIGKSELTFMEKMISNSIQIINLEN
                     VETDQKKNVIVISADSKSTLFSDAISTSFHSDGWQVYSLGDMSSSIDVLFDLDLQKFL
                     TKVWKRKMGIMIIVIFSATEEGLQFFAESVKSIRGKSSKKRLYLVLCGKLEKIKIDAD
                     FIDEDLETVLQWSQATFERSVS"
     gene            3654..4040
                     /locus_tag="QGF01_04515"
     CDS             3654..4040
                     /locus_tag="QGF01_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20185.1"
                     /translation="MPKPGFKSITISESVYDKFNQAYLKNKSELTMKGVNSFAGYVTY
                     LLEDVMKKDKTFARYAPKLEKVSVDSDRIILKDNMKNRIAEVAIQNGELFCLLCDEKD
                     CVHIGYVFALPDVYEILNSKGIKQPK"
     gene            complement(4046..4167)
                     /locus_tag="QGF01_04520"
     tRNA            complement(join(4046..4090,4107..4120,4142..4167))
                     /locus_tag="QGF01_04520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4108..4110),aa:Leu,seq:taa)
     gene            4259..4332
                     /locus_tag="QGF01_04525"
     tRNA            4259..4332
                     /locus_tag="QGF01_04525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4293..4295,aa:Thr,seq:ggt)
     gene            complement(4333..>4774)
                     /locus_tag="QGF01_04530"
     CDS             complement(4333..>4774)
                     /locus_tag="QGF01_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HJN20186.1"
                     /translation="DLGSITHENRLYADLNSKDNLLTVSRILQSSNIEFFCRDFEAIL
                     HDTKKGDFVYLDPPYQPVSKTANFTSYTTRNFTDDDLERLATIAEKLHFKGCKILISN
                     SKSKKVQNSFSSSIWKMKEIHVNRAINSNSKKRTGHSEILIKNY"
BASE COUNT         1587 a          781 c          810 g         1596 t
ORIGIN      
        1 cattaattca taatattatg ataccggcac ttggtggtcc caaaggaaaa atgtctgcat
       61 cgaatgaaaa tgaaacaata tacaccacag attctcctga ggttgtaaga aaaaagatta
      121 acaaatatgc attttctgga ggacaaccag atattgagga gcacagaaag aaaggcggca
      181 atccagatat tgatgtttca tatcaatacc tcagaatctt ttttgaacct gatgacaaaa
      241 aacttgaaca gattcatgat gattataaat caggtaagat gcttactggt gaactgaagg
      301 aaattctagt tgaaaagatc aaccagtttt tggcttctca tcaacaaaag cgtgaaaagg
      361 caaaaaatga actggataag tttctactaa aagattgaac gaaatactaa ttctctgtga
      421 agataaatat gaagcacaga aactttctgg tttcatattt agtaaagatg aaaaagaatc
      481 attcatctca gaaattttga atgttgtggg taatgaaatc gttatatcat taaaagataa
      541 atccgcccat agtgttctac tcaaagatga tcatcaggtg gaagcatttg ttgattttat
      601 ccaatctgtc atagaaaagg agcataaagt attgaaactg gatattttag aaaactctgt
      661 taaattgact aagggatgaa ttcaaactag tgatttatcc atttcagaac ttagtatttc
      721 ttcaacttta agaaagtaaa gttttgttga ataaggcata ctgtctaaat ttgcgtcaac
      781 aatctgcata aagtaacgaa ctgcacgttt tgaaatatca atatcaaact tcaatgggag
      841 aattggatcc tgtttcacat tgatcttatc tgtcaaccat ggtggagcca tctctcttgt
      901 ttcatttatt attttttcgt accaatagga gatattttca ggatttaacc catcctttag
      961 atttactaca tcattattga tctgatttag tattttactg gtaacactca tacaaagaat
     1021 tcataaaatt tttgttttat tcgggttact caaagataga ggagctaatt gtcaattcaa
     1081 atttccatgt gaatcgattt ctgaattttt gattctggtg gttgatatgg ctttgccatc
     1141 tttggctaat actataggca ccacaactat ttttaccgga ggtaggtttc tttctgccct
     1201 caatttgtta aggagtgtcc ctttgtcgct agtttcctca cttacaatca gggccttcac
     1261 gtattcctca agaaccgctg gaccaaattc attctcgagt cttgctatct catatgccga
     1321 atctggaaag ttttcttcaa ttgttgattt taacagtgat attctttttg aataatcatt
     1381 tcctggtttt ttcccatttt ttactgctaa ttgatcacta gtgacaccga ttattacttt
     1441 ggttgaaatt gaaaatgctt tattcaataa tgccatatga ccactatgaa tgatatcaaa
     1501 tgttccaccc atagcaacta gatcgaacaa tttcatgtaa aattatgtgt ttaatagatt
     1561 ataatttttt ataaaataaa tgaaaacatg atagagaaat taaaaggagt gggtgatgat
     1621 tacttgccag atcttctagc agtaattgca agccagtata ccaaacctgg tgccaaacct
     1681 accatcagtg gtatgaaaac accgatcata ttagaataat ctgtcatatt caaaataatt
     1741 aattattcat atttaaatcg ttctcaaatc tccaaaattg taagtgatct acagactaga
     1801 aaataacaaa taaaagatgg actgaagggg atttgaaccc ctgacccctc gcgtgcaagg
     1861 cgagtattct accagtctga actatcagcc ctcttctaag atcaagccgt gtagatttta
     1921 attgttgtct ttaatttggt aagaatttta ttggatccat cataactttt tttatggtac
     1981 ttctagaatc tgaggttgca ttaacaactg gagatgtggc acctaacttt gaactggtgg
     2041 gtactgatga ccaaaaacat tcacttgatg actataaaga ttacaaggga ttgcttgtgg
     2101 ttttcatgtg taatcactgt ccatatgtca aagcaaaaat agaggcgata aaggaaatac
     2161 atgaaaaatt cagtcataat atagcattaa tcggcatcaa cagtaatgat tctatcaagt
     2221 atcctgatga taattttgag agtatgaagc aagttgtaga ggaaaaagac ctgaaatttg
     2281 attatcttat ggatgaggat caagaaaccg caaaaaaata tggtgcagtt tgtacacctg
     2341 atccatttct ttttgatcag aatagaaaat tgatttttca tggaagaatt gatgatgcga
     2401 tgaatccaga tgctattgcc gtcgaaaaaa ctatgatcgt caatattgaa aaatttctaa
     2461 atggagatac gattgaaaag gattttgaac cgtcaatagg ctgttcaatc aaatggaaat
     2521 aacagcagac ttaaatttcc acagagctcg attgattttc agggctcgta gctcagcttg
     2581 gctggagcgt tcgactgata atcgaaaggt catgagttcg aatctcatcg ggcccatctt
     2641 ttattttcat ttatgaaact gatctttcaa atgttgcctg tgaccattgt aaaacagttt
     2701 caagatcttc gtctataaaa tcagcatcaa tcttgatttt ttccaattta ccacataaaa
     2761 caagataaag acgcttcttc gatgatttac ctcttatgga tttcacagat tctgcaaaaa
     2821 attgcaatcc ttcctctgtt gccgaaaata tgactattat cattattccc atctttcgct
     2881 tccaaacctt tgtcaaaaat ttctgtaggt caagatcaaa taaaacatca atagaggagg
     2941 acatatcgcc tagagaataa acttgccaac catctgagtg aaatgaagta gatatggcgt
     3001 ctgaaaataa tgtgctttta ctatcagcgg atataacaat cacatttttt ttttgatctg
     3061 tttcaacgtt ttctaggtta ataatttgta tagaatttga aatcattttt tccataaaag
     3121 ttagttcaga ttttccaatc tttccctgat caaaaagggt ctgagtatac acaattccag
     3181 ggactataat ctctgtaatt attttaattg ggttttctat agaatgaaag caatttctga
     3241 ttagggtata aacaagattc tccctcttag aaattaggta ttcactgtat ctagttcttg
     3301 ccttgaaaaa atcttcagga aggtgaaatt tctctattcc atcctcaaca aaccatatga
     3361 tcacattgcc aatgtttttc tgttttatca aaccctcagc agaaaaaata ttcagatatt
     3421 tactaacagt gattctgttt attcctagtc taccagaaat ctcgagaccc gaaaatcctg
     3481 tctttgaatt agccagtaaa tccaatattt tctcttttat atcttcaatt ttgtatcctc
     3541 ttgtcatatg cagtaatcag tatatcttgt ataaaggcta atttcatgaa tgaaaaatat
     3601 taataaaaaa gattatatag ttagtacctt attttacggt aaggtactat aatatgccaa
     3661 aaccaggttt caaatcaatt acaatctcag aatctgttta tgataaattt aatcaagcgt
     3721 acctgaagaa caaaagtgag ttgacaatga aaggtgtcaa cagttttgcg ggatatgtta
     3781 catatcttct cgaagatgta atgaagaaag ataaaacctt tgcacgatat gctccaaaac
     3841 tagaaaaagt ttcagttgat tctgatagaa ttattctaaa agataatatg aaaaacagaa
     3901 ttgcagaagt tgcaattcaa aatggtgaat tattctgttt attgtgtgat gaaaaagact
     3961 gtgttcatat tggatatgta tttgcacttc cagatgttta cgaaatccta aactcaaaag
     4021 gtatcaaaca accaaaataa aatagtgcag gaggtgagat tcgaactcac gaactcctga
     4081 gagacgggat cacccatttt tatgatctta agtcccgcgt gtccaaaagc agaaatgcct
     4141 tctttggcca ggcttgacta ctcctgcact aaaattagta gatttgaaac gaaatttaac
     4201 agtttatggc tgaaaaatgg tcaagaatta taagtatttt tttttggtca ttatgtatgc
     4261 tccgatagct cagcctggta gagcgttaca ttggtaatgt aaaggtcgtg ggttcagatc
     4321 ccgctcggag ctttaataat ttttaatgag gatttcagaa tgacctgttc gtttttttga
     4381 atttgaattt attgcacgat ttacatgaat ttccttcatc ttccagattg atgacgaaaa
     4441 tgagttttgg accttttttg attttgaatt tgagattaat atcttgcatc ctttgaaatg
     4501 taatttttct gcaatagttg ctaatctttc cagatcatca tcagtaaaat tcctagtggt
     4561 ataacttgta aaattagcag ttttactcac tggttgatat ggtggatcaa gatatacaaa
     4621 atcacccttc tttgtatcat gcaaaattgc ttcaaaatct ctacagaaaa attcgatatt
     4681 tgaagattgt aagattcgac ttactgtaag taaattatct tttgaattga ggtctgcata
     4741 taacctgttt tcgtgagtga tactgcccag atcg
//
LOCUS       DASCPJ010000098         4524 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001558703, whole genome shotgun sequence.
ACCESSION   DASCPJ010000098 DASCPJ010000000
VERSION     DASCPJ010000098.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001558703"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="QGF01_04535"
     CDS             complement(<1..1685)
                     /locus_tag="QGF01_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HJN20187.1"
                     /translation="MSESVFLSPKSIAVIGASDKEGSVGRAITSNIMKGYKGIVYPIS
                     PTRETVFDQKAYKSVMDVSESIDLAVVITKNTIVPIVLEECGKKGIKGAIVITAGFKE
                     VDEEGKKLEQKLKDIAAKYNVKVIGPNCLGVMNLEPTTMMNSTFLKITPKSGQIALVS
                     QSGAICAALVEDASAQGIGFSAVVSMGNKADMTEIDILKMLADHEQTKVIVMYLEDMG
                     NGQEFLKVCKQITKNNKVKKPILVLKSGRSPEGAKAAMSHTGALMGSDEIYDAVLKQS
                     GAIRVDTMEELFDYATAFSKQPLPTEGELVIVSNAGGPAIISTDSCSKLGIKMAKIEE
                     IRPKIDAVIPPWGSSRNPVDIVGDADYNRFENVLNEVLQHNNVGSVISMCTPSATLDY
                     DKLAEVIVKMSKKYKKTMLASLMGLDEGITNREILANGDVPYYTYAEGSIRALKAMLR
                     FVEWTKTPEGKITKFEVDIQRAKQVFDKVKAEGRTNLLEEEGLEILGAYGFPLPKSIL
                     ARTEDEAVEAANKIGYSVVMKIASPQIVHKSDAGGVKVNLTNDDEVRNAFKEIV"
     gene            complement(1732..2088)
                     /locus_tag="QGF01_04540"
     CDS             complement(1732..2088)
                     /locus_tag="QGF01_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HJN20188.1"
                     /translation="MITNIYVDGSGGAKSGYGFFVKETGESFFEEKPGITNNQAEYLA
                     IISALKKYEKLNGEIIIYSDSKNTVNQLNHTFAINNEQLRSLARDAWSLISKFSNLKI
                     IWIPRGENLAGKMLGS"
     gene            2182..3123
                     /locus_tag="QGF01_04545"
     CDS             2182..3123
                     /locus_tag="QGF01_04545"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HJN20189.1"
                     /translation="MIKPGKTVKDIKISNESTMHDIFNELGQSGGFESRNVADGVEIL
                     TDMIQDENCLKFLSFIGAIVSTGFRGILRDMIKKKWCDVVITTCGALDHDIARHFAEY
                     KEGSFTMDDKELADQNIHRLGNVLVPMENYGPLIEEKVQNILEQAYNAGKKEFSTADI
                     NRELGKSMGEDSFLYWAYKNNISVIVPGIMDGAVGSQVWMFAQKHPDFKLDLIEDANF
                     LSGLVFKAKKSGALMLGGGVPKHHTLWWNQYREGLDYAVYVTTAQEFDGSLSGALVRE
                     AISWGKVTKHAKETTIHAEITTVFPFMYKALLEKLEK"
     gene            3169..4275
                     /gene="speY"
                     /locus_tag="QGF01_04550"
     CDS             3169..4275
                     /gene="speY"
                     /locus_tag="QGF01_04550"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HJN20190.1"
                     /translation="MDHHNFKGKDIPHIKLNSKMNIKELVEIYANSGFNGRRLGEAAK
                     LYSKMIHENATICLTVAGALTPVGFGGIIKTLLEKGFVDWIVTTGANVYHEDHFAWGL
                     PVKQGYSNVDDNILYDKEIVRIRDVYVKFYETLEMEDQVIQKIFQNKIMDKPFTTAEF
                     CNVLGNISKKKAKYPERSFVTTAYELDVPVYVSTLKDSSLALNLAIHRLKNKTYNLDF
                     VREIIEQAGIVYNAKKSGILELGGGVPKNTAQQTGPLLDQILRKDHGGQDYIIQITDA
                     RPDTGGLSGATLEEGKSWGKVQDSHEDLITVYADATIAFPILALYAISNEKPRKPKRL
                     YKKLDSYYESLRDSAVKTPDKFAKLLKKSKINLD"
     gene            complement(4267..>4524)
                     /locus_tag="QGF01_04555"
     CDS             complement(4267..>4524)
                     /locus_tag="QGF01_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20191.1"
                     /translation="KKYVLENGQTDKSLLKSDLVKIISSLGISEVVYTVARWSLQYYL
                     LLLNYEPYMASIISQMISTVIYMFTINLIIKLTKLFKNDSV"
BASE COUNT         1478 a          783 c          767 g         1496 t
ORIGIN      
        1 acaatttctt tgaatgcatt acgaacttca tcatcatttg taagattgac tttaacacca
       61 cctgcatctg atttgtgtac tatttgtggt gaggcaatct tcataactac agaatatcca
      121 atcttatttg cagcttcaac agcttcatcc tcagtccttg caagaatact ttttgggaga
      181 ggaaatccat atgctcctaa aatttcaagg ccttcttctt caagtaggtt tgttcttccc
      241 tctgctttta ccttatcaaa tacttgtttg gctctttgaa tatcaacttc aaattttgtt
      301 atctttcctt ctggagtttt tgtccattca acaaatcgaa gcattgcctt aagtgcacga
      361 atagaacctt ccgcataggt ataatacgga acatcgccat tggcaagaat ttccctattt
      421 gtgattcctt catccaaacc catcaaactt gctagcattg ttttcttgta tttttttgac
      481 atttttacaa tcacttctgc cagtttatca taatccaatg ttgcagaagg tgtacacatt
      541 gaaattacag acccaacatt attgtgttga agaacttcgt tcagaacatt ttcaaatcta
      601 ttataatctg catcaccaac tatgtcaact gggttccttg aactacccca aggtggaata
      661 actgcatcta tttttggtct aatctcttca atctttgcca ttttgattcc aagttttgag
      721 catgaatcag ttgaaatgat tgctggaccg ccagcatttg aaacaattac aagctcacct
      781 tctgtaggaa gaggttgttt agaaaatgct gttgcataat caaacagttc ttccatagta
      841 tcaactctaa tagctcctga ttgtttgagt accgcatcat agatctcatc tgaacccatc
      901 aaagcacctg tgtgcgacat tgctgccttc gcaccctcag gacttcttcc tgatttcaat
      961 accaaaattg gttttttgac tttgttattt tttgtgattt gtttacatac tttgagaaat
     1021 tcttggccat tccccatatc ctcaagatac atcacaataa cttttgtctg ttcatgatca
     1081 gctaacattt tcaatatatc tatctcagtc atgtcagctt tgttgcccat acttacaaca
     1141 gctgaaaatc caattccttg tgcacttgca tcttcaacaa gtgcagcaca aattgcaccg
     1201 ctctgtgaca caagtgcaat ttgaccagat tttggagtta ttttaagaaa agtagaattc
     1261 atcatagttg ttggttctaa gttcattaca cccaaacaat ttggaccaat aacttttaca
     1321 ttatatttag cagcaatatc ctttaatttt tgttctagtt ttttgccttc ctcatccact
     1381 tctttaaaac cagctgtgat tacaattgca cccttgattc ctttttttcc acactcctca
     1441 agaactattg gtactatggt attctttgtg attacaactg caagatcaat cgattcagaa
     1501 acatccataa cgcttttgta tgctttttga tcaaacactg tctctctagt tgggctaata
     1561 ggatagacaa ttcccttgta acccttcata atattagacg taattgcacg accaacacta
     1621 ccttctttgt cagatgcacc aattacagct atagattttg gagaaagaaa aactgattca
     1681 gacatagcaa tgaattcact aattttctat aataaagtta ttcatgatca tctaactgcc
     1741 taacattttt cccgctaaat tttctcctcg tggaatccat ataattttga ggtttgaaaa
     1801 ttttgatatc aaagaccatg catcacgagc caaagagcgt aattgttcat tatttattgc
     1861 aaaagtatga ttaagttgat tgactgtatt ttttgaatca ctgtaaataa ttatttcacc
     1921 gttaagtttt tcatattttt ttaatgctga aattattgct aggtattctg cctgattgtt
     1981 tgttattcca ggcttttcct caaagaatga ttcacccgtt tcctttacaa agaaaccata
     2041 acctgatttt gctccacctg atccgtctac atagatattg gttatcatct ttgaataggc
     2101 tacaaatttt cttattaaat ttattgtaac tcatgttttt atgcataaga atatctatag
     2161 catattcaaa ttttatgtat aatgatcaaa cctggaaaga cagtaaaaga tattaaaatt
     2221 tctaacgaat ccactatgca tgatattttc aatgagttag gacaatcagg gggttttgaa
     2281 tcacgaaatg ttgcagatgg tgtagaaatt cttactgata tgattcagga tgaaaattgt
     2341 ttaaaatttt tgtcgtttat cggcgcaatt gtttctacag gttttagagg aattctacgc
     2401 gatatgatca aaaaaaaatg gtgtgatgtt gtgataacaa cttgcggtgc acttgatcat
     2461 gatattgcaa gacattttgc ggaatacaaa gagggttctt ttacaatgga tgataaggag
     2521 cttgcagatc aaaatattca cagactaggt aatgtattgg tgccaatgga aaattatggt
     2581 ccattaatag aggaaaaagt tcaaaatatt ctagaacaag cttacaacgc tggaaaaaaa
     2641 gaattttcta ctgcagatat taatcgtgaa ttgggaaaat caatgggtga agattcattt
     2701 ctttactggg cttataaaaa taatatctct gtcatagtcc ctggaataat ggatggtgct
     2761 gtaggaagtc aagtatggat gtttgcgcaa aaacatccag attttaaatt agatttgatt
     2821 gaggatgcaa attttttatc aggactggta ttcaaagcaa aaaaatccgg ggcattaatg
     2881 cttggaggtg gagttccaaa acaccatacg ttatggtgga atcagtaccg tgaaggattg
     2941 gattacgcag tctatgtaac taccgcacaa gagtttgatg gtagtctaag cggtgcatta
     3001 gttagagaag caatatcatg gggaaaagta accaagcatg caaaggaaac tacaatacat
     3061 gcagaaatta ctacagtgtt ccctttcatg tataaagcac ttttagaaaa actagagaag
     3121 tgaaaattca ataaaacatt aaaaatcagt gattgatatt tttcaaatat ggatcatcat
     3181 aattttaagg gtaaagatat tccacatatc aaactcaatt caaaaatgaa tatcaaggaa
     3241 ttagttgaaa tttatgcaaa ttcaggtttc aatggaagac ggttgggtga ggctgcaaaa
     3301 ctatattcaa aaatgattca tgagaatgca acaatctgtc ttactgttgc aggagcactg
     3361 acacctgttg gatttggtgg aataatcaaa acattactag aaaaaggatt tgtagactgg
     3421 attgttacca ctggtgcgaa tgtatatcat gaggatcact ttgcatgggg tcttcctgtc
     3481 aagcaaggat attctaatgt agacgacaat attttgtatg ataaggaaat tgtacgtatt
     3541 cgtgatgtat acgtaaaatt ttatgaaaca ttagaaatgg aagatcaggt cattcagaaa
     3601 atctttcaga ataaaattat ggataaacca ttcacaactg cagaattttg taatgtttta
     3661 ggaaacatca gcaaaaaaaa ggcaaaatat ccagaaagaa gtttcgttac tactgcgtac
     3721 gaacttgatg tgccagtcta tgtttcaacc ctgaaggatt catcattagc attaaatctt
     3781 gcaattcatc gtttaaagaa taaaacatac aatctagatt ttgtaagaga gataattgag
     3841 caggcgggaa ttgtttacaa tgccaagaaa tctggtattt tagaattagg aggcggtgta
     3901 ccaaaaaata cagcacaaca aactggtcca cttctagatc aaattttgag aaaggatcat
     3961 ggtggacaag attatatcat acagataaca gatgctagac ctgacacagg cggattgtct
     4021 ggcgcaacac ttgaagaagg aaaaagttgg ggaaaggtac aagactcgca tgaagattta
     4081 atcaccgtct atgcagatgc aactattgca tttcccatat tagcattata tgctattagt
     4141 aatgaaaaac caagaaaacc aaaacgccta tacaaaaaac ttgattcata ctatgaatct
     4201 cttcgagatt ctgcggtaaa aactccagac aaatttgcaa aattattaaa aaaatcaaaa
     4261 attaatttag actgaatcgt ttttgaataa ttttgttagt ttgattatca aatttatggt
     4321 aaacatatag atcacagtag aaatcatttg tgagattatt gatgccatgt atggttcata
     4381 atttaacaaa agtaagtaat actgcaggct ccatcttgca acggtataaa caacctcgct
     4441 aatacctaat gatgagatga ttttgacgag atccgatttt aataatgatt tatcggtttg
     4501 gccattttct aaaacatatt tttt
//
LOCUS       DASCPJ010000099         3024 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001560843, whole genome shotgun sequence.
ACCESSION   DASCPJ010000099 DASCPJ010000000
VERSION     DASCPJ010000099.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001560843"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="QGF01_04560"
     CDS             <1..363
                     /locus_tag="QGF01_04560"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HJN20192.1"
                     /translation="EGVDLLCYLTPSEHLALPNPEEVKEGLIAYRIAAHAGDLVKLRE
                     KSIKWDLKMTEARRTLDWDAQLALSIDPEKAVMIHSRTGQHPGNNVPCTMCGGACVYL
                     MLPQQRKYVKDNELQQVE"
     gene            complement(346..753)
                     /locus_tag="QGF01_04565"
     CDS             complement(346..753)
                     /locus_tag="QGF01_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HJN20193.1"
                     /translation="MRYTNIVTSIIKHQEKILLLKRSDKVKTMKNMWSGVSGVIENDD
                     ESPLSRAKIEIFEEIGIVEKKIELLKSNEQIKIDSPQYKNHTWCIFPFLFKVETPEIK
                     LNWENSEFVWIDPNDIVGYKTVPALKEILFDLL"
     gene            complement(756..1610)
                     /locus_tag="QGF01_04570"
     CDS             complement(756..1610)
                     /locus_tag="QGF01_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HJN20194.1"
                     /translation="MTEKITIIGAGGGMGILFTKYFTDMGFEVTGHDIDNQKKVRASV
                     KSDSLIGSILQPDYVLLCTPTKATPEIIRLISKEMKRGAFLIEISSEKSKTVPALAKT
                     PSKINPICIHPMFGPGIKKIKGRNIICIPIKDAKKELATTKKLFPGANFVTIDSTEHD
                     KKIALILGLTHLMNLAFANILARDETITLTEKMSGTTFKAQKIIAESILTETPELINT
                     IISNPELRKYGEEFWRDIGRLLADSQEGKREDVMNYVKACQEKISKNVDLNKSSKKLS
                     NMVNLIDK"
     gene            complement(1610..3004)
                     /locus_tag="QGF01_04575"
     CDS             complement(1610..3004)
                     /locus_tag="QGF01_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJN20195.1"
                     /translation="MSDIEEHRKKIEEITLEMIKLLKTRTDIAKQIGDAKASLGMTVT
                     DEEREDALRSQVAKICKEIDLDQSTASKFLNLLLNESVKVQSDNKQTHLSMFLKAKAL
                     EEEGKKIIHLEVGEPDFKSPKEVKTALAEVYDKGYGKYGSPKGITELRKALAEDRGTH
                     TSWSGTITEDNIMICPGARFGVYLAITTLLNPGDEIIVIEPAWPAYKDCALNAGIKVR
                     TIKTTLETKWEPSIEQINNVINENTKMIVLNYPNNPTGKILPKKLLDNIIQTAKEHDL
                     YVLSDEIYEYYAMEESVGLQLWEYRYEKSIIISSFSKSYAMTGYRIGWVLTEPSIIEK
                     MSKLQALSLTNVSEPIQYVALKAWTNTRTGSSYVADNADLVRSRLEALVKTAKDIGLE
                     FIEPEGAMYLFAKTKYKNFDATKFSEKLLEHGVAIAPGEGFGDYKEFFRITAIDETKL
                     KEGMTILDSMLKEL"
BASE COUNT          823 a          572 c          488 g         1141 t
ORIGIN      
        1 gagggggtgg atcttttatg ctatctaact ccatctgaac atttagcact accaaaccct
       61 gaagaggtga aagagggact tatagcgtat agaatagccg cacatgcagg cgatttggtg
      121 aaactccgtg aaaaatccat caaatgggat ttaaaaatga ccgaagctag aagaacactt
      181 gattgggatg cacagttggc actttctatt gatccagaga aagctgtaat gattcattca
      241 agaactgggc agcatcctgg gaataacgtt ccatgtacta tgtgtggcgg tgcatgtgtg
      301 tatcttatgc ttcctcagca gagaaaatat gtaaaagata atgaattaca gcaggtcgaa
      361 taatatttcc tttaatgctg gaacagtttt gtagccaact atatcatttg gatctatcca
      421 tacaaattct gaattttccc agttgagttt aatttctggc gtttcaacct taaagagaaa
      481 tgggaaaata caccatgtat gatttttgta ttgcggcgaa tcaattttta tttgctcatt
      541 agatttcaaa agttctattt ttttttccac aatacctatt tcctcaaaaa tttcaatttt
      601 tgctctagat aatggtgatt catcatcatt ctcaattaca ccactaactc cagaccacat
      661 atttttcatt gtttttactt tatcacttct ttttaaaagt aaaatttttt cttgatgctt
      721 aattatagaa gtaactatgt tcgtgtatcg catttttatt tatcaatcaa attaaccata
      781 tttgataatt ttttagatga tttattcaaa tctacatttt tagatatttt ttcttggcat
      841 gctttcacat aattcataac atcttcacgt tttccttctt gagaatctgc taatagcctt
      901 cctatatctc tccaaaactc ttcaccatat tttcttagtt ctggatttga aattattgtg
      961 ttgatcaact ctggtgtttc agtaagtatg ctttcagcga taattttctg agctttaaat
     1021 gtagtacctg acattttttc agttaatgtt attgtttcat ctcttgcaag tatatttgca
     1081 aatgctaaat tcatcaaatg ggttaaccca agaatcaatg ctattttttt atcatgttca
     1141 gtagaatcaa ttgtaacaaa atttgcaccg gggaaaagtt tctttgttgt tgccaattct
     1201 ttttttgcat ctttaattgg tatacaaatt atattacgtc ccttgatttt ttttattcct
     1261 ggtccaaaca ttggatgtat acagatagga ttaattttag atggtgtttt tgcaagagct
     1321 ggaacagttt ttgatttttc agatgatatt tctatcaaaa atgcaccacg tttcatttcc
     1381 tttgaaatta acctaataat ttctggtgta gcctttgtag gcgtacatag caaaacataa
     1441 tcaggttgta aaatcgatcc aatgagagag tcagatttaa cagaagcacg tacttttttc
     1501 tgattatcaa tatcatgtcc tgtcacttca aatcccatat cagtaaaata ttttgtaaat
     1561 agtataccca tgccgccacc cgcgccaatt attgttattt tttcagtcat cacaattcct
     1621 tcaacatact atctaatatt gtcatgcctt cctttaactt agtttcatca atggctgtta
     1681 tcctaaaaaa ttccttatag tctccaaatc cctcgcctgg agctatggca acgccatgtt
     1741 ctaatagctt ttctgaaaat tttgtcgcgt caaaattctt gtattttgtt ttagcaaaca
     1801 ggtacattgc tccttcaggt tcgataaatt cgagaccgat atcttttgca gttttgacta
     1861 gtgcttctag tcttgatctt acaagatctg cattgtcagc aacatatgat gaacctgtcc
     1921 tagtgtttgt ccaagctttt aatgcaacat attgaattgg ttctgagaca tttgtaagac
     1981 taagtgcttg taactttgac attttttcta taatggatgg ctctgtaagt acccagccaa
     2041 tcctatagcc agtcatggca taagatttag aaaacgagct tatgattatg gatttttcat
     2101 atctatattc ccaaagctgc agtccaacac tttcttccat ggcgtaatat tcgtatatct
     2161 cgtcactaag tacatagaga tcatgttcct ttgctgtttg gataatatta tctaggagtt
     2221 tttttgggag aatctttccc gttggattgt ttggatagtt tagcacaatc atttttgtat
     2281 tttcatttat tacattgttg atttgttcaa tagacggttc ccattttgtt tctaaggttg
     2341 tcttgattgt tcttactttg atgccagcat ttaatgcaca gtctttgtat gccggccatg
     2401 caggttctat tacaattatt tcatcaccag gatttagtag tgtagttatc gccaagtaaa
     2461 ctccgaatct tgcaccaggg caaatcatta tgttatcttc agtaattgtg ccactccacg
     2521 aggtgtgggt tcctctatcc tcagccaaag ccttccgcaa ttctgtaatt ccctttggag
     2581 aaccatactt tccatatcct ttgtcgtata cttctgcaag cgcagttttc acttcttttg
     2641 gtgacttgaa atctggctca ccaacttcaa gatgaattat ttttttacct tcctcttcaa
     2701 gcgcttttgc tttcaaaaac attgaaagat gtgtttgctt gttgtcagac tgtaccttta
     2761 cagattcgtt aagcagtaaa ttgagaaatt ttgatgccgt ggattgatcc aaatcaattt
     2821 ccttgcatat cttggcaact tggcttcgta acgcatcctc tctttcctca tctgttactg
     2881 tcatgccaag acttgctttg gcatcgccta tctgcttggc aatgtctgtt cttgtcttca
     2941 acaacttgat catctcaagc gttatctctt caattttctt acgatgttcc tctatatctg
     3001 acattttaga taactggttt tatg
//
LOCUS       DASCPJ010000100         6055 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001572495, whole genome shotgun sequence.
ACCESSION   DASCPJ010000100 DASCPJ010000000
VERSION     DASCPJ010000100.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001572495"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(373..1236)
                     /locus_tag="QGF01_04580"
     CDS             complement(373..1236)
                     /locus_tag="QGF01_04580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJN20196.1"
                     /translation="MSSELNYTVVLGVYNEEERLELALRNFFGKAEIIIIDNFSKDKT
                     VEIAKKYTDQVYSYKNPGNQDKGYYKFALSLVKTDYVYFASAAEIVPIALLKEFHRIS
                     KENTKYKAIAFMRKSISYGVWTHRNWSNHETIKRNARFIRKECIDIEAHEIHSEIKNN
                     IPKSEVLLLPANDNHVVWQFRDYDVTTTELKHSRYSIAESRELFESGKRTGVFSLLFY
                     SLKEFYSSYVRNRGYKAGMIGFFTAIWRSQMKFNVHARLWEMQNKLTLNDVKIIHENM
                     KKEMLDKIEIV"
     gene            complement(1249..2352)
                     /locus_tag="QGF01_04585"
     CDS             complement(1249..2352)
                     /locus_tag="QGF01_04585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HJN20197.1"
                     /translation="MTIDKMILTAGPSITEKEINYVTDAVTNGWNNNWNSYLSRFEDA
                     FSKYLNVSHATTTSSCTGALHLGLLACGIGKGDEVIVPEITWVATASAVAYTGATPVF
                     SDIDEGSWCLNLQSVESLITSRTKAILPVHLYGHPANMPKVMALAEKYNLYVIEDAAP
                     SIGAEVAGKKTGSFGHISGFSFQGAKILVTGEGGMLVSNDKTIFDKAKFLGDHGRDPS
                     KPLAAIEIGYKYKMSNIQAALGLAQIERANELIERKRTINARYRDNLENLDRVEVSCE
                     LPGCKSNNWMTSIEIIEADNEMREQVIKSLKENNVDSRPVFSPMSSLPMFTRNEDNPV
                     AYRIGKSALNIPSGHNLTQDQIDHVCDVLIQIL"
     gene            complement(2358..2999)
                     /locus_tag="QGF01_04590"
     CDS             complement(2358..2999)
                     /locus_tag="QGF01_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024698.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJN20198.1"
                     /translation="MPICDFEKEIEKIILFEKEKTFMECKYFSPLHDGVYITPVTLDS
                     YSSELIQNLARWRSENQNGFVKIFNVTIRGTKKWLDLAVQQRKDRLLFLVVDGNEKVI
                     GHLGVSSFDFKNKTCEVDNVVRGEISKQNEVMYSASKTLIKWIDENIKTIKIQIRVLN
                     DNTAAISLYHRLGFIPFKLEGLKKVENYEGIEWLPITTGKIDRFFLKMELKRI"
     gene            complement(2999..3904)
                     /locus_tag="QGF01_04595"
     CDS             complement(2999..3904)
                     /locus_tag="QGF01_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014798.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HJN20199.1"
                     /translation="MLEALYDLASSNHNVMLLTADLGYGVFEKFEEMYSDQFLNVGVA
                     EQNMMGVASGLALEGKVIFTYSIGNFPTLRCLEQIRNDACYHDLNINIIASGGGYSYG
                     SLGMSHHATEDLSFMRSLPGITIVAPCTAWETKNAVTCLAAQQNVGYLRIDKTKANSN
                     IRNDPFELGKSITLRQGSDITIVATGGIAEEAMKASDVLINDNIDCRVLSMHTVKPID
                     IDGIISACKETKGIISIEENNIIGGLGSAITEVCMDYGVIPKYFHRFGIKDTYTSIVG
                     SQTYLRGKSGILSDNLVQFIRKCFL"
     gene            complement(3913..4734)
                     /locus_tag="QGF01_04600"
     CDS             complement(3913..4734)
                     /locus_tag="QGF01_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HJN20200.1"
                     /translation="MSYLNLRSKELSKNIRLNAVRMVNRGGSSHIGSVLSIADILAVL
                     YANILKYQPKNPKWSERDRFILSKGHAGAGVYAVLAECGFLNKAKLMDHCQDGSVFSG
                     HVSHKGIPGVELSTGALGHGLPIGAGMALAAKLDRKKHHIYVLMSDGELDEGSNWEAF
                     LFSAHHELSNLIAVIDRNRLQSIKGTETTMALEPLQDKFLSFGWNVEEVDGHNHQELK
                     ECLNLDRRKKPTVVIANTIKGKGVSFMENEVTWHYKTPANEFFQQAIHELESIEI"
     gene            complement(4737..6050)
                     /gene="rfbH"
                     /locus_tag="QGF01_04605"
     CDS             complement(4737..6050)
                     /gene="rfbH"
                     /locus_tag="QGF01_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019082567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HJN20201.1"
                     /translation="MSQEQLRHEILELVSQFATEKYKQIEFKAGETIIPPSGKVLGEK
                     ELQFMVDASLDGWLTAGRYNEQFGNSIGSYVGVKHVLTTNSGSSANLLALTALTSHKL
                     ESRALKPGDEIITTAAGFPTTVNPIVQNGLIPVFIDVKIPTYNIDESEIEAAISEKTK
                     AIMIAHTLGNPFDLSEVKRVADKYNLWLIEDCCDALGTTYNGKMVGSFGDLATVSFYP
                     AHHITMGEGGAVFTQSGKLKRIVESFRDWGRDCYCAPGCDNTCKRRFEQQFGDLPKGY
                     DHKYTYSHLGYNLKISDMQAACGLAQMESLDGFVKSRKENFNTLKEGLVSCEDFIILP
                     EATENSDPSWFGFPITLKENSGIQRVDLLKFLDEHNIGTRLLFAGNLTRQPYFKNVNY
                     RIQGKLDRTDRIMNQTFWIGVYPGLTNEMLKEVINKIETFFGVKN"
BASE COUNT         1873 a         1244 c          849 g         2089 t
ORIGIN      
        1 aataatactg gccagcccca tccaatgacc ccagacagca tatttcgaat attctgatta
       61 ttcataaatg gtaatctttt actgctacta agattaattt tttgttaagc atatgttggt
      121 cacaaaatga tagataaaat tcacaaaaat ggtcaaggta tgcagtattt aaaacactcg
      181 aaattgctat ttctttcgat aaaagagaag ttttgagtat ctaaaaaata atcaaatatg
      241 tttaattttg attcatgcaa atccattaaa tacatcaaaa aaggatattc ttatatgctt
      301 cctctcatta attgtcaatg aggcacatat actgatatcc aaaaactccc aactattaac
      361 accccataaa tgttaaacta tttctatttt atctaacatt tcctttttca tattttcatg
      421 aattatttta acatcattta gagttaattt attttgcatt tcccataaac gagcatgcac
      481 attaaacttc atttgagatc tccatatagc tgtgaaaaag cctatcatcc cagccttgta
      541 accgcggttc cttacgtaag aactataaaa ttccttcaaa ctataaaata acaaactaaa
      601 tacccctgtt cgctttcctg attcaaaaag ctccctcgat tctgctatgc tatatcttga
      661 atgttttaac tcagtcgttg taacgtcata atctcgaaac tgccatacaa catgattgtc
      721 atttgctgga agtaataaaa cttctgattt tggtatatta tttttaattt cactatgaat
      781 ttcatgtgcc tcaatatcaa tgcattcttt tcttataaac cttgcatttc tttttatggt
      841 ctcgtgattt gaccaatttc ggtgagtcca aacgccatat gagatgcttt ttctcatgaa
      901 tgcaatagct ttatacttcg tattttcttt agagattcta tggaattctt ttaataaagc
      961 tataggaaca atctcagctg cagaagcaaa atagacataa tcagttttaa ctaaacttaa
     1021 agcaaatttg taataacctt tatcttgatt ccctggattt ttgtaactat atacctgatc
     1081 agtatatttt ttggcaattt caactgtttt gtctttacta aaattatcta taattataat
     1141 ttctgctttt ccaaaaaaat tacgtaaagc aagttcaagt ctttcttctt cattataaac
     1201 tcctaaaaca acggtataat ttaattctga cgacaatttc ccctcttgtt acaaaatttg
     1261 aatcaaaaca tcacaaacat gatctatctg atcttgagtt agattgtgtc cgctaggaat
     1321 atttagtgct gacttaccaa ttcgatatgc tacgggatta tcttcatttc tggtaaacat
     1381 tggaagcgaa ctcattgggc taaaaacagg tctagaatca acattatttt ctttcaaaga
     1441 ctttatgacc tgttcacgca tttcattatc tgcctctatt atttcaatcg aagtcatcca
     1501 attattactt ttacaacctg gtagctcaca agacacttcg accctatcta aattttcaag
     1561 attatcgcga tagcgggcat taatcgtccg ttttcgttcg attaactcat ttgctctttc
     1621 aatttgtgcc aagcctaaag cagcttgaat gttagacatt ttatatttat aaccgatctc
     1681 tattgcagct aaaggttttg aaggatccct tccatgatct ccaagaaatt tagctttgtc
     1741 aaaaatagtc ttgtcattac ttactaacat acccccctca ccagtaacca gtattttcgc
     1801 accttggaaa ctaaaaccag aaatatgtcc aaaactgcca gttttcttgc cagcaacttc
     1861 agcacctatc gatggagcag catcctcaat aacgtataag ttatattttt cagctaaagc
     1921 cattacctta ggcatatttg caggatgacc ataaagatgt acaggtaaaa ttgccttagt
     1981 tctggatgta atcagacttt ctactgactg taaatttaaa caccatgatc cttcatcaat
     2041 gtcagagaaa acaggagttg ctcccgtata tgctacagct gaagctgtag ctacccatgt
     2101 aatctcaggc acaataacct cgtctccttt acctattcca caagctaata gccctaaatg
     2161 caatgcacca gtgcagcttg aagttgtagt agcatgggaa acatttaaat attttgaaaa
     2221 tgcatcttca aatctgctca aatatgaatt ccaattattg ttccagccat ttgtaactgc
     2281 atcagttaca taattaattt ctttttctgt gatacttggg cctgctgtca aaatcatctt
     2341 atcaattgtc atatcagcta aatccttttt aattccattt tcaaaaaaaa tcgatcaatt
     2401 ttgccagtgg ttattggtag ccattcaata ccttcataat tttcaacttt tttcaatcct
     2461 tctaatttaa aaggtataaa accaagccga tgatatagag atatagcagc agtattatca
     2521 ttcaaaactc taatttgaat ttttattgtt tttatatttt catctatcca tttaattagt
     2581 gtttttgatg ctgaatacat tacttcattc tgtttgctta tttcacccct gactacattg
     2641 tcaacttcac aagtcttatt tttaaaatca aatgatgaaa cacctaaatg tccgatcact
     2701 ttttcattac catcaacaac taaaaataat agtctatctt ttcgttgctg aacagcaagg
     2761 tccaaccatt ttttagttcc tcggattgtt acattaaata tttttacaaa accattttga
     2821 ttttctgaac gccatctagc tagattttgt atcagttcag agctataaga atctaatgta
     2881 acgggtgtaa tatagacacc atcatgtaac ggtgaaaaat acttacactc cataaaggtt
     2941 ttttcttttt caaaaagtat tattttttct atttcttttt caaagtcaca tataggcatt
     3001 ataaaaaaca tttccgaatg aattggacca aattatcaga taaaatacct gattttcctc
     3061 taagataggt ttgagatcca acaattgatg tataagtgtc ttttattcca aaacgatgga
     3121 aatattttgg aatcactcca tagtccatac acacttcagt tattgcgctc cctaaaccac
     3181 ctattatatt gttttcttca attgaaataa tgcctttcgt ttctttacaa gcagaaataa
     3241 ttccatctat gtcaataggt ttgactgtat gcatacttaa aactctacaa tcaatgttat
     3301 cattaataag tacatcagat gccttcattg cttcttctgc tataccacct gtagctacaa
     3361 ttgtaatatc actaccttgt cttagagtga ttgatttccc caattcaaaa ggatcatttc
     3421 taatattgga atttgctttc gttttatcga ttcttaagta ccccacgttt tgctgagctg
     3481 ctagacatgt aacggcgttt tttgtttccc atgctgtgca gggggcaaca attgttattc
     3541 ctggtaatga cctcataaaa gaaagatctt ctgtagcatg gtgagacatt ccaagactgc
     3601 cataactata accacctcct gatgcaataa tgttaatatt tagatcatgg taacaggcat
     3661 cattacgaat ttgctcaaga cagcgaagtg taggaaaatt tccaattgaa tatgtaaata
     3721 ttacttttcc ctccaacgct aagcctgagg caacacccat catgttttgc tcggcaacac
     3781 cgacattaag aaactggtca ctatacattt cctcaaattt ttcgaacacc ccatatccca
     3841 agtcggccgt cagtaacata acattatgat tagatgaagc tagatcatat aaggcttcca
     3901 acattgcatc tctcatatct caatactttc taactcatga atagcttgtt ggaaaaattc
     3961 atttgctggt gttttataat gccacgtgac ctcattctcc ataaaagaaa ctccttttcc
     4021 ttttatggta ttcgcaatga caacagtagg tttctttcta cgatctaaat tcaagcattc
     4081 ttttaattcc tggtgattgt gcccatccac ctcttctaca ttccagccaa aagatagaaa
     4141 tttatcttgt aatggctcta acgccattgt tgtttcggtg ccttttatac tttgaagtcg
     4201 atttcgatct atgaccgcaa tgagattact taattcatga tgcgcagaaa aaaggaatgc
     4261 ctcccaattt gatccttcat caagttcccc atcactcatt aacacataaa tgtgatgttt
     4321 cttgcgatca agttttgcag ctaatgccat acctgctcct ataggcagcc catggccaag
     4381 agcgccggtt gacagttcta cgcctggaat gcccttatgt gacacatgtc cactaaaaac
     4441 tgagccatcc tgacaatggt ccattagttt agctttattt aggaaaccac attcagcaag
     4501 cactgcatat acccccgcgc ctgcatgtcc tttacttaat ataaagcgat cccgttcact
     4561 ccatttgggg tttttgggtt gatattttaa tatattagca tacagaacag caagtatatc
     4621 ggcaattgag agaacagacc caatatgaga gctaccacca cgatttacca tacgaacagc
     4681 atttaatcta atatttttgg atagctcctt tgaacgtaaa tttaaatagg acataattaa
     4741 tttttcacac caaaaaaagt ttcaatcttg tttattactt ctttcaacat ttcattcgtc
     4801 aaaccaggat aaactccaat ccaaaatgtc tgattcatga ttctatcagt cctatccaat
     4861 ttcccttgga ttctataatt aacatttttg aaatatggtt gtcttgttaa attccctgca
     4921 aacagtagac gtgtgccaat attatgttca tctaagaatt tcaacaaatc aacacgctga
     4981 ataccactat tttctttcaa tgttattggg aatccaaacc atgatggatc ggagttttca
     5041 gttgcttcag gtaatattat aaaatcttca catgaaacta aaccttcttt aagtgtgtta
     5101 aaattttctt ttcgactctt aacaaatccg tctaaactct ccatctgtgc taatccacat
     5161 gctgcctgca tatcagaaat cttaagattg tagcccagat gtgagtatgt atatttatga
     5221 tcataccctt taggtaaatc cccgaattgc tgttcaaacc gccttttgca agtgttatca
     5281 cacccaggag cacaataaca atctcttccc cagtctctga aggattcaac aatccttttc
     5341 agttttccag attgagtaaa taccgctcct ccctccccca ttgttatatg atgtgcagga
     5401 taaaaactaa ctgtagcgag atctccaaac gagcctacca tctttccatt ataagtagtc
     5461 cctaaagcat cacaacaatc ctctattaac cataggttgt acttatctgc aacacgtttt
     5521 acttctgata gatcgaaagg attgccaagt gtatgtgcga tcataatagc tttagttttt
     5581 tctgatatgg cagcttcaat ttcagattca tcaatattat aagtagggat cttcacatca
     5641 ataaatactg ggattaatcc attctgaacg ataggattaa cagttgtagg aaagcctgca
     5701 gctgttgtaa ttatttcatc tccaggcttc aaagcacgac tttctaattt atgtgaagtc
     5761 aacgcagtca aagcgagaag atttgctgaa gaacctgaat ttgtcgttaa aacatgtttc
     5821 acacccacat aactgcctat actatttcca aattgttcat tatatctccc agcagttaac
     5881 cacccatcca acgaggcatc aaccataaat tgcagttcct tttcgccaag tacttttcca
     5941 gaaggtggaa taattgtttc gccggctttg aattcaattt gtttgtattt ttctgtggca
     6001 aattgtgata ctaattctaa aatttcatgt cttaattgtt cttgtgacat attat
//
LOCUS       DASCPJ010000101         4172 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001594137, whole genome shotgun sequence.
ACCESSION   DASCPJ010000101 DASCPJ010000000
VERSION     DASCPJ010000101.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001594137"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="QGF01_04610"
     CDS             complement(<1..1724)
                     /locus_tag="QGF01_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HJN20202.1"
                     /translation="MVHITKVDIFGFKSFGFKNTTVNFEPGLVSISGPNGSGKSNILD
                     AIIFAMGENKPSIMRAPKLRALIHDIEGNRRGPKLTRVKVQFDNSDRKIPVPSDTVSI
                     SREMSEHGESVYHLDNKKMNRTQILDLLDMADAGLNQLNAVQQGTVTRISEFSNEEKR
                     KTIEDLIGLSYFDEKKNQSLKQLTDADQKLEVAMAKMGEVKKQIDELEIERNLKLRYD
                     LLGRELDRYRALDAASKLKIIKDDKSSKEEIFNSNTSELKKLGEERSILKNEITKLRE
                     EKSEFLKDFNAYNEAKQTIDTKLSSATREFDESDSKIKTSDNRILQIEKRQTEISDEL
                     ELMSKDEGVVDSKIESIKNSVRKTTQEKNSIKKKIEDTDSALKLVLDHQSEISAQKNK
                     FDKKIQELKDQLHNVTSEKSNTITNSNNIQNKIDNNTKKHKIRIEELTNLQKHSEQLS
                     KVVFNRQSSSSQIKLQISNLSNQRIKIERDISELESILEKSSLAANRYNEKIKLVKGV
                     LHEDYTISQLKNYSEKLGIDGLVYEILSWSKQYERAVLAVSSDWVKAIIVPDLETLTS
                     LAHVVRSK"
     gene            complement(1816..2913)
                     /locus_tag="QGF01_04615"
     CDS             complement(1816..2913)
                     /locus_tag="QGF01_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HJN20203.1"
                     /translation="MVEKIKVALVGIGNCFSGLIQGIEYYKQNPSQEVIGIIHEKLRD
                     YTIHDIDFVAGFDVGENKIGKSLNEAIYEYPNMVDWIPKDTMPKTDAKIYESPALDGV
                     GIWVENRVKPIQSGKKIEELEKEIKDVLKQTGTEIIVSYLPVGSEQATKFWAQVCLDT
                     NTAFVNCMPAFIASNKEWAQKFTEKNVPVVGDDIKGQVGATIVHRTLARLCNDRGTKI
                     EKTYQINVGGNTDFLNMKEQERLVSKRISKTESVQSQLDVPLDDDKIYVGPSDFIPFL
                     ANTKLMFMRIEGRQWANIPYNMEVRLEVDDKANSAGIVIDAVRLAKIALDRGLGGPII
                     PASAYLMKHPIEQMSDPDAKTACERFVAGDD"
     gene            3017..3724
                     /locus_tag="QGF01_04620"
     CDS             3017..3724
                     /locus_tag="QGF01_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HJN20204.1"
                     /translation="MVSDFKIKETADVNIEGGFIINGFPSTGLTSAIATELMINTSRF
                     EFAATIDSDSFPPISIIKNGAPNYPTRIFVNNDLKTAIFSSYVTLDVSLHKDAAKMIL
                     KWATEKKCSTIISCVTVKTKTDSIMGVASTGTARGKLVSAGIKTVDNGAVPGIPGVLL
                     NEGVTNGQDVIVLLVGSESNAPDLRATYNLCDGISKLIPGISCNLSLLEKQAKQIESE
                     MKQVETERKDLSTSMYG"
     gene            complement(3761..>4172)
                     /locus_tag="QGF01_04625"
     CDS             complement(3761..>4172)
                     /locus_tag="QGF01_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HJN20205.1"
                     /translation="EAPSEPEAPSEPEAPSEPEAPSEPEAPSEPEAPSEPKVEQSTNG
                     TRNVIFIGTKTIMNYVTATLTQLASVPVVTIIARGKRITQAIDVSQMIVKRMNEVGFK
                     ITDVRVLSDSLKSKDGQTRSVSVIQIDIQNMLDK"
BASE COUNT         1195 a          753 c          665 g         1559 t
ORIGIN      
        1 ttttttgagc gtactacatg agcaagacta gtcaatgtct ctaagtctgg gacaattata
       61 gctttaaccc aatcagaact aacagctaat actgcacgct catattgttt actccaagaa
      121 agtatttcat aaaccagacc atctatacct aatttttctg aataattttt tagttgtgaa
      181 atagtatagt cttcatgtaa tactcccttt acaagtttga tcttttcatt ataacgatta
      241 gctgcaaggg atgatttttc taaaattgat tctaattcag atatatcacg ttcaattttt
      301 attctttgat tagaaagatt agaaatttgt aattttattt gtgatgatga cgattgccga
      361 ttaaatacta cctttgataa ttgttctgag tgtttttgaa ggtttgttaa ttcctctatc
      421 ctaatcttat gttttttagt attattatca attttatttt gtatgttgtt actgttagtg
      481 attgtatttg atttttctga tgtcacgtta tgtagttgat ctttcaactc ttgaattttt
      541 ttatcgaact tatttttttg tgcagatatt tctgattggt gatctaaaac taattttagt
      601 gcagaatcag tatcttcaat ttttttcttt atgctgtttt tttcctgagt tgttttcctg
      661 acagaattct taatggattc aatctttgaa tctacaacac cttcatcttt gctcattaat
      721 tcaagttcgt ctgaaatctc agtttggcgt ttttcaattt gtaaaatccg attgtctgaa
      781 gtttttattt tgctgtcaga ctcatcgaac tcacgtgttg cagaacttaa tttggtatca
      841 atagtttgtt tagcttcatt atatgcattg aaatctttta aaaattctga tttttcttca
      901 cgtaatttgg taatttcatt ttttagtata gatcgctctt ctcctaattt tttcagttca
      961 gatgtattgc tgttgaaaat ttcctccttt gaagatttat catcttttat tattttgagt
     1021 tttgatgcag cgtctagggc tctatatcga tctaattccc taccaagcaa atcatatcgt
     1081 agttttagat tcctctcaat ttctaattca tctatctgtt ttttgacctc gcccatcttt
     1141 gccattgcta cttctaattt ctgatctgca tcagtgagtt gttttaatga ctggttcttt
     1201 ttctcatcaa aatatgaaag tccgattaaa tcttcaattg tctttctttt ttcctcattt
     1261 gagaattcag atatacgtgt aacagttccc tgctgaacgg catttaactg gttcagccca
     1321 gcatcagcca tgtctagcaa atcgagtatt tgtgtccgat tcatcttttt gttatcaaga
     1381 tgatagacac tttctccatg ttcgctcatt tcacggctta tactcaccgt atcagaagga
     1441 acaggaattt tcctatctga attatcaaat tgaactttta cacgagtgag ttttggtcca
     1501 cgcctatttc cctctatatc atgtatcaaa gccctaagct ttggtgcacg cattatgcta
     1561 ggcttgtttt cacccattgc aaaaattatg gcgtccaaaa tgttactttt accagatcca
     1621 tttggcccag agattgaaac caatcctggt tcaaagttta ctgtggtatt tttaaaccca
     1681 aatgatttga acccaaagat atctactttt gtaatgtgaa ccaatatcta tatgatcgct
     1741 ggccctgaat tattaaacac taacaagttt agttgactat actaacaaca aatatgaatt
     1801 ttttattaat gacttttaat cgtcgccagc tacaaagcgt tcacatgcag ttttagcatc
     1861 tgggtcactc atttgttcta taggatgttt cattagatat gcacttgctg gtattattgg
     1921 tccacccaat cccctatcta gagcaatttt tgctaaccta accgcatcta ttactattcc
     1981 tgcagagttt gccttatcat caacctctag tcttacttcc atattgtatg gaatatttgc
     2041 ccattgccta ccttctattc tcatgaacat gagtttggtg ttagctaaaa atggaataaa
     2101 gtctgaagga cctacataaa ttttatcatc gtctaaaggt acatctaact gactttgtac
     2161 actttcagtt tttgatattc tcttgcttac taatctctct tgttctttca tgttgagaaa
     2221 atcagtatta cctccaacgt taatttgata tgttttttct attttcgtac cacgatcatt
     2281 acataatcta gcaagtgttc tatgaacaat ggtagcacca acttgaccct ttatatcatc
     2341 tccaactaca ggtacatttt tctcggtgaa tttctgtgcc cattctttgt tggaagcgat
     2401 gaatgcaggc atacagttta caaaagcagt atttgtgtca agacatacct gtgcccaaaa
     2461 ttttgtggct tgctccgaac ctacaggtaa atatgataca atgatttcag ttcctgtttg
     2521 ctttaaaaca tctttaattt ctttttctaa ctcttctatc tttttcccac tttgaattgg
     2581 tttaaccctg ttttctaccc atattcctac tccatctaaa gctggacttt catagatttt
     2641 ggcatccgtt tttggcatag tgtcctttgg aatccaatct accatgtttg gatattcgta
     2701 aatagcctca ttcagtgatt tacctatttt gttctcgcct acatcaaagc ctgcaacaaa
     2761 atcaatgtcg tgtatcgtat agtcacgtaa tttctcatga attattccta taacctcctg
     2821 agaaggattt tgcttataat actcgattcc ttgaatcaga ccagaaaagc agttacctat
     2881 accaactaat gcaactttta ttttttcaac cattcgatca tctgtgtgat tagagcgttt
     2941 taaggtttta cgcaacaagc gaccagacca taatcattag taaaaagata actatgatta
     3001 tagctgataa acagttatgg tttcagattt taaaataaaa gaaactgcag atgtcaatat
     3061 tgaaggtgga tttataatca atggatttcc ttctacagga ttaacaagtg ctatagcaac
     3121 tgaattaatg ataaatacat caagatttga atttgctgca actatagatt cagatagctt
     3181 tccaccaata tctataatta aaaatggtgc accaaattat ccaacaagaa tttttgtcaa
     3241 taatgatcta aaaacagcaa tcttttcatc atacgttaca cttgatgttt ctttgcacaa
     3301 agacgctgca aaaatgatat tgaaatgggc tacagaaaag aaatgctcaa caattatcag
     3361 ctgcgttaca gtaaaaacca aaacagacag cataatggga gttgcctcaa ctggaactgc
     3421 aagaggaaaa cttgtatcgg caggaattaa aacagtggat aatggagcag tgccaggaat
     3481 acctggtgtt ttattaaatg aaggggtaac aaatggtcaa gatgtgattg ttttgcttgt
     3541 tggttcagaa tctaacgctc ctgatttgag agcaacctat aatctatgtg atggtatatc
     3601 aaaattaata cctggaattt catgtaatct ttctttatta gaaaaacagg caaaacagat
     3661 agagagtgaa atgaaacagg tagaaactga acgtaaagat cttagtacat ctatgtacgg
     3721 ttaaacaaat ttactttaaa aatttataat atgtgaataa ttatttgtcc aacatatttt
     3781 gtatatcaat ttgaatgaca gacacactac gcgtttgacc atcctttgat tttaatgagt
     3841 ctgataatac acgaacatca gtaattttaa aaccaacttc attcattcgt tttacaatca
     3901 tttgagaaac atcaattgct tgtgtaatac gttttccacg cgctatgatt gtgacaacag
     3961 gtactgatgc aagttgagtc aaagtagctg tgacataatt catgattgtt tttgtgccta
     4021 tgaaaattac gttacgtgta ccatttgttg actgttccac ttttggttct gagggtgctt
     4081 ctggttctga gggtgcttct ggttctgagg gtgcttctgg ttctgagggt gcttctggtt
     4141 ctgagggtgc ttctggttct gagggtgctt ct
//
LOCUS       DASCPJ010000102        49607 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001595178, whole genome shotgun sequence.
ACCESSION   DASCPJ010000102 DASCPJ010000000
VERSION     DASCPJ010000102.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 49607)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49607)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49607
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001595178"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(182..1504)
                     /gene="glyA"
                     /locus_tag="QGF01_04630"
     CDS             complement(182..1504)
                     /gene="glyA"
                     /locus_tag="QGF01_04630"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HJN20206.1"
                     /translation="MTDSSNKEAYERIFASLEKHNQWFNDSIPLIASENVPSPAVREA
                     IISDFGNRYAEGWPGERVYAGCTYIDEVEIQCMNLAKKLFGAEFADVRPISGVVANLA
                     IYSAFSNPGDVMIAPSIPAGGHISHGKKEHSGTAGLVHGLDIEFFAFDSEEMNLDVDK
                     TKAKVEDLKSQDRLPKIAMFGGSVFLFPHPVKELADFLKGYDIHINYDAAHVAGLIAG
                     GEFQDPLREGVDTMTMSTHKTLFGPQGGLVLAFEKNAEVIKKATFPGLTSSHHIHHMA
                     AKAVTFAEALEFGKDFAAQTIKNAKALAEELNNLGFKVLGEKKGFTQSHQAVVNVLDY
                     GDGGKIEADLEKANIIVNRQLVPGDLKAKRHYMNPGGIRLGSSEVTRLGMKESDMQQI
                     ASLIKNVVIDNKDAKEVASDVLELRKNFQKTQYCFDNKLGAYEYVKLR"
     gene            1598..3295
                     /gene="thsB"
                     /locus_tag="QGF01_04635"
     CDS             1598..3295
                     /gene="thsB"
                     /locus_tag="QGF01_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HJN20207.1"
                     /translation="MAVQATQSNMPVVILKDGASQSKGLEAQKNNISAAKIIAEIVRT
                     SLGPRGMDKMLVDSLGDVTITNDGATILKEIDVQHPAAKMLVEISKTTDNEVGDGTTS
                     AVVLAGSLLEQAESLLLQEVHPTIIVDGYRKAAVKAKQYLKDIAEQVTANDKSILSKI
                     ANTSMQTKLVKRDSSQLSDMVVRSITAVAEKQGEDYTVDIDDIKVEKKAGGSISDSTI
                     IQGIVLDKEVVHSGMPKKITEAKIALINAALEIEKTETDAKINISNPQQMKSFLDEEN
                     KMLKNMVDKVVGSGATVVFCQKGVDDMAQHYLAKAGILAVRRVKESDLTKLSKATGGR
                     IVSSFDDLFEKDLGTAGLVEERKIEEDRWVFIEGCKHPKSVTLLLRAGSQRVVDEVER
                     SVHDALMVVKDVMELPSIVAGGGAPETYAATKIRNWAKSLEGREQLAAEKFADGLESI
                     ALSLSENAGMDPIDTLASLRSRQLKGDKWTGIDVIKGKVLNMKTGDVVEPLAVKNQII
                     SAATEAACMLLRIDDVIATAKSAGPPPGAEGGGMPPGMGGMGGMPPGMGMPDMGGMM"
     gene            3639..4076
                     /locus_tag="QGF01_04640"
     CDS             3639..4076
                     /locus_tag="QGF01_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20208.1"
                     /translation="MTKWKRMRRRQERKRMQEEERKQPTEQEPTLVEETPENVQTFPK
                     NESIEAVTNTPKFVISGVKYIYLIVCFALLSGIFYPLVTGDGWELSLKGIGVLAVGLF
                     GGILLYKSITSEKRRGVLVCCGFGLIAVSLIIIYELIGSPLYQ"
     gene            complement(4078..5538)
                     /gene="glnA"
                     /locus_tag="QGF01_04645"
     CDS             complement(4078..5538)
                     /gene="glnA"
                     /locus_tag="QGF01_04645"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HJN20209.1"
                     /translation="MPYKMIGGKATHVKYSPEEVFAKINQDEVSFIDLQFTGLTGRFH
                     HTTISANTFTQDQMADGLPKLDGSSIVGFASIEDSDLILKPDPSTYSLIPWASDKKTA
                     RLLCDVYWGGDRGRLEYDPRGISQNAEKFLETQGFDYSLWGPEVEFFVFDKIHWDVLT
                     PYKGQSYSIESKEAPWNQDGNGYPMGLQEGYYPSTPSDTLTPYRNECVSVLNDNFGII
                     CDNHHHEVATAGQCEIDIKYDFLTNAADGAQTYKYVVRNVAQQFGKIATMMPKPISMD
                     SGSGMHTNVSLWKNKENAFFDVDDKDELSQTGRYFCGGVINHARALTAITNPTTNSYH
                     RLVPGYEAPVYIAWSTGNRSATIRIPAHFKGEKYSYLKRLEYRVPDPSSNPYLVFPAV
                     LAAGLDGIKNKTEPGDPIKADIYKMSKSERTKHGIDVLPANLGEALDELESDKKFLES
                     IFSPKVINRIIDLERRDQKEISIRPHPHEFYLYFDV"
     gene            5599..6156
                     /locus_tag="QGF01_04650"
     CDS             5599..6156
                     /locus_tag="QGF01_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20210.1"
                     /translation="MSEQNQINVNSLHSIVLQEYENTSIQKIDSTLYSSVSRLIGNLK
                     SQEYDGHEAKIKTALVDMLTELTSSLLKTRLEKAILNNTDHSALLDEEKYILNSQEEM
                     IERKEVILSGILNGKAKLLESIVQNNKTKLIAVRFLEEVEEISGADAKKYGPYKPEDI
                     ATLPYENAQNLISKNSAVKIHWENQ"
     gene            6141..6461
                     /locus_tag="QGF01_04655"
     CDS             6141..6461
                     /locus_tag="QGF01_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20211.1"
                     /translation="MGKPIEIIIDKHRTNMSHIDVDIFEFLILTIIPVGALFIIEIIF
                     RLRKSTSWLKLTIQGITMVIFGVGYLTLITPHVLTALCLLALSGALFYQARRAKINPK
                     KSLY"
     gene            complement(6462..6689)
                     /locus_tag="QGF01_04660"
     CDS             complement(6462..6689)
                     /locus_tag="QGF01_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger domain-containing protein"
                     /protein_id="HJN20212.1"
                     /translation="MLREKCAYCGDITDMPFECNYCKDPFCSEHRLPEDHRCVKLSQI
                     RAKRFGEKGKVIRDGGTGRKNAFRKMLGRFG"
     gene            complement(6691..7182)
                     /locus_tag="QGF01_04665"
     CDS             complement(6691..7182)
                     /locus_tag="QGF01_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20213.1"
                     /translation="MEEGEKIQAHILSVWKESREFFSGRGAEIMLILTDKHLMFIKKT
                     ESKMKWWGANTQRQVVKFIQNKDVMVMIDGYTEEHLRTDLENKKNQEVSFNNILDIGV
                     KEKVWGSVLLLEILEDEQSKKYQFSIVQDWVKYPLKDPIKYMKVDWSSFVKYVKDKQL
                     VTE"
     gene            7257..10376
                     /locus_tag="QGF01_04670"
     CDS             7257..10376
                     /locus_tag="QGF01_04670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJN20214.1"
                     /translation="MNLKVLLAFAIVSALILPIAASGQVESQELDQSQSSNWDDREEL
                     KLLRVIGGPDSELFSEPTDIAVGESGNIYVADYGNKRIQILNPLGQLVQTIELKGSPH
                     GITLDKNENIYVTEWWDFIGIEKFTKTGEPATDFQIEDQSVFGLPSDIVIDPDGILYV
                     LEHRNLDIDYGENAGVHKIDTDGSYIEFIPIPETAITDKSKFSLMVMDHEGWLYIVDQ
                     SGDNIILLDSTNGNGRPLALIYFQTPTSIAINPDGYFFISDQRYENNKRSIHIFDEYY
                     LPIRTIGDAGKEEGKIWGIHGLEFDKDGNMYLLDFEQNKIQVFHIAPKVFGPLFYEET
                     EKVTPVAPSYSPSGDDLVAVLNTGSGQIVIEFFDEDAPKHVENFIALAENDWYETTLF
                     HRIIKDFMIQGGDPNTKPEPGNTSEKWGTGDPGYSIDAEFNSIKHERGIVSMARSSDP
                     NSAGSQFFIVHKDSTHLDEQYTVFGRLITSESYEILDKIANLQVNSRNQPYEGAMQQA
                     VLFDVKIVSRSSIENVLTMDPPNRILGFTPDHKYIDEELNFSVTSPVGWGIIKPAKFD
                     DNSPIYLASGPSKDGVKPQIYMNSKQLGEDGFKDFIDKRITTFHKLHDEGKLDLETEN
                     LTEFMGGHAYVVTLKQYINKDLVGNEPYQAQLKQFIFATSTTVYGITYVNLESNYPDH
                     LDLLNEFRYSFLVEGETMQTLGGVVIGDSNFDGKYYNNEEKQFRFEVPYRWEPLTVKT
                     GVGDDGVGYYRLSLEPNALHVNYFKDGVQARYMINIYETTDHAFTEHTTQMKNIYHGY
                     ENEGKLSLADEYMGMTADGHPVYFVEYLEPYYIAEDEYIPIHTREVTFANADFLYKIT
                     YTNHENNFTREISSYNYLLENFKFNFDGKMKSVDWGPNIIKYNPVDDRDPSERGGGCL
                     IATAAFGSEMSPQVQFLREIRDNTVLQTESGTLFMAGFNQFYYSFSPIIADYERENPV
                     FKEAVKLTLTPLLTSLTLLQYTSIDSESEMLGYGIGIILLNIGMYFVAPAVLIMKIRT
                     SYNKIPKNI"
     gene            10384..10710
                     /locus_tag="QGF01_04675"
     CDS             10384..10710
                     /locus_tag="QGF01_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20215.1"
                     /translation="MKKEYVVVRIDAAPDAAPYVVVSLSSKKDMQESDGPLSSPFGKP
                     NVMGFTNMNDMAKELNKMMAGGMPGMMGGNTTSLKLDMMEYKDLGLSVGDKVYLELSK
                     AENSGV"
     gene            10753..10827
                     /locus_tag="QGF01_04680"
     tRNA            10753..10827
                     /locus_tag="QGF01_04680"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10788..10790,aa:Pro,seq:ggg)
     gene            complement(10828..11586)
                     /locus_tag="QGF01_04685"
     CDS             complement(10828..11586)
                     /locus_tag="QGF01_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HJN20216.1"
                     /translation="MKQSFLKINEITEPPHSNIWVYPRGTKAQIKSRIKELESLGVES
                     VSFQGELKIDTINVLGKGYVGIVVLCKIGKKEVAVKIRRIDSQRKNLKKEAEFLTITN
                     QINVGPKLLGFSKNFLVIEYLDGEKIGKWINKLKEKPNVAQLKKIIKKTLTDCYKLDK
                     IGLDHGELSNITKHVIVGKKITILDFESSSVDRRVSNVTSATQAFYIGSGISKIVNPL
                     CKPSRKSKIISVLRKYKTDQTKENFLDLLKVLNL"
     gene            complement(11567..12895)
                     /gene="cca"
                     /locus_tag="QGF01_04690"
     CDS             complement(11567..12895)
                     /gene="cca"
                     /locus_tag="QGF01_04690"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HJN20217.1"
                     /translation="MNPILNYAKKITVPSQRLQEEKQQIAKKVCDLINESIGKYNQIV
                     GFALGGSYAKDTWLPEKADIDVFVKFNKKTSEKDFRSIGTKIGFESLKKYKPYTRYAE
                     HPFVEAVVNGTKVNVVPCYDVNVGEWQSATDRSIHHTKFMSKKLTNSMKDEVRILKKF
                     FLHIDVYGAELAKEGFSGYVSEVLISYFGSFEKTLKKISKLKQGDVIGKSAKKFSSSI
                     VIIDPIDSNRNLGAAISVESLGKFVLASREFLKKPSKKFFNEPTPKRNMKNIDKIVVV
                     QFKFKQKSDDIIWGQIKRASNALKTQLELDGFTVLRNSSAKDEKENGALIFLLQTKKI
                     EESFVRVGPNISSEDHCEKFISANLKRSELMWINEENKIQSLQKRKYSDVILFLNNLL
                     KTNLKNSGVPKGLEKDFKKGVKVVPANKVSNKSINEAIRNIAVTDETIFS"
     gene            complement(12892..13431)
                     /gene="thpR"
                     /locus_tag="QGF01_04695"
     CDS             complement(12892..13431)
                     /gene="thpR"
                     /locus_tag="QGF01_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HJN20218.1"
                     /translation="MRIFVAIEINDKNVLDSISKIQSELKINAKPVKIQNLHFTVQFL
                     GEVSDQMVEKISRDLCDLEFTPFSISFSGVGVFPNPNNPRVVWVGINDGVSELEELVV
                     DIQKKLSCFGFSPDKKFKPHVTIFRIKNKIGDITNELEKLSGYYFGKQMISEVKLKKS
                     ELTPNGPIYTDLLVVKGRK"
     gene            13505..14056
                     /locus_tag="QGF01_04700"
     CDS             13505..14056
                     /locus_tag="QGF01_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN20219.1"
                     /translation="MVKLIVCLTGMPGSGKSTIVSVLKSKGIETLNLGDGVRAEAKRR
                     NLEPSGENLGKIMLELREKNGPGAIANLLTDQIQNSQSNIIIIDGIRSIAEIEVLKNV
                     GSVKLLSIEATADTRFKFLKARGRSDDPETKEKFAERDNREISVGIDKSISIADETIS
                     NNDLTLDELTELAHTVIKKWIDN"
     gene            14053..14454
                     /locus_tag="QGF01_04705"
     CDS             14053..14454
                     /locus_tag="QGF01_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HJN20220.1"
                     /translation="MTPLNVDCKISAYCMINISEDVNKIQTAISNVLTDMDGRVSGNS
                     FISNSNNYESLTKIYETIRGNNSQNTYRRHLRANIDKNSTWFYLNKQAAFVDVIAICN
                     EADESPLGPIKIVLHSENIEDVIEWLVSDTE"
     gene            complement(14551..15450)
                     /gene="rnz"
                     /locus_tag="QGF01_04710"
     CDS             complement(14551..15450)
                     /gene="rnz"
                     /locus_tag="QGF01_04710"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HJN20221.1"
                     /translation="MEIIFLGTGGAQPTLERNTTCMCLIREGEIMMFDAGENAQISFL
                     KSGLGWNKKMKIFVTHLHGDHCIGILGFLQTMTLQNRTEPIEIYGPDGIEEFIATNMK
                     VMNFGLSFPVLITSVNNGLVVNEEKYEVYACKAEHSVTAFSYLFKEKEKPGEFYPDKA
                     IALGVPKGKLWHTLQCGEEVTIENKTIKPSQVMGPNISGKKIGVSGDTRPTKELEEFF
                     NACDYLIFDSTFSDELKEKAVESGHSTAKEAATLAQNAKVLNLILTHFSARYNDDKVL
                     LEEAKSIHSSVIAANDQLKIKIS"
     gene            complement(15453..16328)
                     /locus_tag="QGF01_04715"
     CDS             complement(15453..16328)
                     /locus_tag="QGF01_04715"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HJN20222.1"
                     /translation="MVNFTVIGGNASKGLAKRIARRLKAKYVDADTRTFPDGESKITF
                     RHNLKKSVVLVVQSTYPPVDTNLLQILSIISEAKKISSKIYAIMPYMGYARQDRQFLS
                     GEIVTMSVVAKMLQAAGAKKIIAVDIHSKTALRHFKIPTENVSAIPELAKYLKKLELK
                     NPIVVSPDTGGSLRAKKFADILKSDSITLKKSRNRKTGKVSIRSTKADVNGKDLIIVD
                     DIISTGGSVVKATQFLKKQKCKRIFVVCTHGLLIGDAEKNIKKAGVTQIISTNTIPRS
                     ISKVDVSGVIAQAVQ"
     gene            complement(16370..18091)
                     /locus_tag="QGF01_04720"
     CDS             complement(16370..18091)
                     /locus_tag="QGF01_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HJN20223.1"
                     /translation="MVDDLSEFSAGEFQTVSDLLASDTSLQILMGVLVIGLVILASSY
                     RGFGKWMSSKEFSYTRPHVARFARTAMLSVFAIGLVTSINLFIQFTETDAVNPSSVEA
                     LETFAKILNTINILVIGYTVSHLIPIGLNKAEKTKLEAEDFEKWKDMKGFKDDEDGLF
                     HKIFKWIPPKTPPEDLTMEDFEKNLQTKEGLNFLENYRTSKGVTIGSYEKTVKDPIED
                     WKEAERKKYQKYLNDCRSGNNETGRKLVLGTKPQEIYAVDVWREVRRQKGYDPIIPAS
                     KPAGHQQLQEERVPKSAKQVLPIGIFVATVIGVVGWWGVDLFVLATATGGMALGVGLA
                     LKETLENYFAYILIRKDKVFTEGDRVQLESGYNGYVHRITPRVTYIRHGLNESLAIIP
                     TRQLVTAEIINFTKEIQLVPAVVDVGVSYLNDPKQVASILVKIGKRALIEVKNSKGSH
                     VAAQKRCPYLDQNKPSCGCDKDIHVDVDQPTVRFNKFNDSSLDFSLWVYVNSYGAQFQ
                     MKSAMRLIMYEEFKKYDIRIPWPIRTVYQGDEKRESQEISEFEDQRKQAVDEFGMGDL
                     ARGEGDE"
     gene            18245..19564
                     /locus_tag="QGF01_04725"
     CDS             18245..19564
                     /locus_tag="QGF01_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HJN20224.1"
                     /translation="MTEQQTTWSINETTPIKSYTLSNFRTIPQIQQMSEEAQFEMEVV
                     GNVLPFKTNNYVVEQLIDWNNIPNDPMFVLTFPQKGMLIPEHYDKMASALKSGADRKE
                     IQKVANDIRLKLNPHPAGQMELNIPQLKDGTKLYGMQHKYDETCLFFPSQSQTCHAYC
                     SFCFRWPQFVGMDEMKFAMKEGEQLVQYLREHPEITDVLFTGGDPMIMKASMFSVYTD
                     ALLDAKLPNLKTIRIGTKAVSYWPYKFLTDSDADETLKNFEKIVKSGIHLAIMAHFNH
                     LVELSTDPIKEAIKRIRNTGAQIRTQSPLLAHINDDADMWAKMWEKQVSLGCIPYYMF
                     VVRDTGAQHYFEVPLVKAEEIFRTAFRKVSGLARTVRGPSMSATPGKVHVLGISEAAG
                     QKVMIFQLLQGRNPKWVGIPFFAKYDENAIWLDDLEPAFSEKFFFEE"
     gene            complement(19592..21139)
                     /locus_tag="QGF01_04730"
     CDS             complement(19592..21139)
                     /locus_tag="QGF01_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HJN20225.1"
                     /translation="MRYKEFLIFVLPILTLTIFFVIPDSYGHGLGYEVLPPVDLGSKQ
                     VALEISSNPIPDSDAREISFTLFDTSNGVTINDVTFFVMAIKQNQTLFDVTGQRDDGI
                     FVIRLIPTESEQIITNEEDAGLFGSILGPNKIVNVYGNTFDSGGLYNFKILITTAEGY
                     TNFLSPPIDYDAGISFPDTTYYDIDDPNFGQQKLGIVTYYEQIKNDFNYNEQNRIISY
                     SMPFDWSEENMKQVSVIHQELLIPKTFGDLMVKGFSAKVNGLDILDSDITVDDFSDEN
                     RIMHLVISQKEILELSQKLQNSNKIKFDITPKDENLLSTITENGQFKINLSWEPKKIE
                     SGIKTTFIFDILDVFLLDKPVSVNYDLSIIYDGKKLLQKNGISTDLRTEHNTIEFLVP
                     ENTSGPIILQFENLDENELANASLPVIVNRIDTGEISIPAWIKNNAGWWANEEIPDSA
                     FLQGIEFLIKEGIMVIPPTASEAIESQEVPAWIKNNAGWWANGAIDDNTFVSGIQYLI
                     KNGIIVV"
     gene            21249..22160
                     /locus_tag="QGF01_04735"
     CDS             21249..22160
                     /locus_tag="QGF01_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HJN20226.1"
                     /translation="MVNKNKTKEKCPRCAKGTLVTDVESGEMFCSKCGFVLTDKVQES
                     GPEWRSFTQDEHGDRARAGAPTSLTMHDMGLATIINPANKDAAGRPLTSTMKSTIERL
                     RTWDSRSQVHEPVDRNFRQAFSELNRLKDKLAISDAVIEKAAYIYRKALDKGLVRGRS
                     ISALMASALYAACRDTETPRNLKDVEKVANIKRKDIARCYRLLVKELDLKMPVTDSVQ
                     CVARIASRIGIQEKTKRYATKVLQAAQKNEVSAGKDPMGLAAAALYLACVKNGEDKTQ
                     RDIAESANVTEVTIRNRYKGLKEAFTM"
     gene            complement(22161..23618)
                     /locus_tag="QGF01_04740"
     CDS             complement(22161..23618)
                     /locus_tag="QGF01_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJN20227.1"
                     /translation="MNLVFFALLDDLVSFFKDGVFSNIDFNDFSGSNVRDMLKSYFEK
                     NPIPEASTEQFDSNFLLQGIVNLQNIIADVSFGDSLVASAPILLLAASVVIIMGVLGE
                     AFFKRTGIPDILFLMVLGIIIGPILGIIQPEAVLQIVPYFAAVALIVIMFDGGLNLHI
                     GKVLKTAHFAVILVIVGFAVSVGIVAGLAHYGLGWEWIDSILLGSILGGSSSIIVFGL
                     VKKIHISEDAKSMLGFESALTDIFAVIIAFVLFEAVLSGEFSLDLLGITIGKAVAVGL
                     VLGLGVGIPWMFVISKLKNAQHSYMLTIGMVFMLFFLATSFGESGALTALVFGLMLGK
                     KTYFSRILKIKFPEDTIDNSLHSQVTFLVRAFFFVFVGLLASFTQIEYVIFGVIAGIA
                     IYIGRIILTKTVLVRGFSKLDRKVTSVMIPRGLAAAVLATFPLSMGLPNAEAYPQIIF
                     FVVITSVIITTIGLGGAKKIPPPESQDGGYIQSEK"
     gene            23742..24284
                     /locus_tag="QGF01_04745"
     CDS             23742..24284
                     /locus_tag="QGF01_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HJN20228.1"
                     /translation="MSATDTLRDDHKQIKRLDKIVSKCSSDIYAGKTIPFHDLEKITL
                     IISEFLDSIHYSREEDSYFPCVASYDHLKKEIRALLIEHEFSRNIAYKITLHLKRWKN
                     GEDSSEPVARFLRTYSIYLNDHISKEEDFFERAEKEIISKEEEKDMFEQFQSDMAIAK
                     KMESILEDIDYLENQMWFKN"
     gene            complement(24287..24496)
                     /locus_tag="QGF01_04750"
     CDS             complement(24287..24496)
                     /locus_tag="QGF01_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20229.1"
                     /translation="MDKQKKNKRNVIILLASLWLISTLPLPWLFTSPDITQDQIIGLM
                     PIIGIISIPFIALGIAWTLKPELTT"
     gene            complement(24533..24988)
                     /locus_tag="QGF01_04755"
     CDS             complement(24533..24988)
                     /locus_tag="QGF01_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20230.1"
                     /translation="MSKKPIIGGIILVVIIGVIFVGAQINPDNSDNIQFGNSDIFHVT
                     LADPALYENGVYVEVFDVKEGTYQFGFVPNGDSPKKLSIKLMGKTISFYEDFELQGTL
                     VQDEMSSWYTWEYLGEKNVSSGESQKVKIIIDPNGSLKGPVSIQLIPMK"
     gene            25091..25738
                     /locus_tag="QGF01_04760"
     CDS             25091..25738
                     /locus_tag="QGF01_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Snf7 family protein"
                     /protein_id="HJN20231.1"
                     /translation="MTSLSSKWSKPQKAGISERINNIIKPSGALKPRVETAIKKLQAQ
                     VAKLDVMLSSLNSRDEKIFHRIVTATQQHDTHTSKALSKELAEVRKVRRVLGNARMAI
                     EQIELRLTTFHDLGDTVTTVMPTIGLMKNMKTSLAKFMPGADQELNNMAEMLGGLMTE
                     TFHSSDASFGVDTAMNSESEKILQEAGAVAEQQAGDKFPSMPEEISERSSSSRFM"
     gene            complement(25754..26320)
                     /locus_tag="QGF01_04765"
     CDS             complement(25754..26320)
                     /locus_tag="QGF01_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJN20232.1"
                     /translation="MGFLDIFKDKDEDENRDDFMRKFAGMEENSNFDSERGPEPAQEP
                     SSYSQPAEEPSSYSQPAEEPSSSSQIGIGSLMDKRAKLEDAIDYVGLMIKNLKDKRTR
                     LEKDIEDESVDIKNLKEKLSKINEFIQDENNGLEELTNKRAGVEKEADEVGNIIYGQR
                     DKISGIDKILADEADKIKRFKESKSNSG"
     gene            26512..26799
                     /locus_tag="QGF01_04770"
     CDS             26512..26799
                     /locus_tag="QGF01_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJN20233.1"
                     /translation="MSKQQYRSEMGIMGDILDVTMDGGQQGVIVSAISRRANLSHYSV
                     LDKCEKLITAGLMESRRNERNRLFKITEKGLGFIKEFQRFQTIVDSMNLRY"
     gene            26803..27828
                     /locus_tag="QGF01_04775"
     CDS             26803..27828
                     /locus_tag="QGF01_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HJN20234.1"
                     /translation="MNLQQIVAQKHQSPGGEMLLVGNTDILSAMVRIPIVVIGLVVVA
                     LSIPIQQSLGSSRTLDFTIYSDGSTHVFYELETNPLEPEFSTKLFGTTLENLTIIDNN
                     GFLLSYETVGDSVLIETLGASHIFIDYDTQELVSKTGKIWKFSVDPSSEYSLLMPNDS
                     VIVEMNNFPISMEVENEQSRLVFTPGQTNISYYISTLASAQTTESISEDGGTDNSSIL
                     WILVGVISSIVIAAAIIVIKRKSGTPKSSVTIQKDEEPKLQSLDPESIFKAKQDLRED
                     DKEIINFISANGGQVFESELRKKFLQPRTTMWRAVKRLERHGIIEIVKKDFQNVIKLR
                     STLEDDS"
     gene            27825..28856
                     /locus_tag="QGF01_04780"
     CDS             27825..28856
                     /locus_tag="QGF01_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20235.1"
                     /translation="MKKLFFFFLLFLIASMTIVTIPHNVSAESQLDILVRITEHTKQY
                     IKNDMNKIGNISNEIQQLYDKGLKEIDLLTDAAENEDAVSAKQHFHAAMTTFKKIRIA
                     ISGLESETVPLQHSAIQSQTIKKYEWNIKKLKDVNLKLNANINFDSIDGLMNLAKKNL
                     TEGDIGQNQQTLEKIADKGREIQKLLYDISQEGKFDRAKQFAIEYTKNVDQLIESAKK
                     MGLSDTVGKLENSKTIIIHANTTNQIVNQHKIIIILKYKIEQTNYLQQAKLLQLKSVI
                     SSLENQAKILADQVTEDNAAKYFLKNIFYLIDEAKKDLKNSPESVPGKITEIKNLLIK
                     TEKLINNSS"
     gene            28888..29082
                     /locus_tag="QGF01_04785"
     CDS             28888..29082
                     /locus_tag="QGF01_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20236.1"
                     /translation="MASTKIIVCVGIPMIVTGFLIAILWAPTAYGVESTVEFVGSLIG
                     IIGVIFVIAGLFYTKEPVAA"
     gene            29140..29853
                     /locus_tag="QGF01_04790"
     CDS             29140..29853
                     /locus_tag="QGF01_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HJN20237.1"
                     /translation="MKTRLFEIFTSIEGEGILYGTKTLFIRFAGCPFSCFYCDTPDAL
                     PSDSGKEYSISEACDLIDKNLQNNTYKVNFTGGEPLVQHESVNELAKHIKSKGILTYL
                     ESSCFDSQKFSYVLPSIDFVKIEFKTKDSEMVDTKHYTDLMKNTLECLHTSIKMKKIT
                     YIKIVVSSKTELDNLKTLLKQIFKIASRETISGFIIQPTYGVSEPSLEKLLEFYDLVY
                     PYYRDVRVIPQLQKIIDVP"
     gene            29933..30490
                     /gene="folE"
                     /locus_tag="QGF01_04795"
     CDS             29933..30490
                     /gene="folE"
                     /locus_tag="QGF01_04795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HJN20238.1"
                     /translation="MDEEKVKKLIRELIIEIGEDPTREGLAKTPERIVGVYKEIFNGY
                     EADSELSVQFSEDSEIVIVRDIQFYSMCEHHLLPFFGKIQIVYSPDGRVFGISKLVRL
                     VEKFSRRLQIQERLTKNIADELYAEGVKGVAVLTEAEHLCMKMRGVRNNANVTSGAFR
                     GIYERKEEKERIIELIKNPTKSSFQ"
     gene            30533..30859
                     /locus_tag="QGF01_04800"
     CDS             30533..30859
                     /locus_tag="QGF01_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20239.1"
                     /translation="MVGRDNAHIPKESWPAFTWYLIEFAIVFAVANLIAFQIIPMVDG
                     MVGEKSLLICEQESWKSNCAPSEEKMSDTGLLNWIYWGMVGGVFIGWYLLIRGFILKK
                     PILGNR"
     gene            complement(30856..31533)
                     /locus_tag="QGF01_04805"
     CDS             complement(30856..31533)
                     /locus_tag="QGF01_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HJN20240.1"
                     /translation="MKKAVVVFSGGIDSVSMCAYLKSKYEMYGISFLYGQKANQEIKK
                     AKRFAKILGLKKHKIVNIDFMKDLYGDTNVLTSSKKNIPGKFDYSIVVPIRNGIFLSI
                     ATAWAFSINASLVAYGAHMGDKNYPDCRPVFSKKIESSFNQGEIDGIKSGIRKKIEIW
                     TPYKAKLSKKLLLKKGYNILGDNIFNTWSCYSNEKVQCGKCESCNNRKAAFLEANIED
                     KTVYRYD"
     gene            complement(31535..32479)
                     /locus_tag="QGF01_04810"
     CDS             complement(31535..32479)
                     /locus_tag="QGF01_04810"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HJN20241.1"
                     /translation="MVTSVKVRAPSSTANLGPGFDVFGLALDAFHDEITLSKTNKSTT
                     NKPWHGIRILTTDDVPKDPQQNTAGLVIKSMKQKFKIKSGIEMKIKKGVPAGFGMGSS
                     AASAAAAALAFNKLFNLKLDNKTLVKYAGIGEKASAGTIHYDNVAASLLGGFVVVKTK
                     PFGVIRLEPPKDLVLCVAIPKLKVPKKKTQVSRAVIPKTVKLSDLTENLSNAANIVSG
                     FLLKDSDLIGRSIQDVIVEPARKHLIPGFSKVKNNALRAGALGVTISGAGPSIIAFCK
                     KSQNVKKVAKSMEKGFSSAKIDCDTIICKPSMGPKIRV"
     gene            complement(32479..33237)
                     /locus_tag="QGF01_04815"
     CDS             complement(32479..33237)
                     /locus_tag="QGF01_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HJN20242.1"
                     /translation="MKAIFITGTAGAGKSILASRILQYYEQNGVFASILNLDPGVESL
                     PYKPDVDVRDYVDILAIMKQYDLGPNGSMIMASDLIASKIEQIENQVNKVNPDYLIID
                     TPGQIELFAYRTSGPFFVHNINADEKLNLFLHDGTMVTSPSNFISIALLAASIKLRLG
                     LPTVNLLTKSDLIADKLDQILNWSSNPMQLEESIGKDLDGEMYSLSMDIIRSLNLGNF
                     VDELIPISNVTEEGMINLESAISRIINLGEEVED"
     gene            33296..34393
                     /locus_tag="QGF01_04820"
     CDS             33296..34393
                     /locus_tag="QGF01_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HJN20243.1"
                     /translation="MNWRIAVIPVTMIPIIIIIIQFDIKLDDVFAIGILPFIAAILAM
                     MVKLGLQGLKLSYIARSFLGNFDSIKRLAAMRIGSEFIKFTTPMFVGAEFVVIYYMTK
                     KGISPSKASWVALLDIVTEVFAGGLLSILAGIFALLNGAYVVAAIILGTSIFVTTLWV
                     VLFFASAKHTFHLPKGIAMLLTKFGGVRGEKYAKQTDEWIGEVCTMSRENLGSKKSKK
                     VFWVGFFMSLVSWTFYGISFLAIAIGVGMTIELFESVMAVMGANAIGNLPITIGGSGL
                     AEFGIVAYLNNLNPFNLVIPEEGLEWNAVIGWRIATYYIPIAVTWILLMKLALGKFVK
                     PKTLHQKCPICGNMINDIEFASHMQQKHSTA"
     gene            34428..35234
                     /locus_tag="QGF01_04825"
     CDS             34428..35234
                     /locus_tag="QGF01_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJN20244.1"
                     /translation="MNFKNKVIVITGASSGIGEASAIKFAKKNAKIVLVARRKEKLLQ
                     LQKKLVKFTDSTLVCQCDVSNKTQVKEMTDIVLDTFGRIDVLINNAGFVIYGKVSDLS
                     IEEIESQMETNYFGMIYCTKNFLPHMIEQGKGHIVNVASVGASFGVPGVASYCATKFA
                     MLGFSEGLKHELSGTGVDLTVVSPIMVDTPLFDHPSFENFSKRSTLVTLSPNTVANAI
                     LKAANSSRLEIVVPSVARVGIWAKQNFPYIINPIIGSSFRKQLEKRNKSK"
     gene            complement(35253..35624)
                     /locus_tag="QGF01_04830"
     CDS             complement(35253..35624)
                     /locus_tag="QGF01_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20245.1"
                     /translation="MAKKKDDIPGWVTDEIKNAKFGKPEQLTRNGYVLEVYDKDNKID
                     AQFYDPVEDGRSIVTLDLSKKIKMDKLEKGVVYEFKFDSLKAPLDKKVIEYLKTEKEL
                     EMDAIYQFELTEITIVDDGSR"
     gene            complement(35637..35951)
                     /locus_tag="QGF01_04835"
     CDS             complement(35637..35951)
                     /locus_tag="QGF01_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20246.1"
                     /translation="MPNCPECTFRERKKLKMQFEAEAEKAREEDAEYVMPHDRDIGSD
                     LEYPMKLDPSTKHFICKRCGLYATREQIFDIRDKLNQRESTKEDKQYDYLEWWQKSKK
                     DK"
     gene            complement(36108..37343)
                     /locus_tag="QGF01_04840"
     CDS             complement(36108..37343)
                     /locus_tag="QGF01_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJN20247.1"
                     /translation="MKKIGQFIYPWGNGHYSRMMRLNAVLPNYLTEKPEMFYFSKGEI
                     YEKLLQKFPDKKDNIHEILMPTPIDGKNGPSVPLSLLNILFPVAKEKALVKQIKHYLK
                     KEAQIYDKEKFDVVINDGDMGSNVLAKNRGIPSLFVTNQYMPKLWKSRSYFYPALYFV
                     AKQIEKASMILVADSPPPYTICEYNLNFPESIKNKVTYVGHFSGKASVSSEPSTNLEN
                     MVKETDFGYWMRTGNKSTNDGTGQKYEEVFHDSEMKNERRIISHAKNDKSIDQVIGKD
                     GKKYSLLDAYEKKIDWIQIDIGFLTENEKQTVINNCKFCVINGSHTVMGEIMGTNSKP
                     IIGIPVYDEHTNQIQWAEEKKLGLLAKDKKQVIKAVKKIKHDYNKYEERLKEFSENFD
                     NNGAEKTSKIIAEILEKKN"
     gene            complement(37349..38491)
                     /locus_tag="QGF01_04845"
     CDS             complement(37349..38491)
                     /locus_tag="QGF01_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein"
                     /protein_id="HJN20248.1"
                     /translation="MISILENNIVVVVFPSIFSQNKIGPLITNIKHILKIQNQKFQKI
                     RQDDKVIIVETDDPVFVSSTINDLFGIKNIAIAKQVKNNFKIIVENITKIATDLFLKN
                     ERFFLQVEGYSKGFVTRDVEVAATSSLIEKTVKKGIKPGTAEKYDRRLFAHLTKSSAY
                     ICIYYDKGLEGIPNNSQNKKMVCCVFDELSAISCIETIKQGFDVKIIVCYNKKSELLD
                     LVKMINVIIHKTVQTQVSLEFYKINAGVKLPPMVLAEVIAGILVKVAISVNIKRISLS
                     ISPIIHPLSFFEKIVKQIHSKGLISYTPLTTLDEDIIDDAKEIGLEKYLNKIEKIGKT
                     KFISSASPKKKIENLVERSMKTKKIVHVKLGVNNIHDILDSVSAEE"
     gene            38567..39286
                     /locus_tag="QGF01_04850"
     CDS             38567..39286
                     /locus_tag="QGF01_04850"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HJN20249.1"
                     /translation="MAQFTAEQLDELNDKLKTPEEVLTWGLENIHPKLALASSFGAED
                     VCVIHMLAKINPEARVFSLDTGRINQETYDVMDEIRKKYNTKIEITFPDATEVIEMVQ
                     THGMNLFYESAENRKLCCGVRKVHPLNKMLSTLDGWITGLRSDQTQNRQSSTKIEIDE
                     QHDGMIKLNPILDWSWDQTMKYIKDNDIPYNKLIDQGFPSIGCEPCTRPIKPGEDLRA
                     GRWWWENQSDKECGLHMDHNK"
     gene            39313..40440
                     /gene="sat"
                     /locus_tag="QGF01_04855"
     CDS             39313..40440
                     /gene="sat"
                     /locus_tag="QGF01_04855"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HJN20250.1"
                     /translation="MISAHGGKLVNKVTNTDSSGLFSIDISADLANDVENIADGIFSP
                     LEGFLNQQNFESVISKGRLVNNTAWTIPIVLDADDETSKKIKEAGDVLLKNPEGTGIA
                     ILHVEDIYAFDKKNTANGVYGTNDESHPGVAKTYGMKDFLVGGEIDYIQKPNASEIRK
                     YRMTPVETREAFAKAGWGKIVAFQTRNPPHVAHEILQKTSITTRDGVFVNPLIGKKKS
                     GDFKDEVIIKAYEAMIENYYPENRCRLATLHTEMKYAGPKEAIHHAIMRQNYGCTHII
                     IGRDHAGVGNFYDPFAAQKIFEEYPELDISPIFFPAFFYCRKCLTFTNPKVCPHDAES
                     REQISGTKLRSMIQEGKAPSEFILRPEVAKVIIDFDQPFVD"
     gene            40472..41164
                     /locus_tag="QGF01_04860"
     CDS             40472..41164
                     /locus_tag="QGF01_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20251.1"
                     /translation="MKYILFLLVLLICTTSPVFAQEPKIGDFADPVNPSLFTEKIEIP
                     YHVFNTISRDAEITELENQITGSWQLSLQNDLLYGNPNGNAVIRIYDVDIADKFLEIG
                     MGSYPDYKYWISAQVPETGYVIIYSNNDNGWIKGTETSITYSEQNGLSVNNGQRTVLS
                     NLDVSPFAIKAYSAYGMESSSDPPATTSGTFIIKIISADYGENPYSIFPFVLTGIIGL
                     VIGILVLTKKRS"
     gene            complement(41148..41801)
                     /gene="nth"
                     /locus_tag="QGF01_04865"
     CDS             complement(41148..41801)
                     /gene="nth"
                     /locus_tag="QGF01_04865"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HJN20252.1"
                     /translation="MKKILDGMKKTMVDVNPPRYTALEGLHKVQTARPFRILIATILS
                     ARTKDENTTKAADKLFKLYGTPQKLAKAKVRDVEKVIKSVGFYHVKSKRIIEVANLLL
                     SKYNGKVPADIDKLVDMPGVGRKTANCVLVYAFEKPAIPVDTHVHRISNRLGLVDTKM
                     PEETEMALREKIPKKYWLDINNTFVMYGQNICKPISPMCDVCKIRKTCNYFQTKSVS"
     gene            complement(41821..42390)
                     /locus_tag="QGF01_04870"
     CDS             complement(41821..42390)
                     /locus_tag="QGF01_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20253.1"
                     /translation="MKRRHLHESDRQYKIFLYIFYLCMIIMGSITFYGIYAMSEEWNE
                     NGIYVMGEVLVQTQVPFENFANLSTWLFFSTIIGWYCCSRIGWKKTVGLNSYRMALTQ
                     LMLLGFAIICLYEVLWSFTVLNAEITSQMILQNTIPDIDLLMVEYPDPLRPWNLIFAT
                     KIWLAGFVISAHAFYLSTRQRKTLEDLDE"
     gene            42470..43555
                     /locus_tag="QGF01_04875"
     CDS             42470..43555
                     /locus_tag="QGF01_04875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HJN20254.1"
                     /translation="MNVPINIPDIDEQEIREVTSVLSEKALTSASFEGGKRVRQFEDL
                     LSNFVKSKFAIAVNSGTSALQASLYALDIKPNDEVLIPSFTFVATANSVKSIGAKPVF
                     VDILKDNFTMDPDDIKKKITKRTKAIIPVHLYGHVAYMEEIMEIAKKNHLEIIEDASQ
                     SLGSKFKGKHSGTFSHLGCFSMYAAKVMTAGEGGAIVTDDKKLFEKLKQIRNHGLSKH
                     HITSKFGLNLRLPEINAAIAKIQMKKLPTFLRQRKKNAQILTELLQNHDIILPKERKN
                     EVMNWYLYTVALKQRDKIMNKLNSAGIGAAVYYNPSIHQTPYYKTKKTLTTTEWASHH
                     VISLPIHPKVKKSDLLKIKKILSAYRN"
     gene            complement(43557..43889)
                     /locus_tag="QGF01_04880"
     CDS             complement(43557..43889)
                     /locus_tag="QGF01_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HJN20255.1"
                     /translation="MADKLDEIFTLQKSLERMMDLDRYPKDTEAKLSALCTAMMHEVV
                     ELQRTTNWKWWKKPTSFNQSEAREELIDIWHFLIQASMELGLTPDDILKEYQRKNEIN
                     KQRQKDGY"
     gene            complement(43922..44815)
                     /locus_tag="QGF01_04885"
     CDS             complement(43922..44815)
                     /locus_tag="QGF01_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HJN20256.1"
                     /translation="MLTVFGSTALDTIRTPTKILKDALGGAATFAGISSSFFTKTGLI
                     AVIGKDFPRKYHRILEQYFDLKGLSIKNGKTFRYDGRYNKTFSTRTTLRTDLNVLKNF
                     KPVVPEEYKKSKFVYLANNDPEQNISLIKEFDDVKFSMCDTIDFWINTKRASVVRMFK
                     EVDAVVINDEEAKLLTKEFNLIKCAKKIMGWGTKYVIIKKAEHGSLLFYEDAIFPSPG
                     FPLGNIVDPTGAGDSFAGAMIGYLASKKKTNLSTIKKSIMYGNVMGSFAVQKYGLGRL
                     LELTKADITKRVKQYEKMVSF"
     gene            44944..45414
                     /locus_tag="QGF01_04890"
     CDS             44944..45414
                     /locus_tag="QGF01_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20257.1"
                     /translation="MAKIKIKIGENEIEVDSRDFYIDNNTIGEVIENISIYLQGSNKN
                     FFEQIPIQENGEGMNKLSLLEDAEVHEPEFSDPVLVTIDELHGKLVTLSTNSFFDQPR
                     TVSETVSELRENGWIASPLDVSKVLAKMAFCKELLKNKQDDRSFYFVKEALLAE"
     gene            complement(45579..46064)
                     /locus_tag="QGF01_04895"
     CDS             complement(45579..46064)
                     /locus_tag="QGF01_04895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJN20258.1"
                     /translation="MAYATIETNFGNIKFKLLPELAPETVRNFRKLSDSQFYDGTLFH
                     RVIPGFMIQGGDPNTKQSNQSNWGMGGPGYAINAEFNPRSHLTGIVSMARATDPDSAG
                     SQFFIVTSDNNTASLDNQYTVFGEVTEGIDVAHKIENLPRNNNDCPLEEAKMLHVSIS
                     E"
     gene            46185..46403
                     /locus_tag="QGF01_04900"
     CDS             46185..46403
                     /locus_tag="QGF01_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20259.1"
                     /translation="MNRTNQSTIIKAEIDDLLTSGVKDKQEIYSKVVDKLGVPRPTVR
                     RVAGDLRNELLAKISILQSETQTTEKTE"
     gene            46489..46746
                     /locus_tag="QGF01_04905"
     CDS             46489..46746
                     /locus_tag="QGF01_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20260.1"
                     /translation="MGYLYNHLATVVCAVFAGVLTLLWPLFVDFAAVLDLVFVMAVPI
                     MWFLTAVCFVAQKSTDYVHGHQSSHKSTKKLIYTSGGQVSN"
     gene            complement(46931..47022)
                     /locus_tag="QGF01_04910"
     tRNA            complement(join(46931..46990,47008..47022))
                     /locus_tag="QGF01_04910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46968..46970),aa:Arg,seq:cct)
     gene            47157..47230
                     /locus_tag="QGF01_04915"
     tRNA            47157..47230
                     /locus_tag="QGF01_04915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47191..47193,aa:Val,seq:gac)
     gene            complement(47258..47752)
                     /locus_tag="QGF01_04920"
     CDS             complement(47258..47752)
                     /locus_tag="QGF01_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20261.1"
                     /translation="MKPPGKIILYIAIVLLLAFMGGMVYVSLLDIPELEKSELKLGSI
                     ELVNVDSIDNKIKLKVVFLLKNHAEKTITVPVVSYELFINGKSMGSSSYSSEDIPMTG
                     RIPFFPGKELPLDSYIVIPLSDGNQDEYSAIVNGEDVTYSVIGQYTVETAWQLIEVNF
                     EKSL"
     gene            47834..48328
                     /locus_tag="QGF01_04925"
     CDS             47834..48328
                     /locus_tag="QGF01_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HJN20262.1"
                     /translation="MAIEQTIITWIHLLSASIWVGGSIFLGAVLAPLLKKMSLSIDER
                     LALMIKTGRRFNKIALPSLMILIATGIYNSHLVLQSPEILFSSSYGAYLITKIILVIA
                     LIITFAVHIRIFSKDVEEKIIAKQIPDNELQKLNKKGMILGETTVIISVTILFFAALL
                     NTGI"
     gene            48362..48856
                     /locus_tag="QGF01_04930"
     CDS             48362..48856
                     /locus_tag="QGF01_04930"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HJN20263.1"
                     /translation="MNGLLIGRFQPFHLGHLEAVNFALDKVENLWIGIGSSNKYNEKK
                     NPFSADERRKMITLSVNDSIITRIKIFDIPDVDDHEKWTYSIDKIVPEYNLVFSNDKF
                     TDNLYKRRNIDVITVPLKDRQKFSGTNIRELIADDKNWQDLVPQGTRKVLDNLNAKER
                     LKDL"
     gene            48898..>49607
                     /locus_tag="QGF01_04935"
     CDS             48898..>49607
                     /locus_tag="QGF01_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJN20264.1"
                     /translation="MEYLSMLPGPTNVPNRVMRAMLAPIINHRSDDFVELYTDVISKT
                     QQVFETQNDVVALSASGTGAVEAGVVNIVKKDDKVIIPVNGEFSNRLSQLIEGQGANV
                     IRLETPPGENATFDQVKEAFDNNNDVKAFYVVHNETSTGTMVNYLDKVSDLTSRNDSM
                     YIVDSVSLLGGTSLPVDKWNIDVCMTGAQKAIAAPPGISPISISPKAKKYMIENPPAT
                     MYFNLARYFKYYEESKHTP"
BASE COUNT        16554 a         8782 c         8162 g        16109 t
ORIGIN      
        1 gatgcaggta tgggtgatgc aggaaatgat ggttctggtg acggtggtga aggcggattc
       61 atggatggtg ggttcgatgt tgggttttag ccaaaaaaca gttaatttta ctacttttta
      121 gctctttttc tgtctaaaaa tgcctatttt tgtatgtttt ttgacttgtt tttaggcgta
      181 attatcgaag ttttacgtat tcgtaagctc caagtttgtt gtcaaaacaa tattgtgttt
      241 tttggaaatt ctttctcaac tctaaaacat cactcgcaac ttcctttgca tctttgttgt
      301 caataaccac gtttttgata agcgaggcaa tttgctgcat atctgattct ttcattccga
      361 gccgagtaac ttccgaagat ccgagcctaa ttcctccagg attcatgtaa tgccttttgg
      421 ccttgagatc acctgggaca agctgcctat tcacaatgat attcgccttt tccaagtcag
      481 cctcaatctt gccaccatca ccataatcaa gcacatttac aacagcctga tgggattgtg
      541 taaatccctt cttttcaccc aaaaccttga accctaaatt attgagttcc tcagctagag
      601 cctttgcatt tttgatggtt tgcgccgcaa agtcttttcc gaattctaat gcttctgcaa
      661 aagtaacagc ctttgccgcc atatgatgaa tgtgatgaga actggtaaga cctggaaacg
      721 tcgccttttt gatgacctcg gcgtttttct caaaagccaa aaccaagcct ccctgaggac
      781 caaataacgt cttatgggtg ctcattgtca tggtatccac accttctctc aaagggtcct
      841 gaaactcgcc acctgctatc aatcccgcca catgggcagc atcataattg atgtggatat
      901 catacccttt tagaaaatca gccaattcct tgactgggtg aggaaacaag aatacagagc
      961 ctccaaacat ggctattttt ggtagcctat cttggctctt tagatcttca acctttgctt
     1021 tcgtcttatc aacatctaga ttcatttcct cactatcaaa ggcgaaaaac tcaatatcca
     1081 aaccatgtac aagtccagca gttccggaat gctctttttt gccgtgtgaa atgtgaccgc
     1141 cagcagggat agatggtgca atcatcacat ctcccggatt tgagaaagca gagtaaatgg
     1201 ctagatttgc aacaacccct gaaattggtc ttacatcagc aaactcagcc ccaaacagtt
     1261 ttttggcaag attcatgcat tgaatttcga cttcatcaat gtaggtacat ccagcataga
     1321 cccgttctcc aggccagcct tcggcatatc tatttccaaa atcagatatt attgcctccc
     1381 ttacggctgg gctaggcacg ttttcactgg caatcagagg tatcgaatcg ttaaaccatt
     1441 gattatgttt ctctaaactc gcgaaaattc gttcataagc ctctttattt gatgaatcag
     1501 tcataatatc cacagtcatc ttctacatta aaaaatacct atcggatttg ctcatatgat
     1561 atagtatata aaaaggggaa atggattcga tctcattatg gcagttcaag ctacacaatc
     1621 aaacatgcca gttgtaatac taaaagacgg tgcatcacaa agcaaagggc tagaagcaca
     1681 gaagaacaat atttcagcag ctaaaattat cgctgaaatt gtacgtacaa gtctgggtcc
     1741 aagaggaatg gataagatgc ttgttgactc attgggtgat gtaacaataa caaacgatgg
     1801 cgctactatt ctcaaagaaa ttgatgttca acatccagca gctaagatgt tggttgaaat
     1861 ttcaaaaaca acagataatg aagtaggtga tggcactaca tctgcagtag ttttagccgg
     1921 ttcgttatta gaacaagctg aatcactttt gctacaggaa gttcacccaa ccataattgt
     1981 agatggttat agaaaggcag ctgtaaaagc aaaacaatac ctaaaggata ttgcagaaca
     2041 agttactgct aacgacaaat caatcttatc aaaaattgca aatacatcaa tgcaaacaaa
     2101 attggtaaaa agagactcct cccaattatc cgacatggtt gtaagatcta ttaccgctgt
     2161 tgcagaaaaa caaggtgaag attatactgt agatattgat gatatcaaag tagaaaagaa
     2221 ggctggcggc tctatctcag attcgacaat tattcaaggt atcgtgctgg acaaagaagt
     2281 tgtccattct ggaatgccaa aaaagataac tgaagcaaaa attgctctca ttaacgctgc
     2341 attagaaatc gaaaaaacag aaactgatgc taagataaac atctcaaacc cacaacaaat
     2401 gaaatcattc cttgacgaag aaaataagat gttaaaaaat atggttgaca aagttgtagg
     2461 ttccggtgca actgttgtat tttgccaaaa gggagttgat gatatggctc agcattattt
     2521 agcaaaagca ggaattcttg ctgttagaag agtaaaagaa agtgacttga caaaactatc
     2581 aaaagctact ggcggtagaa tagtatcaag ttttgatgat ttatttgaaa aggatttagg
     2641 aacagctgga ttagttgaag agcgaaaaat cgaagaggat agatgggtat tcatcgaggg
     2701 atgcaaacat ccaaaatctg taactcttct gttaagagct ggttcacaaa gagttgttga
     2761 cgaagttgaa agatctgttc acgacgcact tatggtagta aaggacgtta tggaattacc
     2821 atcaatagta gcaggtggcg gtgcacctga aacatatgct gcaaccaaga ttagaaattg
     2881 ggcaaaatca cttgaaggcc gtgagcaact agctgcggaa aaatttgcag acggcttaga
     2941 atctatcgca ctttcactta gtgaaaatgc aggaatggac ccaattgata ctttagcttc
     3001 acttcgttca aggcaattaa aaggcgacaa atggaccgga attgatgtca taaaaggcaa
     3061 ggttctcaat atgaaaaccg gcgatgttgt agaacctttg gctgttaaaa atcaaataat
     3121 ttcagctgca actgaagcag catgtatgct tttacgtatt gatgatgtaa ttgcaaccgc
     3181 aaaatctgca ggacccccac caggcgctga aggtggtgga atgccaccag gaatgggtgg
     3241 aatgggtgga atgccaccag gaatgggtat gccagatatg ggcggaatga tgtaaaattc
     3301 gtgtctaatc ccataaatca agtatttcta aaaacgtagt aactcatttt caccattctg
     3361 aaatcttata cacattctag aatgtgttgc agaaacgtct aaaaattgca ttttgtgcgt
     3421 aagaaatgac ttttaatctt tcaatgattt tagaagtggt aaaaacagcc ctctaggcac
     3481 aacatacgct ttaagggagc gtatgtatag gagtattcta taagccactt cggttcaaaa
     3541 tcaccatttt acgcctgcta cttcaacatc ttgtggtttt aaaataacaa aaactttaga
     3601 tggtaatttc taataaccat cgatgatgtg ggatttagat gactaaatgg aaacgtatga
     3661 gaagacgaca ggaacgaaaa agaatgcagg aagaggaacg aaaacaacct actgaacaag
     3721 aaccaacact tgtggaggaa acacctgaga acgttcaaac tttcccaaaa aacgaaagca
     3781 tagaggccgt aaccaatact ccaaaattcg ttatttcagg tgtaaaatac atctacttga
     3841 tagtatgttt tgctctatta tctggaatct tttatccttt agttactggt gacggatggg
     3901 aactgtcact aaaaggcatc ggtgttttag ctgttgggct ttttggagga atattactat
     3961 acaaatcaat aacgtcagaa aaaaggcgcg gtgtgttggt gtgttgtgga tttgggctaa
     4021 tcgcagttag tttgataatt atatacgaac ttattggctc gccactatac caataaatca
     4081 cacatcaaag taaagataaa actcgtgtgg gtgcggtcta atcgaaattt ctttttggtc
     4141 tcgacgctcc aagtctatta ttctattaat gactttagga gaaaaaatcg actcgaggaa
     4201 ttttttatca ctttcaagct catcaagagc ttcacccaaa tttgccggca gaacatcaat
     4261 tccatgtttt gttcgttctg atttcgacat cttgtaaata tctgctttga tcggatctcc
     4321 aggctctgtt ttatttttaa ttccatccaa accagctgct agcacagcag ggaaaacaag
     4381 ataaggattt gatgatggat caggaactct atactctaat cttttaagat acgaatattt
     4441 ttcaccttta aaatgtgcgg gaattctgat ggtggcagat ctattacctg tactccaagc
     4501 aatatagacc ggtgcttcat atccaggaac taatctgtga taagaattgg ttgtaggatt
     4561 tgtaattgct gttaatgccc tggcatggtt aatcactcca ccacaaaaat aacggcctgt
     4621 ttggctaagc tcatccttat catctacatc aaaaaatgca ttttctttat ttttccacaa
     4681 acttacattg gtgtgcattc cagaaccgga atccattgaa attggttttg gcatcattgt
     4741 agcaattttg ccaaattgtt gtgcaacatt acgtacaaca tatttgtaag tttgagcgcc
     4801 atctgcggcg tttgtaagga aatcatattt gatatcaatc tcacattgtc cagctgtggc
     4861 aacttcgtga tgatgattat cacaaattat tccaaaatta tcatttagta cactcacaca
     4921 ctcatttctg taaggtgtta aagtatcaga tggagtactt gggtaatatc cttcttgaag
     4981 acccatagga tatccattac catcttgatt ccatggggct tccttcgatt caatagagta
     5041 tgattgtccc ttgtatggtg ttaaaacatc ccaatgaatt ttatcaaaca caaaaaattc
     5101 tacttctggt ccccacaaac tataatcaaa tccttgtgtc tcgaggaatt tttctgcgtt
     5161 ttgactaatc cctctggggt catactccaa cctacctcta tctcctcccc aataaacatc
     5221 acaaagtagt cgtgctgttt tcttatcaga tgcccaaggt atgagagagt atgtagaagg
     5281 gtctggtttc agaataagat cagaatcctc tattgaagca aatccgacaa ttgatgaacc
     5341 atctagtttt ggcagaccat ctgccatttg gtcttgggta aaggtattgg ctgaaattgt
     5401 agtatggtgg aatcggccag tgagtccagt aaactgtaaa tcaataaaag aaacctcatc
     5461 ctgattgatt tttgcaaaaa cttcctcagg agaatatttg acatgagttg cttttccccc
     5521 tatcatttta tatggcattt acttatctat cagctaacac aatgaatcca acatttaagg
     5581 attattagga gaaaacagat gtctgaacaa aaccaaatta atgtgaactc tcttcattca
     5641 attgtattac aggaatatga aaacacgtca attcaaaaaa ttgactcgac tctttacagt
     5701 tctgtttcaa gattaattgg aaacctaaaa agtcaagaat atgatggtca tgaagcgaaa
     5761 atcaagacag ccttggttga tatgctcacc gaactaactt catctcttct taaaacacgg
     5821 ttagaaaaag caattttaaa caacacagat cattctgcat tgttagatga agaaaaatac
     5881 attctgaact ctcaggaaga aatgatagaa agaaaggagg taattctttc tggaattctt
     5941 aatggaaaag ccaaattatt agagtcaatt gtacaaaata ataaaacaaa gctaattgca
     6001 gtacgttttc ttgaggaagt agaagaaata tctggtgcag atgcaaaaaa atatggccct
     6061 tacaaaccag aagatatagc aacattacca tacgaaaacg ctcaaaattt aatatcaaaa
     6121 aattctgctg taaaaattca ttgggaaaac caatagaaat tataatcgat aaacatagaa
     6181 ccaatatgtc acacatcgac gtagatatct ttgaattttt gatacttaca attattccag
     6241 tcggggcact tttcatcatt gaaataattt ttcgactcag aaaatctaca tcatggttaa
     6301 aactaacaat tcaaggaatt actatggtta tttttggtgt tggatatttg actttgatta
     6361 ctccgcacgt actaactgca ttatgtttac ttgcattatc aggcgcctta ttttatcaag
     6421 ccagacgtgc aaaaatcaat ccaaaaaagt cactttatta atcaaccaaa tcttcctagc
     6481 atttttctaa acgcattctt tcttccagtt ccaccatcac gaataacttt acctttttca
     6541 ccaaatcttt ttgctcttat ttgagaaagc ttcacacacc tatgatcctc tggtagtcta
     6601 tgttcagaac aaaatggatc tttgcagtaa ttacattcaa atggcatgtc agtaatatcg
     6661 ccacagtatg cacatttttc tctaagcatt ttactcggtc accaattgtt tgtccttaac
     6721 gtattttaca aaactactcc aatccacctt catgtatttt ataggatctt ttaatggata
     6781 ttttacccaa tcttgtacaa ttgaaaactg atatttttta ctttgttcat cttcaagaat
     6841 ttccaacaat aatacacttc cccacacttt ttcctttaca ccaatatcta aaatattatt
     6901 aaaactaacc tcctgatttt ttttattttc taaatctgtt ctaagatgtt cttccgtgta
     6961 accatcaatc ataaccataa catctttgtt ttggataaat tttacaactt ggcgttgtgt
     7021 attggcaccc caccacttca tttttgattc ggttttttta ataaacatga gatgcttatc
     7081 ggttaaaatt aacataatct cagctccacg cccactaaaa aactctctag attctttcca
     7141 aactgaaaga atatgagcct gtattttttc tccttcttcc atagttagaa ataattctga
     7201 cttgataaaa actaatcctg taatggcttt tattaaagta gaaaaaacca cgtaatgtga
     7261 atttgaaagt attactagcg tttgcaatag tatctgcact aatacttcca atagcagcat
     7321 caggtcaagt tgagtctcaa gaacttgatc aatctcaatc atccaattgg gatgatcgtg
     7381 aagaactgaa attattacgt gttataggag gacctgactc agaactattt tcagaaccta
     7441 cagatattgc ggtaggtgaa tctggaaata tttacgttgc agattatgga aacaaaagaa
     7501 ttcagatact aaaccctctt gggcaacttg tacaaacaat agaactgaaa gggtctccac
     7561 atggtataac tttggacaag aatgaaaata tctatgtcac ggagtggtgg gattttatag
     7621 gtattgaaaa gtttacaaaa actggtgaac ctgcaactga ttttcagata gaagatcaaa
     7681 gtgtttttgg gctcccatca gatatagtaa ttgatccaga cggaatatta tacgtattag
     7741 agcaccgaaa tcttgacatt gattatggag aaaatgcagg agttcataaa atagatacgg
     7801 atggaagtta catagagttt atcccaattc ctgaaacagc aataactgat aaatcaaaat
     7861 tttcgttaat ggtgatggac catgagggtt ggctatacat agtagaccag tcaggtgata
     7921 atattatact attagacagt acaaacggta atggaagacc actggcctta atctatttcc
     7981 aaacccctac atctatagca attaatcctg acggctattt tttcatatct gatcaaagat
     8041 atgaaaataa taaaagatca attcatattt ttgatgagta ctatttaccg attagaacaa
     8101 taggcgatgc agggaaagaa gaaggaaaaa tttggggtat acatggatta gagtttgata
     8161 aagatggtaa catgtatctt cttgattttg agcaaaataa aattcaagta tttcatatag
     8221 ctcctaaagt gtttggacca ctattttatg aagaaacaga gaaagttact ccggtagctc
     8281 catcatactc tccatctggc gatgatttgg tagctgttct taatacaggt agtggacaaa
     8341 ttgtaataga attcttcgat gaagacgctc caaaacatgt agaaaatttt atagctttag
     8401 ctgaaaatga ttggtatgaa accacacttt ttcatcgtat cattaaagat tttatgattc
     8461 aaggtggtga tccaaatact aaaccagagc ctggaaatac ttcagaaaaa tggggcactg
     8521 gagatcctgg atatagtata gatgctgaat tcaatagtat aaaacatgaa agaggtattg
     8581 tttcgatggc acgatcaagc gatcccaata gtgccggttc acagtttttt atagttcata
     8641 aagactccac acatctcgat gagcaatata ctgtgtttgg tagattaatc acatcagaaa
     8701 gttatgaaat attagataaa atagcaaatc tgcaagtaaa ttctaggaac caaccatatg
     8761 aaggtgctat gcaacaagcg gtactttttg atgttaagat tgtttcaagg tcatctatag
     8821 aaaacgtact aactatggat ccaccaaata gaatacttgg atttacacca gaccacaaat
     8881 acattgatga agaattaaat ttttctgtaa cttctcctgt gggttggggt ataattaaac
     8941 ccgccaaatt tgacgacaac agtccaattt accttgcttc aggtccaagt aaggacggag
     9001 taaaaccaca aatctacatg aatagtaaac agttaggcga agatggtttc aaggatttta
     9061 tagataaacg cataacaaca tttcacaaac tccatgatga aggaaaacta gacttggaaa
     9121 cagagaatct gacggaattt atgggagggc atgcatatgt agttaccttg aaacaataca
     9181 taaacaaaga tcttgtaggt aacgaaccat accaagcaca attaaagcaa tttattttcg
     9241 ccacctcaac caccgtttac ggaattacat atgtaaacct tgaaagtaat tatcctgatc
     9301 atttagattt actaaatgag ttcagatatt cctttctggt agaaggtgaa acgatgcaga
     9361 ctctgggtgg agtagttata ggagacagta attttgatgg taaatattac aacaacgaag
     9421 aaaaacagtt tagatttgag gtaccatata gatgggaacc tttgactgta aaaactggag
     9481 taggtgatga tggtgttggg tattacagac tatcattaga acccaatgca ttacatgtaa
     9541 attatttcaa agatggagtt caagcgcgat atatgataaa catttacgag actactgacc
     9601 acgcttttac tgagcataca acacagatga agaatattta tcatggttat gaaaatgaag
     9661 gcaaactaag tttggcagat gaatatatgg gtatgacagc agacggtcat ccagtatatt
     9721 ttgttgaata tcttgaacct tattacatag ctgaagatga atatattccc attcatacaa
     9781 gagaagtaac ctttgcaaac gctgactttc tatacaaaat cacctataca aaccatgaaa
     9841 ataatttcac acgggaaatc agttcataca attacctgct tgaaaacttc aaatttaatt
     9901 ttgacggaaa aatgaaatct gttgattggg gtccaaatat tataaaatat aatccggtgg
     9961 atgaccgaga cccatcagaa agaggtggtg gatgtttaat tgcaacagca gcttttggtt
    10021 ctgagatgtc accacaagtt cagttcctaa gggagattcg tgataatact gtacttcaaa
    10081 cagaatccgg tacattattc atggctggat ttaaccagtt ttactattca ttttcgccaa
    10141 taatagctga ttatgaaagg gaaaatcctg tgtttaagga ggctgtcaaa ttgacattaa
    10201 ctcccctatt gacgtcactg acattattgc aatacactag cattgactcc gagtctgaga
    10261 tgcttggata tggtataggc ataatcctat tgaacattgg aatgtatttc gtggcaccag
    10321 cagttttgat aatgaaaata agaacaagtt acaataaaat acccaaaaat atctaatgag
    10381 accatgaaga aggagtacgt tgtagttcgt atagatgccg caccagatgc agcaccatac
    10441 gtggtagttt ctttgtcatc aaagaaagat atgcaggaaa gtgacggacc actatcatct
    10501 ccatttggaa agccaaatgt tatgggtttt accaacatga atgatatggc taaagagcta
    10561 aacaagatga tggcaggtgg aatgccagga atgatgggag gtaacacaac atcactaaaa
    10621 ctagacatga tggagtacaa agatctaggt ttgtctgtag gtgataaagt gtatttggaa
    10681 ctatcaaagg cagaaaattc cggcgtttaa acaactcata atttaataat ttactatatg
    10741 gtttctacaa tagggaccgt cgtctagatt ggctatgata ccagcctggg gtgctggagg
    10801 tcgcaggttc aaatcctgcc ggtcccatca taaatttaaa acctttaaca aatctagaaa
    10861 attttccttg gtttgatcag ttttatactt ccgtaaaaca gaaatgattt tagattttct
    10921 agacggctta caaagaggat ttacaatttt tgaaatgcct gaacctatgt aaaatgcctg
    10981 agtagcagat gttacatttg aaacccttct atcaacactt gaactttcaa aatcaagaat
    11041 agtaattttc ttaccaacaa tcacatgttt tgttatgtta cttagttctc catggtctaa
    11101 accaatttta tctaacttgt agcaatccgt aagagttttt ttaattatct tttttaattg
    11161 tgcaacattt ggcttttctt ttaatttgtt tatccacttt ccaatttttt caccatcaag
    11221 atattctatc acaagaaaat ttttactaaa acctaataat tttggtccta cattaatttg
    11281 gtttgttatt gtaagaaatt cggcttcttt ttttagattt tttcgttggg aatcaatccg
    11341 tcttattttt acagctactt cctttttgcc tattttacat aaaactacga ttccaacata
    11401 tcccttacct aacacattga tggtatcaat tttcaattct ccttgaaatg aaacactttc
    11461 tacaccaaga gattctaact ctttaattcg agactttatc tgcgcctttg ttccccttgg
    11521 ataaacccaa atgtttgagt gaggcggttc agttatctca ttaattttaa gaaaagattg
    11581 tttcatctgt tacggcaatg tttctgattg cctcattaat ggatttgttt gataccttat
    11641 ttgcaggcac aacttttact ccctttttga aatccttctc aagcccctta ggaacgcccg
    11701 aattttttag gttggttttc aaaagattat tcaggaataa tattacatca gaatacttcc
    11761 ttttttgtaa tgattgtatt ttattttcct cattaatcca cataagctca ctccttttca
    11821 aattagctga aatgaatttt tcacaatgat cttcactaga aatatttgga cccactcgta
    11881 caaaactctc ttcaattttt tttgtctgta gtagaaatat caacgcgcca ttttcctttt
    11941 cgtcttttgc agatgaattt cttaaaactg tgaatccatc aagctctaat tgagttttta
    12001 gtgcatttga tgcacgtttt atctgtcccc agatgatatc atcacttttc tgtttaaatt
    12061 tgaattgaac aaccacaatt ttatcaatat tcttcatatt acgcttaggt gttggttcat
    12121 taaaaaattt cttggaaggt ttttttagaa actctctact tgctaaaaca aactttccta
    12181 gactttctac cgaaatagca gcaccaagat ttctattact atcaatcgga tcaattatca
    12241 caatagacga actgaatttt tttgcagatt tacctatcac atctccttgc ttcaatttcg
    12301 agattttctt caatgttttt tcaaaactac caaagtatga aattagtacc tcagatacat
    12361 acccactaaa tccttctttt gccagctctg caccataaac atctatgtga aggaaaaact
    12421 tcttaagaat tctaacttca tctttcatag agttagttag ttttttcgac atgaattttg
    12481 tatgatgtat agatctatct gtagcgcttt gccattctcc tacatttaca tcataacaag
    12541 gtacaacatt tactttagtt ccgttaacta cagcttcgac aaaaggatgt tctgcatatc
    12601 tagtatatgg tttgtatttc ttcaaagatt caaaaccaat ttttgttcca atacttctaa
    12661 aatccttttc agatgttttt ttattaaatt taacaaaaac atcaatgtca gctttttctg
    12721 gtaaccatgt atcctttgca tacgaaccac ccagagcaaa accaactatt tgattatatt
    12781 ttccaataga ctcatttatc aaatcacaca ctttttttgc aatctgttgt ttttcttcct
    12841 gcaatctttg tgaaggaaca gttatttttt ttgcgtaatt taatatggga ttcatttcct
    12901 tcctttcaca accaagagat ctgtataaat cggaccgttt ggggtgagtt cgctcttctt
    12961 tagtttaacc tcagaaatca tctgttttcc aaaataataa cctgagagtt tttctaactc
    13021 atttgttatg tctccaattt tatttttaat tctgaatatt gtaacatgtg gcttaaattt
    13081 tttatctggg ctaaatccga aacacgataa ttttttctgt atatcaacca ctaattcttc
    13141 taattcactc acaccatcat ttattccaac ccaaaccact cttggattat taggattagg
    13201 gaaaactcca acacctgaaa aacttatcga aaaaggagta aattctaaat cacaaagatc
    13261 tctagaaatt ttttcaacca tctgatcaga aacttcaccc aaaaactgta cagtgaaatg
    13321 caaattctga atttttactg gtttggcatt tatttttaat tcagactgaa tcttactgat
    13381 agaatctaaa acatttttgt cgtttatttc aattgcaaca aaaattcgca ttatgctttt
    13441 ttattattgt gtttttaatt actttcgtaa acttcatacg ctggcattga taaataattt
    13501 catattggta aaactcatag tctgtcttac tggcatgcct ggttctggca agtcaacaat
    13561 cgtatcagta ttaaaatcaa aaggtattga aaccctaaat ttgggtgatg gtgttcgcgc
    13621 agaagcaaaa aggcgtaatt tagaaccatc tggtgagaat cttggtaaga taatgttaga
    13681 gctgcgtgaa aaaaacggac ctggtgcaat agcaaattta cttaccgatc aaattcagaa
    13741 ttcacaatct aacataatca ttattgatgg tattagatca atagcagaaa ttgaagtttt
    13801 aaaaaatgtt ggatctgtaa aattgttatc tatagaagca acagcagata ctcgctttaa
    13861 atttttgaag gcgcgtgggc gttcagatga tcccgaaaca aaggaaaaat ttgcagaacg
    13921 tgataatagg gagatcagcg tgggaattga caaatccata tcaattgcag atgaaaccat
    13981 ttctaataat gatcttacat tggacgaact tactgaactc gcacacacag taatcaaaaa
    14041 atggattgat aattgacacc actaaacgtt gattgtaaaa tatctgcata ttgtatgata
    14101 aacatatcag aggatgttaa caaaatacaa acagctatat cgaatgttct cactgatatg
    14161 gatggaaggg tgtctggaaa ctctttcatc tccaattcaa acaattatga atcattaaca
    14221 aagatctatg aaactatacg aggaaacaac agtcaaaata cataccgacg tcatctgaga
    14281 gctaatattg ataaaaactc tacctggttt tatctaaaca aacaggcagc ttttgtagat
    14341 gttatagcca tttgtaacga agcagatgaa tcaccattag gtccaataaa aatagtgtta
    14401 cattctgaaa acattgaaga tgtgatagaa tggttagttt ctgatactga ataatcagaa
    14461 ttaatcactg ttcacatcct cttcacttgg atagtatatt cccattacta gcaagtccgt
    14521 aactgtttag ttaaacattt tattttttat tcaactaatc ttaattttta attgatcatt
    14581 agcagctatt acagaactat gaattgactt tgcttcttcc aacaacactt tatcatcatt
    14641 atatctagca gaaaagtgag taagaatcaa atttaacacc ttagcatttt gtgcaagtgt
    14701 cgctgcttct tttgcagtag aatgaccaga ctccaccgcc ttttctttca attcatctga
    14761 aaaagtagaa tcaaagatca aataatcaca ggcattgaaa aactcctcca actcttttgt
    14821 tggtcgagta tcaccggaaa cgccaatttt cttaccagat atgtttggtc ccattacttg
    14881 tgaaggtttt attgttttat tttcaattgt aacttcctcg ccacattgta acgtatgcca
    14941 taactttccc tttggaactc caagggcaat tgccttatca ggataaaatt ctccaggctt
    15001 ttctttttcc ttgaaaagat aagaaaatgc agtaacagaa tgctcagctt tacaagcata
    15061 cacttcgtat ttctcttcgt ttacaaccaa tccattattt actgaagtta ttaaaactgg
    15121 aaacgacaag ccaaaattca taactttcat gtttgttgca ataaattcct caatcccatc
    15181 aggtccgtaa atctctattg gttcagttct attttgtaat gtcattgttt gtaaaaatcc
    15241 taaaatccca atacaatggt ctccatgtaa atgcgtgaca aatattttca tttttttatt
    15301 ccaaccaagg cctgatttga ggaaagaaat ttgtgcattt tctcctgcat caaacatcat
    15361 gatttcgcct tccctgatta aacacataca tgttgtatta cgctcaagtg taggctgcgc
    15421 ccctccagtt cccagaaaaa taatttccat cattattgaa cagcttgtgc tatcactcca
    15481 gacacgtcaa cctttgagat acttctagga attgtatttg tacttatgat ttgtgtcact
    15541 ccagcctttt tgatattttt ctctgcatcg cctattagta atccatgtgt gcaaacaaca
    15601 aaaattcgtt tgcatttttg tttttttaga aattgagtgg ctttaacaac actgccacca
    15661 gtacttatta tatcatcaac tattatcaaa tccttcccat taacatcagc cttggttgat
    15721 cgaatactca ccttaccagt ttttctattt ctggattttt tcaacgttat agaatcagat
    15781 tttaatatat ctgcaaattt ttttgcacgt aaagaaccgc ctgtatcagg ggatacaact
    15841 attggatttt tcaattccag tttttttaaa tatttggcaa gttctggtat tgctgaaaca
    15901 ttttctgtag gaattttaaa atgacgtaat gcggttttgc tatgtatgtc tacagcaata
    15961 atcttttttg cgccagctgc ctgtagcatc tttgcaacta cagacattgt gactatctcc
    16021 ccactaagaa actgcctgtc ttgtcttgca taacccatgt aaggcattat tgcataaatc
    16081 tttgaggaga ttttcttggc ttcagaaatt attgataata tttgtaatag gtttgtatca
    16141 acaggtgggt atgttgattg aactaccaac accacacttt tcttcaaatt atgtctaaaa
    16201 gtaattttac tctcaccatc tggaaaagtt cttgtatctg catctacgta ttttgccttt
    16261 aatcgtctgg caatacgctt ggccaaacct ttagacgcat tacctccaat tacagtaaag
    16321 tttaccaaca ttagatcgaa ctaaaacaga ttcttaaatc ttacagaatc tattcgtcac
    16381 cctcaccgcg tgccaaatca cccataccaa attcatcaac cgcttgctta cgctgatctt
    16441 caaattcaga aatctcttgt gattctctct tttcatctcc ctggtaaact gttctaattg
    16501 gccatggaat tctaatatca tattttttaa attcttcata cataataaga cgcatagcac
    16561 ttttcatttg gaattgtgcg ccatacgaat taacataaac ccaaagagaa aagtcaagtg
    16621 aagaatcatt gaatttgtta aaacgtacag ttggttgatc tacatcaacg tgaatatctt
    16681 tgtcacatcc acaacttggt ttattttgat cgaggtaagg acaacgtttt tgcgcagcaa
    16741 catgactacc ttttgaattt tttacttcaa ttagtgcacg ttttcctatc ttaaccaaga
    16801 tagatgcgac ctgttttggg tcattaagat atgagacacc aacatctaca acagctggta
    16861 ctaactgaat ttcttttgtg aaattaatta tttctgcagt aactaactgt cttgtaggaa
    16921 ttattgctaa tgattcgtta agaccatgtc taatgtaagt aactcttgga gttattctgt
    16981 gtacataacc attgtaacca ctttctagtt gaactctgtc tccttcagta aacactttat
    17041 cttttctaat tagaatatat gcaaaataat tttctaatgt ttcttttaat gctaatccaa
    17101 caccgagcgc cataccacca gtagcagtag ctaaaacgaa aagatcaaca ccccaccatc
    17161 caaccacacc gataacagtg gcaacaaaaa taccaatagg aagaacttgc ttagctgatt
    17221 tgggaacacg ttcttcttgt aattgctgat ggccagctgg ttttgatgca ggaattattg
    17281 gatcatatcc tttttgtctt ctcacttctc tccatacatc aacagcgtag atttcttgag
    17341 gttttgtacc aagtacaagt tttcttcctg tttcattatt cccactcctg caatcattaa
    17401 gatacttttg atattttttc ctctctgcct ccttccaatc ttcaatagga tccttcaccg
    17461 tcttttcata acttcctatt gtaacgccct tgcttgttct ataattttct aagaaattaa
    17521 gcccctcttt tgtttgcaga tttttttcaa aatcttccat tgtcaaatct tctggaggag
    17581 tttttggtgg aatccatttg aaaattttat gaaacaatcc gtcctcatca tccttaaaac
    17641 ccttcatatc tttccatttt tcaaaatctt ctgcttcgag ttttgttttt tctgctttat
    17701 tcaatccaat tggtattaga tgagaaactg tgtagccaat aacaagtatg ttaattgtgt
    17761 ttagtatctt tgcaaatgtt tcgagtgctt ctactgagct agggttaact gcatctgtct
    17821 ccgtaaactg gatgaataaa tttattgaag taactaatcc aattgcaaaa acagacagca
    17881 tcgcagtacg ggcaaacctt gctacatgtg gacgtgtata actgaattct ttactggaca
    17941 tccatttacc aaaacctctg tatgatgatg cgagtataac caaaccgata accagaactc
    18001 ccatcagaat ctgaagagat gtatctgatg ctaatagatc cgaaacagtc tgaaattccc
    18061 ctgccgaaaa ttcactaaga tcgtcaacca tttcccctat tcacgaataa aattgcctaa
    18121 atgtcagagt tatactcaat cagtcagtag ttattcaaac aaatatcaaa attttttata
    18181 aatatttacg cctattttgc cattttgagc caagatttaa caatagtatt tgataaaata
    18241 tgtaatgaca gagcagcaaa caacgtggtc aataaacgaa accacaccga ttaaatctta
    18301 cactttatca aattttcgca ccattccaca aatccaacag atgtcagagg aagcacagtt
    18361 tgagatggag gtcgttggta atgtactacc ttttaagacg aataattatg tcgttgagca
    18421 acttattgac tggaataata ttccaaatga tcctatgttt gttcttacat ttcctcaaaa
    18481 aggtatgtta attcctgaac attatgataa gatggcaagc gccctaaaga gtggagcgga
    18541 tagaaaggag attcaaaaag ttgcaaatga tatacgctta aaattaaatc cacacccagc
    18601 cggtcagatg gaacttaata ttccacagtt aaaggatgga acaaaacttt atggaatgca
    18661 gcacaaatat gacgaaactt gtcttttctt tccaagccaa agccaaactt gccacgctta
    18721 ctgtagtttc tgtttccgat ggcctcagtt tgttggtatg gatgagatga aatttgcaat
    18781 gaaagaggga gaacaactag tacaatatct gagagagcat ccagagataa ctgatgtttt
    18841 gtttactggt ggagatccta tgattatgaa ggctagcatg ttttctgttt atactgatgc
    18901 cttactagac gccaaattac ccaaccttaa gacaatcaga attggaacta aggctgtatc
    18961 gtattggcct tacaaatttt taaccgattc tgatgctgat gaaacactga agaattttga
    19021 aaaaatagta aaaagtggaa tacacttagc gataatggct cattttaatc accttgttga
    19081 actttcgacg gatccaataa aggaagcaat caaacgcata agaaatactg gtgcacaaat
    19141 tagaacgcaa tcaccacttt tggctcacat aaacgatgat gcagatatgt gggcaaagat
    19201 gtgggaaaaa caagtttcct tgggctgtat accttactac atgttcgttg ttagagatac
    19261 aggtgcccaa cattattttg aagtacctct agtaaaagca gaagaaatct ttcgtactgc
    19321 atttagaaaa gttagtggtc tagcaagaac tgttagagga ccaagtatgt cagctactcc
    19381 aggcaaggta cacgttcttg gtatatctga agctgctggt cagaaagtaa tgatattcca
    19441 gctattacaa ggaagaaacc caaaatgggt tggcattcca ttttttgcaa aatatgatga
    19501 aaatgcaata tggcttgatg atctagaacc cgcatttagc gaaaaattct tcttcgaaga
    19561 ataatcttaa ggtaaaatat acaaaaactg cctaaactac tataattcca tttttgatta
    19621 gatactgtat tccggaaacg aatgtattgt catcaattgc cccattagcc caccatcctg
    19681 cattattttt gatccaagca ggaacttctt gtgactctat agcctcagac gctgtaggtg
    19741 gtataaccat tattccttct ttgatcagaa actcaattcc ttgaagaaat gctgaatctg
    19801 gaatctcttc attagcccac catcctgcat tatttttgat ccaagcggga attgatattt
    19861 caccagtatc gattctgttt acaattaccg gaagactggc attagctaat tcattctcat
    19921 ctagattttc aaactgcaat attataggcc cgctagtatt ttcaggtaca agaaattcaa
    19981 ttgtattatg ttcggttctt aaatcggtac taataccatt tttttgtaaa agcttttttc
    20041 catcataaat aattgataga tcataattta ctgaaacagg tttatctaac aaaaatacat
    20101 caagaatatc aaagataaat gtagttttta ttcccgattc tatctttttt ggttcccaac
    20161 tcaaatttat tttaaactga ccattttctg taattgtact aagtaaattt tcatcttttg
    20221 gtgtaatatc aaatttaatc ttgtttgaat tttgtaattt ttgagacaac tctaatattt
    20281 ctttttgact tatcactaaa tgcataattc tattttcatc agaaaaatca tccacggtga
    20341 tatcactatc taatatatct aaaccattaa cttttgccga aaaacctttt accattaaat
    20401 ccccaaatgt ttttggaata agaagttctt gatgtattac tgatacctgt ttcatatttt
    20461 cttcagacca atcaaatggc atagagtagc taataattcg attctgttcg ttataattaa
    20521 aatcattttt aatttgttca taatatgtaa ctatacctaa tttttgttgt ccaaaattag
    20581 gatcatctat atcataatag gttgtatcag gaaaagatat tcctgcatca taatcaattg
    20641 gaggagaaag gaaatttgta tatccttcag ctgttgttat taatatttta aaattataca
    20701 agccaccaga atcaaatgtg tttccatata cattaacaat tttgttagga cctaatattg
    20761 aaccaaataa acctgcatcc tcttcattag ttatgatctg ttcagactcg gtaggaatta
    20821 atcttataac aaatatacca tcatctcttt gaccagttac atcaaataat gtttgatttt
    20881 gttttattgc catcacaaaa aatgtaacat cattaattgt tacaccatta gatgtatcaa
    20941 ataatgtaaa cgatatttcc cgcgcatcac tatcaggtat aggattagat gatatttcaa
    21001 gcgcaacttg tttagaaccg agatctactg gcggtaaaac ttcataaccc aaaccatgtc
    21061 catacgaatc tggaattaca aaaaatatgg ttaatgtcaa tataggcaaa acaaatatca
    21121 aaaattcttt atatcgcatg taaaatcacg ccttattgaa ttatttcagc atttgtgcct
    21181 gaaaaataaa caacctttag tatatggttt ataaagtcta tgagattaca tttacaatct
    21241 gacataaaat ggtaaataaa aataaaacaa aagaaaaatg cccaagatgt gctaagggta
    21301 cattagtcac tgatgttgaa tcgggagaaa tgttttgttc aaaatgtggg tttgtattaa
    21361 cagataaagt acaagaatct ggaccagaat ggagatcttt tactcaagac gaacacggtg
    21421 atcgagctag agctggagct cctacatcat taacaatgca tgatatggga cttgcgacaa
    21481 tcattaaccc tgctaacaaa gatgcagctg gaagaccatt aacatctaca atgaaaagta
    21541 ctattgaacg acttagaact tgggatagta gaagccaagt acatgaacct gttgatcgaa
    21601 atttcagaca ggcatttagt gaactaaaca gattaaaaga taaacttgct atttctgacg
    21661 ctgtaataga aaaagcagct tacatctata gaaaagcatt agacaaaggt ctggttagag
    21721 gaagatcaat ttccgctttg atggcatcag cattatatgc agcttgtaga gatactgaaa
    21781 ctccaagaaa tcttaaagat gtagaaaaag ttgcaaacat aaaaaggaaa gatattgcaa
    21841 gatgttatag attacttgta aaagaattag atctaaaaat gccagttaca gattctgttc
    21901 aatgtgtagc acgtatagca agtaggattg gtattcaaga aaaaacaaaa cgttatgcaa
    21961 caaaagtttt gcaagctgca caaaaaaatg aagtatctgc aggaaaagat cctatgggat
    22021 tagcagcagc cgcattatat cttgcatgtg taaaaaatgg tgaagataaa acacaaagag
    22081 atattgctga atcagccaat gtaacagaag ttacaattag gaatcgttac aaaggactga
    22141 aagaagcatt tacgatgtaa ttatttttcg ctctgaatat aaccaccatc ctgactttct
    22201 ggaggcggaa ttttttttgc accacccaaa ccaattgtgg taatgataac tgatgttata
    22261 acaacaaaga agattatctg tggatatgct tcagcatttg gaagtcccat tgataatgga
    22321 aatgtagcta atactgcagc agctaaacca cgtggaatca tcacagaagt caccttacga
    22381 tcaagtttag aaaaacctct aaccaaaact gtcttcgtta aaattattct accaatatag
    22441 attgcaattc cggctataac accaaaaatg acatattcta tttgtgtaaa gcttgctaac
    22501 aaacctacaa aaacaaagaa aaacgctctt accaagaaag tgacctggct atgcaaagaa
    22561 ttgtctatgg tatcttctgg aaatttgatt ttaagaattc gtgaaaagta tgtctttttt
    22621 ccaagcatta atccaaatac gagagctgtt aacgctcctg attcgccaaa agaagtggct
    22681 aagaaaaata gcataaagac catgccgatt gtaagcatgt agctatgctg tgcatttttc
    22741 agctttgata taacaaacat ccaaggaatg ccaacaccaa gacctagcac aagaccaact
    22801 gcaactgcct ttccaattgt tatccctaat agatcaaggc taaattctcc agataatact
    22861 gcctcaaata aaacgaatgc aatgatcaca gcaaaaatat cggtcaatgc tgactcgaac
    22921 ccaagcatgg atttggcatc ttcagaaatg tggatctttt taaccagacc aaaaacaatg
    22981 atagaactac tacctccaag tattgagcca agcaaaatac tgtctatcca ttcccaacct
    23041 aaaccataat gagcgagtcc agcaacaata ccaactgata ctgcaaaacc cacaattaca
    23101 agaattacag caaaatgtgc agttttcaag acttttccaa tatgaagatt taatccacca
    23161 tcaaacatga taacaattaa tgcaacagct gcaaaatagg ggacaatctg caatactgct
    23221 tctggttgaa ttattcccaa aatcggtcca ataattatac ctaaaaccat caaaaacaaa
    23281 atatctggaa tgcctgttct tttgaaaaac gcttctccta agacacccat tattattacc
    23341 acactggcag ccagaagcaa aattggtgct gatgcaacaa gagaatctcc aaatgacaca
    23401 tcagctataa tattttgtaa attcacaatt ccctgaagaa ggaaatttga atcaaattgt
    23461 tctgtgcttg cttcaggtat ggggtttttt tcaaaatacg actttagcat atctcttaca
    23521 ttagaaccag aaaaatcatt aaaatcaata tttgagaaaa caccatcctt gaaaaatgaa
    23581 acaagatcat ccaataaggc gaaaaagacc aaattcatac ctaatcaagc cattttgtcg
    23641 tttaaaataa gaatctgtca agctaccttg atttaatgat acgactagat ccatgctcat
    23701 ttatgaataa attataagct acgataccat attttgcatt attgagtgca acagacacgc
    23761 ttagggatga tcataaacag atcaaaagat tagataagat cgtttcaaaa tgttcttccg
    23821 atatttatgc tggtaaaacc atcccatttc atgatctaga aaagatcaca ttgatcattt
    23881 cagagttttt agattcgatt cattattcta gagaagagga ttcatacttt ccctgtgttg
    23941 ctagctatga tcatctcaaa aaagagattc gagcattgtt aatagagcat gaattcagcc
    24001 gaaacatagc ctacaaaatc actctccatc taaaacgttg gaaaaatgga gaggattcta
    24061 gtgagcctgt agcgcggttc ctaagaacct attcaattta tctaaatgac catatatcaa
    24121 aggaagagga tttttttgag cgggcagaaa aagagattat ttctaaggaa gaggaaaagg
    24181 acatgtttga acaatttcaa tcagatatgg ctatcgccaa aaaaatggag tctattttag
    24241 aggatataga ttatttggaa aatcagatgt ggtttaagaa ttaagcctaa gttgttaact
    24301 ctggttttaa tgtccaagca atacctagtg caataaatgg aattgatatt atacctataa
    24361 ttggcataag cccaattatt tgatcttggg taatatctgg tgaagtaaac aaccatggca
    24421 atggaagtgt agatatcaac cataatgatg caagcagaat tatgacattt cgcttatttt
    24481 ttttctgttt atccaaataa tcactttaat ttagtattga aaacatggct ttttatttca
    24541 ttggaatgag ttgaatactt acaggtcctt tcaaacttcc attcggatct atgataatct
    24601 taaccttttg tgattcgcca gaactaacat tcttctcacc caaatactcc caagtatacc
    24661 acgaagacat ttcatcctga actaaagttc cttgtaactc aaaatcttca tagaacgaaa
    24721 tggtttttcc catcaacttt atggatagtt tttttggact gtcaccattt ggcacgaatc
    24781 caaattgata agttccttct ttgacatcaa atacctcaac ataaactcca ttttcataca
    24841 aggccggatc agccaaagtg acatggaaga tatcagaatt tccaaactga atattatctg
    24901 aattatctgg gtttatctga gccccaacaa atatcacacc tatgatgact actagaataa
    24961 tacctccaat tattggtttt ttactcacag agttcgtgat gtttactagt aaatttagtc
    25021 atcgaatttt aattaactgt aaaccataat tcacacggtc tactaataac gaattttacc
    25081 acaaaatcac atgacttcgc tttcaagtaa atggtcaaag ccgcaaaagg ctggaatatc
    25141 tgaacgcatt aataatataa ttaaaccaag cggtgcatta aaaccacgag tagaaaccgc
    25201 cataaaaaaa ttacaggctc aagttgcaaa attagatgtc atgctctcat cattaaacag
    25261 tagagatgag aaaatttttc acagaattgt aacagcaaca caacaacatg atactcacac
    25321 ttctaaggca ttatcaaaag aactagctga agttcgtaaa gtacgtcgtg ttcttggtaa
    25381 tgcaagaatg gctatagaac aaattgaatt acgattaact acattccatg atttgggaga
    25441 tacagtaaca actgtgatgc caaccatagg attaatgaaa aacatgaaaa catcacttgc
    25501 caagtttatg cctggtgcag accaagagct taacaacatg gcagaaatgt taggtggttt
    25561 aatgactgaa acgttccata gcagtgatgc ttcatttgga gttgatacgg ctatgaattc
    25621 tgaatcagaa aaaatcttgc aagaagcagg cgcagttgca gaacaacagg ctggagataa
    25681 atttccctcc atgccagaag agatatcaga acgttcttct tcatcacgtt ttatgtgata
    25741 gataacttac tgattaacca gaattagatt ttgattcttt aaatctttta attttatctg
    25801 cttcatcagc caatatctta tcaatacctg aaattttatc acgttggccg taaattatat
    25861 tacccacttc atcggcttcc ttttctacac cagctctttt gttagttaat tcttctaacc
    25921 cattattttc atcttgtatg aattcgttta ttttactaag cttttctttt aaatttttaa
    25981 tatccactga ctcatcttca atatcttttt ccaatcttgt tcgtttgtct tttaggtttt
    26041 taatcatcaa acctacataa tcgatagcat cttccaattt tgctcgtttg tccatcaaag
    26101 atcctattcc aatctgtgat gatgatgaag gttcttcagc aggttgagaa tacgatgaag
    26161 gttcttcagc aggttgagag tatgatgaag gttcctgagc aggttcaggc cctcgttcag
    26221 aatcaaaatt cgagttttct tccattccag caaattttct cataaaatcg tcacggtttt
    26281 catcttcatc cttgtctttg aagatatcca agaaacccat tgatagaatg aattgattca
    26341 gaaagataag atctattatc agacataatt ggcaaacact ctaaatattt cgcccccgcg
    26401 caaaaaaacg tccattttag cagatttttc atccatttga ggcgttccgc tttgcgttcc
    26461 gctgtgatgt ttcataatgt ccctcataaa gctcattatg ttatcaaaag catgtcaaaa
    26521 caacagtaca gatccgaaat gggaatcatg ggtgatatct tggatgttac catggacgga
    26581 ggccaacagg gagtaattgt atctgcaatt tctcgaagag caaatctttc tcactattct
    26641 gtgttagata agtgcgaaaa actcattacc gctggcttaa tggaatccag aagaaatgaa
    26701 agaaataggc tattcaaaat aactgaaaaa ggtcttggtt tcatcaagga attccaacga
    26761 tttcaaacaa tcgtcgactc catgaatcta cggtattagg ctatgaactt gcaacaaatt
    26821 gttgcacaaa aacatcaatc tccggggggg gaaatgcttt tggtagggaa cacggatatt
    26881 ctgtcagcga tggtcaggat accaatagtg gtgataggtt tggtggttgt agcattgagc
    26941 ataccaatcc aacaatccct tggttctagt cgaacccttg attttactat ttattcagac
    27001 ggctctactc atgtttttta tgaattagaa actaatccgt tggaaccaga attttcaact
    27061 aaactttttg gaacaacact agaaaatctt acaattattg ataacaatgg tttcttatta
    27121 tcctatgaaa ctgtgggaga ttccgtttta attgaaacat taggtgcatc tcatatcttc
    27181 attgattatg atacacaaga actagtttct aaaactggaa aaatatggaa attttctgta
    27241 gatccatcct ctgaatactc gttactaatg ccaaacgact cggtcattgt tgagatgaat
    27301 aattttccta tcagtatgga ggtagaaaat gaacaatcac gtttagtttt taccccaggt
    27361 caaacaaaca ttagttatta cattagcaca ttagcttctg ctcaaaccac agaatcaatt
    27421 tctgaagacg gcggaactga taactcatca atcttatgga tattagtagg tgtaatatca
    27481 tctattgtaa ttgcagctgc aataattgtt ataaaaagaa aatctggaac cccaaaatca
    27541 tctgtaacca ttcagaaaga tgaagagcct aaactgcaaa gcctcgatcc cgaatcaatt
    27601 ttcaaggcaa aacaagatct gagagaggat gataaagaga ttattaattt tatttcagca
    27661 aatggaggac aagtttttga aagtgaatta cgaaaaaaat tccttcaacc acggacaacc
    27721 atgtggagag ctgtaaaaag attagaaaga catggaatta tagaaattgt aaagaaagat
    27781 tttcagaatg ttatcaaatt aagaagtaca ctggaggatg actcatgaaa aaattatttt
    27841 tctttttcct tttgtttttg attgcaagta tgactattgt tacaatacca cataatgtaa
    27901 gtgctgaatc acagcttgac atacttgtaa ggataactga acacacgaaa cagtatatca
    27961 agaatgatat gaacaaaata ggtaatattt ctaatgaaat ccaacagcta tatgataaag
    28021 gtcttaaaga aattgatcta ttaactgacg ctgcagaaaa tgaggatgct gtttcagcaa
    28081 aacaacactt tcatgctgct atgactacgt tcaaaaaaat tagaatagcg atttcaggac
    28141 tagaatcaga aactgtacca ttacaacata gtgctattca aagtcagacc attaaaaaat
    28201 atgaatggaa tatcaaaaaa ttaaaggatg taaacctgaa gttgaatgca aatatcaatt
    28261 ttgactctat agatggattg atgaatttag ctaagaaaaa tctgactgag ggagatattg
    28321 gacaaaacca acaaacgctt gagaaaattg cagacaaagg acgtgaaatt caaaaattat
    28381 tatatgatat aagtcaggaa ggcaaatttg acagagcgaa acaatttgcc atagaatata
    28441 ctaaaaatgt ggatcagtta atagaatcag caaaaaaaat gggtttatct gacactgtag
    28501 gaaaattaga aaattctaaa acaatcatta ttcatgcaaa taccacaaat cagatagtaa
    28561 atcagcataa aattatcatt attctgaaat ataaaatcga acaaacaaat tatttgcaac
    28621 aagcaaaact tttacaatta aaatcagtta tttccagtct tgaaaatcaa gccaaaatac
    28681 tagctgatca ggtaacagag gataacgcag caaaatattt ccttaaaaat attttttatc
    28741 taatagatga ggctaaaaaa gacttgaaaa attctcctga atcagtaccc ggaaaaatta
    28801 cagagataaa aaacctgctt ataaaaactg aaaaattgat taataattca tcataaactt
    28861 catatagatt ttcccagagt tgtggatatg gcctcgacaa aaattatagt atgtgttgga
    28921 atacctatga tcgttactgg ttttttgatt gcaattcttt gggcaccaac ggcttatggt
    28981 gttgaatcaa cagttgaatt tgtaggaagt ttaattggaa ttattggagt gatctttgtt
    29041 attgctgggt tattttatac aaaagaacca gtagcggcat aatcagataa tttactaatt
    29101 atcacgttaa taaataacaa aatttgtaat agataataat tgaaaactag actttttgaa
    29161 atttttactt caatagaggg agaggggata ctctatggaa ctaaaactct attcatcaga
    29221 tttgcaggat gcccattttc ttgtttttat tgtgatacac cagatgcgct tccatcagat
    29281 tctggcaaag aatattcaat atctgaagca tgtgacctaa tagataaaaa tctgcaaaat
    29341 aatacataca aagttaactt tactggaggt gaaccacttg tacagcacga atctgtcaac
    29401 gaacttgcta aacacataaa atcaaaagga attctcacat acctagagtc atcatgtttt
    29461 gattctcaaa aattctctta tgtcttacca tctatcgatt ttgtaaaaat tgaatttaag
    29521 acaaaggatt cagaaatggt tgatacaaaa cattacacgg atcttatgaa aaatacatta
    29581 gaatgtttac atacatctat aaaaatgaaa aaaataacat acatcaaaat agttgtaagt
    29641 tcaaaaacag aactggataa tttaaaaact cttttgaaac aaatttttaa aattgcatca
    29701 agagagacga tttctggatt cataatccag ccaacatatg gtgtatctga accttcatta
    29761 gaaaaattgt tggaattcta tgatttagtt tatccatatt atagggatgt gagagtcatt
    29821 cctcaattac aaaaaatcat agatgtccct taaatttaac cggataaact agtacagaaa
    29881 caagtcttaa aagatgaggt ataaatattc agtaatcttt tcccaatatt atatggatga
    29941 ggaaaaagta aaaaaactga ttagagaact gattatagaa attggcgaag acccaacaag
    30001 agaaggacta gctaaaaccc ctgaacgtat tgtgggtgtg tataaagaaa tttttaatgg
    30061 atacgaagct gattctgaat tatctgtaca attttcagag gattcagaaa ttgtgatagt
    30121 acgtgacatt caattttatt ccatgtgtga gcatcattta ttaccatttt ttgggaaaat
    30181 tcagatagta tattccccag acggaagagt ctttggaata tcaaaactgg tcagattagt
    30241 tgaaaaattt tctagaagat tacaaattca ggaaaggttg acgaaaaata tcgctgatga
    30301 attatatgct gaaggagtca aaggagttgc tgttttaacg gaggcagaac atctttgtat
    30361 gaagatgaga ggtgtacgaa ataacgcaaa tgtcacatct ggtgctttta gaggtattta
    30421 tgagcgtaaa gaagaaaagg aaagaataat agaattaatc aaaaatccta caaaatcctc
    30481 tttccaatga aaattattaa aagataaata atcaatttat cggattggat ctgtggtagg
    30541 acgtgataat gctcacattc caaaggaatc ttggccagct ttcacttggt atcttataga
    30601 atttgcaatt gtatttgctg ttgcaaacct aatcgcattt caaataatac ctatggttga
    30661 tggaatggta ggagaaaaga gtcttctaat ttgtgaacaa gaatcatgga aatctaattg
    30721 tgctccatca gaggaaaaaa tgtctgatac aggattacta aattggatat attggggtat
    30781 ggttggtgga gtgttcattg gctggtattt acttatacgt ggattcattc tgaaaaaacc
    30841 aattctcgga aatcgttaat cataacgata gactgtttta tcttcaatat tggcttctaa
    30901 aaatgccgct ttacgattat tacatgattc acatttacca cactgtactt tttcattact
    30961 atagcaactc caagtattga aaatattatc tcccaaaata ttatagcctt tttttaataa
    31021 tagttttttt gataattttg ctttgtatgg agtccaaatc tctatctttt ttctaatacc
    31081 tgatttgata ccatcaattt ctccttggtt aaatgacgat tcaatttttt ttgaaaagac
    31141 tggtctacaa tcaggataat ttttgtcacc catatgtgct ccatatgcaa ccaatgaagc
    31201 attgattgaa aaagcccagg cagttgcaat agataagaaa attccatttc tgattggtac
    31261 tacaattgaa taatcaaatt taccaggaat attttttttt gaacttgtca aaacattcgt
    31321 gtccccatac agatccttca tgaaatcaat gtttacaatt ttatgctttt ttaagcctaa
    31381 aatttttgca aacctttttg ctttttttat ttcttggttt gctttctgac cataaagaaa
    31441 agaaattccg tacatctcat attttgactt caaatacgca cacatactaa cagagtcaat
    31501 tccaccactg aaaactacta ccgctttttt cattttaaac cctaatctta ggacccatac
    31561 taggtttaca gatgattgta tcacaatcta tctttgctga actaaatcct ttctccatag
    31621 actttgcaac tttttttaca ttttgagatt ttttacagaa agctatgata gatggtccag
    31681 ccccgcttat tgttacacct aaagctcctg cacgaagtgc attgttcttt acctttgaaa
    31741 aacctggtat caagtgtttt ctagccggtt caacaattac atcctgaata gatcttccaa
    31801 taagatccga atctttgagc aaaaatccag agacgatgtt agcagcattt gacagatttt
    31861 ctgttaaatc tgataattta accgtctttg gaattacggc tcttgaaacc tgtgtctttt
    31921 tctttggtac ctttaatttt ggaattgcta cgcacaaaac caagtccttt ggaggttcta
    31981 gcctaatgac accaaatggt tttgttttaa ctaccacaaa tccaccaagt aatgatgctg
    32041 caacattatc ataatgaata gttccagcgc ttgctttttc ccctattcca gcatatttga
    32101 ctagtgtttt gttatctaat tttagattaa acagtttatt gaatgctagt gcagctgcag
    32161 cagcagaagc tgcactactg cccattccaa atccagctgg aacacctttc ttaattttca
    32221 tctcaatccc agattttatc ttgaattttt gtttcattga ttttattact aacccagctg
    32281 tgttttgttg tggatcttta ggtacatcat ctgtagttag tattctaatt ccatgccatg
    32341 gtttgtttgt agtggatttg ttagttttgc taagtgttat ttcgtcatga aatgcatcta
    32401 acgctagacc aaatacatca aagccaggac ccaaatttgc tgttgatgat ggcgcccgca
    32461 ccttaacact cgtaaccatt aatcttctac ctcttcaccc aagttaatta ttctactaat
    32521 ggcgctttct aaattaatca ttccctcttc agttacgttt gatattggga tcaactcatc
    32581 aacaaaatta ccgagattta atgatcttat tatatccata ctcaaagaat acatttctcc
    32641 atccaaatct tttccaatag attcttctaa ttgcatagga tttgaagacc aatttagaat
    32701 ctgatcaagc ttatcagcaa taagatcact ttttgtaagc agatttacag ttggtaatcc
    32761 aagccttaat ttgatcgacg cagcaagtaa tgcaattgaa ataaaattac ttggagaagt
    32821 gaccatagta ccatcatgca aaaacagatt tagtttttca tctgcattga tattatgtac
    32881 aaagaatggt ccactagttc tatacgcgaa taattcaatt tgtcctggtg tatcaattat
    32941 cagatagtct ggattcactt tattcacttg attttctatc tgctctattt ttgatgcaat
    33001 aagatcactc gccataatca tggaaccgtt tggacccaaa tcatattgtt tcattattgc
    33061 tagaatgtca acataatctc ttacatcaac atctggtttg tatggaagtg attcaacacc
    33121 tggatctaaa tttagaattg acgcaaagac accattttgt tcataatact gtaaaattct
    33181 tgaggctagt attgacttgc ccgctcccgc tgtgcctgtt ataaaaatcg ctttcaatct
    33241 tctgtcagaa tatatcttct tattggctta tatttgaatt cataaaaatt cagcaatgaa
    33301 ctggagaatt gcagttattc ctgtaactat gattcctatt attataatca tcattcaatt
    33361 tgacataaaa ttagatgacg tgttcgctat aggcatatta ccatttatcg ctgccatctt
    33421 agccatgatg gtaaaattag gattacaagg tctgaaacta agttacattg caagatcatt
    33481 tctaggcaat tttgattcta tcaagagatt ggcagcgatg agaataggta gtgaattcat
    33541 caaatttaca acaccaatgt ttgtcggcgc tgaatttgtg gtaatttatt atatgaccaa
    33601 gaagggtatt tcaccatcaa aggcatcgtg ggttgcactt cttgatattg taaccgaagt
    33661 ttttgctggc ggacttttat caattcttgc tggaattttt gcattactga atggtgctta
    33721 tgttgttgct gcaataattt taggaactag catatttgtc actacgttat gggttgtttt
    33781 atttttcgct tcagcaaaac atacttttca tttaccaaaa ggaatagcaa tgttacttac
    33841 aaaatttggt ggagtgcgtg gtgaaaagta tgcaaaacaa actgatgaat ggattggtga
    33901 agtctgtaca atgagtagag aaaatcttgg ttcaaagaaa tcaaagaaag tattttgggt
    33961 tggctttttt atgtctttag tctcatggac attttatggt atttcatttt tggcaatagc
    34021 cattggcgtt ggtatgacta tagaactatt cgagtcagta atggctgtaa tgggtgcaaa
    34081 tgcaatagga aatctaccaa ttacaatcgg cggttctgga ttagcggaat ttggtattgt
    34141 agcttatctt aacaatctaa acccatttaa tttggtaatt ccagaagagg gacttgaatg
    34201 gaatgctgta ataggttgga gaattgcaac atattacatt ccaatcgcag tgacttggat
    34261 tcttttaatg aaacttgctt taggtaaatt tgtaaaacct aaaacacttc atcaaaaatg
    34321 tccaatttgt ggaaatatga ttaatgatat tgaatttgcc agtcatatgc aacagaaaca
    34381 ttcaaccgca tagaaaccac tttatttttc tgttatcact ctaaatcatg aatttcaaga
    34441 ataaagtaat tgtaattact ggcgcttcaa gcggaattgg tgaggcatca gctatcaaat
    34501 ttgctaaaaa aaatgctaaa attgttttag tagctagaag aaaggaaaaa ttacttcaac
    34561 tccaaaaaaa actcgtaaaa tttacagatt caacactagt ttgtcaatgt gacgtctcca
    34621 ataaaacaca agtgaaggaa atgacagata ttgtattaga tacatttggc cgaattgatg
    34681 ttttgataaa taatgcagga tttgttattt atggaaaagt gtcagatcta agcatcgaag
    34741 agatagaatc acagatggaa acaaactatt ttggtatgat atactgtaca aagaattttc
    34801 ttccgcatat gattgaacag ggaaaaggtc atattgtgaa cgttgcatca gtgggtgcta
    34861 gttttggggt tcctggtgtt gcctcgtatt gtgcaactaa atttgctatg ctcggttttt
    34921 cagaagggtt aaaacatgaa ttatctggaa caggtgtaga tcttactgtt gtaagcccaa
    34981 taatggtaga tactccttta tttgatcatc catcttttga aaatttttca aaacgttcta
    35041 cattggttac cttaagcccc aacactgttg caaatgcaat tcttaaagct gcaaattctt
    35101 caagattaga gattgttgtt ccttcagttg caagagttgg aatttgggca aaacaaaatt
    35161 ttccatatat catcaatcca ataattggca gttcatttag aaaacagcta gaaaaaagaa
    35221 ataaatcaaa ataactccat taacaaatag aattatcgtg aaccgtcatc cactattgtg
    35281 atttcagtta attcaaattg atagattgca tccatctcaa gttccttctc ggtttttaaa
    35341 tattcaataa cttttttgtc taaaggagct ttgagtgaat caaacttgaa ttcataaaca
    35401 acgccttttt caagtttatc catctttatc ttttttgaca gatcaagtgt aactatggat
    35461 cgtccatctt caactgggtc ataaaattgg gcatcaattt tgttgtcttt atcataaact
    35521 tccagaacat atccatttct tgttaattgt tctggttttc caaacttagc attttttatt
    35581 tcatctgtaa cccaccctgg gatatcatct ttcttttttg ccattgtcta tattttctat
    35641 ttatctttct tacttttttg ccaccattcc aagtaatcat attgtttatc ttcctttgtt
    35701 gattctcgtt ggtttagctt atcacgtata tcgaaaattt gttctcttgt tgcgtacaat
    35761 ccacatctct tacaaatgaa atgttttgtt gaaggatcaa gtttcatagg atattcaaga
    35821 tctgagccta tgtccctatc atgaggcata acatactcgg catcctcctc acgtgccttt
    35881 tcagcctcag cctcaaactg catttttagc ttttttctct ctcggaaagt acattcaggg
    35941 caattaggca atgatgtcca acaaaatttt ttccataaaa gcgttcgcat aatacgattg
    36001 atcgcaaaaa atgtctggta cgtggattac atcttcatcc cattactggt cagttcgccc
    36061 atcgaacata ccccgtacca gacacaaaaa ctcatcgcac aaaactatta attctttttt
    36121 tcaagaattt ctgcaataat tttcgaggtt ttctcagcgc cattattatc aaagttttca
    36181 gaaaactctt tcaatctttc ttcatatttg ttataatcat gctttatctt ttttaccgcc
    36241 ttgatgactt gctttttgtc ttttgctaac aaacccaatt ttttttcctc tgcccattga
    36301 atttgatttg tatgttcatc ataaacgggt ataccaataa ttggttttga attggtaccc
    36361 attatttctc ccattaccgt atgtgatcca ttaatcacac aaaatttaca attattgatt
    36421 actgtttgtt tctcgttttc tgtaagaaaa ccaatatcaa tttgaatcca atcaattttc
    36481 ttttcatatg catcaagtaa cgagtatttt tttccatctt ttccaattac ttgatcaatg
    36541 gatttatcgt ttttagcatg tgaaattatt cgacgttcat ttttcatttc agaatcatgg
    36601 aaaacttctt catatttttg accggttcca tcatttgttg atttatttcc agttctcatc
    36661 caatatccaa aatcagtttc tttcaccata ttttccaaat ttgttgaggg ttcagaacta
    36721 actgatgcct tgcctgaaaa atgaccaaca tatgtaacct tattttttat actttctggg
    36781 aaattcaaat tatattcaca aattgtatat ggtggaggtg aatcggctac taatatcatt
    36841 gatgcttttt caatttgttt tgcaacaaag taaagtgctg gataaaaata actgcgagat
    36901 ttccatagtt ttggcatgta ttggtttgta acaaacagac ttggaatacc acgatttttt
    36961 gccagtacgt tagaacccat atctccatca tttatgacaa catcaaactt ttctttgtca
    37021 taaatctgtg cttctttttt tagataatgt ttgatctgtt taactaatgc cttttctttt
    37081 gctactggaa atagtatatt gagtaaggat aatggtacac taggaccatt ttttccatct
    37141 atgggtgttg gcattaaaat ttcatgtatg ttatcttttt tatctgggaa tttttgaagc
    37201 agtttttcat atatttcacc cttactgaaa taaaacatct caggtttttc agtcaagtaa
    37261 tttggtaaaa ctgcattgag cctcatcatt cttgagtaat gtccatttcc ccacggatag
    37321 atgaattgac ctattttttt cataatcttc actcttcggc tgatactgaa tctaaaatat
    37381 catgtatatt attcacaccc aatttaacat gaacaatttt ttttgttttc atagaacgtt
    37441 caacgagatt ttcaatcttc tttttaggtg atgctgaact gatgaacttt gtttttccta
    37501 tcttctcaat tttatttaga tatttttcta atccaatttc ttttgcatca tctataatat
    37561 cttcatctaa tgtggttaat ggagtatatg aaatcaaacc tttactatga atttgcttaa
    37621 caattttttc aaaaaaagat agtggatgaa ttattggtga aatactaaga gaaattcttt
    37681 taatattaac agatatggct acttttacaa gtattccagc aataacttca gccaaaacca
    37741 ttggtggtaa tttaacacct gcatttattt tgtaaaattc taaactaact tgtgtttgaa
    37801 cagttttatg aattatcaca ttaatcattt ttactaaatc caacaattca gattttttat
    37861 tataacaaac tattatcttt acatcgaatc cttgtttgat agtttcaata catgatattg
    37921 cagacaattc atcaaaaaca cagcacacca tttttttgtt ttgagaatta ttgggaattc
    37981 cttctaaccc tttatcataa taaatacaga tgtaagcaga tgattttgtc aagtgagcaa
    38041 aaagacgtct gtcatatttt tcagctgtac caggtttaat tccttttttt acagtttttt
    38101 caattaatga tgatgtagct gcaacctcaa catctcttgt aacaaatcct tttgaatatc
    38161 cttccacctg caaaaagaat ctttcatttt ttaaaaaaag atcagtagct atttttgtaa
    38221 tattttctac aattatttta aaattattct ttacttgttt tgcaattgca atatttttta
    38281 ttccaaataa atcatttata gtagatgata caaaaactgg gtcgtcagtt tcgactatta
    38341 tcactttatc atcttgtctt attttttgga atttttgatt ttggattttt agaatatgtt
    38401 taatatttgt aataaggggt ccaatcttat tttgggaaaa gattgaaggg aatactacaa
    38461 caactatgtt attttccaat atcgatatca tatggggtca taattttaga attgttattg
    38521 attttgccta gtaatgaata taaaacgaaa taaaaaatta ttcataatgg cacaatttac
    38581 ggctgaacag cttgatgagc taaatgataa attaaaaact cctgaggaag ttttgacttg
    38641 gggtttggaa aacattcatc caaaacttgc acttgcatca agttttggtg ctgaagatgt
    38701 ttgtgttatt catatgctag caaagataaa tccagaggct agagtatttt cattagacac
    38761 gggaagaata aatcaagaaa catatgatgt tatggatgaa ataagaaaaa aatacaacac
    38821 taaaattgag attacatttc ctgatgcaac tgaagtgatt gagatggtcc aaactcacgg
    38881 catgaactta ttctatgaga gtgctgaaaa cagaaagctg tgttgcggtg ttagaaaggt
    38941 acatccttta aacaaaatgt tatctacatt agacggttgg attacaggtc ttagaagcga
    39001 tcaaactcaa aacagacaat catctacaaa aattgaaatt gatgaacaac atgatggtat
    39061 gataaaactt aatccgattc ttgattggag ttgggatcaa acaatgaagt atatcaaaga
    39121 taatgacatt ccttacaata aattaattga ccaaggcttt cctagcattg gatgtgaacc
    39181 atgtacaaga ccaataaaac caggcgaaga tttacgagct ggccgttggt ggtgggagaa
    39241 tcaatctgac aaagagtgtg gtcttcacat ggatcataac aagtaaataa gaaacaaatt
    39301 ttttttaaaa acatgattag tgcacacggt ggaaaactgg taaataaagt aacaaatact
    39361 gattcatcgg gactattttc aattgacatc tcagcggatt tagcaaatga tgtagaaaat
    39421 atcgcggatg gaattttttc accgcttgaa ggttttttaa accaacaaaa ttttgaatct
    39481 gtcatttcta aaggaagact tgtaaataat actgcatgga caattccaat tgttttagat
    39541 gcagatgatg agactagtaa aaaaataaaa gaagctggag atgttttgct caaaaatcca
    39601 gaaggtactg gaattgcaat tttacatgta gaagatattt atgcatttga taaaaaaaat
    39661 actgctaatg gtgtttatgg aacaaatgat gaatcacatc ccggtgttgc aaaaacctat
    39721 ggaatgaaag atttcttagt tggcggagaa attgattata tacaaaaacc aaatgcatct
    39781 gaaatcagaa aatataggat gactcctgta gagacaagag aggcattcgc aaaggcagga
    39841 tggggaaaaa tcgtcgcatt tcagactaga aacccaccac acgttgcgca tgaaattctt
    39901 caaaaaacat ctattaccac aagagatggt gtttttgtaa acccacttat cggaaagaaa
    39961 aaatctggtg acttcaaaga tgaggtaatt ataaaagcat acgaggctat gattgaaaat
    40021 tattatcccg aaaacagatg tagattggca acattacaca cagaaatgaa atatgctgga
    40081 ccaaaagagg caattcatca tgctataatg agacaaaatt atggttgtac tcacatcatt
    40141 attggacgtg accatgctgg tgttggtaat ttctatgatc cttttgccgc tcagaagata
    40201 tttgaggaat atccagaact tgatatttca ccaatatttt tcccagcatt cttttactgt
    40261 aggaaatgtc ttacctttac taatccaaaa gtttgtccac acgatgctga atcaagagag
    40321 caaatcagtg gaacaaaact tcgttcgatg attcaagagg gaaaggcacc atcagaattt
    40381 attttgcgtc cagaagtagc aaaggtaatt attgattttg atcagccatt tgttgattag
    40441 atttttatct gtacaaacat cgtgctagat tatgaaatat attttatttt tactggttct
    40501 tctaatctgt acaacatctc cagtttttgc ccaagagccc aagataggag attttgctga
    40561 tcctgttaac ccatctctgt ttacggagaa aattgaaatc ccttatcatg ttttcaacac
    40621 aatttcaaga gatgcagaaa taactgaatt agaaaaccaa attacgggaa gttggcagtt
    40681 atcacttcaa aatgatttgt tgtatggaaa tccaaatggt aatgccgtta tccgtatcta
    40741 tgatgtagac atagctgaca aattccttga gattgggatg ggcagttatc ctgactacaa
    40801 atattggata tctgcacaag taccagaaac aggttatgtt atcatatatt ctaacaatga
    40861 caatggttgg ataaaaggaa cagaaacaag tatcacatat tctgagcaaa atggactttc
    40921 agtgaataat ggacaaagga ctgtattatc aaatcttgat gtttcaccat ttgcaattaa
    40981 agcgtattct gcctatggaa tggaaagctc tagtgatcct ccagcaacaa catcaggcac
    41041 atttatcata aaaattattt cagctgatta tggagaaaat ccatattcta tatttccatt
    41101 tgttttaact ggtattattg gactcgtaat tggtatactt gttcttacta agaaacgctc
    41161 ttagtttgaa aataattaca tgtttttcta attttgcata cgtcacacat tggagaaatt
    41221 ggtttacaga tgttttgacc gtacatgaca aaagtattgt taatatcaag ccaatacttc
    41281 ttaggaatct tttctcttag agccatctct gtttcttcgg gcatttttgt atctaccaaa
    41341 cccaacctat tagagattct atggacatga gtatccacag gaattgctgg tttttcaaat
    41401 gcatatacca aaacacagtt tgcagtcttt cttccaacac ccggcatatc aacaagcttg
    41461 tctatatcag caggtacttt tccattatat ttggagagga gtaagtttgc aacctcgatt
    41521 attcgttttg attttacatg ataaaatcca actgacttga taactttctc aacatccctt
    41581 accttggctt ttgccagttt ttgtggagta ccatataatt tgaataactt gtcagcagcc
    41641 ttagtcgtat tttcgtcctt tgttctggca gaaagtattg ttgcaatcaa aattctaaat
    41701 ggtctagcag tttgaacttt atgtaatcct tctaacgctg tatatctagg tggatttaca
    41761 tcaaccatag tttttttcat gccgtcgaga atttttttca tatcatatca aaaattcagt
    41821 ttactcatct agatcttcta gcgtctttct ttgtctggta ctaagataaa atgcatgtgc
    41881 actaataaca aaccctgcta accaaatttt tgtagcgaaa attaaattcc atggtcgtag
    41941 aggatctggg tattccacca taagcaaatc aatatctggt atagtgttct gtaaaatcat
    42001 ttgtgatgtg atttcagcat tcaatacagt aaaactccat aatacttcat agagacaaat
    42061 aatagcaaat ccaagcaaca ttaactgggt caatgccatt ctatacgaat tcaagccgac
    42121 tgtttttttc catcctattc tggaacaaca ataccaacca attattgtag aaaagaagag
    42181 ccaagtagaa agatttgcaa aattctcaaa cggaacttgt gtttgtacta ggacttcacc
    42241 cataacataa attccatttt cattccattc ttcactcata gcataaattc catagaatgt
    42301 tatggaaccc atgataatca tacatagata aaaaatatag agaaaaatct tatactgtct
    42361 atcactttca tgtagatgcc gtctcttcat tattgcgcta gtaattgttt atataaaaac
    42421 catataattt gttaacgaaa tttattaaca gtaaatatca ggaatgctag tgaatgtccc
    42481 aataaatatt cctgacattg atgaacagga aatcagagag gtcacctctg ttttatcaga
    42541 aaaagcatta acctcagctt catttgaagg cggaaaacgt gttagacaat ttgaagatct
    42601 tttatcaaat tttgttaaat caaaatttgc tattgcagtc aattctggaa catcagcact
    42661 gcaggcatca ttgtatgcat tagatatcaa gccaaatgac gaagtattga taccatcatt
    42721 tacatttgtt gcaactgcaa actctgtaaa atcgattggt gccaaaccag tatttgttga
    42781 tattttgaag gataatttta cgatggatcc agacgatatt aaaaagaaaa taacaaaacg
    42841 gacaaaagca ataatcccag ttcatctgta tggccacgta gcatatatgg aagaaattat
    42901 ggaaatagca aaaaaaaatc atcttgaaat tattgaagat gcttcacaat ccctaggctc
    42961 aaaattcaaa ggaaaacatt ctggaacttt ttctcatctt ggatgtttta gtatgtatgc
    43021 agctaaagta atgactgctg gtgaaggagg tgcaattgta acagatgata aaaaattatt
    43081 tgaaaaactt aaacaaatcc gtaaccatgg attatcaaaa catcatatta caagtaagtt
    43141 tggtctaaat ctacgactac ctgaaattaa tgctgctata gcaaaaatac aaatgaaaaa
    43201 attaccaact ttcttaagac aacgaaaaaa gaacgcacag atactaactg aacttttgca
    43261 aaaccatgac ataattttgc cgaaggaacg aaaaaatgag gtaatgaatt ggtatctgta
    43321 taccgttgca ctaaaacaac gagataagat aatgaataag ctaaattctg ctggaattgg
    43381 cgcagcagtt tactataacc catcaataca tcagacacca tattataaaa ccaaaaaaac
    43441 tctaaccact acagaatggg ctagccatca tgtgatcagc ttacctatac atcccaaggt
    43501 gaaaaaatca gatttattaa aaataaaaaa aatcctaagc gcttatagaa attgaattaa
    43561 taaccatctt tttggcgttg tttattaatt tcattttttc tttgatattc tttcaaaata
    43621 tcatcaggag ttagaccaag ttccatagat gcctgaatta agaaatgcca tatatctatc
    43681 aattcttctc gtgcttctga ctgattgaaa gatgtaggtt ttttccacca tttccaattt
    43741 gttgtacgct gaagctcaac tacttcatgc atcatagctg tacataatgc agaaagtttg
    43801 gcttctgtat ctttgggata tcgatccaaa tccatcattc tttccaaact tttttgaaga
    43861 gtaaaaatct catccaattt atcagccata gtatctacaa aaaatgaaca cgtataactt
    43921 ttcagaaact aaccattttc tcatactgct ttactcgttt tgtaatatct gcctttgtta
    43981 actccaataa tcttcccaac ccatattttt gaacagcaaa tgaacccata acattgccgt
    44041 acataataga ttttttgata gttgataggt tagttttttt cttggatgca agataaccta
    44101 tcatcgcacc agcaaatgaa tctccagcac cagttggatc tacaatattt cctaatggga
    44161 acccaggaga tggaaatata gcatcttcat agaataaaag tgaaccatgc tcggcttttt
    44221 ttataataac atattttgtt ccccaaccca ttatcttttt tgcacattta atcaaattga
    44281 attccttagt tagtaatttt gcctcttcat cattaatcac caccgcatct acctctttga
    44341 acatccttac tactgaagct cttttggtgt taatccaaaa atctattgta tcacacattg
    44401 aaaattttac atcatcaaat tctttaatta aagagatatt ttgttcagga tcgttattag
    44461 caaggtatac aaatttcgat tttttatatt cttcaggaac tacaggcttg aagtttttaa
    44521 gaacatttag atctgtacgt agtgttgttc tggtgctaaa agttttgtta taacgaccat
    44581 catacctaaa ggttttacca ttttttatgg aaagcccttt cagatcaaaa tactgttcaa
    44641 gtattctatg atattttctt gggaaatctt ttccgattac agcaattagt ccagttttgg
    44701 taaaaaaact agatgaaatt ccagcaaaag tagctgctcc acccaaagca tcttttaaaa
    44761 tttttgtggg ggttctaatg gtatcaagtg cagttgatcc aaagactgtt aacatatcat
    44821 cgtgcctata atttcgtata aattcttttc aaatatgatc ataatagtgt gtaaaaattt
    44881 tgatactttc ttaaagctac ttagagctaa caaaataaat caaaaattag atgaaaatat
    44941 gcattggcta aaattaaaat aaaaattggg gaaaatgaaa ttgaagttga ttctcgtgat
    45001 ttctatatag ataataatac aataggagaa gtgattgaaa acatttcgat atatctacaa
    45061 ggaagtaaca agaatttttt tgagcagatc cctattcaag aaaatggaga aggaatgaat
    45121 aagctcagtt tacttgaaga tgcagaagtt catgaaccag aattcagtga tccagttttg
    45181 gttacgatag acgaactaca tggaaaactt gtaacattat cgacaaattc tttttttgat
    45241 cagcctagaa cagtatctga aactgtaagt gaattacgag agaatggatg gattgcaagc
    45301 ccattggatg tatctaaagt acttgccaaa atggcttttt gtaaggaact tctaaaaaat
    45361 aagcaggatg acagaagctt ttattttgtt aaggaagcat tattagctga ataatcttca
    45421 taccatcttc taaagtataa aataatgtga gtatagtggt atatgggtag atttattgat
    45481 ttgtgaaaaa tgtggtgaaa ctataaaaaa agatgaggaa agaacacaca aaattttttg
    45541 ttattcatgg ctcaatgaag ataaaaatca ataaaaaatt attcagatat tgacacgtgt
    45601 aacattttag cttcttccaa cggacagtca ttattgtttc taggtaaatt ttctatttta
    45661 tgagctacat ctattccttc tgttacttca ccaaatactg tatattgatt atctaaactg
    45721 gcagtgttat tatctgaagt tacaatgaaa aactgtgaac ctgcgctatc aggatccgtt
    45781 gctcttgcca tggaaacaat tccagttaaa tgtgatctag gattaaactc ggcatttatt
    45841 gcataaccag gaccacccat accccaatta ctttgattgc tttgttttgt attaggatca
    45901 ccaccctgaa tcataaatcc aggtattact cgatgaaata aagttccatc ataaaattga
    45961 gaatcagata attttctaaa atttctaact gtttccggtg ctaattctgg cagcaattta
    46021 aattttatat tgccaaaatt ggtttctatc gtagcataag ccattttctt tattcttgaa
    46081 tttgggttta agaatctttt gcagttaatc aaacatcttt taaaaatgtt ttattttgaa
    46141 atatagaaaa ctagtcttat atagaacaat aaaataatac cactgtgaat cgtacaaatc
    46201 aaagtacaat tattaaagca gaaatcgatg atttgcttac atcaggtgtt aaagacaaac
    46261 aagaaattta ttcaaaggtc gttgacaaat tgggtgtacc aagacctact gtaagacgtg
    46321 tagccggtga tcttagaaac gaattgcttg caaaaatttc aatcttacaa tctgaaaccc
    46381 agacaactga aaaaactgaa taatactcaa acttaatttt ttattttatt gtttacaaat
    46441 gtgaatttca agcattactg ttatattcta ccaaattttt gaaaatgtat gggctatcta
    46501 tataatcact tagcaactgt tgtttgtgct gtctttgcag gtgttttgac tttactttgg
    46561 cctttattcg tagattttgc tgctgtgtta gatctagtat tcgtgatggc tgtgccaatc
    46621 atgtggttcc taacagctgt ttgttttgta gcacaaaaaa gtacagatta cgtacatgga
    46681 caccagtcca gtcacaagtc caccaaaaaa ctaatttaca cttctggcgg acaagtatct
    46741 aactaaaata tcatttacag cattcacatt tgtgttcctc attatggttt tctttgcagc
    46801 caggacaact gacagcaccc cctagatgac atttacaaat acagacatta gattcactca
    46861 tacactgaca aacgtattag tcctaatagg ttttgaagtt atttttagta acaggatttg
    46921 tagtgaaaaa cgggcttgga gggcttcgat ccctcgacct gtaacttagg aggctaccac
    46981 gctatccata ttgcgcgtca tttgactctg cgccacaagc ccaaaaaaaa aacacatgta
    47041 tgataattaa gcataattag actagatagc atattttgaa atcctaacta atggtctatc
    47101 caagtcatgt ttttcacatt aaacgttaat aatccacaat aatttttgaa tgacacgggc
    47161 cgatggtcta gttggttagg atactgcctc gacacggcag tggtcgagag ttcaaatctc
    47221 tctcggccca tttattctat taatttagat catatgatca taatgatttt tcaaaattca
    47281 cttcaatcag ttgccatgca gtttcaacag tatattgacc aattacacta tatgttacat
    47341 cctctccatt tacaattgct gaatattcgt cttggttacc gtcagataga ggaatgacga
    47401 tgtaactatc taaaggaagt tctttgccag ggaaaaacgg aattcttccc gtcataggta
    47461 tatcttcaga tgaataagat gaacttccca tactttttcc attaataaat aattcataac
    47521 taactactgg tacagtaata gttttttcag catgattttt tagcaaaaat acaactttca
    47581 atttaatttt attatcaata ctatcaacat tcaccaattc aatactacca agcttcaact
    47641 cacttttttc taattcagga atatcaagca gagaaacata caccattcct cccataaatg
    47701 ctaataaaag tacgatagcg atatacagaa ttattttacc aggaggtttc aaatcatttt
    47761 tttgtgagtc cttgaattaa aaaaccttgc cagaattcat atgtctaata tttgaggagc
    47821 taattggata ttcatggcca tagaacaaac cattatcact tggatccatc ttcttagtgc
    47881 atctatttgg gtagggggtt caattttcct tggggctgtt ttagcaccac ttttgaaaaa
    47941 aatgtcgttg tctattgatg aaagattagc attaatgatc aaaactggta gacgctttaa
    48001 caagatcgct ctcccttcac tcatgatttt gattgccaca gggatttaca actctcattt
    48061 agtattgcag agtccagaaa tattattttc aagcagttat ggggcatatt taattacaaa
    48121 aatcattctt gttatagctc tgattatcac ttttgcggtt catatcagaa tatttagcaa
    48181 agatgttgaa gaaaaaataa tagctaagca aatacctgat aatgaattac aaaaactaaa
    48241 taaaaaaggc atgatcttag gagaaactac agttataatt tctgtaacca tattattttt
    48301 tgcagcgttg cttaataccg gcatttgata tattttttaa acattgtcta gaaacaatat
    48361 tatgaatgga cttttaattg gcagatttca accatttcat ttaggacatc tggaggccgt
    48421 gaattttgca ttggataagg tagaaaatct ttggattggt attggaagct cgaataaata
    48481 taatgaaaag aaaaaccctt tttctgctga tgagagaagg aaaatgatta ctttatcagt
    48541 taatgattca attatcactc gcatcaaaat ttttgatata cctgatgttg atgatcatga
    48601 gaaatggacc tacagcatag ataagattgt accagaatac aatctagttt tttcaaatga
    48661 taaatttact gataatttgt ataaaagacg taatattgat gtgataacgg ttccattaaa
    48721 agatcggcaa aaattttcag gaacaaacat acgcgagcta atagcggacg ataaaaactg
    48781 gcaagatttg gttccgcaag gaacgcgaaa agtcctagat aatctgaacg ccaaagaacg
    48841 tctaaaagat ctgtaattat aaataaagcc atataacaat tcaatcaggt aattattttg
    48901 gaatatctat caatgctacc cggtccgaca aatgtaccaa acagagttat gcgagcaatg
    48961 cttgcaccaa taattaatca tagaagtgat gattttgttg aattgtatac tgatgttatt
    49021 tcaaaaacac agcaagtctt tgaaacacag aatgatgttg tagcattatc tgcatctgga
    49081 actggggcag ttgaagcagg cgttgtaaat attgttaaaa aagatgataa agtcatcata
    49141 cctgtaaatg gagaatttag taacagatta tcacaattaa tagaaggtca aggtgcaaat
    49201 gtaatcagat tagaaacacc tcctggagaa aatgcaactt ttgatcaagt aaaagaagct
    49261 tttgataata acaacgatgt caaagcattc tatgttgttc ataatgaaac ttctaccggt
    49321 acaatggtaa attatctcga taaagtctca gacctaacat cacgtaatga ttcaatgtac
    49381 atagtagatt cagtttcatt actcggtggc acatcattac ctgttgataa atggaacatt
    49441 gatgtatgta tgactggtgc acaaaaagca attgctgctc cacccggaat ttctccaata
    49501 tctataagcc ctaaagctaa aaaatacatg attgaaaatc cacctgcaac aatgtacttt
    49561 aacctagcac gatatttcaa gtactacgaa gaatctaaac atactcc
//
LOCUS       DASCPJ010000103         5956 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001676318, whole genome shotgun sequence.
ACCESSION   DASCPJ010000103 DASCPJ010000000
VERSION     DASCPJ010000103.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001676318"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="QGF01_04940"
     CDS             complement(<1..479)
                     /locus_tag="QGF01_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HJN20265.1"
                     /translation="MNENKNQFVLSGDTISGSYEPAQNVILNDNRLLSTAIGFCEIND
                     NEANVSPLTGFYIPRSDDLVIGKIKSHTSMVWEIDINSYHVGILPAFDIFGKDYNPSK
                     HDLSSKLNIGDLVAVRIVSGDTARDPLVTISGENLGKIDSGELVKVSPAKISYLISK"
     gene            complement(476..1165)
                     /locus_tag="QGF01_04945"
     CDS             complement(476..1165)
                     /locus_tag="QGF01_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HJN20266.1"
                     /translation="MADKFSIAKYTFEGDRFEILVKPDPALDYKLGKLKDINSILVSD
                     EIYSDAGKGTRASSEKLQKAFKTEDATTIAEKIIEKGDLNLTTDQRRKMTSEKRKQII
                     TFIAKTFVDPRSHLPHPPQRIEQAMTDARVSIDPFKNVDEQTKDIIEKLRSIIPLKSE
                     NLVLEISVPAQYVAQSYTVLKSTGVLKKEDWQPNGSLKAILEIPAGLRPNVIDRLGAV
                     TKGTAFVEVLK"
     gene            1250..1852
                     /locus_tag="QGF01_04950"
     CDS             1250..1852
                     /locus_tag="QGF01_04950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HJN20267.1"
                     /translation="MSIIQTLNANEKKITLKLKGFPLQYANALRRICLNGVPIFAIDT
                     VDVIENSSVLADEGIAHRLGMIPLKTDVTRFDESDSRVMFVLDSGDIDTTKTITSADL
                     STNDEVVKPVSDKIPIIQLAPGQKVKLEAYARLGKGTEHAKWNSANVSILTDSDKKDE
                     HLLTVETTGALEPMEIVSAGIEELNRRLEEFKEDITNLDK"
     gene            1895..2242
                     /locus_tag="QGF01_04955"
     CDS             1895..2242
                     /locus_tag="QGF01_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HJN20268.1"
                     /translation="MANQTVLHMVKDLKQASKKNDAPIWSKLASIALKTSSSKRVVNL
                     TKINNITKDGDVIFVPGKILGTGNLSHKITLSSFSISVNAAKKVIQSGGNILKFNDMI
                     TKFPTGKGVMILG"
     gene            2235..2774
                     /gene="rplM"
                     /locus_tag="QGF01_04960"
     CDS             2235..2774
                     /gene="rplM"
                     /locus_tag="QGF01_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HJN20269.1"
                     /translation="MADAKPDAKAKPDAKAKPDAKAKPDAKAKNKPVDKTSRPIVVDG
                     TNHIAGRLCSNTAKLLLQGNRVSIVNSENIMISGRKKGIVGEYREFLKIASILHPKHG
                     PFHPRKPDTIISRMVRGMLPRDKPSGQAALKRLRAYVGVPKELKSIKKTQFEKSIIRK
                     SNAYYTSMGELGEYVGWNK"
     gene            2771..3217
                     /gene="rpsI"
                     /locus_tag="QGF01_04965"
     CDS             2771..3217
                     /gene="rpsI"
                     /locus_tag="QGF01_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HJN20270.1"
                     /translation="MIKNEIYFATRKTASAHVHITKGVGKVRINNVPLEMIGQETARE
                     VMLTPLEICGEENRNKINVSVRVSGGGFMGQAYAVATAMSRALVGWTKHRNDPKEHPF
                     TKSVRTDLKKKIEEFDKYLISGDARRKEPKKFGGPGARRRKQKSYR"
     gene            complement(3225..3575)
                     /locus_tag="QGF01_04970"
     CDS             complement(3225..3575)
                     /locus_tag="QGF01_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HJN20271.1"
                     /translation="MNELLQKWVNAIKSGDPKQVTALYLDNGILLGTFSNKERIGHEL
                     ILEYFENLLKQPVEVQIISENSHVFASDAVNSGHYNFVVSGKTINARFSFVYHKDNGE
                     WKIISHHSSVMPET"
     gene            complement(3610..4368)
                     /locus_tag="QGF01_04975"
     CDS             complement(3610..4368)
                     /locus_tag="QGF01_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJN20272.1"
                     /translation="MKPNVLAKKIGFYTLIIIAWQGIDSAEIWPDNIFPSPFEVLEDL
                     AYGISDASLFFGIGTSLLRLVIGLGIAIAGGLVLGIFMARVETVNQTIGSLVLGLQSI
                     PSIAWVPLAILWFGLTDSGIIFVTAIGAIFAVTINTYTGVKNINPSYIEAARNMGAKE
                     GQLIITVLIPAAFPYIISGFKQGWAFAWRGVIGAELLFSFLGLGFLLNVGRQLNDVSQ
                     VIAIMIVIMGIGILVDGVVFKRIEDKVMTRWGLR"
     gene            complement(4365..5120)
                     /locus_tag="QGF01_04980"
     CDS             complement(4365..5120)
                     /locus_tag="QGF01_04980"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJN20273.1"
                     /translation="MGKLEAKNIEKYFKHDGKQLKTLDGINLNVNDGEFVCIVGTSGC
                     GKSTFLRTIAGLETPDSGKILLDGQPLSDTGPDRIMVFQEGALFPWLNVRDNVEYGLK
                     VAGIPKEKRNEISHRYLDMMQLTQFATSYTYQLSTGMKQRVAIARALVMEPDVLLMDE
                     PFAALDPQTRDLLLVEMQLIWQKTKKTIIFVTHNVAEAVVLGTRVLVFSHRPAKIKKE
                     VEIDYQRPRLTDDENLLPYQKKILAELRPEVKK"
     gene            complement(5123..5947)
                     /locus_tag="QGF01_04985"
     CDS             complement(5123..5947)
                     /locus_tag="QGF01_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJN20274.1"
                     /translation="METKLFDSGPQVIESIFSGSIDVAYVGPGPIINGFLKSDGKDIK
                     ILSGAASGGASFIIQPNSGLESLENFDGKRIASPQISNSQDVSLRYYLESHGLKSVEK
                     GGTVFVLNISNPDIYTLFAKGDIDGAWVPEPWATILVQELDGVRLFNEEKLWPNEEFA
                     SVLLIVRTKYLENNPETVQKWVESHEKTVTWINSNPNKSKSLFSNFLMDYMGKSLPTK
                     IIDESFSNITITSDPIKNSVIIFAERADSLGYLGRDGYNLDGIFYNAVLNVNNGDL"
BASE COUNT         1993 a         1062 c          999 g         1902 t
ORIGIN      
        1 tgtttagata taagataaga aatttttgca ggagagacct ttactaattc acctgaatca
       61 atctttccta aattttcacc agatatagta accaaaggat cacgtgcagt gtctccagat
      121 acaatcctaa ctgcaacaag atcacctata ttcaattttg aggaaagatc atgtttacta
      181 ggattatagt cttttccaaa tatatcaaat gccggaagga ttcctacatg gtaagagttt
      241 atgtcaattt cccataccat agaagtatgt gatttaattt taccaattac taaatcatca
      301 cttcttggaa tataaaatcc agttaaagga gatacattag cttcattatc atttatctca
      361 caaaatccaa ttgcggttga aagtaaccta ttatcattaa ggataacatt ttgtgctggt
      421 tcatatgaac cggaaattgt gtctcctgat aaaacaaatt gatttttatt ttcattcatt
      481 ttaaaacctc tacaaaagca gtacctttag ttactgcacc taatctatca atcacatttg
      541 gtcttaatcc agctggtatt tctagtatcg cttttaatga accgtttggt tgccagtctt
      601 cttttttcaa gacacctgta gattttaaga cagtgtatga ttgcgcaaca tattgagctg
      661 gtacagatat ttctaataca agattttccg attttaatgg aattatcgaa cgtagtttct
      721 cgattatatc tttagtttgc tcatctacat ttttgaatgg atcaatactg acacgggcat
      781 cagtcatagc ttgctcaatt cgttgtggtg ggtgaggtaa atgagaacgt ggatcaacaa
      841 aagtttttgc gatgaatgtg attatctgct ttcttttttc tgaagtcatt tttctacgct
      901 gatcggttgt taagtttaga tctccctttt ctatgatttt ctcagcgatg gtggtggcat
      961 cctctgtctt gaacgctttt tgtagttttt ctgatgatgc tctagtgcct ttccccgcat
     1021 ctgaataaat ttcatcagag actaaaatgg agttgatatc cttcaatttc cctaatttgt
     1081 aatctaatgc aggatcaggt ttgaccaaaa tttcaaatct atcgccctca aaggtgtatt
     1141 tagctataga gaatttgtct gccatggcaa taataggagg tattattgat attaatctat
     1201 aattatcgag ctagattttt atttggaagt taaagttttt cagtaagatt tgtctattat
     1261 tcaaacgcta aacgccaatg agaaaaaaat tactctcaaa ttgaagggtt tccccttaca
     1321 gtatgcaaat gcacttagga gaatatgcct taatggtgtt ccaatttttg ctattgatac
     1381 tgtggatgta attgagaatt catctgtttt ggcagatgag ggtatagcac atagattagg
     1441 tatgatccct cttaaaacag atgttacgag gtttgatgaa tcagattccc gagtaatgtt
     1501 tgttcttgat tctggagata ttgatacaac caaaactata acttctgcag atctttcaac
     1561 gaatgatgaa gtggtaaaac cagtatctga taaaattcca attatacaat tagcgcctgg
     1621 acaaaaagta aagcttgaag catacgcaag acttggtaaa ggaacagaac atgctaaatg
     1681 gaattctgct aacgtttcaa tattaactga tagtgataaa aaagatgaac atcttttaac
     1741 agtagaaacc actggagcgc ttgaaccgat ggaaatagta tctgctggta ttgaagaact
     1801 caatagaaga ttagaagaat ttaaagaaga tattactaat ttggataaat gatgttcata
     1861 ctgttatatt tgggttataa gtggtgatga tttaatggca aatcaaacag ttttgcatat
     1921 ggtaaaagat cttaaacaag cttcaaaaaa gaatgatgca cctatttggt ctaaattagc
     1981 aagtattgct ttaaaaactt catcttcaaa acgagtggta aacctgacaa aaataaataa
     2041 cattacaaaa gatggcgatg ttatttttgt accaggtaaa atattaggaa caggaaattt
     2101 atctcataaa attacattgt cttcattctc tatatctgta aatgctgcaa aaaaagtaat
     2161 tcagtctggt ggcaacatac taaaatttaa tgacatgatt acaaaattcc caactggaaa
     2221 aggtgtaatg attcttggct gatgcaaaac cagatgctaa agcaaaacca gatgctaaag
     2281 caaaaccaga tgctaaagca aaaccagatg ctaaagcaaa aaacaaacct gttgataaaa
     2341 ctagtagacc tattgtagta gatgggacaa atcatattgc aggaagactt tgttctaata
     2401 ctgcaaaatt attacttcaa ggcaatagag tatcaatagt caactctgaa aatataatga
     2461 ttagtggtcg aaagaaaggc attgttggtg agtatagaga atttctaaaa attgcaagta
     2521 tactacatcc taaacatgga cctttccatc ctagaaaacc tgatactata attagtagaa
     2581 tggtaagagg aatgcttccc agagataagc catctggtca ggctgcactc aaaaggctaa
     2641 gagcatatgt gggcgttcca aaggaattga aatctataaa aaaaactcag tttgaaaaat
     2701 ctataatcag aaaatcaaat gcttattaca cgagtatggg agaattagga gaatatgttg
     2761 ggtggaataa atgataaaaa atgagattta ttttgcaaca agaaagactg ctagtgctca
     2821 tgtgcacatt acaaagggtg taggtaaagt tagaataaac aacgtgccac ttgaaatgat
     2881 aggacaagaa actgcccgcg aagtgatgct gacaccttta gaaatatgtg gagaagaaaa
     2941 tagaaataaa attaatgttt cagtacgcgt tagtggtggg ggttttatgg gacaagccta
     3001 tgcagttgct actgccatgt ctagagcatt agtaggatgg actaaacata gaaatgatcc
     3061 aaaagagcat ccattcacaa aatcagttag aactgaccta aaaaagaaaa ttgaagagtt
     3121 tgacaagtac ctaatcagcg gagatgcaag aagaaaggaa ccaaagaagt ttggtggccc
     3181 tggtgcaaga agacgaaagc aaaaatccta ccgatagaaa atactcaggt ttcgggcatt
     3241 actgaagagt gatgtgagat aattttccat tcaccattat ccttgtgata tacaaatgaa
     3301 aaccgtgcat tgattgtctt accacttacc acaaagttgt aatgaccaga atttacagcg
     3361 tcagaggcaa aaacgtgaga attctctgat atgatctgaa cttcaacagg ttgttttaac
     3421 aagttttcaa aatattcaag gattaattca tgtccaattc tttccttatt ggaaaaagtt
     3481 ccgagcagaa ttccattgtc tagataaaga gcggtgactt gttttggatc accacttttt
     3541 attgcattga cccatttttg taaaagttca ttcattacaa gatatagaat tttttaattg
     3601 taatttaatc tagcgcaaac cccatctggt cataacttta tcttcaattc tcttaaagac
     3661 cactccatca accagtatac caattcccat gattactatc attattgcta ttacttgaga
     3721 tacatcatta agctgacgac caacgttaag aagaaatcca agacctaaaa atgaaaataa
     3781 taattcagcg cctataactc ctctccatgc aaatgcccaa ccctgtttga atccagatat
     3841 gatatatgga aatgcagcag gaatcaatac ggtaatgata agctgaccct cttttgcacc
     3901 catatttctt gccgcttcaa tgtaggatgg attgatattt ttaacaccag tgtaagtgtt
     3961 gatggttaca gcaaatattg caccaattgc cgtaacaaaa ataataccag aatctgttaa
     4021 tccaaaccaa agaatagcaa gtggtaccca agcaatagat ggtatcgatt gtaatccaag
     4081 aactaatgaa ccgatagtct ggttaacagt ttctaccctc gccataaaaa ttccgagtac
     4141 caaaccacca gcaatagcta ttcccaaacc aataacaagt ctcaataaac ttgttccaat
     4201 tccaaagaat agacttgcat cagatatacc atatgccaag tcttccagaa cttcaaatgg
     4261 agatggaaat atgttatccg gccagatctc ggcactgtca ataccttgcc atgctatgat
     4321 tatgagtgta tagaaaccaa ttttttttgc tagcacattg ggtttcattt ttttacctca
     4381 ggtcgaagct ctgccaaaat ctttttttgg tatggtaaaa gattttcatc atctgttaat
     4441 ctaggacgtt gataatcaat ttctacttcc ttcttaattt ttgctggtct atgagaaaat
     4501 actaaaacac gcgttcctaa aacaactgct tccgcaacat tatgtgttac aaaaataata
     4561 gttttctttg tcttttgcca tattaactgc atctccacta acaaaagatc tcttgtctgt
     4621 ggatccagtg ctgcaaatgg ctcatccata agtaacacat caggttccat taccaatgct
     4681 ctagctatag ctacacgttg tttcatccca gttgaaagtt gataagtata tgatgtagca
     4741 aattgagtaa gttgcatcat atccaaatat cgatgtgata tttcgtttct tttttcttta
     4801 ggaattccag ccacttttaa tccgtattct acattatctc gtacatttag ccatggaaat
     4861 aatgctcctt cctgaaatac cattatccta tccggaccag tatcacttag tggctgacca
     4921 tcaaggagaa tcttaccgct atctggtgtt tccaatcctg caatagtgcg aagaaaagtt
     4981 gatttcccac atcctgatgt gccaacaata catacaaact ctccatcgtt aacatttaga
     5041 tttattccat ctaatgtctt gagttgtttt ccatcatgct taaaatattt ttcaatattc
     5101 ttggcttcta atttacccat ttctacaagt caccattgtt cacatttaaa acagcattgt
     5161 aaaaaattcc atccaaattg taaccatctc ttccaagata tcccaaagaa tcagcacgtt
     5221 ccgcaaatat tattacagaa tttttaattg gatccgacgt gattgttata tttgaaaaag
     5281 attcatcgat aatttttgtt ggtaatgatt tacccatata atccatcaaa aaattactga
     5341 agagtgattt tgatttattt gggtttgaat ttatccatgt cacagttttt tcatgtgatt
     5401 ccacccactt ttgaacagtt tcagggttat tttcaaggta ttttgttcta actatcaata
     5461 acacggaagc aaattcttca ttgggccata atttttcttc gttaaataat ctaaccccat
     5521 ctaattcttg taccaatatt gtagcccatg gttccggaac ccaagctcca tcaatatcac
     5581 cttttgcaaa caaagtatag atatcaggat ttgaaatatt caaaacaaaa actgttcctc
     5641 ccttttcaac tgatttgaga ccatgtgact ctagataata ccgaagagat acatcctgac
     5701 tattacttat ttgtggactt gcgatgcgtt ttccatcaaa attttcaagt gattctaatc
     5761 cagaatttgg ttgtatgata aaacttgctc caccactggc agcacctgat aaaattttaa
     5821 tgtctttacc atcagatttt aaaaagccat taatgattgg acctggtcca acataagcaa
     5881 catcgattga acctgaaaag attgactcta ttacttgtgg accactatca aataattttg
     5941 tttcaatttg tttctc
//
LOCUS       DASCPJ010000104        14101 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001682414, whole genome shotgun sequence.
ACCESSION   DASCPJ010000104 DASCPJ010000000
VERSION     DASCPJ010000104.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 14101)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14101)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14101
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001682414"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="QGF01_04990"
     CDS             complement(<1..246)
                     /locus_tag="QGF01_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20275.1"
                     /translation="MEIKNIIEKLLTKEFPIGVGGCRNHDFRYDCCEYDITVFDEQWE
                     LQTLLSTIKEKKKQLFNADVKNCLVESQVCITKATNGL"
     gene            433..508
                     /locus_tag="QGF01_04995"
     tRNA            433..508
                     /locus_tag="QGF01_04995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Glu,seq:ctc)
     gene            558..1217
                     /locus_tag="QGF01_05000"
     CDS             558..1217
                     /locus_tag="QGF01_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase"
                     /protein_id="HJN20276.1"
                     /translation="MTLRDLSLKREYRSDKDDLLAEFFVPCLTNCIQYDRAIEFISIK
                     SLSTLSFGLDSITKKHAKIRLISGHRFSTSDLNMLKKIYQNGYGHKFNGNMIRDSKIS
                     HLQKVIKDFKLEIKIAIPNSELVDGSFEERLGIFRDENDDVVAFTGTSNVTFDAQNRN
                     FESIDVFTSWDDKIRTDAKIENFEKLWNNETHYVEVYDLIFAEKNNLLKYTTEWAVDK
                     Y"
     gene            complement(1218..1790)
                     /gene="endA"
                     /locus_tag="QGF01_05005"
     CDS             complement(1218..1790)
                     /gene="endA"
                     /locus_tag="QGF01_05005"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HJN20277.1"
                     /translation="MAFESDSKIQGVFIKNQTLILDPDIQHDLNLKGYGEMKENKLIL
                     KPFESLYLLHVGKLVIFKGKKNLNFDFLLEIYRQLDENILTNFLIYRDLRTRGYTVKD
                     GFGFGSDFRVYNKGDFGEKNAKFLVFGLNEGKQEKVGKLQKKIQEITSMGKEPIIAVI
                     ERRGEIIYYKISRIKFQQNIEKINMDDFDF"
     gene            1870..2361
                     /locus_tag="QGF01_05010"
     CDS             1870..2361
                     /locus_tag="QGF01_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HJN20278.1"
                     /translation="MHGECYRRGNGQPYTRKEYIKGKPQIKIAKFHGGQKGKYDFSVQ
                     LMLNEKLQIRHMAIESTRLAANKTLEKTVGETGYWSRLRIYPHVLLRENKMIAAAGAD
                     RLQEGMRRAFGKAVSLGARVKAGQIIMEMHVKKEHVDAATKALKSACVKLPGTAKINV
                     VKL"
     gene            complement(2364..2633)
                     /locus_tag="QGF01_05015"
     CDS             complement(2364..2633)
                     /locus_tag="QGF01_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20279.1"
                     /translation="MDEKVQKVLEEKILEIFDKKNEINSITNSSELLSSDKNSFRYGI
                     IFGRLYNSFYYQYRRIVKRDPTEEEFSEFIQILKQHEKEFLGLSF"
     gene            2716..2789
                     /locus_tag="QGF01_05020"
     tRNA            2716..2789
                     /locus_tag="QGF01_05020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2751..2753,aa:Gly,seq:gcc)
     gene            complement(2795..3241)
                     /locus_tag="QGF01_05025"
     CDS             complement(2795..3241)
                     /locus_tag="QGF01_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20280.1"
                     /translation="MANKELEQMLERVHKDIDIFDFDGKNVPRIILSDRQFDNMMLKI
                     QGKPISVDTNLNILQDGLGHVFVEITLDFSHGDIHNKFLIYANDTLNFFEALADTTMF
                     ALSPPDHSQVNQDKVFMVQLPKPERAVNALDIIKTGLQTNHQNLKS"
     gene            complement(3245..4567)
                     /locus_tag="QGF01_05030"
     CDS             complement(3245..4567)
                     /locus_tag="QGF01_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJN20281.1"
                     /translation="MENFLLQLQSSDTVQSSLSENITNFVDRINPLQLPHETFVTDLA
                     IIMILAAVVTLAFFKIRQPLIIGYLFAGMLIGPLSPLWTSFLPEKVGPSTISGVGILS
                     DLSALNLFADIGVILLLFVIGIEFPFAKIRSIGRVAMGAGTLGLFATLGVVFLAASAL
                     GLNFMDSLFISAALSISSTAIIIKILQDAGKIKKESSILVLGVLIVEDVIAVILIASL
                     ESIGLVGAVSIENVVLVAAVAGGLIIGTFTIGRRIIPPLIDKVAAAENREILLLSVLG
                     VCFGYALLANVVGLSVAIGAFLAGVLIAESKSAEVSKILSSPIKDMFVAIFFVSVGAL
                     MDITQLENYIFIAFALIAVATAMKLGSNLFGNMLFKQPRNKSLRSAFALSAPRGEFSI
                     VIVKVGVDMGMVSAFLFPLIGLISIITAFMSPFIIRAGDKVILKLSKS"
     gene            4973..6235
                     /locus_tag="QGF01_05035"
     CDS             4973..6235
                     /locus_tag="QGF01_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HJN20282.1"
                     /translation="MTSKVNNPKAKSGKTNPNTSNILPLVKKVSTISDSTKLLSKEVK
                     AMSKIFTENQKILVSMKNMIDTLSLTIEQIQVQEKKLNLIEGDNERLFEGLEQVRNQS
                     NIISKINTQTNELQERVEKISKINENSPNSNELMEKIDLSFDSIKNNSKMIIKIADRV
                     ENVKEDLNNVSSKAEPISKITTKMENMTDNIRSMRQKTSDLGNNIKNFQKKLDSITSK
                     KNSPGDSGNLKNIKSEFITLRKYVGENSDELQDKILGLSDTLNRVNSSSAEFHEKSDS
                     IIQELQKIEKVTSKSSSSTSNEIIGLLKLSEYQSGIRMQSESKYGTLQDIEKMAQDTA
                     EIVNLFDSMSIETENKIPLPHEVRQWAISTIFDCADRWEISFSGLFKKMLELLGKDLL
                     KEAIRVQQVRDIFGIRAVDEIRNELGLT"
     gene            complement(6232..8262)
                     /locus_tag="QGF01_05040"
     CDS             complement(6232..8262)
                     /locus_tag="QGF01_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HJN20283.1"
                     /translation="MEPLTPNPFTSFIFDIFIACAIIIIAYTCNFYYLAFLSSRRNET
                     ENTVAIGEPTVTIHLPIYNEKYVAKRLIDAVCNLDYPKKFMRIMVLDDSDDDTTKLVS
                     ELVEDHKKNGFDILHVRRGTRTGYKAGALKHAMKSTESEFVAIFDADFIPPTWYLRKA
                     MPYFAKPSIGLVQCRWGHVNENYSALTQAQALSLDFHFLIEQRAKSNSRLFMNFNGTA
                     GIWRKTCIEDAGGWHTATLVEDLDLSYRAQMKGWKCLFIPDIVVDAELPVQMNGAKRQ
                     QFRWAKGSIQCAIKLLGGIIAKRKIAFDAKLQAFVQLTRHIVFPLMLIQFLTLPILLA
                     AEINLYIVSFLPILTLATYFAMGPGAYLYIIHNMYKKNWKEKALSMPYLIIYSIGMSV
                     NNTVAVFDAMIGKKNEFLRTPKYGIIKKTDDWRTKAYNLPFSQTTLLELFFGIYGILA
                     IFIAIYSSNPIWVPIIALQTIGFLYIAFMSFRHTRFKRDSSKTEHIQTKDEKMANITY
                     KLATVGIIAIICFGVYMGFTGYQENVYPIDLSIGLLDRIMASSEPKTIMADIQAMKEY
                     LPIEGNPVYMFPTDTTNFVRIQSDLDAMLISTEKISAVPRDSSAFHTGMMDVSLRAEV
                     VQENLMDVVPYMYASVSNILFSCVWIVGIIGIFAILKRKKQNIDIVDKSEGI"
     gene            complement(8327..9280)
                     /locus_tag="QGF01_05045"
     CDS             complement(8327..9280)
                     /locus_tag="QGF01_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJN20284.1"
                     /translation="MSLLLKDRVYTMQSPTAKRGVYPLHGYKLGLYRMPIKLEDPAEI
                     QSIFQGLKNTFEMDMYADRIYATYNWIEENMPDPDAKGYEQVKLSVTVEIVTGEVVDI
                     IYQIFSIEKFGDPQWVKDYRKKADHFAKMVIDTILRNTILSDKMVESLTSLEKISENQ
                     ALIKLEELTPLAQIVPDAKPIAKIEKPKPAVAGKKQVIVRDGEEAIPGPIDIEYKDKM
                     DQSTPFKTSTGNMVQTWGRVGADNKVMGVWGEFCSVDFDICVADGACIDACPVGVYDF
                     FEFSGNVASTKKPLQINEPDCIFCLACEGVCPPQAIKIYPQ"
     gene            9385..10200
                     /locus_tag="QGF01_05050"
     CDS             9385..10200
                     /locus_tag="QGF01_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HJN20285.1"
                     /translation="MKINKIGIVSKFGSKKSENAAKKIARKMLKMRSTVYTISPVSVT
                     GAKQLDSLDGLKKVKLDLVITLGGDGTTLRTFRNLENETPLLAINVGGNRGILSEIYL
                     NEIDNAIQCMKKNKVWLDKRKRVVASCNGVQFQPALNEIYVNRKNLTKTAEFETKFQN
                     DTVKQKMDGVIISTPSGSTGHSLSIGGPILHESLGVFIITPVAPVHRLPSIVVPDEKV
                     EVTCSHDCNIVMDAQVIKSAAFGNVITIEKYKKQAVFVRLKKKGLRQMSKLGF"
     gene            10190..10669
                     /locus_tag="QGF01_05055"
     CDS             10190..10669
                     /locus_tag="QGF01_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HJN20286.1"
                     /translation="MAFRVLDSSAFYAGIPFGSNEPSYTTSLVYDEIKHIKKNHDAVD
                     ILIETGRLTISNPESEFIEIVRNTANESGDLHELSVEDISIIALSLKLGAELITDDFA
                     VSNVAKNLNIQVFPVMTDGIKNIVSWTYYCPGCNNNFSESIKCPKCGNTLKRKNLKK"
     gene            complement(10671..11357)
                     /locus_tag="QGF01_05060"
     CDS             complement(10671..11357)
                     /locus_tag="QGF01_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HJN20287.1"
                     /translation="MNLKDLRIVVDEREKKSGIPDLLKSIGINVEMKMLPIGDYIVAP
                     ETIVERKSIQDLISSVFDGRLFDQCDRLKNNFEFPIILVEGNINAIEDLTENPFVFYG
                     AISSITIDFKIPIITTLDSSHTTKLLVAMCSQKAQSKGPFIKKIRKSNDIRKQQLSVL
                     CSLPGVGEKTAIQLLEKFGSPIRVLSSSTADLAKIKGLGKIRAIKIKKILQNQSKYLK
                     KSNQKTLHDS"
     gene            complement(11387..11785)
                     /locus_tag="QGF01_05065"
     CDS             complement(11387..11785)
                     /locus_tag="QGF01_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HJN20288.1"
                     /translation="MSSNQQIPPWLQEQIAKLQQTQQNLQMTVSQKQQLEFENIETEK
                     SLEELKKVSDDDAVFKFAGTILVKSNKQTLLEDLEEKKELIKTRTTVLAKQEDKLKTS
                     LKEQESKIQEMIKNPSAMGAKQPSEGESSQ"
     gene            complement(11809..12054)
                     /locus_tag="QGF01_05070"
     CDS             complement(11809..12054)
                     /locus_tag="QGF01_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HJN20289.1"
                     /translation="MTSSFNAKIEVDAKEKSQAIFDSINTDNEFYPENPTKTEMSFND
                     KIIIRIESNELTHMRANLNSCIRLIQTSYDSIKSVNI"
     gene            complement(12038..12259)
                     /locus_tag="QGF01_05075"
     CDS             complement(12038..12259)
                     /locus_tag="QGF01_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37"
                     /protein_id="HJN20290.1"
                     /translation="MAKLAKKKKSLSGLGARYGIKPRKQFTQVHRVLKSTRKCPECGS
                     LKFGREAVGIWSCKKCAFKVAGSAYDVKL"
     gene            complement(12261..13079)
                     /gene="rrp42"
                     /locus_tag="QGF01_05080"
     CDS             complement(12261..13079)
                     /gene="rrp42"
                     /locus_tag="QGF01_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HJN20291.1"
                     /translation="MTDFSIIDKLKRSKILDLLQEGKRIDGRALDEHRTLDVVTGVVP
                     HANGSARVRLGDTEVVAGVKIQPDRPFPDTGDRGIFICTAEILPLAHPSAETGPPQPP
                     VIELARVVDRGIRESGMIDLSQCVLEENKSVIGIFSDNVVTDHDGNLFDTCSYASVAA
                     IMTSKMPKWEMKDDKPVLVEGEEVDTPITTIPVSVTMGRIGEFILADPNIDEWACLDA
                     RITITTNSDGNIVALQKGGKDGFTYEQIVKCSEISISSGAKIREIIKKAVGETQ"
     gene            complement(13085..13816)
                     /gene="rrp41"
                     /locus_tag="QGF01_05085"
     CDS             complement(13085..13816)
                     /gene="rrp41"
                     /locus_tag="QGF01_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HJN20292.1"
                     /translation="MGGRKSDMVLLDENGIRCDGRKVNETRKVTIKAGVLKNASGSAY
                     IEFGGNKILAGVFGPRDVHPKHMSNPDTGILRVRYHMEPFSVGERKNPAPSRREIEIS
                     KVIKEALEPAVMLEKFPRTAVDVFLEILQADGGTRCAALDAASVALADAGIPMRDLVC
                     ACASGKAGDALILDVNNEEDQAGQADMPIGYMPNLGKITLLQLDGVLTPDEFNKCIEL
                     GVEGCKKVYEIQKNALREKYFSKGD"
     gene            complement(13818..>14101)
                     /locus_tag="QGF01_05090"
     CDS             complement(13818..>14101)
                     /locus_tag="QGF01_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HJN20293.1"
                     /translation="SGENLGKIDSGELVKVSPAKISYLISKHGSMIQTIESSTDTSIT
                     IGQNGLVVVSGSESNGLLKAIEAIQMVEEQAHLADLTDKVKKMLETNGD"
BASE COUNT         4657 a         2451 c         2254 g         4739 t
ORIGIN      
        1 caatccattt gttgccttcg ttatgcatac ctgtgattct accaaacaat tttttacatc
       61 tgcattgaat aactgttttt tcttttcttt aattgttgac aatagagtct gtaactccca
      121 ttgttcatcg aatactgtaa tatcatattc acaacaatca tatctaaaat catgattacg
      181 acatcctcca actccaatgg gaaattcttt tgtcaagagt ttctctatta tgtttttgat
      241 ttccaagcag aaatcataga attagatttt gtataaagtt tcaaacttgg aattattaac
      301 caatgatgta aatgacaaac cttagaaaaa ttatcaatta gtctaaaata caaatatatt
      361 tttattattg aatctgtttt gtttggcact atacccattc gtttaaattg tgattgattc
      421 tcgttttacc aagctcctgt ggtgtagtcc ggccaagcat actgccctct caaggcagtg
      481 atcccgggtt caaatcccgg cgggagcatt ttgtttttga ttgacctgct taaataattc
      541 aagagagttt taatgatatg acattacgtg atttgtcatt aaaacgtgaa tatagatcag
      601 acaaagatga tttactggcg gagttttttg ttccatgtct gactaattgt atccaatatg
      661 atagggcgat tgaattcata tcaataaaaa gcctcagtac actatctttt ggattagata
      721 gtataacaaa gaaacatgca aaaattagac taatttcagg tcatagattt agtacttctg
      781 atttgaacat gttaaaaaaa atctatcaga atggatatgg tcataaattt aatggtaata
      841 tgattcgaga tagcaaaata tctcatctgc agaaggtgat taaggacttt aaacttgaaa
      901 taaaaattgc aattccaaac tcagagctgg tagatggttc ttttgaagaa agattaggca
      961 tattcagaga tgagaatgat gatgtagttg cattcaccgg tacgtctaat gtcacatttg
     1021 atgcgcaaaa tagaaatttt gaatctattg acgtttttac atcatgggat gataaaatac
     1081 gtacagatgc caaaattgaa aattttgaaa agctttggaa taatgaaaca cattatgttg
     1141 aagtatatga tcttatattt gcagaaaaaa ataatctttt aaaatataca acggaatggg
     1201 cagtggataa gtattaacta gaaatcaaag tcatccatgt ttattttttc aatattttgc
     1261 tgaaacttga ttcttgaaat cttataatag attatttctc cacggcgttc aatcactgca
     1321 attatgggtt ccttacccat gcttgtaatc tcttgaatct ttttttgtag ttttcctacc
     1381 ttttcctgct taccctcatt tagaccaaaa actagaaact tggcattttt ttctccaaaa
     1441 tctcccttat tgtagaccct aaaatcagat ccaaaaccaa atccatcttt aaccgtataa
     1501 ccccttgtcc taagatcacg ataaatcaaa aaattagtta gtatattctc atctaactgt
     1561 ctatagatct ctaaaagaaa gtcaaagttc aaattctttt ttcctttaaa aattactagt
     1621 tttccaacat gtaagagata aagtgattcg aatggtttta aaatcaattt gttttctttc
     1681 atttcaccat aaccctttag attcagatca tgctgaatgt ctggatctaa aatcaaagtt
     1741 tgatttttta tgaaaacacc ctgtatcttt gaatcacttt caaaagccat caaaaatttt
     1801 gttataactg agcatataag atgtatgaag actatcgtta aaatatgggc ataaaggcat
     1861 caaagaatta tgcatggtga atgttataga agaggtaatg gtcaaccgta caccagaaag
     1921 gagtacataa aaggtaaacc acaaatcaaa atagccaaat ttcatggtgg acaaaaggga
     1981 aaatatgatt tttctgttca actgatgcta aacgagaaac ttcagatcag acatatggct
     2041 atagagtcaa ctagactggc agccaataag acgcttgaaa agacagtggg tgaaacaggc
     2101 tattggtcaa gacttagaat ttaccctcat gttttacttc gagagaataa aatgattgca
     2161 gctgctgggg ctgatagatt acaagaggga atgcgaagag cctttggaaa agcagttagt
     2221 ttaggggcac gtgtcaaagc gggtcaaatc ataatggaaa tgcatgttaa aaaagagcat
     2281 gttgatgcag caacaaaggc attaaaatct gcatgtgtaa aattacctgg gacagcaaaa
     2341 attaatgtag ttaaactata agatcagaaa gacaaaccca agaattcttt ttcatgttgt
     2401 tttaatatct gaatgaattc agaaaattct tcttccgttg gatccctctt tacaattctt
     2461 ctatattgat aataaaatga attatagagt cttccaaata ttatcccgta tctgaatgaa
     2521 tttttatctg atgacagcaa ttccgatgaa tttgtgatag agttaatttc atttttctta
     2581 tcaaatatct cgagaatttt ttcttctaaa actttttgga ctttttcatc catattaaat
     2641 ataataataa tatagttaaa aagaatccgt atcaatcatt acgctttaat agacattaac
     2701 aaaattatcg atcaagcagt tgtagtatag cttggttatt atgcgagctt gccaagttcg
     2761 tggcccgggt tcaaatcccg gcaactgcat tcttttatga tttcaaattt tgatggtttg
     2821 tttgcaaacc agtttttatt atatctaaag catttacagc tctttctggt ttgggtaatt
     2881 gaaccatgaa gactttgtct tggttgactt gagagtgatc tggaggagat aatgcaaaca
     2941 ttgtagtatc tgccaatgcc tcaaaaaaat taagcgtgtc attggcataa atcagaaatt
     3001 tgttatgtat gtctccatga gaaaaatcta atgttatttc aacaaacacg tggcctaacc
     3061 catcctgtaa tatgttaaga ttagtatcaa cagagattgg tttaccttgt atttttaaca
     3121 tcatattatc aaattgtcta tcagataaaa taattcttgg aacatttttt ccatcaaaat
     3181 caaaaatatc tatatcctta tgtacacgtt ctaacatttg ttctaactct ttatttgcca
     3241 tatcctagga tttagaaagt ttcagaatga ctttgtcacc tgcacgaatt atgaatggag
     3301 acatgaatgc cgtaatgata gatatcaagc ctatcaatgg gaacaaaaag gcactaacca
     3361 tgcccatatc cacacctact ttcacaatta cgattgaaaa ttctccacgc ggagcagata
     3421 atgcaaacgc agaccgtagt gacttatttc ttggttgttt gaatagcata tttccaaaaa
     3481 gattgctacc caatttcata gctgtagcaa ctgcaatcaa tgcaaatgcg atgaaaatgt
     3541 aattttctag ttgagtgata tccatcaatg caccaacaga aacaaagaag attgctacaa
     3601 acatgtcttt gattggactt gataaaattt ttgacacttc cgcagatttt gattctgcga
     3661 tcaaaactcc agctaaaaat gcaccaattg caacagaaag tcctactaca tttgctaaaa
     3721 gtgcgtaacc aaaacaaaca ccaagaacac taagtagtaa aatttctcta ttttccgcag
     3781 ctgcaacttt gtcgattaat ggaggaatta ttcgtcttcc aatagtaaat gtaccaataa
     3841 ttagaccacc tgctacagca gctaccaaaa caacattttc aatcgatact gctccaacta
     3901 aaccaattga ttcaagtgat gcaatgagga ttactgcaat tacatcttca acaatcaaga
     3961 ctcccaaaac aagtatagac gattcttttt ttattttgcc agcatcttgg agtattttga
     4021 taattattgc tgtactgctg attgataatg ccgcagatat gaataaggaa tccataaagt
     4081 ttagaccaag tgcagatgct gcaagaaaga ctacgcctaa tgtggcaaaa agccctaatg
     4141 tacctgcacc cattgcaact cttccaattg atcgaatctt tgcaaatgga aattctatgc
     4201 caattacgaa aagtaataaa atcacaccaa tatcagcaaa gagattcaac gctgatagat
     4261 ctgataatat gccaacacca ctaattgtac tgggtcctac tttttcaggc aaaaatgatg
     4321 tccacaatgg tgataatgga cctatcaaca ttccagcaaa caaatatcct ataatcaatg
     4381 gctgtctgat tttgaaaaat gctagtgtaa ctacggcagc taaaatcatt atgattgcaa
     4441 gatctgtcac aaaagtttcg tgcggtaatt gtaaaggatt gattctatca acaaaattgg
     4501 tgatgttttc actcaatgaa gattgaacag tatcacttga ttgtaattgt aaaagaaaat
     4561 tttccatcca ttatttactc atatttattt aaaaacgatt tgatgtaatg cataggtcaa
     4621 ttagtcacaa tcagtcatga tagattttac ataaatttat ttttgaaata atttacaaca
     4681 aaatgggatg atgagcagag tgactgatgt catgaagaaa taagctctgg ggtatctgac
     4741 caatcaatga gtgatttcac aattacccac actattaata gtaccgtacc ataccgtaat
     4801 atatgataca atgtgagtat tgttcaagag gttttgtaga tactatgaat ggtttagctg
     4861 aaaaaacatt tcatgaagta ttacatgctc caaaaatagt caatcagtaa aatccaatcc
     4921 ttctattttt ttttagcgag ctttttaaca acactaaaag ttcaggatta gtatgacatc
     4981 taaggtaaat aatcctaaag caaaatctgg gaaaaccaac ccaaacacat caaacatttt
     5041 accactagta aaaaaagtat caactatttc agattctacc aaattattat caaaagaagt
     5101 aaaagctatg tcaaaaattt ttaccgaaaa tcaaaagatc cttgtatcaa tgaaaaatat
     5161 gattgataca ctcagtttaa caatagaaca aatccaggtt caagaaaaaa aactcaacct
     5221 tattgagggt gataatgaaa gattatttga aggattagaa caagtaagaa atcaatcaaa
     5281 tattatttcc aaaattaata cacagacaaa tgaattacaa gaacgggtag aaaaaatatc
     5341 taaaatcaat gagaattctc ccaactccaa tgaattgatg gaaaaaatag acctgtcatt
     5401 tgattccata aaaaataatt caaaaatgat aatcaaaatt gctgataggg tagaaaatgt
     5461 gaaagaggat ttgaataatg tgtcttcaaa agctgagcca atttcaaaaa ttacgacaaa
     5521 aatggaaaat atgacagata atattagatc tatgaggcaa aaaacctcag atctgggtaa
     5581 taatataaaa aatttccaaa agaaattaga tagtattact tcaaagaaga attctccagg
     5641 ggattctgga aatcttaaaa atataaaatc tgaatttata actttgagaa aatatgtggg
     5701 ggaaaattct gatgaattac aggataaaat tttaggttta tctgacacgc tcaatagagt
     5761 aaattcatct tctgctgaat ttcatgagaa gtcagattct ataattcaag aattacaaaa
     5821 aattgaaaag gtgactagca agtcatccag ttcaacctca aatgaaatta ttggactttt
     5881 gaaattatca gaatatcaat cgggcatacg tatgcagtct gaatcaaaat atgggacatt
     5941 acaagatatt gaaaaaatgg cccaagatac agcagagatt gttaatcttt ttgatagtat
     6001 gtcgattgaa acagaaaata agattccgtt accgcatgaa gtaagacaat gggcaataag
     6061 cacaattttt gattgtgctg atagatggga aattagcttt tctggtctat ttaagaagat
     6121 gttagaatta ttgggtaaag atttgttgaa agaagcaatc cgggtacaac aagttagaga
     6181 tatctttggt attagagcag ttgatgaaat tagaaacgag ttaggcctta cctagatacc
     6241 ttctgattta tcaactatgt caatattctg ctttttcctt ttcaagattg caaatattcc
     6301 aatgattccc acaatccata cacaactaaa aagaatattt gaaacacttg catacatgta
     6361 tggcactaca tccataagat tttcctgaac cacctcagcc ctaagactga catccatcat
     6421 cccagtatga aatgctgagc tatctcttgg aacagcagaa attttttcag tgctaattaa
     6481 cattgcatct agatctgatt gtattctaac aaagtttgtt gtatcagttg gaaacatgta
     6541 taccggattt ccttctatcg gtagatattc tttcattgct tgaatatctg ccataatggt
     6601 ttttggttca gaggaagcca tgattctgtc aagtaaccca attgatagat caataggata
     6661 aacattttct tgatatccag taaatcccat atatacgccg aagcatatta tagcaataat
     6721 tcccactgtt gcaagtttgt atgtaatatt tgccatcttt tcatcctttg tttgaatatg
     6781 ctcagttttt gaactgtctc ttttaaacct tgtatgtctg aaactcatga atgcaatata
     6841 caaaaagcct atagtttgta aagcaattat aggaacccag attgggtttg aggagtaaat
     6901 tgcgataaag attgcaagta ttccataaat tccaaagaaa agttccagta atgtagtttg
     6961 agagaatggt aaattataag ccttagttct ccaatcatca gtttttttga tgattccata
     7021 ttttggagtt cgtagaaatt catttttctt tcctatcatg gcatcaaata cagctacagt
     7081 attgttaact gacattccta tagaataaat tatcaagtat ggcatagaca gagccttttc
     7141 tttccaattt tttttataca tattatgaat tatgtacaga tatgccccag gtcccatagc
     7201 aaaatatgtt gccagagtca gtatgggtag aaaactaaca atgtaaagat tgatctctgc
     7261 tgctagaaga attggaagtg taagaaattg gattagcatt aagggaaata ctatgtgcct
     7321 agtaagttgt acaaacgcct gaagttttgc atcaaatgca atctttcgtt ttgctataat
     7381 accacccaaa agttttatgg cacattgaat agagcccttc gcccaacgga attgttgtct
     7441 tttggcacca ttcatttgca caggtaattc agcatcaact acgatgtcag gtatgaaaag
     7501 acatttccaa cctttcattt gtgctctata actcaaatcc aagtcttcta caagtgttgc
     7561 agtgtgccat cctccagcat cttcaataca tgttttcctc catattcctg cagttccatt
     7621 aaaattcatg aataaacgtg aattactttt tgccctttgt tctatcaaaa aatgaaaatc
     7681 aagacttagt gcttgtgctt gagttagcgc agaataattc tcatttacat gtccccatct
     7741 acattgtaca agcccaatgc ttggctttgc aaaataaggc attgcttttc tcagatacca
     7801 tgttggtgga ataaagtcag catcaaaaat tgcaacaaac tcactttctg tggatttcat
     7861 agcatgtttg agtgcacccg ccttgtatcc agttctagtt ccacgtctga catgtaatat
     7921 atcaaatccg tttttcttat gatcttctac aagttctgat acaagttttg ttgtatcatc
     7981 atcagaatca tctagaacca ttatacgcat gaatttttta gggtagtcta gattacaaac
     8041 tgcatctatc aaacgctttg ccacatattt ttcgttatag attggaaggt gtatagttac
     8101 agttggttct ccaattgcaa ctgtattctc agtctcgttc ctacgacttg aaagaaatgc
     8161 caagtagtaa aaattacatg tatatgcaat tataataatc gcacaagcga taaaaatatc
     8221 aaaaataaaa gatgtgaatg ggtttggtgt taatggttcc attttataat attatcaaaa
     8281 tttagttcaa tattaacagt tatggaattt actatagaat ccaaacttat tgtggataaa
     8341 ttttgattgc ctgtggtgga caaacacctt cacatgcaag acagaagata caatctggct
     8401 cattaatttg aagtggtttc ttagtagatg caacgtttcc tgaaaattca aagaaatcgt
     8461 aaacacctac aggacatgca tcaatacaag caccatctgc tacacagata tcaaaatcaa
     8521 cagaacaaaa ttctccccaa acacccatga ccttgttatc cgcaccaaca cgtccccaag
     8581 tttgaaccat attacctgtg gaagttttga atggtgttga ttgatccatc ttatctttat
     8641 attctatatc aataggaccc ggaattgctt cctctccatc tctcactatt acttgttttt
     8701 tacccgcaac tgctggcttt ggtttttcta ttttggctat tggttttgca tctggcacaa
     8761 tttgtgctag tggggttaat tcttctaatt ttatcaatgc ctgattctca gaaatttttt
     8821 ctaagctagt aagtgattca accattttat cagagagtat agtatttctg agaattgtat
     8881 caattaccat ttttgcaaaa tggtctgcct tttttctata atctttaacc cactgaggat
     8941 caccaaattt ttcaattgaa aatatttgat aaataatatc aactacttca ccagttacaa
     9001 tttcaacagt aacactaagc ttgacttgtt catacccctt cgcatcagga tctggcatgt
     9061 tttcttcaat ccaattataa gttgcataaa ttctatcagc atacatatcc atctcaaaag
     9121 tattttttag tccttgaaag attgactgta tctcagcagg atcctcaagt tttattggca
     9181 ttctgtataa tcccaatttg taaccgtgta atggataaac tcctcttttt gcagtgggtg
     9241 attgcattgt gtaaactcta tccttcaaaa gtagggacat agttcaatta tggtcaaatt
     9301 tagttaaaaa tctttgtttg tgagactatt agcatgatcc tcaaaaaatt aaatttttaa
     9361 gaagcttttg taaaaaagat ccgcttgaaa atcaataaaa ttggaattgt tagtaaattt
     9421 ggttcaaaaa aatctgaaaa tgcggcaaaa aaaatagcca gaaaaatgct aaaaatgaga
     9481 tctacagttt atactatttc tcccgtatca gtaacaggag caaagcagtt agattcgtta
     9541 gatggtttga agaaagtaaa acttgatctt gttataacat tgggtggtga cggaactact
     9601 cttagaactt ttagaaatct tgaaaatgaa actccacttc tagcgataaa tgttggtggg
     9661 aacaggggaa ttttatcaga aatatatttg aatgaaattg ataatgcaat tcaatgcatg
     9721 aaaaaaaata aggtttggct tgacaaaaga aaacgagttg tagcttcttg taatggtgta
     9781 caatttcaac cagctctaaa tgaaatttat gttaatcgta aaaatttaac aaaaactgct
     9841 gaatttgaaa ccaagtttca aaatgataca gttaaacaaa aaatggatgg ggttatcatt
     9901 tctacaccta gcggttctac cggtcattca ttatcaattg gaggtcctat acttcatgag
     9961 agtttaggtg tattcattat cacaccagtt gctccggttc atagacttcc ttcaattgtt
    10021 gttccggatg aaaaagtaga ggtaacatgt tcacatgatt gtaatattgt gatggatgcg
    10081 caagtcatta aatcagctgc ttttggaaat gtcataacca tagaaaaata caaaaaacaa
    10141 gctgtctttg ttaggttaaa aaagaaagga ttgaggcaaa tgagtaaact tggcttttag
    10201 ggttttagac tctagtgcat tttatgcagg aattccattt ggttcaaacg aaccaagtta
    10261 tactacatca ttagtgtatg atgaaattaa acatattaaa aaaaatcatg atgcggttga
    10321 tattttaatt gaaacaggga gattaactat ttccaatcca gaatctgaat ttattgaaat
    10381 agtaaggaat actgcaaacg agtctggtga tttacatgaa ctatctgtag aagatatttc
    10441 aataattgca ttaagcctga aacttggtgc cgaactcata actgacgact ttgcagtttc
    10501 aaatgtagca aaaaatctca atatacaagt ttttccagta atgacagatg gcataaaaaa
    10561 tattgtatct tggacatatt attgtcctgg atgtaacaat aatttttctg aaagcataaa
    10621 atgcccaaaa tgtggaaata cactaaaaag aaaaaattta aaaaaatgat tcaggaatcg
    10681 tgaagtgttt tttggttact tttttttaaa tatttacttt gattctgtag tatttttttt
    10741 atttttattg cacgaatttt tcctaaccct ttgatctttg caagatctgc agttgatgaa
    10801 cttaggactc gtattggact accaaatttt tctaatagtt gtatagcagt tttttcccct
    10861 acaccaggta aactgcacaa aacagaaagc tgttgtttcc gtatatcatt tgattttcta
    10921 atttttttga taaatggtcc cttggattgc gctttttgtg aacacattgc aactaataat
    10981 tttgtagtgt gagatgaatc aagtgtagtg attattggaa tcttaaaatc aattgtaata
    11041 gatgaaattg ccccataaaa tacaaatgga ttttctgtta gatcttcaat tgcattaata
    11101 tttccttcca ctaggatgat agggaattca aaattattct tcaatctatc acattgatca
    11161 aataatcttc catcaaacac agatgaaatt agatcttgga tactttttct ttcaactata
    11221 gtttcaggtg ctacaatata atctccaatt ggtaacattt tcatttcaac atttatgcca
    11281 attgatttca aaagatcagg tataccactt tttttctctc tttcgtctac aactatacgt
    11341 agatctttta aattcataaa taatatcagt attttttatt aatattttat tgggaagatt
    11401 cgccttctga tggttgttta gcacccatag cagatgggtt tttgatcatt tcttgaatct
    11461 tagattcttg ttccttcagg ctagttttta gcttatcctc ctgttttgct aaaacagtag
    11521 ttctagtttt tattaattcc tttttttcct caagatcttc aagaagtgtt tgcttattcg
    11581 attttaccag tatagtacca gcaaatttga aaactgcatc atcatcagat acttttttta
    11641 actcttccaa tgatttctcg gtttctatat tttcgaattc aagttgctgt ttttgtgaaa
    11701 cagtcatttg gaggttttgc tgtgtttgtt gtagctttgc tatttgctcc tgtagccatg
    11761 gtggtatttg ttgattggat gacatgtaat tatacaaata atgtttgatt atatatttac
    11821 tgatttaatc gagtcgtaac ttgtttgaat aaggcgtata catgaattaa gattagcacg
    11881 catatgcgtt aattcatttg attcaattcg aattatgatt ttatcgttaa aagacatctc
    11941 agtttttgtt ggattttcag gataaaactc gttatctgta ttaattgaat caaagattgc
    12001 ctgtgatttt tcttttgcat ctacttctat ttttgcatta aagcttgacg tcataagctg
    12061 agcctgccac tttgaatgcg cactttttac aactccagat ccctacagcc tcacgaccaa
    12121 atttcagtga cccgcattct ggacatttcc ttgtagattt cagtacacga tgaacttgtg
    12181 tgaattgttt tctaggtttg attccatacc gagcaccaag accggaaagt gattttttct
    12241 ttttggcaag ttttgccatg ttattgggtt tctcctactg ccttcttgat aatctctctg
    12301 atttttgctc cagatgaaat agatatttcg gaacacttta caatctgttc atacgtaaat
    12361 ccatccttac cacctttttg aagtgcgaca atatttccat ccgagtttgt tgttattgta
    12421 attcgtgcat ctagacatgc ccattcgtca atatttggat cagccaaaat aaattcccct
    12481 attcgtccca ttgtgactga tacaggtata gtagttattg gagtatctac ttcttctcct
    12541 tcgaccaaaa ctggtttatc atctttcatc tcccactttg gcatctttga agtcatgatt
    12601 gctgctactg aagcatatga gcatgtgtcg aacaagtttc catcgtgatc agttaccaca
    12661 ttatctgaga atatacctat aacggattta ttttcttcca aaacacattg agataaatca
    12721 atcatgccac tttcacgtat gcccctatca acaactcgtg caagttctat gacaggtggt
    12781 tgaggagggc ctgtttctgc acttgggtgt gccaatggca gaatttccgc tgtgcagatg
    12841 aaaatacctc tatcacctgt atcaggaaaa ggtctatcag gttgaatttt tacaccagca
    12901 acaacttctg tatcacctaa tctaactcgt gcagaaccat tagcatgtgg taccactcct
    12961 gtgactacat ccaaggttct atgctcatca agtgcacgac catctatcct ttttccctct
    13021 tgaagtaaat cgagaatttt agatcttttt agtttatcta taattgaaaa atctgtcatt
    13081 gttatcaatc acccttagaa aaatattttt cccgtaatgc atttttctgt atctcgtaga
    13141 cctttttaca tccctctacg cctaattcaa tacatttgtt aaattcatca ggtgttagaa
    13201 caccgtctaa ttgtaaaagt gtgatttttc ctaagttagg catatatcca atgggcatat
    13261 cagcttgtcc tgcctgatct tcttcattat ttacatcaag tatcaaagca tctccagctt
    13321 ttcctgaagc acatgcacaa actaaatctc tcataggaat tccagcatca gccaacgcaa
    13381 cagatgctgc atcaagtgca gcacatctag ttccaccatc agcttgtaaa atttcaagaa
    13441 atacatctac tgcagttcta ggaaattttt caagcattac agcaggctct aatgcctctt
    13501 tgataacttt gcttatctct atctcacgtc tagatggagc tgggtttttt ctttcaccaa
    13561 cagaaaaagg ctccatgtga tatctaactc tcaaaattcc tgtatctgga tttgacatat
    13621 gtttaggatg aacatctctt ggtccgaaaa cacctgctaa tattttattt ccaccgaatt
    13681 caatgtatgc agaaccgctt gcatttttta atactccagc ttttatcgta acttttcttg
    13741 tttcatttac cttacgacca tcacaacgaa tcccattttc gtcaagtaat accatatctg
    13801 attttcttcc acccaaatta atcaccatta gtttctaaca tttttttcac tttatctgtc
    13861 aaatcagcaa gatgtgcctg ctcttcaacc atttggattg cttcaattgc ctttaataac
    13921 ccatttgatt cactgccgga tacaaccacg aggccatttt gacctattgt gatagatgtg
    13981 tcagtagagg attcaattgt ttgtatcata gaaccatgtt tagatataag ataagaaatt
    14041 tttgcaggag agacctttac taattcacct gaatcaatct ttcctaaatt ttcaccagat
    14101 a
//
LOCUS       DASCPJ010000105         3190 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001688688, whole genome shotgun sequence.
ACCESSION   DASCPJ010000105 DASCPJ010000000
VERSION     DASCPJ010000105.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001688688"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(457..1374)
                     /gene="trxB"
                     /locus_tag="QGF01_05095"
     CDS             complement(457..1374)
                     /gene="trxB"
                     /locus_tag="QGF01_05095"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HJN20294.1"
                     /translation="MHDVIIIGAGPAGYTAGIYCSRARHDTLLISGILPGGQLMNTTD
                     VENYPGFSDGIMGPDLMTVMRKQTEKMGTKIIDDEVTSVDFKNRPLKVKTASEEFEAR
                     AVIVCTGANPRKIGLEGEQTFAGKGVSYCATCDGAFFRDQELIVVGGGDSAMEEAIFL
                     TKFASVVHIVHRKEEFRASKIMQERALSNEKIKVHFNSTVKEIHGDQKFQQVVLNNTK
                     TNEDKILEAGGLFIAIGHEPNSAIFQDQIELDGNGYIIIKNNTETNVTGVFCAGDVHD
                     HRYRQAITAAGFGCMAAIDVDKYLSESNT"
     gene            complement(1399..2208)
                     /locus_tag="QGF01_05100"
     CDS             complement(1399..2208)
                     /locus_tag="QGF01_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6775 family putative metallopeptidase"
                     /protein_id="HJN20295.1"
                     /translation="MNFSKIILYDEPAVSELEINRLQKFLVNTFHVDVTVQNNLFNDV
                     NDDIIEEIASCRVFDLKSTFKKYKPNNEQVNFERNYCKNTKFIEEIPTQETAENLDDL
                     VIYDGFEIKNILESVIPKDETANDCLHIIFTNKLICTYDYGDFRYHGRAVICSNPSII
                     STSGIVEAPAKSREYYLDVMKAKSQMHDLKSIKKKYQNTFLDYHDKRTSKIIEGYLLQ
                     AIFYYITGDPFCENIDCRLNNAHWQKDLLYSQLEFSKLCKKHHSILEKLHL"
     gene            complement(2205..3044)
                     /locus_tag="QGF01_05105"
     CDS             complement(2205..3044)
                     /locus_tag="QGF01_05105"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HJN20296.1"
                     /translation="MQESVNEQRPQKIFADAKEVEITRAIAEEFFSVLQDRAESDVII
                     IGAGPAGLTAARELSLMGYKILVIEQNNYLGGGYWLGGYMMNPVTVRAPAQKIWDELG
                     IPYKKVGDGLYLTPGPHAVSKLIAATCDAGVKFLNLTKFDDLVLRHGRVAGIVVNWMP
                     VSALPRNITCVDPIALEAKMIIDASGHDSVAVKRLLDRKMVDWKGMNPMWVENGEEHV
                     VEKTGEVYPGLIAAGMSVTETHGLARMGPTFGSMLYSGKRAAEVADQKIKEFDNKIPN
                     KSD"
BASE COUNT         1026 a          637 c          424 g         1103 t
ORIGIN      
        1 taaagcaaaa gcaaaaccta aagcaaaagc aaaacctaaa gcaaaagcaa aacctaaagc
       61 aaaagcaaaa cctaaagcaa aagcaaaacc taaagcaaaa gcaaaaccta aagcaaaagc
      121 aaaacctaaa gcaaaataat ctctagatag tatttaacta gaaaagatag tatcattcat
      181 actactaatt tcttagtgcg ccttttattt gatttacatc tagcatgatt gatgaataaa
      241 atcaaatctt ccatgatttt tacacatcaa atcatgttat actaacggga caagtcctaa
      301 cccgttttta tttttttatc cagtttttat tatgacattc acatctacaa ctttcattgc
      361 aaaaacatcc agcacagtat gaacaacgta ccttaaacat agcccattat tacaaaattt
      421 gtatttaagt ggatttttct atatgttata gatagttcaa gtattagact cactcagata
      481 cttatcaaca tcaatagcag ccatacatcc aaatccagca gcagtgattg cctgtctata
      541 cctatgatca tgtacgtcac cggcacaaaa aacaccagtc acattagttt cagtattatt
      601 ttttataatt atgtaaccat ttccatctaa ttctatttga tcttggaata ttgcactatt
      661 tggctcatga ccaattgcta taaacaatcc tccagcttcc aggatcttat cttcgttagt
      721 ttttgtatta tttagaacca cttgctgaaa tttttgatca ccatgaattt cttttacagt
      781 agaattaaaa tgaactttaa ttttctcgtt tgataacgct cgctcctgca taatcttact
      841 tgctctaaat tcttcctttc tgtgtacaat atgtactact gaagcaaact ttgtaaggaa
      901 aattgcctct tccattgctg aatctccgcc accaacaaca atcaattctt gatctctgaa
      961 gaatgctcca tcgcaagtag cacaatatga aactcccttt ccagcaaatg tttgttctcc
     1021 ttccaatcca atttttcttg gattcgcacc agtacataca atcactgccc ttgcttcaaa
     1081 ttcctctgat gcggtcttca cttttagtgg tctattttta aaatctacag aagttacctc
     1141 atcgtcaata atttttgttc ccattttctc tgtctgcttt ctcataacag tcatcagatc
     1201 tggacccata ataccatcag aaaatccagg gtaattttca acatcagtag tattcattaa
     1261 ctgaccacca ggcaatatgc cagaaatcaa taatgtatca tgtcttgccc ttgaacaata
     1321 aatgccagca gtatatccag ctggtcctgc accaataatt atgacatcat gcatacttta
     1381 ttgaaagtca gtggtagttt aaagatgtag tttttcaagt attgaatgat gtttcttaca
     1441 aagtttgcta aattctaatt gtgaatataa tagatccttt tgccaatgag cattgtttaa
     1501 tctacaatca atattttcac agaatggatc accagtaata taataaaaaa tagcctgaag
     1561 taagtaacct tctattattt ttgatgttct tttatcatga taatcaagaa aagtgttctg
     1621 atactttttt ttaattgatt ttaaatcatg catctgtgat ttggctttca ttacatcaag
     1681 atagtattca cgtgattttg caggagcttc gactatgcca gatgttgata taattgaagg
     1741 attagaacat atcaccgctc taccgtgata tctaaaatca ccataatcat atgtacatat
     1801 cagtttgtta gtaaaaatta tgtgaagaca atcatttgct gtttcatctt ttggtatcac
     1861 actttcgaga atatttttaa tttcaaatcc gtcatagatt acaagatcat ctagattttc
     1921 tgcagtttct tgtgtaggta tttcttcgat aaattttgta ttcttacaat aatttctctc
     1981 aaaatttacc tgctcattat taggtttgta ttttttaaat gtagatttca gatcaaaaac
     2041 tctgcacgag gcaatttctt ctatgatgtc atcattaaca tcattgaaaa gattattttg
     2101 cactgtaaca tccacatgaa atgtgtttac taggaatttt tgtaatctat taatctctaa
     2161 ttctgaaact gctggctcat catataatat aattttggaa aagttcaatc agacttattt
     2221 ggtattttgt tatcaaactc cttgattttc tgatctgcaa cctcggcagc tcttttacca
     2281 gaatatagca tagacccaaa tgttggaccc attcttgcta gaccgtgagt ttcggtaact
     2341 gacattcctg ctgcaataag accaggataa acttctccag ttttttctac aacatgctct
     2401 tcaccatttt caacccacat tggattcata cctttccaat caaccatttt tctatcaagt
     2461 aatcttttta cagctactga atcatgccca gatgcgtcta tgatcatttt tgcctctaat
     2521 gcgattggat caacacatgt aatgtttctt ggcaatgctg ataccggcat ccaatttacg
     2581 acgatacctg caactcttcc atgtcttaaa actaaatcat caaattttgt aagattcaaa
     2641 aattttactc cagcatcaca tgttgctgca attaattttg atactgcatg tggaccagga
     2701 gttaagtata gaccatcacc aacttttttg taaggaattc caagttcatc ccaaattttc
     2761 tgtgcgggag ctctaactgt aactgggttc atcatataac cacccaacca ataacctcca
     2821 ccaagatagt tattctgctc aataactaaa attttgtagc ccatcaatga taattcacgc
     2881 gcagctgtta atccagcagg tcctgcaccg ataattatca catcagattc agctctatct
     2941 tgtaaaacag agaagaattc ctcagcaatc gcacgtgtta tttcaacttc ttttgcgtca
     3001 gcaaaaattt tttgcggtcg ctgttcatta actgattctt gcacaaaaat acaatataat
     3061 tagtgcatat aatttttctt gtttgaaaat taggcaacac taatggagct agttggcaat
     3121 tattacctaa attattcatt atactagtta aaattcctat tatcaaaaat ttatatccgt
     3181 agtttaataa
//
LOCUS       DASCPJ010000106         9081 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001688773, whole genome shotgun sequence.
ACCESSION   DASCPJ010000106 DASCPJ010000000
VERSION     DASCPJ010000106.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001688773"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="QGF01_05110"
     CDS             complement(<1..387)
                     /locus_tag="QGF01_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HJN20297.1"
                     /translation="MNSKAIITSALPYANGEIHLGHVASTYLPADVTTRFLKQKGVEA
                     YYICASDDFGTPILIQSEKEGKTPEEYVKYWNKRDAEDFAAFNIEFDFFYRTSSPENK
                     EFVQYVFKKLENAGHIYENDIIQFYCN"
     gene            complement(423..1661)
                     /locus_tag="QGF01_05115"
     CDS             complement(423..1661)
                     /locus_tag="QGF01_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HJN20298.1"
                     /translation="MSKVKDKKLAKKGKISYEWARSHMQILDNTIMRLKKSKPLKGIT
                     LGFCLHITKETSVLLMGAKELGATVACCGGNPLTTQDDIAAFLASQGIHTYCWAGQSP
                     KEYDWCIQQVLNHKPQILTDDGSDMNVKAHFDKKYNSLKIFGATEETTAGVNRIRAVE
                     KQGKLRYPVISVNDAYTKHMFDNKYGTGQSTIDGYLRAMNLLFASKRVVVAGYGWVGK
                     GVAANCRGMNAKVIVTEVNPIRALEAHMDGFDVMPMAQAAKIGEIFITTTGMTSIIRK
                     EHILKMKDGAVLGNVGHFDVEVDSKFLLKQSKSVRQVRPYLDECVLKNGKKVYLIGEG
                     RIANLTAAEGHPPEVMAQSFSNQLLSILYILKNHKKMPKKVVTVPSEIDKQVAIDALK
                     AMNVKIDTLTKEQIKYMSSW"
     gene            1743..2045
                     /locus_tag="QGF01_05120"
     CDS             1743..2045
                     /locus_tag="QGF01_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HJN20299.1"
                     /translation="MTWKKITEKSTIVSGKGMEFEIDGKKIAVFNQNGYHALDALCVH
                     QDGSIAPGKLDGDIVECPLHFWHYNIKTGELKDFLKDIKLQTYKTDVRDDGVYIDL"
     gene            complement(2050..2277)
                     /locus_tag="QGF01_05125"
     CDS             complement(2050..2277)
                     /locus_tag="QGF01_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20300.1"
                     /translation="MKGKLIIVGAILIGGGMLLFPDTIEQIDIPSLAHAALDDAENLQ
                     NDPAVQDNFNNIIQIFYEKLDVLKSLFIDSL"
     gene            2391..3179
                     /locus_tag="QGF01_05130"
     CDS             2391..3179
                     /locus_tag="QGF01_05130"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HJN20301.1"
                     /translation="MNHEILREIITQDYVKIQNDIEKFLKDSVLENKADGVIFGLSGG
                     IDSAIIAYLSAKIFGKKALALVMPDSTVSPSSETGDALKIIGELGIDYKLIDIDVIHK
                     IYSNYLEPEKRALGNLRARIRANIIYYYANLKNLLVLGTSDKSEYSIGYFTKFGDGCA
                     DLLPISNLYKTQIREFAKILGIPDNIITKKSSPNLWKDHDAEEEIGISYEEIDSILYC
                     IEKKISVDEVAKMTEIKKEFVEKIIQMHENSKHKRLPPVGINEG"
     gene            3187..4575
                     /gene="cysS"
                     /locus_tag="QGF01_05135"
     CDS             3187..4575
                     /gene="cysS"
                     /locus_tag="QGF01_05135"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HJN20302.1"
                     /translation="MQIFDTLNNTKTELKFLDKVTIYLCGVTVYDDAHIGHARTIIVF
                     DVLRRFLESQKNIVEIVQNFTDVDDKIIARANEKKVSTVELTSKYTENYFRDFDKLNV
                     KRATHYPKATEHIEDMLNLISQLIDKKCAYITKNGVYFSVSKFRDYGKLSKKNIKELI
                     SGSRVMVDEEKNDPLDFALWKFSDTQPIWNSPWGKGRPGWHIECSAMSRKYLGDGFDI
                     HGGGRDLIFPHHENEIAQSESSTSNQFAKIWMHVGMITINGEKMSKSIGNVKSVSHVL
                     ENWGSNIIRLFCLSGHYSKPIDYSEKIMMENVTKLRQIETSYYELRLAEGMNKKSYTN
                     EFVNECKEEFDSALNNDFNIPVALTAFYKLIREINSLAADEKITCDVSSIILPELERM
                     MDILGINIVKISDDEKDEINHLIEKRNVYRNEKNFDEADKIREQIAEKNITFIDHKNS
                     TRWVKREKIKAE"
     gene            complement(4576..5787)
                     /locus_tag="QGF01_05140"
     CDS             complement(4576..5787)
                     /locus_tag="QGF01_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HJN20303.1"
                     /translation="MPIKIGLIGKTNTGKTTFFNSSTLSSNEISTYPFTTKKPSKSIG
                     HAITLCVHPEFNVQDNPNNSQCNDGWRYIPIELIDLPGLIKDAWKGKGLGNQFLSIAS
                     QSDALIHIVDASGSIDSTGKITEIGNGDPVSDFADIEEELNMWYQKILEVNRDKLQKI
                     LKTNNDPITALTELYQGIGVKKFHVKDALKATNLEDKDLENFSIQDSKKFSTYLRKIS
                     KPTLIVANKIDVPNADKNFHRLRERYNDMIIVPASADSELTLRRAEQKGLIKYSPGSE
                     QFEILKDDDLNDKQKDALDFIKKDIMGEYMRTGVQFAINVIVFKLLKMNSIYPVADPE
                     KLSDKKGRVLPDLILLKDGATVIDLAKEVHSDLIKGLLYAKDLRYNLRLPINYQLRDR
                     DVICLISAGKK"
     gene            complement(5808..6068)
                     /locus_tag="QGF01_05145"
     CDS             complement(5808..6068)
                     /locus_tag="QGF01_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20304.1"
                     /translation="MDTNDDPVSRAERALYVIQELADSTAEHHPYWVLLYNCSQISKL
                     ILEKWSDDLTEEDLSEIRWMVSELENSCKKLKNKVDQDSRDK"
     gene            complement(6071..6481)
                     /locus_tag="QGF01_05150"
     CDS             complement(6071..6481)
                     /locus_tag="QGF01_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HJN20305.1"
                     /translation="MERDSYTDDEIRNFLLLKKVAVIGISRSESKAAHFVPKYLSENG
                     FDIIPVNPNSNEILSKKCYKEITEVEGDIDIVDVFRPSEDVLPFVRDAIKKNPKVIWL
                     QEGIHNEEAENLAREHGIDVVFNRCMLAEHQRLF"
     gene            complement(6536..7510)
                     /locus_tag="QGF01_05155"
     CDS             complement(6536..7510)
                     /locus_tag="QGF01_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HJN20306.1"
                     /translation="MTSKIRMTKNGITCKIDNLTVNLDPKRLEDNAINFVSHAHMDHL
                     PKHGSGKVITSHETSMIANLRGVNILDSTHTIENFSMLDSGHILGAKGLFFEDIFYTG
                     DISLRDRGFLRGSKIPKCKTLITECTFGKPEFIFPSIEEIVKYVNEIISDAYNRGKPV
                     ILLGYELGKAQLISHLFQHWDPLFYHDSIKRMNDLHIQLGVNLKNTVGHSEAESMGLL
                     EKKPWVMITPNMSSKNAFILDMKKKYDAITIGFTGWAKSSRSFSSTDYTIPLSDHCDF
                     NELMKLVKISGAEIIYTVHGFVDEFADELTKQGFYASSLQESSLDNFT"
     gene            7606..8226
                     /gene="purN"
                     /locus_tag="QGF01_05160"
     CDS             7606..8226
                     /gene="purN"
                     /locus_tag="QGF01_05160"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HJN20307.1"
                     /translation="MLKLAILISGRGSNMKAILDAIKKQNINIKPAVIISNKSSANGL
                     KVAKKFKVKTEIIESKGFQGSRWEYDQKIIKVLKKYQVTPTNGLICLAGFMRIISPEF
                     IKKYKNHILNIHPSILPAFPGLDAQKQAIQHGVRYSGCTVHFVDEGVDSGPIIVQEVI
                     KINANDTEATLSNRILLKEHKAYPKAVKLIADKKIKIMGRKVKILS"
     gene            complement(8223..8540)
                     /locus_tag="QGF01_05165"
     CDS             complement(8223..8540)
                     /locus_tag="QGF01_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJN20308.1"
                     /translation="MKIEYDTSQYIKKIHGSDSYFKTFINKKSLAAGVLLLKPNEEDT
                     QEPHESDEIYYVIKGNGFLQIKKKSYSVKEGNIYFVAKKFPHHFYGNTKDLLVLYFFG
                     GSD"
     gene            8634..>9081
                     /locus_tag="QGF01_05170"
     CDS             8634..>9081
                     /locus_tag="QGF01_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HJN20309.1"
                     /translation="MAKWADYLISEVSYGTNNLITKIKQHHDNGKIISQGEIIDRDTL
                     TNNLGHGAKYMTVYSTLSRFKIGKNVKYFRAFEGHYIRIDNNKVNHDNLGDLPELGES
                     HILQIMNKSDAAPEVAVDAAPEVAVDAAPEVAVDAAPEVSVDAAPEV"
BASE COUNT         3045 a         1446 c         1500 g         3090 t
ORIGIN      
        1 attacagtaa aactgtatta tgtcattttc ataaatatgt ccggcgtttt caagtttctt
       61 gaatacgtat tgaacaaact ccttgttttc aggagaactt gttctataaa agaaatcaaa
      121 ttctatatta aatgctgcaa aatcttcagc atcacgttta ttccaatatt tcacatactc
      181 ttcaggtgtt tttccttctt tctctgattg tattagtata ggtgttccaa agtcatcgga
      241 tgcacaaatg taatacgcct caacaccctt ctgttttaga aatcttgtag ttacatcagc
      301 gggtaaatat gtagatgcga catgtccaag atggatttct ccattcgcat atggcagtgc
      361 acttgtgatg attgcttttg agttcatttg atataggttt tgtaaagttt agttaagaag
      421 ttttaccagc tactcatgta tttgatctgt tcttttgtta acgtgtcaat cttcacattc
      481 atagctttca aagcgtcaat tgctacctgt ttatctatct cacttggaac tgtcacaacc
      541 ttttttggca tttttttatg atttttgaga atgtaaagaa ttgaaagtag ttgatttgaa
      601 aaagactgtg ccatcacttc tggcggatga ccttctgcgg ctgtcaagtt agctattcta
      661 ccctcaccga taagatagac ttttttgcca tttttcagta cacattcatc aagatatgga
      721 cgtacttgtc taacagattt tgactgcttc aaaagaaact tggaatccac ttcaacgtca
      781 aagtgaccaa cgtttccaag cactgcgcca tccttcatct ttaagatatg ttcctttctg
      841 atgatactag tcattccagt tgttgtaatg aaaatctcac caatttttgc agcctgtgcc
      901 attggcatta catcaaagcc atccatatgc gcttctagcg cacgtatagg attcacctct
      961 gttactatga ccttagcgtt cattccacga caatttgctg caacaccttt tccgacccag
     1021 ccatatcctg ccaccacgac tcgtttagat gcaaataaca aattcattgc tcgtaagtaa
     1081 ccatctattg tactttgacc agtaccgtat ttgttatcaa acatatgttt tgtatatgca
     1141 tcattaactg aaattacagg atatcgaagt ttaccttgtt tttctacagc tctaattcgg
     1201 tttacacctg cagtagtttc ttctgtagcc ccaaaaattt tcaacgagtt gtatttttta
     1261 tcaaaatgag cttttacatt catatcagaa ccatcatcag tcaaaatctg tggtttatgg
     1321 tttagaactt gttgtataca ccaatcatat tccttaggac tttgaccagc ccaacaatat
     1381 gtatgaattc cctgtgatgc aagaaaagct gctatgtcat cctgagtagt aagtggattt
     1441 ccaccacaac atgcaaccgt agctcccagt tcttttgcac ccataagtaa aactgatgtt
     1501 tcttttgtta tgtgcaaaca aaatcccaat gtgattcctt tgagaggttt tgattttttt
     1561 aatctcatta tagtattatc taagatctgc atatgagatc tcgcccattc ataggaaatc
     1621 ttacccttct ttgctaattt tttatctttt actttactca acgtaaattt ggattttaac
     1681 taggtataaa attctatcac aacctacaag ctttttacta tgtcaaaatg actagaatta
     1741 gtatgacatg gaaaaaaata actgaaaaaa gcacaattgt aagtggtaaa ggtatggaat
     1801 ttgaaattga tgggaagaaa attgctgttt ttaatcaaaa tgggtatcat gcgttagatg
     1861 ctctatgtgt acatcaagat ggttctattg cacctggtaa acttgatggt gatattgttg
     1921 aatgcccatt acatttttgg cattacaata ttaaaactgg tgagctgaag gattttctga
     1981 aagatatcaa acttcaaaca tacaagactg atgtgagaga tgacggtgtc tatattgatc
     2041 tgtaaaaaat tatagtgagt caataaataa tgattttaat acatctaatt tttcgtagaa
     2101 gatttggatt atgttattga aattatcctg aactgctgga tcattctgaa gattttctgc
     2161 atcatctaat gcagcatgtg ccaaggatgg aatgtcgatt tgctcaattg tgtcaggaaa
     2221 taacaacatt ccaccgccaa ttaaaatagc accaactata atcaattttc ctttcatttg
     2281 ttcatgattc caaaaaaatg ataaaaaatc ggaaataaaa aaattttgag taaagatgca
     2341 aaactgtctt gcaaagataa caatttatgc ctatatcgag caaagattgt gtgaatcatg
     2401 aaattctacg tgaaattatt actcaagatt atgtaaaaat tcaaaatgat attgaaaaat
     2461 ttctaaaaga tagtgttttg gagaataaag ctgatggtgt tatttttggt ctgagtggtg
     2521 ggatagactc tgcgataatt gcatatttat ctgcgaaaat atttggtaaa aaagcacttg
     2581 ctttagtaat gccagactct acggtatcac catcaagtga aacaggtgat gcgttaaaaa
     2641 taattggtga attaggtatt gattacaaac ttatcgatat tgatgtaatc cataagatat
     2701 attcaaatta tttagaacct gagaaacgag cattaggtaa tctgcgagca agaataagag
     2761 cgaatatcat atactattat gcaaatttaa aaaatttatt agttttagga acaagtgata
     2821 aaagcgaata ctccataggt tactttacaa aatttggtga tgggtgtgct gatctactac
     2881 caatttctaa cctttacaaa acacagatac gagaatttgc aaaaatcctt ggaatcccag
     2941 ataacataat cacaaaaaaa agtagtccta atctatggaa ggaccatgat gcagaagagg
     3001 aaattggtat cagttacgaa gaaattgatt caatattata ttgtattgaa aaaaaaataa
     3061 gtgttgatga ggttgcaaaa atgacagaaa taaaaaaaga gtttgtggaa aagattatcc
     3121 aaatgcatga gaacagtaag cataaaagat taccacctgt aggtatcaat gaaggatgat
     3181 agtataatgc aaatatttga taccctaaac aatacaaaaa cagaattaaa atttttggat
     3241 aaagttacaa tatatttatg tggagttaca gtttatgatg atgcacatat tggtcatgca
     3301 cgtacaataa ttgtttttga tgtattacgt agatttttag aatcacaaaa aaatattgtt
     3361 gaaattgttc aaaattttac ggatgtagat gataaaataa tagcacgtgc aaatgaaaag
     3421 aaagtgtcca ctgtcgaact cacttctaaa tacactgaaa attactttcg tgattttgat
     3481 aaactgaatg tgaaaagagc tactcattat ccaaaggcaa cagaacatat tgaagatatg
     3541 ttgaatttaa tttctcaact tattgataaa aaatgtgcgt atataactaa aaacggtgtt
     3601 tatttttcag tttctaaatt tcgtgattat ggaaaattat ccaaaaagaa tatcaaagaa
     3661 ttgatttcgg gttcacgtgt aatggtagat gaggaaaaaa atgatccgct tgattttgca
     3721 ttatggaaat tttctgatac tcaaccaatt tggaatagtc catggggaaa aggacgacct
     3781 ggatggcata tagaatgttc tgcaatgagt agaaaatatc ttggtgatgg ttttgatata
     3841 catggtggtg gtagagatct tatttttcca catcatgaaa atgaaattgc gcaatcagaa
     3901 tcatcaacat caaatcagtt tgcaaaaatt tggatgcatg ttggtatgat aaccataaac
     3961 ggtgaaaaaa tgtctaaatc aattgggaat gtaaaatcag taagtcacgt gctggaaaac
     4021 tggggatcaa acattattag attattttgc ctttcaggcc attactcaaa accgattgat
     4081 tactctgaaa aaattatgat ggaaaatgtt acaaaactta ggcagattga gacatcatat
     4141 tatgaattaa gactggctga aggcatgaat aaaaaatcat atacaaatga gtttgtaaat
     4201 gaatgtaaag aggaatttga ttctgcatta aacaatgact ttaacatacc agttgcacta
     4261 actgctttct ataaattgat aagggaaatc aattctttgg ctgctgatga aaaaattact
     4321 tgtgacgtat caagtataat tttgcctgaa cttgaaagaa tgatggacat acttggtatc
     4381 aatattgtaa aaatctcaga tgacgaaaaa gatgaaatca atcatcttat agagaaaaga
     4441 aatgtgtata gaaatgaaaa aaactttgac gaagctgata aaattcgtga gcagatagca
     4501 gaaaaaaata ttacctttat tgatcataaa aattctactc gatgggtaaa acgtgagaaa
     4561 attaaggctg aataattatt tttttcctgc actgataagg cagattacat ccctatcacg
     4621 taattggtag tttattggta acctcaaatt atatctaaga tcttttgcat acaatagacc
     4681 ttttatcaag tctgaatgga cttcttttgc taaatcaata actgttgcac cgtcttttaa
     4741 tagaatgaga tcaggtaaaa cacgtccttt tttatcagaa agtttttcag ggtctgcaac
     4801 aggataaata gaattcattt tcagtagttt aaacacgatt acgtttatcg caaactgtac
     4861 acctgtacgc atatactcac ccataatatc ttttttaatg aaatctaatg catctttttg
     4921 tttatcgttg agatcatcat ctttcaatat ttcaaattgc tctgaccccg gtgaatattt
     4981 tataagccct ttttgctccg ctcgccttaa agtaagctca ctatctgcac ttgcaggtac
     5041 aataatcata tcattatatc tttcacgaag acgatgaaaa tttttatcag cgtttggaac
     5101 gtcgattttg tttgcaacaa ttagtgtagg ttttgaaatt tttctaagat acgttgaaaa
     5161 ttttttgcta tcttggatgc taaaattttc cagatcttta tcttcaagat tagtggcctt
     5221 taatgcatct ttaacatgga attttttaac acctatgcct tgataaagtt cagtcaaagc
     5281 agttattgga tcattattgg ttttgagaat tttttgtaac ttgtcacgat tcacttctag
     5341 aatcttttga taccacatat tcaattcttc ttcaatatcg gcaaaatctg aaaccggatc
     5401 accattacct atttcagtaa tttttccagt tgagtcaata cttccagatg catcaactat
     5461 gtgtatcaat gcatctgact gtgaagcgat agaaagaaat tgatttccca aacccttacc
     5521 tttccaggca tcttttatca atccaggaag atcaatcaat tcgataggaa tgtatctcca
     5581 accatcatta cattgagaat tatttgggtt gtcctgcaca ttgaattctg gatgaacaca
     5641 aagtgttatg gcatgtccaa tagatttgga tggtttttta gtagtaaaag gatatgtcga
     5701 tatttcatta gatgaaagtg tcgaagagtt aaagaaagta gtttttcctg tgttcgtttt
     5761 cccgataaga cctattttaa ttggcatgat taaaaaatga tttaaaatta tttatctctg
     5821 ctgtcttgat ctactttatt tttgagtttt ttacacgagt tttctaattc ggaaaccatc
     5881 caacgtattt cacttaaatc ttcttcagtt agatcatcac tccatttttc taaaattaat
     5941 ttagaaatct gggagcagtt atacaataac acccaatatg gatgatgttc agctgttgaa
     6001 tctgctaatt cttgaataac atataatgct ctttctgcac gtgatactgg atcatcatta
     6061 gtgtccatga ttaaaaaagg cgttgatgct ctgctagcat acatctgtta aaaactacat
     6121 caattccgtg ttctctagca agattttcag cttcctcatt atggataccc tcctgtagcc
     6181 atattacctt agggtttttt ttaattgcat ctcttacaaa tggcaagaca tcctcagatg
     6241 gtctaaacac atcaactatg tcaatgtcac cttctacttc ggtaatttct ttgtaacatt
     6301 ttttagataa aatttcatta ctgttaggat tgactgggat tatatcaaaa ccattttctg
     6361 acaaatattt tggaacaaaa tgtgctgctt tagattcgct tcttgatata ccaataactg
     6421 caactttctt taataacaga aaatttctga tttcatcatc tgtatacgaa tctctttcca
     6481 tgttgatcat ctgcaatgaa tttttattat tcttaagtgt gaggtgattc tatagttatg
     6541 taaaattatc aagtgatgat tcctgtaatg aactagcata aaatccttgt ttggttaatt
     6601 catctgcaaa ttcatcgaca aaaccatgta cagtataaat tatttctgct cctgaaattt
     6661 ttactaattt cattaattca ttaaaatcac aatgatcact caatgggatt gtataatctg
     6721 tactagaaaa agatcttgaa gatttagccc atccagtgaa ccctatagta attgcatcat
     6781 attttttttt catatctaaa ataaatgcat tctttgatga catgttaggt gtgatcatga
     6841 cccaaggttt tttttcaagg agtcccatag attcagcctc agaatgacct actgtatttt
     6901 ttaaattaac gcctaattgt atatgtaaat cattcattcg ttttattgaa tcatgataaa
     6961 ataatggatc ccaatgttgg aatagatgag aaattagttg cgcctttcct aattcatatc
     7021 ctaaaagtat gacaggtttc ccacggttat atgcatcaga aattatctca tttacatatt
     7081 ttacaatttc ctctatgcta ggaaaaatga attccggttt accgaatgtg cattctgtga
     7141 tgagagtttt acattttgga attttagatc cacgtaaaaa tcccctatca cgtaaagaaa
     7201 tatctcccgt gtaaaaaata tcctcaaaaa ataatccttt tgcacctaaa atatgaccag
     7261 aatccagcat agaaaaattt tctatggtgt gggtagagtc taaaatattc acaccgcgta
     7321 aattagctat catagatgtt tcgtgtgatg tgataacctt accactgcca tgctttggta
     7381 aatgatccat gtgtgcatgt gatacaaaat tgatagcatt atcttcgagt ctttttggat
     7441 ctaaattgac agttagatta tctattttac atgttatacc gttctttgtc attctaattt
     7501 tacttgtcaa atgctttatc aaaagataga tttgatataa attactagtt tgattggtta
     7561 cgagcttacc atatctttat ttttgtgatt gtagaagaat atttgttgct aaaacttgcc
     7621 attctgattt caggtagggg aagtaacatg aaagctattc ttgatgcaat aaaaaaacaa
     7681 aatatcaaca taaaaccagc agtaattatt tccaacaaat cttctgcaaa tggactaaaa
     7741 gttgctaaaa aatttaaggt caaaacagaa ataatagaaa gtaaagggtt tcagggaagt
     7801 aggtgggagt atgatcaaaa gatcattaaa gttttgaaaa aatatcaagt tacacctaca
     7861 aatggtttga tatgtcttgc aggatttatg agaatcatca gtcctgaatt tattaaaaaa
     7921 tataaaaatc atattttaaa tatacatccg tccatcttac ctgcattccc aggccttgat
     7981 gcacaaaagc aggcgattca acatggtgtg aggtattctg gctgcactgt tcattttgta
     8041 gatgaaggtg tagatagtgg tccaattatt gttcaggaag ttatcaaaat caatgctaat
     8101 gatacggaag caacgctctc aaatcgaata ttactaaaag agcataaagc atatcctaaa
     8161 gctgtcaaac taatagcaga taaaaaaata aagattatgg gcaggaaagt gaagatactt
     8221 tcttaatcag acccaccaaa aaaatacaaa accaaaagat cttttgtatt tccataaaag
     8281 tgatgtggga acttttttgc aacaaaatag atatttccct ctttaactga ataagatttt
     8341 tttttgattt gtaaaaatcc atttcctttt atcacataat atatctcgtc agattcgtgt
     8401 ggttcttgtg tatcttcctc gtttggtttt agtaataaaa caccagctgc taacgatttt
     8461 ttatttatga aagttttaaa ataggaatcg ctaccgtgta ttttcttaat atattgagat
     8521 gtgtcatatt ctattttcat aaaaatttaa cctttcttca tcgtttaaag attagtttcg
     8581 atcattgaga aaaaacattg cttatttagg gggtgtttta tagtattttt taaatggcaa
     8641 aatgggctga ctatctaata tctgaagtgt catatggaac aaataatctt atcaccaaaa
     8701 taaaacagca tcatgataat ggtaagataa tcagtcaggg agagattata gatcgtgata
     8761 cacttacgaa taatttagga cacggtgcta agtatatgac ggtttacagt actttaagcc
     8821 gttttaaaat tggaaaaaat gtaaaatact ttagagcatt tgagggtcat tacattagaa
     8881 tagataacaa taaggttaat catgataatt tgggagactt gcctgaactt ggtgaatccc
     8941 atatattaca aattatgaac aaatctgatg cggcaccaga agtagctgtt gatgcagcac
     9001 cagaagtagc tgttgatgca gcaccagaag tagctgttga tgcagcacca gaagtatctg
     9061 ttgatgcagc accagaagta t
//
LOCUS       DASCPJ010000107         5303 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001696517, whole genome shotgun sequence.
ACCESSION   DASCPJ010000107 DASCPJ010000000
VERSION     DASCPJ010000107.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001696517"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="QGF01_05175"
     CDS             <1..575
                     /locus_tag="QGF01_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20310.1"
                     /translation="AYVKNCLVESQVCITKAISGLGTDPYAGCWLNSSSYFIADAICG
                     IGLQQPAPTHMLKQLRELKKNKINEFISILTELIGIERATPSLLSRMFKSTIGFSDMI
                     EKNQHSMLISKKYNYMINHSLLSDCYFYLGYINRNNFFKIQDLHKKPEFIHILKTAFD
                     LETDASKIESQANKLHETIDTIIKLVHKSN"
     gene            651..794
                     /locus_tag="QGF01_05180"
     CDS             651..794
                     /locus_tag="QGF01_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20311.1"
                     /translation="MTDQACGCEADSGDPDYACDCAMQGHCICSHDCKCNTDVCKEAV
                     SQL"
     gene            complement(844..1020)
                     /locus_tag="QGF01_05185"
     CDS             complement(844..1020)
                     /locus_tag="QGF01_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20312.1"
                     /translation="MCECTKIHFYEVEFKLDGMVIVPTHKNCGDKLNEKQADTFQEQL
                     VKSWGFEEEEAAED"
     gene            1095..2612
                     /locus_tag="QGF01_05190"
     CDS             1095..2612
                     /locus_tag="QGF01_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HJN20313.1"
                     /translation="MHYEKTTIFFLSIILIGGTVFPATFAQDAPPPQAGTGATSSGGV
                     DHAGSWYPGENLKINDYFKYELCHAEYKDCTEFWFTMWVEKKVVENNEEKFRIQVLVE
                     DGNRVVKGHMDVGMLAPEPTGGSDNISSYRSVYKSTIAWLTAFATAPTDLAGKGPKEF
                     SKPSWGKIANIGGEQVSPIGMQTIHTPSGEHETVVIGWKTGGKSSHIWVVDEFPFPVK
                     ASTWVQVTEGIPPQEYKFSLFDYKENVSTNPFINVVDTEQQKADANCPTEYVKEKITT
                     NTNTNSMQVTIFYGPETPKIGCDLEFLIEFKKSYSSDIWENQVHYDILKVDSELVPIA
                     SAADDEGRDKFFSTSGQVHRYWLMTGDPGLQKFALLVKGTGPEHITPNPEFFGYVIFD
                     IDILSAGKGSGSLQTITPTITETEIIIPNWIKNNAGWWADGIIPDSAFVSGIQWLISN
                     DIMNIPPTEQGEGSDNMIPAWIKNNAGWWANGEIPDSAFVSGLQWLISNGIMNIS"
     gene            2668..3024
                     /locus_tag="QGF01_05195"
     CDS             2668..3024
                     /locus_tag="QGF01_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20314.1"
                     /translation="MTGLFSYPKRNLRKLIKNGDFKEAIELGNNLEEKYSQDHDFLFI
                     MGSMFYILEDPKMTLHYIDRVLEISEYDVEALSLKLRVHQHLKENDIAIECCKKILSV
                     NSDNYEVKDVLTELEG"
     gene            complement(3025..3585)
                     /locus_tag="QGF01_05200"
     CDS             complement(3025..3585)
                     /locus_tag="QGF01_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20315.1"
                     /translation="MIFTIITNDLQKELKSNLPQIMVLLKKSPAIAYKTIGDIGKEVG
                     RKYNIELLVNFPHKGKIENFDMYGKQDLSFIIDMKKRSFPIERSKIKEKAKEVFGNIE
                     VEDAYMHDDSEGVKVFLGPINESGRKEDRIDILPHSLHIWYEFTDKVTEFCDWLLENV
                     YSVKEIDHKGETRYEKFRAEKKGENV"
     gene            3657..4298
                     /locus_tag="QGF01_05205"
     CDS             3657..4298
                     /locus_tag="QGF01_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20316.1"
                     /translation="MSLPKEGDFVPLDDSERAPNVDSYRITCIDFIKDISKDYLAWVD
                     RYKLPEEEDRKRRTEISAIIERTNGWIAKIPQSFKAVDVVMNDGTQKMAEATAGKPFQ
                     IGVFVDKKSGYTLAKPGIINVNIKAAGREKNKTKLAFHTKEERFRIESTGKAFFDESN
                     ITDREYDLLDINLNLNATECQQRDVISFTVIVSEMQNGMEIDRRGVSTVIHIV"
     gene            complement(4307..4735)
                     /locus_tag="QGF01_05210"
     CDS             complement(4307..4735)
                     /locus_tag="QGF01_05210"
                     /inference="COORDINATES: protein motif:HMM:NF021378.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HJN20317.1"
                     /translation="MDNKSEKYLIKYLKSLPDNQIKQFYNGVEWTPYPVLVIKEFQRR
                     FNPNDDEFATKLLESVNEAKTKGQKIGRLAKLRGLKLSKKVSTQAKKTVSKKVTKAKR
                     MIRSSQDNVELIKKLGELKKAGIITNKEFQTKKKQLLDKI"
     gene            4790..>5303
                     /locus_tag="QGF01_05215"
     CDS             4790..>5303
                     /locus_tag="QGF01_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN20318.1"
                     /translation="MTREAILYEKRPNDKVLCTACARYCEIGKGQIGLCGIRGNENGK
                     LDLYVYGKVIAGHVDPIEKKPVIHYNPGSSIFSIATTGCNWLCKYCQNSDISQRRKIE
                     GVDMTPEQVVNTAVDHGADGIAYTYNEPSIFMEFARDCGVTARKKGLFNIFVSNGYDT
                     PESVKMMDEFL"
BASE COUNT         1739 a          870 c          964 g         1730 t
ORIGIN      
        1 atgcatatgt aaaaaattgt ttggtagaat cacaagtatg cattacgaaa gcaataagcg
       61 gattaggcac agatccatat gcaggatgct ggcttaactc atcatcatat tttattgctg
      121 atgcaatatg tggaattggt cttcaacaac ctgcccccac tcacatgttg aaacaattac
      181 gtgaactgaa aaaaaataaa attaacgaat ttatatcaat tttgactgaa ttaattggta
      241 tagaacgtgc aacgccatcc cttctttcaa gaatgttcaa gtccactatt ggtttttccg
      301 atatgataga aaaaaatcaa cattctatgc ttatctctaa aaaatataat tatatgataa
      361 accattcatt attatctgat tgttattttt atctgggtta tatcaacaga aataactttt
      421 tcaaaattca ggatcttcat aagaaacctg agtttattca tattttgaaa actgcatttg
      481 atttagagac tgatgccagc aagattgagt cacaagccaa taaattacat gaaaccatcg
      541 acacgataat aaaactagta cacaaatcaa attgatattt tatatgtctg gatataacga
      601 tgtatgattt ttttataaag tcttaaaaac atgtccagac tcattatctt atgacagatc
      661 aagcatgtgg ctgtgaagca gattcaggcg atccagatta tgcatgtgac tgtgcaatgc
      721 aaggtcattg tatatgcagt catgattgca agtgtaatac tgatgtatgt aaagaagcag
      781 tttctcaact ttaaaacatc atcccttatt tttatacaaa tattttgata ttatctagtc
      841 ttactaatct tctgccgctt cttcctcttc aaatccccat gattttacaa gctgttcctg
      901 aaatgtatca gcctgttttt cattcagctt atcaccgcag tttttgtgtg tcggaactat
      961 gaccatacca tccagtttga attcaacctc ataaaagtgg atttttgtac attcacacat
     1021 atacacaaag ttatctttaa gccctatatt tgcttatttg aaaattttaa tacccaatta
     1081 atacgattga gtacatgcat tacgaaaaaa ccactatatt ttttctgtca atcattttga
     1141 ttggaggtac tgtattccca gcaacttttg ctcaagatgc tccaccgcct caagctggta
     1201 ctggagcaac ttcaagtggt ggagtggatc atgcaggatc ttggtaccca ggagaaaatt
     1261 taaagataaa tgattatttc aaatacgaat tatgtcatgc agagtataaa gactgtacag
     1321 aattttggtt tacaatgtgg gtagaaaaga aagttgtaga aaataatgaa gaaaaattcc
     1381 gaatacaagt attggttgaa gatggaaata gggttgtaaa aggacatatg gatgttggca
     1441 tgcttgcacc tgaaccaact ggtggcagtg ataacatatc ttcatatcgt tcagtataca
     1501 aatctactat agcatggctt actgcgtttg ctactgcacc aactgattta gcaggcaagg
     1561 gaccaaaaga attcagtaaa ccatcgtggg gtaagattgc gaatattggt ggagagcaag
     1621 tatcgccaat tggtatgcag actatacata ccccatcagg tgaacatgaa actgttgtta
     1681 taggttggaa gactggtggt aagtcaagtc atatttgggt agtagatgag tttccatttc
     1741 ctgtaaaggc tagtacatgg gttcaagtaa cggaaggaat accaccacaa gaatacaaat
     1801 tttctctatt tgattacaaa gaaaatgttt ctacaaaccc attcataaat gtggtagata
     1861 cagagcagca aaaggctgac gcaaactgtc ctactgaata tgttaaagaa aaaataacta
     1921 ctaatacgaa tacaaattca atgcaagtaa ccatatttta tggtccagaa acacccaaga
     1981 ttggatgtga tcttgaattc cttatagaat ttaagaagag ttattctagt gatatatggg
     2041 aaaatcaagt tcattatgac atattgaaag tggattcgga attagttcca atcgcttctg
     2101 cggctgatga tgagggacgt gataagttct ttagtacgtc aggacaggtt catcgatatt
     2161 ggttaatgac tggtgaccca ggtttacaaa aatttgcttt gttggtgaaa gggactggac
     2221 cagaacacat cactccaaat cctgaattct ttggatatgt tatttttgat attgatatat
     2281 taagtgctgg taaaggctct ggcagtttac aaactattac tcccacaata acagaaaccg
     2341 aaattattat tccaaattgg attaaaaaca atgcgggatg gtgggcagat ggaattattc
     2401 ctgactctgc atttgtttca ggtattcaat ggctcatatc taatgatatt atgaatatac
     2461 caccaaccga acagggggag ggttctgaca acatgattcc ggcatggata aagaataatg
     2521 cgggatggtg ggctaatgga gaaattcccg actctgcatt tgtttcaggt cttcaatggc
     2581 tcatatcaaa cggaataatg aatatttctt gatcatatta tattgctatc acagtttggt
     2641 tagaatgcat acaaaaggag aaaaattttg actggtctat tttcttatcc aaaaagaaat
     2701 cttagaaaac taatcaaaaa tggtgatttt aaagaagcaa tagaacttgg gaataattta
     2761 gaagaaaaat attctcaaga tcatgatttt ctttttatca tgggtagtat gttttacata
     2821 cttgaagatc caaaaatgac actccattac attgatcgag ttctagaaat tagtgaatat
     2881 gatgtagaag cgctttctct taaattaaga gtgcatcaac atttgaaaga aaatgacata
     2941 gctattgaat gttgtaaaaa aatcctatct gtgaattcag ataattatga agtgaaagac
     3001 gttcttactg agttagaagg atagttacac attttcacct ttcttctctg ctctaaattt
     3061 ctcgtatctt gtttctcctt tgtggtctat ttctttcacc gaatacacat tttccaaaag
     3121 ccaatcacaa aattctgtga ctttatctgt aaattcatac cagatgtgta aagagtgtgg
     3181 taaaatatca atacgatcct cctttctccc actttcattt atgggaccaa gaaagacttt
     3241 gacaccctca ctatcatcat gcatgtatgc atcttccact tcaatatttc caaaaacctc
     3301 ctttgctttt tcctttattt tgctacgctc aatagggaaa cttctttttt tcatgtctat
     3361 aataaaactc aaatcctgtt ttccatacat gtcaaaattt tctatttttc ctttgtgtgg
     3421 aaaattcact aataactcaa tattgtattt tctcccaact tcttttccta tatcacctat
     3481 tgttttgtat gcaatcgcag ggcttttttt taataaaacc ataatttgtg gcaagttcga
     3541 ttttagttct ttttgtaaat catttgtaat tattgtgaag atcatacaga aattatgtat
     3601 tctttgaata tattgattaa gattgggttt tttaataata agattatcct gattttatgt
     3661 cattaccaaa agaaggtgat tttgttccac ttgatgattc ggaaagagcc ccaaatgtag
     3721 atagttaccg aatcacttgc atagatttta tcaaggatat atcgaaggat tatcttgcat
     3781 gggtagatcg ttacaagcta ccagaggaag aagacagaaa acgacgaaca gagatatctg
     3841 ctattattga acgaactaat ggctggattg ctaaaattcc acaatctttc aaggctgtag
     3901 atgtagtcat gaatgatggg acacaaaaaa tggcagaagc gacagccgga aaaccatttc
     3961 agattggtgt ttttgtagat aaaaaatctg gctacacact agcaaaacct ggaattatta
     4021 atgtgaacat aaaggctgca ggtagggaga agaataagac aaagcttgca tttcacacta
     4081 aagaggaaag gttcagaata gaatcaaccg gcaaagcatt ttttgatgaa tcaaatatta
     4141 cagatagaga gtatgatctt ttggacatta atctgaatct aaatgctaca gaatgtcaac
     4201 aacgtgatgt catatcattt acagttattg tgagtgaaat gcaaaatgga atggagattg
     4261 atcgacgtgg agtgagtacc gtcattcata ttgtttagtt taacaattag attttatcaa
     4321 gaagttgttt cttttttgtc tgaaattctt tattggtaat aataccagcc tttttcaact
     4381 cgccaagttt tttgattaat tcaacattat cttgagatga tctaatcatt cgttttgctt
     4441 ttgtaacctt ttttgaaact gtttttttag cttgtgtact gacctttttg cttaacttta
     4501 ggcctcttaa ttttgctaat ctgccaattt tttgtccctt ggttttagcc tcattcactg
     4561 attctagaag ttttgtggca aattcatcat catttggatt gaagcgtcgc tgaaattctt
     4621 ttatgacaag aacaggataa ggcgtccatt ctacaccatt ataaaattgc ttgatctgat
     4681 tatctggaag tgacttcaga tatttgataa gatatttctc agatttatta tccatagata
     4741 tatttctcat tcaagttatt aataatgatc taataatcat actagtgata tgacaagaga
     4801 agctatcctt tacgagaaaa gacctaatga caaggttttg tgtactgcat gcgctaggta
     4861 ttgtgagata ggaaaaggcc agattggtct ttgtggtatc agaggaaatg aaaatggtaa
     4921 actcgatctt tacgtttatg gaaaagttat cgcaggccat gtcgatccaa tagaaaagaa
     4981 accggtgatt cattataatc cgggaagcag tattttctca atcgcaacca ctgggtgcaa
     5041 ctggctatgc aagtattgcc aaaactctga catcagtcag agacgcaaaa tagagggcgt
     5101 tgatatgact ccagagcagg tggtaaacac tgctgttgat catggagcag atggaatagc
     5161 atacacatac aacgaacctt caatattcat ggagtttgct cgtgattgcg gtgtgactgc
     5221 aagaaagaag ggactcttca atatttttgt ctccaacgga tatgatacac cagaatcagt
     5281 aaagatgatg gacgaatttt tgg
//
LOCUS       DASCPJ010000108        10048 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001738918, whole genome shotgun sequence.
ACCESSION   DASCPJ010000108 DASCPJ010000000
VERSION     DASCPJ010000108.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001738918"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="QGF01_05220"
     CDS             complement(<1..357)
                     /locus_tag="QGF01_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HJN20319.1"
                     /translation="MSFLDLHMTNSPLIINPNDNDDSKVTIFGIPFDSTHSYKPGCRF
                     GPDAIRDAFNNIEIFQPEFGVDLETVNINDLGNTKHTVVATEMLRMVENITSELAKQG
                     KQIIILGGEHLITLGSF"
     gene            complement(362..2212)
                     /locus_tag="QGF01_05225"
     CDS             complement(362..2212)
                     /locus_tag="QGF01_05225"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HJN20320.1"
                     /translation="MRILQLHCDSIEYTPTKKEIKSAEEIEPKSTRIEEVVVCFIAVE
                     KDDDSNIAKNAMIEIQETMKKVGCNKLLLYPYAHLSSDLASPGTGLKILKEMQEICTG
                     IEVTHAPFGWTKAFSIQVKGHPLAESSKVFSKDSIKEKTSTALESESKIKSYWYIMTP
                     EGKMEEIEKFNFSNHKQLEILAKYESAKTRSSDEAPPHVNLMKKLAIADYEPASDSGN
                     MRFYPNGRLIKSQIEQYVTDKIRNYGGIEVETPIMYDSYHPSMESYFNRFPARQYSID
                     SEGKHLFLRFAACFGQFLMTKDFQLSYKNLPYKLYELTRYSFRREQSGELVGLRRLRA
                     FTMPDCHAFCKDMPQAIEEFRKRFDLSREVLSGLGIEENEYEMAIRFTDEFYNQNKEL
                     IKQMVSKIGKPVLVEMWKERFFYFVLKWEFNFIDNLGKASALSTDQIDVENGKRYGIT
                     YTNENNEQKHPIILHNSPSGAVERVIYALLEKFAKQIKDGKRPQFPLWLAPIQVRLIP
                     LKDEYLEFTTHLADKFIEQNIRVDIDDRNDTIGKRIRDAEKEWISYVLVIGEKEVNSP
                     KLSIRDRTTGDVREVSIEDFIKEITDATSDKPTSRLNSTLLLSQRPQIMV"
     gene            complement(2258..2422)
                     /locus_tag="QGF01_05230"
     CDS             complement(2258..2422)
                     /locus_tag="QGF01_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20321.1"
                     /translation="MTEWKKGISDKEIEKTDKMIDDLIDMIDDKRDDLSLDFITNLTK
                     YFKNLLKSEK"
     gene            complement(3109..3330)
                     /locus_tag="QGF01_05235"
     CDS             complement(3109..3330)
                     /locus_tag="QGF01_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20322.1"
                     /translation="MDFKEKSDFVLGMTENIKEWVINQPKKRCGLIEGHDNYWEGKID
                     AIKKVLDETLSTPRDSERALNEIKKILES"
     gene            complement(3355..3540)
                     /locus_tag="QGF01_05240"
     CDS             complement(3355..3540)
                     /locus_tag="QGF01_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20323.1"
                     /translation="MTEPEQDKKYEGSHFTPDIICPFCHKNIKEHVKDCIGKRFCVTA
                     KDRSENSERLRKLYKNR"
     gene            complement(3550..3681)
                     /locus_tag="QGF01_05245"
     CDS             complement(3550..3681)
                     /locus_tag="QGF01_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20324.1"
                     /translation="MNDKEKIEKALEKIDAFIKMKSKYNEIAYSMRDLKEIKEILEE"
     gene            complement(3741..3992)
                     /locus_tag="QGF01_05250"
     CDS             complement(3741..3992)
                     /locus_tag="QGF01_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20325.1"
                     /translation="MSDTEHNLRIEKIAQMITSDHVSIDEQDQSKLKKYYDFAVQNYS
                     LESSMAKELVHEAFLYLKLKDGGDIDPLTKGDEFGAGFS"
     gene            complement(4031..4180)
                     /locus_tag="QGF01_05255"
     CDS             complement(4031..4180)
                     /locus_tag="QGF01_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20326.1"
                     /translation="MSSKFYHRKLSEYDEKPTCSLCDVKSLYYCNMNDEYYCVNHILD
                     HDENE"
     gene            complement(4182..4514)
                     /locus_tag="QGF01_05260"
     CDS             complement(4182..4514)
                     /locus_tag="QGF01_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20327.1"
                     /translation="MLKYIDIPNPKRLEREPDKIISILTNGNSIENGYEIRNIFLRLY
                     IEKIDKELGPYSVITSLVETTEDSVEMIYDEGYFDTKEFENTSKFLINNLGISSVILR
                     SLVFLDNR"
     gene            complement(4562..4777)
                     /locus_tag="QGF01_05265"
     CDS             complement(4562..4777)
                     /locus_tag="QGF01_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20328.1"
                     /translation="MGIISKGAKCNVADCKNDGIRSLNTSKVENAGLRVSATGKKTVL
                     CKTHYKEWKKETKEDRSLERSRYDKFS"
     gene            5055..5369
                     /locus_tag="QGF01_05270"
     CDS             5055..5369
                     /locus_tag="QGF01_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HJN20329.1"
                     /translation="MGGSKNISPAQKEKKQQRSSDTKKGKKDSKGDKNEKAEISVILN
                     ETDAEKIIKNAKIITLYDFARQTGVKISTANEFLQKSLKNGAIKKIGGFSGHHIYQPI
                     SE"
     gene            complement(5351..5737)
                     /locus_tag="QGF01_05275"
     CDS             complement(5351..5737)
                     /locus_tag="QGF01_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20330.1"
                     /translation="MIQNTVSSINLFLDINGKSKISCQLKRHLSPKTVGLITRSTPLQ
                     GNAHRNSESSVYMETAIDSGIERKRTDFKMGDIAFFPIEGSIFFYIKDVYGGKPMTPI
                     GRILSDIEKLKQIKPGDLLSLYSEIG"
     gene            5787..6287
                     /locus_tag="QGF01_05280"
     CDS             5787..6287
                     /locus_tag="QGF01_05280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HJN20331.1"
                     /translation="MPKSDNDVDEVKLEDSDVDKKTDDVKSSFENDSVDNAITAYRKL
                     FDVKDVEITEKQQAEIDQVCEVMRKREIIYKETEHEPEEITSNDNKIITGPPMLTRFE
                     KARIMGARALQLSLGAPPFISVPKDTSTSLEIAMEELNERLIPIVIRRVLPNGDYQNI
                     PINHFK"
     gene            6339..6641
                     /locus_tag="QGF01_05285"
     CDS             6339..6641
                     /locus_tag="QGF01_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014029.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20332.1"
                     /translation="MTTDMESNGSDSFTDEIFLVDSDEPIMNIAFDALEMIRKNKKII
                     VKGKGEMCSKAVAVSNILTERMLKETAKFGKIDVDSEMLDDGQLISTIKLAIVMTD"
     gene            complement(6642..7058)
                     /locus_tag="QGF01_05290"
     CDS             complement(6642..7058)
                     /locus_tag="QGF01_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJN20333.1"
                     /translation="MLLPAEIESKTLIPALRAILAKKLAANHDIREDEISRMLGVTQA
                     AVSNYIRGIRGNPELIEKLLAEEQIVKMIADITDRLAAEKDYTPATLSKFIGLCNYIK
                     TSLLICDIHHNLESNIDDKICKECENMLLKGPGSVY"
     gene            7168..8118
                     /locus_tag="QGF01_05295"
     CDS             7168..8118
                     /locus_tag="QGF01_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HJN20334.1"
                     /translation="MNEKIQRLYQILNKSVSDCNSTCISLSGGLDSSILASLMKSKEK
                     NAISIICKEFPGTDLTYCQMVAKKFQLPLVMKLISTEEVMTAIDKTIKILKVFNDIEI
                     RNSVVMYLSLQTAKNEGHKSIITGDGADELFAGYNFFQKMTEDELKKNLTRIWNTMHF
                     PVQKIGKSLKIIVESPFLNEHVLNFAKSIPIQYNIKIEKKQKFGKWLLRKTFENSIPQ
                     SITWRKKSAMQDGAGISQLVSIFDNIISDESFSDQIKLIKKTDGVSIRSKESLHYYLI
                     YRKYYDIPKNLHTSDSVCKYCHYEVLNDTHFCKMCGAFPI"
     gene            complement(8111..8260)
                     /locus_tag="QGF01_05300"
     CDS             complement(8111..8260)
                     /locus_tag="QGF01_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HJN20335.1"
                     /translation="MPVDPVCGIELDESLALLHEHDGKKYHFCCNGCRRIFVKKPKKY
                     KNNIK"
     gene            complement(8687..9511)
                     /gene="purC"
                     /locus_tag="QGF01_05305"
     CDS             complement(8687..9511)
                     /gene="purC"
                     /locus_tag="QGF01_05305"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJN20336.1"
                     /translation="MKFLTSGKVKDVYDLEDGNLQFKFSDRVSAYDVKFNDEIPKKGE
                     VLCKFAEFWFNELDIPNHFVRRISDTEIIVKKMEMLPIECIVRGYFYGSLVERYNNNS
                     IQLDNVDDISLAEKLPTPIFDPTTKAEHDVPITKIDAIKFGLVSEHEFDWLAKTSIQI
                     YNIMLAKAKSAGFILADLKLEFGKHENELFLSDSIGPDEYRLWLKDDYTVGKIQEAYD
                     KQLLRDWLTDNGFKKKFDDARASGKEPTPPSIPKELVKKMTDRYEKSYELIVNQKL"
     gene            complement(9549..9968)
                     /locus_tag="QGF01_05310"
     CDS             complement(9549..9968)
                     /locus_tag="QGF01_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20337.1"
                     /translation="MAFPYKPKPNHSIQKKFFLGGGICGLIILAIFVIYTAFPSPTAF
                     DDHSIMVDPTVEKQSLFTIARVTIQNTGTSQLTNLTINYGDNDIDSIGTLDPGKTIIL
                     SPPQGNSLQYVIVTADGDVHIFKAYREPVAMPGMMGS"
BASE COUNT         3370 a         1666 c         1376 g         3636 t
ORIGIN      
        1 gaaacttcct aaagtaatca aatgttctcc acccaaaatt attatttgct tgccttgttt
       61 tgctaattca gaagtaatat tttcaaccat tcgtaacatt tctgtggcaa caactgtatg
      121 ctttgtatta ccaagatcat tgatatttac agtttctaga tcaacaccaa attccggttg
      181 aaagatttca atattattaa atgcatcacg tattgcatca ggtccaaaac gacacccagg
      241 tttgtaagaa tgcgttgaat caaatggtat accaaaaata gtaacttttg agtcatcatt
      301 atcattggga ttaataatca atggtgaatt tgtcatgtga agatccaaaa aactcatttt
      361 tttataccat tatttgtggt ctttgtgata atagaagtgt tgaatttaat cttgaagttg
      421 gtttatcact tgtagcatct gtgatttcct tgataaaatc ttcaatagaa acttcacgca
      481 catcgccggt tgtcctatca cgaatactca attttggaga attcacttcc ttttctccaa
      541 taactaagac gtaactaatc cattcctttt ctgcatcacg aattcttttc ccaattgtgt
      601 catttctgtc atcaatatct actcgtatat tttgctcaat aaatttatca gcaagatgag
      661 ttgtaaattc tagatattca tctttaagag gaattaatct cacttgtatg ggtgccaacc
      721 aaagtggaaa ttgtggtctc ttaccatctt tgatctgttt agcaaatttt tctaataatg
      781 cataaatcac gcgttccact gctccagacg gtgaattatg taaaattata ggatgtttct
      841 gctcgttatt ttcattggtg tatgttatac catagcgttt accattttca acatcaattt
      901 gatcagttga taatgcactt gctttaccta aattatctat aaaattaaat tcccatttca
      961 gaacaaaata aaaaaatctc tccttccaca tttcaactaa aactggcttt cctatttttg
     1021 aaaccatttg tttgattaat tctttatttt gattataaaa ttcatcagtg aacctgatag
     1081 ccatttcata ttcattttct tcaattccca atccagataa tacctcacgt gaaagatcaa
     1141 atctttttct aaattcttct attgcttgtg gcatatcttt acaaaatgca tgacaatcag
     1201 gcatggtaaa tgctctcaat cgtcttagtc caacaagttc tccagattgt tcacgtctaa
     1261 aactataacg agtcaattca tagagtttgt atggtaaatt tttgtatgat aattgaaaat
     1321 ctttagtcat taaaaactgc ccgaagcatg ctgcaaacct taagaaaaga tgtttaccct
     1381 ccgaatctat actatactgt cttgcaggaa atctattgaa ataactttcc atgcttggat
     1441 ggtatgaatc atacattatc ggagtttcaa cttcgattcc accataatta cgaattttat
     1501 cggtaacata ttgttctatt tgagatttga ttagacgtcc attaggataa aatctcatat
     1561 ttcctgaatc ggacgcaggt tcataatcag caattgcaag ttttttcatc aagtttacat
     1621 gtggaggcgc ttcatcactg cttcttgttt ttgctgattc atattttgca agtatctcaa
     1681 gctgtttatg attagaaaaa ttaaattttt caatctcctc catttttcct tcaggtgtca
     1741 taatgtacca atatgatttg atcttagatt cagattctaa tgctgtagat gttttttcct
     1801 tgattgaatc tttagaaaaa actttggaac tttctgcaag cggatgaccc ttaacttgta
     1861 tactaaaagc ttttgtccat ccaaatggag catgtgtaac ttcaattcca gtacagattt
     1921 cttgcatttc ctttaaaatt ttaagacctg taccaggaga tgcaaggtca gagcttaaat
     1981 gtgcatatgg ataaagtagt aatttattac atccaacttt tttcattgtt tcttgaatct
     2041 cgatcattgc gttttttgca atatttgagt catcatcttt ctcaactgca ataaaacata
     2101 ctacaacttc ttctatccga gttgattttg gttcaatttc ttctgcagat ttaatttctt
     2161 tctttgtggg tgtgtattca atactgtcac aatgtaattg taaaatacgc ataatttcac
     2221 acaaaatcag ttattgaatt aaatgttagc atgtgggtca tttttcactt tttaataaat
     2281 ttttaaagta tttagttagg ttcgttataa agtccaaact caaatcatct cttttatcat
     2341 caatcatatc aataagatcg tcaatcattt tatctgtttt ttcaatttct ttatcagaaa
     2401 tacctttttt ccattctgtc atttttctaa gatcactttg attttttcct tatcgttcat
     2461 aaaaatagaa aggtcaggaa gtaattaact catttttaat tagcgatgaa agcccaatag
     2521 accgcaacta taactacaac agattcaaca gcgatcaaaa ttttgaaata tgctttcatg
     2581 atattctact tctttctttt cttttggctt tcaatctttt ttgtttttct ttgtaagata
     2641 ttgctgtcat taagattcta agatattttt gatttcagct ctcatttttt tacctgtctt
     2701 agaatattct ttcaattccc aattagcaat taatttcaaa gttttttcga ttttttcctt
     2761 atcgttcata aaaatagaaa ggtcagcaag tacaaaagtc agctgagttc tgaccctgat
     2821 gactttagtt agtttcttct tttcttagct tgtctatcaa taacgaataa tattattccc
     2881 aaagtgacac cagttaatcc tatcatagcc caaagatacc acatacaaca ttgacaaaag
     2941 acatctaata aaaatgaaaa tgattaatca gaaaatgcca tcttcctgat ttgggtgtgt
     3001 acgcctgaaa actcgttttt aattatttac gattagtacc tactctactt cttttcttct
     3061 tactgattct tgatcttctt tcttgttctt tttgtacttt tgttgccatt aagattctaa
     3121 gatttttttg atttcattta atgccctttc agaatctctt ggtgtagaaa gtgtttcatc
     3181 caacactttc ttaatcgcat caattttgcc ctcccagtaa ttgtcgtgtc cttcaatcag
     3241 accacatctc ttttttggtt gattaatcac ccattcctta atattttcag tcattcccaa
     3301 aacaaaatca cttttttcct taaagtccat aaaaatagaa aggtcagcaa gtacttatcg
     3361 atttttgtaa agtttcctca gtctttcaga attttcagac ctatccttag ctgtgacaca
     3421 gaatcttttt ccaatacaat ctttgacatg ttcttttatg ttcttatgac aaaaagggca
     3481 aattatatca ggagtgaaat gacttccctc atattttttg tcttgctcag gttctgtcat
     3541 ttttttgatt cactcttcta agatttcttt gatttctttc aaatctcgca ttgagtaggc
     3601 tatttcattg tacttagatt tcattttaat gaatgcgtca atcttttcca aagctttttc
     3661 gattttttcc ttatcgttca tttttttaat tcactaaact tgagtaattg catgttatgt
     3721 attcaataat ttgtgacaat ctaactaaac cctgctccaa attcatcgcc ttttgtcaat
     3781 ggatcaatat caccgccatc ttttaatttt aaatatagaa atgcctcatg gactaattcc
     3841 ttagccatac tactttctaa actataattt tgtactgcaa aatcatagta tttttttagt
     3901 tttgactgat cttgttcatc aattgacaca tgatcagatg taatcatttg agcaattttt
     3961 tctattctaa gattatgttc tgtatcagac aatacaaaaa ttaacaatta ttgtttaaaa
     4021 aattgtttgg tcattcattt tcatcatgat ctagaatatg attaacacag taatattcat
     4081 cattcatatt acaataataa agtgatttta catcacatag tgagcatgtt ggcttttcat
     4141 catactctga taattttctg tgataaaatt tagatgacat atcaacgatt gtccagaaat
     4201 actaatgagc ggagtattac actagaaata cccaaattat tgatcaaaaa tttactggta
     4261 ttctcaaatt ctttggtatc aaaatagcct tcatcataaa tcatttcgac agaatcttca
     4321 gtggtttcaa ccaatgatgt gattactgaa tatggcccta attctttatc aattttttca
     4381 atatataacc gcaaaaaaat atttcttatt tcataaccat tttctataga attaccatta
     4441 gtcagtattg aaataatctt atctggttca cgttctaatc tttttggatt tggtatatcg
     4501 atatatttca acataatgtc tatgtttaac ataaatttag atttttaata cacagtttgt
     4561 attaagaaaa tttatcatac ctagatctct ctaatgatct atcctccttt gtttcttttt
     4621 tccattcctt ataatgagtt ttacaaagaa cagttttttt ccctgtggct gaaacacgta
     4681 acccagcatt ctcaacttta gaagtattta atgaacgtat cccatcattt ttacaatcgg
     4741 caacattaca ttttgctccc ttagaaataa ttcccataca tgtttttaga ttaatagata
     4801 ataaattaat cttaaatcca aaatgtttga ctatatacga aaggatttct aagaatgacc
     4861 tagatctaaa agccctgtct ttttctacgc actatcgcaa aaaccacaac tgcaataatc
     4921 atgacagttc ccgcaagaaa ataagttaaa agtgaggatt ctgccatact agattatact
     4981 ttcagctttc ttaaaaagaa tattttctta atcaaaaatc ttcgtttata aataggagct
     5041 atattttcac agcaatgggc ggttcaaaaa atatatcacc tgcacaaaaa gaaaaaaagc
     5101 aacaacgtag ttctgataca aaaaaaggta aaaaagatag taaaggtgat aaaaatgaaa
     5161 aagccgagat ttcagtaata ctaaatgaaa cagatgcgga aaaaattatt aaaaatgcca
     5221 aaatcatcac cctatatgat tttgcaagac aaacaggtgt taaaatctct acagcaaatg
     5281 aatttttgca aaagtcatta aaaaatgggg cgataaaaaa gattggtggt tttagcggtc
     5341 atcatatata tcaaccaatt tctgaataga gtgacagtaa atcacccggt ttaatctgtt
     5401 ttagcttttc aatatcagat aatattcttc caataggtgt cattggttta ccaccataca
     5461 catccttgat gtagaaaaaa atacttcctt ctattggaaa aaatgcaata tctcccattt
     5521 taaaatctgt tctttttctt tctattccag aatctatggc agtttccata tagactgaag
     5581 actcactgtt gcgatgtgca ttaccttgta acggtgtaga tcttgtgatc aatccaacag
     5641 tttttggaga aagatgacgt tttaactggc aagagatttt tgatttccca tttatatcta
     5701 aaaataaatt aatgcttgaa acagtatttt gaatcaattt tgatatcaac tactagaata
     5761 tataaccgct attataaact caagaaatgc ctaaatctga taatgatgta gatgaggtaa
     5821 aattagaaga ttcagacgtc gacaaaaaaa ccgatgacgt caaatcaagt tttgaaaatg
     5881 atagtgtaga caatgcaata actgcataca gaaaattatt tgatgtgaaa gatgttgaaa
     5941 ttaccgaaaa acagcaagca gaaattgatc aagtatgtga agttatgaga aagcgtgaaa
     6001 tcatatataa ggaaactgaa catgaaccag aagaaattac ttctaatgat aataaaataa
     6061 ttacaggccc acctatgcta actagatttg aaaaggcacg aattatgggt gctcgtgcac
     6121 tacaattatc attgggtgca ccaccgttta tttctgtacc aaaagacact agtacttcat
     6181 tggaaattgc aatggaggaa ctaaatgaac gtttgatccc tatcgtaatt agaagagtat
     6241 tacctaacgg tgattatcaa aacataccta ttaatcattt caaatagaca aatcatcgat
     6301 aaaatttaaa agaattaaaa aattcataga tttaacttat gacaacagat atggaaagta
     6361 atggctctga tagttttact gatgaaattt ttcttgtaga tagtgatgaa cctataatga
     6421 atattgcttt tgatgcatta gaaatgatca gaaaaaataa aaaaattata gtaaaaggaa
     6481 aaggtgaaat gtgttcaaag gctgtagcag tatctaatat attgacagaa cgtatgctta
     6541 aagaaactgc aaaatttgga aaaattgatg tggatagtga aatgttagat gatgggcaat
     6601 taatttcaac aataaaactt gctatagtta tgacagatta attaataaac actgcctggg
     6661 ccctttagaa gcatattttc acactctttg catattttat catctatgtt tgattcaaga
     6721 ttatgatgaa tgtcacaaat tagaagactg gttttgatat aattacataa acctatgaat
     6781 tttgatagag ttgctggtgt gtaatctttt tcagctgcca atctatcagt tatatcagca
     6841 atcatcttta caatttgttc ttctgctaaa agcttctcaa ttaattcagg attcccacgt
     6901 attccacgaa tataattact cactgccgct tgagtaacac ctaacattcg tgaaatttca
     6961 tcctcacgta tatcatgatt cgcagcaagt tttttagcta atattgcacg taaagctggt
     7021 attagtgtct ttgattcaat ttcagctggt aataacataa tcaaaaatca ttattaaatt
     7081 atttaaatgt actagatcat atcttacagt ttaggaagaa aacctaattg gtaaatgtct
     7141 ttattcattc ttgatttagt agatacgatg aatgagaaaa ttcaaagatt atatcaaatt
     7201 ttaaacaaat ctgtttctga ctgcaactct acatgtattt cattatctgg tgggttagat
     7261 agtagtattt tggcttcact tatgaaatca aaagaaaaga acgccatatc aattatttgt
     7321 aaagaatttc ctggaacaga tctaacatat tgtcaaatgg ttgcaaaaaa atttcaatta
     7381 ccattagtta tgaaattaat cagtacggaa gaagtaatga cagctattga taaaacaatt
     7441 aaaattttaa aggtttttaa tgatatagaa atccgaaatt cagttgtaat gtatctatca
     7501 ttacaaactg caaaaaacga agggcataag tcaataatta caggtgatgg ggctgatgaa
     7561 ctatttgctg gctataattt ctttcaaaaa atgactgagg atgaactaaa gaaaaactta
     7621 acccgtatat ggaatacaat gcactttcct gtacaaaaaa taggaaaatc attaaaaatt
     7681 attgttgaat caccgtttct taatgaacat gtacttaatt ttgctaaatc aatacctata
     7741 caatataaca taaaaataga aaaaaaacaa aaattcggta aatggttact tagaaaaaca
     7801 tttgaaaata gcattcctca atcaatcacg tggagaaaaa aatcagcgat gcaagatggt
     7861 gctggtattt cgcagcttgt gagtatcttt gataatatta tctctgatga atcattcagc
     7921 gatcaaataa aattaataaa aaaaacagat ggtgtaagta taagatcaaa ggaatctctt
     7981 cattactatt tgatatatag aaaatattat gatattccta aaaaccttca cacttctgat
     8041 tctgtttgca aatattgtca ttatgaagtg cttaacgata cacatttttg taaaatgtgc
     8101 ggagcgtttc ctatttaata ttatttttat atttttttgg tttctttaca aatattcttc
     8161 tacatccatt acaacaaaaa tgatattttt taccatcatg ttcatgcaat aacgcaagac
     8221 tttcatccaa ttctattcca catacaggat cgactggcat gcttattctg tcaatataat
     8281 gtataagtat ctactcacgt gcttatatca gctatcaagc agtaaaatca aattcttttc
     8341 tataactttg ccatcttttt catttttgaa aatattaatt atgatggatt aaaattgttt
     8401 taaaattaaa aatcgataac agttgcatta tcttcacaaa tcttctaagc gatttgtaag
     8461 ttagcgttga aacataacca gatttctgac aactgtcatc atattctata cggtaaaata
     8521 ctataaaaca atctatgaaa attatcacag gtgagaaata ctagatggca tttttaccga
     8581 tattacttgt gcaaatatca atagcatttt caacattcaa gataaatggt atgtattcac
     8641 aagtatcctt gattaatttc tgaacttaac atttaagaaa taaaaattaa agtttctgat
     8701 taactattaa ttcataagat ttttcatatc tatcagtcat ttttttaaca agttctttag
     8761 gtatacttgg aggtgtaggt tctttaccag atgctcttgc gtcatcaaat ttcttcttaa
     8821 aaccgttatc tgtaagccaa tcacgtaaca attgtttatc atatgcttct tgaattttac
     8881 caacagtata atcatctttt aaccacagac ggtattcatc aggtcctata gaatcactca
     8941 aaaataattc attctcatgc ttaccaaatt ctagttttaa atcagccaat atgaaacctg
     9001 cagatttagc ctttgccaac ataatattat aaatttgaat tgatgttttt gctaaccaat
     9061 caaattcatg ttcagataca agaccaaatt ttattgcatc aatttttgtt attggtacat
     9121 catgctctgc tttggtagtc ggatcaaata ttggtgtagg aagtttttca gctaatgata
     9181 tatcatctac attgtctagt tgtatagaat tattgttata acgttctacc aaacttccgt
     9241 aaaaataacc acgtactata cactcaattg gcaacatttc catttttttt acaattattt
     9301 ccgtatcgga aattcttcta acaaaatgat ttggtatatc caattcatta aaccaaaatt
     9361 cagcaaattt gcacaaaacc tctccttttt ttggtatctc atcattaaat tttacatcat
     9421 atgctgaaac cctatcactg aatttgaatt gtagattacc atcttctaaa tcataaacat
     9481 cttttacttt gcctgaagtt aaaaatttca atgtgaatca caatatcatt ttatttaggg
     9541 tatgtaaatt atgaacccat cataccaggc atagcaacag gttctctata tgctttgaaa
     9601 atatgaacgt caccatctgc agtaactata acatattgta atgaatttcc ttgagggggt
     9661 gacagaatta tagttttacc aggatccaat gtaccaatag aatctatgtc attatcacca
     9721 taatttattg taagatttgt caattgacta gtgccagtat tttgaattgt gactctagca
     9781 attgtgaaaa gactttgttt ttccactgtt ggatcaacca taattgaatg atcgtcaaat
     9841 gcggttggag atgggaaagc tgtataaatc acaaatatag ccaatatgat taatccacaa
     9901 ataccaccgc ccaaaaaaaa ttttttctgt attgaatgat taggttttgg tttgtatgga
     9961 aatgccatat tatgaatatg atttcttctt aataaaatta aatggtcgat taatcataat
    10021 tattgatgat gttcatcatc tttcttgc
//
LOCUS       DASCPJ010000109         4923 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001743323, whole genome shotgun sequence.
ACCESSION   DASCPJ010000109 DASCPJ010000000
VERSION     DASCPJ010000109.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001743323"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            66..260
                     /locus_tag="QGF01_05315"
     CDS             66..260
                     /locus_tag="QGF01_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HJN20338.1"
                     /translation="METGTVKWFNQTKGFGFIERENSEKDLFVHKTEVEGSIRDGDKV
                     EFEVGETEKGPNAVKVKAVE"
     gene            complement(418..1755)
                     /locus_tag="QGF01_05320"
     CDS             complement(418..1755)
                     /locus_tag="QGF01_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJN20339.1"
                     /translation="MIPSKVLIIGAGVSGLSTAALLANENISSIIYEKSSSVGGRTST
                     TIHNGHILDNGFHIMPFYKKSKIYEILEKINMIEKLNLVNVYDIAFYDGDKFHKYPKG
                     IIDILQLTLIPFNSRLSLLRILLPMAFTSIEKTEQLDKTSLTEVTKKLDNASKSFFDA
                     VCMLAFADTAENISLGEFTRTIIRANPFKGGTSEFAYPDMGGYDRIAKLFAEYSIKKG
                     SKINLGKQVKKVEVENDKVKGITLTDGQFVSSECVIVTFPSYLAVNQLFDDDVFDKHF
                     LESVNRLNQTTSVIEVHFALSKKIDSRQIVFPVGQNYISKGIFFISNITSSVSPAGEH
                     LMMVGTPVHAEEAKSSKRIREITEKMKNELNIIYPEFQRVLLWERPKAWSLVESVVKK
                     PGLVWKNKMPHEVPHIKGLFFVGDSTVSYGIGTDSAAHSSILCHPKIMSYLKQ"
     gene            complement(1952..2794)
                     /locus_tag="QGF01_05325"
     CDS             complement(1952..2794)
                     /locus_tag="QGF01_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014375.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HJN20340.1"
                     /translation="MVFLRHKIVKGNSYTYLVENKWNSEKKTPMQKTIKYLGKTSSII
                     LSDIPYQYRNSASVITFLGSTKNTRLVDRERHIRKLKKDFFEFLIHGEIDRVQAIYRN
                     FTRDSTLSDFYQNILRPVLYMIGDMWDLKKLDVGEEHIASNTVVRLLELIKKETRSKP
                     SIGKNVLICTPYSENHVIPCLMLETFLSIRGYKVINLAPSVPVKSILNQIETKKPDLV
                     LMSITLSDHLESGKRLITKIKKLKIPILIGGQATIDMKKIPGVAYIESDSLQKIAQLV
                     KQVI"
     gene            complement(2796..3746)
                     /locus_tag="QGF01_05330"
     CDS             complement(2796..3746)
                     /locus_tag="QGF01_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20341.1"
                     /translation="MKLILLTLAALFSLVIMIPSVHAQVTTSSNYEPNDCRVCVPDWI
                     KNNAGWWADGIIDNSSFISGIEWLVSNDIIQVPSTAVSDAAESSIPDWVKNTAGWWAG
                     DIISDDDFVNSIQYLIKVGIMTVPQGEKVVPETTSMPTDADPKLIREADELKSKIDAC
                     QQKDTEADIYLCSSKFEKQLAIIELMMEASKFQAGPVDFYYKPALVEISPYGNSFVEI
                     ILVMRNTDTEIQSLFCTGPEACNITFTDGKKSWKYAANDIPTGRLAMVPDNIYTIKIS
                     YGPPPQKQYDRWHYDSSSEYFLKINESYGNVSIPLELTVK"
     gene            3867..>4923
                     /locus_tag="QGF01_05335"
     CDS             3867..>4923
                     /locus_tag="QGF01_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HJN20342.1"
                     /translation="MLDNLKSSLRAAIKKIVNSSGIDEELIKELSKDIQRSLLQSDVN
                     VKMVFEITKNIENRSLNETPPPGLSRKDHIVKILYDELAKLLGNETEFHFQSGKVNKV
                     LMLGIQGSGKTTVTSKLAKFLTKQGYRVAVIGADTFRPGALTQLKTMCEKSDVEVYGE
                     NGNENPSEIVKNGLKHFQKTNLDIILIDTAGRHKQEKDLLDEMKQINKTADPDLALLV
                     IDGTIGQQCFTQAEAFHKTVPVGGIIITKLDSSAKGGGALAASAATGAQVMYIGTGER
                     IDDLEVFSPTRFVGRLLGMGDIQAVLDLAKRLENEADDVRMKRISSGKMNMEDFYYQL
                     EEVTKVGSFQGLLDTMPG"
BASE COUNT         1612 a          801 c          800 g         1710 t
ORIGIN      
        1 ttgtttgatt gtggagagtt actgagtaaa gggtttatac gtagtctttt ttaagatatt
       61 tcaatatgga aacaggtacc gtaaaatggt ttaaccaaac aaaaggattt ggtttcatcg
      121 aacgagaaaa ctctgaaaaa gacctctttg ttcacaagac agaggttgag ggttcaattc
      181 gcgatggaga caaagttgag tttgaagttg gagaaaccga aaaaggccca aacgcagtga
      241 aagtaaaagc agtagagtaa gattaacaat tttttaataa attttcttaa tttctataaa
      301 ataactaact gtataatatc catttttttt taaaaatccc tagtgataat gctgaccgaa
      361 tacacattac aagaaattcc taatctattt taaatcacaa aataaaataa taaataacta
      421 ttgtttcaag taggacatta tttttggatg acataagatc gaactatgtg cagcggaatc
      481 tgttcctatt ccataactga ctgtagaatc accaacgaag aaaagaccct ttatgtgtgg
      541 cacttcgtgc ggcattttat ttttccatac taatcctggt ttcttaacca cagattcaac
      601 taaactccat gcttttggtc tttcccacaa taacactcgt tgaaattctg gatagataat
      661 attcaactca ttcttcattt tttctgtgat ttctctaatt cttttactac tttttgcttc
      721 ttcagcatgt acaggtgtac caaccatcat aagatgttcg cctgctggag aaacagatga
      781 tgtgatattt gaaataaaga atattccctt tgaaatatag ttttgtccta cgggaaaaac
      841 tatctgcctc gaatcaattt ttttagataa agcaaaatgt acttctataa ctgatgtagt
      901 ttggtttaac ctattcacag attctaaaaa gtgcttatca aatacatcgt catcaaatag
      961 ttggttgaca gctaaatatg atggaaaagt aacaattaca cattcactag acacaaactg
     1021 tccatcagta agagtaattc cttttacttt atcattttcc acttcaactt tctttacttg
     1081 ttttcctaag tttatctttg agcctttttt tatggaatat tcagcaaata attttgcaat
     1141 tctatcatat ccacccatat cagggtaagc aaattcactg gtaccacctt taaacgggtt
     1201 agccctaata atagtacggg taaactcgcc taatgatata ttttctgcgg tatctgcaaa
     1261 tgccaacatg catactgcat cgaaaaaaga ttttgatgca ttatcaagtt ttttcgttac
     1321 ttctgtaaga cttgttttat caagttgctc tgttttttca atactagtga acgccatagg
     1381 caataaaatt ctaaggagtg aaagcctact attgaatggt attaatgtta gttgtaaaat
     1441 atctataatt ccttttggat atttatgaaa cttatctcca tcataaaatg caatgtcata
     1501 gacatttaca agatttaatt tttctatcat gtttattttt tctagaattt cataaatttt
     1561 tgatttttta tagaatggca ttatgtgaaa tccattgtct aatatatgcc cattatggat
     1621 tgttgtactt gttctacctc ctaccgaaga tgacttttca taaattatag atgatatgtt
     1681 ttcattagca agcaaagcag cagttgataa tccactcact cctgcaccta taatcaatac
     1741 ttttgaagga atcaatctat taacatttca tcttttactt cttgatttag tgcgaagttt
     1801 tactctgcaa catggacatc taaaatcttc tatgttcata aatattgcac actcggtaca
     1861 tcgttttagc ccagaagaat atttttcacc attttgtaat gatgtatgtt tgaactgtaa
     1921 acaaattccc ttacaagtaa ctcccaattt attatatcac ctgttttact agttgtgcta
     1981 ttttctgtaa agaatctgat tcaatgtatg caacaccagg aatttttttc atatctattg
     2041 tagcctgacc gcctatcaat attggaattt ttaatttttt gatcttagta attagtcgtt
     2101 taccagattc taggtgatca gataaagtaa ttgacatcaa aaccagatca ggtttttttg
     2161 tttcaatttg attgagtata gattttactg gtacagatgg agccagattg attactttgt
     2221 atcctctaat tgaaagaaaa gtttctaaca ttaaacatgg tataacatgg ttttcagaat
     2281 atggtgtaca gattaataca ttttttccta tactaggctt agatcttgtt tcttttttta
     2341 tgagttctaa taggcgcaca acagtattac ttgcaatatg ctcttcgcca acatcgagtt
     2401 tttttaaatc ccacatatca ccaatcatgt ataatactgg tcgtagaata ttttgataaa
     2461 aatcagataa tgtagaatct cttgtaaaat tcctatagat agcttgaact ctatcaatct
     2521 ctccatgaat cagaaattca aaaaagtctt ttttcagttt acgtatgtgt ctttcacgat
     2581 ctactaatcg ggtgttttta gtagaaccaa gaaaagtaat gactgatgct gaatttctgt
     2641 attgatatgg aatatctgat aatataatac tagatgtttt tcctaaatac ttgatagttt
     2701 tttgcatcgg tgtttttttt tcagaattcc atttattttc tacaagatat gtgtatgaat
     2761 tacccttgac aattttatgt cgtagaaata ccatgttatt ttacagtcag ttctagaggt
     2821 attgagacat ttccatagga ttcgttaatt tttagaaaat attcactcga tgaatcataa
     2881 tgccatcgat catattgttt ttgtggagga ggaccataac tgattttaat ggtgtatata
     2941 ttatcaggga ccattgctaa acgtcctgtt ggtatatcat ttgcggcata cttccacgat
     3001 ttttttccat cagtaaatgt tatgttgcag gcttctgggc cagtacagaa taatgattga
     3061 atttcagtat ctgtatttct cattaccaaa attatctcta caaatgaatt accatatggc
     3121 gatatttcta ctaatgcagg cttgtagtaa aagtcaactg gaccagcttg aaatttactc
     3181 gcttccatca ttaattcaat tattgctaat tgtttttcaa atttagatga acataaatag
     3241 atatcagctt ctgtatcttt ttgttgacat gcatctattt tagattttaa ttcatcagct
     3301 tcacgtatta atttgggatc tgcatcagta ggcatacttg ttgtttcagg aacaactttt
     3361 tcaccttgtg gaacagtcat aattccaacc ttaatgagat actgtatact gttaacaaaa
     3421 tcgtcatcag aaataatatc accagcccac caaccagcag tgttttttac ccaatctggt
     3481 atagatgatt ctgcagcatc agatactgca gtagatggca cttgtataat atcatttgaa
     3541 acaagccatt caatacctga gatgaatgaa ctgttgtcaa ttattccatc tgcccaccac
     3601 cctgcattat tctttatcca atcaggaaca caaactctac agtcatttgg ttcataattg
     3661 cttgaggttg ttacttgagc atgaacactt ggtatcataa tcactagtga aaataatgca
     3721 gccagtgtaa gcaaaatcaa cttcatgcct atttttagtt caccagaata taaatagtgc
     3781 taaaattaca tactagtgct aacaaaaaat caaagatatg taatttagat ggattagttt
     3841 tttagtaatc aatttttcat aaaaacatgt tagacaattt aaaatcaagt ctaagggcag
     3901 cgatcaaaaa aatagtcaat tcttcaggta tagatgaaga attaatcaag gaattgtcaa
     3961 aagatattca gcgttcatta ctacaatctg atgtaaatgt aaagatggta tttgaaatca
     4021 ctaaaaatat cgaaaatcgc tctttaaatg aaactccacc tcctggacta tccagaaagg
     4081 atcatattgt aaaaatactg tatgatgaac ttgcaaaatt attagggaat gaaaccgaat
     4141 ttcattttca atcaggtaaa gtcaataaag ttctaatgtt gggtattcaa ggtagtggaa
     4201 aaaccacagt tacgtctaaa ctggcaaaat ttttaacaaa acaaggctat cgtgttgccg
     4261 ttataggtgc tgatacgttt agacctggag cacttactca actaaaaact atgtgtgaaa
     4321 aatctgatgt ggaagtttat ggagaaaatg gtaatgaaaa cccatcagag attgttaaga
     4381 acggtttaaa acattttcaa aaaacaaatc ttgatataat tttgatcgat acagctggaa
     4441 gacataaaca agaaaaagat ttgttggatg aaatgaaaca aatcaataaa actgctgacc
     4501 cagatcttgc cttattagta attgatggta ccattggaca acaatgtttt acacaagcag
     4561 aggcttttca taagactgtt ccagtaggtg gtattataat caccaagctt gacagttcag
     4621 caaagggtgg aggtgcatta gcagcatctg cagctactgg cgcacaggtc atgtatattg
     4681 gaactggtga acgtattgat gacttggaag tgttttcacc aactcgtttt gttggaaggt
     4741 tacttggtat gggagatatt caagctgtgt tggatctagc aaagcgatta gaaaatgaag
     4801 ctgatgatgt aagaatgaaa cgtatttcaa gtggaaaaat gaatatggaa gacttttact
     4861 atcaactgga agaagttaca aaagttggtt cctttcaagg gttattggat actatgccgg
     4921 gtt
//
LOCUS       DASCPJ010000110         2385 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001767317, whole genome shotgun sequence.
ACCESSION   DASCPJ010000110 DASCPJ010000000
VERSION     DASCPJ010000110.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001767317"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="QGF01_05340"
     CDS             <1..719
                     /locus_tag="QGF01_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HJN20343.1"
                     /translation="LVRRRGRGGMQFRASTTGKLAPAKYPSFQLTENHVGIIIDLVHE
                     SGRDVPLAKVRFNDGSISFLPAVIGTKVGSKIQFGLKATITDGNVTSIQNIPDGTTVC
                     NVEKQFGDGGAFIKSAGTDATVFSHEDDGVKLKLASGKFTKVHPKNRAMIGTLAGGGV
                     GDRPFMRAGVKWRRFRSKGQKYPIVRGVAQASYVHPHGGGRHQHVGQSSTVSRNAPPG
                     AKVGSIAARKTGRARIKERK"
     gene            763..1041
                     /locus_tag="QGF01_05345"
     CDS             763..1041
                     /locus_tag="QGF01_05345"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HJN20344.1"
                     /translation="MDKRRVHIVIGGKVQGVFFRQALKVMAKKNNVFGWVKNLEDKRV
                     EALLEGNTENINSIVKWARIGPAHSRVDNIEINDEKFKNEFSIFEVLY"
     gene            complement(1066..1377)
                     /gene="ffs"
                     /locus_tag="QGF01_05350"
     ncRNA           complement(1066..1377)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="QGF01_05350"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(1417..2322)
                     /locus_tag="QGF01_05355"
     CDS             complement(1417..2322)
                     /locus_tag="QGF01_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HJN20345.1"
                     /translation="MICNKCQNQAVYSRKYSGENLCSECFSDSILRKAARTISKYKMI
                     KNNDLVCVCVSGGKDSLVLLDILNKMSKTHNFKIFAVSIDEGIPGYRDEALKIVENFC
                     AELKIKYKIFSYKKLFDLTLEESLDLRKDNNSSCSICGIFRRRAFDHAAKTINADVIA
                     TGHNLDDVLQTFLINTTSGDTSKIGWMHPDASSNKLRKIKPLSEIYESEIVFYAFTND
                     LPFQTEPCPHMDEGIRTEIREFLNSLESNHSGIKNNMYNTILKLSQFTNQSIQKEKRT
                     CLICGNECTGKICSVCNMITDLKGK"
BASE COUNT          758 a          415 c          437 g          775 t
ORIGIN      
        1 ctcttgttag aagacgtggc cgtggaggca tgcaatttag agcatccact actggaaagc
       61 tcgcacctgc aaaatatcct agttttcaac ttactgagaa tcatgtaggc ataataattg
      121 atcttgttca tgaaagtggc cgtgatgtcc cattggcaaa agttagattt aatgacggtt
      181 ctatttcatt tcttccagca gtaattggca caaaagttgg ttcaaaaatc caatttgggc
      241 tgaaagccac aataacagat ggtaatgtaa ctagtattca aaacattcct gatggaacta
      301 cagtttgtaa tgtggaaaaa cagttcggtg atggcggcgc ttttatcaaa tcagctggta
      361 cggatgcaac agttttctca cacgaagatg atggagtcaa attaaaactc gcctctggta
      421 aatttactaa agtccatcca aaaaacagag caatgattgg tacacttgct ggtggtggtg
      481 ttggagacag accatttatg cgtgcaggtg taaaatggag aagatttaga tcaaagggac
      541 agaaatatcc aattgttaga ggagttgctc aggcatcata tgttcaccct catggaggtg
      601 gaagacatca acatgttggt caaagttcaa ctgtttcacg taatgcacca cctggtgcaa
      661 aggtaggaag tattgctgca agaaaaactg gaagagcaag aattaaagaa aggaaataat
      721 tttcttctta aaactgatta agagctttct ttgtaattgt acatggacaa acgtagagta
      781 catattgtaa ttggtggtaa agtccaaggg gtttttttta gacaagcatt aaaagtgatg
      841 gctaaaaaaa ataacgtgtt tggttgggtt aagaatttag aagataaacg tgtagaagca
      901 ttacttgaag gtaacactga aaacataaat tcaattgtaa agtgggctcg tatcggtcct
      961 gcgcattcac gtgtagacaa tattgaaatt aatgacgaaa aattcaaaaa tgaattttca
     1021 atttttgaag ttttgtatta agatttttgt tcagattaat aatggtagta gctctgctga
     1081 ttccagacta ggtcaataac cccatttcaa ggcgtacaag atccgccacg ttgacgagga
     1141 agcgtgaatg ttccatctta ggattgctcc ctcccggacc tcatcccttt cgatggttcg
     1201 gtcttagaag ttatcccttc ggataattct tgtcctacaa ccacccgcct cggcgtgatt
     1261 tcatttccgc acaccaagcg ggaactacca gcctagagat ttactcagca gttactgatt
     1321 tctgcatata gccggtttca gaacagggca cggctggcac cctaatctta cctactaccg
     1381 ttcatattac aaactggtgt tatatttgaa ttaggatcat ttacctttca aatcagttat
     1441 catattacac acagagcaga tcttaccagt acattcattt ccacaaatca ggcaagttcg
     1501 tttttctttc tgtatggatt gattagtaaa ttgtgaaagt tttaaaattg tattatacat
     1561 attgttttta attccactat gatttgactc tagtgaattt agaaattcac ggatttcagt
     1621 tctaatacct tcatccatat ggggacatgg ttcagtttgg aaaggaagat cattcgtaaa
     1681 tgcataaaag acaatttcag attcataaat ctcagataat ggctttattt ttctcaattt
     1741 attagaagat gcgtctggat gcatccagcc aattttacta gtatctcctg atgtggtatt
     1801 aattagaaat gtttgtagta catcatctaa attatgacct gttgcaataa catctgcatt
     1861 aatggttttt gcagcatgat caaaagctcg acgtctaaat attccacaaa tagagcatga
     1921 agagttgtta tctttcctca aatctaatga ttcctccaag gttaaatcaa atagtttttt
     1981 gtatgaaaaa atcttgtatt ttatttttaa ttcagcacaa aagttttcta caatctttaa
     2041 tgcttcatct ctataacctg gaattccttc atcaatactc actgcaaata ttttaaaatt
     2101 gtgcgttttt gacattttat ttagaatatc taataatacc aaagaatctt ttccccctga
     2161 tacacaaaca cagacaagat cattattttt gatcatctta tattttgaaa ttgttcttgc
     2221 agctttccgt aatatagaat cagaaaagca ctcagaacaa agattttcac cagaatattt
     2281 tctagagtat accgcctgat tttggcattt gttacaaatc atgtatttgc attatttttc
     2341 aataatttag gtgttaatag atcagaatct atttcatctg aaagt
//
LOCUS       DASCPJ010000111         6837 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001770161, whole genome shotgun sequence.
ACCESSION   DASCPJ010000111 DASCPJ010000000
VERSION     DASCPJ010000111.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001770161"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="QGF01_05360"
     CDS             <1..170
                     /locus_tag="QGF01_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20346.1"
                     /translation="ENYQESKMILSESGIKSAEDVKFLRDCGADAFLIGTSIMKSSDI
                     QNSVSKLVNAI"
     gene            167..1357
                     /gene="trpB"
                     /locus_tag="QGF01_05365"
     CDS             167..1357
                     /gene="trpB"
                     /locus_tag="QGF01_05365"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HJN20347.1"
                     /translation="MKIGMPKDGKFGDFGGKYIPETLVPAIEELEENYQKIKNDKSFQ
                     KEFEYYLKNYAGRPTPLYFAENLTKFVGGAKIYLKREDLLHGGAHKINNTLGQALLAK
                     RMKKKRIIAETGAGQHGVATAMACSCLGLPSEVYMGYRDTVRQQQNVFRMDLLGCKVY
                     PVKSGSQTLKDAINEAIRDWITNVDTTYYLLGSAVGPHPYPVMVRDFQSVIGKEIKKQ
                     MKNIPDTVIACVGGGSNAIGTFYPMIGNNVEMIGVEAAGQGLKTGRHSATLNAGKKGV
                     LHGMMTYLLQDDDGQVKETHSISAGLDYPGVGPEHAFLKDAGNVKYKSATDREVINAF
                     LTLTKTEGIIPALESAHAVSHAMKIAKTRSKSDSIVVTLSGRGDKDIDIVKQYLRKNA
                     KNTR"
     gene            1338..2141
                     /gene="trpA"
                     /locus_tag="QGF01_05370"
     CDS             1338..2141
                     /gene="trpA"
                     /locus_tag="QGF01_05370"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HJN20348.1"
                     /translation="MPKIQDKFKELKSKNEKALISYIMTGFPNEHTTLSIVRGLVNGG
                     TDIIELGFPFSDPIADGPVIQNASTVSLNKGAKIEKFFRIVKKIRKETDIPLVLMTYT
                     NILYAHGYTKFMSRVKNAGIDGLILPDMSIEESKNYLKSAKKNNLDTIFLVSPNTNND
                     RLKKIAKVSTGFLYMVAVFGTTGVQTKIHKYTIDSIKNAKKLIGDKLPIGIGFGISTP
                     NDVKKYASVGVDAVIVGSANLKIIENTPTAKLQKRITEYTKRLKRSTRV"
     gene            2178..3773
                     /gene="pyrG"
                     /locus_tag="QGF01_05375"
     CDS             2178..3773
                     /gene="pyrG"
                     /locus_tag="QGF01_05375"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HJN20349.1"
                     /translation="MQTRFIFVTGGVMSGLGKGVVSSSISKLLQLSNQKVSCIKIDPY
                     LNFDAGTMNPIAHGEVFVTEDGGECDMDIGNYERFLNQDIPKSHNITTAQIYSNVINA
                     ERKGEYLGACVQIIPHVTDEIKKRIMTIAEDENLDVLIVECGGTVGDIESLPFLEALR
                     QIKLEGGSENVIFVHVTLAPSLDVVGEQKTKPTQHSVQELRRIGIQPDILCVRCTQPL
                     LEKTRKKISLFTNVSSDDVFSCHDVESIFMIPHVLYEQKLVEKIFSKFAKVGYMNTSE
                     NWSEWNSIVNSLHQSKDPIKIAMVGKYVTLADSYVSVNQALKHAGASIGRQVNIDWID
                     SETFNGNLEKLSNFDGVIVPGGFGTRGSEGIINAANFALKNNLPYLGICFGFQLAAVA
                     YARSKCNLENANSAEISPDTSYPIIDLLPDQNNENDMGGSLRLGANKIDISKNSIAHK
                     IYDSETIFRRHRHRYEFNTKFRTEFEKNGMIFSAESDGGRRMEILEVPSHKFFFGVQF
                     HPEFNSRPGFPEAAFKTFVNASA"
     gene            complement(3776..4777)
                     /locus_tag="QGF01_05380"
     CDS             complement(3776..4777)
                     /locus_tag="QGF01_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJN20350.1"
                     /translation="MRIGISGTQSTEVVAKSVKETLSDSGFESFYFKNNSKALTADLV
                     IVLGGDRGVRNYFHHALDVDTPVLGISESESNAFLAQIELKELSRYLKRIKEQNYIIE
                     NVPRIGVKIDGKDVYPVLNDVAVFTSKSATLMEHVLRINDEEVWHDSSDGVIISTPIG
                     SSAYSMSAGGPVIFQDSNVFGIISVNSLDITRRPLIVSDNSLIEIDEISSRLHCDVVL
                     DGIDRFKINNRIEATKFTPPARIIRMKTDSTTISALAKKVKLAEELFAMPPSSKLLLK
                     ILEYEGSMTQKELAVKTLLPDRTVRLALRHLMEKGYIKRKVSIRDARQKIYEISKLD"
     gene            complement(4809..5681)
                     /locus_tag="QGF01_05385"
     CDS             complement(4809..5681)
                     /locus_tag="QGF01_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HJN20351.1"
                     /translation="MKLDIFSHCVIDHIHIDNSIFTVPGGPACYCSLTARALKFDVKL
                     HTKYGSDFPLADNLTQNKISINNGLSEKLTTQFTLKIHDSERILFLENMCDSIDYDEL
                     ISDNVLISPVFDEISSDLLEKLKSISGFIFLDPQGFLRRKGINNKIYLESTDLNLSKI
                     SAIKTSPDELNALTGISTIDGMNVLQKKGIEYVILTDKQNISLLFNGKIYSLKLPNIE
                     LYDTTGIGDIFSTTFCCTIIKEKDFLWALSFAAGSAQAALENKEIGLDKIPSRSAIET
                     NATYFYNTVKFREI"
     gene            5720..6007
                     /locus_tag="QGF01_05390"
     CDS             5720..6007
                     /locus_tag="QGF01_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S26e"
                     /protein_id="HJN20352.1"
                     /translation="MPLKRASRGRKKGGKGSSGTVQCTNCGQTVPKDKAKKVTSRLNL
                     VEHQLAKELRAQGTYIASPRILKWYCISCAIHFKILKIRSSSKRREHTKLR"
     gene            complement(6010..6582)
                     /locus_tag="QGF01_05395"
     CDS             complement(6010..6582)
                     /locus_tag="QGF01_05395"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HJN20353.1"
                     /translation="MLNNLRSSLQPHLEKIGKTFAATGISPNGWSCIGLIFAFASAFA
                     YGWNIEFSLIIGGIILLIAGFFDIVDGQVARISQKVTRTGGFLDSIFDKIAEVAIFLG
                     ILVGGFAEPYLVFLAITLSLLVSYTRSRAESLGVKLQGIGIGERAERLLVIAIIGIIG
                     FMEYAVIIVIIIAAITFIQRIIVTAKELKK"
BASE COUNT         2475 a         1054 c         1162 g         2146 t
ORIGIN      
        1 tagaaaatta ccaggagtct aaaatgatat tgtctgaaag tggaatcaag tcagcagaag
       61 atgtaaaatt tttacgtgat tgtggtgcgg atgcatttct tataggtaca agtataatga
      121 aaagctcaga tatacaaaat agtgtatcaa aattggtgaa tgcaatatga aaataggaat
      181 gcctaaagat ggaaaatttg gagattttgg tggaaaatac attccagaaa cacttgttcc
      241 agctattgaa gagcttgagg aaaattatca gaaaattaaa aatgataaaa gtttccaaaa
      301 agaatttgaa tattatttga aaaattatgc aggaagacca acaccgcttt attttgcaga
      361 aaatctaaca aaatttgttg gaggtgcaaa aatctacctc aagcgagagg atctattgca
      421 tggtggggct cataaaatta acaatacact tggtcaagca ttacttgcaa aaaggatgaa
      481 aaaaaagcga ataattgctg aaacaggtgc cggacaacat ggtgttgcaa ccgcaatggc
      541 atgttcatgt ttgggattac cctcagaggt ttacatggga tatagagata cagttagaca
      601 gcaacaaaat gtttttagaa tggatttact tggttgcaaa gtctatccag taaaatcagg
      661 ttcacaaaca ctcaaagacg caattaatga ggcaattcgt gactggatta caaatgtcga
      721 tacaacatat tatttactag gttctgctgt tggcccacac ccatacccag taatggtacg
      781 tgactttcaa tctgtaatag gaaaagaaat aaaaaaacaa atgaaaaata ttcctgacac
      841 ggttattgca tgcgttggtg gtggatcaaa tgcaattggt acattctatc caatgatagg
      901 taataatgtg gaaatgattg gcgttgaagc agctggacag ggattgaaga ctggtagaca
      961 ttctgctaca ctcaatgctg gaaaaaaagg ggtattacat ggtatgatga catatctact
     1021 acaagatgat gatggtcaag tcaaagaaac acacagtatt tcagcaggat tagattatcc
     1081 gggtgttgga ccagaacatg catttttgaa agatgcaggg aatgtaaaat ataaaagtgc
     1141 aacagaccgt gaagtgatta acgccttttt aacactaaca aaaactgagg gaataatacc
     1201 agcattagaa tctgcacatg cagtttcaca tgcaatgaaa attgctaaaa ctagatcaaa
     1261 atctgattca atagtagtta cattatctgg acgtggtgac aaagacattg acatcgtaaa
     1321 acaatatttg aggaaaaatg ccaaaaatac aagataagtt caaggaatta aaatccaaga
     1381 atgaaaaagc gttaatttcg tacattatga caggttttcc caatgaacat acaacactat
     1441 caattgttag aggtcttgtt aatggtggta ctgatattat tgaactggga tttccatttt
     1501 cagatccaat agcggatggg ccagttatac agaatgcaag tacagtttct ttaaacaagg
     1561 gagcaaaaat tgaaaaattt tttagaattg tgaaaaaaat taggaaggag acagacatcc
     1621 cgcttgtttt aatgacatat acgaacatac tctatgcaca tggttataca aaatttatgt
     1681 caagagtaaa gaatgctggt attgatggtc taattctgcc agatatgtct attgaagaat
     1741 caaaaaacta tctcaaatct gcgaaaaaaa ataatcttga taccatcttc ttggtatcac
     1801 caaatacgaa taatgataga ctgaaaaaaa ttgcaaaagt aagtacaggt tttttgtata
     1861 tggtagcagt ttttggtaca actggtgtac agacaaaaat tcacaaatat actatagatt
     1921 caatcaaaaa tgctaaaaaa ctgattggtg ataaactacc aattggaatt ggttttggca
     1981 tatcaactcc taatgatgtg aaaaagtatg cttcagtagg tgtggatgca gtgattgttg
     2041 gaagtgcaaa tctaaagatt attgagaaca cacctacagc aaaattacaa aagaggatta
     2101 ccgagtatac aaaaagactg aaaagaagta caagggttta ggaaaagcta cataaatggg
     2161 aaaccatttc catatatatg cagactaggt tcatctttgt aacaggcgga gttatgtccg
     2221 gtcttggtaa aggcgtagtt tcatcttcaa tctctaaact tttacaactt tcaaatcaaa
     2281 aagtttcatg catcaaaatt gatccatatc taaattttga tgctggtaca atgaatccta
     2341 ttgcacatgg tgaagtgttt gtcacagaag atggtggtga atgtgatatg gatattggaa
     2401 attatgaaag atttcttaat caagatatac caaaatctca taacattact actgcacaaa
     2461 tctattctaa cgtaataaat gcagaaagaa aaggtgaata tctaggtgca tgtgtgcaaa
     2521 ttatccctca tgtcacggat gaaattaaaa aacgaataat gactattgct gaagatgaaa
     2581 atctagatgt attgattgtt gaatgtggtg gtactgtagg agatatagaa agcctacctt
     2641 tccttgaagc attaagacaa ataaaattag aaggtggttc agaaaatgtt atttttgttc
     2701 atgttacatt agcgccatca ttagatgtag ttggcgagca aaaaacaaaa ccaactcaac
     2761 acagtgtaca agaactaaga agaattggta ttcagcctga tattttatgt gtaagatgta
     2821 ctcaaccact attagaaaaa actcggaaaa agatttcact atttacaaat gtttcatcag
     2881 atgatgtatt ctcatgtcat gacgtagaat ctatctttat gattcctcat gtgttatatg
     2941 aacaaaaatt agttgaaaaa atattcagca agtttgcaaa ggttggatac atgaatacat
     3001 cagaaaactg gagtgaatgg aattctattg tcaattcatt acatcaatct aaagacccga
     3061 taaaaatagc aatggttgga aaatatgtta cactagctga tagctatgta agtgtaaacc
     3121 aagctctaaa acatgctgga gcatcaattg gaagacaagt aaatattgat tggatagact
     3181 ctgaaacttt taatggtaat ctagaaaaac tttcaaattt tgatggtgtt atagtcccag
     3241 gtggctttgg cacacgagga tctgaaggta tcataaatgc agcgaatttt gcattgaaaa
     3301 ataatttacc atatcttgga atttgttttg gttttcaatt agctgcagtt gcatatgcta
     3361 gaagtaaatg caatcttgaa aatgcaaatt cagcggaaat ttctcctgat acatcatacc
     3421 caataattga tctacttcca gatcaaaata atgaaaatga tatgggtggt tcgttacgtt
     3481 taggcgcaaa taaaattgat attagtaaaa attcaattgc acataaaatt tatgactctg
     3541 aaacaatttt tagaagacat aggcatagat atgaattcaa tacaaagttt agaacagagt
     3601 ttgaaaaaaa tggaatgatt ttctcagctg aaagtgatgg tggaaggaga atggaaattt
     3661 tagaagttcc atcacataag ttcttttttg gtgtacaatt tcatcctgaa tttaacagta
     3721 gacctggatt tccggaagca gcattcaaaa catttgtcaa tgcatctgca tagaattaat
     3781 ctaatttaga tatctcgtaa atcttttgtc ttgcatcacg gatagaaact tttcgtttta
     3841 tgtatccctt ttccattaaa tgccttaatg ctaatcttac ggttctatct ggtaacaatg
     3901 tttttactgc aagctccttt tgtgtcatag aaccttcata ctctaaaatt tttaatagaa
     3961 gttttgaact aggtggcatc gcaaataatt cttctgcaag tttcaccttt tttgcaagtg
     4021 ctgatatagt agtagagtct gttttcatac gaattattct tgctggtggt gtaaactttg
     4081 tggcttcaat tctattatta attttaaatc gatcaattcc atctaaaaca acatcacagt
     4141 gtaaacgtga tgaaatttca tctatttcta tcaaactatt atcagaaaca attagtggtc
     4201 ttcttgtaat atctaaagaa tttactgaaa ttataccaaa tacattagaa tcctgaaaaa
     4261 taacaggacc accagcagac attgagtatg ctgaagaccc aataggtgtt gagattatta
     4321 caccgtcact actatcatgc catacttctt catcattaat tcgtaagaca tgttccatca
     4381 atgtagcact ttttgatgta aagaccgcta catcattaag tacaggataa acatctttac
     4441 catcaatttt tactccaata cgtggaacat tttctataat gtaattttgt tctttaatcc
     4501 gtttcaagta cctagataat tcttttaatt caatttgggc taaaaatgca ttagattctg
     4561 attcacttat gcctaaaacc ggtgtatcaa catccaatgc atgatgaaaa taattacgta
     4621 ctccacgatc accacctaaa acaatcacta aatcagctgt taaagccttg gaattatttt
     4681 tgaaataaaa tgattcaaat cctgaatcag aaagtgtttc tttaactgat tttgctacaa
     4741 cctcagtaga ttgagtccca gatataccaa tacgcatcat aactaaacga tgattctata
     4801 atataaaatc aaatctctct aaattttacc gtgttataaa aatatgttgc attagtttct
     4861 attgcactcc ttgatggaat tttatcaaga ccgatctcct tgttttcaag tgcagcttgt
     4921 gctgaaccag cagcaaagct caatgcccac aaaaaatctt tttcttttat tattgtgcaa
     4981 caaaatgttg tagaaaaaat atcacctatt cctgttgtat cataaagttc aatatttggt
     5041 aattttaagg aataaatttt accattaaat aataatgata tgttttgttt atccgttaaa
     5101 attacatatt caattccttt tttttgaaga acattcattc catcaatcgt agaaatacca
     5161 gtaagcgcat tcagttcatc aggacttgtt ttgattgcag atattttaga tagattaaga
     5221 tccgtagatt caagataaat tttattattt attccttttc gtcttaaaaa tccttgtgga
     5281 tctaaaaaaa taaaacctga tattgatttt aatttttcca aaagatcaga tgaaatttca
     5341 tcaaatactg gactgattaa aacattatca gagattaact catcataatc aatcgaatca
     5401 cacatatttt ctaaaaacaa aattctttcg gaatcatgaa ttttcaacgt aaattgcgtg
     5461 gtaagttttt ctgataatcc attattaata gaaattttgt tctgtgttaa attatcagca
     5521 agggggaaat ctgaaccgta ttttgtatgt aattttacat caaattttag agctctggca
     5581 gtcaaactgc aataacatgc aggaccgcca ggtacagtga atattgaatt atctatatgt
     5641 atatgatcaa taacacaatg agaaaaaata tcgagtttca tatactcagg attatatgta
     5701 agaggaaata aaaataacta tgccattaaa acgtgctagt agaggtcgta agaaaggtgg
     5761 taagggttca tctggtactg ttcaatgtac aaattgtggt caaactgtac caaaagataa
     5821 agcaaaaaag gtaacatcac gtttgaatct tgttgagcat caattagcta aagaacttag
     5881 ggctcaggga acttatattg catccccacg tattctaaaa tggtattgta tatcatgtgc
     5941 gatccacttt aaaattctaa aaattcgttc ttccagtaaa agaagagaac atactaagct
     6001 taggtaatat tattttttta attcttttgc tgtaacaata attctttgaa tgaaggtaat
     6061 ggctgcaatt attattacaa tgattacggc atattccata aacccgataa ttccaataat
     6121 agcaatgaca agaagtcttt ccgctcgttc accgattcca ataccctgta atttcacacc
     6181 taatgattct gctcttgaac gtgtataact cacaagtaat gaaagtgtaa ttgcaaggaa
     6241 tactaaatat ggttctgcaa atcctccaac aagtattcca agaaatatag caacctccgc
     6301 tattttatcg aatattgaat caaggaaacc gccagttctt gtaacttttt gagaaattct
     6361 agcaacttgg ccatcaacta tatcaaaaaa accagcaata agcaaaatta tgcctccaat
     6421 aatcaatgaa aattcaatgt tccatccgta agcaaaagca gaagcaaaag caaagattaa
     6481 cccaatacat gaccaaccgt ttggagaaat accagttgcg gcaaatgttt taccaatttt
     6541 ttctaaatgt ggctgaagtg aacttcgtaa attatttaac attgaaatca actaagctga
     6601 aattgagtgt atgtataatt ataaaagtat aattcatttt gattcacatt cttatgaacg
     6661 ttagatatac ttatcttcta tttcatgcct gttctgattt tctaattgtg aaaacattaa
     6721 tcagtattaa agaaatattg actacgtgga tgtaccaata ctagaatttt taattttttt
     6781 cttttgtata gctcttctta cattttggtt taaaaaaaga tggaaaaaga aacctga
//
LOCUS       DASCPJ010000112         8641 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001778719, whole genome shotgun sequence.
ACCESSION   DASCPJ010000112 DASCPJ010000000
VERSION     DASCPJ010000112.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001778719"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="QGF01_05400"
     CDS             <1..575
                     /locus_tag="QGF01_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HJN20354.1"
                     /translation="IAAPPGISPISISPKAKKYMIENPPATMYFNLARYFKYYEESKH
                     TPFTPALPLLYAYREALSILLEEGLENVFNRHKICSDALYSGLSAIGLTPFAKEEDRS
                     ITIVALNYLDGLEDKTFRNTLAEKFRVLVAGGFGNLKGKVFRVGCMGEVQRYHVMRTI
                     SSIASTLDMMGYTVDAKAGLSNAEEKLKAL"
     gene            619..1353
                     /locus_tag="QGF01_05405"
     CDS             619..1353
                     /locus_tag="QGF01_05405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJN20355.1"
                     /translation="MTFNKGSLILVDYTSKVKDTSEVFETTIEEEAKKHSFHDPNIKY
                     QPKLISVGESWVIKGLDEALENTSVGDKTTVEVSSDKGFGERDSGKVRMIPLRKLGED
                     AEKVSIGDTIEVDNKKGIVRFIGSGRIKIDYNHRFAGKTILYDINVLKELKSDEDKIT
                     GILKKNLPVEDEKILFNNKDKILDIIIPQEIFRAENLPIIKHFIQMDLFKFVLGLEKI
                     TFSETYEKPDTKKETKQESKIKDSKK"
     gene            complement(1358..2401)
                     /locus_tag="QGF01_05410"
     CDS             complement(1358..2401)
                     /locus_tag="QGF01_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HJN20356.1"
                     /translation="MRSHKMELPRLIVVGEKNINDIGSFLKSLKNIKKVSLVSGNNVK
                     KIVQSKIESSLSASKIQMVWHLAKTNDKKSIQQIEQNVGKSKSELIVGVGGGRSVDIA
                     KIIGFNLDLPFVSIPTSASHDGIASPFVSIKGNKPHSLVATAPLGVFVDVDIIKKAPK
                     KLLASGCGDLIAKITAVRDWQLGRDKTGEYYGRYSADLALMSAKILMEHSNRFSKKGL
                     DVREVVEALISAGVASCIAGSSRPCSGAEHLFSHAVDKLEPGIGLHGEKCGIGAIMIA
                     KLQGQDWRKIVKTLKSVGAPTTAKEIGLKSEVLAEALTIAQSLRPERYTILKEVNMNE
                     NKAINLAKSTKVL"
     gene            2502..3134
                     /gene="psmB"
                     /locus_tag="QGF01_05415"
     CDS             2502..3134
                     /gene="psmB"
                     /locus_tag="QGF01_05415"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HJN20357.1"
                     /translation="MTTNIEEKILHGTTTVGIKASDGVVLCADMRASAGYFIANNNTM
                     KIQKIYDHVGLTLAGGVADAQNLTDILRYHANIHAIQNNKNIPIKSLARLCSLVFHQN
                     RGYPFMADILLGGYDEEGPSLFNIDMFGSVEQKSYVTTGSGSPVAYGLLEDEYRDDLT
                     VKDAKIVALRAVKAAIVRNIGTGDGINVATIDKNGFQLLTNEQKKSIISL"
     gene            3142..5076
                     /locus_tag="QGF01_05420"
     CDS             3142..5076
                     /locus_tag="QGF01_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HJN20358.1"
                     /translation="MQKKQQQELSNGQNFMATILQSLPKEAVITKINFEGPRIALYTK
                     TPKYLIENNDIIANLVNVIKKRIVIRTDESIRKSEEDVRKILNESVPKDADLKGTFFD
                     TATGEVSIEVKRPWLCQRNKEEFNHAEISEKTGWKLRVRKSTNKASNTIQAINYQLKV
                     SSADRAKHLKGIGEQIFRDRLIQKSEVSLLTLGGFGQVGRSCMLLTTNDSKVLIDCGI
                     NPGARNPYDAFPRLDWANITLDELDAIVIGHAHLDHTGFLPALLKYGYRGPIYCTEPT
                     LPMMNLIQLDAIKVATAQGRIPMYSERDVHQIMRQAITLPYGSVTDISPDIKLVLSNA
                     GHILGSATCHFHIGNGDHNFVYTGDIKYGKSMLLESAFTNYPRVETLLIESTYGLKED
                     IQPTRQEVESAFISAVNTVLKDGGKVLIPIPAVGRAQELMMVIDQYMKSGELTEAPVF
                     MEGMIQEATSIHEAFPEYLARELKKKILETDDNPFDSEYFTNIEHADGREEPLRDGSS
                     CIIIATSGMLEGGPVLEYFKNIAPHKQNKILFVSYQVNGTLGRRVLDGSRQVSILGKE
                     GKVEVITINCDIERLDGFSGHSDYNQLMSFVQRLRPKLRRVLVNHGEKKKSENLSASI
                     RRMYRVASHYPQIQEAIRLF"
     gene            complement(5068..5346)
                     /gene="sepF"
                     /locus_tag="QGF01_05425"
     CDS             complement(5068..5346)
                     /gene="sepF"
                     /locus_tag="QGF01_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HJN20359.1"
                     /translation="MQKQANPTYLKAITIRDPSDIHSIKEDIKKGMILILRVTPLAQK
                     DVEKLRKVVEELYVIARDSNADIARLGEERIIVTPTGVKIWKPDYSLK"
     gene            complement(5417..6427)
                     /locus_tag="QGF01_05430"
     CDS             complement(5417..6427)
                     /locus_tag="QGF01_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HJN20360.1"
                     /translation="MIEKQEQKKVVALLSGGLDSTLAVKTIQKQGFEVSAVAIKTPFC
                     DFDCGRGCGFEIRERAENLGVDLKTVYLGDEYIEMLKNPKHGYGSGMNPCIDCRAMMF
                     EAGKKHMEEIGAEFIISGEVLGQRPMSQFAPALKKIEKLSGLEGKIVRPLSAALLPAT
                     DPEKNGLIKRKDLGMIRGRSRKEQLRMAKDFGIEDPPNAGGGCLLTDPAFSLRAKDLF
                     KHIETPTTNDIDLLKIGRHFRLDENTKLIVGRNENENGMIKSLALPNDILLEAKEHVG
                     PIVMLRGQTGDKYIEFSASVTLRYSDAPKNKIGVVTVHKNEDVEVAIKSAEESSYTKL
                     RI"
     gene            6499..7560
                     /locus_tag="QGF01_05435"
     CDS             6499..7560
                     /locus_tag="QGF01_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HJN20361.1"
                     /translation="MKTRRKILEKQCNSIDCDILVAFEPENLFYMTGFWGEAIGILEK
                     GGKTTIIAPELEAQRAKEDSVDCNIITSQRGYPASILSSILKKEKICTDCQNYTTMQS
                     LKKSIPKIKQSSIPFYNARLIKDSEEIRILKKTSAVIDKMFELCTKTIEKGKRESELQ
                     TTLMSFANANDLFDTGYRSTLNPLIIASGPNSSLPHAQVTKRKFTNGDMITIDLTLRY
                     KGYVSDATRTFGLGSISKEVVTVYEIVKESQKAGLKAVKPGKTCGSVDDACRKIITEY
                     NYGSHFIHSTGHGIGLDVHENPNISGKNKEKMKKNMAITVEPGIYIPKKFGVRIEDSL
                     IVKNRADVMHKFTKDLIII"
     gene            7780..7953
                     /locus_tag="QGF01_05440"
     CDS             7780..7953
                     /locus_tag="QGF01_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-domain-containing protein"
                     /protein_id="HJN20362.1"
                     /translation="MDAKCPECEKVAILDDEITKVKCPHCNFEADYEKYLEIMKEKAL
                     NLASDYIPDRPGF"
     gene            complement(7954..8076)
                     /locus_tag="QGF01_05445"
     CDS             complement(7954..8076)
                     /locus_tag="QGF01_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20363.1"
                     /translation="MPEKILCPRCNEVMSSMQACHLICKNCGAHLDCSDKGNYW"
     gene            8160..>8641
                     /locus_tag="QGF01_05450"
     CDS             8160..>8641
                     /locus_tag="QGF01_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013273.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HJN20364.1"
                     /translation="MANFKLTVSDIKGKSVTKELKDNDVNPLLGLQIGNDADASIVGL
                     EGKMKVTGGSDKSGIPMRPDLHGMARKHILIPKGVGLQDTVKGLRKRKLVRGNTISEE
                     IYQINFRYDGEMKVESPKEEVTEAPKEEPKKEAPKEEPKKEAPKEEPKKEAPKEEPKK
                     "
BASE COUNT         3014 a         1481 c         1480 g         2666 t
ORIGIN      
        1 caattgctgc tccacccgga atttctccaa tatctataag ccctaaagct aaaaaataca
       61 tgattgaaaa tccacctgca acaatgtact ttaacctagc acgatatttc aagtactacg
      121 aagaatctaa acatactcca tttactcctg cattaccatt actttatgca tatagagaag
      181 cattatcaat tttattagaa gaaggattag aaaatgtctt taatcgtcac aagatttgtt
      241 ctgatgcatt atactctgga ctttcagcaa tcggtttgac accatttgca aaagaagaag
      301 atcgttcaat aacgattgta gctttgaatt atcttgatgg tctagaggat aaaacatttc
      361 gaaatactct agcagagaaa ttcagagttc ttgtagcagg agggtttgga aatctcaaag
      421 gaaaagtttt tcgagttgga tgtatgggag aagttcaacg atatcatgtc atgagaacta
      481 tttcttctat tgcgtctaca ttagatatga tgggttatac tgttgatgca aaagcagggc
      541 ttagcaacgc tgaagaaaaa ttaaaagctc tttaatccac gttaacttat ataaggaaat
      601 tcaaaaacgc agaatacaat gactttcaat aaaggctcat taattttggt cgattatact
      661 tctaaagtaa aggatacctc ggaagtattt gaaacaacca ttgaggaaga ggcaaaaaaa
      721 cattcttttc atgatcctaa cataaaatat caaccaaaac ttatttcggt tggagagtca
      781 tgggtaatca aaggattaga tgaagcacta gaaaatactt cagttggaga caagacaacc
      841 gttgaagttt catctgataa aggatttggt gaacgagatt ctggcaaagt tagaatgatt
      901 ccactgcgta aacttggaga agatgccgaa aaagtttcca tcggagatac gattgaggta
      961 gataacaaaa aaggaattgt aagatttatc ggttctggca gaattaaaat tgattataac
     1021 cacagatttg caggaaaaac aattctatat gacattaatg ttctcaaaga attaaaatct
     1081 gatgaagaca aaataacggg aattttaaag aaaaaccttc ctgtagaaga cgaaaaaatc
     1141 cttttcaata ataaggataa aattcttgat atcataatac cacaggaaat atttcgtgct
     1201 gaaaatttac ctataataaa acactttata caaatggatt tattcaaatt tgttcttgga
     1261 ttagaaaaaa ttaccttttc agagacatat gaaaaaccag ataccaagaa agaaacaaaa
     1321 caagaatcaa aaataaagga tagtaaaaaa tagaaaatta tagtactttt gtacttttag
     1381 caagattgat tgctttattt tcattcatat tgacttcttt taaaatagta tatctttctg
     1441 gtcttaagga ctgtgcaatg gtcaatgctt ctgcaagaac ttcagatttt aaaccaattt
     1501 cttttgcagt tgtaggagca cctacacttt tcaacgtctt aacaattttt ctccaatctt
     1561 gaccttgtaa ttttgcaatc ataattgcac caataccaca tttttcaccg tgaagtccaa
     1621 ttccaggctc taatttatca actgcatggg aaaacaaatg ttctgcacca gaacaaggtc
     1681 tactactacc agctatgcac gatgcaacac ctgcactaat taatgcctca acaacctctc
     1741 tgacatccaa cccttttttt gaaaatctat tagaatgctc catcaaaatt tttgcactca
     1801 ttaatgctag atctgcagaa tatcttccat aatactcccc agtcttatca cgtccaagct
     1861 gccagtctcg tacagctgta atttttgcta taagatcgcc acatccactc gcaagtagtt
     1921 tttttggtgc ttttttgata atatctacat ccacaaaaac tccaagtggt gcagtagcta
     1981 ccagcgagtg tggtttattg cctttgatag aaacaaaagg actagcaata ccatcatgtg
     2041 acgcagatgt tggtatgctg acaaatggca aatcaagatt aaatcctatt attttagcaa
     2101 tatcaacaga acgaccacca ccaacaccca caattaattc acttttactt ttacctacat
     2161 tttgctctat ttgctgaatg gattttttat cattagtttt agctaaatgc caaaccattt
     2221 gaattttgga tgcagaaaga gaactttcaa tcttgctttg aactatcttt ttaacattgt
     2281 ttcctgatac caaagaaact ttttttatat tttttaatga ttttagaaaa cttcctatat
     2341 cattgatatt tttctctccg acaacaatca gtcgtggtaa ttccatctta tgtgatcgca
     2401 tagatttttt cgttttttgc actttttatc atttgtctac aaacaaaaag ttatttaaaa
     2461 gggtgcaaat aactcgaaat atctcattag aggtattatt tatgacgaca aacattgaag
     2521 aaaaaatatt acatggcaca accactgtag gaatcaaggc atcagatggt gttgttttat
     2581 gtgctgatat gagggcaagt gctggatatt tcattgccaa taataataca atgaaaattc
     2641 aaaaaatcta cgatcatgtt ggtttgacat tagcaggagg agttgcagat gcacaaaatc
     2701 taacggatat tttgagatat catgcaaaca tacacgctat tcaaaataat aaaaacattc
     2761 caataaaatc attagcaaga ctttgttctc tcgttttcca tcaaaataga ggctacccat
     2821 ttatggctga cattttgtta gggggttatg atgaagaagg accatcactt ttcaatattg
     2881 atatgtttgg ttctgtagaa caaaaatcct atgtaacaac tggaagtggt tcacctgttg
     2941 catatggtct tttagaagat gaataccgtg acgatcttac agttaaagat gctaagatag
     3001 tagctcttcg cgcagttaag gctgcaattg taagaaatat tggaactggt gatggaatca
     3061 atgtagcaac aattgataag aacggattcc aacttttgac caacgaacaa aaaaaatcta
     3121 ttatctcact ttagtgatta aatgcaaaag aaacagcaac aggaattatc taacggccaa
     3181 aatttcatgg caaccatact gcaaagttta cccaaagaag cggtaataac aaaaattaat
     3241 tttgaaggtc ctagaattgc actttacaca aaaactccaa aatatttgat agaaaataat
     3301 gatataattg caaatctagt taatgtgata aaaaaacgaa ttgttatcag aacagatgaa
     3361 tcaataagga aaagtgaaga agatgttaga aaaattctta atgaaagtgt accaaaagat
     3421 gcagatttaa aaggaacatt ttttgatact gctactggag aagtttctat agaagtaaaa
     3481 cgtccttggc tttgtcaaag aaataaagaa gaatttaacc atgctgaaat atctgaaaaa
     3541 actggctgga aattacgtgt gagaaaatct acaaacaagg catcaaacac aattcaagct
     3601 ataaattatc aactcaaagt ttcttcagct gatagagcaa aacatctgaa gggaatagga
     3661 gaacaaattt ttagagatcg tctaatacag aaatctgaag tatcattact gacattaggt
     3721 ggttttgggc aggtaggaag atcatgtatg ttgttaacca caaatgatag taaagtattg
     3781 atcgattgtg gaatcaatcc tggcgcacgt aatccatatg atgcctttcc taggctcgat
     3841 tgggctaaca taacacttga tgaactggat gctattgtga taggccatgc acatcttgat
     3901 catacaggat ttttgcctgc attactgaaa tatggttatc gtgggccgat ttattgtaca
     3961 gagcctacat tgcccatgat gaatctgatt caacttgatg caatcaaggt tgcgactgca
     4021 caaggaagaa tacccatgta ttccgaaaga gatgtgcatc aaataatgcg acaggcaatt
     4081 actctacctt atggaagtgt aactgatata tctcctgata tcaaactagt cctatctaat
     4141 gctggacata ttctaggctc agctacttgc cattttcata ttggtaacgg tgatcacaat
     4201 tttgtataca caggtgatat caaatatggt aaaagtatgc ttctcgaaag cgctttcaca
     4261 aattatccac gagtagaaac tcttcttatc gaaagtacat atggtttgaa ggaagatata
     4321 caacctacaa gacaggaagt ggaatctgcg tttatttcag cagtaaatac ggttttaaaa
     4381 gatggaggaa aagtactcat tccaattcct gcagttggta gggcacaaga attaatgatg
     4441 gttatagatc aatatatgaa atctggtgaa ttaacagaag ctccagtatt catggaagga
     4501 atgattcagg aagctacttc aatccacgaa gcatttccag aatatcttgc tagagaacta
     4561 aaaaagaaga ttctagaaac agatgataac ccatttgatt cagaatattt tacaaatata
     4621 gaacatgctg atggtagaga agaaccattg agagatggat cgtcttgtat tataatagca
     4681 acttctggta tgctagaggg cggtccggtt cttgaatatt tcaagaatat tgctcctcat
     4741 aaacagaata aaatattatt tgtttcctat caagttaatg gtacgcttgg acgaagagtg
     4801 ttagatggat caagacaggt atccatattg ggaaaagaag gaaaggttga agttattact
     4861 ataaattgtg atatagaaag acttgatgga tttagtggac acagtgatta caaccaactt
     4921 atgtcatttg ttcaaagact gagacctaaa ctaagacgcg tacttgtgaa tcacggagaa
     4981 aagaaaaaat ctgaaaatct gtctgcatca atacgtcgta tgtatagagt tgcatcacat
     5041 tatccacaaa tacaagaagc aataagatta ttttagacta taatcaggct tccaaatttt
     5101 tacacctgtt ggtgtcacta taatcctctc ttcaccaaga cgagcaatat cagcattcga
     5161 atctcttgca attacataaa gttcttctac aacttttctg agtttttcaa catctttttg
     5221 tgctaatggt gtaaccctaa gaattagaat catacctttt ttgatatcct ctttaattga
     5281 gtgtatatca ctaggatctc taattgttat cgccttgaga tatgttggat ttgcctgttt
     5341 ttgcattcag ctattttgtc accaactccc ccaaaaagtc tttctaattt tttacgagta
     5401 aacaactatc tgttatctaa attctgagtt ttgtatatga ggattcttcg gcagatttta
     5461 ttgctacttc tacatcttca tttttgtgaa cagtgacaac acctatcttg ttttttggtg
     5521 catcagaata gcgtaatgta acactagctg aaaattctat atatttgtct ccagtttgtc
     5581 ctcttaacat tacaatcggt ccaacatgtt ccttagcttc aagtaagata tcattaggta
     5641 atgcaagtga tttgatcatt ccattttcat tttcatttct tccaacaatt agctttgtat
     5701 tttcatccaa tctgaaatgt cttccaatct ttaacaggtc aatatcgttt gttgttggtg
     5761 tttcaatatg tttgaacaag tccttagctc ttaatgagaa cgcaggatct gtcaagagac
     5821 aacctccacc agcgtttggt ggatcctcaa taccaaaatc ttttgccatt ctaagttgtt
     5881 cttttctgga acgacctctg atcataccaa gatcctttct ttttatcaaa ccattctttt
     5941 ctggatcagt ggcaggtaac aatgctgctg ataacggtct aacaattttt ccctctaaac
     6001 ctgataactt ttcaattttt ttcaatgcag gtgcgaactg actcattggt ctttgtccaa
     6061 gtacttcacc tgaaataata aactccgcgc caatctcttc catgtgcttc ttaccagctt
     6121 caaacatcat tgctctacaa tcaatacatg gattcattcc agatccataa ccgtgttttg
     6181 gattttttaa catctcaata tattcatctc caagataaac tgtttttaga tcaactccta
     6241 gattctctgc tctttctcta atctcaaaac cacaacctct accacaatca aaatcacaaa
     6301 atggtgtctt gatcgccact gctgaaactt caaatccctg tttttgtatt gtttttacag
     6361 caagtgtact atctaatccg ccggaaagca gtgcaacaac tttcttttgt tcttgctttt
     6421 ctatcataaa actagcaagg aatctggata tacaaacttt acaacataca taaattcaca
     6481 aaaacatttc tttgaacgat gaagacacgc agaaaaatat tggaaaaaca atgtaattct
     6541 attgattgtg acattcttgt cgcatttgag cctgaaaacc tcttctacat gacaggattc
     6601 tggggtgagg caattggtat attagaaaag ggtggaaaga ctacaattat tgctcctgaa
     6661 ttagaggcac aaagggcaaa agaagattca gttgactgta atattataac ttcacaaaga
     6721 ggttatcccg catctatatt atctagtatt ctgaagaagg aaaaaatctg tactgattgt
     6781 cagaactaca caacaatgca atcattaaaa aaatcaattc caaaaataaa acaatcttct
     6841 attccttttt acaacgcacg tctaatcaaa gactctgaag aaatacgtat actgaaaaaa
     6901 acatctgctg taatagataa aatgtttgaa ctatgtacaa aaaccatcga aaaaggcaaa
     6961 agagaatctg aactgcagac aactttgatg tcatttgcaa acgcaaatga cttatttgat
     7021 actggttacc gttcaacctt aaacccattg ataatagcta gtggtccaaa ttcttcactt
     7081 ccgcatgcac aagttaccaa aaggaaattt acaaacggtg atatgattac gatagatttg
     7141 actttgagat ataagggata tgtttctgat gcaactagaa catttggact tggctcaatt
     7201 tctaaggagg tagtaactgt ctatgaaatt gtaaaggaat ctcaaaaggc tggattaaaa
     7261 gctgtaaaac ccggaaagac ttgtggatcc gtggatgatg catgcagaaa aattataaca
     7321 gaatataact atgggtcaca cttcattcat tccacaggtc atggaatagg attagatgtt
     7381 catgaaaatc ctaacatttc tggtaaaaat aaggaaaaga tgaaaaaaaa tatggctatt
     7441 accgttgaac ctggaatcta tataccgaaa aaatttggtg ttcgcataga agattcgtta
     7501 atagtgaaga atagggcaga tgttatgcac aagtttacga aagacttgat tataatttga
     7561 aaacacttcc gattatcatg atagtataac taattttatt atggtgaatt tcactttgtc
     7621 tttttccccc ttcgcaaaag ttccatatta tgagttcact acgtttctga taaatctgat
     7681 cagaggcttg gaaagctgac gtcctgccgg gctggactaa ctccgccaat ttgaattttg
     7741 aaaattagtg ttatatagct tcatgagaaa ttatcattta tggatgcaaa atgtccggaa
     7801 tgtgaaaaag ttgcaattct tgatgacgag attacaaaag taaaatgtcc acactgtaat
     7861 tttgaagctg attatgaaaa atacttggaa attatgaagg aaaaagcatt gaatcttgct
     7921 tcagattata ttccagatcg accaggattt taactaccaa taattgccct tatcagaaca
     7981 atctaaatgt gcaccacagt ttttacagat tagatgacat gcctgcatag atgacataac
     8041 ttcattacat cttgggcaca atattttttc tggcacaaac acactactcg ttttatgttt
     8101 taagattttg gcaattaagt ccaaaggtta attagtcgtt tgactatttt tttgaatact
     8161 tggctaattt caaattaaca gtatctgata ttaaaggaaa gtcagttaca aaggaattaa
     8221 aagataatga tgtcaatccg ttacttggtc tgcaaatagg taatgatgcg gatgcctcta
     8281 tcgtaggatt agaaggaaag atgaaagtaa caggtggaag tgataaatca ggtattccta
     8341 tgcgacctga tttacatggt atggcaagaa aacacatctt aataccaaaa ggagttggtt
     8401 tgcaagatac tgtaaaagga ttaagaaaac gaaagttggt tagaggaaat actatttctg
     8461 aagaaattta tcaaattaat tttagatatg atggtgaaat gaaagtagag tcaccaaagg
     8521 aagaagtcac ggaagctcct aaagaagaac caaagaaaga agctcctaaa gaagaaccaa
     8581 agaaagaagc tcctaaagaa gaaccaaaga aagaagctcc taaagaagaa ccaaagaaag
     8641 a
//
LOCUS       DASCPJ010000113        15309 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001782195, whole genome shotgun sequence.
ACCESSION   DASCPJ010000113 DASCPJ010000000
VERSION     DASCPJ010000113.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 15309)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15309)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15309
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001782195"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(44..1021)
                     /gene="hisG"
                     /locus_tag="QGF01_05455"
     CDS             complement(44..1021)
                     /gene="hisG"
                     /locus_tag="QGF01_05455"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HJN20365.1"
                     /translation="MAPVKFSIPKGSLEEATFKLLEESWTKVRRRPRTYRVFLDDPDI
                     QVKMLRPQEIPTLVGDGLYDVGITGQDWVDENKADVEKLLDLEYGKIKLVIAIPDSYK
                     YKSLDEMILAYGKKKKTLRISSEYLTNASKFIKKCKSYKKLYGSKDPQIVTPWLRLGT
                     NKNVQIHLSFGATEAKPPEDVDAIMDVTETGTTLKQNQLKIVDTVMQSSAHLIANKKS
                     LKDKKKRQKIFDIVTLMRGAVQGRKYLHIYMNVKEKNLKKLLTEITSLKRPTVSPLSE
                     KGWYGVNTVIPKSEFHKLVPKLRKIAQGLVVHEPRQILELEEIKRDEEN"
     gene            complement(1062..2222)
                     /locus_tag="QGF01_05460"
     CDS             complement(1062..2222)
                     /locus_tag="QGF01_05460"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HJN20366.1"
                     /translation="MRDSSISKFFEKTLKERLGVVADFSQLSQDELKIIEDATGGISF
                     EKADNMIENAIGTFSLPLGIATNFRINGKDYLIPMVIEEPSVIAASSKAAKTARIHDG
                     FKAEADESYSIGQIQVVDTDVQAAIPKIIGASHDILNLANEQSNTLSKLGKGAKEISC
                     KDIETDSGHMLIVELLIDVGDAMGANVTNTMCEAVAPMIEKLSGGKTLLRILSNYSTK
                     RMVRASAVFDKDEIGGEGVVDDMILAFQFADNDTYRAVTHNKGVMYGVIAVANATGQD
                     SRAIEAAAHAYASKTGSYRSLTKWEKDGNGNLVGNFELPLSVGIVGGVIQHHPIAKIC
                     TKILGVSTAQELSCVIAVVGLAQNFSAMRALATEGIQKGHMKLHARKEKQNS"
     gene            complement(2224..3318)
                     /locus_tag="QGF01_05465"
     CDS             complement(2224..3318)
                     /locus_tag="QGF01_05465"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HJN20367.1"
                     /translation="MDKRLRIAGIVAALIFAVIIWVSPSNPPPIPAPVTSFSNEFVEI
                     IATDLEKPWSIGFGDDKIFLTEKDGRVRIIESDTLLDDPLITLRTANVPDGGLLGLAV
                     HPNFSENHFLYLYYTYEEDGILWNKILRVSESQNKIVETKTILDKIPGSMFVNGGILK
                     FGPDEKLYVGTGSISDSSHGSQDLKSLEGKILRLNDDGTIPDDNPFSNSPVFSYGHRD
                     PKGMAWDKDGNLFMTEIGPSTNDEINLIHAGKNYGWPEHECIGSGKFIAALNCYDPGI
                     EPGGIIFYYGDKLDVKKSLLMATLKGSHLFSLEIDENDLESQTIILSGVGRIRDVVQG
                     PDGYIYLITSNTDGKGFPDGNDDKLLRLLK"
     gene            3397..3471
                     /locus_tag="QGF01_05470"
     tRNA            3397..3471
                     /locus_tag="QGF01_05470"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3432..3434,aa:Met,seq:cat)
     gene            complement(3469..4617)
                     /locus_tag="QGF01_05475"
     CDS             complement(3469..4617)
                     /locus_tag="QGF01_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20368.1"
                     /translation="MGIGTQSTFGMQTEYTFDKYGDASFEPIPEWIKNNAGWWAEGKI
                     FDASFVAGIEWLITNGIITVSTTEQEAVAGYEIPKWIKNNAGWWAEGKIPDSEFISGL
                     EWLIEHGIIMVNLPEDVDPYDITFAPILSNVSQQNLQYVTTTFFHVFGDLDSIQTIDE
                     VKHWGAVYLGLNEDRMDEYNEVSIWNDTQKAAVVFPTFTSAAYGEPGFYTYYRGECDE
                     CTTTNIKIPRLFFPSSGNAIQSFSLMGYKVLDDIEIDKNPSILEKYDKIIMLHNEYVT
                     QTIFDAITNHPNVIYLYPNALYAEIEVDYEANLITLIRGHGYPDAEISNGFDWEFDNT
                     HPYEYDTECSNMEFYRIDNGWMTNCYPEQVFTNSVNGTHKILKLIKEL"
     gene            complement(4783..6105)
                     /locus_tag="QGF01_05480"
     CDS             complement(4783..6105)
                     /locus_tag="QGF01_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HJN20369.1"
                     /translation="MKNSTVIHIGIDDTDSPKGMCTTFLAYKIVKFLEKNKVQFVDYP
                     SLIRFNPNIPWKTRGNGAVRLSIKTNNPKKIKNKITKFVDDYSETKNGANPGLVFYEN
                     EIVTHSFHKFSELALWKLISREKAKQFILENKIDSFYLGNGQGLVGAIGAIGYKFSDH
                     TFELLSYRKKSQFGKKRIIAKDSVKNMQSITFPETFNSYDIESDRALITPHGPDPVFY
                     GIRGENAESVVTASKIVKANEKLDGYMVFKSNQGTSDHLKNELDVNDLRPYTSGFLAG
                     QVCSSPIMAQGGHVFFSIRVKDKKIKCGIYKPTKITTIAQNLILNDKIRIGGGIRKAS
                     KNHERIVNVEFLDVMELAKDIRASNPSCKKCSKKMKSKGSKQGFECTKCGSKSSSKIT
                     SEIPRKIQCQLYLPVMSAHRHLTRPYQRLKKRNKQTRFDTSTPWFCVY"
     gene            6191..7186
                     /gene="dph2"
                     /locus_tag="QGF01_05485"
     CDS             6191..7186
                     /gene="dph2"
                     /locus_tag="QGF01_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HJN20370.1"
                     /translation="MIIIDERKIFDVIEAKKPVSVALNGPDGILPKVQDLALKINQKC
                     GIPAYLLADATWGSCDLNSIGANVLQAEILFNIGHTNKLEIFEKNIYMIDAYDDIPFD
                     NVVEKCVKQFKGNTVSLLTDSQYLHQIDHIAKTLENNGVIVKIGNGKGQLNDGQVFGC
                     EFYPATEIKNDVDANIFLGQSNFHAAGIALATNKPTYILDPYFNEVREVTEFAKKMQK
                     RATLEIYKAADAETFGVIVGLKEGQLSKLTALKFKKELEDCGKTVHLIALTDITNERL
                     QNLKDIDAFIQVACPRISTDNKFDKPVLSTPQALSLLKVLRKEGIGEYFERNHWL"
     gene            7219..7767
                     /locus_tag="QGF01_05490"
     CDS             7219..7767
                     /locus_tag="QGF01_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HJN20371.1"
                     /translation="MSDTVLPGDKIALIEECEAGKNSYDDDGLIRSMIVGKSEMNKKE
                     RLIDVENQKVLSVPKTDDIIIGTIEMVTPSMIAVLINYINDKPVKSQVECICSIRNLR
                     KRSPALIKDIVKARVIGTLNGVIHATIQDKELGVLFTKCRKCGQDVVLFRDIVKCIEC
                     GWTDDRKISTDFLQSNFLKMRE"
     gene            7771..8610
                     /gene="pheA"
                     /locus_tag="QGF01_05495"
     CDS             7771..8610
                     /gene="pheA"
                     /locus_tag="QGF01_05495"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HJN20372.1"
                     /translation="MTRVSFQGERGAYSEAASISFFDNTIDTIPCTTFADALKNTEND
                     MSDYSVLPVENSLEGSVGESNDLLVTVNLDVVGEIYHRIRHCLIGTGSLENIDTVYSH
                     TQALGQCRKFIQENSLKTVPSYDTAGSVKIIKDLNKNNVACIASKNAAEIFGIPVIKQ
                     GIEDNRNNYTRFLVLSKESNEDSLMSKQNDSSKKKTSIIFSVKHEAGALYQIIKEFAT
                     YKINLTKIESRPNKNTAWEYNFYVDFEGSQDDSMIKEMLQKIKGNSSFLKILGTYPMT
                     NLD"
     gene            complement(8607..9077)
                     /locus_tag="QGF01_05500"
     CDS             complement(8607..9077)
                     /locus_tag="QGF01_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20373.1"
                     /translation="MLSKYTIIGLIVGCVICGLGIASLVDSLAYPNEIRETNDTFGVG
                     DSDRIRFNAPADSVQMLEITGDTFDVKIFTPNEKNNVDNSYKGKATFSWTNTDSGENL
                     IQIQNTGKSEFNISGSFELSRDPLFFTYHILVIIAGIVVIGFSAAFSARKPRGF"
     gene            complement(9112..10548)
                     /gene="guaB"
                     /locus_tag="QGF01_05505"
     CDS             complement(9112..10548)
                     /gene="guaB"
                     /locus_tag="QGF01_05505"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HJN20374.1"
                     /translation="MSIREGLTFDDVLLVPKFSDIVSRSQTDLTTQLSRNISINIPMI
                     SANMDTVTESTMAVTIAREGGIGIIHRFLTIQEEVDEIHKVKRAGSVIIENPYLINPE
                     QTIQDAFKIMNEKQVSGLLVTDSSSKLIGILTERDVLFEPVDCSKLVKDLMTKDVVSA
                     KPGIDFQQAKEILKNNRIEKLPLTDENNQIKGLITSQDISNLEKYPNASKDEKGRPLV
                     GAAVGVKGDFMQRTEALLDAGVDVIVVDIAHGHSENAINTVKNIKKAFPNCELIAGNV
                     ATAHGTEDLIKAGVDAVKVGVGSGSICITRVITGSGVPQLTAVLDCAKIGNEHNIPII
                     SDGGTRTSGDATKALAAGAHSVMIGGILGGTDESPGSTISKNGKRFKIYRGMASLGAS
                     LGRKSKDSGNAGLTDDLNDYVAEGVEGMVPYRGSVTDIITQMTGGIRSGLSYCGAHNI
                     QQMQKNAEFIKISRAGFAESQPHDVDVM"
     gene            complement(10597..11460)
                     /gene="folP"
                     /locus_tag="QGF01_05510"
     CDS             complement(10597..11460)
                     /gene="folP"
                     /locus_tag="QGF01_05510"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HJN20375.1"
                     /translation="MINCVNKLGTISVGSSNPVRIMGILNTSPESFYKKSISSSKQKI
                     IDTVNRMQEEGADFIDIGGMSTAPYLSTMITEKMEINRIIKGIKIVQQHSNLPISVDT
                     CRSSVAKEALELGVDIINDITGLKYDPLMPKIVEKYSPSLILCAYSKKTITGNQVQKT
                     KNLLQESLNISKSANISQSQIVLDPSIGFFRNKGKKSAIFTKINSDSIQRDLTILQNL
                     RAIRLNKPILVSVSNKSFLGKILEKENPSDRLYGSLAAEVISVLNGAHIIRTHNVSET
                     KDAVIIAQRLS"
     gene            11483..12223
                     /locus_tag="QGF01_05515"
     CDS             11483..12223
                     /locus_tag="QGF01_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HJN20376.1"
                     /translation="MTIVGWESRYREILKEFGYSRNNDNQSCRLLDSILPKKVDLVKI
                     RCLIENKPVFIVGAGPSLPSSIPILKKYKKITKIVADGATQAIIENGLKPDIVVTDLD
                     GDSKSLKKAGRTNTMMVVHAHGDNSEKLGFAKNFKNCIGTTQAKPTTNIHNFGGFTDG
                     DRCVFLANHFKAKKIILLGMDFGTKIGKYSKRTISNRRIKIAKLRRGKKLLEWLAPKS
                     KSDLYSTTKINGFTKVNLRSIDNIIAKN"
     gene            12233..13591
                     /locus_tag="QGF01_05520"
     CDS             12233..13591
                     /locus_tag="QGF01_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJN20377.1"
                     /translation="MKIIQLNSASNLIEDSIDGFRVKILCDPWLNGEEYLGSWAMYPP
                     YNFKPENFSDLDFIYISHIHPDHSSASTLSKLDKKIPVLIHNFPEKFLKNKIENLGFK
                     TIQLEHGVRTRLKNNLHVNIMAADNCDPNVCGKIMGCGLSEVRFQTTQIDTMAVFDNG
                     NQVIVNTNDCPFDIAKNTASIIKSIYHKIDVLLLGYVAASSWPHSYILPEVEKEEAAK
                     KKQDVKLQTAKKYIELLQPKYYIPFAGRYTLCGKNTVLNPYRGEPELEDAFEWLHNNI
                     EQENHRGIILNDDCWFDVDTGCTSKNYVPINKNEKEDYVNNILSRKKFPYETQPIPTT
                     AEILDLIPKAYENFDKIRQKIGWISDTTVILKTTNDDLVIAISCQGKGFKKISNDDVK
                     KYEQCMIISLDARLLKWLLHGPYKAHWSDADLGSHLRYERIGSIYKRGLFYCWNHFSA
                     NV"
     gene            13631..14167
                     /locus_tag="QGF01_05525"
     CDS             13631..14167
                     /locus_tag="QGF01_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20378.1"
                     /translation="MGLSDEQIRDMLELKEDLEEKIIKYKEQIEKLEKNISVLDSILK
                     QSSFAKASDLTVDTKKQAVSIPITKNSDGTTIANVFVSDDKVSIVLEDNVTINPETPP
                     LKTFFVDRIIGEMKKKDNQEAENGTINKDDIIDCMIKNNGSALREIIIKNYRQKERVD
                     EIITTTTWSLRKMLENSE"
     gene            14172..>15309
                     /gene="guaA"
                     /locus_tag="QGF01_05530"
     CDS             14172..>15309
                     /gene="guaA"
                     /locus_tag="QGF01_05530"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HJN20379.1"
                     /translation="MDKIVVLDFGSQYSHLICRRIRDFSVYAELVPYNISLEELKKLN
                     PKGIIFSGGPSSVYDSNAPMPNKEIFQLQLPILGICYGHQIIVNNFGGQVKRAKKEYG
                     SSILSVDNNSDILSGIGDSIRAWMSHGDEAENVPKGFEVIGHTENSQSAVITNNERTV
                     YGIQFHPEVVHTEKGIEILKNFVLKICDAKQDWSMEQFVENSIENISKLDGNVLCGVS
                     GGIDSTVTALLIHKAIGDKLKCIFVDNGLLRLNEVEEIENMFVNNFKINFVKIDAGKQ
                     FLSELNGVVDPEAKRKIVGEEFVKIFTKFAKDNGPFTWLAQGTLYPDVIESGVSKGPA
                     SVIKTHHNVGGLPDWLELKVLEPLRDLYKDEVRKVAKILDVPENL"
BASE COUNT         5026 a         2575 c         2565 g         5143 t
ORIGIN      
        1 gattctacaa agctgtcaat attacgaacc tgtaatatct tgatcaattc tcttcgtcac
       61 gttttatctc ctcaagttcc agtatttgtc ttggctcgtg aactaccaat ccctgtgcaa
      121 tctttcttag tttaggaact aatttgtgaa attctgactt ggggattacg gtgtttacac
      181 cataccatcc tttctcactg agtgggctga cggtaggtct cttaagagag gtaatttctg
      241 ttagcagttt tttcagattc ttttccttca cattcatgta aatgtgtaag tactttctgc
      301 cctgaactgc acctctcatt agtgtaacaa tatcaaatat tttttgacgt tttttcttat
      361 cctttagaga tttcttgttg gcaatcaagt gtgcgcttga ctgcattact gtatctacta
      421 tcttcaattg attttgtttc aaagttgttc ctgtctcggt aacatccatt atagcatcaa
      481 catcttccgg tggtttggct tctgttgcac caaaagatag gtgaatctga acatttttgt
      541 tagttcccaa gcgtagccaa ggagtaacaa tttgaggatc ttttgaaccg tagagttttt
      601 tgtatgattt acatttttta ataaactttg aagcgtttgt aagatactct gatgaaattc
      661 taagtgtctt tttcttcttt ccataagcca aaatcatctc gtcaagtgac ttgtacttgt
      721 aactgtcagg aatggcaatt acgagtttaa tttttccata ttctaaatca agtagtttct
      781 caacatctgc cttattctca tcaacccaat cctgtcctgt gatacctaca tcatacagac
      841 cgtctccgac cagggttgga atctcttgag gacgaagcat tttgacttga atatctggat
      901 catccaagaa tacacgataa gttcttggtc ttcgcctgac ttttgtccat gattcttcta
      961 gtagcttgaa cgtagcctct tctaggcttc ctttaggaat tgaaaatttt actggtgcaa
     1021 tttttttcat aatatcttaa ggttatataa atgcgatctg tttatgaatt ttgtttttcc
     1081 tttcttgcat gtaatttcat gtgacctttt tgaattcctt ctgtcgctaa agcacgcatg
     1141 gcagaaaagt tttgagccaa gcctactaca gcaataacac atgaaagctc ttgagccgta
     1201 gagacaccaa gtatctttgt acagattttt gcgattggat ggtgctgaat aaccccacca
     1261 actatgccta cagagagtgg taattcaaaa tttccaacca agtttccgtt gccatccttt
     1321 tcccatttgg ttaaagagcg ataagatccg gtttttgatg catatgcatg agcagcggct
     1381 tcaatagccc tactatcttg tcctgttgcg ttagctacag caattactcc gtacattaca
     1441 cctttgttgt gagttactgc tctgtatgta tcattatcgg caaattgaaa agctaagatc
     1501 atgtcatcaa caactccttc cccaccaatc tcatctttat caaatacagc agaagcacga
     1561 accattcttt ttgtggaata attagaaaga atacggagta gagtttttcc gccagataat
     1621 ttctctatca ttggtgccac agcttcacac atcgtatttg ttacatttgc acccatagca
     1681 tctcctacat caatcaataa ttcaacaatt aacatgtgac cagaatctgt ctctatgtct
     1741 ttacatgaaa tttcttttgc accctttcct agtttagata atgtattact ttgttcattg
     1801 gcaagattta ggatgtcatg ggaagcgcca atgattttgg gtattgctgc ttgaacatct
     1861 gtatcaacca cttgaatttg tccaatactg tatgattcat cagcttctgc cttgaaccca
     1921 tcatgaatcc tagcagtttt cgctgcttta gaagaagctg caatcactga tggttcctca
     1981 atcaccattg ggataagata gtctttaccg ttaattctaa aatttgttgc aattcctaat
     2041 gggagtgaga atgttcctat tgcattttct atcatgttat cagctttttc aaaagaaatt
     2101 cctccggtgg catcttcaat aatttttaat tcgtcttgtg ataactgaga aaaatctgcc
     2161 acaacgccaa gtctttcttt tagggttttt tcaaaaaatt ttgatatgga tgaatctctc
     2221 attttatttt aatagcctca ataacttatc atcattacca tcaggaaaac cttttccatc
     2281 cgtattagaa gttattaaat agatatagcc gtctggaccc tgaaccacat ctctaatcct
     2341 ccctactcca ctaagtatta ttgtctgaga ttctagatca ttttcatcaa tttcaaggct
     2401 aaatagatgt gaacccttca aggtagccat tagaagagat tttttcacgt caagtttatc
     2461 tccgtaataa aatatgattc cgcctggctc gatgccagga tcatagcaat ttagtgcggc
     2521 aataaatttt ccacttccta tgcattcatg ttcaggccaa ccataatttt ttcctgcatg
     2581 gattaaattg atttcatcgt ttgttgaagg gccgatttct gtcatgaaaa gatttccatc
     2641 tttgtcccaa gccatacctt ttggatctcg atgaccatat gaaaatactg gagaattaga
     2701 aaatgggtta tcgtcaggta ttgttccatc atcgtttaat ctgagaattt ttccctccaa
     2761 tgatttcaga tcttgagaac cgtgggagga atctgatatg gaaccagtac ctacatagag
     2821 tttttcatct ggaccaaatt tcagtatgcc accgttgaca aacatagaac caggaatttt
     2881 atcaaggatt gtttttgttt caacaatctt attttgtgat tcagaaactc tgagaatttt
     2941 attccatagt ataccatctt cctcatatgt ataatagaga taaagaaaat gattttcgct
     3001 aaaatttggg tgtactgcaa gtcctaataa tccaccatca ggtacattcg cagttcttaa
     3061 tgtaattaat ggatcgtcaa gtaaagtatc agattcaatg attctaacac gtccatcttt
     3121 ttctgtgaga aatatcttat catcaccaaa accaatgctc caaggttttt ctaaatccgt
     3181 tgctataatt tctacaaatt cattgctgaa gcttgtcaca ggtgctggta ttggtggagg
     3241 attagatggg gaaacccata ttatcacagc aaaaattaat gcggctacaa tcccagcaat
     3301 tctaagtcgt ttatccataa gaaactgaat ctgtgaatcc taataattat tccgagtttg
     3361 ataaaggcgt atatagcgca taaagaattt ttgttcagcg gggtggggaa gtcaggtcat
     3421 cccgtcgggc tcataacccg aaaatcagta gttcaaatct actccccgct ataattcttt
     3481 gattagtttt aatattttgt gtgttccatt aacagaatta gtgaatacct gttccggata
     3541 acagtttgtc atccaaccgt tatcaatcct gtagaattcc atgttagaac attcagtgtc
     3601 atattcatat ggatgtgtat tatcaaattc ccaatcaaac ccattggata tttcagcgtc
     3661 tggatatcca tggcctcgga tgagtgttat gagattggcc tcataatcaa cttcaatttc
     3721 tgcataaagt gcattggggt aaagataaat cacattggga tgatttgtta ttgcatcaaa
     3781 tattgtttgt gtcacatatt cattatgtaa catgattatt ttgtcgtatt tttctagtat
     3841 acttggattt ttatcaatct caatatcatc tagaacctta taacccatta atgagaaaga
     3901 ttgaattgca ttaccacttg atggaaaaaa tagtctagga atttttatgt tagttgttgt
     3961 acattcatca cattctcccc tatagtatgt ataaaaaccc ggctctccat acgcagcact
     4021 ggtaaaagtt ggaaatacta ccgctgcttt ttgagtatcg ttccatattg acacttcgtt
     4081 gtattcatcc attcgatcct cattgagacc tagatagact gcaccccaat gctttacttc
     4141 atcaatagtt tgtatcgaat ctaaatcccc aaagacgtga aagaatgtgg tagtaacata
     4201 ttgcagattt tgttgtgata cgttagaaag aatgggtgca aaagttatat cgtatggatc
     4261 aacatcttca gggagattta ccataataat tccatgctct ataagccact caagtccaga
     4321 tatgaattca gagtcaggaa tctttccttc tgcccaccaa cctgcattgt tttttatcca
     4381 ttttggtatt tcatatccag ctactgcttc ctgttcggta gtagatacag ttatgatacc
     4441 attcgttatg agccactcga ttcccgcaac aaatgaagca tcaaagatct ttccttctgc
     4501 ccaccaacct gcattgtttt ttatccattc tggtataggt tcaaatgatg catctccata
     4561 tttatcaaaa gtgtattcag tttgcatgcc aaaagttgat tgagtgccaa ttcccatcaa
     4621 gagaaatgaa gttatggtga ttattaataa agaaaatagt tttttcaata tattttgcaa
     4681 caattaatta cattaaatga tatctatgct gttctcattc tatcattata gattatacaa
     4741 attcaaaatt tttgtgcgta gtataataac gaaatcttac ttttaataaa cgcaaaacca
     4801 aggtgttgaa gtatcaaatc tggtctgctt gtttcttttt tttagccttt gatatggtcg
     4861 tgttaaatga cgatgtgccg acataactgg caaataaagc tgacattgta tttttcgtgg
     4921 aatttctgaa gtgatttttg aggatgattt acttccacat ttggtacatt caaatccttg
     4981 cttacttcct ttcgatttca tttttttact gcatttttta caggatggat tactggctcg
     5041 gatatctttt gctaattcca tcacatctaa aaattcaaca ttaactattc tttcgtgatt
     5101 ttttgatgct tttctaattc ctccaccaat tcgaatttta tcatttaaaa taagattttg
     5161 tgctatcgtt gtaatttttg ttggtttata gattccacat ttgatcttct tgtccttaac
     5221 tctgattgaa aaaaatacat gaccgccttg tgccatgatt gggctactac atacttgacc
     5281 agccaaaaat ccagatgtat aaggtctcaa atcatttaca tctaattcat tttttagatg
     5341 atctgaagtt ccctgatttg atttgaatac catatatcca tctaattttt catttgcttt
     5401 tactatttta gatgctgtca ccacagattc agcgttttct ccccgtatac cataaaatac
     5461 aggatcaggt ccgtgtggag tgattaaagc tctatcagat tcaatatcat agctgttaaa
     5521 ggtttcagga aaagttatcg attgcatatt ctttacacta tctttagcaa tgattctttt
     5581 tttgccaaat tgtgattttt ttctatacga taataattca aatgtgtgat cagaaaattt
     5641 gtatccaatt gcgcctattg ctccaaccaa accctgaccg tttcctagat aaaatgaatc
     5701 aattttattt tctaaaataa attgtttagc tttttctcta ctgattaatt tccaaagtgc
     5761 aagttcacta aatttatgaa atgagtgtgt aactatctca ttttcataaa aaacaagtcc
     5821 tggatttgca ccattttttg tttctgaata atcatccaca aactttgtaa ttttgttttt
     5881 tattttttta ggattgttag ttttgatact taatctgaca gctccattac ctcgtgtttt
     5941 ccatggaatg ttggggttaa atctaattaa agatggataa tctacaaatt gtactttatt
     6001 cttttcaaga aatttaacaa ttttgtatgc aagaaaagta gtacacatac cttttggtga
     6061 gtctgtgtcg tctattccta tatgtattac agtactattt ttcaatactt tttatcctat
     6121 attcttacat ttttgggttt tgaatgcaag cttgatttaa attaaaaaca tagtatctgt
     6181 cataatacaa atgataataa ttgatgagag aaaaattttt gatgtcatag aagcaaagaa
     6241 accagtttct gtcgccctaa atggacctga tggaatactc ccaaaagtgc aagatcttgc
     6301 gttaaaaatc aatcaaaaat gtggaattcc ggcgtatctt cttgcagatg ccacttgggg
     6361 ctcgtgtgac ttgaattcaa ttggagcaaa cgtcttacaa gctgaaatat tattcaacat
     6421 aggccatacc aataaactag aaatatttga aaaaaatatt tacatgattg atgcttatga
     6481 cgatatacca tttgataatg ttgtagaaaa atgtgttaaa cagtttaaag gaaacaccgt
     6541 ttcgttgtta acagacagtc agtatcttca tcagattgac catattgcta aaacattaga
     6601 aaataatgga gtaattgtaa aaattggaaa tgggaaagga caacttaatg atggacaagt
     6661 ttttggttgt gaattctatc ctgcgacaga aattaaaaat gatgttgacg ccaatatttt
     6721 tttaggtcag agtaatttcc atgcagctgg tattgctttg gctacaaaca aaccaactta
     6781 cattttagat ccatacttca acgaagtaag agaagtaacg gaatttgcta aaaaaatgca
     6841 aaagagagct accttagaaa tttacaaagc agctgatgct gaaacttttg gtgttatagt
     6901 tgggctgaaa gagggtcaac tttctaaact gactgccctg aaattcaaaa aagaattaga
     6961 agactgcggt aaaactgttc atcttatagc acttacagat atcacaaatg aaaggttaca
     7021 aaacctaaag gatattgatg catttattca agtggcatgt cctagaattt caactgataa
     7081 caagttcgat aaaccagtct tatcaactcc ccaagctcta tctctcctaa aggtactcag
     7141 aaaagagggg attggtgagt actttgagag aaatcattgg ctgtaaacaa ttaataatta
     7201 taatttaaaa aactaaacat gtcagacacg gtattaccag gagataaaat tgctttaatt
     7261 gaagaatgtg aagcgggtaa aaatagctat gatgatgatg ggttgataag atctatgata
     7321 gttggtaaaa gtgaaatgaa taaaaaagaa cgtttgattg atgtagaaaa tcaaaaagta
     7381 ctgtcagtac caaagacaga tgatattatt attggtacaa ttgaaatggt aacaccatca
     7441 atgattgcag tgttaattaa ttacataaat gataaaccag taaaatcaca agttgagtgt
     7501 atttgctcaa ttagaaatct tcgtaaaaga agtcctgcac taataaaaga tatagtgaaa
     7561 gcgagagtga taggaactct aaatggagtt atacatgcaa caatacaaga taaagaacta
     7621 ggagtattat ttacaaaatg tagaaagtgt ggtcaagatg ttgtactatt tcgtgatatt
     7681 gtgaaatgta ttgaatgtgg ttggacagat gatagaaaaa tatctactga ttttttacaa
     7741 agcaattttt taaaaatgag agagtgaaat atgacacgtg tttcgttcca gggtgaacgg
     7801 ggagcatata gtgaagccgc atctatttcc ttctttgata atacaataga tacaattcca
     7861 tgtactactt ttgctgatgc attaaaaaac acagaaaatg acatgagtga ttactctgtt
     7921 ttgcctgttg aaaattcact tgaaggaagt gtaggtgaaa gtaatgactt gttggtaaca
     7981 gtgaatcttg acgttgttgg agaaatatat catcgaatac gacattgtct tataggaact
     8041 ggttcacttg aaaatattga cacagtttat tcccatacac aagcattagg tcaatgtaga
     8101 aaattcattc aagaaaattc attaaaaact gttccatctt atgataccgc cgggagtgtt
     8161 aagatcatca aagatttgaa taaaaataat gtagcatgta ttgcaagtaa gaatgctgca
     8221 gaaatttttg gtattcctgt aattaaacag ggaattgaag ataatagaaa taattataca
     8281 cgatttcttg tattatctaa agaatccaat gaagattcac ttatgtccaa acagaatgat
     8341 tcgtcaaaga agaaaacttc aattattttt tctgtaaaac atgaagccgg tgctctatat
     8401 caaatcatca aggaatttgc tacgtacaaa ataaacctaa caaagataga atccagacca
     8461 aacaaaaata cagcttggga gtacaacttc tatgttgatt ttgaaggtag tcaggacgat
     8521 tctatgatta aagaaatgtt gcaaaaaata aaaggaaatt cctcttttct taaaatttta
     8581 ggtacgtatc ctatgacaaa ccttgattag aatcctcgtg gttttcttgc gctaaatgct
     8641 gcactgaatc cgataacaac aatacccgct atgatcacca gtatatgata tgtgaaaaat
     8701 aatggatcac gggataactc aaatgatcca gatatattga attcagattt gccagtattt
     8761 tgaatttgaa tcaagttttc tccagaatct gtatttgtcc atgaaaaagt tgcttttcct
     8821 ttatagctat tatcaacatt atttttttca tttggagtaa agatcttcac atcaaatgta
     8881 tctccagtta tttctaacat ttgaaccgag tcagcaggtg cattaaatct aattctatca
     8941 gaatcaccta ctccaaaagt atcattggtt tctctaattt cattgggata tgccaatgaa
     9001 tcaactagag aagctatgcc gagtccacaa attacacaac cgacaattaa accgataatt
     9061 gtgtatttgc ttaacataaa aaaaattcca aaaggctgaa taaaaaacta actacatgac
     9121 atcaacatca tgaggctggc tttcagcaaa tccagctcta gatattttta taaattctgc
     9181 atttttttgc atttgttgaa tgttatgtgc accacaataa cttaaccctg aacgaattcc
     9241 tcctgtcatt tgagtaataa tatcggttac acttcctcta tacggaacca taccttctac
     9301 accttcagct acataatcat tcaagtcatc tgttagtcca gcgttaccac tatcttttga
     9361 ttttcttccc agtgaagcgc ccaaggaagc cattccacga taaatcttaa agcgtttgcc
     9421 attttttgaa attgtcgagc ctgggctttc atcagttcca cctaaaatac ctcctatcat
     9481 gactgaatgt gctccagccg ctaatgcttt tgtggcgtct ccagaagttc ttgttccacc
     9541 atcagagata attggtatgt tatgttcatt accaattttt gcacaatcca aaactgctgt
     9601 taattgtgga acacctgaac cagtgataac cctagttata caaatagaac cagagccaac
     9661 gccaactttt actgcatcaa caccagcttt aatcaaatct tccgtaccgt gtgctgtggc
     9721 aacatttcca gcaattaatt cacaattggg aaatgctttt ttgatatttt ttactgtgtt
     9781 tatggcattt tcactatgac cgtgcgctat atctacaaca ataacatcga caccagcatc
     9841 aagtaatgct tcagtccttt gcataaaatc acccttaact ccaactgccg caccaacaag
     9901 tggacgacct ttttcatctt ttgatgcatt aggatatttt tctaaatttg aaatatcctg
     9961 gctagttatg agacctttga tttggttatt ttcgtcagtt aagggtaatt tttcaattct
    10021 attatttttt aaaatttctt tagcttgttg aaaatcaatg ccgggttttg cactaacaac
    10081 atcttttgtc atcagatctt taactaattt agaacaatca actggctcaa ataagacatc
    10141 acgctcagta agaataccta ttagcttaga gctagaatca gtaactaaaa gtcctgatac
    10201 ctgcttttca ttcataattt taaaagcatc ttgaattgtt tgttcaggat taatcagata
    10261 tggattttct atgattacac ttccagcacg tttaacttta tgaatctcat caacttcttc
    10321 ttgtatagtt aaaaatctat gaattattcc tataccacct tcacgtgcaa ttgttaccgc
    10381 cattgttgat tcagttactg tatccatgtt agcactgatc attggaatgt taatcgagat
    10441 gtttctagaa agttgtgtag ttaaatcggt ttgtgaacga ctaactatat ctgaaaattt
    10501 gggtacaaga aggacatcgt cgaatgttaa tccttctctt atgctcatga aacctcatac
    10561 cttagaaaaa aaaaccgtta taagcctttc ttgtattcat gataatcttt gagcaatgat
    10621 tactgcatct tttgtttcac ttacattatg cgtgcgtatt atgtgtgctc catttagaac
    10681 agatataacc tctgcagcca gtgaaccata aagtctatct gatggatttt ctttttccag
    10741 tattttacca agaaacgatt tgtttgatac agaaacgaga attggcttat tcaatcttat
    10801 cgctcttaaa ttttgcaaaa ttgttaagtc acgttgaatc gaatcagaat ttattttagt
    10861 aaatattgca gattttttac ccttatttct aaaaaatcct atacttggat ctaaaacaat
    10921 ttgtgactgg gaaatatttg cagatttaga tatattcaga ctttcttgta acaaattttt
    10981 tgttttctgt acttggttac cagttatggt ttttttacta tatgcacata agatcaatga
    11041 aggtgaatat ttctcaacaa tttttggcat taatggatca tattttaatc ctgtaatgtc
    11101 attgattata tcaacaccta attccaaagc ttcttttgct acagatgatc tacatgtatc
    11161 tacagaaata ggcaagttag aatgctgctg aacaatctta attcctttga tgatacgatt
    11221 tatctccatt ttttctgtta tcattgttga tagatatggt gctgttgaca taccaccgat
    11281 atcaataaaa tctgctcctt cctcctgcat tctgttaact gtatcaataa ttttttgttt
    11341 ggaacttgag atagatttct tatagaatga ttcaggactc gtgtttaaaa tccccattat
    11401 tcttacagga ttagagctac ccactgaaat agttcctaat ttattcacac aatttatcat
    11461 gatccttgct aatttgagtc atatgacaat tgtaggttgg gaatcaagat atagagaaat
    11521 tctgaaagaa tttggttatt caagaaataa tgataatcaa tcatgtagac tattagattc
    11581 gatactacca aaaaaagtag atcttgtaaa aattagatgc ttgatagaga ataaacctgt
    11641 tttcatagtt ggtgccggtc catcattgcc atcttccatc ccaattttga agaaatataa
    11701 aaaaattaca aaaattgtag ctgatggcgc tacgcaagca ataattgaaa atggtttgaa
    11761 acctgatatt gtggttactg atcttgatgg tgatagcaaa tcgctgaaaa aagctggtag
    11821 aacaaataca atgatggttg tacatgcaca tggtgataat tctgaaaagc ttggttttgc
    11881 aaaaaacttc aaaaattgta taggtacaac gcaagcaaaa cctacaacaa acatacataa
    11941 ttttggaggg tttacagatg gagatagatg tgtatttttg gctaatcatt ttaaagcaaa
    12001 gaaaataatt cttcttggta tggattttgg aactaagatt ggaaaatact ccaagagaac
    12061 aatatctaat cgaaggatca aaattgcaaa attacgccgt ggaaagaaat tactcgaatg
    12121 gcttgcacca aaaagcaaat cagatctcta ttctacaact aaaatcaatg gattcacaaa
    12181 agtaaatcta cgaagcattg ataatataat agcaaaaaat tagatatact aaatgaaaat
    12241 tatacaactc aattctgcat caaatctgat tgaggattca atcgacggtt ttcgtgtgaa
    12301 gatattatgc gacccatggt tgaatggaga agaatatcta ggctcatggg caatgtatcc
    12361 tccatacaat tttaaaccag aaaatttttc agatttagac ttcatataca ttagtcatat
    12421 tcatcctgat catagttctg ctagtacact atctaaatta gataaaaaaa ttccagtgtt
    12481 aattcataat tttccagaaa aatttcttaa aaataaaatt gaaaatcttg gttttaaaac
    12541 aattcaacta gaacatggag ttagaactag attaaagaat aatctgcatg tgaatattat
    12601 ggctgcagat aactgtgatc caaatgtatg tgggaaaatt atgggttgtg gtttatctga
    12661 ggttagattt caaacaactc agatagatac aatggcagta tttgataatg gaaatcaagt
    12721 aattgtcaat actaatgact gtccatttga tattgcaaaa aatacagcgt ctataatcaa
    12781 atcaatatat cataaaattg atgtactgct tcttggatat gttgctgcat cctcatggcc
    12841 acatagctat attcttcctg aagtggaaaa agaagaggca gccaagaaaa aacaagatgt
    12901 taaattacaa actgcaaaaa aatacataga attactgcaa ccaaaatatt acattccttt
    12961 tgctggtcgt tacacactat gtggtaaaaa tacagtttta aacccatata gaggagaacc
    13021 agagcttgag gatgcgtttg aatggttaca taataatata gaacaggaaa atcaccgcgg
    13081 aataattttg aatgatgatt gttggtttga tgtagatact ggttgtacta gcaagaatta
    13141 tgttccaatt aacaaaaatg aaaaggaaga ttatgtcaat aatatactat caagaaaaaa
    13201 atttccttat gaaactcaac ccatacccac tactgcagaa atattagact tgattccaaa
    13261 agcctatgaa aattttgata aaatcaggca aaaaataggc tggatttctg atactacagt
    13321 aattctaaaa accactaacg atgatttggt cattgcaata agctgtcagg gaaaaggatt
    13381 taagaaaata tctaatgatg atgtaaaaaa atatgagcag tgtatgataa tatctttaga
    13441 cgctcgatta ctgaaatggt tactacatgg accatataaa gcacattgga gtgacgcaga
    13501 tcttggttct catttaagat atgaacgaat tggaagtatc tacaaacgtg gtttatttta
    13561 ttgttggaat catttcagcg ctaacgtata gtaagaatgc gttttaatat tgttctagat
    13621 aagaagatgt atgggtcttt ctgatgagca aattcgtgat atgttagaat taaaagaaga
    13681 tcttgaggaa aaaataatca aatataagga acaaattgaa aaactggaaa aaaatatttc
    13741 tgtgttagat tcaatactta aacaatcaag ttttgcaaaa gcttccgatc ttactgtaga
    13801 tacaaaaaaa caggcagtat caattccaat tacaaaaaat tctgatggaa caactattgc
    13861 aaatgtattt gtttcagacg ataaagtttc aattgtttta gaagataacg taacaattaa
    13921 tcctgaaaca cctcctctga aaactttttt tgttgataga ataattggtg agatgaaaaa
    13981 aaaggataat caagaagctg aaaatggtac aataaacaaa gatgatatta ttgattgtat
    14041 gataaaaaat aatggaagtg cattaagaga aattataatc aaaaattacc gtcaaaaaga
    14101 aagagttgat gagattataa ccactaccac atggtctctt agaaaaatgc tagaaaactc
    14161 agagtgattt aatggacaaa attgttgttc tagattttgg ttcacagtac agccatctga
    14221 tatgtaggcg tattcgtgat ttttcagttt acgctgaact tgttccatac aatatatctc
    14281 tagaagaatt gaaaaagcta aatccaaagg gaattatttt ttctggtggg ccatcaagtg
    14341 tatatgattc aaatgcacct atgcccaaca aggaaatttt ccagttacaa ttaccaatat
    14401 taggaatatg ttatggtcat cagataattg tcaataattt tggtggccaa gtaaagcgag
    14461 caaaaaaaga atatggctca tcaattcttt cagttgataa taattcagat attctttcag
    14521 gtattggtga ctctattaga gcgtggatga gtcatggtga tgaagcagag aatgtaccaa
    14581 aaggttttga agtaattggt catacagaaa actcacaaag tgctgtaata acaaataatg
    14641 aaagaactgt ctatggtata caatttcatc ctgaagtcgt tcatacagaa aaagggattg
    14701 aaattctaaa aaacttcgtt ttgaaaattt gtgatgccaa gcaagactgg agtatggaac
    14761 agtttgtgga aaattcaatc gaaaatatta gtaaattaga tggaaatgtt ttgtgtggag
    14821 ttagtggagg aattgattct actgttactg cattattaat ccataaagca attggagata
    14881 aattaaaatg catttttgtt gataatggtc ttttgagact gaacgaagta gaggaaatag
    14941 aaaatatgtt tgtaaataat tttaaaatca attttgtaaa gatagatgca ggaaaacaat
    15001 ttttatcaga attaaatggt gttgtggatc cagaggcaaa aagaaagatt gttggagaag
    15061 aatttgttaa aatttttacc aagtttgcaa aagataatgg tccgtttaca tggcttgcac
    15121 aaggaacact ttatcctgat gtaattgaaa gtggagtgtc aaaaggacct gcatctgtga
    15181 tcaaaacaca tcataacgta ggaggattac cagattggtt agaacttaag gttttagaac
    15241 cattacgtga tttgtataaa gacgaagtaa gaaaggtagc aaagattctt gatgtaccag
    15301 aaaatttga
//
LOCUS       DASCPJ010000114        13050 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001790749, whole genome shotgun sequence.
ACCESSION   DASCPJ010000114 DASCPJ010000000
VERSION     DASCPJ010000114.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13050)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13050
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001790749"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="QGF01_05535"
     CDS             <1..1076
                     /locus_tag="QGF01_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HJN20380.1"
                     /translation="PEIKKVLAYSTGSQIGYMMMALGVAGLSQQYVDGYTAGFFHMIS
                     HAMFKASLFMAAGSLLHIVGSRFMTDMGGLRKKMRKTYAFMWAAGLALMGAPFITTGF
                     WSKDAIFAAVYESGNEWALPIFIIAVLTAVITAFYTTRMLGMVFFGKESKHIEKMESK
                     GHHIHEAPRSMWIPYGILAVLTIGIGIVGFSAEEGIHHLFTEYLDESFGIHAPHIDVE
                     HSESLGFLSGLNPVAVGASLIAFSVGFGLGYIFYIGRWVDPAKFVNSNIFFYSLHKLM
                     LNRWYLNAMIYWGFVTAPLWASRALWRYFEKTAIDTGMNVGLERAVGWGAKVVQGTQT
                     GVAQSYLYVFGAGLLFVVLILLV"
     gene            1087..2571
                     /locus_tag="QGF01_05540"
     CDS             1087..2571
                     /locus_tag="QGF01_05540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HJN20381.1"
                     /translation="MIELTSTPIIILAILGSVGVALPLMGIAMKEKGTVVSYGIVTLL
                     ALFGSIGYILYQLALDHVSPAAIFSEDVLVDDAFGAFFAIAMLIVAIFTTVGSFSYMK
                     NKSNIPVYFSLILLSTIGMVLVAYATDLVMLFIAWELMSIPTYILVGFLKKDPISNEA
                     AIKYFLFGALASAIIIYGISLAYGITGSTNIGEVIQGFMVLEANLIPVALLAVGMFIA
                     GFGFKMGLVPFHMWLPDAYEGAPPTITTLLAAGTKKAGFAAALRVIVMGTVALSLDWT
                     LALGIIAVMTMTVGNIAAVMQRSLTRMLAYSSIGHAGYILIGLSIAPFSPIGIQGSLF
                     HILNHAVMKGVAFIAVAGIVTALAVTHIEKIKGLGRRMPITSLGLVISLLALAGVPPL
                     SGFWSKLMLFGGAIDAGTVVWWGPWIAVAGVLNSALSLAYYGWIIRKMYFEGETEKRI
                     KEPRSIIAVMIFSIIFMVTIGVYPEPIIQFAEYATPTLGIISTP"
     gene            complement(2561..3418)
                     /locus_tag="QGF01_05545"
     CDS             complement(2561..3418)
                     /locus_tag="QGF01_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HJN20382.1"
                     /translation="MKVLYRKNLEANLIANYSEYVKKINSALDTELDLYSKSEFKEPL
                     KYALGEGKRIRPIILLLSSECVGKINDSALALACAIEFLHTESIIHDDIIDNETVRRR
                     KDPFYIKYGYNTSVLTGDFVLGLILNIASRINHQKITKNLATAAMLMSEGEIIETRLE
                     TSEDVTFNDYLKVIEYKTAIAFETACRLGAIIAGGTDKEIEYLASYGKNVGIAYQIRD
                     DLHDWKNEDKLFNFIIKKSTDPRDVFNHMDELQKEYSELALSSIRKLNDCNAKSSLET
                     LVMLTKTKV"
     gene            complement(3454..3528)
                     /locus_tag="QGF01_05550"
     tRNA            complement(3454..3528)
                     /locus_tag="QGF01_05550"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3490..3492),aa:Glu,seq:ttc)
     gene            3610..4245
                     /locus_tag="QGF01_05555"
     CDS             3610..4245
                     /locus_tag="QGF01_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HJN20383.1"
                     /translation="MSNLIQKIDVLIEERSLLKHPFYQTWSEGKLTQDALAGYSKEYY
                     QLVKAVPRFMTPLLDTAPESMYDELDFNQNEETNHIKLWIKFANALGVSAYDLESYNG
                     LEKTTMAIEGLFSLMYSFDTGSSAMYALEKEIPKISKTKLEGLAEYYGLTSFDATVYL
                     KQHTEADIRHAQSWENAIDASSKDEQTILDAANNSLACQNLLLDGCFETYC"
     gene            4287..4360
                     /locus_tag="QGF01_05560"
     tRNA            4287..4360
                     /locus_tag="QGF01_05560"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4321..4323,aa:Ile,seq:cat)
     gene            4575..5138
                     /locus_tag="QGF01_05565"
     CDS             4575..5138
                     /locus_tag="QGF01_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HJN20384.1"
                     /translation="MTETKPIVSIENVVASASVDQAIDLADVTKKFPSTEWHPDQFPG
                     LVFRLTSPKTATLIFRSGKMVCTGGKSEDMAIKAVRSVVQQLRKGKIKIKKEAVITIQ
                     NIVASGSLGGKIHLEQAARKLPRSMYEPEQFPGLIHRMVDPKTVLLLFASGKLVCTGA
                     KKESDVYKSVHNIHAVLEDKDLMLYDA"
     gene            complement(5126..5737)
                     /gene="pyrE"
                     /locus_tag="QGF01_05570"
     CDS             complement(5126..5737)
                     /gene="pyrE"
                     /locus_tag="QGF01_05570"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HJN20385.1"
                     /translation="MEFVKEFAIFLYKNNIIKFGNFTLASGKNSPYYIDLRLVPSYPH
                     QFRKMIKNLQNLIDKKTGLKNFDALVSVPTGGLVIASALAIETVKPLIYVRNKSKEYG
                     TSKLIEGKLSSNMKLVMIDDVATTGNSILNGIKQLQEAGSIISESYVIVNRLENANKA
                     MDSVGVKLYQLTDILEITKILFEEHLIDEEILEKIKKQIDQAS"
     gene            5789..6862
                     /locus_tag="QGF01_05575"
     CDS             5789..6862
                     /locus_tag="QGF01_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJN20386.1"
                     /translation="MPEIWLNYGANEVVLDIKAENLEQKTEIKGTNLSDSEIDSKLEL
                     LDISKPTEIVVLNASDAVRKTLSVLFEKYEKQSIQKPVILADKEILPMLKKYLPEHNV
                     SEFNNLENSNLVFLGEMEFDGLFGFETISTRLLKKFGTELMLSAYEKRKNDQPSPGEN
                     VESFKIAQKFSKKFDILGIEIIANSNGICDLSVDHPSSTSSLTKAFGSYTAADIGKHK
                     TMIISTGKESSNHTLGKSLSSLWNCTNALKNDGYAILTAECRHGIGSDAIRHYIDDRL
                     NIDQLKKPSKYIDGMENLLYLTEIQKKFQIGILSILPEFYTKKLNMLSFNGIKKVMDY
                     ILKTQGQNQKIKVISDGARTFLR"
     gene            complement(6854..7933)
                     /locus_tag="QGF01_05580"
     CDS             complement(6854..7933)
                     /locus_tag="QGF01_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJN20387.1"
                     /translation="MTEPSQDDVTQIVSSIFDIQETVVFAETLRFKIDSYDFKDKFVR
                     LAQNLELLGMVTRLEKSYDGLYLDISRIAKPKKKWLSKSFVPRILFVVTVIMVLIDGY
                     YRTDFVNSLSFIGNPTEMSILYAFSLIGILGVHESGHLIAAKKHKIKTTWPYFIPGVP
                     VFGIPTFGALIQSRGLTINRDILFDVAIAGPIAGLIIAIIVSMFGALTSPVIDNMLAD
                     ELNDGWQLMEMNMPIFMIISLEIFDKGDGHVIMSPIFFAAWLGFLITFLNLLPAWQLD
                     GGHMARTLLGKKWHKIATYASTGLLAALGYWFMALFILFLSSRSRDMKPLDDISPLSK
                     NRKKLFVVIVILGFLCAPLPSWILP"
     gene            8010..8510
                     /gene="rimI"
                     /locus_tag="QGF01_05585"
     CDS             8010..8510
                     /gene="rimI"
                     /locus_tag="QGF01_05585"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HJN20388.1"
                     /translation="MQVILRQIGNCIIRRCDPTDIIPVMEINLKTLPEHYSDYFYESL
                     LDELPEAFLIAEISGKLIGYIMCKTEYGFSNFKKLGFVKKGHVVSVAVIDEHRGKGFG
                     SELVNESLKGVKMRQCGEMYLEVRCSNNDAVKLYEKLGFSINQRLKTYYRDGEDAYLM
                     AINFSD"
     gene            8545..8769
                     /locus_tag="QGF01_05590"
     CDS             8545..8769
                     /locus_tag="QGF01_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJN20389.1"
                     /translation="MSRRKFGGIIIFILAISAFFVYAYLIMLSEWSPIVLQLSILTII
                     GGILGVISWIGYVMATTKSSPSSTIQDDET"
     gene            complement(8771..9598)
                     /locus_tag="QGF01_05595"
     CDS             complement(8771..9598)
                     /locus_tag="QGF01_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HJN20390.1"
                     /translation="MLKKALENILSEKESMELFSSFDQVGEIIVLRIPDSLQAKKKII
                     GETLLEQVKTARSVFYQSSPVEGEFRTRSLEILAGIDNTQTEYKETGCKFIVDVEKAF
                     FSPRLSTERERIAGLVKDGEVVVNMFGGIGMFSILIAKTKKCTVYNIDINPEAAKLCN
                     KNIEINKLVGNVISIEGDSSEIKKHNIENKSDRTLMLLPEQSDKFLDSAILTTKNNGI
                     IHYYSHIHADKKSDAPKLSEQHFLETTSVKSTILGSKIVRAVGPRFYQTVVDVKIKK"
     gene            complement(9604..11388)
                     /locus_tag="QGF01_05600"
     CDS             complement(9604..11388)
                     /locus_tag="QGF01_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HJN20391.1"
                     /translation="MTHRIAALDKDLCQPKKCGLECIKYCPVNKSGADCIILNEETKR
                     AQIDEDICNGCGICVKVCPFDAITIVNLATELSTDKIHQYGINSFRLYKLPTPKKGEV
                     VGLLGRNGMGKSTIVNILSGNLKPNLGNYNEPPDWDEILRYYSGTELKSHFEQISENI
                     IKSSIKPQQVYDIVNAFDGTGKELLAKYDERGITNDLVKKLDLKNSLEQNLQELSGGE
                     LQRLAVAITASKNADFYFFDEPSSYNDVFQRTGVARVIHSLAELGKSVMVVEHDLTLL
                     DYLSDYIEVLYGVPAVYGIVSGIMSTKVGINVFLDGFLPNENVRFRDKKFTFDASTSQ
                     DEFQHGDKIISYPKLVKKYPTFSVTIEPGFVKKGQLLGVMGANALGKTTMMKMIAGVE
                     KPDIGTVEKKLKIAYKPQYLSNDIDVEVVSVLDKANQSPIEDSQEEEQIVDPLKIKKL
                     YNKSIKNLSGGELQKVAVASCLLQKVDVYALDEPSAFLDVEDRIAIGKFLQKFVRSFG
                     KSAIIIDHDLQLMDLVSDSMVIFEGTSSIDGHGSSPLPKSEAMNRFLKSLEITFRRDE
                     KSLRPRVNKEGSRLDKQQKNSINYYYRN"
     gene            11505..11624
                     /gene="rrf"
                     /locus_tag="QGF01_05605"
     rRNA            11505..11624
                     /gene="rrf"
                     /locus_tag="QGF01_05605"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(11803..12198)
                     /locus_tag="QGF01_05610"
     CDS             complement(11803..12198)
                     /locus_tag="QGF01_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20392.1"
                     /translation="MNIDLKIQDSVPLMIYDDECYVCIQFAKLVNYLAKGRLYLVGHY
                     TDFGKDLRDNFLDSNALKMFWFIDSKTAYGGRAAISPLFSAILNSSESFPKKKIQKKN
                     TLRCKSPAAVFFRSASLLTNSKKIKIPNN"
     gene            12291..>13050
                     /locus_tag="QGF01_05615"
     CDS             12291..>13050
                     /locus_tag="QGF01_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HJN20393.1"
                     /translation="MGLNYYQIKKQVLKARKLVIKATAAAGSGHPGGSFSMAEILGCL
                     FYKYLKYDPKNPGWEDRDKLILSKGHASPGLFSNMAVAGYFDPKDIVTLRQFGSKLQG
                     HPDLKCPGVEFCGGSLGIGLSYSIGNALAARLDGKNNRIYTIIGDGESDEGQVWEAAM
                     TAAKYKIDNITAFLDRNFIQQDSYTEKIMPLDEELIGNDLSEMWKDASRWKTGDKWRS
                     FGWNVIEVDGHRTEQISDAIRRANETKGLPSIIIA"
BASE COUNT         4263 a         2162 c         2193 g         4432 t
ORIGIN      
        1 atcctgaaat caagaaagtt ttggcatatt caactggttc acaaattggt tacatgatga
       61 tggctcttgg tgttgctggt ttatcacaac aatatgttga tggatatact gctggattct
      121 ttcatatgat atcccacgcc atgttcaagg cttcattatt catggctgct ggttcattgt
      181 tgcatattgt tggttcacgg tttatgacag atatgggagg tttaagaaaa aagatgcgga
      241 aaacatatgc attcatgtgg gctgcaggtt tagcgctgat gggtgcccca tttattacca
      301 ctggattctg gagcaaagat gcaatttttg ccgctgttta cgaatcagga aatgagtggg
      361 cattaccaat tttcatcata gcagttttga ccgcggttat tacagcattt tacaccacaa
      421 gaatgttggg tatggtcttc tttggcaaag aaagtaagca tatcgaaaag atggaaagta
      481 aaggacatca tattcatgaa gccccacgtt ccatgtggat tccatatgga attctagcag
      541 tattgacaat tggaattgga atagttggat tttcagctga agaaggaatt caccatctct
      601 tcactgaata tcttgatgaa tcgtttggca ttcatgctcc acacatagat gtagaacatt
      661 cagaatcatt aggcttcttg tctggtctta atccagttgc tgttggtgca tcattgatag
      721 cgttttcagt tggctttggt cttggatata tcttctatat cggtcgttgg gttgaccctg
      781 caaagtttgt taactcaaat attttctttt actctttaca caaattgatg ttaaatagat
      841 ggtacctaaa tgcaatgatc tattggggat ttgttactgc acctttatgg gcttctagag
      901 cactatggag atactttgaa aaaactgcaa ttgacactgg catgaatgtt ggattggaac
      961 gcgcggttgg ttggggtgca aaagtagttc aaggaacaca aactggtgta gcacaatcat
     1021 atctttacgt atttggagca ggattactgt ttgtagttct gatattatta gtataggtga
     1081 tatgagatga tcgagctaac atcaactcca ataattattc ttgccattct aggtagtgtt
     1141 ggagttgcac ttccactaat gggtatcgca atgaaggaaa agggtacagt agtatcatat
     1201 ggtatagtaa ctctccttgc cttatttggt tcaattggat atatcctgta tcagttagca
     1261 cttgatcatg tatcacctgc cgcaattttt tcagaagacg ttcttgttga cgatgcattt
     1321 ggtgcattct ttgcaattgc aatgttaatt gtagcaatat tcacaacagt cggctcattc
     1381 agttacatga aaaacaaaag caacatacct gtttattttt cattaattct tttatccacc
     1441 ataggaatgg ttcttgtagc atatgctaca gatctagtaa tgctctttat tgcatgggag
     1501 ttgatgagta ttccaacgta catactagtt ggatttttga aaaaggatcc aatttctaat
     1561 gaggctgcga taaaatactt tttgtttggc gcacttgcat ctgcaatcat aatttatggt
     1621 atatctctag catatggaat tacgggttct acaaacatag gtgaagtaat tcaaggcttt
     1681 atggttcttg aagcaaattt gattcccgtc gcattattgg ctgtaggcat gtttattgct
     1741 ggatttggct tcaaaatggg tcttgtacca tttcatatgt ggttaccaga tgcatatgag
     1801 ggagcaccac caactattac aactctatta gcagctggta caaaaaaggc aggattcgct
     1861 gccgcattaa gagttattgt catgggaact gtggccctaa gccttgattg gacacttgca
     1921 ttgggtatta ttgctgtcat gaccatgaca gttggtaata ttgctgctgt aatgcaaaga
     1981 agcctaactc gaatgctagc ttattccagt attggtcatg caggatacat attgattggt
     2041 ttgtctattg caccattttc tcctatagga atacaaggat ccctatttca catactgaac
     2101 catgctgtga tgaaaggtgt agcattcatc gcagttgctg gaattgtaac agctctagcg
     2161 gttacgcata ttgaaaaaat aaaaggtctt ggaagacgaa tgcctataac atcattagga
     2221 ttagtaatat ctttacttgc acttgcaggt gttccaccgc ttagtggatt ttggagtaaa
     2281 ttgatgttgt ttggtggtgc aattgatgct ggaacagtag tatggtgggg tccatggatt
     2341 gctgtagcag gagttctaaa cagtgcatta tctttggctt attatggttg gataatacga
     2401 aagatgtact ttgaaggtga aacagaaaag agaatcaaag aaccaagatc aattatagct
     2461 gtaatgatat tttcaataat attcatggtg actataggag tctatccaga accaataata
     2521 caatttgcag agtatgcaac tccaacactt ggtattatat ctacacctta gtcttcgtta
     2581 acataaccaa agtttctaaa ctacttttgg cgttacaatc attcaatttt cttatactag
     2641 aaagtgctaa ttcagaatat tctttctgca attcatccat atgattaaaa acatctctag
     2701 ggtctgtact ttttttaatt ataaaattaa ataatttatc ttcatttttc cagtcatgta
     2761 aatcatctct gatttgatat gcaattccaa cattcttacc gtagcttgct aaatattcta
     2821 tttctttatc tgtaccacct gcaataatag cacctagtct acaagcagtt tcaaatgcta
     2881 ttgcagtttt gtattctatc actttgagat aatcattaaa agttacatct tcacttgttt
     2941 cgagcctagt ttcaataatc tcaccctcac tcattaacat agcagcagta gcaagatttt
     3001 tcgtaatttt ttgatgatta atccttgatg caatattcaa aattaatcct aaaacaaaat
     3061 caccagtaag cacacttgta ttatatccat attttatata gaaaggatct tttcgtctac
     3121 gcactgtttc attatctata atatcatcat gaattataga ttcagtatgc aaaaattcaa
     3181 tggcacatgc aagtgctaag gcactatcat taatttttcc tacacattcc gacgataaca
     3241 aaagaataat tggtctgatc ctttttcctt cgcccagtgc atatttcaaa ggttccttga
     3301 attcagattt agagtataaa tctaattcag tatctaatgc tgaattaatt tttttcacat
     3361 attcactata atttgcaatc aggtttgctt ctaaattttt tctatacaaa actttcaatt
     3421 tgaaatatca tttagaaata tattaaactt aagtgctccc gtcgggattc gaacccgagt
     3481 ctcaggcgtg aaaggcctaa atgcttgacc ggactacact acaggagcat ataaaaccga
     3541 tctgaaggca atgaataaaa ttctttggtc actcaaaaga tttttttata accaaaatgg
     3601 gtgcttatca tgagtaattt aattcaaaaa atagatgtgc tgatagagga acgaagttta
     3661 cttaaacatc cattttatca aacttggtct gaaggtaagt taacacaaga cgcactagct
     3721 ggatattcta aagaatacta tcaactggtt aaagctgttc ccagatttat gactccatta
     3781 ttagacactg ctccagaatc gatgtatgat gaattagatt ttaaccaaaa cgaagaaact
     3841 aatcatatca aactatggat taaatttgct aacgcattag gcgtttctgc ttatgatcta
     3901 gaatcctata atggattgga gaaaacaact atggctatag aaggattatt ttcattaatg
     3961 tattcttttg acaccggttc atctgctatg tatgcacttg aaaaagagat accaaaaatt
     4021 agtaaaacca aattagaagg tttggctgaa tattatggtc ttacaagttt tgatgctact
     4081 gtgtacctaa aacagcatac ggaagctgat ataagacatg ctcaatcatg ggaaaacgct
     4141 atagatgctt catccaaaga tgaacaaacg atacttgatg cggctaataa ctcactagca
     4201 tgccaaaatt tgttattgga tggctgtttt gaaacatact gctgatttgt ataacatttt
     4261 ttactaagaa aaaatgattt ttggtagggc ctgtaactca gcttggtaga gtacccgact
     4321 cataatcggg gagccacggg atcgaagccc atcaggccca taattatttt aaaaaattat
     4381 ttttgcatat attttcaact agttttaaat caagcaataa gccagttatt ttggctgcaa
     4441 tgaagaatca gagttatagc tgataaatga tgtaaaggca tttgactgag ccgccaaagc
     4501 atgaaataga gtatattatt ttgtataccg gaatttttag tgtgatatat aaagccagta
     4561 tttaacaata taacatgaca gaaacaaaac caattgttag tattgaaaat gtagttgctt
     4621 ctgcctcagt tgatcaagca atagatcttg ctgatgtaac taaaaaattt ccatccactg
     4681 aatggcatcc tgatcaattt ccaggattag tgtttagatt aacttcacct aaaactgcaa
     4741 ctttgatttt tcgttcgggc aagatggtat gtacaggtgg taaatcagag gatatggcta
     4801 ttaaagctgt tcgttctgtt gtacagcaat tacgtaaagg taaaatcaaa atcaaaaaag
     4861 aagctgtaat aacaatccaa aatatagttg catctggtag tttgggagga aaaattcatc
     4921 ttgaacaggc agcaagaaaa ttacctcgaa gtatgtatga accagaacaa tttcctggtt
     4981 taatacacag aatggtagat ccaaaaactg tattactttt atttgcatca ggcaaactag
     5041 tttgtactgg agcaaaaaaa gagtctgatg tctataaatc agtacataat atacatgcag
     5101 tattagaaga caaagatcta atgctttatg acgcttgatc tatctgtttt ttaatttttt
     5161 ctaatatttc ttcatcaatc aaatgctctt cgaacagaat ttttgtaatt tctaatatat
     5221 cagttaactg ataaagtttc acgccaactg aatccattgc tttgtttgca ttttctagcc
     5281 tatttacaat aacatatgat tctgaaatta ttgagccagc ttcttgaagt tgttttatac
     5341 catttaaaat tgaattacca gtagtagcaa catcatctat catcactaat ttcatattag
     5401 aagataactt accctcgatt aattttgaag tgccatattc ttttgattta tttcgtacat
     5461 agattagtgg tttgacagtt tcaatggcaa gggctgatgc aatgacaaga cctccagtag
     5521 gaacagaaac taaagcgtca aagtttttca aacctgtttt tttatcgatt aaattttgca
     5581 ggttttttat catttttcta aattggtgcg gatagctagg aactaatcta agatctatat
     5641 agtaaggact atttttacca cttgctagtg taaaatttcc aaatttaatg atgttatttt
     5701 tgtataaaaa tattgcaaac tctttaacaa actccatacc aaaattaact aaactggatt
     5761 tattttgctt tacgttttag cataatttat gcctgaaata tggttgaatt atggtgcaaa
     5821 tgaagttgtt ttggatataa aagctgaaaa tctagagcaa aaaaccgaga tcaagggtac
     5881 taatctttca gattctgaaa ttgattctaa actagaatta ttggatatat ccaaacccac
     5941 agaaattgta gtattaaacg catcagatgc tgttagaaag acgttatcag ttttattcga
     6001 gaaatatgaa aaacaatcca ttcaaaaacc tgtcatttta gctgataaag aaattctgcc
     6061 tatgttaaaa aaatatcttc cagaacataa tgtttctgaa tttaataatc ttgaaaattc
     6121 taatttagta tttttaggtg agatggaatt tgacggatta tttggatttg aaacgatatc
     6181 tacacggttg ctaaaaaaat ttggaaccga gttgatgctt tctgcatatg aaaagcgaaa
     6241 aaatgatcag ccttcaccag gagaaaatgt tgaaagtttc aaaatcgcac aaaaattttc
     6301 taaaaaattt gatatattag gaatagaaat tatcgcaaat tcaaatggaa tttgtgattt
     6361 atctgtagat cacccttcat ctacatcttc attgacaaaa gcatttggat catacacagc
     6421 ggcagacata ggtaaacaca aaacaatgat aataagtaca ggaaaagaat caagtaacca
     6481 tacattagga aaatctttat catctttatg gaattgtaca aatgcactga aaaatgatgg
     6541 atatgcaatt ttaacagctg aatgtaggca tggtattggt tcagatgcaa tccgacatta
     6601 tattgatgat agactaaata ttgatcaatt gaaaaaaccg tcaaaataca tagatggcat
     6661 ggagaatctt ttatatctca cagaaataca aaagaaattt cagattggta tattatctat
     6721 attaccagaa ttttatacaa aaaaactaaa tatgttatca tttaacggaa tcaaaaaagt
     6781 aatggattat attttaaaaa ctcagggaca aaaccaaaaa attaaagtga tttctgatgg
     6841 ggcacgtact tttttaaggt aatatccaag atggtaatgg agcacataaa aatccaagaa
     6901 ttactataac aacaaacagt ttttttctat tttttgataa aggagaaatg tcatcaagcg
     6961 gtttcatatc tctacttcta ctacttagga ataaaatgaa taatgccata aaccaataac
     7021 ccaacgcagc taaaagtcct gtgctcgcat atgttgcaat tttatgccat tttttaccta
     7081 acaaagttct tgccatatga ccaccatcta attgccaggc aggcaacaaa ttcaaaaatg
     7141 taataagaaa tcctagccat gcagcaaaaa agatagggga cataattaca tgcccatccc
     7201 ccttgtcaaa aatttcaaga gaaattatca tgaatattgg catattcatt tccattaatt
     7261 gccatccatc attcaattca tcagctaaca tattgtctat tactggtgat gttaatgctc
     7321 caaacattga gacaataata gctatgatta aacctgcaat aggaccagca attgcaacat
     7381 caaataagat atcccgatta attgttaatc ctctagattg gatcagcgca ccaaatgtag
     7441 gtattccaaa aactggtaca cctggaataa aatagggcca tgttgttttt attttatgtt
     7501 ttttagctgc aataagatga ccagattcat gaactcccaa aattccgata agtgaaaacg
     7561 cgtacaaaat tgacatctct gttggattac cgataaaaga aagactattg acaaaatcag
     7621 ttctatagta accatcaatc aataccatga tcacggttac aacaaaaaga attcttggta
     7681 caaacgattt tgataaccat ttttttttag gtttggctat tctagaaata tcaagatata
     7741 atccatcata agatttttct aatctagtaa ccatacctaa aagttctagg ttctgagcaa
     7801 gtctcacaaa tttatctttg aaatcataac tatctatttt gaatcttaat gtttcagcaa
     7861 atacaacagt ctcctgtata tcaaagatag aagatactat ttgtgtaaca tcatcttggg
     7921 atggttcggt catattaaca gtaaaaatag aatctattaa ttggtttttg cctttaaaat
     7981 taaatatttg ctaaaactat tagaaaatat tgcaggttat acttagacaa ataggaaatt
     8041 gcatcattag aagatgtgat ccaactgata ttatacctgt tatggagatt aatttgaaaa
     8101 ctctccccga acactattct gattattttt atgaaagtct tcttgatgaa ttaccagaag
     8161 catttcttat agctgagatt tctggtaaac ttattggata tataatgtgt aaaacagaat
     8221 atggattttc aaattttaaa aaattgggtt ttgtaaaaaa gggtcatgtg gtttctgttg
     8281 cagtaataga tgaacatcga ggaaaaggtt ttggtagcga attagtaaat gaatcattaa
     8341 aaggtgtaaa aatgagacag tgtggagaaa tgtacctaga agttcgatgt agtaataatg
     8401 atgctgtaaa gttatatgaa aaattaggct tttctataaa tcaaagatta aaaacgtatt
     8461 atcgagatgg cgaagatgcg tatcttatgg ctattaattt ctctgactag attaaaataa
     8521 ataactacat ttgattttta gcatatgtct agacgaaaat ttggtggaat cataatattt
     8581 attcttgcta tatcggcttt ttttgtatat gcatatttga ttatgttatc tgaatggagt
     8641 ccaattgttt tgcagttatc tattttgaca attattggag gcatacttgg agttatttca
     8701 tggataggat atgtaatggc aactacaaaa tcttctccat catctactat tcaggacgac
     8761 gaaacataat ctattttttt atttttacat ctacaactgt ttgatagaat ctgggaccaa
     8821 cagctctgac tatttttgat cctaatatag tagatttgac agaagttgtt tctaagaaat
     8881 gctgttcaga taattttgga gcatccgatt ttttatctgc atgtatatgt gaataataat
     8941 gaataatacc attattcttt gtagttaaaa ttgcagaatc cagaaatttg tctgattgtt
     9001 caggcaacag cattaatgtt cggtctgatt tattttctat attatgcttt tttatttctg
     9061 aagaatcacc ttctattgat atcacattac ctacaagttt gttaatttca atattcttat
     9121 tacataattt tgccgcctca ggattaatat caatattata gacagtacat tttttcgttt
     9181 ttgctatcaa aatagaaaac attccgatac caccgaacat attaacaaca acctcaccat
     9241 ctttaaccaa accagcaatc ctttcacgtt ccgtagataa acgaggggaa aaaaatgcct
     9301 tttctacatc aacaataaat ttacatccag tctccttgta ttcggtttgt gtattatcaa
     9361 tcccagccaa aatttctaaa cttctagttc taaattcacc ctcaactggt gatgattgat
     9421 aaaatactga tcttgccgtt tttacttgtt ctaatagggt ttcacctatg attttctttt
     9481 ttgcctgtaa cgaatcagga attcttaata ctattatttc accaacctga tcaaacgaag
     9541 aaaataattc catactttct ttttctgaaa gaatattttc taatgctttt tttagcatac
     9601 gtgtcaattt ctgtaataat aattgataga gtttttttgt tgtttatcca atctacttcc
     9661 ttctttattt actctaggcc gcaaactttt ttcatccctt ctaaaagtga tttcaagtga
     9721 tttaagaaat ctgttcatgg cttctgattt aggtaaaggt gaagaaccat gcccatcaat
     9781 acttgaagta ccctcaaaaa tcaccattga atctgagaca agatccatta attgcaaatc
     9841 atgatctata attattgctg atttaccaaa cgaacgtaca aatttttgga gaaattttcc
     9901 aatagctatt ctatcttcaa cgtctaaaaa tgcagatggt tcatccaatg catacacatc
     9961 aactttttgt agtaaacacg aagcaactgc gactttctgt agttcaccac cagataaatt
    10021 ttttatagat ttattataca actttttaat ttttagagga tccactatct gttcctcttc
    10081 ctgactatcc tcaattggac tttgatttgc tttatccaaa actgatacaa cttcaacatc
    10141 aatatcattt gagaggtatt gtggtttata cgcaatcttg agtttttttt ctacagtgcc
    10201 aatatctggt ttttctacac cggcaatcat tttcatcata gtagttttac ccaatgcatt
    10261 agctcccata acaccaagta actgaccttt ttttacaaaa ccaggctcaa ttgttaccga
    10321 aaatgtcgga tattttttta caagctttgg gtaagagatg attttatcac catgttgaaa
    10381 ttcatcttgt gatgttgatg cgtcgaacgt aaacttcttg tcacgaaatc ttacattctc
    10441 atttggaaga aatccatcca aaaatacatt tattccaact ttggttgaca ttatacccga
    10501 tacaattcca tacactgcag gaacaccgta taacacttca atgtaatcac tgagatagtc
    10561 taataatgtt agatcatgtt ctacaaccat cacgctttta ccaagttcag ctaagctatg
    10621 aataactctc gctactcctg ttctttgaaa tacatcatta tatgatgagg gctcatcaaa
    10681 aaaataaaaa tctgcatttt ttgatgctgt aatggctaca gctaatcttt gaagttctcc
    10741 tccacttaat tcttgaagat tttgttctaa tgaatttttc aaatccaatt tttttactaa
    10801 atcatttgta attcctcgct catcatattt tgctaaaagt tcttttcctg taccatcaaa
    10861 agcatttaca atatcatata cttgttgtgg ttttatagag gatttgataa tattttcact
    10921 aatctgttcg aaatgtgatt tgagttctgt tccactatag tatcttagta tttcatccca
    10981 gtcgggtggc tcgttataat ttcctaagtt tggttttaaa tttcctgata gaatattgac
    11041 tatcgtactt ttccccatac catttctacc taacaatcca acaacttcac ctttttttgg
    11101 tgtaggtaat ttgtataatc tgaatgaatt gataccatac tggtggattt tatcagtcga
    11161 aagttctgtt gccaaattaa caatcgtaat agcatcaaat ggacaaactt tcacacaaat
    11221 accgcaccca ttacaaatat cctcatcaat ttgagccctt tttgtttctt catttagtat
    11281 gatacaatca gctcctgatt tattcacagg acagtatttg atacattcta aaccacattt
    11341 ttttggctgg cacaaatctt tgtctaatgc cgctatacga tgagtcatgt caacattgat
    11401 cttatcttta atttatatct cgtgagaaaa tatgaagaac aaaatactca cttttatgat
    11461 tcataacata gttacgttaa tagataagat tgactcatac tcagtaagcc ggtcatagcg
    11521 ttggggtgcg acccagtctc tttccgaacc tggaagtcaa acccaatgtc gctgctatgt
    11581 tactaagatg cgagagctct tgggaagtag tagtgctggc attttattat acaaataatt
    11641 aatagtcata tagatttgga gtttgtgtgt ctacttgttc tgtatgtgga gagaactttt
    11701 ctaacgatat cctgtttcta aatcatatga tggaaaaaca tggatttaga aatccgaata
    11761 tagacaaacc tgatagaaat cagcgaggat actagtttta tcttaattat ttggtatttt
    11821 gatcttttta ctattagtta gaaggcttgc tgaacgaaaa aatactgctg ctgggctttt
    11881 acatctaagg gtattcttct tctgaatttt tttctttgga aaagattcac tactattcaa
    11941 aatcgcagaa aataatggag aaatagctgc ccttccacca taagcagttt tgctatcaat
    12001 aaaccaaaac atctttagtg catttgaatc tagaaaatta tctctgagat cttttccaaa
    12061 atctgtgtaa tgaccaacaa gatatagtct accctttgcc aaataattaa caagtttagc
    12121 aaactgaatg catacataac actcatcatc atagatcatt aatggcacgc tatcctgaat
    12181 cttcaagtca atattcatct accaattaaa attgtcataa ttatatttaa ttcattgaaa
    12241 aaaggttcaa aatatatgca gttatacata tgatacttga tttttattat atgggactga
    12301 attactatca gatcaaaaag caggttctta aggctagaaa acttgtgatt aaggctacag
    12361 ctgctgctgg ttctggtcac ccaggaggat ctttttctat ggctgaaatt ttgggctgtt
    12421 tattttacaa gtatttgaaa tatgatccaa aaaatccagg ctgggaagat cgtgacaagc
    12481 ttatactttc aaagggtcat gcatcacctg gccttttttc aaatatggca gtagctggat
    12541 attttgatcc taaggatatt gtaacattga gacagtttgg aagcaagctt cagggtcatc
    12601 cagatctcaa atgccctgga gttgagtttt gtggtggctc cttgggaatt ggattatcat
    12661 attctatagg taatgcattg gctgcaagac ttgatggtaa gaataataga atttatacaa
    12721 tcattggtga tggcgagtct gatgagggtc aagtttggga ggcagcaatg acagctgcaa
    12781 agtataagat tgataacatt acagcattcc ttgatagaaa tttcatacaa caagattcct
    12841 atacagaaaa aattatgcca ttagatgagg aattaattgg taacgacttg tcagagatgt
    12901 ggaaagatgc aagtagatgg aagactggtg ataaatggcg ttcttttggt tggaatgtta
    12961 tagaggttga tggacataga actgagcaaa tttctgatgc aataagaaga gctaatgaaa
    13021 ccaaaggcct tccatcaata attattgcta
//
LOCUS       DASCPJ010000115         3378 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001799303, whole genome shotgun sequence.
ACCESSION   DASCPJ010000115 DASCPJ010000000
VERSION     DASCPJ010000115.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001799303"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="QGF01_05620"
     CDS             complement(<1..1865)
                     /locus_tag="QGF01_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HJN20394.1"
                     /translation="MRKLFFLIPLLASFLLIPIVNAEGTLGDDWSAIKKTLGDAYKAE
                     TTDESLKLLDDAKAIYESKFATTAKMHDLDTHNVIMACYDDAEAAYKAGDNKQAKLWI
                     QCQEKSIYTLGMVKMEDAISKNNAQEYLDWSTIVQKKFKVADKDPGTLALFTAIENDP
                     SKLKTYSTVIRENMLDIFELKTVEELEEALIKYAEDDTYSAKKYAYEGLYYYRTLDPY
                     VADSVGNAKADKLMNLMIEAMAISDSANDGVSVADLTAQMKDTKKEVEKIVMEHNGIS
                     GTPEALALAGIADRLTLVKLEYVDAIDNSGNVINDMEYAETVAFAGGAVEIADENAEV
                     LSAISSSDFSALQSKLSSIVSNVDSKAAIYIVLSGADDATVIVKNLQLGAGEGGANLG
                     SYFDTIDRLLITAQAAYANGDSDLAFELVSEAYLDNYEFLEAPIAEIDTDLMHKIEDN
                     MREELRNMIKSGSSHNLIEAQILMINNDLATANSLLTGIVPTAEAASAESQPSGGGCL
                     IATATFGSEMAPQVQFLREIRDNTVLQTESGTTFMTGFNQFYYSFSPVIADYERENPV
                     FKETVKLALTPMLTSLALLNYVDIDSEHEMLGYGIGVILLNIGMYFVAPALAIVT"
     gene            complement(1945..>3378)
                     /locus_tag="QGF01_05625"
     CDS             complement(1945..>3378)
                     /locus_tag="QGF01_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HJN20395.1"
                     /translation="PLMIMKGDGGVTSMETFRTKPILTILSGPAASVAGALLYLKVTN
                     GIFVEVGGTSTNICIIKNGKPTIRYVTIKNHPTCIRSMDVRVLGVAGGSMIKIKSNHI
                     DGVGPRSAHIAGLKYSCYADSEDLRSGEIIFVKPKESDIDEYVAIKCKNGTYAITNTC
                     AANALEMIEKDDYSFSNSESAKIALEILGKKLNMSWSMVATSIIQTSSFEITKEISKI
                     IKEFKMETSQIKLVGGGGGASVLVPFVAKQLNLTYEKADFAEVISSIGVASSMMQEQL
                     EQTVTDPNPEQISQLHKKIHSIMIEKGASPESISIDSEYIPEKSVLRVSAIGNVEFDN
                     RVSAKNVFTLEEAKHRASEIIKIPEERIELSFETDHYFVFTGHTKVKKLLGKKNQHHI
                     IILDRFGREKLSVKNGIMFQGGKITVIEQLNNFLANKDDIAPQVYLLNELKLVDFSGL
                     TSKSHILEGTKKELADSIKAAVIIEQK"
BASE COUNT          944 a          639 c          539 g         1256 t
ORIGIN      
        1 attgttacta tagctagtgc tggtgccaca aagtacattc caatgtttag tagaatgaca
       61 cctattccat atccaagcat ctcatgttct gaatcaatat caacatagtt gagcaaggcc
      121 aatgatgtca gcattggagt aagtgctaac tttactgtct ctttgaagac tgggttctct
      181 ctttcataat cagctattac tggactaaat gagtaataga attggttgaa tcctgtcatg
      241 aatgttgttc ctgattcagt ttggagtaca gtgttgtcac ggatctctct caagaattga
      301 acctgtggtg ccatttcaga accaaatgtt gctgtagcaa ttaaacatcc accacctgat
      361 ggttgtgatt ctgcagaagc tgcttcagca gttggtacaa taccagtcag taatgaattt
      421 gcagtggcaa gatcattgtt tatcataagt atttgtgcct caatcaaatt gtgtgatgag
      481 ccacttttga tcatatttct taattcttct ctcatattat cttcgatctt atgcatcaag
      541 tcagtgtcaa tttctgcaat tggagcctct aggaattcat agttatccaa gtatgcttca
      601 ctgactagtt caaatgccaa gtcagagtca ccgtttgcat atgctgcttg tgcagtaatt
      661 agtaatctgt caatagtatc aaagtaactt ccaagattag ctcctccttc acctgcaccc
      721 aattgtaaat tcttaacaat cactgttgca tcatctgctc cacttagtac tatgtatatt
      781 gctgctttgc tatctacatt ggatacaatt gatgataatt ttgattgtag tgcgctaaag
      841 tcgcttgatg atattgcact taatacttct gcattttcat ctgcaatttc tactgctcca
      901 cctgcaaatg ctacagtctc tgcatattcc atatcgttta tgacgtttcc agagttatca
      961 attgcatcaa catattctag tttaacaagt gtgagtctat cagcaattcc tgctaatgca
     1021 agtgcttcag gagtaccgga aattccgtta tgctccataa caattttttc tacttctttc
     1081 ttggtgtctt tcatttgtgc tgttaaatct gctacagata cgccatcatt tgcagaatca
     1141 gaaattgcca ttgcttcaat catgagattc atgagtttgt ctgctttagc atttccaacc
     1201 gagtctgcta catatgggtc tagagttctg taatagtata atccttcata ggcatatttc
     1261 tttgcagaat atgtatcgtc ctctgcatat ttgattaatg cctcttctaa ttcttcaact
     1321 gtttttaatt caaaaatatc aagcatgttt tctctgatga ctgtagagta tgtttttaat
     1381 ttggatggat cattttcaat tgctgtaaat agtgctaatg ttcctgggtc tttgtcagct
     1441 actttgaatt tcttttgtac aatagtggac caatcaagat attcttgagc attgttcttt
     1501 gaaattgcat cctccatctt taccatacca agagtgtaaa ttgatttttc ttgacattga
     1561 atccatagtt ttgcttgttt gttatctcct gctttgtatg ctgcttctgc atcatcataa
     1621 caagccataa ttacattgtg tgtatcaaga tcatgcattt ttgcagtggt tgcaaattta
     1681 ctttcataga ttgcttttgc atcatcaaga agttttagtg attcgtctgt tgtctctgct
     1741 ttgtatgcat caccaagtgt tttcttaatt gcactccaat catcaccaag tgttccctca
     1801 gcattaacaa taggaattag caaaaaagaa gccaacaatg gtatcagaaa aaataatttc
     1861 ctcattctta gaaatcagca gtagattatt tattgtttaa actcattcta tatttttatt
     1921 ctaatattat agaatatgga atgtttattt ttgttctatg attactgcgg ctttgattga
     1981 atccgctagt tcttttttgg ttccttctaa aatatgtgat ttggaagtaa gcccagaaaa
     2041 atctacaagc ttcaattcat ttagtaaata gacttgtggt gcaatatcgt ctttatttgc
     2101 gagaaaatta ttcaactgtt caataaccgt tatcttacca ccctgaaaca ttataccatt
     2161 ttttactgat agtttttctc gtccaaatct atctaaaata ataatatggt gctgattttt
     2221 tttgcctagt aattttttca cttttgtatg accagtaaat acaaaataat gatctgtttc
     2281 aaaacttagc tcaattcgtt cctcaggtat cttaataatt tctgatgctc gatgttttgc
     2341 ttcttcaagt gtaaatacat tcttagccga tacacgatta tcaaactcaa catttccaat
     2401 tgctgaaacc cgaagaactg acttttctgg tatatattca ctatctatgg atattgattc
     2461 gggcgatgca cctttctcaa tcataataga gtgtattttt ttgtgtaact gtgaaatttg
     2521 ttctggattt ggatctgtaa ctgtttgttc taactgttct tgcatcattg atgatgcaac
     2581 acctattgat gaaataacct cagcaaaatc tgctttttca taagtaagat taagttgttt
     2641 tgccacaaaa ggaaccagga cagatgcacc accacctcca ccaactaatt ttatttgtga
     2701 tgtttccatt ttgaattctt ttataatttt actaatttct tttgtaattt cgaacgatga
     2761 tgtttgtatg atagatgttg caaccattga ccatgacata ttgagttttt ttccaagaat
     2821 ttctaatgca attttggcag attctgagtt ggaaaatgaa taatcatctt tttctatcat
     2881 ctcgagtgcg ttggcagcgc aggtattggt aattgcatat gttccatttt tacattttat
     2941 tgcaacatat tcatcaatat cactttcttt aggcttgaca aaaattattt cgccactacg
     3001 aagatcctct gaatctgcat aacaagaata ttttaaacca gcaatgtgtg cacttcgtgg
     3061 tccaacccca tcaatatgat tagatttgat tttgatcata cttcctcctg caactcctaa
     3121 tactcgaacg tccatagaac gaatacatgt aggatgattt tttatggtga cataacgaat
     3181 tgttggcttt ccatttttaa ttatacaaat atttgtacta gttccaccaa cctctacaaa
     3241 tattccattt gtaactttaa gatagagtaa tgctccagca acactcgcgg cagggcctga
     3301 taaaattgtg agtattggct ttgtccgaaa agtttccata cttgtcacac cgccgtcacc
     3361 tttcataatc attaatgg
//
LOCUS       DASCPJ010000116         1646 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001825889, whole genome shotgun sequence.
ACCESSION   DASCPJ010000116 DASCPJ010000000
VERSION     DASCPJ010000116.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001825889"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="QGF01_05630"
     CDS             complement(<1..741)
                     /locus_tag="QGF01_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJN20396.1"
                     /translation="MANFLCRFCNSHLEHVFLDLGKTPSANSYLKQEELLEEKTYSLC
                     VYICEECFLVQIPEILTPDELFSDYAYFSSFSDIWLDHAKKYVEMMLKRFNLNEESYV
                     IEIASNDGYLLKNFVKHKIPVLGIEPASNIAKIATKNGIPTLEKFFNTDCANELKEKN
                     QQADLLLGNNVLAHVPNINNFIAGMKILLKPNGIITMEFPHILQLIKQNQFDTIYHEH
                     FSYFSFFVIQKIFLSKGLTIFDVEEIPTH"
     gene            837..>1646
                     /locus_tag="QGF01_05635"
     CDS             837..>1646
                     /locus_tag="QGF01_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013530.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HJN20397.1"
                     /translation="MGQSSIVVENDIELIVNDIKQFFDIMENKKFLIVGGKGFIGTYF
                     VKVLSRINKSLKKPIKITVIDNLITAKDKDPYQDPNITFLEKDISKEIQYADDLDYII
                     HAASIASPPYYRKHPLETIDVNYQGTRNLLELSKEKKVSGILFLSSSEIYGDPDIIPT
                     PESYQGNVSCTGPRACYDESKRLAETVSILYEQIFKIPVKIARLFNVYGPFLNLDDGR
                     MIPDFMKSAIKDSKIIIHSDGSVTRSFCYITDVISALFKILMIAPPGSICNV"
BASE COUNT          609 a          213 c          244 g          580 t
ORIGIN      
        1 atgtgttgga atttcctcga catcaaaaat tgttaatcct ttagataaga atattttctg
       61 aattacaaaa aatgaaaaat aggaaaagtg ttcatgatat atagtatcaa actgattctg
      121 tttgattaat tgtaatatat gaggaaattc cattgtgatt ataccatttg gtttaagtaa
      181 aattttcatt ccagcaataa aattattaat atttggaaca tgtgctaaaa cattatttcc
      241 taataagagg tcagcttgct gatttttttc ttttaattca tttgcacaat ccgtattgaa
      301 aaatttctct aatgtcggta taccattttt tgtggcaatt tttgctatat tagatgcagg
      361 ttcaatacct aacactggga ttttatgttt gacaaaattt tttaataaat atccatcatt
      421 acttgctatt tctataacat atgattcttc attaagatta aaacgcttga gcatcatttc
      481 aacatatttt ttagcatgat ctaaccagat atctgaaaat gatgaaaaat atgcataatc
      541 tgaaaacaat tcgtccggag tcaaaatttc tggtatttgc accaaaaaac actcttcaca
      601 gatataaaca caaagagaat atgttttttc ttcgagtaat tcttcttgtt ttagatatga
      661 attagctgat ggagtttttc ctaaatctag gaaaacatgt tctagatgtg aattacaaaa
      721 tctacataag aaatttgcca atttctattt agaaaccgca taaattactt aatgtttatg
      781 atttaatgat agcaatcagt aatataatta atataagaaa ttttgatgta tagtaaatgg
      841 gacaatcatc tatagtagtt gaaaatgata ttgaattaat tgtaaatgac atcaagcaat
      901 tttttgatat aatggaaaat aagaaatttt tgattgttgg aggaaaagga tttattggaa
      961 catattttgt aaaggttttg tctagaataa acaaatctct aaaaaaaccc ataaaaatta
     1021 ctgtaattga taatctaatc actgcaaagg ataaagatcc ttatcaagat ccaaacataa
     1081 cttttcttga aaaagatatt tctaaagaaa ttcagtatgc ggatgacttg gactatatta
     1141 ttcatgcagc aagtattgcc tcacctccat attatagaaa acatcctttg gaaacaattg
     1201 atgttaatta tcaaggtact agaaatttat tagaactttc taaggaaaag aaagttagcg
     1261 gaattctatt tcttagtagt agtgaaattt atggtgatcc agatataata ccaacaccag
     1321 aatcgtatca aggaaatgta tcttgtactg gtcctagagc atgttatgac gaatcaaaaa
     1381 gattagcaga aacagtttca atactatatg aacaaatttt taaaatacct gtaaaaattg
     1441 ctcgattgtt caatgtctat ggaccgtttc tgaatctaga tgatggaaga atgattccag
     1501 attttatgaa aagtgccata aaagattcaa aaataattat tcatagcgat ggttcagtaa
     1561 caagaagttt ttgctatatc actgatgtaa tatctgcttt gtttaaaata ttgatgatag
     1621 caccaccagg ttcaatatgt aatgtt
//
LOCUS       DASCPJ010000117        14773 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001830479, whole genome shotgun sequence.
ACCESSION   DASCPJ010000117 DASCPJ010000000
VERSION     DASCPJ010000117.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 14773)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14773)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14773
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001830479"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2126
                     /locus_tag="QGF01_05640"
     CDS             <1..2126
                     /locus_tag="QGF01_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HJN20398.1"
                     /translation="DARRAFPCWDEPSVKSTFDVSLLVDKHLDAISNMPVSSKKTVDS
                     KFLYEFQRTPIMSTYLLYLGVGEFEYLHGKLRDIKLRIVTTKGNKNKGKLSLQFTKQF
                     LAEYEKYFGIKYPLPKLDMIAIPDFAAGAMENWGAITFREAILLYDPKTSSTKTKQYI
                     AEVISHELAHQWFGNLVTMKWWNDLWLNESFATFMATKIVDKFYPEWDLWNQFLEDAM
                     LQAMGLDALKNSHPINVDVKHPAQIREIFDVISYDKGGCVLRMLENYVGVENFRKGLK
                     HYLTKYRYSNAEGQDLWSSIGGIAHKPVKPMMKTWIDKVGYPIVTAQRENSHVLLSQK
                     RFLADGSKSSKNEWEIPITIDEGNHQNSILLKSHHRKISLKNTDSNFIINSGRYGFYR
                     VEYDTHTLANLSLLVDEKILNHVDRWSLQNDLFSQCVTGVKQLQEYLDFTTSYHDEDD
                     YITLLNVAHNLYYLYNLTINEKFSDEIRVYALQFLGSIFDHLGWDSKKNEKHTDSLLR
                     SFVITALGKLGDKEILNESKKRFNKFIKNKNSLAADLQEPVFILTAWQGDQKTHSKLI
                     SLYKKAILQEEKMRFLTALCNFKQKNLLIKTLNFSLTSDVRSQNIRVPIMNIASNVHG
                     KDILWPWLKKYWRNLVKKFGVGNPLANRIVASIGGVVEYKQEKEVRAFFKRNPMPGTE
                     RILEQTLERVRIRSKFLQQIKKEFI"
     gene            2129..2536
                     /locus_tag="QGF01_05645"
     CDS             2129..2536
                     /locus_tag="QGF01_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20399.1"
                     /translation="MIKKLVIGACLSVIAIIMVLAISNSPIDESMISQTISVDALYKP
                     ENNTIEITYVDVSEKTDSVIIEILGMDETFHKEFSQQSFVETVQIISPPKYGWATMPV
                     TFSIIHNQFGEIGLKIEIHEQDEPKSRIIYMKT"
     gene            3211..4128
                     /gene="meaB"
                     /locus_tag="QGF01_05650"
     CDS             3211..4128
                     /gene="meaB"
                     /locus_tag="QGF01_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HJN20400.1"
                     /translation="MDITSDLKKAKRGAIAKAISIFENDKKESRKLIKKIFKSSGKSI
                     VIGITGAAGAGKSSLIDKTSIQLKKLGMKPAILAIDPTSHVSGGAILGDRVRMMESTD
                     SGTYIRSIASRGATGAISRSIRNSIRVLEYAGFNPIIIESVGAGQTEIEISHIADITV
                     VVFNPHTGDSIQTIKAGITEIGDIYIVHKSDLDGASQLFQSVQDFIGSVEKNPVILKA
                     SSKTGKGVAEFIKELKILIANKKKEKKLSEKQRLTKELDDIILDNINEKISSVLHSNK
                     SYSSYLKKVHEKKIDPYEAAENISKSILK"
     gene            4134..5723
                     /locus_tag="QGF01_05655"
     CDS             4134..5723
                     /locus_tag="QGF01_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HJN20401.1"
                     /translation="MTKEKPKSKQFVTDSNISVKPAYYGSTKKPKKEEPGKYPFTRGI
                     HPGMYRDRLWTMRQYSGFGDAVQTNKRYRFMLENGQTGLSMAFDLPTQIGYDPDSPQA
                     EGEIGKVGVSIASLKDMQTAFNKINLGEVSTSMTINATASTLLAYYIAVGKSQGISSK
                     ELRGTTQNDILKEYVARNTYIYPPKPSMRLIGDMIGYCAKNVPQWYPISISGYHMREA
                     GSTAIQEIAFTIANAIEYIQTCIDRGLKIDDFAPRLSFFFCCTIEFFEEVAKFRVARK
                     VYAKILKERFHAKDPKSLHLRFHTQTSGESLTAQQPDNNIVRVAIQAMAAVIGGTQSL
                     HTNSKDEALALPTQEAAKIALRTQQIIGFESGITKTVDPLAGSYYMESLCDEIEEKTW
                     AYLKKIDKMGGALKAIEKGFFQSEIRQNAYRLKKEVDNNERVLVGVNKFDDKSNKKQD
                     LLRVTDSLGKKQEKAIKELRKSRDEKKTQYALSKMQKAAESDKNLMPFIVDAVQAYAT
                     TGEISNTFREVFGQYRPREVF"
     gene            5725..6120
                     /gene="mce"
                     /locus_tag="QGF01_05660"
     CDS             5725..6120
                     /gene="mce"
                     /locus_tag="QGF01_05660"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HJN20402.1"
                     /translation="MKIDHIAIVVNNVEEATKTYQEALDVKEVEFETVESEGVKVAIL
                     HLENANIELMEPTTDSSPIKKFLEKRGNGLHHIALETKNIEDEVTRMEGCGIQFLGKI
                     RPGSAGTKVTFIHPKSLNGVLTELCSKQN"
     gene            6125..6373
                     /locus_tag="QGF01_05665"
     CDS             6125..6373
                     /locus_tag="QGF01_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20403.1"
                     /translation="MYKKLILHSLFFFLISSVVLSPVFAELDFESGPLDQKPLDEREQ
                     EDQDVMDVWIYAIIIVIIMLIARLGFRVIKKRRTKKKD"
     gene            complement(6370..6933)
                     /locus_tag="QGF01_05670"
     CDS             complement(6370..6933)
                     /locus_tag="QGF01_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20404.1"
                     /translation="MELDVDEMKVLGAIVGGGKTIKNIKQTSRLDKKEIEIILESLDR
                     YEFISGVEGKGMWGQTQFYFTPTDDGSKKVDEYIEHLKSEWKKIIEFATDGKRDQLDD
                     YMAQHKFLVNMMLYFKIVNLPALGRLNLRFLIEGKHLCYKCKKELGRFALKFSVSDCR
                     KRGLKMPKGLTTHDDLCADCFDGLSVG"
     gene            7016..7261
                     /locus_tag="QGF01_05675"
     CDS             7016..7261
                     /locus_tag="QGF01_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20405.1"
                     /translation="MDSCSRHTAYRHGKTYEECKELAENFTAELKPEIQTKGRLLWAD
                     LLEKVEHDELVYKLTLKYLRRDGFDIGNNKIPEIKKT"
     gene            7343..9361
                     /locus_tag="QGF01_05680"
     CDS             7343..9361
                     /locus_tag="QGF01_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HJN20406.1"
                     /translation="MVLLLKRTPVLLLGSMLVIATLFAMLPDAAADVDAGVGEFRLGE
                     VLDESIGWFIVVGLGAIFAGVISAEIKLEEKYLGHVQNSEWFNTAGRIIKTGLTAAAI
                     VSAWTWAATLLQSSTVAYLYGISGPFWYASGATIQVLLFGILAIELKRKAPNAHTFLE
                     IIRARFGNGTHKIFLGFALTCNMIVTGMLLLGGAAVVNGLTGMNISIAAFLIPVGVMV
                     YTLVGGLKATFVADYMHTVIIYVVILTFVSMVFFISPVTGGIEGMYDKLAAAADLRPV
                     LEPTFTPGEPGNAMGAYLTMASAGGLIFGVINIVGNFGTVFVDQAYWQRAIAAQPSST
                     VKGFLLGGMCWFSIPFTLATTMGLTAVALNVQLTPEQVQLGLTVPAAASVMMGEVGAI
                     MVLVMLFMAVTSAGSAELIAVSSLITYDVYRTYSNPAATGKQLLKVSRTVIVIFGLGM
                     GALAVVLLGMGLSLGFVYLAMGILIGSAVIPIALTITWSKTTRAGAVAGALIGVMLSL
                     LTWTSVAASEANGVIDISSLGGAFPMLYGNVVAILSSGLICVVISLAQNKKYDWSQLN
                     THMKIVEGDMSEDAKQAIAQAEQDEATLKKAYQFSLKGGGILTIICVVIWPLPLYFSG
                     YVFDFGFYSMWVGIAIVWVSIAAFCIICIPIYEARAGFAKVARGGSAA"
     gene            9400..9891
                     /locus_tag="QGF01_05685"
     CDS             9400..9891
                     /locus_tag="QGF01_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20407.1"
                     /translation="MASCNGCGEALGFKKYRFNKLWKIKGYYCKPCMMKVGENWEKYG
                     RVTIPMVPCDLCRTEFYFLKPAWQGEKERSFCGVCYRTAISGVLPDKNKGEAPGKMPI
                     SMMIFAGLGALMMVLGMVFTLVGTAAGDANMVNILFGAFTTAAGFMLLRRTLRNRRLI
                     AGA"
     gene            complement(9888..12011)
                     /gene="ureC"
                     /locus_tag="QGF01_05690"
     CDS             complement(9888..12011)
                     /gene="ureC"
                     /locus_tag="QGF01_05690"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HJN20408.1"
                     /translation="MLARWHSDTPHVGEYIPTDESIKANKDKKTISLNVSNTGDRPIQ
                     VGSHTHFSEANKALEFDREKSLGFHLNIPSGTSIRFEPGESKRVEVVEFGGTKTIFGF
                     SGLVSGDLNSKKQDAIKKINENGFKNISEDVKEGSNSLEIQRSRYVALFGPTVGDRVR
                     LADTDLIIEIEKDLIKYGDELVFGGGKSARDGLGQASGVLRENSADLVITNAMIIDPV
                     LGIIKADIGIKDGKIIGVGNAGNPNVMDDIDITVSSNTEIISGEHTICTPGTIDSHIH
                     FISPQQAIDAICNGTTTMIGGGTGPADGTNATTCTPGEWNIHKMIEAVEEYPLNFGFL
                     CKGNDSREEALLEQVKAGACGLKLHEDWGTTPATINSALNVADKTDTQVAIHTDTLNE
                     CGYVDDTIKAIAGRTIHTYHTEGAGGGHAPDIMKIAGEPNILPSSTNPTRPYTVNTLE
                     EHLDMMMVCHHLNPSVPEDVSFAESRIRAETIAAEDVLHDIGAISMMSSDSQAMGRVG
                     EVTTRNWQTADKMKKMKGSLSEETGDNDNLRIKRYMAKITINPAITHGISSYVGSLES
                     GKMADIVVWSPQFFGIKPKLIIKGGFIAYALMGDPNASIPTTEPVYYRPMFGALGKAK
                     QTTSVTFTSQIALDNKLEDKLKIQKKLVAVKNCRGIGKSDMLYNDKTPKIEVDPETYE
                     VKVDGKIATVDPAEKLSLARLYSLY"
     gene            complement(12017..12319)
                     /locus_tag="QGF01_05695"
     CDS             complement(12017..12319)
                     /locus_tag="QGF01_05695"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HJN20409.1"
                     /translation="MNLTPRELDKLHVFMIAELARKRKARGLKLNHPESVAIIADHIL
                     EGARDGKSVSDLMSSGKKVLGKDDVLPGVTDIIHTIQVEATFEDGTKLVTVHDPIV"
     gene            12652..13164
                     /locus_tag="QGF01_05700"
     CDS             12652..13164
                     /locus_tag="QGF01_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Urease accessory protein"
                     /protein_id="HJN20410.1"
                     /translation="MNKHSMLIVNSILGNIHNDNLLENKVDDARQQGKLRHLLLSRIE
                     MEKSRLRKKTEDGFDIGLVLDPGKKLHHGDVISQDSEIILIDQLPEKILTVRFIENDK
                     RLFLLVGHIIGNRHRPISIKNETISFPIHDDSEMELFGKLFHDVINKIDLSMQEEIFI
                     PHESMDVHEH"
     gene            13136..13846
                     /locus_tag="QGF01_05705"
     CDS             13136..13846
                     /locus_tag="QGF01_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HJN20411.1"
                     /translation="MKVWMYMNTDFSDLSLLQLSDSFFPTGLYTMSNGLETVFSEKTI
                     TSIDDLYELIKTNIEQQIGPADCVALGCAYDFTSLNNVEKIIMCDTSLFAMKLIGELR
                     EASCRSGTQMMKCVGSFVSNNTLSEYHNAIKESHSPGTHAVVTGVVSNILGIKKQKSV
                     LHFLYGFSVSMIGAALRLGMIEHFQSQKILHQLKPVITETCNEYITKSLDDMWQFAPE
                     YDIIQIEHEQQFSKMFIT"
     gene            13861..14520
                     /gene="ureG"
                     /locus_tag="QGF01_05710"
     CDS             13861..14520
                     /gene="ureG"
                     /locus_tag="QGF01_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HJN20412.1"
                     /translation="MNKRIPRIGIGGPVGCGKSMLIERVVPILAKKNYQISIISNDVI
                     SQEDADRMRQNLATDQGLLPENLVIGLATGGCPHTAVREDPSINLSVIEQLESEHTDL
                     DLIIIESGGDNITTTFSPALADYFIYIIDVSGGDKYPRKRGLGIETADLLVINKIDLA
                     ELVNANLDVMERDAKIVRKNKPYALVNCKTDQGIDTVVQHIIEDVLFDQPPKKMVTSV
                     K"
     gene            14517..>14773
                     /locus_tag="QGF01_05715"
     CDS             14517..>14773
                     /locus_tag="QGF01_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HJN20413.1"
                     /translation="MTDLKFCESDDVPAIFSAYDKKIEQLDVGKSGKVGLLRLKLEEN
                     SDKNKTIITDQYSQVPLYTQKALYYDEKLPNMAHLFIMSPSG"
BASE COUNT         4981 a         2422 c         2747 g         4623 t
ORIGIN      
        1 ctgacgctag aagagcattt ccatgttggg atgaaccatc tgttaaatct acttttgatg
       61 tttcattatt agtggataaa catcttgatg caatttcaaa tatgcctgta tcatctaaga
      121 aaactgttga ctctaaattt ttgtacgagt ttcagcgaac cccaataatg tcaacatact
      181 tactctatct tggagtgggt gaattcgagt atttgcacgg aaaattgcgt gatataaaac
      241 tacgaattgt taccacaaag ggtaataaga ataaaggaaa actttcatta cagtttacaa
      301 aacaatttct tgctgaatat gaaaaatatt ttggaataaa atatccactt cccaaacttg
      361 acatgattgc aattcctgat tttgctgctg gtgcaatgga aaattggggt gcaattacat
      421 ttagagaggc gattttactt tacgatccaa agacatcctc aactaagaca aagcagtaca
      481 ttgcagaggt aatatcacat gaattagcac atcaatggtt tggtaatctt gtcacaatga
      541 agtggtggaa tgacctttgg ttgaatgaga gttttgcaac atttatggca acaaagattg
      601 ttgataaatt ttatccagaa tgggatcttt ggaaccaatt tttggaggat gcgatgttgc
      661 aagccatggg cttagatgca ttaaaaaatt ctcatcccat caatgtagat gtaaagcatc
      721 ctgcacaaat tagagagata ttcgatgtaa ttagttatga taaaggtgga tgtgttttac
      781 gtatgcttga aaattacgtt ggtgtggaaa actttcgaaa aggactgaaa cattatctca
      841 caaaatatag gtattcaaat gctgaagggc aagatttatg gagttctatt ggaggaattg
      901 cacacaaacc cgtcaaacca atgatgaaaa catggataga caaagttggt tatcctattg
      961 ttactgctca acgagaaaac tcacatgtct tactatcaca aaaacgattt ttagcagatg
     1021 gcagtaaatc ctcaaaaaat gaatgggaga ttcctataac gatcgatgaa ggaaaccatc
     1081 aaaactcaat actgttaaaa tcacatcata gaaaaatatc attaaaaaat actgattcaa
     1141 actttataat taattctggt cggtatggat tttacagggt agaatatgat acacatactt
     1201 tggcaaatct gtcattattg gtagatgaaa aaatactgaa tcatgttgat cgatggagtt
     1261 tacaaaatga tttgttttca cagtgtgtta caggtgtcaa acagcttcaa gaatatcttg
     1321 attttaccac atcatatcat gatgaagatg attacattac tttgcttaat gtggcacaca
     1381 atctttacta tctgtataat ctcacaatta atgaaaaatt ctctgatgaa attcgtgttt
     1441 atgccttaca atttttggga agtatatttg atcaccttgg atgggattct aagaagaatg
     1501 aaaaacatac tgattcatta ctacgcagtt ttgtgataac tgcacttgga aaattaggtg
     1561 ataaagaaat actcaacgaa tctaaaaaac ggtttaacaa atttatcaaa aataaaaatt
     1621 cattggctgc tgatttacag gaaccagttt ttattttgac agcgtggcag ggagatcaga
     1681 aaactcatag taaactaata tcactttaca aaaaggctat tcttcaggaa gaaaaaatgc
     1741 ggtttcttac cgcattgtgt aattttaagc agaaaaatct tctcataaaa actctaaact
     1801 tttctttgac ttcagatgta cgttcacaaa atatccgtgt accaattatg aatatagcat
     1861 ctaatgttca tggaaaagat attctctggc cgtggctaaa aaaatattgg agaaatcttg
     1921 tgaaaaaatt tggagttgga aatccactag ccaatagaat tgttgcaagt attggtggag
     1981 tggttgagta taagcaggaa aaagaagtac gcgctttttt taaaagaaat ccaatgccag
     2041 gaactgaacg tatattggaa caaacattag aaagagttag aataagatcc aaatttttgc
     2101 aacagattaa aaaagaattc atataactat gattaaaaaa ttagttatcg gtgcatgttt
     2161 atctgttata gcaatcataa tggttttagc aatatctaat tcaccaattg acgaatcaat
     2221 gatatcacag acaatttctg tagatgcttt gtataaacca gaaaataata ctatagagat
     2281 tacttatgtc gatgtatcag aaaaaaccga ttctgtcata attgagattt tgggtatgga
     2341 tgaaacattt cataaagaat tttcacagca gtcatttgta gaaactgttc aaattatttc
     2401 tccaccaaaa tatggttggg caacaatgcc agtaacattt tctataattc acaatcaatt
     2461 tggagaaatt ggtttaaaaa tagaaatcca tgaacaagat gaaccaaaat ctagaataat
     2521 ttacatgaaa acttagaaac caacagatct atatatcatc caacccaaat ttatacatgc
     2581 aaaagggacc tagtcaatgg tgggatgggc ttggcagaga ccttgaggaa gatattgagg
     2641 caaattcctg agtattttag tacaaatcta tatcaacaca acatttttgc tagttaaatt
     2701 aattttcgtg cttcataatt cggcgttcaa agacaaacct tgaactttat ctcatcaatc
     2761 tcttcggaag tcagagagaa ccttcttgaa taatcatcag cactggatta tcataataag
     2821 ggcatttagg ctttccagtt agttaaaaaa tagtacagtt aggatctact aattttacct
     2881 aggtatctat aaggcaagat tatcagcata gtcatgaaat acagatgtaa aacgtgcaat
     2941 actacttgta ctgatataat ggaccatgtt agaaaagttc ataaattttc aaactggata
     3001 atagaagaaa ataccaaaac aaacaagaat agcttcaaaa gtgcttttga agaaataaag
     3061 tagaacaatt taggataact tggtcagtgt ttggatggtc gattttccta gaaataccaa
     3121 ttttcatagt attctaagta ttctaggcat tattccaagc taattggcaa caataccaat
     3181 atttattgca gaaacaaaag ataactatac ttggatatca cttctgattt gaaaaaagca
     3241 aaacgtggag caattgcaaa ggcaatttct atttttgaaa atgacaaaaa agaatcaaga
     3301 aaattaatca aaaaaatatt caaaagttca ggtaaatcta ttgtgattgg tattacagga
     3361 gcagctggtg cagggaaatc atcattaatt gataaaacat caattcaatt aaaaaaatta
     3421 ggtatgaaac cagctatctt agcaatagat cctaccagtc atgtatctgg gggtgcaatc
     3481 ctaggtgata gagtacgaat gatggaatca acagattcgg ggacatatat tagaagtatc
     3541 gcatcaagag gtgcaactgg agccatttct agatcaattc gaaatagtat acgtgtatta
     3601 gaatatgctg gatttaatcc aataataata gaaagtgttg gtgcgggtca aacagaaata
     3661 gaaatttctc acatcgcaga tatcacagtt gtagtcttca atccgcatac tggtgatagt
     3721 atacaaacca taaaagctgg gataactgaa attggtgata tttacattgt acataaaagc
     3781 gacctagatg gcgcatcaca attattccag tcagttcagg attttattgg aagtgtagaa
     3841 aaaaacccag taattctaaa agcatcatct aagacaggaa agggagttgc agaatttata
     3901 aaagaattaa aaatattaat agcaaacaag aaaaaggaga aaaaattgtc agaaaaacaa
     3961 aggcttacca aagaactcga tgatataatt ttggacaata tcaatgaaaa aatttcatca
     4021 gtactacatt cgaataaatc ctattcatca tatcttaaaa aagtacatga aaaaaaaatt
     4081 gatccatatg aagctgctga aaacatatca aaaagtatac tgaagtgatg ataatgacaa
     4141 aagaaaaacc aaaatcaaaa caatttgtga ctgattctaa catctcagta aaaccagctt
     4201 attatggttc aacaaaaaaa cccaaaaaag aagaacctgg aaaataccca tttacgcgag
     4261 gaattcatcc tgggatgtac cgtgatagat tatggaccat gcgtcaatac tctggatttg
     4321 gagatgctgt gcagaccaac aagcgttata gattcatgct agaaaacgga caaactggtc
     4381 tttctatggc ttttgattta cctacacaaa ttggatatga tccagattca ccccaagctg
     4441 aaggtgagat tgggaaagta ggtgtatcaa ttgcatcatt aaaagatatg caaacggcat
     4501 tcaacaaaat caatttagga gaggtttcaa cttcaatgac aataaatgca actgcatcaa
     4561 cacttctagc atattatatt gcagtaggaa aatcccaagg aatttcaagc aaagaactca
     4621 gaggtacaac tcaaaatgat atattgaaag aatatgttgc tagaaatacc tacatttatc
     4681 ctccgaagcc atcaatgagg ctgataggtg atatgattgg ttactgcgca aaaaatgtgc
     4741 cacaatggta tccaatctca atctcaggat atcatatgcg tgaagcaggt tcaactgcca
     4801 tccaagaaat tgcatttacc attgcaaatg caattgaata tattcaaaca tgtatagata
     4861 gagggctaaa gattgatgat tttgcaccaa gactttcatt tttcttttgt tgtacaatag
     4921 agtttttcga agaagttgca aagtttagag ttgcacggaa agtttatgca aaaatcctaa
     4981 aggaaagatt tcatgctaaa gatcctaaat cattgcatct gagatttcat actcaaacaa
     5041 gtggagaatc attaacagct cagcaacctg ataataatat tgttcgtgtt gcaatacaag
     5101 caatggcagc agttatcggt ggaacacaat ctttgcatac caattcaaag gatgaggcac
     5161 tagcacttcc aacgcaagag gcggcaaaga ttgcattacg aacacagcag attattggtt
     5221 ttgaaagtgg gattactaaa actgtagacc cattagctgg atcctactat atggaatcac
     5281 tctgtgacga gattgaagag aagacatggg cttatctcaa aaagattgac aaaatgggag
     5341 gtgcactaaa agccattgaa aagggatttt tccaatctga gattagacag aatgcatatc
     5401 ggctcaaaaa ggaggttgat aataatgaac gtgtacttgt tggtgttaac aaattcgatg
     5461 ataaatctaa taaaaagcaa gacctcttga gagtaactga ttctttagga aaaaaacagg
     5521 aaaaagcaat aaaagaactt cgtaaatcac gcgatgagaa aaaaacacaa tatgccttgt
     5581 caaaaatgca aaaagcagct gaatctgata aaaatttgat gccgtttatt gtagacgcag
     5641 ttcaggcata tgccacaaca ggggaaatta gtaacacatt cagagaagtt tttggccaat
     5701 ataggccaag agaggtgttt tgatttgaaa attgatcata ttgccattgt tgtgaataat
     5761 gttgaggagg ctactaaaac ataccaggag gcattagatg tgaaagaggt cgagtttgaa
     5821 actgtggaat cagaaggggt aaaagttgca atacttcatc tggaaaacgc aaatatcgaa
     5881 ttgatggagc caacaaccga ttcaagccca attaaaaaat ttttagaaaa acgtggaaac
     5941 ggtttacatc atatagcact tgaaaccaaa aatattgagg atgaagtaac tagaatggaa
     6001 ggatgtggaa tacaattttt aggaaaaatc agacccggct ctgcaggaac aaaggttaca
     6061 ttcattcacc caaaatcatt aaatggtgtt ttaaccgagc tttgcagcaa acaaaattaa
     6121 agatatgtac aaaaaactaa ttctacattc attatttttt ttcttaatat ctagtgttgt
     6181 attatccccc gtatttgctg aactagattt tgagtcagga ccactggacc aaaaaccact
     6241 ggatgaaaga gagcaagaag atcaagatgt tatggacgtt tggatatatg caataattat
     6301 agttataatt atgctaattg ctagattagg ttttagagtt atcaaaaaac gaagaactaa
     6361 gaaaaaagat taacctactg acagtccgtc aaaacaatct gcacataaat catcatgtgt
     6421 tgttaatcct tttggcattt ttaatccacg ttttctacaa tctgaaactg aaaattttag
     6481 agcaaatctt cctaactctt ttttacattt atagcaaaga tgcttccctt caataaggaa
     6541 tcttagattt agtcttccca gagcaggtag attaacgatt ttaaaatata gcatcatatt
     6601 tacaagaaat ttgtgttgag ccatgtaatc atcaagttgg tcccttttac catcagttgc
     6661 aaattcaatg atttttttcc attcactttt caaatgttct atatattcat caaccttttt
     6721 ggatccatca tcagtaggtg taaagtaaaa ttgtgtttgt ccccacatcc ctttaccttc
     6781 aacaccactg atgaattcat aacggtccaa agactctaaa attatttcta tctctttttt
     6841 atcaagtctg cttgtttgct ttatgttttt gattgttttt cctccaccta caatagcacc
     6901 taaaaccttc atttcatcaa catctaattc cataaattct ttgcaaaatt cccattttaa
     6961 aacttgacta gtgtatacga taatgatttt tagattagta aatatgatta aacatatgga
     7021 tagttgttct agacatactg catatagaca cggtaagaca tatgaagaat gtaaggaact
     7081 ggctgagaat tttacagcag aattgaaacc tgaaattcaa acgaagggca gattgctttg
     7141 ggctgatctg ctggagaaag tagaacatga tgagctggtt tacaaactga cactcaaata
     7201 tcttagacga gacggatttg atataggaaa taacaagatt ccagaaatta aaaaaaccta
     7261 gtgccaaaaa aaattttttt ttagcaacgt actaggggtt aataaattga ttgttggcac
     7321 tatagttata tacatctttt ttgtggtact tttgttgaaa cgtacaccag ttttactgtt
     7381 aggtagtatg cttgttattg caactctatt tgctatgttg cctgacgctg ctgctgatgt
     7441 agatgctggt gttggtgaat ttagactcgg tgaggtttta gatgaatcaa taggctggtt
     7501 tatcgttgta ggtctaggtg caatatttgc aggtgtaatt tcggctgaaa ttaaacttga
     7561 ggaaaaatat ctaggtcacg ttcaaaactc agagtggttt aacactgctg gcagaattat
     7621 taaaacaggt ttaactgcag cagcaatcgt atctgcctgg acttgggcag caacactact
     7681 tcaatcttca acagttgcgt atctttacgg tataagtgga ccattttggt atgcatcagg
     7741 agcaacaatt caggttcttt tattcggaat tttggcaatt gagctcaaac gaaaggcacc
     7801 aaacgctcac actttcttag aaattatcag agcaagattt ggtaacggca ctcacaaaat
     7861 tttcttaggt tttgcattaa catgtaacat gattgtaact ggtatgcttc tacttggagg
     7921 cgcagcagtt gtaaatggct taactggtat gaatatcagt attgcagcat ttttgattcc
     7981 agtaggcgtc atggtttata cactcgtagg cggtctaaag gcaacattcg ttgcagatta
     8041 catgcacact gtaatcattt acgtcgtaat tcttaccttc gtttctatgg tattcttcat
     8101 cagcccagtt actggcggta ttgaaggaat gtatgacaaa cttgcagcag cagctgactt
     8161 gagaccagtt cttgagccaa ctttcactcc tggtgaacca ggtaacgcaa tgggtgcata
     8221 cctcacaatg gcttctgctg gtggcttaat cttcggtgtt atcaatattg taggaaactt
     8281 tggaactgtc tttgtagacc aggcttactg gcaacgtgca atcgctgcac agccaagctc
     8341 cactgtaaaa ggattcttac taggaggaat gtgctggttc tctattccat ttactctagc
     8401 aacaactatg ggcttaactg ctgtagcgct taatgtacaa cttacgccag agcaggtaca
     8461 gctcggactt acagtcccag cagccgcatc agtaatgatg ggtgaagttg gagcaatcat
     8521 ggttttggtt atgttattca tggcagtaac ttcagcagga tctgctgaat taattgcagt
     8581 atcttcattg attacttatg atgtatacag aacgtatagt aatccagcag caactggaaa
     8641 gcaactattg aaagtttcac gtacggtgat tgtgatattt gggcttggta tgggtgcgct
     8701 agctgttgtc ctacttggta tgggattgag cttaggattt gtatacttag caatgggcat
     8761 cttaatcggc tctgcggtca ttccaatcgc attaacaatt acttggagca aaactactcg
     8821 tgccggcgca gttgctggtg cacttatcgg tgttatgtta tcattactca catggacaag
     8881 tgttgcagca tcagaagcaa acggtgtaat tgatatatct tcacttggtg gcgcattccc
     8941 aatgttatat ggtaacgttg tagcaatctt atcatctgga ttaatttgtg tagtaatcag
     9001 tttggctcag aacaagaaat atgattggtc acaattgaac actcatatga aaattgttga
     9061 aggcgacatg tctgaagatg ccaagcaagc aattgcccaa gccgaacagg atgaagcgac
     9121 attaaagaag gcatatcaat tcagccttaa gggtggcggt attttgacta taatctgtgt
     9181 agtaatttgg ccattaccat tatacttctc tggttatgtc tttgactttg gattctattc
     9241 catgtgggta ggcatcgcaa tcgtttgggt atcaattgca gcattctgca tcatctgtat
     9301 accaatatac gaggcaagag caggattcgc caaagttgcc cgcggtggca gtgcagcata
     9361 aaatagtaag attataaata agattattta ttttgtatta tggcttcgtg taatggttgt
     9421 ggtgaagcat taggctttaa gaaatataga ttcaataaat tgtggaaaat taaaggctat
     9481 tactgtaagc cttgtatgat gaaggttggt gagaactggg aaaaatatgg aagagttaca
     9541 attccaatgg ttccttgtga tttgtgcaga actgaatttt attttctaaa acctgcatgg
     9601 caaggtgaga aggaaaggag tttttgtggc gtatgctata gaactgcaat tagtggagtt
     9661 ttacctgata aaaataaggg tgaggcacct gggaagatgc ctatttctat gatgatcttt
     9721 gcaggtttgg gtgcattaat gatggtactt ggaatggtgt ttacattggt tggaacggca
     9781 gccggtgatg caaacatggt gaatatttta tttggtgcat ttacaaccgc agccggcttt
     9841 atgttattaa gacgtacttt gcgtaataga cgtttgattg caggagccta atacaaacta
     9901 taaagtctgg caagtgacaa tttttccgca ggatcaactg ttgcaatttt accatcaact
     9961 ttgacttcat atgtttctgg atcaacttct atcttaggcg ttttatcatt atacagcatg
    10021 tcactttttc caataccacg gcagtttttt acagcaacaa gttttttctg aatttttagt
    10081 ttatcttcta atttattatc taatgcaatt tgagacgtaa atgtgactga tgttgtttgc
    10141 tttgctttac caagtgcacc aaacatagga cgatagtaaa caggttctgt tgtaggtata
    10201 gatgcattag gatcacccat caatgcatat gcgatgaatc cacctttgat tatcagtttt
    10261 ggttttattc caaagaactg tggtgaccag acaacaatgt cagccatttt accagattca
    10321 agtgaaccca cataggaaga aattccatga gtgatggctg ggtttatagt aatttttgcc
    10381 atgtaacgct ttattctaag attatcatta tctcccgttt cttcagacaa acttcctttc
    10441 atcttcttca ttttatcagc agtttgccag tttctggtag tgacttcacc taccctgccc
    10501 attgcctgac tatcagatga catcatgctt atggcaccga tatcatgtaa tacatcctca
    10561 gcagcgattg tttcagcacg tattcgtgat tcagcaaaag atacgtcttc tggcacagat
    10621 gggttaagat gatgacaaac catcatcata tcaagatgtt cttcaagtgt gtttactgta
    10681 tatggtcttg taggatttgt tgatgatggt aggatatttg gctcaccagc aattttcata
    10741 atatcaggtg catgtccacc acctgcgcct tctgtatgat atgtatgaat tgttcttcca
    10801 gcaattgctt tgatggtatc atcaacataa ccacactcat tgagagtatc tgtatgaatt
    10861 gcaacttgag tatcagtttt atcagcaaca ttcaatgcag agtttattgt ggcaggagtt
    10921 gttccccaat cttcatgaag ttttaatcca catgcaccag ccttaacttg ttccaataac
    10981 gcctcttctc gagagtcatt ccctttacac aaaaatccaa agtttaatgg gtattcttct
    11041 acggcttcaa tcattttgtg tatgttccat tctccaggtg tacaagttgt tgcatttgta
    11101 ccatcagctg gtccagtacc gccaccaatc atagttgttg ttccgttaca aattgcatca
    11161 attgcctgtt gaggtgatat aaaatgaata tgactatcaa ttgttccagg agtacaaatt
    11221 gtatgctctc cagaaattat ttcagtattt gatgaaacag taatatcaat atcatccata
    11281 acatttggat tccctgcatt accaacacca ataatttttc catcttttat gccaatgtct
    11341 gcctttatga taccaagtac aggatcaatt atcatagcat tagtaattac caaatcggca
    11401 gagttttcac gtaacacacc actagcctgc cccaatccat ctcttgcact tttaccacct
    11461 ccaaaaacta attcatcacc atatttgatc aaatcttttt caatttctat aataagatct
    11521 gtatctgcca atctaactct gtcaccaact gttggtccaa ataatgcaac atatcggctg
    11581 cgttgaatct caagtgaatt tgagccttct tttacatcct cagaaatatt tttgaaacca
    11641 ttttcattga tttttttaat tgcatcttgt ttctttgaat tcaaatcccc acttaccaat
    11701 ccactaaaac caaagattgt tttggtacca ccaaattcta caacttcaac acgttttgat
    11761 tctcctggtt caaatctgat agatgtgcca gatggaatat ttaggtgaaa tccaagtgat
    11821 ttttctctat caaattctaa tgctttgttt gcttcagaga aatgtgtatg agatcctact
    11881 tgaattggtc tatctccagt gtttgaaaca tttaggctga ttgttttttt gtctttgttt
    11941 gccttgatac tttcatctgt tggtatatat tcaccaacat gaggtgtgtc ggaatgccat
    12001 cttgctaaca tatctatcac actatggggt catggactgt gaccaatttt gtaccatcct
    12061 caaatgttgc ttcaacttga attgtatgaa taatgtcggt tacacctggc aaaacatcat
    12121 cttttcctaa aacttttttc ccagaactca ttaaatcgga aactgattta ccatcacgcg
    12181 ctccttctaa aatatgatca gcaatgatcg caactgactc tggatgattt aattttaacc
    12241 cccttgcctt tctttttcta gcaagctcag caatcataaa aacatgtaat ttatcaagtt
    12301 ctcttggtgt gagattcatg cataattttc cattatgtcg tatttatgct taggttttta
    12361 acaacaagct tagatgttta tattgattat caaacaataa attttagatt atgaaaaaat
    12421 caaaacgaat aaacagaaac ttgtctttta taggtattat aatactgggt gttcttgtgt
    12481 atgtgtttgg aacatctgta tacgagggac tgaatacagt ttcgccatga aaagatgaat
    12541 ctcaaaaagt ttttagtgtt agcgaatgtg tttggagttg ggtttatact attagttctt
    12601 tttgtgcctt aatcacaata aggcattaac aaaaaatata atcctaagga gatgaataaa
    12661 cattctatgc tgattgtaaa ttctattctt ggtaatatac acaatgataa tctattggaa
    12721 aataaagtgg atgatgcgag acagcagggt aaattaagac atttactttt gtccagaatt
    12781 gaaatggaaa aatcacgctt gagaaaaaaa actgaagatg gatttgatat aggtctagta
    12841 cttgatcctg gtaaaaaatt acatcatggc gatgttattt cacaagactc ggagattatt
    12901 ctaattgatc agttaccaga aaaaatacta actgtcagat ttattgaaaa cgataaaagg
    12961 ttatttcttt tggtcggtca tattataggc aatagacata ggccaatttc cataaaaaat
    13021 gaaacaatct cttttccaat ccatgatgat tcagaaatgg aattgtttgg gaagttattt
    13081 catgatgtga ttaataaaat tgatttgtca atgcaggagg aaatatttat tccacatgaa
    13141 agtatggatg tacatgaaca ctgactttag cgatttaagc ttactacaac tttctgattc
    13201 ttttttccct acaggacttt atacaatgtc taatggtttg gagactgttt tttctgaaaa
    13261 aacaataaca agtattgatg atctttatga actgataaaa acaaacatcg aacaacaaat
    13321 tggacctgct gattgtgtgg cacttgggtg tgcatatgat tttaccagtt taaacaatgt
    13381 tgaaaaaatc attatgtgtg atacatcatt atttgcaatg aaacttatcg gagaattgag
    13441 agaagcatca tgcagatctg gaacacagat gatgaaatgc gttggttctt ttgttagtaa
    13501 taatacatta tctgaatatc ataatgcaat aaaagaatct cattcacctg gaactcatgc
    13561 ggtagtcaca ggtgtggttt ctaacatatt aggaataaaa aaacaaaagt cagtattaca
    13621 ttttctttat ggattttctg tgagcatgat aggagcagca ttgagattag gaatgataga
    13681 gcattttcaa agccaaaaaa ttttacatca attaaaacct gtaattacag aaacatgcaa
    13741 tgaatatatt acaaaatcat tagatgatat gtggcaattt gcacctgaat atgatattat
    13801 acaaattgaa catgaacaac aattttctaa gatgtttatt acataactgg ttttgttaaa
    13861 atgaataaaa gaataccacg aataggaatt ggaggtcctg taggatgcgg aaaaagtatg
    13921 cttatcgaaa gagttgtacc tattctggcc aagaaaaatt atcagatcag tattatttca
    13981 aatgatgtta tatcacagga agatgctgat agaatgcgtc aaaacttggc tactgatcaa
    14041 ggattactac ctgaaaattt agtgataggt cttgccactg gtggatgtcc tcatacagct
    14101 gtacgtgaag atccatcaat caatttatcc gtaattgagc aattggaaag tgaacacact
    14161 gatctggatt taatcataat tgagagtggg ggtgataaca ttacaacaac atttagtcct
    14221 gctttggctg attattttat ttacattatt gatgtttcag gaggtgacaa atatccaagg
    14281 aaaagaggtt tgggtataga aacggctgat ctattggtta taaataaaat tgatcttgca
    14341 gaattggtta atgcaaatct ggatgtgatg gaaagagatg caaaaattgt taggaagaat
    14401 aagccttatg cacttgttaa ctgtaaaact gatcaaggca tagatacggt agtacagcat
    14461 ataatagaag atgtgttgtt tgatcaacca ccaaaaaaaa tggtgactag tgtaaaatga
    14521 ctgatttaaa attttgtgaa tcagatgatg ttccagcaat attctcagcg tatgataaaa
    14581 aaatagagca actggatgta ggcaaatcag gtaaggttgg acttttgaga ttaaaacttg
    14641 aggaaaattc agataaaaat aaaactataa tcactgacca atattctcag gttccacttt
    14701 atacacaaaa agcactgtat tatgatgaaa aactgccaaa tatggctcat ttgtttatca
    14761 tgtcaccatc agg
//
LOCUS       DASCPJ010000118         4109 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001841076, whole genome shotgun sequence.
ACCESSION   DASCPJ010000118 DASCPJ010000000
VERSION     DASCPJ010000118.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001841076"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="QGF01_05720"
     CDS             complement(<1..233)
                     /locus_tag="QGF01_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HJN20414.1"
                     /translation="MKILTIIPARGGSKGIQLKNLSKINDKPLVAFSIEHSLESKLIN
                     RTIVSTDNEEIAKVSEKYGAEIPIFRSKELAGD"
     gene            complement(230..1081)
                     /locus_tag="QGF01_05725"
     CDS             complement(230..1081)
                     /locus_tag="QGF01_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20415.1"
                     /translation="MFKKLTNSYLINSLVDVHDLLFTHPVLKKNVELKDKYNNERIFI
                     LGSGSSILHYDLKVLNKEYVMTQNSFHMHNDIFDINPTIHCVVPYYQSEKEYSTWIEY
                     IGDMKEKMPSTTFIWGLNTKALIDNHHPELIKQSYYIRTKYDSLTLNHATVDIAKTLM
                     NIPTVLTQCLSVAIYMGFREIYLLGFDLDQICHTNDRTFGRFYGMSKLTDSKFEEDAE
                     RNLKVETTDGWYNWWLMNKQFFLLKSYADQNKIKIINGTQGGILSYFKREPIENIIGQ
                     EILSEKK"
     gene            complement(1082..1783)
                     /locus_tag="QGF01_05730"
     CDS             complement(1082..1783)
                     /locus_tag="QGF01_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019846.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJN20416.1"
                     /translation="MKKIIKKIINLFMPIELFEHLRFDIRSKLGRLLSRSLKLNSENA
                     NFINLGSGNSYVNNFINIDFFGNKMIDYGMDLRYPFKIESNSIDGIFSEHTFEHLSHL
                     EVDNTLSECCRILKPEAKIRIIVPDLSILIERYCANDDEWFHNWHDLVLKDPSRHYMK
                     KYFTKMFAINFTASYYSHKSCWDFESLEKALSTHGFISIRKCDYNVGTPELLIDSDSE
                     DRKLVSIYIEGKKGS"
     gene            complement(1818..3134)
                     /locus_tag="QGF01_05735"
     CDS             complement(1818..3134)
                     /locus_tag="QGF01_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024832.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HJN20417.1"
                     /translation="MMNILKKAFQLKKHSGFMKYFANISWLMAERILRMIVTLFVGVY
                     VARYLGPERFGLLSYANSFVGLFLALATLGIDSIVIRELVKSPGSRNELLGSAFGLKI
                     LGTLLMWFIILFSVPLTENDTQTNIFIGIFSLAIFFQAFNVIDFNYQAEVKSKYVVQV
                     QFVQLFITSFTKLFLIFLKAPLLWFVWVYCFDAFLLALGLVGMYLLKSGNFWQWRWKW
                     KVARSLLRDSWPLIISGVLVSIYVNIDQVMIKHMIDEQAVGLYAIATRLSTAWYFIPI
                     AITSSMFPAILNAKYNNSSIYHQRLQALYDILVWMAILIAIAILCLSDSIVDLLVGHE
                     YAPAASVLSIAIIAGIFTNIGLINNKFFEAENRQIDILYRSLMGVSVNIILNIILIKK
                     YGIGGAALATVAAQFTTSIVYTYLKKDSRVLFFMFLKSFDARRIFS"
     gene            complement(3451..>4109)
                     /locus_tag="QGF01_05740"
     CDS             complement(3451..>4109)
                     /locus_tag="QGF01_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HJN20418.1"
                     /translation="AVEHLVYASSSSVYGANTNMPFSVHDNVDHPLSLYAASKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWDRPDMALQKFTRAILSGKKIQVYNYGKHRRDFT
                     YIDDIVEGVVRVLDKPAASNPEWNGSHPDSGSSSAPWLVFNIGNNNPVDLMDYIEALE
                     NALGKKAEKIFLPLQPGDVPDTYADVNDLEEQFNYKPSTTVDEGIALFIRWYLEYYES
                     "
BASE COUNT         1364 a          755 c          614 g         1376 t
ORIGIN      
        1 tcatctcctg ctagctcttt acttctaaaa atgggtattt cagcaccata tttttctgaa
       61 acttttgcaa tttcttcatt atccgtagag acaattgttc tgttaattaa ttttgattct
      121 aaagaatgct caatggaaaa tgctaccaat ggcttatcat ttatttttga taagtttttt
      181 agctggatcc cttttgaacc acctcttgca ggaataatag taagtatctt catttttttt
      241 ctgataatat ttcttggcct attatatttt caataggttc tcgtttaaaa taacttaata
      301 ttccaccctg tgtcccattg ataattttta ttttattttg atccgcataa cttttcagca
      361 gaaagaactg cttattcatt aaccaccaat tataccaacc atctgttgtt tcaactttca
      421 agtttctttc ggcatcttct tcaaatttag aatcggttag ttttgacatt ccataaaatc
      481 ttccaaaagt tcggtcattt gtatggcaaa tttgatctag gtcaaatcct aaaagataaa
      541 tttctcgaaa tcccatatat atagcaactg ataaacattg tgttaaaact gtaggaatat
      601 tcattaaagt ttttgcgata tccactgtgg catggttaag agtaagcgag tcatacttag
      661 ttcttatata gtaagattgt ttaattaact caggatgatg attatctatt agagcttttg
      721 tgtttaaccc ccatataaaa gtcgtactgg gcattttttc tttcatatcc ccaatgtatt
      781 cgatccaagt actatactcc ttttctgatt gataataggg aactacacaa tgaatagttg
      841 gatttatatc aaagatgtca ttatgcatat gaaagctatt ttgagtcatc acatattcct
      901 tattaagaac ttttaaatca taatgcaaaa ttgaactgcc actacctaat ataaatatcc
      961 tttcgttatt atatttatct ttcagttcaa catttttctt caaaacagga tgtgtaaata
     1021 acaaatcatg cacatctact aatgaattaa ttaaataaga atttgtaagt tttttaaaca
     1081 tttaagatcc ttttttacct tcgatataaa ttgaaactaa ttttctatcc tcactatcac
     1141 tatcaatcaa caactctggg gttccaacat tataatcaca ttttcttatg ctaataaaac
     1201 catgagtaga tagggccttt tctaatgatt caaaatccca acaggactta tgggaataat
     1261 aacttgcagt aaaattgata gcaaacattt ttgtaaaata ctttttcata taatgtctgg
     1321 aaggatcttt caaaaccaga tcatgccaat tatgaaacca ctcatcatca ttagcacagt
     1381 atcgttcaat gaggattgat aaatcaggaa caattatccg tattttagct tctggtttta
     1441 aaattcgaca acactcacta agtgtgttat ctacttcaag gtgagaaaga tgctcaaaag
     1501 tatgctctga aaaaatacca tctatagaat ttgactctat tttaaacgga tatctcaaat
     1561 ccataccata atcaatcatt ttgtttccaa aaaaatctat gttaataaag ttatttacat
     1621 aactgttacc actacctaaa tttataaaat tagcattttc actattcaat tttaaactgc
     1681 gagataataa ccgacctaac ttagatctta tatcaaacct taagtgttca aataactcaa
     1741 ttggcataaa aaggtttatt atttttttga ttatcttttt cattatctaa ttctataaat
     1801 gtaatattta aatatttcta tgaaaaaatc cttctggcat caaagctttt tagaaacata
     1861 aaaaacaaaa cccttgaatc cttttttaag tatgtataaa caatagaggt cgtaaattgt
     1921 gcagcgactg ttgccaatgc cgccccacca ataccatact ttttaattaa aataatattt
     1981 agaataatat taactgaaac acccatcaag gatctgtata agatgtctat ctgcctgttt
     2041 tctgcttcaa aaaatttatt gtttatcagt cctatgttgg tgaatatacc ggcaataatt
     2101 gctatactta gtactgaagc ggcaggcgca tattcatggc caaccaatag atcaacgatg
     2161 gaatcactta aacataatat agctattgct atcaaaattg ccatccacac taaaatatca
     2221 tataaagcct gtaatctttg gtgataaata gatgaattat tatattttgc attcaaaata
     2281 gctgggaaca tggaactagt tatggctatt ggaataaaat accaagccgt acttaaccgt
     2341 gtggctattg cataaagccc tacagcttgc tcatcaatca tatgtttaat catcacttga
     2401 tctatattta cgtaaataga aacaagtact cctgatataa tcaatggcca actatctcgc
     2461 agcaacgaac gtgcaacttt ccacttccac ctccactgcc aaaaatttcc acttttaagc
     2521 aaatacatac ctactaaacc caatgctagt aaaaatgcat caaaacaata aacccagaca
     2581 aaccaaagca ggggagcttt aagaaatatt aaaaacaact ttgtaaatga agttataaaa
     2641 agttgaacaa attggacttg aaccacatac ttagacttta cctctgcttg ataattaaaa
     2701 tcaataacgt tgaaagcctg gaaaaaaatg gctaaagaaa aaatgccaat gaaaatatta
     2761 gtttgagtgt cattttcagt taaaggaact gaaaataata taataaacca catgagtaat
     2821 gtgcctaaaa tcttaaggcc aaaagcgctt cctaacagtt cgttcctgga tcctggactt
     2881 ttaaccaact cacgtattac tatgctatct attcctaaag tagctagcgc aagaaataaa
     2941 cctacaaaac tgtttgcgta actcaacaat ccgaatcgtt cgggccccaa atagcgtgct
     3001 acataaacac cgacaaagag ggttactatc atccgcaaaa tacgttcagc cattagccac
     3061 gatatgttgg caaaatattt cataaatcca gaatgttttt ttagttgaaa agcttttttc
     3121 aggatattca tcatatgtga ctaactattc agtaatatgc ttcaattaat tcttataagg
     3181 aattttacac aactaaaccg aaatcttaat cagttgttct ttcgaaattc atctattcct
     3241 gtaactagtt acctcaagac tctggacttg gaatttttgt ccttgctcta taattataac
     3301 aaattgacta gtggagatta agctatcatg gatcgtgtat acaatcacac taactattat
     3361 acaaattctc ccaaataaag ttttccggat gtgtgattca tgttcttgac cgtaaagcac
     3421 tcaggtgagt acgaaaatat ttactttgct tcaagattcg taatattcaa ggtaccatct
     3481 gatgaatagg gcaattcctt catccacagt tgttgagggt ttgtagttga attgttcttc
     3541 caagtcattg acatcagcat atgtatccgg gacatctcct ggttgtagag gcaggaatat
     3601 tttttcggct tttttgccca aggcattttc aagtgcttcg atataatcca tcagatcgac
     3661 agggttgttg tttccgatat tgaagaccag ccatggagca ctacttgagc ctgaatcagg
     3721 atggcttcca ttccattctg gatttgatgc tgcaggcttg tctaaaactc ggactacccc
     3781 ttcgacgatg tcatcaatgt atgtgaaatc acggcggtgc ttgccgtagt tataaacttg
     3841 tatcttcttt cctgaaagaa tggctcttgt gaatttttgt agagccatgt caggacggtc
     3901 ccaaggaccg taaacagtga aaaaacgtag cccggtagta ggcaagccat agagatgact
     3961 gtatgtatgc gccattaatt cgttggcttt tttgcttgca gcatagagtg acagggggtg
     4021 atcaacattg tcatgtactg aaaatggcat gttggtattt gcgccataaa cagaactgga
     4081 agaagcatat accagatgtt cgacagcgt
//
LOCUS       DASCPJ010000119         3979 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001877943, whole genome shotgun sequence.
ACCESSION   DASCPJ010000119 DASCPJ010000000
VERSION     DASCPJ010000119.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001877943"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(129..1076)
                     /gene="thiL"
                     /locus_tag="QGF01_05745"
     CDS             complement(129..1076)
                     /gene="thiL"
                     /locus_tag="QGF01_05745"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HJN20419.1"
                     /translation="MSRLTEKKIIDIFQSTLNDKFVSEDVEYFKSGKKYFVLKIDTLV
                     ESTDMPKNVGLQYIARKSIISCISDFAAKGVKPIFGIVSVVLPRKLSKKKIEDLALGF
                     GKASKEFGIKILGGDTNEGKEISISFTLFGITNKIIKRKGAKLNDIIISSGPFGYSSA
                     GLSILQRKKKYSQSFGIKAKNAVINPNSRLMFGLKNKNYFSSSMDSSDGLSTTLEELA
                     RQSKRKFIITNVPAKADIFDFAAVNRLSAMNLIFNGGEEYEIIATVNPSNLTKIKKYA
                     KIHHIQLYEIGYVTKGNGVYLQKNGRFMKIKDKGWHHFR"
     gene            complement(1060..2358)
                     /locus_tag="QGF01_05750"
     CDS             complement(1060..2358)
                     /locus_tag="QGF01_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HJN20420.1"
                     /translation="MKISVSGIRGIFGKDLTLPDILKFSNNFSALIKSKKCVVATDTR
                     PSGNVILKTVKDCLIESGIDVYDLGIAPTPIAFREARKYGAGIEITSSHNPIEWNGLK
                     MIIDGKGLNEEQLHLVVGKQIHNKSKIGSEYFIKSNYINDVKKIIGKISGVPKITIDV
                     GGGAAKTIVAKLLEEIGCDVITINDDLEKSSRGPDPTTNPLKDLISSTNGRDVGFAFD
                     LDADRLVVVLNGKKMNPDVTLGLGVIKSLELGYKNFVFSQDTSISIEKYIKQKGGKVF
                     RSKVGESNVSMKMLETKSQVGGEGSSGGFIFSDFNHCRDGILTSGLISSMILKDTFHD
                     MLNFMEKYHIIRDKIECKLEHQDQVMKTIYKNLNERYNEIQTIDGIKVIVDENTWILI
                     RKSNTEDIIRMSIESESLEKSRNIHQETKELIKQSYEQIN"
     gene            2409..2482
                     /locus_tag="QGF01_05755"
     tRNA            2409..2482
                     /locus_tag="QGF01_05755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2443..2445,aa:Thr,seq:cgt)
     gene            complement(2700..2870)
                     /locus_tag="QGF01_05760"
     CDS             complement(2700..2870)
                     /locus_tag="QGF01_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20421.1"
                     /translation="MSKALEIIGLILIGGTIYSFLGMGESELRWPVFWGCAGGALTII
                     VYRNYMRKRKRK"
     gene            2922..3137
                     /locus_tag="QGF01_05765"
     CDS             2922..3137
                     /locus_tag="QGF01_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20422.1"
                     /translation="MDKYLLVVMGILMIGIPIAFVSPTGEIRDQPFIPLFYVSIGGII
                     VIIVYSSYKQKKETQRANRERRRKSKK"
     gene            complement(3140..3400)
                     /locus_tag="QGF01_05770"
     CDS             complement(3140..3400)
                     /locus_tag="QGF01_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJN20423.1"
                     /translation="MQIMADLLTATEESGQQGIKTTSLLLKANLSHSRLERFVSNLTG
                     AGLVNKIEYDGRNVFVITSKGKQYLEQYKKFADVAESFGLEM"
     gene            3507..>3979
                     /locus_tag="QGF01_05775"
     CDS             3507..>3979
                     /locus_tag="QGF01_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20424.1"
                     /translation="MSSEYKVNWFNDFLGVAYRYYDLRMNVVPLFSDFKDASKFWRET
                     VHWWIDHSIKIRFVEIENDYWFIMGSDSKRVENNSSLFKILPKSDYYERFKKGHDGEA
                     YLRLGVYTKKSKKDVKDDAVCSCSHVKEDHGEEDDDSCLYEDCNCKKFESFQVNLL"
BASE COUNT         1230 a          694 c          565 g         1490 t
ORIGIN      
        1 ggtttctgtg acgggagaat ccacattttc ctctttttca acaacagctt ctgtttcaga
       61 caatgcatac agacacttga aggggtttaa aaaacattca caaatacaat tttttctata
      121 ttagccagtt atcgaaaatg atgccatccc ttatctttta ttttcataaa cctgccattt
      181 ttttgtaaat acaccccatt accttttgtc acatatccaa tttcataaag ttgaatgtgg
      241 tgaatttttg catatttttt aatttttgtt aggttagagg gatttacagt cgcaataatc
      301 tcatattctt ctcctccatt aaagatcagg ttcatagcac tcagtctatt aacagcagca
      361 aaatcaaaaa tgtctgcttt tgcgggaacg tttgtgataa taaatttccg tttactttgt
      421 cttgccaact cctctaaagt tgtagataat ccatcacttg aatccataga tgatgaaaaa
      481 taatttttat tttttaatcc aaacattaat ctagaatttg ggtttattac tgcatttttt
      541 gcttttattc caaaactttg agaatatttt ttctttcttt gtagaatgga aagacctgca
      601 gaagagtatc caaacggtcc agaactaatt ataatatcat tcagtttggc tccttttctt
      661 tttatgattt tatttgtgat gccaaaaagt gtaaaactaa tcgagatttc tttgccttca
      721 ttggtatctc cacctagaat ttttatgcca aattcttttg atgctttccc aaaaccaaga
      781 gctaaatctt ctatcttctt ttttgaaagt tttcttggta aaactactga aacaatacca
      841 aaaattggtt tgactccttt tgcagcaaaa tcacttatac aagaaataat actttttctt
      901 gcaatatatt gtagtcctac attctttggc atgtcggtac tttctactaa tgtgtctatt
      961 tttaaaacaa aatatttttt cccgcttttg aaatattcta catcttctga tacaaattta
     1021 tcatttaacg tactctgaaa aatatcaatt attttttttt cagttaatct gctcataact
     1081 ttgtttaatt aattctttag tttcttgatg aatgtttcta cttttttcta gactttccga
     1141 ctctattgac attctgataa tatcttcggt gttagatttt cttattaaga tccaagtatt
     1201 ttcatcaaca atcaccttaa ttccatcaat tgtttgtatt tcattatatc tttcattcag
     1261 gtttttgtaa atagttttca taacttgatc ttgatgttct agtttacact ctatcttatc
     1321 tctgattata tgatattttt ccatgaagtt taacatatca tgaaatgtat ctttcaatat
     1381 catagatgaa ataagaccac tcgttagaat tccatcacgg caatgattaa aatcagaaaa
     1441 tatgaaacca ccactactac cctcgcctcc tacttgagat ttagtttcaa gcatcttcat
     1501 actaacgttg gattctccaa ctttggagcg gaaaacttta ccgcctttct gttttatgta
     1561 cttctcaatt gatatgcttg tatcctgact gaatacaaaa tttttataac ctaattctag
     1621 tgatttgatt acgcccagtc ctaatgtaac atcaggattc atttttttac catttagtac
     1681 aactacaagt ctatcagcat ctaaatcaaa agcaaatcct acatctctac cgttagtact
     1741 tgatattaga tcttttaatg ggtttgttgt aggatctggt cctctggaac ttttctctag
     1801 atcatcattt attgtaatca catcacaacc aatttcttct aataattttg caacaatagt
     1861 ttttgctgcc ccaccgccaa catcaatagt tatcttagga acaccagata tttttcctat
     1921 gatttttttt acatcattga tgtaattgga tttgataaag tattcagaac caatttttga
     1981 tttattgtga atttgtttac ctacaactag gtgtaactgt tcctcattga gacccttacc
     2041 atcaataatc atttttaatc cattccattc gattgggttg tgtgatgaag taatttctat
     2101 gcctgcacca tatttccttg cttcacgaaa tgctatagga gttggtgcta ttcccaaatc
     2161 ataaacatct ataccagatt ctatgagaca atctttaacg gtttttagta taacattacc
     2221 tgatggtcta gtatcagttg caacaacaca tttctttgat tttattaacg cagagaaatt
     2281 atttgagaat ttcaaaatat caggtaatgt taaatcttta ccaaaaatac ctctaattcc
     2341 agaaactgat attttcaatg tttaaactta gataggcttt ttataaactc tgagtgcaaa
     2401 tgattagcgc ctcgatagct cagcctggta gagcgaacgc ctcgtaagcg ttaggtcgcg
     2461 agatcgaatc tcgctcgagg ctttttaata ttttaaaaca gtttatgcta tgataaatta
     2521 attctagata acggatgatt ttctaaatca gttttcaaag gtttcaatat cttcttcaag
     2581 atcttttcca agatctttcc accattctgt agtttcttta gacattgctc ccatcgatta
     2641 ccttgtttag tcctttatag atctgccggt tcgtccatta attattgctg atcgtaattt
     2701 tattttcttt ttctttttct catatagttt ctgtatacta ttattgttaa tgcaccacct
     2761 gcacagcccc aaaagactgg ccaacgaagt tccgattcac ccattccaag gaatgagtaa
     2821 attgttccac cgattaaaat caaaccaata atttcaagag cttttgacaa ttcatattat
     2881 aataattaaa aagatatatt gttttacaat tacagatatc catggataaa tatctactag
     2941 tggttatggg tatattgatg attgggatac cgatagcatt tgtatcaccc acaggagaga
     3001 taagggatca accatttatt ccattatttt atgtctctat tggcggtatc attgtaatta
     3061 ttgtttacag ttcttataaa caaaagaaag aaacacaaag agctaataga gaaagaagac
     3121 gaaaatcaaa aaagtagttt tacatttcta atccaaaaga ttctgcaaca tctgcaaatt
     3181 ttttgtattg ttcaagatat tgcttacctt tagaagtaat tacaaataca ttccttccat
     3241 catactcgat tttattgacc aaaccagctc cagtcaagtt gcttacaaat ctctctaatc
     3301 ttgaatggga aagattagcc tttaaaagta atgatgtagt tttgatccct tgttgtccag
     3361 attcttctgt agctgtcaga agatcagcca taatttgtat gctagttcga taaacaacca
     3421 ttatccttaa tcacatcatc tctgatataa gaatataact gtaaaattgg atcaaataca
     3481 tataatctta attttactct ttaacaatgt cctctgagta taaagtcaat tggttcaatg
     3541 atttccttgg cgttgcttat cgatattatg atttacgaat gaatgtagtt ccactatttt
     3601 ctgattttaa ggatgcgtca aaattttggc gtgaaactgt tcattggtgg atcgatcatt
     3661 ctattaaaat tcgctttgtt gaaatagaaa atgattactg gttcataatg ggatcagact
     3721 ctaaacgagt tgaaaataat tcatctctct tcaaaatttt gccaaaatct gattattatg
     3781 agagatttaa aaaaggtcat gatggggaag catatcttcg attgggtgta tatacaaaga
     3841 aatctaaaaa agatgtaaaa gatgatgctg tctgtagttg tagccatgtg aaggaggatc
     3901 acggagaaga agatgatgat agttgcttat atgaggattg taattgtaaa aaatttgaaa
     3961 gtttccaagt aaacttgct
//
LOCUS       DASCPJ010000120         3938 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001891834, whole genome shotgun sequence.
ACCESSION   DASCPJ010000120 DASCPJ010000000
VERSION     DASCPJ010000120.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001891834"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="QGF01_05780"
     CDS             <1..585
                     /locus_tag="QGF01_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HJN20425.1"
                     /translation="ILVQLPLPKHLDEFATVSRILPIKDVDCLTPQNVGLLAIGKSIL
                     KPCTPSGIMEMFYYYKIKLEGKNVVLINRSNLVGKPLYHLLLQNNATVTTCHSKTKNL
                     KEICQSADIVITGVGDRTKFTLTSDMIKKDSIVIDVAISSQDGKLVGDADFDDVIQKA
                     SFVTPVPGGVGPMTVAMLLKNTVTAASLNKEIVS"
     gene            575..1135
                     /locus_tag="QGF01_05785"
     CDS             575..1135
                     /locus_tag="QGF01_05785"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HJN20426.1"
                     /translation="MSVDIEKAALRKHFLEKRDAVSADLREISSIKIYNHLKQSDLYK
                     HSQNISCYFPIGSEVNTHSIMSDILANGKNLLLPTVVNNDLEFRIVTDIKKLEKGHFD
                     IMEPRDDCKKSEEIDCVIVPTVAVSKSGTRLGYGSGYYDKFLSRTNAIKITLTYSTQI
                     VGSIPSDSHDVNMDWIVTEEGIIKSS"
     gene            complement(1130..2392)
                     /gene="purD"
                     /locus_tag="QGF01_05790"
     CDS             complement(1130..2392)
                     /gene="purD"
                     /locus_tag="QGF01_05790"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HJN20427.1"
                     /translation="MTNVLVVGSGAREHSLGWKISQSEHVDDVFYAPGNGGTKNNIPI
                     NVEQIEELVDFASKNDCFTVVGPEVPLALGIVDTFMEKNLKIFGPTQNAAKLESSKIW
                     AKNFMDRNEIPTAKFKIFNEPDLAKEYIESVSFPLVIKADGLAAGKGVIVCDTTDEAL
                     CAVDKILVKKSFGEAGKRIVVEERIDGIEASYIALSDGETVIPLATSQDHKRIFDDDK
                     GSNTGGMGAYSPTGAIDDALAEKIQTKIIRKTVDSLKKEGIVFKGFLYAGVMIRDGEP
                     YVLEFNARMGDPECQPMVIRMNSDLFEYLTAVTDGNLSSLPSMSWKKQSAVCVVLASN
                     GYPESYLKNEEITGLSDIPKDSFVFHAGTKDVSGKIVTNGGRVLSVTSLGDSLQDAIS
                     NTYKAAEKISWPSKYYRHDIGKKGLSYL"
     gene            2564..3673
                     /locus_tag="QGF01_05795"
     CDS             2564..3673
                     /locus_tag="QGF01_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HJN20428.1"
                     /translation="MGIPEKIKAIQDEMAKTQINKATEHHIGLLKAKIAKLKREQEFT
                     AKKESASKKDGFSVRRTGDATVVFIGLPSVGKSTLLNKLTGSHSSIGAFQFTTLTVVP
                     GVMKHRGAVIQLLDLPGIIKGASSGKGLGKRVLSVARSADLVLLILDVFNPYHEKVLI
                     TELDNIGIRLNQKPPNIVIEKSSSGGISIAKQVKMTKISEKLVKQILNVYGIVSAGVV
                     IREDISSEQLVDFLIGNRTYTQSLAVLNKIDLVNRSFLNKFSKKVKTEYVSVSAESET
                     NIELLKEKIYEKLNFIRIYMRPKGGAIDYKEPLIITNSSKIGDVCNKLHRKMKKEFRY
                     ALVWGKSVKYDGQRVGIDHILQDEDVLTIIKRKGI"
BASE COUNT         1429 a          702 c          689 g         1118 t
ORIGIN      
        1 attctcgttc agctaccact acctaaacac cttgacgaat ttgcaactgt ttctagaata
       61 ttaccaataa aggatgttga ttgtttaaca cctcaaaatg taggtcttct ggcaatagga
      121 aaatccatcc taaaaccttg cacaccatct ggaataatgg agatgtttta ttattacaaa
      181 attaagcttg aaggaaaaaa tgtagttttg attaatcgta gcaacttggt aggaaagcca
      241 ctctatcact tgttattaca aaacaatgcc accgtaacaa catgtcactc aaaaactaaa
      301 aacctaaaag aaatttgcca atccgcagac attgttatta ctggtgtagg tgatcgaaca
      361 aagtttacac taacttcaga tatgattaag aaggactcaa ttgtgataga cgttgcaata
      421 tcaagtcaag atggaaaatt ggttggtgac gcagattttg atgatgttat acaaaaggca
      481 tcatttgtta caccagttcc tggtggagta ggaccaatga cggttgcaat gctattaaaa
      541 aacacagtta ctgccgcatc attaaacaaa gaaattgtca gttgatatag aaaaggctgc
      601 attacgcaaa cattttcttg agaaacgaga tgccgtatct gcagatttaa gagaaatttc
      661 tagcataaag atctataatc atcttaagca aagtgatctc tacaaacatt cacagaacat
      721 atcttgctat tttcctattg gaagtgaggt taatacacat tcaatcatgt cggacatatt
      781 ggctaatggg aaaaacctgc tgcttcccac agttgtaaac aatgatttag aattccgtat
      841 agttacagac atcaaaaaat tagaaaaagg acattttgac ataatggagc caagagacga
      901 ttgtaaaaaa tccgaagaaa tagactgtgt aatagtccca acagtagcag tttcaaaatc
      961 aggaacaaga ttagggtacg gaagtggata ttatgacaag tttctctcta gaacaaatgc
     1021 aataaaaata acattgacat actccacgca gattgtagga tcaattccaa gtgatagtca
     1081 tgatgtcaat atggattgga tagtaacaga agaagggatt atcaaatcat catagatacg
     1141 acagaccttt tttaccaatg tcatgtctgt aatatttact gggccaagaa attttttcag
     1201 cagctttgta tgtatttgaa attgcatcct gtaatgaatc acccaaagac gttacactca
     1261 gtactcttcc accattggtc acaattttac ctgaaacatc tttcgttccg gcatgaaaca
     1321 cgaatgagtc ttttgggata tcagacagac cagtaatctc ctcatttttt agatatgatt
     1381 ctgggtaacc atttgaagct aatacaacac aaacagcact ttgttttttc catgacatag
     1441 aaggcaaaga cgaaagattt ccatctgtca ctgcagttaa atattcaaac aaatctgaat
     1501 tcattcgtat taccattggc tgacattccg ggtcacccat acgtgcatta aactctaaaa
     1561 cataaggttc cccatctcga atcattacac cagcgtacaa gaaacccttg aatacgattc
     1621 cttctttttt taacgagtca acagttttac gtataatttt tgtttgaatt ttttctgcca
     1681 atgcatcatc tattgcaccg gttggagaat atgctcccat accaccagtg ttagaacctt
     1741 tgtcatcatc aaagattcgt ttgtgatcct ggcttgtagc taacggtata acagtttcac
     1801 catcagacaa tgcaatgtat gacgcctcaa ttccatcaat acgctcttct acaacaattc
     1861 tttttcctgc ttcaccaaat gattttttca ccaaaatctt atcaacagca cataacgctt
     1921 catccgtagt atcacataca atgacaccct tgccagctgc aagaccatca gcttttatga
     1981 ccagtggaaa tgacaccgat tcaatatact cttttgctaa atctggctca ttaaagatct
     2041 taaacttggc agttgggatt tcatttctat ccataaaatt ttttgcccat attttacttg
     2101 attctaattt tgcggcattt tgtgtaggac caaatatttt caaatttttc tccatgaacg
     2161 tgtcaacaat acccaaagcc agtggaactt caggaccaac taccgtaaaa cagtcatttt
     2221 tagacgcaaa atctactaat tcttcaattt gttcaacatt tattgggata ttatttttcg
     2281 taccaccatt tccaggagca taaaacacat catcaacatg ttcactctgt gaaatcttcc
     2341 aaccaagtga atgttctcta gcaccagaac caactactag tacattagtc aataatcgga
     2401 tatggcaacg caagtatata atccaaggtt tgaaagtcta tctcaaaatg aactatttgt
     2461 atccttgtca accaaatcat gtcaataaaa aatatgataa gtaacactga actaaaccga
     2521 tagactgtaa ttatgatgat tttgtattat cataggtaca ataatgggaa ttcctgaaaa
     2581 gataaaagct attcaagatg aaatggcgaa gacgcagatt aacaaagcga cagaacatca
     2641 tataggtctg ctcaaggcaa aaattgcaaa gttaaagcgt gagcaagagt ttactgcaaa
     2701 aaaagagtca gctagtaaaa aagatggttt tagtgtaaga agaactggag atgccactgt
     2761 agttttcatt ggactgccaa gcgtaggaaa atcaacattg ctaaacaagc ttacgggctc
     2821 gcactcttca attggtgcgt ttcaattcac cacactcaca gttgtaccag gtgtaatgaa
     2881 acatagaggc gctgtaatac agctgttaga tctaccggga attatcaaag gtgcttcatc
     2941 aggaaaaggt ttaggtaaac gagtattatc tgtagcaaga agcgcagatc tggttttatt
     3001 gattttagat gtctttaacc cataccatga aaaagtgttg attacagaac tagacaatat
     3061 tggaatcaga cttaaccaaa aaccccccaa tattgtaata gaaaaatcat catcaggtgg
     3121 aatatctatc gccaagcaag taaagatgac aaaaatatca gaaaaactgg taaaacaaat
     3181 tctcaatgtt tatgggatag ttagtgctgg agtggtgata agggaagata tatcatctga
     3241 acaactggtg gactttctaa ttggaaacag aacatataca caatcattag cagttttgaa
     3301 taaaatcgat ctggttaatc gtagtttctt aaacaaattt tcaaagaaag tgaaaacaga
     3361 atatgtttct gtatctgctg aatctgaaac aaatattgaa cttttaaaag aaaaaatata
     3421 tgaaaaactg aactttatta gaatatacat gagaccaaag ggcggtgcga tcgattacaa
     3481 agaaccattg ataattacaa atagtagtaa aattggagat gtttgcaata aattacatcg
     3541 aaaaatgaaa aaagaatttc gttatgcctt ggtatggggt aaaagtgtaa agtatgatgg
     3601 acaaagagtt ggaatagatc atattttaca agatgaagat gttttgacta tcataaaaag
     3661 aaaaggaata tgacaaaaca cttctaatta ttttctaaaa agtaaaaaaa accgttgata
     3721 aattgtaatc agtttgtata ttttttttag tataatgata gaaaagcgac aaaaaattgc
     3781 acaaaacaat ggcaactaaa agaaaagcac cagccaagag aaaaggcggt gcaaaaagaa
     3841 aagcacctgc caagagaaaa ggcggagcta agagaaaagg cggagctaag agaaaagccc
     3901 cagccaagag aaaaggcgga gccaaaaaag gcggagct
//
LOCUS       DASCPJ010000121        17402 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001904377, whole genome shotgun sequence.
ACCESSION   DASCPJ010000121 DASCPJ010000000
VERSION     DASCPJ010000121.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 17402)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17402)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17402
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001904377"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(13..1005)
                     /gene="ftsY"
                     /locus_tag="QGF01_05800"
     CDS             complement(13..1005)
                     /gene="ftsY"
                     /locus_tag="QGF01_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HJN20429.1"
                     /translation="MFDKLSNAFSNVAKSLGEKELKEKDIEDVISELEISLLESDVAT
                     EVIDGIKSNLTEKLIGSKVNKKEIEHFVKQSLIQNISNMFDEAGSVDILSNIKSKTNP
                     QEPYLILFIGINGTGKTTTIAKIANLLQKNKISVVVAASDTFRAGAIEQLREHTNKLN
                     LKLVAQNYGSDPAAVARDALLYAKSHKVDCVLIDSAGRMQTNKNLMEQITKISKVVNP
                     DLKIFVGDSLAGNDTVSQARTFNEHTDFDGAILTKSDADVRGGAAISIVAVTKTPVIY
                     IGTGQGYDDLKPFNKDTFLETIFGKPEPVAEPEPVAEPEPVALPSVLEMIYRKI"
     gene            complement(1016..1432)
                     /gene="pfdA"
                     /locus_tag="QGF01_05805"
     CDS             complement(1016..1432)
                     /gene="pfdA"
                     /locus_tag="QGF01_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HJN20430.1"
                     /translation="MSDEQTQQLLYRTQVLEGYFSELNQKESSIMSIIREATSAIECL
                     KSINDKDDNETLMPVGLGTFVNTKITPNKKLILNIGAGVAVEKDKNSAINFLESRLKE
                     MQIALQETAGQKQQVSEGLQQAKLEMEKLMTAHNQK"
     gene            complement(1512..2132)
                     /locus_tag="QGF01_05810"
     CDS             complement(1512..2132)
                     /locus_tag="QGF01_05810"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HJN20431.1"
                     /translation="MGKYTLPDMPYAYDALDPHIDARTMEIHHTKHHQKYTDGMNGAL
                     EKLSPELQSKDIVDILSNINDVPDDVRGAINFNGGGYDNHKLFWNNMKPNGGGEPGGV
                     LADAINASFGSFADFKEQFSSKTAPIQGSGWGWLVYNPSSSKVEYKAMSNQTSPRTEG
                     LIPLLGLDVWEHAYYLKYQNKRPDYIAAWWNVVNWDEVDNRLSKAK"
     gene            2203..4233
                     /locus_tag="QGF01_05815"
     CDS             2203..4233
                     /locus_tag="QGF01_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HJN20432.1"
                     /translation="MDVRKIFFLIVFVSIGMILLSSQLQNQDNISIQVNDSGSYIGTD
                     IPHSYGYDGTGIIISVIDTGVDFNHPDLLGFGSDGKVIGGYDFVDNDHIPEDVNGHGT
                     QVAGIIASNGILQGIAPNSKILAYKVSKDGESVPSNLIIKAIEKSIEDGADIINISLG
                     VNQTSAKIDQAVTKAVQNGIIVVTAAGNFGPELSTIGSPGINPNAITVGSTFNNVPSS
                     LVSTLKINDKEFNVFPMVGTQYIEESIVSEIVFGEYGRVKDLSDIDVQNSILLIERGS
                     DIENEIVFFSDKEKNASELDAKAIIVYNNEPGIFFGELIHEFVEDDYNPTIPALSLSR
                     EDGLFIKEMLKIHIEAELDVFYHPDFVAYFSSRGPVSPFYIKPDMVAPGAFISTTDLA
                     GNYSISSGTSFAAPHISGVSALLLQKNPELTSKELKSILMTTSDLVSDEYNKRFPLEV
                     SGSGRVNATKAINAELIITPANLIFNLSPYDQTETKIVKIKGMDDKSLSVKFENNQVI
                     DLKYYFEQNDLIITAKLIQHDLGEFEDRMIISHDNIDYQIPILLHVSEGAITINEDDG
                     ELDIVIVNPQSWSYAKVTAINKDTGKILTDSIVPNKTSEFVIHEPGEYWIEAKIESDE
                     NTLNVFDTIKIETVSEKNSINTLNFPEKPILIIAAIMIVTVIVGLSIRRTSN"
     gene            complement(4237..5826)
                     /gene="pckA"
                     /locus_tag="QGF01_05820"
     CDS             complement(4237..5826)
                     /gene="pckA"
                     /locus_tag="QGF01_05820"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HJN20433.1"
                     /translation="MSENMSEAFSSQIEKFGIKPSKIHRNYSAEKLVEMAVQKNEGIV
                     TSTGSLSVKTGNYTGRSPEDRFIIYDELTHNTVDWGKINKQFPSEKFEKILESMKKSV
                     ENKEVFIFDGFIGADPENRLSVRVINDHAWQNLFVHQLFIRPSKAELQSHEPEFTVMC
                     INDFDAIPEIHGTRSNAFILINLTKKIVLIGATNYAGEMKKAMFSVMNYFMPTKGVFP
                     MHCSANIGKDNDTVLFFGLSGTGKTSLSADPERMLIGDDEHGWSDKGIFNFEGGCYAK
                     CINLKEEEEPQIWNAIKSGAVLENVVIDKESLKPDFDDGTLTENTRAAYPLDFIPGSI
                     IPSVGGNPKVIVFLTADALGVLPPVSKLTKESAMFHFMSGYTSKLAGTERGIKEPRAT
                     FSVCFGAPFMPRLASVYAKMLGEKISKHNTVVYLINTGWSGGPYGVGKRINIKYSRAM
                     VKAALSGALDIVKYRHDEIFNLDIPTECPGVPAEVLIPKNTWIDKDSYTLSAKKLAQM
                     FIDNFKRFDDSDEIRKAGPKA"
     gene            5974..6378
                     /locus_tag="QGF01_05825"
     CDS             5974..6378
                     /locus_tag="QGF01_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HJN20434.1"
                     /translation="MPINKEKLALRDQETKSTPKFIRPESWRYKKLKTAWRKPKGVDN
                     HQRKQKSRGRPGLPKVGYRGPKSARGLHPSGFTDNLVYNINDLDSLNPKEDAVRIGHS
                     VGTKKRIVIVTKALEGKFKVLNARVNRDASKS"
     gene            6362..6817
                     /locus_tag="QGF01_05830"
     CDS             6362..6817
                     /locus_tag="QGF01_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HJN20435.1"
                     /translation="MPVNLSAKKRLVSRMTGVGIKRIKFDVDHLDDVADAITRQQIRS
                     LITANTIRIKQIVGTSRGRAQEKKNQKKKRGMTQGSKKGRKGARVGKKEVYVTKVRSL
                     RRRLKIAKERKEITNKNFWEIYTKIRGNTVRNIAHLRTLIEEIKTKGKD"
     gene            complement(6814..7113)
                     /locus_tag="QGF01_05835"
     CDS             complement(6814..7113)
                     /locus_tag="QGF01_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HJN20436.1"
                     /translation="MSISIKVYRKLLQVPKGKVTTYSDLAKAVGLKNGQRFVGTVMKK
                     NPYPVIIPCHRVVKSDGKIGGYSYGEKVKLKMLEKEGIKIKNGKIIDFDNEKFYF"
     gene            complement(7110..7886)
                     /locus_tag="QGF01_05840"
     CDS             complement(7110..7886)
                     /locus_tag="QGF01_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HJN20437.1"
                     /translation="MDLLVAYADDPAGGNMANFVSRDLVKDGDVFRGEYFDLAIIPTP
                     AISADWIEDKFDYDGYIFLSKHAAESGVLALTCHNTGNFSAANFGGYERQVSIPHSNL
                     QKSYIQNLWDARNQFTKFQITIEATHHGPTSLEKPTLFIEIGTTPKEWNDVNLCNSVA
                     QIVVQTMKQNQKSYPTGICFGGTHYSDKFTSELIHGKYALGTVIPKHALDEIDQFLFS
                     HIIERNRGATTALLDWTGMGKNKHKILEMLEGTDLEIIKL"
     gene            7927..8106
                     /locus_tag="QGF01_05845"
     CDS             7927..8106
                     /locus_tag="QGF01_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HJN20438.1"
                     /translation="MKFNARRTILNLKNTVKLTKKSDKDVYIQHLRLVLFGIAAVGTL
                     GFGIQFIFSVFTFGR"
     gene            8109..8867
                     /locus_tag="QGF01_05850"
     CDS             8109..8867
                     /locus_tag="QGF01_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HJN20439.1"
                     /translation="MSESEPIDEQKPEPMSEHDSLDELLAEKPASQKSDIEEKESQVE
                     EEAKVPAVEPESGDQTSHLFAVRTTGGQEKIVMRLLEAKMRMDQKDEEEVQKAKEEGK
                     EIKIKDKTLIDKDWKKAGDLENSLYVDEEFPRVKDVQTVLLLDDLKGYVVLEAQDPPS
                     MFNAIQGIRHIRGQLRGELQYSEIDKYLIKKSTVTELSVDNTVEIIGGPFKGMKATVK
                     RVDQEKEEAVVILLDAPYQLPVTVDANHLKKVLS"
     gene            8902..9384
                     /locus_tag="QGF01_05855"
     CDS             8902..9384
                     /locus_tag="QGF01_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HJN20440.1"
                     /translation="MGDKQTISSLVTGGEASAGPPLGPALGPIGVNIMEVINAINEKT
                     KEFQGMKIPVSVIVDTESKKWEIEVGIPSASALILKEAGIQKGSGTSGTEWVGEVSAD
                     MITKIANVKLESSYASSLKSVAKQIVGTCQTLGIKIEGKTPKEFTSEINEGKWNSKFE
                     "
     gene            complement(9436..9903)
                     /locus_tag="QGF01_05860"
     CDS             complement(9436..9903)
                     /locus_tag="QGF01_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJN20441.1"
                     /translation="MHGLDELDLKILFELTKDGHITVPLLSKKLGSNASVLYSRIKRL
                     ERRKLIKKFTIDIDDSLLGINVKATVGINRDPKLKVEIHKKLMGVPAIISISEVTGRF
                     DIMMQVHTKDLEELHMVVIDKIGKIDGIQNTETFVELQKTDKDPFYLINDTKI"
     gene            10004..10657
                     /locus_tag="QGF01_05865"
     CDS             10004..10657
                     /locus_tag="QGF01_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HJN20442.1"
                     /translation="MVTESQLVELIKQAKENGKERKFKQSLEMIMVFKDVDVKKGFAI
                     NETIQLPQKMGDAAQVCIIASGDMGVKAKNANADRIINEDELTKLGANKRESRKVINK
                     YDFFLADTKLMPTVGKVLGQLLGPRGKMPTPVPFNAPIESFLERFKSSVRIRVKNSLS
                     LSCKIGEEDMDEQHVAANANTITNAVEKKLPNGDKNIKKIMIKTTMGKAVKLEQVKK"
     gene            10660..11526
                     /locus_tag="QGF01_05870"
     CDS             10660..11526
                     /locus_tag="QGF01_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HJN20443.1"
                     /translation="MHENRTKFPKKKMRMYQMMEELPKKYSVTALVRMEKVRASQLLP
                     LRKKLKGEVEIISIKDKIAQKAFEKLDISGIKKLKENLTGQCLFMFTNMSPFKLNVLL
                     GKNKIMLAARGGDIASMDVVVPPKNTGIAPGPMLTEFKENKIPTKIDQGTIWILKETT
                     PIKKGEVISTKLAALLGKLDIKPIEAGIVLNSALSEGVLFTENELVVDVDLFRKQISE
                     AHQNAVSLSIEAAYITNENIEQILAKASQSARSVSLEIGYMTDDNKEQVIQKADGHAK
                     SVVSKTKGYTPT"
     gene            complement(11523..12164)
                     /locus_tag="QGF01_05875"
     CDS             complement(11523..12164)
                     /locus_tag="QGF01_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HJN20444.1"
                     /translation="MVDLSTTPELFIYILDLFGTMAFAVTGAFKAIEHKADIVGIIIL
                     ATITGVAGGTIRDIILGKTLPNSLIDPVYVIITIISAIVLFFLYSKLRQHWNIFLKFD
                     ALGLGIFTVIGATFAYNLVGMNFLVIVLSGMLTAIGGGILRDIFVNQTPIVFVKELYA
                     SASFIGAVLFYLTLLITNEIYAATIIGMVITTVLRLVAMKYNWNLPRVKSRYD"
     gene            complement(12229..12540)
                     /gene="rpl12p"
                     /locus_tag="QGF01_05880"
     CDS             complement(12229..12540)
                     /gene="rpl12p"
                     /locus_tag="QGF01_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HJN20445.1"
                     /translation="MEYVYAALMLHKLKKDVTEENITSIVKASGVEVNEAQVKSLVAS
                     LADVDIEEAIKAAPVATAAAPAAAPAEGGEGKKEDKKQDEAASAKSEEKAMEGLSSLF
                     G"
     gene            complement(12599..13111)
                     /locus_tag="QGF01_05885"
     CDS             complement(12599..13111)
                     /locus_tag="QGF01_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20446.1"
                     /translation="MLGRKKKGLKEGAFVFATQSNGKYNDFIVGAVTGINGKKIGING
                     VIVDPIGLKNKVEQGKAGQRSIEILKNPNPDNCIHALIYRIEHENYTGVIDLNESQLL
                     EIPPNVFLALDGWVRESFSELLNNVLSLPPGSERDEAKRIVKQRMDTLFDKNLKRTLY
                     AICRSLKILN"
     gene            13189..15882
                     /gene="alaS"
                     /locus_tag="QGF01_05890"
     CDS             13189..15882
                     /gene="alaS"
                     /locus_tag="QGF01_05890"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HJN20447.1"
                     /translation="MDKKEILSKFSTDPDRYYKVKLFEDVGFERKSCKTCKRFYWTLD
                     ENRINCPDHSSDTYSFIGNPPTNKRFDYTQAWKEVESFFVKNNHTSVNRYPVVCRWRD
                     DLYFTIASIVDFQRVMGSKVIFEFPANPLVVPQTCLRFKDLENVGVTGRHFSSFCMIG
                     QHSVPDNNGYWKDECINLDFDLLTKQFGIDKNEIVFVEDVWEGGGSFGSSLEYFVRGL
                     ELGNAVFTEFQGDLSNYKTLGQRIIDMGAGLERFAWITMGTPTAYDCCFGPITKNLIQ
                     QVGIETDSNILVPYFTEIAKNFEMYEDLTLVRKNAIKNSGLSDEQIRKIVTPLEGIYL
                     IIDHVRTLIFAISDGALPSNVGGGYNLRIMLRRIISTMNRLSLQFDINEIIDTQIDYL
                     KNTYPELEKTRNDVKEIIRIESERYGISKQRMQKIATNLNKDPTVDDLIRLYESDGVT
                     PDYLKEIKVISEIPSTFYSKLSELHQGKKQKEHLNLPLENISDTELLFYSNDPKEFDA
                     KVLKSFDNYVVLDRTSFYARGGGQEPDHGKIDNYEVVDVTKHGDIIIHELKGGIPKQG
                     ETVSCIINSTRRDRITKNHTSTHVINTSARSVLGSWVWQHSAFKDEDHARLDITHHSA
                     LTDEEVLQIEQAANSIIEKNIPVKIENFDRGTAEQKYGFRIYQGGVVPVKSVRIVSIG
                     DLDVEACGGTHVKKTSEIEQVKITKTKRIQDGVVRIEFVSGDTAKEFMLKKQQDVKSE
                     QEEEKIKELQKEKRFERKQIDKEKIPKIIEAFSQCAQGITTIDNIIMETTESTRPNFC
                     YSTNDDYNDDFHIRLGERLSKKDPNLVYCGLFNEGEKIRIIVYCGDQISKNKKAGDIV
                     KSVSEILGGAGGGTPKFAQGGGNDKSKKEDSIKKAKSMVLG"
     gene            15892..>17402
                     /locus_tag="QGF01_05895"
     CDS             15892..>17402
                     /locus_tag="QGF01_05895"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HJN20448.1"
                     /translation="MDVNWNTIDAKWQNKWAENNDHDVDPNEKEKKFITVAYPYPNSP
                     QHIGHGRTYTITDVHSRYLRMKGYNVLFPMGFHYTGTPILGMSKRVQANDTELINAFK
                     TLYNVPEDKIKDFVDPVKIADYFHEEIKAGMIEMGYSIDWRREFTTIIPAYQKFIEWQ
                     FRTLKQNNLIVQGNHPVGWCPNDQNPVSQHDTLGDVEPDFTEYVIMKFNIENEIIPIA
                     TLRPETLFGVTNLWINPEIVYKKTKVNDEIWIISSECAHKLSFLDKTIEIIGDVKGSE
                     LIGKNVKSPISSEEFLILPASFVSATTGTGIVMSVPAHAPYDYQALEDIKNGNTKGMP
                     ESIRDQVKAINPITIITTEGFGEVPAEDVIKRMNITNQNDPKLEDATKEIYSKEYYEG
                     KLNQNTDKFSGKRVSFVKDEIKEWFSKSGKSDIILELTNAPVKCRCGSECVVKMLNNQ
                     WFLDYGNKEWKEKAHNCFESMSILPNEIRTEFFNVLDWLRERACARQHGLGTRV"
BASE COUNT         6187 a         2796 c         2998 g         5421 t
ORIGIN      
        1 attctgctaa gcttagatct ttctgtatat catctccagt acagaaggta gagctactgg
       61 ttctggttca gctactggtt ctggttcagc tactggttct ggtttaccaa aaattgtttc
      121 aagaaatgta tctttattga aaggtttcag atcatcgtag ccctgtccag tgcctatgta
      181 tatgactggc gtcttggtga ctgcaacaat agaaatagct gcacctccac gaacatcggc
      241 atcactttta gttaaaattg ctccatcaaa atctgtatgt tcattaaatg ttcttgcctg
      301 actaacagta tcattaccag ccaatgaatc acctacaaat attttcaaat ccggattcac
      361 tacttttgaa atcttcgtaa tctgttccat caaattcttg tttgtttgca ttcttccagc
      421 agaatcaatc aatacacaat ctactttatg tgatttagcg taaagtaacg catcacgagc
      481 aaccgcagct ggatctgaac cataattttg tgcaactaat ttcagattca atttatttgt
      541 gtgttcacgc aattgctcaa tagcacctgc gcgaaaagta tcagatgcgg caactacgac
      601 tgatatttta tttttttgca aaagattggc gatttttgca atagtggttg ttttgccagt
      661 tccatttatg ccaataaata aaataagata tggctcctgt ggatttgttt tggatttgat
      721 attagacaaa atgtcaaccg agcctgcctc atcaaacata ttagaaatat tttgaatgag
      781 gctttgttta acaaaatgtt caatttcctt cttgtttacc ttactaccaa tcaatttttc
      841 agtcaaattt gattttatcc catctatgac ctcagttgca acatcagatt ctaagagcga
      901 gatttctaat tcggatatta catcttcgat gtccttttct ttgagttctt tttcccctaa
      961 actcttagca acatttgaaa atgcattgct tagtttatca aacatagttt tttctttatt
     1021 tttggttgtg tgcggtcatt aatttctcca tctctagttt cgcttgttgt aaaccttctg
     1081 aaacttgttg cttttgtcca gctgtttcct gtaaagctat ttgcatttcc tttaatcgcg
     1141 attcaagaaa attgatggca gaatttttgt ccttttctac agcaaccccc gcaccaatat
     1201 ttaggattaa ttttttgttt ggtgtaatct ttgtattgac aaaagtaccc aaacctacag
     1261 gcattagggt ttcattgtca tccttatcat taatagattt caggcattca atagcagatg
     1321 ttgcttctct aataatagac atgatcgaag actctttttg gtttaactca gaaaaatagc
     1381 cctcaagaac ttgtgttcta tagagtagtt gctgtgtctg ttcatcactc attataaaaa
     1441 aaatgcagta gttcagtata aatgcattta aggacttggt tagttttcaa aaataaataa
     1501 aaaatagaat tttattttgc cttagaaagt ctgttatcaa cttcatccca attaacaaca
     1561 ttccaccatg ctgcgatata atccggacgt ttgttttgat atttcaaata ataagcatgt
     1621 tcccaaacat caagacctaa caatggaatt aatccctcag tgcgaggact tgtttggttt
     1681 gacattgctt tgtattcaac tttgctggat gatggattat acacaagcca accccaacca
     1741 cttccttgaa ttggagccgt tttgctagaa aattgttctt taaagtcagc aaaagatcca
     1801 aaagatgcgt tgattgcatc agctaatact ccaccaggct ctccaccacc atttggcttc
     1861 atattattcc aaaatagttt gtgattatca tatccacctc cattgaagtt tattgcacct
     1921 cttacatcat caggaacatc atttatgttt gataaaatat caacaatatc cttgctctgt
     1981 agttccggac ttagtttttc taaagcacca ttcattccat cagtatattt ttgatggtgt
     2041 tttgtatgat gaatctccat tgtcctggca tcgatatgag gatctaatgc atcatatgca
     2101 taaggcatgt caggaagagt atattttccc atatcatatt ctaaaaacca taggattaat
     2161 aggtttggta gggaataaaa caatattaaa aaatgttatg atatggatgt tcgaaaaata
     2221 ttctttttaa ttgtcttcgt ttcaattggc atgattttat tatcaagtca attacaaaat
     2281 caagataata tatctataca agtaaatgat agtggatcat acattggaac tgacattcct
     2341 catagctatg gatatgatgg tacaggaata ataatttcag tgattgacac tggagtcgat
     2401 tttaaccacc cagatctttt gggttttggt tcagatggaa aagtaattgg tggttatgat
     2461 tttgttgata atgatcatat ccctgaagac gtcaatggtc atggaactca ggttgcagga
     2521 attatagcat caaatggtat actacaagga atagcaccaa actcaaaaat cttggcctat
     2581 aaagtttcaa aagatggcga atctgtacca tcaaacctga taattaaagc aattgaaaaa
     2641 tcaatcgaag atggtgcgga tataatcaat atcagtttag gagttaatca gactagtgca
     2701 aaaattgacc aagcagtaac aaaagcagtt caaaatggca taattgtagt tactgcagca
     2761 ggaaattttg gacctgagtt gagtaccatt gggagccctg gaataaatcc caatgcaata
     2821 actgtaggat ctactttcaa taatgtacca agtagcctag tttcaacact gaaaattaat
     2881 gataaagaat tcaatgtttt cccaatggta ggaacacaat atattgaaga atctatcgta
     2941 tcggaaatag tttttggaga gtatggaaga gtgaaagacc tatcagatat tgatgttcaa
     3001 aattcaattt tattaattga gcggggaagt gatatagaaa atgaaatagt tttcttttct
     3061 gacaaagaaa aaaatgcatc tgaattggat gcaaaagcaa ttattgttta taataatgag
     3121 ccaggtatat tctttggaga attaattcat gaatttgtag aagatgatta taatcctact
     3181 attccagcat tatctctatc aagagaagat ggactattca taaaagaaat gttaaaaatt
     3241 catatcgaag cagagttgga tgttttttac catcctgatt ttgttgcgta ctttagttca
     3301 agaggaccag tttctccctt ttacataaaa cctgacatgg tagcacctgg ggcatttatc
     3361 agtacaactg accttgctgg aaattacagt atctcaagtg gtacaagttt tgcagcacca
     3421 cacatttctg gtgtatccgc attgttatta caaaaaaatc cagaattgac atctaaagaa
     3481 ttaaaatcga ttctaatgac aacttctgat ctagtgtctg atgaatataa taaaagattc
     3541 cctcttgagg tatctggatc tggaagggtt aatgcgacta aagccataaa tgctgaactt
     3601 attatcacac cggcaaatct gatattcaat ttatcacctt atgatcaaac ggaaactaaa
     3661 attgtgaaaa taaaaggaat ggatgataaa tcattgtcag tcaagtttga gaataaccaa
     3721 gtgatagatc tgaaatatta ttttgagcaa aatgatctca taattacggc gaaattaatt
     3781 cagcatgatt taggtgaatt cgaagataga atgatcatta gtcatgataa cattgattat
     3841 caaataccaa tcctcctaca tgtttctgaa ggagcaatca caataaatga agatgatggt
     3901 gaattagata ttgttattgt caatccacaa tcatggtcat atgcaaaagt tacagctata
     3961 aacaaagata caggaaaaat attaactgac tctattgtac cgaataaaac atcagaattc
     4021 gttattcatg aacctggtga atactggatt gaagccaaaa ttgagtccga tgaaaataca
     4081 ctgaatgtgt ttgatactat caaaatagaa acggtatctg agaaaaattc gatcaacaca
     4141 ttaaattttc ctgagaaacc aattctgata atagctgcta taatgattgt aacagttatt
     4201 gtgggtctat ctataagaag aacttctaac taatcttcac gcttttggac ccgctttacg
     4261 tatttcgtca gaatcatcaa atctcttaaa attgtcaatg aacatctgag caagtttttt
     4321 tgctgaaaga gtataggaat ccttatcgat ccatgtgttt ttaggaatca aaacctctgc
     4381 aggtacgcct ggacattctg taggtatatc aagattaaaa atttcatcat gtctgtattt
     4441 tacgatgtct aatgcacctg ataatgcagc cttcaccata gcacgactat attttatgtt
     4501 aattcttttt cctacaccat aaggacctcc agaccaacca gtgttgatta aataaactac
     4561 cgtgttatgc tttgaaattt tttccccgag cattttagcg taaacacttg ctagtcgtgg
     4621 cataaatgga gcaccaaaac aaacagaaaa tgtagctctt ggttccttga ttcctcgttc
     4681 agttccagct aatttgctag tatagcctga catgaaatgg aacattgcag attccttggt
     4741 aagttttgaa actggaggta aaactcctaa tgcatcagcc gttaaaaata ctattacctt
     4801 aggatttcca ccaacgcttg gaataataga tcccggaata aagtccaaag gataagcagc
     4861 ccttgtattt tcagtcagag tcccatcatc aaaatcaggt ttcaatgatt ctttgtcaat
     4921 tacaacattt tctaaaactg caccagattt aatggcattc caaatttgtg gttcttcttc
     4981 ctcctttaga ttgatacatt ttgcatagca tccaccttca aaattaaata ttcctttatc
     5041 agaccatcca tgctcatcat ctccgatgag cattctttca ggatcagcag acaaacttgt
     5101 ttttcctgta cctgacaagc caaagaataa cacagtatca ttatcttttc ctatatttgc
     5161 tgaacaatgc ataggaaaaa ctccctttgt tggcatgaaa taattcatta ctgagaacat
     5221 agcttttttc atttcacctg catagtttgt tgcaccaatc agaactatct tttttgttaa
     5281 attgatcagt atgaacgcat ttgatcttgt accatgaatt tcaggtattg cgtcaaagtc
     5341 attgatacac attacagtaa actcgggttc gtgagattgt aattcagcct tcgaaggacg
     5401 aataaataat tgatgtacaa aaagattttg ccaagcatgg tcattgatta ctctaactga
     5461 taatctattt tcaggatcag cgccaataaa accatcaaat atgaatacct ccttattctc
     5521 aacagatttt ttcatacttt ctaaaatttt ttcaaatttt tcagatggga attgcttgtt
     5581 tatttttccc caatcaactg tgttgtgagt taattcatca taaataatga atctgtcctc
     5641 cggtgatctg cctgtgtaat ttccagtttt tactgataat gaaccggttg atgtgacaat
     5701 accctcattt ttttgtacag ccatttctac aagtttttct gctgaataat ttctatgaat
     5761 ttttgatggt tttatgccaa atttttcaat ctgagaagaa aatgcttcag acatattttc
     5821 actcatacca atcaccattt attaaaaata aattagtctt tatgcccatg agattttttc
     5881 gtgatgaata acgctattaa tctctttcta agtttatgtt ttttttgata tgatttagga
     5941 agatatttat ttcaaaattc aagatctctc gatatgccaa taaacaagga aaaattagct
     6001 cttcgcgatc aagaaaccaa aagtactcca aagttcataa gacctgaaag ctggagatat
     6061 aaaaaattaa aaactgcttg gagaaaacca aaaggagttg ataatcatca acgtaagcaa
     6121 aaaagtagag gtagaccggg tcttcctaaa gtaggttatc gtggtccaaa gtcagcacgt
     6181 ggattacatc catccggttt tacagataat ttagtttata atattaatga tttagatagc
     6241 ttaaacccaa aagaagatgc agtgagaata ggacatagcg ttggaacaaa aaaacgaata
     6301 gttatagtaa ctaaagcatt agaagggaaa ttcaaagtat taaatgcgag agtgaataga
     6361 gatgccagta aatcttagtg caaaaaagag actagtctca aggatgactg gggttggaat
     6421 caagagaata aaatttgatg ttgatcatct tgatgatgta gctgatgcaa tcaccagaca
     6481 acaaattaga agtttaatta cagctaacac cattagaatt aaacaaattg ttggtacgtc
     6541 tagaggaaga gcacaggaaa agaaaaacca aaaaaagaag cgtggtatga cacaaggttc
     6601 taaaaagggc cgaaaaggtg ctagagtagg caagaaagag gtctatgtaa caaaagtcag
     6661 atcacttcgt cgtagactca aaatagccaa agaaagaaaa gaaattacta ataagaattt
     6721 ttgggaaatt tatacaaaga ttcgtggaaa tacagttaga aatattgcac atctaagaac
     6781 attaatcgaa gagatcaaaa caaaaggaaa agattagaaa taaaattttt cgttatcaaa
     6841 atctataatt tttccatttt tgattttaat tccttctttc tccaacattt ttaattttac
     6901 tttttcacca taactatatc ctccaatttt accatcagac tttactactc tatgacatgg
     6961 tatgatgaca gggtatggat tctttttcat aactgttcca acaaatctct gtccattttt
     7021 taatcctaca gcttttgcca aatcactata agttgtaact tttccttttg gaacttgtaa
     7081 taattttcta taaactttga tgcttatact caaagtttga ttatctccaa atctgttcct
     7141 tcaagcattt caagaatttt atgtttattt ttgcccattc cagtccaatc taataatgca
     7201 gtagtagcac ctctatttct ttcaattata tgagaaaaca ggaattgatc tatttcatct
     7261 aatgcatgtt ttggaattac tgttccgagt gcatacttgc catgaattaa ttcactagta
     7321 aatttatcag aataatgtgt tcctccaaaa catattcctg taggatatga tttttgattt
     7381 tgtttcattg tctgtacaac tatttgtgcc actgaattac aaagatttac atcattccat
     7441 tcttttggtg tagttcctat ttcaatgaac aaagtaggtt tttctaatga agttggtccg
     7501 tgatgagttg cttcaattgt tatttgaaat ttagtaaatt ggtttctagc atcccataaa
     7561 ttttgaatgt atgatttttg aagattagag tgtggtatag aaacttgacg ttcataacca
     7621 ccaaaattag cagcagaaaa attaccagtg ttatggcaag ttaatgcaag aacgcctgat
     7681 tctgctgcat gttttgataa aaaaatataa ccgtcataat caaatttatc ttcaatccaa
     7741 tcagcggata tggctggagt tggtataatt gccaaatcaa aatattcacc cctaaaaaca
     7801 tccccatctt tgactaaatc acgtgaaaca aaattggcca tattaccacc tgctggatca
     7861 tcagcatagg caacaagtaa atccattaga taaaagatat attgacactc tattaagcac
     7921 cagcaaatga agtttaatgc tagacgtact atattaaatc tgaaaaatac tgtaaaactt
     7981 acaaaaaaat ctgataagga tgtatatatt cagcatctca gacttgtgct atttggaatt
     8041 gcagcagtag gtacattagg atttggtatt cagtttatat tttctgtttt tacatttggt
     8101 agataataat gtcagagtct gaacccatag atgagcaaaa accagaacca atgtctgaac
     8161 atgactctct cgatgaatta ctagcagaga aacctgcgtc acaaaaatca gatatagaag
     8221 agaaagaaag ccaggttgag gaagaagcaa aagttccagc agttgaacca gaatctggtg
     8281 atcaaacttc acatctattt gcagtaagaa ccacgggagg acaagaaaaa attgttatga
     8341 gattattaga agccaaaatg aggatggatc agaaagatga agaagaagta caaaaagcca
     8401 aagaggaggg taaagaaata aaaatcaaag acaagacttt gatagataaa gattggaaaa
     8461 aggcaggaga cttggaaaat tcactctatg tcgacgaaga atttcctagg gtaaaagatg
     8521 ttcaaaccgt acttttgctt gatgatttga aaggatatgt agttctcgaa gcacaagatc
     8581 caccttcgat gtttaatgca attcaaggaa ttagacatat tcgtggtcag ttaagaggtg
     8641 aattacagta tagtgagatc gacaaatatc taatcaaaaa atctacagtt acggaacttt
     8701 cagttgataa tactgtagag ataatcggtg gtccgttcaa aggtatgaaa gctactgtta
     8761 aaagagtaga tcaagaaaaa gaagaagctg ttgttatttt attagatgca ccttatcaat
     8821 taccagtaac tgtagatgct aatcatctca aaaaggtttt atcatagcaa ggattaaatt
     8881 ttcaaaaatt ggtgtcatac tatgggagat aaacaaacaa tatcatcatt ggttactggt
     8941 ggcgaggctt ctgctggtcc accattgggt ccagcattag gtcctatagg agtaaatatt
     9001 atggaagtca ttaatgctat caatgaaaaa accaaagaat tccaaggaat gaaaatacca
     9061 gttagtgtta ttgtagatac agaatctaag aaatgggaaa ttgaagtggg aattccatca
     9121 gcatctgcac taatactaaa ggaggctggt atacaaaaag gctcaggaac atctggaact
     9181 gaatgggttg gagaagtttc tgcagatatg attaccaaga tagctaatgt aaagttagaa
     9241 tcatcatatg ctagttcgct aaaatctgtt gcaaagcaga ttgttggtac atgccaaact
     9301 ctgggaatca aaattgaagg taaaacccct aaagaattca cttccgaaat taatgaaggt
     9361 aaatggaata gtaaattcga ataagatgaa aatttattat taaaaattag gaaatttttt
     9421 cgctctgtta gattattata ttttagtatc gtttatgaga taaaacggat ctttgtctgt
     9481 cttttgtaat tccacaaaag tctcagtgtt ttgtatacca tcaatttttc caattttgtc
     9541 tattacaacc atatgtaatt cctcaagatc tttagtatga acttgcatca tgatatcaaa
     9601 tcgtcctgtg acctctgaga tagaaattat tgcaggaaca cccattaatt ttttatgaat
     9661 ttctaccttg agttttggat ctcgatttat accaactgtg gctttaacat taattcccaa
     9721 taaagaatca tcaatgtcaa tcgtaaattt cttgattaat tttcttctct ctaatcgttt
     9781 aatcctacta tacaaaacag atgcattact tccaagtttt tttgaaagta gaggtactgt
     9841 aatatgtcca tctttggtta attcaaaaag tatcttcaga tctaattcat caagcccatg
     9901 caataatgac aaaatgaatg tattatggta ataaatgtag attttactag atttaactag
     9961 attatctcta aacgatttta aatgggtttt ttaaaaaaaa tctatggtta cagaatctca
    10021 actcgtagaa ctaatcaaac aggctaaaga gaatggtaaa gaacgcaaat tcaagcaatc
    10081 tctagaaatg ataatggtgt ttaaggatgt ggatgtaaaa aaaggatttg ctattaatga
    10141 gacgattcag ttacctcaaa aaatgggtga tgcagcacag gtttgtataa tagcatctgg
    10201 agatatggga gttaaagcaa agaatgcaaa tgctgacaga ataattaatg aagatgaatt
    10261 aactaaactt ggtgcaaata aacgagaatc aagaaaggtg attaacaaat atgatttctt
    10321 tttggcagat actaagctaa tgcctactgt aggtaaagtg ttaggacaac ttttaggacc
    10381 tagaggtaaa atgcctacac cagttccatt taatgctcca attgaatcat ttttagaacg
    10441 attcaaatca tccgtcagaa ttcgtgtaaa gaactcttta tccctatcat gtaaaattgg
    10501 agaagaagat atggatgaac agcatgttgc agcaaatgca aatacaatta ctaatgcagt
    10561 agaaaaaaaa cttccaaatg gagataaaaa tatcaaaaaa ataatgatta aaactacaat
    10621 gggtaaagca gtgaaattag aacaggtgaa aaaataaaat tgcacgagaa tagaaccaaa
    10681 ttcccaaaaa agaagatgcg gatgtatcaa atgatggagg aattaccaaa aaaatatagt
    10741 gtgactgcat tagtacgaat ggaaaaagta cgagcatctc aattattacc attaaggaaa
    10801 aaactcaaag gagaagtaga aattattagt ataaaggata aaattgcgca aaaagcattt
    10861 gagaaattag atatttctgg tattaaaaaa ttgaaagaaa atcttactgg tcaatgtctt
    10921 ttcatgttta caaacatgtc tccattcaag ctaaatgttt tacttggtaa aaacaaaata
    10981 atgcttgcag ccagaggagg tgatattgca agtatggatg ttgttgttcc accaaaaaat
    11041 acagggatag ccccaggtcc tatgctaact gagttcaagg aaaataaaat tcctaccaaa
    11101 attgatcagg gaacaatttg gattctgaaa gaaacaacac ctataaaaaa aggagaagtc
    11161 atatcaacca aattagcagc gttgttagga aaattagaca tcaaaccaat tgaagctgga
    11221 atagttttaa actctgcact gtctgaaggt gttctattta ctgaaaacga gcttgttgtg
    11281 gatgtagatt tattcagaaa acaaatttcc gaagcgcatc aaaatgctgt atcattgtca
    11341 attgaagcag catatattac aaatgagaat attgaacaaa ttttagcaaa ggcatctcaa
    11401 tctgcacgtt ctgtttcatt agaaataggc tatatgacag atgataataa agaacaggta
    11461 attcaaaagg ctgatggaca tgctaaaagt gttgtatcta agacaaaagg ttatactcca
    11521 acctaatcat aacgactttt aactctgggt aaattccaat tgtatttcat tgctactagt
    11581 cgtaatacag ttgttattac cataccaata atagttgctg cgtagatttc attagtaatt
    11641 aacagtgtta gataaaacaa aactgcaccg ataaaactag ccgatgcata caattccttt
    11701 acaaaaacaa ttggtgtttg atttacaaaa atatctctta gaattccacc tcctatagca
    11761 gtcaacattc cagataatac tatgacaagg aaattcatgc ctactaaatt ataagcaaat
    11821 gttgccccaa tcacagtaaa aataccaaga ccgagagcat caaattttag aaaaatattc
    11881 cagtgctgtc gcaattttga atacaagaaa aataatacta tcgctgaaat tatagtgatg
    11941 attacgtaaa caggatctat caaagagttt ggaagtgttt tacctaatat gatgtcacgt
    12001 atggtacctc cagcaacacc agtaattgtt gcaagaatta ttattccaac aatatcagct
    12061 ttatgctcaa ttgctttgaa tgctccagta actgcaaaag ccatggttcc aaataaatct
    12121 aaaatataga tgaagagttc tggagtagtg gaaagatcta ccaataatca gtgtacaata
    12181 gtcaagtaaa taacattaaa tgtttttgaa aattaaaatt agataattct aaccgaataa
    12241 agatgacaag ccttccattg ctttctcttc agatttagca cttgctgcct catcttgttt
    12301 tttgtcttct ttcttacctt ctccaccttc tgctggagct gctgctggag ctgctgcagt
    12361 tgctactgga gctgctttta tggcttcctc aatatctaca tcagccaaag atgctacaag
    12421 tgatttgact tgtgcctcat ttacttctac acctgatgcc ttcactattg aagtgatatt
    12481 ttcctcagtt acatctttct tcagtttgtg aagcattaat gcagcgtaaa catattccat
    12541 attacatttt tttttagtgt ataatataaa actatggaga actataatca gtttttgttc
    12601 aattcaggat tttgagactt ctacatattg cataaagcgt tctttttaga ttcttatcaa
    12661 ataaagtatc catccgttgt tttacaatcc gttttgcctc atctctttca gaaccaggtg
    12721 gtaaagaaag tacattattc aataattcag aaaatgattc acgcacccaa ccatctaaag
    12781 ctaagaagac gtttggagga atttccaaaa gctgtgattc attaagatct ataacaccag
    12841 tataattttc atgttctata cgataaatca atgcgtgtat acaattatcg ggatttggat
    12901 tttttagaat ttcaatagat cgttggcccg ccttaccctg ctctactttg ttcttcagcc
    12961 caataggatc cacaataact ccgttaatgc ctattttttt accgtttatt ccagtaactg
    13021 caccaacaat aaaatcatta tattttccat tagattgagt ggcaaaaaca aatgcgcctt
    13081 ctttaagacc ttttttcttt cttcccaaca tagggttaat ttgaaaacta ttctatttaa
    13141 tttattgtaa tcgacaattc ttaatatcgg tcaaccagaa tgttttgaat ggataaaaaa
    13201 gaaatacttt caaaattttc aactgatcct gacagatact ataaagtaaa attatttgaa
    13261 gatgttggat ttgagcgtaa atcatgtaaa acatgtaaga gattctattg gacactagat
    13321 gaaaatcgta taaattgtcc tgatcattct agtgacacat attcatttat cggcaatccg
    13381 ccaacaaaca agcggttcga ttatacacaa gcatggaaag aagttgaatc attttttgta
    13441 aaaaataatc atacatctgt aaatagatat cctgtcgtat gtaggtggcg tgatgacctg
    13501 tatttcacaa ttgcatccat tgtagatttt cagagagtta tgggaagtaa agtcatcttt
    13561 gagtttcctg caaatccact tgtagtacca cagacatgcc ttcgattcaa agatttggaa
    13621 aatgttggtg tcacaggaag acacttctca tcattctgta tgattggaca acatagtgtg
    13681 ccagacaata acggatattg gaaagatgaa tgcatcaatc ttgactttga cttactaaca
    13741 aaacaatttg gaattgacaa aaatgaaatt gtttttgtag aagatgtatg ggagggtggt
    13801 ggttcatttg gttcttcatt agaatatttt gttcgaggat tagaacttgg taatgccgta
    13861 ttcacagaat ttcaaggtga tttatcaaac tacaaaacat taggtcaacg tataattgac
    13921 atgggagcag gattagaaag atttgcatgg ataacaatgg gaactcctac tgcatacgat
    13981 tgttgttttg gaccaataac caaaaaccta attcaacagg taggaattga aacagattct
    14041 aatattttag ttccatattt tacagaaatc gcaaagaatt ttgaaatgta tgaagatttg
    14101 actctagtta gaaagaatgc aataaaaaat tctggcttgt ctgatgaaca aatacgtaaa
    14161 attgttactc cgctagaagg aatttacctt ataattgacc atgttcggac attgattttt
    14221 gccatatctg atggagcatt accaagtaat gttggtggag gatacaacct cagaataatg
    14281 ctacgaagaa ttatctcaac tatgaataga ctatcactac aatttgacat aaatgagatc
    14341 attgacacac agattgacta cttgaagaat acatatcctg aactcgaaaa aacacgtaat
    14401 gatgtcaaag aaatcattcg tattgaatct gaaagatatg gtatttcaaa gcaaagaatg
    14461 caaaaaattg ctactaactt gaacaaagat ccaacagttg atgacttaat cagactatac
    14521 gaatcagatg gagtcacacc agattatcta aaagaaatca aagtcatatc agaaattccg
    14581 tctacatttt atagtaaact ttcagagtta catcaaggta aaaagcaaaa agaacatcta
    14641 aacttaccat tagaaaacat ttcagataca gaacttttgt tttattcaaa cgacccaaaa
    14701 gaattcgatg ctaaagtttt gaaatcgttt gataattatg ttgtattaga cagaacatca
    14761 ttctatgcaa gaggaggagg tcaagaacca gatcatggga agattgataa ttatgaagtt
    14821 gtagatgtaa caaaacatgg agatattata attcatgaat taaaaggcgg aattccaaaa
    14881 caaggtgaaa cagtctcttg tatcataaat tcaactagaa gagatcgaat aacaaaaaat
    14941 catactagta ctcatgtgat taatacatca gcaagaagtg tattaggatc atgggtatgg
    15001 caacattcag ctttcaaaga cgaggatcat gcaagactgg acattacaca tcattccgca
    15061 ctcacagatg aagaagtctt acagatagaa caagctgcaa attcaattat tgagaaaaat
    15121 ataccagtaa aaattgaaaa ttttgacaga gggactgcag aacaaaaata tggatttaga
    15181 atttatcagg gtggagttgt tccagtaaaa tcagtacgta ttgtctctat tggagattta
    15241 gatgtagaag cgtgtggagg gacacatgtc aaaaaaacat ctgaaataga acaagtaaaa
    15301 attacaaaaa caaaaagaat tcaagatgga gttgtaagaa ttgaatttgt ttcaggtgat
    15361 accgcaaaag aatttatgct aaaaaaacaa caagatgtta agagcgaaca agaagaagaa
    15421 aaaatcaaag aactgcaaaa agagaaaagg tttgagagaa aacagataga taaagaaaaa
    15481 attccaaaaa taatagaagc tttttctcaa tgtgcgcaag gaattactac tattgataat
    15541 atcataatgg aaaccaccga gtcaaccagg ccaaatttct gttattctac taatgatgat
    15601 tataatgatg attttcatat tagattagga gaaagactat cgaagaaaga cccaaatcta
    15661 gtatattgtg gattattcaa cgaaggggaa aaaattagaa taatagtcta ttgtggagat
    15721 caaatttcaa aaaataagaa agctggggat attgtgaaga gtgtgtcaga aatacttggt
    15781 ggtgcaggtg gaggaacccc aaaatttgca caaggaggtg gaaatgataa gtctaaaaaa
    15841 gaagattcaa ttaaaaaagc gaaatcaatg gttttaggat agtgagatgt gatggatgta
    15901 aattggaaca cgattgatgc gaaatggcaa aacaaatggg cagaaaataa tgatcatgat
    15961 gttgatccaa atgaaaaaga aaaaaaattc ataactgtag catatcctta cccaaactca
    16021 cctcaacata ttggtcatgg cagaacttac acaatcacag atgtccattc aagatatcta
    16081 agaatgaaag gatacaatgt actatttcca atgggatttc attatacggg aactccaatt
    16141 cttggtatgt caaaacgagt acaagcaaat gatactgagt taattaacgc attcaaaact
    16201 ttgtataacg ttccagaaga taaaatcaaa gattttgttg atccagtgaa aattgcagat
    16261 tattttcatg aggaaattaa ggctggtatg attgaaatgg gatattccat tgattggaga
    16321 agagaattta caacaataat tcctgcatat caaaaattca ttgaatggca atttagaaca
    16381 ctaaaacaaa ataatctaat tgttcaagga aatcatccag ttggatggtg tcctaatgat
    16441 caaaacccag tatcacaaca tgatacgtta ggtgatgttg aacctgattt tacagaatat
    16501 gttataatga aattcaatat agaaaatgaa ataattccta ttgcgacatt acgtccagaa
    16561 acattatttg gagttacaaa tctttggata aatcctgaga tagtttataa aaaaacaaaa
    16621 gtaaatgatg agatatggat cataagttct gaatgtgcac acaaactttc atttctggat
    16681 aaaactatag aaattattgg tgacgttaaa ggttctgaat taattggtaa aaatgtaaaa
    16741 agtccaatca gttcagaaga atttttgatt ttacctgcat catttgtgtc tgcaactaca
    16801 ggaacaggga ttgtaatgtc ggtccctgca cacgccccct atgattatca agcgttagaa
    16861 gacatcaaaa atggaaatac aaagggtatg ccagaatcta tacgtgatca ggtaaaagcg
    16921 attaatccta tcacgataat tacgacagaa ggttttggag aagttcccgc agaagatgtt
    16981 atcaaaagaa tgaatattac aaatcaaaat gatccaaaat tagaggatgc aaccaaggaa
    17041 atttactcaa aagaatacta tgaaggaaaa ttaaatcaaa atacagacaa attttcagga
    17101 aaaagagttt catttgtgaa agatgaaatc aaagaatggt tttccaagtc tggtaaatca
    17161 gacatcattt tagagttaac aaatgcgcca gtaaaatgta gatgcggtag tgaatgtgta
    17221 gtcaaaatgc taaacaatca atggtttctt gattatggaa ataaagaatg gaaggaaaaa
    17281 gcacataatt gttttgaatc tatgagtatt ttaccaaatg aaatccgtac tgaatttttc
    17341 aatgttttag attggcttag agaacgtgca tgtgctagac aacatggact aggaacacgt
    17401 gt
//
LOCUS       DASCPJ010000122         3512 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001910971, whole genome shotgun sequence.
ACCESSION   DASCPJ010000122 DASCPJ010000000
VERSION     DASCPJ010000122.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001910971"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            69..338
                     /locus_tag="QGF01_05900"
     CDS             69..338
                     /locus_tag="QGF01_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20449.1"
                     /translation="MIPIFGTVLSILSSLSGQIGPSYDPLCYDVMLYIFGTMMFSIFL
                     LGIIGYWIRVHGWGYKDNRERWVDELDRHFENEGIGLIPDNGKYW"
     gene            410..559
                     /locus_tag="QGF01_05905"
     CDS             410..559
                     /locus_tag="QGF01_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20450.1"
                     /translation="MDENICQICSKDISKHTPEEWAKCLKAEDDAMIDKIKRHYSSKS
                     ENEKT"
     gene            complement(568..1116)
                     /locus_tag="QGF01_05910"
     CDS             complement(568..1116)
                     /locus_tag="QGF01_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20451.1"
                     /translation="MRWPGLGDPTKRRKTIRFLIILLAIGVSVGVTSSIIQGFLGQSD
                     PLKSCINDRDTPYKISATFELYVDKKKASVPANIGFDNVEEDGLFDSVCQRTLYTITD
                     DGTIYAEWEERYAFEIGHFLWVWDFPLKDMDLTKSRILVNGMESELFINSPLVQGQHY
                     KAEFTSKEYEESKDKDFLPPDL"
     gene            complement(1222..1794)
                     /locus_tag="QGF01_05915"
     CDS             complement(1222..1794)
                     /locus_tag="QGF01_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HJN20452.1"
                     /translation="MVDKKTTTLVFALGAVMVLGTLAPQLLVMVQTAEAHGVQAQLQS
                     RFIKIEDETFNRQSLATGETLTMSGKLVSLVERDQRGWVSLFSESTNSGNRWEIMARD
                     PPGVVFAIPGNAITPYKVTAKALEPGVYHVHTQLNVDKVGPGLGPGQTVVVEGEIIVK
                     PISFSMIWYQSIMIGAAYVVTFATRPWKVI"
     gene            1974..2333
                     /locus_tag="QGF01_05920"
     CDS             1974..2333
                     /locus_tag="QGF01_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20453.1"
                     /translation="MTLPKGFGSGGAGGASRADVEKMIGRRIENYTGLITLSYLGAFF
                     ATTAGTMVGYLYYPWAYASASGHYAMITLTVVEAIGYLFCVKVAEEGSTKKSNGQIAA
                     ALAGTTAVMLYVALYIS"
     gene            2467..3117
                     /locus_tag="QGF01_05925"
     CDS             2467..3117
                     /locus_tag="QGF01_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia monooxygenase"
                     /protein_id="HJN20454.1"
                     /translation="MVWLRRCTHYLFIVVVAVNSTLLTINSGDYIFYTDWAWTSFVVF
                     SIANTLMTVVGAVYYITFTGVPGTGAYYGLIMQVYTWVAKVAWFALGYPVDFVVHPMW
                     IPSCMLLDLAYWATKKNKHSLIFFGGVLVGMSMPLFNMVQLMMVADPLETAFKYPRPT
                     LPPYMTPIEPQVGKFYNSPVALGAGAGSVLSVTFAALGCKLNTWTYRWMAAWSKWD"
     gene            3294..>3512
                     /locus_tag="QGF01_05930"
     CDS             3294..>3512
                     /locus_tag="QGF01_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014177.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HJN20455.1"
                     /translation="MQLHSHEPKPFVKWAGGKRQLLPELVRNYPEKFDTYFEPFLGGG
                     AVMFNLLSKYPNLKCHVSDLNSNLILAYV"
BASE COUNT         1058 a          644 c          666 g         1144 t
ORIGIN      
        1 tgcgcagaat atgttaaatc tgaaaataac agaaatgtat atatgcattc caaccagtca
       61 ttttattgat gattcctata tttgggacag tactaagtat tctatctagt ctctctggac
      121 aaataggacc aagctacgat ccattatgtt atgacgtcat gctatacatt tttggcacta
      181 tgatgttttc aatattcctc ctaggaataa ttggatattg gattcgtgtt cacggttggg
      241 gctataaaga caatcgtgag agatgggtcg atgaactgga tagacacttc gaaaatgaag
      301 gaataggtct tattccagac aacggaaaat actggtaatc ttaccaatct tcattctttt
      361 tcattttata aataacatac ttttagtatt tcatattctt tccattttca tggatgaaaa
      421 tatttgtcaa atatgcagca aagatatttc aaaacatact ccagaagaat gggcaaaatg
      481 tttgaaggct gaggacgatg ccatgattga caaaataaaa cgtcattata gttcaaagtc
      541 tgagaatgaa aaaacctaat ttttgactta gagatctggt ggtaaaaagt ccttgtcctt
      601 agactcctca tattcctttg aggtaaattc agctttatag tgctgacctt gtactaaggg
      661 tgagttaatg aacaattctg actccattcc attgaccaat attcgtgatt ttgtcaagtc
      721 catatccttt agtgggaaat cccataccca tagaaaatga ccaatttcaa atgcatacct
      781 ttcttcccat tctgcataga tggtgccatc atcggttata gtatacaaag ttcgttgaca
      841 tacagaatca aaaagaccat cttcttcaac attgtcaaat cctatattag ctggtacact
      901 agctttcttt ttatcaacat ataattcaaa agtggcagat attttgtatg gggtatctct
      961 atcattgata catgatttga gtggatcact ctgacctaga aaaccctgta ttatactact
     1021 tgtaacacca acagaaacac caattgcaag aagtattata agaaatctta ttgtttttct
     1081 tctcttcgtt ggatctccta gtcctggcca acgcatacat aaaatagaat agatttgcat
     1141 aaagtcgttt ctatctcatt atttttggag ataaaatgga aaactagaat aaaaataaat
     1201 gaaaagaaaa aggtttgttg gttagattac tttccagggt cttgtagcaa atgttactac
     1261 atatgcagcg ccaatcatga tgctttgata ccatatcata gaaaagctaa ttggctttac
     1321 aataatttcg ccctcaacta ctacagtttg tcccggtcca agacctggtc ctactttgtc
     1381 tacattaagc tgagtgtgta catgatagac acctggctca agtgcttttg cagtcacttt
     1441 gtagggtgta atagcatttc caggtattgc aaatacaact cctggtggat ctctagccat
     1501 tatctcccat ctgttaccgg agttagtaga ttctgaaaat aaggaaaccc aacctcgttg
     1561 atctctttca acaagactta caagttttcc agacattgtc aaagtctcac cagtagcaag
     1621 ggattgtctg ttgaacgttt catcttcaat tttgataaaa cgactctgta attgtgcctg
     1681 tacaccgtgg gcttcagcag tttgtaccat cacgagcaat tgtggagcta acgtgccaag
     1741 taccataacc gcgccaagtg caaagactag agtagttgtt tttttatcga ccatagatat
     1801 cccgtataaa tcaaagaatt aaagatagga tataagtttt gacgaaaaac tcgatcatga
     1861 gagtgattta agcctaaaaa acagatagta ttttgaaaaa aattaccaat ttgaaaactc
     1921 atatcagaga tattaagaaa gatttttaag cattctctag ttataatcgg catatgactc
     1981 ttccaaaggg atttggtagt ggcggtgcag gcggggcttc ccgtgctgat gttgaaaaga
     2041 tgatcggtag acgtattgaa aattatactg gtctgataac actttcatat ctaggagcat
     2101 tttttgctac tactgctgga actatggtcg gatatctata ttatccatgg gcatatgctt
     2161 ccgcaagcgg tcactacgct atgattaccc taacagttgt tgaagctata ggatatctat
     2221 tctgcgttaa agttgcagaa gaaggttcaa ccaagaaaag taatggtcag atagctgcgg
     2281 ctttagccgg tactactgct gttatgttat acgtagcatt gtatatctca taatagaccc
     2341 actctctatc tttttcatat ttttaccaca ttttaccttc attttcctca cgaatcatga
     2401 gttgccagtt tatcgatcct cgaaatttca taaaatttta tatagtcaaa tcatcgctaa
     2461 aaattaatgg tctggcttag acgatgtact cactacctat tcatagtggt agttgcagtt
     2521 aactcaactc tgttaacaat taattcagga gactatatct tctatactga ctgggcttgg
     2581 acatcattcg tagttttctc tatcgccaat actttgatga cggtagtcgg agctgtgtat
     2641 tacattacat ttacgggtgt tcctggtaca ggcgcgtatt acggcttaat aatgcaggtc
     2701 tatacatggg tcgctaaagt tgcatggttt gcacttggtt atccggtgga tttcgttgtt
     2761 catccaatgt ggattccatc atgcatgtta ttggatttgg catattgggc cacgaagaag
     2821 aataagcact cgctgatatt ctttggtgga gttttagtgg gaatgtcaat gccgcttttc
     2881 aatatggtac aattgatgat ggttgcggat ccgctagaaa ctgcgttcaa atatccaaga
     2941 ccaacgttgc ctccatacat gacgccgata gagccgcagg tcggtaagtt ctacaacagc
     3001 ccggtagctt tgggtgctgg tgcaggatcg gttctgtcag taacgtttgc agcattgggt
     3061 tgtaagctca atacttggac gtatagatgg atggccgcat ggtcaaagtg ggactaaaat
     3121 acccaaaatc cctttttcat tttctttttt ctaattatgg gtaattatcg aacgtaacga
     3181 agtttgtatt taagctattt gccctagatc aaataattct ctaccctttg tgatcttttg
     3241 atgacttaga attacaaaac aattctttga catgatgaag tctgactcaa caaattcaac
     3301 ttcattcgca cgaaccaaaa ccatttgtaa aatgggctgg aggaaaaagg caacttttac
     3361 ccgaattggt caggaattac ccagaaaaat ttgatacgta ttttgagcca tttcttggag
     3421 gtggtgcagt gatgtttaat ttgttatcaa agtatccaaa tttgaaatgc catgtatctg
     3481 atttgaactc taatttgatt ttagcatatg tg
//
LOCUS       DASCPJ010000123         2165 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001916735, whole genome shotgun sequence.
ACCESSION   DASCPJ010000123 DASCPJ010000000
VERSION     DASCPJ010000123.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001916735"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="QGF01_05935"
     CDS             complement(<1..419)
                     /locus_tag="QGF01_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HJN20456.1"
                     /translation="MNMANITPINYEIEFEPLFHNFTFNGIEIITIDISKPTNLILLD
                     AAELKIKKSHVIQGRKTVTVITSLDKKNEKLTIKLAKKIKGKVKLCIEFTGILNDRLL
                     GFYKSQYKDQDGKTKYLATTQFEAADARRAFPCWDEPS"
     gene            659..928
                     /locus_tag="QGF01_05940"
     CDS             659..928
                     /locus_tag="QGF01_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20457.1"
                     /translation="MYECNANDRNELESIQGQILERIQYLRERNLDITTDEILLDYYS
                     FNDEVISCILDDHSFSPIIFGIMAVVGVFILYRIWKLRKKKFKRF"
     gene            951..1373
                     /locus_tag="QGF01_05945"
     CDS             951..1373
                     /locus_tag="QGF01_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HJN20458.1"
                     /translation="MKQKTASRKIKILVAKLGLDGHDRGALVLCRGFRDAGMEVIYSG
                     LFATPDRIAQIAEDEDVDAVALSLLNGAHGTLFPKVVKVLKKKGIKDVLVVGGGTIPE
                     EDKKALEKSGVSGNFGPGTSLDDIINHIKNGVSKLRKL"
     gene            complement(1374..>2165)
                     /gene="ilvC"
                     /locus_tag="QGF01_05950"
     CDS             complement(1374..>2165)
                     /gene="ilvC"
                     /locus_tag="QGF01_05950"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HJN20459.1"
                     /translation="ACKKADIIHILIPDMVQSKVYKDEIEPNLSEGNALAFSHAAAIY
                     WGWIKAPDNIDIIMIAPKGPGSKVRETYLDNFGTPSIVAVEQDKTGTAWDRTLGIAKG
                     IGSARAGLIKTTFKEEVETDWFGEQADLCGGSASMVVNAFETLVEAGYQPEIAYFEVL
                     HELKLIVDMIQKYGINGMWRRVSETARYGGLTRGPMVMNNQTKEQMKNVLQQIQDGTF
                     NKEWLDEYQKSGKDAFDKYMKQLDSHQIEKIGKQMRKMMWPDSTE"
BASE COUNT          666 a          357 c          367 g          775 t
ORIGIN      
        1 gatggttcat cccaacatgg aaatgctctt ctagcgtcag cagcctcaaa ttgtgttgtt
       61 gcaagatatt ttgtttttcc gtcttgatcc ttatactggc ttttgtaaaa tcccaaaagt
      121 ctgtcattta aaatcccagt aaattcaata catagtttta cttttccctt gatttttttg
      181 gcaagtttta ttgtcagttt ttcatttttc ttatccaatg atgtaattac agtgacagtt
      241 tttctaccct gtattacatg agatttcttt attttcagct ccgcggcgtc taacaaaatt
      301 aaatttgttg gttttgaaat gtctatagta atgatttcta taccgttgaa cgtaaaatta
      361 tgaaataatg gttcaaactc tatctcataa ttaattggag taatgtttgc catattcatg
      421 aatgttaaga atcattatct aaatttatgt caatagttat tacttatgtt tataactgca
      481 taaatttttt atcatttatt gaacgataaa gtggtaactg gtattgtaat cgttataata
      541 tcagtgattg gaatgtttct aggtctgaca caatttttcg gtgaagtgac tattacacca
      601 taacatacaa aactcataat tatgaaaagt ctattttcta tgtaatctaa tattattcat
      661 gtatgaatgt aatgctaatg acagaaatga gcttgaatcc atacaaggtc aaattcttga
      721 aagaatacaa tatttgagag aaagaaatct tgatattact actgatgaaa tattactcga
      781 ttattacagt tttaatgatg aagtgatatc atgtattctt gatgatcatt catttagccc
      841 aataatattt ggaataatgg cggttgtagg cgtgtttata ctttatagaa tttggaaatt
      901 aaggaaaaaa aaatttaaaa gattttaagg gaaatgtgtg aatacatact atgaaacaaa
      961 aaacagcatc tcgtaagata aagatccttg ttgcgaaact gggtttagat ggtcatgata
     1021 ggggcgcatt agttttgtgc cgtggattta gagatgctgg aatggaagtg atttattctg
     1081 gtttatttgc cacacccgat agaatagcac aaattgcaga agatgaggat gtagatgcag
     1141 ttgcattgag tttattgaat ggggctcatg gaacgctgtt tcctaaagtt gtcaaggtgt
     1201 taaagaagaa aggaattaaa gatgtacttg ttgtaggagg tggaacaatt ccagaagaag
     1261 ataagaaagc acttgaaaaa tctggtgtta gtggaaactt tggaccagga acatcacttg
     1321 atgatataat aaatcatatt aaaaatggtg tttctaaact aagaaaatta taatcattcc
     1381 gtagaatcag gccacatcat ttttcgcatc tgcttaccta tcttttctat ttgatgtgaa
     1441 tctaattgtt tcatatattt atcaaatgca tctttgccac ttttttgata ttcatctaac
     1501 cattccttgt tgaatgttcc atcctgtatt tgttgtagta catttttcat ttgttccttt
     1561 gtctggttat tcattaccat cggaccacgt gtaagaccac catatctagc ggtttcactt
     1621 acacgtctcc acataccatt gattccatat ttctggatca tatcaacaat aagtttcaat
     1681 tcgtgtaaaa cttcaaagta tgcaatctct ggttggtatc cagcttcaac caatgtttca
     1741 aatgcattta caaccataga tgcagaaccg ccacaaagat cagcttgttc accaaaccaa
     1801 tcggtttcaa cttcttcttt gaatgtagtt ttgattaacc ctgctcgtgc gcttccaatt
     1861 cccttagcaa ttccaagtgt tctgtcccat gcagttccag tcttgtcttg ttctaccgca
     1921 acaattgatg gtgttccaaa attatcaaga tatgtttctc ttaccttaga gcctggaccc
     1981 tttggtgcaa tcatgataat atcaatattg tcaggtgctt ttatccatcc ccaataaatt
     2041 gcagcagcat gcgaaaatgc taatgcatta ccctcagaaa gatttggttc tatctcatcc
     2101 ttgtaaactt ttgactgaac catatcagga attaagatat gtatgatgtc agcctttttg
     2161 caagc
//
LOCUS       DASCPJ010000124         1516 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001917134, whole genome shotgun sequence.
ACCESSION   DASCPJ010000124 DASCPJ010000000
VERSION     DASCPJ010000124.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001917134"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="QGF01_05955"
     CDS             <1..237
                     /locus_tag="QGF01_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HJN20460.1"
                     /translation="GFAAQTGGGLMLAIFAILGIPASTTHAITGSVMGAGAARRKRAV
                     RWKVSRQIIFSWIITIPGAAGLGIAFTYIIHLFV"
     gene            244..924
                     /locus_tag="QGF01_05960"
     CDS             244..924
                     /locus_tag="QGF01_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HJN20461.1"
                     /translation="MTSSNKIIFIAELIGTFGLVVGATGSIVYNEILGGIHGISLIAA
                     GHFIALTIVVYAFGKYSMAHFNPAVTIAFFITKHVSAKHLPVYFTAQAIGAFLGSIFV
                     LFVIGNHANIGTNTPNLEHDWASIIGIEIIATVFLMWVIYIVVSNKKLAKLSGVAIGG
                     IVALDVLFFGEVSGASMNPIRSLAPAIISGTPGDLWLYWTTPFTGTMLVAVVYNLLSR
                     RKKSTSTH"
     gene            960..>1516
                     /locus_tag="QGF01_05965"
     CDS             960..>1516
                     /locus_tag="QGF01_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJN20462.1"
                     /translation="MGSKSKKLFGEARKIIPSGVNSPVRFYKPYPFFVKKSKGDTIWD
                     EDGKRYIDYCNGYGALLLGHARREIISAVSSQLKKGTLYTIPTALEVDLSKLIRKNFP
                     SMDKIRLVNTGAEATMTAIRLARGFTKKKKIIKFEGCYHGAYSSVLVQAGSGSAHIGI
                     SVSEGGLKEISKNTLVVPYNDSDTL"
BASE COUNT          482 a          248 c          319 g          467 t
ORIGIN      
        1 gggtttgcag cgcaaaccgg tggaggactg atgttagcta tttttgcaat attaggaatt
       61 cctgcaagta caactcatgc aattactggc agtgttatgg gagcaggtgc agcgcgaaga
      121 aaacgtgcag ttagatggaa agttagcaga caaataatat tttcttggat aattacaata
      181 cctggagctg ctggccttgg aattgcgttt acttacataa tccatttgtt tgtataggta
      241 gtgatgactt catcaaacaa aataattttt atcgctgaac ttattggaac ttttggtttg
      301 gtagttggtg caactggttc tatagtttat aatgaaatac tgggaggaat tcatggaatt
      361 agtcttattg ctgccggaca tttcattgca ttaaccattg tagtttatgc gtttggaaaa
      421 tattcaatgg cacactttaa tcccgcagtg acaattgcat ttttcataac aaaacatgtg
      481 agtgcaaagc acctgccagt ttactttact gctcaggcaa tcggtgcttt tttgggaagc
      541 atctttgtat tgtttgtaat aggtaatcat gcaaatattg gaacaaatac accaaactta
      601 gaacatgatt gggcatcaat cattggaatt gaaattattg caaccgtatt cttaatgtgg
      661 gtaatctata tcgtagtaag taacaaaaaa cttgcaaagc taagtggagt tgcaataggt
      721 ggaattgttg ctttggacgt tttatttttc ggtgaagttt caggtgcttc aatgaaccca
      781 atcagatccc tcgcgcctgc gattatttcg ggaactccag gtgacctttg gctatattgg
      841 actactcctt ttactggaac aatgctagtc gcagtagttt acaatttact ctcaagacgt
      901 aaaaaaagca ccagtacgca ttagaaagat attagtgaga tttaacacga attttgtaaa
      961 tgggctctaa atcaaaaaaa ctctttggag aggctagaaa gatcattcct tcaggagtaa
     1021 acagtccggt gagattttac aagccttatc ccttctttgt taaaaaatca aaaggggata
     1081 caatctggga tgaagatggg aagcgctaca ttgattattg taatgggtat ggtgccctct
     1141 tgcttggaca tgcaagaagg gagataattt ccgcagtttc ttcacagcta aaaaaaggaa
     1201 cattgtatac aattccaact gcacttgaag tcgatctttc aaaactaatc agaaaaaact
     1261 ttccttcaat ggataaaatt cgtctcgtta acactggtgc agaagcaaca atgacagcaa
     1321 taagattagc aagaggattt acaaaaaaga aaaaaattat taagtttgaa ggatgttatc
     1381 acggggcata cagttcagtt ttagttcaag ctggttctgg gtctgcacat attggaattt
     1441 cagtttctga gggtggatta aaggaaatat caaaaaacac actagtagtt ccctataatg
     1501 attcagatac acttga
//
LOCUS       DASCPJ010000125         1850 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001951456, whole genome shotgun sequence.
ACCESSION   DASCPJ010000125 DASCPJ010000000
VERSION     DASCPJ010000125.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001951456"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="QGF01_05970"
     CDS             complement(<1..328)
                     /locus_tag="QGF01_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HJN20463.1"
                     /translation="MRLLIGGSPSKFFHLKEFGEKLKAKGHQYKLVKDTDIFTGFPSR
                     HVKDWFDNKEKFKQLISEFSPDAVFVDRQTNFGKTAIEEKIPLFVHLRGDFWAESEMA
                     KKNTLQI"
     gene            complement(325..1080)
                     /locus_tag="QGF01_05975"
     CDS             complement(325..1080)
                     /locus_tag="QGF01_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20464.1"
                     /translation="MKKDVVVGLGEIGNPILKILSKKNIVVGFDLNHNLMDGIKFEKY
                     KNLKTSFLHIAIPGTDKLITNILKLYKKFQPECIIIHSTIKPGTTEKLQKKIPIPVIY
                     SATRGVHKRMIYDLKRYIKFFVISANAPRSKWASSRYVKVMKDCGIKTKKMSSPETLE
                     LAKIICDTSYLGWLVNYAQLSNMIAIEYGVDYDEMWDFSHEIHKFLGNRPKMFPGIIG
                     GHCVIPNLDLIHNRSLNIIKEMNDIYEKKLENK"
     gene            complement(1183..>1850)
                     /locus_tag="QGF01_05980"
     CDS             complement(1183..>1850)
                     /locus_tag="QGF01_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20465.1"
                     /translation="LLIKNNKKQDKALLIIGFSLLIPAVYSYALASDSRFLFPLYPLL
                     CIAAIYTIKKMFELFHKEKILSVIIISSFIVISAYYLSLKDIDVEHELEAYDMSKLIA
                     DRVEGIYLYGPDAGYLHLVQLNQINEFPVNSDEYFPSGPIQVRATYNTMSEYIEQAKV
                     DGVTHLILDGRDNNPKLFNDVFYEREKYTFLIKDFSSDDYDYNYLVKIYRIDFEEFDR
                     LKI"
BASE COUNT          615 a          303 c          228 g          704 t
ORIGIN      
        1 atatttgtaa agtgtttttt tttgccattt ctgattctgc ccaaaaatct ccccttaaat
       61 gaacaaatag tggtattttc tcctcaattg cagttttgcc aaaatttgtc tgtctatcaa
      121 cgaatactgc atcaggtgaa aattcagaaa ttaattgttt aaacttctct ttgttatcaa
      181 accaatcttt gacatggcga cttggaaaac cagtaaaaat atcagtatct ttaacaagtt
      241 tgtattgatg accttttgct tttagcttct ctccaaattc tttaagatgg aaaaattttg
      301 acggagaccc tcctattaat aatctcattt attttctagt tttttctcgt agatatcatt
      361 catctcttta ataatattca aacttcgatt atgtatcaaa tccagatttg gaattacaca
      421 atgaccgcca attatacctg gaaacatttt tggtctgttt cctaagaatt tatgaatttc
      481 atgtgaaaaa tcccacatct catcatagtc tactccatat tcaattgcaa tcatattact
      541 taattgagca tagtttacta accaacccaa atatgatgta tcacaaatta ttttagctaa
      601 ttccaaagtt tctgggcttg acattttttt tgttttaata ccacaatctt tcatcacttt
      661 tacatatcta gaagatgccc atttacttct aggagcattt gctgaaatta caaaaaattt
      721 aatgtatcgt ttaagatcat atatcattct cttatgaaca cctcttgttg cgctatagat
      781 cacaggaata ggaatttttt tttgtaattt ttcagttgtt ccaggcttta ttgtactatg
      841 aataataata cattctggct ggaatttttt gtaaagtttt aaaatatttg taatcaactt
      901 atctgttcca ggtatagcaa tatgcaaaaa tgaggtttta agatttttgt acttttcaaa
      961 ttttattcca tccattaaat tatgatttaa atcaaatcca acaacaatat ttttcttaga
     1021 taatattttt aaaattgggt ttcctatttc accaagtccc acaacaacat cttttttcat
     1081 tctctgattt tttgactttt aagttataaa ttaattgtgt aggtaaagtt agtgttatag
     1141 atagatttta atcagaatat tgatatcaaa caacaattaa ttctagattt ttaatctatc
     1201 aaattcttca aagtctatac gataaatctt aactagataa ttatagtcat aatcgtcaga
     1261 actaaaatct ttaatcaaaa atgtatattt ctctctctca tagaaaacat cattgaataa
     1321 ttttggatta ttatctctac catctagaat tagatgtgta actccatcaa ctttagcttg
     1381 ttcgatatat tcactcatag tattgtatgt agctcgtacc tgaataggcc cagatggaaa
     1441 atattcatca ctattcactg gaaattcatt aatttgattt aattgaacaa gatgcagata
     1501 gccggcatct ggaccatata gataaattcc ttctacacga tctgcaatta acttagacat
     1561 atcataagcc tctaattcat gctccacatc aatatccttt agactcaaat agtatgcaga
     1621 tattacaata aaacttgaaa ttattataac cgataaaatt ttttccttat gaaatagttc
     1681 aaacattttt tttatggtgt aaatggctgc tatacacaat aatggataca gtggaaaaag
     1741 aaatcttgaa tcagatgcaa gggcgtatga atatacggca ggtatgagca aggaaaatcc
     1801 tattattaac aatgccttat cctgtttttt attattctta attagtaata
//
LOCUS       DASCPJ010000126         5342 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001973475, whole genome shotgun sequence.
ACCESSION   DASCPJ010000126 DASCPJ010000000
VERSION     DASCPJ010000126.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001973475"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="QGF01_05985"
     CDS             complement(<1..241)
                     /locus_tag="QGF01_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HJN20466.1"
                     /translation="MGTATGIIKALNSGIKHIATKRFTFRYPEQKLKFVGDGYQFDPT
                     SGVGIAGYKGRHILFHDKCTGCNLCSIACEEIAEAI"
     gene            complement(241..1545)
                     /gene="nuoH"
                     /locus_tag="QGF01_05990"
     CDS             complement(241..1545)
                     /gene="nuoH"
                     /locus_tag="QGF01_05990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HJN20467.1"
                     /translation="MSVIAPKFRLGYLIKGVIDNIFWIILLVTLIGIPIVQIVLIYID
                     FPIIDGEIWNPFRVLDALTHPERALPLVVSFMQSDLFKVVAFPGFGFAALIAAATIFI
                     ERKMLAKLQLRVGPFYCGKVEGILQLMADGLKLISKEIIIPAKADKPIFWAAPVLFAA
                     TAGAFVALIPVAPGWVVADIDLGLLAVFAVIGFFPIITILSAWSSNSKFPFIGGIRAL
                     HQMVSFEIPLILSVLGIVMLTGTLNLAEIVQSQANFPWIIFMPVGAVVFFITILAELE
                     RIPFDLPEAESEIVAGWLTEFSGMMYGLVQLGTYLKLYAFAGLFVVLFLGGWNGPDIV
                     PPFPAEIVTDGITILPGALTVEIPGLPLLAHEMINGTIWFMLKTIGVILVILLPRGVF
                     PRIRIDMLLHIGWYKLIALSFVNIFIALALVYGGVIGPGGIL"
     gene            complement(1546..2679)
                     /locus_tag="QGF01_05995"
     CDS             complement(1546..2679)
                     /locus_tag="QGF01_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HJN20468.1"
                     /translation="MSAKLPPGLQLEKVDDRIMTLNVGPQHPGSGHMRIIIKVDGDYI
                     VHTDPDPGYVHRGEEKMAEYRNYITNIPHLERPVIHDSCNILYPYCLGIEELLGLEVP
                     ERAKYLRVIAAELNRCVYIQYWLAIYGIFLGHSTMFMWPTGDRELLIDLLEKMTGARV
                     THAYFVPGGIRNDVPPNFEDMCLRQVNYFEKRIKEYADVFYDNPILISRTRDTGVLSR
                     EDAIKLGTTGSVLRASGVDFDIRKKEPYDVYDELDFQSPTLKEGDSYARSKIPWLDMF
                     ESCRIIRDALKKMPKSGSVRVKLKPKPKGGDTEIYRRVESGRGALGMYIVSKGKPQPY
                     RLKISVPSFRNLIAMPHLLTGEKLGNMPAVYWSLNYWPVEADR"
     gene            complement(2683..3228)
                     /locus_tag="QGF01_06000"
     CDS             complement(2683..3228)
                     /locus_tag="QGF01_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HJN20469.1"
                     /translation="MSSDENNTNVEKTEEAPPVPEFENPLANLILKKFPSATIAFANP
                     DRTRVNVSKEEIIQTASFIRDELHFDHAESVSGVDYPDEKQIEVVYHLGSYDDPSLSK
                     QIMILATRVPREDNPHPGNDTTKLSSLYEIFPSVAFHERECFEMLGVYFEGNPDNRRL
                     LLPEDWADIPPLRKDFDIKGR"
     gene            complement(3237..3791)
                     /gene="nuoB"
                     /locus_tag="QGF01_06005"
     CDS             complement(3237..3791)
                     /gene="nuoB"
                     /locus_tag="QGF01_06005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HJN20470.1"
                     /translation="MVNDITVSKPKGLSVINQINSDNANVFVGKLGDVLYKAVGTPLG
                     MALNWGRLWSLWPVHIETACCSVEFGAASGPRYDVERFGIIEAFGSLRQCDLVVVQGT
                     ITRKMAPRLRLVYDQMPEPKYVIAMGACAITGGLYFDSYNVLPGIDGILPVDVYVPGC
                     PPRPETLIQGCMLLQEKIKRMKAR"
     gene            complement(3785..4117)
                     /locus_tag="QGF01_06010"
     CDS             complement(3785..4117)
                     /locus_tag="QGF01_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HJN20471.1"
                     /translation="MFGFAVVALAPALIISRMIAPRTKSNPVKFLPMECGQVPSGEGR
                     THFMMQYYAYILMFVIFDVMAIFLYAWGSTLLDLPKTATLPIIGFLGIMFAAMAYALY
                     QTKRKNIW"
     gene            4275..4748
                     /locus_tag="QGF01_06015"
     CDS             4275..4748
                     /locus_tag="QGF01_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HJN20472.1"
                     /translation="MVLNTGDTAPDFELADTDLKMRTLNEFKAKKVVLSFIVAASSPV
                     CETELCTFRDSWEEISNLGAQVIAISNDGPFANKAFAEKNNFNFPILSDYNSKAIRDY
                     DILSPDLLHLKDYNFAKRSVFIIDENGTVVYRWVSEDPLKEPNYQEIIDFLKKGN"
     gene            complement(4751..5263)
                     /locus_tag="QGF01_06020"
     CDS             complement(4751..5263)
                     /locus_tag="QGF01_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HJN20473.1"
                     /translation="MEFKLEDVEETLNGEIMNKISNNEYLIKIQNKEHQLQILNINSN
                     GMEFVLDNKFHNVKYVENHTAEMRIVIDGVSLTINMHTKLDKIVYKNTGNTDTGSGQI
                     NLRSQIPGKVVSVEVNEGDKIKKGDVVCVLESMKMQVSVKSHKDGEVKNLKVKEGNSV
                     NKNDILAEIE"
BASE COUNT         1699 a         1059 c          857 g         1727 t
ORIGIN      
        1 caatcgcctc agcaatttct tcacatgcaa ttgaacaaag attacatcca gtacatttat
       61 catgaaataa aatatggcgt cccttatagc ctgctatacc aacaccgcta gtaggatcaa
      121 attgatatcc atctcctaca aatttgagtt tttgttcagg atatctaaat gtgaatcgtt
      181 ttgtggcaat atgtttaata ccagaattta atgccttgat tatacctgta gcagttccca
      241 ttataatata cctccaggtc ctatgacacc accatatacc aaagccaatg caatgaagat
      301 gtttacaaaa gacaatgcaa ttaatttgta ccaaccaata tgtaataaca tatcaattcg
      361 aatacgtgga aaaactcctc ttggaaggag aatgactaga attactccaa ttgttttaag
      421 cataaaccaa attgttccat ttatcatctc atgtgctaat aatggtaatc ctggaatttc
      481 tactgtaaga gcacctggta gaatagtgat accatcggta acaatttctg cagggaatgg
      541 tggaacaata tcaggcccat tccaaccgcc aagaaacaat acaacaaaaa gtcccgcaaa
      601 agcatatagt ttgagataag taccaagttg gacaaggcca tacatcattc cagaaaattc
      661 agtaagccag cctgctacta tttcactctc tgcttccggt agatcaaatg gtattctttc
      721 aagttctgca agaattgtaa taaaaaatac aaccgcacca acaggcataa agataatcca
      781 tgggaagttt gcttgacttt gaacaatctc agctagattc aaagttccag ttagcataac
      841 aattcccaaa actgataaaa ttaatggaat ttcgaatgaa accatttggt gaagtgccct
      901 tatacctcct atgaatggga atttgctgtt agaagaccat gcagataaaa tagtgataat
      961 agggaaaaat ccaatcacag caaataccgc taataaaccc aaatcgatat ctgctacaac
     1021 ccaaccaggg gcaactggta taagtgcaac aaaggctcct gctgtagctg caaacaaaac
     1081 aggtgcagcc caaaagattg gtttatcggc ttttgccgga attataatct ctttcgagat
     1141 taattttaat ccatcagcca tcagttgtag gatgccttca acttttccac agtaaaacgg
     1201 tcctactctt aactgaagtt ttgctaacat ctttctttca ataaatatag ttgcagcagc
     1261 aatcaaagct gcaaatccga atccagggaa agctacaact ttgaaaagat cagattgcat
     1321 aaaagatact acaagtggca atgctctttc agggtgtgtt aatgcatcta atactctaaa
     1381 cggattccat atttcaccat caattattgg aaaatctatg taaatgagta caatctgcac
     1441 tattggaatc ccaattaaag ttactaggag aattatccag aatatgttat caataactcc
     1501 cttgattaag tagcctaatc gaaacttagg tgcaataaca gacatttatc tatctgcctc
     1561 cactggccaa taatttaaac tccaataaac agcaggcata tttccaagtt tttcacctgt
     1621 aagtaaatgt ggcatagcaa tcaaatttcg gaatgatgga acactgatct ttagtctata
     1681 aggttgaggt ttaccttttg atacaatata cattccaaga gcacctctac ctgattcgac
     1741 tctacgatag atttctgtgt ctcctccttt tggttttggt tttagcttta ctctaactga
     1801 accagatttg ggcatttttt ttagtgcatc cctaattatt ctacaacttt caaacatatc
     1861 aagccaagga attttggagc gagcgtaaga atcaccttct tttagagtag gactttgaaa
     1921 atctaattca tcataaacat catatggttc ctttttccta atatcaaaat ctacgccact
     1981 tgcacgtaaa acagaaccag ttgtaccaag tttaatggcg tcttctcgtg ataaaactcc
     2041 tgtatcacga gttctggaga taagaattgg attgtcataa aaaacatctg catattcttt
     2101 gattctcttc tcgaaataat ttacttgcct caaacacata tcttcaaagt taggaggaac
     2161 atcattccta attcctccag ggacaaagta agcatgtgtg acacgtgcac ctgtcatctt
     2221 ctctaaaaga tcgattagta attcacgatc tccagtaggc cacataaaca ttgtagagtg
     2281 accaagaaag attccataaa tggccagcca atactgtata tacacacaac ggttgagttc
     2341 agcagctata acccttagat attttgctcg ttcgggtact tccaatccaa gtaattcttc
     2401 aatacccaga caatatggat aaagaatgtt acaagaatcg tgaattacag gtctttccaa
     2461 atgaggtatg ttagtgatat aatttctata ttcagccatc ttttcttccc ctcgatgaac
     2521 atatccagga tctggatcag tgtggacaat ataatcacca tcaactttga taattattct
     2581 catgtgacca gaaccaggat gctgtggacc cacatttagt gtcatgatac gatcatcaac
     2641 cttttctaat tgtaggcctg gaggaagttt agcactcatt ttttatctac ctttaatgtc
     2701 aaagtctttt cttaatggag ggatatctgc ccaatcttct ggtaataata atcgtctgtt
     2761 atctggattt ccttcaaaat aaactcctag catttcaaaa cattctcgtt catgaaatgc
     2821 aacacttgga aaaatttcat ataaactaga taattttgta gtatcatttc ccggatgtgg
     2881 gttgtcttct cgtggtacac gtgtagccaa tatcattatt tgtttactca atgaaggatc
     2941 atcataggat ccaagatgat aaacaacttc aatctgtttt tcatcaggat aatcaactcc
     3001 acttacagat tcggcatgat caaaatgaag ttcgtcccga ataaaagagg cagtttgaat
     3061 aatctcttcc ttgcttacat ttacgcgagt tctatctgga tttgcaaaag caattgtggc
     3121 actgggaaat ttttttaaga ttaagttagc tagtgggttt tcaaattcag ggacaggtgg
     3181 tgcctcttct gttttctcaa catttgtatt attttcatca gaactcattt ttaacactac
     3241 cttgccttca ttctttttat tttctcttgc agtaacatac atccctgaat gagagtttca
     3301 ggtcttggag ggcatcctgg tacatagaca tctactggca atataccatc tataccaggt
     3361 aacacattat acgaatcgaa atataatcct ccagttattg cgcatgcacc catagctata
     3421 acatattttg gttccggcat ttgatcatag actaaccgta gtcgtggtgc catctttctt
     3481 gttattgtac cctgtacaac aacaagatca cattgcctta aacttccaaa agcctctata
     3541 ataccaaacc tttccacatc atatcttggt cccgatgctg caccaaattc tacgctacaa
     3601 caagcagttt caatatgaac cggccataaa gaccataaac gtccccaatt taacgccatt
     3661 cctaatggtg taccaacggc tttgtaaagc acgtcaccta attttccaac aaagacattt
     3721 gcattatcac tattgatttg gttaatcacc gatagtcctt tgggttttga tactgtgata
     3781 tcgtttacca tatatttttc ctctttgttt gataaagtgc atatgccatc gcggcaaaca
     3841 tgattcctaa aaatccaatt attggtaacg ttgctgtttt tggtaaatca agtaacgtac
     3901 ttccccaagc atacaaaaag atagccataa cgtcgaatat tacaaacatc aggatgtaag
     3961 cataatactg catcataaag tgggttcggc cttcaccaga tggaacttga ccacattcca
     4021 taggcaaaaa tttgactgga ttgcttttag tacgtggtgc aatcattctt gatattatga
     4081 gagcgggggc taaagcgaca acggcaaaac caaacataaa caaaacatta ctgtaccctg
     4141 caacagtttc acctaccaaa gcagcttgac tccctcctga gaatataaaa atctatgtag
     4201 aatttagctg atcgaaaatg gctaattaat tttcatatgt tataattgat atatgacatt
     4261 atggttttga taatatggta ctaaatactg gagataccgc tccagacttc gagcttgcag
     4321 atactgattt gaaaatgagg acgttaaatg agtttaaggc taaaaaagtg gttttgtcat
     4381 ttatcgttgc agccagttct ccggtttgtg agacagaact ttgtacattt agggattctt
     4441 gggaagagat ttcaaatctt ggtgctcaag ttatcgctat aagcaatgat ggtccgttcg
     4501 cgaacaaagc tttcgctgag aaaaataact tcaactttcc catacttagt gattataaca
     4561 gtaaagcgat aagagattat gatattttat cacctgatct tctgcatctt aaggactata
     4621 attttgcaaa acgttctgtc ttcataattg atgaaaatgg tacggtggtg tacagatggg
     4681 tttcggagga tccattaaaa gaaccaaatt atcaagagat tatagatttc ctcaaaaagg
     4741 gaaattgatt ttattctatc tctgctaaaa tatcattttt gttaacagaa tttccttcct
     4801 taacttttag attttttact tctccgtctt tgtgagattt taccgatact tgcatcttca
     4861 ttgattctaa aacacaaaca acatcacctt tttttatttt gtcaccttcg ttaacttcta
     4921 ctgatacaac tttgccagga atttgacttc tcagattaat ctgcccagaa cctgtgtcag
     4981 tgtttccagt atttttgtaa acaattttgt ctagttttgt atgcatatta attgtcaaag
     5041 atacaccatc aataacgatc ctcatttcag cagtgtggtt ttccacatat ttcacattat
     5101 ggaatttatt atccaataca aattccatcc cgttagaatt aatatttaat atttgaagtt
     5161 gatgttcttt attttgaatc ttaattaaat attcattatt tgaaatttta ttcataattt
     5221 ctccgtttaa agtttcttct acatcttcaa gtttgaattc cattttatat caatccatta
     5281 agcttttcca tagtgagttt gtcgtatttg atgattttat tttactttga aagaattctg
     5341 aa
//
LOCUS       DASCPJ010000127         9840 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001980834, whole genome shotgun sequence.
ACCESSION   DASCPJ010000127 DASCPJ010000000
VERSION     DASCPJ010000127.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001980834"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(40..1152)
                     /locus_tag="QGF01_06025"
     CDS             complement(40..1152)
                     /locus_tag="QGF01_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HJN20474.1"
                     /translation="MRLLEYQAKELFKEYNINTPDSISSTDIEKGRQDAKKIGYPFVI
                     KAQVPVGGRGKAGGIQKCHNEDEFELNYPQVLNMSIKGEKTRAILLEKMSEYEKEIYL
                     SLFLNRSKRCYTVIASADGGVEIESVKNQIIRDVGLGEVSKKIAAEIAKEIGFDEVTS
                     THFVDILQKLSKLTIEKEAELTEINPLVVLKDGSLLALDGKIMTDDNSNFRHNELLKY
                     REQTELEAKAEKSGFSLVELDGNIAVVGNGAGLVMSTFDMLSDNGGKPACFLDVGGGA
                     TEESVYEALTLISKMKKVKAILVNLYGGIVKTTIVASAFIKAYDNNLIDLPVYARLMG
                     AEADKSKIMLKDTKTKMFDSVEQAINGVVMEVTKTG"
     gene            complement(1196..1525)
                     /locus_tag="QGF01_06030"
     CDS             complement(1196..1525)
                     /locus_tag="QGF01_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20475.1"
                     /translation="MVKQISLDTWSIHHLTDLLKKGSIIVEKIGTPIILYRQTMEEEE
                     GSYEEVICSLTKNYVIEQLIISGGIIVPTVSQQLVFTLEEFPDRLLRKSKDLFLETVD
                     LLEKKLG"
     gene            complement(1796..4996)
                     /gene="ileS"
                     /locus_tag="QGF01_06035"
     CDS             complement(1796..4996)
                     /gene="ileS"
                     /locus_tag="QGF01_06035"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HJN20476.1"
                     /translation="MELSNEFNLKEIEQTTKNYLSKVDLKKLIDDSPDKMDKIMFIEG
                     PPTLNGEPHAGHLRGRVFKDLWYRFNTLNGKNVVFNAGWDTQGLPVELQAEKELGITN
                     GKSEITTQEQVENLVAQCKKIVAKFHEKWIDVDKKLGMSFNQENAYWTYKNEYIEREW
                     KLLRAAHENNILTEGYRVVAYCPSCQTSLSHSEVNQGYEIVQDPSLYYKVKLQDEDAY
                     FIVWTTMPFTLVTDAMVGFNPDQEYVYVETNNEVWIIGKTRLEEFMKEIKVEDYKTLK
                     TVNGADFEGKKYIHPLLDNISALEKMSKQENYHIAVAEKFVDVNTGTGIVHLSPANGE
                     DDYNIAMKRKVEIFSPIDDEVKFTDEAGVYAGMFVRDADEKIVQDVKEKNALVKIGKI
                     KHKYPLCWRSKHKLVWLARREYFYMLDRLGDKAINAAQKVEYFFDQPKNRFLEIIKEK
                     HPWCISRERFWGCPLPIWKCVECENIERLFSRKEIVDASDDLPDGPDFELHRPWIDRI
                     SIKCKKCNSKMQREEFVLDTWHNSGVAPFASLSDDEYGKKIPAPFFTEGIDQTRGWAY
                     TLLIENVILNNKDTAPYESFLFQGHVLDEKGNKMSKSTGNVLDASDLLTEYPVDLVRF
                     YFMWKSSPIETLNFSVKELMSRPYQILSTLFHIHLYFRQNSQYDVFDINQTTVSWAKE
                     KGLLGPTDTWLLSKLQRTVDNCTELNKKCKFHESAKAVEDFLINSLSQIYIPITKPEL
                     WDDDESKKNRRFAIYAIIANALKQLDILLHPFSPFISEYLYLTTFGNEKSILLENWPK
                     ADKSLVNGKIEESFDMMNDAVSVCAAARMKGKLKRRWPLNRAIICIEKGEREKMELLS
                     DLLHTQLNVHTCDIIELEHHTGLAELLEMKKADIPIVPKVDLQRKAIGPKAKQDLGKL
                     LEIFAETDAETIIESLDKNGTFTFDINGNKISLDTDDFIIDFDVQEGFAFFKRDNLIG
                     IISTVRNEELMSKGLLKDLARRLQSLRKERGYNPTDMLNTASILDLEQESLDMLKDKT
                     EELAFLVRVKQVNFTKTCKEYKDDDIDGQKIKIAVE"
     gene            complement(5005..5214)
                     /locus_tag="QGF01_06040"
     CDS             complement(5005..5214)
                     /locus_tag="QGF01_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20477.1"
                     /translation="MPEFVCEKCGKREFHENEETLKLTISVHNQFCRGKENQAHSFMH
                     RDDAHLEPMDPERANDEKDVPILNK"
     gene            5268..5390
                     /locus_tag="QGF01_06045"
     CDS             5268..5390
                     /locus_tag="QGF01_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20478.1"
                     /translation="MPEPNFAAFGLWSCLTIAVIVVTGLVYRIYRKNRNENVTS"
     gene            5371..5946
                     /locus_tag="QGF01_06050"
     CDS             5371..5946
                     /locus_tag="QGF01_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HJN20479.1"
                     /translation="MKTLHLEKHGLRGLAIAESFLQNSKKSILAGLVTTSDFIIDGFI
                     FGRSTVGGDDATDTILSMYTKLNRSDINFILISGIVISLYNIVDIKKISQKTELPVIG
                     VTYNDSDSIQDAIKHHFPNSYNSKLDQYSKLDSRKEITLHTSYNLYIRNEGCTVLEAK
                     QLLDKLTLQGSFPEPLRIAQLLAKTLLRSKF"
     gene            complement(5929..6120)
                     /locus_tag="QGF01_06055"
     CDS             complement(5929..6120)
                     /locus_tag="QGF01_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20480.1"
                     /translation="MGIICKGICDQLRVRIASKQPRYSVGYKRCTFCAAYFSTPDSRC
                     PCCKTKLRTRARSKLKFRS"
     gene            complement(6154..7035)
                     /locus_tag="QGF01_06060"
     CDS             complement(6154..7035)
                     /locus_tag="QGF01_06060"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJN20481.1"
                     /translation="MTKVGLIGTGMLGEAVGLHLLESGYDLNVYNRTKSKTKKLAENN
                     ATVFDSPKHMVESSDLIITCVKDADAVKEVLFGNDGITSGKYTEISVADMSTINPTSA
                     QEISKKLHTMKIKSLEIPVMGGPNVAINGGLVLMASGDKELFETHQKIFKTIANKIFF
                     LGESGSAHTIKLAMNMQISLLALSLSEGIILMKKAGIEPEKFLEILNSTYFGTGMSEK
                     KAYKMIKNEYEPTFTLKNLKKDLDIITKTAKDFDAVLPITERANKIYDDALAAGFGEL
                     DYTGVLAYIEKISRTKD"
     gene            7159..7320
                     /locus_tag="QGF01_06065"
     CDS             7159..7320
                     /locus_tag="QGF01_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20482.1"
                     /translation="MGSIKELLLKSMRDNPEIIKKMQNEESDETMKAILKMILDEAQK
                     DPEYSGVTH"
     gene            7360..7878
                     /locus_tag="QGF01_06070"
     CDS             7360..7878
                     /locus_tag="QGF01_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HJN20483.1"
                     /translation="MMLGNRILLFFILMAIFSAVFAISAEANLPEEEVEMIMEEFESM
                     VEGIDAFGIFLHNTALSLPMFIPGFGIIWGMFSAFSTGIAFAAIKSMNPLLEQIPALS
                     ILFMTPFGLMEVAAYSIAMSRSYMLIHKIIKKVSIRSDIPVTLVEIIIVVSLLLVGAY
                     VEYYMIEMSSQI"
     gene            complement(7879..8076)
                     /locus_tag="QGF01_06075"
     CDS             complement(7879..8076)
                     /locus_tag="QGF01_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20484.1"
                     /translation="MKNSELKKLVAEYNDLKRRSKNHDVSDELKIIERRYFHETGNMI
                     NDELDGKKLYDLYRHTTKLIE"
     gene            complement(8078..8605)
                     /locus_tag="QGF01_06080"
     CDS             complement(8078..8605)
                     /locus_tag="QGF01_06080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HJN20485.1"
                     /translation="MSKIVIISGSPRKNGKTQVLMKHVSDYIKQKNPDTKFINLSEGE
                     VDYYTGDGNHSESTREIVKNVTEADVWFVGTPIYNSFFSSALKNLFEYVDYKKTEGKT
                     AGLVVLASGNIGFTFVQTLLIQLMSYFKVIANPKTVFVTADTIENDEIISDDVKSRLE
                     RMVDQTLILASKAKS"
     gene            8645..8815
                     /locus_tag="QGF01_06085"
     CDS             8645..8815
                     /locus_tag="QGF01_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20486.1"
                     /translation="MTEHGNMGHLHEEIHHLEDELRILEFNRPYETQKLRELAAELYE
                     KKVQLAESELQF"
     gene            complement(9304..9690)
                     /locus_tag="QGF01_06090"
     CDS             complement(9304..9690)
                     /locus_tag="QGF01_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20487.1"
                     /translation="MRDSETFGIETTFAERAIEWMNEQAKKNNWKFEARTYNSEIKTK
                     NFGSFEMFSWIGDVKVARSIIVKVSKRFKAKVIEGGYKPKDKIFKKKKSEFGVIRKGE
                     RILGHLEFSASRLGNEMWTLEAEERK"
BASE COUNT         2968 a         1848 c         1422 g         3602 t
ORIGIN      
        1 ccatctttat ttttgtcacc ttttaacaat tcaaaaatat cagccagttt ttgtcacctc
       61 cataacaact ccgttaattg cttgctcgac ggaatcaaac attttcgttt ttgtatcttt
      121 taacatgatt tttgatttat ccgcttctgc tcccattaat cttgcataaa ctggtagatc
      181 aatcaaatta ttatcatatg ctttgataaa tgccgacgca actattgttg ttttaacaat
      241 cccaccataa agattcacta atattgcttt cacttttttc attttactaa ttaatgttaa
      301 agcttcataa actgattctt cagttgcacc gccaccaaca tcaaggaagc atgctggttt
      361 tccaccatta tcagataaca tatcaaatgt agacataact aatcctgcac catttccaac
      421 taccgcaata ttaccatcta attctactaa ggagaatcca cttttttctg cttttgcctc
      481 taactctgtc tgttctctat actttaacaa ttcattatgt ctgaaattag aattatcatc
      541 ggtcattatt tttccatcaa gtgctagtaa ggaaccatct ttgagcacta caagtggatt
      601 tatttctgtt aactctgctt ccttttctat agttaatttt gataattttt gtaatatatc
      661 tacaaaatgt gtactggtca cctcatcaaa tccaatttct tttgcaatct ccgccgcaat
      721 tttttttgaa acctctccca aaccaacatc tcgtatgatt tgatttttga ctgattcaat
      781 ttcaactcct ccatcagcgg aagcaattac tgtgtaacat cttttacttc tattaagaaa
      841 caatgacaga tagatttctt tttcatattc cgacattttt tcaagcagaa ttgcacgagt
      901 cttttcacct ttgatgctca tgtttagaac ctgaggatag tttaactcaa actcatcttc
      961 attatggcat ttttgaattc caccagcttt tccacgtccg ccaacaggaa cttgtgcctt
     1021 tatcacgaat ggatatccaa tctttttggc atcctgtctt cctttttcaa tatcagtaga
     1081 agagatacta tctggtgtat tgatattata ttccttgaat aattccttgg cttgatattc
     1141 taataatcgc acaaaaaatg atgattattt cagtttataa tcgtaagtgt ccacattagc
     1201 ccagtttctt ttccaacaga tcaacagttt ccaagaataa atccttactt tttctcaaaa
     1261 gcctatctgg aaattcctct aatgtgaata caagttgttg actaactgtt gggacaatta
     1321 tcccaccaga tataatcaat tgttcgatga catagttttt cgtcaaacta caaatcactt
     1381 cctcataaga tccctcttct tcctccatgg tttggcgata tagaatgata ggcgtaccta
     1441 ttttttctac gataatagat ccctttttca aaagatcggt taaatgatga atagaccaag
     1501 tatcaaggct tatctgcttg accatgatta catgatacat tttttgtata tatggtgatt
     1561 gtttagttat acgataacta ggagtgatta gtttttgata cgtttttgac tctttttgta
     1621 cgtaatagtc atctggaata aaaataaaag agacagaatc agaagataat aatgaatgaa
     1681 ttagttgctg ttctttgcgt tgcatttctt gctggcggct ggattatttt catgattggt
     1741 agaaaaaaac gacaaaaaaa tacatcttga gaaaacctaa tccgtaatat gataattatt
     1801 cgactgcaat tttgattttt tgcccatcaa tatcatcatc tttgtattct ttgcaagttt
     1861 tagtgaaatt gacttgtttg actcttacta aaaatgcaag ttcttcagtt ttatctttca
     1921 acatatccaa tgattcctgt tccaaatcta aaattgatgc tgtgtttagc atatctgttg
     1981 gattgtaacc cctttccttt ctaagagatt gtaatcttct agctaaatcc tttaacaggc
     2041 cttttgacat caactcttcg tttcttacag tagatatgat acctatgaga ttatctctct
     2101 tgaaaaatgc aaagccttct tgaacatcaa aatctatgat aaagtcgtca gtatctagag
     2161 aaattttgtt accattgata tcaaatgtaa aagtaccatt tttatccaaa ctttcaatta
     2221 ttgtttctgc atcagtttca gcaaaaattt ctaacaattt tcccaaatcc tgcttcgctt
     2281 taggaccaat tgccttacgc tgtaggtcca cttttggtac aataggaatg tcagcttttt
     2341 tcatttccag taattccgcc aatccagtat gatgctctaa ttcaataata tcgcaagtat
     2401 gtacgttcaa ttgagtatgc agtagatcag ataacaactc catcttctcc cgttctcctt
     2461 tttcaataca tattattgcc cggtttaatg gccaccttct cttcaacttt cctttcattc
     2521 gtgccgcagc acaaacagaa acagcatcat tcatcatatc aaacgattct tcaatttttc
     2581 cattaacaag tgacttgtct gcctttggcc aattttctaa aagtattgat ttttcatttc
     2641 caaatgttgt aagatacaga tattcactaa tgaatgggga aaatggatgt aataatatat
     2701 ccaactgttt taacgcatta gctataattg cataaattgc aaatcttcta ttttttttag
     2761 attcgtcatc atcccaaagt tctggttttg taattgggat gtaaatttgg cttaatgaat
     2821 taattagaaa atcttctact gctttggctg attcgtgaaa cttacatttt ttgtttagtt
     2881 ctgtacaatt atcaactgtt ctttgaagtt ttgaaagtaa ccaagtatca gttggaccta
     2941 acaatccttt ttcctttgcc caagaaacag ttgtttgatt gatgtcaaat acatcatatt
     3001 gactattttg tctaaaatac agatgaatgt gaaatagtgt gctcagaatt tggtatggtc
     3061 tagacattaa ctcctttaca ctaaaattca aagtttctat cggactagat ttccacataa
     3121 agtaaaatct tacaagatca acaggatatt cagttaatag atcagacgcg tcaagaacat
     3181 ttccagtact cttactcatt ttattccctt tttcgtcaag aacatgtcct tgaaatagaa
     3241 atgattcgta tggtgcagta tctttgttgt ttagaataac attttcgatt aataatgtgt
     3301 atgcccatcc acgtgtttga tcaataccct ctgtgaaaaa tggtgcaggg attttttttc
     3361 catattcatc atcacttaat gaagcaaaag gtgcaacacc actattatgc catgtgtcca
     3421 acacaaattc ttcccgttgc atttttgaat tacatttttt acattttatt gaaattctat
     3481 ctatccaagg tctgtgtaat tcaaaatcag gtccatcagg gaggtcatct gacgcatcga
     3541 caatttcctt tctcgaaaag agtctttcta tattttcaca ttcaacacat ttccatattg
     3601 gtaatgggca tccccaaaac ctttctcttg aaatacacca tggatgtttc tctttgataa
     3661 tctctaaaaa tctatttttt ggctgatcaa aaaaatactc aactttttgt gcagcattaa
     3721 tcgctttatc gccaagccta tcaagcatgt aaaaatattc tcttcttgca agccaaacta
     3781 gtttatgctt ggaacgccag catagtggat atttgtgctt aattttacca atttttacta
     3841 aagcattttt ttctttcaca tcctgaacaa ttttttcatc tgcgtcacgt acgaacattc
     3901 cggcatatac acccgcttca tctgtaaatt ttacctcatc gtcaatagga gaaaatatct
     3961 caactttacg tttcattgca atattataat catcttcacc attggctgga gaaagatgta
     4021 cgattcctgt accagtattg acatcaacaa atttttcagc aactgcaatg tgatagtttt
     4081 cttgctttga cattttttct aatgcagaaa tattatctag taatggatga atgtattttt
     4141 taccctcaaa atcagcacca ttcaccgttt tcaatgtttt ataatcttct actttaatct
     4201 ctttcataaa ttcttcaaga cgtgtcttac caataatcca gacttcgtta ttggtttcta
     4261 cataaacata ttcttgatca ggattaaatc caaccattgc atctgttaca agtgtaaatg
     4321 gcattgtagt ccagacaata aaatatgcat cctcatcttg tagtttcact ttgtagtata
     4381 atgaaggatc ttgaactatc tcataacctt ggttaacctc agaatggctt agtgaagttt
     4441 gacagcttgg gcagtaagcg actactctgt atccttctgt tagtatatta ttttcatgag
     4501 cagctcttag tagtttccat tctctttcaa tatactcatt cttgtatgtc cagtatgcat
     4561 tttcttgatt aaacgacatt ccaagttttt tatctacatc aatccatttt tcatgaaatt
     4621 tcgccacaat ttttttacac tgagcaacta aattttcaac ttgttcttgt gtagtaatct
     4681 cacttttgcc atttgtaatt ccaagttctt tttcagcctg taattcaact ggaagacctt
     4741 gtgtatccca acctgcatta aaaactacat tttttccatt taatgtgttg aacctatacc
     4801 ataaatcttt gaagacgcga cctctaagat gacctgcatg aggttcaccg ttcagtgtgg
     4861 gaggcccttc gatgaacatg attttatcca tcttatccgg tgaatcatct atcaattttt
     4921 ttaaatctac ttttgaaaga taatttttag tggtttgttc aatctctttc agattaaatt
     4981 cattagataa ttccatatcc gttctcattt gttgagaata ggtacatctt tttcatcatt
     5041 tgccctttca ggatccattg gttcaagatg agcatcatca cgatgcatga aactgtgagc
     5101 ttgattctct tttcccctac agaactgatt gtggacactg attgtgagct tcaacgtttc
     5161 ttcattttcg tgaaattcac gtttaccaca cttttcacag acaaattctg gcatatgttt
     5221 cactattttc taatgtattt attttattcc tatacctaga ttagagtatg ccagaaccta
     5281 attttgctgc atttggacta tggtcatgtc ttaccatagc tgttatcgta gttaccggtt
     5341 tggtatatag aatttacagg aaaaatagaa atgaaaacgt tacatcttga gaaacatggt
     5401 ctgagaggtc ttgcgatagc tgaaagtttt ttgcaaaatt caaaaaaatc aattcttgca
     5461 ggtcttgtta caacatcaga tttcattatt gacggattta tttttggacg ttccacagtt
     5521 ggtggtgacg atgcaacaga tacaatatta tctatgtaca caaaattgaa tcgatcagat
     5581 atcaatttta tattaatctc aggaatagta atatcactct acaacatagt tgatataaaa
     5641 aaaatatcac aaaaaactga attacccgtt ataggtgtaa catacaacga ttcagattct
     5701 attcaagatg caatcaaaca tcactttcct aattcataca actcaaagct tgatcaatat
     5761 tctaaattag attcacgtaa agaaattact ttgcacacat catacaattt gtatatacgt
     5821 aatgagggct gtactgttct agaagcaaaa caattgcttg ataaacttac attgcaaggt
     5881 tcgtttccag aaccgttacg aattgcacag ttattagcaa aaacacttct aagatctaaa
     5941 ttttaattta cttcttgcac gtgttcttaa ttttgttttg caacaaggac atctactgtc
     6001 aggtgtagaa aaatatgcag cacaaaaagt gcatcttttg taaccaaccg aatatcttgg
     6061 ttgtttagat gcaattctta ctcttaattg atcacaaatc cccttgcata tgatacccat
     6121 aatggaacta gtattttgta gtgattaata ttcttagtct tttgttctag atattttttc
     6181 tatgtatgcc aaaactccag tatagtctaa ttctccaaat cctgcggcta atgcatcgtc
     6241 ataaatttta tttgcacgct cagtaatagg cagaactgca tcaaaatctt ttgctgtctt
     6301 agtaattatg tccaaatcct ttttcagatt ctttagggtg aaagttggtt cgtattcatt
     6361 ctttatcatt ttgtaagctt tcttttcaga cattccagta ccaaaatatg tagagtttaa
     6421 aatttctaga aatttttctg gttcaattcc agcctttttc atgagtatga taccttcaga
     6481 taatgataat gctaacaatg aaatttgcat attcattgca agttttatgg tatgtgcact
     6541 acctgattct cccaggaaaa atattttatt agcaattgtt ttgaaaattt tctgatgtgt
     6601 ctcaaacagt tccttatcac cagatgccat cagcactagt ccaccgttaa tagctacatt
     6661 tggcccaccc attacaggta tttctagtga ttttattttc atcgtatgaa gtttttttga
     6721 tatttcttgt gcacttgttg gattaattgt tgacatatct gcaacagata tttctgtata
     6781 cttccctgaa gttattccat catttccaaa taatacctcc tttacagcat ctgcatcttt
     6841 cacacaagtg ataatcagat cagaagattc taccatatgt ttaggtgaat caaagactgt
     6901 ggcattattc tccgctagtt tttttgtttt tgatttggtt ctattataca cattaagatc
     6961 atatccagat tcaagcagat gaagtccaac tgcctcaccc aacattcctg ttccgatcag
     7021 accaaccttt gtcataaaac ttgattgtga tcaaattatg tatattcttt gatcatgtag
     7081 atcgatattc tattttacaa taagatgaaa tcctgtaaat tcttagtatt catatcatat
     7141 aacatcatca acaaactgtt gggcagcata aaggaattat tattaaaatc aatgagagat
     7201 aatccagaga ttatcaaaaa aatgcaaaac gaagagtctg atgaaacgat gaaggccata
     7261 ctcaaaatga tccttgatga agcacaaaag gatcctgaat attctggtgt aacccattaa
     7321 ttataataac tgaaaatatt ccatacatca aagtgtatta tgatgttagg caataggatt
     7381 cttttatttt ttattttgat ggctattttt tctgcagttt ttgcgattag tgcagaagct
     7441 aatcttccag aggaggaggt ggagatgatt atggaggaat ttgaatcaat ggttgaagga
     7501 atagacgcat ttggcatctt ccttcataac acagcactct ctttacccat gtttattcct
     7561 ggatttggca tcatttgggg catgttttcg gcattttcta ctggtatagc attcgctgca
     7621 attaaaagca tgaatccact tttagaacaa atcccagcat tgagtatctt attcatgaca
     7681 ccatttggat tgatggaagt tgcagcgtac tcaattgcaa tgtcaaggag ttacatgctc
     7741 atccataaaa tcatcaagaa agtgtcaatt cgaagtgaca tacctgtaac acttgttgaa
     7801 ataataattg tagtcagcct gttacttgtt ggagcatatg ttgaatatta tatgattgaa
     7861 atgtcgtctc aaatctaatt attctatgag ttttgtagtg tgacggtaaa gatcgtaaag
     7921 tttcttgcca tctaattcat catttatcat gtttccagtt tcatgaaagt atctacgctc
     7981 aattattttg agttcatcgg aaacatcgtg attttttgat cttcttttaa gatcattata
     8041 ttcagcaact aattttttga gttcagagtt cttcatgcta tgattttgct ttagatgcaa
     8101 gtattaaggt ttgatcaacc attctttcaa gcctagattt tacatcatca cttattattt
     8161 catcattttc aatcgtatct gccgtaacaa atactgtttt tggattagca atcactttga
     8221 agtacgacat gagttgtatt agtaaagttt gaacgaatgt aaaaccaata ttacccgacg
     8281 ctaaaactac tagacctgca gtttttcctt cagttttttt ataatcaaca tattcaaaga
     8341 gatttttcaa ggctgaactg aaaaacgagt tatagatcgg tgttcccaca aaccaaacat
     8401 cagcctctgt gacatttttt acaatctccc ttgtagattc actatgattc ccatcacctg
     8461 tataataatc aacttctcct tctgatagat tgataaattt tgtatcagga tttttttgtt
     8521 tgatgtaatc agaaacatgt ttcattaaga cctgggtttt tccatttttt cttgggctac
     8581 ccgaaattat tacaatcttg ctcatttcca tcttgtataa atatgggtat ttgttagttt
     8641 ttgtatgaca gaacatggga atatggggca tcttcacgag gaaattcatc acttagaaga
     8701 cgaattacga atcctagagt tcaacagacc ttatgaaact cagaaactac gcgaactggc
     8761 tgctgaactt tatgaaaaga aagttcaact agcagagtct gaattacagt tttaaaacaa
     8821 atatcagtat tttttcattg atctcgtatc cgttttatga tcaaaaatgc aactataccc
     8881 ccaactatag atccaattat tatgaattca gtgaaaaatg aatcattcat tgatattatt
     8941 atcgtagatg aataataaac tattttttat ttcaatttcc tgactaatta ttgaattatt
     9001 tttctttttt tataatttta acagtaaaaa aaattattac ctataaagta aacactgcaa
     9061 ccaaattaag cacgatcgca aaaaatgatc gtacttatgt attagctgga tctagcaaag
     9121 ctagacatgg atcaagattg atcacagtgc tcccctatac ggtgactgtc ttgattcatg
     9181 tatcttaagt taatctgctt tattagtgaa tcgtacgaaa ttcgcaacaa gaaaatttta
     9241 tgtcacggac tgatcagaaa atgttaaaac atgtttatta catatgttgt aaactgttga
     9301 cgatcatttt ctttcttcag cttctaacgt ccacatctca ttacccaatc gtgacgcaga
     9361 aaattccaag tgaccaagta ttctttctcc tttcctaatc acaccgaact ctgatttttt
     9421 tttcttgaat attttgtcct ttggcttgta gccaccttct attactttag ctttgaaacg
     9481 tttactgacc tttacaataa tacttctagc aacttttacg tcacctatcc acgaaaacat
     9541 ttcaaatgat ccaaaatttt tagtttttat ttcagaattg tatgttctag cttcaaattt
     9601 ccaattattt ttctttgctt gttcgttcat ccattcaatt gcacgttcag caaatgttgt
     9661 ttctatgcca aatgtttcag agtctcgcat gtataatatc taagacctaa ttgtacatga
     9721 atgttattag aaaaaattgc ctcctgaaga cagcgaaaaa aactgtcttc aaaggctaaa
     9781 tagggtttgt tcttggacct tcatctagaa tctgtccggc gttaccctca acacgcttac
//
LOCUS       DASCPJ010000128         7328 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001984063, whole genome shotgun sequence.
ACCESSION   DASCPJ010000128 DASCPJ010000000
VERSION     DASCPJ010000128.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001984063"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="QGF01_06095"
     CDS             complement(<1..848)
                     /locus_tag="QGF01_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248206.1"
                     /note="catalyzes condensation of pyruvate and acetyl-CoA
                     to form (R)-citramalate; functions in isoleucine
                     synthesis; belongs to the alpha-IPM synthetase/homocitrate
                     synthase family; it is difficult distinguishing these
                     proteins from enzymes in that family; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJN20488.1"
                     /translation="MNDPNQYAEEYNAVEKEPKRIRILDSTLREGEQHPGVSFTIKQR
                     IQIAWMLDYFGVDQIEISPIISNDHAEATKTIIKQGLSADIVAHGRALKEDIDVSLKC
                     DATWVAAYLGISDIHLKDKLRISREEALDRAVKTVEYAKSHGLKIRFTVEDGCRADPQ
                     FLIKVCKAIEEAGVDRISLPDTVGVLRPTGMSNFVKTVRSEVNVPLDAHVHNDIGFAL
                     ANAFAACDAGAANVRLINFIVATSIGIIPGTISFVLLGSSTKNTTHLILAAALFFIGM
                     YVSQKI"
     gene            complement(964..1374)
                     /locus_tag="QGF01_06100"
     CDS             complement(964..1374)
                     /locus_tag="QGF01_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJN20489.1"
                     /translation="MYRDNTDEKILGFLRDDSRESFVVIGKKLKLSESAVRRRVKNMV
                     NNGVIEKFTIEEQNASAIVLVSVDSSIDTSKVSVKLTKLNAVKTVYEITGQYDISVII
                     KAPNITEINACIDDLRKIPGVIDTNTVIILRTIR"
     gene            complement(1367..2542)
                     /locus_tag="QGF01_06105"
     CDS             complement(1367..2542)
                     /locus_tag="QGF01_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HJN20490.1"
                     /translation="MSEDQYLGNLYQRFPVVIEKGLGSHVWDSNGKEYIDCMGGYGVA
                     LVGHRNERVVNAIKSQIEKIITVHSSLYSKTREEFLENLFKIAPKGLTQVHLNNSGAE
                     SVEAAIKFARKFTSKKGMIAMKGSYHGKSMGALSLTFNPKYRESFKPLVEGAKFSAYG
                     DLDALRDSVDEDTAFIILEPIQGESGIHVPPDGFLQGVRKICDENEILLIFDEIQSGL
                     GRTGRMWASEHWETTPDIMCLAKGIAGGIPMGATLVKSDILSVMKKGEHSSTFGGNPV
                     SCAAGTATIQALTQDNLLKNAVMMGEKLFQGLTEMKSKHSIIREVRGKGLMIGVELKF
                     EVKDILLEGIKNGLLLLYSGRNILRFLPPLVISDEDVTNTLQILDKLLTNEEGRKNV"
     gene            complement(2535..3338)
                     /locus_tag="QGF01_06110"
     CDS             complement(2535..3338)
                     /locus_tag="QGF01_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate/[LysW]-glutamate kinase"
                     /protein_id="HJN20491.1"
                     /translation="MITIKIGGSVVDDLHPSVVADIKKVVEQEGVILVHGGGKEVTKV
                     TEQLGKEPKFVVSPGGVKSRYTDLETAEIFTMVMSGRINKTIVRMLQKNGINAIGLSG
                     IDGKIIQANRKKKLIIMNEKGRKQAIDGGYTGKITQINAQLLTTILNQGYTPVISPIA
                     ISEENDFLNVDGDRAAANVAGHVKSDKVLFLTNVDGLLMDEKLVDTLTLAKAKEILPQ
                     IGFGMEKKILAATEALEMGVKEALIANGQRENPITSAIQHHTCTVIKNE"
     gene            complement(3342..4394)
                     /gene="argC"
                     /locus_tag="QGF01_06115"
     CDS             complement(3342..4394)
                     /gene="argC"
                     /locus_tag="QGF01_06115"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HJN20492.1"
                     /translation="MKVGIVGASGYVGGEVVRLLLSHPEAEVSMVTSTKHVGEYLHRI
                     HPSLKGFTELTFSELDYDKMSDKCDLVFTAVPHGTATDIVKAFYDRGMKIIDLSADYR
                     LHNPEDYTKWYGWEHPHPDYLEKSVFGVPELHRDKIKNAQLVSCPGCMAVTSTLALYP
                     LIKNDLIDIDHIIVDSKIGSSGAGAGGTSGTQHAMRAGVIRPYKPAKHRHTGEIEQEL
                     SEVAGKKIKVSMSPHAVDIVRGILCTNHTFLKKEVSEKDLWKIYRAQFGEEQFIRLIR
                     DKKGLYKFPDPKFVVGSNFCDIGFDLDEDNNRLIALSASDNLMKGAAGSAIQNMNVMS
                     GFDEKQGIMYSPLTPV"
     gene            complement(4440..5297)
                     /gene="lysX"
                     /locus_tag="QGF01_06120"
     CDS             complement(4440..5297)
                     /gene="lysX"
                     /locus_tag="QGF01_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HJN20493.1"
                     /translation="MSKICIVFDRLRSEEKLLEKQAIDLGNETKMVDAKITHFDTDSN
                     KQNTDFGDVVLERCISYYRGLHFTACIEFLDVPIINKFSVSSLCGNKMLTSIILKKNN
                     IPTPKTYFSFSADAALENIEKIGYPLIIKPVIGSWGRSVIPLKDRDTADAIIEQRGIT
                     DGPLDRIFYLQEVVDRPPRDIRVITVNDRPIAAMYRKSSGGFKTNVALGADPEICDIT
                     NEIEDLCAKASKAVGGGILGIDLMEDKKRGLMVHEINNTVEFKGLIKVSKTNIPKEMI
                     EFALDSVRK"
     gene            complement(5298..5465)
                     /gene="lysW/argW"
                     /locus_tag="QGF01_06125"
     CDS             complement(5298..5465)
                     /gene="lysW/argW"
                     /locus_tag="QGF01_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HJN20494.1"
                     /translation="MNCPECDAELNIPNDAAVGEIVSCSDCGADFEISKKDGSTVEIK
                     QAETVGEDWGE"
     gene            complement(5475..6662)
                     /locus_tag="QGF01_06130"
     CDS             complement(5475..6662)
                     /locus_tag="QGF01_06130"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HJN20495.1"
                     /translation="MKQKAILAFSGGLDTSVVVKYLQVEHNMDVITVTVDVGQEDDYQ
                     QISKKAKKLGVVKHYNIDAKKEFVTDYIFPSIKANGLYQKKYCLATALARPLIAQKVL
                     QIAKKEKVTALAHGCSGKGNDQVRFDLTMRAGSNLPIIAPIRDLNLDRTTELKFAKKH
                     GIKIDSVAKKFSIDQNLWGRAIEGGVLENPINEPPDDAFIWIKTKNLPNKPAYMKIKF
                     EKGIPVAIDGKSMGPVKLIEYANKKAGSHGVGIVDHIEDRVVGIKSREVYETPAALCL
                     IEAHSDLEKMVHTKHQTKFKSLVDDEWSWLTYSGLWEDPLRKDLDMFIQQTQKAVSGT
                     VVLKLFKGSIRVVGRESKNSLYSHKIATYGKGSKFDQKLAKGFVELWGIQSTEANKLH
                     KKS"
     gene            6782..>7328
                     /locus_tag="QGF01_06135"
     CDS             6782..>7328
                     /locus_tag="QGF01_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJN20496.1"
                     /translation="MKILGMAVGGIVVIVAIAITITFFSNSDQINEDKIRVAFFPSIG
                     HIIPIVGLEEKIFEKGIGEEKQIETKLFDSGPQVIESIFSGSIDVAYVGPGPIINGFL
                     KSDGKDIKILSGAASGGASFIIQPNSGLESLENFDGKRIASPQISNSQDVSLRYYLES
                     HGLKSVEKGGTVFVLNISNPDI"
BASE COUNT         2162 a         1472 c         1064 g         2630 t
ORIGIN      
        1 ctaatttttt gagatacata cataccaatg aagaagagtg ctgcagcaag tatgagatgt
       61 gtggtatttt ttgtactact accaagcaaa acaaaagaaa tggttcctgg aataatacca
      121 atactggttg ctacaataaa attaattaat cgaacgtttg cagcaccagc atcacatgcg
      181 gcaaatgcat ttgcaagtgc aaacccaata tcattgtgaa catgtgcatc taatggtaca
      241 ttcacttcag aacgaactgt tttaacaaaa ttagacattc ctgtaggtcg taagactcca
      301 acggtatcag gaagactaat cctatccaca ccagcttctt ctattgcttt acatacctta
      361 atgaggaact gtggatcagc cctacatcca tcttctaccg tgaaccttat tttcagacca
      421 tgagatttag catattcaac agtttttact gccctatcca acgcttcttc tctagaaatt
      481 cttaacttat cttttagatg tatatcagaa attccaaggt atgcagcaac ccaagtcgca
      541 tcacatttta atgaaacatc aatatcttct tttaacgctc taccatgcgc aacaatatca
      601 gcacttaatc cctgtttgat gatcgtttta gttgcctctg catgatcatt agaaatgata
      661 ggagaaatct caatttgatc aaccccaaaa taatccaaca tccatgcaat ctgtattctt
      721 tgcttgattg tgaaagaaac tccagggtgt tgttcacctt ctctgagtgt agaatctaat
      781 attcttattc gctttggttc tttctctaca gcattgtatt cctctgcata ttgattcgga
      841 tcgttcataa ctatattcgt tataattata gattaatata aagataatcg tactaaaatc
      901 ggcatgatta gataatactt gtactgaatt aagtaatata tgtaaaaaat atacgatttt
      961 cgattatcgg atagttctaa gaattataac tgtatttgta tcaatcacac caggaatttt
     1021 tcttagatca tcaatacatg cgttgatctc agtaatattt ggagccttaa ttatcacact
     1081 gatatcatac tgaccagtaa tttcatagac agtttttact gcattcagtt ttgtcaattt
     1141 taccgaaacc ttcgatgtat caattgatga atctactgat accaaaacaa ttgcactagc
     1201 attctgttct tctatcgtaa acttttcaat gacaccattg ttgaccatat tttttactct
     1261 tcttctaacg gcagattctg atagttttag ttttttgcca ataacaacaa atgattctct
     1321 tgaatcatca cgtaaaaaac ctaaaatttt ttcatcggtg ttatccctat acattttttc
     1381 taccctcttc atttgttagt aatttatcta atatttgcag tgtatttgta acatcctcat
     1441 ctgagataac caaaggagga agaaatcgta gaatgtttct gccagaatat aatagcaaaa
     1501 gtccattttt gatcccttct aataatatat ccttcacttc aaattttagt tcaacaccaa
     1561 tcatgagacc tttccctctc acttccctga taatactatg tttacttttc atctcagtta
     1621 atccttgaaa tagtttttca cccatcatca ctgcattctt tagtagattg tcttgtgtaa
     1681 gcgcttgtat agttgcagtg ccagcagcac atgatacggg attaccacca aatgttgatg
     1741 agtgttctcc ttttttcata acagataaga tgtctgattt tactaatgta gcacccatag
     1801 gaatccctcc agcaattcct tttgctaaac acattatgtc tggtgttgtt tcccaatgtt
     1861 ctgatgccca cattcgtccc gttctaccca atccagattg gatttcgtca aatataagga
     1921 ggatctcgtt ttcatcacaa atctttctaa caccttgtaa gaatccatca ggtggaacat
     1981 gaattccact ttctccttga attggttcta agattatgaa agcagtatct tcatctacac
     2041 tgtctctcaa cgcatctaga tccccatatg ctgaaaattt tgcaccctca acaagcggtt
     2101 tgaatgattc tctgtatttc ggattaaatg taagagataa cgctcccatt gattttccat
     2161 ggtaagaacc tttcatagca atcatacctt ttttactcgt aaattttctg gcaaatttta
     2221 tagctgcctc tactgactct gcaccactat tgtttagatg aacctgtgtc aatccttttg
     2281 gcgcaatttt gaataaattt tctaaaaact cttccctagt tttactgtac aaagaactat
     2341 gaactgtaat tattttttca atttgagatt ttattgcatt tacaacacgt tcatttctat
     2401 gtccgacaag tgcaacacca taccctccca tacaatcaat gtattcttta ccgtttgaat
     2461 cccatacatg tgagcctaat cctttttcaa taacaacagg aaatctctgg taaagatttc
     2521 ctaaatattg atcttcactc attttttatc acggtacaag tatgatgttg aatcgccgag
     2581 gttatggggt tttccctttg cccatttgca atcaatgcct cttttacacc catttctagt
     2641 gcttcagttg cagcaagtat ttttttctcc ataccaaaac caatttgagg caaaatttct
     2701 ttcgctttcg ctagtgttag agtatctacc aatttctcat ccattaataa tccatcaacg
     2761 tttgtaagaa acaaaacctt atcagatttt acatgacctg caacatttgc agccgctcta
     2821 tcaccatcga cattaagaaa atcattttct tcactaattg ctattggaga gatgacaggt
     2881 gtataacctt gattaagaat tgttgttagc aattgcgcat taatctgtgt gattttacct
     2941 gtgtaaccgc catcaattgc ttgttttctt cctttttcat tcataattat gagttttttc
     3001 tttctgttgg cttgaattat ttttccatct attcccgata aaccaattgc attgattcca
     3061 tttttttgta acatccttac aattgtttta ttgattcttc cagacattac catagtgaaa
     3121 atctctgcag tttccaggtc agtatatctg ctctttacac caccagggga aacaacaaat
     3181 tttggttcct tacctaattg ttctgtaact tttgtaactt cctttcctcc accatgaact
     3241 agtataacac cctcttgttc tactactttt tttatgtcag ctacgactga agggtgcaga
     3301 tcatcaacta cactaccccc aattttgatt gtaatcattt actatactgg tgttaatggg
     3361 gaatacataa ttccttgctt ctcatcaaag cctgacatta cgttcatgtt ttgaatagca
     3421 gatcctgcag cacccttcat caaattatca gatgcagaaa gtgcaatcaa tctgttatta
     3481 tcttcgtcaa gatcaaaacc aatatcacaa aagtttgaac cgaccacaaa ctttggatca
     3541 gggaatttgt acaaaccttt tttatcgcga ataagtctga tgaattgctc ttcaccaaat
     3601 tgagcacgat aaattttcca aagatccttt tcactaactt cctttttcaa gaatgtgtga
     3661 tttgtacaaa gaattcctcg tactatatca actgcatgtg gactcattga gactttgatt
     3721 ttttttccag caacttcact aagctcttgt tctatttcac cagtatgacg gtgttttgca
     3781 ggtttgtaag gtcgaattac tccagctctc attgcatgtt gagtaccaga tgttcctcca
     3841 gcaccagcac cagacgaacc aatttttgaa tcaacaatta tgtggtctat atcaatgaga
     3901 tcatttttga tcagtgggta taatgcaagt gtagatgtta ccgccataca tccaggacaa
     3961 gaaactaatt gtgcattttt aattttatct ctatgaagtt ctggaacacc aaatactgat
     4021 ttttccaagt aatcaggatg tggatgttcc caaccatacc attttgtata gtcttcagga
     4081 ttatgtagcc tgtaatctgc actaagatca ataattttca ttcctctatc ataaaatgcc
     4141 tttacaatat cagttgcagt tccatgtggg actgctgtga aaactagatc acatttatcc
     4201 gacattttat cataatcaag ctctgaaaat gtcagttcag taaatccctt taggcttgga
     4261 tgtattctat gtagatattc tccaacatgc ttcgttgatg tgaccataga aacctcagct
     4321 tcagggtgac tcaacagtag acggactact tctccgccaa catatccaga tgctcctact
     4381 attcctactt tcattgtcat actcttcgca tttgttataa tttattgtta ttaatcggtt
     4441 tactttctaa ccgagtctag tgcaaactcg atcatttctt ttggtatgtt cgtttttgaa
     4501 actttgatta gtcctttaaa ttcaacagta ttattaatct catggaccat taatcctctt
     4561 ttcttatctt ccatcaaatc tattcctaat atacctccgc ctactgcttt tgatgccttc
     4621 gcacatagat cttctatctc atttgtgata tcacatattt caggatctgc acctagtgca
     4681 acattagtct taaatccacc agaagatttt ctatacattg cagcaattgg cctatcattt
     4741 acagttataa cacgaatatc tcttggtggt ctatcaacaa cttcttgaag atagaaaatt
     4801 ctatctaatg gaccatctgt aattcctctt tgttcaatta tcgcatcagc tgtgtcccta
     4861 tcttttaatg gtatcacact tcgtccccaa cttcctataa ctggttttat tattaatggg
     4921 taaccaattt tttcaatgtt ttctaatgca gcatcagcgg aaaaagaaaa gtatgtttta
     4981 ggtgttggaa tattattctt ttttaatatg attgatgtta acatcttatt tccacataaa
     5041 cttgaaacag aaaatttatt gattatagga acgtccaaaa attcaataca agcagtgaaa
     5101 tgaagtcccc tgtaatagct tatacaacgt tctaaaacca catcgccaaa atctgtattt
     5161 tgtttattac tatctgtgtc aaaatgagtt atttttgcat caaccatctt ggtttcattc
     5221 ccaagatcaa tagcttgttt ttcaagaagc ttttcttctg acctaagacg gtcaaataca
     5281 atacaaattt ttgacaatca ctctccccaa tcttcgccta ctgtctctgc ttgtttaatt
     5341 tctactgtgg aaccatcttt cttggaaatc tcaaagtcag ctccacaatc actacatgaa
     5401 acaatctcac caacagcggc atcattaggg atattcaatt ctgcgtcaca ttctgggcaa
     5461 ttcattattt tttttcagga ttttttatgt aatttattag cttctgttga ttgaattccc
     5521 cacaattcta caaacccttt ggctagtttt tgatcaaact ttgatccttt tccatatgtg
     5581 gcaattttgt gactatatag ggaatttttc gattcacgtc caacaacacg aatactacct
     5641 ttgaataatt ttaacacaac cgtcccagaa actgctttct gtgtttgttg tataaacata
     5701 tcaagatctt ttcttaatgg atcttcccat agaccagagt atgttaacca tgaccattca
     5761 tcatctacca atgatttgaa tttagtttga tgttttgtat ggaccatttt ttccagatct
     5821 gaatgagctt caatcaagca taaggcagca ggtgtttcat acacttctcg tgatttgatc
     5881 cctaccacac gatcctcaat atgatcaacg ataccaactc catgagaacc tgctttttta
     5941 ttagcatatt cgattaattt tactgggccc atagattttc catctattgc aacaggaatt
     6001 cctttttcaa atttaatttt catgtaagca ggtttatttg gaagattctt tgttttaatc
     6061 caaataaatg catcatcagg aggttcattg ataggatttt ctaatactcc accttcaatc
     6121 gcacgacccc ataagttctg atcaatacta aatttttttg caacactatc aatttttatt
     6181 ccatgttttt ttgcaaattt taattcagtt gttctatcta aattaagatc ccttataggt
     6241 gcaattattg gtagattaga acccgcacgc atagttaaat caaatctaac ttgatcatta
     6301 cctttacctg agcatccatg tgcaagtgca gtcacttttt ctttttttgc aatttgtaaa
     6361 accttttgtg caatcaatgg gcgagcaagt gcagttgcta gacaatattt tttttggtat
     6421 aacccattcg ctttaattga aggaaaaata taatccgtca caaattcttt ttttgcatca
     6481 atattgtaat gttttacaac tccaagtttt tttgcttttt tagaaatttg ttgataatca
     6541 tcttcttgac caacatctac tgtgaccgtt ataacatcca tgttatgttc cacttgaaga
     6601 tactttacaa caacagaagt gtcaagacct cctgaaaatg caagtatagc tttttgtttc
     6661 ataagggaat tgagtaatat gttttgcatt tatctcttat ctttcgttat attttacaaa
     6721 ttaccgcata gctcgagcta aaatccgctt actatttaac cctgaattat aacgctgttt
     6781 tatgaaaatc ttaggcatgg cagtgggtgg tattgtagta attgtagcta ttgctataac
     6841 tataactttt ttcagtaatt ctgatcaaat caatgaggat aaaattagag ttgcattttt
     6901 tcccagtatt ggacatatca taccaatagt tggattagaa gaaaaaattt ttgaaaaagg
     6961 aattggtgaa gagaaacaaa ttgaaacaaa attatttgat agtggtccac aagtaataga
     7021 atcaatcttt tcaggttcaa tcgatgttgc ttatgttgga ccaggtccaa tcattaatgg
     7081 ttttttaaaa tctgatggta aagacattaa aattttatca ggtgctgcca gtggtggagc
     7141 aagttttatc atccaaccaa attctggatt agaatcactt gaaaattttg atggaaaacg
     7201 tatcgcaagt ccacaaataa gtaatagtca ggatgtatct cttcggtatt atctagagtc
     7261 acatggtctc aaatcagttg aaaagggagg aacagttttt gttttgaata tttcaaatcc
     7321 tgatatct
//
LOCUS       DASCPJ010000129        15035 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001995544, whole genome shotgun sequence.
ACCESSION   DASCPJ010000129 DASCPJ010000000
VERSION     DASCPJ010000129.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001995544"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /gene="radA"
                     /locus_tag="QGF01_06140"
     CDS             complement(<1..1135)
                     /gene="radA"
                     /locus_tag="QGF01_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HJN20497.1"
                     /translation="MIDDLRLDSLPGVGPVTTRKLSDAGVHNIMDLIVRGPVELAEIT
                     GMDKDTSATIVEKARQALVEGGMISKDFVSASEIYKRRQEIGKITTGTECLDFLLDGG
                     LETQALTEVYGEFGCGKTQFCHTMCVTVQKSVEEGGLGGGVLYIDSENTFRPERIVTI
                     AKAHGMDPEKVLDNITVARAYNSAHQTLILEEASTIIRENNVKLLVADSAVGLFRSEY
                     LGRGTLAERQQKLNKFVHLLVRIAETYNVAALATNQVMSSPDTFFGDPTRPVGGNVVA
                     HTSTYRIYFKKSGKKRIARMVDSPHHPEQEVIFALGEIGVMDPDSDTKKKPTKTTKAP
                     KTEDVPKTVTEDTPKTVTEDTPKTVTEDTPKTVTEDTPKTVTED"
     gene            complement(1215..1397)
                     /locus_tag="QGF01_06145"
     CDS             complement(1215..1397)
                     /locus_tag="QGF01_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S30e"
                     /protein_id="HJN20498.1"
                     /translation="MPTHGSLTKAGKVRGQTPKVEGRKRVGTTSSLRNKSNFKKRFIL
                     QRFPGQNKPGQRRKRR"
     gene            complement(1443..3170)
                     /locus_tag="QGF01_06150"
     CDS             complement(1443..3170)
                     /locus_tag="QGF01_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJN20499.1"
                     /translation="MASPKIVLTADRTLMSHYRGLSLATFFGCAPALNPTRPHGSILY
                     KILGNQVTPKILFDFICNSSPHNNGVAKFAPYGLRKVEAGLLRDGFKREDVVVAHPDH
                     IEKFIGPETEVVGTHEMDPLGMGPVTMTFTYGRRQMSYDEFYCRDLHRRINAAKKKNG
                     SNAKVIAGGSGTWQYNYAPEKIGEYGLYAILEGELGGIAPEIDGHAGNFFNYLIDGQF
                     ENMDPFRKRKDFKVNVKEFKRTDKTHHGRFVNFWDRPDLDDIPNIVEPSMHGMIEVMR
                     GCGRGCKFCDVTLRSLRYYSPEKVKKEIEVNIKKGGIDRAWIHSDDIFVYGLDPTTTK
                     NMEPNREALEELFTAIMSTGVKHTNPTHGTLAGAIADERLIPNLSKITNAGPDNMTGI
                     QCGFETGSIRLIEKYADRKLSPYQPEEWHWVVKESVKTLNENYWIPAFTLIMGLDNDE
                     TPEDGWETIRLISELEQEQPDSMFTATPLTFVPIGLLEKSEFYDIGSGNDPTQLGVMY
                     KTWQHNFKYGIKKFMTKTGQKGSVRRTAFNGLARTLGGIPLGAMEKFARKRGPKFERV
                     IEKIKTNYW"
     gene            complement(3223..4035)
                     /locus_tag="QGF01_06155"
     CDS             complement(3223..4035)
                     /locus_tag="QGF01_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HJN20500.1"
                     /translation="MLRNPNIHHMCTIEKIIDETPTVRTLIFSDEILSNVLPGQFAMV
                     WIPGVNEIPMSVMVTQEKGKAAFTVRKRGESSTAMYNLKVGDYIGIRGPYGNHFDIKD
                     GRLLLVGGGTGLVPLLRLITFCKPFHNVTLLIGSQTKEEVFFEDLANKLLEKNEHRVI
                     PVTEDGTYGKKGFVTDVVEELLDENSFDAIYTCGPEIMMYKTVNLAISNQITVQASVE
                     RMMKCGVGICGSCCINEDLACRDGTIFEGTHLIQSDEFGQFHRTKSGKLEKI"
     gene            complement(4019..4930)
                     /locus_tag="QGF01_06160"
     CDS             complement(4019..4930)
                     /locus_tag="QGF01_06160"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HJN20501.1"
                     /translation="MKPSLSTSIGTLTLERPTILASGILGISLDIFERLYRSGAGAVV
                     TKSLSKEPWEGYVNPTIFSVKGGGWLNAVGLANPGATEFSNMISKSHDVPIIVSLVGS
                     TEDDFDFMIKQFENCKISAYELNLSCPHVAKVGLEVGDDVELVKKIVKKVKLNTNVPV
                     IAKVGLGTTNYLDTVGAACEAGADGITAINTIRAMAIDVETQKPILSNKIGGLSGTPI
                     KPIAVRCVYEISSKFDVPIIGCGGISTWQDAVEFILAGASAVQFGSVLGEHWTEVFAE
                     INNGIKNYMEKNGYSKMSEMIGNAKKS"
     gene            5019..6245
                     /locus_tag="QGF01_06165"
     CDS             5019..6245
                     /locus_tag="QGF01_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HJN20502.1"
                     /translation="MFRVILTELGIAVFDDKTRIKTFPFSEPADEYVSIKKGKSEINE
                     LSKFLVEKRKDVLVNDLALNDLLKKKDIETQIMSESEMEDIQLTKPKVLVNAEFAKDE
                     NDSIQKLREFAINLSSSKITEVSSSPDLHIIQAINTLDDTDKIINGISSRLREWYGLH
                     FPELDNIIDSVNGYAQIVMSGKRENMADSVFIDAGFPDSKVQMLSLVKEKSRGGDISE
                     ENLAIVQILAKNILELFDMRKSLEMQIDEQMKHVAPNISAVLGTTVGARILAKAGSLN
                     RLSTMPASTIQVLGAEKALFRSLKTGSNPPKHGLLFQHAIVHAAPRWQRGKIARAVAS
                     KASIAARVDVHGVGLNQTLLDKLNVRVKEIETKYSEPVDRPPRQNFDKNNKSKQRRKP
                     DRFKNRKRRNFGRRQR"
     gene            6226..6900
                     /locus_tag="QGF01_06170"
     CDS             6226..6900
                     /locus_tag="QGF01_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013335441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HJN20503.1"
                     /translation="MEDVKDNVFWFTIDGEKRLATKNLVPGKQVYNEKFVLSKGVEYR
                     TWDPFRSKLAASIMNDLEYFPFKEKSNVLYLGVSTGTTISHISDIVGPNGTIFGVEHS
                     SRVARDFLDRVACYRKNIIPIVQNARQPKEYFSIYNKVDVVYVDIAQPDQTNIAIENC
                     KMYLKPKGYLFLTIKARSIDVTKEPKHIISNEIKKLNLDFEIKQSIDLKPYDKDHAMI
                     IATSLN"
     gene            complement(6897..7520)
                     /gene="rnhB"
                     /locus_tag="QGF01_06175"
     CDS             complement(6897..7520)
                     /gene="rnhB"
                     /locus_tag="QGF01_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HJN20504.1"
                     /translation="MQICGIDDAGRGSMIGPLVIAGISIEKKNIKKLKSIGVRDSKKL
                     APKTREILYKKIIEIVDDYHIIKIPPKTIDRYVSNHNLNHLEAETMAKIVLNLHPDTS
                     YVDSCDVDYKRFGKEISALSSNAKIKSSHHADSRFIVVAAASILAKVNRDKTIHRLNK
                     KFNLGSGYPSDKKTVKSVKKILNSYKKPQPFIRTSWKPVQKIINTSN"
     gene            7539..8726
                     /locus_tag="QGF01_06180"
     CDS             7539..8726
                     /locus_tag="QGF01_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N1)-methyltransferase"
                     /protein_id="HJN20505.1"
                     /translation="MVLTVIGKKNSRKLLQGIKEKFVEITEGSTKILVLDKSISDKVP
                     PKKPVFFNPAAKLNRDFSILAYSAFWNDFDKPKIFLDGLAGVGARSLRVANEIKDVEK
                     VVINDVNSDALDVAQRSARLNNISNFEISENETCRFFSLFSQKDSRGSVVDIDPFGSP
                     TKYFDCSIRATMHGGLLSVTATDMQVLHGLAKNACKRKYYGVPIKTEYSNEIAIRLIL
                     GCLYAVSGRLGIQIIPQFVQTHMHYYRIFVKVLTKKDQNQNLGYISHCFECGNRMVEK
                     KSSNCCDKCNGELNSAGPLWIGQLFDKKFVNSMLNEIPKFIVDKKCVGILEKSVSEAE
                     MPATYYTLDKIASKIKGAPIKMKNAIKILQDAGYLASPTSLNPTGFRTNCTIDEVVKL
                     FEN"
     gene            complement(8723..9316)
                     /locus_tag="QGF01_06185"
     CDS             complement(8723..9316)
                     /locus_tag="QGF01_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HJN20506.1"
                     /translation="MKLIDARKDHYRKLAHEEGYRSRSSYKLKELNKSYRIIGPGYFV
                     LDLGCAPGGWTQVATQLVGNKGKVMGVDTSFVEEIPGVHILKASVDSDYIAEEVFSFF
                     GKKVNAVICDLSPQVTGNWAIDHSRQISLNYAAEKIMEKVLEKKGNALFKVFDGEYSN
                     EFYQFMKKKFSKSKLTKPKASRKPSSELYCTCLGYSN"
     gene            complement(9319..10155)
                     /locus_tag="QGF01_06190"
     CDS             complement(9319..10155)
                     /locus_tag="QGF01_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJN20507.1"
                     /translation="MVKKEITSTDELKKELEESKAKLALLNKKSSHPVKTKQKKKQAK
                     KKTEVKKLETKKTEANKLKTRKTEAKKKAEAKKKAEAKKKEAKKKAEAKKKEEAKKKV
                     EAKKKAEAKKKEEAKKKEEAKKKEEEAKKKEEEAKKTYEEELEEQLTEEEITGFQIDK
                     TDMDRLSNKVCEIVAGYETEGMFQSELWKKLKLSSRDGSRLALKLERMGMISREKILE
                     NGRWTYKLIIKRTPVSTSSLEGAPCLICPVEAQCSIEGEISPKTCQYIEDWVRIESKH
                     SN"
     gene            10269..12344
                     /locus_tag="QGF01_06195"
     CDS             10269..12344
                     /locus_tag="QGF01_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN20508.1"
                     /translation="MRLRRFRIRAFRCIHDSGDVTVGDLAAFVGRNESGKTTILQGLI
                     LLNRDEIVSELDLCDEMVEELKSEINVAEGVFELNEHEREFVKEKFPSLDIKRLKIFR
                     TNKNPEIQYDFEDGKIGEEQNTNLRSWQSITSDLQNFINTIPNHIQEQLDTDFFQLSS
                     PPKTIGDFKKDLQIFDESIKSVASEEEEVVSEWNELYPKILENSNGFLNTSERLELEE
                     FIKDNLHPRIVYFSDYKKILGNINLQDYLHGVKSSTSGNVEYVEEFDRVETVRNLFYL
                     AELELDKLEEYQNSPSKLIKLLNQASKKLTSRLNPAWKGEPIHVELRFNPGNIMSVII
                     SDVHRDGTITNTGLLNRRAEGFKWTFSFIINFAAETQRSELKEAILLLDEPARNLHPT
                     QQRGISDLLKNLAGTNQVLYATHSPFMIFDYTPGNLLVVELERKKHLSKIYYEYWKAD
                     DATLIPILYGLSRGLVESTVDREIGSNSRPIIIVETMSDTMYLNAFDKFLQDPNISMN
                     PLNVIPAYNKNSVLPLAIFYRDHGYNTFILLDNDNESKQIAEQLKNNKFSEVQTIFFE
                     REGKLLQSIEDYMVTEDYLYAVNQTYEIKLRKQGFENLTSNQVMVQGKKGVVENLTAV
                     WDEHREDEWGEFEKEEICRYVCEKIALGDARFLTEKTKDQVRTLYRLIAERIRQHQNS
                     LSNRRIIKK"
     gene            12400..14280
                     /locus_tag="QGF01_06200"
     CDS             12400..14280
                     /locus_tag="QGF01_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20509.1"
                     /translation="MTEKNMNPMKNYSKVNFIIILLAVVSLLVVFPVSEITAAETISV
                     NAKNLDNTIIIEFSNSPNNTSEIKTVKVWLGGDKTFKSFKTESGWGGGTYSDGKLLVF
                     TAVDSLKSGESVKFGLITNVKVTGINWKVLDKNDNAIDTNKTPIQEISQDLPNIPTSE
                     SETIQTSGDKIKFIPEKIRIASSERLIGNGFNPNEVLQLFLNDTLLESVRTDQNGNFI
                     TKLNIPATTKIGINDFQIKRQDGVYESTKLKINDSLNRFYETEHRFGITTIPETIKLE
                     DTLSISGTAKAQSGIILSFKNGNVLESKRVVVTDAAGKWNYDDIIGSDTLVGAKSVII
                     ENDQKSILKNITVTSDFVFQMISSSDRYDTGDTVTITGTAEANQELTLSIIDPKSVTI
                     LFDTIQADGSGKFEYKFPVTSTFTSGTYAVIAKTLNNNSEATIFGIGSAPTDKIIVLL
                     DRLNFQTTTDLTLRVVGPSSSTLNVRIIDSGDNIKVTDTLTTNSAGNGQITLDLNGYK
                     SGVYRAVVSYTSIEDIAKFSVGLQTGTGVITLSPTKSTYQPGEETVVIGQTANSNAIL
                     FLSLINPNGDKVSEIELFSDKNGQFTSNLLRIPLDAPPGVWTIEANSRLDTASATIDV
                     EI"
     gene            14336..14848
                     /locus_tag="QGF01_06205"
     CDS             14336..14848
                     /locus_tag="QGF01_06205"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HJN20510.1"
                     /translation="MNLKEIIADYQDFPKKGILFRDFSPILKNPAAMEFIIDEFSKNF
                     HPNDVDVFVGIESRGFIVACALAQKYNKGMVLLRKAGKLPGKTEKISYTIEYGKATME
                     IQKNALKEGERVVICDDLLATGGTAQAAAKLVEKMNAKNTGFAFIIELTDLNGIEKIS
                     KYKTVALVQY"
     gene            14848..>15035
                     /locus_tag="QGF01_06210"
     CDS             14848..>15035
                     /locus_tag="QGF01_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20511.1"
                     /translation="MTDQQIEIGIFGGTGIYDSGLLEDSKEIEYNASGGSEYFISYES
                     GTKLLAYVRLRLDDNATV"
BASE COUNT         4906 a         2543 c         2512 g         5074 t
ORIGIN      
        1 tatcctcggt tacagttttt ggtgtatcct cggttacagt ttttggtgta tcctcggtta
       61 cagtttttgg tgtatcctcg gttacagttt ttggtgtatc ctcggttaca gtttttggta
      121 catcttcagt ttttggtgct tttgtggttt ttgtaggttt tttcttcgta tctgaatcag
      181 gatccattac acctatttca cctaaagcaa aaataacttc ctgctctgga tgatgcggac
      241 tatctaccat tcgtgctatt cgttttttac ctgatttttt aaagtaaatt ctataagtac
      301 tggtgtgagc aaccacattt ccacctactg gacgtgtagg atcaccaaag aatgtatcag
      361 gagatgacat tacctggttg gtagctagag cagcaacatt gtatgtctct gcaattcgta
      421 ccaataaatg aacaaatttg tttaatttct gttgtctttc tgctaatgtt ccgcgtccta
      481 aatattctga tctgaataaa cctacagcag aatctgctac aagtaatttt acattatttt
      541 ctcggattat agtacttgct tcttctagaa tgagtgtttg atgtgcactg ttatatgctc
      601 tggctacagt gatattgtca agaacttttt ctgggtccat accatgtgct tttgcgatag
      661 ttacaattct ttctggtcta aatgtatttt cactgtcaat atacaatact cctcctccta
      721 atccaccttc ttctactgat ttttgaactg ttacacacat tgtatgacaa aattgtgttt
      781 ttccacaacc gaattcgcca taaacttcag ttagcgcttg tgtctcaagt ccaccatcaa
      841 gtaaaaagtc taagcactct gtacctgtgg ttattttacc aatctcttgt cttcttttgt
      901 atatttcaga tgcagagaca aaatctttag atatcattcc tccttcaaca agtgcttgtc
      961 ttgccttttc gacgattgtt gctgaagtgt ccttatccat gcccgtaatt tctgctaatt
     1021 ctactggacc acgaactatt agatccatta tattgtgaac acctgcatca ctaagttttc
     1081 ttgttgtaac tggtcctact cctggtaaac tatctaatct taaatcatct atcataccgt
     1141 aaggtacggt acgggtacgt tataaaggta ttccttgatt ttatttacac aagtattaag
     1201 atcgataaaa atgattaacg acgttttcgt ctttggccgg gcttattttg acctggaaat
     1261 ctttgcaaaa taaatcgttt cttaaaatta cttttgtttc taagactaga tgttgtgccc
     1321 acacgtttac gtccttctac cttaggtgtc tgacccctaa cttttcctgc ctttgtaaga
     1381 gaaccgtgag ttggcatgat attttctaaa attgttcaaa tttatgtatt tgtatatttt
     1441 gtctaccagt agtttgtctt gatcttttcg ataactcttt caaacttggg gcctcttttt
     1501 cttgcaaatt tttccattgc accgagtgga ataccaccca aagttctagc aagaccattg
     1561 aaagcagttc gtctaacaga gcctttctga ccagttttag tcataaattt cttgattccg
     1621 tatttgaaat tatgctgcca agtcttatac atcacaccta gttgtgttgg gtcattacca
     1681 ctgcctatat cataaaactc tgatttctca agcaaaccta tgggaacgaa tgtcaatgga
     1741 gtagctgtaa acatagaatc aggttgctct tgttcaagct cactgattag cctaatcgtt
     1801 tcccaaccgt cttccggcgt ttcatcatta tcaagaccca tgattaatgt gaatgcagga
     1861 atccaataat tctcgtttag ggtttttaca ctttccttaa caacccaatg ccattcttct
     1921 ggctggtatg gtgacaattt cctatcagcg tatttttcaa tcagcctaat gcttccagtc
     1981 tcaaatccac attgaattcc tgtcatattg tctggacctg cgttagttat ctttgataag
     2041 ttgggaatta atctctcgtc tgcaattgca ccagccagtg ttccatgagt tgggtttgta
     2101 tgtttaacac ccgtagacat aatcgcagta aataattctt ctaatgcttc acgatttggt
     2161 tccatatttt ttgtagttgt ggggtctaaa ccataaacaa aaatatcgtc actatgaatc
     2221 catgctctgt cgataccacc tttcttgata ttcacttcaa tttctttttt cactttttca
     2281 ggagaataat atcttaatga tcttaacgta acatcacaaa acttacagcc ccttccacaa
     2341 ccacgcatta cctctatcat accatgcata gatggttcaa caatatttgg aatgtcatct
     2401 agatctggtc tatcccaaaa gttgacaaat cttccatgat gtgttttatc agttcgttta
     2461 aattctttaa cgttaacttt gaagtccttt ctttttctga aaggatccat attttcaaat
     2521 tgaccatcga ttagatagtt aaagaaatta ccagcatgtc catctatttc gggagcaatt
     2581 ccacctagtt caccttctaa aattgcatat agaccatact ctccaatctt ttcaggtgca
     2641 taattatatt gccaagtacc agaaccacct gcaataactt ttgcattact tccatttttt
     2701 ttctttgcag cattgattcg tctgtgaagg tcacgacaat aaaactcatc ataagacatc
     2761 tgtcttcttc catatgtgaa cgtcatggtt accggtccca ttccaagcgg atccatttca
     2821 tgtgttccga caacctcagt ttcaggtcca atgaattttt caatgtgatc agggtgggca
     2881 actacaacat cctcacgttt gaagccatca cgaagtaatc cagcctctac cttgcgcaaa
     2941 ccatatggag caaattttgc tacgccatta ttatgtggtg aagaattaca gataaaatcg
     3001 aataaaattt ttggtgttac ttggtttcct aaaattttat acaaaatact accatgagga
     3061 cgagttggat taagtgcagg tgcacaacca aaaaatgtag ccaatgaaag tccacgataa
     3121 tgagacatca atgttcgatc cgcagttaat acgatttttg gacttgccat tactctaacg
     3181 taggacttaa atgatttatt ttaaaccttt tgagattcct gactatattt tttctaattt
     3241 tccagatttt gttcgatgaa attgtccgaa ttcatcactt tgtatcagat gggttccttc
     3301 aaaaatagtg ccgtcacggc atgccaagtc ttcattgatg caacaactcc cacatatgcc
     3361 aacaccacat ttcatcatac gttcaacgct tgcttggaca gtgatctggt ttgatatggc
     3421 cagatttaca gtcttataca tcattatctc cggtccgcaa gtatagattg catcaaaact
     3481 attctcatca agtaattctt ccacaacatc tgtaacaaat cctttttttc catatgtgcc
     3541 atcttctgta actggtataa cccgatgttc atttttttca aggagtttgt ttgctagatc
     3601 ttcaaagaat acttcttctt ttgtttgaga accaatcagc aaggtgacat tatggaaagg
     3661 tttacagaaa gtgatcaagc gtaataaagg aacaagacca gttcctcctc caactaataa
     3721 taatctgcca tctttgatat caaaatggtt tccatatgga ccacgaattc cgatataatc
     3781 acctacttta agattataca ttgcagttga tgattcacca cgttttctaa ctgtaaatgc
     3841 agctttacct ttttcttgag tgaccattac actcatgggt atttcattta cacctggaat
     3901 ccataccatt gcaaattgac ctggaagaac attagataat atttcatcag agaagattaa
     3961 tgttctaaca gttggtgttt cgtcaataat tttttcaatt gtgcacatat ggtgaatgtt
     4021 aggatttctt agcatttcca atcatttcac tcattttcga atatccattt ttctccatat
     4081 aatttttgat accattattt atttctgcaa aaacttctgt ccaatgctca cctaatacac
     4141 ttccaaactg cacagcactt gctccagcaa gaataaattc tacagcatct tgccatgtag
     4201 aaattccgcc acatccaatt attggaacat caaacttgga tgaaatttca taaacacatc
     4261 ttacagcaat tggcttaatt ggagttccag acaatccacc tatcttatta ctaagaatgg
     4321 gtttctgagt ttcaacatct atcgccattg cacgaattgt gttaattgca gtaattccat
     4381 ctgcaccagc ctcacatgct gcaccaacag tatcaagata gtttgtagtt cctagtccta
     4441 cttttgctat aacaggaacg tttgtattca attttacctt ttttacaatt ttttttacaa
     4501 gttcaacatc atcaccaacc tcaagaccaa ctttagcaac atgtggacat gacaaattca
     4561 attcataggc tgaaatctta caattttcaa attgttttat cataaaatca aaatcatcct
     4621 cggttgatcc aactaaactt acaattattg gaacatcatg actttttgaa atcatatttg
     4681 aaaattcggt agcacctgga tttgccaatc ccactgcgtt tagccaaccg ccacctttta
     4741 cactaaagat tgttggattt acataacctt cccatggctc tttgctcaat gattttgtga
     4801 caacagcacc tgcccctgaa cggtataaac gttcaaaaat atcaagtgag attcctaaaa
     4861 taccggaagc aagaatggtt ggcctctcca aagttaatgt tcctattgaa gttgagaggc
     4921 taggtttcac tctgaatagt ttaatagtat tctcatataa tagttgagta tcaaacacta
     4981 ttgttaactt ttaatgaggt gtaaacatta catttgttat gttcagggtt atcctaactg
     5041 aattaggaat tgcagttttt gatgataaaa cacgtatcaa aacattccct ttttctgagc
     5101 cagcggatga atatgtttct ataaaaaaag gtaaatcaga gattaatgaa ctgtccaagt
     5161 ttctagttga gaaacgaaaa gatgtacttg taaatgattt agcattaaat gacttgttaa
     5221 agaaaaaaga tattgaaaca cagattatgt cagaaagtga aatggaagat attcaattaa
     5281 ctaaaccaaa agttctggtt aatgccgagt ttgctaagga tgaaaatgat tctattcaaa
     5341 aactgagaga gtttgccata aatttatcat catctaaaat tacagaggtt tcaagtagtc
     5401 ctgatcttca tataattcaa gcaattaata ctttggacga cactgataaa ataattaatg
     5461 gaattagttc taggttaagg gaatggtatg gcttgcattt tcctgaactt gacaatatta
     5521 tagacagtgt aaatggctat gcacaaattg tcatgtctgg taaaagagaa aatatggccg
     5581 atagtgtgtt tattgatgca ggatttcctg attcaaaagt acaaatgcta tcattagtga
     5641 aagaaaaaag ccgtggtggg gatatttcag aagaaaatct tgcaattgtt cagatattag
     5701 ctaaaaatat cctagaatta tttgatatga gaaaaagtct agagatgcaa atagatgagc
     5761 aaatgaaaca cgtggcacct aatatttctg cagtacttgg tactacagtt ggtgcaagaa
     5821 ttttggcaaa ggcaggaagt ttaaacagat tatcaacaat gcccgcaagt acaattcaag
     5881 ttttaggagc tgaaaaggct ttgtttcgtt ctttaaaaac aggttcaaat cctccaaaac
     5941 atggattgtt gtttcaacat gcaatagtac atgcagcacc aagatggcaa cgaggtaaaa
     6001 ttgcaagagc tgtagcttca aaggcatcaa ttgctgctag agtagatgtg catggagtag
     6061 gtctgaacca aacacttctt gataaattaa atgttagagt aaaagaaatt gagacaaaat
     6121 atagtgaacc agttgacaga ccgccacgac aaaattttga taaaaataac aaatcaaagc
     6181 aaagaaggaa acctgataga tttaagaata gaaagcgtag gaattttgga agacgtcaaa
     6241 gataatgttt tttggttcac aattgatggt gaaaaaagac tagctacaaa aaatttagtt
     6301 ccaggaaaac aggtttacaa cgaaaaattc gttttatcaa aaggagttga gtatagaaca
     6361 tgggatccct tcagaagtaa attggctgca tcgataatga atgatttgga gtattttcca
     6421 ttcaaggaaa agtcaaatgt gctgtatctt ggagtttcta ctggaacaac aattagtcat
     6481 atatcagata tagttggacc gaatgggact atatttggtg tcgaacattc aagtagggtc
     6541 gcacgagatt ttttagacag agttgcatgt tataggaaaa acataattcc aatagttcaa
     6601 aatgctagac agccaaaaga atacttttca atttataata aagttgatgt agtttatgtt
     6661 gatattgcac aaccagatca aactaacatt gcaatagaaa attgtaaaat gtatttgaag
     6721 cctaaaggat atttattttt aacaataaaa gcaaggagta tagatgtaac taaagaacca
     6781 aaacacataa tcagtaacga aatcaaaaaa ttaaatttag attttgaaat taagcagtca
     6841 attgatctga agccatatga taaggatcat gcaatgataa ttgcaacatc attaaattag
     6901 ttactagtat tgataatttt ttgtactggt ttccaactag tacgaataaa tggttgtggt
     6961 tttttgtaag agtttaatat tttctttaca gatttgacag tttttttgtc agatggataa
     7021 ccgctaccaa gattaaattt tttattcaat cgatgaatag tcttatcacg attaactttt
     7081 gcaagtattg atgccgcagc aactacgatg aacctactat ctgcatgatg agacgattta
     7141 atttttgcat ttgaagatag cgcagatatt tctttaccaa atcgtttgta atcaacatca
     7201 caagaatcaa cataagaagt gtctgggtgt agattcaaaa ctattttagc cattgtttcc
     7261 gcttccaaat gattgaggtt atgatttgaa acataccgat ctattgtttt gggaggaatt
     7321 ttgattatat gataatcgtc gacgatttca attatcttct tatataaaat ttcacgtgtt
     7381 tttggagcaa gttttttaga atcacgaaca ccaattgatt ttagtttttt tatattcttt
     7441 ttctctattg atattccagc aattacaagt ggaccaatca ttgaacccct tccagcatca
     7501 tcaattccac aaatctgcat gagattttcc actttttgat ggtattaacc gttataggta
     7561 aaaagaattc aagaaaacta ttgcaaggaa taaaagaaaa atttgtagag ataacagaag
     7621 gtagtacaaa aattcttgtt cttgataaat ccatcagcga taaagttcct ccaaaaaaac
     7681 cagttttttt taatcctgca gcaaaattaa atagagattt ttcaatttta gcatattcag
     7741 cattctggaa tgattttgat aaaccaaaga tctttctaga tggactggcg ggcgttggtg
     7801 ctagatcact tagagtggca aatgaaataa aagatgttga aaaagttgta ataaatgatg
     7861 tgaattctga tgcgcttgat gtagctcaaa gatctgctag attgaataac atatcaaatt
     7921 ttgaaatctc tgaaaatgaa acttgtcgtt tttttagtct attttcacag aaagacagta
     7981 ggggatcagt tgtagatatt gatccatttg gctcaccaac aaaatatttt gattgttcta
     8041 ttagagctac aatgcatggt ggattactat cagtgactgc tacagatatg caggttttac
     8101 atggtctggc aaaaaatgct tgcaaaagaa aatactatgg ggttccaatt aaaacagaat
     8161 attcaaatga gatagcaata aggctaatac tgggttgtct ttatgctgta tctggtagat
     8221 tgggcataca gattattccg caatttgttc aaactcatat gcattactat agaatattcg
     8281 taaaagtact aacaaaaaaa gatcaaaatc aaaacttggg atatatttca cattgttttg
     8341 aatgtggtaa tagaatggtc gaaaaaaaat ctagtaattg ctgtgataaa tgtaatggag
     8401 aattaaattc tgcagggcca ctttggatag gacagttatt tgataaaaaa tttgtaaaca
     8461 gtatgttaaa tgaaattcca aaatttatag tggataaaaa atgtgtagga attttagaaa
     8521 aatctgtttc agaagctgaa atgcctgcta catattatac tctagataaa attgcttcta
     8581 aaattaaggg agcgccgata aaaatgaaaa atgccatcaa aatacttcaa gatgccggct
     8641 atttggctag tccaacttca ctaaatccta caggattcag aaccaattgt acaatagatg
     8701 aagttgtaaa gttattcgaa aactaattac tgtatcctag acacgtacaa tacaattcac
     8761 tgcttggttt tctgctagcc tttggttttg tgagtttaga tttagaaaat ttttttttca
     8821 tgaattgata aaattcattc gaatattcac cgtcaaaaac tttgaaaagt gcatttcctt
     8881 ttttttctaa aactttctcc ataatttttt ctgccgcata attcaatgag atttgtcttg
     8941 aatgatctat agcccaattt ccagttactt gaggggaaag atcacatatt acagcattta
     9001 cttttttccc aaaaaaggaa aaaacttctt ctgctatata atcactatca acacttgctt
     9061 ttagtatatg cactcctgga atttcttcta caaatgatgt atctactccc atcacttttc
     9121 ctttattccc aacaagttgt gttgcaacct gtgtccaacc acctggtgca catcctaaat
     9181 ctaaaacaaa ataacctggt ccaattattc gataagattt gttgagttct tttagtttgt
     9241 aagaagatct gcttctataa ccttcttcat gggctaattt tctataatga tctttccttg
     9301 catctattaa tttcattttt aattactatg cttactttct atacgaaccc aatcttcaat
     9361 atattgacag gtttttggac taatttcacc ttcaattgaa cattgtgctt ctactggaca
     9421 tatcaaacat ggtgctccct ctaaagaaga tgtgctaacc ggtgttcttt taattattaa
     9481 tttgtatgtc catcttccat tttctaatat tttttctcgt gaaatcattc ccattctttc
     9541 caactttaat gccaaacgtg aaccgtctct gctggacagc tttaattttt tccaaagctc
     9601 actttggaac attccttctg tttcataccc agctacaatt tcacaaacct tgttagataa
     9661 tctatccata tcagttttat ctatttggaa accggtgatc tcttcctcag taagttgctc
     9721 ctctaattct tcttcatatg ttttttttgc ttcttcttct ttcttttttg cctcttcttc
     9781 tttctttttt gcttcttctt tcttttttgc ttcttctttc ttttttgcct cagccttctt
     9841 ttttgcttcc actttctttt ttgcttcttc tttctttttt gcctcagcct tcttttttgc
     9901 ttctttcttt tttgcctcag ccttcttttt tgcctcagcc ttcttttttg cctctgtttt
     9961 cctagtcttc agtttattgg cttctgtttt cttggtctct agctttttca cttctgtctt
    10021 cttttttgct tgtttctttt tttgtttagt tttaactgga tgactagatt ttttgttgag
    10081 taatgctaat ttcgctttac tttcctcaag ttcctttttg agttcatcag tagatgttat
    10141 ttcttttttt accataaaat cactattatt ttctcagact ttcaaccctt attatctatt
    10201 ttgagagtta gtggttgaaa ttactttgac gaataagtta aatcgcagtc gaatcaactc
    10261 gctctagttt gcgtcttaga agattcagaa ttagagcttt tcgttgtatt cacgactctg
    10321 gcgatgtcac agtaggtgat ttagccgcat ttgtaggaag gaatgaaagt ggtaaaacaa
    10381 caatacttca aggactaatc ttactgaata gagatgaaat tgtatcagaa cttgatctat
    10441 gtgatgaaat ggttgaagaa ctcaaatctg aaataaatgt tgctgaagga gtatttgagt
    10501 taaatgagca tgaaagagaa tttgttaagg aaaagtttcc tagtttagat attaaaagat
    10561 taaaaatttt tagaaccaat aaaaatcctg aaattcagta tgattttgag gatggaaaaa
    10621 ttggtgagga gcaaaataca aatctgagat cttggcaaag tattacttca gatttacaaa
    10681 attttattaa cactatccca aatcatattc aagaacaact tgatactgat tttttccaac
    10741 ttagttctcc accaaaaact attggtgatt ttaaaaaaga tcttcaaata tttgatgaga
    10801 gtattaaatc tgtagcgtct gaagaagaag aagttgtttc tgaatggaat gaattgtatc
    10861 cgaagatact agaaaattca aatggttttt tgaatacttc agaaaggcta gaattagaag
    10921 aattcatcaa ggataattta catccacgta ttgtttactt ttctgattac aagaaaattc
    10981 ttggaaacat aaaccttcaa gattatcttc atggtgtaaa atcttctacc agcggaaatg
    11041 ttgaatatgt cgaagaattt gacagagttg aaactgtaag aaatttgttt tatcttgcag
    11101 aactggaatt ggataaactg gaagagtatc aaaatagtcc gtcaaaatta attaaacttc
    11161 taaatcaagc aagtaaaaaa cttacaagtc gcttaaatcc tgcttggaaa ggagaaccaa
    11221 ttcatgtgga attaagattt aaccctggaa atattatgag tgtgataata tctgatgttc
    11281 atagagatgg tacaattact aatacaggtt tgttaaatag aagagctgaa ggtttcaaat
    11341 ggacattttc atttattata aattttgcag ccgaaacgca gagatcagaa ttaaaagaag
    11401 caattttgtt acttgatgaa ccagctagaa atttacaccc aacacaacaa cgtggtatct
    11461 ctgatctact caagaatctt gcgggaacga atcaggtttt atatgctaca cattcaccgt
    11521 ttatgatttt tgattatact cctggtaatc ttctagtagt agaactagaa agaaagaaac
    11581 atctcagcaa aatttactat gaatattgga aggcagatga tgctactctc ataccaatcc
    11641 tatatggttt gtcaagagga ctagttgaat caacagtaga tagagaaatt ggtagtaatt
    11701 cacggcctat cattatagtt gaaactatgt cagatacaat gtacctaaat gcttttgata
    11761 agtttcttca ggatccaaac atatccatga atccgttaaa tgttatacca gcttataata
    11821 aaaattcagt attaccatta gcaatttttt atcgtgatca tggatataac accttcatcc
    11881 ttcttgacaa tgataacgag tctaaacaga ttgctgaaca attaaaaaat aataaatttt
    11941 cagaagtaca gaccatattt tttgaaaggg aaggaaaact tcttcagtca attgaagatt
    12001 atatggtgac tgaagattat ttgtatgctg taaatcagac ttatgaaata aaacttcgaa
    12061 aacagggatt cgaaaattta accagtaacc aagtgatggt ccaagggaaa aaaggtgttg
    12121 tcgaaaatct gacagcagtt tgggatgagc accgtgagga tgaatgggga gaatttgaaa
    12181 aagaggagat ttgtagatat gtctgtgaaa aaattgcttt gggagacgca agatttttga
    12241 ctgaaaagac gaaagaccag gttcgtacat tatatagatt aattgctgaa cgaatacgtc
    12301 aacatcaaaa ttccctgtct aatagacgaa ttataaaaaa atgaaagtta tttctagtct
    12361 aaaactgaac caaattagtc ataaagtaat taaagtttaa tgacagagaa aaatatgaat
    12421 cctatgaaaa attattccaa agtgaatttt ataatcatat tattagcagt tgtaagtctt
    12481 ttagtggttt ttccagtttc agaaattacg gcggcagaaa ctatttctgt taatgctaaa
    12541 aatctggata atacaataat catagagttt tcaaacagtc caaataatac ctcagaaata
    12601 aagacggtta aggtatggtt aggtggagat aaaacattca aatcattcaa gacagaatct
    12661 ggatggggag gaggaacata ttctgatggt aaactgctag tgtttactgc agtagatagt
    12721 ctaaagtctg gtgaatcagt aaaatttggt cttataacaa atgtaaaagt aactggaata
    12781 aattggaaag tacttgataa aaatgataat gccatagata caaacaagac tccgattcaa
    12841 gaaatcagtc aagatttgcc aaatattcct acatcagaat ctgaaactat tcaaaccagt
    12901 ggtgacaaaa ttaaattcat cccagaaaaa attagaattg cttctagtga aagattgata
    12961 ggtaatggat ttaatccaaa tgaagtatta caattattcc tgaatgatac tctattagaa
    13021 tctgttagga cagatcaaaa tggaaatttc ataactaaat taaatattcc ggctacaacc
    13081 aaaattggaa ttaatgactt tcaaataaaa agacaggatg gtgtatacga aagtactaaa
    13141 ttgaaaataa atgattcgtt aaatcgattt tatgaaactg aacataggtt tggtataaca
    13201 actatacctg aaactataaa attagaagat acattaagta tttcaggtac tgcaaaagca
    13261 caaagtggaa ttatactttc attcaaaaat ggaaatgttt tagaaagcaa acgggttgta
    13321 gtaactgatg ctgctggaaa atggaattat gatgacatta taggttcgga tacacttgtg
    13381 ggtgcaaaat ctgtaattat tgaaaatgac caaaaaagta tactaaaaaa cataacagtt
    13441 acatcagatt ttgtttttca aatgatttca tcatctgatc gatatgatac tggagatact
    13501 gtaaccataa ctggtactgc tgaagcaaat caagaactca ctttaagtat tatagatccc
    13561 aaatctgtta ctattttatt tgatactata caagcagatg gaagtggaaa atttgaatat
    13621 aaatttcccg ttacatccac atttacaagt ggaacatatg cagttatagc aaaaactctg
    13681 aataataact ctgaagctac aatttttggt attggttcag caccaacaga caaaattatt
    13741 gttttacttg atcgattaaa ttttcaaaca actactgact taactttacg agttgttgga
    13801 ccttcgtcgt caaccctaaa tgttcgaatt atcgattctg gagataatat caaggtaact
    13861 gatactctta ctacaaattc tgctggtaat ggtcaaatta ctttagattt gaatggatac
    13921 aagtcagggg tatacagagc tgtagtttca tatacaagta tagaagatat agctaaattt
    13981 tctgttggat tacaaaccgg aactggagta ataacattaa gtcctactaa atctacctat
    14041 caacctgggg aagaaactgt tgtgattggt caaactgcca atagtaatgc tatactattc
    14101 ctatctctaa ttaatccaaa tggtgacaaa gtatcagaaa tagaattatt ttctgacaaa
    14161 aatggacagt ttacttcaaa tttgcttcga atacctcttg atgcacctcc aggtgtatgg
    14221 acaatcgaag ctaacagtcg tttagataca gctagtgcta ctatagatgt tgaaatttag
    14281 atcatagtgt ttagtatcaa aacaattttt attcctcata tattcttgaa attatatgaa
    14341 cttgaaagaa ataattgcag actaccaaga ttttcctaaa aaaggtattc tctttaggga
    14401 ttttagtcca attcttaaaa atcctgctgc aatggaattt attattgatg aattttcaaa
    14461 aaactttcat cctaacgacg ttgatgtatt tgttggaata gaatcaagag ggtttattgt
    14521 tgcgtgtgct cttgctcaaa aatacaataa aggaatggtt ttgttaagaa aagctggtaa
    14581 acttccagga aaaactgaaa aaatttcata tacaatagaa tatggaaaag ccaccatgga
    14641 aattcaaaaa aatgcattga aggaaggaga acgtgtggtg atttgtgatg atttgttggc
    14701 tactggtgga acagcacaag cagctgcaaa attagtggag aaaatgaatg caaaaaacac
    14761 tggttttgca tttatcatag aactaactga tctaaatgga attgagaaga tcagtaaata
    14821 caaaactgtt gcactggtac aatactaatg actgatcagc aaatagagat cgggattttt
    14881 ggtggaacgg gaatttatga ttccggcttg ttagaagata gtaaagaaat tgaatacaac
    14941 gcaagtggag gaagtgaata tttcatatca tatgaatctg gaacaaaatt actagcatat
    15001 gttaggttaa gattagatga taatgccaca gttag
//
LOCUS       DASCPJ010000130        10932 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002004352, whole genome shotgun sequence.
ACCESSION   DASCPJ010000130 DASCPJ010000000
VERSION     DASCPJ010000130.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002004352"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1881
                     /locus_tag="QGF01_06215"
     CDS             <1..1881
                     /locus_tag="QGF01_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJN20512.1"
                     /translation="SSDWVKAIIVPDLETLTSLAHVVRSKNLPKLKIIPLNTIPELHF
                     KLPKTPGAIGILSDYIQCDEKYRPIARFLFGNVILTETGKDAYKLSNAGYKAVSLDGE
                     FFESKVSAMTIDIDSKISKLTKIISQSSTVEGLLETITLLRKHIQKKKHRLKKIEDKH
                     RHYMEQQKTSDTEAGTTSHSNNNLKSRINNTIKLNEDFSTRILQLKKQKEQLAPRIIQ
                     LTSSIESLNQRIKLVRQNYSDIAQNSVAEKLRLLNKEKAEYQTSQTRITNKLTEEEAS
                     ISVIVNEKKQKKRQFENERSSLSTEKDELKGSIIGLQEKKKTSENNLIVLRDEEQKLI
                     STSGTSASKLTDFDDTINQKKDTEDSITTRISKLERESDTLERDLLEIKGKELSLNKI
                     LRSFEFDGQIETFDVDPLIQSLDKEHSSLASSLNAIAPQRYVEISTGYRSMSGRKNDL
                     EEERNTIVSFIESIEKDKRQTFLDAFDTVDKEIRGIFSKMNGGNAWLELENEDDIFNS
                     GISYIIQFQNKPKRESTSISGGEKTLAAVVFVLALQKLKPSPFYLFDEIDAHLDAPNS
                     ERLANIVEERSNGSQFIMVSLKDSVVQKAKLIYGVYPERGVSKVVTYKDKRIPSISKI
                     AN"
     gene            complement(1878..2840)
                     /locus_tag="QGF01_06220"
     CDS             complement(1878..2840)
                     /locus_tag="QGF01_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJN20513.1"
                     /translation="MNEVLKVDNLSKTFTKKNFFGSKTSTVRAVEGVTFTLHDNETLV
                     IAGESGSGKTTIAKLILGAIRPDSGKIIFDGNEITDNDVSLRKIRMHCQMVHQDPYDS
                     INPRMRVIDIISEPLEIHNIGNDSERKKIVLDAIREVKLEPAEEILHKFPHMLSGGQR
                     QRVILARALVLRPKVIIADEPVSMLDVSIRAEILALMKQIQENNSISFIYITHDLVTA
                     KYFGQKMIILYLGKIMEEGIINEILANPKHPYTQALIDAIPEPDPDNLYKERKIRIND
                     PTNVDVYSGCRFRARCPYVIEKCKDVPPLLEKNDRRFACFVDIN"
     gene            2928..3956
                     /gene="egtD"
                     /locus_tag="QGF01_06225"
     CDS             2928..3956
                     /gene="egtD"
                     /locus_tag="QGF01_06225"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HJN20514.1"
                     /translation="MSLKEIQNNMEYQKVILDQRLRYYEPNGERVYKTFADEISNSLR
                     SNQKSINPKFFYDEKGSNLFEKICALPEYYLTNAEISILNQLDNKISSHLDGDFRLVE
                     LGSGSSTKTRILIQILESLQTYVEYIPIDISRILKTSSRILQSDFKRLNITGIIDEYE
                     SGLKFLKYYDTKKNLIIFLGSSFGNFDLEDGSKFLSEINSAMKKDDLFLIGLDLVKDK
                     KILECAYNDSEGITAQFNLNILSRINSELGGNFDLQKFRHHAVYNEDQNKIEMYLRSI
                     DRQTISIPQADLTLNLEKDELIHTENSQKFTISNIKQISEKAGFEIKDMWYDEKQYFA
                     MILLSKND"
     gene            complement(3953..5257)
                     /gene="egtB"
                     /locus_tag="QGF01_06230"
     CDS             complement(3953..5257)
                     /gene="egtB"
                     /locus_tag="QGF01_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HJN20515.1"
                     /translation="MATVSRQKSDLLEQFNETRIRTLNLVQTLEKDDFVVQTASFMSP
                     PKWHLGHVSWLFEIVMSKTSQNYEFYSEEFNEYLNSYYHQFGEPHDKDKRGLATRPTV
                     DQIFEYFHIITNNVSNILQNEVLDAKTQQLYLMAINHEYQHQELLVYDLQHLLADKYR
                     PVTHNTFPSTLQQEYNSVKIDGGLYPLGCSSDQFCYDIELPEHKVYLNDYKIDVYPIT
                     NRQYIEFIEDGGYDDFHYWLSDGWEKIENEGWNAPMYWEKVDGQWMTCDFLGRRKINP
                     NEPVCHVSFYEADAYCKWAGKRLPTEAEWEKAACWNEKSQSKSIFPWGNESPDIKHAN
                     LLESYLWNCTEIGAYPEGKSHYGCHQMIGDVWEWTCSEFTGYPGFKTGFSEYNDKWFA
                     NQKVLRGGSFGTPSSSIRSSYRNFFRLDERWLFSGFRCVEDV"
     gene            complement(5405..5716)
                     /locus_tag="QGF01_06235"
     CDS             complement(5405..5716)
                     /locus_tag="QGF01_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20516.1"
                     /translation="MGLNVRFTVGVIVIIVVGFSASAYFFSMNPFMDSINMMVIDKSQ
                     YIEKMDECLNKQTIGSITLNSFTAKWVADFKEKIMKAETTEELENTMNEFYTIVSHCK
                     P"
     gene            complement(5728..7170)
                     /gene="proS"
                     /locus_tag="QGF01_06240"
     CDS             complement(5728..7170)
                     /gene="proS"
                     /locus_tag="QGF01_06240"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HJN20517.1"
                     /translation="MNKEVGITASKNDDFSEWYTQVVIKAELADYAPVKGLIVLRPDG
                     YSIWESIKESLDKKLKETGHRNGFLPVLIPESLLGKEKKHFEGFNPEVFWVTQSGDSE
                     IGDRLALRPTSETLAYSLYSKWIKSWRDLPLKINFWNSALRAEITGTKPFLRTSEFLW
                     QEGHTVHATQDEAENEVMEILNIYKKTIEEELAIPVITGKKSEKDKFVGAVYTTTLES
                     LMPDGKALQMGTSHFLGQNFSKPFDVKYLDVNNVETYAWQASWGVSWRLIGGMIMVHG
                     DSKGLVLPPKVSPIQVVIIPIYYSKEDRETMILEANKIKDSLASNNVRVELDDRDNLT
                     PGFKFNDWELKGIPIRIEIGPKDIAKNQVVMARRYNQTKDDISLDKVSETINLELENI
                     QQQMFVNAKKILDERVVRVSEYQQFKEELKNGKMIDCSWCGNQTCEDKIKEETGAGLR
                     VVPFDGTQKSETCIYCKNSGTTNALFAKGY"
     gene            7249..7623
                     /locus_tag="QGF01_06245"
     CDS             7249..7623
                     /locus_tag="QGF01_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HJN20518.1"
                     /translation="MAKSTNWVKVVEKILSFDKKMRFVAIIDLKGNITEGIMKEGKSS
                     LESQRHQEHFCAQVALRRKMRKEFDKSLGAVNYVHIERKNVTQIVVYTRFKTIFVTIE
                     PNLPISRKLQIIKKIKQLAAKL"
     gene            complement(7637..7924)
                     /locus_tag="QGF01_06250"
     CDS             complement(7637..7924)
                     /locus_tag="QGF01_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20519.1"
                     /translation="MSNELDFKYEVEIKTSEKKQLLSKEVKGQLKESGMMDEKGKLKI
                     NGVSGKMLKRMKGEYVECPVLKETVQFIPCFICKNFQSRVTGKVLCKGDNL"
     gene            8041..8679
                     /gene="serB"
                     /locus_tag="QGF01_06255"
     CDS             8041..8679
                     /gene="serB"
                     /locus_tag="QGF01_06255"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HJN20520.1"
                     /translation="MLVIFDVEGVLYDAEYLPLLAEKMNKEKEVWEITKKGIQGKIDW
                     EEGLRERVDLLKGLDYKTCKDVADSMPIMTGARELCSALKNAGWKLMAVSGGFTIITD
                     RLKTELGIDHIFTNELIFNDEKLDDVVVNVNADKSKSAISKITEWNEKKENITVVVDG
                     ANDVKLFDISELGIAFRAQDLVKDLATVTLDEKDLTKIIPIINNHYNLTLSM"
     gene            8739..9464
                     /gene="cofE"
                     /locus_tag="QGF01_06260"
     CDS             8739..9464
                     /gene="cofE"
                     /locus_tag="QGF01_06260"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HJN20521.1"
                     /translation="MQIIPVKIQKDIVPNDSLVDLVLESTEIQDGDILVFSQKIVSKS
                     EGRILSLSSVNPSLLANGISSSYNKDPRLMELILSESKRIVRMENGIIIVETNHGFVC
                     ANAGIDESNVQDGYATLLPEDPDRSSNLLKERIEQKTGKNIAVIISDTFGRPFRLGQT
                     DVAIGVAGIEPILDYSGKPDTFAKIMHVTAIAIADEICSATELVMGKVEKCPVALVRN
                     YKFNFSDAKIQNLLRPKHEDLFR"
     gene            9502..10419
                     /locus_tag="QGF01_06265"
     CDS             9502..10419
                     /locus_tag="QGF01_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HJN20522.1"
                     /translation="MESLKTELHCHNIFSNGHVGVLEPIHDCSVTISQQLEQAHMEGL
                     DVLFVTNHNTIDGYEQMLEYKKNHWKFDTLNVYPAEEVTTDNESHVLVYGIDHAIKPG
                     LSLHEILDEAKKQNAVTIAPHPFSLMDALREDSVHCDLFEVFNSSNVDVYSNIKAKKF
                     SEEKNLNVVAGSDSHVKSTIGRCTNLIHSENKLDDIIHAMRHNQIKINTTGYVQPREV
                     LEHIRYKIENSKNYIDIHVSEFYPRSAWAFSLLYKLYMLSPEGVFCNALYRISISMLK
                     RISKKINFEGYNPSIFRERDLGTIAKMIF"
     gene            10476..10709
                     /locus_tag="QGF01_06270"
     CDS             10476..10709
                     /locus_tag="QGF01_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HJN20523.1"
                     /translation="MSESGKTLDARGLFCPSPVMQTNVELSKMQIGEILTILADDPAA
                     EDDIASWARRLGHEIVKMEKNGNEIHFEIKKVK"
     gene            10770..>10932
                     /locus_tag="QGF01_06275"
     CDS             10770..>10932
                     /locus_tag="QGF01_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012965.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HJN20524.1"
                     /translation="MTGQRIDGIEVSNAVKEILSRAVTHIKSEGLMPCLATVLIGDDP
                     ASATYVKNKH"
BASE COUNT         3757 a         1874 c         1768 g         3533 t
ORIGIN      
        1 agttctgatt gggttaaagc tataattgtc ccagatttag agacgttaac tagtcttgct
       61 catgtagtac gctcaaaaaa ccttccaaaa ttaaaaatta ttccacttaa cactatacct
      121 gaattacatt ttaaactacc aaaaactcct ggcgcaatag gcattctttc tgattatatt
      181 caatgtgatg agaaataccg cccaattgct agatttttgt ttggcaatgt tattttaaca
      241 gaaacaggaa aagatgcata caaattgtcc aatgcgggct acaaagctgt ttctttggat
      301 ggtgaatttt ttgaatccaa agtatctgct atgactattg atattgattc taaaatatct
      361 aaactaacaa aaattatctc acagagttca acagtggagg gcttattgga gacaattaca
      421 ctgctgagaa aacatatcca aaagaaaaaa caccgtctga aaaaaatcga ggataaacat
      481 cgccattaca tggaacagca aaaaacttca gatacagaag caggaaccac atcacacagt
      541 aataataatt taaaatcacg aataaataac acaattaaat taaacgaaga tttttctact
      601 agaattttac aactgaaaaa acaaaaggaa caactcgctc caagaattat tcaattaaca
      661 tcaagtattg agtctcttaa ccagagaata aaattagtaa gacagaatta ttctgatatt
      721 gcacaaaata gtgttgcaga aaaacttcgt ctgttaaata aagaaaaggc tgagtatcaa
      781 actagccaga ctagaataac gaataaacta actgaggaag aagccagtat atctgtgatc
      841 gtaaatgaaa agaaacaaaa aaagcgtcaa tttgagaatg agcgatcttc cctcagtact
      901 gagaaagatg aattgaaagg atcaatcatt ggtttacaag aaaagaaaaa gactagtgaa
      961 aataatctga tagtgttaag agatgaagag caaaaactga tttcgacctc aggaacttct
     1021 gcatctaaac taactgattt tgacgataca attaatcaaa aaaaggacac agaagatagt
     1081 attacaactc gtatcagtaa gcttgaaaga gaatctgata ctcttgaacg tgatctctta
     1141 gaaatcaaag gaaaggaatt atcactaaat aaaattctgc gttcatttga atttgatggg
     1201 cagattgaaa cctttgatgt ggatccatta atacaatctt tagacaaaga acatagctct
     1261 ctagccagct cattgaatgc catagcaccg caaagatatg ttgaaatatc tactggttat
     1321 cgttcaatgt caggcaggaa aaatgatctt gaagaagagc gcaatacgat tgtgtcattt
     1381 atagaatcca ttgagaagga taaaaggcag acattcttgg atgcttttga tactgttgac
     1441 aaggaaatca ggggtatttt tagtaaaatg aatggtggca acgcctggct cgaattagaa
     1501 aatgaagacg atatattcaa ttcaggaata tcttacataa tccagtttca aaacaagccc
     1561 aaaagggaat ccacttctat cagtggagga gaaaaaactc ttgctgccgt agtttttgtt
     1621 ttggctcttc aaaaattaaa accatctcct ttttacttat ttgatgaaat tgatgctcac
     1681 ttagatgcac caaactctga gagacttgca aacattgtgg aagaaagatc aaacggcagt
     1741 caattcatta tggtttcact aaaagattct gttgtgcaaa aagcaaaact gatctatggt
     1801 gtatatccag aacgtggtgt ttctaaagtt gtaacataca aggataaacg aattccttca
     1861 atctcaaaaa tagcaaacta gtttatatca acaaagcaag caaatcgtct atcatttttt
     1921 tccaataatg gtggcacatc cttgcatttt tcaattacat aaggacatct tgcacgaaat
     1981 ctacaaccag aataaacatc tacattagtt ggatcattaa ttctaatctt tctttcctta
     2041 tacaagttat ctggatcagg ttcaggtatt gcatcaatca atgcttgagt gtatggatgt
     2101 tttggatttg ccaaaatttc atttatgatc ccttcctcca taattttacc aaggtataat
     2161 atgatcatct tctgcccaaa atattttgca gtcactaaat catgtgttat gtaaataaac
     2221 gaaatagagt tattctcttg aatttgtttc ataagtgcaa gtatttcagc acgtattgaa
     2281 acgtctagca ttgaaacagg ttcatccgct atgattactt ttggtcgtaa tacaagtgct
     2341 ctagctaaaa taactcgttg tctttgtcca cctgataaca tatgaggaaa tttatgtaag
     2401 atttcttcag caggctcaag cttgacttca cgaattgcat ctagaacaat tttttttcgt
     2461 tctgaatcat ttccaatgtt atgaatttcc aatggctcag aaataatatc gattactctc
     2521 attctaggat taattgaatc ataaggatct tgatgtacca tctggcaatg catccgaatt
     2581 ttccttaaac tcacatcatt atctgtaatt tcgttaccat caaagattat tttaccagaa
     2641 tcaggtctta tcgctcctag tatcaatttg gctatagtcg ttttccctga tcctgattca
     2701 ccagcaatta caagtgtttc attatcatga agagtgaatg tgacaccctc aacagccctc
     2761 acagttgatg tttttgaacc aaaaaaattt tttttagtga atgtttttga taaattatct
     2821 accttcaaaa cttcattcat gagttattct gatttatcta ctatatcaag aatacaaatt
     2881 ttacacagaa ggaaaaagat ttaatcagaa caattttttt gcattttatg agtttaaaag
     2941 aaattcaaaa taatatggaa tatcaaaagg ttatccttga tcaaaggtta agatattacg
     3001 agccaaatgg agaacgggta tacaaaacat ttgctgatga aatatctaat agtttaagat
     3061 caaatcaaaa atcaatcaat cctaaatttt tctatgatga aaaaggctcc aatctttttg
     3121 aaaaaatttg tgcattgcca gaatactatc ttactaatgc tgaaattagc atattaaatc
     3181 aattagataa taaaatttca tcacatcttg atggagattt cagactagtt gaattaggca
     3241 gtggttcatc aacgaaaact agaatactta ttcaaattct tgaaagccta caaacatacg
     3301 tggagtatat cccgattgac atttctagaa ttcttaaaac cagttctaga atactgcaaa
     3361 gtgattttaa aagattgaat ataacaggaa ttattgatga gtatgaaagt ggtttaaaat
     3421 ttttaaaata ttatgatacg aagaaaaatt tgattatatt tttaggatct agttttggta
     3481 attttgattt agaagatggc tcaaaattct tatctgaaat aaactctgcc atgaaaaaag
     3541 atgatctatt tttgattggt ttagatcttg ttaaggataa aaaaatactt gaatgcgcat
     3601 ataatgattc agagggtatc actgcacaat tcaatcttaa tattttatca cgaatcaatt
     3661 cagaactcgg tggtaatttc gatctccaga aatttagaca tcatgctgta tataatgaag
     3721 atcaaaacaa aattgaaatg tatttgcgtt caatagacag acagaccata agcatacctc
     3781 aagctgattt aaccttgaat cttgaaaagg atgaactgat acacaccgaa aactcgcaga
     3841 aatttacaat ttcaaatatt aaacagatat cagaaaaagc tggatttgaa attaaagata
     3901 tgtggtatga tgaaaaacag tattttgcta tgatattact ctcaaaaaat gactaaacat
     3961 cctctacaca tctgaatccc gagaataacc atcgttcatc taatctgaaa aaatttctat
     4021 aactactacg tattgacgat gatggtgtac cgaaagatcc tcctctcagc actttttgat
     4081 ttgcaaacca cttgtcatta tactcagaaa aacctgtctt aaatccagga taaccagtga
     4141 attctgagca tgtccactcc caaacatccc caatcatttg atgacaacca taatgacttt
     4201 taccttcagg gtatgcacca atctctgtac aattccacaa gtatgattct aacagatttg
     4261 catgcttgat atctggtgat tcattacccc acggaaagat agacttgctt tggctttttt
     4321 cattccagca tgcagctttt tcccattctg cttctgttgg gagtcttttt ccagcccatt
     4381 tacaatatgc atcagcctcg taaaagctaa cgtggcaaac aggttcgttt ggattgatct
     4441 ttcgtctccc caaaaaatca catgtcatcc attgaccatc aaccttttcc caatacatag
     4501 gggcattcca tccctcattt tctatttttt cccaaccatc tgaaagccaa taatgaaaat
     4561 catcataacc tccatcctct ataaattcta tatactgtct attggttatt ggatagacat
     4621 caattttata gtcattgagg tagaccttgt gttcaggtaa ttctatatca taacaaaatt
     4681 gatcactaga acagcctagt gggtataaac caccatcaat cttgaccgaa ttatattctt
     4741 gttgtaacgt actcggaaac gtattgtgtg ttactggtct atacttatcc gccaagaggt
     4801 gctgtaaatc atatactaaa agttcttgat gttgatattc atgatttatt gccatcaaat
     4861 acagttgttg tgtttttgca tctaatactt cattttgcaa aatgttacta acgttattag
     4921 ttataatatg aaagtattca aaaatctgat caacagtggg ccttgttgcc aatcctctct
     4981 tatctttatc atgtggttca ccaaattgat gataatacga atttagatat tcattaaatt
     5041 cctctgaata gaattcataa ttttgggatg ttttactcat aacaatttca aataaccagc
     5101 taacatgacc taaatgccat ttcggtggac tcataaaaga agcagtctgt acaacaaaat
     5161 catctttttc tagtgtctgt actagattta acgttctaat tctagtttca ttaaactgtt
     5221 ctagtaaatc tgatttttgc cttgaaactg ttgccaaact aagtatgtct gatattggtt
     5281 atattatatt tttatctaga ctttatagca actgcatttt ttcgctttat gattaataca
     5341 cccaataagt ttatgatcct gacaaccaca aataacacac tttttctttt tgtcattcat
     5401 ttttctatgg cttacaatga gacactattg tatagaactc attcatagta ttctctaatt
     5461 cttcagtggt ttctgctttc ataatttttt ctttaaaatc agcaacccat tttgctgtaa
     5521 atgaattaag tgtaatgcta ccgatagttt gcttgttcaa acactcatcc atcttttcaa
     5581 tatattgtga cttatctatc accatcatat ttattgaatc cataaatgga ttcatgctaa
     5641 aaaaatatgc agacgcagaa aaaccaacta ctataattac aattacacca acagtaaatc
     5701 ttacgtttaa tcccatcaga ctatcaccta atatccttta gcaaatagtg catttgtagt
     5761 gcctgaattt ttacaataaa tgcatgtttc tgatttttgt gtaccgtcaa atggaacaac
     5821 acgcaatcct gcaccagtct cctctttgat cttgtcttca catgtttgat taccacacca
     5881 ggaacagtca atcatttttc cattctttaa ttcttctttg aattgttgat attcagaaac
     5941 tctaacaact ctttcatcaa gaattttttt ggcattgaca aacatttgtt gttgtatatt
     6001 ttctaattct aaatttattg tttcggacac tttatccaaa ctgatgtcat cctttgtttg
     6061 gttatatctt ctagccatca ctacttgatt ttttgcaata tcctttggac ctatttcaat
     6121 tctgattggt atacctttca actcccaatc attaaatttg aatcccggtg tgaggttatc
     6181 cctatcatct aattctaccc gtacgttgtt gctagccaag gaatctttga ttttatttgc
     6241 ttctagtatc attgtttctc tatcttcttt cgagtagtaa attggaatta taacaacctg
     6301 aatgggagat acttttggtg gcaagacaag tcctttgctg tcaccatgta ccataatcat
     6361 accaccaatc aatctccatg acactcccca tgatgcttgc catgcatatg tttcaacatt
     6421 gtttacatca aggtatttca catcaaaagg cttcgagaag ttttgtccta agaaatggga
     6481 tgtacccatt tgaagtgctt taccatctgg cattaatgat tcaagcgtag ttgtataaac
     6541 cgcaccaaca aacttatcct tctcactttt ttttcctgtg attacaggaa tagctaactc
     6601 ttcttcgata gtttttttgt atatgtttaa aatttccatt acctcatttt cagcttcatc
     6661 ttgtgtagca tggacagtgt gtccttcttg ccatagaaat tctgatgttc tgagaaatgg
     6721 ttttgtgcca gtaatctctg ctctcaaagc agaattccaa aaattaattt ttaatggtaa
     6781 atctctccaa cttttgatcc attttgaata aagtgaatat gcaagggttt cagatgtagg
     6841 tcttagtgct aatctgtcac ctatttctga atcaccagat tgtgtaaccc aaaaaacttc
     6901 aggattaaaa ccctcaaaat gttttttttc tttaccaagt aatgattcag gtattaaaac
     6961 aggcaaaaat ccatttctat gaccagtttc tttgagtttt ttatctagtg attctttgat
     7021 agattcccaa atagagtaac catcaggcct tagaacaatt aatcctttaa caggagcata
     7081 gtctgcaagc tccgccttga tcaccacttg agtataccac tcactaaaat catcattctt
     7141 ggatgcagtg ataccaactt ctttgttcaa ctatacattt caatcataat ctacataatg
     7201 agtttttctt acccacaatc ttattatcat aaaaaaatct cacataacat ggcaaaaagt
     7261 acaaactggg taaaagtcgt agagaaaatt ctatcgtttg ataaaaaaat gagatttgtc
     7321 gctataattg atctaaaagg aaacatcaca gaaggaatca tgaaagaggg aaaatcttcc
     7381 ttggaatccc aaaggcatca agagcatttt tgtgcacaag ttgcacttag aagaaaaatg
     7441 cgcaaagagt ttgataaaag tttaggcgca gtaaattatg ttcatattga gagaaaaaat
     7501 gtgacacaga ttgtagttta cacacgattc aagaccatat ttgtcaccat tgagccaaat
     7561 cttccaatat ctagaaaatt acaaattatt aaaaagataa aacaactggc agcaaaatta
     7621 taacaataaa attttactat aggttatctc ccttacacag aactttccct gtcactctac
     7681 tttgaaaatt cttacaaata aaacatggga tgaattgaac tgtttccttt aaaactgggc
     7741 actccacata ctctcctttc attcttttta acatctttcc actcactcca ttgatcttca
     7801 attttccttt ttcatccatc attcctgatt cttttaattg tccttttact tctttggaaa
     7861 gcagctgttt tttttcacta gtttttattt caacctcgta tttgaaatca agctcatttg
     7921 acatatcatt gcttttgaga tgctcaattt aatattactg atttgggcta aatctaaata
     7981 gatcggctaa tgctgattaa gcttaatatt gaaaagaatt tggtcgaaat atggaacact
     8041 gtgctagtaa tttttgatgt tgagggagtc ctttatgacg ctgaatattt gccgctttta
     8101 gctgaaaaaa tgaataagga aaaggaagta tgggaaatca ccaaaaaagg tatacaagga
     8161 aagatagatt gggaagaagg gttaagagaa cgtgtagatc ttctcaaggg tctggattat
     8221 aaaacatgta aagacgtggc agattccatg ccaataatga ctggtgctag agaattatgc
     8281 agtgcactaa agaatgcggg ttggaaatta atggcagttt ctggtggttt cacaattata
     8341 actgatagac tgaaaactga attaggtata gatcacatat ttacaaatga gcttatcttc
     8401 aatgatgaga aattagacga tgtagttgta aatgtcaatg cagacaagtc caagtctgct
     8461 atatcaaaaa tcacagaatg gaatgaaaaa aaagagaata tcactgtggt agttgatggt
     8521 gcaaatgatg taaaactgtt tgatatttct gagcttggga ttgctttcag agcccaagat
     8581 ctggtaaaag acttggcaac agtcacactt gatgagaagg atctcactaa gataattcct
     8641 ataataaata atcattataa tctgacatta agcatgtagg ttcagattta agcaatctta
     8701 atctttttta caaatactta ccaagatata tcatgtcaat ccagattatt ccagtcaaaa
     8761 tacaaaaaga tatcgtccct aatgacagtc ttgtagattt ggtcctagaa tcaaccgaaa
     8821 ttcaggatgg cgatatacta gttttttctc aaaaaatagt ttccaagagt gagggtcgta
     8881 ttctaagttt atcatctgta aatccttcac ttcttgccaa cgggatatcc agttcataca
     8941 acaaagatcc aagattgatg gaattgatac tttcagaaag taaaaggata gtaagaatgg
     9001 aaaatggaat aatcatagtt gaaacaaacc atggatttgt ttgtgcgaat gcaggtatag
     9061 atgaaagtaa tgtgcaggat ggctatgcta cgttgctacc tgaagatcct gataggtcat
     9121 ctaacttgtt gaaagaaaga attgaacaaa agactggaaa aaatatcgct gtaataattt
     9181 ctgatacatt tggaagacca tttaggttgg gtcaaaccga tgttgcaatc ggtgttgcag
     9241 gaattgaacc aatcttagat tatagtggta agcctgatac atttgcaaag atcatgcatg
     9301 ttactgccat agcaattgct gacgagatct gttcagcaac ggaacttgtt atgggcaaag
     9361 ttgaaaagtg cccagttgca ttagttcgaa attacaagtt taatttctcc gatgctaaaa
     9421 ttcagaattt attgcggcca aaacatgagg atttgtttag gtaattctaa ataattacca
     9481 cgtcctaaaa tgtatgttga tatggaatca ttaaaaactg aactgcattg ccataacatt
     9541 ttttctaatg gacatgtagg cgttttagaa ccaattcatg actgtagtgt cacaatttca
     9601 cagcaattag aacaagcaca tatggaagga cttgatgtac tttttgttac taatcacaac
     9661 acaatcgatg gttatgaaca gatgcttgaa tataaaaaaa atcattggaa atttgataca
     9721 ttaaacgtat acccagcgga agaggtcaca actgataacg aatcacatgt attggtatat
     9781 gggattgatc acgcaatcaa gcctggactt tcattgcatg aaatattaga tgaggcaaaa
     9841 aaacagaatg ctgttactat tgcacctcat ccattcagtt taatggatgc acttcgtgag
     9901 gattctgttc actgtgatct atttgaggta ttcaacagct caaatgttga tgtatactcg
     9961 aatattaagg caaaaaaatt ttcagaagag aaaaatttga atgtagttgc agggagtgat
    10021 tctcatgtaa aatctacaat tggtcgttgt accaatttaa ttcactctga aaataaatta
    10081 gatgacataa ttcatgcaat gagacataat cagataaaaa tcaacacaac tggatatgta
    10141 caacctaggg aagtattaga gcatattaga tacaagattg aaaattcaaa aaattatatc
    10201 gatatacatg tttcagaatt ttatccacga tcagcatggg cattttcatt attatacaaa
    10261 ttgtacatgc ttagtcctga gggtgtattt tgcaatgcgt tataccgaat atcaataagc
    10321 atgctcaaac ggatttccaa gaaaataaat tttgaaggat acaatccttc tatttttaga
    10381 gaacgtgatc ttggaactat agcaaagatg atcttttgat tatatggtat agattttaat
    10441 tgatgaaagg taaaaaacaa cgtaggtgaa ttgaaatttc agaatcaggg aaaacactag
    10501 acgcaagagg attgttctgt ccatcgccag tgatgcaaac aaatgttgaa ctctcaaaaa
    10561 tgcagattgg tgaaatttta actattcttg ccgatgatcc tgctgctgaa gatgacatag
    10621 caagctgggc tagaagactt gggcatgaaa tagtcaagat ggaaaaaaat ggaaatgaaa
    10681 ttcattttga aattaaaaag gtaaaataaa ctaagacata gtagaactag atacttaaat
    10741 taaataacaa agtgatccta gtataatcaa tgactggtca aaggattgat ggtattgagg
    10801 tgtcaaatgc agttaaagaa attctgtcac gtgcagtcac ccatattaaa tcagaaggct
    10861 tgatgccatg cttggctaca gttctcatag gtgatgatcc tgcatctgct acatatgtaa
    10921 aaaacaagca ca
//
LOCUS       DASCPJ010000131        11482 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002024380, whole genome shotgun sequence.
ACCESSION   DASCPJ010000131 DASCPJ010000000
VERSION     DASCPJ010000131.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002024380"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(163..807)
                     /locus_tag="QGF01_06280"
     CDS             complement(163..807)
                     /locus_tag="QGF01_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HJN20525.1"
                     /translation="MSVPEAVREIITRNRSIYDCMKMDVINYTALAVKIQPGVEKQLG
                     NPVHLNTIVVAIKRYADSFQDEEKVVEEPLLKNARFSLTDKILGMQWTMNDLINEDMG
                     KMFAEAQNAFSNSSFFKLGDSFRILVDDSDDARRIFQTFPKENLYKDGLAKIKIQVPI
                     HNRANVISYVTEILHHNGIELVDALISQDGIVLILNESEAPLAYEKLRSEISSK"
     gene            934..1428
                     /gene="hsp20"
                     /locus_tag="QGF01_06285"
     CDS             934..1428
                     /gene="hsp20"
                     /locus_tag="QGF01_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HJN20526.1"
                     /translation="MFHDDEFNSIFKQMSNSFMNLDDVFEMLKNAGNVTGPIFYGYTM
                     TTGPDGKPVVNEYGNVKPDMLPTEEKREPLIDTLVDEKEKTLKIVAEMPGVEKSDVNV
                     VVGVDRVIHIDAERGEKKYHVKVPINHKVESGSPKATYKNGILELVFKLEDEKSNGTT
                     VNVL"
     gene            1475..1636
                     /locus_tag="QGF01_06290"
     CDS             1475..1636
                     /locus_tag="QGF01_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20527.1"
                     /translation="MTRSYMIIQQLQSSMSLSTQERSRAAKLGHTRRQLAAARRSLAS
                     SKGWLKRKH"
     gene            complement(1710..1976)
                     /locus_tag="QGF01_06295"
     CDS             complement(1710..1976)
                     /locus_tag="QGF01_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20528.1"
                     /translation="MGHKLSELRDLKEMYENRLKSENLEKSLKNNYQTMLDTINEKIE
                     KNQIFRRYFNQRIEKSEICPSCQKEMLSHDKDQALQCMRNFTQN"
     gene            complement(2025..2378)
                     /locus_tag="QGF01_06300"
     CDS             complement(2025..2378)
                     /locus_tag="QGF01_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HJN20529.1"
                     /translation="MPFERRPREMHDAKCGDCGKDCQVPFEPRQDKPVYCDECFQNHK
                     PARSSGGFGGRDRGSRYGGRNDRNSRGSSYERRPREMHKATCADCGNECEIPFKPKDA
                     RPVYCNECFQKHKQN"
     gene            complement(2444..3094)
                     /locus_tag="QGF01_06305"
     CDS             complement(2444..3094)
                     /locus_tag="QGF01_06305"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HJN20530.1"
                     /translation="MKFIDLTLPVSEKIPTFPGSPQPIFIQWENIKDDGYNLELLFLS
                     SHTGTHLDAPYHFIEKGKKIHEISLKRLIINAILVKIRKRGDQPITKTDIQKFEKKHG
                     KIPNGSTVIFWTGWQKMIKNDSYFVSNPGLSVTAAKYLISKKTNLVGIDSPSIDLGKE
                     KRFSVHHLFAKNDVLIVENLANLDKIKSWKFQLAVLPMKLKNATGAPVRAVGIIDD"
     gene            3156..3416
                     /locus_tag="QGF01_06310"
     CDS             3156..3416
                     /locus_tag="QGF01_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HJN20531.1"
                     /translation="MPTAYILINSDLGTDSSIIEYLNKTLSEEKDIQFEVHGVYGVYD
                     IIVKLTSENGETLRGIITNKVRKIDHVQSTLTMMVIDEQDKS"
     gene            3458..4048
                     /locus_tag="QGF01_06315"
     CDS             3458..4048
                     /locus_tag="QGF01_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20532.1"
                     /translation="MSKNPEQVNSKIESILEQNLINIIYYENSFAKAKFLTKTIEKWD
                     TPTFYLDFDLLYSGYVKANMVPTLKNVTLLWPNSENLRDNLESVIEKISMTKSVVVID
                     SLNGFFNTLEDDDAGMLINSFIMMLASSAKNTKSIILVGSLSKLNDENEFVLYSSGRH
                     VIDNKHMKKIQLVESNSLLKINILNFDNSTKSSFSA"
     gene            4157..4456
                     /locus_tag="QGF01_06320"
     CDS             4157..4456
                     /locus_tag="QGF01_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJN20533.1"
                     /translation="MPVAILPDISEQMCIGCALCVEICTTLGPDVLRVKPVEGWKRGK
                     AFVFYPERCISDGACIGVCPTKAIFWMRPMDFTVGQPVPLYKNSVFVKGWTELID"
     gene            4518..5012
                     /locus_tag="QGF01_06325"
     CDS             4518..5012
                     /locus_tag="QGF01_06325"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase 23"
                     /protein_id="HJN20534.1"
                     /translation="MTKIGDAKRKLHGMITGKPDNFSWLIPEKLAGSAIPTSFDEIKW
                     VLDEGVKSIVTIREEPLDDDWIKNVNYLHVMSNDMGVPQFDDLAFAVDFIHRRLDDKE
                     PVMVHCLAGLGRTGTILASYLVKYENMSADEAIKKVREMRPGSIQSYPQEEMIFRFEK
                     SLNR"
     gene            complement(5013..5165)
                     /locus_tag="QGF01_06330"
     CDS             complement(5013..5165)
                     /locus_tag="QGF01_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20535.1"
                     /translation="MAKFDGIRMQDLVEVQDPDKDGGVTLVFKDDKFLHIKIVDGKLV
                     TESVPE"
     gene            complement(5170..5322)
                     /locus_tag="QGF01_06335"
     CDS             complement(5170..5322)
                     /locus_tag="QGF01_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20536.1"
                     /translation="MAKYDGLLGQPLLEVEEPDKEGGLTLIFKDNRFLFVKIEDGKLN
                     TISIPE"
     gene            complement(5346..5879)
                     /locus_tag="QGF01_06340"
     CDS             complement(5346..5879)
                     /locus_tag="QGF01_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20537.1"
                     /translation="MTSTISNLVLVKDKDAYAADENIKINVKFSLTGDLRNQFNEKNW
                     TEAYVKNDNQFKLKYGIKLVTGGIRKHSPGKEIDTYRKASIFWTRNPKLVNPMKEKRI
                     WVQVAKNFEPFIRLTEEEVQQELFDFDEEIMLSASELGKGVHKISADVSASWHSHTWV
                     EGDDEKTHSNEIEININ"
     gene            5927..6193
                     /locus_tag="QGF01_06345"
     CDS             5927..6193
                     /locus_tag="QGF01_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoL/Rpb11 RNA polymerase subunit family
                     protein"
                     /protein_id="HJN20538.1"
                     /translation="MDIEVMESSKNEVNLSIKGGDLGVLYIVQNELLNDPKTDYAGVI
                     TRHPLTNEIWMRVASTNPIKDILKATDSAIAEANKLKKLLTKKG"
     gene            6256..6414
                     /locus_tag="QGF01_06350"
                     /pseudo
     CDS             6256..6414
                     /locus_tag="QGF01_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
     gene            6449..7195
                     /gene="pcn"
                     /locus_tag="QGF01_06355"
     CDS             6449..7195
                     /gene="pcn"
                     /locus_tag="QGF01_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HJN20539.1"
                     /translation="MEFSAKTNTSDEWKAVISSITTLVEEATFEATVEGITFRGMDPS
                     HVALIDISWPNSAFEKYNCDSDIKFGVRVDEFSKVIKRAEKNESIEISISDDNMLQIL
                     IGKNKKWSMRLIEGSASDTPLPKISYDSKIGLSSSTFDKILGDIDVVSDYLFIKTAAN
                     NAEFSGKGDSGEAVINFEKGMEGLEEITVNQECTGTYSLEYLNPIVKAMGNTSDSIIC
                     EFSSGKPLRIEFKVSNIGRIHFYLAPRVES"
     gene            7231..8634
                     /locus_tag="QGF01_06360"
     CDS             7231..8634
                     /locus_tag="QGF01_06360"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HJN20540.1"
                     /translation="MRLVLKFGGTSLSSPNHIRNVAKIVASFSKDNEIVVVCSAVDGT
                     TDDLLTISRLIEQKKKDDVTKVLNNVIKKHKQFANQTVKNSAIKKQLLQKLNTDVSEL
                     KELVRGLTLLKEVSARSLDYLISFGERLSDDLVSFALQDIKKKSTALNGKEVGIVTDS
                     NFGESRPLMDTTRIRISKTLGSLLAKKIIPVVGGFAGADQHGNITTFGRGGSDYTATI
                     IASCINADEIWLMSDVDGLMTADPKLVKNAKLLKEVSYAEAIEMAQFGAKQIHPRTFE
                     PLFHKKIPMRIRSTFDVKNSGTIVTPSPSAATKKTVKCVSAIRNIGLIDLSGGIMFAA
                     PGTAAKIFTTLAEKNVNVMMVSSNPSESSVSLLVKRPDLEKAANALEMNLLGQTVKKV
                     DVTPGVSIIAVIGSGMRGTVGVASKVFSAAQKKNVNVIMIAQGSSELNLAFVVKDSDC
                     KAVVQSLHDEFKLDKIR"
     gene            8676..9461
                     /gene="cobJ"
                     /locus_tag="QGF01_06365"
     CDS             8676..9461
                     /gene="cobJ"
                     /locus_tag="QGF01_06365"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HJN20541.1"
                     /translation="MAGKLYIVGVGPGHHDHMTFRAKQVIEESGTIVGYSTYVNLVQD
                     LIEGKDIHRYDMTQEVERAHQCIKLAKEGKIVSLVSSGDPGIYGMAGLIFEILAESGW
                     NPKTGLDVEIVPGVSALNSCASLIGSPLMTDFAVVSMSDLLVPWEVIVKRVEAAAQGD
                     YVIVIYNPSSKKRIHQLQDTRKLLLKYRSPNTPVAIIKGAYRESESITMTDLEHMEEF
                     ADKLGMISTVIVGNSSTYNFNDLMINPRGYKSKYNIQEPNIQN"
     gene            complement(9458..10504)
                     /locus_tag="QGF01_06370"
     CDS             complement(9458..10504)
                     /locus_tag="QGF01_06370"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HJN20542.1"
                     /translation="MDITEKINLISRPPIEEVVTKEELLELLKTNSNPKHYIGIEISG
                     FLHLGSLISTGFKINDFIQAGVNCTVFLADWHTLLNDKLGGSFENIRKVSKYYEDAFK
                     LVCPGVNIVLGTDLYDSKKEYWSELVQIAKHMSLARTMRTLTIMGRSENDEKIDLAKL
                     LYPAMQAADIHSLDLDIVHAGMDQRKIHMLVKDVFPKMKWKVPVAVHHKLLPGLTKPA
                     EVKPDGEVAKMSKSDPNAGVFIHNSDDEIRTKIKKGFCEEGSIENNPVLEIAKNVIFH
                     EFDTVSIERPEKFGGNVSYDNFASMESDFAQKKLHPSDLKQAVGESLVKIISPIREKL
                     SLSEELSNLIKNSC"
     gene            10576..11175
                     /locus_tag="QGF01_06375"
     CDS             10576..11175
                     /locus_tag="QGF01_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJN20543.1"
                     /translation="MINDVEELIALGAGDKYRLNDTRERLERGKKLYISDLEYLEKLV
                     KTHLGRRLSPEPRKPKRVIRQKPVEGVGSDSCAKCGSKLGSDEKFCTNCGAKKMESKP
                     QQSKPSQPQTKSGKKSRKQELEEYEKKYLAGTDEGFTINEDGEIGEQTSRKTEKPKEQ
                     SIEQPKEEQKKDSAFGMCVKCGSAISAEDLFCTSCGAKR"
     gene            11372..>11482
                     /locus_tag="QGF01_06380"
     CDS             11372..>11482
                     /locus_tag="QGF01_06380"
                     /inference="COORDINATES: protein motif:HMM:NF016310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HJN20544.1"
                     /translation="MELTREEEKSLNGEHGESLQIAYRILSAVGEATDAER"
BASE COUNT         3779 a         2055 c         2090 g         3558 t
ORIGIN      
        1 gcagtcatgt caaccagcgg agttgcattg acaacctttg tttcgctacc ttcttccaga
       61 tgtggaacct tactccacac ttcctgctca aagcgctcaa ctagtgcatt atcaacatct
      121 tcagtcatgt gatcaatgag atttgaggtt taatttagag atttacttgc ttgaaatctc
      181 agatctgagc ttttcatagg ctaaaggagc ctcagattca ttcaaaatca gcacaattcc
      241 gtcctgggaa atcagcgcat ccaccagctc gattccatta tgatgtaaaa tctcagtaac
      301 atagctgatc acattagccc tgttgtgtat aggtacctgg atcttaatct tggctagacc
      361 atccttgtac aaattctcct ttggaaacgt ctgaaaaatt ctcctagcat catctgaatc
      421 atccaccaga attctgaacg aatcacctaa tttgaaaaaa ctagaatttg aaaatgcatt
      481 ttgagcttcg gcgaacattt tgcccatatc ctcatttatc aaatcattca tcgtccactg
      541 catccctaaa attttgtcag ttaatgaaaa tctggcattc ttcagtaatg gctcttcaac
      601 aactttctcc tcatcttgaa acgaatcagc atagcgcttt atcgcaacca caatcgtatt
      661 gaggtgaaca gggtttccca actgtttttc aaccccaggc tgaattttca cggcaagagc
      721 agtatagttg attacatcca tcttcataca gtcatagatt gacctgtttc tggtgattat
      781 ttcacgaaca gcctcaggaa cagacatgcc aatggttctc atggtaggta aatataatgt
      841 catacatatt aatatattgt aatattacct atgactagga aataaactat atataatgta
      901 atacatatga cattaatagg tgaaatatta caaatgttcc atgacgacga attcaatagt
      961 atcttcaagc agatgtctaa ttcattcatg aatttagatg atgtatttga gatgctaaag
     1021 aatgcaggca atgtgacagg cccaattttt tatggttata caatgaccac tggtcctgat
     1081 ggcaagcctg tagtaaatga gtatggaaat gtcaagccag acatgcttcc tacagaagag
     1141 aaaagagaac cactgataga caccctagtt gacgagaaag aaaagacact caaaattgtt
     1201 gcagaaatgc caggtgtcga gaaaagtgat gtcaacgttg ttgtcggagt cgatagagtg
     1261 attcacattg atgcagagcg tggtgagaaa aaataccatg tcaaagtgcc aatcaatcac
     1321 aaggttgaat caggctcccc caaagcgaca tacaaaaatg ggatacttga actggtgttc
     1381 aaactagagg acgaaaaatc aaacggtaca accgtcaacg tactctaatc tctttttttt
     1441 ctattctaaa aataatcaaa agtatcaatg aatcatgaca agatcatata tgattataca
     1501 acaattacag agttcaatga gtctatcaac acaagagcga agtagagcag caaagctagg
     1561 tcatacaaga cgacaacttg cagctgcaag aagaagtctg gcatcttcaa aaggctggct
     1621 aaaacgaaaa cactgatagg aaaaccgagc tcttagatta ttggatcgta gacatgggcg
     1681 aatttggtgt ttttcgtgcc taaaaatcac taattttggg taaaattacg catgcattga
     1741 agagcttgat ctttgtcatg tgataacatc tctttttgac agctggggca aatttctgat
     1801 ttctctattc tttgattaaa gtatcttcga aaaatttgat ttttctcaat tttttcatta
     1861 atcgtatcca gcatagtttg ataattattt ttcaaacttt tttctaaatt ctcagatttt
     1921 agtctatttt catacatctc tttcaaatcc cgtaactcac ttaatttatg acccatgatc
     1981 tatcaccttg tttttctaga ataataaatt gatgatttta cgtattagtt ctgtttatgt
     2041 ttttgaaaac attcgttaca ataaacaggt cttgcatctt ttggtttgaa tggtatctca
     2101 cattcatttc cacaatctgc acaggttgcc ttgtgcattt ctcgtggtct tctttcatag
     2161 cttgaacctc tgctatttct gtcatttctg ccaccatatc tggaacctct atctcggcca
     2221 ccaaatccac cgcttgatct tgcaggttta tgattttgaa aacattcatc acaatagaca
     2281 ggtttgtctt gtcttggttc aaatggaact tgacaatctt ttccacaatc tccacacttt
     2341 gcatcgtgca tttctcgtgg tcttctctca aacggcattt tcttctaata atcgactttt
     2401 ttccccctaa ttatactaag ggatctgact cattctacaa tcatcaatca tctattattc
     2461 caactgccct aaccggagca ccagttgcat tttttaattt cataggtagt acggcaagct
     2521 gaaacttcca agatttaatc ttatccagat tagccaaatt ttccacaata agcacgtcgt
     2581 tctttgcaaa aagatgatga accgaaaatc gtttctcctt tccaagatca atacttggtg
     2641 aatcaatgcc aaccaggttg gtttttttgg aaatgaggta ctttgcggct gtaaccgaaa
     2701 gaccaggatt actcacaaag taagaatcat tttttatcat tttttgccaa ccagtccaaa
     2761 aaatgacagt agacccattt ggaatttttc catgcttctt ttcaaatttt tgtatgtcag
     2821 tttttgttat tggctgatct ccacgctttc ttatttttac caaaattgca tttatgatca
     2881 atcttttgag ggaaatttca tggatctttt ttcccttttc tataaagtgg tatggggcat
     2941 caagatgtgt tcctgtgtgt gaactgagaa acaaaagttc cagattatat ccatcatcct
     3001 tgatattttc ccattgaatg aagattggct gtggagagcc tgggaaggta ggaatttttt
     3061 ccgagacagg caacgtaaga tcgatgaatt tcaatgtcat aattcttgtt tactttttaa
     3121 ttaaaagtta aatattgttt tgtgctgttt tgtgtgtgcc aactgcatat attctcataa
     3181 attctgattt aggaactgac tcatcaatca ttgaatattt gaacaagact ctgtctgagg
     3241 aaaaggacat tcaatttgaa gttcatgggg tttatggtgt gtatgacatt atagtaaaat
     3301 taacttcaga aaatggagaa accttgagag ggataattac caacaaagtc agaaagattg
     3361 accatgttca atcaacattg acaatgatgg taatagatga acaggacaaa tcatagattc
     3421 taaattaatt atatatctgt aaataatcag tgatttgttg agtaaaaatc cggagcaagt
     3481 aaattctaaa attgaaagta tacttgaaca aaacctcata aacataattt attatgaaaa
     3541 ttcttttgca aaagccaaat ttctaacaaa aacaatagaa aaatgggata ctccaacatt
     3601 ttatcttgat tttgatttac tttattccgg ctatgtgaag gcaaacatgg ttcctacact
     3661 caaaaatgtt acattgttat ggcctaacag tgaaaattta cgtgataatc ttgaatctgt
     3721 aatagaaaaa atttccatga caaaatctgt tgtagttatt gattcactga acggattttt
     3781 taacacacta gaagatgatg atgctggcat gttgattaat tcatttatta tgatgttggc
     3841 ctcatccgca aaaaacacta aatctataat tcttgttggg agtctttcaa aactaaatga
     3901 tgaaaatgag tttgtattgt attccagcgg aagacatgta atagacaata aacatatgaa
     3961 aaaaattcaa ttggtagaat ccaacagctt gctaaagatc aatatattga attttgacaa
     4021 ctcaaccaaa agcagtttct cagcctgatt ctatatttct agaatgaacc aaccagcgat
     4081 caaaatttgc taacgccgtt ataatcaacc atttttgagg taattatatt aagctcaatt
     4141 tgtaagaaaa aatacaatgc cagtagcaat actaccagat atcagtgaac aaatgtgtat
     4201 tggatgcgca ctatgtgttg aaatttgcac aactttggga cctgatgtac taagagtaaa
     4261 accagttgaa ggctggaaac gtggtaaggc ttttgtattt tatccagaaa gatgcatctc
     4321 tgatggtgca tgtataggag tttgcccaac aaaggcaatc ttttggatga gaccaatgga
     4381 cttcactgta ggtcagccag ttccactgta caaaaactca gtcttcgtaa aaggttggac
     4441 tgaattaatc gactaaacca accatttcat ttctttttct tatctaattt tatattgtta
     4501 aaacatgtat tttttgtatg acaaaaattg gtgatgccaa acgaaaactt cacggtatga
     4561 ttactggaaa acctgataat ttttcatggt tgattccaga aaaattagct ggtagtgcaa
     4621 ttcctacatc atttgatgaa attaaatggg tacttgatga aggcgtaaaa tcaattgtaa
     4681 caattagaga agagcctctt gatgatgatt ggattaaaaa cgtaaactat ctacatgtaa
     4741 tgtccaatga catgggggtt cctcaatttg atgaccttgc ttttgctgtt gattttattc
     4801 atcgaagact agatgataaa gagcctgtca tggttcattg tcttgctggt ttggggagaa
     4861 ccggaacaat tttagcttct tatcttgtaa aatatgaaaa tatgagcgca gatgaagcta
     4921 taaagaaggt acgtgaaatg agacctggtt caattcaatc atatccacaa gaagagatga
     4981 tttttcggtt tgaaaaatct cttaatcgat aactattccg gaactgattc agtaacaagt
     5041 tttccatcaa ctattttgat atgaagaaac ttgtcatctt tgaagacaag tgttacgcca
     5101 ccatctttgt caggatcttg tacctcaacc aaatcttgca ttcttattcc atcaaacttt
     5161 gccatatgat cactcgggaa ttgaaatggt gtttaactta ccatcttcta tctttacaaa
     5221 caaaaatcta ttatccttaa aaattagagt gagtcctcct tccttatcag gttcttctac
     5281 ctccaaaagg ggttgtccta acaagccatc atactttgcc ataattgttc aaaaatctgg
     5341 tatcattaat taatgttaat ttcaatttcg ttggaatgag ttttctcatc atctccctca
     5401 acccatgtat gtgagtgcca agaagcagaa acatctgcgc tgattttatg aactcctttt
     5461 cccaattcag atgcgctgag catgatctct tcatcaaaat caaacaattc ctgctgaacc
     5521 tcctcttcgg ttaatctgat aaatggctcg aaattttttg ccacctgaac ccaaattctt
     5581 ttctccttca ttgggtttac aagctttgga tttcgtgtcc aaaaaattga tgcctttcga
     5641 taagtgtcta tttcttttcc gggactgtgt tttcttattc cacccgtaac aagtttgatg
     5701 ccatatttca gtttgaattg attatcattc ttaacataag cctcggtcca gtttttctca
     5761 ttaaactgat tacgaagatc tccagtcaat gagaacttta cgtttatctt gatattctca
     5821 tcagccgcat aggcatcttt gtctttcacc aataccaaat tcgaaatcgt tgaagtcatt
     5881 actactgata atgatttata accacaataa gttctttttt gattacatgg atattgaagt
     5941 aatggaatca agcaaaaatg aagtgaatct ttcaataaaa ggtggagatt taggcgtatt
     6001 gtatattgta caaaatgagc tacttaatga tcctaaaaca gattacgccg gagtcataac
     6061 tagacatcca cttacaaatg aaatatggat gagagttgct tccaccaatc caatcaaaga
     6121 catattgaaa gctacggatt ccgcaattgc agaggcaaac aagttaaaaa aactgcttac
     6181 taagaaaggg taatcctgat gggggaaccc gaagaagatc ctgattttat cgtatatgat
     6241 gaagatgaca tgaacgaagc aaatgcacta aaatcaacta taaagattga ctgtgaaaaa
     6301 tgtcatggca aagaaggagt ttggtggagt tttcagactc gtagtgctga tgaacctgaa
     6361 acacggtttt acaaatgtat aaaatgtaat catacatggc gtgattacac ataacgttta
     6421 attttgatat tacaattaat taattaaatt ggaattttca gctaagacaa atacatctga
     6481 tgaatggaag gcggtaattt catcgatcac aactttggtt gaagaagcaa catttgaagc
     6541 aactgtagaa gggatcacgt ttcgaggaat ggatccttct catgtcgcac tcatagacat
     6601 ttcttggccc aactccgctt ttgaaaaata caactgtgat agcgacataa aatttggagt
     6661 tcgtgtagac gaattttcta aggtaatcaa aagagcagaa aaaaacgaaa gtattgagat
     6721 cagtatatcc gatgataata tgttacagat tctgataggg aaaaataaaa aatggagtat
     6781 gagattaatt gaaggctcag cttcagacac acctctccca aaaatttctt atgattctaa
     6841 aatcggtctg tcttccagca cattcgataa aatattgggc gacattgatg tagtctcaga
     6901 ttatctgttt ataaaaacag cagcaaataa cgcagaattt tctggaaagg gagattctgg
     6961 agaggctgtc ataaattttg aaaaaggtat ggagggattg gaggaaatta ccgtaaacca
     7021 agaatgtact ggtacataca gcctggaata tcttaatcca attgtgaagg caatgggtaa
     7081 cacatctgat tccataatat gtgaattttc tagcggaaaa ccacttcgta ttgaatttaa
     7141 ggtatccaat attggcagaa ttcacttcta tctggcacca cgggtagaaa gttaacgaat
     7201 ttattgatta actaaaatag gtatgatact ttgagactag tcttaaaatt cggtggaaca
     7261 tcgctttcat ctccaaatca catacgaaat gttgcaaaaa tagttgcttc attttcaaag
     7321 gataatgaga ttgttgtggt atgctcagca gttgatggta caactgatga tcttcttaca
     7381 atttcaagat tgattgaaca gaaaaagaag gatgatgtta ccaaggtgct gaataatgta
     7441 atcaaaaaac acaaacagtt tgctaatcag acagtaaaaa attctgcaat caaaaaacaa
     7501 ctcctacaaa aactaaacac agatgtttca gaattgaagg agttagttcg tggtcttaca
     7561 ctattgaaag aagtctcagc tcgttcatta gactacttga tttcattcgg tgagagactt
     7621 tccgatgatt tagtgtcttt tgcattacag gacatcaaga aaaaatctac tgcattaaat
     7681 ggtaaagaag taggcatagt taccgattct aattttggag aatccagacc attaatggat
     7741 acaaccagaa ttcgtatttc caagacattg ggttccttac tggcaaaaaa aataatacca
     7801 gttgtgggtg gttttgctgg cgctgatcaa catggtaaca ttacaacatt tggtagaggt
     7861 ggttcagatt atactgcaac cataattgcc tcctgcataa atgcagatga aatttggttg
     7921 atgagtgatg ttgatggctt gatgactgct gatcccaaac ttgttaaaaa tgctaaacta
     7981 ttgaaagaag tttcatatgc agaggcaata gagatggcac aatttggtgc aaaacaaatt
     8041 catccaagaa cgttcgaacc tttattccac aaaaaaatac ctatgaggat tcgaagtacg
     8101 tttgatgtga agaatagtgg aactattgta actccatcac cttccgctgc aactaaaaaa
     8161 actgtcaaat gtgtttctgc aatcagaaat attggtttga ttgatctaag cggtggaata
     8221 atgtttgcag caccaggtac tgcagcaaaa atattcacaa cactagcaga gaaaaatgtt
     8281 aacgtgatga tggtttcttc aaacccatca gagtcaagtg tatcattgct tgtcaaaaga
     8341 ccagatcttg aaaaggctgc aaatgcactt gaaatgaact tgctgggaca aacagtcaaa
     8401 aaagttgatg ttacaccagg cgtttcaatc atagcagtaa ttggctcagg catgagaggt
     8461 acagtaggcg ttgcttccaa ggtattttct gcagctcaaa aaaagaatgt caatgttatc
     8521 atgatagctc aaggatcctc cgaactcaat ctagcatttg ttgttaagga ttctgactgc
     8581 aaggctgtgg ttcagtcact acatgatgaa ttcaagctgg ataagatcag ataacatttg
     8641 aaagagataa attatttaca atcttgagca gaatattggc aggaaaatta tacattgttg
     8701 gtgtaggacc aggtcaccat gatcatatga cttttcgcgc aaagcaagtt attgaagaaa
     8761 gcggcacgat tgttggatat tcaacatatg tgaatttggt tcaagatttg attgaaggaa
     8821 aagacattca cagatatgat atgacacagg aagtggaacg tgcacatcaa tgcataaagt
     8881 tagccaagga aggaaagata gtctcactag tttctagtgg tgatcctgga atttatggaa
     8941 tggcaggatt aatttttgag atactggcgg agtctggctg gaatccaaag acgggactgg
     9001 acgtggaaat cgtaccaggt gtttccgcac taaattcctg tgcatcatta attggttcac
     9061 cactcatgac agattttgca gttgtaagca tgagcgatct tcttgttcca tgggaggtta
     9121 tcgttaaacg ggtagaagct gcggcacaag gtgattatgt tatagtaatt tacaatccgt
     9181 ccagcaaaaa aagaatacac cagttacagg acaccagaaa acttttactg aaatacagat
     9241 caccaaacac tccagttgca atcatcaaag gcgcttatcg agaaagcgaa tcaataacca
     9301 tgacagatct tgaacacatg gaagaatttg cagataagct tggcatgata agtactgtaa
     9361 ttgttggaaa ttcttccaca tacaatttta atgatttaat gatcaacccc agaggctaca
     9421 aatcaaaata taatattcaa gaacctaata ttcagaacta gcaactattt ttaattaaat
     9481 tcgacaactc ttcactcagt gataatttct cccgaattgg tgatattatt tttaccaatg
     9541 attctcccac agcttgcttc aggtcagagg gatgaagttt tttctgtgca aaatcagatt
     9601 ccatgcttgc aaaattatca taactaacat taccgccaaa tttttcaggt ctttcgatag
     9661 atacggtatc aaattcatgg aagattacgt tctttgcgat ttctagtacg ggattgtttt
     9721 caatgcttcc ttcctcacaa aatcccttct tgattttggt tctaatctca tcatctgagt
     9781 tatgaatgaa gactccggcg ttaggatctg atttgctcat ctttgccacc tcaccatcag
     9841 gttttacttc tgcaggtttg gtcagtccag gtagtaactt gtgatgaaca gctactggaa
     9901 ccttccattt catttttgga aatacatcct taaccaacat gtgaattttt ctctggtcca
     9961 ttcctgcatg cacaatgtcc agatcaagcg aatgaatgtc tgctgcctgc attgcaggat
    10021 aaagaagttt tgcaagatca attttttcat cattttctga tctccccatt atggtaagag
    10081 ttctcatagt tcttgccagg gacatgtgtt ttgcaatctg taccagttct gaccaatatt
    10141 cctttttgga atcatataga tcagttccta aaactatatt cactccaggg cagaccagtt
    10201 tgaacgcatc ttcgtaatac tttgaaactt ttctaatatt ttcaaaactt cctcccagct
    10261 tgtcatttag caatgtatgc caatcagcca aaaagacagt acagtttaca ccagcctgaa
    10321 taaaatcatt aatcttaaac ccagtgctaa tcaaacttcc aagatgtaaa aatccagaaa
    10381 tttctattcc gatgtagtgt tttggatttg aatttgtttt taataactct agcaactctt
    10441 ccttagtaac aacttcctca atggggggtc ttgagatcag gtttattttt tcagtaatgt
    10501 ccattttact tgattttttt ttgtaagcct ataaagttta attttcagta aataaaaaga
    10561 aaatccttga gatctttgat aaatgacgta gaggaactca tcgcacttgg agctggtgat
    10621 aaataccgac taaatgacac aagagaaaga ctggagaggg gaaaaaaact ctatatctct
    10681 gatttggaat acttggaaaa attagttaaa acacatctgg ggagaagact tagtcctgaa
    10741 cctagaaaac ccaaacgtgt aatcagacaa aaaccagtcg aaggagtagg atctgattca
    10801 tgtgcaaaat gtggatcaaa acttggttcg gatgaaaaat tctgtactaa ctgtggcgca
    10861 aaaaaaatgg aatcaaaacc tcagcagtcg aaaccaagcc aaccgcaaac taaatctggt
    10921 aagaaatcac gaaaacagga actggaagaa tatgagaaaa aatatcttgc aggtacagat
    10981 gaaggtttta ccataaatga agatggcgaa ataggagaac aaacatccag aaaaacagaa
    11041 aaaccaaaag aacagtctat tgagcaacct aaagaggaac aaaagaaaga ttctgcattt
    11101 ggtatgtgtg taaagtgtgg ctctgcaatt tctgccgaag atttgttttg taccagttgt
    11161 ggagcaaaaa gatagctgcg attgtatcaa attgtatcga aaattttgcc gtaactaacc
    11221 ttattggcat gctagtctaa catatgtttg gtaaaatttg tcattctgta ctaactgtgg
    11281 gcaatcacta aaaccagaag caaagttttg tggcaaatgt ggcgctccac gctcgtaatc
    11341 actattatag agaaatgcct aatcatgatt attggaatta acaagagaag aagagaaatc
    11401 acttaacggg gaacatggtg aatcgctgca aatagcatat agaatattat ctgcagttgg
    11461 cgaagcaaca gatgcagaaa ga
//
LOCUS       DASCPJ010000132         4179 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002072667, whole genome shotgun sequence.
ACCESSION   DASCPJ010000132 DASCPJ010000000
VERSION     DASCPJ010000132.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002072667"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="sucD"
                     /locus_tag="QGF01_06385"
     CDS             <1..857
                     /gene="sucD"
                     /locus_tag="QGF01_06385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HJN20545.1"
                     /translation="QPVIVQGITGKFGSVHTKLMLEYGTNIVAGVTPGKGGQKFEDVP
                     IYETVTEAVSATGAKISIVFVPAKFFLNAAKEAFEAGIKLLVAIPEHVPVRDTMQAIE
                     IAKQNNAIMVGPNTPGIIIPNLIKIGIMPASPFAEGNIAVLSKSGTLLYEISNSLTTS
                     GFGQIFTLGIGGDPINGTRMIDAFDMIKDKSELDGIVIVGEIGGEAEETLAQHIIDIE
                     FKKPIVAYIAGRKAPREKRMGHAGAIIMGNYGSAESKIAMFSKANIPVAKRPTEVPIL
                     LAGKIRKK"
     gene            889..1059
                     /locus_tag="QGF01_06390"
     CDS             889..1059
                     /locus_tag="QGF01_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011277641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HJN20546.1"
                     /translation="MPVTDPEKKQIAQRARLHMKICFNCGARNSLAATRCRKCHNKYL
                     RLKNRALGVKKT"
     gene            complement(1065..1694)
                     /locus_tag="QGF01_06395"
     CDS             complement(1065..1694)
                     /locus_tag="QGF01_06395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HJN20547.1"
                     /translation="MINEKIQNVLLRLEKDIDYENIHRNEVPSEKRLNCISKNIGMFY
                     NVLLKSINAKKILEIGMSVGYSGLWFADVVMSGTQSDGQIITIDREKFKIDKATQNFE
                     EAGVRSLIKIKQGEARKILHEIKEEFGENYFDFIFIDADKESYIEYFDLCLPLVRKGG
                     IIGADNILFPERFNEMMGDYLSHVRNNPNVQSITVPIDNGEEITIKLSE"
     gene            1730..2404
                     /locus_tag="QGF01_06400"
     CDS             1730..2404
                     /locus_tag="QGF01_06400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJN20548.1"
                     /translation="MAEPAKNLVPKFFDETGTTYDGVVSYGTLGKDKYWKKKILEQIS
                     DGSSFLDLACGTGILTREIAAKFPNAKIVGIDITKSYLDVAKQNSNSFDNISFILDDA
                     EEFKLDSKFDCITASYLPKYCDPGILVKNCIMHLNPGGKIIFHDFTYPKNPAVRGLWN
                     FFLTILRLVGYFIPSWKDALIGLPKLIRSTNWLHNYSDVMKKSGLKVSNQYLTYGAAA
                     ILTGTK"
     gene            2433..3506
                     /locus_tag="QGF01_06405"
     CDS             2433..3506
                     /locus_tag="QGF01_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HJN20549.1"
                     /translation="MKALVYENYMENDDFESVLELRDIPEPVPKPNEVIFRVKAAALN
                     YDDIWGMRGKPLAVPLPHISGTDAAGEVIAIGDNIKNIKIGDRIVSHGNMSCRVCAVC
                     TDGREYDCRQRKIWGFETGPLWGGYCEIAHLPEINVVKIPNNVSYEEAAAASMTLLTS
                     WHMLVGRAKIKPGQTVLIMGGSSGVGIFGIQIAKLYGCTVIATASSNKLEKLKELGAD
                     YAVDHRKEDWHKEVRGITKEIAKRDGVSPGVDIIFEHIGGSHFNKELTLLKYGATLVT
                     TGATTGYDVPTDLRHIFFKGINILGSTQGTRAELEEGFHWMGQGKIKAIIDSVFTFEN
                     AVEAHKKMLNGKGLVGKIILKPN"
     gene            complement(3503..>4179)
                     /locus_tag="QGF01_06410"
     CDS             complement(3503..>4179)
                     /locus_tag="QGF01_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20550.1"
                     /translation="DRTSEWFVPKFGSRNFRVTIGLLFLPYTSIVTCFAAWGSLSGSF
                     ETERLVAVCVIYFLALGVSAHLLDAVGGKTKPWGDLPKRKLWTVSMIVLGVAFTIGMY
                     YAFLDSPLLFAIGIAEGFFLFAYNLELFGGRFHNNSSTIISWAILPVFAGSVIQTNSI
                     SLETIILCGISSVITYILITTSRKYKHLRRNNKSQSEIKRCETILKLLTIGVLVGTAI
                     FLVVRF"
BASE COUNT         1354 a          761 c          852 g         1212 t
ORIGIN      
        1 aacaacctgt tatagtacaa ggtataactg gtaagtttgg atctgtacat acaaagttaa
       61 tgttagaata tggtacaaac attgttgccg gtgtaacacc tggaaaaggt ggtcaaaaat
      121 ttgaagatgt tccgatttat gaaacagtga cagaggctgt tagtgcgact ggggcaaaaa
      181 tatctattgt ttttgttcct gcgaaattct ttttgaatgc tgcaaaggaa gcatttgaag
      241 cggggattaa acttcttgtt gctattccag aacatgtgcc agttcgtgat accatgcagg
      301 ctattgaaat cgctaaacag aataatgcta ttatggttgg accaaatact ccaggcataa
      361 ttatcccaaa tctcatcaaa attggaataa tgccagcaag tccatttgca gaaggcaata
      421 ttgcagtttt atcaaaaagt ggtacattac tttatgaaat atctaacagt ctaacaacct
      481 caggctttgg tcaaattttt acacttggaa taggtggaga tcctatcaat ggaacaagaa
      541 tgattgatgc ttttgatatg atcaaggata aatctgagct tgatgggatt gtcatagttg
      601 gtgaaatagg tggtgaggct gaagaaactt tggctcagca tattatagat attgaattca
      661 aaaaaccgat agttgcgtat atagctggta ggaaagcgcc aagagaaaaa cgcatgggtc
      721 atgccggtgc aattattatg ggaaattatg gttcagcgga atccaagata gcaatgtttt
      781 ccaaagccaa tataccagta gcaaaaagac ctacagaagt acctattctt ttagctggaa
      841 agattagaaa aaaatagttt tatacgtagg acaaagtggg aaaacagcat gcctgtaaca
      901 gatcctgaaa aaaaacagat tgcacaaagg gcgcgacttc atatgaaaat ctgttttaat
      961 tgtggggcaa gaaactcatt agctgcaaca agatgtagaa aatgccacaa taagtatctc
     1021 cgtcttaaaa atagagcgtt aggagtaaag aaaacataaa actgttattc tgaaagtttg
     1081 attgtgattt cttcaccgtt atcaattggt acagtaattg actgaacatt tggattattc
     1141 ctaacatgag ataaataatc tcccatcatc tcgttgaatc tttcagggaa caagatatta
     1201 tcagcgccaa ttattccacc ctttcttact aatggcaagc acagatcaaa atattcgata
     1261 taactttcct tatcggcgtc gatgaagata aaatcaaaat aattttcacc gaactcctct
     1321 tttatctcat gcaaaatttt tctagcctca ccctgcttaa ttttgattag agatctcact
     1381 ccagcctcct caaagttctg tgttgcctta tcaatcttaa atttctccct gtcgattgtg
     1441 ataatttggc catctgattg agtacctgac atcactacgt cggcaaacca gagtccagaa
     1501 tagcctacag acatacctat ctcaagaatt tttttagcgt ttatggattt tagtagtaca
     1561 ttataaaaca tcccaatatt ttttgatatg cagttcagtc tcttctcact tggaacttca
     1621 ttcctgtgga tgttctcata atcaatgtcc ttttctaatc ttaataatac attctggatt
     1681 ttttcattta tcattattct ttaaattact tggtgtgtga tatataacat tggcagaacc
     1741 ggcaaaaaat cttgtaccta aattttttga tgagaccggg acaacatatg atggtgttgt
     1801 ctcgtatggt acgttaggca aggacaaata ctggaagaag aagatattgg agcaaatttc
     1861 tgatggaagt tctttcttgg atctggcatg tggtactgga attcttacca gggaaattgc
     1921 ggcaaaattt cccaatgcaa agattgttgg tattgacata accaaaagtt atctggatgt
     1981 agccaagcaa aattcaaatt catttgacaa tatctcgttc atccttgacg acgcagaaga
     2041 attcaaacta gattcaaagt ttgactgcat tactgcgtcc tatttgccaa agtactgtga
     2101 tccgggaata cttgtaaaaa actgtataat gcatctcaat cctggtggca aaatcatctt
     2161 tcatgatttc acatatccaa aaaatccagc tgtcagagga ctgtggaatt tctttcttac
     2221 catcctgcgg ttggtgggat attttattcc aagttggaaa gatgcactaa tcggactgcc
     2281 aaagctgatt cgaagtacca attggcttca caattattct gatgtaatga agaaaagcgg
     2341 attgaaggtg agtaatcaat atcttacata tggtgcagca gcaatcctca ctggcacaaa
     2401 ataagtatga tgtattttta aaaatctcag agatgaaggc attagtttat gaaaattaca
     2461 tggagaatga tgattttgaa tcagttctag agttacgtga cattccggaa cctgtgccaa
     2521 aaccaaacga agtaattttt cgcgtaaagg ctgcggcatt aaactatgat gatatatggg
     2581 gtatgagagg aaaaccactc gctgttccat taccacatat ttctggaact gatgctgctg
     2641 gagaagtaat tgcgattggt gacaacataa aaaatatcaa aattggagac agaatagttt
     2701 cccatggaaa catgtcatgc cgtgtctgtg cagtatgtac tgatggtaga gaatatgatt
     2761 gccgacaacg aaaaatttgg ggatttgaaa ctggaccact gtggggaggc tactgtgaga
     2821 ttgcgcatct tccagaaatt aatgtcgtaa aaattccaaa taatgtaagt tatgaggaag
     2881 ctgcagctgc atcaatgaca cttttaacgt cttggcacat gttagttggg cgagccaaga
     2941 ttaaaccagg tcaaactgtc ttaattatgg gcggtagttc tggtgttgga atttttggaa
     3001 tacaaattgc aaaattatat ggttgtacag ttattgctac cgcaagttct aacaaactgg
     3061 aaaaactcaa agaacttggt gcagattatg ctgtagatca tagaaaggag gactggcata
     3121 aggaggtcag aggaataacc aaagaaattg caaagagaga tggtgtctct ccgggagttg
     3181 atataatctt tgagcatatt ggcggctcgc attttaacaa agaactaact ttactgaaat
     3241 atggtgcaac acttgtaact actggtgcta caactggata tgatgtgcca acagacctgc
     3301 gccacatatt tttcaagggc attaacattt taggctccac acagggaact cgtgccgagt
     3361 tagaggaggg atttcactgg atgggtcagg gaaagataaa ggcaattatc gattccgtct
     3421 ttacatttga gaatgcggtg gaggctcaca aaaagatgct caatggaaaa gggctggtcg
     3481 gaaaaataat tctcaagcca aactagaatc tcacaacaag aaatattgca gtgccaacca
     3541 aaactccaat cgtaagaagt tttagaatgg tttcacatct tttaatctct gattgacttt
     3601 tattgttacg cctcagatgt ttgtactttc ttgatgtcgt tatcaaaatg taagttatga
     3661 ctgaagagat tccgcaaagt atgattgttt ccagcgagat tgagtttgtc tggattaccg
     3721 agccagcaaa aaccggcagt attgcccacg aaattattgt tgacgagttg ttgtggaatc
     3781 ttcccccgaa caattccaaa ttgtatgcaa acaggaagaa tccttccgcg attcctatcg
     3841 caaacaacag aggtgaatcc aagaaggcgt agtacattcc aattgtaaat gcaactccca
     3901 gcacaatcat tgatacagtc caaagtttcc tcttcggcaa atctccccac ggctttgtct
     3961 ttccgccaac cgcgtccagc agatgagctg agacaccaag tgccagaaaa taaatcacac
     4021 aaactgcaac gagtcgctct gtttcaaaag agccagagag cgaaccccat gccgcaaagc
     4081 atgtaacgat gctagtgtat ggcaaaaaca gcaggccaat tgtcactcga aaattcctag
     4141 agccaaattt tggaacaaac cactctgatg tcctatccc
//
LOCUS       DASCPJ010000133        13607 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002077676, whole genome shotgun sequence.
ACCESSION   DASCPJ010000133 DASCPJ010000000
VERSION     DASCPJ010000133.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002077676"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="QGF01_06415"
     CDS             complement(<1..772)
                     /locus_tag="QGF01_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HJN20551.1"
                     /translation="MSENKLGSESSPYLLQHAKNPVHWYAWDDEALARAKKENKPIFL
                     SVGYSSCHWCHVMAHESFEDEETAKIMNENFVNIKVDREERPDLDDVYQKVCQMSTGQ
                     GGWPLSVFLTPNQRPFYVGTYFPAMDSYGRPGFGSLCRQLAQSWKEKPKDVEKAADNF
                     VQNLDKLQPTTTTSSVDKSIFDEAAVNLLQIADPIHGGFGQAPKFPNASNLSFMFRYS
                     KLSGISKFQEFALKTLKKMAKGGIFDQIGGGFHRYSTDT"
     gene            complement(813..1040)
                     /locus_tag="QGF01_06420"
     CDS             complement(813..1040)
                     /locus_tag="QGF01_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20552.1"
                     /translation="MDYRLTAEDKERIKLLDEVTKNKFQNLSLEQLIRLQELVKKKDY
                     SHEKQANKSKKKLLNQINVEIYKRDDSAIWK"
     gene            1119..2156
                     /locus_tag="QGF01_06425"
     CDS             1119..2156
                     /locus_tag="QGF01_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HJN20553.1"
                     /translation="MPKKMRAMVLSKLGPVESNPLKLTEIDRHEIANPKEVLVKIEAC
                     GVCHSQLHSIEGDWEDIGIPPTLPTVPGHEIVGKIVEVGNNVRKFKIGDRVGITPLLS
                     SCLDCQYCNDGKEYLCEKMDVTGESLKGGYTEYITVSEDFATKVPDTMKPEYAAPLFC
                     AGITAYKAVKAAEPKKNNKIAIFGVGGVGHMAIQFAKVEGCEISAISRKNKHLELAKK
                     LGAKNVFVYSSNQEQFLEDVNKKYGLFDAAIVFAPVDQVTDTAIKSIKKGGLVVIATV
                     GKIPNFLAFEEKTVRGTLIGSRKDMEDVIKISDENKLEVITETFPLEQVNQALIKLKN
                     SEMDARAVLIP"
     gene            2182..2331
                     /locus_tag="QGF01_06430"
     CDS             2182..2331
                     /locus_tag="QGF01_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20554.1"
                     /translation="MNCIKCHHVDEAHLSVENDSLLKRGKCMIPTCDCKEYSVAIKKI
                     DEDLI"
     gene            2368..2601
                     /locus_tag="QGF01_06435"
     CDS             2368..2601
                     /locus_tag="QGF01_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20555.1"
                     /translation="MEKHIAHDDIVKKLDIMISRINGLEASSTDEYQRSMSSVLKTLA
                     QGELNMFQELEHMKKALDLLTLELFKIKNKTDA"
     gene            complement(2608..2949)
                     /locus_tag="QGF01_06440"
     CDS             complement(2608..2949)
                     /locus_tag="QGF01_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20556.1"
                     /translation="MAIQILQYEFLGPIRLSEWGPPMDKVVYIIFTKNKEVFNMLYVG
                     ESDKTEELDFFTKNPKFKCWISHAGNEENIYLSIYPMWESSESERLQLAQKIVNKYEP
                     ICNQAVDDSQN"
     gene            3061..3384
                     /locus_tag="QGF01_06445"
     CDS             3061..3384
                     /locus_tag="QGF01_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20557.1"
                     /translation="MKEPHHQRKVGYGMIMVAASLSLIGLLQLTIGGDVLFGDTIQRQ
                     QVAVFEDCKVSDFQEPQCAKWIDELQVQECIETRDVDSSECWKYRTWVIAHAEQELLL
                     SEKEN"
     gene            complement(3396..4091)
                     /locus_tag="QGF01_06450"
     CDS             complement(3396..4091)
                     /locus_tag="QGF01_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20558.1"
                     /translation="MSNWLSSDETDSLFARHSTFVIFCIAASSALILFVALKPIIEDV
                     TSETPMIINIMFVPTFIIGFLYGLKITERVMNPSDARSPIKRGIVKIFLFFFVIGGLF
                     SSVNFALHGGSMVPDGAIFDVGVIDWLIKLVTYNGGATFLIVSSIVLMAAATRKIVGL
                     GGGYLGKIISFVGTFVFFSMVSVSLTQNDPTHSQVFLYTFFYAGIISGALYEMNKLTT
                     KQNMWEDYSNGYL"
     gene            complement(4174..5370)
                     /gene="truD"
                     /locus_tag="QGF01_06455"
     CDS             complement(4174..5370)
                     /gene="truD"
                     /locus_tag="QGF01_06455"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HJN20559.1"
                     /translation="MIPKIDSEIGITVYSTKFSGSGGTIKNQNDDFIVSEIITEKAHA
                     MINEDSGYAVYKLKKNGIDTTHALNEIFKKYGVRLKALGLKDASAITEQFVFTTNKTR
                     RDFEVQEPKYSLKKIGFSSKPLSKKNMIGNHFQIKINDPTTTLCDFNEYDKILNFYGY
                     QRFGSQRPVTHLIGKSLIQKDFGMAVELLLSFTSQYDTSKNTEIREVMKDKSRFSYAM
                     SIIPNGMDLEKIVLKQMIEHNDPVLAIRALPLSIRRFFVQSYQSYIFNKTLSASFENN
                     EELFSPQENDVCYDKNGNLGKFENDPYQRLSIPFVGYSYYKKTRFHHYIKKILEDEQI
                     TEKDFFVKEMQEISNDGGFRNSSIKCDNYKLEDNIVSFSLSRGSFATILLREIIKPDK
                     PLLSGF"
     gene            complement(5367..7022)
                     /locus_tag="QGF01_06460"
     CDS             complement(5367..7022)
                     /locus_tag="QGF01_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20560.1"
                     /translation="MKTRTKGIIFFAFVGGIMLASGVTIAIPSSFFENSDTALDSLDD
                     IDLDSLDYVGDQRLQFCGVNDKAKSNSYVKEYKIPTPCTQPLAITTMPDGTVWFAQTN
                     TGMIAKFDPPSETFTQYKNPDWDRLQTVWIEEALLQNVLPIKLRSMMWGMDYSPDGSI
                     WYTDGDNGAIWKFLLSDESYHVLSFPREDDSEQVFPQKLAVDGSRIIVNDLFGSKLSF
                     FDFVQVGQAIRTFAIPSPIAGSLTSDFTIDSKDNVWYTTWIPNETGILVKFDYPKYEK
                     QQAISSTITDGLLLQEFIEFYQFPSGMDTPNGIAVGPNEKIWIADTSGNFFFSFDPEI
                     EKFTKYVTSIPHEDSYGNLKLPTYSSNPYWIEQLDEKLVMNEHNANRIAVFDPIKETM
                     VEYTVPSRNTSWADCEGIEYCGVAQVFGFTVDDKKIWFTEWVENNIGVVDTSIPLPFD
                     INLNRENIILERGETTEITLEFSSTDTTPYGHLVNISTTSLFTDLAINPESTEFYSND
                     SGSESIIIQITASDSALPDTHKVLLGAYNDEIAISKFITVTIE"
     gene            complement(7077..7430)
                     /gene="pth2"
                     /locus_tag="QGF01_06465"
     CDS             complement(7077..7430)
                     /gene="pth2"
                     /locus_tag="QGF01_06465"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HJN20561.1"
                     /translation="MMTIKQVIVVRNDLDMGKGKIAAQVGHACVLGAEHVRKSNPEWF
                     SKWWSGQEKVVLKVSSLKELEQIKQDAIELGLPWSEVTDAGHTQISPGTTTCISLGPA
                     PEEKIDKITGNLKLL"
     gene            7509..8651
                     /locus_tag="QGF01_06470"
     CDS             7509..8651
                     /locus_tag="QGF01_06470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJN20562.1"
                     /translation="MDKYDIVIAGGSISGLLTAREVAKKGYSVLVLEEGFEIGTPDHC
                     GGLVSKTALNDLGINTSEKIYDCAINNASIFSPSGKTLEINSKNQQIISVDRRQLDKQ
                     AAYQAQKYGAKIIVNSSYKEKTKEGVSTSIGNIDCKILVDCRGVGALVNNNRNGILLS
                     AQYEVYADWIVNGHVEVYFDQIKYPGFFGWIIPSGNGIGKVGVAGKDINTSKTIEEFL
                     KGKGKYSTIRKIFSPIWINGPIKNFVSKDVVTVGDAAGQSKPTTAGGIYSCGMGGIMA
                     GKAIAKYLETNDDFQLMQYQESWYSKFGKEFEKQQLARKVLQKIDNKTVDKIFDMITP
                     EILSEISSKDDFDFHTASIIKLLGVRKSLAAAQNLMGSEIKRLLVG"
     gene            8705..8887
                     /locus_tag="QGF01_06475"
     CDS             8705..8887
                     /locus_tag="QGF01_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019374.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HJN20563.1"
                     /translation="MSPSLVLPICSCCHRNIMPNDKCVKFNCPNCEKGDLIWRCESCR
                     VAVRNYKCSVCNFEGP"
     gene            8890..9168
                     /locus_tag="QGF01_06480"
     CDS             8890..9168
                     /locus_tag="QGF01_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HJN20564.1"
                     /translation="MAQLLLITKILPTGIDVDLDGLIETIRTNLKDGIKLRNFQKEPL
                     AFGLNYLKSEFILDDKEGQMDSLENTLHSVDGVSEFEVLNMSRMSVDV"
     gene            9177..11348
                     /locus_tag="QGF01_06485"
     CDS             9177..11348
                     /locus_tag="QGF01_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HJN20565.1"
                     /translation="MPVKEEPLQMRVGESKQRDVGKKRARIGPDAMDYMHVAPGDIIE
                     ILGKKTTSVIVWPADEDEKNPDIISIDGQTRKNVDVSINDVVTVGKVSTKTAKLVTLM
                     PVNDVVTVDKEFTDFVKNRLKGLPLTNGDEISVMILGNSIEFKISKSSPKGVVKIDRS
                     SNLKILSEAASDKKTRITYEEVGGLGDELKAMREIVELPLRHPELFSRLGVEPHSGVL
                     LYGPPGCGKTLIAKVLANESDANMYLINGPEIMNKYYGETEARLREIFKEAKDNSPSI
                     IFIDEIDAIAPKREEAYGDVEKRVVAQLLALMDGLNERGNVVVLGATNRPDSVDPALR
                     RPGRFDREVEVAVPNTDGRLEILHIHTRGMPLAEDIHLKDFANELHGYTGADIKSLCR
                     ESALKAIRRYLPEIDLENERIPSKMLESMEIKLRDFYDAMFEVVPTAMREFYVERAKI
                     WWKDIGGLDYAKETLEDNMIASINEPDKFTKMGVKPPKGVLLYGPPGCGKTLLGRALS
                     AECGSNMILVRGPEILSKWVGESEKAIREIFRKAKASAPCIIIFDELDSLAKFKAMDG
                     NAQGETVLSQMLTEMEDSGTSRIAVIGITNRPDLIDNSMLRTGRLDITLYIQPPDEKG
                     RLEIIKILTEKMPLNNDVNLKEIAVATQNYTGADLAALCREAAVHAMQNNSKKINSND
                     FALGLKKIKPSITQEINQWYNTLKNEVSNIIPKSTDKTFYG"
     gene            11354..11650
                     /locus_tag="QGF01_06490"
     CDS             11354..11650
                     /locus_tag="QGF01_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20566.1"
                     /translation="MNYKDIIFGKVKEDNSLTDKTLIKSLKKEGHEFTEDMFNKTLLD
                     LEILGLIHVSWIAKDTRKIEIASQYEENDEVELQNKEALKNDYEASFPGTENQT"
     gene            complement(11651..12523)
                     /gene="cyoE"
                     /locus_tag="QGF01_06495"
     CDS             complement(11651..12523)
                     /gene="cyoE"
                     /locus_tag="QGF01_06495"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HJN20567.1"
                     /translation="MVLKEIFEVSKPRIIILLVITAVTSMYAGSKFVGPDLNYIDLMH
                     IIIAGSLASAGSSALNHFYDKDIDPKMKRTSERPIPSGRIGANSVLAYGLGVSILAVV
                     YAYLLINPLSTFFIALGIFFYVIIYTAWLKRKNWSNIIIGGFAGSAASMAGWSAATGS
                     IDPLGFLIGFLVFMWTPSHFWCLAMKIRDDYAAANVPMLPVIIGMEKTSKYILLSTLV
                     LLPYSFMLYAFGMGLVYMAFAVVSGGLMLGYHYKLTKVPTSDFAWKAYKVTAPYLTII
                     FIGIALDAAFHFRF"
     gene            12617..>13607
                     /locus_tag="QGF01_06500"
     CDS             12617..>13607
                     /locus_tag="QGF01_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HJN20568.1"
                     /translation="MSNWELVMPGGGLTAIGMAGLVTSYSGIAHTFIDGMHALTGLLF
                     FIGLIFLSAGILDGGVSTSNRTKATVLVTISIVLTFGAAAFIGSTSTTLPTLTGILIL
                     IAVPSILIAYIAMKMPQYVRPVGIIVCFAVSLGIVCFFVFGIFGPTEYLIVDVVEDVT
                     EEISESIEPSNVFAISILAGSAEQGNPDYKPDNAMIEHGYVIEWTNDDDVMHTVTSSL
                     DFGETFDSGIIDAGNTFELDTTNLELGQYEYMCIVHPWMIATFVLEEPRGPMKEIVSM
                     PMGAANNVEGQVFYDPENITVPKTTIVEWTNDDDVMHTVTSSLDFGETFDSGII"
BASE COUNT         4644 a         2161 c         2472 g         4330 t
ORIGIN      
        1 ttgtgtcagt agaataacga tgaaaaccgc caccaatttg atcaaagatt ccacctttag
       61 ccattttttt taaagttttc aatgcaaatt cttggaactt ggatattcca gaaagttttg
      121 agtatctaaa catgaatgat aaattagatg cgtttggaaa ttttggcgct tgcccaaaac
      181 caccatgtat aggatcagca atttgtaaaa gattaaccgc tgcttcatca aatattgatt
      241 tatcaactga agaagttgtg gtggtaggtt gaagtttatc taaattctgt acaaaattat
      301 cggctgcctt ttccacatct tttggttttt ctttccatga ctgtgccaat tgtctgcaaa
      361 gacttccaaa tccaggtctt ccatacgaat ccatcgcggg aaaatatgtt ccaacataaa
      421 atggtctttg atttggggta agaaacacac tcaatggcca accaccctgc ccagtagaca
      481 tttggcaaac cttctgataa acatcatcca aatcagggcg ttcttctctg tctactttga
      541 tattaacaaa attctcattc atgatttttg cagtttcttc atcctcaaat gattcgtgtg
      601 ccataacatg acaccaatga catgaactat accctacact tagaaagatt ggtttgtttt
      661 ctttttttgc tctagcaagt gcttcatcat cccacgcata ccaatgtact ggatttttag
      721 catgttgtaa cagatatgga ctggattcac tacctaattt gttttcactc ataaaatatt
      781 gaatccaaat aaactatttg tatgtaatct aattatttcc agatagcact atcatcacgt
      841 ttgtaaattt ctacattaat ttgattaagc agtttcttct ttgatttatt tgcctgtttt
      901 tcatgactat aatccttctt tttcactaat tcctgtaatc gtattaattg ctcaaggcta
      961 agattttgaa atttattctt tgtaacctca tcaagaagtt ttatcctctc tttatcttca
     1021 gcagtaagtc gataatccat taataaaaaa tagttttaaa ctgattttaa ttcttattgt
     1081 ttacaaaaat ttaagaacat atatcaaaat taatgattat gcctaagaaa atgcgtgcca
     1141 tggttctttc aaaacttggt cctgtagaat caaatccatt aaagcttact gaaatcgata
     1201 gacatgaaat tgcaaatccc aaagaggttt tggtaaaaat tgaagcatgt ggtgtatgtc
     1261 attcacagct acattccata gaaggtgatt gggaagatat aggtatacca ccaacacttc
     1321 caacagttcc tgggcatgaa attgtgggaa agattgtaga ggttggaaac aatgttagaa
     1381 aattcaagat aggtgataga gtaggaataa ctccattgct tagttcttgt ttagactgtc
     1441 agtattgtaa tgatggtaaa gagtatcttt gtgaaaagat ggatgtgact ggtgaatcac
     1501 tcaagggtgg ttatacagaa tacataactg tctcagaaga ttttgcaacg aaagtacctg
     1561 ataccatgaa acctgaatat gcggcacctc ttttctgcgc tggaataact gcttacaagg
     1621 cggtaaaggc tgctgaaccc aaaaaaaata acaaaattgc aatttttggt gtagggggtg
     1681 tgggtcatat ggctatacag tttgccaaag ttgagggttg tgaaataagt gcgatttcaa
     1741 gaaagaacaa gcatttagag ttagcaaaaa aattaggagc aaaaaatgtt tttgtctatt
     1801 catctaatca agaacaattt ctggaagatg ttaacaaaaa atatggtttg tttgatgctg
     1861 cgattgtttt tgcaccagtt gatcaagtga cagatactgc aattaaatca ataaagaagg
     1921 gaggtttagt agttattgcc acagttggta aaatcccaaa ttttttggcg tttgaagaaa
     1981 aaactgtcag aggtactttg ataggatcaa gaaaagatat ggaagatgta attaaaattt
     2041 cagatgaaaa taaattagaa gtgataacag aaacttttcc tctagaacaa gtgaatcaag
     2101 ctttgattaa actcaaaaat tcagaaatgg atgcacgggc ggttcttatt ccttaaaata
     2161 tgtgtgtcag gtttaaaatt tgtgaactgt ataaaatgtc atcatgtgga tgaggcacat
     2221 ctatcagtag aaaatgattc actcttaaaa cgtggaaaat gtatgatacc aacatgtgat
     2281 tgtaaagagt attctgtagc aataaaaaaa attgatgaag acctaatcta aaaaatagtt
     2341 catatatgaa gtgatagtca atacttcatg gagaaacata tcgcacatga tgatattgta
     2401 aaaaaattag atattatgat atcaagaatt aatggtcttg aagcttcatc taccgatgaa
     2461 taccaaagat caatgtctag tgttcttaag acactggcac aaggtgagct taatatgttt
     2521 caagagttgg aacatatgaa aaaggcactt gatctactca cattagaatt attcaaaata
     2581 aaaaacaaaa ctgatgcatg aatctgctca gttttggcta tcgtcaacgg cttgattaca
     2641 aattggctcg tatttattga caattttttg agctaattgc agtcgttcag attcactaga
     2701 ttcccacatc gggtaaattg aaaggtaaat gttttcctca ttacccgcat gtgatatcca
     2761 acatttgaat ttaggatttt ttgtaaaaaa atccaattct tccgttttat cagattcacc
     2821 aacatatagc atgttaaaaa cttccttgtt ttttgtaaat atgatataaa caactttgtc
     2881 cattggagga ccccactcgg ataatcttat tgggccaaga aactcatatt gaaggatctg
     2941 tatagccata acaattgata aagattctag tttttttctt tttcgaatga tcctataact
     3001 acactagttc caaggatctg ctgaaggatt aaatggtact agtttcgctt caaatataat
     3061 atgaaagaac ctcatcatca aagaaaagtt ggatatggta tgattatggt tgcagcttca
     3121 ctatctctta taggattatt acagttgacc ataggtggag atgttctttt tggtgataca
     3181 atacaaagac agcaagtagc agtttttgaa gattgtaaag ttagtgattt ccaagaaccg
     3241 caatgtgcta aatggataga tgagttacag gttcaagaat gtatagaaac cagagatgtt
     3301 gatagttctg aatgttggaa gtatagaaca tgggtaattg ctcatgcgga acaagaactg
     3361 ctattgagtg aaaaggaaaa ttaagaatga ccgatttata gatagccgtt tgaataatct
     3421 tcccacatgt tttgtttggt tgttaattta ttcatctcat ataatgcgcc agatataata
     3481 cctgcataga aaaatgtata caaaaatact tgtgagtgag ttggatcatt ctgcgtaagg
     3541 ctaactgata ccattgaaaa gaaaacaaat gttccaacaa atgaaatgat cttaccaaga
     3601 taacctcctc ctaaacctac aatttttcga gtggctgcag ccattaaaac tatgctagat
     3661 acaatgagaa aagtagctcc accattgtat gtaactaatt ttattaacca atcaatcaca
     3721 ccgacatcaa aaatagcacc atctggtacc atactgcctc catgtaaagc aaaatttacg
     3781 gagctgaata atcctccaat aacaaaaaag aacagaaaaa tctttacaat tccacgtttg
     3841 attggagatc ttgcatctga tggattcatt acacgttcag taatcttcag gccgtaaaga
     3901 aatcctataa taaatgtcgg cacaaacatg atattgatta tcataggtgt ttcagaagta
     3961 acatcttcaa ttattggttt cagtgcaaca aaaagaatta gagctgatga tgctgctata
     4021 cagaaaatta caaaggttga atggcgggca aatagagaat cagtttcatc tgatgatagc
     4081 caattagaca tgccaaatac taaaaaaatc tggattaaag ataaagtaaa aaaatcatta
     4141 agtcattact taattctgtt tacctatagc agcctagaac ccagaaagca ggggtttatc
     4201 aggtttaatt atttctcgaa gaagaatagt ggcaaaagaa cctcttgaaa gtgaaaatga
     4261 cacaatatta tcttccaatt tataattatc acattttata gatgaatttc taaatccacc
     4321 atcattactt atctcctgca tttctttaac aaaaaaatcc ttctctgtta tttgttcatc
     4381 ctctaaaatt ttttttatgt aatgatggaa acgagttttt ttgtaatatg aatatccaac
     4441 aaagggaata gataatcttt ggtaaggatc attttcaaat tttcctaaat taccattttt
     4501 atcataacat acatcattct cttgtggaga gaataattct tcattatttt caaaagatgc
     4561 actcaatgtc ttattaaaaa tatatgattg atatgattgt acaaaaaatc gccttattga
     4621 taatggtaaa gcacgtatgg ctagaactgg atcattatgt tctatcattt gttttaacac
     4681 aattttttcc aaatccattc catttggtat aatactcata gcataagaaa acctagattt
     4741 atctttcatt acttctcgaa tttcagtatt ttttgacgta tcatattgag aagtaaatga
     4801 cagtaaaagc tcaacagcca ttccaaaatc tttctgaatt aatgattttc caattaaatg
     4861 ggttactggt ctttgggaac caaatctttg gtagccataa aaatttaaaa ttttatcata
     4921 ttcgttaaaa tcacataatg tcgtagttgg atcattaatt ttgatttgaa aatgattgcc
     4981 gatcatattt ttttttgata atggtttaga tgaaaatcca atttttttta aactatattt
     5041 tggttcttga acttcgaaat cacgcctagt tttatttgtc gtaaaaacaa actgctcagt
     5101 tatggctgat gcatccttca aacctaatgc cttaagccta acaccatatt ttttaaaaat
     5161 ttcatttagt gcatgtgttg tatcaatacc attttttttt agcttgtaaa ccgcatagcc
     5221 agaatcttca ttaatcattg catgtgcttt ttctgtgata atctctgata caataaaatc
     5281 atcattttga tttttgattg ttccaccaga accagaaaat tttgttgagt atactgtgat
     5341 tcctatttca gaatcaattt ttggaatcac tctattgtca cagtgataaa tttggagatt
     5401 gctatctcat cgttatatgc gccaagaaga actttatgtg tatctggtaa cgcgctatca
     5461 cttgcagtaa tttgtattat gatactttct gaacctgaat cgttagaata aaattcagtt
     5521 gattcaggat taattgccaa atcggtaaat aatgaagttg tagatatatt taccaaatgt
     5581 ccatatggcg tagtatctgt tgaactaaat tcaagtgtaa tttcagtagt ttcacctctc
     5641 tctaaaataa tattttctcg attaagatta atgtcaaatg gaagtggtat cgatgtatct
     5701 actacaccta tgttgttttc tacccattca gtaaaccata ttttcttatc atcaactgta
     5761 aagccaaata cctgggcaac accacaatac tcaattcctt cacagtcagc ccaactagtg
     5821 tttcttgaag gtacagtata ttctaccatt gtttccttga taggatcaaa tactgctatt
     5881 cggtttgcat tgtgttcgtt cataactaat ttttcatcta attgttcaat ccaatatgga
     5941 tttgatgaat atgttggaag ttttaaatta ccatacgaat cttcgtgtgg aattgatgta
     6001 acatattttg tgaatttttc tatctcagga tcaaaactga agaaaaagtt tcccgacgtg
     6061 tcagctatcc aaattttttc atttggacca actgcaattc catttggtgt atccattcca
     6121 gatggaaact ggtaaaattc aatgaactct tgtaaaagaa gtccatctgt gattgtggag
     6181 gatattgctt gttgtttttc atatttagga taatcaaact ttacaagtat tccagtctca
     6241 tttggaatcc aagttgtata ccatacatta tctttagaat caattgtgaa atcactcgtt
     6301 aaagatccag cgattgggga gggaatagca aaagttcgta tcgcttgtcc tacttgaaca
     6361 aagtcaaaaa aagaaagctt acttccaaac aagtcattaa caattatcct tgaaccatca
     6421 actgctaatt tttgtggaaa gacttgctct gaatcatcct cacgtggaaa tgaaagtacg
     6481 tgatatgatt catcagatag taaaaatttc catattgcac cattgtcacc atcagtatac
     6541 cagattgaac catccggaga ataatccatt ccccacatca ttgaacgcag ttttattggc
     6601 agtacattct gtagaagtgc ctcctcaatc catactgttt gtaatctatc ccagtccggg
     6661 tttttgtatt gagtgaatgt ttctgatggt ggatcaaatt ttgcaatcat tccagtgttt
     6721 gtttgtgcaa accaaactgt tccatctggc atagttgtta ttgcaagagg ttgagtacat
     6781 ggtgtaggta ttttgtattc ttttacatat gaattagatt ttgctttatc atttacgccg
     6841 caaaattgta atcgctgatc accaacatag tctaaggaat caaggtcaat atcgtccaaa
     6901 gaatccaatg cagtatcgct gttttcaaaa aatgaagaag gtatagctat tgtaactcca
     6961 gaagctagca taattcctcc aacaaatgca aaaaagataa ttccctttgt ccgtgtcttc
     7021 ataatgaaaa aactacaata ctacattata ttttattgca ataaatatga aaatgattac
     7081 agtaatttca aatttccagt gatcttatct attttttctt ctggtgcagg accaagtgaa
     7141 atgcaggttg tggttcctgg agagatctgt gtatgtccag catcagtaac ttcagaccat
     7201 ggcaaaccaa gttcaatggc atcctgcttg atttgttcaa gttctttcaa gcttgaaact
     7261 ttgagtacaa ctttttcttg tccactccac catttagaaa accactcagg attggatttt
     7321 cttacatgct cagcacctag cacacatgca tggcctactt gagcagcaat ttttccctta
     7381 cccatatcaa ggtcatttct aactacaatc acctgtttta ttgtcataat gcttacaaga
     7441 attatgtatt atgaaaaact ttgcaatttg gtcgaaacat taattgaata atacagattg
     7501 gagtgtttgt ggataaatat gatatagtta tcgcaggtgg aagtatttct ggtctattaa
     7561 ctgctagaga agtagcaaaa aaaggatatt ctgttttagt tttagaagaa ggatttgaga
     7621 ttggtactcc tgatcattgt ggtggattag taagtaagac tgcattaaat gacttgggga
     7681 tcaatacatc ggaaaagatc tatgactgtg caatcaataa tgcatctatt ttttcaccta
     7741 gtggtaaaac gcttgaaatt aactcaaaaa atcagcaaat catttctgtt gatagaaggc
     7801 aattagataa gcaggcagca tatcaagccc aaaaatatgg tgcaaaaata attgtgaatt
     7861 caagctataa agaaaagacg aaagaaggtg tcagcacgtc aattggtaac atcgattgta
     7921 aaattctagt tgattgtaga ggagttggag cgttagtaaa taataacaga aatggtatat
     7981 tattatctgc acaatatgaa gtgtatgcag attggatagt aaatggtcac gttgaagtgt
     8041 attttgatca aattaaatat cctggttttt ttggatggat aataccatcg ggaaatggta
     8101 taggcaaagt tggtgtagcc ggtaaggaca ttaacacttc taaaactatc gaagaatttc
     8161 taaaaggtaa aggaaaatat tcaactataa gaaaaatatt ttccccaatc tggataaatg
     8221 ggccaatcaa aaattttgtt tccaaagatg ttgtgacggt tggtgatgct gccggacaat
     8281 caaaaccaac tactgcaggt gggatttatt catgtggtat gggtggaatt atggctggta
     8341 aagcaattgc aaaatattta gaaacaaatg atgattttca acttatgcaa tatcaagaat
     8401 cttggtatag taaatttgga aaggagtttg aaaaacagca acttgccaga aaggtactac
     8461 aaaaaattga caataaaaca gttgataaaa tatttgatat gattacacca gagatattat
     8521 ccgagatctc tagcaaggat gattttgatt ttcatacagc atctataata aaattacttg
     8581 gtgtacgtaa atctctagca gctgcacaaa accttatggg atctgagatt aaacgtctac
     8641 tagttggtta accaattaga atgtaataag gtataaatgc agtacatcat cagcaagttt
     8701 tagaatgtca ccaagtctag tattaccaat ttgtagttgc tgtcatagaa atatcatgcc
     8761 taatgataaa tgcgtcaaat tcaattgtcc taactgtgaa aaaggagatc tgatttggag
     8821 gtgtgaaagt tgtagagtgg cagttagaaa ctataaatgc tcagtttgta atttcgaagg
     8881 tccttaaaaa tggcacaatt acttttgatt acaaaaatac ttcctaccgg aatagacgtt
     8941 gatttagatg gattgataga aactatcaga actaatctga aagatggtat aaaattgagg
     9001 aattttcaaa aagaaccact tgcatttggt ctgaattatc taaaatcaga atttattttg
     9061 gatgataagg agggacagat ggattcgtta gaaaatactc tgcactctgt tgatggtgta
     9121 agtgagtttg aagttttgaa tatgagtaga atgtctgttg atgtgtaggt aatatcatgc
     9181 cagtaaaaga agaaccatta caaatgcgag ttggtgaatc taagcaacga gatgtcggta
     9241 aaaaaagagc cagaattggt cctgatgcca tggattacat gcatgttgca cctggtgata
     9301 taattgagat actaggtaaa aaaacaacta gtgtaattgt ttggcctgca gatgaagatg
     9361 aaaaaaatcc agatataatt agtatagatg gtcaaactag aaagaatgta gatgtgtcta
     9421 tcaatgatgt agtaacagta ggaaaggtta gtacaaaaac tgctaaacta gtaacattga
     9481 tgccagttaa tgatgttgta actgttgata aagaatttac tgattttgtt aaaaatagac
     9541 taaaaggatt gcctttgaca aatggtgatg aaatttcagt tatgatcttg ggtaactcta
     9601 tagaatttaa gattagtaaa tcatctccta aaggtgttgt taaaattgac cgttcttcca
     9661 atctaaaaat actttcagaa gctgctagcg ataaaaaaac tcgtataaca tacgaagagg
     9721 tgggtggact tggtgatgaa ctaaaagcga tgagagagat tgttgaattg ccacttagac
     9781 accctgagct ttttagccga cttggtgtag aaccacatag tggtgtatta ctttatggtc
     9841 caccaggttg tggaaaaaca ctcatagcta aagttcttgc taatgaatca gatgctaaca
     9901 tgtatctgat taatggacct gaaattatga ataaatatta tggtgagact gaagctagac
     9961 tgagagaaat tttcaaggag gcaaaggata attctcctag tatcatcttt attgatgaga
    10021 ttgatgcaat tgcaccaaaa agagaggaag cgtatggaga tgttgaaaaa cgtgtagttg
    10081 cacaattatt agcacttatg gatggtttga atgaaagagg taatgttgta gtacttggtg
    10141 caactaatcg tcctgatagt gtagaccctg cattacgaag gcctggaaga tttgatagag
    10201 aggttgaagt tgcagttcct aatactgatg gaagattaga aattctgcat atacatactc
    10261 gtggaatgcc tcttgcggaa gatattcatt tgaaagattt tgcgaatgaa ttgcatgggt
    10321 atactggtgc tgatatcaaa tcgctatgta gagaaagtgc attaaaggct atacgtcgct
    10381 atctacctga aattgatctt gaaaatgaac gtataccatc taaaatgctt gaatcaatgg
    10441 agataaaact acgtgatttc tacgatgcta tgtttgaagt tgtgccaaca gcaatgcgag
    10501 agttttatgt tgaacgggca aaaatttggt ggaaggatat tggtggattg gattatgcca
    10561 aagaaacact tgaagataat atgattgcgt ctattaacga accagataaa tttactaaaa
    10621 tgggtgtcaa gccaccgaag ggtgtattac tttatggtcc accgggttgt ggaaaaacac
    10681 tccttggtag agcattatct gctgaatgcg gaagtaatat gattctagtt cgtggtccag
    10741 aaatattatc aaagtgggtt ggtgaatcag aaaaagcaat tcgagagatt ttccgaaaag
    10801 caaaagcatc tgcaccatgt ataattattt ttgatgaatt agattctctt gcgaaattta
    10861 aagcaatgga tggtaatgca caaggtgaaa ccgtcttgag tcaaatgtta accgaaatgg
    10921 aagatagtgg aacgtcacga attgcagtta taggtattac aaatagacct gatctcattg
    10981 ataattctat gcttcgtacc ggtagattag acatcacgtt gtatattcag ccaccagatg
    11041 aaaagggtcg actggagata atcaaaatat taacagaaaa aatgccacta aataatgatg
    11101 ttaatttaaa agaaattgct gtagccacgc aaaattatac tggtgcagat ttggctgctc
    11161 tttgtagaga agcagcagtt catgccatgc agaataattc taaaaaaatt aatagtaatg
    11221 actttgcctt aggattaaag aaaataaaac catctattac ccaagaaatt aatcaatggt
    11281 ataacacatt gaaaaatgaa gtatcaaata tcatcccaaa atctacagat aaaacatttt
    11341 atggataaaa aatatgaact ataaagacat aatatttggt aaggttaaag aagacaattc
    11401 tcttactgat aaaaccctca taaaaagtct aaaaaaagaa ggacatgaat ttacagaaga
    11461 tatgtttaac aaaactttat tggatttaga aatattagga ctcatccatg taagttggat
    11521 tgcaaaagat actcgtaaaa tagaaattgc atctcagtat gaagagaatg atgaagttga
    11581 attacaaaat aaagaagcac taaaaaatga ttatgaagca agtttccctg gaactgaaaa
    11641 tcaaacctaa ttaaaatcta aagtgaaatg cagcatctaa tgcaatacct atgaatatta
    11701 tcgtaaggta tggagcagtt accttgtatg ccttccatgc aaaatcagat gttggaactt
    11761 ttgtaagctt gtaatgatat cccaacatca aaccaccaga tactaccgca aatgccatgt
    11821 aaaccagtcc cattccaaat gcatagagca tgaaagaata aggaaggagt actagcgtgg
    11881 atagcaaaat atactttgat gtcttttcca taccaattat tacaggcaac attggaacat
    11941 ttgccgctgc ataatcatca cgaattttca ttgcaagaca ccagaaatga gatggagtcc
    12001 acatgaacac caaaaatcca atcaaaaatc ccaatggatc aattgatccc gtggctgcag
    12061 accagccagc catagatgca gcactacctg caaaaccacc aattattata tttgaccaat
    12121 ttttgcgttt aagccatgcg gtatagatga tcacgtagaa aaatattcca agtgcgatga
    12181 aaaatgttga tagaggatta attaacagat aagcataaac aactgccaaa atacttacac
    12241 ccaatccata tgccaaaact gaatttgccc caattcgtcc tgaaggaatt ggtctttcac
    12301 ttgtccgctt cattttcgga tcaatatctt tatcgtagaa gtgatttagc gcacttgaac
    12361 cagccgaagc aagtgagcca gctatgataa tatgcatcaa atcaatataa ttaagatcag
    12421 gacctacaaa cttacttcca gcatacatcg aggtaacagc tgttatgacc aataaaataa
    12481 ttatccgagg tttggaaacc tcaaatatct ctttcagtac caattatctt tccagaatat
    12541 aacaaaattt aatttattcg atagtagaat ttattggcaa aacgattaat tatctaacat
    12601 atttcaacaa gcttgaatga gtaattggga attagtcatg ccaggaggcg ggcttacagc
    12661 gatcggtatg gctggtcttg tgacttccta ttctggtatt gcacacacat tcatagatgg
    12721 aatgcacgcg cttactggtc ttttattctt tattggattg atattcctga gtgctggtat
    12781 acttgatggt ggtgtatcaa cttcaaacag aacaaaagct acagttcttg ttacgatatc
    12841 aatagtttta acatttggtg ctgcagcttt cataggtagt acaagtacta cattaccaac
    12901 tcttactgga attcttatcc taattgctgt tcctagtatt cttatcgcat atatcgcaat
    12961 gaaaatgcct caatacgtaa gaccggttgg aattatagtt tgttttgctg ttagtttggg
    13021 aattgtatgc tttttcgttt tcggtatttt tggaccaact gaatatttaa tcgtcgatgt
    13081 tgttgaagat gttaccgagg aaatttctga aagtatagaa ccatctaatg tatttgctat
    13141 ctcaatacta gctggttcag cggaacaagg aaatcctgat tataaacccg acaatgctat
    13201 gattgaacac ggttatgtta tagaatggac taatgatgat gatgtaatgc acactgtgac
    13261 tagctcactt gactttggtg aaacatttga ttctggaata attgacgctg gaaatacatt
    13321 cgaacttgat acaacaaatc ttgaactagg acagtatgaa tacatgtgta ttgtacatcc
    13381 ttggatgata gcaacatttg tactcgaaga accaagggga ccaatgaaag agattgtaag
    13441 tatgcctatg ggtgcggcaa ataatgtaga gggtcaagtg ttctatgacc cagagaatat
    13501 tactgttcca aaaacaacaa ttgttgaatg gactaatgat gatgatgtaa tgcacacagt
    13561 aactagctcg cttgactttg gtgaaacatt tgattctgga ataattg
//
LOCUS       DASCPJ010000134         1558 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002088815, whole genome shotgun sequence.
ACCESSION   DASCPJ010000134 DASCPJ010000000
VERSION     DASCPJ010000134.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002088815"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="QGF01_06505"
     CDS             complement(<1..280)
                     /locus_tag="QGF01_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012686.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20569.1"
                     /translation="MESLKISILDLLIIVSYFIGVVYLGIWISKKKIKGGDDYFLAGR
                     QMTWPYVGASLFSTNISSQQFVGQAGLAFVGGIAVGTFQMIGALSFGLL"
     gene            complement(314..>1558)
                     /locus_tag="QGF01_06510"
     CDS             complement(314..>1558)
                     /locus_tag="QGF01_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HJN20570.1"
                     /translation="GVRFTDWYSNSPVCSPSRASLLTGRYPGNAGVRAILDGHRTATG
                     LPTEVPTIASALKESGYYTAMSGKWHLGLAEGSRPHDHGFTDWFGFMAGCIDYYSHIY
                     YWEMNKDGKSQYHDLWENGKGIRRNGEYFTELITEYAIKYIRRSLELDKPFLLYIPYN
                     APHYPMHAPEKYLERFPNLKWDRQIMAAMLSAMDDSVGKIIDELKKHDLYDNTITFFQ
                     SDNGPSRESRNWLDGNLDPYYGGSTGKLKGHKYSLFEGGIRSPAIINWPKNIPSGQVI
                     NEPGVAMDIFPTFLTAAGVDLNQYELDGKNILPMVIEGEKSSHDGLFWEMNEQTAVRK
                     DKWKLVLNGHLVEDVPSEDNVHLSDLENDMAESINLKEKYPQITKELKKIAQDWRNGI
                     ENRWENEWLPKANGTTGYVKKK"
BASE COUNT          461 a          334 c          230 g          533 t
ORIGIN      
        1 ccaataaacc aaaactaagc gctcctatca tttgaaaggt tccaactgca atacccccta
       61 cgaaggctaa ccctgcttgt cctacaaatt gctgactaga tatattcgtt gagaataaag
      121 aggctcccac gtagggccag gtcatttgcc gaccggccag gaaataatca tctccccctt
      181 tgatcttttt tttggaaatc cagattccca gatatacaac acctatgaaa taggagacga
      241 tgattagtaa atccaggata cttattttta atgattccat tattgatcct gttttttaat
      301 agaaaataaa aaattatttt ttctttacat atcctgtagt gccattagct tttggtaacc
      361 actcgttctc ccagcgattt tctatcccat ttcgccaatc ttgtgctatt tttttcaact
      421 cttttgtgat ctgaggatat ttttctttta aatttattga ttctgccata tcattttcta
      481 aatcagacag atgtacatta tcctctgatg gaacatcctc cactaaatgc ccatttaaaa
      541 ctaatttcca tttatctttt ctcaccgctg tttgttcatt catttcccaa aataggccat
      601 cgtgagatga cttttcccct tcaatcacca ttggtaaaat attttttcca tctaattcat
      661 attgattcaa atcaacacct gcagccgtta aaaaagttgg gaaaatatcc atagcaactc
      721 caggttcatt tattacttgc cctgatggaa tattttttgg ccagtttatg attgctggtg
      781 accttatacc tccttcaaat aaactgtact tatgtccttt taattttcca gttgaaccac
      841 cataataagg atctaggttt ccatctaacc aatttcgact ctctcttgaa ggtccattat
      901 cactttgaaa aaatgtgatt gtgttatcat ataggtcatg tttttttaat tcatcgataa
      961 ttttacctac actatcatcc attgccgata acatggcagc cataatttgt ctatcccatt
     1021 ttaaatttgg aaatctttca agatattttt ctggcgcgtg cattggataa tgcggggcat
     1081 tatatgggat atacaaaagg aatggtttat ccaattctaa agaacggcgt atatatttta
     1141 tagcatattc agttattaat tctgtaaagt actcaccatt acgtcgaatt cctttaccat
     1201 tttcccataa gtcatggtac tgtgacttac catctttatt catttcccaa tagtaaatat
     1261 gagaataata atcaatacat cctgccataa aaccaaacca atcggtgaat ccatgatcgt
     1321 gtggtcgaga accttctgca agtcctaaat gccatttacc agacatggca gtgtaatatc
     1381 ccgattcttt taaagcactt gcaattgtag gaacttctgt aggtaaccct gttgcagtac
     1441 gatgaccgtc taaaatcgca cgcactccag cattaccagg atacctgcca gttaataatg
     1501 atgctcttga aggagaacat acaggtgaat tagaatacca atcagtgaat ctaactcc
//
LOCUS       DASCPJ010000135         1807 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002124283, whole genome shotgun sequence.
ACCESSION   DASCPJ010000135 DASCPJ010000000
VERSION     DASCPJ010000135.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002124283"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            750..1524
                     /locus_tag="QGF01_06515"
                     /pseudo
     CDS             750..1524
                     /locus_tag="QGF01_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1588..>1807
                     /locus_tag="QGF01_06520"
     CDS             1588..>1807
                     /locus_tag="QGF01_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20571.1"
                     /translation="MSFVDDNIPTIYVGYDPREDLAYKVLKHSALKHASSPLNVYPIK
                     QHILRRIGLYRRAWKLGSSALPKPNNEDD"
BASE COUNT          664 a          293 c          259 g          591 t
ORIGIN      
        1 ctccatccag tcatgagaaa tgttaaggaa gaaacaaagt tatattattg tgttacaaaa
       61 taaaatataa ttgcaataac aatgacagca ccaacaatcc aataccattt tttcatatta
      121 ttacctccca gatttaaaac aaataactct aaaataaaaa aaataattga tccaaatcaa
      181 attattcatt tattaacttt ttattgattt aaatcattta acactcaatg ataaattgct
      241 agtatagttt aacaaaggag gttttgagta tgagtccacc aaaatcggat agggtggtgt
      301 ccacaaccaa gcacagttct ttaaacaatt aagcagacct ttttaaaagg tttccaatta
      361 ggcaaataag ccgaaatata accccatgaa catattgttg catggggttt tctattcatt
      421 cactatttat ttttagtcaa tttatttaac aataaaatta cttttttaaa aacgtgttaa
      481 aatgctttat taatttatca gtggtcttaa actcattttt ttcaatgtat gccagtaatg
      541 aattggggtt aaattgatca aatacataga tattaaataa ttcaatcgct ttggtcataa
      601 gtcctctatt ccacatttct tctcctagcc aataaccaat cacctaattt aaattatttg
      661 atctaaatca attttttttt tattcatctt caattgatct aaatcaataa gaaatatgtt
      721 ctttaaatta gtttatttga attatagtaa tgaaaaattt gaaagaaaca actcatgaaa
      781 acattcaaat ttatatcgga tatgattcta acacagctgt tgcttaccat gtattgtcgc
      841 atagtattca tgcaagagca actcaccctg taagcattac tcctgttaaa ttatcagaga
      901 taaaaaaaat ttataatcat ccatgtgaaa tatttcagtc aacggagtat tcattttcca
      961 gattccttgt tccataccta aacaactatc ggggatgggc tctttacatg gattgcgata
     1021 tgcttgttct aaaagatatc tctaaattat ggtctttgcg tgattctcga tatgcagtga
     1081 tgtgtacaaa acatgatcac attccacggg aaaaaaagaa agctttgggg gtgccgcaaa
     1141 aaatttacga taaaaaaaaa ttggtctagt cttatgttaa tgaattgtac gaagtgtact
     1201 gtacttaccc caaactacgt aaataatacc aaaggtctgg cccttcatca gtttgattgg
     1261 ttaaaatctg ataaacaaat aggaagtatc cctttgaaat ggaatcactt ggtggatttt
     1321 caggaagtac aaccaccaaa taaaatttct aatcttcatt ttacctcagg tggtccgtat
     1381 tttaatgact acactaattg tggttatgca acggtgtgga tcaatgaact gcagaacatg
     1441 attaattcca aacaaactca aaacacaaaa catgttcaaa ccaagaagca atccttttta
     1501 aaattattca agaaacataa ttgaaatgaa caaaatattt aatgataaaa taattttcca
     1561 aacaacatct aaaagaaaga atgtaatatg agttttgtgg atgataatat tcccactatc
     1621 tatgttggtt atgatccacg tgaagatttg gcatataagg tattgaaaca ttcagcacta
     1681 aagcatgcat cttctccatt gaatgtatat cccatcaagc agcatatatt aagacgcata
     1741 ggattgtata gacgggcatg gaagttgggt agctcagcac tacctaagcc aaataatgaa
     1801 gatgata
//
LOCUS       DASCPJ010000136        11383 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002127906, whole genome shotgun sequence.
ACCESSION   DASCPJ010000136 DASCPJ010000000
VERSION     DASCPJ010000136.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002127906"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="QGF01_06525"
     CDS             <1..714
                     /locus_tag="QGF01_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20572.1"
                     /translation="NTKLSDRQYPFFSNFKNKIKIYPISSPSELEIILYANSNNGEEI
                     HSQKIGSLVSPSNEFIDADINAIFENTEINISTISSFGVRAQVINDSEMPTRIGHQLI
                     YGAGGLDSSINVVLGNPNIFNRPGKKSMKWGQIIVGGNFDSFFGFTADSKENKEITKH
                     EANITFYDKNGKLEEKKVDVLNGTSVKFEASKEFKEFTDYDLPQYIWCSAESENYGLN
                     FFACTYNTQTKHCSGDHGF"
     gene            complement(738..1070)
                     /locus_tag="QGF01_06530"
     CDS             complement(738..1070)
                     /locus_tag="QGF01_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20573.1"
                     /translation="MDPDEHRIRQEDCNEDTIGIGILTLTNKRAAFDKKQSRVMDFSA
                     TMGDTVLDVPLENITKVWKEGLLMKKVCFTAKMEDGEKTWKFGVFSNGSWLKTFKKTL
                     ENLESKNN"
     gene            1109..1729
                     /locus_tag="QGF01_06535"
     CDS             1109..1729
                     /locus_tag="QGF01_06535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HJN20574.1"
                     /translation="MKLIRKGAEGDLFFTIWNGQESVLKSRKKKDYRDSSLDNRLRKQ
                     RTVRESEIMHEVKKFGIHVPLIYFVDIKNCNIIMQKIDGVLVHDLSNSKLVNACKKIG
                     KLVGIMHKNGIMHGDLTTSNFIQSKDQIFIIDFGLASRTFKSDDHAVDLRLFKEILNS
                     AHADVFEKAWSNFLSGYRLSVGNERFSQITNKVMVIESRGRYARVV"
     gene            1713..2270
                     /gene="rdgB"
                     /locus_tag="QGF01_06540"
     CDS             1713..2270
                     /gene="rdgB"
                     /locus_tag="QGF01_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HJN20575.1"
                     /translation="MPESYDILFASSNEHKCEEAKEILVEFGIELEFFKTDLVEIQDD
                     SLSKIALQKALNAYEKCKKPVIIEDDGLFIDSLSGFPGPYSSYIFNTIGNNGILKLIG
                     DNRDAQFVAIIAFCDSSNEPVLFESSVAGKISKNIQDGGWGYDPIFIPEKQNKTYAEL
                     YDKNKLSHRYEALKKFANWFNSKQE"
     gene            complement(2251..3270)
                     /gene="twy1"
                     /locus_tag="QGF01_06545"
     CDS             complement(2251..3270)
                     /gene="twy1"
                     /locus_tag="QGF01_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HJN20576.1"
                     /translation="MSCSGEVVEKEPELIQIRSSIVKQLKKAKYGIADHSTVGLCHWT
                     KKSFRNEGTCYKHKFYGISTHSCMEFSPAGMYCENRCIYCWRPMEFYDSMKMERHNVA
                     EPEEIMTKLMAERRKLIMGHYGDPNQDKKKLDESLLPNHYAISLSGEPTMYPKLPELI
                     KYLKSLEATKSIFLVTNGQEPDMIQKLQDENALPTQLYLSTNAADYQSFLKINRPRYD
                     DSWERWNRTLKMLSTLDTRTVLRITLIRGYNDGEEMIPVFASLLQQSNAHFIEIKSYM
                     HIGRSINRLEYSDMLEMDEVRHFANKLSQENGAFTVMDESEISRIVILQNKKRFVDRF
                     IPAYC"
     gene            3355..4407
                     /locus_tag="QGF01_06550"
     CDS             3355..4407
                     /locus_tag="QGF01_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HJN20577.1"
                     /translation="MLKTVSVAGIEEQGIPFKKDSITLEDAVAEYVVENGTLTWIECV
                     VDDIVNETPGILEKLQIDMDPSVLLSGYITAYEDIGDTLGIMLPFIITGDSRTQTTPI
                     LIFMKKDLIVTIHDDYGGKIAKLYNYSNSLMRKLPKESEQWADRQTILLFRLMDEVSE
                     TNFSILRTILEQAEQLEIDISGARQMDRNISDELSNMKRSLLTFLSAVWATHDTVRNV
                     KYGDPDMLTDDDDILEKFEVILGRLDRQIQMAENVMEIISTGVTVIQTETSNQLTKLI
                     VWLTVAATAVLVPNTIATVLGIPDLHISLAWVIPILIISTVISVIVTYRWTKQWRVNP
                     FRSGKFHTFRKKFSRK"
     gene            4614..4685
                     /locus_tag="QGF01_06555"
     tRNA            4614..4685
                     /locus_tag="QGF01_06555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4647..4649,aa:His,seq:gtg)
     gene            complement(4694..5041)
                     /locus_tag="QGF01_06560"
     CDS             complement(4694..5041)
                     /locus_tag="QGF01_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20578.1"
                     /translation="MEERLNINVSATNYDESSKEITTILTMLEEMVHGENEFRMTDSE
                     FAFGWHFYVVSVSLNLIQKLSDQMGDDFYKLKGKGLEKKFLTWLTQKTKQKNLHVKFA
                     IKEEMESSKFGIF"
     gene            complement(5078..5836)
                     /locus_tag="QGF01_06565"
     CDS             complement(5078..5836)
                     /locus_tag="QGF01_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJN20579.1"
                     /translation="MKIEEYIKSLPDNIISGDDVQLPEHSFRNIFEFLELDENDIFYH
                     LGCGDGKGIFIARNEFHVKKAVGIDNDTKKIQQANDYGKKIKLDRWEFFCEDILTAKM
                     DDATVILFWFTDNDLIENMMKKFKDLPDGCRIITLWGPLPQCLPSKVEFPYIVNQVPF
                     KTANLKEQLLEIFDAKCIDFVNAWEYAERYTKAISLSDIENNRFLTILQSLVIWINAK
                     NLGIACGNEIPIPIKNYMGILKTFFNIEVEHLLK"
     gene            complement(5862..6131)
                     /locus_tag="QGF01_06570"
     CDS             complement(5862..6131)
                     /locus_tag="QGF01_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HJN20580.1"
                     /translation="MVSKEQNQELMNNTISTLNQIATNPSTPRNIRKNIANMVIDLES
                     KEYSVSVRAANTISLLDDITQDPNLPSYIRTSLWQAVSTLENIRE"
     gene            complement(6167..6475)
                     /locus_tag="QGF01_06575"
     CDS             complement(6167..6475)
                     /locus_tag="QGF01_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HJN20581.1"
                     /translation="MEKIFVCKTTQISVGQMKKISVNGKDIVVANIGGDYFAINDTCT
                     HAGASLSEGKIEDSTIICDWHGAQFDCKNGKLIKFPAKINDLEVYNVIVEEDNILIET
                     "
     gene            complement(6509..7372)
                     /locus_tag="QGF01_06580"
     CDS             complement(6509..7372)
                     /locus_tag="QGF01_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HJN20582.1"
                     /translation="MKIARLLKDGKETYGIIKNDKVATRQIITYETGVPLPLSIRDFL
                     FDGWYNEIENKISDSSFQDNLKNFQIVEPLPNPSKIICLTFNYPNHAKENNVMSPKDP
                     VIFFKPRTSLCGTGSNIICPSFVKQLDYEIELAVIIGKKIKNVDENKAKDCIFGYMVF
                     NDVSARDIQMQDKQFTRGKSFDTFAPCGPYITTASEIPNPQDLRLVTRVNDQIRQDSS
                     TKNMFISISSIISKLSKVMTLEKGDIITTGTPEGVALNNPDIPFLKDGDKIEMEIEKL
                     GKITNTVKFVD"
     gene            complement(7379..9091)
                     /gene="gltX"
                     /locus_tag="QGF01_06585"
     CDS             complement(7379..9091)
                     /gene="gltX"
                     /locus_tag="QGF01_06585"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HJN20583.1"
                     /translation="MDNDLKKEIRKIALQNAVEHDGGTKDKIVLSKVLGSFPELKNKV
                     KDVMPEIASIVSEVNSIPIEQQKIEIENNFPEILKVKEKVKEDRIGLPPLEDAEQGNV
                     VTRFPPEPNGYPHIGHAKAAIINDEYSKMYGGKNVLRFDDTNPENERLEYWAAIKVGL
                     DWLGIKYDKIKNTSDDIELFYDKCKDLLNQNLAYVCTCKRDEISKNRKEMISCKCSES
                     DPKQNEDGWDKMFHKYKPGEAIVRFRGNMESKNTVMRDPILFRIIDARHPLLEKKYRV
                     WPSYDFAVAIEDSLDDVTHAFRSKEYELRNELYYEILDALNMRKPKMGEFSRLEFKGM
                     PVSKRILKPLIDDGKVSWYDDPRLPTLEALKRKGITPEAIRKFTLSLGFTKSNTLAPF
                     DSLEAFNRKIVDGNSIRLFMVKSPKILTVSNLPHSVVKLPNHPSNDMGVREVTVEGDI
                     LLSSEDVKDLKINDQLRLMGLGNVKITSINSKITAEFTNDNHDVSFRKIQWICQKNAH
                     KLKILIPHQLFIDDKFNEGSLEEVDVYTEPHYLELKDGAEIQFIRFGYCRKDSMNQAI
                     FTHK"
     gene            complement(9093..10076)
                     /locus_tag="QGF01_06590"
     CDS             complement(9093..10076)
                     /locus_tag="QGF01_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HJN20584.1"
                     /translation="MKVSNSIKKNAKFVNKFLRKELNGDPKQLYDAASHLIIHGGKRL
                     RPYLVLKSCQMLGGKQSDAISAASAVEMIHNFTLVHDDIMDNDEMRHGVTTTHKKFDI
                     PLAILAGDVLYSKAYNTISSKSKLSSNYTTQLVSKLSKACIEICEGQVYDIKFAENKK
                     IPTEKEYIKMIEKKTAVLFEVSCAMGAICAKRKQKDVKNLSAFGRNLGIGFQITDDLI
                     GIIGDSKITKKPVGNDIREGKKSLPIILAIKKARGQNRTKIMRVFGNSGASKQQINLA
                     VNVIRSLGVEKEVRDIALKYAQRAEKSISSYTGSAKNEMAALLSFVTKRSL"
     gene            complement(10073..10711)
                     /gene="idi"
                     /locus_tag="QGF01_06595"
     CDS             complement(10073..10711)
                     /gene="idi"
                     /locus_tag="QGF01_06595"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HJN20585.1"
                     /translation="MEEYVILVDENDNPIGKEEKVKCHLPNGKLHRAFTALIFNNEGK
                     LLLTKRSEGKMLWPNDWDGTVASHPRESETYASSAERRMPEEIGISCKMNYVNKFEYH
                     VPYKDVGSENEICGTLIGTIDSFDESSMIKDEISEIKWINPDELKNELERNRDVYCPW
                     MVIALYFLANSDPNTLEKFNSLITKWASDDLKSVYENAIKHYIPDNNWRLVE"
     gene            complement(10716..>11383)
                     /locus_tag="QGF01_06600"
     CDS             complement(10716..>11383)
                     /locus_tag="QGF01_06600"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HJN20586.1"
                     /translation="IDNILKQIKKINEPKILVHGGGSYGHYWSVKYGMHTKPAKYDLK
                     GVSVVKNSMIELNKIILDSAVKNRLNPYCLPPTDFMNGSKPIRSKVEKIGEIAKSGLT
                     PVTYGDALWYGQKKSYILSGDVIMTTIAKVLKPRLSVFVLNVDGVYSNIKSKKLIYDF
                     RKEKPEISKNKMDVTGGMERKITEATKMSKSGLKVFFVNGNKPQRIASAVSGKKFEGT
                     LFR"
BASE COUNT         3523 a         1977 c         1735 g         4148 t
ORIGIN      
        1 aatactaaac tatcagatag gcaataccca tttttttcaa atttcaaaaa taaaattaaa
       61 atttatccta tttcatctcc ttcagaactt gaaataattc tatatgctaa ttcaaacaat
      121 ggagaagaaa ttcattctca aaaaattggt agtctagtta gtccaagtaa tgaatttatt
      181 gatgcagata ttaatgcaat ttttgaaaac acggaaataa atatttcaac aatctcatct
      241 tttggtgttc gggcacaagt aattaatgat agtgaaatgc ctacccgtat tggccatcaa
      301 ttaatttatg gtgcaggtgg tttggattca tctattaatg ttgtattagg aaacccaaat
      361 attttcaacc gtccagggaa aaaaagtatg aagtggggac aaattatagt aggaggtaac
      421 tttgattcct tctttggatt cactgctgat tcaaaagaaa ataaagaaat tacaaaacat
      481 gaagcaaata taacatttta tgataaaaat ggaaaattgg aggaaaagaa agttgatgta
      541 ttaaacggaa catctgtaaa atttgaggct tcaaaagaat ttaaagaatt tacagattat
      601 gatctgcctc aatatatttg gtgtagtgcg gaaagcgaaa actatggatt gaactttttt
      661 gcatgcacct ataataccca aactaaacat tgttctggag atcacggctt ctaatatcta
      721 acacttaatt ttttctttta gttattttta gattctaaat tttctaatgt ttttttaaac
      781 gtctttaacc aacttccgtt actaaacaca ccaaacttcc aagtcttttc accatcttcc
      841 atttttgcgg tgaaacaaac ttttttcatt aataatcctt ctttccaaac ttttgttata
      901 ttttctaatg gtacatccaa tacagtatct cccatagttg ctgaaaaatc cataacccta
      961 ctttgctttt tatcaaaggc agcacgtttg tttgtaagag ttaaaattcc aatacctatt
     1021 gtatcttcat tacagtcttc ttgcctaatt ctgtgttcat ctggatccat gatagatttt
     1081 aatctgttaa ccatagaata taatattaat gaaattaatt aggaaagggg ctgagggaga
     1141 cctttttttt actatatgga atggtcaaga atctgtttta aaatcgagaa agaagaagga
     1201 ttatagagat tcatctcttg ataatcgtct tcgaaaacaa agaaccgtta gagaatcgga
     1261 aatcatgcat gaagtaaaaa aatttggaat tcatgtacca ttaatctatt tcgttgatat
     1321 caaaaattgt aatatcataa tgcaaaaaat tgatggtgtt ttggtacatg atttatctaa
     1381 ttccaagttg gtaaatgcat gtaaaaaaat tggtaaattg gttggtatta tgcataaaaa
     1441 tggaataatg catggtgatc taactacttc caattttata caatcaaagg atcaaatctt
     1501 cataatagat tttggtcttg caagtagaac tttcaaatca gatgatcatg cagttgattt
     1561 aagattattc aaagaaattc ttaacagtgc acatgcagat gttttcgaaa aggcatggtc
     1621 taatttcttg tctggttaca ggttatcagt aggaaatgag cgttttagcc aaattacaaa
     1681 taaagttatg gtaattgaaa gtcgtgggag atatgccaga gtcgtatgat attctttttg
     1741 cttcatcaaa tgagcacaag tgtgaagagg caaaagaaat tctagtagaa tttggaatag
     1801 aattggaatt tttcaaaaca gacttggtag aaattcaaga tgattcgtta tcaaaaatag
     1861 cgctgcagaa ggcattgaat gcatatgaaa aatgcaaaaa acctgttata attgaggatg
     1921 atggtttatt cattgactcg ttatctggtt ttccaggtcc atattcatct tatattttca
     1981 acacaattgg aaataatgga attttaaagc tgataggaga taatagagat gcacaatttg
     2041 tagcaataat tgctttttgt gattctagta acgaaccagt attgtttgaa tccagtgtgg
     2101 caggaaaaat ttcgaaaaat attcaagatg gaggttgggg ttatgatcca atctttattc
     2161 cagaaaaaca aaataaaact tatgcagaat tatatgataa aaataaactg tctcatcggt
     2221 atgaggcttt gaaaaaattt gctaactggt ttaacagtaa gcaggaataa aacgatctac
     2281 aaatcgtttc ttgttttgta atatcactat tctagagatt tcactttcat ccataactgt
     2341 aaaggcgcca ttttcttgag ataatttatt tgcaaaatgt ctaacttcat ccatttcaag
     2401 catatctgaa tattctagtc tgttgattga acgtccaata tgcatgtatg attttatctc
     2461 aataaaatgt gcatttgatt gttggagtaa agatgcaaag acaggaatca tctcttcacc
     2521 atcattataa cctctaatca aagtaattcg aagaaccgtt cttgtatcaa gagtagataa
     2581 catctttaga gttctattcc atctttccca tgaatcatca tatctgggtc tgttgatttt
     2641 caaaaacgac tggtaatctg ccgcattagt tgaaagataa agctgggttg gtagagcgtt
     2701 ttcatcttgt agtttctgta tcatatctgg ttcttgacca tttgtgacca aaaaaattga
     2761 ttttgttgct tcaagggatt tcaaatattt gataagttct ggtaattttg gatacatagt
     2821 tggttcccca gacagtgaaa ttgcataatg gtttggaagt aatgattcat ccaatttctt
     2881 tttgtcctga ttaggatcac cataatgtcc cataatcaat tttcttcttt ccgccatcag
     2941 tttggtcata atttcttccg gttcagcaac attatgtcgt tccatcttca ttgaatcata
     3001 gaactccatt ggtctccaac agtaaatgca tctattctca caatacattc cagcaggtga
     3061 aaattccata caactatgtg tactaattcc ataaaacttg tgtttatagc atgttccttc
     3121 attcctaaat gatttttttg tccaatggca taaccctaca gtggaatgat cagctatacc
     3181 atactttgct ttcttcagct gttttacaat agaagatcta atttgaatta attcaggttc
     3241 cttttctaca acttcgccag aacaactcat atttggaatc tcataccaac ttacttaaat
     3301 ttgcacatgt aggaatgctg aaacgtgtat atgtgatatt ttcatcacct atgcatgctc
     3361 aaaacagtta gtgttgcagg tattgaggaa caaggaattc cattcaaaaa agatagtata
     3421 actcttgaag atgcggtagc agaatacgtg gtagagaatg gtacactcac ctggatagaa
     3481 tgtgttgtag atgatatagt taatgagaca cctggtattc ttgaaaaact tcagattgat
     3541 atggatccaa gtgtactact ctcaggatat attacagcat atgaagatat tggcgataca
     3601 cttggtatta tgttaccatt tattattact ggtgatagca gaacacagac tacaccaatt
     3661 ttgattttta tgaaaaagga tctgatcgtt actatccatg atgactatgg tggaaaaatt
     3721 gcaaaactct ataattattc aaattcgttg atgagaaaac ttcctaaaga atctgagcag
     3781 tgggcggaca ggcaaaccat tttgttattt agattaatgg atgaagttag tgaaacgaac
     3841 ttttctattt taaggacaat tcttgaacaa gcagaacaac tagaaattga tatttcaggt
     3901 gctagacaaa tggatcgtaa tatatcggac gagctctcaa acatgaaaag atcattgtta
     3961 acatttctta gtgcagtatg ggctacacat gatactgtca gaaatgtgaa gtatggtgac
     4021 cctgatatgt taactgatga tgatgatata ttagaaaaat ttgaagttat tttgggacgt
     4081 cttgaccgcc aaattcagat ggctgaaaat gttatggaga taatttctac tggagtaact
     4141 gtcatacaaa ctgaaacttc taatcagtta acaaagttga ttgtttggtt gacagtggca
     4201 gcaactgctg ttttggttcc taacacaata gctacggtat taggaattcc tgatcttcat
     4261 atttcattag cttgggttat tccaatacta attatttcaa cagttatttc tgtaattgtt
     4321 acttatcgtt ggacaaagca atggagagtt aatccattta ggtcaggaaa attccataca
     4381 tttcggaaaa aatttagccg taaataacca attttttaat gccatggaaa gaggtaacta
     4441 acgccagtgt atactaatgc tacagtgatc gccatagcaa caaaccaacc tttaccgttg
     4501 ttcatatcag acacattttg gtagtgaatt ataaacctaa agacaatttt tccgtgtaaa
     4561 aatcatcagt aaaagttaag attattatca caaatcaaca gatgattatt gtggccctcg
     4621 tagtacagtg gttagtatag aggattgtgg atcctcggac aggagttcga ttcccctcga
     4681 gggccttttt ttgttaaaat ataccgaatt tactggattc catctcttct ttgatggcaa
     4741 atttaacatg gagatttttc tgtttagttt tctgtgtaag ccatgtcaaa aatttttttt
     4801 ctaatccttt tcctttcaat ttgtaaaaat catctcccat ttgatcagat agtttttgga
     4861 tcaggtttag gcttactgat acaacataaa aatgccaacc gaaagcaaat tccgagtctg
     4921 tcatacggaa ttcattttct ccatgaacca tctcttctag cattgtgagt atagttgtaa
     4981 tctccttact agattcatca taatttgtag cagatacgtt gatgttcaat cgttcttcca
     5041 taatcagact atactaaatc aaaataaaag gttaatgtta ttttaacaaa tgttcaactt
     5101 caatgttaaa gaacgtcttt aggattccca tgtaattctt aattgggatt ggtatttcat
     5161 ttccacatgc aattccaaga ttttttgcat taatccaaat caccagtgat tgtaagatag
     5221 tcagaaatct attattttct atatcagaca aactaattgc cttggtatat ctttctgcat
     5281 attcccatgc attgacaaaa tcaatacatt ttgcatcaaa aatttctaat aattgttctt
     5341 taagatttgc cgttttgaaa ggaacttgat ttacaatata cggaaactct actttagatg
     5401 gaagacattg aggaagtggt ccccaaagtg taattattct acaaccatct ggaagatcct
     5461 taaatttttt catcatattt tcaattaaat cattatcagt aaaccaaaat agtataactg
     5521 ttgcgtcatc cattttcgca gttaaaatat cctcacaaaa aaactcccac ctatccaatt
     5581 ttattttttt accatagtca ttagcctgtt gaattttttt tgtatcatta tctattccaa
     5641 ctgctttttt cacatggaac tcatttctag ctataaaaat ccctttacca tcaccacatc
     5701 ctaaatgata aaaaatatca ttttcatcta attctaaaaa ctcaaatatg tttctaaaag
     5761 agtgttcagg aagttgaacg tcatctccag aaattatatt atcaggtaaa gattttatgt
     5821 attcttctat cttcatcaat aattgtaagt atttcagaaa tttattctct tatattctcc
     5881 aatgttgata cagcctgcca taaagaggtt ctaatataag acggtagatt tgggtcttgg
     5941 gttatatcat caagtaaact gatcgtatta gcagcacgaa cagatacaga atattcttta
     6001 gattcaagat ctataaccat attagcaata ttttttcgaa tatttcttgg tgttgaagga
     6061 tttgtagcta tctggtttag tgttgatata gtattattca tcaattcctg attctgttct
     6121 ttagaaacca ttcttatttt attactttaa catcttaatt ttaagtctac gtctctatca
     6181 aaatattgtc ttcttcaaca atcacattgt atacttctaa atcattgatt tttgctggga
     6241 attttattaa ttttccattt ttacagtcaa attgtgcacc atgccaatca caaattattg
     6301 ttgaatcctc aatcttacct tctgaaagac tagcaccagc atgagtacat gtatcattta
     6361 ttgcaaaata atctccccca atatttgcaa caactatatc ttttccatta acagatatct
     6421 ttttcatttg accaacgctg atctgagttg ttttacacac aaaaattttt tccatattgt
     6481 aatttggttc aattatttta aatcatttct aatcaacaaa tttgacagtg tttgtaattt
     6541 taccaagttt ttctatttcc atttctattt tatctccatc cttaaggaat ggaatgtctg
     6601 gattattcaa ggcaacgcct tctggtgttc cagttgtaat tatgtcacct ttttctaaag
     6661 tcataacttt gcttaattta gatataatag atgatatact aatgaacata ttctttgtag
     6721 atgaatcttg tctgatttga tcgtttactc ttgtcactaa tcttaaatcc tgtggatttg
     6781 gaatttcaga cgcagtagtt atgtatggac cacatggtgc aaaagtatca aatgattttc
     6841 cacgcgtaaa ctgtttgtct tgcatttgaa tatctctagc agagacatca ttgaaaacca
     6901 tatatccaaa aatacaatct tttgctttat tttcatctac atttttgatt tttttaccaa
     6961 ttataacagc aagctcaatc tcataatcta attgtttgac aaaacttgga cagattatat
     7021 tagatcctgt tccacataat gatgttcttg gtttgaaaaa gattacagga tcctttggag
     7081 acataacatt attctccttt gcatgatttg ggtagttaaa agtgagacaa ataatttttg
     7141 atggatttgg taacggctca acaatctgga agtttttaag attatcctga aatgatgaat
     7201 cagatatttt attttctatt tcattatacc atccatcaaa aaggaaatct ctgatggaaa
     7261 ggggaagagg aactcctgtt tcatacgtta taatttgcct tgttgcaact ttatcatttt
     7321 ttattatacc ataagtttct ttaccatctt ttagtaatcg tgcaattttc attttagatc
     7381 atttatgtgt gaaaatagct tgattcatag aatcctttcg acaatatccg aacctgataa
     7441 actgtatttc agccccatcc ttcaattcta aataatgggg ttctgtataa acatcaactt
     7501 cctctaaact tccctcatta aatttatcat caataaacag ttgatgcggt atgagaattt
     7561 tcaatttgtg ggcatttttt tgacaaatcc attgaatttt cctgaaactt acgtcatgat
     7621 tatcatttgt aaattctgca gttattttcg agtttataga tgtgattttt acattgccca
     7681 atcccattaa gcgtaattga tcattgattt tcagatcttt aacatcttca gacgataaca
     7741 agatgtcacc ttctaccgtc acttctctaa cacccatatc attggatgga tgattaggta
     7801 attttacaac cgaatgcggt aggttactta ctgtcagtat tttaggagat tttaccatga
     7861 ataatctaat actgtttcca tcaacaattt ttctgttaaa tgcttcaaga gaatcaaatg
     7921 gtgctaaagt attggatttc gtaaagccta aggataatgt aaattttcta attgcttcag
     7981 gtgttattcc ttttcgttta agtgcttcaa gtgttggtaa tctggggtca tcataccatg
     8041 aaacttttcc atcatctata agcggcttta agattctttt tgaaactggc attcctttga
     8101 attcaagtct tgaaaattcc cccatttttg gttttctcat gtttaacgca tctagtattt
     8161 cataatacaa ttcatttctc aactcatatt ccttacttct aaaggcatga gtcacatcat
     8221 ccaaactgtc ctcgattgca actgcaaaat cataacttgg ccaaactctg tatttttttt
     8281 ccaatagtgg atgtcttgca tctattattc taaacagtat aggatctcgc atcacagtat
     8341 tcttggattc catatttcca cgaaatctaa caattgcctc accaggtttg tatttatgaa
     8401 acatcttgtc ccatccgtct tcattttgtt taggatcgct ttcactgcat ttacaagata
     8461 tcatttcttt tctgtttttg cttatttcat cacgcttgca cgtacaaaca tatgcgaggt
     8521 tttggtttaa cagatctttg catttatcat aaaataattc aatatcatca gaagtatttt
     8581 ttattttatc atattttatt ccaagccaat ctagtccaac ctttatagct gcccaatatt
     8641 ctagtctctc attttctgga tttgtgtcat caaatcgtag cacatttttt ccaccgtaca
     8701 ttttgctata ttcatcattg ataatagccg ctttggcatg acctatgtgg ggatagccat
     8761 ttggttcagg agggaatcta gtcacaacat taccctgttc agcatcttct agaggaggca
     8821 agcctattct gtcctctttg actttttctt tcaccttcaa aatttcagga aaattattct
     8881 caatctctat tttttgctgt tctattggta tagaattcac ttcagaaaca attgaagcaa
     8941 tttcaggcat aacgtcttta actttatttt taagttcagg gaatgatcct aaaactttgg
     9001 ataagacaat tttgtctttt gttccgccat catgctcaac agcattttgt aacgcaattt
     9061 ttctgatctc tttttttaaa tcattatcca acctataaac tcctttttgt aacaaaacta
     9121 agtaatgcag ccatctcatt tttggctgaa ccagtatatg aggaaataga tttttcagct
     9181 cgctgtgcat attttagtgc tatatctctc acttcttttt ctactcctaa tgaacgaatc
     9241 acatttactg ccaagttaat ttgttgtttt gaagctccag agtttccgaa aactcgcatt
     9301 atttttgttc tattctgacc tcgtgccttt ttaattgcca gaataattgg aagtgatttt
     9361 ttaccttctc gtatatcatt accaactggt ttttttgtaa tcttactatc accaatgata
     9421 ccaattagat cgtctgtaat ttgaaatcca attcctaagt tccttccaaa cgctgacaaa
     9481 tttttcacat ctttttgttt tctttttgca caaattgctc ccatagcaca tgaaacttca
     9541 aagagtactg ctgttttctt ttcaatcatt ttgatgtatt ccttttcagt tggaattttc
     9601 ttattttcag caaattttat gtcgtaaacc tgtccctcac aaatctcgat acatgccttt
     9661 gataattttg atactaactg tgttgtgtaa ttagaagata attttgattt agatgaaata
     9721 gtattgtatg cttttgaata caaaacatcg cctgctagaa tagctaatgg aatatcaaat
     9781 tttttatggg tagttgtaac tccatgtctc atctcatcat tgtccataat gtcatcatga
     9841 accagtgtaa aattatgaat catttcaacc gcacttgctg cagatattgc atcagactgt
     9901 tttccaccaa gcatttggca gctcttcaaa accaaatatg gtcttagacg cttaccacca
     9961 tgaatgatta ggtgagaagc cgcatcatac agctgttttg gatcaccatt gagttccttt
    10021 cttaggaatt tattgacgaa ttttgcattt ttcttgattg aatttgaaac tttcattcta
    10081 ctaatctcca attgttatca ggtatgtagt gtttaattgc attttcatat acagatttca
    10141 aatcatctga agcccatttt gtgattaagg aattgaattt ttccagtgta ttagggtcag
    10201 aatttgcaag aaaataaagt gcaattacca tccatggaca atatacgtcc ctattacgtt
    10261 caagttcatt tttcaattca tcaggattaa tccattttat ctcagaaatt tcatctttga
    10321 tcatagatga ttcatcaaaa ctatctattg taccaatcaa cgtgccgcaa atttcatttt
    10381 cagaaccaac atccttgtat ggaacatgat attcgaattt attcacatag ttcatcttac
    10441 agctaattcc aatctcttcg ggcattctac gctcagcgga tgatgcatat gtttctgatt
    10501 ctctgggatg acttgcaaca gttccgtccc aatcatttgg ccaaagcatt ttaccttcac
    10561 ttcttttggt caagagtaat tttccttcat tattgaatat cagtgcggta aatgctctgt
    10621 gaagctttcc gtttggcaaa tgacatttta ccttctcctc ttttccaata ggattatcgt
    10681 tttcatccac tagaataaca tattcttcca ttttattacc tgaatagtgt accttcgaat
    10741 tttttaccag aaacagcgct agctattctt tgtggtttgt taccgtttac aaaaaatact
    10801 ttcagaccag attttgacat cttcgttgct tccgtaattt ttcgttccat ccctcctgtg
    10861 acatccattt tgtttttact tatttccggc ttctcctttc tgaaatcata gatcaacttt
    10921 tttgatttaa tgttagaata gacaccatct acgtttaaga cgaaaactga taatcttggt
    10981 ttcaaaactt ttgcaatcgt agtcattatc acatctccag ataaaatgta agattttttc
    11041 tgaccatacc ataatgcatc accatatgtt acaggggtaa gcccagattt tgcaatctcg
    11101 ccaatttttt caaccttgct tctaattggt ttacttccat tcataaaatc agttggaggt
    11161 aggcagtacg ggtttaatct gttttttacg gcactatcca aaataatctt gtttagttct
    11221 atcatggaat tttttacgac tgaaactccc ttcaaatcat attttgcagg ttttgtatgc
    11281 ataccatatt tcacggacca gtaatgacca tacgagccac caccgtgaac cagaatcttt
    11341 ggctcattga ttttcttgat ttgttttaga atgttgtcga ttg
//
LOCUS       DASCPJ010000137         3748 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002147171, whole genome shotgun sequence.
ACCESSION   DASCPJ010000137 DASCPJ010000000
VERSION     DASCPJ010000137.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002147171"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="QGF01_06605"
     CDS             <1..330
                     /locus_tag="QGF01_06605"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HJN20587.1"
                     /translation="ITELYLYDVCAFRDTSQWNVFRELFSQNKVDGIIFTSASSVRAF
                     VEIMTKDYEQTELVGALHKTSIIAIGPFTADELKKLNVENVIADVHTVSGAFDAVRKA
                     FSLAEAI"
     gene            382..1782
                     /gene="sufB"
                     /locus_tag="QGF01_06610"
     CDS             382..1782
                     /gene="sufB"
                     /locus_tag="QGF01_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HJN20588.1"
                     /translation="MTTENLDMDYSQYDFKDSTEMYVHLSKKGLTKDTVKEISKMKDE
                     PQWMLDFRLRSYEIFMKKPMPRWGGDLNRIDFQNIYYYAKASEKTEKNWDDVPENVKN
                     TFDKLGIPEAEKKFLAGVGAQYESEVVYHNLRDDLAKQGVLFLDTDSALKEHPEIFKK
                     YFGKIIPPEDNKFAALNSAVWSGGSFIYIPPGVKVDMPLQAYFRINAENIGQFERTLI
                     IADEGSEVHYIEGCTAPVYSSESLHSAVVELVAHKDAKLRYTTIQNWSNDVYNLVTKR
                     AYAYEGATVEWIDGNIGSKLTMKYPGIYLMGRKAYGETLSIAFAGKNQHQDTGAKMVH
                     LAPDTTSKITSKSVSRANGRSTYRGLLKVKKGATNVKSTVRCDALLLDDTSKTDTYPY
                     MEINQEDATVTHEATVGKIGDEQIFYLMSRGFSEEEALSLIVNGFMEPFTKELPMEYA
                     VELNRLIKLEMDNSVG"
     gene            1805..3205
                     /gene="sufD"
                     /locus_tag="QGF01_06615"
     CDS             1805..3205
                     /gene="sufD"
                     /locus_tag="QGF01_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HJN20589.1"
                     /translation="MSQLALSSIDSSLIEEISSKNNEPNWLQEYRKNSLSIYHNLPAE
                     VSPLYNKYTDVRRMNPEQVSLSTNSDSSIPDFLSKRLEEIKNEISIVQIGSNIYSINI
                     DDELKSKGLVISSLDDALQNNSELIQKTLKDSNSEEDKYTALNNAAFNSGIFIHIPQN
                     LVVDKPIHFVSCLSLDGISTISRNMIIAEKNTQASVVQELYAPKTSKQQAYLELLNTR
                     VDDNSKLDFTTLQIMDQSSINFSTRRTHLGQDSKINWYLGLFGSMLSRYRIDYDLNGA
                     GSVVNDAEVVFGNNDQSFDLNTTVNHNAQSTEGKVVEKSILKDTSKSLFKGMIRINEN
                     ASHSNSFLSGRSILLSKGAKSDSIPGLEILTNDVKATHSASVAQLDDEQIFYLGTRCL
                     SYSEAERIIVEGFLEPLSRTMSHQVRAWISNIIESKWTNKELTINMDENLKSIVEVEE
                     TRYNENEEVEQHYKYR"
     gene            3219..>3748
                     /locus_tag="QGF01_06620"
     CDS             3219..>3748
                     /locus_tag="QGF01_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJN20590.1"
                     /translation="MQNSTQSLDTQCRSDFPVLKRRVRDDKALVYLDNAATTQKPIQV
                     IDAITNYYKNHNSNIHRAVHALAEESTEAFEATRSKIAKFLNIVHSEEIIFVRGTTEA
                     INLIAYAWGRDNVQKGDIIVTTEYEHHSNIVPWQVLTQETGAELRYVDIDDNGELMLE
                     QLDEHLATGKVKLVAV"
BASE COUNT         1348 a          579 c          706 g         1115 t
ORIGIN      
        1 ataactgaat tgtatctgta cgatgtttgt gcatttcgtg atacgtcaca gtggaatgta
       61 ttccgcgaac ttttttcaca aaacaaagtt gacgggatta tttttaccag cgcgtcatca
      121 gttagagcat ttgttgaaat catgacaaag gattatgaac agacagaact tgttggtgcg
      181 ttacataaaa catcaatcat tgctatagga ccatttactg cagatgaatt aaaaaaactt
      241 aatgtagaaa atgtaattgc agatgttcat actgtttcag gcgcttttga tgcagttagg
      301 aaagcatttt cattagctga agctatttag ctctccctaa aattccacgt ctttttataa
      361 catattttct aaatctttga tatgactact gaaaatcttg acatggatta ttcccagtat
      421 gacttcaaag attctactga aatgtatgtt catctcagta aaaaggggct cacaaaagat
      481 accgtaaaag aaatcagtaa aatgaaagat gaaccacagt ggatgcttga ttttagactt
      541 agatcatatg agatttttat gaaaaaacca atgccacgat ggggtggtga tctaaatagg
      601 attgattttc agaatatcta ttattatgca aaggcatcag aaaagacaga gaagaattgg
      661 gacgacgttc cagaaaatgt taaaaataca tttgataaat taggaatccc ggaagcagag
      721 aaaaagtttc ttgcaggcgt tggcgcacaa tatgaatcag aagttgtata tcataattta
      781 agagatgatt tggcaaagca aggcgtctta ttcttagaca ccgatagtgc tctcaaagaa
      841 cacccagaaa ttttcaagaa atactttggg aagataatcc ctccagagga taacaagttt
      901 gcagctttga acagtgctgt ttggagtggt ggttcattca tctacattcc tcctggtgtc
      961 aaagttgaca tgccattaca agcatacttt agaattaatg cagaaaatat tggacagttt
     1021 gaacgtactt tgatcattgc tgatgaaggt tctgaggttc attatatcga aggatgcact
     1081 gcaccagttt attcttcaga atcactgcat tctgctgtgg ttgagttagt agcacacaag
     1141 gatgcaaagt tacgttatac aacaatacaa aactggagca atgatgttta caatctagta
     1201 acaaagcgtg catatgcgta tgagggtgcc actgttgaat ggattgatgg aaacatcgga
     1261 agtaaactta ccatgaaata tccaggaatt tatttgatgg gtagaaaagc atatggcgaa
     1321 actctatcaa ttgcatttgc aggaaagaac caacatcaag acactggagc aaagatggtt
     1381 cacttagcac ctgatacaac gtcaaaaata acatcaaaat ctgttagtcg tgcaaatggc
     1441 cgttctacat atagaggatt gttgaaagta aaaaagggtg caaccaatgt aaaatctact
     1501 gttagatgtg atgcattgtt attagatgat acatcaaaga cagatacata tccttacatg
     1561 gagattaatc aagaagatgc aacagttacc catgaagcaa ctgtgggaaa gattggtgac
     1621 gaacaaattt tctatttaat gagtcgtggt ttctcagaag aggaagcatt atctctaatt
     1681 gtaaatgggt tcatggaacc atttacaaag gaacttccaa tggaatatgc agtagaatta
     1741 aacagactca ttaaactaga aatggataat tcagtcggtt agattaaaag attatagatt
     1801 aacaatgtca caacttgctt tatcatcaat agattcatca ctaatagaag aaatttcttc
     1861 gaaaaataat gagccaaact ggttgcaaga atataggaag aattctcttt ctatttatca
     1921 caatttacca gcggaagttt caccattata taacaaatat actgatgtta gaagaatgaa
     1981 tccagaacaa gtatcattat ctacaaattc agattcatca attcctgatt ttttatctaa
     2041 aagattagag gaaattaaaa atgaaattag tattgttcag attggctcta acatttattc
     2101 aataaacatt gatgatgaac taaaatcaaa aggacttgtt atttcttcat tggatgatgc
     2161 attacaaaat aattcagaat taatccaaaa gaccttaaaa gattcaaact ctgaagagga
     2221 taaatataca gcactaaata acgctgcatt taactcagga atttttattc atatcccaca
     2281 aaatctagtt gttgacaaac cgattcattt tgtatcttgt ttatccttag atggaatatc
     2341 tacaatatca cgaaatatga tcattgctga aaaaaatacg caggcttctg ttgtccagga
     2401 actttatgct ccaaaaacat cgaaacagca agcatatctt gaattattaa acacacgtgt
     2461 tgacgacaat agtaaactag attttactac attacaaatt atggatcaaa gctctatcaa
     2521 cttttctacc agaaggacac atcttggtca agattctaaa ataaattggt atcttgggtt
     2581 gttcggttca atgctatcac gatatagaat tgattatgat ctaaatggag caggttctgt
     2641 tgtgaatgat gctgaagtag tttttggaaa caatgatcaa tcatttgatc taaatacaac
     2701 tgttaatcat aatgcacaat caacagaggg gaaagttgtt gaaaaatcaa ttctaaagga
     2761 cacatcaaaa tcattgttca agggcatgat tagaattaat gaaaatgctt cacattcaaa
     2821 ttcatttctg tcaggccgct ctattttatt gagtaaaggt gctaagtcag attcaatacc
     2881 aggtcttgag attcttacga acgatgttaa agctacacat tctgcttctg tagctcaatt
     2941 ggatgatgaa caaattttct atcttggtac acgttgctta agctattctg aggctgagcg
     3001 tataattgtt gaagggttct tagaaccact ttctagaacc atgtctcatc aagttagagc
     3061 atggatctca aatattattg aatcaaaatg gacaaataaa gaattaacaa ttaacatgga
     3121 tgagaatcta aaatctatag ttgaggttga ggaaacacgt tataatgaaa acgaagaagt
     3181 tgagcagcat tataagtatc ggtaggtgtg tcagataatt gcaaaatagc acacaatctc
     3241 ttgacacaca atgtagaagt gattttccag tactgaaaag gagagtaaga gatgataagg
     3301 cattggtata tttggataat gcggcaacca cacaaaaacc aattcaggtt atagatgcaa
     3361 ttactaacta ttacaagaat cataattcca atatacatag agctgtacat gctttggcgg
     3421 aagaatctac agaagcattt gaggctacta ggagtaaaat tgcaaaattt ctgaatattg
     3481 tacatagtga agaaataatt tttgtaagag gaactacaga ggctatcaat ttgattgcat
     3541 atgcttgggg aagagataat gtgcaaaagg gcgacattat tgttacaact gaatatgaac
     3601 atcattcaaa cattgttcca tggcaagtac tcacacagga gacaggtgca gaactcagat
     3661 atgtcgatat tgatgataat ggtgaattaa tgttagaaca acttgatgag catcttgcaa
     3721 caggtaaggt gaaactagtt gccgtaag
//
LOCUS       DASCPJ010000138         8427 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002161743, whole genome shotgun sequence.
ACCESSION   DASCPJ010000138 DASCPJ010000000
VERSION     DASCPJ010000138.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002161743"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="QGF01_06625"
     CDS             complement(<1..164)
                     /locus_tag="QGF01_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HJN20591.1"
                     /translation="MKLVIGITGSTGVIYGIRILEKLKEFNIETHLIMSDWAQKCITM
                     ETEYDLNYVKS"
     gene            complement(190..345)
                     /locus_tag="QGF01_06630"
     CDS             complement(190..345)
                     /locus_tag="QGF01_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20592.1"
                     /translation="MNTEMVLVGMILFSGMIAASVKLWLNKRKQQESEYLDSDTNDDY
                     DTDTTLT"
     gene            418..1509
                     /locus_tag="QGF01_06635"
     CDS             418..1509
                     /locus_tag="QGF01_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase family protein"
                     /protein_id="HJN20593.1"
                     /translation="MIKRSDIQKIINEYTDLRIGVLGSHSALEIMDGAKDENFDTIVF
                     CQKGRETPYQRFSRIANEIKVLKKFSDMSSIKNQKMLRDTNTLIVPHRSLTAYLGYKT
                     IENSFKVPIFGNRALFQAEERNNKKNQYYLLRKAGLKHPKIFKNPKDINRPSIVKVQE
                     KNRKLERAFFTVSSFSDYKKKSEEKIRQGVISKTGLRDAVIEEFAIGTYLNFNYFYTP
                     ISKNVDFIGIERRLQTNLHDFNALPAKQQLELDIDPQNIEVGHTPASIRESLLEKVIS
                     MGDKFVAAVKREYPPGIIGPFSLQSVITKDLEMIVYDVSLRVPGNPIVATTSPYTKYQ
                     YGETFGVGRRIAMEIKIAQEDDVLDKIIT"
     gene            complement(1502..1927)
                     /locus_tag="QGF01_06640"
     CDS             complement(1502..1927)
                     /locus_tag="QGF01_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HJN20594.1"
                     /translation="MKIDDAQEQDYELVRITDLGFVDDYGIEGLLLLKADDGREFHMH
                     AFSGEVARHISNFMGEQQPIPTIYKMLEEICEENGIFLVKVKIYESGEALRANLYFTG
                     KKDMVLRNYRASDAVALAVYYKIPILVRNNLLQETISSK"
     gene            1995..2801
                     /locus_tag="QGF01_06645"
     CDS             1995..2801
                     /locus_tag="QGF01_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20595.1"
                     /translation="MEKKISSKKYNDRLFIALGFISIGILFLIYSGIQDFSVTSDSIK
                     ATDRLAIVFYIIMSLSFGAISYGLYRYHHRKVLEGNDNILGNIASITLNKKAKKIFII
                     TFIAYWCFFSITSGMLIYQPDVTFSYHYDAVIPSIVLIPCCGEPGYVPKIIVYMTEHI
                     GFQIIPINLLLQIIVSYLVGFNMSLALSIMSFTKKGSSLTGIGATTGLFIACPTCVGT
                     FLTLFISSSSAIVFTLTITQLQTVFIAITIPILLITPIIIGKKIQKQNNV"
     gene            complement(2827..3117)
                     /locus_tag="QGF01_06650"
     CDS             complement(2827..3117)
                     /locus_tag="QGF01_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HJN20596.1"
                     /translation="MVKKLVKILIDEMDDGMDEKLSSIGYDAYSVKKLRTQGFKLHAD
                     YSVINYAKENKMILVTRDTESGQACDENSIPCILLDNNEIFKIVLEKLKEFT"
     gene            complement(3128..3508)
                     /locus_tag="QGF01_06655"
     CDS             complement(3128..3508)
                     /locus_tag="QGF01_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HJN20597.1"
                     /translation="MNTSIENELLGVVEDILNLDDLMRFAAIIDLDGTIIEGIMKEGK
                     TSLESQKEQEKFCKEASERRQIRESYNDSLGKIRYVHLERENVTQITIYPKTCTILVT
                     MEPELSIEKKLGIITKIKKIASDL"
     gene            complement(3577..4428)
                     /locus_tag="QGF01_06660"
     CDS             complement(3577..4428)
                     /locus_tag="QGF01_06660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJN20598.1"
                     /translation="MKVNPFHLLLWTFRRNDNDVVSLYNTLSPMMQLITDNNMLNFGY
                     WEDENITPDQAQNKLCDLVGRTAELDTANTLLDIGSGLSSPAIEWSTKYPSTKISCVN
                     TNFQQLKFGKEYVKEKIPNIIHQINSTSTLLPFSDNSIERIIALESAQHFKPFSEFIS
                     ESHRVLKDDGILTFAIPVMKKKSNPKNLGILTFTWSSEHYHENFIINETKEKFDIVEK
                     LEIGSHVFEPLTNYYLKNREKLRNILLTQYPSYVEKILYNSLLKMKNASEEKLIEYFV
                     IKCLKNN"
     gene            complement(4683..6878)
                     /locus_tag="QGF01_06665"
     CDS             complement(4683..6878)
                     /locus_tag="QGF01_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HJN20599.1"
                     /translation="MAKYKSTEQVLKIIKDKNQIRNFGVIAHVDHGKTTMSDSLLAHS
                     GIIAPSAAGKALALDYMKEEQERGITIVQANVTLHYKQGDNEYVINMIDTPGHVDFSG
                     RVIRSLRAIDGAVVVCDAVEGIMTQTETVTRMSLEERVRPVLYINKIDRLIKELRLTP
                     EAMQEKLANVVTNFNNLIDTYAEPEYKEKWKVSIQDGSVTFGSAKDKWGFNVDIMKEK
                     GVSFKDVITAYSDEGDVNVLVEKVPLAEAILGMVVKHHPPPHVAQKYRVPKIWKGDLD
                     SDIGKAILNCDDNGPAVMMVVNMQMDPAAGPVAIGRLFSGTLKDGDTVNLIDTHREAR
                     IQSVNFFMGNQREMVGELSAGNIPALLGLTEARAGQTLATTKGIEVFEGIKYVSEPVV
                     QMAIEPKHPKDLPKMIEILKRLTIEDPNLIIKINEESGETIMAGMGVLHLEIATNQIK
                     EAGLELITSEPLINYRETITQPTEPIMAKSPNRHNKIFMRVEPLEPEIAEMCRTGELG
                     EMKDKKEMTKILRDHGWEPDVAKKVMRFDSRGNIMINGTKGVQFIDESSDSILSGFDD
                     VMKEGPLAREQMRNCKFTFTHFVPHEDTAHRGLSQLGPASRRACLASVLLAQSVILEP
                     ILGIEVRVPQDLVGNVASVLSGKRGKVIDMQQKGVISIVIGEVPASETFDISQVMRGQ
                     TAGKAMWNTFFKAWSPIPKSLVAQLIPDIRKRKGLSPEPPKAEEFIDRE"
     gene            7025..7846
                     /locus_tag="QGF01_06670"
     CDS             7025..7846
                     /locus_tag="QGF01_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HJN20600.1"
                     /translation="MEISVGHTPDSDDAFMFYGMLSGKIRSNDFTIKHVIGDIEELNQ
                     KAVNPELDVTAVSVHACAYLPEYTILRSGGSFGIGYGPIVTSNKDMSIDELKNSLIAI
                     PGKMTSAFLLLQLMIGKFNYLPMNFSDIPAAVKSGKVDAGLVIHETQISYMQEGNIKI
                     LDIGEWWDKTTNGLPVPLGINVMKTSFGEASIKKFDRFLQDSIEYGLEHTTDAIDYAM
                     QYGRNKPRDLIEKFVKMYVNNVTVNMGTEGEDSIKKVFEMAKQKNLTPEFDLKIV"
     gene            8058..8276
                     /locus_tag="QGF01_06675"
     CDS             8058..8276
                     /locus_tag="QGF01_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20601.1"
                     /translation="MKLFLDFDPCKECDSFIEDLTTEESLRADDFFRNEKSAQFMRHL
                     TYNHPEVIQAITAEVKSQERKPEFDLYK"
BASE COUNT         2805 a         1432 c         1211 g         2979 t
ORIGIN      
        1 gattttacat aatttagatc atactcagtt tccatcgtga tacatttttg tgcccaatct
       61 gacataatca aatgagtctc gatattgaat tctttcaatt tttcaagaat cctaattcca
      121 taaattacac ctgtactacc agttattcca attactaatt tcatgatatt caatgaactt
      181 tacaatattt taagtaagag tagtatcagt atcataatca tcatttgtat ctgaatctaa
      241 atactcactt tcctgctgct tacgtttgtt taaccataac tttactgatg cggcaatcat
      301 accagaaaat aaaatcatgc caaccaaaac catttcagta ttcattttaa tattttgaaa
      361 gcatcattga ttaaaaatat actcaacttc atattatggt tacaatatcg ataaaatatg
      421 ataaaaagat cagatattca aaaaattatt aatgaatata ctgatcttcg aataggtgta
      481 cttggaagcc actcagctct tgaaattatg gatggtgcaa aggacgaaaa ttttgataca
      541 atagtatttt gccaaaaagg tagagaaacc ccctaccagc gttttagtag aattgctaat
      601 gaaatcaaag ttttaaaaaa atttagcgat atgtcttcta ttaaaaatca aaaaatgtta
      661 agagatacaa acacgttgat agttccgcat cgttccttaa cagcatatct tggatataag
      721 acaattgaaa attcttttaa agttccaata tttggaaatc gtgcactatt tcaggctgaa
      781 gaaagaaaca ataaaaaaaa tcaatactat ttactccgaa aagctggttt aaaacatcca
      841 aaaattttta aaaacccaaa agacattaac agaccttcaa ttgttaaagt acaagaaaaa
      901 aatcgtaaat tagaaagagc gttttttaca gtttcatcat tttcggatta caagaaaaaa
      961 tctgaagaaa aaattaggca gggggttata tcaaaaactg gtttgcgtga tgctgtaatt
     1021 gaagaatttg caattggtac ttatctcaat tttaattatt tctatacacc aatctcaaaa
     1081 aatgttgatt tcattggaat agaaagaaga cttcaaacaa acttacatga ttttaatgca
     1141 cttcctgcta agcaacaatt agagctagat attgatccac aaaatattga agtgggtcat
     1201 acaccagcaa gtattcgtga atctttactg gaaaaagtga tttctatggg tgataaattt
     1261 gtggcagctg ttaaaaggga atatcctcca ggaataattg ggccattctc acttcaaagt
     1321 gtaattacaa aagatcttga gatgattgtt tatgatgttt cgcttagagt tcctggtaat
     1381 ccaattgttg caactacaag tccatataca aaataccaat atggtgaaac atttggagtt
     1441 ggtcgtagaa tagccatgga aataaaaata gcacaagaag atgatgtgtt agataaaatt
     1501 atcacttaga acttatagtt tcttgtaata ggttgtttct aactaatatt ggtattttat
     1561 aatatacagc aagtgcaact gcatctgacg cacgataatt ccgtaacacc atatcttttt
     1621 tccccgtaaa gtatagattt gctcgaagag cttcaccact ctcataaatt tttactttta
     1681 ccaaaaaaat cccattttct tcacatatct cttcaagcat tttatagata gtaggaatgg
     1741 gttgttgttc acccatgaaa ttagatatat gtcgagcgac ctctcctgaa aaagcgtgca
     1801 tatgaaactc tcttccgtca tcagctttta gtaataacaa tccttctata ccataatcat
     1861 caacaaaacc aagatcggtt attcgtacta actcataatc ttgctcctgt gcgtcatcaa
     1921 ttttcatccg ctatcaacta aatcagaaat tgctttctaa taagcataaa ttatagtaat
     1981 aaatttttta atgtatggaa aaaaagatta gttcaaaaaa atataatgat agactgttta
     2041 ttgcattggg ttttatttca attggaatac tttttttgat atattcaggc atccaggatt
     2101 tttcagttac atctgactcg attaaagcta cagatcgact tgctatagta ttctacatta
     2161 taatgagctt atcatttgga gcaatttcat atgggttata tcgttatcat catcgtaaag
     2221 tgttagaagg taatgataac atcctgggaa atattgcaag cataacttta aacaaaaaag
     2281 caaaaaaaat ctttattatc acattcattg catactggtg ttttttttct attacatctg
     2341 gaatgctaat ttatcagcct gatgtaacat tttcctatca ctatgacgct gtaattccat
     2401 ctattgtttt aatcccttgt tgtggtgaac ctggttacgt gccaaaaatt attgtgtata
     2461 tgacagagca tataggattt caaataatcc caataaatct tttgttgcaa ataattgtat
     2521 catatcttgt tgggtttaat atgtcacttg cattaagcat aatgtcattc acaaaaaaag
     2581 gtagtagttt aactggtata ggtgcaacaa ctggtctatt cattgcatgc cctacatgcg
     2641 ttggtacatt tttaacatta tttatcagca gttccagtgc aattgtattt acacttacca
     2701 tcacccaact acaaactgtc tttattgcta ttacgatacc aatacttttg atcacaccga
     2761 tcattattgg aaaaaaaata caaaaacaaa ataatgttta atatctacta aaaatctagt
     2821 tcgtttctaa gtaaattctt ttaatttttc caaaacaatc ttgaatattt cattattatc
     2881 taataatata catggaatac tattctcgtc acatgcttga ccactttctg tatcacgcgt
     2941 aactaaaatc attttattct cttttgcata attaataaca gaataatcag catgcagttt
     3001 aaatccctgc gtgcgaagtt ttttcacact atacgcatca tagccaatac tagatagttt
     3061 ttcatccatt ccatcatcca tctcatcaat caaaattttc accaattttt tcaccattct
     3121 ataattatta caaatctgat gcaatttttt ttatttttgt tattattcct aattttttct
     3181 ctatacttaa ttctggttcc atagtaacaa gtattgtaca tgtttttgga taaatagtaa
     3241 tctgtgttac attttctctt tctaaatgaa catatcgtat tttccctaat gaatcattat
     3301 aactttctct gatttgcctt ctttcacttg cttctttaca aaatttttct tgttcttttt
     3361 gtgattctaa actagtttta ccttctttca taattccctc aataatagtt ccatcaagat
     3421 caattattgc tgcaaatctc atcaagtcat ctaaatttaa aatgtcttca acaacaccca
     3481 ataattcatt ctcgatagaa gtgttcacaa ttatagcata gatggttata atataactta
     3541 taggaaaaaa gatatgtatt tacatacatt aattttttaa ttatttttta aacattttat
     3601 tacaaaatat tcaatcaatt tttcttcaga agcatttttc atttttaata gtgaattata
     3661 caagattttt tctacatatg atggatattg cgttaatagt atattgcgta atttttctct
     3721 atttttcaga tagtaatttg tgagtggttc aaatacatga gaaccaattt ctaatttttc
     3781 tactatatca aatttttctt ttgtttcatt aattataaaa ttttcatgat aatgttcaga
     3841 tgaccatgta aatgttaaaa taccaagatt ctttggattg gatttttttt tcattactgg
     3901 aattgcaaat gtaagaattc catcatcttt tagaactctg tgtgattcag aaataaactc
     3961 cgaaaatggc ttgaaatgct gggctgactc caatgcaatt attctttcta tagaattatc
     4021 tgaaaatggt aataatgtag aagtggaatt aatttggtgg attatatttg gtattttttc
     4081 ttttacatat tctttcccaa atttcaactg ttgaaaattg gtatttacac atgaaatttt
     4141 tgtggatggg tatttagttg accattctat tgctggtgat gataaaccac ttcctatatc
     4201 taataatgta tttgctgtat ctaattcggc agttcttcca accaaatcac ataatttgtt
     4261 ttgagcttga tcaggtgtga tattttcatc ttcccagtaa ccaaaattaa gcatattatt
     4321 atcagttatc aattgcatca taggtgataa tgtattgtaa agactaacaa catcattatc
     4381 attccttcta aatgtccata ataaaagatg gaaagggttt actttcatta attattatat
     4441 tatgattata tgtatataca atttcatatc ctaattgccg aatattggat atatcaaaca
     4501 ttttgaataa cttagtgaat caaaaaatca ttggctctat tataacaaaa aatacatcat
     4561 aaactatgtc aaacagtgat ttttgggttc atacacgtga atgcacagtt gatgattctg
     4621 aagatggttt tatgaatgag atggctttta ttttgacaac attacatgaa gaaggctgtt
     4681 aattactctc tatcaataaa ttcttcagct tttggtggtt ctggacttaa tccctttctt
     4741 ttcctaatat ctggtattag ttgggcaacc aatgattttg gaattggtga ccatgcttta
     4801 aagaatgtgt tccacatagc tttacctgct gtctgaccac gcatgacctg agaaatatca
     4861 aaagtttcag atgcaggaac ctcgcctata actatgctaa taacaccttt ttgctgcata
     4921 tcaatcacct taccacgttt accagaaaga acacttgcaa catttccaac tagatcttgt
     4981 ggtactctaa cttcaattcc aagtattggc tcaaggatta ctgactgcgc taataagaca
     5041 ctagcaagac atgctcttct agatgctgga cctaattgtg acagacctct atgtgcagta
     5101 tcttcatgtg gtacaaaatg agtaaatgtg aacttgcaat ttctcatttg ttctcttgca
     5161 agtggtcctt ctttcattac atcatcaaaa ccagaaagta tagaatcact ggattcatca
     5221 ataaattgaa caccttttgt accattaatc attatattac cacgagaatc aaacctcatt
     5281 actttcttgg caacatccgg ttcccatcca tgatcacgaa gaattttagt catttctttt
     5341 ttatctttca tttcacctaa ttctccagtt ctacacattt ccgcaatttc tggctctaat
     5401 ggttcaactc tcatgaaaat tttgttatgt ctattaggag attttgccat tataggttcg
     5461 gtaggctgtg ttatagtttc acgataattt atcaacggtt cagatgttat aagttccaag
     5521 ccagcctcct tgatttggtt tgttgcaatc tctaaatgta atacacccat tcctgccatg
     5581 attgtttcac cactttcttc gttgattttg ataatcagat ttggatcttc tattgttaat
     5641 ctttttaata tctcaatcat cttcggaagg tccttaggat gttttggttc tatggccatt
     5701 tgaaccacag gttctgatac atattttatt ccttcaaata cttctattcc tttagttgtt
     5761 gccaaagttt gtccagctct agcttcagtt aatccaagaa gtgcgggaat atttccagca
     5821 gaaagttctc caaccatttc tcgctggtta cccatgaaaa aattgacaga ttgaattctt
     5881 gcttcacgat gagtgtcaat aagattaact gtatctccat cctttaatgt gccagaaaac
     5941 aatcttccta tagccacagg acctgcagca ggatccattt gcatattcac taccatcatg
     6001 acagcaggac cattatcatc acaatttaga atggctttcc ctatatcaga atcaagatca
     6061 cccttccaaa tctttggtac acgatatttt tgtgcaacat gtggtggtgg atgatgttta
     6121 acgaccatac ccaaaattgc ttctgcaaga ggtactttct caacaagaac attaacgtca
     6181 ccctcatcgg aatatgctgt aattacatcc ttgaaggaaa ctcctttctc tttcattata
     6241 tctacattaa atccccattt atccttggca gagccaaagg taacactacc atcttgtatt
     6301 gaaaccttcc atttttcttt gtattctggc tcagcatatg tatcaattaa attattaaaa
     6361 tttgtaacaa catttgcaag tttttcttgc atggcttctg gtgtcaatct taattccttt
     6421 atcagacgat caattttgtt tatgtataaa acaggtcgta ctctttcttc taatgacatt
     6481 cttgtcacag tttcagtttg ggtcataatt ccttcaactg catcacaaac aactacagca
     6541 ccatcaattg ctcgaagact acgaattact cttcctgaaa aatctacatg tccaggtgta
     6601 tcaatcatat taattacata ttcattatcg ccctgcttgt aatgcaaagt aacattagct
     6661 tgaacgatag tgatcccacg ttcttgttct tctttcatat aatctagtgc aagagcttta
     6721 cctgcagcag aaggtgcaat tatcccagaa tgtgccaaaa ggctatcact catggtggtc
     6781 ttaccatgat caacatgagc tataacacca aaattacgga tctgattttt atccttaata
     6841 atttttagta cctgctcggt cgacttgtat ttagccatag gcgatatcct tgatgaacat
     6901 ctattaaacc tatttgctga aaatttttaa taaaattgaa ataaaagggc ataatatttt
     6961 caaacatatt acattattca aatcatatct aatattattt ataatcaaat tttagatcta
     7021 aatcatggaa atttctgttg gtcacacacc agattcagat gacgctttca tgttttatgg
     7081 aatgctaagt ggcaaaataa gatcaaatga tttcactata aaacacgtca tcggtgatat
     7141 tgaagaattg aatcaaaaag cggtaaatcc tgaactggat gttacagcag tatctgttca
     7201 cgcatgtgca tacttaccag aatatacaat tttaagatct ggtggtagtt ttggtattgg
     7261 atatggtcct attgtaacat caaacaaaga tatgtcaatc gatgaattaa agaattcact
     7321 cattgcaatt ccaggaaaaa tgacatcagc atttttactc ttacaactca tgattggtaa
     7381 atttaattat ctacccatga attttagtga tattccagct gcagtaaaaa gcggtaaagt
     7441 tgatgcaggt ttagttatac atgaaactca gatatcatac atgcaggaag gaaatataaa
     7501 aattctagat attggtgaat ggtgggataa aacaacaaat gggttaccag tgccattggg
     7561 aattaatgta atgaaaacaa gtttcggtga agcatctata aaaaaatttg ataggttttt
     7621 acaagattcc attgaatatg gtttagaaca cactacagat gcaatagatt atgcaatgca
     7681 atatggacga aataaaccac gagatttaat tgaaaaattt gtaaaaatgt atgtaaacaa
     7741 tgttacggta aacatgggta cagaaggaga agattctatt aaaaaagttt ttgaaatggc
     7801 taaacaaaaa aaccttacac cagaatttga tctaaaaata gtgtaaaaca gcatggatga
     7861 tttgcatgga cgatttattc gtagttttta tattcataat tttgctttaa taatcattga
     7921 aaatgaaatg caaaaataca ggtagaactg tttatgactt ttcaggcctt ctatcaatta
     7981 tcacttatga cgactaagta acttttaact gatcaattta atggagaaat ttataaagca
     8041 tttctctaac tgaatttatg aaattatttc tagattttga tccatgtaaa gaatgtgatt
     8101 catttattga ggatctaact accgaagaat ctcttcgtgc cgatgatttt tttagaaatg
     8161 aaaaatctgc tcaattcatg cgtcatttaa catataatca tcctgaagta atacaggcaa
     8221 ttactgcaga agttaaatca caggaaagaa aacctgaatt tgatttatat aaataaaaaa
     8281 ttcagataga aaattaataa caggtattca atcattatta catattgtta atagacaaaa
     8341 aaatgatggt ttctggtgtg atcatgattc ttgtcggtgg aacactaact ttctatcttg
     8401 ataatatcac acccattggc tatgcag
//
LOCUS       DASCPJ010000139         2623 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002169437, whole genome shotgun sequence.
ACCESSION   DASCPJ010000139 DASCPJ010000000
VERSION     DASCPJ010000139.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002169437"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(136..453)
                     /locus_tag="QGF01_06680"
     CDS             complement(136..453)
                     /locus_tag="QGF01_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20602.1"
                     /translation="MNKFEEELKKGNFITSECHKCDKIVWPPSEYCDNCFGNVNWRKI
                     SENGEIIEWSKKGSDVFCIAEFEKTIRIMGKLNTKNTDAKIGQHIQLSECSFNQGYRF
                     IFM"
     gene            complement(450..1556)
                     /locus_tag="QGF01_06685"
     CDS             complement(450..1556)
                     /locus_tag="QGF01_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HJN20603.1"
                     /translation="MKKVAVVGYGNTKFSTDDIPIESLLLESTKEVFNTTNNLSQDLI
                     DGVIVSTNDNSKYLGAILSELSGIKPKISHTVEHLCSSGTAATVSAFSYIASGLADII
                     LVAGADKSDNPGQVLDWDRSRGEFTRPIYWASMFTKAHKRKFNTTEEELAVVSAKNHK
                     QALDNPYAYSHKAYTIPEIMNSKNITDDLRMLDCSYSCSGSSSILLASEDIAKKFTDS
                     PVWISGIGQKTNSASFTKNDLTNLDTTKSAADIAYNMAKATANEIDVAEIHDAFSICE
                     LMAVEDLGLAKKTTGAEFIRNLFNTENRQINPRGGLIGAGHPLGATGISQIVEITQQL
                     QNKAEKRQVSNANKGLVHNMSAAATSSTVLILES"
     gene            complement(1553..2524)
                     /locus_tag="QGF01_06690"
     CDS             complement(1553..2524)
                     /locus_tag="QGF01_06690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJN20604.1"
                     /translation="MRLSYSLGSLLTIEQVLECSKKLGKLKPDTMWIPETWGMENFSM
                     LSLASKENPTSKIGSSIINIYSRSPSLVAMGAVTVDTVSNGRLVLGLGTSSQPLVEDF
                     HGNKFEKPVKRMEEYVKIIRLIFAGKIINYSGEFFTLKDFSLLIKPPRNDIPIYLAAI
                     NQKMVELAWNVADGVIFYLRPKNELKSTLTSMQAKKKIDASIQIITCVHSDSEKAINR
                     AKKTLAFYVSVGKIYREFLASNGFENETKNIFEEYKKTGLKNNSELVPESMVKELCIS
                     GTPDECRKQLQGFYETGVDLPIIQFNPIDNVQESFDLVTSTFSEESG"
BASE COUNT          795 a          480 c          388 g          960 t
ORIGIN      
        1 ttttttataa tctttaatca aaagtgacat actagcctgt gtttttgaag gatatagaat
       61 agctggtaat tatattatca acaatgtgtt aatgatcgct atcaatacat tgaaaatgat
      121 actaaatttc agaaattaca taaaaatgaa tctgtatcct tgattaaatg aacattcact
      181 taattgaata tgttgaccaa tctttgcatc agtatttttt gtatttaatt ttcccataat
      241 acgtattgtt ttttcaaatt cagcaataca aaatacatca cttccttttt tggaccattc
      301 tattatttct ccattttcag aaatttttct ccaattcaca ttaccaaaac aattatcaca
      361 atattcactt ggaggccaga caattttgtc acatttatgg cattcactag taataaaatt
      421 cccttttttt aattcctctt caaatttatt catgattcca aaattaaaac tgtactagag
      481 gttgcagctg cagacatatt atgaacaaga cccttatttg cattagaaac ttgtctcttt
      541 tcagccttat tttgtaattg ttgggtgatt tctactattt gtgaaatacc agttgcacct
      601 aatgggtgcc ctgctccaat tagaccacct cttgggttta tttgccgatt ttctgtgtta
      661 aacaaatttc taatgaattc tgcaccagtt gttttctttg ccaaaccaag atcctccaca
      721 gccattaatt cgcatataga aaatgcatca tgaatttctg caacatcaat ttcatttgct
      781 gtagctttag ccatattata tgcaatatcc gctgcgcttt ttgtagtatc caaatttgtt
      841 aaatcattct tagtaaaact tgcagaattg gttttttgac caattccaga aatccatact
      901 ggcgaatctg taaatttttt ggcaatatct tctgatgcaa gtaaaattga tgaactgcct
      961 gagcaagaat aagagcaatc taacatacgt aaatcatctg taatgttttt agagttcatt
     1021 atttcaggaa ttgtataggc tttatgcgag tatgcatatg ggttatctaa cgcctgttta
     1081 tgattttttg ctgaaacaac tgctaattct tcttctgtag tgttaaattt tcttttatgg
     1141 gccttcgtaa acatagaagc ccagtaaatt ggacgtgtaa attcacctct tgatctatcc
     1201 caatccaaaa cttgcccagg attatcactt ttatccgcgc cagcaaccag gataatgtca
     1261 gctaaccctg atgcaatata tgaaaatgca gaaacagtag ctgcagtacc agaactacac
     1321 aaatgttcta cagtatgaga aatttttggt tttattcctg ataattctga caagattgct
     1381 ccaagatatt ttgaattgtc attcgttgaa acaattactc catcaataag gtcttgtgaa
     1441 agattgttag ttgtgttgaa tacttccttt gttgattcaa gtagaagtga ctcaattggt
     1501 atatcatcag ttgagaattt tgtgtttccg tatccaacaa ctgcaacttt tttcatccag
     1561 attcctccga aaatgttgac gtaactaaat caaatgattc ctgcacgttg tctatgggat
     1621 taaattgtat tataggtaaa tctacaccag tttcatagaa cccttgtaac tgttttctac
     1681 attcatcagg tgtaccagaa atacaaagtt cctttaccat agattcaggt actagttcag
     1741 agttattttt tagtccagtt ttcttgtatt cctcaaaaat attctttgtc tcattttcaa
     1801 atccatttga ggctaggaac tcgcgataaa tttttccaac tgaaacataa aacgccagtg
     1861 tctttttggc acggtttatt gctttttcag agtccgaatg aacacatgta atgatctgaa
     1921 tagacgcatc aatctttttc ttagcctgca ttgatgtaag cgtagatttt agctcatttt
     1981 ttggtctaag ataaaaaatt acaccgtctg caacattcca cgcaagttcc accatttttt
     2041 gatttattgc agcaagatag attggaatgt catttctagg aggcttgatt aacaatgaaa
     2101 aatcctttag ggtaaaaaat tcacctgaat aattaattat ttttccagca aaaatcagtc
     2161 gtattatttt tacatattct tccatacgtt taactggttt ttcgaattta tttccatgaa
     2221 aatcttcaac taacggttgg ctgcttgttc ctagtcctag aactagtcta ccattagata
     2281 cagtatcaac cgttacggca cccattgcaa ccaaacttgg gctccttgaa tagatgttga
     2341 ttatggacga accgattttt gaagtagggt tttccttact ggctagacta agcatggaaa
     2401 aattttccat tccccatgtt tctggaatcc acatagtatc tggttttaat ttacccagtt
     2461 tttttgagca ttctaatacc tgctcgattg tcaataatga acctaaacta tatgataatc
     2521 tcatatcaac cattattttt taaagtattt agaatatttt tttgacttca tatcatttga
     2581 gcaaatttca atatctttat gcgaatcaat cgagtgccat aac
//
LOCUS       DASCPJ010000140         2392 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001544722, whole genome shotgun sequence.
ACCESSION   DASCPJ010000140 DASCPJ010000000
VERSION     DASCPJ010000140.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001544722"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            176..634
                     /locus_tag="QGF01_06695"
     CDS             176..634
                     /locus_tag="QGF01_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HJN20605.1"
                     /translation="MVKHLIFFISNYNKNEYSLLLLRVVFGLIMFINHGINKITAGMT
                     KWDRLGSALTDLIGFQFLSSFFGFMASFSESIGALLIVFGLFTRSASVLLFTTMLVAS
                     INHLIDNEFPELAILYCILCLVLILSGPGKYSLDYMCFSKYYVRNNIKMK"
     gene            996..1796
                     /locus_tag="QGF01_06700"
     CDS             996..1796
                     /locus_tag="QGF01_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012787.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HJN20606.1"
                     /translation="MRKIKNNIRRNPRFSFKYSIVFLTTLMFVLINNTLNAQSTVDST
                     RIVRPMPEIVKAQTQQAEAEWLKIWNEGPTKIVWDKMPLQEGDKAPDFELENSSNNLI
                     KLSDLWSEKPMLLIFWRHTGCGCGFARADEIRKQYDSITKMGVQVIIITQAEPERATF
                     YAKEQGLKGTLLSDPNYQVYRAYDLLDGNGPQILGTADIDYSIGEKLQKDRNGTQRAL
                     VDNPWLLPGEFIIDKSGLIKFAYRYDNCDDIPEYWQLEAELKAVVNKI"
     gene            1998..2276
                     /locus_tag="QGF01_06705"
     CDS             1998..2276
                     /locus_tag="QGF01_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20607.1"
                     /translation="MEQVNMAAIAHLKSDYEAWENLFLSHEDNQEQVNNGNILYGKAN
                     DNTAILIMKNVDMSAMMERASNPEFQKMVEPYVEYHEFFTLNEMKPPQ"
BASE COUNT          858 a          323 c          441 g          770 t
ORIGIN      
        1 agtagatgat ttttatcaaa gcctccaatc atatccatta gatatcaact atgtacttcc
       61 aagtgagtca ctttcaatag aagatatttt taattagtat tctatttatc gacaattaaa
      121 gaaagcccca cattcgtggg gtttcttgtt tatgggatga tgggtagatt tatgaatggt
      181 taagcatcta atatttttta taagtaatta taataaaaac gaatatagtc ttttattgtt
      241 gagagttgtt tttggactaa taatgtttat aaatcatggt ataaataaaa tcactgcagg
      301 gatgacaaaa tgggataggt tagggagtgc cctcactgat ttaataggct ttcaattttt
      361 aagttcgttt ttcggattca tggcatcatt ttcagaatct attggtgcac tattaattgt
      421 atttggatta tttacccgtt ctgcttcagt tttacttttc acaactatgc ttgttgcatc
      481 aataaatcat ctaattgata atgagtttcc agagttagcg attttgtatt gtatattatg
      541 tttagttcta atcttaagcg gtccgggaaa atacagttta gactatatgt gcttttcaaa
      601 atattatgta agaaataata tcaaaatgaa atgatgattc ttacgttttt gtataagatt
      661 ttcaactcga gaaccttggg ttttttgttt gtgggaggat gggtagattt ggctatgcgt
      721 aggttttgtt attacttata gccaactaca tagaacagag ctaggcatta gagaaacatg
      781 tcaaaccatt aatcatgatg atttataccc atgtcgcaat aaatgatttt ctggatattc
      841 agaatccatt agacactatt ttattaaatt tgaataagta tgataagcga aaacaaaagc
      901 tccttttatc cggaaatata accatctaaa caggaacttt attccacata taacgagtta
      961 tgagcaataa aaacaaccaa caggattaat aataaatgag aaaaataaaa aacaatatta
     1021 gaagaaaccc aagattcagt tttaaatatt ccattgtatt tttaacaaca ttaatgtttg
     1081 tcttgattaa taatacatta aacgcacaat caacagtgga ttccacaagg attgtacgtc
     1141 caatgcctga aattgttaag gcacaaactc aacaagcaga agcagaatgg ctgaaaatat
     1201 ggaatgaagg accaacaaaa attgtatggg ataaaatgcc attacaagag ggagataaag
     1261 cacctgattt tgaattagaa aactcgtcca ataatctaat aaaactgagc gatttatgga
     1321 gtgaaaaacc aatgctgctt attttttgga gacatacagg ttgcggttgt ggttttgcaa
     1381 gagcagatga aattcgaaaa caatatgatt cgataactaa aatgggagtt caagtaatta
     1441 tcataacaca ggcagaacct gaaagagcta cattttatgc taaagagcaa ggactaaaag
     1501 gaactttatt gagtgaccca aattatcagg tatatagagc ctatgattta ttggatggca
     1561 atggtcctca aatattagga actgcggata ttgattatag tattggggaa aaactacaaa
     1621 aggatagaaa tggtactcag agggctcttg ttgacaatcc ttggcttctt ccaggcgaat
     1681 ttattattga taaatctggt ttgattaaat ttgcatatcg atatgacaat tgcgatgata
     1741 ttcctgaata ctggcaactt gaggctgaat taaaagctgt tgttaataaa atatagaagc
     1801 tcataacaaa atgaataaat cattgggcaa gtagtggata aaatggaatt tagagcaata
     1861 aataaaattt attttaaact gaaaatgaag tgcttctaaa tgcccaaatg ggtggatggg
     1921 tagatttggt tggcaaagaa aaaattaata atctagatta cttttaatct gtacaataaa
     1981 aaatagagga taaaaacatg gaacaggtaa atatggcagc aattgcccat ttaaaaagtg
     2041 attatgaagc atgggaaaat ttgtttcttt ctcatgaaga taatcaggag caggtaaata
     2101 atggtaatat cctatacggg aaggctaatg ataatacggc aatcctaatt atgaaaaatg
     2161 ttgatatgtc tgcgatgatg gaacgagcat ctaacccaga atttcaaaaa atggtggaac
     2221 catatgttga atatcacgaa ttttttacac tcaatgagat gaagccacca caatagtctt
     2281 ctctaactga actattaatc ataaagcccc acattcgtgg gggttttgtt tgtatgagca
     2341 ttcttaactt taattgatgt atatacgaat tgccgtaatt gtagtattga tt
//
LOCUS       DASCPJ010000141         2158 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000408446, whole genome shotgun sequence.
ACCESSION   DASCPJ010000141 DASCPJ010000000
VERSION     DASCPJ010000141.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000408446"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            99..425
                     /locus_tag="QGF01_06710"
     CDS             99..425
                     /locus_tag="QGF01_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HJN20608.1"
                     /translation="MEIVRGKRKEICAGALALKYKEMGGRVKYFGKPYTDIYEETMIK
                     ARIKDKDKILAVGDSLRTDILGANNFKIDSALVLTGIHKKIKEIEKICKKTNIYPKFL
                     FNTLKW"
     gene            457..>2158
                     /gene="scpA"
                     /locus_tag="QGF01_06715"
     CDS             457..>2158
                     /gene="scpA"
                     /locus_tag="QGF01_06715"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HJN20609.1"
                     /translation="MRKKNKKNYINWIQKAKKELKNIKLDNLYSKTPDGIKIKPLYTV
                     EDLKDLEHVFSYPGIDPYLRGPMATMYPNRPWTIRQYAGFSTAEESNAFYKANIKNGQ
                     TGLSVAFDLATHRGYDSDHPKVLGDVGKAGVAIDTVEDMKILFNGIPLEKMSVSMTMN
                     GAVLPVLANFIVAGEEQGVSQNKLSGTIQNDILKEFMVRNTYIYPPEPSMRIVSDIIE
                     YTSKSMPKFNSISISGYHMQEAGSTTAQELAFTIADGIEYVKCALSKGLKVDEFAPRL
                     SFFFAIGIDFFMEIAKLRGARLLWSKLMKQFKPKDPRSLMLRTHCQTSGASLTEQDPY
                     NNVVRTTIEAMASVLGGTQSLHTNSFDEALALPTPFSARIARNTQLILAEETGITRTI
                     DPLGGSYFIEKLTNDLIKEAQKMINEINKMGGMTKAVEKGYPKLKIEEAATLKQARID
                     NADETIVGVNKYPPDTKEKVDILNIDNTKVRREQIKKLSTIRKKRNTKKVLASLEALT
                     NAAKKSPNETNLLKYAIDAARNRASVGEISDALETVFTRHRAIIRSVSGVYSSAFEGK
                     E"
BASE COUNT          869 a          269 c          393 g          627 t
ORIGIN      
        1 tcagttgctc tcataataat tgatccttcg tctgatgtaa tgagggcgtt cccgagaata
       61 ggtatggtag atcttccagg aataatttta gatagttttt ggagattgta agaggaaaaa
      121 gaaaagaaat ttgtgcagga gcacttgctt taaaatataa agaaatgggt ggtagagtta
      181 aatattttgg taaaccttac actgatattt acgaggaaac catgataaaa gcaagaatta
      241 aggataaaga taaaatttta gcagtagggg attctttaag aactgatata ttaggagcga
      301 ataattttaa gatagattct gctctagtat taactggtat acataagaaa ataaaagaaa
      361 ttgaaaaaat ttgtaaaaaa acaaatatat atccaaagtt tttatttaat actttaaaat
      421 ggtagtaata gaatttttaa gttagatcaa taagttatga gaaaaaaaaa taaaaaaaat
      481 tatataaatt ggattcaaaa agcaaaaaaa gagttaaaaa atattaaatt agataatcta
      541 tattctaaaa ctccagatgg tataaaaata aaaccattgt atacggttga agatctaaaa
      601 gatttagaac acgtatttag ttatcctgga attgatcctt atcttagggg tccaatggca
      661 actatgtatc cgaatagacc atggacgata aggcagtatg ctggattctc tacagcagaa
      721 gaatcaaatg cattttataa agctaatatt aaaaatggtc aaactggctt atcagtagct
      781 tttgatctag caactcatag aggatacgat tcagatcatc caaaagtttt aggtgatgtt
      841 ggaaaagcag gtgtcgcaat tgatactgtt gaggatatga aaatactatt taatgggata
      901 cctttagaaa aaatgagtgt atccatgaca atgaatggag cagtgttacc agttttagca
      961 aattttattg ttgcaggtga agaacaaggc gtatctcaaa ataaattatc cggaacaata
     1021 caaaatgata ttttgaaaga gtttatggta agaaatactt atatttatcc accagaacca
     1081 agcatgcgaa ttgtttctga tattattgag tacacttcta aatctatgcc aaaatttaat
     1141 tctatttcaa tttcgggtta tcatatgcaa gaagctggtt caacaacagc ccaagaatta
     1201 gcatttacaa ttgctgatgg aattgaatat gttaaatgtg cattatcaaa gggtttaaag
     1261 gttgacgaat ttgccccacg tttatcattc ttttttgcta ttggaattga tttttttatg
     1321 gaaattgcaa aattaagagg agcaagatta ctttggtcta aattaatgaa acaatttaaa
     1381 ccaaaggatc ctaggagttt aatgttaaga acacattgcc aaacatctgg tgctagttta
     1441 acggagcaag atccttataa taatgtggta agaacaacta tagaagctat ggcatcggtc
     1501 ttaggaggaa cacaatcact gcatacaaat tcatttgatg aagctttagc tctgcctact
     1561 cccttttcag cgagaattgc tagaaacaca caattaattt tggcagaaga aacaggaata
     1621 actagaacaa tagatccatt aggaggaagt tattttatag aaaagttaac caatgattta
     1681 attaaagaag cacaaaaaat gataaatgaa attaataaaa tgggtggaat gactaaggca
     1741 gttgaaaaag gatatccaaa attaaaaatt gaagaagctg caacactaaa acaagctaga
     1801 atagataatg ctgacgagac aatagtcggt gtgaacaaat accctcctga tacaaaagag
     1861 aaggtagata ttttaaatat agataataca aaagtaagaa gagaacaaat taaaaaacta
     1921 tcaacgataa gaaaaaaaag aaacacaaaa aaagttttag cttcacttga agcgctaaca
     1981 aatgctgcaa aaaaatctcc aaatgaaaca aatttgttaa aatatgcaat tgatgctgca
     2041 agaaatcgag catctgtagg agaaatttct gatgcattag aaactgtatt tactagacat
     2101 agagctataa taagaagtgt atcaggagtt tatagttctg catttgaagg aaaagaag
//
LOCUS       DASCPJ010000142         1791 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000001435418, whole genome shotgun sequence.
ACCESSION   DASCPJ010000142 DASCPJ010000000
VERSION     DASCPJ010000142.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000001435418"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="QGF01_06720"
     CDS             <1..477
                     /locus_tag="QGF01_06720"
                     /inference="COORDINATES: protein motif:HMM:NF018201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20610.1"
                     /translation="LGKMVDWCINKKFNITFIEVMPMDMENTTRLEQYYPLTKVRSFL
                     EKRYTLENTNINTGGPAKYVKIKNSNQKIGFINPLTNNFCESCNRVRLTCTGRIYSCL
                     GHNKFVDLKVALLKSKKYLKDLILKSIYLKPKSHEFEIDKESTNKNLERTMNVTGG"
     gene            489..1265
                     /locus_tag="QGF01_06725"
     CDS             489..1265
                     /locus_tag="QGF01_06725"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003406.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HJN20611.1"
                     /translation="MFNHKKIKINFFPARNRKAIVAVKNFKKKYKDHPIKNADVIVAF
                     GGDGTILSALHNSMKFCKPIFGINRGDYGFLTNPYKKIDLIKRLQKAKKTEMHVLKMF
                     ATKKDGKKSEAMAINEVSLLRNSRHAAKIKIQIDNVVRIKELVCDGILLATPAGSTAY
                     NLSARGQIIPMTSKLLALTPISPFRPRSWRGALINSKSKVTFIILDEKKRSVNVSADF
                     KEIKDIKKVEIKEDRSIKLSLLFDPDHNFDKKVLKEQFSF"
     gene            1269..>1791
                     /locus_tag="QGF01_06730"
     CDS             1269..>1791
                     /locus_tag="QGF01_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HJN20612.1"
                     /translation="MKKKILIIFFILSFLYILWPFINIYRFYVAVKKTDIEFFTNNIN
                     WQSLRSGFKDDLEIIINKKIGRKKNIEKQILKTLLGNSLIEIILEEIVTPENIVLLLN
                     NPKKYNEMLKKEIDNPKINKKKENIKKEKFKLKGPSIKRIRKKVNYVFFINLNTFRID
                     FNHDGYPIKVDLKF"
BASE COUNT          791 a          183 c          247 g          570 t
ORIGIN      
        1 ttaggtaaaa tggttgattg gtgtataaat aaaaaattta atattacttt tattgaagtt
       61 atgccaatgg atatggaaaa tactacaaga ttagaacaat attatccttt gactaaagtt
      121 agatcttttt tggaaaaacg atacacacta gaaaatacta atataaatac aggggggcca
      181 gcaaaatatg taaaaataaa aaattcaaat caaaaaattg gttttataaa cccacttact
      241 aataattttt gtgaatcttg taatagagtt cgtttaacat gtactggaag aatttattct
      301 tgcttaggtc ataataaatt tgttgatctt aaagtagcac ttttaaaaag taaaaaatat
      361 ctaaaagatt taattctaaa atcaatttat ttaaaaccta agtcacatga gtttgaaata
      421 gataaagaaa gtacaaataa aaatttagaa cgcaccatga atgttactgg aggataaata
      481 tatcttttat gtttaatcac aaaaaaataa aaataaattt tttcccagca agaaatagaa
      541 aagcaattgt tgctgtaaaa aattttaaaa agaaatataa agatcatcca ataaaaaatg
      601 ctgatgtgat tgttgctttt ggaggtgatg gaacaatatt atcagctctc cacaattcta
      661 tgaaattttg taaaccaatt tttggcatta atagaggaga ctatggtttt ttaactaatc
      721 catataaaaa aatagactta attaaaagat tacaaaaagc aaaaaaaacc gaaatgcatg
      781 tattaaaaat gtttgcaaca aaaaaagatg gtaaaaaaag tgaggccatg gctattaacg
      841 aagtgtctct attgagaaat tccagacatg cagcaaaaat aaaaatacaa attgataatg
      901 ttgtaagaat taaagaactt gtttgtgatg gaattttact agcaacacct gctggcagca
      961 ctgcttataa cctatctgct agagggcaaa tcatacctat gacttccaaa ttactcgctt
     1021 taacaccgat tagccccttt agaccaagaa gttggagagg tgcgcttatt aactcaaaga
     1081 gtaaagttac ttttataatt ttagatgaaa aaaaaagatc ggttaatgtg agtgcggatt
     1141 ttaaagaaat taaagatata aaaaaagttg aaattaaaga agataggtct attaaattat
     1201 ctttattatt cgacccagat cataattttg ataaaaaagt attaaaagaa caatttagtt
     1261 tctaaaaaat gaaaaaaaaa atattaatta ttttttttat tttatctttt ctttatattt
     1321 tatggccttt cataaacata tatagatttt atgtcgcagt taaaaaaaca gatatagagt
     1381 tttttacaaa taatataaat tggcaatcat tacgatcagg ttttaaagat gatttggaga
     1441 ttataattaa taaaaaaatt ggaagaaaaa aaaatataga aaaacaaata ttaaaaacat
     1501 tgctaggaaa tagtttaatt gaaattattt tagaagaaat cgtaactcct gaaaatatag
     1561 tgttattatt aaataatcca aaaaaatata atgaaatgct aaaaaaagag atagataacc
     1621 caaaaataaa taagaaaaaa gaaaatataa aaaaagaaaa atttaaatta aaaggaccaa
     1681 gtattaaaag aataagaaaa aaagttaatt atgttttttt tattaattta aatactttca
     1741 gaatagattt taatcatgat ggttacccaa taaaagttga tttaaaattt a
//
LOCUS       DASCPJ010000143         1558 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000000235152, whole genome shotgun sequence.
ACCESSION   DASCPJ010000143 DASCPJ010000000
VERSION     DASCPJ010000143.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000235152"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1558
                     /locus_tag="QGF01_06735"
     CDS             <1..>1558
                     /locus_tag="QGF01_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025009.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HJN20613.1"
                     /translation="NQTIGEVNGEKIDYETFNLLVQQTQDFYGLDLINAVNYAWEVGI
                     KNIVLTQELDKLGINAGKDQLEQIVSAEPSIVSNPEFQNDIGLFEFTIFSRYINQLKS
                     LNPTAYNSWKIEEQNVIASAKEKIYFNLITSSITYTDVESNLEYHLENDKVTIDYIKI
                     PFNEIPDSIFKITNSQISNFIKKNKNNYIIDPSRIIEYVFVPDIASPLDENNIRTNLE
                     SIRDGIIQYNDVSKLVDSIEGFKDAKNIVEFVETYSEKPYDSIYKTRDQLSNNFGDIL
                     YGLSKGKTFGPYKDGNYYVLTKMIDKKREGGVNKVLLAYIHQQIVPSNESSNQNYRLA
                     SQIEFDAKTNLSLENDNFFSVRNYETLNPYDSGIPDISDSRSVIKWIYDESTKVDEIK
                     RFTLTDGYLIGKIKEINTERLPGVDDVSDNVKNEILKKIKFDYIKKKYKTPTIESIVE
                     DYNIDIQRASAVTQYDPVLVGAGAEPYIIGASFALEPGEVSNLLLGKEGIYLIHSISK
                     EIAEDLNLNSA"
BASE COUNT          631 a          179 c          248 g          500 t
ORIGIN      
        1 aatcaaacaa taggagaggt aaatggagag aagatagatt atgaaacctt taatttgttg
       61 gtgcaacaaa ctcaagattt ttatggactt gacttaatta acgctgtaaa ttatgcttgg
      121 gaagtaggaa taaaaaatat tgtattaact caagaattgg ataaattagg aataaatgct
      181 ggtaaagatc aacttgaaca aatagtgtca gctgaacctt caattgtttc caatccagag
      241 tttcagaatg atataggtct ttttgagttt actatattta gtagatacat aaatcagcta
      301 aaatcattga atccaactgc atataattcc tggaaaattg aggaacaaaa tgttattgca
      361 tcagcaaaag aaaaaattta ttttaatcta attacatcaa gtataacata tactgacgta
      421 gaatcaaacc tagagtatca tttagaaaat gataaagtaa caatagatta tataaaaatt
      481 ccttttaatg aaattcctga ttcaatattc aaaattacta actcacagat atcaaatttt
      541 attaaaaaaa ataaaaacaa ctatattatt gatccatcaa gaattataga gtatgtattt
      601 gttcctgata ttgcttctcc tcttgatgag aataatatta gaactaatct tgaatcaata
      661 agagatggaa taattcaata taatgatgta tctaaattag ttgatagtat tgaagggttt
      721 aaagatgcaa aaaatatagt tgagtttgtt gaaacttatt cagaaaaacc atatgactca
      781 atttacaaga ctagagatca actttctaat aattttggag atatattata tggattaagt
      841 aaagggaaaa cttttggacc atataaggat ggaaattatt atgtattaac aaaaatgatt
      901 gataagaaaa gagaaggagg tgttaataaa gttttattag cttatattca tcaacaaata
      961 gttccatcta atgaatcttc aaatcaaaat tatagacttg cctcacagat tgaatttgat
     1021 gcaaaaacta atttatcatt agaaaacgat aattttttta gtgttaggaa ttatgaaact
     1081 ttaaaccctt atgactctgg aattcctgat ataagtgatt ctagatcagt aattaaatgg
     1141 atttacgatg agtcaacgaa agtagatgaa ataaaaagat ttactttaac tgatggatat
     1201 ttgattggta aaattaaaga gataaacaca gaaagacttc caggggtaga tgatgtttca
     1261 gataacgtaa aaaacgaaat tttaaagaaa ataaaatttg attatataaa gaaaaagtat
     1321 aaaaccccaa ctattgaatc aatagtagaa gattataata ttgacattca aagagcttca
     1381 gccgttactc aatatgaccc tgtacttgtt ggagcagggg ctgagcctta tattattgga
     1441 gcatcttttg ccctagaacc tggagaagtt tcaaatttat tgttagggaa agaaggaatt
     1501 tatcttattc actctatatc aaaagaaata gcagaggatt taaatttgaa tagtgcta
//
LOCUS       DASCPJ010000144         3238 bp    DNA     linear   ENV 14-DEC-2023
DEFINITION  MAG TPA_asm: Candidatus Nitrosopelagicus sp. isolate Fu_MAG_43
            c_000002042307, whole genome shotgun sequence.
ACCESSION   DASCPJ010000144 DASCPJ010000000
VERSION     DASCPJ010000144.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34270999
            Sequence Read Archive: SRR4465024, SRR4465025, SRR4465026,
            SRR4465027, SRR4465028, SRR4465029, SRR4465030, SRR4465031,
            SRR4465032, SRR4465033, SRR4465034, SRR4465035, SRR4465036,
            SRR4465037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopelagicus sp. (marine metagenome)
  ORGANISM  Candidatus Nitrosopelagicus sp.
            Archaea; Nitrososphaerota.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 7x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/09/2023 01:29:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,355
            CDSs (total)                      :: 1,306
            Genes (coding)                    :: 1,299
            CDSs (with protein)               :: 1,299
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Candidatus Nitrosopelagicus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000002042307"
                     /isolate="Fu_MAG_43"
                     /isolation_source="seawater"
                     /db_xref="taxon:2026808"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="17.04 N 106.54 W"
                     /collection_date="2012-04-08"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            175..891
                     /locus_tag="QGF01_06740"
     CDS             175..891
                     /locus_tag="QGF01_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HJN20614.1"
                     /translation="MGGIAGSKKLKRQMAPSFWGIERKNKRFVITVRPGPHKKDRSIP
                     TAVLLRDTIKKVSTLKEAKATIYNGQVNVDGVTRKSLHHSIGLMDVVEFEGSSDIYRL
                     VPQNKRVLTPIIIDTNEKSKKIVKVTSKTTIKSGKIQLGFHDGRTLITDTKVNVDDSC
                     LLQIPEQKILEVLKLENECMVIVTRGINVGQIGRVDKIKEGTFSLPKIINLIINDKKI
                     EIPAELVMVIGKEKPVIKVR"
     gene            896..1417
                     /locus_tag="QGF01_06745"
     CDS             896..1417
                     /locus_tag="QGF01_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HJN20615.1"
                     /translation="MSQQTINTMQKISLNKVVLNMGVGKSGDPIEIARAALQEISGEK
                     PNPRNAKKSQRDWGVRKGEPIGVAVTVRKENAITLLKKLLEAKDNQIKERSFDNEGNF
                     SFGITEHIDIPGVKYNPKIGILGLSVSVSLTRPGYSIRLRSKHKSNIGKNHKISKQNA
                     IDFMKENFGVTIL"
     gene            1417..1596
                     /locus_tag="QGF01_06750"
     CDS             1417..1596
                     /locus_tag="QGF01_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HJN20616.1"
                     /translation="MVKDRSYESTGRTQHRFGRGSRWCKRCGDYTAVIQKYDLSICRR
                     CFREVATSLGFRKNN"
     gene            1606..1998
                     /locus_tag="QGF01_06755"
     CDS             1606..1998
                     /locus_tag="QGF01_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HJN20617.1"
                     /translation="MPAINILANLFTTLYNTEDRRKEQCVILPTSKIALQVLKTLKNE
                     GYIGEFEEIDDKRGGKFKIQLLAKITKCGAVTPRFKVKKDEYNQWEQKFLPSYDRGML
                     IITTNQGVMSHHEAANKGIGGFLIGYVY"
     gene            1988..2545
                     /locus_tag="QGF01_06760"
     CDS             1988..2545
                     /locus_tag="QGF01_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HJN20618.1"
                     /translation="MSTKQTEKMEVGVVIPENVKVRLTGHMLHVEGPLGKTHKNFKKI
                     PVDIQINNDKIILKALGGRKKYYAILNTSKSLIQTLCDGVVNGYTIKMKIVYSHFPIT
                     VKVEGKKVLIENFQGERAHRISKIWNNTKVTPKGDDVIITGHVLTDVTQTAAEIELNS
                     RVKNKDHRVFLDGIYKYSKVVGIEK"
     gene            2575..2865
                     /locus_tag="QGF01_06765"
     CDS             2575..2865
                     /locus_tag="QGF01_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20619.1"
                     /translation="MVLAEEFKKQTIELVEKTLKLYKNAGASPRIGDVWKCKNTGDFL
                     CGFFVGEMIGSALSAFQIFHKREPTSDEHIEIVEVVEKFNDEIMEFFSKFNS"
     gene            complement(2870..3001)
                     /locus_tag="QGF01_06770"
     CDS             complement(2870..3001)
                     /locus_tag="QGF01_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJN20620.1"
                     /translation="MSSSYKNSSTNISKEKEKQENLGKAMSIIGISILIVITLLKII"
     gene            3148..3221
                     /locus_tag="QGF01_06775"
     tRNA            3148..3221
                     /locus_tag="QGF01_06775"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3182..3184,aa:Asn,seq:gtt)
BASE COUNT         1301 a          404 c          588 g          945 t
ORIGIN      
        1 agctaagact aaacctacag aagctaagac taaacctaca gaagctaaga ctaaacctac
       61 agaagctaag actaaaccta cagaagctaa gactaaacct acagaagcta agactaaacc
      121 tacagaagct aagactaaaa caaaatctag taataaaaag gaggatgaaa aataatgggt
      181 ggtattgctg gtagtaaaaa attaaaaaga caaatggcac catcattttg gggtatagaa
      241 agaaaaaaca aaagatttgt aattactgtt aggccaggac ctcataaaaa ggatagatct
      301 attccaactg ccgttttatt acgtgataca ataaaaaaag tttcaacctt aaaggaagca
      361 aaagctacta tctataatgg tcaagtaaat gtagatggtg ttacacgaaa atctttacac
      421 cactctattg gactaatgga tgttgtagaa tttgaaggta gttcagatat ctatagatta
      481 gtaccacaga acaaacgagt attaactcca attataattg acactaatga aaaatctaaa
      541 aaaatagtaa aagtgacaag taaaacaact attaaatcag gaaaaataca attaggtttc
      601 catgatggta gaacgttgat cactgacact aaagtgaatg ttgatgattc gtgtttatta
      661 caaatacctg aacagaaaat tttggaagta ttaaaattag aaaatgaatg tatggtaata
      721 gtgacaagag ggattaatgt aggacaaatt ggacgtgttg ataaaattaa ggagggtaca
      781 tttagtttac caaaaataat taaccttatt ataaatgata aaaaaattga aattcctgct
      841 gaactagtaa tggttattgg aaaagaaaaa ccggtgataa aagttaggta ataatatgtc
      901 tcaacaaaca ataaatacaa tgcaaaaaat ttcgttaaac aaagttgtac taaatatggg
      961 tgtgggaaaa tcaggcgatc ctattgaaat agctagggct gcattacaag agatcagtgg
     1021 agaaaaacca aatccacgaa atgcaaaaaa atctcaaaga gattggggag taagaaaagg
     1081 agaacccatt ggtgttgctg tgactgttag aaaagaaaat gcaataacat tattaaaaaa
     1141 attactagag gctaaagata atcaaattaa ggaaagatct tttgataatg aaggaaactt
     1201 ttcatttgga attactgaac atattgacat accaggtgtg aaatataacc ctaagattgg
     1261 tattttaggt ctaagtgtat ctgtatcact tactagacct ggatacagta taagattaag
     1321 aagtaaacat aaaagcaaca ttggtaaaaa tcataaaatt tcaaaacaaa atgcaattga
     1381 ctttatgaaa gaaaattttg gggtgacaat cctataatgg taaaagatag atcctatgaa
     1441 tcaactggaa gaacacagca taggtttgga agaggctcac gttggtgtaa acgatgtgga
     1501 gattatacag ctgttattca aaaatatgat ttatctatat gtagacgttg ttttagagaa
     1561 gttgctactt cattgggatt taggaaaaat aattaggtga ataacatgcc agcgattaat
     1621 attttagcaa atttatttac aacattatac aatactgaag atcgaagaaa agaacaatgt
     1681 gtgattttac caacatcaaa aattgcgtta caggtgctta aaacattaaa gaatgaaggg
     1741 tatatcggtg aatttgaaga aattgatgat aaaagaggtg gaaaattcaa aatacagctt
     1801 ttagcaaaaa ttacaaaatg tggtgcagta actccaagat tcaaagttaa aaaagatgaa
     1861 tataatcaat gggagcaaaa atttctccct tcatatgata gaggtatgtt aattattaca
     1921 acaaatcagg gtgttatgtc acatcatgag gctgctaaca aaggtattgg aggatttttg
     1981 attggatatg tctactaaac aaacagaaaa aatggaagtt ggggttgtaa tccctgaaaa
     2041 tgtaaaagtt agattgactg gccatatgct acatgtggaa gggccattag gtaaaacaca
     2101 taaaaatttt aaaaaaattc ctgttgatat acaaataaac aatgataaaa taatcttgaa
     2161 ggcacttggt ggaagaaaaa aatattatgc tatacttaat acatctaaat cattaatcca
     2221 aactctttgt gatggtgttg taaatggtta tacgataaaa atgaaaattg tttattctca
     2281 ttttccaatt actgtaaaag tggaaggaaa aaaagtactg atagaaaatt ttcaaggtga
     2341 aagagcacat agaatttcta aaatatggaa taacacaaaa gtaactccaa aaggtgatga
     2401 tgttataata actggtcatg tacttactga tgtaacacaa actgctgcag aaattgaatt
     2461 aaattcaaga gtaaaaaata aagatcatcg tgtttttctt gatggaatat acaagtattc
     2521 taaagtagtt ggcattgaaa aataaaaatc tggattaata aattacgata aaatatggtg
     2581 cttgctgaag aatttaaaaa acaaacaatt gaactcgtag aaaaaacatt aaagctttac
     2641 aaaaatgctg gagcatctcc tagaataggt gatgtgtgga aatgcaaaaa cactggtgat
     2701 tttttatgtg gattttttgt tggggaaatg attggttctg cattaagtgc atttcagata
     2761 tttcataaaa gagaacccac atcggatgaa catatagaaa ttgttgaagt ggttgaaaaa
     2821 tttaatgatg aaataatgga atttttttcc aagtttaata gttaaatctt taaattattt
     2881 ttaatagagt gataacaatt aagatagaaa tgccaattat agacatagct tttccaagat
     2941 tttcctgttt ttccttttct ttggatatgt ttgttgagga atttttataa ctacttgaca
     3001 tttttgataa aatctctaat atcaggtatt atctaaacca aactgtcagt atcgtaaata
     3061 aactgaatat ggtctaattt tttacaattt tccatttttt acacgagtcg taaagattaa
     3121 atcgcttctt ctaaaggtaa aaaccatgcc gccctagctc agcgtggtag agcgtcggac
     3181 tgttaatccg ttggtcgtca gttcaagtct gacgggcggc gtcttttata tttctaaa
//
